LOCUS       DBNGSH010000001         1540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1698166, whole genome shotgun sequence.
ACCESSION   DBNGSH010000001 DBNGSH010000000
VERSION     DBNGSH010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1698166"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="AC16CY_00005"
     CDS             <1..1250
                     /locus_tag="AC16CY_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352183.1"
                     /translation="SARAQLQWADWEGDFDGLEWLNADSEWRDERSLDLARALLTYPF
                     RRAATLSRLLDRPEEVLTRWDSLTRRRRVVVVAASDAHARIGSGSDAAAGIASLLVPS
                     YEQIFRTLSISTPLVRLTGSAEEDARAVTDAIRRGQVYSTIDGLAGPAVFSFSAASGS
                     HRVTAGGALAIEGPVEFRVEANATRGAQIVLLRDGTIVASSAPPLLHETSSPGVYRAE
                     VRLADAPGHPPVPWVVSNPIYVGGNAVAQPPRRERGETERVAIYTDGPASDWRIERSP
                     GSQGSLDVVPRAGGTELSLHYALGEPTESPYVAIVAAAGKGMAGFDRVSFKARAAAPM
                     RVSVQLRVPDGTRDRRWRRSVYLDDMEQTIIVFLDEMTPVGDGSGPLELPAVRDLLFV
                     VDTVNSRPGSSGQVWLDEITFAR"
     gene            complement(1234..>1540)
                     /locus_tag="AC16CY_00010"
     CDS             complement(1234..>1540)
                     /locus_tag="AC16CY_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR3352184.1"
                     /translation="QLAQSGFDVVKQQVALQRKISFPFVTIVMTLIAVPFAVTIGRAG
                     AMAGIGVGIALALVYWTAIQLFSAMGAGGLLSPVLAAWAPNLLFGAAAVYLVLTVRT"
BASE COUNT          246 a          508 c          558 g          228 t
ORIGIN      
        1 gctccgcacg cgcccaactg cagtgggctg attgggaggg cgacttcgac ggcctcgagt
       61 ggctcaacgc agacagcgag tggcgagacg aacgttcact cgacctggcg cgtgccctcc
      121 tgacgtaccc cttccggcgc gccgcgacgc tgtcgaggct gctcgacagg ccggaggaag
      181 tcctgactcg ctgggactcg ctgacgcgcc gccgccgcgt cgtggtggtg gcggccagcg
      241 acgctcatgc gcgtatcggg tccggctcgg acgccgcagc gggcatcgcg tcgctcctgg
      301 tgccgtccta cgagcagatc ttccgcacgc tgtcgatctc gacgccgctc gttcgtctga
      361 ccgggtccgc cgaggaggac gcgcgggccg tgaccgacgc gatcaggcgc gggcaggttt
      421 actcgacgat cgacgggctg gctggcccgg ccgtgttcag cttttccgcg gcgagcggct
      481 cgcacagggt cacggcagga ggcgcgctgg cgatcgaggg gcctgtcgag ttccgcgtcg
      541 aggcgaacgc gacgaggggg gcgcagatcg tgttgctcag ggacggcacc atcgtggcgt
      601 cgtcggcacc gccgctgctg cacgagacct cgtcgcccgg cgtctaccgc gccgaggtcc
      661 ggctcgcaga tgcgccgggg cacccgccgg tgccctgggt ggtatccaac ccgatctacg
      721 tcggcgggaa cgccgttgcg caaccgcccc ggcgcgaacg cggcgagacc gaacgcgtgg
      781 cgatctacac cgacgggcca gcctccgact ggcgaatcga gaggagccct gggtcccagg
      841 gatcgctcga tgtcgtccca cgggccggcg gaacggagct gtcgctgcat tatgcgctgg
      901 gggagccaac cgaatcccca tacgtggcca tcgtggcagc ggcggggaag gggatggccg
      961 gcttcgatcg ggtgtcgttc aaggcgcgcg cggctgcccc gatgcgggtg tcggtgcagc
     1021 tgcgggtgcc cgacgggact cgagaccgtc gctggcgaag gtcagtctac ctggacgata
     1081 tggagcagac gatcatcgtc ttcctcgacg agatgacgcc tgtcggggac gggtcaggac
     1141 cgctggaact gccggccgtg cgtgacctcc tcttcgtggt cgataccgtg aacagccggc
     1201 ccgggtcgag cggtcaggtc tggctggacg agattacgtt cgcacggtga gcacgagata
     1261 gaccgcggcc gcaccaaaca gcaggttcgg agcccacgcg gcgaggaccg gtgagagaag
     1321 cccgccggca cccatcgccg aaaacagctg aatcgccgtc cagtacacga gcgcaagcgc
     1381 gatgccgacg ccgatgccgg ccatcgcacc ggcgcggcca atcgtgaccg caaaggggac
     1441 cgcgatgagc gtcatcacga tggtgacgaa ggggaacgag atctttcgct gcagcgccac
     1501 ctgctgcttg acgacgtcga agccgctctg cgcgagctgc
//
LOCUS       DBNGSH010000002         6584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4245243, whole genome shotgun sequence.
ACCESSION   DBNGSH010000002 DBNGSH010000000
VERSION     DBNGSH010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4245243"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>6584
                     /locus_tag="AC16CY_00015"
     CDS             <1..>6584
                     /locus_tag="AC16CY_00015"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIR3352185.1"
                     /translation="VKNLVDRLEILRTTYRLQSGIRVPWQVISDVEELSWQRLDWSGR
                     SPAQVDTSLDELLRDERTRAVSLETAPTLRLTLATVAATEHYLIVSLPSLCADARSLT
                     NLFEEVVRAYSGAEAAGDEPLQYADFADWHWQLLEEDEQSPARTYWREQRLSASLLST
                     LYRARNGEQPAPFDVAMTASPIDHLTVLSATALAGDQQVPASDFYLACWQALVWRLAG
                     QPEVVTGAIADDRGHDTLRDGCGLMSMTLPVRSQFGEHTRFDQVLEAVARSRRAASDR
                     QFDFSWERDVEGAGRDGEPAFFPLSFEFHALPPARVVGPVTFSVARSFACTDRFAVKV
                     NCVQRGDAVGIEWHFDPNVLSSRQIEHLARHYQALVRSAIDNPRALVLDLDLFDETLR
                     QEIVVGFNGVRSDEPIDRSVHECFEEVASQSPDRTALVYEDQLLSYGELNSRSNRLGR
                     YLRSLGVGSDVPVALCVERSLDMIVGMLGVFKAGGAYVPLDPSLPAERLALLLAESRA
                     PVVVCQPHLADLLSGCAAKLVVLDAGTQRAISQEKAENCLSGVSPANLAYAIFTSGST
                     GTPKGVLVEHRQLVNYVHGVRERLRQPGDVSFATVSSYAADLGNTAIFPALLGGGCLH
                     VVPQDRVLDPGAMADYGERHAIDCLKIVPSHLSALLSAPRPARLLPRRCLVVGGEAST
                     WDLVSRLESLSDDCQIVNHYGPTEATVGVLAYRHERGHIRRDESDTLPLGRPLSNSRV
                     YLLDSRARPVPVGVNGELHIAGAGLARGYLDRPDQTAERFIPDPIAAEPGARMYRTGD
                     LARHLADGNVEFVGRVDHQVKIRGFRVELGEIESALERHEAIDDVVVTVSESPSGDKR
                     LVGYAVRRRGAQVTTDELRDWVRAQLPDYMVPGAIVWLDALPLNRNGKVDRKALPAVE
                     HVTERGPGSPVSPRTLTEETLLGIWCAVLGKTGLGIHDDFFECGGHSLLAMQLITRVR
                     EAFRVDLPLPALFDTPTVAGIAESIETLIGAGTSGVASALKPVSRDRTLPLSFAQQRL
                     WFLEQLEPNSSAYNRPFAFRLTGPLNLESLGQSLEEIVRRHEVLRTTYPTVDGEARQV
                     IAPYAGLRVRLIDLRGVPLQERDLVAHRLAAEDALRLFDLARGPLVRACVMQLNDLDH
                     VLLLSQHHINTDAWSNDVLVHEVSKAFGAFSNGTPSPLAELPIQYADYAAWQREWLES
                     EPARQQLVFWTEEFKAVPPALELPTDRPRPAAQTFHGAARRFRLSRRLAASLRELSGK
                     EGTTLFMTLLAAFQTLLHRYTGQPDILVGSPIAGRTRAEVEGLIGFFVNTLVLRADMR
                     GNPRFSDVLTQVRHRAVAAYENQDLPFERLVDALDLERDLSRTPLFQVMFVLQTDALK
                     SLHLADLVITQADAPIEAAKFDLTLYLSETEQGLNGSLEYNTDLFNASTIDRMWSHFE
                     TLLEGIVADPRRPVLELPLLGDAERRQLLVEWNDTRADYPQGECLHEVIEEQVRRSPD
                     AVAVVFEDRQLTYGELNRKANQLARHLRSLGVGPEARVGLCLDRSLEMVVGVLGVLKA
                     GAAYVPMDPAYPKGRLAFILQDSRATVLLADERSAAVLPDHTARVVSLNPGLQICARE
                     NGENLGPNGDSRNLSHVIYTSGSTGQPKGVAIEQRSVTTLLRWSQDNYSSEELAGVLA
                     STSICFDLSVWELFVPLSVGGAVVLAPTALELPGLLAAQRVTLVNTVPSAIAELLRTG
                     GIPSSVRTVNLAGEPLTSELSDALYSTRTIEKVCDLYGPSEDTTYSTFARRSLGGRAT
                     IGRPLAETRVYLGEAGRAPVPIGIAGELHLGGAGLARGYLDRPDLTAERFLPDALGAE
                     PGRRIYRTGDMARWRDDGSLDFLGRIDHQVKIRGFRIELGEIESALRGHSAVRDGIVL
                     AREDQAGVKRLVAYLTAEGPPPTVTELREHLRDRLPGYMIPAAFVVLDALPLLPNGKI
                     DRRALPAPEQTRPELEEAFVPARTPIEQRLAGIWCRVLGLERVGVHDNFFELGGDSIL
                     SIQIVSRANQDGLRLSAKQVFEHQTIAELAAVAGTAPAVQAEQGRVTGGAPVTAIQQW
                     FFDQQFAEPHHFNQAVLLDINEALDPDILRTAVDALLEQHDALRLRFVERDGGWRQED
                     TAASSTTVSRIDLSHLNDVEQRTALEAETARIQASLTLHAGPQIRVALFDLGARGRRL
                     LLVI"
BASE COUNT         1059 a         2170 c         2205 g         1150 t
ORIGIN      
        1 gtcaagaacc tcgtggaccg tctcgagatc ctgcggacga cgtatcgcct tcagtcgggc
       61 atacgggttc cgtggcaggt gatttcggat gtcgaggaat tgtcctggca gcggctcgac
      121 tggagcggca ggagtccggc gcaggtggac acctcgctcg atgaactgct acgcgacgag
      181 cgaacccgtg cggtgagcct cgagacggcg ccgacgctgc ggctgacgct cgccacggtc
      241 gccgcgaccg agcactacct gatcgtgagc ctgccgtcgc tctgcgcgga cgcgcggtcg
      301 ctgacgaatc tcttcgagga agtggtgagg gcctactcgg gggccgaggc cgccggggac
      361 gagccgcttc agtacgccga cttcgcggat tggcattggc agcttctcga agaagacgag
      421 caatcgccgg cccgcacgta ctggcgcgaa cagcgtctga gcgcctccct cctctcgacg
      481 ctttaccggg cgcgaaacgg tgagcagccg gcaccgttcg acgtcgcgat gaccgcctcg
      541 ccgatcgatc acctgacggt gttgtccgcg acggcgctgg ccggggacca gcaagtcccc
      601 gcatcggact tctacctggc ctgctggcag gcgctcgtgt ggcggctggc cgggcagccc
      661 gaagtggtga ccggcgccat cgcggacgat cgaggccacg acacgctgcg ggacggctgc
      721 gggctgatgt cgatgacgct cccggtgcgg agtcaattcg gggagcacac ccgattcgac
      781 caggtcctcg aggcggtcgc cagatcccgc cgcgcggcct cggaccggca gttcgacttc
      841 tcctgggagc gggatgtcga aggcgccgga cgcgacggcg agccagcctt ctttcctttg
      901 tcgttcgagt tccatgcgct gccgcccgcc agggtcgtcg ggccggtcac gttctcagtt
      961 gcccgcagct tcgcgtgcac ggatcgcttt gccgtcaagg tcaactgcgt gcagcgcggc
     1021 gacgccgtcg gcatcgagtg gcacttcgat cccaacgtgc tgtcgagccg gcagatcgag
     1081 cacctggccc ggcactacca ggcactcgtc aggagcgcca tcgacaaccc gcgggcgctg
     1141 gttctcgatc tggatctgtt cgacgagacg ctgcggcagg agatcgtcgt cggattcaac
     1201 ggcgtcagaa gcgacgagcc gatcgatcgc tcggtccacg aatgcttcga ggaggtcgcc
     1261 agccagagtc cggaccggac ggcgctcgtc tacgaagatc aactactgag ctacggtgag
     1321 ctcaactccc ggagcaatcg actcgggcgc tacctgcgct cgctgggcgt cggttctgac
     1381 gtgccggtcg cgctgtgtgt cgagcgttcg ctcgacatga tcgtaggcat gctgggagtc
     1441 ttcaaggccg gcggcgcgta cgtgcccctg gaccccagcc tgcccgccga acggctggcc
     1501 ctgctgctcg ccgagtcgcg cgcaccggtc gtcgtctgcc agccgcatct cgcggatctg
     1561 ctttcggggt gtgcggcgaa actggttgtc ctggacgccg ggacgcagcg cgcgatctcg
     1621 caggagaaag ccgagaactg cctcagcggc gtctcgccgg cgaacctggc ctacgccatc
     1681 ttcacctcgg gatcgaccgg cactcccaag ggagtgctcg tcgagcaccg gcagctcgtc
     1741 aactacgtgc acggcgtccg tgaacggctc cggcagccgg gcgacgtcag ttttgcaacc
     1801 gtctccagtt acgcagccga ccttggcaac accgcgatct ttccggcgct gctcggtggg
     1861 ggctgcctgc acgttgttcc ccaggatcgc gtcctggatc ctggcgcaat ggccgactac
     1921 ggcgagcgcc acgccatcga ctgcctgaag atcgtcccca gccatctgtc tgccctgctg
     1981 tccgctccgc gcccggcgcg gctgcttccg cgccgctgtc ttgtggtcgg gggcgaagcc
     2041 tcgacgtggg atctcgtgag ccggttggag tcgctctcgg acgattgcca gatcgtgaac
     2101 cactacggtc cgacggaagc caccgtcggc gtcctcgcct atcgccacga gcgagggcac
     2161 atccgaagag acgagagcga cacgcttcca ctcggccggc cgttgagcaa ctcgcgggtg
     2221 tacctgctgg attctcgggc gcgtccggtc ccggtcggcg tcaacggcga gttgcacatc
     2281 gcaggcgccg gtctcgcgag aggctacctc gatcgcccgg atcagacagc cgagcgcttc
     2341 atccccgatc cgattgccgc ggagccgggg gcccggatgt acagaaccgg agacctggcg
     2401 cgccatctcg ccgatggcaa cgtcgagttc gtcggccgcg tcgaccatca ggtcaagatt
     2461 cgcggtttcc gcgtcgagtt gggggagatc gagtcggcgc tcgagcggca cgaggcgatc
     2521 gacgacgtcg tcgtgacagt atccgagagc ccgtcgggcg acaagcggct ggtcggctac
     2581 gcggttcgcc gacgcggtgc ccaggtcacg accgacgaat tacgagactg ggtccgcgca
     2641 cagctccccg actacatggt gcccggcgcg atcgtctggc tggacgcact gcctctcaat
     2701 cgcaacggga aggtggaccg caaggccttg ccggccgtcg agcacgtcac ggagcggggg
     2761 ccgggatccc ctgtgtcgcc gcgcaccctc acggaagaaa cgctgctggg gatctggtgc
     2821 gccgtgttgg gaaagaccgg cctcggcatc cacgacgact tcttcgagtg tggcggccac
     2881 tcgctgctgg ccatgcagct gatcacgcgc gtgcgcgaag cgtttcgcgt cgatctgcct
     2941 ttgcccgcgt tgttcgacac gccaaccgtg gcgggaatcg cggagtcgat cgagacgctc
     3001 atcggcgccg gaacgtccgg agtggcctcc gcgctgaagc cggtgtcacg cgaccggacg
     3061 ctcccgttgt cgttcgccca gcagcgcctg tggttcctgg aacagctcga gcccaacagc
     3121 agcgcctaca accggccgtt cgcgtttcgg ctgacgggtc cgctcaacct ggaatcactg
     3181 ggacagtcac tggaagaaat agttcgccgg cacgaggtcc tgagaacgac gtacccgacc
     3241 gtggacggcg aagcgcggca ggtgatcgct ccgtacgccg ggttacgcgt gcgtctaatc
     3301 gacctccgtg gcgtgcccct gcaggaacga gatctcgtgg ctcatcgtct ggcagccgag
     3361 gatgctctcc ggctcttcga tctggcccgc ggtccactcg ttcgcgcgtg cgtcatgcag
     3421 ctcaacgacc tggaccatgt cctgctgctg tcccagcacc acatcaacac cgacgcatgg
     3481 tcgaacgatg tgctcgtgca cgaggtatcg aaggcattcg gggccttctc gaacggcacg
     3541 ccttcgccgt tggccgagct gccgattcag tacgccgatt acgcggcctg gcagcgcgaa
     3601 tggctcgaga gcgagcctgc ccggcagcag ctcgtcttct ggacggagga attcaaggct
     3661 gtcccgcccg ccctcgagct gccaaccgac cgtccgcgcc ccgcggcgca gacctttcac
     3721 ggagccgcgc ggcgattccg cctctcgagg cgccttgcgg cttcactgag ggagctcagt
     3781 gggaaggagg ggacgacgct tttcatgacg ctgctggcgg cgttccagac cctgctgcac
     3841 cgctatacgg gacagccgga catcctggtc ggttcgccga ttgccgggcg cacacgggcc
     3901 gaagtggaag ggctgatcgg gttcttcgtc aacacgctgg tccttcgcgc cgacatgcgc
     3961 ggcaaccccc ggttcagcga cgtcctgacg caggtccgtc atcgcgcagt cgcggcctac
     4021 gagaatcagg acctgccgtt cgaacggctg gtcgatgcgc tggatctgga gcgcgacctg
     4081 agccgcacgc cgttgttcca ggtgatgttc gtgctgcaga ccgacgcgct gaagtcgctg
     4141 catctcgcgg acctggtgat cacccaggcc gacgcgccga tcgaggccgc aaagttcgat
     4201 ctgaccctct atctgtctga gaccgagcag ggtctgaacg gatcgctgga gtacaacacc
     4261 gatctcttca acgcatcgac gatcgaccgg atgtggagcc acttcgagac gctcctcgag
     4321 ggaatcgttg cagacccgcg gcgtccggtg ttggagctgc cgctgctggg cgacgcggag
     4381 cggcgccagc tgttggtgga gtggaacgac acacgggctg attatccgca gggcgaatgc
     4441 ctgcacgagg tgatcgagga gcaggtccga cgatctccag acgcggtggc cgtggtcttc
     4501 gaagatcggc agctgacgta cggcgagctg aatcggaagg ccaatcagct ggctcgccac
     4561 ctgcggtcgc tcggtgtggg gccggaagcg cgggttggcc tgtgcctcga tcgctcgctc
     4621 gagatggtgg tgggcgtgtt gggcgtgctc aaggcagggg cggcctacgt gcccatggac
     4681 cctgcgtatc ccaaaggccg gttggcgttc atccttcagg acagccgggc gacggtgttg
     4741 ctggccgacg agcggtcggc cgctgtgctg cccgatcata ccgctcgcgt cgtttccctg
     4801 aaccccgggc tgcagatctg cgccagggag aacggcgaga acctgggccc gaacggagac
     4861 agccgaaacc tttcgcacgt tatctacacc tccggttcca cgggacaacc caaaggtgtg
     4921 gcaatcgaac agcgcagcgt gaccacgctc ctgcgttggt cgcaggataa ctactcgtcc
     4981 gaagaactgg cgggcgtgct cgcctccacg tcgatttgct tcgacctgtc cgtgtgggag
     5041 ttgttcgtgc cgctgagtgt cggtggcgcc gtggtgctcg cacccacggc cctggagctg
     5101 ccaggcctgc tggccgctca gcgcgtgacg ctcgtcaaca cggtcccgtc cgcgatcgcc
     5161 gagcttctgc gaaccggcgg catcccgtcc tcggtccgaa ccgtgaacct cgccggtgag
     5221 ccgctgacga gcgagctgtc cgacgcactt tattcaacgc gcacgattga gaaggtgtgc
     5281 gacctgtacg gtccgtcgga agatacaacc tactcgacct ttgcacgacg aagcctcggc
     5341 ggtcgggcca cgattggtcg gccgctggct gaaacgcgtg tctatctggg cgaggcgggc
     5401 cgcgcgccag tcccgatcgg gatcgcgggc gaactccacc tgggtggcgc ggggctggcc
     5461 cgcggctatc tcgaccgtcc cgacctgact gccgagaggt tcctgcctga cgcgctcggt
     5521 gcggagccgg gccggcggat ctatcgcacg ggcgacatgg cgcgttggcg tgacgacggg
     5581 agcctcgact tcctgggccg catcgatcac caggtcaaga tccggggatt ccgtatcgag
     5641 cttggcgaga ttgagtcggc gctgcgcgga cattccgccg tgcgagacgg catcgtgttg
     5701 gcacgcgagg atcaggccgg cgtcaagcgg ctggtcgcct atctgaccgc cgagggtccg
     5761 ccgccaacgg tcaccgaact gcgcgaacac cttcgggatc ggctcccggg ctacatgatc
     5821 ccggcggcct tcgtggtgct ggacgccctg ccgctcctgc ccaacggaaa gatcgatcgc
     5881 cgcgcgctgc cggcgccgga acagacccgt cctgaactgg aggaagcctt cgtcccggcc
     5941 cgcacgccga tcgaacaacg cctggctggg atctggtgcc gggtgcttgg cctggagcgg
     6001 gtgggcgtgc acgacaactt cttcgagttg ggcggtgatt cgatcctgag tatccagatc
     6061 gtgtcgcgcg ccaaccagga cggcctgcgc ctgagcgcga aacaagtctt cgagcatcag
     6121 acgatcgccg agctggcggc cgtggccggc acggctccgg ccgttcaggc cgagcaaggg
     6181 cgcgtgacgg gtggcgcgcc cgtcacagcg atccagcaat ggtttttcga tcagcagttc
     6241 gcggagcccc atcacttcaa ccaggccgtg ctgctcgaca tcaacgaggc actcgatccc
     6301 gacattcttc ggactgccgt tgacgcctta ctggaacagc acgacgcgct ccggctgcgc
     6361 ttcgtggaac gggatggcgg ttggcgccag gaggatacgg ctgcctcctc gacgacggtc
     6421 tcgcgcatcg atctgtccca tctgaacgac gtcgaacagc ggacggcact cgaggcagaa
     6481 accgccagga tccaggcgag tctgactctt cacgcgggtc cacagatccg agtggcgctc
     6541 ttcgaccttg gtgcgcgagg acggcgtctg ctcctggtga ttca
//
LOCUS       DBNGSH010000003        15239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4075453, whole genome shotgun sequence.
ACCESSION   DBNGSH010000003 DBNGSH010000000
VERSION     DBNGSH010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 15239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15239
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4075453"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="AC16CY_00020"
     CDS             complement(<1..1133)
                     /locus_tag="AC16CY_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3352186.1"
                     /translation="MRRLLQDWVAEQADKRPTSTAVVCAGARLTYAELEALSNQVARQ
                     LRESGCQRGERVALLMPKSTTAIASLLGIYKADAVYVPLDPSSPASRLRKILDSCECR
                     WLLAAGAVTPTIEELLQTGQGRHPLCVGWLEPAPPSRAVFDVRFTLDDVVGLPAESFA
                     RYNRPEDPAHILFTSGSTGTPKGVVITHDSLIRLVEWATAHFRLEASDRLSGHPPLHF
                     DMSFLDIFSAAAVGAELHLVSRELSLLPNNLAEFIRTSELTQWFSVPSVLNYVAQFDL
                     IAPGDFPSLRRVMWAGDVLPTPALTYWMRRLPHVSFTNLYGPTETTVVSSHYTVPACP
                     EDPTAPIPIGTACDGEELLVLDESMHPPPAGTPGDLYIGGVGLA"
     gene            complement(1130..2137)
                     /locus_tag="AC16CY_00025"
     CDS             complement(1130..2137)
                     /locus_tag="AC16CY_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3352187.1"
                     /translation="MTPESSIEVFSTCPPSTGVPQELYLERFTEIARWSEEAGCRGIL
                     LFSDNSVLDPWMLAHLIFQRTRTLCPLIAVQPVYMHPYWVAKQIASLAYLYGRRLYLN
                     MVAGGFKNDLEALDDTTPHDKRYARLIEYTLVIMGLLSSRAPLSFDGKFHRVSKLKLA
                     PSLPEALLPGVFVSGSSEAGITAARVLGATAIKYPKPPEEEEERPAGLVNPGIRVGII
                     ARRTDKEAWDVAQERFPEDRKGQLTRQLAEKVSDSVWHKQLSGLEAIDHTGPYWLTPF
                     QNYKAMCPYLVGSYETVGAELGRYFASGYRTVILDIPPSLDDLRHSFTAISAALEPVQ
                     S"
     gene            complement(2152..2766)
                     /locus_tag="AC16CY_00030"
     CDS             complement(2152..2766)
                     /locus_tag="AC16CY_00030"
                     /inference="COORDINATES: protein motif:HMM:NF041523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-COAP-1 domain-containing protein"
                     /protein_id="HIR3352188.1"
                     /translation="MRTSIITAVAAVALVPLGLALANRSVMAHPAPCDFMTGGGFIFT
                     TASGTHDLAKANFGVGGGCKHGSPTWGHLEYIDHGTKLNVHWQTITAYLAEGDGLGDT
                     STDPKTGQPTGTRLICGTARTNQLGDVDFMVRARDAGEPGVNDEFDIRLSKLGLIVYS
                     TENEPDMPHKLGGGDGGGGNIQLHKPNPSTAGQLGGDCPARDAI"
     gene            complement(2847..3455)
                     /locus_tag="AC16CY_00035"
     CDS             complement(2847..3455)
                     /locus_tag="AC16CY_00035"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HIR3352189.1"
                     /translation="MHTNSLYRWSLLALGLAAAGLGWPAHGSAQANTASGHARAVQAT
                     VAAPSGVDTTVLTDTGTLGSLDDAREASQNTGSIGSWLGGEALHATSIGRPDQVESEA
                     SLANLVLNVGGATVTADFVMSRAQAARGSRSGVVEVQGLVVNGTPIVVTGDGDQSVPF
                     PGGSVVINESRRSQGGIVVNALHVIVDGAADVVVASASATYR"
     gene            complement(3526..4275)
                     /locus_tag="AC16CY_00040"
     CDS             complement(3526..4275)
                     /locus_tag="AC16CY_00040"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR3352190.1"
                     /translation="MNAIATATDMSGLAVADAGPNLGEELVSFAAPGSAEADRYRILR
                     QAVERFGRDTGARVFAVTSPGPGEGKTLTTLNLAGSLARAPEARLLVVDADLHRPSVA
                     RYLGLALSGAPGLTEALLYEDLTLARTARRIDSLGISILHAGRTRRVPYELLASPRFE
                     GLVGDMRRLYDYVLIDTPPVVPIADCQLLARWVDAFLVVVAAHRTPRRALAEALSVLG
                     PKVLGIVFNGDDRPLGKYYGYDSYTNPRAAR"
     gene            complement(4272..5855)
                     /locus_tag="AC16CY_00045"
     CDS             complement(4272..5855)
                     /locus_tag="AC16CY_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HIR3352191.1"
                     /translation="MSRDFDQPNGHDSAQYAPDFFLDVWRRRKWIAAAVFAAVLAGAL
                     SVAASLPDLYRATAKVIVDRQEVSEAFVQPSVTAELETRIQTIDQRVRSREILGGVIT
                     SMNLYPELRQRATLDSVVERMRRDMVLRLEGVTQASGRNATIAFTLSYRGGDPDTVSS
                     VANALAVLYVEENTKSRESQAMKTAGFLEQEVSNLKRELNAQESKAGDFAQSNAFELP
                     QQLEVNMAAVERLTNDLRRIGDDRLRILERRDRLERELAETEAPDGASSEETVATRLN
                     ALRRERTELRRQYSDLYPEVIRVNAEIAALEAQVRSGESGDAAASRPAGKQRANPALA
                     EVDAQLAALTREETKLKQQIRTYEARIEATPRRQREMEQLSRGTAAERERLEKLVQKY
                     EDARRATNLELGQDVEQFRILDPAIPPRRPVAPDRLWLVAMGLAAALALAVAAVVVAE
                     KLDMTFHSSDDVRAFTGLPVLATIRQVPTAASARRQRLQVAAGMCVAVLGLSLIAAGS
                     YYVAAGNEDVVRLIARGDA"
     gene            complement(5855..6475)
                     /locus_tag="AC16CY_00050"
     CDS             complement(5855..6475)
                     /locus_tag="AC16CY_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR3352192.1"
                     /translation="MTTLKALTLGIALLPVAAGPCVAGQRAPAGQARPAAEAGHPDPG
                     DYLIGPDDVLEIAVWNNEKITRTVPVRPDGKISLPLLNDVQAAGLSAMQLRAFLAKAL
                     AAFVQTPEVSVIVREVHSFKVSVMGEVKEPGRFDLTARATVLDVLAMAKGFTEFADKG
                     RIVVLRREKGLTRLIPFEYHKIMSGGESNSGRENFFVQPDDIVLVR"
     gene            complement(6472..7629)
                     /locus_tag="AC16CY_00055"
     CDS             complement(6472..7629)
                     /locus_tag="AC16CY_00055"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3352193.1"
                     /translation="MDLTSALRRATSRVAARQRLERVLILGTGLLAEQVVLEIDNRPG
                     CRAVVLGVLDDRAPATDGPTGRLFLGSVDGLAAVIDERRPNRILVALAERRNRMPMQA
                     LLESSIARGITVEDAADYYERLTGKLALESLSPMSVVFSGRLRPSQLQQTLMRIVSLA
                     SALVALVVLFPLLVTIALAIKLDSPGPVLFVQRRVGAYGRPFMLRKFRTMHDGRKRRS
                     EWEGDNRDQVTRVGRWLRAFRLDELPQFLNILRGEMNLVGPRPHPVSNLELFTLVARN
                     LNELTGAAVSCYMLRTLVRPGLTGWAQVRYRYANNLEEEIEKLRYDLYYIKHMSPWLD
                     FRILLETMKALFGHGLACLGSRRGETVESSRNVETAAPSGMRGKIGRTREA"
     gene            complement(7604..8779)
                     /locus_tag="AC16CY_00060"
     CDS             complement(7604..8779)
                     /locus_tag="AC16CY_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352194.1"
                     /translation="MTLIGLRALVGRAARAALVSASLLVPATAGAQGQSFVRVTGSFE
                     QLYDDNLFAAPLTAPQQPGLISRFGPAVEAGYRSIPVKLVVRYGFDAERHITLPELDE
                     AIARQDALVDFAHQTKRLTLTVGTSYQETQAPRELNQQSLLAVGRARAQRITARPGLV
                     YELTALTKVKLDHNFSADALAGGVSSATHVSRSGIDRRLDARNVVRFDYRFRNVAFSD
                     GLEERSHLATAGWTFEIAPSTSVEIDAGPRVTRGSGGVEPEVSLVLKHRLRQGEFSFS
                     YARTLDTAIGESGTIDVHRLGTVVSFVPTRYFTLTASPAWVSSARDVRRTVVHALDLE
                     GRVRATRRWSLLASGRLGGQEGTIPGRTDVIQYRSASLKSIVTLGTLERDGSDVGTP"
     gene            complement(8795..9364)
                     /gene="nusG"
                     /locus_tag="AC16CY_00065"
     CDS             complement(8795..9364)
                     /gene="nusG"
                     /locus_tag="AC16CY_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR3352195.1"
                     /translation="MPHITITASDGEQNPGLADAGQGSRCWHALWTHSHCEQLVHDQL
                     AHKGVQAFLPKVDVWSRRRGVRHLVSVPMFPGYLFFRQAIDQTSYGDVSRTRGVVRVL
                     GERWDRPAEIPEEEIAAIAKVVTERHPVLPFPYLREGQRVRVSTGPLAGVEGILVRAK
                     PDKGLLVLSVHLLQRSVSMSIDATAVTPA"
     gene            complement(9623..9979)
                     /locus_tag="AC16CY_00070"
     CDS             complement(9623..9979)
                     /locus_tag="AC16CY_00070"
                     /inference="COORDINATES: protein motif:HMM:NF020257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase RsbU N-terminal domain-containing
                     protein"
                     /protein_id="HIR3352196.1"
                     /translation="MSAHKALFARRYRAALLDYLLGSDETGLVRAYDLGRLALEGGLG
                     PLEILRTHQRAAQAILASTQSADLTRKRVAAAGEFLMETLSPFEMTYRGYVALIEARH
                     HGLGESHSRRRVNRKK"
     gene            complement(10109..10735)
                     /locus_tag="AC16CY_00075"
     CDS             complement(10109..10735)
                     /locus_tag="AC16CY_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3352197.1"
                     /translation="MRVVLADDHPVVRQGLRMLLERERFEVVGEASDGLEAIGLAEAH
                     HPDVVVLDLAMPGLNGISAARDIARVAPRAKIILLTMYTEEHHVLEALRAGVKGCVSK
                     TQAAEHLLQAIREVSAGGVYLSPSVSGAVVQAYLSNSEPPADPLTNRERQVLQLVAEG
                     KTTKETAVILGVSVKTAETHRTKLMEKLDIHSTAGLVRYAIRRGLVQP"
     gene            complement(10744..11667)
                     /locus_tag="AC16CY_00080"
     CDS             complement(10744..11667)
                     /locus_tag="AC16CY_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3352198.1"
                     /translation="MSDRLCTLSDDYSMALRQYVSNQDEAALARAYELGRKALEDGLG
                     VLDVATVHSRALPALLLRPLHEHEHAKLLEALEKFLVEALSPFEMAFLGFRESNVVLH
                     RVNDVLEGQARRIGSALHDEAAQLLASVHLALADLAVRQPGTAKPVQGVRGLLEQIED
                     RLRNLSHELRPPILDDLGLVPALEFLADTISKRWGVGVAVHATLDRSLPATVETTLYR
                     IAQEALNNVVKHAKATQASVCLRRESHKVACSILDDGVGFDAAAVARRSGARGLGLVE
                     IQERVVALGGALRLGDNDGGGTDLTVEIPLE"
     gene            complement(11664..12320)
                     /locus_tag="AC16CY_00085"
     CDS             complement(11664..12320)
                     /locus_tag="AC16CY_00085"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3352199.1"
                     /translation="MGSVTMPQHHGRTVCPGGVVAWGVAEMPLPAEPESGDLFVVETR
                     SDGVLIAVIDGVGHGSDAAAAAKAAAAVLRAHVSDSPVSLVLRCHDALKRSRGVTMTV
                     ALLDSCDRTLTWLGVGNVEGVLVHEDGTERESPERALLRGGVVGYRLPPRLRAEVVTL
                     KPLDTLIVATDGIRPDFADGIALNGDPEGIAAGIMARHRSGVDDALVMVARYLGGEAP
                     "
     gene            complement(12308..12709)
                     /locus_tag="AC16CY_00090"
     CDS             complement(12308..12709)
                     /locus_tag="AC16CY_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HIR3352200.1"
                     /translation="MEPEVRVRIQSSTDIVIARQQGRALAAQAGFSNSNLTIIATAIS
                     EVARNIVEYAKEGEVVLTLLNNAGSAGIKIVALDHGPGIADIATVMRDGYSTGKGLGI
                     GLPGSRRLMDEFEIASEVGKGTTVTMKKWAV"
     gene            complement(12699..13067)
                     /locus_tag="AC16CY_00095"
     CDS             complement(12699..13067)
                     /locus_tag="AC16CY_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3352201.1"
                     /translation="MAVPILKQSHFLIASIQSALSDQDLVQLRDDLADLVGRHRARGV
                     IVDVTALDVLDSFATRTLRAIAYTTKLRGAETVIVGIQPEVAMSMVQLGLTLENVPTA
                     LDLEEGLLFLKLRLEDGHGA"
     gene            complement(13181..14053)
                     /locus_tag="AC16CY_00100"
     CDS             complement(13181..14053)
                     /locus_tag="AC16CY_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3352202.1"
                     /translation="MTSSNRESTTALLRELVSHLRENRTELREEWARRITESRLLTAM
                     TREEIFAEATTVYDNYVAVLETGSVEALQAYARDLSERIIPRGVETQEVLGIVLLLRD
                     VLARSLFKKYQLDFKLLNFVLDAYEPAANRIANTVAVGFVQERERIIRQQQEAIRELS
                     TPVLRVRERLLILPIIGLIDPQRARQLTEQLLRGIRENRGKVAVMDITGVPVIDSAIA
                     NRLVQTVEAARLLGATVIVTGLSPEIAQTLVTIGVDLSKMNTVGDLQGGIELAERLLG
                     FKAVALEEIPPRVQ"
     gene            14314..>15239
                     /locus_tag="AC16CY_00105"
     CDS             14314..>15239
                     /locus_tag="AC16CY_00105"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3352203.1"
                     /translation="MIRRHLGGILVAVALGIAAAAAQTQTQKPNPQNQATEDFEAPAA
                     FPLAAPKGTDSRAMTEAPPKAVNTGPFDPATWKYGTAFAPPPGTKIWNPVKLKMLQGG
                     KVTGGTLFSATDPATYCAMADAGYDFIWTEMQHDQRDWDQAAQMWRTCPYARAVPGVR
                     VARAEEREIQHALDAGALVVAVPTVDTVEEAIEARNWTYFPPLGRRSAGGGQAFEQDM
                     WGRVPGGYRNTANDNIVLILMIETLEGLKNAEAIAKVPGVTALFAASGDLGNFTGFRQ
                     GSPDYERVINIVHDAAIKAGVRLCGPLAWRDRP"
BASE COUNT         2567 a         5063 c         5068 g         2541 t
ORIGIN      
        1 gcgagcccga ccccgccgat gtacaagtct cctggcgtgc cggctggagg cgggtgcatc
       61 gattcgtcca gcacgagcag ctcttccccg tcgcacgccg ttccaatcgg gattggcgcg
      121 gtcggatcct cgggacacgc ggggacggtg tagtggctgc tgacgacggt cgtctcggtc
      181 gggccgtaga ggttggtgaa gctgacgtgc ggcagtcgcc tcatccagta cgtgagcgcg
      241 ggcgtgggaa ggacgtcgcc cgcccacatg acgcgcctca acgaggggaa atcgccgggg
      301 gcgatgagat cgaactgcgc cacgtagttc aggaccgagg gaacggaaaa ccactgggtg
      361 agctccgacg tccggatgaa ttcggccagg ttgttcggca gcaggctgag ctctcgcgac
      421 accaggtgga gctcggcgcc aacggcagcc gcgctgaaga tgtcgaggaa cgacatgtcg
      481 aagtggagcg gcggatgccc cgacaggcga tccgaggcct ccaggcggaa gtgcgccgtc
      541 gcccactcga cgagccggat caggctgtca tgcgtgatca cgacgccctt gggcgtgccc
      601 gtggagccgg atgtgaagag gatgtgcgca ggatcttcgg ggcggttgta gcgtgcgaac
      661 gactcggccg gaagaccgac gacatcgtcg agcgtgaagc gaacgtcgaa gacggcacgg
      721 cttggcggcg caggctccag ccagcccacg cacaacgggt ggcgaccctg ccctgtctgg
      781 agaagctcct cgatggtcgg cgtcacggcg cccgccgcga gaagccatcg gcactcgcag
      841 gaatccagaa tctttcgcag ccggctggcg gggctcgacg ggtcgagcgg cacgtagacg
      901 gcgtcggcct tgtagatgcc gaggagcgac gcgatggccg tcgtcgattt cggcatcagc
      961 agggcgacgc gctcgccgcg ttgacatccg ctctccctga gttgcctggc aacctggttc
     1021 gacagagcct ccaactcggc gtaggtgaga cgcgcgccgg cacacacgac ggcagtcgac
     1081 gtggggcgct tgtccgcctg ctcggccacc cagtcctgga ggagtcgtct catgactgca
     1141 ccggctccag ggccgcactg attgcggtaa agctgtgccg cagatcgtcg aggctcggcg
     1201 ggatatccag gatgacggtg cgatagccgg aggcgaagta gcggccgagc tcggccccga
     1261 cggtctcgta gctgccgacc agatacggac acatcgcctt gtagttctgg aacggggtca
     1321 gccaataggg ccccgtgtgg tcaatggcct cgagtccgga caattgcttg tgccacaccg
     1381 aatcggaaac cttctccgcc agctgacgcg tcagctggcc tttccgatcc tcggggaagc
     1441 gttcctgggc cacgtcccaa gcctctttgt cagttcgccg ggcaatgatg cccaccctga
     1501 tgccagggtt gacgagacct gcaggccgtt cttcctcttc ctctggcggc ttcggatact
     1561 tgatggccgt cgcgccgaga acccgtgccg ccgtgatccc ggcctccgac gagcccgaga
     1621 cgaagacgcc gggcaggagc gcctccggca acgacggcgc cagcttcaac ttcgacacgc
     1681 gatggaactt gccgtcgaag ctgagtggcg cccggctcga cagcagaccc atgatgacca
     1741 gcgtgtactc gatcagccgc gcgtagcgct tgtcgtgcgg cgtggtgtcg tcgagcgcct
     1801 cgagatcgtt cttgaagcct cctgccacca tgttgagata gaggcggcgg ccgtagagat
     1861 acgcgagcga ggcgatctgt ttcgcgaccc agtacggatg catgtacacg ggctgtacgg
     1921 cgatcagggg gcacagcgtc ctcgtgcgct gaaagatgag atgcgcgagc atccacgggt
     1981 cgagcacgct gttgtcggag aacagaagga tgccccgaca ccccgcctcc tcgctccatc
     2041 gagcgatctc cgtgaagcgc tcgagataga gctcctgggg tacgccggtc gaaggcggac
     2101 aggttgagaa cacctcaatc gacgattcgg gtgtcatggc atcctcctca ttcagatcgc
     2161 gtctctggcc gggcagtctc caccgagctg gccggcggtc gatggattcg gtttgtggag
     2221 ctgaatgttg ccgcctccac cgtcgcctcc gccgagctta tggggcatgt cgggctcgtt
     2281 ctcagtcgag tagacgatga gaccgagctt ggacagccgg atatcgaact cgtcgttcac
     2341 tcccggctcg cccgcgtccc tggccctcac catgaagtcg acgtctccca actggttcgt
     2401 tctcgccgtg ccgcagatca gccgggttcc cgtcggctgg ccggtttttg gatcggtgga
     2461 cgtatctccg aggccgtcgc cttcggccag atacgccgtg atggtctgcc agtgcacgtt
     2521 caacttcgtg ccgtgatcga tgtactcgag atgaccccac gtcggcgacc cgtgcttgca
     2581 gccgccgcca acaccgaagt tggccttcgc aaggtcgtgc gtgccgctgg cggtcgtgaa
     2641 gatgaatcca ccgcccgtca tgaagtcgca tggcgccgga tgcgccatga ccgaccgatt
     2701 ggccagcgcg agcccgaggg ggacgagagc aaccgcagcg actgcggtga tgatcgaggt
     2761 gcgcatttga agattcctcc gcttcgaagt gggagcggtg cgagcgccgt tccctgaatg
     2821 tcatgtgagc ccatggatcc tgttggtcag cggtatgtcg ccgaagccga cgccacgacc
     2881 acgtccgcgg cgccatcgac gatcacgtgg agggcgttca cgacgatgcc gccctgcgac
     2941 cgccggcttt cgttgatcac gaccgatccc cctggaaagg gcacgctctg gtctccgtcc
     3001 ccggtcacca cgatcggcgt cccgttgacg acgaggccct gaacctcgac gacgccggag
     3061 cgactgcctc ttgccgcctg ggctcgtgac atgacgaagt ccgcagtgac cgtcgcgcct
     3121 ccgacgttga gcaccaggtt cgcgagcgac gcttcggatt cgacctgatc gggccggccg
     3181 atggaggtcg catgaagcgc ctcgcccccg agccacgatc caatcgatcc cgtgttctgc
     3241 gatgcctcgc gcgcatcgtc gaggctccca agcgtcccgg tatcggtcag caccgtggtg
     3301 tccactccgg acggggcggc gacggtggcc tggaccgcac gggcgtgccc gctggccgtg
     3361 ttggcctgcg cggatccgtg agccggccat ccaagcccag cggcggcgag gccaagggca
     3421 agcagactcc agcgatagag cgagttcgtg tgcatgtatc acctccatga agtcccgcct
     3481 gtccacttcg gatgggcggg gggctgaaca aggggcgcgg ctgccttacc gggcagcgcg
     3541 gggattcgtg tagctgtcgt acccgtagta cttgccgaga ggccgatcgt cgccgttgaa
     3601 cacgatgccc aggaccttgg gccccagaac gctcagcgcc tcggcgagcg ctcgtcgagg
     3661 cgttctgtgg gctgccacga cgacgagaaa ggcatcgacc cagcgtgcga gcagttggca
     3721 gtctgcgatg gggacgacgg gcggcgtgtc gatcagcacg tagtcgtaca ggcggcgcat
     3781 gtcgccgaca agcccttcga acctgggaga cgccagcagc tcgtacggca cgcgtctggt
     3841 ccggccggcg tgcagaatcg agatgccgag cgagtcgatg cgtcgcgcgg tccgagcgag
     3901 ggtcaagtcc tcgtagagga gggcttcggt gagtcctgga gcgcccgaca gcgcgagccc
     3961 gaggtagcgc gccaccgaag gccgatgaag gtcggcatcg acgacgagga gcctggcctc
     4021 gggcgcgcgg gcgagggatc ccgccaggtt cagggtggtg agggtcttgc cctcgcccgg
     4081 ccccgggctc gtgacggcga acacgcgcgc gcctgtatcg cgcccgaatc gctccacggc
     4141 ctggcgaagg atccggtacc gatcggcttc ggcggagccg ggcgcggcga agctgacgag
     4201 ttcctcgccc aggttggggc ccgcgtcagc gacggccagc ccgctcatgt cggtcgcggt
     4261 tgcaatggcg ttcacgcgtc accccgcgca atcagccgca ccacgtcctc gttgccggcg
     4321 gcgacgtagt acgagccggc cgcgatgagc gagagtccga ggacggccac acacatgccg
     4381 gcggcaacct gcaaccgttg tcggcgcgcg ctggccgccg tcggcacctg gcggatcgtc
     4441 gccagcacgg gcaggccggt aaacgcgcgc acgtcgtcgg aggaatggaa cgtcatgtcg
     4501 agcttttcgg caacgacgac ggcagcgacc gccaaggcga gcgcggccgc gagtcccatc
     4561 gcgacgagcc acagcctgtc gggcgccacg ggcctgcgcg gcgggatcgc cggatcgaga
     4621 atccggaact gctcgacgtc ctggccgagc tcgagattgg tagccctccg agcgtcctcg
     4681 tatttctgca cgagcttttc gaggcgctcg cgttcggcgg cagtcccccg ggacaactgc
     4741 tccatctcgc gctgtcgccg tggcgtggcc tcgattctgg cctcgtacgt ccggatctgc
     4801 tgcttgagct tcgtttcctc ccgtgtcagc gctgcaagct gagcgtccac ctccgcaagg
     4861 gcgggattcg cgcgctgttt cccagcgggc cggctcgccg cggcgtcgcc actctcccca
     4921 ctgcggacct gcgcctcgag agcggcgatc tcggcattca cccggatgac ctcgggatag
     4981 aggtcgctgt actgtctgcg caactcggtc cgttcccgcc ggagcgcgtt gagacgcgtg
     5041 gcgaccgtct cctccgagga ggcaccgtcc ggcgcctcgg tctcggccag ctccctttcg
     5101 aggcggtcgc gccgctcgag gatgcgcagg cggtcgtcgc cgatccggcg gaggtcgttg
     5161 gtcaggcgtt cgacggccgc catgttgacc tcgagctgct gcggcagctc gaaggcgttg
     5221 ctctgggcga aatcgccggc tttgctctcc tgggcgttca gctcgcgttt gaggttggag
     5281 acctcctgct cgagaaatcc tgctgtcttc atcgcctgac tctcgcgaga tttggtgttc
     5341 tcctcgacat agagcacggc gagggcgttg gcgacactcg agacggtgtc gggatccccg
     5401 ccgcgatagc tcagggtgaa ggcgatcgtc gcgttgcgcc cgctggcctg cgtcacaccc
     5461 tcgagccgca gcaccatgtc gcgacgcatg cgctcgacga ccgagtcgag ggtcgctctc
     5521 tgacggagct ccgggtacag gttcatgctc gtaatcaccc cgccgaggat ctcccggctc
     5581 ctcacacgct ggtcgatcgt ctgaatcctc gtttcgagct cggcggtcac ggatggttgg
     5641 acaaatgcct ccgagacctc ctggcgatcg acgatcacct tggccgtggc gcggtacaga
     5701 tcgggaagcg acgccgccac gctgagcgca ccagccaaca cggcggcgaa caccgcggcc
     5761 gcaatccact ttcgacgcct ccacacatcg agaaagaagt ctggggcgta ttgggcgctg
     5821 tcgtggccat tcggctgatc gaaatctcgg ctcatcaccg caccaggaca atgtcgtccg
     5881 gctggacgaa gaagttctcg cggccgctgt tcgattcgcc gccggacatg atcttgtggt
     5941 actcgaacgg gatcaggcgg gtaaggccct tctcccgcct caggacgacg atgcgtccct
     6001 tgtcggcgaa ctcggtaaag cctttggcca tcgccagcac gtcgagcacc gtggcgcgcg
     6061 cggtcaggtc gaaccggcct ggttccttca cctcacccat gacggacacc ttgaagctgt
     6121 gcacctcgcg gacgatgacg gagacctcgg gggtctggac gaaggcggcc agcgcctttg
     6181 cgagaaacgc gcgcaactgc atggccgaga ggccggccgc ctgcacgtcg ttgagcaacg
     6241 ggagggagat cttgccgtcc ggtcgaacgg gaaccgttcg tgtgatcttc tcgttgttcc
     6301 agaccgcgat ctccaggacg tcgtctggtc cgatcaggta gtcgccgggg tccgggtggc
     6361 cggcctcggc cgcgggcctc gcctggccgg cgggagcccg ttgccccgcg acgcagggcc
     6421 cagccgcaac cggcagcagg gcgatcccga gcgtcagcgc cttcagcgtc gtcatgcttc
     6481 ccgtgtccga ccgatcttgc cgcgcatgcc ggacggcgcg gctgtctcga cgttccggga
     6541 cgactcgacc gtctcgccgc gccggctgcc gaggcaggcc aggccgtgtc cgaacagcgc
     6601 cttcatcgtc tcgagcagga tgcggaagtc gagccatggc gacatgtgct tgatgtagta
     6661 caggtcgtac cggagcttct cgatttcctc ctcgaggttg ttggcgtagc ggtaccgcac
     6721 ctgcgcccac ccggtcaggc cggggcggac gagcgtccgc agcatgtagc agctgaccgc
     6781 cgcgccggtc agctcgttca ggttccgcgc tacgagcgtg aacagctcga ggttcgacac
     6841 cgggtggggc ctgggcccga cgaggttcat ctctccccgc aggatgttga gaaactgggg
     6901 cagctcgtcg agccggaagg ctcgcagcca ccggcccact ctcgtcacct ggtcgcggtt
     6961 gtcgccctcc cattcggagc ggcgcttacg gccgtcgtgc atcgtgcgga acttccgcaa
     7021 catgaacggc cggccgtaag cccccacgcg ccgctgcacg aagagcaccg ggcccggtga
     7081 atccagcttg atggcgagcg cgatcgtgac cagcagcggg aacagcacga cgagcgccac
     7141 gagcgccgac gcgagactca cgatccgcat cagcgtctgc tggagctgcg aggggcggag
     7201 ccgcccggag aacacgacgc tcatgggcga caacgactcc agggcgagct tgcctgtcag
     7261 gcgttcgtag tagtccgcgg cgtcctcgac cgtaatgccg cgcgcgatgg atgactcgag
     7321 gagggcctgc atcggcattc tgtttcgccg ctcggccaac gccacgagaa tccgattcgg
     7381 acgtcgctcg tcgatgaccg cggccagccc gtcgacgctc cccagaaaga gccggccggt
     7441 cggaccgtcc gtcgcggggg cgcgatcgtc gagcacaccc aagacgacgg cccggcagcc
     7501 gggccggttg tcgatctcga gcacgacttg ctcggcgagc agccccgtcc cgagaatcag
     7561 cacgcgctcg agccgctggc gggcggccac gcgggacgtg gccctacgga gtgccgacgt
     7621 cagatccatc gcgctccaat gtgccaagcg tcacgatcga tttgagcgac gcgctgcgat
     7681 actgaatcac atccgtacgt cccgggatgg tgccttcctg tccgccaagg cgccccgagg
     7741 ccaacagcga ccatcgtcgc gtcgcgcgca cgcgcccctc cagatccagc gcatgcacga
     7801 ccgttcgtct cacgtcgcga gcgctggaca cccatgccgg agacgcggtc agcgtgaagt
     7861 agcgcgtcgg aacgaatgag acgaccgtgc cgagccggtg cacgtcgatg gttccggact
     7921 caccgatcgc ggtgtccagc gtccttgcgt acgagaagga gaactcgccc tgtctcagtc
     7981 tgtgtttcag gaccaaggac acttccggtt cgacgccgcc ggagccgcgc gtgacgcggg
     8041 gccctgcgtc gatctcgacg ctggtcgacg gcgcgatctc gaaggtccag ccggcggtcg
     8101 cgagatgaga tcgttcctcg aggccgtcgc tgaaggcgac gtttcgaaag cggtaatcga
     8161 atcgaaccac gttccgcgcg tcgagccgac gatcgatgcc cgatcgcgag acgtgcgtgg
     8221 cgctcgagac gccgcctgcc agtgcgtcgg ctgagaagtt gtggtcgagc ttcaccttag
     8281 tcagggcggt gagctcgtac acgaggccgg gccgcgcggt gatccgctgg gcacgggccc
     8341 gcccgacggc gagcaggctc tgctgattga gctcgcgagg cgcctgcgtt tcctgatacg
     8401 aggtcccgac agtcaacgtc agacgcttgg tctgatgagc aaaatcgacg agggcatcct
     8461 gccgcgcgat ggcctcgtcg agctcgggga gcgtgatgtg tcgttcggcg tcgaacccgt
     8521 atcgcacgac gagcttcacc ggaatcgagc gataccccgc ttcgaccgcg ggaccgaagc
     8581 gcgagatcag gccaggctgc tgaggggccg tcagaggcgc ggcgaacagg ttgtcgtcat
     8641 agagctgctc gaaggatccc gtgaccctga cgaagctctg gccttgggca cccgccgtcg
     8701 cgggcaccag gagagaggcc gacacgagcg ccgcccgtgc ggctcgtccg acaagggcgc
     8761 gaagtccgat gagtgtcatc tgagctggtc gagcctacgc gggcgtgacg gccgtggcgt
     8821 cgatcgacat ggagacgctt cgctgaagca ggtggaccga cagcacgagg agccctttat
     8881 cgggcttcgc gcgaacgaga atgccctcga cgccggcgag ggggccggtg gagacgcgga
     8941 cgcgctgccc ctccctcagg tagggaaacg gaagcacggg gtgccgctcg gtcaccactt
     9001 tggcaatggc ggcgatttct tcctcgggaa tctcggccgg gcggtcccag cgctcgccga
     9061 gcacgcgcac cacgcccctc gtccggctca cgtcgccgta ggaggtctgg tcgatggcct
     9121 ggcggaagaa gagatacccc gggaacatcg gcacgctcac cagatgccgg actccacgcc
     9181 ggcgcgacca gacatccacc ttcggcagga acgcttgcac gcctttgtgc gcgagctgat
     9241 cgtgcacgag ctgctcacag tggctgtggg tccagagcgc atgccagcac cgggaaccct
     9301 gaccggcgtc cgccaggccc ggattctgtt cgccgtccga cgcggtgatc gtgatgtgtg
     9361 gcaaggcttc gcccccttcg gtgcatcctg cgctcctcgc acgggatgcc cgccatgact
     9421 cgatccttac ggggggacca accgccgctc catcgagcgg cgtgtgatcg aaggacaacc
     9481 agatcgcctg ggcgcccaat cgccccggcg acgccctcta tcctcataga ggcggctggg
     9541 ggagggaatc gggtgatccc cgtgaacgac gctgggaaga tcccggtgag agtggaagga
     9601 acttaccgtt aacgccgagc ggctacttct tccggttgac ccgccgacgt gaatgagact
     9661 caccgagccc gtggtgcctg gcttcgatca gcgccacgta gccgcgatag gtcatctcga
     9721 acggcgacag cgtctccatg aggaactctc ctgcggcggc cacacgcttg cgcgtcagat
     9781 ccgcgctctg cgtggacgcg aggatggcct gggccgccct ctggtgcgtg cgcaggatct
     9841 cgaggggtcc caaccctccc tcgagcgcca ggcgtccgag atcgtacgcg cggacgaggc
     9901 ctgtctcgtc gctgccgaga agatagtcga gcagcgccgc gcggtaccgc cgggcaaaga
     9961 gcgctttgtg cgcggacatg ggaaacacga acacccaccg aagccggtta tggcacacga
    10021 cgacgaatca cgccatcggg taaagctcat acacgcaacg cagaagaaac cgggaacgac
    10081 gtcagcgtgg cccgggggga agggccgatc agggctgaac gagcccgcgg cggatcgcgt
    10141 atcgcacgag tcctgccgtc gagtggatgt cgagtttctc catcaacttg gtgcggtgcg
    10201 tctcggccgt cttgacgctg acgcccagaa tgactgcggt ctcctttgtg gttttgccct
    10261 cggcaaccaa ctgcagcacc tggcgctccc ggttggtgag gggatcggcg ggaggttccg
    10321 aattcgacag gtacgcctgc acgaccgcgc ccgagacgct cgggctcaga tagacgccgc
    10381 cagccgacac ctcccgtatg gcctggagca ggtgctcggc tgcctgcgtc ttcgatacgc
    10441 atcctttgac gccggcacga agcgcttcga gaacgtgatg ttcctcggtg tacatcgtca
    10501 gcaggatgat cttggcgcgc ggcgccaccc gcgcgatatc ccgcgcggcg ctgatgccgt
    10561 tcagcccagg catggcgagg tcgagcacga ccacgtcggg gtgatgcgcc tcggccagcc
    10621 cgatcgcctc gagcccgtcc gaggcttcgc cgacgacctc gaacctctcc cgctcgagca
    10681 gcattctcag cccctggcga acgacgggat ggtcatcggc aagcacaact ctcatgtgtc
    10741 cacctactcc agcgggatct cgaccgtcag gtcggtcccg cccccgtcgt tgtcgccaag
    10801 ccggagagcg ccacccagcg caacgactct ttcctgaatc tccaccagac caagcccccg
    10861 tgcgcccgaa cgacgggcca cggccgcggc gtcgaacccg acgccgtcat ccagaatcga
    10921 acacgcgacc ttgtgcgact cgcggcggag acagaccgat gcctgagtcg ctttcgcatg
    10981 cttgacgacg ttgttgagcg cctcctgggc gatgcggtac agcgtggtct ccacagtcgc
    11041 cggcaggctg cgatcgaggg tcgcatgcac ggccacgccg actccccacc gcttcgatat
    11101 ggtgtccgcg aggaactcga gcgcgggcac cagcccgaga tcgtcgagga ttggcggccg
    11161 gagctcgtgc gagagattcc gcagccgatc ctcgatctgc tcgaggagcc cacgaacgcc
    11221 ctgcaccggt ttcgccgtac cgggctgcct gaccgccagg tccgcgagcg ccaggtgcac
    11281 cgatgccagc aactgcgcgg cctcgtcatg gagggccgac ccgatgcgcc tggcctgccc
    11341 ctccaggacg tcgttcacgc gatgcagcac gacgttggac tcccgaaagc ccagaaacgc
    11401 catctcgaac ggcgacagcg cttcgacgag aaacttctcg agcgcctcca gcagcttggc
    11461 gtgttcgtgc tcgtggagcg gtcgcaggag caaggcgggc agcgcgcgtg agtggacggt
    11521 ggcaacgtcg agcacaccca ggccgtcctc gagcgccttg cgtcccagct cgtacgcccg
    11581 cgccagggcg gcctcgtcct ggttggacac gtattggcgg agcgccatcg aatagtcgtc
    11641 cgacagcgtg cacaggcgat cgctcatggc gcctcgcctc cgaggtatcg cgcaaccatc
    11701 acgagggcat cgtccactcc gctgcgatgt cgggccatga tgccggcggc aatgccctcc
    11761 gggtcgccgt tgagggcaat cccgtccgcg aagtcgggcc tgatgccgtc ggtcgccaca
    11821 atcagcgtgt ccagtggttt cagcgtcacg acctctgcgc gcaacctcgg tgggaggcgg
    11881 taccccacga cacctccgcg gagcagcgcg cgctcgggcg actcgcgctc cgtgccgtct
    11941 tcgtgaacga gtacgccctc gacgttgccg acgccaagcc atgtcagggt gcggtcgcac
    12001 gagtcgagaa gggcgaccgt catggtgacg ccccggctgc gtttgagagc gtcatgacac
    12061 cgaagcacca gcgagaccgg ggagtcgctg acgtgcgccc tgagcaccgc cgccgcggct
    12121 ttcgcggccg cggccgcgtc ggatccatga ccgacgccgt cgatgacagc gatcagcacg
    12181 ccgtcgcttc gggtctccac gacgaacaga tcacccgact ccggctcggc cggcagcggc
    12241 atttcggcga cgccccaggc gacgacgcct ccagggcaca ccgtgcggcc gtggtgttgc
    12301 ggcatcgtca cactgcccat ttcttcatcg tcacggtcgt gcccttgccg acctcggacg
    12361 cgatctcgaa ttcgtccatc aaccgtctcg agccgggaag accgatgccc aggcccttgc
    12421 ctgtggagta gccgtcgcgc atgaccgtgg caatgtcggc gataccgggc ccgtgatcga
    12481 gcgccacgat cttgatgccc gccgaacccg cgttgttgag gagcgtcagc acgacctctc
    12541 cctccttcgc gtattcgacg atgttcctcg cgacctcgga gatggcggtg gcgatgatcg
    12601 tcaggttgct gttcgagaag cccgcctgcg ccgcgagcgc acgaccctgc tggcgggcaa
    12661 tcacgatgtc ggtggatgac tggatccgga cccggacttc aggctccatg accgtcctcg
    12721 agccgcagtt tcaagaacag cagcccctcc tcgagatcca gggccgtggg tacgttctcg
    12781 agcgtcaacc cgagctgcac catcgacatg gcgacctcgg gttgtatgcc cacgatgacc
    12841 gtctccgcgc cgcgaagctt ggtcgtgtag gcgatggcgc gcagcgtgcg ggtggcgaac
    12901 gagtccagga catcgagcgc ggtgacgtcg acgatcacgc cccgcgcgcg gtgccggcca
    12961 accaggtcgg ccaaatcatc gcgcagctga accaagtcct ggtcgctcag cgcggactga
    13021 atgctggcga tcaggaagtg actctgcttg agaatcggaa cggccatcgt gcccccgctg
    13081 ttcggacgcc catggtgaag cgacgtgccg gtgctcgcgc gcgaccaccg gcatccctac
    13141 catcgtgaag cgccgccgcc tctcccccgg gtgatgtgaa ctactggacg cgcgggggaa
    13201 tttcctcgag ggcaaccgcc ttgaagccca acaggcgctc ggcgagctcg atcccgcctt
    13261 gcaggtcgcc gacggtgttc atcttactca ggtcgacgcc gatggtaacc agtgtctggg
    13321 cgatttcggg ggagaggccc gtgacaatga ccgtggcgcc gagcagccgc gcggcttcga
    13381 cggtttgcac caatcggttc gcaatggctg agtcgatcac gggaacaccc gtgatgtcca
    13441 tcacggcgac tttgccgcgg ttctcgcgta tgccgcgcag gagttgctcg gtgagctgcc
    13501 gcgcgcgctg cgggtcgatc aatccgatga tcggcagaat cagcagccgc tcacgcacgc
    13561 ggaggaccgg agtggagagc tcacggatgg cctcctgctg ctgccggatg atccgttccc
    13621 gctcctggac gaagccgacg gccaccgtgt tcgcaatccg gttcgcggcg ggctcatacg
    13681 catcgagcac gaagttgagc agcttgaaat cgagctggta cttcttgaac agcgagcggg
    13741 ccagcacgtc gcgcagcagc agcacgatgc ccagcacttc ctgcgtttcg acgcctctcg
    13801 ggatgatgcg ttccgacagg tcgcgcgcgt aggcctggag cgcttcgacg cttcccgtct
    13861 cgagcactgc gacgtagttg tcgtacaccg tggtggcttc ggcgaagatc tcctcacgcg
    13921 tcatcgcggt aagcagccgc gactccgtga tgcgccgcgc ccattcctcg cggagctcgg
    13981 tccggttctc acgcaggtgg gacaccagct cgcgcaagag agccgtcgtg gattcgcgat
    14041 tcgaactggt catcggctga accacatggt tcttctcacg cgccatacac tcccccttcc
    14101 gggcaccttt cgtgacgtgt ccccggggtc gcagcccgga gacccatgag tatgcgggag
    14161 ccgaaagaac ccgggtaatc aggtattgtc cctactccgc tcgggaaaac acccgacgtc
    14221 cgtgggcaca cctgcctcct cctccttgcg ccgggtccgg cgggcggcat agtctacagc
    14281 ctccattacc ggccctccag caggagaatc acgatgatcc gcagacatct cggcggcatt
    14341 ctggtcgccg tggccctcgg gatcgctgct gccgcagccc agacgcagac gcagaagccg
    14401 aacccgcaga accaggcaac cgaggacttc gaggctcctg ccgcgtttcc tctggccgcc
    14461 ccgaagggca ccgacagccg cgcgatgacc gaggcgccgc ccaaggcagt caataccggt
    14521 cccttcgatc ccgcgacctg gaaatacggc accgcgttcg cgccgccgcc ggggacgaag
    14581 atctggaatc cggtgaagct gaagatgctg cagggcggca aggtcaccgg cggaacgctc
    14641 ttcagcgcga cggatcccgc cacctactgt gcgatggccg acgccggcta cgacttcatc
    14701 tggaccgaga tgcagcacga ccagcgcgac tgggaccagg cggcgcagat gtggcggacg
    14761 tgcccgtacg cgcgcgcggt tccaggggtg cgcgtggcaa gggccgagga gcgcgagatc
    14821 cagcacgcgc tcgacgcggg cgcgctcgtc gtcgccgtgc cgacggtcga tacggtggaa
    14881 gaggcgatcg aggcccgcaa ctggacgtac tttccgccgc tcggccgacg aagcgcgggc
    14941 ggcggacagg cgttcgaaca ggacatgtgg ggtcgcgtgc cggggggtta tcgaaacacc
    15001 gccaacgaca acatcgtcct cattctcatg atcgagacgc tggagggcct caagaacgcg
    15061 gaggccatcg cgaaggttcc cggggtgacg gccttgttcg ccgcgagcgg ggacctgggg
    15121 aatttcaccg ggttcaggca gggttcaccc gattacgaac gcgtgatcaa catcgtgcac
    15181 gacgccgcca tcaaggcggg ggtgcgtctg tgcggtccgc tcgcgtggcg ggatcgtcc
//
LOCUS       DBNGSH010000004         5356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4075493, whole genome shotgun sequence.
ACCESSION   DBNGSH010000004 DBNGSH010000000
VERSION     DBNGSH010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4075493"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="AC16CY_00110"
     CDS             <1..531
                     /locus_tag="AC16CY_00110"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF015113.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase anticodon binding
                     domain-containing protein"
                     /protein_id="HIR3352204.1"
                     /translation="VLPRAQAIRDELVGRGVRVLIDDRETQTPGWKFNEWELRGVPLR
                     LEIGPKDIEKSQVVLARRDTREKASAPMEGLSAHVVQLLETIQRALFDRAIAFRDDHT
                     SRADSYAELKQIMEGRPGFVVSPWCGSAACEAEVKTETQATIRNIPFNGTSAAGKTCV
                     KCGRAATVHAWFAKAY"
     gene            549..935
                     /locus_tag="AC16CY_00115"
     CDS             549..935
                     /locus_tag="AC16CY_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352205.1"
                     /translation="MRKLVVGTLILLCVAGCKPNTPLRVSTIQTGRSLNSDRSIGQHT
                     TRFKPDDTLHVAVLTDGPGAGAITVRWRYRSSLVSEETQEVAYRLNAATEFHITNSSG
                     FPEGDYTVEILVDNKPFATRTLSVVK"
     gene            complement(961..1863)
                     /locus_tag="AC16CY_00120"
     CDS             complement(961..1863)
                     /locus_tag="AC16CY_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352206.1"
                     /translation="MNGSSQIDRLIADLQSSDPVRRDAAVARLRVLGTRALPRLTKFL
                     ESGAPAPAHALAISTLEGVEDRRAMTIALNALESADADAVVAALAVLRGWVAKETGTR
                     VLEAVTAIAVDRSREGRIRFAASEALSDLPEHLVRPIKEQAPPPASVGPPLDDAVAAR
                     EWVEAHGRGATLATLHDAVKAFREREESAASGRRRDAWLLARSTTHRVLAERGSRLAL
                     YDLREAFSTAQSPLPPGFLAAVVKAGDASCLEPLARAWSASGRGSAWRNQLSEAGREI
                     VRRSKLGGRSPVIKGLRAKWPGFV"
     gene            complement(2113..2952)
                     /gene="tatC"
                     /locus_tag="AC16CY_00125"
     CDS             complement(2113..2952)
                     /gene="tatC"
                     /locus_tag="AC16CY_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3352207.1"
                     /translation="MALVPFPSKSADEDPEWEDEESESSQGKMSFLEHLDELRRRIIY
                     SVLSLVVGVIIACFFLGEGSWRGPILPEGLYNFVMVPMQEALGPDAKMVFTEPTEGMM
                     LYIRIALIAGLLIAIPAILTQVWLFVAPGLYANEKRLALPFVLMGSVFFIAGAAFAHE
                     IVFPLTWRFFGSFANEYVDFLPRIEPAFSLYMKLVLIFGAIFQMPTLVLFLAKMGVIT
                     ARFLVRNMKYAILIIFIVGAVLSPGGDPMGQVLMAAPMFILYCISIVLAWLFGKKRKP
                     AEA"
     gene            complement(2964..3383)
                     /gene="tatA"
                     /locus_tag="AC16CY_00130"
     CDS             complement(2964..3383)
                     /gene="tatA"
                     /locus_tag="AC16CY_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR3352208.1"
                     /translation="MYTLALTPSRLDFYNPENDLQTSGRPAPEPAGARIMFPNIGIPE
                     LIIILGIALIVFGPRKLPELGRSLGRSLGEFRKASNELKHTLEEEVRIEEQRDQRAKM
                     QAEQASAVAAATPAPIEPGSTGALPTEESTVSRTTAG"
     gene            3403..5034
                     /locus_tag="AC16CY_00135"
     CDS             3403..5034
                     /locus_tag="AC16CY_00135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3352209.1"
                     /translation="MRSYRYLSAAALVIALSAIAGGLWGHSALVAQDQIPDQYKVFTA
                     ALGAIEDNYVGEFESDRLVYSAISGMLQTLDPHSSFMDPRSFAQMRERQEGRYYGLGI
                     SINVVNGDVTVFNVFEGSPAHQMGLRRGDVIARIEGEDAKGWTSEQAVTRLRGPKQTA
                     VKISLRRVGYDGLIDLAVMRDEVHIPTVLAAVMLDSSTGYIKLQDFGENTDQELGRAL
                     DDLTKKGMKRLVFDLRGNPGGALDQAIRVSNRFLAKGELIVYTRGRVPNSDQDYRATE
                     RSDYLNVPMITLVSRTSASASEIVSGALQDHDRSLIVGETTFGKALVQSVYRVAEGAG
                     AAITTARYYTPSGRLIQRPWDGSFDEYLTYIYREQDATKAHKPEDLKYTDAGRKVYSG
                     GGIEPDRRFDGPSEGFNPSRFARSIAARNLFDTYAQQFARRGDTRVGRRSSQGTKELD
                     ADFEVTDEMLAEFKTLVQASPIPFDEAAWQQDIEFLKAMIHREIDVDLFGLAAAYKNL
                     ARRDPQLQFALGLFPEAQQLLNLSRQPAAGRRASR"
BASE COUNT          984 a         1715 c         1749 g          908 t
ORIGIN      
        1 gtcctgccgc gcgcccaggc gattcgcgac gagctggtcg gacgcggcgt ccgcgtcctg
       61 atcgacgacc gcgagaccca gacgcccggc tggaagttca acgagtggga attgcgcggc
      121 gttccgctgc ggctcgagat cggtccgaag gacatcgaga agtcgcaggt cgtgctggcg
      181 cgccgagaca cgcgcgagaa ggcctccgct ccgatggaag gcctctccgc ccacgtcgtg
      241 caattgctcg agaccatcca acgggcgctc ttcgatcgtg caatcgcgtt ccgggacgac
      301 cacacgtcga gggctgattc ctacgccgag ctcaagcaga tcatggaagg acggccgggg
      361 ttcgtcgtgt cgccctggtg cgggtcggcg gcgtgtgaag cggaggtcaa gacggaaaca
      421 caggccacga tccgcaacat tccgttcaac ggtacgtccg cggccgggaa gacgtgcgtg
      481 aagtgcggcc gggccgcgac ggtgcatgca tggtttgcaa aggcgtatta aggtgcgagg
      541 atgcgagggt gcgtaagctc gtagttggga cgttgattct tctgtgcgtg gccgggtgca
      601 agccgaacac cccgcttcgc gtctcgacga ttcagaccgg gcgctcgctc aattccgacc
      661 gcagcatcgg ccagcacacg acgcggttca agccggacga cacgttgcac gtggcggtgc
      721 tgaccgacgg ccccggcgcg ggggccatca ccgtgcgctg gcgctaccgc tccagcctgg
      781 tgagcgaaga gacgcaggaa gtcgcgtatc gcctcaacgc ggccactgag ttccacatca
      841 ccaactcgag tggattcccc gagggcgact acaccgtaga gatcctcgtt gacaacaagc
      901 cctttgcgac gcgcacgctg agcgtcgtga aatagacagg cgagcatcca gccccagggc
      961 ctagacaaat ccgggccact tggcgcgaag tcccttgatc accgggctcc gtccgccgag
     1021 cttcgaccgc cgaacgattt cacgtccggc ctctgagagc tgattccgcc aggcactgcc
     1081 ccttcccgac gccgaccacg cccgggcgag cggttcgaga caactcgcgt cgccggcctt
     1141 cacgaccgcg gcgagaaacc ccggcggaag aggcgactgt gccgtgctga acgcctcgcg
     1201 aaggtcatag agcgcgaggc ggctgccgcg ttcagcgagc accctgtgcg tggtcgagcg
     1261 cgccagaagc catgcgtcgc gccgccggcc ggaggccgcg ctttcctcgc gctcccggaa
     1321 tgctttgacg gcgtcgtgca gcgtggccag cgtggccccg cgtccgtgcg cctcgaccca
     1381 ctcgcgcgca gcgaccgcat cgtcgagcgg cggaccaacg ctggccggtg gcggcgcctg
     1441 ctccttgatc gggcggacca ggtgctcggg caagtcggag agcgcctccg acgcggcgaa
     1501 tcggatccgg ccctcgcggc tgcgatcgac ggcgatggcc gtcaccgcct cgagcacgcg
     1561 tgtacccgtt tccttcgcca cccacccgcg caggaccgcg agagcggcaa ccacggcatc
     1621 ggcgtccgcc gattcgaggg cgttcagcgc gatcgtcatc gcgcgccgat cttccactcc
     1681 ttcgagcgtg gagatggcga gcgcatgggc gggggccggg gcgccggatt cgaggaactt
     1741 cgtcaggcgg ggaagcgccc gcgttccgag aacgcgcagg cgggcgactg cggcatcgcg
     1801 ccggaccggg tcgctggatt gaaggtcggc gatcagccgg tcgatctgag aactaccgtt
     1861 cacacttctc ccttctccct tctcggttcg tgttcaaatt cgggggttcg tgttcgaggt
     1921 tctgggttcg atgttccccg atggcggttc gtggttcgag cgaaatcgct gtggggcgtt
     1981 tgcggcaaat cgcccgcgaa cgccgaaccc caaaccgaga acacgaacct gaaactgaac
     2041 acgaaccgag aaggctagaa ccgagaagtg tgaacgacag gtctcatggt cagttcacgt
     2101 ccgccaacgg gatcacgctt ctgcgggctt cctcttcttg ccgaagagcc aggccaggac
     2161 gatgctgatg cagtagagga tgaacatcgg cgcggccatc agcacctggc ccatcgggtc
     2221 gccccccggc gacagcaccg cgccgacgat gaagatgatc aggatggcgt acttcatgtt
     2281 ccgcacgagg aagcgcgccg tgatgacgcc catcttcgcg aggaacagta cgagcgtcgg
     2341 catctgaaag attgcgccga agatcagcac gagcttcatg tacaggctga acgcgggctc
     2401 gatccgcggc aggaagtcga cgtactcgtt ggcgaagctg ccgaagaacc tccacgtcag
     2461 cggaaagaca atctcgtgcg cgaaggcggc gcccgcgatg aagaagaccg atcccatcaa
     2521 cacgaacgga agcgcgaggc gcttctcgtt ggcgtagagt ccgggcgcca cgaagagcca
     2581 gacctgcgtc aagatggccg gaatcgcgat caggaggccc gcaatcaggg cgatcctgat
     2641 gtagagcatc atgccttcgg tcggttcggt gaacaccatc ttcgcatccg gcccgagcgc
     2701 ttcctgcatc gggaccatca cgaagttgta cagaccctcc ggcagaatcg gtccccgcca
     2761 ggacccttcg ccaagaaaga agcaggcgat gatgacgcca acgacgagcg agaggacgga
     2821 gtagatgatc cggcggcgca gttcgtccag gtgctcgagg aaggacatct tcccctggga
     2881 ggattcggat tcctcgtcct cccactcggg gtcttcgtcc gccgacttgc ttggaaaagg
     2941 cactagggcc atgacggact gcgctacccc gcggtcgtgc ggctgactgt ggactcctcg
     3001 gttggcagcg cgccggtgga tccgggttcg atcggcgcag gggttgccgc ggccaccgcc
     3061 gacgcctgct ctgcctgcat cttcgcgcgc tgatcgcgct gctcctcgat ccggacttct
     3121 tcttccagcg tgtgcttgag ttcgttcgac gccttgcgga actccccgag gctcctgccg
     3181 agcgaacgac cgagctcagg cagcttgcgc ggcccgaaga cgatcaacgc gatacccagg
     3241 atgatgatca gttcgggtat tccgatgttg gggaacataa tgcgtgctcc agccggctcc
     3301 ggcgccggcc ttcccgacgt ctgcaagtcg ttctctggat tatagaaatc cagtctagac
     3361 ggggtcaagg caagggtgta cattgctagt aatatgcttg gaatgcgcag ttaccggtac
     3421 ctgtcggccg ccgccctcgt cattgcgctc agcgccatcg ctggcggcct ctggggccat
     3481 agtgcgctcg tcgcgcagga ccagattccg gaccagtaca aggtctttac ggccgctctc
     3541 ggcgcgattg aagacaacta cgtcggcgag ttcgagtcgg accgcctggt gtacagcgcg
     3601 atcagcggca tgctgcagac gctcgacccc cactcgagct tcatggatcc gcgcagcttt
     3661 gcacagatgc gggagcgcca ggaagggcgc tactacgggc tgggcatctc gatcaacgtg
     3721 gtcaacggcg acgtcaccgt gttcaacgtc ttcgagggct cgccggcgca ccagatgggc
     3781 ctgcgccgcg gcgacgtgat cgcgcgcatc gaaggggagg acgccaaggg gtggacgagc
     3841 gagcaggcgg tgacccggct gaggggccca aaacagaccg ccgtcaagat ttcgctccgt
     3901 cgcgtgggat acgacggatt gatcgacctt gccgtgatgc gggacgaggt ccacatcccg
     3961 acggtcctgg cggccgtcat gctcgatagc agcaccggtt acatcaagct ccaggatttc
     4021 ggcgagaaca ccgaccagga gctcggacgc gccctggatg atctgacgaa gaagggcatg
     4081 aagcgcctcg tcttcgacct caggggcaat ccgggcggcg cgctcgatca ggcgatcagg
     4141 gtgtcgaacc gcttcctggc caagggtgag ctcatcgtgt atacgcgcgg gcgcgtgccg
     4201 aattccgatc aggactatcg cgccacggag cgaagcgatt acctgaacgt gccgatgatc
     4261 acgctcgtca gccgcacgag cgcgagcgcc tcggagatcg tgtccggcgc gctccaggat
     4321 cacgatcgtt cgctcatcgt cggcgaaacg accttcggca aagcgctcgt gcagtcggtc
     4381 tatcgcgtgg ccgaaggcgc cggcgcggcc atcacgacgg cgcgctacta cacgccgagc
     4441 ggccggctga tccagcgccc gtgggacggc agcttcgacg agtacctgac ctacatctat
     4501 cgcgaacagg acgcgaccaa ggcgcacaag cccgaggatc tcaagtacac cgacgccggg
     4561 cgcaaggttt acagcggggg gggcatcgaa cccgatcgcc gtttcgatgg tccttccgaa
     4621 ggcttcaatc cgtcccggtt cgcgcggtca atcgccgcgc ggaacctgtt cgacacctac
     4681 gcgcagcagt tcgcgcggag gggtgatacg cgcgtgggac gccgctcgtc ccagggcacg
     4741 aaagaactcg acgcggactt cgaggtgacc gacgagatgc tcgccgagtt caagacgctc
     4801 gtgcaggcct cgcccatccc gttcgacgag gctgcgtggc agcaggacat cgagttcctc
     4861 aaggcgatga tccatcgcga gatcgacgtg gatctgttcg gcctggcggc ggcgtacaag
     4921 aacctcgctc gccgggatcc gcagctgcaa ttcgccctcg gtctgtttcc ggaagcgcag
     4981 caactgttga acctgagccg ccagccggcg gccgggcgac gggcctccag gtgagttaag
     5041 acaactccca actcccaact cccaaggcgc tcggttcctg gggaattggg agttggacgt
     5101 tgggagtttt ggccttggcc caaaatatag tggtgtgact ttggtcttgc cacaatagct
     5161 gggccactgt tagactcccc gcgtcggtcg gtccgaccaa gctgcccgcc gccccgtcct
     5221 cccagtacgc cggccgggtc cggtgcgcgt gtcgggcagg aagggtaatg cgtctcgaac
     5281 gcctcgaaat ctccgggttc aagtcgtttt ccgatcggtc ggagctttcc ttcgaccagg
     5341 gcgtcaccgc catcgt
//
LOCUS       DBNGSH010000005         3080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3396317, whole genome shotgun sequence.
ACCESSION   DBNGSH010000005 DBNGSH010000000
VERSION     DBNGSH010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3396317"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /gene="larB"
                     /locus_tag="AC16CY_00140"
     CDS             complement(<1..755)
                     /gene="larB"
                     /locus_tag="AC16CY_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR3352210.1"
                     /translation="MTESELQKLVDDVAAGAIAADEASARILDALRSAPYDDLGFARV
                     DQHRHLRQGFPEVVLGIGKTPAQIAAIAGRIVAHGHSLLVTRATLDAYDAVRETVPTA
                     VFHGEARAITLAQGEIARGHGTVLIACAGTSDLPVAEEAAVTAALMGNAVDRLYDVGI
                     AGLHRLLHERERLERARVLIVVAGMEGALPSVIGGLVRVPVIAVPTSIGYGASFGGIA
                     ALLGMLNSCASGVAVVNIDNGFGAACLASRINHL"
     gene            856..2349
                     /locus_tag="AC16CY_00145"
     CDS             856..2349
                     /locus_tag="AC16CY_00145"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HIR3352211.1"
                     /translation="MTASESLVLGTYFFVLVILAVYGWHRYYLVYLYMKNKSRQPVPA
                     GPMDALPAVTIQLPIYNEMYVADRLIDAVCRLEYPRELLEIQVLDDSTDETRGVAEQA
                     VRRHAAAGIDITYLHRTDRTGYKAGALDAGMKVAKGEFIAIFDADFIPTPDFLTRTVP
                     FFVDPAIAMVQARWGHINQDYSLLTKIQSILLDGHFVLEHGGRNRSGLFFNFNGTAGI
                     WRRAAIVDGGGWQHDTLTEDLDLSYRTQLRGWKFVFLPELIAPAEVPVEMNSFKSQQH
                     RWAKGSVQTCRKLLPRILQSPLPLGVKAEAFFHLTANFNYPLMCVLSVLMAPAMVIRY
                     NMGWYEMLLIDVPLFFAATASVANFYLVCQRELHDDWIARIKYLPFLMSIGIGLTVNN
                     TRAVFEALFDRQSEFARTPKYRIEAAADEWITKKYRQSFVVQPMIELALGLYFTATVF
                     YALANGIYGTLPFLVLFQVGFLYTGLLSIIQQYAGGLVFEPVAGEEL"
     gene            2491..>3080
                     /locus_tag="AC16CY_00150"
     CDS             2491..>3080
                     /locus_tag="AC16CY_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352212.1"
                     /translation="MSNQTERPDLEVSATDLLPLHDESVRLEQRETTGRWWLAVVGLA
                     TVLGGLTMILVLGWLNAQPAAEAAGAAHAAPSTPKVIVESAPPPKWAGRRQATWARDG
                     SKTIAFELQAANEVALWMQRSRPLLVVRCVSRATEVFVAIGSAASFEAQAGSHTVRIK
                     IDDDPELVQQWSDSESGQELFAPDGIALARRLADAE"
BASE COUNT          519 a         1048 c          981 g          532 t
ORIGIN      
        1 aggtgattga tcctgctggc gagacacgcg gcgccgaagc cgttgtcgat gttcacgacg
       61 gccacgccgc tggcgcacga gttgagcatg ccgagcagcg ccgcgatgcc gccgaagctg
      121 gcgccatagc cgatgctggt cggcacagcg atcaccggca cgcgaaccag gccgccgatc
      181 acgctgggca gcgcgccctc catgccggcg acgacgatga gcacccgcgc gcgctcgagg
      241 cgctcccgct cgtggagcag tcgatgcagg ccggcgatgc cgacgtcgta gagccgatcc
      301 acggcgttgc ccatcagcgc cgcggtgacc gccgcttcct cggccacagg caggtccgag
      361 gtgccggcgc aggcgatgag caccgtgcca tggcccctcg cgatctcgcc ctgcgcgagc
      421 gtgatcgccc gtgcttcgcc atggaagacc gcagtcggca ccgtctcgcg gaccgcgtcg
      481 taggcatcga gcgtcgcgcg ggtcaccagg agcgagtgtc cgtgagcgac gattcggccc
      541 gcgatcgcgg cgatctgcgc gggcgtcttg ccgatcccga gcacgacctc gggaaacccc
      601 tggcgaagat gcctgtgctg gtccacgcgg gcaaagccga ggtcgtcgta cggggccgag
      661 cggagcgcgt cgaggatgcg ggccgaggcc tcgtcggccg cgatcgctcc ggcagccacg
      721 tcgtccacca gcttctgcag ttcggattca gtcatcgctc gagcgcgcaa gacgcccaca
      781 aaaggcccgt ttcattgact ttacgcaaca cgacaatata atcgccttcg tgcccccctg
      841 gcacattcgc gtcgcatgac ggcctctgaa tcgctcgttc tcggcacata ctttttcgtt
      901 ctcgtgatcc tggcggtgta cggatggcac cggtactacc tggtctatct gtacatgaag
      961 aacaagagcc ggcagccggt tcccgccggt ccgatggacg cgcttccggc ggtcacgatt
     1021 cagcttccga tctacaacga gatgtacgtc gccgatcggt tgatcgacgc cgtctgccgt
     1081 ctggagtacc cgagggagct gctcgagatc caggtcctgg atgactcgac cgacgagacg
     1141 cgcggcgtgg ccgagcaggc cgtgcgccgg cacgccgccg ccggcatcga catcacctat
     1201 ctccatcgga ccgatcgaac cggttacaag gccggggccc tcgatgccgg catgaaggtg
     1261 gccaagggcg agttcatcgc gatcttcgac gccgacttca ttcccacgcc cgacttcctg
     1321 acccgcaccg tgccgttctt cgtcgatccg gccattgcca tggtccaggc gcggtggggg
     1381 cacatcaacc aggactactc gctgctgacg aagatccagt cgatcctcct cgacggacac
     1441 ttcgtcctcg agcacggcgg ccgcaatcgg tccgggctct tcttcaactt caacgggacg
     1501 gccggcatct ggcgccgcgc ggccatcgtt gacggaggcg gctggcagca cgacacgcta
     1561 accgaggatc tcgacctgag ctaccgcacg cagcttcgag ggtggaagtt cgtgttcctg
     1621 cccgagctca tcgcgccggc cgaagtgccg gtggagatga actccttcaa gtcgcagcag
     1681 catcgctggg cgaagggctc cgtgcagacc tgccggaagc tgctgccccg gatcctccag
     1741 tcgccgctgc cgctcggcgt gaaggcggag gccttcttcc acctgacggc caacttcaac
     1801 tatccgctca tgtgcgtgct gtcggtgctg atggctccgg ccatggtcat ccgctacaac
     1861 atgggctggt acgagatgct gctgatcgac gtgccgctgt tcttcgcggc aacggcgtcg
     1921 gtcgccaact tctatctcgt gtgccagcgc gagctgcacg acgactggat cgcccgcatc
     1981 aagtacctgc cgttcctgat gtcgatcggg attggcctga ccgtgaacaa cacgcgcgcg
     2041 gtgttcgagg cgctcttcga tcgccagagc gaattcgccc ggacgccgaa gtaccgcatc
     2101 gaggcggcgg ccgacgagtg gatcaccaag aagtaccgac agagcttcgt cgtgcagccg
     2161 atgatcgagc tggcgctcgg gttgtacttc accgccaccg tcttctacgc actcgccaac
     2221 ggcatctacg gaacgctgcc gtttctcgtg ctcttccagg ttggcttcct ctacaccggc
     2281 ctcctgtcga tcattcagca gtacgccggc ggcctggtgt tcgagccggt ggcgggcgaa
     2341 gaactgtaaa ccactcgaaa ctcaagactc aaaactcaaa actgctctcc cgtccacccc
     2401 ttttgtaagc gcgatcgcca cgacaccccg atgtccggcg ggcgccacgc tggcatacga
     2461 attgcgggac gctggttgga cgatcgagct gtgtctaacc aaaccgaacg ccccgatctg
     2521 gaagtgtccg cgacggactt gctgcccctg cacgacgaat ccgtgcgcct cgagcagcgt
     2581 gagacgaccg gccgctggtg gctggccgtg gtcggcctgg ccaccgtgct cggaggcctc
     2641 acgatgatcc tcgtgctcgg ttggctgaac gcgcagcccg cggccgaggc cgccggtgca
     2701 gcgcacgccg ctccgagcac gccaaaagtg atcgtcgagt ccgcgcctcc gccgaaatgg
     2761 gccggccgcc ggcaggcgac ctgggcgcgc gacggttcga agacgatcgc cttcgagctg
     2821 caggcggcca atgaagtggc gctctggatg cagcgctctc ggccgcttct tgtcgtccga
     2881 tgcgtgtccc gcgcgaccga ggtgttcgtg gcgatcggct cggcggccag cttcgaagcg
     2941 caggcgggca gccataccgt gcgcatcaag atcgacgacg accccgaact ggtgcagcag
     3001 tggtcggatt ccgagagcgg ccaggagctc ttcgcgcccg acggcatcgc gctcgcgcgg
     3061 cgactggcag atgccgagca
//
LOCUS       DBNGSH010000006         3124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2547328, whole genome shotgun sequence.
ACCESSION   DBNGSH010000006 DBNGSH010000000
VERSION     DBNGSH010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2547328"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="AC16CY_00155"
     CDS             <1..626
                     /locus_tag="AC16CY_00155"
                     /inference="COORDINATES: protein motif:HMM:NF052887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF8021 domain-containing protein"
                     /protein_id="HIR3352213.1"
                     /translation="EHVFARIAAPAQIAALQTPRPAFLATVPQAERMPRSWMFLLANA
                     YYDSLVLGDGELTPFADDCGRRENGMHTAGVGRPAPTGPPRAGGPGGPPGGGPQLPTG
                     CAEQLTARAMTYIGSIDLRRIWIADEEKGLVFAYTMFRHPLDAPNKNYPVLLPDGTIS
                     DRTMNFEPFDLEAAHIYKIFGGKMHEIEAMGFTLPLNSKNGWSPFIK"
     gene            complement(727..1488)
                     /locus_tag="AC16CY_00160"
     CDS             complement(727..1488)
                     /locus_tag="AC16CY_00160"
                     /inference="COORDINATES: protein motif:HMM:NF042851.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HIR3352214.1"
                     /translation="METYRAHRVLRADRSGSSWPVTVDTDAGVFYTKLRGAAQGPTSL
                     VAEIVVGALADALGLSVPARVLIDIPPGIPIDDPHQELGQLLRSSIGRNLGFQLLPNA
                     QSFRPGDERRVDHELASTIVWLDGLVQNPDRTVKNPNLLWSHNQLWLVDHGAALGFQH
                     DWPAVTEASPRSGRWSVTDHVLRSRATRVAAMDESLATRVHRGALESALDGVPDDFLV
                     EDGGEALRRRRAAYVAFLWKRLRSPRPFVSGGPAG"
     gene            complement(1521..2510)
                     /locus_tag="AC16CY_00165"
     CDS             complement(1521..2510)
                     /locus_tag="AC16CY_00165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3352215.1"
                     /translation="MRVLVTGSAGHLGEGLVRTLREHRHEVVGLDLVESPFTTHVGSV
                     SDRDLVRSCLHGVDVVIHAATLHKPHVATHSRQAFVETNVAGTLTVLEAALESGVSGI
                     VFSSTTSAFGSALRPSDGQPAAWIDEDVESVPRNIYGVTKKAAEDLCELFFRSRGLPC
                     IVLRTSRFFPEADDDKELRRQYDDLNLKVNEYLYRRLDLADAVNAHILAAERTAEIGF
                     ARYVVSATSPFERAHLSQLRTDAPGVVRSLFPDYEEVYRPRGWRMLPGIDRVYVNHRA
                     QKELGWRPRFDFRGILDGLGRGDEVFSPLARAVGSKGYHAQVFEDGPYPVESG"
     gene            complement(2609..>3124)
                     /locus_tag="AC16CY_00170"
     CDS             complement(2609..>3124)
                     /locus_tag="AC16CY_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015110606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3352216.1"
                     /translation="HWDAAHIRERIVATYDQIIDQTRRNSDEFVWSSIRSVDELGQVR
                     MAAMEAFLSDYGQGRADGRYVEAELPTLPFADRSFDLALCSHLLFLYSAQLGEDFHRD
                     SLRELCRVAREVRVFPLLALGGQRSPYVDRSVADLRASGHDVTIESVPYEFQRGGNHM
                     LRIRTAKMTDG"
BASE COUNT          616 a         1042 c          938 g          528 t
ORIGIN      
        1 ccgagcacgt gttcgcccgc atcgcggcgc cggcgcaaat cgccgcgctg cagacgccgc
       61 ggccggcgtt cctggcgacg gtgccgcagg ccgagcggat gccgcgaagc tggatgttcc
      121 tcctcgccaa cgcctactac gactcgctcg tcctggggga cggcgagctc acgccgtttg
      181 cggacgactg cggccgtcgc gagaacggca tgcatacagc cggcgtgggc cggccggctc
      241 cgaccggccc accgcgcgcg ggcggacctg gcggccctcc cggcggcgga ccgcagctgc
      301 cgaccggatg cgccgagcag ttgaccgcgc gggcgatgac ctacatcggc agcatcgatc
      361 ttcgccgtat ctggatcgcc gacgaagaga aggggctcgt attcgcctac acgatgttcc
      421 ggcatccact cgatgcgccg aacaagaact atccggtgct gctcccggac gggacgatca
      481 gcgatcgcac gatgaacttc gaaccgttcg acctcgaggc ggcgcacatc tacaagatct
      541 tcggcggcaa gatgcacgag atcgaggcca tgggcttcac gctgccgctc aactcgaaaa
      601 acggctggag cccgttcatc aagtagtcgc ctcgcctcca tgtgggccat aagacaacac
      661 ccacacgttg aatcctgacg cgagcgccat ctcacgatcg aatcagacgt cggctgcaag
      721 caattgtcag ccagcgggac caccagacac aaagggccga gggctcctaa gccgcttcca
      781 gaggaacgcg acatatgccg ctcgacgacg acgcaatgcc tccccgccat cttcgactag
      841 aaagtcatcc gggacgccat ccaacgccga ctcgagggct cctcgatgga cacgcgtcgc
      901 cagcgactcg tccatcgccg ccacgcgagt ggctcgcgat cgcagcacat ggtccgtgac
      961 cgaccaccgg ccgcttcggg gcgacgcctc ggtcaccgcc ggccaatcat gctggaatcc
     1021 caaggcggca ccgtgatcga ccagccacag ttggttgtgc gaccacaaga gattcggatt
     1081 cttgaccgta cgatccgggt tctgaaccaa gccgtcgagc cacacaatgg tcgacgcgag
     1141 ctcgtgatcc acccgccgct cgtcacccgg acggaagctc tgagcgtttg gcaacagctg
     1201 aaatccgaga tttcgcccga tgctcgatcg aagcagctgc ccgagttcct ggtgcggatc
     1261 atcgatcgga atgcccggcg gaatgtcgat cagcacgcga gcggggacag acaatcccag
     1321 cgcatcggcc aaggcgccca ccacgatttc ggccacgagg ctcgtcggcc cttgagcggc
     1381 gccacggagc ttcgtataga agactccggc gtcggtgtcg accgtcaccg gccacgaact
     1441 gccactgcgg tcggcgcgaa gcacacggtg tgctcgatac gtctccatcg gagtgccgaa
     1501 gtgtcagact ggcagcaggt ttagccgctc tctacaggat aaggcccgtc ctcgaacacc
     1561 tgcgcgtgat atcccttgct gccgactgcc ctggcgaggg ggctaaacac ttcgtccccg
     1621 cgccccaagc cgtcgagaat gccccggaag tcgaagcgtg gtcgccagcc cagctccttc
     1681 tgagcacgat gattgacgta gacacgatcg atcccgggaa gcattcgcca gccgcgcggg
     1741 cggtacacct cttcgtagtc ggggaacaga ctgcgaacca cccctggagc gtcagtacga
     1801 agttgactca ggtgcgcccg ctcaaacggg ctggttgcac tcacgacgta tcgcgcgaat
     1861 ccgatttcag ccgttcgctc ggctgcgaga atatgcgcgt taaccgcatc agcgaggtcg
     1921 agccgccgat acaggtactc gttcaccttg agattgaggt cgtcgtactg tcgccgcagt
     1981 tctttgtcgt catccgcttc cggaaagaac cgggaggttc ggagaacgat acacggcaag
     2041 cctcgcgacc ggaagaacag ctcgcacaga tcctcagcgg ccttcttcgt caccccgtag
     2101 atgttcctcg gtaccgactc gacgtcttcg tcgatccagg ccgccggctg gccatctgag
     2161 ggtctcagtg cgctgccgaa tgcgctcgtc gtgctgctga agacgatgcc ggacacgccg
     2221 ctttccagtg ccgcctcgag aaccgtgagt gttcccgcaa cgttggtctc gacaaaggcc
     2281 tggcgtgagt gcgtcgccac atggggcttg tgaagcgtcg cagcatggat gacgacatcg
     2341 actccatgca ggcagctgcg aacgaggtct cgatctgaga ccgaaccgac atgcgtcgta
     2401 aagggcgact cgaccagatc gagacccacg acctcatgtc gatgctcccg cagggtcctc
     2461 acgagacctt cgccgaggtg cccagcgctt cccgtcacaa gcacgcgcat tcgatctcga
     2521 ttccttcgta gtctgcccca acggtccggt tcaccggcgc cgagaagcgg cgcgcgagcg
     2581 acgcggcggc gttaggtgca accggatgtt agccatccgt catctttgcc gtccgaatgc
     2641 gcagcatgtg gttgccgccc cgttgaaact cgtacggaac gctttcgatc gtcacatcgt
     2701 gacctgatgc gcgaaggtcc gcaacgctgc ggtcgacgta cggcgagcgc tgaccgccga
     2761 gagccagcaa cgggaagacc cggacctctc gcgcaacacg acacaactct cgaagcgagt
     2821 cccgatgaaa gtcctcgccg agctgagcac tgtagaggaa cagcaggtgc gagcagaggg
     2881 cgaggtcgaa ggacctatcg gcgaagggaa gcgtcggcag ttccgcctca acgtatcgac
     2941 cgtcagcccg tccctgcccg tagtcgctga ggaatgcctc cattgccgcc attcgaacct
     3001 gaccaagttc gtcgaccgag cgaatcgaac tccacacgaa ctcgtcactg ttgcgccgag
     3061 tctggtcgat tatctggtcg tacgtcgcaa cgatgcgctc ccgaatgtgc gcggcgtccc
     3121 agtg
//
LOCUS       DBNGSH010000007         1526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2037942, whole genome shotgun sequence.
ACCESSION   DBNGSH010000007 DBNGSH010000000
VERSION     DBNGSH010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2037942"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="AC16CY_00175"
     CDS             <1..284
                     /locus_tag="AC16CY_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR3352217.1"
                     /translation="FYAVEARLATRGMVRQAHRAGRDVYVWTVNDPAWMLSAMSHGVD
                     GLITDRPDVARQVIARRAGMSDAQRILVALLVRLGARTEVLAREDALRP"
     gene            337..>1526
                     /locus_tag="AC16CY_00180"
     CDS             337..>1526
                     /locus_tag="AC16CY_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006992493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virginiamycin B lyase family protein"
                     /protein_id="HIR3352218.1"
                     /translation="MIRSLSFGAALAFGSVALTAALHGQAPQGGGRGQVQLPDGAGKE
                     IVTASCASCHGLNMITGAAGYTQDGWRDLMSTMIEMPEAQAAAVARYLATHFPPRPGR
                     APVLVPGDVKVTFREWKVPTLGQRSRDPLQTRNGTIWWNGQFISLIGRLNPGTGEMVE
                     YELERTARPHSIVDDAAGNIWYMGNGNGTIGKLVPDTGVITVYKMPDPAARDPHTGIF
                     DKNGTLYFTLQQSNMVGRLVPSTGDIKLITLTTPRALPYGLKQNSQGTIWISYNGANK
                     LASMDPVTMEVREFPLPEGSRSRRLAITSDDMVWYVNSALGRLGRLNPKTGEIKEWPS
                     PSGPQSHPYAIEVVDDIIWYNESNQRPDALVRFDPKTEKFQSWAVPSGVGIIRHMRKT
                     PDGNL"
BASE COUNT          277 a          492 c          523 g          234 t
ORIGIN      
        1 acttctacgc ggtcgaagcc aggcttgcga cgcgcggcat ggtgcggcag gcgcatcgcg
       61 ccggacgcga cgtgtacgtc tggaccgtca acgatcccgc atggatgctg tcggcgatga
      121 gccacggtgt ggacgggctc attacggaca ggcccgatgt ggcccggcag gtcatcgcgc
      181 ggcgcgccgg catgagcgac gcgcagcgca tcctggtcgc gctgctcgtc cggctgggcg
      241 cgcgcaccga ggtgctcgcg cgcgaagacg cgctccggcc gtagccgtgg atttcgtatt
      301 acgcttgttc atagccggga tttcttggag gtcgccatga ttcgatcgct gagctttgga
      361 gctgcgttgg cgttcggttc agttgcgtta acggcggcgc tgcacgggca ggcgccccag
      421 ggcggcggcc gggggcaggt gcaactgcca gacggcgccg ggaaggagat cgtgaccgcc
      481 tcctgcgcaa gctgccacgg cctgaacatg atcaccggcg ccgccggcta cacgcaggac
      541 ggctggcgag atctgatgtc gacgatgatc gagatgccgg aggcgcaggc agctgccgtc
      601 gcgcggtacc tcgcgacgca cttccctccc aggcccggcc gcgcgcccgt tctcgtcccc
      661 ggcgacgtga aggtcacgtt ccgggaatgg aaggtcccga cgctcggcca gcgctcgcgc
      721 gatccgctgc agaccaggaa cggcacgatc tggtggaacg ggcagttcat cagcctgatc
      781 ggccgcctca atcccggcac cggcgagatg gtcgagtacg agctcgagcg caccgcccgg
      841 cctcacagca tcgttgacga cgcggcgggc aacatctggt acatggggaa cgggaacggc
      901 accatcggga aactggttcc cgacacggga gtgatcaccg tttacaagat gccggatccg
      961 gccgcgcgcg atccgcatac gggaatcttc gacaagaacg ggaccctgta tttcacgctc
     1021 cagcagagca acatggtcgg gcggctcgtg ccgtcaaccg gcgacatcaa gctgattacc
     1081 ctgaccacgc cccgcgcgct cccctacggg ctgaagcaga actcgcaggg caccatctgg
     1141 atctcgtaca acggcgcgaa caagctggcc agcatggacc cggtcacgat ggaggtccgc
     1201 gagttcccgc tgccggaagg atctcgatcc aggcggctgg cgatcaccag cgacgacatg
     1261 gtgtggtacg tgaactcggc gctcggccgc ctcggcaggc tgaacccgaa gacgggcgag
     1321 atcaaggaat ggccgtcgcc gagcggaccg cagtcgcatc cctacgcgat cgaggtcgtc
     1381 gacgacatca tctggtacaa cgagtcgaac cagcggccgg acgcgctggt gcggttcgat
     1441 ccgaagacgg aaaagttcca gagctgggcg gttccgtcag gcgtcggcat catccggcac
     1501 atgcggaaga cgccggacgg gaacct
//
LOCUS       DBNGSH010000008         2277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2038099, whole genome shotgun sequence.
ACCESSION   DBNGSH010000008 DBNGSH010000000
VERSION     DBNGSH010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2038099"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="AC16CY_00185"
     CDS             <1..905
                     /locus_tag="AC16CY_00185"
                     /inference="COORDINATES: protein motif:HMM:NF019869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3352219.1"
                     /translation="IYSRFFARVFRDIGLVDHSEPFTQLLTQGMVLKDGAVMSKSKGN
                     VVDPDTMLQKYGADALRLYVMFVAPPEKEVEWSDAGLEGSFRFLSRVWRLVDHWAGPL
                     ADRSTQRDDAPLDAAGKALRRKTHDTVRRVTMDIEQRQQLNTAVSALMELVNELYSFS
                     EETATGSPARHATAGAASATQERAEVVTVVAEALDALVRMLAPFAPHTGEELWERLGH
                     RGGLSAARWPAFDAAVAKAEQIVIPVQVNGKVRSRLTVPADTTEAELERLALADPPVM
                     AHTHGKTVRKVVVAKGRLVSVVVQ"
     gene            913..1458
                     /locus_tag="AC16CY_00190"
     CDS             913..1458
                     /locus_tag="AC16CY_00190"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HIR3352220.1"
                     /translation="MFTRFSGFTVIAILVSMSVGCGYALAGRGSFLPDYIKRVGIPTF
                     ENRSNVPDVGQVLTRKVRDEFIGRGKYEVTNETAGSDAVLTGEVLGITLQPIGLDSQT
                     QLASRYLFTLTVKVSFTDSKTSQVLWSNDALIFREEYELGARASSVTAGPAFFDQERS
                     LLDRLGGDAARSVVTSILEAF"
     gene            1464..>2277
                     /gene="holA"
                     /locus_tag="AC16CY_00195"
     CDS             1464..>2277
                     /gene="holA"
                     /locus_tag="AC16CY_00195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3352221.1"
                     /translation="MPRLSLSELRKQIASGQTRPLYALIGADDAEKSAIAAEFGDMVD
                     EGLRAFNVERMYGGDTTVKDLADAACTLPMLATRRVVIVLEAEKLLAPKRSSQAVEEE
                     QERLEAFLESPPDHASVVFVCGLLDMRRRVVKQLLKQAQIVDCGSIENEADAERWVRA
                     RAARDNVALDPAAARALVERTGPDLVRLRAGLERVALFAMGRAVTRDDVRLAVAPGPE
                     SQADFGIAKAIWRNDVRDALKELGLSLEAGSPPFMVLGQLRVAAEKQPAPQVK"
BASE COUNT          390 a          716 c          809 g          362 t
ORIGIN      
        1 tgatctactc gcggttcttc gcgcgcgtgt tccgggacat cggcctcgtg gatcactccg
       61 aaccgttcac ccaactgctc acgcagggca tggtgctgaa ggacggcgcg gtcatgtcga
      121 agtcgaaggg caacgtcgtc gatcccgaca cgatgctcca gaaatacggc gccgacgccc
      181 tcaggctgta cgtgatgttc gtggcgcctc ccgagaagga ggtcgagtgg agcgatgccg
      241 ggctcgaagg cagcttccgg ttcctgtcga gagtctggcg gctagtcgat cactgggccg
      301 ggccgctggc ggatcggagc acgcaacgcg acgacgcgcc tctcgacgca gccgggaagg
      361 cgctgcgccg caaaacacac gacaccgtgc gccgcgtgac gatggacatc gagcagcgcc
      421 agcagctcaa tacggcggtg tccgcgctca tggagctggt caacgagctg tactcgttca
      481 gcgaggagac ggcgacggga agcccggctc ggcacgcgac agccggagcg gcgagcgcca
      541 cgcaagagcg cgccgaggtc gtgaccgtcg tcgccgaggc gctcgatgcc ctggttcgga
      601 tgctcgcgcc gtttgctcct cacaccggcg aggagctgtg ggagcgtctt gggcatcggg
      661 gaggcctgtc ggcggcacgg tggccggcgt tcgacgccgc ggtcgcaaag gccgagcaga
      721 tcgtgattcc cgtgcaggtg aacggcaagg ttcgcagccg cctgaccgtt cccgccgaca
      781 ctaccgaggc cgagctcgag aggctggccc tcgccgatcc gcccgtgatg gcgcatactc
      841 acgggaagac cgttcggaag gtcgttgtgg cgaagggcag gctggtgtct gtggtcgttc
      901 agtaggttcc gagtgttcac aagattcagc gggttcacgg tcatcgcgat cctcgtgtcc
      961 atgagtgtcg gctgcgggta cgcgctcgcg ggtcgaggct cgttcctccc ggactacatc
     1021 aagagagtgg ggatcccgac gttcgagaac cgcagcaacg tgccggatgt cggtcaggtg
     1081 ctcacccgga aggtccgcga cgagttcatc ggccgaggca agtacgaggt caccaacgag
     1141 acggcgggat cggatgccgt gctgacgggc gaagtcctcg gcattacgct tcagccgatc
     1201 ggactcgaca gccagacgca gctcgcgtcg aggtacctgt tcacgctgac ggtgaaggtg
     1261 tcgttcacgg attcgaagac cagccaggtc ctctggtcga acgacgcgct gatcttccgc
     1321 gaggagtacg agctcggggc gcgcgccagc agcgtgacgg ccggcccggc cttcttcgac
     1381 caggaacgaa gcctgctcga tcgcctgggc ggcgacgccg cgcggtccgt cgtgacgtcc
     1441 attctcgaag ccttctgagc ggaatgccca gactgagtct ttcagagctt cgcaaacaga
     1501 ttgcgtccgg ccagacgcgg ccgctctatg ccctcatcgg cgcggatgac gccgagaagt
     1561 ccgcgatcgc ggcggagttc ggcgacatgg tggacgaagg gctgcgggct ttcaacgtcg
     1621 agcgcatgta cggcggcgac acgaccgtga aggacctcgc cgacgcggcc tgcaccctgc
     1681 cgatgctggc cacgcggcgg gtggtcatcg tcctggaggc cgaaaagctg ctcgccccga
     1741 agcgcagcag ccaggcggtc gaggaagagc aggaacgact ggaagcgttt ctcgagtcgc
     1801 ccccggatca cgcgtcggtc gtcttcgtct gcggcctgct cgacatgcgc aggcgcgtcg
     1861 tcaagcaact cctgaagcag gctcagatcg tcgactgcgg cagcatcgag aacgaggctg
     1921 atgccgaacg ctgggtcagg gcgagagcag cgcgagacaa cgtggcgctc gatcccgccg
     1981 ccgcgcgcgc gctggtggag cggaccggcc ccgatctcgt tcgcctgcgc gcggggctgg
     2041 agcgcgtcgc gctgtttgcg atgggcagag ccgtcacgcg cgacgacgtg cgcctggccg
     2101 tggctccggg cccggaatca caggccgact tcgggatcgc caaggcgatc tggcggaacg
     2161 acgtccgcga cgcgttgaaa gagctggggc tgtcgctcga ggccgggtcg ccacccttca
     2221 tggtgcttgg gcagctcagg gtcgccgcgg agaagcagcc ggcgccgcag gtgaaga
//
LOCUS       DBNGSH010000009         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3056915, whole genome shotgun sequence.
ACCESSION   DBNGSH010000009 DBNGSH010000000
VERSION     DBNGSH010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3056915"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2481)
                     /gene="acnA"
                     /locus_tag="AC16CY_00200"
     CDS             complement(<1..2481)
                     /gene="acnA"
                     /locus_tag="AC16CY_00200"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR3352222.1"
                     /translation="MPSLNSFGTRTEIRVGGQPVQIYSLPALEKAGFPGVARLPYSMK
                     ILLENLLRREDGAFVKADDVRAVAQWDASAKVEKEISFMPARVLLQDFTGVPCVVDLA
                     AMRDAVIELGGNPDRVNPLQPVELVIDHSVQVDYFGRKDAFALNAELEFSRNRERYGF
                     LRWGQRAFNNFKVVPPDTGIVHQVNIEHLARVVFSQTIDGVTVAYPDTLVGTDSHTTM
                     VNGLGVVGWGVGGIEAEAAMLGQPSSMLIPRVVGFRLTGALPRGTTATDLVLTITEIL
                     RKHGVVGTFVEFFGEGLGALTIADRATLGNMCPEYGATVAIFPIDEMTLDYLRLTGRD
                     SAQVTLVEQYAKAQGLFRTKGAPDAVYSEVVDLDLATVEPSLAGPKRPQDRVSLSKAK
                     SAFQTALPTMQVVSKGGSGTATSARTAVAVADPVVDALDHGAVVIAAITSCTNTSNPS
                     VMIGAGLLAKKAVEKGLTRKPWVKSSLAPGSIVVTEYLNAAGLTPYLDELGFNLVGYG
                     CTTCIGNSGPLPDEIASAVREKNLVVCSVLSGNRNFEGRIQQDVRANYLASPPLVVAY
                     AIAGWITTDITTDPLGHDASGKPVYLKDVWPTEREIQDTMMRTVTADMFRRTYADVYR
                     GDERWRNLPVPEGSRYAWDADSTYVRKPTFLEGMTMAVAPLKDIEGARVLAVLGDSVT
                     TDHISPAGSIKADSPAGKYLVAKGVKPADFNSYGARRGNHEVMTRGTFANVRLRNQMA
                     PGTEGGWTRYQPGGDQMAIYDASMKYQQAGVPLLVFAGKEYGSGSSRDWAAKGTFLLG
                     VKAVIAESFERIHRSNLVNMGVLPLEFKP"
     gene            complement(2578..>2830)
                     /locus_tag="AC16CY_00205"
     CDS             complement(2578..>2830)
                     /locus_tag="AC16CY_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352223.1"
                     /translation="LVPFVSAGAGYQRQRHEGETLIEGGGLFYAGGGVTHWLSVRKQG
                     TVKAIGLRGDARLYFLSGGITPDDMRTQAAFAGSFVMVF"
BASE COUNT          453 a          966 c          928 g          483 t
ORIGIN      
        1 cggcttgaac tcgagcggca gcacccccat gttcacgagg ttgctgcggt ggatgcgctc
       61 gaacgactcg gcaatcaccg ccttcacgcc gagcaggaac gtgcccttgg cggcccagtc
      121 gcgtgacgat cccgatccgt actccttgcc cgcgaagacg agcagcggca ctccggcctg
      181 ctgatacttc atggacgcgt cgtagatcgc catctggtcg ccgccgggct gatacctcgt
      241 ccagccgcct tcggtgccgg gcgccatctg attgcggagc cggacgttgg cgaacgtgcc
      301 ccgcgtcatc acctcgtgat tgccgcgccg cgcgccgtac gagttgaagt cggcgggctt
      361 cacgcccttc gcgacgagat acttccccgc cgggctgtcg gccttgatcg atccggcggg
      421 cgagatgtga tcggtcgtca cgctgtcgcc gagcacggcc agcacgcgcg cgccctcgat
      481 gtctttcaag ggcgccaccg ccatcgtcat tccctcgagg aaggtcggct tgcggacgta
      541 ggtcgagtcc gcgtcccacg cgtagcggct gccctcgggc acgggaaggt tccgccaccg
      601 ctcgtcgccg cggtagacat cggcgtaggt ccggcggaac atgtcggcgg tcaccgtgcg
      661 catcatcgtg tcctggatct cgcgctcggt cggccagacg tccttgaggt agaccggctt
      721 gccggatgcg tcatgaccga gcggatcggt ggtgatgtcg gtcgtgatcc agcccgcgat
      781 ggcgtaggcg accacgagcg gcggtgacgc caggtagttg gcccgcacgt cctgctggat
      841 gcgcccttcg aaattgcggt tgccgctcag cacggagcac acgacgaggt tcttctcgcg
      901 caccgctgat gcgatctcgt ctggcagcgg cccgctgttg ccgatgcaag tggtgcaccc
      961 gtagccgacc aggttgaacc ccagctcgtc gagatacggc gtcaggccgg cggcattcag
     1021 gtattcagtc acgacaatcg agccgggcgc caggctgctc ttgacccagg gcttgcgggt
     1081 cagccccttc tccacggctt tcttcgccag caggccggcg ccgatcatca cgctcgggtt
     1141 cgacgtgttc gtgcagctcg taatcgcggc gatcacgacc gcgccgtggt cgagagcgtc
     1201 gaccacggga tcggccaccg caacagccgt acgcgcggac gtggccgtgc ccgacccgcc
     1261 cttcgacacg acctgcatcg tcggaagcgc cgtctgaaag gccgacttcg ccttcgacaa
     1321 cgacacgcga tcctgcggac gcttcgggcc ggcgaggctc ggctcgaccg tcgccagatc
     1381 gaggtcgacg acttccgagt agaccgcgtc gggagcgccc ttggtccgga acagcccctg
     1441 cgccttcgca tattgctcga cgagcgtcac ctgcgcgctg tcgcgcccgg tcaggcgcag
     1501 gtagtcgagc gtcatctcgt cgatcgggaa aatcgccacc gtggcgccgt actcggggca
     1561 catgttgccg agggtggcgc ggtcggcgat cgtcagcgcg ccgagcccct cgccgaagaa
     1621 ctcgacgaac gtgccgacga cgccatgctt gcggaggatc tcggtgatcg tgagcacgag
     1681 gtcggtggcc gtggtgcccc tgggcagcgc gccggtcagc cggaatccca cgactctcgg
     1741 gatcagcatc gagcttggct gccccagcat cgcggcctcg gcctcgatgc cgcccacgcc
     1801 ccagccgacg accccgagcc cgttgaccat cgtcgtgtgc gagtcggtgc cgacgagcgt
     1861 gtcgggatag gcgaccgtga cgccatcgat cgtctgcgag aagacgacgc gcgccaggtg
     1921 ctcgatgttg acctggtgca cgatgccggt gtcgggcggc acgaccttga agttgttgaa
     1981 cgcgcgctgc ccccatctca ggaaaccgta ccgctcccgg ttccgcgaga actcgagctc
     2041 ggcgttgagc gcgaaggcat ccttgcggcc gaagtagtcc acctgcaccg agtggtcgat
     2101 gacgagctcg accggctgca gcggattgac gcgatcgggg ttcccaccga gctcgatgac
     2161 cgcgtcgcgc atcgccgcga gatcgacaac gcagggcacg ccggtgaaat cctggaggag
     2221 gacccgcgcc ggcatgaacg agatctcttt ctcgaccttc gccgacgcgt cccactgcgc
     2281 cacggcccgc acgtcgtcgg ccttgacgaa cgcgccgtcc tcgcgccgca gcaggttctc
     2341 cagcaggatc ttcatcgaat acgggagacg cgcgacgccg ggaaaaccgg ccttctcgag
     2401 ggcgggaagg ctgtagatct gtaccggctg tccgccgacg cggatctcgg tgcgggtacc
     2461 gaagctgttc aggctgggca tggctcttcg atgatatcag gtgcgatccc gatgcttgtc
     2521 ggtgcggcgg tgcgaacctg acgcgaatcg gtgcgagcct gatcgggatc gcaccgacta
     2581 aaagaccatt acaaaacttc cggcaaacgc cgcctgtgtg cgcatgtcgt cgggcgtgat
     2641 gcctcccgag agaaagtaga gccgcgcgtc gccacggagg cctatcgcct tcaccgtgcc
     2701 ctgcttgcgc acgctgagcc agtgcgtcac gccgccgccg gcgtagaaca ggccgccgcc
     2761 ctcgatcaat gtctcgccct cgtgacgctg gcgctgatat cccgctcccg ccgacacgaa
     2821 gggcaccagg
//
LOCUS       DBNGSH010000010        10769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3396530, whole genome shotgun sequence.
ACCESSION   DBNGSH010000010 DBNGSH010000000
VERSION     DBNGSH010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3396530"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="AC16CY_00210"
     CDS             <1..945
                     /locus_tag="AC16CY_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3352224.1"
                     /translation="PDVLLITPLIMSGSQQPDFVKSAKALGVPVVFPVFSWDNLSTKG
                     LVHVLPDRVLVWNDRQRREAIEMHQVPDPYITVTGAPRFDDFFGMRPQMSRQRFCDEY
                     GFDATQPIVLYLCSSEFVARNKWVEAGREIDFVRTWTGRLRQDPSLRGCNILIRPHPR
                     ERAQWTGFEAPERVAVALPQAMNADPSLFEALHHSAVAVGLNTSAQLEAAVAGKPVLT
                     VLVPEFAAGQQGTLHFSYLLKEQGGFVELAPDMETHLRQMASAVAGEYDPQAIRRFVE
                     SFLRPRGIDRPASPIMVEAIEAVVDERRARAAAGHATS"
     gene            942..3470
                     /locus_tag="AC16CY_00215"
     CDS             942..3470
                     /locus_tag="AC16CY_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352225.1"
                     /translation="MSAADTRFTLRRVGPPAVILCGFAVVVLYLLRELLVDSGRAPGR
                     DSVNLHVWEVYTRSVLAAGRLPFWNPFHFAGTPHLADPQTTVFYPPAVLLRWISPPQV
                     FLAWMAALHIWITGAGALFLARVIGLGWITATAVALAAMLGGSTGGYLHNGHLLPVYV
                     VSWMPWAIALSMLSLRRDTVWPHPGLVLVMVLQFLVGYLQVSVYFAGAVCLWFLLSAA
                     WPERQRRHAAPRALAQLAILGALALGVSAFQLFPTARLALESARSFGIPYEESIEDAW
                     RWRSFTKLFFPFHALPLETPHRYLTDAGSYAGWALTALAPMAFLDRNRRRIAVMLGAL
                     LVAALALAIVDLPFYRLLHELVPGFRRPSRVLVIATVSLAMLGGLGLERFLTLAAEKR
                     RRPLAAGVSIAVAAVAGAALAIGAPDGFLRPPAPVWPWLPFIAAGGVVAAFAAASRTW
                     IRTAAAVVLMVVAIDVVGFTAGAPTTVPVEPPATIRAWMGQPSAGRALSTCEHRIGAS
                     EMLRIGQPGLDGMAGIHLGSYSAWAFLARFGNAPLWDGVLRHMNTEGPLPARRDQIDL
                     ANVSVIFSCRPLDEPALTLVSEADEAYVYRNEAAWPRGVWTCEGPAMTEATAITQLLR
                     SRDDPEKPSVHINVRWASGVDDGQRRQVEQRFGLANGVVDEGRTWRYAATDPSPANML
                     ALSADPAVEDTHGFDRLTGNVHTPVVVVPGGRTDRADQVVVGGAPCSAEGTVTVLEQD
                     RSDGLFVAEVDAPVPGLVFMSEPFYVERHAFVDGRRVTPLIANLAFTAVPIGAGRHRV
                     ELRYVPESFYVGLGTTGLTLAGWTGLVWLTRSRTSVRVKGREGV"
     gene            3475..4437
                     /locus_tag="AC16CY_00220"
     CDS             3475..4437
                     /locus_tag="AC16CY_00220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3352226.1"
                     /translation="MKYSVVVPVYRNEATLPELLRQLAVMQEPYAGSLEVVFVIDGSP
                     DNSHALLKAMLPEMPFSSQLIAFSRNFGSFAAIRAGLAHATGDFAAVLAADLQQPVSS
                     VRQFFDALERGADIAVGQRASRDDPWLSRTAATMFWRLYRRFVQPQMPPGGMDSFGCT
                     RLVRDVLVSLPEANSALTGLLFWVGYRREEVTYPRLARASGRSGWSLGRKTRYAFDTT
                     FAFSDLPITIMIIAGAVGISASVAVGATVLVAWAAGAIDVRGYTPLLLAVLFAFSTTL
                     LALGIVGGYVWRIFENTKGRPTYLVSSVERIAPAVHATATARPV"
     gene            4549..5511
                     /locus_tag="AC16CY_00225"
     CDS             4549..5511
                     /locus_tag="AC16CY_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HIR3352227.1"
                     /translation="MSSDLMLADVDEKHVVPNATAPSYRKRILALLAHTRPSFMHVQS
                     DYEVRAVSRLRDEIGALGVRLFLPAAQTVDNCVDKEKSYCIWAAAGLPVPRTILLNTP
                     DDLKQAFGSLGEKIWVRATEGGGGRGALPTTSFEFARAWIDEYDGWGSFTASELLTDR
                     SVTWQSIWYEGELVVAQARRRLKWNYGNRTLSGVTGITGVGETCSDADVTRTALDAIH
                     AIDSRPHGVFCVDMTYDRGGVANPTEINIGRFFTTCYFFTKAGVNFPEIYCDLGLEGR
                     FPSLDRKINPLPDGLVWIRGMDVTPVLTTREELAKLEAGWGDGD"
     gene            5504..6436
                     /locus_tag="AC16CY_00230"
     CDS             5504..6436
                     /locus_tag="AC16CY_00230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3352228.1"
                     /translation="MTRTFAVTGARGFIGAATVRKLAGMPGTQVVAVARLRPTNAADE
                     NVQWIESALEELRPSHWQRAESAMLDAVIHLAAFTPKNAAEHDRAPEIINANVVGMQT
                     LLASLPHPPRRLVFCSTLDVYSRRALEHVLDERSPVGPTGLYGLSKLLGEGLAEAYAR
                     SAGTEHVTLRMGHVYGPGEERYAKLVPSTIRSVLAGKPPRIAGDGSDRRDLLYVDDAA
                     EAIARSCSAALDGVRTINVARGESYSILDVVNTIAELVGYRGATERLPRSADAHSTVF
                     DTSLMTRVLGAWTCVPLAEGLKREIAQFQGHLVT"
     gene            6433..8409
                     /locus_tag="AC16CY_00235"
     CDS             6433..8409
                     /locus_tag="AC16CY_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352229.1"
                     /translation="MRTSRFNSFGGVLLALLAFSWHLFLITSATNDNFAHLTFAQQWL
                     AGDWPVRDFFDHGWGLQYALSAAALAIGGERLISEAVVVGAAWAFSTWLVFQIVRRIS
                     GSSGSAVLAAVLLIVSGARGYSYPKGIVYAVAAALWWEYVRAPTTARIVGFGAWAAVA
                     FYWRPDHGIYVAVAVALAAVVAHGPRMAAIARSSLAGATMLALIAPFLLYVHLTVGLP
                     EYVQTGAVAARYEHTTHGPHQWPLIRFSAGLFTVESADSYAPTIGLRWTAGSLDNDRG
                     RVIARYGLTVLSSEGDSVRVRLSERSIPDIRALIREPIVEDTAGIERSSGTLAPSSWS
                     AWQRLRFLHSSLRVQVLPALDAQARASEFTVALFYALPIVLACAARRISARLPGLATP
                     GRLVGFALFALLVDLAMLRMPFRARAPDAVVLSAIVFGCCVAWMWRAVPPPGSLRTLF
                     RAAAIALVLAVSASVAGAGEFGDRLGGLAAQATSWQRAGAAAGSIYRELMSSPPLQAY
                     VDRRARFSLRLAAYVRECVPPSDRLLILWFEPELYYYSDRLMAHRHLAFAPAWASLSH
                     EQRMSLDKVERFSPPIVLARRSALEDQARASYPSVVGYVERQYQLASTVLDDGEEYMI
                     FARRDRPASRTFGPHNWPCFTSEASQWSRVGRAL"
     gene            complement(8427..9143)
                     /locus_tag="AC16CY_00240"
     CDS             complement(8427..9143)
                     /locus_tag="AC16CY_00240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3352230.1"
                     /translation="MNRTAEHDRLADIAADSWYGKGVNAATVAYSVRIFSRHWRPGSC
                     LELGPAEGLATRSLVEHFDDLTCVDGAEVFCRDLRARYPKIAVECALFEDYEPRKTFD
                     NIVLGHVLEHVDDPVAVLSRARGWLSANGRVFASVPNARSFHRQMGVVMGLLATEHDL
                     NDVDRHHGHRHVYDPESFRRHFAAAGLRVELFGGYWIKTVPNHQIEAHWTPEMIEAAM
                     VVGERYPDVAAEIYVIAGRR"
     gene            9269..9452
                     /locus_tag="AC16CY_00245"
                     /pseudo
     CDS             9269..9452
                     /locus_tag="AC16CY_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859754.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
     gene            complement(9433..10128)
                     /locus_tag="AC16CY_00250"
     CDS             complement(9433..10128)
                     /locus_tag="AC16CY_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019362495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR3352231.1"
                     /translation="MTSTGRKLIIVGAGETAQIAYEYFTHDSPHAVIAFAVEGEYLTT
                     RELYGLPVIPLDTLVERYPPDEFGAYVAISYNSLNRVRRRLFGLVKDMGFRCESYVSS
                     RALVWPNVAVGENCFVFESCVVQHHAQLGNDITMWAGATIAHRSVIGNDCFIASGAVI
                     NGLTRVGERCFIGANSSIADQISIADDSVIGMGAVVTQSIEEPGRVWAGNPARRLERS
                     ALETFGVVKPPTA"
     gene            complement(10125..>10769)
                     /locus_tag="AC16CY_00255"
     CDS             complement(10125..>10769)
                     /locus_tag="AC16CY_00255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3352232.1"
                     /translation="RGAARITIVSPPFAHGPELSHLVFLALHNRGYRASPHSLNQSVR
                     LDDRPFSARVDADGRRRLLRARELGLEARQLTSLSELSDAYQLIHGSMCRKGYTLSMS
                     WDRIREMAEIFGDRIHGFGVFRGQEPAAASITIRVSRHAVYVFCWADAAGFSGVSPIV
                     LLAETIYEHARAEGARWMDIGSSTNDGAPNYGLIHFKRNLGCEASLKLAYSKSL"
BASE COUNT         1638 a         3526 c         3632 g         1973 t
ORIGIN      
        1 cccgacgtcc tgctgatcac gcccctcatc atgagcggtt cgcaacagcc ggatttcgtc
       61 aagagcgcca aagcgctcgg cgtgccggtc gtgtttcccg tgttcagctg ggacaacctg
      121 tccaccaagg ggctcgtgca cgtgcttccg gatcgcgtcc tggtctggaa cgaccgccag
      181 cgccgcgagg ccatcgagat gcaccaggtg cccgacccgt acatcacggt cacaggggcg
      241 cctcgtttcg acgacttctt cggaatgcgg ccgcagatgt cgagacagcg gttctgcgac
      301 gagtacggat tcgacgccac ccagccgatc gtcttgtacc tgtgttcgtc ggagttcgtc
      361 gcgcgcaaca aatgggtgga ggcgggacgg gagatcgact tcgtccgcac gtggaccggc
      421 cggctgcgcc aggatccttc gctgcgcggg tgcaacatcc tcattcgccc ccacccccgt
      481 gagcgggcgc agtggacggg cttcgaggcg ccggaacgcg tcgccgtcgc gcttccgcag
      541 gcgatgaacg ccgatccctc gctcttcgag gcgcttcacc acagcgccgt cgccgttggc
      601 ctgaacacga gcgcgcagct cgaggcggcc gtcgccggaa agccggttct caccgtgctc
      661 gtgcccgagt tcgcggcggg gcagcagggc acgctgcact tctcctacct gctcaaggaa
      721 cagggcggct tcgtcgagct ggcgccggac atggagacgc acctgcgaca gatggcttcg
      781 gccgttgccg gtgagtacga cccgcaggcc atccgccgct tcgtcgaatc gttcctccgg
      841 ccgcgcggga tcgatcgtcc ggccagtccg atcatggtgg aggcgatcga ggcggtggtc
      901 gatgagcgca gggccagggc tgcagccggt cacgcgacgt catgagcgcc gcggacaccc
      961 gcttcacact ccggcgcgtg gggccgccgg cggtgatcct ctgcgggttc gcggtcgtcg
     1021 tcctctacct gctccgcgag ttactcgtgg acagcgggcg ggcgcccggg cgcgacagcg
     1081 tcaacctgca cgtctgggag gtgtacaccc ggtcggtgct tgcggccggc cggctgccgt
     1141 tctggaatcc cttccacttc gcgggtaccc cgcacctggc cgacccgcag acgaccgtgt
     1201 tctatccacc cgcggtcctg ctccgatgga tctcgccgcc ccaggtcttt ctggcctgga
     1261 tggcggccct ccacatctgg attacgggcg cgggcgcgct gttcctggcg cgcgtcatcg
     1321 gcctggggtg gatcacggca acggccgtcg ctctcgccgc gatgctcggc ggcagcaccg
     1381 gcggttacct ccacaacggg catctgctgc ccgtttacgt cgtctcgtgg atgccatggg
     1441 cgatcgcgct gagcatgctc tcgcttcgcc gcgacacggt ctggcctcac cccgggctgg
     1501 tgctcgtgat ggtgctgcag ttcctcgtgg ggtatctgca ggtcagcgtc tatttcgccg
     1561 gggccgtgtg cctgtggttc ctgctcagcg ctgcgtggcc cgagcggcag aggcgccatg
     1621 cggccccgcg tgctctcgcg caactggcca tcctcggcgc gctcgcgctc ggagtgagcg
     1681 cgttccagct gttcccgacg gcaaggctcg cgctcgaatc agcccgcagc tttggaatcc
     1741 cgtacgagga gtccatcgag gacgcctgga ggtggcggag cttcacgaag ctgttcttcc
     1801 cgtttcacgc ccttcctctg gagaccccgc atcgctacct gaccgacgcg ggctcgtacg
     1861 ccggctgggc tctcaccgcc ctggcgccga tggcgtttct cgatcggaat cgtcgtcgca
     1921 tcgcggtcat gctcggcgca ctgctcgtcg ccgccctggc tctggcgatc gtggatctgc
     1981 cgttctaccg tctgctccac gagctggtgc ccgggtttcg cagaccgagc cgcgtactcg
     2041 tcatcgcgac ggtgagcctg gcaatgcttg gcggcctcgg tctcgagcgc ttcctgactc
     2101 tggccgccga aaagaggcgg cgtccgctgg ccgccggcgt gtcgatcgcg gtcgcagcgg
     2161 tcgctggcgc ggcgctcgcc atcggggcgc ccgacggctt cctcaggccg ccggctccgg
     2221 tatggccgtg gctcccattc atcgcggctg gcggcgtggt cgcggcgttc gcggcggcat
     2281 cacgcacctg gatacgaacg gccgcggcgg tcgttctgat ggtggtggcg attgacgtcg
     2341 tcgggttcac tgccggcgcc ccaacgacgg tgcccgtgga gccgccggcg acgatcagag
     2401 catggatggg acagccctcc gcggggcgcg ccctctcgac gtgcgagcac cggatcggtg
     2461 ccagtgagat gctgcgcatc ggccagcccg ggctcgacgg catggccggc atccaccttg
     2521 gctcgtactc cgcctgggca ttcctggcca ggttcgggaa tgcgccgctg tgggacggcg
     2581 tgcttcgcca catgaacacc gaaggtccgc tccccgctcg ccgcgatcaa atcgatctcg
     2641 ccaacgtgtc ggtcatcttt tcgtgccgtc ctctcgatga gcccgcgttg acgctcgtgt
     2701 cggaagcaga cgaggcctac gtctaccgga acgaagcggc gtggccgcgg ggcgtctgga
     2761 cgtgcgaggg cccggcgatg accgaggcga cggcaatcac gcagctgctg cggtcgcgcg
     2821 acgacccgga gaagccgtcg gttcacatca acgtgaggtg ggcgtccggc gttgacgacg
     2881 gccagcgcag gcaggtcgag cagcgctttg gtcttgcaaa cggagtcgtg gacgaaggcc
     2941 gcacgtggcg ctacgcggca acggatccat ccccggcgaa catgctggcg ctcagcgccg
     3001 acccggcagt cgaggatacg cacgggttcg acaggttgac cggcaacgtc cacacaccgg
     3061 tcgtggtcgt gcccggcggc cgcacggatc gagcggatca agtagtggtg ggcggggccc
     3121 cgtgctccgc ggagggcacc gtgacggtgc tcgaacagga ccggtccgac ggcctcttcg
     3181 ttgccgaggt ggatgcaccg gtccccggcc tcgtgttcat gagcgagccg ttctacgtcg
     3241 agcggcacgc gttcgtggac ggacgacgag tgacgccgct gatcgcgaac ctcgcgttca
     3301 cggccgtgcc gatcggcgcc ggcagacatc gcgtcgagct gcgctacgta ccggagagct
     3361 tctacgtcgg cctcggtacg accggcctga cgctggcggg ttggacgggc ctggtgtggc
     3421 tgaccagatc gagaacgtcc gtccgcgtga aaggacgcga aggggtctga ccccatgaag
     3481 tactccgtgg tcgtgccggt ctatcggaac gaggcgacgc tgcccgagct gctccgccag
     3541 cttgcggtga tgcaggagcc gtacgcaggt tcgctcgagg tcgtgttcgt catcgacgga
     3601 agccccgaca actcgcacgc cctgctcaag gcgatgctgc cggagatgcc gttttcgtcg
     3661 cagttgatcg ccttcagccg caacttcgga tcgtttgcgg ccatccgggc cgggctggct
     3721 cacgcgacgg gcgacttcgc cgccgtcctc gcggccgatc tgcagcagcc ggtctcgtcc
     3781 gtccgacagt tcttcgacgc gctcgagaga ggcgccgaca tcgccgttgg ccagcgcgcc
     3841 tcgcgcgacg atccgtggct gtcgcggacg gccgcgacga tgttctggcg gctctaccgg
     3901 cgtttcgtcc agccccagat gccgcccgga ggcatggatt cgttcggatg cacgcggctt
     3961 gtccgtgacg tgctcgtctc gctcccggaa gccaacagcg cgctcacggg cctgctcttc
     4021 tgggtcgggt accggcgcga agaggtgaca tatcctcgtt tggccagggc cagcggccgg
     4081 agtggatggt cgctcggcag gaagacgcga tatgccttcg acacgacctt cgcattttct
     4141 gacctgccta tcacgatcat gatcatcgcc ggagcggtcg gaatttcggc ctcggtcgcg
     4201 gtcggcgcga ccgtcctcgt cgcctgggcg gccggcgcga tcgatgtccg ggggtacaca
     4261 ccgctgctgc ttgccgtgct ctttgccttc tcgaccacgc tgctcgcgct cggcatcgtg
     4321 ggcggctacg tgtggcgcat cttcgagaac acgaagggaa gaccgacata cctcgtgagc
     4381 tcggtggaac gcatcgcgcc tgcggttcat gcgacggcca ccgcaagacc tgtctgaagc
     4441 accatggctc gcatacacat aggtggcgcg ggtggggctc cctccaacaa tgtcattcgt
     4501 tcgttgcgtg agtcctcgcg agggcactat ctcatcggag catcgtcagt gtcgtccgat
     4561 ctgatgctgg ccgatgtcga cgagaagcat gtcgttccga acgcgactgc gccgagctac
     4621 cggaaacgaa ttctggcgtt gctggcgcac acgcgcccgt cgttcatgca cgtgcagagc
     4681 gactacgaag tgcgcgccgt ctcacggtta cgcgacgaga tcggcgcact cggcgttcga
     4741 ttgttccttc cggcggcgca aacggtcgac aactgcgtgg acaaggaaaa gtcctactgc
     4801 atctgggccg ccgccggcct acccgtgccg cgaacgattc ttctgaacac acccgacgac
     4861 ctcaagcagg cattcggttc gctgggcgag aagatatggg ttcgcgccac ggagggagga
     4921 gggggacgcg gagcgctgcc gacgaccagt ttcgagttcg cgagggcgtg gattgatgag
     4981 tacgacggat ggggctcttt cacggcgtcc gagctgctca cggatcgctc ggtgacctgg
     5041 caatcgatct ggtatgaagg cgagttggtc gttgcccagg ccaggcgtcg gctcaaatgg
     5101 aattatggca atcgcacgct ttctggagtg accggtataa ccggcgtggg agaaacgtgc
     5161 tccgatgcgg acgtgactcg tacggcgctg gatgccatcc acgcaatcga ctcccggccg
     5221 cacggtgtgt tctgtgtgga tatgacgtat gaccgcggcg gcgttgccaa ccccactgag
     5281 atcaacatag gccgattctt caccacgtgc tatttcttca cgaaagcagg cgtcaacttt
     5341 ccagagatct attgcgacct tggcctcgaa ggacgattcc catctctcga ccggaagatc
     5401 aatccccttc cggacgggct cgtgtggatt cgcggcatgg acgtcacgcc ggttctcacg
     5461 acgcgtgagg aattggcgaa actggaggcc ggctggggcg acggtgacta ggactttcgc
     5521 ggtcaccggt gcgcgcggat tcattggcgc tgcgactgtt cgcaagctgg ccggcatgcc
     5581 gggcacccag gtcgtcgccg ttgcccgatt gcggccgacc aatgccgcgg acgagaacgt
     5641 tcagtggatt gagagcgcct tggaagagct gaggccgtcg cactggcaac gcgccgaatc
     5701 ggcgatgctc gacgctgtca ttcacctggc ggccttcacg ccaaagaacg ccgcagaaca
     5761 cgaccgcgcc ccggagatca tcaacgcaaa tgtcgtcggc atgcagacct tgctcgcgag
     5821 cctgcctcac cccccccgca ggctcgtgtt ctgcagcacg cttgacgtct actcacgccg
     5881 ggcgctcgag catgtgctgg acgaacggag cccggttggc cccaccgggt tgtacggctt
     5941 gtccaagctg cttggtgagg gcctggccga ggcgtatgct cgatcagccg gcaccgagca
     6001 cgtcacgctc cgcatggggc acgtgtatgg tcctggcgag gaacgctacg cgaagctggt
     6061 tccctcgacg atacgcagcg tgctcgctgg caagcccccg cgaatcgccg gcgatggcag
     6121 cgacaggcgt gacttgctgt acgtcgatga cgccgccgag gcgatcgccc gatcatgcag
     6181 tgctgcattg gacggagtac gcacaatcaa cgttgcgcgg ggcgagtcgt actcgatact
     6241 cgacgtcgtc aacacgattg cggaactggt cggctaccga ggggcaacgg agcggttacc
     6301 gcgttccgcc gacgcccact cgaccgtgtt cgacacgtcg ctcatgacgc gtgtcctcgg
     6361 cgcgtggacg tgtgttcctc tggctgaagg cctcaagagg gagatcgcac agtttcaagg
     6421 acatttagtg acttgaggac gagccggttc aacagcttcg gcggtgtcct gctcgcgctg
     6481 ctcgcgttct catggcacct tttcctgatc acgtccgcaa cgaacgacaa cttcgcgcac
     6541 ctcacgttcg cgcagcaatg gctggcgggc gattggcccg ttcgagattt cttcgatcac
     6601 ggctgggggc tccagtacgc gctcagcgcg gcggcgctgg cgatcggcgg cgagaggctc
     6661 atttccgagg cggtcgtcgt cggcgcagcg tgggccttca gtacctggct cgtgtttcag
     6721 atcgtgcggc gcatttccgg atcctccggc tcggccgttc tggcggcggt cctgctgatc
     6781 gtgtccggcg cgcgaggcta ctcctatccc aaaggcatcg tctatgccgt tgccgccgcg
     6841 ttgtggtggg agtacgttcg agcgcccacg accgcgcgaa tcgtgggttt cggggcgtgg
     6901 gcggcggtcg ccttctactg gcggccggat cacgggatct atgttgccgt ggcggtggcc
     6961 ctggccgccg tcgttgcgca cggcccccgg atggcggcca tcgctcggtc gtcgctggcc
     7021 ggcgccacga tgctggcttt aattgctccg ttcctcctgt acgtgcatct gacggtcggt
     7081 ctgccggagt acgtgcaaac cggggcggtg gcggcccggt acgagcacac gactcacgga
     7141 ccccatcagt ggcctctgat ccggttctcc gccggcctct tcacggtcga gtccgccgac
     7201 agctacgcgc cgacgatcgg ccttcgatgg accgccggga gcctcgacaa cgatcgcggc
     7261 cgcgtcatcg cgcgctacgg cctgaccgtg ctctcgtcgg aaggcgattc cgtccgggtg
     7321 cggctgtccg agcgatccat tccggacatc cgcgcactga ttcgcgagcc gatcgtggag
     7381 gacacagccg gaatcgagcg gtcgagcggt acgctggcgc cgtcgtcctg gtccgcctgg
     7441 cagcgcctgc ggttccttca ctcatcgctc cgtgtgcagg tgctgccggc gctcgacgcg
     7501 caggcccggg cgagcgagtt cacagtcgcg ctgttctatg cgctgccgat cgttctcgcg
     7561 tgcgcggctc gacggatatc cgcgcggttg ccgggcctgg ccactccagg gcggctcgtc
     7621 gggttcgcgc tgtttgcgct cctggtcgat ctcgcgatgc tgcgaatgcc gttccgcgcg
     7681 cgcgcgccgg atgccgtcgt cctgtcggcg atcgtcttcg gctgctgcgt cgcctggatg
     7741 tggcgcgcgg ttcccccgcc agggtccctg cgaacattgt ttcgggcggc cgcaatcgcg
     7801 ctcgtgctcg cggtgtcggc gagcgtcgcc ggagcggggg agttcggcga tcgcctcggg
     7861 ggccttgcgg cacaagccac ctcctggcag cgcgcgggcg cagccgctgg gagcatctac
     7921 cgggagttga tgtcgtcgcc gcccctgcag gcctacgtgg accggcgtgc ccgcttcagt
     7981 ctccggctgg cggcgtacgt gcgcgagtgc gtgccgccat cggaccggtt gctcatcctg
     8041 tggttcgagc cggagctcta ctactacagc gatcggctga tggcgcaccg tcatctggca
     8101 tttgccccgg cttgggccag cctgtcgcac gaacagcgga tgtcgctcga taaggtcgag
     8161 cggttctcgc cgccgatcgt cctggctcga cggtccgcgc tagaggatca ggcgcgcgcg
     8221 agctatccga gtgtggtcgg ctacgtcgag cgccagtacc agctagcctc gaccgtcctc
     8281 gacgatggcg aggagtacat gatcttcgcg cggcgcgaca ggccggcctc acggaccttc
     8341 ggcccgcaca actggccctg cttcaccagc gaggcgtcgc agtggtcgcg ggtgggcagg
     8401 gcgctctgag aatgccgggg agccggtcag cgtcgtccgg caatcacgta gatttcggcc
     8461 gcgacgtccg ggtagcgctc gccgaccacc attgcggcct cgatcatctc cggagtccag
     8521 tgcgcctcga tctgatgatt cggcacggtc ttgatccagt agccgccgaa gagctcgacc
     8581 cgtaaccccg cggcggcgaa gtggcggcga aacgattcag ggtcgtagac gtgacggtgg
     8641 ccgtggtggc gatcgacatc gttcaggtcg tgctcggtcg cgagaagacc catcacgacg
     8701 cccatctgcc ggtgaaagct gcgtgcgttg ggaaccgagg caaacacgcg tccgttcgcg
     8761 ctcaaccagc ccctcgcccg ggacagaacg gcgacggggt cgtccacgtg ctcgaggacg
     8821 tgtccgagca cgatgttgtc gaaggtctta cgcggttcgt agtcctcgaa gagcgcgcat
     8881 tcgacggcga tcttcggata gcgcgcgcga agatcccggc agaagacttc cgcgccgtcc
     8941 acgcacgtga ggtcgtcgaa gtgctcgacg agtgatcggg tggcgagccc ttcggcgggt
     9001 ccgagctcca ggcaggatcc cggccgccag tggcgggaga agatgcgcac gctgtaggcc
     9061 accgtcgccg cgttcacacc tttgccgtac cagctgtcgg cggcgatgtc cgcgagtcga
     9121 tcgtgctctg ccgtcctgtt catatcgtcg aagacgccga ttctatgtcg atcgcgcgcg
     9181 gccggagaag ggcgttttcc gggtcgccca ggagtatatt cgtggctgcc ccgcttgagt
     9241 accgttagac agttgatatg cccatagact gcgcccacgg gcggcaaacc atagagcgag
     9301 cgccgactgg cacgtggtgc ccacgctggt gaagcgcgga gcctcgatcg gcagcggcgc
     9361 ctcgatcatc tgtgggatca ccgattggtg aaggggcgat ggttggcgcg ggtgcggtcg
     9421 tgacgcggga cgtcacgccg tcgggggctt gaccacgccg aacgtctcga gcgcgctcct
     9481 ctcgagccgg cgtgcggggt tgccggccca cactcggccc ggttcctcga tactctgcgt
     9541 gacgacggca cccatcccga tgacgctgtc gtcagcaatg gagatctgat cggcgatgct
     9601 gctgttggcc ccgatgaagc agcgctcgcc gacgcgcgtc aaaccgttga tgacggcgcc
     9661 agatgcaatg aaacagtcgt tgccgatgac gctgcgatgc gcgatcgtcg cgccggccca
     9721 catcgtgatg tcgttcccga gctgcgcgtg atgctggacc acgcaggact cgaacacgaa
     9781 gcagttttcg ccgaccgcca cgttcggcca gaccagcgcc cgggaactga cgtacgactc
     9841 gcagcgaaac cccatgtcct tcaccaggcc gaagagacgg cgccgtacac ggttcagcga
     9901 gttgtacgag atcgcgacgt atgcgccgaa ctcgtccggc gggtagcgtt cgacgagtgt
     9961 gtcgagcggg atgaccggaa gaccgtacag ctcgcgggtc gtgaggtact ccccctcgac
    10021 ggcgaaggcg atgacagcgt ggggagagtc gtgcgtgaag tactcgtacg cgatctgggc
    10081 cgtttcgccg gcgccgacga tgatcagctt ccgccccgtt gacgtcacag cgacttcgag
    10141 tacgcgagct tcagggacgc ctcgcagcca aggtttcgct tgaagtggat caggccgtag
    10201 ttgggcgctc cgtcgttggt ggacgagccg atgtccatcc agcgtgcgcc ttcggcgcgg
    10261 gcatgctcgt agatcgtttc cgcgagcagg acgatcgggc tcacgcctga gaatccggct
    10321 gcgtccgccc agcagaagac atacacggcg tgccggctca ccctgatcgt gatgctcgcg
    10381 gccgcgggct cctgcccgcg aaacaccccg aacccgtgga tccggtcgcc gaaaatctcc
    10441 gccatctcac ggatgcggtc ccaggacatg gacagtgtgt agcccttgcg gcacatgctc
    10501 ccgtgaatca gctggtaggc gtcggacagc tcggacaggg aagtcagctg tcgcgcctcg
    10561 agacccagtt cgcgtgcgcg caggagccgc ctccgcccgt cggcatcgac cctggcgctg
    10621 aacggccgat cgtccaggcg aacagactgg ttgaggctat gtggtgaggc acgatacccg
    10681 cggttatgga gcgccaggaa caccaggtgc gagagctctg gcccgtgcgc aaacggcggg
    10741 gagacgatcg tgatccgtgc agcaccgcg
//
LOCUS       DBNGSH010000011         2962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3396562, whole genome shotgun sequence.
ACCESSION   DBNGSH010000011 DBNGSH010000000
VERSION     DBNGSH010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3396562"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="AC16CY_00260"
     CDS             complement(<1..873)
                     /locus_tag="AC16CY_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR3352233.1"
                     /translation="MLTLLSRSVTLKRLASQYGMATPRSFGRRFIAGERVEDAIEAAR
                     ALQARGLGVTLDFLGESVATTEQAMAAAGEYQRLIDVVVASGVERNLSLKLSQIGLAI
                     DRATCVDNMRRILDPAKRHDFFVRIDMEGSAYTEVTLEVLETLWGLDYHNIGIVLQSY
                     LYRTERDLRRMNELGVRVRLVKGAYRESASVAYQLKEDVDGAFLRQMRLLLDHGVSPA
                     FATHDEALLREIRDYAAERHVSSDRYEFQMLYGVRRDLQASLRADGYRMRVYVPFGRE
                     WFPYFMRRLGERKEN"
     gene            complement(898..2022)
                     /locus_tag="AC16CY_00265"
     CDS             complement(898..2022)
                     /locus_tag="AC16CY_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3352234.1"
                     /translation="MLDEITQAVLSREEVARYLQGTHGESGRAARERIEGYLDELKTT
                     QRYSIYRALKHPLYPILRKIERLGEHVDIVKAATRAGRVVYASNHRSHIDYLCEPLVL
                     DDNGIRPPIIAAGINLFGGPLGLIHRHVTGAIPIRRNTRDPVYLITLKAYVAEMLRSH
                     DFFFYPEGGRSYSGDMKVPKTGLLHAALQARYPGLRIIPTAVAYDLVLEDHVLARQGV
                     KRSQRAFSRELAELVRYAVGYRSRAIVAFGTPIDADGIDADSRRAVLDLAHIVMQAIG
                     RLYKVLPTAVVASAMRPSVTRRELEQRADALIDTLRASGANMGVSTGAEAVDAALEPL
                     EARGVLVVSRGRVRVRNRNVLRYYARSLEHLLKSPSHRTH"
     gene            complement(2087..2578)
                     /locus_tag="AC16CY_00270"
     CDS             complement(2087..2578)
                     /locus_tag="AC16CY_00270"
                     /inference="COORDINATES: protein motif:HMM:NF025046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HIR3352235.1"
                     /translation="MPALRYVYVLALAVWLGGMILLGTVVAPLTFQVLQASDPDTGRV
                     LAGELFGAVLNRFHVVAYGAGALLLVTLVAMAILGPRPRSFAVRAAIISSMLLVALYS
                     GLVVLREVDAIQLAAGVLPSTLPAGDARRVRFDALHQLSTRLMVFNIVGTLALLGWEA
                     REH"
     gene            complement(2586..>2962)
                     /locus_tag="AC16CY_00275"
     CDS             complement(2586..>2962)
                     /locus_tag="AC16CY_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352236.1"
                     /translation="EGIADAIRQGVYAEGPFSYNWIGRFFAHVIEPPPRVRLKSMLHT
                     QPGPLRPKRETLAGFRAYQVQYIDRLRQANGLDLAHARVRSPISAWIRIPLGSAFAAV
                     TAHERRHIWQLRKTLGMAGFPR"
BASE COUNT          485 a          999 c         1011 g          467 t
ORIGIN      
        1 gttctccttg cgttccccga ggcgccgcat gaaatacgga aaccattccc gcccgaacgg
       61 gacgtagacg cgcatgcggt atccgtcggc gcgcagcgag gcctgcaggt cccggcggac
      121 gccgtagagc atctgaaact cgtaccgatc gctcgacacg tgccgttccg cggcgtagtc
      181 ccgaatctcc cgaaggagcg cctcgtcgtg cgtggcgaac gcgggagaga cgccgtggtc
      241 gagcaggagc ctcatctggc gaaggaaggc cccgtccacg tcctctttca actgataggc
      301 caccgacgcc gattcccgat acgcgccctt caccaggcgc acacggacgc ccagctcgtt
      361 catgcggcgc agatcgcgct cggtccggta cagatacgac tgcagcacga tgccgatgtt
      421 gtggtagtcg agcccccaga gcgtttccag gacctcgagc gtgacttccg tgtacgcgga
      481 cccctccatg tcgatgcgca cgaagaagtc gtggcgcttg gccggatcca ggatccggcg
      541 catgttgtcc acgcacgtcg cccggtcgat ggcgaggccg atttgcgaaa gcttgaggga
      601 gagattgcgc tcgacgcccg acgccacgac gacgtcgatg agccgctggt actcgcccgc
      661 ggccgccatg gcctgttccg tcgtcgcgac gctctcaccc aggaaatcga gcgtgacccc
      721 gagccccctg gcctgcagcg cgcgtgcggc ctcgatggcg tcttcgacgc gctcgccggc
      781 gatgaaccgg cgcccgaagc ttcgcggcgt cgccatgccg tactgcgagg cgagccgctt
      841 gagagtcacg cttctcgaca gtaaggtgag catcggtccg gcgcggccac gccgccgtca
      901 gtgggtgcgg tgcgacggcg acttcagcag gtgttcgagc gatcgggcgt agtagcgcag
      961 cacgtttcgg ttgcggacgc ggacgcgtcc gcggctgacg accaggacgc cgcgcgcttc
     1021 gagcggctcg agcgcggcgt ccaccgcctc ggctccggtg gacacgccca tgttggcgcc
     1081 tgacgccctg agcgtgtcga tcagcgcgtc ggcgcgctgc tcgagctcgc gccgcgtcac
     1141 cgacggacgc atcgcggagg cgacgacggc cgtgggcagc actttgtaga gcctgccgat
     1201 cgcctgcatc acaatgtgcg ccaggtcgag caccgcgcgc cgggaatccg cgtcgatgcc
     1261 gtcggcgtcg atcggcgtgc cgaacgcgac gatcgcgcgc gaccggtacc cgaccgcgta
     1321 gcgcaccagc tccgcgagct cgcggctgaa ggcccgctgc gatcgcttga cgccctggcg
     1381 ggcgagcacg tggtcctcga gcaccaggtc gtaggccaca gccgtcggaa tgatccgcag
     1441 gcccgggtat ctcgcctgca gcgccgcgtg cagcagtccc gtcttgggaa ctttcatgtc
     1501 gccgctgtag ctgcggccgc cttccggata gaagaagaaa tcgtgggagc ggagcatctc
     1561 ggcgacgtac gccttgagcg tgatgagata caccgggtct ctcgtgttcc gccggatcgg
     1621 gatggcgccg gtgacgtgac ggtggatcag gccgagcggc cctccgaaca gattgatgcc
     1681 ggcggcgatg atgggcggcc ggatcccgtt gtcatcgagc accagcggct cgcacaggta
     1741 gtcgatgtgg ctccggtgat tggaggcgta gacgacgcgg ccagcccgcg tggccgcttt
     1801 gacgatgtcc acgtgctcgc ccaggcgctc gatcttgcgg aggatcgggt acagcgggtg
     1861 cttgagcgca cgatagatcg aataccgctg cgtcgtcttg agctcgtcga gatagccctc
     1921 gattcgctcg cgcgccgcac ggccgctttc accgtgtgtg ccttgcaggt accgggcgac
     1981 ttcctcccgg gagagcacgg cctgggtgat ctcgtcgagc atcgacaagc gttgactata
     2041 ccatcgacgc cgccgcgtcc cgtgccccct gccccctgct ccctgtttag tgctcccttg
     2101 cttcccagcc aagcagcgcg agcgtgccca cgatgttgaa caccatcagc cgcgtcgaga
     2161 gctgatgcag ggcgtcgaag cgcacacgcc tcgcgtcgcc ggcgggcagc gtggacggca
     2221 ggacgcccgc ggccagttga atcgcgtcga cttcccgcag gaccaccagt cctgaataga
     2281 gcgcgacgag cagcatgctg gagatgatcg cggcccgcac ggcgaagctg cgcgggcgcg
     2341 gaccgagaat ggccatggcg accagcgtga cgagcagcag ggcgcctgcc ccgtaggcga
     2401 cgacgtggaa tcggttgagg accgcgccga agagctcgcc cgcgaggacg cggccggtgt
     2461 cggggtcgct ggcctggagc acctggaacg tcagcggcgc gacgacggtg cccagcagga
     2521 tcatcccgcc cagccagacc gccagtgcga gaacgtagac ataccggagc gccggcatcg
     2581 agtcattatc tcggaaagcc ggccatcccg agcgtcttgc gcaactgcca gatgtggcgc
     2641 cgctcgtgcg ctgttaccgc ggcgaacgcc gagcccagcg gaatccggat ccaggccgag
     2701 atgggagatc gcacgcgcgc gtgcgccagg tcgaggccgt tggcctgccg caggcggtcg
     2761 atgtactgca cctggtaggc gcgaaagccc gccagcgtct cgcgcttcgg ccgcagtggc
     2821 cctggctgcg tgtgaagcat cgacttcagg cgcacccgcg gcggcggctc gatcacgtgg
     2881 gcaaaaaacc ggccgatcca gttgtaagag aacggtccct cggcgtacac gccctggcga
     2941 atggcgtcgg cgatcccctc gt
//
LOCUS       DBNGSH010000012         4596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_672, whole genome shotgun sequence.
ACCESSION   DBNGSH010000012 DBNGSH010000000
VERSION     DBNGSH010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_672"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /gene="pyk"
                     /locus_tag="AC16CY_00280"
     CDS             complement(<1..1352)
                     /gene="pyk"
                     /locus_tag="AC16CY_00280"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3352237.1"
                     /translation="MRRTRIIATLGPASSSRSVIDEMIAAGVDVFRLNFSHGTREGHA
                     SVIEVVRQAAQQAGRIVAVLQDLSGPKIRTGTLRGGAPVPLESGAELRIVVGNAPGDA
                     TCVSTTYADLPKSVHAGDTMLLDDGRIELRVVGVAGEEIRTVVVDGGTLGERKGINVP
                     GVELPTRGLTEKDIEDLRFGVRAGVDVVALSFVQSAAVLRQAREEARLAGGPEIPIVA
                     KLERPEAITRLEEILRDSDAVMVARGDLGLELPLERVPRIQKEIVRRARALGVPVIVA
                     TQVLESMRTEPRPTRAEVSDAAVAVDEGVDGIMLAGETAAGAYPVRAVQMLDLVIREA
                     ESIPPGRAEPLSSPSGQAQAVCEAAVTLAERGDARAIVAVTRSGTTARMLAALRPRVP
                     IVAVTDRADVSRRLALVWGVQPLLTDFSGDVGSAAARITADLIARGVLEPGSIVVVVN
                     V"
     gene            1576..1749
                     /locus_tag="AC16CY_00285"
     CDS             1576..1749
                     /locus_tag="AC16CY_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352238.1"
                     /translation="MTVDTWLENALRDADRRQLPALRPLLEALARSTRLLRAADWNVD
                     ASDRTAPPTGHAR"
     gene            1739..3088
                     /locus_tag="AC16CY_00290"
     CDS             1739..3088
                     /locus_tag="AC16CY_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3352239.1"
                     /translation="MHAEPKTLADLSRALEDRAITSEEATERCLSRIAERNPAINAFI
                     TVLADEATAQARGADREIAGGRRRGPLHGVPISVKDLIDLRGTPTTAASRVRQGHIAR
                     QDATVIGRLRTAGAVFIGKTNLHEFALGTTNEDSAYGPVRHPLDDSRSPGGSSGGSAA
                     SVLDGMAFASVGTDTGGSIRIPSAACGLVGLKPTIGDLPTDGIVPLSATLDHVGPICL
                     TVEDTTLLYGAMRGVLKPEYPPLRDVRGLRFGVPRPYFFDLLDTEVAARFEDACGRLR
                     SAGAILEDVLIPHTAEIATIYLHVALPEAASCHAATLESQPDDYTTNVRLRLEMGRYI
                     LAEDYVRARRGQEVLTQEVKDALAGRDCLLLPSLPIPATRLGEPMVRIGESEEPVRNV
                     TLRLTQLFNITGHPAISVPCGRTTGGLPVGAQLVGARYRTADLLQVAAAVERTLEHS"
     gene            3095..4156
                     /locus_tag="AC16CY_00295"
     CDS             3095..4156
                     /locus_tag="AC16CY_00295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3352240.1"
                     /translation="MLKKIFHSWERHLASVSKDRVVRPFEWGLDWVSHNGHDLHDPAA
                     HLGEWVRDVMRDTPAFFSVQPTRDYDFVEATPALKQQGEAGTLRFPSAFVTPHPENNV
                     VVGRWFPSASEPPVGVRRGRGRAVVVLSQWNADSEGHIGLSKLLARFGVSALRLSLPY
                     HDTRMPPELTRADYIVSSNVARTVQVCRQAVLDTRRAVAWLASQGFDRIGILGTSLGS
                     CLAMLTSAHEPLIRAQALNHVSPWFADVVWRGLSTRHVRTGLDGHIDLDLLRALWLPI
                     SPIEYLERIRDKKTLLVYARYDLTFPVDLSRLLIDEFGKRNIPHTAFALPCGHYSTGW
                     APFKYVDGFVLTKFLRNSL"
     gene            complement(4295..4540)
                     /locus_tag="AC16CY_00300"
     CDS             complement(4295..4540)
                     /locus_tag="AC16CY_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352241.1"
                     /translation="MSEDLEQVIRDDDLERGFDRVTSVAVLIGAIAAVLAGATIWLVL
                     TDPVTVASALDGGEISPLVRRLAEVIFNALAGLLDFL"
BASE COUNT          741 a         1586 c         1533 g          736 t
ORIGIN      
        1 acgttgacga ccacgacgat cgagccgggc tcgagtactc ctcgtgcgat gagatccgcg
       61 gtgatgcggg cggctgccga gccgacgtcg ccgctgaagt cggtcagcag cggctgcact
      121 ccccagacca gcgcgagccg ccgcgagacg tccgcccgat cggtgacggc gacgatggga
      181 acccgggggc gcagggccgc cagcatgcgc gccgtcgtcc cgctgcgcgt gacagccacg
      241 atggcgcgcg cgtctccgcg ctcggccagc gtgacggccg cttcgcagac ggcctgcgcc
      301 tgcccgctgg gcgaggacaa cggttccgcg cgccctggcg ggatcgactc ggcctcgcga
      361 atcacgaggt ccagcatctg gacggcgcga accgggtacg cgccggccgc ggtctccccg
      421 gccagcatga tgccatccac gccttcgtca acggccacag ccgcgtcgct gacctcggca
      481 cgcgtcgggc gcggctcggt gcgcatcgat tcgagcacct gcgtcgcgac gatgacgggg
      541 acgccgagcg cacgggcgcg gcggacgatc tccttctgga tccttggcac ccgctcgagc
      601 ggcagctcga gccccaggtc gccgcgcgcc accatcaccg cgtcgctgtc gcgaaggatt
      661 tcctcgagcc tggtgatagc ttccggacgt tcgagcttcg cgacgatggg aatctcgggg
      721 ccgccagcga gacgagcctc ctcgcgagcc tgccgcagga cggccgcgct ctgaacgaag
      781 ctcaaggcga cgacgtccac gccggcgcga acaccgaagc ggagatcctc gatgtccttc
      841 tccgtgagtc cgcgagtggg cagctccaca ccgggtacgt tgatgccctt gcgctccccc
      901 agcgtccctc catcgacgac gacggtacgg atctcctcgc cggccacgcc gacgacgcgg
      961 agctcgatcc gcccgtcgtc gaggagcatc gtgtccccgg cgtgcacgct cttcggcagg
     1021 tcggcgtagg tcgtggagac gcacgtggca tcgcccggag cgttgccgac gacaatgcgc
     1081 agctccgctc cggattcgag cggcacggga gcgccgcccc gcagggttcc cgtgcggatc
     1141 ttcggaccac tgagatcctg cagaacggcc acgatgcgtc cggcctgctg tgccgcctgt
     1201 cgcacgacct cgatgaccga ggcatgcccc tcccgcgtgc cgtgggagaa gttgagacgg
     1261 aatacgtcga cgccggccgc gatcatctcg tcgatgacgg acctgctcga cgacgcgggg
     1321 ccgagtgtgg cgatgattct cgtgcgcctc atggggacaa tggacctggg ttcagtgggt
     1381 tcagtgggtt cacggggtgc cgtgggtcct gttcaatggg ttcatcgggt tcaagggttc
     1441 agatggttcg gcgaacccat cgaacctgcg gaacccccgg aaccccttga acaggaccca
     1501 ttgaaccata tgaaccttgt gaacccactg aaccgacgag gatcgatttc cgtttagaag
     1561 tagcctagta gagagatgac ggttgacacc tggctcgaga acgcgctccg ggacgccgac
     1621 cggcgtcagt tgcccgccct tcgcccgctg ctcgaggcgc tcgcgcgctc gaccaggctg
     1681 cttcgcgccg ccgactggaa cgtggacgca agcgacagga ccgcgccccc gaccggacat
     1741 gcacgctgag ccgaagacac tggccgacct ttctcgggcg ctcgaggacc gggccatcac
     1801 ctcggaggaa gcgaccgagc gctgcctgtc acggatcgcc gagcggaacc cggcgatcaa
     1861 cgccttcatc accgtgctgg ccgacgaggc cacggcacag gcccgcggcg ccgatcgcga
     1921 gatcgcgggc ggccgccgcc ggggcccgct tcacggcgtc ccgatctcgg tgaaggatct
     1981 catcgacctt cggggaacac ccacgacggc ggcctcccgc gtgcgtcagg gccacatcgc
     2041 gcggcaggat gcgaccgtca tcggacggct gcgcacggcg ggagccgtgt tcatcggaaa
     2101 gacgaacctg cacgagttcg ctctgggcac caccaacgaa gactccgcgt atggaccggt
     2161 gcgacatccc ctcgacgaca gccgctcgcc aggtggatct tccggcggtt cggcggcctc
     2221 cgtcctggac ggcatggcgt tcgcgtcggt cggcactgac accggcggat cgattcgaat
     2281 tccgtctgcg gcctgcgggc tggtcgggtt gaagccgacg atcggcgacc tccccaccga
     2341 cggaatcgtg ccgctcagcg cgacgctgga ccacgtcgga ccaatatgcc tgaccgtgga
     2401 ggacaccacg ctgctctacg gcgcgatgcg aggcgtgctc aagcctgagt accccccgct
     2461 gcgcgacgtc cgcggcctgc ggttcggcgt gccgcggccg tacttcttcg atctgctcga
     2521 cactgaggtg gcggcgcggt tcgaggacgc gtgcgggcgc ctgcgatcgg cgggcgcgat
     2581 tctcgaggac gtcctcattc cccataccgc ggagatcgcg acgatttacc tgcacgttgc
     2641 gctgccggag gcggcctcgt gccatgccgc gacgctggag agccagcccg atgactacac
     2701 gacgaacgtg cgtctccggc tggagatggg gcgctatatc ctggccgagg actacgtgcg
     2761 cgcgcggcgc gggcaggaag tgctgacgca ggaggtcaag gacgcgctcg cgggacgcga
     2821 ctgcctgctg ctgccgtcgc ttcccattcc ggccacgcgt cttggcgagc cgatggtccg
     2881 gatcggggag agcgaggaac cggtccggaa cgtcacgctg cgcctgacac aactcttcaa
     2941 catcacgggc catccggcca tcagcgtgcc ctgcggtcgc accaccggcg gcctcccggt
     3001 tggcgcccaa ctcgtgggcg cccgataccg gacggcggat ctgctccagg tcgcggccgc
     3061 ggtcgaacga acgctcgagc attcatagag aaccttgctg aaaaagatct ttcactcgtg
     3121 ggagcgacat ctcgcatcgg tctcgaagga ccgcgtggtc cggcccttcg agtggggcct
     3181 cgactgggtg tcgcacaacg gacacgattt gcacgatccc gccgcgcatc ttggcgaatg
     3241 ggtgcgcgac gtcatgcggg acacgcccgc cttcttcagc gtccagccga cacgagatta
     3301 cgacttcgtg gaagcgaccc ccgcgttgaa acagcagggc gaggcgggca cgctgcgctt
     3361 tccgagcgcg tttgtcacgc cgcatcccga gaacaacgtg gtggtcgggc ggtggtttcc
     3421 gtcggcgagc gagcctcccg tcggcgtcag acgaggccgg ggccgggcgg tcgtggtgct
     3481 gtcgcagtgg aacgccgaca gcgagggcca catcgggctg tcgaagctgc tggcccgctt
     3541 cggcgtgtcc gcgctgcgcc tgagcctccc ctatcacgac acgcgcatgc cgccggagct
     3601 cacgcgcgcc gactacatcg tcagctccaa cgtcgcgcgc acggtccagg tctgccggca
     3661 ggcggtactc gacaccaggc gcgccgtcgc ctggctggcg tcgcagggat tcgatcgcat
     3721 cggcatcctg gggaccagcc tcggctcgtg cctggcgatg ctgacctccg cccacgagcc
     3781 gctgatacgc gcgcaggcgt tgaaccacgt gtccccgtgg tttgccgacg tcgtgtggcg
     3841 ggggttgtcc acccgccacg tgcgaacggg gctcgatggc cacatcgact tggacctgct
     3901 gcgcgcgctc tggctgccga tcagcccgat cgagtacctg gagcgcatcc gtgacaagaa
     3961 gacgctgctc gtctacgcgc gctacgatct gacctttccc gtcgatctct cgcgactgct
     4021 catcgacgag ttcggcaaga ggaacatccc tcacacggcg ttcgccctgc cgtgcgggca
     4081 ctacagcacc ggctgggcgc cgttcaagta cgtggatgga ttcgtgctca cgaagtttct
     4141 gcgcaacagt ctttgacgcc gcgcacgctg aagcgtgcgc tctacgccgt catcgaacac
     4201 tctacgccgt catcgaacac tctacgccgt catcgaacac tcgtagagcg gcggcttcag
     4261 ccgccgcgtg agcgtgaggt tcagagcgtg aggttcagag aaaatcgagc agtccggcca
     4321 gcgcgttgaa gatcacttcc gcgagccgcc ggacgagcgg ggaaatctcg cctccgtcca
     4381 gggcgctggc aacggtcacg ggatcggtga gcaccagcca gatggtggcg ccggcgagca
     4441 cggcggcaat tgcgccgatg agcacggcga cgctcgtcac gcggtcgaag ccgcgctcga
     4501 gatcgtcgtc gcgaatcacc tgttcgagat cctcgctcac ggccgggacc ctcgctagcg
     4561 tagcactcta cttggtcctt agaccctggt ccttgg
//
LOCUS       DBNGSH010000013         5662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1189208, whole genome shotgun sequence.
ACCESSION   DBNGSH010000013 DBNGSH010000000
VERSION     DBNGSH010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1189208"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /gene="larC"
                     /locus_tag="AC16CY_00305"
     CDS             <1..1243
                     /gene="larC"
                     /locus_tag="AC16CY_00305"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR3352242.1"
                     /translation="LAIAPDAVWTERVTRAGISATKFNVRGEALPLDHADARHLGHAD
                     VHDHPHRHDSSAQEHESHSHPHESGSHGHHHESDSRHGDEPHYHVHRTLTEIARLIDA
                     SKLSAAGKSRAKHLFNVLGEAEAEIHGTTLDRVHLHEVGATDSIIDIVGTVFAMEQLG
                     VERVVSSPLNIGSGTIASSHGLYPVPAPATMRLLRGVPVYAGTQAAELVTPTGALLVT
                     GYASEFGPIPAMRVSAIGYGAGTRDFPRSPNVLRVLVGESDAAVSSHSVVVIEAEIDD
                     MNPQIFGVAMDRLMEAGALDVFYTPIQMKKNRPGTLLTVIAPPALKDRLTSVIFRETT
                     TIGVRHRETMRDCLDRETIPVETELGVIRIKVARRGGEPLNAAPEFEDCARLAAEHNL
                     PTKHVQALAMKAYLDRPKRGI"
     gene            1240..3219
                     /gene="metG"
                     /locus_tag="AC16CY_00310"
     CDS             1240..3219
                     /gene="metG"
                     /locus_tag="AC16CY_00310"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR3352243.1"
                     /translation="MSASFITTPIYYINAQPHIGHAYTTMLADAIARSRRLMGGEVFF
                     LTGTDEHGQKVERAAQKAGLATPAFADRVSDSFRQMCRDLRISNDDFIRTTEPRHHAA
                     AQEIWRRVARAGDIYKGEYEGWYCTIDEIFVPEAQLVDGCCPTCGGKVERLKEKSYYF
                     RLSRYQQRLLDFYRDHPDFVQPEFRFNEVRTFVESGLQDLSISRTSFKWGVPVPDDPD
                     HVMYVWFDALTNYLTALGFGSSDPKSEERIGRLWPTVTHFIGKEIVRQHALYWPAFLM
                     SAGLPPPRRIIAHGFWLMGGAKMSKSLGNVARYQDYLDVFGLDALRYFVMREMPLGQD
                     ANFADEGMLTRFNADLANDLGNVVSRAVTMIHRYRGGVVPPLSSSSEAALDRELVAAT
                     ADTIEAVKNEFARAQVSVALQAIWALIGRLNKYIVEREPWTLAKDPAQAGTLDRTLHH
                     SADTLRVVAALIDPVMPETALRIRRMLGVAEERWTSLTAGGLTPGAKLGPVEPLFPRI
                     EKTVEELRNMTQPNETPPAVPAPGAGTAAAQPPPAPPTAPESDGRISIDDFMRVELRV
                     AKVLEAEAVPKSKKLLKLTIDVGSERRTIVAGIAEAYAPDQLVGRTVVIVANLKPAKL
                     MGVESNGMVLAASPDGGLPTLIQVDPAIPAGARVR"
     gene            3313..4104
                     /locus_tag="AC16CY_00315"
     CDS             3313..4104
                     /locus_tag="AC16CY_00315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR3352244.1"
                     /translation="MTVLLIDSHCHIAGPEFAKDLEDVVARARDAGLSGALVVLAADD
                     GEELKQASEVAARWPQVRFTIGVHPHAAGTFAANPDAAAALVKDATASQPLSRAVGEI
                     GLDYHYDFAPRDAQQEVFRHQIRLARLLNRPLVIHTREADDDTIRILTEEGAGDVGGV
                     FHCFTGDRHMARRGLDLGFHLSLAGIVTFPRATELHEVARMVPLDRLLVETDSPFLAP
                     VPHRGTRNEPARVARVAEAIAELRGIAVEAIAEATCTNFERLFTP"
     gene            4179..5183
                     /locus_tag="AC16CY_00320"
     CDS             4179..5183
                     /locus_tag="AC16CY_00320"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3352245.1"
                     /translation="MNPATQNLSAAALAQMFEPIRNDLREVEREFARHVQSQVALIPT
                     IGNYIQNGGGKRIRPAVLLMAARMAGYVGERAVLYASVLEFIHTATLVHDDIIDESEL
                     RRGREAVHTRWGNHVTVLFGDFLYLKSMSLALTQDNLEVIRLLCDVTLRIVEGEIYQL
                     TKNGAVDLTEDEHLDIVRRKTAYLFAGCARIGGMLGPTTREQQEALWDYGLNIGMAFQ
                     IVDDLLDFTGEEVALGKPVGGDLREGKMTLPVIHMRDRGGPRAESVLRKAVDAREVSL
                     EDWHEIRRLMAQTRSIEYARRMASDYVERAKKALCGFPASEAREALLYLPDYVISRDR
                     "
     gene            5297..>5662
                     /locus_tag="AC16CY_00325"
     CDS             5297..>5662
                     /locus_tag="AC16CY_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR3352246.1"
                     /translation="MQELQRLEADQPELVTPDSPTQRVSGRVAEGFRTVAHAEPMLSL
                     DNAYTEEELRAFDDRVRRGLGAGGSVPDAVDYVAELKIDGLSIALTYVDGLLERAATR
                     GDGTRGEEVTSNVRTIRAIP"
BASE COUNT          996 a         1867 c         1887 g          912 t
ORIGIN      
        1 cctggcgatc gctcccgatg cggtctggac tgagcgcgtg acacgcgccg gcatcagcgc
       61 gacgaagttc aacgttcgcg gcgaggcgct cccgctggac catgccgacg cccgtcacct
      121 ggggcacgcc gacgtgcacg accacccgca ccgacacgac tcgagtgcgc aggaacacga
      181 atcacattcc cacccgcatg agtcaggctc gcacgggcac caccatgaat cagactcgcg
      241 ccatggtgat gaaccccact accacgtcca ccgcacgctg accgagatcg ccaggctcat
      301 cgacgcgtcc aagctgagcg cggcaggaaa gagtcgagcc aagcacctgt tcaacgtgct
      361 tggggaagcc gaagccgaga ttcacggcac gacgctcgac cgggtccacc tgcacgaggt
      421 gggcgcgacc gactccatca tcgacatcgt cggcaccgtc tttgcgatgg agcagctcgg
      481 cgtcgagcgg gtcgtgtcgt cgccgttgaa tatcggcagc ggcaccatcg cgtcttcgca
      541 cggtttgtat ccggttcccg cgcccgccac gatgcgcctc ctgcgcggcg tgcccgtgta
      601 tgcaggaacc caggctgccg aactggtgac gccgaccggc gccctgctcg tcaccggcta
      661 cgccagcgag ttcggcccca ttccggccat gcgcgtgtca gcgatcggct acggcgccgg
      721 gacccgggac ttcccgcggt cacccaacgt cctgcgcgtc ctggtcggcg aatcggacgc
      781 agccgtctcg agccactcgg tcgtcgtgat cgaggccgag atcgacgaca tgaatccgca
      841 gatcttcggc gtcgcgatgg atcgcctgat ggaggcgggc gcgctcgacg tgttctacac
      901 gccgatccag atgaagaaga accgacccgg gacgctgctg accgtgatcg ccccgccggc
      961 gctcaaggat cgtctgacat cggtcatctt ccgggaaacg acgacgatcg gcgtccgcca
     1021 ccgcgagacg atgcgtgact gcctcgatcg ggagacgatt ccggttgaga ccgagctggg
     1081 cgtgatcagg atcaaggttg cccgcagagg aggggagccg ctgaacgccg cgccggagtt
     1141 cgaggattgc gcccggctgg cggccgagca caacctgccg accaagcacg tccaggcgct
     1201 cgcgatgaag gcttatctgg atcgcccgaa gcgggggata tgagcgcgtc cttcatcacc
     1261 acaccgatct attacatcaa cgcccaacct catatcggcc acgcatacac gacgatgctg
     1321 gccgatgcca tcgcgcgctc gcgtcgcctc atgggcggcg aggtgttctt cctgaccggt
     1381 accgacgaac acggacagaa agtcgagcgc gcggcgcaga aggctggcct ggccacgccg
     1441 gccttcgcgg accgcgtgtc cgactcgttc cggcagatgt gccgcgacct gaggatctcc
     1501 aacgacgact tcatccggac gacggagccg cgccatcacg cggcggcgca ggagatctgg
     1561 aggcgcgtcg cccgggcggg cgacatctac aagggcgagt acgaaggctg gtattgcacg
     1621 atcgacgaga tcttcgtgcc cgaggcgcag ctggtcgatg gctgctgccc gacctgcggc
     1681 ggcaaggtcg agaggctcaa ggaaaagagc tactacttcc ggttgtcccg ctaccagcag
     1741 cggctgctcg acttctaccg ggaccatccg gacttcgtgc agcccgagtt caggttcaac
     1801 gaggtccgca ccttcgtcga gtcggggctc caggatctga gcatcagccg gacgtccttc
     1861 aagtggggcg ttccagtacc cgacgatccg gatcacgtga tgtacgtgtg gttcgatgcg
     1921 ctcacgaact acctgacggc gctcggcttc ggctcgagcg atccgaaatc ggaagagcgc
     1981 atcggccggc tgtggccgac ggtcacccac ttcatcggca aggagatcgt ccggcagcac
     2041 gcgctgtact ggccggcgtt tctgatgtcg gcgggtcttc cgcctccgcg acggatcatc
     2101 gcgcacggtt tctggctgat ggggggcgcc aagatgtcga agtcgctcgg caacgtggcg
     2161 cggtaccagg actacctgga cgtgttcggg ctcgacgcgc ttcgctactt cgtgatgcgc
     2221 gagatgccgc tcggccagga tgcgaacttc gcggacgagg ggatgctcac gcgcttcaac
     2281 gccgacctgg ccaacgatct cggaaacgtc gtgagccggg ccgtcacgat gatccatcgc
     2341 tatcgcggcg gcgtcgtgcc gcctctgtcc agctcgagcg aggccgcact ggatcgcgag
     2401 ctcgtggccg cgacggcgga cacgatcgag gcggtgaaga acgagttcgc tcgcgctcag
     2461 gtcagcgtcg cgctccaggc catctgggcc ttgatcggcc gcctgaacaa gtacatcgtc
     2521 gagcgcgagc cctggacgct tgcgaaagat ccggcgcagg ccggcacgct cgaccggacg
     2581 ctccatcact cggcggacac gctgcgcgtc gtcgccgcgc tgatcgatcc ggtcatgccg
     2641 gaaacagcgc tcaggatacg gaggatgctg ggcgtcgctg aagaacggtg gacgtcgctg
     2701 accgcggggg ggctcacgcc gggcgcgaag ctgggtcccg tggaaccact gttcccgcga
     2761 atcgagaaga ctgtcgagga gctgcgaaac atgacacaac ccaacgaaac gccgccggcg
     2821 gtgccggccc cgggcgcagg aaccgccgca gcgcagccgc cgccggcccc gccgacggcg
     2881 ccggagagcg atgggcgaat cagcatcgac gacttcatga gggtcgagct gcgtgttgcg
     2941 aaggtcctcg aggcagaggc cgtgccgaag tcgaagaagc tgttgaagct cacgatcgac
     3001 gtcggttccg agcgccggac gatcgtcgcc ggcatcgcag aggcgtacgc tcccgatcag
     3061 ctcgtgggcc ggacggtcgt gatcgtggcc aacctcaagc cggcgaagct gatgggagtc
     3121 gaatccaacg gcatggtgct cgccgccagt cccgacggcg ggttgccgac gctgattcag
     3181 gtcgatccgg ccatccccgc cggggcgcgc gtgcgttgag cggggaacgc gcgcgagtgt
     3241 gtcaacttcc aattcccaac gcccaactcc caatcgcggg tttcctggga gttgggagtt
     3301 ggaggttggg agttgacggt cttgctgata gatagccact gccacatcgc cggcccggag
     3361 ttcgccaagg atctcgagga tgtcgtggcc cgggcgcgtg acgcgggtct gtcgggcgcg
     3421 ctcgtcgtgc tggccgccga cgacggcgaa gaattgaaac aggcgtcgga ggtcgcggcg
     3481 cggtggccgc aggtccggtt cacgatcggc gtgcatccgc acgcggccgg cacgttcgcg
     3541 gcgaacccgg acgccgccgc cgccctggtg aaggacgcga ccgcgtcaca gcctttgtcg
     3601 cgcgccgtgg gcgagatcgg tctggactac cattacgact tcgcgccgcg cgacgcacag
     3661 caggaggtgt ttcgtcatca gatcaggctg gcgcgcctgc tgaatcgccc cctcgtcatc
     3721 cacacgcgcg aagccgacga cgatacgatc cggatcctca ccgaagaagg ggcaggcgat
     3781 gtcggtggtg tctttcactg cttcaccggc gaccggcaca tggcccggcg cgggctcgac
     3841 ctcggattcc acctctcgct cgccggcatc gtgacgtttc ctcgagccac ggaactgcac
     3901 gaggtggccc gcatggtgcc gctcgatcgc ctgctcgtcg agaccgacag cccctttctc
     3961 gcgccggtcc cgcaccgggg cactcgcaac gagccggccc gcgtcgcccg tgtcgccgag
     4021 gcgatcgccg aacttcgcgg aatcgccgtc gaggcgattg cggaggcgac ttgcacgaat
     4081 ttcgagcgcc tgttcacgcc gtaactccaa gcgatcacac ggattaggcc gccacgcgcg
     4141 ttgacactcc atacggcgtt atgctctgat ctgacaactt gaatcccgcc acgcagaatc
     4201 tttccgccgc tgctctcgcg cagatgttcg agcccattcg caacgacctc cgcgaggtgg
     4261 agcgcgagtt tgcccgccac gtgcagtccc aggttgcgct gattcccacg atcggcaact
     4321 acatccagaa cggcggcggc aagcgtattc ggcccgccgt gctgctcatg gcggcgcgca
     4381 tggcggggta cgtgggcgag cgggccgtgc tctacgcctc ggttctcgag ttcatccaca
     4441 cggcgacgct ggtgcacgac gacatcatcg acgagtccga gctgcgccgc ggccgtgagg
     4501 cggtgcacac ccgatggggc aaccacgtga cggtgctctt cggggacttc ctgtatttga
     4561 agtcgatgtc gctcgcgctc acccaggaca atctggaggt catccggctt ctgtgcgacg
     4621 tcacgctccg tatcgtcgag ggcgagattt atcagctgac caagaacggc gcggttgacc
     4681 tcaccgaaga cgagcatctg gacatcgtgc ggcgcaagac cgcctatctg ttcgcgggct
     4741 gcgcccggat cggcggcatg ctgggcccga cgacgcgcga gcagcaggag gcgctgtggg
     4801 actacggcct caacatcggc atggcgtttc agatcgttga cgacctgctc gacttcaccg
     4861 gcgaggaagt ggccctcggt aagcccgtcg gcggcgacct gcgtgaaggg aagatgacgc
     4921 ttcccgtgat ccacatgcgc gaccggggcg ggccgcgggc ggagagcgtc ctgaggaagg
     4981 ccgtcgatgc acgcgaggtg agcctcgaag actggcacga gattcgccgc ctgatggcgc
     5041 agacgcgctc catcgagtac gcccggcgaa tggcatccga ctacgtcgag cgcgccaaga
     5101 aggcgctctg cggcttcccc gcgtcggagg cacgagaggc gctgctgtac ctccctgact
     5161 acgtcatttc acgggaccgc taatcctgga cccggtttcc cgaatcgcag aactccgccg
     5221 cctgattcgc caccacgaag agcgctatta cgtcgccaac cagccggaga tcagcgacgc
     5281 cgagttcgac gggctgatgc aggagctgca gcggctcgag gccgaccagc ccgagctcgt
     5341 cactccggat tcaccgacgc agcgggtgag cggacgtgtg gccgaagggt tcaggacggt
     5401 tgcgcacgcc gagccgatgc tgagcctcga caacgcgtat accgaggagg aactgcgcgc
     5461 ctttgacgat cgggtgcgcc gcgggctggg tgcgggcggg agcgtgccag acgcagtgga
     5521 ctacgtcgcg gagctcaaga tcgatggtct cagcatcgcg ctgacttacg tggacgggtt
     5581 gctcgagcgc gcggccacgc gcggagacgg cacacgcggc gaggaggtga cgtcgaacgt
     5641 ccgcacgatt cgagccattc cg
//
LOCUS       DBNGSH010000014         5568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_170537, whole genome shotgun sequence.
ACCESSION   DBNGSH010000014 DBNGSH010000000
VERSION     DBNGSH010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_170537"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="AC16CY_00330"
     CDS             <1..668
                     /locus_tag="AC16CY_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HIR3352247.1"
                     /translation="RDCVQKTLDLQPDSVTIYQMELPFNTTISGNLLKGTGRFAHPVA
                     DWQTKRRWVREAFEALEAAGYHVRSAYTAVKDPSRTRFVYVDRLWQGADMIGLGVASF
                     GHVNGVHIQNLDTWEAYSDAIRRNEIPLSRAYRPTPDERLIREFILQLKLGSIAPGYF
                     KDKYRVDVRDRFRDGLESLQAEGYLSACTAEKVALTREGLLRVDGLIKRFFLPQHVGI
                     RYT"
     gene            714..1142
                     /locus_tag="AC16CY_00335"
     CDS             714..1142
                     /locus_tag="AC16CY_00335"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3352248.1"
                     /translation="MPRRSEYTLRRRVQFYEADSAGIVHFSNYFRYMEEAEHALWREA
                     GVNIAPRGSDVGFPRVAASFEYLRPLYFGDEFEARIRVAAMGEKSIRYTCTLKKGEEK
                     IGTGSVTVVCVGKREGGGMKARPFPPEIADRFEVSAEDRE"
     gene            1143..2432
                     /locus_tag="AC16CY_00340"
     CDS             1143..2432
                     /locus_tag="AC16CY_00340"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR3352249.1"
                     /translation="MQAVEQLGRDQLTSHQGRRLGELLAALHGRNAFYTRKLDEAGVD
                     PGRMRFPEDLKSLPLTTKAELQADQDANPPWGTALTEPLVRYTRYCQTSSSTGRPLRW
                     IDTNESWQWLLDCWKAVFRGARVVPGDRVFFPFSFGPFLGFWAGFEAGPQLGLHCVPG
                     GGMSTQIRLSMIEAIQATVVCCTPTYALRLAEINELEHRPPLAKGSVRTLIVAGEPGG
                     SIPATRERIERSWGARVIDHHGMTEVGPISFECWESPGGLHVNEGEFIAEVLDPVTGE
                     DVADGQMGELVVTNLGRTASPVIRYRTRDLVVRRSEPCRCGRNWARLEGGILARADDM
                     VNIRGVNVYPVGIEAVLRKLPEIVEFRSIVSRPGAMRSLRVEIEPAPGVDDHAALASK
                     VSYQLREAIGLSVFVHVVDRGVLPRFEMKAGRFVVEA"
     gene            2432..3742
                     /locus_tag="AC16CY_00345"
     CDS             2432..3742
                     /locus_tag="AC16CY_00345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3352250.1"
                     /translation="MSAVSPDVVVIGGGPAGSTVSTLLAKEGVAVQLFERERFPRFHI
                     GESLIPETYWVLKQLDMLPKMQASRHQKKFSVQFVNSEGKESAPFYFWDNKPHECSQT
                     WQVVRSEFDQLMLENAREHGVDVHEGVRVHDVLFENDRAVGVRIKDESGLRDVRAKVV
                     VDASGQNGLLMNRLGLRLWDPILNKGAIWTYWEGAYRDTGRDEGATLVVQNGNKQGWF
                     WYIPLHSNIVSVGVVAPFDYLFKGRTNYSQTYEEEVNLCPGVKHRVAGAKQVTGHFAT
                     KDYSYRSTKVAGDGWVLVGDAFGFLDPLYSSGVLLALKSGQLASQAIVEGLARGDTSG
                     AQLGKWGPVFNEGVDRMRRLVCEYYSGFSFGKMIREYPELRGTLTDLLIGDLFHDGTD
                     KVWGPLESLYEEGKAPIPSWNAGTPPATAADKANLLVLPEGRRP"
     gene            3751..5079
                     /locus_tag="AC16CY_00350"
     CDS             3751..5079
                     /locus_tag="AC16CY_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3352251.1"
                     /translation="MSRVMMALVALAIGVAQATLVGENWPQFRGTKAGVGVDHPDLPD
                     TWSTKDNVAWVAEIPGLGWSSPVVWGDHVFLTTVVNSGQQEAPKPGFYIGDWPASSAP
                     HRWMVYDIDFATGKVRWSRELGGAPPGRPKHLKNSYASETPVTDGERVYVYFGNLGLF
                     ALDFSGKPLWSKTMGPFKTRNNWGTGASPVLHRGRVYIVNDNDDGSFLAAYDARTGDQ
                     LWRVDRQEGTNWATPFIWENEMRTEIVTSGSDLVRSYDLSGKLLWQLKGMSSISIPTP
                     FDRFGLLYISSGYVPDPLRPTYAIRPGASGDVSLKTGEATNSHIVWSLPTGAPYNPTP
                     IVYGDVLYTLFDRALFTSHDAKTGKEIYGRQRITADAAQFTASPWAYNGKVFALSEEG
                     DTYVVQAGPTFNVVAKNSLNEMALATPAVARGSLFVRTQSKLYRITKSRK"
     gene            complement(5100..5429)
                     /locus_tag="AC16CY_00355"
     CDS             complement(5100..5429)
                     /locus_tag="AC16CY_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3352252.1"
                     /translation="MTFGSDTIFIGIRGTVLALDRGTGDTRWSKHLVSSDFVNVTFQE
                     GDLYAATKGRLYRLDPATGEILWSNELPGLGWGIITIAGAPQAAPSAEKRRRDQQAAA
                     AGAAASS"
     gene            complement(5431..>5568)
                     /locus_tag="AC16CY_00360"
     CDS             complement(5431..>5568)
                     /locus_tag="AC16CY_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitropropane dioxygenase"
                     /protein_id="HIR3352253.1"
                     /translation="AVAPLRAKAESQGSGDFSPLWSGQNASGCKEIPAAKLTRELAGG
                     N"
BASE COUNT          967 a         1755 c         1914 g          932 t
ORIGIN      
        1 ggcgcgactg cgtgcagaag acgctcgacc tgcagcccga cagcgtgacg atctaccaga
       61 tggagctgcc gttcaacacg acgatcagcg ggaacctgct gaagggcacg gggcggttcg
      121 cgcaccctgt cgccgactgg cagaccaagc gccgctgggt gcgcgaggcg ttcgaggcgc
      181 tcgaagcagc cggctaccat gtgcgaagcg cctacacggc cgtcaaggat ccctcgcgga
      241 cgcgcttcgt gtacgtggac cggctgtggc agggcgccga catgatcggg cttggcgtcg
      301 cgtcgttcgg gcatgtcaac ggcgtgcaca ttcagaacct cgatacgtgg gaggcctact
      361 ccgacgcgat tcgccggaac gagattccgc tgagccgggc ctaccggccg acgccagacg
      421 aacgtctcat ccgcgagttc atcctgcagc tcaagctcgg ttcgattgcg ccgggctact
      481 tcaaggacaa gtaccgcgtg gatgtccgcg atcgcttccg cgacggcctc gagtcgcttc
      541 aggccgaagg ctacctctcg gcgtgcacgg cggagaaggt cgcgttgacg cgagagggcc
      601 tgctgcgcgt tgatggcctg atcaagcgct tcttcctgcc ccagcacgtc gggattcgct
      661 acacatgatc gcggcaccct cgcaccttcg caccctcgca ccctcgcacc gttatgcctc
      721 gccgttctga gtacaccctt cggcgccgcg tgcagttcta cgaggcggac tcggccggca
      781 tcgtgcactt cagcaattac ttccgctaca tggaagaggc cgagcacgcg ctctggcgcg
      841 aggcgggcgt caacatcgcg ccaagggggt cggacgtcgg tttcccccgc gtcgcggcgt
      901 cgttcgagta cctgcggccg ctctatttcg gggacgagtt cgaggcgcgc atccgcgtcg
      961 ccgccatggg cgagaagagc atccggtata cgtgcacgct gaagaagggt gaggagaaga
     1021 tcggcacggg aagcgtgacg gtcgtgtgcg tgggcaaacg ggagggcggc ggcatgaagg
     1081 ccaggccgtt cccgccggag atcgcggacc gcttcgaggt ctcggccgag gaccgcgagt
     1141 aaatgcaggc ggtcgagcag ctgggaaggg atcaactgac gtcgcatcag ggccggcggc
     1201 tcggggagct gctggcggcc ctccacggcc ggaacgcgtt ctacacgcga aagctggacg
     1261 aggcaggcgt cgatcccggt cgaatgcggt ttcccgagga cctgaaatcg ctgccgctga
     1321 cgacgaaggc cgagctgcag gccgatcagg acgcgaaccc gccgtggggc accgcgttga
     1381 ccgagccgct cgtccgctac acgcggtact gccagacgtc ctcgtccacc ggccggccgc
     1441 tgcgttggat cgatacgaac gagagctggc aatggctgtt ggactgctgg aaggcggtgt
     1501 tccgcggcgc gcgcgtcgtg cccggagacc gcgtgttctt tccgttctcg ttcggcccgt
     1561 tcctgggctt ctgggccggc ttcgaggctg gtccgcagct tggcctgcac tgcgtgcccg
     1621 gcggcgggat gtcgacgcag attcgcctgt cgatgatcga ggccatccag gcgaccgtgg
     1681 tgtgctgcac cccgacctac gcgctgagac tggcggagat caacgagctg gagcaccggc
     1741 cgccgctggc gaaggggagc gtgcgcacgc tgatcgtggc cggcgagccc ggcggcagca
     1801 ttcccgcgac gcgtgagcgg atcgagcgga gctggggcgc gcgagtgatc gaccaccacg
     1861 gcatgacgga ggtcggtccc atcagcttcg agtgctggga gtcgccgggc ggactgcacg
     1921 tcaacgaggg agagttcatc gcggaagtgc tcgacccggt gaccggcgaa gacgtggccg
     1981 acggccagat gggagaactg gtcgtgacga acctcgggcg gacggcaagc cccgtcattc
     2041 gctaccggac gcgggacctc gtcgtccggc gctccgagcc gtgccgctgc gggcgaaact
     2101 gggcgcggct cgagggagga atcctggcca gggcggacga catggtgaac atcagaggcg
     2161 tcaacgttta ccctgtgggt atcgaggccg tcctgcggaa gctgcccgag atcgtggagt
     2221 tcaggtcgat cgtgtcgcgc cctggagcga tgaggtcgct gcgcgtcgag atcgagcccg
     2281 cgccgggggt ggacgatcac gccgccctcg cctcgaaggt gtcgtatcag ttgcgcgagg
     2341 ccatcgggct gagcgtgttc gtccacgtgg tcgatcgggg cgtgctgccg cgcttcgaga
     2401 tgaaggcggg ccggttcgtc gtggaggcat agtgagcgca gtcagtccgg atgtcgtcgt
     2461 gatcggcggt gggccagcgg gatccaccgt gtccacgctc ctcgcgaagg agggcgttgc
     2521 cgtccagctc ttcgagcgcg agcgctttcc gcggtttcac atcggcgagt cgctcatccc
     2581 ggagacctac tgggtgttga agcagctcga catgctgccg aagatgcagg cgagccggca
     2641 ccagaagaag ttcagcgtgc agttcgtcaa ctccgaaggg aaggaatcgg ccccgttcta
     2701 cttctgggac aacaagccgc acgagtgctc ccagacgtgg caggtcgttc gcagcgagtt
     2761 cgaccaactg atgctggaga acgcccgcga gcacggcgtg gacgtgcatg aaggcgtgcg
     2821 ggtgcacgac gtcctgttcg agaacgaccg ggctgtcggc gtcaggatca aggacgagag
     2881 cggacttcga gacgtccggg cgaaggtcgt cgtcgatgcg agcggacaga acgggctgct
     2941 gatgaaccgg ctggggctcc ggctctggga tccgatcctg aacaagggcg ctatctggac
     3001 ctactgggaa ggggcgtacc gcgacaccgg ccgggacgag ggggcgacgc tcgtcgtcca
     3061 gaacgggaac aagcaaggct ggttctggta catcccgctc cacagcaaca tcgtcagcgt
     3121 gggcgtggtg gcgccgttcg attacctctt caagggccgc acgaactact cgcagactta
     3181 cgaggaagag gtcaatctct gtccaggcgt caagcaccgc gtcgccggcg caaagcaggt
     3241 caccggccac ttcgccacga aagactactc gtacaggtcc accaaggtgg ccggagatgg
     3301 ctgggtcctg gtgggggacg cgttcggatt cctcgatccg ctgtattcat ccggcgtcct
     3361 gctggcgctc aagtccggcc agcttgcctc gcaggccatc gtcgagggat tggctcgagg
     3421 cgacacctca ggagcgcaac tcggcaagtg gggtccggtc ttcaacgaag gcgttgatcg
     3481 catgaggcgc ttggtgtgcg agtactacag cgggttcagc tttggaaaga tgatccgcga
     3541 gtacccggag cttcgcggaa cgctgacgga cctgctcatc ggggacctgt tccacgacgg
     3601 caccgacaag gtgtgggggc cgctcgagtc gctgtacgag gaaggaaagg ccccgattcc
     3661 gtcatggaac gcgggcacgc cgccggcgac cgccgcggac aaggcgaacc tgttagtgct
     3721 gccggaaggg cggcgaccgt aggagggtac atgagtcgcg tgatgatggc gctcgtggcg
     3781 ctcgccattg gtgtggctca ggccacgctc gtcggcgaga actggccgca gttcagaggc
     3841 acgaaggcgg gtgtcggcgt cgatcacccg gatctcccgg acacgtggag cacgaaggac
     3901 aacgtggcgt gggtcgcgga gatcccggga ctcggctgga gctcgcccgt cgtgtggggc
     3961 gaccacgtgt tcctgacgac ggtcgtcaac tctgggcagc aggaggcgcc gaagccgggc
     4021 ttctacatcg gcgattggcc cgcctcgagc gcccctcacc gctggatggt gtacgacatc
     4081 gactttgcga cgggtaaggt gcgctggtcg cgcgagctgg gcggcgcgcc gcctggacgc
     4141 cccaagcacc tcaagaacag ctacgcctcg gaaacgcccg tgaccgacgg cgagcgcgtg
     4201 tacgtctact ttggcaacct cgggctcttt gcgctggact tctcgggcaa gccgctctgg
     4261 tcgaagacga tgggtccgtt caagacgcgc aacaactggg gcacaggagc ctcgccagtc
     4321 ctccaccgcg gccgcgtgta cattgtcaac gacaacgacg acgggtcgtt cctggcagcg
     4381 tacgacgcgc gaacgggcga tcagttgtgg cgcgtggacc ggcaggaggg gacgaactgg
     4441 gccactccct tcatctggga aaacgagatg cggaccgaga tcgtgacctc aggatccgac
     4501 ctggtccgat cctacgactt gtcgggaaag ctgctgtggc agctgaaggg gatgtcgtcg
     4561 atctccattc cgacgccgtt cgatcgcttc gggctgctct acatcagctc ggggtacgtc
     4621 cccgacccgc tgcggccgac ctacgccatc cgcccgggcg cctccggcga cgtctcgctc
     4681 aagacgggcg aggccacgaa cagccatatc gtctggtcct tgccgaccgg cgcgccttac
     4741 aacccgaccc cgatcgtcta cggcgacgtg ctctacacgc tcttcgatcg cgcactgttt
     4801 acgagtcacg acgcgaagac cggcaaggag atctacgggc ggcagcgtat aaccgccgat
     4861 gctgcccagt tcaccgcctc cccgtgggcg tacaacggga aggtcttcgc cctcagcgag
     4921 gagggcgata cctacgtcgt ccaggcaggt ccgacgttca acgtggtcgc gaaaaactca
     4981 ctgaacgaga tggccctggc aacgccggcc gtggcacgcg gcagcctgtt cgtacggacg
     5041 caatcgaagc tctaccggat tacgaaatcc cggaagtagc ggccggcacg gggatttgct
     5101 cacgacgagg cggcggcacc agcagcagct gcctgctggt cacggcgcct tttctccgcc
     5161 gacggcgctg cctgcggcgc cccggcaatc gtgatgatgc cccatccgag tcccggaagc
     5221 tcgttgctcc agaggatctc gcccgtcgcc gggtcgagac ggtagagccg tcctttcgtg
     5281 gccgcgtaca ggtccccctc ctgaaaggtg acgttcacga agtcggagct caccagatgc
     5341 ttgctccacc gcgtgtcgcc ggtccctcgg tcgagggcga gcaccgtccc cctgatgccg
     5401 atgaagatcg tgtccgaccc aaatgtcatc tcagttgcct ccagccagtt ctctcgtcaa
     5461 cttcgctgcc gggatctcct tgcacccact tgcattctgg ccggaccaga gcggcgagaa
     5521 gtcgcccgat ccctggctct cggctttcgc gcgcaacgga gcgacggc
//
LOCUS       DBNGSH010000015         8857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3906329, whole genome shotgun sequence.
ACCESSION   DBNGSH010000015 DBNGSH010000000
VERSION     DBNGSH010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3906329"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="AC16CY_00365"
     CDS             complement(<1..368)
                     /locus_tag="AC16CY_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352254.1"
                     /translation="MRYFASPGVAIGFSAFMLCAETCLHAESFLAADATAFELPIHDW
                     AAAVFLLTAGVLSHRSWTLTRRQYQAVAWGFMLSLLVGAFVPSVQEWLTPPNIVDWGI
                     SEGAFVGIILVLIVIALCGLV"
     gene            complement(386..1603)
                     /locus_tag="AC16CY_00370"
     CDS             complement(386..1603)
                     /locus_tag="AC16CY_00370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3352255.1"
                     /translation="MTRRQLLETATMSAAAAAFARTPASAQAPLGAANEKVRAVLESI
                     VKDTTEIGLQVAAYVGGKLVIDAWAGMADEATKRPVDGDTMFMLSSTTKGVTATCMHV
                     MAEKHKISYDMPIVKVWPEFGQNGKEAATLRHALSHQTGVPQTPVGYTPDWLPDWDRM
                     CRGIAALKPMYPIGQRTAYHSLNYGYINGEILRRVDGRTIAQFLQDEICKPLGINGIY
                     LGVPDRELQRVAVLTDAPPAPAEYDARMVGEPAGSYVARVFNRREVQQASIPGSGGIM
                     SARGLARHYAMLANWGELDGVRVLPEARVRAGIELQSYEWDEVYWIRARRALGYRRGR
                     DTGPLASEEAFGHVGGGGSFGYADPARRLGIGFTKNYFNYATGGAVNRGQPPRAASNV
                     VTEAVFDALGLKR"
     gene            1672..2997
                     /locus_tag="AC16CY_00375"
     CDS             1672..2997
                     /locus_tag="AC16CY_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR3352256.1"
                     /translation="MHTGQPAPAAHSPLLTRTWYSYYVLGALTICYVINTMDRSQILA
                     ASLQSIKKEFAASDFQMGLLTGVPFAIFYSVMGIPIAAWADRTSRRNVLALAVASWSA
                     MTAIFGMSVNYAMLFAARVGTAIGEAGGSPPSHSLISDYFPKSRRGTAFAIFALGVPI
                     GTSLGAAYGGWGNQNFGWRNTFILAGVPGVVIALFVWLTVKEPPRGLSDGVARKTDSK
                     VPGMLEVMSFLWRRSSFRHLCLAAALHSVVWYASSAFNNAFLQRSHQLTVAEASYWIA
                     VLAAFAGGGTFLGGWLSDRLSARSNDRRWYMWVPGVATLLCVPFQFLAFLSTSLAVAL
                     PSFVGLMVMAAVFFGPSFAMTQALATLRMRSVATSLLLFIQTLIGFGLGPSVTGYISD
                     RLAPTYQVDSLRYALVIIGVVNLWAAVHYMLGARTLKQDLETTEKLATA"
     gene            3216..4148
                     /locus_tag="AC16CY_00380"
     CDS             3216..4148
                     /locus_tag="AC16CY_00380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3352257.1"
                     /translation="MSDQYDVVIVGGGVAGLSAGLTSARAARKTRVLTGPSIGGNLLS
                     IERIDGYPGFPDGVPGYDLCPIIQEQAVAAGAEFTAAEATGVQASEGGWRLTTSGGDV
                     WARAVVVATGSALKTLGIPGEERLTGKGVSHCASCDAPLLRGRTVGVVGGGDSALQEA
                     LTLAQHAARVIVLHRGSAFSAQEAYRQRVLENPKIEVRFGTIVEEAVGNGGLTGVRTR
                     SAGDGKTGDLELAGLFVYVGLAPVTTWLGSALALDASGRVPVDGAMRSTLPGLFAAGT
                     VRSGSAGRAAAAAGDGATAAVSADQYVKDGKWRA"
     gene            4183..5304
                     /locus_tag="AC16CY_00385"
     CDS             4183..5304
                     /locus_tag="AC16CY_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HIR3352258.1"
                     /translation="MRTLSGAAAIAGLLVVGALAQAPEKGGGDVTGSYELVAGWPQNW
                     CGEGFVIGSTAGIWAESPDRVIVFARGCLPRLENAGDVVPTRNASGFDLSQKDPSRHP
                     RWDHIVNIVDRNGRLLESWEQHNKLFVRPHRVLVSPYDAERHVWLVDDGAHAIYKFTR
                     DGKQLVMTVGTPRMPGNDETHFARPTDVAFLPDGTFFVSDGYTNTRVVKFDRNGKYVM
                     TWGMRGTPPNETRPYYMNTVHAVVTDKQRRVYVSDRANHRIQIFDENGKFLDAWPNVP
                     LPYSLLMTDDQFLWSASGQTQKFTKYDLNGRLLAAWGTFGAFPGGFWGVHQFHVDSEG
                     SLYTADVHVGRPQKFRPRPGADRTRLIGAPMRVGVTSTR"
     gene            5364..6989
                     /locus_tag="AC16CY_00390"
     CDS             5364..6989
                     /locus_tag="AC16CY_00390"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5652..5654,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR3352259.1"
                     /translation="MTQRITVHDPRGYPPKVVGKRLASRLPTLDGKTVYLVDCLFDNS
                     AVFMDQLQQWFGEHLPSVDARIIRPKETWADDPEMRAAIAKDGDAAILGIGLUSTCSP
                     TVVGLTAALEEGGVPSVAVHTQRFARLCKATALSTGMPRLRQAYTPHPVVDQPAERLR
                     AYIDGTDAISGRPFMQEVLDGLTTPLEDEDLQGLSFERTTPRLVEPAAEDDLQRLFVE
                     NHWTDMLPIVLPTEARVEAMLKGTSHPPDKIVGRLRPTAFRESWEFDVEKVAVNAVMA
                     GARPAYFPVILALAASGITARSSSTTSFATISVINGPIRNEIAMNSGIGVMGPYNHAN
                     ATIGRAYGLLSQNLQGGSVPNETYMGSHGNWYAYTAVIPEAEERSPWQPLHVQKGFKP
                     AESVAFVFFGGWYTHAGYGPRATWKEKMRHALAAVEQYSPPLFVMDPIVARGFVDLGF
                     DTKEKLIDWCAENALLTAREYWDNQSIQLLRPKAVAGIEPYASRLKAPPDEVIKIYEP
                     SDIHIVVAGGETQGAWKMISGALRGTVSIDAWR"
     gene            6986..8605
                     /locus_tag="AC16CY_00395"
     CDS             6986..8605
                     /locus_tag="AC16CY_00395"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7265..7267,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR3352260.1"
                     /translation="MITVHDPRGYPPKIVGKRLAPRLSTLDGKTVYLVDCLFDNSDVF
                     MEQLRQWFAERLPSVKTRIARPKETWSDDEQMRAAIRADGHAAILGVGLUSTCSPTVV
                     GLAAALEQDGVPSIAVHTHVFARLCKATALSMGMSNLRQAYVPQPVVDRSPGDLRAYI
                     EGHDPVSKRPFMQEVLDGLTVPPDDADLQGLSFERTTPRLVEPDTEDNLRRLFIDNNW
                     TDSLPIVLPTEERVEAMLRGTSHPPDKVVGRLRPTAFREIWEFTVEKVAVNAVMAGAR
                     PEYFPVILALASSGISARSSSTNSFATISVVNGPIRNEIGMNSGIGVMGPYNHANSTI
                     GRAYGLLSQNLQGGSIPGDTYMGSLGNWLAYTAVLPEAEERSPWQPLHVQKGYKPTDS
                     VASIFFGGWYTHAGYGPRATWQEKMRRSLAAVEQYSQPLLVVDPIVARGFVDLGFDTK
                     EKLIDWCAQNALMTAREYWDNQWIQYLMRPRAVAGVEPYASRLKADPDEMIRIFEPSD
                     IHIVVAGGETQGAWKLLSGSLRATVSIDAWR"
     gene            complement(8663..>8857)
                     /locus_tag="AC16CY_00400"
     CDS             complement(8663..>8857)
                     /locus_tag="AC16CY_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352261.1"
                     /translation="LEKDDAAAAAARAKAAAETPAGPSEAAASTPIRAPKHQTQQPWK
                     ATATRGFAFRPRTPRKVGGS"
BASE COUNT         1459 a         2925 c         2997 g         1476 t
ORIGIN      
        1 acgagcccgc acagcgcgat gacgatgaga acgaggatga tcccgacgaa cgcgccctcc
       61 gagatgcccc aatcgacgat gttgggaggc gtcagccact cctgcaccga cggcacgaac
      121 gcgccgacca gcaggctgag catgaagccc cacgccacag cctggtattg gcggcgggtg
      181 agcgtccacg accgatggct caggacaccg gcggtcagca ggaacacggc cgcggcccag
      241 tcgtgaatcg gcagctcgaa cgccgtcgca tcagcggcca gaaagctctc ggcatggaga
      301 caggtttcgg cgcacaacat gaacgcgctg aaaccgatgg cgacaccggg gctggcgaag
      361 tacctcataa gatggtctcc cttcctcaac gcttcaggcc cagtgcgtcg aagacggcct
      421 cggtgaccac gttcgacgcc gcacgcggcg gctggccgcg attcactgcc ccgccggtcg
      481 cgtagttgaa gtagtttttc gtgaatccga tgccgagtcg cctggcagga tcggcgtaac
      541 cgaacgagcc gccgccgccg acgtggccga aggcctcctc ggaggcgagg ggtcccgtgt
      601 cgcggcctcg ccgatatccg agcgcacgcc gcgcgcggat ccagtacacc tcgtcccact
      661 cgtagctctg gagctcgatg ccggcgcgca cgcgggcctc gggcaggacg cgcacgccgt
      721 cgagctcgcc ccagttggcg agcatcgcgt agtgccgcgc caggccgcgc gcgctcatga
      781 tgccgcccga gccgggaatc gacgcctgct ggacctcgcg ccggttgaac accctggcga
      841 catacgagcc cgccggctcg ccaaccatcc gggcatcgta ctcggccggc gcgggcggcg
      901 cgtcggtgag caccgccacg cgctgcagtt cgcgatcggg gacgccgagg tagatgccgt
      961 tgatgccaag cggcttgcag atctcgtcct gcaggaactg cgcgatcgtg cggccatcga
     1021 cgcggcgcag gatctcgccg ttgatgtagc cgtaattgag cgagtgatac gccgtgcgct
     1081 gaccgatcgg gtacatcggt ttcagcgccg cgatgccgcg acacatgcgg tcccagtcgg
     1141 gcagccagtc gggcgtgtag ccaaccggag tctgcggcac gccggtctgg tgcgacagcg
     1201 catgacgcag cgttgccgct tccttcccgt tctggccgaa ttcgggccag accttcacga
     1261 tcggcatgtc gtagctgatc ttgtgcttct cggccatcac gtgcatgcat gttgcggtga
     1321 cacccttcgt cgttgacgac agcatgaaca tcgtgtcgcc atcgacgggg cgcttcgtcg
     1381 cctcgtcagc catgcccgcc caggcgtcga tgacaagctt gccgcccacg tacgcggcga
     1441 cctgcaggcc gatctctgtc gtgtctttca cgatcgattc gaggaccgcg cgcaccttct
     1501 cgttggcggc gccgagcggc gcctgcgcag atgccggcgt cctcgcgaag gcggccgcag
     1561 ccgcactcat cgtggcggtt tcgagaagct gacggcgggt catggttggg gaaggcacat
     1621 cgtcctcctg tttcgagtaa tcttaggccc gctgtcaaac gggaggacgt gttgcacact
     1681 ggacaaccgg cgcctgccgc ccactcgccg ctgctgacca ggacgtggta ctcctactac
     1741 gtcctcggcg cgctcacgat ctgctacgtg atcaacacca tggatcggtc gcagatcctg
     1801 gcggcgtcgc tccagtcgat caagaaggag ttcgcggcca gcgactttca gatgggcctg
     1861 ctgaccggtg tccccttcgc catcttctat tccgtcatgg gcattccgat cgccgcgtgg
     1921 gccgaccgca ccagccggcg caacgtcctg gcgctcgccg tcgcgagctg gagcgccatg
     1981 accgcaatct tcgggatgtc ggtgaattac gcgatgctgt tcgccgcgcg cgtcggcacg
     2041 gccatcggcg aagccggagg cagcccgccc tcgcattcgc tcatctccga ctactttccc
     2101 aagagccggc gcggcactgc tttcgcaatc ttcgccctcg gggtaccgat cggcacctcg
     2161 ctcggtgcgg cgtacggcgg ctggggcaac cagaacttcg gatggcgtaa cacgttcatc
     2221 ctcgccggcg tgcctggcgt cgtcatcgcg ttgttcgtgt ggctgacggt aaaggagccg
     2281 ccacgtggac tgtcggacgg cgtcgcacgc aagaccgact ccaaggtgcc cggtatgctt
     2341 gaagtgatga gcttcctgtg gcgccgctcc tcgttccgtc atctgtgcct ggcggcggcg
     2401 ctccactccg tggtctggta tgcgagcagc gcgttcaaca acgccttcct gcagcgctcg
     2461 catcagttga cggtcgccga agcgagctac tggatcgccg tccttgccgc gttcgccggc
     2521 ggcggcacgt ttctcggcgg gtggctctcg gaccgtttga gcgccagatc gaacgaccgc
     2581 cgctggtaca tgtgggtgcc cggagtcgcg acgctcctgt gcgtgccgtt tcaattcctg
     2641 gcctttctgt ccacgagcct ggccgtcgcg ctgccgtcgt tcgtcgggct catggtgatg
     2701 gccgccgtgt tcttcgggcc ctccttcgcc atgacgcagg cgctggcgac gcttcgtatg
     2761 cgatcggtgg ctacctcgct cctgctcttc attcagacgc tgatcggctt cgggctcggg
     2821 ccgtcggtca cgggttacat cagcgatcgg ctcgcgccga cttaccaggt cgactccctt
     2881 cggtacgcac tggtcatcat cggcgtcgtc aacctctggg cggcagtgca ttacatgctc
     2941 ggcgcgcgga cgctgaaaca ggacctggag acgacggaga agcttgcgac ggcgtagagc
     3001 gcaggcttga gcctgcgcgt ggccggtctc gctgcatagt ccttggtgct tggttcttgg
     3061 tgcttggtcc gtccttggtc cctggtccgt gcttggtcct tggtcccggg tcctttcaca
     3121 gggcgcggac caaggaccca ggaccaggcg cggacgaagg accgaggacg gacctggtac
     3181 caagcactaa gcaccaagga ctaagatcaa gctgaatgtc agaccagtac gatgtcgtga
     3241 tcgttggcgg cggtgtcgcg ggcctgagtg cggggctgac atcggcgcgc gccgcgcgga
     3301 agacgcgcgt cctgaccggc ccgtcgatcg gcggaaacct gctgagcatc gaacggatcg
     3361 acggctatcc aggctttccg gacggggtcc cgggctacga cctgtgcccg atcatccagg
     3421 agcaggctgt cgcggccggc gcggagttca ccgccgcgga agcaaccggt gttcaggcca
     3481 gcgaaggagg gtggcgcctc accacgagcg ggggcgacgt gtgggcccgc gccgtcgtcg
     3541 ttgccacggg ctccgcgctg aagacgctcg ggattccagg cgaagaacgg ctgacgggca
     3601 agggtgtcag ccactgcgcg agttgcgacg cccctcttct ccggggccgc acggtcggcg
     3661 tcgtcggggg cggagactcc gcgctgcagg aagcgctgac gctggcacag cacgccgcgc
     3721 gcgtgatcgt cctgcatcgc ggcagcgcct tctccgcgca ggaggcctac cgtcagcggg
     3781 tgctcgagaa tccgaagatc gaggttcgct ttggcaccat cgtcgaggaa gcggtcggca
     3841 acggcggact gaccggcgtc cgcacgcgaa gcgcgggcga cgggaagacc ggggacctcg
     3901 aactggccgg gctgttcgtg tacgtcgggc tcgcccccgt caccacgtgg ctcggcagcg
     3961 cgctcgccct cgacgcatcg ggacgggtcc cggttgacgg cgcgatgcgc agcaccctgc
     4021 cgggcctctt cgcggccgga acggtccgat ccggctcggc cggccgggcg gcggcggccg
     4081 ccggcgatgg agcgacggcc gcggtgagcg ccgaccagta cgtgaaagac ggcaaatggc
     4141 gcgcgtgatc gcaagacatc ggtgcggaga cgaggagggt gtatgagaac gctcagtggt
     4201 gcggcggcga tcgccggcct gctcgtggtc ggcgccttgg ctcaggcccc ggagaaaggc
     4261 gggggcgacg tcacgggatc gtatgagctc gtggcgggct ggccacagaa ctggtgcggc
     4321 gagggcttcg tcatcgggtc cacggcgggc atctgggcgg agagcccgga ccgggtgatc
     4381 gtgttcgccc ggggctgtct gccgcggctc gagaatgcgg gcgacgtggt gccgacccgt
     4441 aacgcatccg gattcgatct gtcgcagaag gatccttcac gccatccacg ctgggatcac
     4501 atcgtcaaca tcgtcgaccg caacggccga ttgctggagt cgtgggagca acacaacaag
     4561 ctcttcgtgc ggccacatcg ggtgctggtg agtccctatg acgcggagcg gcacgtgtgg
     4621 ctggtggatg acggcgcgca tgccatctac aagttcacgc gcgacggcaa gcagctcgtg
     4681 atgaccgtgg gcacgccgag gatgccgggc aacgatgaaa cgcactttgc gcggccgacc
     4741 gacgtggcct ttctgcccga cggcacgttc ttcgtcagcg acggttacac caatacccgg
     4801 gtggtgaagt tcgatcgcaa cggcaagtac gtcatgacct ggggcatgcg cggcacgccg
     4861 cccaacgaga cccgacccta ctacatgaac accgtgcacg cggtcgtgac cgacaagcag
     4921 cggcgcgtgt acgtgagcga ccgtgcgaac caccgcattc agatcttcga cgagaacggc
     4981 aagttcctcg acgcctggcc gaacgtcccg ttgccgtatt cgctcctgat gaccgacgat
     5041 caattcctgt ggtccgcgtc gggccagacg cagaaattca ccaagtacga cctgaatggg
     5101 cgtctgctcg ccgcctgggg cacgttcggc gcattcccgg gcggattctg gggggtccac
     5161 cagttccacg tcgatagtga aggaagcctc tacaccgccg acgtacacgt gggacgcccg
     5221 cagaagttcc gtccccgtcc cggcgccgat cgcacccggc tcatcggagc gccgatgaga
     5281 gtcggtgtga cgtctacgcg ctgatgacga cggccggtgg gcggcacagc cagggaggct
     5341 gggtgaggga gaatgacttc tctatgacac aacggatcac ggtgcacgat ccccgcgggt
     5401 acccgccgaa agtggtcggc aagcgtctgg cctcgcggct accgacgctc gatggcaaga
     5461 cggtctatct ggtcgactgt ctgttcgaca attcggccgt gttcatggac cagctgcaac
     5521 agtggttcgg cgagcacctg ccttcggtcg atgcgcgcat catcaggccg aaggagacct
     5581 gggcggacga tccggagatg cgcgcggcca tcgccaagga cggcgacgcc gcgattctcg
     5641 gcatcggtct ttgaagcacc tgcagcccga cggtcgtcgg gctcaccgcg gcgctcgagg
     5701 aaggcggcgt tccctcggtt gcggtacaca cgcagcgatt cgcgcgtctc tgcaaggcga
     5761 cagccctgtc cacggggatg ccgcgcctcc gtcaggcgta cacgccgcac ccggtcgtcg
     5821 atcagcccgc cgagcgtctg cgggcgtaca tcgacggcac cgacgccatt tccgggcgcc
     5881 cgttcatgca ggaagtgctc gacggcctca caacgccgct cgaggacgag gatctccagg
     5941 ggctctcgtt cgagcggacg acgccgcggc tggtggaacc cgcagccgag gacgacctcc
     6001 agcggctgtt cgtggagaac cactggacgg acatgttgcc gatcgtgctg ccgacggagg
     6061 cgcgcgtcga ggcgatgctc aaaggaacga gtcatccgcc ggacaagatc gtcggacggc
     6121 tccgcccgac ggcgttccgc gagtcgtggg aattcgacgt cgagaaagtg gcggtcaacg
     6181 ccgtgatggc tggcgcgcgg ccggcgtact tcccggtcat cctcgcgctg gccgccagcg
     6241 gcatcaccgc caggtccagc agcacgacct cgttcgcgac gatttcggtg atcaacggcc
     6301 cgatccgcaa tgagatcgcg atgaactcgg gcatcggcgt gatgggcccc tacaaccacg
     6361 ccaacgcgac gatcggccgc gcgtacggtc tgctgtcgca gaatctccag ggcgggtcgg
     6421 ttcccaacga gacctacatg ggatcgcacg gcaactggta cgcgtacacg gcggtcatcc
     6481 cggaggcgga ggagcgcagc ccctggcagc cgctgcacgt gcagaagggc ttcaagccgg
     6541 ccgagagcgt tgccttcgtc tttttcggcg gctggtacac gcacgccggt tacggtccgc
     6601 gcgccacctg gaaggagaag atgcggcacg ccctcgcggc ggtggagcag tacagcccgc
     6661 cgctcttcgt gatggacccc atcgtcgcgc gcggattcgt cgatctcgga ttcgacacga
     6721 aagagaagct gatcgactgg tgtgccgaga acgcgctgct gaccgcgcgg gagtactggg
     6781 acaatcaatc gattcaactg ctgcgcccca aagcggtggc cggcatcgag ccgtatgcgt
     6841 cgcgcctcaa ggctcctccg gacgaggtga tcaagatcta cgagccctct gacatccaca
     6901 tcgtcgtcgc cggcggcgag acgcaggggg cgtggaagat gatcagcggc gcgctgcgcg
     6961 ggacagtctc gatcgatgca tggcgatgat cacggtccac gatccgcgcg gctaccctcc
     7021 gaagatcgtc ggcaagcgcc tggcgccgcg gctctcgacg ctcgacggga agacggtcta
     7081 cctggtcgat tgcctgttcg acaactcgga cgtcttcatg gagcagctgc ggcaatggtt
     7141 cgccgagcgg ttgccgtccg tgaagacccg aatcgcccgg ccgaaggaga cgtggtcaga
     7201 cgacgagcag atgcgggcgg cgattcgagc cgacggccac gccgccattc tgggcgtcgg
     7261 cctttgaagc acctgcagcc cgacggtcgt cgggctcgcc gcggcgctgg agcaggacgg
     7321 cgtcccctcc atcgcggtgc acacgcacgt gttcgcacgg ctctgcaagg cgacggcgct
     7381 gtccatgggc atgtcgaacc tgcggcaggc ctacgtgccg cagccggtgg tggaccgttc
     7441 gcccggtgac cttcgcgcct acatcgaagg gcacgatccg gtgtcgaagc ggcccttcat
     7501 gcaggaggtg ctcgacggcc tcacggtccc gccagacgat gcggatctcc aggggctgtc
     7561 gttcgagcgc acgacgccgc ggctcgtgga gcccgacacc gaggacaatc tccgccggct
     7621 gttcatcgac aacaactgga ccgactccct gcccatcgtg ctgccgaccg aagagcgagt
     7681 ggaggcgatg ctcaggggga ccagtcatcc gccggacaag gtcgtggggc ggctccgccc
     7741 gacggcgttc cgtgaaatct gggaattcac ggtggagaag gtggcggtca acgccgtcat
     7801 ggcgggcgca cggcccgagt acttcccggt gatcctcgcg ctcgcctcga gcggcatctc
     7861 cgcgagatcc agcagcacga actcgttcgc caccatttcg gtcgtcaacg gccccatccg
     7921 caacgagatc ggcatgaact cggggatcgg cgtgatgggc ccctacaacc acgccaactc
     7981 cacgatcggc cgcgcctacg gtctgctgtc gcagaatctg cagggtggat cgatcccggg
     8041 cgacacctac atgggatcgc tgggcaactg gctcgcgtac accgcggtgc tgcccgaggc
     8101 cgaggagcgc agtccctggc aaccgctgca cgtgcagaag ggctacaagc cgaccgatag
     8161 cgtcgccagc atcttcttcg gcggctggta cacgcatgcg gggtacggcc cgcgcgccac
     8221 gtggcaggag aagatgcgtc ggtccctcgc cgcggtcgag caatacagcc agcccctgct
     8281 ggtggtggac ccgatcgtcg cgcgcggatt cgtcgatctc gggttcgaca cgaaggagaa
     8341 gctgatcgac tggtgcgccc agaacgcgct gatgacggca cgcgagtact gggacaacca
     8401 gtggattcag tacctcatgc ggccccgcgc ggtggccggc gtggagccgt acgcctcgcg
     8461 cctcaaggcc gaccccgacg agatgatccg gatcttcgag ccatccgaca tccacatcgt
     8521 cgtggccggc ggcgagacgc agggcgcgtg gaagctgctc agcggctcgt tgcgcgccac
     8581 cgtctcgatc gacgcgtggc ggtgaccggc tggacgccgc gccgcttcag agtggccgcg
     8641 tcagtcgcga gatgaccttg tctcagctcc cgcccacctt gcgcggcgtc cgcggtcgaa
     8701 aggcgaagcc acgagtcgcg gtcgccttcc agggctgttg cgtctgatgc ttcggcgcgc
     8761 ggatgggcgt cgaggcggca gcctccgacg gtccagcggg cgtctcggcg gcggccttgg
     8821 cgcgcgctgc cgccgccgcg gcatcatcct tctcgag
//
LOCUS       DBNGSH010000016         4298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4415859, whole genome shotgun sequence.
ACCESSION   DBNGSH010000016 DBNGSH010000000
VERSION     DBNGSH010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4415859"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="AC16CY_00405"
     CDS             <1..725
                     /locus_tag="AC16CY_00405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3352262.1"
                     /translation="HDITDLRRADQIRRDFVANVSHELRTPLTAIRGYVEALLDEPAD
                     AESTRKFLEIVARHSARMERLVKDLLRLARLDARQEPLETARCDVAQIFNAVTADLHP
                     SIEAKRQRIDVDVPAESRVVDGDPAKLHDIVHNLVENAVNYSPEGAEVRLAASRHDGA
                     YTITVADSGPGIPDEDLSRVFERFYRVDKSRSRPGGTGLGLAIVKHLVELHGGKAGAA
                     NRPEGGAIFTITLPTSSEPAGT"
     gene            780..2762
                     /locus_tag="AC16CY_00410"
     CDS             780..2762
                     /locus_tag="AC16CY_00410"
                     /inference="COORDINATES: protein motif:HMM:NF039722.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6311 domain-containing protein"
                     /protein_id="HIR3352263.1"
                     /translation="MSLRGARFHLGVLLAYAATALIFSWPLALHLGSALTGPPDGDTG
                     VYVWNQWVFSHEILVHRRLPYFTSAIFSPGQDANLSLHNYTAFQNLLAMPLTPLFGVV
                     ATFNLVFLLMSVLTAYSTFLLVRHVTGRGAEAWLAGLLFAWSPLLVTRGMGHFSLVAA
                     APLAIFLLVLMKADGHERFRDAIALGAVVAWAACTDVYYGIYCLLIGAVFLGARVLSI
                     HSSPRSGRAAAVRWALDVLLLCATGLVVVIAVTGGRTVTVLGLAVSAHSLYTPVLVLT
                     TLAVTRIAWRLRASVKPTARYDARRLAQLTAGVGLVAAALLSPVLYAVGVRLATLTYT
                     APRVDWRSSPGGVDLLSLVTPNPNHPLAPDWIADWLMSLKAGYLESVVSIPLVVLLTM
                     AIAWKMGWRPSLWWSALALAFGALALGPFVHVGGVNTYIPGPWALLRYVPVLGLARTP
                     ARFSVVMMLAAAVLFATALEWIGRRHPGLRRHLLAVVALLLIAELLPAPLTLHSAAVP
                     RFYEQVAAAPEDVRVLELPTGVSDGTRSVGNFSARYQFFQTAHGKPLIGGYLSRVSED
                     RVATLRKIEVINALMVLSEGGTLPAAGEPHLIESGPAFVRDANLGFVIIDHRRASKSL
                     TDFAVQALRLDEVAAEGALVLYRPRREDPPVESRRP"
     gene            3017..4288
                     /locus_tag="AC16CY_00415"
     CDS             3017..4288
                     /locus_tag="AC16CY_00415"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR3352264.1"
                     /translation="MRQTSDTLAIILGGGQGSRLFPLTATRSKPAVPIGGKYRLIDIP
                     ISGCLHADIRRIFVLTQFNSASLNRHISNTYQLDHFSGGFVEILAAEQTPDNPNWYQG
                     TADAVRQATRHFSQHAAEFDLILAGDHLYRMDYRDLVEAHKRQGADITIAAQPVDAGT
                     ATGMGIFLFDTSGNIVAFEEKPKPPRLEEIGRSIPAGSTFAEHSDAQPFMASIGVYVF
                     TRRVLLDMLEHDPGHDFGRALIPNALGKYRVRPYLYRGYWADVGTIESFYQTNVMLGR
                     LDSPFHFWHPERPIYTHLRQLPASLMPDAHVRDSIIAEGCYLSGCEVEGSVVGVRSAV
                     REGSRITRSVLLGADFYEYGAPPDHLPGLGIGRGVVLDRVIVDKNARIGDHARLVNEQ
                     QVAAADGDGYYIRGGIIVVPKGGVIRGGTIV"
BASE COUNT          646 a         1494 c         1391 g          767 t
ORIGIN      
        1 tgcacgacat caccgacctg cgccgtgccg atcagatcag gcgggacttc gtcgccaacg
       61 tgtcgcacga gctgcggacc ccgctgacgg cgatccgggg atacgtcgag gcgctgctcg
      121 acgagccggc cgacgcggag agcaccagga agtttctcga gatcgtggcg cgccactcgg
      181 cgcgcatgga gcggctcgtc aaggatctgc tgcgcctcgc caggctcgac gcccggcagg
      241 agccgctcga gaccgcccgc tgcgacgtcg cgcagatctt caacgccgtc acggccgatc
      301 tccatccatc gatcgaagcc aagcgccagc gcatagacgt ggacgtgccg gccgagtccc
      361 gggtggtgga cggcgatccg gccaagctcc acgacatcgt ccacaacctg gtcgagaacg
      421 cggtgaacta ctcgccagag ggcgccgagg ttcgcctcgc cgcatccagg cacgacggcg
      481 cctacacgat cacggtggcc gattcgggcc cgggtattcc cgacgaggat ctgtcgaggg
      541 ttttcgagcg cttctaccgc gtggacaagt cccggtcgcg ccctggcggc acaggccttg
      601 ggctcgcgat cgtgaagcac ctggtggaac tgcacggcgg aaaagccggg gcggcgaacc
      661 gtcccgaagg cggggcgatc ttcaccatca ccctgcctac ctcctccgaa cccgccggca
      721 cgtagggcgg acctggccct caccgggccg gttttctggt atgctcccgt ccgctaaaga
      781 tgagcttgcg cggcgcgcgg tttcatctcg gcgtgcttct cgcctatgcg gcgacggccc
      841 tgatcttttc ctggccgctc gcgcttcatc tcggttcggc cctgacggga cctccagacg
      901 gtgacaccgg cgtgtacgtc tggaaccagt gggtgttcag tcacgagatc ctcgttcacc
      961 ggaggcttcc gtactttacg agcgcgatct tttcgccagg acaggacgcg aacctcagcc
     1021 tccacaacta cacggcgttc cagaacctgc tggccatgcc cctgacgccg ctgttcggtg
     1081 tggtggccac cttcaacctg gtcttcctgc tgatgtccgt cctgacggcg tactcgacct
     1141 ttctcctggt acggcacgtg acgggacggg gggccgaggc atggctggcc ggccttcttt
     1201 tcgcgtggag cccgctgctc gtgacccgag gcatgggaca tttcagcctc gtggccgccg
     1261 cgccgctcgc gatttttctg ctcgtcctca tgaaagccga cgggcacgag cgatttcgcg
     1321 acgcgatcgc gctcggcgcc gtcgtcgcgt gggccgcctg cacggacgtg tactacggga
     1381 tctactgcct gctcattgga gcggtgtttc tcggcgcgcg agtcctgtcc atacacagca
     1441 gcccacggtc cggacgagcc gccgctgtca ggtgggcgct cgacgtgctg ctgctctgtg
     1501 cgacgggcct cgttgtcgtg atcgcggtga cgggcgggcg aacggtcacg gttctgggac
     1561 tcgcggtctc ggctcatagc ctctacacgc cggtgctcgt cctgacgacg ctcgccgtga
     1621 cgcgtatcgc gtggaggctg agggcctcgg tcaagccgac cgccaggtac gatgcgcggc
     1681 ggcttgctca gttgacggcg ggcgtcgggc tcgtcgccgc cgcgttgtta tcgccggtgt
     1741 tgtacgccgt cggtgtgcgg ctcgcgacgc tgacctacac cgcgccgcgg gttgactggc
     1801 gatcgagccc tggcggagtc gacctgttgt cgctcgtcac gccgaatccg aaccatcctc
     1861 tcgcccccga ctggatcgcc gactggctga tgtcgctgaa agcgggctat ctcgagagcg
     1921 tcgtctccat tccgctcgtc gtgctgttga cgatggcgat cgcctggaag atgggatggc
     1981 gtccgtccct gtggtggtcg gcactcgcgc tcgcattcgg ggcgctcgcg ctcggcccgt
     2041 tcgtccacgt cgggggcgtc aacacgtaca tcccgggacc atgggcgctg ctccgctacg
     2101 tacccgttct cgggctcgcg cgaacgcccg cccgcttctc agtggtgatg atgctggccg
     2161 ccgccgtcct gttcgcgaca gcgctcgagt ggatcggccg gcgtcacccg ggactccggc
     2221 ggcacctgct cgccgtggtc gcactgctgc tgatcgcgga gctgctgccg gcgccgctca
     2281 cgctgcactc ggcagccgtg cctcgtttct acgagcaggt cgcggcggcc ccggaggacg
     2341 tgcgtgtgct cgaactgccc accggcgtca gcgacggaac gcggagcgtg ggcaacttct
     2401 cggcgcgcta tcaattcttc cagacggctc acggcaagcc gctcatcggc ggatacctgt
     2461 cgcgcgtttc cgaagatcgc gtggcgacgc tgcgcaagat tgaagtgatc aacgcgctga
     2521 tggttctcag cgagggaggc acgctgcccg ccgccggcga gcctcatctc atcgagagcg
     2581 gtcccgcgtt cgtccgcgac gccaacctcg gcttcgtgat catcgatcac cggcgcgcct
     2641 cgaagagctt gaccgacttt gccgttcagg cgttacggct ggacgaggtc gccgccgagg
     2701 gcgcgctcgt cctctaccgg ccgaggcgcg aggatccgcc cgtcgagtca cgccgcccgt
     2761 gatccgttcc tgaacagcgt cagcacgatc tcgacgcggg cgcaggcaat aacgctgaac
     2821 ctcatcagcg ccacgcctgc gcctgtggtg ccagcttgtc gccgtcgcac ccgcgtgcgc
     2881 ggcgactacc cccgttgccg tggtataccc acgtgcatac gtcaatcagc ctaaagtgct
     2941 gctgtgacgc ggggatcacg atcggcacac gtccgtgtta aatacgcgtc cagacggagg
     3001 attgcgcgga accgccatgc gacagaccag cgacaccctc gccatcatcc tcgggggcgg
     3061 tcagggctcg cgcctctttc ccctgacggc tacccgttcc aagccggccg tgcccatcgg
     3121 gggcaagtac cgcctgatcg acattccgat cagcggctgc ctgcacgccg acatccgccg
     3181 catcttcgtc ctgacgcagt tcaactcggc ctcgctcaat cggcacatct cgaatacgta
     3241 ccagctcgat cacttctcgg gcgggttcgt cgagatcctc gcggccgagc agacgccgga
     3301 caacccgaac tggtaccagg gcacggcgga cgcggtgcgc caggccacgc gccatttcag
     3361 ccagcacgcc gccgaattcg acctcattct cgccggggat catctctacc ggatggacta
     3421 ccgggatctc gtcgaggcgc acaagcggca gggcgcagac atcacgatcg ccgcgcagcc
     3481 ggtggacgcg ggtaccgcga ccggcatggg catcttcctc ttcgacacga gcggcaacat
     3541 cgtcgccttc gaggagaagc cgaagccgcc gcgcctcgag gagatcggcc gcagcattcc
     3601 cgccggctcc acgttcgccg agcacagcga cgcgcagccc ttcatggcct ccatcggcgt
     3661 ctacgtgttc acaaggcgcg tgctactcga catgctcgag cacgatccag gtcacgattt
     3721 cggacgcgcg ctcattccga acgcgctcgg caagtaccgc gtccgtccgt atctctaccg
     3781 cggctactgg gccgacgtcg gcacgatcga gtccttctac cagaccaacg tgatgctggg
     3841 gcgtctcgac tccccgtttc atttctggca tccggagcgc cccatttaca cccacttgcg
     3901 gcagttgcct gcctcgctca tgccggacgc ccacgtgcgc gactcgatca tcgcggaggg
     3961 ctgctacctc tcgggctgcg aggtggaggg ctcggtggtc ggcgtgcgct ccgccgtccg
     4021 cgagggctcg cgcatcacgc gctcggtgct gctcggagcc gatttctacg agtacggcgc
     4081 cccgccagat cacctccccg gcctcggcat cggacgcggc gtcgtgctcg atcgcgtcat
     4141 cgtggacaag aacgcgcgga tcggcgacca cgcgcggctc gtgaacgagc agcaggtggc
     4201 cgcggccgac ggcgacggct actacatccg gggaggcatc atcgtggtgc cgaagggcgg
     4261 cgtgatccgg ggggggacga tcgtgtaggg ggcagggg
//
LOCUS       DBNGSH010000017         3558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3906541, whole genome shotgun sequence.
ACCESSION   DBNGSH010000017 DBNGSH010000000
VERSION     DBNGSH010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3906541"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="AC16CY_00420"
     CDS             complement(<1..256)
                     /locus_tag="AC16CY_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3352265.1"
                     /translation="MYGLGLGAGLPLILDRTSTVLAAEALQGTSVERHPNRILVVLEL
                     SGGNDGLNTVVPFGDPAYYRARPTLAIAERETIKVAGGFGF"
     gene            complement(309..2120)
                     /locus_tag="AC16CY_00425"
     CDS             complement(309..2120)
                     /locus_tag="AC16CY_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HIR3352266.1"
                     /translation="MIRGSHRQVLLVVLSCFCALAASHARVAAQSDAWAGDLSPIPAG
                     AWNYDRAAHLIERAGFGATPEEIARLAAMTPQRAVASLVDYDAIDASALKPFDESGIW
                     DPGMDPFPPSRAEAVRLARERGEGLGERVLPAGTPRRLQPVVDKFFYSLTANNIETQR
                     LGLWWANRMLTTPRPLEEKLTLFWHGHFATAQNKVRDSRMMYQQNRMLRTNASGRLRD
                     LLVGILQDPAMLVYLDNGENVKSHPNENFGRELLELFTMGVGNYAERDVREAARAFTG
                     WTNDVLTFTFHAPQHDFDQKTFLGRTGPLNGQDVIDIILAQRVTGEFVAAKLYRYFVR
                     EDVPNAVRTDLGRSFRESGYQIKPLLRRIFLSKDFYSNVSTATQIKSPVHLVVSTYRK
                     MGLADVPTIPDFGRMTGSLGQTLFEPPNVAGWAGGRTWITPATLLQRGNLFRDVLFPD
                     TKGFRPPDRTMSPTDTRVGERLAKGMNITEATVEGEAQSNKMVDRDEDYNTRYGGYAG
                     NLLAFKRTKLIPRHAAAIDLTAMVKAAGADTPEEVVDHFVHRFLRVTLADKDRAALVE
                     FFRGKLGSSSIRPSATLESSLRELLYLVLSTPEYQLG"
     gene            complement(2183..3202)
                     /locus_tag="AC16CY_00430"
     CDS             complement(2183..3202)
                     /locus_tag="AC16CY_00430"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3352267.1"
                     /translation="MTRTSIAALVAVTLAAAFSDRVSSQSPTGTISGVVSDPDGAVFP
                     GAFVQARGAREYNEASDDAGQYRLQVVPGTYDVSINVPGMKGFQRKGVVVQANQNVVI
                     DARLEDTASLRTLGEDPASIAARFFTRPEPPKGRTPRLSNRKPDLSGMWLSGPADLDT
                     LPMLPWAAALAKERADAHMKDFPPTYCLPSGPIPLLDPGHFKIVHHPATLLMLFEGDT
                     PGFRQVFLDGRPHPKDADPLWLGHSIGRWEKDTLVIESIGFRDRGWLDFEGRPHSDKL
                     RVTQRLSRPDLGHLNIEITIDDPGALQKPWKTTKGAQLDPGEEILESICTENNKAPGL
                     ISGSK"
     gene            3372..>3558
                     /locus_tag="AC16CY_00435"
     CDS             3372..>3558
                     /locus_tag="AC16CY_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352268.1"
                     /translation="MLRGLASLLILACTPVLAAAQTSDGGIGTALSPSMPASVQSMHA
                     TIRRNLAEAAESMPAAEY"
BASE COUNT          566 a         1165 c         1193 g          634 t
ORIGIN      
        1 ggaagccgaa gccgccggcc accttgatgg tctcgcgctc cgcgatcgcg agcgtcggcc
       61 gcgcgcggta gtacgccgga tcgccgaacg ggacgacggt gttcaggccg tcgttgccgc
      121 cggacagctc gaggacgacg aggatgcggt tgggatggcg ctcgacgctg gtcccctgca
      181 gcgcctccgc ggcgagcacg gtggacgtgc gatccagaat gagcggcagt ccggcaccga
      241 ggccgagccc atacatacct cgagcaatga attcgcggcg ggaacatccg catttcagca
      301 tttggacctc aacccaactg atactcgggc gtactcaata ccagatacag cagctcgcgc
      361 agggacgact ccagcgtcgc gctcggccgg atggacgacg acccgagctt gccgcggaag
      421 aactcgacca gcgccgcgcg gtccttgtcc gcaagcgtca ctcgaagaaa cctgtggacg
      481 aagtgatcga cgacttcctc gggcgtgtcg gcgcccgccg ctttcaccat cgccgtcagg
      541 tcgatcgcgg ccgcgtgccg ggggatcagc ttcgtccgct tgaaggcgag caggttgccg
      601 gcgtatccgc cgtagcgcgt gttgtagtcc tcgtcgcgat cgaccatctt gttcgactgg
      661 gcctcgcctt cgaccgtggc ctcggtgatg ttcatcccct tggccagccg ctcccccacc
      721 cgcgtgtcgg ttggcgacat cgtgcgatcg ggaggtcgga agcccttggt gtcgggaaag
      781 agcacgtcgc ggaacaggtt gccgcgctgc aggagcgttg ccggcgtgat ccacgtccgg
      841 ccgccagccc acccggcgac gttcggcggc tcgaacagcg tctggcccag gctgccggtc
      901 atccgtccga agtcggggat ggtggggacg tccgcgaggc ccatcttccg gtaggtcgac
      961 acgaccagat gcaccgggct cttgatctgt gtcgcggtgg agacgttgct gtagaaatcc
     1021 ttcgacagga agatgcgcct caggagcggc ttgatctgat agccgctctc gcggaacgag
     1081 cgtcccaggt ccgtgcggac ggcgttcggc acgtcttcgc gaacgaagta gcgatagagc
     1141 ttcgccgcga cgaactcgcc cgtcaccctc tgcgcgagga tgatgtctat gacgtcttgg
     1201 ccgttgagcg gtccggtacg gcccaggaac gtcttctggt cgaagtcgtg ctgcggcgcg
     1261 tggaacgtga acgtcagcac gtcgttggtc caccccgtga acgcacgcgc ggcttcgcgc
     1321 acgtcgcgtt cggcgtagtt gccgacgccc atcgtgaaga gctcgagcag ctcgcggccg
     1381 aagttctcgt tcgggtggct cttgacgttc tcgccgttgt ccaggtacac gagcatggcc
     1441 ggatcctgga ggatcccgac gagcagatcg cgaagccgcc ccgacgcgtt ggtgcgcagc
     1501 atccggttct gctggtacat catccgggag tcccggactt tgttctgtgc cgtcgcgaaa
     1561 tggccgtgcc agaagagcgt cagcttctcc tcgagcggcc gcggcgtcgt gagcatccgg
     1621 ttcgcccacc acagcccgag gcgctgcgtc tcgatgttgt tcgccgtcag gctgtagaag
     1681 aacttgtcca ccaccggctg gagccgccgc ggtgtgccgg cgggcagcac tctctcccca
     1741 aggccctcgc ctctctcgcg cgccagccgt acagcttccg cgcggctcgg gggaaatgga
     1801 tccatgcccg gatcccagat gcccgattcg tcgaacggct tcagcgcgct cgcgtcgatc
     1861 gcatcgtagt cgacgaggct ggcgacggcg cgttgaggcg tcatggcggc aaggcgggcg
     1921 atctcctcgg gcgtcgcgcc gaagccggcg cgctcgatga ggtgcgcggc gcggtcgtag
     1981 ttccacgcac cggccgggat cggcgagagg tcgcccgccc acgcatcgga ctgagcggcg
     2041 acgcgcgcat ggctcgcggc gagggcgcag aagcaggaga ggacaacgag cagaacctgg
     2101 cgatgcgatc cgcggatcat ggcactctcg tccccggccg attatacgcc ccggccggct
     2161 gaccgctcgt ctcgccctcc ggctacttgc ttccggagat gagcccgggc gctttgttgt
     2221 tctcggtgca gatggactcg agtatttctt caccaggatc gagctgcgcg cccttcgtcg
     2281 tcttccacgg cttctggagc gcgccgggat cgtcgatggt gatctcgatg ttcagatgac
     2341 caagatccgg acggctgaga cgctgcgtca cgcgcagctt gtcggagtgc ggacgccctt
     2401 cgaaatcgag ccagccgcgg tcgcggaagc cgatcgactc gatcacgagc gtgtctttct
     2461 cccaccggcc gatcgagtgg cccagccaca gcggatcggc atccttcggg tgagggcgcc
     2521 catcgaggaa cacctgccgg aaccccggcg tgtcgccctc gaagagcatc agcagcgtgg
     2581 ccgggtggtg cacgatcttg aagtgcccgg ggtcgagcag cggaatcggt cccgatggca
     2641 gacagtacgt cggggggaaa tccttcatgt gggcatcggc gcgctctttg gccagcgccg
     2701 cagcccacgg cagcatgggg agcgtgtcga gatcggccgg cccggagagc cacatcccgg
     2761 agagatccgg tttgcgattg gacaatcgcg gcgtgcgtcc tttcggcggc tccgggcggg
     2821 tgaagaacct cgcggcgatg gacgctggat cctcgccgag cgtccgcagc gacgcggtgt
     2881 cttccaggcg ggcgtctatc acgacgttct gattcgcctg cacgacgacg cccttgcgct
     2941 gaaagccctt catccccggc acgttgatcg acacgtcgta ggtaccgggc accacctgca
     3001 gcctgtattg gcccgcgtcg tcgctggcct cgttgtattc cctggcgccc cttgcctgca
     3061 cgaaggctcc cgggaacacg gcgccgtcag ggtcgctcac gacgccgctg atcgtgccgg
     3121 tcgggctctg agaggacact cgatccgaga acgccgccgc aagggtcact gcgacaagcg
     3181 ccgcaattga tgtacgggtc atgcggacac tccttacacc tacgcgttgg gcgattatac
     3241 ggggcagtac gtcgtgtccg gctttatccg gaccgtgacg cagtgtccgg cttcagccgg
     3301 acccgggtag actgatgccc tacacaactg gaggtccggc tgaagccgga cactacgtga
     3361 ggagacgtca tatgttgcgc gggctggctt cgctgctgat tctggcgtgt acccccgttc
     3421 tggccgcggc gcagacgagc gacggcggca ttggcactgc cctctcgccg tcgatgcctg
     3481 cctccgtgca gtcgatgcat gccacgattc ggcgcaacct ggccgaggcg gccgaaagca
     3541 tgcctgccgc cgagtacg
//
LOCUS       DBNGSH010000018         2896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1385, whole genome shotgun sequence.
ACCESSION   DBNGSH010000018 DBNGSH010000000
VERSION     DBNGSH010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1385"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..968)
                     /locus_tag="AC16CY_00440"
     CDS             complement(36..968)
                     /locus_tag="AC16CY_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352269.1"
                     /translation="MKQMLLGGAILAMVATVHAAPQQGDGNRPADQPAPRTADGRIVI
                     GATPTQKGVWIGGNLGFCNANRAPAPASLNPGATGGPGAVTPPAPAGAGPAGAGPAGQ
                     AGRAGGRGRGAAGPCTPVPYMEWTLAVSDDRRRNELEPHTRCKPSGGPRQWLTPYGAE
                     FLELPEQKVAYIFDIGGPHTYRTIFMDGRAHPKDLAPSFYGHSIGWWEGDTLVVDTVG
                     FNEGFWIDRGQLPHTDKLHLIERYTRTSLTSMRYELTIDDPAAYKAPFTATSNLRWEN
                     GTELFEYICQQANQAHELMVGAGTEVDRSTLIIP"
     gene            1089..>2896
                     /locus_tag="AC16CY_00445"
     CDS             1089..>2896
                     /locus_tag="AC16CY_00445"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR3352270.1"
                     /translation="MKVEEGSLLWTPSASRVERSHLTRYLRWLAGRGLTFDDYEALWR
                     WSVSDLEAFWSSIAEFCGLQFSRPPSRVLGKRTMPGAEWFPGAALNYAQHALRHERPG
                     ENALLYLSERRPLDALSWPELARQVRVLATRMRALGIAPGDRVVAYLPNSPEAIVGML
                     ATASIGAVWSSCGPDFGTRGVLDRYAQLAPKLMFCVDGYSYGGKPFDRRSDVREIIGQ
                     LPTLSHVVHVPYLDLSRQSADGAAADRPARTPITPGALLWPDVLAGPDPGREAFQFEQ
                     VPFAHPLWILFSSGTTGLPKPIIHCHGGITIEQLKLFHFHMDMHPRQRMFFFTSTGWM
                     MWNFLASSLIVDVVPVLYDGNPAWPSPDLLWKLADDTGAHLFGASPSYQAILEKAGIV
                     PRERFALESLETIVLAGSPVTAECQNWFFENVKKDLWAHSGSGGTDICTGLVGGVVNL
                     PIYAGEIQARQLGVAAYAYDERGKAIVDQVGELVLTEPMPSMPVGFWGDTDGSRYLES
                     YFDQYPGIWRHGDFFRVNARGGCFVLGRSDATLNRHGVRIGTAEVYRSLLGVPAVEDS
                     LIVNLDLPGGGFFMPLFVKTRNNVTLDESIGDEIRAR"
BASE COUNT          430 a          977 c          966 g          523 t
ORIGIN      
        1 ggaccaagca ccaaggacta agggccaagc actaactagg ggatgatcag ggtactgcgg
       61 tcaacctcgg tgccggcgcc gaccatcagt tcgtgcgcct ggttcgcctg ctgacagatg
      121 tattcgaaga gctccgtgcc gttctcccag cgcaggttcg acgtcgccgt aaacggcgcc
      181 ttgtaggcgg cggggtcgtc gatggtcagc tcgtatcgca tcgacgtgag cgacgtgcgc
      241 gtgtagcgct cgatcaaatg cagcttgtcg gtgtgcggca gctggccgcg atcgatccag
      301 aagccttcgt tgaaaccgac cgtgtccacg accagcgtgt ccccctccca ccagccgatc
      361 gagtggccgt agaagctcgg cgccaggtcc ttcggatggg cacggccgtc catgaatatc
      421 gtgcggtagg tatgcgggcc gccgatgtcg aagatgtagg cgaccttctg ttccggcagc
      481 tcgaggaact cggcgccgta cggcgtcagc cactggcgcg gcccgccgga cggtttgcag
      541 cgcgtgtgcg gctcgagctc gtttcgacgg cgatcgtcgg agaccgccag ggtccattcc
      601 atgtagggga ccggcgtgca gggcccggcc gcgccacggc ctcgccctcc ggcacggccg
      661 gcctggcccg caggtcctgc gcccgcaggt cccgcacccg ctggtgccgg gggagtgacg
      721 gcgcccgggc ccccggtggc gcccggattg agagacgccg gcgccggcgc cctgttggcg
      781 ttgcagaagc cgagattgcc gccgatccac acacccttct gcgtcggcgt ggcgccgatg
      841 acgatgcggc cgtccgccgt gcgcggcgct ggctgatccg caggacggtt cccgtcgccc
      901 tgttgtggcg cggcatgcac ggttgccacc atcgccagga ttgcaccgcc gagaagcatc
      961 tgtttcatgc gcgaatccta acacgtaaca cggcgaggga cccgccttgt tgggccggag
     1021 cccccggctc gaagctcgtt gttcgcggta ctcttccagt tgaaaccgtc cagtagactg
     1081 atctctgcat gaaggtcgaa gagggctcgc tgctctggac tccgtcggcg tcgcgcgtcg
     1141 aacgttcgca tctcactcga tacctgcgct ggctcgccgg tcgcggcctg actttcgacg
     1201 actacgaggc gctgtggcgc tggtcggtct ctgacctcga ggcgttctgg agctcgattg
     1261 ccgagttctg tggcctgcag ttctcgaggc cgccctcccg cgtgctcggg aaacggacca
     1321 tgccgggggc cgagtggttt cccggcgccg cgctcaacta cgcccagcat gcgctgcgtc
     1381 acgagcgccc gggcgagaac gcgctgctgt acttgtccga gcgacggccg ctggacgcgt
     1441 tgtcgtggcc cgagctggcg cgccaggtgc gggtgctcgc cacgcgcatg cgcgcgctcg
     1501 gcatcgctcc gggcgatcgc gttgtcgcgt atctgccgaa ttcgcccgaa gcgatcgtcg
     1561 gaatgctggc gaccgccagc atcggcgcgg tgtggtcgag ctgcggtccc gactttggca
     1621 cgcgcggagt gctcgatcgt tacgcgcagc tcgcgccaaa gctcatgttc tgtgtcgatg
     1681 gctacagcta cgggggcaag ccgttcgaca ggcgctccga cgtccgcgaa atcatcggtc
     1741 agctcccgac gctctcgcac gtggttcacg ttccgtatct ggacttgtcg cgccaaagcg
     1801 ccgacggcgc ggcggcggac cggcccgctc ggacgcccat caccccgggc gcgctcttgt
     1861 ggccggacgt gctcgcgggt cccgatccgg gccgcgaggc gtttcaattc gagcaggtgc
     1921 cgttcgcgca tccgctgtgg attctgttct cgtccggcac caccggcctt cccaagccga
     1981 tcatccactg ccacggcggc atcaccattg agcagttgaa gctgttccac ttccacatgg
     2041 acatgcatcc ccgccagcgg atgttcttct tcacgagcac gggctggatg atgtggaact
     2101 tcctggcgag cagcctgatc gtggacgtcg tgcccgtgct ctacgacggc aacccggcgt
     2161 ggccctcgcc cgacctgctc tggaagctcg ccgacgacac gggcgcgcac ctgttcggcg
     2221 ccagccccag ctaccaggcc atcctcgaga aggccggcat cgtgcccagg gaacgcttcg
     2281 cgctcgaatc tctcgaaacg atcgtgctcg cgggctcgcc ggtcaccgcc gagtgccaga
     2341 actggttctt cgagaacgtg aagaaagacc tgtgggcgca ctccggcagc ggcggcaccg
     2401 atatctgcac cggactcgtc ggcggcgtgg tcaacctgcc gatctacgcg ggggagatcc
     2461 aggcgcgtca gctgggtgtg gcggcgtacg cctacgacga gcgtggcaag gcgatcgtcg
     2521 atcaggtggg cgagctggtg ctcaccgagc cgatgccgtc catgcccgtc ggcttctggg
     2581 gcgacaccga tggcagccgg tatctggagt cgtacttcga tcagtatccc ggcatctggc
     2641 gccacggcga cttcttccgc gtgaacgcgc gcggcggctg cttcgtgctc ggccgctcag
     2701 acgcgacgct caatcgccac ggcgtgcgca tcggcacggc ggaggtctac cgctcgcttc
     2761 tcggcgtgcc ggccgtcgaa gactcgctca tcgtcaatct ggatcttccc ggcggcgggt
     2821 tcttcatgcc gctgttcgtc aagaccagga acaacgtgac gctggacgag agcatcggcg
     2881 atgagatccg cgcccg
//
LOCUS       DBNGSH010000019         2612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_340861, whole genome shotgun sequence.
ACCESSION   DBNGSH010000019 DBNGSH010000000
VERSION     DBNGSH010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_340861"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="AC16CY_00450"
     CDS             <1..1034
                     /locus_tag="AC16CY_00450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3352271.1"
                     /translation="MRSAVSTGVVFTLAWLCAIGIDARVTGAAAPGAQSQPVAVADAS
                     GLEIIQLRSNFFLIAGAGGHIGVQTGEDGVVVVDAGSADKSPAVLAAIRRLSPRPIRY
                     IIDTSADADHVGGNEALAKAGQTLFTNAGSIALPGDFIGGAASILAAEGVLRRMSAPT
                     GQASPYPGAAWPTETFDHARKYMYLNGEGIEVLHQPAAHTDGDVMVFFRRSDVVVAGD
                     ILDTTRFPAIDVARGGSIQGEIAALNRLVDLAIPSVPIVSREAGTIVVPGHGRLCDQF
                     DVVEYRDMVTIVRDRVQELVDAGRSLDQVKAASPARGYTRRYGGDSGAWTNQFVEAVY
                     ASLTKGKKR"
     gene            1031..1786
                     /locus_tag="AC16CY_00455"
     CDS             1031..1786
                     /locus_tag="AC16CY_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352272.1"
                     /translation="MSTGRLVAIALVAAASLVRPVGVSGQGRGGPPATPRASAPIELT
                     GYWVAFVTEDWRYRMVTPAKGDYRGIPITLEALKIVNAWDPAADEAAGNACKSYGAGA
                     VMRLPGRIHITWQDDNTLKLDTDAGTQTRLFRFAAQSGPPGPPTWQGDSAARWEVAGG
                     RGGAARAGSLTVVTTNMRAGYLRKNGVPYSESARVTEYFDLASQSNGPPLLVVTTVVD
                     DPRYLTQQFIVSSHFKKEADGSKWDPTPCSAAW"
     gene            1753..>2612
                     /locus_tag="AC16CY_00460"
     CDS             1753..>2612
                     /locus_tag="AC16CY_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352273.1"
                     /translation="MGSNTLFGGVVIARTSLAVLALLSAPMLGAAYAQSELAGSWAAR
                     NTEDISRDSYPVDYVGLPLNEEGRTRALAYNESQLAMIERQCEGWPAFYYVQGPFGLK
                     IWSDTDPIRGGVISYTIGAWEDKAPMTIWMDGRPHPSKYAEHTRGGFSTGRWEGTTLV
                     ATTTHMKAGFLRKNGPPSSDQATMTSRFYRHGDILTVLVVIEDPIYLAEPALFSKSFQ
                     LSAAPVSPIGPPCVTTFEGRPVGESAPHFTPEKNPFVDELTKMFNLPREAVLGYAETL
                     YPEYRKKIKDT"
BASE COUNT          441 a          849 c          918 g          404 t
ORIGIN      
        1 ccatgagatc tgccgtcagc accggcgtcg tgttcaccct cgcatggctg tgcgccatcg
       61 gcattgacgc gcgcgtgacg ggcgcggcgg cgcccggcgc gcaaagccag ccagtcgccg
      121 tcgccgatgc gagcggcctc gagatcatcc agcttcgctc caacttcttc ctgattgccg
      181 gagcgggcgg ccacatcggc gtccagacgg gcgaagacgg cgtcgtcgtc gtggacgccg
      241 gctccgcgga caagtctcct gcggtactgg cggcgatcag gcggctgtcg ccgaggccca
      301 ttcgctacat catcgacacc agcgcagatg ccgaccacgt gggcgggaac gaggcgctcg
      361 cgaaggccgg gcagacgctc ttcaccaacg cgggatcgat cgcactgccg ggcgacttca
      421 tcggcggcgc ggcgtcgatt ctggcggcag agggtgtcct gcggcggatg agcgcgccga
      481 cggggcaggc ctcgccgtat cccggggctg cgtggcccac cgagacgttc gaccacgcgc
      541 ggaagtacat gtacctgaac ggcgagggga tcgaggtcct ccatcagccg gccgcgcaca
      601 ccgacggcga cgtgatggtg ttctttcgcc gctccgacgt cgtcgtcgcc ggcgacattc
      661 tggacacgac gcgctttccg gcgatcgacg tggcccgagg cggcagcatt caaggcgaga
      721 tcgcggcgct caaccggctg gtggacctgg cgattccttc ggtgcccatc gtttcgcgcg
      781 aggcggggac gatcgtcgtt cccggtcacg gcaggctctg cgatcagttc gacgtggtcg
      841 agtaccgaga catggtgacc atcgttcgcg atcgcgtgca ggagctcgtg gacgcgggac
      901 ggtcgctgga ccaggtcaag gccgcatcac ccgcgagggg atacacgcga cgttacggcg
      961 gcgattcagg ggcctggacc aatcagttcg tcgaggccgt gtacgccagc ctcacgaagg
     1021 ggaagaagcg atgagcaccg gacggctcgt tgcaatcgca ctggtggctg cggcctcgct
     1081 cgtgcggccc gtcggagtgt cgggccaggg tcgcggcgga ccgccggcga cgcccagggc
     1141 gtcggcgccg atcgagctga ccggctactg ggtggctttc gtcaccgaag actggcgcta
     1201 tcgcatggtc acgcccgcga aaggggacta tcgcggcatc ccgatcacac tcgaggcgct
     1261 gaagatcgtc aacgcctggg atccggcggc cgacgaggcc gcgggcaacg cctgcaagtc
     1321 gtacggcgcg ggagcggtga tgcggctgcc gggacgcatt cacatcacgt ggcaggacga
     1381 caacaccctg aagctcgaca ccgatgcggg cacgcaaacg cggctctttc gttttgcggc
     1441 gcagtcgggc cccccgggtc cgccgacctg gcagggcgac tcggccgcgc ggtgggaggt
     1501 tgccgggggc cgcggcgggg cggcacgggc gggatcgctc acggtggtga cgacgaacat
     1561 gcgggcgggg tacttgcgca agaacggcgt gccgtacagc gagagcgcgc gcgtgacgga
     1621 gtacttcgat ctcgcctcgc agtcgaacgg ccctcctctg ctcgtggtca cgactgtcgt
     1681 ggacgaccct cgatacctga cacagcagtt catcgtcagc tcgcacttca agaaggaagc
     1741 ggacggttcc aaatgggatc caacaccctg ttcggcggcg tggtgatcgc gcggacgtct
     1801 ctggcggtac tggcgctgct gtcggcgccg atgctcggcg ccgcctatgc gcagtcggag
     1861 cttgcgggat cgtgggccgc gcggaacacc gaggacatct cacgcgattc gtaccccgtg
     1921 gactacgtcg gcctcccact caacgaggaa ggcaggacga gggcgctggc ctacaacgaa
     1981 tcgcagctcg cgatgatcga gcggcagtgc gaggggtggc cggccttcta ctacgtgcag
     2041 ggcccgttcg gactgaagat ctggagcgac accgacccga tcaggggcgg cgtgatctcg
     2101 tacacgattg gcgcgtggga agacaaggcg ccgatgacga tctggatgga cggccgcccg
     2161 cacccgtcga agtacgccga gcacacgcgc ggcggctttt ccaccggccg ctgggagggg
     2221 acgacgctcg tcgccaccac gacgcacatg aaagccggat tccttcgcaa gaacggcccg
     2281 cccagcagtg accaggcgac gatgacctcg cgcttctacc ggcacgggga catcctgacc
     2341 gtgctggtcg tgatcgagga tccgatctac cttgccgagc cggcactctt ctcgaagagc
     2401 tttcagctct ccgccgcgcc ggtctcgccc atcggcccgc cgtgcgtgac gacgttcgag
     2461 gggcggccgg tgggggagag tgctccgcac ttcacgcccg agaagaaccc gttcgtcgac
     2521 gagctgacga agatgttcaa tctcccgagg gaagccgtgc tcgggtacgc ggagacgctc
     2581 tatcccgagt accggaagaa gatcaaggac ac
//
LOCUS       DBNGSH010000020         1757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2208823, whole genome shotgun sequence.
ACCESSION   DBNGSH010000020 DBNGSH010000000
VERSION     DBNGSH010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2208823"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="AC16CY_00465"
     CDS             complement(<1..154)
                     /locus_tag="AC16CY_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR3352274.1"
                     /translation="MRENARFNTVCIHAGQEPDPATGAIVTPIYQTSTYVQEALGRHK
                     GYEYART"
     gene            322..>1757
                     /locus_tag="AC16CY_00470"
     CDS             322..>1757
                     /locus_tag="AC16CY_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR3352275.1"
                     /translation="MTQQLPPGEFPSRAVVALSWLTRTFAGRALIAGIALKIAAWVGS
                     LMGGGAALLTAIDTLGALAFLVGAFVLGFRLYMAARHRLLWRVRRKLVLSYVFIGVVP
                     GLLMALFFGLAGLLFFLNVSSYMLRVRVASLVEAAQFAAQAASPALERAPSSAALAEV
                     LSRRQSDALSRYPLISFAIVPSEKACGGTGTAVIATVTSGPWSHLDPPPVPEWVPCTG
                     FAGLVAYDVGGQTRVAARALTRPRGLGASLLVDIPLGDEIVRTIHDEMGLTITGYSTG
                     NAVAEAAARGGRPSPQGQNLAVATVAQGEPLGWVAVLDYTDWTTGEMDRLTAGFRLGP
                     AAVYRYLSGPSLEALDNRTFGQILLIVLAAVAGMFLVIQAVAMIMGLTLVRSITGSVH
                     ELFAGTERVRRGDFSYKIPIRSRDQLGELAGSFNSMTASIEDLLREKAEKERLEQELL
                     IARSIQMSLLPQGPMRMPGLTLAGHCEPA"
BASE COUNT          248 a          593 c          594 g          322 t
ORIGIN      
        1 gcgtcctcgc gtactcgtag cccttgtgcc ggccgagcgc ttcctgcacg taggttgacg
       61 tctggtagat cggcgtcacg atggcgccgg tcgcagggtc gggctcctgg ccggcgtgga
      121 tgcacacggt attgaatcgg gcgttctcgc gcagcttcac ggcgattgaa gcgtactcta
      181 caccacagct cgcttgtgtt ggtgggctcc ggccgaggcc cgctgaccaa acagcctgtc
      241 ctgcaagcca cgatcgttgg caacatcacc gacacaagcg agttccgggg gtagactggc
      301 taccagctac cagcgactca cgtgacccag cagctaccac caggagaatt cccctctcgc
      361 gccgtcgttg cgctgtcatg gctgacgcgc acgttcgcgg ggcgcgccct gatcgccggc
      421 atcgcgctca agatcgccgc ctgggttggc agcctgatgg gcgggggagc ggcgcttctg
      481 acggccatcg acacgctggg cgccctggcg tttctcgtcg gcgctttcgt cctcggtttt
      541 cgtctctaca tggcggcccg gcaccggctg ctgtggcgcg tgcgcaggaa gctggtgctg
      601 tcttacgttt tcatcggcgt ggtgccgggc ctgctcatgg cgctcttctt cggactggcc
      661 gggctgctct ttttcctcaa cgtgagctcg tacatgctcc gagtacgagt ggcctcgctg
      721 gtcgaggcgg cgcagtttgc cgcgcaggcg gcctcgcccg cgctcgagcg ggcgccctcg
      781 tcggccgcgc tggccgaagt gttgtcgcgc cgccagtcgg acgcgctgtc gcgctacccg
      841 ctgatctcgt tcgcgatcgt gccatccgaa aaggcctgcg gcggcacggg gactgcggtg
      901 atcgccacgg tgacatcggg accctggtcg catctcgatc cgccgccggt ccccgagtgg
      961 gtgccctgca cggggtttgc gggcctcgta gcatacgacg tcggcggcca gaccagggtg
     1021 gcggcccgtg ccctcacgag gccgcgaggc cttggcgcat cgctcctggt tgacatccct
     1081 ctcggcgacg agatcgtccg gacgatccac gacgaaatgg gcctcacaat caccgggtac
     1141 tcgacgggga acgcggtcgc ggaggcggcc gcgagagggg gacgaccgtc gccgcagggc
     1201 cagaaccttg ccgtggccac cgttgcacag ggagaacccc tcggatgggt cgccgtcctc
     1261 gattacaccg actggacgac gggcgagatg gacaggctta ccgctgggtt ccgcctgggg
     1321 cccgcggccg tctaccgcta tctctccggg ccatcgctcg aggcgctgga caaccgcacc
     1381 ttcggtcaaa tcctgctgat cgttctcgct gccgtcgccg gcatgttcct cgtcatccag
     1441 gccgtcgcga tgatcatggg cctcacgctc gtgaggtcga tcaccgggtc ggtgcacgag
     1501 ctatttgccg gcacggagcg ggtccgccgc ggcgacttct cgtacaagat cccgattcgc
     1561 tcccgcgacc agctcggcga gctggcggga tcgttcaact cgatgacggc gagcatcgag
     1621 gacctgctcc gtgagaaggc cgagaaggag cggctggagc aggagctcct gatcgcgcgc
     1681 agcatccaga tgtcgctcct cccgcagggg ccgatgcgca tgccgggcct gacgctggcc
     1741 ggacattgcg agcccgc
//
LOCUS       DBNGSH010000021         3264 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2718085, whole genome shotgun sequence.
ACCESSION   DBNGSH010000021 DBNGSH010000000
VERSION     DBNGSH010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2718085"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="AC16CY_00475"
     CDS             complement(<1..1433)
                     /locus_tag="AC16CY_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352276.1"
                     /translation="MKRSLRSWLWRVPLDQEVEDEIAFHIEMRTRELVAKGLDGKTAR
                     EVVLARIGDTGRLRRTCIDLGRKRDRHMRLTQWFGEVRDDATGAFRQMRASPGFTFVA
                     ALTLALGIGANSAMFALADATFLRPLPFTAPQDRLVMAWERFPNGRLATTTQLDYHDW
                     ADQNTSFEAMAAFVGSGATLAGPDGTLEQVPAQRVTTRFFDVLGVTPIAGRTFLPSDA
                     TTPDVVVLSDDFWRRRFGADPSIVGRSITLDGRPQTVVGIVASRFQLMPASVGFAGGG
                     QPSFWGVFNPPRSGPPFLRGAHFVFVVGRLKPGVPFDAAQRDMTAIGNRNAEKFPDTN
                     KGHEPTLQPLREALVGSEMRLTSALLLGVVGFVLLMCCANVANLLLARTAARARELAI
                     RSALGATRRRVASQLLIESLVLAGVGGIVGVGVGWAILQVAPALVPPGLLPNAVTLSF
                     DGRLVAFCAVATLLVGLLFGLAPAWQFT"
     gene            complement(1526..>3264)
                     /locus_tag="AC16CY_00480"
     CDS             complement(1526..>3264)
                     /locus_tag="AC16CY_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3352277.1"
                     /translation="IAESLAREFPTANKDRGVTVEPMRSSVIGQELRLTSLLFLAVVG
                     FVLLMCCANVANLLLARASGRARELAVRSALGAGRRRIVAQILTESLVLAAIGGLLGL
                     AVGAAILKAAPAVIPAGVLPSAVTLVFDARVVLFCGATAGLVGVLFGLAPAWQASGTS
                     LVQVASSESRTATRGGGRLRSLLATGEIAAAVLLLCGAGLLLRTLVTLDSVDAGYRAE
                     SALTIQVNLGYGLPTSQFPDADALRQFFNAVEREVAAVPGVRSVGWSTTLPLAGSAFG
                     WFGFDIVGKEAAGPADRPLADYQLVTPGYLETLDIPVIAGRGFTAQDSATSVQVCLVS
                     EGLVRRYLKGANPLGMRIAIRPMQLGPSAPIVREIVGVVRQVKTTPADVEETPQVYVP
                     MEQNPWSFVTLAVRPAGGRADALASAIRAAVARVNSGVPTTRVRTLDDVAREATTRPR
                     FRAVMVMTFAALALVLAMVGVFGVIAYSVQQRTREFGVRIALGATSRSVLGLVLGSAA
                     RVISAGVVVGLALAVTFAQAISTFLFGVQPRDPLTFVAVPIVLVLTALAACAAPALRA
                     ARVDPVVAFRNE"
BASE COUNT          618 a         1139 c         1061 g          446 t
ORIGIN      
        1 gtgaactgcc aggcgggcgc caatccgaac aacagcccga ccaggagggt cgcgaccgca
       61 caaaacgcca cgaggcgccc gtcgaaggac agcgtcaccg cattcggaag cagtcccggg
      121 ggcaccagcg cgggcgccac ctgcagaatc gcccacccga caccaacacc cacaattccg
      181 cccacgccgg cgaggacgag gctctcgatg aggagttgcg aggccacgcg ccgccgagtc
      241 gcgccgagcg ccgaacgtat tgccagttcc cgtgcacggg cggcggttcg cgcgaggagc
      301 aggttggcga cgttcgcgca gcacatcagc aggacgaagc ccacgacgcc gagcagcagc
      361 gccgacgtga gacgcatctc ggatccgacg agtgcttctc gcagaggctg caacgtgggc
      421 tcgtggcctt tgttcgtatc cggaaacttc tccgcgttcc gattgccgat cgccgtcata
      481 tcgcgctggg cggcgtcgaa gggcaccccc ggcttgaggc ggccgacaac gaaaacgaaa
      541 tgagcgccgc gcagaaaggg agggcctgac cgcggcgggt tgaagactcc ccagaaagaa
      601 ggctgccctc ccccggcgaa accaacgctc gctggcatga gttgaaaccg gctcgcgacg
      661 atgccgacca cggtttgcgg ccggccgtcg agcgtgatcg aacggccgac gatcgaggga
      721 tcggcgccga accgccgcct ccagaagtca tcgctgagca ccacaacgtc cggcgtcgtc
      781 gcatccgagg gaagaaacgt tcgtccggcg atcggcgtga cgccgaggac gtcgaagaac
      841 cgagtggtga ccctctgagc aggaacctgc tccagtgtgc cgtcaggtcc ggcgagcgtt
      901 gcaccgctgc cgacgaacgc ggccatggcc tcgaacgagg tgttctgatc ggcccagtcg
      961 tggtagtcga gctgcgtcgt ggtggcaaga cgcccgttcg ggaatcgctc ccacgccatc
     1021 acgagccgat cctgcggcgc cgtgaatggc agcggcctca gaaacgtcgc gtcggcaagc
     1081 gcaaacatcg cgctgttggc gccgatgccg agcgccagag tcagcgcggc cacgaaggtg
     1141 aagccgggcg acgccctcat ctgacggaac gcgccggtgg catcgtctct gacctcgccg
     1201 aaccactgtg tgaggcgcat gtggcgatct cgctttcgtc caaggtcgat gcaggtccgc
     1261 ctgagtcgac cagtgtcccc gatccgcgcg agcaccacct cgcgcgccgt cttgccgtcc
     1321 agccccttcg ccacgagctc gcgcgtccgc atctcgatgt ggaacgcgat ctcgtcttca
     1381 acttcctgat cgagcggcac gcgccagagc cacgagcgca gcgaccgctt catctcgaga
     1441 tcacacgacg aaccacggag acacggagaa gaggtggcct tcttgctccg tgcctctgtg
     1501 cctctgtggt ttgtgataat gcgcgctact cattgcgaaa cgccaccacc gggtcgacgc
     1561 gagcggcacg cagggctgga gcggcgcagg ctgcgagcgc cgtgagcacc agtacgatcg
     1621 gcacggccac gaaggtcagc ggatcccgcg gctgcacgcc gaacagaaac gtcgagatgg
     1681 cctgggcgaa cgtcaccgcc agcgcgagcc cgacaacgac gcccgcactg ataacacgcg
     1741 ccgcgctgcc gaggacgagc ccgagcacgc ttcgagaggt ggcgccgagt gcgatacgga
     1801 cgccgaactc gcgcgtacgt tgctggacgg aataggcaat cacaccgaag acgccgacca
     1861 tggcgagcac gagcgcgagg gccgcaaacg tcatcaccat cacggcgcgg aaccgtgggc
     1921 gcgtcgtcgc ctctcgcgcc acgtcatcga gagtcctgac gcgcgtcgtg gggacaccag
     1981 aattcacgcg cgccactgcg gccctgatcg cggacgcgag cgcgtcggcg cgtccgcccg
     2041 ccggccgcac tgccagcgtc acgaagctcc acgggttctg ctccatcggg acgtagacct
     2101 gcggggtctc ctcgacatcg gcaggcgtag tctttacctg acgcacgacg ccgacgatct
     2161 ccctcacgat cggcgccgac ggcccgagct gcatcggcct gatcgcgatg cgcatcccca
     2221 gtggattcgc acccttcagg taccgccgga cgaggccttc actcaccaga cacacctgga
     2281 cgctcgtcgc gctgtcctgt gctgtgaagc cgcgtcccgc gatgacagga atgtcgagcg
     2341 tttccagata gccgggcgtg acgagctggt aatcggccag tggacgatcc gccgggccgg
     2401 ccgcctcctt tccaacgatg tcgaagccga accagccgaa ggcgctgccc gcgagcggca
     2461 gcgtcgtgga ccagccgaca cttctcacgc caggcacggc cgccacttct cgctcgacgg
     2521 cgttgaaaaa ctgccgaagc gcgtcagcgt cgggaaactg ggacgtcggc agtccatacc
     2581 ccaggttgac ctgtatcgtg agtgcgcttt cggcccggta tccggcatcc acgctgtcga
     2641 gggtgacgag cgtccgcaga agcaaaccgg caccgcacaa cagcagcacg gcggccgcga
     2701 tctccccggt cgcgagcagg ctgcggagtc gtcctccacc cctggtcgcc gtacggctct
     2761 cggaggacgc tacctggacg agcgacgttc cgctcgcctg ccacgccggc gccagaccaa
     2821 agagaacgcc gacaagaccc gcggtcgcac cgcagaacag cacgacgcgg gcatcgaaga
     2881 cgagcgtcac ggccgacggg agcacgccgg ctggaatgac cgcgggcgcc gccttcagga
     2941 tcgcggcgcc gacggcgagg ccgagaagtc caccgatcgc cgccagcacg aggctttccg
     3001 tcagaatctg cgccacgata cggcggcgcc cggcgccgag cgccgacctc acggcgagct
     3061 cgcgcgcgcg tccgctcgca cgggccagaa gcaggttggc gacgtttgca cagcacatca
     3121 gcagcacgaa cccgacgacg gcgagaaaca gcagcgacgt gaggcgcaac tcctgcccga
     3181 tgacgctgct tcgcatcggc tccacggtga cgcctcgatc cttgttggcc gtcggaaact
     3241 cgcgggcgag cgactcggcg atcg
//
LOCUS       DBNGSH010000022         1844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2888050, whole genome shotgun sequence.
ACCESSION   DBNGSH010000022 DBNGSH010000000
VERSION     DBNGSH010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2888050"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="AC16CY_00485"
     CDS             <1..770
                     /locus_tag="AC16CY_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352278.1"
                     /translation="TEYTFTAPVHPQLPGGGGYPVTSLNPRTNFSAANNYTTFASDYG
                     EMYQYWHGFDVNVTSRMRNGLVVQGGTSTGRGVRDNCEITAKVPELLLSAGTWAQAGA
                     CHVDEPWLTQVRGLATYTIPRADVQLSTSFQFKPGTLGIGGNDAATNGQSLNANYVVT
                     SAVAGIPLLNNQQTVNLLVPGQLYGEWVNQIDVRVGKIFRIGRTRTLVAIDMYNLLNS
                     NPGLTYQQGFTGAGATWLNPSSLLMPRFFRFNATVDF"
     gene            854..>1844
                     /locus_tag="AC16CY_00490"
     CDS             854..>1844
                     /locus_tag="AC16CY_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3352279.1"
                     /translation="MSTRSTVAALAACWLAGCGGSAPSSPAPSSRPDSEPYFAERAAA
                     AGIDFTHFNGMSGEFYYPEIMAPGVGLLDYDNDGDLDLYLVQGQMLGGKGIDRATFAP
                     RGPLRDRLFRNDLTIDSDGRRTLRFTDVTDQAGIDVRTYGMGVAAGDIDNDGFPDLYR
                     TGLTEGVLLHNNGNGTFTDITAKSGTANKGGWGVSAAFADIDRDGWLDLYVGNYLFYS
                     IEGDIDCLSVTGQHDYCPPNSYRPQPDKLYRNRGNLSFADVTRTALEGGAYGPALGVS
                     TADFNNDGWIDIYVGNDGMPNQLWINQKNGAFKDMAFINGAAVNGAGNAEASMG"
BASE COUNT          345 a          608 c          590 g          301 t
ORIGIN      
        1 acacggagta cacgttcacg gcgccggtgc acccgcagct tccaggcgga ggcggatacc
       61 cggtgacgtc gctcaatcct cggacgaact tctccgccgc caacaactac acgacgtttg
      121 ccagcgacta cggcgagatg taccagtact ggcacggctt cgacgtcaac gtgacctcac
      181 ggatgcggaa cgggctggtg gtccagggtg gtaccagcac gggccgcggc gtgcgcgaca
      241 attgcgagat cacggccaaa gtgcccgaac tgctgctgag cgccgggacg tgggcgcagg
      301 ctggggcctg ccacgtcgac gagccgtggc tgacccaggt gcgcggcctg gcgacctaca
      361 ccattccgag agcggacgtt cagctgagca ccagcttcca gttcaagcct ggcacgctcg
      421 gcatcggcgg caacgacgcc gcaaccaacg gccagtcgct caacgccaac tacgtcgtca
      481 cgagcgcagt tgcgggaatc ccgctgctca acaatcagca gacggtgaac ctcctcgtcc
      541 cgggtcagct gtacggcgaa tgggtaaacc agatcgacgt ccgcgtcggg aagatcttcc
      601 gaatcggccg gacgcgtacg ctcgtggcga ttgacatgta caacctgctc aactcgaacc
      661 ccggtctgac gtatcagcag ggattcaccg gagcgggcgc aacgtggctc aatccgagct
      721 ctctgctgat gccgcgcttc ttccggttca acgcgacggt ggacttctaa agagccgccg
      781 gctggcagct ttcagtcaag aggggcgggc ccgtgcttcg tgggcccgcc cttttcctgt
      841 acgcttcctt cggatgtcga cgcgctcgac cgtggccgcg ctggcggctt gctggcttgc
      901 cggatgcggc ggttcggcgc cgtcctcgcc ggcaccgtcg tcgcggccgg acagtgagcc
      961 ctacttcgcc gagcgcgcgg ccgccgccgg gatcgacttc acgcacttca acgggatgtc
     1021 tggcgagttc tactacccgg agatcatggc gcccggagtc ggcctgctcg actacgacaa
     1081 cgacggcgac ctggacctgt acctcgtgca gggacagatg ctcggtggga aggggattga
     1141 tcgcgcgaca tttgccccgc gcggcccgct gagggatcgt ttgtttcgca acgacttgac
     1201 gatcgattcc gacggccgca ggacgctgcg cttcacggac gtgaccgacc aggccgggat
     1261 cgacgtccgc acctatggca tgggcgtggc cgcgggcgac atcgacaacg acggctttcc
     1321 ggacctttat cgtacgggac tgacggaagg cgtgctcctg cacaacaacg gcaacggcac
     1381 cttcactgac atcaccgcga agagcgggac ggcaaacaag ggcggctggg gcgtgtcggc
     1441 ggccttcgcc gacatcgatc gcgacggatg gctcgatctc tacgtcggca actacctctt
     1501 ctacagcatc gaaggcgaca tcgactgcct ctcggtgacg gggcagcacg actactgccc
     1561 gccgaacagt taccgcccgc agcccgacaa gctctatcgc aaccgcggga acctgagctt
     1621 cgccgacgtg acgcggacgg cgctcgaggg tggtgcgtac ggcccggccc tcggcgtctc
     1681 gaccgccgac ttcaacaacg acggatggat cgacatttac gtcggcaacg acgggatgcc
     1741 gaatcagctc tggatcaatc agaagaacgg cgccttcaag gacatggcgt tcatcaacgg
     1801 cgccgccgtc aacggcgcgg gcaacgccga agccagcatg ggga
//
LOCUS       DBNGSH010000023         5900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1699427, whole genome shotgun sequence.
ACCESSION   DBNGSH010000023 DBNGSH010000000
VERSION     DBNGSH010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1699427"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="upp"
                     /locus_tag="AC16CY_00495"
     CDS             complement(<1..557)
                     /gene="upp"
                     /locus_tag="AC16CY_00495"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR3352280.1"
                     /translation="MELRNVNTAPPAFRRAATRISLLMAAEALRSLPTVRATVTTPLG
                     PAEGRVVQADVVVVPVLRAGLSMLEPVLQLIPSARVGHIGLRRDEETAVAERYYSKLP
                     PGIGDSIVLMIDPMLATGGSSSAAIDLVKAAGARSISLACVISAPEGVAQLLARHPDV
                     SIFTPVVDRELNARKFIVPGLGDFG"
     gene            628..2028
                     /locus_tag="AC16CY_00500"
     CDS             628..2028
                     /locus_tag="AC16CY_00500"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3352281.1"
                     /translation="MAPRLSVAAALAVAALVVVSAQNAQREVALVVTGGTVVTVDAAG
                     RIVGNGAVAIDGADIVAVDSVEAIARQFRGRETIDASGQVVLPGLVNTHTHAAMVLYR
                     GLADDLPLAEWLNNYIFPAEARTVSPEFVRAGTKLAALEMIESGTTTFADMYYFEEEV
                     ARETRAAGLRGVLGQTIIQFPVADAKTPADGLARAEEFIKAFRSDALITPAVAPHSMY
                     TLDGPTLRAARELSKRYNVPTLIHLAETTEDARVAKERRAASPVAYLESLGFLSSGVV
                     AAHGVWVSDDDIKILKTHDVGVAHNPESNMKLASGTAPVPAYLRAGVSVGLGTDGAAS
                     NNDLDMFDAMRVAALLHKHTTVDPSVLTAKTVLELATIRGAHALGMGGRIGSLEPGKR
                     ADLVTVSLARARLTPVYDPISHLVYAAHGDDVQNSIVNGRVLMRNRKVLTMNVAAVLD
                     DARSWGEKVKAAVKPR"
     gene            complement(2063..2569)
                     /gene="hpt"
                     /locus_tag="AC16CY_00505"
     CDS             complement(2063..2569)
                     /gene="hpt"
                     /locus_tag="AC16CY_00505"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006556382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR3352282.1"
                     /translation="MQTLITEAQLRERVRALALDISRDHPDGVHLVSVLKGAFMFLAD
                     LVRAMPGEVTLDFMALSSYGNRQTTSGEVRLLKDLDAGLEDRSVVIVEDIVDTGLTLA
                     YLQEILRARTPRSLRTACLLNKPSRRQTSVVVDYVGFTIDDEFVVGYGLDSRERYRNL
                     PYIAVVNE"
     gene            2614..3249
                     /locus_tag="AC16CY_00510"
     CDS             2614..3249
                     /locus_tag="AC16CY_00510"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR3352283.1"
                     /translation="MKRFQMITEADARALDYGSTVVLVAGGHVTPLALDTLKARRITV
                     VRDGIDPDAADLAPASAIRTVAIAGDHTSLALKSAIVRHLRGKGLAAHDLGTNATEPV
                     DYPDTAAAAALAVARGEADAAIVIDGAGLGSTIAANKVPGARAAMCSNTTLARYAREH
                     NGANVLALGSALVNGDEAIAIVDTFLGTPMREARYIRRLAKIRDLERHPRR"
     gene            3453..4124
                     /gene="deoC"
                     /locus_tag="AC16CY_00515"
     CDS             3453..4124
                     /gene="deoC"
                     /locus_tag="AC16CY_00515"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020278358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR3352284.1"
                     /translation="MIDHTLLKPDATRKEIEALCREASEYGFASVCVNPTWVALCASL
                     LRGSQVAVCSVVGFPLGATTPDTKQYETRRAIFDGAREIDMVVNVGALKSGDLPLVGR
                     DIEAVTSACREGGALSKVIIEAALLTDDEKVAACTLAKAAGADYVKTSTGFGPGGATA
                     ADVALMRRTVGEELGVKAAGGVRDLDALKAMVAAGATRIGASAGVRIVQQSRGGAPGR
                     STSSY"
     gene            4134..4730
                     /locus_tag="AC16CY_00520"
     CDS             4134..4730
                     /locus_tag="AC16CY_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3352285.1"
                     /translation="MARCRPAVLLIAMWSVTLHPGGADAQALNPGPPGPYVVDVRGAM
                     SGVPTDPVYFPSLSAGEAVPGRGFGATLGGHVYAFRIGAARLGFGADVMIARGATDDV
                     STTLRTIAPQVSFNFGTSDGWSYLSGGVGTARVTVDPGSSQSVRATSLGGGARWFLGP
                     HVGVGFDARFHWLAKGGEAGSETPASMAFAVAVGVSFK"
     gene            complement(4903..4977)
                     /locus_tag="AC16CY_00525"
     tRNA            complement(4903..4977)
                     /locus_tag="AC16CY_00525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4943..4945),aa:Gly,seq:ccc)
     gene            complement(5005..>5900)
                     /locus_tag="AC16CY_00530"
     CDS             complement(5005..>5900)
                     /locus_tag="AC16CY_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR3352286.1"
                     /translation="YVDKVFSFIEPAIIKPFNVVLDAGSGMAGLVAPMLFDRLPCRTT
                     RLCFDVDGTFPNHEANPLIEENRRDITAEVVRQGADIGIAWDGDADRCFFIDGSGEFI
                     SGDFVTALLAEAFLLKHPGATVIYDLRASHAVRDTVAAHKGKALMNRVGHAFIKQRMR
                     QEDGIFAGEVTGHYYFRDFYYADNGFIPALLILELMSKKNMPLRDLLRPYRERYFISG
                     EINTTLRSVEDAPKKLAAIEARYRDATLSRMDGLSVDYADWHFNIRASNTEPLLRLNL
                     EAKTPEMMERKRDEVLELIRA"
BASE COUNT          960 a         1908 c         2070 g          962 t
ORIGIN      
        1 tcgccgaagt cgccgagccc gggaacgatg aacttgcgcg cgttgagctc gcgatcgacg
       61 acgggcgtga aaatggagac gtcggggtgc cgcgcgagca gttgggcgac tccctcgggc
      121 gccgagatga cgcacgccag agaaatggac ctggcgccgg ccgccttgac caaatcgatc
      181 gcggccgagg agctgccgcc ggtggcgagc atagggtcga tcatcagcac gatgctgtca
      241 ccgattccgg ggggcagctt ggaatagtac cgctcggcca ccgctgtctc ctcgtcccgg
      301 cgcagcccga tgtggccgac gcgagccgac ggaatcagct ggagcaccgg ctccagcatg
      361 ctgagtcccg ccctcagcac aggaacgacg acaacatcgg cctgcacgac gcgcccttca
      421 gccggcccga ggggtgtcgt gaccgtggct cgcactgtcg gaaggctccg gagcgcctcg
      481 gcagccatca gcaaactgat gcgcgtggcg gccctcctga aggcgggcgg ggccgtattg
      541 acgttccgca gctccatcag ggcgtcgtgc acgagcgggt gatcaatcgt gtgaaggggc
      601 accttgaaat agtactaaaa tccccctatg gccccccggc tttccgtggc tgccgccctc
      661 gcggtggctg cgctggtcgt cgtctcggct cagaacgcgc agcgggaggt cgccctcgtc
      721 gtgaccggcg gtacggtcgt gacggtggac gcggccggcc gcatcgtggg caacggcgcc
      781 gtggcgatcg acggcgccga catcgtggcc gtcgattcgg tcgaggccat cgcgcggcag
      841 ttccgcggcc gcgagacgat cgatgcgtcg gggcaggtcg tgctgccggg cctcgtcaac
      901 acgcataccc acgcggccat ggtgctctat cgcggcctgg ccgacgacct gccgctggcc
      961 gagtggctga acaactacat cttccccgcg gaggcgcgga cggtctcgcc cgagtttgta
     1021 cgcgccggca cgaagctggc cgcgctggag atgatcgagt ccggcacgac gaccttcgcc
     1081 gacatgtact acttcgaaga ggaggtcgcg agggaaacgc gggcggcagg gctgcgaggc
     1141 gtcctcggcc agacgatcat ccagttcccg gtggccgacg ccaagacgcc ggccgatggc
     1201 ctcgcgcggg cagaagagtt catcaaggcc ttcaggagcg acgcgctgat cacgccggca
     1261 gtggcgcctc attcgatgta cacgctcgac ggccccacgc ttcgagccgc gcgcgagctc
     1321 tcgaagcgtt ataacgtccc gacgctcatt catctcgcgg aaacgaccga agacgcgagg
     1381 gtcgcgaaag agcggcgcgc ggcgtcaccc gtggcgtacc tggagagcct ggggttcctg
     1441 agctcgggcg tcgtggccgc gcatggcgtc tgggtgtcgg acgacgacat caagatattg
     1501 aagacccatg acgtcggagt ggcgcacaac cccgagagca acatgaagct ggcaagcggc
     1561 acggcgccgg tccctgccta tctccgcgcc ggcgtgtcgg tcggcctggg caccgacggc
     1621 gcggccagca acaacgacct cgacatgttc gacgccatgc gcgtggccgc gctgctgcac
     1681 aagcacacga cggtggaccc gtccgtgctc acggcgaaaa ccgtgctcga gctcgcgacc
     1741 attcgcggcg cgcacgcgct cggcatgggc ggacgcatcg gctcgctcga accgggcaag
     1801 cgggccgatc tggtgacggt gtcgctggcc cgcgcccgcc tcacgcccgt ctacgacccg
     1861 atctcgcacc tggtgtacgc cgcgcacggc gacgacgtgc agaacagcat cgtcaatggc
     1921 cgcgtcctga tgcggaaccg caaggtgctg acgatgaacg tagccgccgt cctggacgac
     1981 gcgcggtcgt ggggagaaaa ggtcaaggcg gcggtgaagc cgcgatgaag ccgccgggcg
     2041 gcagcaggcg agaaaaccgc gttcactcgt tgacgacggc gatgtagggc aggttgcgat
     2101 accgttctct cgaatcgagc ccatacccca cgacgaactc gtcatcaatc gtgaagccga
     2161 catagtccac gacgacgctg gtctgccgcc gcgacggttt gttgagcagg caggccgttc
     2221 tcaagcttcg gggcgtgcgc gcgcggagga tctcctgcag gtaggcgagc gtcagcccgg
     2281 tatcgacgat gtcctcgacg atgaccaccg agcgatcctc gaggccggca tcgagatcct
     2341 tgaggagcct cacttctccc gacgtggtct ggcgattgcc gtagctcgaa agcgccatga
     2401 aatcgagcgt gacctcgccc ggcatggcgc gcacgagatc ggcaaggaac atgaaggcgc
     2461 ccttcaggac cgataccagg tgaacgccgt ccggatggtc gcgtgagatg tcgagcgcga
     2521 gcgcgcggac tcgctcgcgg agttgtgcct cggtgatcag cgtctgcatg ccttgagcgg
     2581 tcgtgcgcgc aagggtaaca tagcgccccg aacgtgaagc gttttcagat gatcaccgag
     2641 gcggacgcgc gcgcgctcga ctacggcagc acggtcgttc tcgtcgccgg tggccacgtc
     2701 acgcccctcg cgctcgacac gctcaaggcg cgccgcatca cagtcgttcg cgacggcatc
     2761 gatcccgacg ccgcggacct ggctccggcc tccgccatcc gtacggtggc gattgcgggc
     2821 gatcacacgt cgctggcgct gaaatcggcg atcgtccggc acctgcgcgg gaagggcctc
     2881 gcggcgcacg acctcggcac gaacgccacc gagcccgtcg attatcccga caccgccgcg
     2941 gcggcggctc tggcggtcgc acgcggcgag gccgacgcgg cgatcgtgat cgacggcgcc
     3001 ggtctcgggt cgaccatagc agccaacaag gtgccgggcg cccgcgcggc gatgtgttcg
     3061 aacacgacgc tcgcccgata tgcccgtgag cacaacggcg ccaatgtcct ggcgctgggc
     3121 tcggcgctgg tgaacgggga cgaggcgatc gccatcgtgg acacctttct cgggactccc
     3181 atgcgcgagg cgcgctacat caggcggctg gccaagattc gggacctcga acgccatccg
     3241 cggcgctgat gccacccgtc gatctcgagc ggctcatcca gatcatcgtc gaggagctga
     3301 cgtcctcggg cgccgtgtcg gctccgttgc ggtgcgcgtg tcactcggtg atctacgagt
     3361 gctgccccag ccgggtggct cctgtggtcg aggccggggc cacgcgtctc ggcctccacg
     3421 ccgccggtgg cggcgcaggc ggcgtggcgg gaatgatcga ccacacgctg ctgaagcccg
     3481 acgcgacgag gaaggagatc gaggcgctgt gccgcgaagc gtcagagtat ggctttgcca
     3541 gcgtctgcgt gaaccccacg tgggtggcgc tgtgcgcgag cctgcttcgc gggagccagg
     3601 tcgccgtctg ctcggtcgtg gggtttccgc tcggcgctac gacacctgac acgaagcagt
     3661 acgagacgcg gcgggcgatc ttcgacgggg ctcgcgagat cgacatggtc gtcaacgtcg
     3721 gcgccctcaa gtccggcgac ctgcccctgg tcgggcggga catcgaggcg gtgacatcgg
     3781 cgtgccggga gggcggggcg ctgagcaagg tcatcatcga ggcggcgctg ctgaccgacg
     3841 acgagaaggt cgccgcgtgc acgctggcga aggccgccgg cgccgattac gtgaagacct
     3901 cgacgggctt cggcccgggt ggcgcgacgg cggcggacgt ggcgctgatg cgccgaaccg
     3961 tcggcgagga gctcggcgtg aaagcggccg gcggcgtacg ggacctggat gcgctgaagg
     4021 cgatggtggc ggccggcgcc acgcgcatcg gcgccagcgc gggcgtccgc attgtccagc
     4081 agtcccgcgg cggcgcgccg ggcaggtcaa cgtcttccta ctgacacgct gcgatggcac
     4141 gttgccgccc ggccgtcctg ctgatcgcga tgtggtccgt cacgcttcat ccgggcggtg
     4201 cagatgcgca ggcgctcaat cccggcccgc cgggcccgta cgtggtggac gtgcgcgggg
     4261 cgatgagcgg cgtgccgacc gacccggtgt actttccgtc gctgtcggcg ggggaggcgg
     4321 tacccgggcg tggcttcggg gcaaccctcg gcggccatgt ctacgcgttt cggatcggag
     4381 ctgcccggct cgggttcggt gcggacgtca tgatcgcacg cggcgcgacc gacgacgtca
     4441 gcaccaccct cagaacgatt gcgccgcagg tctcgttcaa cttcggtacg tcggacggct
     4501 ggagttatct gagcggcggc gtggggactg cgcgcgtgac cgtggatccc ggttcgtccc
     4561 agagcgtccg cgcgacgagt ctcggcggcg gcgcgcggtg gtttctcggt cctcacgtcg
     4621 gcgtcgggtt cgacgcccgc tttcactggc tggccaaggg cggagaggcg ggatcggaga
     4681 cgccggcgtc aatggcgttt gcggttgcgg tgggtgtgtc gttcaagtag tgtccgcctt
     4741 caggcggacc aagcgcggat cacggtccgg ctgaagccgg acactacgtc ctcatgagcg
     4801 aaggttggct tgccgtccga agctcgttcg ggcagtgagt aggcccacct tcgcgcattg
     4861 cgctacggtg ggcatccttc gcctgcgttc atgagcgaag gatggagcgg gagacgggga
     4921 tcgaacccgc gacatcgagc ttgggaagct cgcgttctac cgctgaacta ctcccgcttc
     4981 ggtccactca attgtaagtc aaaactacgc tcgaatgagt tccagcactt cgtcgcgctt
     5041 ccgttccatc atctccggcg tctttgcctc gaggttcagt cgcagcagcg gttccgtatt
     5101 ggacgcccgg atgttgaagt gccagtcggc gtagtccact gacaggccgt ccatccggga
     5161 gagggtggcg tcacggtagc gcgcctcgat cgccgccagc ttcttcggcg cgtcttcgac
     5221 gcttcgaaga gtggtgttga tctcacccga gatgaagtag cgctcgcggt acgggcggag
     5281 cagatcccgc agcggcatgt tcttcttcga catgagttcc aggatgagca gcgcgggtat
     5341 gaaaccgttg tcggcgtaat agaagtcgcg gaagtagtag tgtccagtga cttcgcccgc
     5401 gaaaatgccg tcttcctgcc gcatccgctg cttgatgaac gcatggccga ctcggttcat
     5461 cagcgccttt cccttgtgtg cggcgacggt atcgcggacg gcatgactcg cgcgaaggtc
     5521 gtagatgacg gtggcgcccg ggtgcttcag caggaaggcc tcggcgagca gcgccgtcac
     5581 gaagtcgccc gagatgaact cgccgctgcc gtcgatgaag aagcagcgat cggcgtcgcc
     5641 gtcccaggcg atgccgatgt cggcgccctg gcgcacgact tcggcggtga tgtcccggcg
     5701 gttctcctcg atcagcgggt tcgcctcgtg attgggaaag gtcccgtcca cgtcgaagca
     5761 caggcgcgtc gtccggcagg ggaggcggtc gaagagcatc ggcgcgacga gccccgccat
     5821 gccgcttccc gcgtcgagca ccacgttgaa cggtttgatg atggccggct cgatgaacga
     5881 gaacactttg tccacgtact
//
LOCUS       DBNGSH010000024         3436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1189985, whole genome shotgun sequence.
ACCESSION   DBNGSH010000024 DBNGSH010000000
VERSION     DBNGSH010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1189985"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3436
                     /locus_tag="AC16CY_00535"
     CDS             <1..>3436
                     /locus_tag="AC16CY_00535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HIR3352287.1"
                     /translation="GTGLARGYLGRPSLTAERFVPSTLSEHGGARLYKTGDLVRYLES
                     GAIEFLGRLDYQLKIRGFRVELGEIETALTSHPMVRGSVVVAQAGPTAKRLIAYLVAE
                     SDRTPSTSQLREYLAQRLPDYMVPAVFMTLDAFPLTANGKVDRRALPAPDQSRPELDS
                     QFEAPRPGSEAQLAAIWSELLGVDRIGRHDNFFELGGHSLIGTQVVSRVRDIARVDVP
                     LRHLFERPTVAGLAEWLDKAAADQQERRDPGLSPQAGDEPPVMSFTQERLWLLEQLIL
                     GTPVYNIPAVVRLKGALNVTALGQAIDEVRRRHEVLRTNFHTVDGQPVPVASPASARI
                     VPIADLSDLAHDANATAVRLLIRAEALRPFDLAGDPLIRISLVRLGASEHVMLATMHH
                     VVSDGWSIGILMRELASLYRAFCSGTRSPLAEPAIQYRDFAHWQRRWLQGRTLDEQLS
                     YWTNALSGARTSIELPIDRPRTSPPSYRGSTESRLLQPELVTALHAFGAERGLTPFMV
                     ILAALDILLARMSRQSDLVVGTVISGRTRTEIEPLIGCFMNFLPLRTTVARDASGETV
                     LQQVRTTVLDADAHQECPFQKIVEAARPERSSSQNPLYNVSFQFDNYPQGHSFTDTLE
                     ASFETPHRQISQLDLRFVASETRDGTVLSCEYATDLFDPETVRGLLEALDAILGKLIQ
                     EPAASVGEFPVPESLLRRAQPETVAIAATFTAEPVEESIAFWTDHLDVPCTTQFAPFG
                     QVFQQLLAPDSLLAANRRGLNVVLVRFEDWQSSNLQREVEELGRAVTMAAERIATPWL
                     VCICPSSPATLSDPAQQALHERMEVLLASRLQDTNGVALFRTGDLARAYPVPDYYDRH
                     GDEIGRVPYTRAFFAALGTAIVRHIAAIRRSPYKVIVLDCDQTLWRGVVGEDGVDGIA
                     IGSAHKALQEFMVAQHDAGKLICLCSKNQESDVLEVFDRRDDMVLRRDHLVSWRINWQ
                     AKSQNIRSLADELQLGLDSFVFLDDSPVECAEVRANCPEVLSLQIPTAAGQMPAFLRS
                     VWAFDHVRTTSEDRQRTRLYQQNAERERLREKSLTFADFMADLDLQVTIDPMSPPQLS
                     RVSQLTQRTNQFNVHKVGRSEVELDALSREANTHCLTVGVSDRFGDYGLVGVAICES"
BASE COUNT          582 a         1141 c         1122 g          591 t
ORIGIN      
        1 gggaccgggc tggcgcgcgg gtacctcggc cgtccatcgc tgacggcaga gagattcgtt
       61 ccgagcacgc tcagcgaaca cggaggggct cgtctctaca agacgggcga tctggttcgc
      121 tatctggaga gcggcgcgat cgagttcctg ggccgtctcg actaccagct gaagatccgc
      181 ggcttccgcg tcgagctcgg cgagatcgaa acggcgttga caagccatcc gatggttcgc
      241 ggcagcgtcg tggttgcgca ggccggcccg acggccaagc gcctgatcgc ctacctggtg
      301 gccgaatcgg atcgcactcc ttcgaccagc cagttgcggg agtacctggc gcagcgcctg
      361 cccgactaca tggttccagc ggtgttcatg acgctggacg ccttcccgct gacggccaac
      421 ggaaaggtgg accggcgcgc gctgccggca cccgaccagt ctcgtcccga gctggacagc
      481 cagttcgagg caccgcgccc agggagcgag gcgcagctgg cggcgatctg gtccgagctg
      541 ctgggcgtcg atcggatcgg ccgtcacgac aacttcttcg aactcggcgg ccactcgctc
      601 atcggcaccc aggtcgtgtc cagggtgagg gacatcgccc gcgttgacgt gccgctgagg
      661 catctgttcg agcggccgac ggttgccggc ctcgcggagt ggctcgacaa ggctgcggcg
      721 gaccagcagg aacgccgcga tcccgggctg tcaccccagg ccggcgatga gccgccggtc
      781 atgtccttca cacaggagcg gctgtggctg ctcgagcaac tgatcctggg tacaccggtc
      841 tacaacatcc cggcggtcgt tcgcttgaaa ggcgccctca atgtcacggc cctcggccag
      901 gcgatcgacg aagtcaggcg acggcacgag gtgttgcgca cgaacttcca caccgtagac
      961 ggccagccgg ttccagtcgc cagccccgcg tcggcccgca tcgtgccgat cgcggatttg
     1021 agtgatctgg cacacgacgc caatgcgacg gccgtgcgac tgctgatcag agcggaggcg
     1081 ctccgcccgt tcgatctggc cggcgacccg ctcattcgga tctccctcgt ccggctgggc
     1141 gcatccgagc acgtgatgct cgccaccatg caccatgtcg tctcggacgg atggtccatt
     1201 ggcatcctga tgcgggagct ggcctcgctg taccgtgcgt tctgctcggg cacgcgatcc
     1261 ccgctggcgg agccggccat ccagtaccgg gacttcgcgc actggcagag gcgctggctg
     1321 cagggtcgga cgctggacga gcagctctcg tactggacca acgccttgtc cggcgcccgc
     1381 acgtcgatcg agctgccgat cgatcgcccg cgcacgtcgc cgccaagcta tcgcggctcg
     1441 acggaaagcc ggttgctgca accggagctc gtcacggccc tccacgcctt cggcgccgaa
     1501 cgcggcctca cgcccttcat ggtcatcctg gctgcgttgg acatcctgct cgcacggatg
     1561 tctcgacaat ccgatctcgt cgtcgggacg gtgatctcgg gacggacgag gacagagatc
     1621 gagccgctca taggctgctt catgaacttc ctgccgcttc gcacgaccgt cgcacgcgac
     1681 gcgagcggcg agaccgtcct tcagcaggtg cggacaactg tgctggacgc cgacgcgcac
     1741 caggagtgtc ccttccagaa gatcgtcgaa gccgccaggc cggagcgaag cagcagccag
     1801 aatccgctgt acaacgtttc tttccagttc gacaactatc cgcaaggcca ctcgttcact
     1861 gacacgctgg aggcgtcctt cgagacaccc catcggcaga tctcccagct cgatctgcgc
     1921 ttcgtggcgt cagagacgcg ggacgggacg gtgctctcgt gcgagtacgc cacggatctg
     1981 ttcgaccccg agacggtacg cgggctgctc gaagccctgg acgcgattct ggggaagctc
     2041 attcaggagc cggcggccag cgtcggggag ttccccgttc ccgagtcgct cttgcgtcgc
     2101 gcccagccgg aaaccgttgc cattgccgcg accttcaccg cggagccggt cgaagagtcg
     2161 atcgcgttct ggacggacca cctggacgta ccgtgcacca cgcagttcgc tcccttcggt
     2221 caggtctttc aacagttgct ggcacccgac agcctgctgg ccgcgaatcg gcgggggctg
     2281 aacgtggtac tggttcgctt cgaagactgg cagtcgtcga atctgcagcg ggaagtcgaa
     2341 gagctcggcc gcgccgtgac gatggccgcc gaacggatcg cgaccccctg gctcgtgtgc
     2401 atctgtcctt cgtctccggc gactctgtcg gatccggcgc aacaggccct gcacgagcgc
     2461 atggaagttc tgctggcctc gcgcctgcag gacacgaacg gggtcgcgtt gttcagaaca
     2521 ggggacctcg cgcgcgccta tccagtgccg gattactacg accgtcacgg agacgagatc
     2581 gggcgcgttc cctacacacg tgcgttcttc gcggcgctcg ggaccgccat cgtcaggcac
     2641 atcgccgcga tcaggcgcag cccgtacaaa gtcatcgtcc tcgactgtga ccagacactg
     2701 tggagaggcg tcgttggcga agacggcgtc gatggcatcg ccatcggctc cgcgcacaag
     2761 gccctgcagg agttcatggt cgcgcagcac gatgctggca agctgatctg tctctgcagc
     2821 aagaaccagg aatcggacgt cctggaggta ttcgaccgcc gggacgacat ggtgctgcga
     2881 cgtgaccatc tcgtgtcctg gcgtatcaac tggcaagcga agtcacagaa catccgatcg
     2941 ctggccgacg aactgcagct gggcctcgac agcttcgtgt tcctggacga cagtccggtc
     3001 gagtgcgccg aggtccgggc caactgcccc gaggttctgt cgctgcagat tccgacggcg
     3061 gccgggcaga tgcctgcgtt tctccggagc gtctgggcgt tcgaccacgt gcggaccacg
     3121 tccgaggacc gccagagaac gcgactctat cagcagaacg ccgagcggga gcggctgcgg
     3181 gagaagtcgc tgaccttcgc ggatttcatg gccgacctcg atctgcaggt gacgatcgac
     3241 ccgatgtcgc cgccgcagct gtcgcgagtg tcgcagctga cccagcgaac gaatcagttc
     3301 aacgtccaca aggtgggccg ctccgaagtc gagctggatg cgttgtcacg cgaggcgaac
     3361 acgcactgtc tcacagtggg cgtgtcggac cggttcggag actacggact ggtcggcgtc
     3421 gccatctgcg agtcgg
//
LOCUS       DBNGSH010000025         2066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_850466, whole genome shotgun sequence.
ACCESSION   DBNGSH010000025 DBNGSH010000000
VERSION     DBNGSH010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_850466"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="AC16CY_00540"
     CDS             <1..1466
                     /locus_tag="AC16CY_00540"
                     /inference="COORDINATES: protein motif:HMM:NF019259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3352288.1"
                     /translation="GVAFVQRTEARDEATLRPRMRSRGTQPAINSVTISGPFKATGGG
                     DSPSRRRIFVCRPASTADELPCARRILSTLARRAYRRPANETDVSDLLPFYQRGRTEG
                     SFDLGIQKALERLLVSPQFLFRIEREPFDSAQGKPSSAAAGTAYRVSDIELASRLSFF
                     IWSSIPDQELLDVAAAGRLKEAKVLEQQVRRMLADPRSESLVTNFAAQWLYLRDIASK
                     QPDEILFADFDETLRTAMQRETELFIGSVFREDRSLLDLLRANYTFLNERLARHYGVP
                     NIKGSYFRRVTFPDGSVRGGLLGQGSLLTITSYSTRTSPVLRGKWVLENLLSAAPPPP
                     PADVPSLKTETAPGKPLTLREAMIQHRAAPACAGCHARMDPIGFAMENFDAVGRWRER
                     DGQQPIDATGVFPEGTKFEGIPGLKQELLRQPEQFVGTVAERLLMYAIGRNLQYYDAP
                     TVRAVMRDGEPANHTLASLVLGIVKSRPFQMREAGGE"
     gene            1466..>2066
                     /locus_tag="AC16CY_00545"
     CDS             1466..>2066
                     /locus_tag="AC16CY_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3352289.1"
                     /translation="MFVSRKSLPRRTFLRAMGTTIALPFLDAMVPALTARALKSTPRF
                     GFVYIANGVIQNLWTPATTGSGFELTPTLSPFASLRDKITVLSGLSHLQADTFGDGTG
                     DHPRASAVWLTGVHAYDRTQPGVEVKLATTVDQLLVREIGRTSRIPSIEMSVDFPTQG
                     SCDSGDCFYVNTVSWRNETTPNNTESHPRILFERLFGDGG"
BASE COUNT          342 a          697 c          685 g          342 t
ORIGIN      
        1 ttggcgtcgc gttcgtgcag cgcaccgagg cgcgcgacga ggcgacgctt cgcccccgga
       61 tgcgcagccg cggcacgcag ccggccatca acagcgtcac gatcagcgga ccgttcaagg
      121 ccacaggtgg cggcgattcg ccaagccggc gccgcatctt cgtctgtcgt ccggcctcga
      181 cggcggatga acttccgtgc gcgcggcgga ttctgtccac gctcgcgcgc cgtgcctatc
      241 gccgcccggc gaacgagacg gacgtcagcg acctcctgcc gttctaccag cgcggtcgca
      301 cggagggcag cttcgatctc gggattcaga aggcgctcga gcgcctgctt gtgagcccgc
      361 agttcctctt ccgcatcgag cgggagccct tcgactctgc tcagggcaag ccctcgagcg
      421 ccgcagcggg cacggcgtat cgcgtcagcg acatcgagct cgcgtcgcgg ctgtcgttct
      481 tcatctggag cagtatcccg gaccaggagc tgctcgacgt tgcagcggcg ggacgactga
      541 aggaggcgaa ggtactcgag cagcaggtgc gccgcatgct cgcggacccg cggtcggagt
      601 cgctcgtcac gaacttcgcg gcgcagtggc tctacctccg ggacatcgcg tcgaaacagc
      661 cggacgagat cctgttcgcc gatttcgacg aaacactccg caccgcgatg cagcgcgaga
      721 cggaactgtt cattggcagc gtgttcagag aggaccgcag cctgctcgat ctgctgcgcg
      781 cgaattacac cttcctgaac gagcggctcg cgcgtcacta cggtgtgccg aacatcaagg
      841 ggagctactt ccgtcgcgtg acgttcccgg acgggagcgt ccgcggcgga ttgctggggc
      901 aggggagcct gctgacgatc acgtcgtact cgacgcgcac gtcgcccgtg cttcgcggca
      961 agtgggtgct cgagaacctg ttgtcggcag cgccgccgcc tccgcccgcc gatgtgccat
     1021 cgctgaagac ggagacggcc ccgggcaagc cgctgacgct ccgcgaggcg atgatccagc
     1081 accgcgcggc accggcatgc gccggctgcc acgcgcgcat ggatccgatt ggcttcgcga
     1141 tggagaactt cgacgcggtc gggcgatggc gcgagcgcga cggccagcag ccgatcgacg
     1201 ccaccggcgt cttcccggag ggcacgaaat tcgagggaat tcccggactg aagcaggaac
     1261 tcctgcgcca gcccgagcag ttcgtcggca ccgtcgcgga gcggttgctg atgtacgcga
     1321 ttggccgcaa cctgcaatac tatgacgcac ccaccgtgcg cgccgtgatg cgcgacggcg
     1381 agccggcgaa ccatacgctg gcctcgctgg tgctcggaat cgtgaagagc cgtccgttcc
     1441 agatgcgcga ggctggaggc gagtgatgtt cgtgagcagg aagtcgctgc cgcgtcggac
     1501 gttcctgcgt gccatgggca cgacgatcgc gttgccgttt ctcgacgcca tggtcccggc
     1561 gctcacggca cgggccctca aatccactcc gcgcttcggt ttcgtctaca tcgccaacgg
     1621 cgtcatccag aacctgtgga cgccggcaac gaccggatcc gggttcgagc tgaccccgac
     1681 gctctcgcct ttcgcgagcc tgcgcgacaa gatcacggtc ctcagtggcc tctcgcacct
     1741 gcaggccgac accttcggcg acggcacggg cgatcatccg cgcgcgtcgg cggtgtggct
     1801 gaccggcgta cacgcttacg accgtacaca gccaggcgtc gaggtgaagc tggcgacgac
     1861 ggtcgatcag ttgctggtgc gcgagattgg caggacgtcg cgcattccgt ccatcgagat
     1921 gtccgtcgat ttcccgactc agggctcctg cgactcaggc gactgcttct acgtgaacac
     1981 tgtctcgtgg cgcaacgaga cgacgccaaa caacaccgag tcgcatccgc ggattctgtt
     2041 cgagcgcctg ttcggcgacg gcggca
//
LOCUS       DBNGSH010000026         3346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3227774, whole genome shotgun sequence.
ACCESSION   DBNGSH010000026 DBNGSH010000000
VERSION     DBNGSH010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3227774"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /gene="lptC"
                     /locus_tag="AC16CY_00550"
     CDS             complement(<1..664)
                     /gene="lptC"
                     /locus_tag="AC16CY_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR3352290.1"
                     /translation="MTSWQKRVRFGIAVLGIAFAAFVYWSTGARRAAVPSQAVERIDP
                     KAVIETTAATRRETRGKDRTFDITWSRFLTYGDGSTRVFDVVITVYRPENRTVTITAK
                     EAFNGPNETRRQLMGEVTLSTSSGFTMLTDEATVDENTGVTRVPGGLTFAKGGMSGSG
                     LGATYEEATDVLRIESQVKVIVKDRSGRQTMEGRSGSATMERLQDVVLLDSAVHLLRE
                     GEV"
     gene            complement(717..2435)
                     /gene="recJ"
                     /locus_tag="AC16CY_00555"
     CDS             complement(717..2435)
                     /gene="recJ"
                     /locus_tag="AC16CY_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR3352291.1"
                     /translation="MIQRLWQPLSCDDAQAGILARELNIAPVTARLLCIRGIADAGEA
                     RRFLTPSLDDLLDPFTLTDMPVAVDRILRAIAGRERIAVHGDYDVDGVTSTVILRRAL
                     ELLGADVTHFIPERVRDGYGLQPASLDRLAAEGVRLVISVDCGIRGVEAAARARALGL
                     DLIITDHHEPDVELPDALAVINPKRHDCAYPEKNLAGVGVALKVVQALCARTERSSWL
                     PAFVKMAAIGTLADVVPLTGENRVIAKVGLGLLSKGPHKIGLRALLEVCGLTGKEIDS
                     YHIGFVLAPRVNAAGRMSTPDIAARLLLASDEAMADEARALAVQLDAENVRRQQEEAD
                     LVAQARKMVETDIEIGSRTVIVVGGEGWHRGVIGIVASKLVDAFHRPAIVISLDGGTA
                     HGSCRSIPSFNMLAALESCAEIMTRFGGHKQAAGLTIDSSRIRELRARVNDYADARLQ
                     PDDLRPRIWIDGGLDFRSINEQVASELTTLAPFGAGNPRPIFHASRVEVVDGPRRVKD
                     RHLKMAFRQDGRVMRGIAWRAIERETFVTEHRSSIDLAFSLEQDTWNGDRYLQLSIAD
                     FKAPEN"
     gene            complement(2432..>3346)
                     /locus_tag="AC16CY_00560"
     CDS             complement(2432..>3346)
                     /locus_tag="AC16CY_00560"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR3352292.1"
                     /translation="KEIEALSMLPVTAERQRSIDGLVTRIDSIRRDIYRTLTPWQRVQ
                     VARHPSRPTVLDYVNRLFTDFTEISGDRRFADDHAIVAGMARYKQEPVLVVGHHKGGG
                     GDTKQKIYRNFGYAKPEGYRKALRAMKLAEKFQRAVIAFIDTPAAYPGIESEERGISE
                     AIALNLREMASLDIPIIVVVHGEGGSGGALGLAVGDRILMNQFAIYSVIPPEGCAAIL
                     WRDSGRKVEAAEALKLTAPDLLSLGLIDEIVPEPVGGSHTNPDQAAALLDPSLQRALA
                     EVTSMNPAARLDERYRKWRRMGDVGIAE"
BASE COUNT          531 a         1176 c         1063 g          576 t
ORIGIN      
        1 tcacttcgcc ttcgcgaagg agatgcaccg cggaatcgag gagcacgacg tcctgaaggc
       61 gctccattgt cgcgctgccg gagcgcccct ccatggtctg ccgccccgat ctgtctttca
      121 cgatgacttt gacctggctt tcgatccgca acacgtcggt ggcctcttcg taggtcgctc
      181 cgagacccga gccgctcatc ccgcccttcg cgaacgtcag gccgccgggc acccgcgtca
      241 ctcccgtgtt ctcgtccacg gtcgcctcgt ccgtcagcat cgtgaaaccg ctgctcgtcg
      301 agagcgtcac ctcgcccatc agttgccgtc gcgtctcgtt cggaccgttg aacgcttcct
      361 tcgccgtaat cgtgaccgtt cgattctcgg gcctgtacac cgtgatcacg acgtcgaaca
      421 cgcgggtcga gccgtcgcca taggtcagga accggctcca cgtgatgtcg aaggtccggt
      481 ccttcccgcg agtttcgcga cgcgtcgcgg ccgtcgtctc gatgacggcc ttcggatcga
      541 tccgctccac cgcctgtgac ggaacggccg cccggcgtgc cccggtcgac cagtacacga
      601 atgccgcgaa cgcaatccca aggacggcga tcccgaaccg aacgcgcttc tgccaggacg
      661 tcatgccgcg cgtccagaat agcacctcga acgcggcgtg ccgcccggcg gcgcggtcag
      721 ttctcgggcg ctttgaagtc ggcgatggag agctggaggt agcggtcgcc gttccaggtg
      781 tcctgctcga gcgagaacgc caggtctatg gacgaccgat gttcggtcac gaacgtctcg
      841 cgctcgatcg cacgccaggc gatgcccctc atgacgcggc cgtcctgccg gaacgccatc
      901 ttcaaatggc gatccttgac gcggcgggga ccatccacga cctcgacgcg gctggcgtga
      961 aagatggggc gcgggttgcc ggcgccgaac ggcgccagcg tcgtgagctc ggaggcgacc
     1021 tgctcgttga tcgatcgaaa gtcgagaccg ccgtcgatcc agatccgcgg gcgcaggtcg
     1081 tcgggctgaa gacgagcgtc cgcgtagtca ttcactcgtg cacgcagctc ccggattcgc
     1141 gaggagtcga tcgtcaggcc ggccgcctgc ttgtggcctc cgaacctggt catgatctcg
     1201 gcgcacgact cgagcgccgc cagcatgttg aacgacggga tgctccggca ggacccgtgt
     1261 gccgtgccgc catcgaggga gatgacgatt gccgggcgat gaaacgcatc caccagcttc
     1321 gaggccacga tgccgatcac gccccggtgc cagccctcgc ctccgacgac gatgaccgtc
     1381 ctggacccga tctcgatgtc ggtctccacc atcttccgcg cctgggcgac gaggtcggcc
     1441 tcttcctgct gccggcggac gttttcggcg tcgagctgca cggccagcgc acgcgcttcg
     1501 tcggccatcg cctcgtcgga cgcaagcagc agccgtgccg caatgtccgg cgtgctcatc
     1561 cggcccgccg cgttgaccct cggcgccagc acgaagccga tgtggtagct gtcgatctcc
     1621 ttgcctgtga ggccgcacac ctcgagcagg gcccgtaaac cgatcttgtg cggccccttc
     1681 gaaagcaaac cgagccccac tttggcgatc acccggttct cgcccgtcag gggcacgacg
     1741 tcggcaagcg tgccgatggc ggccatcttg acgaacgcag gcagccagct gcttcgttcc
     1801 gtccttgcgc acagcgcctg cacgaccttc agcgccacgc ccacgcccgc cagattcttt
     1861 tccggatacg cgcagtcgtg gcgcttcggg ttgatcacgg ccagcgcatc cggcaactcg
     1921 acgtcgggtt cgtggtgatc cgtgatgatc aggtcgaggc cgagcgccct ggcgcgtgca
     1981 gccgcctcca cacccctgat gccgcagtcc accgagatga ccaggcgcac gccctcggcg
     2041 gcgagcctgt cgagcgacgc cggctgcagg ccgtacccgt cgcgcacgcg ttcggggatg
     2101 aaatgcgtca cgtccgcgcc cagcaactcg agcgcacgtc gaagaatgac cgtggaggtc
     2161 acgccgtcaa cgtcgtagtc cccgtgaacg gcgatccgct cgcgaccggc gatggctcgc
     2221 agaatgcgat cgaccgccac gggcatgtcg gtgagcgtga acgggtcgag caggtcgtcg
     2281 agcgacggtg tgaggaaccg cctggcctca cccgcgtccg cgattccacg gatgcagagg
     2341 agccgggccg tcaccggggc aatgttcagt tcgcgcgcca atatgccagc ctgggcgtcg
     2401 tcacacgaga gcggctgcca gaggcgctgg atcattcggc gatccctacg tcgcccatcc
     2461 gccgccactt ccggtaccgc tcgtccagcc gggccgccgg attcatcgac gtgacctccg
     2521 caagtgccct ctggagcgac ggatcgagca gcgcggccgc ctggtccgga ttggtgtgcg
     2581 atccgccgac gggctcaggc acgatttcgt caatcagccc gagcgacagc agatcgggtg
     2641 cggtcagctt gagcgcctcc gccgcctcga ccttgcggcc cgagtcgcgc cagaggatcg
     2701 ccgcacaccc ttccggggga atcacgctgt agatcgcaaa ctggttcatc aggatccggt
     2761 cgccgacggc gagccccagc gctccaccgc tgccgccctc gccatgcacg acgacgataa
     2821 tggggatgtc gagcgacgcc atctcccgaa ggttcaaagc gatggcctcc gagatcccgc
     2881 gctcctcgga ctcgatgccg ggatacgcgg cgggagtgtc gatgaacgca atcaccgctc
     2941 gctggaactt ctcggccagc ttcatggccc gcagcgcctt gcgatagccc tcgggcttgg
     3001 cgtagccgaa gttgcggtag atcttctgct tcgtgtcccc tcccccgccc ttgtggtgcc
     3061 ccacgaccag gacgggctcc tgcttgtagc gggccatgcc ggccacgatg gcgtgatcgt
     3121 cggcgaaccg tcggtcgccg ctgatctcgg tgaagtcggt gaaaagacgg ttgacgtagt
     3181 ccagcaccgt cggccggctc ggatgccgcg cgacctgcac gcgctgccag ggtgtcagcg
     3241 tccggtagat gtcccgccgg atggagtcga tgcgcgtgac gaggccgtcg atcgagcgct
     3301 ggcgctctgc cgtcaccggg agcatcgaca gcgcctcgat ctcctt
//
LOCUS       DBNGSH010000027         7019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3907117, whole genome shotgun sequence.
ACCESSION   DBNGSH010000027 DBNGSH010000000
VERSION     DBNGSH010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3907117"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="AC16CY_00565"
     CDS             complement(<1..405)
                     /locus_tag="AC16CY_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352293.1"
                     /translation="MIPGVRGRLLAASFIRDVLPTMAGVGELPRDVTRDLARWCARIE
                     STLGTASSVRAIADVAIVPLLDLLGLRVARRADTGDACWIRLDAGSRSASIAIVTPWG
                     ARLEGVWRSAVVEAIGADARWCFCSNGLVLRLV"
     gene            complement(402..2795)
                     /locus_tag="AC16CY_00570"
     CDS             complement(402..2795)
                     /locus_tag="AC16CY_00570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3352294.1"
                     /translation="MAAPVPVPGDLVRIRDERWTVTRHAPGLDAGVLEVRGRDRTNFG
                     ARASFLLPFDIVEPLPSSHAPRAARPRSWRRLTRSMLARATPSWGSLRTPLHARVALL
                     AFQLEPALAVTHGLASRVLIADEVGLGKTVQAGLVIGEILDRSSRARVLIVAPASLRE
                     QWRTELEDRFGLGAWVADSASLARAGATWTDVNPWRLHPITITSIDYVKRPEALRALD
                     AIVWDAVVFDEAHALAGRSDRAAAAAALARRARTVVMLTATPHTGDDDAFDRLCRIGD
                     LDGGFPLLLFRRTRQDVGLAVSRKSSTLRVRPSLAESAMHGALMTYARRVWAHECSPS
                     GSARLAMSVLVRRASSSAASLARSVERRLALLAGTEHGPQQLGLPLGDMSADEEPGAE
                     LAAPGLADREDERRRLEHVLRAARRAEPSESKLRLLSRFLRRTRDPAIVFTEYRDTLE
                     RLAVTLREHDPVTLHGGLTAAERRDSLQRFTAGTARLLIATDAASEGLNLQARCRLVI
                     NLELPWTPLRLEQRIGRVDRIGQPRRVHAVHLVAANTPEESTVARLLLRTRRAAAVLD
                     GLRPRATEQDIAGTVIGRERFDDAAPELRVPQVLAPGVSSLASSEAARLEKLRSVAGH
                     QSSAPADDRPPVVVRRGMGRRGILLGFRLVFVDTDRRPVWETFLGVEACGPAPWLDSR
                     YARWIELSAPRLEQTIAIEGRRAVAALEASLRTPLALASRRERAIASSIAADRARLAA
                     DLLQPGLFDRRAERLRAAQAAVLDEALAHCRAHLETISGTGHPVIERLHLAFAVIRR"
     gene            2931..3188
                     /locus_tag="AC16CY_00575"
     CDS             2931..3188
                     /locus_tag="AC16CY_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR3352295.1"
                     /translation="MRIPIEDTLDLHAFAPRDVQSVVEEYVTAAHDAGFREVRLIHGR
                     GKGVRRGLVQQALERHPLVVEFWDATETHLGATVARLAGSR"
     gene            complement(3217..3564)
                     /gene="queF"
                     /locus_tag="AC16CY_00580"
     CDS             complement(3217..3564)
                     /gene="queF"
                     /locus_tag="AC16CY_00580"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR3352296.1"
                     /translation="MSSTATIETFPSPRPDREFEISISCPEFTSVCPKTGLPDFGEIR
                     ITYVPGDRCIELKSLKYYMIGFRDRGIFYEAATNQILDDLVQACQPRRMTVVGDFTVR
                     GGIKTVVKASYPA"
     gene            3761..4042
                     /locus_tag="AC16CY_00585"
     CDS             3761..4042
                     /locus_tag="AC16CY_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3352297.1"
                     /translation="MGKSELFSHFAERFEVKRTQAREFFDELTTLSEKELKRSGEFVL
                     PGMVKLVVQKRKARMGRNPATGEAIKIPAKTVVKARIAKQLKDAVLPKK"
     gene            complement(4118..5506)
                     /locus_tag="AC16CY_00590"
     CDS             complement(4118..5506)
                     /locus_tag="AC16CY_00590"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3352298.1"
                     /translation="MADLRKTLLIVDSEEETRDAITATLRRDYRVLRAATGETALHMM
                     QKESVDLLLLDVHLPGISGFEVLKITKENYPRIEVVVLSRTKELDTAIEAMRHGAYHF
                     ISKDFDAEAIRACFANAAERQDLSRDVTRLREEVAEQNEREFVVGPSRTTRSVVELVN
                     KVAKLSATVLILGESGTGKELLARLIHRESGRPGAPFVAVNLAAIPKELVESALFGHE
                     KGAFTGAIRQQIGKFELAAGGTLFLDEIGDLRYEVQAKLLRAIQEEEIERVGGTHPIK
                     TDLRLIAATNVDLDKAVKEGRFREDLYYRLNVIPIRMPALRDRMEDLPDLARFFLRRY
                     NTKFRKNIQGIAESTLDVLNAYWWPGNIRELENLIERLVAVSDKDWITDDDLPYELHM
                     ARLDNQGSPGENLLERALATFERNFIVRALEKSAWNVTATARTLGLPLSTLKFKMDKL
                     EIRELARKIRGN"
     gene            5595..6845
                     /locus_tag="AC16CY_00595"
     CDS             5595..6845
                     /locus_tag="AC16CY_00595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3352299.1"
                     /translation="MSTPVRMPQMGESIAEGTIVRWIKKVGDTVGIDEPLFEISTDKV
                     DAEIPAPAAGVLIEIKVKEGETVAVDSVVATIGQASEVPTPNAQLPTPESPIPSPGPR
                     AQSREPRAESPESRVESPEPAGLRTSPFVRRVAREHNVDVTRIKGSGLGGRVTKQDIL
                     EYLEKPQASGLRPQGSGLRPQEVPGPQSGVEIKPLSVMRKKIAEHMIMSRRTSAHVHS
                     VFHVDFSTVERIRRERKADYERLNTKLTYMAFIAKAVVTSLRRFPIVNASLDGDNILY
                     KKDINLGIAVALEGGLIVPVIRSADEKNLLGLSRAIADVAERARTKQLKPEEVHDGTF
                     TITNPGQFGSQFGMPIINQPQVAILGVGVIEKRPVVVDEAIAIRTMGYLTLGHDHRLI
                     DGATGDQFMAEIKQQIERFDPSQV"
BASE COUNT         1212 a         2317 c         2401 g         1089 t
ORIGIN      
        1 caccagccgc agaacgaggc cgttggagca gaaacaccat cgagcgtccg cgccgatcgc
       61 ttccacgact gccgatcgcc acacgccttc cagccgtgcg ccccacggcg tgacgatcgc
      121 gatggacgcc gatcgtgacc ccgcatccag tcggatccag caggcgtcgc cggtatcggc
      181 gcgcctcgcg accctcaggc cgagcagatc gagcagcggg acgatcgcca cgtccgcgat
      241 cgcgcggacg ctggaggcag tcccgagcgt ggattcgatc cgggcgcacc accgcgcaag
      301 atcccgcgtg acgtcccgtg gcagctcacc cacgcccgcc atcgtcggga gcacgtcgcg
      361 gatgaatgaa gcggcgagca gccgcccgcg gacgccggga atcatctgcg aatcaccgcg
      421 aaggcgagat gcaggcgctc gatgaccgga tgcccggtgc ccgaaatcgt ctccaggtgc
      481 gcgcggcagt gagccagcgc ctcgtcgagc actgcggcct gcgccgcccg caggcgttcg
      541 gcccggcgat cgaacagacc cggctgcagg agatcggccg ccagcctcgc acggtcggcc
      601 gctatcgagc tggcgatggc ccgttcacgg cgggaggcga gcgccagcgg cgtccgcaac
      661 gacgcttcga gcgccgcaac tgctcgtctc ccctcgatcg cgatcgtctg ctcgagacgt
      721 ggcgccgaca actcgatcca ccgtgcgtaa cgggagtcga gccacggcgc gggaccgcat
      781 gcctcgaccc ccaggaacgt ctcccacacc ggccgccgat ccgtgtccac gaacacgagc
      841 cggaagccca acaggattcc gcgacgaccc atgccgcggc ggacgaccac gggaggccga
      901 tcatcggcgg gcgcggaaga ctggtgcccg gccacggagc gcagcttctc gaggcgggcg
      961 gcttctgacg acgccaggga ggaaacgccc ggtgcgagaa cctgcggcac tcggagttcc
     1021 ggagctgcat catcgaacct ttcgcgcccg atgacggtgc cggcgatgtc ctgttcggtc
     1081 gctcgcgggc ggagaccgtc gaggacggcg gccgcgcgcc gggttcggag cagcagcctc
     1141 gcgaccgtgg actcttcggg tgtgttcgcc gcgacgagat gcaccgcatg aacccggcga
     1201 ggctgaccga tgcgatcgac gcggccgatg cgctgctcga gccgaagagg cgtccacggg
     1261 agctcgaggt tgatgacgag gcggcatcgc gcctggaggt tgaggccttc gctggcggca
     1321 tcggttgcga tgagcagccg tgcggtgccg gcagtaaagc gttgcaggct gtcgcgccgc
     1381 tcggccgcgg tgagccctcc gtgcagcgtc accggatcgt gttcgcgcag cgtgacggca
     1441 agccgctcga gcgtgtcgcg atactcggtg aaaacgatgg ccgggtcgcg cgtccggcgc
     1501 aggaagcgcg acagcagccg aagcttggat tccgagggct cggcccgccg cgccgctctc
     1561 aggacgtgtt cgagacgccg ccgctcatcc tcccgatccg caaggccggg cgcggccagt
     1621 tccgctcccg gctcctcgtc ggcggacatg tcgccaagcg ggagccccag ctgctgcgga
     1681 ccgtgttcgg ttcccgccag cagcgccagc cttcgctcga ccgacctggc gagcgacgcc
     1741 gcgctgctcg atgcacggcg gaccagaacc gacatcgcga ggcgggcgga gccggacggc
     1801 gagcactcgt gcgcccacac cctgcgggcg tacgtcatca gcgcgccgtg catcgccgac
     1861 tcggcgagcg acgggcgtac tcgcagggtt gaagactttc gcgagacggc aaggccgacg
     1921 tcctgccgcg tccggcggaa cagcagcagc gggaaccctc cgtcgaggtc gccgattcgg
     1981 cagaggcgat cgaaggcgtc gtcgtcgccg gtgtgcggcg tcgccgtcag catgacgacc
     2041 gtccgcgcgc ggcgggcgag tgccgccgcg gcggcggccc gatccgatcg ccccgcgagc
     2101 gcgtgcgctt cgtcgaagac gacggcgtcc cagacgattg cgtcgagggc ccgcagcgct
     2161 tcggggcgct tcacgtagtc gatcgaggtg atcgtgatcg gatgcaggcg ccatggattc
     2221 acgtccgtcc atgtggcgcc cgcccgggca agcgaggccg aatcggcgac ccatgcgccg
     2281 aggccgaagc gatcctccag ctcggtgcgc cactgttctc tcaaggacgc gggcgcgacg
     2341 atcagcacgc gggcgcggga ggagcggtcg agaatttccc cgatgacgag gcccgcctgc
     2401 acggtcttgc cgaggccgac ctcgtcggca atcaggactc gcgacgcgag cccgtgcgtg
     2461 acggcgagcg ccggctcgag ctgaaaggcg aggagcgcaa cgcgcgcgtg gagcggcgtg
     2521 cgcagcgagc cccaggacgg cgtggcgcgc gcgagcatcg agcgcgtgag ccgccgccac
     2581 gaccttggtc gagccgcgcg cggcgcgtgc gaggagggaa gcggctcgac gatgtcgaac
     2641 gggagcagga acgaggcgcg cgcaccgaag ttggtccgat cccggccgcg cacctccagc
     2701 accccggcgt cgaggcccgg cgcgtggcgg gtgacggtcc agcgctcgtc acggatgcgg
     2761 acgaggtcgc cgggaacagg aacgggcgcc gccatcgacc ggtgtggtgc aggggccggt
     2821 cccgcggccg aatggcggga aaacgtgaag aggccgtttg aatgttgaga aaactgcgcg
     2881 cccgattcgc gccagcggta ggagaatgtg ctaccagctg ccggctagag atgcgaatcc
     2941 cgatcgagga cacgctcgac cttcacgcct tcgcgccgcg cgacgtccag tccgtcgtcg
     3001 aggagtacgt caccgcggcg cacgacgcgg ggttcagaga agtgcgcctg atacacggcc
     3061 gcggcaaggg cgtccggcgc gggctcgtgc agcaagcgct ggagcgtcac ccgctcgtcg
     3121 tcgaattctg ggacgcaact gagacccacc ttggcgcgac ggtagcgaga ctggccggga
     3181 gccgataact ggtcgtcggt agcctgcccg ccatggtcaa gctggatacg acgccttcac
     3241 gacggtcttg atccccccgc ggacggtgaa gtcgccgacg acggtcatcc ggcgcggttg
     3301 gcaggcctgg accaggtcgt cgaggatctg gttggtcgct gcctcgtaga agatgccgcg
     3361 gtcgcggaac ccgatcatgt agtacttcaa cgacttcagc tcgatgcagc gatcgccagg
     3421 cacgtaagtg attcggatct cgccaaagtc ggggaggccg gttttcgggc agacggacgt
     3481 gaactcagga cagctgatcg agatctcgaa ctcccgatcc ggtcgaggac tcgggaaggt
     3541 ctcgatcgtc gctgtgcttg acatggggaa aaaattctag cacttcaggc cgaaatcgac
     3601 cgatcgtgcg gcgtgtttcg ttgacagcgt atcgggtgcg cgctagcatt acgcgatttc
     3661 actagagttt ccgcggcagt gaagagcgcg cgcaactgcg ttgcggccta aaggtcggcg
     3721 cgcacaagac tggggggtcg tgatggcaga ggcccggaga atgggcaaat cggaattgtt
     3781 ctcgcacttc gccgaacggt tcgaggtgaa gcgtacccag gcgagagagt tcttcgacga
     3841 gttgaccacg ctttcagaaa aggaactcaa gcgctcggga gagttcgtac tgccgggcat
     3901 ggtgaagctg gtcgtccaga agcggaaggc ccgcatgggc cgcaaccccg cgacgggcga
     3961 ggccatcaag attccggcga agacggtcgt caaggcacgt atcgccaagc aactcaagga
     4021 cgcggtgctt cccaagaagt agctcgacgc actacggcgg aacgttccgc ccagttcccc
     4081 cctgtgaccc ggcgcgccag aacgcgccgg gttccgttca atttcccctg attttgcgcg
     4141 cgagctcgcg tatctcgagc ttgtccatct tgaacttcag cgtgctcagc ggaaggccca
     4201 gcgttcgggc cgtcgcggtc acgttccacg cggacttctc gagggcgcgc acgatgaagt
     4261 tgcgctcgaa ggtcgcgagc gcgcgttcga gcaggttttc gcccggcgat ccctggttgt
     4321 cgagcctggc catgtgcagc tcgtagggca ggtcgtcgtc ggtgatccag tccttgtcag
     4381 acacggccac gagccgctcg atcaggtttt cgagctcgcg gatgttgccc ggccaccagt
     4441 acgcgttgag gacgtcgagc gtggattcag cgatgccctg gatgttcttc cggaacttcg
     4501 tgttgtagcg tcggaggaag aagcgcgcca gatcgggcag gtcctccatc cggtcgcgca
     4561 gcgccggcat gcggatcggg atcacgttga ggcggtagta gaggtcctcg cggaaccggc
     4621 cttccttcac cgccttgtcc agatcgacgt tggtggcggc aatgagccgg agatcggtct
     4681 tgatgggatg tgtgccgccc acgcgctcga tctcttcctc ctggatcgcg cggagcagct
     4741 tcgcctgcac ctcgtagcgg agatcgccga tctcgtcgag gaagagcgtg ccgccggcgg
     4801 cgagctcgaa cttgccgatc tgctggcgga tggcgcccgt gaaggcgccc ttttcgtggc
     4861 cgaacagcgc gctctcgacg agctctttcg gtatggccgc caggttgacg gcgacaaacg
     4921 gcgcgccggg ccggcccgat tcgcgatgga tgagccgcgc gagcagctcc ttgccggtgc
     4981 cgctctcgcc gaggatgagc acggttgccg acagcttggc caccttgttg accagctcga
     5041 ccaccgaccg cgtcgtgcgg ctcgggccga cgacgaactc gcgctcgttc tgctcggcga
     5101 cttcttcgcg cagccgcgtg acgtcccggc tgagatcctg ccgctcggcg gcgttggcga
     5161 agcacgcgcg gattgcctcg gcgtcgaagt ccttcgagat gaagtgatac gccccgtgac
     5221 gcattgcctc gatcgccgtg tccagctcct tggttctcga cagcaccacg acctcgatcc
     5281 gcggatagtt ctccttggtg atcttgagga cctcgaatcc gctgatgccg gggaggtgca
     5341 cgtccagcag caacaggtcg acgctctcct tctgcatcat gtggagagcg gtttcgcccg
     5401 tggcggcccg cagcacgcga tagtcgcggc gcagcgtcgc cgtgatcgcg tcgcgcgtct
     5461 cttcctcgct gtcgacgatg agcagggttt tccggaggtc cgccattggg gggtcgccgt
     5521 gtattctagc gaaccccgta tactggggtc agaccccatg acacgcatcg actcatgggg
     5581 tctgacccca gacgatgtca acaccggtcc gcatgcccca gatgggcgag tcgatcgccg
     5641 agggcacgat cgtgcgatgg atcaagaagg tgggggacac cgtcggcatc gacgagccgc
     5701 tcttcgagat ctccaccgac aaggtcgatg cggagatacc ggcgccagcg gccggcgttc
     5761 tcatcgagat caaggtgaag gagggcgaga ccgtcgcggt ggacagcgtg gtggccacca
     5821 tcgggcaagc gtccgaagtt ccaacgccca acgcccaact cccaacgccc gaatccccaa
     5881 ttccgagccc agggccgaga gcccagagcc gagaaccgag agccgaaagc ccggagtcca
     5941 gggtcgagag cccagagccg gccggcctgc ggacctctcc cttcgtccgg cgggtcgcgc
     6001 gcgagcacaa cgtggacgtg acccgcatca agggctcggg cctcggcggc cgcgtcacca
     6061 agcaggacat cctggaatac ctggagaagc cccaggcctc aggcctcagg ccccagggct
     6121 cagggctcag gcctcaggag gttcccggtc cccaatccgg cgtcgagatc aagccgctgt
     6181 cggtgatgcg caagaagatc gccgagcaca tgatcatgag ccgtcgcacg tcggctcacg
     6241 tgcactcggt gtttcacgtg gacttctcga ccgtcgagcg catcaggcgc gagaggaaag
     6301 cagattacga acgcctcaac acgaagttga cctacatggc gttcatcgcg aaggcggtcg
     6361 tcacgtcgct ccgtcgcttt ccgatcgtca acgcgtcgct cgacggcgac aacatcctct
     6421 acaagaagga tatcaacctc ggcatcgccg tcgcgctcga gggcggtctg atcgtgccgg
     6481 ttatcaggtc cgccgacgaa aagaacctgc tcggcctcag ccgcgcgatc gccgacgtcg
     6541 ccgagcgcgc gaggaccaag cagctcaagc cggaagaggt gcacgacggc accttcacca
     6601 tcaccaatcc cggtcagttc ggctcccagt tcggcatgcc gatcatcaac cagccgcagg
     6661 tggcgattct cggcgtcggc gtcatcgaga agcggccggt cgtcgtggac gaagcgattg
     6721 ccatccggac gatggggtat ctgacgctgg gccacgacca caggctgatc gacggcgcga
     6781 ccggcgatca gttcatggcg gagatcaagc agcagatcga gcgcttcgac ccgagccagg
     6841 tttgacaaga ccgttggagg tgcggcgcct gggcgtcgtg cggtacggcg aggccctcga
     6901 gatgcagcgc gcgctcgtgg aagagcggcg cgccggccgc gttcccgatc tccttctcct
     6961 tcttcagcac ccgcccgtca tcacgctcgg cgttcgcgcc gacgccgcgc gcgccaaca
//
LOCUS       DBNGSH010000028         1993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1190270, whole genome shotgun sequence.
ACCESSION   DBNGSH010000028 DBNGSH010000000
VERSION     DBNGSH010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1190270"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..796
                     /locus_tag="AC16CY_00600"
     CDS             194..796
                     /locus_tag="AC16CY_00600"
                     /inference="COORDINATES: protein motif:HMM:NF025679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HIR3352300.1"
                     /translation="MQRTLALALVLAFAAFGCDSTSGPSGPAGTLNLRLTDSPFTDAD
                     AVLVTFTGVRVHRAESDWETLPFADAAAVSRTCDLKKLQNGAEDILGTGPLVAGQYTM
                     IRLVVESAKLYFDNPTAAGPACATTITPPAGASADVTVSSGEVKLNRPFTLTADTATT
                     ILLDLDGNGSIHQQGNGSYRMTPVIGIVSVTAQTPPPAQP"
     gene            complement(889..1938)
                     /locus_tag="AC16CY_00605"
     CDS             complement(889..1938)
                     /locus_tag="AC16CY_00605"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR3352301.1"
                     /translation="MKGIASLLGEHASLLDHQCRTIPKENLLLPGPDYVDRAYGLSDR
                     SPRVLGAIQSMLDTGRLAGTGYVSILPVDQGIEHSAGASFAPNPAYFDPENIVKLAIE
                     GGCNAVASTLGVLGSCSRKYAHKIPFILKFNHNEFISYPNSYDQIRFASVKQAFDMGA
                     MAVGATVYFGSEESKRQIQEVTELFQRAHELGMFTVLWCYLRNKAFKTKEVDYHQAAD
                     LTGQANHLGVTIEADIIKQKMPETNGGYTALEFGKTHKAVYSQLTSDNPIDLTRYQLA
                     NCYMGRAGLINSGGASSGEGDVKEAVRTAVINKRAGGMGLISGRKAFQRPMKEGAALL
                     NAIQDVYLTKEVTIA"
BASE COUNT          342 a          637 c          633 g          381 t
ORIGIN      
        1 ctcgaggaat tcttccgggc tggctgaccc tcttcgccca ttaccaaatt acatactcgg
       61 ggttcgtatc cgtggctcaa cgggggactc gaacgagcgc gtttcgcccg gaacttccac
      121 gaagccatta acttagagtt cgccgggcca ctggaacccg tattgctact ctccgggctt
      181 tgggagatta gacatgcaac gcacattggc actcgccctg gtgctcgctt ttgcagcctt
      241 cggctgcgac tcgacgtccg gtccctcagg accggcggga acactgaacc tgcgcctgac
      301 cgacagtccg tttaccgacg cagacgccgt gctcgtgacg ttcaccggcg tgagggtcca
      361 tcgcgcggag agtgattggg agaccttgcc gttcgcggat gccgccgcag tttctcgtac
      421 ctgcgacctg aagaagcttc agaacggggc ggaggacatc ctcggaaccg gaccgctggt
      481 tgccgggcag tacacgatga ttcggctggt cgtcgagagt gcgaagctct acttcgacaa
      541 cccgacagcg gctggaccgg cgtgcgcgac cacgattacg ccgccggcgg gcgcttcggc
      601 ggacgtcacg gtttcttccg gcgaggtgaa gctcaatcgc ccgttcacgc tcacggccga
      661 caccgcgacg acgatcctcc tcgacctgga tggcaacggt tccatccatc agcaggggaa
      721 cgggagctac agaatgacgc cggtgatcgg gatcgtcagc gtgacggccc agacgcctcc
      781 gccggcgcag ccgtaactcg aagaccgatc gcgcgggctg aagcccgcgc tctaccgcgc
      841 gccgataggg accgcagagt agagcgctgg cttcagccag cgcggcgctc acgcgatcgt
      901 cacttccttc gtcagataca cgtcctggat cgcgttgagc agcgccgcgc cctctttcat
      961 cggacgctgg aacgccttgc gccctgagat gaggcccatg ccgccggctc gcttgttgat
     1021 gacggcggtc cgcacggctt cctttacgtc tccttcgccg gacgaggcgc cgccggagtt
     1081 gatgaggccg gcgcgaccca tgtagcagtt ggcgagctga taacgggtga gatcgatcgg
     1141 gttgtcgctc gtcagctgcg agtagaccgc cttgtgcgtc ttgccgaact cgagcgcggt
     1201 gtagccgccg ttggtctccg gcatcttctg cttgatgatg tcggcctcga tcgtcacccc
     1261 caggtggttg gcctggcccg tgagatcggc tgcctggtgg tagtcgacct ccttggtctt
     1321 aaaagccttg ttgcgcaggt agcaccagag cacggtgaac atcccaagct cgtgcgcccg
     1381 ctggaagagc tccgtcactt cctgaatctg gcgcttcgac tcctccgacc cgaagtacac
     1441 cgtcgcgccc acggccatcg cgcccatgtc gaaggcctgc ttgacgctgg caaagcgaat
     1501 ctggtcgtac gagttcggat aggagatgaa ctcgttgtga ttgaacttca ggatgaacgg
     1561 aatcttgtgc gcgtacttgc ggctgcacga gccgagcacg ccgagcgtcg aggcaacggc
     1621 gttgcacccg ccctcgatcg ccagtttcac gatgttctcc ggatcgaagt acgccgggtt
     1681 gggcgcgaag gaggcgcccg ccgagtgctc gatgccctga tcgacgggga ggatcgagac
     1741 gtagccggtt ccggccagcc gtcccgtgtc gagcatcgac tggatggcgc caagcacgcg
     1801 cggggatctg tcggacaacc cgtaggcgcg atcgacgtag tcgggacccg gcaggagcag
     1861 gttctccttc gggatcgtcc ggcactggtg gtcgagcagg ctggcgtgct caccgagaag
     1921 cgacgcaatc cccttgattc ccggcacggt ccgttcgacg gtcgcggttg aagccacgtt
     1981 cacacctccg cgc
//
LOCUS       DBNGSH010000029         3929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3567889, whole genome shotgun sequence.
ACCESSION   DBNGSH010000029 DBNGSH010000000
VERSION     DBNGSH010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3567889"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2584)
                     /locus_tag="AC16CY_00610"
     CDS             complement(<1..2584)
                     /locus_tag="AC16CY_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR3352302.1"
                     /translation="MSRVGSILVFLVSVLITITAAQEAPLTTVNSGPQGRLDSLAQAN
                     EIRIVFSEPMVALGRIPAKVDAPFVRISPAISGTFRWSGTTILIFTPDPKQPLPYATS
                     YEVTIDGATAVSGRTLARAETFRFTTPTVRLLQTNWYRRDGTIGSPMVALLRFNQPVR
                     AQDVAAHLTASLARHDWNPPAFTPEEETRLRALDPSGLKSFADKVAATRLVAAGGGAV
                     GLRLTSDWDQKRFPASPDLVAFETTSLVRPESWVRLALDAALPSPAGPATPPGEQSFT
                     IRAESAFFIEDFRCTSECDGDAWNPMRVRAPVRVADFAAALRATDITSGEQPVQKPST
                     PRPRPVYAPEASNFLTLEDAGFNAQPPNRKYVVVAAASLRAADGQTLGYPWLGIVDNW
                     HMRAFTSFGDGHGVWEKDGGAQLPFYARNLLDVRQWASALEPSQLMPRLLELQKDRFN
                     SPPAGDGVNRRLGVTPDRVQSHGLDLSGALRPGGTGLVWTAVREGELLPRTRRVGFEA
                     RTRASVIQVTNLGITVKDSPQNTLVFVTRLDSGDPVAGATVSIVRMDNSTFWRGTTGA
                     DGVAMAPETPLRDPANIWRRQLDFVVTAEKDGDIAYVGSDWNEGIMPWDFGTGVNLQE
                     REPLLRGSVFTDRGVYRLGEEIHFKAILRHNSPAGVRLLPEGTAVLMTVRNAQNRLVD
                     ERVVRLTPWSSAEWTMTLPADGALGNYSLRALLESDRPKPKTPDQRRPGVTPSPEDDQ
                     FVPYEKTVNGSFLVAAYRRPDFRVDVSLSGANPVAGDQVKGVVTARYLYGASMGVRPV
                     TWRYSKSPGFTAPSAITEKFGDDRWVFVGWSDREQPFETGDIRREETTLQATGDLPLS
                     LDTRR"
     gene            2752..>3929
                     /locus_tag="AC16CY_00615"
     CDS             2752..>3929
                     /locus_tag="AC16CY_00615"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3352303.1"
                     /translation="MTKRLSPSTPSAAVLCAALTIFVALTAGGTDALQQAPAPPGGRG
                     QAAAPAPPGQDAARGQGEGGRGQGGRGRGAPAEVDTLGAGPWDFPAGRGGRVKVSVVT
                     KGVDHPWGIAFLPDGDMLVTERPGRLRVIRKGVLDPTPIGPMPEMLATGLGGLLDVSL
                     HPQFAKNRLIYLTYSKPGPGQGNATTAVLRARWDGGATLADVKDILVADAYHGGPGSP
                     QGLGPASGSYGSRLAWDRAGFLYVSLGDRNYPPAAQDPASHIGKILRIRDDGRVPADN
                     PFVSRPGYKPEIYSLGHRNPLGLAIHPVTGDLWSTEQGPQGGDELNKIEMGKNYGWPR
                     VSLGRNYDGTRVGEGFTALGLEEPVVYWVPAIAVSGLSFYDGDKFPAWKGNAFVGAMR
                     A"
BASE COUNT          626 a         1316 c         1363 g          624 t
ORIGIN      
        1 cacggcgcgt gtcgagcgac agcgggagat cgcccgtcgc ctggagcgtc gtctcttcgc
       61 ggcggatgtc tccggtctcg aagggctgct cgcggtcgga ccagccgacg aacacccacc
      121 tgtcgtcgcc gaacttctcg gtgatggccg agggcgccgt gaagcccggc gacttcgagt
      181 acctccaggt caccggccgc acgcccatcg acgcgccgta cagataccgg gccgtgacga
      241 cgcccttcac ctgatccccg gcgacgggat tggcgccgct cagcgacacg tccacacgga
      301 agtccggccg ccggtacgcc gccacgagaa acgacccgtt caccgtcttc tcgtacggca
      361 cgaactgatc gtcctcggga ctgggcgtga cgccgggccg ccgttggtct ggcgtttttg
      421 gcttggggcg atcgctttcg agaagggcgc gcagcgagta gttgccgagc gctccatccg
      481 cgggcagcgt catcgtccac tcggcgctgc tccacggcgt gaggcgcacg acacgttcgt
      541 caaccagccg gttctgcgcg ttgcgcacgg tcatcagcac ggccgtaccc tcggggagca
      601 gccggacgcc ggcgggagag ttgtgtcgca ggatcgcctt gaagtggatc tcttcgccga
      661 gccggtacac gccgcggtcg gtgaagacgc tcccgcgcag cagcggctcc cgttcctgca
      721 ggttgacacc ggtgccgaag tcccacggca tgatgccttc gttccagtcg ctgccgacgt
      781 aggcgatgtc gccgtccttt tcggcagtga ccacgaagtc gagctgtcgt cgccagatgt
      841 tggccgggtc acgaagcggc gtctccggcg ccatggcgac gccgtccgca cccgtggttc
      901 cgcgccagaa cgtgctgttg tccatccgga ctattgacac ggtcgcgccg gcaacgggat
      961 cgcccgagtc cagccgcgtc acgaacacca gcgtgttctg cgggctgtcc ttcacggtga
     1021 tcccgaggtt ggtgacctgg atgacggacg cgcgcgtacg cgcctcgaag ccgacgcggc
     1081 gcgtgcgcgg caggagctcg ccttcccgca cggccgtcca gacaagaccg gttccgccgg
     1141 gacgcagggc gccggacaag tcgagcccgt gcgactgcac acgatccggc gtgacgccga
     1201 gccggcggtt gactccgtcg ccggccggcg gcgagttgaa gcggtctttc tggagctcga
     1261 ggagcctcgg catcagctgc gatggctcga gcgccgatgc ccattgccgg acgtcgagca
     1321 ggttccgcgc gtagaacggg agctgtgccc cgccgtcttt ctcccagacg ccgtggccgt
     1381 cgccaaagga cgtaaacgcg cgcatgtgcc agttgtccac gatccccagc catgggtagc
     1441 cgagcgtctg accgtcggcg gccctgagcg aggcggcggc gacgacgacg tacttccgat
     1501 tgggtggctg cgcgttgaac ccggcgtctt cgagcgtcag aaagttgctg gcctccggcg
     1561 cgtacacggg acgcggccgc ggagtggacg gtttttgaac gggctgttcg ccggaggtaa
     1621 tgtccgtggc gcgcaacgcg gcggcaaagt cagccaccct gaccggtgcg cgcactcgca
     1681 tcggattcca ggcgtcgccg tcgcactcgg aggtgcagcg aaagtcctcg atgaagaacg
     1741 cggactcggc cctgatcgtg aacgactgtt ccccgggcgg tgtggccggg ccggccggcg
     1801 acggcaaagc cgcgtcgagc gcgagcctga cccagctctc tggccggacg agggaggtgg
     1861 tctcgaacgc gacgagatcc ggcgaggccg ggaagcgctt ctgatcccaa tcgctcgtca
     1921 ggcgaagacc gacggccccg ccgcctgccg cgacgagtcg cgtcgccgcc accttgtccg
     1981 caaaggactt gagccctgac ggatcgagcg cacgaagccg cgtctcctcc tcaggagtga
     2041 acgccggtgg attccagtcg tgcctggcaa gcgacgcagt gagatgcgcg gcgacatcct
     2101 gtgccctcac cggctgattg aaccggagca gcgccaccat cgggctcccg atcgttccgt
     2161 cgcggcggta ccaattggtc tgcagcagcc gcaccgtcgg cgtcgtgaag cggaacgtct
     2221 cggcgcgcgc cagcgttcgg ccgctgaccg cggtcgcgcc gtcgatcgtc acctcgtagg
     2281 aggtcgcgta cggcagcggc tgcttcggat ccggcgtgaa gatgagaatc gtcgtcccgg
     2341 accaccggaa cgtcccgctg atcgccgggc tgattcgaac aaacggtgca tccaccttcg
     2401 ccgggatccg tcccagcgcg accatcggct ccgagaagac gatccggatc tcgttggctt
     2461 gcgcgagact atcgagccgt ccctgcgggc cggagttgac ggtcgtgagc ggggcttcct
     2521 gtgcggcagt gatggtgatg aggacggaga ccaggaacac caggatggat ccgacacgcg
     2581 acatcgcgac cctcgtcatc gggggagagt cagggggtac cggattggac accatatcgg
     2641 cacgcgtgct cctcgtgagg cgtgtgtctt tcgggccgcg ctgccctata ctgacgctct
     2701 tcaaccaacg tagtgtccgg cttcagccgg accgagcgaa ggagatcgac gttgacaaag
     2761 cgattatccc cctcgacacc gtcggccgcc gtgttgtgtg ccgcgttgac gattttcgtg
     2821 gcgctgactg ccggcggcac cgacgcgctg cagcaggcgc cggcgccgcc agggggccgt
     2881 ggtcaggcgg ccgctcccgc gccaccggga caagacgcag ccagggggca gggggaaggg
     2941 ggcagggggc aagggggaag gggacgaggt gctccggccg aggtcgatac cctgggagca
     3001 gggccgtggg acttccccgc cggcaggggc ggccgcgtca aggtgagcgt cgtgacgaag
     3061 ggggtcgatc acccgtgggg catcgcgttc ctcccggatg gagacatgct cgtcaccgaa
     3121 cggcccggcc gtctgcgcgt catccggaag ggcgtgctcg atccgacgcc gatcggtccg
     3181 atgccggaga tgctcgcaac gggacttggc ggtcttctcg acgtgtcgct gcatccgcag
     3241 ttcgccaaga accgcctgat ttacctgacc tactccaagc cgggcccggg tcaggggaac
     3301 gccaccacgg ccgtcctgcg cgcgcgatgg gatggcggcg cgacgctcgc cgacgtcaag
     3361 gacatcctcg tcgccgacgc gtatcacggt ggaccgggct cgccgcaagg cctgggtcct
     3421 gccagcggca gctacggctc gcggctggcg tgggaccgtg cgggcttcct ctacgtgtcg
     3481 ctcggcgatc gcaactaccc gccggcggcc caggacccgg cgtcgcacat cgggaagatt
     3541 ctccgcatac gcgacgacgg ccgcgtgccg gccgacaacc cgttcgtgag cagaccgggt
     3601 tacaagccgg agatctactc gctcggccac cgcaatccgc tgggcctcgc catccacccg
     3661 gtgaccggcg acctgtggtc caccgaacag gggcctcaag gaggcgatga gctgaacaag
     3721 atcgaaatgg gcaagaacta cggatggccg cgagtgtcgc tcggccgcaa ctacgacggc
     3781 acgcgcgtgg gcgaaggctt cacggcgctc ggcctcgaag agccggtcgt ctactgggtt
     3841 ccggcaattg ccgtgtccgg cttgtccttc tacgacggcg acaagttccc tgcatggaag
     3901 ggcaacgcct tcgtcggcgc gatgcgcgc
//
LOCUS       DBNGSH010000030         1588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2888791, whole genome shotgun sequence.
ACCESSION   DBNGSH010000030 DBNGSH010000000
VERSION     DBNGSH010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2888791"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="AC16CY_00620"
     CDS             <1..1569
                     /locus_tag="AC16CY_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352304.1"
                     /translation="PRQEQLEQLLAASMERTGTTRAIPLGVQTTIVRGQDPKRLEAIV
                     IVRTSHPAAQGALIGLAVRTGSEIVAAQTGRLDEAQRVGGVLSHVSTIAVPPGRHQLR
                     VAVVDAQGQEGSVNHTFAADLHAAGKVLLSDLIVGQPAGTGPVRPAIDVSNERPVSAL
                     LELYSFEPVKEPPAVTMTISALGNPSVHREAVAQVERTEVEGIQRAVSSIDVRGLPAG
                     EYLVRASVRSGGGVSMLDRHVVVSGRATSASATAPSASDVPGGIPLVVRYASRYAEGY
                     LKELSNVLMDETYRQEQRVLTRLNVRTLEAEVLFVSLPAPVGPTAFRDVFSVDGRQIR
                     DRDDRLQRLFATPQPSAFDQAQRILQESAKHNLGAAVRTLNLPTAPLLFLRAEHLARM
                     QWTTAGDQQVAGRRHNRVRFRELQTPTLLRTPRGGDVAVTGEFLIDVETGAVSRAELS
                     MVVRDDSRRSATPELDARIGVEFRRHDAWGQLVPGTMTERYLLQSGEENTARAEYANY
                     RRFRVETSDDVSPK"
BASE COUNT          242 a          548 c          576 g          222 t
ORIGIN      
        1 ccgcggcagg agcagctcga gcagctcctc gcagcgtcga tggagcgcac cgggacgacc
       61 cgcgccattc cgctcggcgt ccagacgacg atcgtgcgcg gccaggaccc gaagcggctc
      121 gaggcgatcg tgatcgtgag gacgagccat cccgccgcgc agggcgcgtt gattgggctc
      181 gcggtgcgca ctggctcaga aattgtcgcg gcgcaaacgg gccgcctcga cgaggcgcag
      241 cgagtggggg gcgtcctgtc gcacgtctcg accatcgccg tcccgccggg caggcaccaa
      301 ctgcgcgtcg ccgtcgtcga tgcgcagggg caggaaggca gcgtcaacca tacgttcgcg
      361 gcagatctgc acgcggcggg gaaggtactc ctgagcgacc tgatcgtcgg ccagccggcc
      421 ggcaccggtc cggttcggcc cgcgatcgac gtctcgaacg agcggcccgt cagtgccctg
      481 ctcgagctct actccttcga gcccgtgaag gagccgccgg ccgtgacgat gacgatctcc
      541 gcgctcggca atccttctgt gcatcgagag gccgtggccc aggtggagcg gaccgaggtc
      601 gaaggcatcc aacgcgccgt cagctcgatc gacgtccgcg gcttgccggc cggcgagtat
      661 ctggtgcgcg ccagcgtgcg cagcggtggc ggcgtctcga tgctcgaccg ccacgtcgtc
      721 gttagtggca gggcgacgtc ggcgtccgcg acggcgccat ctgcctccga cgttcccggc
      781 gggattcctc tggtggtacg ttacgcgtcc cggtacgcgg aaggctacct caaggagttg
      841 tccaacgtgc tgatggacga gacgtaccgc caggagcagc gggtcctgac gcggctgaac
      901 gtgcggacgc tcgaggcgga ggtgctgttc gtttcgctgc cggcgccggt gggcccgacg
      961 gcattcagag acgtgttctc cgtggacggc cggcagatac gcgaccggga cgatcggctg
     1021 cagcggctgt tcgccacgcc ccaaccgtcc gcgttcgacc aggcgcagcg catcctgcag
     1081 gaaagcgcga agcacaacct gggggccgcc gtccggacgc tgaacctgcc gacggcgccg
     1141 ctgctcttcc tgcgcgccga gcatctggcg cgcatgcagt ggaccacggc cggcgatcag
     1201 caggtggcgg gccggcgcca caaccgcgtc cggttccgcg agctgcagac gcccacgctg
     1261 ctgcggacgc cgcggggcgg tgacgtggcg gtgaccggcg agttcctgat cgacgtcgag
     1321 acaggagccg tttcgcgcgc ggagctgtcg atggtcgtgc gcgacgacag ccgccgcagc
     1381 gcgacgccgg agctcgacgc ccggatcggc gtggagttcc gccggcacga cgcgtggggc
     1441 cagctcgtcc cggggacgat gaccgagcgg tacctgctcc agagcggcga ggagaacacc
     1501 gcccgcgccg agtacgcgaa ctatcgccgc ttccgcgtcg agaccagcga cgacgtgagc
     1561 ccgaagtagg ccgcgccgcc taccacgc
//
LOCUS       DBNGSH010000031         2414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2216, whole genome shotgun sequence.
ACCESSION   DBNGSH010000031 DBNGSH010000000
VERSION     DBNGSH010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2216"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="AC16CY_00625"
     CDS             complement(<1..590)
                     /locus_tag="AC16CY_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3352305.1"
                     /translation="MKYRYTKYTGNLLDEIDLEDLVSKLSDLLLSSGFSNPWSGPQEG
                     DEDRTMQTLHDAILEALFTGGVLSEDALQQLLGEPADGDQEQARQQLEELIQQIIDKM
                     MEQGFISSPPDLEGEREHRRSGGGVGDEAGPVKFEVTDKGLDFLGYRALRDLLGSIGR
                     SDTGRHDTRHLSTGIEAIAAPKPYEFGDTFNIDPSST"
     gene            complement(591..2102)
                     /locus_tag="AC16CY_00630"
     CDS             complement(591..2102)
                     /locus_tag="AC16CY_00630"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR3352306.1"
                     /translation="MSRSAISTLGALREAVERGDVVRRPVRQEVRDNLICKLRTGQRL
                     FPGIIGYDDSVVPQLVNALLSQHNFILLGLRGQAKTRLLRALTTLLDEEIPVVPGCEV
                     NDDPLAPLCGACRARVADEGDDLAISWLHRDFRYVEKLATPDVTIADMVGDIDPIKAA
                     RSGLQLSSELTMHYGLLPRANRGIFAINELPDLAGKIQVGLFNILQEGDVQIKGYPIR
                     LPLDVLMAFTANPEDYTARGKIITPLKDRIGSEIRTHYPAGRADAQAITAQEAWLARN
                     GVAPKADIPTFVAEIVEEIAFQARQDRRIDKRSGVSQRLPISAMENVVSNAERRAIAS
                     GEAVAVARVTDVYSALPSITGKFEMEYEGELKGAEAVARDLIRASVATVAAGYLSHLD
                     TRQVVEWFDLGGSLQLSDTMSAAEVLSHARQVQGLVELSCSAGVPANPSEPMLAAGVD
                     FLLEGLYATKKISRSDERGYHGAEPALRKPAREISLDEEPRGPSGGGRKKYYN"
     gene            2193..>2414
                     /locus_tag="AC16CY_00635"
     CDS             2193..>2414
                     /locus_tag="AC16CY_00635"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3352307.1"
                     /translation="MLSCRVPFFGDSSRPRVFAHRGGCAIGPENTMAAFDLGLAAGAD
                     GLEIDVHLSSDGVVVVCHDATLDRTTDASG"
BASE COUNT          351 a          836 c          788 g          439 t
ORIGIN      
        1 gtactgctcg ggtcgatgtt gaacgtgtct ccgaactcgt acggcttcgg cgccgcgatc
       61 gcttcgatgc ccgtgctcag gtggcgcgtg tcgtggcgcc cggtatcgct gcggccgatg
      121 gagcccagca ggtcccgcag cgccctgtag cccaggaagt cgagcccctt gtcggtgacc
      181 tcgaacttga cgggccccgc ctcgtctcca acgcccccgc ccgatcggcg gtgctcccgc
      241 tcgccctcga gatccggcgg actggagatg aatccctgct ccatcatctt gtcgatgatc
      301 tgctggatca gttcctcgag ctgctgccgc gcctgctcct ggtcgccgtc ggccggctcg
      361 cccagcagct gctgcagggc atcctccgag agcactccgc cggtgaacag cgcctcgagg
      421 atggcgtcgt gcagcgtctg catcgtgcgg tcctcgtcgc cctcctgcgg gccggaccac
      481 gggttgctga atccgctcga gagcagcaga tccgaaagct tcgagacgag atcctcgagg
      541 tcgatctcgt ccagcaggtt cccggtgtat ttggtgtagc gatatttcat ctagttgtaa
      601 tacttcttcc gcccgccgcc gctcggccct ctcggctcct cgtcgaggct gatctcgcgc
      661 gccggtttgc gcaacgccgg ctcggcgccg tgatacccgc gctcgtcgct ccggctgatc
      721 ttcttcgtcg catagaggcc ttcaaggaga aagtctacgc ccgcagcgag catcggctcc
      781 gatgggttcg ccggaacgcc ggccgagcac gagagctcga ccagcccctg cacctgccgc
      841 gcgtgcgaca gcacctcggc ggcgctcatc gtgtcgctca gctgcaggct tccgccgagg
      901 tcgaaccact cgacgacctg tctggtgtcg aggtgcgaca aatacccggc ggcaacggtc
      961 gcaaccgagg cccgaatcag gtcgcgcgcg acagcctcgg ctcccttcaa ctcgccctcg
     1021 tactccatct cgaacttccc ggtgatcgac ggcagcgcgg agtacacgtc ggtcacgcgc
     1081 gcaacggcga ccgcttcgcc ggaggcgatg gcccgccgct cggcgttcga cacgacgttc
     1141 tccatcgccg aaatcggcag gcgctggctc acccccgatc gcttgtcgat ccggcggtcc
     1201 tgccgtgcct gaaacgcgat ctcctcgacg atctcggcga cgaacgtggg gatgtcggct
     1261 ttcggcgcca ccccgttgcg cgcgagccac gcttcctgcg ccgtgatggc ctgcgcgtcg
     1321 gcgcgtccgg ccggatagtg cgtcctgatc tcggacccga tccgatcctt gagcggcgtg
     1381 atgatcttgc cgcgcgccgt gtagtcctcg gggttcgccg tgaacgccat cagcacgtcg
     1441 aggggcaggc gaatcgggta gcccttgatc tggacgtcgc cctcctgaag gatgttgaag
     1501 aggccgacct ggatcttccc ggccaggtcc ggcagctcgt tgatggcgaa gatgccgcgg
     1561 ttggcccggg gcagcagccc gtagtgcatg gtcagctcgc tcgagagctg cagtcccgat
     1621 cgcgccgcct tgatcggatc gatgtcgccc accatgtcgg cgatggtgac gtcgggcgtg
     1681 gccagcttct cgacgtacct gaaatcgcga tgcagccacg agatggccag gtcgtctccc
     1741 tcgtccgcca cgcgggcacg gcacgcgccg cagagcggcg ccagcgggtc gtcgttgacc
     1801 tcgcacccgg gcacgacggg aatctcttcg tcgagcagcg tcgtcagcgc tcgaaggagg
     1861 cgcgtcttgg cctggccgcg caggccgagc aggatgaagt tgtgctgcga gaggagcgcg
     1921 ttcaccagct gcggcacgac gctgtcgtcg tagccgatga tgccggggaa gagcctctgg
     1981 cccgtccgca gtttgcaaat caggttgtcc cggacttcct gccgcacggg acggcgcacg
     2041 acgtcgccgc gctccaccgc ctcgcgcagc gcaccgagag tggaaatggc cgatctcgac
     2101 atggccagat cctatcaggc ctcagggccc agggctcagg cctcagggcc cagggctcag
     2161 gcctcagcgc agggctcagg cagaaccctt cgatgctatc gtgccgtgtg cctttcttcg
     2221 gggattcgtc ccggcctcgt gtgttcgctc atcgcggcgg atgcgcgatc ggtcccgaga
     2281 acacgatggc ggccttcgac ctgggccttg ccgcgggcgc cgacggactg gagatcgacg
     2341 tgcacctgtc gagcgacggc gtggtcgtcg tctgccatga cgcgacgctc gatcggacga
     2401 cggacgcctc gggg
//
LOCUS       DBNGSH010000032         2744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2209668, whole genome shotgun sequence.
ACCESSION   DBNGSH010000032 DBNGSH010000000
VERSION     DBNGSH010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2209668"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="AC16CY_00640"
     CDS             complement(<1..1073)
                     /locus_tag="AC16CY_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3352308.1"
                     /translation="MIGALPRIDLGSLKFADPLYLWLLVVPAVLLVVCAWRLIRRRGD
                     LVRLTSRRALPVRERFGLLGDLAFWAAVLMASALCVVALARPQARVSSVRRASADVVI
                     LQDASASMYVTDVRPDRWRRSVQFLRTFAETLSWQGDRVALALFAQLAAPQVRLTKDP
                     NALFFFIDHLGDKSPFRLENLTSWDTNIEEGIRWGLRLVEKDEEVFGKNGNPKAFLVI
                     SDGQSWSGTVANALRTAHDRNIPVHVVGIGTTTGGMIPEPARPDGTRPPPVIRSVLDR
                     SSLVQIAVAGGGEYFEIGDEPDRIVAFRIVDTLRRRAEVVKEVESFDELYGRFLMAAA
                     ILLVLGTAFLRRRSELGWQAAAAL"
     gene            complement(1070..1759)
                     /locus_tag="AC16CY_00645"
     CDS             complement(1070..1759)
                     /locus_tag="AC16CY_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352309.1"
                     /translation="MRSIVASVIGAVVLGVAGFAFLGVYRLDAHMADAGQRLSTLQYE
                     AASASLAEAERYSGSARFVPWIGSDAREEIRARQAAVQYWQRKYDEVIPVGAEPVAAV
                     DENNVELQLVVANAAYRVKQAQFKDRQSTIKALEEAAAGYLTVLKNNTWHPDAAFNYE
                     YIVRLRDEAGKGRQPPPRENEEESDLGESGAPTPATSQEGFEIYIPLEQGEKNPSGGE
                     AGKAAPKERKG"
     gene            complement(1845..>2744)
                     /locus_tag="AC16CY_00650"
     CDS             complement(1845..>2744)
                     /locus_tag="AC16CY_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3352310.1"
                     /translation="LPALLPVMRRSPLTFVRHLPLLLFAAGIPLFAIALADPLTGFSR
                     EEVSYPGRRIALLVDGSTSMVIRFDSTVKRPGESTFFTAVAAAEQFIRRRMTGPYRDL
                     VALIQFGNFAYVVTPFTNDYENILLSTRLIGDPAEWGRFPDWGTTIIEGIDQATQLFR
                     TFDFTNASGNLMIVFTDGRDSQLNRQKADLDRIVGEARTWKIPVYMMRTAYNRREGEI
                     LQDKIWKETVEQTGGRFYAAYDDESIERALREIDRLSPGRIETREYSVRRPRFAGWTL
                     IAVGLWLTGALLKLNFGVFRTFP"
BASE COUNT          461 a          958 c          881 g          444 t
ORIGIN      
        1 agcgcggcgg ccgcctgcca tcccagctcg gatcgcctgc gcagaaacgc ggtgccgagc
       61 acgagcagaa tcgcggccgc catcaggaaa cggccgtaga gctcgtcgaa gctctcgacc
      121 tccttcacga cctcggcgcg gcggcgcagc gtgtccacga ttctgaacgc gacgatgcga
      181 tccggctcgt cgccgatttc gaaatactcg ccgccgcccg cgacggcgat ctgcacgagc
      241 gacgatcgat cgagcacaga ccggatcacc ggaggcggcc gcgtgccgtc gggccgcgcc
      301 ggctcgggaa tcattcctcc cgtcgtcgtg ccgatgccga cgacgtgcac cggaatgttg
      361 cggtcgtgcg ccgtccgcag cgcgttggcc acggttccgc tccaggactg cccgtcggag
      421 atcacgagga acgctttcgg attgccgttc ttcccgaaca cctcctcgtc cttctcgacg
      481 aggcgcagcc cccacctgat gccctcctcg atgttcgtat cccagctcgt gaggttctcg
      541 aggcggaacg gcgacttgtc gccgaggtga tcgatgaaga agaagagcgc gttcggatcc
      601 ttggtcagtc tcacctgggg cgcggccagc tgcgcgaaga gcgccagcgc cacgcggtct
      661 ccctgccagc tgagcgtctc ggcgaacgtg cgcaagaact gcacggaccg tcgccagcga
      721 tcgggccgca cgtcggtgac atacatcgac gcggacgcat cctggaggat cacgacatcc
      781 gcgctcgccc tgcgcaccga cgacacgcgg gcctgcgggc gggccagcgc cacgacgcac
      841 agcgccgacg ccatgagcac ggccgcccag aacgccaggt cgccgagcag gccgaatcgc
      901 tcccgcaccg ggagcgcacg tctcgacgtc agtcgaacga gatctccgcg ccggcggatc
      961 agccgccagg cgcagacgac gagcagcacc gccggaacga cgagcagcca gaggtacagc
     1021 gggtcggcga acttcagcga gccgaggtcg atcctcggaa gcgcgccgat caccccttgc
     1081 gctccttcgg ggcggccttc cccgcctcgc cgccgctcgg gttcttctca ccctgctcga
     1141 gcgggatgta aatctcgaag ccttcctggc tcgtcgccgg cgtgggcgcg cccgattcac
     1201 cgaggtccga ttcctcctcg ttctcgcgag gcggtggctg gcgtcccttg ccggcttcgt
     1261 cgcggaggcg gacgatgtac tcgtagttga acgcggcgtc gggatgccag gtgttgttct
     1321 tcaggacggt gaggtagccg gccgcagcct cctcgagcgc cttgatcgtc gactggcggt
     1381 ccttgaactg tgcctgcttg acgcgatagg cggcattggc gacgacgagc tgcagctcca
     1441 cgttgttttc gtccacggcg gccaccggct cggcgccgac ggggatcacc tcgtcgtact
     1501 tccgctgcca gtactgcacg gcggcctggc gcgcccggat ttcctcgcgc gcgtcgctcc
     1561 cgatccaggg cacgaagcgc gcggagccgc tgtagcgctc ggcctcggcg agactcgcgc
     1621 ttgccgcctc gtactgcagc gtggacaacc gctgtccggc gtccgccatg tgcgcgtcga
     1681 gccggtacac cccaaggaag gcgaacccgg cgactcccaa aacgacggcg ccgatcaccg
     1741 acgcgacaat gcttcgcata tattgagtct ccgccacaat caggcgggtc taaagacccg
     1801 cctctacttc tccccgcccc ctgtcccctg ccccctgcca cccatcacgg aaacgtcctg
     1861 aatactccaa agttcagctt caacagtgcg cccgtcagcc acaggcccac ggcgatgagc
     1921 gtccacccgg cgaaacgcgg acgccgcacg ctgtactcgc gcgtctcaat ccgccccggc
     1981 gacaggcgat cgatctcccg caacgctctc tcgatcgatt cgtcgtcgta ggccgcgtag
     2041 aatcgtccgc ccgtctgctc gacagtctcc ttccagatct tgtcctgaag gatctcgcct
     2101 tcgcgccggt tgtaggcggt gcgcatcatg tacacgggga tcttccaggt ccgggcctcg
     2161 ccgacgatgc gatccaggtc cgccttctgc cggttcagct gcgagtcgcg cccgtcggtg
     2221 aagacgatca tcaggttgcc ggacgcgttc gtgaagtcga aggtgcggaa caactgcgtg
     2281 gcctggtcga tcccttcgat gatggtggtg ccccagtcgg ggaagcggcc ccattcggcc
     2341 gggtcgccga tgaggcgcgt gctcaggaga atgttctcgt agtcgttggt gaacggcgtg
     2401 acgacgtagg cgaagttgcc gaactgaatc agcgcgacca ggtcgcggta gggacccgtc
     2461 atgcgccgcc tgatgaactg ctcggccgcg gcaaccgcgg tgaagaacgt cgattcgccc
     2521 ggacgcttca cggtcgagtc gaaccggatc accatgctcg tcgagccgtc aacgagcaag
     2581 gcgatgcgcc gccccggata cgacacttcc tcgcgggaaa acccggtgag cggatccgcc
     2641 agagcgatgg cgaacagcgg gatcccggcg gcaaagagca gcagcggaag atgcctgacg
     2701 aaggtcagcg gcgaccggcg catgacgggc aggagcgcgg gcag
//
LOCUS       DBNGSH010000033         2429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4247234, whole genome shotgun sequence.
ACCESSION   DBNGSH010000033 DBNGSH010000000
VERSION     DBNGSH010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4247234"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="AC16CY_00655"
     CDS             complement(<1..526)
                     /locus_tag="AC16CY_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3352311.1"
                     /translation="MRIARRGFVAACAVAAIPVSQSAAQTKDAVTFTKDIAPILQRSC
                     QQCHRPGSVAPMSLLTYEEARPYARAMKARTALRDRRGAMPPWYIEKNVGIQQFKDDF
                     SLSEEDIAKVAAWADNGAPQGNPADMPPPLTFPDNKAWRIGTPDLIVVSPPVEMKGVA
                     PDWFGAIGTSPTGLT"
     gene            complement(613..1203)
                     /locus_tag="AC16CY_00660"
     CDS             complement(613..1203)
                     /locus_tag="AC16CY_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3352312.1"
                     /translation="MVRVVPVTIINVGYRSTNYWLVSAGTSRLLVDIGWPGTLGTMKA
                     NLKRMGVPLHEIRYALATHYHPDHAGLAEELKREGVRLLVLDVQVDAIPLMKTWTKPR
                     DNYVEITGDGNILISFEQSRDLLGQMGIAGEILHTPGHSDHCVSLLLDDGSVFTGDLP
                     PEGAAFDNPVALATWSRLRERGATRVYPAHGPTRSM"
     gene            complement(1229..1747)
                     /locus_tag="AC16CY_00665"
     CDS             complement(1229..1747)
                     /locus_tag="AC16CY_00665"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3352313.1"
                     /translation="MNGTITIRPERREDSAAVIAINDRAFGGTGESGLIDAVKQSGRP
                     VISLIAASDDNPVGHIFFSPIQIQSEGPLIATLALAPMAVLPPFQRRGIGTLLVESGL
                     RECARQGCQVVVVVGHPSFYRRFGFGPANEMGLRSVYSSAGDAFMALELSKGVLAGRT
                     GAVEYPVEFANV"
     gene            complement(1755..>2429)
                     /locus_tag="AC16CY_00670"
     CDS             complement(1755..>2429)
                     /locus_tag="AC16CY_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352314.1"
                     /translation="NRTNSGGEVLAERPVDAFPADPTSPPVAAFWDLETNVRLPLTPW
                     AAALKKERMASDMKDNPDANCMPMGITQFHMQPQPRKIVQTPKLIVILYESNYGLRYI
                     YTDGRRLPKQGEPQPWWYGYSVGRWEGDTLVVTTNNLRGAEEGPYDGWLDVRGTPYSG
                     QATFTERFRRPTFGKLEIDFTVEDAKAFTKPFTMRINQRVLVDEEPIEFVCNENQLFR
                     RRVKVD"
BASE COUNT          410 a          768 c          792 g          459 t
ORIGIN      
        1 cggtcaggcc cgtcggcgac gtcccgatcg cgccgaacca gtcgggcgcg acgcccttca
       61 tctcgaccgg cggcgagacg acgatcaggt ccggcgtgcc gatgcgccag gccttgttgt
      121 cggggaaggt gaggggcggc ggcatgtccg ccgggttgcc ctgcggcgcg ccgttgtcgg
      181 cccaggcggc gaccttggcg atgtcctctt cgctcagcga gaaatcgtcc ttgaactgct
      241 gaatgccgac gttcttctcg atgtaccagg gcggcatcgc cccgcgcctg tcgcggagcg
      301 cggtcctggc cttcatggcc cgcgcgtagg gccgcgcctc ttcatacgtg agcagcgaca
      361 tcggcgcgac cgagccgggg cgatggcact gctggcagct gcgctgcagg atcggcgcga
      421 tgtctttcgt gaaggtcacc gcgtctttcg tctgcgcagc ggactgcgac acggggatgg
      481 cggcgaccgc gcacgcggcc acgaagccgc gccgtgcgat gcgcatccag gattcacgtc
      541 gtcgggtggg cgtgtatttc atagagtctc tctatcaggc tccgcggccc tgggcgcgcg
      601 ctcgatgtca gtctacatcg accttgtggg gccatgcgca ggatacaccc gcgtcgcgcc
      661 ccgttctcgc aagcggctcc acgttgccag agcaacggga ttgtcgaacg ccgcgccctc
      721 cggtgggaga tcgccggtga agactgaacc atcgtcgagc aggagcgaga cacaatgatc
      781 ggagtgaccg ggcgtatgaa ggatttctcc agcgattccc atctggccca ggagatcccg
      841 gctctgctcg aatgagatga ggatgttgcc atcaccggta atctcgacgt agttgtcccg
      901 tggctttgtc cacgtcttca tgagggggat cgcgtcaacc tgaacatcga ggacgagaag
      961 gcgaacgccc tctcgcttca attcctcagc cagaccggca tgatcggggt gatagtgggt
     1021 ggcgagtgcg tagcggattt cgtggagggg cacccccatc cgcttgaggt tcgccttcat
     1081 ggttccgagt gtgccgggcc agccgatgtc gacgagcaac cgggacgtgc cagcgctcac
     1141 gagccagtag ttggtcgacc ggtacccgac gttgatgatc gtcaccggca ccactctgac
     1201 catagttcat acagctcgga agagatgcct agacattcgc gaattccacc gggtattcga
     1261 cggcccccgt gcgcccggcg agcacccctt tcgataactc gagggccatg aaggcgtcgc
     1321 ccgctgaaga gtagaccgac ctcaagccca tctcgtttgc gggaccaaat ccgaaacggc
     1381 gatagaagga cggatgtccg acgaccacga cgacctggca accctgcctc gcacactccc
     1441 tgaggcccga ttcgaccaga agcgttccga tgcccctccg ttgaaatggc gggaggaccg
     1501 ccattggtgc aagcgcaagc gtggcaatca ggggaccttc cgactgaatc tggatcgggc
     1561 tgaagaagat gtgccctacg ggattgtcgt cggaagccgc gatgagagag atcacaggcc
     1621 ggccggattg cttgaccgcg tcgatgagcc cggactcccc ggtgcctcca aatgcgcggt
     1681 cgttgatggc gatcacggca gctgagtctt cgcgccgctc cggccgaatg gtgatcgttc
     1741 cgttcaaggc gacgtcagtc cactttcacc cgccgcctga acagctggtt ctcgttgcag
     1801 acgaactcga tcggctcttc gtccaccagc acgcgctggt tgatgcgcat ggtaaacggc
     1861 ttggtaaacg ccttggcgtc ctcgacggta aagtcgatct ccagcttgcc gaacgtcggg
     1921 cggcggaagc gctcggtgaa cgtcgcctgg ccgctgtacg gcgtcccgcg cacgtcgagc
     1981 cacccgtcgt aggggccctc ttccgcgccg cggagattat tggtggtgac gaccagcgtg
     2041 tcgccctccc agcgtcccac cgagtagccg taccaccacg gctgcggctc tccctgcttc
     2101 ggaagcctcc gcccgtcggt gtagatgtag cgcaggccgt agttggattc gtacagaatc
     2161 acgatcagct ttggcgtctg gacgatcttc ctcggctgcg gctgcatgtg aaactgggtg
     2221 atccccatcg gcatgcagtt ggcgtcgggg ttgtccttca tgtcgctcgc catccgctct
     2281 ttcttcaggg ccgcggccca gggggtgagc ggcaggcgaa cgttcgtctc gagatcccag
     2341 aacgcggcta cgggaggtga cgtcgggtcc gcggggaagg cgtccacggg ccgctcggcg
     2401 aggacttcgc ctcccgagtt ggtgcggtt
//
LOCUS       DBNGSH010000034         5273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4077744, whole genome shotgun sequence.
ACCESSION   DBNGSH010000034 DBNGSH010000000
VERSION     DBNGSH010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4077744"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1084
                     /locus_tag="AC16CY_00675"
     CDS             155..1084
                     /locus_tag="AC16CY_00675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3352315.1"
                     /translation="MRSRPPASSLGAFAYYGGLRLLGLTSLRRRQQDGGLILCYHNVV
                     PGDGGREGDPGLHMSCVRFERHMRWLALHYNVVPLHEFVQRVEMGASLQSVAAITFDD
                     AYAGVFAHAAPLLEALELPATVFVVADAPGRSDLFWWDRPDVVTFAARPDTRQEWLTQ
                     LQGDQAAIVSSLGASAVAPLPSTHYAADWATIRAHAGRQLDIGVHSATHRSLTTLSDA
                     DLEREIVTSRAVIYHATGVWPQCFSYPYGLWDGRVRDAVRAAGYRAACTTDPEFYSRP
                     ADVCALPRLNVPAGISDGAFEAWTAGFRGSKAS"
     gene            1081..2253
                     /locus_tag="AC16CY_00680"
     CDS             1081..2253
                     /locus_tag="AC16CY_00680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3352316.1"
                     /translation="MMNRHPRVAGLRLAHLIESSGPGGAERVVAELATSLQAGGADNV
                     VFLPADGEDWLRRQLEGSGVAVEYFRIERPFSVACARSLALGFQKHRIDLAHSHEFSM
                     AFYGAWAARLAGVPHVITMHGGRYYAGRLRRRLALRAAMALSARTVAVSGRLALAIGR
                     DLGIRATRILTIPNGVRYLPPERVTLRDDLRLGPDDRLIVAVGNLYPVKGHQYLIDAA
                     ASIIDRHPTLHIAIAGRGDREAALRSQARDRGLADRVHLLGLRADIPAILAAADAFVL
                     PSLSEGLPLALIEAMFAGCPIVASDVGEVGAALSAGRVGVLVEPADADALASAIDGLL
                     GDPVRARALGERAARQAIAQYDLSRMVRRYANVYERAIGTRPRGLARLSARQEPSL"
     gene            2256..3188
                     /locus_tag="AC16CY_00685"
     CDS             2256..3188
                     /locus_tag="AC16CY_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352317.1"
                     /translation="MTMQLRALCGLAIVAFLIGAVRFFPSAYHAVGPACVGGDASNPN
                     VVMCDDFEDGAFERRWDIGGHQGRWPIDQFVLCTDGAFGFKDRCAAWSNHLVFDREWG
                     FYGYDARRPFAPQPEFYVRWYQYISNPYTWGPLEDKSVMLHDRSNTLVAYVGTNRNHL
                     PVEKDSGPGMPFVANYQDLDYPETGNRYNRVNRFQNQGRNITLEPGKWYLFEWYVKHN
                     TPGKSDGVTKLWVDDAGSPISRQTLRMEYNDMRWLRTMDAGKAFGELRLTVYDQRCDG
                     AHNTCPPRGPSILSQSHRWDHVAVSRAPIGPIAK"
     gene            3190..4014
                     /locus_tag="AC16CY_00690"
     CDS             3190..4014
                     /locus_tag="AC16CY_00690"
                     /inference="COORDINATES: protein motif:HMM:NF045027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase"
                     /protein_id="HIR3352318.1"
                     /translation="MNRAAGIVSFVALIAAAPVACSSPATGPGGLPPGGGAVLFSDDF
                     ESASLAAWEDGFDAARHRIQTDSSAGNRYLATTYPAGRDGGWLTRFIRPGGDSLYVSL
                     DVRFPNDWIGGTKLVGFYGSRNDDQWSAFGKAGLCPTGSDFFAAMVVTEAGGDPGPAR
                     FYTYYPAMSREPDGRTCWGRYGDGSESYTPPLTLSRGVWHRLEFFVQLNTPGRADGRQ
                     MFWIDGAQRGSWSGISFRDGVVLELNAVQLSFSVSGGVPRTQQLHVDNIVVRSAPA"
     gene            complement(4022..4954)
                     /locus_tag="AC16CY_00695"
     CDS             complement(4022..4954)
                     /locus_tag="AC16CY_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352319.1"
                     /translation="MKFHHAVVAKTYISWAVVCLVALGAPSLGTLAQSTGVIFTSSWD
                     TSQGALASAVTDGGRWPNYWEFNNGTGVKLLSVVPGGPGGHNALRVQQRGSSFAANVQ
                     IDNFLPQSQDYYLRYYMRNDDTSTEGDHVATVDTFQYANLTYMRKYSGLLTWNFHISL
                     YGCGYTYPIGHWGPALPLVNGQWYRFEYYVHYVDASHVQVYPRVYDANGLLIASDNTF
                     VQSGYLQALWNGRNDWTLASYYAAGHTFCVDPSYTNDFGLGNNGQYGADDTGKYWYFS
                     AVEIRTDRWPGPVGSSPPSAPPGPPTGVRVVGVN"
     gene            complement(5076..>5273)
                     /locus_tag="AC16CY_00700"
     CDS             complement(5076..>5273)
                     /locus_tag="AC16CY_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352320.1"
                     /translation="SASWNGRRDWNLASYYAAGQSFCVDPGWVNDFGLGNNGQRGAAD
                     TGQDWFFSAVEIRTDRWPGPA"
BASE COUNT          845 a         1685 c         1789 g          954 t
ORIGIN      
        1 cgctgacggc gtcgctcctc ggcttcgtcg tggccgcgtt ctttctgtcg cttgcgtatt
       61 ccgagatgtt gtacacgctg gtcgcgctgg cgatcgcctt cggcaaggtg accgacgcgc
      121 gggccccggt ggtcatgaaa ggcccggttc gtcgatgaga tctcgcccac ccgcttcctc
      181 cctcggcgcc ttcgcgtact acggcggtct ccgcctgctg ggtctcacgt cgctccggcg
      241 gcgtcaacaa gacggcggcc tgattctctg ctaccacaac gtcgtaccgg gcgacggcgg
      301 ccgggagggc gaccccggcc tgcacatgtc ctgcgtgcgt ttcgagcgac acatgcgctg
      361 gctcgccctt cactacaacg tggttccact gcacgagttc gtccagcgcg tggagatggg
      421 tgcgtcgctg caatccgttg cggcaatcac cttcgacgac gcctacgcgg gagtgttcgc
      481 gcatgctgcg ccgctgctcg aggcgctcga gctgcccgcc actgtgttcg tcgtggccga
      541 cgccccgggc cgatccgatc tcttctggtg ggatcggccc gacgtcgtga cattcgcggc
      601 gcggccggac acgcgtcaag agtggttgac gcagcttcag ggggaccagg cggcgattgt
      661 gtcgtcgctc ggggcgtccg ctgtcgcgcc gctgccgtcc acgcactacg ccgccgattg
      721 ggcgaccatc cgcgcacacg cgggccgcca actcgacatc ggcgtgcact cggcgacgca
      781 tcgatcgctc acgacgctgt ccgacgcaga cctcgaacgc gagatcgtca cgagccgcgc
      841 ggtgatctat cacgcgacgg gagtgtggcc acagtgcttt tcatatccct acggcctgtg
      901 ggatggccgg gtccgcgatg ccgtccgggc ggcaggctat cgcgccgcgt gtacgaccga
      961 tcccgagttc tacagcagac cggccgacgt ctgtgcgttg cccaggttga acgtcccggc
     1021 cggcatctcg gacggggcgt tcgaagcctg gacggccggt tttcggggga gcaaggcgtc
     1081 atgatgaatc gtcacccgcg agttgccggt cttcgtctgg cgcacctgat cgagtcgagc
     1141 gggcctggcg gcgccgagcg cgtggtcgca gagcttgcaa cctcgttgca ggcgggcggt
     1201 gcggacaacg tcgtattcct tcccgcggat ggggaagact ggcttcgacg ccagctcgaa
     1261 ggatctggag tggctgtcga atacttccgc atcgaacggc ccttctcggt ggcgtgcgcg
     1321 cggagcctgg cgctcggttt ccaaaagcat cgcatcgacc tcgcccacag ccatgagttc
     1381 tcgatggcgt tctatggggc ctgggcggcg cgactggctg gtgtgccgca cgtcatcacc
     1441 atgcacgggg gacggtacta tgcgggccgg ctccggcggc ggctggcgct ccgcgccgcg
     1501 atggcgctca gcgcccggac ggtggcggtc tccggtcgcc tggcgctggc catcggtcgc
     1561 gacctcggaa tccgagcgac gcgcatcctc acgatcccga acggcgttcg ctacctgccg
     1621 cccgaacggg tcactctgcg cgacgatctg cggctcggcc ccgacgatcg gctgatcgtt
     1681 gcggttggca atctgtatcc ggtcaaggga caccagtacc tgatcgacgc cgcggcgtcg
     1741 atcatcgacc ggcatccaac gcttcacatc gcgatcgccg gacgcggcga tcgcgaggcc
     1801 gcgctccgct cccaggccag agatcgcggc ctcgccgacc gcgtccacct gctgggcctt
     1861 cgcgcggaca tcccggcgat tctcgcggcc gctgatgcct tcgttctccc gtccctgtcc
     1921 gaggggctgc cgctggcgct catcgaagcg atgtttgccg gctgcccgat cgtcgccagt
     1981 gacgtgggag aggttggtgc ggcgctgtcg gcgggaaggg tcggcgtgct cgttgagccc
     2041 gcggatgccg acgcgctggc gagcgcgatc gatggcctcc tcggcgatcc cgtgcgcgcc
     2101 agggccctgg gagagcgagc cgcacgccag gccatcgcgc aatacgacct gtcccgcatg
     2161 gtccgccggt acgccaacgt ctacgagcgg gccattggaa cgcggcctcg tgggctggcg
     2221 cgcttgtcgg cccggcagga gccctctctc tgacaatgac catgcaactc cgagcgctct
     2281 gtgggttggc aatcgtcgcg ttcctcatcg gggcggtccg tttcttccca tcggcgtacc
     2341 acgccgtggg gcccgcgtgt gttggcggcg acgcttcgaa cccgaacgtc gtgatgtgcg
     2401 acgacttcga ggacggcgcg ttcgaacgcc gctgggacat cggaggccat caaggtaggt
     2461 ggccgatcga tcagttcgtc ctgtgcacgg acggcgcctt cggcttcaag gataggtgcg
     2521 cggcgtggag caaccacctg gtgttcgacc gcgagtgggg cttctacggg tacgatgcgc
     2581 gccggccatt tgcgccgcaa ccggaattct acgtgcgctg gtaccagtac atctcgaacc
     2641 cgtacacctg gggcccgctc gaggacaagt ccgtgatgct gcacgatcgg tcgaatacgc
     2701 tcgtcgccta cgtcggaacc aacagaaatc atctcccggt cgaaaaagac agcgggcccg
     2761 ggatgccttt cgtcgccaac tatcaggacc tcgactaccc ggagactgga aaccgataca
     2821 accgcgtgaa ccgttttcag aaccagggac ggaacatcac gctcgagcca gggaaatggt
     2881 acctcttcga gtggtacgtc aagcacaaca cacccgggaa gagcgacggc gtgacgaagc
     2941 tctgggtcga cgatgccggt tcgccaatct cacggcagac gctgcggatg gaatacaacg
     3001 acatgcggtg gctgcggacg atggacgccg ggaaggcgtt tggcgagctg cgcctgacgg
     3061 tgtacgacca gcgatgcgat ggagcgcaca atacgtgtcc gccgcgaggg ccgtccattc
     3121 tgtcgcagtc gcatcgctgg gatcatgtgg cggtgagcag agcgccgatc gggccgatcg
     3181 cgaagtgacg tgaatcgagc cgctggaatc gtgtcgttcg tcgcgctcat cgccgctgcg
     3241 cccgtcgcgt gcagttcgcc cgcaaccggg ccaggaggac ttccgccggg tggcggcgcc
     3301 gtcttgttca gcgacgactt cgagtcggcc tcgctcgccg cgtgggagga tggtttcgac
     3361 gcggcacgcc accgaattca gaccgattcg tcggcgggga atcggtatct cgccacgacc
     3421 tatcccgccg ggcgcgacgg cggatggctg acgcgtttca tcagaccggg cggcgactcg
     3481 ctgtacgtga gcctggacgt gcgcttcccg aacgactgga tcggcggaac gaaactggtg
     3541 gggttctacg gctcgcgaaa cgacgatcag tggtcagcgt tcggcaaagc cggactgtgc
     3601 ccgacgggca gcgatttctt cgctgccatg gtggtgacgg aagcaggcgg agatcctgga
     3661 ccggcgaggt tctacacgta ctatcccgcc atgtcgcggg agcccgacgg gcggacctgc
     3721 tggggacggt atggtgatgg ctcggagagc tatacgcctc cgctgacatt gagccggggc
     3781 gtgtggcacc gcctggagtt cttcgtgcag ctcaacacgc cgggtcgcgc cgacggacga
     3841 cagatgttct ggatcgacgg cgcacagcgg ggaagctggt cgggcatcag cttccgcgac
     3901 ggcgtcgttc tcgagctgaa tgccgtgcaa ctctccttca gcgtgagtgg gggagtgccc
     3961 cgtacccagc agctccacgt ggacaacatc gtcgtgcgca gtgcgcccgc gtgacatcgg
     4021 ttcagttgac gccgacgact ctgacgccgg ttggcgggcc gggcggtgcg cttggcgggc
     4081 tcgagccgac ggggccaggc catcgatcgg tacgaatctc gacggcggag aagtaccagt
     4141 acttcccggt gtcgtcggct ccgtactggc cgttgttccc cagcccgaaa tcgttcgtgt
     4201 agctgggatc gacgcagaag gtgtgccccg ccgcatagta ggacgcaagc gtccagtcgt
     4261 ttcgtccgtt ccagagggcc tgcaggtagc ccgactgaac gaacgtgttg tctgacgcaa
     4321 tcaggagtcc gttggcgtcg tacacacgcg ggtacacctg aacgtggctg gcatcgacgt
     4381 agtgaacgta gtactcgaag cggtaccact gtccattgac caggggcagc gcggggcccc
     4441 agtgcccgat cgggtacgtg tagccacagc cgtacaagct gatgtggaaa ttccaggtca
     4501 gcaggcctga gtactttcgc atgtaggtca ggttcgcgta ctggaacgtg tccacggtgg
     4561 cgacgtgatc gccctcggtc gaggtgtcgt cgttcctcat gtagtaccgc aggtaataat
     4621 cctgggattg cggcaggaag ttgtcgatct ggacgttggc cgcgaagctg gagcctcgct
     4681 gctgaacgcg cagcgcgttg tgcccgccgg gtcctccagg gacgaccgac aggagcttga
     4741 cgccagtgcc gttgttgaac tcccagtaat tgggccaccg gccgccgtcg gtgacggcgc
     4801 tggccagagc tccttgcgac gtgtcccagc tgctcgtgaa gatcaccccg gttgactggg
     4861 ccagcgttcc caaggaaggc gcgccgagcg cgacaagaca gacgactgcc cagctgatgt
     4921 aggttttcgc gacaacagcg tggtggaatt tcacggagcc gctgaatcgg ggcgggccat
     4981 tgagcgagga ttgtgcaaca agcgctccag tcgaggttcg agccggcgtg gtcgcgccgc
     5041 cggacgcctt caaagagagt gccgccccca ggtcatcacg cgggacccgg ccatcgatcg
     5101 gtgcggattt cgaccgctga gaaaaaccag tcctgacccg tgtcggcggc tccgcgttgg
     5161 ccgttgttgc cgagcccgaa gtcgttcacc cagccgggat cgacgcagaa gctctggccg
     5221 gcggcgtagt aggacgccag gttccagtcg cgccggccat tccaggatgc aga
//
LOCUS       DBNGSH010000035         5043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_511968, whole genome shotgun sequence.
ACCESSION   DBNGSH010000035 DBNGSH010000000
VERSION     DBNGSH010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_511968"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="AC16CY_00705"
     CDS             <1..504
                     /locus_tag="AC16CY_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3352321.1"
                     /translation="RWVTSLGDLTWINMIGQTPFIRASMSGDITTMRLLLERGADPKI
                     TTVGGTTALVTAAGGNVAVQQSFVESRESSMQAVKLCLELGIDVNAANETGFTAVMGA
                     ANKGWDDILEVLLQAGGRLDVADAQERTPLRWAKGEFIALHPPEEKSSTVALIEKYTK
                     ERAGRSR"
     gene            501..2813
                     /locus_tag="AC16CY_00710"
     CDS             501..2813
                     /locus_tag="AC16CY_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3352322.1"
                     /translation="MTRKAVLLSASVLVVAAVLGGHAQVRPDTTSVRDQALVDKYCVT
                     CHSERTKSGNLTLAGLDATRPGDHPETFEKVVRKVRAGLMPPAGLPRPDRAALDGFAA
                     SLEAGLDRAAASRPNPGSPGLHRMNRTEYGNAIRDLLDLDVDVATLLPADDSSEGFDN
                     IADALRVSPALVERYTSAAVKISRLAVGNMLLTASTVTYRVPGDLSQSGHLDGQPLGT
                     RGGTLVEHTFPLDAEYAFKIRVRGAAIGLAAANLGGEEIEFSVDGERIRLVPATATID
                     LKLPMKAGPHTLGVTFVRKAPAGADEVWGVYATNSGVQSVAITGPLAPSGLGDTPSRR
                     RIFVCRPVEGEDAGCARRILRTIARRAFRQPLTDTDLQMLNDFYESGRQNGGFDAGIE
                     QGLARILVDPRFVFRLEREPAGAVPGRPYRVSDLELASRLSFFLWSSIPDEELLDAAI
                     AGTLHEPAVLERQTRRMLADPKADALVSSFGGQWLFLRELKNARPETSGFSENLRQAF
                     RRETEMLLASIMREDRSVIDLLDADYTFVDETLARHYGIPNVRGSRFRRVPVASDARR
                     GLIGHGSFLLVTSVANRTSPVSRGKWVLENLMGVPPPLPPPNVPSIDESPEAAKASSL
                     RQKLEVHRGNPACSACHRIMDPIGFTLENFDLVGRWRTTDGGVPIDATSQLFDGTSLD
                     GPASLRRALLDRSTVFVRTMTEKLMVYGTGRALKHDDMPVVRKIARDAAQDGHRFSAL
                     ILGIVRSDPFQMRMMEQGAGGKGVVFRPEK"
     gene            2848..4173
                     /locus_tag="AC16CY_00715"
     CDS             2848..4173
                     /locus_tag="AC16CY_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3352323.1"
                     /translation="MAFITKKHVPRRTVLRGLGATIALPLLDSMVAAQTPIRKTAAAA
                     RSRLSCIYVPHGATMDKWTPSATGKGFEFTEILTPLEPFRDRVTVVSNLAHAKAGAVG
                     SDAGADHARSAAVFLSGAQPARDSIQVGETIDQIAVRHIGQDTPLPSLELSIEDVALS
                     CGSGYACAYSNTISWKNATTPLPMENNPQVVFEKLFGDGGNDADRAARRRQSRSLLDS
                     VLGQVASLSHDLPAGDRVRLSEYLENVREIERRIQKADTQFPSDLALPDAPVGVPESF
                     DEHFKIMYDLQVLAFRAEITRIATMMYARDTSLSVYPQSGVRESFHVASHHSNARAQM
                     DRFAVINRYHVQMLAYFLDKLKKTPDGDGTLLDHSLVLYGSSMSNGNQHDHDPLPVIL
                     AGGASGRLAGGSHVQSAPHTPMSNLLLAMLDKLGIEQSQHGDSTGALDL"
     gene            4266..>5043
                     /locus_tag="AC16CY_00720"
     CDS             4266..>5043
                     /locus_tag="AC16CY_00720"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3352324.1"
                     /translation="MTVTAGTRLGPYEIVGPLGAGGMGEVYRARDTRLQRDVAIKVLP
                     PHVSAAPELRERFEREARAVASLNHPNICVLHDVGRHEETDFLVLEFLDGETLASRLA
                     RGQLPLDHALAIATQLADALAAAHRAGVVHRDVKPGNVLLLRKAASGDSRWAGAPVAK
                     LLDFGLAKAAGSAVGPAKAGHYDGPAGDPGDRSVRLQPDLTSLPTTPQNLTVQGTILG
                     TVEYMAPEQIEGQEADARTDIFALGVVVYEMLTGRRAFSGR"
BASE COUNT          833 a         1731 c         1711 g          768 t
ORIGIN      
        1 cggtgggtca cctcgctcgg cgacctgacc tggatcaaca tgatcgggca gacgccgttc
       61 atccgggcct cgatgtcggg cgacatcacg acgatgcgcc tcctgctgga gaggggggcg
      121 gacccgaaga tcaccacggt cggcggaacg acggcgctcg tgactgccgc gggcggcaac
      181 gtcgccgtgc agcagtcatt cgtcgagtcg agggaatcgt ccatgcaggc cgtcaagctg
      241 tgcctcgagc tcggcatcga cgtgaacgcc gcgaacgaaa ccggcttcac tgcggtcatg
      301 ggcgcggcca acaagggatg ggacgacatt ctcgaggtgc tgctccaggc gggcggccgc
      361 ctcgacgtcg cggacgcgca ggagcggaca ccgcttcgct gggcgaaggg cgagttcatc
      421 gcgctgcacc cgccggaaga gaaatcctcg acggttgcgc tcatcgagaa gtacacgaag
      481 gaacgggctg gccgtagccg atgacgagaa aagccgtcct gctgtcggcc tccgtcctgg
      541 tcgtcgccgc cgtcctcggt ggtcacgcgc aggtcaggcc cgatacgacg tcggtgcgcg
      601 atcaggcgct cgtggacaag tactgcgtga cctgtcatag cgagcgaacg aagtcgggca
      661 acctgactct ggccgggctc gatgccacca ggcccggcga ccatccggag acgttcgaga
      721 aagtcgtcag gaaagtgcga gccgggctga tgcctccggc gggcctgccg cgcccggacc
      781 gtgcggccct cgacggtttt gcggccagcc tcgaagccgg gctcgatcgc gctgccgcgt
      841 cgaggcccaa ccccggcagc cccgggctgc accggatgaa ccgcaccgag tacggcaacg
      901 ccatccgcga tctcctggat ctcgacgtgg acgtcgcgac gctgctgccc gcggacgatt
      961 cgagcgaggg gttcgacaac atcgcggatg ccctgcgcgt gtcgcccgcg ctcgtcgagc
     1021 ggtacacgtc cgcggccgtc aagatcagcc ggctcgccgt cggcaacatg ctgctcacgg
     1081 cgtcgacggt cacctaccgc gtgcccggtg atctgtcgca atcaggtcac ctcgatggtc
     1141 agccgctcgg cacgcgcggg ggcacgctcg tcgaacacac gtttccgctg gacgccgagt
     1201 acgccttcaa gatccgcgtc agaggcgcgg ctatcggcct ggccgcggcg aacctgggcg
     1261 gcgaggagat cgagttctcc gtggacggcg agcggatccg cctggtgccg gcgacagcca
     1321 cgatcgatct caagctgccg atgaaagccg gtccccacac gctgggcgtc acgtttgtcc
     1381 ggaaggcgcc ggcgggcgcg gacgaggtct ggggtgtgta cgcgaccaac tcgggcgtcc
     1441 agagcgtggc cattaccggg ccgctcgcgc catcgggact gggcgacacg ccaagccggc
     1501 gccgcatctt cgtgtgccgt cccgtcgaag gcgaggacgc cgggtgcgcg cggcgcattc
     1561 tcaggacgat cgcgcggcgc gcgttccgcc agccgttgac cgacaccgac cttcagatgc
     1621 tgaacgactt ctacgagtcg ggccggcaga acggcggctt cgacgccggc atcgagcagg
     1681 ggctcgcgcg aatcctcgtg gatccccgct tcgtcttccg cctcgagcgg gagcctgccg
     1741 gcgccgttcc cggccggccg tatcgcgtct cggatctgga gctggcctcc cgtctctcct
     1801 ttttcctctg gagcagcatt ccagacgagg agttgctcga cgcggcaatc gcgggaacgc
     1861 tgcacgagcc ggcggtcctg gaacgccaga cgcgccgcat gctggccgat cccaaggcgg
     1921 acgcgctggt cagcagcttc ggcggccagt ggctcttcct gcgcgagctg aagaacgccc
     1981 gccccgagac gtcgggcttc tccgagaacc tgcggcaggc cttccgtcgc gagaccgaga
     2041 tgctgctcgc gagcatcatg cgtgaagacc ggagcgtgat cgacctgctc gacgcggact
     2101 acacgttcgt ggacgagacg ctcgcgcgcc actacggcat cccgaacgtg cgcggcagcc
     2161 ggttccgccg agtgccggtc gcgagcgatg cgcgccgcgg cctgatcggg cacgggagct
     2221 ttctgctggt cacctcggtg gccaaccgca cctcgcccgt gtcgcgcggc aagtgggtgc
     2281 tcgagaacct gatgggagtc ccgccaccgc tgccgccgcc caacgtgcct tccatcgacg
     2341 aaagcccgga ggccgccaag gcctcgtcgc tccggcagaa gctcgaggtt catcgcggca
     2401 acccggcctg ttcggcgtgc caccgcatca tggacccgat cgggttcacg ctcgagaact
     2461 tcgacctggt cggacgatgg cgcacgaccg acggcggcgt gccgatcgac gcgacgagtc
     2521 agctcttcga cggcacgtcg ctcgacggcc cggccagcct gcggcgggcg ctgctggatc
     2581 gctcgaccgt gttcgtgcgc acgatgacgg agaagctgat ggtgtacggc accggccgcg
     2641 cgctgaagca cgacgacatg ccggtggtca ggaagatcgc tcgcgacgcc gcgcaggacg
     2701 gccatcggtt ctctgcgctc atcctcggga ttgtccgcag cgacccgttc cagatgagga
     2761 tgatggagca gggggcaggg ggcaaggggg tagtctttag acccgaaaag tagaggcggg
     2821 tctttagacc cgcctgggag atcccgaatg gctttcatca ccaagaaaca cgtgccgcgc
     2881 cgcaccgtcc ttcgcggcct tggcgccaca atcgctctgc cgctgctgga ctcgatggtc
     2941 gcggcgcaga cgccgatccg gaagaccgcc gctgccgcca ggagccgctt gagctgcatc
     3001 tacgtcccgc acggcgcgac gatggacaag tggacgccgt cggcgacggg caaggggttc
     3061 gagttcaccg agatcctgac gccgctcgag ccgttccggg accgtgtcac ggtcgtgtcc
     3121 aacctggcgc atgccaaggc gggcgccgtg ggctccgacg ccggcgccga tcacgcccga
     3181 tcggcggccg tcttcctcag tggcgcgcag ccggcccggg actcgatcca ggtgggggag
     3241 acgatcgacc agatcgcggt gcgccacatc gggcaggaca ccccgctccc gtcgctggaa
     3301 ctgtcgatcg aggacgtcgc gctgagctgc ggctccgggt acgcgtgcgc ctactcgaac
     3361 acgatctcct ggaagaacgc gaccacgccg ctcccgatgg agaacaaccc gcaggtggtg
     3421 ttcgagaaac tgttcggcga tggaggcaac gacgccgacc gcgccgcgcg gcgccggcag
     3481 agccgcagcc tgctcgactc ggtcctcggc caggtggcct cgctcagcca cgatctgccc
     3541 gcgggggatc gcgtccgcct ctcggagtac ctggagaacg ttcgcgagat cgagcggcgc
     3601 atccagaagg ccgacacgca gttcccgagc gacctggcgc tccccgacgc gccggtcggt
     3661 gtgcctgagt ccttcgacga gcacttcaag atcatgtacg acctgcaggt gctcgccttc
     3721 cgggccgaga tcacgcgcat cgccacgatg atgtacgcgc gcgacacgag cctgtcggtc
     3781 taccctcaga gcggcgtgcg cgagagcttc cacgtcgcgt cgcaccactc gaacgcgcgc
     3841 gcgcagatgg accggttcgc cgtcatcaac cggtaccacg tgcagatgct cgcctatttc
     3901 ctggacaagc tgaagaagac gccggacggc gacgggacgc tgctcgatca ctcgctggtg
     3961 ctctacggca gcagcatgag caatggcaac cagcacgacc acgatccgct tcccgtgatc
     4021 ctggcgggcg gcgcgtcggg ccggctcgcg ggcggcagcc acgtgcagtc cgcgccgcac
     4081 acgccgatgt cgaacctgct gctggccatg ctcgacaagc tggggatcga gcagtcgcag
     4141 cacggcgaca gcaccggggc gctcgacctg tgaggcattg cggtgaaatc accattcagc
     4201 gatcgataat caacatcgat tcaccaatca cagatcgcga aatcatcaat gcgttgtagc
     4261 cggtcgtgac agttacagcg ggcacacgcc tgggccctta tgagatcgtc ggtccgctcg
     4321 gcgcgggcgg catgggcgag gtatatcgcg cgcgcgatac gcggctgcag cgcgacgtcg
     4381 cgatcaaggt gctgcccccg cacgtgtccg cggcgccgga gcttcgcgag cgattcgagc
     4441 gcgaggcgcg cgccgttgcg tccctgaacc atccgaacat ctgcgtgctc catgacgtcg
     4501 gtcgccacga ggagaccgac ttcctcgtcc tcgaattcct cgacggcgag acgctcgcca
     4561 gccggctggc cagaggccag ctcccgctcg atcatgccct cgccatcgcc acgcagctcg
     4621 ctgacgccct cgcggccgcg catcgagcgg gcgtcgtgca ccgcgacgtg aagcccggca
     4681 acgtgctcct gcttcgcaaa gctgccagcg gcgacagtcg atgggccggc gcgccggtgg
     4741 ccaagctcct cgacttcggc ctcgcgaagg cggctggttc tgccgttggt ccggctaaag
     4801 ccggacacta cgacggtccg gctggcgatc ccggcgatcg tagtgtccgg cttcagccgg
     4861 acctcacgtc cctgccgacg acgccgcaga acctcaccgt gcaggggacg atcctcggca
     4921 cggtggagta catggccccc gagcagatcg agggacagga ggccgacgcg cgaaccgata
     4981 tcttcgcgct cggggtcgtc gtctacgaga tgctgacggg gaggcgcgcg ttcagcggcc
     5041 gga
//
LOCUS       DBNGSH010000036         3539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3907808, whole genome shotgun sequence.
ACCESSION   DBNGSH010000036 DBNGSH010000000
VERSION     DBNGSH010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3907808"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="AC16CY_00725"
     CDS             complement(<1..787)
                     /locus_tag="AC16CY_00725"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3352325.1"
                     /translation="MTPFTTLRRIGAPVASAVATALVFIVVLWVQHARSAWPFDQPVG
                     TPRVESMAGMESTSGANTHNRVPVDAAAARSLDIRLETVGRQSLTQTVRAVATIVPDE
                     SRISHVHTRVAGWIEHLDVNTTGEEVRAGQPLAHIFSQELLSSQTEYLSARRNLSASG
                     ISSAVIASGRTRLGVLGMTAAEIDEIERNGEPKRLVTVVAPSSGVVVNRGVTVGTSVD
                     PSTTLLTIADLSHVWVLAEVPEASIPSVRVGSKALLDFPASGRS"
     gene            complement(784..2040)
                     /locus_tag="AC16CY_00730"
     CDS             complement(784..2040)
                     /locus_tag="AC16CY_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3352326.1"
                     /translation="MKGFSFSIVQLVVTLIASPVAATAQDSKPLPSPLSLADVIRIAG
                     ERRDEIHAARARTRAGEVRPVIVSALPDPMLSPSLDHLPFMFNGADFSVVIEQQIPLS
                     GIRSHRRASSLADLDRLRAEANRTTLDVGIEAANTYLMLQERRRTAALLDEQVAFARD
                     VVSAANARYASGTAPQSDVLRAEVEVARLEATARALVAEVRGAEAMLNTSLALDADTP
                     VPPLTNLTLSQPVPTWSAIRMALRSRPELAAGRAEIARAEAEVLVMRDMFRPMATIRT
                     GPSYTMAEGRGWMAMVGLSLPIWRSKLKAGVAEAEAMRAMSEADLRAMTRMIEGDAAA
                     AISQLQAARDRQMALTADVLPRARMAIAPAVASYGAGQLPLVSVIEAIQSLWSVQSDL
                     ITADTQLGLAWARLGRAIGSYEAIVQ"
     gene            complement(2100..2789)
                     /locus_tag="AC16CY_00735"
     CDS             complement(2100..2789)
                     /locus_tag="AC16CY_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352327.1"
                     /translation="MRTDSIRVLASAAIAFAAFTNTANAQTGSPILNAFDVQKLVAST
                     EPADNARLSAHFAALADHYTREATRHTAMAQAFIAAPTRRTPANTAADHCKRLAALNK
                     QSADTLRELAAYHDKRAAGVVAAPPKGAARFQAGTGAPEPADDQLSALAARASTPADH
                     HALEEYFQTTAKRYREAVNEHSSMAQAYRGTRIAGAAVHCDRLVSLSRDEAKEATAAA
                     EMHKQLATAAR"
BASE COUNT          592 a         1172 c         1155 g          620 t
ORIGIN      
        1 gcgagcggcc agacgctgga aagtccagca gcgccttgct gccgacacgg acggacggaa
       61 tgctcgcctc ggggacctcg gccagcaccc acacgtgcga caggtccgca atcgtgagca
      121 gcgtggttga gggatccacc gacgtgccga cggtaacccc acgattcacc acaaccccac
      181 tgctcggcgc gacgactgtc acgaggcgct tgggttcacc attccgctcg atctcatcga
      241 tctccgcggc ggtcattccc aggacgccca gccgcgtgcg tccgctcgcg atcacggcgc
      301 tgctgatgcc cgaggccgat aggtttcttc tcgcagacag atattcggtc tgcgacgaca
      361 gcaactcctg cgaaaagatg tgcgcgagcg gctggcccgc gcgaacttcc tcacccgtcg
      421 tgttgacgtc gagatgctcg atccagcccg ccacgcgggt atgcacgtgt gaaatccgtg
      481 actcgtcggg cacgatcgtg gccacggccc gcacggtctg cgtcagcgac tgtcgcccga
      541 ccgtctcgag ccgaatgtcc agcgaccgcg cagcggcggc atcgaccgga acccggttgt
      601 gcgtattcgc cccgctcgtt gactccatgc cggccatgct ttcgacacgt ggtgtgccaa
      661 ccggctgatc aaagggccat gcggagcggg cgtgctgtac ccacaacaca acgatgaaga
      721 cgagcgcggt tgcgaccgcg gaggcgacgg gagcgccgat gcgtcggagc gtagtaaagg
      781 gtgtcattgg acaatcgcct cgtacgatcc aatggcgcga cccagtcgcg cccaggcaag
      841 accgagttgt gtatctgcgg taatgagatc ggactggacg gaccagagcg attgaatagc
      901 ttcgatgacg ctgaccaagg ggagctggcc cgccccgtag ctggccacag ccggcgcgat
      961 cgccatccga gcgcggggca agacgtcggc cgtcagcgcc atctggcgat cccgcgccgc
     1021 ctgcagctga ctgatggccg ctgccgcgtc accttcgatc attcgcgtca tcgcgcggag
     1081 gtccgcttcc gacatcgcgc gcatcgcttc cgcttcggcc acacccgcct tcagcttgct
     1141 gcgccagatc ggcaggctga ggcccaccat cgccatccaa ccgcggcctt cggccatcgt
     1201 gtaggacggt ccggtgcgaa tcgtcgccat cggccggaac atgtcgcgca tcaccagcac
     1261 ttcggcttcg gcgcgcgcga tctcggctcg tcccgcggcc aactccgggc gtgacctcaa
     1321 tgccatcctg atcgccgacc acgtcggcac cggctgcgac aacgtcaggt tggtcaatgg
     1381 gggtaccggc gtatcggcgt ccagcgcgag gcttgtgttg agcatcgcct cggcgccacg
     1441 cacttcggcg accagggcgc gagcggtcgc ttcgagtcgc gccacttcca cttcagcgcg
     1501 gagcacgtcg gattgcggcg cagtgccgct ggcgtatcgc gcattcgcgg cgctgacgac
     1561 atcacgtgca aaggcaacct gctcgtcgag gagtgcggca gttcgacgac gttcctggag
     1621 catcaagtac gtattcgccg cctcgatccc cacgtcgagg gtcgtgcgat tcgcctcggc
     1681 acgcaggcga tccagatcgg ccagtgagga tgcgcggcga tggctgcgaa tgcctgacaa
     1741 cggaatctgc tgctcgatga cgacgctgaa gtccgccccg ttgaacatga acggcaagtg
     1801 gtcgagcgat ggcgagagca tcggatccgg cagtgcggat acgatcaccg ggcgcacttc
     1861 gccggctctc gtgcgcgcac gtgccgcgtg tatctcgtct cttcgctcgc cggcgatgcg
     1921 gatgacatcg gcgaggctca gtggcgaggg cagtggcttc gaatcctgcg cagttgcagc
     1981 tacaggtgac gcgatcagcg tcaccaccag ttgcacgatc gagaaagaga agcccttcat
     2041 gtcagtcacc gagtcgaatg gctcggcacg ggccggctgt taccggcccg tgtcccgctt
     2101 taccgagcgg cggttgcgag ctgcttgtgc atctctgcag ctgccgttgc ttccttcgct
     2161 tcgtcccgcg acaggctcac gaggcggtcg cagtggaccg ccgccccggc gatccgcgtg
     2221 ccccgatagg cctgcgccat cgaagagtgt tcgttcaccg cttcgcggta gcgtttcgcc
     2281 gtcgtctgga agtactcctc cagtgcgtgg tgatccgccg gcgtgctggc acgtgcggcg
     2341 agtgcgctga gctgatcgtc cgctggctcg ggcgcaccgg tccccgcctg gaatcgagcg
     2401 gccccttttg gtggcgcggc cacgacgcct gcagcccgct tgtcgtgata ggccgccagc
     2461 tcgcggagcg tatccgccga ctgtttgttg agcgccgcca gacgcttgca gtggtcggcg
     2521 gcggtgttgg cgggcgtccg tcgcgtgggc gccgcaatga atgcctgcgc catggcggtg
     2581 tgacgtgtgg cttcacgcgt gtagtgatcg gccagagcgg cgaaatgagc actcagacgg
     2641 gcgttgtccg ccggctccgt gctcgccacc agcttctgaa cgtcgaaggc gttcagaatc
     2701 ggcgagccgg tttgggcgtt cgcggtgttc gtaaaggcgg cgaacgcaat cgcggcgctc
     2761 gcgagcaccc gaatcgaatc cgttctcatg acagacctgc cgtttctgtg accttcgtca
     2821 cgctgcgagt tgcaggttcg gaccaagtcg gggagcgggc ctcgacgccc gcccttcgat
     2881 cgaggcctgt tgtacgggga ttgccgcgac tcagattcgg agggcctgaa cgagaggtcg
     2941 gatttcgagc cgccgacgcc cgacagcctc gtacgctgaa aggctgctgg aaacccgtgg
     3001 cgtgaatgcg aaccgcggaa ccaggactcc gctgtgaaca gtcggtgcca aggcgctgcg
     3061 gcgaacgtcc tcgcgcacga acacggtcgc gttgccggtg accgtgcagt cgtcgtaggc
     3121 gatgatgcct gacgacgctg cctccgcttg gtgctcgcac gcgccggcca tttctccacc
     3181 ggagtgacac caaacaccgc acaacagggc ggcggcgttc tgcccggcgg ccagcgtcaa
     3241 cacggtcgaa aggagggctg ctcgaaacac gtcagtgctc ccgactctcg agggcaagac
     3301 ccgcgccagg ccgcccggcg gcgccacccg tgaatcgcag cgtcttcttg acgtgtcaac
     3361 gatccgcctt cttgagcctg cggaatttcg ccaagacggt gccgaaaatt ccacaccgcg
     3421 tgttccggca agctaaacgg ggccaccggg ccggcgggca cggcctggca ccagagctgc
     3481 tgtgcagagc atctggagga gttatggcgt tacgccactc tgaaagcagc gccctcatc
//
LOCUS       DBNGSH010000037         3365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1700427, whole genome shotgun sequence.
ACCESSION   DBNGSH010000037 DBNGSH010000000
VERSION     DBNGSH010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1700427"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="AC16CY_00740"
     CDS             complement(<1..1061)
                     /locus_tag="AC16CY_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR3352328.1"
                     /translation="MARLFGTDGVRSKAGESPLDAPTIRRLGAALVRALRHHDAPGRR
                     HEADIRFLAGRDTRESGSWIERELAFGIQSQGGALTSAGIVPTPAIAYLTPRTGYSAG
                     VVISASHNPFEDNGIKVFSGAGEKFTETLERQVESIVDDPAWSVPGDPAGPVEEVDFR
                     GEYIDHLRAILPPGRTPAGMRVAIDCANGATATVAPRLFRDLGFDVSTIGVAPDGRNI
                     NLHCGSTAPEGLARLVVDGGHQLGVAFDGDGDRAIFVDARGRVVDGDAVMFMCAKQMK
                     AEGRLKGNAVVATVMSNIGLELALREAGIDIVRCPVGDKYVMEEMIRRDLSLGGEQSG
                     HVIFSEYLFTGDGLATALSV"
     gene            complement(1069..1728)
                     /locus_tag="AC16CY_00745"
     CDS             complement(1069..1728)
                     /locus_tag="AC16CY_00745"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR3352329.1"
                     /translation="MALVGLRHPGLKIVSVILAALLWLLVSGEQEVERALRVPLEFTN
                     LPKHLELVGEPPSLVDVRVRGSSGTISRVAAAELAAVLDVRDAKDGLRRFNLQTTDVR
                     APFGVEVIQVAPASVELTFEESGSKTVPIMARTAGDPAPGFVVSGIAVEPPNVTVSGP
                     VSALSGVKEATTDPVSVSNAKASVTETVTIGIANAAVRLGEIRSARVAVTITMDPGAK
                     P"
     gene            complement(1730..2548)
                     /gene="cdaA"
                     /locus_tag="AC16CY_00750"
     CDS             complement(1730..2548)
                     /gene="cdaA"
                     /locus_tag="AC16CY_00750"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR3352330.1"
                     /translation="MEWLSELLRRPEVSWWDLLDITLVAVVIYELLVLIRGTRAVQMA
                     LSAGFLIGLFFVSRWLDLETVNWVIRNLATYVGFAIIVLFQADIRRALAHFGRAPVFR
                     YFERAASADETLEDLVVAATTLAARRIGAIIVIERQIGLRNYIEGGIPLDATVTYDLL
                     ATIFQPGSPLHDGAAIVQGDRIAAAACFLPLSVNPRVSRELGTRHRAALGLTEENDAV
                     AIVVSEETGAVSLAVAGGLERGLTADQLRERLRTLFGVHRRERRAQAQARSTVA"
     gene            complement(2548..>3365)
                     /gene="folP"
                     /locus_tag="AC16CY_00755"
     CDS             complement(2548..>3365)
                     /gene="folP"
                     /locus_tag="AC16CY_00755"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3352331.1"
                     /translation="GGRTLVMGIINVTPDSFSDGGRLLDPARAVDAGVRMVEEGADVL
                     DIGGESTRPGARPVGADDERRRILPVIEGLAARVQVPLSVDTYKASIADLALRAGASI
                     VNDISGLRYEPELASVVASHGAAIVLMHTRGRSVDMYQEAVYTDVVDEVLDELRASIA
                     FAAGAGVRKQSVLVDPGLGFAKEASHTYEALARLAEFGDLGHPVVVGPSRKAFLTRPL
                     GKDVPAIERDWLSSAAVTAAVLAGAHVVRVHAVGQMSQVARVADEIRKYHREH"
BASE COUNT          556 a         1203 c         1113 g          493 t
ORIGIN      
        1 acgctcaacg ccgtagcgag tccgtccccg gtgaacaggt actcggagaa gatgacgtgg
       61 ccggactgct cgccgcccag ggagaggtcg cgccgaatca tctcttccat cacgtacttg
      121 tcgccgaccg gacagcggac gatgtcgatg cccgcctccc gcagcgccag ctcgaggccg
      181 atgttgctca tcaccgtggc gacgacggcg ttgcccttca accggccctc agccttcatc
      241 tgcttcgcgc acatgaacat gacggcatcg ccgtcaacga cacggccgcg ggcatcgacg
      301 aagatggcgc ggtcgccgtc gccgtcgaag gccaccccca gctgatggcc gccgtcgacg
      361 acgagacgtg cgagcccctc cggcgcggtc gacccgcagt ggaggttgat gttgcggcca
      421 tcaggcgcga ccccgatcgt gctcacgtcg aagcccaggt cgcgaaagag tcgcggcgcc
      481 accgtggcgg tggccccgtt ggcgcaatcg atcgccacgc gcatgcccgc cggcgttcgg
      541 ccgggaggca ggatcgccct caggtgatcg atgtactccc cgcgaaaatc gacctcttcg
      601 accgggcccg cgggatcgcc tggaaccgac cacgcggggt cgtccacaat cgactcgacc
      661 tgccgctcga gcgtttcggt aaacttctcc cccgcgccgg aaaagacttt gatgccgttg
      721 tcttcgaaag ggttgtgcga cgccgagatc acgacgcccg cggagtaacc cgtccgcggc
      781 gtcagatagg caatggcggg tgtgggcacg ataccggcgc tcgtgagcgc gccaccctgg
      841 ctttgaatgc caaaggcgag ctcgcgctcg atccaggatc ccgactcgcg cgtgtcgcgg
      901 ccagcgagga aacggatgtc ggcctcgtga cgccgaccag gggcatcgtg atgacgaagc
      961 gcgcggacca gcgcggctcc gaggcgccgt atcgtcggcg cgtcgagcgg agactccccc
     1021 gctttgctcc tgacgccatc ggttccgaac agtcgcgcca tcggtctgct agggcttcgc
     1081 gccaggatcc atcgtgatcg tcacggcgac tcgcgcgctc cggatctcgc ccaggcggac
     1141 ggccgcgttc gcgatcccga tcgtcaccgt ctcggtcaca gacgccttcg cgttgctcac
     1201 ggagaccggg tcggtggtcg cttccttcac accggacaac gcgctcaccg gtccgctcac
     1261 cgtcacgttg gggggctcga cggcgatgcc gctcacgacg aatccgggag ccgggtcgcc
     1321 ggccgttcgc gccatgatcg gcacggtttt cgagcccgac tcctcgaagg tcagctccac
     1381 gctcgcgggg gcgacctgga tcacctccac gccgaacggc gcgcgcacgt ccgttgtctg
     1441 caggttgaac cgccgcagcc cgtccttggc gtcgcgcaca tcgaggacgg cggcaagctc
     1501 ggccgccgcc acccggctga tcgtgccgga cgagcctctc acgcggacat ccaccaggga
     1561 cggcggctcg cccaccagct cgaggtgctt cggaaggttc gtgaactcga gcgggacgcg
     1621 cagcgcgcgt tcgacctcct gctcgcccga cacgagcagc cagagcagcg cagcgaggat
     1681 gaccgacacg atcttgagtc cgggatggcg cagtccgacg agcgccatct caggccaccg
     1741 tgctccgcgc ctgggcctgg gcgcggcgct cccgccggtg cacaccgaac agggttctga
     1801 gccgctcgcg gagctggtcg gcagtcagcc ctcgctccag gcccccggcg accgcgagcg
     1861 acaccgcccc ggtttcctcg gacacgacta tggcgaccgc gtcgttctcc tcggtcaggc
     1921 cgagcgccgc gcgatgccgc gtgccaagct cccggctgac ccgcggattg accgacaggg
     1981 gcaggaagca ggccgcggcc gcgatccggt ctccctgcac aatcgcggcg ccgtcgtgca
     2041 gcggtgaccc gggctggaag atcgtcgcga gcagatcgta ggtgaccgtg gcatcgagcg
     2101 ggatgccgcc ctcgatgtag ttccgcaggc cgatctgccg ctcgatcacg atgatcgcgc
     2161 cgatacgcct tgccgcgagc gtcgtcgccg cgaccacgag atcctcgagc gtctcgtccg
     2221 cgctcgctgc gcgctcgaag tacctgaaga cgggcgcgcg cccgaagtgc gccagcgccc
     2281 gccggatgtc cgcctggaac aacacgatga tggcgaagcc gacgtaggtc gcgaggttgc
     2341 ggatgaccca gttcaccgtc tcgagatcga gccagcgcga gacgaagaac aggccgatca
     2401 ggaacccggc gctgagcgcc atctgcaccg cgcgggtgcc gcgaatgagc accagcagct
     2461 cgtagatcac caccgcgacg agcgtgatgt cgaggaggtc ccaccaggag acctccgggc
     2521 ggcgcagcag ctctgacagc cactccatca gtgctcgcga tgatacttcc gaatttcgtc
     2581 ggccacgcgc gcgacctgcg acatctgacc gacggcgtgc acgcggacga cgtgcgcgcc
     2641 ggccaggacg gcagccgtga cggcggcgct cgacagccag tcgcgctcga tggcaggcac
     2701 gtccttcccg agcggccgcg tcagaaacgc cttcctggac ggcccgacga cgacgggatg
     2761 accgaggtcg ccgaactcgg cgagcctggc aagggcctca taggtatgcg acgcctcctt
     2821 ggcgaatccg aggccggggt ccaccagcac tgactgcttg cgaacaccgg cgcccgccgc
     2881 aaacgcaatg ctcgcgcgca gctcgtcgag gacctcgtcg accacgtcgg tgtagacggc
     2941 ctcctggtac atgtcgacag accggccgcg cgtatgcatg aggacaatgg ctgcaccgtg
     3001 gcttgccacg acggaggcca gttccggctc gtaccggagg ccgctgatgt cgttgacgat
     3061 cgaggcaccg gcgcggagcg ccaggtccgc aatcgaggct ttatacgtat ccaccgagag
     3121 tggaacctgc acccgcgcgg ccagcccttc gatgacgggc aggatgcgcc ggcgttcgtc
     3181 gtcggcgcca acggggcgcg cgccgggccg cgtcgattcg ccgccgatgt cgagcacgtc
     3241 ggccccctcc tcgaccatcc gcacgccggc atcgaccgcg cgtgccggat ccagcagccg
     3301 ccccccgtcg gagaaggagt cgggagtgac gttgatgatc cccatgacga gcgtgcgccc
     3361 accca
//
LOCUS       DBNGSH010000038         7044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2549455, whole genome shotgun sequence.
ACCESSION   DBNGSH010000038 DBNGSH010000000
VERSION     DBNGSH010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2549455"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="AC16CY_00760"
     CDS             <1..730
                     /locus_tag="AC16CY_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352332.1"
                     /translation="GDSIKPALAVVDTSLPSATGTRRADLLAHTGWATFLLWRDGDRQ
                     LNPADVYREALSIDPGNPYANAMLAHWTLFQDREDVPAAVKLFDTAVKAGRALDDVRT
                     LQWAAFGNTSAPEAAAERVRLADAMRRGGEKLDGRQASALWAPYYFATPAAREKERQL
                     LLDALPPDDHISTLSWAFDEYASRDEFRRRTIRYYVALLHEKASRVEQAVGDLRTLDK
                     ELAETRGSESLRDAVRAALQRLKR"
     gene            763..1815
                     /locus_tag="AC16CY_00765"
     CDS             763..1815
                     /locus_tag="AC16CY_00765"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3352333.1"
                     /translation="MSGRALSFLAVVIGAFLGTPRATAHHSFAPHFDSSKPVSISGAV
                     TEYEARNPHSYLHIAAVDENGRTQEYVCESHGVTQLTRNGIKPEMFKAGTKVRVTGSQ
                     SRHSRYMCFFDNVEFEDGRKLSVNGPGGAARPPAASLPPRKDIFGVWLLAPIPNRSTS
                     GPQPMTQFLTPAGEKAVAAYDPFKDDPTFRCDPVAIRRVWGAPGTPLEIARSGSDIVL
                     RHEWMDVRRVVHMNMKDHPKNGARGSLGHSIGRFDGDTLVIDTANYSAGVLNQYVEQP
                     GQPTRGLLHSAALTSVERLFLDAARQRLVVEVDLTDSEFFTRPFTRARLEYAPSDLKI
                     EPFNCSPEGVTGTIRK"
     gene            1865..3004
                     /locus_tag="AC16CY_00770"
     CDS             1865..3004
                     /locus_tag="AC16CY_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR3352334.1"
                     /translation="MAIAEKPKGGLEDVVATSSAICFLDGERGVLAYCGYDIHDLARS
                     ATFEEVCHLLWHRRLPSRAELGDLQSQLAAGRALPEPIIRLMRSLPPADGMDALRTIT
                     SALAHYDPEAADASPQAQYRKAVRLTAQVGSIVATWGRLQAGGGPISPDPAMGHAANF
                     LYMLSGQRPNSTAIRAFDVALVLHADHELNASTFAARVAAATLSDIYSAVVAGIGTLK
                     GPLHGGANADVMRMLLELGQTATADRVDEVIRGKLARKEKIPGFGHRVYRTEDPRATH
                     LRQMSHDLGKRSGNTAWFDMSQRIETLVKAEKKLNPNVDFYSATTYYTLGIPIDLYTP
                     IFAVSRMSGWTAHILEQYTNNRLIRPRADYTGPSYPQRFVPLDER"
     gene            3009..5426
                     /locus_tag="AC16CY_00775"
     CDS             3009..5426
                     /locus_tag="AC16CY_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3352335.1"
                     /translation="MGSTRLRAGLVAAVACVAAWASTVSRAEQRVAAPAAAPPAGELQ
                     LAITRYCAGCHNERSTTSAIASGVLLDKVDLAQVAERGAMWEKVVRKLRAGVMPPAGM
                     PRPDPDTHDALVTFLEDALDRHAAAHPNPGRHLPHRLNRVEYQNTIRDLLALDVDAST
                     LLPPDDSADGFDNNADVLGVSPALLERYLSAAAKISAIAVGSPSIGASSETYRIRGDA
                     SQTGQTEDLPPGTRGGLAALHTFPLDGEYVIKVKLLEINLGAIRGLEYQHQLEVAVDG
                     ERVLLAPVGGPEDYTESSLNAANVVNSLAARLQVRVQVRAGQRTVAAAFLQKSPAQGA
                     NRLQNFQRSTLIATETLGLPHVEHVTVSGPFNPTGVSSTPSRERLFVCRPRPGGGAKD
                     ERGCARRIVSTLARRAYRRPVTDEDLSSLMTFYESGHRDGGFERGIEMAVRAVLVSPK
                     FVFRIERDPEGVAPGTPYRVSDLELASRLSFFLWSSIPDDELLEEASRGRLRKPAALD
                     RQVRRMLADPRARALVSNFAGQWLHVRNLRNATPDKNDFPDFDDNLRRAFERELQLFV
                     GSIVAEDRSALELLTADYTFVNERLAKHYRIPNVYGPDFRRVAVSEDARKGLLGKGGI
                     LLVTSHADRTSPVLRGKWILDNVLGTPPPPPPEAVPPFPEETPGAPRSVRARMEQHRA
                     SPACAGCHRIMDPIGLALENFDAVGAWRTHDAGAAIDASGELADGSRVDGAVALREAL
                     LKRPEVLVGTMTEKLLTYALGRTLEHDDMPAVRAIVRASASDNYRFSSLVRGIVTSVP
                     FQMRRAD"
     gene            5427..6740
                     /locus_tag="AC16CY_00780"
     CDS             5427..6740
                     /locus_tag="AC16CY_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3352336.1"
                     /translation="MFLSRRSIPRRTFLRGMGAAVALPMLDAMVPALSAGAQGRPRPR
                     VGFVYIPHGVIMGQWTPAAEGAAFEFTTILKPLEPFRDSLVVVSNLTRAEPNSNHAVS
                     SACWLTGVPPKRTDGPDFRVGVSVDQVIARQIGQDTTFPSLEVATEDFTGLLGACDPG
                     YSCAYMNTLNWQTPTTPLPMEINPRVVFERLFGGGATADERLARMRTDRSLLDFVAED
                     LKQLERGLPAKDRARLGEYLDHVREIERRIERAEQRARMQPDVPTAPVGVPESYVEHV
                     DLLFDLLALAFQADLTRVFTFMMAREVSQRTYPEIGVTEPHHSISHHANRPAAIEGHT
                     KVNQYHVSMLARFLDRLRSIPDGDGSLLDHSIIVYGSGMSDGNGHTGAPLPHVLIGGG
                     AGRMTGGRHVVTREGTPMANLLLTVAQKCDVEQERFGVSTGTIAI"
     gene            6746..>7044
                     /locus_tag="AC16CY_00785"
     CDS             6746..>7044
                     /locus_tag="AC16CY_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3352337.1"
                     /translation="MTLTRAVGACVLLQIAATIVGVAAGSTPLIDAVRSGDGQAVTRL
                     LRQRTAVDSAEPDGSTALHWAARLDRAEIVQALLRSGAQAGRANRYGITPLTLAAT"
BASE COUNT         1161 a         2432 c         2381 g         1070 t
ORIGIN      
        1 cggcgactcc atcaagccgg cgcttgcagt ggtggacacc tcgcttccct ccgccaccgg
       61 gactcgtcgg gccgacctgc tggctcacac cggatgggcc acgttcctgc tgtggcgcga
      121 cggcgacagg cagttgaatc ctgcggacgt gtatcgggaa gccttgtcca tcgatccagg
      181 gaatccatat gcgaatgcca tgctggccca ctggactctc tttcaggacc gcgaggacgt
      241 cccggcagcg gtgaagctgt tcgacacggc cgtgaaagct ggccgtgccc tcgacgacgt
      301 gcgtaccctg cagtgggccg cgttcggcaa caccagcgcc cccgaggcgg ccgccgagcg
      361 cgtgcggctg gccgacgcga tgcgccgggg cggcgagaag ctggacggca ggcaggcgtc
      421 ggcgctctgg gctccttact acttcgcgac gccggccgct cgcgaaaagg aacggcagct
      481 gctgctggat gccctgccgc ccgacgatca catcagcacg ctgagctggg cgttcgacga
      541 gtacgcatcg cgcgacgagt tccgtcggcg cacgattcgg tactacgttg cgctgctgca
      601 cgagaaggcc tcacgggtcg agcaggccgt cggcgatctg cggacgctcg acaaggagct
      661 cgccgagact cgcgggtccg agtcgctgcg cgacgccgtg cgcgcggcgc tccagcgcct
      721 gaagcgctga tggttttata ctcacgcatc agggagggtg gcatgagcgg acgggcgctt
      781 tcgttccttg cggttgtcat cggcgcattc ctcggcactc cgcgagcgac cgcccatcac
      841 agcttcgcgc ctcacttcga ctcgagcaag ccggtcagca tctcgggcgc cgtcaccgag
      901 tacgaggcgc gcaatccgca cagctacctt cacattgccg ccgtggacga gaacggcagg
      961 acgcaggaat acgtctgcga atcgcacggc gttacccagc tcacacgcaa cggaatcaag
     1021 cccgagatgt tcaaggccgg cacgaaggtc cgcgtcacgg gctcccagtc caggcacagt
     1081 cgctacatgt gcttcttcga caacgtcgag ttcgaggatg gccgcaagtt gagcgtcaac
     1141 ggtcctggcg gcgcggcacg gccgcccgca gcgtctctgc cgccgcgcaa ggacatcttc
     1201 ggcgtctggc tgctggcgcc gatcccgaac cggagcacga gcggcccgca gccgatgacg
     1261 cagttcctga cgccggccgg tgagaaggcc gtggccgcct acgatccgtt caaggacgat
     1321 ccgacgttcc gctgcgaccc ggtcgcgatc cgccgcgtct ggggcgcacc cggcacgccg
     1381 ctggagatcg cccgcagcgg cagcgacatc gtgctgcgtc acgagtggat ggacgttcga
     1441 cgcgtcgtcc acatgaacat gaaggaccat ccgaagaacg gcgcgcgagg ctcgctcggc
     1501 cactcgatcg gccgcttcga cggcgacacg ctggtcatcg acaccgcgaa ctactccgcg
     1561 ggcgtgctga accagtacgt cgagcagcca ggccagccga ccagggggct gctgcactcg
     1621 gcggcgctca cgtcggtcga gcggctgttc ctcgacgccg cgcgccagcg tctcgtcgtc
     1681 gaagtcgatc tgacggactc ggagttcttc accaggcctt tcacgcgcgc gaggctggag
     1741 tacgccccgt ccgatctgaa gatcgagccg ttcaactgct cgcccgaggg cgtgacgggg
     1801 acgattcgca agtagtacgc agggggcagg gggcaggggg cagggggcag agtaagatcg
     1861 cttcatggcc attgcggaga agcccaaggg cggcctcgaa gacgtcgtcg cgacctcctc
     1921 ggcgatctgc ttcctggacg gggaacgggg agtgctcgcc tactgcggat acgacatcca
     1981 cgacctggcg aggtcggcga cattcgagga agtctgtcat ctgctgtggc atcgccggct
     2041 gccttcgcgt gccgagctgg gtgacctgca gtcgcagctg gcggccggca gggcattgcc
     2101 cgaaccgatc atccggttga tgcgcagcct gccgccggcc gacggcatgg acgcccttcg
     2161 cacgatcacg tccgcgctgg cgcactacga cccggaagcc gctgacgcgt ctccgcaagc
     2221 gcaataccgg aaggccgtca gactgaccgc gcaggtcggc tcgatcgtgg ccacgtgggg
     2281 ccgcctgcag gccggcggcg gtcccatctc gcccgatccc gcgatgggtc acgccgcgaa
     2341 cttcctgtac atgttgagcg ggcagcggcc gaactcgacg gcgatccgcg cgttcgacgt
     2401 cgcgctcgtc ctgcacgcgg atcacgagtt gaacgcctcg accttcgccg cccgcgtcgc
     2461 ggcggccact ctcagcgaca tctactcggc cgtggtcgcg ggcatcggca cgctgaaggg
     2521 ccccctgcac ggcggcgcca acgcggacgt catgcgaatg ctcctggagc tgggacagac
     2581 ggccaccgcc gatcgcgtgg acgaggtcat tcgcggcaag ctcgcacgca aggagaagat
     2641 cccggggttc ggtcaccggg tctacaggac cgaggacccg cgcgccacgc atctgcggca
     2701 gatgtcgcac gacctcggca agcgttcggg caacaccgcc tggttcgaca tgtcccagcg
     2761 gatcgaaacg ctcgtgaagg ccgagaagaa gctcaatccc aacgtcgatt tctactcggc
     2821 gacgacgtac tacacgctcg gaatcccgat cgatctgtac acgcccatct tcgccgtcag
     2881 ccggatgtcg ggatggacgg cgcacatctt ggagcagtac acgaacaaca ggctgatccg
     2941 tccgcgcgcc gattacaccg ggccgtccta cccccagcgg ttcgttcccc tcgacgagcg
     3001 gtaagaggat gggatcgacg aggcttcgag caggtctcgt ggcggccgtg gcatgcgtgg
     3061 cggcgtgggc ttcgaccgtg tcacgggccg agcagcgcgt cgccgcacca gcggcagcac
     3121 cgccggccgg cgagctgcag cttgcgatca ccaggtactg cgccggctgt cataacgaac
     3181 gcagcacgac ctccgccatc gcctctggcg tgctcctcga caaggtggac ctcgcacagg
     3241 tggccgagcg cggcgcgatg tgggagaagg tcgtccgcaa gctccgcgcg ggcgtgatgc
     3301 ccccggccgg catgcccagg cccgaccccg acacgcacga cgcgctcgtg accttcctcg
     3361 aggacgcgct ggatcgtcac gcggctgcgc acccgaaccc ggggaggcat ctgccgcacc
     3421 ggttgaatcg tgtcgagtac cagaacacga ttcgcgatct gctggctctc gacgtcgatg
     3481 cctccactct gctgcccccg gacgactcgg ccgacgggtt cgacaacaac gccgacgtgc
     3541 taggcgtctc tcccgcgctg ctcgagcggt acctgtcggc ggcggcaaag atcagcgcga
     3601 tcgccgtcgg cagcccgtcg atcggcgcga gctcggaaac ctatcgcata cgcggggatg
     3661 catcgcagac agggcagacc gaagatctgc ctcccgggac tcgaggcggg ctggccgcgc
     3721 tgcacacgtt cccgctcgac ggcgagtacg tcatcaaggt gaagctgctc gagatcaacc
     3781 tcggggcgat tcgcggcctc gagtaccagc accagctcga ggtggccgtc gatggcgagc
     3841 gcgtcctgct tgccccggtt ggcggcccgg aggactacac cgagtcgtcg ctcaacgccg
     3901 ccaacgtcgt gaactcgctg gcggcgcggc tgcaggtgcg cgtccaggtg cgcgccggcc
     3961 agcgcacggt cgccgccgcg ttcctccaga agagccccgc gcagggagcg aaccggctgc
     4021 agaatttcca gcggagcacg ctcatcgcca ccgagactct cggcctgccg cacgtggagc
     4081 acgtcacggt cagcggcccc ttcaacccga cgggcgtgag cagcacaccc agccgcgagc
     4141 gcctgttcgt gtgtcgccct cgaccaggcg gcggcgcgaa ggacgaaagg ggatgcgcga
     4201 ggagaatcgt cagcaccctg gcgcgccgcg cgtatcgccg gcccgtgacc gacgaggacc
     4261 tgtcgagcct gatgacgttc tacgagtccg gccatcgcga tggcggtttc gagcgcggca
     4321 tcgagatggc ggtgcgcgcc gtgctcgtga gcccgaagtt cgtcttccgt atcgagcgcg
     4381 acccggaagg cgtcgcgccc ggaaccccgt accgcgtgag cgatctcgag ctcgcctcgc
     4441 ggctctcctt cttcctctgg agcagcatcc ccgacgacga gctgctcgag gaggcgagcc
     4501 gcggccggct gcgcaagccc gctgcgctgg atcggcaggt ccgccgcatg ctggccgacc
     4561 cgcgtgcccg ggcgctcgtc tcgaacttcg ccgggcagtg gctgcacgtg cgcaacctga
     4621 gaaacgcgac gcccgacaag aacgacttcc cggacttcga cgacaacctg aggcgggcct
     4681 tcgagcgcga gctgcagctg tttgtcggca gcatcgtcgc cgaggatcgc agcgcgctgg
     4741 agctgctgac ggccgactac acgttcgtga acgagcggct cgcgaagcac taccgcatcc
     4801 cgaacgtcta cggcccggat ttccgtcggg tcgccgtgtc cgaggacgca cggaaggggc
     4861 tgctcggcaa aggcgggatc ctgctcgtga cctcccacgc cgatcgcacg tcaccggtcc
     4921 tgcgcggaaa gtggatcctc gacaacgtgc tcggcacgcc tccgcctcca ccgcccgagg
     4981 cggtaccacc cttccccgag gaaacgccgg gagcgcccag gtcggttcgc gcccggatgg
     5041 agcagcatcg cgcgagcccg gcgtgcgcgg gctgccaccg tatcatggac ccgatcgggc
     5101 tggcgctcga gaacttcgac gccgtgggcg cctggcggac gcacgacgca ggcgccgcca
     5161 tcgacgcctc cggcgagctg gcggacggaa gccgcgttga cggcgcggtc gccctgcgcg
     5221 aggcgctctt gaaacgcccc gaggtgctgg tcggcacgat gacggagaag ctcttgacct
     5281 acgcgctcgg ccgcacgctg gagcacgacg acatgccggc cgtccgggcg atcgtccgcg
     5341 catcggcgag cgacaattac cgcttctcgt cgctcgtccg cgggatcgtg acgagcgtac
     5401 cgtttcaaat gaggagagcc gactagatgt tcctctcgcg acggtccatt ccccggcgaa
     5461 ccttccttcg cggcatgggc gccgccgtag cgctgccgat gctggacgcg atggtgccgg
     5521 cgctgtctgc cggggcccag ggccggccca ggccccgcgt cggcttcgtc tacatcccgc
     5581 acggcgtcat catggggcag tggactccgg ccgccgaggg cgcggccttc gagttcacca
     5641 cgatcctcaa gccgctcgag ccgtttcgcg actcgctcgt cgtcgtcagc aacctcacgc
     5701 gggccgaacc gaacagcaac cacgcggtgt cgtcggcgtg ctggctgacc ggcgttccgc
     5761 ccaagcggac cgacggcccc gacttccgcg tcggcgtctc ggtcgatcag gtcatcgcgc
     5821 ggcagattgg ccaggacacg acgttcccct cgctcgaggt cgccaccgag gacttcacgg
     5881 gcctgctcgg cgcctgcgat ccgggataca gctgcgcgta catgaacacg ctcaactggc
     5941 agacgccgac aacgccgttg cccatggaga tcaacccgag agtcgtgttc gagcggttgt
     6001 tcggcggcgg tgcgacagcc gacgagcggc tggcgaggat gcggaccgac cggagcctgc
     6061 tcgacttcgt tgccgaggac ctgaagcaac tggaacgagg ccttccggcg aaagaccgcg
     6121 cacgcctcgg cgagtatctc gatcacgtcc gggagatcga gcggcggatt gagcgcgccg
     6181 agcagcgggc gcggatgcag ccggatgtgc cgaccgcgcc tgtcggcgtg ccggagtcct
     6241 acgtcgagca cgtggatttg ctgttcgacc tgctggcgct ggcgttccag gcggatctga
     6301 cgcgtgtgtt cacgttcatg atggcgcgcg aggtgagcca gcgcacctac ccggagattg
     6361 gcgtcaccga gccgcatcac tccatctcgc accacgccaa ccggccggca gccatcgagg
     6421 ggcacaccaa ggtcaaccag taccacgtga gcatgctggc caggttcctg gatcggctgc
     6481 ggtcgattcc tgatggcgac ggctcgctgc tcgatcattc gatcatcgtg tacggcagcg
     6541 gcatgagcga cgggaacgga cacaccggcg cgccgttgcc gcacgtgctg atcggcggcg
     6601 gggctggacg gatgacgggc ggccgccacg tcgtgacgcg cgaaggcacg ccgatggcca
     6661 acctcctgct gacggtggcg cagaagtgcg atgtcgagca ggagcgcttt ggcgtgagca
     6721 ccggcaccat cgcgatctaa cggacgtgac tctcacccgc gccgttggcg cctgcgtgct
     6781 tcttcagatc gccgcgacaa tcgtcggcgt ggcggcggga agcacgccgc tcatcgacgc
     6841 cgtgagaagc ggtgacggtc aggcggtcac gcggctgctc cggcagagaa ccgcggtgga
     6901 ttccgcagaa ccggatggat cgacggccct gcactgggcc gcccgtctcg accgcgccga
     6961 gatagtgcag gcactcctgc ggagcggcgc gcaggcgggg cgggccaacc gttacgggat
     7021 cacgccgctg acccttgccg ccac
//
LOCUS       DBNGSH010000039         2334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_851497, whole genome shotgun sequence.
ACCESSION   DBNGSH010000039 DBNGSH010000000
VERSION     DBNGSH010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_851497"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="AC16CY_00790"
     CDS             complement(<1..500)
                     /locus_tag="AC16CY_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024769.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR3352338.1"
                     /translation="MTGKTDKPQGPGPRPQVRPTSRAAWRKWLEEHHATSRGVWLVFA
                     KKHTKLPSLSYDEAVEEALCFGWIDGRAGSLDDDAYLLLMTRRKPGSGWSAINKERIE
                     RMVAAGLMTPAGQAVIDAAKADGSWARLDASESMAIPEDLATALRAYPDAERHFDAFP
                     PSARKI"
     gene            complement(481..1224)
                     /locus_tag="AC16CY_00795"
     CDS             complement(481..1224)
                     /locus_tag="AC16CY_00795"
                     /inference="COORDINATES: protein motif:HMM:NF039411.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HIR3352339.1"
                     /translation="MATRKAGRASKKKGAARRRAGGGARKAAKKRASAKKRAAPRARK
                     PVRRAKVRTASRKAAKRVAKRRVRRPKVVARRRVAAPKSRRRVVKKTTQIAPARSSAA
                     PPARRAAPPKRVVRTAPGLDRERRRISDDEFVPTPPSSLDMDRSASAARSGRRELREK
                     YDEHTETGPAMTGGDIDADWESAYSVGDEAPGGDNPTPDQDIVDDIGKAVGVQYNDNE
                     ELKGEKKITERDRHRWELDPASSDDWEDR"
     gene            1577..>2334
                     /locus_tag="AC16CY_00800"
     CDS             1577..>2334
                     /locus_tag="AC16CY_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR3352340.1"
                     /translation="MTNGDDGLVSPESAGGQPMAPGRTRSGKPAGPAFKPMHQEDMDP
                     AEFTSMLEAYDDSFRNMAEGEVVKGTVLKVTEAAVVVDVGYKSEGLIPIHEFLDESGN
                     VTVQAGDTVDVLLERTEDREGHIVLSREKAEKMKIWDEVEKAYADRKVVIGRVIERIK
                     GGLAVDIGVRAFLPGSQIDVRPVRNLDALKGQELRMRVIKVNKKRGNIVLSRKALLEE
                     ENAEKKKTTLETLSEGKVLRGTVKNITDYGAFIDL"
BASE COUNT          382 a          769 c          780 g          403 t
ORIGIN      
        1 atgatcttcc gcgctgaggg cgggaaggcg tcgaagtgac gctcggcgtc cgggtaggcg
       61 cgcagcgccg tcgcgaggtc ctccgggatc gccatcgact cgctcgcatc cagcctcgcc
      121 cacgatccgt ccgccttcgc ggcgtcgatc accgcctggc cggcgggcgt catcagcccg
      181 gcggcgacca tccgctcgat ccgctccttg ttgatcgccg accagccgct cccgggcttg
      241 cgccgggtca tcagcagcag gtacgcgtcg tcgtcgagcg atccggcccg cccgtcgatc
      301 cagccgaagc acagggcctc ctcgacggcc tcgtcgtagg agagcgacgg gagcttcgtg
      361 tgcttcttgg caaagacgag ccagaccccg cgggacgtag cgtgatgctc ctcgagccac
      421 ttacgccagg ctgccctcga tgtgggtcga acctgaggcc tgggccctgg gccctgaggc
      481 ttatctgtct tcccagtcat ccgacgatgc aggatcgagc tcccagcggt ggcgatcgcg
      541 ctcggtgatc ttcttctcgc ccttgagctc ctcgttgtcg ttgtactgca cgccgacggc
      601 cttgccgatg tcgtcaacga tgtcctggtc cggcgtcggg ttgtctccgc cgggcgcttc
      661 gtctccgacg ctataggccg attcccaatc ggcgtcgatg tcgcctccgg tcatcgcagg
      721 accggtctcg gtgtgctcgt cgtacttctc gcgcagctcg cggcgccccg accgggccgc
      781 cgacgcggac cgatccatgt cgagcgacga cggtggcgta ggcacgaact cgtcgtcgct
      841 gattcgccgc cgctcgcggt cgagacctgg cgccgtccgc accactctct ttggcggggc
      901 cgctcgtcgt gcagggggcg cagcggagga gcgggcaggc gcgatctgcg ttgtcttctt
      961 gacgactcgc cgccgcgact tgggggcggc gacgcggcga cgggcgacga cctttggccg
     1021 gcgcacgcgg cgtttcgcga cgcgcttggc ggccttccgg ctggccgttc tgaccttggc
     1081 ccggcggacc ggctttctcg cacgcggggc ggcgcgtttc ttcgccgatg ccctcttttt
     1141 cgctgccttt cgggccccgc cgccggcgcg acgacgggcg gcgcccttct tcttcgaggc
     1201 tcgtccggcc ttccgtgtgg ccattgtcag atacctccga accttgacag taaccgataa
     1261 gttacgtaga ctctaatgct tatcggcgac ggcgggcccc cggcaggggg agcgggacgc
     1321 ggcctcgggt cgccttacct gcgtgcagca ccctccacga gaatcgccat cgccgttata
     1381 tccgcggggg gccggtcccc gcaatccctc gattcactcg ggattgaccc gagcgacggc
     1441 gagggatgac cgttcgattc tactcaggtt gctctgagcc acgtcgaaag gcgaaaaaaa
     1501 accggcagcc caggaggcgc gatccgcacg cggacgcagg ccgccgggca tcacgaatga
     1561 ggaggacgcg cgcgtaatga cgaacggaga cgacgggttg gtatcgcccg aatctgccgg
     1621 cggacagccc atggcgcctg ggcggacacg ctcgggcaag ccggctggcc ctgccttcaa
     1681 accgatgcac caggaggaca tggaccctgc ggagttcacg tccatgctcg aggcctatga
     1741 cgacagcttc cgcaacatgg cggaggggga ggtcgtcaag gggacggtgc tgaaagtcac
     1801 cgaggcggcg gtcgtcgtcg acgtcggcta caagtcggaa ggcctcatcc ccatccacga
     1861 attcctcgac gagagcggca acgtgacggt gcaggccggc gacacggtcg acgtcctgct
     1921 cgaacgcacc gaggaccgcg aaggccacat cgtgctatcg cgcgagaagg ccgagaagat
     1981 gaagatctgg gacgaagtcg aaaaggccta tgccgatcgc aaggtcgtca tcggtcgcgt
     2041 gatcgaacgc atcaagggtg gtctcgcagt cgacatcggc gtccgcgcgt tccttcccgg
     2101 atctcagatc gatgtgcgac cggtgcggaa tctggatgcg ctgaaggggc aggagctccg
     2161 catgcgcgtc atcaaggtca acaagaagcg cggcaatatc gtcttgtcgc ggaaggcgct
     2221 cctcgaagag gaaaacgcgg aaaagaagaa gaccaccctc gagacgctgt cagaggggaa
     2281 ggtgttgcgc gggacggtga agaacatcac cgactacggc gcgttcatcg acct
//
LOCUS       DBNGSH010000040         2761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1870512, whole genome shotgun sequence.
ACCESSION   DBNGSH010000040 DBNGSH010000000
VERSION     DBNGSH010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1870512"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16CY_00805"
     CDS             complement(<1..473)
                     /locus_tag="AC16CY_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3352341.1"
                     /translation="MTAVGYKRIATEEAWAPMELLDTYRSMAAKKSIDDPGFIALWTR
                     LGARPVLSERLADIGERRIADMDSSGIDLQILSLTSPGVQVFDAPTAASLASSTNDQV
                     AEAVRKYPTRFAALAAVAPQDPRGAAREAERAVRRLGLKGLIINSHTRGEHLDDP"
     gene            complement(521..1564)
                     /locus_tag="AC16CY_00810"
     CDS             complement(521..1564)
                     /locus_tag="AC16CY_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352342.1"
                     /translation="MVPREYLLPYLISNLVALAILGLAFWRPRIVKWTWVVIFLWAAG
                     MNTLTATREPWQYLLYGALTPSDTYRAFIDGWFSRHVTQMVLSIAVGQVAIAFLLARA
                     ESRRLGVLGASVFLLAIAPLGVGSAFPFSLTAIASLLVMEWRLGALRRLESSPASRFI
                     PREDTREDHSIDIQAPAHVVFEVAKHLDLLSDPLYVALVRIRAFVMGDQPRTRTASGL
                     VAEMLALGWGVLSYSAGRTLVMGAVARPWRRDVTFTAVDPCEFTSFAEPDLVKIVWTL
                     EVEPLGPALTRFRSETRTKATDPTTRRKFLWYWRAVSPGVRLIRWRILRALRREAERC
                     YRAERVEARRQAA"
     gene            complement(1711..1986)
                     /locus_tag="AC16CY_00815"
     CDS             complement(1711..1986)
                     /locus_tag="AC16CY_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HIR3352343.1"
                     /translation="MAKKKKSARKPSAAFMRPVSPSPALAEVIGSKAVPRTEVTKRLW
                     AYIKKHKLQDAKNRRMINADAALKAVFGGRSSVNMFEMTKLVGKHLK"
     gene            2112..>2761
                     /locus_tag="AC16CY_00820"
     CDS             2112..>2761
                     /locus_tag="AC16CY_00820"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3352344.1"
                     /translation="MASPLFQIRDLAKVYHMGEVEVHALRRVTLGLDEGQFVVLLGPS
                     GSGKSTLLNIIGGLDVPTSGQVLYRDTDLTRADEAALTAYRREHVGFVFQFYNLIPSL
                     TAVENVALVTEISDNPMAPEEALGLVKLEERLDHFPAQLSGGEQQRVAIARAIAKRPS
                     VLLCDEPTGALDISTGIVVLEALQRVNADLGTTTVVITHNAAIASMADRVIRLADGR"
BASE COUNT          478 a          929 c          934 g          420 t
ORIGIN      
        1 ttcgggtcgt ccagatgctc gcctcgcgtg tgcgagttga tgatgaggcc cttcagcccg
       61 agccgccgga cggcgcgctc cgcctcgcgc gcagcgcctc gaggatcctg cggggccacg
      121 gcggccaaag ccgcgaagcg ggtcgggtac ttccgaacgg cctcggccac ctgatcgttg
      181 gtggacgatg caagagaggc ggcggtcggg gcgtcgaaca cctgcacgcc gggcgaggtg
      241 agcgacagga tctgcaggtc gatgcccgag ctgtccatgt cggcgatgcg gcgttcgccg
      301 atgtctgcca gccgctcgga cagcaccggg cgcgcgccca gccgcgtcca gagcgcgatg
      361 aagcctggat cgtcgatcga cttcttcgcc gccatggagc ggtaggtgtc gagcagctcc
      421 atgggcgccc acgcttcttc ggttgcgatt cgtttgtatc cgactgccgt catcacgcct
      481 cccgcggccc gccagaaccg cgaaggcgat gctaacccaa tcaggccgcc tgacggcgcg
      541 cttccacgcg ctcggcacgg tagcaacgct cggcctctcg ccgcagcgcg cgcagaatgc
      601 gccaccggat gaggcgaacg ccggggctca ccgcgcgcca gtaccaaagg aacttcctgc
      661 gcgtcgtggg gtcagtggcc ttcgtccgcg tttcagagcg aaatcgggtc agcgccgggc
      721 cgagcggctc cacttcgagg gtccagacaa tcttgacgag atcgggctcg gcgaacgacg
      781 tgaactcgca cggatcgacg gcggtgaacg tgacgtcccg cctccagggg cgcgcgaccg
      841 cgcccattac cagcgtccgg ccggccgagt aggagaggac gccccaacca agcgcgagca
      901 tctcggcgac gagcccgctg gccgttcgcg tcctgggctg atcgcccatc acgaaggcgc
      961 gaatccggac aagcgcgaca tacagaggat cggacaggag gtccagatgt ttggccacct
     1021 cgaagaccac atgggccgga gcctggatgt caatcgagtg gtcctcccgt gtgtcctcgc
     1081 ggggaatgaa ccgcgatgcc ggtgaagact ccagacgcct cagagcgccg agcctccact
     1141 ccatcaccag cagcgaggcg atcgccgtca gggagaacgg gaaggcggac ccgactccca
     1201 ggggagcgat cgccaggaga aacaccgagg cgccgagcac gccgagccgc cggctctcgg
     1261 cccgggcgag caggaaagcg atggccacct ggccgaccgc gatcgacaac accatctggg
     1321 tgacgtgccg actgaaccag ccgtcgatga acgcgcgata ggtgtcggac ggcgtcagcg
     1381 cgccgtagag caggtactgc cagggctccc tggtcgcggt cagcgtgttc atcccggccg
     1441 cccagaggaa gatgaccacc caggtccatt tcacgattcg cggccgccag aacgccaggc
     1501 caaggatggc gagcgcgacg agattggaga tgagatacgg gagaaggtat tcgcgaggaa
     1561 ccatgggacc cctcctgcac tgtcgtgctg acagcaaaga gggcaagccc cgggccgtac
     1621 gtgaggtggc gattggccgg agatgtgcgg gcgcggcgct cggatagccg ggaagaatcc
     1681 gcagcgccgc ggcattatcc gcgcgggtgc ctacttcaga tgcttgccga ccagcttcgt
     1741 catctcgaac atgttcaccg aggaccggcc gccgaacacg gccttcagcg cggcgtcggc
     1801 gttgatcatc cggcggttct tcgcgtcctg cagcttgtgc ttcttgatgt aggcccagag
     1861 gcgcttggtc acctccgtcc gcggaaccgc cttggagccg atcacttcgg cgagcgcggg
     1921 gctgggggac acgggcctca tgaaagccgc actcggcttc ctcgcggact tcttcttctt
     1981 tgccatctgg cctccttgga agcgtacgtc acactgtgca ggactgccgg atggtagcac
     2041 gcccgtccgc cgggccaact cacggccgcg ggctggacac gcgccggcca ctcgacacac
     2101 gcttgacaga gatggcatcc cccctcttcc agatccgcga cctggccaag gtctatcaca
     2161 tgggcgaggt tgaggtgcac gcgctgcggc gcgtgacgct cggcctcgac gaggggcagt
     2221 tcgtggtcct gctggggccc tccggctcgg gaaaatcgac gctgctcaac atcatcggcg
     2281 gactggacgt gccgacgagc gggcaagtgc tctaccggga caccgatctg acgcgcgcag
     2341 atgaggcggc gctcaccgcg taccggcgcg agcacgtcgg cttcgtcttc cagttctaca
     2401 acctgattcc gagcctcacc gcggtcgaga acgtcgcgct cgtcaccgag atctccgaca
     2461 acccgatggc gccggaggag gcgctcgggc tcgtgaagct cgaggagcgt ctcgaccatt
     2521 tcccggcgca gctctcggga ggcgagcagc agcgggtggc catcgcgcgc gccatcgcga
     2581 agcgtccgtc cgtgctcctc tgcgacgagc ccacgggcgc cctcgacatc tccacgggga
     2641 tcgtcgtgct cgaggcgctg cagcgcgtca acgcggacct ggggacgacg acggtcgtga
     2701 tcacgcacaa cgcggcgatc gcttcgatgg ccgaccgcgt catccggctc gcggacggcc
     2761 g
//
LOCUS       DBNGSH010000041         2434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_172481, whole genome shotgun sequence.
ACCESSION   DBNGSH010000041 DBNGSH010000000
VERSION     DBNGSH010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_172481"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..1263)
                     /locus_tag="AC16CY_00825"
     CDS             complement(319..1263)
                     /locus_tag="AC16CY_00825"
                     /inference="COORDINATES: protein motif:HMM:NF017536.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3352345.1"
                     /translation="MTKPLVAAESLLAIGATERRLEAYANYDIMLGALGISRALPVKG
                     KRMIGPWCFLDRFGPLSFTEGMPMDVAPHPHMGLQTVTWLLEGEIVHDDSLRQEALLR
                     PGGVNVMTSGRAIAHAERTPTDNTGRLNGVQLWTALPDRDRHSDPSFQHLPDVPRLEH
                     PGGIIRVFSGRIAEAVSPARHFSDLLGADVQVHAGETMTLPLQAGYEHGLLILAGDCA
                     FEGQALEPRVLYYLGTQRTDAALRSARGARILLVGGPPFPETILMWWNFVARTPEEIR
                     DARDDWEGRRRFGDVPAYKGPRLAAPELVRLARPNPVS"
     gene            1287..>2434
                     /locus_tag="AC16CY_00830"
     CDS             1287..>2434
                     /locus_tag="AC16CY_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352346.1"
                     /translation="MRSSSRTILVYGLMLACVTVCCSRQAAIPRTADGRPDLQGIWQV
                     RNRASYNLEDHVARHGMPAGTGVVQGGPIPYQEWAAKRKIENAANRQNDPLANCYLPG
                     VPRIMYMEFPFQIFQTPDHIAMTFEWSQVHRTVYTNGVPAPEGIDFWMGDSRGRWEGD
                     TLVVEVSDHNDRTWFDMAGNFHSEELTLVERYTMVNADTIQYEVTITDPKVFTRPWTI
                     AMMLHRSEAARIFEYQCQAEAEEANGAFEPDPKTWYPGPGAPVTIPAEWKGDTGESAW
                     KPPARKGEIRRMPDGKPDLQGYYMPDGGGGNYGLGKHEQDFLTPGGRGIIVDPPDGIL
                     PMQAWAKLEAEDRKTPERGYDDPTAHCFVAGVPRSMYVPSPLQILQPPG"
BASE COUNT          440 a          800 c          813 g          381 t
ORIGIN      
        1 gcccgagctg gccaacgatc cggtatttgc cgagtgtcct gccaatcatc agagtcgcta
       61 atcagcgagc ggcgtgtcct ggcccggcct cgctcgaaaa tgcgcaccag cgcgcatggc
      121 tcctacacag actcgatttc tggagccgct gtttcaattt cggcccctcc tccagcatgc
      181 gatagacagg ccaaccatcg tccggactcg gccgcacggg atggcatggt tcgacacgcg
      241 aatcttgaat gtgactgcca gttattggca gctggaagtc agaaattggc agtcggaaat
      301 ggggtcggga gtcgtgtatc agctgaccgg attgggccgc gcgagccgaa cgagctcggg
      361 cgcggcaagg cggggaccct tgtaggcggg gacatcgccg aagcggcgac ggccctccca
      421 gtcatcgcgc gcgtcccgga tctcctcggg tgttcgcgcg acgaagttcc accacatgag
      481 gatcgtctcc gggaacggcg ggccgccgac gaggaggatg cgcgccccgc gtgcgcttcg
      541 cagcgccgca tccgtgcgct gcgtgccgag atagtagagg acgcgcggct cgagcgcctg
      601 cccttcgaag gcgcagtcgc cagcgaggat gagcagcccg tgctcgtagc cagcctgcag
      661 gggaagggtc atcgtctctc cggcgtgcac ctgcacgtcc gctccgagca gatcggagaa
      721 gtggcgtgcg ggcgatacgg cttccgcgat ccggcccgag aacacgcgga tgattccgcc
      781 gggatgctcg agccgtggga cgtcggggag gtgctggaac gacgggtccg agtgccggtc
      841 gcgatcggga agcgccgtcc agagctgaac gccgttcagc cgcccggtgt tgtcggtcgg
      901 cgtccgctcg gcgtgcgcga tcgcgcgccc cgacgtcatc acgttcacgc cgcccgggcg
      961 gaggagcgcc tcctgccgga ggctgtcgtc gtgcacgatc tcgccttcga gcagccacgt
     1021 caccgtctga aggcccatgt gcggatgcgg tgcgacgtcc atcggcatgc cctcggtgaa
     1081 cgagagcggg ccgaatcgat cgagaaagca ccacggaccg atcatgcgct tgcccttgac
     1141 gggcagcgcc cgcgagatgc cgagcgcgcc gagcatgatg tcgtagttgg cgtacgcctc
     1201 gagccgccgc tccgtggcgc cgatcgccag caacgattcc gccgcgacca acggcttcgt
     1261 cattcaggta cagtctacaa tgcgccatgc gctcgagttc ccgaacgatc ctcgtctacg
     1321 gtctgatgct cgcgtgtgtc accgtctgct gctcgcgtca ggcagcgatc ccgaggacgg
     1381 cagacggcag gcccgacctc cagggcatct ggcaggtccg caatcgtgct tcctacaacc
     1441 tcgaagacca cgtggcgcgc cacgggatgc ccgcggggac cggcgtcgtg cagggcggac
     1501 cgattccata ccaggagtgg gcagccaaga ggaagatcga gaacgcggcc aaccggcaga
     1561 acgacccgct tgccaactgt tacctgccgg gcgtgccgcg catcatgtac atggagttcc
     1621 cgttccagat ctttcagacg cccgatcaca tcgcgatgac cttcgagtgg tcgcaggtgc
     1681 atcgcaccgt ctacaccaac ggcgtgcccg cgcccgaggg catcgacttc tggatgggtg
     1741 actcgcgagg ccggtgggag ggagacacgc tcgttgtcga ggtaagcgac cacaacgacc
     1801 gcacgtggtt cgacatggct ggcaacttcc acagcgagga gctgacactg gtcgagcggt
     1861 acacgatggt gaacgccgac accattcagt acgaggtcac gatcaccgat ccaaaagtct
     1921 tcacgcggcc gtggacgatc gcgatgatgc ttcatcgctc ggaggcggcg cggatcttcg
     1981 agtaccaatg ccaggcggag gcggaagaag cgaacggcgc gttcgagcct gacccgaaga
     2041 cgtggtaccc gggcccggga gcgccggtga cgattcccgc cgagtggaaa ggcgacaccg
     2101 gcgaatcggc ctggaagcct cccgcgcgaa aaggcgagat caggcgcatg cccgacggca
     2161 agcccgacct gcagggctac tacatgcccg acggcggcgg cggcaactac ggtctcggca
     2221 agcacgaaca ggacttcctc acccccggcg gccgcggcat catcgtcgat ccgccggacg
     2281 gcattcttcc gatgcaggcg tgggcgaagc tcgaggccga ggatcgcaag actcccgagc
     2341 gcggctacga cgacccgacc gcccactgct tcgtggccgg cgttccgcga tcgatgtacg
     2401 tgccgtcgcc gctgcagatc ctccagccgc ccgg
//
LOCUS       DBNGSH010000042         2331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4417465, whole genome shotgun sequence.
ACCESSION   DBNGSH010000042 DBNGSH010000000
VERSION     DBNGSH010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4417465"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="AC16CY_00835"
     CDS             complement(<1..648)
                     /locus_tag="AC16CY_00835"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR3352347.1"
                     /translation="MGKAAPAKVERPGASGGMSVRRRALMLGLLVAALAVVASSTQLH
                     GWLVGFLPEAEALIRNRPVLGVTVFVLFAAASAMLAFVSSALIVPVGVYVWGKGISTM
                     LLLFGWILGGVVAYTIGRYLGRPAVKAITSNPLLDRYADRVSVKAPIGLVLLFQAALP
                     SEVPGYVLGLIRYSFWKYLGVLSLTELPYSVATIYLGAGFVERRITLLIVVGALMV"
     gene            complement(648..1643)
                     /locus_tag="AC16CY_00840"
     CDS             complement(648..1643)
                     /locus_tag="AC16CY_00840"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3352348.1"
                     /translation="MSGFSRTVIMMQARPRLIVLALGVVLASIVLGGQSQNPVPASVP
                     LAADAPFTVAVSTSTIEGGPVYVADNGPLGAGFRVINGGVRNLANNSAHAATNAETQF
                     LIAGAPNVRILFTVAEGLYRIVARRSAGIRTLADLRGKRVTLPRDTSAHYSLVRMLAS
                     AGLSESDVTIVDLPRGGMAAGLVQGAADAISMWEPEAQVALESLGPDAIVFQDNRIYR
                     ELFSLYTTTDVLNDSRRRRELVQFVRALLAAVDEMKTKPAGHFPLIAKTVNQPVDVVA
                     RSWPHHAFPVAVPADMLDIMVEEELWVARKQGRDPRSRATLASLFDASVLEEARR"
     gene            1739..>2331
                     /locus_tag="AC16CY_00845"
     CDS             1739..>2331
                     /locus_tag="AC16CY_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3352349.1"
                     /translation="MKRRAVLAAMVLLGAIGLPAAAQSPDGATVFQTACASCHAQPAP
                     DSRAPNRDTLAQLAPETILTALTTGNMFRQGTALTDAERRAVSAFIAGRPVGTPAPPS
                     TVGRCTTTPPPLRAADLVSGWNGWGTGTSNTRFQTAEAGGLTPPRVAQLKLKWAFGFP
                     GVSSARAQPSVVGGRVFVASESGDLFALDAKTGCTYWT"
BASE COUNT          405 a          800 c          795 g          331 t
ORIGIN      
        1 gaccatcagt gcgccgacga cgatgagcag ggtgatcctg cgctccacga atccggcgcc
       61 gagatagatc gtcgccactg agtacggcag ttcggtcaga gaaagcacgc cgaggtattt
      121 ccagaagctg taccgaatca gccccagcac gtagcccggt acttcggatg gcagcgccgc
      181 ctgaaacagc agcacgaggc caatcggcgc tttcaccgac acgcgatccg cgtagcgatc
      241 gagcagcggg ttcgacgtga tcgccttgac ggccggccgg ccaaggtacc ggccgatcgt
      301 gtacgcaacg acgccgccga ggatccaccc gaagagcagc agcatcgtgc tgatgccctt
      361 gccccagacg tagacgccga cgggaacgat cagcgcgctc gagacgaagg ccagcatcgc
      421 cgacgcggcc gcaaacagca cgaagaccgt gacgccgagg acggggcgat tgcggatgag
      481 ggcttccgcc tcgggcagga agccgacgag ccatccgtgc aattgcgtgg acgaggcgac
      541 gaccgccagc gcggcgacga gcaggccgag catgagcgcg cggcgcctga ccgacatgcc
      601 gccggacgcg ccgggacgct cgaccttcgc gggcgctgcc ttgcccacta gcggcgggct
      661 tcctcgagca cgctggcgtc gaagagcgac gccagcgtcg cgcgcgaccg cgggtcgcgg
      721 ccctgcttcc gggccaccca cagctcttct tcgaccatga tgtcgagcat gtcggcgggc
      781 acggcgactg gaaacgcgtg gtggggccag ctccgcgcga cgacgtcaac cggctggttg
      841 acggtcttgg caatcagcgg gaagtgaccg gccggcttcg tcttcatctc gtccaccgcg
      901 gccagcagtg cgcgcacgaa ctgcacgagc tcgcgacgcc ggcgtgaatc gttcaacacg
      961 tccgtcgtcg tgtacaggct gaacagctcg cggtagatcc ggttgtcctg gaacacgatc
     1021 gcgtccgggc cgagcgactc gagcgcgacc tgggcctcgg gctcccacat cgagatcgca
     1081 tcggccgcgc cctggacgag gccggccgcc atgccgccgc gcgggagatc gacgatcgtc
     1141 acgtcgcttt cggaaagccc cgcgctcgcc agcatgcgga cgaggctgta atgcgcggac
     1201 gtgtcgcgcg gaagcgtcac ccgcttcccg cgcaggtcgg ccagcgtccg tatgccggcc
     1261 gagcgcctgg cgacaatccg gtacagaccc tcggcaaccg tgaagagaat ccgcacattc
     1321 ggggcgccgg cgatcaggaa ctgcgtctcg gcgttcgtgg ccgcatgcgc gctgttgttc
     1381 gccaggttcc tcacgccacc gttgatgacc ctgaagccgg cgccgagcgg tccgttgtcc
     1441 gccacgtaca ccggtccgcc ttcgatggtg gacgtgctca cggccacggt gaacggcgcg
     1501 tcagccgcga gcggcaccga agcgggaacg gggttctggc tctggccacc gaggacgatc
     1561 gaggcgagga cgacgccgag ggcgagaacg atcagtcgag gcctggcttg catcatgatc
     1621 acggtccggc tgaagccgga cactacgtcc aaccacggtc cggctgaagc cggacactac
     1681 gtcagacgcg ttcgtattct cgcagactat ctataatggc gcgcggagaa agcatcacat
     1741 gaaacgacgg gccgttcttg cagcgatggt cctcctcggc gcaatcgggc tgccggccgc
     1801 ggcgcagtcg cccgacggcg ccaccgtgtt ccagaccgcg tgcgcgtcgt gtcacgccca
     1861 gccggcgccc gactcccgcg cgccgaaccg cgacacgttg gctcagctgg cgcccgaaac
     1921 gatcctcacc gcgctgacga cgggcaacat gtttcgacag ggaaccgcgc tcaccgacgc
     1981 cgagcgtcga gctgtgtctg cgttcatcgc cgggcgcccc gtgggaacgc cggccccgcc
     2041 atcgacggtg ggacgctgca cgacgactcc cccgccgctc agagccgctg atcttgtgtc
     2101 gggatggaac ggctggggca ccggcacgtc gaacacgcgt ttccagacgg cggaggcggg
     2161 cggcctgacg ccgccgaggg tggcgcagct gaagctgaag tgggcgttcg ggttccccgg
     2221 cgtcagctcc gcacgcgcgc agccgtcggt cgtcggcgga cgcgtgttcg tggcgagcga
     2281 gagcggcgac ctgttcgcgc tcgacgcgaa gaccggctgc acctactgga c
//
LOCUS       DBNGSH010000043         3219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2889404, whole genome shotgun sequence.
ACCESSION   DBNGSH010000043 DBNGSH010000000
VERSION     DBNGSH010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2889404"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..604
                     /locus_tag="AC16CY_00850"
     CDS             203..604
                     /locus_tag="AC16CY_00850"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3352350.1"
                     /translation="MTGRWLLLLVVACLVWSPSPGAHHSFAAEYLSDKPVQVSGTVSK
                     IEWLNPHVYFTVDAKGDDGRVDAWRFEGYPPNMLVRQGWKRDVTLKPGMQVTLTGWRS
                     RLDPHHAAGRQVTFADGSKLWMGPPAGTGGQ"
     gene            624..1475
                     /locus_tag="AC16CY_00855"
     CDS             624..1475
                     /locus_tag="AC16CY_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352351.1"
                     /translation="MLKAQRISIALVLAVLASALPSAQGTAKPGGLKPLGAEAQGAKA
                     AVPRTSKGKPDLSGIWVGGGFALLFGEAEMEAIRKADAAAGRRPLPPPEPPPYTPEAA
                     AKRQQYLDRRGIDDPMARCLLSGVPRITVRPLPFEIIQLPDRVVILYEVHHAFRIIPT
                     DGRPHPDDIEPSYLGDSIGRWDGDTLVVDVVGFNTNTWLAGVGTIHSEKLRVTERYTR
                     DTHDTIIYEATMEDPEVFTKPWKQYATLRLRPGERIREYECIENNEDIVRWEKLLQTD
                     PAFKKSP"
     gene            1563..2375
                     /locus_tag="AC16CY_00860"
     CDS             1563..2375
                     /locus_tag="AC16CY_00860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3352352.1"
                     /translation="MSPRKADDIRQVPTVVGPLWVDRWDQSIGKTLTRRAEYEPEWTA
                     WLTRVIQPGMRVVDIGANLGYYTVLFATRVGEHGRVLAFEPDPFNRDLLARSVRDNGL
                     EGRVTIRQVAVTDLGGSCRLHIDRSYRGLHSLSSRNRLSYDETDAIDVPAVTLDAALT
                     EFGEPDFIKIDAQGAEGAILRGASKTLGSGRRLTVMLELWPFGLANCGSSTDEVLRTL
                     EGHGFTGARVKKDRPDPIPCSWDDLSARAGRLTDDHSAINLVFRREAAGSSS"
     gene            2372..>3219
                     /locus_tag="AC16CY_00865"
     CDS             2372..>3219
                     /locus_tag="AC16CY_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR3352353.1"
                     /translation="MTRTSIALLLGAVAWAAPASRAAAPIPVMILDGESGGSYHDWQR
                     VTPALKTMLDETGLFAVTVVTAPPSGADFSAFSPAFATFQAVVMNYDVPDERWPAALK
                     ASFERYISSGGGLVAVHAADNAFPGWPAFNEMTGVGGWRGRTEQSGPYWHYRDGALVS
                     DATPGRAGSHGRRTPFPVTLRDATHPITKGLPKIWMHQGDELYARLRGPGKSMTVLAT
                     AYSDPANAGSGRDEPQLMVLSYGKGRVFHTTWGHDLTALSSVDFVVTLQRGTEWAATG
                     TVTQKVP"
BASE COUNT          569 a         1046 c         1122 g          482 t
ORIGIN      
        1 gcttcgataa cgccgctttc gcgctggccc tgccgtgtcc ggaatgcgag tgcgaagagg
       61 aagaagacgg acaaggcgga cgagggccag gggggcgcgg ccggggaaga gggggcggag
      121 ggtaggcgcc ggcccaagcc atgggttcgc agacgtgaag ccggtatact ggcgccctta
      181 caatccacgt ctggagaacc acatgacagg tcgttggctc ctcctcctcg tggtcgcgtg
      241 tctcgtgtgg tcgccctcgc cgggggcgca tcactctttt gccgccgagt acctcagcga
      301 caagcccgta caggtatccg gcaccgtctc gaaaatcgag tggctgaacc cgcacgtgta
      361 cttcaccgtg gacgcgaaag gtgacgacgg cagggtggac gcgtggcggt tcgaaggcta
      421 tcccccgaac atgctggtcc ggcaggggtg gaaacgcgac gtcacgctca agcccgggat
      481 gcaggtcacc ctcactggat ggcgctcccg cctcgatccg catcacgccg cgggccggca
      541 ggtaaccttt gccgacggca gcaagctctg gatggggccg ccagcgggga ctggcgggca
      601 gtagacacaa ggcacaaggc gcaatgctca aggctcaacg catatcgatc gcgctcgtct
      661 tggcagtcct cgcgtcggcg ctcccgagcg cgcagggcac agcgaagcca ggaggcctga
      721 agcccttggg cgccgaagcg caaggcgcga aggcggcagt tcctcgcacc tccaagggaa
      781 agccggatct gtcggggatc tgggtcggcg gcggcttcgc gctgctcttc ggcgaggcgg
      841 aaatggaagc gatccggaaa gccgacgcgg ccgcaggccg gcgcccactg ccgccgccgg
      901 agcctccgcc ctacacgccc gaagcggcgg cgaagcggca gcagtacctc gatcgccgcg
      961 gcatcgacga tccgatggcg cgctgcctgc tctcgggcgt gccgcgcatc accgtcaggc
     1021 cgctgccgtt cgagatcatt cagctccccg atcgcgtcgt catcctctac gaggtgcatc
     1081 acgcgttcag gatcattcca accgacggac gcccgcaccc ggacgacatc gagccgtcgt
     1141 acctgggcga ttcgatcggc cgctgggacg gcgacacgct cgtcgtggac gtcgtcggct
     1201 tcaacaccaa cacgtggctc gcgggggtcg gcacgattca cagcgaaaag ctccgggtga
     1261 ccgagcgcta cacccgcgac actcacgaca cgatcattta cgaagcgacg atggaggatc
     1321 ctgaagtctt cacaaagccc tggaagcaat atgcgaccct gaggctcagg ccgggagagc
     1381 ggatccgcga gtacgagtgc atcgagaaca acgaggacat cgtccgctgg gagaagctgc
     1441 tccagacgga cccggcgttc aagaagtctc cgtagagcgg ggccttcagc gtgcagtaga
     1501 gcaggggctt cagcgtgcag tagagtgggg gcttcagccc ccgcgtgcag tactctctac
     1561 aaatgtcgcc acgaaaggcc gacgacatcc ggcaggtgcc gacggtcgtg ggtcccctct
     1621 gggtggatcg ctgggaccag agcatcggga agacgctgac caggcgggcc gagtacgaac
     1681 cggaatggac ggcgtggctg acgcgcgtca ttcagccggg catgcgcgtc gtcgacatcg
     1741 gcgcgaacct gggctactac acggtcctct tcgccacgcg cgtcggcgag cacggtcgag
     1801 tgctggcgtt cgagcccgat ccgttcaacc gcgacctgct cgcgcgcagc gtccgcgaca
     1861 acgggctcga gggtcgggtg accatccgac aggtggcggt gacggacctc gggggcagct
     1921 gccggcttca catcgatcgc agctaccggg ggcttcattc actcagctcc cggaatcggc
     1981 tgtcgtacga tgagaccgat gccatcgacg tgccggcggt gacgctggat gcggcactca
     2041 cggaattcgg cgaaccagat ttcatcaaga tcgacgcgca gggcgccgag ggggcgatcc
     2101 tgcgaggcgc gtcgaagacg ctcggatccg gacgccgctt gacggtaatg ctggagctgt
     2161 ggccgttcgg tctcgccaac tgtggatcct cgacggacga agtcctgcgc acgctggagg
     2221 ggcatggatt caccggcgcg cgtgtcaaga aagaccggcc cgatccgatc ccgtgctcct
     2281 gggacgatct gtcggcgcga gcgggccggc tgaccgacga tcacagcgcc atcaacctcg
     2341 tgttcaggag agaagccgca gggagctcgt catgacgcgc acatcgattg cccttctcct
     2401 cggcgccgtg gcgtgggccg cgcccgcctc gcgcgcggct gccccgatcc cggtgatgat
     2461 cctcgacggc gagagcggcg gctcgtatca cgactggcag cgggtgacgc ccgcgttgaa
     2521 gacgatgctc gacgagacgg ggcttttcgc cgtgaccgtc gtgacggcgc cgccctccgg
     2581 cgcggacttc agcgcgttca gcccggcctt cgcgacattc caggccgtcg tgatgaacta
     2641 cgacgtgccg gacgagcgat ggccggcggc gctcaaggcg tccttcgagc ggtacatcag
     2701 cagcggcggt ggactggtcg cggtgcacgc cgccgacaac gcgttcccgg gatggccggc
     2761 cttcaacgag atgacgggag tgggcggctg gcgcgggcga acggagcagt cgggtcccta
     2821 ttggcactac cgcgacggcg cgctcgtctc cgatgcgacg ccaggccggg ccggcagcca
     2881 cggccgccgc acgccgttcc ccgtcacgct gcgcgacgcg acgcatccga ttacgaaggg
     2941 gctgccgaag atctggatgc accagggcga cgagctgtac gcgcgattgc gcggcccggg
     3001 caagagcatg acggtgctcg cgacggccta ctcggatccg gccaacgccg gcagcgggcg
     3061 agacgagccg cagctgatgg tcctgtccta cggcaagggg cgcgtcttcc acacgacgtg
     3121 gggccacgat ctcaccgcgc tcagctccgt ggacttcgtg gtgacgcttc agcgcggaac
     3181 cgagtgggcg gcgacgggca cggtgacgca gaaggtgcc
//
LOCUS       DBNGSH010000044         7134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_851764, whole genome shotgun sequence.
ACCESSION   DBNGSH010000044 DBNGSH010000000
VERSION     DBNGSH010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_851764"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="AC16CY_00870"
     CDS             <1..238
                     /locus_tag="AC16CY_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3352354.1"
                     /translation="FRRGDLDEAEKYLSAAAARLPENSEIQDHLGDLFARRGRMAEAV
                     DAWTRALKGDSQDVDKATIEKKISNAKGKMQNAK"
     gene            382..1020
                     /locus_tag="AC16CY_00875"
     CDS             382..1020
                     /locus_tag="AC16CY_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015512.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIR3352355.1"
                     /translation="MRTLTAELALRGRAGRQRLRGRLHAGFERPDSMRLELVAPFGPP
                     VFILTSRGTTAVLLLTREDRVVRGESAEAILGALTGVSLSPADLQAILDGCVVPEPRA
                     TGGRQHADGSASIDLAGGARLYLRRADTWQVRAARRAGWQVEYPAWEGSFPASVRLQS
                     DSPSVQVDVTATLTQIETNAGIRPDAFSIDVPSSARPLSIAELRDAGPLGDK"
     gene            1020..1982
                     /locus_tag="AC16CY_00880"
     CDS             1020..1982
                     /locus_tag="AC16CY_00880"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3352356.1"
                     /translation="MPGPRSGTASVKAITVRAHAKINLDLRVLGMRPDGFHELRTVFQ
                     AVALHDSVECIPRRGPLAIECEMAGVPLDRSNLAWRAAEALWRALRRDGEVRDILIRL
                     HKRIPLQGGLGGGSTDAAATLLALCRAWRVPVRANQLVDVASVLGADVPFFLSGGTAL
                     GLGRGDEIYPLADLPRHWVVLLVPGFGVATADAYEWYDAERDPTRGPMVREAQYVPGP
                     WPSRAAQMINDLEAPIARHHPEIEQMKTALRRAGALAAAMSGSGATVFGLFQKRSEAA
                     SAVARLSASGWGVILTESLSRGEYARRSQPVQVNLARRRNSRLH"
     gene            2090..2164
                     /locus_tag="AC16CY_00885"
     tRNA            2090..2164
                     /locus_tag="AC16CY_00885"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2122..2124,aa:Gln,seq:ttg)
     gene            2197..3177
                     /locus_tag="AC16CY_00890"
     CDS             2197..3177
                     /locus_tag="AC16CY_00890"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR3352357.1"
                     /translation="MRRPLSGDGLVGTGFGELKVFSGNAHRQLAQEIADCLGIPLGQA
                     RLRRFPDSEVSFQIDENIRGADVFIIQPTSNPVDQHLMELLVMIDAFRRSSAARITAV
                     IPYYGYARQDRKDKPRVPISAKLVANLLSAAGTNRVLTMDLHKAQIQGFFDIPVDHLF
                     AAPVIIEYCSKLQSSNLTIVSPDAGGAERARAYAKRLDAELAIVDKRRSEDGTAEVMN
                     VIGDVQGRTCILQDDIIDTAGTIQKASIALKDAGAERVMACAVHGVLSGPAIQRIEDS
                     PLDQLIVTNSVPLRGEAERCGKIRVLTVARLLGQAIRNIHEETSVSSLFI"
     gene            3216..3920
                     /locus_tag="AC16CY_00895"
     CDS             3216..3920
                     /locus_tag="AC16CY_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR3352358.1"
                     /translation="MDAVLEAVKREGRGKNEANRLRASGQVPAVVYGTGSKGKAPEGV
                     AVSVDPKAVLRILHSDSGANTLITLKLDGRQSQVMVKEYQLDPLTHKLLHADFYQLAM
                     DKAIIVSVPIQVKGEPKGVKDQGGLLDFVTRDVEIECLPRDIPENIVIDVSELMLHQS
                     IRMRDVAPDPKWKPMTDGDTMLVHVVMPKAEEAAVAAETAEAAPAAAAEPEVIKKGKT
                     EEKEEKEAEKADKAKK"
     gene            3950..4540
                     /gene="pth"
                     /locus_tag="AC16CY_00900"
     CDS             3950..4540
                     /gene="pth"
                     /locus_tag="AC16CY_00900"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR3352359.1"
                     /translation="MKLIAGLGNPGREYRETRHNVGFMVVDEIARRHGLTWAQAPSQI
                     PDAFVAKRFSPTPVLLAKPLTYMNLSGDVVAGLSRYYDVPAGDVLVVVDEAALPFGRL
                     RARARGSAGGHNGLKSIIERLGTTEFPRLRLGVGRGDGRRDLADHVLSTFERDELAEL
                     ESIITRAADAAEMFAVEGIVKVMNVYNPDDTAPETD"
     gene            4724..5176
                     /gene="rpsF"
                     /locus_tag="AC16CY_00905"
     CDS             4724..5176
                     /gene="rpsF"
                     /locus_tag="AC16CY_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR3352360.1"
                     /translation="MSRKYELVYVASPEATDEQVADLHTQVEAIVQRIGGQIEKTDNW
                     GRRKLAYEIGRHKEGTYVLEVILGGGDLMKEIDRRLKVTDLVIRHLIVRVDEDQGVVE
                     RTRAHRTEGSRRRRVARGLPPERQPGEGQRGELDDDRDDRFDMGEDLR"
     gene            5173..5469
                     /gene="rpsR"
                     /locus_tag="AC16CY_00910"
     CDS             5173..5469
                     /gene="rpsR"
                     /locus_tag="AC16CY_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR3352361.1"
                     /translation="MNGRKSFGGGGRGKTAAGQQGKDGQRRPMFRRRKVCKFCADKID
                     DINYKDVRMLAPFVPERAKVLPRRISGTCAIHQRKLRTAIMRARQLALIPYTSE"
     gene            5534..5989
                     /gene="rplI"
                     /locus_tag="AC16CY_00915"
     CDS             5534..5989
                     /gene="rplI"
                     /locus_tag="AC16CY_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3352362.1"
                     /translation="MEVILREHVDNLGRRGEIVKVAAGYARNYLLPRKLALPATEGNK
                     KHVERERRIMEAKEAQEKGQAEAVAARLALLEVSIARRVGETDQLYGSVTASDIAEYL
                     KGKGFEIDRRKLILPEPIKTLGDHKVPLKLHREVTVPLTVKVTKEGTEG"
     gene            6028..6498
                     /locus_tag="AC16CY_00920"
     CDS             6028..6498
                     /locus_tag="AC16CY_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352363.1"
                     /translation="MALVAIPTAVVSISWMLGITRDSSVPNVFDLAILFIFMLPVPAA
                     IAVFALLAPLAITTDHVLRGRTTRLINVVLGAALGVAAYTLYFAGSALFRLPTPFTPR
                     RMIADGASLDTLYRILTAPQTPFMLAVTVVVLTSFVVAGMLVGLGLRRPRSVTG"
     gene            complement(6893..>7134)
                     /locus_tag="AC16CY_00925"
     CDS             complement(6893..>7134)
                     /locus_tag="AC16CY_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HIR3352364.1"
                     /translation="AAKTLQVHLGWVVQTYNATEALANLIHPFWMLPLVGILGLKARD
                     IVGYSMLQFVVHVPLVLFLVWILNYTLEYVAPVLP"
BASE COUNT         1279 a         2223 c         2455 g         1177 t
ORIGIN      
        1 cttccgccgc ggcgacctcg acgaggcgga gaagtatctg tcggccgccg ccgcccggct
       61 tcccgagaac tcagagatcc aggaccacct cggcgatctg ttcgcccgcc gggggcgcat
      121 ggccgaagcc gttgacgcct ggacgcgcgc gctcaagggc gacagtcagg acgtcgacaa
      181 ggcgacgatc gagaagaaga tcagtaacgc aaaaggcaag atgcaaaatg caaaatgaag
      241 cacctgcatc ttgcattgtg cattgcgtgt gtcgcttccg cttcgtgcgc accgaaaaga
      301 ttcaccgtcc cgactgatcc gggctcgccc cttccgaaat acgaggagat ccacgcgcag
      361 gtcgctgcga cctgccgcgg cgtgcgcacg ctgaccgccg agctcgcttt gcgcggccgc
      421 gccggccgcc agcgtcttcg cgggcggctg catgccggat tcgagcggcc ggactcgatg
      481 cggctcgagt tggtcgcccc gttcgggccg cccgtcttca tcctgacgtc gcgcggaacg
      541 actgccgtcc ttctactgac tcgcgaggac cgcgtcgtcc ggggcgagtc ggccgaggcc
      601 attctcgggg cgctgaccgg tgtatcgctg tcgcccgccg atctgcaggc cattctcgac
      661 ggctgcgtcg tgcccgaacc acgggcgacg ggcggccgcc agcacgccga tggatcggca
      721 tcgatcgacc tcgccggcgg cgcgaggctc tacctccgcc gcgccgatac gtggcaggtt
      781 cgcgcagcac gccgcgccgg gtggcaggtc gagtaccccg cgtgggaggg ctcgtttccc
      841 gcgtcggttc gactgcagtc cgattcccct tcggttcagg tggacgtgac cgcgactctc
      901 acgcaaatcg aaacgaacgc gggcatcagg ccggacgcgt tttcgatcga cgttccctcg
      961 agcgcgcgtc cgctgtcgat cgcggagctc cgcgacgctg gaccgctcgg agacaagtga
     1021 tgcctggtcc acgctccggg acggcgtccg tgaaggcgat caccgtgcgc gctcacgcaa
     1081 aaatcaatct ggatctgcgc gtgctcggca tgaggccgga cggttttcac gagctccgga
     1141 cggtcttcca ggcggtggcg ctgcacgata gcgtcgagtg catcccgcgg cgggggccgc
     1201 tggcgatcga gtgcgaaatg gccggtgttc ctctcgaccg ttcgaacctc gcgtggcggg
     1261 cggcggaggc gctgtggcgc gcgctcaggc gcgacgggga ggtcagggac atcctcatcc
     1321 ggctacacaa gcggattccc ctgcagggag ggctgggcgg cggcagcacc gacgccgctg
     1381 caacgctgct ggcactgtgc cgggcgtggc gtgtgccggt acgtgccaac cagctcgttg
     1441 acgtggcctc cgtacttggc gccgacgtgc cgttctttct gtcgggcggg accgctctcg
     1501 gcctcgggcg cggcgacgag atctaccctc tcgccgattt gccgcgccac tgggtcgtgc
     1561 tgctcgtgcc cggcttcggt gtggccaccg cggacgccta cgagtggtac gacgccgagc
     1621 gagaccccac gcgcgggccg atggtgcggg aggcgcagta cgtgcccggg ccctggccct
     1681 cgcgcgcggc ccagatgatc aacgacctgg aagcgcccat cgcgcggcat catcccgaga
     1741 tcgagcagat gaagaccgcc cttcgccggg ctggcgcgct ggcggcggcg atgtcgggca
     1801 gcggcgcgac cgtgttcggg ttgtttcaga agcggtcgga ggcggcctcc gccgtcgccc
     1861 gtctgtccgc gtcgggctgg ggcgtgatcc tgaccgagtc gttgagccgc ggcgagtacg
     1921 cgcggagaag ccagccggtg caggtgaatc ttgcccggcg ccgcaactct cgactacact
     1981 aagggattgc acccctccgt tcggtgactc ctgtagatct cacgcgaggc aaagcgggtc
     2041 cggcaggagg atctgcagct tgaggtcccg gcgggccgtg gtgtcgtagt ggggcgtggc
     2101 caagcggtaa ggcgcgggac tttggatccc gtattcggag gttcgaatcc tcccgcccca
     2161 accagctcgg agatggtgcg ccaggcgcag tgaatgatga gacgacctct cagcggtgac
     2221 ggattggtag ggaccggatt cggtgagctg aaggtgtttt cgggcaacgc ccaccggcag
     2281 ttggcccagg agatcgccga ctgcctcggc attccgctcg ggcaggcgcg gctcaggcga
     2341 ttcccggaca gcgaggtctc gtttcagatc gacgagaaca ttcgcggcgc cgacgtcttc
     2401 atcatccagc cgacctcgaa tccggtggat cagcacctga tggagctgct ggtgatgatc
     2461 gatgcgttcc ggcggtcctc cgccgcgcgc atcaccgcgg tcattcccta ctacggctac
     2521 gcgcgccagg accggaagga caagccgcgc gtgccgatct cggccaagct ggtggcgaac
     2581 ctgctgagcg cggcgggcac caaccgcgtg ctgacgatgg atctgcacaa ggcgcagatt
     2641 cagggattct tcgacatccc cgtcgaccac ctgtttgccg cgccggtgat catcgagtac
     2701 tgctcgaagc tgcagtcgag caacctgacg atcgtgtcgc cggatgcggg cggggcggag
     2761 cgggcacgag cctacgccaa acgcctcgac gcggagctgg ccattgtcga caaacggcgc
     2821 agcgaggatg gcactgccga ggtcatgaac gtgatcggcg acgtgcaggg gcgcacctgc
     2881 attctgcaag acgacatcat cgacaccgcc ggcacgattc agaaagcctc gatcgcgctc
     2941 aaggacgcgg gcgccgaacg cgtcatggcg tgcgccgtgc acggcgtgct gtcgggaccg
     3001 gcgatccagc gcatcgagga ttcgccgctc gaccagttga tcgtgaccaa ctcggtcccg
     3061 ctgcgcggcg aggccgagcg atgcggcaag atcagggtgt tgaccgttgc ccggctgctc
     3121 ggccaggcga tacggaacat ccacgaagag acgtcggttt cttcgctctt tatctgaccg
     3181 tggaaccgta gaccgtagac gacagagatc aggttatgga cgcagtacta gaagcagtca
     3241 agagagaagg gcgagggaag aacgaggcga accggttgcg ggcctcgggg caagtcccgg
     3301 ccgtggtgta tggaaccgga tcgaagggca aggcgcccga gggcgtcgcg gtgtcggttg
     3361 acccgaaggc cgtgctgcgg atcctccact cagactcggg cgcgaacacg ctgatcactc
     3421 tgaagctgga tggcaggcag tcgcaggtga tggtcaagga gtaccagctc gacccgctga
     3481 cccacaagct gctccacgcc gacttctatc agctggcgat ggacaaggcc atcattgtca
     3541 gcgtgcccat ccaggtcaag ggtgagccga agggcgtgaa ggatcagggc ggcctcctgg
     3601 acttcgtgac gcgcgacgtc gagatcgagt gtctgccgag agacatcccg gagaacatcg
     3661 tcatcgacgt gagcgagttg atgctgcacc agtcgatccg catgcgcgac gtggctccgg
     3721 atccaaaatg gaagccgatg accgacggcg acacgatgct cgtccacgtc gtgatgccga
     3781 aggccgagga ggccgcggtg gcggccgaga cggccgaagc ggcgccggcg gcggcggccg
     3841 agcccgaggt catcaagaag ggcaagacgg aggagaagga agagaaagag gccgagaagg
     3901 cggacaaggc gaagaagtag aggcgggcct tcacacccgc ctgcacgccg tgaaactcat
     3961 tgcggggcta gggaatccgg ggcgcgagta tcgcgagacc cggcacaacg tcggcttcat
     4021 ggtcgtcgac gagatcgcgc ggcgccatgg gctcacctgg gcgcaggcgc cttctcagat
     4081 ccccgatgcg ttcgtcgcga agcggttttc gccgacaccc gtgctgctcg ccaagccgct
     4141 cacgtacatg aacctgagtg gcgatgtcgt cgccggactg tcgaggtatt acgatgttcc
     4201 ggccggcgat gtgctcgtcg tcgtggacga agccgccctg ccgttcgggc ggctgcgggc
     4261 acgggcccgc gggtcggcgg gcgggcacaa cggtctcaag tcgatcatcg aacggcttgg
     4321 gacgacggaa tttccccggc tgcggctggg ggtggggcgc ggagacggca ggagggatct
     4381 tgccgaccac gtcctgtcga cgttcgagcg cgacgaactc gccgagctcg aatcgatcat
     4441 cacccgggcg gctgatgccg ccgagatgtt cgccgtggag ggcatcgtca aggtgatgaa
     4501 cgtgtacaac ccggacgata cggcgccgga aacagactga ctccttgctg ccgcgcgaag
     4561 gccggcagcc gtgagcttcg gcggggtagt gactgaccga aactaaccac ggagtcacgg
     4621 agtcacggag acttggtatt ttcctccgtg tctctgtgtc tccgtggttt gtgggcagtg
     4681 aatgctccag gctgaagcgg gtcaacgtcc agaaggagac gtaatgagca ggaagtacga
     4741 actcgtgtat gtcgcgtcgc ccgaggcgac cgacgaacag gtggcggacc tgcacacgca
     4801 ggtcgaggcc atcgtccaac ggatcggcgg ccagatcgag aagaccgaca actggggccg
     4861 ccggaagctt gcctacgaga tcggccgcca caaggaaggc acctacgtgc tggaggtgat
     4921 cctgggcggc ggggatctga tgaaggagat cgaccggcga ctgaaggtca ccgatctcgt
     4981 catccgtcac ctcatcgtca gggtagacga ggatcagggc gtggtcgagc gcacgcgcgc
     5041 gcatcgtacc gaaggaagcc gtcgccggcg cgtcgcgcgg ggactcccgc cggagcgcca
     5101 gccgggtgaa ggccagcgcg gcgagctcga cgacgaccgt gacgatcgct tcgacatggg
     5161 ggaggacctc cgatgaacgg cagaaaaagc ttcggcggtg gcggccgcgg gaagacggcc
     5221 gccggtcagc aggggaagga cggccagcgg cgtccgatgt tccgtcgccg caaggtctgc
     5281 aagttctgcg cggacaagat cgacgacatc aactacaagg acgtccggat gctggcgccg
     5341 ttcgtgcccg aacgcgcgaa ggtgcttcct cgtcgcatct ccggcacctg cgcgattcac
     5401 cagcggaagc tgcgaacggc gatcatgcgg gcgcgccagc tggcgctgat tccgtacacg
     5461 tccgagtagc aggcagtagg cggtacgcag attcgtagcg cggtggcttc agccaccgcg
     5521 ttgaggacac gtaatggaag tcattctgag agaacacgtg gacaacctcg gccgccgggg
     5581 cgaaatcgtc aaagtcgccg ccggctacgc gaggaactac ctcctgcctc gcaagctcgc
     5641 gcttcccgcc accgaaggca acaagaagca cgtcgagcgc gagcgcagga tcatggaagc
     5701 gaaagaggcc caggagaagg gacaggccga agcggtcgcc gcgcggctcg ccctcctgga
     5761 ggtgtcgatc gcgcgccgcg tcggcgagac cgatcagttg tacgggtccg tgacggcttc
     5821 cgacatcgcc gagtacctga agggcaaggg cttcgagatc gatcgccgaa agctgatcct
     5881 gccggagccg atcaagacgc tcggcgacca caaggtcccg ctcaagctgc accgcgaagt
     5941 caccgtgccg ctgacggtga aggtcacgaa ggaagggacg gaaggctgac gcgtcacagg
     6001 atttgggcct tttcgttcct ggcatgggtg gcgctggtcg ccatcccaac ggccgtcgtg
     6061 tcgatcagtt ggatgctcgg catcacacgg gattcttccg tgccgaacgt cttcgacttg
     6121 gcgatccttt tcattttcat gttgccggtg cccgccgcca ttgcggtgtt cgcgcttctc
     6181 gcgccgctcg caatcacaac cgatcatgtc ttgcgcggtc ggacgacgcg cttgataaac
     6241 gtcgtcctcg gagccgcgct tggcgtggct gcatacacgc tctatttcgc aggctccgcg
     6301 ttatttcggc ttccgacgcc gttcacccct cgccgcatga tcgctgatgg agcgtccctc
     6361 gacacgctgt atcgcatcct cacggcgccg caaaccccat tcatgttggc ggtgactgta
     6421 gttgtcttga cgagctttgt cgtggccggg atgctcgtgg gactggggtt gcggcggccg
     6481 cgcagtgtta caggttgacg ggcgaggctg agaggcgatc cgatcgggac cggctctttt
     6541 gggctctcga cctggaacgg tctgttactt gcggcgagcg caggcgcgtt ttccgatgcg
     6601 ctcacacgat gcgcttttcg attgaccacg aactcccgac gatggcatcg tcgtcagccg
     6661 tgagcgaacg ggctgagccg gttgaaagtg gcgccggaag ctgcatgacg acgtcggcgg
     6721 gtcgctgaag accgcgggtt ctgtatcaac gactcgagca gcgggccggc atctgaaggc
     6781 tagactggcc caggcgggtc tgaagaccgc ctctacacaa cataagccct gaatccttag
     6841 acagccctga ggccttagac agccctgagg cctggggcct gagccctggg gcctacggaa
     6901 gcacgggcgc aacgtactcg agcgtgtagt tcaagatcca cacgaggaac agcaccagcg
     6961 gcacgtggac gacgaactgc agcatcgagt agcccacgat gtcgcgcgcc ttcaggccca
     7021 ggatgccgac gagcggcagc atccagaacg ggtggatcag gttcgccagc gcctccgtgg
     7081 cgttgtaggt ctggaccacc cacccgaggt ggacctgcag cgtcttggcc gcct
//
LOCUS       DBNGSH010000045         2856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2210103, whole genome shotgun sequence.
ACCESSION   DBNGSH010000045 DBNGSH010000000
VERSION     DBNGSH010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2210103"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2445
                     /locus_tag="AC16CY_00930"
     CDS             <1..2445
                     /locus_tag="AC16CY_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3352365.1"
                     /translation="SGRHHYLIARPDQKERVLDTYVKMVTAQPPPRPGRGGPRPAAGL
                     GGAAITLLGAALAGLVLAQSARISGQADEFDSAVRPVLTGTCAACHNAQLASGGMSVA
                     ELTSPESLASRRDAWEKILQRLRAGDMPPAGVARPAPAQLAAMTGYIERAFERADAAE
                     KPDPGRLTAHRLNRNEYTNTIRDLLGVRFRAEKYFPADDSGDGFDNMADVLTVSPLLM
                     ERYLAAAERIAAWAISTEIPGKPIEADYRARDRKIRRIDRSTIEAEHRVEFAGEYVVR
                     FGLPGERPPVDGRDAAPVTLGFWMNGAELATKVVQTKPSGLVYFDPYSEEEIRLHLPE
                     GDHVFRAGFIGDDFVKTLPNDAAYDRRKNKFLDSIVFVGPFPSTVEKESRKKILTCDP
                     ASGRACIERIVTDLARRAYRRPPTRGEVDRLLRFVDVARKDGGSAERGVQLAVQALLV
                     SPNFLFRIERDPDPRNPALVHEVSPFELASRVSYFLWSSMPDDELLALAGSGRLRDAK
                     VLEAQVDRMLADPRASAFAENFAGQWLETRNLDVVTPDPDAFKEWNPELREAMKLETT
                     MFFEHVLRENRPISDFLNADYTFLNERLAAHYGIAGVTGPAFRRVALTTDRRGGVLSQ
                     AGVLTVSSYPTRTSAVIRGRYVLQNILGMPPAPPPPDVPLLSESAGDGTRSMREQLEV
                     HRTNPTCAACHRNMDALGFGLENYDAIGRWRDLDGKFPVDASGTLPDGRRFQTAGEMR
                     ALLVSQLPQFSRTLTSKMMTYALRRGLEPYDRRTVESVSRAVAADGYRLRTMVHQIVR
                     SLPFQARRGEDVKGSR"
     gene            2445..>2856
                     /locus_tag="AC16CY_00935"
     CDS             2445..>2856
                     /locus_tag="AC16CY_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3352366.1"
                     /translation="MFITRQALPRRTFLRGIGATVALPFLDAMTPALARAQSRKAPVR
                     MAFVYVPNGIDMRHWNPSYEGPLAELPQTLAPLTPLKNDILLLGNLTHNTGRALLDGP
                     GDHGRCCGSYLTGVQVKKTVTDIKASVSCDQLVAN"
BASE COUNT          442 a         1025 c          963 g          426 t
ORIGIN      
        1 tcgggccggc atcactatct gatcgcgagg ccggatcaga aggagcgcgt gctcgacacc
       61 tacgtgaaga tggtgacggc gcagcccccg ccccgccccg ggcgcggcgg gccgaggccc
      121 gcggcaggcc ttggcggcgc cgcaatcacg cttcttggcg ctgctctggc cggcctcgtg
      181 ctggcacagt cggcgcgcat cagcggacag gccgacgagt tcgacagcgc ggtgcggccc
      241 gttctcacgg gaacgtgcgc cgcctgtcac aacgcgcagc tcgcctctgg cgggatgagc
      301 gtcgcggagc tcacctcacc ggagtcgctc gcgagtcggc gcgacgcgtg ggagaagatc
      361 ctgcagcgcc ttcgcgcggg cgacatgccc ccggccggcg tcgcgcggcc cgccccggcg
      421 cagcttgccg cgatgacggg ctacatcgag cgcgccttcg agcgcgccga cgcggccgag
      481 aagcccgatc ccggccgcct gacggctcat cgcctgaacc gcaacgaata caccaacacg
      541 atccgggatc tgctcggcgt ccgcttccgc gcggagaagt acttccccgc ggacgactcg
      601 ggcgacgggt tcgacaacat ggcggacgtg ctcacggtct cgccgctcct gatggagcgg
      661 tatctcgccg ccgccgagcg catcgccgcg tgggcgatct ccaccgagat cccgggaaag
      721 ccgatcgagg ccgattaccg cgcgcgcgac cgcaagatcc ggcgcatcga ccgcagcacg
      781 atcgaggccg agcaccgcgt cgagttcgcc ggcgaatacg tcgtccgctt cggcctgccc
      841 ggcgagcgcc cgccggttga cggccgcgac gccgcgccgg tgacgctcgg cttctggatg
      901 aacggcgccg agctcgccac caaggtcgtc cagacgaagc cgtcaggcct cgtctacttc
      961 gatccgtact ccgaggaaga gatcaggctg cacttgccgg aaggcgatca cgtcttccgc
     1021 gccgggttca tcggcgacga cttcgtcaag acccttccaa acgacgccgc ctacgaccgc
     1081 cggaagaaca agttcctgga ctcgatcgtc ttcgtcggcc cgtttccgtc cacggttgag
     1141 aaagagagcc ggaagaagat cctcacctgc gatcccgcgt cgggccgcgc gtgcatcgag
     1201 cggatcgtga ccgaccttgc gcgccgcgcg taccgacggc cgcccacgcg cggcgaggtg
     1261 gatcgcctgc tgcgcttcgt ggacgtggca cggaaggatg gcgggtcggc ggagcggggc
     1321 gtgcagctgg cagttcaggc gctgctcgtg tcgcccaact tcctgttccg catcgagcgc
     1381 gaccccgacc cgcgcaaccc cgcgctcgtg cacgaggtct cgcccttcga gctcgcttcg
     1441 cgcgtcagct acttcctctg gagctcgatg ccggacgacg aactgctcgc gctcgccggg
     1501 tcgggccggc ttcgcgacgc gaaggtgctc gaggcgcagg tcgatcggat gctcgccgat
     1561 ccgcgcgcgt ctgccttcgc ggagaacttc gcggggcagt ggctcgagac gcgcaacctc
     1621 gacgtcgtca cgcccgatcc cgacgcgttc aaagaatgga acccggagct gcgcgaggcg
     1681 atgaagctcg aaacgacgat gttcttcgag cacgtgctgc gggaaaaccg gccgatcagc
     1741 gatttcctga atgcggacta tacgttcctc aacgagcgtc tcgcggcgca ttacggcatc
     1801 gccggcgtga ccggtcccgc gttccgccgc gtggcgctca cgaccgatcg ccggggcggt
     1861 gtcctgagcc aggccggcgt gctgacggtg tcgagctatc ccacgcgcac ctcggccgtg
     1921 attcgcggcc ggtatgtcct gcagaacatc ctcgggatgc ccccggcgcc gccgccgccg
     1981 gacgtgccgc tgctgagcga atcggccggc gacgggacgc gctcgatgcg cgaacagctc
     2041 gaggtgcacc gcacgaaccc gacgtgcgcc gcctgtcacc gcaacatgga cgcgctcggc
     2101 ttcggcctgg agaactacga cgccatcggc cgctggcgcg acctggacgg caagtttccc
     2161 gtggacgcca gcggcacgct tcccgacggc cggcggttcc agacggcggg tgaaatgcgg
     2221 gcgcttctgg tctcgcaatt gccgcagttc tcgcgtaccc tgacctcgaa gatgatgacc
     2281 tatgccctcc ggcgcgggct cgagccgtac gaccggcgga cggtggagtc ggtcagccgc
     2341 gcggtcgcgg ccgacggcta ccggctgcga acgatggtcc atcagatcgt caggagcctg
     2401 ccgttccagg cgcggcgcgg cgaggacgtg aaagggtcgc ggtgatgttc atcacgcgtc
     2461 aggcccttcc tcggcgtacc ttcctgcgcg gcatcggcgc gacggtcgcg ctgcccttcc
     2521 tggacgcgat gacgcccgcg ctcgcccgcg cgcagtcgcg gaaggcgccg gtccgcatgg
     2581 cgttcgtcta cgtgccgaac ggcatcgaca tgcggcactg gaacccgtcg tacgagggac
     2641 cgctcgcgga gctgccgcag acgctcgcgc cgctcacgcc tctgaagaac gacatcctcc
     2701 tgctcggcaa cctcacgcac aacacgggac gcgcgctcct cgacggtccc ggcgaccacg
     2761 gccgctgctg cgggtcgtac ctcaccggcg tgcaggtgaa gaagaccgtg accgacatca
     2821 aggcgagcgt ctcctgcgac cagctcgtcg cgaatc
//
LOCUS       DBNGSH010000046         2193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2040574, whole genome shotgun sequence.
ACCESSION   DBNGSH010000046 DBNGSH010000000
VERSION     DBNGSH010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2040574"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..575
                     /locus_tag="AC16CY_00940"
     CDS             102..575
                     /locus_tag="AC16CY_00940"
                     /inference="COORDINATES: protein motif:HMM:NF042760.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR3352367.1"
                     /translation="MATDAVYESSLAWDPERPHTTLITCVDGRWYHHFQEFARVHLGA
                     GERTDFLAVPGGIEPLTLFDLLPKDFNFFRRRLEGLVEAHGTKRIVAIAHQDCAWYRA
                     RRIGPITIDLRDRQLADLRRSARRLREMFDNIAVETYFARLSATQPAKVVFDLIQ"
     gene            578..>2193
                     /locus_tag="AC16CY_00945"
     CDS             578..>2193
                     /locus_tag="AC16CY_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3352368.1"
                     /translation="MRTPAAVVCAAFLAVMVPSAQRPAFTPVTEEMLLKPGPDDWLMY
                     SRTYDAQRFSPLDQITTKNVGQLREVFKKELPAGPHESIPIVYRGVMYLQLPGNALQA
                     LDAATGAVIWEHKRPASASRAKAIAIYEDMVYSTTPDGFIVALDARTGEVRWETKSTG
                     RLTAGAMVVEGKVLSGRACAPRREDCYIAAHDAKTGKEVWRFYTAAGSTEPGGDTWAG
                     APDETRAASTWALPGGYDPVRRLIYWGVANPTPNTRANRHGGNSNAIPTESPADLYSN
                     STVALDPDTGKLRWYYQHLPGDDWDEDYPNERTLLRTSFNPDPTFVKWINPDVKRGEQ
                     RDVAVMVGEGGGIFVIDRGTGQFLWANPFPFDTPDFLISNVDGKTGRVSLNKSKLFTG
                     PSDRRVICFWNTRSYWPTAYHPGVNALFVPYVENCLDMTTAGPDGNPRERRVGIPRPG
                     STPDTWAGLMKIDMSTGVMKPIHTGRAPGNGAVLTTAGNLVFWGDLDQKFRAFDAGSG
                     TLLWEQPLGGPIQNSTITYAVGGKQYVAVLT"
BASE COUNT          397 a          754 c          716 g          326 t
ORIGIN      
        1 gatgctgaag gatcagttcg ggatcgacgt cgcccggctg atgcaggcca agacggaggc
       61 cgccgccgac gaggcggaac gcaagaccgg aaaacagtga gatggccacc gatgccgtct
      121 acgagagctc tctcgcgtgg gatcccgagc ggccgcacac cacgctcatc acgtgcgtcg
      181 acggccgctg gtaccatcac tttcaggagt tcgcgcgcgt ccacctgggt gcgggcgagc
      241 ggacggactt cctggccgtg cccggaggca tcgagccctt gacgctcttc gacctcctgc
      301 cgaaggactt caacttcttc cggcgacgcc tcgaaggcct cgtcgaggcg cacgggacga
      361 agcggatcgt cgccatcgca caccaggact gcgcctggta ccgggcgcgt cgaatcggac
      421 ccatcacgat tgacctgcga gacaggcagc tcgccgacct ccggcgttcg gcgcgccggc
      481 tgcgtgagat gttcgacaac atcgccgttg agacctactt cgcgcggctg agcgcgacgc
      541 aacccgcgaa ggtcgtcttc gatctcattc aatgaccatg cgcactccag ctgccgttgt
      601 ctgtgccgcc ttcctggccg tcatggtgcc cagtgcccag cggccggcct tcacgcccgt
      661 gaccgaggag atgctcctca agcccggccc ggacgactgg ctgatgtaca gccggaccta
      721 cgacgcacag cggttcagcc cgctcgacca gatcacgacg aagaacgtcg gtcagctccg
      781 cgaggtgttc aagaaggagc ttcccgcggg gccgcacgag agcattccga tcgtctatcg
      841 cggcgtgatg tatctgcagc tgcccggcaa cgccctgcag gcgctggacg cggcgaccgg
      901 cgccgtgatc tgggagcaca agaggcccgc gagcgcgtcg cgagccaagg cgatcgcgat
      961 ctacgaggac atggtgtaca gcacgacgcc cgacggcttc atcgtggcgc tcgacgcacg
     1021 aacgggcgag gtccggtggg aaaccaagtc cacgggccgg ttgaccgcgg gcgcgatggt
     1081 cgtggaaggg aaggtgctga gcggccgcgc gtgcgcaccc cgccgcgagg actgctacat
     1141 cgccgcgcac gacgccaaga cgggcaagga ggtctggcgc ttctacacgg ctgcaggctc
     1201 caccgagccc ggcggcgaca cgtgggccgg cgcgcccgac gagacgcgtg ccgcttccac
     1261 gtgggccctg cccggcggct acgacccggt tcgccgcctc atctactggg gggtggccaa
     1321 ccccacgccg aacacgcgcg cgaaccggca cggaggcaac agcaacgcga ttcccaccga
     1381 gtcgccggcg gatctgtaca gcaactccac cgtggcgctc gacccggaca cgggcaagct
     1441 gcgctggtac taccagcacc ttcccggcga cgactgggac gaggactacc cgaacgagcg
     1501 cacgctgctg cgaacctcgt tcaacccgga cccgacgttc gtgaaatgga tcaaccccga
     1561 cgtcaagcgc ggggagcagc gtgacgtggc cgtcatggtg ggcgagggtg gcggcatctt
     1621 cgtgatcgac cgcggcacgg gccagttcct ctgggcgaac ccgttcccgt tcgacacgcc
     1681 agacttcctg atctcgaacg tcgacggcaa gacgggacgc gtgtcgctca acaagagcaa
     1741 gctgttcacc ggtccctccg atcgccgagt catctgcttc tggaacacgc gcagctattg
     1801 gcccaccgcg tatcaccccg gtgtcaacgc gctgttcgtg ccgtacgtcg agaactgcct
     1861 tgacatgacg accgcgggac cggacggcaa tccgcgtgaa cgccgcgtcg gcattccgcg
     1921 acctggatcc actccggaca catgggcggg tctcatgaag atcgacatgt ccaccggcgt
     1981 gatgaagccg atccacacgg ggcgggctcc tggcaacggc gccgtgctga cgacggccgg
     2041 caacctcgtg ttctggggag atctcgatca gaaattccgc gcgttcgacg ccggcagcgg
     2101 cacgctgctc tgggagcagc cgctcggggg ccccatccag aacagcacga ttacctacgc
     2161 ggtcggcggc aagcagtacg tcgcggtcct cac
//
LOCUS       DBNGSH010000047         7427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2889566, whole genome shotgun sequence.
ACCESSION   DBNGSH010000047 DBNGSH010000000
VERSION     DBNGSH010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2889566"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..982)
                     /locus_tag="AC16CY_00950"
     CDS             complement(44..982)
                     /locus_tag="AC16CY_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3352369.1"
                     /translation="MSEPAVVFDEVSKFYGEVLGVNRVTLNIPPGITSLVGPNGSGKT
                     TLMNLMTGLIFPDHGSILMRGISPRDPETLMRITGYATQYDSAPRWATGFTFITTGLL
                     LFGYGRSDAEERAWKALQRLGLTDAANRKVAGYSKGMRQRVRLAQAIAHDPDLLVLDE
                     PLNGLDPLVRAETIDLFRSLASEGKHVIISSHVLQEVDLISDQVVLIANGMIVAEGQI
                     RKVRDEIQEHPSQFIVRCRGRDASGVAALLFSEDHITEIKLNDDRLGMLVMTRDREQF
                     ARALGRIALDGHRIESVVPADENVDALYEYLIGGQS"
     gene            complement(979..1839)
                     /locus_tag="AC16CY_00955"
     CDS             complement(979..1839)
                     /locus_tag="AC16CY_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352370.1"
                     /translation="MAVYKRGYRRYAGTLTGRWTRFMVLPRYAWKELYQQRLVLLLTT
                     LAFVWPLLCAGFIYLTNHVQLLQGLEPEFREFIQVDGRFFSIFMYVQGACAVFLTALA
                     GPGLLAPDLANNALPLYFSRPLTRWSYALARLTVIVGMLSVVTWIPGLILFGLQVGLA
                     GGWWFLANWTLGAGLVVGFLLWLLILGLVAMASSAYVKWRVVAGAISLAFFFILSGVS
                     TMIDAVFRVNWGHMLDPAWAVNQVWRTLLGVNPMDGPGAWASMVSLTMFVLLLVLVIE
                     RKLRPVEVVR"
     gene            complement(1864..2844)
                     /locus_tag="AC16CY_00960"
     CDS             complement(1864..2844)
                     /locus_tag="AC16CY_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3352371.1"
                     /translation="MDTIPVVELDNLKVTLGRREILHGITCRLGVSGKGKAVGLLGPN
                     GAGKSTLILTLLGFLKPSAGSARILGMNCRHNMGEARSRIGYMPENDSFVGEMTAVTF
                     LRQMAELSGLPPRAALEKAHEVLFHVGLGESRYRQLRTYSYGMKQMAKLAQAIVHGPE
                     LVVLDEPTNGLDPAARRRMLKLVADMKEEHGMNVLICSHLLRDIEQVCDEAVILKDGA
                     IVHHCNLEEERRSNRSFVELEVTGDDRNLRMVLPEIGAEGVSEGGGRWRIVLPPGVEV
                     EAIWGLAARQNLLVRKLTHRRDTLEEIFLKAMGHLAQTPGESALADAGRA"
     gene            complement(2900..5266)
                     /locus_tag="AC16CY_00965"
     CDS             complement(2900..5266)
                     /locus_tag="AC16CY_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352372.1"
                     /translation="MGLGLGLLGSAFTLLNAYLLKPIDLPDPRALYSLSWDTDSTRRH
                     RFSLTDYEALQPEARRFAALAAAQDVTVMQDSVSRMGLLVTGNYFELLGARPALGRLL
                     RLDDAAVRGGVAVVVLSHQTWRSRYGSNPAIVGQRISLGRQRFEVVGITEPHAYLPGQ
                     DRVSFWAPLTMAGAFPGVDPWSEPDAAALVVVGRLRQDATAASVHAWLETWLRQRFPP
                     PSDLAPVAVRIDSLATRIPLNGVTLTMFVFIMSAFGLVLLVASANVTNLMLARALARQ
                     PEVALRLALGASRWRVARQLIVESLLLAIPAAATGLALVIVTTRVFAAVILATVPGDL
                     VPVENILVPLDPDWRVMAFLASAAVLSAVLITLAPAGRLAGMRLAHASRGEVSSDARG
                     SRLRSGLVAMQIGACALFLVGTVALLDEASRLANPRPNLAYERVSLIGIDPKVRDAVA
                     TRLASDAAVEQVAVAWKPPLFAGPLPTARVTASATSIALNAGYTGVSAEYFALFDIQI
                     VRGRTFTAAEATAGAPVALVSEATAAALWPGLDPLGQTLDLAAVPDGVPRKADGSVNM
                     SAPAPRPRGRVQVIGVTEDVVSGNIFDPVDVSCVYFPTSVQALTEMSLLVRTRTDDVE
                     ALRSAVTTAVKEVAPEMPFQVLPIRTMAGLAVWIFQAFSVAASVLSVVGLLFAYSGTH
                     AVVSFVVAQRTREFGVRMALGASAWRIVRGMLIETSRTASIGLAAGLAVAAGLIGLIR
                     ASVSILPDFGARPFVEGATIVLVATAVAAVVPLRAAARIDPAQALRTE"
     gene            complement(5574..>7427)
                     /locus_tag="AC16CY_00970"
     CDS             complement(5574..>7427)
                     /locus_tag="AC16CY_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HIR3352373.1"
                     /translation="AWSGQAIHLQRGDVIVKAAKQRRGRLRVLTRDSIASVRGTVFAV
                     SAGMGGSVVSVVEGSVVVNQPGREVILSPGEQASSIPSLASSVAEAVAWSPEANYYLE
                     LLASFAKIERDLASLPTELRTNSALLSYLPAGAVVYGSIPNPGVTIDRALFLAEEQAA
                     QNASFGAWWNSETGQQLRQMTDRIQSVNPLLGDEIVFCASVTGVGEPVPIVMARVQPG
                     RRAELASALEGLFAAAGEAPASYSVSDELMVVSSSHSNLAWALAHLQQGAGSPFATAL
                     GERYRRGVGWLIGMDAPPLVAMAAGDDAPPVELAGLIGMKYLFLEERAPAGALENEVT
                     FAFQGQRTGMGSWLADAGSGGAAEYLPVDALFAGYVSTREPLQLFEEFTAQITRADPS
                     FEQGLAEMDAKLGAGFVQNLTAALGTEAALAVTGFSTSGPTWVVAHLANNPPVIDFSL
                     QRLVEVFNAELGPDEQDKRIVLEEETTGGRTWITIRPGGLPIGFTWTYDGGYMVAASD
                     RAVAERAIATRNGGSQLVWSPAFLGQLPSSAGLHPSAFGWLNTKGALGILSALNPSPA
                     LKELVAGRDPILVVFDGTPEMIHAASRTRITGLIMDVMLIERLSQGTASPN"
BASE COUNT         1340 a         2557 c         2380 g         1150 t
ORIGIN      
        1 gcgaggtcga aagcccttgc ccgatctgac ggggaacgcg gcgtcacgac tggcccccga
       61 tcagatattc gtacagcgcg tccacgttct cgtcggccgg taccacgctc tcgatcctgt
      121 ggccgtcgag cgcgatgcgc ccgagcgctc gcgcgaactg ttcccggtcc cgggtcatca
      181 ccagcatgcc gagccggtcg tcgttcagct tgatttcggt gatgtggtcc tcgctgaaca
      241 gcagcgccgc cacgccggat gcgtcgcggc cgcggcacct cacgatgaac tggctgggat
      301 gctcctggat ctcgtcgcgc accttgcgga tctgcccttc ggccacgatc atcccgttgg
      361 cgatgagcac gacctgatcg ctgatcaggt ccacctcctg aagcacgtgg ctcgagatga
      421 tgacgtgctt tccctccgag gccagagagc ggaacaggtc gatcgtctcc gcgcggacca
      481 gtgggtcgag gccgttcagc ggctcgtcga gcaccagaag gtcgggatcg tgcgcgatgg
      541 cctgcgccag ccgcacccgt tgacgcatgc ccttggaata ccccgcgacc ttgcggttcg
      601 ccgcgtcggt cagtcccagg cgctgcagcg ccttccacgc ccgctcctcc gcgtccgatc
      661 gaccgtatcc gaaaagaagg agcccggtgg tgatgaaggt gaacccggtg gcccagcgcg
      721 gagcgctgtc gtactgcgtg gcgtatcccg tgattcgcat cagcgtttcg ggatcgcgcg
      781 gcgagatgcc gcgcatgagg atgctgccgt ggtcggggaa gatgagcccc gtcatcaggt
      841 tcatcagcgt cgtcttgccg gagccgttcg gcccgaccag gctcgtgata ccaggcggga
      901 tgttcagcgt gacccggttc acgccgagca cctcgccgta gaacttcgag acctcgtcga
      961 agacgaccgc cggttcgctc accggaccac ctccaccggc cgcagctttc gctcgatgac
     1021 caggacgagc aggagaacga acatcgtcag cgacaccatg gaggcccagg cccctgggcc
     1081 gtccatcgga ttcactccca gcagcgtgcg ccacacctgg ttgaccgccc aggccggatc
     1141 cagcatgtgt ccccaattca cgcgaaagac cgcgtcgatc atcgtggaca cgccgctcag
     1201 gatgaagaag aaggcgaggc tgatcgcccc ggccacgacc ctccacttca cgtaagcgga
     1261 gctggccatt gcgaccaggc cgagaatcag cagccagagc agaaacccca caaccagacc
     1321 ggcgcccaac gtccagttcg ccaggaacca ccagccgccg gccaggccca cctgcagccc
     1381 gaacaagatc aggcccggaa tccatgtgac gaccgacagc atgccgacga tcacggtcag
     1441 ccgtgccagg gcgtagctcc agcgcgtgag cggccggctg aagtacagcg ggagcgcgtt
     1501 gttcgccagg tccggcgcaa gcaggccagg ccccgccagc gcagtgagga agaccgcgca
     1561 ggctccctgg acgtacatga atatcgagaa gaacctgccg tccacctgga tgaactcgcg
     1621 aaactccggt tcgagcccct ggagcagctg cacatgattg gtgagataga tgaagccggc
     1681 gcacaagagg ggccagacga acgccaacgt ggtgagcagg aggaccaggc gctgctgata
     1741 gagctccttc caggcgtagc ggggaaggac catgaaccgc gtccagcgcc ctgtgagggt
     1801 tcccgcgtat cgccgatatc cccgcttgta aaccgccatc cctgtctctc taccgcgccc
     1861 gcatcacgcg cgtcccgcgt cggccagcgc gctctctccg ggtgtctgcg cgagatggcc
     1921 catcgctttc aggaagatct cttccagcgt gtcccggcgg tgcgtgagct tcctcaccag
     1981 caggttctgc cgcgccgcga gtccccagat agcttcgacc tcgactcccg gcggcaggac
     2041 gatacgccag cgcccgccgc cttcggacac gccctccgct ccgatctcgg gcaaaaccat
     2101 gcgcaggttc cgatcgtcgc cggtgacctc gagctcgacg aaagacctgt tcgacctccg
     2161 ctcttcctcg aggttgcagt ggtgcacgat cgcgccgtcc ttcaggatca ccgcctcgtc
     2221 gcacacctgc tcgatgtcgc gaagcaggtg ggagcagatg agcacgttca tcccgtgctc
     2281 ctccttcatg tcggcgacga gcttgagcat tcgccggcgc gcggccggat cgagcccgtt
     2341 ggtcggttcg tcgagcacga ccagctccgg cccgtggaca atcgcctggg cgagcttggc
     2401 catctgcttc atcccgtacg aataggttcg cagctgccgg taccgcgact ccccgagccc
     2461 gacatggaac aggacctcgt gcgctttctc gagcgccgct cgtggcggga ggcccgacag
     2521 ttccgccatc tggcgcagga aggtcactgc cgtcatctcg cccacgaacg agtcgttctc
     2581 cggcatgtag ccgattcgcg accgggcctc gcccatgttg tgtcgacagt tcatgccgag
     2641 gatacgggcg ctgccggcgc tgggcttgag aaagccgagg agcgtcagga tcagcgtgga
     2701 tttgccggcg ccgttcggtc cgagcaaccc cacggccttt cctttgcccg aaacgccgag
     2761 acggcaggtg atcccgtgca ggatctcccg ccgtcccagt gtgaccttca ggttgtcgag
     2821 ttcgacgacc ggtattgtgt ccaaggctct ttccatcgaa caacacgaga atcggatcgc
     2881 gtccggcgcc gttgtcctgt cactctgttc gaagggcctg cgcaggatcg atgcgtgcgg
     2941 cggcgcgcag tggcaccacc gcggccacgg ccgtcgcgac gagcacgatc gtggcgccct
     3001 ccacaaaagg acgggcaccg aagtccggca ggatcgaaac tgaagcgcgg atcagaccta
     3061 tcaggccagc cgcgaccgcc aaccctgcgg ccaggccgat cgaggccgtg cgagacgtct
     3121 cgatcagcat cccccgcacg atccgccagg cgctggcacc gagcgccatc cggacaccga
     3181 actcgcgcgt ccgctgagcc acgacgaacg agacgacggc atgcgttccc gagtacgcga
     3241 agagcaggcc cacgacactg agtaccgacg cggctaccga gaaggcctga aagatccaga
     3301 cggcgaggcc ggccattgtt cgtatcggga gcacctgaaa tggcatctct ggcgccacct
     3361 ccttcacggc ggtggtcaca gccgaccgca gtgcctcgac gtcgtcggta cgggtccgga
     3421 cgagcagcga catttccgtg agcgcctgca cgcttgtcgg gaaatagacg cagctgacat
     3481 cgaccgggtc gaagatgttg ccgctgacca cgtcctccgt cacgccgatg acctgcacgc
     3541 gaccacgcgg ccggggcgcg ggagcggaca tgttcacgct gccatcggcc ttccgtggaa
     3601 ctccatcggg gaccgcggca aggtcgagcg tctgcccgag cgggtcgagg cccggccaca
     3661 atgccgccgc cgtcgcttcg ctgacgagcg ccaccggagc tcccgcggtg gcttcggccg
     3721 ccgtgaaggt gcgcccgcgc acgatctgaa tatcgaacag cgcaaagtac tcggccgaca
     3781 cacccgtgta gccggcgttc agtgcgatgc tcgtggccga tgccgtcacc cgggctgtgg
     3841 gcagcggtcc cgcgaacagc ggaggtttcc acgccaccgc gacctgctcg accgcagcgt
     3901 cggacgccag gcgtgtcgcg accgcgtctc gcaccttcgg atcgatgccg atgagcgaca
     3961 cccgctcgta tgcgagattg ggccgcggat tcgcaagacg cgacgcctca tcgagcagtg
     4021 ccaccgttcc gacgagaaac agggcgcatg cgccgatctg catggccacc agtcccgatc
     4081 gcagacggga cccacgcgcg tcggaagaca cttcgcctcg cgaggcgtga gccagacgca
     4141 tacccgcgag gcgcccggcg ggagcgagcg taatgagcac cgcggacagt accgccgccg
     4201 aggcgagaaa cgccatcacg cgccagtcgg gatcgagtgg aaccagtatg ttctcgaccg
     4261 gcaccaggtc accaggaacc gtggcgagga tgacggcggc aaacacacga gtcgtgacga
     4321 tgacgagggc aagccccgtg gcggcggcgg gaatggcgag cagcaggctt tccacgatca
     4381 actggcgcgc cacccgccac cggctggcac caagcgccag gcgcagggcg acttccggtt
     4441 gtcgtgccag cgcgcgcgcc agcatcaggt tcgtgacgtt cgcggaggcg accagcagca
     4501 ccaggccgaa cgccgacatg atgaacacga acatcgtcag cgtgaccccg ttgagaggga
     4561 tgcgcgtggc gagcgagtcg atgcgcaccg ccactggcgc gagatcggac ggtgggggaa
     4621 aacgctgacg tagccacgtc tcgagccacg catgcactga cgctgcggtc gcgtcctgtc
     4681 gcaggcgccc gacgacgacg agcgccgcgg cgtctggctc ggaccatgga tcgacgcccg
     4741 gaaacgcgcc cgccatcgtc aacggtgccc agaagctcac gcggtcctga ccgggcaggt
     4801 acgcgtgcgg ctccgtgata ccgacgacct cgaagcgctg acgtcccagc gagattcgtt
     4861 gcccgacgat ggctggattc gagccgtacc gggagcgcca cgtctggtgc gacagcacga
     4921 cgaccgccac cccgccgcga actgccgcgt catccagtct cagcaatcgc ccgagggccg
     4981 gccgcgcccc gagcaactcg aagtagttgc cggtcacgag cagtcccatc ctggagacag
     5041 agtcctgcat gaccgtgacg tcctgcgcgg ccgccagtgc ggcgaagcgg cgcgcctccg
     5101 gttgcagcgc ctcgtagtcc gtcagcgaaa accggtggcg gcgcgtgctg tcggtgtccc
     5161 aactgagcga gtacaacgca cgcgggtcgg gcaggtcgat gggcttgagg aggtaggcgt
     5221 tgaggagcgt gaaggcggag cccaacagcc cgaggcccag tcccattgtt gccgcgactg
     5281 tcgccgcgaa gagcggcgct cgccggaggg tccgagtcgc gtagcggatg tcttggaaga
     5341 gtgagtcgac gcccactaca aggctccctg atcgcggtgg ccgatcgtgg ccacgagaac
     5401 gcttacgtgg atgctacgag gttcgttccg ccagaacacg cagtgatcac cgtgtgcccg
     5461 aaacgccgag acggcaggag atcccgtgca ggatctcccg ccgtcccagt gtgaccttca
     5521 ggttgtcgag ttcgacgacc ggtattgtgt ccaaggctct ccaggcctgg ccgtcaatta
     5581 ggggacgcgg tcccctggct caggcgctcg atcagcatca cgtccatgat caacccggtg
     5641 atacgagtac ggctggccgc gtggatcatt tccggcgtcc cgtcgaacac gacgagaatc
     5701 ggatcgcgcc cggccacgag ctccttcagc gccgggctcg ggttcaacgc cgagaggatt
     5761 ccgagtgccc ccttcgtgtt cagccagccg aaggcggacg ggtggagccc ggcagacgac
     5821 ggaagctggc cgaggaacgc cggggaccag acgagctgag atccgccgtt ccgggtggcg
     5881 atggcacgct ccgcgacagc gcgatcggag gccgccacca tgtaaccccc atcgtatgtc
     5941 caggtgaaac cgatcggaag tcctcccggt ctgatcgtga tccaggttcg tccacccgtg
     6001 gtttcctcct ccaacacgat ccgcttgtcc tgctcgtcgg gcccgagctc cgcgttgaac
     6061 acctccacca gcctctggag agagaagtcg atgaccggcg ggttgttcgc caggtgcgcc
     6121 acgacccacg tcgggccgct ggtcgagaat cccgtcaccg ccaaagccgc ttcggtcccc
     6181 aacgccgccg tcaggttctg gacgaagccg gcgccgagct tcgcgtccat ttcggccagg
     6241 ccctgttcga agctcggatc cgcgcgcgta atctgcgcgg tgaactcctc gaacagctgc
     6301 aatggctcgc gggttgacac gtagcccgcg aagagggcat cgactggcag atactccgcc
     6361 gcacccccgg agcccgcgtc agccagccac gaccccattc cggttcgctg gccctggaac
     6421 gcgaacgtga cctcgttctc cagcgctccc gccggagcac gctcctcgag gaagaggtac
     6481 ttcatcccga tcaagccggc gagctcgacc ggcggggcgt cgtctcccgc cgccatcgcg
     6541 accaagggag gcgcatccat cccgatgagc cagcccacgc cgcgtcgata ccgctcccca
     6601 agcgctgtcg cgaacgggga gccggcgccc tgctgcaggt gagcgagggc ccacgccagg
     6661 ttcgaatgcg agctcgagac gaccatcagc tcgtcggaaa ccgaatacga tgcgggagcc
     6721 tcgcccgcgg cggcgaacag cccttccagg gcgctcgcga gctccgccct ccggccgggt
     6781 tgcacacggg ccatcacgat cggcacgggc tcgccgactc cggtgacgct ggcgcagaag
     6841 acgatttcgt ccccgagcag cgggttcacc gactggatgc ggtccgtcat ctgtctcaac
     6901 tgctgtcctg tctcggaatt ccaccaggct ccgaagctcg cgttctgggc cgcctgctcc
     6961 tcggcgagga aaagggcccg gtcgatcgtg acgccaggat tcgggatcga gccgtagacg
     7021 acagcgcccg cgggcagata cgagagcagg gcagagttgg tccgaagctc ggtggggaga
     7081 ctcgccagat cgcgttcgat cttcgcgaac gacgcaagca gctccaggta gtaatttgcc
     7141 tcagggctcc aggcgacggc ttccgctacc gagctcgcca gcgacgggat cgaggaggcc
     7201 tgctcacccg ggctgagaat cacttctctg ccaggctggt tcaccaccac cgatccctcg
     7261 accaccgaaa ccaccgagcc gcccatgccg gccgacaccg cgaagacggt tccccttacc
     7321 gaggcgattg aatcgcgcgt cagcactcga aggcggccgc ggcgctgttt ggcggccttg
     7381 acgatgacgt caccgcgctg gaggtgaatc gcctggccgc tccaggc
//
LOCUS       DBNGSH010000048         7885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3908229, whole genome shotgun sequence.
ACCESSION   DBNGSH010000048 DBNGSH010000000
VERSION     DBNGSH010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3908229"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="AC16CY_00975"
     CDS             <1..636
                     /locus_tag="AC16CY_00975"
                     /inference="COORDINATES: protein motif:HMM:NF020671.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB domain-containing protein"
                     /protein_id="HIR3352374.1"
                     /translation="RRGLAVSIGGVLVSAARLASVEDALVAAVGRYHSGHPLEEGIPR
                     EELRERLFSGAPIAVFEEVLRGLAARSVILDRDRVARAGHRVALTEQESRVRDAMVEA
                     VKAGGLAPPDATAVAAAAGAPPGMIDRLATLLVRQGVLVRVGDLLFHQSALERLKQEI
                     QSMKREGTIDTIDVATFKSRYNVTRKHAIPLLEFLDRERVTRRVGDARRIL"
     gene            633..2405
                     /locus_tag="AC16CY_00980"
     CDS             633..2405
                     /locus_tag="AC16CY_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3352375.1"
                     /translation="MKAQDLVLLGGGHSHVAVLRSFGMDPLPGVRVTLISRGVDTPYS
                     GMLPGLIAGHYSYDEAHIDLQPLARFAGARAIFDEAVGLDLANRQVLFDKRPPISYDV
                     LSIDIGSTPHLDVPGSAANAVPVKPISRFLDRWSLLCERLRKGDRRRLAVVGGGAGGV
                     ELLLAVQFRMRTLREGDGKGDATLEYHLFTNSDQVLPTHNRSVRRRFERVLAERGVVV
                     HAGSAVVEVERSRLRTADGRSHDVDDVLWTTEAAAAPWLAQSGLAVDEAGFVRVSGTL
                     QSISHPEVFASGDIAAMVDHPRPKSGVFAVRQGPPLARNLRDTLLGEPLETYRPQRRF
                     LSLITTGDQYAVASRGIWAFEGRWVWRWKDWIDRRFMRVYQNLPDMKAPAAGEPASAS
                     ALLRLVHWLVAKPWLYNVAQNLAGAEAVRRHLRPRLQETSGKLVLDVGAGTGLYLSCF
                     PPSARYIWLDADPQKLAGFRGTPGAARRALLGDATCLGLRRDAVDVAVCTNVTHHLDE
                     KQFALCIDELARVVRGKLILHDAVKTARPVSRLLWGLDRGSHPRSREALLAAVERRFT
                     IEQVERYSTWHEYVLLVAVPRGHG"
     gene            complement(2623..3660)
                     /locus_tag="AC16CY_00985"
     CDS             complement(2623..3660)
                     /locus_tag="AC16CY_00985"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR3352376.1"
                     /translation="MDVQTLESRRVTTAVSSNYVRRPPLFIVRCLIGVVMFGVAAPAL
                     WLVHRLGLTVRLASVVSRRQRQKTAAKNPFTGYTPTSHDVFVATFAKSGTNWMMQIAH
                     QLLFHGEGHFDHIHEVVPWPDSKLMGPMRGYAIPLEDPAVWMASPEKKRVIKTHYSWD
                     LVPYSEDARYITVIRDPKDVFVSGYFFFLMNAFGGAVTPSVETWLDLFLSDFFLINGS
                     WAANTAGYWAQRHRKNVLICSFKAMRQDLPGSVRTVAAFLGVRASDEVIARVCERSSF
                     DYMKKNDSQFRTWTLVPWSREGPMIRRGSQGSSSELLTPAQQRRIDDHFRSELKRLGS
                     DFPYDEFCDVA"
     gene            complement(3798..4301)
                     /locus_tag="AC16CY_00990"
     CDS             complement(3798..4301)
                     /locus_tag="AC16CY_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3352377.1"
                     /translation="MALRHSESSALIDASPEAAFAFIDDHARFSSHMNESSWMMGGGR
                     MSVGLDAAKGQAVGSHISLSGRVFGVRLSVDEVVTRREPPINKVWETVDVPRLLVIGA
                     YTMGVQITPVDGRSRMRVFIDYELPKGWATYWLGLLFSGFYARWCVDQMLKGAVKTFA
                     SPRPVAA"
     gene            5045..5734
                     /locus_tag="AC16CY_00995"
     CDS             5045..5734
                     /locus_tag="AC16CY_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352378.1"
                     /translation="MRTTSIRVLAGAAIAFAALANTANAQTGSPILNVLEVQKLVAST
                     EPADNARLGAHFAALADRYTREATRHNAMAQAFIAAPTRRTSANTAADHCKRLGALNK
                     QSADTLRELAAYHEKQAAGVVAAPPKGAARFQAGAGAPEPADDQLSTLAARASTPADH
                     HALEEYFQTTAKRYREAVNEHSSMAQAYRGTRIAQAAVHCDRLVGLSRDEAKEATAAA
                     EMHKQLATVPR"
     gene            5801..7057
                     /locus_tag="AC16CY_01000"
     CDS             5801..7057
                     /locus_tag="AC16CY_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3352379.1"
                     /translation="MNGFSSSIVQLVVMLIASPAVVAAQDSKPLPSPLALADVIRIAG
                     ERRDEIQAARARTRAGEVRPVIVSALPDPMLSPSLDHLPFMFNGADISVVIEQQIPLS
                     GIRGHRRASSLADLDRLNAETKRTTLDVGLEAANAYLMVQERRRTAALLDEQVAFARD
                     VVSAANARYASGTAPQSDVLRAEVEVARLEATARALGGDVRGAEAMLNTSLALDADTP
                     VPPLSTLTFAQPVPSWPAIRAALTSRPELAAGRADIARAEAEVQVMRDMFRPMATIRT
                     GPSYTMAEGRGWMAMVGLSLPIWRSKLKAGVAEAEAMRAMSEADLRAMTRMIEGDAAA
                     AISQLQGARDRQTALTTDVLPRARMAIAPAVAGYGAGQLPLVSVIEAIQALWAVQSDL
                     IAADTQLGLAWARLGRAIGSYEAIVQ"
     gene            7054..>7885
                     /locus_tag="AC16CY_01005"
     CDS             7054..>7885
                     /locus_tag="AC16CY_01005"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3352380.1"
                     /translation="MTPPATLRRIGAPVASAMATALVFIVGLWVQHARSSWPFDRPVD
                     TSHVESMAGMEPTTLRDPQGRPESGRGTSGATTNDRVPVDVAAARSLDIRLETVGRQS
                     LTQTVRAVATIVPDESRISHVHTRVAGWIEHLDVNTTGEEVRAGQPLAHIFSQELLSS
                     QTEYLAVRRNTAASGITSSVVASGRTRLGVLGMTPEEIDAIEHTGEPRRLVTVVAPRS
                     GVVVNRGITVGTSVDPSTTLLTIADLSHVWVLAEVPEASIPSVRVGSKALLDFPASGR
                     S"
BASE COUNT         1364 a         2588 c         2613 g         1320 t
ORIGIN      
        1 cggcgcggcc ttgccgtctc gatcggcggc gtgctcgtct ccgccgcgcg gctcgcatcg
       61 gtcgaagacg cgctcgtcgc cgccgtcggc aggtaccact cgggccaccc gctcgaggag
      121 gggatcccgc gggaagagct gcgcgagcgg ctgttttccg gcgcgccgat cgccgtgttc
      181 gaagaagtgc tccgcggcct cgccgcgcgg agcgtcatcc tcgatcgcga tcgggtggcc
      241 cgcgctggac accgcgtcgc gctgacggaa caggagtctc gggtgcgcga tgcgatggtc
      301 gaggcggtga aagcgggcgg cctcgcgccc ccggacgcca cggccgtcgc ggccgcggct
      361 ggcgctcctc cgggcatgat cgaccgcctc gcgacgctcc tcgtgcgaca gggcgtgctc
      421 gtccgcgtgg gcgatctgct gttccaccag tcggccctcg agcgcctcaa gcaggagatt
      481 cagtcgatga agcgggaagg cacgatcgac acgatcgacg tcgccacgtt caagagccgg
      541 tacaacgtga cccggaagca tgcgattcca ctgctggagt ttctcgatcg agaacgcgtg
      601 acgaggcgag tgggagacgc tcggcgaatt ctttgaaggc gcaggatctg gttctcctgg
      661 gcggcggcca cagccacgtg gccgtcctca ggagcttcgg gatggatccg ctgccgggcg
      721 tccgcgtgac gttgatctcg cgcggggtcg acacgccgta ctccggcatg ctgccgggcc
      781 tcattgccgg acactactcc tatgacgagg cccatatcga cctgcagccg ctggcccggt
      841 ttgccggagc gcgggcgatc ttcgacgaag cggtcggcct cgacctggcg aacaggcagg
      901 tgctcttcga caagcggccg ccgatctcgt acgacgtgct ttcgatcgat atcggctcga
      961 cgccgcacct cgacgtgcct gggtcggcgg cgaacgccgt tccggtcaag ccgatctcgc
     1021 gattcctgga tcgctggtcg ctgctctgcg aacggctgcg caagggggat cggcggcggt
     1081 tggccgtcgt cggcggcggc gccggaggcg tggagctgct gctggcggtg cagttccgca
     1141 tgcggacgct gcgtgaaggc gacgggaagg gcgatgcgac gctcgagtac cacctgttca
     1201 ccaacagcga ccaggtgctg ccgacgcaca acaggtccgt ccgccgcagg ttcgagcgcg
     1261 tgctcgccga acgcggcgtc gtcgtacacg caggcagtgc cgtcgtggaa gtcgaacgct
     1321 cgcggcttcg caccgccgac ggccgctcgc acgacgttga cgacgtgctc tggacgacgg
     1381 aagcggcggc ggcgccctgg ctcgcgcagt ccggtctggc ggtagacgag gctggattcg
     1441 tgcgcgtctc gggcaccttg cagtcgatct cgcacccgga ggtgtttgcg tctggtgaca
     1501 tcgcggccat ggtcgatcac cctcgtccga agtcgggcgt gtttgccgta cggcagggcc
     1561 cgccgctggc gaggaacctg cgtgacacgc tgctcggaga gccgctcgaa acctatcggc
     1621 cccagcggcg gttcctgtcg ctcatcacca ccggcgacca gtacgcggtc gcctcgcggg
     1681 gaatatgggc gttcgaaggc cgatgggtct ggaggtggaa ggactggatc gatcgccgat
     1741 tcatgcgtgt gtaccagaac ctgccggaca tgaaagcacc ggcagcgggt gaacctgcgt
     1801 cggccagcgc cctcttgcgg ctcgtccact ggctggtcgc aaagccgtgg ctgtacaacg
     1861 tggcgcagaa tctggcgggc gccgaggcgg tgcgccggca tttgcggccg cggctccagg
     1921 agaccagcgg caaacttgtg ctggacgtcg gcgccggcac aggcctgtac ctgtcctgct
     1981 ttcccccgtc cgcccgctac atctggctcg atgccgatcc gcagaaactc gccgggttcc
     2041 gaggcacgcc cggcgccgcg cggcgcgccc tgcttggcga cgccacgtgt ctcggtctgc
     2101 ggcgagacgc cgtggatgtc gccgtctgta cgaacgtcac ccatcacctg gacgagaagc
     2161 agttcgcgct gtgcatcgac gaactggcgc gcgtcgtccg tggcaagctg atccttcacg
     2221 acgccgtcaa gaccgcgcgg cccgtgagcc ggctgctgtg ggggctcgac cgcggaagcc
     2281 atcctcgcag ccgagaagcg ctcctggctg ccgtcgagag gcgattcacg attgagcagg
     2341 tcgagcgtta ctcgacctgg cacgagtacg tgctgctcgt ggccgtgccc aggggtcatg
     2401 gctagagcta cacgccttga gtgaggtctg tcgttcacac ttctcgcttc cgagcttctc
     2461 ggttcgtgtt cacgttcggc gttcgcgttc aggttcctgg gaacaccgaa cacccgcaca
     2521 gcgaactccg aacacgaacc tgaaaaactg aacacgcacc gagaagcgag aacagagaag
     2581 cgcgaacgac agatctcact ctcgattgac tgaatcctgg gatcaggcga cgtcgcagaa
     2641 ctcgtcgtag ggaaagtcgg atcccaggcg cttcagctcg ctcctgaagt ggtcatcgat
     2701 cctgcgctgc tgcgcggggg tcagcagttc cgacgagctc ccctgagatc ccctgcggat
     2761 catcggcccc tcgcggctcc agggaacgag cgtccacgtc cggaactggc tgtcgttctt
     2821 cttcatgtag tcgaacgacg accgctcgca cacgcgtgcg atcacttcgt cggaggcgcg
     2881 gacaccgagg aaggcggcta cggtgcgaac cgagcccggg agatcctgtc gcatcgcctt
     2941 gaacgaacag atgaggacgt tcttgcgatg gcgctgggcc cagtagcccg ccgtgttcgc
     3001 cgcccacgag ccgttgatga ggaagaagtc cgacaggaag aggtcgagcc acgtctcgac
     3061 cgacggcgtc accgcccccc cgaatgcgtt catcagaaag aagaagtaac ccgagacgaa
     3121 cacgtccttc gggtcccgga tcaccgtgat gtatcgcgcg tcttcggagt acggcaccag
     3181 gtcccaggag tagtgcgtct tgatgaccct tttcttctcg ggagatgcca tccacacggc
     3241 gggatcctcg agcgggatgg catagcctct catcgggccc atcagcttcg aatccggcca
     3301 cggcacgacc tcatgaatgt ggtcgaagtg tccctctccg tggaagagga gctgatgggc
     3361 gatctgcatc atccagttcg tgccgctctt cgcgaaggtc gcgacgaaca cgtcgtgcga
     3421 cgtgggcgtg taccccgtga acgggttctt cgcggcggtc ttctgacgct gtcgccgcga
     3481 cacgacggac gcgagccgca cggtcagccc gagacgatgc acgagccaga gagcaggcgc
     3541 ggccacgccg aacatcacca cgccgatcag acacctgacg atgaacagcg gcggccggcg
     3601 gacatagttc gaggagacag ccgtggtcac gcgtcgtgac tcgagcgtct gtacgtccac
     3661 ggctacatcc tagcgttgac gtcgacgagg ccaaagcata gccacgacga cacgaaggtc
     3721 acggaccgac agccacgaag ggctgattca tgcctccatg acgcgaggac cccgccgctc
     3781 gattacatct ggcaccctca agcggcgacg ggacgcggtg aggcaaaggt ctttacggct
     3841 cccttgagca tctgatcgac gcaccatctg gcatagaagc cgctgaagag cagccccagc
     3901 cagtaggtgg cccatccctt cggcaactcg tagtcgatga acacccgcat gcggctcctg
     3961 ccgtctacgg gggtgatctg aacccccatg gtgtaggcgc caatcacgag cagccgcggc
     4021 acgtcgacgg tctcccacac cttgttgatt ggcggctccc gccgagtgac gacttcgtca
     4081 acggacaggc gaacgccgaa tacgcgaccg ctcagagaaa tatgcgatcc cacggcttgc
     4141 cccttggctg catcgagccc cacggacatc cggccgcctc ccatcatcca cgacgactcg
     4201 ttcatgtggg acgagaaccg cgcgtgatcg tcgatgaagg caaacgccgc ctccggggag
     4261 gcgtcgatga gggcgctgct ttcagagtgg cgtaacgcca taactcctcc agatgctctg
     4321 cacagcagct ctggtgccag gccctgcccg ccggcccggt ggcctcgttt agcttgccgg
     4381 aacacgcggt gtggaatttt cggcaccgtc ttggcgaaat tccgcaggct cgaagaaggc
     4441 agatcgtcga cacgtcaaga aggcgctgcg attcacgggt gtcgccgccg ggcggcctgg
     4501 cgcgggtctt gcccctgagg gtcgagagca cgaacgtgtt tcgagcagcc cttctttcga
     4561 ccgtgttgac gctggccgcc gggcagaacg ccgccgctct gttgtgcggt gtttggtgtc
     4621 actccggtgg aggaatgacc ggcgcgtgcg agcaccaaac ggaggccgcg ccgccggaca
     4681 tcatcgccaa cgacggctgt acggtcacca gcaacgcgac cgtgtacgtg cgcgaggacg
     4741 ttcgccgtag cgtctcggcc ccgactgttc tcagcggagt cccggttccg cggttcgtgt
     4801 tcactccacc ggcttccagc agcctttcag ggtacgaggc tgcccgccgg gcggagctcg
     4861 atttccgacc tctcgttctg gccctccgaa tctaagtcgc gttgccgcat ccggtcgtgg
     4921 accggacagg gcattgccgc acaacaggcc tcgatcgaag ggcgggcggc aaagcccgac
     4981 tctcgacctg gtccgaacat gcaactcgca gcgtgacgaa ggtcacagaa aaggcaggtc
     5041 cgtcatgaga acgacttcga ttcgggtgct cgcgggcgcc gcgattgcgt tcgccgccct
     5101 tgcgaacacc gcgaacgcac aaaccggctc gccgattctg aacgtcctgg aagttcagaa
     5161 actggtggcg agcacggagc cggcggacaa cgcccgtctg ggtgctcatt tcgccgccct
     5221 ggccgatcgc tacacgcgtg aagccacacg tcacaacgcc atggcgcagg cattcattgc
     5281 ggcgcccacg cgacgaacgt ccgccaacac ggccgccgac cactgcaagc gtctgggcgc
     5341 gctcaacaaa cagtcggcgg acacgctccg cgagctggcg gcctatcacg agaagcaggc
     5401 cgcaggcgtc gtggccgcgc caccaaaagg agccgctcga ttccaggcgg gggccggtgc
     5461 gcccgagcct gcggacgatc agctcagcac actcgccgca cgtgccagca cgccagcgga
     5521 tcaccacgca ctggaggagt acttccagac gacggcgaaa cgctaccgcg aagcggtgaa
     5581 cgaacactcc tcgatggcgc aggcctatcg gggtacgcgg atcgctcaag cagcggtcca
     5641 ctgcgatcgt ctcgtgggct tgtcgcgtga cgaagcgaag gaagcgacgg cagctgcgga
     5701 gatgcacaag cagcttgcca cggtccctcg gtaagagcgc ccggaggcac gggccggcgg
     5761 gtcgccggcc cgcctcgacc tgttcgagtc ggggattgat atgaacggct tttcttcctc
     5821 gatcgtgcaa ctggtggtga tgctgatcgc gtcacctgcg gttgtggccg cgcaggactc
     5881 gaagccgctg ccctctccgc tcgccctcgc cgatgtcatc cgcatcgccg gcgagcggag
     5941 agacgaaata caagcggcgc gtgcgcgcac gagagccggc gaagtgcgcc cggtgatcgt
     6001 atccgcactg ccggatccga tgctctcgcc atcgctcgac cacttgccgt tcatgttcaa
     6061 cggggcggac atcagcgtcg tcatcgagca gcagatcccg ttgtcaggca tccggggcca
     6121 tcgccgcgca tcctcacttg ccgatctcga tcgcctgaac gccgagacga agcgcacgac
     6181 gctcgacgtc gggctcgagg ccgcgaatgc gtacctgatg gtccaggaac gtcgtcgaac
     6241 tgccgcactc ctcgacgagc aggttgcctt tgcacgtgat gtcgtcagcg ccgcgaatgc
     6301 gcgatacgcc agcggcactg cgccgcaatc cgacgttctc cgcgccgaag tcgaggtggc
     6361 gcgactcgaa gcgacggcgc gcgcccttgg tggcgacgtg cgtggagccg aggcgatgct
     6421 caacaccagc ctcgcactgg acgccgacac cccggtacca ccattgagca ccctgacgtt
     6481 tgcccagcca gtgccgtcat ggccggcgat cagagcggcg ttgacgtcgc gccccgagct
     6541 ggccgcgggt cgtgccgaca tcgcgcgcgc tgaagccgaa gtgcaggtga tgcgcgacat
     6601 gttccggccg atggcgacga ttcgcaccgg accgtcctac acgatggccg aaggccgcgg
     6661 ctggatggcg atggtgggtc tcagcctgcc gatctggcgc agcaagctga aggcgggtgt
     6721 cgccgaagcg gaagcgatgc gcgcgatgtc ggaagcggac ctccgcgcga tgacgcgcat
     6781 gattgaaggc gacgcggcgg cggcgatcag tcagttgcaa ggggcacgcg accgtcagac
     6841 agccctgacg acggacgtcc tgcctcgcgc tcgaatggcg atcgcgccgg ctgtggcggg
     6901 gtacggggcg ggccagcttc ccctggtcag cgtcatcgaa gccattcaag cgctctgggc
     6961 tgttcagtcc gatctcattg ccgcagatac gcaacttggt cttgcctggg cgcgcctggg
     7021 tcgggccatt ggatcgtacg aggcgatcgt ccaatgacac ctccagccac gctccgacgc
     7081 atcggcgctc ctgtcgcatc ggcgatggcg actgcgctcg ttttcatcgt cgggttgtgg
     7141 gtgcagcacg ctcgatcctc atggcccttc gatcgtccgg ttgacacatc acatgtcgag
     7201 agcatggccg gcatggaacc aacgaccctt cgagaccctc agggtcgtcc cgagtccggt
     7261 cgagggacga gcggcgcgac tacgaacgac agggttcccg tcgatgtcgc cgctgcgcgg
     7321 tcgctggaca ttcggctcga gacggtcggg cgacagtctc tgacgcagac cgtgcgggcc
     7381 gtggccacga tcgtgcccga cgaatcacgg atttcacacg tgcatacccg cgtggcgggc
     7441 tggatcgagc acctcgacgt caacacgact ggcgaggaag tgcgcgcggg ccagccgctc
     7501 gcgcatatct tctcgcagga gttgttgtcg tcccagaccg aatacctggc cgttcgaagg
     7561 aatactgcgg cgtcgggaat tacgagcagc gtcgttgcga gcggcaggac caggctcggc
     7621 gtactgggca tgaccccgga agagatcgac gccatcgagc acacgggcga gccgaggcga
     7681 ctcgtgacgg tggtggcacc gcgtagcggc gtcgtcgtga atcgaggcat aacggtcggc
     7741 acgtcggtgg atccctcgac cacgctgctc acgattgcgg acctgtcgca cgtgtgggtg
     7801 ctggccgagg tccccgaggc gagcattccg tccgtccgtg tcggcagcaa ggcgctgctg
     7861 gactttccag cgtctggccg ctcgc
//
LOCUS       DBNGSH010000049         3134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_172973, whole genome shotgun sequence.
ACCESSION   DBNGSH010000049 DBNGSH010000000
VERSION     DBNGSH010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_172973"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="AC16CY_01010"
     CDS             complement(<1..435)
                     /locus_tag="AC16CY_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR3352381.1"
                     /translation="MSQRLGQRTASWLNAVGRSIWRIGWPVTDKDRAAVMISSIFLHI
                     HPARVSRHVLRPSATLGLGLIAAILFVVLSVTGLALMLYYVPYPPEAYRSMKDLQFVV
                     TFGVVLRNMHRWSAHAMVVVVFLHMCRVFWTGSYKAPREFNWV"
     gene            complement(448..906)
                     /locus_tag="AC16CY_01015"
     CDS             complement(448..906)
                     /locus_tag="AC16CY_01015"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR3352382.1"
                     /translation="MTEDQIDLQRRRIFAVGCFGTGAATAGFLAATFRFLVPNVLYEP
                     SRRFDIGSPAEFPPGSVTFLADRRLYVFNSADGFFAVSSECTHLGCNVRHAGAGFECP
                     CHGSRFDENGSVVAGPAPRPLAWYAINLSPRQQLIVDLDQTVGPEFRLRV"
     gene            complement(906..2813)
                     /locus_tag="AC16CY_01020"
     CDS             complement(906..2813)
                     /locus_tag="AC16CY_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3352383.1"
                     /translation="MSLRSYTAAVPDLRYFLNLIPCRTGCPVRTNAGGYVRAVAEWDT
                     ARGYRIARAPNPLASICGRICAHPCESNCRRGVIDQPISIRALKRTLTERHGVENTLS
                     GQPGPLEQLHLAVKGPDGPARIGVVGAGPAGLSCAHDLGLLGYRVTLFDSAPVVGGML
                     YQGVPEYRLSRDLIRAEVDRILTLGVELKLGWKLGRDFTVSDLRRNGYAAVFLALGTS
                     RGRELNIAGSDLDGVINGVDFLLNANLGYHVALGDRVIVIGGGNVAIDVARTALRYSA
                     AEGKPELPVTAEQLLQTWGYDNAFIDAARTALRLGARHVQLVSLESRLEMPASPDEVR
                     EAEEEGITLAPGLGPKAILGENGRAVGVETLDVASVFDSAGRFNPTFVPGSERRLAAD
                     TIILAVGQQPDPTCLAADAEIEIMPSGLVKVDPVTLATTMAGVYCGGDLAFGPRIVIS
                     AEADGKRAALAIHESLGGGRVPRARARFRPIELNRAGDPYDRIPRQAVPALPVARRTG
                     FREVDEGYSEEQARLEASRCLWCNVETIFDSELCILCSGCVEICPEACLTLVPASQLR
                     PSPDLDAVRDALGESDEMGAIVKDETRCIRCGLCAERCPTGAITMEALEIDESPQTAL
                     GLYHEAMNEVG"
     gene            complement(2828..>3134)
                     /locus_tag="AC16CY_01025"
     CDS             complement(2828..>3134)
                     /locus_tag="AC16CY_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3352384.1"
                     /translation="EKYNLGTWPQGQTKDYTFAKCAKFPCAYTQLCRVHPEMEAYVVV
                     LQNPYFAVTDKAGHYQIEGVPPGDYTVAVWHWKTKAQPTPVTVDAAKPATVDFVLTR"
BASE COUNT          527 a         1074 c         1044 g          489 t
ORIGIN      
        1 gacccagttg aattcgcgcg gcgccttgta ggacccggtc cagaacacgc ggcacatgtg
       61 caggaagacg acgaccacca tggcgtgggc ggaccagcga tgcatgttcc gcagcacgac
      121 gccgaaggtc actacgaact ggaggtcttt catgctgcgg tacgcctcgg gcgggtaggg
      181 cacgtagtag agcatcagcg cgagcccggt caccgacagg accacgaaca ggatcgcggc
      241 gatgaggccg agcccgagcg tcgcggatgg ccgcaggacg tggcggctga cgcgcgccgg
      301 gtggatgtga aggaagatgc tcgagatcat caccgcggcg cggtccttgt ccgtcaccgg
      361 ccagccgatg cgccagatcg accgtccgac ggcgttcagc cacgaggcgg tccgttgtcc
      421 gagcctctga ctcacgtcgg gctcccctca gacccggagc cgaaattccg ggcccaccgt
      481 ctgatccaga tcgacaatca actgctggcg gggcgagagg ttgatggcgt accaggcgag
      541 cggtcgcggc gccggccccg ccacgacgct gccgttctcg tcgaacctgc tgccatggca
      601 cgggcactcg aaccctgccc cggcgtgcct tacgttgcac ccgaggtgcg tgcattccga
      661 cgaaaccgcg aagaacccgt cggcactgtt gaagacgtac aggcgccggt cggcaagaaa
      721 cgtcaccgac ccgggcggaa actccgcggg cgatccgatg tcgaatcgtc gcgacggttc
      781 gtagagcacg ttcggcacca ggaaacggaa agtcgccgcc aggaagccgg cggtggcggc
      841 gccggtgccg aagcagccga cggcgaagat gcgccgccgc tgcagatcaa tctgatcctc
      901 ggtcatcatc ccacctcgtt catcgcttcg tggtacagcc cgagtgcggt ttgcggcgac
      961 tcgtcgatct cgagcgcttc catcgtgatc gcacccgtcg ggcagcgctc ggcgcacagg
     1021 ccgcaccgga tgcagcgcgt ctcatccttc acgatcgcgc ccatctcgtc gctctcgccg
     1081 agggcgtcgc gaacggcatc gagatccggg ctgggccgca actgcgaggc cggcacgagc
     1141 gtcaggcacg cctcggggca gatttcgacg cagccgctgc agagaatgca gagctcggag
     1201 tcgaagatgg tttcgacgtt gcaccacagg cagcgcgacg cctcgaggcg ggcctgctcc
     1261 tcgctgtaac cctcgtccac ttcgcggaag cctgtacgcc gcgcgaccgg aagcgcgggc
     1321 accgcctggc gcgggatccg gtcgtaggga tcgccagcgc ggttcagctc gatcggccgg
     1381 aagcgcgcac gcgcgcgagg caccctgccg ccgccgagcg actcgtgaat cgccagcgcc
     1441 gcccgtttgc cgtcggcctc cgccgaaatc acgatgcggg gcccgaacgc cagatctccg
     1501 ccgcagtaaa cgccggccat cgtcgttgcg agcgtcaccg ggtcgacctt gaccaagccg
     1561 ctcggcatga tttcaatctc cgcgtcggct gcgaggcagg tgggatccgg ctgctggccg
     1621 acagccagga tgatcgtgtc ggccgcgagc cggcgctcgc tccccgggac gaaggtgggg
     1681 ttgaagcgcc cggctgaatc aaacaccgac gcgacatcga gcgtctcgac cccgacggcg
     1741 cggccgttct ctccgagaat cgctttcggc cccagtccgg gcgcgagcgt gatcccttct
     1801 tcctcggctt cgcggacctc atcgggagac gccggcatct ccaggcgcga ctcgagtgag
     1861 acgagctgca cgtggcgtgc gcccaaccgc agggcggtcc gtgccgcgtc gatgaaggcg
     1921 ttgtcgtacc cccaggtttg cagcaactgc tcggcagtga ccggcagctc cggctttccc
     1981 tcggccgccg agtagcgcag cgcggtccgc gcgacgtcaa tggcgacgtt tccgccgccg
     2041 atgacgataa cccgatcgcc gagcgcgacg tggtagccca ggttggcgtt caacagaaag
     2101 tcgacgccgt tgatgacgcc gtcgagatcg gaccccgcga tgttcaactc gcggccgcgc
     2161 gacgtgccga gcgccaggaa gacggcggca tacccgttcc gccgcagatc gctgaccgtg
     2221 aagtcgcgcc cgagcttcca ccccagcttc agttcgacgc caagcgtcag gatccgatcg
     2281 acctcggcgc gaatcagatc gcgcgacagc cggtactcgg gaacgccctg gtacaacatg
     2341 ccgccgacga ccggtgccga gtcgaacagc gtgacgcggt acccgagaag ccccaaatcg
     2401 tgggcgcagc tcaacccggc cggacccgcc ccgacgacgc caatgcgggc cggcccgtcc
     2461 gggcctttga ccgccaggtg cagctgttcg agcggccccg gctgtccgct cagcgtgttt
     2521 tcaaccccgt gacgctccgt cagcgtccgc ttcaaggcgc gaatcgagat cggctgatcg
     2581 atgacgcctc gccggcaatt cgactcgcac gggtgggcgc agatgcggcc gcagatcgac
     2641 gctagcgggt tgggtgcgcg cgcgatgcgg tacccacggg ccgtgtccca ctcggccacg
     2701 gcccgcacgt atccgcccgc attcgtgcgt acgggacagc cggtgcggca tggaatcagg
     2761 ttgaggaagt acctgaggtc cggaacggct gccgtgtacg agcgcaggga catcgctcga
     2821 ccctgcctca gcgcgtgagc acgaagtcga ccgtcgccgg cttggcggca tcgacggtga
     2881 ccggcgtggg ttgtgccttg gttttccagt gccacaccgc taccgtgtag tcgcccggcg
     2941 gcaccccctc gatctggtag tgcccggctt tgtccgtcac cgcgaagtac gggttctgca
     3001 gcaccaccac gtaggcttcc atctcggggt ggacgcgaca gagctgcgtg tatgcgcacg
     3061 ggaacttcgc gcacttggcg aaggtgtagt ccttcgtctg cccttgcggc caggtgccga
     3121 ggttgtactt ctcg
//
LOCUS       DBNGSH010000050         6778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1361525, whole genome shotgun sequence.
ACCESSION   DBNGSH010000050 DBNGSH010000000
VERSION     DBNGSH010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1361525"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="AC16CY_01030"
     CDS             <1..789
                     /locus_tag="AC16CY_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3352385.1"
                     /translation="VRGCLSRLSQVVDEHGGRTVKNMGDGLMCDFPAADKALLAAEAM
                     QVMVTGEEAASQPNLSIRVGCHLGQVVENAGDLFGDTVNIAARIAGVAGAGQIITTQP
                     TVDALSALLKTRVRPLDHVSVKGQADTVAVFDYSWAEHGDATIVRPIARAERSRLRLA
                     RGEHEVWLDASSKPNTIVLGRDPGCGLTVADRAASRQHATVEVRGDKFVLMDHSANGT
                     YVAWGAMETCLKREEMILPAQGQLGLGSSPSAEGVTVLAFSRKS"
     gene            866..1489
                     /locus_tag="AC16CY_01035"
     CDS             866..1489
                     /locus_tag="AC16CY_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HIR3352386.1"
                     /translation="MRSIFTLFIAALCAAATVPAVAQNQPMGFFVSSVGSGMGGNLGG
                     LAGADKHCQALAAKAGAGNRTWQAYLSTSMPDAHARDRIGSGPWYNAKGVLIASNVAD
                     LHSDKANINNDTALDEKGGQINPQGGPNRHDIMTGSTPEGRATTMTCANWTSSASDQT
                     GMLGHHDRLTFGKPGSPWNSAHASKGCSQENLVATGGAGLIYCFAVK"
     gene            1647..2654
                     /locus_tag="AC16CY_01040"
     CDS             1647..2654
                     /locus_tag="AC16CY_01040"
                     /inference="COORDINATES: protein motif:HMM:NF052887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8021 domain-containing protein"
                     /protein_id="HIR3352387.1"
                     /translation="MSRYVWGLALGAVLLGPAGASAQSACDRACLRGLLDQYLSALVK
                     HDPAAAPLVVGFRQTENAINVRPGNGMWKTATGLGKVQRRYLDPVSGQAAYYGTIEES
                     GGTAIVTVRVRVENRQLTEAEWHIARADDPGLSGPRQPGRPPANLHNPDYLARNPPPE
                     RTVPGPQRSDRATLSRIVESYFDAITSHDGSVALTHPGCGRVENGSPAPAGRFLPPAT
                     PANPSAAQPAPAASTNDCVGGLANFNLSMVVARRTPLIDEEAQVALAYAVFIRRPGSP
                     TPRNVFSEWFVIDEAKIRTIYTAMFYPPPELAVPNWPPYEGNWPLPTGIVPPPPQPRQ
                     P"
     gene            complement(2671..5376)
                     /locus_tag="AC16CY_01045"
     CDS             complement(2671..5376)
                     /locus_tag="AC16CY_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3352388.1"
                     /translation="MSPFDSPRSLRAGTPLWRRYLRFWGSDPAADVDDEFAFHLETRV
                     DELVGQGLAPRQAREEALRGFGDIQRVKVICRTLAEERERSMRRIEWWTDWRHDFRFA
                     IRQLAVSPVFTIVTSLTIALGIGATVAIFSVLNAVLLRPLPWAGSDRIVIVYETWKQF
                     RQGNASVGHFHDWTEQNEVFEHTAALTRATFNLSDGEPERVSGTRITPGFFDVMQLPP
                     VLGRYFTAQDVERDARLVVLGHGLWQRRFAGDPATVGRTVRLNGQAHTVVGIAPPESN
                     MNRFAPQILAPLVFSPEQRANYGSHSFTVLAKLKAGVTREAAQDDMERVTRGIAERQP
                     RAMEGRSVSVRSYTDILLGNFRTPLYVLLASVVFVLLIGCVNVANLLLARATTRRREI
                     AIRAAIGGGRWRIVRQLLTESIVLSVVGGTAGIGLAYVGIRSLVSFGPPNVPRLQNAG
                     LQPEVLVFAAGITLLTGFVFGLAPAFRAARQDLLPTLREGTKGSIAAAGRDRLRGALV
                     VGEIAVAVVLLVGAGLFIRSAWRLQQVPLGFETRGALSARLALPIDRYRGPEAVADAY
                     RRILEEARAVPGIRHAGASTGIPLVGGGPDASVQIEGKPYTPGSAISPAIRLITDGYV
                     EAIGMSVVRGRTMRPADMAPGAPPVVLINARLASAAWPGEDPIGKHLSTWTREIDTPE
                     WREVVGIVGDVRSFGPDTPPPPELFLPYTQPPVGAWAAFQGSMAIVVRAADDPASYAP
                     ALRRSVRSVDASLPLFDVQTMDQALGADAAGVRFNTVLLSLLAAVGLVLAAIGIYGVI
                     AYFVTQRTPEIGIRLALGASPGSVLMMVLRRGTVLALTGIAIGLIAARDLTRLVTTLL
                     FEVTPTDPPTYAAGAIALLTIALLACVVPALRAVRVNPMRSLADS"
     gene            complement(5373..5711)
                     /locus_tag="AC16CY_01050"
     CDS             complement(5373..5711)
                     /locus_tag="AC16CY_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3352389.1"
                     /translation="MEKELLHGTLEVLILKTLSWGPMHGFGIGRWIEQLSGNELRIEE
                     GSLYPALYRIEKKGWVDASWGVSDNQRRAKFYRLTTEGRRQLLRNADTWSNFAHAVAR
                     VLGAKQQPAS"
     gene            5857..>6778
                     /locus_tag="AC16CY_01055"
     CDS             5857..>6778
                     /locus_tag="AC16CY_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352390.1"
                     /translation="MVRVVYVSTLIGIACLTLAAAGDQRDGPSGSGYRVPRTSFGQPD
                     LEGVWTNNSATPLQRPAAWADKQSLTDAEVEQVQKVARELEKEGDALFGDELITDTLQ
                     GKTAPKSHDTETGNYNGFWLPARDFERRTSLITDPPDGRIPPETPAAQARRAALAEQR
                     RLHPADGPESRGLTERCITFGVPRFGAAYSSVYQIVQSPAHVVFNMETIHDARVIPID
                     GRPHLSERIPQWLGDSRGHWEADTLVVDTSGFSPKSNMLGSTSGLRLTERFTRVAADT
                     LQYDATLSDANTWTRPWTARIYFKRTDEPLL"
BASE COUNT         1214 a         2289 c         2272 g         1003 t
ORIGIN      
        1 gtgcgcggct gcctgagtcg gctgagccag gtggtggacg agcatggcgg caggacggtc
       61 aagaacatgg gcgacggcct catgtgtgat ttcccggccg ccgacaaggc gctgctggcc
      121 gcggaggcga tgcaggtgat ggtgacaggc gaagaggccg cctcccagcc gaacctgagc
      181 attcgcgtcg gatgccacct gggccaggtc gtcgaaaacg ccggagatct gttcggcgac
      241 acggtcaaca tcgcggcgcg tatcgcgggc gtcgccggcg ccggacaaat catcacgacc
      301 cagccgacgg tcgatgcgct cagcgccctg ctgaagacga gggtccggcc actggaccac
      361 gtgagcgtca agggccaggc cgacaccgtg gcggtgttcg actactcgtg ggcggagcac
      421 ggagacgcca ccatcgtgcg gcccatcgcc agggccgagc gctcgcgcct caggctggcc
      481 cgcggcgagc acgaggtgtg gctcgacgcc tcgtccaagc cgaacacgat cgtgctgggc
      541 cgcgacccgg ggtgcgggtt gaccgtcgcc gaccgggcag cctcccggca gcacgcgact
      601 gtcgaggtcc gcggcgacaa gttcgtcctg atggaccaca gcgccaacgg cacctacgtc
      661 gcgtggggcg cgatggaaac gtgcctgaag cgcgaagaga tgattctgcc tgcccaaggc
      721 cagcttggac tcgggtcgtc tccctcggcg gaaggagtca ccgtcctcgc cttctctcgc
      781 aaatcctgac ccccgccccg cgcgcccggt tccacatcga ttcccgatgg acgtagtatt
      841 gagtcccctg agaggaggga gatcgatgcg gagtatcttc acgctcttca tagcggcgct
      901 gtgcgcggcg gccacggtgc cggcagtggc ccagaaccag cccatgggct ttttcgtgag
      961 cagcgtcgga tcgggcatgg gcggcaatct cggcggcctc gccggcgcgg acaagcactg
     1021 ccaggcgctc gccgccaagg ccggcgcggg caatcgcacg tggcaggcgt atctcagcac
     1081 cagcatgccc gacgcccacg cccgcgaccg gatcggatcg ggcccgtggt acaacgcgaa
     1141 gggagtgctc atcgcgtcca acgtggccga tcttcactcg gacaaggcga acatcaacaa
     1201 cgacacggcg ctcgacgaga agggtggtca gatcaacccg caaggagggc ccaatcgcca
     1261 cgacatcatg accggctcga cgcccgaagg ccgggcaacc accatgacct gtgcgaattg
     1321 gacgagcagc gcctccgatc agaccggaat gctggggcat cacgaccgtc tcacgttcgg
     1381 caagcccgga tcaccgtgga attccgccca cgcgtccaag ggctgctcgc aggagaacct
     1441 ggtggccacc ggcggagcgg ggttgattta ctgcttcgcg gtgaagtgac ctttggtcct
     1501 tggtgcttgg tgcttggtcc ttagtccgtc gcttggttcc tggtccgtcg cggaccaagg
     1561 accacggacc aaggacacca agaacaacgg acggaccaag gacgaagacc taaggaccaa
     1621 ggaccaagga tcgacaggag agaacgatgt cacgatacgt gtggggtctg gctcttggtg
     1681 cagtactgct ggggccggcc ggcgcctccg cgcaatccgc gtgcgatcga gcctgtctgc
     1741 gcggcctgct cgatcaatac ctgtccgccc tcgtcaagca cgatccggcc gccgccccgc
     1801 tcgtcgtcgg cttcaggcag acggagaacg cgatcaacgt ccggcctggc aacggcatgt
     1861 ggaagacggc cacggggctt ggcaaggtac agcggcgcta cctcgatccc gtgtccggcc
     1921 aggcggcgta ctacgggacg atcgaggaaa gcggcgggac ggcaatcgtg acggtgcgcg
     1981 tgcgtgtcga gaatcgacaa ctcaccgagg ccgaatggca catcgcccga gccgacgatc
     2041 ccgggctcag cgggccacgg cagcccggcc gaccgccggc caacctccac aacccggact
     2101 acctggcgag gaaccctccg cccgagcgta ccgttcccgg ccctcagcga tccgatcgcg
     2161 cgacgctctc gcgcatcgtc gagagttact tcgacgcgat cacgtcgcac gacggttccg
     2221 tcgcgctgac gcatcccggc tgcggccggg tcgagaacgg ctcgccggcg cccgccggcc
     2281 gcttcctgcc gccggccacg cccgccaacc cgtcagcggc gcagcccgca cctgcggcat
     2341 cgacgaacga ctgcgttgga ggcctcgcga acttcaatct ctcgatggtg gttgcgcggc
     2401 ggacgccgct gattgacgag gaggcgcagg tcgcactcgc ctacgcggtg ttcatccgcc
     2461 gtccgggctc gccgacgccg cggaacgtgt tcagcgagtg gttcgtgatc gacgaagcga
     2521 agatcaggac gatttataca gcaatgttct acccgccgcc cgagctcgcg gttccgaact
     2581 ggcctcccta cgagggcaac tggccgctgc cgacgggcat cgtccccccg ccgccgcagc
     2641 cgcggcagcc gtgacacgag gcgccgccgc tcaggaatcc gccagcgagc gcatcgggtt
     2701 cacgcgaacc gcccgcagcg ccgggaccac gcacgccagc aacgcgatcg tcagcagtgc
     2761 gatcgctccg gccgcgtagg tcggcggatc ggtcggcgtg acctcgaaca acagcgtcgt
     2821 gaccagccgg gtgagatccc gcgccgcaat caacccgatt gcgatgcccg tgagggcgag
     2881 gacggtcccg cggcgcagga ccatcatcag caccgaaccg ggtgacgcgc caagcgccag
     2941 ccgtatgccg atctcgggcg tccgctgcgt gacgaagtag gcaatcacgc cgtagatgcc
     3001 gatcgcggcg agcacgagac caacggcagc cagcagcgac agcagtacgg tgttgaatcg
     3061 gacgcccgcc gcatcagcgc cgagggcctg gtccatcgtc tgcacgtcga agaggggaag
     3121 cgacgcgtct acggaccgca ccgagcgtcg cagcgcggga gcgtacgacg cagggtcgtc
     3181 agcagcacga acgacaatcg ccatgctgcc ctggaatgcg gcccaggccc cgacaggggg
     3241 ctgcgtgtac ggcaggaaca gctccggtgg cggcggcgta tcgggaccga aactgcgcac
     3301 gtcgccgacg atgccgacga cttcccgcca ttcgggcgtg tcgatctcgc gagtccacgt
     3361 cgagagatgc ttgccgatcg gatcctcgcc cggccacgcc gcgctcgcga gtcgcgcgtt
     3421 gatgaggacg actggcggcg ccccaggcgc catgtcggca ggccgcatcg tccggccgcg
     3481 gacaaccgac atgccgatcg cttcgacgta gccgtcggtg atcaggcgga tggccgggct
     3541 gatggcgctc ccgggcgtat atggcttgcc ttcgatctgc acgcttgcat cgggaccacc
     3601 gccgaccagc gggattccgg tcgatgcgcc ggcatgccgg atccccggaa cggcccgcgc
     3661 ctcttcgaga atccgacggt acgcgtccgc caccgcctcc gggccgcgat atcgatcgat
     3721 cggcagcgcc aggcgggcgc tgagcgcgcc gcgcgtctcg aagccgaggg gtacctgctg
     3781 caatcgccac gcgctgcgaa tgaagaggcc ggcgccgaca aggagcacga cggcgacggc
     3841 aatctcgccc acgacgagcg cgccccgaag ccggtcgcgt cccgcggcgg caatggagcc
     3901 cttcgtgccc tcgcggagcg tcggcagcag gtcctgacgg gccgcgcgaa acgccggcgc
     3961 gaggccgaag acgaaaccgg tcaacagcgt gatgccggcc gcgaagacga gcacttcggg
     4021 ttggaggccc gcattctgga gccgcgggac gttgggcggg ccgaagctca caagcgatcg
     4081 aatgccgacg tacgcgaggc cgatgcccgc ggtgccgccc acgaccgaca gcacgatgct
     4141 ctctgtgagc aattggcgca cgatgcgcca gcggcctccg ccgatggcgg cgcgaatcgc
     4201 gatctcgcgc cggcgggtcg tggcgcgcgc cagcagcaga ttggcgacgt tgacgcatcc
     4261 gatcagcagt acgaagacga ccgacgcgag cagcacgtac aacggcgtgc ggaagttgcc
     4321 cagcaggatg tcggtgtacg atcgcacgct gacggatcgc ccctccatgg cccgcggctg
     4381 acgctcggcg atgccgcgag tgacgcgctc catgtcgtcc tgcgcggcct ctcgcgtcac
     4441 tcccgctttc aacttcgcga gcacggtgaa cgaatgcgat ccgtagttcg cccgctgctc
     4501 cggcgaaaag acgagcggcg ccaggatctg cggggcgaag cggttcatgt tgctctcggg
     4561 cggcgcgatg ccgacgacgg tgtgggcctg cccgttcagc cgaacggttc gaccgacggt
     4621 ggccgggtcg ccggcgaaac gccgctgcca gagcccgtgg ccaagcacca cgagacgcgc
     4681 gtcgcgctcc acatcctggg cggtgaagta ccggccaagc acgggcggca actgcatcac
     4741 gtcgaagaac cccggcgtga tacgcgtccc gctcacacgc tcgggctcgc cgtccgacag
     4801 gttgaaggtg gcgcgtgtca gcgccgccgt gtgctcgaac acctcgttct gttcggtcca
     4861 gtcgtggaaa tggccgaccg acgcgtttcc ctgacggaac tgcttccagg tctcgtagac
     4921 gatgacgatg cgatcgctgc cggcccaggg gagcgggcga aggaggacgg cattgagcac
     4981 gctgaagatc gccaccgtcg cgccgatgcc gagtgcgatg gtcagtgagg tcacgatcgt
     5041 gaagacgggg ctcacggcca gttgacggat ggcgaaccgg aagtcgtggc gccagtcggt
     5101 ccaccactcg atgcggcgca tggatcgctc tcgttcttcc gccagcgtcc ggcagatgac
     5161 tttcacccgc tggatgtcgc caaagccgcg cagcgcctcc tcgcgcgcct gccggggtgc
     5221 aagcccctgc cctacgagct cgtcgacgcg cgtctcgagg tggaaggcga actcgtcgtc
     5281 aacgtcggcc gccgggtcgg aaccccagaa gcgcaggtag cgccgccaca ggggcgtgcc
     5341 agccctgagc gagcgaggcg agtcgaacgg gctcatgacg ccggctgctg tttggcgccc
     5401 agcacgcgtg ccaccgcgtg ggcgaagttc gaccaggtgt ccgcgttcct gagcagctgc
     5461 cggcgccctt ccgtcgtgag gcggtagaac ttggcgcggc gctgattgtc ggacacgccc
     5521 cacgacgcat cgacccaccc cttcttctcg atgcggtaga gcgccgggta gagcgagccc
     5581 tcctcgatgc ggagctcgtt cccgctcaac tgctcgatcc agcggccgat gccgaagccg
     5641 tgcatgggtc cccagctgag cgtcttaagg atgaggactt cgagcgtgcc gtgcaggagt
     5701 tccttttcca tggtcccacc ctagataatc tagggcgaac atactctgac ttccctagat
     5761 agtcaaggga gcgtcctggc ggcgcctcgg cggccgtaaa ctacgcatat cgttcactgc
     5821 cgtctgtgga ccgcacggat gaaaaggagc atgacgatgg ttcgcgtagt gtacgtcagc
     5881 acgttgatcg gaatcgcgtg cctgactctt gcggccgccg gggatcagag agatggaccg
     5941 tcggggtcgg gataccgggt gccaagaacc tcgttcggcc aacccgatct cgaaggcgtg
     6001 tggaccaaca acagcgccac gcctctgcag cgtcctgccg cctgggcaga caagcagtct
     6061 ctgaccgacg ctgaggtcga acaggttcag aaggtcgctc gagagctcga aaaggagggc
     6121 gacgcgctct tcggtgacga actgatcacc gacacgcttc aggggaagac ggcgccgaag
     6181 tcgcacgaca cagagacggg aaactacaac ggcttctggc tgcccgcgcg cgacttcgaa
     6241 cggcgcacct cgctcatcac cgatccgccc gacggacgaa tccctccaga gactccggcg
     6301 gcccaggccc ggcgagccgc cctggccgaa caacgacggc tgcatccggc cgacggccct
     6361 gaaagccgcg ggctgaccga gcgctgcatc accttcggcg tgccgcgctt cggggcggcc
     6421 tatagcagcg tctaccagat cgtgcagtct cccgcgcatg tagttttcaa tatggagacc
     6481 attcacgatg cgcgagtgat cccgatcgac ggacgtccgc atttgtccga gcgaatcccg
     6541 caatggcttg gcgactctcg cggacactgg gaggctgaca cgctcgtcgt ggacacgtca
     6601 ggcttctcac ccaagagcaa tatgctggga tccacgagcg gactgcgcct caccgagcgg
     6661 ttcacccgtg tcgcggcgga cacgcttcag tacgacgcca cgttgagtga tgcgaacacg
     6721 tggacgagac cttggaccgc gcggatctac ttcaagcgta cggacgagcc gctcctgg
//
LOCUS       DBNGSH010000051         2824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2889920, whole genome shotgun sequence.
ACCESSION   DBNGSH010000051 DBNGSH010000000
VERSION     DBNGSH010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2889920"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1238
                     /locus_tag="AC16CY_01060"
     CDS             141..1238
                     /locus_tag="AC16CY_01060"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3352391.1"
                     /translation="MSLRWALLLGAGGASLIALTVSGQIYLSMLTHGHSFLRIAAWQL
                     TSWSVWAAATPLVLRLGARLSDRSSPLRNRVLLVIVTGLGIVTVHVLIGSLSTVWFQP
                     YVPVQTFRLGTALILQRLSLPVDLLVFGLILLTGSAIAVYNRARILELRESRLEADLA
                     RSQLDALRLEIEPHFLFNTLNSIGSLIRARSGDRALSMLLGLSELLRATIDGTRDHTT
                     TLGAEAAFVKRYIELQRARFSDRLEVTYDLSPESEACAVPSFLLQPVVENAFRHGLAK
                     RSGRCRLELAASVEGADLHVQVRDDGAGLPNDFNLTRHAGTGLRNTRQRLERLYDQAA
                     QLTLEPADGGGTVVHIRLPAQAGVQEQRTLR"
     gene            1235..2026
                     /locus_tag="AC16CY_01065"
     CDS             1235..2026
                     /locus_tag="AC16CY_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3352392.1"
                     /translation="MTKFKVLVVDDEPLARQVAVNLLRRDPEVEDVAECGDGLRAKQT
                     IAELRPDIVFLDIEMPGLSGVQLAEDLHGGSPVVVFMTAFSQYAVDAFGIAATDYVLK
                     PFSDERFLEALGRAKRRVRERHLGELAQQMAGVAAELQPAEAGGPVSPGVRYLQRLSI
                     RHGERTLVLRTEEIVWIEAQDYCVMVYSTRGKHLVRASLASLEERLAPDEFVRAHRMA
                     IVNLRHVRETDDRDGLRLLLSDGSHVSVSRSRKAQVEALLSPRLR"
     gene            2146..2757
                     /locus_tag="AC16CY_01070"
     CDS             2146..2757
                     /locus_tag="AC16CY_01070"
                     /inference="COORDINATES: protein motif:HMM:NF028004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3352393.1"
                     /translation="MPSRSQTGHRLILASALFAWIAVAAAGNFAVAEQSPAAVPSSGT
                     MPRYDSNRNLTLPSDYRQWILVGSSLGLSYAEGALDHEMFVSTLMEPSAYRHFTETGT
                     FREGTMLALVLQGVGTNAAPARQGQFATDVHVIEMAVKDSSRVAEGWAYYGFGGPMAG
                     GYRSSAAPEPKDRCYDCHAQHAARDNVFTQFYGLLNEAAPRAK"
BASE COUNT          426 a          948 c          956 g          494 t
ORIGIN      
        1 gcgtgacgcc ggccgccgcc ggatcgcggc ccttcgctgc aacccgtttg ccccggccgt
       61 cacggaccgc tatcctgcgg tccggacggc tcatgcatcc agtcgagcgt cgtgcgatct
      121 ccgacgcgag ttcttctgtc ttgtccctcc ggtgggcgct gctgctcggg gcaggtggcg
      181 ccagcctcat cgcgttgacg gtgtcggggc agatctacct ctcgatgctg acgcacgggc
      241 actcgtttct ccggatcgcc gcgtggcagc tcacctcgtg gagcgtctgg gcggccgcaa
      301 cgccgttggt cctgcggctc ggcgcgcggc tgagcgatcg gtcgtcacca ctccggaacc
      361 gcgtgctcct ggtgatcgtc acgggcctcg gaatcgtcac cgtgcacgta ctgatcgggt
      421 cgttgtccac cgtctggttc cagccgtacg tgcccgtgca gacattccgg ctcgggaccg
      481 cgctgatcct gcagcggctc tcgctgccgg tggacctgct ggtcttcggc ctcatactcc
      541 tcaccggatc ggcgatcgcg gtctataacc gcgcgcggat tctcgagctg cgcgagtcgc
      601 gcctcgaggc cgatctcgca cgctcccagc tcgatgcgtt gcgtctcgag atcgagccgc
      661 acttcctgtt caacacgctg aactcgatcg gctcgctcat ccgcgcgcgc tccggcgacc
      721 gcgccctttc gatgctgctc gggctgagcg agctgctccg ggccaccatt gacggcacgc
      781 gcgaccacac gaccacactc ggcgccgagg ctgctttcgt aaagcgatac atcgagctgc
      841 agcgcgcgcg tttcagcgat cggctggagg tgacctacga cctttcgccg gaatccgagg
      901 cgtgcgccgt gccgtcgttc ctgctgcagc cggtggtgga gaacgccttt cggcacggcc
      961 tggcgaagcg gtcgggtcga tgccgcctcg aactggccgc gtcagtcgag ggcgccgacc
     1021 tccacgtgca ggtccgcgac gacggcgcgg gactgccgaa cgacttcaac ctgacccggc
     1081 acgccggcac ggggctgcgg aacacgcggc agcggctcga gcgtttgtac gaccaggccg
     1141 cgcagctgac gctcgagccc gccgacggcg gcggaaccgt cgtccacatc cggcttcctg
     1201 cccaggcagg cgtgcaggag caacggacgc ttcgatgacc aagttcaagg tgctcgtggt
     1261 ggacgacgag ccgctggccc gccaggtagc cgtgaatctg ctgcgtcgcg atccggaagt
     1321 ggaggacgtg gccgaatgcg gcgacggcct gcgcgcgaag cagacgatcg ccgagctgcg
     1381 gccggacatc gtgttcctgg atatcgagat gcccggcctg agcggcgtgc agctcgccga
     1441 agatctgcac ggcggatcgc cggtcgtcgt cttcatgacg gcgttcagcc agtatgcggt
     1501 ggacgcattc ggcatcgccg cgaccgacta cgtcctgaag ccattctccg acgagcggtt
     1561 tctcgaggcg ctcggcagag cgaagcggcg ggtgcgcgag cggcacctgg gagagcttgc
     1621 gcagcagatg gctggcgtgg ccgccgagct tcagccagcc gaggcgggcg ggccggtgtc
     1681 gccaggcgtc cggtacctgc aacggctctc gatcaggcat ggcgagcgga cgctcgtgct
     1741 ccggacggag gaaatcgtct ggatcgaagc gcaggactat tgcgtgatgg tgtattcgac
     1801 cagggggaaa cacctggtcc gggcctcgct ggcctcgctc gaagagcggc tggctccgga
     1861 cgagttcgtc cgcgctcatc ggatggccat cgtgaacctg cgccacgtgc gggagaccga
     1921 cgatcgcgac ggccttcggc tgctgctgtc ggacgggtcg cacgtgagcg tgagccgctc
     1981 ccgcaaggcg caggtcgagg cgctcctctc gccccggttg cgctgacccg tgaatgcagc
     2041 gaacggtcga tccacggccg tgaaacgtga aggctgcatg tcgctgcaaa gatgttggat
     2101 cgccggtcta cgtcgaatga tcatcgttgc aggaggtcat tgcacatgcc aagccgttcg
     2161 caaacaggcc atcgtctgat cctcgcgagt gccttgttcg cgtggattgc cgtcgcggcg
     2221 gccggaaact tcgcggtcgc ggagcaatca ccggccgcag taccgtcatc gggtacgatg
     2281 ccgcgctacg actcgaacag gaacctcacc ctgccgagcg actaccggca gtggatcctc
     2341 gtcggctcgt ccctcggcct gagctacgcc gagggcgcac tggaccatga gatgttcgtc
     2401 tcgacgctga tggagcccag cgcctaccgt catttcacgg agaccggtac gttccgtgaa
     2461 ggcacgatgc tggcgctcgt ccttcagggg gttggcacca acgccgctcc ggcccgtcag
     2521 gggcagtttg ccaccgacgt gcatgttatc gagatggccg tcaaagacag ctcgcgagtc
     2581 gcagagggtt gggcgtacta cggctttggc gggcccatgg cgggcggata ccggtcctcc
     2641 gcggcgccgg agccgaagga ccgctgctac gactgtcatg cccagcacgc cgcacgcgac
     2701 aacgtcttca cgcagttcta cggccttctg aacgaagcgg ccccgcgggc gaagtaggtc
     2761 gcacggcgtg cgccggctcg tcgagcgcca tccgatcgtc tcgtcgctgg ccggcctggc
     2821 cgtc
//
LOCUS       DBNGSH010000052         7604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_342754, whole genome shotgun sequence.
ACCESSION   DBNGSH010000052 DBNGSH010000000
VERSION     DBNGSH010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_342754"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2304
                     /locus_tag="AC16CY_01075"
     CDS             <1..2304
                     /locus_tag="AC16CY_01075"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR3352394.1"
                     /translation="GVDVAAVFEDGHIIIPSHATRAGINRIEFEFTAGDDALNRNDEF
                     LYTLFVPARARLAFPCFDQPDLKARYTLRLSVPDGWQAVANGASRAGGAGGAGGVSAS
                     PGSSVFEFVETAPLPTYLFAFAAGKFSVETATRNGRTFRMFHRETDAAKVARNREAVF
                     DLHASALAWLESYTAIPYPWGKFDFVLIPSFQFGGMEHAGAILYNASGLLLDESATQN
                     QYLARATTIAHETAHMWFGDLVTMRWFNDVWTKEVFANFMAGKIVNPSFPDVNHELRF
                     FLEHYPSAYQVDRTAGTNPIRQDLANLDQAGQMYGPIIYEKAPIVMRQLEMIVGERAF
                     QDGMREYLKRFSFGNATWDDLIRILDARTRENLARWSRAWVEERGRPRFTTDVRVGST
                     GDLERIELTLEDPLDRGLVWPERLRVAMGYPGGVREVGASVTSRRTTVRVPRGLGRPQ
                     FVLPNGGGLGYGLFVLDEASREYLLGHIEEVPDALTRGAAWVTLWDNVLEARVGAPVF
                     LDAAMRALPRESDEQNAQRVLAYVTRVFWRHLTPDERRARSAPLESMLRSGIERVPTQ
                     SRKAAWFNAYRDTVVSAEGLAWLERVWRREESIPGLTLAEPDEITLALELAVREVPGA
                     DQILRAQADRTQNPDRKARFEFVTPALSGDPAVRERAFARLRFVENRRREPWVLESLQ
                     YLNHPLREAHARRFVLPALELLREIQQTGDIFFPSRWTEATLGGHRSPEAAGIVRDFL
                     AGNPEYPQRLRWTILTAADELFRVAER"
     gene            complement(2386..3216)
                     /locus_tag="AC16CY_01080"
     CDS             complement(2386..3216)
                     /locus_tag="AC16CY_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3352395.1"
                     /translation="MIKVIAIPDKANAPSPEGRVTHVLNPADDGTRVAVAVREVEPGK
                     TYRVARGDKTQVAYILEGTDATLTLSSAGGTTEHRTGRRAGVYVEPGDEAAVTASATT
                     LTLLLVTVPKHTGRSTGEGTPASYVFEESRLRSLVDEKRFRERTFWVNKETGLSGAWD
                     LQLGRMQYAPHAHSPRHVHHASKTSTITPEHFYLIEKGTGEVKHDTGSLPVGPGCLVL
                     IPAGEWHQLIASDSGFDYIEFQAPFDFVTTMDHDPLGKNWYIKGTDDGTGKPKLWVQS
                     "
     gene            complement(3226..4596)
                     /gene="ligM"
                     /locus_tag="AC16CY_01085"
     CDS             complement(3226..4596)
                     /gene="ligM"
                     /locus_tag="AC16CY_01085"
                     /EC_number="2.1.1.341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046274 - lignin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanillate/3-O-methylgallate O-demethylase"
                     /protein_id="HIR3352396.1"
                     /translation="MTQSLEDKLRTAGSPVEMLRNSQIGPYAFPVVAPEYTNWRDEQR
                     AWRESCVLFDQSHHMTDLYVEGRDVLKLLSDLGVNSFTTFKVDQAKQFIACNHEGYVI
                     GDAILFYLGDNRLSLVGRPSALNWVQFNVETGGYKVTAERDERSAMNRGTRRSFRYQV
                     QGPHAVRVMEKVTGKPTPDIRFFNMGVFAIAGHDVRALRHGMVGQPGWEMWGPWEHAE
                     AVRDAILEAGREFGIRRVGSRAYPTTCLESGWIPSPVPAVYVGERMKGYRQWLGPTSY
                     EAMASLGGSFYSDDIRDYYLTPYDLGYGPFVKFDHNFIGREALERMAQKRKKVTLVWN
                     GDDVARVFRSLSDSGDAAKYIDLPLANYATLPFDKVMKAGKTVGVSTYTGYTYNERAI
                     ISLAVVDNEHAEPGTDVTLLWGEEGRGSSKPTVERHVQVEIRATVQPAPISEVARVAY
                     RPKVMA"
     gene            complement(4649..5410)
                     /locus_tag="AC16CY_01090"
     CDS             complement(4649..5410)
                     /locus_tag="AC16CY_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR3352397.1"
                     /translation="MEGLPISPALVFLVLLVLYALSSIQILNEYERAVVFRLGKLLPS
                     PKGPGIVLVFRPIDRYVRMSLRTVVLDVPPQDIITRDNVSLKVNAVVYFRVIDPSKAV
                     VEVENYMYATSQLSQTTLRSVLGQVELDDLLAERERLNQQLQHILDNQTDPWGIKVSA
                     VEVKHVDLPQDMQRAIARQAEAEREKRAKIIHAEGELTASDKLAQAAAIIDRQPAAMT
                     LRYLQTLTEIAAEQNSTIIFPLPIELMRMLRVDTK"
     gene            complement(5410..6744)
                     /locus_tag="AC16CY_01095"
     CDS             complement(5410..6744)
                     /locus_tag="AC16CY_01095"
                     /inference="COORDINATES: protein motif:HMM:NF051235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3352398.1"
                     /translation="MPRLRLLVAAVLLCCASGRVQAQEPAPASRQVIVTEIDGIIHPV
                     TAEYFTSAVDVADTSGAELVVLVLRTPGGLLDSTRTMVSRMITSRAPVVVFVGPSGER
                     AASAGFILLLAADVAVMAPGTHAGAAHPVSGTNEKMDETTSKKAAADAAAYARSLAEA
                     RGRNVALAAEAVLESRAFTDREALDVKPPLISLIAKDVDEILAQLDGRAITRFDGRST
                     TVHTKGAEIRRIEMTRRQWFLSAIAHPQIAYMLMTLGLLGLTVEIWNPGLIAPGVIGG
                     VCLLLAFFAFQILPVSTAGVLLVIFGLALLVLELKVPSFGALGVGGTVSLLIGSVMLT
                     DVVPGVRVGLGIIVPAALSVSALFLFLGRLALKAQRQPAATGASALVGQTARVRTMLA
                     PGEPGLVDVHGEIWRAVSLVAVPAGHPVRVTRVDGLTLTVEPAEAAQRKGDD"
     gene            6851..7300
                     /gene="mscL"
                     /locus_tag="AC16CY_01100"
     CDS             6851..7300
                     /gene="mscL"
                     /locus_tag="AC16CY_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR3352399.1"
                     /translation="MLKEFRDFVARGNVLDLAVGVIVGAAFGRVVSSLVQGVLMPPLG
                     LLLGRTDFSGLFLVLDRSKDLPATLKEATDKGVPVIAYGQFINEIVSFLIVALAVFLL
                     VRQVNQLKRRLEGPPPPAAPPATKDCPFCVSAIAIRATRCPHCTSQL"
     gene            complement(7302..>7604)
                     /locus_tag="AC16CY_01105"
     CDS             complement(7302..>7604)
                     /locus_tag="AC16CY_01105"
                     /inference="COORDINATES: protein motif:HMM:NF046915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HIR3352400.1"
                     /translation="QVQAQVLGHGIYNVRAQYGFMQDPDVPDALLKAREQGVELDLDD
                     LTYFLGRETIIITRRPGMAVWRERLFVVMARNAVRATAFFRLPTERVVELGVQVEM"
BASE COUNT         1191 a         2562 c         2527 g         1324 t
ORIGIN      
        1 ggcgtggacg tcgccgccgt tttcgaggac ggccacatca tcatcccgtc gcatgcgacc
       61 cgcgccggga tcaaccgcat cgaattcgag ttcaccgccg gcgacgatgc cctcaacagg
      121 aacgacgagt ttctgtacac gctgttcgtg ccggctcgtg cgcgcctggc gtttccctgc
      181 ttcgaccagc cggacctgaa ggcgcgctac acgctgaggc tgtccgtacc ggacggatgg
      241 caagccgtgg ccaatggggc gagtagggcg ggtggggcag gcggggcggg tggggtgagt
      301 gcgagtccgg ggtcctccgt gttcgagttc gtcgagacgg caccgctccc gacgtacctc
      361 ttcgcttttg ccgcgggcaa attctccgtc gaaacggcga cgcggaacgg acgcacgttc
      421 cgcatgttcc accgagagac cgacgccgcc aaggtggcgc ggaaccgaga ggccgtcttc
      481 gatctgcatg cgtccgcgct cgcgtggctc gagtcgtaca cggccattcc gtatccctgg
      541 ggcaagttcg atttcgtcct gattccgtcg ttccagttcg gcggcatgga gcacgcgggc
      601 gcgatcctct acaacgcgtc cgggctgctg ctcgacgagt cagctacgca gaaccagtac
      661 ctggctcgtg ccaccaccat cgcgcacgag accgcccaca tgtggttcgg cgacctcgtc
      721 acgatgcggt ggttcaacga cgtgtggacc aaggaggtct tcgccaactt catggcggga
      781 aagatcgtca acccgtcgtt cccggacgtg aaccacgagc tgcgcttctt cctcgagcac
      841 tacccgtcgg cctatcaggt ggaccgtacg gccggcacca atcccatccg ccaggatctg
      901 gcgaacctcg atcaggcggg ccagatgtac ggcccgatca tctacgagaa ggcgccgatc
      961 gtcatgcgcc agctcgagat gatcgtcggc gagcgcgcgt tccaggacgg catgcgcgag
     1021 tacctgaagc gcttctcgtt cggcaacgcc acgtgggacg acctgattcg gattctcgat
     1081 gcgcggactc gggagaacct ggcgcgctgg agccgcgcct gggtggagga gcgcggtcgt
     1141 ccgcgtttca ccaccgacgt gcgcgtcggc tcgaccggag atctcgagcg catcgaactg
     1201 acactcgagg acccgctcga tcgcggcctg gtctggccgg agcggctccg tgtcgcgatg
     1261 ggctaccccg gtggggtccg tgaggtcggg gcgtccgtca cgagccgtcg cacgaccgtc
     1321 cgcgttcctc gcggacttgg ccgcccgcaa ttcgtgctgc cgaacggcgg cgggctcggc
     1381 tacggcctgt tcgtgctgga cgaggccagc cgcgagtacc tgctgggtca catcgaggag
     1441 gtgcccgacg cgctcacgcg cggcgcagcg tgggtcacgc tgtgggacaa cgttctcgag
     1501 gcgcgcgtcg gcgccccagt ctttctcgat gcggccatgc gcgcgctgcc gcgcgagagc
     1561 gacgagcaga acgcacagcg cgtgctcgcc tatgtgacgc gcgtgttctg gcgccatctg
     1621 acgccggacg agcggcgggc gcgcagcgcg ccgctcgagt cgatgctgcg gtcgggcatc
     1681 gagcgcgtgc cgacgcagag ccgcaaggcc gcctggttca acgcgtaccg cgacaccgtc
     1741 gtgtcggcgg aaggactggc atggctcgag cgcgtgtggc gccgcgagga atcgattccg
     1801 ggcttgacac tcgccgaacc ggacgagatc acgctcgcgc tggagctcgc cgtgcgcgag
     1861 gtgccgggcg cggatcagat cctgcgggcg caggcagacc ggacgcagaa cccggatcgg
     1921 aaggcgcgct tcgagttcgt cacgccggcg ctctcaggcg accccgccgt ccgcgagcgc
     1981 gcgttcgcac gcctgcgctt cgtggagaac cgccggcgtg agccctgggt gctcgagtcg
     2041 ctccagtacc tcaaccaccc gctccgcgag gcgcacgccc ggcgcttcgt gctgcccgcc
     2101 ctcgagttgc tgcgcgagat ccagcagacc ggggacatct tcttcccgtc gcgctggacc
     2161 gaggccacgc tgggaggaca tcgctcgccc gaggcggcgg gcatcgtgcg ggacttcctg
     2221 gccgggaatc ctgaataccc gcagcggctc agatggacga tcctgacggc ggccgacgag
     2281 ctgtttcgag tggcggagcg gtgaaaatgg ggtcgggagt catttttcga ggacaccccc
     2341 tgggccacaa ttgctgtgtc gccaaaaaat gactcccgac cccatttaac tctggaccca
     2401 cagctttggc ttgcccgtcc cgtcgtcggt ccccttgatg taccagttct tgcccagcgg
     2461 gtcgtgatcc atcgtggtca cgaagtcgaa gggcgcctgg aactcgatgt agtcgaatcc
     2521 gctgtcggac gcgatcagct gatgccactc gccagccgga atcaggacga ggcagccggg
     2581 gccgacgggg aggctcccgg tgtcgtgctt cacttcgccg gtccctttct cgatcagata
     2641 gaagtgctcc ggcgtgatcg tgctcgtctt cgacgcgtgg tggacgtgac ggggtgaatg
     2701 ggcgtgcggc gcgtactgca tgcggccaag ctgcagatcc cacgcgccgg acaagcccgt
     2761 ctccttgttg acccagaacg tccgttcgcg gaaccgcttc tcgtccacga gcgacctgag
     2821 tcgcgactcc tcgaagacgt agctggcggg cgttccctcg cccgtggatc gcccggtgtg
     2881 cttgggcacc gtgacgagca ggagagtcag agtcgtggcc gacgcggtca ccgcggcttc
     2941 atcgccgggc tccacataga cgcccgcgcg ccgtccggtg cggtgctcag tggtcccgcc
     3001 cgcgcttgac agcgtcaacg tcgcgtccgt gccctcgagg atgtaggcga cctgcgtctt
     3061 gtcgccacga gcgacgcgat acgtcttccc cggctcgacc tccctgacag caaccgcaac
     3121 cctcgtcccg tcgtcggccg ggttgagcac gtgtgtcacg cgcccttccg ggctcggagc
     3181 gttggccttg tccgggatgg cgatcacttt gatcattcga tgctcctacg ccatgacctt
     3241 ggggcgatac gccacccgcg cgacttccga gatcggcgcc ggctgcacgg tggcgcgaat
     3301 ctcgacctgg acgtggcgct ccacggtggg cttggacgat ccccggccct cctcacccca
     3361 gagcagcgtg acgtcggtgc cgggctcggc gtgctcgttg tcgaccacgg ccagcgagat
     3421 gatcgcgcgt tcgttgtacg tatagccggt gtaggtcgat acgcccaccg tcttgcccgc
     3481 cttcatcacc ttgtcgaacg gcagcgtcgc gtagttggcg agaggaagat cgatgtactt
     3541 cgcggcatcg cccgaatcgg acagcgacct gaagacccgt gcgacgtcat cgccgttcca
     3601 gacgagcgtg accttcttcc gcttctgcgc catccgttcg agcgcttctc gtccgatgaa
     3661 attgtggtcg aacttgacga acggcccgta gcccagatcg tacggcgtca ggtagtagtc
     3721 cctgatgtcg tcggagtaga agctcccgcc cagagacgcc atcgcttcgt agctcgtcgg
     3781 gcccagccac tgccggtacc ccttcatgcg ttcgccgacg tagactgccg gcacggggga
     3841 tggaatccat cccgattcga ggcaggtggt gggataggcc cgcgagccga cgcgccggat
     3901 cccgaactcc cggcctgctt cgaggatggc gtcgcggacg gcctcggcgt gctcccacgg
     3961 gccccacatc tcccaccccg gttgacccac catgccgtga cgcagcgcgc gcacgtcgtg
     4021 gcccgcgatt gcgaagacgc ccatgttgaa gaaccggatg tcgggcgtgg gcttcccggt
     4081 gaccttctcc atgacgcgga ccgcgtgcgg gccctgaacc tggtatctga aggatcgccg
     4141 cgtgcctcga ttcattgccg atctctcgtc gcgctcggcg gtcaccttgt agccgcccgt
     4201 ttcgacgttg aactgcaccc agttgagcgc cgagggacgc ccgacgaggc tcagccggtt
     4261 gtcgccgagg tagaacagga tcgcgtcgcc gatcacgtat ccctcgtgat tgcacgcgat
     4321 gaactgcttc gcctgatcga ccttgaacgt cgtgaagctg ttgacgccga ggtcggacag
     4381 cagcttgagc acgtccctgc cctcgacgta caggtccgtc atgtggtggg actggtcgaa
     4441 cagcacgcag ctctcgcgcc aggcccgctg ttcgtccctc cagttggtgt actcgggcgc
     4501 gacgacagga aacgcgtagg gtccgatctg tgaattgcgg agcatctcga ccgggcttcc
     4561 ggcagtccgc agcttgtctt ccaggctctg ggtcatctca actggttgct cctcgagagg
     4621 gacggaatta tatcgaactc ggcgcggctc acttcgtgtc caccctcagc atccgcatca
     4681 gctcgatggg aagcgggaag atgatcgtcg agttctgctc ggcggcgatc tccgtgagcg
     4741 tctgcagata gcgcagcgtc atggccgccg gctgccggtc gatgatggcc gccgcctgcg
     4801 ccagcttgtc cgaggcggtg agctcaccct cggcgtggat gatcttcgcc cgcttctcgc
     4861 gctcggcctc cgcctggcgc gcgatcgcgc gctgcatgtc ctgcggcagg tccacgtgct
     4921 tcacctcgac cgccgacacc ttgatgcccc acgggtcggt ctggttgtcg aggatgtgct
     4981 gcagctgctg gttcagccgc tcgcgttcgg ccagcagatc gtcgagctcc acctgcccga
     5041 gcacgctccg cagcgtcgtc tgcgagagct gcgacgtcgc gtacatgtag ttctcgactt
     5101 ccacgacggc cttgcttgga tcgatcacgc ggaagtagac gacggcgttc actttcagcg
     5161 acacgttgtc gcgcgtgatg atgtcctgag ggggcacgtc gagcacgacc gtgcgcaggc
     5221 tcattcggac gtaccggtcg atggggcgga agacgaggac gatgcccggc cctttcgggg
     5281 acggaagcag cttgccgagg cggaagacga cggcccgctc gtactcgttg aggatctgga
     5341 tcgagctgag cgcgtacagc acgagcagca ccaggaacac cagggctggg gaaatcggca
     5401 gaccctccat cagtcatctc cttttcgttg cgcggcttct gcgggctcga cggtgagtgt
     5461 cagtccgtcc acgcgggtca cgcggaccgg gtggccggcc ggcaccgcca cgagactgac
     5521 cgcgcgccag atctcgccgt gcacgtcgac cagtcccggc tctccgggcg ccagcatcgt
     5581 gcggacccgg gccgtctggc cgacgagggc cgatgcgccc gtcgccgcgg gctgacgctg
     5641 cgccttcaac gcaaggcggc cgaggaagag aaagagcgcg ctcacgctga gcgcggccgg
     5701 cacgatgatt ccgagcccga cccgtacgcc cgggacgacg tcggtcagca tcaccgagcc
     5761 gatcagcagg ctcacggtgc cgcccacgcc gagcgcgccg aagctcggca ccttcagctc
     5821 gagcacgagc agcgcgagcc cgaagatcac gagcagcacg cccgccgtgc ttacgggcag
     5881 gatctggaac gcgaagaagg ccagcaggag acatacaccg ccgatgaccc ccggcgcgat
     5941 gagtccgggg ttccagatct ccaccgtcag ccccagcagc ccgagcgtca tgagcatgta
     6001 cgcgatctgc ggatgggcga tggcactcag gaaccattgc cggcgggtca tctcgatccg
     6061 ccgtatctcc gcgcctttcg tgtgcacagt cgtggaccgg ccgtcgaagc gggtgatggc
     6121 gcggccatcg agctgcgcaa ggatctcgtc cacgtctttg gcgatgagac tgatgagagg
     6181 cggcttgacg tcgagcgcct cgcgatcggt gaacgcgcgg ctctcgagga cggcttccgc
     6241 cgccagcgcg acgttgcggc ctcgcgcctc cgccagcgac cgcgcgtacg ctgccgcatc
     6301 cgccgccgct ttcttcgacg tcgtctcgtc catcttctcg ttcgttcccg agacgggatg
     6361 cgcggcgccg gcgtgcgtgc cgggcgccat gaccgcgacg tcggcggcga gcagcaggat
     6421 gaaaccggcg gacgcggcgc gctcgcccga aggcccgacg aagacgacga cgggggcgcg
     6481 ggacgtgatc atgcgcgaca ccatcgtccg agtcgaatcc agcaggccgc cgggagttcg
     6541 caggaccagc accaccagct cggcgccgga ggtgtcggcc acgtcaacgg cgctcgtgaa
     6601 gtactcggcc gtgaccgggt ggatgatgcc gtcgatctcg gtgacgatga cctgccgcga
     6661 agccggtgcc ggttcctgcg cctggacccg gccggatgcg cagcacaaca ggaccgccgc
     6721 gacgagcagc cgcagtcgtg gcatggggct ccgattgtac gtcgtggccg cggcgacaca
     6781 aggggacatc agcctggggg tcggtgctac actccgccct ttgactgcct aactgtcaca
     6841 ggagcctgtc atgttgaagg aattcaggga cttcgtcgcc cgcgggaacg tcctggatct
     6901 ggccgtcggc gtgatcgtgg gcgcggcgtt cggcagggtc gtgtcctcgc tggtccaggg
     6961 cgtgctcatg ccgccgctcg gcctgctgct cggccggacc gacttttccg gcctgttcct
     7021 ggttctcgac cgttcgaagg acctgccggc aacgctgaag gaggcgaccg acaaaggcgt
     7081 tccggtgatc gcgtacgggc agttcatcaa cgagatcgtc agcttcctga tcgtggccct
     7141 ggcggtgttt ctgctggtgc gtcaggtcaa ccagctcaaa cgccggctcg agggaccgcc
     7201 gccgccggcg gcccctccgg ccacgaagga ctgcccgttc tgcgtgtcgg ccatcgccat
     7261 ccgcgcgact cgatgcccgc actgtacgtc gcagctctaa cttacatctc cacctgcacc
     7321 cccagctcca ccacgcgctc cgtgggcagc cggaagaacg ccgtggcgcg gacggcgttg
     7381 cgcgccatga cgacgaacag ccgctctcgc cacacggcca tgcccggccg ccgcgtgatg
     7441 atgatcgtct cgcgcccgag gaagtaggtg agatcgtcga ggtcgagctc gacgccctgt
     7501 tcgcgggcct tcagcagcgc gtccgggacg tccggatcct gcatgaagcc gtactgcgcg
     7561 cgcacgttgt agatcccgtg gcccagcacc tgtgcctgga cctg
//
LOCUS       DBNGSH010000053         2881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2041216, whole genome shotgun sequence.
ACCESSION   DBNGSH010000053 DBNGSH010000000
VERSION     DBNGSH010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2041216"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..640
                     /locus_tag="AC16CY_01110"
     CDS             56..640
                     /locus_tag="AC16CY_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3352401.1"
                     /translation="MNVRRWMWLTTIILLVVLTVTACAKKIPPPPPPPPEPPAAPAPP
                     PPPPPPPPPSVPKQPPAAPGPLSEEEVFSRKTLEELNAEMPLADVSFDYDQSAIRDDQ
                     RALLQKNADYLRRWTSVRVTVEGHADARGTNEYNLALGERRGNAVKDYLAGLGVTADR
                     MVVISKGEEAPLCKEETESCWLRNRRGHFIITAK"
     gene            complement(627..1355)
                     /gene="pyrF"
                     /locus_tag="AC16CY_01115"
     CDS             complement(627..1355)
                     /gene="pyrF"
                     /locus_tag="AC16CY_01115"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR3352402.1"
                     /translation="MGKLLVALDVESGARALELATALRGLADGVKVGSRLFTLEGPSL
                     VRRLVDAGAKVFLDLKFHDIPNTVAQAVEAATATGAWMVNVHASGGVAMMQAAAKAGA
                     EAATRAGRPRPLVIGVTVLTSMDQAALRENGVDRPLLEHVVALARMAQGAGLDGVVAS
                     PQETAAIRAACGPGFAIVTPGIRGAAAGPSKDDQARTMGPAEALRAGASYLVVGRPII
                     GAADPRDAAARIVEELAAAGATSP"
     gene            complement(1340..2278)
                     /locus_tag="AC16CY_01120"
     CDS             complement(1340..2278)
                     /locus_tag="AC16CY_01120"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR3352403.1"
                     /translation="MDLSVEIGSLRLSNPLIAASGCYGYGVEYADAVDLSSLGAVVSK
                     GLFLAPREGHPPPRIVETPSGMLNAIGLQGIGVHRYIAEKLPELRRLRATNIINICGS
                     TLDEYVELARILSDAEGVHALELNISCPNIKEGGITFGCSLHGTFDVVSAVKKVTRLP
                     VIPKLTPNVTDVASIAKAAEEAGADAVSLVNTFLAMAIDVETRRPKLSNVVGGLSGPA
                     IRPIAVRMVYECRRAVRIPVIGMGGIASAEDALEFIIAGATAVQVGTANFVDPFIWSK
                     LTAGIEEYMQRHSLARIRDLVGSIDTGTREKQWVSS"
     gene            complement(2354..>2881)
                     /locus_tag="AC16CY_01125"
     CDS             complement(2354..>2881)
                     /locus_tag="AC16CY_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HIR3352404.1"
                     /translation="CLGPLGRPFEPVDPPAEAWMVAGGVGLAPFATLAEALLARGTST
                     TLFYGARSGRELFYLDWFASRGVRVVLTTEDGSLGERGRITAPLDRELRRAAGDVMVY
                     ACGPERMLEAVGTVARSHHTPAQVSVERVMGCGMGGCYSCVIPVRERNGGQHYVRSCL
                     AGPVFSAADIVWDHE"
BASE COUNT          482 a         1020 c          929 g          450 t
ORIGIN      
        1 acgctaagct caccacgctc gcccaccttt tcacgatctc acacaggaga tatcgatgaa
       61 cgtgcgcaga tggatgtggt tgaccacgat catcctgctt gtcgttctca ctgtgacggc
      121 ctgcgcgaag aagataccgc cgccaccgcc gccgccgccg gaacccccgg ccgcgcccgc
      181 accaccgcct ccgccgccgc caccaccgcc gccgtccgtg ccgaaacagc cgccggcagc
      241 acctggcccg ctgagcgagg aagaagtgtt ctcgcgcaag acgctggagg agctcaacgc
      301 cgagatgccg ctggccgacg tgtcgttcga ttacgaccag tcggcgatcc gcgacgatca
      361 gcgcgcgctt ctacagaaaa acgccgacta cctgcgccgc tggacgtcgg ttcgcgtgac
      421 ggtcgaagga cacgccgatg cccgcggcac caacgagtac aacctggcgc tgggcgagcg
      481 gcgcggcaac gccgtcaagg actacctggc cggcctgggc gttacggccg atcgaatggt
      541 cgtgatcagc aaaggcgagg aagcgccctt gtgcaaggag gaaaccgaga gctgctggtt
      601 gcggaaccgc cgcggccact tcatcatcac ggcgaagtag cgcccgcggc ggcgagctct
      661 tcgacgattc tcgccgcggc gtcgcgtggg tcggcggccc cgataatggg ccgcccgacc
      721 acgaggtagc tggcgccggc ccgcagggcc tcggccggcc ccatcgttcg agcctggtcg
      781 tccttgctgg ggcccgcagc agcccctcgt attcctggcg tcacgatcgc gaaacccggg
      841 ccgcacgcgg ccctgatggc ggccgtttcc tgcggcgatg ccaccacgcc gtcgagcccg
      901 gccccctgcg ccattcgcgc cagcgccacc acgtgctcga gcagcggccg gtcaacgccg
      961 ttttcccgca gcgctgcctg atccatgctc gtcaggaccg tgacgccgat gacgagcggt
     1021 ctcggccggc ccgcgcgcgt cgccgcctcg gcccccgcct tcgcggccgc ctgcatcatg
     1081 gccactcccc ccgaggcgtg aacgttgacc atccacgcgc cggttgccgt cgcggcctcc
     1141 acggcctggg caacggtatt gggaatgtcg tgaaacttga ggtcgaggaa cacctttgca
     1201 ccggcgtcga cgagccggcg gaccagcgac gggccctcga gcgtgaagag ccggctgccg
     1261 accttgacgc cgtccgcgag cccgcgcagc gcggtcgcga gctcgagcgc gcgggcgccc
     1321 gattcgacgt cgagcgcgac taggagctta cccattgctt ttcgcgcgtg ccggtgtcga
     1381 tcgatccgac gaggtcacgg atgcgggcca gggagtgccg ctgcatgtac tcctcgatgc
     1441 ccgctgtcag cttcgaccag ataaaggggt ccacgaagtt cgcggtaccg acctgcacgg
     1501 ccgtcgcgcc ggcgatgatg aactcgaggg cgtcctccgc gctggcgatc cctcccatgc
     1561 cgatgacggg aatccgtacg gcacgtctgc actcgtacac catgcgcacg gcaatcggcc
     1621 tgatggccgg cccgctgagt ccgccgacga cattcgacag cttcggccgg cgtgtttcga
     1681 cgtcgatggc catcgccagg aacgtgttga cgagcgacac ggcgtcggct ccggcctctt
     1741 cggccgcttt cgcgatcgac gctacgtcgg tgacgttggg agtcagcttc gggatcaccg
     1801 gcagacgggt cactttcttc accgcgctca cgacgtcgaa cgttccgtgc aggctgcagc
     1861 cgaaggtgat ccccccctcc ttgatgttgg ggcacgagat gttcagctcg agcgcgtgca
     1921 cgccctccgc gtccgagagg atgcgagcca gctccacgta ctcgtcgagc gtgctgccgc
     1981 agatgttgat gatgttcgtc gcccgcaggc gacgcagctc ggggagcttc tcggcgatgt
     2041 agcgatggac gccgatgccc tgtagcccga ttgcattcag cattccagac ggggtctcga
     2101 cgatgcgcgg cggcgggtgc ccttcgcgag gggcaaggaa caggcccttg gagacaacgg
     2161 cgccgagcga agagaggtcc acggcatccg cgtattcgac gccgtatccg tagcacccgc
     2221 tcgcggcgat caacgggttg gagagccgga gcgatccaat ctctacagac agatccacgt
     2281 gaactcccaa ctcccaaagc cgaactccca atcgacccga ttcccttggg tggtgggagt
     2341 tggaggttgg gagttactca tgatcccaga cgatgtcggc ggcgctgaac accggtcccg
     2401 ccaggcacga gcgcacgtag tgctgtccac cgttgcgttc gcgcaccggg atgacgcagc
     2461 tgtagcaccc gcccatgccg catcccatca cgcgttcgac cgacacctgt gccggtgtgt
     2521 gatgggagcg cgccactgta ccgacggctt cgagcatccg ctccggtccg cacgcgtaga
     2581 ccatgacgtc tccagcggcc ctccgcagct cgcgatcgag tggagcggtg atccggccgc
     2641 gctccccgag gcttccgtct tccgtcgtga gaaccacgcg gacgccgcgc gatgcaaacc
     2701 agtcgagata gaagagctcg cgtcccgagc gagcgccgta gaagagcgtc gttgacgtgc
     2761 cgcgcgccag aagcgcttcg gccagcgtcg caaacggcgc gagccccacg cccccggcga
     2821 ccatccacgc ctccgcgggc gggtctaccg gctcgaaagg acggcccaga ggccccaggc
     2881 a
//
LOCUS       DBNGSH010000054         3459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3569531, whole genome shotgun sequence.
ACCESSION   DBNGSH010000054 DBNGSH010000000
VERSION     DBNGSH010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3569531"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /gene="panB"
                     /locus_tag="AC16CY_01130"
     CDS             complement(<1..803)
                     /gene="panB"
                     /locus_tag="AC16CY_01130"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3352405.1"
                     /translation="MSTRRIRVPEFREKKARGEKIAMLTAYDATMARLLDRAGVDALL
                     VGDSLGTVVLGYDTTLPVTLDDMIRHTRAVSHGAERALVVADMPFLTYQVSVSEAVRN
                     AGRLLQEGGAAAVKLEGGRPVLDVVRRLVDVGIPVMGHLGLQPQSVHQAGGYVKRATR
                     PQDADALVADAIALQDAGAFAVVLESIPDEVAKAATSSVSIPTIGIGAGPDCDGQVLV
                     SYDMLGLFDRFVPSFVKQYAKLSDTIGAATKSYVDDVRAGRYPQTLTKVS"
     gene            complement(813..2504)
                     /locus_tag="AC16CY_01135"
     CDS             complement(813..2504)
                     /locus_tag="AC16CY_01135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3352406.1"
                     /translation="MVLRFSRFVQSACAALLLLPGIAGAQQGTAARPSTGYNVFVRGT
                     LLGREVVTISRDQEGTTITTQGRLSPPLNVTLRNAEFKYRPDWTPVSFVLDATTSSGN
                     MSLRSTFANTAASTQGTLGTQPVSMTHTIVPRTIVHANGVFASYVALARRLAENSEEG
                     AEFRVYVVTQMEIGILVKAIHRERMQIGTSMLDVRRFELVYRNPAGDVLTNLTVTDDG
                     TLVSVNIPSESVDLVRDDLASSTSRTQVYSNPGDEAVIIPATGFNLGATLTKPKGTNA
                     TAAGKGARLPAVVLLAGAGIGDRDGFIQGVPILTQTAGAIADAGMLAVRFDKRGNGQS
                     GGRSESATLGDFAEDARVIVRWLERRPDVDPKRIAVVGHSEGAWVAMLAASREKKIAA
                     LVSIAGASGTGAELVLEQQRHTLDQLNLPPEEREKRIALQKQIQSAVITGQGWDEVPA
                     DVRKGADTPWFQSLLAFDPAKVFEDVRQPLLFVHGELDREIPVAHTERLSALAQKESD
                     SKSIEAVIVRGVNHLLVPAVTGEKSEYGTLPDRHVSKDVTMAVTNWLTRTFAAIR"
     gene            2592..>3459
                     /gene="ygfZ"
                     /locus_tag="AC16CY_01140"
     CDS             2592..>3459
                     /gene="ygfZ"
                     /locus_tag="AC16CY_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR3352407.1"
                     /translation="MDFQDGYRAIKLRAAIGAVSPRLQIAVSGADRAAYLQGLLTNDI
                     QSLVPGTGCYSAWLSPQGRMLTDMHVLESGGMMLLDVPAEVLQPTVDRLEQFIFTEDV
                     HVGSMAASLAGVWLHGPEAPAVLERALEGARGLADWQEYRHAQFERGDQPVSVARISQ
                     LGVPGYCVYVERSLQTTLVPALEAAGAVLAPPDAIEAARIEAGYPVFGVDMTDDTIPL
                     EAGIEQRAISFSKGCYVGQEVIIRVLHRGHGRVVRKLMALHIDGPPPARGAKLYAGDR
                     EIGIVTSAASSPL"
BASE COUNT          535 a         1163 c         1179 g          582 t
ORIGIN      
        1 gagaccttcg tcaacgtctg cgggtatcgt ccggcgcgta cgtcgtccac gtacgacttc
       61 gtcgcggcgc cgatggtgtc tgacagcttt gcgtactgct tcacgaacga cggaacgaag
      121 cggtcgaaca gccccagcat gtcgtagctc acgagaacct ggccgtcgca atcgggcccc
      181 gcgccgatgc cgatcgtcgg gatgctcacg ctggaggtgg ccgctttcgc gacctcgtcg
      241 ggaatcgact cgagcaccac cgcgaaggct cccgcatcct gcagcgcgat cgcgtcggcg
      301 acgagcgcat cggcgtcttg cggccgggtg gcgcgcttga cgtatccgcc ggcctggtga
      361 accgactgcg gctgcaagcc gagatgtccc atcaccggaa tccccacgtc cacgaggcgc
      421 cggacgacgt cgagcaccgg acggccccct tcgagcttga cggccgcggc gcctccttcc
      481 tgcagcagcc gcccggcatt gcgaacggcc tccgacacgc tgacctggta ggtgagaaac
      541 ggcatgtcgg ccacgacgag cgcgcgctcc gctccgtgcg agacggcgcg cgtatggcga
      601 atcatgtcgt cgagcgtcac ggggagcgtg gtgtcgtatc cgagcacgac cgtgccgagc
      661 gagtcgccga cgagcagcgc atcgacgccg gcccggtcca gcagacgggc catcgtggcg
      721 tcgtatgcag tgagcatcgc gatcttctcg ccgcgtgcct tcttttccct gaattccggg
      781 acgcgaatcc tgcgcgtgct catgaggggt tgtcagcgga tcgcggcaaa cgtcctggtc
      841 agccagttgg tgacagccat cgtgacgtcc ttgctgacgt ggcggtcggg gagcgtgccg
      901 tactcgctct tctcgcccgt cacggcgggg acgagcaggt gattcacgcc gcggacgatg
      961 accgcctcga tcgacttcga atcggactcc ttctgcgcca gagcggagag ccgctcggtg
     1021 tgcgccaccg ggatctcgcg atcgagctcc ccatgcacga acagcagcgg ctggcgcacg
     1081 tcctcgaaca ccttggccgg atcgaaggcc agcaggctct ggaaccaggg cgtgtcggcc
     1141 cccttcctca cgtcggccgg cacctcgtcc catccctgtc ctgtgatcac ggctgattga
     1201 atctgcttct ggagcgcgat ccgcttctcg cgctcctccg gcggcaggtt caactggtcg
     1261 agcgtatggc gctgctgctc gagcacgagc tcggcgccgg tgccggaggc gccggcgatc
     1321 gacaccaggg ccgcaatctt cttctcgcgg gatgcggcaa gcatcgccac ccaggcgccc
     1381 tcgctgtgac ccaccaccgc aatccgcttg ggatcgacat ccggcctgcg ctcgagccac
     1441 cggacaatca cccgcgcgtc ttcggcgaag tcgccgagcg tggccgattc cgaccgaccg
     1501 ccgctttgcc cgttgccgcg cttgtcgaac cggacggcca gcatccccgc gtcggcgatc
     1561 gcgccggcgg tctgcgtgag aatcggtact ccctggatga agccgtcccg atccccgatc
     1621 ccggcgccgg cgagcagaac gacggccggc agccgagcgc ctttgccggc agctgtggcg
     1681 ttggtcccct tgggcttcgt cagcgtggcg ccaaggttga atccggtcgc cgggatgatg
     1741 accgcttcgt ccccggggtt ggaatacacc tgcgtgcgcg aggtggacga ggccagatcg
     1801 tcgcgcacga gatccaccga ttccgacggg atgttgacgc tgaccagcgt cccgtcatcg
     1861 gtgaccgtga ggttcgtcag cacatcgccc gccgggttcc ggtagaccag ctcgaaccgc
     1921 cggacgtcga gcatcgaggt cccgatctgc atccgctcgc gatggatcgc cttcaccaga
     1981 attccaatct ccatctgcgt cacgacgtag acccggaact ccgccccctc ttccgagttc
     2041 tcggcgagcc gccgcgcgag cgcgacgtag gacgcgaaga cgccgttggc gtggacgatc
     2101 gtcctgggca cgatcgtgtg ggtcatcgag accggctgcg tgccgagcgt tccttgcgtc
     2161 gaggctgcgg tattggcgaa ggtgctgcgc agcgacatgt tgcccgagct ggtcgtggcg
     2221 tccaggacga acgagaccgg cgtccagtcc ggacggtact tgaactcggc gttgcgaagc
     2281 gtgacgttga ggggaggcga cagccggccc tgcgtcgtga tggtggtgcc ctcctgatcg
     2341 cggctgatgg tcacgacctc gcgcccgagg agcgtgcccc tgacgaagac gttatagccg
     2401 gtggacggcc gtgctgccgt cccctgctga gccccggcga tcccgggcag aagcagcagg
     2461 gccgcacacg cggactggac aaaccggctg aagcgaagca ccatcgactc gatcgtatca
     2521 ggtctggagt agtgtccggc ttcagccgga cgggtacgta gagtccggct tcagccggac
     2581 cgcaggggac gatggatttc caggacggct acagggcgat caaactgcgg gcggcgattg
     2641 gcgccgtgtc gccgaggctg cagatcgcgg tttcgggcgc cgatcgggcc gcgtacctgc
     2701 agggcctgct caccaacgac atccagtcgc tcgtgccagg cacgggctgc tactcggcct
     2761 ggctgtcgcc ccaggggcgg atgctcaccg acatgcacgt gctcgagtcg ggggggatga
     2821 tgctgctcga cgtcccggct gaagtgctgc agccgacggt cgatcggctc gagcagttca
     2881 tcttcaccga ggacgtccat gtgggctcga tggctgcgag cctggccggc gtctggctgc
     2941 acgggcccga agcgccagct gtgctcgaac gcgcgctcga gggcgcgcgc gggctcgccg
     3001 actggcagga gtaccggcac gcgcagttcg agcgtggcga tcaaccggta tctgtcgcgc
     3061 gtatctctca actcggcgtg cccgggtact gcgtgtacgt ggagcgatcg ctccaaacga
     3121 cgctcgtgcc ggcgctggaa gccgcgggcg cggtgctggc gccgcccgac gcgatcgagg
     3181 ccgcgcggat cgaagccggc tacccggtct tcggcgtgga catgaccgac gacacgattc
     3241 ccctcgaggc gggcatcgag cagcgcgcga tctcgttctc gaaggggtgc tacgtggggc
     3301 aggaggtcat cattcgcgtg ctgcaccggg ggcacggccg cgtggtcagg aagctcatgg
     3361 cgctccacat cgacggtccg cctcccgcgc ggggcgcgaa gctgtatgcg ggcgatcgcg
     3421 agatcgggat cgtgacgagc gctgccagct ctccactcg
//
LOCUS       DBNGSH010000055        18792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3824, whole genome shotgun sequence.
ACCESSION   DBNGSH010000055 DBNGSH010000000
VERSION     DBNGSH010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 18792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18792
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3824"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="AC16CY_01145"
     CDS             <1..386
                     /locus_tag="AC16CY_01145"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3352408.1"
                     /translation="ALESVGLGDRMHHHPNQMSGGQQQRVAIARALVTNPLLLLADEP
                     TGNLDSRTSVEVMDIFQRLNVEKGLTIVLVTHEHDIAEYGTRIVAFRDGRVRTDEPVK
                     TRRTAAVELEHLAAQADEADQTLTA"
     gene            394..1614
                     /locus_tag="AC16CY_01150"
     CDS             394..1614
                     /locus_tag="AC16CY_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352409.1"
                     /translation="MALLMTFVIAVRALRRNAMRTALTALGMIIGVAAVIVMMALGSG
                     ARNAIEQQIRNVGSNLVTVNAGSNRNWNVRGGQGGSSTLTVGDAEAIRESIPGVRYLT
                     PGLNIRVQAISSLGNWQTQAQGSNQELPAIRSWPTEMGSFFTEDDVKRASKVVVLGSV
                     ARDQLFGAGVDPVGETLRVRNQPFRVIGVLSKKGQASFSGDQDDTMIVPFTTVQKKLT
                     GQDYLREITVSAEPGVDLNKMVAQIQALLRERHAIPPGGDDDFLVRTLDEMAGVLTET
                     TNTMTYLLAGIAAVSLLVGGIGIMNIMLVSVTERTREIGLRLSVGARDLDVLLQFLTE
                     AMVLSLAGGAIGVALGFGASRVLTQVMQWSTLVTVDAVLLSFGCAAAIGIFFGFYPAR
                     KAAGLDPIDALRYE"
     gene            complement(1636..3456)
                     /locus_tag="AC16CY_01155"
     CDS             complement(1636..3456)
                     /locus_tag="AC16CY_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3352410.1"
                     /translation="MNVRRALFAATLVAATLAPSAASAQKDLFVDALIAFRTALGGTY
                     GDEGLQLEAALQRMASSLAAWDREAAAAEAELRSGLPGAPSEDRLRRRIALASLMLDR
                     DRWAEALENLDAAVAENRNHGSVYLARGLVRDRAGDHVGAVRDFERAWELDRSDPVKA
                     YLHVTHGLAAGALDDPNPPLDALLAAHRAAAASAPRGFMEIGLLRDRAEWPVLAPAAY
                     AEGFAFVAGGRYGEALASFRAAVARDPLRTDPAAKTGQLARGSASLRDGQYTAAITHL
                     ETVVTATPASAEAHRLLGTAYHLAGRPAESLERLMTAIRLAPSDERARIALARELLAL
                     EREEAEGALREVIATLPASAESRWTLAQQYVKSGRDAEAIAEIEALLTFPLLAGRGQL
                     DWLLAGLYSRHLDFETSIRVLSERVRLDLNNPVVHKELGLAYLRVGSRPQALAELLMT
                     ALFAPDDLETLERIGQIHLDDERYADAEAVLRRVVTRAPDRARPRYALGTTLLRLGRT
                     EEGQAELAAFERLIVAKREAEVQKIEFERLLWNAERSAGDGRFDEAVASLQQAAAIVD
                     DDPRVYQLLASAYGRLGRTDDRARALAAYERLAGKRAIEP"
     gene            3558..5252
                     /locus_tag="AC16CY_01160"
     CDS             3558..5252
                     /locus_tag="AC16CY_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3352411.1"
                     /translation="MGWVTCRLPFVFAAVGPALLVYSTIPLAQERPLRIIDVASQSGI
                     DLLNVSGSPAKDYIVEANGNGVAFFDYDNDGDMDALLVNGSTIDRVARGGDPMVALYE
                     NDGRGRFRDVTSNTGLNRRGWGTGVCIADVDNDGFQDLYVTALGPNVLWRNTGERRFL
                     ATDQASDPRWSTGCAFGDYDRDGDVDLYVANYLAFDVAKVPRKGETSCGRFMNIVALC
                     GPRGLTPQPDALYRNTGDGHFVDVTQAAGVAQPGRFGFGVVFSDLDDDGWPDIYVAND
                     SMPNFLFRNRRDGTFVEEGLLRGVAVSRDGREQASMGVDAADYDGDGDFDLVVTNFAQ
                     DYTTVYHNLGGGTFADRSFESGVGAYGPSLGWGVGFVDIDNDGLLDLFVSNGHVYPDV
                     EQTGTSTYRQRSLLFRNQGRGYLRKVTTSEGGLALARSSRGAAFGDYDNDGDIDVLIS
                     NIDDRPTLLRNDTSGGSWITMRLVGVKSNRDGIGAKVAVVAGGRRQTAEVRAGGSYVS
                     HNDMRLHFGLGNQDRVDVVEIRWPSGLVERVDGLRANRFYQAREGSGIQQDPLLTR"
     gene            5273..6163
                     /locus_tag="AC16CY_01165"
     CDS             5273..6163
                     /locus_tag="AC16CY_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352412.1"
                     /translation="MALRLLLLVCLPALLATDAEAQAQLQFFATIVDSSGAPGTAVQP
                     GNIQVTENGVEARLVKIEQVNWPLKVQLLVDNGVGLGRENLVSLRAGVRGFLEALAQG
                     TEVTFVTTSPQPRFLVRATTDREALLQGLGQLTPDSGSGRFVESLSEATQRIERDKTD
                     FFPVIVSVATTSSDANARESMIQQLMGRLQKRPTTVHVVLLTRAAGRTASDGYIQHEL
                     GLNVTKFTGGRFENIGAGAQLETLLAEIGALVAKSNPGGASKFRITVERPGGASGDLG
                     KISVILSGGFVVTDLSLTSR"
     gene            complement(6178..7890)
                     /locus_tag="AC16CY_01170"
     CDS             complement(6178..7890)
                     /locus_tag="AC16CY_01170"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3352413.1"
                     /translation="MSRVCALVFLLLLPAPAAAQKGAFIEALLEFHSALSGTYGDEGP
                     RVEAALDRMDVALEAWNAESRLAEASLRAASDSSALARFFLNEGRPADALEAVEEAIR
                     LKPERAGLHMLRGVLLGADRRDADALASFQRAWALDPDDVVNAYLLADALSTRPSEPP
                     ERQLASLLAAIGSEPPSPMSGRDATRPDSTGQAVRPPAGPPQRPSVGTSTRRASFIQV
                     GLIDDSASEAPQFSRAAYADGVAALANGRYEDAIGLFRAAMVRDPLVVDHVARSGPAA
                     RGVAALRQGQTTDAVADLEAAVAAAPSSSEARRLLGVAHATAGNVDASLKWLAEAIRV
                     APADERAHIAMGRVLMEAGRIEEAERVLLDTLRLLPASGRTRWALADLYELTGRDADA
                     IDQLEQAATLTMIAGKTALLLRLASFAHELLDEERVISAFTRQTRLMRNNPLAHRSLG
                     LAYHRAGRDVEALVELLVSSHLGQEDAETLAVMGQIHLNAGRLTMAETMLRRAVAIAP
                     DSAPARYALSRTLLGLDKEEEGRQQLAEFERLQERLLDERRQKYENDLRERNERLRGE
                     TTGK"
     gene            complement(7900..9639)
                     /locus_tag="AC16CY_01175"
     CDS             complement(7900..9639)
                     /locus_tag="AC16CY_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3352414.1"
                     /translation="MVLVMPRWVAVLPGLAFTTLAISGTAGQWPDASKRVAFDDVSKR
                     SGLVFTHVNGASPDKHLAEIMGSGGLFFDFDNDGWIDVFLVDGGSKADPRVSGTARHR
                     LFHNRGNGSFEDVTANSGIRHLAYGMGACAGDYDNDGLTDLYVTTNDGPNVLYRNRGN
                     GAFTDITTTARVGSPLWSTSCAFADVDRDGRLDLFVTNYVQGAEENPFCGNTHPEKLR
                     MYCHPLNFRPSPSVLYHNAGDGAFTDVSAGAGIASFTGNGLGVAVADYDDDLWPDVFV
                     ANDGVPNFLFHNKGDGRFTELALVAGVAVASDGKARAGMGTAFGDVDGDGRLDLVVTN
                     HETEMHSLFRNAGNGAFTDATASSGIGPATLQHVGFGVAFADYDNDGDLDLAIVNGHV
                     IDNIAMRRSGAKHAQKRLLMQNTGAGRFRNVSPLSGPPFAVEKVGRTVVWGDVDNDGD
                     LDLLVTNNGQQAELLLNDGGNGGNALLLRLIGGSTSLTTGTMSNRDAIGARVRLTSGG
                     RTQVREVTSGSSYLGQNDPRLHVGLGAGTIVDRVEIRWPSGQTEVVQNVAANQVIVIR
                     EGAGIVASTRFQR"
     gene            9813..13370
                     /locus_tag="AC16CY_01180"
     CDS             9813..13370
                     /locus_tag="AC16CY_01180"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3352415.1"
                     /translation="MTLVLAVFVMLLPASALAQQGNIAGTVRDSSGAVLPGVVVEATS
                     PALIEKVRSGVTDETGQYRITNLPVGTYSVAFRLEGFSTVERANVVLSTGFTAPVNVT
                     MAVGQVAETVKVVAETPTVDVQSARQVASFTGADIRDLPTTRNIRSILTMTPGLTPSG
                     LGADCVGNVGVWCNNNIYNLGAHTDVNTAGAFDPPDQIAQDGLNQGRIMVDGTIINTG
                     GGAGIMGMTGGYVADVANSQEVNVQISGALGESETGGATINIVPRTGGNNFAGNYFVT
                     YTRGPLDSAGNGRTGWFSQNNGNYPEINNNYPLVSDVDTSAAFGGPIVRDHLWFFSAG
                     RMWQKNAFSSQVDRIWDNANAGIYGENYEADYSTDPLQLVNWTRNANVRVTWQATRKD
                     KVNFFWDEGFTCQDPCDGSVAAWTSRDGWWSGQVHPARLIQASWTNPLTNTILAEAGL
                     SVNRQLYDFSQHRYWTPNPDIPRVIELGSTVGYNYAQNAPLNTVNFNLFGVPSGPWSD
                     GIGGLAEIRNLNDLRPRASVSYVTGGHNMKIGYDGGYFSQTRQNRTNNPRLEYRYFTP
                     AATCFDAANPAASTCGNTSRYHPEDPYNSLRRPVPDQVKISTGPSAIENRVGYSGFYA
                     QDQWTLKRFTLSGAIRYDHSFSRYPGTCIGAAAGNDLVAGASPEPYVPVQVGGQYGGQ
                     REYCTPDNDGVSYHDVTPRWGVTWDLFGNGKTSIKYNQGQYLSGAGISGIYSSANPVE
                     RGKNTYTRTWSDLDGDRFPDCDLLNYSAQSPATGAVDACGGPNSVFIFPAGFEDPTRY
                     GRDPYSLDASGQLLGLQTTYCGSTELAIPAAVQSYCNAYGDTLIEGWGRRRSEWQFGL
                     GIQHEILPRLSAEVTYNRRSFANQTVNDALGLGCDRFNSTYTLEECNDLYLNHTHPDY
                     GFYTVTAPSNPDLPSGGGYVVRGLADPNALRFPGPTAVTILQTLEYSWNGVDTNFVWR
                     APGGLRVNGGTSTGRSVRDLCHAATDAPNVKVYDGAAVACNPHRRWDTNVRGTAAYTI
                     PKIDMLVSSVFQWRPGVERAANYTFNKNQLTWDASSAFRATQPCANPANGTGCLTSGN
                     AGSGGTTTTVNLLNTGEVYGEPYSIVDLKIAKNIKFANKRLNVGVDIYNLFNDDAIRS
                     YQDTLDTVDNPNTPVVEKYGQATSLLSPRFVRLSVQFDF"
     gene            13540..15210
                     /locus_tag="AC16CY_01185"
     CDS             13540..15210
                     /locus_tag="AC16CY_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3352416.1"
                     /translation="MTFALRTLTVCVLCLLGAAFPGAADYAIRLVDVPGGGGINLLNV
                     SGSPTKDYIVEGNGNGAGFFDYDRDGDLDAIVINGSTFDRLKSGGDPLTALYRNDGGG
                     RFSDVTASSGMNRRGWGSGVCVADYDNDGFEDVYITAFGPDVLYRNTGRGAFTATNQA
                     SDELWSTGCAFGDYDRDGHLDLYVANYVAFDRAKVPRRGAGGCKFMNIDAFCGPRPLP
                     GARDVLYRNQGDGTFVDATAKARTIEPGHYGFGVLFSDLNDDGWPDIYVANDSLPNLL
                     FRNRGDGTFSEEALLAGVAVSGDGREQAGMGVDAGDYDADGRLDLIVTHFAQDYTTLY
                     RNEGDGLFRDVSFESGMAATLGLPLGWGVGLLDLDNNGWLDVFQSNGHIHPEIAKTGT
                     STYRQRNQVFRNIGGKRFQHVTDDAGNALRVEKSYRGAAFGDYDNDGDLDVLVIPIDD
                     RPTLLRNDTAGGHWITLRLVGSKSNRDGIGARVTIEAGGRRQIAEVRSGGSYVSHNDM
                     RVHFGLGEARRVDRLAIRWPSGLVETATGLDGDRFYVAREGSAVRAGL"
     gene            15408..>18792
                     /locus_tag="AC16CY_01190"
     CDS             15408..>18792
                     /locus_tag="AC16CY_01190"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3352417.1"
                     /translation="MQRAVKVILALAAFVVLLPGASFAQEGQIAGTVRDSSGAVMPGV
                     LVEATSPALIEKVRATTSDASGQYRLTNLPVGTYSITFTLEGFNKWQREMIVLTSGFT
                     ASINATMAVGQLAETVTVLSETPTVDVQSARQALTLAGTDLAELPTNRNVNSLLALTP
                     GLTSGYAPGTLSGICSGGVGVFCNPGVTGFNVGDNDPTNMAQGRVLVDGQVINSGTAV
                     PIVGQTGGYTADIANAQEVAIQISGAMGESETGGASINIVPRTGGNRFAGNFFTSFTD
                     DSFFSQNNGAYPGIPAVFQPVISDHDIIGSFGGPIKRDTLWFYSVARKQGIHKLPVGV
                     DFWPNLWEGQWGYNYQPDRAEPRVEYKNIWRNVNARVTYQATQKNKFNFFWDEQDFCQ
                     DPCLGVVSVFTSPESWWSVQSYPNRLQQASWTNPLTNKILLEAGINITSQQYNTTEHR
                     EYTNPRAIPRVIETGETAGGDATAPRVNQFAGAGFFALTSGSLNSQINGGGAERRDLD
                     NYRMRASASYVTGTHNAKFGYDGGFYSQIQTNEANDVRMTYNYVWPAADCANTNSCGN
                     TSLQFPNNPTNTPLGPDGVAGTADDGRRPVPSNIVFNTGVGSLNDKVNYTALYAQDQW
                     TLKRLTLNGAVRFDHAASSYGETCVGPDRYVPIQTNGANFYCTPPSDGVSFNDVTPRM
                     GVVWDVFGTGRTSVKWNMGKYLTAAGISGIYSNANPARRTVNTLQRSWNDIDGDRLVD
                     CDVLNPAITPECGAFLFPNNDLTRFGRDPLGLDAAGTPIGLATTQCGRQEAGIPAAVQ
                     AYCAAYGESMIEGWGRRQSEWQFGIGVQHELLPRLSAEVVYNRRSYFNLTTSDQLGVG
                     CDRFNGAQDVRTCQQGNLDYTNPSYDFYMVNVPSDSRLPDGGGYQVIGLNTPKTSVPF
                     GAPTAQTLNPDLDYVWNGVDVNFVWRGPQGLRVNGGTQTGRSNRDTCFANLDAPAVSG
                     RPGAEYKGGETPAGPFNVPPAQPNGCQSVTPWLTTVKGSAAYVVPWVDVLVSTIFQSF
                     PGVERNAVMTFGKNDVIWMPGSESRATRPCANPLDGVGCLGPTGTQTTTFVNLLNNNE
                     MYGERVTSFDFKASKIIRFGNRRVTIGGDLYNVFNSDAVQ"
BASE COUNT         3275 a         6286 c         6187 g         3044 t
ORIGIN      
        1 ccgcgctcga atcggtcggg ctgggcgacc gcatgcatca tcatcccaat cagatgtccg
       61 gcggccagca gcaacgcgtg gcgatcgcgc gcgcgctcgt cacgaatccg ctcctgctgc
      121 tcgccgacga gccgacgggc aatctcgact cgcgcacgag cgtcgaagtg atggatatct
      181 tccagcggct gaacgtcgag aaggggctga cgatcgtgct cgtgacccac gagcacgaca
      241 tcgcggaata cgggacgcgg atcgtggcgt ttcgcgacgg gcgcgttcgc acggacgagc
      301 cggtcaagac ccgacgcacg gccgctgtcg aactcgaaca cctcgcggct caggccgacg
      361 aggccgacca aaccctaact gcttgaggac gccatggctc tgctcatgac attcgtgatc
      421 gccgtccggg cccttcgacg caacgccatg cggacggccc tgaccgcgct ggggatgatc
      481 atcggcgtgg ccgccgtcat cgtgatgatg gcgctcggca gcggggcgcg gaatgcgatc
      541 gagcagcaaa tccgcaacgt cggatccaat ctcgtgaccg tgaacgccgg ctcgaaccgg
      601 aactggaacg tgcggggcgg gcagggcggg tcgagcacgc tgacggtggg cgatgccgag
      661 gcaatccgcg agagcatccc gggcgttcgc tacctcacgc cgggcctgaa cattcgcgtg
      721 caggcgatct cgtcgctcgg aaactggcaa acgcaggcgc aggggtccaa ccaggaactg
      781 ccggcgatcc gctcgtggcc gaccgagatg ggctcgttct tcacagagga cgacgtgaaa
      841 cgggccagca aggtggtcgt gctgggttcg gtcgcgcgcg atcagctttt tggggccgga
      901 gtcgatccgg tgggcgagac gctccgggtg cgcaatcagc cgtttcgcgt gatcggcgtc
      961 ctgtcgaaga aggggcaggc ctccttcagc ggcgaccagg acgacaccat gatcgtgccg
     1021 ttcacgaccg tgcagaagaa gctgacgggc caggactacc tgcgggagat cacggtctcg
     1081 gcggagccgg gcgtcgatct gaacaaaatg gtcgcccaga tccaggccct gctgcgcgag
     1141 cgccacgcca tcccgccggg cggcgacgac gacttcctgg tgcggacgct cgacgagatg
     1201 gcgggtgtgc tgaccgagac gaccaacacg atgacgtatc tgctggccgg catcgccgcc
     1261 gtgtcgctgc tcgtcggtgg catcggcatc atgaacatca tgctcgtgtc ggtgaccgag
     1321 cggacgaggg agatcggact gcgcctgtcg gtcggggcgc gcgacctcga cgtgctgctc
     1381 cagttcctca ccgaggcgat ggtgctcagc ctggcgggcg gcgccatcgg ggtcgcgctc
     1441 ggcttcgggg cctcgcgcgt gctgacccag gtgatgcagt ggtccacgct cgtcaccgtg
     1501 gacgcggttc tgctgtcgtt cggctgtgcc gcggcgatcg ggatcttctt cgggttctac
     1561 cccgcccgaa aggcagcggg gctcgatccg atcgacgcac tccgatatga ataggctggc
     1621 gcgcctggcc cggcgtcagg gctcgatcgc gcgcttccct gcgagccgct cgtaggcggc
     1681 cagcgcgcgc gcacggtcgt cggtgcgccc cagtctcccg tacgccgacg ccagcaactg
     1741 gtagacgcgg gggtcgtcgt cgacgatcgc ggcggcctgc tggagcgatg cgaccgcctc
     1801 gtcgaagcgt ccgtcgccgg cggaccgctc cgcgttccat agcagcctct cgaactcgat
     1861 cttctggacc tccgcttctc gcttcgcgac gatcagccgc tcgaacgcgg cgagctccgc
     1921 ctggccctcc tcggtgcgac caagccggag cagcgtcgtt cccagggcgt acctgggccg
     1981 ggccctgtcg ggcgctcgag tgacgacgcg tcgcagtacg gcctctgcgt ctgcgtatcg
     2041 ctcgtcgtcg aggtgaatct gaccgatcct ttccagcgtc tccaggtcgt ccggagcgaa
     2101 gagggcggtc atgagcaact cggcgagcgc ctgcggacgg cttccgaccc tcaggtacgc
     2161 cagcccgagt tccttgtgca cgaccggatt gttcaggtcg agccgcacgc gctcggagag
     2221 cacccgaata gaagtctcga agtccaggtg gcggctgtag agtccggcga gcagccaatc
     2281 gagctggccc cggcctgcga gcaacgggaa ggtcaagagc gcctcgatct ccgcgatggc
     2341 ttccgcgtcc cggccgctct ttacgtactg ttgtgcgagt gtccaacgcg actcggccga
     2401 cgccggcaga gtggcgatca cctcgcgcag cgctccttca gcctcctccc gttccagggc
     2461 gagcagttcc cgcgccaggg cgatgcgggc ccgttcgtcc gaaggagcga gacgaatcgc
     2521 cgtcatcagt cgctcgaggc tctcggcggg tcgtccggcg aggtggtagg cggtcccgag
     2581 gaggcggtgg gcttccgcag aggcaggcgt ggcggttacg accgtctcga gatgggtgat
     2641 ggctgcggtg tactggccgt cccgaaggct cgcgctccct cgagccagct gtcccgtctt
     2701 cgccgcaggg tccgtccgca acgggtcccg ggcgacggca gcacggaagc tcgccagcgc
     2761 ctcgccgtag cgcccccctg cgacgaacgc gaagccctcg gcatatgccg ccggagcgag
     2821 cacgggccac tccgcgcggt cgcggaggag accgatctcc atgaagccgc gcggagcgct
     2881 cgccgccgct gcccgatgtg cggccagcag cgcgtccagg ggcgggttgg gatcgtcgag
     2941 tgcgccggct gcgagcccat gtgtgacgtg gagatacgcc ttgaccgggt cgctgcgatc
     3001 gagctcccag gcgcgctcga agtcgcgcac ggcccccacg tggtcgccgg ccctgtcgcg
     3061 caccagccct cgcgccaggt acacggaccc gtggttgcgg ttctcggcga ccgccgcgtc
     3121 gaggttctcg agcgcttctg cccaccggtc gcggtcgagc atcagcgacg ccagcgcgat
     3181 ccgacggcgc agccgatcct cggacggcgc accgggcaac ccgctccgca gctccgcttc
     3241 ggcggctgcg gcctcccgat cccacgccgc caggctggag gccatgcgct gcagcgcagc
     3301 ctcgagttgc agcccctcgt cgccgtaggt gcctcccagc gccgtgcgaa acgcgatcag
     3361 tgcgtcgacg aagaggtcct tctgcgcgct ggcggcggac ggcgcgagag tggctgcgac
     3421 cagtgtggcc gcgaaaagcg cacggcggac gttcattatc ggggcctcac gccgggccgg
     3481 atttggcgag gctacgataa cagacccaat taatcgccgc ttcacaaatc tgacgcgtcc
     3541 tttacaatct cagcgccgtg gggtgggtga cttgccgtct cccgttcgta ttcgccgccg
     3601 tcggcccggc tctgctcgtc tattcgacca tcccgctcgc gcaggagcgc cctctcagaa
     3661 tcatcgacgt cgcttcgcag tccggcatcg acctcctcaa tgtctccgga tctcccgcga
     3721 aggactacat cgtcgaggca aacgggaacg gcgttgcgtt cttcgactat gacaacgacg
     3781 gagacatgga tgcgctgctc gtcaacggct cgacgatcga ccgcgtggcc agaggcggcg
     3841 atccgatggt cgcgctgtac gaaaacgacg gccgagggcg attccgggac gtgaccagta
     3901 acaccggcct gaaccggcgc gggtggggaa ccggtgtatg catcgccgac gtcgacaacg
     3961 acgggttcca ggacctctat gtcacggccc tcggacccaa cgtgctgtgg cggaacaccg
     4021 gagagcgacg gttcctggcc accgaccagg cgtcggaccc gcgctggagc accggctgcg
     4081 cgttcggcga ctacgaccgc gacggcgacg tggacctgta cgtcgcgaac tacctggcat
     4141 tcgacgtcgc gaaggtgccg cgcaagggcg agacgtcgtg cggccgtttc atgaacatcg
     4201 tcgccctctg cggaccgcgt gggctcacac cgcagcccga cgcgctctac cgcaacaccg
     4261 gcgacgggca cttcgtcgac gtgacgcagg cggcaggcgt ggcccagccc ggccgcttcg
     4321 gtttcggcgt ggtgttcagc gatctcgacg acgacggctg gccggacatc tacgtcgcga
     4381 acgactcgat gccgaacttc ctcttccgca accgtcgcga cggaaccttc gtggaggagg
     4441 gcctgctgag gggcgtcgcg gtgagccggg acgggcgcga acaggccagc atgggagtcg
     4501 atgccgcgga ctacgacgga gacggcgact tcgacctcgt ggtcacgaac ttcgcgcagg
     4561 actacacgac cgtctaccac aacctcggcg gggggacttt cgcggacagg agcttcgagt
     4621 ccggcgtcgg ggcgtacggc ccctccctcg gctggggtgt tggctttgtc gacatcgaca
     4681 acgacggcct gctcgatctg ttcgtctcca acgggcacgt gtacccggac gtcgagcaga
     4741 ccggcaccag tacctatcgc caacgaagcc tgctctttcg aaaccagggg agggggtacc
     4801 tcaggaaggt gacgaccagc gagggtgggt tggcgctcgc caggtcgagc cgcggggccg
     4861 cgttcggcga ctacgacaac gacggcgaca tcgacgtatt gatttcgaac atcgacgatc
     4921 gcccgacgct cttgcgcaac gacaccagcg gcggaagctg gatcacgatg cgccttgtcg
     4981 gagtgaagag caatcgggac gggatcggcg caaaggtggc ggtggtcgcc ggcgggcggc
     5041 gacagactgc cgaggtccgc gccggcggca gctacgtttc acacaacgac atgcgactgc
     5101 atttcggctt ggggaaccaa gaccgcgtcg acgtggtcga gattcggtgg ccgagcgggc
     5161 tagtggaacg cgtggacggg ctgcgggcga atcgcttcta ccaggcgcgg gaaggctcgg
     5221 gcatccaaca ggatcccttg ttgacacgat gattgcctcg cagcgtactg tgatggcgtt
     5281 gagacttctc ctgctggtct gtctgccggc gcttctcgcg actgacgcag aggctcaggc
     5341 gcagctccag ttcttcgcga ccatcgtcga ctcgagcggc gcgccgggga cggccgtaca
     5401 gcccggcaac atccaggtga cggagaacgg tgtcgaagcc aggctcgtga agatcgaaca
     5461 ggtcaactgg ccgctgaaag tgcagttgct ggtggacaac ggcgtcgggc tcggacgcga
     5521 gaacctcgtc tcgctgcgcg ccggcgtaag aggattcctg gaggcgttgg cccagggaac
     5581 cgaggtcacc ttcgtcacca cgtcgccaca gccgcgcttc ctcgtgcgcg ccacgaccga
     5641 tcgcgaagcg ctcctccagg ggcttggcca gttgacgccg gactcgggat cggggcgctt
     5701 cgttgaatcg ttgagcgagg cgacgcagcg gattgagcgc gacaagaccg acttctttcc
     5761 ggtgatcgtg tccgtcgcga caacgtcctc cgatgccaac gcgcgggaga gcatgatcca
     5821 gcagctgatg ggccggctcc agaagcgtcc gaccacggtg catgtcgtgc tgctgacgcg
     5881 ggccgcagga aggacggcga gcgacggata cattcagcac gagctcgggc tgaacgtcac
     5941 gaaattcacc ggcggacgtt tcgagaacat cggggcaggc gcacagctgg aaacgctgct
     6001 cgccgagatt ggcgctctcg tggccaagag caacccgggc ggcgcaagca agttccgaat
     6061 caccgtggag cgccccggcg gtgcaagcgg cgatctgggc aagatctcgg ttatcttgag
     6121 cggcggtttc gtcgtcaccg acctttcgct cacgtctcga tagctacgcc gtcggtctca
     6181 cttcccggtc gtctcgccgc gcagccgctc gttccgctcc cgcaggtcgt tctcgtactt
     6241 ctgccgccgc tcgtcgagga gcctctcctg cagacgctcg aactcggcga gctgctgcct
     6301 cccttcctcc tccttgtcga ggccaagcag tgttctcgag agggcgtagc gagccggtgc
     6361 ggagtccggc gcgatggcaa ccgcgcgccg gagcatcgtc tcggccatcg tcagacggcc
     6421 ggcattgagg tggatctgcc ccatcacggc gagtgtttcg gcgtcttcct gtccgaggtg
     6481 cgacgagacc agcagctcga ccagcgcctc cacgtcgcga cctgcccgat ggtaggcgag
     6541 ccccaggctc ctgtgggcga gcggattgtt ccgcatcagc cgggtctgtc gcgtgaaggc
     6601 ggagatgacg cgctcctcgt cgaggagttc gtgcgcgaaa ctggcgagcc gcaacaggag
     6661 cgccgtcttg cccgcgatca tcgtgagcgt ggcggcctgc tcgagttgat cgatcgcgtc
     6721 cgcgtcgcgg ccggtcaact cgtacagatc cgccagggcc cagcgggtac ggcccgacgc
     6781 cggcaggagc cgtagcgtgt cgagcagtac gcgctcggcc tcctcgatcc tgcccgcctc
     6841 catcaacacg cggcccatgg cgatgtgggc ccgctcgtcc gcgggggcca cgcggatcgc
     6901 ctccgccagc cacttcaggc ttgcgtcgac gttgcccgcc gtggcgtgcg caacgcccag
     6961 caggcgacga gcctcggagg acgacggagc agccgcgacg gccgcctcga gatcggccac
     7021 ggcgtcggtg gtctgaccct gccgcagcgc cgcgacaccg cgcgcggcgg gtccggacct
     7081 cgccacatgg tccacgacca gcggatcgcg gaccatcgca gcccgaaaca atccgatcgc
     7141 gtcctcgtaa cgcccgttcg ccagcgcggc cacgccgtcc gcgtatgcgg ctcgcgagaa
     7201 ttgcggcgcc tccgacgcgc tgtcgtcgat caacccaacc tggatgaacg acgctcgccg
     7261 tgtggaagtg cccacgcttg ggcgctgcgg cggcccggcc ggaggccgga ccgcctggcc
     7321 agtgctgtct ggtcgggtcg cgtcgcgacc cgacattggc gaaggcggct cggaaccgat
     7381 tgccgcgagg agcgatgcca actgacgctc gggcggctcc gacggccggg tggacaaggc
     7441 gtcggcgagc aggtaggcgt tgacgacgtc atccgggtca agcgcccacg cccgctggaa
     7501 ggacgcaagc gcgtccgcgt cacgacggtc cgcgccgagc agcacaccgc ggagcatgtg
     7561 cagcccggcg cgttcgggct tgagccggat ggcctcctcg acggcctcca gagcatcggc
     7621 cggtcgccct tcattgagga agaaccgtgc cagggctgaa gagtcgctgg cagcccgcag
     7681 cgacgcttcc gccaagcggc tctcagcgtt ccacgcctcg agcgccacgt ccatcctgtc
     7741 gagcgctgct tcgaccctgg ggccctcgtc gccgtaggtg cccgagagcg ccgaatgaaa
     7801 ctcgaggagt gcctcgatga acgcgccctt ctgcgccgcg gccggcgcgg gcagcaggag
     7861 gaggaaaacg agcgcacaga ccctgctcac gttccgacct cagcgctgaa accgtgtgct
     7921 ggcgacgatt cccgcgccct cgcgaatgac gatcacctgg ttggccgcca cgttctgcac
     7981 cacctccgtc tgcccgctcg gccaccggat ctccacgcgg tccacgattg tgccagcgcc
     8041 aagcccgacg tggaggcggg gatcgttctg gcccagatag ctcgatccgg acgtcacctc
     8101 gcggacctgc gtgcggccac ccgatgtcaa ccggacccgc gcgccgatcg catcgcggtt
     8161 gctcatcgtg cctgtggtga gcgaagtcga accaccgatg agccgcagca gcagcgcgtt
     8221 tccgccattg ccaccgtcgt tgagcaggag ctcggcctgc tggccgttgt tggtgacgag
     8281 gaggtccaga tccccgtcgt tgtcgacgtc gccccacacg acggtgcgcc cgactttttc
     8341 cacggcgaag ggcgggcccg acagcgggct cacgttgcgg aatctgccgg cgcccgtgtt
     8401 ctgcatgagc agtcgcttct gcgcgtgctt cgcgccgcta cgacgcatcg cgatgttgtc
     8461 gatcacgtgc ccgttgacga tcgccaggtc gaggtcgccg tcgttgtcgt agtcggcgaa
     8521 cgccacgccg aaccccacgt gctggagcgt ggccggcccg attccgctgc tcgcggtggc
     8581 gtccgtgaac gctccgttgc ccgcgttgcg gaagagactg tgcatctccg tctcgtggtt
     8641 ggtcacgacg agatccaggc gtccgtcgcc gtcgacgtcg ccgaacgccg tgcccatgcc
     8701 cgcgcgagcc ttgccgtccg aggcgaccgc cacgccggcc acgagcgcaa gctcggtgaa
     8761 ccgtccgtcc cccttgttgt ggaacaggaa gttcggcacg ccatcgttgg ccacgaacac
     8821 gtccggccac aggtcgtcgt catagtcggc gaccgcgacg cccaggccgt tgcccgtaaa
     8881 cgacgcgata ccggcgcccg ccgagacgtc ggtgaaggcg ccatcgccgg cgttgtggta
     8941 gagcacgctc ggcgacggcc ggaaattgag cgggtgacag tacattcgca gcttctccgg
     9001 gtgcgtgttg ccgcagaacg ggttctcctc ggcgccttgg acgtagttgg tgacgaacag
     9061 gtcgagccgg ccgtcgcgat cgacgtcggc gaaagcgcag ctcgtgctcc agagcggcga
     9121 tcccacgcgg gcggtcgtcg tgatgtcggt gaatgcgccg ttcccccggt tgcgatacag
     9181 aacgttgggg ccgtcgttgg tggtgacgta gagatcggtc aggccgtcgt tgtcgtagtc
     9241 gcccgcgcag gcgcccatgc catacgcgag atgccggatg ccggagttgg cagtcacgtc
     9301 ctcgaaggag ccgttccccc ggttgtgaaa gagccggtgg cgcgcggtgc ccgagacccg
     9361 gggatccgct ttggaacccc cgtcgaccag gaatacgtcg atccagccat cgttgtcgaa
     9421 gtcgaagaag agccctcccg agcccatgat ctcggccagg tgcttgtccg ggctcgcgcc
     9481 gttgacgtgc gtgaagacaa gcccggacct cttgctgacg tcgtcgaaag ccacccgttt
     9541 cgaggcgtcc ggccactgcc ccgcggtgcc ggagatggcc agcgtcgtga aggccagtcc
     9601 aggcaggacg gccacccagc gcggcatgac caacaccatt tagcagattc ctcgcaattc
     9661 tcaaaccttc tggtagtggt ctagttcaaa accactacca atcttcttga cacgcttgag
     9721 ctcaggagcg tatacccaca atgccaatcc ggggctctag ccccggatga gcggaggagt
     9781 ggaggagtcc gttgatgcta caaggcgcga aagtgacact cgtacttgcc gtgttcgtga
     9841 tgcttctgcc ggcatcagcc cttgcccagc aaggcaatat cgcagggacg gtccgcgaca
     9901 gttccggagc cgtcctgccc ggtgtggtcg tggaagcgac gagccctgcc ctcatcgaga
     9961 aagtgcgttc aggtgtcacc gacgagaccg ggcaataccg catcaccaat ctgcctgtcg
    10021 gcacctattc ggtcgccttc cggctcgaag ggttctccac ggtggagcgc gcgaacgtcg
    10081 tgctgagcac cgggttcacc gcgccggtca acgtgacgat ggcggtcggg caggtcgccg
    10141 agaccgtcaa agtggtggcc gagacgccga ccgtcgacgt gcagagcgcg cgtcaggtcg
    10201 ccagcttcac gggcgcggac atccgcgatc tgccgacgac gcgcaacatc cgcagcatcc
    10261 tgacgatgac gccggggctc accccgtcgg gcctgggcgc cgactgcgtg ggcaacgtcg
    10321 gcgtctggtg caacaacaac atctacaacc tgggcgcgca caccgacgtc aacaccgcgg
    10381 gtgccttcga tcccccagac cagatcgccc aggacgggct gaaccagggc cgcatcatgg
    10441 tggacgggac gatcatcaac accggcggcg gcgcgggcat catggggatg accggcggct
    10501 acgtcgccga cgtcgccaac tcccaggaag tcaacgtgca gatctccgga gcgctgggcg
    10561 agtcggagac cggcggggcg acgatcaaca tcgtgccgcg caccggcggc aacaactttg
    10621 ccggcaacta cttcgtgacc tacacgcggg gtccgctgga ctcggctggc aacggccgta
    10681 ccgggtggtt cagccagaac aacggcaact atcccgagat caataacaac tatccgctcg
    10741 tcagcgacgt cgacacgtcg gccgcgttcg gcggcccgat cgtgcgggac cacctgtggt
    10801 tcttctcggc cgggcggatg tggcagaaga acgccttcag ctcgcaggta gaccgcatct
    10861 gggacaacgc gaacgccggc atctatggcg agaactacga agcggattat tcgacagacc
    10921 cgctgcagct ggtcaactgg acgcgcaacg ccaacgtgcg cgtgacctgg caggcgacgc
    10981 ggaaggacaa ggtcaacttc ttctgggacg agggcttcac ctgccaggat ccgtgtgacg
    11041 ggtcggtggc ggcctggacg tcgcgggacg gctggtggtc gggacaggtg catcccgccc
    11101 ggctgatcca ggcgagctgg acgaatccgc tgaccaacac catcctggcc gaggcgggtc
    11161 tgtcggtgaa ccggcagctt tacgacttct cccagcatcg ctactggacg cccaatccgg
    11221 acattccgcg cgtgatcgaa cttggatcga ccgtcggcta taactacgcg cagaacgcgc
    11281 cgctcaacac cgtgaatttc aacctctttg gtgttccgtc gggaccgtgg tccgacggta
    11341 tcggcgggtt ggcggagata cggaacctca acgacttgcg gccgcgcgcg tcggtctcgt
    11401 atgtgaccgg cgggcacaac atgaagatcg gctacgacgg cggctacttc tcacagacca
    11461 ggcaaaacag aaccaacaac ccgcgcctgg agtaccgata cttcacaccg gcggcgacct
    11521 gctttgacgc cgcgaatccc gccgcgtcga cgtgcggcaa cacgagccgc tatcaccccg
    11581 aggacccgta caactcgctg cgccggccgg tgcccgacca ggtcaagatc agtaccgggc
    11641 cgtccgccat cgagaaccgc gtaggctata gcggcttcta tgcgcaggat cagtggacgc
    11701 tgaaacgctt cacgttgagc ggcgccatcc gctacgacca ttcgttcagc cgctaccccg
    11761 gcacgtgcat cggcgccgcc gccggcaacg accttgtcgc cggcgccagc ccggaaccct
    11821 acgtccccgt gcaggtgggc gggcagtatg gcgggcagcg ggagtactgc acgcccgaca
    11881 acgacggcgt ctcctaccac gacgtgaccc cgcggtgggg cgtgacgtgg gacctgttcg
    11941 gcaacggcaa gacgtcgatc aagtacaacc agggccagta cctgtcgggc gcggggatca
    12001 gcggcatcta ttccagcgcc aacccggtgg agcgcgggaa gaacacgtac acccgcacct
    12061 ggagcgacct ggacggcgac cgcttcccgg actgcgacct gctgaactac agcgcacaat
    12121 ccccggcgac tggggccgtg gacgcctgcg gcggaccgaa ctcggtcttc attttccccg
    12181 ccggctttga ggaccccacg cgatacgggc gcgacccgta cagcctggat gcgagcggcc
    12241 agttgctggg cctgcaaacg acctattgcg gcagcaccga gctcgccatc ccggcggccg
    12301 tccagtccta ctgcaacgcc tacggcgaca cgctgatcga agggtggggc aggcgccgct
    12361 ccgagtggca gttcggtctc ggcatccagc acgagatcct gccgcgcctc tcggccgagg
    12421 tgacctacaa caggcggtcc ttcgcgaacc agacggtgaa cgacgccctt gggctcggct
    12481 gcgatcggtt caatagcaca tacacactcg aagagtgcaa tgacctctac ctgaaccaca
    12541 cccacccgga ctacggcttc tacactgtca cggcgccctc taatccggac ctgccgagtg
    12601 gcggcggcta cgtggtgcga ggcctggcgg acccgaacgc gctcaggttt ccagggccga
    12661 cagcggtgac gatcctgcaa acgctggagt actcctggaa cggcgtggac accaacttcg
    12721 tgtggcgggc gccgggcggg ctgcgcgtca acggcggcac cagcaccggt cgctctgtgc
    12781 gtgacctgtg ccacgccgcg actgacgccc cgaacgtcaa ggtgtacgac ggcgcggcgg
    12841 tggcctgcaa cccgcaccgc cggtgggaca cgaacgtccg cggcaccgcc gcgtacacga
    12901 ttcccaagat cgatatgctc gtgagctcgg tgttccagtg gcggccgggt gtagagcggg
    12961 cggcgaacta caccttcaac aagaatcagc tgacgtggga tgcgtccagc gccttccggg
    13021 cgacccagcc ctgcgccaac cctgccaacg gcacggggtg cctgacgtcg ggaaacgccg
    13081 gctccggcgg gacgaccacc accgtgaatc tcctcaacac cggcgaggtc tacggcgagc
    13141 cctactcgat tgtcgacctg aagatcgcga agaacatcaa gttcgccaac aagcgcctca
    13201 atgtcggcgt cgacatctac aacctcttca acgacgatgc gatcaggagt taccaggaca
    13261 ccctcgacac ggttgacaac cccaacacgc cggtggtcga gaagtatggg caggcgacct
    13321 cactgctgag tccgcgcttc gtgcgtctgt cggtccagtt cgacttctaa tctcgtcggt
    13381 cgttcgtgac ttggcgcccg cgcattcttc ggagtgcgcg ggcttttttt ctgtgcgtgc
    13441 accccttccc tttgaacccc ttgagggcga tcctcgatgc cgggccatga ccgcagtgcg
    13501 gacacccgga ccgggcactt gtagacttac ttacacgctt tgacctttgc acttcgaact
    13561 ctcaccgtct gcgttctgtg tctcctcggc gcggcgtttc caggcgccgc cgactacgct
    13621 atccgcctcg tggacgtgcc gggcggtggc ggtatcaacc tcctcaacgt ctcgggctct
    13681 ccgaccaagg actacatcgt cgagggaaat gggaacggcg ccggcttctt cgactacgac
    13741 cgcgacggag acctcgacgc gattgtcatt aacggctcga cgttcgaccg tctcaagtcg
    13801 ggcggcgatc cgctgacggc cctgtacagg aacgacggcg gcggccgatt cagcgacgtg
    13861 accgcatcga gcggcatgaa ccgccgaggc tggggcagcg gcgtctgcgt ggccgactac
    13921 gacaacgacg gcttcgagga cgtgtacatc accgcgttcg ggcccgacgt gctctaccgc
    13981 aacacgggac gcggcgcctt caccgccacg aaccaggcga gcgacgagct ctggagcacg
    14041 ggctgcgcct ttggcgacta cgatcgtgac ggccacctgg atctgtacgt ggcgaactac
    14101 gtggcgttcg accgcgcgaa ggttcccagg cgcggcgccg gcggctgcaa gttcatgaac
    14161 atcgacgctt tctgcggacc acgcccgttg cccggggccc gcgacgtgct ctatcggaac
    14221 cagggagacg ggacgtttgt cgatgcgacg gcgaaggcaa ggacgatcga gcccggccac
    14281 tacgggttcg gcgttctctt cagcgatctg aacgacgacg ggtggcccga catctacgtg
    14341 gccaacgact cgctccccaa cctgctgttc cggaaccggg gcgacggaac cttctcggaa
    14401 gaagcgctgc tcgccggcgt ggccgtgagc ggggacggac gcgagcaggc aggaatgggt
    14461 gtcgatgccg gcgactacga cgccgacggg cggctcgatc tcatcgtcac gcacttcgcc
    14521 caggactaca ccacgctcta ccgcaacgag ggcgacgggc tcttccggga cgtgagcttc
    14581 gagtccggca tggccgcgac gctcggtctt ccgctcggct ggggagtggg gctgctggat
    14641 ctcgacaaca acggctggct cgacgtgttc cagtcaaacg ggcatatcca ccccgagatc
    14701 gcgaagacgg gcaccagcac ctatcggcag cggaaccagg tgtttcgcaa tatcgggggc
    14761 aagcgcttcc agcacgtgac cgacgatgcg gggaacgcgc tgcgggtcga gaaatcgtat
    14821 cggggcgccg cctttggcga ctacgacaac gacggcgatc tcgacgtgct cgtcatcccg
    14881 atcgacgatc gcccgacgct ccttcgtaat gacacggcgg ggggccactg gatcacgttg
    14941 cggctcgtcg gcagcaaaag caaccgcgac ggcatcgggg ccagggtgac gatcgaggcg
    15001 ggcgggcgtc gtcagatcgc cgaggtgcgc agtggcggca gctacgtgtc gcacaacgac
    15061 atgcgggtcc atttcggact cggcgaggcg cgccgcgtcg atcgactcgc gatccgctgg
    15121 ccgagcggcc tggtcgaaac agccaccggg ctcgacggcg atcggttcta cgtggcgcgg
    15181 gaggggtcgg ccgtccgcgc ggggctctga cacatcagat tccgcacagt gtttcgccgg
    15241 cgcagtggaa aagagctggc ctgcaatagt ttgaacccat tagctcgtcc ttgacttcct
    15301 ggcttctgca tagacaatta aggcgtactc gcagagggaa cagtatttcg ctctgcgaag
    15361 agctaggtca aagtggcccg ggttgagccc tgtctgggag gaaattgatg caacgagctg
    15421 tgaaagtgat cctcgcgctc gcagccttcg tcgttctgct gccgggggcg tcgttcgcgc
    15481 aggaaggtca gatcgccggg acggtccggg actcgtccgg cgcggtcatg ccaggggttc
    15541 tcgtggaagc cacgagcccc gccctcattg agaaggtccg cgccaccacc agcgacgcga
    15601 gcgggcagta tcgcctcacc aacctgccgg tcggcaccta ctccatcacc ttcacgctcg
    15661 aagggttcaa taaatggcag cgcgagatga tcgtgttgac cagcggattc accgcgtcca
    15721 tcaacgcgac catggccgtc ggccaactcg cagaaacggt gacggtgctc agcgagactc
    15781 ctaccgttga cgtgcagagc gcgcggcagg ccctcacctt ggccggcacc gaccttgccg
    15841 aactgcccac caaccgcaac gtcaacagcc tgctggcgct cacgccgggc ctgacgagcg
    15901 gctacgctcc cggcacgttg tccggcatct gcagcggcgg tgtgggcgtg ttctgcaacc
    15961 ccggcgtcac cggcttcaac gtgggcgaca acgacccgac gaacatggcg caaggccggg
    16021 tcctggtgga cggccaggtg atcaacagcg gcaccgcggt tccgatcgtc ggacagacgg
    16081 gcggatacac ggccgacatc gccaacgccc aggaggtggc catccagatc tcgggcgcca
    16141 tgggcgagtc cgagacgggc ggcgcgtcga tcaacatcgt tccgcgcacg ggcggcaacc
    16201 ggttcgcggg caacttcttc acgtcgttca ccgacgactc gttcttttca cagaacaacg
    16261 gtgcctaccc gggcatccct gcggtgtttc agccggtcat ctcggatcac gacatcatcg
    16321 gctccttcgg cggacccatc aagcgcgaca cgctctggtt ctactcggtg gcgcgcaagc
    16381 agggcataca caaactgccc gtaggagtcg acttctggcc gaacttgtgg gaagggcagt
    16441 ggggctacaa ctatcagccc gaccgtgccg agccgcgcgt cgagtacaag aacatctggc
    16501 gcaacgtgaa cgcgcgcgtc acctatcagg cgacgcaaaa gaacaagttc aacttcttct
    16561 gggacgagca ggacttctgt caggacccgt gcctcggcgt cgtgtcggtc ttcacctcgc
    16621 ctgagtcgtg gtggtcggtg cagtcctacc cgaaccgcct gcagcaggcc tcgtggacaa
    16681 acccgctgac caacaagatc ctgctcgaag cggggatcaa catcacatcg cagcagtaca
    16741 acacgacgga gcaccgcgag tacaccaacc cgcgcgcgat cccgcgggtc atcgagaccg
    16801 gcgaaacagc gggcggcgac gccaccgcgc cgcgcgtgaa ccagtttgca ggcgccgggt
    16861 tttttgccct cacatcgggg tcgctgaact cgcagatcaa cggcgggggc gccgagcgca
    16921 gggatctcga caactaccgg atgcgggcgt cggcgtctta cgtgaccggc acgcacaacg
    16981 cgaagttcgg ctatgacggc ggcttctact cgcagatcca gacgaacgag gcaaacgacg
    17041 tccggatgac ctacaactac gtctggccgg ccgccgattg cgccaacacc aacagctgcg
    17101 gcaacacgag tcttcagttc ccgaacaacc ccaccaacac gccgctcggt cccgacggcg
    17161 tcgccggcac ggctgatgac ggccggcggc cggtcccgag caacatcgtg ttcaatacgg
    17221 gcgtcgggag cctgaacgac aaggtgaact acacggccct gtacgcgcag gatcagtgga
    17281 cgctgaagcg gctcacgctg aacggcgctg tgcggttcga tcacgcggcg agcagctacg
    17341 gcgagacctg cgtcggcccg gaccggtacg tccccattca gacgaacggg gcgaacttct
    17401 actgcacgcc gccctccgat ggcgtgagct tcaacgacgt cacgccgcgc atgggcgtcg
    17461 tctgggacgt cttcggcacg ggccggacct ccgtcaagtg gaacatgggc aagtacctga
    17521 cggccgcggg catcagcggc atctactcca acgccaaccc ggcgcgacgc acggtgaaca
    17581 cactgcagcg gagttggaac gacatcgacg gcgatcgcct cgttgactgc gacgtgctga
    17641 acccggccat tacgccggag tgcggcgcgt ttctcttccc caacaacgac ctcacgcgat
    17701 tcggccgcga cccgctcggc ctcgatgcgg caggcacccc catcggcctg gcaaccacgc
    17761 agtgcggcag gcaggaggcc gggattccgg cggctgttca ggcctactgc gcggcctacg
    17821 gcgagtcgat gatcgagggc tggggccggc ggcagtcgga gtggcagttc ggcatcggcg
    17881 tacagcacga gctcctcccc aggctgtctg ccgaagtggt ctacaaccgc cggagctact
    17941 tcaacctgac cacgtcggac cagttgggcg tcgggtgcga tcgcttcaac ggcgcgcagg
    18001 acgttcgtac gtgccagcag gggaacctcg actacaccaa cccgtcatac gacttctaca
    18061 tggtcaatgt gcccagcgat tcgcgtctgc ccgacggcgg cggataccag gtcatcggcc
    18121 tcaacacgcc gaagacgtcg gtcccgtttg gtgcgccgac ggcccagacg ctgaaccccg
    18181 atctcgatta cgtctggaac ggcgtggacg tgaacttcgt ctggcgcgga ccgcagggcc
    18241 ttcgggtcaa cggcggcact cagactgggc gctcgaaccg cgacacctgc ttcgccaacc
    18301 tggatgctcc ggcggtgtcg ggacgcccgg gcgccgagta caagggcggc gagactccgg
    18361 ccggcccctt caacgttcct cccgcgcaac ccaacggctg ccagagcgtc acgccctggc
    18421 tgaccacggt caagggctcg gccgcctacg tcgtaccgtg ggtggatgtg ctcgtcagca
    18481 cgatcttcca gtcgttcccc ggcgtggagc gcaacgcggt catgaccttc ggcaagaacg
    18541 acgtgatctg gatgccgggc agcgagagtc gcgccacccg cccgtgcgcg aacccgctcg
    18601 acggcgtcgg atgccttggt cccaccggaa cccagacgac caccttcgtc aacctgctca
    18661 acaacaacga gatgtacggc gagcgggtga cctcgttcga tttcaaggcg tccaagatca
    18721 tccggttcgg gaacaggcgc gtgacgatcg gaggagacct gtacaacgtg ttcaactccg
    18781 acgcggtcca ga
//
LOCUS       DBNGSH010000056         3216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3909223, whole genome shotgun sequence.
ACCESSION   DBNGSH010000056 DBNGSH010000000
VERSION     DBNGSH010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3909223"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /gene="amrS"
                     /locus_tag="AC16CY_01195"
     CDS             complement(<1..990)
                     /gene="amrS"
                     /locus_tag="AC16CY_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR3352418.1"
                     /translation="MTLQEILAARTREAAPELVEVLERNRLRCYSCGHECPIPDGASG
                     VCKVRVNRGGRLHVPFGYVGGVQCDPIEKKPFFHVHPGALAYSIGMLGCDLHCSYCQN
                     WVTSQALRDPRAVAPPQDVTPAALADEAVALGARIVVSTYNEPLITAEWAVAIFTEAR
                     ARGLLTGFVSNGNGTERVLTYLRPYIDVYKVDLKSFNDRRYRELGGRLQPILETIAWL
                     HRSGVWVEIVTLLIPGFNDSDEELRGLTAFLAGVSLDIPWHVTAFHPDYKMTDVAATT
                     AAMLQRAAHIGQDAGLRYVYAGNLPGRVGDLEDTCCHQCGERLVVRRGYAIQQY"
     gene            1062..1814
                     /gene="surE"
                     /locus_tag="AC16CY_01200"
     CDS             1062..1814
                     /gene="surE"
                     /locus_tag="AC16CY_01200"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3352419.1"
                     /translation="MPRILVTNDDGVHSEGIKALADALRPLGEVVVVAPIQEASAIGH
                     ALTLRRPLRIETIGPDVYAVDGTPTDCVNLGITHVLKAKPDLIVSGINKGWNLGDDVT
                     YSGTVSGALEGALLEIPSIAVSAQRIGDDCEFGPSAAAAASAAQAVLERGLPKFTLLN
                     INVPHGPNKGFRVTVQARRNHVTVVSERMDPRQREYYWIEEGENEWEPHDRSDYQAVR
                     DGYISITPLQPDMTAYEALIYVEELQLQTTRS"
     gene            complement(1811..2065)
                     /locus_tag="AC16CY_01205"
     CDS             complement(1811..2065)
                     /locus_tag="AC16CY_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3352420.1"
                     /translation="MPIYEYACRTCGQEFEQLIRTGDTPACPSCKGQDLERLLSLSSV
                     SSEHTRKLSFDRARARAKLVQRDKDVAQAEYEKKHREEGH"
     gene            2104..2180
                     /locus_tag="AC16CY_01210"
     tRNA            2104..2180
                     /locus_tag="AC16CY_01210"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2138..2140,aa:Pro,seq:cgg)
     gene            complement(2186..>3216)
                     /gene="selD"
                     /locus_tag="AC16CY_01215"
     CDS             complement(2186..>3216)
                     /gene="selD"
                     /locus_tag="AC16CY_01215"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - L-selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR3352421.1"
                     /translation="PLTSLSHGGGCGCKLAPGVLEQILETAGADRLVPRELLVGLGTR
                     DDAAVYQINGEQAIVATTDFFMPIVDDPFDFGAIAATNAISDVYAMGGRPLFALALVA
                     MPIGKLPVETIRRILDGGESACRRAGIPVAGGHSVDSVEPIYGLVVIGLIDPRRVRRN
                     ADARPGDALVLGKPLGVGIYGAAVKKQTVTGAQYRELVATTTQLNTAGMPLAALDGVH
                     ALTDVTGFGLLGHLLEVCRGSGVRATLKWNQVPLLTDAHALVEAGCVTGGSARNWQGY
                     SGLVDLTTHGAIEQALLTDPQTSGGLLVACAPESVADVLDVFRAEGFARAAVIGRVDA
                     GAPGVVVI"
BASE COUNT          567 a         1061 c         1066 g          522 t
ORIGIN      
        1 gtactgctga atcgcgtagc cacggcggac gaccagtctc tcgccacact ggtgacaaca
       61 cgtgtcctcg agatcgccga cccggcccgg aaggttgccg gcatagacgt agcggaggcc
      121 tgcatcctgc ccgatgtgcg cggcgcgctg gagcatcgct gcggtcgtgg ccgccacgtc
      181 cgtcatcttg taatcgggat ggaacgcggt aacgtgccac gggatgtcga gcgacacgcc
      241 agccaggaac gccgtcaagc cgcggagttc ctcatcgctg tcgttgaacc cgggtatgag
      301 gagcgtgacg atctcgaccc agaccccgga gcggtgcagc cacgcgatcg tctccaggat
      361 cggctgcaga cgtccgccca gctcacgata ccggcggtcg ttgaagctct tgaggtcgac
      421 cttgtacacg tcgatgtacg gacgcaggta cgtgaggacg cgctcggtgc cgttgccgtt
      481 cgagacgaaa ccggtgagca agcctcgcgc gcgtgcctcc gtgaagatcg cgacagccca
      541 ttcggccgtg atcagcggct cgttgtaggt ggacaccacg atgcgcgcgc cgagcgcgac
      601 ggcctcgtcg gcgagcgctg ccggtgtgac gtcctggggc ggggccacgg ctcgcggatc
      661 gcgaagcgcc tgcgacgtca cccagttctg gcagtagctg cagtgcagat cgcagcccag
      721 catgccgatg ctgtaggcga gcgcgcccgg gtgcacgtga aagaacggct tcttctcgat
      781 cggatcgcac tgcacgccgc cgacgtaccc gaacggcacg tggaggcgtc cgcctcggtt
      841 gacgcggacc ttgcacacgc cggaggcgcc gtctgggatc ggacactcgt gtccgcagga
      901 gtagcagcgg aggcgattgc gctcgaggac ttcgaccagc tcgggcgccg cctcacgggt
      961 acgcgcagcg aggatctcct gcagcgtcat acttcgaact actagcggac tgtatccact
     1021 ttacactttc cacttctcac ttgctaagat cgtgactcct catgccgcga attctcgtga
     1081 caaatgatga tggtgtgcat tcggaaggca tcaaggcgct ggctgacgcg ctccggcctc
     1141 tcggggaggt cgtcgtcgtg gcgccgatcc aggaagccag cgccatcggc cacgcgctga
     1201 cgctgcgcag gccgctgcgc atcgaaacca tcggaccgga cgtgtacgcc gttgacggca
     1261 cgccgaccga ctgtgtcaac ctcggcatca cgcacgtgct caaggccaag cccgacctga
     1321 tcgtgtcggg gatcaacaag ggatggaacc ttggcgacga cgtgacgtac tcaggcaccg
     1381 tgtcgggcgc gctcgaggga gcgctcctcg agattccgag catcgcggtc tcggctcagc
     1441 ggattggaga cgactgcgag ttcgggccgt cggcggcggc cgccgccagc gccgcgcagg
     1501 ccgtgctcga gcgcggtctt ccgaagttca cgctgctcaa catcaacgtg ccgcacgggc
     1561 cgaacaaggg ctttcgcgtg acggtgcagg ccaggcggaa ccacgtcacc gtcgtgagcg
     1621 agcggatgga tccgcggcag cgcgagtact actggatcga agaaggagag aacgagtggg
     1681 agccgcacga ccggtccgat taccaggccg tgcgtgatgg ctacatctcg atcacgcctt
     1741 tgcaaccgga catgacggcg tacgaagcgc tcatatacgt cgaggaactg cagctccaga
     1801 cgacgcgatc ctagtggccc tcttccctgt gcttcttttc gtactccgcc tgcgcgacgt
     1861 ccttgtcgcg ctgcaccagc ttggccctgg cccgcgctct atcgaacgag agcttccgcg
     1921 tgtgctccga actgaccgag gacagcgaga ggagtcgctc gagatcctgt cccttgcacg
     1981 acgggcacgc cggagtgtcg ccggtccgaa tgagctgctc gaactcctgt ccgcaggtcc
     2041 ggcaggcgta ctcgtagatt ggcatggcag taacgtaccc ccatgttaag ctaggacctt
     2101 cgccggggcg tggctcagcc tggtagagcg tccggttcgg gaccggaagg tcgctggttc
     2161 gaatccagtc gccccgacca ttccttcaga tcaccacgac gccgggcgca ccggcgtcca
     2221 cgcggccaat gacggcagcg cgagcaaagc cttccgcgcg gaacacatcc aggacgtcgg
     2281 ccaccgactc gggcgcgcag gccacgagca acccgccgct cgtctgcgga tcggtgagaa
     2341 gcgcctgctc gatggctccg tgcgtcgtca gatcgacgag cccggaatag ccctgccagt
     2401 tccgcgccga gccgccggtc acgcatcccg cctcgaccag cgcgtgtgca tcggtcagca
     2461 gcggcacctg gttccacttc agcgtcgcgc ggacgccgga tccgcggcag acctcgagca
     2521 ggtgaccgag gaggccgaag ccggtgacat cggtcaacgc gtggacgccg tcgagcgcgg
     2581 ccaagggcat cccggcggtg ttgagctgag tcgtcgttgc cacgagctcg cggtactggg
     2641 cgccagtgac cgtctgtttc ttcaccgccg ctccatagat ccccacaccg agcggcttgc
     2701 ccaacacgag cgcatcgccc gggcgcgcat cggcattgcg acgcacgcgc cgcggatcga
     2761 tcagcccgat gacgacgagc ccgtaaatcg gttcgaccga atcgacgctg tggccgccgg
     2821 caacgggaat gccggcgcga cggcacgccg attcgcctcc atcgagaatc cgccggattg
     2881 tttcgacggg caacttgccg atgggcatcg ccacgagcgc cagcgcgaac agcggacggc
     2941 cgcccatcgc atatacgtcc gagatggcgt tggtggcggc gatggcgccg aagtcgaacg
     3001 ggtcgtccac aatcggcatg aaaaagtccg tcgtggcgac gatcgcctgc tcgccgttga
     3061 tctggtagac ggcggcatcg tcgcgagtgc cgaggcccac gaggagctcg cgcggaacga
     3121 ggcgatcggc gccggccgtt tccaggatct gttccaggac gccgggcgcg agcttgcagc
     3181 cgcagccgcc gccgtgcgag aggctggtga gcggaa
//
LOCUS       DBNGSH010000057         3464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2550847, whole genome shotgun sequence.
ACCESSION   DBNGSH010000057 DBNGSH010000000
VERSION     DBNGSH010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2550847"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="AC16CY_01220"
     CDS             <1..1015
                     /locus_tag="AC16CY_01220"
                     /inference="COORDINATES: protein motif:HMM:NF017365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HIR3352422.1"
                     /translation="IRPIKTIDAHAGGQTLRLVVEGFPRPRGRTLSQQWEWLRKHGAS
                     LRSALVRAPRGHDDMTAAILCESASPEAHAGILFMDGEGCPSTCGHGVITAATIAIER
                     ALFFTSQPDESGTRLVFETPAGIVRADARLAVHGETRRVDSVSTTNVPAFVHEASCPV
                     RIGSRELRVDIAFGGAFYAIVDTEAAGIPLTPARLPDLRRLGRDICAAANATARPDHP
                     ADASIRDVAGAIFTGPPHDPEAHLRNVTVTSGGLVDVSPNASGTSAVMAILDAMGLLP
                     EGQPFVHEGLSGSLVRGLVAGRTEVGDRAALITQVEGSAWITGEHTFFLDDEDPFREG
                     INF"
     gene            1159..1338
                     /locus_tag="AC16CY_01225"
     CDS             1159..1338
                     /locus_tag="AC16CY_01225"
                     /inference="COORDINATES: protein motif:HMM:NF017451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3352423.1"
                     /translation="MAGFRSHEEIIAWQRANELKLLVYDLIESGPVTRDADLHDQLRR
                     SARAAPRLIAEGFGR"
     gene            complement(1518..2777)
                     /locus_tag="AC16CY_01230"
     CDS             complement(1518..2777)
                     /locus_tag="AC16CY_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR3352424.1"
                     /translation="MRRKSKRQPSEALLAPVPSEILDQFVRQGPITPEELDAAVRRFK
                     KAIIERALGGELTHHLGYPPGGSKPEETTNHRNGTGPKTVLTDDGPLSIAVPRDREGT
                     FEPQLIPKHERRFTGFDDKILALYARGMTVREIQAFLAEMYEVEVSPDLISTVTDGIV
                     AEVTAWQSRPLERVYPVVFFDALRVKIRDEATVRSKAIYLALAILPDGTRGILGLWIE
                     QTEGAKFWMKVFADLKARGCQDILIAVTDGLKGMSEALAAVFPATTLQTCIVHLIRHS
                     LDFGNWKERKPLAMALRPIYTAPHADAAAAALDDFERGPWGKKFPTVVTSWRRAWTHV
                     IPFFAFPPDVRRLIYTTNSLESVHARLRKIIKTRGHFPTDEAAIKLLWLALRNITAKW
                     ERVAYYWRPAMNQFAILYEDRFPLPNT"
     gene            3191..>3464
                     /locus_tag="AC16CY_01235"
     CDS             3191..>3464
                     /locus_tag="AC16CY_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352425.1"
                     /translation="MRHRVLPVLLVVCWVSSAEAHPAPFSYLDIVFKSGGIEGTLVVH
                     VIDVAHELGIPPERLMDEALVAAERQRIGEILAPRLELRGGERLTIE"
BASE COUNT          605 a         1096 c         1144 g          619 t
ORIGIN      
        1 gatcagaccc atcaagacca tcgacgctca tgcggggggc cagaccttgc gcctggtggt
       61 cgagggcttt ccgcgtccgc gaggcaggac gctgtcgcag cagtgggagt ggctgaggaa
      121 gcacggcgcc tctcttcgat ccgcgctggt ccgggccccg cgcgggcacg acgacatgac
      181 ggcggcgatc ctgtgtgaat cggcctcacc cgaggcgcac gccggcattc tgttcatgga
      241 cggggaaggc tgcccctcga cctgcgggca cggcgtcatc accgccgcga ccatcgcgat
      301 cgagcgcgcg ctgttcttca cgagccagcc ggacgagtcg ggcactcgcc tggtgttcga
      361 gacgccggcc ggcatcgtgc gcgccgacgc caggctggcc gtccacggcg agacgcgccg
      421 agtcgattca gtgtcgacga ccaacgtgcc cgcgttcgtg cacgaggcgt cgtgcccggt
      481 caggatcgga tcgcgggagc ttcgcgtgga cattgcgttc gggggagcgt tctacgcgat
      541 cgtggacacc gaagccgccg gcattccgct cacgccggcg agactgcccg acctccgccg
      601 gctcgggcgg gacatctgcg cggcggccaa cgcaacggcg cggcccgatc atcccgccga
      661 tgcgtcgatc cgcgacgtcg ccggcgcgat cttcacgggc ccgccgcacg atcccgaggc
      721 gcacctccgc aacgtgaccg tcaccagcgg cggattggtg gacgtgtcgc cgaatgccag
      781 cggcacctcc gcagtgatgg cgattctcga cgccatgggc ctgctgcccg aggggcagcc
      841 cttcgtgcac gaaggattga gcgggtctct cgtgcgcggt ctcgtcgcgg gccgtaccga
      901 ggtcggcgat cgcgccgccc tgatcacgca ggtcgaaggg tccgcgtgga tcaccggcga
      961 gcacaccttc ttcctcgacg acgaagatcc ctttcgagag gggatcaatt tctgagtact
     1021 tgggttcagg gggttcacaa gggttcaatg ggttcaaggg gtcctgttca gggggttctg
     1081 gaggttcttt aggttcaatg ggttcggggt tcaaaggttc cactcccgag cgggcatgcg
     1141 gcgtgcacga accgaggcgt ggccggattt cgttctcacg aagagatcat cgcctggcag
     1201 cgtgccaatg agctgaagct gctcgtctac gacttgatcg agtcgggtcc agtgacgcgt
     1261 gacgccgacc tgcacgacca gcttcgtcgc tcggccaggg cggcgccgcg actcatcgca
     1321 gaggggttcg gacggtaggg agtgtcagga atttcgtgtg tgaggcgtga ttgacggttt
     1381 ggtcttctct tctctcctga caaacgaatg aggcgtgtgg agcgctgtgg gaaaccgcgt
     1441 cctgtgcggt tttccaagtc gctgtgggcg cgttctgtgc gtccacggcg acggcggcgt
     1501 ccacgcgcgg tcacatttta cgtgttcggc agcggaaagc gatcctcata gagaatcgcg
     1561 aattgattca tggccggtcg ccagtaatag gcaacccgct cccacttcgc cgtgatgttc
     1621 cgcagcgcga gccagagtaa tttgatggcg gcctcatcgg tcggaaaatg gccgcgcgtc
     1681 ttgatgatct tgcggagccg cgcgtgcaca ctctcgagtg aattggtcgt gtagatcagc
     1741 cggcgtacgt cgggcgggaa ggcaaagaac ggaatcacgt gggtccacgc ccggcgccac
     1801 gaggtgacca ccgtgggaaa tttcttcccc caggggccac gttcgaagtc gtcgagcgcc
     1861 gcagcggcgg cgtccgcgtg gggcgccgtg tagatcggtc gcagggccat ggcgagcggc
     1921 ttccgttctt tccaattgcc gaagtccagg ctgtggcgaa tgaggtggac gatgcaggtc
     1981 tggagggtcg tggcgggaaa gacggctgcc agcgcctcgc tcatgccttt caggccgtcg
     2041 gtcacggcga tcaggatgtc ctggcagccg cgggctttca gatcggcaaa gaccttcatc
     2101 caaaacttgg cgccctcggt ctgctcgatc cagaggccga ggatgccgcg ggtgccgtcg
     2161 ggcagaatcg ccagcgcgag gtagatggct ttgctgcgga cggtcgcttc atcgcggatc
     2221 ttgacccgca gcgcatcgaa aaagaccacc ggatagacgc gctcgagggg gcgactctgc
     2281 cacgccgtca cctcggccac gatgccgtcg gtgactgtgc tgatcagatc cggggagacc
     2341 tcgacctcgt acatctcggc gaggaacgcc tggatctcgc gcaccgtcat cccgcgcgcg
     2401 tagagcgcga gaatcttgtc gtcgaatccc gtaaaccggc gctcgtgctt cggaatgagc
     2461 tgcggctcga acgtgccctc gcgatcgcgc ggcacggcaa tcgacagtgg accgtcgtcg
     2521 gtcaggaccg tcttgggtcc cgtcccattg cggtgattgg tggtctcctc gggtttgctc
     2581 ccgccaggcg ggtagcccaa atggtgcgtc agctcaccgc ccagcgcgcg ctcaatgatc
     2641 gccttcttga accggcggac cgctgcatcg agctcctcgg gcgtgatcgg cccttggcgg
     2701 acaaactggt cgagaatttc agacgggaca ggcgccaaca gcgcctcgga aggctggcgt
     2761 ttcgacttgc ggcgcataga cactcctcat gctatgcccc acacacgaaa ttcctgacac
     2821 cctcggacgg tatcttcccg gcgatttcag ccgttacctg cgcagtgcga atggcgagct
     2881 caaagaaacg tacgacgcgc tgcgggacgg ggtcgatcga aagtacttca cctcaactca
     2941 gatcgagccg atgctgcgcc tgtcaaagcg cgcctcgaag gctgcgacga agttgatcgc
     3001 gtaccttcga accgccgaag cgcccaacga agaacgtcgt ggcagatcga ctcgtcgccg
     3061 acgcgacgcc gatgcctcaa gaaccagatg aacctcgaac ccctgaaccc atagaacccc
     3121 ggaacccatt gaacaaaacc cacggaacct acggaacccc ctgaacccat tgaaccagta
     3181 gaatccagga gtgcgccaca gggttcttcc cgtcctcctg gtcgtgtgtt gggtgagctc
     3241 ggccgaggcc catccagcgc ccttcagtta cctcgacatc gtgttcaaga gcggcggcat
     3301 cgaaggcacg ctcgtcgtgc acgtgatcga cgtcgcccac gagctcggga taccgcccga
     3361 gcgcctgatg gacgaagcgc tggtcgcggc cgagcggcag cgcatcggcg agatcctcgc
     3421 gccgcggctc gagctgcgcg gcggcgagcg cctgacgatc gaat
//
LOCUS       DBNGSH010000058        10442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1192367, whole genome shotgun sequence.
ACCESSION   DBNGSH010000058 DBNGSH010000000
VERSION     DBNGSH010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1192367"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="AC16CY_01240"
     CDS             complement(<1..548)
                     /locus_tag="AC16CY_01240"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302377.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR3352426.1"
                     /translation="MPAQSIPDLIPFPRVILIVLDSVGCGELPDAASYGDAGSNTLGN
                     IAARVPLRIPHLRSLGLGRLVELGAPSVDHPRAAYGRMAEASAGKDSVTGHWEMMGIV
                     LDRPFPTYPSGFPREVIEAFERRIGRGTLGNVAASGTEIIDRLGAEHLRTGKPIVYTS
                     ADSVFQIAAHEDVIPLSEQYRI"
     gene            complement(526..1467)
                     /gene="miaA"
                     /locus_tag="AC16CY_01245"
     CDS             complement(526..1467)
                     /gene="miaA"
                     /locus_tag="AC16CY_01245"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR3352427.1"
                     /translation="MAKVLAVLGPTASGKSALGLALASCCNGEIINCDSTAVYRGFDI
                     GTDKLSAEERRGIPHHLIDIADPTESYTAARFANDAEQAIRAIHGRGKLPILVGGTGF
                     YYRALTRGLFPGPGADPELRARLDRVAARKGPERLHRLVQSVDPASARRIMPRDRKRL
                     VRALEVYYATGQPLTAHFEQTASLIADCEVIAVALRLPAAATAERVARRVDEQFARGV
                     VAEVQALLSRGVPPDARPFGGLVYRQVMEMLRGVRDEAATRQLIAMENRRYARRQLIW
                     FRKEPNLIWFDGPGERPETLQRVREDLLARRACAEHS"
     gene            complement(1654..2604)
                     /locus_tag="AC16CY_01250"
     CDS             complement(1654..2604)
                     /locus_tag="AC16CY_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR3352428.1"
                     /translation="MIHFDFDDRYQDELIVGNAISVRDGVLLSLIVHAVIIALFQFGP
                     RLHLFDPSPEELEARRQELQRRLEEESAERRFVFVQPRVDLEALRPPPRAPLSDADRE
                     AQARERALKPQNPLPFARGNSPERAEANQPERARGVETPVKPDTQPPAPEPEEQVAEA
                     LPPADLGVRRSPTISRPAPGALGDALKNLERYVQNETFNNPQGGVTDPGATIQFDTKG
                     VEFGPWLRRFVAQVRRNWFVPYAAMTFHGRVVLQFNIHKDGRITDLTVVSPSSINAFN
                     LAAHNAISGSNPTEPLPTEYPEPKAFFTVTFYYNENPPLN"
     gene            complement(2692..3435)
                     /gene="ubiE"
                     /locus_tag="AC16CY_01255"
     CDS             complement(2692..3435)
                     /gene="ubiE"
                     /locus_tag="AC16CY_01255"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIR3352429.1"
                     /translation="MISVADQAPDKSPDRIASMFDAIAPRYDLLNHVLSAGIDRRWRR
                     RMIASLRLTGRETLVDVCTGTADVALEARRGAGTGPSGTAARVLGVDFAAAMLALGLQ
                     KVRSAGEDRRIVLVRGDALRIPAGDSCADAATVAFGVRNVAEAGVACRELARVLRYGG
                     RLAILEFGVPALPGLRAIYRWYFSNVLPAIGRFVSGHQGAYSYLPASVGAFPPPAEFV
                     ALLRQSGFGQVVAVPLTFGVVYLYTAVRE"
     gene            complement(3445..4296)
                     /locus_tag="AC16CY_01260"
     CDS             complement(3445..4296)
                     /locus_tag="AC16CY_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HIR3352430.1"
                     /translation="MNKLTTYFSFVRFSHSVFALPFALTGALLAWRENPFSWSQVAWI
                     VVCMVGARSAAMGFNRLVDARLDALNARTAMRELPQGRMSRREAVSFVVVSSAVFVYA
                     ASRLSTLCLLLSPVALAIVFWYSLAKRYTTYTQMFLGLALAVAPVGGWIAAGGRGGAE
                     PWLLGLAIGLWVGGFDILYACQDVDFDRAHGLRSIPATFGVRNAIRLSRVMHAVTVLA
                     MAALWWAASLPELYLVGVAVVAVLLVYEQSLVSDDDLSQVKRAFDLNGWVGIVYFVVT
                     ALALYVG"
     gene            complement(4293..5522)
                     /locus_tag="AC16CY_01265"
     CDS             complement(4293..5522)
                     /locus_tag="AC16CY_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3352431.1"
                     /translation="MLTFRAQWIVPIAGEPIRNGFVTVDSNLRIQDVSTTGSEHAPWL
                     GRVAVLPALVNAHTHIELSFLQGRVPAAATFNDWVTTLMSVRRASTADPFASPILDAA
                     RHAIEQAKATGTGLVGDVSNSLATVPLLREARLSAHVFHELTGFNLPDVPGRIAEARR
                     AVDAARAGADDVRIGLAPHAPYSVSPELFKAIRSDVDAHPHPVTTVHLGESAQEVQFL
                     RDGTGPARAMLERLGVWTDAWRPPGGSPVEYLAGLGFLDARTLVVHGVQFGGDDLARL
                     KTIGATLVSCPRSNVHVGAGSPPLEAFYASGVDVAIGTDSLASAPDLNMFNELAEARR
                     IAPRVPARRLLESATLTGARALGFDRDFGSIEVGKYASFAVVTMPPRRVTDVEEFLVS
                     EVEPGAIRRSGFPPATS"
     gene            complement(5543..6745)
                     /gene="mqnC"
                     /locus_tag="AC16CY_01270"
     CDS             complement(5543..6745)
                     /gene="mqnC"
                     /locus_tag="AC16CY_01270"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HIR3352432.1"
                     /translation="MIDRIASKVMDGGRVSAAEALELYREAPTHLLGRLADSIRQRRH
                     PDGIVTYIIDRNVNYTNVCVARCNFCAFYRPVGSSEGYVLGFAEIFEKIDETIAVGGN
                     QLLLQGGHNPDLPIAWYEDLFRAVKGRYPDFKLHALSPPEVLHISRLNQLPVPAVIDR
                     LVAAGLDSIPGGGAEILVDRVRRLLNCYTKASSDEWIDVMRHAHKKGLRTTATMMYGT
                     VETDEERIEHLMRLRDLQDETGGFTAFITWSYQPEHTEHGGFEETGIDYLRTLALSRI
                     VLDNFDNLQASWVTQGGKVGQLSLAYGANDMGSVMIEENVVRAAGAAYCMDELEIVVN
                     IEDAGFVPKRRNMHYDVLGDPIFRERDVPRMLALATARADGDTSVPDELRNYEARSRA
                     GKTLRLTQ"
     gene            complement(6762..7553)
                     /locus_tag="AC16CY_01275"
     CDS             complement(6762..7553)
                     /locus_tag="AC16CY_01275"
                     /EC_number="4.2.1.151"
                     /inference="COORDINATES: protein motif:HMM:NF014658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthetic enzyme MqnA/MqnD family
                     protein"
                     /protein_id="HIR3352433.1"
                     /translation="MTHVRIGAVGYLNARPLTWALDRDPGRWRVRYDVPSVCSALLHR
                     GEVDLGLIPSVEYLQSSDYRFVPGIGVCSRGAVASVALYTRRPIEDVRHVALDSSSRT
                     SVALIQILCRHRFRITPSFVPHGPDLAAMTVACDAALLIGDPAFDAPHEALGLRKIDL
                     GEEWTAMTGLPFVYAAWTGRPGAADKGVVSALQRAQVDGLAATDAIASEYGRGDAARA
                     ARAAAYLRDNVKYELGADEASGLQLFLDYAADLGVAPRRRTVEFF"
     gene            complement(7553..8446)
                     /locus_tag="AC16CY_01280"
     CDS             complement(7553..8446)
                     /locus_tag="AC16CY_01280"
                     /inference="COORDINATES: protein motif:HMM:NF039584.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352434.1"
                     /translation="MPSATTFDRYHERVGAGESLSPDEVRDLAVAPDILSIGMLADVV
                     RRQRHGARTTFLRVAEGPFDRTSFDGDQPAAREVRLTGSPPDIAAAVSAVESVKAAAG
                     DRLVSGFSWSDVERWSSGERPVQVLKQLRAAGLEAIADLPLDGLTDPDAAVESLLGAG
                     FLQLRLAISKSPAAERLDLLLRARDLQARHGCIQSVNPLPNVLATFRPTTGYEDVKMV
                     ALARLAAPAVPSIQVDWRRYGPKLAQVALTFGADDIDGVSGSDAAPEGRRRAPLEEIR
                     RNIQAAGLIPVERDGRFAALS"
     gene            complement(8457..9395)
                     /locus_tag="AC16CY_01285"
     CDS             complement(8457..9395)
                     /locus_tag="AC16CY_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3352435.1"
                     /translation="MSAPLRVAVIGVGHLGQHHARLLASMEGVHLVGVVDIKDGRAAQ
                     VGGALGVKAFTHSGELPPESIDAVTIAVPTVSHADVALPFIDRGVSVLVEKPLASSLP
                     EADRLIDAAARRGVVLAAGHTERFNPAVVAALPLVSNPRFVEVHRLGTFPERSLDIDV
                     VFDLMIHDLDLLLTAVKSEVVSVEAVGVNVLTPRVDIANARLRFASGCIANVTASRIS
                     RDRVRKVRFFQRDSYLSIDYAAQEVEVYRLVAENGRPEIQGGNLEVRRDEPLRAELAD
                     FVEAVRERRAPVVSGQAGRDALELAAKVAALMESAT"
     gene            complement(9392..10225)
                     /locus_tag="AC16CY_01290"
     CDS             complement(9392..10225)
                     /locus_tag="AC16CY_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HIR3352436.1"
                     /translation="MTLGLIAGNGRFPFLVLDAARAMGHDVTIVAVKEEAFPELADAA
                     RRHATPIHWISLGQLGTCIRLLAEAGATRAVMAGQVKHTKIFGGVVPDMTLISVLRHL
                     ASRNTDGLIGAVAGVLREHGIELIDSTSLLTPLLARPGVLTDRAPSDDERRDFEFGYR
                     MADAIAGLDIGQTIAVKHLAVVAVEAMEGTDEVIGRAGHLAGPGVRIVKVAKPKQDMR
                     FDVPVIGFATIQAMRVAGATALSIDAGKTLVLDGDAVYASANEAGITMVGRETSGPVD
                     A"
     gene            complement(10246..>10442)
                     /locus_tag="AC16CY_01295"
     CDS             complement(10246..>10442)
                     /locus_tag="AC16CY_01295"
                     /inference="COORDINATES: protein motif:HMM:NF025097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352437.1"
                     /translation="QLRHAYRYLLQSKLNTTRALAQIEQDTSLSTPEVRYLVDFIRSS
                     QRGVILRKPTRRAEELVADE"
BASE COUNT         1803 a         3579 c         3461 g         1599 t
ORIGIN      
        1 catatccggt actgctccga cagcggaatc acgtcctcgt gcgcggcgat ctgaaacacg
       61 ctgtcagccg aggtatagac gatgggcttg cccgtgcgaa gatgctcggc gcccagccga
      121 tcgatgatct cggtgccgga cgccgccacg tttccgagtg tgccccggcc gatcctcctt
      181 tcgaacgcct cgatgacttc gcgcggaaac ccggagggat acgtgggaaa cggccgatcc
      241 agcacgatgc ccatcatctc ccagtgtccg gtcaccgagt ccttgcccgc cgaggcctcg
      301 gccatgcggc cgtaggccgc gcgcgggtga tcgacggacg gcgcacccag ttcgacgagg
      361 cgaccaaggc cgagcgaccg gagatgcggg atgcgcagcg ggactcgagc ggcgatattg
      421 ccaagcgtgt tgctgcccgc gtcgccgtaa gacgcggcgt ccggcaactc gccgcatccc
      481 acgctgtcga ggacgatcag gatgacgcgc ggaaaaggaa tcaggtcagg aatgctctgc
      541 gcaggcacgg cgcgctaaga ggtcttcgcg aacgcgttgc agcgtctccg gccgttcgcc
      601 gggtccatcg aaccagataa ggttaggctc tttgcggaac cagatcaact gccgcctggc
      661 gtagcgccgg ttctccatcg cgatcagctg tcgcgtcgcc gcctcgtccc gcacgcctcg
      721 caacatctcc atcacctgcc tgtagaccag accgccgaaa gggcgtgcgt ccgggggaac
      781 gcctcgcgac agcaacgcct gcacttccgc cacgacgcca cgcgcaaact gctcgtccac
      841 tctgcgcgcg acccgctctg ccgtcgccgc cgcaggcagc cgcagcgcca cggcgatgac
      901 ctcgcagtcc gcgatgagcg aagccgtctg ctcgaaatgc gcggtgagcg gctggccggt
      961 cgcgtaatac acctcgagag cccggacgag ccgcttccgg tcgcggggca tgatgcgcct
     1021 ggccgacgcc ggatcgaccg actgcacaag acggtgcagg cgctcgggcc cctttcgcgc
     1081 cgccaccctg tcgagccgcg cgcgcagttc ggggtcggcg ccaggacccg ggaagagccc
     1141 gcgagtcagt gctcggtagt agaagccggt tcctccaacc agaatcggca gctttccgcg
     1201 cccgtggatg gcccggatcg cctgttccgc gtcgttggcg aaccgagcgg ccgtatacga
     1261 ctcggtggga tcggcgatat cgatgaggtg atgagggatt cctcgccgct cctcggcgga
     1321 cagcttgtcg gtgccgatgt cgaacccgcg gtacaccgcc gtcgagtcgc aattgatgat
     1381 ttcaccattg cagcaggagg ccagcgccaa gcccagggcg ctcttgccgg acgcggtagg
     1441 accgaggacg gcgaggacct tggccaacga tcaggcgagc ctgatgaaga cgtacacgat
     1501 cagcgccgcg aagagcagga tcagcccgag ctgttgagat ctcgttggga gtcgggggtt
     1561 gggccgcctc cgcgccgaag gcgcttcggc gagccgcgcc ggagccttgg cgttggcggg
     1621 cgttgggagt tgactggatc tgtccactgc tcttcagttc agcggcggat tctcgttgta
     1681 gtagaacgtg accgtgaaaa acgccttggg ctccggatac tccgtcggga gcggctcggt
     1741 cgggttcgag ccggagatcg cgttgtgcgc cgcgaggttg aacgcgttga tgctggacgg
     1801 cgacacgacg gtcaggtcgg tgatccgccc gtccttgtgg atgttgaact gcagcacgac
     1861 gcgtccgtgg aacgtcatgg cggcataggg cacgaaccag tttcgtcgca cctgtgcgac
     1921 gaatcggcgc agccacggcc cgaattccac accctttgtg tcgaactgaa tcgtcgcgcc
     1981 cggatcggtg acgccccctt gtggattgtt gaaggtctcg ttctgtacgt agcgctccag
     2041 gttcttgagc gcgtcgccga gcgctcccgg ggccggacgg gatatcgtcg gggaacgacg
     2101 gacgccaaga tcggcgggcg ggagcgcttc ggccacctgc tcctccggct ccggggcggg
     2161 gggttgggtg tcgggcttca ccggagtctc cacgccgcgt gcgcgttccg gctggttggc
     2221 ctcggcccgt tccggcgagt ttccgcgcgc aaacggcagc gggttctgcg gcttgagtgc
     2281 gcgctcgcgg gcctgtgcct cccggtccgc atccgacaag ggggcgcgag gcggcgggcg
     2341 caacgcctcg aggtccactc tcggctgcac gaagacgaac cgccgttccg cgctctcttc
     2401 ctccaaccgg cgctggagct cctgccggcg tgcctccagt tcctcagggc ttggatcgaa
     2461 cagatgcagg cggggaccaa actggaagag ggcgatgatc accgcgtgga cgataaggga
     2521 cagcagcacg ccgtcgcgta ccgaaatcgc gttgccgacg atgagctcgt cctggtaccg
     2581 gtcgtcgaag tcgaaatgta tcaagagatt gaaagcaaat tatacctgtc gcctacaggc
     2641 tgacgcccac tgacctcgta tcggctggag gcggaaactg ctgggggcct gctattcccg
     2701 gacggccgta tataggtaga cgacgccgaa ggtcagcggg actgcaacga cctggccgaa
     2761 ccccgactgt cgcaagaggg ccacgaactc ggccggaggc ggaaaggcgc ctaccgacgc
     2821 cgggagatat gaatacgcac cctggtggcc ggacacgaac cggccaatcg ccggaagcac
     2881 gttggagaag taccaacggt agattgcccg gaggcccgga agggctggaa ccccgaattc
     2941 caggatggcc aggcggcccc cgtaacgaag cacccgggca agctcccgac atgccacgcc
     3001 ggcctccgcg acattgcgca caccgaaagc gacggtcgcg gcatcggcac atgagtcgcc
     3061 tgcgggaatc cgcagcgcgt ctccacgaac cagcacgata cggcggtcct cgcccgcgga
     3121 acgcaccttc tgcaggccga gtgcgagcat cgcggccgcg aaatcgacac ccagtacccg
     3181 ggccgccgtc cccgacggcc ccgtcccagc gcctcgacgc gcttcgagcg cgacgtccgc
     3241 cgtgccggtg cagacgtcca cgagcgtctc gcggcccgtg agccgaagcg aagcaatcat
     3301 ccggcggcgc cagcgtcgat cgattccggc gctcaggacg tgattgagga ggtcgtaccg
     3361 cggggcgatc gcatcgaaca tgcttgcgat gcggtcgggt gacttgtcgg gtgcctggtc
     3421 agccacggag atcacgatgg gtggctaccc gacatagagc gccagcgcgg tcaccacgaa
     3481 gtagacgatc ccgacccacc cgttgagatc gaaggccctc ttcacctgcg acaggtcgtc
     3541 gtcgcttacg agcgactgct cgtagacgag cagcacggcg acgaccgcga cgccaacgag
     3601 atagagctcg gggaggctgg ctgcccacca caacgccgcc atggccagca ccgtcacggc
     3661 gtgcatcacc cgcgagagcc tgatggcgtt tctgacgccg aacgtggcgg ggatcgacct
     3721 caggccgtgc gcgcgatcga agtcaacgtc ctggcaggcg tacaggatgt cgaacccgcc
     3781 aacccagaga ccgatggcga ggccgagcag ccatggctcg gcgcctccgc gccccccggc
     3841 agcaatccag ccgccgacag gcgccacggc gagcgcaagg cccagaaaca tctgcgtata
     3901 agtggtgtac cgcttggcca gcgaatacca gaacacgatc gcaagcgcga cgggcgacag
     3961 cagcaggcac agggtagaca accgcgacgc ggcgtagacg aacaccgccg aagacacgac
     4021 gacgaagctc acggcctccc ggcgcgacat cctgccctgc ggcagctcac gcatcgccgt
     4081 tcgcgcgttg agcgcgtcga gcctcgcatc gaccagccgg ttgaagccca tcgcggcgct
     4141 gcgcgcgcca accatgcaga cgacaatcca cgcgacctgc gaccaggaga acgggttctc
     4201 gcgccacgcc aggagcgccc cggtcaacgc aaacgggagc gcgaagaccg agtggctgaa
     4261 ccggacgaac gagaaatagg tcgtcagctt attcacgagg ttgccggcgg aaacccgctc
     4321 cttctgattg cccccggctc gacttcggag acgaggaatt cttccacatc tgtcacacgt
     4381 cttggcggca ttgtcaccac ggcgaacgac gcatacttgc cgacctcgat gctgccgaaa
     4441 tcgcgatcga agcccagggc acgggctccc gtgagcgtgg cgctctcgag cagccgccgc
     4501 gccggcacgc gcggtgcgat gcgccgcgcc tcggccagct cgttgaacat gttgagatcg
     4561 ggcgcgctcg caagactgtc ggtcccgatg gcgacatcga caccggaggc gtagaaggcc
     4621 tcgagtggag gtgagccggc gccgacgtgg acgttgcttc gtggacacga cacgagcgtc
     4681 gcgccgatgg tcttgagccg cgcgagatcg tccccgccga actgcacgcc atgcacgacc
     4741 agggttcggg cgtcgaggaa accgagaccc gcgaggtact ccaccggtga tccaccggga
     4801 ggccgccagg cgtccgtcca gacaccgagc cgttccagca tcgcgcgggc gggtcccgta
     4861 ccgtctcgaa ggaactggac ctcctgcgcc gactcgccca gatgaaccgt cgtcacgggg
     4921 tgcgggtgtg cgtccacgtc gcttcggatc gccttgaaca gctccggcga gacggaatat
     4981 ggggcgtgcg gcgcgagacc gatgcggaca tcatctgcgc cggcgcgtgc cgcgtccacg
     5041 gccctgcggg cttcagcgat ccggcctggc acgtccggca ggttgaaccc ggtcagctcg
     5101 tgaaagacgt gcgccgacag acgcgcctct ctgagcaggg gcacagtcgc gagcgaattg
     5161 ctcacgtcgc cgacaagtcc cgtccccgtg gccttcgcct gctcgatggc gtgccgcgcg
     5221 gcgtccagga tcggactcgc aaacggatcc gccgtcgatg cgcggcgcac cgacatcagc
     5281 gtcgtgaccc agtcgttgaa ggtggccgca gcaggaacgc ggccttgaag aaacgacagc
     5341 tcgatgtgcg tatgcgcgtt caccagcgcg ggcagcacgg caacccggcc gagccacggg
     5401 gcgtgctcgg agcccgtggt cgaaacgtct tgaatccgca agttgctgtc gacggtgacg
     5461 aatccgttgc gaatgggctc gcccgcgatc gggacgatcc actgggcacg gaacgtgagc
     5521 atcggctgtc ggcggcggtc ggttactgtg tgagacggag cgtcttgccc gcgcggctgc
     5581 gggcctcgta gttgcgcagt tcgtccggca cggaggtgtc gccgtcggcc cgcgccgtcg
     5641 cgagcgccag catgcgcggc acgtcccgct cgcggaagat gggatcgccg agaacgtcgt
     5701 agtgcatgtt gcggcgcttc gggacgaaac cggcgtcctc gatgttcacc acgatctcga
     5761 gctcgtccat gcagtaggcg gcacccgccg ctcggacgac gttctcctcg atcatcacgc
     5821 tgcccatgtc gttggcgccg taggcaaggc tcaactgccc cactttcccc ccctgggtca
     5881 cccacgaggc ctgcaggttg tcgaagttgt cgagcacgat ccgcgagagc gcgagagtgc
     5941 ggaggtagtc gatgccggtc tcctcgaacc cgccgtgctc ggtgtgctcc ggctgataac
     6001 tccaggtgat gaaggccgtg aaaccgccgg tctcgtcctg cagatcgcgc agccgcatca
     6061 ggtgctcgat ccgttcttca tcggtctcga ccgtcccgta catcatcgtg gccgtcgtcc
     6121 gcagcccctt cttgtgcgcg tggcgcatca cgtcgatcca ttcgtcggag gaggccttgg
     6181 tgtaacagtt caggagccgg cggacccggt ccacgaggat ctccgcgccg ccgccgggga
     6241 tgctgtcgag cccggccgcg acgagacggt cgatcaccgc cggcacgggt agttgattca
     6301 gccgcgagat gtgcagcacc tccgggggcg acagcgcgtg cagcttgaag tcgggatagc
     6361 ggcctttcac cgcgcggaag agatcctcgt accacgcaat cggcaggtcg gggttgtgac
     6421 ctccctgcag cagcagctgg ttgccgccca cggcaatcgt ctcgtcgatc ttctcgaaga
     6481 tttcggcgaa gccgagcacg tagccctcgc tcgagcccac cggccggtag aacgcgcaga
     6541 aattgcagcg cgctacgcag acgttggtgt agttgacgtt gcgatcgatg atgtaggtga
     6601 cgattccgtc cggatgccgg cgctgccgga tcgagtcggc gagccggccg agcagatgcg
     6661 tcggcgcctc ccgatacaac tccagcgctt cggccgctga cacacggccg ccgtccatca
     6721 cctttgaagc gatccgatcg atcatcaacc gctccgcgcc ctcagaagaa ctccaccgtg
     6781 cgccggcgcg gcgccacgcc caagtcggcc gcgtagtcga gaaagagctg gagtcccgac
     6841 gcttcgtccg cgccgagctc gtacttcaca ttatccctta ggtaggcggc ggccctcgcg
     6901 gcacgagcgg cgtcgccgcg gccgtactcc gacgcgatgg catcagttgc cgccaggcca
     6961 tccacctggg cgcgctgcag ggcgctcacc acgcccttgt ctgccgcacc aggccggccg
     7021 gtccacgccg cgtagacgaa cggcagcccc gtcatcgccg tccactcctc gccgagatcg
     7081 atcttgcgca ggccgagcgc ctcgtgcggc gcatcgaacg ccggatcgcc gatcagcagc
     7141 gccgcgtcgc acgccacggt catcgcggcc aggtccgggc catgcggcac gaagctgggg
     7201 gtgatgcgga agcgatggcg gcacaggatc tgaatcagcg cgaccgacgt gcgcgagctg
     7261 gagtcgagtg ccacgtgccg gacgtcctcg atcggccggc gcgtgtacag cgccaccgac
     7321 gcgaccgcgc ctcgcgagca gacgccgatc cccgggacga accggtagtc ggacgactgc
     7381 aggtactcga ccgacggaat caacccgaga tccacctcgc cacggtgcag cagcgccgaa
     7441 cacaccgacg gcacgtcgta acgaacgcgc cagcgtccgg gatcgcgatc gagcgcccag
     7501 gtgagcgggc gggcgttgag gtaccccacc gcgccgatgc ggacgtgcgt catcagctca
     7561 gcgccgcgaa ccggccgtcg cgctcgaccg gaatcaggcc cgccgcctgg atattgcgcc
     7621 tgatctcctc gagcggcgcc cgccgtcgtc cttcgggtgc ggcgtccgat ccggacacgc
     7681 cgtcgatgtc gtcggctccg aacgtcagcg ccacctgggc gagcttcgga ccataccggc
     7741 gccagtccac ctggatcgaa gggaccgccg gcgcagccag cctggcaagg gccaccatct
     7801 tcacgtcctc atagccggtc gtcggccgga acgtcgcgag cacgttcggc agcgggttca
     7861 cggactggat gcagccgtgt cgagcctgca agtcgcgcgc cctgagcaga aggtcgaggc
     7921 gctcggccgc cggagacttg ctgatcgcga ggcgcagctg caggaatccg gcaccgagca
     7981 ggctctcgac ggcggcatcc gggtctgtca gtccgtcgag cggaagatcg gcgattgcct
     8041 cgagccccgc ggccctgagt tgcttcagga cctgtacggg gcgttcgccg ctcgaccatc
     8101 gctcgacgtc gctccaggag aatccagaca cgaggcgatc accggccgca gccttgaccg
     8161 actccacagc gctgaccgcc gccgcaatgt ccggcggaga cccggtcagg cggacctcgc
     8221 gcgccgcggg ctggtcaccg tcgaatgaag tgcggtcaaa cggcccttcg gccacgcgca
     8281 ggaacgtcgt ccgcgcgccg tgccgctgac gcctcacgac gtcggcgagc atgccgatcg
     8341 acaggatgtc aggcgccacc gcgagatcgc ggacctcgtc cggcgacagc gactctccgg
     8401 cgccgacgcg ctcgtgatac cggtcgaatg tggtggcact gggcatgtgc atctgcttac
     8461 gtggcggact ccatcagcgc cgccaccttc gccgccagct cgagggcgtc gcgccccgcc
     8521 tgtccgctga caacgggagc gcgccgctcg cggacagcct cgacgaaatc ggccagctcg
     8581 gcgcgcaatg gctcgtcgcg ccgcacctcc aggttgcctc cctgaatctc cggccgcccg
     8641 ttctcggcaa cgagccgata gacctcgacc tcctgcgccg cgtaatcgat cgacagatag
     8701 gagtcgcgct gaaagaagcg taccttgcga acgcgatccc ggctgatgcg gctcgccgtc
     8761 acgttcgcga tacagcccga cgcaaagcgg aggcgtgcat tggcgatatc gacccggggc
     8821 gtgagcacgt tgacgcccac ggcttcaacc gacaccacct cggacttcac cgccgtcagc
     8881 aggaggtcga ggtcgtgaat catcaggtcg aacacgacgt cgatgtcgag acttcgttcg
     8941 ggaaacgtgc cgagccggtg cacctcgacg aaccgcgggt tcgacacgag cggcagcgct
     9001 gcgacgacgg ccggattgaa gcgctcggta tgcccggcgg cgaggacgac gccccggcgc
     9061 gcggcggcgt cgatcagacg gtccgcctcc ggcagcgacg acgccagcgg cttctccacc
     9121 agcactgaca cgcctcgatc gataaatggc agcgcgacgt cggcgtgcga cacggtcggt
     9181 acggcgatcg tcacggcatc gatcgactcc ggcggcagct cgccggagtg ggtaaacgcc
     9241 ttcacgccga gcgcgccgcc gacctgcgcg gcccggccat ccttgatgtc gaccacgccg
     9301 accaggtgga ccccctccat cgacgccagc agccgcgcgt gatgctgccc gagatggccg
     9361 acgccgatga cggcgacgcg cagcggcgca ctcatgcgtc caccgggccg gacgtctcgc
     9421 gcccgaccat cgtgatgccg gcctcgttcg ccgacgcgta cacggcatcg ccgtcgagca
     9481 ccagcgtctt gcccgcgtcg atcgacagcg ccgtcgcccc tgccacccgc atcgcttgaa
     9541 tcgtggcgaa gccgatcacg ggcacgtcga accgcatgtc ctgcttcggc ttggccacct
     9601 tgacgatgcg gacaccgggg cccgcgaggt gccccgcgcg gccgatcact tcatcggtgc
     9661 cctccatcgc ctcgacggcc accacggcca ggtgcttgac ggcaattgtc tggccgatgt
     9721 cgagacccgc aatggcgtcc gccatccggt atccgaactc gaagtcgcgg cgttcgtcgt
     9781 cggacggcgc gcggtcagtg agcacgcccg gcctggccag cagcggcgtc agcagcgatg
     9841 tcgagtcgat gagctcgatg ccgtgctccc gcagcactcc cgccaccgcg ccgatcaagc
     9901 cgtccgtgtt cctggaggcg agatgcctga gcaccgagat gagggtcatg tctggaacga
     9961 cgccgccgaa aatcttcgtg tgcttcacct ggccggccat cactgctcgc gtggcaccgg
    10021 cctccgcgag cagtcggatg cacgttccca gctgtcccag cgagatccag tgaatcggag
    10081 tcgcgtgccg ccgggcggcg tcggcgagct cgggaaaggc ttcctctttc accgccacga
    10141 tcgtcacgtc gtgccccatg gcgcgtgcgg catcgaggac gaggaacgga aacctgccgt
    10201 tgccggcgat caggccgagc gtcacctcac cgctcctcga cgacgttatt catctgcgac
    10261 gagctcctcg gcccggcggg tcggcttgcg cagaatgacc ccgcgctgcg agctacggat
    10321 gaagtcgacg aggtagcgca cctccggcgt cgagagcgac gtatcctgct cgatctgcgc
    10381 gagcgcccgc gtggtgttca gcttcgactg gaggaggtag cgataggcgt ggcgaagctg
    10441 cg
//
LOCUS       DBNGSH010000059         6490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_343581, whole genome shotgun sequence.
ACCESSION   DBNGSH010000059 DBNGSH010000000
VERSION     DBNGSH010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_343581"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1697)
                     /locus_tag="AC16CY_01300"
     CDS             complement(273..1697)
                     /locus_tag="AC16CY_01300"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR3352438.1"
                     /translation="MTFEWSMPLVLEGATFALAVLALIAGLVRKGDSGRRIGWMTFIG
                     LLIVLGLTWVAEEGASLFEGAFVQDGLALFAKRLVLAAAALSVLVSVTSSQSTFVRRA
                     AEYHFTLLFSIVGMMVLVSARELVLLFVAFELMSIPLYVLTGFLKRDPAAPEAALKFF
                     LVGTVSAAVILYGMSFMYGVMGTTMLDGVPAAMAEGHPLMLLGMGLVLAGLGFKIAAF
                     PFHMWAPDTYEAGSTPFIAWLSVAPKAAGFVVIIRLYVEGGGGASLAWVPLISMLAAV
                     TIVAGNLMAIPQRNIKRLLAYSGVAHIGYMLIGLAAMSVTGVAMVLFYLVAYVFGNMG
                     AFLVVEAVARSEQSDDIDAYRGLAQRSPLLALAMLLFLLSLGGIPFVAGFWAKLYVFL
                     AGVERGLLGLVFLGAVLTVVALYYYLLVARRMYIEAPERHSQVPMPGLLGTAIAVCAL
                     GVVGIGVYPGPWVDAARRIAATLF"
     gene            complement(1694..3226)
                     /locus_tag="AC16CY_01305"
     CDS             complement(1694..3226)
                     /locus_tag="AC16CY_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR3352439.1"
                     /translation="MNAFPVLSVMTWAPFVAALAVMFLARDRPLLVRWLSLAGTTVSL
                     VASIWVYWAYDGSAAGFQFREEIALVPSVGISYLVAIDGMSALMALLTSIIIFAGVFA
                     SWTVKERSQEFYALLLILVTGVYGVFVSLDLFVFFLFYEIAVLPMYLLIGIWGSSGEV
                     RPQGIFGWAFGRTGVGTKEYAAMKLTLYLLFGSAFILVGILALFVAGGSQSFSLLSLQ
                     TMQFDPAMQTWVFLAFYIGFGILAGIWPLHTWSPDGHASAPTAVSMLHAGVLMKLGAY
                     GVVRVGMGLLPQATVDWAWLVGTIACINIVYGALSAMAQRDLKYVIAYSSVSHMGVVM
                     LGAATLTSNGLNGSVFQMFAHGIMTGLFFALVGLIYEKAHSREIFKMGGFGSMMPGIA
                     TAFTIGGLSSLGLPATAGFVAEFLTFLGAWASAHPWWLFPGVVGAFLTSIYVLRVAGQ
                     IFWGPKTEDPHFQHLPDAQGPEWAALGILVFVLVLFGVAPGIAIGPVDTATAPLLERL
                     GR"
     gene            complement(3223..5091)
                     /gene="nuoL"
                     /locus_tag="AC16CY_01310"
     CDS             complement(3223..5091)
                     /gene="nuoL"
                     /locus_tag="AC16CY_01310"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3352440.1"
                     /translation="MADPAYLSLASLLLPAASFLILAIVAPLRRSGRPAAYLSILFAL
                     GSLAGAVAAWRGQGEAVTRLVWDWLPAQGQPLATIGVLVDGSSTVMLALVALVALLVQ
                     VYSLGYLHEEPPPALGRYYTYQSLFAFSMMGLVLAPNFVQLFICWELVGLCSYLLIGY
                     WYKKPEAARAALKAFWTTKAGDVGLLIGIVLLWQQSGTFDFGEMRLMVADGRLPVAGL
                     GLITFCIYLGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVTAGVYLLHRTAWLF
                     ALTPEVLIVVAWIGAFTALLAAVLACVQDDIKRVLAYSTVSQLGYMMAAIGAGFSAAG
                     FFHLLTHGLFKALLFLGAGAVIHAVHSNDLPRMGGLARRMPQTAIVFLIGTLSLAGIP
                     FFGGFLSKEEILGAVWAGGLRVPFVALIFVAFLTAFYMFRVVFLAFFGPPRAGHDDRH
                     DQTGHSGAHEHDPPASMLVPLWILALLSMAVGVYFTFVHGEPEFSSPGWLTPVAIGAA
                     VGGIALALLTYQLGTIDPASLASLAGPIRRAALAKFWIDDLFEGVFGAATLGFSRLVG
                     WIDRYLVDGVLNVVSAWTVTTGDEMRTIQTGRAQDYVYGLGVGLLVLLLWVRWALV"
     gene            complement(5091..5402)
                     /gene="nuoK"
                     /locus_tag="AC16CY_01315"
     CDS             complement(5091..5402)
                     /gene="nuoK"
                     /locus_tag="AC16CY_01315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR3352441.1"
                     /translation="MSDYMGLQAYLTLAAVVFGIGLFGVVTRRNTIGILLGIELMLNA
                     VNINLVAFARFSGSEVGMIFTVFAICITVSEAAMGLAIVILLFRIRRTVMADHLDLLK
                     G"
     gene            complement(5469..5960)
                     /locus_tag="AC16CY_01320"
     CDS             complement(5469..5960)
                     /locus_tag="AC16CY_01320"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR3352442.1"
                     /translation="MAETLAFWSLAVVLVGSALAVVLSKNLFHSVLWLALALTGTAGV
                     FLLLESEFLAAVQLLLYAGGIVTIVVFAIVVTERLVGERLSQTSRSLAAGAVMAAAFV
                     AVVVNAIGQRPLPAERPPMAADLTRTLGESVLTRFVLPFELLALLMLAAMLGAVYFAR
                     PED"
     gene            complement(5960..>6490)
                     /locus_tag="AC16CY_01325"
     CDS             complement(5960..>6490)
                     /locus_tag="AC16CY_01325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoI/complex I 23 kDa subunit family protein"
                     /protein_id="HIR3352443.1"
                     /translation="AVLRAIGRAMWVTLVNLFRKPVTVHYPDVTRAYPDRYRGLLALV
                     YEKDTGEEACIGCRLCEYVCPPAVIKVEMLKSEKRNYAKTFTLELYACEFCELCVQVC
                     PTDAIVMTKSFDMPTADRREMLLDKDRLHALGLQHQVSWATGNRLREMQGPPKMARAE
                     AAHAKTDTAPPPGGAA"
BASE COUNT         1292 a         2219 c         2175 g          804 t
ORIGIN      
        1 ggagtagacg ccgtccgtga tcgatcgcaa ctgccgggcg tctccggcca gggtgctcag
       61 tatgaccagc gcgcagacac ccggcgcgat gcggagtgcg caccgaaacg ccgccgggcc
      121 cggttgaatg gagcgcacgt gaaaccctcc aactcgcggg aggcgcccgg attataacgt
      181 gcgccgcgag gtcccgctgc cttcgactcc gctcaggcca ggaagccgga caccacgcct
      241 gtcacgggtc cggctgaagc cggacactcc gtctagaaca gcgtcgcggc gatgcggcgg
      301 gccgcgtcca cccagggccc ggggtagacg ccgatgccga cgaccccgag cgcgcagacg
      361 gcgatggccg tgccgagcag gcccggcatc gggacctgcg agtgacgctc gggcgcttcg
      421 atatacatgc gtcgggcgac gagcaggtag tagtagagcg ccacgaccgt gagcacggcg
      481 ccgaggaaca cgaggcccag gagacctcgc tcgacgcccg cgaggaagac gtacagcttg
      541 gcccagaacc ccgccacgaa cggaatgcct ccgagcgaca gcaggaagag cagcatggcg
      601 agcgcgagca gcggcgagcg ctgggcgagg ccgcgatagg cgtcgatgtc gtcggactgc
      661 tccgaccgcg ccaccgcttc cacgacgagg aacgccccca tgttgccgaa gacgtacgcg
      721 accagataga agagcaccat ggccacgccg gtcaccgaca tggccgcgag cccgatgagc
      781 atgtagccga tgtgcgcgac gccggaatag gcgagcagcc gcttgatgtt ccgctgcggg
      841 atggccatca ggttccccgc cacgatcgtc acggcggcga gcatcgagat gagcggcacc
      901 caggcgagcg acgcgccgcc tccgccttcg acgtagaggc ggatgatcac cacgaacccg
      961 gcggccttcg gcgccaccga aagccacgcg atgaacggcg tgctgccggc ttcgtaggtg
     1021 tccggcgccc acatgtggaa ggggaacgcc gcgatcttga agccgaggcc ggcgagcacc
     1081 aggcccatgc cgaggagcat cagcggatgg ccctcggcca ttgccgcggg cacgccgtcg
     1141 agcatggtcg ttcccatcac gccgtacatg aacgacatgc cgtagaggat cacggcggcc
     1201 gacacggttc cgacgaggaa gaacttgagc gccgcttcgg gcgccgccgg gtcacgcttc
     1261 agaaagcccg tgagcacgta gagcggaatc gacatcagct cgaaggccac gaacagcagc
     1321 accagctccc tcgccgacac caggaccatc atcccgacga tcgagaacag caacgtgaag
     1381 tgatactcgg ccgcccggcg gacgaaggtg ctctggctgc tggtcaccga gacgagcacg
     1441 ctgagggccg cggccgcgag cacgaggcgc ttggcgaaca gcgccagccc gtcctgtacg
     1501 aacgcgccct cgaagagtga cgcgccttcc tcggccaccc acgtcaagcc gagtacgatc
     1561 agcaggccga tgaaggtcat ccagccgatc ctgcggcccg aatcgccttt gcgcacgagg
     1621 ccggcgatca acgccaggac cgccagcgca aacgtcgcgc cctcgagcac gagcggcatg
     1681 ctccactcga acgtcatcgt cccagccgct ccagcagcgg cgcggttgcc gtgtccaccg
     1741 gaccgatcgc gatgccgggc gccacgccga acagcacgag cacgaacacg aggatgccga
     1801 gcgcggccca ttcgggtccc tgggcatccg gcagatgctg gaagtgcggg tcctcggtct
     1861 tgggtcccca gaagatctgg cccgcgacgc gcagcacgta gatcgacgtc aggaacgcgc
     1921 cgacgacgcc cgggaacagc caccagggat gggccgacgc ccacgcgccg aggaaggtca
     1981 ggaactcggc cacgaagccc gccgtggccg gcaggcccag cgacgacagg ccgccgatgg
     2041 tgaacgcggt cgcgatgccc ggcatcatcg agccgaagcc gcccatcttg aagatctcgc
     2101 gcgagtgcgc cttctcgtag atgaggccca ccagcgcgaa gaagaggccg gtcatgatcc
     2161 cgtgcgcgaa catctggaat accgagccgt tcagcccgtt ggaggtgagc gtcgccgccc
     2221 ccagcatgac gacgcccatg tgcgagaccg acgagtacgc gatgacgtac ttgaggtcgc
     2281 gctgcgccat cgcgctcagc gccccgtaca cgatgttgat gcaggcgatc gtgccgacca
     2341 gccaggccca gtcaaccgtc gcctgaggca gcaggcccat gcccacgcgg acgacgccgt
     2401 aggcaccgag cttcatgagc acgcccgcgt gcagcatcga caccgccgtc ggcgccgacg
     2461 cgtggccgtc cggcgaccag gtgtgcagcg gccagatgcc ggcgaggatc ccgaacccga
     2521 tgtagaaggc gaggaagacc cacgtctgca tcgccgggtc gaactgcatc gtctgcaggc
     2581 tcaggagcga gaacgactgc gagccgccgg cgacgaagag cgccaggatc ccaaccagga
     2641 tgaacgcgga cccgaaaagc agatagagcg tcagcttcat cgccgcgtac tctttcgtcc
     2701 ccacgcccgt tcggccgaac gcccagccga agattccctg cggcctgacc tcgcccgacg
     2761 agccccagat gccgatgagc aggtacatcg gcaggacggc gatctcgtag aagaggaaga
     2821 acacgaacag gtcgagcgag acgaacacgc cgtagacgcc ggtgaccagg atcagcagca
     2881 gcgcgtagaa ctcctggctg cgctccttga cggtccacga cgcgaagacg ccggcgaaga
     2941 tgatgatcga ggtgagcagc gccatcagcg cgctcatccc gtcgatcgcg accaggtacg
     3001 agatgccgac cgacggcacg agtgcgatct cttcgcggaa ctggaacccg gccgccgagc
     3061 catcgtaggc ccagtacacc cagatcgacg ccacgagcga cacggtcgtc ccggcgagcg
     3121 agagccatcg gacgagcagc ggacgatcgc gggcgaggaa catgacggcc agcgccgcca
     3181 cgaacggcgc ccacgtcatc acggaaagga cgggaaatgc gttcatacca gcgcccaccg
     3241 cacccagagc agcagcacga gcaggccgac gccgagacca tacacgtagt cctgggcgcg
     3301 cccggtctgg atggtgcgca tctcgtcccc ggtggtgacc gtccacgcgc tgacgacatt
     3361 gagcacgccg tccacgaggt atcgatcgat ccagccgacc agccgcgaga agccgagcgt
     3421 ggccgctccg aacacgccct cgaagagatc gtcgatccag aactttgcga gcgcagcgcg
     3481 gcggatcggc ccggccagcg acgcgagcga tgcggggtcg atggtgccga gctggtaggt
     3541 gagcaacgcc agcgcgatgc cgcccaccgc ggcgccgatg gcaaccggcg tcagccagcc
     3601 ggggctcgag aactctggct cgccgtgcac gaaggtgaaa tagacgccca cggccatgga
     3661 gagcagcgcc aggatccaca gcggcacgag catcgacgcg ggcggatcgt gttcgtgtgc
     3721 cccgctgtgt ccggtctggt cgtgtcgatc atcgtgtccg gctctaggcg gaccgaagaa
     3781 cgcaaggaac acgacgcgga acatgtagaa cgccgtcagg aacgcgacga agatcagcgc
     3841 gacgaacggc acccgcagcc ccccggccca cacggcgccg aggatctctt ccttcgacag
     3901 gaagccgccg aagaacggga tacccgcgag cgagagcgtg ccgatcagaa agacgatggc
     3961 cgtctgcggc atgcggcggg ccaggccgcc catccgcggg aggtcgttgc tgtggacggc
     4021 atgaatgacc gcacccgccc cgaggaagag cagcgccttg aacaaaccgt gcgtgaggag
     4081 atggaagaag ccggcggccg agaagccggc gccgatcgcc gccatcatgt acccgagctg
     4141 cgacacggtg gagtaggcga gcacgcgctt gatgtcgtcc tgcacgcagg cgagcaccgc
     4201 cgcgagcagc gccgtgaacg cgccgatcca cgcgacgacg atcagcacct ccggcgtcag
     4261 ggcgaagagc cacgccgtgc ggtgcagcag gtacacgccc gccgtcacca tcgtcgccgc
     4321 atgaatcagc gccgagacgg gagtcgggcc ctccatggcg tcgggcagcc acacgtgcag
     4381 cgggaactgc gcggacttgc cgacggcgcc gaggtagatg cagaaggtga tcaaccccag
     4441 gccggccacc ggcagccggc cgtcggccac catcaggcgc atctcgccga agtcgaacgt
     4501 gcccgactgc tgccagagga gcacgatccc gatcagcagg cccacgtcgc ccgccttcgt
     4561 cgtccagaac gccttcaacg cggcgcgcgc ggcttcgggc ttcttgtacc agtagccgat
     4621 caggaggtag gagcagagcc cgaccagctc ccagcagatg aagagctgaa cgaagttggg
     4681 cgccagcacc agccccatca tcgagaaggc gaagagcgac tggtaggtgt agtagcgccc
     4741 gagcgcgggc ggcggttcct cgtgcaggta cccgagcgag tagacctgca cgagcagcgc
     4801 cacgagcgcc acgagcgcca gcatcacggt ggagctgccg tccacgagga cgccgatggt
     4861 cgcgagcggc tgcccctgcg ccggcagcca gtcccagacc agccgcgtga cggcctcgcc
     4921 ctggccgcgc cacgccgcca cggcgccggc gagcgatcca agcgcaaaca ggatcgacag
     4981 ataggccgcc ggccgtcccg atctccggag cggcgccacg atggccagga tcaggaacga
     5041 tgccgcggga agaagcagcg acgcgagcga gagataggcc gggtccgcca tcatcccttg
     5101 agcagatcca ggtgatccgc catcaccgtg cggcggatcc ggaagaggag aatcacgatg
     5161 gccagcccca tggccgcttc cgagacggtg atgcagatcg cgaacaccgt gaagatcatg
     5221 ccgacctcgc tgccgctgaa ccgggcgaag gcgacgaggt tgatattgac ggcgttcagc
     5281 atgagctcga tgccgaggag gatcccgatg gtgttgcgcc gggtgacaac gccgaacagg
     5341 ccgatgccga acacgacggc ggcgagcgtg agatacgctt ggagtcccat gtagtcgctc
     5401 attcgcgcag gctgaagcct gcgctctacg ctctctcccc tcgtagagcg ggggcttcag
     5461 cccccgcgtt aatcttctgg ccgcgcaaag tacaccgcgc ccagcatcgc cgccagcatc
     5521 agcagcgcaa gcagctcgaa cggcagcacg aaccgcgtga ggacgctctc gcccagcgtg
     5581 cgcgtcaggt cggctgccat cggcggccgc tccgccggca gcggacgctg cccgatcgcg
     5641 ttcacgacga ccgcgacgaa tgccgccgcc ataacggctc ccgcggcgag gctccgggag
     5701 gtctgcgaga ggcgttctcc aaccagccgc tcggtgacga cgatggcgaa gacgacaatc
     5761 gtcacgatgc cgccggcgta gagcagcaat tggacggccg cgaggaactc ggactcgagc
     5821 agcaggaaca ccccggccgt gcccgtgagc gccagcgcga gccagaggac ggagtggaac
     5881 agattcttcg agagcacgac ggccagcgcc gagccgacga gcacgacggc gaggctccag
     5941 aaggcgagtg tctcggccat cacgcggcgc cccccggtgg tggtgccgtg tccgtcttcg
     6001 cgtgtgcagc ctcggccctg gccatcttcg gggggccctg catttcacgc agccggttgc
     6061 ccgtcgccca ggacacctgg tgctgcaggc cgagcgcgtg cagccggtcc ttgtcgagca
     6121 gcatctcgcg gcggtcggcc gtcggcatgt cgaaggactt cgtcatgacg atcgcgtcgg
     6181 tcgggcacac ctgcacgcac agctcgcaga actcgcaggc gtagagctcg agcgtgaacg
     6241 tcttcgcgta gttgcgcttc tcgctcttga gcatttcgac cttgatgacg gccggcgggc
     6301 agacatactc gcacaggcgg cagccgatgc acgcctcctc gccagtgtcc ttctcgtaga
     6361 cgagcgccag aaggccccga tatcgatccg ggtacgcgcg ggtcacgtcc gggtagtgca
     6421 ccgtgaccgg cttgcggaac aggttgacga gcgtcaccca catcgcgcgg ccgatcgcac
     6481 gcaggaccgc
//
LOCUS       DBNGSH010000060        33846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3060690, whole genome shotgun sequence.
ACCESSION   DBNGSH010000060 DBNGSH010000000
VERSION     DBNGSH010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 33846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33846
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3060690"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="AC16CY_01330"
     CDS             <1..660
                     /locus_tag="AC16CY_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3352444.1"
                     /translation="VDPQGNLTSGIGFKSQRSDGGTVYEALLAGGPPESFVLPTRIPN
                     LSLIPSDRNLTGAEIEMVALPEREHRLKRLLHPLRDRFDHLFVDCPPSLGLLTLNALV
                     AADAVLIPLHCEYFALEGLADLVGTMRRVRGGLNPSLDIEGVLLTMYDDRTNLGQLVA
                     RDVREFFKEKVFNTIIPRNVKLGEAPSHGMPGVLYDAKSRGAAAYAALAREVLARYAA
                     A"
     gene            675..1544
                     /locus_tag="AC16CY_01335"
     CDS             675..1544
                     /locus_tag="AC16CY_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3352445.1"
                     /translation="MLERRPALGKGLSALIPDVPEPIRTGSLEVDIDLLSPNEQQPRL
                     HMDDARLEELTQSIKSNGIIQPILVRRDGQGYRIIAGERRWRAAQRAGLHKVPVVVRE
                     VTQGDKQALELALVENLQRENLNPVDEALAYQRLANEFAMTQEQIAAAVGKDRSSVAN
                     FMRLLKLPDEVRGDLSAGALSTGHARALLALPDAATQRQTAREVVSRALSVRETEALV
                     KKLLAQSTSRQEPAAAARTKDVHTRAAEDRLRFALGTKVQIVRRRQGGRIEIEFTSEN
                     ELNRLFERFTAQR"
     gene            1577..2059
                     /gene="rfaE2"
                     /locus_tag="AC16CY_01340"
     CDS             1577..2059
                     /gene="rfaE2"
                     /locus_tag="AC16CY_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HIR3352446.1"
                     /translation="MRVPSRRSLSFEEARAFSKDVRKRGGIVVFTNGAFDVLHPGHIH
                     SLKEARGLGDALVVGVNSDRSVRANKGPDRPINRAEARATVLLGLLFVDAVVVFDEDT
                     PLDLIARIEPDVLVKGADWSADNIVGRDLVEARGGRVVRLDLTPGFSSTSLIQRTRGK
                     "
     gene            2072..5599
                     /gene="mfd"
                     /locus_tag="AC16CY_01345"
     CDS             2072..5599
                     /gene="mfd"
                     /locus_tag="AC16CY_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3352447.1"
                     /translation="MATTTPSLTLRSILGRAVSQARLDQGAPVLAGLTASAKALAAAG
                     LARSRGGVTLVVVPTEADVERFIADLRFFYAGLEAASDAALDHAILPLPAIQVDPYRG
                     MTPHFRVAAGRARALHAAAAGTARVIVASSAALLPRVSSPARLLATSVELRVGSECEP
                     QRLADLLIEGGFTREDPVDEHGAFALRGGIVDVFPTADAEPVRIEFDGDMVETLRRFD
                     PSTQRSTGAIDLVQIVPARERFDDDEPTSVMEFLASSPAGLNLIVSEYEEVRQQALKL
                     REQLQRSYEETSARGRPAAVPPALAFTDWEDLAARTAKALRLEELTIADDAAARHVSC
                     QPAIEFRGRIGDWAADIKRARESGATVLVVADSPGKAERSAEILRDYDIIAVPLDRAE
                     EAHAGAVLIAIGALSKGFRLEDADLQIYSEADVFEEERRITEGRRSLSKTFLSDLRDL
                     KVGDLVVHVDHGIGEFVGLKQLATGSRSTPATGADLQEFLELRYHGDDKLFVPVERLD
                     LIQKYTGGSRPALDRLGGTTWEKAKTRVKKAMRDMAEELLKLYAQRKAMPGHAFAADT
                     HWQEEFEGAFPYEPTPDQATAIADVKRDMETATPMDRLLCGDVGYGKTEVAMRAAFKA
                     VMDGKQVAFLAPTTVLAFQHLKTLRDRFAAFPVTIDMVSRFRTRQEIKEVVGRVAEGK
                     VDIIVGTHRLLSKDVQFKDLGLLVVDEEQRFGVVHKERIKQMRKRVDVLTMTATPIPR
                     TLNMSLVGIRDMSIIETPPKDRLSIQTNVVKFDAAVIERAIRNEIARGGQVYFVHNRV
                     ESIFSIGNLVQRLVPEARIAIGHGQMGEEELERAMLTFVDRRCDILLATTIVENGLDI
                     PNANTIIINRADRYGLAQLYQLRGRVGRSDRAAYAYLLIPPPESLSPVARKRLAAIRE
                     FSDLGSGFRVAALDLEIRGAGNLLGGEQSGHIDAVGFEMYVKLLEEAIRQLRGEELEE
                     EARATVNLRIDLRIDDSYIPDMNQRLMVYRKVASARTEPEVNSLLEELRDRYGAPPES
                     VLNLAEYGRTRIMADRLEVDSIDREGRMIVIRFRPNARLDGLHLVKVVSGWPGATLVP
                     PVSVTLDLEAAPEPSAEARRAGPKRGDTSRGRSHAAQGSWWTARALAGEVKAGFNKAE
                     VLRVPERSPRAEDGLFSRLLSLLQALDAG"
     gene            5653..6726
                     /locus_tag="AC16CY_01350"
     CDS             5653..6726
                     /locus_tag="AC16CY_01350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3352448.1"
                     /translation="MFKRTVVAMAMAACAASATVGAEILEQVLVRVNGEIITKTEFEA
                     RQVAALGNRPELAGAGPSSPELRKAVAELTPDLILSAVDDLLLVQRGRESGYALGDQQ
                     FTQILENIKTTNDLQDDAKFREALRQEGLTLADLRRNLERQMLISQVQRVEILDKISL
                     TDEEARAYYESHLKEFTAPAEVTLREILFEVANTSRGFNVAEDDAIRARAAEVRKRLI
                     GGEPFPRLAGEYSASGSKANGGLVGPLNVEELSSNLRDLLAGMQVGDLTEPLRTARGY
                     QLIKLESRTDARVRSFEAARGDIGNKLGEQKLRVEREKYLDRLREQATIVWRNAELKK
                     AYETALSRRQAANTQAAAAAPAN"
     gene            6745..7287
                     /locus_tag="AC16CY_01355"
     CDS             6745..7287
                     /locus_tag="AC16CY_01355"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HIR3352449.1"
                     /translation="MSQKSTQPVTEIGPWRDDGENWFALWIRSRHEQVVREQLEQKRI
                     ETFLPTVTRWSRWKDRKKRIDWPLFPGYCFARFDPRDRLPVLKCTGVVNIVSFEGEPA
                     PIPQLEIEGIRRLVQSDLAYDPCPMIREGMMVEVVHGPLTGVVGRLVRKNDKARLVLS
                     VDLIGQAVSVEVDAADVRPY"
     gene            7294..8340
                     /gene="dusB"
                     /locus_tag="AC16CY_01360"
     CDS             7294..8340
                     /gene="dusB"
                     /locus_tag="AC16CY_01360"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR3352450.1"
                     /translation="MKIGSIEFPSPLVVAPMAGMTDTAFRRLVKRQGGCGLVVTEMVS
                     SEGLVRGIDRTLEYAEYTEEERPVALQIFGGNPHTMAEAARIVEGMGADVVDINMGCP
                     VKKIAKHNAGCSLMREPEHAARIVSAMTTAVSIPVTVKMRAGWSDDERNAPTLARMVQ
                     DAGAAAVTIHGRTAEQSYSGLADWDLVSRVAADLKIPVLGSGDCLEPEQIVSRLQAGV
                     AGVLVGRGVLRNPWILAQSSDIRAGRDPRAVTMEDRGRFLLDYIDLLIDQRENEARGF
                     RHTAPGPLPDAAPASGRERWVINKIRALCAWYSKGLDAGSHLRVRVNSVDRVAQLRRA
                     IEEYFLANTSVAPA"
     gene            8354..8932
                     /locus_tag="AC16CY_01365"
     CDS             8354..8932
                     /locus_tag="AC16CY_01365"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR3352451.1"
                     /translation="MDSDVSRQLRQIAEGLRAISEGSYAAQVERALDILETAFRAGGT
                     LLVFGNGGSAADAEHLCAELVGRFSADRRPLPAIALTTNQAFLTAWSNDRQFDDVFAR
                     QIEGVGRPGDVAWGISTSGNSPNVVNGLRRARELGLRTLGLSGATGGPMAEWCDALMA
                     VPLDQTPRIQEAHLVTYHTICGELERRLFTRG"
     gene            complement(8946..9947)
                     /locus_tag="AC16CY_01370"
     CDS             complement(8946..9947)
                     /locus_tag="AC16CY_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR3352452.1"
                     /translation="MMFLRAGSNAAARIDDRIQPVSDEIVDASVVDEVANIFSRGGDG
                     RWQPRPDGEWVREDEEFGYVSCRVFSDLDGTGLVVQLRPTTSLRLLHKHIPRQIRLAC
                     EAQGLVVVAAGNETDVEMLAAAVADWSSRDRGGYLISLQRRSRSLEDIAGVFVSRRTI
                     SGSEKDFAAAIRRASQEAPDILLVTGLQSEQALQSAVLAAAGGRLVIVGVVASTTVDA
                     LRMLSGSDVHVRRAMAASFRAAIGYRSLRRIGGGRTLVQDVVLATSAVCGALEAGDFD
                     RLARMQQDGGPGMRSVDESLARAVARRHVALREAVAQASDRGQMVSLVRSLARRRAH"
     gene            complement(10544..11020)
                     /locus_tag="AC16CY_01375"
     CDS             complement(10544..11020)
                     /locus_tag="AC16CY_01375"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3352453.1"
                     /translation="MSDPGSDSDDEMVVKLPEKASLDDLFAGLEASWQSPAAAKAQSP
                     AEAKPDPVPEPPPQRKERRGAPRREPHQFKVEIRLAVAGFPMRLVNLSSTGLLAETSQ
                     RLCPGRTVDVFLRYGGVRKVLRATVIRSSMHAVAPTPVFRAALQFEDTLTLQDADL"
     gene            11209..11574
                     /locus_tag="AC16CY_01380"
     CDS             11209..11574
                     /locus_tag="AC16CY_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3352454.1"
                     /translation="MAKILVVEDDDATRAGYRELLSRAGHEVVATATYQEGRHAVATE
                     SPDLVIADLRLGGFNGLQLLLLSPQPIPTIIVTGFHDQVLEAEARRAGADYVVKPVSP
                     SNLMTLVRERLESQAASGR"
     gene            11796..13502
                     /locus_tag="AC16CY_01385"
     CDS             11796..13502
                     /locus_tag="AC16CY_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352455.1"
                     /translation="MSSIKSQLPVAQEPTLFEGAVRDRSWEWLLELRRRLKVELLLVD
                     GRCSVLLPARDQAARLQGLLEQTEPVLVSAINATLRSRTAHVVELGGLQAVCLAISRD
                     RGDAGALLVGRTVPPGQDPRASRDQLQLVTSWLATAVEAHLLSPPSLLSSGLSRVAPL
                     AQVFGQCAEHESDRELVRLFGEAVAVWHDIEVCGYVETSNGGFARDVTLPGANKGERP
                     LSISAEELPEATELTRLPHGHLDRFGLPVSCDVYVSRLARAAERSWLLVFIGAIDDYD
                     LQRLGAYLALLELALALSTSTMTTRVVNTIGSRLSDVQQTPEMRTRNALEELRRTLGA
                     SSAGLKIESPDGAPLFDALSPDLADTSRADPGASRLVLVKRSEQHYTTTVSLGRYESR
                     QFTPRDYAVASAAATVFGLWVPATLSTAAGRRERRALPRGFQEVIERSACEAIERGVP
                     VAVVVLLIRDAISLPGSTQRWVAGIRGQMRASDVAGMLTEGEIGLLLHDIANEQAKAI
                     AERLRTVVSGAPGREAILIGVASWTPGQDPADGIVRRARADALADGRRAQVSVSPRGV
                     NS"
     gene            13502..13819
                     /locus_tag="AC16CY_01390"
     CDS             13502..13819
                     /locus_tag="AC16CY_01390"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3352456.1"
                     /translation="MALGDRRSRLRLEVVGALWATLELTEPARVVNISRDGALITSPV
                     PMTPESVQPLHLKLEGREVTVDARVRHIRRVSGPDEPIRYLVGVEFLTVPPALARATE
                     WPS"
     gene            14065..14376
                     /locus_tag="AC16CY_01395"
     CDS             14065..14376
                     /locus_tag="AC16CY_01395"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3352457.1"
                     /translation="MEERRRTPRQQVGEEFANLPGNMSVRVLDISTVGVLIQSPRLLN
                     PGSRGSLRFTLAGAPFAADIQVRRVSSASDNGSGYRIGATFVGMTMDHRQLIERFMNN
                     D"
     gene            14369..15496
                     /locus_tag="AC16CY_01400"
     CDS             14369..15496
                     /locus_tag="AC16CY_01400"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3352458.1"
                     /translation="MIDGVAESSTRVGAIGPIGAIRPRSHVQLTGRSAEVVALEQEID
                     RVARSDAKVLITGESGVGKEIVARAIHSRGPRSGHVFAPVNCAGLPETLLESELFGHV
                     KGSFTGAYRDKPGKLETAHMGTIFLDEVGEMTLRMQGLLLRFLETGELQKVGADGSGR
                     IVNVRVIAATNRDLRDMIKQGTFREDLFYRLNVIHIVVPPLRDRREDIPVLANQFLSH
                     FTTAGRSTITGISSDAMKALIEYSWPGNVRELENVIERLVVTVSGPTIETEHLSSEIR
                     AYDAVMLRPKRERRRTVADDLYKRMLEQHESFWTAVYPLFMDREITRANLREVVRRGL
                     EEARGNYKIVARLFNMEARDYKRFLNFLRKHDCQIPFKEYR"
     gene            15808..17223
                     /locus_tag="AC16CY_01405"
     CDS             15808..17223
                     /locus_tag="AC16CY_01405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3352459.1"
                     /translation="MLLELHLKIDRTSPPREFLRGLVNAISEALQTTVAVVGRNGDVW
                     VRAAASGPATPRLPLQSEGWPQIFDRLSATAGAQLELWRTGSEDWTLFGLARAPHTPV
                     ILILEGDWTHWAAVLQQMAQGLSIGDVRDLSQARPLDSILQRLSHALADTVGLAEVGE
                     VVLRHVIQAVPSRLASLASAAPDGELSIVATQGYPRELVQHLRIASGNGVIGAVHLSR
                     TPLVVSDVSLTPGLDRHRSRYRSNSFVAVPIVAGSETLGVVSLTDRVASGPYTQGDVA
                     AITVLMGPAALALARERVRQEALAYAQAAVVDPVSGLFNRRYFEARLDEELHRAVRQL
                     TSVALLMVDLDEFKAVNDRFGHIAGDLVIRDISEILRRSVRIFDVCTRFGGEEFAVMM
                     PGGTVESASAIAERIRHRVEAYQRNEPSLAGLHVTASIGVAVSPPGVTARDLMERADR
                     ALYHAKKAGKNRVSTAGTDPV"
     gene            17616..18785
                     /locus_tag="AC16CY_01410"
     CDS             17616..18785
                     /locus_tag="AC16CY_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3352460.1"
                     /translation="MPKNLPLLLGESPAISSLRREIEAAARTDAKVLVLGETGSGKEV
                     VARLIHEQSGRSSRAFVAVNCSGIPETLLESELFGHTRGSFTGAYRDKPGLVRQADRG
                     TLFLDELGEMSLRMQAVLLRFAETGEIQTIGVDGVTGRSDVRLITATNRNLRERIDAG
                     VFREDLYFRLNIIQIHVPPLRERTEDVMLLLRHYLRRASETHRLAYPELTLDAEQVLA
                     SYAWPGNVRELKNLAERLVVREWDRAIGANDLPAEIRGGRDTVSTSPSSSVTFNQDSV
                     PTARRLERGSGTADRLWERLIAGESFWTVVHQPFKAHELTRDDLAALIHRGLEHTRGS
                     YRALLGVFGLPPTDYKRFHAFLYQQNCNLPVGPYRTARATLRRQDRPASVYTPAC"
     gene            complement(18848..19201)
                     /locus_tag="AC16CY_01415"
     CDS             complement(18848..19201)
                     /locus_tag="AC16CY_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR3352461.1"
                     /translation="MIVHAVLFRPKARLSTADRRALVDAFSTAIKEIPSIKRARIGRR
                     TLLGRSYEQLMKDDYTHAAFLEFDDVAGLRAYLDHSAHQQLGARFLACFEQALIYDFD
                     TTGEDQALNALLRED"
     gene            complement(19644..21023)
                     /locus_tag="AC16CY_01420"
     CDS             complement(19644..21023)
                     /locus_tag="AC16CY_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3352462.1"
                     /translation="MAAAGTIPHILFVDDETALRGVMAERLTERGFEVVQAESGERAL
                     ELLEKFAFDVVITDLRLPGVNGSRVIESARERYPGIVAIVITGFGTVKDAVDAIKRGA
                     SDFIAKPFQFDELSHVLHKALEDRRLRSENAYLRSQLEDRYQFGGILGRSRPMQKLFH
                     LLETVARSSSTILITGETGTGKEVVARAIHHNSPRRAHRFVALNCSAIPETLLEAELF
                     GHVRGAFTGAIGARQGRFEQAHKGTLFLDEVGTMSTALQMKLLRALQEREFERVGDNQ
                     TIKVDVRVIAATNSDLARMVAEGTFREDLYYRLHVIPIHLPPLRDRRDDIPVLAQHFL
                     QKFAPGSQMQVSQDAMRALMAYQWPGNVRQLENAVERAVALSAGRQHIEMADLPAEIQ
                     VTPPATAMPLVDFPEGGMDMPAYLAAVERDLIGRALDRTGGNRNRAAELLRIKRTTLV
                     EKLKRIGPD"
     gene            21160..21375
                     /locus_tag="AC16CY_01425"
     CDS             21160..21375
                     /locus_tag="AC16CY_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HIR3352463.1"
                     /translation="MDRQTLSIMKACEAVGVSRRTIYNWISAGKVEYVRTAGGSIRIF
                     ADTLWRDAATPARTQESDSRTAPGQHA"
     gene            21372..21611
                     /locus_tag="AC16CY_01430"
     CDS             21372..21611
                     /locus_tag="AC16CY_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352464.1"
                     /translation="MSVPQGVDGVGDVPANLDLQALFHRLNNQLGSLLAHAELLETKA
                     PDDVNRARAAQVVRSTLDAMGTVRAIYHQTDPSGE"
     gene            21908..22588
                     /locus_tag="AC16CY_01435"
                     /pseudo
     CDS             21908..22588
                     /locus_tag="AC16CY_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(22744..24294)
                     /locus_tag="AC16CY_01440"
     CDS             complement(22744..24294)
                     /locus_tag="AC16CY_01440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3352465.1"
                     /translation="MAAVPRRVPDGQRANAFARLFEAVHEGVYIGAIDSADDRPGETL
                     AANPHLKSILGFPADTPDQNVLPFEPESFVEATARTAFLDRLLNDGAVTDYLLRLRRH
                     DGDSIWVEVTARTDRDRSGTRVRVEALVRDVSERKRLDEQSRDLYQQLLQAEKMAALG
                     QTISGVAHELNNPLATILSWAERLAEKASDETTRRGLDVILGESERAARIVRNLLTFA
                     RKRQSTRSLLDVNQVVRETLALRSYEHRRHIEVITALASGLPKVLADAHQLQQVLLNL
                     VINAEQAMMAAHGRGSLVLRTWHDLERDAVVLQVTDDGPGIPVDVKSKIFDPFFTTKE
                     TGKGTGLGLTVAYAIVQEHGGSIRVDSPATGGASFTVELPAGSVDPTTRAPGAAASRS
                     ELTQRASVLLVEDERALAMAVSEALIDAGLRVDHAGDGEEALARVHQRSYDLVICDLK
                     MPKVDGITLYRAIAAATPALARRVIFVTGDVAGADAERFLEESGCRWLAKPFRLGDLL
                     RNVREALA"
     gene            complement(24356..24883)
                     /locus_tag="AC16CY_01445"
     CDS             complement(24356..24883)
                     /locus_tag="AC16CY_01445"
                     /inference="COORDINATES: protein motif:HMM:NF025170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HIR3352466.1"
                     /translation="MPFRPTATRGLTLTLCTVLLVSAGIGLVQHTYAQTSRAPSPPAT
                     VKAHSGPLPAIPSVPFTPPRPMATVRQVYEFAARHPEVLKYVPCYCGCERVGHNGNHD
                     CFVKSRAANGTVTAWDSHGMGCAICIDVGLDALALFNTGASPSQIRAAIDRKYGSSFP
                     SSTPTPRPAHDAKQH"
     gene            24973..26163
                     /locus_tag="AC16CY_01450"
     CDS             24973..26163
                     /locus_tag="AC16CY_01450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3352467.1"
                     /translation="MRLAGRNDVVLMVGLTVALFVIFSQPVSRLLDYIREIEAARGLH
                     LLPALFILATVFSFHQIRKRQEMREEARAVAADANSATERASEMSRLVAFGHALARAM
                     DSDSSDSIRDVAVAHIPLLVPGRNTWVMTRVRSGHWESLVAIGESSPIERERAARRAL
                     GEAEPLVGLAPTEICFPMIVAGTPEGVLGVAPEPPLTNHQRSVLSAAAALLAVSLKNS
                     QLLREVRENSVRDGLTSCFNRGHALEVLDAEIRRARRSKLPLSLVMFDLDGFKEVNDQ
                     YGHLCGDAVLASVGTKMKSELRGSDLKCRWGGDEFMVILPDTPLGGARQVSENLRRQI
                     ETQTVAWNDDYVKVTASFGITVINPADQDPLTAVARADAALYRAKERGRNNVQVQEQP
                     AALV"
     gene            complement(26160..26633)
                     /gene="fabZ"
                     /locus_tag="AC16CY_01455"
     CDS             complement(26160..26633)
                     /gene="fabZ"
                     /locus_tag="AC16CY_01455"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR3352468.1"
                     /translation="MDPLTLPLDYSAIERILPHRYPFLLVDRITELEPDKRIVGVKNV
                     SLNERCLAHHPGEAASLPPTILTECIAQVGAILILTKPENRGRIPLFRGIERVRFRRQ
                     VYAGDVVEIEARVKSLRSRVGQLHGYARVNGRVVVDGTMTFALGPAPRTSGEGAP"
     gene            26695..28500
                     /locus_tag="AC16CY_01460"
     CDS             26695..28500
                     /locus_tag="AC16CY_01460"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3352469.1"
                     /translation="MSDQVPHPPQPVARDQGSGGRVPTDTELLSTLFDLGREVTSVLD
                     LEELLAKIPQIIARLTRFSAFSVYLLDEKRQELRIAYAVGYPEEASNLRLPVGQGVVG
                     AAVAEGRPILVNDIRQEPRYRGPLRNMLSQLAVPLRRKGRVIGALNLLNEAEGAFSTQ
                     DEALLRQFGAHVAVAIENARLFKSERQYNDTLETLAEIGRDLSSILDLDALLTRIASL
                     MKRLIDYRTFGILLLNEDSGELEMKFAVRYGQGAESKHIKLGEGLVGWAALHKEPVLV
                     ADVSKDPRYINVVADVRSELVLPMLVKDRCIGVFDLESPELDAFSKEHQELLTLLAGQ
                     AAIAIDNARLYEEVRHNEERIERELRFARRVQHALLPVGPPKTLREADVAARYESAHE
                     LGGDIHDFLAPKRDALVVAVGDVSGKGAPAALYGMFAAELVRSHTMVRRFSHERFSVS
                     GVLKAINTLLYERQLEAYYCTLCYAFFDFSRRVLTLSNSGLPYPIQCTATSCGQIELP
                     GLPLGSFPGATYDEVAIDLHKGDIFVFATDGILETLNDGGVEFGALRLAEVVQSNRDQ
                     SARTIVDRIFDAANGFRGSRPQADDMTAVAVKITV"
     gene            28842..29681
                     /locus_tag="AC16CY_01465"
     CDS             28842..29681
                     /locus_tag="AC16CY_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3352470.1"
                     /translation="MRKMIVAGGILAAVALVAGVVVALAPSAVAQQGRYSPFLITEGP
                     GSSIGVAVRDSDTDEARKAGTNGVVVQEVREGTPAARAGVRTGDLIVDFDGERARSAS
                     QLTRVVRETAPGRAVKMTIVRDASRQTLDITPEARGADNIMSNIVANVRTTPGVDGRN
                     FAFDYLTFDGAFSRQRLGVTVTQLTDQLATYFGVKQGVLVSEVTSGSPAETAGVKAGD
                     IITTVNSRAVSTADDVVREVRAAAPGSAVEVRVMRDRKQLTLTAKVPEPSRPPTVFSG
                     RTI"
     gene            complement(29785..30873)
                     /locus_tag="AC16CY_01470"
     CDS             complement(29785..30873)
                     /locus_tag="AC16CY_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352471.1"
                     /translation="MRHVDEVERISQDNLRRIDRLEADLDEIAQEELTYAASGGAAGP
                     ALERTSNAMHHLASETPPLLARLLSENSTPARAAAAASSTIAEIEGSARENVRAGLDL
                     MAADLIFSETHMLRGELRQQFRALRAEEIAAAAVARASDLRTIAAAMGLTALLFAWAL
                     VRATRGPSSSSTPVHESVTPSAPAAADRSVFELDLKPAPLDLPAVANLCTAIGRMKSR
                     EDLERLLERTGNLLAAPGVVVWMAGGEELFPAAAYGYDLGQLGRLGPIAKGAVNATAA
                     AWRTGQLQTVGGEATSRGAVVAPMLGTDRCIGVLAVEVNSERVSDKTTQATVSLVAAQ
                     LSAVVAPWPAGSSTEPADILPFERTASS"
     gene            complement(31004..32734)
                     /locus_tag="AC16CY_01475"
     CDS             complement(31004..32734)
                     /locus_tag="AC16CY_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014713.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HIR3352472.1"
                     /translation="MRGLVSVVALLLVALQVAAAQLRGIQAELAPIVESDAAAGASVR
                     AALQVRLPDRFHVQSDAPRDPSLIPTVLTIDPPDGVTVGEIVFPEATDFEQIGQAEPL
                     AVFEQEFAIGVRLDVAPGAPLGAVSVPATLRYQACDERLCYPPITADLHWPLTIVGAG
                     APRTARHPEVFGRIAFGRGRAPVARESIPIAPRGTTADSTELLDQFTMVATTGGYQGT
                     DAFLQFVRDAESGVAPRGLFEGRGPVAILLLVLLGGLALNLTPCVLPMIPINLAIIGA
                     GAGSGSRGRGFLLGAVYGGAMAAVYGVLGLIVILTAGQFGTINSSPWFNLAIAVVFIA
                     LALAMFDVLTIDFSRFSGTLGTGSQRGSLALAFGMGAVAALLAGACVAPVVIQVVLFS
                     SDLYARGTAAALALPFLLGIGMAVPWPLAGAGLTALPKPGTWMVRVKQGFGVLILATA
                     AYYGYLAYGLFADRWVDAREVASSIEEKLKAGWYGSLREGLAVAQRDQKPVLIDLWAT
                     WCKNCLVMDRTTLADEKVKAALDGYVKIKFQAESFNEEPVRSVMNRFNAVGLPTYVVL
                     HPKQLPATPR"
     gene            complement(32737..>33846)
                     /locus_tag="AC16CY_01480"
     CDS             complement(32737..>33846)
                     /locus_tag="AC16CY_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3352473.1"
                     /translation="TRCSKGCNTSAWLKAKPEWAKGSRLIRMTPRLNLDVNGYWMCDI
                     GRFEYSWVEGETRLRRPMRRRGSTLEPAAWHDVEPGLRDAIQIAGQADPGSVRFLVSA
                     HAATEELFVLKEIVDGLLGEAGAGIVTVAWTRSEKRQPASARFTIPVTDAPNVNGARD
                     VGFAVGGGNGHDADLGALRTAVGSGRVKALYVLDPGPDGSLGDVSWIVEARRKGTLPM
                     LVVQGVVMSDLAAAADIVLPGAASAEKDALYTNDQGKVQGASRAINPPGEAREDWQIL
                     VTLGRLLGSSLPHTTIDEVRRAIAAKLPAAYADAERIAFTRPVPARNWLQASNPSERW
                     KWDFLYQDAPPVKGHNVQMEAESPASPYIPLKPVG"
BASE COUNT         6219 a        10797 c        10923 g         5907 t
ORIGIN      
        1 gttgatccac agggaaatct gaccagcggc atcggcttca agtctcaacg atccgatgga
       61 ggcactgtct acgaggcgct tctcgccggc ggtcccccgg agtcgtttgt gcttcccacg
      121 cgtatcccga acctctcgct cataccctcc gaccgaaacc tcaccggagc ggagatcgag
      181 atggtggccc ttccggagcg ggaacaccgg ctcaagcgcc ttttgcaccc cttgcgagat
      241 cgcttcgatc atctcttcgt cgactgcccg ccgtcgctag gcctgctgac gctgaacgcg
      301 ctcgtcgccg cagacgcggt cctgattcca cttcattgcg agtacttcgc cctggaagga
      361 ctcgcagacc tggtcggaac gatgaggcgt gttcgcggtg gattgaaccc ctcgctcgac
      421 atcgagggcg ttctcctgac catgtacgac gacagaacca acctcggtca actggtagcg
      481 cgtgacgtgc gtgagttttt caaggaaaag gttttcaaca cgatcatccc tcgcaacgtg
      541 aaactgggcg aggcgcctag ccatggcatg cccggcgtcc tctacgatgc caagtcacga
      601 ggcgccgctg cgtacgcggc gcttgcccgg gaagtgctcg cccgctacgc cgccgcctga
      661 aggagaacac agtcatgctc gaacgccgcc ccgctctggg aaagggtctc agcgcgctga
      721 tcccggatgt gccggagccg atccgaacag gttcgctcga agtcgacatc gatcttctgt
      781 cccccaacga gcagcagccg cggctccaca tggacgacgc caggctggaa gaactgacgc
      841 agtcgatcaa gtccaacggc atcattcagc cgatcctcgt acgacgcgac gggcaggggt
      901 atcggattat cgccggcgag aggcgctggc gcgccgcgca gcgagcggga ctgcataaag
      961 tgcccgttgt cgtgcgcgag gtgacccagg gcgacaagca ggcgctcgag ctcgcccttg
     1021 tcgagaatct ccagcgcgag aacctgaatc ccgtggatga agcgctcgcg tatcagcgcc
     1081 tggcaaacga gttcgccatg acccaggagc aaatcgcggc tgcagtcggg aaggatcgca
     1141 gttccgtcgc caatttcatg cgactgctca aactcccgga tgaagtacgt ggagacttgt
     1201 cggccggcgc tctttccacg ggccacgcgc gcgccctgct ggcgcttccc gatgcggcga
     1261 cacagaggca gaccgccaga gaggttgttt cgcgggcact gtcggtccgc gagacggagg
     1321 cactcgtcaa gaagctcctc gctcaaagca catcgagaca ggaacctgca gcagccgcac
     1381 gcacgaaaga cgtgcacact cgggctgccg aagatcggct gcggtttgcg ctcggaacaa
     1441 aggtccagat cgttcgtcgg cgacaaggtg gcaggatcga gatcgagttc acgtcggaga
     1501 acgaattgaa ccgcctgttc gaacggttca ccgcgcagcg ctgacctggc tgagagtcgc
     1561 gacggattgc gagtctgtga gagtcccgag ccggagatcg ctgtcgttcg aggaggcgcg
     1621 cgcgttttcg aaggacgtgc ggaagcgtgg cgggatcgtg gtgttcacca acggagcgtt
     1681 cgacgtgttg caccccgggc acattcacag cctgaaggag gcccgagggc tgggagacgc
     1741 gctcgtcgtt ggcgtgaatt ccgaccgttc cgtccgcgcg aacaaggggc ccgaccgacc
     1801 gatcaaccgc gccgaggccc gagccacggt gctgctggga ctgctctttg tggacgcggt
     1861 ggtcgtgttc gacgaagaca ctccgctcga tctcatcgct cggatcgagc ccgacgttct
     1921 cgtcaagggt gccgactgga gcgctgacaa catcgtcgga cgcgacctcg tggaagcacg
     1981 gggcggccgc gtggttcgcc tcgatctgac tccaggtttt tcaagcacga gcctgatcca
     2041 gaggaccaga gggaagtagg tccggggcca catggcgacc acgacaccct cgctgacact
     2101 gcgctcgatc ctgggccgtg ccgtctcgca ggccaggctc gaccagggcg cgccggttct
     2161 cgccggcctg acagcctcgg caaaggctct ggccgcggcg gggctggcgc ggagccgcgg
     2221 aggagtgacg ctggtcgtcg tcccaacgga ggccgacgtc gagcgattca tcgccgacct
     2281 ccgcttcttc tacgccggcc tcgaggccgc ttccgatgcg gcccttgacc acgccatcct
     2341 gcccctgccc gccatccagg tcgatccgta tcggggaatg acgccacact tccgcgtcgc
     2401 ggcggggcgg gcgcgcgctc tgcacgcggc ggcagccggc acggcacggg tgatcgtcgc
     2461 gtcctcggcc gcgttgttgc cacgcgtcag ttctcccgcc cgtctgctgg ccacgtcagt
     2521 cgaactccgc gtaggcagcg agtgtgagcc tcagcgcctg gcggatctgc tgatcgaggg
     2581 cggattcacg agagaggatc ccgtcgacga acatggcgca ttcgcccttc gcggcggcat
     2641 cgtggatgtc tttccgacag ccgacgccga accggtgcgc atcgagttcg acggcgacat
     2701 ggtcgagacg ctgcggcgat tcgatccgtc gacgcagcga tcgactggtg cgatcgatct
     2761 ggtccagatc gttccggcgc gggagcggtt cgacgacgac gagccgacgt ctgtgatgga
     2821 attcctcgct tcctcgccgg cgggcctgaa cctgatcgtc tcggagtacg aagaggttcg
     2881 gcaacaggcg ctgaagcttc gcgaacaact gcagcgcagc tacgaggaga cgtccgcacg
     2941 aggccgcccg gcggcggttc ctccggcgct tgccttcacc gattgggagg atctcgcggc
     3001 acgaacggca aaggctctcc gcctcgaaga actgaccatc gcagacgatg ccgcagcgcg
     3061 ccacgtgtcg tgtcagccgg cgatcgagtt tcgaggtcgt atcggagact gggccgcgga
     3121 catcaagcgc gccagggaga gcggtgcaac ggttctcgtc gtagctgaca gccccggaaa
     3181 ggccgaacgc agcgccgaga ttctccgcga ctacgacatc atcgccgtgc cactcgaccg
     3241 ggcggaggag gcacacgccg gggccgttct gatcgccatc ggagcgctgt cgaaggggtt
     3301 tcgactggag gacgccgacc tccagatcta ctcggaagca gatgtcttcg aggaagagag
     3361 gaggatcacc gaggggcggc gaagcctctc gaaaacgttt ctttcagacc tgcgcgacct
     3421 gaaggtgggc gatctcgtcg ttcacgtcga tcatggcatc ggagaattcg tcgggctcaa
     3481 gcagctcgcg acgggcagtc gttcgacgcc ggcaacggga gcagacctcc aggaattcct
     3541 cgagctccga tatcacgggg acgacaagct gtttgttccc gtcgagcgcc ttgacctcat
     3601 ccagaagtac accggtggct cccggcccgc tctggatcga ctgggcggaa ccacctggga
     3661 aaaggccaag acgcgagtca agaaggccat gcgggacatg gccgaagagc tcctcaagct
     3721 gtacgcgcaa cgcaaggcca tgccggggca cgccttcgcc gccgacacgc attggcagga
     3781 ggaattcgaa ggcgcatttc cgtacgaacc cacgcccgac caggcgaccg ccatcgcgga
     3841 cgtcaagcgc gacatggaaa cggcaacgcc catggatcgt ctgttgtgcg gcgatgtcgg
     3901 ctacggcaag accgaagtgg ccatgcgtgc cgccttcaaa gccgtgatgg atggaaagca
     3961 ggtcgccttc ctcgcaccga cgacggtcct ggcgtttcag cacctgaaaa cgctgcgcga
     4021 tcgatttgcg gcgtttcccg tcaccatcga catggtcagc cggtttcgga cccggcagga
     4081 aatcaaagag gtcgtcgggc gtgtcgccga aggcaaggtc gacatcattg tcgggacgca
     4141 ccggttgttg tccaaggacg tgcaattcaa ggacctcggc cttctcgttg tcgacgagga
     4201 gcagcgcttt ggcgtcgtcc acaaggaacg catcaagcag atgcgcaagc gcgtcgacgt
     4261 gttgacgatg acggccacgc cgattccgag gacgttgaac atgtcgctcg tcggcatccg
     4321 cgacatgtcg atcatcgaga cgccgccgaa ggaccggctg tcgatccaga cgaacgtcgt
     4381 gaagttcgac gccgccgtta tcgagcgggc gattcgcaac gagatcgcgc ggggaggcca
     4441 ggtctacttc gtccacaacc gcgtcgagtc gattttctcg attggcaacc tggtgcagcg
     4501 gctcgtgccg gaagcgcgaa tcgcgatcgg tcacgggcag atgggggaag aagaactgga
     4561 acgggccatg ctgacgttcg tcgatcgacg ctgcgacatc ctgctggcga cgacgatcgt
     4621 cgaaaacggg ctcgacattc cgaacgcgaa caccatcatc atcaatcgcg cggaccggta
     4681 cgggctcgcg cagctgtacc aacttcgagg gcgagtcggc cgatcggatc gcgccgcgta
     4741 tgcatacctc ttgatccccc cgccggagtc gctgtctccc gtcgcgcgca agcggctcgc
     4801 ggcaatcagg gagttcagcg acctcgggag cgggttccga gtcgcagcgc tcgacctgga
     4861 gattcgcggc gccggcaatc ttctcggggg ggagcagagc ggtcacatcg acgcggtcgg
     4921 cttcgagatg tacgtgaagc tgctcgaaga ggcgatccgt cagcttcggg gcgaggagct
     4981 cgaggaagag gcccgggcca ccgtcaacct gcgcatcgac ttgaggatcg acgacagtta
     5041 tatccccgat atgaaccagc ggctgatggt ctatcgaaag gtggcatcag ccaggacgga
     5101 acccgaagtg aattccttgc tggaggaact ccgggatcgc tacggggccc cgcccgagtc
     5161 agtgctcaac ctcgccgagt acggacggac cagaatcatg gcggaccggc tcgaggtcga
     5221 ttcaatcgac cgggagggcc gcatgatcgt cattagattc aggcccaatg cccgactcga
     5281 tggactgcat ctcgtaaagg tcgtgagcgg ctggccgggc gccaccctgg tacctcccgt
     5341 gtccgtcacg ctggatctcg aggctgcgcc cgaaccgtcc gccgaggctc gccgggcggg
     5401 gccgaagagg ggcgacacca gtcgaggtcg tagtcatgcg gcgcagggca gctggtggac
     5461 cgcccgcgcg ttggctggtg aagtgaaagc ggggttcaac aaggccgaag tgctgcgcgt
     5521 gcccgagcgc agcccgaggg ccgaagacgg tttgttctcg cgcctcctgt cgctgcttca
     5581 ggcgctcgac gccgggtgag gcttccggag gcggtcgatg tcatataaaa tgttgatgtt
     5641 caaggaattg tgatgttcaa gcgaactgtc gtcgcgatgg caatggcggc atgtgccgcg
     5701 tctgcaacgg tcggcgccga aatcctcgag caggtacttg tcagagtaaa cggcgaaatc
     5761 atcaccaaga cagagttcga ggctcgccag gtcgccgcgc ttggaaaccg gccggaactg
     5821 gccggcgccg ggccgtcgag tcccgaattg cggaaagcag tcgccgagct cacccccgat
     5881 ctgatcctgt ccgctgtcga cgatctgctc ctggtccagc ggggccggga gtcgggatat
     5941 gccctcggcg atcaacagtt cacgcagatt ctcgagaaca tcaagacgac aaacgacctc
     6001 caggacgacg ccaagttccg ggaagcgctc cggcaggaag gcctgacgct cgccgacctt
     6061 cgacgcaacc tcgagcgcca gatgctcatc tcgcaggttc agcgcgtcga gattctcgac
     6121 aagatcagcc tcactgacga agaagcgaga gcgtattacg agtcgcacct gaaggagttc
     6181 acggcgcctg cagaggtcac gctgcgcgag atcctgttcg aagtcgccaa cacgagccgc
     6241 ggtttcaacg tggctgaaga cgacgcaatc cgggcgcgcg ctgcggaagt ccggaaacgc
     6301 ctgatcggcg gcgagccttt tccgcgcctt gcgggtgagt actcggcgtc aggatcgaaa
     6361 gcaaacggcg gtctcgtcgg ccccttgaat gtcgaggagc tgtcgtcaaa cctccgcgac
     6421 ctgctggccg gcatgcaggt gggtgatctc accgaaccgc tccggacggc gcgcgggtac
     6481 cagctgatca agctcgagtc tcgaaccgac gcgagggtgc ggtccttcga ggccgcgcgc
     6541 ggtgatatcg gcaacaagct cggcgagcag aagctgcgag tcgaacgcga aaagtacttg
     6601 gatcggttgc gggagcaagc cacgatcgtc tggcggaacg cggagctgaa gaaggcctat
     6661 gagaccgcgc tcagccgccg acaagccgcg aacactcagg cggctgcggc cgcgccggcg
     6721 aactgaggtc ggccgagacc gtcgatgagc cagaagtcga cccagcctgt gacggagatc
     6781 ggtccctggc gcgacgacgg ggagaactgg ttcgcattgt ggattcggtc gcgccatgaa
     6841 caggtcgtgc gcgagcagct cgaacagaag cgaatcgaga cgttcctgcc taccgtcacc
     6901 cgttggagcc ggtggaagga ccgcaaaaaa agaatcgact ggcccctgtt cccggggtac
     6961 tgcttcgcgc ggttcgatcc ccgcgaccga ttgccggtgc tgaagtgcac tggcgtcgtc
     7021 aacatcgtgt cgttcgaagg cgaacccgca ccaattccgc agctggagat cgaaggcata
     7081 cgacggctcg tgcagagcga tctggcgtac gatccgtgcc ccatgattcg ggagggcatg
     7141 atggtcgaag tcgttcacgg tcccttgacc ggcgtcgtgg gccggctcgt ccgaaagaac
     7201 gacaaagcgc ggctggtgct ctctgtcgat ctcatcggtc aggcggtgag cgtcgaagtt
     7261 gatgccgccg acgtccggcc gtactgacag ctcgtgaaga tcggctccat cgaattccca
     7321 tcgccgctgg tcgtggcacc aatggccggc atgacagaca cggctttccg ccgcctcgtc
     7381 aagaggcagg gcggctgcgg tctggtggtc acggagatgg tgtcgtcgga gggcctggtg
     7441 cggggaatcg accgcacgct cgagtacgcg gaatacaccg aggaagagcg tcctgtcgcc
     7501 ctccagattt ttggcgggaa tcctcacacg atggctgaag ccgcccgcat cgtcgaaggc
     7561 atgggtgccg atgtcgtcga catcaacatg gggtgcccgg taaagaagat tgccaagcac
     7621 aatgcgggct gcagcctgat gcgggaaccc gaacacgccg cgcgaatcgt gtcggcgatg
     7681 acaacggcgg tgagcatacc cgtcaccgta aagatgcgtg cgggatggag cgacgacgag
     7741 cgcaacgcgc cgacactggc caggatggtt caggacgccg gcgctgcagc cgtgacgatt
     7801 cacgggcgga cggccgagca gtcatacagc ggcctggctg actgggacct ggtcagccgt
     7861 gtcgccgccg acttgaagat tcccgtgctg ggaagcggcg attgcctgga gcccgagcag
     7921 attgtgtccc gactgcaggc gggggtcgcc ggagtgctcg tcggacgcgg cgtgcttcgt
     7981 aacccctgga ttcttgcgca gtcgtccgac atcagagccg gacgcgatcc gcgggcggtc
     8041 acgatggaag accgcggccg gtttctgctc gattacatcg atctgctgat cgaccagcga
     8101 gagaacgagg ccagagggtt ccgtcacacg gccccgggcc cgctgccgga cgcggctcct
     8161 gcgtcgggac gcgagcgctg ggtcatcaac aagattcgcg cgttgtgtgc gtggtactcg
     8221 aaggggctcg atgccggctc gcatctccgc gtgcgagtca acagcgtcga tcgggtggcc
     8281 cagctgcgca gggctatcga ggaatacttc ctggcgaaca catcggtggc gccggcctag
     8341 gaacacgcgg cgaatggatt ccgacgtctc acggcagctg cgccagatcg ccgaaggtct
     8401 ccgggcgatc agcgaagggt cttatgccgc gcaggtggaa cgggcactcg acattctcga
     8461 gaccgcgttt cgtgccggcg ggaccctgct ggtctttggc aacggcggat ctgctgccga
     8521 cgccgagcac ctatgcgcgg aattggtcgg tcgcttctcg gccgaccgcc gcccgttgcc
     8581 ggcgatcgcg ttgacgacca atcaggcgtt cctgacggcg tggagcaacg accggcaatt
     8641 cgatgacgtc ttcgcccgtc aaatcgaggg cgttggacga cccggcgacg tggcgtgggg
     8701 catttcgacg agcggtaatt ccccgaacgt cgtcaacggc ctccgccggg cccgcgagct
     8761 ggggctgagg acgctgggac tgtcgggagc caccggcggg cccatggccg agtggtgcga
     8821 tgccctcatg gcggttccac tcgaccagac gccgcggatt caggaagcgc acctcgtcac
     8881 ataccacacg atttgcggcg agctcgagcg ccggctgttc acccggggct gagcgtgtcg
     8941 ggcggctagt gggccctgcg gcgagccagc gagcggacga gcgagaccat ctggccgcgg
     9001 tcgcttgctt gagcgaccgc ttcgcgcagt gcgacgtggc gtcgcgcgac ggcccgcgcg
     9061 agggattcgt caacagaacg catcccgggt ccgccgtcct gttgcatgcg cgcgagcctg
     9121 tcgaagtcgc ctgcctccag cgccccgcac accgcgctgg tcgccagcac gacgtcctga
     9181 accagcgtcc gtcctccgcc gatgcgacga aggctgcgat agccgattgc ggctctgaac
     9241 gaggcggcca ttgcgcggcg aacatgaacg tcggaaccgg acagcatgcg caacgcatcc
     9301 accgtggtcg atgcgacgac gccgacgatg accagccgcc cgcccgccgc cgccaggacg
     9361 gctgactgaa gcgcctgttc ggactggagc ccggtcacga gaaggatatc gggagcttcc
     9421 tgtgaggcgc ggcggatggc ggcagcgaag tctttctcgc ttccgctgat cgttcgccgg
     9481 ctcacgaaca cgcctgcgat gtcctcgaga gagcggctgc ggcgctgaag cgagatcagg
     9541 tacccgccgc ggtcgcgact gctccaatca gccacggctg ccgccagcat ctcgacatcg
     9601 gtctcgttcc ccgccgccac gaccacgaga ccctgcgcct cgcaggccag gcggatctga
     9661 cgcgggatgt gcttgtgcaa gaggcgcagc gacgttgtcg gtctgagctg aacgacgagc
     9721 cccgtgccgt ccaggtcgct gaagacgcgg cagctgacgt agccgaactc ctcgtcttcg
     9781 cggacccact caccgtccgg gcgcggctgc catcggccgt cgccgccacg agagaagatg
     9841 tttgcgacct cgtccaccac cgacgcatcg acgatctcgt cgctgacggg ctgaatgcga
     9901 tcgtcgattc gggccgcagc attcgaaccg gcgcggagaa acatcatgct cgcgccgaga
     9961 tcggctgcct ggcgaatcca cgcggcaagg tcccgcggcg gttcagggcg ggaccgcgac
    10021 tgtcgaacag gttccggggt gtaagacgga gcgggtcgct ctatgtgcct gacaggttcc
    10081 ggcggcgcga acggccttgc gtcgaagcgc acctcaggcg ccggtgcgac tgcgatcgga
    10141 ggcgcggcga cagcttcggg ttcggcaggc acgaagaccg gcggatgttc gacgaccgga
    10201 atcgctggtg cgggttccag aacagaagcc gcgttgtcag gaagcgagac gatcaactcg
    10261 atcgaaaaat cgtccccgac gcgctcggct cgggcagtga gcggatgggg aaaagactcc
    10321 gaatgcaccg gttcgctgac cacgccgttg gcactcagtt cctgacgacc ggcaccagac
    10381 agaatctgtt ccgccagcgc ttcgacagcg ttgatagaga ggaccgcaga ggcgacgtcc
    10441 tggcggcgcg caccggcgag tacgtgcggg cgttcccccg ctctcatcac gaggcgttca
    10501 ccacctgcgc gttcgagtgc tgcgcagagc gacggcatca ggctcatagg tcagcgtcct
    10561 gcagagtaag cgtgtcttcg aattgcagcg ccgcccgaaa aaccggagtc ggcgcgaccg
    10621 catgcatcga cgaacgtatg accgttgcgc ggagaacctt gcgcaccccg ccgtagcgaa
    10681 ggaagacgtc cacggttcga cccggacaca gtctctgact cgtttcggcc agaagaccgg
    10741 tagacgatag attcacgagc cgcatcggaa aaccggcgac ggccagccgt atctcgactt
    10801 tgaactgatg tggttcgcgc cgtggggcgc cgcgtcgttc cttgcgctga ggcggaggtt
    10861 ccggaaccgg atccggcttt gcctctgcgg gggactgagc tttggccgcg gcagggctct
    10921 gccaggacgc ctccagaccg gcgaaaaggt cgtccagaga cgccttctcg ggaagcttga
    10981 cgaccatctc gtcgtcggaa tcagagcccg gatcgctcat gaccgcacaa agtgcaacct
    11041 ggctgccggc ccctcgagac gtttcttgcg cagggcgatg tcagagttgc attagaattc
    11101 acagtaattc ccttgagtaa cgacctatca taggcgaacg atgactgagt cgtcctccca
    11161 atccttgaag ttccggatga ctcgcggacg agaggggaga ggccgtcaat ggcgaagatc
    11221 ctagtcgtcg aagacgacga tgcgacgcgg gccggctacc gggaactcct gagccgggca
    11281 gggcatgagg ttgtcgccac ggctacctac caggaagggc ggcacgccgt cgctaccgag
    11341 agcccagacc tcgtgatcgc ggacttgcgg ctgggtggat tcaacggact ccagttattg
    11401 ctgctcagcc cgcagccgat cccgacgatc atcgtgaccg ggtttcatga tcaggttctc
    11461 gaggctgagg ccaggcgcgc gggggccgat tacgtcgtca aacctgtctc cccctccaac
    11521 ctcatgaccc tggtccgcga aaggctcgag agtcaggctg catctggacg ctgacaaatc
    11581 tgcgcgcggg aacccgctac cctgagcgcc acgggagagt tcgcttccac gcgacatcga
    11641 gaacgatgac gcctccaagc gttagtcacc cggaacgcaa cctttctccg tctcaaacgc
    11701 gccaatcccc tttcgtttgc ggccttcttg ggccaatctt gtgctcttac tggcgactgg
    11761 cttgcaggag tcgaacggcc acttcccagc gctgaatgtc gagcatcaag agtcagttac
    11821 cggtcgcaca ggaaccgacc ctcttcgagg gagcggtccg tgaccgctcg tgggaatggc
    11881 tgctcgaact gcgccgccgg ctcaaggtcg aactgctgtt ggtggatgga cgctgttcgg
    11941 tactgctgcc cgcgcgggat caggcggccc gccttcaggg tctgctcgaa caaactgaac
    12001 cggtcctggt gtctgccatc aacgcgacgc tgcgcagcag gacagcccac gtcgtggagc
    12061 ttggcgggct tcaggcagtc tgcctggcga tatcacgtga tcggggagac gccggggcac
    12121 tactcgtcgg caggaccgtt ccgccggggc aggacccacg ggcctcccgc gatcagctcc
    12181 aactggttac ctcgtggttg gccacggccg tcgaggcgca ccttctcagc ccgccgtcgc
    12241 ttctttcgag tggcttgagt cgagtcgctc cccttgccca ggtgttcggc cagtgcgcgg
    12301 aacacgaatc cgatcgcgag ctcgttcgac tgtttggcga ggcggtcgcc gtctggcacg
    12361 acatcgaagt ttgcgggtac gtggagacgt ccaatggcgg cttcgccaga gatgtgacac
    12421 tgcccggcgc gaacaagggc gaaaggcccc tgtcgatttc cgccgaggag ttgcccgagg
    12481 cgaccgaact cacgcgactt cctcacgggc atctcgaccg attcggactc ccggtgagtt
    12541 gcgacgttta cgtgagccgg ttggcccggg ctgcggagcg atcgtggctc ctcgtcttca
    12601 tcggagccat tgacgactac gatcttcagc gcctcggggc gtatctggcg ttgctggagt
    12661 tagcccttgc cttgtcgacg tcgacgatga ccacccgcgt ggtgaacacg atcggcagca
    12721 ggctcagcga cgttcagcag acgccggaga tgcgcaccag gaatgcacta gaggagctcc
    12781 gccgaacgct cggcgcgtct tcggccggtc tcaagatcga gtcgccagac ggggcgccct
    12841 tgttcgacgc gctgagcccc gaccttgccg acacgtctcg cgcggaccca ggtgcgtcga
    12901 gactagtgct cgtcaaacgg tccgaacagc attacactac gaccgtttcg ctcggtcgct
    12961 atgagagtcg gcagttcact ccacgagact acgccgtggc gagcgctgcg gcgaccgtat
    13021 tcgggctctg ggttcctgcg acgttgagca ccgctgcggg tcggcgcgaa cgccgggccc
    13081 tgccgcgcgg gtttcaagaa gtaattgaac ggtcggcctg cgaggcaatc gaacgaggtg
    13141 tgcccgtcgc agtcgttgtt ctgctgattc gagatgccat atcgctgccc gggtccaccc
    13201 agcgttgggt ggcgggcatt cggggtcaaa tgagggcgtc ggacgtggcc gggatgctga
    13261 cagaaggaga aataggactc ctgttgcacg acatcgccaa cgagcaggcc aaggccatcg
    13321 ccgaacgcct caggacggtg gtgagcggtg cgcccggacg ggaggcgatt ctgatcggtg
    13381 tggccagttg gacgccaggc caggacccgg ccgatggtat tgttcgcagg gcccgtgcag
    13441 atgcactcgc cgacggtcgg cgtgctcaag tgtccgtttc acctcgcgga gtgaacagct
    13501 gatggcgttg ggggatcgac gttctcgcct gcgccttgaa gtcgtcggcg cgttgtgggc
    13561 cacgttggag ctgacagagc ccgcccgggt ggtgaatatc agccgggatg gggcgctgat
    13621 cacgtcaccc gtcccaatga caccagagtc ggtccagccg cttcatctga agctggaggg
    13681 acgagaggtt accgtggatg cacgcgttcg ccacatccgt cgtgtgtccg gaccggacga
    13741 gcccatccgg tacctcgttg gagtcgagtt tcttacagtc cctcccgcac tcgcccgcgc
    13801 cacggaatgg ccgtcgtgag acgccaaaat cgtgtgaggg aaagtctacc gcctagagtg
    13861 tcttagcccg gacttgctca gactttctga ggggcagtct cgagatcaga gtcttcgcgg
    13921 gccagttcat aaacatcaga atataaaaga cttgcgtcgg cagacaaacg tctgcttgac
    13981 gatggcgact cacttgcagc ctcggcagac ttcgggaaac ccgtaggaca tgaacgagtc
    14041 cgcgcggtgt gcgcgaggag accgatggag gaacggcggc ggaccccacg gcagcaggtc
    14101 ggtgaggaat tcgcaaatct gccagggaac atgagcgtcc gggttctgga catcagcacc
    14161 gtcggcgtgc tgattcagtc cccccgcctg ctcaacccgg gatcacgcgg aagtctgcgc
    14221 ttcacgcttg ccggagcacc ctttgcggca gacattcaag ttcggcgcgt gtcctcggcg
    14281 tccgacaacg gatccgggta ccgcattggt gcgacattcg tgggaatgac catggatcac
    14341 cgacagctga ttgaacggtt catgaacaat gattgatggt gtagctgaaa gcagcactcg
    14401 agtcggcgcg attggcccga ttggggcgat tcggccccga tctcacgtcc agctcactgg
    14461 ccgcagcgcc gaggttgtcg cactcgaaca agagatcgac cgggtcgccc gctccgacgc
    14521 caaggttctg atcacgggcg aaagcggtgt cggcaaggag atcgtcgcac gcgcgattca
    14581 ctcgcgaggg cctcggtccg gacacgtgtt tgcgccggtc aactgcgccg gtcttcccga
    14641 aacgctgctt gaatccgaac tgttcgggca cgtcaagggc agcttcacgg gggcctaccg
    14701 cgacaagcct ggcaagttgg aaacggctca catgggtacg atcttcctcg acgaagtcgg
    14761 ggagatgacg ctccggatgc agggcttgct tctgcgcttc ctggagacgg gtgagcttca
    14821 gaaggttgga gccgatggga gcggccgcat cgtcaacgtt cgcgtcatcg cggcgaccaa
    14881 ccgtgatctg cgcgacatga tcaagcaggg aaccttcaga gaggacctgt tctatcggct
    14941 caacgtcatc cacatcgttg tgccaccgct tcgggaccgt cgcgaggata tcccggtact
    15001 cgcgaaccag ttcctgtccc acttcacgac tgccgggcga tccacgatca cggggatttc
    15061 ctcggatgcg atgaaggccc tgatcgagta ctcgtggccg ggtaatgtgc gcgaactcga
    15121 gaacgtcatc gagcgcctcg tcgtcaccgt cagcggtccg acgatcgaga ccgagcatct
    15181 ctcatcggag atcagagcct acgatgccgt aatgctccgg cccaagcggg agcgacggcg
    15241 caccgtggcc gacgatctgt acaagcgcat gctcgagcaa cacgaatcgt tctggacggc
    15301 cgtgtacccg ctgttcatgg accgcgagat tacgcgagcg aacttgcgcg aggtcgtccg
    15361 ccgcggcctc gaggaagcgc ggggcaacta caagatcgtg gcccgcctct tcaacatgga
    15421 ggcgcgcgat tacaagcggt tcctgaactt cctccggaaa cacgactgcc agatcccctt
    15481 caaggaatac cgctgaccgg acctcctcga cggctcgaca acctcgggcc gtcgcgggcc
    15541 catcgcacac caccggtttc gccaaggccc cggtttgccg cagcggggac gccggaggca
    15601 cggttgttgc caactcatca gggctgtgtg gccgagcctg tccgaaggtg tttcgacaca
    15661 agggattagc cagaccctcc gaggagtctc gtgacaaggg actcccaaag agggacccaa
    15721 tttctgcctg acggaggact tcatcggctg atgccgtctc atgaagccgg ccctggcagt
    15781 cgtcacctgt ccgaagggca gcgccagttg ctcctcgagc tgcacctcaa aatcgaccgt
    15841 acgtccccac cgcgtgaatt cctccgtggg ctcgtcaacg caatcagcga agccctgcag
    15901 acgaccgtag cagtggtcgg acggaacgga gatgtctggg tgcgagcggc cgcgtcgggc
    15961 cccgccaccc cgcgtctgcc acttcagtcc gagggttggc ctcagatctt cgaccgcctg
    16021 agcgcgacgg caggcgcaca gctggagctc tggcgcaccg gaagtgagga ttggaccttg
    16081 ttcggactgg caagggcgcc ccatacgccg gtgattctca ttctcgaagg ggactggacg
    16141 cattgggccg ctgtccttca acagatggca caaggtctgt cgatcgggga tgtccgtgac
    16201 ctctcgcaag cacggccgct cgacagcatc ctccagcgac tgtcgcacgc actcgccgac
    16261 actgtggggt tggccgaagt cggcgaagtc gtgcttcggc atgtgattca agccgtgccg
    16321 agtcggctgg cttccttggc gtccgcagcc cccgacggcg aactctcaat cgtggccacc
    16381 cagggctatc cgcgcgaact ggtccagcac cttcgaatcg cgtccggcaa cggcgtgata
    16441 ggagcagtcc acctctccag gacaccgttg gtcgtctcag acgtgtcgct gaccccgggc
    16501 cttgaccgtc atcggtcgag gtatcgatcc aactcgtttg tggcggttcc aatcgtagcg
    16561 gggtccgaaa cgcttggcgt cgtatcgctg accgaccgag tcgcctcggg cccctatacg
    16621 cagggagacg tcgcggcaat caccgtgttg atgggtccgg ctgcgcttgc gctggcgcgc
    16681 gaacgcgtca gacaggaggc cctcgcctac gcgcaggcag ccgtcgtcga cccagtgagt
    16741 ggcctgttca accgccggta tttcgaggcg cgtctcgacg aggaattaca ccgcgcggtt
    16801 cgtcagctca cgtccgtggc gctcttgatg gtagacctgg atgagttcaa ggcggtgaac
    16861 gatcgcttcg gacacatcgc cggcgacctg gtgattcggg acatttcgga gattcttcgc
    16921 cggtcggtgc gtattttcga tgtctgcacg cggttcgggg gcgaggagtt cgcggtgatg
    16981 atgcccggcg gcaccgttga aagcgcaagc gcaattgccg agcgcatccg ccatagggtt
    17041 gaggcttatc agcggaacga gccgagcttg gcgggactac acgtcaccgc gagtatcggc
    17101 gtcgcggtgt ctccccccgg tgttactgcc cgagacctta tggaacgggc agatcgtgcg
    17161 ctctaccatg cgaagaaggc tggcaagaat cgcgtcagta ccgccggcac agacccggtc
    17221 tgacctgcct ggtctgacct gcccagattc cgaatccctc agaaaatcag caaacgcggg
    17281 tgcccccccc aacaaatcga cctgccggcc tcagaaatcc acctttcgtg gcatgactga
    17341 cgacagatcg tggcgacgcc gatccgagca gacttgtgca aaaagtcaga agttcgatca
    17401 ggccccccaa gacgtttaat actgtaattg tctctagatc accgaattag cgcaaaagcg
    17461 tgatctttag gacgcttttc gaccacttct cagttgacct tagtctcgtt aaaaaggcag
    17521 acttattccc tctgaattag caaagaacct tttccctctg aattagcaaa gaactttgga
    17581 gcttatcccg aatgtcgaca ctttctctac cccgaatgcc gaaaaacctg ccactgctgc
    17641 tcggcgagag tccagccatc agttctctgc gacgtgaaat cgaggcagcg gccaggaccg
    17701 acgcgaaagt cctcgttctc ggcgaaacgg gatcgggcaa ggaagtcgtc gcgcgactga
    17761 ttcacgagca gagtggacgt tcgtcgcgcg cgtttgtcgc tgtcaactgc agcggcatcc
    17821 ctgagaccct cctcgaatcc gagctgttcg gtcacacacg aggcagcttc acgggcgctt
    17881 accgcgacaa gccagggctg gtgcggcaag ccgacagagg cacgctgttc ctcgacgagc
    17941 tcggagagat gagtctcagg atgcaggcag tcctgctgag attcgccgag accggcgaga
    18001 tccagacgat cggcgtcgac ggtgtgacgg gtcgttccga cgtgcgcctc atcacggcga
    18061 cgaaccgaaa ccttcgcgag cggatcgatg ctggcgtgtt ccgcgaggac ctgtacttca
    18121 ggctcaacat cattcagatt catgtcccgc ccctgcgcga acgaaccgag gacgtgatgc
    18181 tgctgctgcg tcattacctg cgcagagcca gcgagacgca tcgcctcgcg tatccggagt
    18241 tgacgctgga cgccgaacag gtgctcgcct cgtatgcctg gccaggcaac gttcgcgaac
    18301 tgaagaacct cgcggagcgc ttggtcgtgc gcgagtggga tcgcgccatt ggcgccaacg
    18361 atcttcctgc cgagatccgt ggagggcgcg acactgtctc gacctcgccg agctccagcg
    18421 tgacgttcaa tcaggacagc gtgcctaccg cgcgacggct cgagcgaggc tccggaacag
    18481 ccgatcgctt gtgggaacgt ctgatcgcgg gcgagagctt ctggaccgtt gtacaccagc
    18541 cgttcaaagc gcacgaactc acgcgggatg acctcgccgc tttgattcat cgcgggctcg
    18601 agcacacgag aggaagctat cgtgctctgc tcggcgtgtt cggtctcccg cccacggact
    18661 acaagcgctt ccacgcgttc ttgtatcagc agaactgcaa cttgcctgtt ggaccgtatc
    18721 gcaccgcgag agcaacactg cgccggcaag atcggcccgc gtcggtgtac acaccggcct
    18781 gctgatcgtt ggcagtaaat caggagcagc gttggggcgt cggtcgcccc acgctggttc
    18841 cgcctgatca gtcctcccgc agaagcgcgt tcagcgcttg atcttccccg gtggtgtcga
    18901 aatcgtagat gagggcctgc tcgaaacacg cgaggaaacg agcccctagt tgctggtgtg
    18961 ccgagtgatc cagatacgca cgcagcccgg cgacgtcgtc gaactccaga aacgcggcgt
    19021 gcgtgtagtc gtccttcatc agttgctcgt aggacctccc gagcagcgtc cgccggccga
    19081 ttcgcgcccg cttgatggac gggatttcct tgattgcggt tgagaacgcg tcaacgagag
    19141 cccgccggtc ggcggtggac agtcgggcct tcggcctgaa caaaacggcg tgaacaatca
    19201 tcgacgagac ccttttccgg gccgccggtc gcgtccgcta ctgctcctcg gagcgggccg
    19261 tgatgggtca gccggtgtgg gattgacgtc ccgcgccgca tgcttgcgct cgaagcgaag
    19321 ctcgcggcgc ttctgatttc gagcgcgcct cgtcgcctcg accttctctc gtgggtcacg
    19381 atgttctccg ccgggccgcc attcccggcc gcgtctgagc gccgtcgccg acagcgacgc
    19441 ttcacgcgcg gcttcctccg aaggccagag ccttcctcga gcgaaccatt tcatgacggc
    19501 gtcggggtgg cggtcctgca ggcgcttaag cgtcggcagg agatcatctt tggaagccgc
    19561 gcgaaaggag gttggcagcc ccccggcgag gatcgtccag tacgcgtatc gtggcggcat
    19621 tcgtttctct cgctgctccg cgatcagtcg ggtccgatcc tcttcaactt ctccaccagc
    19681 gtcgtcctct tgatgcgcag caactcggcg gcgcgattcc gatttccacc ggtgcggtcg
    19741 agtgcgcgcc cgatcaagtc acgctcgacg gcggcgaggt aggccggcat gtccatccct
    19801 ccttccggaa agtccacgag gggcatggcc gttgccgggg gggtcacctg gatttcggca
    19861 ggcaggtccg ccatttcaat atgctgccgt cccgcgctca gggccaccgc ccgctcgaca
    19921 gcgttttcca gctgacggac gttcccgggc cactgatagg ccatcaacgc acgcatcgca
    19981 tcctgcgata cctgcatctg cgacccgggc gcaaacttct gcaggaagtg ctgcgcgaga
    20041 accgggatgt catcgcgcct gtcccgcaat ggtggcaagt gaatgggaat cacgtgaagt
    20101 cggtagtaga gatcttcccg gaacgttcct tcggcgacca ttcgcgcaag gtcagaattg
    20161 gtggctgcga tgacccgcac atccaccttg atcgtctggt tgtcaccgac ccgctcgaac
    20221 tcgcgctcct ggagtgcgcg gagcagcttc atctgaagag cggtactcat ggtcccgact
    20281 tcgtcgagga agagcgtccc cttgtgggcc tgctcgaagc gtccctgacg tgcgccgatc
    20341 gcgcccgtga aggccccgcg cacgtgcccg aagagttcag cctcgagcag agtctcgggt
    20401 atcgcgctgc agttcaaggc gacgaatcga tgtgcgcggc gagggctgtt gtggtgaatc
    20461 gctctcgcca cgacctcctt gccggttccc gtctctccgg tgatcaggat cgtgctgctg
    20521 gatcgcgcca ccgtctccag aagatgaaac agcttctgca ttggccggct gcggccgagg
    20581 atgccgccga actggtaacg gtcctcgagc tgtgaacgga ggtacgcgtt ttccgatcgg
    20641 aggcgccgat cttcaagcgc cttgtgcagc acgtgcgaga gctcgtcgaa ctgaaagggc
    20701 ttggcgatga aatccgatgc gccccgcttg atggcgtcga ccgcgtcctt gacggtgccg
    20761 aagcctgtaa tgacgatggc cacgatgccg gggtagcgct ctcgagcgga ctcgataacc
    20821 ctggacccat tgactccggg cagcctcaag tccgtaatca ccacgtcgaa cgcaaacttc
    20881 tccaggagct ccagcgcccg ttctccgctt tcggcctgaa cgacctcgaa gccacgctcg
    20941 gtcaggcgtt cggccatgac cccgcgaagc gcggtttcgt cgtccacgaa caggatatgg
    21001 gggatggttc cggcggcagc cacagcgcca atatcttgac acagccccaa actaaggatc
    21061 ggtcagggag cggccgatac agcagtcgaa cgagctcggc gggtcaaagt ggtccgccgg
    21121 cgttactaac ggccgtctcc aggaggcccg tgagactata tggaccgcca gaccctctca
    21181 atcatgaagg cgtgtgaagc cgtcggagtc agccggcgaa ccatttacaa ctggatttct
    21241 gccggcaagg tcgaatacgt ccggacagcc gggggctcga ttcgcatctt cgccgacacc
    21301 ctgtggcgcg acgcagcgac gccggcccgg acccaggaat cggattctcg aacggcgcct
    21361 ggacagcacg cgtgagcgtc cctcaggggg tcgatggcgt cggcgacgtg ccggctaacc
    21421 tcgaccttca ggcgttgttt catcgcctga acaatcagct cgggtctctc ctcgcccacg
    21481 ccgagctcct cgaaaccaag gcccctgacg acgtcaatcg ggcgcgcgcg gcccaggtcg
    21541 tcaggagtac cctcgatgcg atgggcaccg ttcgagcgat ttatcatcaa acggaccctt
    21601 ccggcgagta gccggctcgt ttcagcacat cgacggcgag ccctgattca ttcctgagaa
    21661 tctactcaga tccgtaattc tgctgtcatc aacttggcgg ggacgttgtt gtttccgtca
    21721 atctgttggc gattttagcg gtcttttgcc cgattggaag ctcgacagaa tcgtcagacg
    21781 agcactggta agtggctgtc ctgatagtca cttagctcgg gttttctcac gagattgcac
    21841 tgggatgcgc gccgattcgc cccagttggt accacggttg cctcatgtgg cgccggaggt
    21901 cacattcatg cgcccccaga tcaactccga gaacccgagg gttctcgcca gcatcccctt
    21961 cgtggagcag ctcgcccggc gagtcgccgc caccatgccc cattccatcg acatcggcga
    22021 cctggtgcag gacggggtca ttgggctcat cgacgccgcg catcgcttcg acgaggcgag
    22081 aggcatcaag ttcgaaactt tcgcggaacg ccgcattcgc ggcgccatga ttgacgcact
    22141 acggaaggat gcctggcccc gcggagtccg acggcaacgc cgcgagctcg aagccgcgcg
    22201 cgaggagctc cggagagagc tggggcatga accgtcgctg gcggatctgg cggccaaggt
    22261 cgggtcggac gaaaaacggc tgggcaggac gattgtgcga atcaacgcca tcgaatcgac
    22321 ctctccactt gccaacaacg atcacctcga cgagtcgacg ctgcccaccg cgttgatacc
    22381 ttcagagccc gaacagccgg acgccgcgta cgagcgacag gaaacgaagg accgcgtgtg
    22441 cgcggccatc gcttcgcttc cgccgcgcga gcagaaggtg atcggcctct actactacgg
    22501 cgaagccacg atgaagcaga tcggcgctga aatcggcgtg aacgagtccc gcgtgtcgca
    22561 gctgcatgcg cgcgccatca gacggcttcg cgatgcgctc ggcacgctgg tgcccggagc
    22621 cgaggtcgcc aaggtcatga ggagcgcgat cctcgagttc cagcagaaac cgcgtatggc
    22681 caaggcgaca ttggacagcc gccaggcggt tgccgggaca gaacggcttg gcgtcgtggt
    22741 caactacgcc agcgcttcac gaacgttgcg cagcaagtcg ccgagccgga agggtttggc
    22801 cagccagcgg cagccgctct cctcgagaaa gcgctccgcg tcggctcctg ccacgtcgcc
    22861 ggtcacaaag atgacgcgcc gggcgagcgc cggcgtcgct gcggcaatcg cgcgatagag
    22921 cgtgatccca tcgaccttgg gcatcttcag atcgcagatg accaggtcgt agctgcgctg
    22981 atgcacgcgg gcgagcgctt cctcgccatc gcccgcgtga tcgactctca atcctgcatc
    23041 gatcaacgcc tcgctgacgg ccatggcgag cgcgcgctcg tcctccacca gcagtaccga
    23101 ggctctttgt gtcaactcgc tcctggaggc cgccgcgcct ggggctcgcg tcgtcgggtc
    23161 gactgagccg gccggcagtt ccacggtgaa cgacgcgcca ccagtcgcgg gcgagtcgac
    23221 ccgaatgctg cctccgtgtt cctgaacgat ggcgtacgcc acggtcagtc cgagtccagt
    23281 gcccttacct gtttccttcg tggtgaagaa gggatcgaag atcttgctct tgacgtccac
    23341 aggtattcct gggccgtcgt ctgtgacctg gagcacgacc gcatctcgct cgaggtcgtg
    23401 ccacgttcga agcaccaggg agcctcggcc atgggcggcc atcatcgcct gctcggcgtt
    23461 gatgaccagg ttcaaaagca cctgctgaag ctggtgcgcg tctgccagca ccttcggcag
    23521 gccggaagcc agagccgtga tgacctcgat gtgacgacga tgctcgtacg agcgaagcgc
    23581 cagagtctcg cgcacgacct ggttgacgtc gaggagcgaa cgcgtggact gacgtttgcg
    23641 agcgaaggtc agcaggttcc ggacgatgcg cgcggcccgc tccgactcgc cgagaatcac
    23701 gtcgaggcca cgacgagtcg tttcgtcgga cgctttttca gcgagccttt cagcccagct
    23761 gagaatggtc gccagcgggt tgttcaactc gtgcgcgacc cccgagatcg tctgaccgag
    23821 ggcagccatt ttctccgcct ggagcaactg ctgatagagg tcgcgcgatt gctcgtcgag
    23881 ccgcttccgc tcgctgacat cacgaacgag cgcttcgact cgaacacgcg tcccggagcg
    23941 gtcccggtcg gtccgggcag tcacctcgac ccaaatcgag tcgccgtcgt gccggcggag
    24001 gcgcagcaga tagtccgtga ccgccccatc gttcaagagc cgatcgagaa acgcggttcg
    24061 cgccgtcgct tcgacgaagc tctccggttc gaaggggagg acgttctggt ccggtgtgtc
    24121 ggcggggaaa cccaggatcg acttgaggtg cggattcgcc gccagagttt ccccgggccg
    24181 atcatcggcg ctgtcgatcg ccccgatgta cacgccctcg tgcacagctt cgaagagccg
    24241 cgcgaaagcg ttcgccctct ggccgtctgg gacacgccgc ggtactgccg ccacgacgct
    24301 atagattata ggcgtcccgc aggtcgattc tgcgaacgcc aggcacgaat ctctcttagt
    24361 gctgcttcgc gtcgtgcgcc gggcgcggtg tcggcgtcga cgaagggaag gacgagccgt
    24421 acttgcgatc gatcgccgca cgaatctgcg acggcgacgc ccccgtgttg aagagcgcga
    24481 gggcatcgag gccgacatcg atgcagatcg cgcagcccat gccatggctg tcccacgcgg
    24541 tcactgttcc attggcggcg cgcgacttca cgaagcaatc atggtttccg ttgtgaccga
    24601 cgcgctcgca tccgcagtag cacggaacgt atttcagcac ttccggatgt cgcgcggcga
    24661 actcgtacac ctgacgcacg gtcgccatcg gacggggcgg cgtgaacggt accgacggaa
    24721 tggccggcag cggtccgctg tgcgccttca ccgtggccgg cggcgagggc gcgcgggagg
    24781 tctgcgcgta ggtgtgctgg acgaggccga ttcctgccga gacgagcaga accgtgcaca
    24841 gtgtcagtgt caggcccctt gtcgccgttg gtctgaaagg catgtgacat cctaacccga
    24901 tccccagctc atccgcatgg agttccgaca cttccgcgcc gatacgccct agaaggcgag
    24961 gagcgaggac ccatgcgatt ggccgggcgc aacgacgttg tcctgatggt gggactgacg
    25021 gtcgccctgt ttgtcatctt ctcccagccg gtctcgcggc tgctcgacta catccgcgag
    25081 atcgaggcgg cccgggggct tcacctgctg ccggccctct tcatcctcgc gaccgtgttc
    25141 agcttccacc agatccggaa gcggcaggag atgcgcgagg aggcccgcgc cgtggccgcc
    25201 gacgccaaca gcgcgaccga gcgcgcgtcc gagatgtcga ggctcgtagc cttcggccat
    25261 gcgctggcgc gtgcgatgga cagcgactcg agcgactcga ttcgcgacgt cgccgtcgcc
    25321 cacattccgt tgctcgtccc cggcaggaat acctgggtga tgacccgcgt gcggagcggc
    25381 cattgggaat cgctcgtggc catcggggaa tcgtcgccca tcgaacgcga gcgcgccgcg
    25441 cgccgcgcgc tcggggaagc cgagccgctc gtcggactgg ccccgactga aatctgcttc
    25501 ccgatgatcg tcgcggggac gccggaaggc gtcctcggcg tcgcgccgga gcctccgctc
    25561 accaaccatc agcgaagcgt gctgagcgcc gccgccgcgc tgctcgcggt gtcgctcaag
    25621 aactcccaac tgctccgcga agtgcgcgag aacagcgttc gggacggtct cacgagttgc
    25681 ttcaaccggg gccacgcact cgaagtgctc gacgccgaga tcaggcgcgc gaggcgttcc
    25741 aagctgccgc tctcgctcgt catgttcgat ctcgacggtt tcaaggaggt caacgatcag
    25801 tacggtcacc tctgcggcga cgccgtgctc gcatccgttg gaacgaagat gaagtcggag
    25861 ctgcggggaa gcgatctgaa gtgccggtgg ggcggcgacg agttcatggt catcctgccg
    25921 gacacgccac tcggcggagc acgacaggtg tcagagaatc tgcgccggca gatcgaaacg
    25981 cagaccgtcg cgtggaacga cgactacgtg aaggtgactg ccagcttcgg gatcaccgtg
    26041 atcaacccgg ccgatcagga tcccctgacc gcggtcgcgc gcgccgacgc cgcgttgtat
    26101 cgagccaagg aacgcgggcg gaacaacgtc caggtccagg agcagccggc cgccctcgtc
    26161 tagggggcgc cctcgccgga cgtccgggga gccggtccga gcgcgaacgt catcgtccca
    26221 tccacgacga cgcgtccgtt cacgcgcgcg tatccgtgaa gctgaccgac gcggctccgg
    26281 aggctcttga cccgagcttc gatctcgacc acgtcccccg catagacctg ccgtctgaac
    26341 cgcacgcgtt cgatccccct gaacagcggg atccttcccc ggttctccgg tttcgtgagg
    26401 atgagtatgg cgccgacctg tgcgatgcac tcggtcagaa tcgtcggggg cagcgacgcc
    26461 gcttcgcccg ggtgatgcgc gagacagcgt tcgttgagcg agacgttctt cacgcccacg
    26521 atccgcttgt ccggctcgag ctccgtgatg cggtccacga gcagaaacgg atagcgatgc
    26581 ggcaggatgc gctcgatggc gctgtagtcg aggggtagcg tcagagggtc catcgcgaat
    26641 tgtcagtata attcgttcca ccctcgccaa gcgtttcgta cgtccgcatc tcccatgagt
    26701 gaccaggtcc cgcacccgcc tcagcccgtc gcacgcgatc aaggaagtgg cggccgcgtc
    26761 cccactgata ccgagctcct ctcgacgctg ttcgacctcg ggcgggaggt gacatcggtg
    26821 ctggacctcg aggagctgct ggccaagatt ccgcaaatca tcgcgcgcct cacgagattc
    26881 agcgcgttct ccgtgtatct actcgacgag aagcggcagg agctccgcat cgcgtacgcg
    26941 gtcggctacc cggaggaggc ctcgaacctg cgcctccccg tcggccaggg tgtcgtcggt
    27001 gccgctgtcg ccgaaggccg cccgattctc gtcaacgaca tacgccagga gccgcgctac
    27061 cggggaccgc tccgcaacat gctctcgcag ctcgccgtgc cgcttcgccg caagggcagg
    27121 gtgatcggcg ccctcaatct gctcaacgaa gccgaagggg cattcagtac gcaggatgaa
    27181 gcgctgctgc ggcagttcgg ggcgcacgtc gcagtcgcga tcgagaacgc ccggctgttc
    27241 aagtccgagc gccaatacaa cgacacgctc gagacgctcg ccgaaatcgg ccgcgatttg
    27301 tcgtcgattc tcgatctcga cgcgctgctg acacggatcg ccagcctgat gaagcggctc
    27361 atcgactacc gcacattcgg aatccttctg ctcaacgagg actccggcga gctcgagatg
    27421 aagttcgccg ttcgttacgg gcagggcgcc gagtcgaagc acatcaagct cggcgaaggc
    27481 ctcgtcggat gggcagcgct gcacaaggag ccggtcctgg ttgccgatgt ctccaaggac
    27541 cctcgctaca tcaacgtcgt cgccgacgta cggtcggagc tcgtgctccc gatgctcgtc
    27601 aaggatcgct gcatcggtgt gttcgacctc gagagccccg agctcgacgc attcagcaag
    27661 gagcatcagg aactgctgac gctgctcgcc ggccaggcgg cgattgccat cgacaacgcg
    27721 cggctttacg aggaagttcg acacaacgaa gagcggatcg agcgcgagct ccggttcgcc
    27781 cggcgcgtcc agcacgcgct cctgccggtc gggccgccga agaccctgcg ggaagccgac
    27841 gtcgcggcgc gctacgagtc ggcgcacgag ctcggagggg acattcacga tttcttggcg
    27901 ccgaagcgcg acgcgctggt cgtcgccgtt ggcgacgtct ccggcaaggg cgcgccggcg
    27961 gccttgtacg gaatgttcgc ggctgaactg gttcgttcgc acacgatggt tcgccgcttc
    28021 tcgcatgaac gcttcagtgt ctcgggagtg ctgaaggcca tcaacaccct gctgtacgag
    28081 cggcagctcg aggcgtacta ctgcacgctg tgctacgcct tcttcgactt ttcgcgccga
    28141 gtgctcacgc tctccaattc aggactcccg tacccgattc aatgcaccgc cacttcgtgc
    28201 gggcagatcg aattgccggg gctgccgctg ggctcctttc ccggggcgac ctacgacgaa
    28261 gtcgcgatcg acctccacaa gggggatatc ttcgtgttcg ccaccgacgg gatcctcgag
    28321 acactcaacg acgggggggt cgagttcggt gctctccgat tggccgaggt cgtccagagc
    28381 aatcgcgacc agtcggcacg gaccatcgtc gacaggattt tcgacgccgc gaacgggttt
    28441 cgaggcagca ggccgcaggc cgatgacatg accgcggtcg cggtcaagat cacggtgtag
    28501 ctgttggcgc ttgggacccg gtgaggaaga cggtcgcgtc cggatcgtga atagggcccg
    28561 gagcacggcg atgccagcgc ctgcctgaag gttacgtacc ccggtgatcc ggcgcgacct
    28621 gtctcccgtt aattcaagat gcgtgccccg tgatcccggt cgaattgcgg cagaattcga
    28681 ggctcgtttc tccgcgagcg tccacctctg gagtcgcggc cgatcttggc gccgtcccct
    28741 gaactgtccg atcgaccggg cattgaccaa ctttcagacc cgttccggcg tctgagtagc
    28801 tgaaacacgg tcacggccac atccggccga gaggtcaacg tatgagaaag atgatcgtcg
    28861 caggcgggat tctggccgct gttgccttgg tcgcaggagt cgtcgtggcc ctcgccccgt
    28921 ccgccgtcgc gcagcagggg cgttactccc ccttcctgat caccgagggc cctggctcgt
    28981 cgataggtgt cgccgtacgc gacagcgata ccgacgaggc tcggaaagcc gggacaaacg
    29041 gtgtcgtcgt tcaggaagtc cgcgaaggga cgccggctgc ccgcgcgggc gtgcgcacag
    29101 gcgacctgat tgtcgacttc gacggtgagc gcgcgcgaag cgcgagccag ctcaccaggg
    29161 ttgtgcgtga gaccgctccg gggcgggcgg tcaagatgac catcgtccgc gatgcgtccc
    29221 ggcagacgct cgacatcacc cccgaagccc gcggggcgga caacatcatg tcgaacatcg
    29281 tcgccaacgt ccgcacgacg cccggggtgg acggccgcaa cttcgcgttc gactatctga
    29341 cctttgacgg cgcgttctca cgccaacgac tcggtgtgac cgtcacgcag ctcaccgatc
    29401 agctcgcgac gtattttggc gtcaagcagg gcgtgctcgt ttctgaagtg acatcgggct
    29461 ccccggcgga aacggcaggc gtcaaggccg gagacatcat cacgacagta aacagtcgcg
    29521 ccgtgtccac ggcggacgac gtcgttagag aggtacgggc agctgcacca ggttcggccg
    29581 tcgaagttcg tgtgatgcgg gatcggaagc agctgacgtt gacggcgaaa gtgccggagc
    29641 caagccgtcc gccaactgta ttcagcggtc ggacgatcta gtggactgta tcaactggaa
    29701 gagtaccgcg gacaacgatc ttcgaccgcg atttgccggc cgcgacaacg gagcggccgg
    29761 tagtaatccg cgaaggacat accactagct ggacgccgtc cgttcgaacg gcagtatgtc
    29821 ggctggctca gtggagctgc ccgcaggcca gggtgcaaca acggccgaca gctgcgcggc
    29881 gacgagactc acagtcgcct gagtcgtttt gtccgacacg cgctcggaat tgacctcgac
    29941 ggccagcaca ccgatgcacc ggtcggttcc gagcatcggg gcgacgacgg ctcctctcga
    30001 cgtcgcctcg ccacccaccg tctgcaactg tcctgttcgc catgcggccg ccgtcgcgtt
    30061 gactgctccc ttggcgattg gaccaagccg gcctagttgt ccgaggtcgt acccgtaggc
    30121 cgctgccggg aacagttcct cgccaccggc catccagacg acgacccccg gcgcggcgag
    30181 caggttcccg gtccgttcga ggagacgctc gaggtcctct ctcgacttca tgcgtccgat
    30241 tgccgtgcag aggttcgcga ccgccggcag gtcgagcggt gcgggtttca ggtcgagctc
    30301 gaagaccgaa cggtccgcgg cggcaggcgc ggaaggggtg acgctctcgt gtacgggcgt
    30361 ggaagacgac gaaggcccgc gtgtggcgcg cacaagggcc catgcgaaga gcagcgccgt
    30421 caagcccatc gctgccgcaa tcgtcctcag atccgaggct ctggcgaccg ctgcggccgc
    30481 gatctcttcc gcccgcagcg cgcggaactg ctggcgcagc tcgccgcgca gcatgtgtgt
    30541 ctcgctgaag atcaggtcgg cggccatcag atcgaggccg gcccggacgt tttcgcgagc
    30601 gctgccttcg atctccgcga tcgtcgatga cgccgcggcc gcagcccggg cgggggtgct
    30661 gttctcagag agcagtcgag ccagcagcgg aggcgtttcc gacgcgagat ggtgcatcgc
    30721 gttcgacgtg cgctccagcg ccggtccggc ggcgccacca gaagcggcgt aggtcagttc
    30781 ttcctgcgca atctcatcga ggtcggcttc gagccggtcg attcgtcgaa ggttgtcctg
    30841 actgatccgt tcaacctcat cgacgtgccg cacgatagtc caggcccaaa aacccgttcc
    30901 gcagccggct gcgacgaaca cggctgcgag caaggaactg acaatccgcg cgtgcattgg
    30961 agacctcgac agaataccaa aggtcggaga tcggaaatcg gtgctatcga ggagtcgcag
    31021 gaagctgctt tggatgcaga acgacgtaag tgggcaagcc gaccgcgttg aagcggttca
    31081 tgacagatcg tacgggctcc tcgttgaacg actccgcctg gaacttgatc ttcacgtagc
    31141 cgtccagagc ggctttcacc ttttcgtcag cgagagtggt cctgtccatg accagacagt
    31201 tcttgcacca ggtcgcccac aggtcgatca gtacgggctt ctggtcgcgc tgagcaacgg
    31261 ccagcccttc ccgaagcgaa ccgtaccagc cggctttcag tttctcctcg atgctcgacg
    31321 caacctcacg agcgtccacc cagcggtcgg cgaacaaacc gtaggcgaga tagccgtagt
    31381 aagccgccgt ggccaggata aggacgccaa agccctgctt cacccgaacc atccaggtac
    31441 cgggtttggg cagcgccgtg aggcctgctc ccgccagcgg ccacgggacg gccatgccga
    31501 ttccaagcag gaacggcaga gccagtgcgg cagccgttcc cctcgcgtag agatcgctcg
    31561 aaaacaacac gacctgaatc acgacaggcg caacacacgc ccccgccaac agcgctgcga
    31621 ccgcgcccat gccgaaagcc aaagcgaggc tcccacgctg cgagcccgtg ccgagtgtcc
    31681 cggagaagcg agagaagtcg atcgtcagca cgtcgaacat cgccagggcc agcgcaatga
    31741 agacgacggc aatcgccagg ttgaaccaag gtgaagagtt gatcgtgccg aactggccag
    31801 ccgtcaggat cacgatgagt cccaggactc cgtagacggc agccattgca ccgccgtata
    31861 cggcacccag cagaaagccc ctgcctcgcg aacccgaccc ggctcccgcg ccgatgatcg
    31921 caagattgat cgggatcatc ggaagcacgc agggcgtgag attgagcgcc aggcctccga
    31981 gcagcacgag cagcagaatc gccacgggcc cacggccctc gaaaagcccg cggggcgcga
    32041 caccagactc ggcatccctg acgaattgga gaaacgcgtc ggtgccctgg tatccaccgg
    32101 ttgtcgcgac catcgtgaac tggtccagaa gctcggtgct gtccgcggtg gtgcctcggg
    32161 gtgcgatcgg aatgctttcc ctcgcaaccg gggctcgacc gcggccaaac gcgatgcgcc
    32221 cgaaaacctc gggatgccgt gcggtccggg gtgcgcctgc gccaacgatg gtgagtggcc
    32281 agtgcaggtc cgctgtgatc ggcggatagc agagcctctc atcgcaggcc tgatagcgaa
    32341 gcgtcgcggg gacgctgaca gccccgagtg gggcgccagg agcgacgtcg agacgaacgc
    32401 ctatggcgaa ttcctgttcg aacacggcca ggggctcggc ctggcctatt tgttcgaagt
    32461 cggtcgcctc ggggaacacg atctcgccga ccgtgacgcc gtccggcggg tcgatggtca
    32521 gtacggttgg aatcaggctc gggtcgcgag gcgcgtccga ttgcacgtgg aaccggtccg
    32581 gcagacgaac ctgaagtgcc gcacgcacgg aggccccggc agccgcgtcg gattcgacaa
    32641 tcggtgccag ctccgcctgg atgcctcgaa gttgcgcagc ggcgacctgg agtgcgacaa
    32701 ggagcagcgc aacgaccgag accagccccc gcacggctag cccaccggct tgagcggaat
    32761 atacgggctg gccggactct cggcttccat ctggacgttg tggcccttca cggggggcgc
    32821 gtcctggtaa aggaagtccc atttccagcg ctccgaggga ttggacgcct ggagccaatt
    32881 gcgcgcgggc acaggtcgag tgaacgcgat ccgctcggcg tcggcatagg ccgcgggcag
    32941 cttcgccgcg atcgcccggc ggacctcatc gatcgtcgtg tgcggaaggg acgaccccag
    33001 cagccggccc agtgtcacga gaatctgcca gtcctcgcgc gcctctccgg gcggattgat
    33061 cgcccgcgac gcgccctgca ccttcccctg atcgttcgtg tagagtgcgt ccttctcagc
    33121 cgaggcagcg cccggcagca cgatatcggc agccgccgcc aggtccgaca tcacgacacc
    33181 ctgcacgacg agcatcggca gcgtgccctt cctgcgggcc tcgacgatcc atgagacgtc
    33241 gccaagtgag ccatccgggc cgggatccag cacgtacaaa gctttgacgc gtcccgaccc
    33301 gacggcggtg cgcagggcgc cgaggtcggc gtcatggccg ttgcccccgc cgacggcaaa
    33361 gcccacgtcg cgcgcgccat tgacgttggg cgcgtcggtg accggaatgg tgaaccgtgc
    33421 cgaggccggt tgacgcttct cgcttcgcgt ccaggcgacg gtcacgatgc cggcgcctgc
    33481 ctcgccgagc aggccgtcga cgatttcctt gaggacgaag agttcctcgg tggctgcgtg
    33541 cgcggagacg agaaaccgga cggagcccgg gtcggcctga ccggcgatct ggatggcgtc
    33601 ccgcagaccc ggctcgacgt cgtgccacgc cgcgggctcg agcgtcgaac cccgcctccg
    33661 catcggccgg cgcaggcgcg tctcgccttc gacccagctg tactcgaagc gtccgatatc
    33721 gcacatccag tagccgttga catcgaggtt gagccggggt gtcatccgga tcagtcgcga
    33781 gcccttcgcc cactcaggct tggccttcag ccacgcgctg gtgttgcagc ccttcgagca
    33841 gcgcgt
//
LOCUS       DBNGSH010000061         2112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1022657, whole genome shotgun sequence.
ACCESSION   DBNGSH010000061 DBNGSH010000000
VERSION     DBNGSH010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1022657"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="AC16CY_01485"
     CDS             <1..845
                     /locus_tag="AC16CY_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HIR3352474.1"
                     /translation="VVVDPKKAANALRWAVREMEERYKTLAAEGVRNIEQYNRNARYA
                     AAERGAADEEGPRPLPFVVVVIDELADLMMVASNEVEESICRLAQMARAVGIHLILAT
                     QRPSVDVITGLIKANLPSRISFRVSSKTDSRTILDGNGAEQLLGKGDMLFLPPASSRA
                     IRLHGPYISEQESARLASFLRKQGKPVFDETITAEDEKDGTGGQEFEKDELYDEAARI
                     VVSTGQASISYLQRKMRIGFSRAARLVDMMEADGLVSSGTGGKAREVLVDRHYFEEVD
                     AQLR"
     gene            829..>2112
                     /locus_tag="AC16CY_01490"
     CDS             829..>2112
                     /locus_tag="AC16CY_01490"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF023169.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR3352475.1"
                     /translation="MRSFGSLLVTGFVLTSALGVEAQSSSSKAVYTDALAREQVLRRS
                     MDEEKATPEPTALLEQVRTLVGDYERMSRRFPASGYMDNALWQGALLAADSFWTWGAA
                     EDKDAALRLFAAIRSRFPASSLIAQSASHVKRLKQARPQPSDAPVRASLATPPPAILP
                     GVPVALTAIRRDVLSDTVRITIELGREVSFRSERLDGPPRVFIDLDNTRAVESIKDAT
                     IPFPDDVVRQVRVGRQPDTTTRVVMDLAGAGRHSLYALYNPYRVVIDFDRAQAVPAVV
                     PAVAVETRPAPATEAALARSGEARVVAASLPAPEPRPAAAAEPPPPPTPPAVNSSGGF
                     SLSRQLGLGVSRIVIDAGHGGHDPGAKTRSMTEAELVLDVALKLEQLLLKHGGFAVVM
                     TRRDDSFIPLEERTAIANREGADLFLSIHANASTDT"
BASE COUNT          358 a          717 c          704 g          333 t
ORIGIN      
        1 ccgtcgtcgt ggatcccaag aaggccgcca acgcactccg ctgggccgtg cgcgagatgg
       61 aagaacggta caagacgctg gccgcggaag gggtccgcaa catcgagcag tacaaccgga
      121 acgcgcgcta tgccgcggca gaacggggcg cggccgacga agaaggtcct cgccccctgc
      181 cgttcgtcgt cgtcgtgatc gacgaactcg ccgacctgat gatggtggcg agcaacgaag
      241 tcgaggaatc gatctgccgg ctcgcccaga tggcgcgcgc cgtcggcatc cacctgattc
      301 ttgccacgca gcgcccctct gtcgatgtca tcaccggcct catcaaggcg aacctgccgt
      361 cgcgtatctc gttccgcgtg tcgtcgaaga ccgactcgcg gaccattctc gacggcaacg
      421 gcgcggagca gttactgggg aaaggcgaca tgctcttcct cccgccggcg tcctcgcgcg
      481 ccatccgcct tcacggcccg tacatctcgg agcaggagag cgcgcgactg gccagcttct
      541 tgcgaaagca gggcaagccg gtcttcgacg agacgattac ggctgaggat gagaaggacg
      601 gcacgggagg tcaggagttc gaaaaggacg agctctacga cgaggcggcc cgaatcgtgg
      661 tatcaacagg tcaggcatcc atctcctatc tgcagcggaa aatgcggatc ggcttcagcc
      721 gcgcggcgcg tctggtggac atgatggagg cggacggtct cgtctcgtca ggcacgggcg
      781 ggaaggcgcg cgaggtgctc gtggatcgtc actacttcga ggaagtggat gcgcagcttc
      841 ggtagcctgc tcgtcacggg cttcgtgctg acgtccgctc tgggcgtcga ggctcagtcg
      901 tcttcgtcga aggcggtata caccgacgcg ctggcccgcg aacaggtgct gcgccgcagc
      961 atggacgagg aaaaggcgac gcccgagcca acggcgctgc tcgaacaggt gcgcacgctc
     1021 gtcggcgact acgagcggat gtcacgccgg tttccggcga gcgggtacat ggacaacgcg
     1081 ctctggcagg gcgcgctgct cgcagccgat tcgttctgga cgtggggcgc ggccgaagac
     1141 aaggacgccg ccctgcgcct ctttgccgcc attcgctcgc gctttcccgc cagttcgctc
     1201 atcgcccaat ctgcgtcgca cgtcaagcgg ctgaagcagg ctcggcctca gccgtctgat
     1261 gcgccagtcc gggcttcact tgcgacgccg ccgcctgcga tccttcccgg agtgcccgtc
     1321 gccctcacgg ctatccggcg ggacgtgctt tccgacacgg tccgcatcac gatcgaactc
     1381 ggccgggaag tctcgttccg ctccgaacgg ctcgacggtc cgccgcgggt cttcatcgac
     1441 ctcgacaaca cccgcgcggt cgaaagcatc aaggacgcca cgataccgtt cccggacgac
     1501 gtggtgaggc aggtgcgggt cggacgacag cccgacacga cgacgagggt cgtgatggac
     1561 ctcgcgggcg cgggccgtca cagcctctac gcgctctaca acccttaccg cgtcgtcatc
     1621 gacttcgacc gcgcgcaggc ggttcccgcc gtcgttcccg cagtcgcagt cgagaccagg
     1681 cctgccccgg caaccgaggc ggcgctcgca cgcagcggtg aggcgcgtgt cgtggctgcc
     1741 tccctgccgg cgcccgagcc gcggccggcc gccgcggccg agcctccgcc gcctcctacg
     1801 cctcccgcgg tgaacagctc gggcgggttc tcgttgtcac ggcaactcgg tctgggtgtg
     1861 tcgcggatcg tcatcgacgc cggacacggc gggcacgacc cgggagccaa gaccaggtcg
     1921 atgacggagg cggagctcgt cctcgacgtc gcgctcaagc tggaacagtt gctgctgaag
     1981 cacggaggat tcgcggtcgt gatgacgagg cgcgacgact cgttcatccc gctcgaagag
     2041 cggacggcga tcgccaaccg cgagggcgcc gacctcttcc tgtcgattca cgcgaacgcc
     2101 agcaccgaca cg
//
LOCUS       DBNGSH010000062         3444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1362716, whole genome shotgun sequence.
ACCESSION   DBNGSH010000062 DBNGSH010000000
VERSION     DBNGSH010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1362716"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="AC16CY_01495"
     CDS             complement(<1..1513)
                     /locus_tag="AC16CY_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3352476.1"
                     /translation="MRILFSMRHSGALRNYSSTLAELARRGHEIHLSFMQPDKLGDGR
                     MLWELTNDHDGITYGELGKKTPWRFWLGMARVVRCASDYVRYGTPEYKDAHALRKRAA
                     ERVPFFAWVLSLPLVRSQASLALVSRALGLIERAIPTDRWITDLIAAQHPDVVLVTPL
                     VDIGSDQVEYVKSARALGIHSALCVHSWDNLTNKGLIRILPEQIFVWNETQTREAVTM
                     HGARPDQVVATGAPVYDQWFNRRPSTTRDEFCRKVGLPADKPFLLYLCSSQFIAPDES
                     DFIDKWVRAVRSAPDPRVRQLGILIRPHPENLQPWQRFDFSGLHDVVLWPRGGANPVD
                     QGSKNDYYDSMYHAAAAVGVNTSAQIEAGIVGRPVFSVRVPEYAGTQEGTLHFHYLLN
                     ENGGLLHMADSLDAHTADLVKAVVRTEEDDRRLRGFVEGFVRPRGLGQPATPILADAI
                     EELARRPQPAPERLTVGLHLVRLVLHPVGIAMKLVRYVSRLMRKRERQMRPLTV"
     gene            complement(1549..1914)
                     /locus_tag="AC16CY_01500"
     CDS             complement(1549..1914)
                     /locus_tag="AC16CY_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3352477.1"
                     /translation="MQDYHQLDIWQRGMDYTVAVYEFSATLPGDERFNLVAQLRKAAV
                     SVPLNVAEGCGCATNAEFARFLGYAYRSLKEVVTCLELCDRLRIAPESSTAPLIDEGN
                     QIARMTYTLLQRLGGQARD"
     gene            complement(2005..2889)
                     /locus_tag="AC16CY_01505"
     CDS             complement(2005..2889)
                     /locus_tag="AC16CY_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352478.1"
                     /translation="MPSAPEAAGPAGAAPADLWTIEPRQHGVLERTRELWRYRQLWWY
                     FAGEAVESMYRRTKLGWLWLILRVTAPIGLQSLIFGLLLGIPSGGTPYFLFLMCGMTT
                     WIIFERSLLTVTRSLEQHRKLVTKIYFPRMILPLAAVAPGLLYMAVLLVVLVGTTLFH
                     RYRDGVWFITLRPELLVSVAAIVVSLIFTVAVGLWTSVLQARYKDIRQGLRYFLPFWM
                     YFTPILYPMTTIPEDWRWLMTLNPMTAVVEAFKWGTLGTGDLSVSLVSSSLSLVALTM
                     ITGMWFFSREEAASVDQL"
     gene            complement(2873..>3444)
                     /locus_tag="AC16CY_01510"
     CDS             complement(2873..>3444)
                     /locus_tag="AC16CY_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352479.1"
                     /translation="GRLDRDGDPEDVVTEYQDATWDLIGTGVNAGAKGSSRASRYGEI
                     VDVKLISADGREIGSPPRSEDVYVRIRFRTYGAKTRARCAVDLSSKGTLVFRSAQPGP
                     CQVAPDTAYEALVRIPANLLAETNYAIGVLVTLIRGEGEVHALAVKKALTFLVYDETS
                     PGLTAPRQTGMIAPVLEWSLEEKPIAIRA"
BASE COUNT          634 a         1109 c         1128 g          573 t
ORIGIN      
        1 cgacggtcag cggcctcatc tgtcgttcgc gctttcgcat tagccgcgag acgtaccgga
       61 ccagcttcat ggcgatgccg accgggtgca gtacgagacg cacgaggtgg aggcccaccg
      121 tcaaccgctc cggcgccggc tgaggccgcc gggccagctc ctcgatcgcg tcggccagaa
      181 tcggcgttgc cggctggccg agcccgcgcg gccgcacgaa cccttcgacg aagccgcgca
      241 gccggcggtc gtcctcctcg gtgcgcacga cggccttcac gagatcggcg gtgtgcgcgt
      301 cgagcgagtc ggccatgtgg agcaggccgc cgttctcgtt caacaggtag tgaaagtgca
      361 gggtgccttc ctgcgtcccg gcgtactcgg ggactcgcac cgagaacacc ggccgcccca
      421 cgatgcccgc ctcgatctgc gcgctggtat tgacgcccac cgcggccgcc gcgtggtaca
      481 tcgagtcgta gtagtcgttc ttcgagccct gatcgaccgg gttcgcgccg ccccgtggcc
      541 agaggacgac gtcgtgcaga ccggagaagt cgaagcgctg ccacggctgc agattctccg
      601 gatgcggacg aatgagaatc ccgagctggc gaacgcgcgg gtcgggcgcc gagcgcacgg
      661 ccctcaccca cttgtcgatg aagtcggatt cgtcgggcgc gatgaactgc gacgagcaga
      721 ggtagaggag aaacggcttg tccgccggca ggcccacctt gcggcagaac tcgtcgcgag
      781 tcgtgctcgg gcggcggttg aaccactggt cgtagacggg agcgccggtc gcgaccacct
      841 ggtcgggcct cgcgccgtgc atcgtgacgg cctcacgcgt ctgcgtctcg ttccagacga
      901 agatctgctc ggggaggatc cggatgaggc ccttgttcgt caggttgtcc cagctgtgca
      961 cgcagagggc gctgtggatg ccgagcgccc gcgccgactt gacgtattcg acctgatctg
     1021 aaccgatgtc cacgagcggc gtcacgagca cgacgtccgg atgctgcgcc gcgatgagat
     1081 ccgtaatcca ccggtcggtc gggatcgcac gctcgatcag gccaagcgca cgcgacacga
     1141 gcgccaggct cgcctgcgag cgcacgagcg gcagcgagag cacccaggca aagaacggca
     1201 cgcgctcggc ggcgcgcttc cgcagcgcgt gcgcgtcctt gtattcgggc gtgccgtacc
     1261 gcacgtagtc ggaggcgcag cggacgaccc gggccatccc cagccagaac cgccacggcg
     1321 tcttcttgcc gagctcgccg taggtgatgc cgtcgtgatc gttggtcagc tcccacagca
     1381 tccggccgtc gccgagcttg tcgggctgca tgaacgagag gtgaatctca tgcccgcgcc
     1441 gggcgagctc ggcgagcgtc gacgaataat tgcgcagcgc gcccgaatgg cgcatggaga
     1501 acaggattcg catctcggag ttttgagtct tgagtttcga gttttgagtc agtcccgagc
     1561 ctgtccgccg agtcgttgta acaacgtgta agtcattcga gcgatctgat tgccttcgtc
     1621 gatgagaggc gccgtggacg attctggcgc gattcgaaga cggtcacaca actcgaggca
     1681 cgtaacgact tccttcaagg aacgatatgc gtagccgaga aatcgggcga actcggcgtt
     1741 ggtggcacat ccgcagccct cggccacgtt caacggaaca gaaaccgccg ccttgcgcag
     1801 ctgggccacc agattgaatc gttcgtcacc gggcaaagtc gcggagaact catacacggc
     1861 cacggtgtaa tccatgccgc gctgccatat atcgagctga tggtagtcct gcacttggac
     1921 ggccagtctt tagttttgag ttttgagttg tgagtcttga gttatgaatc ttgagttctg
     1981 aactcactac tcataactca caactcacaa ctgatccacc gacgccgctt cctcccggga
     2041 gaagaaccac atccccgtga tcatcgtcag cgcgacgagc gagagcgagc tcgagaccag
     2101 cgacaccgac agatcgcccg tgccgagcgt gccccacttg aacgcctcga cgacggcggt
     2161 catcgggttg agcgtcatga gccagcgcca gtcttcagga atggtggtca tcgggtagag
     2221 gatgggcgtg aagtacatcc agaacggcag gaagtaccgg agcccctgcc ggatgtcctt
     2281 gtagcgcgcc tggagcacgg aggtccacag gccgacggcc accgtgaaga tcaggctgac
     2341 gacaatcgcc gcaaccgaaa ccagcagttc gggacgaagc gtgatgaacc agacgccgtc
     2401 ccgatatcga tggaagagcg tcgtgccgac caggacgacg agcagcacgg ccatgtacag
     2461 caggccgggc gccaccgcgg ccagcgggag gatcatgcga ggaaagtaga tcttggtgac
     2521 gagcttccga tgctgttcga ggctgcgcgt cacggtgagc agcgagcgct cgaagatgat
     2581 ccaggtggtc atgccgcaca tgagaaagag aaagtacggc gtgccgcccg aggggatgcc
     2641 aaggaggagc ccgaagatca gcgattgaag gccgatgggc gccgtcacac ggagtatcag
     2701 ccagagccag ccgagcttcg tccgccgata catcgactcg accgcctcgc cggcgaagta
     2761 ccaccagagc tgacggtagc gccacagctc gcgcgtccgc tcgaggacgc cgtgctgccg
     2821 cggctcgatg gtccagaggt cggccggcgc cgcgccggcg ggcccggcgg cctcaggcgc
     2881 ggatggcaat cggcttctcc tccaggctcc actcgagcac cggtgcaatc attcccgttt
     2941 gccggggggc cgtgagcccg gggctggttt cgtcgtacac gaggaacgtc agcgccttct
     3001 tgaccgcaag cgcgtgcact tcaccttcgc cgcgaatgag cgtcacgagc acgccgatcg
     3061 cgtaattggt ctccgccagc agattcgccg gaatcctgac gagcgcctcg tatgccgtat
     3121 cgggagcaac ctggcaggga cccggctggg cggaacggaa cacgagagtt cccttcgaag
     3181 acaggtccac ggcgcagcgt gcgcgcgtct tcgcgccgta ggtccggaac cggatccgca
     3241 cgtacacgtc ctcggagcgc ggcggcgatc cgatctcgcg gccgtcggcc gagatcagct
     3301 tgacgtccac gatctccccg tacctgctgg cacggctgct acccttcgct ccggcattga
     3361 cccccgtccc gatcaggtcc cacgtggcgt cctggtattc ggtgacgacg tcttccggat
     3421 cgccgtcgcg gtccagccgg ccgt
//
LOCUS       DBNGSH010000063         3268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2042523, whole genome shotgun sequence.
ACCESSION   DBNGSH010000063 DBNGSH010000000
VERSION     DBNGSH010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2042523"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="AC16CY_01515"
     CDS             <1..1660
                     /locus_tag="AC16CY_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3352480.1"
                     /translation="MKAVLHSVLASLVLASALHMPTRAAGLNIETATVADLNAAFASG
                     TLTSEALVSAYLKRIEAYDKKGPAINAIITLNKNALDEARQLDVERRNGKVRGPLHGI
                     PVVLKDNYDTVDMPTTAGSQLLEGHMARQDAFMVKKLREAGAIVLAKVNLSEFAGSGG
                     SVSGATDPAIIKAGSVPNGSSSAGGQTKNPHELLHGPAGSSGGTGAAIAAAFAQFGLG
                     TDTGGSVRNPSSANGIVGLKPTHGLLSRAGIVPLALSFDTGGPMARNVYDVAVALGVM
                     TGVDPADDATKKSEGKFERDYTKYLKTGALKGARLGIARDFMGKDAGTDAVVEAAIAT
                     LKKLGAVVVDPIKYPDYVLQSKGALYNIVTYAEFKAQIADYLKTSEPQVPKTLDEIVA
                     RANDPKTGYRSPEKAVALKYTASVALDLTDPSYIAAQNEALASTKAAVMALFEKYQLD
                     AIVYPTVPRPAPYIKPDGLPAPADSPTNLANESGFPDLIVPAGMTWSGLPVTISFFGR
                     AFSEGQLLGYGYDFEQATKARVLPKYTPALPSDVVGMTVTGKNP"
     gene            complement(2007..3182)
                     /locus_tag="AC16CY_01520"
     CDS             complement(2007..3182)
                     /locus_tag="AC16CY_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HIR3352481.1"
                     /translation="MPWLETAPMEQRERFIDDSRRGLYDMTELCARYAVSRKTGYKWL
                     ARYDAGGRPALLDRSRAPHHCPHKIAEAVAQLLLTARRRHPDWGPEKLLQWLEPRHPG
                     MAWPAISTAGDLLARHGLVKKRRRRRPMQHPGVVPPVTHAPNDLWAADFKGQFRTGDR
                     VYCYPLTVTDQHTRYLLACHGLLSTRGTGVRPVFDRLFREYGLPRAMRTDNGVPFAST
                     SLHGLTPLNVWWLRLGIQHQRILPAHPQQNGAHERMHKTLKRGAIRPPRASLASQQRA
                     FHRFRQEYNHERPHQFLGGLTPGSRYRPSTRPYTGVLPTLEYPDHFLVKRVTNAGTIR
                     FKTRLLYVSTALKQHRIGLDEVDDGIWSVFFCNVLLGRIDERQALVRGQLGVTHVPG"
BASE COUNT          628 a          974 c         1089 g          577 t
ORIGIN      
        1 catgaaggct gtgctccatt cagtgctcgc atcgcttgtg ctcgccagcg cgctgcacat
       61 gcccacgcgc gcagctggcc tgaatatcga gaccgcgaca gtcgccgatt tgaacgcggc
      121 atttgccagc ggaacgttga cgtccgaggc attggtaagc gcgtacctca agcggatcga
      181 ggcgtacgac aagaagggcc cggccatcaa cgcgatcatc acgttgaaca agaacgcgct
      241 cgacgaggcg cggcagctcg atgtcgaacg caggaacggc aaggtgcgcg gaccgctgca
      301 tggtatcccc gtcgtcttga aggataacta cgacaccgtc gacatgccga cgacagcagg
      361 ctcgcagctg ctcgagggcc atatggccag acaagacgcg ttcatggtga agaagctgcg
      421 cgaggcgggc gccattgtgc ttgccaaggt gaacctcagc gagttcgctg gcagtggcgg
      481 aagcgtcagc ggcgcgaccg accccgcgat tatcaaggct ggatcggtcc ccaacggctc
      541 aagctccgcg ggcgggcaaa ccaagaaccc tcacgagctg ctgcacggtc ccgcaggatc
      601 gagtggcgga acaggggcgg ccattgccgc cgcgttcgcg caattcggct tgggcacgga
      661 taccggcgga tctgtccgta acccgtcatc ggccaacgga attgtgggtc tgaagccgac
      721 gcacgggctg ctcagccgcg cgggcatcgt gccgctcgcg ctgtcgttcg acacgggcgg
      781 accgatggca cgcaacgtct acgatgtggc ggtggcattg ggggtcatga cgggcgtcga
      841 tccggccgac gatgcgacga agaagagcga gggaaaattc gagcgcgatt acacaaagta
      901 cctgaaaacg ggcgcgctca aaggtgcgcg cctcgggatc gcgcgggatt ttatggggaa
      961 ggatgccggc actgatgcgg tcgtcgaggc cgccatcgcg acgctgaaga agctcggcgc
     1021 ggtcgtcgtc gacccgatca agtatcccga ctacgtcctc caatcgaagg gcgcccttta
     1081 caacatcgtt acgtacgcgg aattcaaggc acagatcgcc gactatctga aaacctccga
     1141 gccgcaagtc cccaagacgc tcgacgagat tgtcgcgcgc gccaacgatc cgaagacggg
     1201 ctaccgcagt ccggagaaag ccgtcgcact caagtacacg gcgtcagtag cgctcgacct
     1261 gaccgatccc tcttacattg ctgcgcagaa cgaggcgctt gcatcgacga aggctgcggt
     1321 gatggccctc tttgaaaagt accagctaga tgcgatcgtc tatccaacgg tgccgcgtcc
     1381 ggctccgtac atcaagcccg atggcctccc agcgcccgcg gacagtccga cgaacctcgc
     1441 caatgagagc ggttttcccg atctgatcgt ccccgctggc atgacgtggt cgggcttgcc
     1501 ggtgacgatt tctttcttcg gccgggcgtt cagcgaaggg cagttgctcg gctacggcta
     1561 cgatttcgag caggccacga aggcgcgcgt gttgccgaaa tataccccgg ccctgccaag
     1621 cgacgttgtt ggaatgacag tgactgggaa gaacccgtag gggcgtaagc gaccggcgca
     1681 ccaacgggac atcatcagca atgtgtgcaa ccggaaacat aggtaacaga gttaaccggg
     1741 aacatgggta acacttcttg ggttaccgac gtcaagagta agggcctgtg gtagctgtgg
     1801 gaaaccgcgt cgtgtgcggt tttccaagga gcggggggcg cgttctctgc gtccacggct
     1861 ctggcagctt ccacggccgg ttttccgaat ttgtgagggg cgcacggaga aacgaccgcg
     1921 caggcggctc caggaacgac gatcggcagc cggcctgacc tgtactatcc gcctggagtg
     1981 agctacggga accatcagtg accgagttac ccgggcacat gggtcactcc cagttgccct
     2041 cgtacgagtg cctgccgttc gtcgattcgc cccagcaaca cgttgcagaa gaagacggac
     2101 cagatgccat cgtcgacctc gtcgaggccg atgcgatgct gcttcaacgc cgtcgacaca
     2161 tacagcaagc gggttttgaa tcggatggtg cccgcgttgg tgacccgctt caccaggaag
     2221 tggtcggggt actcaagcgt gggcaacacg cccgtgtacg ggcgggtcga ggggcgatag
     2281 cgcgagcctg gtgtgaggcc gccgagaaac tggtgagggc gttcgtggtt gtactcctgg
     2341 cgaaatcggt ggaaggcgcg ctgctggctc gcgaggctgg cgcgcggcgg ccgaatggcg
     2401 cctcgtttca gggtcttgtg catgcgctcg tgggcgccgt tctgctgcgg gtgggccggc
     2461 aggatccgtt gatgctgaat gccgaggcgg agccaccaca cgttaagggg tgtgagccca
     2521 tgcagcgagg tggaggcgaa cgggacgccg ttgtcggtcc gcatcgcccg cggcagcccg
     2581 tactcgcgga acaggcgatc aaagacgggc cgcacgccgg tgcccctcgt cgagagcagg
     2641 ccgtgacagg ccagcaagta gcgcgtgtgc tggtcggtga ctgtgagcgg atagcagtag
     2701 acccgatccc ccgtgcggaa ctggcctttg aagtcggctg cccacaggtc gttgggggcg
     2761 tgagtcactg gggggacgac gccggggtgc tgcatcgggc ggcgtcgacg acgtttcttc
     2821 acgagaccgt gccgtgcgag gagatcgccg gccgtactga tggcgggcca agccatccca
     2881 ggatgccgcg gctcgagcca ctgcagcagt ttttccggtc cccagtccgg gtgccgccgg
     2941 cgcgcggtga gcagcagctg tgccacggcc tctgcaatct tgtgtgggca gtgatgaggg
     3001 gcgcgactgc gatcaagcag tgcgggtcgg ccgccagcgt cgtaccgcgc caaccatttg
     3061 tagccggtct ttcggctgac cgcgtaccga gcgcagagct cagtcatgtc gtagagcccg
     3121 cgtcgggaat catcaatgaa tcgttctcgt tgttccatgg gtgccgtctc cagccagggc
     3181 atcgatcaac ctcacagtcg atccccagcg tagtgggtgt cacccatgtt cccggtttaa
     3241 tgtgttacct atgtacccgg tttgtacc
//
LOCUS       DBNGSH010000064         4751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2212111, whole genome shotgun sequence.
ACCESSION   DBNGSH010000064 DBNGSH010000000
VERSION     DBNGSH010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2212111"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="AC16CY_01525"
     CDS             <1..587
                     /locus_tag="AC16CY_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3352482.1"
                     /translation="AGGPDWLEKDRFVVQGQAAGTATRADMTAMLRTLLVERFGLKYH
                     VENKDVDAYALVLDRADGKLGPKLQKWDGTCNGKQPPPAQPNSTGPRCAAFFRPPGLV
                     MRGVTMTVLANMLSAQITNLGRPVVDKTGLTGEFDFDLETTFAPLAANAGSADTAAPS
                     VFVALREQLGLKLDPTRAGVKVLVVDSAQRPTEN"
     gene            589..2025
                     /locus_tag="AC16CY_01530"
     CDS             589..2025
                     /locus_tag="AC16CY_01530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3352483.1"
                     /translation="MKPPSLKLRRPGLIAAALVTLAAGVLLAQTTAPDTSSGTQLVQW
                     LTVFNGSDRAARQQFYKERWAYSPNQNFYEDLLDQTGGFELLRVEESTPTRTIAIARQ
                     IDGDAVARVTLEVEPEAPHRIVRFTSQGIPRPADLPIVRVTEGELLKTLRAELGRRAA
                     VDRFWGTVIVAKNGTPVFTGAYGLANRERTIPIRLDTRLKNGSMNKMFTATAVMTLVQ
                     SGKVALDAPLGKYLTDYPNTALASKVTIHHLLTHTGGTGDFFGAQFTAHRMDLKTHKD
                     YVALFGKRDLLFEPGSQWAYSNYGFILLGAVIERVSGQSYFDYVRDHVYKPAGMTSTG
                     SEPEHQVLPNTAVGYTKRAGSREWQANTDSLGYRAMAAGGGFTTVEDLLRFATALTGN
                     RLLSAENTTLLTTGKVKSGGGQYAYGFVDTSVNGVRVIGHSGGAPGQSGDLLILPDSG
                     YVVAALSNLDPPAAPRITNYVASRLPAR"
     gene            2134..3459
                     /locus_tag="AC16CY_01535"
     CDS             2134..3459
                     /locus_tag="AC16CY_01535"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3352484.1"
                     /translation="MVITKKSLPRRTFLRGLGTTMALPFLESMVPAFPARAQTAALTP
                     LRFGAVYVPNGAPMNFWMPKSSDGTLEVTPILAPLEPFRDTITVVGNLSRAGGRTATD
                     HAVSSAGWLSGVVAKQTEAEDISLGITIDQVLAKQVGQDSPFPSLEFATEDFSGYVGG
                     CVPGYSCTYMNTISWAGETSPLPMEINPRVAFERMFGRSGDTAGRLSRMKENRSILDS
                     IAEEARALQPRVSAPDRARLEEYLGNVREIERRIERAEARSATDVASVGAPVGVPASF
                     EEHAALMFDLLAVAYQSDLTRVFTYMMAREASQRTYPALGIPETHHDVSHHGNQPDKM
                     ALHAKIDTHYVQLFAAFVEKLRSMPEADGSVLDHSLIVFGCGMSDGQAHSAYPLPLAA
                     VGRAGGRIKGNRFVVAPEWSSVANFWLGVAGVFGSPIERFGESTARLDL"
     gene            3504..>4751
                     /locus_tag="AC16CY_01540"
     CDS             3504..>4751
                     /locus_tag="AC16CY_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3352485.1"
                     /translation="MRTSAVACVVLVVALAATAGAQIPLIDAVKRGDRAAVRTLLQQQ
                     ADANASEPDGTTVLHWAAQEDDPDAVRLLLSAGAKAESANRYGVTPLQLAAVNGNAAI
                     AKMLLEAGADPDAVLPEGETVLMTAARTGRVDLLQVLLDAGAEPSARERWYGEDALIW
                     AVAENHADAAQLLIDRGSDPDARSAKLDFPRRRNGQSVLSLGSWTPLMYAARENALDS
                     ARTLVKARVDLDAVDPDGATALVVAIINANYEFAAFLLDAGADPNVVDNEAGMGPLYA
                     AVDMHRLAVGHGRPNPRLTGTMDAVDVVKLLLAHKADPNARLKAPLLQRHHTGGDASL
                     GAGATPIMRAAKSGDVEMMKVLLASGADPRMTMPNQSTVLMYAAGLGWRDGSPAAPSY
                     DQGSPEEAAAAIELLLGLGLDINA"
BASE COUNT          769 a         1651 c         1637 g          694 t
ORIGIN      
        1 tcgcgggtgg gcccgactgg ctcgaaaagg accgtttcgt cgtccaggga caggccgccg
       61 gtaccgctac gcgcgccgac atgactgcga tgctgcgcac gctgctcgtc gagcgtttcg
      121 gcctgaagta tcacgtcgaa aacaaggacg tggatgccta cgcgctcgtg ctggaccgcg
      181 ccgacggcaa actcgggccg aagctccaga agtgggacgg cacctgcaac ggcaagcagc
      241 cgccgccggc acagccgaat tccaccggcc cgcggtgcgc cgcgttcttc cgcccaccgg
      301 gcctcgtgat gcggggcgtc acgatgacgg tgctcgcgaa catgctgtcg gcgcagatca
      361 cgaaccttgg ccgtcccgtc gtggacaaga caggcttgac cggtgagttc gacttcgacc
      421 tcgaaaccac gttcgcccct ctggcggcca acgccggctc ggccgacacg gcggcgccct
      481 cggtgttcgt ggcgctgcgg gaacagctcg ggctgaagct cgacccgacg cgggccggcg
      541 tcaaggtgct cgtcgtggac agcgcgcagc ggccgacgga gaactgagat gaagccgcct
      601 tcgctgaagc tacggcggcc agggctcatc gccgcggcgc tcgtgacgct cgcggccggc
      661 gtcctgcttg cgcagacgac ggcaccagac acgtcgtcgg gcacccaact cgtccagtgg
      721 ctcacggtgt tcaacggaag cgatcgcgcc gcgcgccagc agttctacaa ggagcgctgg
      781 gcgtattcac cgaaccagaa cttctacgaa gacctcctcg atcagactgg cgggttcgag
      841 ttgctgcggg tcgaggagtc cacgcccacc cgcacgatcg cgatcgcgcg gcagatcgac
      901 ggagacgccg tcgctcgtgt gacgctcgag gtcgagccgg aagctccgca tcgcatcgtg
      961 cggttcacgt cgcaggggat cccgcgtccc gccgatctgc cgattgtgcg ggtcaccgaa
     1021 ggcgaactgc tgaaaacgct gcgtgccgag ctcggccggc gcgccgcggt ggatcggttc
     1081 tggggcaccg tgatcgtcgc gaagaacggc actcccgtct tcacgggcgc gtacgggctc
     1141 gcgaaccgcg agcgcacgat cccgatccgg ctcgacacgc ggctcaagaa cggctcgatg
     1201 aacaagatgt tcacggcgac cgcggttatg accctcgtcc agtcgggcaa ggtcgcgctc
     1261 gacgcgccgc tcggcaagta cctcacggac tatccgaaca cggcgctcgc gtcgaaggtc
     1321 accatccatc acctgctgac gcacaccggc ggcacgggcg acttcttcgg cgcccagttc
     1381 accgcgcacc ggatggacct caagacgcac aaggactacg tcgccctctt cgggaagcgg
     1441 gacctgctct tcgagccggg aagccagtgg gcctacagca actacggctt catcctgctg
     1501 ggcgccgtca tcgagcgcgt cagcgggcag agctacttcg actacgtgcg cgaccacgtc
     1561 tacaagccag ccggcatgac gtccaccggc tcggagcccg aacaccaggt gctgcccaat
     1621 accgccgtcg ggtacacgaa gcgcgcgggt tcacgcgaat ggcaggccaa caccgactcg
     1681 ctcggctacc gcgccatggc ggccggcggc ggcttcacga cggttgaaga cctgctgcgg
     1741 ttcgccaccg cgctcaccgg caaccggctg ctgagcgccg agaacaccac gctcctgacg
     1801 acagggaagg tgaagtcggg cggcggacag tacgcgtacg gattcgttga cacatcggtc
     1861 aacggcgtgc gcgtcatcgg ccacagcggc ggcgcgccgg gccagtcggg cgatctgctg
     1921 atcctccccg actccggcta tgtcgtggcc gcgctgtcaa acctcgatcc gcccgccgcg
     1981 ccgcgcatca cgaactacgt ggccagccgc cttcccgccc gctgacgcgc gggccggcag
     2041 gcgccgtgtc cgccggaccc gcgccgtcgg gtccggctta gcccggccgg tagactcccg
     2101 cgcccgaagc gggcgaaaat acgccgacgc cccatggtca tcaccaagaa gtccctgccc
     2161 cggcggacgt tcctgcgcgg cctcggcacg acgatggcgc tgccgtttct cgagtcgatg
     2221 gtgccggcgt tcccggcccg cgcccagacc gccgcgctga cgcctctgcg cttcggggcc
     2281 gtctacgtcc ccaacggggc gccgatgaac ttctggatgc cgaagtcgtc ggacggcact
     2341 ctcgaggtca cgccgatcct ggcgccgctc gaaccgtttc gcgacaccat caccgtggtc
     2401 ggcaacctgt cgcgggccgg aggccggacg gcgaccgatc acgccgtcag ctccgcgggg
     2461 tggctgagcg gcgtcgtcgc gaagcagacc gaggcggaag acatcagcct cggcatcacg
     2521 atcgaccagg tgctcgcgaa gcaggtgggg caggactcgc cgtttccgtc gctcgagttc
     2581 gcgacggagg acttctcggg ctacgtcggc ggctgcgttc ccggctacag ctgcacctac
     2641 atgaacacga tctcgtgggc gggggagacg tcgccgctgc cgatggagat caacccgcgg
     2701 gtcgcgttcg agcggatgtt cggccgatcg ggagataccg ccgggcggtt gtcacgcatg
     2761 aaggagaacc ggagcatcct cgattcgatc gccgaggagg cgcgcgcgct gcagccgcga
     2821 gtcagcgcgc cggaccgcgc caggctcgag gagtacctcg ggaacgtccg cgagatcgag
     2881 cggcggatcg agcgcgccga ggcgagaagc gcgaccgacg tcgcctcggt cggcgcgccg
     2941 gtcggcgtgc ccgcgtcgtt cgaggagcac gccgcgctga tgttcgatct gctggccgtc
     3001 gcctaccagt ccgatctcac gcgcgtgttc acctacatga tggcgcgcga ggccagccag
     3061 cggacgtatc cggcgctcgg catccccgag acgcaccacg acgtgtcgca tcacggcaac
     3121 cagccggaca agatggcgct gcacgcgaag atcgacacgc actacgtgca actgttcgcc
     3181 gccttcgtcg agaagctcag gagcatgccc gaggcggacg gctccgtgct ggaccactcg
     3241 ctgattgtct tcggctgcgg catgagcgac ggccaggcgc actccgccta tccgctgccg
     3301 ctggccgccg tcggacgtgc gggaggccgc atcaaaggca accggttcgt cgtcgcgccc
     3361 gagtggagct cggtggcgaa cttctggctg ggcgtcgccg gcgtgtttgg aagcccgatc
     3421 gagcgcttcg gcgagagcac ggcgcgcctg gatctctgat gcacagcgcg tggcgagtgc
     3481 agaaccgcga acgggtctga cccttgcgca cctccgcagt cgcgtgcgtc gtgctggtcg
     3541 tggcgttggc cgcgacggcg ggcgcgcaga tccccctcat cgatgccgtc aagcgcggcg
     3601 atcgcgccgc ggttcgcacg cttctccagc agcaggctga cgccaacgcg agcgagccgg
     3661 acggaacgac cgtgctgcac tgggcggcgc aggaagacga tccggacgcc gttcgtctgc
     3721 tcctgtccgc gggggccaaa gcggaatcgg cgaaccgcta cggtgtgacg ccgcttcagt
     3781 tggccgcggt gaacgggaac gccgcgatcg cgaagatgct gctcgaggcc ggcgcggatc
     3841 cggatgccgt gctgccggaa ggcgagacgg tcctcatgac ggcggcgcgc acgggacgcg
     3901 tggacctgct gcaggtcctc ctcgacgccg gcgcggagcc gagcgcacgg gagcgctggt
     3961 acggcgagga cgcgctcatc tgggccgttg ccgagaatca cgcggacgcg gcgcagctgc
     4021 tcatcgatcg cggctcggac cccgacgccc gatcggcgaa gctggacttc ccgaggcgcc
     4081 gcaacgggca gtcggttctg tccctcggct cctggacgcc gctgatgtac gcggcgcgcg
     4141 agaacgcgct cgactcggct cgcacgctcg tgaaggcgcg cgtcgatctc gacgcggtcg
     4201 atcccgacgg cgccacggcg ctcgtcgtcg ccatcatcaa cgccaactac gagtttgccg
     4261 cgtttcttct cgacgcgggc gcagacccga acgtcgtgga caacgaggcc ggcatgggac
     4321 cgctgtacgc ggcggttgac atgcaccgcc tcgccgtcgg gcacggacgg ccgaatccgc
     4381 ggctcacggg caccatggac gcggttgacg tggtgaagct gctgctcgcg cacaaggcgg
     4441 acccgaatgc gcgcctcaag gcgccgctgc tgcagcggca tcacacgggc ggcgacgcgt
     4501 cgctcggcgc cggtgcgact ccgatcatgc gcgcggccaa gtcgggcgac gtcgagatga
     4561 tgaaggtgct cctcgcgtcg ggggccgacc cgaggatgac gatgcccaat cagtccacag
     4621 tgctgatgta cgccgcgggg ctcggctggc gcgacgggag tcccgcggcg ccctcatacg
     4681 atcagggttc gcccgaggaa gcggcggccg cgatcgagct gctgctcggg ctcgggctcg
     4741 acatcaacgc g
//
LOCUS       DBNGSH010000065         3072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_514431, whole genome shotgun sequence.
ACCESSION   DBNGSH010000065 DBNGSH010000000
VERSION     DBNGSH010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_514431"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="AC16CY_01545"
     CDS             <1..360
                     /locus_tag="AC16CY_01545"
                     /inference="COORDINATES: protein motif:HMM:NF025734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HIR3352486.1"
                     /translation="SRTIQAHTGTAGELAVVLDRSCGDCHSNATVWPWYTHVAPVSWA
                     MAYAVTEGRKAVNFSEWADYPPGAQRTLLSASCQDALDGKMPGPYTLVRPDTRLSTRD
                     IETICTAARQAEAGGAP"
     gene            357..839
                     /locus_tag="AC16CY_01550"
     CDS             357..839
                     /locus_tag="AC16CY_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3352487.1"
                     /translation="MTTTTLEEKVDDFLAQKRIAVAGVSRDNGHHPTGNLIYNRLKKS
                     GHDVFAVNPHMQTFEGDRCYPDLQSIPGGVDGVVIVTRPETTQRIVRDCRDAGVRRVW
                     MHQSFGKGTSVSPEAVEYCRQHDISVIGGACPMMYGAGADFGHTCMRWMLRFTGGLPR
                     "
     gene            complement(888..1706)
                     /locus_tag="AC16CY_01555"
     CDS             complement(888..1706)
                     /locus_tag="AC16CY_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352488.1"
                     /translation="MAAPNRTLSHRFTLCAVAALCLVTSAVQAQWITYKVPGVPRTPD
                     GKVDLTAPVQRTPDGKPDLSGTWHTTGGYFGNLARDLKPGEVLMLPWAEAQVKENIAN
                     LHKNDPMVACLPPGVPRVSLGGSRGMPHPFKVVQTPLLVVLLYETSTNQTFRQVYLDG
                     RTFPADPQPTWLGYSIGRWEGDTLIVDTTGFNGRAWIDTSSGHPQTEAGKVTERFTRR
                     TVGRMDIDITIDDPKAYLKPWHAKVPVDLLPDADLIETFCENERDLHRMDRTPK"
     gene            complement(1784..2548)
                     /locus_tag="AC16CY_01560"
     CDS             complement(1784..2548)
                     /locus_tag="AC16CY_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3352489.1"
                     /translation="MIALTHRLDRTLVVRAARDTVFQYFTDTPRWASWWGAGSAIDAR
                     PGGRMVIRHANGVEVSGEVLEVSPPERIVFTYGYANQPQIPPGSSRVTIRLDSHPQGT
                     LLQLTHEFSDVAPRDEHVQGWRFQLSLFANLIADTASRETSVVDRWFAAWGEPDAGVR
                     EREIDMITAPAVTFGDKYSCLDGADDLKAHLAAVQRFMPGVRLERRGAVRYCLWHALA
                     DWAAVGKDGQQRGSGTSVFVLDAKGRITSVTGFWAG"
     gene            complement(2545..2922)
                     /locus_tag="AC16CY_01565"
     CDS             complement(2545..2922)
                     /locus_tag="AC16CY_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3352490.1"
                     /translation="MDTALLSTLASPRRREILRLLWTGDLTAGEVHQAMPDVTFGAVS
                     LQLKVLLEAGLVECQADRQYRVYRVRREALGPVASMLEEMWTDALARLRLKAELEAAR
                     RGPRPRRRRSPRSRRSRTKPEDT"
BASE COUNT          522 a          973 c         1051 g          526 t
ORIGIN      
        1 agccgcacga ttcaggcgca cacggggacc gcgggtgaac tggctgtcgt cctggatcgc
       61 tcgtgcggcg actgtcattc gaacgcgacc gtgtggcctt ggtacacaca cgttgcgccc
      121 gtgtcctggg ccatggcgta cgccgtgacg gaagggcgaa aagcggtcaa cttttcggaa
      181 tgggccgact atccgcccgg ggcgcagcgg acgctcctgt cggcgtcgtg ccaggacgcg
      241 ttggacggca agatgcccgg tccctacacg ctggtgcgtc ccgacacgcg gctttccacc
      301 agggacatcg aaacgatctg tacggctgcg agacaagccg aggcgggagg tgcgccatga
      361 cgaccacgac gctcgaggag aaagtggatg actttctggc gcagaagcga atcgcggttg
      421 cgggcgtgtc gcgcgacaac ggccaccacc cgaccgggaa tctgatctac aaccgcttga
      481 agaagagcgg gcacgatgtg tttgccgtga accctcatat gcagactttc gagggtgatc
      541 gctgttaccc cgacctgcaa tccatcccgg gtggggtcga cggcgtggtg atcgtcacgc
      601 gacctgagac gacccagcgg atcgtgcgtg actgccggga tgccggtgtc cgccgcgtgt
      661 ggatgcatca gtccttcggg aagggcacga gcgtctcgcc ggaggccgtt gagtactgcc
      721 gtcagcacga catcagcgtg attggcggcg cctgcccgat gatgtacggt gccggcgccg
      781 acttcggaca cacatgcatg cggtggatgc tcaggttcac cggagggctg cccaggtagc
      841 gtctacaccc cgggtcgccg gccgcgccag atacggcgcg gccatcctca cttcggcgtc
      901 ctgtccatgc ggtgcagatc ccgctcgttc tcgcagaagg tctcgatcag gtcggcgtcg
      961 ggaagcagat cgacggggac cttcgcatgc cacggcttga gataggcctt gggatcgtcg
     1021 atggtgatgt cgatgtccat gcggccgacg gtgcggcgcg taaaccgttc ggtcactttc
     1081 ccagcctccg tctgcggatg ccctgagctc gtatcgatcc aggcgcgccc gttgaatccg
     1141 gtcgtgtcca cgatcagcgt gtcgccttcc catcgtccaa tcgagtagcc cagccaggtc
     1201 ggctgcgggt cggctggaaa cgtgcgcccg tcgagataca cctgacggaa cgtctggttg
     1261 gtggaagtct cgtacagcag gacgacgagc agcggcgtct gcaccacctt gaagggatgg
     1321 ggcatgccac ggcttccgcc caggctcacg cgagggacgc cgggcggaag acacgcgacc
     1381 atcggatcgt tcttgtgcag gtttgcgatg ttctccttca cctgggcctc ggcccacggc
     1441 aacatgagca cctcgcccgg cttcaggtcg cgcgccaggt tgccgaaata cccacccgtc
     1501 gtgtgccagg ttccggagag gtcgggtttc ccatccggcg ttctttgtac aggcgcggtc
     1561 aggtcgacct tgccgtccgg tgtacgggga accccgggaa ccttgtaggt gatccactgc
     1621 gcctgaacgg cgctcgtgac gagacacagc gcggcaaccg cacacagcgt aaagcgatgg
     1681 gacaacgtcc gattcggtgc agccatgggg cctccgctcg ttgacggact tactggagcg
     1741 tatcagcgaa accggcgcgc cctatattac gcggatcccg atctcatcct gcccagaatc
     1801 cggtcacgga cgtgattcgc cctttggcgt cgagcacgaa gacgctggtc ccgctgccgc
     1861 gctgttgacc gtccttgcca acagcggccc agtcggccag cgcgtgccac aagcagtacc
     1921 ggacggcacc gcgacgttcg aggcgcactc cgggcatgaa gcgctgcacg gcggcgaggt
     1981 gagccttgag gtcgtcggcg ccgtcgagac agctgtactt gtcgccgaac gtgacggccg
     2041 gcgccgtgat catgtcgatc tcgcgctccc ggacgccggc gtccggttcg ccccacgcgg
     2101 caaaccaccg atccaccact gaggtctcgc gactggccgt gtcggcaatc aggttggcaa
     2161 acagcgacaa ctggaacctc cacccctgaa cgtgctcgtc gcgcggggcc acgtcggaga
     2221 actcgtgcgt gagctgaagg agagttccct gcgggtggct gtcgagccga atcgtcacgc
     2281 gggagcttcc cggcgggatc tgcggctggt tggcgtaccc gtaggtgaag acgatgcgct
     2341 cgggtgggct gacctcgagc acttctcccg atacttcgac gccgttcgca tgccggatca
     2401 ccatccgccc gccggggcga gcgtcgatcg ccgagcctgc gccccaccag gacgcccagc
     2461 gcggcgtgtc ggtgaagtac tgaaacacgg tgtcgcgcgc cgcgcggacg acgagcgtgc
     2521 gatcgagacg atgggtgagt gcgatcatgt gtcctccggc ttcgtgcgag agcgtctgga
     2581 gcgtggggac cggcggcgcc gcggccgcgg cccgcgccgg gctgcctcga gctccgcctt
     2641 gagcctgagg cgcgcgagcg cgtcggtcca catctcctcc agcatcgagg ccacggggcc
     2701 gagcgcctcg cggcgtacgc ggtacacgcg gtattggcgg tcggcctggc attcgacgag
     2761 gcccgcctcg agcaacacct tgagctgcag cgacacggcg ccgaacgtca cgtcgggcat
     2821 cgcctggtgg acttcacccg ccgtgaggtc ccccgtccag agcagccgca gaatctcgcg
     2881 gcggcgaggg gaggcgaggg tggagaggag cgcggtgtcc atgccgacgc tggaagttta
     2941 gataaaacta aaataataag tcaagcttca agggtgcgag ggtgcgaggg tgcgagggtg
     3001 cgaggtgcgg gggtgcctgt ctatttgcgg gcgaagcggt agttgcccga cacgtgcttc
     3061 acgaagaggc cg
//
LOCUS       DBNGSH010000066         2774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4419550, whole genome shotgun sequence.
ACCESSION   DBNGSH010000066 DBNGSH010000000
VERSION     DBNGSH010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4419550"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="AC16CY_01570"
     CDS             complement(<1..1151)
                     /locus_tag="AC16CY_01570"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3352491.1"
                     /translation="MIITKKAIPRRTVLRGIGATVALPLLDSMVPAAKALAQTPAAPT
                     PRFGFVYVPHGSIMREWTPTQEGANFEFTPILKPLESFRKHVTVLTNLSNYGENGHSV
                     SSAMWLSGTFPSKGSLLKLETTVDQLIAQRIGRESTFPSMEFATEDHSSHLGSCAGDF
                     LCSYMSTISWASASQPLPMEINPRVVFERMFGGDRATPEERRARLEKNRSILDAVAQS
                     ARDLSAQLGPGDRTRLAEYTDTVREIERRIVQAEQQRKETGVEAPFTPVGIPDNWEEH
                     VNLMFDLMRLAYQGNLTRVASFMMSRELSTLSYPQIGVADGHHPVSHNNNIPEQVAKK
                     IKVDIYHLSLFAQFLDKLQATPDGDGSLLDHSLFLYGSGMTNGNQHDHE"
     gene            complement(1148..>2774)
                     /locus_tag="AC16CY_01575"
     CDS             complement(1148..>2774)
                     /locus_tag="AC16CY_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3352492.1"
                     /translation="ETVNIIRGLELEHDLEVTLDGERIVLARFGGPKDEQANYLQPTL
                     AGDEMEKRFQKRLKVPAGLHEVGVAFLKKSSATTLELLQPFDRERIDPITPVGIPELD
                     RVTIEGPFNAVRTERSPSRQKLFTCSPASPREEAACARTILGGVARRAYRGTVSETEM
                     TRLLGFYEKERGQGGSFDAGIENALTFLLVNPRFLFRAEQDPPGDARDVELASRLSFF
                     LWSSIPDDELLDLAIGGRLREPATLERQVRRMLKDERARALGSNFAGQWLYLRNLRSL
                     QPDADVFPDFDHNLRDAMQRETELLFESVVLDDRSILTLLDADYTFVNDRLARHYGIP
                     GVYGTQFRRVAVTNEARKGLLGHGSVLALTSQNNRTSPVLRGKYVLTNVLGTPPPEAP
                     ANVPPLDEKPARARTMRDRMEEHRRNPACAGCHKLMDPIGLTLENFDGIGRWRTVDNG
                     AAIDASAQLADGAAVEGPAALRQALLRRKDLFAQNVTERLLTYALGQRVEHFDMPYVR
                     AILKESAKTDYHFSSLVLGIVKSPAFQRRGPKS"
BASE COUNT          418 a          907 c          937 g          512 t
ORIGIN      
        1 ttctcgtgat cgtgctgatt gccgttggtc atcccgctgc cgtagaggaa cagcgagtga
       61 tccagcaggc tcccgtcgcc gtcgggggtt gcctgcagct tgtccaggaa ctgcgcgaac
      121 agcgacaagt ggtagatgtc caccttgatc ttcttggcca cctgctccgg gatgttgttg
      181 ttgtgcgaga caggatggtg gccgtccgcg acgccaatct gcgggtagct cagcgtgctc
      241 agctcgcgcg acatcatgaa cgacgccacc cgcgtcaggt tgccctgata cgccaggcgc
      301 atcaggtcga acatcaggtt cacgtgctct tcccagttgt cgggaatgcc gaccggggtg
      361 aagggcgctt cgacgccggt ctccttgcgc tgctgctcgg cctgcacgat gcggcgctcg
      421 atctcgcgga cagtgtccgt gtactcggca aggcgcgtgc gatcgcccgg tccgagctgc
      481 gcgctcagat cgcgcgcgct ctgtgcgact gcgtcgagga tgctgcggtt cttctccagc
      541 cgggcgcggc gctcctcggg cgtggcgcgg tcgcccccga acatccgctc gaagaccacg
      601 cgagggttga tctccatcgg cagcggctgc gaggccgagg cccagctgat cgtgctcatg
      661 tatgagcaca ggaagtcgcc ggcgcagctt ccaaggtggc tcgagtgatc ctcggtcgca
      721 aactccatcg acgggaacgt ggactcgcgc ccgatcctct gcgcgatgag ctggtccacg
      781 gtcgtctcga gcttgaggag gctgcccttg gacggaaacg ttccgctcag ccacatcgcg
      841 gagctgaccg agtgaccgtt ctcgccgtag ttcgagaggt tcgtcaggac cgtgacgtgc
      901 ttgcggaagg attccagcgg cttcaggatc ggcgtgaact cgaagttggc gccctcctgc
      961 gtcggcgtcc actcgcgcat gatggaaccg tgcggcacgt agacgaagcc gaaccgcggc
     1021 gtcggagcgg ccggcgtctg ggccagcgcc tttgccgccg gcaccatgct gtcgagcagg
     1081 ggcagcgcga cggtcgcgcc gatccctcga agcaccgtgc ggcgcgggat cgccttcttc
     1141 gtgatgatca tgacttcggt cctctcctct gaaacgccgg gctcttgacg atccccagca
     1201 cgagggacga gaaatgatag tcggtcttgg ccgattcctt gagaatagcg cggacgtacg
     1261 gcatgtcaaa gtgctcgacc cgctgcccca gggcgtaggt caggagcctt tctgtaacgt
     1321 tctgagcaaa caggtccttc cggcgcagga gagcctgccg cagggctgcc gggccctcga
     1381 ccgcggcgcc gtcggccagc tgggccgagg catcgatcgc ggcgccgttg tctaccgtgc
     1441 gccagcgccc gatcccgtcg aagttctcga gcgtcaggcc gatgggatcc atcagcttgt
     1501 ggcatccggc gcaggcggga ttccgccggt gctcctccat gcggtcgcgc atcgtgcgcg
     1561 ccctggccgg tttttcgtcg agcggcggaa cgttggccgg cgcctccgga ggcggcgtgc
     1621 ccaggacgtt cgtcaggacg tactttccgc gaagcaccgg tgaggtccgg ttgttctgcg
     1681 aggtgagcgc caggacgctg ccgtgcccca gcagcccttt ccgcgcctcg ttggtcaccg
     1741 ccacacgacg gaactgcgtc ccgtacacgc ccgggatgcc gtagtggcgg gcgagtcgat
     1801 cgttcacgaa cgtatagtcc gcgtcgagga gcgtcaggat gctccggtcg tcgaggacga
     1861 cgctctcgaa cagcagctcc gtttcgcgct gcatcgcgtc gcgcaggttg tgatcgaagt
     1921 ccgggaagac gtccgcatcg ggctgcaggc tgcgcaggtt tcgcaggtag agccactgcc
     1981 cggcgaagtt gcttcccagg gcacgcgcgc gttcgtcctt cagcatccgg cggacctgcc
     2041 gctcgagcgt cgccggttcg cgcagccggc ccccgatcgc cagatcgagc agctcgtcgt
     2101 cgggaatgct gctccagagg aagaacgaga gacgcgacgc cagctcgacg tcgcgcgcat
     2161 cgcccggagg atcctgctcg gcgcggaaca ggaatcgcgg gttcacgagc aggaaggtga
     2221 gggcgttctc gatcccggcg tcgaagctgc cgccctggcc gcgctccttc tcgtagaagc
     2281 cgagcagacg ggtcatctcg gtctcgctca ccgtgccgcg ataggcgcgg cgcgcgacgc
     2341 cgccgaggat tgtccgcgcg caggctgcct cctcacgggg cgacgcgggg ctgcacgtga
     2401 agagcttctg cctgctcggc gaccgctccg tgcggaccgc gttgaagggc ccttcgattg
     2461 tgactctgtc gagctctgga atgccgaccg gcgtgatcgg atcgatgcgc tcccgatcga
     2521 agggctgaag cagctccagc gtcgtcgccg agctcttttt caggaacgcg acgccaacct
     2581 cgtgcagccc ggccgggacc ttcagccgct tctggaagcg cttctccatc tcgtcgcctg
     2641 ccagcgtcgg ctgaaggtag ttggcctgct cgtctttcgg gccgccgaac ctggcgagga
     2701 cgatgcgttc gccgtcgagc gtcacctcga gatcgtgctc gagctcgagg ccgcggatga
     2761 tgttgaccgt ctcg
//
LOCUS       DBNGSH010000067         3239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1872671, whole genome shotgun sequence.
ACCESSION   DBNGSH010000067 DBNGSH010000000
VERSION     DBNGSH010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1872671"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="AC16CY_01580"
     CDS             complement(<1..380)
                     /locus_tag="AC16CY_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3352493.1"
                     /translation="MMHAIRQAALNSALVIALSGVALAQTQDGEELYKRSCAQCHDTG
                     ANRAPARDAFRAMPAERVLAAMETGSMITMANNRTAAERRAIAEFLTGRSLNNPLVTA
                     PSASAMCQASTSSRFDAASGPRWSA"
     gene            643..939
                     /locus_tag="AC16CY_01585"
     CDS             643..939
                     /locus_tag="AC16CY_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352494.1"
                     /translation="MSLSAAHESKKTRQVCTCCRRRKARFEYRGVVRADRDHTLCFEC
                     YRSERERRRAQLLAEPRPAMLPSPFDRTLTERQASHRRLMLRFAEAQVARAKNR"
     gene            complement(982..1803)
                     /gene="pheA"
                     /locus_tag="AC16CY_01590"
     CDS             complement(982..1803)
                     /gene="pheA"
                     /locus_tag="AC16CY_01590"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3352495.1"
                     /translation="MRIAYQGEPGAYSEAAAREYAGPQAETLPCKTFDEVFEAVVKKR
                     ATHGVVPLENSIGGTIHRNYDLLLDHEIAITGEVELDVVHCLQALPGTPLADVKTVYS
                     HPQALAQCERYLKDLGATVEAVYDTAGGAKLVAEQKLKGAAALASRRAAEVFGLEVLQ
                     EAVQDFEYNITRFAIIGGVPPADANKTTIVFALPSTPGALFKALSVFALRDINLTKLE
                     SRPIRGRPWEYLFYVEVEARRDDLHCARAFTHLAEFAKWTRVLGTYKGAERRGTT"
     gene            complement(1812..2672)
                     /locus_tag="AC16CY_01595"
     CDS             complement(1812..2672)
                     /locus_tag="AC16CY_01595"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3352496.1"
                     /translation="MFKRQRTGSVICASCGVLVGVNDDTCYNCKRRNPSLWGYAPAFR
                     ALGTDLGFVPFLTGTCVVLYALSLLLSGSNIGMNGIFSFLAPSHVALRLLGESGAVPI
                     FVDGWWWTVLSAGWLHGSALHILFNMMWVRQLAPATAEMYGPGRMVIIYTVAGVSGFA
                     LSSLAAVYLPSLPFLRGSLYTVGASAAIFGLLGALVYYGRRSGSSHVGSQATSYALIM
                     GLFGFIMPGIDNYAHAGGFVGGYLAGWLLDPLKPERIDHLVIAVLCLVASLASIAASA
                     VRGLQILSLE"
     gene            2779..>3239
                     /locus_tag="AC16CY_01600"
     CDS             2779..>3239
                     /locus_tag="AC16CY_01600"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR3352497.1"
                     /translation="MRIIVGITGATGAIYGIRLLERLREAGAETHLVVSRWGARTLVH
                     ETAYTVKQVEALASVAYAPADMGAAISSGSFRTDGMIVAPCSAKTLAAIAHGFGDNLI
                     HRAADVVLKERRTLVLSVREAPLSSIHLENMLTLSRMGAMVIPPMPSFYNHP"
BASE COUNT          554 a         1081 c         1068 g          536 t
ORIGIN      
        1 gcgctccatc tcggcccgct cgctgcgtcg aaacgcgacg acgtggaggc ctggcacatc
       61 gcggacgccg aaggcgccgt gacgagcgga ttgttcagcg acctgcccgt gaggaattcc
      121 gcgatcgctc ggcgctccgc cgccgtgcgg ttgttggcca tcgtgatcat cgagcccgtt
      181 tccatcgccg cgagcacgcg ttcggctggc atcgcgcgga aggcgtcgcg ggcgggcgcg
      241 cggttggcgc cggtgtcgtg gcactgcgcg caactccgct tgtacagctc ttcgccgtcc
      301 tgcgtctggg cgagggccac gccggagagc gcaatcacga gcgccgagtt cagtgcggct
      361 tgcctgatcg catgcatcat gagcgggatt atagtcggtg gccgtcgaac gactttcgac
      421 ggagatgaat ctacacgtac gctaaccaca gaggacacag tggacacaga gaatcacatc
      481 caacatcact ctgtgttctc tacgactgtg gctaacgtcc gtctcagaat gaagcggcaa
      541 cggatgcaga cggagtgtgc atgtcgccgg tgcccaggcg aagaagaaac gtatgacctg
      601 cgctgtcacg acccccacgg atgatcgcgg tggaggctcg acatgtcgct gtccgccgct
      661 catgagtcga agaagacccg ccaggtctgc acgtgctgtc gtcgccgaaa agctcgtttc
      721 gaatatcgcg gcgtcgttcg cgcggaccgg gaccacacgc tgtgcttcga gtgctatcga
      781 tccgagcgtg agcggcggcg cgcgcagctg ctcgccgagc cccggcccgc catgctgcct
      841 tctcccttcg accgcaccct gaccgaacgg caggcgtctc accgtcgcct gatgctccgg
      901 ttcgccgagg cgcaggtggc ccgggcaaag aatcggtgag ccgcgtgact cgtagagcgc
      961 acgcttcagc gtgcgcgtga actacgtcgt tccgcgccgc tcagcgcctt tgtacgtgcc
     1021 gaggacgcgc gtccacttgg cgaactcggc aagatgagtg aacgcgcgcg cgcagtgaag
     1081 gtcgtcgcgg cgcgcctcga cctcgacgta gaacaggtac tcccagggcc gcccccggat
     1141 cgggcggctt tcgagcttcg tgaggttgat gtcgcgcagc gcgaacacgc tgagcgcctt
     1201 gaagagcgcg cccggcgtgc tgggcagcgc gaagacgatc gtcgtcttgt tggcgtcggc
     1261 cggcgggacg ccgccgatga tggcaaagcg ggtgatgttg tactcgaagt cctgtaccgc
     1321 ctcctgcagc acctcgaggc cgaacacttc cgccgcgcgc cgtgacgcca gcgccgccgc
     1381 ccctttcagc ttctgctcgg cgacgagctt ggcgccgccg gccgtgtcgt acaccgcttc
     1441 gacggtcgcg ccgaggtcct tcaggtaccg ctcgcactgg gcgagcgcct gcgggtgcga
     1501 gtagacggtc ttcacgtcgg cgagcggcgt gcccggcagc gcctgcaggc agtgcacgac
     1561 gtcgagctcc acctcgcccg tgatcgcgat ctcgtgatcg agcagcaggt cgtagttgcg
     1621 gtggatcgtc ccgccgatcg agttctcgag cggaacgacg ccgtgtgtcg cccgcttctt
     1681 cacgacggcc tcgaagacct cgtcgaacgt cttgcacggc agcgtctcgg cctgcgggcc
     1741 cgcgtactcg cgcgccgccg cttcgctgta cgcgcccggc tctccctgat acgcgattct
     1801 cacccttccc cctactctag actcagtatc tgcagccctc gtaccgccga ggctgcaatc
     1861 gacgcgagcg acgccaccag gcagagcacc gcgatgacga gatggtcgat gcgttcgggc
     1921 ttcagcgggt cgagcagcca gcccgccaga taacccccga cgaagccgcc ggcgtgcgcg
     1981 tagttgtcga tccccggcat gatgaatccg aacagcccca tgatcagcgc atagctcgtc
     2041 gcctggctgc cgacgtgact gctgccgctc cgccggccgt agtacacgag cgcaccgagc
     2101 aggccgaaga tcgcggccga ggccccgaca gtgtagaggc tcccacggag gaatgggagg
     2161 ctgggcaggt acaccgccgc gagcgagctc aacgcgaacc ccgatacccc cgcgacggtg
     2221 tagatgatca ccatgcggcc gggaccgtac atttcggccg tggcgggcgc gagctgacgc
     2281 acccacatca tgttgaagag gatgtgcagc gcgctgccgt ggagccagcc ggcgctcagg
     2341 acggtccacc accacccgtc gacgaagatg ggcaccgccc cactctcccc cagcagtctc
     2401 agcgccacgt ggctcggcgc gagaaagctg aagatcccgt tcatgccgat gttgctgccc
     2461 gacaagagta gcgatagcgc gtacagcacg acgcacgtgc ccgtgaggaa gggcacgaag
     2521 ccgaggtcgg tgccgagcgc gcggaaagcc ggggcgtagc cccagaggct ggggttccgc
     2581 cgcttgcagt tgtagcaggt gtcgtcgttg acgccgacca gcacgccgca cgaggcacag
     2641 atgacggatc ccgtccgctg gcgcttgaac acgggagtcg atgatagcct gtaccaactt
     2701 ccaactccca acgcccaacg cccaaacaag gcctcgggaa ttgggagtgt gatttgggag
     2761 ttggaggttg ggagttcgat gagaatcatc gtcggcatca ccggcgcgac cggcgcgatt
     2821 tatggcatcc gtctgctgga gcggctccgc gaggcggggg cggaaacgca cctcgtcgtc
     2881 agccggtggg gggcacggac gctggtccac gagacggcgt acaccgtcaa gcaggtcgag
     2941 gcgctcgcct cagtggccta tgctcccgct gatatggggg cggccatctc cagcggatcg
     3001 tttcgcaccg acggcatgat cgtggcgccc tgcagcgcga aaacgcttgc ggcgatcgcg
     3061 cacggcttcg gcgacaacct gattcaccgc gcggccgacg tcgtgctcaa ggagcggcgg
     3121 acgctggtgc tctcggtccg cgaggcaccg ctgagcagca ttcacctcga gaacatgctc
     3181 acgctctcgc gcatgggggc gatggtgatt ccgccgatgc cctcgttcta caaccatcc
//
LOCUS       DBNGSH010000068         3414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_174815, whole genome shotgun sequence.
ACCESSION   DBNGSH010000068 DBNGSH010000000
VERSION     DBNGSH010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_174815"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="AC16CY_01605"
     CDS             <1..780
                     /locus_tag="AC16CY_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352498.1"
                     /translation="ERTAEDVFLRGQKVLLGRGEVVVDFGEFYARSDDLQLASVNGGV
                     GLAVLEQASLTTILVGRVGIFNETELFASTAVHHLNDRAFVGSTDLASSGRTEFGGVG
                     FGVRRTLLKESAGRPDVIATLHAQIPTGDIPFSAGAGVVFVKSIDPVVLFANANYSHS
                     FSQSFSNLTRVDPEDRFDVSLGYAVGLNDTLAFSTAVSGLFTGATTVGTTTLKQADAF
                     SLRFALTSWLARGLYIEPSVSFGLAGPGDSFAFGVTIPYSF"
     gene            898..1383
                     /locus_tag="AC16CY_01610"
     CDS             898..1383
                     /locus_tag="AC16CY_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352499.1"
                     /translation="MSLVERDYVLRMIQLLARAIARILGLKQAGKLEEALEEVRTTAD
                     MIFGPMRSTLDAIDPQSAAGLLASGEKTEAYATLTAEEASIRELMGDATRARSGERRA
                     LSLYLEALILEGRLSGDARAAVDDLRSKVDERTLSQRYQEALASSVRRGVGDGNPEGT
                     A"
     gene            1383..2789
                     /gene="zwf"
                     /locus_tag="AC16CY_01615"
     CDS             1383..2789
                     /gene="zwf"
                     /locus_tag="AC16CY_01615"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR3352500.1"
                     /translation="MVSTPADALVFYGATGDLAYKKIFPALHQMALHGSLPSRVVAVA
                     KSGWTLDQLSARARESVERSGGLERDAFGTLLKALDYIDGDYADTSTFERLAAALGES
                     RHPVHYLAIPPRLFGLVLQQLARSGASRSGRVIVEKPFGHNLDTARALNRVLHEAFEE
                     RDVYRIDHYLGMNAVQNLLFYRFNNAFVEPIWNRQFVENVQITMAERFGVDGRGQFYD
                     ATGAIRDVVQNHLLQLLTNVAMEPPPSLDQELVRDERVKVLKSIATIQPDDVVRGQFR
                     GYRTEPGVNPSSRVETYVAMRLYINSWRWKGVPFYIRAGKEMATTSTEVVVKLRQAPA
                     IFTPEPPPANCFRFQVTPAQTIAITSFVKLPGDALRGEAVNLLVSEQHDPNEVGAYED
                     LLTDAMRGASTRFARQDYVEESWRIVDRILDDATPVFEYAPGSWGPPEADRVAPSGGW
                     AELLPTESDRTVVTQNVP"
     gene            2786..>3414
                     /locus_tag="AC16CY_01620"
     CDS             2786..>3414
                     /locus_tag="AC16CY_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR3352501.1"
                     /translation="MTPSSAAHSYNVRICHWVNVVACAYLLWSGVHIFLDFPELYWGH
                     TGYRGYPAAFRLEDWGLSWDEAGALGDRRWGRNYHFTVAWVFLVNGLVYVGWNLYSKH
                     FRNRMWPDRGERTFAHVRAELREHVHWGARRHAADGNYGTLQKTSYLILIFVYVPLMI
                     LTGIAQSPGFTAAMPWLLDMFGGRQTARTLHTIGTVALVLFVVVHVLEVA"
BASE COUNT          585 a         1083 c         1138 g          608 t
ORIGIN      
        1 gagcggacag cggaggacgt cttcctgcgc ggtcagaagg tcctgctggg ccgcggggaa
       61 gtggtcgtgg acttcgggga gttctatgcc cgcagcgatg acctgcaact ggcgtcggtc
      121 aacggcggcg tcggacttgc ggtcctggag caggccagcc tgactacgat cctcgtgggc
      181 cgcgtcggga tcttcaacga gacggagctc ttcgcgagca ccgcggtcca tcacctgaac
      241 gaccgcgcgt tcgtcggcag caccgacctt gcctcgagcg ggcgaaccga gttcggcggc
      301 gtcggcttcg gcgttcgccg cacactgctg aaggaatcag cgggccgacc cgacgtcatc
      361 gccacgcttc atgcgcagat tccgacgggc gacatcccct tctcggcagg cgccggagtg
      421 gtcttcgtca agagcatcga cccggtggtg ctcttcgcca acgcgaacta cagccacagc
      481 ttcagccaga gcttttccaa cctgacccgc gtcgatcccg aggacagatt cgatgtatcg
      541 ctcggctatg ccgtagggct caacgacacg ctcgcgttca gcacagccgt ctccggcctg
      601 ttcaccggcg cgacgacggt cggcaccacg acgctgaagc aggcagatgc cttcagcctg
      661 cggttcgcgc tgacctcatg gctggccagg gggctgtaca tcgagccgtc ggtgagtttc
      721 ggtctggccg gtccgggcga cagtttcgcc ttcggagtca cgattcccta ctcgttctga
      781 aggccccagg aatcaggaat caggcctcag ggctgcgcga ggcctcagcc ccgagacggc
      841 cctgattcct gaggcctgag ccctggggcc ttcctcagtt ggagatagga tgagcgtgtg
      901 tcgttggtcg agcgcgacta cgtcctgcgg atgatccagc tgctggcgcg agccatcgcc
      961 aggattcttg gactgaagca ggccggcaag cttgaggagg cgctcgagga agtgcgaacg
     1021 acggcggaca tgatcttcgg cccgatgcgg agcacgctcg acgccatcga cccgcagagc
     1081 gcggcagggc tcctggcgag cggtgaaaag acagaagcgt acgcgacgct cacggcggag
     1141 gaggcgtcga tccgcgagct gatgggcgac gccactcggg cgcggagcgg cgagcgccgt
     1201 gccctctcgc tgtacctgga ggcgctgatc ctcgagggaa ggctcagcgg tgacgcacgc
     1261 gcggccgtcg atgacttgcg cagcaaggtt gatgaacgca cgctctctca gcgttatcag
     1321 gaagcgctcg catcgagcgt gcgtcgtggc gtgggtgacg ggaacccgga aggaacggcg
     1381 tgatggtatc cacaccagca gatgcgctcg tgttctacgg cgcgacgggc gatctggcct
     1441 acaagaagat ctttcccgcg ctgcaccaga tggcgcttca cgggagtctg ccgtcgcgcg
     1501 tcgtcgccgt cgccaagtcg ggctggacgc tcgatcagct gagcgcccgg gcgcgtgaaa
     1561 gcgtcgagcg cagcggtggg ctcgagcgcg acgccttcgg cacgctcctg aaggcgctgg
     1621 actacatcga cggcgactac gctgacacgt ccacgttcga gcgactggcg gccgctcttg
     1681 gggaaagccg ccaccccgtg cactacctcg cgattccacc acggctgttt ggcctcgtgc
     1741 tgcagcagct cgctcgaagc ggggcctcga ggagcggccg ggtcatcgtc gagaagccgt
     1801 tcggccataa tctcgacacg gctcgcgctc tcaaccgcgt cctgcacgag gccttcgagg
     1861 agcgcgatgt ctaccgcatc gatcactacc tggggatgaa cgcggttcag aacctgctct
     1921 tttaccgttt caacaatgcc ttcgtggagc cgatctggaa ccggcagttc gtcgagaacg
     1981 tgcagatcac gatggcggaa cgcttcggcg tggacgggcg cggacagttc tacgacgcga
     2041 ccggcgcgat tcgcgacgtc gttcagaatc acctgctgca gctcctgacc aacgtcgcga
     2101 tggagccgcc gccgagcctc gatcaggagc ttgtcagaga cgaacgcgtc aaggtgctca
     2161 agagcatcgc gaccattcaa ccggacgatg tcgtgcgagg ccagttccgg ggctatcgca
     2221 ccgagccggg agtgaacccg tcgtctcgag tggagaccta cgtcgccatg cggctctaca
     2281 tcaattcgtg gcgatggaag ggcgtgccct tctacattcg cgccggaaag gagatggcga
     2341 caacctcaac cgaggtcgtc gtgaagctgc gtcaggcgcc ggcaatcttt acgccggagc
     2401 cgcctcccgc aaactgcttc cgctttcagg tcacaccggc gcagacgatc gccatcacat
     2461 cgttcgtgaa gctgcccgga gacgcgctgc ggggcgaggc agtcaacctc ctggtgagcg
     2521 agcagcacga tccgaacgag gtcggtgcgt acgaagatct cttgaccgat gcgatgaggg
     2581 gtgcctccac acgcttcgct cgccaggact acgtggagga gtcctggcgc atcgtggacc
     2641 gcattctgga cgatgccacg cccgtgttcg agtacgcgcc aggaagctgg ggcccaccgg
     2701 aggcggatcg ggtggctccg agcggagggt gggccgagtt gctgcccacc gagtccgaca
     2761 ggaccgtcgt cacgcagaat gtgccatgac cccgtcgagc gccgctcact cgtacaacgt
     2821 ccgcatctgc cactgggtca acgtcgtcgc ttgtgcgtac ctgctctgga gcggcgtcca
     2881 catcttcctc gattttccgg agctgtactg gggtcacacg ggttatcgtg gttatccggc
     2941 ggcgttcagg ctggaggatt ggggcctctc gtgggacgag gcgggcgcgc tcggcgaccg
     3001 caggtggggg cgcaactacc acttcaccgt tgcctgggtg tttctcgtca acggtctcgt
     3061 gtacgtcggc tggaatctct actcgaagca ttttcgcaac cggatgtggc ccgatcgcgg
     3121 cgagcggacc ttcgcgcacg tgcgcgcgga gctgcgcgag cacgtgcact ggggagcacg
     3181 caggcatgcc gccgacggca actacggcac gctgcagaag acgtcgtatc tcatcctgat
     3241 cttcgtgtac gtgccgctga tgattctgac cggcattgcg cagtcacccg gcttcacggc
     3301 ggcgatgccg tggctgctgg acatgttcgg cggccgccag accgcgcgca cgcttcacac
     3361 gatcggcacc gtggccctgg tgctgttcgt cgtcgttcac gtgctggagg tcgc
//
LOCUS       DBNGSH010000069         3599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3061433, whole genome shotgun sequence.
ACCESSION   DBNGSH010000069 DBNGSH010000000
VERSION     DBNGSH010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3061433"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..224
                     /locus_tag="AC16CY_01625"
                     /pseudo
     CDS             168..224
                     /locus_tag="AC16CY_01625"
                     /inference="COORDINATES: protein motif:HMM:NF028194.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            283..561
                     /locus_tag="AC16CY_01630"
     CDS             283..561
                     /locus_tag="AC16CY_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352502.1"
                     /translation="MYGLISGYNATEQPSGPKNLRAVLVNRLRMQGLIVFDWVARYPE
                     AIAALGAWHAEGRLKMREDVRDGGIDAYPDVLNLLYTGGNFGKLVLRV"
     gene            complement(669..1619)
                     /locus_tag="AC16CY_01635"
     CDS             complement(669..1619)
                     /locus_tag="AC16CY_01635"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR3352503.1"
                     /translation="MRSRIVVKGILAAGLIVGVQLALGQIPFAQQAPPPAAPAADAPQ
                     AARGGGRGAVDPRVQQRTYTFADTNEQMPYALFVSSKVTRDKKSPLIVSLHGLGGDQN
                     TMVRESLRSVELAEQGGYILVAPMGYNSGGWYGIPPGPPRGAGANAAARGRGRGAPRP
                     VIGGTAITDAAKVREASEKDVMTVLAMVRKEFNVDERRIYLMGHSMGGAGTYYLGSKH
                     GKEFAALAPIAPAAMGMTNDRTKVLQAIKDAGVPMLVSMGDADEAVPVANVRMWVDTM
                     KELQMNYEYKEYPGVTHGPIMAASMADIYAFFARHSRPAR"
     gene            1843..2535
                     /locus_tag="AC16CY_01640"
     CDS             1843..2535
                     /locus_tag="AC16CY_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3352504.1"
                     /translation="MAHRERHRSERIGWLRAAVLGANDGIVSTASLVVGVAAAEAARQ
                     DVLVAGVAGLVAGALSMAAGEYVSVSSQADTERADLTRERHELATEPQAEEDELTGIY
                     VRRGLDPDLARTVARQLTAKGALAAHARDELGLTEELAARPLQAALASAATFAVGAGV
                     PILTILAAPATALVEAVSIVSLLCLVALGAVAARIGGAPMLVGAARVTIWGVLAMLAT
                     AAVGRLFGTTVA"
     gene            2685..2933
                     /locus_tag="AC16CY_01645"
     CDS             2685..2933
                     /locus_tag="AC16CY_01645"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3352505.1"
                     /translation="MATTTVSSKGQVVIPRALREKYRLTSGTRLHVAESDEGLVLAPL
                     KHARSSRRQAGWRALRGSAKGTAALKEHLAEHRREAKR"
     gene            2917..3348
                     /locus_tag="AC16CY_01650"
     CDS             2917..3348
                     /locus_tag="AC16CY_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3352506.1"
                     /translation="MKRNGRACLDAFAWLAWLQDEPGAAAVQHWLDETEAARAECVTS
                     VINLGEAYYRLVRVDRREEAESLWRMALRGTLPVSVKDATRRRVRRAAELKSKYAIAY
                     ADAFAVATAVECDATLLTGDPEIEPLEGEQNLKVQWLPRKP"
BASE COUNT          603 a         1157 c         1185 g          654 t
ORIGIN      
        1 cctggcggcg gcgtcatcac gtacgccgtc ggcggaaagc agtacgtcgt cttcgtggcg
       61 ggcacgcgct cccgcgtgtt ccccgtctct gccgcgagcg ccaagatcgt cgtcttcggg
      121 ttgtgaatcc caaccgcctc gttcgtctga agagccgccc gagcggcatg gccacgcgcg
      181 agaacttctc catcgacacc gagcccgttc ctccgctgcg ggacggacag atcgaagtcg
      241 acacgagatt cgtttctctc gatctttacg gccgcattgc ttgtgtacgg actgatttca
      301 ggctacaacg caacggagca gccgtcgggt ccgaagaacc tgcgcgcggt tctcgtcaat
      361 cgtctgcgca tgcaggggct catcgtgttc gactgggtgg cgcgctatcc ggaggcgatc
      421 gccgccctgg gtgcgtggca cgccgaaggc aggttgaaaa tgcgcgagga cgtgcgcgac
      481 ggcggcatcg acgcttatcc cgacgtgctc aacctcctct acaccggcgg caacttcggc
      541 aagctggtgc tgcgggtctg aactggcgcg ccagcgccgc tcgatctgga gactgaccct
      601 cccgcggatg cgtcgcgacg gtctgtgttg cgagggcgcc cctcgtggga gcaccctcgc
      661 ggcacggttc agcgggcggg tctcgagtgc ctggcgaaga acgcgtaaat gtccgccatc
      721 gaggccgcca tgatgggacc gtgcgtcaca ccggggtact ccttgtactc gtaattcatc
      781 tggagttcct tcatggtgtc cacccacatc cgcacgttcg ccacgggaac ggcttcgtcg
      841 gcgtctccca tgctcacgag catcgggacc ccggcatcct tgatggcctg aagcactttg
      901 gtgcggtcgt tcgtcatccc catggccgcc ggcgcaatcg gcgcgagcgc ggcaaattcc
      961 ttcccgtgct tcgagcccag gtaataggtt ccggcgcctc ccatggagtg gcccatcaga
     1021 tagatccggc gctcgtccac gttgaattcc ttacggacca tggcgagcac ggtcatgaca
     1081 tctttctcgc tcgcctcgcg caccttcgcc gcatcagtaa tcgccgttcc tccgatcacg
     1141 ggacgcgggg ccccgcggcc gcgtccgcgc gctgccgcat tcgcaccggc gccgcgcggc
     1201 ggacccggag ggatgccgta ccagccaccg gagttgtagc ccatcggagc gaccaggata
     1261 tagccgccct gttcggccag ctcgacggag cgaaggctct ctcgcaccat ggtgttctga
     1321 tcgccgccga ggccatggag tgaaacgatg agcgggctct tcttgtccct ggtcaccttg
     1381 gaagacacga acagcgcgta cggcatctgc tcgttggtgt ctgcaaatgt atatgtgcgc
     1441 tgctggacgc gcggatcgac tgcccctcga cctccgccgc gagcggcctg tggggcgtca
     1501 gcggccggcg cagccggtgg cggcgcctgt tgcgcaaagg gaatttgccc cagcgcaagc
     1561 tggacgccga cgatcaatcc ggccgcgaga atgcccttga cgacgattct cgatctcatg
     1621 gttcctccgt gctgcaggtg acgacgcgcc gcgagcgact cgaatcgttc gcagtatacg
     1681 cacgttgcga gcggttgggg cggctgattc gagcgatggg gcatattacg aaacgacata
     1741 acggatattc gcgtctttgc gacacgcgtc cttgaagcct tgtcgatttc ttcaggtcag
     1801 cgatcgtcgg cacctacaat cgtcgaatga gcgaggagcg caatggcaca tcgtgaacgg
     1861 catcggagcg aacggatcgg atggctccgc gcggcggtgc tcggcgccaa cgacggcatc
     1921 gtgtcgaccg cgagtctcgt cgtcggcgtg gcggcggcgg aggcggcccg ccaagacgtc
     1981 ctggtcgcgg gtgtcgcggg actcgtcgcg ggcgcgctgt caatggccgc aggcgaatac
     2041 gtgtcggtga gttcgcaagc ggacaccgaa agggcggatc tcactcggga gcgccacgag
     2101 ctggcgaccg aaccgcaggc cgaggaggac gagctgaccg ggatctatgt ccgccgcggg
     2161 ctggacccgg acctggctcg aaccgtggcg cgtcaactga cggcgaaggg cgcgctggcc
     2221 gcccacgcgc gcgacgaact gggcctgacc gaagaacttg cggcgcgccc tctgcaggcg
     2281 gcgctcgcat cggcggcaac gttcgccgtt ggcgccggag tgcccattct gacgatcctc
     2341 gccgcaccgg cgaccgcact ggtggaggcg gtctcgatcg tctcgctgtt gtgcctggtc
     2401 gcactcggcg ccgtcgccgc gcgaatcggt ggagcaccca tgctggtcgg tgcggcgagg
     2461 gtcaccatct ggggagtgtt ggcaatgttg gcgacggcgg ccgttggacg gctgttcggc
     2521 acgacggtcg cgtgaacagg ccctacgcat ccagtgtcat tgaaacggtg gagtggacaa
     2581 gactctcacg gccatgctga cctcaccagc agccgctcat cgtcgtatca gcctgcgccc
     2641 gggccgtcgg ccatcgtctt accagcagtg gtaagatatg accgatggcc acgactaccg
     2701 tttcgtccaa ggggcaggtc gtgattccgc gcgcactgcg ggagaagtac cggctgacgt
     2761 caggaacccg cttacatgtt gctgaatccg acgagggcct cgtgctggcg cccctcaagc
     2821 acgcacgatc atcacggcgt caagccggat ggcgcgcgtt gcgcggatcc gccaagggca
     2881 ctgccgcatt gaaggagcat ctcgccgagc acagacgtga agcgaaacgg tagagcctgc
     2941 ctcgatgcgt tcgcctggct cgcctggctt caggacgagc caggtgccgc ggcggttcag
     3001 cattggcttg atgagaccga ggccgcacgc gccgagtgcg tgacctcggt catcaacctc
     3061 ggcgaagcgt actatcgcct ggtccgtgtc gatcgacggg aggaggcaga gtcgctctgg
     3121 cgcatggcat tgcggggaac tcttcccgtt tccgtcaagg atgcgacgcg ccgacgagtg
     3181 cgccgtgcgg ccgagctcaa atcgaagtac gcgattgcct acgccgatgc cttcgccgtc
     3241 gcgacggccg tcgagtgtga cgcgaccctt ttgaccggcg accccgagat cgagcccctt
     3301 gaaggtgagc agaacctcaa ggtgcaatgg ctgcctcgaa agccctgatg gcgcgactct
     3361 gacgatgttc gcgatcagcc ggtcgcggaa attgtccctc acacccagcc agggcatcac
     3421 cacaactagc gtttgtcgag gccgttctcc gcgaggaact tcgagaggag atccgccacg
     3481 gcaacgttgt tcatgtcggc gaagggaatg tgcgtgttgc ccttgaggcc ggcgtcgggc
     3541 agcagcagca cctgggcctt gccgccgtac ttgttgacct tctcggcgaa cagcttcga
//
LOCUS       DBNGSH010000070         6007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4080545, whole genome shotgun sequence.
ACCESSION   DBNGSH010000070 DBNGSH010000000
VERSION     DBNGSH010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4080545"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16CY_01655"
     CDS             complement(<1..230)
                     /locus_tag="AC16CY_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352507.1"
                     /translation="MSDLGYALRTLTRHPGPTTVVVLTLAVAIAATAVIYSAIDTVWH
                     FIPIPKRGGLVYAASTDTRVIQSDVGGRSVVFR"
     gene            complement(396..860)
                     /locus_tag="AC16CY_01660"
     CDS             complement(396..860)
                     /locus_tag="AC16CY_01660"
                     /inference="COORDINATES: protein motif:HMM:NF020496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR3352508.1"
                     /translation="MSTGHTFTAKILPGRGGGAYAEVPFDVEQAFGEKQPQVKASFDG
                     ESFITRLVRMGTPCHIVGIPKDLRVKTGKTIGDRVTITVVPDNAPRVITVPADLKRAF
                     KSEKAAQLFFDGLSYTHRKEYVRWIEDAKKEETRAARVAKTIEMLKAGKRGR"
     gene            complement(913..2622)
                     /locus_tag="AC16CY_01665"
     CDS             complement(913..2622)
                     /locus_tag="AC16CY_01665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3352509.1"
                     /translation="MTTRLHVLLLALLVLASPLVRAQGPPADITLDAAGRAAVIDGAL
                     KVLNDSYVFADVAKKMEQAIRARQQRKEYDAITSGRQFAQTLTNHLREVSKDKHLGVD
                     YSPQALPPQPPPPSPAAPPPAGPQTAIERQAVVMGRQNFGFVKVERLAGNIGYVDFRA
                     FMPPAIAGETAAAAMTFLASCDAVIFDMRQNGGGDPAMVALVTSYLFGPQPVHLNDFY
                     FRPTNETRPSYTLAYVPGKRLTNKDAYVLTSSRTFSGAEEFTYNLKHLKRATIVGETT
                     GGGAHLTMGRRINEHFSIGVPSGRPINAVTKTDWEGVGVEPDVKVPANDALKTAHMLA
                     LEKLEKTLPADATGLRNEAAATLSKLREEIAAPAPASAVSSLAPVPASKAGDDFESGT
                     LANWTIDVAGEGGWFVYTNGKAAPRPALSDPNVPFDVPNPPQGKFGAVTDMSGPGRRI
                     MYRDVTLDGRYRVHLTVFYVDTAGFVATDPSSQTIGDDQQYRIDLVSPSAPVTSLAKD
                     HLLATIFQGKPGDPLSRAPTPLTFDLSPWAGQTVRLRLTSADNVGPLRAGVDDIRFER
                     IGQ"
     gene            complement(2627..3484)
                     /locus_tag="AC16CY_01670"
     CDS             complement(2627..3484)
                     /locus_tag="AC16CY_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3352510.1"
                     /translation="MPRQLTAGRFFGATLQHRRLSGLTLVESVHPAGSTLPRHSHERA
                     YFCINESGTYVERYGRRQRTCSEGWLVYHPPGEVHSERHGPTGVRALNVELEGEWLGQ
                     VLHAVGPLDQPAEFRSGPVGALAERLVDESRRSDLDSALAVESLTWEILTACANPCRV
                     ATDTAAPRWLRDARDLMDICVHEPLSLRRLAREAGVHPVHFAATFRRFHRMSVGEFVR
                     RRRLEYACEKLDDPTLTMAEIAHDAGYADQSHFTRTFKRYIGVTPGQYRRARSPFSRS
                     RAADRPRLD"
     gene            complement(3560..>6007)
                     /locus_tag="AC16CY_01675"
     CDS             complement(3560..>6007)
                     /locus_tag="AC16CY_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352511.1"
                     /translation="GIGVNTAIFSTFERLLLRRLPVAAPDQIVNITSPGPRPGSSSSS
                     DAGGSDAIFSYPLFRDLERVGPDSIRMAAHRDFGANLAYRGQTSDAEGLLVSGQYFPA
                     LGLTPALGRLLGPEDDRVQGAHPVVVLSHAYWSTRFGADPAVIDDTLVVNGEPMTIVG
                     VSPEGFSGNTTMDRPAIFVPLMMAERAYRQPRWNGMTARNNHWLYLFARIGPGVSREQ
                     AEGLMNVPFAALIRDVEFPALRSGMGDRQREQFQQRRIVLQDGARGRNGGRTEVQTVL
                     LLMFAITVFVLVIACANVANLLLARVADRSQEMAVRLSIGASAGRLVRLLLVEASVLG
                     LAGALGALGVARLTLDGLLAIMPAEDVPMLSFGINAPVLLFTLALGLGTSILFGLFPA
                     LHGVRSAALGGLHGQTGRASGSRSANRFRGALATSQIALATALLALAGLFVVSLLNLA
                     RVELGIRREGLVTFRLSPYLNGYSPERAQALFDQVEERLRALPEVMAVTASTVPILSS
                     SNWNNNVTVEGFDAGPDADTRVSVARVGTHYFRTLGITLLAGREFERTDTESSPRVAV
                     VNEAFGRKFNLGPNVIGKRLAMGAGGNRPLNIEIVGLVRDARYSDVRDPPPPQLIMPY
                     RQPDTARGETGVGTLTYYVRTTVNARAFIDEVPRVIGALDRNLPIVALRTMDDQVWDN
                     TTRDRVLGTLSSSFAALATVLAAIGLYAVLAYGVARRLREIGIRVALGAQPRDVRRLV
                     LSQVGRISLIGGAIGVVLALALGRLGRAMLFGVGASDLTVIGGAALLAIVVSFAAGIL
                     PARRATSVNPIEALRVD"
BASE COUNT         1048 a         1970 c         2012 g          977 t
ORIGIN      
        1 cgaaagacga cgctgcgccc gccgacgtcc gactggatca ctcgagtgtc ggtcgatgct
       61 gcgtacacga ggccgccccg cttggggatg ggaatgaagt gccagaccgt gtcgatcgcg
      121 ctatagatga ccgccgttgc ggcaattgcc actgccaggg tcagcaccac gaccgtcgtc
      181 ggcccggggt gccgcgtcag cgtccggagg gcgtaaccga ggtcggacat agcccgaagc
      241 tgcagtgaca aagcccgtgc cacgtcgtga cgacgggcca actccgcttg ttcgagcaga
      301 ttggcggtgg cgtccggcca cggcctggcg cagtgtccgc ccgtgggacg atctcgttcg
      361 tccccgcaca gggaggaact ggagagtggg gcgtttcagc gcccgcgctt gcccgccttc
      421 agcatctcga tcgtcttggc gacgcgtgcg gcgcgagtct cttccttctt cgcgtcctca
      481 atccaccgga cgtattcctt gcggtgtgtg tagctcagcc catcgaagaa taactgggcc
      541 gccttttcgc tcttgaatgc acgcttcaga tcggccggca ccgttatgac gcggggcgcg
      601 ttgtcgggaa ccacggtgat cgtgacgcgg tcgccgatgg tcttccccgt cttgacgcga
      661 agatccttcg ggatgccgac gatgtgacac ggtgtgccca tgcggaccag gcgcgtgatg
      721 aaagactcgc cgtcgaagga cgccttcacc tgcggttgct tttcaccgaa cgcctgctcg
      781 acgtcgaatg gaacctcggc gtaggcgccg ccgccgcggc ccggcaggat cttggcggta
      841 aacgtatgcc ccgtgctcat ggccacgaga ctctaccacc tgcgactctc tgtgccgagg
      901 ccccgcagag ttctattggc cgatgcgctc gaaccggatg tcgtccacgc cggcgcgcaa
      961 gggacctaca ttgtcggccg aagtcaaccg cagacgcacg gtctgaccgg cccagggcga
     1021 cagatcgaag gtcagcggag tcggcgcccg cgacaacgga tcgcccggct ttccttgaaa
     1081 gatcgtcgcc agcagatgat ccttcgccag cgacgtcacc ggagcagacg gcgagacgag
     1141 atcgatccgg tactgctggt cgtctccgat cgtctggctg gaagggtccg ttgcgacgaa
     1201 cccggcggtg tccacgtaga agaccgtgag atgcacgcgg taccggccgt cgagcgtgac
     1261 gtcccgatac atgatgcgtc tgcccgggcc gctcatgtcg gtcaccgcgc cgaacttgcc
     1321 ctgtggcggg ttcgggacgt cgaacggcac gttcggatcg gagagcgcgg gccggggcgc
     1381 tgccttgccg ttcgtgtaga cgaaccaccc tccctcgccg gcgacgtcta tcgtccaatt
     1441 agccagcgtt ccagactcga agtcgtcgcc cgccttcgac gccggcaccg gagccagact
     1501 cgagacggca ctggccggcg caggtgcggc aatctcctcc cggagcttac tgagtgttgc
     1561 agcggcctcg ttcctgagcc cggtcgcgtc ggctggcagc gtcttctcca gcttctcgag
     1621 cgccagcatg tgtgccgtct tgagagcgtc gttggccggg actttcacgt cgggctcgac
     1681 tccgacgcct tcccaatccg tcttcgtgac ggcgttgatc ggccggccgc tcggtacgcc
     1741 aatcgagaag tgttcgttga tgcggcggcc catcgtgagg tgggcgccgc cacccgtggt
     1801 ctcgccgaca atggtcgccc gcttcaggtg cttgaggttg taagtgaact cctcggcgcc
     1861 cgagaacgtg cgcgagctgg tgagcacgta cgcgtctttg ttggtgagcc gcttgccggg
     1921 aacgtaggca agcgtgtacg acgggcgcgt ttcgttggtc ggccgaaaat agaaatcgtt
     1981 caagtggacc ggctgcggcc cgaacaggta actcgtgacg agggccacca tcgcgggatc
     2041 accgccgccg ttctgccgca tgtcgaagat gacggcgtcg cagctggcca gaaaagtcat
     2101 ggcagccgcg gccgtctcgc cggcgatcgc cggcggcatg aaggcgcgaa aatcgacata
     2161 gccgatgttt cccgccagcc gctcgacctt gacgaagccg aagttctgcc gccccatcac
     2221 aaccgcttgc cgctcgatcg cggtctgggg accagcaggc ggaggagcag cgggtgaggg
     2281 cggaggcggc tgaggcggta gagcctgcgg actgtagtcc acgccgaggt gtttatcctt
     2341 gctgacctca cgcaagtggt tggtgagtgt ctgcgcgaac tggcggccgc tcgtgatcgc
     2401 gtcgtattcc ttgcgctgct gacgcgcgcg aatcgcctgc tccatcttct tggccacgtc
     2461 cgcgaagacg tagctgtcgt tcaacacctt gagcgcgcca tcgatcaccg cggcacggcc
     2521 cgcggcgtcg agggtgatgt cggccggtgg gccttgcgca cgcaccagcg gcgacgcgag
     2581 cacgaggaga gccaacaaca gaacgtggag cctggtagtc atgatgtcag tctagcctcg
     2641 ggcgatcggc cgctcttgaa cgtgagaaag gtgaacgcgc gcgccggtac tggcccggcg
     2701 tcaccccgat gtagcgcttg aaggtgcgtg tgaaatggct ctgatcggcg taaccggcgt
     2761 catgggcgat ctcggccatc gtcaaggtcg gatcgtccag tttctcgcac gcatactcga
     2821 gccggcgccg ccggacgaat tctccgaccg acatgcgatg gaaccggcga aacgttgcgg
     2881 cgaaatggac gggatggacg cccgcctcac gagccagcct ccgcagcgac agcggctcgt
     2941 gcacgcagat gtccatcagg tcgcgggcgt cgcgaagcca gcggggagcg gcggtgtcgg
     3001 tggccaccct gcagggattc gcgcatgccg tcaggatctc ccacgtgagg ctctcgacgg
     3061 ccagcgccga gtcgaggtcc gaacgtcgcg attcgtcgac gagcctctcg gccagcgccc
     3121 cgacgggacc actgcgaaac tcggctggct gatcgagagg accgaccgcg tggagcacct
     3181 ggccgagcca ctcgccctcg agctcgacgt tcagcgcccg cacgcccgtc gggccgtgcc
     3241 tctcgctgtg aacctcgcca ggagggtggt acacgagcca tccctccgag cacgtgcgct
     3301 ggcgacggcc gtacctctcg acgtacgtcc cgctctcgtt gatacagaaa taggcccgct
     3361 cgtgagagtg tcgaggcagc gtcgacccgg cagggtggac ggattcgacc agtgtcaggc
     3421 cggacagccg acggtgctga agcgtcgcgc cgaagaatcg ccccgcggtg agctgacgcg
     3481 gcatagcggt tgaaccatca gacgccgtcg gccgaaaaag ggtccccgcc gtcgctaagg
     3541 ctacggcgag gcaagctgtc taatctacgc gcagcgcctc gatcgggttc accgaggtcg
     3601 cgcgccgagc cggaaggatc ccggcggcga acgacacgac gatcgcgagg agcgcggcgc
     3661 ccccgatcac cgtcagatca gatgctccga ctccgaacag catcgcccgg ccgagacggc
     3721 cgagcgcgag cgccaggacg acgccgatcg cgccgccgat caggctgatc cgcccgactt
     3781 gcgacagcac cagccggcgt acgtcacgcg gctgcgcccc aagcgcgacg cgaatgccga
     3841 tctcccgcag gcgtcgcgcg acgccgtagg cgagcacggc ataaagcccg atagccgcga
     3901 gcaccgtcgc cagcgctgca aacgacgatg acagagtgcc gagcacccgg tcgcgggtcg
     3961 tgttgtccca gacctggtcg tccatcgtac gcagcgcgac gatcggcaga ttgcgatcga
     4021 gtgccccgat gacacgtgga acttcgtcga tgaacgctcg ggcgttcacc gtcgtccgga
     4081 cgtagtaggt gagcgttccc acgcccgttt cgccccttgc tgtgtccggc tgccggtagg
     4141 gcatgatgag ctgcgggggc ggcgggtccc tcacgtcgct gtaccttgcg tcgcgcacca
     4201 gcccgacgat ctcgatattc agcggccggt tgccgcccgc gcccatggcg aggcgctttc
     4261 caatgacgtt cgggcccaga ttgaacttgc gcccgaaggc ttcgttgacg acggcaacgc
     4321 gtggcgagct ctctgtgtcc gtgcgttcga actcgcgacc ggcaagcaaa gtaatcccca
     4381 gtgtccggaa gtagtgggta cccacgcgtg cgaccgacac tcgagtgtcg gcgtccggcc
     4441 ccgcatcgaa tccctcgacc gtcacgttgt tgttccagtt gctgctcgag aggatcggca
     4501 cagtcgacgc ggtgacggcc atcacttcgg gtaaggctcg gagccgctct tccacctgat
     4561 cgaacagtgc ctgggcccgt tccggcgagt agccgttgag gtacggcgac agcctgaacg
     4621 tcacgagccc ctcgcgccgg atgcccaatt ccacgcgggc caggttcagg aggctcacga
     4681 caaagagtcc agcgagcgcg agaagcgcgg tcgcgagcgc gatctgcgac gtggccagtg
     4741 ctccacgaaa gcgatttgcg gatcgcgaac cggaggcgcg gcccgtctgc ccgtgcaggc
     4801 cgccgagcgc ggcggagcgt acaccgtgga gagcggggaa gagaccaaag aggatgctcg
     4861 tacccaaccc gagcgccagc gtaaacagca gcaccggcgc gttgatcccg aagctcaaca
     4921 tcggcacgtc ttcggcaggc atgatcgcca gcaggccgtc cagcgtcagg cgtgcgacgc
     4981 ccagtgcgcc aagggcgccg gccagtccga gtacggaggc ctcgacgagg agcaagcgca
     5041 ccaacctccc ggcggaggca ccgatcgaca ggcgtacggc catctcctgg gatcgatccg
     5101 cgaccctcgc cagcaggagg ttggcgacgt tcgcgcaggc gatgacgagt acgaacacgg
     5161 tgatcgcgaa catcaggaga aggacggtct gcacctcggt gcgcccgccg ttacgcccgc
     5221 gtgcaccgtc ctgcagaacg atccgccgtt gctggaactg ctcccgctgg cggtcgccca
     5281 tcccgctccg cagcgccgga aactcgacgt cgcggatcag cgcggcgaac ggcacgttca
     5341 tgagcccctc ggcctgctcg cgcgagactc cgggtccgat gcgggcaaag aggtagagcc
     5401 aatggttgtt gcgagccgtc atgccattcc atcgaggctg gcgataggca cgctcggcca
     5461 tcatcagcgg cacaaagatg gcggggcggt ccatcgtcgt gttgccggag aatccttccg
     5521 gcgacacgcc gacaatcgtc atcggctcgc cgttgacgac gagcgtgtcg tcgatcaccg
     5581 ccggatcggc gccgaagcgg gtgctccagt acgcgtggct gagcacgacg acgggatgcg
     5641 caccctggac gcggtcgtcc tcgggtccca gcagtcgacc gagagccgga gtgagaccaa
     5701 gggcagggaa gtactgcccc gagacgagca gaccctccgc gtccgaggtc tggccacgat
     5761 aggcgaggtt ggcgccgaaa tcccgatgtg ccgccatccg aatggagtcg ggcccgactc
     5821 gctcgagatc acggaacagg gggtagctga agatcgcatc ggatccgccc gcgtcgctgg
     5881 acgagctcga tccaggccgc ggccccgggg aggtgatgtt gacgatctga tccggcgcgg
     5941 ccacgggaag acgccgcagg agcagccgct cgaaggtcga gaagatggcc gtgttgacgc
     6001 cgatgcc
//
LOCUS       DBNGSH010000071         2322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1702946, whole genome shotgun sequence.
ACCESSION   DBNGSH010000071 DBNGSH010000000
VERSION     DBNGSH010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1702946"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="AC16CY_01680"
     CDS             complement(<1..1266)
                     /locus_tag="AC16CY_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352512.1"
                     /translation="MRLTSGALCVSIAMVGYPIAAQAQSAGKVIERHIEAIGGRKAVE
                     KIVSTDVSGTIRSGGGRSGVFIQRTSRPDRYFMSMSWADSRWRAGFNGRSAWQEDGVE
                     GLRTLYGQSVSRVRAEATYANTRLLDSETLNQVSVIRRDQLRGRPVIVVVAVSPDAMT
                     RTLFFDASSYLLVKDEQQTDAGLEERFFDDYRPVDQVMEPHRVEWHRNGETFLIAVER
                     VTHNVPLGEQAFDVPAVPAERPLDIDALLSAVIRSEQRAGPLRAGYAYTMTSSSGKVD
                     EQGRATLSQGASYETFHIGGQPVSKLVRKRGGEALSEAERRREDERVNKIVREYEQQR
                     LPEKAVPRRQGEPPQGSLVILIPLLDAHVLPAYLRMSAFRNIRRERVRDRPAFVVEFE
                     PRPGVVPNSDFERQTGATAGTLWIDEASEH"
     gene            1310..>2322
                     /gene="moaA"
                     /locus_tag="AC16CY_01685"
     CDS             1310..>2322
                     /gene="moaA"
                     /locus_tag="AC16CY_01685"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR3352513.1"
                     /translation="MNGVLTDSHQRPLRNLRLSVTDRCNLRCSYCMPEPEYVWLPRED
                     ILHFEELEMLVDVFTGLGVDKVRLTGGEPLLRRGLPDLVRRLAARPAIRDLAMTTNGV
                     LLAAQAGALRDAGLHRLTVSLDTLRPDRFRDLTRYDELGRVFDGIATAAPLFPGLKLD
                     TVVIRGVNEDEMVDLVEFARGYGAEIRFIEYMDVGGATHWSGSRVVSRREMLDLLGAH
                     YGEITPVVEASSAPADRYRLADGTVFGIISSTTEPFCDSCDRSRLTADGLWYLCLYAA
                     HGTDLRRPLRAGVSTDHLAKLIRATWEVRSDRGAEERLAARDRSPLIPVDRLRRDPHL
                     EMHT"
BASE COUNT          375 a          818 c          731 g          398 t
ORIGIN      
        1 gtgctccgag gcttcgtcga tccacaacgt gcccgccgtc gcgccggtct ggcgctcgaa
       61 atcactattc ggcacgacgc caggtctggg ctcgaactcc acgacgaagg ccggacgatc
      121 tcgaacccgt tcccggcgga tgttcctgaa cgccgacatc cggagatagg caggcaacac
      181 gtgtgcgtcg agcagcggta tcagaatgac cagagaaccc tgcgggggct ccccctgccg
      241 tcgaggcacc gccttttccg gcaggcgctg ctgctcgtac tcccgaacga tcttgttcac
      301 gcgctcgtcc tcgcggcgcc gttcggcttc actcaacgcc tccccgcccc tcttccggac
      361 cagtttcgag accggctggc cgccgatgtg aaaggtctcg tacgacgcgc cctggctcag
      421 cgtcgcgcgg ccctgctcat cgacctttcc cgacgacgag gtcatcgtgt acgcataccc
      481 tgcgcgtagt ggccccgccc gctgctcgct gcgtatcacc gctgacagca gggcgtcgat
      541 gtcgagaggc cgctcggccg gcaccgccgg gacgtcgaac gcctgttcgc cgagaggaac
      601 attgtgcgtg acgcgttcca cggcgatcag aaaggtctct ccattccggt gccactcgac
      661 ccggtgaggc tccatcacct gatcgaccgg acgatagtcg tcgaagaacc gttcttccag
      721 gccagcgtcc gtctgttgct cgtccttcac gagcagatag ctgctggcgt cgaagaagag
      781 cgtgcgcgtc atcgcgtcag gcgaaacggc gacgacaacg atgacgggtc gcccgcgcag
      841 ttgatcccgt cgaatgacgg agacctgatt gagcgtctcg gaatccagga gacgcgtgtt
      901 ggcgtacgtc gcttcggctc ggacacgaga gacggactgg ccgtacaacg tgcgcaggcc
      961 ctcgacgccg tcttcctgcc acgccgatcg gccgttgaac ccggcgcgcc accgggagtc
     1021 ggcccacgac atgctcatga agtaccggtc cgggcgactg gttcgttgaa tgaagacacc
     1081 cgaccgcccg cccccggaac gaatggtgcc ggagacgtcg gtgctgacga tcttctcgac
     1141 agccttcctg ccgcctatcg cctcgatgtg ccgctcgatg accttgcctg cggactgggc
     1201 ctgggccgcg atcgggtaac cgaccatcgc gatcgacacg cacagcgcgc cggacgtcaa
     1261 cctcatgacc tgtcccaaga tacctgaacg acggtgcact tacagcataa tgaacggagt
     1321 gctcacggac agccaccagc ggccgctgcg caacctccgc ctgtcggtca ccgaccggtg
     1381 caacctgcgg tgcagctact gcatgccgga accggagtac gtgtggctgc cccgcgaaga
     1441 catcctgcat ttcgaggaac tcgagatgct cgtggacgtc tttaccgggc tgggcgtgga
     1501 caaggtgcgg ctgaccggcg gcgagccgct cctccgccgg gggctccccg acctcgtgcg
     1561 ccggctcgcg gcgcggccgg caattcgcga cctcgcgatg accaccaacg gcgtgctgct
     1621 cgcggcccag gcgggcgcgt tgcgcgacgc gggcctccat cgcctcacgg tgagcctcga
     1681 cacgctgcgg ccggatcgct ttcgagatct gacccggtac gacgagctcg ggcgcgtgtt
     1741 cgacggcatt gccacggctg ctccgctctt tcccggcctc aagctcgaca ccgtcgtcat
     1801 tcgcggcgtc aacgaggacg agatggtgga tctcgtcgag ttcgcgcgcg gctacggcgc
     1861 cgagatccgg ttcatcgagt acatggacgt cggcggcgcc acgcactggt ctggctcgcg
     1921 cgtcgtgtcg cgccgggaga tgctcgacct gctcggcgcc cactacggcg agatcacgcc
     1981 ggtcgtcgag gcttcgtccg cgccggcgga tcgataccgg ctcgccgatg gaaccgtctt
     2041 cggcatcatt tcatcgacga ccgagccgtt ctgcgactca tgcgatcgaa gccggctgac
     2101 cgcggacggg ttgtggtacc tctgcctcta tgccgcgcac ggcacggacc ttcgccgccc
     2161 cctgcgcgcc ggggtttcaa ccgatcatct cgccaagctg attcgcgcga cgtgggaagt
     2221 ccggagcgac cgcggcgccg aagaacggct cgccgcgcgc gaccggtcgc cgctcatccc
     2281 ggtggatcgg ctccgccgcg accctcacct cgagatgcac ac
//
LOCUS       DBNGSH010000072         2816 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1533190, whole genome shotgun sequence.
ACCESSION   DBNGSH010000072 DBNGSH010000000
VERSION     DBNGSH010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1533190"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /gene="gyrB"
                     /locus_tag="AC16CY_01690"
     CDS             complement(<1..2134)
                     /gene="gyrB"
                     /locus_tag="AC16CY_01690"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR3352514.1"
                     /translation="MAANGPNGNGSADDYSADKIKVLEGLEAVRKRPAMYIGSTGATG
                     LHHLVYEVVDNSIDEALAGYCDQVNVTIHIDGSITVVDNGRGIPVDRHESGKSAAEVV
                     LTVLHAGGKFDNDSYKVSGGLHGVGVSVVNALSERLDLEIWRNGQVYQQSYERGTPLG
                     ELEVTGTTQRRGTKVTFKPDGQIFETTDYSFDTLAQRLRELAFLNGGIVVGIDDERDG
                     KSHKFQYDGGIGSFVQHLNKNKTVINEKPIFMKGEKDGSEAEIALQWNDGYSELVFSF
                     ANNINTHEGGTHLSGFRAALTRTINAYGVKNNLAKDLKDATISGDDIREGLTGVISVK
                     IPRPQFEGQTKTKLGNTEVKGIVEAIVNDKLSQYLEENPAVAKRIIGKAVDAARAREA
                     ARKARDLVRRKGALDGSSLPGKLADCQERDPALSEIYIVEGESAGGSAKQGRDRRFQA
                     ILPIKGKILNVEKARFDKMLSSDEIKTMIAALGCGIGSEDFDADKLRYHRVIIMTDAD
                     VDGSHIRTLLLTFFYRQMHDLIDRGHIYIAQPPLFRAKRGRTETYIKNEQDLEAFLVR
                     RAVESRVVKLADGSEMMGEVLEHQLQRTIAYRKLLQKVTRRGHPPAIVSALIEGDARD
                     KSFFEHRERLDVIAARMTTPTRTVSVFRDDEHNVFALRIDDRSQGYSRQYELGVDFIT
                     SGEYRTLAAAYREIQDIKFPVVVSSGEPA"
     gene            complement(2226..>2816)
                     /gene="dnaN"
                     /locus_tag="AC16CY_01695"
     CDS             complement(2226..>2816)
                     /gene="dnaN"
                     /locus_tag="AC16CY_01695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3352515.1"
                     /translation="GHRLALVEVQHDTGVSQEVGVILPKKTLLELGKLLSEGEGDVQF
                     ESGENHLFFEVSGRVLISRMIDGQFPAYERVIPKGNDKSIEFDRERLTSAVKRVALLS
                     NERSRAVKLEVGKGKVEVTSSSSEFGEAHEQITVDYSGPALTISFNAQYVLDFLNVVD
                     TDVVQLLLKDEVSQAVMKPVGAKGYEYTYVIMPMRI"
BASE COUNT          450 a          906 c          833 g          627 t
ORIGIN      
        1 ctgccggctc tccggacgac acgacgaccg gaaacttgat gtcctggatc tcgcggtacg
       61 ccgcggccag cgtgcggtac tcgccggacg tgatgaagtc gacgcccagc tcgtactgcc
      121 gcgaataacc ctgagagcgg tcgtcaatcc gcagcgcgaa cacgttgtgt tcgtcgtctc
      181 gaaagaccga cacggttctc gtgggtgtgg tcatgcgggc cgcgatcacg tccagccgct
      241 cgcgatgttc gaagaaagac ttgtcgcgtg cgtcgccctc gatcagcgcg gagacgatcg
      301 ccggcgggtg tccgcggcga gtcaccttct gcagtagctt gcgatacgcg atggtgcgct
      361 gcagttggtg ctccaggacc tcgcccatca tctcgctgcc gtcggcgagc ttcacgacgc
      421 gcgactccac ggcgcgtcgc accaggaacg cctcgaggtc ctgttcgttc ttgatgtacg
      481 tctccgtccg gccgcgcttg gcccggaaca gtggcggctg cgcgatgtag atgtgccctc
      541 gatcgatgag gtcgtgcatc tgccggtaga aaaacgtcag gagcagcgtg cggatgtgcg
      601 acccgtccac atcggcgtcc gtcatgatga tgacgcggtg gtagcgcagt ttgtcggcgt
      661 cgaagtcctc gctgccgatg ccgcagccga gcgccgcgat catcgtcttg atctcgtcac
      721 tgctcagcat cttatcgaag cgcgccttct cgacgttcag aatcttgccc ttgatgggca
      781 agatggcctg gaatcgccgg tcgcggcctt gcttggccga cccgcccgcc gactcgcctt
      841 cgacgatgta gatctcgctg agggcgggat cccgctcctg gcagtcggcg agcttgcccg
      901 gcaggctgct cccgtcgagc gctcccttcc gccgcacgag gtccctcgcc ttgcgagccg
      961 cttcgcgcgc ccgcgccgcg tcaaccgcct tgccgatgat gcgtttggca actgcggggt
     1021 tttcctcgag gtactgactc agcttgtcgt tgacgatcgc ctcgacgatc cccttgacct
     1081 cggtgttgcc gagtttggtt ttcgtctggc cttcgaactg cggcctcggg atcttgacgc
     1141 tgatgactcc cgtcaagcct tcgcggatgt cgtcgccgct gatggtcgcg tccttgaggt
     1201 ccttcgccag gttgttcttg acgccgtagg cgttgatcgt ccgcgtcagc gccgcccgaa
     1261 aaccggagag gtgcgtgcct ccttcgtgcg tgttgatgtt gttggcgaag gaaaacacca
     1321 gctccgagta cccgtcgttc cattgcaagg cgatctcggc ctcgctgccg tccttctcgc
     1381 ccttcatgaa gatcggtttc tcgttgatga cggtcttgtt cttgttcaga tgctgaacga
     1441 aggagccgat gccgccgtcg tactggaact tgtggctctt gccatcgcgc tcgtcgtcga
     1501 tcccgacgac aatgccgccg ttcaggaaag cgagctcgcg aagccgctgc gccagcgtgt
     1561 cgaaactgta gtcggtcgtc tcgaagatct gtccgtccgg cttgaacgtg accttcgtgc
     1621 cccgccgctg cgtcgtcccg gtgacttcga gctctccgag cggtgttccg cgctcgtagc
     1681 tctgctgata cacctggccg ttgcgccaga tctcgagatc cagtctttcc gacagcgcgt
     1741 tgacgacaga gacgccgacc ccgtggagac cacccgaaac cttgtagctg tcgttgtcga
     1801 actttccgcc ggcgtgcagc acggtcaaca ccacttcagc agcggatttt ccgctttcgt
     1861 gccgatcgac cggaatgccc ctcccgttgt cgacaacggt gatcgaccca tctatgtgga
     1921 tggtgacgtt gacctgatcg cagtagccgg ccagcgcctc gtcgatggag ttgtcgacaa
     1981 cctcgtagac gagatggtga aggccggtcg cgccggtcga cccgatgtac atcgccggac
     2041 gcttccgcac cgcctcgagg ccctcgagga ccttgatctt gtcggcgctg tagtcgtcgg
     2101 ccgatccatt gccgttgggg ccgttcgcgg cgatatcgga cgctgctgaa gcggtgggct
     2161 ggtcgatggg ttctcgagga tgttccacgc ctgttttcag cttggttgga tcgagcactc
     2221 gccgctcaga ttctcatcgg catgatgacg taggtgtatt cgtatccctt cgcccccacg
     2281 ggcttcatca ctgcctggct gacctcgtct ttgagcaaca gttgcacgac gtcggtatcg
     2341 acgacattca gaaagtcgag gacatattga gcattgaagg agatggtcag ggccggtccg
     2401 gagtagtcaa ctgtgatctg ctcgtgtgct tccccgaact ccgaactgct cgacgtcact
     2461 tcgaccttgc ccttacccac ctcgagtttg accgcgcggg accgctcgtt cgacagaagc
     2521 gccacacgct tcacggcact ggtcaggcgc tcgcgatcga attcgatgct cttgtcgttg
     2581 ccctttggta tcacccgctc gtacgccgga aactgcccgt cgatcatccg tgaaatcaga
     2641 acgcgcccac tcacctcgaa gaagaggtga ttctcgccgc tctcgaactg aacgtcccct
     2701 tccccttccg acagcagctt cccaagctcg aggagtgttt tctttgggag aatgacgcca
     2761 acttcctgcg acacccccgt gtcgtgttga acctcgacca acgccaaacg gtgccc
//
LOCUS       DBNGSH010000073         3554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2721884, whole genome shotgun sequence.
ACCESSION   DBNGSH010000073 DBNGSH010000000
VERSION     DBNGSH010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2721884"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..2636
                     /locus_tag="AC16CY_01700"
     CDS             420..2636
                     /locus_tag="AC16CY_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352516.1"
                     /translation="MTLRNGSVVWYQPQVESWTGQKEIVAWSAVSYLPIGAKEPALGT
                     IKIEGPTHVSVDERVVRMDLKITEYNFKSLSPEQVKTLANDVQSLPQNERIIDLDRIT
                     AYVAGSALQVKNVEGIKADPPKVFWAANPAILVNIDGETVWSPIKDVDLRYAVNTNWD
                     LFEYGPTKTLYLRYNNSWIQSTDVKGPWSPVDKLPDSFKKLPNDDNWKDVKEAVPGKK
                     LSAKAMPKVFVSMEPAELIAIDGNLSYLLVEGASPLLWVNNTESDLFRMGQQGDFYFA
                     VAGRWFKAASLDGPWTFATPSMPEEFKRIPIEHPRSRVLASVPGTPQATEGVLLASIP
                     RTARVNKKELKAPDVVYVGNPEFKPVEGTKGVEYAVNTDKDIVKYGDVYYMCTQGVWF
                     TGRSANGPWEVATTIPEAVYTIPSSSPVHNVTYVTVEDDDDDDEWVTMAFVAGYTGMM
                     IGWGCAVWGSGWYYPPYYGGGIYYPRPHTYGMGAWYNPHTGAYGRGVAAYGPYGGVGM
                     GASYNPRTGTYARGAAAYGPYGSRSYAEAYNPRTGAYGQTRQGSNVYGNWGTSSVQRG
                     DSWAQTAHVDNYRKGTSTSGVRTDSGAAAATRTNRQGDRSTVARTQGGDIYAGKDGNV
                     YRRNEGGGWEQSNGSGGWTPTEGGGGQGAQAARDRAAQGSGQSGNRAGTTGTTGSGWS
                     NTTQGRQLEGDRSARTSGNQRTTDRSGWSSSGGSRSTAGSYGGSRARSGGGGGRRR"
     gene            2730..>3554
                     /locus_tag="AC16CY_01705"
     CDS             2730..>3554
                     /locus_tag="AC16CY_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR3352517.1"
                     /translation="MNAEQIRLEEDRSQTRPWKKWGPYLSERQWGTVREDYSESGNAW
                     DYFSHDQARSRAYRWGEDGLAGISDDRQLLCFSVALWNGKDPILKERMFGLTNSEANH
                     GEDVKEYYFYLDSTPTHSYMKYLYKYPQAAYPYSTLVDTNKTLTRHDFEYELLDTGIF
                     DEDKYFDVFVEYAKAGAEDILVQITVHNRGPEAASLRVLPTIWFRNTWSWGEGVPRPE
                     LAQAGNAPVVNISHADLGKRFLSAEGATELLFTENETNNERLFDSPNRTRFVKDGIN"
BASE COUNT          717 a         1104 c         1201 g          532 t
ORIGIN      
        1 tcgaggcgat cgacgccaag gtgccgtcgg tgacagtaaa gactgctgac gggcgacgga
       61 tgtccttcaa ggtcgagaac gccaagaacc tcgccggcta caaggccggg gacaacgtcg
      121 aaatcaccta tacgcgggcc cttgcggtca gcgtcgaacc cgcgaaaaaa taaccgtcga
      181 taagatcggt tatgtcttgc agctcgggct ggcacggccc ccggggccgt gccagccggg
      241 ttgccggatg ggagcctttt tcaatgcaga tcaggtcacg tcgttgccct gcggtggccc
      301 tcgttctcgt tgcgtcgttg acgatccctc cgggcgtagc atccggctgg cagtcgaaga
      361 ccacgcccgc caccgtcccg ccccccggca ccaatgctga caccggctgg ccgcgagcgg
      421 tgaccctgag gaacgggtcg gttgtctggt atcagcctca ggttgaaagc tggacggggc
      481 agaaggagat cgtggcgtgg tcggcagtgt cctatctgcc catcggcgca aaggaaccgg
      541 cgctgggcac gatcaagatc gaaggcccga cgcacgtgtc ggttgacgag cgcgtcgtgc
      601 gcatggacct caagattacc gagtacaact tcaagtcgct gtcgcccgag caggtaaaga
      661 cgctcgccaa tgacgtccag tcgctcccgc agaacgagag gatcatcgac ctcgatcgca
      721 tcacggccta cgtcgccggc agcgccctgc aggtcaagaa cgtggagggc atcaaggcgg
      781 atccgccgaa ggtgttctgg gccgccaacc cggccatcct tgtgaatatc gacggcgaga
      841 cggtctggag tcccatcaag gacgtcgacc tgaggtacgc ggtcaacacg aactgggacc
      901 tgttcgagta cggcccgacc aagacgctgt atctccgcta caacaactcg tggatccagt
      961 ccacagacgt gaaggggccg tggtcgccag tggacaagtt gccggacagc ttcaagaagc
     1021 tgccgaacga cgacaactgg aaggacgtca aggaggccgt gcccggaaag aagctttcgg
     1081 ccaaggcgat gccgaaggtg ttcgtcagca tggagccggc cgagctcatt gcgatcgacg
     1141 gcaacctcag ctacctgctc gtcgaaggcg cctcgccgct gctgtgggtc aacaacaccg
     1201 agagcgacct gtttcgcatg ggccagcagg gcgacttcta tttcgcggtc gcggggcgct
     1261 ggttcaaggc cgcgagcctc gatggtccgt ggacgttcgc cacgccatcg atgccggagg
     1321 aattcaagag gatcccgatc gagcatccgc gctcgcgcgt cctggcgtcg gtaccgggca
     1381 cgcctcaggc aaccgaaggg gtcctgctcg cgtcgattcc gcggacggcc cgggtgaaca
     1441 agaaggagct caaggcaccg gacgtggtgt acgtgggcaa cccggagttc aagccggtcg
     1501 agggcaccaa gggtgtcgag tacgcggtca acaccgacaa ggacatcgtg aaatacggcg
     1561 acgtgtacta catgtgcact cagggcgtct ggttcacggg gcgttctgcg aacggcccgt
     1621 gggaagtcgc gacgacgatc ccggaggcgg tctacacgat cccgtcgagc tcgccggtgc
     1681 acaacgtcac ctacgtcacg gtcgaagacg acgatgatga cgatgagtgg gtgacgatgg
     1741 cgttcgtggc gggctatacg ggcatgatga tcggctgggg ctgcgcggtc tggggcagcg
     1801 gctggtatta cccgccgtac tacggggggg ggatctacta cccgcggccg cacacctatg
     1861 gcatgggcgc ctggtacaac ccgcataccg gcgcgtacgg gcgcggcgtc gcggcgtacg
     1921 gcccgtacgg cggcgtcggc atgggagcgt cctacaaccc acgtaccgga acctacgcgc
     1981 gcggcgccgc cgcgtacgga ccgtacggtt cccgcagcta cgccgaagcc tacaacccgc
     2041 gcacgggagc gtacgggcag acacggcagg gctcgaacgt ctacggaaac tggggcacga
     2101 gttccgtgca gcgaggcgac agttgggccc agacggcgca cgtggacaac taccgcaaag
     2161 gtacgtccac ctcgggggtt cgcaccgaca gcggggccgc cgccgcgacg cgtaccaatc
     2221 gccaggggga cagatcgacc gtcgcccgta cgcagggcgg cgacatttac gcggggaaag
     2281 acggcaacgt gtaccgcaga aacgaaggcg ggggctggga gcagagcaac ggctccggcg
     2341 gctggactcc gaccgagggc ggtggcggcc agggcgcgca ggcggcgagg gaccgcgcgg
     2401 ctcagggcag cgggcaatcg ggcaatcgcg ccggcaccac gggcacgacg ggctccggct
     2461 ggtcgaacac gacgcaggga cgtcagctcg aaggcgatcg cagcgcgcgc acgtcgggca
     2521 atcagcggac caccgatcga agcggctggt caagcagcgg cggcagccgg tccacggccg
     2581 gcagctatgg cggctcccgc gcgcgcagcg gcggcggcgg cggccgccgg cggtaaccgt
     2641 ttcgtcacga cgtctgcact ctgcgccggc gctcgctgat tgcgaacgcc ggcgttggtg
     2701 tcttcggccc gcctcgggga ggtacatcga tgaacgccga gcagatccga ctcgaagaag
     2761 accgttctca gaccagaccc tggaagaaat ggggaccgta cttgagcgag cggcaatggg
     2821 gcacggtacg cgaggactac agcgagagcg gcaacgcctg ggactacttc agccacgacc
     2881 aggcacgatc gcgtgcctac cgatggggtg aggacgggct ggcgggaatc tctgacgaca
     2941 ggcaactcct ctgcttctcg gtggctctgt ggaacggcaa ggacccgatc ctcaaggaac
     3001 ggatgttcgg gctgaccaac agcgaggcca accacggcga ggacgtcaag gagtactact
     3061 tctacctcga ctcgacgccg acgcactcgt acatgaagta cctgtacaag tatccacagg
     3121 cggcctaccc ctactccacg ctggtagaca ccaacaagac gctgacgcgc cacgatttcg
     3181 agtacgagct gctcgatacg ggcattttcg acgaggacaa gtacttcgac gtgttcgtcg
     3241 aatacgccaa ggcgggcgcc gaggacattc tcgtccagat cacggttcac aaccgcggcc
     3301 cggaggcggc gagcctgcgc gtgctaccga cgatctggtt ccgcaacacg tggtcgtggg
     3361 gagaaggcgt gcctcgtccc gagcttgctc aggcggggaa cgcgccagtg gtgaacatct
     3421 cgcacgcgga tctgggaaag cggtttctca gtgccgaagg cgcgaccgaa ttgctcttta
     3481 ccgaaaacga gacgaacaac gagcgcctct tcgacagccc caaccgcacg cggttcgtca
     3541 aggacggcat caac
//
LOCUS       DBNGSH010000074         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3401146, whole genome shotgun sequence.
ACCESSION   DBNGSH010000074 DBNGSH010000000
VERSION     DBNGSH010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3401146"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /gene="hisS"
                     /locus_tag="AC16CY_01710"
     CDS             <1..1187
                     /gene="hisS"
                     /locus_tag="AC16CY_01710"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR3352518.1"
                     /translation="LETLMGKYGDEGNQLIFKILKRGEHEASGEADLALRYDLTVPLA
                     RVVAQYQSKLPRLFKRYQIQPVWRADRPARGRFREFYQCDVDALGSTSPTVEADVCAA
                     VSDALTELGFNDFTIRINHRQLLTSILNVAGIPEALHGTALVAVDKLDKIGADGVKTE
                     MAGRGIGSDAAGKLIHLFEFLANDRLGSRSVMTDEAGGAAFENVGAIFDQAEATSAAA
                     HLRFDPSLARGLSYYTGAIMEINVPDLAGSLGGGGRYDNLVGMFLGQSVPACGFSLGL
                     ERILVVMGERGMFPANLPKAPADVMVAVFDAADTPHALRVAGLLRQSGLRVLVYPESD
                     KIGKQIKYADSLGVPFVAILGGNEIAAGTLTVKNLAAQTQATHDQAAAGGAILEGLRQ
                     RG"
     gene            1180..>1847
                     /locus_tag="AC16CY_01715"
     CDS             1180..>1847
                     /locus_tag="AC16CY_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR3352519.1"
                     /translation="MAEQLGDLTRTHTCGALRPSDVGADAVLLGWVHRVRDLGGLLFI
                     DVRDRDGVAQVVFDKDDEALMAKAKRLRSEFVVGVCGRVRRRSAETINPKIPTGEVEV
                     VVRRLVVLNEAKTPPFPVADETPVSEDVRLKYRYLDLRRPRLQGNVILRHRLTTAIRK
                     YFDANGFLEIETPILTKSTPEGARDYLVPSRVHAGEFFALPQSPQLFKQILMVSGMDR
                     YVQI"
BASE COUNT          321 a          574 c          628 g          324 t
ORIGIN      
        1 acctcgagac gctgatgggg aagtacgggg acgagggcaa ccagctcata ttcaagatcc
       61 tcaagcgcgg ggagcacgaa gcgagcgggg aagccgatct cgcgctgcgc tacgacctga
      121 cggtgccgct cgcccgcgtg gtggcgcagt accagagcaa gctgccacgc ctcttcaagc
      181 gttaccagat tcaacctgtc tggcgcgccg accggccggc gcgagggcgg ttccgggagt
      241 tctaccagtg cgacgtcgac gcgctgggct cgacctcgcc aaccgtcgaa gcggacgtgt
      301 gtgccgcggt ctctgacgcc ctgaccgagc ttggattcaa cgacttcacg atccgcatca
      361 accaccggca gttgctgacg tcgatcttga acgttgccgg cattcccgag gcactgcacg
      421 gcactgctct cgtggccgtc gacaaactgg acaagatcgg cgccgacggc gtgaagacgg
      481 agatggccgg gcgcgggatc gggagtgacg cggccggcaa gttgatacac ctgttcgagt
      541 tcctcgcaaa cgatcgtctc ggcagcagaa gcgtgatgac ggacgaggcg ggcggcgctg
      601 ccttcgagaa tgtcggggcg atcttcgatc aggccgaggc gacgagcgcg gccgcccatt
      661 tgcgttttga tccaagcctc gcgcgagggt tgtcgtacta cacgggtgcg atcatggaga
      721 tcaacgtgcc cgacctcgcc ggcagcctcg gcggcggcgg gcgctacgac aacctggtgg
      781 gcatgtttct cggtcagagc gtcccggcat gcggattctc tttgggcctc gagcgcattc
      841 tcgtggtgat gggcgagcgc ggcatgtttc ccgccaacct cccgaaggcg cctgcggacg
      901 tcatggtggc tgtcttcgac gccgcggata ctccccatgc cctccgggtt gccggattgt
      961 tacgacaatc cgggcttcgc gtcctcgtgt acccggagtc cgacaagatc ggcaagcaga
     1021 tcaagtacgc tgattccctg ggggtgccgt tcgtggcgat cctcggagga aacgagattg
     1081 cggccggtac cctgaccgtg aagaacctgg ccgcacagac gcaggcgacc cacgaccagg
     1141 ctgcggcagg cggcgcgatt ctcgagggac tgaggcaacg tggctgagca acttggcgat
     1201 ttgacgcgta cccatacgtg cggggcgctc cgtccatccg atgtcggcgc ggatgcggtg
     1261 ctgctcggct gggttcaccg ggtccgcgat ctcggcggtc tcctgttcat cgacgtgcgt
     1321 gacagggacg gcgtcgccca ggtcgtcttc gacaaggacg acgaagcgct gatggcgaag
     1381 gccaagaggc tgcggtccga attcgtcgtg ggggtgtgcg ggcgcgtgcg acgccgatcg
     1441 gctgagacga tcaacccgaa gattccgacg ggagaagtcg aggtcgtcgt ccgccgcctc
     1501 gtcgtgttga acgaggcgaa gacgccgccg ttccccgtcg cggacgagac gcccgtgtcc
     1561 gaggacgtcc ggctgaaata tcgctatctc gatctgcgcc ggcccaggct ccagggcaac
     1621 gtgattctcc ggcaccgtct gacgacggcg atccggaaat acttcgacgc gaacggcttc
     1681 ctggagatcg agacgccgat tctcaccaag tccacgccgg aaggggcgcg cgactacctg
     1741 gtgccgagcc gcgtccatgc gggcgagttc ttcgcgctgc cgcagtcgcc gcagctcttc
     1801 aagcagatcc tgatggtctc ggggatggat cgctacgtgc agatctg
//
LOCUS       DBNGSH010000075         4438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4590071, whole genome shotgun sequence.
ACCESSION   DBNGSH010000075 DBNGSH010000000
VERSION     DBNGSH010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4590071"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="AC16CY_01720"
     CDS             <1..837
                     /locus_tag="AC16CY_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SphA family protein"
                     /protein_id="HIR3352520.1"
                     /translation="YPLGMSAANSGVTGEHGFTYSNQLLFYTRDMSKDEQGNTLATGN
                     NAVLMDLNSFVWVSPRDILGGARYSATVTLPIARNELTSDIHGTLGRGSGFADSYYLP
                     FILGWNKDRAALRVLYGFLAPTGRFAPGASNNVGSGYWTSTLSSGQTFYLTEGKHLNL
                     SAFELYEFHTTQEGTGTRPGDTFNVDYSVMHTLRPSRGTWRLQVGVAGYEQRQTTANT
                     GPSIPPEQSEERYVVHAVGLASNAAFPEQRMSVGVRFFKEFASRSTFEGYSLQVSGAI
                     AF"
     gene            892..1449
                     /locus_tag="AC16CY_01725"
     CDS             892..1449
                     /locus_tag="AC16CY_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352521.1"
                     /translation="MHNQAEWTTPAAMALPKEGYFKKEEGRYGPVFPRTPACYGFSVV
                     AKVIPGREQAIRDYGKRIEEAVKADPSVLAPLRLHYLRWILFDVGSGLHFMYQGIFDT
                     DFDKYLEDAVALFVKTGITTVFVNLEGWPEDWKTNVNAVIKFFREHQCPSFLEYGEYP
                     YVTADEVKKALRLKAAFSTVLDQMQ"
     gene            1458..2783
                     /locus_tag="AC16CY_01730"
     CDS             1458..2783
                     /locus_tag="AC16CY_01730"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HIR3352522.1"
                     /translation="MLAFDDIQHILLTRAPALTGRYEFLSFNTPAAGRAWLAAIQHTI
                     QSAAAMRASVDRDTRWVTVAFTWNGLRALGVDEASLVTFPEEFKQGMVARAAMLGDTG
                     TNHPDRWVGGLASPDLHAIVILFARDAAERERCRIEHEALLARCTGVQVLSTLDLEAT
                     PPFEFAHDHFGYRDRLSQPVIEGSGDEPTPGSGAPLKAGEFILGYPDEDGPPVFQPQP
                     EILSRNGSFMAYRRLEEHVGRFRDFLAANGRTTEERELIAAKLMGRWRSGAPLVLAPD
                     ADDPTLGADAQRNNNFSYREQDPHGYAVPLGSHIRRMNPRDTAVNMNRRRMIRRGATY
                     GPPLPEGAPDDGEERGIAAFVICASLVRQFEFAQNVWVNDRNFHELGNERDPVIGLHD
                     GTLEYKIPKRPIRKTIRGLPSFTTVKGGAYFFLPGLTALRYLSALGDRD"
     gene            2800..3810
                     /locus_tag="AC16CY_01735"
     CDS             2800..3810
                     /locus_tag="AC16CY_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352523.1"
                     /translation="MTATRVGRTYNQNHVPRTYKAGKRRFSIYWTWSYPWEANRDINE
                     LDNRFSTMAEVRRSIWPNFEATQWDKMNFLQGIAGTLELFHRSTLSFQDVVGEATEHP
                     VAVYQRIDQAGYKLPIDERVLGDTDTLMVFGLDHLVSEQEAESGEIDAIRTWLTREGT
                     CLLLAPHHDVGFTSDMTQRQLEYTHHGDDLVPRQQRFGQYTRSLMKALDVPVMNQFGL
                     RPALVPGTKQLAPLAINRDLDALGLLNGVTTFNFHMHLPHYAVTENTNSARVLTRQPI
                     DLSRPHPFTDAGNREFNSCIWMPPQGTRAGHILMADSTIFTTLFGGTDSLKYFWHNVA
                     TM"
     gene            3826..>4438
                     /locus_tag="AC16CY_01740"
     CDS             3826..>4438
                     /locus_tag="AC16CY_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HIR3352524.1"
                     /translation="MELTQVAVSVAMLVFVLSSMVAMGLGLTIQQITGPLRNVRVVVL
                     ALVANFVLMPLVAVVLARVLRLDEPLGVGLLLLGAAAGAPFLPKLAQIAKGNLAFAVG
                     LMVMLMVITVAYLPLVLPVLLPGVSVNPAKIARSLVLLMLLPLAAALWVNASFPAVAT
                     RVKPLLDRASNLSLILLIVLLTVFNVGSVLAVFGTRGILAGLLF"
BASE COUNT          832 a         1451 c         1337 g          818 t
ORIGIN      
        1 tacccgctcg gtatgagcgc ggccaactcg ggtgtcacgg gggagcatgg attcacctat
       61 tccaaccaac tcctttttta cacgcgtgac atgtcgaagg acgaacaggg caacacgctg
      121 gccacgggca acaacgctgt gctgatggac ctcaactcgt tcgtgtgggt gagccccaga
      181 gacattctcg gaggcgcacg gtattccgcc acggtcacgc tgcccatcgc caggaacgag
      241 ctgacgtccg atatccacgg cacgctcggt cgagggagtg gattcgctga ttcgtactac
      301 ctgccgttca ttctgggctg gaacaaagac cgcgcggcgc tccgcgtcct ctacggtttt
      361 ctcgcaccca cggggcgctt tgctcctggc gcatccaaca acgtcggctc cggttactgg
      421 acctcgacgc tctcctcggg ccagacgttc tatctcaccg agggaaagca cctcaacctg
      481 tccgccttcg agctgtacga gtttcacacg actcaggaag gcaccggcac ccggccaggc
      541 gacacgttca acgtcgatta ctcggtgatg cacacgcttc gaccttcacg cggaacctgg
      601 aggctccagg tgggtgtcgc gggatacgaa cagcgacaga ccacggccaa cacagggccc
      661 tcgatccctc ccgaacaatc ggaggaacgc tacgtggtgc acgccgtcgg attggcctcg
      721 aacgcagcgt tccccgaaca gcgcatgagt gtcggcgtga ggttcttcaa agagtttgcc
      781 agtcgatcga cattcgaagg ctattcttta caggtgtcgg gcgctatcgc cttctgaaac
      841 gaggggacca gtccgaacga tccacaacca gcgaggatgc acatggctga catgcacaac
      901 caggccgaat ggacgacgcc cgccgcgatg gcgcttccca aggagggata tttcaagaag
      961 gaggaagggc gatacggtcc agtctttccc agaacgccag cttgctacgg gttctcagtc
     1021 gtcgccaagg tcatacctgg cagggagcag gcgatccgcg actacggcaa gcgcatcgag
     1081 gaggccgtga aggccgaccc gtcggtcctc gcgccgctgc ggctccatta cctgcgctgg
     1141 atcctgttcg acgtcggctc aggactgcac ttcatgtatc agggcatctt cgataccgat
     1201 ttcgacaagt acctcgagga cgccgtcgcg ctgttcgtca agaccggcat cacgaccgtg
     1261 ttcgtcaacc tcgagggctg gccggaggac tggaagacga atgtgaacgc cgtcatcaag
     1321 ttctttcgcg agcatcaatg cccaagcttc ctcgagtacg gtgaataccc ctacgtgact
     1381 gccgacgaag tcaagaaggc gctgagactc aaggcggcat tctcgactgt gctcgatcag
     1441 atgcagtgac accggcgatg ctcgctttcg acgacatcca gcacatcctg ctgacccgcg
     1501 cgccggcgct cactggacga tacgagttcc tgtcgttcaa cactcctgcc gccgggcggg
     1561 catggctcgc cgccatccag cacacgatac agtccgctgc cgcgatgcgg gcatcggtcg
     1621 accgcgatac acgctgggtg acggtggcct tcacctggaa cggcctgcgc gcgctcggcg
     1681 ttgacgaggc atcgctggtg acgtttcctg aggagttcaa gcagggaatg gtcgcccgag
     1741 cggcaatgct gggcgacaca gggacgaacc atcccgatcg ctgggtcgga ggcctcgcca
     1801 gccccgatct ccacgccatc gtcatcctgt tcgctcgtga tgccgcggaa cgcgagcgtt
     1861 gtcgcatcga gcacgaggcc ctgctcgcgc gctgcacagg cgtacaggtg ctctcgacgc
     1921 tcgacctcga ggcgacgccg ccgttcgagt tcgcgcacga tcacttcggc taccgcgatc
     1981 gcctctcgca gccggtgatt gaagggtcgg gcgacgaacc gacgcctggt tccggggcgc
     2041 ctctcaaagc cggcgagttc atcctcgggt atccggatga agacggcccg ccagtctttc
     2101 aaccacagcc cgagatcctg tcccgcaacg gcagtttcat ggcctaccgc cgattagagg
     2161 agcacgtcgg caggttccgc gacttcctgg cggcgaatgg ccggacaacc gaagagcggg
     2221 aactcatcgc cgcgaagctg atgggccgct ggcgaagcgg cgcgccgctc gtccttgccc
     2281 cggatgcaga cgacccgact ctcggggcag acgcgcagcg caataacaac ttcagttacc
     2341 gggagcagga cccgcacggc tacgcggtgc cgctcggatc gcacattcga cggatgaatc
     2401 cccgcgacac ggcggtgaac atgaatcgcc gccgcatgat ccggcgcggc gccacatacg
     2461 gccctcccct gcccgagggg gcgcctgatg acggcgagga gcgcggcatt gccgcgttcg
     2521 tcatttgtgc gagcctcgtc cgccagttcg agttcgcgca gaacgtctgg gtgaacgacc
     2581 ggaattttca cgaactcggc aacgagcgcg atccggtcat cggccttcac gacggtacgc
     2641 tcgaatacaa gataccgaag cgtccgatcc gcaaaaccat ccgtggactg ccctccttca
     2701 cgacggtgaa aggcggcgcg tatttctttc tgcctggcct gacggcgctg cgatatctgt
     2761 cggcgctcgg tgaccgagac tagaaggggg acactcgcca tgacagcgac tcgcgtcggg
     2821 cgtacataca accagaacca tgtaccccgc acctacaagg caggcaagcg ccggttcagc
     2881 atctactgga cgtggagcta tccatgggaa gccaaccggg atatcaatga actggacaac
     2941 cgcttctcga cgatggcgga agtccgtcgc tcgatatggc cgaatttcga ggcgacccag
     3001 tgggacaaga tgaacttcct gcagggaatc gccgggacgc tggagctctt ccaccggtcc
     3061 acactcagct tccaggatgt cgtcggcgag gccaccgaac acccggtcgc tgtctaccag
     3121 cggatcgacc aagccggtta caaactgccg atcgacgagc gcgtgctcgg cgacacggac
     3181 acgctgatgg tgttcggact ggatcatctg gtctcagagc aggaggccga gtcaggagaa
     3241 atcgacgcga tccggacatg gctcacgcgc gagggcacgt gcctgctgct cgcgccgcat
     3301 cacgacgtcg gattcaccag cgacatgacg cagcggcagc tggagtacac gcatcacggc
     3361 gacgatctcg tcccccgtca gcagcggttc ggtcagtaca cgcgctctct gatgaaagcg
     3421 ctcgacgttc cggtcatgaa ccaattcggc ttgcgtcccg cgctggttcc cgggacgaag
     3481 caactggcgc ctctggccat caaccgcgac ctggacgctc tcggcctgct caacggcgtc
     3541 acgactttca acttccacat gcatctgccg cactacgccg tgacggagaa cacgaacagc
     3601 gctcgcgttc tgacccggca gccgatcgat ctttcccggc cgcatccgtt caccgacgcg
     3661 ggcaaccggg agttcaactc gtgcatctgg atgccgccgc aaggcacgcg tgccggccac
     3721 atcctcatgg ccgattcgac gatcttcacg acgcttttcg gcggcaccga cagtctgaag
     3781 tacttctggc acaacgtcgc cacgatgtga tcgcacgccg ggcgtatgga actcacacaa
     3841 gtcgccgtct ccgttgccat gctcgtcttc gtgctctcga gcatggtggc gatgggcctc
     3901 ggcctcacga ttcagcaaat caccgggcct cttcgcaatg tccgcgtggt cgtactggcc
     3961 ctggtggcca atttcgtgct gatgcctctg gttgccgtcg ttctcgcgag agtgctccgc
     4021 ctcgacgagc ccctgggcgt gggactgctg ctgctggggg cagccgcggg ggcaccgttc
     4081 ctgccgaagt tggcgcaaat cgccaagggc aacctggcct tcgcggtcgg attgatggtg
     4141 atgctgatgg tcatcaccgt cgcctatctg cccctggtcc tgcccgtcct gctgccaggg
     4201 gtttcagtca acccggcgaa gatcgcgcgg tcgctcgtgc tcctcatgct gctgcctctc
     4261 gcagccgcgc tgtgggtgaa cgcctcgttc ccggcggtcg cgacgcgggt gaaaccgctg
     4321 ctcgatcgcg cctccaatct gagcctgatt ctgctgatcg tgctgctcac ggtattcaac
     4381 gtcggcagcg ttctggcggt cttcggtacg cgcggcatcc tggccggcct gctgtttc
//
LOCUS       DBNGSH010000076         4495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2892103, whole genome shotgun sequence.
ACCESSION   DBNGSH010000076 DBNGSH010000000
VERSION     DBNGSH010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2892103"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="AC16CY_01745"
     CDS             <1..352
                     /locus_tag="AC16CY_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HIR3352525.1"
                     /translation="VPPQDLTMSLLRDIGWYPDADVDGFADDVDECDASDLSPTVVIG
                     GTDTGVANLMFTNGCTMNDEIIAAAAAAKNHALFVSAVSQLANAWRGAGLITDQEKAA
                     IQTAAAQAGIGKSE"
     gene            complement(453..1664)
                     /locus_tag="AC16CY_01750"
     CDS             complement(453..1664)
                     /locus_tag="AC16CY_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3352526.1"
                     /translation="MLTRRSFLRASAITIGAMGAAPPWLLRAAAQAQASRKILVAVFQ
                     RGAADGLNIVVPFFEPRYYQVRPGLAVPTPGKPNGGLDLDGRFAFHPVLQPLKTFWDS
                     GQLAVVHAAGSPDSSRSHFDAQDYMECGTPGLKTDDGWLNRALPPDVAGVSPVRAIAA
                     SGAQLPRTLRGSRGAIAVDNLSRFGVSDKNTAEVLERLYSTTSDAGIRTHGKATFEAV
                     RMIESIQRRSYAPANGAQYGAAGEFGQRLQQIARLIKADAGVEVAFADIGGWDHHGNE
                     NAQLPNMLRQFSMGLAAFVRDMGDRMSDIVLVTMSEFGRTVAENGSLGTDHGHGNVMM
                     VLGGSIAGGRVYGDWPGLEPEQLFEKRDLAVTTDFRDVLGELVRGHLGQDPAKVFPGY
                     RIEKTLGLIKA"
     gene            complement(1667..3589)
                     /locus_tag="AC16CY_01755"
     CDS             complement(1667..3589)
                     /locus_tag="AC16CY_01755"
                     /inference="COORDINATES: protein motif:HMM:NF020390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HIR3352527.1"
                     /translation="MTRLGFAVGTLALLAASHVPLLASGNLFDKKLSKDKQVVHVLNR
                     LTFGPRPGDAEHVRRTGIEQWIRQQLHPELNADNALLTSHLKPLETLQLATWQILDKY
                     QPPPPVPAISPRPPLNQVLSAEQIQKLMTGTAEERTAIIMSIPADRLPTFLPGLSPDM
                     LKGLPALAEQAAKLRQLEAAARTAEFQRLNPPLQQLLTAEERRLALQGSPKEKRELLM
                     SFDAEKRRQILRQLPPQAVADLPELRRESLIARQPQQFVNQELIEGRLLRALYSSHQL
                     SEVLVDFWLNHFNVFNGKGPARMLLTSYERDAIRPHVFGRFHDLLLATARHPAMLHYL
                     DNWMSQVPRDDLPVFSTPGPQPQRRPGLNENYGRELLELHTLGVDGGYTQDDVVAVAR
                     CFSGWTIYDVNRFAEFQFNPAMHDRKEKVVLGHTIAAGGGEQDGVQVIAILARHPATA
                     RFISRKLARRFVADVPPQSLVDRMAATFTQTDGDLRAVLEAMFTSREFLSEGAWQSKL
                     KTPMELVLSAARALDADVTDAGALAQYIADLGQPLYAKTEPTGYPDTLEAWANSAGFL
                     GRMNFASALATGKVAGANIDAARLPRDPRAAMELLGAGASRTALQKALGNLGEKELPP
                     ATLAALVIASPDFQRR"
     gene            3735..>4495
                     /locus_tag="AC16CY_01760"
     CDS             3735..>4495
                     /locus_tag="AC16CY_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3352528.1"
                     /translation="MRTTPTIDRRRFLKLSSAALAALPAGCIPRPLPARSVYESLIPA
                     EPASLTVNDVHSQLNATRVAGIVKPKDVEALASAVAQARGEGRAVCVAGGRHAMGGQQ
                     FAEDALLVDTRSLNRVLSLDADRGIVEAEAGIQWPALLQELQRLQEGRSPQWGIVQKQ
                     TGADRLSLAGALACNAHGRGLALRPMIDQVESFDLLDADGAIRECSRERNRELFTMAI
                     GGYGLFGLITRVRLRLRPRTKVRRVVVLAETDGII"
BASE COUNT          742 a         1441 c         1548 g          764 t
ORIGIN      
        1 cgtgccgccg caggacctca cgatgtcgct gctgcgtgac atcggctggt atccggacgc
       61 ggacgtggac ggcttcgcgg acgacgtgga cgaatgcgac gcatccgatc tcagtccgac
      121 ggtggtcatc ggcgggaccg acacgggcgt cgcgaatctg atgttcacca acggctgcac
      181 gatgaacgac gagatcatcg ccgcggcagc cgcagccaaa aaccacgcat tgttcgtgag
      241 cgcggtctcc caactggcca acgcgtggcg cggcgccgga ttgatcacgg accaggagaa
      301 agccgcgatt cagacggcgg ccgctcaggc cggaatcggc aagagcgagt agatctccga
      361 caactcccaa cgcccaacct ccaactccca aaggaaagat gtttgggagt tgggagttgg
      421 gaattgggaa ttggagtctg ggagttgtcg gatcaggctt tgataaggcc cagcgtcttc
      481 tctatccggt aaccaggaaa caccttcgcc gggtcctgac ccaggtgccc gcggaccagt
      541 tcaccgagca cgtcgcggaa gtcggtcgtc accgcgaggt cccgcttctc gaatagctgc
      601 tccggctcga ggccgggcca gtcgccgtag acgcggcctc ccgcaatcga gccgccgagc
      661 accatcatca cgtttccgtg cccgtgatcg gtcccgaggc tgccgttctc cgcgacggtg
      721 cggccgaact ccgacatcgt cacgagcacg atgtcgctca tgcggtcgcc catgtcgcgg
      781 acgaatgcgg cgagccccat cgagaactgc cgcagcatgt tgggcagctg tgcgttctcg
      841 ttgccgtgat ggtcccagcc gccgatgtcg gcgaaggcca cttcgacgcc cgcgtcggcc
      901 ttgatcaacc tggcgatttg ctgaagccgt tggccgaact cgccggccgc gccgtattgc
      961 gcgccgttgg cgggcgcgta cgaacgccgc tgtatcgatt cgatcatgcg cacggcctcg
     1021 aacgtcgcct tgccgtgcgt cctgataccg gcgtcgctcg tcgtggaata cagacgctcg
     1081 agcacctccg ctgtgttctt gtcggacacg ccgaaccggg agagattgtc gacggcgatc
     1141 gctccacgac ttccacgaag cgtccggggc aattgggctc cggacgcggc aatggcgcgg
     1201 acgggggaca caccggcgac atccggcggc agcgcgcggt tcagccatcc gtcatcggtc
     1261 ttgaggccgg gcgtgccgca ctccatgtag tcctgcgcgt cgaagtgcga tcggctggag
     1321 tcgggcgagc ccgccgcgtg caccaccgcg agctgaccgc tgtcccagaa ggtcttcaac
     1381 ggctggagca cgggatgaaa cgcgaagcgg ccgtcgagat cgagaccgcc gttcggtttg
     1441 cccggcgtcg ggacggcaag accgggccgc acctggtagt accgcggctc gaagaaggga
     1501 acgacgatgt tcaggccgtc ggcggcgccg cgctgaaaga cggcgacgag aatcttgcgg
     1561 ctggcctgcg cctgtgcggc ggcgcgcagc agccatggtg gcgcggcgcc catggcgccg
     1621 atggtgatcg ccgaggcgcg gagaaacgag cgtcgagtca gcacggctac ctccgttgaa
     1681 agtcggggga cgcaatgacc agagcagcga gcgtcgccgg cgggagctcc ttctcgccga
     1741 ggttgccgag cgctttctgc agcgcggtgc gcgaggcgcc ggcgccaagc agttccatgg
     1801 cggcgcgagg gtcacgcggg agtctcgcgg catcgatgtt cgcgccggca accttgccgg
     1861 tcgccagcgc gctggcaaag ttcatccggc cgaggaaccc cgctgaattg gcccaggcct
     1921 cgagcgtgtc cggatatccc gtcggctccg tcttggcata gagcggctgg ccgagatcgg
     1981 cgatgtactg tgcgagcgcg ccggcgtcgg tgacgtccgc gtcgagcgca cgtgcggcgc
     2041 tgaggacgag ctccattggc gtcttcagtt tcgattgcca cgcgccttcc gagaggaact
     2101 cgcgcgacgt gaacatcgcc tcgagcacgg cccgaaggtc gccgtcggtc tgcgtgaacg
     2161 tggcggccat ccggtcaacc agcgactggg gtggaacatc cgcgacgaac cgccgggcca
     2221 gcttgcgcga gatgaatcga gccgtggcgg gatggcgggc caggatggcg atgacctgca
     2281 caccatcctg ctcgccgcct ccggccgcga tcgtgtgtcc cagcacgacc ttctccttcc
     2341 ggtcgtgcat tgccgggttg aactggaatt ccgcgaacct gttgacgtca tagatggtcc
     2401 agccgctgaa gcagcgcgcg acggcgacga catcgtcctg cgtgtagccc ccgtccacac
     2461 ccagcgtgtg cagctccagc agctcacgcc cgtagttctc gttcagtccc ggccgccgct
     2521 gcggctgcgg gccaggagtc gagaacacgg ggagatcgtc acgaggcacc tgcgacatcc
     2581 agttgtcgag gtagtgcagc atcgccgggt ggcgagccgt cgcgagcagc agatcgtgga
     2641 agcgtccgaa gacgtgaggc cggatcgcgt cgcgctcgta gctggtgagc agcattcgcg
     2701 ccgggccttt gccgttgaag acgttgaagt ggttcagcca gaagtcgacc agtacttcgc
     2761 tgagctggtg cgacgagtag agcgcgcgaa ggagccgccc ctcaatcagc tcctggttga
     2821 cgaactgctg cggctgacgg gcgatgagcg attcgcgccg gagctccggc agatcggcca
     2881 cggcctgcgg cggtagctga cgaaggatct ggcggcgctt ttccgcatcg aacgacatca
     2941 gcagctcgcg cttttccttc ggcgatccct gcagcgccag gcggcgctct tccgctgtga
     3001 gcagctgctg gagcggaggg ttgaggcgct ggaattccgc ggtacgcgcc gccgcttcca
     3061 actgccgaag cttcgccgcc tgttccgcca gcgccggcaa ccccttcaac atgtccgggc
     3121 tgaggccggg aagaaacgtg ggcagccgat cggctggaat ggacatgatg atggccgtgc
     3181 gctcttcagc ggtcccggtc atgagcttct ggatttgttc ggccgacagc acctggttca
     3241 gcggcgggcg ggggctgatc gccggcacgg gcggcggcgg ctgatacttg tccaagattt
     3301 gccaggtggc gagctgcagt gtttccaacg gcttcagatg cgatgtgagc agcgcgttgt
     3361 cggcattcag ctcggggtgc aattgctgcc gaatccattg ctcgatgccg gttcgacgaa
     3421 cgtgctccgc atcgcccggc cgcgggccga acgtcaggcg attcaggacg tggacgactt
     3481 gcttgtcttt ggagagcttc ttgtcgaaca ggttgccaga ggcgaggagc ggaacgtgag
     3541 aggcggcgag cagtgcgagg gtacccaccg cgaatcccag acgtgtcata ggatccccct
     3601 ccagcgacgt tcgccttcat tagactccac ttccggaaaa tcggttggag gccgccggtt
     3661 ttcagccttt ttcaggacgt ccgtcaggac tcttcccgat cggtccgcct acgctgtcct
     3721 cgcaggaggg ccagatgcgg accaccccca cgatcgatcg ccggcgcttt ctgaagctct
     3781 cgtcggcagc cctggcggcg ctgccggccg ggtgcatccc acggcccctg cctgcgcggt
     3841 ccgtgtacga gtcgctcatc cccgcggagc cggcgtcgct cacggtgaac gacgtccact
     3901 ctcagctgaa tgccacgcgc gtggccggca tcgtgaaacc gaaggacgtc gaggcgctgg
     3961 cgtccgccgt cgcgcaggcg cgaggcgagg ggcgcgccgt ctgcgtggcg ggcggccggc
     4021 acgcaatggg cgggcagcag ttcgccgagg atgcgctgct cgttgacacg cgttcgctga
     4081 atcgagtgct ctcgctcgac gccgatcgcg ggatcgtcga ggcagaggcg ggcattcagt
     4141 ggccggcgct gcttcaggaa ctgcagcgtc tgcaggaggg acgcagccct cagtggggga
     4201 tcgtgcagaa gcagacgggc gccgatcgcc tgagtcttgc gggagccctg gcctgcaacg
     4261 cccacggccg cggcctggcg ctgaggccga tgatcgatca ggtcgagagc ttcgacctat
     4321 tggacgccga cggcgcgatc cgcgagtgtt cccgcgagcg gaaccgggag ctgttcacga
     4381 tggcgatcgg cggctacggg ctcttcggct tgatcacacg cgtccggctg cgcctccgcc
     4441 cgcgaacgaa ggtccggcgc gttgtcgtgc tggcggagac cgacggcatc atcga
//
LOCUS       DBNGSH010000077         4970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2212869, whole genome shotgun sequence.
ACCESSION   DBNGSH010000077 DBNGSH010000000
VERSION     DBNGSH010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2212869"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..915
                     /locus_tag="AC16CY_01765"
     CDS             340..915
                     /locus_tag="AC16CY_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352529.1"
                     /translation="MPRSKARCLIALLSASLAVASCGEDGSNGNPVSSTQVSTPRLLR
                     EGSFFLAAPDEDFYYYDLVPVTDGASGRWEAAVDWALASNELWMWVATGACTTQQFES
                     PECPFEVSCPCQFAVRSEAATPKPRVLTIPNAQGGTRTLIVLNLGPREDNVTYRVTLT
                     PSTLLGSPPRSVSDDRSHARVSTGRKAVLRR"
     gene            complement(919..1437)
                     /locus_tag="AC16CY_01770"
     CDS             complement(919..1437)
                     /locus_tag="AC16CY_01770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3352530.1"
                     /translation="MSDPCNWLVDNSRLLPRAGTALDVACGRGRNALWLAGRGLKTIA
                     VDRDESAVAFVNQEATRQVLPLWAQAVDLEHGPPFLHPSSFDVVVVVHYLHRPLFPAL
                     VAALRPGGVLVYETFTRAQAARGKPTNPAFLLEPGELEKRLQPLEIVASREGDFDDRM
                     VASVIARRPREH"
     gene            complement(1434..2672)
                     /locus_tag="AC16CY_01775"
     CDS             complement(1434..2672)
                     /locus_tag="AC16CY_01775"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR3352531.1"
                     /translation="MGVVIIGAGAAGLATAIFAARSAPGLEVRCLDGARTIGAKILVS
                     GGSRCNVTNRVVTERDFWGGSPRAVRNVLRAFPADRVSGFFGSLGVALHEEEDGKLFP
                     DTNRSRTVLDALLAEAGRLGVIVETGRRVTELCRSREGFTVGTAGGMRYEAHAVVLAT
                     GGRSLPKTGSDGFGYELAKRLGHGYVETTPALAGLVLDGERHRRLAGVAHPVRLDLRA
                     DGTFTTRLDGALLWTHFGVSGPVALNMSRHWHRARLASAAIDVVLNVCPGETLESLEQ
                     WWLEQERERPRARAATVLAARLPASVAEEWTSASGIDAAATMAHLPKDDRRRLLRALL
                     ETRLDVRDSRGYAYAEVTAGGILLDEIDTATMQSRLCPGLYLVGEILDVDGRLGGFNF
                     QWAWSSGWVAGQAIAKAAAP"
     gene            2690..3979
                     /locus_tag="AC16CY_01780"
     CDS             2690..3979
                     /locus_tag="AC16CY_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR3352532.1"
                     /translation="MALLSTIHHPPTIRGDITGGITTFFTMAYIVVVNPGILASPGTG
                     MPFSGVLTATVVLACSMTLLMGLYARLPFGVAPGMGLNAFFAYTIVLQQQVPWPTALG
                     IVFWGGVLFLLVSITPLRERIAVAIPANLRLATAAGIGLLLTLIGLRNAGLVEPDPQT
                     LLRLGTLDHRAAFLLAGVVVAVVLMRRRSGLAFLASIFVVTAAAWSLGYAVPPERVVS
                     PPDFSSAFLKLDIMGALRLALLPAIVTILVTDLFDSLSTFIGVANAAGLTERDGTPLN
                     LRRGLIVDAFATLGAGLAGTSPGTAYVESIAGIRMGARTGRASIVTALCFLPCLFVSP
                     LAAAVPGYATAAVLVLVGLTMFQMVTRLDFESIEDGLPAFVTIALIPLTLSISQGILW
                     GFLLHALLYTSVGRVREVSLARWGLAALSAGLLLLGH"
     gene            complement(4079..4507)
                     /locus_tag="AC16CY_01785"
     CDS             complement(4079..4507)
                     /locus_tag="AC16CY_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3352533.1"
                     /translation="MQSMAQLLSTKGSRVWSISPDATVYEGLELMAEKNIGALLILDG
                     VRPVGIMSERDYARKIILEGRSSHETQIRSIMTTRLVSVRLDDTIEEGMAIMTRERIR
                     HLPVLDGEAVVGMVSIGDLVKAMIDRQQFTIEQLERYIAT"
     gene            4593..4769
                     /locus_tag="AC16CY_01790"
     CDS             4593..4769
                     /locus_tag="AC16CY_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352534.1"
                     /translation="MADGPSSTLACGCQVRTYRDFLGRVVGTILVKGPDCPREEHNEG
                     KTVVMPGRENARPD"
     gene            4791..>4970
                     /locus_tag="AC16CY_01795"
     CDS             4791..>4970
                     /locus_tag="AC16CY_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352535.1"
                     /translation="MVSPTDPTVLWRMSRGKSTAHATVFPGDRHTTVTWFFDGVMDRA
                     ENYDTLDLAMARADQI"
BASE COUNT          766 a         1757 c         1608 g          839 t
ORIGIN      
        1 ccagcgagcg catcgagctg acgccgtcgg cgcaggtgac cggtgacctg agcgcgacgc
       61 acgtcgtcgt cgccgacggc gcgacgttcc acggccgcat cgacatgggc cgccgcacga
      121 ttgccgccaa ggtcgcgcaa tacaaagcgg cggcgcgata gaagcagccg acgccctcgc
      181 gcgcacgtcc gcctggcgcc tccccgcatt gcccgaaagg gggccgacac aaaggctccg
      241 ccccaggcgg ctgttcgatt cgtacagtcc gagcgttgat gcaatagaag cccgcgcccg
      301 ctgcgccatc aaccagcatc cgatgaacga ggagggacca tgccacggag caaggctcgc
      361 tgcctgattg ccctgctgtc ggcgtctctg gcagttgcat catgcggaga agatgggtcc
      421 aatgggaacc cggtctcgtc gacgcaggtg tctactcccc ggttgcttcg ggaaggaagc
      481 ttctttcttg cggccccgga cgaagacttc tactactacg atctcgttcc agtgaccgac
      541 ggcgcgtccg gacggtggga ggccgccgtt gactgggccc ttgcaagcaa cgagctgtgg
      601 atgtgggtcg cgacgggcgc gtgtacgact cagcaattcg agagtccgga gtgtcccttc
      661 gaggtttcgt gcccgtgcca gttcgcggtt cgctccgagg ccgcgacacc gaagccgcgc
      721 gtgctcacga tcccgaacgc acagggcgga acccggacgc tcatcgtgtt gaacctggga
      781 ccacgcgaag acaacgtgac ctaccgtgtg acgctgacgc cttcgaccct gctcggcagt
      841 cccccgcgtt cggtttccga cgacaggagt catgcgcgcg tctcgaccgg ccggaaggcc
      901 gtactccgtc ggtgacggtc aatgctctcg gggccggcgg gcgatgacgc tcgccaccat
      961 gcgatcgtcg aagtcccctt cgcggctcgc gacgatctcg agcggctgca ggcgcttctc
     1021 gagctcgccg ggctcgagca ggaatgccgg gttggtcggc ttgccacgcg cggcctgcgc
     1081 acgagtgaac gtctcgtaca cgaggacccc gcccggacgc agcgcggcga cgagcgcggg
     1141 aaagagcggc cggtgcaggt agtgcacgac gacgacgaca tcgaaggagg aggggtgcag
     1201 aaaaggaggc ccgtgctcca ggtcgacggc ctgggcccac aacggcagca cctgcctcgt
     1261 cgcctcctga ttgacaaacg caaccgcgga ctcgtcccga tcgacggcga tcgtcttcag
     1321 cccgcggccc gccagccata gggcgttccg tccgcggccg cacgcgacgt ccagcgccgt
     1381 cccggcgcgc ggcagcagcc ggctgttgtc caccagccag ttgcacggat cgctcacggc
     1441 gcggccgcct tcgcgatcgc ctgtccggcg acccagccgg aggaccaggc ccactgaaag
     1501 ttgaagccgc ccagacgtcc gtccacgtcg aggatctccc ctaccagata taaccccgga
     1561 cacagccgcg actgcatcgt ggccgtgtcg atctcgtcca gcagaatacc gccggcggtg
     1621 acctccgcat acgcgtatcc gcggctgtct cgcacatcca gcctcgtctc gagcagggcg
     1681 cgcagcagcc gccgccggtc gtctttcggt aaatgcgcca tggtagcggc ggcgtcgatg
     1741 ccgctggccg acgtccattc ctcggccacc gacgccggca gccgcgcggc cagtaccgtg
     1801 gccgctcgcg cgcgcgggcg ctcccgctcc tgctccagcc accactgttc gagcgattcg
     1861 agcgtctcgc ccgggcacac gttcaggacg acgtcgatgg cggcgctcgc gaggcgggcg
     1921 cggtgccagt gccgcgacat gttcagcgca acgggcccgc tcacgccgaa gtgggtccac
     1981 agtagcgcgc cgtcgaggcg cgtcgtgaac gtgccgtcag ctcgcagatc gagcctgacc
     2041 ggatgggcca caccggcgag ccgccggtgg cgctctccgt cgagcacgag cccggccagc
     2101 gcgggcgtgg tctcgacgta tccgtgtccg agccgcttcg ccagctcgta tccgaacccg
     2161 tcgcttccgg tctttggcag ggagcggccg cccgtggcca gcaccacggc gtgcgcctcg
     2221 taacgcatcc cgcccgccgt cccgaccgtg aaaccctctc gggagcgaca gagctccgtc
     2281 acgcgccggc ccgtctcgac gatgacgccg agcctgccgg cttcggcaag cagcgcgtcg
     2341 agcaccgtgc gcgatcggtt cgtgtcggga aagagcttgc cgtcctcctc ctcgtgaagc
     2401 gcgacgccca gcgacccgaa gaagcccgag acgcgatccg ccggaaacgc acgcagcacg
     2461 ttccggaccg cgcgcggcga gccgccccag aagtcgcgct cggtgacgac acgattggtg
     2521 acgttgcagc gggagcctcc gctcacgagg atcttcgcgc cgatcgtccg cgcgccgtcg
     2581 agacagcgca cctcgagccc cggcgcggac cgggccgcga agattgcggt tgcgagcccg
     2641 gcggcgccgg cgccgatgat gacgactccc attggctggt ggtgagtggt tggcactact
     2701 atccactatc caccatccac caaccatccg gggcgacatc acgggcggca tcacgacctt
     2761 cttcacgatg gcctacatcg tggtggtcaa cccgggcatc ctcgcctctc caggcacggg
     2821 gatgccgttc tctggcgtcc tcacggcgac ggtcgtcctc gcctgcagca tgacgctcct
     2881 gatggggctg tacgcgcggc tgccctttgg cgtcgcgccg ggcatgggcc tcaacgcgtt
     2941 cttcgcctac acgatcgtcc tgcagcagca ggtgccctgg cccaccgctc tcgggatcgt
     3001 gttctggggc ggcgtgctct tcctgctcgt gtccatcacg ccgttgaggg agcggatcgc
     3061 cgtcgccatc cccgcgaacc tccggctcgc cacggcggca ggcatcgggc tgttgctcac
     3121 gctgatcggc ctgcggaatg cgggcctcgt cgagccggat ccgcagacgc tcttgcgcct
     3181 cggcacgctg gaccaccgtg cggcgttcct gctggctggc gtcgtggtgg cggtcgtgct
     3241 gatgcggcgc cgcagcgggc ttgcgttcct cgcgtcgatc ttcgtcgtca cggccgccgc
     3301 gtggtcgctc ggctatgccg tgccgcctga gcgcgtggtg agcccgccgg atttctcgtc
     3361 cgcgttcctg aagctggaca tcatgggagc gctgcgcctc gccctcctgc cggccatcgt
     3421 gacgatcctc gtgaccgatc tcttcgattc cctttccacg ttcatcggcg tggcgaatgc
     3481 cgccggactc accgaacgcg acgggacgcc gctcaatctg cgccgggggc tcatcgtcga
     3541 tgcgttcgcc acgttagggg cgggcctcgc cggcacgtcg ccaggcacgg cgtacgtcga
     3601 gagcattgcc gggattcgca tgggtgcacg cacgggccgc gcgtcgatcg tcaccgcgct
     3661 ctgcttcctg ccgtgcctgt tcgtgtcgcc cctggcggcg gccgtgcccg gctacgcgac
     3721 ggcggcagta ctcgtcctcg tcggcctcac gatgtttcag atggtcacca ggctcgactt
     3781 cgagtcgatc gaggacggcc tgccggcatt cgtcacgatt gcgctcatcc cgttgacgct
     3841 atccatctcg caggggattc tctggggctt cctgctgcac gcgctgctct atacgtcggt
     3901 cggccgcgtc cgcgaggtga gcctcgcccg ctggggactc gcggcgctct cagcaggcct
     3961 gctgctgctc gggcattgag agctgcgtcc gacacaccga ggcaccgaga caccgagaaa
     4021 ctcctggcgt tctctacctc tgtgccttgg agcctcggtg tgtcgtggtc gtcccacgct
     4081 aggtcgcgat gtatcgttcg agctgctcga tcgtgaactg ctgccgatcg atcatcgcct
     4141 tcacgagatc gccgatcgag accatgccga cgacggcctc cccgtccagg accggcaagt
     4201 gccgaatccg ttcgcgggtc atgatcgcca tgccctcctc gatggtgtcg tcaagacgca
     4261 ccgacacgag ccgcgtcgtc atgatgctcc tgatctgcgt ctcgtgcgac gaacggccct
     4321 cgaggatgat tttccgcgcg tagtcccgct ccgacatgat cccgacgggc ctcacgccat
     4381 cgaggatcaa gagcgcgccg atgttctttt cggccatcag ctccaggccc tcgtagacgg
     4441 tcgcatcggg tgagatggac caaacgcggg accccttggt ggacaacagc tgcgccatcg
     4501 actgcatggc tcctccttta ccgggcgtgg ccacccggac ctgccgcaga agggtgaacc
     4561 aagaggataa cccctctgta caattgccac gtatggccga cggtccctcc agcacgctcg
     4621 cctgcggctg ccaggtgcgc acctaccgcg attttctcgg acgcgtcgtg ggcacgattc
     4681 tggtgaaagg ccccgattgc ccgcgcgagg agcacaacga ggggaagacg gtcgtcatgc
     4741 ccggccgcga gaacgccagg ccggattgat acgacctcat ccgcaaccca atggtcagcc
     4801 cgaccgatcc gaccgtgctg tggcgcatgt cgcgcggaaa gtccaccgct catgcgaccg
     4861 tcttccctgg cgacaggcac accaccgtga cgtggttctt cgacggggtg atggaccggg
     4921 cggagaacta cgacacgctc gatcttgcga tggcgcgagc ggatcaaatc
//
LOCUS       DBNGSH010000078         2884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2043347, whole genome shotgun sequence.
ACCESSION   DBNGSH010000078 DBNGSH010000000
VERSION     DBNGSH010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2043347"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2204
                     /locus_tag="AC16CY_01800"
     CDS             <1..2204
                     /locus_tag="AC16CY_01800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3352536.1"
                     /translation="GRILLLTCAGIVAAVSVLATLLVAVEWHYVYVNRQNLPDLGPFT
                     RFEFPTIGRVYDAGGTPLIELAREYREITRYEDIPPIVRDAILAAEDKRFFSHNGVDY
                     VSLPRVLSKIRGRVVAERLMRGGRHDEVDGGALFPQGGSTITQQLVRGLFLRRETAQE
                     NSHQLRHPGLTAALLSSAVGARSVNMLARKREEMRLSLWLEREMRKRFGSKRRAKEEI
                     LARYASFVYMGNGQYGFARAAEFYFGRPLTSFTLNDADKAALLAGIAKSPRDYAPSAR
                     GREKVLRRRNQTLNLMRANGFISPGATTHARGRALQIVRQLEHPPLMAPTVIGHVLKE
                     LTARHPDLGVEDLLQGRIQVHVTADTRVQHIVNQALERGLRRYERRHPMARGTIQGSV
                     VVLQNRDGGILAETGGRQVVDGRPASYRDYNRVTQSLRQPGSAMKPIVYLAAFRSGAF
                     TLETLVPDEPIGVSNGETRPPKQISNYDGRFKGLIPIRQALAESRNAVAIWITGQIGI
                     ESVLRTSLSLGVRTPLQRYPTTALGASEMNLLELANAYRTMASGIVAEPYLIRTVVRD
                     SVEIVAAPRQGPVATLIAAAALSLIQEGLRGVVRLPTGTAHALAAQAFPIAVMGKTGT
                     TNEFRDAIFVGSTFGPDGITAAVRIGFDDNRSLGSRETGARLALPVFQELMLGVYRDK
                     IVGAAPEFPAQMEERIATALLRPTPPPATEDVALPDAAVVLGVPVLRLMPR"
     gene            complement(2208..2534)
                     /locus_tag="AC16CY_01805"
     CDS             complement(2208..2534)
                     /locus_tag="AC16CY_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352537.1"
                     /translation="MRNDEKVEDRQQDAERDRGSTEEHRMAAESARNDAETIREERER
                     LREAGETARAAGEVIRVAAETAREAAMAAVSATADTLHETLEHMKVVEEMRRTLRDLR
                     NVPDSN"
BASE COUNT          475 a          976 c          926 g          507 t
ORIGIN      
        1 ccgggcgcat cctccttctc acctgtgccg gcatcgtggc tgccgtgtcc gtgctcgcca
       61 cgctcctggt ggccgtcgag tggcattacg tctacgtcaa tcggcagaac ctgcctgacc
      121 tggggccctt cactcgtttc gagtttccga cgatcggccg cgtctacgac gccggcggga
      181 cgccgctcat cgagctggcc cgggagtatc gcgagatcac gcgatacgag gacattccgc
      241 cgatcgttcg tgacgccatc ctcgctgccg aggacaagcg attcttctcc cacaacgggg
      301 tcgactacgt cagcctgcct cgcgtgctct ccaagatccg gggccgcgtg gtggccgaac
      361 ggctgatgcg tggcgggcgt cacgacgagg tggacggcgg ggcgctcttc ccacagggcg
      421 ggtcaaccat cactcaacag ctcgtgcgcg gcctctttct ccggcgcgag accgcgcagg
      481 agaacagcca ccagcttcgc cacccagggc tcaccgcggc cctactgtcg tcggccgtgg
      541 gtgcacgaag cgtgaacatg ctggctcgga aacgggaaga gatgcggctc tccctgtggc
      601 tcgaacggga gatgcggaaa cgattcgggt cgaaacggcg cgccaaggaa gagattctcg
      661 cccgctacgc cagcttcgtg tacatgggca acggtcagta cgggttcgcg agagcggcgg
      721 aattctattt tggccggccg ctcacgagct tcaccctcaa cgacgccgac aaggctgcgt
      781 tgctcgccgg cattgccaag tcgccgcgcg actacgcgcc cagcgcccgg ggcagggaaa
      841 aggtcctgcg ccgacgaaac cagacgctga acctgatgcg ggcgaatggc ttcatctcac
      901 cgggggcgac gacgcacgcc agggggcgtg cgctccagat tgtgcgccag ctcgaacacc
      961 cgccgctgat ggcgcccacc gtcatcgggc acgtcctgaa ggagctcact gcccgccacc
     1021 ccgacctcgg cgtggaagat ctcctgcagg gacggatcca ggtgcacgtg acggcggaca
     1081 cccgcgtgca gcacatcgtc aaccaggcac tggagcgcgg tctcaggcgc tacgagcggc
     1141 gacatccgat ggctcgaggg acgatccagg gctcggtcgt cgtgctgcag aaccgcgatg
     1201 ggggcattct ggccgaaaca ggcggccggc aggtcgtcga cggtcgcccc gcttcctatc
     1261 gcgactacaa ccgtgtcacg cagtcgttgc ggcagcccgg atctgcgatg aagccgatcg
     1321 tgtatctcgc ggcatttcgg tccggcgcct tcaccctcga gacgctggtg cccgacgaac
     1381 cgatcggcgt ttccaacggg gagacacggc cgccgaaaca gatctccaac tacgacggcc
     1441 gcttcaaggg cctgatcccg atccgtcaag cgctcgccga gtccagaaac gccgtggcca
     1501 tatggatcac cgggcaaatc ggcattgaaa gcgtgctgcg gacgtccctg agtctcggcg
     1561 ttcggacgcc cctccaacgc tacccgacga cggctctggg tgcgtccgag atgaacctcc
     1621 tggagctggc caatgcgtac cgcacgatgg cctcgggcat cgtggccgag ccctacctga
     1681 tccggacggt cgtgcgcgat tctgtcgaga tcgtcgccgc tccccggcaa ggccccgtcg
     1741 cgacgttgat cgccgctgcc gcgctctcgc tcatccagga aggcctgcgt ggtgtcgtgc
     1801 gcctgcccac gggcacggcg catgccctgg ccgcccaggc gttcccgatc gccgtcatgg
     1861 gcaagaccgg gacgaccaac gagttccggg atgcgatctt cgtgggctcg accttcggtc
     1921 cagacggcat caccgcagcc gtcaggatcg gcttcgacga caatcgctcg cttggatcga
     1981 gggaaaccgg ggcaaggctg gcgctgccgg tgtttcagga gttgatgctc ggcgtgtacc
     2041 gcgacaagat cgtcggggcc gcgccggagt ttcccgccca gatggaagag cgcattgcga
     2101 ccgcgctgct gaggcccacg cctccgcccg caaccgaaga cgtcgccctc cccgatgccg
     2161 ctgtcgtact cggcgtgccc gtcctgcggc tcatgcccag gtagcggtca gttggaatcg
     2221 ggcacgttcc ggaggtcacg gagcgtccgc cgcatctcct cgacgacttt catgtgttcg
     2281 agcgtctcgt ggagcgtgtc ggcggttgcg ctgacggcgg ccatcgccgc ctcacgggcc
     2341 gtctcggctg cgactcggat cacttcaccg gcggctcgtg ccgtctcgcc ggcttcccgc
     2401 aggcgctcgc gctcctcccg gattgtttcg gcatcattcc tcgcggactc cgctgccatg
     2461 cggtgttctt cggtggaccc tcggtcccgc tcggcatcct gttggcgatc ctcgaccttc
     2521 tcgtcgtttc gcatagagat tttctttccc tcgatcacac gagtgttcgg cgaatcgatg
     2581 gacctctcct gtccgccgtg cttcagcgta tcagacaggg ccgcacgcgc ggtgtactca
     2641 aagtaacagc gacgaagcgg cagcctcgac aacccgtgtg cacaagggga cagacgcctc
     2701 ctctgaccgt gtctacgatg gtgtggctgg tcgaaacatc gcgggaggct ggaatgaaca
     2761 gaaacaacgt gaagggcgca gtgacggccg tggcggcgat gtctcttgtt ctggcaacgg
     2821 cggcctgggc ccaggacggt cgcaccgcgc tgaaacccgg gtggaacatg ttttcaccgc
     2881 agca
//
LOCUS       DBNGSH010000079         2266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2382723, whole genome shotgun sequence.
ACCESSION   DBNGSH010000079 DBNGSH010000000
VERSION     DBNGSH010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2382723"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="AC16CY_01810"
     CDS             <1..1455
                     /locus_tag="AC16CY_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024151.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050525 - cutinase activity [Evidence
                     IEA];
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ethylene terephthalate) hydrolase family
                     protein"
                     /protein_id="HIR3352538.1"
                     /translation="LPIILMLHGAGANEGTYLDQAEGLLMKLAEQHRYIVVSPLGFTP
                     LGAYGNPLRLPAVFGQTAAAASQRAAVTPARQRELNLSELEVMTALEIVTEEYGADRS
                     RTYLAGHSMGSGGAWHLAARYPERWRAVAPMSGPFVDQATYPFDRIRRLPIFMTEGTG
                     ATPSLEGSRVLARHMREGGFAFEYLEVDGNHGSMVPMVWPRIFEFFNRQGQGPTPPPR
                     AVVPGPHGVTAESYPTLATHTVYHPTNLDAFGPSNRLPIVAWGNGACARNGSAFAGFL
                     TQIASHGYLAIAIGPKSPLGPAPAPTIDDQLLLDAIDWAVRQNGDRSSRFYSRIDTTR
                     IAVMGQSCGGLQALAVSHDPRITTTVVWNSGALPPGSSSPALKQSAGNKDSLNRLHGP
                     VAYFIGGPTDVAYPNAEDDFARISKVPVFKGNLNVGHGGTYRQPGGGWFGEVAVAWLD
                     YQLKGSAEAAKWFVGADCRLCTDPLWAIEKKGMR"
     gene            1462..>2266
                     /locus_tag="AC16CY_01815"
     CDS             1462..>2266
                     /locus_tag="AC16CY_01815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3352539.1"
                     /translation="MSIRLIRAVISIGLLAIASDAVAQAPQEIRLWPGKAPGSENWSV
                     PEATTTSPTGDRTITNVSDPTVTVFLPAVASATGTAVVVAPGGALRLLGWDNEGVKVA
                     QWLNSKGIAALVLKYRTLQAMPGGRGRGGPPPGVAGAGAPRKELEIRNGNANPEPDDP
                     ALREVLHMGIADAQQALRLARRNAAAWGIDPARVGIIGFSAGGGIAVGTALAERSDAS
                     PDFLVSVYGPSLQDVNVPAHAPPLFIAVGSSHFNVTNGCLALFAAWKAAG"
BASE COUNT          361 a          756 c          788 g          361 t
ORIGIN      
        1 ctgccgatca tcctgatgct gcacggcgcc ggggcgaacg agggcacgta tctggatcag
       61 gccgaaggcc tgctgatgaa actcgccgag cagcaccgct acatcgtcgt ctcgccgctg
      121 ggattcacgc cgcttggcgc ctatggcaat cctcttcggc tgccggcggt cttcggccag
      181 accgcggctg cggcatcgca acgtgccgct gtcacgccgg cgcgccagcg cgagctcaat
      241 ctgagcgagc tcgaagtgat gactgcgctg gagatcgtca ccgaggaata cggcgccgat
      301 cgctcgcgca cctaccttgc cggccactcg atgggcagcg gcggcgcgtg gcacctcgcg
      361 gccaggtatc ccgagcggtg gcgcgcggtg gccccgatgt cgggaccgtt cgtcgaccag
      421 gcgacgtatc cgttcgatcg gatccggcga ctaccgattt tcatgacgga gggcaccggc
      481 gcgaccccgt cgctcgaggg cagccgtgtg ctggcgcgtc atatgcgcga gggcggtttc
      541 gccttcgagt acctcgaggt ggacggcaac cacggcagca tggtgccgat ggtgtggccg
      601 cggatcttcg agttcttcaa tcgccaggga caggggccga cgccgccgcc gagggcggtg
      661 gtgccgggtc cacatggtgt gaccgccgag tcctatccca cgctggccac gcacaccgtc
      721 tatcacccga cgaacctcga cgccttcgga ccgtcgaacc gtctgccgat cgtcgcgtgg
      781 ggcaacggcg cctgcgcccg caacggttcg gcgttcgccg gcttcctcac gcagatcgca
      841 tcgcacggct atctcgccat cgccatcggc ccgaagagtc ccttgggtcc ggcgccggcc
      901 ccgacgatcg acgaccagct cctgctcgat gcgatcgact gggctgtcag gcagaacggc
      961 gatcgatcga gccgcttcta cagcagaatc gacacgacgc ggatcgccgt gatggggcag
     1021 tcgtgcggcg gcctgcaggc gctcgcggta tcgcacgacc cgcgcatcac cacgaccgtg
     1081 gtgtggaaca gtggggcgct gccccccggg tcgtcgtcac cagccctgaa gcaaagcgcg
     1141 gggaacaagg acagcctgaa tcgcctgcac gggccggtgg cctacttcat cggcggtccc
     1201 acggacgtcg cgtatccgaa cgccgaagac gacttcgcac gcatctccaa ggtgccggtg
     1261 ttcaagggca atctcaacgt cggtcatggc gggacgtatc gccagccagg cggcggctgg
     1321 ttcggcgagg ttgccgttgc gtggctggac tatcagctca agggcagtgc ggaggctgcg
     1381 aagtggttcg tcggcgccga ctgccggctg tgcacggacc ctctctgggc gatcgagaag
     1441 aaaggcatgc ggtaggcgac cgtgtcgatc cgcttgatcc gcgccgtcat cagtatcggg
     1501 ctgctggcga tcgccagtga cgccgtcgcg caggcgccgc aggagatccg tctgtggccc
     1561 ggcaaggctc cggggtctga gaactggagc gttccagagg ccacgacgac ctcacccacg
     1621 ggagaccgga cgatcaccaa cgtcagcgat ccgacggtga cggtattcct tccggcagtg
     1681 gccagcgcca cgggaaccgc tgtcgtcgtc gctcccggcg gagccctgcg actgctcggc
     1741 tgggacaacg aaggcgtcaa ggtcgcgcag tggttgaaca gcaaaggcat tgcggcgctg
     1801 gtgctgaagt accggacgct gcaggcgatg cctggcggcc gcgggcgtgg aggaccgccc
     1861 ccgggtgtcg ctggcgcggg cgcgccgaga aaagagctgg agattcgcaa cggcaatgcc
     1921 aatcccgagc ccgatgatcc ggcgttacgg gaggtgctgc acatggggat cgccgatgcg
     1981 cagcaggcgc tgcgcctggc gcggcggaac gcggcggcct gggggatcga ccccgcacgc
     2041 gtcggcatca tcggattctc tgccggcggg ggcatcgcag tcgggactgc gctggcagag
     2101 cgatcggacg cgtcgccaga cttcctcgtg tcggtctacg ggccctcgct gcaggacgtc
     2161 aacgtgcccg cgcacgcgcc gccgctcttc atcgcggtcg gttcgtccca cttcaatgtg
     2221 accaacggct gcctggcgct cttcgccgcg tggaaggccg ccggca
//
LOCUS       DBNGSH010000080         1974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4420269, whole genome shotgun sequence.
ACCESSION   DBNGSH010000080 DBNGSH010000000
VERSION     DBNGSH010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4420269"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /gene="nrfD"
                     /locus_tag="AC16CY_01820"
     CDS             <1..1064
                     /gene="nrfD"
                     /locus_tag="AC16CY_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3352540.1"
                     /translation="CAEVITVFALMIGAMFPIIHLGRPWLAFWLFPYPSERQIWPNFR
                     SPLVWDFFAINTYLTGSVLFLFLPMIPDFAVVRDKSTGLRRRIYGILALGWRGTTQQW
                     HRLETAMQIMAIAIIPVAVSVHTIVSFDFSMAPVPMWQSTIFGPYFVAGAIFSGIAAL
                     LIAMAILRRFLHLERYLHPVHFENLGKLLLVMALLWGYFVFNERLVTYYGNEPSEMAV
                     FWRTQRDSFAPLYWTMVVCNFVVPLVLMGIRRFRTITGCVLASVCVVAGMWLERFLII
                     VPSLGHKYLPYSFGIYRPQPVEIIIAGSTFAAMTLLYVLFAKFVPIISIWELKAGDHP
                     PPSLIPPATEEHPLGELHL"
     gene            1106..1609
                     /locus_tag="AC16CY_01825"
     CDS             1106..1609
                     /locus_tag="AC16CY_01825"
                     /inference="COORDINATES: protein motif:HMM:NF023249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HIR3352541.1"
                     /translation="MKALYGLYPDGASAQQAVNRLRAAGASDDDITVMSPQPMEDFEF
                     GRTHKATWMWWFACGGGLTGMASGLGLSWLTETSWPIDVGGLPIYAWWPNLIITFELT
                     MLGAILATVATLVVTARLGRGSTFYDPAVSDGKILVGVADPRDSAVAGFEAALAAPAG
                     GEVKTLR"
BASE COUNT          277 a          644 c          653 g          400 t
ORIGIN      
        1 gatgcgcgga agtcatcacc gttttcgcgc tgatgatcgg cgcgatgttt ccgatcatcc
       61 acctcgggcg cccgtggctc gccttctggc tgtttcccta tccgagcgag cgccagatct
      121 ggccgaactt ccgctcgccg ctcgtgtggg acttcttcgc gatcaacacc tacctgaccg
      181 gaagcgtgct cttcctgttc ctgccgatga ttcccgattt cgccgtcgtc cgcgacaagt
      241 ccaccgggct ccggcggcgg atctacggca ttctggcgct tggctggcgg ggcacgacgc
      301 agcagtggca ccggctcgag acggccatgc agatcatggc gatcgccatc atcccggtgg
      361 ccgtctcggt gcacacgatc gtgtcgttcg acttctcgat ggcccccgtg ccgatgtggc
      421 agtccacgat cttcggtccc tacttcgtgg ccggcgcgat cttcagcggc atcgccgcgc
      481 tgctcatcgc gatggcgatt ctccgccgtt tcctgcacct cgagcgctac ctgcatccgg
      541 tgcacttcga gaatctggga aagctcctgc tggtgatggc cctgctctgg gggtacttcg
      601 tcttcaacga gcgcctcgtc acctactacg gtaacgagcc ctccgagatg gcggtcttct
      661 ggcggacgca gcgcgactcg ttcgcgccgc tctactggac gatggtcgtc tgcaacttcg
      721 tggtgccgct cgtgctcatg ggcatccggc gtttccgcac gatcaccggc tgcgtcctgg
      781 cgtcggtgtg cgtcgtcgcc ggcatgtggc tcgagcggtt tctcatcatc gtgccctcgc
      841 tcgggcacaa gtatctgccg tacagcttcg gcatctatcg tccccagccg gtcgagatca
      901 tcattgccgg ctcgacgttc gcggcgatga cgctcctgta cgtgctgttc gccaagttcg
      961 taccgatcat ttcgatctgg gagctgaagg cgggcgatca cccgccgccg agcctgatcc
     1021 cgccggccac cgaagagcat ccgctcgggg agctgcacct gtgacccttc gagagcctca
     1081 gggccacccc gagcacggtc gaggggtgaa ggccctttac ggtctgtacc ctgacggcgc
     1141 ctcggcgcag caggcggtca accgtttgcg cgcggccggc gcctccgatg acgacatcac
     1201 cgtgatgtct ccgcagccga tggaggactt cgagttcggt cgcacgcaca aggcgacgtg
     1261 gatgtggtgg ttcgcgtgcg gaggggggct gaccgggatg gcctccgggc tcgggctgtc
     1321 gtggctcacc gagacgtcgt ggccgatcga cgtgggcggg ctgccgatct acgcgtggtg
     1381 gcccaacctg atcatcacct tcgagctgac gatgctgggc gcgatcctgg cgacggtggc
     1441 gacgctggtc gtgacggcgc gcctgggccg cggatcgacc ttctacgatc ccgcggtgtc
     1501 ggatgggaag atcctggtcg gcgtcgccga tccccgtgac tccgcggtcg ccggcttcga
     1561 ggcggcgctt gcagcgcccg cgggggggga agtgaagacg ctccggtaga tgtatgtcgt
     1621 tcacacttct cggttcccct tgtgggctcg cgcgcctccg cgcggaggtc cgaccatcgg
     1681 gctggctggc gccttcggcg tggacaactc ctgcaacgcc tgctcgtcgg tacgagcgcg
     1741 cgcgacgccg gtgtggtcgc tgcggtacga gtagcggaca ggtcgagcgg cggtacaatc
     1801 agggagatgg agcccacgct cgccagcttt cttcagggcc tcgaggaggc gactgagttc
     1861 gccaaggctt atcgcttcga gatgaccgac gattaccggt ctctgatcga gcaggtcgag
     1921 gcattgccgc gcaaccaatc cggggccgac aagagtggtc ggtggcttgg aggc
//
LOCUS       DBNGSH010000081         6412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1024043, whole genome shotgun sequence.
ACCESSION   DBNGSH010000081 DBNGSH010000000
VERSION     DBNGSH010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1024043"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="AC16CY_01830"
     CDS             <1..1175
                     /locus_tag="AC16CY_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352542.1"
                     /translation="VWGSHDVHRPVFIRLLTAFDIEAFSGASYPFIVFTTACHVATGW
                     LLWRECQAGVHGGFGRALGRLVLMLVFTSVAAVACAVPLNNGYLHVLTCAVLAIVLFD
                     GADDDGRAGRRWVHWRRAGAVLAAIAAPFASAVGWAVWPILFWIAWRGRAGRAWIAAI
                     AAVATAFVLVYLQGLSIGQAAGPSPARALDSLGSLLGRLNYLITYLGLPWTRAAALTV
                     PGRVCGAVLLGAGVWAVLARGVLRAPSGRLERIGLALIMFSLGTALLAAIGRVEVAPD
                     DGVLVPVRYSSLVAPLYIGLLWVVSPALQRLWNDAGRSRIVATGVVGACVLLLALQVA
                     SGQAAAAITSRMRATIEQFEAGQTTPEMSDVVFSDVEQARREWEIIRSAGLYLNVK"
     gene            complement(1181..3775)
                     /locus_tag="AC16CY_01835"
     CDS             complement(1181..3775)
                     /locus_tag="AC16CY_01835"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR3352543.1"
                     /translation="MVRVTRALLLTTAILLAVLPIGAQRLVGGVVPEHYSLWFAPDLE
                     RETFRGRASIDVVLTEPSSSITLHAAEIEFADVTITSGGQTQTARVTTDPKTETATLT
                     VPRSIAEGRATIRIAYNGLLNDKLRGFYISRANGRKYAITQLEATDARRAFPSFDEPA
                     HKATFDISMMIDAGDTAISNGRVLSDTPGPEPGKHTLTFARTPKMSTYLAAMIVGDFV
                     CRDGASDGTQIRVCSTPDKLGLTAFALEAAEQQLAFFNAYFGIKYAFGKLDIIAVPDF
                     AAGAMENAGAITFRERLLLVDPSTSSLDARKRVAAIIAHEIAHQWFGNLVTMKWWDDI
                     WLNEGFATWMETKPVAVWKPEWRVELNEAGDTRGALGIDAMRSTRPVRTKVETAAEIN
                     QVFDGIAYEKTAALLRMIEAYVGPEAFRKGVASYLKRFAYANAAGEDFWQEMAAVTGK
                     PVDRILRSFVDREGAPVLSVRSSCSSGTGEIEVRMERFIGEPGAPSSPQTWTLPACFK
                     TPEGQPRCELIDRQRQTLRGGSCQQSFANANGRGYYFTDYPTESVGSLSSSGNLTPIE
                     RLGLLGDEWWMVRAGRHDIDAYLAAATAFARDDTSAVIEAIAGPVTSIGDTLVSAADR
                     PRYESWIRAQFGGQLAAMGLAPALGESDERQSRRAILFQLVGIAGNDPAVQRAARELA
                     AAYLKNPSSLSGTMAPGVLRVAAFGGDRALYDQYVEQLGRLDSQPELYFRLFNALPWF
                     KDAELTGRTLQFAVSPAVRTQDTATLLGLLMTRPWSQDQAWSFTQSHWTTLIARLGEF
                     QGIPSIAESLGAFCTTARAREIRGFFEKNPVPSSDRAIRQSIERIENCAALVERQRKP
                     LAAWIGRQ"
     gene            4196..4660
                     /locus_tag="AC16CY_01840"
     CDS             4196..4660
                     /locus_tag="AC16CY_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352544.1"
                     /translation="MASDTSKIEDPVRAELRQAVSVDGTTVLPVGTELVGTVTDAERS
                     ARVKGKARIAYRFHSLSYDDERYDISTALIAHEAKAQKAKDMTKVGIGAGVGAAVGGL
                     LGGGSGAAKGAAIGGAAGTGAVLATRGEEVRLGPGADVTTRLTEPLTVRVKI"
     gene            complement(4638..5525)
                     /locus_tag="AC16CY_01845"
     CDS             complement(4638..5525)
                     /locus_tag="AC16CY_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3352545.1"
                     /translation="MPGPLLSRVRRLAGLVTTVVVFFVLAGATYQGVATSLERRDFQR
                     PGGLGDVGGFQLHIYCVGTGSPTVVLEAAAGSLSAAWGQVQEDVARMTRVCSYDRDGL
                     GWSETRDGRYEPSRVPAELRALLEEAHEPAPFVLAGHELGAAFARAFAATYPRDAAAL
                     VLIDDPITGGRSSPLPPMPGAWPWLARTGLLRATGSLSRHANGLPGKAGGAMRAFLNR
                     PDHLTRAATEISRREEVEAAVRTLQLDPSVAVVEVATSAAGTPAMIVTDEDTARVTAA
                     LEAAVTRVRQARAKSSLEQ"
     gene            complement(5525..>6412)
                     /locus_tag="AC16CY_01850"
     CDS             complement(5525..>6412)
                     /locus_tag="AC16CY_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3352546.1"
                     /translation="ETTVLLTGESGTGKEVVARFIHHASRRGQGPFVAINCAALPDQL
                     LESELFGHERGAFTGAVSTKPGRIEQASGGVLFLDEVGEMAPTVQAKLLRVLEEREFM
                     RLGSTRVLHADIRVIAATNRNLHAAMQRGEFREDLYYRLGVFEIALPPLRARLDDVLE
                     LADTFLAEIGETVGRPAAGMSREAREQLLAYAWPGNVRELRNAIERAVILADGGLIRA
                     EHLPVSQYGSTEARREEQSGESLPVGGVNLEAIERSLVVKALTQARHNKTRAAKLLGL
                     TRAQLYSRIEKYGLTGTES"
BASE COUNT          985 a         2095 c         2255 g         1077 t
ORIGIN      
        1 acgtgtgggg ttcgcacgac gtgcatcggc ccgtcttcat ccggcttctg accgccttcg
       61 acatcgaggc gttttcgggc gcgtcgtatc ccttcatcgt gttcacgacg gcctgccacg
      121 tggcgaccgg ttggctgctg tggcgcgagt gccaagccgg agtccacggc ggcttcggcc
      181 gcgctcttgg acgactggta ctgatgctgg tcttcacgag cgtggcggcg gtggcctgcg
      241 cggtcccgct caacaacggt tacctgcacg tgctgacctg tgccgtgctt gcgatcgtct
      301 tattcgacgg ggccgacgac gatggacgtg cgggccggcg atgggtccac tggcggcggg
      361 ccggcgccgt gcttgccgcc atcgccgcgc cgtttgccag cgcggtcggc tgggcggtct
      421 ggccgatcct gttctggatt gcatggcggg gccgcgcggg acgcgcctgg atcgccgcga
      481 tcgccgcggt cgcgaccgcg ttcgtgctcg tgtacttgca ggggctgtcc atcgggcagg
      541 ctgccggacc gtcgcccgcg cgggcgctcg actcgctcgg ctcgctgctc ggccgactca
      601 actacctcat cacctacctt ggcctgccgt ggaccagggc cgctgcgctg accgtcccgg
      661 gacgcgtctg cggcgcggtg ctgctgggcg cgggcgtctg ggccgtgctg gcgcggggcg
      721 ttcttcgcgc cccgtcggga cggctcgagc gcatcgggct ggcgctgatc atgttttcgc
      781 tgggaacggc gctgcttgcc gcgatcggtc gcgtggaggt cgcgccagac gatggcgtgc
      841 tcgttcccgt ccggtactcg tcgctcgtgg cgcccttgta tatcgggctg ctgtgggtcg
      901 tgtcaccggc gttgcagcgt ctgtggaacg acgccggccg ctcgaggatc gtggcgacgg
      961 gcgtcgtcgg tgcgtgcgtc ctgctcctcg ccctgcaggt cgcctcgggt caggcggccg
     1021 ccgcgatcac cagccgcatg cgcgcgacca tcgagcagtt cgaggcaggg cagacgaccc
     1081 ccgagatgag cgacgtcgtg ttcagcgacg tggaacaggc gcgccgggag tgggagatca
     1141 tccgcagcgc gggactgtat ctgaacgtga agtagacggc ctactgtctc ccgatccacg
     1201 cagccagggg cttccgctgc cgctcgacaa gcgccgcgca gttctcgatg cgctcaatcg
     1261 attgacgtat cgcgcggtcg gaggagggga cagggttctt ctcgaagaac ccacggatct
     1321 cgcgggcgcg agccgtcgtg cagaacgcgc cgagcgactc cgcgatgctc gggattccct
     1381 gaaactcgcc aagcctcgcg atcagcgtcg tccagtggga ctgcgtgaac gaccacgcct
     1441 gatcctggct ccagggcctg gtcatcagga ggccgaggag cgtggcggta tcctgcgtgc
     1501 gcacggccgg agagacggcg aactgaagcg tgcgtcccgt caactcggca tccttgaacc
     1561 aggggagcgc gttgaaaaga cggaagtaca gttcgggctg cgagtcgagg cggccgagct
     1621 gctcgacgta ctggtcgtag agcgcgcgat cgccgccgaa ggcggcgacg cgcaggacgc
     1681 ctggcgccat cgtgccggac aacgaggagg ggttcttcag gtacgcggcc gcgagctccc
     1741 gggcggcgcg ctggaccgcc ggatcgttgc cggcgatgcc gacgagctga aacagaatgg
     1801 cgcgccggct ctggcgctca tcgctctccc cgagcgcggg ggcgagaccc atcgcggcca
     1861 gttgaccgcc aaactgagcg cgaatccacg actcgtatcg tggccggtcg gcggccgaca
     1921 cgagcgtgtc gccgatggac gtcaccggcc cggcgatagc ctcaatcacc gcgctggtgt
     1981 cgtcgcgggc gaaagcagtg gcggcggcga ggtacgcgtc gatgtcgtgg cgtcccgcgc
     2041 gcaccatcca ccactcgtcc ccgaggagac cgagacgttc gattggcgtc aggttcccgc
     2101 tggatgacaa cgagcccacc gactccgtgg ggtagtccgt gaagtagtag ccgcgtccgt
     2161 tcgcgttggc gaacgactgc tggcaactgc cgccgcgaag ggtctgcctc tgccgatcga
     2221 tgagctcgca gcgcggctga ccctccggcg tcttgaagca cgccggcagc gtccaggtct
     2281 gcggagagga gggggcgccg ggctcgccga tgaaccgctc catgcgcacc tcgatctcgc
     2341 ccgtgccgga gctgcacgag ctcctcaccg acaggacagg cgcgccctct cgatcgacga
     2401 agctgcgaag aatccgatcg acaggcttgc cggtgacggc cgccatctcc tgccagaagt
     2461 cctcgccggc cgcgttggcg taggcgaacc gcttcaagta cgacgcgaca cccttccgga
     2521 acgcctcagg cccgacgtat gcctcgatca tccgcagcag cgcggccgtc ttctcgtagg
     2581 cgatcccgtc gaagacctgg ttgatctccg ccgccgtctc gaccttcgtc cggacggggc
     2641 gcgtggaccg catcgcgtcg atgccgaggg cgccgcgcgt gtcgccggct tcattcagct
     2701 cgacgcgcca ctcgggcttc cagacggcga caggcttggt ctccatccag gtggcgaagc
     2761 cttcgttgag ccagatgtcg tcccaccact tcatcgtgac gaggttcccg aaccactgat
     2821 gagcgatctc gtgagcgatg atggcggcga cccgcttgcg cgcgtcgagc gacgaggtcg
     2881 agggatcgac gagcagcagg cgctcgcgaa acgtgatcgc cccggcgttt tccatcgcac
     2941 cggccgcgaa gtctggcacg gcgatgatgt ccagcttgcc gaaggcgtac ttgatcccga
     3001 agtaggcgtt gaagaatgcg agctgctgct cggcggcctc gagcgcgaat gccgtcaggc
     3061 ccagcttgtc cggcgtggag cacacccgga tctgcgtacc gtcggaagcg ccatcgcggc
     3121 agacgaagtc gccgacgatc atcgcggcca ggtacgtgga catcttcggc gtccgcgcga
     3181 acgtgagcgt gtgcttgcct ggctccggcc ccggcgtgtc ggacaacacc cgtccgttcg
     3241 agatcgccgt gtcgcccgcg tcgatcatca tggagatgtc gaaggtggcc ttgtgggctg
     3301 gctcgtcgaa ggaaggaaac gcccggcgag catcggtggc ttccagttgc gtgatcgcgt
     3361 acttccggcc gttggccctg ctgatgtaga agccgcgcag cttgtcgttg agcaggccgt
     3421 tgtacgcgat tcgaatcgtc gccctgcctt ccgcgatcga acgcgggacg gtcagggtcg
     3481 ccgtctcggt cttggggtcg gtggtcacgc gagcggtctg cgtctgtccg cccgaggtaa
     3541 tcgtcacgtc ggcaaactcg atctccgccg cgtgcagcgt gatcgacgaa gaaggctcgg
     3601 tcaggacgac gtcgatcgaa gcgcgcccgc ggaacgtttc tctttcgagg tccggcgcga
     3661 accacagcga atagtgctcg gggacaacgc cgccgacgag acgttgcgcg ccgatcggca
     3721 gcacggcaag aagaatggct gtggtgagga ggagcgctcg tgtaactctg accatctctt
     3781 caaggtagca gagttgtaac gggtggtacg gacggtgtga cgagacccgg caatacgtgg
     3841 gttgccggtt ggcaacaatc tcgcatagtc gagctgcatc ggcgggcact gcccgcaggg
     3901 cgtgccccgg aactgtcact gaaccaacgg tttcagtggg atgcggggtc cggggcgtgg
     3961 ccggtttccg gccgaggagg tttccgtgac gagatggttg tgcacgctca ctctggcggg
     4021 gttttgcgcg gcttgcacag gatctgacac gaccgagcct gtcgagagct cagctgacgc
     4081 gacgtccgcg gctgtcccgg catcggaagc ggctgttcca cgagcacggg cgccgagatt
     4141 ccgcgaagtg acgctaccgg cgggcacgac gcttcggttg gatctcagga gctcggtggc
     4201 ctccgacacc agcaagatcg aggacccggt tcgcgccgag ttgcgacagg cggtcagcgt
     4261 tgacgggaca accgtgctgc cggtgggcac cgagctcgtg ggcacggtta ccgatgccga
     4321 gcgctccgcg cgtgtgaagg ggaaggcgcg aatcgcgtat cgattccact cgctcagtta
     4381 cgacgacgag cggtacgaca tatcgaccgc tctgatcgct cacgaggcca aggcgcaaaa
     4441 ggccaaggac atgacgaagg tcggcatcgg cgccggcgtc ggcgccgcgg tgggcggcct
     4501 gctcggcggc ggaagcggcg ccgccaaggg ggccgcgatc ggcggggctg ccggcaccgg
     4561 cgcggtcctg gcgacgcggg gagaagaggt tcgtctcggg ccgggcgccg atgtcacgac
     4621 gcggctgacc gaaccactca ctgttcgagt gaagatttag cccgggcttg acgcacgcgg
     4681 gtcacggccg cctcgagggc ggccgtgact cgcgccgtat cctcgtcggt cacgatcatg
     4741 gccggcgtac ctgcggcgct tgttgcgacc tccacgactg caacggaggg gtcgagctga
     4801 agcgttcgaa ccgcggcctc tacttcctcc ctgcgcgata tctcggtggc ggctctcgtc
     4861 aagtgatcgg gccgattcag aaacgcgcgc atcgcaccgc cggccttgcc cggcagtccg
     4921 ttcgcgtgcc gtgaaagact gccggtggcg cggagtaatc cggtcctggc cagccacggc
     4981 catgcacccg gcatcggcgg aagtggggac gaccggccgc ccgtgatcgg atcgtcgatg
     5041 agcacgagtg cggctgcgtc tcgcggatac gtcgcggcga acgcccgcgc gaatgccgcg
     5101 ccgagctcgt ggcctgccag aacgaacggg gccggctcgt gcgcctcttc gagcagcgca
     5161 cggagttcag cggggacgcg cgaaggctca taccgtccgt ctcgcgtctc gctccagccg
     5221 agcccgtccc tgtcgtagct gcagacgcga gtcatgcgtg cgacgtcctc ctgcacctga
     5281 ccccatgccg ccgaaaggct gccagcagcg gcttcgagaa cgacggtcgg gctgccggtg
     5341 ccgacgcagt agatgtggag ctggaaaccg cccacgtcgc cgagccctcc gggacgctgg
     5401 aagtcgcgtc gctcgagcga agtggccacg ccctggtagg tcgcgccggc cagcacgaaa
     5461 aagacgacga cggtcgtgac caaccctgca agccggcgta cccgggacag caacggcccg
     5521 ggcactacga ctcggtcccc gtgaggccgt acttttcgat gcgggaatag agctgtgccc
     5581 gagtcaggcc cagcagcttg gctgcgcgcg tcttgttgtg cctggcctgg gtgagggcct
     5641 tgacgacgag cgagcgttcg atcgcctcga ggttgactcc accaaccggc agcgattcac
     5701 cggactgctc ctcgcggcgg gcctcggtcg atccgtattg cgagaccggc agatgttcgg
     5761 cccggatgag gccgccatcg gcgaggatga cggcccgttc gatcgcgttg cgcagctcgc
     5821 ggacgttgcc gggccaggcg taggccagca gctgttcccg ggcctcgcgc gacatgccgg
     5881 ccgcggggcg gccgacggtt tcgccgatct ccgccaggaa cgtgtccgcc agctccagga
     5941 cgtcgtccag gcgcgcacgc agcggaggca gcgcgatttc gaacacgccg agccggtagt
     6001 agagatcctc acgaaactcg ccgcgctgca tggccgcgtg caggttgcgg ttggtggccg
     6061 cgattacccg gatgtccgcg tgcagcacgc gcgtgctccc gagacgcatg aactcgcgct
     6121 cctcgaggac gcgaagcagc ttggcctgga ctgtcggcgc catctctccg acttcgtcga
     6181 ggaacagcac gcctccactg gcttgctcga tgcggcccgg cttggtcgag acggcgccgg
     6241 tgaaggcgcc gcgttcgtgg ccgaacagct ccgattccag cagttgatcg ggcaacgccg
     6301 cgcagttgat cgcgacaaac ggtccctgac cgcgccgcga cgcgtggtgg atgaaccggg
     6361 cgacgacttc cttgccggtg ccggattcgc cggtcagcag aaccgtggtt tc
//
LOCUS       DBNGSH010000082         1673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_854663, whole genome shotgun sequence.
ACCESSION   DBNGSH010000082 DBNGSH010000000
VERSION     DBNGSH010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_854663"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="AC16CY_01855"
     CDS             complement(<1..407)
                     /locus_tag="AC16CY_01855"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3352547.1"
                     /translation="MKQSGIIAVALLVFGATLAAHHSAVQFDFTRTVSVTGVVMKFRA
                     INPHMQLVLHVKDVKGERDIEFEGHSTNNMYRSGYRDNMIKVGDTITVSIAPLRTGAD
                     GGYVTAAVTASGAKFGMGSGASAADRAREREKA"
     gene            complement(434..1168)
                     /locus_tag="AC16CY_01860"
     CDS             complement(434..1168)
                     /locus_tag="AC16CY_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352548.1"
                     /translation="MHRGFRAAVGIAIFLGFGSTASAQQPDFTGVWTTYNEPGAARGR
                     GAGRGAGPGLPMTDVAKQKVEKYRALVAPTGDTPGGFCLGSGMPGSMLGSGGYPMEIH
                     QRPEQIIIVYEAHNEVRRVYLGNRIVPVADRLPGRNGHSSGRWEGNTLVVETTNLVEQ
                     VDQRYAHSDQARIVERYHLEKGAAGQDVLVAEMTMTDPVFYTAPVSEVKKWARVPNGH
                     LLPYECAEEEWLKHLDRLEKNAAAKR"
     gene            complement(1279..>1673)
                     /locus_tag="AC16CY_01865"
     CDS             complement(1279..>1673)
                     /locus_tag="AC16CY_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352549.1"
                     /translation="EDLTYFRQIYTDGRPLPADPEPAFSGHSIGRWDGDTLVVEVTGF
                     KDNGWLDAFGHPHSDALRVTERFHRLDFGTMEVEITVEDPKIYSRPFTFKFKQLLLPD
                     TDLIESFCENEKNRDRLDRLRDAAQAKN"
BASE COUNT          263 a          562 c          518 g          330 t
ORIGIN      
        1 tcggccttct cccgctcgcg cgcgcgatcg gcggcggagg cgccggagcc catgccgaac
       61 ttcgcaccgc tggcggtgac ggcggccgtc acgtatccac cgtcagcgcc cgttctgagc
      121 ggcgcgatcg agaccgtgat cgtgtcgccg accttgatca tgttgtcgcg gtagccggac
      181 cggtacatgt tgttggtgct gtggccttcg aactcgatgt cgcgttcgcc cttcacgtcc
      241 ttgacgtgca gcacgagctg catgtgcggg ttgatggcgc ggaacttcat gacgaccccg
      301 gtgaccgaca ccgtcctggt gaagtcgaac tggacggccg aatgatgcgc cgcgagcgtc
      361 gcgccgaaga cgagaagtgc caccgcgatg atgccggact gtttcatgac atgcgctccg
      421 tgtgtgtctg acgctaccgc ttggccgccg cgttcttctc gagccgatcc aggtgcttga
      481 gccattcctc ttcggcgcac tcgtagggca gcaggtgccc gttcggcacg cgcgcccact
      541 tcttcacctc gctgaccggc gccgtgtaga agacaggatc ggtcatcgtc atctcggcga
      601 ccaggacgtc ctgtccggcg gctcccttct cgagatgata ccgttcgacg atgcgcgcct
      661 ggtcgctgtg cgcgtaccgc tgatcgacct gctccacgag gttcgtcgtc tccacgacga
      721 gcgtgttccc ttcccagcgg ccgctcgaat ggccgttccg gcccggcagg cggtccgcga
      781 cggggacgat gcggtttccc aggtacacac ggcgaacctc gttgtgcgct tcgtaaacga
      841 tgatgatctg ttccggccgc tggtgaatct ccatgggata tccgccggac ccgagcatcg
      901 acccgggcat ccccgatccg aggcagaacc ctccgggggt gtcgcccgtc ggtgcgacca
      961 gcgcccgata tttttcgacc ttctgcttgg ccacgtccgt cataggcagc ccgggtccgg
     1021 cgccgcgtcc cgccccacgc ccccgggccg ctccaggctc gttgtaggtc gtccagacgc
     1081 ccgtaaagtc cggctgctga gccgacgcgg tcgagccgaa tccgaggaag atcgcgatcc
     1141 cgaccgccgc ccgaaatccc cgatgcatgg ttccctccac gaggctacaa ggattggcgt
     1201 tgaggggctg cttgtcaacc cactgtccgc gcaggctgaa gcctgcgctc tactcgtcaa
     1261 ttgcctgcgc tctactcgtc aattcttcgc ctgggcggcg tctctcaatc gatcgagacg
     1321 gtcgcgattc ttctcgttct cgcagaacga ctcgatgaga tcggtatcag ggagcaggag
     1381 ctgcttgaac ttgaacgtga acggccggct gtaaatcttg gggtcctcga cggtgatctc
     1441 aacctccatc gtcccgaagt ccaggcgatg aaagcgctcg gtcacgcgaa gggcgtcgct
     1501 gtgaggatgc ccgaacgcgt cgagccaccc gttgtccttg aacccagtga cttcgaccac
     1561 cagcgtatcg ccatcccacc gcccgatcga gtgcccggaa aaggcgggct cgggatcggc
     1621 tggcagcggc cgcccgtccg tgtagatctg acggaagtac gtcaggtcct cgt
//
LOCUS       DBNGSH010000083         3356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1024094, whole genome shotgun sequence.
ACCESSION   DBNGSH010000083 DBNGSH010000000
VERSION     DBNGSH010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1024094"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="AC16CY_01870"
     CDS             <1..1449
                     /locus_tag="AC16CY_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352550.1"
                     /translation="LINMGVSEALDINNRRDQNVLRDVSGRVCSNAVDATERDRCVQG
                     IIDEAARVTSRNREDVRISLTQIETLLRALAYIEGHKSLILISQSLAIRDHTELDTAI
                     RLAGPARTSINVLAVDLGRDDVTINERPPTPMEDRRIQLEGLEELAAASRGALYRVVN
                     TGEGIFDRLASELSAHYVLGVEQRPSDLKRDRHRVDVSLRRRDVTIRSGQAFVLSPTL
                     NPKRSVQETIQEALSSPMAQSGVPLRVSTFAQRDKGGKVRLSLSAQIGQPGTPPGEFT
                     VAYAIVSNENRLVASDGNRYKLAANAGSPNEPLHFATGTLLDPGLYSLRFAVVDAEGR
                     RGSVSRDLTVFKMEAEEFATSDLIIGNLQPAGQSMRPNVEPHVTADRLAAYLELYSKT
                     PETLAATTVAFEIASDEGSPSLTSQPAQIGDSPQPPGRVATGVINTSTLAPGRYLARA
                     TMARDGKTVGVLTRPFVLERGESASTPTPPAK"
     gene            complement(1634..2074)
                     /locus_tag="AC16CY_01875"
     CDS             complement(1634..2074)
                     /locus_tag="AC16CY_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352551.1"
                     /translation="MSSADAVLACVLSLLGRSADSMPPIQLVSQAPDHASELVEAYIR
                     PNDPRIYVVTSTAVFRDAQTAGARSRCGSNADKKLASILAHEEWHLRHGRDERGAYGA
                     QLKAMIRLGIPGDSPMYRGVQRSMLAVLDAKEQQPGGISATRQR"
     gene            complement(2370..>3356)
                     /gene="selA"
                     /locus_tag="AC16CY_01880"
     CDS             complement(2370..>3356)
                     /gene="selA"
                     /locus_tag="AC16CY_01880"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR3352552.1"
                     /translation="GAEAATVVNNNAAAVLLVLNTLALRKEVPTSRGELIEIGGSFRM
                     PDIMKRSGCRLREIGTTNRVHLRDYAEAIGPKTGLVMKVHTSNYEVVGFTSGVPEEKL
                     AALCHARGVPFVVDLGSGTLVDLKRYGLPHEPTPSEAIAHGADVVTFSGDKLLGGPQA
                     GIIVGRADLLAAIARNPLKRALRVDKLTIAALAAVLTIYADPDRLRERLPVIRLLTRP
                     LAEIRAAAERVRPAVAAALDGSAIVSVVACESQIGSGALPTRTIRSAGLAVRPAARAG
                     AGSALQRIAAAFRALPVPVIGRIQDDAFCLDVRCLDDEPAFVAQLAHLTWSH"
BASE COUNT          577 a         1130 c         1132 g          517 t
ORIGIN      
        1 ctcatcaaca tgggcgtctc cgaggccctg gacatcaaca accggcgcga tcagaacgtg
       61 ctccgggatg tgagcggccg cgtctgcagc aacgcggtgg acgcgacgga gcgcgatcga
      121 tgcgtccagg gcatcatcga cgaagccgcg cgtgtgacgt cgcgcaacag ggaagacgtg
      181 aggatctcgc tgacgcagat cgaaacactc ctgcgcgcgc tcgcctacat cgaaggccac
      241 aagtcgctca tcctgatttc ccagagcctc gcgatccgcg atcacaccga gctcgacacc
      301 gcaatccgcc ttgccggccc ggcgcgcacg tcgatcaacg tcctggcggt ggatctgggg
      361 cgggacgacg tcacgatcaa cgagcggccg ccgaccccga tggaggatcg gcgcattcag
      421 ctcgaggggc tggaggagct cgccgcggcc agtcgcggcg ctctgtaccg agtcgtgaac
      481 accggagaag gaatcttcga tcgcctcgca tcggagctgt cggctcatta cgtccttggc
      541 gtcgagcagc ggcccagcga tctgaagcgc gatcgccatc gggtggacgt ctcgctgcgc
      601 cggcgtgacg tcaccatccg atccgggcag gcattcgtcc tgtcgccgac gctgaatccg
      661 aagcgctcgg tccaggagac catccaggaa gcgctctcct cgccgatggc gcaatcagga
      721 gtgccccttc gcgtgtcgac cttcgctcag cgcgacaagg gaggcaaggt ccggctctcg
      781 ctgtcggcgc aaatcggcca gcctggcacg ccgcctggcg agttcaccgt cgcgtacgcc
      841 attgtcagca acgagaaccg gctcgtggca agcgacggga acaggtacaa gcttgccgcg
      901 aacgccggca gcccgaatga gccgctgcac ttcgcgacag ggacgctcct cgatcccggc
      961 ctgtattcac tgcggttcgc cgtcgtggac gccgaggggc gccgcggaag cgtgtcgcgc
     1021 gacctgaccg tcttcaagat ggaggccgaa gagttcgcca cgagcgacct gatcatcggg
     1081 aacctgcagc cggccgggca gagcatgcgg cccaacgtcg agccgcacgt tacggcggac
     1141 aggcttgccg cgtacctgga gttgtactcg aagacgcccg agacgctcgc cgcaaccaca
     1201 gtcgccttcg agatcgcgag cgacgaagga tcgccttccc taacgtcaca gccggcgcag
     1261 atcggggaca gcccgcagcc gccggggcgt gttgccaccg gcgtaatcaa cacgagcacg
     1321 ctggctcccg gacgctacct cgcgcgcgcg acgatggcgc gcgacggcaa gactgtcggc
     1381 gtgctgacgc gccccttcgt cctggagcgc ggggagagcg cgagcactcc cacgccgccg
     1441 gcgaaatgaa cgtgcttggt gcttggtcct cggtcctggg tccgtcgcgt ggtccttggt
     1501 ccgtccttgg tccaggagtc cacagtcctg cggtcccgac ggaccaagga cgaagaacgg
     1561 accaaggacg aagaacggac caaggacgaa gaacggacca agaaccaggg ccgaaggacc
     1621 aaggaccaaa gcgctagcgt tgccgcgtgg cgctgattcc gccgggttgc tgctccttcg
     1681 cgtcgagcac ggcgagcatc gagcgctgga ctccccggta catggggctg tcgcccggga
     1741 tgccgagacg aatcatcgct ttgagctgcg cgccgtaggc gccgcgttcg tccctgccgt
     1801 ggcggaggtg ccactcctcg tgagcgagga tgctggcgag cttcttgtcc gcgttcgacc
     1861 cgcagcgcga gcgcgcgccc gcggtctgcg cgtcgcgaaa gacggcagtg gacgtgacga
     1921 cgtagatcct ggggtcattg gggcggatgt acgcttcgac gagctccgac gcatgatcgg
     1981 gcgcctgact caccagctga atggggggca tactgtcggc gctgcgaccc agcagcgaca
     2041 gcacgcacgc gaggaccgcg tctgcgctcg acatggccag cctccagcgg caggctggcc
     2101 tcgcctatac ttcccagcag tccagcccgc agcccataac tgcgagttgc gatcaggggc
     2161 gccggtgcgt tgccgcgcgc tggcgtcccg ccctgtcaac ggtttcttct tcccactacg
     2221 tggtggccgc tgcggtggtt aacaggagcg cgtgcgttgc agtgctggtc ccaccgggga
     2281 aaccgtgagc gtgatgtctg ttagacgctc gtttcgcaaa aacggttcac gcgatcacga
     2341 gcgtcccgcg attgacacga actgatctat cagtgtgacc acgtgagatg cgcgagctgc
     2401 gcgacaaacg cgggttcgtc gtcgaggcag cgcacgtcga ggcagaacgc atcgtcttga
     2461 attcggccga tcaccgggac aggcagcgcg cggaacgcgg cagcgatccg ttgaagcgcg
     2521 ctgccggcgc cagccctggc ggccggccgc acggccaggc cggcgctccg gatcgtccgc
     2581 gtcggaagcg cgccgctgcc gatctgactc tcgcaggcga cgacgctgac gatcgcggag
     2641 ccatccaacg ccgcggccac ggcgggccgc acgcgctctg ccgcggcgcg gatctcggcg
     2701 agcggcctgg tcagcagccg gatcaccggg aggcgctcgc gaaggcgatc gggatcggcg
     2761 tagatggtaa gcacggcggc gagcgccgcg atcgtcagct tgtccacgcg cagggcccgc
     2821 ttgagtggat tgcgcgcgat ggccgccagc agatcggccc gcccgacgat gatgccggcc
     2881 tgaggcccgc cgagaagctt gtcgccgctg aaggtcacca catccgcgcc gtgggcgatc
     2941 gcctccgacg gcgtcggctc gtgcggcaga ccgtagcgct tcaggtccac gagggttccg
     3001 cttcccaggt cgacgacaaa cggcacgccg cgcgcgtggc acagggcggc gagcttctct
     3061 tccggcacgc ccgaggtgaa gccgacgacc tcgtagttgc tcgtgtgcac cttcatcacg
     3121 agcccggtct tcgggccgat cgcctcggca tagtcccgca gatgcacccg gttggtcgtg
     3181 ccgatctcgc gcaggcggca gccggatcgc ttcatgatgt cgggcatccg gaacgagccg
     3241 ccgatctcaa tcagctcacc gcgcgaggtc ggcacttcct tgcgtagcgc aagcgtattg
     3301 agcaccagca ggacggccgc cgcattgttg ttgaccacgg tggcagcttc cgcgcc
//
LOCUS       DBNGSH010000084         2803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2552594, whole genome shotgun sequence.
ACCESSION   DBNGSH010000084 DBNGSH010000000
VERSION     DBNGSH010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2552594"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="AC16CY_01885"
     CDS             complement(<1..334)
                     /locus_tag="AC16CY_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HIR3352553.1"
                     /translation="MNARVLGILVATIGAAWATSAQSPATSDASRALTGAVDIHVHSF
                     PDERPRSIHAIDVAKLAASRGMRAIVLKNHYESTAGTAYLVRTVVPNVEVFGGIALNL
                     TVGGINAAA"
     gene            complement(331..1326)
                     /gene="gmd"
                     /locus_tag="AC16CY_01890"
     CDS             complement(331..1326)
                     /gene="gmd"
                     /locus_tag="AC16CY_01890"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3352554.1"
                     /translation="MAKRAIITGITGQDGSYLAELLLERGYEVTGVVRRTSSPNLWRI
                     EHLLDRISLRPADLLDQLSILRLIQDVKPHEFYNLAAMSFVPASWDQPLLTGDYNSMG
                     VTRVLEAIRHVDTSIKIYQASSSEMYGRVREVPQTELTPFYPRSPYGVSKVFGHYITV
                     NYRESYDMFAVSGILFNHESPRRGLEFVTRKVSDGVARIKLGLADQLALGNLDACRDW
                     GFAGDYVHAMWLMLQQEKADDYVIATGKTHSVRQLVEAAFGHAGLDWQKHVRVDPAFL
                     RPAEVDHLIGDASKARRVLGWEPSVSFERLVAMMVDADLATLSGPGKVAPAWTPR"
     gene            complement(1344..2225)
                     /gene="rfbD"
                     /locus_tag="AC16CY_01895"
     CDS             complement(1344..2225)
                     /gene="rfbD"
                     /locus_tag="AC16CY_01895"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR3352555.1"
                     /translation="MSSGPITRVLVTGAAGQLGAAVVRAFADLDVTALTRAALDIADA
                     GAVSRAVSWAAPHLIVNCAAFNDVDGAEDRAIEALSVNAFAVRNLARAAEDAGAALVH
                     YGTDFVFDGSATVPYTEDARPSPRGTYAASKLLGEWFALEAPRAFVLRVESLFGSPAN
                     WTGRRGTLDGIVAGLEQGRDVRVFTDRVVSPGYVVDIAAATRHLATSGAAPGLYHCVN
                     SGHATWHDVAREAAAQLGIAKPALVPVTMDQVRLKAPRPRFCALSVHKLSAAGFHMPS
                     WQDGLRRWLAARGARAT"
     gene            2301..>2803
                     /locus_tag="AC16CY_01900"
     CDS             2301..>2803
                     /locus_tag="AC16CY_01900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3352556.1"
                     /translation="MERIDVAVIGAGVVGLASALAIARDGRSVCVLERERRPGLGTST
                     HNSQVIHAGIYYPAGSLKARLCVEGARRLYEFCARYQVPHRRTGKLIVAHDDREVDAL
                     DALRTRAIANGVQGMELVDERFIRAREPHVHAKAALFSPNTGVVEAEALIGTLARLCA
                     ERDVFLLP"
BASE COUNT          464 a          944 c          974 g          421 t
ORIGIN      
        1 cggctgcggc gttgattccg ccgacggtga ggttcagcgc gattccgccg aagacctcca
       61 cgttcgggac gacggtcctc accagatagg ccgtgcccgc cgtcgactcg tagtggttct
      121 tgaggacaat ggcccgcatg ccgcgcgagg cggcgagctt tgcgacgtca attgcatgaa
      181 tggagcgtgg gcgctcgtct ggaaagctgt ggacgtgaat gtcgacggcg cccgtgagcg
      241 cgcgcgacgc atccgacgtt gcagggcttt gagcggaggt cgcccatgca gcgccaatcg
      301 tcgccacgag gattccgagt acgcgtgcgt tcaccgggga gtccaggcgg gcgctacctt
      361 gcccgggccg gacagcgtcg caaggtcggc gtccaccatc atggccacga gacgttcaaa
      421 ggacacgctc ggctcccagc cgaggacgcg ccgcgccttg gacgcatcgc cgatcaggtg
      481 atcgacctcg gccgggcgca ggaacgccgg gtcgacgcgg acgtgcttct gccagtcgag
      541 gcccgcgtgc ccgaaggcgg cctccacgag ctggcgcacc gaatgggtct tgccggtggc
      601 gatgacgtag tcgtcggcct tctcctgctg cagcatcagc cacatggcgt gcacgtagtc
      661 gccggcaaag ccccagtccc ggcacgcatc gaggttgccg agcgccagct ggtcggccag
      721 cccgagcttg atgcgcgcga cgccgtcgct gactttccgc gtcacgaact cgagcccgcg
      781 ccgcggcgac tcgtggttga agagaatgcc cgacacggca aacatgtcgt agctctcgcg
      841 gtagttcacg gtgatgtagt gcccgaacac cttcgacacg ccgtaggggc tgcgagggta
      901 gaacggcgtc agctcggtct gcggaacctc gcggacccgc ccgtacatct cgctggacga
      961 ggcctggtag atcttgatcg aggtgtcgac gtgccgtatc gcctcgagca cccgcgtgac
     1021 acccatcgag ttgtagtcgc ccgtgagcag cggctggtcc cacgacgcgg gcacgaagga
     1081 catggcggcg aggttgtaga actcgtgagg cttgacgtcc tggatcaggc gcaggatcga
     1141 gagctggtcg agcagatcgg ccggccgcag cgagatgcga tcgagcagat gttcgatccg
     1201 ccagagattg ggcgagctgg tccggcggac gacgcccgtg acctcgtagc cccgctcgag
     1261 gagcagttcc gccaggtacg agccgtcctg accggtgatt ccggtgatga tcgcgcgctt
     1321 agccatgcag gttgtgcctc attctacgtc gcccgcgcgc cgcgcgcggc gagccagcgg
     1381 cgcaggccgt cctgccatga aggcatgtgg aaacctgcgg cagacaactt gtggaccgac
     1441 aacgcgcaga accgggggcg tggcgccttc agccggacct gatccatcgt caccggcacc
     1501 agcgccggct tcgcgatccc gagctgcgca gcggcctcgc gcgcgacgtc gtgccaggtg
     1561 gcatggccgg agttgacgca atggtacaaa ccgggcgcgg cgcccgacgt cgccaggtgc
     1621 ctcgtggctg cggcaatgtc gacgacgtat ccgggtgaca cgacgcgatc ggtgaagacg
     1681 cggacgtccc tgccctgctc gaggccggcc acgatcccgt cgagcgtgcc gcggcggccg
     1741 gtccagttgg ccggcgaccc gaacaagctc tcgacgcgca gcacgaaggc gcgcggcgcc
     1801 tcgagcgcga accattcgcc gagcagcttg gacgccgcat aggtcccgcg gggcgacggg
     1861 cgcgcgtctt ccgtgtaggg aacggtggca gagccgtcga acacgaagtc tgtgccgtag
     1921 tgcacgagcg cggcccccgc gtcctccgcg gcgcgcgcca ggttccggac ggcgaacgcg
     1981 ttcaccgaca gcgcctcgat cgcccggtcc tcggcgccgt ccacgtcgtt gaacgcggcg
     2041 caattcacga tgagatgggg agccgcccag gacaccgcgc gggagacggc gccggcgtcc
     2101 gcgatgtcga gggccgcccg cgtgagcgcg gtgacgtcga gatcggcgaa ggcccggacc
     2161 accgcggcgc ccagctgccc ggcggccccg gtgaccagca cgcgcgtgat cggaccgcta
     2221 gacactgggg tcagatcttg atttttgcac gagaggcgcc agtcaccgca ttggccgtgc
     2281 aaaaatcaag atctgacccc atggaacgta tcgacgttgc cgtcataggg gcgggcgtgg
     2341 tgggactcgc gtccgcgctc gccattgccc gcgacggacg aagcgtgtgc gtcctcgaac
     2401 gcgagagacg acccggcctc ggcacgagca cgcacaacag ccaggtcatt cacgccggca
     2461 tctactaccc cgccggtagc ctcaaagcca ggctctgcgt cgagggtgcg cgccggctgt
     2521 acgagttctg cgcccgttac caggttcccc accgtcggac cggcaagctg atcgtggcgc
     2581 acgacgatcg cgaggtcgac gcgctcgacg cgctccggac acgcgcgatc gccaacggcg
     2641 tgcaggggat ggagctcgtg gacgaacggt tcatacgcgc gcgcgagccg cacgtgcacg
     2701 caaaggcggc cctcttctct cccaacaccg gcgtcgtcga agccgaggcc ctcatcggca
     2761 cgctcgcgcg gctgtgcgcc gagcgggacg tgttcctgct gcc
//
LOCUS       DBNGSH010000085        24249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2382923, whole genome shotgun sequence.
ACCESSION   DBNGSH010000085 DBNGSH010000000
VERSION     DBNGSH010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 24249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24249
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2382923"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="AC16CY_01905"
     CDS             complement(<1..727)
                     /locus_tag="AC16CY_01905"
                     /inference="COORDINATES: protein motif:HMM:NF025086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR3352557.1"
                     /translation="MRRSIGALVLCLLVPIIVAAQGRRASSFRQVVDYDSNLTFTRLI
                     YGSGLGDFGFGGSAWSHDYPAADRNLSAIIDYITHVRLRLDGTNVLDLDDPEIFDNPV
                     LYVWEPGYWTIQPSEAVRLREYLLKGGFVIFDDFEGPDHWDNMVAQMRQALPEHRFIR
                     IDEHHPIFHSFYDIRKLDVPHPSMNVPPAYYGMFENNDPKGRMMALANHNNDIAEYWE
                     WSAEGLYNPDPTSNAYRLGVNYYV"
     gene            complement(766..1581)
                     /locus_tag="AC16CY_01910"
     CDS             complement(766..1581)
                     /locus_tag="AC16CY_01910"
                     /inference="COORDINATES: protein motif:HMM:NF025086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR3352558.1"
                     /translation="MRIRIALTAALILCITGAAAAQFGFGRSRYPPRYPKADTFGYGF
                     NFCRGIYTSTRGEAGGQGWSTDYPDADLNFSIRLSELTKTRVKFEGGGTPDFVTIRLT
                     DDALFQCPYLHMEDVGTVTFSPREVEALRAYLLKGGFIWVDDFWGSYAWDEWVAQIER
                     ILPRPGYVIQDLTPDHAIYQMQYQMTELPQIPSIQAWRGNSTDTSERGEDSAVPHFRA
                     ISDARNNIMVLMTHNTDISDAWEREGEDPRYFYQFSPRGYAVGINAVLYAMTH"
     gene            complement(1578..5156)
                     /locus_tag="AC16CY_01915"
     CDS             complement(1578..5156)
                     /locus_tag="AC16CY_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR3352559.1"
                     /translation="MLERLRRTPPTDLLGIVAEVRRRWRLKLIVRGALRVFGITVAVF
                     FAAAYLMEWARFSPLSIVASRVLIAVTLLASAYWFLFRPLRQRVTDEQVALYLEEHEP
                     SLQATLLSAVESSRTGHVPESAALVQRVVEQAIEVCARMDASHRVEQTPLQRNGVALG
                     TIVAAALLIVLGGPAFIRHSVTAVLLFSEGVEAASPYRIEVTPGDATVPKGVDQTISA
                     SLVGFGSEDVVLMVRRSPDAEFEPLPLLRNDEGRYEGIVFDIAAPLEYQVIADRVHSK
                     VHILKVVDVPYVQRLEMEYHYPAYTGLEPQTIEDGGDIATLRGTEVRLRIFPTMKTSG
                     GRITLNEKESVDLALQADGSLTAAFKVEREGSYRVELNAPAGERVIASPQYAIDVLAD
                     RAPSVSFNRPGRDTSVSAIEEVFVEAAAEDDFGVRDLELVYSINGGPEKVLKLFSGTK
                     RLPEVSAGHTFYLEELGVETGDSVSYYARASDNDVSGAKSASSNLYFLRIRPFKKDFR
                     QAPSQGGGGGGGGGGRGGQVQALSEQQRQIISATFNVQKDRKTLSAAKLRESSTVVSL
                     SQQRLREQVEGLLTRMNSQLVERDPAFAKIAELLPQAVAAMKDAEGKLSVAAPDQALP
                     PENKALQILQKAEEEYETQISVGRQQGGGGGGGGAIQQELAEIFEQELDKMANRYETA
                     NQASEQANDREVDELLEKLKELARRQEQEAERQRRRALEGMSSGGSSGAQQRALADQV
                     EEAARRLERLAREEQRPELQQAARELREAADAMRKAAAGGGANASAQAQAALERLRQT
                     ERQLQRSLTARAERDIEEAKREAEAIARQQQEIADRVRQLPAAPSARGDQARQINEKK
                     DQLEARLGKLEADLDEAARDATREERAASRKMAEAAGEIRDSRVRDSVLYSKGLVTRG
                     TNLQNANAAESEISEAIDNVRKRLDEAQAALGQGDPRDRREEALRRAQRLARAAESIQ
                     ERTRERALREQGQQGQRGQQGQQGQEGQQGQQGQQGQQGQQGQQGQQGQQGQQGQGGQ
                     GGGDRQARADGTRGGDGRFDPGGVDRGGVWRGGANWGGWWEGGRVRLTPEDIRQLSAE
                     ARQYTSEAQELRGMLRGENIDPRELDQIVEALRKLQDDRTYQNVEELARLQAFVADNL
                     KRFEFGLRRQVEADAGAIVLSGSDEVPKEFRKLVEEYYRSLGQAPR"
     gene            complement(5207..7234)
                     /locus_tag="AC16CY_01920"
     CDS             complement(5207..7234)
                     /locus_tag="AC16CY_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HIR3352560.1"
                     /translation="MQFLAPLFFVALAGLAIPVLLHLTQREKKQIVRFPSLMFVRRIP
                     YQSVRRRKIQHWLLLMVRLAALALIILAFTRPLVDRTDLPTAGSSAREVVILLDASYS
                     MGFGDRWARAQEAAREEIARLTASDRGSVVLFGSGAEIQIRSTAERSRLQASVDAAAP
                     GVSATRFAPALKVAGGIVADSTLPRREVVLISDFQRGGWRGEEGARLPPNTTLTPVLI
                     QGPAEQPNLSVAGVSMARSTSSNQQRVTVTAGIVNRTERPSTGGSVTLDVNGLSIGTK
                     PIAVDAGSSTSVTFDPFTVSSPNMRATVRVPDDALATDNTFNFVVSPSRPVRLTLVDR
                     GNPAAGLYLRRALSIGDAPKFEMVSRLPGEVTDDDLRLSAVVLLNDVEVSASLAKRLA
                     RYVDQGGGMFVATGPRATWPQEVDVLPATIGPPVDRSRGDAARVGGLEYGHPVFEPFR
                     APRSGDFSAIPVYGYRNLTAAKDARVLARFDAGTPAVLERRVGRGRILQWSSSLDVAW
                     SDFPTKPVFPIFVHQAARHLAGYAEPAPWLTVGQVLNASSVAGATKAPLRVLLTPSGR
                     RVPVEDEGTDVLELTEQGFYELRADRNEAEAVVAANVDPAESDLTPIDPKEITAAASG
                     GGGTGTAGATEGVPLTREAREKNQRLWWYFLVAGILLLGTDTLLSNRIAKA"
     gene            complement(7285..8199)
                     /locus_tag="AC16CY_01925"
     CDS             complement(7285..8199)
                     /locus_tag="AC16CY_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3352561.1"
                     /translation="MPVEVHAIPGARFMDPVVLARIGNLELVARAVVDGFINGLHKAP
                     YFGASVDFAEHRGYVPGDDIRRMDWRLWARTDRYYIKEYEAESNMNFSVLLDVSKSMD
                     YGSRGITKFDYARILTACLTNLVHHQRDRVGFVAFDHDVVEHVPPSAKHMETVLHVLE
                     RLTPGRPGRLKEPLHRLAEHFGRRGVLVVISDFYEEPESVVDAVSPLRFRGNDIVVFH
                     ILDPSELDFGFGDAQPFEDLESGEQMPVVPEAFRDEYRSLVKAHTDALTQAFSAVRVD
                     YTVLDTSKPLDFALFRYLNGRLKTLKIR"
     gene            complement(8201..9238)
                     /locus_tag="AC16CY_01930"
     CDS             complement(8201..9238)
                     /locus_tag="AC16CY_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3352562.1"
                     /translation="MNPMVSDAVDLQSADDIALADRMNAGRQQIVTELKKIIVGQDEV
                     IGQVLTTLFVGGNSLIVGAPGLAKTLLIHTIARVLDLKFSRIQFTPDLMPSDITGTDL
                     VQEDAVTGRRQMVFAPGPIFANIVLADEINRTPPKTQSALLEAMQEHRVTIQGRTYTL
                     DEPFYVFATQNPIELEGTYPLPEAQLDRFMFHVIIDHPPYDEELGVVRTTTALQNPQL
                     ERPVSGKDLVAFQELVRKVPVAEPVMRHALDIVRATRPRNPDTKDFISKWVAFGASVR
                     AAQYLVLGAKARALTTGRYHVSFDDIRALAHPVLRHRIIINFHAQSEGVTTDAIVDRL
                     LDAVPLPRSGM"
     gene            complement(9308..10228)
                     /locus_tag="AC16CY_01935"
     CDS             complement(9308..10228)
                     /locus_tag="AC16CY_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HIR3352563.1"
                     /translation="MLKVFLLIVVGLAGDPEHAKTFHGWGNSLAEASERFGVAPERLA
                     YLVDKPMEGDQRVTGVSNRESIVKALETIASAAGPDDLVFVTLIGHGAWDGRSAKFNI
                     PGPDVSAEEFQALVRKLPAKQVVFVNTASASGPFVEVLSGPGRTIVAATRSGAEHYTT
                     LFGGFFIDALSSPNADADKNKRISVLEAFQFAKAEVTRAFEREGLLVTEHALLDDDGD
                     KKGSPEPAATAADGKVAAVLSLADAGGGLPSDPRLRELYLERRELERRVDALRLLKEG
                     MNPARYSSELEKLVTAIALKTREIRAAEAK"
     gene            complement(10239..12539)
                     /locus_tag="AC16CY_01940"
     CDS             complement(10239..12539)
                     /locus_tag="AC16CY_01940"
                     /inference="COORDINATES: protein motif:HMM:NF018757.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HIR3352564.1"
                     /translation="MFDALFEALFSYRPVVFQQGEFRFDVTGASLTAGAIVAVIATTA
                     ALTYRRTRSEEGRKRDQVILTTLRVVALGLVLFCLFRPTLIVRAAVDQQNVVAVLLDD
                     SRSMQIWDVGGKPRGAFVRDQFEAKDGPIMKSLSDRFLVRTFRFSSTASRLASGSPLT
                     FAGAKTNLGAALDGVRDELAGLPVAAIVLVSDGADTGEQPLDDALLGLKAAKLPVFTV
                     GVGAERLSHDVQIDRVSTPRTVLKDASLLLDVVVRQTGYGGRSVTVDVEDEGRIVGSE
                     KVTLPVDGSPASVRVRAKAGESGPRLFKFRVTPLDDELVTQNNSRDALLDVRDTQERI
                     LYFEGEPRWEMKFIRRAVADDKNLEAAVLQRIADNKFKRFFGDDPDDPEELAGGFPKT
                     REELFKYRGLILGSIEAGAFSGDQLQMIADFVDRRGGGLLMLGGARSFGEGGYGGTPV
                     ADALPLLIDPRRRASDPSPLVRLKITPTRAGQAHSLTQIAQSEAASLARWAELPLVTS
                     VNEEMPVKPGGTVLLEGIDEAGRKRDVLAWQRYGRGKAIALTLQDTWQWQMHVTMSLE
                     DQTQEHYWRQMLRWLVEGVPNTVEIRMPDRVEPGDPVAIEASVVDGQFVELNDASVVA
                     QIAKPDGTTLSVPLQWTGERDGEYRGTFVSTEPGAYGVTVDATRSGGQAVGSGAAYVR
                     AGPSEAEFFDPTMHTAPLQRIAEETGGRFYTPEGARGLTEDIRYAGRGVTSIEERELW
                     NMPIILIALMGLVCAEWGYRRMVGLA"
     gene            complement(12586..13737)
                     /locus_tag="AC16CY_01945"
     CDS             complement(12586..13737)
                     /locus_tag="AC16CY_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HIR3352565.1"
                     /translation="MTDTIPEATAGGQARRVIFIDLGRFLALVFMLYGHTVAALLAPQ
                     YQRGTWFDIWTFQRGLTSALFLLLSGFAFSVATSRHWGSHQRFSFRFLKRVSRFAVFI
                     LLGYALHFPVDRFSQLPMAGDEVWRAFLAVDVLQLIGATFLVVQVLVLVTRSRRVFTA
                     AAFVLAALIPIASHPVWAMDWGRLAPPWLAAYMSPAAGSLFPVFPWASYVLFGAGLGQ
                     IYVGWGASRLSSYALVALLAPGAAMVLVASLLASMDGTPWSADPWGFMPIQVPLRMGP
                     CLILLSLIAYASERLTRLPRLFAVVGQETLLIYFVHLCIVFGSVWNTGLVGLLGASLG
                     PWATLAAVVLLLASMAALGWYWHWLKHSRPRVARWTMAGAAAVLVGRLL"
     gene            complement(13738..14574)
                     /locus_tag="AC16CY_01950"
     CDS             complement(13738..14574)
                     /locus_tag="AC16CY_01950"
                     /inference="COORDINATES: protein motif:HMM:NF025086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR3352566.1"
                     /translation="MSARTARPVALPLVLLVSLLASGAAFAQFGRGYFDRAPRPAPAL
                     FPDRNFVVCRLWYTSVRSEPSGGGWRTDYPYGEINLTIRFSELTRAPVSWSDGREPNH
                     YVVRITDDALFECPFTMASDVGTIGLNDDEVERLRLYLLKGGFLWVDDFWGPLAWEQW
                     THELAKVLPPSEYPIEDVPLSDPIFQSQYVVEKVPQITNIQFWRSTRGAVTSERGEDS
                     AVPHFRVVRDHHGRIIVVMTHNTDIADSWEREGEDPAFFYQFSPDGYAVGINVLLHAL
                     TH"
     gene            complement(14633..15067)
                     /locus_tag="AC16CY_01955"
     CDS             complement(14633..15067)
                     /locus_tag="AC16CY_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0000423 - mitophagy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HIR3352567.1"
                     /translation="MRKSYGVAAISAAFVLGFLTRGVVPGEGVLQAQSGRVFELRTYT
                     APDGKLGELNARFRNHTMRIFQKHGMTNVAYFTPQDAPLSQNTLVYLISHANRDAAKQ
                     SWAAFQKDAEWQKVAQESQVNGKIVASVVSQFLVPTDYSPMK"
     gene            complement(15087..15395)
                     /locus_tag="AC16CY_01960"
     CDS             complement(15087..15395)
                     /locus_tag="AC16CY_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HIR3352568.1"
                     /translation="MHYLLMYDVVEDYVARRAPYRRDHIAKAREAEARGELVLAGAFA
                     KPADGAVFLFRGESPQAAEDFARSDPYVLNGLVTRWWVREWTTVVGRDAEVPLPQNLS
                     "
     gene            15443..17494
                     /locus_tag="AC16CY_01965"
     CDS             15443..17494
                     /locus_tag="AC16CY_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl-peptidase 3 family protein"
                     /protein_id="HIR3352569.1"
                     /translation="MIKAVSSSRLLSCLLIPTVLATLACGSSPEQSAAPAAPAQPASD
                     SGRKYLLETIDDAAVVQIYADQFSALPLREKVLIWHLYQAALAGRDIFYDQKHRNALE
                     MRRVLEAIVTHAAGVDGPTLAEIQRYTKLFWINNGPYNNLTARKFVLKTTPQAFAAAA
                     QAASKAGATFPVQEGESLDALLSRLQPMFFDPEVDPIVTNKTPGPGKDILTASANNLY
                     SGVSTADLRGFTERYGLNSRLVKSKNGLVEEVYRINGRYGKEIARIVGHLEAAVPHAT
                     APMAAALRALIQWYRTGEDSDRVKYDIAWVQDKDSPVDTMNGFIEVYMDPRGIKGSWE
                     GLVFYVNREKTERIRKFANNAQWFEDRMPYDARYRKPDVKGIVANAIDVVIETGDSGP
                     VTPVGINLPNDQRIREQYGSKSVSLSNVRESSDNATPAAMRQEFSWTPEEVERATKFA
                     ALAAELNTDMHEVIGHASGRQAEGFKGSPQSVIKEHFSALEEGRADLIGLYFVADPKL
                     VELGIVPAADHDAIVRAEYEAYTRNAIVQLRRIREGTQIEEDHMRNRQMIIHWLMANT
                     KAIERRQRDGKTFYVMVDPKAFHEGVGRLLVEVQRIKSEGDAAAAAKLFDTYGIHFDA
                     KLRDEVVKRVDALKLPSYTGFVMPKLTPVTDASGTITDVRISYPMDLTAQMLEYAEST
                     K"
     gene            complement(17505..18557)
                     /locus_tag="AC16CY_01970"
     CDS             complement(17505..18557)
                     /locus_tag="AC16CY_01970"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HIR3352570.1"
                     /translation="MSCSAPEPSLPSTDMTPQPRWGLVIHTGAGNFTLESLGDRREPM
                     RAAMTAALEAGHEVLSGGGSSLDAVQAAIVILEDSPHFNAGKGAVFTHEGTHELDASI
                     MDGATRKAGAVAGVTRIKNPILLARLVMEKSPHVMLAGAGAEAFAADHAGVEFVENSY
                     FSTERARQALERAREEERQRVKKGASAQPAMERDAPSAYFGTVGAVALDRHGNLAAGT
                     STGGMTNKRSGRVGDSPIVGAGTYASNDSCAISATGQGEYFIRYTVAHDICARVQYTG
                     LGVQAAADTVVLEVLRAAGGEGGVIGLDRAGNVALSFNVTGMGRGYIGPEGTPLVMFS
                     REDELKTESSKLKAGK"
     gene            complement(18595..20559)
                     /locus_tag="AC16CY_01975"
     CDS             complement(18595..20559)
                     /locus_tag="AC16CY_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3352571.1"
                     /translation="MKRRLTAALVAVVAPFVTPALQGQQPQEPPAVFRSQVDATEVDV
                     LVTDQRGNLVTDLTAGDFELFEDGRPQKIVSFTLVDIPIERVERSLASPDVEPDIGTN
                     EDGEGRVYIIAIDDIVEVQALRTRRFLRRFIEQEFAPNDVAAIVYMGRGQSRNTQDFT
                     GNRRLLLAAADKVSGGFAYESVPSTAERSNLVRNRMKALRDLVEFAATLKGRRKALIL
                     LTSGLGLDVFEALDYTGGTKSIAFGDLHGAITAATRGNVAIYPIDPAGLTIGGDNLGE
                     GEFVAFSGLEATIDLRALAEATGGFAVVNTNSIQESFARIVRDNSRYYVLGFSSTNDR
                     RDGRFRKLDVRVKRPGLVVQARRGYLAPLGGRPQTAPLTAAEKGRIPPAIAESLGSPV
                     PNGAVPMRVFAASYQRGTQRAKVAIALEMNASALGFVEKDGKVATDVVVAVAAVSADG
                     KIHPGPRHEARVSFNKDATERTTAGGVRVLTEMQLPPGRYQLRAAGGTPTRAGSVMYD
                     LDVPDYTRTGLTMSGVALTADSARSGITVRPFDPLREELPGPITATREFQAGDRLSVY
                     AEVYEKLQSAAEHVIEMKVELRTDAGRVIATTREERSSKELAGAGGHGFLAQVPLEDA
                     APGLYVIHVEASANVGDRPTASRDILIRVL"
     gene            complement(20633..20974)
                     /locus_tag="AC16CY_01980"
     CDS             complement(20633..20974)
                     /locus_tag="AC16CY_01980"
                     /inference="COORDINATES: protein motif:HMM:NF049804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7662 domain-containing protein"
                     /protein_id="HIR3352572.1"
                     /translation="MWKRTARLASIAPDKSSPEATATEPRPRVVSGDCAPLHRYLEHR
                     YADVVVLTFGQIEDLLGFALPQGARIDRAWWTPRLTPADGRPFSDAWTLAGRTATPNL
                     LAKNVVFERKR"
     gene            complement(21435..23066)
                     /locus_tag="AC16CY_01985"
     CDS             complement(21435..23066)
                     /locus_tag="AC16CY_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3352573.1"
                     /translation="MTVHLVNPSHLSFGVGVITPRWLFVLGAATPPEYGRPRIVDETL
                     EPLDLDTIVAGDIVGIGIHTGNALRGYELGAAARARGATVVFGGIHATLYPDEAYERG
                     GAHTVVRGDGDVVWPLVIDHAVQGTLQPRYDGGRVDADQFVPARWDLLPAGRYMWGSV
                     QTVRGCPKHCSFCSVWRTDGQKPRQRGVDSVVAEIVELRRRGFRFVALADDNFYPVTL
                     ADLAMAERQGNVARLEQLRGLRAERFELMARLAELPPDMVFFTQITMEAAEDDSFLDA
                     MRCANIKGALVGVEAVTPEGLKDIYKNFNDVGDALVQRLRKFREHGVYVLGSFIFGLP
                     SDRPSTFDATADVAERAELAFAQFVMLTPYPGTVDFEAWEKKVGAEAQKVAGIPITRH
                     WLIPQAHRPKVYTPHPVMTPDEIRARTQAVWDRFYSLRRVWERSRCTPTLRARLAFVL
                     LSKLYRQMYANTGIATDSARVNRANRWARLIARPCRLLFTAKPLPGLTSESDVPLRPP
                     RSNPVLRGGLALQALEFKSERTRSASVPGGRGERG"
     gene            23379..23567
                     /locus_tag="AC16CY_01990"
     CDS             23379..23567
                     /locus_tag="AC16CY_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352574.1"
                     /translation="MDPAEAQARVIIAAALIVRGAVEVPAMPSGSQYVPDPAGVRLRQ
                     LTDYVYRLLTIDEPQEAR"
     gene            23694..23903
                     /locus_tag="AC16CY_01995"
     CDS             23694..23903
                     /locus_tag="AC16CY_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352575.1"
                     /translation="MAIDVSTVAAPPQAAISTPAPESVGFDERWAAWQAKGAAHDRAV
                     RRKIAVAAPILIVSAAVVFYVLLGR"
BASE COUNT         4062 a         8228 c         7982 g         3977 t
ORIGIN      
        1 agacgtaata gttcacgccc agccgatacg cgttgctggt cggatcgggg ttgtagagcc
       61 cttcggccga ccattcccag tactccgcga tgtcgttgtt gtggttggcc aacgccatca
      121 tgcggccctt tggatcgttg ttctcgaaca tcccgtaata ggcgggtggg acgttcatgc
      181 tgggatgcgg cacgtcgagc ttccggatgt cgtagaagga gtgaaaaatc gggtggtgct
      241 cgtcgatgcg gatgaagcgg tgctcgggca gcgcctggcg catctgcgcc accatattgt
      301 cccaatggtc cggtccctcg aagtcgtcga agatgacgaa gccccccttg agcaggtatt
      361 cgcgcaagcg gaccgcctcg ctcggctgga tcgtccagta tccgggctcc cagacgtaga
      421 gaacagggtt gtcgaagatc tccgggtcgt ccagatcgag cacgttggtg ccgtccagcc
      481 tgaggcggac gtgggtgatg tagtcgatga tggcgctcag gtttctgtcg gccgccgggt
      541 agtcgtgcga ccatgccgat ccgccgaagc cgaagtcacc gagccccgag ccgtagatca
      601 ggcgggtgaa cgtgaggttg ctgtcgtagt ccacgacctg ccggaacgac gaggcgcgcc
      661 ggccctgggc ggcgacgatg atcggaacga gcaggcagag gacgagcgcg cctattgacc
      721 gtcgcaccct cgcaccctcg caccttcgca ccttcgcacc ctgtctcagt gtgtcatcgc
      781 gtacagaacg gcgttgatcc ccacggcgta gccgcgcggc gagaactggt agaagtagcg
      841 cgggtcctcg ccttcgcgct cccaggcatc ggagatgtcc gtgttgtgcg tcatcagcac
      901 catgatgttg ttgcgagcgt cggaaatggc tcgaaagtgc ggcaccgcgc tgtcctcgcc
      961 gcgctcggac gtgtcggtcg agttgcctcg ccacgcctgg atcgacggga tctgcgggag
     1021 ctccgtcatc tggtattgca tctggtaaat ggcgtggtcc ggcgtcagat cctgaatcac
     1081 atagccgggc ctgggcagga tgcgttcgat ttgagcgacc cactcgtccc acgcatacga
     1141 gccccagaag tcgtccaccc agatgaagcc gcccttgagc aggtaggccc gcagggcctc
     1201 cacttccctg gggctgaagg tcacggttcc cacgtcctcc atgtgaaggt agggacactg
     1261 gaagagggca tcgtcggtca agcggatggt gacgaagtcc ggagtgccgc cgccttcgaa
     1321 cttgacgcgc gttttcgtca actcggagag gcggatcgag aaattcaggt cggcgtcggg
     1381 gtagtcggtt gaccagccct gcccgcctgc ctcaccgcgc gtgctcgtgt agatgccccg
     1441 gcagaagttg aagccgtagc cgaaggtgtc ggccttcggg tagcgcggag ggtagcggct
     1501 gcggccgaac ccgaactgcg ctgccgccgc gcctgtgatg cagaggatca gggcggcggt
     1561 caacgcgatc cggattctca ccgtggcgcc tgcccgagcg agcggtagta ctcctcgacg
     1621 agcttgcgga actccttcgg gacctcgtca gagcccgaca gcacgatggc gcccgcgtcc
     1681 gcctccacct gccgccgcag gccgaactcg aagcgcttga ggttgtcggc gacaaacgcc
     1741 tgcagcctcg ccagctcctc gacgttctgg taggtgcgat cgtcctgcag cttccgcagt
     1801 gcctcgacga tctggtcgag ctcccgcgga tcgatgttct cgccacgcag catgccgcga
     1861 agctcctgcg cctcggacgt gtactggcgc gcttcggccg agagctgccg gatgtcctca
     1921 ggcgtcaacc gaacacgacc gccctcccac cagccgcccc agttcgcgcc gcctcgccag
     1981 acaccgccgc gatcgacccc gccgggatcg aaacggccgt cgccgccgcg ggttccgtcg
     2041 gcgcgagcct gacgatctcc gcctccctgc ccgccctggc cctgctggcc ctgctggcct
     2101 tgctggccct gttgaccctg ctggccctgt tgaccctgct ggccttgctg accctgctgg
     2161 ccctcttgcc cttgttgacc ctgctggcct cgctgacctt gctggccctg ctcgcgcagc
     2221 gcgcgctcgc gtgtccgctc ctgtatcgac tccgcggcac gcgcgagacg ctgcgcccgt
     2281 ctcagggctt cttcgcggcg atcgcgcgga tcgccctggc cgagcgcggc ctgagcctcg
     2341 tccaggcgct tgcgcacgtt gtcgatggct tcggagatct cgctctcggc cgcgttcgcg
     2401 ttctggaggt tggttccacg cgtgaccagc cctttcgaat acagcacgct gtcacgcacg
     2461 cggctgtcgc gaatctcgcc ggcagcctcg gccatcttcc gcgacgcggc gcgctcctcc
     2521 cgtgtcgcgt cccgggcagc ctcgtcgagg tcggcttcca gcttgccgag ccgcgcctcg
     2581 agttgatctt tcttctcgtt gatctgccgc gcctggtcgc cgcgagccga cggcgccgcg
     2641 ggaagctgcc ggacccggtc ggcgatctcc tgttgctgcc gggcgatggc ctcggcctcg
     2701 cgcttcgcct cttcgatgtc gcgctcggcc cgcgccgtca gcgaccgctg aagctgacgc
     2761 tcggtctggc gaaggcgctc gagcgcggcc tgggcctgcg cggacgcgtt ggcgccgccg
     2821 cctgcggctg ccttgcgcat cgcgtcggcg gcctcacgga gctcacgcgc ggcttgctgc
     2881 agttccggcc gctgttcctc ccgcgcgaga cgctcgagcc gccgggccgc ttcctcgacc
     2941 tgatcggcga gcgcccgctg ctgggcgccg ctgctgccgc ccgagctcat accctcgagt
     3001 gccctgcgcc gctgacgctc ggcctcctgc tcctgccggc gggccagctc cttcagcttc
     3061 tccagcagct cgtccacttc acgatcgttg gcctgctcgg acgcctggtt ggccgtctcg
     3121 tagcggttgg ccatcttgtc gagctcctgc tcgaagatct ccgccagctc ctgctgaatc
     3181 gcgccgccgc ccccgccgcc gccgccctgc tggcggccga cgctgatctg ggtctcgtat
     3241 tcttcttcgg ccttctgaag aatctggagc gccttgttct cgggcggcag ggcctgatcg
     3301 ggcgccgcca cgctcagctt gccctcggcg tccttcatgg cggccacggc ctgcggcagc
     3361 agctcggcga tcttggcgaa ggccggatcg cgttccacca gctggctgtt catgcgcgtc
     3421 agcagccctt cgacctgctc gcgaagccgc tgctgcgaca gcgagacgac cgtcgaactc
     3481 tcgcgcagct tcgcggcgct gagcgtcttt ctgtccttct gcacgttgaa cgtcgcggag
     3541 atgatctgac gctgctgctc ggacagggcc tggacctgcc cgcctcgtcc gccgccgcca
     3601 ccgccgccgc caccgccctg cgacggcgcc tgacggaagt ccttcttgaa cggacgaatg
     3661 cgcaggaagt acaggttgct cgacgcggat ttcgcgccgc tgacgtcgtt gtccgaggcg
     3721 cgcgcgtagt acgagaccga atcgcccgtc tccacgccga gctcctcgag atagaacgtg
     3781 tggccggccg acacctcagg caggcgcttg gtgcccgaga agagcttcag caccttctcg
     3841 gggccgccgt tgatcgagta gaccagctcg agatcgcgga cgccgaagtc gtcctcggcg
     3901 gcggcctcga cgaatacttc ttcgatcgcg gacacggacg tgtcgcggcc cggacgattg
     3961 aaggagaccg acggcgcccg atcggcgagc acgtcgatgg cgtactgcgg cgaggcaatc
     4021 acgcgctccc ccgccggcgc gttgagctcc acgcggtacg agccctcgcg ctccaccttg
     4081 aaggccgcgg tcagcgaccc gtccgcctgc agcgccagat cgaccgactc tttctcgttc
     4141 agcgtgatcc gtccgcccga ggtcttcatc gtcgggaaga tgcgaagccg gacctcggtg
     4201 ccgcgcagcg tcgcgatgtc gccgccgtct tcgatcgtct gcggttcgag gccggtgtag
     4261 gccgggtagt ggtactccat ctccagccgc tggacgtacg gaacgtcaac gaccttcagg
     4321 atgtggacct ttgagtgcac ccggtcggcg atgacctggt actcgagcgg agccgcgatg
     4381 tcgaacacga tcccttcgta gcggccttcg tcgttgcgga gcagcgggag cggctcgaac
     4441 tccgcgtccg gcgatcgccg caccatcagg acgacgtcct cggagccgaa cccgacgagc
     4501 gacgccgaga tcgtctggtc cacgcctttc ggcacggtcg cgtcgcctgg tgtcacctcg
     4561 atgcgatacg gcgatgcggc ctccacgcct tcgctgaaca ggagcacggc ggtgaccgaa
     4621 tggcggatga aggccggccc ccccaggacg atcaacagtg cggcggcgac gatcgtgccc
     4681 aacgccacac cgttgcgctg aagcggagtc tgctcgacgc gatgcgacgc gtccatcctc
     4741 gcgcacacct cgatcgcctg ctcgaccacg cgctgcacca gggcagccga ttcgggaaca
     4801 tgacccgtgc ggctcgattc gaccgcgctg agcagtgtcg cctgaaggga cggctcgtgc
     4861 tcttccagat agagcgcgac ctgctcgtcg gtgacgcgct gccgcagggg gcggaagagg
     4921 aaccagtagg ccgacgccag gagcgtcacg gcaatcagca cccgcgaggc cacgatcgag
     4981 agcgggctga acctggccca ctccatcagg taggcggcgg cgaagaacac ggccaccgtg
     5041 atgccgaaga cgcggagcgc gcctcgaacg atcagcttca ggcgccaacg tcggcggact
     5101 tcggcaacga tgccgagcaa atcagtcggt ggagtgcgtc tcaggcgctc gagcatgggt
     5161 cctcactttt tacctaccgg ccgccagcta ccagctaccg gctagtctac gccttcgcaa
     5221 tacggttgga taggagcgtg tcggtgccca gcagcaggat tcccgcaacc aggaaatacc
     5281 accacaatct ctggtttttc tcgcgcgcct cgcgcgtcag cggtacgcct tcggtcgcgc
     5341 cggcggtccc cgtgccaccc ccgcccgagg cagccgccgt gatttccttc gggtcgatcg
     5401 gcgtcagatc cgactcggcg ggatcgacgt tggcggcgac gacggcttca gcctcgttgc
     5461 ggtcggcacg cagctcgtag aagccctgct cggtgagttc gagcacgtcg gtgccctcgt
     5521 cctcgaccgg aacgcggcgg cccgacggcg tcagcaggac ccgcagcggc gccttcgtgg
     5581 ccccagccac agacgaggcg ttgagcacct gcccgacggt gagccacggc gccggctccg
     5641 cgtagccggc gaggtgtctg gcggcctggt gcacgaatat cggaaagacc ggcttggtcg
     5701 gaaagtcgct ccaggccacg tcgagcgacg acgaccactg gaggatgcgt ccgcgcccca
     5761 cccgccgttc gagcacggcc ggcgtgccgg cgtcgaaccg cgcgaggacc cgcgcgtctt
     5821 tcgcggccgt gaggttgcga tacccgtaca cggggatcgc cgagaagtcg ccgctccgtg
     5881 gcgcgcgaaa cggctcgaac accggatgcc cgtactccag gcccccgacg cgggccgcgt
     5941 cgccccgcga gcgatcgacc ggcggaccga tcgtggccgg cagcacgtcc acctcctgcg
     6001 gccacgtcgc gcggggcccg gttgcgacga acattccgcc tccctggtcc acgtagcgcg
     6061 cgagccgctt cgccagcgac gcgctcacct cgacgtcgtt caataggacg acggcactca
     6121 gccgaaggtc gtcgtcggtg acctcgcccg gcaggcggga gaccatctcg aacttgggcg
     6181 catccccgat cgagagcgcg cggcgcaggt acaggcctgc ggccgggttg ccgcgatcca
     6241 cgagcgtcag ccgcacgggc ctggagggcg agacgacgaa gttgaacgtg ttgtcggtcg
     6301 ccagagcgtc gtccggcacg cgaacggtcg cgcgcatgtt gggcgagctc acggtgaacg
     6361 gatcgaaggt caccgacgtg ctactgccgg cgtccacggc gatcggcttt gtgccaatcg
     6421 acaacccgtt cacgtcgagc gtgacgctgc cgccggtcga aggccgttca gtgcggttca
     6481 cgataccggc cgtgacagtg acccgctgct ggttggagga ggtcgagcgc gccatcgaga
     6541 cgccggcaac gctcaggttc ggctgctcgg ccgggccctg aatcaaaacc ggtgtcagcg
     6601 tcgtgttcgg tggcagcctc gctccttctt ctccgcgcca gccgcctcgc tgaaagtcgc
     6661 tgatgagcac gacctcgcgg cgcggcagcg tggagtctgc gacgatacct ccagccacct
     6721 tgagcgcggg ggcgaaccgc gtcgcggaca cgccgggcgc ggccgcgtcc accgacgcct
     6781 ggaggcgcga acgctccgcg gtggaccgga tctggatctc ggctccggac ccgaagagca
     6841 cgaccgatcc ccgatccgac gcggtgaggc gcgcgatctc ctcgcgcgcc gcctcctggg
     6901 cgcgggccca gcgatcgccg aaccccatgc tgtagctcgc gtcgagcagg atcacgactt
     6961 cgcgcgcgga cgaaccggcc gtcggaagat ccgtgcgatc gacgagggga cgcgtgaagg
     7021 ccaggatgat cagggcgagc gccgccaggc gcaccatcag gaggagccag tgctggatct
     7081 tgcggcggcg gacggactga tacggaatcc gccgcacgaa catgagcgac ggaaagcgga
     7141 cgatctgctt cttctcgcgc tgcgtgaggt gaagcagcac gggtatcgcc aggccggcga
     7201 gcgcgacaaa gaagagcggc gcgagaaact gcataggcta cagtgcaacg gtacgacggt
     7261 gcgacggtgc gagggtgcct gtcttcatcg gatcttcagg gtcttcaggc gcccgttcaa
     7321 gtaccggaac agcgcgaaat ccaggggctt cgacgtgtcg agcacggtgt aatccacgcg
     7381 gacggccgag aacgcctgcg tcaacgcgtc ggtatgcgcc ttcacgagcg accggtattc
     7441 gtcgcggaag gcctccggaa cgaccggcat ctgctcgccg ctctccaggt cctcgaacgg
     7501 ctgagcgtcg ccgaagccga agtcgagctc cgacggatcg agaatgtgaa agacgacaat
     7561 gtcgttcccg cgaaagcgga gcggcgacac cgcgtccacg accgactccg gctcctcgta
     7621 gaaatcggag atcacgacga gcacgccgcg cctgccgaag tgctcggcca gcctgtgcag
     7681 cggctccttg agccggccgg gccggccggg cgtcaggcgc tcgagcacgt gcagaaccgt
     7741 ctccatgtgc ttggcggagg gcggcacgtg ctccacgacg tcgtgatcga aggcgacgaa
     7801 gccgacgcgg tcgcgctgat ggtgcaccag gttggtgagg caggccgtca ggatccgcgc
     7861 gtagtcgaac ttcgtgatgc cgcggctgcc gtagtccatc gacttcgaca cgtcgagcag
     7921 cacggagaag ttcatgttcg actcggcctc gtactccttg atgtagtacc ggtcggtgcg
     7981 ggcccagagg cgccagtcca tgcggcggat gtcgtcgcca gggacgtacc cgcggtgctc
     8041 ggcgaagtcc accgaggcgc cgaagtacgg cgccttgtgc aggccgttga tgaacccgtc
     8101 caccacggcg cgggcaacga gctcgaggtt cccgatgcgc gcaagcacaa cgggatccat
     8161 gaagcgcgcg ccggggatgg cgtggacttc taccggcatc tcacattccc gatcgaggca
     8221 gcggcacggc gtcgagcagg cgatcgacga tcgcgtcggt cgtcacgcct tctgactgcg
     8281 cgtggaagtt gatgatgatg cggtgccgca gcaccgggtg cgcgagcgcc cggatgtcgt
     8341 cgaagctgac gtggtaccgc cccgtcgtga gcgcgcgcgc cttcgcgccg agcaccaggt
     8401 actgggccgc ccggacgctg gcgccaaacg ccacccactt ggagatgaag tccttggtgt
     8461 ccggattcct cggacgcgtc gcgcggacga tgtcgagcgc gtgccgcatc acgggctcgg
     8521 ccacgggcac ctttcgcacg agctcctgaa acgcgaccag gtccttgccg ctgaccgggc
     8581 gctcgagctg cgggttctgc agcgccgtgg tcgtcctcac cacacccagc tcttcgtcgt
     8641 acggcggatg gtcgatgatg acgtggaaca tgaaccggtc gagctgcgcc tcgggcagcg
     8701 gataggtgcc ttcgagctcg atcgggttct gcgtcgcgaa cacgtagaac ggctcgtcca
     8761 gagtgtaggt ccgtccctgg atcgtgaccc ggtgctcctg catcgcctcg agcagagccg
     8821 actgcgtctt gggcggtgtg cggttgatct cgtccgccag cacgatgttg gcgaagatcg
     8881 ggccgggcgc aaacaccatc tgtcgccggc cggttaccgc gtcttcctgc acgaggtcgg
     8941 tgccggtgat gtcggagggc atcaggtcgg gcgtgaactg gatgcgcgag aacttcaggt
     9001 ccaggacgcg cgcgatcgtg tggatcagca gcgtcttggc cagacccggc gcgccgacga
     9061 tcaggctgtt gccgcccacg aacagcgtgg tgagcacctg cccgatcacc tcgtcctggc
     9121 cgacgatgat tttcttcagc tccgtgacga tctgctggcg cccggcattc atccggtccg
     9181 cgagcgcgat gtcgtccgca gactgcaagt caaccgcatc actcaccatc gggttcatcg
     9241 ggtccctcca aaatggggtc gggagtcatt tttcgaggac actcttgaaa aatgactccc
     9301 gaccccatta tttcgcttcc gccgcgcgaa tctcgcgcgt cttcaaggct atcgccgtca
     9361 cgagcttctc gagctcggag ctgtagcggg ccggattcat cccctctttc agcagccgga
     9421 gcgcgtccac acgtcgctcg agctcgcgcc gttccaggta cagctcccgc agcctcgggt
     9481 ccgagggcag gccgccgccg gcgtcggcga gcgacagcac cgccgccacc ttgccgtccg
     9541 ccgccgtggc agcgggctcc ggactcccct tcttgtcccc gtcgtcgtcg agcagcgcgt
     9601 gctcggtgac gagcaggcct tcgcgctcga acgcgcgcgt cacttcggcc ttggcgaact
     9661 gaaacgcctc gagcacgctg attcgcttgt tcttgtccgc gtccgcgttg ggcgagctca
     9721 gggcgtcgat gaagaagccg ccaaagagcg tcgtgtagtg ctccgcgccg ctgcgcgtgg
     9781 cggcgacgat cgtccggccg ggaccggaga gcacctcgac gaacggaccg ctggcgctcg
     9841 ccgtgttgac gaacacgacc tgcttggccg gcagcttgcg gacgagcgcc tggaactcct
     9901 cggcgctcac gtccgggccc ggtatgttga acttcgccga tcgcccgtcc caggccccgt
     9961 ggccaatcag cgtgacgaag acgagatcgt ccggcccggc ggcggacgcg atcgtttcaa
    10021 gcgccttgac gatgctctcg cggttcgaga cgccggtgac gcgctggtcg ccctccatcg
    10081 gcttgtccac gaggtaggcg aggcgctccg gcgccacgcc gaagcgctcc gacgcttcgg
    10141 cgagcgagtt gccccacccg tgaaacgtct tggcgtgctc ggggtcaccg gccagcccga
    10201 cgacgatgag caggaagacc ttcagcatcc ctcgcgtctc acgccagtcc gaccatacgc
    10261 cggtagcccc actcggcgca taccagaccc atgagcgcga tgaggatgat tggcatgttc
    10321 cagagttcgc gttcttcgat tgacgtgacg ccgcgtccgg cgtaccggat gtcctcggtc
    10381 agcccgcgag ccccctcagg cgtgtagaag cggccgcccg tctcctccgc gatccgctgc
    10441 agaggcgccg tatgcatcgt cggatcgaag aattcggcct cgcttggacc cgcgcgcacg
    10501 tacgccgctc cggagccgac ggcctgcccg cccgatcggg tggcgtcaac ggtgaccccg
    10561 taggcaccgg gttcggtgct cacgaaagtg ccgcggtact cgccgtcgcg ctcgcccgtc
    10621 cattggagcg ggaccgacaa tgtggtgccg tcgggcttgg caatctgcgc aacgacgctc
    10681 gcgtcgttca actcgacgaa ctgcccgtcc acgaccgagg cttcaatcgc caccggatcc
    10741 ccgggctcga cgcgatccgg catgcgaatc tccacggtgt tgggaacgcc ctcgaccagc
    10801 caccgcagca tctgacgcca gtagtgctcc tgcgtctgat cctcgagcga catcgtgacg
    10861 tgcatctgcc actgccacgt gtcctgcagc gtcagcgcga tcgccttgcc gcggccgtac
    10921 cgctgccagg cgagcacgtc acgcttacgc ccggcctcgt cgatcccctc gagcaggacg
    10981 gtgccaccgg gcttcacggg catctcctcg ttgaccgagg tgacgagcgg cagctcggcc
    11041 catcgagcca acgacgccgc ttcggactgc gcgatctgtg tcagggagtg cgcctgaccg
    11101 gcgcgggtcg gggtgatctt gagtcgcacc agaggcgacg gatccgaggc ccggcgccgg
    11161 ggatcgatga ggagcgggag cgcgtcggcc acgggcgtgc ccccgtagcc cccttcgccg
    11221 aacgaccgcg cgccgccgag catcagcagg ccgccgcccc gtcgatcgac gaagtcggcg
    11281 atcatctgga gctggtcgcc cgaaaacgcg cccgcctcga tgctgccgag gatcagaccg
    11341 cgatacttga acagctcctc gcgcgtcttc gggaaacctc cggcgagctc ctccgggtcg
    11401 tccgggtcgt cgccgaagaa gcgcttgaac ttgttgtcgg cgatgcgctg cagcaccgcg
    11461 gcttccaggt tcttgtcgtc ggcaacagcg cgcctgatga acttcatctc ccagcgcggc
    11521 tcgccttcga agtacaggat cctttcctgc gtatcccgca cgtcgagcag ggcgtcccgc
    11581 gagttgttct gcgtgacgag ttcgtcgtcg agcggagtca cccggaactt gaacagccgg
    11641 ggcccggatt cgccggcctt ggcgcgcacg cgaaccgacg cgggcgagcc gtcgacgggc
    11701 agcgtcacct tctccgatcc gacgatgcgg ccctcgtctt ccacgtccac ggtgaccgaa
    11761 cggccgccgt atccagtctg ccgcacgacg acgtcgagca gcagcgacgc atccttcagg
    11821 acggtcctcg gcgtgctcac gcggtcgatc tgaacatcgt gggagagccg ctcggccccg
    11881 acgcccaccg tgaacacggg cagcttggcg gccttgagcc cgagcagcgc gtcgtcgagc
    11941 ggctgctcgc ccgtgtcggc tccgtcggag acgagcacga tggccgcaac gggaaggccc
    12001 gcgagctcgt cccgcacgcc atcgagcgcg gcgcccaggt tggtcttcgc gccggcgaag
    12061 gtcagcgggc taccggatgc cagccgcgag gcggtcgacg aaaaccggaa ggtcctgacc
    12121 agaaaccggt cggacagcga cttcatgatg ggaccgtcct tggcctcgaa ctgatcccgg
    12181 acgaaggcgc cgcggggctt gccgccgaca tcccagatct gcatgctgcg ggaatcgtcg
    12241 agcagcaccg ccacgacgtt ctgctgatcc acggccgcgc gcacgatcaa cgtggggcgg
    12301 aacaggcaga acagcacgag gcccagggcg acgacgcgaa gcgtcgtcag aatcacctgg
    12361 tcgcgcttcc taccctcctc tgagcgggta cggcggtagg tcagggccgc ggtggtcgcg
    12421 atgacggcca cgatcgcgcc ggcggtgagg gacgcacccg tgacgtcaaa gcggaactca
    12481 ccttgctgaa agacgaccgg ccgataggag aacaaagctt cgaacagggc gtcgaacatc
    12541 aggggcctcg agactcagga gcgccgaatt gtaccccact gggggtcaca gcaggcggcc
    12601 caccaggacc gccgccgcgc ccgccatcgt ccaccgggcc acacgcggcc gggaatgctt
    12661 cagccaatgc cagtaccatc ctaacgccgc catcgatgcc agcagcagga caaccgcggc
    12721 cagggtcgcc cagggcccga gcgacgcgcc gagcagcccg accagcccgg tgttccacac
    12781 tgagccaaag acgatgcaca ggtggacgaa gtagatgagc agggtttcct ggccgacgac
    12841 agcaaaaagg cgcggcagcc gggtcagccg ctcgctcgca tacgcgatca gggacagcag
    12901 gatgaggcat ggacccattc gcagcgggac ctggattggc atgaaacccc atgggtcggc
    12961 gctccaggga gtgccgtcca tggacgcgag aagcgacgcg acgagaacca tcgcggcgcc
    13021 gggcgccagc aaggcgacga gcgcgtagct cgacagacgt gacgcgcccc agccgacgta
    13081 gatctgtccc aggcccgcgc cgaacaggac gtaggaggcc cagggaaata cgggaaacag
    13141 cgagccggcg gcgggcgaca tgtacgcggc gagccacggc ggcgccagcc ggccccagtc
    13201 catcgcccag acgggatgag acgcgatggg aatgagcgcc gcgagcacga acgccgccgc
    13261 ggtgaacacg cgcctcgaac gggtgacgag cacgagcacc tgcacgacca ggaacgtcgc
    13321 gccgatcaac tgcagcacgt ccaccgcgag aaacgcgcgc cacacctcgt cgcccgccat
    13381 ggggagttgg ctgaaccggt ccaccgggaa gtgcaacgcg tagccgagca ggatgaagac
    13441 cgcgaaccgg ctgacccgtt tcaagaacct gaacgaaaag cgctggtgcg acccccagtg
    13501 acggctggtg gcgacgctga acgcgaagcc ggacagcagc aggaagagcg ccgacgtcag
    13561 cccccgctgg aacgtccaga tgtcgaacca ggtgccgcgc tgatactgcg gcgccaggag
    13621 cgcggcgacg gtgtgtccgt atagcatgaa gaccagtgcc aggaagcggc ccaggtcgat
    13681 gaagatgacg cggcgggcct gtccgcccgc ggtggcctcc gggatcgtgt cagtcactca
    13741 gtgcgtcagc gcgtggagca ggacgttgat ccccacggcg tatccgtcgg gagagaactg
    13801 atagaagaac gcgggatcct cgccttcgcg ctcccacgaa tccgcgatat cggtgttgtg
    13861 cgtcatcacc acgatgatcc ggccgtggtg atcgcgaacg acgcggaagt gcggcacggc
    13921 gctgtcttcg ccgcgttcgg aggtcaccgc gccccgcgtg gaccgccaga actggatgtt
    13981 cgtgatctgc ggcaccttct cgacgacgta ttgggactgg aagatcggat cgctgagcgg
    14041 cacgtcttcg atcgggtatt cgctcggcgg caggaccttg gccagttcgt gcgtccactg
    14101 ttcccaggcc agcggccccc agaaatcgtc cacccagagg aagccgcctt tgagcagata
    14161 cagccgcagg cgctccacct cgtcgtcgtt cagcccgatc gtcccgacgt cggaggccat
    14221 ggtaaacggg cactcgaaca acgcgtcgtc ggtaatccgc acgacgtagt ggttcggctc
    14281 gcgcccgtcg gaccagctga cgggggcccg cgtcagctcc gagaacctga tggtgaggtt
    14341 gatctcgccg tacggatagt ccgtacgcca tcctccgccg gaaggctcgc ttcgcaccga
    14401 cgtataccag agccggcaca cgacgaagtt gcggtcaggg aacagggccg gcgccggccg
    14461 cggggcgcgg tcgaaatagc cgcgtccgaa ctgcgcgaac gccgctccag atgccagcaa
    14521 cgacaccaga aggacgagcg gcagggcgac ggggcgagcg gtccgtgcgc tcatgacggc
    14581 agtgtagcgc ggacggccag tgccggtccg cgcgacaccg tcggaatggc cgctatttca
    14641 tcggagagta gtcggtcggc accaggaact gcgacacgac gcttgcgacg atcttgccgt
    14701 tgacctgtga ttcctgcgcg accttctgcc actcggcatc cttctggaac gcagcccagc
    14761 tctgcttggc ggcatcccga ttggcgtgcg aaatgaggta gacgagcgtg ttctgcgaga
    14821 gcggcgcgtc ctgcggcgtg aagtacgcca cgttcgtcat cccgtgcttc tggaagatcc
    14881 gcatcgtgtg gttccggaat cgcgcgttga gctcgcccag cttgccgtca ggcgccgtgt
    14941 aggttcgaag ctcgaagacg cggccgctct gcgcctgcag cacgccctcg ccgggaacga
    15001 cgccccgcgt caggaacccc agcacgaacg cggccgaaat cgctgcgaca ccgtacgact
    15061 ttctcatggg cagactcctc cttctgtcac gacaggttct ggggaagcgg cacctccgca
    15121 tcccggccaa cgacggtcgt ccattcccgc acccaccatc tggtgacgag cccgttcagg
    15181 acatacgggt cggacctggc gaaatcttcc gcagcctggg gcgactcgcc ccggaacagg
    15241 aacacggcgc cgtcggcggg cttggcgaac gcaccggcga gcaccagctc gccccgtgcc
    15301 tcggcctcac gcgctttggc tatgtggtcg cggcggtacg gggcgcggcg cgccacgtag
    15361 tcctcgacga cgtcatacat gagcagatag tgcatcgccg cggatggtac tacgcgacga
    15421 gcctctcggc taagctagaa ccatgatcaa ggcagtgtct tcatcaaggc ttctttcttg
    15481 tctcctcatt ccgactgttc tggcgacgct cgcctgcggc tcgagccccg agcagagcgc
    15541 cgctccagcg gcgccggcac agcccgcgtc cgacagcggc cgcaagtacc tcctcgagac
    15601 catcgacgac gcggcggtcg tgcagattta cgcggaccag ttctcggcgc tgccgctccg
    15661 ggagaaagtg ctcatctggc acctgtacca ggcggcgctc gccgggcgtg acatcttcta
    15721 cgatcagaaa caccggaacg cgctcgagat gcggcgcgtg ctcgaggcga tcgtcacgca
    15781 cgccgccggc gttgacgggc cgacgctggc ggaaatccag cgctacacga agctcttctg
    15841 gatcaacaac gggccctaca acaacctcac ggcccgcaag ttcgtcctga agacgacgcc
    15901 gcaggccttc gccgcggcgg cgcaggccgc gtccaaggct ggagccacgt tcccggtgca
    15961 ggaaggcgag tctctcgacg cgctcctctc ccggctgcag ccgatgttct tcgaccccga
    16021 ggtcgatcct atcgtcacca acaagacgcc cgggccgggc aaggacatcc tgaccgccag
    16081 cgccaacaat ctgtactcgg gcgtcagcac cgcggacttg cgcggattca cggagcgcta
    16141 tggtctcaac tcgaggctcg tgaagagcaa gaacgggctc gtcgaggagg tctaccgcat
    16201 taacgggcgc tacgggaaag agatcgccag gatcgtcggc cacctcgagg cagccgtgcc
    16261 gcacgcgacc gcgccgatgg ccgcggcgct tcgcgcgctg atccagtggt accgcacggg
    16321 cgaggacagc gaccgggtga agtacgacat cgcgtgggtg caggacaagg actcccccgt
    16381 ggacacgatg aacggcttca tcgaggtcta catggatccg cgggggatca aggggtcctg
    16441 ggaaggcctg gtcttctacg tcaacaggga aaagacggaa cgcatccgta agttcgcgaa
    16501 caacgcgcag tggttcgagg atcggatgcc gtacgacgcc aggtaccgca agccggacgt
    16561 caagggcatc gtcgccaacg ccatcgacgt ggtcatcgag acgggtgatt caggcccggt
    16621 cacgccggtc ggcatcaacc tgcccaacga tcagcggatt cgcgagcagt acggcagcaa
    16681 gtccgtgtcg ctgtcgaacg tgcgggagtc gtcggacaac gcgacgccag ccgccatgcg
    16741 ccaggagttc tcctggaccc cggaggaagt ggagcgcgcg acgaagttcg cggcgctggc
    16801 ggccgagctc aacaccgaca tgcacgaggt gatcggccac gcgtccggcc gtcaggccga
    16861 gggcttcaag ggctcgccgc agagcgtcat caaggagcac ttctcggcac tggaagaggg
    16921 acgcgccgat ctcatcggcc tctatttcgt cgccgacccg aagctcgtcg agctcggcat
    16981 cgtccccgcc gccgaccacg acgcgatcgt ccgcgccgag tacgaggcgt acacacgaaa
    17041 cgccatcgtc cagctccgcc gcatccgcga gggcacgcag atcgaggaag accacatgcg
    17101 gaaccggcag atgatcatcc actggctgat ggccaatacg aaggcgatcg aacggcggca
    17161 gcgcgacggc aagaccttct acgtgatggt ggatcccaag gcgttccacg agggcgtggg
    17221 acggctgctc gtcgaggtgc agcggatcaa gtccgaaggg gacgcagcgg ccgccgcgaa
    17281 gctcttcgat acctacggca ttcacttcga tgcaaagctg cgcgacgagg tcgtcaagcg
    17341 cgtggacgca ctcaagctgc cgtcgtacac cggcttcgtg atgccaaagc tgacgccggt
    17401 gaccgacgcc tcgggaacga tcaccgacgt caggatctcg tacccgatgg acctgacggc
    17461 gcagatgctg gagtacgcgg aaagcacgaa gtgaaacctc ccacttactt tccggctttg
    17521 agctttgaac tttctgtttt cagttcgtct tctcgggaga acatcacgag cggcgttccc
    17581 tcgggcccga tgtagcctcg tcccatcccg gtgacgttga acgacagcgc gacgttgcca
    17641 gcgcggtcga ggccgatgac gccgccttcg cctccggccg ccctgagcac ctcgagcacc
    17701 accgtgtccg cggccgcctg cacgccgagg cccgtgtact ggacgcgcgc acagatgtcg
    17761 tgcgccaccg tgtagcggat gaagtactcc ccctggccgg tcgccgagat ggcgcagctg
    17821 tcgttgctgg cataggtgcc ggcgccgacg atcggtgagt cgcccacgcg gccggagcgc
    17881 ttgttcgtca taccaccggt tgacgtgccg gccgcaaggt tcccgtggcg atcgagcgcc
    17941 acggcgccga cggtgccgaa gtaggcgctc ggcgcgtccc gttccatcgc cggctgcgcg
    18001 gacgcgccct tcttcacgcg ctgcctctcc tcttcgcgcg cgcgctcgag ggcctgccgc
    18061 gcgcgttcgg tgctgaagta gctgttctcg acgaactcga ctccggcgtg atcggcggca
    18121 aaggcttcgg cacccgcacc ggccagcatg acgtgcggtg acttctccat gaccaggcgc
    18181 gccagcagaa tcgggttctt gattcgcgtg acgccggcaa ccgcgcccgc cttgcgagtg
    18241 gcgccgtcca tgatcgacgc gtccagctcg tgggtcccct cgtgggtgaa tacggcgccc
    18301 ttcccggcgt tgaaatgcgg cgagtcctcg aggatgacga tggcagcctg aacggcgtcg
    18361 aggctggaac cgccgccgga caggacttcg tgaccggcct cgagcgcggc ggtcatcgcc
    18421 gcgcgcatcg gttcgcggcg atccccaagg ctctccagcg tgaagtttcc ggccccggtg
    18481 tgaatgacga ggccccatcg aggctgcggt gtcatgtcgg tggaaggcaa ggacggctcg
    18541 ggggccgagc aggacatcac cagcagggcg gcgcaggcgg caagcgcggc ggcgttagag
    18601 cacccgaatg aggatgtcac ggctcgccgt cggacgatcg cccacgttgg cgctcgcctc
    18661 gacgtgaatg acgtagaggc cgggtgcggc gtcttcgagc ggcacctgcg ccaggaatcc
    18721 gtgcccgccc gcgcccgcga gctccttcga cgagcgctcc tctctcgtcg tggcaatgac
    18781 ccgcccggcg tcggtgcgca gctccacctt catttcgatg acatgctcgg cagcactctg
    18841 cagcttttcg tacacctcgg cgtacaccga gagccgatcg ccggcctgga actcgcgagt
    18901 ggcggtgatc ggccctggca actcttcgcg gagcggatcg aacggtcgga ccgtgattcc
    18961 gctccttgcc gagtccgccg tcagcgccac gccgctcatc gtcagccccg ttctcgtgta
    19021 gtcgggcacg tcgaggtcgt acatgacgct gcccgcgcgc gtgggcgtgc cgccggcggc
    19081 gcgaagctgg taacggcccg ggggaagctg catctcggtc aagacacgga cgccgcccgc
    19141 cgtcgtgcgc tcggtggcgt ccttgttgaa gctcactcga gcttcatgcc gcggcccggg
    19201 gtgaatcttg ccgtcggccg acacagccgc caccgcgacg acgacgtcgg tggcgacctt
    19261 accgtccttc tccacgaagc cgagcgcgga cgcgttcatt tcgagcgcga tggccacctt
    19321 cgcccgctgc gtcccgcgct ggtaggacgc cgcgaacaca cgcatgggaa cggctccgtt
    19381 ggggacgggg cttccgagcg actcggcgat tgccggcggg atgcgcccct tctcggccgc
    19441 cgtgagcgga gcggtctgcg ggcggccgcc gagcggcgct aggtatccgc gtcgggcctg
    19501 gacgacgagc cccggccgct tgacgcgcac gtccagctta cggaagcggc cgtcgcggcg
    19561 gtcgttggtt gacgagaagc cgagcacgta gtaccggctg ttgtcgcgca cgatgcgggc
    19621 gaacgactcc tggatgctgt tggtgttgac gacggcgaaa ccgccggttg cctcggcgag
    19681 cgcgcgcaga tcgatcgtcg cctccaaccc gctgaacgct acgaactccc cttcgcccag
    19741 gttgtctccg ccgatcgtca gcccggccgg atcgattggg tagatcgcca cgttcccgcg
    19801 cgtggccgcc gtgatggcgc cgtgcaggtc gccgaaggca atcgacttcg tgccgccggt
    19861 gtagtctagg gcctcgaata catcgaggcc gaggcccgac gtcagcagga tcaacgcctt
    19921 gcgccggccc ttcagcgtgg cggcaaactc gacgagatcg cgcagggcct tcatgcgatt
    19981 gcgaacgagg ttagaccgtt cggcagtgct ggggacggat tcgtacgcga acccacccga
    20041 caccttgtcg gcggcggcga gcagcaggcg acggttgccc gtgaagtcct gcgtgttgcg
    20101 ggactgcccg cggcccatgt agacgatcgc ggcgacgtcg ttcggcgcga actcctgctc
    20161 gatgaaccgg cgaaggaagc ggcgcgtgcg cagcgcctgg acttcgacga tatcgtcgat
    20221 cgcgatgatg tacacgcgcc cttcgccgtc ctcgttcgtg ccgatgtcag gctcgacgtc
    20281 tggcgaggcg agcgaccgtt cgacccgctc gatgggaata tcgacgagcg taaacgagac
    20341 gatcttctgc ggcctgccgt cctcgaacag ctcgaagtcg cccgccgtga ggtcggtcac
    20401 caggttgccg cgctggtccg tcacgaggac gtccacttcg gtggcgtcca cctgggagcg
    20461 gaagacggct ggcggttcct ggggctgctg accctggagg gcgggcgtca caaacggagc
    20521 cacgacggca acaagagcgg ctgtgagtcg gcgtttcaca cgtactccgg gtcgcggggc
    20581 tggcagagcc attctaatcg aacccgatcg tccgtccgtg ccgagccccg gctcaccgct
    20641 tgcgctcgaa cacgacgttc ttggccagca aattcggcgt ggctgtcctg cctgccaacg
    20701 tccacgcatc cgagaacggc cgcccgtccg caggcgtcag cctcggcgtc caccaggcgc
    20761 gatcgatccg cgcaccctgc ggcagcgcga agccaagaag atcttcaatc tgcccaaacg
    20821 tcaggacgac gacgtcggcg taccggtgct cgaggtatct gtggagtgga gcacaatcgc
    20881 cgctcaccac gcgtgggcga ggctctgtcg ccgtcgcctc gggagatgac ttgtcgggag
    20941 cgatcgacgc cagcctcgct gtccgtttcc acatgatgtg ctgatctccc cgacgtcaca
    21001 tgtcgggaga acacatggta ctgcgccttc accgaatggc gtgtgcgatt gtgcacaagg
    21061 catgaacccg cccgccagag ccgcctcccg cctacaggag ggctgggcac gcgggatgtt
    21121 catatttgaa cagcgcctct gacgctcgag tcggcaccat ggcgtctttg caggcgtcga
    21181 cagcgccgcc atgatggaaa agctgaagga actgttcccg acaatcgatc gacgaccggc
    21241 cgctggacga tcgccgacgg cagttggatt cgatgacgcg atgaccgcgc cggccggaaa
    21301 gccacgggcc acggttgatg ccacgatgat ggagaccttc ggatacagcc tccgaatcaa
    21361 gccggatcgc cggcgtggct cttcaaccta tcgcgggggt gaacgccgtc aacgtacgtg
    21421 agcgccgtcc gtccttagcc ccgctcgccg cgaccgcctg gcacgctcgc cgatcgagtg
    21481 cgttcgctct tgaactctaa agcctggaga gcgaggccac cacggagaac cggattggac
    21541 ctcggcggac gaagaggcac gtccgactcg ctcgtcaatc ctggcaaagg cttcgcggta
    21601 aacaggaggc gacacggccg ggcgatgagc cgcgcccacc ggttggctcg attcacgcgc
    21661 gcgctgtccg tcgcgatgcc ggtgttcgcg tacatttgtc gatagagctt cgacagcagc
    21721 acgaacgcga ggcgcgcgcg cagcgttggc gtgcaccggg agcgctccca gacgcggcgg
    21781 agactgtaga accgatccca cacggcctgc gttcgcgcac gaatctcgtc gggcgtcatc
    21841 acggggtgag gtgtgtagac cttcggccgg tgcgcctgcg ggatcagcca gtgccgggtg
    21901 atcggaatac cggccacctt ctgtgcctcg gcgccgacct tcttctccca cgcctcgaag
    21961 tcgactgtgc ccggatacgg cgtcagcatg acgaactgcg cgaaggcgag ctccgcgcgc
    22021 tcggccacat ctgccgtcgc gtcgaacgtc gaaggccggt cgctgggcaa cccgaagatg
    22081 aacgagccga gcacgtacac cccgtgctcg cgaaacttgc ggagccgttg aaccaacgcg
    22141 tcgccgacgt cgttgaagtt cttgtaaatg tccttcaacc cttcgggcgt gacggcttcg
    22201 acgccgacga gcgcgccctt gatattcgcg caccgcatgg cgtcgaggaa cgaatcgtcc
    22261 tcagccgctt ccatcgtgat ctgcgtgaag aacaccatgt ccggcggcag ctccgccagc
    22321 ctcgccatca gctcgaatcg ctcggcccgg agtccccgca gctgctcgag acgcgccacg
    22381 ttgccctgcc gctccgccat ggccaggtcc gccaacgtga ccgggtagaa gttgtcgtcc
    22441 gcgagcgcca cgaagcggaa tccgcgccgg cgcagctcca cgatctccgc cacgacactg
    22501 tcgacgccgc gctgccgggg cttctgtccg tcggtgcgcc acaccgagca gaacgaacag
    22561 tgtttcgggc atccgcgcac ggtctgcacc gagccccaca tatatcggcc ggcgggcagc
    22621 agatcccagc gcgcgggcac gaactggtcg gcgtccacgc gtccaccgtc gtatcgtggc
    22681 tggagcgtgc cctgcacggc atgatcgatc acgagcggcc acaccacgtc gccgtcgccg
    22741 cgaacgacgg tgtgcgcgcc gccacgctcg tacgcctcgt cgggatacag cgtggcgtgt
    22801 atgccgccga agaccaccgt cgccccgcgc gccctcgcgg ccgcaccaag ctcgtagccg
    22861 cgcagcgcat ttcccgtgtg gatcccgatc cccacgatgt cgcccgcgac gatggtgtcg
    22921 agatcgagtg gctcgagcgt ctcatcgacg atgcgtggcc gtccgtattc cggcggcgtg
    22981 gccgcgccca gcacgaacag ccagcgcggc gttatcacgc cgacgccgaa ggacagatgg
    23041 cttgggttta ccaggtggac ggtcatcgca ctccacggcg gacagagagc cccgtcgaat
    23101 tgaaaaccaa atgtgagaaa gcgactgcgg ccgacatcgt cacgctcctg cacggcagct
    23161 cattggctcc cggaagctcg gaggctgaag cgacgtcacg atggcgtggc acccgtctgg
    23221 cgggcaattg tagtctacag tacatttggg cgcggttggc ctgattcccg ccgcgacgcc
    23281 cgcgtgggcg tactctgaac gaggcgactc gtgtgaatgc aaccgcggcc gttcgccgcc
    23341 acgtcgttcg gcctgaaccc aagagtcagg aggtcatcat ggatccagca gaagcacagg
    23401 cgagggtgat cattgccgcg gcattgattg tcagaggcgc ggtggaggtc ccggccatgc
    23461 cctccggcag tcagtatgtg ccggatccgg caggagtgcg cctgcgtcag cttaccgatt
    23521 acgtgtatcg gcttctcaca atcgacgagc cgcaagaggc gcggtaaatg ggcagcgttc
    23581 gctgaggtcc aacgggacag ccacgatcgg gatatacttg ccgctcctgc ttttctgagc
    23641 cctagggcca ttccggtcca gccctcacca aaagcctttc gcgtgggggt cacatggcca
    23701 tcgacgtttc caccgttgcc gcgcccccac aggcggctat ttccacgcca gcacctgagt
    23761 cagtaggttt cgatgaaagg tgggctgctt ggcaggcgaa gggggccgcg cacgatcggg
    23821 ctgtccgtcg aaagatcgcg gtcgccgcgc ccatactgat cgtcagcgcc gccgtcgtct
    23881 tctacgtgct gctcggacgc tgagcggagg ccatcgatgg tggcgaccgc gggtgagctc
    23941 acgcgtgacc tcgagggact ggtcggggcc ggacagtggc gagccgaccc aggcagtgcg
    24001 tagagaagag aggatgccag acgcgcgagt gcgttcgatc cgtctcctgc ctgacggaac
    24061 ccctttgcac aatcggctcc tcgccgctct gccacgtagc gaatacgacc ggatattgcc
    24121 ctacctccga atgagcaccg tgaccattgg cgagaccctg caggcgcacc gcgtgcgaat
    24181 caaggacgtc tacttcccga atggcggtgt cttctccgtc accaacgaaa tgcggaacgg
    24241 ggggctcgt
//
LOCUS       DBNGSH010000086         1852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1024238, whole genome shotgun sequence.
ACCESSION   DBNGSH010000086 DBNGSH010000000
VERSION     DBNGSH010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1024238"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="AC16CY_02000"
     CDS             <1..737
                     /locus_tag="AC16CY_02000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3352576.1"
                     /translation="RSWAAMLSTEGRSREIYEAWHPHVESDARDDAPWHDLIARHATP
                     YDLADRRVLEIGCGRGELVRRLMSAVAGPRHLVAADFAQSAVRLGRLRTGECGHRVSW
                     AVASIQDIPLPDSTFDTVISCETIEHVVDPLKALREVRRVLRPGGRLLLTTPNYLGPM
                     GLYRGYLRARGRRYTEGGQPICHFTSLPRTMSWLRRAGLHVLEVDAVGHYLPWPGRTP
                     LTLPGLDSVRLLRWFALHSLVVAERP"
     gene            737..>1852
                     /locus_tag="AC16CY_02005"
     CDS             737..>1852
                     /locus_tag="AC16CY_02005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3352577.1"
                     /translation="MRVLFFVEGFTDIRFVVGLSEICDLTMAVPERAYVESTLEERVA
                     RSGAHLAVHEIPGGRLAFQARSFAYLWKVAGDFDVILSQEVLRGSLNATIVGAVRGVP
                     VVTTMALPPVEYFRCRRERGHIGPGAAWAGELAIRTMMSINGRLATRCLALGPYLEGI
                     ASEVCPRTETGYYYGVDVDRFRPASEEERRRLRARLSLPAHKFLIVLASRISHEKDPE
                     TVLRAVALARARGLDAVLLNLGGGYQEFVALAGSLGIADADAWVLGRPAVHPMQELAD
                     YFRAADLLVQGSLEEGLGLSPLEALACETPVVATAVGGMAAHLGPYAALTPRRDAEAM
                     AEAILEVDADPTLARIRARHGREYVREYWSRERAFREL"
BASE COUNT          261 a          622 c          648 g          321 t
ORIGIN      
        1 ggcggtcatg ggcagcgatg ctgtctactg aaggacgctc gcgcgagatc tacgaggcgt
       61 ggcatccgca cgtcgagtcc gacgcgcgtg atgacgcgcc gtggcacgac ctcatcgcaa
      121 gacatgcaac cccgtatgac ctcgctgacc gccgtgtcct ggagatcggg tgcgggcgtg
      181 gcgagctggt ccgtcgcctg atgtccgccg tcgcgggccc acggcatctg gtcgccgccg
      241 actttgccca gtcggccgtt cggcttggcc ggctcagaac gggggaatgc ggccaccgcg
      301 tgtcgtgggc ggtggccagc attcaggata ttccgctgcc ggactcgacc ttcgacaccg
      361 tcatctcatg cgagacgatc gaacacgtcg tcgatccgct caaggcgctg cgagaggtgc
      421 ggcgcgtact tcgtccgggc gggcgtctgc tgctgacgac gcccaactat ctgggtccga
      481 tggggctcta ccggggatac ctgcgggccc gcggccgtcg atacaccgag ggcggccagc
      541 cgatctgtca cttcacgtcg ctgcctcgaa cgatgtcgtg gctgcgacgc gccggcctcc
      601 acgtgctcga ggtcgatgcc gtcggccact atcttccgtg gccaggacgc actccgttga
      661 cgctgcccgg tctcgactct gtccgtctgc ttcgctggtt cgcgctgcat tcgctggtgg
      721 tcgctgagag gccatgatgc gggtcctctt cttcgtcgaa ggcttcaccg acatccgctt
      781 cgtcgtcggc ctcagcgaga tctgcgatct cacgatggcc gttccggaaa gagcctacgt
      841 cgagagcacg ctcgaggaac gcgtggcgcg cagcggcgcg catctcgcgg tgcacgagat
      901 tccgggcggg cgcctggcct tccaggcgcg atcgttcgcg tacctctgga aggtcgcggg
      961 ggatttcgac gtcatcctgt cgcaggaggt gcttcgcggc tcgctcaacg ccaccatcgt
     1021 cggcgcggtc cgcggcgtgc ctgtcgtgac gaccatggcg cttccgccgg ttgagtattt
     1081 caggtgccgg cgcgagcgcg gccacatcgg cccgggcgcg gcctgggcgg gggagttggc
     1141 catccgcacg atgatgagca tcaacgggcg gctcgccacg cgctgcctgg cgctcggacc
     1201 gtatctcgaa gggattgcct cagaagtgtg cccccggacc gagacggggt actactacgg
     1261 cgtcgacgtc gatcggtttc gacccgcgag cgaggaggag cggcgccggc tccgcgcgcg
     1321 gttgagcctg cccgcgcaca agttcctgat cgtgctggcc agccgcatca gccacgaaaa
     1381 ggatccggaa accgtactcc gggccgtggc cctcgcgcgc gcccgcgggc tcgacgccgt
     1441 cctgctcaat ctcggcgggg gctaccagga gttcgtggcg ctcgccggct cgctcggcat
     1501 cgctgacgca gacgcgtggg tgctcggccg gccggccgtg cacccgatgc aggaactggc
     1561 ggactacttc cgcgccgccg accttctcgt gcagggatcg ctcgaggaag gtctcggact
     1621 gtctccgctg gaagcgctcg cgtgcgagac gccggtcgtc gccacggcgg tgggaggaat
     1681 ggccgcgcac ctcggtccgt acgcagcctt gacaccgcgt cgtgacgccg aggcgatggc
     1741 ggaagcgatt ctcgaggtcg atgccgaccc gaccctggcc aggatcaggg cccggcacgg
     1801 ccgcgagtac gtccgcgagt actggagccg cgagagagcg tttcgcgagc tg
//
LOCUS       DBNGSH010000087         1578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1364152, whole genome shotgun sequence.
ACCESSION   DBNGSH010000087 DBNGSH010000000
VERSION     DBNGSH010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1364152"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /gene="dacB"
                     /locus_tag="AC16CY_02010"
     CDS             <1..462
                     /gene="dacB"
                     /locus_tag="AC16CY_02010"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR3352578.1"
                     /translation="LMRLNADRGMFPTNDAALEGLRRRLRSWGIAEDSCQLVDGSGLS
                     RRDAISPEALVIVLQRMFDASAASPFMAALPIAGIDGSLETRMKGTAAQGSVRAKTGT
                     MSNIRSLAGYATTRGGERLAFVIVVNNFEGTGAEANQAVDAIAVRLAEFTR"
     gene            complement(482..>1578)
                     /locus_tag="AC16CY_02015"
     CDS             complement(482..>1578)
                     /locus_tag="AC16CY_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR3352579.1"
                     /translation="GQPFDQGRLNCLAPSHLRTLTLDVVETIDEGDDILHVVSAPIPA
                     GTRVHGEIDWSRRFDHMQQHSGQHVLSAAFEKLFTNRTMSFHMGTDTSTIDLAREASV
                     SEIDRAVDEANRVVWDDRAVSVRFVSAEEASRLQLRKEPGREGTLRLVEIADVDLSAC
                     GGTHVPRTGAIGMIGVLGAERFRGGSRVTFVCGGRASRVLRSYRDAIAGSVRVLSVLP
                     DELPAALERIQLEAKQLRKTIGRLQDTLAGHEAERMLSGAPSIEGVRVVVQSIEGWDA
                     AGLKAMASALTNQSRVAAALFTRGDPIAAVIARSPDVELDASKVLRALLDRFGGRGGG
                     KPDLAQGGGLGGHVDEILSSARELLTRRSG"
BASE COUNT          266 a          528 c          526 g          258 t
ORIGIN      
        1 ctgatgcgtc tcaacgcgga caggggaatg tttcccacca acgatgcggc gctcgagggc
       61 cttcgccggc ggctgcgatc gtggggcatc gccgaggaca gctgccagct cgttgacggc
      121 tccggcctct cccggcggga cgcgatctct cctgaggcgc tcgtgatcgt actgcagcgg
      181 atgttcgacg cgtccgccgc atcgccgttc atggccgcgc tgccgattgc cggaatcgac
      241 ggctcgctcg agacgcggat gaagggcacg gcggcgcaag gcagcgtgcg cgcgaagact
      301 ggcacgatgt ccaacatccg ctcgctggcg ggctacgcga ccacgcgcgg cggcgagcgg
      361 ctggcgttcg tgatcgtggt gaacaacttc gaagggacgg gtgccgaagc gaatcaggcg
      421 gttgatgcaa ttgccgtccg tctggccgaa ttcacacgct aggcgtacgt agtgtccggc
      481 tttagccgga ccttcgggtg agtagttccc gcgcggacga gagaatctcg tcgacgtgtc
      541 cgcccagccc tcctccttgc gcgagatcgg gcttgcctcc gccccgtccc ccgaagcgat
      601 cgagcagcgc gcgaagaacc ttgctcgcat cgagctcgac atccggcgaa cgcgcgatca
      661 ccgcggcgat cggatcacct cgagtaaaga gggccgcggc gacgcggctc tggttcgtca
      721 acgccgacgc catcgccttc aaccccgcag cgtcccatcc ctcgatcgac tggacgacga
      781 cgcggacacc ttcgatcgaa ggggccccgg acagcatgcg ctccgcctcg tggccagcca
      841 gcgtgtcctg gagcctgccg atggtcttgc ggagctgctt cgcctcgagc tggatccttt
      901 cgagtgcggc aggcaactcg tctgggagta cggacaacac ccggacgctg cccgcgatcg
      961 cgtcgcgata cgatcgcaga acccgggacg cgcgcccgcc gcacacgaac gtcacccgcg
     1021 aaccgccgcg aaaccgctcg gcgcccagga cgccgatcat tccgattgcc cccgttcgcg
     1081 gcacgtgggt tccgccgcag gccgagagat cgacgtcggc gatttcgacg agcctgagcg
     1141 tgccctcgcg acccggctcc ttgcgcagtt gaagacgtga cgcctcctca gcggacacga
     1201 agcggaccga gacggcgcga tcgtcccaga caacgcggtt ggcctcgtcc accgcgcgat
     1261 cgatctctga cacggatgcc tcgcgtgcca ggtcgatcgt ggaggtgtcg gtgcccatat
     1321 gaaagctcat cgtccggttg gtgaacagct tctcgaaagc ggccgagagc acatgctggc
     1381 ccgagtgctg ctgcatgtgg tcgaagcggc gcgaccaatc gatctcgcca tgcacgcggg
     1441 ttcctgccgg gataggagcg ctgaccacgt ggaggatgtc gtcgccttcg tcgatcgtct
     1501 cgacgacatc cagagtgagg gtgcgaaggt gcgaaggtgc gaggcaattg agccggccct
     1561 ggtcgaaggg ctggccgc
//
LOCUS       DBNGSH010000088         1873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2213615, whole genome shotgun sequence.
ACCESSION   DBNGSH010000088 DBNGSH010000000
VERSION     DBNGSH010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2213615"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="AC16CY_02020"
     CDS             <1..1549
                     /locus_tag="AC16CY_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3352580.1"
                     /translation="ALMTPEMDASSIALGRKTTVISNIMQAEWMWGRRGRTAGDKDPK
                     ELLYEQCYGLFQGSPVPEMKERRREKLTLDALEDLIEHLGGLREGRKAVLTVTEGWRL
                     FGPNYKLSGMNSGINSGGVSVECEADRLTLASLDHTQRLRDLTDLANRDNLTFYPVYA
                     RGLVVFDAPVGPDAPPSLRQDGANLRERQDGLKFLADYTDGTWIVNTNNIDGALQRIV
                     ADLSSYYLLGYNSTNSKLDGRFRNITVRVKRPGVRVRARRGYRGRTADDVITPGAAER
                     PSPVSSALTPVVGVSARSLFRIRASSWVRPGSGGAASGAFWVVGELDYRTRRELAWTA
                     SAKAEVVVVAADGKEIMARTLDVKTSEGPFSFDVPESGDLPAGEYAVRVRLKSDADNT
                     LSLSDTARVVLDKPAALSEAVMWRRGPTTGLQHRRTADPRFQRSERIRLELATTTAAP
                     AVARLLDRSGNPLAIPVQVSARPDPTGAFQWIVVDASLAPLAPADYAIEVTQGTDKQL
                     TGFRVVP"
     gene            complement(1555..>1873)
                     /locus_tag="AC16CY_02025"
     CDS             complement(1555..>1873)
                     /locus_tag="AC16CY_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR3352581.1"
                     /translation="VFNSPSELETDVSPSARIRVQFSRGLNEASLEGNIRVSYAGQTA
                     EAGAPGLPFKVVYDGANRAIQIAMAQPFEPNRTVRVELLEGIKGFDDAPVTPWTLTFS
                     VAF"
BASE COUNT          317 a          610 c          633 g          313 t
ORIGIN      
        1 ggcgttgatg acgccggaga tggacgccag ctcaatcgcg ctcggccgta agacgactgt
       61 catttccaac atcatgcagg ccgagtggat gtggggccgg cggggccgca cggctggaga
      121 caaggacccg aaggagctgc tctacgaaca gtgctacggc ctcttccagg ggagcccggt
      181 gcccgaaatg aaggaacgcc ggcgcgaaaa gctgacgctc gatgcgctcg aggatctcat
      241 cgaacacctt ggagggctgc gcgaggggcg caaggccgtg ctgacagtca ccgaaggttg
      301 gcgcctcttc ggtccaaact acaagctgtc tggcatgaac tcgggcatca actcgggagg
      361 agtcagcgtc gagtgcgagg cggatcggtt gaccctcgca agtctcgacc acacgcagcg
      421 cctgcgggat ctcaccgacc tggccaatcg cgacaacctc accttctacc cggtgtatgc
      481 gcgtggcctg gttgtgttcg acgctcctgt cggtcccgat gcgccgccct cccttcgtca
      541 ggacggggct aaccttcgcg aacggcagga tggcctcaaa ttcctcgccg attacacgga
      601 tggcacctgg atcgtcaaca cgaacaacat cgacggggcg ctccagcgaa tcgtcgcgga
      661 tctgtcgtcc tactatctgc tcggctacaa ctcgacgaac tcgaagctcg atggccgttt
      721 caggaacatc acggtccgcg tgaagcgtcc gggcgtgcgg gttcgcgcgc gccgtggcta
      781 tcgcggccgc acggccgacg acgtgattac ccccggagcg gccgagcggc cgagcccggt
      841 ttcgtcggcc ttgacacctg tcgtcggagt cagcgcacgc tcgctattcc gcattcgtgc
      901 ctcgtcctgg gtaaggccgg gatccggggg ggcggcgtcc ggcgcgttct gggtcgtcgg
      961 cgagctggac taccggaccc ggcgcgagct ggcctggacg gcgagcgcca aagcggaggt
     1021 cgtcgtcgtg gccgccgacg gcaaggagat catggcccgc acgctggatg tgaagacctc
     1081 agagggcccg ttctcgttcg acgttccgga atcgggcgac cttccagcgg gtgagtacgc
     1141 cgtccgcgtc cgtctcaagt cggacgcaga caacacgctc agcctgagcg ataccgcgag
     1201 ggtcgtactc gacaagcccg cggctctcag cgaggccgtg atgtggcgtc gcggaccgac
     1261 gacggggctg cagcaccgcc ggaccgccga cccgcggttt cagaggagcg aaagaatccg
     1321 gctggagttg gcgacgacga cggctgcgcc cgctgtcgcg cggctgctcg accgctcggg
     1381 caaccccctg gcgatccccg tgcaggtgtc cgcgcgcccg gacccgaccg gcgcgtttca
     1441 atggatcgtg gtggacgcgt cgctggcgcc gctcgcgccc gccgactacg ccatcgaggt
     1501 cactcaaggc actgacaagc agttgaccgg gttccgcgtc gtcccctgag tcggtcagaa
     1561 cgcgacgctg aaagtcagcg tccacggggt gaccggagcg tcgtcgaacc ccttgatgcc
     1621 ctcgagcagc tctacgcgga ccgtgcggtt cggctcgaat ggctgcgcca tggcgatctg
     1681 gatcgcgcga ttggctccgt cgtagacgac cttgaacggc aggcccggcg cgccggcttc
     1741 agccgtctgg cccgcgtagc tgacgcggat gttcccttca agggaagctt cgttcagtcc
     1801 ccgcgagaac tgaacgcgga ttcgcgctga agggctgacg tcggtttcga gctcgctggg
     1861 cgagttgaag acc
//
LOCUS       DBNGSH010000089         6335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2723007, whole genome shotgun sequence.
ACCESSION   DBNGSH010000089 DBNGSH010000000
VERSION     DBNGSH010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2723007"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="AC16CY_02030"
     CDS             <1..605
                     /locus_tag="AC16CY_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3352582.1"
                     /translation="EVKDRVVAMMRDIRMGDVADFRTFMGAVIDRKAFAKISGYLDDA
                     RRNATIVQGGGARDEVGYFIEPTLVEASDPGYRLLCEEIFGPVVTAYAYPDAQWEQTL
                     DVVDRTSPYALTGAVFARDRGAVLQASSALRNAAGNFYINDKPTGAVVGQQPFGGARA
                     SGTNDKAGSKMNLLRWVSARAVKETFSPPREYLYPYMTEQ"
     gene            complement(683..1492)
                     /locus_tag="AC16CY_02035"
     CDS             complement(683..1492)
                     /locus_tag="AC16CY_02035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3352583.1"
                     /translation="MRSLLLTLTVLAAAPAAAQTLAPPPDELPAKEVAVPGDGEKSQT
                     GLVSKQVTRGRSAEKPSATDVVTVHYTGWSSEGRLFDSSIVRGTPSTFPLDRVMAGWR
                     ECVLLMTLGEKRRCWIPESLAYKGQSGRPQGTVVFDIELLDTRPSPLTAPADVKGPPK
                     DAKRTSTGLAYVVLRPGTGARNPSRFGRVTVHYTGWTTEGRMFDSSVTKGTPATFALD
                     GVIKGWTEALPLMVEGERTRFWIPEKLAYDGKGDGPRGNLVFDIELIRIEN"
     gene            complement(1515..2618)
                     /gene="sucB"
                     /locus_tag="AC16CY_02040"
     CDS             complement(1515..2618)
                     /gene="sucB"
                     /locus_tag="AC16CY_02040"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR3352584.1"
                     /translation="MSNIVVPELGESVVEARVARWLKKAGDRVEAGEPVVELETEKID
                     LEVGAERSGVLASIARKEGEDVRIGELLAVIDESAAPAPASAAPATAGKPTGGNGSGG
                     TAPASAVPATAGEPTKVEIAPAFAVSATAGRPERTEERVRMSKRRLTIARRLVEAQHT
                     AAMLTTFNEVDMTAVMGLRERQKDEFKKQFGVGLGIASFFVKSAVAALRAFPALNAEI
                     QGDEIVYKRYYDIGVAVGAEGGLVVPVIRDADRLSFAQIEQSIRDFAARAAGGSLTLE
                     DLKGGTFTVTNGGVFGSLLSTPILNPPQVGILGLHKIQDRAVPVEGRVAIRPMMYVAL
                     SYDHRIVDGREAVQFLVKIKSCVEDPGRLLLEA"
     gene            complement(2630..5491)
                     /locus_tag="AC16CY_02045"
     CDS             complement(2630..5491)
                     /locus_tag="AC16CY_02045"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR3352585.1"
                     /translation="MTAGPFGGVNAAYVQELYERYRQNPESVDPASRAAFERWTPPDE
                     FALTHGPAKAGHSMGDSAPAGGPTADVHAVVSATILADSIRRYGHLAARLDPLGSEPI
                     GDPSLSPATYGLSDDDLKRLPASVVGGPVAETSATAFDAIEKLRRVYCSTTGFDYSHI
                     FIPEERDWLRHAAESGRFLPPLDPESTASLLDRLTRVEVFERFLHRTFPGKTRFSLEG
                     LDMLVPILDEIISGAAETGARHTIIGMAHRGRLNVLAHVLEKPYAQILAEFKDPVLAA
                     RLRIDLGWMGDVKYHAGARTTRPRGHTFVTMPPNPSHLEAVNPVVAGMARAAGTYANE
                     PGPGTFSHAMTLPVLIHGDAAFPGQGIVAETLNLSRLEGYDTGGTIHIIANNQLGFTA
                     TPEQSYSTSYASGLARGFKIPIVHVNADDPAACLEAAWLAWEYRARFARDFLIDLVGY
                     RRHGHNEGDEPAFTQPLMYKTIASHPTVREQFARTLVSQGTQTAESVEALVRRHFAVL
                     EKALASLKPEEDFVPSVPEPAPPGAAAKVETAVPIERLREINEGLQRAAPSGFVFHKK
                     LERSRERRRLSLDNPAERTIDWSTAEELALASILADGTPVRVTGEDVERGTFSQRHSV
                     FHDAETGRRFIPLQAFAQATAAFEIHNSPLSEAGAVGFELGYNMQEPDRLVVWEAQYG
                     DFINGAQVIIDQFVTSGRAKWGLQPSLVLLLPHGHEGQGPEHSSARPERFLQAAADIN
                     MRVVNCTTPAQYFHLLRRQAALLEKDPLPLVVLTPKSLLRHPMVMSTPHELATGRFRS
                     VLDDAEARTRASGVRRVVLCSGKVYVDLVSGERRQSASGVAVCRVEQLAPFPRVALRE
                     VLEGYSSLEEIVWLQEEPENMGAWEWVRGQIDETIADRWLLRYIGRVRSASPSEGSAA
                     WHQINQRILVDEAFSLDGPAAGSSTILSKPVRSLTAAKR"
     gene            5552..>6335
                     /locus_tag="AC16CY_02050"
     CDS             5552..>6335
                     /locus_tag="AC16CY_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR3352586.1"
                     /translation="MIRLIGIDIDGTLLDSGGHLPAANVTAIAAAVAAGVHVALVTGR
                     SYPFARPVANDLPGAITLIVSNGAVERGMDGSTLARRLLDRGVARDVLDATRGHRHAA
                     ALVFDRDADRQVVYETMDWEHPGRRRYWSRNHAHIAQSVPLEDALTEDPIQVMFNGGV
                     DPMRELWSSLRADLRGCSILMTEYVSRDFSLVDVTSPDATKGRALAWRARELGLTRDE
                     VMAVGDNFNDVEMLEFAGHPVVMANAVAELKSRGWHVTGHQDE"
BASE COUNT          967 a         2153 c         2167 g         1048 t
ORIGIN      
        1 gggaggtcaa ggaccgcgtt gtcgcgatga tgcgcgacat ccgcatgggc gacgtcgcgg
       61 acttccgcac gttcatgggc gcggtgatcg accgcaaggc gttcgcgaag atcagcggct
      121 acctggacga cgcgcggagg aacgccacga tcgtccaggg agggggcgcg cgggacgagg
      181 tggggtattt catcgagccg acgctcgtcg aggcgtccga cccgggctat cggctgctgt
      241 gcgaggagat cttcggcccg gtggtcacgg cctatgcgta ccccgacgcg cagtgggaac
      301 agacgctcga cgtcgtcgat cgaacctcgc cgtatgcgct gacgggcgcg gtgttcgcgc
      361 gcgatcgcgg cgccgtcctc caggcgtcga gcgcgctgcg caacgcggcc ggcaacttct
      421 acatcaacga caagcccacg ggagccgtcg tggggcagca gccgtttggc ggcgcccgcg
      481 cgtcgggcac caacgacaag gcgggctcga agatgaatct tctccgctgg gtgagcgcgc
      541 gggccgtgaa ggagacgttc tcgccgccgc gggagtactt gtacccctac atgacggagc
      601 aatgaagagg tccggctaaa gccggacact acttctttct ggtacgtagt gtccgccttc
      661 aggcggacct cagcgcgtcc gatcagttct cgattcggat cagctcgatg tcgaacacca
      721 ggttcccgcg cggaccgtcc cccttgccgt cgtaggcgag cttctccgga atccagaagc
      781 gcgtgcgctc gccttcgacc atcaacggca gcgcttcggt ccatcccttg atcacgccgt
      841 cgagcgcgaa ggtggccggg gtccccttcg tcaccgagct gtcgaacatc cgcccttcgg
      901 tggtccaccc ggtgtagtgc acggtgacgc gtccgaaacg gctcgggttg cgcgcgcccg
      961 tgcccggccg caggacgacg tacgccagcc cggtcgaggt ccgcttcgca tctttcggag
     1021 ggcctttcac atccgcggga gccgtcagcg gcgacggtct cgtgtccagc agctcgatgt
     1081 cgaagacgac cgttccttgc ggacgtccgg actgtccctt gtaggccagg ctttcgggga
     1141 tccagcagcg gcgcttctcg ccgagcgtca tcagcagcac gcactcgcgc cagccggcca
     1201 tcactctgtc gagagggaac gtggacggcg tcccccgcac gatcgagctg tcgaacaggc
     1261 ggccctcgct ggaccagccg gtgtagtgga ccgtgacgac gtcggtggcc gacggcttct
     1321 cggcgctcct gccccgagtc acctgcttcg agacgagacc cgtctggctc ttctccccgt
     1381 cgccggggac ggccacttcc ttcgccggca gctcgtcggg cggcggggcg agtgtctggg
     1441 cagcggccgg agctgcggcg agaacggtga gcgtcagaag caggctacgc atatctgtaa
     1501 gcattcaaca tcgatcatgc ctcgaggagc aaccgccccg gatcttcgac gcagctcttg
     1561 atcttcacca ggaactgcac ggcctcgcgg ccgtccacga ttcgatggtc gtaggaaagg
     1621 gcgacgtaca tcatcggccg gatggccacc cggccttcga caggcacggc gcggtcctga
     1681 atcttgtgca ggcccagaat gcctacctgt ggagggttga ggatcggcgt gctcagcagc
     1741 gacccgaaca cgccgccgtt ggtgacggtg aaggtgccgc ccttcaggtc ctccagcgtg
     1801 agcgatccgc cggcagcgcg cgcggcgaaa tcccggatgg actgctcgat ctgcgcgaac
     1861 gacagccggt cggcgtcgcg aatcaccggc acgacgagcc cgccctcggc gcccaccgcc
     1921 acgccgatgt cgtagtagcg cttgtagacg atctcgtccc cctggatctc ggcgttgagg
     1981 gcggggaacg cgcgcagcgc ggccaccgcg gacttcacga agaaggaggc gatgccgagg
     2041 cccacgccga actgcttctt gaactcgtcc ttctggcgtt cgcggaggcc catgaccgcc
     2101 gtcatgtcca cctcgttgaa cgtcgtcagc atggcggccg tgtgctgcgc ttcgaccagg
     2161 cggcgcgcga tggtcaggcg gcgcttggac atgcggacgc gttcttcggt gcgctccggc
     2221 ctgcccgccg tagccgaaac ggcgaaggcg ggtgcgatct cgaccttcgt aggctcgccc
     2281 gccgtcgccg gaacggcgga ggcgggtgcc gttcctcccg atccgttgcc gcctgtcggc
     2341 ttgcccgccg tagccggagc ggcggaggcg ggcgcaggcg cggcgctctc gtcgatcacc
     2401 gccagcagct ctccgatcct gacgtcctcg ccctccttgc gggcgatgct ggcgaggacc
     2461 ccgcttcgct cggcgcccac ttccaagtcg atcttctcgg tttcgagctc cacgacgggc
     2521 tcgcccgcct cgacgcgatc gcctgccttt ttcagccacc gggccacgcg cgcctcgacg
     2581 accgactctc ccagctccgg tacgacgatg ttggacataa caggaggtct cagcgtttcg
     2641 cggcggtcag gcttcgaacc ggcttcgaga gaatcgttga cgagccggcg gcggggccgt
     2701 cgagcgagaa cgcctcgtca acgaggatcc gctggttgat ctgatgccac gccgccgagc
     2761 cctccgacgg gctcgcgctg cggacgcggc cgatgtagcg aagcaaccag cggtcggcaa
     2821 tcgtctcgtc gatctggccg cgcacccatt cccacgcgcc catgttctcg ggctcttcct
     2881 gcagccacac gatctcttcg agcgatgagt atccctcgag gacctcgcgc agcgcgacgc
     2941 gcggaaacgg cgcgagctgc tccacgcggc agacggcgac cccacttgca gactgccgcc
     3001 gctcgcccga gacgaggtcc acgtacacct tgccgctgca cagcaccacg cgcctcacgc
     3061 ccgatgcccg cgtccttgcc tccgcatcgt cgagcacgga gcggaaccgg ccggtggcca
     3121 gctcgtgcgg cgtcgacatc accatcggat gccgcaggag gctcttgggc gtcagcacga
     3181 cgagcggcag aggatccttc tcgagaagcg ccgcctgacg gcgcagcaga tgaaagtact
     3241 gagcgggagt cgtgcagttg accacccgca tgttgatgtc ggcggccgcc tggaggaagc
     3301 gctccgggcg cgcgctcgag tgctcgggcc cctgaccttc gtggccgtgc ggcagcagga
     3361 gcacgagcga gggctgcagc ccccacttgg cgcgccctga cgtgacgaac tggtcgatga
     3421 tcacctgggc gccgttgatg aaatcgccgt actgcgcctc ccagacgacc agccgatccg
     3481 gctcctgcat gttgtagccg agttcgaatc cgacggcgcc cgcttcgctg agcgggctgt
     3541 tgtggatctc gaaggccgcc gtcgcctgcg cgaacgcctg gagcggaatg aagcggcgcc
     3601 ctgtctcggc gtcgtggaag accgagtgac gctggctgaa cgtgcctcgc tcgacatcct
     3661 cgccggtcac cctcactggc gtgccgtcgg ccaggatgct cgccagcgca agctcctccg
     3721 ccgtggacca gtcgatggtc cgctccgccg ggttgtcgag cgacaggcgc cttcgttccc
     3781 ggctgcgctc gagcttcttg tggaacacga agcccgatgg cgcggcccgc tgcagccctt
     3841 cgttgatttc gcgcagccgc tcgatgggca cggccgtctc gaccttcgcg gctgcgccag
     3901 gcggcgccgg ctccggcacc gagggaacga aatcctcctc cggtttgagc gatgcgagcg
     3961 ctttctcgag caccgcgaag tgcctcctga cgagggcctc gaccgattcg gccgtctgcg
     4021 tgccctgcga cacgagcgtc cgggcgaact gctcgcgcac cgtgggatgg gaggcaatcg
     4081 tcttgtacat caacggctgc gtgaacgccg gctcgtcgcc ttcgttgtgg ccgtgccgcc
     4141 tgtagccgac gaggtcgatc aggaagtcgc gcgcgaaccg cgcgcggtac tcccacgcga
     4201 gccacgccgc ctcgagacag gccgccggat cgtccgcatt gacgtgcacg atcgggatct
     4261 tgaacccgcg cgccagcccg ctggcgtagc tcgtgctgta ggactgctcg ggcgtcgccg
     4321 tgaacccgag ctgattgttg gcgatgatgt gaatcgtgcc gccggtgtcg tagccttcga
     4381 gccgcgagag gttgagcgtt tcggccacga tgccctgacc ggggaacgcg gcgtcgccgt
     4441 ggatcagcac cggaagcgtc atcgcgtggc tgaacgtccc cggccccggt tcgttcgcgt
     4501 aagtcccggc ggcccgagcc atgccggcga ccaccgggtt gaccgcctcc aggtggctcg
     4561 ggttcggcgg catcgtcacg aaggtgtgac cccgcggccg cgtcgtccgc gcgccggcgt
     4621 ggtacttcac gtcgcccatc catccgaggt cgatgcgcag ccgcgcggcc agcaccgggt
     4681 ccttgaactc ggcgaggatt tgcgcgtacg gcttctcgag gacgtgcgcg agcacgttga
     4741 ggcgaccgcg gtgggccatg ccgatgatcg tgtgacgtgc gcccgtttcc gccgctcccg
     4801 agatgatctc gtcgaggatc ggcacgagca tgtcgaggcc ttccagcgag aaccgcgtct
     4861 tgcccgggaa ggtccggtgc aggaagcgct cgaacacctc gacacgcgtg agccggtcca
     4921 ggagcgaggc cgtgctctcc ggatcgagcg gcggcaggaa ccgtcccgat tcggcggcgt
     4981 gccgcagcca gtcgcgttcc tcggggatga agatgtgcga gtagtcgaag cccgtcgtcg
     5041 agcagtagac gcggcgcagt ttctcgatcg cgtcaaacgc cgtggccgat gtttccgcca
     5101 ccggtcctcc cacgaccgac gccggcagcc gcttgaggtc gtcgtcggag aggccgtagg
     5161 tggccggcga cagtgaggga tcgccgattg gctccgagcc gagcgggtcg agccgggcgg
     5221 ccaggtggcc gtatcgccga atcgagtcgg cgaggatggt cgccgagacg accgcgtgga
     5281 cgtcggctgt cggtccaccg gccggcgcgc tgtcgcccat ggagtgtccg gctttagccg
     5341 gaccgtgcgt gagggcgaat tcgtcagggg gcgtccacct ctcgaacgcg gcccgcgagg
     5401 ccggatcaac cgactcggga ttctggcggt agcgctcgta caactcttgt acatacgcgg
     5461 cgttgacgcc cccgaacggc cccgctgtca tgacctgtct atcgtatcgc acgggctggg
     5521 acagaatagg gattgggcgc ggaattgctc aatgattcgt ctgatcggca tcgacatcga
     5581 cggcaccctg ctcgatagcg gtgggcacct gccggcggct aacgtcaccg cgattgccgc
     5641 cgccgtcgcc gccggcgtgc acgtggcgct cgtgaccggg cgcagctacc cgtttgcgag
     5701 gccggtggcc aacgatctcc ccggcgcgat cacgctcatc gtcagcaacg gcgccgtcga
     5761 acgggggatg gacggctcga cgctggccag gcggctgctc gatcgcggcg tcgcacgcga
     5821 cgtgctcgac gcgacgcgcg gccatcgtca cgcggcggcg ctcgtcttcg atcgcgatgc
     5881 cgaccggcag gtcgtctacg agacgatgga ttgggagcat ccgggacggc ggcggtactg
     5941 gtcgcgcaac cacgcgcaca tcgcgcagtc ggtgccgctc gaggacgcgc tcaccgagga
     6001 cccgattcag gtgatgttca acggcggggt cgatcccatg cgggagctgt ggagctcgct
     6061 gcgcgccgac ctgcgcggct gctcgatcct catgaccgag tacgtgagcc gcgacttctc
     6121 gctggtcgac gtgacatcgc ccgacgcgac caaggggcgc gcgctcgcct ggcgcgcgcg
     6181 ggagctcggc ctgactcgcg acgaggtcat ggccgtggga gacaacttca acgacgtcga
     6241 gatgctcgag ttcgccggcc atcccgtcgt gatggcgaac gcggtggccg agctgaagtc
     6301 acggggctgg cacgtgaccg gccatcagga cgagg
//
LOCUS       DBNGSH010000090         1656 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4251261, whole genome shotgun sequence.
ACCESSION   DBNGSH010000090 DBNGSH010000000
VERSION     DBNGSH010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4251261"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16CY_02055"
     CDS             complement(<1..461)
                     /locus_tag="AC16CY_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HIR3352587.1"
                     /translation="MREPPFAKASGAPGQEQFLQVLDRDEAERRFRAAIDLAPRGVEQ
                     VALDAALGRVLAADVVSPVDVPSFDRSNVDGFALVAEDTFGASEEVPRRVCLAGEVIH
                     TSVVPATVVRSGEAVAIATGGMMPRGADAVVMVEHGDVTEEGGKGAEGKGAG"
     gene            complement(454..>1656)
                     /locus_tag="AC16CY_02060"
     CDS             complement(454..>1656)
                     /locus_tag="AC16CY_02060"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3352588.1"
                     /translation="QALSWLDSQLHRLDAEDVPLRAAAGRVLAGAIVSDVDVPGFDRA
                     TMDGYALVAESTEGATPYNRMTLTVVGDSLPGAPFAGTVSRGEAVRIMTGAPIPAGAD
                     SVLPAESVENAVERGLEPPRTRQDIAAIAEVSPGKNVGRRAEDIAAGTTVLDAGRVLR
                     PQDVGVMSSIGLPRASVVRQPRVRLAITGNEVLPPGTTPRGNCITDSNGPMLTALAER
                     DGAIVDVRDPIRDDPDAILAALQADADIVIVSGGSSVGIEDLAPSLVAGHGELAVHGI
                     AMRPSSPTGMGRLGRRLVFLLPGNPVSALCAYDFFAGRAIRALGGRTKDWPYRSVRAT
                     LDRKISSPIGRLDYARVRVANGLVTPLSVAGASVLSSTTRADGFVIVDDDSEGFAAGS
                     EVDVWLYA"
BASE COUNT          252 a          609 c          560 g          235 t
ORIGIN      
        1 cctgccccct tgccctctgc ccccttgccc ccttcttcag tgacatcccc gtgctccacc
       61 atgacgaccg cgtcggcgcc gcgcggcatc atgcctcccg tcgcgatggc cacggcctca
      121 cccgatcgca cgacggttgc cggcacgacg ctggtgtgga tcacctcgcc ggcgagacag
      181 acgcgccgcg ggacttcttc ggatgcgccg aaggtgtctt ccgcgacgag ggcaaagccg
      241 tccacgttcg agcgatcgaa ggacggcacg tccacgggag agacgacgtc ggcggcgagg
      301 acgcggccga gcgccgcgtc gagtgcgacc tgctcgacgc ctcgaggagc gagatcgatc
      361 gccgcgcgga agcggcgctc cgcctcgtcc cggtcgagga cctgaaggaa ttgctcttgc
      421 cccggcgcgc ccgaggcttt agcgaagggc ggctcacgca tacagccaga cgtccacctc
      481 ggagcccgcg gcgaacccct cgctgtcgtc gtccacgatg acaaagccgt cggccctcgt
      541 cgtcgacgag agcaccgagg cgcccgcgac cgacaacggc gtcaccaggc cgttcgcgac
      601 tcgcacgcgc gcgtagtcca gccggccgat cggcgagctg atcttgcgat cgagcgtggc
      661 gcgcaccgat cggtacggcc agtccttcgt tcggccgccc agcgcgcgga tcgctcgtcc
      721 ggcgaagaag tcgtacgcgc agagcgccga caccggattg cccgggagga gaaagaccag
      781 ccggcgtccg agccggccca tgccggtcgg gctgctcggc cgcatcgcga taccgtggac
      841 ggccagctcg ccgtgaccgg ccacgagcga gggcgccaga tcctcgatcc ccacgctcga
      901 tccgccggac acgatgacga tgtcggcatc ggcctgcagt gcggcgagga tggcgtcagg
      961 atcgtccctg atcggatcgc gcacgtccac gatcgcgccg tcacgctcag cgagtgcggt
     1021 gagcatgggt ccgttggaat cggtgatgca attgccgcgc ggcgtcgtgc ccggcggcag
     1081 gacctcgttg ccggtgatgg ccagcctgac gcggggctgg cgcacgacgc tggcccgcgg
     1141 gagtccgatc gagctcatca cgccgacatc ctgcgggcga agcacgcgcc ccgcatcgag
     1201 caccgtcgtg cccgccgcga tgtcttccgc gcgacggccg acgttcttgc ccggcgagac
     1261 ctccgctatt gcggcaatgt cttgacgtgt ccgcgggggc tcaagccccc gctctacagc
     1321 gttctccacg gactcggccg gcagcaccga gtcagcgccc gccgggatcg gcgcgccggt
     1381 catgatgcgc accgcctcgc cgcgactcac ggtgccggcg aacggtgccc ccggcaacga
     1441 gtcgccgacg actgtcagcg tcatccggtt gtacggcgtc gctccctcgg tgctctcggc
     1501 gacgagcgca tacccgtcca tcgtcgcgcg atcgaagccg ggcacgtcca cgtcgctgac
     1561 gatggcgccg gcgagcaccc ggccggcagc ggcgcgaaga gggacgtctt cagcgtcgag
     1621 tcgatgcagc tgagagtcga gccacgacag cgcctg
//
LOCUS       DBNGSH010000091         5186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3572219, whole genome shotgun sequence.
ACCESSION   DBNGSH010000091 DBNGSH010000000
VERSION     DBNGSH010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3572219"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /gene="dnaJ"
                     /locus_tag="AC16CY_02065"
     CDS             complement(<1..1084)
                     /gene="dnaJ"
                     /locus_tag="AC16CY_02065"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3352589.1"
                     /translation="MSKRDYYEVLGVARTATDQEIKSAYRKLALQYHPDRNPGDKTSE
                     DKFKEAAEAYSVLVDTDKRHMYDRFGHAGLGGAATGGFDPNAFTGFEDILGGLGDIFG
                     FSDAFGRRRGGPQRGADLRYDLEISFEESAKGTEATLQIPRHETCETCSGNGSAPGSA
                     PSTCPQCQGRGQLRYQQGFFTVARTCGQCRGSGSIITKPCPTCRGAGRLQKDKKLSVR
                     IPAGIATGQRLRLNGEGEAGPGGGPAGDLYVVVHVQEHPFFQRDGNDLHCEMPLHFPT
                     LALGGEIRIPTLDGSETFTIPSGTETGTTFRVRGRGMPDVSGRGRGDLMVTVKVSTPK
                     KLTKEQKKLLEQLADVLPKEAYEPTPA"
     gene            complement(1081..1593)
                     /gene="grpE"
                     /locus_tag="AC16CY_02070"
     CDS             complement(1081..1593)
                     /gene="grpE"
                     /locus_tag="AC16CY_02070"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3352590.1"
                     /translation="MTEQTIDDQDQPQPEAQVESPADPLDELRREKDALQDRLLRTAA
                     EFDNYRKRMERERRELSDHIRADVLKDVLPIVDNFERALQAAGTETESFGKGVELIYR
                     QMLDFLKKRGVTPIEALGADFDPNFHQAVIHETSDAHREGEVMEELQRGYMLGDRLLR
                     PAMVKVAKTA"
     gene            complement(1621..2670)
                     /gene="hrcA"
                     /locus_tag="AC16CY_02075"
     CDS             complement(1621..2670)
                     /gene="hrcA"
                     /locus_tag="AC16CY_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR3352591.1"
                     /translation="MVSFELSDRSRRLLATLVRQYIDSGEPVSSQRVARESGLGVSSQ
                     TVRNALAQLEEHGFLHQPHTSAGRVPTDLGYRAFVDQLLEGRRPARSPADVEQQLRQQ
                     AGRTPLMDDLLASVSHLVSRAARHVGFALAADDGAVLQRIEFVPLDGSRVLVVVVSRG
                     NQITQKVVDAGEWVRPDDLAQAARYLNTEFAGLPLLDVREAVLERLRQERTLYDRLLA
                     RALTLARSTLEDLPHVQLFHVEGAASLLEHEGQDGVSTATLRALLEMMEEKERMIHLL
                     NAYIDGPGLTVVIGTEHTSPNLRRFSLIACTAVDGSTTRTVGVIGPTRMHYSRAIALV
                     DGTTQAVSRVLRQAH"
     gene            complement(2712..3545)
                     /locus_tag="AC16CY_02080"
     CDS             complement(2712..3545)
                     /locus_tag="AC16CY_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3352592.1"
                     /translation="MNQEVFAALSEALDRGEEVALVTIVAARGSTPQRVGAKMLVYSD
                     GRTVGTIGGGCYENDAFGKAREAIASRRPLNVKYELNDDFAQETGLVCGGQMEVFIEP
                     VESSPEVYVFGAGHVGYFVARLAHEAGFRVHVVDDREKFANAQRFGDGIEVIVDNIPA
                     WLQSHALPPTAYAVIVTRGHTHDLDALRALSAVPLRYLGLIGSKAKVRRIFDALIEEG
                     TSEESLRAVHAPIGLDIGAITPQEIAVSIVAELIAAKHGKINDPRVAAVSLRWEGALR
                     E"
     gene            3763..3921
                     /locus_tag="AC16CY_02085"
     CDS             3763..3921
                     /locus_tag="AC16CY_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352593.1"
                     /translation="MLLLIAGVAALVGAKLLSELQSRNVSWGRRALYGVALLMIFGAF
                     SKNWRRGG"
     gene            complement(3924..>5186)
                     /gene="bshC"
                     /locus_tag="AC16CY_02090"
     CDS             complement(3924..>5186)
                     /gene="bshC"
                     /locus_tag="AC16CY_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HIR3352594.1"
                     /translation="RVEHHVPAVAVFWVDAEDHDWNEVKACGVLDADLRLQSISPGDP
                     QGANHGPVARVRLDEHITATLSQLEATLPATEFTPGLLEDLRRIYRPGAGMADAFSQW
                     LERVLGSRGLVVFDASDPAAKPMAAELFALEVERAGATSRLAAEAGEALVLRDYHAQV
                     IPQEDGLALFAIDDQREPIRKAGDRFAIGSRTESANDLLQRVRLSPESFSPNVLLRPL
                     VQDTIFPTVCYVAGPNELAYLAQLRRVYEAFSVPMPLVQQRSTATIVDGNAMRFLSRP
                     DVRFESLRAQDEAALNELLVAQLPPSVDASIDEARSALDQRMEAVAREAAGVDATLEG
                     ASRSTLGRMQDDLKKLQAKIVQAAKRKDDTLRRQFRHAQDQAFPAGQPQERAVGFVYF
                     LNRYGPGLVDRLMEVLPGEMGKHEIVTI"
BASE COUNT          784 a         1779 c         1703 g          920 t
ORIGIN      
        1 cggcgggtgt cggctcgtag gcttccttcg gaaggacgtc cgccagctgt tcgagcagct
       61 tcttctgctc cttggtcagc ttcttcggcg tgctgacctt gacggtgacc atcaggtcgc
      121 cgcgtccgcg cccgctgacg tccggcatcc ctcgtccacg cacgcggaac gtcgtcccgg
      181 tctcggttcc cgaaggaatc gtgaacgtct cgctgccgtc gagcgtcggg atcctgatct
      241 ctccgccgag cgcgagggtg gggaagtgaa gcggcatctc gcagtgaagg tcgttgccat
      301 cgcgctggaa gaacggatgt tcctgcacgt gcacgacgac gtagaggtcg ccagccggcc
      361 cgccgccggg accggcttcg ccctcgccgt tcagcctgag ccgctgaccg gttgcaatgc
      421 cggccggaat ccgcacgctg agcttcttgt ccttctgcag gcgcccggcg ccgcggcatg
      481 tcgggcatgg cttggtgatg atggagccgc tgccccgaca ctggccgcag gtcctggcca
      541 ccgtgaagaa gccttgctgg tatcgaagct gcccacggcc ctggcactgc ggacaggtgg
      601 acggcgccga gcccggcgcc gagccattgc ccgagcaggt ctcacacgtc tcatgccgcg
      661 gaatctggag cgtcgcctcc gtgcctttcg ccgactcctc gaacgagatc tccaggtcgt
      721 agcggaggtc cgcgccccgt tgcggcccgc cgcgccgcct cccaaaggcg tcgctgaagc
      781 cgaagatgtc gccgagtccg ccgaggatgt cctcgaagcc cgtgaacgcg ttgggatcga
      841 agccgcccgt ggcggcgcct ccgagtcccg cgtggccgaa gcggtcgtac atgtgccgct
      901 tgtcggtgtc caccagcacg gagtacgcct ccgccgcttc cttgaacttg tcctcggacg
      961 tcttgtcgcc gggattgcga tcggggtggt actgcagcgc gagcttgcgg tacgcgctct
     1021 tgatctcctg gtcggtcgcg gtgcgcgcga cacccagcac ctcgtagtag tctcgcttgc
     1081 tcacgcggtc ttggccacct tcaccatggc gggtctcaga agccggtcgc cgagcatgta
     1141 cccccgctgc agctcctcca tgacctcgcc ctcgcggtgc gcgtcgctcg tctcgtggat
     1201 gacggcttga tggaagttcg ggtcgaagtc ggcgccgagc gcctcgatcg gcgtgacgcc
     1261 ccgcttcttg aggaagtcga gcatctgccg gtaaatcagc tcgacgccct tgccaaacga
     1321 ctcggtctcg gtgccggcgg cctgcagggc gcgctcgaag ttgtccacga tcggaagcac
     1381 gtccttcagc acgtcggccc ggatgtgatc ggagagctcg cgccgttccc gctccatgcg
     1441 cttgcggtag ttgtcgaact ccgcagcggt acgaagcagg cggtcctgca gcgcgtcctt
     1501 ctcgcgacgc agctcgtcga gcggatcggc cggtgactcg acctgggctt ccggctgtgg
     1561 ctgatcctga tcgtcgatcg tctgttctgt catcgaacga ctaatgcgaa gggcaagcgg
     1621 ctagtgagcc tggcgtagca ctcgcgacac ggcttgcgtg gtgccgtcca cgagcgcgat
     1681 ggccctggag tagtgcatcc gcgtcggacc gatgacgccg acggtgcgcg tcgtgcttcc
     1741 gtcaacagcc gtgcacgcga tcaggctgaa ccgcctgaga ttgggcgacg tatgctccgt
     1801 gccgatcacc acggtcagtc ccgggccatc gatgtacgcg ttgagcagat ggatcatccg
     1861 ctccttttcc tccatcatct ccagcagggc ccgcagcgtc gccgtcgaga cgccgtcctg
     1921 gccctcgtgc tcgagcagcg atgcggcgcc ctcgacgtgg aacagctgca cgtgcggcag
     1981 atcctccagc gtcgaccgcg cgagcgtcag cgcgcgcgcg agcagcctgt cgtacagcgt
     2041 gcgctcctga cggagccgct cgagcaccgc ctcgcgcacg tcgagcagcg gaaggccggc
     2101 gaactccgtg ttcagatacc gcgccgcctg ggccagatcg tcggggcgca cccactcgcc
     2161 ggcatcgacc actttctgcg tgatctgatt gccccgggag acgacgacca ccaggacccg
     2221 ggacccgtcg agcggaacga actcgatgcg ctgaagcacg gcgccgtcgt cggccgccag
     2281 ggcgaacccc acgtgccgcg ccgcgcgcga gaccaggtgc gacacgctgg caagcaggtc
     2341 gtccatgaga ggcgtccgcc cggcctgctg gcgcagttgc tgctcgacgt cggccggcga
     2401 tctcgcgggc ctgcggccct cgagcagctg gtccacgaac gcgcgatagc cgaggtcggt
     2461 cggcacgcgg cctgcggacg tgtggggctg gtggaggaat ccgtgctcct cgagctgagc
     2521 cagcgcgttg cgcaccgtct gcgacgacac ccccaggccg ctctcgcggg cgacgcgttg
     2581 cgacgagaca ggctcgccgc tgtcgatgta ctggcgcacg agtgtcgcca ggaggcggcg
     2641 cgatcgatcg gagagctcga aggaaaccat tgccagtcag gggcttaggc gcttgcagga
     2701 aattatagca cctactctcg gagcgccccc tcccaacgca gactgacggc ggcgacgcga
     2761 gggtcgttga tcttgccgtg cttggcggcg atcaactcgg ccacgatgct gacggcgatt
     2821 tcctgcggcg tgatcgcgcc gatgtcgagt cctatcggcg cgtggaccgc gcggagcgac
     2881 tcctcagagg tgccctcttc gatgagcgcg tcgaagatcc gccgcacttt cgccttgctg
     2941 ccgatgagac cgagatagcg aagaggcacc gccgacaggg cacgcagcgc gtcgaggtcg
     3001 tgcgtatggc cgcgtgtcac gatgacggcg tacgcggtcg gcggcagcgc gtgcgactgc
     3061 agccaggccg ggatgttgtc gacgatcacc tcgatcccgt ccccgaatct ctgggcgttg
     3121 gcgaactttt cccggtcgtc caccacgtgt actcggaacc cggcttcatg ggccagcctg
     3181 gcgacgaagt accccacgtg cccggccccg aagacgtaga cctctggcga cgattccacg
     3241 ggctcgatga acacttccat ctggccaccg cagacgaggc ccgtctcctg ggcgaagtcg
     3301 tcgttgagct cgtacttgac gttcagcggt cgccgggacg cgatggcttc ccgcgccttg
     3361 ccgaacgcgt cgttctcgta gcagcccccg ccgatggtcc cgacggtgcg gccgtcgctg
     3421 tagacgagca tcttcgcgcc cacgcgctgg ggcgtcgagc cccgcgcggc gacgatggtg
     3481 acgagcgcca cctcttcgcc gcgatcgagc gcctcggaca gggcggcgaa aacctcctga
     3541 ttcattcaga taaattatag agcctcgacc cgggctccgc cgccctgcgc gaacttttgg
     3601 ggcactctac tcgtcgttac aagcgtgttc cgcccccggt accgtcgcca gccgtttgcc
     3661 gacccactca actggaccga ttttcgctgg gattccaacc cgtggcacac ggcggcggcc
     3721 gccccgcgcg gatttggaca ccggcgccgc cggcacccgt tggtgctgct tctcatcgcg
     3781 ggggtcgcag ccctggtcgg tgccaagctc ctgtcggagc tccagtcgcg gaacgtctcc
     3841 tggggtcgcc gtgccctcta tggcgtcgcg ctgctgatga tcttcggcgc cttttccaag
     3901 aactggcgtc ggggaggctg acactagatc gtgacgatct cgtgctttcc catctcgccc
     3961 ggtaacactt ccatcaagcg gtccaccagt cccggaccgt acctgttgag aaagtagacg
     4021 aagccgaccg cccgttcctg gggctgcccc gcgggaaacg cctggtcctg agcgtgtcga
     4081 aactgcctgc gcagcgtatc gtccttccgc ttcgctgcct ggacgatctt ggcctgcagt
     4141 ttcttcaggt cgtcctgcat ccgtccgagc gtcgaacggc tcgccccctc gagcgtggca
     4201 tcgaccccgg cggcctcccg agccactgcc tccatccgtt ggtcgagagc ggacctggct
     4261 tcgtcgatcg acgcgtcaac cgacggcggc agctgtgcga cgagcagttc gttcagcgcg
     4321 gcctcgtcct gcgcgcggag cgactcgaat cggacgtctg gccgcgacag gaaccgcatc
     4381 gcgtttccat cgacgatcgt ggccgtggaa cgctgctgga ccaggggcat cgggaccgag
     4441 aacgcttcgt agacccggcg cagttgtgcg aggtaggcga gctcgttggg cccggccacg
     4501 tagcacaccg tgggaaagat cgtgtcctgc acgagtggcc gcagcaggac gttcgggctg
     4561 aatgactccg gcgagagacg tacgcgctgg agcagatcgt tcgccgattc ggtccggctc
     4621 ccaatcgcga accggtcgcc ggctttccga atgggctctc gctgatcgtc aatcgcaaac
     4681 agcgcgaggc cgtcttcctg cggaatgacc tgggcgtgat agtcgcgcag gaccagtgcc
     4741 tcgcctgcct ccgcggccag tcgtgacgtg gcccccgcgc gttcgacctc gagggcgaag
     4801 agctccgccg ccatgggctt ggccgccgga tccgaggcgt cgaacaccac aaggccgcgc
     4861 gatccgagga cgcgctcgag ccactggctg aaggcgtctg ccatgccggc gccggggcgg
     4921 tagatcctcc gcagatcctc gagcagtccc ggcgtgaact ccgtggctgg aagcgtggcc
     4981 tcgagttgag acagcgttgc cgtaatgtgt tcgtcgagac ggacccttgc gaccgggccg
     5041 tgattggccc cctgcggatc tcccggcgag atcgactgaa ggcggagatc ggcgtcaagc
     5101 acaccgcagg ccttcacctc gttccagtcg tgatcctcgg cgtccaccca gaacacggcc
     5161 accgcgggca cgtggtgctc gacccg
//
LOCUS       DBNGSH010000092         4643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3741999, whole genome shotgun sequence.
ACCESSION   DBNGSH010000092 DBNGSH010000000
VERSION     DBNGSH010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3741999"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="AC16CY_02095"
     CDS             complement(<1..821)
                     /locus_tag="AC16CY_02095"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3352595.1"
                     /translation="MTSLPDGRLAFVEGEHRVRIIASRALVPEPALAVDPSHARIVGL
                     VADPNFIATRMVFVSWIERRASGESVLNVTRYREVQNEFGEGATIVTGIPVPERAVVP
                     LAADPAGRLYVAIPASASGGAGHVARFGFDGSVPRDGLPFSPVFAEGYASPTGLAVTP
                     GANQLWLGGNSSGDSEALSSISLQTNAASQWPLRRRAVRQGQFGDLSFVSLTRPAASA
                     NSSQNPAASTVLVGAGRRLFVGLVSAAGIASGFDQVRFDPDVQVQAAAPAPDGSI"
     gene            complement(1495..1914)
                     /locus_tag="AC16CY_02100"
     CDS             complement(1495..1914)
                     /locus_tag="AC16CY_02100"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR3352596.1"
                     /translation="MRLPDEGKVAGVCAGLAAYFEVDVTVVRLAWVILSIIPGVFIGG
                     LVAYAAAWILTPVAASQSQGVPGKHLTRSVADKQIAGVCGGLAEYFGLDATIVRLAAV
                     ILAVYPGAVIGGILAYLIAWVIMPAGPVASLQPASSS"
     gene            2228..3163
                     /locus_tag="AC16CY_02105"
     CDS             2228..3163
                     /locus_tag="AC16CY_02105"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR3352597.1"
                     /translation="MRVLITGAAGFLGSHLCDRFLREGHSVVGLDNLLTGRPGNIAHL
                     NGHGRFEFIRHDVSTYTDVPGQLDGILHFASPASPIDYLEHPIATLKVGALGTHNALG
                     VARAKGARFLLASTSEVYGDPLVHPQPESYWGNVNPIGPRSVYDEAKRFAEAMTMAYH
                     RVHGVDTRIVRIFNTYGPRMRPNDGRVVSNFIVQALTGRPLTVYGDGRQTRSFCFVDD
                     EIDGIYRLFLSSETSPVNIGNPNEFTIEELAQTVLELIGSRSSVERRDLPEDDPRVRQ
                     PDITRARTLLGWNPKVDLREGVKRTIDYFRQELAR"
     gene            3314..>4643
                     /locus_tag="AC16CY_02110"
     CDS             3314..>4643
                     /locus_tag="AC16CY_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3352598.1"
                     /translation="MRRPDHWRWPLLTLALLAVLAFGPACAGDPEARKQTYLASGDGY
                     FDKGDYSTAIIEYRNAIAIDARFGKAHAKLAESYLRTGDAANALIAFARAADLLPDDF
                     DVNLKTGVLLLAAGRPEDALGRAEVALKLRPQDVTAHVLRGNALAGLKSFDEALKAIE
                     EAIRLDPERGTTITQKAFIELASGRREEAERTFKRAVEIAPNAVEGYLALGNYYWALG
                     RLTETEQTFRSALKVKPHDPSANRAMAALSITTGRTAEAEQYLRTIADTFGDSASVVA
                     LANYYLLAGRSKDAIDKLTPLATGKEKVPGTPQLLARAYALSGDSGKARELVDTILEA
                     ERGDLDAQLLKAQLLYQDGKRDAALALVAAAASANPTSVEAQLMLGKLHVARGDVAPA
                     EEALREVLRLNPRVTAAQVELSRLLLSTGRTADSLSNAEEAARNEPRSPEA"
BASE COUNT          885 a         1540 c         1487 g          731 t
ORIGIN      
        1 tgaatcgagc cgtcaggggc cggcgctgct gcctggacct gcacgtcggg atcgaagcga
       61 acctggtcga agcccgacgc gattccagcc gctgaaacga gaccgacaaa cagacgtcgg
      121 cctgcaccaa cgagaaccgt tgaagcggcc gggttctgac tcgagttcgc ggacgcagct
      181 ggcctcgtga gcgagacgaa agacagatcg ccgaactgcc cctgccgaac agcgcgtcgc
      241 cgcagaggcc actgactggc cgcattcgtc tgcagcgata tcgaggacag cgcctcgctg
      301 tctcccgagc tgttccctcc taaccacaac tgattcgcgc cgggcgtgac ggccagccca
      361 gttggcgagg cgtagccttc cgcaaatacg ggggagaatg gaaggccgtc tcgcggcacc
      421 gatccgtcga acccgaagcg cgcgacgtgt ccggcgcctc cgctcgcact cgctggaata
      481 gccacgtaca agcggccggc gggatcggca gcgagcggaa cgacggcacg ctcgggcacc
      541 gggattcccg tgacgatggt cgcgccctcc ccgaactcgt tctgaacctc gcgatagcgc
      601 gtcacgttca agaccgattc gcctgacgct cgtcgttcga tccacgaaac gaacaccatt
      661 cgcgtggcga tgaagttggg gtccgccacc agcccgacga tgcgcgcgtg tgacgggtcc
      721 acggcgagtg ccggctcggg cacgagcgca cgggaagcga tgatacgaac ccggtgttcg
      781 ccctcgacga acgccagtcg gccatccggc aacgatgtca tggccgcggc actgtcgata
      841 ccgctcgcga cgacgcgcac gtcatagcag ggcgcgggcg atcctgacga gaggcacgcc
      901 gtcacgagtg ttggcgagtt caagccgcct ctcggcacgt cagtgccgcc gcccccaccg
      961 ccgccacccc ccccgccgcc gccaccacca tcgccacccc cacccccgcc cccgcctcct
     1021 gaggtctggt tgactctcag cggagccgaa cgcggactct ccacgccgtt gagaacagag
     1081 gtgagttgca aggtgtgcgg atccgagctc atcgaaggca ggacgcccga gcactcgtat
     1141 agtccgccgt tggcctcgtc ggaacaacgt gtccccccga gtgcggatcc ggcgttgtcg
     1201 acgtagatcc tgaaggagag actcctgagt tcctggaggg agcctgctga ctggtcccac
     1261 gcgaggcgct cgccgccacg aactgtctgg ctattctcct cgctgcaggc aaggccgaag
     1321 acgagcagag ggcaaagcag aacgactcgg agcgtggacg ccgagttcac tccgcccgaa
     1381 gacacggcga gtgtcatcaa cgcgatgata tacggtcttc atcggcgccg gccggcccgt
     1441 gtcaaactgt gccgtatggc gtaattcaat gaagcaaaca cgccctcttt tgcttcagct
     1501 cgacgacgcg ggttgaagcg acgcgacggg gcccgcaggc atgatgaccc acgcgatgag
     1561 ataggccagg atcccgccga tgacggcgcc tggatacacg gccaggatca ctgcggccag
     1621 gcggacgatt gttgcatcca gtccaaagta ctcggcgagc ccgccacaga cgccggcaat
     1681 ctgcttgtcg gcgacagacc gggtcaggtg cttgcccggg acaccctggc tctgcgacgc
     1741 tgcaacaggt gtaaggatcc aggcggctgc gtacgccacg agtccgccga tgaacacgcc
     1801 cgggatgatt gacaggatta cccaggccag gcggaccacc gttacatcga cctcgaaata
     1861 ggcggccagg ccggcgcaca cgccggcgac cttgccctcg tccgggaggc gcacgaatcg
     1921 gcgggctgga tccttgggcc tgaccgaggc gccgcagaag cggcaatacg acgactcagc
     1981 ctcgttctca cgttggcatt tggcgcagac cacggcacac cccctcttaa ggctctctga
     2041 tgtgtgatac gcgaatacag ggcaggaagg ttcgcgcagg caccggtacg ccacttgcca
     2101 cgaacgccgt ctgactccag acttgtcacc cgaggattcg cgtgcagatc tcagttgtcg
     2161 cagcgggata cgtgggccag ggccggatcg cggcaagaat gccatcagcc atcggacggg
     2221 gcggccgatg agagtgctga tcaccggcgc ggccggcttt ctcggttctc atctgtgcga
     2281 tcggtttctt cgcgaagggc attccgtcgt cggactggac aacctcctta cgggccgtcc
     2341 agggaacatc gcgcacctga acggacacgg gcgattcgag ttcatccggc acgacgtctc
     2401 cacctacacg gacgttcccg gtcagctgga tggaatcctg cacttcgcgt cgccggcatc
     2461 cccgatcgac tacctcgagc atccgatcgc gacgctcaag gtcggcgcgc tcggtacgca
     2521 caatgcgctg ggcgtcgcca gggccaaagg cgcgaggttc cttctcgcat cgacgtcaga
     2581 agtgtacggc gatcccctcg tccaccctca gcccgagtcg tactggggga acgtcaatcc
     2641 catcggcccg cggagcgtct acgacgaagc caagcgcttc gccgaagcga tgacgatggc
     2701 gtaccaccgg gtgcacgggg tcgacacgcg catcgtccgc atcttcaata cgtacggacc
     2761 gcgcatgagg cccaacgatg gccgagtggt gtcgaacttc atcgttcagg cactgaccgg
     2821 ccggccgctg acggtctacg gcgacggcag gcagacccgc tcgttctgtt tcgtggacga
     2881 cgagatcgat ggcatctacc gtctgttcct gagctcggaa acgagccccg tcaacatcgg
     2941 gaatccgaac gagttcacga tcgaggagct cgcgcagacg gtactcgagc tgatcggatc
     3001 ccgcagcagt gtcgaacgcc gggacctccc cgaggacgac ccgcgagtcc ggcagccgga
     3061 tatcacacgc gcgaggactc tgctcggatg gaaccccaag gtggacctgc gcgaaggtgt
     3121 gaagcgcacg atcgattact tccggcagga gcttgcccgc tgaggagcga ctgtgggcgg
     3181 ccggagaaag atccctcgtc aggtggactt ttggccttcc ggttcgggct gtctttgtca
     3241 gaacccggca ccctcattgc accccgccac gtggcagtat tctgggacca ccacacatcg
     3301 agttagctca cttatgcgaa gacctgatca ctggcgttgg ccgctcctga ctctcgccct
     3361 cctcgcggtt ctggcgttcg gtcccgcgtg cgccggcgat cccgaggcca ggaagcagac
     3421 gtatctggcc agcggtgacg ggtacttcga caagggcgac tacagcacgg ccatcatcga
     3481 gtaccgaaac gcgatcgcca tcgacgcccg atttggcaag gcgcacgcga agctggctga
     3541 gtcgtacctg cgaacggggg acgcggccaa cgcgttgatc gcgtttgcgc gcgcggccga
     3601 tcttctgccg gacgacttcg acgtcaacct caagacgggt gtgctgcttc tggcggccgg
     3661 gcggcccgag gatgccctgg gcagggccga ggtcgcactg aaactgcggc cgcaggacgt
     3721 gacggcacac gttctccgag gaaacgcgct ggcagggctc aagtcgttcg acgaagcgct
     3781 caaggcgatc gaagaggcga ttcggctcga cccggaacgt ggcacgacca tcacgcagaa
     3841 ggctttcatc gagctcgcca gcggacgccg cgaggaagcc gagaggacgt tcaagcgtgc
     3901 tgtcgagatc gcgcccaacg cggtggaggg ctacctcgcc ctcggcaatt actactgggc
     3961 gcttggccgc ctcaccgaaa ccgagcagac gtttcggagc gcgttgaagg tcaagccaca
     4021 cgacccgtcg gcgaatcgtg cgatggcggc cctttccatt acgacaggac gaaccgccga
     4081 ggccgaacaa tacctccgaa cgattgccga cacattcggc gattccgcgt cggtggtcgc
     4141 gcttgcgaac tactacctgc ttgccggccg gagcaaggac gcgatcgaca agctgacgcc
     4201 gctcgccacc ggcaaggaga aagttccggg aaccccgcag cttctggccc gcgcgtacgc
     4261 gctctcgggc gacagcggca aggcgcggga gctcgtggac acgattctcg aggccgagcg
     4321 cggggatctg gatgcgcagc tcctcaaggc gcagctcctg taccaggacg gcaagcgaga
     4381 tgcggctctc gcgctcgtgg cagcggcggc cagcgcgaac ccgacgtcgg tggaagcgca
     4441 gttgatgctc ggcaagcttc acgtggctcg cggcgatgtc gcgccggccg aggaggccct
     4501 tcgagaagtg ctcaggctca atccgcgcgt gacggcggcg caggttgagc tgtcgagact
     4561 gctgctgtcg acaggccgca ccgccgactc gctcagcaat gccgaagaag ccgcgcgcaa
     4621 cgagcctcgc agccccgagg ctc
//
LOCUS       DBNGSH010000093         4572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2044418, whole genome shotgun sequence.
ACCESSION   DBNGSH010000093 DBNGSH010000000
VERSION     DBNGSH010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2044418"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="AC16CY_02115"
     CDS             complement(<1..259)
                     /locus_tag="AC16CY_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352599.1"
                     /translation="MKSLIQAGALAAAVVVLCQSLAAQWDPHPIPGVPRTATGEPNMD
                     APVPRRADGKPDLSGVWQGGGGGFGRGQQAPAAPPGSPPVAG"
     gene            455..871
                     /locus_tag="AC16CY_02120"
     CDS             455..871
                     /locus_tag="AC16CY_02120"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3352600.1"
                     /translation="MSSRLLVLCAGLVVALGLRLEAHHAFAAEFDRDKPITLTGAVTK
                     LEWTNPHARIYIDVKDKDGKVVNWDFELGPPNGLMRRGWNRNSLRQGHVVTIEGFLSK
                     LAPNVANARTVKLPDGRQVFAGSSFDTGAAEGDPAK"
     gene            1086..3242
                     /locus_tag="AC16CY_02125"
     CDS             1086..3242
                     /locus_tag="AC16CY_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352601.1"
                     /translation="MQKDNAKTHVADVAAVEGTLQLLARAVRQFHTYPPTSPLCVDAI
                     KACHDALQSIQHRDRVVSRVTPSEFIVDEHQIGTGTVVEQELTRRLFKLRVAGLELEC
                     GATTRDLTRFCIDLVESDDHQGAEVTFAERLAEHGVESIVPVMAHRPVVMDIGVPPSH
                     TADLLAHERRRREHIAQPDAPVNYLYPPDKGWIRLDPGQHLGDVSLLDLVVLVEDPAD
                     IATMLARLTDDEASGPDARESALERKYSDVTRLFSSVEPRLAQVMFDKLARAVLKLEP
                     ERRNKLLQRTILPGLLDGRPDGQVLHAFPDMDLAEAICLLLDLETAAPEVISAALNRL
                     ELAPDRRAALVPLIEDRMRATVGDGDRARTGTAGIERYARELIRVQSARETDFSEFAT
                     FDLSMDGQARTAVSDVRGGIGTTDALMTQLLCVSQLVRIERNPGLVEAFMGRALDMIA
                     ALERSGRWRDVVSTVKGFGLLCGELRARRPDVADVMTKSLREYCTSTRLLALADLHER
                     DAEGRATVEDLARALGAMLVPGVIALMNHGPHQAKARSFTPLLCELAPAVSAALVAQL
                     DSCGPAAARMVAKVLGHAGAGVEIHLGRLAEHADQQVARESLRALARIGTPTAAGLIG
                     RQIREGATEIQATAEDALWHVPPAQIATQVRELLRSREFVCAHPQTAARLMDRAAQMK
                     VSGLGEALTNIEKLRFRFWKPSLVQVALKARELRSR"
     gene            3239..>4572
                     /locus_tag="AC16CY_02130"
     CDS             3239..>4572
                     /locus_tag="AC16CY_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3352602.1"
                     /translation="MTAPKTSHAHDPLALFHGLASLRKLAGTYPAGHPMIAQKLKELG
                     DLTADLLKAGSPVRIDVIRGDVYLDGVSSSADKQANRQLIGQLSALGVDSIHIHDGVA
                     LDEFLAVAELLWHHGDSSEPIASQLAARHVRHINLGRLVPLDTRWRSQQWADAPTGPM
                     DPAYAEAIVRTQETWENTTAGKPLDAVTVRDIVQLLLYKVARSNAALGQILAVKQYEN
                     LTYCHSVNVAMLSLLLGKNVGLDEPTLAALVEAALLHDVGKTQIPLDIVRKPGALDKG
                     ERRTIESHTTLGAQILVQSEGLHPLTPTVALEHHRGVKGNGYPDLGDAIPHPMSQIVS
                     VADVYEAITGARTYQAPTPPERACLILARMAGDKLNTGLVKAFVNAITFFPVGSFVRT
                     NRNELGLVVQTNGGEPLHPVLTLIDERTYQPKGRVDTAERDSAGAYARHISQ"
BASE COUNT          770 a         1575 c         1535 g          692 t
ORIGIN      
        1 accccgcgac cggcggactg cccggcggcg cggccggcgc ctgttgtccc cggccaaacc
       61 cgccgccacc gccctgccac actccggaca gatcgggctt cccgtcggcc cttcggggaa
      121 caggggcgtc catgttgggt tctccggtgg ccgtccgcgg gacgccgggt atcggatgcg
      181 ggtcccactg agccgcgagc gactgacaga gaacgacgac ggcggccgcg agcgcgccgg
      241 cctgtatcaa cgacttcacc tcaacctccc gatgtctgaa ctcacctcag gcctcagggc
      301 cctggcctca ggcctcaggc tgtctcaaat ctgagcgcat cagggtatca tcctcgtcga
      361 acctccaact cccgacctcc aactcccaat cgttcctggt tttttagggg agttgggaat
      421 tgggagttgg gagttgattg cgaggagtga accgatgagc agccggcttc tcgtcctgtg
      481 tgcgggtctc gtggtggcac tcgggcttcg tctggaggcg caccatgcgt tcgcggcgga
      541 gttcgaccgc gacaagccga tcacgctcac gggggcggtg accaagctgg agtggaccaa
      601 tccgcacgcg cgcatctaca tcgacgtcaa ggacaaggac ggcaaggtcg tcaactggga
      661 cttcgagctg ggcccgccaa acgggctgat gcggcgcggc tggaaccgca actcgctccg
      721 ccagggacac gtggtcacca ttgaaggctt cctgtcgaag ctggcgccca acgtggccaa
      781 cgcgaggacg gtgaaactcc cggacggccg ccaggtgttc gccggctcct cgttcgatac
      841 gggagcggcc gagggcgatc ccgcgaagta gccgcgccta cgttgtccga gggccgtgcc
      901 cctcgcttcg gccagtcgat cggcgttctg gaataagcgc gcgcacgtaa ttgcaccgcg
      961 ctgcaccttc gcaccttcgc actctcgcac tctcgcaccg tgtcggcctc ccagtaaggg
     1021 tgtcttgcgc gggcgtgtcg gtcgataaca agaccaagag ttccacgccc agggggaccc
     1081 cgaagttgca gaaagacaac gcgaagacgc acgtggctga cgtggcggcg gtcgaaggta
     1141 cgctgcagct gctcgcacgg gcggtccgac aattccacac ctatccgccg acgagcccgt
     1201 tgtgcgttga cgcgatcaag gcgtgtcacg acgcgcttca atcgatccag caccgggacc
     1261 gcgtagtgtc gcgcgtcacc ccgtcggagt tcatcgtcga cgaacaccag atcggcaccg
     1321 gcaccgtcgt cgagcaggag ctgacgcggc gcctcttcaa gctccgcgtg gcggggctcg
     1381 agctcgaatg cggagccacc acgcgagatc tgacgcgctt ctgcatcgac ctcgtcgaat
     1441 ccgacgacca ccagggagcg gaggtgacct tcgccgagcg cctggccgaa catggcgtcg
     1501 agtccatcgt gccggtgatg gcgcaccggc cggtcgtcat ggacatcggc gtcccgccat
     1561 cgcacaccgc ggacctgctg gcccacgaac ggcgccggcg cgagcacatc gcacagcccg
     1621 acgctccagt caactatctc tacccgcccg acaagggctg gattcgtctg gatcccggac
     1681 agcacctggg ggacgtgtcg ctcctcgatc tcgtcgtgct cgtcgaggat cctgccgaca
     1741 tcgcgacgat gctcgcgcga ttgaccgacg acgaggcgag cggccccgac gccagggaat
     1801 cggcgctcga acggaagtac agcgacgtga cgcgcttgtt ctcgtccgtc gagcccaggc
     1861 tcgcgcaggt gatgttcgac aagctggcgc gcgccgtcct gaagctcgag ccggagcgcc
     1921 gcaacaagct gctgcagcgc acgatccttc cgggcctgct cgacggacgc cccgacggcc
     1981 aggtcctgca cgcctttccc gacatggacc tcgcggaagc gatctgcctt ctgctcgacc
     2041 tcgaaacggc ggcgccggaa gtgatctccg cggccctgaa tcgtctggag ctggcgccgg
     2101 atcgacgcgc ggcgctcgtg ccgctcatcg aggatcggat gcgcgcgacc gtcggcgacg
     2161 gcgaccgcgc acggaccggg acggccggta tcgagcggta cgcgcgcgag ctgattcgcg
     2221 tgcagtcggc gcgcgagacc gacttctccg aattcgccac cttcgacctg tcgatggacg
     2281 gtcaggccag gaccgccgtc tcggacgtcc gcggcggcat cggcacgacc gatgcgctga
     2341 tgacgcagct cctgtgcgtg tcgcagctgg tgcgcatcga gcgcaatccg ggcctggtgg
     2401 aggcgttcat gggccgggcg ctcgacatga tcgccgcact cgaacgcagc ggccggtggc
     2461 gcgacgtcgt ctccacggtg aaggggttcg gccttctctg cggagagctc agggcccgcc
     2521 gacccgatgt ggccgacgtc atgaccaagt ccctgcgcga gtactgcacc tcgacacggc
     2581 tgctggcgct ggccgacctc cacgaacggg acgccgaggg gcgcgcgacg gtcgaggatc
     2641 tcgccagggc gctcggagcc atgctggtac ccggcgtgat cgcgctgatg aaccacggcc
     2701 cgcatcaggc gaaggcgagg tccttcactc ccctgctgtg cgagctggct ccggccgtgt
     2761 cggcggcgct cgtggcgcaa ctcgactcgt gcggcccggc tgcagcgcgc atggtggcga
     2821 aggtccttgg acatgccggt gcgggcgtcg agattcacct gggccggctt gccgaacacg
     2881 ccgaccagca ggtggcgcgg gaatcgctcc gggcgctcgc caggatcggc acgccgacgg
     2941 cggcggggtt gatcgggcgg cagattcgcg agggggcgac cgagattcaa gcgaccgcgg
     3001 aggacgcgct ctggcacgtg ccgcccgccc agatcgccac gcaggttcgc gagctgctgc
     3061 ggtcgcgcga gttcgtgtgc gcccacccgc agacggccgc acggctcatg gatcgcgcgg
     3121 cgcagatgaa agtgtccggg ctgggcgagg cgctgaccaa catcgagaag cttcgcttcc
     3181 ggttctggaa gccgagcctc gtgcaggtgg cgttgaaagc gcgggagctg cgcagccgat
     3241 gaccgcgccg aagacctcgc acgcgcacga tccgctggcg ctcttccacg ggctcgccag
     3301 ccttcgaaaa ctggcgggca cctaccccgc cggtcacccg atgattgcgc agaagctcaa
     3361 ggagctcggc gatctgacgg ccgacctgct caaggccggc agccccgtgc ggatcgacgt
     3421 cattcgaggc gacgtgtacc tcgacggcgt ctcgtcgagc gccgacaagc aggcgaaccg
     3481 gcagctcatc ggccagctgt ccgcgctcgg cgtggacagc attcacatcc acgacggcgt
     3541 ggcactcgac gagttcctcg ccgtggcgga gctcctctgg caccacggcg attcgtccga
     3601 gccgatcgcc agccagctgg ccgcgcggca cgtgcgccac atcaacctcg ggaggctggt
     3661 gccgctcgac acgcgatggc gctcgcagca gtgggcggac gcgccgaccg gtccgatgga
     3721 cccggcctac gccgaagcga tcgtgcgcac gcaggagacg tgggagaaca cgacggcggg
     3781 aaagccgctc gacgcggtga ccgtgcgcga catcgtgcag ctgctgctct acaaggtcgc
     3841 acgcagcaac gccgcgctcg gccagatcct ggcggtcaag cagtacgaga acctcaccta
     3901 ctgccactcg gtcaacgtgg cgatgctgag cctgctgctc ggcaagaacg tcggtctcga
     3961 cgagccgacg ctggcagccc tcgtcgaggc cgcgctgctg cacgacgtcg gcaagacgca
     4021 gattccgctc gatatcgtca ggaagcccgg cgcgctggac aagggcgagc gccggacgat
     4081 cgagtcacat acgacgctgg gcgcccagat tctcgtgcag tcggaggggc tgcacccgct
     4141 gacgccgacc gtggcgctcg agcatcatcg aggcgtgaaa ggcaatggct atcccgatct
     4201 cggggatgcg attccccatc cgatgagcca gatcgtctcg gtcgccgacg tctacgaggc
     4261 gatcaccggc gcccggacct accaggcgcc gacgccgccc gaacgcgcct gcctgatcct
     4321 ggcgcggatg gccggcgaca agctcaatac cgggctcgtg aaagctttcg tcaacgccat
     4381 cacgttcttc cccgtcggct ccttcgtccg gacgaacagg aacgagctgg gcctggtcgt
     4441 ccagacaaac ggaggcgagc cgctgcaccc ggtgctgacc ctgatcgacg agcgcaccta
     4501 ccagccgaaa gggcgcgtgg acacggccga gcgcgactcg gccggcgcct atgcccggca
     4561 catcagccag ag
//
LOCUS       DBNGSH010000094         2220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3912005, whole genome shotgun sequence.
ACCESSION   DBNGSH010000094 DBNGSH010000000
VERSION     DBNGSH010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3912005"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1035)
                     /locus_tag="AC16CY_02135"
     CDS             complement(79..1035)
                     /locus_tag="AC16CY_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352603.1"
                     /translation="MNRRILALVLLLAASPAAAQDPDVRWDRVQGDGVHLRVLRSYNL
                     SEGETASEPIVVLGGSARINGHAEDDVVVIGGTLRLGPKAVVDGDVVTVGGEIEKDPG
                     AVVRGTIDEAAVRMPGIVFDPEWSGRGWTYLAVGGTVVRMFFFLVVATLLTVIAPGWI
                     GSMSRRPAASSGLLGLAVQICFVPALLVVVIALIVSIVGIPLLAAIPFVLAALGFVWI
                     AGFTGVAVRVGAALRGRSRAEADASVGDLLLGFMAIAAMTVGGHLLALGFGWFSPMTW
                     PIRAAGLTIEYVAWTIGLGAAISSLFAASRATPPPVPTLSVL"
     gene            1149..>2220
                     /locus_tag="AC16CY_02140"
     CDS             1149..>2220
                     /locus_tag="AC16CY_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3352604.1"
                     /translation="MDHFEFGGHIAWRPTREYVERSRLAAFITKHGLRGLDDLMERST
                     RDIEWFWRAVLDDLGIEFYQPYSQVLDLSRGIARPAWCVGGRMNIVHNCLDKWCAGPA
                     SDRAALHWEGEEGAVRTQTYGQLLAEVNRAAHGFRSLGVEPGDRVALFMPMCPELVAA
                     FFAVIKAGGIVLPLFSGYGADAVATRLQDAGVTLLVTADGFWRRGAAVKTKETADAAV
                     RAAPTVDRVVVVSRLGLDVQMGPRDVSWSHLLSDQPETFETERTGADDPLMIIYTSGT
                     TGRPKGAVHTHCGFPVKAAQDMVHAFDVHAGETMYWVSDMGWMMGPWEVLGMTLIGGT
                     IVLYDGALDFPSPDRLWAVVERH"
BASE COUNT          386 a          772 c          734 g          328 t
ORIGIN      
        1 gtctcctttc gattgaagca gcagaagacc cgcgtggtcg ggaccgccaa caccatcctg
       61 caggtcagag cgggcggctc acagaactga caacgtcggc accggcggcg gcgtcgcgcg
      121 cgacgccgca aacagactgc tgatcgccgc gcccaggccg atcgtccacg cgacgtattc
      181 gatcgtcagg ccagcggccc tgatcggcca ggtcatcggg ctgaaccagc cgaagcccag
      241 cgcgagcagg tggccgccaa ccgtcatcgc tgcgatggcc atgaagccga gcagcagatc
      301 gcctacagac gcgtccgcct cggcacgact ccggccgcga agcgcggcgc cgacgcggac
      361 cgccaccccg gtgaagcccg cgatccagac gaaccccagc gccgcgagga cgaacggtat
      421 cgccgcaagc agcggaatgc cgacgatcga cacgatcaac gcgatgacga cgacgaggag
      481 cgccggaacg aagcagatct gcacggccag ccccagaaga ccagagcttg ccgccgggcg
      541 ccgcgacatc gagccaatcc accccggcgc gatcactgtc agcagcgtgg ccacgacgag
      601 gaagaaaaac atcctgacga ccgtgccgcc caccgccaga tacgtccagc cccgcccgct
      661 ccattccggg tcgaacacga tgccgggcat gcggaccgcg gcttcgtcga tcgtgcctct
      721 caccaccgcg cccggatcct tttcgatctc gccgccgacg gtgacgacgt cgccatcgac
      781 tacggccttc ggcccgaggc ggagcgttcc gccgatgacg accacgtcgt cttcggcgtg
      841 gccgttgatc cgcgccgatc cgccgagcac gacgatcggt tcggaggccg tctccccctc
      901 tgacaggttg tagctgcgaa gaacacggag atggacgccg tcgccctgaa cgcggtccca
      961 ccggacgtcc gggtcctgag ccgccgccgg cgacgcggcg agcagcagga caagcgcaag
     1021 gatccgtcga ttcactctgc gtctcctcgt cggttcagac ggagggccag ggaatgaagt
     1081 tccagacccg gcgtttccgc ctcctgtggc ccttgggcat tggccgttga gtgagctata
     1141 acccgtgcat ggaccacttc gagttcggcg gccacattgc gtggcggccg acgcgggagt
     1201 acgtcgaacg cagccgtctt gcggccttca tcacgaagca cgggctgcgg ggcctcgacg
     1261 atctcatgga gcggtcgaca cgggacatcg agtggttctg gcgagcggtc ctcgacgatc
     1321 tcggcatcga gttctaccag ccgtacagcc aggtccttga cctctcacgc gggatcgccc
     1381 ggcccgcctg gtgcgtcggc gggcgcatga acatcgtcca caactgcctc gacaagtggt
     1441 gcgcgggacc ggcgtccgac cgcgccgccc tccattggga aggagaagag ggcgctgtac
     1501 gcacgcagac atacggccag ctcctggcag aggtcaaccg cgcggcgcac gggttccgct
     1561 cgctcggcgt cgagcctggc gatcgagtgg cgctcttcat gccaatgtgc ccggaactgg
     1621 tggccgcctt cttcgcggtc atcaaggcgg gcggcatcgt actgccgctg ttctccgggt
     1681 acggcgccga cgcggtcgcg acgcgattgc aggatgcggg cgtcacgctt ctggtcacgg
     1741 ccgacggctt ctggcggcgc ggtgccgccg taaagacgaa ggagacggcc gatgcagcgg
     1801 tccgggccgc gcccacggtg gatcgcgtcg tcgtggtctc gcgcctggga ctcgacgtcc
     1861 agatggggcc gcgcgacgta tcgtggtcgc acctgctgtc cgaccagccc gagacgttcg
     1921 agaccgagcg caccggcgct gacgatccgc tgatgatcat ctacacctcg gggacgacag
     1981 gtcgtccgaa gggcgccgtc cacacgcact gcggcttccc cgtcaaggcg gcacaggaca
     2041 tggtgcacgc cttcgacgtc cacgccgggg agacgatgta ctgggtgagc gacatgggct
     2101 ggatgatggg cccgtgggaa gtgctcggca tgacgctcat cgggggaacg atcgtcctct
     2161 acgacggcgc gctcgacttc ccgtcgcccg atcgcctttg ggccgtcgtc gaacgccacc
//
LOCUS       DBNGSH010000095         4983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3232579, whole genome shotgun sequence.
ACCESSION   DBNGSH010000095 DBNGSH010000000
VERSION     DBNGSH010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3232579"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="AC16CY_02145"
     CDS             <1..1171
                     /locus_tag="AC16CY_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR3352605.1"
                     /translation="MARLTGSLYTPDETFRTDVATMLRLTAVQVSLGDDRAPRQGGAP
                     DIVIVDGRTDLDGAMHAVERLRASHPMATIFFMADESSPELILRAMRAGANEFFAWPP
                     SRAALDEALQRAASRRAQAPSGQDSKTIVFFGAKGGAGTTTVAVNCGVDIARLKKRPT
                     LIIDLKPGLGEVTLFLGVRSRYTLLDALENIHRLDSEFMRELVVKHKSGLEILAGSDV
                     FDRPGSNDTNAVEEVFRILGRQYEYIIVDAGAQPSACAFSSLYTADTICLVGNPDVPS
                     VRNAQRLLDRIGQLGTSSERVRLVLNRAAEPYPIPPAQIEKAVGHQIFQMFLSDYKTV
                     STALNSGVPIALAGNSELASQFDEFARRILDPSGAASEDSPKRGPLGLQRLASMW"
     gene            1178..2530
                     /locus_tag="AC16CY_02150"
     CDS             1178..2530
                     /locus_tag="AC16CY_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR3352606.1"
                     /translation="MRSAAPTLPPPATPARQAAAQAHQPVRQQYLELRAAVHRKLLGR
                     LNLEALAHVERAKAEGEIRSVLAQLLSEESTPISLAERETLFGEVLDDVFGLGPLEPL
                     LRDPSISDVLVNTYKNVFVERKGVLERVTATFQDDSHLLRVIDRIVSRVGRRVDDSSP
                     MVDARLADGSRVNAIIPPLAVDGPLLSIRRFPAERLKAEDLVNLGALTKPMLEFLAHC
                     VRAHLNCIISGGTGAGKTTLLNILSSYISARERVVTIEDAAELQLHQEHVVRLETRPP
                     NVEGKGAIRQRQLVVNALRMRPDRIVVGEVRSEEALDMLQAMNTGHDGSLTTVHANTP
                     RDALSRIETMIAMGATNLPDKAMRQQIASAIQLVVQQTRLADGTRKITSISEITGMEG
                     DVITMQEIFVFEKAGVSKEGKVIGRFRATGVRPKCSERLKSAGILLPTEMFEGVTEVR
                     "
     gene            2530..3486
                     /locus_tag="AC16CY_02155"
     CDS             2530..3486
                     /locus_tag="AC16CY_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3352607.1"
                     /translation="MLLAAIVFLVVTAIVAAVAFGVLRLPGSLAARRLEKRLSEVSNR
                     TEDDDKPASFIRSHAAGPLPALDRAFGRTGALAMLIEQSGTKTSPSSVVLASVALAVA
                     AAIASLLFIRQPFAWAIAALVGAAVPTFWLKYKRSARLKRFEEQFPEALDLLSRAIRA
                     GHAFQTAMGMCADELPAPVGVEFKKSFDQQNFGLPLKEALNELSERVPILDVRFFVTA
                     VTIQRETGGNLAEILDNLAHVVRERFKILRQVRVHTAHGRFTGYVLLSLPAALAVALS
                     YINPGHMDLLFKEHMGQMMILTAIVMQTIGYVWIRKVIKIEV"
     gene            3489..4391
                     /locus_tag="AC16CY_02160"
     CDS             3489..4391
                     /locus_tag="AC16CY_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3352608.1"
                     /translation="MTFLLPVLAFAFVALLIAGAAMALSPSGSMLIEQRLGELRGTPV
                     DKSVSYERVAKTLKHIGKYAPQSPSELGKLQKRLVCAGYRGKEALPVFIGIRLGSALL
                     AFGVASSPLVVRPNIFMALSVAAVAYLLPSMVLGRLAKKRQHRIRLSLADTLDLLVVS
                     VEAGLGLDQALQRVGEELAFAHPDLSDELRLINLELRAGKPRSEALRNLADRTGVDDL
                     SSLAAMLIQTDKFGTSVAQSLRVHSDTLRTKRRQRAEEAAAKTGVKMVFPLVFCIFPA
                     IWVVTIGPAAIKFVQVLFPLAQQQ"
     gene            4388..>4983
                     /locus_tag="AC16CY_02165"
     CDS             4388..>4983
                     /locus_tag="AC16CY_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR3352609.1"
                     /translation="MTTVLDDVRLAPPPAVPSTLEDTGLGTDQIEQLLIKTLYGGEAT
                     GLGLADRLRLTYTIFERVVERIRAERLIEVRGTVGSSASPNYRYALTDLGRDRARQYL
                     DINQYTGAAPVPLATYVAQMRALAASRGYIERERLRNGFSHLIVGDDILEQLGPAANA
                     NKAIFLYGPPGNGKTVLAEGLGRAIGGDMYMPYAIEVEG"
BASE COUNT          821 a         1718 c         1637 g          807 t
ORIGIN      
        1 catggcccga ctcacaggct ccctctacac ccccgacgag acgtttcgaa ccgacgtcgc
       61 cacgatgctg cggctcaccg cggtgcaggt gtcgctcggc gacgatcgag cgccgcgcca
      121 gggcggcgcg cccgacatcg tgatcgtcga cggccgcacc gatctcgacg gcgcgatgca
      181 cgcggtcgag cgccttcggg cgtcgcaccc gatggccacg atcttcttca tggccgatga
      241 gtcgagcccc gagctcatcc tgcgcgcgat gcgcgccggt gccaacgagt tctttgcctg
      301 gcccccgtcg cgcgccgcgc tcgacgaggc gctgcagcgc gcggcctccc ggcgcgcgca
      361 ggcgccctcg ggacaggact cgaagaccat cgtcttcttc ggagccaagg ggggcgccgg
      421 cacgacgacc gtcgccgtca actgcggggt cgacatcgcc cggctgaaga agcggccgac
      481 gctcatcatc gatctcaagc ccgggctggg cgaagtcacg ctgttcctcg gcgtgcgcag
      541 ccgctacacg ctgctcgatg cgctggagaa catccatcgc ctggacagcg agttcatgcg
      601 cgagctcgtc gtcaagcaca agtccggcct cgagattctg gccggatcgg acgtgttcga
      661 tcggcccggc agcaacgata ccaacgccgt cgaagaggtg ttcaggattc tgggccggca
      721 gtacgaatac atcatcgtcg acgccggtgc ccagccgagc gcgtgcgcgt tctcgtcgct
      781 ctacacggcg gacaccatct gtctggtggg gaaccccgac gtgccgtccg tgcgcaacgc
      841 ccagcggctc ctcgatcgca tcggccagct cgggacctcg agcgagcgcg tgcgcctcgt
      901 cctgaaccgc gcggccgagc cgtacccgat tccgccggcg cagatcgaaa aggccgtcgg
      961 ccatcagatc ttccagatgt tcctgagcga ctacaagacg gtgtcgacgg cgctcaattc
     1021 cggggtgccg attgcgctcg ccgggaacag cgagctggcg tcgcagttcg acgagttcgc
     1081 aagacggatc ctggatccct cgggcgccgc gtccgaggac tctcccaagc gcgggccact
     1141 cgggctgcag cgcctggcct cgatgtggta acccgacatg cgtagcgctg cccccacgct
     1201 tcctccgccc gcgacgcccg ctcgccaggc ggctgcgcag gctcaccagc ctgtcagaca
     1261 gcagtatctc gagctccgtg cggccgtcca ccgcaagctg ctcggacggt tgaacctcga
     1321 ggcgctcgcg cacgtggagc gcgcgaaggc cgaaggcgag atccgctccg tgctggccca
     1381 gctcctgtcc gaggaatcga cgccgatcag cctggcggag cgcgaaacgc tcttcggcga
     1441 ggtcctcgac gacgtgttcg ggctgggacc gctcgagccg ctgctgcgcg atccgagcat
     1501 cagcgacgtg ctcgtcaaca cgtacaagaa cgtcttcgtc gagcgaaagg gcgtcctcga
     1561 gcgcgtcacc gcgaccttcc aggacgacag ccatctgctg cgcgtcatcg atcggatcgt
     1621 gagccgcgtc ggccgccgcg tcgacgacag ctcgccgatg gtggacgccc gcctcgcgga
     1681 cggctcgcgt gtgaacgcca tcatcccgcc gctcgcggtc gacggtccgc tgctgtcgat
     1741 ccggcgattt ccggccgaac ggctgaaggc cgaggatctg gtgaacctgg gcgcgctgac
     1801 caagccgatg ctcgagtttc tcgcgcactg cgtgcgcgcg cacctgaact gcatcatcag
     1861 cgggggcacc ggcgccggca agacgacgct tctcaacatt ctgtccagct acatctcggc
     1921 tcgggaacgt gtcgtgacca tcgaggacgc cgcggagctg cagctccacc aggagcacgt
     1981 cgtgcggctc gagacgcgtc cgccgaacgt cgaaggcaag ggcgcgatcc gccagcggca
     2041 gctcgtcgtc aacgcgctgc gcatgcgccc cgaccgaatc gtggtcggcg aggtccgcag
     2101 cgaagaagcg ctcgacatgc ttcaggccat gaacaccggc cacgacggat cgctgacaac
     2161 ggtgcacgcc aacacgccgc gagacgccct gtcgcgcatc gagacgatga tcgcgatggg
     2221 tgcgacgaac ctgcccgaca aggcgatgcg gcagcagatc gcgtcggcca tccagctcgt
     2281 ggtgcagcag acccgcctgg ctgacggcac gcgcaagatc accagcatct ccgagatcac
     2341 cggcatggaa ggcgacgtga tcacgatgca ggagatcttc gtgttcgaga aagccggcgt
     2401 cagcaaggaa ggcaaggtca tcgggcgctt ccgcgcgacg ggtgtgcggc cgaagtgctc
     2461 cgagcggctc aaatcagcgg ggatcctgct gcccaccgag atgttcgaag gcgtcacgga
     2521 ggtgcggtaa tgcttctcgc cgccatcgtc ttcctggtcg tcacggcgat cgtcgccgcc
     2581 gtggccttcg gcgtcctgcg cctgccggga tcgctggccg cccgacggct ggagaagcgg
     2641 ctcagtgagg tctcgaaccg aaccgaggac gacgacaaac cggcgtcgtt catccgcagc
     2701 cacgccgcag gcccgctgcc ggcgctggat cgcgcattcg gcaggacggg cgcgctcgcc
     2761 atgctgatcg agcagtccgg cacgaagacg agcccgagca gcgtcgtcct tgccagcgtc
     2821 gccctggccg tcgcggcggc gattgccagc ctgttgttca tccgccagcc ctttgcctgg
     2881 gcgattgccg ccctggttgg cgcggcggtg ccgacgttct ggctgaagta caagcgctcc
     2941 gcgcggctga agcgcttcga agagcagttc cccgaggcgc tcgacctgct gtcgcgcgcg
     3001 attcgcgcgg ggcacgcgtt ccagacggcg atgggcatgt gtgccgacga gcttcccgct
     3061 cccgtgggcg tggagttcaa gaagagcttc gaccagcaga acttcgggct tccgttgaag
     3121 gaggcgctca acgagctctc cgagcgcgtg ccgattctcg acgtccggtt cttcgtgacg
     3181 gcggtgacga ttcagcgtga gaccggcggc aacctcgccg agattctcga caacctcgcg
     3241 cacgtggtcc gcgagcgctt caagattctg cggcaggtca gagtgcacac ggcgcacggc
     3301 cggttcacgg gctacgtgct cctgtcgctg ccggccgcgc tggcggtcgc gttgtcgtac
     3361 atcaaccctg gccacatgga cctgctgttc aaggagcaca tggggcagat gatgatcctg
     3421 acggcgatcg tcatgcagac gatcgggtac gtctggatcc gcaaagtgat caagatcgag
     3481 gtgtaggtgt gacgttcctg ctgcccgtcc ttgcgtttgc gttcgtcgcg ctgctcatcg
     3541 ccggcgccgc catggccctc tcgccgagcg ggagcatgct catcgagcag cgcctcggcg
     3601 agctgcgcgg cacgccggtc gataagtcgg tctcctacga gagagtcgcc aagacgctga
     3661 agcacatcgg caagtacgcg ccgcagtcgc cgtccgagct cggcaagctc cagaaacggc
     3721 tcgtgtgcgc cggctaccgc ggcaaggaag cgctgcccgt cttcatcgga atccggctgg
     3781 gatcagcgct gctcgcgttc ggcgtggcct cgtccccgct cgtcgtccgt ccgaacatct
     3841 tcatggcgct cagcgtggcc gccgtcgcgt acctccttcc gagcatggtg ctcggacggc
     3901 tcgcaaagaa gcgccagcac cgcatccgcc tgtcgctcgc cgacacgctg gatctgctgg
     3961 tcgtcagcgt ggaggccggg ctcggattgg atcaggcgct gcagcgcgtc ggcgaagagc
     4021 tcgccttcgc gcatcccgac ctgtccgacg aattgaggct catcaacctc gagctgcgcg
     4081 ccggcaagcc ccgatcggaa gcgcttcgca acctggccga ccgcaccggc gtggacgatt
     4141 tgtcgtcgct tgccgccatg ttgatccaga ccgacaagtt cgggaccagc gtggcgcagt
     4201 cgctgcgagt ccactccgac acgctgcgca cgaagcgccg gcagcgcgct gaagaagccg
     4261 cggccaagac cggcgtgaag atggtgttcc cgctcgtgtt ctgcatcttc ccggcgatct
     4321 gggtcgtaac catcggaccg gccgcgatca agttcgtgca ggtcctcttc ccgctggcac
     4381 aacagcaatg acgaccgtcc tggacgacgt ccgcctcgct ccgcctccag ccgttccttc
     4441 gacgctggaa gacacggggc tcggaaccga tcagatcgag cagttgctca tcaagacgct
     4501 gtacggcggt gaggcaacag gtctcgggct cgccgatcgc cttcggctga cctacacgat
     4561 tttcgagcgt gtcgtcgaac ggatccgcgc cgagcgcctg atcgaagtac ggggcacggt
     4621 gggatcgtcg gccagcccga actaccggta cgcgctcacc gatctcggcc gcgaccgggc
     4681 gcggcagtac ctggacatca accagtacac cggcgcggcg ccggttcccc tggcgaccta
     4741 cgtggcgcag atgcgtgcgc tggcggcctc gcgcggctac atcgagcgcg agcggcttcg
     4801 caacgggttc tcccatctga tcgtgggcga cgacatcctc gagcaactcg ggccggcggc
     4861 caacgccaac aaggcgatct tcctgtacgg ccccccgggc aacggcaaga cggtgcttgc
     4921 cgagggcctg ggcagggcca tcggcggcga catgtacatg ccgtacgcca tcgaggtcga
     4981 ggg
//
LOCUS       DBNGSH010000096         1678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1874604, whole genome shotgun sequence.
ACCESSION   DBNGSH010000096 DBNGSH010000000
VERSION     DBNGSH010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1874604"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /gene="alaS"
                     /locus_tag="AC16CY_02170"
     CDS             complement(<1..1646)
                     /gene="alaS"
                     /locus_tag="AC16CY_02170"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR3352610.1"
                     /translation="MGLERVTAVLQGRLSNYDTDLFTPLLAAIGSRAGVHHGGTGGAA
                     DVSMRVVADHMRAMTFLIADGVAPSNEWRGYVLRKVMRRAMRHGKRLGIDQPFLHTLV
                     DTIVEGFGDAYPELRSGRDLVVQVVRNEEERFDAVLAGGLPRLEEVLTQAAAGNGIVA
                     GDAAFKLYDTYGLPRDFIEDMIEDRKLRFDEPGFERAMGAQREKARAGSAFKGQGVTE
                     VAWTASDATRQALVSAGDRGFRGYETTSSRTPILALFDADRNEVTSLPSGKDGYVALA
                     ETPFYVEAGGQVSDVGTIAGPRGEAEVTGVVRAGSWPRLHRVRITSGELATRELVSAD
                     VTASVRDATRRNHTATHLLHAALRQVLGTHVKQAGSLVAPDRLRFDFVHVSALTDEQR
                     LEIERLVNEHVLRNTPVQTEVKDTQAAIAAGAMALFGEKYGDQVRVVSIPGFSLELCG
                     GTHVRATGDIGLFAIVAESGVAAGVRRIEAITGLDSVAAFQRQRDELDALSAALNARA
                     GELSARVAALQDDNKRLVRDLQEAKMKAALTTSSGGGAQD"
BASE COUNT          250 a          596 c          555 g          277 t
ORIGIN      
        1 tcgtcctgcg cgccgcctcc tgaagaggtt gtgagcgcgg ccttcatctt cgcctcttga
       61 aggtcgcgaa cgagccgctt gttgtcgtcc tgaagcgcgg ccacccgcgc gctcagctct
      121 cccgctcggg cgttgagcgc ggcggagagc gcgtcgagct cgtcgcgctg acgctgaaac
      181 gccgccaccg agtcgagccc ggttatcgcc tcgatccggc gcacgccggc tgcgacgccg
      241 ctctccgcga cgatggcaaa gagcccgatg tcaccggtgg cacgcacgtg cgtgccaccg
      301 cacagttcca ggctgaatcc cggaatcgag acgacccgta cctgatcgcc gtacttctcg
      361 ccgaacagcg ccattgcgcc cgcagcgatg gctgcctggg tatccttgac ctcggtctga
      421 accggcgtgt tccgcagcac gtgttcgttg acgagacgct cgatctcgag acgctgctcg
      481 tcggtcaggg cgctgacgtg cacgaagtcg aaccgcaggc gatcgggcgc cacgagcgag
      541 ccggcctgct tgacatgggt gccgaggact tgccgcaggg ccgcatgcag caggtgagtc
      601 gcggtgtggt tccggcgtgt ggcgtcgcgc acgctggctg tgacgtccgc tgacaccagc
      661 tcgcgggtcg ccagctcgcc gctcgtgatt ctgacgcggt gaaggcgcgg ccacgagccg
      721 gccctgacca cgcccgtgac ctccgcctcg cctcgcggac cggcgatcgt ccccacgtcg
      781 gatacctggc cgcccgcctc gacgtagaac ggcgtctccg cgagcgcgac gtaaccgtcc
      841 ttcccggacg gcagcgaggt cacttcgttt cgatcggcat cgaagagggc gagaatcggt
      901 gttctggaag acgtcgtctc gtagccccgg aatccacggt cgccggccga cacgagggcc
      961 tgacgggtcg cgtcgctcgc agtccacgcg acctccgtca ctccctggcc tttgaacgcg
     1021 ctcccggcgc gcgccttttc gcgctgcgcc cccattgcgc gctcgaatcc gggctcgtcg
     1081 aatcgcagct tccggtcttc gatcatgtcc tcgatgaagt cacgcggcag cccgtaggtg
     1141 tcgtacagct tgaacgcggc gtcgccggcg acgataccgt tgcccgccgc ggcctgcgtg
     1201 agcacctctt cgaggcgagg cagcccgccc gcgagaaccg catcgaagcg ctcctcctcg
     1261 ttgcggacca cctgcacgac gaggtcgcgc ccggacctca actccgggta ggcatcgccg
     1321 aacccttcga cgatggtgtc cactaacgtg tggagaaacg gctggtcgat gccgagccgc
     1381 ttcccgtggc gcatggcgcg ccgcatgacc ttccgcagga cgtacccgcg ccactcgttg
     1441 gagggagcga cgccgtcggc gatgaggaac gtcatcgcgc gcatgtgatc cgccaccacg
     1501 cgcatcgaga cgtcggccgc cccccccgtg ccgccgtgat ggacgccggc gcggctgccg
     1561 atcgcggcaa gcaggggcgt gaaaaggtcg gtgtcgtagt tcgagaggcg gccctggaga
     1621 acggccgtca cccgttcgag tcccatcccg gtatcgatgg acggcgccgg cagagggt
//
LOCUS       DBNGSH010000097         3293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3232663, whole genome shotgun sequence.
ACCESSION   DBNGSH010000097 DBNGSH010000000
VERSION     DBNGSH010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3232663"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1870
                     /locus_tag="AC16CY_02175"
     CDS             44..1870
                     /locus_tag="AC16CY_02175"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HIR3352611.1"
                     /translation="MACVAAIFNTDGAPVDRTALGSLMDVEPYRRPGGQHLTIDGCVG
                     LGCAPLAAESIGDQARQPRGLENRLWAVLDGRLDDRGSLIAALQPSSARSRVEPRSDV
                     DMILSAYDCWGVECVSHLTGDFAFCLWDAQRRRLFCARDRFGVKPFYYAHVGRTLLVC
                     STLRTLRRHPLVSDRLRDEAIGDFLLFGLCVEPSQTSFADLQRLPPAHRLTCSPEGPP
                     RVDRYWRLEPADLDRESDPRHRVERFSRLMREAVADRIRSDRTAVLMSGGLDSSSVAS
                     VAAEIARPKTDRLHAYTLVYDAQARDEERRYSSIVARSLGIGITHLPVDDYEPFARWD
                     RDALPPEPSLEGLSAVMSDVLDLASAHGHVVLTGEGGDPLLLPSTIIEQVGWVPFSSL
                     AGDVWRACRAGSLPPVGLRSTLRGWFRRSRGVPTWLADGLLRAFDPHERWREVGAART
                     AARGARSRALNDLVDPWWPSTFESLDPGATGRPVQVRYPFFDVRLVTYALTLPTPWCV
                     NKRILRASMRDRLPGAVLARPKTPLQADPVGPRGRWSLERATRLLASTPDITRYVNVD
                     RFRSTVRHDSLLTDRSPGTWAAISLANWIRCSAGGPAPTELN"
     gene            1867..2847
                     /locus_tag="AC16CY_02180"
     CDS             1867..2847
                     /locus_tag="AC16CY_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352612.1"
                     /translation="MSDHHYEAFGLSIACNAPIPGLPSAADTDSPDLYVLMGDDSLDE
                     GSIPESVSYLSSSRDDSGTPLLTVWTRPGGGHRLRYCDGAEYTVDHDASRVHVRWEAP
                     LSSADAAIYLLGPVLGFVMRLRGIVPLHASAVAVGGRAIVFAGDAGMGKSTTAVAFAA
                     LGYPVLSDDVVPIVLTENGVLAYPSHPRLTIWPESVEGLFGSAAALPALTATYDKRYL
                     DLVGRFRFQSTPLPIEVIYVLSGRAPSPASFGVRTLKPQAALLTLVGHTYGNYLLDGP
                     MRASEFDCVSQVAERIPVRELTLGAGFSELPRVCEELVTKGPREAAATTP"
     gene            2844..2993
                     /locus_tag="AC16CY_02185"
     CDS             2844..2993
                     /locus_tag="AC16CY_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352613.1"
                     /translation="MSENTRTGPKKPYAAPRLVTYGDIAQLTQNSPSAKQKADGGGKL
                     KTKTI"
BASE COUNT          497 a         1097 c         1105 g          594 t
ORIGIN      
        1 ggtggacgcc tcgcggagaa tgtcgtagtc tgccgcacca gtcgtggcct gtgtagcggc
       61 aatcttcaac accgacggcg cgcccgtgga ccggaccgca ctcggttcgc tgatggacgt
      121 cgagccctat cgacggcctg gcggccagca tctcacgatc gatggctgcg tcggccttgg
      181 ctgcgcgccg ctcgctgcgg agtcgattgg cgaccaagcg cggcagcccc gtggcctgga
      241 gaatcgactc tgggccgttc ttgacgggcg gctcgacgat cgaggctctc tcatcgcggc
      301 gcttcagccc tcatcggcgc gatcccgcgt cgagccccgc tccgacgtcg acatgatcct
      361 gagcgcctac gattgctggg gcgtggagtg cgtctcgcac ctgactgggg attttgcgtt
      421 ctgcctctgg gacgcgcagc gtcgtcgcct gttctgcgcc cgcgaccgtt tcggggtcaa
      481 gccgttctac tacgcacacg tcggcaggac gctcctcgtc tgcagcacgc tgcgcacgct
      541 gaggcgtcat ccccttgtga gcgatcgact gcgtgacgaa gcgatcggcg actttctgct
      601 cttcggtctg tgtgtggagc cgtcgcagac gtctttcgcg gacctgcagc gccttcctcc
      661 ggctcatcga ctgacgtgct caccggaggg accacctcga gtcgatcgct actggcggct
      721 cgagccggcc gatctcgacc gcgagtccga cccacgccac cgcgtggagc gcttttctcg
      781 gctgatgcgc gaagccgtgg cggaccgcat ccggtccgac aggacggcgg tgctgatgag
      841 cggcgggctc gactcctcca gcgtggcctc cgtcgcggcg gagatcgccc gtccgaagac
      901 cgacaggctt cacgcctaca cgctcgtcta cgacgcgcag gcacgggacg aagagcgccg
      961 gtactcgtcg atcgtcgcgc gctcgctggg gatcggcatc acccacctgc ctgtggacga
     1021 ttacgagccg ttcgcgagat gggaccggga cgctctgcct ccggagccgt cgctcgaggg
     1081 gctgagcgcc gtcatgtccg atgtgctcga cctggcatcc gctcacgggc acgtcgtcct
     1141 caccggggag ggtggggatc cgctcctcct gccgtcgacg atcatcgagc aggttggctg
     1201 ggttccgttc tcgagtctgg ccggagacgt atggcgcgcg tgtcgcgcag gctcgctgcc
     1261 gccagtcgga ctgcgctcga cgctcagggg ctggttccgc aggtcccgcg gcgtgcccac
     1321 gtggctggca gatgggctgc ttcgagcctt cgatccgcac gaacgctggc gtgaggtagg
     1381 ggccgcccgc accgcggcgc gcggcgcgcg gagccgcgcg ttgaacgacc tggtggatcc
     1441 gtggtggccg tcaaccttcg agagcctcga tccgggcgca acgggacggc ccgtacaggt
     1501 gaggtaccct ttcttcgacg tgcgcctcgt gacgtatgcc ctgacgcttc cgactccctg
     1561 gtgcgtcaac aagcggatcc ttcgtgcgtc catgcgcgat cgcttgccgg gggcagtcct
     1621 cgcgcggccg aagacgcccc ttcaggccga ccccgtcggt ccgcgcggcc gctggtctct
     1681 ggagcgagcc acgcgtctgc tcgcgagcac tcctgacatc acccgctacg tgaacgtcga
     1741 cagatttcga tcgacggttc gccacgactc cctcctgaca gaccgcagcc cgggcacgtg
     1801 ggcggcgatc agcctggcga actggatacg atgcagcgcc ggtgggcccg cgccgacgga
     1861 actcaattga gcgaccatca ttacgaagcg ttcggactgt ccatcgcgtg caacgcgccc
     1921 attcctgggt tgccatcggc tgcggacaca gactctcccg acctgtatgt cttgatgggc
     1981 gacgacagtc tggacgaggg ctcgattccg gagtcggtta gctatctcag ttcctcgcgc
     2041 gacgattccg gcaccccgct gttgacggtc tggacgcggc ccggcggagg acaccgcctc
     2101 cgctattgcg atggcgctga gtacaccgtc gatcacgatg cgtcgcgcgt ccacgtgcgc
     2161 tgggaggccc cgctgtcgag cgctgacgcc gccatttatc tgctcggccc cgtcctcgga
     2221 ttcgtcatgc ggcttcgcgg aatcgttccg ctgcacgcca gcgccgtcgc ggtcggcggc
     2281 cgggccattg tgttcgcagg cgacgcggga atgggaaagt cgacgacggc cgtcgcgttt
     2341 gccgccctcg gctatccagt gttgtccgac gacgtcgtac ccatcgtgct caccgagaat
     2401 ggtgtacttg cctacccgag tcatccgcgg ctcacgatct ggccggagtc cgtcgagggg
     2461 ctgttcgggt ccgcagccgc actgccggcg ctgacggcga cctacgacaa gcgatacctg
     2521 gacctggtgg gccgatttcg gttccagtcc actccgcttc cgatcgaggt aatctacgtc
     2581 ctgagcggca gagccccctc gcccgcgtcg ttcggcgttc gcaccctgaa gccgcaggcg
     2641 gcattgttga cgctggtcgg gcacacgtat ggcaactacc tgctggatgg cccgatgcgc
     2701 gcgagcgagt tcgactgcgt gagccaggtc gccgagcgga ttccggttcg cgagttgacg
     2761 ctcggcgcgg gcttctcgga actcccgcgg gtatgcgagg agctggtgac gaagggaccg
     2821 agggaggcgg ccgcaacgac gccatgagcg agaacacacg gacaggacca aagaagccgt
     2881 acgcggcgcc tcgattggtc acctacggcg atatcgctca gctgacgcag aacagtccgt
     2941 cagcgaagca gaaagccgac ggagggggca agctgaagac gaagacgatc tagcgccagc
     3001 gcgctcttca agggacgacg cgatggtcca catcgggtta cacatctggc catcgggctc
     3061 tgtcgagggc gcgacactgg aacggaatgg tccggttcgc gaaggttcct cgtggcgcga
     3121 ggattgcaga ttcgcgcggc gtggcgttta catcgtttac aacgtcaacc gccgagcggg
     3181 ctcccgcgag agtgcgcacg atcctcggtt ggactcccgg gctgcgaact cttgtctcga
     3241 gcggggtgct cccgtgcgga tgcctggccg gcacctatga gacgtggacg aag
//
LOCUS       DBNGSH010000098         4739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2383965, whole genome shotgun sequence.
ACCESSION   DBNGSH010000098 DBNGSH010000000
VERSION     DBNGSH010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2383965"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2121
                     /locus_tag="AC16CY_02190"
     CDS             <1..2121
                     /locus_tag="AC16CY_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3352614.1"
                     /translation="MASRLGGEGMAVESRRLRVVSAVAVEGRDEAALLALAGSVAGGL
                     DPQVAAAIRESARERDIQVREADRFRDIAHVGVSADVAGKTVVLGDAARLAGLGVSVE
                     GFGDLPERLRRRGEHVLFLAVNGRAAGFFGLAPSSHGNTRQEVMEDQMNASLPAADRF
                     PTLTEGLPAALATDVVALADGDRFDLRIAPVSKRLGGTEVRMLAYNGSVPGPTLRVQQ
                     GSQLVVNVVNDGDLDATVHWHGLRLENRFDGTTETQPPIPVGGTFTYRLTFPDPGIYW
                     YHPHIREDYGQEMGLYGTVVVVPSEPDYWPAVNRELSLTLDDILLEHGAVAPFSRTHP
                     TYTAMGRFGNVLLVNGEPEPSLRAKQGEVVRLYLTNTANTRVFNVGVAGARMKLVGGD
                     SGRCERETFVDGVLIAPSERAVADVLFGQPGQVRLEHRTPDRTYELGTITVEAEPAEP
                     SLASTFGILRANSDLIAERGRVAGYLDAAPDEILALVAEMDMGERQAPPGAAVEYVCP
                     MHPEITSKQPGRCPKCGMKLVPADLVGAASHGEHEHADTGLDRHDDHQHDHEARNDNW
                     PAGIEWEDDMVDVNRMTNPANMRWKLIDRQTGAEGRSIDWRFHAGDQVKIRLVNEMDS
                     DHPMPHPFHVHGAGRFLILSRDGVVEPNLVWKDTVLVPTGQTVDILLEVTHPGVWMAH
                     CHIAEHHESGMMLRFQVDPARNES"
     gene            2131..3624
                     /locus_tag="AC16CY_02195"
     CDS             2131..3624
                     /locus_tag="AC16CY_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HIR3352615.1"
                     /translation="MRTTRSPFILLLGSSIALLVGFVVAQDTRGDSTRAPAAPARYVT
                     VPEGAGDRLAGAVRIRTISSEDPDAFDAEAFDAIHAYLRAAFPRVHRQLRRESVATHS
                     LLYSWPGSDSSLKPLLLAGHLDVVPVEPGSDGKWQHDPFSGSIADGFVWGRGAIDNKA
                     GVLGALEAVEMLLAEDFRPTRTVYLAFGHDEEVGGARGAREIAALLKARGVELEMVLD
                     EGGVIGDGVLPGLSQPVALVGIAEKGFVSLELSVRTAGGHSSLPPPQSAVGILSAAIA
                     RLEANPMPVRLEGPTRQLFDRIRPRLPLLQRTMLANLWLTRPLLLRRLEKNSGTNAMV
                     RTTAAPTIFQAGTKDNVLPADAMAVVNFRILPGDTVATVVEHVTRVVGDDRVSVKTAG
                     RFAAEPSEVSSIDSEAFRTLERTIRSVAADVIVAPYLVVVVTDARYYGGLSRNVFRFL
                     PLRLTSRELERMHGIDERIGIREYEAAIRTYRQLLIDAAGGRVDATS"
     gene            complement(3650..4030)
                     /locus_tag="AC16CY_02200"
     CDS             complement(3650..4030)
                     /locus_tag="AC16CY_02200"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3352616.1"
                     /translation="MSAANGLSPDEKTLYVTNGGVVIAFDVGPDGALTRQREFGKLRG
                     GQGGDGSAVDAQGRLYVSTGASVDVFTPAGEFLGSIVGPQGLHGTAFGGRDEDALRHR
                     LLRHVGHGQRAKPGDRDSDDHGGL"
     gene            complement(4009..>4739)
                     /locus_tag="AC16CY_02205"
     CDS             complement(4009..>4739)
                     /locus_tag="AC16CY_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3352617.1"
                     /translation="AKGIPLVPVMKSATALAVLLACAAAIASAQSPSSAFARGNSLQS
                     WQNPGLKSVLAKCKTPPAPFAIPGAGTANAKPAPPPEPPAPPASTAIPGVIAAGQTWK
                     VVWSWEGNNADGIIAGDEGTMLFANNDASNVMKLDPATGLASIIHTDTNTRGALSRSR
                     NGALFLASRGLNAGILQLEPQRKSFATSFRGEPLDCAGGVVNDLVADGRGGVYVSITG
                     GGLFDANPKGVVSQYGRACQRPTA"
BASE COUNT          774 a         1565 c         1593 g          807 t
ORIGIN      
        1 atggcgtcgc gcctcggggg agaagggatg gcggtcgaaa gccggagact gcgagtcgtc
       61 tcggccgtcg ccgtggaggg tcgtgacgaa gcggctctcc tcgcgctggc cggcagcgtg
      121 gctggcggtc tcgaccctca ggtggccgcc gcgatccggg aatccgctcg cgagcgcgat
      181 atccaggttc gcgaggccga tcggtttcgc gacatcgcgc acgtcggggt ctcggcggat
      241 gtcgccggca agaccgtcgt cctcggggac gccgcgcgcc ttgccggcct cggcgtgtcg
      301 gtcgaaggtt tcggcgacct gcccgagcgc ctgcgccggc gcggcgaaca cgtgctcttc
      361 ctcgctgtca acggccgcgc ggccgggttc ttcgggctgg ccccgtcgag tcacggaaac
      421 acccgtcaag aagtcatgga ggaccagatg aacgcttcac tgccggccgc cgatcgcttt
      481 ccaaccttga ccgaaggtct tcccgcggcg ctcgccaccg atgtcgtcgc gttggccgac
      541 ggcgatcggt tcgaccttcg aattgctccg gtgagcaaac gactcggtgg caccgaggtg
      601 cgcatgctcg cctacaacgg atccgtgccg gggcccacgc ttcgcgtgca gcagggatcg
      661 caactcgtcg tcaacgtcgt gaacgacggc gacctcgatg cgaccgtgca ctggcatggc
      721 ctgcgtctgg agaaccgttt tgacggcacc accgaaacgc aacccccgat tcctgtcggc
      781 ggcacgttca cgtaccggct gacattcccg gatcccggca tctactggta tcacccgcac
      841 atccgcgagg actacgggca ggagatgggt ctttacggca ccgtcgtcgt ggtcccgtca
      901 gagcccgact actggcccgc ggtgaatcgt gagctgagcc tcactctcga cgacattctg
      961 ctggagcacg gagcggtcgc cccgttcagc cgcacgcatc ccacgtatac cgccatgggc
     1021 cggttcggga acgtcttgct ggtcaacggc gagccggaac cgtcacttcg cgcgaagcag
     1081 ggtgaggtcg ttcgcctcta cctgacgaac acggccaaca cgcgcgtctt caacgtgggt
     1141 gtcgccggcg cgcggatgaa gcttgtcggc ggtgacagcg ggcgctgcga gcgggagacg
     1201 ttcgtggacg gcgtgctgat tgccccgtct gaacgggctg tggcggatgt gctgttcggg
     1261 cagccgggtc aagtgaggct cgagcaccgg acacctgatc ggacgtacga gctgggcacg
     1321 atcacggtcg aagcggaacc cgccgagccg tcacttgcgt cgacattcgg aatcctgcgg
     1381 gcgaactccg acctgatagc cgagcgcggc cgggtggccg gctacctcga tgccgcaccc
     1441 gacgagatac tggcgctcgt tgccgagatg gacatgggcg agcggcaagc gccgcccggc
     1501 gcagccgtcg agtatgtctg cccgatgcat cctgagatca cgagcaagca accgggacgc
     1561 tgcccgaagt gcggcatgaa gctcgtgccg gccgacctgg ttggagccgc gtcgcacggc
     1621 gaacacgaac atgccgatac cgggctcgat cggcatgacg accatcagca cgaccacgag
     1681 gcccgaaacg acaactggcc agccggtatc gagtgggaag acgacatggt ggacgtgaac
     1741 cgaatgacca atcccgccaa catgcgctgg aagctgatcg accgccagac gggtgcggag
     1801 ggtcggtcga tcgactggcg attccacgcg ggcgaccagg tgaagatccg gctggtcaac
     1861 gagatggact cggatcatcc gatgccccat ccgtttcacg tccacggcgc cggacgtttt
     1921 ctgatcctgt cgcgggatgg cgtggtggaa ccgaatctcg tctggaagga caccgtgctc
     1981 gtcccgaccg gacaaacggt cgacattctg ctcgaagtca cgcatccagg tgtgtggatg
     2041 gcgcactgcc acattgccga gcaccacgag agcggaatga tgctccgctt ccaggtggat
     2101 cccgcgcgca atgagtcgta ggcaccgccg atgcgcacca ccagatcgcc gttcatcttg
     2161 ctgctcggct cgtcaatcgc cctgcttgtc ggattcgtcg ttgcccagga cacgcgcggc
     2221 gattcgacac gggcgccagc cgccccggcg cgctacgtca cggtgcccga gggtgccggg
     2281 gaccggctcg ccggtgcggt ccgcatccgg acgatctcgt ccgaggatcc tgacgcgttc
     2341 gatgcggagg ccttcgacgc catacacgcg tatcttcgag cggcgtttcc gcgagtccac
     2401 cgacagctcc ggcgggagag cgttgcgact catagcctct tgtactcgtg gccaggcagc
     2461 gactcatcgc tcaagccgct gctgctggcc ggtcacctcg acgtggtccc ggtcgagccg
     2521 ggctccgacg ggaagtggca gcacgaccct ttcagtggca gcatcgccga cggctttgtc
     2581 tgggggcgcg gcgccatcga caacaaggcc ggcgtcctcg gcgccctcga agcggtggag
     2641 atgcttcttg ctgaagactt tcgtccgacc cggacggtgt atctggcctt cgggcacgat
     2701 gaggaggtcg gcggagcgcg cggcgctcgc gaaatcgccg cgctgctgaa ggctcgtggc
     2761 gtcgagctgg agatggtgct ggatgaaggc ggcgtgatcg gtgacggcgt cctgcccggc
     2821 ctctcccagc cagtcgcgct ggtgggaatt gccgagaagg gcttcgtctc tctcgagctg
     2881 agcgtccgca cagccggagg gcattcttca ctgcctccac cacagagcgc ggtcggcatc
     2941 ctgagcgctg cgattgccag gctggaggcg aacccgatgc cggttcggct ggaagggccg
     3001 acgcggcaac tgttcgaccg gatcaggcct cggttgccgt tgctgcagcg aacgatgctg
     3061 gcaaacctct ggctgactcg tccacttctg ttgcggcgcc tcgagaagaa ctccggcacc
     3121 aacgctatgg tccggacgac cgcggcaccg acgatcttcc aggcgggaac gaaagacaac
     3181 gtgcttccag ctgacgccat ggcggtcgtc aacttccgca tcctgcccgg cgacacggtc
     3241 gcgacagtcg tggagcatgt cacccgggtg gtcggcgatg accgtgtcag tgtcaagacg
     3301 gccggacgtt ttgccgccga accgtcggag gtgtcgagca tcgactccga ggccttcagg
     3361 acgctcgagc ggacgattcg aagcgtggct gcagacgtga tcgtcgcgcc ctatctcgta
     3421 gtggtcgtga ccgatgcgcg ctattacggc ggcctcagcc gcaacgtgtt caggtttctt
     3481 ccgctgcgcc tcacgtcacg ggagctcgag cgcatgcacg gaatcgacga gcggatcggc
     3541 atccgcgagt acgaggctgc tatccggacc tatcgtcaac ttctgatcga tgccgccggg
     3601 ggacgagtgg atgccacttc gtgaatcgcg tgatcacttc gcccgacctt tataaccctc
     3661 cgtgatcatc ggaatcgcga tcacccggtt tcgcgcgctg gccgtgcccc acgtgccgta
     3721 gaagacgatg gcgaagagcg tcttcgtcgc ggccgccgaa ggccgtgccg tgcagcccct
     3781 gcggccccac gatgctgccg aggaactcgc cggccggggt gaagacgtcc accgatgccc
     3841 cggtggacac gtacagacgc ccctgcgcat cgacggccga cccgtcgccg ccctggccgc
     3901 cgcgcagctt tccgaactcc cgctgcctcg tcagcgcgcc gtcgggcccg acgtcgaagg
     3961 cgatgaccac cccgccgttg gtgacgtaca gcgtcttctc gtccgggctc aggccgttgg
     4021 ccgctgacat gccctcccgt actgcgagac gacgcccttc ggattggcgt cgaacagccc
     4081 gccccccgta atcgagacat acacgccgcc gcggccgtcg gcgacgaggt cgttcaccac
     4141 gccgccggcg cagtcgagcg gctcgccgcg aaaggaggtc gcaaacgatt tgcgctgcgg
     4201 ctcgagctgc aggatgccgg cattcagccc cctggacgca agaaacagcg cgccgttcct
     4261 gctgcgcgag agggcgccgc gggtgttggt atccgtgtgg atgatcgacg cgaggcccgt
     4321 cgccgggtcg agcttcatca cgttgctcgc gtcgttgttg gcgaacagca tcgtgccctc
     4381 gtcaccggcg atgatgccgt cggcgttgtt gccctcccac gaccacacga ccttccacgt
     4441 ctgccccgcg gcgatcacgc cgggaatggc tgttgacgcg ggcggagccg gcggctcggg
     4501 cggcggcgcc ggcttggcgt ttgccgtgcc cgcgcccgga atcgcgaagg gcgccggagg
     4561 ggtcttgcac ttggcgagga cgctcttcag tccgggattc tgccaggact gcaagctgtt
     4621 gccgcgggca aaagcagaag acggactctg agccgaggcg atggctgccg cgcaggcgag
     4681 gagcacggcg agcgctgtcg ctgatttcat gaccggcact agtggtatgc ccttcgcgg
//
LOCUS       DBNGSH010000099         1695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2384072, whole genome shotgun sequence.
ACCESSION   DBNGSH010000099 DBNGSH010000000
VERSION     DBNGSH010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2384072"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC16CY_02210"
     CDS             complement(<1..586)
                     /locus_tag="AC16CY_02210"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3352618.1"
                     /translation="MFFRGQTIGKYRIESSLGSGGFGVVYLAEDTWIKKKVALKVPHK
                     QQLDFSELLKEPRLLASLSHPNIVAVLTAERQEKVFFIVMEYVQGETLEHLIMREGAL
                     DLSLAQDFSCQICNAVDHAHRAGILHRDLRPGNMLISDAGMLKVTDFGTSRFLEIAAH
                     GTTIIGSPAYMAPEQFHGKAVFASDIYSVGVTMYQ"
     gene            671..1420
                     /locus_tag="AC16CY_02215"
     CDS             671..1420
                     /locus_tag="AC16CY_02215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR3352619.1"
                     /translation="MPARFFAPDANGPGDFVTLPEDEGEHLTRVLRLGTSDVVRVFNG
                     RGDEFESVVETADRGRVGVRVGLPCDPAPEPGLSIALAQAVLKRDKMDDVVRDAVMMG
                     VTAIHPILTSRTEVPSTAFENGRRRDRWARVAVSSAKQCGRAVVPPVSEPITLEAFGR
                     KVLESWMTGPALMLVEPGASRKAVPLGELDVERPRTATVIVGPEGGWTPEEIQLGSSV
                     CRLVTLGERTLRADSMALVALAATFTKWGEF"
BASE COUNT          297 a          514 c          587 g          297 t
ORIGIN      
        1 tctggtacat cgtgacgccc acggaataga tgtcgctggc gaagaccgcc ttgccgtgaa
       61 actgctccgg ggccatgtac gccgggctgc cgatgatcgt cgtgccgtgc gcggcgatct
      121 cgaggaaccg cgacgttccg aagtcggtga ccttcagcat gccggcgtcg gagatgagca
      181 tgttgcccgg gcgcaggtcg cggtgaagga tccccgcgcg atgggcgtga tccaccgcgt
      241 tgcagatctg gcagctgaag tcctgcgcga gagagagatc gagcgccccc tcgcgcatga
      301 tcagatgctc cagcgtctcg ccctgcacgt actccatgac gatgaagaac accttctcct
      361 gcctctcggc ggtgaggacg gcgacgatgt tcggatggct cagggaggcg aggagccgcg
      421 gctccttgag cagctcgctg aagtcgagct gctgcttgtg cggaaccttc agcgcgacct
      481 tcttcttgat ccaggtgtcc tcggcgaggt agacgacgcc aaagccacca ctgccaaggg
      541 atgattcgat gcggtatttg ccgatggtct ggccgcgaaa gaacatcgag gggtggaggg
      601 agtatagcag ccgccgggct actcgcggcg gcgtgccatc cgtgatcagc gccggttcta
      661 taatccgtgc atgcctgccc gcttcttcgc tcctgacgcg aacggcccgg gcgatttcgt
      721 gacactgccg gaggacgagg gagagcacct cacgcgcgtg ctgcgtctgg ggaccagcga
      781 cgtcgttcgc gtcttcaacg gacggggtga cgagttcgag tcggtcgtcg agaccgccga
      841 ccggggccgc gtcggcgtac gcgtgggcct gccgtgcgat ccggctccgg agccggggct
      901 gtcgatcgcg ctggcgcagg ccgtgctcaa gcgcgacaag atggacgacg tcgtgcgcga
      961 cgcggtgatg atgggcgtga cggcgataca ccccatcctg acgtcacgga ccgaggtgcc
     1021 atcaacggcc ttcgagaacg ggcggcggcg cgatcgatgg gcgcgcgtcg ccgtctcgtc
     1081 cgccaagcag tgcggcaggg ccgtcgtgcc gccggtgtcc gagccgatca cgctcgaggc
     1141 gttcggccgg aaggtgctcg aatcgtggat gacggggccc gcgttgatgt tggtggagcc
     1201 aggcgcgtca cgcaaggcgg tcccgctcgg cgagctcgac gtcgagcgtc ccaggaccgc
     1261 cacggtcatc gtcggccccg aaggtggctg gacgccggaa gagatccaac tgggctccag
     1321 cgtctgccgg ctcgtcacgc tgggcgagcg gacgctgcgc gcggactcga tggcgctcgt
     1381 ggcgctggcc gcgaccttca cgaagtgggg agaattctga acaggagacc cactgtcgcg
     1441 gactgaagaa cctgtcgttc acacttctcg cttctcactt ctcggttcgt gttcaatttc
     1501 aagttcgtgt tcggggttcg acgtttgggg ttcgtacccc ggggcaccgg atgggacggt
     1561 ccgcgtgaat cgcagccacc tacgccgtca ttgcgaatat ggctgactgt caggaactaa
     1621 gactcggcga acgtggaaca gcgaacctaa gaacacgaac cagaaactga acacgaaccg
     1681 agaagtgaga agcga
//
LOCUS       DBNGSH010000100         4929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2044861, whole genome shotgun sequence.
ACCESSION   DBNGSH010000100 DBNGSH010000000
VERSION     DBNGSH010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2044861"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="AC16CY_02220"
     CDS             <1..744
                     /locus_tag="AC16CY_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352620.1"
                     /translation="EFGADIVGICEIEPSDVYRGRTVTDKYAVAVGQRMRWREFQVVP
                     SRESAIECLRVYFTLGESVIQLAAHIRSLGYACKVEHPIGDSDLLHIPIGLKAGFGEL
                     GRHGSIIHPRLGPLFRMGSVITSMALEIDHPIDAGIARFCDSCRACRKFCPPQAIPDE
                     RSPEAGRDHLGYDRYVVDTGRCFPYFAKYSYCSICLPVCAYNHKEWARDFEGFQTKIF
                     PKVIMLEPPPPADLPAGGPCHDYPRINRS"
     gene            741..2042
                     /locus_tag="AC16CY_02225"
     CDS             741..2042
                     /locus_tag="AC16CY_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3352621.1"
                     /translation="MKAARLGAWSATAMVVSQVVGVGIFLTPATMMRTIGSAPAALAV
                     WAVMGALSAAGALCYAELSTRFPKAGGGYVFLREAFGPRCAFLYGWMSLLVVDPGLTA
                     ALGIGFGQYLLAAVGGPQDLVPFVAIGAIVLFGSLTLVGVNASSRVMQWTAAAKLAIV
                     ALLVLAALLQTSSLASRWTAAAPATLTPDALAASLVAAFFAFGGWWEFGRMSEEVASP
                     RRTMPRALVGGLALVTGMYALVSMAYTLAAPGGVQTTTEAFVAAVGSALFGEAAGRLL
                     AVMVVVAVAGSLAATLLGAPRVYLAMARDRLFPQRLARFDPDRGTVPGSTIVQVVLAS
                     ALALVGSFGDVLGYFVPCAVFFLGLSAASVLVLPRPEARADVFVAPFHPLPIVLFLVM
                     VSSVLVLFVLGQPRQTLLGALVVLLGVPVSWLVVPRRSSRG"
     gene            2126..2533
                     /locus_tag="AC16CY_02230"
     CDS             2126..2533
                     /locus_tag="AC16CY_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352622.1"
                     /translation="MSRRSVVALLMFLLPSASAAADLRITDSRGAEVLLTGASIDYSG
                     FVAEDKETQGVRVLQGEGRVTVKWTDVESLKVVRVDESVRPSRIEIEIVLKNGKKVPA
                     ALHRQGQMKLLGKSDLGDYSIELDKVRSIVPVH"
     gene            2642..3292
                     /locus_tag="AC16CY_02235"
     CDS             2642..3292
                     /locus_tag="AC16CY_02235"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3352623.1"
                     /translation="MFVGHLALALGTRYVEPRVPLGAAVAASYGLDLLWPVLLLTGAE
                     SVRVQAGDTAFTNLSFTSYPWSHSLLVVAGWSALTMILAQRVYGSARIGRFLGALVLS
                     HWVLDVVTHRPDLPLWPGGPVVGLGLWNSIAATIVVEGALLAAGLWLYLRASAARDQI
                     GRLALTALVAVTSLIWISQPWSPPPPSAAAVAWGALILWLLPPWAAWIDAHRASRT"
     gene            complement(3302..3967)
                     /locus_tag="AC16CY_02240"
     CDS             complement(3302..3967)
                     /locus_tag="AC16CY_02240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3352624.1"
                     /translation="MKAISVLGVLAFSIALAAQQPGVHPVSGRVYARTMSVEGAPWLE
                     RREREREEDPDLALRLLRIRRGSTVADVGAGSGYYTIRLARIVGPMGKVYASDIQPGM
                     IALIQQNVARAKIDNVVPVLGTIDDPKLPPASIDLAIMVDVYHEFSQPQRMLQRIREA
                     LKPDGRLVLLEYRAEDPNVPILPEHKMTKAQVKLEVEHEGFKQSRVYDDLPWQHLFVF
                     AMP"
     gene            complement(4013..4369)
                     /locus_tag="AC16CY_02245"
     CDS             complement(4013..4369)
                     /locus_tag="AC16CY_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352625.1"
                     /translation="MKLSVLVLGLFLLAAPAFAADVDGKWSGTIDTPNGAFPVSFMFK
                     ADGATLTGSQLGMDGMEIPIKDGKVDGTMITFLVSLDFGGMPFELSYKGEVSPSEIKL
                     TAEAFGMPFEFTVKKQ"
BASE COUNT          732 a         1663 c         1594 g          940 t
ORIGIN      
        1 gagttcggcg ccgacatcgt cggcatctgc gagatcgagc cgagcgacgt gtaccgcggc
       61 cgaacggtga ccgacaaata cgccgtcgcc gtgggccaga gaatgcgatg gcgggagttc
      121 caggtcgtcc cctcgcgcga atccgcgatc gagtgcctcc gcgtgtactt cacgctcggc
      181 gagtcggtca tccagctcgc cgctcacatc cgctcactcg gctacgcgtg caaggtggag
      241 cacccgatcg gcgacagcga cctgctgcac atcccgatcg gcctcaaggc cgggttcggc
      301 gagttggggc ggcacggctc gatcattcat ccccggctcg ggccgttgtt tcgcatgggc
      361 agcgtgatca cgtcgatggc gctcgagatc gaccatccga tcgatgccgg catcgcccgg
      421 ttttgcgatt cgtgccgggc gtgcaggaag ttctgcccgc cgcaggcgat tcccgacgag
      481 cgaagccctg aagccggcag agaccatctg ggctacgacc gctacgtggt cgatacggga
      541 cgctgctttc cctacttcgc gaagtattcc tactgctcga tctgcctgcc ggtctgcgcc
      601 tacaaccaca aggagtgggc ccgggacttc gagggcttcc agacgaagat cttcccgaaa
      661 gtcatcatgc tcgaaccgcc tccgccggca gacctgccgg ccggcgggcc gtgtcacgac
      721 taccccagga tcaaccgctc gtgaaggcgg cccgcctcgg cgcctggtcc gcgaccgcga
      781 tggtcgtgag ccaggtggtc ggcgtgggaa tcttcctgac gccggcgacg atgatgcgca
      841 cgatcggcag cgcgcccgcg gctctcgcgg tctgggcggt gatgggggcg ctcagcgcgg
      901 ccggcgcgct ctgctacgcc gaactgtcca cgcgcttccc aaaggcgggc ggtggctacg
      961 tattcctccg cgaggcgttc ggtccgcgct gcgcgtttct ttacgggtgg atgtcgctgc
     1021 tcgtggtcga tccaggcctc accgctgcgc tcggaatcgg gttcggccag tatctgctgg
     1081 cagcagttgg cgggccgcag gacctggtgc cgttcgtggc gattggggcc atcgtcctgt
     1141 tcggttcgct gacactcgtg ggggtgaacg cgagttcgcg tgtcatgcaa tggaccgcgg
     1201 ccgccaagct ggcgatcgtc gcgctgctcg tccttgctgc ccttctgcag acgtcgagcc
     1261 tggcgtcgcg ctggacggcc gctgcgcccg ccacgcttac acccgacgct ctcgcggcgt
     1321 cgcttgtcgc cgcgttcttc gcgttcggcg gctggtggga gttcggccgg atgagcgagg
     1381 aggtcgcgtc gccgaggcgg acgatgccgc gcgcgctcgt cggcggactc gcgctcgtga
     1441 cggggatgta cgcactggtc agcatggcgt acacgcttgc cgcgcccggc ggggtgcaga
     1501 cgaccaccga ggcgttcgtg gcagcggtgg gctccgcgct tttcggcgag gcagccgggc
     1561 gcctgctggc agtcatggtc gtcgtcgccg tggcgggaag cctggccgcg acactcctgg
     1621 gcgccccgcg cgtgtacctc gccatggcgc gcgatcgatt gttcccgcag cggctggcac
     1681 ggttcgaccc cgatcgcggc accgtgccgg gctccacgat cgtgcaggtc gtcctggcgt
     1741 cggcgctggc gctcgtcggc tcgttcgggg acgtcctcgg ctacttcgtg ccgtgcgcgg
     1801 tgttcttcct gggcctgtcg gccgcctcgg tgctggtact gccgcggccg gaggcccgcg
     1861 ctgacgtctt cgtcgcaccg ttccacccgc ttccaatcgt gctcttcctc gtgatggtct
     1921 cgtccgtgct cgtgctgttc gtgctcggcc agccgcgcca gactctcctc ggcgcactgg
     1981 tcgtcctgct cggcgtgccc gtctcgtggc tcgtcgtccc gcgacgctcc agtcgtggtt
     2041 agccgcatcg cacggctgcc gtgctagcat ccgttgattc gacgtgcgcg cccggcagcg
     2101 cggcgcgaag acgaaagggt ctccagtgtc caggcgaagc gtcgttgccc ttctcatgtt
     2161 tctgttgccg tcggcttccg ccgccgccga tctcaggatc accgacagcc ggggcgcgga
     2221 agtcctgctc accggcgcca gcatcgacta cagcggcttc gtggccgagg acaaggagac
     2281 gcaaggggtc cgcgtactcc agggcgaggg acgcgtcacc gtcaaatgga ccgacgtcga
     2341 gagtctcaaa gtcgtccggg tggacgagtc ggtgaggccg tctcgcatcg agatcgagat
     2401 cgtgttgaag aacgggaaga aagtgccggc ggcgcttcat cgacagggcc agatgaagct
     2461 gctcgggaag tcggacctgg gagactactc gatcgagctc gacaaggtcc gctcgatcgt
     2521 gccagtacac tagtggtccg tatacgctga agtagtacca gtcaccacga gacttcgacc
     2581 gcgatttccc ggccgcgaga acggagcggc cggtagtgat tcacgaagga cataccacta
     2641 gatgttcgtc gggcatctcg ccctcgccct cggaaccagg tacgtcgagc ccagagtgcc
     2701 tctcggcgcc gccgtagccg ccagttacgg cctcgacctg ctctggcccg tgctgctgct
     2761 gaccggcgcg gagtcggttc gcgtccaggc gggcgacaca gcgttcacga atctctcgtt
     2821 cacctcgtac ccctggagtc acagcctgct cgtcgtcgcc ggatggtccg ccctgacgat
     2881 gatcctggcg cagcgtgtgt acggctcggc acgcattggc aggttcctgg gcgcgctggt
     2941 cctcagtcac tgggtgctcg acgtcgtcac ccatcgcccg gacttgccgc tgtggcccgg
     3001 gggcccggtc gtaggcctcg ggctgtggaa ctcgatcgcc gccacgatcg tcgtcgaggg
     3061 cgcactactg gcggccggac tgtggctcta tcttcgcgcg agcgcggcac gcgatcagat
     3121 tggacgcctg gccctgacgg cactcgtcgc cgtgacttcg ttgatctgga tctcgcagcc
     3181 ctggtctcct ccgcctccga gcgcggccgc ggtggcgtgg ggtgcgttga tcctgtggct
     3241 tctccccccg tgggcagcct ggatcgacgc gcatcgcgcc tcgcgcacgt aatcgacgac
     3301 gtcacggcat cgcgaagacg aacaggtgct gccacggcag gtcgtcgtag acgcgtgatt
     3361 gcttgaagcc ctcgtgctcg acttccagct tgacctgcgc cttggtcatc ttgtgctcgg
     3421 gcaggatggg cacgttcggg tcctcggcgc gatactcgag gagcaccagc cggccgtccg
     3481 gcttgagcgc ctcgcggatg cgctgcagca tccgctgcgg ctgggaaaac tcgtgataga
     3541 cgtccaccat gatcgccagg tcgatgctcg cggggggcag cttcggatcg tcgatcgtcc
     3601 cgagtaccgg gacgacgttg tcgatcttgg cgcgggcgac gttctgctga atcaacgcga
     3661 tcatcccggg ttggatgtcg gacgcgtaga ccttgcccat cgggcccacg atcctggcga
     3721 gcctgatcgt gtagtacccg gacccggctc cgacgtcggc cacggttgac ccgcgcctga
     3781 tacgcaggag cctgagagcc agatccggat cttcttcccg ttcacgctcg cgtcgctcga
     3841 gccacggcgc tccctcgacg ctcatcgtcc gcgcgtacac gcggccgctg accgggtgca
     3901 cacccggctg ctgtgccgcg agcgcaatcg agaacgcgag tacgccgagg acggagatgg
     3961 ctttcacgct gccccctgcc cctgcccctg ccccctgtcc cctgccccct tcttactgct
     4021 tcttcaccgt gaattcgaac ggcatgccga acgcctcggc agtcagcttt atctctgacg
     4081 gcgagacctc gcctttatag gaaagctcga acggcatccc gccgaaatcg agggacacga
     4141 ggaacgtgat catcgtgccg tccactttgc cgtccttgat cgggatctcc atgccgtcca
     4201 tcccgagttg ggatccggtc agcgtggccc catcggcctt gaacatgaac gacacgggaa
     4261 aggcgccgtt cggcgtatcg atcgtaccgc tccacttgcc atccacgtcg gccgcaaacg
     4321 ctggcgccgc cagcaggaac aatccgagca ccaaaactga aagtttcata gacgaatctc
     4381 ctggaccccc tgcacaaccg actgcattga cctgttcgat gcacgcgcgg atcgccttgc
     4441 tcggcgtttt cgcacaacgg ccggggtgac taattgtaaa caggtgtcag aaaagcgtca
     4501 cgtcggcccg gggcacgaaa ggccagacac ttcaaaaacc gcccaaatcc tcgtaccggc
     4561 aactgggcac gacgtttgct cctgctcgat aacggcggtg agccttcagg gccacaccgc
     4621 cctccactga ccgctttgca ttggccgtat gtgccaggta aggcggtcag cgtgggtcgt
     4681 tcccgcccga cgtcctcgcg tttcccccgc ctccgcgtat ttcgatgcca cagctgttcg
     4741 gatcggggcg cgccagcgtt atcccatgaa agccactcgg gcgcgttgcg acatcctgat
     4801 gccaaaactc ggtgactcga aatcatgcgg cgtctgcggc ggggtaagca ttctccgtct
     4861 gcttcagccc tcagtcgcga cgctggggtg gattgagagt cgctcgattc cttacgacgt
     4921 tccccctct
//
LOCUS       DBNGSH010000101         1944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3912589, whole genome shotgun sequence.
ACCESSION   DBNGSH010000101 DBNGSH010000000
VERSION     DBNGSH010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3912589"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="AC16CY_02250"
     CDS             complement(<1..613)
                     /locus_tag="AC16CY_02250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3352626.1"
                     /translation="MRPWALPLLRCLECGGAAIETTLEHLRCASCGTTYARRGDIVDF
                     LYRPHPAIVREREAVHTLDREAGAPPDWMAAVLKRVDRGEVTEADMASSAHIRAIAES
                     RAQVLELLAQEPIARGSTVLEIGADVGWASSILLQAGCRVIATDVTDHLQLAASGDSP
                     DLCRLFADMNRLPLADATADVVFAASCVHHSWDLGRTFREIARV"
     gene            873..>1944
                     /locus_tag="AC16CY_02255"
     CDS             873..>1944
                     /locus_tag="AC16CY_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352627.1"
                     /translation="MTRMQRRMTLGILAIIGAVWFTSSPAGTQAPAARANATPAAGAK
                     TPWGDPDLQGTWTSEAELSVPFERAREHGERQTLTDQEFAARLSQTQKQLDSDNSDFD
                     LETADRSTAGQVGSATSPPPHWLERRKTSRRTSLVIDPPDGRIPPMTPEGQQRARGAR
                     GTFLNTGFRGPEDVGLWERCITRGLPGVIFPTVYNANTRIVQAPGMVAITYEMIHDTR
                     IIPLDGRPHVSQDVRHYFGDSRGHWDGDTLVVDVTNFTEKSNYRGSRDTLHLVERFTR
                     AGNTLLYSVTVDDSKTWTKPWTAALTLEQSDGLYEYACHEGNYAMRNILSGARAADRT
                     KEWAGGRGQGAVGQGAVGPGAGG"
BASE COUNT          348 a          677 c          654 g          265 t
ORIGIN      
        1 gtacgcgggc gatctcgcgg aacgtacgcc cgaggtccca cgagtgatgc acgcacgagg
       61 ccgcgaacac gacgtcggcc gtcgcatcgg cgagcggcag ccggttcatg tcagcgaaca
      121 gccggcacag atccggcgag tcgccggagg ccgcgagctg cagatggtcg gtcacatcgg
      181 tcgcaatcac gcggcacccg gcctggagca ggatggagct cgcccagccg acgtcggcgc
      241 caatttcgag cacggtggat ccgcgtgcga tcggctcctg cgcgagcagc tccaggacct
      301 gcgcgcgcga ttcggcaatc gccctgatgt gcgccgaact cgccatgtcc gcctcggtca
      361 cttcaccgcg atccacgcgc ttcaggaccg ccgccatcca gtcgggcggc gcgccggcct
      421 cccgatccag cgtgtgaacg gcctcgcgct cgcgcacgat ggctgggtgc ggccggtaca
      481 ggaagtccac gatatcgcct cgtcgcgcgt acgtggtccc gcacgacgcg caacgaaggt
      541 gctcgagcgt cgtctcgatc gcagcgccgc cgcactccag gcagcgaaga aggggaagcg
      601 cccagggtct catctccggc cctagaatat cccccacatc tgctacgctc tttccgtcac
      661 ggtccggctg ccttcgactg cgctcaggcc aggaaagccg gacactacgt tgatcacggt
      721 ccggctgaag ccggacacta cgtcagtcac ggtccggcta aagccggaca ctacgttgat
      781 cacggtccgg ctaaagccgg acactacgtc agtcacggtc cggctgaagc cggacactac
      841 gtcggtcacg gtctcgtcac ggggaggctc cgatgacccg catgcagcgg cggatgacgc
      901 taggtatcct cgcaattatc ggcgcggttt ggttcacctc gtcgccagcc ggcacgcagg
      961 cgccggccgc gcgcgcgaac gcgacacccg ccgccggcgc gaagacgccg tggggcgacc
     1021 cggacctgca aggcacgtgg acgagcgagg cggagctcag cgtccccttc gagcgggccc
     1081 gcgagcacgg cgagcggcag acgctgaccg accaggaatt cgcggcgcgc ctgtcgcaga
     1141 cgcagaagca actcgacagc gacaactcgg acttcgatct cgaaacggcc gatcggtcca
     1201 cggccggcca ggtcggctcc gccacctcgc ctccaccgca ctggctcgag cggcggaaga
     1261 cctcgcgccg cacgtcgctc gtgatcgatc cgccggatgg ccgcatcccg ccgatgacgc
     1321 ccgaagggca gcagcgtgca cgcggcgcgc gcggcacgtt cctcaacacc ggcttccgcg
     1381 gacccgagga cgtggggctc tgggaacgct gcatcacgcg cggcctgccg ggggtgatct
     1441 tccccaccgt ctacaacgcc aacacccgca tcgtccaggc accgggaatg gtcgcgatca
     1501 cctacgagat gatccacgac acgcgcatca ttcctctcga cggccggccg cacgtgtcgc
     1561 aggacgttcg gcactacttc ggcgactcgc gcgggcactg ggacggcgac acgctggtcg
     1621 tggacgtgac caacttcacc gagaagagca actaccgcgg atcgcgcgac acgctgcacc
     1681 tggtcgaacg cttcacgcgt gccggcaata cgctgctgta ctcggtcacg gtggacgatt
     1741 cgaagacgtg gacgaagccg tggacagcgg ccctcacgct cgaacaatcg gatggcctct
     1801 acgagtacgc gtgccacgag gggaattacg cgatgcggaa catcctcagc ggggcgcgcg
     1861 cggcggaccg tacaaaggag tgggcagggg gcagggggca gggggcagtc gggcaggggg
     1921 cagtcgggcc gggggccggg ggaa
//
LOCUS       DBNGSH010000102         4723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3403036, whole genome shotgun sequence.
ACCESSION   DBNGSH010000102 DBNGSH010000000
VERSION     DBNGSH010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3403036"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /gene="hutU"
                     /locus_tag="AC16CY_02260"
     CDS             complement(<1..1619)
                     /gene="hutU"
                     /locus_tag="AC16CY_02260"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR3352628.1"
                     /translation="MTIGTRTIRAPRGSAISCKGWPQEAALRMLMNNLDPDVAERPED
                     LVVYGGSGKAARSWDAFDAIVSELRHLDDDETLIVQSGKPVAVFRTHAAAPRVLIANA
                     LLVPAWATWETFRDLEHRGLTMYGQMTAGSWIYIGTQGILQGTYETFAAAARRHFGGS
                     LSGRVVVSAGLGGMGGAQPLAATMNGGAALIVEIDPERIERRLATRYLDEATSDLDEA
                     LERVESWRRDGRARSIGVEANAADVLPELVGRGVTPDVLTDQTSAHDALNGYVPHGMT
                     LADARRLRDRDPDEYVARATSSMGRHVEAMLALKARGAVAFDYGNNIRAQALSAGVRN
                     AFDIPGFVPEYVRPLFCQGQGPFRWVALSGDPDDIRATDEAALEMFADNPALCRWIRM
                     AQERVAFQGLPARICWLGYGDRARFGLRLNELVRSGRVSAPIVIGRDHLDTGSVASPN
                     RETEGMLDGSDAIADWPILNALVNASAGATWVSVHHGGGVGIGYSLHAGMVVVADGTE
                     EADDRLERVLTCDPGLGVARHADAGYAEAIDA"
     gene            complement(1710..3224)
                     /gene="hutH"
                     /locus_tag="AC16CY_02265"
     CDS             complement(1710..3224)
                     /gene="hutH"
                     /locus_tag="AC16CY_02265"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR3352629.1"
                     /translation="MIVLDGHSLTLDDLVAIAVERAPVSLTDDARARVRAARAVVDEA
                     AGGGQAVYGVNTGFGSFADVRIPPGSLGELQVNLLRSHAAGVGEPLPVPVVRATMALR
                     ANVLAKGFSGIRLETLDRLLDLLNRGVHPVVPSRGSVGASGDLAPLAHLALVLIGEGE
                     AWHDEARQSGADALGRAGLAPLTLHAKEGLALINGTQPSTALLGLALSGTIQLVKAAD
                     ITAAMSIDGLQGSTRPFDARIHAARGVAGQAVSAENLRKLLEGSPINASHANCGRVQD
                     AYSMRCTPQVHGAAREAAQFALRAFHAEANAATDNPMVFVDTREMISGGNFHGAPVAL
                     AADTLAIGLAQLATISERRSDRLVNPASSGLPAFLTRHGGLQSGLMMAQVTAAALTSE
                     LKTLAHPASVDTIPTSGNKEDHVSMSMGAALKASRAVALARDVVAIELLCACQAIDLL
                     EGLTTSAPLARVHRLVRACVPTLDADRPPSHDIVAIARLIDTGEIDRACAMKVN"
     gene            3299..4609
                     /locus_tag="AC16CY_02270"
     CDS             3299..4609
                     /locus_tag="AC16CY_02270"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR3352630.1"
                     /translation="MANRNIAILGAQWGDEGKGKIVDVLTPHFSAVARYQGGHNAGHT
                     VYVKGKKFVLHLIPSGILHPGVTCLIGNGVVVDPQALFAEVDELQRAGVSVDGRLLVS
                     EKAHVILPYHRELDVLSEARRGERKIGTTSRGIGPAYEDKIGRRGIRVRDLLGDRQAL
                     ADDVRENVSARNRMIKDSTLDWKPVLDQVVAAGERMRPWVADVSLSLHRLMAEGKTVL
                     FEGAQATLLDIDHGTYPFVTSSNASVGGVCTGLGVPPRAIGGVLGVAKAYTTRVGEGP
                     LPTEIAGELADRLRESGQEYGASTGRPRRCGWFDAVVVRYSARINGLDAVALTKLDVL
                     DGLPEVLICTGYRTGNGELSEFPADLRLLAGAEPVYERLPGWSTPTKGATRIEDLPAE
                     ARRYVRRLEEVTGVGCSIISTGSDRSETIVAPGSVVEGWLGSSR"
BASE COUNT          741 a         1623 c         1616 g          743 t
ORIGIN      
        1 gcgtcgatcg cctcagcgta ccccgcgtcg gcatgcctcg cgacgcccag gcccggatcg
       61 caggtcaaca cgcgctccag ccgatcgtcc gcctcttccg tgccgtcggc cacgaccacc
      121 atgccggcgt gcagcgagta gccgatgccg actccgccgc cgtgatgcac cgacacccac
      181 gtggcgccgg ccgacgcgtt gacgagcgcg ttcaggatcg gccaatcggc gatcgcatcg
      241 ctcccgtcga gcatcccttc ggtctcgcgg ttcggcgagg cgaccgatcc cgtgtccaga
      301 tggtcgcggc cgatgacgat cggcgcgctc acgcgccccg accgcaccag ctcgttcaac
      361 ctcagtccga agcgcgccct gtcgccgtag ccgagccagc agatccgcgc cggcaatccc
      421 tggaacgcga cgcgctcctg cgccatgcgg atccatcgac acagtgcggg attatccgca
      481 aacatctcga gcgcggcctc gtccgtcgcg cgaatgtcgt cgggatcgcc tgacagcgcc
      541 acccaccgga acggcccctg accctggcag aacaacggcc gcacatattc cggcacgaag
      601 cccggaatgt caaacgcatt tctcacgccg gcggaaagcg cctgcgcgcg tatgttgttg
      661 ccgtagtcga acgccaccgc gccgcgcgcc ttgagcgcga gcatcgcctc gacatgccgg
      721 cccatcgacg acgtcgcgcg cgccacatac tcgtcgggat cgcggtcgcg cagccggcgc
      781 gcatcagcaa gcgtcatgcc gtggggcaca tagccgttga gcgcatcgtg cgccgacgtt
      841 tgatcggtca gcacgtccgg cgtcacgcct cggccaacga gctccggcag gacgtcggcc
      901 gcgttggcct ctacgccgat cgatcgcgcg cgcccgtcgc gacgccagga ctcgacgcgc
      961 tcgagcgcct cgtccagatc cgaggtggct tcgtcgagat accgcgtcgc cagccgccgc
     1021 tcgattcgct ccgggtcgat ttcgacgatc agcgcggcgc cgccgttcat cgtggcggcc
     1081 agcggctggg cgccgcccat gccgccgagc ccggccgaca ccacgacccg gccgctgagg
     1141 ctcccgccga aatgccggcg cgccgcggcg gcaaaggtct cgtaggttcc ctgcagaatc
     1201 ccctgtgttc ctatgtagat ccagctgccg gccgtcatct ggccgtacat cgtcagcccg
     1261 cgatgctcga ggtcgcgaaa cgtctcccac gtcgcccagg cgggtacgag gagggcgttg
     1321 gcgatgagca cgcgaggcgc ggcggcatgc gtgcggaaca ccgcaacggg cttccccgac
     1381 tggacgatca gcgtctcgtc atcgtcgagg tgacgaagct ccgacacgat cgcatcgaag
     1441 gcatcccagc tgcgcgccgc tttgccggag ccgccgtaga cgacgagatc ctcgggccgc
     1501 tccgccacgt ccggatcgag gttgttcatc agcatgcgaa gcgcggcttc ctgcggccat
     1561 cctttgcagg agatcgccga gccgcgagga gctcgtatcg tccgcgtgcc gatcgtcatg
     1621 ttggagaggg cgcagcgtta cagcctgctg cggagatcag aaggtaagaa tcgcgcgtgc
     1681 gcttgtcaat cgatttgaga aaattttttt cagttgactt tcatcgcgca agcgcgatcg
     1741 atctcgccgg tatcgatcag gcgcgcaatt gcgacgatgt catgcgaggg tggacgatcg
     1801 gcgtcgagcg tcggcacgca ggcgcgcacg agtcgatgca cgcgcgcgag cggcgccgac
     1861 gtcgtgaggc cctccagcag atcgatcgcc tggcacgcgc acagcagttc gatcgccacg
     1921 acgtcgcgcg cgagcgcgac ggcacgcgac gccttgagcg ccgcgcccat gctcatgctc
     1981 acgtgatctt ccttgttgcc cgaggtcggg atcgtgtcca cgcttgccgg atgagccagc
     2041 gtcttcagtt cggaggtcag cgccgccgcg gtgacctgag ccatcatgag gcccgactgc
     2101 agacctccgt gccgcgtcag gaacgcaggc agcccgctgc tcgcgggatt cacgaggcga
     2161 tcggatcttc gctcgctgat cgtcgccagc tgcgcgagcc cgatcgcgag cgtgtcggcg
     2221 gcgagcgcga cgggagcgcc gtggaagttg ccgcccgaga tcatctccct cgtgtccacg
     2281 aacaccatcg ggttgtcggt cgcggcgttg gcctccgcgt gaaacgcgcg gagcgcgaat
     2341 tgcgcggctt cccgggccgc gccgtgcacc tggggcgtgc accgcatcga gtaggcgtcc
     2401 tggacgcggc cgcagttggc gtgggacgcg ttgatcgggc tcccctcgag caatttcctc
     2461 aggttttcgg ctgaaaccgc ctggccggcg acgccgcgcg cggcgtggat tctcgcgtcg
     2521 aacgggcggg tcgagccctg cagcccgtcg atgctcatgg ccgcggtgat gtcggcagcc
     2581 ttgacgagct ggatcgtgcc cgacagcgcc agccccagga gcgccgtcga gggctgcgtt
     2641 ccgttgatga gtgcaagccc ttccttggcg tggagcgtca gcggcgcgag ccccgcgcgg
     2701 ccgagcgcgt cggcgcctga ctgccgggcc tcgtcgtgcc acgcttcgcc ttcgccgatc
     2761 aacacgagcg ccagatgggc cagcggcgcg aggtcgccgc tcgcgcccac cgatccgcga
     2821 gacggcacga cgggatgcac gccgcggttg aggagatcga ggagccgatc gagcgtctcg
     2881 agccggatgc ccgagaaccc tttcgccagc acgttcgcgc gcagggccat cgtcgcgcgc
     2941 acgacgggaa ccggaagcgg ctcgccgacg ccggccgcgt ggctgcggag aaggttcacc
     3001 tgcagctcgc cgagcgagcc cggtggaata cggacgtcgg cgaagctgcc gaagccggtg
     3061 ttgacgccgt agacggcctg cccgccgccg gccgcctcgt cgacaacggc ccgcgcggcc
     3121 cgcacgcgcg cgcgcgcatc gtcggtcagc gacaccggag cgcgttcgac ggcgatcgcg
     3181 acgagatcgt cgagcgtgag cgagtggccg tctagaacga tcacgggcgt gtgtcgtgta
     3241 ttccgtctag tgtacgtcgt tcgcgcgctt tgtgcttcgt ggttgaatag tagccgcaat
     3301 ggcgaaccgg aacatcgcga tcctcggcgc gcagtggggc gacgaaggca aggggaagat
     3361 cgttgacgtc ctcacgccgc acttttcagc cgtcgcccgc taccaggggg ggcacaacgc
     3421 cgggcatacg gtgtatgtca aggggaagaa gttcgtcctg catctgattc cgtcgggcat
     3481 cctgcatccg ggcgtgacgt gtctcatcgg caacggcgtc gtcgtggatc cgcaggcgct
     3541 gtttgccgaa gtagacgaac tgcagcgggc cggcgtgtcc gtggacggcc gcctgctggt
     3601 cagcgagaag gcgcacgtca tcctgccgta tcaccgggag ctcgacgtgc tgtcggaggc
     3661 gcgccgcggc gaacggaaga tcggcaccac ctcgcgcggt atcgggccgg cctacgagga
     3721 caagatcggg cggcgcggca tccgcgtgcg ggatctgctg ggagaccgcc aggcgctcgc
     3781 cgacgacgtg cgcgagaacg tgagcgcccg caaccggatg atcaaggact cgacgctcga
     3841 ctggaagccc gtgctcgacc aggtcgtcgc ggcgggcgag cggatgcgtc cctgggtggc
     3901 cgacgtgtcg ctgtcgctcc accggctgat ggccgaaggg aagaccgtgc tgttcgaggg
     3961 agcgcaggcc acgctgctcg acatcgatca cgggacctat cccttcgtca cctcgtcgaa
     4021 tgcgtcggtc ggcggcgtgt gcaccggcct cggcgtaccg ccgcgcgcca tcggcggcgt
     4081 gctcggcgtt gcgaaggcgt acacgacgcg ggtgggcgag ggtccgctgc ccaccgagat
     4141 cgccggcgag ctggccgatc ggctccgcga aagcggccag gagtacgggg catcgaccgg
     4201 gcgtccccgg cgctgcggct ggttcgacgc cgtcgtcgtc cgctattcgg ctcgcatcaa
     4261 cggcctcgac gccgtggcgc tgacgaagct ggacgtgctg gacggactgc cggaagtgct
     4321 catctgcacg ggctacagaa ccggcaacgg tgagctctcg gagttcccgg cggatctgcg
     4381 gttgctcgcg ggcgccgagc cggtctacga acgcctgccg gggtggtcga cgccgacgaa
     4441 gggcgcaacc cgcatcgagg atctgcccgc cgaagcgcgg cgctacgtgc gccggttgga
     4501 ggaagtcacc ggagtcggct gctcgatcat ctcgaccgga tccgaccgga gcgagacaat
     4561 cgtcgcgccc ggatcggtgg tcgaaggctg gctcggatca tcgcgctgag tcacgtcgcc
     4621 ttccactccc gattcgccat ttccgagacg gacgtcaacc acagagaccc agaggacacc
     4681 gagaccatca gctctgcgtc ctctgtgact ctgtggttaa cgg
//
LOCUS       DBNGSH010000103         4678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1195452, whole genome shotgun sequence.
ACCESSION   DBNGSH010000103 DBNGSH010000000
VERSION     DBNGSH010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1195452"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC16CY_02275"
     CDS             complement(<1..217)
                     /locus_tag="AC16CY_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352631.1"
                     /translation="MRSVILLLLCFVGVACDSTSPTADLTGTWSGQVGLPTSGSALRV
                     TWLATQAGTALTGPATLVKPAVNVPATG"
     gene            complement(227..1489)
                     /locus_tag="AC16CY_02280"
     CDS             complement(227..1489)
                     /locus_tag="AC16CY_02280"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3352632.1"
                     /translation="MIIGGGFGGLSAARVLRNADVRVTLLDRRNHHVFQPLLYQVATA
                     TLSPGDIAAPIRWILRRMSNVRVLLGEASRVDIASRRVELTDGATLDYDWLIIATGST
                     HAYFGHDDWEKDAPGLKTLEDAIGIRRRILIAFERAEREEDAARQRELLTFVLVGGGP
                     TGVELAGTLAEIARQTLRHEFRSIDTTRARIVLVEAGPTILPTFPEALRRAARESLRR
                     LGVEIREGTAVTSVDQHGVIAGGERLDAGTVLWAAGVAASPLVRSLGVPLDRAGRAIV
                     EPDLSIPGHPDVFVVGDAAAFLHQGGTLLPGVAQTAMQGAAHAARTILRRLRGSAPRP
                     FVYRNYGSMAIVGRRAAISDLRWVRFSGVVSWFAWLFLHIFMLIGFRNRVVVLFEWAV
                     AYITFQRGARLITNDPGSRGREDGAVRG"
     gene            complement(1504..3717)
                     /locus_tag="AC16CY_02285"
     CDS             complement(1504..3717)
                     /locus_tag="AC16CY_02285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3352633.1"
                     /translation="MKPLTGQLDRRSFLRVSALAGGGMMVAAYLEPVARVLAQGPGQT
                     FVPNAFIRITPDNIVTIVSKNPEIGQGIKTSLPMILADELGVPWSSVRIEQADLDETK
                     YGRQNAGGSTATPTNWDPLRQVGAAVREMLVASAAQEWGVPASECVAADGRVSHRATG
                     RTQLYGALASHAATMTPPDLQSVRLRPASEYKIIGKFTGGVDNARIVTGQPTFGIDFT
                     LPNMLWAVYEKCPVFGGKVVSANYDQVKAMPGVRHVFAVEGTNDLRGLMPGVAIVADS
                     WWQAKTARAKLQVQWNQGATAQQSSEGFARRAREISTEKPGFPLRVDGDTTAALQGAA
                     KVVEAAYDYPFISHAPLEPQNCTAHWRDGRMEIWAPTQTPANGRQLVSSALGIPESAI
                     TIHIQRAGGGFGRRLTNDYMGEAAAITKQIGVPVKVLWTREDDMGHDHYRPGGFHFLK
                     AGVDASGRLVAWRNHFVSYGDNARGEQGFSNSANIPGVEFPARFIPNYDFQATLMALG
                     VPTGAHRAPRSNAFSFVFQSFIDEIALAAGKDPIAFRLELLNAQVYPEPQQGGNGFDA
                     ARMRGVLEAVRDRSGWGKQQLPAGTAMGVGFQYSHRGYFANVAEVTVDASNRVKVNKV
                     WVAGDVGRQIVNPSSAVNQSQGAVVEGMSHLMGWEISIQGGKAVQNNFHQYPTVRMSQ
                     APDVDVHFVVTDNPPTGLGEPALPPTVPALANAIFAATGKRIRSLPISKQGFRWA"
     gene            3849..4295
                     /locus_tag="AC16CY_02290"
     CDS             3849..4295
                     /locus_tag="AC16CY_02290"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HIR3352634.1"
                     /translation="MRVSQKLVIVAALVVLAYPVALGAQAGETYTARLGWVPTTPADR
                     PNVIGKGSATATLSGRRLSISGSFDGLAAPATIARLHRGIAKAARGPAISDLTITQAA
                     SGSLSGAVDLTAEQLEDLKQGKLYIQVHSAKGVAPDGSTLWGWLLK"
     gene            4335..>4678
                     /locus_tag="AC16CY_02295"
     CDS             4335..>4678
                     /locus_tag="AC16CY_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3352635.1"
                     /translation="MGPKNSSLLVAFAVLAATGVLLTGQSPAAGPFTVAQATAGRQAY
                     EANCAGCHGNNLDGVPPLSGDAFMGPWRARTTRDLFGLVRTTMPSDNAGGLPEGTYVN
                     IIAYILQRNGVAP"
BASE COUNT          770 a         1587 c         1566 g          755 t
ORIGIN      
        1 taccagtcgc cggcacgttc accgcgggct tgacgagcgt tgctggaccg gtgagggccg
       61 ttcccgcctg cgtcgccaac cacgtcaccc ggagcgccga tcccgacgtc ggcaggccga
      121 cctgccccga ccaggtgccg gtcagatcag ccgtcggaga cgtggagtcg cacgcaacgc
      181 ccacgaagca cagcaacagc aggattacag atcgcatata ccaatcctac ccccggaccg
      241 cgccgtcctc tctcccccgc gatcccggat cgttggtgat caatcgggcc ccccgctgaa
      301 acgtgatgta ggccacggcc cattcaaaca gcacgacgac gcggttgcgg aagccgatga
      361 gcatgaagat gtgaaggaac agccacgcga accacgagac aacgccggaa aacctgaccc
      421 atcgaaggtc gctgatcgcc gcacgccggc cgacgatcgc catgctgccg taattgcgat
      481 agacaaacgg ccgtggcgca ctgccgcgca gccgccgcag gatcgtacga gccgcgtgtg
      541 cggcgccttg catggcggtc tgcgccaccc ccggcaggag cgtgccgccc tgatggagaa
      601 acgccgccgc gtcgccgacg acgaaaacgt cgggatggcc ggggatcgac agatcgggct
      661 ccacgatcgc gcggccggct cgatcgagcg gaacgcccag cgagcgcacg agcggcgatg
      721 cagccacgcc cgccgcccac agcactgtgc cggcgtcgag gcgttcgccc ccggcaatca
      781 cgccatgctg gtcaaccgac gtgacagccg tgccttcccg aatctcgaca cccaggcggc
      841 ggagcgactc gcgagccgcg cggcgaagcg cctcggggaa cgtcggcagg attgtcggac
      901 cggcctcgac gagcacgatt ctggcgcggg tggtgtcgat cgatcggaat tcgtgacgca
      961 gcgtctggcg ggcgatctcg gcgagcgtgc ccgcgagctc gacgccggtg ggaccgccgc
     1021 cgacgaggac gaacgtgagc agctctcgct gacgggcggc atcctcctct cgctcggcgc
     1081 gttcgaacgc aatcaggatc cggcgtctga tgccgatcgc atcttccagt gtcttcagac
     1141 cgggtgcgtc cttctcccaa tcgtcgtggc cgaagtaggc gtgcgtggat cccgtcgcga
     1201 tgatgagcca gtcgtagtcc agcgtggcgc catcggtcag ctcgacgcgg cgcgaggcaa
     1261 tgtcgacgcg actggcctcg ccgagcagaa cgcggacgtt gctcatgcgt cggaggatcc
     1321 acctgatggg agcggcgatg tcgccgggag agagcgtcgc ggtggcgacc tggtagagca
     1381 gcggctgaaa gacgtggtga ttgcggcggt cgagcagcgt gacccgcacg tccgcgttcc
     1441 gcagcactct ggctgcggac aggccgccga agcctccgcc gatgatcacg acgtgtggaa
     1501 cggctacgcc cagcggaacc cttgcttcga gatcggcagc gaccggatcc gctttcccgt
     1561 cgcggcgaag atcgcgttgg ccagcgccgg aaccgtcggc ggcagcgcgg gctcgccgag
     1621 acccgtcggc gggttgtccg tgacgacgaa gtgcacgtcc acgtccggag cctgggacat
     1681 ccggaccgtt gggtactggt ggaagttgtt ctggaccgcc ttgccgcctt gaatggagat
     1741 ctcccatccc atcaggtggc tcatgccctc gacgacggcg ccctggctct ggttgacggc
     1801 gctgctcggg ttcacgatct ggcggccgac gtcacctgcc acccacacct tgttcacctt
     1861 gacgcggttc gaagcgtcga cggtgacttc agccacgttc gcgaagtacc cgcgatggct
     1921 gtactggaag ccgacgccca tcgccgtgcc ggccgggagc tgttgcttgc cccagccgga
     1981 tcggtcgcgc acggcttcga gcacgccgcg catgcgcgcc gcgtcgaaac cattgccccc
     2041 ctgctgcggc tcggggtaca cctgcgcgtt caacagctcg aggcgaaaag cgatcgggtc
     2101 tttccccgcc gccagcgcaa tttcgtcgat gaacgactgg aagacgaacg agaacgcatt
     2161 gctgcgcggt gcgcgatggg cgccggtcgg tacgcccagc gccatcagcg tcgcctggaa
     2221 gtcgtagttc gggatgaacc tggccgggaa ctcgacgccg gggatgttgg ccgaattgga
     2281 gaagccctgc tctccgcgcg cgttgtcgcc atagctgacg aagtgattcc gccacgcgac
     2341 gagacgcccg gacgcgtcca cgcccgcctt caggaagtgg aacccgcccg gccggtagtg
     2401 gtcgtgcccc atgtcgtctt cgcgcgtcca cagcaccttg accgggacgc cgatctgttt
     2461 ggtgatcgcc gccgcctcgc ccatgtagtc gttggtcagc cgacggccga agccgcctcc
     2521 ggctctctgg atgtggatcg tgatggccga ctccgggatg ccgagcgcgc tcgagacgag
     2581 ctggcgtccg ttggccggcg tctgcgtcgg cgcccagatc tccatccggc cgtcgcgcca
     2641 gtgcgccgtg cagttctgcg gctcgagagg tgcgtgcgag atgaacggat agtcgtaggc
     2701 ggcctcgaca accttcgccg cgccctggag cgccgccgtg gtgtcgccgt ccacgcgaag
     2761 cgggaacccg ggcttctccg tcgagatctc acgcgcgcgc cgggcgaagc cttcgctgct
     2821 ctgttgcgcc gtggccccct ggttccactg tacctgcagc ttcgcgcgcg ccgtcttcgc
     2881 ctgccaccag ctgtcggcga cgatcgccac gccgggcatc aggccccgaa ggtcgttcgt
     2941 gccttccacg gcgaagacgt gacggacgcc cggcatcgcc ttgacctgat cgtagttcgc
     3001 gctgacgacc ttgccgccga acaccggaca cttctcgtag accgcccaga gcatgttcgg
     3061 aagcgtgaag tcgatgccga aggtgggctg gccggtgacg atgcgggcgt tgtccacgcc
     3121 gccggtgaac ttcccgatga tcttgtactc gctggcaggc cggaggcgca ccgactgcag
     3181 gtcgggcggc gtcattgtcg cggcgtgcga cgcgagcgcg ccgtacagct gcgtacgccc
     3241 agttgcgcgg tgcgagacac gcccgtccgc agcgacgcac tccgacgcgg gcacgcccca
     3301 ctcctgtgcg gccgacgcga caagcatctc tcgcacggct gcgcccacct gcctcagcgg
     3361 gtcccagttg gtcggcgtcg ccgtgctgcc tcccgcgttc tgccgtccgt atttcgtctc
     3421 gtcgagatcg gcctgctcga tgcgcacgct gctccacgga accccgagct cgtcggcgag
     3481 gatcatcggc agagacgtct tgatgccctg gccgatctca gggttcttcg acacgatcgt
     3541 cacgatgttg tcgggcgtga tcctgatgaa cgcgttggga acgaacgtct gccccggacc
     3601 ctgcgcgagc acgcgggcca ccggttcgag gtaggcggcc accatcatcc cgccgccggc
     3661 cagggcggac acgcgaagga acgaccgccg atcgagctga cctgtcagtg gcttcatcgc
     3721 tactccagtc gtggggtcag accccatgag tcgagtcgtg tcatggggtc tgaccccagg
     3781 gatacaccgg ctagacgcta ggaagtataa gatacgccgc gttgggacgc tttctgtcga
     3841 ggtgaggaat gagggtcagc cagaaattgg tcatcgttgc cgcgctcgtg gtcctcgcgt
     3901 accccgtcgc gcttggggcg caggccggag agacgtatac ggccaggctt ggatgggtgc
     3961 cgacgacgcc ggcggatcgt cccaacgtga tcgggaaggg ctctgccacg gcgacgctgt
     4021 ccgggagaag actgtcgatc tccggatcct tcgacggcct ggcagcgccg gcgacgatcg
     4081 cgcggctcca ccgcggcatc gccaaggccg cccggggacc ggccatctcg gacctcacga
     4141 tcacgcaggc cgcgagcggg agtctctccg gcgctgtcga tctgacggcc gaacagctcg
     4201 aggacttgaa acagggcaag ctctacatcc aggtgcacag cgccaagggc gtcgcaccgg
     4261 acggcagcac gttgtgggga tggttgctga aatgactcag ggcaaaggtc atagggcaag
     4321 gtgaggtaac gacgatgggt ccgaagaaca gttctctgct cgtggcattt gcggtgctcg
     4381 cggcaacggg ggtgctgctc accggtcaat cgcccgccgc cggcccgttc accgtggcgc
     4441 aggcgaccgc cggccgtcag gcctatgagg ccaactgcgc cgggtgccac ggcaacaacc
     4501 tggatggggt gcctccactc tcgggtgacg cgttcatggg cccgtggagg gcgcgaacca
     4561 ctcgcgacct gttcggtctg gtgcgcacga ccatgccctc ggacaacgcc ggaggcctgc
     4621 cggaaggcac ctacgtcaac atcatcgcct acatcctgca gcgcaacggc gtcgcgcc
//
LOCUS       DBNGSH010000104         2896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4421941, whole genome shotgun sequence.
ACCESSION   DBNGSH010000104 DBNGSH010000000
VERSION     DBNGSH010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4421941"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="AC16CY_02300"
     CDS             <1..240
                     /locus_tag="AC16CY_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3352636.1"
                     /translation="FPRGVPGDAVRDKIAEVNQIISRLDDKKNVFYMDIGQKFLDDKG
                     VFLPESFRPDNLHPAAKGYDIWGQAVSAKLAELMK"
     gene            complement(209..1279)
                     /locus_tag="AC16CY_02305"
     CDS             complement(209..1279)
                     /locus_tag="AC16CY_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3352637.1"
                     /translation="MSRTRTGLAALLATLVAALSVVQAQPRGPAREIVPLTGDLYRAR
                     NGNWYAIFLVTRDGIILGDPINPAFASWLKDELGRRFTVPVRYVVYSHSHFDHAAGGA
                     VFADTARFVAHENMRRNMDGRYPHMPGDMIDRNRNGAIDPDEIDIPTKAAPGVCGMGP
                     GFFATIDRNKDGLVPPAELQGDIRPPDIVYSERMSIALGGRTVDLIHPGLNHSDDATV
                     MHFREERVVFATEFLADALVTTNPRSLPSTCSAFDRHPLSEWIRSYRTVEALDFDRVA
                     PGHGDMFDKQVVTETREYFEYLVAQVSAGMKSGRPLAELKETITLDRYKGWANYERLR
                     RMNVEAAYLNLTSTSSVPPASR"
     gene            1380..>2896
                     /locus_tag="AC16CY_02310"
     CDS             1380..>2896
                     /locus_tag="AC16CY_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3352638.1"
                     /translation="MGTTSETPVLIVGGGAAGSVLAMELARRGVEFRCIDRMPGPGRE
                     SRAIALHARTIELMDLVDTALSRRMLDRDLWCKGYVMHFLRGRERTEVRPGLDYTKVD
                     SRYNCILSHNQSETEGFIREYTRSRFGREVEYDTAFQDLTQDGDLVTARVTHGDRGDQ
                     EEFIRCRYLIAADGINSRVRRGLGLSVKGKDYKGSFFQNLDIHLKGFTDWRDYFHYCV
                     GTDHFLMVAPLPGGHFRLLLSDRGEAADPTLTPQQAFMRLLDQHYDGITMGEVVWHSK
                     WETWVRLADTFRQGNVFLAGDSAHVHSTTGGQGMNCCMQDAFNLGWKLGLVLKGYARP
                     TLLDTYEAERRPVAEQVIWAASSLHDIFMTHGKDIAQRTQTMFEPGYTEKVVNSCSGV
                     AYTYKDFIDKPPGLREIDGPAIGDRAPDVDFERGGTLFDRLRHTYFTLLAMPDGDLSP
                     IVERLGRRFGPVLDVEILPKSDALSARYGQSDGRLFLVRPDGYIAFKSCADEAGLL"
BASE COUNT          510 a          930 c          967 g          489 t
ORIGIN      
        1 ttcccgaggg gcgtgccggg cgatgcggtc cgcgacaaga tcgccgaggt caaccagatc
       61 atctcgaggc tggacgacaa gaagaacgtc ttctacatgg acatcggcca gaagttcctg
      121 gacgacaagg gcgtgttcct gcccgagtcg ttcaggcccg acaacctgca cccggcagcg
      181 aagggctacg acatctgggg ccaggccgtc agcgcgaagc tggcggaact gatgaagtag
      241 acgtcaggtt cagataggcc gcttcgacgt tcatgcggcg aaggcgctcg tagttcgccc
      301 atcccttgta gcgatcgagc gtgatggtct ccttcagttc ggccagcggc cggccggatt
      361 tcatgccagc cgatacctgc gcgacgagat actcgaagta ctcgcgcgtc tcggtgacga
      421 cctgcttgtc gaacatgtcc ccatggcccg gcgccacgcg atcgaaatcg agcgcttcga
      481 ccgtccggta ggaacggatc cactccgata ggggatgccg gtcgaacgcg ctgcaggtgc
      541 ttggcagcga ccgcgggttc gtcgtgacga gcgcgtcggc gaggaactcg gtagcgaaca
      601 ccacccgttc ctcccggaaa tgcatcaccg tcgcgtcgtc ggaatgattc agcccgggat
      661 gaatcaggtc cacggtgcgc ccgccgagcg cgatgctcat gcgctccgag tagacgatgt
      721 cgggaggacg gatgtcgccc tgcagctccg ccggcggcac gaggccgtcc ttgttgcgat
      781 cgatcgtcgc gaagaacccg ggccccatgc cgcagacgcc cggcgccgcc ttcgtcggga
      841 tgtcgatctc gtccgggtcg atggcgccgt tgcgattgcg gtcgatcatg tcgcccggca
      901 tgtgcgggta gcggccgtcc atgttccgcc gcatgttctc gtgggcgacg aacctcgcgg
      961 tgtcggcgaa gacggctccg ccggccgcat gatcgaaatg actgtggctg tagaccacgt
     1021 accgcaccgg gaccgtaaag cggcggccga gctcgtcctt cagccatgac gcgaacgccg
     1081 ggttgatcgg gtcgccgagg ataatgccgt cgcgggtgac gagaaaaatc gcgtaccagt
     1141 tgccgttgcg ggcccgatac aggtcgccgg tgagcggcac gatctcgcgt gccggccctc
     1201 gcggctgcgc ctgcacgacg gacagcgcgg caacgagcgt cgcgagcagg gcagcgaggc
     1261 cggtgcgtgt gcgggacatc acgggcgcgt gcccgcgatg atatagaagc gcttggacgg
     1321 gggcaaaggc caatggtcgg cagcacacat ataatccaac cgccttcctg gagacagcat
     1381 tggggacgac cagcgaaaca ccggtcctca tcgtcggcgg cggcgccgca ggctcggtgc
     1441 tcgccatgga gcttgcgcgg cgcggggtcg agttccgctg catcgatcgg atgccgggac
     1501 cgggacgtga atcgcgcgcc atcgcgcttc acgcccgcac gatcgagttg atggacctgg
     1561 tcgacacggc cctgagccgg cgcatgctcg atcgcgacct gtggtgcaag ggctacgtga
     1621 tgcacttcct ccgcgggcgc gagcgcaccg aggtgcgccc gggcctcgac tacacgaagg
     1681 tggactccag atacaactgc atcctgtcgc acaaccagag cgagaccgag ggcttcattc
     1741 gcgagtacac aaggtcgcgg ttcggcaggg aagtcgaata cgacacggcg tttcaggatc
     1801 tgacccagga cggcgacctc gtgacggccc gcgtcacgca cggcgatcgc ggcgaccagg
     1861 aggagttcat ccgctgccgg tacctcatcg cggccgacgg catcaattcg cgcgtgcgcc
     1921 gcggtctcgg gctgtcggtc aaggggaagg actacaaggg cagcttcttc cagaacctgg
     1981 acatccacct gaagggcttc accgactggc gggactactt ccactactgc gtcggcaccg
     2041 accacttcct catggtggcg ccgctgccgg gcggacactt ccggctgctc ctgagcgatc
     2101 gcggcgaagc cgccgacccg acgctcacgc cgcagcaggc cttcatgcgc ctgctcgatc
     2161 agcactacga cggcatcacc atgggcgagg tcgtgtggca ttcgaaatgg gagacctggg
     2221 ttcgtctcgc cgacaccttc cgtcagggca acgtgttcct ggccggcgat tcggcccacg
     2281 tccactcgac gacgggcggt caggggatga actgctgcat gcaggacgcg ttcaacctgg
     2341 gatggaagct cggcctggtc ctgaaggggt acgcccggcc cacgctgctc gacacgtacg
     2401 aggccgaacg gcggcctgtc gccgagcagg tcatctgggc agcttcatcg ctgcacgaca
     2461 tcttcatgac ccatggtaag gacatcgcgc agcgcacgca gacgatgttc gagcctggct
     2521 atacagagaa ggtggtgaac tcctgctcgg gtgtcgccta cacgtacaag gacttcatcg
     2581 acaagccccc ggggttgcgc gagattgacg gccctgccat cggcgaccgg gcgcccgacg
     2641 tcgacttcga gcgtggcggt accctcttcg accggctccg gcatacgtat ttcacgctgc
     2701 tggcgatgcc cgatggcgat ctgagtccga tcgtggagcg gctcggccgt cgctttggcc
     2761 ccgtgctgga cgtggagatc cttccgaaat cggacgcgct ctcggcgcgt tacgggcaga
     2821 gcgacggccg gctgtttctc gtgcgcccgg atggctacat cgccttcaag agctgcgcgg
     2881 acgaggccgg ccttct
//
LOCUS       DBNGSH010000105         2247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3912652, whole genome shotgun sequence.
ACCESSION   DBNGSH010000105 DBNGSH010000000
VERSION     DBNGSH010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3912652"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1224)
                     /locus_tag="AC16CY_02315"
     CDS             complement(247..1224)
                     /locus_tag="AC16CY_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352639.1"
                     /translation="MRSVLTITALVYIVSQMAPAGAQTRREPVWNERTAWGDPDLQAE
                     WTSEGEYGVPLERPPQFGARQFLTDEEYARRLEEVRLRDERDLAPVDVLSGKVDAPNA
                     PIPHWREYNTTSRRTSLIVDPPDGRLPPRTPQAKPIPVQRCGSLQRGEPCDSYIDYGL
                     GVRCIVHGGGFPDAMFPAVYNANMRILQTPEYVAIVYELIHDARIIPLKRDGPPLSPA
                     IRTYMGDARGRWEGTTLVIESGNFKANTRGASPGLRLIERFTRVGRDAVQYQVTFVDP
                     ATWTAPWAAALDLRRRPEGAGVFEYACHEGNYGLFNMLSASRHLERTAR"
     gene            complement(1221..2069)
                     /locus_tag="AC16CY_02320"
     CDS             complement(1221..2069)
                     /locus_tag="AC16CY_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3352640.1"
                     /translation="MKGAILVGSIAAVLTVGLTAQTPARALPLENLGLEHLDIVVPDP
                     AASARFYARLFRSALHQQPVRDTLRYFVLLGDLPADRQIGYIAIGAAGGRAPAVGHYC
                     VLAKVYDRAGVASTLQAAGFAVASAGPTGMWPDPDRLELQLFQPPAGLVAAAVPSPLP
                     VERDGLLTPRGVDHVMLRVSSLEKSLPYYRLVYGAAAEQPRDTNGRVWFHLARNTRLG
                     LEEASSGQPPAIAHYTIKVAPYDRGAFESRLRELGARVLQAADEPGVVRFADNDGVIV
                     EVRVAE"
BASE COUNT          388 a          794 c          718 g          347 t
ORIGIN      
        1 cccgactaca tgaagggcca gtacagcggg tcggacacga tcggggttgc agaccagccg
       61 tacgccgaag acaactgcaa ggagcagacg ctggtcgatt actcgaagca acagtcccag
      121 cgataaaagc gacggcgggt ctgccttcga cttcgctcag tccaggaacc ccccactacc
      181 aaagagccgc taccgtagag gcgggtcttc agacccgcct accgatgcac ccggcaagca
      241 cagtcgtcat cgtgccgttc gctcgaggtg ccgcgacgcc gacagcatgt tgaacaggcc
      301 gtagtttccc tcgtggcacg cgtactcgaa cacgccggcg ccctccggcc gcctcctgag
      361 gtcgagcgcg gcggcccagg gtgcggtcca cgtcgcggga tcgacgaagg tgacctggta
      421 ctggaccgcg tcccgcccca cgcgggtgaa ccgctcgatc agccggagcc cgggagatgc
      481 cccgcgcgtg ttcgctttga aattgcccga ctcgatgacc agcgtcgtgc cctcccaccg
      541 gcctcgcgcg tcgcccatgt acgtccggat cgccggcgac aacggcggcc cgtcgcgctt
      601 cagcggaatg atccgcgcgt cgtgaatcag ctcgtacacg atcgcgacgt actccggagt
      661 ctgaaggatc cgcatgttcg cgttgtagac cgcgggaaac atcgcatccg gaaaaccgcc
      721 gccgtgcacg atgcagcgca cgccgagccc gtagtctatg tacgaatcgc acggctcccc
      781 ccgctgcagg ctgccgcatc gctgcactgg aatcggcttt gcctgcgggg ttcgcggagg
      841 cagacgccca tcgggcggat cgacgatgag cgacgtccgc ctcgatgtcg tgttgtattc
      901 gcgccaatgc gggatcgggg cattgggcgc gtccactttg ccggagagga catcgaccgg
      961 cgccagatct cgctcgtcgc gaagccgcac ctcttcgagc cgccgggcat actcctcgtc
     1021 cgtcaggaac tgtcgcgccc cgaactgcgg cgggcgctcc agcggcacgc cgtactcgcc
     1081 ttcgctcgtc cactccgcct gcaggtcggg atcgccccag gccgtgcgct cgttccagac
     1141 cggttcacgc ctcgtctgtg cgccggccgg ggccatctgc gagacgatgt acaccaaagc
     1201 cgtgatcgtc agcacgcttc tcattccgcg accctcactt ccacgatgac gccgtcgttg
     1261 tccgcgaaac ggacgacgcc gggctcgtcg gcagcctgaa gcacacgcgc gcccagctcc
     1321 cgcagccgtg actcgaacgc gccgcgatcg tacggcgcga ccttgatggt gtaatgggcg
     1381 atcgcgggcg gctgccctga cgatgcctcc tccaggccga gacgggtatt gcgcgcgagg
     1441 tgaaaccata cgcgcccgtt cgtgtcgcgg ggctgttcgg ccgctgcgcc gtacaccagc
     1501 cggtagtacg gcagcgattt ctcgaggctg ctgacgcgca gcattacatg atcgacgccg
     1561 cgaggcgtga gcagcccgtc gcgctcgacc ggcagcggcg acgggacagc ggcggccacg
     1621 agaccggccg gcggctggaa cagctgcagc tcgagccggt cgggatccgg ccacatcccc
     1681 gtcggccccg ccgacgcaac ggcgaagccc gcagcctgca gcgtgctggc cacgcccgcg
     1741 cggtcgtaga ctttggccag cacgcagtaa tggccgaccg ccggcgcgcg cccgcccgcg
     1801 gcgccgatgg cgatgtagcc gatctgccga tccgccggaa ggtcgccgag caacacgaag
     1861 tagcgcagcg tgtcgcgcac cggctgctga tgcagagccg atcggaagag acgggcgtag
     1921 aaccgcgcag atgccgccgg gtccggcacg acgatgtcga gatgctccag gcccaggttc
     1981 tcgagcggca gcgccctcgc cggtgtttgc gcagtcaggc caaccgtgag cacggcggca
     2041 atcgatccca cgagaatggc gcccttcatc tacaccacag ctcgcttgtg ttggtgggct
     2101 ccggccgagg ccccgctgaa caaacagcct gccctgcaag ccacgatcgt tggcaacatc
     2161 accgacacaa gcgagttccg gggttaagtc tccgtctgcg atccctcacg tcctgccacc
     2221 tcaatgcgtg gagctctgca cgagata
//
LOCUS       DBNGSH010000106         1858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4252131, whole genome shotgun sequence.
ACCESSION   DBNGSH010000106 DBNGSH010000000
VERSION     DBNGSH010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4252131"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1252
                     /gene="hemC"
                     /locus_tag="AC16CY_02325"
     CDS             263..1252
                     /gene="hemC"
                     /locus_tag="AC16CY_02325"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR3352641.1"
                     /translation="MPALRLGTRGSPLALYQANAVAGALQARAGVPCEIVVIRTSGDQ
                     LAEASAPPDASALADAAGGKRLFVKEIEDALLAGSIDLAVHSSKDMPVLLPEGLRIGA
                     VLPREDPRDALVLPEGRSAGLGLDEVLRLLGAAPVIGTTSVRRIAQLARVSPGARFAP
                     ARGNVGTRLRKLDAGDYDALVLAAAGLARLGLGARVSALLPADVSVPAPGQGIIAVEV
                     RHDDRREELNVIDDADASLALRAERAVVRRLGGGCQMPIGAYASIAGGRLSITGVVIS
                     PDGLRMARADASGPAVAPEEVGGRAADELLAQGGDKILADVRRALSPAERHHT"
     gene            1249..>1858
                     /locus_tag="AC16CY_02330"
     CDS             1249..>1858
                     /locus_tag="AC16CY_02330"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF012799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3352642.1"
                     /translation="MKNPAVYVIGTGPGDPGLITAHGLECLSRADVVIHDHEVPVRLL
                     KQAREGAELIDVGSAVRQPVAQDAITFLLAEKARDGKLVARLALGDPFVFDRGGEEAL
                     FLHEQGIPFEVVPGIPLGIGVPAYAGVPVTYPGGGDTITLVRGHEDENRAPLPDVDWA
                     SLAKLDGTVVCYAGSQQLPRILQALVSNGWPEDGHGVIVYNGT"
BASE COUNT          280 a          633 c          684 g          261 t
ORIGIN      
        1 gaggctatca accgcctctt ccgcctgcgg gaaggcgagc ccgtgccggc cccggacgaa
       61 ggcgccagcg aggtgacgcc ggccgatcgt cgaggcaagt aatggacggt atcaactgga
      121 agagtaccgc gaacaacgag cgtcgacggc gatttcccgg ccgcgacaac cggggtcccc
      181 gccgcgcgtg gtgtgcgcgg tggggtggga gcggagcggc cggtggtcat ccgcgaagga
      241 cataccacta gtcggcccga agatgcctgc gctgagactc ggcacgcgtg gcagcccgct
      301 cgcgttgtat caggcgaatg cggtcgccgg cgcgcttcag gcgcgcgccg gcgtgccgtg
      361 cgagatcgtc gtcattcgca cgtctggaga ccagctggcg gaagcctcgg ccccgccgga
      421 cgcttctgcc ctggccgacg cggcaggcgg caagcgtctg ttcgtgaagg aaatcgaaga
      481 cgcgctgctc gcggggagca tcgacctggc cgtgcacagc agcaaggaca tgccggtgct
      541 gctgccggaa ggtctccgca tcggcgccgt gctcccgcgc gaggatccgc gtgacgcgct
      601 cgttctgccc gagggacgga gcgccggcct cggcctggac gaggtactgc ggctgctcgg
      661 agcggcgccc gtcatcggca cgaccagcgt gcggcgcatc gcacagctcg cacgcgtcag
      721 ccccggcgcc cggttcgcgc ctgcgcgggg caacgtcggc actcgcctgc gcaagctcga
      781 cgcgggcgat tacgacgcgc tcgtcctcgc cgcggccggg ctcgcgcggc tggggctcgg
      841 agcgcgcgtc tcggcgctgc tgccggccga cgtctcggtg ccggcgcccg gacagggcat
      901 catcgctgtc gaggtgcggc acgacgaccg gcgcgaggaa ctgaacgtga tcgacgacgc
      961 cgatgcgtcg ctggctctgc gggccgagcg agccgtcgtc cggcgcctgg gcggcggctg
     1021 ccagatgccg atcggggcct acgccagcat tgcgggcggc cggttgtcga ttaccggggt
     1081 ggtcatctcg ccagacggcc tgcggatggc gcgggccgac gcctccgggc cggccgttgc
     1141 accggaagag gtcgggggac gagccgcaga cgaactgctg gcacaaggtg gagataagat
     1201 cctggccgac gtcaggcggg cactgtcgcc ggccgaacgt catcacacat gaagaacccg
     1261 gcggtttacg tcatcgggac agggcccggc gatcccggcc tcatcacggc gcacgggctg
     1321 gaatgcctct cgcgggccga cgtcgtcatc cacgaccacg aggtcccggt gcggctgctg
     1381 aagcaggcgc gcgagggtgc cgaactgatc gacgtcggca gcgccgtgag gcagccggtg
     1441 gcgcaggacg cgatcacgtt cctgctcgcc gagaaggcgc gtgacggcaa gctcgtcgca
     1501 cgattggcgc tcggcgaccc cttcgtgttc gatcgcggcg gcgaggaagc gctgttcctc
     1561 cacgagcagg gcatcccctt cgaggtcgtg cccgggattc cgctcgggat cggcgtgccg
     1621 gcatatgccg gagtccccgt gacctatccg ggcggcggcg acaccatcac gctcgttcga
     1681 ggccacgagg acgaaaaccg cgcgccgctg cccgacgtgg actgggccag tcttgcgaaa
     1741 ctcgacggga cggtcgtctg ttacgccggc tcacagcagc ttccgcgcat cctgcaggcg
     1801 ctcgtgtcca acggctggcc cgaagacggg cacggcgtca tcgtctacaa cggcacgc
//
LOCUS       DBNGSH010000107         1881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_177177, whole genome shotgun sequence.
ACCESSION   DBNGSH010000107 DBNGSH010000000
VERSION     DBNGSH010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_177177"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..>1881)
                     /locus_tag="AC16CY_02335"
     CDS             complement(299..>1881)
                     /locus_tag="AC16CY_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352643.1"
                     /translation="AGSYVLAYLTMAGGAAALYLLLRDTLHDRTAASVVGVAWAGCTW
                     AHGIGGWNYHMLAASAYYLAACWLVVCAARCHSAYVVSGFSRTSMSATMAGVFLASSV
                     HAYIVMVTFAPLVAFLYWAALPRGLERPFARSARTALWVLGGGLLITLSLAIVNRATG
                     GAWLFFMPQIEAAVKYSQPDADIWWMANVKDWLPSALYLVIPMSSLIAGLAVAFGRRD
                     DPDRRMKLTLVVLAWAGVAIMCWFQFVRHQVALDYSYHAFPIYLYAFPCLAAALSDRL
                     RESRPILVAAAFLAVVGTLLLFLPAPLPRAMDAMSAAVGLDRFPAVVSPAVFALAGVT
                     AMMLMRDRLRILMFVVWFSILNAWIAPERSSYGVNTPGYRAQMIELFVEADRFTAGLD
                     PSLIGIKYWLSNEQVTTAHGTVPLRPVFDSFVATRAWFTNLLGRVAPSPPVEQLTLDD
                     LERGVCIGVLSSLEAQDGLVKGIETRYAELGRPLRVVAARRFERRDLSFALTVLKPSP
                     GEGRESATTTPCMRADVK"
BASE COUNT          377 a          631 c          617 g          256 t
ORIGIN      
        1 cttcacggta gcgctgccaa cggtgttgtc ccactcggtg agcatctgcc cgttcaggcc
       61 gctgacgtag tagaacgtcc gccccgccgt caaaccgcct tcttcaccgc ccgcctggcg
      121 cattagatgc cagatcttca tcggcaggtg cccgcggcac gattgcggca gctggtcgga
      181 gttgtcaaga cggcgaccgc agaaaccatg caaattgact tgcgcgggtg ttgctcggtc
      241 cggctgaagc cggacactac gtacgaccgg cgttcacggg tccggctgaa gccggacact
      301 acttgacgtc cgcgcgcatg cacggagtag tcgtcgctga ctctcttccc tcaccgggcg
      361 acggtttgag cacggtgagc gcgaacgaga gatcgcggcg ctcgaagcga cgggccgcca
      421 cgacgcggag tggccggccc agttccgcgt agcgcgtctc gatgcccttc acgagcccgt
      481 cctgggcctc cagcgacgac agcacgccaa tgcagacgcc gcgctcgagg tcgtcgagcg
      541 tcaactgctc caccggcggg ctcggggcga cgcgccccag caggttggtg aaccacgccc
      601 ttgtcgccac gaagctgtcg aaaacgggtc ggagcggcac ggtcccgtgc gccgtcgtga
      661 cctgttcgtt cgagagccag tatttgatgc cgatgagcga ggggtcgagc ccggccgtga
      721 accggtcggc ttcgacgaac agctcgatca tctgggcgcg gtacccgggc gtgttcacgc
      781 cgtacgacga acgctcaggc gcgatccatg cgttgaggat cgagaaccac acgacgaaca
      841 tcaggatccg gaggcgatcc cgcatcagca tcatggcggt cacgccggcg agggcgaaca
      901 cggccgggga gacgactgcc ggaaatcgat ccaggcccac ggcggcggac atcgcgtcca
      961 tcgcgcgcgg caacggcgcc ggcagaaaga gcagcagcgt gcccacgacg gcaagaaaag
     1021 ccgccgccac gagaatcggc cggctctccc ggaggcgatc cgacagcgcc gccgccaggc
     1081 agggaaacgc gtacaggtag atcggaaacg cgtgatagga gtaatcgagc gcgacctggt
     1141 ggcggacgaa ctggaaccag cacatgatgg cgacgcccgc ccacgccaga acgaccagcg
     1201 tcaacttcat ccggcgatcc ggatcgtccc ggcgcccgaa cgccaccgca agcccggcga
     1261 tcagagaaga catcgggatc accaggtaga gcgccgacgg gagccagtcc ttcacattcg
     1321 ccatccacca gatgtccgca tccggctgtg agtacttcac ggccgcttcg atctgcggca
     1381 tgaagaacag ccaggcgcct cccgtggcgc gattgacgat ggcaagggac agcgtgatca
     1441 agaggccgcc gcccagcacc cacaacgccg tccgcgcgct tcgtgcgaac ggccgctcga
     1501 ggccccgggg gagcgcggcc cagtagagaa acgcgacgag cggcgcgaag gtcaccatga
     1561 cgatgtacgc gtggacggac gaggcaagaa agactccggc catcgttgcc gacatggagg
     1621 tccggctaaa gccggacact acgtacgcag agtggcaacg tgcggcgcag acaaccagcc
     1681 agcacgcggc gaggtaatac gccgacgcgg caagcatgtg gtaattccat ccgccgattc
     1741 cgtgcgccca cgtacacccc gcccaggcga cgccgaccac cgaggcagcg gtccggtcgt
     1801 gcagcgtgtc gcgcagcagc aggtagagtg ccgccgctcc tcccgccatc gtgaggtagg
     1861 cgagcacgta cgatcctgcg a
//
LOCUS       DBNGSH010000108         3522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3742850, whole genome shotgun sequence.
ACCESSION   DBNGSH010000108 DBNGSH010000000
VERSION     DBNGSH010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3742850"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="AC16CY_02340"
     CDS             complement(<1..221)
                     /locus_tag="AC16CY_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR3352644.1"
                     /translation="MTTTFNRRHFLHVSAAAGGGLLVGAYLPWKGSVADAAGVFEPNI
                     WIKINADDTVRIMLTMLEMGQGVMTSMPML"
     gene            complement(288..737)
                     /locus_tag="AC16CY_02345"
     CDS             complement(288..737)
                     /locus_tag="AC16CY_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3352645.1"
                     /translation="MIALEVNGKRVNVDVSPDMPLLWVLRDTLGLTGTKFGCGLGQCG
                     ACTVHLDGAAVRACTTAVSAASGKKITTIEGLSPNSSHPLQVAWIAEQVPQCGYCQSG
                     QIMSAAAMLAKTPRPTDAQIDSAMAGNICRCGTYQRIRRAIRRAARV"
     gene            807..1589
                     /locus_tag="AC16CY_02350"
     CDS             807..1589
                     /locus_tag="AC16CY_02350"
                     /inference="COORDINATES: protein motif:HMM:NF039623.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HIR3352646.1"
                     /translation="MAYQTFDDVISGLAAIEARFTQRGDRRAIFATLYGVISTAAREN
                     VGQHLFEDNAWVGRYAVTFADFYRVALERYEAGQMSDVPKAWRLCFDFAREGDGLVLQ
                     DLLLGVNAHVNNDLPLALNAVSIDPDRASRHRDHTAVNRVFGRALERATEAVATLYAP
                     GLETADDFGGQLDELASLFSIEVARESAWEAAVALANARSDAERRLTTTLVSARAAVV
                     ARLLRAPSLSPTFIAACRRLEQQPNWVALASECIRLTRTASG"
     gene            complement(1567..2889)
                     /locus_tag="AC16CY_02355"
     CDS             complement(1567..2889)
                     /locus_tag="AC16CY_02355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIR3352647.1"
                     /translation="MAFDEIFQPLRFRNLTIKNRILRSNISGRFDNYDGSGNQARINW
                     EVKFAKGGVGAIVSSFVPVHLRGRIVPNYAMIDHDRHIPFWRAVGRAVHEHDCKFILQ
                     LSHSGRQRDIPGIEYATALSSTPKPDPTHGFRCEQATIPQITEIVNAFAEGARRAREA
                     GLDGVELHGANGYLITQFLSSAINDRKDEYGGSLENRARFVLEVVRAIRRRVGDDFHL
                     QMKISVHEYNDAIAYFGLGPSGNRPEESVQVCKWLEEAGVDAIHVSIGSFFPHPRNPA
                     GVDLPAEDLAKTYDNLISSGDRAFRNYLLFRSLPALARRQWNEAAPQPQNIEGANLPD
                     ARRVKAAVSIPVICTGGFQTASYIAGALRRGDCDAVSIARPLIANNDLVRVFERGQDR
                     AERPCTYCNKCLANVVENPLGCYELSRFPSQEAMVAEIMSVFNPTPFA"
     gene            3216..>3522
                     /locus_tag="AC16CY_02360"
     CDS             3216..>3522
                     /locus_tag="AC16CY_02360"
                     /inference="COORDINATES: protein motif:HMM:NF025589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HIR3352648.1"
                     /translation="MAVDASTPVSVIDGIRPAIEAGLARLGVFAVFDLLRGTSEHLHS
                     VVKANASLADVESWKCQAALLQVQAVTPAWAAALVRAKVMTVDGLALKSVSAIAQILA
                     "
BASE COUNT          562 a         1152 c         1201 g          607 t
ORIGIN      
        1 agcatcggca tcgacgtcat cacgccctgc cccatctcga gcatcgtcag catgatgcgc
       61 accgtgtcgt cggcgttgat cttgatccag atgttgggct cgaacacgcc cgccgcgtcg
      121 gcgacgctgc ccttccacgg cagatacgcg ccgacgagga gtccgccacc ggcggcggcg
      181 ctgacgtgca ggaagtgccg gcgattgaaa gtcgtcgtca tgatggcctc attcgaacct
      241 gtgcccccgt gcaccttcgc accttcgcac cctcgcacct tcgcacccta taccctcgcc
      301 gctcgccgaa tcgcgcggcg gatgcgctgg taggtgccgc accggcagat attgccggcc
      361 atcgccgagt cgatctgggc gtcggttgga cgcggtgtct tcgccagcat cgcggcggcc
      421 gacatgatct ggccggactg gcagtacccg cactgcggga cctgttccgc gatccacgcg
      481 acctgcagcg ggtgcgacga gttcggcgac agcccctcga tcgtcgtgat cttcttgccg
      541 gatgcggccg agacggccgt cgtgcacgcg cgaaccgccg ccccgtcgag gtggacggtg
      601 caggcaccgc actggccgag gccgcagccg aacttcgtgc cggtgagacc gagcgtgtcg
      661 cgaagcaccc acagcagcgg catgtcgggg ctgacgtcga cgttcacgcg tttgccgttc
      721 acctcgagcg caatcatgga agctcctccg tcgccccggc gttacgaaaa cgtaatgggg
      781 tctgacccca attatgatcc aggtccgtgg cgtaccaaac gttcgatgac gtcatttccg
      841 gtctggctgc catcgaagcg cgcttcacgc agcgaggcga caggcgggcc atcttcgcca
      901 cgctctatgg cgtcatatcg accgccgcgc gcgagaacgt cgggcagcat ctcttcgagg
      961 acaacgcgtg ggtcgggcgg tacgccgtga cgttcgccga tttctaccgg gtggcgctcg
     1021 agcgctacga agccgggcag atgagcgacg tgccgaaggc gtggcggctg tgcttcgact
     1081 tcgcgcgcga gggcgatggc ctcgtgctcc aggacctgct gctcggcgtg aacgcccacg
     1141 tcaacaatga cctgccgctg gcgctgaacg ccgtctccat cgacccggac cgggcatcgc
     1201 ggcaccgtga ccataccgcc gtgaacaggg tgttcggccg cgccctcgag cgcgcgacgg
     1261 aggctgtcgc cacgctgtat gcgcccgggc tcgagaccgc cgacgacttc ggcggtcagc
     1321 tcgacgagct cgccagcctg ttctcgatcg aagtggcgcg cgagagcgcg tgggaggcgg
     1381 ccgtcgcgct cgccaatgca cggtccgatg ccgagcggcg gctcaccacg acgctcgtat
     1441 cggcgcgcgc ggcggtcgtg gcaaggcttc ttcgcgcgcc gtcgctcagc ccgacgttca
     1501 tcgccgcgtg ccgccggctc gagcagcagc cgaactgggt tgcgctcgcg tcggagtgca
     1561 ttcgccttac gcgaacggcg tcgggttgaa gacggacatg atctcggcca ccatcgcctc
     1621 ctgcgaggga aagcgcgaca gctcgtagca gccgagcggg ttctcgacga cgttggccag
     1681 gcacttgttg cagtaggtgc agggccgctc ggcgcggtcc tggccccgct cgaagacgcg
     1741 gacgagatcg ttgttggcga tgagggggcg cgcgatgctg acggcgtcgc agtcgccgcg
     1801 ccgcagggcg ccggcaatgt aggacgcggt ctgaaagccg cccgtgcaga tcaccgggat
     1861 cgacaccgcg gccttgacgc ggcgggcgtc cggcaggttc gccccctcga tgttctgcgg
     1921 ctgcggcgcc gcctcgttcc actgccgccg cgcgagcgcc ggcaggctcc tgaagagcag
     1981 gtagttgcgg aaggcgcgat ctccggacga gatcaggttg tcgtaggtct tggccaggtc
     2041 ctcggccggc agatcgacgc ccgccggatt gcgaggatgc gggaagaagc tgccgatcga
     2101 cacgtggatc gcgtccacgc ctgcctcttc gagccacttg cagacctgca ccgactcctc
     2161 ggggcggttg cccgacggac ccagaccgaa gtaggcgatc gcatcgttgt actcgtgcac
     2221 gctgatcttc atctgcaggt gaaagtcgtc gcccacgcgg cggcggatcg cccgcacgac
     2281 ctcgagcacg aagcgcgcgc ggttctcgag cgacccgccg tactcgtcct tccggtcgtt
     2341 gatcgccgag ctcagaaact gcgtgatgag gtacccgttc gcgccgtgca actcgacgcc
     2401 gtcgagcccg gcctcgcgcg cgcggcgcgc tccttcggcg aacgcgttga cgatctcggt
     2461 gatctgcggg atggtcgcct gctcgcagcg gaagccgtgc gtggggtcgg gcttgggcgt
     2521 ggagctgagg gcggtcgcgt actcgatgcc cggaatgtcc cgctgccggc cgctgtggct
     2581 gagctgcagg atgaacttgc agtcgtgctc gtgcacggcg cgccccacgg cgcgccagaa
     2641 cgggatgtgg cgatcgtggt cgatcatcgc gtagttcggc acgatgcgcc cgcggaggtg
     2701 cacggggacg aacgacgaga cgattgcgcc gacgccgcct ttcgcgaact tgacctccca
     2761 gttgattcgc gcctggttgc cggagccgtc gtagttgtcg aagcggcccg agatgttgga
     2821 tcggagaatg cggttcttga tcgtcaggtt gcggaagcgc aacggctgga agatctcgtc
     2881 gaacgccacg gcttgacctc cggtggatgg ggccgacgcc aactccgcag acgttcggaa
     2941 tcagctggtc acgtgagagg gagtcgaagg ttagcgcgcg cccacgagag agtcaacgac
     3001 gcgtgcacgg atttcgaaca tcgcgtgcgg ctgagctcga tcggacggcc cgatgagctt
     3061 cgacttaatc aaggcacccg tgaagagcaa cgggagctca tagagcggct gctctcgcga
     3121 ctcggaacga gcggtcagct cgaatcgatt cgccgtgttt gtttcacgat cgcgttcgga
     3181 taccatgagg cccccaaatt cattcaggga gcgccatggc tgtcgacgca agcacgccag
     3241 tttccgtcat cgacgggatc agaccagcga tcgaagcagg actggctcgt ctcggtgtgt
     3301 tcgccgtctt cgatcttctg cgaggaacgt cggagcacct gcactcggtc gtcaaggcga
     3361 atgcgtcgct ggcggatgtc gaatcctgga aatgccaggc cgcgctgctg caggtccagg
     3421 ccgtcacgcc cgcctgggcg gcggcgctcg ttcgcgccaa ggtgatgacg gtcgacggtc
     3481 tggcgctgaa gagcgtgagc gccatcgccc agattctggc gg
//
LOCUS       DBNGSH010000109         5025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1365495, whole genome shotgun sequence.
ACCESSION   DBNGSH010000109 DBNGSH010000000
VERSION     DBNGSH010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1365495"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="AC16CY_02365"
     CDS             <1..677
                     /locus_tag="AC16CY_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352649.1"
                     /translation="STRLRAVRGSTFHRSPVMSARVVLAVAFSTVVATPCAQAAETVW
                     TDIAIRVYDTSGISAGDRQASLKIAASIVSAASLEAIWRICDEKGAGGRGQGAGQCDG
                     PMRAGELSLRIVRSVVPSGYAGKLPLGDALIDPRSGAGTLATIYIDRVEWMAKQTGTD
                     PRALLGRAIAHELGHLLMPASGHSTRGLMRAIWSGAELRRGRARDWAFEPREVAAIRA
                     RFTEPR"
     gene            complement(929..1993)
                     /locus_tag="AC16CY_02370"
     CDS             complement(929..1993)
                     /locus_tag="AC16CY_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3352650.1"
                     /translation="MDDLPAIGAPPPRPRLPKIAVEEHFNYLTAGPGDRAHVDITSLV
                     KSMDYDQAWSALVGERLSDVGERRIAAMDAGGIDVCILSHTVPGVQGIVDAADAVAAA
                     RDVNDFLAGRIASHPTRFAGFASVPLQDPAAAAQELDRCVSRLGLKGAMVNGYTDVAD
                     GRGGAYLDEPAFLPFWEAVSSLAVPVYLHPRPALEQRIYQGHSQLIGATWGFAPETAT
                     HALRLVHGGLLDRFPDLTIVLGHLGETLPYFAWRIQHCFEYNPSDKRLERRLQDYLCE
                     NFFVSTSGIFSDQALIGALLTIGADRILFAVDYPYERMGPASDWIDRAPISENDRRKI
                     AHLNARTLFRLEERLISEGT"
     gene            complement(1999..3030)
                     /locus_tag="AC16CY_02375"
     CDS             complement(1999..3030)
                     /locus_tag="AC16CY_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352651.1"
                     /translation="MTRHWLAVAGVAVGVVAGTSASLDSQQTGRSAVSAPAATAKPYV
                     APRTPWGDPDLQGGWTNVSENGIPFERPDALAGKQLEQVDDSELAELDKARDEARLRG
                     AAAIGGRDTGAGPVHWYEHYGAKNSRAWMVIDPPDGRIPAQTAAAQARIAGRIGGRGG
                     RGGGFAEGGRADSWLDRSLYDRCISRGIPGSMMPAIYGNAYDITQAPGIVAIRYEMIH
                     ETRIIRLDNSPRSGLPSHMGDARGRWEGDTLVVETVGFDPRSVYRNGSAKMKLTERFK
                     PVAKNKIDWSMTVDDPETWTRPWTFGMTLTADPEQPLFEYACHEGNYAMRNILSAARA
                     EEARPGATR"
     gene            complement(3095..3805)
                     /locus_tag="AC16CY_02380"
     CDS             complement(3095..3805)
                     /locus_tag="AC16CY_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3352652.1"
                     /translation="MTSPSDIDLLHEIGSRLAAADPLHDVLSRVVDVVSALVHCDSCF
                     VYVLQDEDLVLRASKTPHPDVVDRLTLRLGQGITGWVAAHRQPVAVGRHAFEDPRFQT
                     FNELPEDRYEAFLSVPVLSRGKPVGVINLQHRQPHTHTQREIQLISTIGFLVGAEIET
                     ARLEMENSQLFERLETRKLLDRAKGILQRDLGLTEEDAYLTIQRQSRQRRKSKKEIAE
                     AIILGDELRRGKAGPDQR"
     gene            complement(3866..4225)
                     /gene="nirD"
                     /locus_tag="AC16CY_02385"
     CDS             complement(3866..4225)
                     /gene="nirD"
                     /locus_tag="AC16CY_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HIR3352653.1"
                     /translation="MTAHRWIRVTSIESIPEREGRAVQIGNREIAIFNLGDRFLATDN
                     RCPHAGGPLCDGIVTGASVVCPLHSWKVSLASGSVERPAGSERCVTTYPARVEDGLVM
                     VALPDPSNMLHESASHT"
     gene            complement(4222..>5025)
                     /locus_tag="AC16CY_02390"
     CDS             complement(4222..>5025)
                     /locus_tag="AC16CY_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HIR3352654.1"
                     /translation="SARFINDRVHANIQKDGTFSVVPRIRGGVTNPAELRRIADVAEK
                     YQVPMVKITGSQRIDLLGIRKADLPKVWADLGMPSGQAYTKGVRMVKTCVGTEFCRFG
                     TQHSTAAGIEMERRFEQLYTPHKVKMGAVGCPRNCAEATVKDIGLVGIEGGWQVVVGG
                     AAGKSVRKADLLTTVETTEEALEAAELFFQYYRENANYLERTYDFVERLGIEKVRKDT
                     VYATDATRSALLDRLRKSKARARDAWHEGQAPRHPTQFEPISVVDKVLA"
BASE COUNT          784 a         1703 c         1670 g          868 t
ORIGIN      
        1 aatcgactcg tctccgcgcg gtgcgcggat cgaccttcca caggagcccg gtcatgtccg
       61 ctcgcgttgt cctcgcggtg gctttctcga cggtagtggc gacgccgtgc gcgcaagcgg
      121 cggaaactgt ctggaccgat atcgccattc gtgtctacga cacgagcgga atttccgccg
      181 gcgaccgtca ggcgtctctc aagatcgcag cgtccatcgt gtcagcggcg tcgctcgagg
      241 cgatctggag gatctgtgac gagaaggggg cagggggcag ggggcagggg gcagggcagt
      301 gcgacgggcc catgcgggct ggagagctct ctctccggat cgtgcgctcc gtcgtgccgt
      361 ccggctatgc gggcaagctg ccgctcggcg acgcgctcat cgatccgcgg agtggcgcgg
      421 gcacgctggc cacgatctac atcgaccgcg tggagtggat ggcgaagcag acgggcacgg
      481 atccgcgcgc gctgcttgga cgcgccatcg cacacgagct cggccatctg ctgatgcccg
      541 cgagcggcca cagcacgcgc ggcctgatgc gcgccatctg gtcgggagcg gagctgcggc
      601 gcgggcgcgc ccgcgactgg gcgttcgagc cgcgcgaggt ggcggcgatc agggcgcggt
      661 tcaccgagcc gcgctgacgc tgactacgtg aggtcacttc cattcagcag aggatctgtc
      721 gttcacgctt ctccgttctc ggttctcggt tcgtgttcaa tttcaggttc gtgttctgag
      781 ttcactgttc ggcgttcgcg gttcgggacc acgtctcggc accccgaacc cagcacctcg
      841 caccgaaaac ctcgaacgcg aacctgaaac tgaacacgaa ctgagaagtc agaagcgaga
      901 agtgtgaacg acagatctcg tgctcaattc atgtgccctc ggagatcagt cgctcttcca
      961 gcctgaacaa cgtccgcgcg ttgaggtgcg cgatctttcg gcggtcgttc tcgctgatgg
     1021 gcgcgcggtc gatccagtcc gacgccggcc ccatccgctc gtaggggtag tcaaccgcaa
     1081 acaggatgcg atcggcgccg atggtcagca gcgcgccgat cagcgcctgg tcgctgaaga
     1141 tgccgctcgt cgagacgaag aagttctcgc agagatagtc ctgcagccgc cgctcgagcc
     1201 gcttgtccga cgggttgtac tcgaagcagt gctggatgcg ccacgcgaag tagggcagcg
     1261 tttcgccgag gtggccgagc acgatcgtca aatccggaaa gcggtcgagc aggccgccgt
     1321 gcacgagtcg cagcgcgtgc gtggccgtct cgggggcgaa cccccacgtc gcgccaatca
     1381 attgcgagtg cccctgatag atccgctgtt cgagcgccgg ccgcggatgg aggtacaccg
     1441 gcacagcgag cgaggacacc gcttcccaga acggaaggaa cgcgggctcg tcgaggtatg
     1501 ctccgccgcg cccatcggcc acgtcggtgt agccgttgac catcgcccct ttcaaaccga
     1561 gccggctgac gcaccggtcc agttcttggg cggcggcagc gggatcctgc agcggaacac
     1621 tggcaaagcc ggcaaaacgc gtgggatggc tggcgatccg cccggcgaga aaatcgttga
     1681 cgtcgcgcgc ggccgcgacg gcatccgccg cgtcgacgat cccctgaacg ccgggaacag
     1741 tgtgagacag gatacacacg tcgatgccgc cggcatccat cgcggcgatg cggcgctctc
     1801 cgacgtcgga caggcgctcg cccaccagcg ccgaccacgc ctggtcgtag tccatcgact
     1861 tcaccaggct cgtgatgtca acgtgcgccc gatcgccggg cccggcggtc aggtagttga
     1921 agtgttcctc gacagcgatc ttcggcagcc gcggccgcgg cggcggcgcg ccgattgccg
     1981 ggaggtcgtc caacgttctc agcgagtcgc gcccggcctc gcctcctcgg cgcgcgccgc
     2041 gctgaggatg ttgcgcatgg cgtagttccc ctcgtggcac gcgtattcga acagcggctg
     2101 ctcggggtcg gccgtcagcg tcatcccgaa ggtccacggc ctggtccatg tctccgggtc
     2161 gtcaaccgtc atcgaccagt cgatcttgtt cttggcgacc ggcttgaagc gctcggtcag
     2221 cttcatcttc gccgagccgt tcctgtacac gctgcgcggg tcgaagccca cggtctcgac
     2281 aacgagcgtg tcgccctccc agcggccacg cgcatctccc atgtgtgacg gaaggccgga
     2341 gcgtggagag ttgtcgagcc ggatgatccg cgtttcgtgg atcatctcgt agcggatggc
     2401 cacgattccg ggggcctggg tgatgtcgta cgcgttgccg taaatggccg gcatcatcga
     2461 gcccggaatc ccgcgcgaga tgcagcgatc gtagaggctt cgatcgagcc aggagtcggc
     2521 gcgtccgcct tcagcgaatc cgccgccgcg gcctccccgg ccgccgatgc ggcccgcgat
     2581 ccgcgcctgc gccgccgccg tctgcgccgg gatgcggccg tcgggaggat cgatcaccat
     2641 ccaggcgcgg ctgttcttcg cgccgtagtg ctcgtaccaa tgcacgggcc cggcgccggt
     2701 gtcgcggccg ccgatcgccg ccgcgccgcg caggcgcgct tcatcgcgcg ccttgtccag
     2761 ctcggcgagc tcggagtcgt ccacctgctc gagttgtttg ccggcgagcg cgtcggggcg
     2821 ctcgaaggga ataccgtttt cgctgacgtt ggtccaaccg ccctggagat cgggatcgcc
     2881 ccacggcgtc cgcggcgcga cgtacggctt ggcggtagcg gcgggtgcag acaccgcgct
     2941 acggcctgtc tgctgcgaat ccaggctggc actcgtcccg gccacgacac ccacggcgac
     3001 cccggcaaca gcgagccaat gccttgtcat agacgcccct tttgaaagct ggggaaagtc
     3061 tacacccagg cagggctccc gtgggttgat gccgtcagcg ctggtccggc cctgccttgc
     3121 cccgccggag ctcgtcgccc aggatgatcg cctcggcgat ctccttcttc gacttgcgtc
     3181 gctgccggct ctgccgctgg atggtcaggt acgcgtcttc ctcggtgagg ccgaggtcgc
     3241 gctgcaggat gcccttcgcg cgatcgagga gcttcctcgt ctcgaggcgc tcgaagagct
     3301 gcgagttctc catctcgagc cgcgccgtct cgatctcggc gccgaccagg aatccaatcg
     3361 tcgagatgag ctgaatctcc cgctgcgtat gcgtgtgcgg ctgccggtgc tggaggttga
     3421 tcacgcccac cggcttgccg cgcgacagca cgggcaccga cagaaacgcc tcgtaccgat
     3481 cctccggcag ctcgttgaag gtctggaagc ggggatcctc gaaggcgtgc cggcccacgg
     3541 cgaccggctg acgatgcgcg gcgacccacc cggtgatgcc ctgccccagg cggagcgtga
     3601 gccggtccac gacgtcggga tgcggcgtct tcgaggcgcg aagcacgagg tcctcgtcct
     3661 gcaggacgta gacgaagcac gagtcgcagt gcacgagcgc cgacaccacg tccacgaccc
     3721 gggacagcac gtcgtggagc ggatcggccg cggcgaggcg gctgccgatc tcgtggagga
     3781 gatcgatgtc ggacggcgag gtcatcacac cgctatcgaa gatacacagc gcacgcctct
     3841 tcaacaaggg gggtcgtgcc gacgttcatg tatgacttgc actttcatgc aacatatttg
     3901 aaggatccgg gagggccacc atcaccaggc cgtcctcgac gcgggcggga taggtggtga
     3961 cgcagcgctc gctccccgcc gggcgctcga cgctcccgct ggccaggctc actttccacg
     4021 aatgcaacgg acagacgacc gacgcccccg tcacgatgcc gtcacacagc ggaccgccgg
     4081 cgtgcgggca gcggttgtcg gtcgccagga agcggtctcc caggttgaag atcgcgatct
     4141 cccggtttcc gatctgcacg gcccggcctt cccgctcggg aatgctctcg atcgacgtga
     4201 cgcgaatcca gcggtgcgcg gtcatgcgag caccttgtcc acgaccgaga tcggctcgaa
     4261 ctgcgtcggg tgccgcggcg cctgcccttc gtgccacgcg tcgcgcgcgc gcgccttgga
     4321 tttgcgcagc cgatcgagca gcgcgctcct cgtcgcgtcg gtcgcgtaga cggtgtcctt
     4381 ccgcaccttc tcgatgccga gccgctcgac gaagtcgtag gtgcgctcga ggtaattggc
     4441 gttctcgcgg tagtactgga agaacagctc ggccgcctcg agcgcctcct cggtcgtctc
     4501 gaccgtcgtc agcagatcgg ccttgcgcac gctcttgccg gccgctccgc cgacgaccac
     4561 ctgccagccg ccttcgatgc ccacgagccc gatgtccttg acggtggcct cggcgcagtt
     4621 tcgcggacat cccacggcgc ccatcttcac cttgtgcggc gtgtacaact gctcgaaccg
     4681 ccgctccatc tcgatacccg cggcggtcga gtgctgcgtg ccgaaccggc agaactcggt
     4741 gccaacgcac gtcttcacca tgcggacgcc cttcgtgtag gcctgccccg acggcatgcc
     4801 gagatcggcc cagaccttcg gcaggtccgc cttcctgatc ccgagcagat cgattcgctg
     4861 gctcccggtg atcttcacca tcgggacctg gtacttctcg gcgacgtcgg caatgcgccg
     4921 gagctcggca gggttggtca cgccgccacg gatgcgcggt acgacggaaa acgtgccgtc
     4981 cttctggatg ttcgcgtgca cgcgatcgtt gatgaaccgc gcgga
//
LOCUS       DBNGSH010000110        10931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_856351, whole genome shotgun sequence.
ACCESSION   DBNGSH010000110 DBNGSH010000000
VERSION     DBNGSH010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_856351"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="AC16CY_02395"
     CDS             <1..882
                     /locus_tag="AC16CY_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3352655.1"
                     /translation="AVPVVSDGVIYTFGAEGQLHAVDLAKGTRLWSEDTMKRFGVPKG
                     FFGAGGSPLVEGGRVIANIGGDKAGLVAFDGKTGKVVWTATDDDASYSSGVMATFGGV
                     RSALFLTRDHLIAVDPATGATRFQRAWRARQAASINAATPLVIGDQIFVSAEYGPGAG
                     LLRVNGSQLVDVWTSNDVLSNHYATSVHHNGYLYGFHGRQEFGPSLRAVELRTGNVRW
                     NLDQFRAGSVLIAGDRLVVMREGGELLLAPASPQAFKPTARAQILQGVTRPYPAIADG
                     FLYVRNENTLLCLDLRR"
     gene            895..1530
                     /locus_tag="AC16CY_02400"
     CDS             895..1530
                     /locus_tag="AC16CY_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3352656.1"
                     /translation="MKASALCVLVIAALAPLMGAPPPQRAEADAAFDQALKDFVAGRI
                     VESVAGFDRVVALVPSAAPQLWQRGIALYYAGRYADCRKQFELHRTVNPNDVENPVWH
                     FLCVSHQEASPQKARAALLPVGKDGRRPMPQIYEMFRGTLTPQAVLAAAGSNLEAQFY
                     AEAYVGLYYEGTGNLEEGRVHLRNAAAEKFQPFGGYMHRVVQLHPLLTGGR"
     gene            complement(1952..3175)
                     /locus_tag="AC16CY_02405"
     CDS             complement(1952..3175)
                     /locus_tag="AC16CY_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3352657.1"
                     /translation="MAVLMLAVSAVCARAGDWPQFRGPSGQGHSSDVGLPLEWSESRN
                     IAWKAPVGEGWSSPVVSGGRVWVTTVSESREGRRVVSSLRAVALDAATGREVVNVEVF
                     RLENPGSINEKNSRASPTPIVDGDRVYVHFGADGTAAVSKDGKILWTVRLRYDSQHGN
                     GGSPVLYQDLLIVNCDGWGEEAYVIALDTSTGKTRWKTSRRRPWSHAYSTPLIIRAGD
                     EDQLVSIGAYRTAAYEPLTGREIWRVNYDEGFSNVPRPVYGGGLVYIATGFQVPSLMA
                     VRVDGRGDVTRTHVAWTLARGAPYTPSPLLVGDELYYLSDTGVLSCVDAKTGEMRYQQ
                     RLVGNFSASPVFVDGRIYVLSEEGVTSVLAPGKQFKALATNRLDGRTLASMAVADGSF
                     FLRSDTHLYRIAAKQ"
     gene            3336..4625
                     /locus_tag="AC16CY_02410"
     CDS             3336..4625
                     /locus_tag="AC16CY_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3352658.1"
                     /translation="MPTSRKTDPTRWRISRETRQLLTAAVLALAALWALARIRYPAGP
                     PTPNPIPSLLSLAPMPRFSNLAGEIEDLQKSLSPFWLVAQVSVSSGDTGTARVRSLAA
                     LRLRDDAAVVLLGDNARLSDNDSIIAADSASGLTLFRTRGEASDSVPAPWTPPDLGSP
                     HYLMATSASPAGISLNPVLVGSLYPVRSAAWSGPIWVVPEGTYLETGAFLFTTSGELA
                     GVAALEPDGTVIVPGDVVLADSMRLLERGSASVVDVPIEVQPLTPALARATGARTGVV
                     VAWVDPEGAASARLKVGDVIEAVDGQPAASVRDWDVLTRRLSTPGVNLRVWRRGSFID
                     VPLSLPALESEAPAPAIGMTMRDLPGVGSSVLEVVRGSPADRARLLAGDVITLAGGAS
                     APTPEHIRNTFAAARAGEPILIALTRGRNHLVIALVK"
     gene            4622..5752
                     /locus_tag="AC16CY_02415"
     CDS             4622..5752
                     /locus_tag="AC16CY_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3352659.1"
                     /translation="MMLNLELDRLRVAAWERAPWHARFGLSWSTGFVPVVLILLLGAA
                     LGPSGVALLTPRVLEVLNPVVPVAVAALGILAGLECTRPASPFRWALLAKASLQSTLT
                     GVTVATGVWFLAPNLLDVSGFDAWTVTLVSGVCASMSASLPATDPDRLRPPTMRIRDL
                     DAILPTLAGMFVLAAVRSPSATASITLTAQAALLSLVISAAAWLLLADTSPGTEQRVF
                     AIAALLLVGGIADYLSLSALASGLLAGLFWGLVGGAARDCFERDIGYLRHPLVVLMLL
                     IAGAQAALSPAILALAAAYLALRVVGKLVGGTLVRRLPPVRQPEGLGTSLLPPGVFGI
                     AFALNAAPLLGPPAMPLLAAVVLGTIGSQLVAAVLRPQEALE"
     gene            5749..8502
                     /locus_tag="AC16CY_02420"
     CDS             5749..8502
                     /locus_tag="AC16CY_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HIR3352660.1"
                     /translation="MKQFAAFGLAVAAVLGARRLGSSEAGSVGAALAIGFALLGAWVA
                     GDLLRRLHLPRLTGYILFGVLIGPGVGGVITESMAVHLQFITGLATTLIALIAGLTLN
                     FERLGEQFTGIVRLTATTLGVAMLGIGAVAWLAWPWLPIAPEASGLTRLAIVSLLVVT
                     TVSFSPTMTAAVTTETGARGRLSETVLTVVVLADLALLVLFSLAMLFARIVVGTTAGD
                     GGLIGRFVWELGGAVAFGVLVGSVFALYLRHIGREVTLVIIAVCALLSQVGSTQQFEP
                     LLAAVAAGLVIENLAIAQGDALKAAVQAAAPPVLVIFFVAVGTSLRLDVLVESGVIVL
                     GLSVLRILLIRIGVRAGVRSSGIERSVGSHIWTGLISQAGITLGLASVVAAEFGDWGM
                     RIQTLVVALIAIHELVGPLLFKYGLARGGEMAAAAPRPLVVVSNREPYLHRENEQGQI
                     VATAATGGVAVALDALMRERHGVWVAHGSGSADRRVVNARDKVAVPPDGPSYELRRIW
                     LEDGTYNAYYGGFANEGLWPLCHLVDVRPQFRTEDWAAYRDVNQRFADAVSEEVGSAH
                     TPIFIHDYHLALVAPALRARLPHARTAFFWHIPWPYPDRFRICPWSSEILRGLLENDL
                     LAFQLERDRRNFLLAAEEEAGVEVEYEASRVRFGDRHATVISVPIGVDYDRIQAITSA
                     PSLLEEQQRLRDLFGLSADVIGVGVDRLDYTKGIPERLAAIDVLLTRRPQLRGKLTFV
                     QIGVPSRTEIDSYAAIEAEIGEQISALNMRHAVPGGAPAVYYHKTPLTIASLAALYRL
                     ADFCIVSSLHDGMNLVAKEFVASRDDDDGVLVLSGLAGAAQELNEAVIINPYDVDGFA
                     DGLLRAIDMPADERQARMRAMRLVVAGRNVFGWASDILEGLESLWTKPLHYSVRRWED
                     MSV"
     gene            complement(8567..8803)
                     /locus_tag="AC16CY_02425"
     CDS             complement(8567..8803)
                     /locus_tag="AC16CY_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352661.1"
                     /translation="MWWFLQQNPIIVDVGSQKAPTGDITIDFVVGMFALAGVLLLLAT
                     AGCLIVAAVVIIYKRRHPPLDAEGGSTSHTRLRI"
     gene            complement(8808..10328)
                     /locus_tag="AC16CY_02430"
     CDS             complement(8808..10328)
                     /locus_tag="AC16CY_02430"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR3352662.1"
                     /translation="MHSAHKTPAVALTARSMAEVDADLLVIPSFSDDDLADEPDLDRA
                     SGGEVARARSRGELKGKPYEVIATTLSGWKTSRAVLVGAGPRADVTADHLRRIAIIGG
                     LAARQRRLARVAIAFRPVAGVTPIVAAQVLAEGGVIANFEAAYRKTDQPSTWLEHVEV
                     RIAGSHAGAQEALDRGRVLGEYTNQARELANAPGNELTPRVFAERAVEIATSAGLAVE
                     VLDEKQIATLGMGMLLGVARGSHEPPRLVVLRHEPPNAASDVVLGLVGKGITFDTGGI
                     SIKPAENMDRMKDDMSGGAAVICAMSAISRLRLPVRCIGIVPMTENMPGGRAIKPGDV
                     LTSAAGKTVEILNTDAEGRLILGDGVWYARQRGATHLIDVATLTGACVVALGKTTSGL
                     FGTPPAWVEHVRRATERAGDRSWPMPVFDDYKEQLKSEIADFSNTGGRPGGAITAALF
                     IKEFAGDRPWVHMDIAGTAWAEDTRPYQPKGATGVAVRTLVELARDSASWGAADLH"
     gene            10454..10894
                     /locus_tag="AC16CY_02435"
     CDS             10454..10894
                     /locus_tag="AC16CY_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR3352663.1"
                     /translation="MACSRRACSAVAVVLGAVLCTDAASMAQSDSEARKPSLSLRVTP
                     SLGFTPLKARFAVDVRGGDDDFEDFYCPTIEWSWGDGTVSSNSEDCDPYEAGKSLIRR
                     RFSAEHVYRQPGTFRVSFRLKQQKTRVVGTANTILQVRAGGSQN"
BASE COUNT         1620 a         3777 c         3661 g         1873 t
ORIGIN      
        1 gcggtgcccg tcgtctccga cggcgtcatc tacacgttcg gcgcggaagg ccagctgcac
       61 gccgtcgatc tcgccaaggg cacgcgcctc tggagcgagg acacgatgaa gcgcttcggc
      121 gtgcccaaag ggttcttcgg cgccggcggc tcgccgctcg tcgaaggcgg ccgcgtcatc
      181 gccaacatcg gcggagacaa agcgggactc gtggcgttcg atggcaagac gggcaaggtc
      241 gtctggacgg ccaccgacga cgatgcgagc tactcgtcgg gagtgatggc gacattcggg
      301 ggcgttcgat cggcgctgtt cctcacccgt gatcacctga tcgccgtgga tcccgccacc
      361 ggggcaacac ggtttcagcg agcgtggcgg gcgcggcagg ccgcgtccat caacgcggcg
      421 acgccgctcg tcatcgggga ccagatattc gtgtccgcgg agtacggccc cggggcgggc
      481 ctgctgcgcg tgaacggctc gcagctcgtg gacgtgtgga cgtcgaacga cgtgctctcc
      541 aaccactacg ccacgagcgt tcatcacaac ggttacctgt acggctttca cgggcgtcag
      601 gagttcgggc ccagcctcag agcggtcgag ctccggacgg ggaacgtgag atggaatctg
      661 gatcagttcc gcgcgggcag cgtcctcatc gcgggagacc ggctggtagt catgcgcgag
      721 ggaggcgagc tcctgctcgc gccggcgtcg cctcaggcgt tcaagccgac ggcgcgtgcc
      781 cagatcctgc aaggggtcac gcggccgtac ccggcgatag cggatggatt cctctatgtc
      841 cggaacgaga acacgctgct ctgcctcgac ctgcggcgct gaagggacgg gacgatgaaa
      901 gcaagcgcgc tttgcgtgct cgtgatcgcg gcgctcgctc ccctgatggg cgcgccgccc
      961 ccgcaacggg cggaagccga tgccgccttc gatcaagccc tcaaggactt cgtggccggc
     1021 cggatcgtcg agtcggtcgc cggcttcgat cgcgtcgtcg cgctggtgcc cagtgccgcg
     1081 ccccagctct ggcagcgcgg catcgcgctg tactacgccg ggcgctacgc ggactgccgc
     1141 aagcagttcg agctgcacag gaccgtgaac ccgaacgacg tggagaaccc ggtgtggcac
     1201 tttctctgcg tctcgcacca ggaggcgtcg ccgcagaagg cacgggcagc cctgctgcct
     1261 gtcggcaagg atggacgccg gcccatgccg cagatctacg agatgttccg cggcacgctt
     1321 acgcctcagg ccgtgctcgc cgcagccgga tcgaacctcg aggcacagtt ctacgccgag
     1381 gcgtacgtcg gcctgtacta cgaaggcacc ggcaacctcg aagaggggcg cgtgcacctg
     1441 cgcaacgccg ccgctgaaaa gtttcaaccc tttggcggct acatgcacag ggtggtgcag
     1501 ttgcaccccc tgctgaccgg cggacgataa gaaggcccca ggaatcaggg ctcaggcctc
     1561 agggctgggc caggtaagca agccctatta atcaggcctg gggccttgtc ccttgcccag
     1621 ccctcgccat cgacccattc gctttttgag tcagtgaaca cggcggcggc acgactgatg
     1681 tgacctggag cgtcggcgtg tcgcaagccc gtcacgcgac agctcgccac cggcgttgga
     1741 ggcgcggagg agaagcgagg gcgtcaggcg atcgcgacgg aggagcgccg acagaccgtc
     1801 agcccgaacg ccgaagatgc cggtggccgg gcgccgcgac ccgccgcgcg gaaggacacg
     1861 tcagccgggc cgccgccgcg gcctcgctca acggtgaagc ctcagacatc ccggaaggac
     1921 gtcataacgc atcaggccag cactcacagg cctactgttt ggctgcaatt cggtacaggt
     1981 gagtatcact ccgaaggaag aacgatccgt ccgcgacagc catcgacgcc agcgtccttc
     2041 cgtcgagacg attggtggcc agagccttga actgctttcc gggcgccagc accgacgtca
     2101 cgccttcctc gctgaggacg tagatgcgcc cgtcgacgaa caccggcgac gccgagaagt
     2161 tgccgaccag acgctgctga taccgcatct ctcctgtctt ggcatcgacg cacgacagca
     2221 cgccggtgtc gctcaggtaa tagagctcgt cgcccacgag cagcggtgac ggcgtgtagg
     2281 gcgcccccct ggcaagcgtc cacgcgacgt gcgtgcgcgt cacgtcgccg cgcccgtcca
     2341 cgcgcacggc catgagcgag ggcacctgga atcccgtggc gatgtacacg aggcctccgc
     2401 cgtacacggg cctcggtacg ttggaaaagc cctcgtcgta gttgacgcgc cagatctcgc
     2461 ggcccgtcaa cggctcgtag gcggccgtcc ggtacgcgcc gatggagacg agctgatctt
     2521 cgtcgccggc gcggatgatg agcggcgtcg aataggcgtg gctccacggc cggcgccggg
     2581 acgttttcca cctcgtcttg cccgtgctcg tgtcgagcgc gatgacgtag gcctcctcgc
     2641 cccagccgtc gcaattgacg atcagcagat cctggtagag cactggcgag ccgccgttgc
     2701 cgtgctgcga gtcgtagcgc aggcgcacgg tccacaggat cttgccgtcc ttcgacacgg
     2761 cggcggtccc gtccgccccg aagtgcacgt acacgcgatc gccgtcgacg atcggggtcg
     2821 gagacgcgcg gctgttcttc tcgttgatgc tgcccgggtt ctcgaggcgg aagacttcaa
     2881 cgttgaccac ctcgcgcccc gtggccgcgt cgagcgccac ggcgcgaagc gacgacacca
     2941 cgcggcggcc ctcgcgggac tccgagaccg tggtcaccca gacgcgcccg ccggagacaa
     3001 caggggacga ccatccttcg ccgactgggg ccttccacgc gatgttccgc gattcgctcc
     3061 actcgagcgg cagtcccaca tcggaggaat gcccctgtcc ggagggccct cgaaactgcg
     3121 gccagtcccc cgcgcgtgcg cacacggcgg acacggcgag catgaggact gccatgggcc
     3181 accgcgagcg tgagtgaaac actggcatag cctgacgcgt tctcactaga atgagtagca
     3241 cacgatgacc cctcggcagc gtcaatcggc tggatccgat agctcgaccg tagcccgcgc
     3301 cacgagaccg cttcgtaggc gcgcgatcga ggcgtgtgcc gacctcacgc aagactgatc
     3361 cgacgcgctg gcgcatctcc cgcgaaacgc gtcagttgct gacggcggcc gtcctcgcgt
     3421 tggcggcgct gtgggcgctc gcacgcatac ggtacccggc cgggcctcct acaccgaacc
     3481 cgattccttc gctgctgtcg ctcgccccga tgccgcggtt ttcgaatctc gccggagaga
     3541 tcgaggacct tcaaaagagc ctttcgccct tctggctcgt cgcgcaggtc tccgtctcat
     3601 caggcgacac cggtaccgcc agggtgcggt cgctggcggc tctcagatta cgggatgatg
     3661 cggctgtcgt gctgcttggg gataacgcac gcctgtcgga caacgactcc atcattgccg
     3721 ccgatagtgc cagtggcctc accctgtttc gtacccgcgg ggaggcgtcg gactcggttc
     3781 ccgcaccctg gacgccgcct gacctcggca gtcctcacta tctcatggcc acctccgcgt
     3841 cgccggctgg catctcgttg aatcccgtgc tggtcggctc gctgtatccg gttcgcagtg
     3901 ccgcctggtc cggccccatc tgggtcgtcc ccgaaggcac gtatctcgag accggcgcct
     3961 tcctcttcac gacgagcggt gagctggctg gcgtcgcagc actcgagccc gacggcaccg
     4021 tcatcgtccc cggagacgtc gtgcttgcgg actccatgcg cttgctcgaa cgtggaagtg
     4081 cgtccgtcgt cgacgtgccg atcgaggtac agcccttgac gcccgcgctg gcgagagcga
     4141 caggcgcacg tacgggcgtc gtcgtggcgt gggtggatcc tgaaggggcc gcgtcggcgc
     4201 ggctgaaggt tggcgacgtc atcgaagcgg ttgacggcca gcccgcagct tccgtgcgcg
     4261 actgggacgt cctgaccaga cggctctcga cgccgggcgt caacctgcgt gtctggcggc
     4321 gaggcagctt catcgacgtt ccgttgtcgc tgccagcgct cgagtcggag gctcctgcgc
     4381 ctgcgatcgg gatgaccatg cgtgacctgc ctggcgtcgg ctcgtccgtg ttggaagtcg
     4441 tgcgcggctc gccggccgac cgggcgcgcc tgctcgccgg agacgtcatc acgctcgcgg
     4501 gcggcgccag cgcgccgacg ccagagcaca tccggaacac gttcgcggcc gccagggctg
     4561 gcgagcccat cctgatcgcg ctgactcgag gacggaacca cctcgtgatt gccctggtga
     4621 aatgatgctg aacctcgagc tggatcggct ccgtgttgcg gcctgggagc gcgcgccctg
     4681 gcacgcacgt ttcgggctga gctggtcaac gggcttcgtt cccgtcgtgc tcatcctgct
     4741 gctcggcgcc gccctcgggc cgagcggcgt ggcgctgctg actccgcgtg tgctcgaggt
     4801 tctcaatccg gtcgtcccgg tcgccgtcgc ggcgctcggc atcctcgcgg gcctggaatg
     4861 cacccggccg gcgtccccct ttcgatgggc gctgctcgcc aaagcgagtc tgcaatcgac
     4921 gctcaccggc gtgaccgtcg cgaccggcgt ctggttcctt gcgccgaacc tgctcgacgt
     4981 gagcgggttc gatgcatgga cggtgacgct ggtctcgggg gtgtgtgcgt cgatgtcggc
     5041 ctcgctgccc gccacggatc cggatcgcct tcgccccccg acgatgcgca tccgcgatct
     5101 cgacgccatt cttccaacgc tggccggcat gttcgtcctg gcggcggtgc gttcgccgtc
     5161 ggccacggcg tcgatcactc tcacagcgca ggcggctctt ctgtcgctcg tgatctcggc
     5221 tgccgcctgg ctcctcctcg ccgatacgtc accaggcacg gagcagcgtg tgtttgcaat
     5281 cgcggcgttg ctgctcgtcg ggggaatcgc cgactatctc tccctgtcgg ccctcgcgag
     5341 cggcctgctg gcgggcctgt tctggggact cgtgggcgga gcggcgcggg actgcttcga
     5401 gcgggacatc ggctatctgc gtcatccgct ggtcgtgctg atgctcctga ttgccggcgc
     5461 gcaagcggca ctgtcccccg caatcctcgc gctggcggcc gcgtacctcg cgctacgggt
     5521 cgtgggaaag ctggtaggcg gaacgctcgt gcgtcgcctc ccgcccgttc ggcaaccaga
     5581 gggcctcggc acgtcgctgc ttccccctgg cgtcttcggg atcgcgttcg cattgaacgc
     5641 ggcgcctctg ctcggtcccc cggcgatgcc gctgctggcc gcggtcgtcc tcggcacgat
     5701 cgggtcccag ctggtggcgg ccgtgctccg tccgcaggag gcgctcgaat gaagcagttc
     5761 gcggccttcg gcctcgccgt cgctgccgtt ctgggagcgc gcaggctcgg atccagcgag
     5821 gccggatcgg tcggcgctgc gctcgcgatc ggcttcgcgc tgctcggcgc gtgggtggcg
     5881 ggcgatctcc tgcggcgcct ccacctcccc cggctcaccg gctacatcct cttcggcgtc
     5941 ctcatcggcc ccggtgtcgg cggcgtcatc acggagtcga tggccgtcca tctgcagttc
     6001 atcaccggcc tggcgacgac gctcatcgcg ttgattgccg ggctgacgct caatttcgag
     6061 cggctcggcg agcagttcac cggcatcgtt cgtttgacgg caaccacgct cggcgtcgcg
     6121 atgctcggca tcggagccgt ggcgtggctg gcgtggccgt ggctcccgat cgcgccggag
     6181 gcctcgggcc tgacgcggct ggcaatcgtg tcgctcctcg tggtgacgac cgtcagcttc
     6241 tcccccacca tgaccgcggc ggtcaccaca gaaaccggcg cccgcgggcg gttgagcgag
     6301 accgtgctca cggtcgtcgt cctcgccgac ctcgccctgc tcgtgctgtt ttcactcgcc
     6361 atgctgtttg cccgcatcgt cgtcggcacg acggccggtg atggcgggct gatcggccgt
     6421 ttcgtgtggg agcttggcgg ggctgtcgcg ttcggcgtcc tcgtcggctc ggtgttcgcg
     6481 ctgtacctgc ggcacatcgg gcgcgaggtc acgctcgtga tcatcgcggt ctgcgcgctg
     6541 ctcagccagg tgggaagcac tcagcagttc gaacccctgc tcgcggccgt ggcggccggc
     6601 ctcgtgatcg agaacctggc gatcgcccag ggcgacgcgt tgaaggcagc ggtccaggcc
     6661 gccgctcccc ccgtgctcgt catcttcttc gtcgccgttg gcacgtcact gcgcctcgac
     6721 gtgctcgtcg agagcggcgt gatcgtgctc ggcctctccg tgcttcgaat cctcctcatc
     6781 aggatcggcg tgcgcgcggg ggtccgatcg tcgggaatcg aacgctcggt cggcagccac
     6841 atctggaccg gcctgatttc tcaggccggc atcacgctcg gcctggcgtc agtggtggcg
     6901 gccgaattcg gcgactgggg gatgcgaatt cagacgctgg tcgtcgcgct gatcgcgatt
     6961 cacgaactgg tcggcccgct gctcttcaag tacgggctcg cgcgcggcgg cgagatggcg
     7021 gccgcggcgc cgcgccctct cgtcgtggtc tcgaaccgtg aaccgtacct gcatcgggag
     7081 aacgagcaag gccagatcgt cgccacggcg gccaccggcg gcgtggcggt cgcccttgac
     7141 gcgctcatgc gcgagcgcca cggcgtgtgg gtcgcgcacg gctccggctc ggcggatcgg
     7201 cgcgtcgtca acgcgcgcga caaggtggct gttccacccg acggtccgtc gtacgagctc
     7261 cggcggatct ggctcgaaga cggaacctac aacgcgtact acggcggctt cgccaacgag
     7321 gggctctggc ctctctgcca cctcgtcgac gtgcggcccc agttccggac cgaggactgg
     7381 gctgcgtatc gagacgtgaa ccagaggttt gcggacgccg tcagcgaaga ggtgggctcg
     7441 gcacacacgc cgattttcat ccacgactac cacctcgccc tcgtcgcgcc cgcgcttcga
     7501 gcgaggctgc cccacgcccg gacggcattc ttctggcaca ttccgtggcc gtacccggat
     7561 cgattccgga tctgcccgtg gtcgtcagag attctccgag gcctgctcga gaacgacctg
     7621 ctcgcgtttc agctggagcg cgatcgccgg aacttcctgc tcgccgccga ggaagaagcg
     7681 ggcgtcgaag tggagtacga agcctcgagg gtccggttcg gagaccggca tgcgaccgtc
     7741 atctcggtcc cgatcggagt tgactacgat cgcattcaag cgattacctc ggcgccgtcg
     7801 ctgctcgagg agcagcagcg cctccgggac ctcttcgggc tgtcggccga cgtcatcggc
     7861 gtcggcgtgg accggctcga ctacacgaaa gggatccccg agcggctcgc ggcgatcgac
     7921 gtcctgctca ctcgacgccc gcagctacgc ggcaagttga cgttcgtgca aatcggcgtg
     7981 ccctcgcgga ccgagatcga cagctacgcc gccatcgagg ccgagatcgg cgagcagatc
     8041 tccgcgctca acatgcgtca tgccgtgccg ggcggcgccc cggccgtcta ctaccacaag
     8101 acgccgctca cgatcgcgag ccttgccgcc ctctaccgcc tggcggactt ctgcatcgtc
     8161 agctcgcttc acgacggcat gaacctcgtg gccaaggagt tcgtcgcgtc gcgcgacgat
     8221 gacgacggcg tgctcgtgct gagtggtctg gcgggtgccg cgcaggaatt gaacgaagcc
     8281 gtgatcatca atccttacga cgttgacggc ttcgccgacg ggctgcttcg cgcgatcgac
     8341 atgccggcgg acgaacgcca ggcgcgcatg cgcgcgatgc gcctggtcgt cgccggacgc
     8401 aacgtgttcg gctgggcctc cgacattctg gaaggactcg agagcctgtg gaccaagccg
     8461 ctgcactact cggtgcggcg gtgggaggac atgtcggtct agttctcgta gcctgggccg
     8521 gtctaaagac cggcccctac ggcgtccaaa caagtgagtt ccggaatcag atccgcagcc
     8581 tggtgtggct cgtcgacccg ccttccgcgt ccagcggcgg atgccggcgt ttgtagatga
     8641 tgaccacggc ggccacgatc aggcagcccg ccgtggcgag gagcagcagc acacccgcca
     8701 gcgcgaacat gccgacgacg aagtcgatcg tgatgtcgcc ggtcggtgcc ttctggctgc
     8761 cgacgtcaac gataatcgga ttctgctgga ggaaccacca cacggggtta gtgtagatcc
     8821 gcggcacccc acgatgcgga gtcgcgcgcg agttcgacca acgtccgcac ggccacgccc
     8881 gtcgcgcctt tcggctggta gggcctggtg tcttcggccc acgccgtccc ggcgatgtcc
     8941 atgtggaccc acggccggtc gccggcaaac tccttgatga agagcgctgc ggtgatggcg
     9001 ccgccgggcc gccccccggt gttgctgaaa tcggcgatct cgctcttgag ctgctccttg
     9061 taatcgtcga agaccggcat cggccacgac cgatctcccg cccgctcggt cgcccgccga
     9121 acgtgctcga cccacgccgg cggcgttccg aacagcccgc ttgttgtctt gccgagcgcc
     9181 acgacgcacg cgccggtgag cgtggccacg tcaatcagat gcgtggcccc ccgctggcgc
     9241 gcataccaca cgccgtcgcc caggatcagc cgcccctcgg cgtcggtgtt gagaatctcg
     9301 accgtcttcc ctgcggcgct cgtcagcacg tcgccgggct tgatggcgcg gccgcccggc
     9361 atgttctcgg tcatgggtac gatgccgatg cagcgaaccg gaaggcggag acgcgagatc
     9421 gccgacatgg cgcagatcac ggcggcgccg cccgacatgt cgtccttcat cctgtccatg
     9481 ttctcggccg gcttgatcga gatgccgccg gtgtcgaacg tgatgccctt tccgaccagg
     9541 cccagcacga cgtccgacgc ggcgttcggc ggttcgtgcc gcagtacgac gagacgcggc
     9601 ggctcgtggc tgccgcgcgc gacgccgagc agcattccca ttccgagcgt ggcaatctgc
     9661 ttttcgtcga gcacctcgac cgccagcccc gccgaggtgg cgatctcgac ggcccgctcg
     9721 gcgaagaccc gcggcgtgag ctcgttgccg ggggcgttgg ccagctcgcg cgcctgattg
     9781 gtgtactcgc cgagcacacg gccccggtcg agcgcctcct gcgctcccgc gtgcgagccg
     9841 gcgatccgca cctcgacgtg ctcgagccac gtcgatggct gatccgtctt ccggtacgcg
     9901 gcctcgaagt tcgcgatgac gccgccttca gcgagcacct gcgcggcgac gatcggcgtg
     9961 actccggcaa ctggccggaa cgcgattgcg acccgcgcga gccggcgctg ccgggcagca
    10021 aggcccccga tgatcgcgat gcggcgcaga tggtccgccg tgacgtcggc gcggggaccc
    10081 gcgccgacga gcacggcgcg cgacgtcttc catcccgaca acgtcgtggc gatgacttcg
    10141 tacggcttgc ccttcagctc gccccgcgat cgcgcgcgcg ccacctcgcc accggaggcg
    10201 cgatcgagat ccggttcgtc ggccaggtcg tcgtcgctga aagagggaat caccagaagg
    10261 tccgcgtcga cctcggccat ggaccgcgcc gtcagagcga cggcgggcgt cttgtgcgcg
    10321 ctgtgcatcg agtcgaactc cccgtcccgg cggctgggag gcgtggccgg gtcacaccgt
    10381 gttcacctga tgcttcgagc ttaaccccag gctgtggagt aaaatcgact tcagtctgcc
    10441 ctggtgtaac gagatggcgt gctcacgacg cgcgtgcagt gcggttgcgg tcgtcctcgg
    10501 cgccgtgctg tgcacggacg cggcgtcgat ggcgcagtcg gacagcgaag ccagaaagcc
    10561 aagtctatca ctcagggtca ctccgtcgct cggcttcacg cccctgaagg cccgcttcgc
    10621 ggtggacgtt cgcggcggag acgacgactt cgaggacttc tactgtccga cgattgaatg
    10681 gagctgggga gatggaaccg tctcatccaa ctcagaggac tgcgatccct acgaggccgg
    10741 caagagcctc atccggaggc ggttctcggc tgagcacgtc taccggcagc cgggcacctt
    10801 ccgggtctcc tttcgattga agcagcagaa gacccgcgtg gtcgggaccg ccaacaccat
    10861 cctgcaggtc agagcgggcg gctcacagaa ctgacaacgt cggcaccggc ggcggcgtcg
    10921 cgcgcgacgc c
//
LOCUS       DBNGSH010000111         5257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3913004, whole genome shotgun sequence.
ACCESSION   DBNGSH010000111 DBNGSH010000000
VERSION     DBNGSH010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3913004"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="AC16CY_02440"
     CDS             complement(<1..1240)
                     /locus_tag="AC16CY_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease-associated domain-containing protein"
                     /protein_id="HIR3352664.1"
                     /translation="MVPGLVARPAFAQPGVEAMTDRIRAHLAQHAAFGDKFSGGPGDL
                     ATARWIAGRLRQSGYRVEESEFQAPFFVKRTARLTSGTTSVDVVPQAPVIPTGPAGVT
                     APLALVEGGAVGDVKNRLAIVVLPFGRHAALFPDRGIGQTVKQVAAAGARAIVMVTTG
                     PSGEAVALNSPEEPFVPIPAAVIAPRRATALVDAARSRSQATLVLDGDATHRPSKNIV
                     ARVERGDRWIAISTPRSGWFGCVGERGTGTAAFLELTDWAVRRFPDVSVFAMNSGGHE
                     YFFAGSHRIVGQAPPPAATLVWAHIGATLAARDAREANGRLVMLDTADPQRSLMATDA
                     AREAAAAAFSGLSGLTTPGRVQPNAGELSTFTDRGYATAFAVLGVHSWFHTVEDTLER
                     VDASLVTPVVAAHQRAIELLV"
     gene            1419..1799
                     /locus_tag="AC16CY_02445"
     CDS             1419..1799
                     /locus_tag="AC16CY_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3352665.1"
                     /translation="MNIALWILQVLLAVVFVLHGWLFLSPPPAIAAQMNESLPRWFQL
                     FLGVAEVLAAVGLILPGLTRILPWLVVWAAGGIMVVLASATVWHVVRDEMSSAAITLG
                     LLVMATVVAYLRRRVAPIHSRGPA"
     gene            complement(1939..2091)
                     /locus_tag="AC16CY_02450"
     CDS             complement(1939..2091)
                     /locus_tag="AC16CY_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352666.1"
                     /translation="MSSFRGVTDGGVSGAALDRDEVRRLYQQHRRALLAYAARFVRPR
                     RGSPTR"
     gene            2304..3299
                     /locus_tag="AC16CY_02455"
     CDS             2304..3299
                     /locus_tag="AC16CY_02455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3352667.1"
                     /translation="MRRAGLLTAIVVAGLTVAGIAAQQRGGGRGAGPRIPPTGTIEKV
                     RDNLYKIAGAGSNTTVFVTSDGVVLVDTKLANNGEAILKQVQTVTDKPVSMIINTHTH
                     PDHNGSNDYFKAARPAVQVVAHENTKKWVAANPKADPAMTPDRTYSERLRLGTGKDRI
                     DLYYFGAGHTNGDAFVVFPEVRAMCIGDLMAWNMAPLIDPATGGSVIALPDTLEKALK
                     GIGNVDLVIEGHGNVNTWAGFRAYSEFNRELLTVAKAALSRGETPEQALATLAKNPKL
                     AVFLTEELLKDLEYGGTPKSRALINLNVAFQELKGEPVTTNFGPARGAAPPGAPK"
     gene            3344..4378
                     /locus_tag="AC16CY_02460"
     CDS             3344..4378
                     /locus_tag="AC16CY_02460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3352668.1"
                     /translation="MKRISCLPALLVATVAIVSADATDDFVRAEMKRQNIPGLSLAVV
                     KDGVVIKAAGYGVADLKTKAPATPETVYKIASVSKQFIATGIMLLVQEGRLGVDDAVG
                     KFVPGVPAAWNRITIRHLLTHTAGLVREPPAFSPFRVDTDASLIESAYATPLRFAPGD
                     RWEYSNLGYVILAEVIARVSGRPWSDFMTEKVFQPSGMAATRTTTTAPVVNKAAGYSN
                     NDKLIDAPDWPAVRAGGAFLSTVLDLARWDAVLRANAILTESSRRQTWTPARLNDGAP
                     YAYGFGWHINRPGARRQVWHSGGLPGFQAQYRRYLDEGVTVIILMNLDDADDEGIAAG
                     VAERHLSDRP"
     gene            4460..4942
                     /locus_tag="AC16CY_02465"
     CDS             4460..4942
                     /locus_tag="AC16CY_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352669.1"
                     /translation="MKAHTRLIPIASVLLCMAGVPAAAQTDTAKDAAKKTGTVVAEGA
                     KTTGSAVSDAAKATAKGTTDVAKKTATATAKGAEKTATAATKGAETVAKPVGDASKAT
                     AKKTTEVAKKTGSTTADAAKKSGETAAKGAEKTASGAKKLGLGIRDAVTPDEKKSDKK
                     "
     gene            5014..>5257
                     /locus_tag="AC16CY_02470"
     CDS             5014..>5257
                     /locus_tag="AC16CY_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352670.1"
                     /translation="MRRLMLGALTVLAVVAIVTTSGVSGQGKVDSPGVMTPDGNVPAL
                     GSFKVPKTPWGEPDLQGTWNANDLQGVPMQRAESVGT"
BASE COUNT          956 a         1702 c         1800 g          799 t
ORIGIN      
        1 agacgagtaa ctcgatcgct cgctggtgcg cagccacgac cggcgtgacg agcgaggcgt
       61 ccacgcgctc gagcgtgtct tccacggtgt ggaaccagga atggacgccg agcaccgcga
      121 atgccgtggc atagccgcgg tcggtgaacg ttgacaactc gcccgcattc ggctgaacac
      181 gacccggcgt cgtcaggccg ctgaggccgc tgaaggcggc ggccgcggcc tcgcgggccg
      241 cgtcggtcgc catcaggctc cgttgcgggt cggcggtgtc gagcatgacg agacggccat
      301 tcgcctcccg cgcgtcgcgc gcggcgagcg tggcgccgat gtgcgcccag accagcgtgg
      361 cggccggagg cggcgcctga cccacgatgc ggtgcgagcc ggcgaagaag tactcgtgtc
      421 cgccgctgtt catcgcgaag acggacacgt ccggaaaccg gcgcacggcc caatcggtca
      481 gctccaggaa cgcggccgtg cccgttccgc gctcgccgac gcatccgaac caccctgaac
      541 gaggagtcga aatcgcgatc cagcgatcgc cacgctcgac tctggcgacg atgttcttgc
      601 tgggacggtg agtggcgtcg ccgtccagca cgagcgtggc ctgcgatcgc gatcgggcgg
      661 cgtcgacgag cgccgtggcg cgccgcggtg cgatgacagc agccggaatc ggcacgaacg
      721 gttcctcggg gctgttgagc gccacggcct cgccactcgg accagtcgtc accatgacga
      781 tggcgcgcgc tccagccgcg gccacctgct tgacggtctg tccgataccg cgatcgggaa
      841 agagcgcggc atggcggccg aaggggagga ccacaatcgc gagccggttc ttcacgtcgc
      901 cgacggcgcc gccttcgacg agcgcgagcg gcgccgtgac gccggccggc ccggtcggga
      961 tgacgggcgc ctgcggcacg acgtcaactg acgtggttcc gctggtgagc cgcgccgtgc
     1021 gcttgacgaa gaagggcgcc tggaactccg actcctcgac tcgatatccc gattgccgaa
     1081 gccggccggc gatccaacgc gccgtcgcga ggtcacccgg tccgcccgag aatttgtcgc
     1141 cgaaggccgc gtgctgagcg aggtgcgcgc ggatacggtc cgtcattgcc tcgaccccag
     1201 gctgcgcgaa ggccggcctg gcaaccaggc ctggtaccat cgtcgcggcg gccccacagg
     1261 cgagcctgct caacaactct cggcgtgtgc tctgcatctc cgctggaact ccgtcaactc
     1321 ccaactccca acacctccgc ttgggaattg ggaattggga gttgagattg cgcggccaag
     1381 cataccagtc cgccgtgctg gcggtattct gtcctctcat gaacatcgcg ctttggatcc
     1441 tgcaggtact gctcgcggtc gtgttcgtcc tgcatggctg gctgtttcta tcgccgcccc
     1501 cggcgatcgc cgcccagatg aacgagtcgc tgccgagatg gtttcagctc tttctcggcg
     1561 tagcggaagt cctggcggca gtggggctca tccttcctgg cctgacacga atcctgccct
     1621 ggctcgtcgt atgggccgcc ggcggaatca tggtcgtact ggcgtcggcg acggtgtggc
     1681 acgtggtacg cgacgagatg agctcggcgg cgatcacgtt ggggctgctg gtgatggcaa
     1741 cggttgtggc ctatctgcgc cgtcgcgtcg cgccaatcca ctcacgcggc ccagcctaac
     1801 gagcgagcgc gtcgagcacc ttctgacgcg cggcgcggat cgcctcccgc tggtcgttga
     1861 tcctgggtgg agacggcaac ggaaacgaac cgacgttcac gcccgcgagc gctgcacgcg
     1921 ctttctctgg aggcaggctc agcgcgtcgg cgatccgcga cgcggccgta cgaagcgcgc
     1981 cgcgtaggca agcagggccc ggcgatgctg ttggtacagc cgccgcacct cgtcccggtc
     2041 gagcgccgcg ccactcacgc cgccatccgt cacgcctcga aacgatgaca cgcgagcggc
     2101 tcgtttctgt ctaatcgcat cgttcgcctg tcttgacact ggaatacgtc acaccaaggc
     2161 acagaggcac cgagacactg tgggaattcg tctcggtgtc tcggagcctc ggtgtgtcgt
     2221 gatcgtcaac tgaaacgagt tccgagtcgc gatgaaacga gagcgcgggt catttgcggg
     2281 tattctggtc cgggaggaac ccgatgagac gtgccggact tctgacagca atcgtggtgg
     2341 ctggcctgac cgtggccggc atcgcggcgc agcagcgcgg aggcggacga ggcgccgggc
     2401 cgcggattcc gccgaccggc acgatagaga aagtccggga caacctctac aagatcgccg
     2461 gcgccggcag caataccacg gtgttcgtca cgtctgatgg cgttgtcctc gtggacacca
     2521 agctcgccaa caacggtgaa gcgatcctga agcaggtgca gacggtcacc gacaaaccgg
     2581 tgtcgatgat catcaacacg cacacgcacc cggaccacaa cggcagcaac gactacttca
     2641 aggccgccag gccggcagtc caggtcgtcg ctcacgagaa cacgaagaag tgggtcgccg
     2701 ccaatccaaa agccgacccg gcgatgacgc ccgaccgcac ctattcggag aggctgaggc
     2761 tggggacggg caaggaccgc atcgacctct attacttcgg cgccggccac accaacggtg
     2821 atgccttcgt ggtgttcccg gaggtccggg ccatgtgcat cggcgacctc atggcatgga
     2881 acatggcgcc gctgatcgat ccggccaccg gcggcagcgt catcgccctg ccggatacgc
     2941 tcgagaaggc gctgaagggc atagggaacg tggacctcgt catcgagggg cacgggaacg
     3001 tgaacacgtg ggcgggcttc cgcgcctaca gcgaattcaa tcgcgagctg ctgacggtgg
     3061 cgaaggcagc cttgtcgcgc ggcgagacgc cggagcaggc gctggccacg ctcgcgaaga
     3121 acccgaagct cgccgtgttc ctgacggaag aactgttgaa ggacctcgaa tacggcggca
     3181 cgccgaagtc gcgagcgctc atcaacctga acgtcgcctt tcaggagctg aaaggcgagc
     3241 cggtgacgac gaatttcggt cccgcccggg gcgcggcccc gccgggggcg ccgaagtaga
     3301 ggcgggtctt ccctggcttg agcggagtcg aaggcagacg cgcatgaaac ggatctcttg
     3361 tcttcccgcg ctgctggtcg cgaccgtcgc gatcgtctcg gcggacgcga ccgacgactt
     3421 cgtccgcgcc gagatgaagc gccagaacat ccccggactg tcgctggcgg tcgtcaagga
     3481 tggcgtggtg atcaaagccg cggggtacgg cgttgcagac ctcaagacga aggccccggc
     3541 cacgcccgag acggtctaca agattgcgtc cgtcagcaag cagttcatcg cgaccggcat
     3601 catgctgctc gtgcaggaag gccggctcgg cgtggacgat gcagttggaa agttcgtccc
     3661 cggagttccg gctgcgtgga accggatcac cattcggcat ctgctcacgc ataccgccgg
     3721 gctcgtgcgc gagccgccag ctttcagccc gttcagagtc gacaccgatg ccagcctgat
     3781 cgagtcggca tacgcaacgc ccctccggtt cgcgccaggg gaccggtggg agtactcgaa
     3841 tctcggttac gtgatcctgg ccgaggtcat cgctcgggtg tccggcaggc cgtggagcga
     3901 cttcatgacg gaaaaggtgt tccagccttc cggcatggct gcgacccgca cgacgacgac
     3961 ggcgccggtc gtcaacaagg ctgcggggta ctctaacaat gacaagctga ttgatgcccc
     4021 ggactggccg gccgttcgag ccggcggagc gttcctgtca acggtactgg acctcgccag
     4081 gtgggacgcc gtgctccgcg ccaatgccat cctgaccgag tcgtcccgca ggcagacgtg
     4141 gacgcccgcg aggctcaacg acggagcacc gtacgcctac ggcttcggtt ggcacatcaa
     4201 tcggcctggc gcgcggcggc aggtctggca cagcggcggc ctgccggggt tccaggcgca
     4261 gtaccgccgc tacctggacg agggcgtgac ggtcatcatc ctgatgaacc tggatgacgc
     4321 cgacgacgag ggtatcgcgg ctggcgtagc cgagcgccat ctctccgatc gcccgtagcg
     4381 cacgggcgcc cggctggcgg cggcacgcga atggtggttc aatctaagga gctcaagtcg
     4441 agcaacggag agagcgatca tgaaggcaca cacacgtttg attccaatcg cctcggtgct
     4501 cctgtgcatg gctggtgttc ctgctgccgc gcaaaccgac acggcgaaag acgccgcgaa
     4561 gaagacaggg actgtcgtgg ccgagggggc caagacgacc gggtccgcgg tgagtgacgc
     4621 ggcgaaggcc actgccaagg ggaccactga cgtcgccaag aagaccgcca ccgctaccgc
     4681 caagggcgcc gagaagaccg cgaccgccgc caccaagggc gccgagaccg tggcgaagcc
     4741 ggttggagat gcgtccaagg cgacggcgaa gaagacgact gaggtcgcca agaagaccgg
     4801 cagcaccacc gccgatgccg cgaagaagtc cggggagaca gcagccaagg gcgccgagaa
     4861 gacggccagc ggcgcgaaga agctcggtct cggaatcagg gacgcggtga cgccggacga
     4921 gaagaagtcg gacaagaagt aggcgcggta gctgctgtac actcttacga acgcagggcg
     4981 gccgtcgcgt acgaagccgc cgggggagag cacatgagac gcctcatgct tggagcgctg
     5041 acggtgttgg ccgtcgtcgc gatcgtcacg accagcggtg tgtccggaca gggcaaggtc
     5101 gattcgcctg gggtcatgac gccggatggg aacgtgccgg cgctcggcag cttcaaggtg
     5161 ccgaagacgc cgtggggcga accggatctc cagggcacct ggaacgctaa cgacctccag
     5221 ggcgtcccga tgcagcgcgc cgagagcgtc ggcacgc
//
LOCUS       DBNGSH010000112         4028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_517234, whole genome shotgun sequence.
ACCESSION   DBNGSH010000112 DBNGSH010000000
VERSION     DBNGSH010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_517234"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2887)
                     /locus_tag="AC16CY_02475"
     CDS             complement(<1..2887)
                     /locus_tag="AC16CY_02475"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3352671.1"
                     /translation="MARRITAFLLGFVFMFACTLDAQERFGGLTGTVTDVSDAAVPGA
                     TVTATNKATSAARVAVSGGDGVYRVPDLEPGRYSVTIELQGFQTVALDDVIVLLGKTV
                     SLDAQLRPGAVSEIVKVTADATRQLDLTSVTLAHNVTSEELDRLPKSRSYQGIALVAP
                     GVNAGEIEGGIQVHGASSAENVFTIDGVATSSLIYGYSRQNTVFEYLQEVQVKTGGID
                     AEYGGALGGVISAVTKSGGNLFTGEAHYYYLGSALGGAPVKRLVLDPNDLTTVGYFQD
                     TEQPNAQNEFGGSIGGPIVTNRVFFFGSVSPRVVRRTNEYLFSAGTEPGTVAQDQTAW
                     QAFGKVTYASGRFHANASLLMSPTRSTGRLPAYDSTGTNFLITSSEANEANRTRGFEN
                     DQNNVGGNLDVTLNNSAYLSARGGYFYDSYKDTGVPLTTSYTYQTPNFTVPGVPPSLQ
                     GPQGTANTPRIQISNFDKTTQGFIQLDYNHSFKAAGSHLLKGGWGVRRSMNDVDVSYP
                     GGYVYLFWGQSFRSGVTGVTDTGTYGYYYVSDLATRGAVHANIQTLFVQDTWNVGPRL
                     TLNLGLRTENEKIPTFQPEIAQYAFQFGFADKLAPRLGAAYDVRGDGRTKAFASWGRY
                     YDWTKYEIARGSFGGDLWHLYYRSLDTLDIGSLSLDNMPGRDLWGSATGYRDLRGTAI
                     RNTDPDIKPMFQDSFNAGVDHQLNAATALGIHYVHNSLGRTIEDMGSIVNGSSLYVIG
                     NPGEGQNTITPPAHGATEPFATPKPERQYDALEVTLERRFSNNWFASANYTFSRLYGN
                     YSGTANSDEIQTPTTGVTSANAQAQLGSIARPGSNAHTGWDIDQVLWDSNGNLNLLGR
                     LATDRPHVVKLYGAYQFNFGTQLGAFWYGGSGTPLSTQVVGLDLYAPLVNGRGDMGRT
                     PVLTRTDLLISHELPMADRKRLRLELNIQNLFNQKTATHRYNFLNKGG"
     gene            3157..>4028
                     /locus_tag="AC16CY_02480"
     CDS             3157..>4028
                     /locus_tag="AC16CY_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HIR3352672.1"
                     /translation="MSVGRSAVPTALLGVVLVVLAGCSTGTRVDPAAVDVDAAMKETE
                     EWRARHEADYRRDWSTIAGLHFLEQGSYTAGSARGNDIVLPPSAPGVIGRFVLEGDVV
                     RFEPERGAKVLLGADPVTRSIPLADDGAPKADELHAGGIRMVVHRSGKRKSLRVWDPE
                     GELARGFLGFRWFPIQMTHRVTGRFIADAAPRDVQVVNTFGDLDNFKTEGVVEFTLQG
                     QTLRLRPFTTRPKRLYFVFKDASSGQETYEAARFLYSDLRDDGTTVLDFNQAYNPPCA
                     FNPFTTCPIPLPENR"
BASE COUNT          625 a         1271 c         1369 g          763 t
ORIGIN      
        1 gccctccctt gttcaggaag ttgtagcggt gcgtggccgt cttctggttg aacaggttct
       61 ggatgttcag ctcgagccgg agccgcttcc ggtccgccat cggcagctcg tgcgagatca
      121 ggaggtccgt ccgcgtcagc acaggcgtgc gacccatgtc gccgcgcccg ttgacgagcg
      181 gcgcgtacaa gtccagcccg acgacctgcg tgctgagtgg cgtgccgctg ccgccgtacc
      241 agaaggcgcc cagctgcgtg ccgaagttga actggtaggc gccgtacagc ttcaccacgt
      301 gggggcgatc cgtcgcgagt cgtcctagca ggttcaggtt gccgttcgag tcccagagca
      361 cttgatcgat gtcccatccg gtgtgcgcgt tgctgcccgg gcgggcgatc gagccgagct
      421 gcgcctgcgc gttggccgac gtgacgccgg tcgtgggagt ctgaatctcg tccgagttgg
      481 ccgtgccgga gtaattcccg tacagccggc tgaacgtgta gttggcgctg gcgaaccagt
      541 tgttcgagaa gcgccgctcc agggtgactt cgagcgcgtc gtactgccgc tcgggcttcg
      601 gcgtcgcaaa cggctccgtg gcgccgtggg cgggcggagt aatcgtgttc tgcccctcac
      661 cgggattgcc gatcacgtag aggctgctgc cgttcacgat cgatcccatg tcctcgatcg
      721 tgcggccgag cgagttgtgc acgtagtgga tgcccagtgc cgtagcggcg ttcagctggt
      781 ggtccacgcc ggcgttgaag ctgtcctgga acatcggctt gatgtcggga tcggtgttcc
      841 ggatggccgt gccgcgcagg tcgcggtagc ccgtcgcgct gccccagaga tcgcggccgg
      901 gcatgttgtc cagactgagg ctgccgatgt cgagcgtgtc cagcgagcga tagtagaggt
      961 gccagaggtc gccgccgaac gatccgcgcg cgatctcgta cttggtccag tcgtagtacc
     1021 gcccccagct ggcgaacgcc ttcgtccggc catcgccccg cacgtcgtag gccgccccca
     1081 gccgcggcgc cagcttgtcc gcgaacccga actgaaacgc gtattgcgcg atctcgggct
     1141 ggaaggtcgg gatcttctcg ttctccgtgc gcagcccgag gttcagcgtc agccgcggtc
     1201 cgacgttcca ggtgtcctgc acgaagagcg tctggatgtt ggcgtgcacc gcgccgcgcg
     1261 tcgcgaggtc gctcacgtag tagtagccgt aggttcccgt gtcggtgacg ccggtcacgc
     1321 cgcttcggaa cgactgtccc cagaacaggt acacgtagcc gcccgggtac gacacgtcca
     1381 cgtcattcat cgatcgccgc acgccccatc cgcctttcag cagatgcgag ccggccgcct
     1441 tgaacgagtg gttgtaatcg agctggatga acccctgcgt cgtcttgtcg aagttgctga
     1501 tctggatgcg cggagtgttg gccgtcccct gcgggccctg aaggctcggc ggaacgcccg
     1561 gcacggtgaa gttcggcgtc tgataggtgt agctggtcgt cagcggcacg cccgtgtcct
     1621 tgtagctgtc gtagaagtac ccgccgcgcg cgctgagata cgcgctgttg ttcaacgtca
     1681 cgtcgaggtt gccgccgacg ttgttctgat cgttctcgaa cccgcgcgta cggttcgctt
     1741 cgttcgcctc tgaggacgtg atcaggaagt tcgtgcccgt tgaatcgtag gcgggcagcc
     1801 gccccgttga ccgtgtcggc gacataagca ggctggcgtt ggcatggaac cgcccggagg
     1861 cgtaggtcac cttgccgaag gcctgccacg ccgtctggtc ttgagccacg gtgccgggtt
     1921 cggtccccgc cgagaacagg tactcgttgg tccggcgtac gacacgagga gagaccgagc
     1981 caaagaagaa gacacggttg gtcacgatcg ggccgccgat ggatccgccg aattcgttct
     2041 gggcgttcgg ctgctcggtg tcctggaagt agccgaccgt ggtgaggtcg ttcgggtcga
     2101 gcacgagccg cttgaccggg gcgccgccaa gcgcgctgcc gaggtagtag taatgggcct
     2161 cgccggtgaa gaggttgccg ccagatttcg tcaccgcgct gatcacgccg ccgagcgcgc
     2221 cgccatactc ggcgtcgatg cccccggtct tcacctgcac ttcctgcagg tactcgaaca
     2281 cggtgttctg acgtgagtac ccgtagatca ggctgctcgt cgcgacgccg tcgatcgtga
     2341 agacgttctc ggcgctgctg gcgccgtgaa cctggatgcc gccctcgatc tcgccggcgt
     2401 tcacgccggg cgcgacgagc gcgatcccct ggtaactcct gctcttcggc aggcggtcca
     2461 gctcttcgga ggtcacgttg tgcgcgagcg tgacgctggt cagatccagc tgccgcgtcg
     2521 cgtccgccgt caccttgacg atctcgctga ccgctcccgg ccgtaactgc gcgtcgagac
     2581 tcaccgtctt gcccagcagg acgatgacgt cgtcgagcgc gacggtctgg aacccctgca
     2641 gctcgatcgt gaccgagtag cggccgggct cgagatccgg cacgcggtag acgccgtcgc
     2701 caccgctgac tgcgactcgc gcggcgctgg tcgccttgtt ggtggccgtg acggtcgcac
     2761 ccggcacagc tgcgtcgctg acgtcggtga ccgtgccggt caagccgccg aatcgttcct
     2821 gcgcgtcgag cgtgcaggca aacatgaaga caaaccccag aagaaacgcg gtgatgcgcc
     2881 tcgccatcgt ccactctcct cacttggcgt gtgcccgcgc catgttgaac gggttacgcc
     2941 ggacgccttt gataggagag aaccgtgccg ttgggacgag tgacgagtga cgagctgtcg
     3001 gggacgaggt gcgtctgcca gggggtaacg gatccgcggg tgtcggctcc gtacagacta
     3061 ttggacagga ttcagaaatc tgaatctcga gagcagccgt tcgaccctcg aattcgcctc
     3121 ccggccacaa cagcgataag ctagccgcgc gtggccatga gtgtgggtcg ttcggctgtt
     3181 ccgacggcgt tgctgggcgt cgtccttgtc gtgctggcag gctgcagcac agggactcgt
     3241 gtcgatcctg ccgcagtgga cgtggatgcc gcgatgaaag agacggaaga gtggcgggcc
     3301 aggcacgagg ccgactatcg gcgcgactgg tccacgattg ccggcctgca cttcctggag
     3361 cagggcagtt acaccgcggg cagcgcgcgc ggcaacgaca tcgtccttcc cccgtcggct
     3421 cccggtgtca tcggccggtt cgttctggaa ggcgatgtcg tgcggttcga gcccgagcgc
     3481 ggggcgaaag tgcttctcgg ggcagacccg gtgacacggt ccatcccgct tgccgacgac
     3541 ggggcgccga aggctgacga gctgcatgcg ggcggcattc gcatggtcgt gcaccggagc
     3601 ggaaagagaa agtcactccg tgtgtgggat cccgagggtg agctggcgcg cgggtttctt
     3661 ggcttcaggt ggttcccgat tcagatgacg caccgcgtca ccggccggtt cattgcggat
     3721 gcggcgccgc gagacgtgca ggtggtgaac acgttcggcg atctcgacaa cttcaagacc
     3781 gagggtgtcg tcgagttcac gctgcaaggg cagacgctcc ggctgcggcc gttcacgaca
     3841 cggcccaagc ggctgtactt cgtgttcaag gacgcgtcga gcggccagga gacctacgag
     3901 gcggcgcgtt ttctgtactc ggaccttcgc gacgacggga ccacggtgct cgatttcaac
     3961 caggcgtaca acccgccgtg cgcgttcaac cctttcacga cgtgcccgat tccgctgccg
     4021 gagaaccg
//
LOCUS       DBNGSH010000113         4259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1365998, whole genome shotgun sequence.
ACCESSION   DBNGSH010000113 DBNGSH010000000
VERSION     DBNGSH010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1365998"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="AC16CY_02485"
     CDS             complement(<1..1285)
                     /locus_tag="AC16CY_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3352673.1"
                     /translation="MDARVTTASTSAAPDVAWYRTVTREQWRVLLAAKFGWMLDAMDF
                     MLYTMAVGELRTYFGFGDDMAGFLGTATLVMSGVGGAIFGYVADRFGRTRALMGTILI
                     FSFASLGAATSQTVLQLLFWRSVLGIGMGGEWASGAVLVSETWPAALRNKAISIMQSG
                     WALGYMMASISAAVILGAPSLGADAWRWLFVLGVVPAFFTLWIRRYVREPESWSRRRQ
                     TLGRASPFAIFGPALIRRTLLIIALGSAVQFANWGLFFWLPQFLSRPVAQGGAGMGIV
                     GSLPWIIPVQLGAYAGYLTFGFIADRIGRRQAFVLYMIAAALLVPVYGQMARSPVVLL
                     LLGPLLGYFGYGYFSMFGGFVAELYPAAVRATGQGTSYNIGRMAGAVAPFTIGVIATQ
                     PGIGIGLALSVTSAFFLLAAALVFTLPDRSGQPLDE"
     gene            1401..1868
                     /locus_tag="AC16CY_02490"
     CDS             1401..1868
                     /locus_tag="AC16CY_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3352674.1"
                     /translation="MSVDVGAKAPDFTLPNQDREPVTLSAQLSKGPVVLAFMPAAFSG
                     TCTAEMCRFRDSASELNKVKATVLGITVDTFFALKAWADAQRLTFPLLSDFNKDIIRK
                     YGVVNPDMIGLKDISKRAVFVIDRSGVVRHREVLDDARNEPDYAKVTQALSSL"
     gene            1961..3310
                     /gene="dnaB"
                     /locus_tag="AC16CY_02495"
     CDS             1961..3310
                     /gene="dnaB"
                     /locus_tag="AC16CY_02495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR3352675.1"
                     /translation="MAETAVATAERTLPHNLEAERSVLGAILVHNDAFNLAAQSIDSD
                     DFYRDAHRRIFDRMVALNERHEAIDFVTLKEELARAGELDEVGGPAYVASLADGVPRA
                     TNVEYYARIVKEKATLRNLIYAANKILTNAYEADQDADLILDEAEKEIFSVADDRVKA
                     GFVSMRELVKDSFPKIEQLFEQKKLITGVPTGFVDLDELTRGLQAGDLVIVAARPSMG
                     KTSLVLNISQYVAGLSGHAVGLFSLEMSKEALFLRLLTSEAQIDGQRLMAGAIGGNDY
                     HRISHALEKLNAMKLYIDDTANIGVLEMRAKARRLQNEHGLSLLIVDYLQLMNARGRF
                     ENRTLELASISRSLKALAKELKVPVIALSQLSRAPEARSDHRPQLSDLRESGALEQDA
                     DVVVLIYRDDVYNKDPNSPDAGTAELILAKQRNGPTGTVRLAFLREQTRFANLAQGA"
     gene            3316..>4259
                     /gene="alr"
                     /locus_tag="AC16CY_02500"
     CDS             3316..>4259
                     /gene="alr"
                     /locus_tag="AC16CY_02500"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3352676.1"
                     /translation="MVRSTVARVDLNALKSNFAAIRTFLSRSAEASASAASSRPPAII
                     AVVKANAYGHGAPRVGLALEEAGATMLACADIEEGIVLRQAGVRVPILVFGALSVSDL
                     EGLFVCALTPTISTPGAARAVQAAAARHRSTIAYHLKIDTGMNRLGFRHDNLRRTLLE
                     LLASPNLRLDAVYTHFASADDPESPIFNTQRERFEAAWKVVQDLSPISDPQSRVLRHA
                     CNSAALLRDSRVWYDAVRPGLLLYGIVPPPLAATIDLRPVMSLTSRVVAVKGLRPGEG
                     VGYGWRYETREPRSIAVVPAGYADGLDTRLCGRGQVLV"
BASE COUNT          786 a         1447 c         1388 g          638 t
ORIGIN      
        1 tctcgtcgag gggttgaccg ctccggtcgg gaagcgtgaa gacgagcgcc gcagccagca
       61 ggaagaaggc tgacgtcacg ctcagtgcga ggccgatgcc gatgcccggc tgtgtcgcaa
      121 tcacgccgat ggtaaagggc gccacggcgc ccgccatccg gccgatgttg tagctcgtgc
      181 cctggcccgt ggcgcgtacc gcggcgggat acagctcggc gacgaagccg ccgaacatgc
      241 tgaagtagcc gtacccgaag tagccgagca gcgggccgag cagcagcagg acgaccggac
      301 tgcgcgccat ctgtccgtac accggcacga gcagggcagc cgcgatcatg tacagcacga
      361 acgcctgccg ccgcccgatg cgatcggcga tgaagccgaa cgtgaggtag cccgcatacg
      421 cgcccaactg caccggaatg atccagggaa gcgatccgac gatgcccatg cccgccccgc
      481 cttgcgccac ggggcgagac agaaactgcg gcagccagaa gaagagtccc cagttggcga
      541 actgcactgc cgagccgagc gcgatgatga gcagcgtccg ccggatcaag gccggcccga
      601 agatggcgaa cggactcgcc ctgcccaacg tctgccgcct gcgggaccag ctctcgggct
      661 cgcggacgta gcggcggatc cacagggtga agaacgcggg gacgacgccc agcacgaaga
      721 gccaccgcca cgcgtcggcg ccaagcgacg gcgccccgag gatcacggca gcggatatcg
      781 aggccatcat gtacccgagg gcccaccccg actgcatgat gctgatcgcc ttgttccgga
      841 gcgcggccgg ccacgtctcg ctcacgagca cggcgcccga cgcccactcg ccgcccatgc
      901 cgataccgag cacggagcgc cagaagagca gctgcaggac cgtctgcgat gtcgcggcgc
      961 cgagcgaggc gaaggagaag atcaggatcg tccccatcag cgcccgcgtg cggccgaatc
     1021 ggtcggccac gtagccgaaa atcgcgccgc cgacgcccga catcacgagc gtcgccgttc
     1081 cgagaaaacc ggccatgtcg tcgccgaagc cgaagtacgt gcggagctcg ccgaccgcca
     1141 tggtgtagag catgaaatcc atggcgtcga gcatccagcc gaacttggcg gccagcagca
     1201 cgcgccactg ctcgcgcgtg accgtgcggt accacgccac gtcgggcgcc gcagacgtcg
     1261 aggctgtcgt gacccgtgcg tccatcgaag cccgtagacc gtagaccgta gtgcgtcgtg
     1321 cggtcaagcg tgggaaaccg ctacaatgcc cacttcaccg ccgttgtcgt cgtccgggcg
     1381 tgcccggaaa ggagatcgga atgtccgtag atgttggagc caaggcgcca gacttcaccc
     1441 tcccgaacca ggatcgagag ccggtcacgt tgagcgcaca gttgtcgaag ggtcccgtgg
     1501 tgctcgcgtt catgcccgcg gcgttcagcg gcacgtgcac ggccgagatg tgccggtttc
     1561 gtgattcggc gtccgagctg aacaaggtca aggccaccgt gctcggcatc accgtcgaca
     1621 cgttcttcgc gctcaaggcg tgggcggacg cgcagaggct gacctttccg cttctcagcg
     1681 atttcaacaa ggacatcatc cggaagtacg gcgtagtgaa cccggacatg atcggcctca
     1741 aggacatctc gaagcgcgcg gtgttcgtga tcgatcggag cggcgtcgtg cgtcaccgcg
     1801 aggtgctcga cgacgcgcgc aacgagccgg actacgcgaa agtcacacaa gcgctgagca
     1861 gcttgtagcc acggcctgcg gtcttcggtc ttcagaccga agaccgtctc gtcaagattc
     1921 tgttacactc ctcgttcgcc tccgaaagac ccgcccgttc atggccgaga ctgctgtcgc
     1981 caccgcggag cgcacgcttc cgcacaacct cgaggccgag cgctccgtgc tcggcgcgat
     2041 tctggtccac aacgacgcat tcaacctggc ggcgcagtcg atcgacagcg acgacttcta
     2101 ccgcgacgcg caccgccgca tcttcgaccg catggtcgct ctgaacgagc gtcacgaggc
     2161 gatcgatttc gtcacgctga aggaggagct cgcgcgggcg ggcgagctgg acgaggtggg
     2221 cggtccggcc tacgtggcct cgctggccga cggcgtgccg cgcgcgacca acgtcgaata
     2281 ctacgcgcgc atcgtcaagg aaaaggcgac gctgcgcaac ctgatctacg ccgccaacaa
     2341 gatcctgacc aacgcgtacg aggcggacca ggacgccgat ctcatcctcg acgaagccga
     2401 gaaagagatc ttctccgtcg ccgacgaccg ggtgaaggcc ggtttcgtgt cgatgcggga
     2461 gctggtcaag gacagctttc cgaagatcga gcagctgttc gagcagaaga agctcatcac
     2521 cggcgtgccg accgggttcg tcgatctcga cgagctgacg cgcggactgc aggccgggga
     2581 tctcgtgatc gtggccgccc ggccgtcgat ggggaagacg agcctggtcc tgaacatctc
     2641 ccagtacgtg gccggactct cggggcacgc cgtggggctg ttcagcctgg aaatgtccaa
     2701 ggaggcgctc tttctcaggc tgctcacctc cgaggcgcag atcgacgggc agcgtctcat
     2761 ggccggcgcc atcgggggca acgattacca ccggatttct cacgccctcg aaaagctgaa
     2821 cgcgatgaag ctgtacatcg acgacacggc gaacatcggc gtcctcgaga tgcgagccaa
     2881 ggcgcggcgg ctgcagaacg agcacggcct cagcctgctc atcgtggact acctccagct
     2941 catgaacgcc cgcggacgct tcgagaaccg gacgctggag ctcgcctcga tctcgcgctc
     3001 gctgaaggcg ctggccaagg agctgaaggt gcccgtcatc gcgctgtcgc agttgagccg
     3061 tgctcccgag gcgcgatcgg accatcgccc gcagctctcg gacctccgcg agtcgggagc
     3121 gctcgaacag gatgccgacg tcgtggtgct catctaccgg gacgatgtct acaacaagga
     3181 tccgaacagc ccagatgcag gcaccgccga gctgatcctc gccaagcagc gcaacggccc
     3241 gaccggcacg gtccgcctcg ccttcctgcg cgaacaaacc agattcgcca atctcgcaca
     3301 gggtgcgtag agcagttggt ccgtagcacc gtcgcccgcg tcgatctgaa tgcgctcaag
     3361 agcaacttcg cagcgatacg tacgtttctc tcccgtagcg cggaggcttc agcctccgcg
     3421 gcgtcgtccc gacctccagc gatcatcgcc gtcgtgaaag ccaatgcgta cgggcatggc
     3481 gcgccgcgcg tggggctggc gctcgaggag gccggcgcca cgatgctggc ctgcgcggac
     3541 atcgaagagg gcatcgtgct gcgccaggcc ggcgtgcgtg tcccgatcct cgtgttcggc
     3601 gcgctcagcg tgagcgatct cgaagggctc ttcgtgtgcg cgctgacgcc gacaatctcc
     3661 acccccggcg cggcccgcgc ggtccaggcc gcggcggcgc ggcaccgttc gaccatcgcg
     3721 tatcacctga agatcgacac cggcatgaac cggctcggct tccggcacga caacctgcgg
     3781 cgcacgctcc tggagctgct tgcgagcccg aacctgcgcc tcgacgcggt gtatacgcac
     3841 ttcgcgtcgg ctgacgatcc cgagagtccc atcttcaaca cacagcgcga acgcttcgag
     3901 gccgcctgga aggtcgtgca ggacctctcc ccgatctccg atccccaatc ccgagtcttg
     3961 cgccacgcct gcaacagcgc ggcgctgctg cgcgattcac gggtgtggta cgacgccgtc
     4021 cggccgggcc tcctgctgta cggcatcgtg ccgccgccgc tcgccgcgac gatcgatctg
     4081 cggcccgtga tgtcgctgac gagccgcgtg gtcgccgtca aagggctcag gcccggcgag
     4141 ggcgtgggct acggatggcg atacgagaca cgcgaaccgc gtagcatcgc ggtcgttccg
     4201 gccggctatg ccgacggcct cgatacgcgg ttgtgcggac gcggccaggt gctcgtcag
//
LOCUS       DBNGSH010000114         8435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1875588, whole genome shotgun sequence.
ACCESSION   DBNGSH010000114 DBNGSH010000000
VERSION     DBNGSH010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1875588"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..378
                     /locus_tag="AC16CY_02505"
     CDS             58..378
                     /locus_tag="AC16CY_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008186337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3352677.1"
                     /translation="MAKKTHVVGSDNVFADLGHPRPAEALAKAELARKIAGIIDGRGL
                     TQAAAAEILEVDQPKVSALIRGRLAGFSLDRLVRFLVLLGSDVEIVVKRRPKTARQAR
                     VLVA"
     gene            589..933
                     /locus_tag="AC16CY_02510"
     CDS             589..933
                     /locus_tag="AC16CY_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3352678.1"
                     /translation="MSDQERIQLLQGTLDLLVLQTLVFGPQHGHGIATYIQHTTEDEL
                     LVDHGSLYPAVQRLERAGLISSDWGTSDNNRRARYYKLTRAGRKRLVAETRKWEATVR
                     VISRVLNPRRAT"
     gene            930..3596
                     /locus_tag="AC16CY_02515"
     CDS             930..3596
                     /locus_tag="AC16CY_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3352679.1"
                     /translation="MKVPRWLRWRSNAELDEEIEAHLEIEVRANLDRGLSPDEARAAA
                     RRRFGNPTLVKQQAREADPLFQLETFLTDIRHTLRSLGRSPGFAVAAALTLAVAIGAT
                     TAIFSVVDGVLLRPLPYPDADRLVRVAGRTQPQVNVPNGELPFNTPSYWHFARNNRAF
                     AQFGGAEVYAGTNGVDEWTLIADGKPLPVDVARMTASAFELLGVNPLLGRLPTPEEDV
                     PGGPGLVLLSERLWRGLLGADPSFVGRSVELNGMRLDVIGVMPDDFGFPDPRVDVWLP
                     LQLNPASRDGRPSIHGIARLAPGATVESATADAEGLIARFDEIGFGGWTNTFTGEASV
                     RTLRDDLVGEVRRPLLILLGTMGFVLLIACSNVANLFQVRAEARALDTAVRAALGSGR
                     RRLVQLIVTEGLLLAAFGGAAGLALAWAGVRALVWMAPPGIPRLNAIGVNGTVLAFTA
                     GASIFAGLFFALLPALYTASTRMLRVLQAGGRSGAMGRKRHRARHVLVVTQVALALAL
                     FVASALMVRSFAALASVDPGFDPSGVLTFRLSPPPERYANPEAVARFYGKLLDGLRAL
                     PGVSSAGAAQFLPMTGGIGGLGGPFLGARIADAPPAPGESLPTFVFRRVAPGYFESMR
                     ISVIEGRTFTAEDDDLRRGSIVISRSVRDRYWPGGSAVGKRITIAGPPGTVVGVVEDV
                     RDTALDVAVEPVIYKPLLDATDGGMRMRAMTVTVRTTLDPFGLVPDVRRIVGSMDPEL
                     ALTDVESMASVVGRSLGRMRFTAVILALGAAVAFVLGAIGIYGVIAYGVSQRTGEIGV
                     RQALGADRTSVFTLILRDGLLLAGAGILFGLAASIGLGQVLSSLLYGVSPYDVVTLIS
                     GAAVFLAVAFFASAIPVLRAVRISPAVALRAE"
     gene            complement(3908..4330)
                     /gene="vapC"
                     /locus_tag="AC16CY_02520"
     CDS             complement(3908..4330)
                     /gene="vapC"
                     /locus_tag="AC16CY_02520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF047863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HIR3352680.1"
                     /translation="MPCYMLDTNTCSYIMKRSHPTVLRRLRAVPVRDVCMSVVTRAEL
                     LHGVEVSPRRAQDGAALAAFLPYVDAVDFGNDAALHYAEIRADLKRRGTMIGANDLFI
                     AAHARALGLTLVTNNTAEFGCVSKLAVENWTLAPRRKK"
     gene            complement(4330..4557)
                     /locus_tag="AC16CY_02525"
     CDS             complement(4330..4557)
                     /locus_tag="AC16CY_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIR3352681.1"
                     /translation="MPRTAKVFMTNRSQAVRLPKEYQFETDEVFIRKEGEDVILSPRP
                     RDWRSYLESAPIASEAFMADVEDLPVQEREA"
     gene            4750..5778
                     /locus_tag="AC16CY_02530"
     CDS             4750..5778
                     /locus_tag="AC16CY_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR3352682.1"
                     /translation="MTFTVTSNVKAAVLIALALAIGAMGIYVAEADDAPGGAVIALLL
                     MIGTVVLAVKAARNRLPAWAVRTALTVGVLIAALAAFMTHAVTVTAPPVAPSQGVPSV
                     IDSTPSPQYAGAVERARELVHAAVLEQNLPGVSVAVGIGLPSVASAKKGTIVWAEGFG
                     WRDIDTRTPVTPNTRFNIGTAASVVTAATVTSLGLTNTGAESAVEWSPEQIGEPGEDF
                     PPLALIRHLILQPLGLAPAEYPLPDDRATFYVPRSDDNPLSGRRLMYMRDLACCAGTM
                     ASYSTPSDLVRVGLANEGSINGELAGGMVMSLVTLRDSGIVVAVASNIAHANTSALGL
                     KIGDAFAR"
     gene            5922..6245
                     /locus_tag="AC16CY_02535"
     CDS             5922..6245
                     /locus_tag="AC16CY_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352683.1"
                     /translation="MSASRMQRLIEQPVAEGHVLEGGVPRGRVHYHLAVYQHFTGEDG
                     EPVPPNLEVEGRVIALDHFDVARFHRSGAELTLCLADGRLLDFRIVNDEGAIRSTARG
                     LYKAE"
     gene            6398..8155
                     /locus_tag="AC16CY_02540"
     CDS             6398..8155
                     /locus_tag="AC16CY_02540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3352684.1"
                     /translation="MKLSVIALDYDGTIARRDVLDSTVRPAIAAARTSGVTVLLVTGR
                     ILEDLQRVVGDLHLVDGVIAENGAVVHFPHSGHTSVLAPPVPEAFVVELRRRRIPFGL
                     GRSLVDADAALAPKLLEVIRALELPLVLVFNGGRVMVLPQGVSKATGLHAALDALRLS
                     ARNMVAIGDAENDHELLRLAEVGVAVEWASPALRSAADIVLQGSGPPAVAAYVRALAE
                     GGRLPIPPRARRRLILGHTEDGREFSLAVRGRNVLVAGDAKSGKSWVAGLLCEQLILH
                     GYCVCVIDPEGDYRSLEALPGVTVLGGEDRPPTPRDLLRALRYPDRSVVIDLSHRPQD
                     EKIEYIRAVLPALNEIRHRTGLPHRIVLDEAHYFLQDVGAGHLLDLERHGYAVVTYWA
                     SRLPRELLAATEVMIVTRESNPAEVEALRQWCSTCDKVDRSQWALLGQLRAGQAVALP
                     ITEEAGGELRLFTIAPRLTPHVRHREKYVDVPVAEHRAFIFSTNGHHSGQRARTLRQF
                     VSILEHAPVTLLEGHVRRGDFSRWIGDVFGDYPLATELRALEERHRAGASPDTLPEIV
                     AAIRGRYDLVRDQQARRAS"
     gene            complement(8253..>8435)
                     /locus_tag="AC16CY_02545"
     CDS             complement(8253..>8435)
                     /locus_tag="AC16CY_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352685.1"
                     /translation="GFTVMPGLGALIGLALDAVIPGKLRVVYQASPPAAASGASLMAV
                     PLVTSRAKGLAVVFAF"
BASE COUNT         1324 a         2748 c         2814 g         1549 t
ORIGIN      
        1 tgatcacgcg tcggttgaag caggccatcg cgatcagcga aggcagggag tagaacgatg
       61 gcaaagaaga cgcacgtcgt gggcagcgac aacgtgttcg cggacctggg acatccacgg
      121 ccggcggagg ccctggcgaa ggctgaactc gctcgcaaga tcgcaggcat catcgacgga
      181 cggggcctga cccaggccgc tgccgccgag atcctcgagg tcgatcagcc caaggtctct
      241 gcgctgatcc gcgggcggtt ggctggcttc tccttggacc ggctcgtgcg gttcctggtc
      301 ctgctaggaa gcgacgtcga gatcgtcgtg aaacggcgcc cgaagaccgc ccgtcaagct
      361 cgcgtcctgg tcgcataggc tccgcgtgct caatacgtgt ctccaccagc gcttttgagc
      421 ggatctagct gatcgtgccg aagcgagcag cccttaagtc gtttgttttc aacaagcggc
      481 agtggtggga atcccaccct ctccgccatt ctccagaacg gccagggacg ctccccagcg
      541 gtttccagta gactctctcc cagagactct ggtagggtct ctaccagaat gtctgaccag
      601 gaacgcattc aacttctcca gggcacgctc gacctgctcg tccttcaaac cttggttttc
      661 ggtccacagc acggccacgg catcgcgaca tacatccagc acacgactga ggatgaactg
      721 ttggtcgatc acgggtcgct gtatccggct gtccagcgac tcgaacgcgc cggcctcatt
      781 tcttccgatt ggggcacgtc agacaacaat cggcgcgccc gctactacaa gctcacccgt
      841 gccggccgca agcggctggt cgcagaaact cgcaagtggg aggcgaccgt tcgcgtcatc
      901 tcccgcgtgc tgaatccgcg gagggcgaca tgaaagtccc gagatggttg cgctggcgct
      961 cgaacgccga gttggacgag gaaatcgagg cgcatctcga gattgaggtt cgcgccaatc
     1021 ttgatcgcgg cctctcaccg gacgaagctc gagccgcggc gcggcgccgt tttggaaatc
     1081 ccacgctcgt caagcaacag gcccgcgagg ccgacccgct tttccagctc gaaacgttcc
     1141 tcaccgatat ccggcacacc ttgcgcagcc tcgggcgatc gccgggattt gccgttgccg
     1201 ccgccctcac gctcgcggtt gccatcggag cgacgaccgc catcttctcg gtcgttgacg
     1261 gcgtgctgct gaggccgctg ccgtatcccg acgccgaccg cctggtccgg gtggccgggc
     1321 gcacgcagcc ccaggtcaac gtcccgaacg gcgaactgcc cttcaacact cccagttact
     1381 ggcactttgc tcgcaacaac cgcgcgtttg cccagttcgg cggtgcggag gtgtatgccg
     1441 gaacgaatgg cgtcgatgag tggaccctga tcgccgacgg aaagccgctc cccgtcgacg
     1501 tcgcccggat gaccgccagc gcgttcgagt tgctcggcgt gaacccgttg ctcggccgat
     1561 tgcccacccc ggaggaagac gtgcccggcg gacccggcct cgtgctgctg agcgaacgcc
     1621 tgtggcgcgg tctgctcggc gccgacccgt ccttcgtcgg ccggtccgtc gagctcaatg
     1681 gcatgcgatt ggacgtcatt ggcgtcatgc cggacgactt cggctttccg gatcctcggg
     1741 tggacgtgtg gcttccgttg cagctgaatc ccgcgagccg ggacggcagg ccgagcatcc
     1801 acgggatcgc gcgcctggca ccgggtgcga ccgtcgagtc tgcgacagct gacgccgagg
     1861 gcctgatcgc gcgattcgac gagattggat tcggcgggtg gacaaacacc ttcaccggcg
     1921 aggcctcggt ccggacactg agagacgatc tcgtcggaga ggtccgccgc cccttgctga
     1981 ttctcctggg gacgatgggc ttcgttctgc tgatcgcctg cagcaacgtc gcgaatctgt
     2041 ttcaggtgcg ggcagaggcc cgggcgctcg acacggccgt tcgcgcagcg ctgggatcgg
     2101 gacggcggcg gctcgttcag ctcatcgtga ccgagggcct actgctggct gcgtttggcg
     2161 gggccgccgg gctcgcgctg gcctgggccg gcgtcagggc gctcgtgtgg atggcgccac
     2221 ccggtatccc cagactgaac gcgatcggcg tcaatggaac cgtgctggcg tttaccgccg
     2281 gcgcgtcgat cttcgcgggc ctgttcttcg cgctgctccc cgcgctctac acggcatcaa
     2341 cccggatgct gcgagtgctg caggcgggcg ggcgaagcgg ggccatgggt cgaaagcggc
     2401 accgggcccg gcatgtcctg gttgtcacgc aggtggctct ggcgctggcg ctcttcgtcg
     2461 cctcggcgct gatggtccgg agcttcgcag ccctggcatc ggtcgatccg ggcttcgatc
     2521 cctccggcgt gctgacgttt cgcctgtctc ctccccctga acgctacgcg aacccggaag
     2581 cggtcgcccg attctacggc aagctgctcg atggtcttcg tgcgctgccg ggagtctctt
     2641 ctgccggcgc tgcccagttc cttccgatga ccggcggcat cggcggcctc ggcggcccgt
     2701 tcctcggtgc gcgaatcgct gatgctccgc cggcgccggg cgaatcgctg ccaacgttcg
     2761 tgtttcgccg agtggcaccg ggctacttcg agtccatgag gatttccgtg atcgaggggc
     2821 gcaccttcac ggcggaggac gacgacctcc ggcggggatc gatcgtcatc agccggtcgg
     2881 ttcgggatcg ctactggccc ggtggcagcg cggtgggcaa gcgcatcacc attgccggcc
     2941 cgccggggac agtcgtgggt gtggtggagg acgttcgcga caccgcgctg gacgtggcgg
     3001 tcgagccggt gatctacaag ccgctgctcg acgcgaccga cggcggcatg cgcatgcgcg
     3061 ccatgacggt gaccgtgcgc acgacgctgg atccattcgg cctcgttccg gacgtccgac
     3121 gcatcgtggg gtcgatggat cccgaactgg cgctcaccga tgtcgaatcg atggcatccg
     3181 tcgtcggccg ctcgctcggt cgcatgaggt tcacggcggt gatcctcgct cttggcgccg
     3241 ccgtggcatt cgtgctgggg gcgatcggga tctatggggt gattgcgtac ggggtcagcc
     3301 agcgcacggg agaaatcgga gtgcgccaag cgcttggcgc cgaccgaacg tcagtattca
     3361 cgttgatcct gcgcgacggg ctgctcctgg caggagcggg gatccttttc ggcctcgcgg
     3421 cgtcgattgg attgggacag gtgctgtcgt cgctgctcta tggcgtgagt ccttacgacg
     3481 tcgtcacgct gatcagtggc gcggcagtgt tcctcgccgt ggcgttcttt gcgagcgcga
     3541 ttccggtctt gagagccgtg cgcatttcac cggcggtggc gttgcgggca gagtgaccga
     3601 agcggccgcc tgtaatcgcg caggacagcc gcatttgtct gtttcatcac ggcacctctc
     3661 caaatttggg ggcgatcatt gagtcgcgcc gaacgtaatg tcaagtacgg ttggtcgcaa
     3721 cacgtgatgg ccacggacgc ggtcgcgcaa tcgaggcagc cagtccactg tgcacaatcg
     3781 cctgatcagg acgtcgccat ccggcgggat tgagtcggag ccgattccga atctcgaaat
     3841 cagttcgcga acgcggcgcc agtctaagtc ctgcgtaacg tgcagtgtcg agtcagaagc
     3901 ccaggggcta cttcttgcgg cgcggggcga gggtccagtt ttcgaccgcg agtttgctca
     3961 cgcacccgaa ctcggccgtg ttgttcgtca ccagcgtcag tccgagtgcc cgcgcgtgtg
     4021 cggcgataaa gagatcattg gccccgatca tcgtgccccg ccgcttgaga tcggcgcgga
     4081 tctcggcata gtgcagcgcg gcgtcattgc cgaagtcgac agcatcgaca tacggcagga
     4141 atgcggcgag cgcagcgccg tcctgcgccc gtctcggcga gacttccacg ccgtggagca
     4201 gttccgccct ggtgaccacc gacatgcaca cgtctctgac gggaacagcc cggaggcgcc
     4261 ggagtaccgt cgggtgtgac cgcttcatga tgtaggagca cgtgttcgtg tccagcatgt
     4321 agcaaggcat tacgcctcgc gctcctgcac gggcaggtcc tccacatcgg ccatgaacgc
     4381 ttcggacgcg atcggcgccg attcgaggta cgagcgccag tcgcgcggcc ggggcgacag
     4441 gatgacgtcc tcaccctcct tgcggatgaa cacctcgtcc gtctcgaact gatactcctt
     4501 gggcaaccgg acagcctggc tgcggttggt catgaagacc ttggcggtgc ggggcatgat
     4561 gagcaaccct ccgcccccaa ttatgccata tacctcacgt atatggctct gcgtgctcga
     4621 tacgtatccc tcaccgccac cttccttcgc cgacccttag tcggaaatct gccgaaatca
     4681 ggcgaatctc acgagccgct cacgactcgg tgaggacttc gcggccggct ttcggtcaca
     4741 gtggtggcta tgacattcac tgtgacgagc aacgtcaagg cagccgtcct gatcgccctc
     4801 gccctggcaa tcggcgccat gggcatctac gtcgcggagg cggacgacgc gcccggcggc
     4861 gccgtcatcg cgctgctgct gatgatcggc acggtcgtgc tcgccgtgaa ggccgcccgg
     4921 aaccggctgc cggcctgggc ggtgcgcacc gcgctgaccg tcggcgtcct tattgcggcg
     4981 ttggcggcat ttatgaccca tgcggtcact gtgaccgcgc ctcctgtcgc gccgtcgcag
     5041 ggcgtgccat cggtcatcga ttcgacgccg tcgccgcagt atgcgggggc cgtcgagcgc
     5101 gcccgggagc tcgttcacgc ggccgtcctc gagcagaacc tgccaggggt ctcggtggcg
     5161 gtcggtatcg gcctgccctc agtagcctcg gcgaagaagg gcacgatcgt ctgggcggaa
     5221 ggattcggct ggagggacat tgacacgcgg acgccggtca cgccgaacac ccgattcaac
     5281 atcggcacgg cggcgtccgt ggtcactgcc gccactgtga cgtcgctcgg gctgaccaac
     5341 accggcgccg agtcggcggt ggagtggagt ccggagcaga tcggcgagcc gggagaggac
     5401 ttcccgcccc tcgcgctcat ccgccacctc atcctccagc cgctcggttt ggctcccgcc
     5461 gagtatccat tgccggacga tcgcgcgacg ttctacgtcc cgcgatctga tgacaatccg
     5521 ctaagtggaa ggcggctgat gtacatgcgc gacctggcgt gctgcgcggg gacgatggcg
     5581 tcctattcca cgccgtctga tctggtgcgc gtcggcctgg cgaacgaagg cagcatcaac
     5641 ggcgagctgg cgggcgggat ggtgatgtcg ctggtgacac tgcgcgacag cggcatcgtc
     5701 gtcgccgtgg cgtccaacat cgcgcacgcc aacacgtctg cgctcggcct gaagatcgga
     5761 gatgcgttcg cgagatgaga gccgcgctcg cggtcaccag ggcgccacgc gtcttccgac
     5821 ctgagccagc cagaaaggaa ggcgccgaag gccgaggaga gtagacttca gttcagctct
     5881 cggcagcagc tcaggagctc ctgtcacgtg gctggcgaga cgtgtctgcg agtcgcatgc
     5941 agcgcctgat cgagcaacca gtcgccgaag gccacgtgct tgaggggggc gtccctcgtg
     6001 ggcgcgtcca ctaccacctc gccgtatatc agcactttac cggcgaggac ggcgagcccg
     6061 ttccgccgaa tctcgaggtc gaaggtcgcg tgatcgccct cgaccatttc gatgtggccc
     6121 ggttccaccg cagcggcgcc gagttgacgc tttgcctggc ggatggccgc ctgctcgatt
     6181 ttcggatcgt caacgacgag ggcgcgattc gctccacggc gcgcggactg tacaaggcgg
     6241 agtgaatcca ctatcccgcc ccggctgtgc tgcggggggg tgcggaggat ttcgctgatc
     6301 cggctccctg ggcgagacgg tcgcccgcct tcaggcctga ttctgcgaca agtcggtggc
     6361 aggttgcttg ctccagcgac gagcgggagc ggacctggtg aaactgtccg tgatcgccct
     6421 cgattacgac ggcaccattg cgcgcagaga cgtgctggac tcgacggtgc gcccggccat
     6481 tgctgccgcc aggacgagcg gcgtgaccgt actgctggtc acgggacgga tcctcgagga
     6541 cctgcagcgg gtggtcgggg acctgcacct ggttgacgga gtcatcgccg agaacggcgc
     6601 ggtcgttcac ttccctcaca gcgggcacac gtcggtgctc gcaccgccgg ttccagaggc
     6661 gttcgttgtc gaactgcgcc gccgccgcat tcccttcggt ctcgggcgct cactggtgga
     6721 cgccgacgcc gcactcgctc caaagctgct cgaggtgatt cgagcgctcg aactgccgct
     6781 cgtgctggtc ttcaatggcg gtcgtgtgat ggtccttcct cagggcgtca gcaaggccac
     6841 cggtcttcac gccgcgctcg acgcgcttcg cctgtccgct cggaacatgg tcgcgattgg
     6901 tgatgcggag aacgaccacg agctgcttcg cctcgccgaa gtcggcgttg cggtggaatg
     6961 ggccagccct gcgttgcgct ccgccgccga catcgtcctc caggggtcgg ggccgccggc
     7021 cgttgcggcg tatgtgcgtg cgctggccga aggcgggcgc cttccgattc ctccacgggc
     7081 tcggcggcga ctgatactcg gccataccga ggatggccgc gagttttcgc tggcggttcg
     7141 gggccgcaac gtgctcgttg ccggcgatgc caagtcgggc aagtcgtggg tggccgggct
     7201 cctgtgcgag cagctcattc ttcacggcta ctgcgtgtgc gtcatcgacc cggaaggcga
     7261 ttaccggtcg ctcgaggctc tgccgggagt caccgtgctg ggcggcgagg atcgtccccc
     7321 gactccgcgg gatcttctgc gagcgctccg gtatccggat cgcagcgtcg tcatcgatct
     7381 atcccatcgg ccgcaagacg agaagatcga gtacatccgc gcggtcctgc cagccctgaa
     7441 cgagattcgt caccggactg gccttccgca tcggatcgtc ctggacgagg cccactactt
     7501 tctgcaggat gtcggcgcgg gtcatctcct cgatctcgag cgacatgggt atgcggttgt
     7561 gacctattgg gcgtctcgac ttccacggga gctcctggcc gccaccgaag tgatgatcgt
     7621 gacgcgtgaa tcgaatcccg cagaagtgga ggcgctgcgg caatggtgct cgacgtgcga
     7681 caaggtcgac cgctcgcagt gggcgctgct cggacagctg cgtgcgggcc aggccgtggc
     7741 gctgccgatt accgaggaag ccggcggcga gctgcgactg ttcacgatcg ctccgcgatt
     7801 gacgcctcac gtccgtcacc gggagaagta cgtggatgtc ccggtggcgg agcatcgagc
     7861 gttcatcttc tcgacgaacg gccaccactc cggccagcgc gcccgtacgc tgcgtcaatt
     7921 cgtgtccatc ctggaacacg cgccggtgac gctcctggag gggcacgtgc gaaggggcga
     7981 tttctcgcgt tggattggcg acgtcttcgg agactacccg cttgccaccg aattgcgagc
     8041 gctcgaagag cggcatcggg ccggcgcctc gccggacacc ctgccggaaa tcgttgcagc
     8101 gattcgcggg cggtacgatc tggttcgtga ccagcaggcc cggagagcca gctgaggctg
     8161 ttgcaccgca gtggggcgcg cagctctctc ccccctatgc cgcgtttgtt cccggtctgc
     8221 ggaagagcct caacggtgca tcagacactc gttcagaagg cgaacacgac cgcaaggcct
     8281 ttcgcgcgtg acgtcaccaa cggtacggcc atcaggctcg ccccggaggc cgcggcaggc
     8341 ggcgaggcct ggtagaccac gcgcagcttc ccgggaatca cggcgtcgag agcgagtcca
     8401 atcagcgccc caagccccgg catgacggtg aatcc
//
LOCUS       DBNGSH010000115         4227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4252885, whole genome shotgun sequence.
ACCESSION   DBNGSH010000115 DBNGSH010000000
VERSION     DBNGSH010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4252885"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..2580
                     /locus_tag="AC16CY_02550"
     CDS             292..2580
                     /locus_tag="AC16CY_02550"
                     /inference="COORDINATES: protein motif:HMM:NF019344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3352686.1"
                     /translation="MDDFDISPDRIGESAQRVLDRAVEESRRRGHAEVTNEDVFLAFA
                     RVEWDTYSQVMRELQLNPHEIVRALSEYLDSLPKDPEGTTRVAAPTKLVLKFALHRAS
                     RAGRQAVESDDLLSAVVEESQGVAVSIIRRHGVEPDVLASRIATKMRDNEVREEHLRK
                     RFELPPFLKNFATNLNLLARQDRIAPVYSREAEIQQVLEILCHRERSNSVMLIGEPGV
                     GKTAVVEGLARRIEFEPETLPIRLRECQVINLQMNSMVAGTMLRGMFEDRIQNVIREI
                     KERPNLILFVDEAHTMVGAGSALGAPADAANVFKSVLARGEVRIIGATTLSEYKEYIQ
                     EDEALARRFRTVVVAEPTVEQTRRILYSVRPRLERNYSVRLLDDAIETALELSSRYQR
                     HLHLPDKAIGWLDTAAVRAEMDNRWDVTAADVVEVISNAAQIPKDMVFRDVGERFKDI
                     ESRLAGRVIGQKKAVEALARRLVLNKGPLKDGFDRPDGVLLFLGPTGVGKTELAKALA
                     EFLFGDEKRMIRIDMSEYQDSGVSVDKLIGMPRGIVGSQRGGILTNQLRDNPCTVVLL
                     DEVEKASPTLLNLFLQAFDEGWLTDGRGKRVYLSDAIVIMTSNAGSEHFRKLQNPLGF
                     RSGQEGDGLNQVQADINRELERRFPPEFRNRIDQVVVFEPLTKDEVRQIAVKYLDEVS
                     ASLRRWNKMLLVEPEALDKLVADGHSLAYGARFLKRVIDDQIKMPLSQQWKDSNTFRA
                     VVRDDRIVVESAGPQLVASIAV"
     gene            complement(2567..3550)
                     /locus_tag="AC16CY_02555"
     CDS             complement(2567..3550)
                     /locus_tag="AC16CY_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPL family radical SAM protein"
                     /protein_id="HIR3352687.1"
                     /translation="MEIAPLLFDLGKSRPTTRQVANIVRTGGPDALTEATRRADAARY
                     QEVSCRSALNPVKGMPFNWTLNPYRGCTHGCHYCYARRYHAQFELGSDDEFASVILVK
                     TNIVDVLRRELRRPRWTRELVAVGSATDCYQPIEGLYKLTRGALEAFCEFDTPVGLVT
                     KGPMIVRDKDVLADMSRRAGCSVYLSVPCVDEDVWRSLEPGTAHPLQRLKAVRELVDA
                     GIRAGVLMNPIVPGISSRPALLERTMKAIADHGARFVGCNVMYLQDGTRDHFMRWLAD
                     EHPELVDGYERLYTAKYPPMGYRKEVTSLVSALKKKYGLARDHEVPGSTPR"
     gene            complement(3617..>4227)
                     /locus_tag="AC16CY_02560"
     CDS             complement(3617..>4227)
                     /locus_tag="AC16CY_02560"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3352688.1"
                     /translation="SAVYAPSDAPVREDASLAPASPYALSKLAQEQLALRACRDDRLD
                     IVIARPFNHTGPRQKPSFVAPAMARQVALIERGLVEPVISVGNLDAKRDLSDVRDVVR
                     AYSALMERGRSGDVYNVGSGVGHSIRSLLEALLSRSEIPVRIETDPARLRPTETPYPV
                     ADTSRLRAETGWQPQWSFERMLDDLLAYWRGQVRSAKFSVET"
BASE COUNT          764 a         1346 c         1420 g          697 t
ORIGIN      
        1 ggaaacggct gcggcgtctg cccgatcgcg ggccacgcaa gcgccacgct caacagggtc
       61 gcagacagaa cgtgtcgcac ggtacatccc attataaata ccggccggga tccgtcagcc
      121 ccagccaatc cagccgaatg ttccacggtg cgtccccgag tgggcaacct tgcacagttc
      181 gcggcacttc gtgcgggatg tggcccgatt ccgggccagt ccggttggat cgcatcttgc
      241 gaaagataca ggcggctgag gttcacgccg aaagccagga ggggtcccca aatggacgac
      301 ttcgacatct caccggaccg catcggtgaa tcagcgcaga gggtactcga ccgcgctgtc
      361 gaagagtctc ggcgccgcgg tcacgctgag gtcaccaacg aagacgtctt cctcgcgttt
      421 gcgcgggtgg agtgggacac ctactcgcag gtgatgcggg agctgcagct gaacccgcac
      481 gagatcgtcc gtgcgctctc tgagtacctc gattcgctgc cgaaggaccc ggaggggacg
      541 acgcgcgtcg cggctccgac aaagctcgtg ttgaagtttg cgctgcaccg cgccagccgc
      601 gccggccgtc aggctgtcga gtcagatgac ttgctgtcgg cagtcgtcga agagagccag
      661 ggcgtagccg tatcgatcat tcgccggcac ggcgtcgagc ccgacgtgct cgcgtcgcgg
      721 atcgcgacca agatgcgcga caacgaggtg cgcgaggagc acctccgcaa acgcttcgag
      781 ctgccgccgt tcctcaagaa cttcgcgacc aatctgaacc tgctcgcgcg gcaggaccgg
      841 atcgcgccgg tctacagccg cgaggccgaa atccagcagg tgctcgagat cctctgtcac
      901 cgcgagcgct ccaactccgt gatgctgatt ggcgagcccg gagtaggcaa gacggccgtc
      961 gtcgaggggc tcgcccggcg catcgagttc gagccggaaa cgctgcccat ccgtcttcgg
     1021 gagtgccagg tcatcaacct gcagatgaac tcgatggttg ccggcacgat gctgcgaggc
     1081 atgttcgagg accgcatcca gaacgtgatc cgcgaaatca aggagcggcc caacctcata
     1141 ctgttcgttg acgaagcgca cacgatggtg ggggccggct cggcgctggg cgccccggcc
     1201 gacgccgcca acgtcttcaa gtcggtgctc gcgcgcggcg aagtgcggat catcggcgcc
     1261 accacgctga gcgagtacaa ggagtacatc caggaagacg aggcgctcgc gcggcggttc
     1321 cgaacggtgg tggtggcgga accaaccgtc gagcagactc gccggattct ctacagcgtg
     1381 cggccgaggc tcgagcgcaa ctactcggtt cggctcctgg acgatgcgat cgagaccgcg
     1441 ctggagttgt cgtcgcgcta ccagcgccac ctgcacctgc ccgacaaggc gatcgggtgg
     1501 ctcgataccg cagcggtgcg cgccgagatg gacaaccgct gggacgtgac ggcggccgac
     1561 gtcgtcgagg tgatctcgaa cgcggcgcag atcccgaaag acatggtgtt tcgagacgtc
     1621 ggagagcggt tcaaggacat cgagtcgcgg ctggccggcc gcgtcatcgg ccagaagaag
     1681 gcggtcgagg cgctcgcgcg caggctcgtg ctcaacaagg gaccgctcaa ggacggcttc
     1741 gatcggcccg acggcgtgct gctgtttctc ggcccgaccg gagttggaaa gaccgagctg
     1801 gcgaaggcgc tcgccgagtt cctcttcggc gacgagaagc gaatgatccg gatcgacatg
     1861 tccgagtacc aggacagcgg cgtgtcggtg gacaagctga ttggtatgcc ccgcggcatc
     1921 gtcggcagcc agcgcggcgg catcctcacc aatcagctcc gggacaaccc gtgcaccgtg
     1981 gtgctgctcg acgaggtcga gaaggcgagc ccgacgctgc tgaacctctt cctgcaggca
     2041 ttcgacgaag gctggttgac agacggccgc ggcaagcgcg tctacctctc cgatgccatc
     2101 gtcatcatga cgtcgaacgc gggctccgag cacttccgca agctgcagaa cccgcttgga
     2161 ttccgttcag ggcaggaggg ggacgggctc aaccaggtgc aggccgacat caaccgcgag
     2221 ctcgagcggc ggttcccgcc cgagttccgg aaccgcatcg atcaggtcgt cgtgttcgag
     2281 ccgctgacca aggacgaagt tcggcagatc gcggtgaagt acctggacga ggtgagcgcg
     2341 tcgctgcggc ggtggaacaa gatgctgctc gtcgaacccg aggcgctgga caagctggtg
     2401 gccgacggcc acagcctggc ctacggcgcg cgcttcctca agcgcgtcat cgacgaccag
     2461 atcaagatgc cgctcagcca gcagtggaag gactcgaaca cgttccgggc cgtcgtgagg
     2521 gacgaccgga tcgtcgtgga atcggcggga ccgcagctgg tggcctctat cgcggtgtag
     2581 aacccggcac ctcgtggtcg cgtgccaggc cgtacttctt cttcagggcc gagacgagtg
     2641 acgtgacctc cttccggtag cccatgggcg ggtacttggc ggtgtacaag cgctcgtagc
     2701 cgtcaaccag ttccgggtgc tcgtcggcga gccagcgcat gaagtgatcg cgcgtgccgt
     2761 cctgcaagta catcacgttg cagccgacga accgcgcgcc gtggtccgcg atcgccttca
     2821 tcgtccgctc gagcagcgcg ggtctcgacg agatgccagg cacgatcgga ttcatcagca
     2881 ctccggcgcg gatgccggcg tcaacgagct cgcgaacggc cttcagccgc tgaaggggat
     2941 gggcggtgcc cggctcgagc gagcgccaga cgtcctcgtc cacacagggc acgctcaggt
     3001 acacgctgca cccggcgcgc cgcgacatgt cggcgaggac gtccttgtcg cggacgatca
     3061 tcggcccttt cgtcaccagg ccaaccggtg tgtcgaattc gcaaaaggct tcgagcgcgc
     3121 cgcgcgtcag cttgtacaga ccttcaatcg gctggtagca gtcagtggcg ctgccgacgg
     3181 cgacgagctc cctcgtccat cgcgggcgtc gaagctcccg cctcaggacg tccacgatgt
     3241 tggtcttcac caggatcacg gatgcaaact cgtcgtccga gccgagctcg aattgcgcgt
     3301 gataccggcg cgcgtagcag tagtgacacc cgtgagtgca gcccctgtac ggattgagcg
     3361 tccagttgaa cggcatcccc ttgaccgggt tgagcgcgga acggcagctg acttcctgat
     3421 acctggctgc gtccgcccgg cgggttgcct cggtcagggc gtcgggcccc ccggtcctga
     3481 cgatgttcgc gacctggcgt gtggtgggcc tggatttgcc gagatcgaac agaagcgggg
     3541 caatttccat ggcagtcctt ggtcttacgc cgtatgttcg ccccagagac taccagaagc
     3601 cggaatcact ttccacttac gtctcgacgc tgaactttgc acttcgaacc tgcccgcgcc
     3661 agtaggcgag cagatcgtcc agcatccgct cgaacgacca ctggggctgc catccggttt
     3721 cggcgcggag gcggctcgtg tcggccaccg ggtacggtgt ctcggtggga cggagacggg
     3781 cgggatcggt ttcgatgcga accgggattt ccgatcgcga cagaagggct tcgagaagcg
     3841 atcgaatcga gtgcccgact cccgatccga cgttgtagac atcgcccgag cgaccgcgtt
     3901 ccatcaacgc cgagtacgcg cgaacgacgt cgcgaacgtc ggagagatct cgcttggcgt
     3961 cgaggttgcc cacgctgatc accggttcca cgaggccgcg ctcgatgagg gcgacctgcc
     4021 gtgccatggc gggtgccacg aacgacggtt tctgccgggg gccggtgtga ttgaagggcc
     4081 gcgcgatgac gatgtcgagg cggtcgtctc tgcacgcgcg cagcgccagc tgctcctgcg
     4141 ccagcttgct gagcgcgtaa gggctggcgg gcgcgagcga cgcatcctcg cggacagggg
     4201 cgtctgaagg cgcgtacacc gccgacg
//
LOCUS       DBNGSH010000116         2917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4252986, whole genome shotgun sequence.
ACCESSION   DBNGSH010000116 DBNGSH010000000
VERSION     DBNGSH010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4252986"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="AC16CY_02565"
     CDS             complement(<1..455)
                     /locus_tag="AC16CY_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3352689.1"
                     /translation="MEREISKLVSRFEQGRITRRELIQAIAALAALSTTASAAAPLRA
                     GSINHVSVLVSDLQRSIDFYNRVFSLSVINEDKANKIVRLGIDKKILVSIRLEPPPGL
                     VDHFAIGVDNFNRDAVTRDLKEMGLTPQENIEFGFHVKDPDGANVQITG"
     gene            complement(539..1999)
                     /locus_tag="AC16CY_02570"
     CDS             complement(539..1999)
                     /locus_tag="AC16CY_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3352690.1"
                     /translation="MSGSIVYAFGSFELDAAARRLTRAGESVPMSDRHVGVLLELVSR
                     PGAIVSKDAIIDAVWRGVAVTDNSLEQAISAIRRVIGDGSGGTPGIQTVARQGYRFIG
                     TVTKTAARASGDALEALLAPHRAWIEGRAALETLKRDQILHAREVFERVLRDVPDEAS
                     AHVGLANALVLLFETTRVDPAPDVAALEGAVRHAREACQLQTGYGEAWATLGFVLDRI
                     GRPMDALAASRRAIALEPDNWRHHFRLSYVGWGEERLRSAHRVLTLLPGFPLAHWLAA
                     TVHVARQAFDDADRELASAVAGEAPAAEGHARFSAIALHWLRGLIHLARGDDVHALAE
                     FEKELANETSGHLYARECCANTWYAIGALRLRQGRSQEARAAFEQCIERVALHPGARL
                     GIAAAAPQRVAGVSQPVAESAVAQLRPVDAALWRAAGHVLAGSHGEAAALMESALADA
                     PAGNGGWLLPVEPLLRVHAAPDVWAGALARLRARAA"
     gene            2070..>2917
                     /locus_tag="AC16CY_02575"
     CDS             2070..>2917
                     /locus_tag="AC16CY_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3352691.1"
                     /translation="MALKKCFVVVGALMLAASAAWSRGPALVAQGARARGLPIFEVDA
                     AWPRVPEKWKLGDASSFAIDAQDNVWLLHRPRTLPPEQAATAAPPVIVFDPSGKFVKA
                     WGGAGSGYEWPEREHGIHIDGKGFVWLGGNNCPTNGIAGLKPVADDQLLKFSPEGKLM
                     LQIGRSNQSKGNADTRNVHRAADAWVHAPTNEVFVADGYGNHRVIVFDADTGAFKRMW
                     GAFGDRPMDDDHCDVVTPKTVDDPGPRSFSTVHALRVARDGTVYVADRENRRIQAFTQ
                     DGKFVK"
BASE COUNT          466 a          998 c         1004 g          449 t
ORIGIN      
        1 atcccggtga tttgcacgtt tgcgccgtcg ggatccttta catggaaccc gaactcgatg
       61 ttctcctgcg gtgtcagccc catctccttc aaatcgcgcg tgaccgcgtc cctgttgaag
      121 ttatcgacgc cgatggcgaa gtgatcgacg agcccgggcg gcggctccag gcggatcgat
      181 acgagaatct tcttgtcgat cccgagcctc acgatcttgt tggccttgtc ctcgttgatc
      241 accgacaggc tgaacacgcg gttgtagaag tcgatcgagc gctgcaggtc gctgacgagc
      301 accgagacgt gattgatgct gccggcgcga agcggggcgg cggccgaggc cgtcgtgctc
      361 agggccgcga gggccgcgat cgcctgaatc agctctcgcc gcgtgatccg gccctgctcg
      421 aagcggctca ccagtttcga aatctcgcgt tccatacgat ctcctctgcg ttgcgtagag
      481 gcgggtcttc cctggcctga gcgaagtcga aggcagaccc gcctggtgac agtgcccgtt
      541 acgcggcgcg tgcacgcagc cgcgccaggg cgccggccca gacgtcgggc gcggcgtgta
      601 cgcgcagcag cggctcgacc ggcagaagcc atcccccgtt tccagcgggc gcgtcggcca
      661 gcgcgctctc catcagcgcg gcggcttcgc cgtgcgagcc tgcgagcacg tgaccggccg
      721 cccgccatag cgccgcatcg accggccgaa gttgagcgac tgccgactcg gcaacgggct
      781 gtgacacgcc ggccacgcgc tgcggcgcag ccgccgcgat gccgagccgc gcgcccgggt
      841 gcagcgccac gcgctcgatg cactgttcga aggcggcgcg cgcctcctgc gatcgcccct
      901 gccgcagccg gagcgcgccg atggcgtacc acgtgttcgc gcagcactcg cgcgcgtaca
      961 ggtgtccgct ggtttcgttt gccagctcct tttcgaactc ggcgagcgcg tgcacgtcgt
     1021 cgccccgcgc gagatggatc aacccgcgca gccagtgcag ggcgatggcg ctgaaccggg
     1081 cgtgaccctc cgccgccggc gcctcgccgg cgacggccga cgccagctca cgatcggcgt
     1141 cgtcgaacgc ctgacgcgcc acgtgcacgg tggcggcaag ccagtgcgcc agcggaaacc
     1201 ccggcagcag cgtgagcacg cggtgcgcgg atcgaagtcg ctcttcgccc cagccgacat
     1261 aggacaggcg gaagtggtgc cgccagttgt cgggctcgag cgcgatcgcg cgcctcgagg
     1321 ccgcgagcgc gtccatggga cggccgatcc gatcgagcac gaacccgagc gtggcccagg
     1381 cctcgccata gccggtctga agctgacagg cttcgcgtgc gtggcggacg gctccttcga
     1441 gcgccgcgac gtcaggcgcg ggatcgacgc gcgtcgtctc gaacagcagg acgagcgcgt
     1501 tggcgagtcc gacgtgcgcc gacgcctcgt cgggaacgtc gcgtagcacg cgctcgaaga
     1561 cctctcgcgc gtgaaggatc tggtcgcgct tcagcgtctc gagcgccgcg cgcccttcga
     1621 tccaggcgcg atgcggagcg agcagcgcct cgagcgcgtc gccgctggcg cgggcggccg
     1681 tcttcgtgac ggtgccgatg aaccgatatc cctgtcgcgc gaccgtctgg atgcctggcg
     1741 tcccgcccga gccgtcgccg atcacgcggc ggattgccga tatcgcctgt tcgaggctgt
     1801 tgtcggtcac cgcgacgccg cgccacacgg cgtcgatgat cgcgtccttg gaaacgatcg
     1861 ctccgggtcg cgacaccagc tcgagcagca cgcctacgtg ccggtccgac atcggcacgg
     1921 actctccggc acgcgtcagc cgccgtgcgg ccgcgtcgag ctcgaaggag ccgaaggcgt
     1981 agacgatact tcctgacaac gcatcagatt gtagcggcca gcccgcgaaa tcgggttcgg
     2041 gagtatgctc ctggccgcaa ggattcgata tggctctcaa gaagtgcttc gtcgtcgtcg
     2101 gtgcattgat gctcgccgca agcgccgcct ggagccgcgg ccccgcgctg gtcgcccagg
     2161 gcgcgcgggc acgcggcctc ccgatcttcg aggtggacgc cgcctggccc agggtgcccg
     2221 agaagtggaa gctcggcgac gcgtccagct tcgcgatcga cgcgcaggac aacgtgtggc
     2281 tcctccatcg tccgcggacg ctgccgccgg agcaggccgc cacggccgcg ccgcccgtca
     2341 tcgtgttcga tccttcgggc aagttcgtca aggcgtgggg cggcgccgga agcggttatg
     2401 aatggccgga gcgcgagcac ggcattcata tcgacggaaa aggatttgtg tggctcggag
     2461 gaaacaactg cccgaccaac ggcatagccg gcctcaagcc ggtcgccgac gatcaactgt
     2521 tgaagttctc gccagagggc aagctgatgc tgcagatcgg ccgcagcaac cagagcaagg
     2581 ggaacgccga cacccggaac gtgcatcgcg cggcggatgc atgggtccac gcgccgacca
     2641 acgaggtgtt cgtcgccgat ggctacggca atcaccgggt gatcgtgttc gacgcggaca
     2701 cgggggcgtt caagcggatg tggggcgcgt tcggcgatcg tccgatggac gacgaccact
     2761 gcgacgtcgt gacgccgaag accgtcgacg atcccggtcc gcgcagcttc agcaccgtcc
     2821 acgccctccg cgtggcgaga gacgggaccg tgtacgtggc cgaccgggag aaccggcgca
     2881 tccaggcgtt cacgcaggac ggaaagttcg tcaagca
//
LOCUS       DBNGSH010000117         3284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1875938, whole genome shotgun sequence.
ACCESSION   DBNGSH010000117 DBNGSH010000000
VERSION     DBNGSH010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1875938"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="AC16CY_02580"
     CDS             complement(<1..1244)
                     /locus_tag="AC16CY_02580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3352692.1"
                     /translation="MKWTLALAACLVIGQGTAAAERAASPADATVFIRLVGSVHAELE
                     EPGSAKQTLDLDKIEIGTGSGFVITPYGHVLTNEHVIRNTQFDVASGPRKARITLKVS
                     SILVCLPAATAAARGDASPCYNASVHSSDAALDLAVLFVAADLPYLALGDSDAVRSGQ
                     PVDALGYPFGRQLEVGRVAAPDLVPEVSTSPSTISALRSGDAGDRRAVQINGTVNPGN
                     SGGPLVDRDGFAVGVIRARVKGDAGIAFAIPINQAKDFLESRGLESLMPVTRIRLGAM
                     QHFEGKGVALLLPEGVDDTSPLRSRVESDADGGAVALRIDRAVSPWTARQLERELTTT
                     ESFEEIAVEASESQMSKAGGAPLLLGRASGTLAGRDQPVRMQYAILELGTEKLVARYV
                     GSAEQLAYNESVLRNSLQSLEG"
     gene            1421..2443
                     /locus_tag="AC16CY_02585"
     CDS             1421..2443
                     /locus_tag="AC16CY_02585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3352693.1"
                     /translation="MKKIGFLSFGHWTPSPHSEVRTASDALLQSIDLAVAAEELGADG
                     AYFRVHHFARQLGSPFPLLAAVGARTKKIEIGTAVIDMRYENPLYMAEDAGAADVIAG
                     GRLQLGISRGSPEQVIDGWRYFGYAPAEGETHQDMARRHAEVFLEVIQGRGFAQPNPR
                     PMFPNPPGLLRVEPHSEGLRDRIWWGSSSNATSRWAAKLGMHLQSSTLKDDESGEPLH
                     VQQRKQIEAYRDAWKEAGHTREPRVSVSRSIFALVDDRDRAYFGGDARSQDQVGYIDA
                     NTRAIFGRSYAAEPDALVKQLAADEAIAAADTLLLTVPNQLGVDYNVHAIGSILKLVA
                     PELGWR"
     gene            2536..>3284
                     /locus_tag="AC16CY_02590"
     CDS             2536..>3284
                     /locus_tag="AC16CY_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352694.1"
                     /translation="MARTRAGTAFSAVVIACIVIGAGGCRRSPPSAVGEPGAYAVDAG
                     GRPITQLEAGSSLHIGGRSLKPSTMYEFRLAIGDQPAQALDRAVSFARSSSDRDGNVP
                     PFVLWYQAGVVGCSDRPKLDGRPYTFRSFDEAEKALAGQTLTVSVHEVNRDPTGRAQP
                     MALKAGVPIVVSRLPIARRTSPAVFVSDAKGCLLNSQLSNDGDMYVSGRNFRPGEAVE
                     LSVVANQRSWFVNDRIQDLTGVGAVAAPERV"
BASE COUNT          523 a         1148 c         1105 g          508 t
ORIGIN      
        1 tgaccctcga ggctctgcaa cgagttgcgc agcacgcttt cgttgtacgc gagctgctcc
       61 gccgagccga cgtaacgcgc gacgagcttc tccgtcccga gttcgagaat ggcgtattgc
      121 atccggaccg gctggtctct ccccgcgagc gtcccgctcg cgcgtcccag cagcagcggc
      181 gctccaccgg ccttcgacat ctgactctcg ctcgcctcga ccgcaatttc ttcgaacgat
      241 tcggtcgtcg tcagctcgcg ctcgagctgc cttgccgtcc acggcgacac cgcccgatcg
      301 atccgcagcg caacagcgcc gccgtccgcg tcgctctcga cgcgggaccg cagcggcgac
      361 gtgtcgtcca cgccttccgg cagcagcagc gcgacgccct ttccctcgaa gtgctgcatc
      421 gcgccgagac gaatgcgcgt cacgggcatc aacgactcga gcccccggga ctcgagaaag
      481 tccttggcct gattgatcgg aatcgcgaac gcgatcccgg catccccctt gacccgcgcg
      541 cggatgacgc ccacggcaaa gccgtctcga tcgacgagcg ggccgccgct gttccccggg
      601 ttgacggtgc cgttgatctg cacggcgcgg cggtcccccg cgtcgccact gcgcagcgcc
      661 gagatcgtgc tcggcgacgt gctgacttca ggcacgaggt ccggtgcggc gacccggccg
      721 acctcgagct ggcggccgaa gggatagccc aacgcgtcca cgggctgtcc cgaacggacg
      781 gcgtccgaat caccgagcgc aaggtaagga aggtcggctg caacgaagag cacggcgaga
      841 tcgagcgcgg catccgacga gtgcacgctt gcgttgtagc aaggcgacgc gtccccgcgg
      901 gccgcagcgg ttgcggctgg caggcacacc aggatcgaag acaccttcaa ggtgatcctg
      961 gccttgcgtg ggccgctcgc cacgtcgaac tgagtgttgc ggatcacgtg ctcgttggtc
     1021 agcacgtgcc cgtagggcgt gatgacgaag ccgctgccgg tgccgatttc gatcttgtcc
     1081 aggtcgagcg tctgtttcgc cgatccgggc tcctccagct cggcgtgcac gctgcccacg
     1141 agccggatga ataccgtggc gtcggccggg gacgcggcgc gctccgcagc ggccgtaccc
     1201 tggccgatga ccagacaagc ggcaagtgcc agcgtccact tcatagaatg cgctgcagag
     1261 atcaacgaac ccagctggga gaacgaactt tgcggtttcc tcgctgattc tacccggccg
     1321 gcgcggcgtt gttccgcggc ctcgccgggt tggccacgat atgcttgtcg ccctgctcat
     1381 ggtcgggcag acaggcggtc gagtcgggga ggaaatgcgg gtgaagaaaa tcgggttcct
     1441 gtcgttcggg cactggacgc cgtcgcccca ctcggaggtg cgcacggcgt cggacgcgct
     1501 gctccagtcg atcgacctgg ccgtggccgc cgaggaactg ggcgccgacg gcgcctactt
     1561 ccgcgtccac cacttcgccc gccagctcgg ctcccccttc ccgctcctcg cggccgtggg
     1621 cgcgcgcaca aagaaaatcg agatcgggac ggccgtcatc gacatgcgct acgagaaccc
     1681 gctgtacatg gccgaggacg ccggggccgc cgacgtcatc gctggcggcc ggctgcagct
     1741 gggcatcagc cgcgggtcgc cggagcaggt gatcgacggc tggcgctact tcggctacgc
     1801 gcccgccgag ggcgagacgc accaggacat ggcccgccgc cacgccgagg tcttcctcga
     1861 ggtgatccag ggcaggggct tcgcgcagcc gaacccgcgc ccgatgttcc ccaacccgcc
     1921 ggggctgctg cgcgtcgagc cgcattccga ggggctgcgc gatcgcatct ggtggggctc
     1981 cagctcgaac gccacctcca ggtgggccgc gaaactgggc atgcacctcc agagctccac
     2041 gctcaaggac gacgagagcg gcgagccgct gcacgtgcag cagcgcaagc agatcgaggc
     2101 gtaccgcgac gcgtggaagg aggcggggca cacgcgcgag ccgcgcgtct cggtgagccg
     2161 cagcatcttc gcgctcgtgg acgaccgcga ccgcgcctac ttcggaggcg acgcccgcag
     2221 ccaggaccag gtcggctaca tcgacgcgaa tacccgcgcc atcttcggcc gctcgtacgc
     2281 cgccgaaccg gacgcgctcg tcaaacagct cgccgcggac gaggccatcg ccgccgccga
     2341 cacgctgctc ctgaccgtcc cgaaccagct tggcgtggac tacaacgtcc acgccatcgg
     2401 cagcatcctg aaactcgtcg ccccggagct tggctggcgc tgaccgcgat cgggatcagg
     2461 gcttgttcga atcgtgcgag tccctcgttt tcataggtac atcaccacag cctgaaccgc
     2521 atcaggagtc gacgaatggc tcgtactcgc gccggaaccg cgttttctgc agttgtcatc
     2581 gcgtgcatcg tcatcggcgc agggggctgc aggaggtcgc ctccttctgc ggtcggtgaa
     2641 cccggtgcgt acgcggtcga cgccggcggg cgcccgatca cgcagctgga ggcgggatcg
     2701 tcgctgcaca tcggcggcag aagcctgaaa ccgtcgacga tgtacgaatt ccggctcgcc
     2761 atcggcgacc agcctgcgca ggcgctcgac cgggccgtca gctttgcccg ttcgtcgagc
     2821 gatcgggacg ggaacgtgcc gcccttcgtc ttgtggtacc aggccggcgt ggtcgggtgc
     2881 tcggatcggc ccaaactgga cggccggccg tacacgttcc ggagcttcga tgaggcggag
     2941 aaggcgctgg ccggtcagac gctgaccgtc tcggtccacg aggtgaaccg cgatcccacc
     3001 ggacgcgcgc agccgatggc gctcaaagcg ggcgtgccga tcgtcgtgtc gcgcctgccg
     3061 atcgcgcgcc ggacgagccc ggccgtgttc gtgtccgacg ccaagggctg cctgctgaac
     3121 agccagctgt cgaacgacgg cgacatgtac gtgagcggca ggaacttcag gccgggcgag
     3181 gccgtcgagc tgtcggtcgt cgcgaaccag cgctcctggt tcgtcaacga ccggatccag
     3241 gacctgaccg gcgtgggggc cgtggccgcg ccggagcgcg tcca
//
LOCUS       DBNGSH010000118         3569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4593076, whole genome shotgun sequence.
ACCESSION   DBNGSH010000118 DBNGSH010000000
VERSION     DBNGSH010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4593076"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="AC16CY_02595"
     CDS             complement(<1..1461)
                     /locus_tag="AC16CY_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352695.1"
                     /translation="MTRPVLMRVSDRWFRLLLRLYPADFRDEMGDGFLETYTERAHVT
                     VARRGARGLTWLWLRALLDALRNGPAERLRPAASWRRPGNWGRDIELARRRLLRSPGF
                     VAATLTTLTVGFGAFAVVYTALDKIVLAPMPYHEPDDLYFVWRDQSALGGVSRDWLAG
                     PDVADLQNAGGVIEGAAGMRLSVPALSLGPDGEPHQVLAMLTSPNLFELLGVTPALGR
                     GFAADEVGPTRPPIVVLSDVLWRRLGGDPSIVGSQVWLSGVPYTVIGVMDPAFRFVRH
                     AALGPPQEPDLYLPFAFHLADQDPRDPNHTTFAALMRVRAGTLSDRVAAAVDAVERVV
                     NARNRQTPGVRLHAIRLQDDLIAPIRPVLVTLGLAGVFLLLVLTVNLASLLLARAGAR
                     EREFAVSRALGANGAAVLRAMVIEGGALGLMGGVAGALTGSWGGRVLVSLAPLDLPRR
                     SEIALDWRVAGVIVAAGIGVGVLAAALPAVWISRASL"
     gene            complement(1458..1811)
                     /locus_tag="AC16CY_02600"
     CDS             complement(1458..1811)
                     /locus_tag="AC16CY_02600"
                     /inference="COORDINATES: protein motif:HMM:NF015513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3352696.1"
                     /translation="MTSDRDRIDAHLPLRPVEFHILLSLAAGHRHGFGIIEDIEARQD
                     VGVPDVGTMYRALARMVESGLIEAASSPESADPRRSYYAITARGTRVAKAEARRLEAL
                     TRAARVGGLLDKKTT"
     gene            2470..2715
                     /locus_tag="AC16CY_02605"
     CDS             2470..2715
                     /locus_tag="AC16CY_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352697.1"
                     /translation="MSIQITPEQEARINELARQTGRDTTEVLGEVIDSYFEELARINA
                     TLDRRYDEIKSGQVKPISGDEVLERLQRRSGERRARG"
     gene            2712..2966
                     /locus_tag="AC16CY_02610"
     CDS             2712..2966
                     /locus_tag="AC16CY_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352698.1"
                     /translation="MNEFAFHPEAEVDLIEIWDFIAEDSVDAADRVRDDILGAVVRAS
                     IALCWREGRAARRPFRQPHAQKAPLIVLIVRSFGSRVACS"
     gene            complement(2961..3284)
                     /locus_tag="AC16CY_02615"
     CDS             complement(2961..3284)
                     /locus_tag="AC16CY_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3352699.1"
                     /translation="MPGVASHAAAARKVTIRLMLKAALAKTKSASTVVSPRSFTLRSP
                     AIVLSQGHRRDDLTSRPLRFWRVYDYLIAYAPEKPLLVLAVLHGRRNPRILAGILRGR
                     EQAHS"
BASE COUNT          682 a         1193 c         1159 g          535 t
ORIGIN      
        1 cagcgaggcg cgcgatatcc acacggccgg cagtgccgcg gccagaacgc caacgccgat
       61 gcccgcggcg acgatcacgc cggcgacgcg ccagtcgagc gcgatctcgc tgcgacgagg
      121 cagatccagc ggcgcgagtg agacgagcac acggcccccc cacgaacccg tgagcgcgcc
      181 ggccacgcca cccatcaggc ccaacgcccc gccttcaatc accatcgccc gcagcacggc
      241 cgcgccattg gcacccagcg cccgcgacac cgcgaactcc cgctcgcgtg cgcccgctcg
      301 cgccagcagc agcgaggcga ggttcacggt gagcacgagg agcaggaaca cgcccgccag
      361 accgagcgtg accagcacgg gtcggattgg cgcgatcagg tcgtcctgca ggcggatcgc
      421 atggagccgc acccctgggg tctgtcggtt gcgcgcattg acgacgcgct ccacggcatc
      481 gaccgccgcc gccactcggt ccgatagggt gccggcgcgc acgcgcatca gggcggcaaa
      541 cgtggtgtgg ttcggatcgc gcggatcctg atcggcgaga tgaaacgcaa acggcaggta
      601 cagatccggt tcctgcggcg gaccgagcgc ggcgtgccgc acgaatcgga aggccggatc
      661 catgacgccg atcacggtgt aaggcacgcc gctcagccac acctgcgacc cgacgatcga
      721 cgggtcgcct ccgagccgtc tccagagtac gtcgctgagg acgacgatag ggggacgcgt
      781 gggcccgact tcgtcggcgg caaatccacg accgagcgcc ggtgtgacgc ccagcagctc
      841 gaacaggttg ggcgaggtga gcattgccag cacctggtgc ggctcaccat ccggcccgag
      901 cgacagcgcg ggcaccgaga gccgcatccc ggcggcgccc tcgatgacgc cgccggcgtt
      961 ctgcaggtcg gcgacgtctg gtcccgcgag ccaatcgcgt gacacgccgc cgagcgccga
     1021 ttgatcgcgc cagacgaaat acaagtcgtc cggctcgtga tacggcatcg gggccaggac
     1081 gattttgtcc agcgccgtgt agaccacggc gaatgcgcca aagccgacgg tcaatgtcgt
     1141 caaggtggct gcgacgaagc cgggtgagcg caggaggcgc cggcgcgcca gctcgatgtc
     1201 gcggccccag ttgccgggac gacgccacga ggcggccggc cgcagacgct cggcaggacc
     1261 attgcggagc gcgtccagca acgcacgaag ccacagccac gtcagtcccc tcgcgccgcg
     1321 gcgcgccacg gtgacgtggg cgcgctccgt gtaggtctcg aggaacccat cgcccatctc
     1381 atcgcgaaaa tccgcgggat acaggcgcag caacaggcgg aaccagcggt ccgacacacg
     1441 catgaggact ggccgcgtca tgtcgtcttc ttgtcgagga gaccaccgac tcgcgccgcg
     1501 cgcgtgagcg cctccagccg gcgcgcctcg gccttcgcca cgcgagtccc acgtgcggtg
     1561 atggcgtagt agctgcgccg cgggtcagcg gactccggcg aagatgccgc ctcgatcaag
     1621 ccgctctcga ccatccgcgc cagcgcgcgg tacatcgtgc ccacgtcggg cacccccacg
     1681 tcctgacggg cttcgatgtc ttcgatgatg ccgaagccat ggcgatggcc tgccgccagg
     1741 ctcaacagga tgtggaactc cacaggccgc aacggcaggt gggcgtcgat gcgatcgcga
     1801 tcggacgtca tcagaagtat gcgctgcgca tatatacacc agacatgcca gccgcgtgaa
     1861 gccgtttccg gcgaattgcc ggaggaacct cttacggtgg cgttcgctcg cgggacatcc
     1921 gtgtccggaa tcgcacgcac tcaggcctta cgtctatccg aaatcgactg tttgggttca
     1981 cagccgtatg acgggaaaag gtggtgtgaa aaagacggcg cgcgggagag ctacgaagac
     2041 actctgacga agctcgcgca ggatcgggga tcgagacggc gaccggagcg tcgaccgttt
     2101 tgcggcacaa catctcgatg ccagcaagag attcgctcac cgttcattcg cttcctggag
     2161 attccacgca actgtcgtac ggcacgtcga cgatgattgg gggtggctcc tcgggctgga
     2221 ctcgaaccag caaccctccg gttaacagaa gacgggagcg gaagcgacgg tgagcgacga
     2281 tctgcgctca agtgcttggg tcgtcttggt ttctgccgag tcagtcgatc accccagcag
     2341 tcagtcgatc tggatcgaaa caggtcgttt tcgaggggtt atgtcacagt tcccgtcaca
     2401 gctctcgcgc aggccaggtt cgcttgcatg acgggcccga gcctcgtcca gtgacctgag
     2461 caactcgcca tgtcgattca gatcacgccg gagcaggaag ccagaatcaa cgagctcgcg
     2521 cgtcaaaccg gacgcgacac gactgaagtc ctgggcgagg tcatcgacag ctacttcgaa
     2581 gaactggcca ggatcaacgc gacgctggat cgtcgctacg acgagatcaa gagtgggcag
     2641 gtgaagccga tcagcggcga cgaagtactc gagcgtctgc agcgcaggag cggggaacgt
     2701 cgcgcgcgcg gatgaacgaa ttcgcgtttc atccagaagc ggaagtcgac ctcatcgaga
     2761 tctgggactt catcgccgag gacagcgtcg acgctgccga ccgcgtccgc gacgacatct
     2821 taggggcggt cgtgcgggcg tcaatcgcgc tgtgctggcg ggagggcagg gccgcacgac
     2881 ggccattccg tcaacctcac gcgcagaaag caccgctaat cgttcttatc gtccgttctt
     2941 tcgggtcccg cgtcgcctgt tcatgagtgc gcctgctccc gtcctcgcag aataccagca
     3001 agaattcgcg gatttcgtcg accgtgaaga acggccagca cgaggagtgg cttttccggc
     3061 gcgtacgcga tcaggtaatc gtacacgcgc cagaatcgta acggacgcga agtcaggtca
     3121 tcccgtcgat gaccttggct caagacaatt gccgggctgc gcaaggtgaa gctgcgcggg
     3181 ctcacgaccg tggatgcgct cttcgtcttc gccagcgccg ccttcaacat caaacggatc
     3241 gtgaccttgc gcgcggcggc cgcatgagac gcgacgcctg gcacgctggc cacgattcgc
     3301 accgtccagc ggaacgcacg gaacgctggc cgtctttgtt tcgaaggcct ggcccacata
     3361 cgtgacagcg aatcggcctc gacgcacagg cacacacgtc cgcgccgcgg gctcccgcgc
     3421 cggaactcgc gcgacacgca ctagcggccc gttcgaggga tgggacgtcg tgctcgtcga
     3481 actgaacatc ccacctggcc gcacctcacc gggcccgggc catcgtcatc ccggtcccgt
     3541 cctcggctac atcgtcgaag gtcagatgc
//
LOCUS       DBNGSH010000119         2688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2046230, whole genome shotgun sequence.
ACCESSION   DBNGSH010000119 DBNGSH010000000
VERSION     DBNGSH010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2046230"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /gene="asnB"
                     /locus_tag="AC16CY_02620"
     CDS             <1..1193
                     /gene="asnB"
                     /locus_tag="AC16CY_02620"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HIR3352700.1"
                     /translation="ASELKAIATICETFEEFPPGHFLTRRDAKPVRYYTRDWFDVDGV
                     PDRTLEPSELKAALSDAVHAHLMTDVPYGVLLSGGLDSSVIGAVAAGYASKRVESGDH
                     DAAWWPRLHSFAIGLSGSPDVAAARRAADGIGTVHHSFEFTIQEGLDALRDVIWHIES
                     FDTTTVRASTPMYLMARRIRATGVKMVLSGEGSDEIFGGYLYFHKAPNARAFHEELVR
                     KLQLLHLYDCLRANKSMAAWGIETRVPFLDTRFLDIAMRIPARQKMVAPGSIEKKILR
                     DAFAGALPHEILWRQKEQFSDGVGYSWIDSLKAEAERRVSDRQMAAAPERFPVGTPLT
                     KESYVYRAIFDELFPHPAAAACVPSGPSVACSSPAALEWDPSFKANADPSGRAVKGVH
                     KEAS"
     gene            1190..1315
                     /locus_tag="AC16CY_02625"
     CDS             1190..1315
                     /locus_tag="AC16CY_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352701.1"
                     /translation="MRHQMPAGDKPGKPPSKPAPSPVPPPMPERDLPGGGKKKGR"
     gene            1319..>2688
                     /locus_tag="AC16CY_02630"
     CDS             1319..>2688
                     /locus_tag="AC16CY_02630"
                     /inference="COORDINATES: protein motif:HMM:NF021508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase effector Tle1 domain-containing
                     protein"
                     /protein_id="HIR3352702.1"
                     /translation="MALYAFDGTWNVRDTKDSVLRVQESQYGSDTGFRRETLETNVHR
                     FKEFYGPENAEYLEGVGTRFAVLGRIVGGGFGVGGKFRIRRMYRRLCERYQAGDTNID
                     IIGFSRGAAMALHFANLVSTHGIRNPKGPRSWLHYDTMFGWSIGMPKPSASDPRPPIR
                     FLGLFDTVASFGLPIGRLRNRRTGRWQVQKIPGNVGRSFHAMALDEVRAVFELVRPVR
                     ESEPERHYELWFRGVHSNIGGGYPDRGLSDIALGWMMEMYLWTLDKERPAAESGPAAI
                     PVRFGEALRLTQPERPSDPDWSGTALETLEPDADGELGRPSSLKRQRWRSLGARAAIH
                     YSAFRRTKNLLHDHYSQNRQLLRRIPADATPVYDPPFFYGSTPRQEAMLVAEEAFFHI
                     PVRAADWLSMDGLPVVRSDGWLGSGRTREEVANAQSRSAFVQVTAAWLLSGRRPVAEL
                     VLPKVV"
BASE COUNT          432 a          919 c          921 g          416 t
ORIGIN      
        1 tggcgtccga gctcaaggcg atcgcgacga tctgcgagac gttcgaggag tttccccccg
       61 gccacttcct gacgcgccgt gacgcgaagc ccgtccgtta ctacacgcgc gactggttcg
      121 acgtggacgg cgtgcccgat cgcactctcg agccgtcgga gctcaaggcg gcgctctcgg
      181 acgcggtaca cgcgcacctg atgaccgacg ttccctacgg cgtcctgctc tccggcggac
      241 tggattcctc cgtgattggc gccgtggccg ccggctacgc gtcgaagcgg gtcgaatccg
      301 gcgatcacga cgcggcgtgg tggccccggc ttcattcctt cgcgatcgga ctgtccggct
      361 cgccggacgt ggcggcggcc aggcgcgcgg ccgacgggat cggcacggtc catcactcgt
      421 tcgagttcac gatccaggaa gggctcgacg cgctccgcga cgtgatctgg cacatcgagt
      481 cgttcgacac gaccaccgtg cgcgcgtcaa cgccgatgta tctgatggcc cgccgcatcc
      541 gtgccaccgg cgtgaagatg gtgctctcgg gcgagggctc cgacgagatc ttcggaggct
      601 acctgtactt ccacaaggcc cccaatgccc gagcgttcca cgaagagctc gtgcgcaagc
      661 tccagctcct gcacctgtac gactgcctgc gcgcgaacaa gtcgatggcg gcgtggggca
      721 tcgagacgcg ggttcccttc ctcgacacgc gctttctcga catcgccatg cgcatccccg
      781 cgcgccagaa gatggtggcc cccgggagca tcgagaagaa gatcctgcgc gacgcgtttg
      841 ccggcgcgct cccccacgag atcctctggc gccagaaaga gcagttctcg gacggcgtcg
      901 gctattcgtg gatcgactcg ctgaaggcgg aggccgaaag gcgggtgtcc gatcgccaga
      961 tggccgccgc gccggagcgc tttccggttg ggacgccgct gaccaaggag agctacgtct
     1021 accgcgcgat cttcgacgag ttgttcccgc accccgccgc cgccgcgtgc gtgccgtccg
     1081 gcccgagcgt ggcgtgctcg tcgccggccg cgctcgaatg ggacccgtct ttcaaggcga
     1141 acgcggatcc aagcggtcgg gctgtgaagg gggtgcacaa ggaggcctcg tgagacatca
     1201 gatgccagcc ggcgacaagc cgggcaagcc cccttcgaaa ccggccccgt caccggtacc
     1261 gccgccgatg ccggagcgcg acctgccggg aggtggcaag aagaagggcc ggtagccagt
     1321 ggccctctac gcgttcgacg ggacgtggaa cgtccgcgac accaaggact cggtcctgcg
     1381 cgtgcaggaa tcgcagtacg gctccgatac cgggttccgg cgcgagacgc tcgagaccaa
     1441 cgttcatcgc ttcaaggagt tctacggccc ggaaaacgcc gagtacctcg agggcgtggg
     1501 cacgcgcttc gccgtgttgg ggcggatcgt cggcggtgga ttcggcgtcg gcggcaagtt
     1561 ccgcatccgc cgcatgtacc gccgcctctg cgagcgctac caggcgggcg acaccaacat
     1621 cgacatcatc ggcttcagcc gcggcgctgc catggcgctc cacttcgcga accttgtcag
     1681 cacgcacggc atcaggaacc cgaagggccc gcggtcctgg ctgcactacg acacgatgtt
     1741 cggctggtcg atcgggatgc cgaagccgag cgcgtccgac cccaggccgc caatcaggtt
     1801 cctcgggctg ttcgataccg tcgccagttt cgggctgccg atcggccggc tgcggaaccg
     1861 ccgcacgggg cgctggcagg tgcagaagat cccgggcaac gtgggccgca gctttcacgc
     1921 gatggcgttg gacgaagtgc gcgcggtctt cgagctcgtc aggcccgtcc gggagagcga
     1981 gccggaacgg cactacgagc tctggttccg cggcgtccac tcgaacatcg gcggcggata
     2041 cccggaccgc gggctctctg acatcgcgct cggctggatg atggagatgt acctgtggac
     2101 gctggacaag gagcggccgg cggcggagag cgggccggct gccatcccgg tgcgcttcgg
     2161 tgaagcgctc cgcttgacgc agccggagcg gccgtcggac ccggattgga gcggcacggc
     2221 gctcgagacg ctcgagccgg acgccgacgg cgagctcggc cgcccctcca gcctcaagcg
     2281 ccagcggtgg cgctccctcg gagccagggc cgcaatccat tactcggcgt tccggcgaac
     2341 gaaaaacctc ctgcacgatc attacagcca gaatcgacag ctgctgcggc ggataccggc
     2401 cgacgcgacc cccgtgtacg atccgccctt cttctatgga agcacgccgc ggcaggaagc
     2461 gatgctggtc gcggaggaag ccttctttca catcccggtc cgcgcggccg actggctgag
     2521 catggacggg ctcccggtgg tgcgcagcga cggatggctg ggcagcggca ggacccgcga
     2581 ggaggtggcc aacgcccagt cgcgatcggc cttcgtccag gtgaccgccg cctggctgct
     2641 cagcggccgg cggcccgtcg ccgagctggt cttgccgaag gtcgtgaa
//
LOCUS       DBNGSH010000120         5723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2555229, whole genome shotgun sequence.
ACCESSION   DBNGSH010000120 DBNGSH010000000
VERSION     DBNGSH010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2555229"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="AC16CY_02635"
     CDS             <1..861
                     /locus_tag="AC16CY_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3352703.1"
                     /translation="RFAGALFDGMRGMGRLWQAAGTVNAVLAPGLLEKAVEAGLRSLF
                     VGFETLNPDNLVEQRKYQNMRRDYAAAITRLHDLGVMINGSFVFGMDGDDGSVFERTV
                     EWAIEHGIETATFHILTPYPGTALYRRIADQGRLTTGDWDLYDTRHAVFRPARMTADQ
                     LECGYHWAYREFYRWRSIARGAMAHDDLLAGLRHAAYAAGWKKFEPLWDFVIRAKRAG
                     MMLPVLETILSEFGRRLPGSATRGDRRRTTSEEREQADHASVPQLLQIQPAISGSSAR
                     LQLTPAPPTS"
     gene            complement(836..3487)
                     /locus_tag="AC16CY_02640"
     CDS             complement(836..3487)
                     /locus_tag="AC16CY_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3352704.1"
                     /translation="MALAQGTRLGPYEITFQIGVGGMGEVYRAIDTNLGREVAIKVLP
                     ESFAQDGDRLARFEREAKTLASLNHPNIAIVHGFEKSSGMNGLVMELVEGPTLAERIT
                     EGPVPIEEALAIAKQIAEALESAHERGIVHRDLKPGNIKLRPDGTVKVLDFGLAKAME
                     PAIAMSPGLSHSPTITTPAMTQAGLILGTAAYMSPEQARGKPVDRRADIWAFGCVLYE
                     MLTGQRAFGGEDVTVTLARVVEREPDLDLLPPDTPARVRKAVRVCLRKDPRQRGGDIA
                     AIRLALEGAFETSAPQTSERVPIPRPLWRRAAPLAVVTTIALVLAFVAFRPNPLPEVM
                     RFQIHAPPGSTLPLGTPAVSPDGHALAYAVSDSTGVTRLHVRPIDRVETRVLPGTEGA
                     VHPFWSPDGRSLAFLPLPGRQLTVIDVEGGAPRVVAKEVGSPWSGTWNQNGDLLYWPA
                     AVTMRISAQGGTPTPVTSLDQQRGETQVLFPFFLPDGERFLVRVTSRDRSSIQLAILG
                     TMGRTMVVDDVDSAPILAPSPGGKTYLLYLRAPDLMAQEFDQTSGSVRGDPAVLVSNI
                     GQVGAPPVHPAVGVSPSGILAYQTGGVQIGRLTWVDRSGTEVEKLRAESTFFWNPQLS
                     PDGSRAAGFRFDPSSGATDVWVTDLQRASSTRVTFGNENESDAVWSPDSTRVAFRRRG
                     GTREPGIFVVDVNGGGNEQRLTTTPGTVTSWSPGGRYVLYDFQGRLTLLPVIGDEKPV
                     DVGSRNGRSRHGRFSPDGNYISFTSDETGRDEVWLQAMPPGKGSWKVSVNGGVEPRWR
                     KDSKEMFFVAPDGAVMAVAVRAAHALPVGVPQELFRQESFRLAGWTSTFGYDVRADGQ
                     WFLVSGLGEGADDAPITVVVNWWVELE"
     gene            complement(3581..4141)
                     /locus_tag="AC16CY_02645"
     CDS             complement(3581..4141)
                     /locus_tag="AC16CY_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3352705.1"
                     /translation="MTRRVVAVVSFALACTASLAAGQRSAPAAAPRPSPTAPPSLRPP
                     APAPAFRSGLILRGPADPLRPLSPFRGPGRGVLLPGFYYGWGWNFTPIYFDGSVVATP
                     LPADAPTGGLQLDVTPWRSRVYLDGEYVGQVENFKGYYKHLTAPAGPHQIVIVEDGYL
                     PLVLDVLIAPGQTTTYRGTLNEAPGR"
     gene            4196..5086
                     /locus_tag="AC16CY_02650"
     CDS             4196..5086
                     /locus_tag="AC16CY_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3352706.1"
                     /translation="MNRRAISFFVMTAAVTSASAAQSPSSPTTAPAFEVVSVKRNTTE
                     SGDSSISQPPDGSLRMVNVSVRTILGRAYQGSTLDSIGLPDWASSERYDVAATPVPGR
                     PNPSQDERRDMVRAMLADRFKLSAHYETREQPAFNLVLAHTDGRLGPGLKPSEVDCAA
                     RAAARRADAEAAKAAGQPPPPPVAVFSRDGAVPTCGFNFGFDRFEGDTTLASLAPMLR
                     SMAGRPVVDKTGLSGSYRVNFQFSRASPEPAGPDAPAPGDAPPSVFTALQEQLGLRLE
                     STRIPVEVLVIDRIERPTEN"
BASE COUNT          992 a         1930 c         1854 g          947 t
ORIGIN      
        1 cggttcgccg gcgcgctctt cgacggcatg cgggggatgg ggcgtctctg gcaggccgcg
       61 ggcacggtga atgccgtgct cgcgcccggc ctcctcgaga aggccgtaga agcaggacta
      121 cgcagcctct tcgtgggatt cgagacgctc aacccggaca acctcgtcga gcagcggaag
      181 tatcagaaca tgcgccgcga ctacgccgcg gcgatcacgc gcctccacga tctcggcgtg
      241 atgatcaacg ggagcttcgt cttcgggatg gatggcgacg acggctcggt gttcgagcgg
      301 accgtcgagt gggccatcga gcacgggatc gaaacggcca cgttccacat tctcacgccc
      361 tatccgggca cggcgctcta ccgccgcatc gcggaccagg gccgcctgac gaccggcgac
      421 tgggatctgt acgacacgcg gcacgccgtg ttccgccccg cacgcatgac ggccgatcag
      481 ctcgaatgcg gctaccactg ggcctaccgc gagttctacc ggtggcgttc gatcgcgcgc
      541 ggcgcgatgg cgcacgacga cctcctcgcg ggcctgcgtc atgcggcgta cgcggcgggc
      601 tggaagaagt tcgagccgct gtgggatttc gtgatccggg ccaaacgcgc cgggatgatg
      661 ctgccggttc tcgaaacgat cctcagcgag ttcggaaggc gcctacctgg ttcggcgact
      721 cgcggcgatc gacggaggac gacgtcggag gagcgagagc aggccgatca cgcgtccgtg
      781 ccgcagctcc tgcagattca gcccgcgatc tcgggctctt cggccagact ccaactcact
      841 ccagctccac ccaccagttg acaaccaccg tgatcggcgc gtcatcggcc ccttctccga
      901 gtccggatac cagaaaccac tggccgtcgg cgcggacgtc gtagccgaat gtcgaggtcc
      961 agcccgcgag ccggaacgac tcctgccgaa acaactcctg agggacgccg accgggagtg
     1021 catgggcggc tctcactgcc accgccatca cagcgccgtc cggggccacg aagaacatct
     1081 ccttgctgtc cttcctccag cgcggttcca ctcctccatt gacggagacc ttccagcttc
     1141 ccttgcccgg gggcatcgct tgcaaccaca cctcgtcgcg cccggtttca tcggacgtga
     1201 acgagatgta gttgccgtcc ggtgaaaacc ggccgtgacg tgaccgtccg ttgcgggacc
     1261 cgacgtcaac cggcttctcg tctccgatga cgggcagcag cgtcagcctg ccttggaagt
     1321 cgtagagtac ataccgaccc cccggggacc atgacgtgac cgtgcccggc gttgtcgtca
     1381 gtcgttgttc gtttccgccg ccattaacgt cgacgacgaa gatgccaggc tcgcgcgtgc
     1441 cgcctcgccg ccgaaacgcc acgcgtgtgc tgtcgggcga ccacacggca tcgctctcgt
     1501 tctcgttacc aaaggttact cgcgtggacg atgcccgttg caggtcggtg acccagacgt
     1561 ctgtggcgcc cgatgacgga tcgaaacgaa accccgccgc tcgggaccca tccggagaca
     1621 gctgcgggtt ccaaaagaac gtcgattccg ccctcagctt ctccacttca gtacccgatc
     1681 ggtcgaccca ggtcaatcgt ccgatctgaa ctccaccggt ctgatacgcg aggatgccgc
     1741 ttggcgaaac cccgacagca ggatgcaccg gcggggctcc cacctgtcca atgttgctca
     1801 cgagcactgc gggatcgcct cggacgctcc ccgacgtttg gtcgaactcc tgggccatca
     1861 ggtccggtgc gcgcaggtac aacaaatagg tctttcctcc gggcgacggg gccaggatgg
     1921 gcgcgctgtc gacatcgtcg acgaccatcg tgcgtcccat cgtgccgagg atcgcaagtt
     1981 gaatcgaaga cctgtctcgg gacgtgaccc gtaccaggaa tcgctccccg tcggggagaa
     2041 agaaaggaaa caggacttgg gtttcgcctc gctgctgatc caggctcgtg accggcgtgg
     2101 gggtcccgcc ctgggccgag atccgcatcg tgacggcggc cggccagtat aggaggtcgc
     2161 cgttctggtt ccaggttcct gaccaagggc tgccgacctc cttcgcaacc acgcgcggcg
     2221 ctccgccctc gacgtcgatc acagtgagct gacgaccggg cagtgggagg aaggcgagcg
     2281 accgcccatc aggagaccag aacgggtgca cagctccctc agtaccgggg agcacccggg
     2341 tctcgacgcg atcgatcggg cgcacgtgaa gccgcgtgac acctgtcgaa tcgctgaccg
     2401 cgtaggccag cgcgtgcccg tcaggcgaca cggctggtgt ccccagtggc agcgtgctcc
     2461 ctggcggcgc gtgaatctgg aaccgcatca cctccggcag tggattgggt cgaaaagcga
     2521 cgaaggccaa taccaaggcg atcgtggtga caaccgcaag tggcgccgct cggcgccaga
     2581 gagggcgcgg aatgggaacg cgttcgctcg tctgcggtgc cgacgtctcg aacgccccct
     2641 cgagcgccag ccggatggcc gcgatgtcgc cccctcgctg ccttggatcc ttccggaggc
     2701 agacgcgcac ggcctttcga actcgcgccg gcgtgtcagg gggcagcagg tcgaggtccg
     2761 gctcccgttc gacgacccgc gccagagtga ccgtgacgtc ctcgccacca aacgcccgct
     2821 gcccggtcag catctcatag agcacgcacc cgaaggccca gatatcggcg cgcctgtcaa
     2881 ccggtttgcc gcgggcctgc tcgggcgaca tgtaggccgc cgtgcccagg atgagcccgg
     2941 cctgcgtcat cgcgggcgtc gtgatcgtcg gtgagtgcga aagaccaggc gacatcgcga
     3001 tcgccggctc catcgccttc gccagcccga agtccagcac cttcaccgtt ccgtccggtc
     3061 gcagcttgat gttgcccggc ttgagatccc ggtggacgat cccccgctcg tgcgcggatt
     3121 caagggcttc agcaatctgc ttcgcaatcg cgagcgcttc ctcaatcggg acgggcccct
     3181 cggtaatgcg ttcggccaac gttgggccct cgacgagctc catcacgagg ccgttcatgc
     3241 ccgacgactt ctcgaagccg tggacgatgg cgatgttggg gtgattcagg gacgcgagcg
     3301 tcttcgcctc gcgctcgaag cgtgccagcc ggtccccgtc ctgtgcaaac gattcgggca
     3361 gtaccttgat cgccacctcg cgtccgaggt tggtgtcgat cgcacgatac acttccccca
     3421 tcccgcccac accgatttgg aaggtgattt cgtacgggcc aaggcgggtg ccctgagcga
     3481 gcgccacagc cgggattcta cgcgattgga actgttgtgc ggctcatgat gagggagccg
     3541 ttggcacgtt caggtgcgat cgttgcagtg gagacccacg ctaccggcct ggcgcttcgt
     3601 tcagcgtccc gcggtacgtc gtcgtctgtc cgggtgcgat cagaacgtcg aggacgagcg
     3661 gcaggtaccc gtcttcgacg atgacgatct ggtgcggccc ggctggcgcc gtgaggtgtt
     3721 tgtagtagcc cttgaagttc tccacctgtc ccacgtactc gccgtcgaga tacacgcgag
     3781 agcgccaggg cgtgacgtcg agctgaaggc cgcccgtggg cgcatcggcg ggcagcggcg
     3841 tcgccaccac cgatccgtcg aaatagatcg gcgtgaaatt ccagccccat ccgtaataga
     3901 accccggaag gagcactccc ctgcccgggc ctcgaaacgg agacagcggc cgcaacggat
     3961 cggccggacc ccggagaatc agccccgacc tgaaagccgg agcgggagcg ggcgggcgta
     4021 acgacggagg cgccgtgggc gatggacgag gtgcggcggc cggggcggag cgctggccgg
     4081 cggccaacga cgccgtgcag gccagtgcga acgacacgac tgcgacgacg cggcgtgtca
     4141 tatggatcac tgtacaccga tcggccgtat gatcggcggc atcgcgcatc ctccaatgaa
     4201 tcgacgggca atttcatttt tcgtcatgac cgcggcggtg acgagtgcat ccgcggctca
     4261 gtcgccctcg tcgcccacga cggcgcccgc cttcgaggtc gtgtcggtca aacgcaacac
     4321 gacggagtcg ggggacagca gcatcagcca gccgccggac ggcagcctca ggatggtcaa
     4381 cgtgtcggtg cggacgatcc tcgggcgggc ataccaggga tcgacactgg acagcatcgg
     4441 gcttcctgac tgggcctcat cggaacgcta cgatgtggcg gccacgccgg tgcctgggcg
     4501 gccgaacccg agtcaggacg aacggcgcga tatggtgcgc gcaatgctcg ccgaccgctt
     4561 caagctgtcc gcgcactacg aaacccgcga gcagccggcc ttcaacctcg tcctcgccca
     4621 cacggacggc aggctgggtc cggggctgaa gccttcggag gtcgactgcg cggcgcgcgc
     4681 agccgcgcgg cgggctgacg ccgaagccgc aaaggccgca ggccagccgc cgccgccgcc
     4741 cgtggccgtc ttttcaagag atggggcggt gccgacctgc ggcttcaatt tcggcttcga
     4801 ccgtttcgag ggagacacga cgctggcgtc gctcgccccg atgctgcgat cgatggcggg
     4861 acgtcctgtc gttgacaaga ccggcctgag cggctcgtat cgcgtcaact tccagttctc
     4921 tcgcgcctcg cctgaacccg cgggccctga cgcgcccgcg cccggcgacg cgccgccgtc
     4981 ggtcttcacg gcgctgcagg agcagctcgg actcaggctc gaatccacgc gcatccccgt
     5041 cgaagtgctc gtcatcgacc gcatcgagcg gccaacggag aactgaacgg cactagaacg
     5101 gagctcagtt gtgtatggaa cgtcgggcaa gcccgcgaga caacgcggaa aacacgtgct
     5161 tccggcactc ccgtcggcac acgtttatct tcctacctgg tggccacgcc ttcgccgcgc
     5221 ggcgagcctc cagcacgttt ttaccccttc gctgatcgcg tcgcctcgaa gggcttgccg
     5281 ccattccaca cacaactgag ctccggatat gggtccagaa cgcaatagag caggtccgga
     5341 gagctgactt gcgttgggca tggcgcaatc ccttatgtgg aggtggccga ctcgatgcgc
     5401 ctgaccgcgc gagaaggccc aggggatcag cgaggttgag cgccgctcga atcgcagctc
     5461 gttgcccgac gtacttcgtc cgtcgcgtag cgacgcaggg cgcgacccga gcgtcaggcg
     5521 gccgcgaatg ccatcgcagc gcggtccggc caccggaacc gggattcgcg atgaccaatg
     5581 caagccagct cgccggacat tgcctcttgc tctttgacct agcacttcac gtgcgcgaga
     5641 cgagttgtcg gactacgtaa ggaagaatcc cgccgtgctg gaaggcgacg agttcctctg
     5701 gtgtatcgat gcgcacggtc gcc
//
LOCUS       DBNGSH010000121         6122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_8663, whole genome shotgun sequence.
ACCESSION   DBNGSH010000121 DBNGSH010000000
VERSION     DBNGSH010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_8663"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="AC16CY_02655"
     CDS             complement(<1..1786)
                     /locus_tag="AC16CY_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3352707.1"
                     /translation="MSVAGLLPALSLLAGTALGLHIDLDPRAAAWAMPPLWVLSVVCW
                     TMRRPRLALALIAAGMACGGVALATGARDRALATSVRAELDPRFGGFLIEEPGPAGRH
                     DPVPSRAILVEDASLRDGFVSLRARLTAVSLDGAWRRVNGGITISVSGSAAPAAVDGW
                     RAGRAIEAPMTFRRPARYLNDGVPDFERELALDGVTLLATVKSALLVDVTAHGGMLQE
                     FAALVRAHVRRAVAAWIAPHDDVSGAIASAVLIGDRTGLPDETREALQAAGTYHVIAI
                     SGGNIAILASAATVFLLALGVRGRKASALTIAVLAAYALVVTTGPSVWRATLMAGLYF
                     GARALDHRTAAWHAASVSAALMLVASPLDVRDPGFILTFGATLALLEGARIGSAYTPR
                     ARAMSWLAASLTASLAVEAALLPVSATLFSRVTAAGLLLNLCAVPLMAVVQIGALVAA
                     LATSIPSIAAAAGWAAHLAAQGLVDSAGLVTFVPWSTARVPPPGVPMMCVYYAALFTV
                     LVSSGRAARVCGAAVLLLAALSVVGLVRLPAPGWPTDAERPLRLTVFDVGQGESMLVE
                     TPGGDRILVDTGGRPFGGGVDIGSRVLAP"
     gene            complement(1935..4328)
                     /locus_tag="AC16CY_02660"
     CDS             complement(1935..4328)
                     /locus_tag="AC16CY_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3352708.1"
                     /translation="MSRRSLSPAQSVVLTILLAAVPAAAQSRGASLSGDVVDESGARI
                     ATAVLVIRHESRGTELRLTTDNGEYSCGNLTPGEYTVTASAPGFAVTTLRVSLPSGGR
                     QTLRFELPVGALTEDIVVLATEVAGGHEALRRLPGSVEILEHDTLERSRVVTTNEALR
                     KVPGVHVRDEEGFGLRPNIGIRGINPTRSNKTLLLEDGIPLTYAPYGDNASYYHPPIE
                     RFERIEVMKGGAQIAYGPQTVGGLVNFVTPLPPTDPAAAITLTAGDRRYFNGHATYGV
                     TLGRSGVLFDYMRKQGDGARENVSSDLNDVTGKLVHTFSPGKAITLRGNYYSEDSTVT
                     YSGLRLAEYEADPRSNPFGNDSFSGDRYGASATPALTFANAALTANVYFSSFRRHWWR
                     QSSHSSQRPNDSGDPACGGMANLDTTCGNEGRLRQYSAWGVEPRLRVHHRMLGVTNET
                     DVGVRLHFERQDREQRNGSTPTARTGVLVEDNLRTNAAQAGFLQNRFLLGDWTVTPGV
                     RVEHVEYERTNHLANGGLGATGRTSLTRLVPGLGVSQATSERFTWFAGVHRGFAPPRT
                     EDVISNTGGVVDLDPELSWNYEVGGRSTLQPGIRLDGTVFVTDYENQIVPASLAGGVG
                     AALTNGGATLHRGFELMALLDSAPLLNSAHNLYVRAAYTALPVAQFVGVRFSNVPGFG
                     GVSVTGNRLPYAPEHLATIGAGYVHPSGVDAMIEAVYTSSQFGDDLNTVDSTSDGQRG
                     LIPGYMVWNTAVSYAFRRATAFVTVKNLLDELFIVDRARGILPGSPRLVQAGIRLRF"
     gene            complement(4463..5395)
                     /locus_tag="AC16CY_02665"
     CDS             complement(4463..5395)
                     /locus_tag="AC16CY_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3352709.1"
                     /translation="MTTRILESRTTIVFVVTLVAVFVCVVALAAQSFRGRIDIVQVTV
                     TVRDADGRLVTGLTKDDFQIFEDGREETVTQFTDERVPVSLGVLLDASDSMRGQAIDD
                     ARVAVDRFVGDLLLDEDEALVASFNHRPSLASTWTMPPWALRNALAGLKPSGGTAVYD
                     ALAEMADLYDHRNHLRAALVVISDGADTASDRSLTQTLEALRRSDALVYAIAIDAADA
                     RDSTRVNPEALKEVSGLTGGYTEVVRSAADLEPATARIAEELNKQYTLGYSSSRPPDG
                     TWRAIRVRIRTHDGYTARSRRGYYALPPAPSGRR"
     gene            complement(5448..>6122)
                     /gene="carB"
                     /locus_tag="AC16CY_02670"
     CDS             complement(5448..>6122)
                     /gene="carB"
                     /locus_tag="AC16CY_02670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF014225.7"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR3352710.1"
                     /translation="KATGVPMAKIAAQIAAGRTLAELGLVDDLEAAGVFVKSPVFPFV
                     RFPGVDTILGPEMKSTGEVMGGADNFGAAFGKAMMSAGQSLPERGCVFISVNNSDKQT
                     VLPIARDLSQLGFTLAATRGTAAFLRAHGLEVEVMYKVNEGRPNVADHLVNRSIDMVI
                     NTPLGRESFFDDRAVRRAATMAQVPCITTLTGASAAVSAIKALRQQTLTVRSLQDYYS
                     RVETGA"
BASE COUNT         1028 a         2092 c         2061 g          941 t
ORIGIN      
        1 ccggcgccag gacgcgagac ccgatgtcca ccccgccgcc gaacggcctg cctccggtat
       61 cgacgaggat gcgatcgccg cctggggttt cgaccagcat cgactcgccc tgcccgacgt
      121 cgaacacggt gaggcgcagc gggcgctccg cgtctgtcgg ccacccaggt gccggcaggc
      181 gcacgaggcc gacgaccgat agagccgcga gcagcaggac agcggctcca cagacgcgcg
      241 ccgcgcgccc tgacgagacc agcacggtga agagggcggc gtagtacacg cacatcatgg
      301 gaacgcccgg cggcggcacc cgcgccgtag accacggcac gaacgtgacg agtccggcgc
      361 tgtccacgag cccctgcgcc gccaggtgcg cggcccagcc ggctgccgcc gcgatcgacg
      421 ggatcgacgt cgccaacgct gcgacgagcg cgccgatctg cacgacagcc atcagcggaa
      481 ccgcgcacag gttgagcagc agcccggccg ccgtgacgcg tgagaagagc gtggccgaca
      541 ccggcagcag cgcggcttcg accgccaggg acgccgtcag cgacgccgcc agccacgaca
      601 tcgcgcgagc acgcggcgtg tatgcggagc cgatccgcgc tccctccagc agcgccagcg
      661 tggcgccgaa ggtgaggatg aagcctggat cgcgcacgtc cagcggcgac gccacgagca
      721 tgagcgcggc cgacaccgac gcggcgtgcc aggcggcggt gcgatggtcc agggcgcgcg
      781 ccccgaaata gagccccgcc atcagcgtgg cgcgccagac cgacggcccc gtcgtgacga
      841 cgagcgcgta ggccgcgaga acggcgatcg tcagggcaga cgctttccgt ccgcgaacgc
      901 cgagcgccag caggaacacc gtagcggcgc ttgcgagaat cgcgatgttg ccgcccgaga
      961 tcgcaatgac gtggtaggtg cctgccgcct gaagagcctc gcgcgtttcg tcgggcaacc
     1021 ccgtgcggtc cccgatcaac acggccgagg cgatcgcgcc agacacgtcg tcgtggggcg
     1081 caatccaggc tgcgacggca cgccggacgt gcgcgcgtac gagggcggcg aactcctgga
     1141 gcatgccgcc gtgcgccgtc acgtcgacga gcagggcgct cttgaccgtc gccagcagcg
     1201 tgacgccatc gagcgccagc tcgcgctcga aatcgggcac cccgtcgttc agatacctcg
     1261 ccgggcgccg aaacgtcatc ggcgcctcga tcgcgcggcc cgctcgccag ccatcaacgg
     1321 cagccggcgc ggccgatccg ctgacgctga tggtgatgcc gccgttcacc cgccgccacg
     1381 caccgtccag tgatactgcc gtgagccgtg cgcgaaggga cacgaatccg tcgcgcagcg
     1441 aggcgtcctc gacgaggatg gcacgcgacg gcaccgggtc atggcgcccc gcgggcccgg
     1501 gctcctcgat caggaacccg ccaaaccgcg gatccagctc agctcgcacg gacgttgcga
     1561 gcgcgcggtc gcgcgcgccg gtcgccagcg ccacaccgcc gcaggccatt cccgcagcga
     1621 tcaaggcgag cgcgagccga ggccgacgca tcgtccaaca gaccaccgat aaaacccaga
     1681 gcggcggcat cgcccaggcg gccgccctgg ggtcgaggtc gatgtgcagg ccaagcgcgg
     1741 ttcccgcaag aagggaaaga gccggaagca ggccggcgac ggacacgccg gggcaatgca
     1801 agacgcggcc gcggccggcc gccgcgtctt atcaaggact tagagacctc gcgttgaaga
     1861 ttcttctctg ccgcgagagg gctcctgcgg cagtttctgc cactcacgca gaacgggcac
     1921 gtgcggcatt gcgcttagaa ccggagccga attcccgcct gtaccaaccg cgggctcccg
     1981 ggcagaatcc cgcgggcccg gtcgacgatg aacagctcgt cgaggaggtt cttcaccgtg
     2041 acgaatgccg tggcgcggcg gaaggcgtag ctcacagcag tgttccagac catatagccc
     2101 gggatgagtc cgcgctggcc gtcagaggtc gagtcgacgg tgttgaggtc gtcgccgaac
     2161 tggctgctcg tgtagaccgc ctcgatcatg gcgtccacac ctgacggatg cacatagccg
     2221 gcgccaatgg tggcgaggtg ctcgggcgcg tacggtaaac ggttgcccgt gacactgacg
     2281 ccgccgaaac cgggcacgtt gctgaatcgg acaccgacga actgtgccac tggcaaggcg
     2341 gtataggcgg cccgaacgta cagattgtgc gcagagttca acagcggcgc ggagtccagg
     2401 agagccatga gctcgaagcc ccggtgcaga gtggcgccac cgttcgtgag ggccgccccc
     2461 acgccaccgg cgaggcttgc aggaacgatc tggttctcgt agtcggtgac gaacacggtc
     2521 ccgtccagcc tgatgcccgg ttgcagtgtg ctccggcctc caacttcgta gttccagctc
     2581 agctccggat caaggtcgac cacgccaccg gtgttggaga tgacgtcctc ggtgcgcggc
     2641 ggcgcaaacc cccggtggac accagcgaac cacgtgaacc gctcactcgt tgcctgcgac
     2701 actccgagcc ccgggacgag ccgagtcagg ctcgtccgac ccgtcgctcc caagccgccg
     2761 ttcgccaggt gattcgtgcg ttcgtactcg acatgctcca ctcgcacacc tggtgtcacc
     2821 gtccagtcgc cgagcaggaa ccggttctgc agaaagcccg cctgcgccgc attcgtgcgg
     2881 aggttgtcct cgacgaggac gcccgtgcgg gctgtcggcg tgctgccgtt cctctgctcg
     2941 cggtcctgtc gctcgaagtg caggcggacg ccgacgtccg tttcgttcgt cacgccaagc
     3001 atgcgatggt gcactcgcag ccgaggttcg actccccatg ccgagtactg gcggagccga
     3061 ccttcattgc cgcaggtcgt gtcgaggttt gccattccgc cgcacgccgg atcgcccgag
     3121 tcgttcggac gctgcgagga gtggctcgac tggcgccacc aatgccggcg gaacgaggag
     3181 aagtagacgt tggccgtgag cgcggcgttg gcgaacgtca gcgccggggt tgccgacgcg
     3241 ccatagcggt caccggagaa ggagtcgttc ccaaacggat tgctccgcgg atcggcctcg
     3301 tactcggcga gtcgcaggcc cgagtacgtg accgtggagt cttcgctgta gtaattaccg
     3361 cgaagcgtga tcgccttgcc gggcgagaag gtgtgcacga gcttgcccgt gacgtcgttg
     3421 agatcggagc tcacgttctc tctcgcgccg tccccctgct tgcgcatgta gtcgaacaga
     3481 acgccggatc gaccgagcgt gactccgtaa gtggcgtggc cattgaagta ccgccggtcc
     3541 cccgcggtga gcgtaatcgc cgcagcggga tcggtcggcg gcaggggcgt cacgaaattg
     3601 accaggcccc cgaccgtctg tggcccgtag gcgatctgcg caccgccctt catcacctcg
     3661 attcgctcga agcgttcgat gggagggtgg tagtacgacg cgttatcgcc gtacggcgcg
     3721 taggtcagcg gaatgccgtc ttcgagcagc agcgtcttgt tcgatcgcgt cgggttgatg
     3781 ccgcggatgc cgatattggg ccgcaggccg aagccttctt cgtctcgcac atgcacgccc
     3841 ggtactttgc gcagcgcctc gttggtggtg acgacgcggc tgcgctcgag cgtgtcgtgt
     3901 tcgagaatct ccaccgatcc cggcagccgc cgcaacgctt catgaccgcc cgcgacttcc
     3961 gtcgcgagga ccacgatatc ctcggtgagc gcaccgacgg gcagctcgaa ccgtagcgtc
     4021 tgccggccgc cggaaggaag cgacacgcgt agcgtcgtga cggcaaagcc tggggcgctg
     4081 gcagtgaccg tgtactcgcc cggcgtcaga ttcccgcagg aatactcgcc gttgtcggtc
     4141 gtcagtcgca gttcggtccc gcgtgactcg tgtcggatga cgaggaccgc cgtcgcgatt
     4201 ctggcgcccg attcgtcgac gacgtccccc gagagcgagg ctccccgact ctgggccgcg
     4261 gcgggtacag ctgcgagcag gatcgtcaag acgacagact gggcgggcga gagcgaccga
     4321 cgagacatca gggttccctc catgggagcg gcggcggcac taagtatcga tactaagact
     4381 tagtctcact atcaatatgg caagggtata tccgaccgtt ccagtccgtc aagcgcctct
     4441 tcaaggggtt gcgagccgga cgtcagcggc ggcccgaagg agccggcggg agggcgtagt
     4501 agccgcggcg cgatctcgcg gtgtagccgt cgtgtgtgcg aatgcgaacc cggatcgcgc
     4561 gccacgtccc atcgggcggg cgcgacgagc tgtaacccag cgtgtactgc ttgttcaact
     4621 cctcggcaat tcgcgccgtg gccggttcca ggtccgcggc cgatcgcacg acctcggtgt
     4681 acccgccggt caacccgctg acttccttga gcgcctccgg gttcacgcgg gtgctgtcgc
     4741 gtgcatcggc cgcgtcgatc gcgatggcgt acacgagggc gtcgctccgg cgcaacgcct
     4801 cgagcgtctg cgtcagcgac cgatcgctcg ccgtgtcggc gccgtccgag atgacgacga
     4861 gcgcggcgcg caggtggttg cgatggtcgt agagatcggc catctcggcg agggcgtcgt
     4921 acaccgcggt gccccctgag ggcttcaatc ctgccagcgc gttgcggagc gcccacggcg
     4981 gcatcgtcca cgtcgatgcc aggctcggac gatggttgaa gctcgcgacg agggcttcgt
     5041 cctcgtcgag cagcaggtcg cccacgaagc gatcgacggc gacgcgcgca tcgtcgatcg
     5101 cctgtccgcg catgctgtcg ctggcgtcga ggagcacacc caggctcacc gggacgcgct
     5161 cgtcggtgaa ctgcgtgacg gtctcttccc tgccgtcctc gaagatctgg aaatcgtcct
     5221 tcgtgaggcc ggtcaccagc cggccgtcgg cgtccctcac cgtgaccgtc acctggacga
     5281 tatcgatgcg cccgcggaag gactgcgcgg cgagagccac gacgcacacg aagacggcca
     5341 ccaaggtcac gacgaacacg atggtcgttc gcgattcgag gatgcgcgtc gtcatgaacc
     5401 gatcttccgg tgtttcgaga gccctcgcga ccgtcgtgcc ttcgtggcta cgcgcctgtc
     5461 tcgaccctgc tgtagtagtc ctgcagtgaa cgcaccgtga gcgtctgctg ccgcagggcc
     5521 ttgatcgcgc tgacggccgc cgacgcgccc gtgagcgtcg tgatgcaggg cacctgtgcc
     5581 atcgtcgccg cccggcggac ggcgcgatcg tcgaagaatg actcgcgtcc gagcggcgtg
     5641 ttgatcacca tgtcgatgct gcgattgacg agatgatcgg cgacgttggg ccggccctca
     5701 ttgaccttgt acatgacctc gacttccaag ccatgcgcac gcaggaacgc cgcggtgcca
     5761 cgcgtcgccg ccagcgtgaa gcccagctgc gacaggtccc tggcgatggg aagcacggtc
     5821 tgcttgtcgc tgttgttgac gctgatgaac acgcagcccc tctcgggcag actctggccc
     5881 gcgctcatca tcgccttgcc gaacgcggcg ccgaagttgt cggccccgcc catcacttcg
     5941 ccggtggact tcatctccgg gccgaggatc gtatccacgc cgggaaatcg cacgaacggg
     6001 aacaccgggc tcttgacgaa cacgcccgcc gcctcgaggt cgtcgacgag acccagctcg
     6061 gccagcgtgc ggccggccgc aatctgtgcg gcaatcttgg ccatcggcac gcccgtcgcc
     6121 tt
//
LOCUS       DBNGSH010000122        23783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1027087, whole genome shotgun sequence.
ACCESSION   DBNGSH010000122 DBNGSH010000000
VERSION     DBNGSH010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 23783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23783
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1027087"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..554)
                     /locus_tag="AC16CY_02675"
     CDS             complement(72..554)
                     /locus_tag="AC16CY_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352711.1"
                     /translation="MNRARWVALVVVAASSLAWYSMMPPAPVVLPPADAAGATPVRGV
                     IHVHTRRSDGAGTIDDVAAAAARAGLQFVILSDHGDGTGMREPPSYRSGVLCIDAVEI
                     RTSGAPDETRDRRWRRSVYLDDMEQTITVFLDEMTPVGDRSGSWSCRPYVTSSSWSIR
                     "
     gene            complement(551..1450)
                     /gene="xerD"
                     /locus_tag="AC16CY_02680"
     CDS             complement(551..1450)
                     /gene="xerD"
                     /locus_tag="AC16CY_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR3352712.1"
                     /translation="MPKSVPALDAYLTHLTVERRLAANSIESYARDLNDLAEFADARA
                     RRVHELSRQDLEALVRRWMSEGRSPRSVARAVACYRGFYRFLLVDGRVRANPAEDLHA
                     PRAWKVLPRYLSVEDVDRLIAQPDVKTPRGLRDRAVIELMYATGMRVSELVTLRPADV
                     HLEASYLTCTGKGDKQRIIPIGDEASAWVSRYLQQSRSILLRRRRSPRLFVNARGGGV
                     GLTRVGFWKILKGYARQAGITSTLSPHMLRHSFATHLLERGADLRAIQMMLGHADLST
                     TQIYTHVLEQRLRSVYDQFHPRA"
     gene            1523..2545
                     /gene="murQ"
                     /locus_tag="AC16CY_02685"
     CDS             1523..2545
                     /gene="murQ"
                     /locus_tag="AC16CY_02685"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIR3352713.1"
                     /translation="MPVRSKWQSLPTEAINAVSLGIDKLAADDIVELMLNEDRHMLDA
                     VRRERERIAVGVEMIAAALRKGGRIVFVGAGTSGRLGILESAEMPPTFGTNPDDIQAI
                     MAGGREAIFAAREGVEDHYEEGARAIARLRPTKKDIVIGVSASGMTQFVRGALTSARK
                     AGARIIFVTCDPGTELQTFVDLTIAPGVGAEVIAGSTRLKAGTATKLVLNMLTTGAMI
                     RTGKTYGNLMVDVQTGSEKLKDRARRIVNIVTGLEYEDADRLLKVAQWDVKAAIVMQK
                     TGLPYTKALSRLRRAHDSMREALGEDIEPRLRGLLQGHAEAAAVANEFSSMASGSAQT
                     RRRPKS"
     gene            complement(2540..3262)
                     /locus_tag="AC16CY_02690"
     CDS             complement(2540..3262)
                     /locus_tag="AC16CY_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR3352714.1"
                     /translation="MDIRILETSEVGRQVARRVAEALRQTPDLVLGLPTGRTPLDVYA
                     ELRRLYAAGEADFSRATAFLIDEFVGLDGSDTGSFRRQLGEQLLSVVNFDQARVHSLR
                     GLADDDAECRRYEDAIVAAGGLGLLLLGIGANGHIGFNEPGRTLVSRTHRVALLESTR
                     RDNAKLFGGDLTRVPAEALSMGMATILRAEAILLIATGQHKAAVVERMIRGPLSTQLP
                     ASFLQLHRHVEVYLDPAAASAL"
     gene            complement(3269..4204)
                     /locus_tag="AC16CY_02695"
     CDS             complement(3269..4204)
                     /locus_tag="AC16CY_02695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HIR3352715.1"
                     /translation="MHVLGIDAGGTKTVCLLADERGRVLAEAHGGGANLQTAGELETE
                     KVLHRVMEQALAQHDVRPDAICVGIAGADRPGDVKAVLDIMRRIGLKTRVVVVSDALV
                     ALVAGAGDQPGVVIVAGTGSIAYGRDAAGRAARAGGWGYLLGDEGGGFWIGRAALSAV
                     VRAFDGRAPATRLTDLVLKEMQLGTPVELIDAVYDRGLPGPAVAGLADLVQQAALAGD
                     AVAAEILSRAAGELVAAAASVVTRLGMRGDVFPTVLSGGIFSAIPALAADVTTRWAEV
                     APRSEVRPLDIEPACGAVVLALAEARGTLRLPTYI"
     gene            4240..5301
                     /locus_tag="AC16CY_02700"
     CDS             4240..5301
                     /locus_tag="AC16CY_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014675.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HIR3352716.1"
                     /translation="MASGVSRLVCLHARSAIWIGVVSVIAAAHAVASAADNSPARAFR
                     IPSAAMTSPAAMRRAISAAVAGSFDTVVVPVSATPLSDANLADSEAELVRQARENGLR
                     VHLAIAVNLAVATGELPSAREHVIYQHPEWLMVPRDAAVELLTVDARSPAYLGRLLRW
                     TRANADRVSGIYVSPLDPDAASYLVNAVTSALARHAADGVYLEALDFPGEDFDYSRRA
                     LDLFRARTRNALSPAERARVDEIEAIDPFAYEEEFPDAWRTFRESALTRLLERLRSTL
                     VAANPSLGVTVSASADPDVSLRHHFQNWRAWLAGRIVDRIGYPGPSSGVVLFSAEGAA
                     PADSSPLTSAQTAVGDGSR"
     gene            5309..6367
                     /locus_tag="AC16CY_02705"
     CDS             5309..6367
                     /locus_tag="AC16CY_02705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3352717.1"
                     /translation="MAPPFAAARAVIERAVADRAFPAAVVEVGTAHRPLWCEAFGRLS
                     FDTGARPAQTDTVFDLASLTKVLATVPLVMRAVERGNLGLDDLVSSHVPSWRDDGTAS
                     PVTLRDLLAHCSGLPAYAPLFKEHSGRAGFESAICTMPREYAPRSTAVYSDLGFILLG
                     FILEDIAPLPVQFDTIARQMGGMPDIQFHPPEIWRVRTAPARDDDWRGRVLLGEVDDN
                     NAWALGGAAGHAGLFGTAGAVGECTRHVLQVLEGRTGAFRKDTLLTFVTRRTDVPGSS
                     RALGWDTMLPTSSCGARLSPRAFGHTGFTGTSLWIDPERSVYVVLLTNRVHPTTANEA
                     IKAVRRSLHDAVIEDLDR"
     gene            complement(6468..7205)
                     /locus_tag="AC16CY_02710"
     CDS             complement(6468..7205)
                     /locus_tag="AC16CY_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3352718.1"
                     /translation="MKHGRAITSERGYAMAALLVAISVMAIAISTALPVFQTVARREK
                     EAELVFRGEQYARAIGMFQGRYGNALPPDVDTLIRDRFLRKKYKDPITGGDFLILGPG
                     SPELAQALNTPPQVPGRAGRGAQTTGRGAAQPARGGARGSTQTQQPTQPFAVSRFTQP
                     GAASGQAPGGVLAVASKSTQTSIRLYNGKDKYNEWVFMAQQRSTAAGAPPGVGGQTPG
                     VRGGAAPGGRRGATPAPARGGRGRGGL"
     gene            complement(7205..8164)
                     /locus_tag="AC16CY_02715"
     CDS             complement(7205..8164)
                     /locus_tag="AC16CY_02715"
                     /inference="COORDINATES: protein motif:HMM:NF040138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR3352719.1"
                     /translation="MPSRVYVGLWIALAGAAAACDTVQLLAPTGSTVTLTAASVVVPA
                     GGTTQVTAFVYESSGTPVQNGTTVRFSTNLGRVDPVEAQTRNGYAVATFSAGDVSGIA
                     DVSAWSGAAGGATGEGANGSATNVVQISVGSASVETVLLGANPGSLPAAGGTVELLAT
                     VLGTNGREMSNVFVLFASTEGQLASTRVATDAFGQARTTLTTTQTAKVTASAGLKTAE
                     TTVTRRDPVVPGLTAAPGAIQIGIGQTWNFTATLTPPNDPSAQAAAYDWDFGDGSTAT
                     TNSNVTSHVYTSGANTTRVVTVTIRLTNGQALVATTQILLGSF"
     gene            complement(8167..8511)
                     /locus_tag="AC16CY_02720"
     CDS             complement(8167..8511)
                     /locus_tag="AC16CY_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR3352720.1"
                     /translation="MTLVLILASVSLVVYGNNVQRAKEAALTENLWQMRKAIDEYYAD
                     KGKYPASLDALVTDKYLRSIPKDITGNVETWQEIPAEFDPGNPNAEPGIENVKSGSDQ
                     VSIDNTPYAEFE"
     gene            complement(8588..9055)
                     /locus_tag="AC16CY_02725"
     CDS             complement(8588..9055)
                     /locus_tag="AC16CY_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3352721.1"
                     /translation="MTNQRGFTFLELVIVTTILLILSSTVLPLSRVTAQRQREVELRR
                     DLREMRTAIDRFKDAYDRGQIARSEVEAGSEGYPPDLETLVDGVTVANDATGRKLKFL
                     RRIPQDPMTKSTDWGLRSYQDEPDSSSWGGENVFDVYTTSAGTALDGTKYRDW"
     gene            complement(9056..11422)
                     /locus_tag="AC16CY_02730"
     CDS             complement(9056..11422)
                     /locus_tag="AC16CY_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR3352722.1"
                     /translation="MRHSHAAALLVVVALFAGGCAAGNAFRRGDEAARGGDWDAAVVH
                     YREAVQADPQNPAYKIALERSMISASIGHLDKARLAEARGQLDEALREYRRASEYDPP
                     NRAVAQKVLDLERTIRDQIEAAKRPSPQQMREAARAGAAPPLFNFSTIVQPMKFQQAS
                     LRDILNSIGEGTGINVTYESTYQDRVYTVAMSDVTLEQALQQITQANQLFYKVMNPKT
                     IMIIPDTAPKRTQYEDQVVVTFPLSYGDPSEIMQSLQTVLRAAGQQLAPQFAPNKTNN
                     SITVRGTTAMMGIVERLIDILDKPRAEIVIDVQILEVNKGRVRQYGLDLGSYSINAVF
                     SPEADIRAVVTPPTNGDQNQDNATLSRAFNANTITRGISANDFYLAVPSAVIRFLETD
                     SETKVIAKPQLRGAEGSDIRMNLGEDVPVPSTTFTPLAQGGANFNPLTSFEYRSVGVI
                     VEMNPRVSLEGDIQMKLKLETSSIGPGTKIAGQELPSFNSRKVETDIRLREGESTLLA
                     GLLQESERKVLSGLPWLLNLPIIKQLFSSNDEQIQQSDIVMLLTPRIVRTRELTPQDL
                     APVYIGTQSNFGLSGPPPLVAPVAGGPPVPDATPGTVAGPAPQTPAVTAQAPAPQTPA
                     PPAGAPGTTAGAQPLGASKPGPATASAGQVLMSVPGPEFRLGGGPYLVPLSITGASQL
                     SGITLTVSYNPAVLRLVNVQEGSFMRAGGVSASFSQQADPAGGRVDIAIVRRADPTGV
                     AGTGLLAGLLFEAVASGAANLGVTGSATGPGGSAVNLQFVPVPAVRVP"
     gene            complement(11419..11955)
                     /locus_tag="AC16CY_02735"
     CDS             complement(11419..11955)
                     /locus_tag="AC16CY_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352723.1"
                     /translation="MTKTRLQLLVLGALVLAGGLLVYRQWPTSDVTGAGSTAPGTTGA
                     PAAGAESAVGDLRLELLEERGDDRVKLGRDLFRFHARPAPAASRQQVTLAPPVPTGPP
                     QPPPPPPIPLRFIGYVDQANRPPRVAVLSDGRGNVFHGREGDIIEGRYRVLRVGVDSA
                     DLMYVDGRGRQTIRLSGQ"
     gene            complement(11955..12536)
                     /gene="pilO"
                     /locus_tag="AC16CY_02740"
     CDS             complement(11955..12536)
                     /gene="pilO"
                     /locus_tag="AC16CY_02740"
                     /inference="COORDINATES: protein motif:HMM:NF022201.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HIR3352724.1"
                     /translation="MSDAMTLLRRILAERRRTVYALAGGVVVNVLVFAFLVYPLRAAV
                     ANVEQRTQSAEQAEAAAKGEFTTANGLLTGKDRAQKELNTFYTSVLAPDLAGARRLTY
                     ARLARLAEDASLDYEHGTYEPVSERGSNLTKFAVTMDLTGDYSDIRNFIHEIETAPEF
                     VVIDNVSLSEGTAGDGSLRLKLELSTYFWTGGL"
     gene            complement(12529..13131)
                     /locus_tag="AC16CY_02745"
     CDS             complement(12529..13131)
                     /locus_tag="AC16CY_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR3352725.1"
                     /translation="MLRTNLSTRPFYNLRAVQVTIGALAVIVAVMTLGNIVELVRLSA
                     SERDLGARAARAEEEAARLRVDARRIRGQINTRELTEVAEAAQEANAIIDLRTFSWSD
                     LFAQLEAALPDDVRLTGFKSRVDTEGRFVVSVPVLARRVRDLETFLDALEKSDRFDAV
                     LAAEERTNLEGLIDGLIEGVYIQPPRPAPPAPVTTGAAGE"
     gene            complement(13131..14096)
                     /gene="pilM"
                     /locus_tag="AC16CY_02750"
     CDS             complement(13131..14096)
                     /gene="pilM"
                     /locus_tag="AC16CY_02750"
                     /inference="COORDINATES: protein motif:HMM:NF022554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HIR3352726.1"
                     /translation="MSLLTSLLATPPLEAAIEISASAVSIAVLNGRVGVNAVVQGYAV
                     EPLPAGAVSPSLTAPNIVDRTAVVRALHAACQSIGVKPSRVALVIPDVTTKVSLVRFE
                     QMPARREDLTQLLRWQVKKSTPFPVEDACLTYTLGGQAAGAHEFVVVVARRDIIREYE
                     GACEDAGIEAGLVDTSTFSVINLFLSAGFGTSGDWLVVHMRPEYTSMAILRREDMMFF
                     RSRAEGDPDALEDVVHQTAMYYQDRLEGRGFSRILLGGIGRAPGAVDLARRNLEERMG
                     TIVEPIDPTCGAALTDRIHATPELMATLAPLVGMLLRSQREGVAA"
     gene            complement(14093..15718)
                     /locus_tag="AC16CY_02755"
     CDS             complement(14093..15718)
                     /locus_tag="AC16CY_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3352727.1"
                     /translation="MATVKTNPLPVPDPDLPEEVTRAQALAARYRLEFVDMKEFRIDQ
                     DLFRSIPADLMLRYGFVPHRREGKALVIVVSDPTDLPMIDELGVILRTPIRVTVGVPS
                     AIQSILKKSESSQRVLEEASEGFELQVLKEDDTGEESLTVERLTSDISPIIKLVDSTI
                     YTAIQRRASDIHIETGDDAVHVKYRIDGVLQPAMRPIGKQFHSSIISRVKVMAELDIA
                     EKRVPQDGRFKVRMPGKTIDFRVSIMPSVHGEDAVIRILDKESISEKFTELKLDILGF
                     PEQELRRFRKYIREPYGMVLVTGPTGSGKTTTLYAALSEIKSVEDKIVTIEDPVEYQL
                     RGITQIPINEKKGLTFARGLRSILRHDPDKIMVGEIRDPETAQIAIQSALTGHLVFTT
                     VHANNVVDVLGRFLNMGVEPYQFVSALNCVMAQRLVRLICTHCRRPARVDAKALNEAG
                     LPEELAQTGRFYEGAGCVECGGTGFKGRTAICELLDLSDRIREMILERRPASDIKKAA
                     HEEGMRFLRESAVEKVFTGLTTLREINKVTFVE"
     gene            complement(15718..16920)
                     /locus_tag="AC16CY_02760"
     CDS             complement(15718..16920)
                     /locus_tag="AC16CY_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3352728.1"
                     /translation="MEFRCRLGTPAGEIVEGLYVADSEALLRRELEEKGLYVLSLRRA
                     GLSLSFGRGRQRIKSHEFLVFNQELATLLKAGMPLVQSLDILRQRVPNRTFKTVLDSV
                     HDKVKGGVALSDAFAEHQSLFPPVYSASLVAGERSGNLDTVIRRYVSYEKIITAVRRR
                     TISAMIYPAILIAMMIVLVSIIVLKVVPAFSEFYANFEAPLPLLTRVIVTFSNTITNH
                     IGLILGGVVALVVLSMSWLRLPEQRERMHRWLLDVPWVGTTALKFYMSQLGRTLSTLL
                     GGGIPLVNALEISVRAISNRHLARELDTVRVRVQEGQGFAAALRERAAFPDVAVKMIE
                     VGESTGALQEMLNSLADFYDEEVDTEVGRFITMIEPALLVGMGVIIAGVVLALYMPLF
                     ELSSLVSL"
     gene            complement(16946..17824)
                     /locus_tag="AC16CY_02765"
     CDS             complement(16946..17824)
                     /locus_tag="AC16CY_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352729.1"
                     /translation="MPRYSVCTVLVAGLIAGCSGPELDAAKPVVTPSFSLSSPKVTAG
                     RPFEFALRFAVSPDAPAFTADYVVFVHFIDDKGKMIGAADHPPPTPTREWKAGSTVEY
                     SHTAFPPTADYRGDMKVVVGLYLPSSNERVPIAGELFEPRAVTVGSLTLTDLDDPYPV
                     SFREGWHSPESVKTSGVQWRWSMKSGRLSFPNPKRDVVVVIQADQPAPAFVEEPQTIQ
                     VKLGDSVVDTFRLATGPAELRRISLTQSQLGDGQTVDLAVVSDKTFVPAKLEALKSGD
                     TRQLGVRVFRAYVEPK"
     gene            17851..18126
                     /locus_tag="AC16CY_02770"
     CDS             17851..18126
                     /locus_tag="AC16CY_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR3352730.1"
                     /translation="MSSDALGMVETRGLIGSVEAADAMVKTANVVLVGKEYIGAGYVT
                     VLVRGDVGAVKAATDAGAAAARRVGELVSVHVIPRPHTEVERILPKP"
     gene            18148..19881
                     /locus_tag="AC16CY_02775"
     CDS             18148..19881
                     /locus_tag="AC16CY_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3352731.1"
                     /translation="MAVQDAAPHTDKDLASIAESRALARAARQAQPLLAEFSQEQIDS
                     IVTAMADAATREAEALAQLAVDETGYGVVADKVQKNLFSSRQVYEFIKPLKTVGVVNR
                     IDDRKIVEIAEPFGVVAAIVPSTNPTSTAIYKVLIALKARCPIVISPHPSAARCITRT
                     VEIMSEAASRAGAPAGSIGWMKTVTLEGTQELMKHRDVAVILATGGMGLVRAAYSAGK
                     PAYGVGPGNAPCYIERSADLGKAARDIVLGKTFDNGVLCSSPNSVVVDESVAAEARRQ
                     FEAQGAYFMNDAESDALAKQLVTPQRLPNPALVGKSAALIAEKVGLKVPAGTTVLIAP
                     LMGVGRDYPLSIEKLCPILSWYVVADWRAGCERCIQILRYGGMGHTMSIHSKNEEVIL
                     EFGLKKPAFRICVNTPTTHGSIGLTTGLDPAMTLGCGGWGGNITSDNISPRHLLNIKR
                     LAYELRPATRATADVGSAAAKGGQSLPAAPARRPIEGIPAEALSSRIDAFLSSRGFRP
                     AQQREAAATGAKKNEPVADTPEAFICEDDVRAAMQSGRKLLVGERTIVTPAARDLGES
                     ARVFVNAGWPH"
     gene            19968..21551
                     /locus_tag="AC16CY_02780"
     CDS             19968..21551
                     /locus_tag="AC16CY_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3352732.1"
                     /translation="MLTPSRALTVVCVLGVLATGCGKRFAPAGVSEIPSAEAVPSPAA
                     VTPPLDAEPAPSPEPVTPAQQAQAEPSETKEAEAQADPNFKEKAPEPAPIDELLAIST
                     VETPPPTPKTEKSVADDLQTIVHDIDIPQNARVLRYVELFSGKLKGYLEEGLGRGAQY
                     LPMIQDVFRAEGLPLDLAYVPLIESAFKPTALSRAKAKGIWQFMRGTALENGLTHDWY
                     IDERADPEKATRAAARYLKTLHGMFDDWHLALASYNGGPGRIQRAMKRSGRDDFWKLS
                     ANSRYLPRETRDYVPLILAAIVVARNPGHYGLNIAPVDARPFDTVSVAAAVDLRRVAE
                     WAEVTVAELQDLNPELRRWTTPVRATVYELKVPQGTADAINGRLALLADEGAPFTRYS
                     VKKGETIQGIAKKLKVTRSDLAEANYLSTKARLSTGQQLIVPRAPALTARASSPADRP
                     QPAATPDVVLASRSIGVEKAERPAPASTMHRVKRGETLFSIAKRYGTTVTLLKEWNGL
                     RGNVIQIGQRLIVGAYSTN"
     gene            21696..23231
                     /locus_tag="AC16CY_02785"
     CDS             21696..23231
                     /locus_tag="AC16CY_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR3352733.1"
                     /translation="MLACLRSAAVFGVEAYPVHIEVDVAFGLPHFSMVGLPDATVRES
                     RDRVRSAIRNSGFDFPPHRITVNLAPADVRKAGSSFDLPIALGLLATSGPLQRRAVDD
                     TVIVGELSLDGAINGIRGVLPIAVGARRLGVKRLLLPPQNAAEASVVEGLDVCIARSL
                     PEAVDALNRPDEAQRATSEPAAAVLPHVNDGDLSEVRGQLLARRALEIAAAGGHNLLL
                     TGPPGAGKTMMARRLGTILPPLTFDEALECTAIHSVAGTLPSGTGLLVDRPFRAPHHT
                     ISHVALVGGGAIPRPGEISLAHHGVLFLDEMPEFDRRVLEVLRQPLEEGRVSIARAAR
                     TAVFPARFVMIGAMNPCPCGFAGDERRPCRCTPVQVAKYRNRLSGPLRDRIDLHVEVP
                     AVPVGVITDTAAGEPSASVRERVLSARRLQHTRYGPDGPRTNAALAGSRITAICRPDA
                     KGRQLLRRAVDQLGLSARGHDRVLKVARTVADLSGADEVGCDHVAEALQYRLVEHGLG
                     AEA"
     gene            23239..>23783
                     /locus_tag="AC16CY_02790"
     CDS             23239..>23783
                     /locus_tag="AC16CY_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352734.1"
                     /translation="MNVVVGRREFLGSAAIVGVGSMTAGPLLVEGAGKHSHHTGGHIE
                     QELVDQLRTSLRGMQGARPAEGARQLASTLRLAAAHFKEKEIDADLKAFLHSTIRNEG
                     RESLLRRQIDKRMLAAEMQKLGVTAPLDLGPVDHAMREKALDMLLTTGLTPLMTRVAD
                     TLDRRSETLDRRETIRLSSAQ"
BASE COUNT         3907 a         7979 c         7886 g         4011 t
ORIGIN      
        1 tctacctcgt gctcaccgtg cgaacatgat ctcgtcccgc cacaccggac cgctcgaccc
       61 ggaccggctg ttcaccgaat cgaccacgaa gaggaggtca cgtacggccg gcagctccag
      121 gatcctgacc tgtccccgac aggggtcatc tcgtcgagga agacggtgat cgtctgctcc
      181 atgtcgtcca ggtacaccga cctgcgccag cgacggtctc gggtctcgtc gggcgctcca
      241 ctggtgcgga tctccacggc gtcgatgcag agaacaccgc ttcggtagga gggcggctcg
      301 cgcatccccg tcccgtcgcc gtggtcgctc aggatcacga actgaaggcc ggcgcgcgcg
      361 gcagccgccg ccacgtcgtc gatcgtgccg gctccgtccg atcggcgcgt gtgcacgtgg
      421 atcacgcctc gaaccggcgt cgcgccggca gcgtcagccg ggggcaggac caccggggcc
      481 ggaggcatca tcgagtacca cgcgaggctg ctcgcggcca cgacgacgag ggcgacccat
      541 cgagcccggt tcatgcccgc ggatggaact ggtcgtagac cgaccgcagc cgctgctcga
      601 gcacgtgcgt gtagatctgc gttgtcgaga gatcggcgtg ccccagcatc atctgaatgg
      661 cacgaagatc ggctccgcgc tcgagcaggt gggtggcaaa cgagtgccgc agcatatgcg
      721 gactcaacgt ggacgtgatg ccggcctgtc tcgcgtagcc tttcaggatc ttccagaacc
      781 ccacgcgcgt gaggcccact cccccgccgc gggcgttgac gaacaggcgc gggctgcgcc
      841 ggcgtcgaag caggatcgac cgcgactgct gcaggtagcg cgagacccag gctgacgcct
      901 cgtcgccgat cgggatgatt cgctgcttgt cgcccttacc ggtgcaggtg agataggagg
      961 cttccaggtg cacgtcggcc ggtctcagcg tgacgagctc ggagacgcgc attccggtcg
     1021 catacatcaa ctcgatgacc gcgcgatccc gcagcccccg gggcgtcttg acgtcgggct
     1081 gggcgatgag ccggtccacg tcctcgaccg acaggtagcg cggcaggact ttccacgccc
     1141 tgggcgcatg aagatcttcc gcggggttgg cgcgcacgcg tccgtcgacg aggagaaaac
     1201 ggtagaaccc gcgatagcac gcgacggcgc gcgcgacgga tcggggcgaa cgtccctcgg
     1261 acatccaccg ccgtacgagc gcctcgaggt cctggcgcga caactcgtgc acgcggcgtg
     1321 ctcgagcgtc ggcgaactcc gcgagatcgt tcagatcgcg cgcgtagctc tcgatcgaat
     1381 tcgcagcgag gcggcgctcg accgtcaggt gggtgaggta ggcatcgagc gccgggacgc
     1441 tcttcggcat cgggatgttg ggctggccgt gccgcctgcg gtgcggcggg cgggcgcggc
     1501 aatccgctag aattgtagcc gtatgcccgt gcgttcgaag tggcagtccc tcccgacgga
     1561 agccatcaac gcggtcagcc tgggaatcga caagctcgct gccgacgaca tcgtcgaatt
     1621 gatgctcaat gaagaccgcc acatgctcga cgcggtgcgc cgcgagcgcg agcggatcgc
     1681 ggtcggcgtc gagatgattg ccgcggcgct gcggaagggc ggccggatcg tgttcgtggg
     1741 agcgggcacg agcggacggc tcgggattct ggaatcggcg gagatgccgc ccaccttcgg
     1801 caccaatccc gacgatatcc aggccatcat ggcgggcggc cgggaggcca tcttcgcggc
     1861 cagggagggc gtcgaggatc actacgagga gggcgcccgc gcgatcgcgc ggctgcggcc
     1921 gacgaagaaa gacatcgtca tcggtgtttc ggcgagcggg atgacgcaat tcgtccgcgg
     1981 ggcgttgacg agcgcccgaa aagcgggcgc ccgcatcatt ttcgtcacct gcgatcccgg
     2041 caccgaactg cagacgttcg tggatctcac aatcgcgccc ggagttggcg ccgaagtgat
     2101 tgcgggctcg acgcggctca aggctggtac ggcaacgaag ctggtcctca acatgctgac
     2161 gacgggagcg atgatccgga ccggcaagac ctacggcaac ctgatggttg acgtccagac
     2221 gggatcggag aagctcaagg atcgcgcccg ccgcatcgtc aacatcgtca ccgggctcga
     2281 gtacgaggac gcggaccgcc tcctgaaggt ggcgcagtgg gacgtcaagg ccgcaatcgt
     2341 gatgcagaag acgggccttc cctatacgaa agcgttgtcg cgccttcgca gggcgcacga
     2401 ctcgatgcgg gaagcgctcg gcgaagacat cgagccccgc cttcgcggac tgctgcaggg
     2461 gcatgcggag gcggcggccg tggccaacga gttcagctcg atggcgtccg ggagcgccca
     2521 gacgcgcagg cgcccgaagt catagcgccg acgcggcggc cggatcgaga tagacctcga
     2581 cgtgccggtg cagctggaga aacgacgccg gcagctgcgt tgacaacggt ccccggatca
     2641 tccgctccac gacggccgcc ttgtgctgcc ccgtcgcgat cagcaggatc gcctcggcgc
     2701 ggaggatggt cgccattccc atcgacaagg cctccgccgg cacacgggtc agatcgcctc
     2761 cgaacaattt ggcgttgtcg cgccgcgtgg actcgagcag cgccacgcgg tgcgtgcgcg
     2821 acacgagcgt tcgccctggt tcgttgaatc cgatgtgtcc gttcgcgccg attccgagaa
     2881 ggagcagccc gaggcccccg gccgccacta tggcatcctc gtagcgacgg cattcggcgt
     2941 cgtcatcggc caggccgcgc agcgagtgaa cccgggcctg gtcgaagttg accaccgaca
     3001 gcagctgctc gccgagctgc cgcctgaagc tgcccgtatc cgagccgtcg aggccgacga
     3061 attcgtcgat caggaacgcc gtcgcacgag agaagtccgc ttctcccgcc gcatacagcc
     3121 ggcgcagttc cgcatacaca tccagcggcg tgcgtccggt cggaagtccc agcacgaggt
     3181 ccggtgtctg tcgaagggcc tcggccacgc ggcgcgccac ctggcgcccc acctccgagg
     3241 tttccagaat cctgatgtcc acgagcgttc agatgtaggt gggcagcctg agcgtgccgc
     3301 gggcctcggc gagcgccagg acgaccgcgc cgcacgcggg ctcgatgtcg agcggccgaa
     3361 cctcgctgcg tggagccact tcggcccatc gcgtggtgac gtcggccgca agcgcgggga
     3421 tggccgagaa gatgccgccc gacagcacgg tcggaaagac gtcgccgcgc atgcccaggc
     3481 gcgtgacgac ggacgctgca gccgccacca gttcgccagc ggcacgcgag aggatctctg
     3541 ccgcaacggc atcccccgcc agcgcggcct gctggacgag atccgccagc cccgcgaccg
     3601 ctggacccgg gagcccgcgg tcgtaaacgg catcgatcag ctccacgggc gttccgagct
     3661 gcatctcctt cagtaccaga tcggtcaatc gggtcgcggg cgcgcggccg tcgaaagcgc
     3721 ggaccacggc cgagagcgcc gcgcggccga tccagaagcc gcctccctca tcgccgagca
     3781 gatatcccca tccgcccgcc ctggcggcgc ggccggcggc gtcgcgcccg tacgcgatcg
     3841 accccgttcc cgcaacgatg acgacgccgg gttgatcgcc ggcccccgcg accagcgcga
     3901 cgagcgcatc gctgaccacg acgacgcgtg tcttgaggcc gatgcggcgc atgatgtcca
     3961 gcaccgcttt cacgtcgccg gggcggtcgg cgcccgcaat ccctacgcag atcgcgtcgg
     4021 gcctgacgtc atgctgagcg agcgcctgct ccatcacccg atggagcacc ttctcggttt
     4081 ccagttcgcc ggcggtctgg aggttggcgc cgccgccgtg ggcctcggcc agtacccggc
     4141 cccgctcgtc ggcgagcaga catactgtct tcgtcccgcc ggcgtcgata ccgaggacat
     4201 gcatgtgaac gagtcaagta tggtacattc gcacattcga tggcgtctgg cgtatcccgc
     4261 ctcgtctgcc tccacgctcg ttcggccatc tggatcggtg tcgtctccgt catcgccgcc
     4321 gcgcacgccg tggcgtcggc cgctgacaac agtcccgcga gagcgtttcg gattccgtcc
     4381 gccgcgatga cgtcgccagc cgcgatgcgt cgcgcgatct cggcggcggt ggccggcagt
     4441 ttcgatacgg tcgttgtccc ggtctctgcc actccgcttt ccgacgcgaa tctcgccgac
     4501 agcgaagcgg aactggtgcg tcaggctcgc gagaacggcc tgcgcgtgca cctggcgatt
     4561 gccgtcaacc ttgcggtcgc gacgggcgag ttgccctccg cgcgcgagca cgtgatctac
     4621 cagcaccccg aatggctgat ggtgccgcgc gacgcggccg tggagctgct cacggtcgat
     4681 gcgcgcagtc cggcgtatct ggggcggctg ttgcgatgga cccgcgccaa cgcggatcgt
     4741 gtcagcggca tctacgtctc gcccctcgat ccggatgcgg cgtcgtacct ggtcaacgcg
     4801 gtcacgtcgg cactggctcg tcacgccgcc gacggcgtgt acctcgaggc tctcgacttt
     4861 ccaggcgagg acttcgatta cagccggcgc gccctggatc tcttccgggc ccggacacgg
     4921 aacgccctgt cgccggccga acgggcgcgg gtggacgaga tcgaggcgat cgatccgttc
     4981 gcgtacgagg aagagtttcc cgacgcgtgg cggacgttca gggaatcggc gctcacgagg
     5041 ctcctcgaac gtctccgctc caccctggtt gcggcgaatc cgtcgctcgg cgtgacggtc
     5101 agcgcctcgg ccgatcccga tgtctcgctc cgccaccact tccagaactg gcgcgcgtgg
     5161 ctcgcaggca gaatcgtcga tcgaattgga taccccggac cttcgagcgg tgtcgttctg
     5221 ttctcggccg agggcgccgc tcccgccgat tcatccccgc tgacgagcgc gcagaccgcc
     5281 gtcggcgatg gttcgcggta gaggcgcaat ggcgcctcca ttcgccgccg cgcgcgcggt
     5341 gatcgagcgc gcggtcgcgg accgcgcctt tccggcggcc gtcgtcgagg tcggcacggc
     5401 gcatcggccc ctctggtgcg aggcgttcgg ccggctgtcc ttcgataccg gtgcgaggcc
     5461 agcccagaca gacacggttt tcgacctcgc gtcgctgacc aaggtcctcg ccacggttcc
     5521 gctcgtcatg cgcgccgtcg agcgcggcaa ccttggcctc gacgacctgg tgtccagcca
     5581 cgtcccctcg tggcgcgacg atggaacggc ctctccggtg acgcttcggg acctcctggc
     5641 tcattgcagc ggccttcccg cttacgcgcc cttgttcaag gaacactccg gccgtgcggg
     5701 gttcgaatcg gccatctgca cgatgccgcg cgagtacgcg ccccgttcaa ctgccgtgta
     5761 cagcgatctc gggttcatcc tgctcgggtt catcctcgaa gacatcgctc ccctgccagt
     5821 ccagttcgac acgatcgcgc gacaaatggg aggcatgccg gacatccagt tccatccgcc
     5881 agagatctgg cgcgtccgaa cggcgcctgc gcgcgacgac gattggcgag gacgcgtgct
     5941 tctaggcgag gttgacgaca acaacgcctg ggcgctcggc ggcgcggccg gccatgcggg
     6001 cctgttcgga accgcgggcg ccgtgggcga gtgcacgcga cacgtgcttc aagtgctcga
     6061 gggaaggacc ggcgcgttcc ggaaggacac gctgctgacg ttcgtcaccc gccgcacgga
     6121 cgtgcccggc agctcgaggg cgctcgggtg ggacacgatg ctgccaacct cgtcgtgcgg
     6181 ggcgcgcctg tcgccgcgcg ccttcggcca cacgggcttc acggggacgt cgttgtggat
     6241 cgatccggag cgcagcgtct acgtcgtcct gctgaccaac cgcgtgcatc cgacgaccgc
     6301 aaacgaagcg atcaaggccg tgcgccggtc gctgcacgac gcggtcattg aagatctgga
     6361 ccggtagtca ctcccaaatt cccagttccc aacgcccaag aaacgtacaa atcgcgagcg
     6421 cgtgtgattg ggagttggaa gttgggagtt ggaggttggg acttggttca gagtcccccg
     6481 cgcccccggc cgccgcgtgc gggcgctggt gtcgcgcccc tgcgtccgcc gggcgccgcg
     6541 ccgccgcgta cgccgggtgt ctgccccccg acgcctggag gcgcgccggc ggcggtgctc
     6601 ctctgctggg ccatgaagac ccattcgttg tacttgtcct tgccgttgta gagccgtatc
     6661 gaggtctgcg tgctcttgct ggcgacagcc agcactcctc cgggggcctg accgctcgcc
     6721 gctccgggct gagtgaaccg cgacacggcg aagggctgag tgggctgctg tgtctgcgtc
     6781 gagccccgag cccctccgcg cgcgggctga gcagcgcctc tgcccgtcgt ctgcgccccg
     6841 cggccggcgc gtcccggcac ctgtggcggc gtgttcagcg cctgcgccaa ttcggggctg
     6901 ccggggccga ggatcagaaa gtctcccccg gtgatcgggt ccttgtactt cttgcgcagg
     6961 aagcggtccc tgatcagggt gtcaacgtcg ggaggcaacg cgttgccata cctgccctga
     7021 aacattccga tggcgcgcgc gtactgctcg ccgcgaaaga cgagctccgc ttctttctca
     7081 cgtcgagcaa cggtttgaaa gaccggaagc gcagtgctga tcgcgatcgc catgacgctg
     7141 atggcgacga gcagcgcggc cattgcgtag ccgcgctcgc tggtgatcgc gcggccgtgc
     7201 ttcatcagaa gctccccaac agaatttgag tcgtcgcgac gagggcctga ccgttcgtga
     7261 gcctgatcgt cactgtgacg acgcgcgtgg tattcgcccc agacgtgtag acatgggacg
     7321 tgacgttgct gttcgtcgtc gcggtcgagc cgtccccgaa atcccagtcg tacgccgcgg
     7381 cctgggcgct cggatcgttg ggcggcgtga gggtcgccgt aaagttccag gtctgtccga
     7441 tcccgatctg aatcgcaccc ggagccgcgg tcagtccagg caccaccgga tcgcgtcgcg
     7501 tgacagttgt ctcggccgtc ttcaatccgg cggaagcggt caccttcgcc gtttgcgttg
     7561 tcgtcagcgt cgtccgggcc tggccgaacg cgtcggtcgc gacgcgcgtg ctggcaagct
     7621 gcccctcggt ggatgcgaac aacacgaaca cgttcgacat ctcgcgcccg ttcgtgccca
     7681 gcacagtggc cagcagctcg acggtccccc cggccgctgg cagcgacccg ggattggcgc
     7741 ccaggagaac ggtttcgacc gacgcggagc ctacgctgat ttggacgacg ttggtcgccg
     7801 agccgttcgc gccctcccct gttgcaccgc cggcggcgcc cgaccaggca ctgacgtcag
     7861 cgatgcccga tacgtcgccc gcggagaacg tggcgacggc gtatccgttc cgggtctgcg
     7921 cttcaaccgg gtcgacgcgc cccaggttgg tcgagaagcg aacagtcgtg ccgttctgga
     7981 cgggtgtgcc gctggactcg tacacgaacg ccgtcacctg ggtcgttcca ccagcaggta
     8041 caacgacgct cgccgccgtc agcgtgacgg tcgaaccggt cggcgcgagc aactgcaccg
     8101 tgtcacaggc tgcggctgcg ccggcgagag cgatccagag gccgacgtaa acgcgacttg
     8161 gcatcctcat tcgaactccg cgtacggcgt gttgtcgatt gacacctgat ccgacccgct
     8221 cttgacgttc tcgatgccgg gctccgcgtt cgggtttccg gggtcgaact ccgccggaat
     8281 ctcctgccac gtctcgacat tcccggtgat gtccttcggg atactcctca ggtacttatc
     8341 ggtgacgagc gcatcgagac tcgcggggta cttccccttg tctgcgtagt attcgtcgat
     8401 cgccttgcgc atctgccaga gattttcggt aagcgcggcc tctttcgccc gctggacgtt
     8461 gttgccgtac acaaccagcg acacgctagc caggatcagg acgagggtca ttacgacgaa
     8521 cagctcgatg agggtccatc cgccgcaccg ccccgccttc acacgccgtc gctctcgtag
     8581 catcgtctta ccaatcccgg tatttcgttc cgtcgagcgc cgtccccgca ctcgtcgtgt
     8641 acacgtcgaa cacattctct ccgccccacg acgaggaatc cggttcgtcc tgatacgacc
     8701 gcaagcccca gtcggtgctc ttggtcatcg ggtcctgcgg gatccgccgc aggaacttca
     8761 gcttcctgcc ggttgcgtcg ttggccaccg tgacgccgtc gacgagggtc tcgaggtcgg
     8821 gcgggtaccc ctcgctcccg gcctctacct ccgacctcgc aatctgtccg cgatcgtagg
     8881 cgtccttgaa ccggtcgatc gccgtccgca tctcgcgcag gtcccggcgg agctcgacct
     8941 cacgctggcg ttgtgcggtg acccgggaca ggggcagcac cgttgacgac aggatcagca
     9001 ggatcgttgt cacgatgacc agctcgagga acgtgaagcc gcgctggttg gtcacttacg
     9061 gtaccctcac ggccggcact ggcacgaact gcagattgac ggcactaccg ccgggacccg
     9121 ttgccgagcc tgtgacaccg agattcgcgg caccggacgc gacggcttcg aacaggaggc
     9181 cggcgagcag accggtccct gcaacgccag tggggtcggc tcgacgaacg atcgcgatgt
     9241 ctactcgccc accagccgga tccgcctgct ggctgaacga cgcgctgacg ccgccagcgc
     9301 gcatgaagct cccttcctgg acgttcacga gacgaagaac ggcagggttg tacgacacgg
     9361 tcaacgtgat ccccgagagc tgtgaagcgc ccgtgatcga caaggggacg aggtacgggc
     9421 cgccacccaa tcggaattca ggtccgggta ccgacatcag cacctgaccg gccgacgccg
     9481 tcgccgggcc gggttttgac gcacccaatg gctgggcgcc cgcggtcgtc ccgggggccc
     9541 cggccggggg cgccggcgtc tgaggggcgg gcgcctgggc cgttacggcc ggcgtctgcg
     9601 gtgcgggacc agccacggtg cccggggtcg catccggcac gggcgggcca ccggccacgg
     9661 gcgccaccag cggcggcggt ccgctgaggc cgaagttgga ctgcgtgccg atgtacaccg
     9721 gagccaggtc ctgcggcgtc agctcacgcg tgcggacgat gcgcggcgtc agcagcatga
     9781 cgatgtcgct ttgctggatc tgttcgtcgt tgctcgagaa cagctgcttg atgattggca
     9841 gattcagcag ccacggcagg ccggagagca ccttgcgctc gctctcctga agaaggcccg
     9901 ccagcagtgt cgattcgccc tctcgcagcc tgatgtcggt ctccaccttc cgcgagttga
     9961 acgagggaag ctcctggccc gcgatcttcg tgccgggacc gatgctgctc gtctcgagct
    10021 tcagcttcat ctgaatgtcg ccctcgaggc tcacgcgagg gttcatctcg acgatcacgc
    10081 cgaccgatcg gtattcgaac gaggtcaggg gattgaagtt cgccccgccc tgcgccagcg
    10141 gcgtgaacgt cgtcgagggt acgggcacgt cttcaccgag gttcatcctg atatcggagc
    10201 cttcggcgcc ccgcagctgc ggcttggcga tgaccttcgt ttcggagtcc gtctccagga
    10261 agcggatgac cgccgagggc acggccaggt agaagtcgtt ggcgctgatc cctcgagtga
    10321 tggtgttggc gttgaaggcg cgcgacagcg tcgcgttgtc ctggttctgg tctccatttg
    10381 tcggcggggt gacgacagcg cggatgtcag cctccggaga gaacacggcg ttgatcgagt
    10441 acgacccgag atccaacccg tactgacgga cgcggccctt gttgacctcg agaatctgca
    10501 cgtcgatgac gatctccgcc ctcggcttgt cgaggatgtc gatcaggcgc tcgacgatcc
    10561 ccatcatcgc cgtggtgccg cgaacggtga tcgaattgtt cgtcttgttc ggcgcaaact
    10621 gcggcgccag ctgctgcccg gccgcccgaa gcactgtctg gagcgactgc atgatctcgg
    10681 acggatcgcc gtacgagagc gggaaggtga ccacgacctg gtcctcgtac tgcgtgcgct
    10741 tcggcgcggt gtcggggatg atcatgatcg tcttcgggtt catcaccttg tagaagagct
    10801 gattcgcctg ggtgatctgc tgcagcgcct gctccagcgt cacgtccgac atggccacgg
    10861 tgtaaacgcg gtcctggtag gtgctctcgt aggtgacgtt gatccccgtg ccttcgccga
    10921 tcgagttgag gatgtcgcgc aggctcgcct gctggaactt catcggctga acgatggtgc
    10981 tgaagttgaa gagcggcggc gccgccccgg cgcgtgcggc ctcgcgcatc tgctggggcg
    11041 agggccgctt ggccgcttcg atctggtcgc gtatggtgcg ctcgagatcg agcaccttct
    11101 gggcgactgc cctgttcggc gggtcgtact cgctcgcccg gcggtactcg cgcaacgctt
    11161 catcgagctg tcccctcgcc tccgccaggc gggctttgtc gaggtggccg atcgaggcgc
    11221 tgatcatcga gcgctcgagc gcgatcttgt aggcggggtt ttgggggtcc gcctgcacgg
    11281 cctcgcggta gtgcacgacg gcggcgtccc agtccccgcc ccgtgccgct tcgtcaccgc
    11341 ggcggaaggc gttcccggcg gcgcaaccgc ccgcaaacag ggcaacgacc acgaggagcg
    11401 ctgcggcgtg tgagtgtctc attgtcctga caaccgaatc gtttgccggc cgcggccgtc
    11461 cacatacatg agatcggcgg aatcgacccc gacccgtagt acgcggtagc ggccctcgat
    11521 gatatcgccc tcgcgtccgt ggaagacgtt gccccgcccg tcgctgagca cggccacgcg
    11581 tggaggccgg ttcgcctgat cgacgtagcc gatgaaccga agtggaatcg gcggcggcgg
    11641 gggcggctgg ggcggaccgg ttggaaccgg cggggcgagc gtgacctgct ggcgggacgc
    11701 cgcgggagcc ggcctggcgt ggaatcgaaa gaggtctcga ccgagcttga cgcggtcgtc
    11761 gccacgctcc tccagcagtt cgagcctgag gtctccaacg gccgactccg ccccggccgc
    11821 cggcgctccc gttgtgcctg gcgcggtgga tccggcgccg gtcacgtcag aggtcggcca
    11881 ttgtctgtac acgagcagtc cgcccgccag cacgagggcg ccgaggacca gcagttgaag
    11941 gcgtgttttg gtcatcacag cccgccggtc cagaaatagg ttgacagctc cagcttcagc
    12001 cggagcgagc cgtcacccgc ggtcccttcc gacagggaca cgttgtcgat gacgacgaac
    12061 tcgggcgccg tttcgatctc gtgaatgaag ttgcgaatgt cggagtaatc gccagtcagg
    12121 tccatcgtga ccgcgaactt ggtgaggttg ctgccgcgtt cgctcaccgg ttcgtaggtc
    12181 ccgtgctcgt agtccaggct cgcgtcctcc gcgagccgcg ccaggcgcgc gtacgtcagg
    12241 cggcgggccc cggccaggtc gggcgccagc acgctcgtat agaacgtatt gagttccttc
    12301 tgtgctcgat ccttgcctgt cagcaggccg ttggcggtgg tgaactcgcc ctttgccgcc
    12361 gcctcggcct gctcggcgct ctgggtccgc tgctcgacgt tggccacggc ggcgcgcagc
    12421 gggtacacga gaaacgcaaa cacgagcacg ttgacgacga cgccgccggc gagggcgtac
    12481 accgtccggc ggcgctcggc caggatgcgg cgaaggagcg tcatggcgtc actcaccggc
    12541 ggctcctgtc gtgacgggcg ccggcggcgc cggccgcgga ggctggatgt acacgccctc
    12601 gatcagccca tcgatcaacc cctccaggtt ggtccgctct tccgcggcga gcacggcgtc
    12661 gaacctgtcg gatttctcga gcgcgtcgag aaacgtctcg aggtccctca ctcgacgggc
    12721 caggacaggg acactcacga cgaagcggcc ttccgtgtcc acgcgcgact tgaaaccggt
    12781 gagacgaacg tcgtcgggca gcgccgcctc gagctgggcg aacaggtcgc tccaggaaaa
    12841 cgtccgcagg tcgatgatgg cgttcgcctc ctgggccgcc tccgcgactt cggtgagctc
    12901 cctcgtgttg atttgtccgc gaatgcgcct cgcatcgacc cgaaggcggg cggcctcctc
    12961 ctcggcccgc gccgcgcgcg cgcccagatc gcgttcgctc gcgctcagcc tgacgagttc
    13021 gacgatgttt cccagcgtca tcacggcgac gatcaccgcg agagcgccaa tcgtcacctg
    13081 gaccgcgcgc aggttgtaga acggccgtgt cgagaggttc gtgcgcagca tcaggccgcc
    13141 accccctccc gctgcgatcg gagcagcatc cccacgagcg gcgcgagcgt ggccatcaac
    13201 tccggcgtcg cgtggatccg gtcggtcagc gcggccccgc aggtcggatc gatcggttcg
    13261 acgatcgtac ccatacgttc ttcgagattg cgccgggcga ggtcaacggc gcccggtgcg
    13321 cgcccgatgc cgccgagcag gatccggctg aagccgcgcc cttccaggcg atcctggtag
    13381 tacatcgccg tctgatgcac gacgtcctcg agcgcgtcgg gatcgccctc agcccggctg
    13441 cggaagaaca tcatgtcctc gcggcgaagg atcgccatcg aggtgtactc cggccgcatg
    13501 tggacgacca gccagtcgcc cgaggtgcca aacccggcgc tcaagaacag gttgatgacg
    13561 ctgaacgtgg aggtatcgac gaggccggct tcgatgcccg cgtcttcgca cgccccctcg
    13621 tattccctga tgatgtcgcg cctggccacc accaccacga actcgtgcgc gcctgcggcc
    13681 tgtccgccca gcgtgtaagt caggcacgcg tcctcgaccg ggaagggcgt ggacttcttc
    13741 acctgccacc ggagcaattg cgtgaggtcc tcccggcgcg ccggcatctg ctcgaatcgt
    13801 acgagcgaca ctttcgtcgt cacatcgggg atgaccagcg ccacgcgtga gggcttgacg
    13861 ccgatgctct gacaggccgc gtggagcgct cgcactacgg ccgtgcgatc gacgatgttc
    13921 ggcgcggtga gcgacggaga gacggcgccg gccgggagcg gctcgacggc gtagccctgc
    13981 acgacggcgt tcacgccgac gcggccgttc agtacggcga tcgagacggc ggacgccgag
    14041 atctcaatgg cggcctcgag cggcggcgtc gcgagcagtg aagtgagcag gctcattcga
    14101 cgaacgtcac cttgttgatc tcacggagcg tcgtcaggcc ggtgaacacc ttctcgaccg
    14161 ccgattctct caggaagcgc atgccttcct cgtgagcggc cttcttgatg tccgacgccg
    14221 ggcgcctctc gagaatcatt tctcgaatgc gatccgacag gtcgaggagc tcgcagatcg
    14281 ccgtgcgccc cttgaagccg gtcccgccgc attccacgca tccggcgcct tcgtagaacc
    14341 ggcctgtctg cgccagctcc tccggcaggc cggcctcatt gagcgccttg gcgtccacac
    14401 gagccggccg ccggcagtgc gtgcagatca gccgcaccag ccgctgcgcc atcacgcagt
    14461 tcagggccga aacgaactgg tacggctcca cgcccatgtt cagaaaccgc ccgagcacgt
    14521 ccacgacgtt gttggcgtgc acggtggtga acacgaggtg cccggtcagc gccgactgga
    14581 tggcaatctg ggcggtctcc ggatctcgaa tctcgccgac catgatcttg tcggggtcgt
    14641 gccgcaggat ggaccgcagt ccgcgcgcga aggtgagccc cttcttctcg ttgatcggga
    14701 tctgggtgat gccccgcagc tgatactcga ccgggtcctc gatggtgacg atcttgtctt
    14761 cgacggactt gatctccgac aacgccgcat agagcgtcgt cgtcttgccg gagccggtcg
    14821 gcccggtcac caggaccatg ccgtatggct cgcggatgta cttccggaag cgccgcagct
    14881 cctgctccgg gaaaccgagg atgtcgagct tcagctcggt gaacttctcg ctgatcgatt
    14941 ccttgtcgag gatgcggatc acggcgtcct cgccgtggac gctcggcatg atcgacacgc
    15001 ggaagtcgat cgtcttgccg ggcatccgga ccttgaaacg cccgtcctgc ggcacgcgct
    15061 tctcggcgat gtccagttcg gccatcacct tcacgcgcga gatgatcgag ctgtggaact
    15121 gtttgccaat cggacgcatg gccggctgga ggacgccgtc gatgcggtac ttcacgtgca
    15181 ccgcgtcgtc accggtctcg atgtggatgt cgctcgcccg gcgctggatg gcagtgtaga
    15241 tcgtcgagtc caccagcttg atgatcgggc tgatgtcgct cgtcagccgc tcgaccgtca
    15301 ggctctcctc gccggtgtcg tcttccttca gcacctggag ctcgaagccc tcgctcgcct
    15361 cttcgaggac gcgctgcgag ctctcgctct tcttgaggat ggactggatg gccgacggca
    15421 cgccgacggt gacgcggatg ggcgtccgca ggatgacgcc aagctcgtcg atcatcggga
    15481 ggtcggtcgg atccgagacg acgatgacga gcgccttgcc ctcgcgccgg tgcggaacga
    15541 atccgtaccg gagcatcaga tcggccggaa tcgaccggaa gagatcctgg tcgatccgga
    15601 actccttcat gtccacgaat tccagccggt agcgcgcggc cagcgcctgc gcgcgcgtga
    15661 cctcctcggg aaggtccggg tccggaacgg gcagcgggtt cgttttgacc gtcgccatca
    15721 gagagacacc agcgagctca gctcgaagag cggcatgtag agagcgagca cgacgccggc
    15781 gatgatgacg cccatgccga ccagcagcgc gggctcgatc atggtgatga atcgcccgac
    15841 ttcggtatcg acttcctcgt cgtagaagtc ggcgaggctg ttgagcatct cctgcagggc
    15901 gccggttgac tcacccactt ctatcatctt tacggcgacg tcggggaatg ccgcccgctc
    15961 gcgcagggcg gcggcgaacc cctgcccttc ctgcacgcga acccgtaccg tgtcgagctc
    16021 gcgcgccaga tggcgattcg agatcgcccg cacgctgatc tcgagcgcgt tgaccagcgg
    16081 gataccaccg ccgaggagcg tcgacagcgt gcgcccgagc tgcgacatgt agaacttgag
    16141 cgcggtcgtg ccgacccacg gcacgtcgag cagccagcgg tgcatgcgct cgcgctgctc
    16201 cggcagccgc agccacgaca tcgacaggac gaccagcgcc acgaccccgc cgaggatgag
    16261 gccgatgtga ttggtgatcg tgttggagaa ggtgacgatg acgcgggtca acagcggcag
    16321 cggcgcctcg aagtttgcgt agaattccga gaaggccggc accaccttca ggacgatgat
    16381 cgagacgagg acgatcatca tcgcgatcag aatcgccggg tagatcatcg ccgaaatcgt
    16441 ccgccgccgt accgcggtga tgatcttctc gtagctgacg taacggcgaa tcacggtgtc
    16501 gagattgccg ctccgctcgc ccgccaccag cgacgccgaa tagaccggcg gaaagagcga
    16561 ctggtgctcg gcgaaggcgt ccgagagcgc cacacccccc ttgaccttgt cgtgaacgct
    16621 gtccaggacg gtcttgaacg tccggttcgg gacgcgctgc cgcaggatgt ccagggactg
    16681 gaccagaggc atcccggcct tgagcagggt cgccagttcc tggttgaaca cgaggaactc
    16741 gtggctcttg atccgctgcc gcccccggcc gaaggacagc gacagccccg cccgacgaag
    16801 ggaaaggacg tacagcccct tttcctccaa ttcacggcgc agaagcgcct cgctgtcggc
    16861 gacgtaaagg ccttctacga tctcaccggc aggcgtgccg agtcgacagc ggaactccat
    16921 cgtgtcagtt tagacccgca ccctactatt tcggctcaac ataggcacgg aatacccgca
    16981 cccccagttg ccgggtgtca ccgctcttca gggcctccag cttggccggg acgaatgtct
    17041 tgtcacttac gactgcaagg tcgacggttt ggccgtcgcc caactggctc tgggtcagtg
    17101 aaatcctgcg caactcggcg ggaccggttg ccagccggaa cgtgtctacc accgagtcgc
    17161 cgagcttgac ctggatggtt tgtggctctt ccacgaacgc gggcgccggc tggtcggcct
    17221 ggatgaccac gacgacgtcc cgcttcgggt tcgggaacga gagcctcccc gacttcatcg
    17281 accatcgcca ttggactccg gacgtcttga ccgattcggg agaatgccac ccctcccgaa
    17341 acgacaccgg gtagggatcg tccagatcgg tcaaagtcaa cgatccaacc gtgaccgccc
    17401 gtggctcgaa gagctctccg gcgattggca cccgttcgtt gctcgaaggc aggtagaggc
    17461 ccacgaccac cttcatgtcg ccacggtagt cggccgtcgg aggaaacgcc gtgtgtgaat
    17521 actccaccgt cgaccccgcc ttccattcac gcgtcggggt tggcggtggg tgatccgcgg
    17581 caccaatcat cttgcccttg tcgtcgatga agtgcacgaa gacgacgtaa tccgcggtga
    17641 acgcgggcgc gtccggcgac accgcgaagc gcagcgcgaa ctcgaacggc ctccctgcgg
    17701 tcaccttcgg ggaactcaag ctgaacgaag gcgtaaccac gggcttggcg gcgtcgagct
    17761 cagggcccga acagcccgcg atcagtccgg cgacgagcac cgtacacacg gagtatctcg
    17821 gcatcgcggg aatgatacta tctcccccgc atgtcatccg atgcgctcgg catggtggag
    17881 acccgcggcc tgatcggatc tgtcgaggcg gccgatgcca tggtgaagac cgccaacgtc
    17941 gtgctggtcg gcaaggagta cataggcgcc ggctacgtca cggtgctggt ccggggagac
    18001 gtgggggccg tcaaggcggc caccgacgcg ggcgcggccg cggcccgacg cgtgggcgag
    18061 ctcgtctcgg tacacgtgat cccgcgacca cacaccgagg tcgagcgcat tcttcccaag
    18121 ccatagccgc gatcgcggag tgtgcagatg gctgtccagg acgcagcacc ccataccgac
    18181 aaagatcttg cgtccatagc cgagtcgcgg gcgctggccc gcgcggccag gcaagcgcag
    18241 ccgttactcg cagagttcag ccaggaacag atcgactcga tcgtcaccgc catggccgac
    18301 gcggccacgc gggaagcgga agcgctcgca cagctcgccg ttgacgagac gggctacggc
    18361 gtcgtggcgg acaaggttca gaagaacctc ttttcgtccc gccaggtcta cgagttcatc
    18421 aagccgctca agacggtcgg cgtcgtcaat cggatcgacg atcggaagat cgtcgagatc
    18481 gccgagcctt tcggcgtcgt cgcggccatc gtgccgtcta ccaatccaac ctcgacggcg
    18541 atctacaaag tgctgattgc actcaaggcg cgctgcccta tcgtgatcag tccgcacccg
    18601 tccgctgcca ggtgcatcac gaggactgtc gaaatcatgt cggaagccgc gtcgcgcgcc
    18661 ggtgcgccgg ccggcagcat cggctggatg aagaccgtta ctctcgaggg cacgcaggag
    18721 ctcatgaagc accgcgacgt ggccgtgatt ctggcgaccg gcggcatggg cctcgtccgc
    18781 gccgcgtaca gcgccggaaa gcccgcttac ggcgtcggac cgggcaacgc tccctgctac
    18841 atagaacggt cagccgatct cggcaaggcg gcacgcgaca tcgtgctggg caagacgttc
    18901 gacaacggcg tgctgtgctc gtcgcccaac tcggtcgtgg tcgatgagtc cgtcgccgcc
    18961 gaagcccggc ggcagttcga ggcgcaaggc gcgtacttca tgaacgacgc agagagtgat
    19021 gcgctcgcca agcagctcgt cacgccccag cggctgccca accctgccct ggtgggaaag
    19081 tccgcagctc tgattgcgga aaaggtcggc ctcaaagtcc ccgcgggcac gaccgtgctc
    19141 atcgccccct tgatgggcgt cggacgcgat tacccgcttt cgatagagaa gctgtgcccc
    19201 atcctttcct ggtacgtggt cgcggactgg cgcgcaggct gcgagcggtg catccagatc
    19261 ctgcgctacg gcgggatggg gcatacgatg tcgatacact cgaagaacga ggaggtcatc
    19321 ctcgagttcg ggctgaagaa gccggcgttt cggatctgcg tcaatacccc cactacgcac
    19381 ggctccatcg ggctgacgac ggggcttgac cccgcaatga cgctcggctg cggcggctgg
    19441 ggaggaaaca tcacctcgga caacatctcc ccgcgtcatc tgctcaatat caagcggctc
    19501 gcctacgaac tgcgtccagc gacccgcgca acggctgacg ttggctcagc ggcggcgaag
    19561 ggcggccagt ccttaccggc cgcgcccgcc cggcggccga tcgagggcat ccccgccgag
    19621 gcgctttcga gcaggatcga cgcgttcctc tcgtcgcgcg gcttccggcc agcccagcaa
    19681 cgggaggccg ccgccaccgg cgccaagaaa aacgagccag tagccgatac tccggaagcg
    19741 ttcatctgtg aggacgacgt gcgggccgcg atgcagtcgg ggcgaaagct gctggtcggg
    19801 gaacggacga tcgtgacgcc tgccgcgaga gatctcggcg agtcggcgag agtcttcgtg
    19861 aacgcaggct ggccccattg atcagtgatg caaacttctg cgcgcgaatg cccgtccaaa
    19921 ggggtcgtgg ctttgaccct ccgatcgagc ggatgctaga gtaccgaatg ctcaccccga
    19981 gccgcgcgtt gaccgtggtt tgcgtgttgg gagtgctggc gaccggctgc ggaaagcgct
    20041 tcgcacctgc cggcgtgagc gagattccat ccgcggaggc ggtaccaagc ccagccgcag
    20101 tcacaccgcc gctcgacgcc gagccggccc cgtcccctga gcctgtcacc ccggcgcagc
    20161 aggctcaggc cgagccctcc gaaaccaagg aagcggaagc gcaggcagat ccgaatttca
    20221 aggagaaggc gcctgagccg gcgccgatcg acgaactgct cgctatctcg accgtcgaga
    20281 cgccgcctcc cacgccgaag accgagaagt cggtcgccga cgacctgcag accatcgttc
    20341 acgacatcga cattccgcag aacgcccgcg tcctgcgcta tgtcgagctg ttctccggga
    20401 agctgaaggg ctatctcgaa gaaggccttg gccgaggcgc ccagtatctc ccgatgattc
    20461 aggacgtgtt cagggccgag ggtctcccgc tggatctggc ttatgtgccg ctgattgaga
    20521 gcgcgttcaa gccgaccgcg ctgtcccgcg cgaaggccaa gggaatctgg cagttcatgc
    20581 gcggcaccgc gctcgagaac gggctcactc acgattggta catcgacgag cgcgccgatc
    20641 cggagaaagc gacgcgtgcg gcagcgaggt acctcaagac gctgcacggc atgttcgacg
    20701 actggcacct ggcgctcgcg tcctacaacg gcgggcccgg ccgcatccag cgcgccatga
    20761 agcgatcggg acgcgacgac ttctggaagc tgtcggcgaa cagccgctat ttaccgcgtg
    20821 aaacgcgaga ctacgtgccg ctgattctgg ccgcgatcgt cgttgcccgg aatccgggtc
    20881 actacggttt gaacatcgcg cccgtcgacg cgcggccctt cgacaccgtg tcggtcgcgg
    20941 ccgctgtaga cctcaggcgt gtggctgaat gggccgaggt gacggtggcc gagctccagg
    21001 atctcaatcc cgagctgcgc cgctggacga cgccggtgcg cgcgacggtg tacgagttga
    21061 aggtcccgca aggaacggcg gacgcaatca atggcagact ggcgctgctt gccgacgaag
    21121 gcgctccctt cacgcgttac tcggtgaaga agggggagac catccagggg attgcgaaga
    21181 agctcaaggt gacgcgctcg gatctggccg aggcgaacta cctgtccacg aaggccaggc
    21241 tgtcaaccgg ccagcagctc atcgtcccac gcgcgccggc tctgaccgct cgggcctcga
    21301 gcccggccga tcggccccag cccgcagcca cgccagacgt cgtcctggcc agccgcagca
    21361 tcggcgtcga gaaggcagag cgtcccgcgc cagccagcac gatgcatcgc gtcaagcgcg
    21421 gcgagacgct gttctcgatt gcgaagcggt acgggacgac cgtgacgctc ctcaaggaat
    21481 ggaacggcct ccgcggcaac gtcattcaga tcggccagcg gctgatcgtc ggcgcctact
    21541 ccacgaactg aacatccctg cttcgacgaa tctgcgtacc tcgccggcgt gaggcgctgg
    21601 aatttctgcg agtttccccg ccccgcccgg tgccgcggcc acaattctcg aatgattccg
    21661 cgctgagccg gttcttgcac tgatcggcgg gcatcgtgct cgcgtgcctg cggagtgccg
    21721 ccgtcttcgg cgtcgaggcc taccccgtcc acatcgaggt ggacgtcgcc ttcggacttc
    21781 cccacttctc gatggtggga ctgcccgatg cgaccgttcg cgagagccgc gatcgcgtgc
    21841 ggtcggccat ccggaactcg gggttcgatt ttcctcctca tcgcatcacg gtcaacctgg
    21901 cgccggccga cgtgcgcaag gcgggttcgt ctttcgatct tccgatcgcc ctcggcctgc
    21961 tggccacctc cgggcctctt cagcggcgcg ccgttgacga cacggtcatc gtcggcgagt
    22021 tgtcgctcga cggcgcgatc aacggcatca ggggcgtgct tccgatcgcg gtgggcgcgc
    22081 gccggctcgg cgtgaagcgg ctcctgcttc ccccgcagaa cgcggcggaa gcgtcggtcg
    22141 tcgaggggct cgacgtctgc atcgcgaggt cgctgccaga ggcggttgac gcgctcaacc
    22201 ggccggacga ggcgcagcgg gccacgtcag aaccggccgc ggccgtactg ccacacgtca
    22261 acgacggcga cctgtcggag gtgcgggggc aactcctggc gaggcgcgcg ctcgaaatcg
    22321 cggcggccgg ggggcacaac ttgttgttga cgggcccacc aggcgccggc aagacgatga
    22381 tggccaggcg gctcgggacg attcttccgc cgctcacctt cgacgaagcc ctcgagtgca
    22441 cggcgatcca ttcggtcgcg ggcacgctgc cctcggggac tggcctgctc gtcgatcgtc
    22501 ctttcagagc gccgcatcac accatctcgc acgtcgcgtt ggtaggcggc ggtgccatcc
    22561 cgcggccggg agagatctcc cttgctcacc acggcgtcct cttcctggac gagatgccgg
    22621 aattcgatcg gcgcgtcctc gaagtgctgc ggcagccgct cgaagagggg cgcgtgtcca
    22681 tcgcgcgcgc ggcccgcacc gccgtctttc ccgcgcggtt cgtgatgatc ggcgccatga
    22741 atccgtgtcc gtgcggattt gccggggacg agcggcgccc gtgccgctgt acgcccgttc
    22801 aggtcgcgaa ataccgcaac cggttgtccg gcccgctgcg ggaccggatc gatctccatg
    22861 tggaagttcc ggcggtcccg gtgggcgtta tcacggacac ggcggctggc gagccctcgg
    22921 catcagttcg tgaacgggtg ctctcggcgc gccgcctgca gcacacgcgc tacggaccgg
    22981 acgggccgcg gaccaacgcg gcactcgccg gctcccgcat taccgcaatc tgccgccccg
    23041 acgcgaaggg acggcagttg cttcggcgcg ccgtggatca actgggcttg agcgcccgcg
    23101 ggcacgatcg cgtcctgaaa gtggcgcgca cggtcgccga tctctccggc gccgacgagg
    23161 tcggatgtga ccacgtcgcc gaggcattgc aataccgatt ggtggaacac gggctgggtg
    23221 cagaggctta ggtgaagtat gaacgtcgta gtcggtcgtc gggaattcct cggttcagct
    23281 gccatcgtcg gcgtcggttc gatgaccgcc gggccgctgc tcgtggaggg cgcaggtaag
    23341 cactctcacc acacgggagg tcacatcgaa caggagctcg tggatcagtt gcgcaccagc
    23401 ctccgcggca tgcagggcgc aaggccggcc gagggcgcgc gtcagttggc gtccacactg
    23461 cgccttgctg ctgcgcactt caaggagaag gagatagatg cagacctgaa ggcgtttctc
    23521 cattcaacga tccggaacga agggcgtgag agccttctgc ggcggcagat cgacaagcgt
    23581 atgcttgcgg ccgagatgca gaagctcggt gtgacagcgc cgcttgatct cggaccggtc
    23641 gaccacgcga tgcgcgagaa ggcgctggac atgctcctga caacgggact tactccgctg
    23701 atgacccgtg tggccgacac gctcgatcga cgaagcgaga cgctcgaccg tcgggagacg
    23761 atcagactga gcagcgctca gga
//
LOCUS       DBNGSH010000123         4495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4593525, whole genome shotgun sequence.
ACCESSION   DBNGSH010000123 DBNGSH010000000
VERSION     DBNGSH010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4593525"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="AC16CY_02795"
     CDS             <1..573
                     /locus_tag="AC16CY_02795"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR3352735.1"
                     /translation="VISNASCTTNCLAPLAKVIHERFVIRKGWMTTVHAYTNDQQLLD
                     LPHKDLRRARAAALSIIPTTTGAASAVGEVLPSLKGKLDGFAMRVPTPNVSLVDLNVI
                     LDKKTTADEVNQALREAARGSLKGILDVSDEPLVSIDFRGNSHSSIVDSAYTKVMDGD
                     FLKVLSWYDNEWGYSCRCVDLVRMLAARGL"
     gene            638..1828
                     /locus_tag="AC16CY_02800"
     CDS             638..1828
                     /locus_tag="AC16CY_02800"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR3352736.1"
                     /translation="MHKRTIRDLDLSGRRVFVRVDFNVPLKGGVIGDDTRIRESLPTI
                     RYALEAGAACVVLASHLGRPKGKPNPDMSLRPVAVRASQLLGRPVAFADDCVGPAAEA
                     AVRDAPAGGVVLLENLRFHPEEEKNDAGFARGLALLADAYVNDAFGAAHRAHASVEAI
                     VRLVPDSGAGLLMEKELRYLGAAIERPERPFVAVLGGAKVSDKIEVIENLIPRVDRLL
                     VGGAMAYTFFKALGKPVGRSLVEDDKIDSAREILSRSRDRGLTLLLPVDHVVANKVEA
                     GQPTEVLRVDDASIGDRLGLDIGPGTIAAYSAALADAKTVVWNGPMGVFEIDAFAQGT
                     MGVARAVANVRGTTIVGGGDSIAAIRKSGVADRITHISTGGGASLEFLGGRTLPGVAV
                     LPEA"
     gene            complement(1872..2411)
                     /locus_tag="AC16CY_02805"
     CDS             complement(1872..2411)
                     /locus_tag="AC16CY_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3352737.1"
                     /translation="MQRTPTASLARHVLAAGAAAIAVVAMISTAPRAQSSPSAEDISA
                     GMRIYRQKADCQACHGWAADGRKMDSQMPDGANLRVSKLDRAKLVTAIKCGIPGKAMP
                     AFDRLAYSDGRCYGMKQADLQKLGTTMPDPAATLQPREVDLVADFLFAKVIGKGPMNR
                     AACIEYWGSDIETCKDLPN"
     gene            2540..3070
                     /locus_tag="AC16CY_02810"
     CDS             2540..3070
                     /locus_tag="AC16CY_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352738.1"
                     /translation="MKRVEPSLRERAGRMYRRLLLLQRLFQPPRDADEGDPPGTDDAA
                     RDMAVGAAVREVLDELTEHARLLTTIPFPLCEWRPGDAADDQRWRALTEVERREMVSL
                     VAGYDDLITWAETMTSGEMELTALVEVGGGTAQGAASRPSRRTQEAVEYLKAERARIR
                     RFSQDMAFLSRRAGAD"
     gene            3133..>4495
                     /locus_tag="AC16CY_02815"
     CDS             3133..>4495
                     /locus_tag="AC16CY_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3352739.1"
                     /translation="MTTDPRDVIANSPMSRLQIAIIGITIALNALDGFDILSISFASP
                     GIAKEWGIDRAALGIVLSMELIGMAIGSIVLGGVADRIGRRPTVLGCLVVMAGGMFMA
                     TTVNSPVELSIWRVVTGLGIGGMLAAINAVAAEFSSQQKRHLSVSLMSIGYPVGVVLG
                     GMVATQLLKTSDWRSVFYFGSLVTTALIPVVFFTIPESVHWLARKQPAGALEKINRAL
                     ARMGHAAVASLPSITPDVRRRSVTDIFAPGLVAITVIVTLAYFFHITTFYFIMKWVPK
                     IVADFGFPDSSAGGVLVWGNVGGATGGAVFGLLTLRFGVKALTICVMLLSTVMVTLFG
                     QTPPDLGQLSLICAAAGFCTNAAIVGMYAILAQAFPTHVRATGTGFAVGVGRGGSVLA
                     PIIAGFLFTAGYTLPTVAFMMSLGSLVAAGVLSFLKLEGDRAAAERDSRAVATQIPAT
                     GQAR"
BASE COUNT          710 a         1459 c         1521 g          805 t
ORIGIN      
        1 gtcatctcga acgcctcgtg caccaccaac tgcctggcgc cgctcgcgaa ggtgattcac
       61 gagcgcttcg tcatccgcaa ggggtggatg accacggtgc acgcgtatac gaacgaccag
      121 cagctgctcg acttgccgca caaggatctg cgacgcgcac gcgccgcggc actgtcgatc
      181 atcccgacca ccacgggtgc ggcgtccgct gtgggagagg tgctgccctc gctcaagggc
      241 aagctggacg gcttcgccat gagggtgccc acgcccaacg tgtcgctcgt cgatctcaac
      301 gtgatcctgg acaagaagac gacggcggac gaggtgaacc aggcgctgcg tgaggcggcc
      361 aggggatcgc tcaagggcat tctcgacgtg tcggacgagc cgctcgtgtc gatcgacttc
      421 aggggcaact cccactcgtc gatcgtggac tcggcctaca cgaaggtcat ggacggcgac
      481 tttctgaagg tcctgtcctg gtacgacaac gagtggggct actcgtgccg ctgcgtggat
      541 ctggttcgaa tgctcgctgc ccgcggactg tgatcagggg tcataacttg atttttgcac
      601 tcctgcactc ccgtgcaaaa atcaagatct gaccccattg cataaacgga caatccgcga
      661 tctcgatctg agcggccggc gcgtgttcgt gcgcgtcgac ttcaatgtcc ccctgaaagg
      721 gggcgtcatc ggcgacgaca cgcgcatacg cgagtcgctg ccaacaatcc ggtacgcgct
      781 ggaggccggc gcggcgtgcg tcgtcctcgc ctcccatctc gggcgcccga agggcaagcc
      841 aaaccccgac atgagcctgc ggccggtggc ggtccgtgcc agccagttgc tcggccgccc
      901 ggtggcgttc gccgacgact gcgtgggtcc ggcggccgag gcagccgtac gggacgcgcc
      961 ggcgggcggc gtcgtgctgc tcgagaacct gcgctttcac cccgaggaag agaagaacga
     1021 cgccggattc gccagggggc tcgcgttgct cgccgacgcg tacgtgaacg acgcgtttgg
     1081 agccgcccac agagcgcacg cctcggtgga ggccatcgtt cgtctggtgc ctgattccgg
     1141 cgccggtctc ctgatggaaa aggagctgcg gtatctcggc gcggcgatcg agcgtccaga
     1201 gcggccgttt gtcgccgtgc tcggcggcgc caaggtctcg gacaagatcg aggtgatcga
     1261 gaatctcatc ccgcgcgtcg atcggctgct cgtcggcggc gccatggcct acaccttctt
     1321 caaggccttg gggaaaccgg tcggccgctc actcgtggaa gacgacaaga tcgactcggc
     1381 gcgcgagatc ctgtcgcgtt cgcgcgaccg cggcctcacg cttcttctcc ccgtcgatca
     1441 tgtcgtggcg aacaaggtcg aggcaggaca gcccacggaa gtgctgcgcg tggacgatgc
     1501 ctccatcgga gaccggctcg ggctcgacat cggccccggg acgatcgccg cgtactcggc
     1561 cgcgcttgca gacgcgaaga cggtcgtctg gaacggcccc atgggcgtgt tcgaaatcga
     1621 cgcgttcgct cagggcacga tgggcgtggc tcgtgccgtg gcaaacgtgc gcggcacgac
     1681 catcgtcggc ggcggcgatt cgatcgcggc catcaggaag tcgggagtgg ccgaccgcat
     1741 cacgcacatt tcgaccggcg gaggggcgtc gctggagttt ctcgggggcc gtacgctgcc
     1801 gggagtcgcg gtcctacctg aggcctgagc cctgggccct gaggcctaaa acctgggccc
     1861 tgaggcctga gtcagttcgg cagatcctta cacgtctcga tatccgagcc ccagtactcg
     1921 atgcaggcgg cacggttcat cggacccttg ccgatgacct ttgcgaacag gaagtcggcc
     1981 acgagatcga cctcgcgcgg ctgcaacgtt gccgcggggt cgggcatcgt ggtgccgagc
     2041 ttctgcaggt cggcctgctt catcccgtag cagcgtccgt cgctgtaggc aagccggtcg
     2101 aaggccggca tcgcctttcc gggaatgccg cacttgattg cggtcacgag cttggcgcga
     2161 tcgagcttgc tgacgcgcag gttggcgccg tctggcatct ggctgtccat ctttctgccg
     2221 tcggccgccc acccgtggca ggcctggcaa tcggccttct ggcggtagat gcgcatcccg
     2281 gctgaaatgt cctccgcgct cggcgagctc tgggcccggg gcgccgtcga gatcatcgcg
     2341 acgacggcga tggctgcggc gccggccgcg agcacgtgac gcgcgagcga ggctgtgggc
     2401 gtcctttgca cacgatcatg ttaacttcag cccgtttcgc cacggccgag ctataatccg
     2461 cacgaccccc aggcgcgtcg gacccatcaa ccgaaatttc gcggcaaacg cacgatcgct
     2521 cgtcagatcg gggtgcgcca tgaagcgggt ggagccgtct ctcagagaac gcgcgggccg
     2581 catgtaccgg cggctgctgc tgctccagcg tctgttccaa ccaccaaggg acgccgacga
     2641 gggcgacccg ccaggcaccg acgacgccgc gcgtgacatg gcggtgggcg ccgcggtgcg
     2701 cgaggtgctc gacgagctga ccgagcacgc gcggctgctg acgacgatcc cgtttccctt
     2761 gtgcgaatgg cggcccggcg acgccgccga cgatcaacga tggcgcgcgc tcacggaggt
     2821 cgaacgccgc gagatggtgt cgctcgtcgc aggctacgac gacctgatca cgtgggccga
     2881 gacgatgacg agcggagaga tggagctgac tgcgctcgtc gaagtgggtg gcggaaccgc
     2941 gcagggagcc gcgagccgcc cgtcacgccg gacgcaggag gctgtcgagt acctgaaggc
     3001 ggaacgggcg cggatccgcc gcttcagcca ggacatggcc tttctgagcc gccgggccgg
     3061 cgccgactga ccccgtttca agttttgagt tttgagtttt gagttttgag ttgttgacga
     3121 tactctcacg ccatgacgac tgacccgcga gacgtgatcg ccaactcgcc gatgagccga
     3181 ttgcagatag ccatcatcgg catcaccatc gccctcaacg cgctcgacgg cttcgacatt
     3241 ctctcgatca gctttgcgtc gcccggcatc gccaaagagt ggggcatcga ccgggccgcg
     3301 cttggcatcg tgctgtcgat ggagctcatc ggcatggcga tcggctcgat cgtgctcgga
     3361 ggcgtggccg accggatcgg gcggcgcccg acggtcctcg gctgtctcgt ggtgatggcg
     3421 ggcggcatgt tcatggccac gacggtgaac agcccggtcg agctgtcgat ctggagggtc
     3481 gtgaccggtc tcggcatcgg cggcatgctc gcggcgatca acgctgtcgc ggcggagttc
     3541 tcgagccagc agaaacggca tctgagcgtg tcgctgatgt cgatcggcta tccggtcggc
     3601 gtcgtgctcg gcggcatggt ggcgacacag ctgctcaaga cgagcgactg gcgttcggtg
     3661 ttctacttcg gctccctcgt gacgacagcg ctgattcccg tcgtgttctt caccataccc
     3721 gagtcggtgc actggctggc gcgcaaacag ccggccgggg cgctcgaaaa gatcaatcgc
     3781 gcgctggcgc gcatggggca cgccgcggtc gcctcgctgc cgtcgattac ccccgacgtc
     3841 cgtaggcgat cggtcaccga catcttcgcg ccgggtctcg tcgcgatcac ggtgatcgtc
     3901 accctggcct acttcttcca catcacgacg ttctacttca tcatgaagtg ggtgccgaag
     3961 atcgtcgctg atttcggctt ccccgattcg tccgcgggcg gagtgctcgt ctgggggaac
     4021 gtcggcgggg ccacgggcgg agcggtgttc ggactcctga cgctgcgctt cggggtcaag
     4081 gcgctgacga tctgcgtgat gctgttgtcc accgtcatgg tgacgctgtt cggacaaacc
     4141 ccgcccgatc ttggacagtt gtcgctcatc tgcgccgccg ccggcttctg caccaacgcg
     4201 gccatcgtcg ggatgtacgc gattctcgcg caggcgttcc ccacgcacgt gcgtgcgaca
     4261 ggtaccggct tcgccgtcgg cgtcgggcgt ggaggctcgg tgctggcgcc gatcatcgcg
     4321 gggttcctgt tcaccgccgg atacacgctt cccaccgtgg ccttcatgat gtcgctcggc
     4381 tcgctcgtgg cggctggagt gctctcgttt ctcaagctcg agggagaccg ggcagcggcc
     4441 gaacgcgact cgcgcgcggt ggcgacgcag attccggcga ccggccaggc gcgct
//
LOCUS       DBNGSH010000124        10143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3404665, whole genome shotgun sequence.
ACCESSION   DBNGSH010000124 DBNGSH010000000
VERSION     DBNGSH010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3404665"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /gene="leuS"
                     /locus_tag="AC16CY_02820"
     CDS             complement(<1..1772)
                     /gene="leuS"
                     /locus_tag="AC16CY_02820"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3352740.1"
                     /translation="MPDYHPQDLDRKWQLRWAESRAFEVSADPSRPKFYCLEMFAYPS
                     GHAHVGHLRNYIIGDVVARTKWMRGYNVLHPFGWDAFGLPAENAAIKSGIHPEVSTRD
                     NIAHMKGQLQRLGISYAWERELATCDAEYYKWNQWLFIRMFERGLAFRKKSSVNWCPV
                     DKTVLANEQVVDGGCWRCGTPVETRDLEQWFFKITAYADELLDATKHLTRWPEKVLTM
                     QQNWIGRSEGAYVKFPLDPESGVPSAGHIEVFTTRIDTIFGATFVLLGPEHPLVAQFA
                     ERSKDPAGFKRQAHAFRMQDRAARMSGDIEKQGFDTGFRATNPFTGQAVPIWVANFVL
                     GEYGTGAVMAVPAHDQRDFEFARKFNLPIRVVVTPDGRSAASDSMTEAAPAYGTLVDS
                     GEFSGLGSEDAQKRMTEWALGRGIGEGTVQFRLKDWGISRQRYWGTPIPMIYCDRDGI
                     VAVPDDQLPVRLPPLAQFTGRGDSPLAQVPEFVNVTCPRCGGPARRETDTMDTFVDSS
                     WYFYRFADPRNDAKPADPAALKYWLPVDFYVGGVEHAILHLIYSRFFARVFRDIGLVD
                     HSEPFTQLLTQGMVLKDGAVMSKS"
     gene            complement(1831..2856)
                     /locus_tag="AC16CY_02825"
     CDS             complement(1831..2856)
                     /locus_tag="AC16CY_02825"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3352741.1"
                     /translation="MSLTLFTSPIFADHLTPPGHPERVERFEVMQVVAAEFRQRGVAV
                     ETPRPATDNEIARIHERDYITLIRETAGRATALDPDTFTSPHTWEAACLAAGAAVGAV
                     EHVLEAGDGARAFALVRPPGHHAERNRAMGFCFFNSIAIAAAHARARGLSRVAIVDFD
                     VHHGNGTQWSFYDDPTVLFVSSHQYPYYPGTGAAQDIGRGAGTGFTVNLPLAAGATDA
                     DYELAYSKLALPVVAQFRPELVLVSAGYDAHMDDPLAGMRLTAPYFGRLMQAIAGVAD
                     ECCKGRLVAITEGGYDLAGLAASLRTSLRALLGESAGDVRPDGSRSRGEAAIAAVTPH
                     LSKYWKL"
     gene            2871..3350
                     /locus_tag="AC16CY_02830"
     CDS             2871..3350
                     /locus_tag="AC16CY_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HIR3352742.1"
                     /translation="MTILLWILAVALVVVGLVGVVMPAMPGHVLILAGLVVGAWADGF
                     TRVGVWTLVLIGAISLACYLVDFAAAALGARHLGASTRAMSGAALGTLFGFFLGLPGL
                     IIGPFLGAVLGELTVTNDFARAGKVGVAAWIGFAIGAAVKVGMAFLMIAIFVAAFVF"
     gene            3475..4851
                     /locus_tag="AC16CY_02835"
     CDS             3475..4851
                     /locus_tag="AC16CY_02835"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/sorbosone family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HIR3352743.1"
                     /translation="MRRLTAAVTTILASVCMLPAAQDPTIVTNPPESFEMRVVARGLD
                     GPWELAWGPDQQLWVTERRGRRIVRVNPADGSRTTLVSIPEAHQSVGQDGLLGMALHP
                     DLLRGSGTDYVFVAFTYDDAPGPELVRKMAIRRYQLDQRARALTGPTDVMTGLPAHDD
                     HLGGRLIIGADRKLYLSIGDQGSNFGSNRCNANHAQDLPTSAQVRAKDWSSYQGKILR
                     VELDGAIPADNPEIDGVRSHVFSYGHRNPLGLVSGGGLIYETEHGPNTDDEINLIEAG
                     RNYGWPNVAGYRDDKSYTYVNWSTSTPRPCSALLPAGAVPPSVLTQSETAWNHPRFAP
                     PLRTFFTVENGFDTSAIGSGTIAPGGIDLYTSDRVPGWRNSLLALSLIRGVVYRLRLA
                     ADGRSISAPPAEMFRTANRYRDIALNPDGRTIYMATDVDGPSRDASGAPRSLSNPGSI
                     LEFTYMGK"
     gene            4854..6506
                     /locus_tag="AC16CY_02840"
     CDS             4854..6506
                     /locus_tag="AC16CY_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3352744.1"
                     /translation="MSRGLRMSRPVLGPWLAAAFLIASTAMFGAQTPQDRLQPLKRDV
                     VAEVDARRQLTQQIVDTLFSFSELGFQEIETERYLTGILEREGFTVRRGLAGIPTAWT
                     ATWGSGRPVIALGTDVDGVPGTNQRPGVLTRSEIVPGAPGHGEGHNSGQAVVVTAVLA
                     AKRVMEREKLSGTLVVWPGVAEEQLATKAHLVRAGVFKDVDVVLYSHVGAALATSWGD
                     TSSSGLVSVQYTFAGSSSHAAIAPWLGRSALDAVELMNIGWNFRREHLRLAQRSHYVI
                     TNGGDQPNVVPANASVWYYFRENDYDRIKELWDLGDTMARGAATMTATTVESRVLGSA
                     WPLHGNRPVAEALQANIASVGVPPWSDADQQFARALQRSLGVPERGLVSSVAPVVSGV
                     ELIPDNTKTGGSSDDVGDIMWSVPTATLNFPSNVGGTIQHHWSSAIAMATPVAHKGAT
                     QGAKVQAMTVLDLALRPDLVKAARDYFDTVQGKQRKYQPLLRPGDQPAVWLNKESMER
                     FRPELRKHYYDSTRFASYLDQLSVPYPPAMPASPPAAPPAGR"
     gene            complement(6513..7472)
                     /locus_tag="AC16CY_02845"
     CDS             complement(6513..7472)
                     /locus_tag="AC16CY_02845"
                     /inference="COORDINATES: protein motif:HMM:NF028479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HIR3352745.1"
                     /translation="MRRASAGVVIVLLGASVLAQRPSDPALLVPEAAPELDYVLAPTP
                     VALPAGVTMGATASVAFDARGHLYVLTRGATAFFEFDQNGAFVRSFGDKMFTRSHGLR
                     IDGDGNLWATDVGAHVVVKLNPKGEVLLTLGTRGEAGEWNEAAGSKKLNQPNDVAIAT
                     NGDVFVVQGHTPGPNGDARVLKFDKSGRFVKSWGGKGSGPGQFQVAHGIDIDAKGLLW
                     VADRENQRVQVFDAEGTFVREIKYKGLPCSLDIGRQYIYMVNGFAGQVLRMDLNGKVL
                     AALGKPGKGPGEFGEAHMIAVSPKDELYVADSVNAALVKFVKK"
     gene            complement(7525..10026)
                     /locus_tag="AC16CY_02850"
     CDS             complement(7525..10026)
                     /locus_tag="AC16CY_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352746.1"
                     /translation="MRLHQFVDELQRDVRGALRQMRAAPGFTAVAALTLALGIGANSA
                     IFALADAVLLRPLPYANGDRLVFIDEHGPQQGGRSRIELLNFDEWMAQNRTFETMAAI
                     WIPAAGGGPTLMGTDGTPELLSSQTVTPLYFDVFGVTPILGRTFRSDDPIVQPDAVVL
                     SESFWHRRFAGDPSIIGRAISFEGGNALTVIGIVPSAFQFRPAVFRDDDVVPKPVSVW
                     VLLPRPRADVEDTTRGQCGVCRFLQVVGRLKPGTSIEAARADLTGLAETLAARTGARS
                     PRRITTTSMRELLIGRELRLTSILFLGVVGFVLLLCCANVANLVLARATVRTRELAVR
                     AALGAGRRRIVAQLMTESLALAAAGGVLGIAFGAAILAVAPSLLPAELLPSVIELGVD
                     GRVVAFCVAMTCLTGVLFGLAPAWQATSVSLLEAMTSTGRTATGRGGQFRRVLAAAEV
                     AAAVLVLCGAGLLLRTLLAIDSSDQGYRAENERVLTLDFVLPGNRYATADSRRQFYDG
                     VEREVEAVPGVRSAGWATSLPLGGSQLGGQSFEIIGDPPAPDGDSSSADFQIVSHTYF
                     RTLDLPIVAGRAFTAEDRADASPVCIVSEGFVRRYLQGRNPLGIRLLVNASRDAAPRE
                     IVGVARQVKERPDETEDLIQIYVPNQQVPHTEAFLVVRTEGEAKAQAPDIRRAIARVD
                     RQLAVGTVVTLEGIARQATGRHRFRAMLVLTFAALALVLAMVGVFGVLAYSVQQRVRE
                     FGVRIALGASTRQVLGLVLGGAARVVGAGAVIGLIAAAALSRSISAFLFGVEPLDPVT
                     FGSVAIVLALTAALASAAPALRAARVDPVVAFRSE"
BASE COUNT         1717 a         3496 c         3319 g         1611 t
ORIGIN      
        1 ttcgacttcg acatgaccgc gccgtccttc agcaccatgc cctgcgtgag cagttgggtg
       61 aacggttcgg agtgatccac gaggccgatg tcccggaaca cgcgcgcgaa gaaccgcgag
      121 tagatcaggt gaaggatggc gtgctcgacg ccgcccacgt agaagtcgac tggcagccaa
      181 tacttcagcg ccgccggatc ggccggcttc gcatcgttgc ggggatcggc aaaccggtag
      241 aagtaccacg acgaatcgac gaaggtgtcc atcgtgtccg tctcgcggcg cgcgggacca
      301 ccgcaccggg ggcacgtgac gttgacgaac tcaggcacct gggccagcgg cgagtcgccc
      361 cggccggtga actgggcgag cggcggcaac cgtacgggaa gctggtcgtc gggaacggca
      421 acgatgccgt ccctgtcgca gtagatcatc gggatcggcg tgccccagta ccgctggcgc
      481 gagatgcccc agtccttgag ccggaattga accgtcccct cgccgatacc gcggcccagc
      541 gcccactccg tcatccgctt ctgcgcgtcc tcggagccca gcccgctgaa ctcccccgag
      601 tccacgagcg tgccgtaggc cggtgcggcc tctgtcatcg agtccgacgc cgcgcttcgg
      661 ccgtcaggcg tcacgacgac gcgaatgggc aggttgaact tgcgggcgaa ctcgaagtcg
      721 cgctgatcgt gcgcgggaac cgccatcacc gcgccggtgc cgtactcacc gagcacgaag
      781 ttcgcgaccc agatgggcac tgcctggccg gtgaacgggt tcgtcgctcg aaacccggtg
      841 tcgaacccct gcttctcgat gtccccgctc atgcgcgcgg cgcgatcctg catcctgaag
      901 gcatgggcct ggcgcttgaa cccggctgga tccttcgacc gctcggcaaa ctgcgccacc
      961 agcgggtgtt ccggcccgag cagcacgaag gtggcgccga agatcgtgtc gatgcgcgtc
     1021 gtgaacacct cgatgtggcc ggcgctcggg accccggatt cgggatcgag cggaaacttc
     1081 acataggcgc cctccgaccg cccgatccag ttctgctgca tggtcagaac cttctcgggc
     1141 catctggtga gatgcttcgt cgcgtccagt aattcgtcgg cgtacgccgt gatcttgaag
     1201 aaccactgct cgaggtcgcg cgtctccacc ggcgtgccgc aacgccagca gccgccgtcc
     1261 acgacctgct cgttggcaag caccgtcttg tccacggggc accagttcac cgacgacttc
     1321 ttccggaacg cgaggcctcg ctcgaacatc cggatgaaca gccactggtt ccacttgtag
     1381 tactcggcgt cgcacgtggc cagctcgcgt tcccaggcat agctgatgcc caggcgctga
     1441 agctgcccct tcatgtgcgc gatgttgtct cgcgtggaga cctcggggtg gatgccgctt
     1501 ttgatggcgg cgttctcggc gggcaggccg aaggcgtccc agccgaacgg atgcaagacg
     1561 ttgtagccgc gcatccactt ggtccgcgcc accacgtcgc cgatgatgta gttgcgcagg
     1621 tgcccgacgt gcgcgtgccc cgacggatag gcaaacatct ccaggcagta gaacttcggg
     1681 cgcgacgggt cggcgctcac ctcgaacgcg cgcgattccg cccaccgcag ttgccacttc
     1741 cgatcgagat cctgcggatg gtagtccggc atgaaatggg gtcgggagtc atttattgaa
     1801 agacgctttc ataaatgact cccgacccca ttatagcttc cagtatttcg agagatgcgg
     1861 cgtgaccgcc gcaatcgccg cctcgccgcg cgagcgcgag ccgtcgggcc gaacgtctcc
     1921 ggcgctctca cccaggagcg cacgaagaga cgttcgaagg ctcgcggcca gccccgcgag
     1981 gtcgtaaccg ccttcagtga tcgcgacgag acggcctttg cagcactcgt cggcgacgcc
     2041 ggcgattgcc tgcatcaggc ggccgaagta gggcgcggtc agccgcatcc cggccagcgg
     2101 atcgtccatg tgcgcatcgt agccagccga cacgagcacc agttctggcc ggaactgcgc
     2161 gacgacgggc agagcgagct tcgagtacgc gagctcgtag tccgcatcgg tggcgcccgc
     2221 cgcaagcgga aggttcaccg tgaaaccagt cccggcaccc cggccgatgt cctgagccgc
     2281 gccggtgccg ggatagtacg gatactggtg cgaggagacg aagaggaccg tgggatcgtc
     2341 gtagaagctc cactgcgtgc cgttgccgtg gtgcacgtcg aaatccacga tggcgacgcg
     2401 cgagagtccg cgggcacggg cgtgtgccgc ggcgatcgcg atgctgttga agaagcagaa
     2461 gcccatcgcc cggttgcgtt cggcgtgatg ccccggcgga cgaacgagcg cgaaggcgcg
     2521 agccccgtcg ccagcctcga gcacatgttc cacggcgccc acggctgcgc cggcagcgag
     2581 acaggcggcc tcccacgtat gaggcgacgt gaacgtgtcc ggatcgagcg cggtggcccg
     2641 gcccgctgtc tcgcggatca gtgtgatgta gtctcgttcg tggatcctcg caatctcgtt
     2701 atcggtcgcc ggccgtggcg tctcgacggc cacgccgcgc tgccggaact cggcggccac
     2761 gacctgcatc acctcgaagc gttcgacacg ctcggggtgc ccgggagggg tcaggtggtc
     2821 ggcgaaaatc ggcgaggtga agagcgtcag cgacacggga gaattgtacg gtgaccatcc
     2881 tgctctggat actggccgtc gcgctcgttg tcgtcggcct cgtcggcgtc gtgatgccgg
     2941 cgatgcccgg ccatgttctc atcctggccg gcctcgtcgt cggggcctgg gcggacggat
     3001 tcacacgcgt cggcgtctgg acgctcgtcc tcatcggggc catctcgctc gcctgctacc
     3061 tcgtggactt cgcggcggca gccctgggcg cgcgccatct gggcgcgagc acgcgcgcca
     3121 tgagcggcgc cgcgctcgga accctgttcg gcttcttcct gggcctgcct gggctcatca
     3181 tcggcccctt cctcggcgcc gtgctgggcg agctgacggt gaccaacgac ttcgccaggg
     3241 ccggcaaggt cggcgtggcg gcctggatcg gcttcgcgat cggcgcggcc gtcaaagtcg
     3301 gcatggcgtt cctcatgatc gccatcttcg tcgcggcctt cgtgttttga ctttcgaact
     3361 cgcttgtgtc ggtaatgttg ccaacgatcg ttgcttgcag catggaccgc ttggtcagcg
     3421 tggcctcggc cggagccgac caacacaagc gagctgtggt gtaatgggcg cattatgcgc
     3481 agactgacgg cggcagttac cacgatcctg gcctccgtct gcatgctgcc ggctgcgcag
     3541 gatccgacga tcgtcacgaa ccctcccgag tcgttcgaga tgcgtgtggt cgcgagaggg
     3601 ctggacggcc cctgggagct cgcgtgggga ccggatcagc agttgtgggt cacggagcgg
     3661 cgtggccggc gcatcgttcg ggtcaacccg gccgacggct cgaggacgac gcttgtctcg
     3721 atacccgaag cgcaccagag cgtcggacag gacgggctgc tcggcatggc cctccacccc
     3781 gatttgcttc gcggcagcgg caccgactac gtcttcgtcg ccttcaccta cgacgacgcc
     3841 ccaggaccgg aactggtgag gaagatggcg atccgccgtt atcagctcga ccagcgtgcg
     3901 cgcgcgctga ccggtccgac ggatgtgatg accgggcttc ccgcccacga cgatcatctc
     3961 ggcgggcgcc tgatcatcgg cgccgatcgc aaactgtatc tttcgatcgg cgatcagggg
     4021 agcaacttcg ggagcaaccg gtgcaacgcc aaccacgccc aggatctgcc gacgtctgcc
     4081 caggtgcggg cgaaggactg gtcgagctat cagggcaaaa tcctgcgcgt ggagctcgac
     4141 ggggcgatcc cggccgacaa cccggagatc gacggcgtgc gcagccacgt cttctcgtac
     4201 ggccaccgca acccgcttgg cctcgtgtct ggtggaggct tgatctacga gaccgagcac
     4261 gggccgaaca ccgacgacga aatcaatctc atcgaggccg gacgcaatta cggctggccg
     4321 aacgtcgctg gataccgcga cgacaagagc tacacctacg tcaactggtc aacttccacg
     4381 ccgcggccgt gcagcgcgct gctgcctgcc ggcgccgttc ctccttcggt gctgacacag
     4441 tcggagacgg cctggaacca cccgcgcttt gctcccccgc tgcgcacgtt cttcacggtc
     4501 gagaacggct tcgacaccag tgcgatcggc agcggcacca tcgctccggg cggcatcgat
     4561 ctctacacgt ccgatcgagt gccaggctgg cgaaactcgc tgctcgcgct cagtctgatc
     4621 aggggcgtgg tgtatcggct gcgtcttgct gcggacggac gctccatcag cgccccgccg
     4681 gccgagatgt tccggacggc aaaccggtac cgggacatcg ccctcaaccc tgacgggcgc
     4741 acgatctaca tggcgaccga cgtggacggt ccaagccggg acgcgtcggg cgcgccgcgc
     4801 agcctgtcca acccggggag cattctcgag ttcacctaca tggggaaatg accatgagtc
     4861 gcggacttcg catgtcgcgg ccggtgctcg gaccatggct cgccgcggcg ttccttatcg
     4921 catcgaccgc catgttcgga gctcagacgc cccaagaccg gctgcagccg cttaaacgcg
     4981 acgtcgtcgc cgaagtggac gcccgtcgtc agttgacgca gcagatcgtg gacacgctgt
     5041 tcagcttcag cgagctcggg tttcaggaaa tcgagaccga acggtacctg accggcattc
     5101 tcgagcggga agggttcacc gtgcgacgcg ggcttgccgg cattcccacc gcgtggacgg
     5161 ccacgtgggg atcgggccgg ccggtcatcg cgctcggcac cgacgtggac ggcgtcccag
     5221 gcacgaacca gcggcctggc gtcctgactc gatccgagat cgtccccggc gcgcccgggc
     5281 acggagaagg acataactcg ggccaggcgg tcgtggtcac ggccgtcctc gccgccaagc
     5341 gcgtcatgga gcgcgagaag ctttccggca cgctcgtcgt ctggccaggc gtcgccgaag
     5401 aacagctcgc gacgaaggcg cacctggtgc gggcaggcgt cttcaaggac gtggacgtcg
     5461 tgttgtactc gcacgtcggt gccgcgctcg ccacgtcctg gggcgacacc agcagcagcg
     5521 ggctcgtctc ggtccagtac actttcgcgg gctcgagctc gcatgcggcc atcgcgccgt
     5581 ggctgggccg cagcgcgctc gacgcggtcg agctgatgaa catcggctgg aacttcaggc
     5641 gcgagcacct caggctggct caacgatcgc actacgtcat cacaaacggc ggcgatcagc
     5701 cgaacgtcgt gcccgcaaac gcgtccgtct ggtactactt ccgcgagaac gactacgacc
     5761 gcatcaagga gctgtgggac ctcggcgaca cgatggccag gggcgccgcg acgatgacgg
     5821 ccacgactgt cgaatcgcgt gtgctcggct cggcatggcc gctccacggc aaccgtcccg
     5881 tggcggaggc gctgcaggcc aacatcgcat cggtcggggt tcccccctgg agcgacgcgg
     5941 accagcagtt cgcgcgcgcg ctccagcggt ctctgggcgt acccgagcga ggcttggtct
     6001 cgtctgtcgc gcccgtcgtg tcaggcgtcg agctcattcc cgacaacacg aagacgggcg
     6061 gctcctccga tgacgtcggg gacatcatgt ggagcgtgcc gacggccacg ctgaactttc
     6121 cgtcgaacgt cggcgggacg atccagcatc actggtccag cgcgattgcg atggccaccc
     6181 ccgtcgccca caagggcgcg acgcaggggg ccaaagtgca ggcgatgacc gttctcgatc
     6241 tggcattgcg gcccgatctc gtcaaggccg cgcgagacta cttcgacacc gtgcaaggca
     6301 aacagcggaa atatcagccg ctgcttcgcc ccggcgacca gccggccgtg tggctcaaca
     6361 aggaaagcat ggagcggttc cggcccgagc tccggaagca ctattacgac tcaaccaggt
     6421 ttgcgtcgta cctcgatcag ttgagcgtgc cgtatccgcc ggcgatgccc gcatcgccgc
     6481 cagcagcgcc gccggccggg cgctgacgaa cgctacttct ttacgaactt cacgagagcg
     6541 gcgttcaccg agtcggccac gtacagctcg tctttcgggc tgacggcaat catgtgcgct
     6601 tcgccgaact ccccgggacc cttgcccggc ttgcccagcg ccgccagcac cttgccgttc
     6661 aggtccatcc tgaggacctg ccccgcgaat ccgttcacca tgtagatgta ctggcgaccg
     6721 atgtcgaggc tgcacggcag ccccttgtat ttgatctccc tgacgaacgt accctcggcg
     6781 tcgaagacct gcacccgctg gttctcgcgg tcggccaccc agagcagacc cttggcgtcg
     6841 atgtcgatgc catgcgcaac ctggaactgc ccggggcccg agccttttcc gccccacgac
     6901 ttgacgaagc gcccgctctt gtcgaacttc agcacccgcg cgtcgccgtt gggacccggc
     6961 gtgtgaccct gcaccacgaa gacgtctccg tttgtcgcaa tggcgacgtc gttcggctgg
     7021 ttgagcttct tcgagccggc cgcctcgttc cactcgcccg cctcgcctct cgtcccgagc
     7081 gtcaaaagga cctcgccctt tggattcagc ttcaccacga catgggcgcc gacgtcggtc
     7141 gcccagagat tgccgtctcc gtcgatgcga aggccgtgcg accgggtgaa catcttgtcg
     7201 ccgaaagagc gcacgaacgc gccgttctga tcgaactcga agaaggctgt cgcgccgcgg
     7261 gtgaggacgt acaggtgacc gcgcgcgtcg aacgccacgc tcgccgtcgc gcccatcgtg
     7321 acgcccgcgg gaagcgcgac tggtgtcggc gccaggacat agtccagttc gggggctgct
     7381 tccggcacca gcaacgccgg atccgacggc cgctgcgcga ggacggacgc gccgagcagg
     7441 acgatgacga ctccggcaga agcccttctc atatgcccct ccatgagatg ggccgaagtc
     7501 tacctgaaac gacgacggcg tacgctactc gctccgaaac gccaccacgg gatcgacacg
     7561 cgcggcgcgg agggctggcg ccgccgacgc gagggcggca gtcagtgcga gcacaatcgc
     7621 caccgatccg aacgtcactg gatcgagcgg ctcgacgccg aacagaaacg ccgaaatcga
     7681 gcggctgagg gccgccgcgg caatcaagcc gatgaccgcc ccggcgccga cgacacgcgc
     7741 ggcgccgccc aggacgagcc cgagcacctg acgtgtactg gcgccgagtg cgatgcggac
     7801 gccgaactcg cgcacgcgct gctgcaccga ataggcgagc accccaaata cacctaccat
     7861 cgccagcacg agggcgagcg cagcgaaggt cagcacgagc atggcgcgga accggtggcg
     7921 gccggtcgcc tgccgcgcga tgccttcgag cgtgacgacg gtgccgacgg cgagctgcct
     7981 gtccacgcgc gcgatggcac ggcggatgtc cggcgcctgc gccttggcct cgccctcggt
     8041 gcgcacgacc aggaacgcct cggtgtgcgg cacctgctgg ttgggaacgt aaatctgaat
     8101 gagatcctcg gtttcgtccg ggcgttcctt cacctgccgg gcgacaccga cgatctcgcg
     8161 cggggccgca tcacggctcg cgttcaccag aagccggatc ccgaggggat tcctgccctg
     8221 taggtaccgc ctcacgaagc cctcgctcac gatgcacacg ggcgatgcgt cggcgcggtc
     8281 ctcagccgtg aaagcccggc ctgcaacgat cggaagatcg agcgtgcgga agtacgtgtg
     8341 gctcacgatc tgaaagtccg ccgacgagct gtcgccgtcg ggtgccggcg gatctccgat
     8401 gatctcgaac gactgaccgc cgagctgcga cccgccaagc ggcagcgacg tcgcccaccc
     8461 tgcgcttcgt acgccgggaa ccgcctccac ctctcgctcg acgccatcgt agaactgccg
     8521 ccgcgagtcg gcggtggcgt accggttccc cggcaggacg aagtcgagtg tcagcacccg
     8581 ctcgttctcg gcccggtagc cctggtccga gctgtcgatg gccagcagcg tccgcagcag
     8641 caggccggcg ccgcagagca cgagcacggc tgccgccacc tccgctgccg ccagcacgcg
     8701 ccggaactgc ccgccgcggc cggtggcagt ccgccctgtg gacgtcatcg cctccagcag
     8761 cgacacgctg gtcgcctgcc aggctggcgc cagtccgaac agcactccgg tcaggcaggt
     8821 cattgccacg cagaacgcca cgacacggcc gtccacgccg agctcgatga cgcttggcaa
     8881 cagctccgcg ggcaggagcg acggcgccac agccagaatt gcggcaccga aagcgatgcc
     8941 aagcacaccg ccggccgcgg ccagcgccag actctcggtc atcaattgcg cgacgatccg
     9001 ccgccgccct gcgccaagcg ccgcgcgcac cgccagctcc cgcgtccgca ctgttgcgcg
     9061 cgcgagcacg agattggcaa cgttcgcgca gcacaggagc agcacgaagc cgacgacgcc
     9121 gaggaacaga atcgaggtga gccgcagctc ccggccgatc agcagctcgc gcatcgacgt
     9181 cgtcgtgata cggcgaggcg atcgcgcgcc ggtccgcgcg gcgagcgtct cggccagccc
     9241 tgtgagatca gcacgggccg cctcgatgga cgtgccaggc ttcaggcggc cgacgacctg
     9301 caggaatcgg cagacgccgc actgccctcg cgttgtgtct tcgacgtccg cacgaggccg
     9361 cggcagcagc acccacacgc tcaccggctt cgggaccaca tcgtcgtccc ggaagaccgc
     9421 gggccggaac tggaacgccg acggcacgat gccgatgacg gtcaacgcgt tcccaccctc
     9481 gaacgagatg gcgcgcccga tgatggacgg atcgccggcg aagcgccggt gccagaaact
     9541 ctcgctcagc acgacggcgt cgggctgcac gatgggatcg tcagagcgaa aggtgcgccc
     9601 gaggatcggc gtgacgccga agacgtcgaa gtacagcggc gtcacggtct gcgacgagag
     9661 cagctccggt gtgccgtcgg tgcccatcaa cgtcggccct ccgccggcgg cgggaatcca
     9721 gattgccgcc atcgtctcga aggtgcggtt ctgcgccatc cactcgtcga agttcagcag
     9781 ctcgatgcgg ctccggccgc cttgctgcgg gccgtgctcg tcgatgaaca ccaggcggtc
     9841 gccgttggca tatggcagcg gacgcagcag taccgcatcg gccagcgcga agatcgcgct
     9901 attggcgccg atgcccagcg ccagcgtcag cgcggcgacc gctgtaaagc caggcgccgc
     9961 acgcatctgg cgcagcgcgc ccctgacgtc ccgctgcagc tcgtcgacga attggtgcag
    10021 ccgcatctcg cgctctcgct ttcgtccaag gtctacacag gttcgtttga gctgtccgag
    10081 atcgccgatg cgcgccagga cgatctcgcg agcggtctgc ggatccacgc cgcgctcgac
    10141 cag
//
LOCUS       DBNGSH010000125         1632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2216254, whole genome shotgun sequence.
ACCESSION   DBNGSH010000125 DBNGSH010000000
VERSION     DBNGSH010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2216254"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="AC16CY_02855"
     CDS             complement(<1..323)
                     /locus_tag="AC16CY_02855"
                     /inference="COORDINATES: protein motif:HMM:NF016355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding YajQ family protein"
                     /protein_id="HIR3352747.1"
                     /translation="MQCSFDITSNVDLQEVDNALNQARKEVAQRYDFKGSKASIEFDP
                     NESRLVLVADDEFKLNSLWEIVQTRLVRRNVPVRNLTRGPATGAASGTVRQEISLQQG
                     IPTEKA"
     gene            complement(336..>1632)
                     /locus_tag="AC16CY_02860"
     CDS             complement(336..>1632)
                     /locus_tag="AC16CY_02860"
                     /inference="COORDINATES: protein motif:HMM:NF048511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HIR3352748.1"
                     /translation="LVSWHGIGSARRGLEALLDAVEQLQGAPLPFSVLERDVLSARVA
                     DYEPGMLDTLIAAGEVVWSGVEPRDGRIALYLTDHFARLGPSGSPATIEGRAGDILTY
                     LRSHGASFFGAVHEGTGGGFPGETVDALWDLVWKGLVTNDTLHALRALVGNSAAPARR
                     MRRTAFRSRRQVPPTAEGRWALVHAPGPSRSATEWSSALSHQLLARHGIVTRETAASE
                     AVVGGFSAVYQVLKAMEEAGRVRRGYFVAGLGGAQFAMPAALDLLRSLRDAPGEGTEG
                     VAVRTPQVVMVSATDPVCPYGAIVEWPSAGWADEIAHVTGAKGVTPKGRGPTRTVGSM
                     VILVDGHAAAYLRRGERELLLLGAATEPSRSTMTRETARALVRMSRSRDEGRQGLLIA
                     DIDGVAATTHPAARLFLDAGFVASAMGLQLRPGEKRVNM"
BASE COUNT          252 a          590 c          524 g          266 t
ORIGIN      
        1 gccttctcgg tggggatccc ctgctgcagc gagatctcct ggcgcacggt gccgctcgcc
       61 gcgccggtgg ccgggcccct cgtcaaattc ctcacgggca cgttgcgccg cacgagccgc
      121 gtctgcacga tttcccagag cgagttcagc ttgaactcgt cgtcggcgac gagcacgagc
      181 cgtgactcgt tcggatcgaa ctcaatcgac gctttcgatc ccttgaagtc gtatcgctgc
      241 gccacttcct tgcgcgcctg gttgagcgcg ttgtccactt cctgcaggtc cacgttggaa
      301 gtgatgtcga acgagcactg catcgccatg tgcgctcaca tgttaactcg cttctcaccg
      361 ggacgcagct gtaatcccat tgcggatgcg acgaagccgg cgtcgaggaa gagtcgcgcc
      421 gccggatgcg tcgtcgcggc cacgccgtcg atgtcagcga tcagcagacc ctgccgcccc
      481 tcgtcgcgcg agcgcgacat tcgcaccagc gctcgtgccg tttcgcgcgt catcgtcgat
      541 cgtgacggtt cggtcgcagc gccgagcagc agaagctcgc gctcgcctcg acgcaggtac
      601 gcggccgcgt ggccgtccac gaggatcacc atcgagccca ccgttcgtgt gggaccgcgg
      661 cctttcggtg tgaccccctt tgcgcctgtc acgtgcgcga tctcgtcggc ccatcccgcg
      721 gaaggccatt ccacgatcgc gccgtacggg cacaccgggt cggtggccga taccatcacg
      781 acctgtggcg tccgcactgc cacaccttcg gttccctcgc ctggcgcgtc gcgcagggag
      841 cgcagcagat cgagtgcggc cggcatcgca aactgcgccc ctccgagccc ggccacgaag
      901 tagccgcggc gcacgcgtcc ggcttcctcc atggctttca gcacttggta cacggccgaa
      961 aagcccccga cgaccgcctc ggatgccgcg gtctcgcgcg tgacgatgcc gtgacgagcg
     1021 aggagctgat gggacagggc cgaactccac tcggtggccg accgcgaagg gcccggcgca
     1081 tgtacgagcg cccaccgccc ctccgcagtg ggcggcacct ggcggcggga tcgaaacgcc
     1141 gtgcgtctca tccggcgcgc gggcgccgcc gagttcccga cgagcgcacg cagtgcgtgc
     1201 agcgtgtcgt tggtgacgag gcccttccac accagatccc agagcgcatc aaccgtctcg
     1261 ccgggaaacc ctccgccggt tccttcgtgg acggcgccga agaacgacgc accgtgcgat
     1321 cgaaggtagg tcaggatgtc gcccgctcgc ccttcgatgg tggcaggaga gccagacggt
     1381 cccagtcgtg caaagtggtc cgtcaggtac agcgcgatgc ggccgtcgcg cggctcgacg
     1441 ccggaccaca cgacttcgcc cgccgcgatg agcgtgtcca gcatcccggg ctcgtaatcg
     1501 gcgacgcgcg cgctcagcac gtcccgctcg agcaccgaga agggcagtgg cgcgccttgc
     1561 agttgctcga ccgcgtcgag cagcgcttcg agtcccctcc gcgcggatcc gatcccgtgc
     1621 cacgacacca gg
//
LOCUS       DBNGSH010000126        10938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2386141, whole genome shotgun sequence.
ACCESSION   DBNGSH010000126 DBNGSH010000000
VERSION     DBNGSH010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2386141"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC16CY_02865"
     CDS             complement(<1..815)
                     /locus_tag="AC16CY_02865"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3352749.1"
                     /translation="MGIEGAAKGKRIEVGAQPVTIGRDAGQTLVFADTQVSRFHARVS
                     LVNGEVVVEDLGSTNGTFVHTERITTPWTLRDGNVLRVGSQFLKYERRSRGDVKQAQD
                     LHRDLNKASNYVFSLLPAPITTGPVRADWRFLPSTQLGGDAFGYDWLDENTFVFYLLD
                     VSGHGAGSAMHSVTVLNVLRQRALPNVDFRNPGEVLSSLNTRFQMDGHNGMYFTMWYG
                     VFGARDRTLSYSSGGHHPAYLVPSDRRAAQPLGSPALMVGALPNSEFEVHQTTV"
     gene            complement(992..2098)
                     /locus_tag="AC16CY_02870"
     CDS             complement(992..2098)
                     /locus_tag="AC16CY_02870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3352750.1"
                     /translation="MTTHRETASTDDIVVNDLWPRLLLSPVIGAVIPNLAGFIDNGRY
                     SAGQLVAHYVYFTFVALAIWEGNRRLHNRARSRFAWFEQPWMRIGLLLGTIALFTVPA
                     TTALLWGWGIVSADPGMTPARLVLAVVVTVMSVVVIAHVYETLFMFRYWERDRLSRER
                     LERARLQAELDALQRDVDPHFLYNSLNSLSELIETRPQRALVFVEALAATYRYLVDTR
                     GCSLVPLADELAALRRQQVLADIRFGEAVRLDDSVEPGDARHWSLPPLVLQELFENAI
                     KHNAFDRQAPLDVRVERDGSDLLVSNPLVPRPAPARSTRMGLANLDERLRLAVGRSMR
                     WGTSGSRFVVRVPLARAAAVEHVERDRLGGQIAS"
     gene            2425..2598
                     /locus_tag="AC16CY_02875"
     CDS             2425..2598
                     /locus_tag="AC16CY_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352751.1"
                     /translation="MMRIAFGVAVLAALLGTYWVSPSAAWVALFATSTLALARHIRQR
                     DDEAEQALNPIRE"
     gene            2933..4360
                     /locus_tag="AC16CY_02880"
     CDS             2933..4360
                     /locus_tag="AC16CY_02880"
                     /inference="COORDINATES: protein motif:HMM:NF020390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HIR3352752.1"
                     /translation="MNQTLRALNRFGLGARRGERSRITDGRGWLRAQLEGGAPTVPAP
                     EGASPAAIGEAVRGFRTIGQGNDQERREARQRLVQLGTSEVLGALTTRVTTDRPFVER
                     LVAFWSNHLCVSTGAKILVAPFAGSYERDVIRPHVLGRFEDMVMASAKHPAMLVYLDN
                     FQSIGPASPGARVGGGRGNGQRRGLNENYARELLELHTLGVNGGYTQQDVQELARILT
                     GWTVNALGPGGRGAQRRPAQRLGRGRVSAEPVADDNDPIGFAFQPLLHEPGGKTVLRT
                     RYDEAGVAEGERAIRALCQHPSTATFVATKLVTHFVSDEPPAAAIERVAKAFRSSGGD
                     LKTVARALADEPEAWREDTRKFRTPQDWFVAVLRAFGARDVAANAPVLLRQLRQPFWS
                     PASPKGFGDAMQEWADPDSLLNRGELARTISRQPALRDLDPRALVDVVDVAPGNPLHN
                     LLADTSIPSPERVALAIAGPAFQWR"
     gene            4363..5526
                     /locus_tag="AC16CY_02885"
     CDS             4363..5526
                     /locus_tag="AC16CY_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019040.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3352753.1"
                     /translation="MNRREFVRTMCYGGIATFGLPSVSFAQVAQGSRLVFVLLRGGFD
                     GLAAIVPYGDPEYRSLRGAFAFNQSELTPLDDTFGLAPGLSPLRAFWDSNELAVVHAM
                     AIPHRTRSHFDGQAILETGLDRPVGSSDGWLNRLLQVMSGKRSGIAIAAGMPLSLTGS
                     YQVESWSPTQLGAVDDAFLERLAVLYRADAALSNRFEAALQQQDLVGEEPMARGRAAR
                     GGSTALMQAAARILRQEQGPNIAAMEFSGWDTHANQGMAGGALDRLLGQLAEGLVAFR
                     TEMGAAWRNTTVVVMTEFGRTARPNGTRGTDHGTAGAGFVLGPGVARAQVVSDWPGLG
                     ASALFEGRDLKPTLDTRAVLKAAVAGCFDLTPAQVERVFPDSSGVRPVAGLMP"
     gene            complement(5544..6356)
                     /locus_tag="AC16CY_02890"
                     /pseudo
     CDS             complement(5544..6356)
                     /locus_tag="AC16CY_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
     gene            complement(6353..6829)
                     /locus_tag="AC16CY_02895"
     CDS             complement(6353..6829)
                     /locus_tag="AC16CY_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3352754.1"
                     /translation="MGLVKAILLVEDSQDDIDLTMRAFRKNGMANRVAVVRDGVEALD
                     YLVLASGRDAAERPQLVLLDLKLPRLDGLQLLQRIRMNPATRFLPVVILTSSTEERDL
                     VNSYTLGANSYVRKPVDFEQFVQTLQQIGSYWLLMNQAPPMGPTSRVGSSSEEVKA"
     gene            complement(6817..8286)
                     /locus_tag="AC16CY_02900"
     CDS             complement(6817..8286)
                     /locus_tag="AC16CY_02900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3352755.1"
                     /translation="MSDGMLDVAEDIPLLFTGNPLPMWLCDPDSLSILAVNDRAVALY
                     GYSRGEFLRMRVGDLAGDPGRHCLRSGRIIDVEVASEPMEVSGRPAILMVIHDVTSRK
                     ETERALARSEAQRKALIESAPDPIITIDAAGRVLEFNPAAERAFGYRREAVIGALLVD
                     LVVPADPRERPHGGLAGWRPPILERKRLEMQAVRANGTGFPVEVAVTRLDDGAEQRCV
                     VHLRDISDQTRAEEGLHELNTELEERVRRRTVQLQAVVDELEAFSYSVSHDLRAPLRS
                     IDGFSQALAEDAGTALPEAARRHLDRIRAATQRMSHLIDDLLSLSTVSLARMTREHID
                     ITGLAGAILIELAGHSANRSVHVRIAPGMSASGDPRLVRVALENILTNAWKFTSRRED
                     ASIEVGQTLRDATQVFYVRDNGVGFDATYADKLFAPFQRLHDASEFPGTGIGLATVRR
                     IVQRHGGRVWADGQTARGACFYFTLEEAPRTESRKEWAS"
     gene            complement(8283..10076)
                     /locus_tag="AC16CY_02905"
     CDS             complement(8283..10076)
                     /locus_tag="AC16CY_02905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3352756.1"
                     /translation="MKDNQQRSRPGGLQSAHETSEPTSLREPEVTGRDSLRLLLIEDS
                     ADDADLIVRELSRGGYRVSPERVDTRDALACALGRQTWDVAIADFTMPGFSGTAALAL
                     VREYDAEMPVIFVSGTIGDDAAVMAMRSGAQDYLMKGNLKRLAPAVERELRDAVARRS
                     RRKAEAQLAHLAYHDALTDLPNRVLLNDRLRQAMLAAERNRESLALMVLDLDGFKAIN
                     DSLGHYAGDRMLQQVASRLRSLLRDVDTVARLGGDEFAFVLPRTDLEGAVLAARKILT
                     EVRRPHVLEGHPSSVGGSVGIVCFPEHGTSPELLLQKADIAMYAAKSGGLGYAAYDPD
                     RDRHKHGRLTLMVELREAIEGGQFTFDYQPIVDLKTGRPLCVEALARWDHPRRGRLPP
                     GEFIGFAEQSWLIEPLTMMLLDKALAEWGALDPSRTVPVAANLSPRTLHDPDFPDRLA
                     DLLRLCAAPPSALVLEVTENVLASDTARVTRCLSRLHAMGITIAIDDFGIGYSSLGYL
                     KRLPVDQLKIDRSFAGELSSGDDAIVRFTVDLAHNLGLTVVAEGVESAESWDRLRQLG
                     CDAAQGGFIASPASAAETRRWVAQRDAKDPP"
     gene            complement(10310..>10938)
                     /locus_tag="AC16CY_02910"
     CDS             complement(10310..>10938)
                     /locus_tag="AC16CY_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352757.1"
                     /translation="SAQIAHETRLDRKRVLRALLVVRRAMARSISEAVSLDNGGARAR
                     LALEDETRPARGLRPPRRAAALGLRASGTFAWAEVVPDVDTDGLARALRDRRGDGPFD
                     WPGAHRYAAVAYGGRLYRLAESARAPFGHVESFWAYLQRRLKAKGGIRRKRLGLYLAE
                     YAWRYNHRKLSPPAQLRELLKVIRRNQYGVGNTTYPVHREHDSKGGFH"
BASE COUNT         1876 a         3578 c         3676 g         1808 t
ORIGIN      
        1 accgtcgtct ggtggacctc gaattcactg ttgggcaggg cgccgaccat cagcgctggc
       61 gagcccaggg gctgcgccgc ccgccggtcg gacggaacga ggtaggcggg atggtggccg
      121 ccggagctgt acgacagcgt tcgatctcgc gcgccaaaca cgccatacca catcgtgaaa
      181 tacatcccgt tgtgaccgtc catctgaaac ctggtgttca ggctggagag cacctccccg
      241 ggattcctga aatcgacgtt gggcagggcg cgttgacgga ggacgttcaa tacggtcacc
      301 gagtgcatcg ccgagccggc gccgtggccc gagacgtcaa ggaggtagaa gacgaaggta
      361 ttctcgtcga gccagtcgta cccgaaggcg tcgcccccga gctgcgtcga cgggaggaag
      421 cgccagtccg cgcggaccgg ccccgtggtg atcggcgcgg gcagcaggga gaagacgtag
      481 ttgcttgctt tgttgagatc gcggtgcaga tcctgagcct gcttgacgtc gccacggctg
      541 cggcgctcgt acttgaggaa ttggctgccc acgcggagca cgttgccgtc tcgcaatgtc
      601 cacggcgtgg tgatgcgctc cgtgtgaacg aacgtcccgt tggtcgaccc cagatcctcg
      661 accacgacct cgccgttgac gagtgagacg cgggcgtgaa agcgcgaaac ctgagtgtcg
      721 gcgaagacga gcgtctgtcc cgcgtcgcgc ccaatcgtga cgggctgcgc accaacctcg
      781 atgcgttttc cctttgcagc gccttcgatt cccacaaggt aatgccccag gacctcgggt
      841 gctggggctg cttcgagggg ccgtccggga ctgatgaaga tcgtacggtc gagttcgttg
      901 tcttgagatg gtgacactga gctagggggt tatcgggcag catacgcgac cggcaagggc
      961 gcgcgcaacg ggccatatcg gccatggcgc ctcacgacgc gatctggccg ccgaggcgat
     1021 cccgttcgac gtgctccacc gccgcggcgc gcgccaacgg aactctcacg acgaagcgac
     1081 tgcccgatgt gccccaccgc atcgagcgcc cgaccgcaag ccgcaaacgc tcgtcgaggt
     1141 tcgcgagccc cattcgcgtg gaccgggcgg gcgccggccg cgggacgagc gggttgctga
     1201 ccaggaggtc gctgccatca cgctcgacgc gcacgtcgag cggcgcctgc ctgtcgaagg
     1261 cgttgtgctt gatggcgttc tcgaacaact cctgcaagac gaggggcggg aggctccagt
     1321 gccgggcgtc gccgggttcc accgagtcgt ccagccgtac ggcttcgcca aaccggatgt
     1381 cggcgagaac ctgctgccgg cgaagcgcgg cgagctcgtc tgccagcgga accagcgaac
     1441 atcctcgcgt gtcgacgagg tagcgatagg ttgccgcgag cgcctccacg aacaccagcg
     1501 ctcgctgcgg acgcgtctct atcaactccg acaggctgtt caggctgttg taaaggaagt
     1561 ggggatcgac gtcgcgctga agcgcgtcga gctcggcctg caggcgggcg cgctccagcc
     1621 gctccctgga cagccgatcc cgctcccagt agcgaaacat gaacagcgtc tcgtagacgt
     1681 gcgcgatgac gacgaccgac atcaccgtca ccacgacggc gaggacgaga cgagccggag
     1741 tcatgcccgg gtctgccgag acgattcccc acccccacag caatgccgtc gtcgccggaa
     1801 cggtgaagag cgcgatggtg ccgagcagca gaccaatgcg catccagggc tgttcgaacc
     1861 acgcgaacct ggatcgcgcc cggttgtgca gccggcggtt cccctcccag atagcaaggg
     1921 cgacgaacgt gaagtagacg tagtgcgcaa cgagctgacc ggccgaatac cgcccgttgt
     1981 cgatgaaacc cgcgagattc gggatgactg caccgatgac cggactgagg agcagccgtg
     2041 gccacagatc attgacgacg atgtcgtcgg tcgacgctgt ctcacgatgg gtagtcacac
     2101 tggctgaagt gtgagggact gggacgaaat ccgcaacgaa agcacagatt tcactgttcc
     2161 gcacgtgatc gatttcgaga cgttcgatcg tcgaagacac ttttcaacct aaggtctctg
     2221 cagtcaatag tttcacgtgt ctcgaacacc ttcgtgatgt tcgagtactg agcacaggcc
     2281 ccgacacgcc gaaggcgatt caccagcaca cgacggtcgg ctaacaaccg gtgtgtctgt
     2341 gacttgcgag gccgaatcga ggcagatgtc acatgtggac cgtagattgc tcactgtctc
     2401 aaacagagat tcttggggga cacgatgatg cgaatcgctt tcggcgtggc agtgctggcg
     2461 gcgttgctcg ggacgtactg ggtcagccca tcggcggctt gggtggcgct gtttgcaacc
     2521 tccaccctcg cgcttgcgcg gcatatccgc cagcgggacg acgaggccga acaggcactg
     2581 aacccgatcc gggagtaaca ggacaacagg ccgatccgcg cccgacgctt gccacgttgg
     2641 gcgcgaccac cttcaggtcc gacctcccct cgcgaagctg ctcctccctt cgcgtcggac
     2701 cgaaccagcc gggctcccgc tctcacccaa cccactcaat cagggctcca tcccagccgg
     2761 cgggaatttg ggtgcaagtc catgaagaag ttgcgtatcc tcggccgata acgcgagagt
     2821 gccccgggga gtcgaggcgg tggaccggca acgcacggcg gggtcgaccc gtcttacgtg
     2881 acgagcccgc tcagaacggg atcggatcgc attcgaagtc ggaggggtcg tcgtgaacca
     2941 gacgcttcgc gcactcaatc gattcgggct tggcgctcgc cgcggcgagc ggtcccggat
     3001 cacggatggc cgcggatggc tgcgcgccca gctcgaggga ggcgctccga cggttccggc
     3061 tcccgaggga gcttcaccgg ctgcgatcgg tgaggccgtt cgcggattca ggacgatcgg
     3121 ccagggaaac gatcaggagc gccgcgaagc caggcagcgg ttggttcaac ttggcacctc
     3181 ggaggttctc ggcgcgctaa ccactcgcgt caccacggac cggcccttcg tcgagcggct
     3241 cgtcgcattc tggtccaatc acctctgcgt atcgaccggg gcgaagatcc tcgtggcgcc
     3301 tttcgccggg agttacgaac gcgacgtcat ccgcccgcac gtgctcggac ggttcgagga
     3361 catggtcatg gcctcggcga agcacccggc gatgctcgtc tatctcgaca acttccagtc
     3421 gatcgggcct gcctcgcccg gcgcgcgcgt gggtggcggg aggggcaacg gacagcgccg
     3481 ggggttgaac gagaactacg cgcgcgagct gctcgagctt cacacgctcg gcgtcaatgg
     3541 cggttatacg cagcaggacg tgcaggagct tgcgcgcatc ctgaccggct ggacggtcaa
     3601 cgcgctcggc cctggagggc gcggcgcgca gagacggccc gcccagcggc tgggtcgcgg
     3661 gcgggtgtca gccgagcctg tggctgacga caacgatccg atcggttttg cgttccaacc
     3721 gctgctgcac gaaccgggcg gcaagaccgt gttgagaacc cggtacgacg aggccggggt
     3781 cgccgaagga gagcgggcga ttcgcgcgct ctgccagcat ccctccacgg ccacgttcgt
     3841 ggcgacgaag ctggtgacgc acttcgtcag cgacgagccg ccggccgccg cgatcgaacg
     3901 cgtcgccaag gcgttccggt cgagcggcgg agatctgaag acggtcgcgc gcgcgctcgc
     3961 cgacgagccc gaggcctggc gcgaggacac tcgaaagttc cgcacgccgc aggactggtt
     4021 cgtcgcggtc ctgagggcgt tcggtgctcg cgatgtggcg gccaacgcgc ccgttctgct
     4081 gcggcagctc cgtcagccgt tctggtcgcc tgcctcgccg aaagggttcg gtgacgccat
     4141 gcaggagtgg gccgacccgg attccctgct caatcgcgga gagctggcgc gcacgatctc
     4201 gcggcagccg gccctgcgcg atctcgatcc gcgcgcgctc gtcgacgtcg tggacgtcgc
     4261 gccgggcaac ccgctgcaca atcttttggc cgacacgtcg attccatcgc ccgagcgcgt
     4321 cgcgctcgcc atcgccggtc cggcattcca gtggaggtga cgatgaatcg ccgcgagttc
     4381 gtccggacca tgtgctacgg cgggatcgcc accttcggcc tcccctcggt cagcttcgcg
     4441 caggtcgcac aaggcagccg cctggtcttc gtgctgctcc gcggcggctt cgacggactg
     4501 gccgcgatcg tgccctatgg cgaccccgag taccgctcgc tccgcggggc gttcgcgttc
     4561 aaccagtccg aactgacccc gctcgacgac acgttcgggc tggcgcccgg cctgtccccg
     4621 ctgcgcgcgt tctgggacag caatgaactc gccgtggtgc acgcgatggc gatcccgcat
     4681 cgcacgcgca gccacttcga cggccaggcc attctcgaaa ccgggctcga tcgtccggtc
     4741 gggtcgtcgg acggttggtt gaaccgcctg ctgcaggtga tgtcgggcaa gcgatcgggc
     4801 atcgcgatcg cggccggcat gccgctttcg ctgaccggct cgtatcaggt cgagtcgtgg
     4861 tcgccgacgc agctcggcgc agtcgacgac gctttcctcg aacggctggc ggtcttgtac
     4921 cgcgcggacg cggcgctctc caaccgtttc gaggcggcgt tacagcagca ggatctggtt
     4981 ggtgaggagc cgatggcgag aggccgcgcg gcacgcggcg ggagcacggc gttgatgcag
     5041 gcggccgcgc gcatcctgcg tcaggagcag gggccgaaca tcgcggcgat ggagttcagc
     5101 gggtgggata cgcacgccaa tcagggcatg gcgggcggcg cgctcgatcg cctgctcgga
     5161 cagctcgccg aagggctcgt ggccttccgg acggagatgg gagcggcgtg gcgcaatacg
     5221 acggtcgtcg tgatgaccga attcggccgc acggcgcggc ccaacggcac tcgaggcaca
     5281 gatcacggaa cggctggcgc cgggttcgtg ctggggcccg gcgtcgcgcg agctcaggtc
     5341 gtctctgact ggcccgggtt gggggcgagc gcgctcttcg aagggcgcga tctgaagccg
     5401 acgctcgata cgcgtgccgt attgaaggcg gccgtggcag gctgcttcga cctgaccccc
     5461 gcacaagtcg agcgcgtgtt cccggactcg tccggcgtgc ggcccgtggc gggactcatg
     5521 ccgtaggaga ggagcacatc gcgcgcggtg ccgaaacgaa atcgagagag cagggctgag
     5581 agcttctcgg cctgtttcgc aacgccgaca tagtgacgaa aggcgtgccg ccacggcatc
     5641 ggagggcgtg gttgacccgt gtccagctcc cacgggtgca ggtagaacat cacagggcgc
     5701 ccttcggcgg agttgacgcg ttcgacgccc caccgcgtgt acgcgaacgg cgcaaggcga
     5761 aagtagccgc cgccgccaat cggcatgttc catccgaaga cgcgcgccgt gccgatggga
     5821 aactccacga gcgtcgcgga attctgacgc cagatctcat acggaaagcg gggcgcggtg
     5881 ggctgcccgt agcggtcgtg cacgatcggg tggctgctcg agtcgtacag aaaaccttct
     5941 tccaacagga tctgatacgc ccagcgctgg gcgcgcccga tcgagaagtt cggcgcgcga
     6001 tatccgacaa cggccgtccc caccacgtcc tcgatccgct gcttggcgcg ccgaagatcg
     6061 gcgcggaact cggacgggct ctgtcgatgg acgctctcgt ggcgatcgcc atgggaggcg
     6121 acctcgtgtc cttcggccgc aatccgccgg atcgactgcg ggtgcttcgc tgccacttct
     6181 ccgagaacga agaaggtcgc cttcgcggag tggatgctga acaacgcgag cagccgatcg
     6241 acgttccgtt cgacgcggct ctcgaggaag ttcccggaga cggtcccggc cccgcggcga
     6301 aagatttcag cgtggtagtg ctcctcgacg tccacactga gtgcgttcgt gatcatgcct
     6361 tgacctcttc ggacgatgaa ccgactcgac tggtggggcc catcggcggc gcctggttca
     6421 tcagcagcca gtacgagccg atctgctgca gcgtctgcac gaattgctcg aagtcgaccg
     6481 gcttgcggac gtagctgttg gcgcccagcg tgtagctgtt gacgaggtcc cgttcctccg
     6541 tcgacgatgt caggatcacg accgggagaa acctcgtcgc cggattcatg cggatgcgct
     6601 gcaagagttg cagcccatcg agccgcggca gcttcagatc caggagcacg agctggggcc
     6661 tctcggcggc gtcgcggccg ctcgccaaaa ccaggtaatc cagcgcttcg acgccgtcgc
     6721 gaacgaccgc gaccctgttg gccatgccgt tcttgcgaaa cgcccgcatc gtcaggtcga
     6781 tgtcgtcctg gctgtcttcg acgaggagga tcgctttcac gaggcccact ccttcctcga
     6841 ctccgtccgc ggcgcttcct cgagcgtgaa atagaagcag gcgccacgcg ccgtctgtcc
     6901 atcggcccac acgcgcccgc cgtgccgctg cacgatccgc cgcaccgtcg ccaggccgat
     6961 gccggtgcct ggaaactccg acgcgtcgtg cagccgctgg aagggggcga acagcttgtc
     7021 ggcgtacgtg gcatcgaagc cgaccccgtt gtcacggacg tagaacacct gagtcgcatc
     7081 tcgcaacgtc tgcccgacct cgatggatgc atcctcccgg cggctcgtga acttccaggc
     7141 gttggtgagg atgttttcga gcgccacgcg gacgagccga ggatcgccgg aggctgacat
     7201 gccaggcgcg atgcggacgt gaaccgatcg attcgccgaa tgcccggcca gttcgatcag
     7261 gatggcgccc gcgagaccag tgatgtcgat gtgctcgcgc gtcatcctgg cgagtgacac
     7321 cgtcgacagg ctcaggagat cgtcgatgag atgcgacatg cgctgcgtcg cggcgcgaat
     7381 gcggtcgagg tgccgtctgg cggcctcggg cagcgccgta cccgcgtcct cggcgagcgc
     7441 ctggctgaag ccgtcgatgc tccgaagcgg ggcgcgcagg tcgtgtgaga ccgaatagct
     7501 gaacgcctcc agttcgtcca ccacggcttg aagctgcacg gtgcgccgcc ggacacgttc
     7561 ctcgagctcg gtgttcagct cgtgcagccc ctcttcggcg cgtgtctggt cggagatgtc
     7621 gcgaaggtgg acgacgcatc gttgctcggc gccgtcatcg agccgggtca ccgcaacctc
     7681 gacgggaaac ccggtgccat tcgcgcgcac agcctgcatc tccagccgtt tgcgctccag
     7741 gatcggcggg cgccagcctg ccaggccgcc gtggggcctc tcgcgcgggt ccgccgggac
     7801 aacgaggtcc acgagcagtg ctccgatgac ggcctctcga cggtagccaa acgcgcgctc
     7861 ggcggccgga ttgaactcga gcacgcgccc ggcggcgtcg atcgtgatga tcgggtctgg
     7921 cgccgactcg atgagcgcct ttcgctgggc ctcgctccgg gccaacgcgc gctcggtttc
     7981 cttccgggac gtgacgtcgt ggatcaccat cagaatcgcc ggccggccgg agacctccat
     8041 cggctccgac gccacttcga cgtcgatgat gcggccgctc cgcaggcaat gacgtccggg
     8101 gtcgcctgcc aggtctccga cgcgcattcg caggaactcg ccgcgtgagt atccgtagag
     8161 cgcgacggcc cggtcgttga cggcgaggat cgagagcgag tcgggatcgc acagccacat
     8221 gggtaacggg ttgcccgtaa acagcaacgg gatgtcttcg gcaacgtcga gcattccgtc
     8281 gctcatggcg gatccttcgc gtcccgctgg gcgacccacc ggcgagtctc cgccgcggac
     8341 gctggcgatg cgatgaagcc cccctgggcc gcgtcgcacc cgagctgacg aagccggtcc
     8401 caggactccg cggattcgac cccttcggcg acgaccgtca agccgaggtt gtgtgccaga
     8461 tcgacggtga atcgaacgat cgcatcgtct cccgacgaca gctcgccggc gaacgagcga
     8521 tcgatcttca gttgatcgac cggcaggcgc ttgaggtagc ccagcgagga atagccaatt
     8581 ccgaagtcat cgatcgcgat cgtgataccc atcgcatgga gccgcgagag acacctggtg
     8641 acgcgggccg tatcggaggc gaggacgttc tcggtgacct ccagcacgag cgctgacgga
     8701 ggggcggcac agaggcgcag cagatcggcg agtcgatcgg gaaaatccgg atcgtgcagc
     8761 gtccgcggcg acagattcgc cgcaaccggc acggtccgcg acggatcgag cgccccccat
     8821 tcggcgagcg ctttgtcgag gagcatcatc gtcagcggct cgatcagcca cgactgctcc
     8881 gcgaacccga tgaactcgcc gggcggcagc cggccgcgcc ggggatggtc ccagcgggcg
     8941 agtgcctcga cgcacaaggg ccgccccgtt ttcaggtcga caatcggctg gtagtcgaac
     9001 gtgaactgcc cgccttcaat cgcttccctc agctccacca tcagcgtgag ccgtccgtgc
     9061 ttgtgccgat cacggtcggg gtcatacgcg gcgtacccaa gcccgccgct cttggctgcg
     9121 tacatcgcga tgtcggcctt ctgcaggagc agctccggac tcgtgccgtg ctcggggaaa
     9181 cagacaatcc cgacgctgcc gccaacgctc gacggatgac cctcgaggac gtgcggtcgg
     9241 cggacctcgg tcaggatctt gcgtgccgcg agcacggcgc cctcgaggtc ggtccgcgga
     9301 agcacgaatg cgaactcgtc gccgccgagc cgcgcgaccg tgtccacgtc acgaagcagc
     9361 gagcggagcc tcgacgcgac ctgctgaagc atgcggtcgc cggcgtaatg gccaagcgaa
     9421 tcgttgatcg ccttgaaccc gtcgaggtcg aggaccatca gggccagcga ctcgcgattg
     9481 cgctcggccg cgagcatcgc ctgtcgaagc cgatcgttca ggagaacccg gttcggcaga
     9541 tcggtcaggg cgtcgtgata ggccaggtgt gcgagctgag cctctgcctt cctgcgagag
     9601 cggcgagcaa cggcatcgcg cagctcgcgt tcgacggcgg gcgcgagccg cttcaggttg
     9661 cccttcatga ggtaatcctg ggcccccgat cgcatcgcca tcacggcggc atcgtccccg
     9721 atcgtgcccg acacgaaaat gacgggcatc tcggcgtcgt attcgcgcac gagtgccagc
     9781 gcggctgtcc cgctgaagcc cggcatcgtg aaatcggcta tcgccacgtc ccaggtctgc
     9841 cgtcccagcg cacaggcgag cgcgtcgcgc gtatcgactc gttcgggcga cacgcgatac
     9901 ccgcctcgcg acagctcgcg cacgatgaga tcggcatcgt cagccgagtc ttcaatcaga
     9961 agcagccgca gggaatccct tcccgtgacc tcaggctcac gaagggaagt cggctccgac
    10021 gtctcgtgag cagactgaag gccgccgggc cggctacgtt gctggttgtc cttcatcttg
    10081 gcggtccgtc ggtccgctga ggccacgata cgaaaagccg caacacgctg gtagcgggta
    10141 taaacctgag tgcggcggcg gcgggatccg gaggattcgc gagcggctct tgtagagacc
    10201 gcgagtgaaa atgtctgaat gggagtgaaa tctgtaagtc gaaggtagat ttttgaaacg
    10261 gcgagtgtaa ttcctgcttc acgccgtagg gtctatacct gatgcctcct caatgaaaac
    10321 cccctttact gtcgtgctcg cgatgcacag ggtaagtcgt gttgccgaca ccgtactgat
    10381 tgcgacgaat caccttcagg agctcgcgaa gctgcgcggg cggtgacaac ttccggtggt
    10441 tgtagcgcca ggcatactcg gcgaggtaga gtcccagccg tttccttcga atgcctccct
    10501 ttgccttcag ccggcgctgc aggtacgccc agaaactttc gacgtgcccg aacggggcgc
    10561 gagctgattc ggcgagccga tagagtcgtc cgccatacgc cacggccgcg tatcggtgag
    10621 cgcctggcca gtcgaacggg ccgtcgcccc ttcgatcgcg cagcgcgcgc gcgagcccgt
    10681 cggtgtcgac atcgggtacg acctcggccc acgcaaacgt gccggacgca cgaagtccga
    10741 gggcggcagc gcggcgcggt ggcctcaggc cccgggcggg acgcgtctcg tcctcgagcg
    10801 cgagacgggc ccgcgcgccg ccattgtcga gactgacggc ctcggaaatc gaacgagcca
    10861 tcgcccgtcg gaccaccagg agcgccctca gcacgcgctt ccggtcgagg cgagtctcgt
    10921 gcgcgatctg tgcgctcg
//
LOCUS       DBNGSH010000127         3729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4084791, whole genome shotgun sequence.
ACCESSION   DBNGSH010000127 DBNGSH010000000
VERSION     DBNGSH010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4084791"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2735
                     /locus_tag="AC16CY_02915"
     CDS             <1..2735
                     /locus_tag="AC16CY_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR3352758.1"
                     /translation="EPVPIGGDVVYAFGPEDIEIAPSRLATQSTFVEFDGRTAYGEDS
                     RFPFHVTSADWQESDRVFAGLLTAFGVRTRAIPIEGHGTFDGIMQGSVRRPRIEGDFS
                     GDRMRAWDVLWGAVKGHAVIENAYVDASNVEITSGDSLIATTGRFSIGYPRRDGGEEI
                     NARIQISGRPVADLRNAFRLYEYDLDGILSGDFDVRGKYLTPTGSGTMEITNGTAYGE
                     PFESARAAVQLEGEGVRLENLQAMKGGARGTGAAYVGFDGTYNFNFDAQGIPVESFAA
                     AKRLPLPISGLLDVTAGGSATFASPRYDVRATMRDLFVADEGVGQVYGEFSVANNLMT
                     VRRLDVASPRLGVTGSGRIALNPELDTEFSLLVSDASLDPYIRLFQPRVSPYTTAVAS
                     GSIRVVGSLADIDNLLVDATVDRLDVTLFDYALRNARPIRMALDRHSIRVNDMRIEGL
                     DTQLDVTGQVNLHDRRIAIRTTGDANLAVLQGIFPNISSRGMATLSATLEGPLENPDA
                     TGALELANGRIRHFALPHALENINGPLRFDARGVSLDGLTGRLGGGDVSFKGRVDKDG
                     YLPGRLDLTMAGTDMRLRFTEGGQALVDAQLALQGTIEDALLSGRVTVKEALYRRTLS
                     TGVGFFNFGGDDDIPQPEGVTPTLPVRYDVRIEAPSSIRVRNNVFPSLVASADLRLRG
                     TFARPLLFGRFEVDRGEAQFEGKRYVLTRGTIDFNNPTRIEPFFDIEAETRVRVPGET
                     YRVTVRAAGPQDRLDPVLTSDPPLPEVEVLALLFGNIAPGGNAELNQYSTVITPQQQL
                     MRAQLAQQLTGGISSEVGRAVAGAVGVDTFQITPLLGDPNAQTARLEPGARVTIIKNL
                     SPRVALTYQRSLSSSSRDQIILLEYDPTDRYSWILSRNEDGTFALDLRVRRTFR"
     gene            2736..>3729
                     /locus_tag="AC16CY_02920"
     CDS             2736..>3729
                     /locus_tag="AC16CY_02920"
                     /inference="COORDINATES: protein motif:HMM:NF018899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HIR3352759.1"
                     /translation="MRAFVRVLLGLGYAVWILTDAAPARAQVGERVVEIVIEQEGQRV
                     TEPTITRLIQTRVGEPLSIADVRETTDHLYSLRRFDDVQTSSEPVPNGIRLRYTLFPL
                     HPIDRIEFRGTLGLSQGSLRTLVVDRFGDSPRSGQLVEVAEALRAEYRRKGYDRASIT
                     PRVEESHNPDRATLVIDVQSGPRQTITDVRFTHVDPERSVKIEELPDVKRGQPFDEDA
                     IESELRAWEERMRARDYYEATASHGSITGDGIFVTVSLALGPRVVLGFAGDPLPKSEL
                     EQLVPIRAEGSADEDLLEDSRRAIEAYLSARGYRDAAAPVSREERGGDLIITFDV"
BASE COUNT          621 a         1285 c         1229 g          594 t
ORIGIN      
        1 tcgagccagt gccgataggc ggcgacgtcg tctatgcctt cggccccgag gacatcgaga
       61 tcgcgcccag ccggctggcg acccaatcga cgttcgtcga attcgacggg cgcaccgcct
      121 acggtgagga ctcgcgcttt ccgtttcacg tgacgagcgc cgactggcag gaaagcgatc
      181 gcgtgtttgc cggcctgctg acggcgttcg gcgttcgcac gcgcgccatt ccgatagagg
      241 gccacggcac cttcgacggg atcatgcagg ggagcgtccg gcgcccgcgc atcgaaggcg
      301 acttctccgg cgaccggatg agggcctggg acgtcctctg gggcgcggtg aagggccatg
      361 ccgtcatcga gaacgcctac gtcgatgcct cgaacgtcga gatcacctcg ggcgactcgc
      421 tcatcgccac aacgggccgg ttctcgatcg ggtacccgcg tcgcgacggc ggcgaggaga
      481 tcaacgcccg catccagatc tcgggccgcc ccgtcgccga tctgcgcaac gccttcaggc
      541 tgtacgaata cgacctggac ggcatcctga gcggcgactt cgacgtccgc ggcaagtacc
      601 tgacgccgac cggctcgggg acgatggaga ttaccaacgg cacggcctat ggcgagccgt
      661 tcgaatcggc gagggccgca gtgcagctcg agggcgaggg cgtgcgcctg gagaatctcc
      721 aggccatgaa gggaggcgcg cgcggaaccg gcgcggccta cgtcggcttt gacggcacct
      781 acaacttcaa cttcgacgcg caggggattc cggtggagtc gttcgcggcc gcgaagcgct
      841 tgccgctgcc catctcgggt ctgctcgacg tgacagccgg cggcagcgcc acgttcgcgt
      901 cgccgcggta cgacgtgcga gccaccatgc gcgacctgtt cgtcgccgac gaaggcgtcg
      961 gccaggtcta cggagaattc agcgtcgcca acaacctgat gacggtgcgt cggctcgatg
     1021 tcgcgtcgcc tcggttgggc gtcaccggct ccggccgcat tgcgctgaac ccggagctcg
     1081 acacggagtt ctcgctcctc gtctcggacg catccctcga cccctacatc cgcctcttcc
     1141 agccccgcgt gtccccgtat acgacggccg tcgccagcgg ctcgatccgc gtggtcggct
     1201 cgctggccga catcgacaac ctgctcgtgg acgcgacggt ggaccggctc gacgtcacgc
     1261 tgttcgacta cgcccttcgc aatgcgcggc ccatccggat ggcgctcgat cggcattcga
     1321 tccgcgtcaa cgacatgcgc atcgagggcc tggacacgca gctcgacgtc accggccagg
     1381 tcaacctgca cgatcggcgc atcgcgatcc ggacgaccgg cgacgccaac ctggccgtgc
     1441 tgcagggcat cttcccgaac atcagcagcc gcggcatggc gacgctgtcg gccacgctcg
     1501 aaggtcctct cgagaacccc gatgccaccg gcgcgctcga gctcgccaac ggccgcatcc
     1561 gccacttcgc gctgccgcac gcgctcgaga acatcaatgg accgctgcga ttcgacgcgc
     1621 ggggcgtatc gctcgacggg ctgaccgggc gtctcggcgg cggcgacgtg tccttcaaag
     1681 gccgggtgga caaggacggg tacttgccag gccggctcga cctgacgatg gccggcacgg
     1741 acatgcgcct gcggttcacc gagggcggac aagcgctggt agacgcacag ctcgcgcttc
     1801 aaggcaccat tgaagacgcg ctgctgtctg gacgcgtgac ggtcaaggag gcgctctaca
     1861 gacgcacgct ctcgaccgga gtcggtttct tcaatttcgg cggcgacgac gacataccgc
     1921 agcctgaagg agtgacgcca acgctccctg ttcgctacga cgtgaggatc gaggcgccgt
     1981 cgtcgatccg cgtccgcaac aacgtctttc cgtcgctcgt cgccagcgcc gacctccggc
     2041 tgcggggcac cttcgccagg ccgctgctct tcgggcgctt cgaggtcgat cgtggcgagg
     2101 cacagttcga gggcaagcgc tacgtcctga cgcgcggcac catcgacttc aacaacccga
     2161 cgaggatcga gcccttcttc gacatcgaag cggagacgcg ggtccgcgtt cccggcgaga
     2221 cgtatcgcgt caccgtgcgc gcggcagggc cccaggaccg gctcgacccg gtgctgacct
     2281 ccgaccctcc tctgccggag gtcgaggtgc tggcgttgct gttcggcaac atcgcgccgg
     2341 gaggcaatgc cgagctgaat cagtacagca cggtcattac accgcagcag cagctcatgc
     2401 gcgcccagct cgcgcagcag ctcacgggcg gcatctcctc cgaggttgga cgggcggtcg
     2461 ccggcgccgt cggcgtggac accttccaga tcacgccgct gctcggcgat ccgaacgcgc
     2521 agacggcccg cctcgagccc ggcgctcggg tcaccatcat caagaacctc tcccctcgcg
     2581 tggcgctgac ctaccagcga agcctgtcat cgtcttcgcg ggatcagatc atcctgctcg
     2641 agtacgatcc gactgaccgc tactcctgga tcctgtcccg caacgaggac ggcaccttcg
     2701 cgctggatct cagggtgcga cggacgtttc gctgaatgcg agccttcgtt cgggtgctcc
     2761 tcggtctcgg ctacgccgtc tggatcctga ctgacgccgc accggcccgc gcccaggtgg
     2821 gcgaacgggt agtcgagatc gtgatcgagc aggaggggca gcgggtcacc gagcccacga
     2881 tcacacgatt gattcagacg cgcgtgggcg agcccctgtc gattgccgac gtcagggaga
     2941 ctaccgacca cctctacagc ctgcgccgct tcgacgatgt gcagacgtcg agcgagcccg
     3001 tcccgaacgg catacggctt cggtacacgc tgtttcccct ccacccgatt gaccgcatcg
     3061 agttccgggg caccctcggg ctgtcgcagg ggtccctccg tacgctggtg gtggaccgtt
     3121 tcggcgactc gccgcgatcg gggcagctcg tcgaggtcgc cgaggcgctc cgtgcggagt
     3181 accggcggaa aggctacgac cgcgccagca tcacgccacg cgtggaggag agccacaatc
     3241 ccgatcgcgc gacgctcgtc atcgacgtgc agtcaggccc gcgccagacg attaccgacg
     3301 tgcgcttcac ccacgtcgac cccgagagga gcgtaaagat cgaggaactg cctgacgtga
     3361 agcgaggcca gccgttcgac gaggacgcga tcgagagcga gctgcgcgcc tgggaagagc
     3421 ggatgcgcgc ccgggactac tacgaggcga ctgccagcca cgggtcgatt acgggcgacg
     3481 gcatattcgt gacggtgagc ctcgcgctcg ggcccagagt cgttctgggc ttcgcggggg
     3541 atccgctgcc caagtccgag ctcgagcagc tggttcccat ccgcgccgag ggatccgcag
     3601 acgaggacct gctcgaagat tcgaggcgcg ctattgaagc gtacttgtcg gctcgtgggt
     3661 accgcgacgc cgccgcgccg gtcagcaggg aagagcgggg cggagatctc atcatcacgt
     3721 tcgacgtcg
//
LOCUS       DBNGSH010000128         2180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3065905, whole genome shotgun sequence.
ACCESSION   DBNGSH010000128 DBNGSH010000000
VERSION     DBNGSH010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3065905"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="AC16CY_02925"
     CDS             <1..653
                     /locus_tag="AC16CY_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352760.1"
                     /translation="TVGVLVRGERDAGPIEHPPLMPEVVARAAAFSIERFIESARALF
                     PRYARLGAALMIVYLVSGGAYLGLLARWAGGGLGVSALLTAAFVAWITLVNLFYLLTQ
                     IVVAADDCSVTTAAGRVSLFLRREARAVSGVFLVVLALVVLATGASVLATGALLLVGS
                     VPFFGLAVLPLQLLAWVLRALVFQFIGLASVGAYVRLYRTETGETTVGRLSTAEAS"
     gene            650..1858
                     /locus_tag="AC16CY_02930"
     CDS             650..1858
                     /locus_tag="AC16CY_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR3352761.1"
                     /translation="MTDYARFLSPAGRQLTESAIRRMGTVVARSGDLVSFAPGYPDAT
                     LFPWAELRDIASSVLDGHDDSALQYGPTRGYAPLLRTALGLLATRGIEASLEELIITS
                     GSQQGIDLIARVLVCPGDVVLVELPTFTGAIAAFKNVQATLVGVQQEADGISLEHLDE
                     AWRRESGAGKTIKLLYLIPNFQNPTGSLLSLRKRARLLEWAERRDVLLVEDDPYGQLY
                     FADVATEADTRPMRADDRNGRVLYLSTVSKILAPGFRVGWMLAPSMLIERFDTAKQST
                     DLTSGILDQRIVNEALRRGVADRLAPVLRERYRLKRDVMEQALRANLGPRLTWTTPRG
                     GFFVWATLQEGWTDSSLLERAIERGVVFVAGSAFHVDGSGRDTIRLSFSAPSPERIEE
                     GVRRLAAAFV"
     gene            complement(1879..>2180)
                     /locus_tag="AC16CY_02935"
     CDS             complement(1879..>2180)
                     /locus_tag="AC16CY_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352762.1"
                     /translation="RNLGLSTLNALTGEAIATLATPPDPAGVPLVDQVLRPFRVAMVA
                     LTRTNRAIGLRPTAMMFRERPLAPLLPLPGRALSREPFPKPDSKTAGSGLPPVKP"
BASE COUNT          307 a          702 c          754 g          417 t
ORIGIN      
        1 ggaccgttgg cgttcttgtg cgcggagagc gagacgccgg cccgatcgag caccctccgc
       61 tcatgccgga agtcgtcgcg cgcgccgcgg cgttttcgat cgagagattc atcgagtcgg
      121 cccgggcgtt gttccctcgc tacgcgcgtc tcggcgcggc gctcatgatc gtctacctcg
      181 tgtcgggggg cgcatatctg ggtctcctcg ctcgttgggc cgggggagga ctgggagttt
      241 ccgccctgct caccgctgcc ttcgtggcct ggatcacgct cgtcaacctg ttttacctcc
      301 tgacgcagat cgtggtcgcc gccgacgatt gctcggtgac gacggcagcc gggcgcgtgt
      361 cgctgtttct gcgccgggaa gcacgagccg tctcaggtgt gtttctcgtg gtgctggcgc
      421 tggtggttct ggcgacgggg gcgtccgtcc ttgccaccgg cgcgctattg ctggtcgggt
      481 ccgtgccgtt cttcggtctt gccgtccttc ctctacagct gctggcgtgg gtacttcgcg
      541 ccctggtgtt tcagttcatc ggtctcgcct ccgtcggcgc ctatgttcgg ctttatcgaa
      601 ccgagacggg cgagacgacc gtgggccgtc tgtctactgc cgaagcttca tgaccgacta
      661 cgccagattc ctttcgcccg cggggcgaca gctgaccgag tctgccatca ggcgcatggg
      721 caccgtggtc gcgcggtcag gtgacctcgt gtcgttcgcg cctgggtacc cggacgccac
      781 gctgttccca tgggcggagc tgcgcgacat cgcgtcatcg gtcctcgatg gacacgacga
      841 cagcgcgctc cagtatgggc cgacgcgcgg ctacgcgccc ctgctgagga cggcgctggg
      901 cctgctcgcc acgcggggaa tcgaggcgtc gctcgaggag ctgatcatca cgagcggatc
      961 tcagcaggga atcgacttga tcgcccgcgt gctggtctgt ccgggggatg tcgtcctggt
     1021 cgagctgccg acgttcaccg gcgccattgc cgcgttcaag aacgtgcagg ccacgctcgt
     1081 gggcgtgcag caggaggcgg acggcatcag cctcgagcac ctcgacgaag cctggcggcg
     1141 cgagtcgggc gcggggaaga cgatcaagct cctttatctc attccaaact ttcagaaccc
     1201 gaccggatcg ctgctctcgc tccggaagcg ggcgcgcctg ctcgagtggg cggagcgccg
     1261 cgacgtcctg ctggtcgagg acgacccgta cgggcagctc tacttcgccg atgtcgcgac
     1321 cgaggccgac acgagaccga tgcgcgccga tgaccggaac ggccgcgtcc tgtacctgag
     1381 caccgtctcg aagatcctgg cgccgggttt ccgggtcggc tggatgctgg cgccctcgat
     1441 gctcatcgag cggttcgaca cggccaagca gtctacggat ctgacttccg gcattctcga
     1501 ccagcggatc gtgaacgagg cccttcgacg aggcgtcgcg gatcggctcg cccccgtgtt
     1561 gcgcgagcgc tatcgtctca agcgggatgt gatggagcag gcgctccgcg ccaatctcgg
     1621 accccgtctc acgtggacga cgcctcgcgg cggcttcttc gtctgggcga cgctgcagga
     1681 aggatggacg gactcatcgc tgctcgaacg cgcgatcgag cgcggcgtcg tgttcgtagc
     1741 tggcagtgca ttccatgtgg atggcagcgg ccgcgacacg atacggttgt cgttctccgc
     1801 accgtcgccc gagcgaatcg aagaaggcgt ccggcggctt gcggcggcct tcgtctgaca
     1861 ctccagtccg tcgattcgtc agggtttcac cggcggcagt ccggagccgg cggtcttcga
     1921 atcgggtttg gggaaaggct ctcggctcaa ggcacgtccc ggcagaggca gtagaggcgc
     1981 gagagggcgc tcgcgaaaca tcattgccgt ggggcgtagg ccgatcgcgc ggttcgttcg
     2041 cgtcagcgcg accatggcga cgcgaaacgg acggagcacc tgatcgacca ggggcacgcc
     2101 ggcagggtca ggtggcgtcg ccagcgtggc aatcgcctca ccggtgagcg cgttgagtgt
     2161 cgatagtccg aggttcctcc
//
LOCUS       DBNGSH010000129         1896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_179241, whole genome shotgun sequence.
ACCESSION   DBNGSH010000129 DBNGSH010000000
VERSION     DBNGSH010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_179241"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="AC16CY_02940"
     CDS             complement(<1..881)
                     /locus_tag="AC16CY_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HIR3352763.1"
                     /translation="MVGLRLASGIVIVAAAGAALETAGRQASSSPASVTSAQLRQIAY
                     LKGSNTEAGDHFGCGGVLDGHAGYGAAISGDGNTVAIGAPHESGGSSGVNGNQNDNSM
                     YGSGAVYVFVRSGSGWAQQAYVKPSNPQMSAEFGHAVALSADGNTLAVSAYWEASKAT
                     GINGDQKDESIPQAGAVYVFTRTGTRWAQQAYVKASNTGEAGTADSFGDGDQFGVSLA
                     LSDDGATLAVGAISEDSRASGINGNQADNTATSAGAVYLFSRTGSTWAQQAYIKAANP
                     DAGDLFGYSVSLNADGNT"
     gene            complement(918..1340)
                     /locus_tag="AC16CY_02945"
     CDS             complement(918..1340)
                     /locus_tag="AC16CY_02945"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3352764.1"
                     /translation="MMKGLLLSCVLAAGVLGLASQARAHHGDADRYVQEVVDITGTVV
                     ELKMVNPHAHIVFDVTDDKGKTVRWQAELGGPQQLTRQFGWTPNTVKKGMKLTMTGRR
                     LKSGSPYLNLTERARIVVTDTGKEIFRTENFGEPPPKS"
     gene            1646..>1896
                     /locus_tag="AC16CY_02950"
     CDS             1646..>1896
                     /locus_tag="AC16CY_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352765.1"
                     /translation="MRVAGKGIACAVAAVMWLSCLVLVSGQAAPTDAAPTAEQVFKNV
                     QVLKGIPVNEFMGTMGVFSAALGMSCEDCHGAGDADWGV"
BASE COUNT          291 a          590 c          655 g          360 t
ORIGIN      
        1 gtgtttccat cagcgttgag cgagacgctg tagccgaaca ggtcgccggc gtccgggttg
       61 gcggccttga tgtaggcctg ctgcgcccag gtgctgccgg tccgcgagaa gagatagacg
      121 gcgccggcgg acgtggccgt attgtcagcc tggttgccgt tgatgccgga tgcgcggctg
      181 tcttcggaga tcgctccgac cgcgagcgtc gcgccgtcgt cgctcagcgc cagcgacacg
      241 ccgaactgat cgccgtcgcc gaagctgtcg gccgttcccg cttcgccggt gttcgacgcc
      301 ttgacgtagg cctgctgagc ccacctcgtt cccgtgcgcg tgaacacgta cacggcgccg
      361 gcctgcggga tcgactcgtc tttctgatcg ccgttgattc ccgtcgcctt gctcgcctcc
      421 caatatgcag agaccgcgag cgtgttgcca tcggcgctga gcgcgacggc gtggccgaac
      481 tccgcgctca tctgcgggtt ggacggcttg acgtaggctt gttgtgccca cccgctgccg
      541 ctgcggacga acacgtagac ggcgcccgac ccgtacatcg agttgtcgtt ttggttgccg
      601 ttgacgccgc tcgagccgcc gctctcgtga ggtgccccga tcgccaccgt gttgccgtcg
      661 ccgctgatcg ccgcgccgta gccggcgtgt ccgtcgagca cgccgccgca cccgaaatga
      721 tcgccggctt ccgtgttcga gcccttgaga tatgcgatct gtctgagctg cgcagacgtc
      781 acggacgccg gcgaggacga cgcctgtcgg ccggcggttt caagcgccgc gccggcggcc
      841 gccacgatca cgatccctga agccagacgc aatccgacca ttgggcctcc acagaacgac
      901 gtgctcaggt gctcaggcta ggattttggt ggtggttcgc cgaagttctc ggtgcggaag
      961 atttccttgc cggtgtcggt cacgacgatg cgcgcgcgtt cggtcagatt gaggtacgga
     1021 gagccgctct tcaaccgtct gcccgtcatc gtgagcttca tgcccttctt caccgtgttc
     1081 ggcgtccatc cgaactgcct cgtgagctgc tgtgggccac ccagctcggc ctgccaccgg
     1141 acggtcttcc ccttgtcgtc ggtgacgtcg aacacgatgt gcgcgtgagg attgaccatc
     1201 ttcagctcga cgacggtgcc ggtgatgtcg acgacttcct ggacgtacct gtcggcgtcg
     1261 ccgtgatgcg cccgcgcctg cgacgcgaga cccaacacac cggccgccag aacgcacgac
     1321 aacagcaggc ctttcatcat tcgaaccctc ccagggctac ttcggaaatc tctcactatc
     1381 acgcaggtct gtcaaatccg ccttcgctgc ttcgcggctt cggcgagaca ggtccacctt
     1441 cgcgctgcgc gctacggcgg acaggtgcct ccgatgtcgg gtgatgtgaa aaacatcacg
     1501 ttgccgggcc aggggaaggg aaggataatc gggcggcaaa ggtacgaagg tacgaaggtg
     1561 cgagggtgcg agggtgcgag ggtgcgaggg tgcgaggggg caagggtgcg agggtgcgag
     1621 ggggcaaggg tgcgagggtg ctgagatgag ggtggcgggg aaggggattg cgtgtgccgt
     1681 ggcggccgta atgtggctgt cgtgcctggt gctggtcagc ggtcaggcgg caccgaccga
     1741 tgcggcgcca acggcggagc aggtgttcaa gaacgtccag gtcctcaagg gcattccggt
     1801 caacgagttc atggggacga tgggcgtgtt ctccgcggcg ctgggcatgt cgtgcgaaga
     1861 ctgccacgga gcgggcgacg ccgactgggg cgtcta
//
LOCUS       DBNGSH010000130         7953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_689141, whole genome shotgun sequence.
ACCESSION   DBNGSH010000130 DBNGSH010000000
VERSION     DBNGSH010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_689141"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="AC16CY_02955"
     CDS             <1..224
                     /locus_tag="AC16CY_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352766.1"
                     /translation="NADTLPAGGQDPIRRILLNSNGSAKRMLDVIQEKNTAQGKKPAT
                     RSDLRLSLGPDNQVFLLNKGDGTIRVLTP"
     gene            326..2995
                     /locus_tag="AC16CY_02960"
     CDS             326..2995
                     /locus_tag="AC16CY_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3352767.1"
                     /translation="MSTLRAGSQVGSYEILAPIGAGGMGEVYRARDTKLKRDVAIKVL
                     PGEFSRDPERLRRFRREAEVLATLNHPNIAQIYGLEETGETCCLVLEMVEGDTLSDRL
                     AHGAMPVDEALGTARQIAEALEAAHGHDIVHRDLKPANIKVTPDGRVKVLDFGLAKIT
                     GPAEAGHYGSVRAEAGRYAGDRSVRLQPDLSQSPTMTTPAMTQTGMILGTASYMSPEQ
                     AKGRAADARSDIWAFGCVLYEMLSGKPAFDGDTVVETLGAILKSEPDWRALPQTTPPA
                     VLSLLKRCLQKDRDRRTRDIADARFQIEEAPNEPASPVASTPLRSSRERLLWSALTLV
                     LAGALAALGITYARSAPADLPEMRLEIGTPPGASLDGFAISPDGRALVYQATVQGKNQ
                     LWLRPLDSETAQPLAGTENGLRPFWSPDSRSVGFFAGGQLKRIDIAGGLVQTLAEAPL
                     ALGGAWSGEGTILFTQSSVEPLYRVPASGGKAVAVTEVKAPHVGHRTPHFLPDGRRFL
                     FFAFGPPQSQGIYIGSLDSTETTRLIDAESAPIFAPPDYVLFARQGAVLAQRVNFETL
                     QTLGDPLPVARQVATPVAVVASVALSAAMAGPVAYRADAGERQLRWVDRSGNPVGLLS
                     GPDSAQPSYARLSPDRRTVALVRMVSGNNDVWLTETARDARQRFTSDPAREFDLVWSP
                     DGSRIIFSSARKGVIDIYERSVSGGGTETPVWESHESKEVYDWSSDGRWILFATQSPK
                     TAHDLWVLPMEGEKKPIAVAQTASEERNARFSPDGRWIAYESNESGRNEIYVQPFPGP
                     GGRSPISTRGGASPQWQRDGKGLFYVDQGTRVMSVPVAANGPRVEPGTPVALFTLSQG
                     ATYEAAPDGQRFLINEITKDPSPITILLNWKPM"
     gene            complement(3017..4969)
                     /locus_tag="AC16CY_02965"
     CDS             complement(3017..4969)
                     /locus_tag="AC16CY_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3352768.1"
                     /translation="MKYLRSAMWLLVGLSMATAAAAQTENGEWRSYSGDAGSTKYSPL
                     DQIDRTNVKSLRIAWRRPAVDPLLSGKDPKLQVPNNFRATPLMIHGVLYSPNGVGLVE
                     AFDPGTGKTMWIQEPPPGPNALRGTSTRGVAYWRGGSDERLFVQRGETLIALNAKTGQ
                     PYSSFGVDGVVNLRFGPGAEAPYSWSGAPRVCRDVVIVGASMSDSPPRKEATPGDVRG
                     YDVGTGKLRWTFHVVPRAGELGVDTWKDQSWQYTGHANLWSLISVDEELGYAYLPLTS
                     PTSDMYGGHRPGDNLFSDALVSVECQTGKRVWHYQLVRHDLWDYDLPAAPILADIMVE
                     GRRIRSVVQLTKQAFAFVFDRVTGQPVWPIEDRPVPQSKTPGEQTSPTQPFPTKPPAF
                     DRQGVSLDDLIDFTPELRQAALEIVKQYVIGPMFTPPSIKGSGPNDTRGTIQLPGSVG
                     GADWQGGAFDPDTGLLYVQSITGPFVADIVPGDPKTTNLRFTRGTREYPPAPLGLPLL
                     KPPYGRITAIDLNKGEIAWMVPNGDGPRDHPLLTPLNLPPLGNPGRSAPLATKTLLFV
                     GEGDPIMANTDRTPKSMPPSLAPGAGGRTFRAFDKATGQVLWETEFPAGTTGAPMTYM
                     FGGKQYIVVAIGSREHPAEYVALSLP"
     gene            complement(4997..5149)
                     /locus_tag="AC16CY_02970"
     CDS             complement(4997..5149)
                     /locus_tag="AC16CY_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352769.1"
                     /translation="MALTVLKVEVLPPRSHLLRDLKPGNILITASGVQLPSRLWKRYG
                     QQALGA"
     gene            5286..5681
                     /gene="mntA"
                     /locus_tag="AC16CY_02975"
     CDS             5286..5681
                     /gene="mntA"
                     /locus_tag="AC16CY_02975"
                     /inference="COORDINATES: protein motif:HMM:NF047752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system MntA family
                     adenylyltransferase antitoxin"
                     /protein_id="HIR3352770.1"
                     /translation="MEAALRDTVRTEPAVVAAYVFGSVASGTAGPLSDIDVALLVHEP
                     RERRAVCDRTMDALSRVLRTSRVDVISLADAPLPLRYRVVRDGRLVLRRDARLLERFV
                     AETILHYLDFKPLRDRAFAHMRGEILEGR"
     gene            complement(5771..7387)
                     /locus_tag="AC16CY_02980"
     CDS             complement(5771..7387)
                     /locus_tag="AC16CY_02980"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3352771.1"
                     /translation="MTYPGHGLSRRQFLGGAAAALGGAGLSSASVRAQARQGAPEAVV
                     DLGIVNGRIHTMDPANRVVSQVLIRNGRFAAVGNDVVKRGGGLRVVDLRGKTVIPGII
                     DAHNHIVLVGNRPGWHTPLEHVLTIPDAIAALKARAGAVPAGEFVTTVGPISAMQFDE
                     RRLPNLTELDAVGRPVYIQAAQGGARTNSAGKAWLEARGIAVAADGAIAGPALGMALQ
                     TLRKELLTADTRKRSALDALNYYARLGITTHRDSGAFHAEEPAGGIASENTYTMHDPF
                     HAIHREGRMTARLRIDFLHQDPPNANPPLPTLGPRLRNTFPRFGDDWMKTGGIGEFTG
                     GGVEGLRAIARAGWQGEDHSLNLANATTLVANREAVNKEIPIKDLRWVISHIPEFPKE
                     LADRANAIGIGVLVGWGPLRTGTNVGPPYRMLFDHGIKVGFHSDGGDITVINPWLNFY
                     TMITGRNLAGQPILGDQTLTREQTMRLATSMNKWFVWEDDLGSIEAGNHADLAVLDRD
                     YFTVPDEDIRRIRSVLTIVGGKIVHNDGVA"
     gene            complement(7435..>7953)
                     /locus_tag="AC16CY_02985"
     CDS             complement(7435..>7953)
                     /locus_tag="AC16CY_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3352772.1"
                     /translation="TRPMTEAERAYVTDVHRWDEAERGYSAIQSTRPQTVGYGLNDSP
                     AGLAAWILEKWRAWSDSGGDLDARFSREFLLTMLTLYWATETVTTSMRDYFDNRWHGA
                     DIPPGTRVGVPAGFANFDNNYVSEGRPPREWAERLYEVRRWTSMPRGGHFGPAEEPEL
                     LARDIAAFFKEI"
BASE COUNT         1320 a         2604 c         2631 g         1398 t
ORIGIN      
        1 tcaacgccga cacgctgccg gccggcggcc aggatcccat tcgacggatc ctgttgaact
       61 cgaacggatc ggcgaagcgg atgctcgacg tcattcagga gaagaacacg gcgcagggca
      121 agaaaccggc gacacggtcc gacctgcggc tcagcctcgg ccccgacaac caggtgttcc
      181 tgctgaacaa gggcgacggc acgatccgcg tgttgacgcc gtgaagggcc gagccgcgcg
      241 tcctgcatgg cgactcggag aacgtatcgg cccgacctgg cgcggcggcg cgtgagctgc
      301 ctcgagagat acactgtccc cgcacatgag cacgctccgc gcgggttcgc aagtcggctc
      361 ctacgagatc ctggcgccca tcggcgcggg cgggatgggc gaggtatatc gagcgcgcga
      421 cacgaagctc aaacgcgatg tcgccatcaa ggtcctgccc ggggagttct cgcgcgaccc
      481 cgagcggctt cgccgttttc gccgggaagc cgaagtcctg gccactctca accatcccaa
      541 catcgcccag atctacggcc tcgaagagac cggcgagacc tgctgtctcg tcctggagat
      601 ggtcgagggc gacaccctca gcgatcgcct cgctcacgga gcgatgcccg tggacgaagc
      661 actcggaacg gcccgacaga tcgccgaggc cctggaagct gctcacggcc acgacatcgt
      721 ccaccgggat ctgaaacccg cgaacatcaa ggtcaccccc gatggacggg tgaaggtact
      781 cgacttcggc ctggcgaaga tcacgggtcc ggctgaagcc ggacactacg ggagcgttcg
      841 agctgaagcc ggacgctatg ccggcgatcg tagtgtccgg cttcagccgg acctctcgca
      901 gtccccgacg atgaccacgc cggcgatgac ccaaacgggg atgattctgg gtacggcgtc
      961 gtacatgtcg ccggaacagg ccaagggtcg agccgccgac gcacgctcgg acatctgggc
     1021 gttcgggtgc gtgctctacg agatgctgtc tggcaagcca gccttcgacg gcgacactgt
     1081 cgtcgagacg ctcggcgcga tcctgaagtc cgagccggac tggcgcgcgc tgccgcagac
     1141 gacgcctccg gcggtcctgt cgctgctgaa gcgctgcctt cagaaggatc gcgaccggcg
     1201 cacacgcgac atcgcggatg cgagattcca gatcgaggaa gcaccgaacg agcccgccag
     1261 cccggttgcg tccacgccgc ttcggagcag ccgcgaacga ctcctgtggt ccgccctcac
     1321 gctggtgctc gcgggcgccc tggcggccct gggcatcaca tacgcgcgga gcgccccagc
     1381 cgacctgccg gagatgcgcc tggagattgg cacgccgccc ggcgcgagcc tggatggctt
     1441 tgcgatttca cctgacggcc gcgcgctcgt gtatcaggcg accgtgcagg gaaagaatca
     1501 gctctggctc cggccattgg actcggaaac ggcccagcca ctcgcgggaa cagagaacgg
     1561 cttacgtccc ttctggtccc ccgacagccg gtcggtcggg tttttcgccg gtggccagtt
     1621 gaagcggatc gacatcgcgg gcgggctcgt gcagaccctt gcagaagcgc cgttggccct
     1681 tggtggcgca tggagcggtg agggcacgat tctgtttact caatcctcgg ttgaaccgct
     1741 gtatcgcgtg ccggccagtg gtgggaaggc cgtggccgtc accgaagtca aggcgccaca
     1801 tgtgggacac cgtacccctc acttcctgcc ggatggccgc cgctttctgt tcttcgcctt
     1861 cggtcccccg cagagccaag gcatctacat cggttcgctg gactccacgg agacgacgcg
     1921 cctgatcgac gccgaatcgg caccgatatt tgcgccgccg gattacgtgc tgttcgcgcg
     1981 gcaaggcgcg gtcctggcgc aacgtgtgaa cttcgagacg ctgcagacgc ttggggatcc
     2041 gctgccggtg gcccggcagg tggcgactcc ggtggcagtg gtggcgagtg ttgctttgtc
     2101 cgccgcgatg gcaggaccag tggcctaccg ggcggatgcg ggtgaacgtc agttgcgatg
     2161 ggtggaccgg tctggtaacc cggttggcct gctcagcggt cccgattcgg cgcagccaag
     2221 ctacgctcgt ctctcccctg accgtcgcac ggtggcgctc gtccgcatgg tcagtgggaa
     2281 caacgacgtg tggttgacgg agaccgcccg tgatgcccgc cagcggttta cgtcggatcc
     2341 ggcacgggag ttcgacttgg tctggtcgcc cgacgggagc cggatcattt ttagctcggc
     2401 tcgcaagggg gtaatcgata tctacgaacg atcggtcagc ggaggcggga cagagacacc
     2461 cgtgtgggaa tcccacgaaa gcaaggaagt ctacgactgg tcgtcggatg ggcgttggat
     2521 tctgtttgcg actcaaagcc cgaagacggc ccacgatctg tgggtgctac ctatggaggg
     2581 cgagaagaag ccaatagctg ttgcgcaaac ggcttctgaa gaaaggaacg cacggttctc
     2641 tccagacggc cggtggatcg cctacgagtc aaatgagagc ggacgaaacg aaatctacgt
     2701 tcagcccttt cccggcccgg gcggcaggtc gccgatttcc acgcggggcg gcgcgtctcc
     2761 tcagtggcag cgcgacggaa aggggttgtt ctatgtggat cagggcaccc gcgtcatgag
     2821 cgtgcccgtc gcggcgaacg gccccagagt cgagccgggc acgcccgtcg ccctgttcac
     2881 gctgtcgcaa ggggctacct acgaggccgc gccagacggc cagcgcttcc tgatcaacga
     2941 gatcacgaaa gatccctccc cgatcaccat acttctcaac tggaaaccga tgtaacggtc
     3001 gggccgcagt cgatcgtcac ggcaggctca gtgccacgta ctcggccgga tgctcccgcg
     3061 acccgatggc cacgacgatg tactgtttgc cgccgaacat gtacgtcatt ggcgcgccgg
     3121 tcgtgccggc gggaaactcg gtctcccaca gcacctgacc ggtggctttg tcgaacgccc
     3181 ggaacgtgcg tccgcccgcg ccaggcgcca gcgagggcgg catgctcttg ggagtgcggt
     3241 ccgtgttggc catgatcgga tcgccttcgc cgacgaacag cagcgtcttg gtcgccagtg
     3301 gagcgctccg gcccgggttc ccgagcgggg gcaggttgag tggagtgagc agcggatggt
     3361 ccctggggcc atcgccattg ggcaccatcc acgcaatctc ccccttgttg agatcgatcg
     3421 cggtgatccg gccgtacggg ggcttcagca acggaagccc gagcggcgcg ggcggatact
     3481 ctcgagtgcc gcgtgtgaat ctcaggttcg tggttttcgg gtccccagga acgatgtcgg
     3541 caacgaacgg accggtgatc gactgcacgt agagcaggcc cgtgtcgggg tcgaacgcgc
     3601 cgccctgcca gtcggcaccg ccgaccgagc caggcagttg aatcgtgccc ctggtgtcgt
     3661 tcggtccgct ccccttgatc gagggcgggg taaacatcgg gccgatgacg tactgcttga
     3721 cgatctcgag cgcggcctgg cgcagctccg gtgtgaaatc gatgagatcg tcgagcgaga
     3781 cgccctgtcg atcgaacgcc ggcggcttgg ttggaaaggg ctgcgtcggc gatgtctgct
     3841 cgccgggcgt cttcgattgc ggcacagggc gatcctcgat cggccacacg ggctgccccg
     3901 tgacgcgatc gaacacaaac gcaaacgcct gcttcgtgag ctgaacgacc gatcggattc
     3961 gcctgccttc gaccatgatg tccgccagaa tcggcgccgc cggcagatcg tagtcccaca
     4021 gatcgtgacg cacgagctgg tagtgccaga cgcgcttgcc ggtctggcac tcgacgctca
     4081 ccagcgcatc ggagaacagg ttgtcgccgg gacgatgtcc gccgtacatg tcgctcgtcg
     4141 ggctggtcag cggcagatag gcgtagccga gttcctcgtc gacgctgatc agcgaccaga
     4201 gattcgcgtg gccggtgtac tgccacgact ggtccttcca cgtatcgacg ccgagctccc
     4261 ctgcccgcgg caccacgtgg aaggtccatc gcagcttgcc ggtgccgacg tcgtagcctc
     4321 tcacgtcgcc cggcgtggct tccttacgcg gcggcgagtc gctcatcgac gctccgacga
     4381 tcacgacatc gcggcagact ctcggtgcac cgctccacga gtagggggcc tcggccccgg
     4441 gaccgaatcg gaggttcacc acaccgtcaa cgccgaaact ggagtagggc tgacccgtct
     4501 tcgcgttgag agcgatcagt gtctcgccgc gctgcacgaa gaggcgttcg tcgctgccac
     4561 cacgccaata ggccacaccc cgtgtgctcg tgccgcgaag cgcgttcggt ccgggcgggg
     4621 gctcctgaat ccacatcgtc ttcccggtgc cgggatcgaa ggcctctacg aggccgacgc
     4681 cgttcgggct gtagagcaca ccgtggatca tcaacggcgt cgcgcgaaag ttgttgggca
     4741 cctgcagttt gggatctttg ccgctgagca gcggatcgac ggccgggcgc cgccacgcaa
     4801 tccgaagact cttcacgttg gtgcgatcga tctgatcgag cggggagtat ttcgtgctgc
     4861 ccgcatcccc gctgtaactg cgccattcgc cgttctcggt ttgcgcggcg gcggcggtcg
     4921 ccatagacag gcccaccagg agccacatcg ccgatcgaag gtacttcatc accacctcct
     4981 ggcacgcgga gcgatcttac gctccaagcg cctgctgccc gtagcgtttc cacaatctgc
     5041 tgggcagctg gacgccggac gccgtgatga ggatgtttcc cggcttcagg tcgcggagaa
     5101 gatgcgatcg aggcgggagc acctcgactt tcagtaccgt cagggccacg gaactcgctc
     5161 tcccggaact cgtgttcacg aactatagcc gaacgtcaac caccgttgaa ctttgcccta
     5221 attcctctac aatgggcgct ggtgtgtgac acgtgaagca gggatcagag gcacgtcgtg
     5281 acgcagtgga ggcagcgctt cgcgacacgg tccggaccga gccggcggtc gtggcggcgt
     5341 acgtttttgg atcggtggcg tccggtacgg ccggtccact gagcgacatc gacgtcgcgc
     5401 tgctcgtcca cgaacctcgt gaacgtcgag cagtctgtga ccgcacgatg gatgccctga
     5461 gtcgcgtcct ccgaacgtcg cgggtcgatg tcatctcgct cgccgatgcg cccctgccac
     5521 ttcgctatcg cgtggtccgt gacggccgcc tggtgttgcg ccgtgatgcc aggctcctcg
     5581 agcgattcgt cgccgagacg attctccact atctggactt caagccgttg agagaccgcg
     5641 cattcgccca catgcggggc gagatcctcg aaggccgctg atcgatatct cgtggctatc
     5701 gagcgctatc tcgctcgtca gccgtgagag ggctgggcgc cctcctctcg aggcgggcac
     5761 gtacccgccg ctacgcgacg ccgtcgttgt ggacgatctt gccgccgacg atcgtcagga
     5821 ccgaccggat gcgcctgatg tcctcgtcgg gcaccgtgaa gtagtcgcga tcgagcacgg
     5881 cgaggtcggc gtgattgccc gcttcgatcg atcccagatc gtcttcccag acgaaccact
     5941 tgttcatcga cgtcgcgagc ctcatcgtct gctcgcgcgt cagcgtctga tcgcccagga
     6001 tcggctgtcc cgccaggttg cggcccgtga tcatcgtgta gaagttgagc cacgggttga
     6061 tgacggtgat gtcgccgccg tcggagtgaa aaccgacctt gatgccgtga tcgaacagca
     6121 tccggtacgg cggcccgacg ttcgtgccgg ttcgcagggg tccccacccg acgaggacgc
     6181 cgatgccgat ggcattcgcg cgatcggcca gctccttcgg gaactcgggg atatgcgaga
     6241 tgacccatcg caggtccttg atcgggatct ccttgttcac ggcctcgcgg ttggccacga
     6301 gcgtcgtggc gttggccagg ttgagcgaat ggtcctcgcc ctgccagccg gctctggcga
     6361 tcgctcgcag gccctcgacg ccgccgcccg tgaattcgcc gatgccgccg gtcttcatcc
     6421 agtcgtcgcc gaagcgcggg aacgtattcc gcagccttgg cccgagcgtc gggagcggag
     6481 gattcgcgtt cggcgggtcc tggtggagga agtcgattcg cagccgcgcc gtcatccgcc
     6541 cttcgcggtg gatcgcgtga aacgggtcgt gcatcgtgta cgtgttctcg ctcgcgatgc
     6601 ctccggccgg ctcctccgcg tggaaggcgc cagagtcgcg atgggtggtg atgccgagac
     6661 gggcgtagta gttcagcgca tcgagcgcgg atcgctttcg ggtgtccgcc gtgaggagct
     6721 ctttccgcag cgtctgaagc gccattccga gcgcgggacc tgcgatcgct ccgtcggcgg
     6781 ccacggcgat tccgcgcgcc tcgagccacg ccttgccggc gctgttcgtc ctcgcaccgc
     6841 cctgggcggc ttgaatgtac acgggccgtc cgacggcatc gagctcggtc aggttcggca
     6901 gccgccgctc gtcgaactgc atcgcggaga tcggtccgac ggtcgtgacg aactcgcccg
     6961 cggggacggc gccggctcgc gccttgagcg ctgcaatggc atcggggatg gtgaggacgt
     7021 gctcgagcgg cgtgtgccac ccgggccggt tgccgacgag gacgatgtga ttgtgcgcgt
     7081 cgatgatccc ggggatgacc gtcttccccc tgagatcgac gacccgcagg cctcctccgc
     7141 gcttcacgac gtcgttgccg acggcggcga agcgcccgtt gcggatcagg acctgagaga
     7201 cgacgcggtt cgccggatcc atcgtgtgga tcctgccgtt gacgatgccg agatcgacga
     7261 cggcctcggg cgccccctgc cgcgcctgtg cccgcaccga cgcggagctc aggccggcgc
     7321 cgccgagcgc cgcggccgct ccgccgagga actgtcttcg cgagagcccg tgaccgggat
     7381 atgtcatcga gtgagagccc gcattgtagc cccgttggcg gtgcgcgcga agcatcagat
     7441 ctccttgaag aaggccgcga tgtcacgcgc aaggagctcg ggctcctccg ccggaccgaa
     7501 gtgcccgccc ctcggcatcg acgtccaccg gcgaacctcg tacaggcgct ccgcccattc
     7561 gcgcggcggg cgtccttcag acacgtagtt gttgtcgaag ttggcaaatc ccgcgggcac
     7621 ccccacgcga gtgccgggtg gaatgtccgc cccatgccac cggttgtcga aatagtcgcg
     7681 catcgacgtc gtgacggtct ccgtcgccca gtagagcgtg agcatcgtga ggagaaactc
     7741 ccgcgaaaaa cgcgcgtcga ggtcgccgcc ggagtcgctc cacgcgcgcc acttctcgag
     7801 aatccaggcc gccagtccgg caggcgaatc gttcaaccca tagccgactg tttgtggccg
     7861 cgtagactgg atcgccgaat agccccgctc ggcctcatcc caacggtgca cgtcagtcac
     7921 gtatgcgcgt tcggcctcgg tcatcgggcg cgt
//
LOCUS       DBNGSH010000131        11298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2047620, whole genome shotgun sequence.
ACCESSION   DBNGSH010000131 DBNGSH010000000
VERSION     DBNGSH010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2047620"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..3017
                     /locus_tag="AC16CY_02990"
     CDS             66..3017
                     /locus_tag="AC16CY_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352773.1"
                     /translation="MKVLLAVTVATATAGTAFAQTSPASALRGPIGVEDRLDAPSAAT
                     VAIDRTRISTTDVPIFVRLTVDWTALEQASSTGQWSGLDDRLSAYTRRNMPILLAIAL
                     GSAATVESDAWTSSIQAVAEHLRGTVTGYQIEAAPTADPREYAFRLKLAAVRIKAIDP
                     QVFIAQATARPADAVWLSAVYAEGTAPYVDVAPLAAGDGRASNAADAVEAIVAAADPT
                     AARLLVGVPLGDPAQSADRLLKTTLTRLADAGSAGSTFEGDSDALSAALTAAARLTDL
                     LTGQLLLIDDVGVSLAIEANGGEVTASVPHRVLYNVSNGGTYLVYWNETGVNDRLAVR
                     LVDQGGRAPMVRDPIRGDVVAVQGFSWDATTKVSRMETPSSATPLVVDFNYGAANQFV
                     SRADVSAARALSVEEIVARHQRAQAAQSLTFTTVIADLLQKYHFRPTSVQVFDVVTEN
                     RYFLAPDSVEWEERSFSVNGAHWGPDRPDLPLLQAEKVLTLPLDLRLTAEYRYRLERT
                     ETVGERPSYVVAFEPNDAHHTSYRGWVWIDAGNFQRVKLQTIQTHLDGPIISNEEITT
                     YEPIGSANGPPVFLPVRLSAKQILLIAGRNVLLEKEQWFSGFRVDAPDFDAERQAARR
                     GPRVMFRDTAAGVRYLVKREDERVVKDRVTTSSKALAMGTTIDPTFAFPLPIFGINYL
                     NFDFRGTGGQMALLFGGVFALGNLQTPKVGRTPFDASVDFFAIAVPGTDQRFDATGER
                     VDERVLTIPMSTGVNLGYQFTPFQKVVVGYAFRYDAYFRDQNTADDFVIPSSTVSHGA
                     SVAYEFSRHGYRVGAGASTFLRTSWEAWGRPGDYEPGQRTYQRYSVAGGKDFLFGPFQ
                     SVHVGAGWYGGAQLDRFSSYQFGLFSEPRMHGVPAAGIRFPELALVHGSYSFNIYDIY
                     RFDLFLDHARGRDPTDDAVWQPVTGTGVAVTLKAPWNTMFTADVGKSFIPSIYRGTGS
                     WVLQVMFLKPF"
     gene            3027..3971
                     /locus_tag="AC16CY_02995"
     CDS             3027..3971
                     /locus_tag="AC16CY_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3352774.1"
                     /translation="MKADLHVHSYHSGYARHFHWLRARECYSTPEDVYRVAKARGMDL
                     VCITDHDSIEGCLEFLERHPDAPDFIAGEEISCLVPDAPGLRVHLGAIGMTESVHREA
                     QRLRDNVFDTAAYLRREGVFCALNHLFMLFDDQMAVESYVRMALTLAPGLETRNGAML
                     PAHNDFVDELASSLRGAGMSPVGIGGSDAHVLRYVGRTYTEAPGANREEFLANLVEGR
                     TEVGGDHGGRGRLLYEIYGSIANHWGGLLGFKRHEITLAERLASVVCSALLLPFQFVP
                     GLIAFRMKQNEQDRVDRCRRLWAEDVEHAPLVMGSPPR"
     gene            3979..5193
                     /locus_tag="AC16CY_03000"
     CDS             3979..5193
                     /locus_tag="AC16CY_03000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3352775.1"
                     /translation="MRRVVITGIGLQSSLGAGRERVWQNLVAGRCGIGQVTLFDSGGY
                     RSRLAAELADYDPSRAFTPLQRRRWSRSDQIAVLAAGEALDDSGLDRPSLDRDRVGVM
                     FGAGTSDLLRNEAYLKEVRCKGLARATPSKVVNFYSNTPVDVVGHRFGLSGPRHCVVA
                     ACSSSTIAIGFAADAIRAGKIDAALAGGADVLCRLTFSGFNALRLVDTQPCRPFDARR
                     AGMTIGEGAAVLVLEDFELARARGAPIYAELAGHSAVCEAYHPTSPEPDGLAIAATIQ
                     AALADARVPASDVDHINAHGTGTLHNDRAEARGFYRVFGDRARSIPVNAVKSMIGHCL
                     GAAGAVEAAVLALTVARGVIPPTVNHHETDPDCALDVVPNEARGQKVRCGLSTSLAFG
                     GNDAALVMRRVE"
     gene            5190..7643
                     /locus_tag="AC16CY_03005"
     CDS             5190..7643
                     /locus_tag="AC16CY_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3352776.1"
                     /translation="MSVIARLLVFARRRRWTVLGATAIFAVASAALASRGSFGTDVLA
                     MLPENSPSLQAFRRYVERFGTGEQLYILFERADGRAGIAEAGPEIEAYLTRLRQLAEI
                     ARVDAGVFDDSKDWTYVQDRTFALIGPEAARAAIGRFSDAGMRDALLRSRELLATPSP
                     EVRQLVQADPLGLLSLVREHFARDRVMARMDASRGYTSADGRSRLVIATPSQPPFDSA
                     FCRRLFGRLADIETEARHDASAGAGPAAPALRIAYAGGHRIAFETETIIRREATLNSV
                     TSLAAILTLLLAVFRSPWLFLVGAIPMSVAAIGSIAINSVISDELSAAATGTSALLFG
                     LGIDGLVLLYARYLEEVESGLPADTAIARLGSAGVSMFLGCVTTAATFLGLTWIDLPG
                     LRELGRLVGVGMLVGAPLTLLLVAALLPSRVTRPRALSASGLAAFVRRRRWPILIGAT
                     VTTVVALPMLRTLELDLRLQRLQPQTPAVALQEDLQERFGLGGDVVIALAEGPDLDSL
                     VETDRQFEATLRAEAPSLAVSGPSRLVPPQGEQDETGRILASLTPDIEAIRSRLRAAA
                     ADAGFRPGAIDPFLERLPRLLDPSQRLTYQGYVDHGLTDAISRYAVRGPDGFMTASYV
                     EVTDTGDLATVSRAADEVGRGLTLTGMSIANADLAARFGPQFGIALAAGCVAVFLLIL
                     ITFRRVGPTILAIAPTAVGLIWAAALLTRFDVSLDLFSIFAVLMLIGIGVDYGIHLVH
                     RSTTEPGNLDLALARIAPANLVAAGIAVLGCGSLVTSTYPPLRSLGLVTVIGLITCLV
                     TAVLVLPALLMVMAPRKGQ"
     gene            7680..9014
                     /locus_tag="AC16CY_03010"
     CDS             7680..9014
                     /locus_tag="AC16CY_03010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3352777.1"
                     /translation="MSGPLRFDCDVIVVGGGPAGSAASAWLARAGHAVALFERDRFPR
                     FHIGESLLASVNDVLAAIGADTLVRQAGFPQKWGATFVLGDGRAERYADFSASRGVST
                     PQTWQVPRATFDNLLLRHAEASGVLVHERHRVLGVEFDADGVTAFVQDEQKEDGRERR
                     HVRARAIVDASGRGSVLSRKFNLRVDEPRLANIAIFSHYSGVPRQAGRRGGDIRIVAR
                     ADLGWFWLIPISDQLMSVGVVLPRAAFAARTEKDHAVLLDQAIAETPVVASLLSAARR
                     EWPVRVEKDFSFGTRAYAGDRWLLAGDAGSFLDPVFSTGVAIALESGLEAGQAISVGL
                     RTGDLSKARFGRYARRQHQRYRSFRRFVLGFYTPAFRDLFFSEDPPACIFTSLVTVFA
                     GYWRPSVVTRLWVAVFFLLVRLQGWFRFAPSYRPQTALETDGDSAPAVALPK"
     gene            9011..9748
                     /locus_tag="AC16CY_03015"
     CDS             9011..9748
                     /locus_tag="AC16CY_03015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3352778.1"
                     /translation="MSQPPLPELSHYWANADERQRVVNSLFARSARHYDRACDIMSFG
                     TGRTYRRTALERAGVEPGMRVLDVGTGTGQLAQEAARLVGSTGSVIGLDPSREMMTVG
                     PRTDSISFVQGLGEFLPLPDRYFDFVTMGYALRHVRDLDQTFVEYFRVLKPGGHALLL
                     EITRPRSAIGYAIMRAYCGTVVPSLARLGTRSADAARLMRFYWDTIANCVPPEVVLAS
                     LRRSGFAAAERRCTAGIFSEYIATREG"
     gene            complement(9765..10190)
                     /locus_tag="AC16CY_03020"
     CDS             complement(9765..10190)
                     /locus_tag="AC16CY_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352779.1"
                     /translation="MATFRRLIFFAATSLLLTPLSLNAFADAVEKQVLDYLHRSKLCA
                     SVNFYDLRVDWRDGIVENEYDREQVRKDDPLTFRDVDLKKDGLNLKVRSPSTRGESIK
                     VSIILSASVKSPTGEFVNQDELGKALGFFTRPCDQESKP"
     gene            complement(10222..>11298)
                     /locus_tag="AC16CY_03025"
     CDS             complement(10222..>11298)
                     /locus_tag="AC16CY_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3352780.1"
                     /translation="AAYGEGETPLMLAARSGNLESVKLLLEAGADVNTAEKFRGQTAL
                     MLAASENHGVVVKALLDAGAQVNTRTAEYTFQALTGGAGGIIHDRPQGSLTALILAAR
                     QGARDAAAHLLDAGADVDLAEPQYGFTALQTAIFNGHYALARLLLEKGADPNDGSLYI
                     AMEMRNLATYSNRPNPPDVEDGVSHLDVAKLLIEKGANPNAPYIKSIPPRQAQGEINV
                     LPGATPLYRASRAVDLASVQLLLGAGADASQAIKDGSTPLMAASGLGAPRGGSEEVTE
                     AGERSDPVEVVKALVDKGANVNAANDTGMTPLHYAAQRGSDRIVEFLAGKGARFDVKN
                     KQGRTPADLARGKTAALISKLSAQ"
BASE COUNT         1690 a         3746 c         3786 g         2076 t
ORIGIN      
        1 cggctcgaaa cgatcctggg gcgcgccacg ttcggaccac tggacacggc actcggggaa
       61 acgcagtgaa agtcctgctg gctgtcacgg ttgcgaccgc gaccgctggg acagcctttg
      121 cgcaaaccag ccccgcgtcg gcgctccgtg gtccaatcgg cgtagaggac cggctcgatg
      181 ccccgtcggc tgcgaccgtc gccatcgaca ggacccgcat ctcgacgacc gacgtcccga
      241 tcttcgtcag gctcaccgtg gattggacgg cactggagca ggcgtcgtcc accggccaat
      301 ggtccgggct cgacgatcga ctcagcgctt acacgcggcg gaacatgccg atcctgcttg
      361 cgatcgcgct tggcagtgca gcgacggtcg agagcgacgc gtggacgtcg agcattcagg
      421 ccgttgccga acacctgcgc ggcacagtca ccggatatca aatcgaagcg gcgcctaccg
      481 ccgatccacg cgagtatgcg tttcgcctca agctcgcggc tgtccggatc aaggcgatcg
      541 acccgcaggt gttcatcgcc caggccacgg cgcgcccggc ggatgccgtg tggctcagcg
      601 ccgtatacgc cgagggcacg gcaccctacg tggacgtcgc gcccctcgcc gctggtgacg
      661 gtcgtgcgtc gaatgccgcg gacgccgtcg aggcgatagt ggctgccgcc gatccgactg
      721 ctgcgcggct cctggtcggc gtgccgcttg gagatcccgc ccagagtgcc gaccggctgc
      781 tgaagaccac gttgacccgc ctcgcggacg cgggttcagc gggatcaacc ttcgagggtg
      841 acagcgacgc gttgtctgcc gcgctcacgg cggccgcgcg actgacggat ctcctcacgg
      901 gtcagcttct ccttatcgac gatgtcgggg tctcgcttgc gatcgaggcg aacggcggcg
      961 aggtgacggc aagcgtgccg catcgcgtgc tctacaacgt ctcgaacgga gggacctatc
     1021 tcgtctattg gaacgagaca ggcgtgaacg atcggctggc cgtccggttg gtggatcagg
     1081 gcggccgcgc gccgatggtg cgcgacccca ttcgcgggga cgtcgttgct gtgcagggct
     1141 tttcctggga cgcgaccacg aaggtgtctc gcatggagac gccatcgtct gcgacgccgc
     1201 tcgtcgtcga cttcaactac ggcgccgcga atcagttcgt cagccgcgcg gacgtgtcgg
     1261 cggcgcgggc gctgagcgtc gaggagattg tcgcgcgcca ccagcgcgcg caggcggcgc
     1321 agtcgctgac attcacgacc gtcatcgccg atctcctgca gaagtatcac ttccgcccga
     1381 cgtcggtgca ggtcttcgac gtcgtgaccg agaaccgcta cttcctggcg ccggacagcg
     1441 tcgagtggga ggagcggtcc ttctcggtga acggcgcgca ctggggcccc gaccggccgg
     1501 acctgccgct gctgcaggcg gagaaagtgc tgacgctgcc gctcgacctt cgcttgacgg
     1561 ccgagtaccg ctaccgtctc gagcggacgg agaccgtcgg cgagcggcca agttacgtcg
     1621 tcgcgttcga gccgaacgac gcgcaccaca caagctatcg cggttgggtc tggatcgacg
     1681 ccggcaattt ccaacgcgtg aagcttcaga cgattcaaac acacctcgac ggccccatca
     1741 tctcgaacga ggagatcacg acctacgagc cgatcggatc cgcaaatggc ccgccagtct
     1801 ttctacctgt gcggctgtcg gccaagcaga tcctgctcat cgctggacga aacgtgctgc
     1861 tcgagaagga acaatggttt tccgggttcc gcgtggacgc gccggacttc gatgccgagc
     1921 gtcaggcggc gcggcgtggg ccgcgcgtga tgttccgcga caccgccgcc ggcgtacgct
     1981 acctcgtgaa gcgcgaggac gagcgcgtgg tcaaggaccg cgtgacgacg tcgagcaagg
     2041 cgctggcgat gggcacgacg atcgacccga cgttcgcgtt tccgttgccg atcttcggga
     2101 tcaactacct gaacttcgac ttcaggggca cgggagggca gatggccctg ctgttcggtg
     2161 gcgtgttcgc gctcgggaat ctgcagacgc cgaaggtcgg caggacgccg tttgacgcga
     2221 gcgtggactt cttcgcgatt gcggtgccgg ggaccgacca gcgattcgac gcgacgggcg
     2281 agcgagtgga cgagcgggtg ctgacgattc cgatgtccac tggcgtgaat ctcggttacc
     2341 agttcactcc cttccagaag gtcgtcgtcg ggtacgcgtt ccggtacgat gcgtactttc
     2401 gcgaccagaa tacggcggac gacttcgtca ttccctcgtc caccgtttcg catggcgcga
     2461 gcgtggcgta cgaattcagc cgccacgggt atcgagtagg agcaggagcg tctacgttcc
     2521 tccggacctc atgggaggcc tggggacggc ccggtgacta cgagccgggt cagcggacgt
     2581 accagcgcta ttcggtggcc ggcggaaagg acttcctgtt cggcccgttc cagtcggttc
     2641 acgtcggcgc cgggtggtac ggaggcgcgc agctcgatcg tttcagctcg taccagttcg
     2701 gcctgttctc cgagcctcgg atgcacggcg tgccggcggc cgggatccgc tttccggagc
     2761 tcgcgctcgt ccacggttcc tactcgttca atatctacga catctacagg ttcgatctct
     2821 tcctggacca cgccaggggc cgcgatccga ccgacgacgc cgtgtggcag cccgtcaccg
     2881 gcacgggagt ggccgtgacg ttgaaggccc cttggaatac gatgttcacg gcggacgttg
     2941 gcaagagctt cataccgagc atctatcgcg gaaccggctc gtgggtcttg caggtcatgt
     3001 tcctcaagcc gttctaatcc gtttccatga aggccgacct gcacgttcac tcctatcact
     3061 ccggatacgc acggcatttt cactggttgc gcgcgcgcga gtgttactcc acacccgagg
     3121 acgtctaccg cgttgccaaa gcgcggggca tggacctcgt gtgcatcacg gatcacgaca
     3181 gcatcgaagg ctgtctcgag ttcctcgagc ggcaccccga cgcaccggac ttcatcgccg
     3241 gcgaagagat ctcgtgtctc gtcccggacg cgccggggtt gcgggtccat ctgggcgcga
     3301 tcggcatgac cgagtcggtg catcgcgagg cgcagcggct gcgcgacaac gtcttcgata
     3361 cggccgccta cctccgccgc gaaggcgtct tctgcgccct caaccatctg ttcatgctgt
     3421 tcgacgatca gatggccgtc gagtcgtacg ttcggatggc gctcacgctg gcaccgggcc
     3481 tcgagacgcg caacggcgcg atgcttccgg cgcacaacga tttcgtggac gagctcgcct
     3541 cgtcgctgcg gggggccggg atgtcgccgg tgggcatcgg cgggagcgac gcgcacgtgt
     3601 tgcggtacgt cggccggacc tacaccgagg cgcccggtgc caaccgtgag gaattcctgg
     3661 ccaacctcgt cgaggggcgc acagaggtcg gcggcgatca cggcggccgg gggcggctgc
     3721 tctatgaaat ctacgggtcg attgccaatc actggggcgg cctgctcggt ttcaagcgcc
     3781 acgagatcac tctcgccgaa cgtctcgcgt ctgtcgtgtg ctcggcgctg cttctgccgt
     3841 tccagttcgt cccgggcctc atcgcgtttc gcatgaaaca gaacgagcag gaccgcgtgg
     3901 accggtgccg gcgcctctgg gccgaggacg tcgagcacgc gcccctcgtg atgggatcgc
     3961 cgccacgctg acgcatcgat gcgacgcgta gtcattaccg gcatcggcct gcaatcgtcg
     4021 ctcggtgcgg ggcgcgagcg tgtgtggcag aatctcgtcg ccggacgctg cgggatcggt
     4081 caggtcacgc tgttcgattc tggcggctac cgcagtcggc tggcggccga gttggctgac
     4141 tacgatccgt cgcgtgcctt tacgccgctc cagcggcggc gatggtctcg gagcgatcag
     4201 attgccgtgc tcgccgcggg ggaggcgctc gacgattccg gccttgaccg gccatcgctc
     4261 gaccgcgatc gcgttggtgt gatgttcggc gccgggacga gcgatctcct tcgcaacgaa
     4321 gcctacctca aagaggtacg ctgcaaaggc ctcgctcgcg caaccccgtc aaaggtcgtc
     4381 aacttctact cgaacacgcc ggtagacgtc gtcggacatc gtttcgggct gtccggccca
     4441 cgccactgcg tcgtggccgc gtgctcgtcc agcaccatcg ccatcggctt cgccgcagac
     4501 gccatccggg cagggaagat cgacgcggcg cttgcgggcg gggccgacgt cctgtgccgg
     4561 ctcaccttca gtggcttcaa cgcgctgcgg ctcgtggaca cccagccgtg ccggccgttc
     4621 gacgccaggc gcgccggtat gacgattggc gaaggcgccg cggtgctcgt gctcgaggac
     4681 ttcgagctcg ccagggcccg aggtgcgccc atctacgccg agctcgccgg gcacagcgcg
     4741 gtgtgtgagg cctaccatcc gacctcgccc gagcctgatg ggctcgcgat cgcggcgacg
     4801 attcaggccg cactcgctga tgccagggtc ccggcgagcg acgtcgacca catcaatgct
     4861 cacggaacgg gcacgctgca caacgaccgc gcggaggctc gcggcttcta tcgcgtgttc
     4921 ggcgaccgcg cgcggtcgat accggtgaac gccgtgaagt cgatgatcgg acactgcctc
     4981 ggggctgccg gtgccgtcga ggcggccgtg ctcgcgctga cggttgcgcg cggcgtcatt
     5041 ccgccgacgg tgaaccatca cgagaccgat ccggactgcg cgctcgacgt cgtccccaac
     5101 gaagcccgcg gacagaaggt gcggtgcggc ctgtcgacgt cgctggcgtt cggtggcaac
     5161 gacgccgcgt tggtgatgcg gcgggttgag tgagcgtcat cgcgaggtta ctggtcttcg
     5221 cccgccgccg gcggtggacg gtgctcggcg ccacggcgat cttcgccgtg gcatcggcag
     5281 cactggcgtc gcgtgggtca ttcggcaccg acgtgctggc catgctgccc gagaacagcc
     5341 cctctcttca ggccttccgc cggtatgtcg agcgattcgg gaccggcgag cagctctata
     5401 tcctcttcga acgggccgat ggccgcgcgg gcattgccga ggcggggccc gagatcgagg
     5461 cctatctcac gcgcctgcgg caactcgccg aaatcgccag ggtcgacgct ggtgtcttcg
     5521 acgatagcaa ggactggacc tacgttcagg atcgcacctt cgcgctgatt gggccggaag
     5581 cggcgcgcgc cgccatcggc cgtttcagcg atgccggcat gcgcgacgcg ttgctccggt
     5641 cgcgcgagct gctggccacg ccgtcaccgg aggttcggca actcgtgcag gcggatccac
     5701 tcggcctgct gtcgctcgtc cgggaacact tcgcgcgcga tcgcgtgatg gctcgcatgg
     5761 acgccagccg cgggtacaca tcagccgacg gtcgcagccg cctggtcatc gcgacgccaa
     5821 gccagccccc tttcgacagc gcgttctgtc gccgcctgtt tggccgattg gcggatatcg
     5881 agactgaagc ccgtcacgac gcttctgcag gagcgggacc ggccgctccg gcgttgagga
     5941 ttgcctacgc cggcggccat cggatcgcgt ttgagacgga aacgatcatc cgccgcgagg
     6001 cgacgctgaa cagcgtgacg tcccttgccg cgatcctgac tctgctgctt gcggtgttca
     6061 ggagcccgtg gctgttcctc gtcggcgcca ttccaatgtc ggtcgcggcg atcggctcga
     6121 tcgcgatcaa cagcgtgatc agcgacgaac tgtcggcggc ggcgacaggc acatcggcgc
     6181 ttctctttgg cctcggcatc gacggactcg tcttgctgta cgcgcggtac ctcgaagaag
     6241 tcgaatcggg gctgcctgcc gacaccgcga ttgcacggct cggcagcgct ggcgtcagca
     6301 tgttcctcgg ctgcgtcacg acagccgcca cattcctggg tctcacctgg atcgatctac
     6361 ctggcctgcg ggaattgggg cgcctggtgg gcgtcggcat gctcgtggga gcgccgctca
     6421 cgctgcttct cgtcgccgcc ctgcttccgt cgagggtcac ccgtccgcgc gcgctgtcgg
     6481 cgtcagggct cgccgcattc gtccgccgtc gccgctggcc gatcctgatc ggcgccactg
     6541 tcacaacggt cgttgcgctg ccgatgctcc gtacgctcga gctcgacctc aggctgcagc
     6601 gccttcagcc gcagacgcca gccgtggcgc ttcaagagga tcttcaagag cgcttcgggc
     6661 tcggtggcga cgtcgtcatc gcgctcgccg aggggccgga tctcgactct cttgtcgaga
     6721 cggatcggca gttcgaggcg acgctccggg ccgaggcgcc ttcgctggcg gtctcgggac
     6781 cgtcgcgcct cgtgccgccg cagggagagc aggacgagac cggccgtatt ctggcctcgc
     6841 tcacgccgga catcgaggcc atccgatcgc ggctccgggc ggctgccgcc gacgcggggt
     6901 ttcgacccgg agcgatcgac ccgttcctcg agcggctgcc gcgcctgctc gatccgtccc
     6961 aacgcctgac ataccaggga tatgtcgatc acggtctcac ggacgcgatc tcgcgctacg
     7021 ccgtgcgggg gccggacggc ttcatgaccg cgtcctacgt cgaggtgact gacactggtg
     7081 atctcgcgac cgtgtcgcgg gcggccgacg aggttggccg cggccttacg ctgacgggca
     7141 tgtcgattgc caacgccgat ttggccgcca ggttcggccc gcaattcggg atcgcgctcg
     7201 cggccgggtg cgtggccgtc tttctgctga tcttgatcac gttccgtcgg gtggggccga
     7261 cgatcctcgc gatcgcgccg acggccgtcg ggttgatttg ggcggccgcg ctgctgacgc
     7321 gattcgacgt cagcctggat ctcttcagca tatttgcggt gctcatgctg attgggatcg
     7381 gcgtggacta cggcatccac ctggttcacc ggtcgacgac cgaacctggc aatctcgacc
     7441 tcgcgctcgc gcggatcgcg ccagccaacc tcgtggcagc cgggatcgcg gttctcgggt
     7501 gcggatcgct cgtgacgtcc acatatccgc cgctgcgatc gctcggtctc gtgacggtga
     7561 ttggcctcat cacgtgcctc gtcacggctg tactggtctt gccggcgttg ctgatggtga
     7621 tggccccacg gaagggacag tgacgcgatc gcgcccgcag cggtggcata ctgtgggccg
     7681 tgtccggccc cttgcgcttc gattgcgacg tgatcgtcgt cggcggcgga cccgcgggtt
     7741 cggccgcgtc cgcgtggctc gcgcgcgcgg gccacgcggt ggcgctcttc gagcgtgatc
     7801 ggtttccccg cttccacatc ggcgagtccc tgctcgcgtc ggtcaacgat gtgctcgccg
     7861 cgatcggtgc cgacacgctc gtgcgccagg cagggttccc gcagaagtgg ggcgccacgt
     7921 tcgtgctcgg cgacggacgc gccgagcgtt acgcggactt cagcgcgtct cggggggtct
     7981 cgactccgca gacgtggcag gtgcctcgcg cgacgttcga caacctcctg ctgcgccacg
     8041 ccgaggcaag cggcgtcctc gtccacgaac gccaccgagt gctcggcgtc gagttcgacg
     8101 cggacggcgt caccgcgttc gtgcaggacg agcagaagga ggacggccgc gagcggcggc
     8161 atgttcgggc gcgggcgatc gtcgatgcct cgggccgcgg gtccgtgctc tcgcgcaagt
     8221 tcaacctgcg ggtcgacgaa ccgaggctgg cgaacatcgc gatcttctcg cactattccg
     8281 gagtcccacg gcaggccggc cgccgcggcg gtgatatccg catcgtcgcg cgcgctgatc
     8341 tcgggtggtt ctggctgatt ccgatctcgg accaactcat gagcgtcggc gtggtgctgc
     8401 ctcgtgcggc cttcgccgca agaaccgaga aggatcatgc cgtgctgctc gatcaagcga
     8461 tcgcggaaac ccccgtcgtg gcatcgctct tgtcggccgc gcggcgcgag tggccagtgc
     8521 gcgtcgagaa ggacttctcg ttcggaacgc gcgcgtacgc cggtgaccgg tggctgctcg
     8581 ccggcgacgc cggctcgttt ctcgatcccg tgttctcgac cggcgtcgcg atcgccctgg
     8641 aatcgggcct cgaggccggt caggccatat ccgtcggcct ccggactggc gacttgtcga
     8701 aggcgcggtt cggtcgatac gcgcgccggc aacaccagcg gtaccgatcg ttccgccgct
     8761 tcgttctagg attctacaca ccggcctttc gtgatctgtt cttcagtgaa gatccgcccg
     8821 cttgcatctt cacgtcgctc gtcaccgtgt tcgcaggtta ctggcggccg tcagtcgtca
     8881 ctcgtttgtg ggtagccgtg ttctttctcc tcgtacggct gcagggatgg ttccggttcg
     8941 ctccatcgta ccggccacag accgcactcg aaaccgacgg cgacagcgcg ccggcggtgg
     9001 ccctcccaaa atgagtcaac cgccactacc ggaactgagc cattactggg caaatgcgga
     9061 tgaacggcag cgcgtcgtca attccctgtt tgcgagatcg gcacggcact acgaccgcgc
     9121 gtgcgacatc atgtcgttcg ggacaggacg gacgtatcgg aggacggctc tcgaacgggc
     9181 aggtgtcgag ccaggcatgc gcgtactgga tgtagggacg ggcacagggc agctggcgca
     9241 ggaggcggcg cggctcgtcg gctcgaccgg cagtgttatc gggcttgacc cctcccgcga
     9301 gatgatgacc gttggtccgc gcacggactc aatcagcttt gtgcaggggc tcggcgagtt
     9361 cttgccgctg cccgatcggt acttcgactt cgtcaccatg ggatacgcgc tgcggcacgt
     9421 gcgcgatctc gatcagacgt tcgttgaata cttccgcgtg ctgaagcctg gcggccacgc
     9481 gctgctgctg gagatcacca gacctcggtc ggctatcggc tatgccatca tgcgggcgta
     9541 ctgtgggacg gtcgtaccga gcttggcgcg actcggcacg cgcagcgcgg acgcggcgcg
     9601 gctcatgcgc ttctattggg ataccatcgc caactgcgtg ccaccagagg tcgtcctcgc
     9661 ctcgctccgg cggtccggct ttgctgccgc cgagcgcaga tgcaccgcag gcatcttcag
     9721 cgagtacatc gccactcgag agggatgatc ttcaggcgga tgcctcaggg cttcgattcc
     9781 tggtcacaag gacgggtaaa gaagccgagc gcctttccca gctcatcctg gttcacgaat
     9841 tctccggtcg gtgacttcac cgatgcggaa aggatgatcg acaccttgat ggactcgccg
     9901 cgagtgcttg ggctcctcac cttgaggttg agaccgtcct tcttgagatc aacgtcgcgg
     9961 aaggtcagcg ggtcgtcctt tcggacctgc tcgcgatcgt actcgttttc aacgatccca
    10021 tcccgccaat ctacccgcag atcgtaaaag ttgacggaag cgcacagttt agagcgatga
    10081 aggtagtcga gcacctgctt ctcaacggca tccgcgaagg cgttcaggga gaggggtgta
    10141 agcagcaggc ttgtggccgc gaagaagatg agtcgtctga aggtcgccat ggcccgtacg
    10201 cgtccgtcca acgcttgctc gctattgggc gctgagcttg ctgatgaggg ccgccgtctt
    10261 gccgcgcgcg aggtccgctg gcgtgcgccc ctgcttgttc ttcacgtcga agcgggcccc
    10321 tttgccggcc aggaattcga cgatgcgatc cgaccctcgc tgcgcggcgt agtgcagcgg
    10381 cgtcatgccg gtgtcgttcg cggcgttcac gttcgcgccc ttgtcgacga gcgctttcac
    10441 gacctccacc gggtcgctgc gctcgccggc ctcggtgact tcctcgctcc cgccgcgcgg
    10501 agcgccaagg cctgaggcgg ccatcaaggg agtcgacccg tccttgatgg cctgagacgc
    10561 gtccgcgccg gcgccgagga gcagctgcac ggacgccagg tccacggcac gcgacgcccg
    10621 gtagagcggc gtcgcgccgg gcaacacgtt gatctcgccc tgcgcctgcc ggggcggaat
    10681 cgacttgatg tacggcgcgt tcggattggc gcccttctcg atcagcagct tcgccacgtc
    10741 gagatgactg acgccgtctt cgacgtccgg cggattcggc cgattgctgt aggtcgccag
    10801 attccgcatt tccatcgcga tgtagagcga cccgtcgttg ggatcggcac ccttctcgag
    10861 gagcagcctg gcgagcgcgt agtggccgtt gaagatcgcc gtttgcaggg cggtgaaccc
    10921 gtactgcggt tcggcgagat cgacgtcggc accggcgtcg agcagatgcg ctgccgcgtc
    10981 tctcgccccc tggcgcgccg cgaggatcaa cgccgtgagg ctgccctgcg ggcggtcgtg
    11041 gatgatgccg ccggcccctc ccgtcagcgc ctggaacgtg tattcggcgg tccgcgtatt
    11101 gacctgcgcg ccggcgtcca ggagcgcctt cacgacgacg ccgtgattct cggacgcggc
    11161 cagcatcagc gccgtctgtc cccggaactt ctctgcggtg ttgacgtccg ctccggcctc
    11221 gagcagcagc ttcaccgact cgaggtttcc cgatcgtgca gcgagcatca gcggcgtctc
    11281 gccctcgccg tacgccgc
//
LOCUS       DBNGSH010000132         2671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3405717, whole genome shotgun sequence.
ACCESSION   DBNGSH010000132 DBNGSH010000000
VERSION     DBNGSH010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3405717"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16CY_03030"
     CDS             complement(<1..266)
                     /locus_tag="AC16CY_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3352781.1"
                     /translation="MGSFLRHSMGLSAWKVFRESRWALRVNPSSGNLHPTEAYVVLGG
                     RVCHYASREHALEERAVLSREAWTAFFGERDGFLVALTSIHWREA"
     gene            complement(495..755)
                     /locus_tag="AC16CY_03035"
     CDS             complement(495..755)
                     /locus_tag="AC16CY_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352782.1"
                     /translation="MGDAGEGLVDAESRLAERMEEREQERRNAKQSVKGLDPERVRQI
                     ESLRLARTEAQRQMEGTAHAARKQQLTQAIAEIDKRLAVLSK"
     gene            complement(821..1240)
                     /locus_tag="AC16CY_03040"
     CDS             complement(821..1240)
                     /locus_tag="AC16CY_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3352783.1"
                     /translation="MTRAFVADASVAIGWVHPAQATKQTAAMLEAIADGATLEVPALW
                     PLEVANALIVLVRRRRLAEDECQAGLGWLRGLRVRLDHEMTALAFSRLSELAAAHDLS
                     VYDAAYLELAQRRRLVLGCKDGPLRSAAKRAGVALWA"
     gene            complement(1237..1494)
                     /locus_tag="AC16CY_03045"
     CDS             complement(1237..1494)
                     /locus_tag="AC16CY_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3352784.1"
                     /translation="MATVTAFEAKTRFGELLDRVSKGEEVVITRHDKAVARLVPEGAQ
                     RHDEVRRSVEGLRDLQQRIGRRSKAKLTDRDVRAAIDHGRP"
     gene            1576..>2671
                     /locus_tag="AC16CY_03050"
     CDS             1576..>2671
                     /locus_tag="AC16CY_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3352785.1"
                     /translation="MPRSSPLDLFDPVVREWFTRSFASPTTPQKLGWPAIARGESTLM
                     LAPTGSGKTLAAFLWCLNRLMFSPAPPPKSRCRVLYISPLKALAVDVERNLRAPLAGI
                     AAVARGDGRDVTSPEIAIRTGDTPAPDRARFGREAADILITTPESLFLLLTSNARARL
                     ATVDTVIIDEIHALVGGKRGAHLAVSLERLEAIRPAGAPPLQRIGLSATQRPLEEVAR
                     FLGGAAPPARTKRPRARKPAGRRSAPATTVEAELREGLEGTNGAIEYRPVTIVDAAEK
                     KPLALRVEVPVEDMARPVPDAPKDAPVPQGPVHPSIWTAVHPRLLDLIRAHRSTLLFV
                     NSRRLAERLAGALNDLAGEPLVRSQHGSLSH"
BASE COUNT          419 a          925 c          873 g          454 t
ORIGIN      
        1 gcctctcgcc agtgaatgga ggtgagcgcc acgaggaagc catcgcgctc gccgaagaag
       61 gccgtccagg cctcccgcga caggacggcg cgctcttcga gcgcgtgctc gcgggatgcg
      121 tagtggcaga cgcggccgcc aaggacgacg tacgcctcgg ttggatgcag gttgccgctc
      181 gacgggttga cgcggagcgc ccacctcgat tcacggaaga ccttccacgc ggagagaccc
      241 atggaatgcc gcaggaacga gcccaccgat gcttcgtcga tctcagcggc cttccgcggc
      301 agctcgaaga tccggtcgaa cgggacactc gacccaaggt cgttgtgcgg gaggggaatc
      361 aggcgtgcgc cgtcgtagcg gcggaacgga tcgggctgag tcgcccagtc caggtagccg
      421 agcgaggggg caaaccggcc gaaatcgtgc ttggtggact cgtggtactc gagggcaatc
      481 agggcccgat gtccctattt cgagagcacg gccagccgct tgtcgatctc ggcaatcgcc
      541 tgcgtgagct gttgtttgcg cgcggcgtgc gcggtgccct ccatctggcg ctgcgcctcg
      601 gtgcgcgcca ggcgaagcga ttcgatctgt cgcacgcgct cggggtcgag gcccttcacg
      661 ctttgtttgg cattgcgtcg ttcttgttcc cgctcttcca tccgctcggc gaggcgagac
      721 tccgcgtcga cgagtccttc tccagcgtca cccatgtctt cgattatcaa caaacagacg
      781 tctcatgtgc aatcgaaagg cgcgtacccg tggttggtgg tcaagcccag agagccacgc
      841 ccgcgcgttt cgccgcggag cgcaagggac cgtccttgca gcccaacacg agcctgcgcc
      901 gctgggccaa ttccaggtac gcggcatcgt agactgacag atcgtgggcg gcggccaact
      961 cggacaggcg cgaaaacgcc agtgccgtca tctcgtgatc gagtcgcacg cgaagtccac
     1021 gcagccaacc gagccccgct tgacattcgt cctccgcgag ccttcttcga cgaacgagga
     1081 cgatgagcgc gttggccacc tccaatggcc acagcgccgg cacctcgagc gtggcgccgt
     1141 cggcgatggc ctccagcatg gcggcggtct gtttcgtcgc ctgcgcgggg tgcacccacc
     1201 cgatcgccac cgaggcatcc gccacgaatg cccgtgtcaa ggtcgtccgt ggtcgatggc
     1261 cgcgcgcaca tcccgatcgg tgagcttggc cttggatcgt cgcccgatcc gctgctgcaa
     1321 gtcgcgcagc ccttcaacgc tccggcggac ctcgtcatgc cgctgtgcgc cctccggcac
     1381 gaggcgcgcg acggccttgt cgtggcgggt aatcacgacc tcttcgccct tcgagacgcg
     1441 atccaggagc tcgccgaagc gggtcttggc ctcgaacgcg gtgaccgtcg ccatcgctcc
     1501 tccagttatt gactagtcat tgactagttt atcacggcat acgtcgttga ccactgactg
     1561 gctaagatcc tcgccgtgcc ccgctcgtcg cccctcgatc tcttcgaccc cgtcgtccgc
     1621 gagtggttca cgcgttcgtt cgcgtcgccg acgacgccgc agaagctcgg gtggccggcg
     1681 atcgcgcggg gtgaatctac gctgatgctg gccccgaccg gcagcggcaa gacgctggcg
     1741 gcgtttctct ggtgcctcaa ccgcctgatg ttctccccgg ctccgccgcc gaaatctcgt
     1801 tgccgggtgc tgtacatttc gccgctcaag gcgctggcgg ttgacgtcga gcgcaacctg
     1861 cgcgcgccgc tggcgggaat cgccgcggtt gcccgggggg acgggaggga cgtcacctcg
     1921 cccgagattg cgattcgcac cggcgacacg ccggcgcccg accgcgcacg cttcgggcgc
     1981 gaggcggccg acatcctgat tacgacgccg gaatcgctgt tcctgctgtt gacctcgaac
     2041 gcgagggcgc gcctggcgac ggtggacacc gtcatcatcg acgagattca cgcgctggtc
     2101 ggcggcaagc gcggcgccca cctggcggtg tctctcgagc ggctcgaggc cattcgcccc
     2161 gccggagctc ccccgctaca gcggatcggg ctctccgcca cccagcgtcc gctcgaggaa
     2221 gtcgcccgtt ttctgggagg cgcggcaccg cccgcgcgca cgaagagacc tcgtgcccgg
     2281 aagcctgcag gacgccgatc ggcaccagcc accacagtgg aggccgagct ccgtgaaggg
     2341 ctggaaggca cgaacggcgc gatcgagtac cggcccgtca cgatcgtcga tgcggccgaa
     2401 aagaaaccgc ttgccctccg ggtcgaggta ccggtcgaag acatggcgcg tcctgttccc
     2461 gacgcaccaa aggacgcgcc cgtgccccag ggtcccgtgc atccctcgat ctggacggcg
     2521 gtccacccgc gcctgctgga tctgattcgc gcccaccggt ctacgctgct gttcgtcaac
     2581 agccgtcgtc tggccgagag gctggcagga gcgctgaacg acctggcggg cgagccgctc
     2641 gttcgctcgc aacacggttc gctgtctcac g
//
LOCUS       DBNGSH010000133         4157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1368131, whole genome shotgun sequence.
ACCESSION   DBNGSH010000133 DBNGSH010000000
VERSION     DBNGSH010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1368131"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /gene="queA"
                     /locus_tag="AC16CY_03055"
     CDS             <1..702
                     /gene="queA"
                     /locus_tag="AC16CY_03055"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR3352786.1"
                     /translation="RLYGEVLARHFHGRRTVRLWTDDGSGIAAAIDRIGHVPLPPYIT
                     RDDEASDRDRYQTVYARDPGSVAAPTAGLHFTTDMLERLRGRGIELATITLHVGYGTF
                     KPVRVELVEEHEVDQESFSVSPEAASALSCAMADGRRIIAVGTTTTRALESLNVETGG
                     RVNPVSGTTRLFIRPGHTFRVVQGLVTNFHLPRSSLLMLAAAFAGRERILAAYREAVE
                     RQYRFYSYGDAMLIL"
     gene            776..849
                     /locus_tag="AC16CY_03060"
     tRNA            776..849
                     /locus_tag="AC16CY_03060"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:810..812,aa:Met,seq:cat)
     gene            962..1957
                     /gene="tilS"
                     /locus_tag="AC16CY_03065"
     CDS             962..1957
                     /gene="tilS"
                     /locus_tag="AC16CY_03065"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR3352787.1"
                     /translation="MSHLHQQIRRTIRRHGLCPSGTRLLVGLSGGSDSVALLFLMKEL
                     APSGGFTLTSLAHLNHRLRASAARDEQFCRTLADEAGVPIVVESIDVAAYAQSQRLSV
                     EDAARRLRYDFLHRAAGTTGADRIAVGHTRDDQAETFLLKLIRGAGLTGLGGIYPRRE
                     KVVRPLLGVSRAELRAYLETRGQTWVEDETNAELENPRNRIRHRVLPELERAYGGDVS
                     HAIARAAAIVREDGQWLEELAGRRFDALVRRDEGRLEIDLKTLEAEPIPVRRRIVLNA
                     LRLAAAGREIGLDHVEAALEVAAGESAGVDVPGSRVELRAGKLVLVSRHPDRAPS"
     gene            2053..3948
                     /gene="ftsH"
                     /locus_tag="AC16CY_03070"
     CDS             2053..3948
                     /gene="ftsH"
                     /locus_tag="AC16CY_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3352788.1"
                     /translation="MRSLVFWMIMAVIAVMVWNFSNRLQTGPKVMSFTEFYDSIGKDQ
                     IAEVAMVGTEITGKTKAGETFRSSAPPQYDMLWNLLITNKIPVTYRDATVSPWTTVLG
                     TWAPILLMIGFWVFIMRQMQSGGNKALSFGKSKAKLSSSAQKKVTFKDVAGVDEAKEE
                     LQEIIDFLREPQKFQKLGGRIPKGVLLMGPPGTGKTLLARAVAGEANVPFFSISGSDF
                     VEMFVGVGASRVRDLFEQGKKNAPCIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQLL
                     VEMDGFESNEGVILVAATNRPDVLDPALLRPGRFDRRIVVNRPDVKGREGILAVHTKK
                     IPLSDDVDIAVLARGTSGFSGADLANLVNEAALNAARYNQKVVRMLDFEFAKDKVLMG
                     AERRSMIISEHEKRVTAIHEAGHALLTVKLPHADPIHKVTIIPRGMALGLTMQLPIDE
                     KHNYSREYLEDQIAILLGGRIAEEISIGSITTGAGNDLERATDLARRMVCEWGMSHSM
                     GPLTFGKKEEQIFLGREIAQHQDYSEDTALRIDQEVKRFVTDNYMRAQAILTENKQHL
                     LAMAEALLARETLDAEQVTRLASGLPLDELTPATDATPSQPRDAKPAKERPAIVPSLP
                     PRPVTQE"
     gene            3976..>4157
                     /locus_tag="AC16CY_03075"
     CDS             3976..>4157
                     /locus_tag="AC16CY_03075"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3352789.1"
                     /translation="MSPRRRFTLPLPGGRTLELGGRTLVMGIINVTPDSFSDGGRLLD
                     PARAVDAGVRMVEEGA"
BASE COUNT          735 a         1346 c         1372 g          704 t
ORIGIN      
        1 cggctctacg gagaagtcct ggcccggcac tttcatggcc ggcggaccgt ccggctgtgg
       61 accgatgacg gaagtgggat tgcggcggcg atcgatcgca ttggccacgt tccgctcccg
      121 ccctacatca cgcgggatga cgaggcgtcc gatcgcgatc gctatcagac cgtttacgcg
      181 cgcgacccgg gatcggtggc ggcgcccaca gcgggcctgc actttacgac ggacatgctc
      241 gaacggcttc gtggccgcgg catcgagctg gccacgatca cgcttcacgt cggctacggc
      301 acgttcaagc cggtacgtgt cgagcttgtc gaagaacacg aagtcgatca ggaaagcttc
      361 tcggtgtctc cggaagcggc gagcgctctt tcctgcgcga tggccgacgg tcgccggatc
      421 atcgccgtcg gcaccacgac gacgcgcgcg ctcgagtcgc tgaacgtcga aaccggaggc
      481 cgggtgaacc cggtcagcgg gacgacgcgt ctcttcatcc ggcccgggca cacgttccgc
      541 gtcgtccagg gactcgtgac caacttccac ctgccgcgct cgtcgctgct gatgctcgcg
      601 gccgccttcg ccggccggga acggattctt gccgcgtatc gcgaggccgt cgagcgccag
      661 taccggttct atagctacgg cgacgcgatg ctgatcctct aaggccccag ggcccagggc
      721 ccaggcctca ggcctcaggc ctcagaggcc cgaggcctgc taagatcgcc ctttggccgg
      781 agtagctcag tcggttagag cacgcgtctc ataagcgcgg ggtcggcggt tcgagtccgc
      841 cctccggcac taccaggcct cagggctcag gccgcaggcc tcagtggctt catcctgata
      901 ccctgccgtc tgatgccttg cccagccctg aggcctgagg cctgattcct ggggcctttc
      961 aatgtctcac ctccatcagc agatccgtcg aacgatccgc cgacacggcc tgtgtccttc
     1021 cggcacacgc ctgctcgtcg ggctctcggg cggatcggac tcggtcgcgt tgcttttcct
     1081 gatgaaagag ctcgccccat cgggcggctt cacgctgacg tcgcttgctc acttgaatca
     1141 tcgtctccgt gcctcggcgg ctcgcgacga acagttctgc cgtacgcttg ctgacgaagc
     1201 gggcgtcccg attgtcgtcg aatcgatcga cgtcgccgcg tatgcacaga gccagcgact
     1261 gtcggtagag gacgcggcgc gccggcttcg ttatgacttc ctccatcgcg ccgccgggac
     1321 gacgggcgcc gaccggattg cggtgggcca cacccgggac gatcaggccg agacgtttct
     1381 cctgaagctg attcgaggcg cggggctgac cggacttggc gggatctacc ctcgccggga
     1441 gaaggtcgtt cgacccctgc tcggcgtctc gcgcgccgag ctccgcgcgt atctggagac
     1501 gagggggcag acatgggtcg aggacgaaac aaacgccgag ttggagaacc cgcgtaatcg
     1561 catccggcac cgagtgctgc cggagctcga gcgtgcctac ggcggcgacg tgagtcacgc
     1621 gattgcacgg gcggctgcca ttgtccgaga agatggtcag tggctcgagg agctggccgg
     1681 ccgccgtttt gacgccctcg tgcgacggga cgagggccgg ctcgaaatcg acctgaaaac
     1741 gctcgaagcc gagccgattc cggtcaggcg ccggatcgtg ctgaatgccc tgcgtctggc
     1801 cgctgccggg cgtgaaatcg gcctcgatca cgttgaagcc gcactcgagg tggcagccgg
     1861 cgaatcggcc ggggttgacg ttccgggcag ccgtgtggaa cttcgggccg gaaaattggt
     1921 cttagtcagt aggcacccgg acagggcgcc gtcgtagatc agtggtaaca gcttcagggg
     1981 gtggccccga agtgatactc tgatagacag ttcggacgtt tcgggcccag ggaggctggt
     2041 ttgaactcga caatgcggag cctggtgttc tggatgatca tggccgtcat cgcggtcatg
     2101 gtgtggaact tttccaacag gcttcagacc ggcccgaagg tcatgagctt caccgagttc
     2161 tacgactcca tcggcaagga tcagatcgcc gaagtggcga tggtcggcac cgaaatcacc
     2221 gggaagacca aggcgggcga aacgttccgg agttccgccc cgccccagta cgacatgctc
     2281 tggaatctgc tgatcaccaa caagattcca gtcacctatc gcgacgccac ggtgagcccg
     2341 tggacgaccg tattgggcac gtgggccccg atcctcctga tgatcgggtt ctgggtgttc
     2401 atcatgcgcc agatgcagag cggcgggaac aaggcgctct ccttcggcaa gagcaaggca
     2461 aagctctcgt cgagcgcgca gaagaaggtg acgttcaagg acgtcgccgg cgtcgatgaa
     2521 gcgaaggagg agctgcagga gatcatcgat ttccttcgcg agccccagaa gttccagaag
     2581 ctcggtggac gcattccgaa gggcgtgctg ctgatgggcc cgcccggaac cggcaagacg
     2641 ctgctggcgc gggccgtggc cggggaagcc aacgttccgt tcttctcgat cagcggctcg
     2701 gatttcgtcg agatgttcgt cggcgtcggc gcatcccgcg tccgcgatct cttcgagcag
     2761 ggcaagaaga acgccccctg catcgtcttc atcgacgaga tcgacgcggt cggccggcac
     2821 cgcggcgcgg gcctcggtgg cggccacgac gaacgcgagc aaacgctgaa ccagctgctc
     2881 gtggagatgg acggcttcga gtccaacgaa ggcgtcatcc tggttgcggc gaccaaccgg
     2941 ccagacgtgc tcgatccggc gctgctgcgc cccgggcgct tcgaccgccg catcgtcgtc
     3001 aatcgtcccg acgtcaaggg ccgcgagggg attctggccg ttcacacgaa gaagatcccg
     3061 ctctccgacg acgtggacat cgccgtgctg gcgcgtggca cgtccggctt ctcgggcgcc
     3121 gacctcgcga acctggtgaa cgaagcggcg ctcaacgccg cgcgatacaa ccagaaggtc
     3181 gttcgcatgc tcgacttcga gttcgcgaag gacaaggtgc tgatgggcgc cgagcgccgc
     3241 tcgatgatca tcagcgagca cgagaagcgc gtgaccgcga ttcacgaggc gggccacgcc
     3301 ctgttgacgg tcaagctgcc gcacgccgat ccgatccaca aggtcacgat cattccgcgc
     3361 ggcatggccc ttggcctgac gatgcagctt ccaatcgacg agaagcacaa ctactcgcgc
     3421 gagtacctcg aggaccagat cgcgatcctg ctcggcggcc gcatcgccga ggagatctcg
     3481 atcggcagca tcacgaccgg ggccggcaac gacctcgagc gcgcgacgga cctggctcgc
     3541 cgcatggtct gcgagtgggg catgagtcat tcgatgggtc cgctcacctt cggcaagaag
     3601 gaagagcaga tcttcctcgg gcgcgagatt gcccagcacc aggactatag cgaggacacg
     3661 gcgctccgga tcgaccagga agtcaagcgc ttcgtgaccg acaactacat gcgcgcacaa
     3721 gccatcctca cggagaacaa gcagcacctg ctcgccatgg ccgaggcgct gctggctcgc
     3781 gagacgctgg atgccgagca ggtcacgcgc ctggcatcgg gcctgccgct cgacgagttg
     3841 acgccggcga ccgacgcgac gccgtcccag ccccgcgacg caaagccggc caaggaacgt
     3901 ccggcgatcg tgccgtcgct gcccccgagg ccggtgacac aggaataaga gtcaggaatc
     3961 cgctaagttt ctcccgtgtc tcctcgtcgt cgattcaccc tccccctgcc gggcggcagg
     4021 acgctggagc tgggtgggcg cacgctcgtc atggggatca tcaacgtcac tcccgactcc
     4081 ttctccgacg gggggcggct gctggatccg gcacgcgcgg tcgatgccgg cgtgcggatg
     4141 gtcgaggagg gggccga
//
LOCUS       DBNGSH010000134        14605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_179702, whole genome shotgun sequence.
ACCESSION   DBNGSH010000134 DBNGSH010000000
VERSION     DBNGSH010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 14605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14605
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_179702"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..81
                     /locus_tag="AC16CY_03080"
     tRNA            5..81
                     /locus_tag="AC16CY_03080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Val,seq:gac)
     gene            92..2059
                     /gene="thrS"
                     /locus_tag="AC16CY_03085"
     CDS             92..2059
                     /gene="thrS"
                     /locus_tag="AC16CY_03085"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR3352790.1"
                     /translation="MADVTITLPDGSIRSVPAGTTVADLATSISQGLANVALAAKVDG
                     RLVDLSFPLASDAAVQIVTSRNPADALPLYRHSTAHLLAAAVTNLFPTVQCGIGPPTD
                     EGFFYDFVVPRPFVPEDLEAIEQKMRELAAQDLPFERQLWPREQARRFFRERGEPLKV
                     QLIDEKTEGQSEVSCYTIKDKETFVDFCVGPHVPSTGRLKAFKLLSTSNAYWKGDARN
                     QPMQRVYGTAFFSEKELKEHLTRIEEAKKRDHRKIGREQGLFFFHPWAPGAPFWLAKG
                     TVLFNTLADYIRGVLFPAGYVEVKAPIVFNKALWETSGHWQHYRQNMFLVEGDEGEQM
                     ALKAMNCPGHMLIYASEKRSYRDLPIRYHEQTPLHRNEASGVLSGLTRVRQFSQDDAH
                     CFVMESQIGEEVEHVLRLVQRVYRDFGMKLELKLSTRPAEYLGTIEQWNHAEAELKRA
                     LEGVGEPYTVDEGDGAFYGPKIDFTVTDAILRRWQLATIQLDYQIPERFDLKYVGADN
                     AEHRPVVIHRAIFGSFERFIAMLIEHYAGAWPLWLAPVQAVVMPIVDRHTDYSAGVRD
                     RLARAGLRVELDDRQEKIGYKIREAQLQKIPYMLVVGDREVADGTLSVRSRSGGDLGA
                     RSVDQFIADALAEVGSKAAGSSGKQTDDGKP"
     gene            2082..2681
                     /gene="infC"
                     /locus_tag="AC16CY_03090"
     CDS             2082..2681
                     /gene="infC"
                     /locus_tag="AC16CY_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR3352791.1"
                     /translation="MAFDRSPRRDDRVRTNERIRVKEIRVIDDTGQQLGIMPPPQALV
                     IARQKGLDLVEISPTAVPPVCRIMDFGKYQYQEQKRSREAKKHQRVIEVKEIKFRPKV
                     DEHDYQFKKKHIERFLSDGDKVKATIFFRGREMAHPEIGRRILERLMGELGEVALAET
                     APRQEGNQMHIILSQRTGRKSSARPKADPGAESGPGADE"
     gene            2698..2895
                     /gene="rpmI"
                     /locus_tag="AC16CY_03095"
     CDS             2698..2895
                     /gene="rpmI"
                     /locus_tag="AC16CY_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR3352792.1"
                     /translation="MPKLKTHRGAAKRFKKTGTGKIVRASAFKRHILTGKTTKSKRQM
                     RGTREVAEQDAPKLRRMLPYK"
     gene            2909..3271
                     /gene="rplT"
                     /locus_tag="AC16CY_03100"
     CDS             2909..3271
                     /gene="rplT"
                     /locus_tag="AC16CY_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR3352793.1"
                     /translation="MPRVKRGTVRRAKRKKLLARAKGFYQTKSKLYRAAKESVDTALK
                     YAYVGRKNKKRDYRRLWVIRINAAARENGLTYSQLMRGLKAAGSALDRKMLADMAATA
                     PATFAKVAAQAKAALPAA"
     gene            3406..4473
                     /gene="pheS"
                     /locus_tag="AC16CY_03105"
     CDS             3406..4473
                     /gene="pheS"
                     /locus_tag="AC16CY_03105"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR3352794.1"
                     /translation="MEELRAAFESALAAASTEAALKSLNDAFLGRKSGKLTEQMKTLG
                     SLAPDARRELGGLLNALKAEIEGSIEAKRSVLSAARPLAGSVDITLPGRERRVGRVHP
                     LMQVRRQVEDIFTRMGYEILEGPEVEDDYHNFEALNMPPDHPARDTHDTLYLDTPIPG
                     ATWGAHRGGTPDAQVRAATLLRTHTSAMQIRYMKTFPPPVRIVVPGRVYRRDNLDLTH
                     TPMFQQFEGLVVGEHVTMADLKGTFEEMLRSLFGDVKVRLRPSFFPYTEPSAEVDISC
                     HKCGGSGCPICKQTGWLEILGSGMVHPALFEAVGYDPERVTGFAFGLGIERVAMLKYG
                     VEDIRLFYENDLRFLEQFPSS"
     gene            4484..6592
                     /gene="pheT"
                     /locus_tag="AC16CY_03110"
     CDS             4484..6592
                     /gene="pheT"
                     /locus_tag="AC16CY_03110"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR3352795.1"
                     /translation="MKILVSWLRDFVDVPGRAEDIAAMMSVRGFAVEGIEPFGDGDAV
                     IDFEVTGNRPDCMAVSGIAREVATAFGLQVRRPAVASTVRRTEPAGNGPALQLTALKT
                     VDKSDIEVFIDNQDLCSRYAGAVADVTLGPSPDWMQARLRAAGVRPTSNIVDVTNYVL
                     VELGQPMHAFDFDRIGGGRIRVRTAREGETLRTLDGKERTLAAEMLVIADAERAVAVA
                     GVMGGADSEVNDATRTIVLESAHFDPLSVRRTSRALGLKTEASLRFERGVDPRLPLTA
                     MERACALLEMIGAGTARGTVVDRHPVRAEPTVLRLRRGRAAGLIGTPVPDEDIRRILE
                     SLGFALRNADGGWDVTVPTRRVDVSREVDLIEEVARHYGFDKIPVTFPHVVAAPPPLD
                     PRIARARQLRGLMTGQGFSEAVTFGFTTAAAVAPFATDGSPVPIRNPLSEAFAVLRPS
                     LLPGLVESAGRNIRRGRRDVQLFEIGSRFMQPGGESETIAFVWTGAGRAQHWSERNRE
                     VDFFDAAAVVSTIADSFDADVDGAPAAPAFLVAGQSASMVARPREAGHTTAGTPVGVA
                     GRLAPDVGELLGLPAEVPAFVGELVLDTLSGFRRSSIKASAPPRLPSVDRDISILVSD
                     TMTARSVRDTVHGLAIPTLASLQEFDRYSGKGIPEGKISLSLRLTFRAPDRTLTDQEV
                     QKAMDSVLKALEDQHGAVQR"
     gene            6824..7099
                     /locus_tag="AC16CY_03115"
     CDS             6824..7099
                     /locus_tag="AC16CY_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352796.1"
                     /translation="MAKQAVATRTDLDPIDRLEEKVRTLVGLVTQLRAEHARSTEENF
                     RLSRELEHLRAHLAESEVSGNELDALRSERDVIRARVADMLRQLESI"
     gene            7129..7422
                     /locus_tag="AC16CY_03120"
     CDS             7129..7422
                     /locus_tag="AC16CY_03120"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR3352797.1"
                     /translation="MTVEIGGQRYPIRSALDEKYVTELAAYVDQKMRAASDAAPSSDL
                     LGLAVLVALNIADEFFRARDQRPSQGGGLQERAIRLERLVDEALAQAAVKRAV"
     gene            7475..7661
                     /gene="ssrS"
                     /locus_tag="AC16CY_03125"
     ncRNA           7475..7661
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16CY_03125"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7724..8728
                     /gene="thiL"
                     /locus_tag="AC16CY_03130"
     CDS             7724..8728
                     /gene="thiL"
                     /locus_tag="AC16CY_03130"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR3352798.1"
                     /translation="MNTSTVADCGERALVARIRERLTMPSWVVKGPGDDAAAIEPVRG
                     ALEVLTTDAQIDGVHFDCGFVPPDAIGHRALAVNLSDLAAMGARPRAALLSLALPGSL
                     ELASFDGILDGLLALARCHDVALVGGNITRTPGPLVVDVTAIGTVHRRKMLTRDGARA
                     GDDVYVTGTLGDAATGLRRLRAAQSSADAASEVEAARYLRPAPRVRAGMILGRNRAAS
                     SCMDLSDGLADGVRQIAEASGVGIVLDAEAIPIAPDVREWHGREGLDPLEAALSGGDD
                     LELLFTSRPNQAGRLRAVRRLVGDLPITRIGVVTRTTDLLVRDEKGSRQLPAGYEHFR
                     "
     gene            8769..9374
                     /locus_tag="AC16CY_03135"
     CDS             8769..9374
                     /locus_tag="AC16CY_03135"
                     /inference="COORDINATES: protein motif:HMM:NF018437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HIR3352799.1"
                     /translation="MPIGLLQSTRRQVVVLVIAVVGFVFMYETRMFDSRSALWQSMTQ
                     GRSPRPGSQLPFSATAYCKGTTTASGVGVRTGIAAADTAILPVGSVLNIAAGDSRYNG
                     VYTVMDTGPKIQGHRLDLYMWSCHEALSFGRKDVQVTVLRLGWDPRATSPTMIDRLFR
                     RRDARPIPPPDPPPPAGIAPEDPPVEVDSSSSAEPDQGEPQ"
     gene            9417..10244
                     /locus_tag="AC16CY_03140"
     CDS             9417..10244
                     /locus_tag="AC16CY_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352800.1"
                     /translation="MQIRVAAVVFCLLFVTARAEAQFQVPAAAPGEQFNVEVGLMFWL
                     PTPELRLQTDGLAAVGVPEVDFVQEFGLEREMFMEFRSVLKLGRKSKIRVSYLPIEYT
                     AQTTLQRDITFGGVTYPISIPVNMTYKLDTWRFGYEHDFVAADRGFLGLITELKRSQL
                     TGGLEAAGFGRQVTDVTAPIMALGGILRVYPHRNIAVTTEFTGFKVFGFVKDITEAIA
                     DDLDVEMWDFDLYGTFNFGRFVGAQVGYRRLSTNYLVDEDAGDMKFSGFYFGGLARF"
     gene            complement(10348..11085)
                     /locus_tag="AC16CY_03145"
     CDS             complement(10348..11085)
                     /locus_tag="AC16CY_03145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3352801.1"
                     /translation="MDVPLELTARAEATHFWFRGFRQFISPILLELTAAAQGVRIVDC
                     GCGVGHNMRLLQRYGRVVGFDRNQTGVRLTRASGATVVRADAAHAPFRSEAFDIATAF
                     DVLPFVESDVAAVREMARMVRRGGSVVLTMAALEVLRGDHSESWGEIRRYTPAMARRV
                     LEQADLRVERVSFLFGSLFPLMLSVRFVQRLLRPFRGPRHDTDIAVPWAPVNALLTGI
                     VTVEARAARFVRLPFGSSLLAVGRKLV"
     gene            complement(11085..13277)
                     /locus_tag="AC16CY_03150"
     CDS             complement(11085..13277)
                     /locus_tag="AC16CY_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3352802.1"
                     /translation="MRWLATAAFALATGLVALGQTVPAVPAAQRTLSATEHPPLPRDL
                     SEYWFVPASARVRPRPDEARFVQGVRAIAAGDFAKGLALIDGSNLDETALSDYVTFYR
                     AIALQGASRLAEADAALTSLVERKPQGYLNEAAALKLAEAVLARGNAQRAERILRALP
                     VSRLGEPEDTLLALGETEEAAEHRDHALDAYRRVYYGFPASEQAVEAKAAIDRLQAAG
                     NVPPASFGDALSRAERLFSARRWADARAAFEPLIAAASGDDKELATIRVAECDYRLGR
                     HRAARDQLKPFLDGGSREAEARYFYLNAVRGLGDRAASVALSRTLVTDHPTSEWAAEA
                     LNDLASRYLVDDQDAAADRVFRELLLRFPRHRYSERAAWKVGWTAYRSQRFAEAATVF
                     ESAAMTFPRADYRPSWLYWSGRARAEAGDKTGASARYRLVVADYGSSYYGRLASKLGG
                     GSMPAVVPARAQAAAAGSSVAPPPSVHLIRALIAAGLFDDALREVQYAQRTWGDSSQL
                     QATFAWIRNQQGRDLRADERFIAMRGAITTMRRAYPQFLSAEGDTLPVEVLGIIFPLD
                     YWDLIQKYSSAHGIDPYLITALMAQESTFTAEIRSSANARGLMQVMPSTGRVYARKLG
                     IRPFSTGSLSQPETNVRIGTRYFKDLMDRFGAHHYALAAYNAGENRVAQWLKEAPGLP
                     PDEFIDNIPFPETQNYVKRILGTVEDYRRLYATGRLTAAAGSTTTATN"
     gene            complement(13274..14314)
                     /locus_tag="AC16CY_03155"
     CDS             complement(13274..14314)
                     /locus_tag="AC16CY_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352803.1"
                     /translation="MSSLDEQVRSALDRVVESLKGQLESDLTTSAADILRAVAEDRDR
                     ADADRRQETDQQLAALRQEFERERAEFQRQTADEIAALKQTIESVRSELGTAALATEE
                     ERQQREAVQSRLEDSRREAEDARRELDDIRRVMEAAQQETQKARVDAGDTRRELDSVR
                     TALLRADSLVSGLRALNGAQSLGDVLEALFDLASRESGRAAVFLLRGGGLVAWRARGF
                     DTTVLRVGADLVPGQAGLVAAAAQAGARKDHPEDGGTGLPAFAVGNGPCHAVALPVEV
                     SGSVIAVLYADKPEADNQEHARWPTILDQMARHAGRVLEAVTVRHAAAMWAPRAAANV
                     PSTVQPTPREAV"
BASE COUNT         2550 a         4849 c         4916 g         2290 t
ORIGIN      
        1 atataggcgc gtagctcagt tggttagagc gcctccctga cacggaggag gtcagtggtt
       61 cgagtccacc cgcgcctacc aacacttctt gatggccgac gtcacgatca cattgcccga
      121 cggatcgatc cgaagtgtcc ctgcggggac cacagtcgcc gatctcgcga cgtcgatctc
      181 acagggcctg gcgaacgtcg cgctggccgc caaggtcgac ggccggctcg tcgatctgtc
      241 gtttcccctg gcgtcggacg cggcggtcca gatcgtcacg tcgaggaatc cagctgacgc
      301 gcttcccctc tatcgtcaca gcaccgcgca cctgctggcg gcggcggtga ccaacctgtt
      361 tccaacggtg cagtgcggca tcggcccgcc gaccgacgaa gggttcttct acgacttcgt
      421 ggtgccgcgc ccgttcgtgc cggaggatct cgaggccatc gagcagaaga tgcgcgagct
      481 ggcggcccag gatcttccct tcgagcgcca gctctggccg cgcgaacagg ccaggcgctt
      541 cttcagggag cgtggcgagc cgctgaaggt gcagctcatc gacgagaaga cggagggcca
      601 gagcgaggtc tcctgctaca cgatcaagga caaggagacc ttcgtggact tctgcgtcgg
      661 tccccacgtg ccgtccaccg gacgactgaa ggccttcaag ctgctgagca cgtcgaacgc
      721 gtactggaaa ggcgacgcgc gcaatcagcc gatgcagcgc gtgtacggca cagcgttctt
      781 ctcggagaag gagctgaaag agcacctgac ccggatcgag gaggccaaga agcgcgacca
      841 ccggaagatc gggcgcgagc aggggctctt cttctttcat ccgtgggcgc cgggcgcccc
      901 tttctggctc gcgaagggga ccgtcctctt caacacgctg gccgactaca tcaggggcgt
      961 gctgttcccg gcggggtacg tcgaggtcaa ggctccgatc gtcttcaaca aggcgctctg
     1021 ggagacgtcc gggcactggc agcactaccg gcagaacatg ttcctcgtcg aaggcgacga
     1081 gggcgagcag atggccctca aggcgatgaa ctgcccgggc cacatgctga tctacgcgag
     1141 cgagaagcgc agctacaggg acctgccaat ccgttatcac gagcagacgc cgctccaccg
     1201 caacgaggcg tcgggtgtcc tgtccggcct gacacgggtc cgccagttct cgcaagacga
     1261 tgcgcactgc ttcgtgatgg agtcgcagat cggcgaggag gtcgagcacg tgctgcggct
     1321 ggtgcagcgc gtgtatcgcg acttcgggat gaagctggag ctcaagctgt ccacgcgccc
     1381 ggcggagtac ctcgggacga tcgagcagtg gaaccacgca gaagccgagc tcaagcgcgc
     1441 gctcgaaggc gtcggcgagc cgtacaccgt cgacgaaggc gatggcgcct tctacggacc
     1501 gaaaatcgac ttcacggtca ccgacgcgat cctccggcgg tggcagctgg cgacgattca
     1561 gctcgactat cagattccgg agcgcttcga cctcaagtac gtgggcgccg acaacgccga
     1621 gcaccggccc gtggtcatcc accgcgccat tttcggcagc ttcgagcgat tcatcgccat
     1681 gctcatcgag cactacgcag gggcctggcc gctgtggctg gcgccggtcc aggcggtcgt
     1741 catgccgatc gttgaccggc acacggacta ctcggctggt gtccgcgacc ggctggcgcg
     1801 ggccgggctg agggtggagc tggacgaccg tcaggagaag atcggttata agatccggga
     1861 agcccagctg cagaagattc cctacatgct ggtcgtcggc gaccgggagg tggccgatgg
     1921 tacgctgtcg gtgcgaagcc gctcgggtgg cgatctcggt gcgcggtccg tggaccagtt
     1981 catcgccgac gccctcgcgg aagtcggtag caaggcagcc ggcagcagtg gtaaacagac
     2041 tgacgacggt aaaccgtaga ccgtagacct cggaggacga tatcgcattc gatcgctcgc
     2101 ccagacgtga tgaccgcgtt cgcaccaacg agcgcatccg ggttaaagaa atccgggtga
     2161 tcgacgacac cggtcagcag ctcggaatca tgccgcctcc gcaggcgctc gtcatcgcgc
     2221 gtcagaaggg actcgacctg gtcgagatct cccccaccgc ggtgccaccc gtctgccgga
     2281 tcatggactt cgggaagtac cagtaccagg aacagaaacg gtcccgcgag gccaagaagc
     2341 accagcgggt catcgaggtc aaggagatca agttccggcc gaaggtggac gagcacgatt
     2401 accagttcaa gaagaagcac atcgagcgct tcctcagcga cggggacaag gtcaaggcca
     2461 cgatcttctt ccgcgggcga gagatggcgc acccggagat cgggcgcagg attctcgagc
     2521 gcctgatggg cgagctcggc gaggtcgcgc tcgccgaaac cgcgccgcgc caggagggca
     2581 atcagatgca catcatcctg agccagcgca cggggcggaa gagcagcgcg cggccaaagg
     2641 ccgatccggg cgcagagtcc ggacctggag ccgacgagta ggaagagtgg accgacgatg
     2701 ccaaagctga agacacatcg gggcgccgcc aagcgcttca agaagaccgg aacgggcaag
     2761 atcgtcaggg ccagcgcctt caagcgccac atcctgacgg gcaagacgac caagagcaag
     2821 cgccagatgc ggggcacacg ggaggtggcc gagcaggacg cgccgaagct gagaaggatg
     2881 ctcccgtaca agtgaggaat tcaccgcaat gccgagagta aagagaggaa cggtccggcg
     2941 agccaagcgc aagaagcttc ttgcccgggc caagggtttc taccagacca agagcaagct
     3001 gtatcgcgcc gccaaggaat cggtagacac ggcgctgaag tacgcctacg tcgggcgcaa
     3061 gaacaagaag cgcgactacc ggcgtctgtg ggtcatccgc atcaatgccg cggcgcggga
     3121 gaacgggctg acctactcgc agctgatgcg cggcctcaag gccgccggca gcgcgctcga
     3181 ccgcaagatg ctcgccgaca tggcggcaac ggcgcccgcg accttcgcca aggtcgctgc
     3241 gcaggccaag gcagctctgc ccgcggcata accccggacc tcgcttgtgt cggtgatggc
     3301 accaaggctc gtcgtgactg caaccggccg tgttgtcagc ccgccactgc ccggagcccc
     3361 cccacacaag cgagctgcag cgtagtgatg gccgatcgcg gggccattga ggaactccgg
     3421 gcggccttcg agtccgccct cgccgcagcg agcaccgagg cggcgctcaa gagcctgaac
     3481 gacgcattcc tgggccgcaa gtcgggaaag ctgaccgagc agatgaagac gctcggttcg
     3541 cttgcccccg acgcccgacg tgagctcggc ggcctgctga acgccctcaa ggccgagatc
     3601 gaaggatcga tcgaagccaa gcgctcggtc ctgtccgcgg cgcgccccct ggcggggtcc
     3661 gttgacatca ccctcccggg gcgcgagcgc cgcgtcgggc gcgttcaccc gctcatgcag
     3721 gtgcgacggc aggtcgagga catcttcacc cgcatgggct acgagatcct cgagggcccg
     3781 gaggtcgagg acgactacca caacttcgaa gcgctcaaca tgccgccgga ccatccggcg
     3841 cgcgacacgc acgacacgct ctacctcgac acgccgattc ccggcgccac gtggggcgcg
     3901 cacagaggtg ggactccgga cgcccaggtt cgcgcggcca cgctgcttcg cacgcacacg
     3961 tccgcgatgc agatccgcta catgaagacg ttccccccgc cggtgcgcat cgtcgtgccc
     4021 gggcgcgtct atcgccgcga caacctggac ctgacgcaca cgccgatgtt ccagcagttc
     4081 gaggggctcg tcgtgggcga gcacgtcacg atggcggacc tcaagggcac cttcgaggag
     4141 atgctcaggt cgctcttcgg cgacgtgaag gtccgtcttc gcccgagctt ctttccctac
     4201 acggagccga gcgccgaggt cgatatcagc tgccacaagt gcggtggatc cggctgcccg
     4261 atctgcaagc agaccggatg gctggagatc ctgggcagcg gcatggtcca tccggcgctc
     4321 ttcgaagccg tggggtacga ccccgagcgg gtgaccggct tcgccttcgg cctcgggatc
     4381 gagcgcgtgg ccatgctcaa gtacggggtc gaggatattc gcttgttcta cgagaacgat
     4441 ctccggttcc tcgagcagtt tccttcgtcg tgatggacgc ctcgtgaaga tcctcgtttc
     4501 gtggcttcgt gacttcgtgg acgtgcccgg ccgcgcggaa gacatcgcgg ccatgatgtc
     4561 ggtgcgcggc ttcgcggtcg aaggcatcga gccgttcggc gacggtgacg ccgtgatcga
     4621 tttcgaagtg acgggcaacc ggcccgattg catggccgtg agcgggatcg cccgcgaggt
     4681 ggccacggcg ttcgggctgc aggttcgccg gcccgccgtc gcctcgaccg tgcgtcgcac
     4741 ggagccagcc gggaacggac ccgcgctgca gttgacggcg ctcaagacgg tggacaagag
     4801 cgacatcgaa gtcttcatcg acaaccagga tctctgctcg cgatacgccg gcgccgtggc
     4861 ggacgtcacg ctcggaccat ctccagactg gatgcaggcc cgcctgcgcg cggcgggagt
     4921 ccggcccacg agcaacatcg tggacgtgac caactacgtg ctcgtggagc tcggtcagcc
     4981 gatgcacgcc ttcgacttcg atcggatcgg cggcggccgg atccgcgtga ggacggcgcg
     5041 ggagggagaa acgctgcgaa cgctcgacgg caaggagcgg acccttgccg ccgagatgct
     5101 cgtcattgcc gatgccgaac gggcagtggc ggtcgccggc gtgatgggcg gcgccgactc
     5161 ggaagtcaac gacgccacac gcaccatcgt gctcgagagc gcgcacttcg acccgctgtc
     5221 ggtgcgccgg acgagccggg cactcgggct gaagaccgag gcgagcctgc gcttcgagcg
     5281 cggcgtcgat ccccggcttc cgctgaccgc catggagcgg gcctgcgcgc tgctcgagat
     5341 gatcggcgcg ggtacggcgc gcgggacggt cgtcgatcgc cacccggtgc gcgccgagcc
     5401 gacggtgctg cgactccggc gggggcgcgc ggcgggcctc atcggcactc cggtgccgga
     5461 cgaggacatc cggcggatcc tggagagcct cggcttcgcg ctccgaaacg ccgatggcgg
     5521 ctgggacgtc accgtgccga cacggcgcgt ggacgtgtcg cgcgaagtgg acctcatcga
     5581 agaggtcgcg cgccactacg gattcgacaa gatcccggtg acgtttccgc atgtcgtcgc
     5641 ggcgccgccg ccgctcgacc cgcggatcgc gcgcgcccgc cagctccgcg ggctgatgac
     5701 gggtcagggc ttttccgagg ccgtgacatt cggcttcacg acggcggccg ccgtcgctcc
     5761 gttcgccacc gacgggtctc cggtgccgat ccggaacccc ctctccgagg cgttcgcggt
     5821 gctgcggccg tcgctgctgc ccggcctcgt cgaatcggcc gggcgaaaca tccggcgggg
     5881 ccgccgcgac gtgcagttgt tcgagatcgg cagccgcttc atgcagccgg gcggcgagag
     5941 cgagacgatc gcgttcgtct ggacgggcgc cgggcgcgcc cagcactgga gcgagcggaa
     6001 tcgcgaggtc gacttcttcg atgcggcggc cgtcgtctcg acgatcgccg acagcttcga
     6061 tgccgacgtc gacggggcgc cggccgcgcc agccttcctg gtcgccggcc aatcggcgtc
     6121 gatggtggcg cgacccaggg aagccgggca cacgacggca ggaactccgg tcggcgtcgc
     6181 cggccgcctc gcgccggacg tgggcgagct tctcggcctg ccggccgagg tgcctgcctt
     6241 cgtcggcgag ctcgttctgg atacgctttc ggggttcagg cgaagctcga tcaaggcatc
     6301 tgccccacct cgactcccgt cagtggaccg ggacatttcc atcctggtca gcgacacgat
     6361 gacggctcgc tcggtacgcg acacggtgca cgggctggcc atcccgactc tcgcgtcgct
     6421 gcaggagttc gatcgctact cgggcaaagg gatccccgag ggaaagatca gcctgtcgct
     6481 gcgcctcacg ttccgcgcgc ccgaccggac gctcacggat caggaggtgc aaaaggcgat
     6541 ggactcggtc ttgaaggcgc tcgaggatca acacggcgcc gtacagcgct gactagtgga
     6601 ctgtatcaac tggaagagta cgggaacaac gagcttcgac agcgattctc cggccgcgac
     6661 aacgcccccc gacttcgctc ggggcgtcct gagcgatagt cgaaggacgg agcgccggta
     6721 gtattccgcg aaggacatac cactaggtgc ttgccagcgc cgtcagttcg tggcacgatc
     6781 acccgcgaac tcccctcctt cagacctacg aggatcgcac cggatggcca agcaggcggt
     6841 agcaacgcga accgatctcg atcccatcga tcggctcgaa gaaaaagtca ggacgctcgt
     6901 cggtctcgtg actcagcttc gggcggagca cgcacggtcg actgaagaga acttccgtct
     6961 ctcgcgcgag ctcgagcacc tgcgcgcgca cctcgccgag agcgaggtca gcggcaacga
     7021 gctcgacgcg ctcagatcgg agcgggacgt gatccgcgcg cgcgtggccg acatgctgcg
     7081 gcagctggaa tcgatctgac gcccagcctg ccgacgatga gcagcgtcgt gactgttgag
     7141 atcgggggcc agcgttaccc catacgcagc gcgctcgatg aaaagtacgt caccgagctg
     7201 gccgcgtacg tggaccagaa gatgcgtgcg gcctccgacg ccgcgccctc gagcgatctg
     7261 ctcggcctgg ccgtcctcgt agccctcaac atcgccgacg agttcttccg cgcgcgggat
     7321 cagcgcccgt ctcagggcgg gggcctgcag gaacgtgcaa tccggctcga gcgcctcgtg
     7381 gacgaagcgc tggcgcaggc cgcagtcaag cgcgccgtct aaccccggaa ctccactagc
     7441 cttgacccac ccagggtcga acagtaagat cgagacgctc ctgctttgct cgtgatggtt
     7501 tcggaggctc gtctgagcca acgtaacaac ccaagcgagc cgcgaggttg cgcggtgtgc
     7561 atgcctctgt gccgaggaag cctgaagcgc ggccgataac agcggctcca cctgctttcg
     7621 caggttcatt cgacctcccc acacggcaaa gcggggctct tcgtatctcc ctgatcgccg
     7681 ctcggccgga tcgagcggac gccctgatcg caccgacgag accttgaaca cgtccactgt
     7741 cgccgactgc ggagagcgcg cgctcgtcgc ccgcatccgg gaacgcctca cgatgccgtc
     7801 ctgggtggtg aagggccccg gtgacgacgc cgccgcgatc gagccggtgc ggggcgccct
     7861 cgaggtgctg acgaccgacg cccagatcga cggcgtgcat ttcgattgcg ggttcgttcc
     7921 gcccgatgcg atcgggcatc gcgccctggc ggtgaacctg agcgatctgg ccgcgatggg
     7981 agccaggccg agagccgcgt tgttgtcgct ggcgctcccc ggctcgctcg agctggcctc
     8041 gttcgacggg atactcgatg gactgctcgc gctcgcccgc tgtcacgacg tcgcgctcgt
     8101 cggcggcaac atcacacgca cgccgggccc gctcgtcgtc gacgtgacgg caatcggcac
     8161 cgtgcaccgg cgtaagatgc tgacgcgcga cggcgccaga gcgggcgacg acgtctacgt
     8221 gacggggacc ctcggcgacg ccgcaaccgg gcttcgaagg cttcgcgcgg cccagtcgag
     8281 cgccgatgcc gcgagcgaag tcgaggcggc ccggtatctg cgcccggcgc cccgcgtgcg
     8341 cgccggcatg atcctcgggc gaaaccgggc agcctccagc tgcatggatc tcagcgacgg
     8401 actggccgac ggcgtgcgcc agatcgccga ggcctccggc gtcggcatcg tgctcgacgc
     8461 cgaggcgatt cccattgccc cggacgtgcg cgaatggcac gggcgcgagg gccttgaccc
     8521 gctcgaagcg gccctgtccg gaggcgacga tctggagctc ctgttcacgt cccggccgaa
     8581 ccaggccggc cgacttcggg ccgtacggcg gctggtgggc gatctgccga taacccgaat
     8641 cggggtggtc accaggacca ccgacctgct ggtgcgcgac gagaaaggct cgcggcagct
     8701 tccggcgggg tacgaacact ttcggtaagc acggagacgc gcgctcctga cgaggtgcgc
     8761 gaacacggat gccgatcggt cttttgcagt ccacgaggcg tcaggtcgtc gtgctggtga
     8821 tcgccgtggt cggcttcgtg ttcatgtacg aaacccggat gttcgactcg cgcagcgcgc
     8881 tctggcagtc gatgacgcag ggccggtcgc cgcggcccgg atcgcagctg ccgttcagcg
     8941 cgacggcgta ctgcaaggga acgacgaccg cctcgggtgt gggcgtgcgc acggggattg
     9001 cggcggccga cactgcgatt ctcccggtcg gcagcgtgct caacatcgcc gccggcgaca
     9061 gccgctacaa cggggtctac acggtgatgg acaccggacc gaagattcag gggcacaggc
     9121 tcgatctgta catgtggagc tgccacgagg cgctgagctt cgggcggaaa gacgtgcagg
     9181 tcaccgtgct gcggctcgga tgggatccac gggccacgtc gccgacgatg atcgaccgcc
     9241 tgttcagacg gcgcgacgcg cgcccgattc ctccgccgga cccgccgccg cctgccggca
     9301 tcgcacccga agacccgccg gttgaggtcg attcgagctc gtccgctgaa cccgatcagg
     9361 gcgagccaca gtagcctcgc ctaaaccccc aagccgcgcg cgccgataga cgaggcatgc
     9421 aaatccgcgt tgcggccgtc gtcttctgcc tcctgttcgt taccgcccgg gccgaggccc
     9481 agtttcaggt gcccgcggcc gctccaggcg agcagttcaa tgtcgaggtc gggctgatgt
     9541 tctggttgcc aacccccgag ctccggctgc agacggacgg actggccgca gtgggcgtgc
     9601 ccgaagtgga cttcgtccag gagttcgggc tcgaacggga aatgttcatg gagttcagga
     9661 gcgtcctcaa gctcggacgc aagagcaaga tccgcgtctc ctacctcccg atcgaataca
     9721 ccgcgcaaac gacgctgcag cgcgacatca cctttggcgg agtcacttat cccatctcga
     9781 tccccgtcaa catgacgtac aagctggaca cgtggcggtt cggctacgag cacgacttcg
     9841 tggcggccga ccggggtttc ctcggactca tcaccgagct caaacgctcg cagctgacgg
     9901 gcggcctcga agccgcgggc ttcggaaggc aggtgaccga cgtcacggcg ccgatcatgg
     9961 cgcttggagg catcctccgc gtgtacccgc accgcaacat cgcggtcacc accgagttca
    10021 cgggcttcaa ggtctttggc ttcgtcaagg acatcactga agccatcgcc gacgatctcg
    10081 acgtcgagat gtgggacttc gatctctacg gcaccttcaa cttcggacgc ttcgtcggcg
    10141 cgcaggttgg ctaccggcgc ctctcgacga actacctcgt ggacgaagat gccggtgaca
    10201 tgaagttcag cgggttctac ttcgggggcc tcgcccgctt ctagtggacc gtatcaactg
    10261 gaagagtacc gcgaccaacg agctttgaca gcggttttcc ggccgcgaca acgaagcggc
    10321 cggcagttat cctcgaacga cataccacta aacgagcttt cttccaaccg ccagcagcga
    10381 gctgccaaaa ggcaggcgca cgaatcgcgc cgctctcgct tccacggtca cgatccccgt
    10441 caagagtgcg ttcaccggcg cccagggaac cgcgatgtcg gtgtcgtgtc tcggcccgcg
    10501 gaaggggcgc agcagccgct gtacgaacct gacgctcagc atcagcggaa acagggatcc
    10561 gaagagaaac gagacgcgct ccacccggag atcggcttgc tcgagcaccc gcctggccat
    10621 tgccggcgtg tatcggcgaa tctcccccca ggactcggaa tggtctccgc ggagaacctc
    10681 gagcgcggcc atcgtcagaa cgacagatcc gcccctgcgg accatccgcg ccatttcacg
    10741 aactgcggcg acgtcggact cgacgaacgg gagcacgtcg aacgcggtgg cgatgtcgaa
    10801 cgcctccgaa cggaacgggg catgtgcggc gtcggccctg acgacggtcg ccccggacgc
    10861 gcgcgttaac cttacgcccg tctggttcct gtcgaagccg acgacgcgcc cgtagcgctg
    10921 gagcagccgc atgttgtgcc cgactccgca gccgcagtcg acaatgcgca ctccctgggc
    10981 cgcagcagtg agctcgagca ggatcggtga gatgaactgg cgaaagccgc ggaaccagaa
    11041 gtgggtggct tcggcgcggg ctgtcagctc cagcggtacg tccatcaatt cgtggccgta
    11101 gtcgtcgaac ctgcggccgc ggtgagccgg cctgtcgcat acagacgtcg gtagtcctcg
    11161 acggtcccga ggattcgctt cacgtagttc tgcgtttccg gaaacgggat gttgtcgatg
    11221 aactcgtcgg gcggcaggcc cggggcttcc ttgagccact gagccacgcg attttctccg
    11281 gcgttgtagg cagccagggc gtagtggtgc gccccgaacc gatccatcag gtccttgaag
    11341 taccgggtgc cgatcctgac gttggtctcc ggctgcgaca gcgatccggt cgagaagggg
    11401 cggatgccga gcttgcgcgc gtagacgcgc cccgtcgacg gcatgacctg catcaggccg
    11461 cgcgcgttgg ccgacgaacg aatctcggcg gtaaaggtcg attcctgcgc catcagcgcc
    11521 gtaatcagat acggatcgat tccatgggcg gaggagtact tctgaatcag gtcccagtaa
    11581 tcgagcggga agatgatgcc gagcacctcc accggtagcg tgtcgccctc cgccgataga
    11641 aactgcgggt atgcccggcg catcgtcgtg attgcgcctc gcatcgcgat gaaccgttcg
    11701 tcggcccgaa ggtcgcggcc ctgctggttg cgaatccatg cgaacgtggc ttgaagctgc
    11761 gatgaatcgc cccaggtccg ttgagcgtac tgaacctccc tgagggcgtc gtcgaagaga
    11821 cccgccgcga tgagggctcg tatcaggtgg accgacgggg gtggcgcgac cgaagatccg
    11881 gcagcggcgg cctgggcgcg cgccggcact accgccggca tcgacccacc accaagcttc
    11941 gacgcaagcc gtccatagta cgagctgcca tagtcggcta ccaccagccg gtatcgcgcc
    12001 gatgcccccg tcttgtctcc cgcctcggca cgggctcgtc cagaccagta caaccacgag
    12061 ggtcgatagt cggccctcgg aaacgtcatg gcggccgact cgaagaccgt ggccgcctcg
    12121 gcgaaccgct ggctgcgata cgcggtccac ccaaccttcc acgccgcgcg ctccgagtag
    12181 cgatgcctgg ggaatcggag gagcagctcg cggaagacac gatcggcggc ggcgtcctgg
    12241 tcgtcgacga ggtaccgcga ggcgagatcg ttcagggcct cggctgccca ttcgctggtt
    12301 ggatggtcgg tgaccagcgt gcgggacaac gcgacggagg ccgcgcgatc gccgaggccg
    12361 cgcacggcgt tcagatagaa gtagcgcgct tcggcttctc tcgacccgcc gtcgaggaac
    12421 ggcttgagct gatcgcgggc cgcacggtgg cggccgagac gatagtcgca ctcggccacc
    12481 cggatggtcg ccagctcctt gtcgtctcca gacgccgccg cgatcagcgg ctcgaaggcg
    12541 gcccgcgcat cggcccagcg tctcgcgctg aacaaccgct cggcccgtga cagcgcgtcg
    12601 ccgaacgacg ccggaggcac gttcccggcc gcttgcagcc ggtcgatggc ggctttcgcc
    12661 tcgacggcct gctcgctcgc cgggaagccg tagtacacgc ggcggtacgc atcgagagcg
    12721 tggtcgcggt gttccgccgc ttcctcggtt tctccgagcg ctaggagcgt atcttccggc
    12781 tcgcccagtc tcgacacagg cagcgcgcgg aggatgcgct cggcacgctg cgcattcccg
    12841 cgagcgagaa cggcctccgc cagcttgagc gccgccgcct cgttcaagta cccctgcggt
    12901 ttgcgctcga cgagcgaggt caacgccgcg tcggcctccg ccaggcgcga cgccccctgc
    12961 agcgcgatcg cccggtagaa ggtcacgtag tcggacagcg ccgtctcatc gagattcgaa
    13021 ccgtcgatga gagcgagccc cttggcgaaa tcgcccgccg cgatcgccct gactccctgc
    13081 acgaagcgcg cttcgtcggg gcggggccgc accctcgctg acgcgggcac gaaccagtac
    13141 tcggagagat cgcgaggaag cggcgggtgc tcggttgcgg agagcgttcg ctgcgccgcc
    13201 ggaaccgccg gcacggtctg ccccagcgcg accaggcccg tcgcaagcgc gaacgcagcc
    13261 gtggcaagcc acctcacacg gcctcccgag gggtcggctg aacggtggat ggaacgttcg
    13321 cggccgcccg gggcgcccac attgccgcgg cgtgcctgac ggtgacggcc tcgagcaccc
    13381 gcccggcgtg tctggccatc tggtcgagaa tcgtgggcca tcgcgcgtgt tcctgattat
    13441 cggcctccgg cttgtcggca tacagcaccg cgatcaccga tccgctgacc tccacgggaa
    13501 gggcgacggc gtgacaaggg ccgttgccta cagcgaacgc cggcagtccg gtgccgccat
    13561 cctcggggtg atccttccgg gcgcctgcct gcgcggcggc ggcgacaaga cctgcctgcc
    13621 ctggcaccag gtcggcgccg acgcgcagca cggtcgtgtc gaaccctcgc gcgcgccagg
    13681 cgacgagccc gccgccgcga agaaggaaga cggccgcgcg gcctgattcc cgcgaggcga
    13741 gatcgaagag agcctcgaga acgtcaccga gcgactgggc gccgttcaac gcgcgcaaac
    13801 cggagacaag actgtcggcg cgcaggagcg cggtccgtac agagtcgagc tctcgacgcg
    13861 tgtcgccggc gtctacgcgc gctttctgcg tctcctgttg ggcggcctcc atcacgcggc
    13921 ggatgtcgtc gagctcgcgc cgggcatctt cggcttcgcg ccgactgtcc tcgagccggc
    13981 tctggacggc ttcacgctgc tggcgttcct cttctgtcgc gagggcggcg gtgccaagct
    14041 ctgaacgaac cgactcgatc gtctgtttca gcgcggcgat ttcatccgcg gtctggcgct
    14101 gaaactcggc ccgttcacgt tcgaattcct gacgaagcgc tgcgagttgt tgatccgttt
    14161 cctgccgccg atcggcgtct gcccgatcgc gatcctcagc cacggcgcgc agaatgtcag
    14221 cggctgacgt cgtgaggtcg gactcgagct ggcccttgag ggattcgacg acgcggtcga
    14281 gagccgatct gacttgttcg tcaagcgagg acacagttgt gctgattata agggtgcgag
    14341 gggacgaagg gacgaaggga cgaaggtacg aaggtgcgag ggtgcgaagg tgcgaaggtg
    14401 cgagggtgcg gggcgcttgc cgcgccaggg ggaataggac gagctagcgg aagatctggt
    14461 tcagccgtgc ttcgctgacg aaacgtgtct gccggacttt ccactggccg ccggacagca
    14521 cccacacttg agagaccgga gacactctgg gtgctggcag cgtcgccgac tgctccgtca
    14581 tgacggcggt caataccgcc gtctc
//
LOCUS       DBNGSH010000135        18598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4594886, whole genome shotgun sequence.
ACCESSION   DBNGSH010000135 DBNGSH010000000
VERSION     DBNGSH010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 18598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18598
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4594886"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2559)
                     /locus_tag="AC16CY_03160"
     CDS             complement(<1..2559)
                     /locus_tag="AC16CY_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352804.1"
                     /translation="MASVIQHLTDVSTGYKVFEDNQVLTKEPLNGLVDYLDDHQRLTR
                     VALMGVGIVAGLRLSVSNNVVVLTKGIGVTTDGDLIRVPRDLEYTRFRTYDETAPRYD
                     PFYDGQSMVVKVFELVPEEVDTAATLDTFSTATGKKLDEMVALLLMESFLKDQDLCTG
                     GDCDNKGKDALGNVRLLVLDQNDIGPLISKIATLDTLSQAFSPLFADRVALSAQTGSA
                     AALNQAYRTAANATRDRLKQALQGQVVSAAGMLGEVFTGNPAPGWIAKLDTAVPDTGI
                     QAYYDFLSDLVDAYNALGESLAGETSLLCPALDLFPKHLLLGVVGVPSPPPLLRTGFY
                     PSPLVRASAEAIARGRFFARRIDTLISSFTPAVNAAAPITVTPSQTADRPLDDRAIPA
                     YYTVDMAAARPIHNAWSFARERRAQARFNYSANGPLYEATGAAAAPLKARIDGFSFFR
                     IEGHLGKQVVPAVQQIEARIAQANLPFAVRSVLIGGTRPQIQIKPTIRYTDLHRFHHL
                     IRHDLTSQLDDVMAHNDRFKADVDLAVDGKFLDDRAAGETVSIKGLAANTRQQVQTVA
                     TSAKTKLARPYSAYKAGTWHDDVKETINQSATMRLSLSDVSKTEMTSPLDSLVGNRHV
                     LWAEWLDEIIGQREVQEDEKLLFANFITQHPGADHAAGVPRGGTFVLVYDSAGIVIAD
                     CALSYHWPEASEPEPDQPPLTPPKSRPPIVIDKGWRVLKPVQRQILDSVNPRLDDFQK
                     NYLQTIKDSVQIFTPAKDLVGGIEEPGSILKDPVFKDPGLKVQLGDTIAKQRRVEEIR
                     KLLVPPTLEATKRTQLEQLLKVADSELGQSIKETMRVITTGGIDATTEGAAAAAI"
     gene            complement(2600..5155)
                     /locus_tag="AC16CY_03165"
     CDS             complement(2600..5155)
                     /locus_tag="AC16CY_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3352805.1"
                     /translation="MTASLTLARERPAELGLDQPRLYALGLDRIRTLAGATWTDHNVH
                     DPGITALELLSYTLTDLAYRASFPIEDLLCGSDQFVPISAAKMLPNQPMTILDYRKLM
                     IDVPGIRNAWLEPIELLYFADLTSGEILLQNPNTPGIKDILLRGVYVAKIEFDDPALS
                     NPDKLALQGEVYTRLQAHRNLCEDFAGVSTVSEQRFLLCGEIDLAPDADVAAVHLEIL
                     RQVQDYLAPAVRRYTRDEMLARTKPDGSPYTAADLFDGPLLEHGFIDSAELEAADLRT
                     DVRLSDVISRVMDIAGVRAVRELVIGPEPPPNTEVPIENRWLVPVEPGRRPSLNTAKC
                     RVVYYKGSIPIVPAPVAALPAVAPPPIACEDVPIPGGRRRDLRQYTSVQQHFPAVYGI
                     GANPLPESLPPRRRALARQLKAYLLFFDQVMANFCAQLASMPALFEIATADTPTYFAQ
                     IVSRAPEYLDIYGVEPGDADTLATVLANGVETVAARRARRTRVLDHLIARVAERFQDY
                     AQIMESVFGATAASLLEDRRSFLETCPAIGRDRGLAYDYTQQNALWDTTNVSGLERRL
                     GRLLGIGDVRRRDLTTVAPGADAAITGSAPNVGYTLTDGAAPGAAVLLKEDAALATED
                     DARAQMLRAFELGQWSSTYVRTQRPNGKWFFTIVSKTNEVVGRGEDVDTPEALEEAIV
                     ALRTYLRRHYSREGFYVIENILLRPEQAADASLHFCVDPDCDDCGGDDPYSWRLHVIL
                     PAYAGRFNDMGFRRFVEQTIREEVPAHILPKICWIDEAAMADVQKAYREWLEVRAGLA
                     PGERTARIAALVDALTKVKSVYPVQKLIACGGVSADAKFIIGQTALGTKQSNN"
     gene            complement(5152..8184)
                     /locus_tag="AC16CY_03170"
     CDS             complement(5152..8184)
                     /locus_tag="AC16CY_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR3352806.1"
                     /translation="MADDPTLRNAIARPGQSQPERLPKELGGHFVDVDERTPASLLGY
                     ARSLAGTIPFIGPGNQEQTGGWTAFFDQQAVPEQTKAPMALYDAFLHLYDRGPRAAIN
                     RLTGAHLDFFYQRVLGFRPRPARPDLAHAVITLKKGSPAVRVLPWHLLAAGKDATGAD
                     RFYAPTTAVIVNTSRVASVRSVFVDQASNGQVRFAPVANSSDGLGGPLPEEAPQWRPF
                     GHRELPAADIGFAIASPILRLKEGTRRITVTLTVDNAAALNASALGRAFEAFLTGEKQ
                     WLGPYAVAATLSSSGVLELRIELTGSEPAVADYHVKTHAHAYTTDAPVLQLLLRPGPG
                     LDYGEVSRLRVQKARLSVDVAGVASLTLESDAGTLDPKKAFLPFGPEAAAGSRFLIGC
                     DEALSKSLSRLDLTLQWKGAPVAFATQYAEYSAGFTVNNSAFVASAVFKDRASEHSSV
                     ETLFDPSDATRPFVVQLLSNAAPPSPKATGFYVYALSRARTAWGRLLAQKMVLRHPTS
                     QSFLQQPPEARPGFVSLVLARGFLHQAYRQQSVARIVDRLSNSKLPLLQEPYTPTLQS
                     LALSYTAQTAEVAVSSTSADDFAQFDVQFFHVGPFGQMRDHGHQRAAMAFVTDTQVPL
                     VPVYEQRGELLVGLTELAAGDSVSVLCQVAEGSANPDVPAQPPQWFVLCDNYWKRLGA
                     EGVVRDTTNRLLASGVVTFVIPAEATVENTIMPPGYIWLKAATSQDPESTSKMVDVAA
                     NAVEVQWVMASEAAAAAHLATALPAGSIVKLSTPIPQVVKVTQPFASFGGSPREDENG
                     LRTRAAERLRHRDRAITPWDYERIVLDAFPSVHRVKCIPHARPGAWMTPGHTLIVVVP
                     DLRNRNAPDPLRPKVDADTIVRITDLLRARMPGQARIAVKNPVYQRLRVSCKVRFHTG
                     YEFNFYRGETVRALIRMLSPWAFDASRPLTFGGRVYRSTLLNFIEELPFVDYVTDFTL
                     LTGVDGGTLTAVEYAEPQTPDAILVSDRMHEIGEAVST"
     gene            complement(8185..8628)
                     /locus_tag="AC16CY_03175"
     CDS             complement(8185..8628)
                     /locus_tag="AC16CY_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HIR3352807.1"
                     /translation="MTDDALNAPSFLGRGWSFPPEFVQASGDVVMTEEQADIDASLRV
                     LFGTAAGERFLVPTYGLDLHELQFEPVSTSMRTFLQDRIKTSILVHEPRITLLSLEVR
                     NPTEADGMLLIVVEYAIRATNSRFNLVYPFYWNDATEVAMPTGGR"
     gene            complement(8632..8913)
                     /locus_tag="AC16CY_03180"
     CDS             complement(8632..8913)
                     /locus_tag="AC16CY_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HIR3352808.1"
                     /translation="MPPAARIGDVSNHGGTITGPGVPTVLVGGQPAAGAGDIHVCVLP
                     PNTHQPTVSPFPMGSTTVLVGGKPALRTSDACLCGAMAAVGAPTVLVGG"
     gene            complement(8927..10666)
                     /gene="vgrG"
                     /locus_tag="AC16CY_03185"
     CDS             complement(8927..10666)
                     /gene="vgrG"
                     /locus_tag="AC16CY_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HIR3352809.1"
                     /translation="MATRALPIPATHRGFTVKAAGAEVPRQHQLLAATVVKTVNRVSS
                     ARLVYLDGSASAGKFPLADDALFVPGQEIEVLAGPAGSPVSIFAGVVVRQSVKVRDAS
                     APQLIVECRHKAAKLTVGRKNAYYMDQSDSDIVSALLGDAGLSASVESTPLKHKQQVQ
                     YRSTDWDFLLARARANGKLVLTNDGTVAVKAPTTSTTPSVTLQFGATVLELDAEIDAR
                     VQFAGVKGISWDPAQQSLVESDASAPRVATPGNLTSDDLAAVVGLPSYDLRHAAIVED
                     EAKAWADAEWLHSAMSKVSGRAKCEGVATVNPGDVVSLAGVGERYNGTAFVTGVRHDF
                     DLVSGWKTNVQFGGLSSSPSEDPDRTAPQAGDLLPAVHGMQVGVVTSNEDPDGEHRVR
                     VRLPLVDNDEDGVWARVASLDAGADRGFFFRPEIGDEVVVGFFDDDPRRPVMLGMLHS
                     SANAAPLSGSNDNHEKVYQSRSKLKLSFNDDTKVIEISTPAGNTLTLSEADTAITIAD
                     QNSNTLKMTPDGITVESSKALTLKAGTTLLLESGTSFSMKGGSAVKVEASGSAELSSS
                     GTTTIKGSLVRIN"
     gene            complement(10670..11338)
                     /locus_tag="AC16CY_03190"
     CDS             complement(10670..11338)
                     /locus_tag="AC16CY_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIS tube protein"
                     /protein_id="HIR3352810.1"
                     /translation="MADRGTLERLVIKAYEAPDYSGSPVATFEAYVNPNEITLAYEIE
                     YDAAQGAGTTGSRMNFKNMKPGDLSLTFFIDGTGANGRPIDVQQRVESFQTATGYNGK
                     IHRPNYLKVVWGTLQVKRCVLKSASIAYKMFKADGVPLRAVITATFTDNSDDQTRVAL
                     AQDESPDLTHVRLVAAGDTLPLLCSRIYGDPLRYLQVARANGLDDFRRLEPGTRLVFP
                     PLER"
     gene            complement(11341..11508)
                     /locus_tag="AC16CY_03195"
     CDS             complement(11341..11508)
                     /locus_tag="AC16CY_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HIR3352811.1"
                     /translation="MPIIVDEIVISVEVSNQAAGGAGPASAPTPTRDRQNTVEECVER
                     VLQILREREER"
     gene            complement(11512..11955)
                     /locus_tag="AC16CY_03200"
     CDS             complement(11512..11955)
                     /locus_tag="AC16CY_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3352812.1"
                     /translation="MPNYYPPVSFHFRVDVLGLSKGNDVRFSEVSGLSLEVLTEEVAE
                     GGENRFVQKYPGRVKYPELLLKRGYLVKSDVFGWIRDCVERDQIEPRNVDVMLLNEAH
                     EPLVTWHVVNAFPTKWATSDLNASSNTVVIETLQLYYQHYSVDRS"
     gene            complement(11984..12415)
                     /locus_tag="AC16CY_03205"
     CDS             complement(11984..12415)
                     /locus_tag="AC16CY_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3352813.1"
                     /translation="MPQYPIPVFHFTVEWGGTRVGFSEVTGLTQENQAIEYRDGSFPE
                     YSSIKMPGIRKFSNVTLKRGIVKGDNQFFKWLSTVKLNTVERRNLVISLLNEEHQPVM
                     VWKVMNAFPVKVEGPQLKAAGNEVAIESIEIAHEGLELQNE"
     gene            complement(12439..13878)
                     /locus_tag="AC16CY_03210"
     CDS             complement(12439..13878)
                     /locus_tag="AC16CY_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HIR3352814.1"
                     /translation="MPTYKTPDVYIEEIPVFPPSVAEVGTAIPAFVGYTEKATKKGND
                     LTLKPTKLYSLAEYEELFGGPHPEQINVVLATDANAPNGLKVTYSVGTGATLPAYLLY
                     WAVKMYYDNGGAQCYVVSVGDYGDAIAKGDDDTGLQGGLVQVGLQDEPTLLVVPEATR
                     LAFADYQSVAQAMLAQCGKLKDRFAILDVWGGETKLGDDVDIGNNTDKEVVLANRDAW
                     ATNLKYGAAYYPFLKTAINHHVALDANGVVTNFKVTLPGAQAAVDLSSIEATNTKEFN
                     VAKAAVNDHFLTLPPSGAVAGVYAAVDGARGVWKAPANVALASVLEPVVRIDNLLQEG
                     LNVDPTTGKSINAIRAFSGRGTVVWGARTLAGNDNEWRYVSVRRFFNMVEESVKKSTY
                     FAVFEPNDATLWVKVRGMIENYLIDKWREGALAGALPKDAFYVKCGLGTTMSAQDILE
                     GRLHVEIGMAVVRPAEFIVLKFFHKLQTS"
     gene            complement(13891..14907)
                     /locus_tag="AC16CY_03215"
     CDS             complement(13891..14907)
                     /locus_tag="AC16CY_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352815.1"
                     /translation="MITRFIVLCTLDVTHVYYGGSCRDFTFVIPPDVGALVRRGRLLP
                     RTLDGKLYVLFEADENDAPRTPLPGAVMRIGLCLNNTSFSNFTALAFSPGSAVAIYGN
                     GAGNAQLGAPTTAVLSGHLLTVPLAQGDRPVTVTASPTGGAAPPPIEVTTAADRDSVS
                     FDLTGFAPGLYTVVEAYPGNVEHSRAFYVDPELAREPLFGVVEITIAAGFYAAAPAFS
                     VAFDARAETLKYYVVARGYTDAEFNQIDVSQLSANGPPEITFTKKLPAAFTPSDLPAS
                     LVAPGGAKLALFQSTAPVKRKAGGYARVQLRRQNDVIISNLPQVTPDRPNADLIVHLS
                     KPKP"
     gene            complement(14904..15473)
                     /locus_tag="AC16CY_03220"
     CDS             complement(14904..15473)
                     /locus_tag="AC16CY_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pvc16 family protein"
                     /protein_id="HIR3352816.1"
                     /translation="MIDVALKFLAGAVNAYLFARTGSPQGSLAFGRLVDDTGKCTIAD
                     QSLGAALVNIDEERVLKSHQPDTVLINGRQVVLQPELKLNLTILIAANFTTYEIALRQ
                     LSWVLTFFQSRPGFTRDRYPALDPRIERFTVELQSLTFEQLNQLWAAVGGKQLPSVVY
                     RLRLVAVQDVEPSSVQPPVSEIAAAIHGL"
     gene            complement(15470..15718)
                     /locus_tag="AC16CY_03225"
     CDS             complement(15470..15718)
                     /locus_tag="AC16CY_03225"
                     /inference="COORDINATES: protein motif:HMM:NF023376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR3352817.1"
                     /translation="MTEERRSPAHRRYANWLDVSFSAEEFILDFGQRFGDADSVLHSS
                     IVATPRSVDAFVDVLARSLEEYRRRYGAPGSREGAIRP"
     gene            complement(15812..17128)
                     /locus_tag="AC16CY_03230"
     CDS             complement(15812..17128)
                     /locus_tag="AC16CY_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3352818.1"
                     /translation="MSINWSATDRVAAPRVFVTGRDAPERERIARMLVELGADLCADR
                     ADLVIVLESNGHPLVGLREICQRFRASPVIFVAYSGSEHLAVEAFRSGANDYLRIPIT
                     PTGLAESLRRLHIEPRDTDVPGLIGESPHTQNLRLVIKRIAVTPCSVLISGETGTGKD
                     LIARLIHESSPRAQGPFVTINCSALPETLFESELFGYERGAFTGAQATSIGRLQAASG
                     GTVLLDEVGELSLTTQPKLLRAIDQREVQRLGSRRHETIDVRWVAATNRNLQELVRSG
                     LFRADLFYRLNVYSLVVPPLRERPDDVPVLARAFLRDCAPAYGRLGAAFTDEAVARLA
                     AYEWPGNVRELRNVVESSVLTTESDAIGIEALPPAVRGADFSTPLPVETERVRVVAAL
                     RASHGNKSEAAQRLNWSRMTLYRKLARHRIDVTSECDSVTAKLPRE"
     gene            complement(17279..17740)
                     /locus_tag="AC16CY_03235"
     CDS             complement(17279..17740)
                     /locus_tag="AC16CY_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3352819.1"
                     /translation="MPVVHALIQTREDLDRIASDVPPEHVWLRPGGAASIGFHVRHTG
                     GALDRLFTYARGEALSDAQRAALREEGAAGDPPAPLASVVAGVGAIIDRALDQLRATP
                     RESLFDERKLGRAGLPTNVIGLLFHAAEHCTRHVGQAITTAKILKAQTPSR"
     gene            complement(18155..>18598)
                     /locus_tag="AC16CY_03240"
     CDS             complement(18155..>18598)
                     /locus_tag="AC16CY_03240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3352820.1"
                     /translation="CRLFADMNRLPLADATADVVFAASCVHHSWDLGRTFREIARVLK
                     PGGAAYLCGEPLPSLVRFVVGGGFGRRERELGINETWIRRGSWLRRCRQSGLEPRIVY
                     PGLNGGQLRERLRRKGLPGFLAGLIRPVLPILQVSVHLRADKPRT"
BASE COUNT         2933 a         6184 c         6375 g         3106 t
ORIGIN      
        1 gatcgcggcc gcggccgcgc cctctgtcgt ggcatcgatg ccgccggtcg tgatgacgcg
       61 catggtctcc ttgatcgact ggccaagctc ggaatcggcc accttcagca gctgctccag
      121 ctgcgtacgc ttcgtcgctt cgagcgtcgg gggcaccagc agcttgcgga tctcctcgac
      181 gcgccgctgc ttggcaatcg tgtctcccag ctgcactttc aacccgggat ccttgaacac
      241 gggatccttg aggatcgacc ccggctcctc gatcccgcca accagatcct tggccggggt
      301 gaagatctgg accgagtcct tgatcgtctg caggtagttc ttctggaagt cgtccagccg
      361 cgggttgacg ctgtcgagga tctgccgctg caccggcttc agcacgcgcc agcccttgtc
      421 gatcacgatg ggcggccggc tcttcggcgg cgtgagcggc ggctgatccg gttcgggctc
      481 cgacgcttcc ggccagtggt acgacagcgc gcagtcggcg atgacgatgc cggcgctgtc
      541 gtagacgagc acgaaggtgc cgccgcgcgg tacgccggcg gcgtggtccg cgcccgggtg
      601 ctgcgtgatg aagttggcga acagcagctt ctcgtcctcc tggacctcgc gctggccgat
      661 gatctcgtcc agccattcgg cccacaggac gtgccggttg ccgaccagcg agtccagcgg
      721 cgacgtcatc tcggttttcg acacatcact caggctcagg cgcatcgtgg ccgactggtt
      781 gatcgtctcc ttgacgtcgt cgtgccaggt gccggccttg tatgcgctgt agggacgcgc
      841 cagcttggtc ttcgcgctcg ttgccaccgt ctggacctgc tgacgcgtgt tcgcggcgag
      901 gcccttgatc gacacggtct cgccggcggc gcgatcgtcg aggaacttgc cgtccacggc
      961 caggtccaca tccgccttga accggtcgtt gtgcgccatc acgtcgtcga gctgcgacgt
     1021 gaggtcgtgg cgaatgaggt gatgaaaccg gtgcagatcg gtgtagcgga tggtgggctt
     1081 gatctggatc tgcgggcgtg tgccgccgat cagcaccgag cgcaccgcga acggcaggtt
     1141 ggcctgcgcg atcctggcct cgatctgctg gacggccggt acgacctgct tgccgaggtg
     1201 cccttcgatc ctgaagaacg agaacccgtc gatccgcgcc ttcaacggcg cggccgccgc
     1261 tccggtggct tcatagagcg gcccgtttgc cgaatagttg aaccgcgcct gcgcgcgacg
     1321 ttcgcgagca aagctccagg cgttgtgaat cggccgcgcg gcagccatgt ccaccgtgta
     1381 gtaggcgggg atcgcgcggt cgtccagtgg gcgatcggcc gtctgactgg gcgtgacggt
     1441 gatcggcgcc gcggcgttca cggccggggt aaagctggag atcagggtgt cgatccgcct
     1501 ggcgaagaag cgcccgcgcg cgatcgcctc ggcggaagcg cgcacgagcg gcgacggata
     1561 gaagccggtt ctgagcaacg gcggcgggct gggcacgccc acgacgccga gcaggaggtg
     1621 cttcgggaag agatcgagcg ccggacacag gagtgaggtc tcgccggcga gcgactcgcc
     1681 gagcgcgttg tacgcgtcca cgagatcgct gagaaagtcg tagtaggcct ggatgcccgt
     1741 gtcgggaacg gcggtgtcca gcttggcaat ccagccgggc gccgggttgc cggtgaacac
     1801 ctcgccgagc atgccggcgg cgctgacgac ctggccctgc agcgcctgct tcagtcgatc
     1861 gcgggtggcg ttggctgccg tccggtacgc ttgattgagc gccgccgccg agccggtctg
     1921 ggcgctgagc gcgacgcgat ccgcaaagag cggcgagaac gcctgcgaca acgtgtcgag
     1981 cgtcgcgatc ttcgagatca acggtccgat gtcgttctga tcgagcacca gcagcctgac
     2041 gttgccgagg gcgtccttgc ccttgttgtc gcagtcgccg cccgtgcaga ggtcctgatc
     2101 cttgaggaag ctctccatca ggagaagggc gaccatctcg tcgagcttct tgcccgttgc
     2161 cgtgctgaag gtgtcgagtg tcgccgcggt gtccacttcc tccggcacca gctcgaatac
     2221 cttcaccacc atgctctggc cgtcgtagaa cgggtcgtac ctgggggcag tctcgtcgta
     2281 cgtgcgaaac ctcgtgtact cgaggtcccg gggcacgcgg atcagatcgc cgtcggtcgt
     2341 gacgccgata cctttcgtca gcacgaccac attgttgctg accgacaggc gcagcccggc
     2401 gacgatgccg acgcccatca gggccacacg cgtgagccgc tgatgatcgt cgaggtagtc
     2461 cacaagcccg ttcagcggct ccttggtcag gacctggttg tcctcgaaca ccttgtagcc
     2521 ggtcgacacg tcggtgagat gctgaatgac gctggccatg attgctcccg gtccggctga
     2581 agccggacac tacgtacgtc tagttgttgc tctgcttcgt tccgagtgct gtctgcccga
     2641 tgatgaactt ggcgtcggcg ctgacgccgc cgcaggcgat cagcttctga accgggtaca
     2701 cgctcttcac cttcgtgagc gcgtcaacga gcgccgcgat gcgcgccgtc cgctcgccag
     2761 gcgcgaggcc ggcgcggacc tcgagccact cgcgatacgc cttctgcacg tcggccatgg
     2821 ccgcctcgtc gatccagcag atcttcggca ggatgtgggc cggcacttcc tcgcggatcg
     2881 tctgctcgac gaagcggcgg aatcccatgt cgttgaagcg gccggcgtac gccggcagga
     2941 tcacgtgaag gcgccaggag tacgggtcgt cgcccccgca gtcgtcacag tcgggatcga
     3001 cgcagaagtg cagcgacgcg tcggcggcct gctcgggccg cagcaggatg ttctcgatga
     3061 cgtagaaccc ctcgcggctg tagtggcggc gaagataggt gcgcagcgcg acgatggcct
     3121 cctcgagcgc ctccggggta tcgacgtcct cgcctcggcc cacgacctcg ttggtcttcg
     3181 acacgatcgt gaagaaccac ttcccgttcg gtctctgcgt ccgcacgtag gtcgagctcc
     3241 actgccccag ctcgaacgca cgcagcatct gcgcgcgcgc atcgtcctcg gtggccaaag
     3301 cggcgtcttc cttgagcagg actgcggcgc cgggagcggc gccgtcggtc agcgtgtagc
     3361 cgacattcgg cgccgatccg gtgatcgccg cgtcggcgcc cggcgccacg gtcgtcagat
     3421 cgcgccgccg cacgtcgcct atgccgagca ggcgtccaag gcgcctctcg aggccggaga
     3481 cgttggtcgt atcccagagc gcgttctgtt gggtgtagtc gtaggcgagg ccgcgatccc
     3541 gcccgatcgc ggggcacgtt tcgaggaagc tacgccggtc ctccagcagg ctggcggcgg
     3601 tggcgccgaa caccgactcc atgatctgcg cgtagtcctg gaaccgctcg gcgacgcgcg
     3661 cgatcagatg gtccagaacg cgcgtgcgcc gcgcgcggcg cgccgcgacg gtttccacgc
     3721 cgttggccag cactgtcgcc agcgtgtcgg cgtcgcccgg ctcgacaccg tagatgtcca
     3781 ggtactccgg cgcgcggctg acgatctgcg cgaagtaggt cggcgtgtcg gcggtggcga
     3841 tctcgaacag cgccggcatg ctggcgagct gggcgcagaa gttcgccatc acctgatcga
     3901 agaacagcag atacgccttg agctgccgcg cgagcgcgcg ccgccggggg ggaaggcttt
     3961 cgggcagcgg gttcgcgccg atgccgtaca cggccggaaa gtgctgctgg accgaggtgt
     4021 actgccgcag gtctctccgg cggccgccgg gaatgggcac gtcctcgcag gcgatcggcg
     4081 gcggcgcgac ggctggaagc gccgcgacgg gagccggcac gatcgggatg ctgcccttgt
     4141 agtagacgac gcggcacttt gccgtgttga gcgagggccg cctgcccggt tcgaccggca
     4201 ccagccatcg gttctcgatc gggacctccg tgttcggagg cggctccggg ccgatcacca
     4261 gctcgcggac cgcgcggacg cccgcgatgt ccatcacgcg gctgatgacg tccgacaggc
     4321 gcacgtccgt ccgcagatcg gcggcctcga gctcggcgct gtcgatgaag ccgtgctcga
     4381 gcagcggacc gtcgaacaga tcggccgcgg tgtagggcga gccgtccggc ttcgtcctgg
     4441 cgagcatctc gtcgcgggtg tagcggcgga ctgccggcgc gagatagtcc tgcacctggc
     4501 gcaggatctc caggtggacg gcggcgacgt cggcgtccgg cgcaaggtcg atctcgccgc
     4561 acagcaggaa gcgctgctcg ctgacggtgc tgacgccggc gaagtcttcg cacaggttgc
     4621 ggtgcgcctg gaggcgcgtg tacacctcgc cctggagcgc gagcttgtcg ggattcgaga
     4681 gcgccggatc gtcgaactcg atcttcgcga cgtagacgcc ccgaaggagg atgtccttga
     4741 tcccgggcgt gttcgggttc tggagcagga tctcgccgct cgtgaggtcg gcgaagtaga
     4801 gaagctcgat cggctccagc caggcgttgc ggatgccggg cacgtcaatc atcagcttcc
     4861 ggtagtcgag gatcgtcatc ggctggttcg gcagcatctt cgccgccgag atcggcacga
     4921 actgatcgct gccgcagaga aggtcctcga tcgggaagga cgcgcggtac gccagatcgg
     4981 tcagcgtata agacagcagc tcgagcgccg tgatgcctgg atcgtggacg ttgtgatcgg
     5041 tccacgtcgc gccggcaagc gtccggatac gatcgagccc gagcgcgtag agccggggct
     5101 ggtcgagacc gagctcggcc ggccgctccc tggcgagcgt gagcgaggcc gtcatgtgct
     5161 caccgcctcc ccgatctcgt gcatgcggtc cgacacgaga atcgcatccg gcgtctgcgg
     5221 ctcggcgtac tcgacggccg tcagcgtccc gccgtcaacg ccggtgagca gcgtgaagtc
     5281 ggtcacgtaa tcgacaaacg gcagctcctc gatgaagttc agcagcgtgg atcgatagac
     5341 acggccgccg aaggtcaggg gccggctggc gtcgaacgcc cacggggaca gcatccggat
     5401 gagggcgcgc accgtctcgc cccggtagaa gttgaactcg taccccgtgt ggaacctgac
     5461 cttgcacgac acgcgaaggc gctggtagac cggattcttc acggcgatcc gtgcctgccc
     5521 gggcatgcgc gcgcggagca ggtccgtgat ccggacgatg gtgtcggcat ccaccttcgg
     5581 ccggagcgga tcgggcgcgt tccggttgcg caggtcggga acgaccacga tcagcgtgtg
     5641 accgggcgtc atccaggctc ccggccgcgc gtgcggaatg cacttcacgc gatgcaccga
     5701 cggaaacgcg tcgagcacga tgcgctcgta gtcccagggc gtgatcgccc ggtctctgtg
     5761 ccgcaggcgc tcggccgccc gcgtgcgcag cccgttttcg tcctcccggg ggctgccgcc
     5821 gaacgaggca aacggctgcg tgaccttgac gacctgcggg atcggcgtgg acagcttgac
     5881 gatcgagcca gccggcagcg ccgtcgccag gtgggccgcg gccgcggcct cggaggccat
     5941 cacccactgc acctccacgg cgttggcggc gacgtcgacc atcttcgagg tggactcggg
     6001 gtcctgggac gtggcggctt tcagccagat gtagcccggc ggcatgatcg tgttctccac
     6061 ggtcgcttcc gccggaatca cgaaggtcac caccccgctg gcgagcagcc gattggtggt
     6121 gtctcgaacc acgccctccg cgccgagccg cttccagtag ttgtcgcaca gcacgaacca
     6181 ttgcggaggc tgagccggca catcggggtt cgcgctgccc tcggcgacct gacacagcac
     6241 gctcacgctg tctcccgccg ccagctccgt gagcccgacg agcagctcgc ctcgctgctc
     6301 gtagaccggc accagcggca cctgggtgtc ggtgacaaac gccatcgccg cccgctgatg
     6361 cccgtggtcg cgcatctgcc cgaagggacc cacgtgaaag aactggacgt cgaactgcgc
     6421 gaagtcgtcc gcgctcgtcg aggacacggc gacctcggcc gtctgcgcgg tatacgagag
     6481 cgccaggctc tggagcgtcg gcgtgtacgg ctcctgcaga agcggcagct tcgagttcga
     6541 caggcgatcg acgattcggg ccaccgactg ctgccggtac gcctgatgaa ggaagccgcg
     6601 cgccagcacc aggctgacaa aacccggacg cgcctcgggc ggctgctgga gaaacgattg
     6661 cgacgtcgga tgcctgagca ccatcttctg cgcgagcagc cggccccacg cggtgcgcgc
     6721 ccggctgagc gcgtacacgt agaacccggt cgccttcggg ctgggaggcg cggcgttcga
     6781 gagcagctgc acgacgaacg gacgcgtggc atccgatgga tcgaacagcg tctcgacgga
     6841 ggaatgctcg gacgcgcggt ccttgaacac cgccgaggcg acgaacgcgc tgttgttgac
     6901 ggtgaatccc gcgctgtact cggcgtactg cgtcgcaaac gccaccgggg cgcccttcca
     6961 ctgcagcgtc aggtccagcc gcgagaggct cttcgacaac gcctcgtcgc agccgatcag
     7021 aaagcgcgag ccggcggcgg cctcggggcc gaacggcagg aacgccttct tcggatcgag
     7081 cgtcccggcg tcgctctcga gggtgaggct cgcgacgccg gcgacatcca ccgagagccg
     7141 ggctttctgc acgcgaagac ggctcacctc gccgtagtcg agcccgggcc ccggcctgag
     7201 cagcagctgc aggaccgggg catccgtcgt gtaggcgtgc gcgtgtgtct tgacgtggta
     7261 atcggcgacg gcgggctcgc tgcccgtcag ctcgatgcgg agctcgagaa cgccggagga
     7321 ggacagcgtc gccgcgacgg cgtagggacc gagccactgc ttctcgcccg tcaggaacgc
     7381 ctcgaatgca cggccgagcg ccgacgcgtt gagcgctgcg gcgttgtcca ccgtgagcgt
     7441 cacggtaatc ctgcgggtgc cttctttcag gcgcaggatg ggcgaggcga tggcaaagcc
     7501 gatgtcggct gccgggagct cgcgatgacc gaagggccgc cattgggggg cttcctcggg
     7561 cagcggcccg ccaaggccgt ccgaagagtt cgccaccggc gcgaagcgca cctgcccgtt
     7621 cgaggcctgg tccacgaaga ccgatcgcac cgacgcgacc cgtgacgtgt tgacgatcac
     7681 cgcggtggtc ggcgcgtaga aacgatcggc cccggtcgcg tccttgccgg cggcgagcag
     7741 gtgccacggc agcacgcgaa cggccggcga acccttcttc aaggtgatca cggcgtgcgc
     7801 gagatcgggg cgcgccggcc gcggccggaa gccgagcacg cgctggtaga agaaatcgag
     7861 atgcgcgccg gtcaggcggt tgattgccgc gcgagggccc cggtcgtaca ggtggagaaa
     7921 ggcgtcgtac aacgccatcg gagccttcgt ctgctccgga accgcctgct gatcgaagaa
     7981 ggcggtccac cctcccgtct gctcctggtt gcccggtccg ataaacggaa tcgttcccgc
     8041 cagcgagcgc gcataaccca acagagacgc cggcgttcgc tcgtccacgt ccacgaagtg
     8101 gccgccgagc tctttcggca ggcgctcggg ctggctctgg ccaggccgcg cgatcgcgtt
     8161 ccgcagggtc gggtcgtcag ccactcagcg ccccccggtc ggcatcgcaa cctccgtcgc
     8221 gtcgttccag tagaacgggt agacgaggtt gaacctggag ttggtcgcgc ggatcgcgta
     8281 ctccacgacg atgagcagca tgccgtcggc ttcggtcggg ttgcgcacct cgagcgacag
     8341 cagcgtgatg cgcggctcgt gcacgaggat ggacgtcttg atgcggtcct gcaggaacgt
     8401 ccgcatcgac gtgctcaccg gctcgaactg cagctcgtgc agatccagcc cgtaggtcgg
     8461 caccagaaag cgttcgcccg ccgcggtgcc gaagaggacg cgcaggctgg cgtcgatgtc
     8521 ggcctgctct tcggtcatca cgacgtcgcc ggacgcctgg acgaactccg gcgggaagct
     8581 ccatccgcgg cccagaaacg atggcgcgtt cagcgcgtcg tcagtcatcg cctaccctcc
     8641 caccagcacc gtgggcgcgc ccacggccgc catcgctccg cacaggcacg cgtccgacgt
     8701 gcgcagcgcc ggcttgccgc cgacgagcac cgtcgtgctc cccatcggaa acggactgac
     8761 ggtcggctgg tgcgtgttgg gcggcagcac gcagacgtgg atgtcgcccg cgcccgcggc
     8821 cggctgcccg ccgacgagca ccgtcggcac gcccgggccg gtgatcgtgc cgccgtgatt
     8881 gctgacgtct ccgatgcgcg cggctggtgg catccggcat ctccgctcaa ttgatccgca
     8941 ccagcgagcc cttgatcgtc gtcgtccccg agctcgagag ctcggccgac cccgacgctt
     9001 cgaccttcac ggcgctgccc cccttcatgc tgaacgaggt ccccgactcg agcaggagcg
     9061 tggtgcccgc cttcagcgtg agcgccttgc tgctttcgac cgtgatgccg tcgggcgtca
     9121 tcttcagcgt gttgctgttc tggtcggcga tcgtgatcgc cgtgtcggcc tcgctgagcg
     9181 tgagggtgtt gcccgccggc gtcgagatct cgatgacttt cgtgtcgtcg ttgaacgaca
     9241 gcttcaactt cgagcggctc tggtacacct tctcgtggtt gtcgttggag ccgctgagcg
     9301 gcgcggcatt ggcgctgctg tgcagcatcc ccagcatcac cgggcgccgg gggtcgtcgt
     9361 cgaagaagcc gacgacgact tcgtcgccga tctcgggccg gaagaagaac ccgcgatcgg
     9421 ccccggcgtc gagactggcc acgcgcgccc agacgccgtc ttcgtcgttg tccacgagcg
     9481 gcagcctgac gcgaacgcgg tgctcgccgt cgggatcctc gttgctggtc acgacgccaa
     9541 cctgcatgcc gtgcaccgcg ggcagcagat cgccggcctg cggcgcggtc cggtcgggat
     9601 cctccgacgg cgaggacgac aggcctccaa actgcacgtt ggtcttccag cccgagacga
     9661 ggtcgaagtc gtggcgcacg cccgtcacga acgccgtgcc gttgtaccgc tcgccgacgc
     9721 cggcgagcga cacgacgtcg cccgggttca ccgtcgcgac gccctcgcac ttggcgcggc
     9781 cgctcacctt gctcatcgcg gagtgcagcc actcggcgtc ggcccacgcc ttggcttcgt
     9841 cttcgacgat cgccgcgtgc cggaggtcgt agctcgggag gccgacgacc gccgcgaggt
     9901 cgtcgctcgt caggttgccc ggcgtggcga cgcgcggggc ggaggcgtcg ctctcgacga
     9961 ggctctgctg ggcagggtcc caggagatgc ccttgacgcc ggcgaactgg acgcgcgcgt
    10021 cgatctccgc gtcgagctcg aggaccgtcg cgccgaactg cagcgtgacg ctcggcgtcg
    10081 tgctggtcgt cggcgccttg accgcgaccg tcccgtcgtt ggtcagcacg agcttcccgt
    10141 tggcgcgcgc cctggccagc aggaagtccc agtcggtcga tcggtactgg acctgctgct
    10201 tgtgcttcag cggcgtgctc tcgacgcttg ccgagaggcc cgcgtcgccg agaagggccg
    10261 agacgatgtc gctgtcgctc tggtccatgt agtacgcgtt cttgcggcca acggtcagtt
    10321 tggctgcctt gtgccggcac tcgacgatga gttggggcgc gctggcgtcg cgcaccttca
    10381 ccgattgacg gacgacgacg cccgcgaaga tcgagaccgg gctgccggcc ggtcccgcca
    10441 gcacctcgat ctcctggccc ggcacgaaca gggcgtcgtc ggcgagcggg aactttccgg
    10501 ccgacgcgga cccgtcgaga tacacgagcc gcgcggacga gacgcggttg accgtcttga
    10561 cgaccgtcgc cgcgagcagc tggtgctggc gcggcacctc cgcgccggcg gccttgacgg
    10621 tgaatccgcg gtgcgtggct gggatcggca gcgcgcgggt ggccacgggt tatcgctcca
    10681 gcggcgggaa cacgagacgc gtgcccggct cgagacggcg gaagtcgtcc aggccgttcg
    10741 cgcgcgcgac ctgcagatac cgcagcgggt cgccgtagat gcgcgagcag agcagcggca
    10801 gcgtgtcccc ggccgcgacg agccgtacgt gcgtcaggtc cggcgactcg tcctgcgcga
    10861 gcgccacgcg ggtctggtcg tcggagttgt cggtgaacgt ggccgtgatc acggcgcgca
    10921 gcggcacgcc gtcggccttg aacatcttgt aggcgatcga cgccgacttg agcacgcagc
    10981 gcttgacctg cagcgtgccc cagacgacct tcaggtagtt gggccgatgg atcttcccgt
    11041 tgtagccggt ggccgtctgg aaggactcga cgcgctgctg cacgtcgatc gggcggccgt
    11101 tggcgcccgt cccgtcgatg aagaaggtga gcgagaggtc gcccggcttc atgttcttga
    11161 aattcatccg gctgccggtc gtgcccgcgc cctgcgccgc gtcgtactca atctcgtacg
    11221 ccagcgtgat ctcgttcggg ttgacgtagg cctcgaacgt ggcgacgggc gacccggagt
    11281 agtcgggcgc ttcgtaggcc ttgatcacca ggcgctcgag cgtgccccga tcggccacgg
    11341 ctaccgctcc tcccgctcgc ggaggatctg caggacgcgc tcgacgcatt cctcgacggt
    11401 gttctgccgg tcccgggtcg gcgtcggcgc cgatgcgggc ccggcgcccc cggcagcctg
    11461 gttggatacc tcaacggaaa tcacgatctc gtccacgatg atgggcatgg ctcacgaccg
    11521 gtcgacgctg tagtgctggt aatagagctg cagcgtctcg atgacgacgg tgttggacga
    11581 ggcgttcagg tcgctcgtgg cccacttggt cgggaacgcg ttcacgacgt gccaggtcac
    11641 cagcggctca tgggcttcgt tgagcagcat cacgtccacg tttctcggct cgatctggtc
    11701 gcgctcgacg cagtcgcgaa tccacccgaa aacatccgac ttcaccaggt acccgcgctt
    11761 cagcagcagc tcgggatact tcacgcggcc agggtacttc tggacgaacc ggttctcgcc
    11821 gccctcggcc acctcctccg tgagcacctc gagcgacagg ccgctcacct cgctgaagcg
    11881 gacgtcgttg cccttcgaca ggccgagcac gtccacgcgg aagtggaacg agacgggcgg
    11941 gtagtagttc ggcattcagc cccccagggt cgatcggccc gcgctactcg ttctgcagct
    12001 cgaggccctc gtgcgcgatc tcgatcgact cgatcgcgac ctcgttgcca gccgccttca
    12061 gctgcggccc ctcgaccttg acgggaaagg cgttcatcac cttccacacc atcaccggct
    12121 ggtgctcctc gttgagcagg ctgatcacga ggttccgccg ctcgaccgtg ttgagcttca
    12181 ccgtgctcag ccacttgaag aactggttgt cgcccttgac gatgccgcgc ttcagcgtca
    12241 cgttgctgaa cttgcggatg cccggcatct tgatcgacga gtactcgggg aacgagccgt
    12301 cgcgatactc gatcgcctgg ttttcctgcg tgaggccggt gacctcggag aaccccactc
    12361 gcgtgccgcc ccactcgacg gtgaagtgaa agactggtat cggatactgc ggcatgatgc
    12421 ctactccttc tcacgacctc aggaggtctg cagcttgtgg aagaacttga ggacgatgaa
    12481 ctcggccggc cgcacgacgg ccatgccgat ctcgacgtgc aggcgtccct cgaggatgtc
    12541 ctgcgccgac atcgtcgtgc cgaggccgca cttgacgtag aaggcgtcct tcggcagcgc
    12601 gccggcgagc gcgccctcgc gccacttgtc gatcaggtag ttctcgatca tgccgcgcac
    12661 cttgacccac agcgtggcgt cgttgggctc gaacacggcg aagtaggtcg acttcttcac
    12721 cgactcctcc accatgttga agaagcggcg caccgacacg taacgccact cgttgtcgtt
    12781 gccggccagc gtgcgggcgc cccacaccac cgtgccgcgt ccgctgaagg cgcggatggc
    12841 gttgatcgac ttgcccgtcg tgggatcgac gttcaacccc tcctgcagca ggttgtcgat
    12901 cctgaccacc ggctcgagga cgctcgccag cgccacgttc gccggcgctt tccagacgcc
    12961 gcgcgcgccg tcgacggcgg cgtagacgcc cgccacggcg ccgctcggcg gcaaggtgag
    13021 gaagtgatcg ttgaccgcgg ccttcgccac gttgaactct ttggtgttgg tcgcttcgat
    13081 cgagctgaga tcgacggcgg cttgcgctcc agggagcgtg accttgaaat tcgtgacgac
    13141 gccgttcgcg tcgagggcga cgtggtggtt gatcgcggtc ttgaggaacg gatagtacgc
    13201 cgcgccgtac ttcaggttcg tggcccaggc gtcgcggttg gcgagcacga cctccttgtc
    13261 cgtgttgttg ccgatgtcga cgtcgtcgcc gagcttcgtc tcgcctcccc agacatcgag
    13321 gatcgcaaag cggtccttga gcttcccgca ctgcgcgagc atcgcctgcg ccaccgactg
    13381 gtagtcggca aacgccagcc gcgtcgcttc ggggacgacg agcagcgtcg gctcatcctg
    13441 caggcccact tgaaccaggc cgccctgcag gcccgtgtcg tcgtcccctt tcgcaatcgc
    13501 gtccccgtag tcgccgaccg agacgacgta gcactgggcc ccgccgttgt cgtagtacat
    13561 cttgacggcc caatagagga ggtaggcggg cagcgtggcc cccgtaccca cgctgtaggt
    13621 caccttcagg ccgttgggcg cgttcgcatc ggtcgccagg acgacgttga tctgttccgg
    13681 gtgaggtcct ccgaagagct cctcgtactc cgccagtgag tacagcttcg tcggcttcaa
    13741 cgtgagatcg ttgcctttct tcgtcgcctt ctcggtgtat ccgacaaacg cggggatggc
    13801 agtgcccacc tcggcaaccg acggtggaaa gacaggtatc tcttcgatgt acacatcggg
    13861 cgtcttgtag gtaggcatcg tcccgacctc ctaaggcttg ggtttcgaca ggtgcacgat
    13921 cagatcggcg ttgggccgat cgggcgtcac ctgcggcaga ttgctgatga tgacgtcgtt
    13981 ctgccgacgg agctggaccc gggcgtagcc gcccgccttc cgcttcacgg gcgcggtcga
    14041 ctgaaacagc gccagcttgg cgccgccggg cgcgaccagg gatgccggca ggtccgacgg
    14101 cgtgaaggcc gccggcagct tcttcgtgaa ggtgatctcc gggggtccgt tggcggagag
    14161 ctgcgacacg tcgatctgat tgaactccgc gtcggtgtag ccgcgcgcga cgacgtagta
    14221 cttcagggtc tccgcgcgcg cgtcgaaggc cacgctgaag gccggcgcag cggcgtagaa
    14281 tccggccgcg atcgtgatct cgacgacgcc gaacagcggc tcccgcgcga gctcgggatc
    14341 gacatagaac gcgcggctgt gctcgacgtt gcccggatac gcctccacaa ccgtgtacag
    14401 ccccggggcg aagccagtga ggtcgaacga caccgaatcc cggtctgccg cggtcgtgac
    14461 ctcgattggc ggcggcgcgg cgccgccggt gggcgaggcg gtcaccgtca ccgggcggtc
    14521 gccctgagcg agcgggaccg taaggaggtg tccggagagc accgcggtcg tcggggcgcc
    14581 gagctgcgcg ttgccggcgc cattgccgta gatcgcaacg gcggatcccg gcgaaaacgc
    14641 gagcgcggtg aagttgctga aggacgtgtt gttcaggcac agcccgatcc gcatgacggc
    14701 gcccggcagc ggggtccgcg gcgcatcgtt ttcatcggct tcgaagagca cgtacagctt
    14761 tccgtccagc gtgcgcggca gcagccggcc tcggcgcacg agggctccga cgtcgggagg
    14821 gatcacgaac gtgaagtcgc ggcacgagcc gccgtagtag acgtgcgtga cgtcgagcgt
    14881 gcagaggacg atgaagcgcg tgatcatagg ccgtgaatcg cagccgcgat ctcggacacg
    14941 ggcggctgaa cgctgctcgg ctcgacgtcc tggacggcaa cgagccgcag ccggtacacg
    15001 accgacggca attgcttgcc gccgacggcg gcccacaact ggttcaactg ttcgaaggtg
    15061 agcgactgca gctcgaccgt gaagcgttcg atccgcggat cgagcgccgg gtaccggtcc
    15121 cgggtgaagc ccggacggga ctgaaagaag gtcagcaccc aggacagctg gcgcagcgcg
    15181 atctcatacg tcgtgaaatt ggccgcgatc aggatcgtca ggttcagctt gagctcgggc
    15241 tgcagcacca cctgacgtcc gttgatcaac accgtatcgg gctgatgcga cttgagcacc
    15301 cgctcttcgt cgatgttcac cagcgcggcg ccgagcgact ggtcggcaat cgtgcatttg
    15361 cccgtgtcgt cgaccaacct gccgaaagcg aggctgccct gcggggatcc ggttcgcgcg
    15421 aacaggtacg cgttgaccgc gccggcgagg aacttcaacg ccacgtcgat catgggcgga
    15481 tggctccttc cctcgagccg ggagcgccgt accggcggcg gtactcctcg agcgatcgag
    15541 cgagcacgtc gacgaacgca tcgaccgaac gcggagtggc cacgatgctg gagtgaagga
    15601 cgctgtcggc gtcgccaaac cgctgaccga aatcgaggat gaactcctcg gcactgaagc
    15661 tgacgtcgag ccagttcgcg tatctgcggt gagccgggga ccgtcgctcc tcagtcatgg
    15721 cgcgcagcat cagcaacgaa cgcaccaggc ggaggggcgg cggcgcccgc ggaaactcag
    15781 tggacggagg ccgatgagga agcgtcaggt gtcactcgcg tggcagtttg gccgtgacac
    15841 tgtcacactc ggatgtgaca tcgatgcggt gtcgcgcgag cttgcggtac aacgtcatgc
    15901 gcgaccagtt cagccgctgg gccgcctcgg acttgttgcc gtgcgaggcg cggagcgccg
    15961 cgacgacgcg cacgcgttcg gtttccacgg gcagaggcgt gctgaagtcc gctcctcgta
    16021 cggcgggagg cagcgcctcg atcccgatcg cgtccgactc agtcgtcagg acgctgctct
    16081 cgaccacgtt cctcagctcg cgaacgttgc cgggccattc gtacgccgcc agccgggcga
    16141 ccgcctcgtc cgtgaacgcc gcgccgagac gaccgtacgc gggggcacag tcgcgaagaa
    16201 acgcccgcgc gagcacgggg acgtcgtccg ggcgctcgcg cagcggcggt acgacgaggc
    16261 tgtagacatt gaggcgataa aacagatcgg cgcggaacag tcccgaccgt acgagctctt
    16321 gaagattccg gttcgtggcg gccacccacc gcacgtcgat cgtctcgtgc cggcggctgc
    16381 cgaggcgctg gacctcacgc tgatcgatcg cgcgcagcag cttcggctgc gtcgtcaggc
    16441 tgagctcccc gacctcgtca agcaggacag tgccgccgct ggcggcctgg aggcggccga
    16501 tcgacgtcgc ctgcgcgccg gtgaacgcgc cgcgctcgta gccgaaaagc tcgctctcga
    16561 agagcgtttc gggaagcgcc gagcagttga tcgtgacgaa ggggccctgc gcccgcgggc
    16621 tggactcgtg gatgaggcgg gcgatgaggt ccttgccggt cccggtctcg ccgctgatga
    16681 gcacgctgca cggcgtgacg gcgatgcgtt tgatcaccag ccgcagattc tgcgtgtgcg
    16741 ggctttcgcc gatgagtccg gggacgtccg tgtcgcgcgg ctcgatgtgc aggcggcgga
    16801 ggctctcggc cagccccgtc ggcgtgatcg ggatcctgag gtagtcgttc gctccggatc
    16861 ggaacgcctc gacggcaaga tgctccgacc cggagtaggc gacgaagatc accggactcg
    16921 cccgaaagcg ctggcaaatc tctcgcaggc ccacgagcgg gtgcccgttc gactcgagca
    16981 cgatgacgag gtccgcgcga tcggcacaga gatcggcgcc cagctcgacg agcatgcggg
    17041 caatgcgttc gcgctcgggg gcatcacgcc ccgtgacgaa cacacgcggc gccgccacgc
    17101 ggtcggtcgc actccaatta atcgacaccg caacctcgtg cgtgaccctg tgacgagaac
    17161 gtccgaggga acggtcagtc ttgcgcgccg tgaacgttca cgtcaatcgg gatttcgcga
    17221 agtacttcgt ttttgtgtgc acagacgtaa acgcacgcgc gacgcggcgc gaacaagctc
    17281 agcggctcgg cgtctgcgcc ttcagaatct tggccgtcgt gatcgcctgg cccacgtgcc
    17341 gcgtgcagtg ctcggccgca tggaagagca acccgatcac gttggtgggc agacccgccc
    17401 ggccgagctt gcgttcgtcg aagagcgatt cgcgcggtgt cgcgcgaagc tggtcgagcg
    17461 cgcggtcgat gatcgcgccg acgccggcga cgaccgacgc gagcggcgcg ggcggatcgc
    17521 cggctgcgcc ctcttcacgc agcgccgctc tctgggcgtc cgacagagcc tcgcccctcg
    17581 cgtaggtgaa caggcgatcg agcgcgccgc cggtgtgccg cacgtgaaag ccgatcgagg
    17641 cggcgccgcc gggccgaagc cacacgtgct ccggcggcac atcggaggcg atccgatcga
    17701 gatcctcgcg cgtctggatc agcgcgtgga cgacgggcat caagagagga tcgaaaccgg
    17761 ggacgggtcc tcgttgccag acttcggttt gggaggcggc gctcatgtcc tccactttaa
    17821 cgcacggtcc gggtaccgcg gcgagcaccg gtggacgagg cacaggagcg aggaaagcct
    17881 tcggagcaag ccgtctcgat cgcacgaaaa acgctgcgaa ggccagcgac atggcgtggc
    17941 gatgggggtc cccacgccgc gtccactgga cgttgagccg gagcagcgtt tttccggctt
    18001 gcggagcggc tttcggagcg atgtgcgtcg tccagtcggt gcgattctgt ttcgggatca
    18061 attcggtccg gctgaagccg gacactacgt acgaaacacg aggtccggct gccgccttcg
    18121 cgctgcgcgc tacggcgtcc aagaaagccg gacactacgt acggggcttg tccgcacgca
    18181 ggtgcaccga cacctgcagg atgggcagga cgggacgaat caagccagcc aggaatccgg
    18241 ggagcccctt cctgcgcagg cgctccctca actgacctcc gttcagcccg ggatacacaa
    18301 tccgcggctc cagcccggac tgccggcacc ggcgcagcca cgagccgcgg cggatccacg
    18361 tctcgttgat tccgagctcg cgctccctgc gcccgaatcc gccgccgacg acgaaacgca
    18421 cgagcgacgg cagcggctct ccgcagagat acgccgcacc gcccggtttc agtacgcggg
    18481 cgatctcgcg gaacgtacgc ccgaggtccc acgagtgatg cacgcacgag gccgcgaaca
    18541 cgacgtcggc cgtcgcatcg gcgagcggca gccggttcat gtcagcgaac agccggca
//
LOCUS       DBNGSH010000136         5009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3575631, whole genome shotgun sequence.
ACCESSION   DBNGSH010000136 DBNGSH010000000
VERSION     DBNGSH010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3575631"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1245
                     /locus_tag="AC16CY_03245"
     CDS             235..1245
                     /locus_tag="AC16CY_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007767215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3352821.1"
                     /translation="MRKIDVFNHIFPERYVDRMKDVAPNFKDVGKRVRGVPMLVDLKV
                     RFEIMDTHEDYQQILSIATPPVDAYASPADAADLAQRGNDGMAELVDRYPDRFPGFIA
                     SLPMNDVDAAMRELERCMGALGARGIQICSNVQGKALDAPEFQPIFAAMAAYDLPIWL
                     HPYRAADSADYLTENRSQYEIWWTFGWPYESSVAMARLVFSGIFDRYPALKIITHHMG
                     GMIPYFEGRIGPGWDQLGARTSDRDYTILLKELKKRPFDYFKMFYADTALFGAYEPTV
                     CGLRFFGIDQVLFASDAPFDPQGGRQYIRDTIAVVDRLPISNEERERIYWRNAVRLLK
                     LE"
     gene            1260..2516
                     /locus_tag="AC16CY_03250"
     CDS             1260..2516
                     /locus_tag="AC16CY_03250"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3352822.1"
                     /translation="MARQRAWLFVACVAISTGVAFAGQGPASPRELSRIDRLDAWLSA
                     VEQHRSGTVDGWALGVRDWDAAALDLLRADLGTIVSMIREPKRVVYDGAELARLKAIA
                     QDVVSRGGENRILRRGAMLHADIAMFAPFKNIPSRKPAGGPIRIVNDGQQTDVDRHFQ
                     FGRTLLDSVQVRAARGVRAGPGADETVRLWYLAIGSYLQAFGEMDFRHVERSLQLFPR
                     DAEVLFFAACLQEMASSPQAQSALQAIGPSKGTTSSVRGEGAALREAERLFRQALDRD
                     PLHVETRLRLGRVLGRRGRHEEAARELRQARAATESMLLQYYANMFMGAELEALGRTT
                     EAREAYELAADTYQKAQAPRLALNAMAASAGDRTEALRVIESALRPASIEPDDDPWWD
                     YYTAQARATDVLLGQLRARIAQDGSK"
     gene            complement(2522..3292)
                     /locus_tag="AC16CY_03255"
     CDS             complement(2522..3292)
                     /locus_tag="AC16CY_03255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3352823.1"
                     /translation="MKRREFVAGVAVGAAGVGVAGAGLAGWRRLRSDRPQIVPEFGRL
                     SFSQQGEDIILFHALHDLLGLDRPTYMDVGAAHPVRSSNTYLLYGTGSTGVLVEPNPL
                     FVEMLRRQRPKDVVVAAGIGVTDAQEADYYEIKGNPMLNTFSSEQVARLQKGQTESVV
                     ERVSKMPLLNINRVITEHMGMAPDLLSTDIEGLDYAIIRTLDLTRFRPGVICCEGVSS
                     YKNGNASDIAQYLLSQSYILRGGSLVNSVFVDGNRVDA"
     gene            3468..>5009
                     /locus_tag="AC16CY_03260"
     CDS             3468..>5009
                     /locus_tag="AC16CY_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352824.1"
                     /translation="MHPRRLVFRGLTFYWRTNVAVVAGVAVAVAVLAGALLVGDSVRG
                     SLRELVLGRLGKTDQVVVSSGLFREQLAGDLASDPAFASRFSAAAPLFLARGTVTAQT
                     GGRRAGEVRIYGVDDRFWQFHGVNGVAGPGDREAYISPALAAHLGTAAGDSILVRVQR
                     PTDVPLESLHGRRDNLDRSLRLGVAQVLAQDSLGEFSLEARQGDVLAAFVPLLRLQQE
                     LDIEGFVNTVLVATGLSPPEGPADLEALVRHRAQLDDVGFLVKDVAARGIFVLGSSGG
                     ILDEPHSAAAMKALGGMRAQQVFTYLANNIRVGAREVPYSLVTALDLDALIPNLANVS
                     EDSVVLNAWAAADLRARVGDAVTMEYYVWEEPGQLVTKSSSFRIAGVVPIDAGDRDMA
                     PAYPGITDSPTLDEWDPPFPVDLQRIRPVDEEYWKQYRTTPKAFVTLEAGQRLWRSRY
                     GALTSIRITPDAGRSVSEARDAYVGSLRAMTDPLSLGLTVVDARAQGMGAARGATDFG
                     QYFVYF"
BASE COUNT          815 a         1648 c         1680 g          866 t
ORIGIN      
        1 ctcgggccgc gcgcatggat ggccggcggc ctgctgcgcc gccgcggttt cacgaacgtg
       61 aagtatctgg ccggccactt cagccggtgg cggggcgcgg gacttccaga ggagaattaa
      121 ccccgaactc gcttgtgttt ggtggcgccg cgcccgcctg ttccgagcgg gacggccgtg
      181 gtctcgcatt gttggaagct gaagcttcca acacacgcga gcggtggagt agacgtgcga
      241 aagatcgatg tgttcaacca catcttcccg gagcgatacg tcgaccggat gaaggacgtg
      301 gcgcccaact tcaaggacgt cggcaagcgt gtccgcggcg tgccgatgct ggtcgatttg
      361 aaggtgcgct tcgagatcat ggacacgcac gaggactacc agcagatcct gtcgattgcc
      421 acgccgcccg tcgatgccta tgccagcccg gcggatgccg ccgatctggc gcagcgcggc
      481 aacgacggca tggcggagct ggtggaccgg tatccggatc gcttcccggg gtttatcgcg
      541 tcgctgccga tgaacgacgt tgacgcggcc atgcgggagc tggagcggtg catgggcgcg
      601 ctcggcgcgc gcggcatcca gatctgctcg aacgtgcagg ggaaagcgct cgatgcgccg
      661 gagttccagc cgatattcgc ggccatggcc gcctacgacc tgcccatctg gcttcacccg
      721 taccgcgccg ccgacagcgc cgactacctg acggagaatc gctcgcagta cgagatctgg
      781 tggacgttcg gctggccgta cgaatcgagc gtcgcgatgg cgaggctcgt cttctccggg
      841 atcttcgacc ggtatccggc gctgaaaatc atcacccacc acatgggtgg catgattccg
      901 tacttcgaag gccgcatcgg tcccgggtgg gatcagctcg gcgcgcggac gtcggaccgc
      961 gactacacga ttctcctcaa ggagctgaag aagcggccgt tcgactactt caagatgttc
     1021 tacgccgaca ccgcgctctt cggcgcgtac gaaccgacgg tgtgcgggct tcggttcttc
     1081 ggcatcgacc aggtgctctt cgcctccgac gcgcctttcg atccgcaggg aggccgccag
     1141 tacatccgcg acacgatcgc cgtcgtcgat cgcctgccaa tctcgaacga agaacgagag
     1201 cggatctact ggcgcaacgc cgtgcgtctg ctgaagctcg aatagaatcg ggagcaaccg
     1261 tggcccgaca gcgcgcgtgg ctgttcgttg catgcgtcgc tatctcgact ggcgttgcgt
     1321 tcgccggtca aggacctgcc tcgccgcgcg agctgtcccg gatcgatcgg ctggacgcgt
     1381 ggctgtcggc tgtcgagcag catcgatccg gcacggtgga tggatgggcg cttggcgtgc
     1441 gggactggga cgccgcggcg ctggacctcc tccgggccga cctcgggacc atcgtcagca
     1501 tgattcgcga gccgaagcgg gtcgtctacg acggcgccga gctggcgaga ctgaaggcga
     1561 ttgcacagga cgtcgtgtcg cgcgggggag agaatcgcat cctccggcgc ggcgcgatgc
     1621 tgcatgccga catcgcgatg ttcgcgccgt tcaagaacat cccgtcacgg aagccggccg
     1681 gcggacccat ccggatcgtg aacgacggtc agcagacgga cgtggaccgt cacttccagt
     1741 tcggaaggac gctcctcgac tccgtgcagg ttcgggccgc gcggggagtc agggcaggcc
     1801 cgggcgcgga cgaaacggtg cgcttgtggt acctcgccat cgggtcgtac ctgcaggcgt
     1861 ttggagaaat ggatttccgg cacgtcgagc gatcgctgca gctgtttccg cgggacgcag
     1921 aggtgctgtt cttcgcggcg tgcctgcagg aaatggcgag cagccctcaa gcccagtccg
     1981 ccctgcaggc gattgggcca tcgaagggca cgacctccag tgtccgcggc gagggcgccg
     2041 cgctgcgcga ggccgaacgg ttgttcaggc aagcactcga ccgcgatccg ctacatgtcg
     2101 agactcgact ccggctgggc cgcgtcctcg gccggcgcgg gcgtcacgag gaggctgcgc
     2161 gcgagctgcg acaggcacgt gcggccaccg agagcatgct gctgcagtac tacgcgaaca
     2221 tgttcatggg cgccgagctc gaggccctgg gccggacgac ggaggcgcgc gaggcctacg
     2281 agctggcggc cgacacgtac cagaaggcac aggcccctcg cctggcgctc aacgcaatgg
     2341 cggcgagcgc gggcgaccgt accgaggcgc ttcgggtcat cgaatcggcc ttacgccccg
     2401 cgtcgatcga gccggacgat gacccgtggt gggactacta cacggcacaa gcgcgcgcga
     2461 ccgatgtgct gctcggtcag ttgcgcgccc gcatcgcgca ggacggatcg aagtgacgcc
     2521 ctcacgcgtc cacgcggttc ccatccacga agactgaatt caccaacgag ccgccgcgca
     2581 ggatgtagct ctgagacaag agatactgcg cgatgtcgga cgcgttgccg ttcttgtagc
     2641 tcgacacacc ttcacagcag atcacgcctg ggcgaaatcg cgtcagatcc agagtccgga
     2701 taatcgcgta gtccagcccc tcgatgtcgg tcgatagcag gtcgggggcc atgcccatgt
     2761 gctcggtgat gacccggttg atgttgagca gcggcatctt cgacacgcgc tcgacgacgc
     2821 tctccgtctg gcccttctgc aggcgcgcga cctgctcgga cgagaaggta ttgagcatcg
     2881 ggttgccctt gatctcgtag tagtccgcct cctgtgcgtc ggtgacgccg atgcctgcgg
     2941 ccacgaccac gtccttcggc cgctggcggc gcaacatctc cacgaacagc ggatttggct
     3001 cgaccaggac gcccgtggaa ccggtgccgt acaggagata ggtgttgctg ctgcggacgg
     3061 gatgcgcggc gccgacgtcc atgtacgtcg gccgatcgag cccgagcaga tcgtggagcg
     3121 cgtggaacag gatgatgtcc tcgccctgct gtgagaagct cagtcgcccg aactccggga
     3181 cgatctgcgg ccggtcggat cgaagccgcc gccatccggc cagacccgcg ccagctactc
     3241 ccactcccgc ggcaccgacc gccacgcccg ccacgaattc gcgtcgtttc acaagcccct
     3301 cattgttttg caatgttggc gccgagttga gccggcggtc aacctgaggt taggcgttga
     3361 aaacgcgcct tcgcgtgcgg catgctccat gaaacgtctc gagcgtccca caccgtattg
     3421 ctggcgacgg ccagctcgat gagacgcggc gagcctccct ttccgatatg cacccacgac
     3481 ggctcgtctt ccggggcctg accttctact ggcgcaccaa cgtcgccgtc gtcgccggcg
     3541 tggccgtggc cgtcgcggtg ctcgcgggtg cgcttcttgt aggcgattcc gtccgcggaa
     3601 gcctgcgcga gctcgtgctc gggcgtctcg ggaagacgga ccaggtcgtc gtgtcgagcg
     3661 gcctcttccg cgagcagctc gcgggcgacc ttgcgagcga tccggcgttt gcgagccgct
     3721 tcagtgccgc ggcgccactg ttcctcgcca gagggacagt cacggctcag acaggcgggc
     3781 gccgcgcggg cgaggttcga atttacggcg ttgacgaccg gttctggcaa ttccacggtg
     3841 tgaacggcgt ggcgggtccc ggcgatcgcg aggcatacat cagcccggcg ctcgccgcac
     3901 atctcgggac tgcagcaggg gattcgattc tggtacgcgt acagcgtccg acggacgtgc
     3961 cgctcgagtc gctgcacggc cggcgcgaca accttgaccg ctcgcttcgc ctcggcgttg
     4021 cacaggtcct ggcacaggac tcgctcggcg agttttcgct cgaggcccgg caaggcgacg
     4081 tcctcgccgc tttcgtgccg ctgttgcgat tgcagcagga gctcgacatc gagggcttcg
     4141 tcaacaccgt gctcgtcgcc acgggcctga gcccacccga gggtccagcc gatctcgagg
     4201 ccctcgttcg tcaccgcgcg cagcttgacg acgtcggttt cctggtcaag gatgtcgccg
     4261 cgcgcggcat cttcgtcctc ggctcgagcg gcggcatcct cgacgagccg cactcggcgg
     4321 ccgcgatgaa agcgctcggc ggcatgcggg cgcagcaggt cttcacgtat ctcgccaaca
     4381 acattcgagt gggcgcccgc gaggtcccgt actcgctcgt caccgcgctc gatctcgacg
     4441 cgctgattcc gaacctcgcc aacgtctccg aagactccgt cgtcctgaac gcgtgggccg
     4501 ccgccgatct gcgagcgcgc gttggcgacg ctgtgacaat ggagtactac gtctgggagg
     4561 agccgggcca gctcgtcacg aaatcctcca gtttccgaat cgcgggcgtt gttccaattg
     4621 atgccgggga tcgcgacatg gcgccggcct accccggtat caccgattcg ccgacgctcg
     4681 acgagtggga tccgcccttt ccagtggatc tccaacggat ccgccccgtg gacgaagagt
     4741 actggaagca gtaccgaacc acgccgaagg cgttcgtaac gctcgaagcg gggcaacggc
     4801 tctggcggtc gcgctacggc gcgctgacgt cgattcggat cacgccggac gcgggccggt
     4861 ccgtcagcga ggcccgcgac gcgtacgtcg gcagcctgcg cgccatgacc gatccgctgt
     4921 ctctggggtt gaccgtcgtt gacgcacgcg cgcaggggat gggcgccgcc cgcggcgcga
     4981 cggacttcgg ccagtatttc gtctacttc
//
LOCUS       DBNGSH010000137         3602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_179711, whole genome shotgun sequence.
ACCESSION   DBNGSH010000137 DBNGSH010000000
VERSION     DBNGSH010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_179711"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="AC16CY_03265"
     CDS             complement(<1..1169)
                     /locus_tag="AC16CY_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3352825.1"
                     /translation="MATALTRRDTLKRGLAAASLLALPEWVIPALAQGEADLPFTDIP
                     ANFNPIDPNGNRRSLDLRKIDGFLTPVDQFFFIQHYNKPEIDPAKYRLKVSGLVDRPA
                     ELSLDEIRGMRPTEIVNGYECSGNSPRAMQGLTSNGRFTGVRLRDVLRRVGIGEKARE
                     VVFFGADRGPEEVVFRQQTFKIQQQFARSVTLENATKDEPLLAYALNGQPLDRDHGAP
                     LRLIMPGWYGVANVKWLAEIRLQEGRFLGNYQARWYRTMRTEGGSGTETDPETQWVET
                     EVTRLNLKSVIARVRKSSGAHQVFGFVLNDGTPLKSVEVQIDGGPWRAATLDAANTKH
                     SWKLFTYRWEGATAGEHTLVSRATDVEGRVQPTAAELARKRTFLEDNSQFPRKVMV"
     gene            complement(1233..1391)
                     /locus_tag="AC16CY_03270"
     CDS             complement(1233..1391)
                     /locus_tag="AC16CY_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352826.1"
                     /translation="MRKALPGIVVAALLAVSMSDARPFGIDAWKIVLALFGLVLFVAA
                     GPGGRRRT"
     gene            1458..3221
                     /locus_tag="AC16CY_03275"
     CDS             1458..3221
                     /locus_tag="AC16CY_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR3352827.1"
                     /translation="MAGPDDQRPGAAGTPVPDLIERYRSRRDAWIAQADELARLRDEV
                     RGSAEREAMEIVTAARRDVRRIVTEARRELLVLSAQVQAALGEAAAKADPTTLLQKAG
                     ITGGEDTTRFSLPPSSEFGAESGESGVEDILKDMQADVTALAEGVRALPIQAESPPRI
                     GNGSAEPAPPPPRLVAPTQSAPVSRPTQTPADEERTPSAAALAQAASRALLSSPYPGD
                     AVPVVPVRRVRAFVGASVAAGVLILLGTIWWLRGPAEPVEPARSVAADTATPPKEPKD
                     TPAVSAPAAAASSPSEQALPVRNAAATRSGNLSVVVESLRDVWVRTTIDGREDQGRTF
                     SAGEVVNVSADQSISLRVGDAGALVASVNGGEKRPLGRDGQVVTRQFVVEDLRNAAVR
                     KPSSPAAPPPAAAAAQTVRSNPSGTGPVLPPLPEPAPSVPAAAAPQPVATGNASSANP
                     PAAAAALPSSPASASVESQSAQRAVVAAARQWLDAYYRQDRAAMAALSTENVTLADER
                     RPDERLPSGLADVKRSLDSPSVQIAAETAVLTAVMTEQSATLPAPRVSPVSQVWVLSG
                     GQWKVRQTRFVSEARLNQIFR"
     gene            3303..>3602
                     /locus_tag="AC16CY_03280"
     CDS             3303..>3602
                     /locus_tag="AC16CY_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352828.1"
                     /translation="MSSLDEQVRSALDRVVESLKGQLESDLTTSAADILRAVAEDRDR
                     ADADRQQETDQQLAALRQEFERERAEFQRQTADEIAALKQTIESVRSELGTAALAT"
BASE COUNT          583 a         1229 c         1190 g          600 t
ORIGIN      
        1 accatcacct ttcgcgggaa ctgcgagttg tcttccagga aggtcctctt tctggccagt
       61 tccgcggccg tgggctgtac gcggccttcg acgtcggtcg cccgcgacac gagcgtgtgc
      121 tcgccagccg tcgcgccttc ccagcggtac gtgaacagct tccaggagtg cttcgtgttg
      181 gcggcatcga gcgtggccgc gcgccagggg ccgccatcga tttgcacttc gacagacttg
      241 agcggcgtgc cgtcgttcag cacgaacccg aacacctggt gggcgccact ggatttcctc
      301 acgcgcgcga tcactgactt caggttcaac cgggtcacct cggtctccac ccactgcgtt
      361 tcgggatccg tctcggtgcc gctgccgccc tcggtccgca tcgtccggta ccagcgcgcc
      421 tggtagttgc cgaggaagcg gccttcctgc aggcggatct ccgcgagcca cttcacgttg
      481 gctacgccgt accaaccggg catgatcagc cggagcggtg ccccgtggtc gcggtcgagc
      541 ggctggccgt tgagcgcgta ggccagcagc ggttcgtcct tcgtggcgtt ttcgagcgtg
      601 acgctccgcg cgaactgctg ctggatcttg aacgtctgct gccggaacac aacctcctcg
      661 ggcccgcgat cggccccgaa gaacaccacc tcgcgcgcct tctcgccgat gcccacccgg
      721 cgcagcacgt cgcgcagcct gactccggtg aaccgtccgt tggacgtgag gccctgcatg
      781 gcacgggggc tgttgcccga gcactcgtat ccgttgacga tctcagtcgg gcgcatgcca
      841 cggatctcgt cgagcgacag ttcggcgggc cggtccacca gccccgacac cttgagccgg
      901 tatttcgccg gatcgatctc cggtttgttg tagtgctgga tgaagaagaa ctgatcgacc
      961 ggcgtcagga acccgtcgat ctttcgcaag tcgagcgatc gccggttgcc gttcggatcg
     1021 atcgggttga agttggccgg gatgtcggtg aaaggcagat cggcctcgcc ctgtgccagg
     1081 gcagggatca cccattcggg aagcgcgagc aggctcgcgg cggccagtcc tcgtttcagc
     1141 gtgtctcggc gggtgagtgc agtcgccatc gtcgatcctt tcgtaacggg ccgggtgcga
     1201 aggtgcgaag gacgatgtga atgataccgc catcaggtgc ggcggcgccc gcccggtccg
     1261 gccgccacga acaacacgag gccgaacaag gcgaggacga tcttccacgc gtcgataccg
     1321 aagggcctcg cgtcgctcat gctcaccgcc agaagggcgg cgacgacgat cccggggagc
     1381 gccttgcgca tcggactgag cctctgagac aaattctggc tcgactttcc tacgatccgg
     1441 ccgcaaaatg ctagtccatg gctggaccag acgaccagcg gcccggcgcg gcaggcactc
     1501 cagtcccgga cctcatcgaa cgctaccgct cgagacgcga cgcgtggatc gcccaggcgg
     1561 acgagctggc gcgtctgcgc gacgaggtgc gcggctcggc cgagcgggag gcgatggaga
     1621 tcgtgacggc cgcgcgccgc gacgtgcgtc gaatcgtcac cgaggcgcgc cgcgagcttc
     1681 tcgtcctgtc cgcgcaggtt caagccgccc tgggtgaagc agcagccaag gccgatccca
     1741 cgacgttgct tcaaaaggcc ggcatcaccg gcggcgaaga cacgacccgg ttctcacttc
     1801 cgcccagcag cgagttcgga gccgagtccg gtgaatcggg tgtcgaggac atcctgaagg
     1861 acatgcaggc cgacgtgacc gcgcttgccg agggcgtgcg cgcgctgccc attcaggcgg
     1921 agtctccgcc tcgcattggc aatgggtccg cagagcctgc acctccgcct ccccggctgg
     1981 ttgcaccaac tcagtcggcg ccagtgtcga gaccgactca gacgccagcc gacgaggagc
     2041 ggacaccgag cgcggccgcg ttggcccaag ccgcgtcccg ggcgttgctg tcttctccgt
     2101 atccgggcga tgccgtgccc gtcgtgccgg ttcgacgagt cagagcgttt gtgggcgcct
     2161 cggtggcggc cggcgtgctg atcctcctgg gcacgatctg gtggctgcga gggcctgccg
     2221 agccggtcga gcccgcaagg agcgtcgcgg cggacaccgc gacgccgccg aaagagccga
     2281 aagatacgcc cgctgtatcg gctcccgcgg cggcggcctc gtcgccttcg gaacaggcgc
     2341 tgccggtgcg caacgcggcg gccacgcggt cgggcaatct gtcggtcgtc gtcgaatcgc
     2401 tccgcgacgt ctgggtgcgg accaccatcg acggacgcga ggaccaggga cgtacgtttt
     2461 cggcgggtga ggtcgtcaac gtgtcggcgg atcagagcat ttcgcttcga gtgggcgacg
     2521 ccggcgccct ggtcgcctcc gtcaacggcg gcgagaagcg tccgctcggg cgggacgggc
     2581 aggtcgtgac acgtcagttc gtcgtggagg acctgcgcaa cgcggccgtt cgaaagccat
     2641 cgagccccgc cgcgccgccg ccggcagcag cggctgcgca gactgtcaga tcgaacccgt
     2701 ccggtacggg accagtgctt ccgccattgc cggagccggc cccgtcggtt ccggccgcgg
     2761 ccgctcctca gcccgtcgca acaggtaacg cctcatctgc caatcctccc gctgcggctg
     2821 cggcgctgcc atcgtcgccc gcttcggcat cagtcgaatc ccagtcggcg cagcgagcgg
     2881 tggttgcagc ggcgcgacaa tggctcgacg cctattacag gcaagacagg gcggccatgg
     2941 ccgcgctttc gaccgagaac gtgacgctcg ccgacgagcg gcgccccgat gagcgtcttc
     3001 cctccggcct tgccgacgtg aagagatcgc tcgacagccc gagcgtgcag atcgcggctg
     3061 agacggcggt attgaccgcc gtcatgacgg agcagtcggc gacgctgcca gcacccagag
     3121 tgtctccggt ctctcaagtg tgggtgctgt ccggcggcca gtggaaagtc cggcaaacac
     3181 gtttcgtcag cgaagcacgg ctgaaccaga tcttccgcta gctcgtccta ttcccccagg
     3241 cgcggcaagc gccccgcact ctcgcacctt cgtcccctcg gacccttata atcagcacaa
     3301 ctgtgtcctc gcttgacgaa caagtcagat cggctctcga ccgcgtcgtc gaatccctca
     3361 agggccagct cgagtccgac ctcacgacgt cagccgctga catcctgcgc gccgtggctg
     3421 aggatcgcga tcgggcagac gccgatcggc agcaggaaac ggatcaacaa ctcgccgcgc
     3481 ttcgtcagga attcgaacgt gaacgggccg agtttcagcg ccagaccgcg gatgaaatcg
     3541 ccgcgctgaa acagacgatc gagtcggttc gttcagagct tggcaccgcc gccctcgcga
     3601 ca
//
LOCUS       DBNGSH010000138         3109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_519723, whole genome shotgun sequence.
ACCESSION   DBNGSH010000138 DBNGSH010000000
VERSION     DBNGSH010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_519723"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="AC16CY_03285"
     CDS             <1..630
                     /locus_tag="AC16CY_03285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3352829.1"
                     /translation="RRELAQMMITTRESTLREISRELHDEFGQILTAIGSMLGRAGKQ
                     APEGSSLRNDVREIAEVAQTALDGVRGLSQTLHPSILEELGLAATIDWYISTVEKQLG
                     ITVALEQAGSPAPVDSTRAIHVYRILQEALSNVARHSGTTRAVVRLRFDTGKLDLEVE
                     DQGRGLDLGTARRGLGLVAMRERASLVGGTLEFLRPARGGTRVRFSMPL"
     gene            627..1256
                     /locus_tag="AC16CY_03290"
     CDS             627..1256
                     /locus_tag="AC16CY_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3352830.1"
                     /translation="MSERITVLLADDHALVRRGFRRILEDDPSISVVGEAGNGDEAVR
                     MFEELRPDVVVMDAAMPGSSGLTATREILKAAPKAAILMLSMHSEETLVRQAMAAGAR
                     GYILKNAIDLDLADAVRRVAAGETVLDPEVVRPEPLAGERTRRLTPRETEVLQLICNG
                     LSNREIAARLDLSVNTVAVHRANIMNALGVHKTAELVVYALQHGLVNPL"
     gene            1253..2938
                     /locus_tag="AC16CY_03295"
     CDS             1253..2938
                     /locus_tag="AC16CY_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3352831.1"
                     /translation="MNRRDFLRVAGSGAAASMLPLQALAQPPLAFRLVDVTASAGLRF
                     RHTSGAYGGKLLPETMGSGCAFLDYDADGWPDILLVNGRDWPGHVRQRSTLHLYRNNR
                     NGTFTDVTRAAGLDVEIYGMGVAVGDYDNDGFPDVLVTAVGQNRLFHNTGKGTFVDVT
                     RSSGLGGREAFSTSAMWVDFDRDGLLDLFVCNYVRWSAEHDVFCSLDGKQKSYCTPEA
                     YRGETSWLFRNRGNGMFEDVTATCGIFNSSAKSLGVAIVDDDQDGWPDIFVANDTQPN
                     KLYRNLRNGRFRDVALETGVALSADGKARAGMGVDSGDFDNSGRMGLAVTNFDNEMIG
                     LYRPQASGAYADIATRSGVGAASLNRLGFGCLFADLDLDGALDLVVANGHIDDTVRHI
                     RGNVGYAQAPLLFLNQGNGSFRDVAPQAGSPFATPRVGRGLACGDFDRDGDVDLLMTT
                     NNGPAVLFRNDQTGGRRSVRFRLRGTESNRDAIGATVRVFHGGTGQTRMVKSGSSYLS
                     QSELPVTFGVGTRDVIDRVTIAWPNGRKEEFTKVATGKQYDCTEGKGLTESSR"
BASE COUNT          526 a         1045 c         1052 g          486 t
ORIGIN      
        1 cggcgcgagc tggcgcagat gatgatcacg acccgcgagt ccaccttgcg cgagatctcg
       61 cgcgagctgc acgacgagtt cggccagatc ctcaccgcga tcggctcgat gctcggcagg
      121 gccggcaagc aggcccccga ggggtcgtct ttgcgcaacg acgttcggga gatcgccgag
      181 gtggcgcaga cggcgctcga cggcgtgcgc gggctgtcgc agacgttgca cccctcgatt
      241 ctcgaggagc tgggcctggc cgccacgatc gactggtaca tctcgaccgt cgagaagcag
      301 ctcgggatca ccgtcgctct cgaacaggcc ggctcgccgg cgccggtcga ttcgaccagg
      361 gcgatccacg tgtaccgcat cctgcaggaa gcgttgagca acgtcgcccg ccattcagga
      421 accacccgcg ccgtcgtgcg cctgcggttc gacacgggta aactggacct cgaagtcgaa
      481 gaccagggcc gcggcctcga cctcggcacc gcgcgccgcg ggctggggct ggtggccatg
      541 cgggagcgcg cctccctcgt cggcggcacg ctggagttcc tgcgtccggc gcgcggcggc
      601 acgcgcgtcc gattctcgat gcctctatga gcgaacgcat caccgtgctg cttgccgacg
      661 atcacgcgct cgtgcggcgc ggcttccggc gcatcctcga agacgacccc tcgatctccg
      721 tcgtcggcga agccggtaac ggcgacgagg cggtccgcat gttcgaggag cttcggccgg
      781 acgtggtcgt gatggacgcc gcgatgccgg ggtcgagcgg cctgacggcg acgcgcgaga
      841 tcctgaaagc cgcgccgaag gccgcgatcc tcatgctcag catgcactcc gaggagacgc
      901 tcgtgcgcca ggcgatggcc gcaggcgcgc gcggctacat cctcaagaac gccatcgatc
      961 tcgatctcgc ggacgccgtg aggcgcgtcg cggccggcga aacggtcctc gacccagagg
     1021 tcgtacggcc cgagccgctg gcgggcgagc gaacgcggcg cctgacgccg agagagacgg
     1081 aggtccttca gctgatctgc aacggtctct cgaaccgtga gatcgcggca aggctggacc
     1141 tcagtgtcaa caccgttgcc gtgcaccgcg ccaacatcat gaacgcgctc ggcgtccaca
     1201 agacagcgga gctcgtcgtc tacgcgctgc agcacgggct cgtgaatcct ttgtgaaccg
     1261 ccgggacttc ctgcgcgtcg ccggttcggg cgccgcagcc tcgatgctgc cgcttcaggc
     1321 gctcgcacag ccgccgctcg cgttccggct cgtggacgtc actgcgtccg ccgggctgcg
     1381 attccgtcac acgagcggcg cgtacggagg caagctcctt cccgagacga tgggctcggg
     1441 gtgcgcgttt ctggattacg acgcggacgg ctggcccgac atcctgctcg tcaacggcag
     1501 ggactggccc ggccacgtca ggcagcgctc gacgcttcac ctgtacagga acaaccgcaa
     1561 cgggaccttt accgacgtga cccgggccgc gggcctcgac gtcgagatct acgggatggg
     1621 cgtcgccgtc ggcgactacg acaacgacgg cttccccgac gtcctcgtca cggcggtcgg
     1681 ccagaaccgg ctctttcaca acaccggcaa ggggaccttc gtagatgtca cgcgctcgag
     1741 cggcctcggc gggcgcgagg cgttcagcac gtcggcgatg tgggtcgatt tcgatcgcga
     1801 cggactgctc gacctgttcg tctgcaatta cgtgcgctgg tcggccgagc acgacgtctt
     1861 ctgcagcctc gacgggaaac agaagtccta ttgcacgccg gaggcgtacc gcggcgagac
     1921 gagctggctg ttccggaacc gcggcaacgg catgttcgaa gacgtcacgg cgacgtgcgg
     1981 catcttcaac tcgagcgcca agtcgctcgg cgtggcgatc gtggacgacg accaggacgg
     2041 ctggcccgac atcttcgtgg ccaacgacac gcagcccaac aagctgtacc gcaacctgcg
     2101 caacggccgg ttcagggacg tcgcgctcga aaccggcgtg gcgttgagcg ccgatggcaa
     2161 ggcacgcgca gggatgggtg ttgactcggg cgacttcgac aactccggac ggatgggcct
     2221 tgcggtcacc aacttcgaca acgagatgat cgggctctac cggccgcagg cgtcgggtgc
     2281 gtacgccgac atcgccaccc gctccggcgt cggcgcggcc tcgctcaacc gcctcggctt
     2341 cgggtgcctc ttcgccgacc tcgatctcga cggcgcgctc gacctggtcg tcgccaacgg
     2401 ccacatcgac gacacagtcc gacacattcg gggcaacgtc ggctacgcgc aggcgccgct
     2461 cctcttcctg aaccagggca acgggtcgtt ccgcgacgtc gcgccgcagg ccggcagccc
     2521 gttcgccacg ccgcgcgtgg gccgcggcct cgcgtgcggc gacttcgatc gcgacgggga
     2581 cgtcgatctc ctgatgacga ccaacaacgg ccccgccgtc ctctttcgca acgatcagac
     2641 cggagggcgg cgcagcgtga ggttccggct gcgcgggacc gagtccaacc gcgacgccat
     2701 cggcgccacc gtgcgcgtct tccacggcgg caccggtcag acgaggatgg tcaagagcgg
     2761 gtcgagctac ctgtcgcagt ccgagcttcc ggtgaccttc ggcgtcggca cgcgggatgt
     2821 catcgaccgc gtgaccatcg cctggccgaa tgggcggaag gaggagttca cgaaggtggc
     2881 gacggggaag cagtacgact gtacggaagg caaagggctc acggagagct ctcgatgact
     2941 cccaactccc aaaatgacct ggcattgcgg ctgcatgtcg tgatgtgagc gtctgactgg
     3001 atactctgaa aacgttcgat ggtgcagttt ctcagcaagc gaggaccaaa aggggcggca
     3061 atttcgtatc agttgctgcg tggaagttgg agactggagc tgacatgaa
//
LOCUS       DBNGSH010000139         6674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2217364, whole genome shotgun sequence.
ACCESSION   DBNGSH010000139 DBNGSH010000000
VERSION     DBNGSH010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2217364"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="AC16CY_03300"
     CDS             <1..1227
                     /locus_tag="AC16CY_03300"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3352832.1"
                     /translation="LPTTAGSDALAKNVTGRDAPIIARLREAGVVVLGKANLSEWANF
                     RSTKSTSGWSAVGGLTRNPYALDRNACGSSSGSGVAVAASLAAMAVGTETDGSVTCPA
                     AVNGIVGLKPTVGLLSRRYIVPITSAQDTAGPMTRAVADAAALLSAMAGSDSGDAATR
                     EADARRTDYPAALSAGALAGKRLGVMRFSMGYLPALDAVFDKAVDQIKAAGADVVTID
                     TFAGMDTIDRQELTILLTDFRKELNAYLASTPPSVTTRTLKDLIAFNSANAAREMPHF
                     GQELFEQSEQTSGHDAGAYGRMREATKAAGRTGIDTMLKAHKLDALIAPTLSPAWTTD
                     LVNGDHFLGGATTLAAVSGYPHLTVPMGFVRGLPVGLSFIGTAWSEAALLGMGYAFEQ
                     RTNARQPPAYLPRIEP"
     gene            1337..2659
                     /locus_tag="AC16CY_03305"
     CDS             1337..2659
                     /locus_tag="AC16CY_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR3352833.1"
                     /translation="MDRRSFLKSAAASAAAAVPFTAFIARAEARVKPVGVGYGPLAPT
                     LDQTTGLPLLHLPEGFSYVSLGWTGDLMINGLPTPSAHDGMAAFNGGGHKVKLVRNHE
                     QGPGSPFSSAFFDAGASGGTTTLVFDSKEGQVIETADSVSGTIRNCAGGPTPWGSWMT
                     CEETTITVNEPHGYIFDVPADGLSNATPIRDMGRFSHEALAVDPVSGYIYETEDAGNS
                     SGFYRYVPNKRYRPGEGGSLFMLKVQRANLANLGASYPNGATFDVEWVPIESPDDTSS
                     TMPSNFVWAQGRTQGAATFARLEGCWYGNDKKIYVVSTSGGTGQGQIWEYDPKAETIM
                     LLFQSPSAAILNAPDNITVSPRGGLVLCEDGSGEEFLHGLTVNGEIFQFAKNSAVLNG
                     ERNGIVGNFSGSEFAGACYSPDGKWLFANIQSPGITFAITGPWETGAL"
     gene            2721..3140
                     /locus_tag="AC16CY_03310"
     CDS             2721..3140
                     /locus_tag="AC16CY_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3352834.1"
                     /translation="MLFTIQFKSGKPVYLQLVDQVKAAAASGAVRPGEALPSIRPLAE
                     ELRVNRNTVAKAYAELETQGVIETMAGKGCFVRPNNSPLKKDVRRKVLAEAIDEAVVQ
                     AHHFQVARAEFVRLAEDRFDALEQRRARAATTNTGRS"
     gene            3137..4042
                     /locus_tag="AC16CY_03315"
     CDS             3137..4042
                     /locus_tag="AC16CY_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3352835.1"
                     /translation="MSSPDMPAIEITDLVRRYGRTEAVNGLTLRVPAGRCYGLFGRNG
                     AGKTTTIKCLLNLLRPTSGSVRVFGLDPVRDEVAVKTRLAYVPDAVAFYPWMTVRHTL
                     DYFASFRSRWNTAVEGALIDQFRLDPHQKTSHLSKGQRTQLALVTAVCADPDLLVLDE
                     PTSGLDPIVRREFIRTVIGAYQDADPGRTVFVSTHLISEFEGLIDRFTIIDHGRDVLT
                     LDADAARERYQRIYARFPEEPRALDLTAAHVIRREGREIEFVARGNSMELLERVMAYS
                     PETVKTEALSLEDIFVSAHAAGAAA"
     gene            4039..5568
                     /locus_tag="AC16CY_03320"
     CDS             4039..5568
                     /locus_tag="AC16CY_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352836.1"
                     /translation="MTTTRARLLVVKELRGLLPLWLATAATMAAAAVYPRSEFLVLGL
                     CAFPLGTVSLGAFSVGQEYSYRTLPVLLAQPIPRYRLLLAKAAALVPLIVLLAILARI
                     VFLRAGRAGMTSADDWLTAAFALVPVMGVCIAPWLTMVFRSVIAGVVFTIAIPSALWL
                     LINVVPAVIRGVDAPYDYSLYLTIFGAGTLAAGAIALVDGPRRFFKLEVTQDAWEIPL
                     SGARRHRALSAAETGRHRNPLVHLVAKELRLQRAAFLLPAFYALAWAALVAARSNFSL
                     GAAFFGMTLIYCGVTAMLVGALASAEERALGTLAVQALQPLAAWKQWLVKVAIVLALT
                     VTFTVLLPSVLEWAHPLSQGEAGWRFFGAFLALMASSLYISSLSTGALQAMLLSMPFA
                     AVAGWLFVVTTSQTSKLIWVPLSRVFRTLSVDWTRPIPLLSTADSDWYIWSMRWLQVA
                     VLCVLVALLTWMAARNHRSAEHGLGRMRRQLLWLVPCVVMGGVISGAVPPLLLWFLGT
                     H"
     gene            5633..>6674
                     /locus_tag="AC16CY_03325"
     CDS             5633..>6674
                     /locus_tag="AC16CY_03325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3352837.1"
                     /translation="MSKAVRDLSASFSGRLLLSGDAGYEEARRVHNGLIDKRPAVIAQ
                     CRGTADISDAVAFARTHGLRISIRGGGHNVAGRAVVDAGMMIDLSLMRSVVVDRASRT
                     ARVEGGATWKEVNRETQQFGLATTGGVVGTTGVAGLTLGGGFGWLMARHGMSLDNLRS
                     VDIVLANGTSVRASATDHADLFWAVRGGGGNFGVVSSFEFTLHDIGPTIAGGLVAWPM
                     AAARDVLRFFRDYTASIGDDVFTVCALLTGPDGATKLAAIAAAYLGPATGAEAALAPV
                     KRFGSPVMDAMGPISYVALNGLLDGAFPKGALNYWKAPFLDRMEDRAIDAAVTAMASC
                     PSPMSQILFEHFH"
BASE COUNT         1055 a         2249 c         2211 g         1159 t
ORIGIN      
        1 cttccgacga cggcgggatc ggacgccctt gcgaagaacg tgacggggcg ggacgcgcca
       61 atcatcgcca ggctgcgcga ggccggtgtc gtcgtgctcg gcaaggcaaa cctgagcgag
      121 tgggcgaatt tcaggtccac caagtccacc agcggatgga gcgccgtcgg cggactgacg
      181 cggaacccgt acgcgctcga tcgaaatgcc tgtggatcga gctcgggcag cggcgtcgcc
      241 gtggctgcca gcctcgcggc gatggcggtc ggcaccgaga ccgatgggtc ggtgacgtgt
      301 cccgcagcgg tcaacggcat cgtcggtctc aagccgacag tgggactgct gtcgcggcgg
      361 tacatcgttc ccatcacgtc ggcgcaggac accgcgggcc cgatgactcg agcggtggcc
      421 gacgcggcgg cactgctgtc agccatggct gggagcgatt cgggcgacgc ggcgacaagg
      481 gaggcggatg cccgccggac tgactacccg gcagcgttgt cggccggtgc cctcgccggc
      541 aagcgcctgg gcgtgatgcg cttctcgatg ggctatcttc cggccctcga cgccgtcttc
      601 gacaaggccg tggatcagat caaagctgcg ggcgcggacg tggtgaccat cgacacgttc
      661 gccgggatgg acaccatcga cagacaggag ttgacgattc tgctcaccga tttcaggaag
      721 gagttgaacg cgtatcttgc gtcaacgccc ccttcggtga cgacgcgcac gctgaaggat
      781 ctcatcgcat tcaactcggc caacgcggcg cgcgagatgc cgcatttcgg acaggagctg
      841 ttcgagcagt cggagcagac ctccgggcac gacgctggcg cgtacggaag gatgcgcgaa
      901 gcgacgaaag cggccggtcg taccgggatc gacacgatgc tcaaggcgca caagctcgat
      961 gccctgattg cgccaacgct cagcccggcg tggacgacgg acctcgtcaa cggggatcat
     1021 tttctgggcg gcgccacgac cctggccgcc gtttcaggct atccgcacct gaccgtcccg
     1081 atgggcttcg tccgtggtct ccccgtgggt ctgtccttca tcgggaccgc gtggtccgag
     1141 gccgcgctgc tcggcatggg gtacgccttc gagcagcgga ccaacgcgcg ccagccgccg
     1201 gcctatctgc cgcgcatcga accgtaacaa ctctggatcc tcttctttac ctcgctgtcg
     1261 actgtttgag acatcgactc gcgacactcc ttggtgcgag cgcgcctccc cgtcgcgccg
     1321 caacgaggag gtctcgatgg atcgacgttc attcttgaaa agcgctgccg cctcggccgc
     1381 agcggctgtg ccgtttaccg ccttcatcgc tcgggccgag gccagggtca agccggtcgg
     1441 cgtcggttac gggccgctcg cgcccacgct ggatcagacg acggggctac ccctgctgca
     1501 ccttccggag gggttctcct atgtgagtct gggctggacc ggcgacctca tgatcaacgg
     1561 gctgcccacg ccgtcggcac acgacgggat ggccgcgttc aacggcggcg gtcacaaggt
     1621 gaagctcgtg cggaaccacg aacaaggccc cggctccccc ttcagctccg ccttcttcga
     1681 cgccggcgcc tcgggcggca cgacgacgct cgtcttcgat tcgaaggaag gccaagtgat
     1741 cgagactgcc gacagcgtca gcggcacgat ccggaactgc gccggcggcc ccacgccctg
     1801 gggcagctgg atgacgtgcg aggagaccac catcaccgtg aacgagccgc acgggtacat
     1861 cttcgacgtg cccgccgatg ggctgagcaa cgcgacgccg attcgcgaca tgggccgttt
     1921 ctcgcacgag gcgctggctg tcgatcccgt ctcgggctac atctacgaga ccgaagacgc
     1981 cggcaacagc tcgggcttct accgctacgt tcccaacaag cggtaccgac ccggcgaggg
     2041 tggctcgcta ttcatgctga aggttcagcg cgccaacctg gcgaacctgg gcgcaagcta
     2101 tccgaacggc gccaccttcg acgtcgagtg ggttcccatc gagtcgcccg acgacacgtc
     2161 gtccaccatg ccgtcgaact tcgtctgggc acaggggcgg acgcagggcg cggcgacctt
     2221 cgcgcggctc gaagggtgct ggtacggcaa cgacaagaag atctacgtcg tctcgaccag
     2281 cggcggcacc gggcagggcc agatctggga gtacgacccg aaggccgaaa ccatcatgct
     2341 gctgttccag tcgcccagcg cggcgatcct gaacgcgccg gacaacatca cggtcagtcc
     2401 gcgcggcggg ctcgtgctgt gcgaagacgg cagcggcgag gagttcctcc acgggctgac
     2461 cgtcaacggc gagatcttcc agttcgccaa gaacagcgcc gtcctcaacg gcgaacgcaa
     2521 cggcatcgtc ggcaacttca gcgggtccga gttcgccggc gcgtgctaca gccccgacgg
     2581 gaaatggctg ttcgccaaca tccagagccc gggcatcacc ttcgccatca cggggccctg
     2641 ggagacgggt gccctgtagc ggcgtcgtcg cttgacagga ggccgcccac tgtcctaatg
     2701 tcataggaca gttggcggcg atgctgttta ccatccagtt caagtcgggc aagcccgtct
     2761 acctgcagct cgtcgatcag gtcaaggcgg ctgccgcctc cggcgccgtt cgccctggcg
     2821 aggcgctgcc gtccatacgc ccgctcgccg aggagctccg cgtcaaccgg aacaccgtgg
     2881 ccaaggcgta cgccgagctc gagacgcagg gcgtgatcga gacgatggcg ggcaaggggt
     2941 gcttcgtgcg cccgaacaac tccccgctga agaaggacgt tcgccggaag gtgctcgccg
     3001 aggcgatcga cgaggctgtg gttcaggctc accacttcca ggtcgcgcgc gcggagttcg
     3061 tccggctcgc cgaggatcga ttcgacgcac tcgagcagcg ccgggcgcgc gccgcaacca
     3121 ccaacacagg acgatcatga gctcaccaga catgccggca atcgagatca ccgatctggt
     3181 ccgccgatac ggccgcaccg aggcggtcaa cgggctgacg ctgcgcgtgc ccgccggacg
     3241 gtgctacggc ctgttcggcc gcaacggcgc cggcaagacg acgacgatca agtgcctgct
     3301 gaacctgctc cgccctacca gcggttcggt gcgcgtcttc gggctcgacc cggtgcgcga
     3361 cgaagtcgcc gtgaagacgc gcctcgccta cgtgccagac gccgtggcgt tctacccctg
     3421 gatgaccgtt cggcacaccc tcgactactt cgcctcgttc cgatcccgct ggaacactgc
     3481 cgtcgagggc gcgctaatcg accagttccg tctcgatccg catcagaaga cgagccacct
     3541 gtcgaagggt cagcgcaccc agctggcgct cgtcacggcc gtgtgcgcgg atccggatct
     3601 cctggtcctg gacgagccga catcgggtct cgatcccatc gtccggcgcg agttcattcg
     3661 aaccgtgatc ggcgcctacc aggacgccga cccgggccgc acggtgttcg tctcgactca
     3721 cctcatctcc gagttcgagg ggctcatcga tcggttcacg atcatcgacc acggccggga
     3781 cgtcctcacg ctcgacgccg acgcggctcg cgaacgctac cagcggatct acgcgcgctt
     3841 cccggaggag cccagggcgc tcgatctgac cgccgcgcac gtcatccggc gcgaggggcg
     3901 cgagatcgag ttcgtcgcgc gcggcaattc gatggagctg ctcgaacggg tgatggcgta
     3961 ctctcccgaa accgtgaaga ccgaggcgct ctcgctcgag gacatcttcg tctcggcgca
     4021 tgcagcggga gccgccgcat gacgaccacg cgggccaggc tgctcgtggt gaaggagctc
     4081 cgcggactgc tgcccttatg gctcgccacg gccgccacga tggccgccgc cgcggtctac
     4141 cccagaagcg agtttttggt gctcggactg tgtgccttcc cgctcggcac agtgtcgctc
     4201 ggcgcctttt cggtgggaca ggagtattcg tatcgcacgc tccccgtcct gctcgcacag
     4261 ccgatccctc gataccgcct gctcctggcc aaggctgccg cgctcgtgcc gctgatcgtc
     4321 ctgctggcga ttcttgcccg catcgtgttc ctccgagccg ggcgagctgg tatgacgagc
     4381 gcagacgatt ggcttacggc ggcttttgcc ctcgtgccgg taatgggcgt gtgtatcgcg
     4441 ccctggctca cgatggtttt caggagtgtc atcgccggcg tcgtgttcac gatcgccatt
     4501 ccgtccgcgc tctggctctt gatcaacgtc gtacccgctg tgattcgcgg tgtcgacgca
     4561 ccgtacgact actctctcta cctgaccatc ttcggggccg gcaccctggc cgctggtgcc
     4621 attgcgctcg tggacgggcc gcggaggttc ttcaagctcg aggtgacaca agacgcctgg
     4681 gagatcccgc tgagcggagc gcggcgtcac cgggccctgt cagccgcgga aaccggccga
     4741 catcgcaacc ccctggtgca tctcgtcgcc aaggaactca ggctgcaacg tgcggccttc
     4801 ctgctgcctg cgttctatgc actcgcgtgg gcggcgctcg tggccgcgag gtcgaacttt
     4861 tctctgggag cggccttctt cgggatgact ctcatctatt gtggagtgac ggcaatgctc
     4921 gtgggcgctc tggcgagcgc ggaggaacgg gcacttggaa cgctcgccgt tcaagcgctt
     4981 cagccgctcg ccgcctggaa acagtggctg gtcaaggtcg ccattgtgct cgcgctgacc
     5041 gtgacgttca cggtgctgct cccgtcggtc ctggaatggg cgcatccact cagccagggg
     5101 gaggccggct ggaggttttt cggcgcattc ctcgcactca tggccagcag cctttacata
     5161 tcttccctca gcacgggggc gctccaggcc atgctgctct cgatgccgtt tgcggcggtc
     5221 gctgggtggt tgttcgtggt gaccacgtca cagacctcga aattgatctg ggtgccgctg
     5281 tcgcgcgtct ttcgaacgct gagcgtggac tggacgcgac ccatcccgct gctctcgaca
     5341 gccgactccg actggtatat ctggtcgatg agatggctgc aggtggccgt cctgtgtgtt
     5401 ctcgtcgcac tcctgacctg gatggctgcc agaaaccacc gatcggccga acacggcctc
     5461 gggcgcatgc ggcgtcaact cctgtggctc gtgccctgcg tcgtcatggg cggagtgatc
     5521 tccggggccg taccgcccct gctcctctgg ttcctcggta cgcactgacc tcagccccgc
     5581 ggccggcgtt gtagaatcgt cggtcttccc ggaggcccgg ttaatgcccg acatgtccaa
     5641 agcggttcgc gatctctcgg cgtcgttttc cggccgtctg ctgctgtcgg gcgacgccgg
     5701 gtacgaggag gccaggcgcg tgcataacgg cctcatcgac aagcgcccgg ccgtgattgc
     5761 acagtgccgc ggaacggccg acatcagcga cgcggtcgcg ttcgcgcgca cgcacgggct
     5821 gaggatctcg atccgcggag gcggacacaa cgtcgccggc cgcgccgtcg tcgacgcggg
     5881 gatgatgatc gacctctcgc tcatgcgcag cgtcgtcgtg gaccgggcgt cgcgaacggc
     5941 gcgcgtcgaa gggggcgcca cctggaagga ggtcaaccgg gaaacgcagc agttcggcct
     6001 cgccacgacg ggaggcgtgg tcgggacgac gggtgtcgcg ggcctcacgc tcggcggcgg
     6061 cttcggctgg ctcatggcca ggcacggaat gtcgctcgac aacctgcggt cggtggacat
     6121 cgtgctcgca aacggcacgt ccgtccgcgc cagcgctacc gatcacgccg atctcttctg
     6181 ggcggttcgc ggcggcggcg gcaacttcgg cgtcgtctcg tcgttcgagt tcacgctgca
     6241 cgacatcggc cctaccatcg cgggaggact cgtggcctgg ccgatggccg ccgcgcgcga
     6301 cgtgctcagg ttcttcaggg actacacggc ctcgatcggc gatgacgtgt tcacggtgtg
     6361 cgcgctgttg acgggtccgg acggggcgac gaagctcgcc gcgatcgcgg ccgcgtacct
     6421 gggaccggcc accggcgccg aggcggcgct ggcgccggtc aagcgcttcg gatcgccggt
     6481 gatggacgcg atggggccga tttcctacgt cgcgttgaac ggcctgctcg atggcgcctt
     6541 tccgaagggc gcgctcaact actggaaggc gccgttcctg gatcgcatgg aggaccgcgc
     6601 gatcgacgcc gccgtgaccg cgatggcgtc gtgcccctcg ccgatgtcgc agatcctgtt
     6661 cgagcacttc cacg
//
LOCUS       DBNGSH010000140         1751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3575778, whole genome shotgun sequence.
ACCESSION   DBNGSH010000140 DBNGSH010000000
VERSION     DBNGSH010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3575778"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1666)
                     /locus_tag="AC16CY_03330"
     CDS             complement(<1..1666)
                     /locus_tag="AC16CY_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352838.1"
                     /translation="MIEVLSFLVATAAALAAGALVRRLAPAYGAVVPPRPDRWHSVPT
                     PTMGGLAIAFGTVAGFAVLVGGTLEGGILEWSAVLIAALAMCAVGAVDDRLRLSPVAK
                     LVASLAVGAFLVFALAAVEPEGALPTSYTLIGTIWFAGLCHSFNLLDNMDGLASGVAL
                     IAALFMAVLLGPSLGYGLSALLVALAGALTGFLYWNRPRARLFMGDCGSLFIGAVFGG
                     ASLVAVFHQRIAFVSPVVLIVLILVVPLFDTAFVLVLRRLAGRSATRGGTDHVSHRLA
                     SLGFSARTATRILYLLGFIGGLTAWMMLVWSAFEPLLVLLVVALFTVLLVLTGVYLAR
                     VRAYNAEDFQALQKSSFAPFLKDLAFKWHVGEVLLDLVLITVCYYAAYRLRFDRPDEL
                     AAFLPYFTKYLPMVLGVKIAALYTSGLYRRSWETFGFRDLAAVARGVGLGSLLTVVAA
                     YYLDRGEGSSRFVFVLDGLLLALAIFATRASFRTMNLVASAKNKGHRRVLVYGAGGYG
                     RLLVREMRANPAWRMNPVGFIDDDAMKAHRWIGGVPVRGSIEQLERT"
BASE COUNT          360 a          606 c          583 g          202 t
ORIGIN      
        1 tcgtcctctc gagctgttcg atcgaccctc gcacgggcac gcccccgatc cagcgatgcg
       61 ctttcatcgc gtcgtcgtcg atgaagccca ccgggttcat cctccaggct ggattggcgc
      121 gcatctcccg cacgagcagc cggccgtagc cacccgcgcc gtacaccagc acgcgacggt
      181 ggcccttgtt cttcgccgaa gctacgaggt tcatcgtccg gaacgaggcg cgggtcgcga
      241 aaatcgccag cgccagcaac agaccgtcga gcacgaacac gaagcgcgac gacccttcgc
      301 cgcggtccag gtagtatgcc gccaccaccg tcagcagcga cccgaggccg acgcctctgg
      361 cgacggctgc gaggtcgcga aacccgaacg tctcccatga gcgacgatac agccccgagg
      421 tgtacagcgc cgcgatcttg acgccgagca ccatcggaag gtacttggtg aagtacggca
      481 ggaacgcggc gagctcgtcc ggacgatcga atcgaagccg gtacgccgcg taatagcaga
      541 cggtgatgag gacgagatcc agcagtactt ccccgacgtg ccacttgaac gcgaggtcct
      601 tcaggaacgg cgcgaacgag gacttctgga gcgcctggaa gtcctcggcg ttgtaggcgc
      661 ggacgcgcgc gaggtagacg cccgtcagga cgagcagcac ggtgaacagc gcgacaacca
      721 gcagcaccag gagcggctcg aaggcgctcc acaccagcat catccaggcc gtgagcccgc
      781 cgatgaagcc cagaagatag aggatgcggg tggcggttct cgccgagaat ccgagcgagg
      841 ccaggcggtg cgagacgtga tcggtgccgc ctcgcgtcgc gctgcggccc gcgagccgcc
      901 gcagcacgag cacgaacgcc gtgtcgaaca gcggcaccac caggatgagg acgatgagca
      961 cgaccgggct gacgaacgca atgcgctggt ggaacactgc gaccagcgat gcgccgccga
     1021 acacagcccc gatgaagagg ctgccgcagt cgcccatgaa caaccgcgcc ctcggacggt
     1081 tccagtacag gaaccccgtc agcgcgccgg ccagcgcgac gaggagcgcc gacaggccat
     1141 agccaagcga aggcccgagc agcacggcca tgaagagcgc ggcgatgagc gccacgcccg
     1201 aggccaggcc gtccatgttg tcgagcaggt tgaacgagtg gcacaggccg gcaaaccaga
     1261 tcgtgccgat cagcgtgtag ctggtcggca gcgcaccttc cggttccacg gcggcaaggg
     1321 cgaagacgag gaacgccccg acggccaacg aggccacgag cttggccacc ggcgagagcc
     1381 gcaggcgatc gtcgacggct ccgaccgcgc acatcgcgag cgcggcgatg agcaccgccg
     1441 accattccag gatgccgcct tcgagcgtgc cgcccacgag aacggcgaag cccgcgaccg
     1501 tgccgaacgc gatcgcgagc ccgcccatcg tcggcgtcgg gacgctgtgc cagcgatcgg
     1561 gacgaggggg caccaccgcg ccatacgccg gcgcgagacg ccggaccagc gcccctgccg
     1621 ccagcgcggc ggccgtggca acgaggaaac tcagaacctc aatcacgcga tcacgagctc
     1681 acgagctcgg ggctgcgagg caacgtgtcc ctgagcacga acggctcccg ttcgaggatc
     1741 ccgcggcaga t
//
LOCUS       DBNGSH010000141         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1538254, whole genome shotgun sequence.
ACCESSION   DBNGSH010000141 DBNGSH010000000
VERSION     DBNGSH010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1538254"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="AC16CY_03335"
     CDS             complement(<1..223)
                     /locus_tag="AC16CY_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR3352839.1"
                     /translation="MLTKRIIACMDVRDGQVVKGVQFQELRHAGDPAALARRYNVEGI
                     DEVVVLDITATLESRQALARTIDAVAHEIF"
     gene            complement(223..828)
                     /gene="hisH"
                     /locus_tag="AC16CY_03340"
     CDS             complement(223..828)
                     /gene="hisH"
                     /locus_tag="AC16CY_03340"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3352840.1"
                     /translation="MIALVDYGAGNLTSVRKALTALGADFTVPSAPEECRRARGIIVP
                     GVGHFGATAALDAGWRDAIGTSARSGVPLFGICVGMQWLFEGSDEAPGVPGLGLIAGR
                     IARLDGDPLNRLKVPHVGWNSLEIRGGARLLRGLPSGSQVYFTHSYAAPITPECAAST
                     THANTFSSVVERENVFGVQFHPEKSSDAGLTILRNFLEVCG"
     gene            complement(825..1418)
                     /gene="hisB"
                     /locus_tag="AC16CY_03345"
     CDS             complement(825..1418)
                     /gene="hisB"
                     /locus_tag="AC16CY_03345"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR3352841.1"
                     /translation="MKARSATFDRRTTETTIRGRLMIDGRGRYEIRTGIRFFDHMLEL
                     FTRHGGFDLTLDAAGDLDVDQHHTVEDVGIVLGESVLKALGDRKGINRAGYFVMPMDE
                     TLGVAAVDLSGRPHASVDLKMRARMVGDLQSELVHDFFDGFASGARANVHVKVLYGRS
                     SHHHVEAVFKAFARALRVACARDARLAKMLPSTKGLL"
     gene            complement(1415..2455)
                     /locus_tag="AC16CY_03350"
     CDS             complement(1415..2455)
                     /locus_tag="AC16CY_03350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3352842.1"
                     /translation="MRVQGYPDPGAGLRLHLNENTGGCSPRVLEAIQRLKTTDVSTYP
                     SYPQLVRACARHFDVDPEWVLLTNGLDEGILMAAVGHISRARIHDAETIIPLPAFDPY
                     PNSTAAAGATAVGIPPGPDFAFPTDAVIRAITPRTRMIFLNTPSNPTGRLIPIADLRR
                     IAEAAPHAVVLIDEAYIEFGGTTFLPEVARHPNVLVGRTFSKAYGLAGMRVGVVVGQP
                     PALDPVRAVTLPFNINAVAMAATMAALDDREFLPRYAAQVAESRERIYAACRRLGLEY
                     WESAANFVMVRVGSGVADVVKALAERRVHVRDRSHDPATPGCIRVTAGQLEHTDAAVA
                     ALESVISGRSAR"
     gene            complement(2455..>3242)
                     /gene="hisD"
                     /locus_tag="AC16CY_03355"
     CDS             complement(2455..>3242)
                     /gene="hisD"
                     /locus_tag="AC16CY_03355"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR3352843.1"
                     /translation="CPRPEPVVMAAALEAGVSRLFRLGGAHAIAALAYGTDSVPRVDK
                     IVGPGNRWVASAKALVAADCGIDFYAGPTEILVVASRGPSAWIAADLVAQAEHDPDAR
                     AVLITSNRRLAAAVAADVAARVPAGGPARQSLRAHGGIIVTRSMKEAIALANAAAPEH
                     LVVDNETLATQVRSAGSLFVGGWSAQVAGDYAIGSNHVLPTAGAARVRGGLSAADFVR
                     QITVQRVTRSGLSRIGPSVIALARAEGLEAHAASIVARLGGGR"
BASE COUNT          497 a         1151 c         1116 g          478 t
ORIGIN      
        1 ggaagatctc gtgcgccacg gcgtcgatcg tccgggccag ggcctggcgg ctttcgagcg
       61 tggcggtgat gtcgagcacg acgacttcgt cgatcccttc gacgttgtag cgccgggcga
      121 gcgcggctgg atcgcctgcg tgacggagct cctggaactg cacgcccttg acgacctgcc
      181 cgtcgcgcac gtccatgcac gcgatgatgc gcttggtcag catcagccgc acacctcgag
      241 gaagttgcgc aggatcgtga gcccggcatc gctcgacttc tccgggtgga attgcacgcc
      301 gaagacgttc tcgcgctcga cgaccgacga gaaggtgttc gcgtgcgtgg tcgaggccgc
      361 gcactccggc gtgatcggcg cggcatacga gtgcgtgaag tacacctggg atccgctcgg
      421 caaccctcga agcagtcgcg ccccgccgcg gatctcgagc gagttccatc ccacgtgcgg
      481 caccttgagg cggttcaacg gatcgccgtc gagacgcgca attctcccgg cgatgagtcc
      541 cagccccggc actcccggcg cctcgtcgct tccctcgaac agccactgca tcccgacgca
      601 gatgccgaac agcggtactc cggagcgcgc cgacgtgccg atggcgtcac gccacccggc
      661 atcgagggct gccgtggcgc cgaagtgccc gacgcccggc acgatgattc cccgggcgcg
      721 gcggcactcc tccggcgccg acggcacggt gaagtcggcg ccgagcgccg tcagcgcctt
      781 gcgaaccgac gtcaggttgc cggccccgta gtccacgagc gcaatcacaa cagccccttc
      841 gtgctcggca gcatcttcgc cagcctggca tcgcgcgcgc acgcgacgcg cagcgccctc
      901 gcgaacgcct tgaagacggc ttcgacgtga tggtggctcg accggccgta gagcaccttg
      961 acgtgcacgt tcgcccgggc gcccgacgcg aacccgtcga agaagtcgtg caccagctcc
     1021 gactggagat cgcccaccat ccgcgcgcgc atcttgaggt cgacggacgc gtgggggcgg
     1081 ccgctcaggt ccacggccgc gacgcccagc gtctcgtcca tcggcatgac gaagtacccc
     1141 gcgcgattga tgcccttgcg atcgccgagc gccttcagca ccgattcgcc gagcacgatg
     1201 ccgacgtcct ccacggtgtg gtgctggtct acgtcgagat cgccggcggc gtcgagcgtc
     1261 aggtcgaagc cgccgtgcct cgtgaacaac tccagcatgt gatcgaagaa ccggatgccg
     1321 gtccggatct cgtagcggcc gcggccgtcg atcatcagcc tgccgcggat cgtcgtttcg
     1381 gtggtccgcc ggtcgaacgt cgccgagcga gccttcatcg cgcgctcctt ccggagatca
     1441 ccgattccag cgcggcaacg gcggcatcgg tgtgctccaa ctggccggcc gtaacgcgaa
     1501 tgcacccggg cgtcgccgga tcgtgcgatc ggtcgcggac atgcacccgc cgctcggcga
     1561 gcgccttgac cacgtccgcg acgccgctcc cgacgcgcac catgacgaag ttcgcggcgc
     1621 tttcccagta ctcgaggccg agacgacggc aggcagcata gatgcgctct ctcgattcgg
     1681 cgacctgcgc cgcgtagcga gggaggaatt cccgatcgtc gagcgcggcc atggtggcgg
     1741 ccatggcgac ggcgttgatg ttgaacggca aggtgactgc ccgcacgggg tcgagcgccg
     1801 gcggctggcc gacgaccacg ccgacccgca tgccggccag gccgtacgcc ttcgagaacg
     1861 tccggccgac caggacgttc gggtgcctgg ccacctcggg caggaacgtc gtgccgccga
     1921 actcgatgta cgcctcgtcg atgagcacga cggcatgcgg cgccgcctcg gcgatcctcc
     1981 gcaggtccgc aatcgggatg aggcggcccg tcggattgct cggcgtgttg aggaagatca
     2041 tccgagtccg cggcgtgatg gcgcggatga cggcatcggt cgggaacgcg aaatccggcc
     2101 ccggcgggat gccgaccgcc gtcgcgcccg cagccgccgt cgagttcggg tacggatcga
     2161 atgcaggcag cgggatgatc gtctcggcgt cgtgaatccg cgcgcgcgag atatgtccga
     2221 cggcggccat caaaatgccc tcgtccaggc cgttggtgag cagcacccac tccgggtcca
     2281 cgtcgaagtg cctcgcgcac gcccgcacga gctgcggata cgacggatac gtggacacgt
     2341 ccgtcgtctt caggcgctgg atggcttcga gcacgcgcgg cgagcatccg ccggtgttct
     2401 cgttgaggtg cagccgcagc cccgcgccgg gatccgggta gccttgcacc cgcattacct
     2461 gccacccccg aggcgcgcga cgatcgaggc ggcgtgcgcc tcgagccctt cggcccgcgc
     2521 cagcgcgatc acgctgggac cgatccggga cagccccgac cgggtcaccc gctgcacggt
     2581 aatctgcctc acgaagtcgg cggcagacag gccgccgcgc acgcgcgcgg cgccggcggt
     2641 cgggagcacg tggttcgagc cgatcgcgta gtcgccggcc acctgggccg accagccgcc
     2701 cacgaagagc gaccccgcgc tcctcacctg cgttgccagc gtctcgttgt ccacgacgag
     2761 atgctcggga gcggcggcgt tggcaagcgc gatcgcttcc ttcatcgatc gcgtgacgat
     2821 gatgccgccg tgagcgcgga gtgattgacg ggccgggccg ccggccggca ctcgcgcggc
     2881 gacgtccgct gcgaccgcag cggcaagccg ccggttcgac gtgatgagta ccgcgcgcgc
     2941 gtcagggtcg tgttccgcct gcgccacgag gtccgcggcg atccatgcgg agggcccccg
     3001 cgaggcgacg accaggatct cggtcggccc cgcgtagaag tcgatgccgc agtcggctgc
     3061 gacgagcgcc ttggccgagg cgacccagcg gttgcccggt cccacgatct tgtccacgcg
     3121 gggaaccgag tccgtgccgt atgcgagagc ggcgatcgcg tgcgcgccgc cgagtctgaa
     3181 caggcgcgag acgcccgctt cgagcgcggc ggccatcacg accggttcgg ggcgcgggca
     3241 ga
//
LOCUS       DBNGSH010000142         1988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_179961, whole genome shotgun sequence.
ACCESSION   DBNGSH010000142 DBNGSH010000000
VERSION     DBNGSH010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_179961"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="AC16CY_03360"
     CDS             complement(<1..1581)
                     /locus_tag="AC16CY_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3352844.1"
                     /translation="MAQRRRITVDGNEAAASVAYRASETIAMYPITPSSPMAEVCDEW
                     STRKRANLWGAIPELAEMQSEGGAAGAVHGALQAGALATTFTASQGLLLMIPNMYKIA
                     GELSPFTMHVAARTLATHALSIFGDHSDVMACRQTGFALLCSNSVQEAHDMAAVAHAA
                     TLESRIPFLHFFDGFRTSHEVAKLEELSDDDLRTLIPESTIFAHRKRALTPDRPVLRG
                     TAQNPDVFFQAREAANGFYDACPAIVERTMKALSALTGRHYGLFEYCGDRGAERVIVL
                     MGSGAETAQETTEYLAARGEKVGLLKVRLYRPFSRSAFLAALPPSARSLAVLDRTKEP
                     GAPGDPLYLDVTTALAEAQAEGISPFAAPPRIIAGRYGLSSKEFTPAMVKAVFDELAK
                     AAPKRHFTVGIVDDVTQTSLRWDPSFRTEADEVSASVFYGLGADGTVGANKNSIKIVG
                     QETDLFAQGYFVYDSKKSGSQTISHLRFGPRPISAPYLVAQASFVGCHQFGLLERQEV
                     LDRAAPGAALLLNSRHAHN"
     gene            1692..>1988
                     /locus_tag="AC16CY_03365"
     CDS             1692..>1988
                     /locus_tag="AC16CY_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352845.1"
                     /translation="MNGPTAVAIGTALVCMGLAPLSAAERRSIGLTARDAPIDALVVP
                     GATESAPTVLLVAGLQGPDASVDAVRQEAAAFEALAPARRSFRLIAIPLSNPDAV"
BASE COUNT          310 a          670 c          684 g          324 t
ORIGIN      
        1 gttgtgtgcg tgccgggagt tgagcagcag cgccgctccc ggcgccgccc ggtcgagcac
       61 ttcttgacgc tcgagcagcc cgaactggtg gcagccgacg aagctggcct gggcgacgag
      121 gtagggcgcg ctgatcggac gcggcccgaa gcgcaggtgc gagatggtct gcgacccgga
      181 cttcttggag tcgtagacga agtagccctg cgcgaagaga tccgtctcct ggccgacgat
      241 cttgatcgag ttcttgttgg cgccgaccgt gccgtcggcg ccgaggccgt agaacaccga
      301 cgccgacacc tcgtccgcct cggtccggaa ggacggatcc cagcggagcg aggtctgcgt
      361 cacgtcgtcg acgatgccga cggtgaagtg ccgcttcggc gccgctttgg cgagctcgtc
      421 gaagaccgcc ttcaccatcg cgggcgtgaa ctctttcgac gacagtccgt agcggcccgc
      481 gatgatcctc ggtggagccg cgaaggggga gatcccttcg gcctgcgcct cggcaagcgc
      541 ggtcgtcacg tcgaggtaaa gcgggtcgcc gggggcgccg ggctccttcg tccgatcgag
      601 cacggcgaga ctccgggcgc tcggaggcag cgccgcaagg aacgcgctgc gcgagaacgg
      661 gcgatagaga cggaccttca gcaggccgac cttctcgccg cgggcggcca ggtattccgt
      721 ggtttcctgc gcggtctcgg cgcccgagcc catcaggacg atgacgcgct cggcgccccg
      781 atcgccgcag tactcgaaca atccatagtg ccgcccggtc agcgcagaaa gcgccttcat
      841 cgtccgctcg acgatcgccg ggcacgcgtc gtaaaacccg ttggcggcct cgcgcgcctg
      901 aaagaacacg tccgggttct gcgccgtgcc gcggagcacg gggcgatcgg gcgtcagagc
      961 acgtttgcgg tgagcgaaga tcgtcgattc cggaatcagc gtccgcaggt cgtcgtcgct
     1021 gagctcctcg agcttcgcca cctcgtgcga agtccggaac ccgtcgaaga aatgcaggaa
     1081 ggggatgcgc gactcgagcg tcgcggcgtg tgccacggcg gccatgtcgt gcgcctcctg
     1141 cacggagttc gagcacagga gggcgaagcc cgtctggcgg cacgccatga catcggaatg
     1201 gtccccgaag atcgacaacg catgcgtcgc gagcgtgcgc gcggctacgt gcatcgtgaa
     1261 gggcgacagc tcgcccgcga tcttgtacat gttcgggatc atcaggagca ggccctgcga
     1321 cgcggtgaag gtggtcgcga gagcgccggc ctggagcgcg ccgtggacgg cgccggccgc
     1381 gcctccttcg gactgcattt cggccagctc ggggatggcg ccccacaggt tggcccgctt
     1441 gcgagtggac cactcgtcgc acacttccgc catggggctg ctcggcgtga tcgggtacat
     1501 cgcaatcgtc tcgctcgcgc gatacgcgac cgacgccgct gcctcgtttc cgtctaccgt
     1561 gatccgccgc ctctgtgcca tgacaggccc ccgtccaatg acaaagtccg gcacgtgaat
     1621 cgccggtggt cgctttcctt cgcgggtgag aaaagaagcc catggtatct gatgtctcat
     1681 actatgcatg cgtgaacgga ccgactgccg ttgccatcgg aacagcactc gtctgcatgg
     1741 gccttgcgcc gctgtcggca gccgagcgcc ggagcatcgg gctcacggcc cgcgacgccc
     1801 cgatcgacgc gctcgtggtg cctggagcca cggagtcggc gcccacggtg cttctcgtcg
     1861 ccggtctcca gggacccgac gcgagcgtcg atgccgtccg gcaggaagcc gcggcgttcg
     1921 aggcgctggc gccggcccgc cgctccttcc gcctcatcgc gattcccctg tcgaatcccg
     1981 acgccgtg
//
LOCUS       DBNGSH010000143         7076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3066921, whole genome shotgun sequence.
ACCESSION   DBNGSH010000143 DBNGSH010000000
VERSION     DBNGSH010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3066921"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="AC16CY_03370"
     CDS             complement(<1..926)
                     /locus_tag="AC16CY_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR3352846.1"
                     /translation="MTSSPQLLLSIFVSDLLPVFVIAGIGFLLARFAGVQVHTISRLT
                     FHALAPALVFDILVTSTLESGQFGRMALFYAAVASSAALMAAVAAIPLRLDRRALSAL
                     LLVVICSNSGNYGLPVTMLAFGREALAYASVYFVTSATFTYTGGILLAASGRRAVRDA
                     IAGLVRIPAVYGAAAAAAVIGLGLPMPSAVQTPITLLGNAAVPMMILVLGMQLERAAM
                     PKRPAVVAAAVLISLVLTPLAALGIGRLVGLQGAAFQAGVLQASMPTAVITTILALEF
                     DALPDFVTSVVFVSTLLSPFTVTLMIAYLKGL"
     gene            1007..2380
                     /locus_tag="AC16CY_03375"
     CDS             1007..2380
                     /locus_tag="AC16CY_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3352847.1"
                     /translation="MKTVDVGAVIDEGRWTGYQKLLIFGTALTIILDGVDNQLLPNAV
                     PALIKEWGRPRADFINALALGPFGMMIGGLLGGILGDRIGRRSALLASVLTFAVVTLA
                     IAFVDTIAMLGALRFLAGVGLGGAMPNAATLASEYVPARRRPFAVTLTIVCIPLGGAL
                     AGELAARVIPGYGWRTLFIFGGIIPIVLALVLWKVLPESPRYMARRRNRWAELTRTMR
                     RIGHDIPDDVEYTEAAASGAPVTTASIGSLFAPGLARDTLALCASFFFCLMVNYVVIL
                     LLPAMLTADEVGFAQPAANRALAISNYGGVAGAILGALVIQRLGSRVAMLGMSAGAIV
                     CAVVLTGTTLDPQDTLFLMTMILLSGALLNGVQTTMYALAAHVFPTNIRSTGVGTAVA
                     VGRVGNVLAAYVGEYSLRGGGPPGYFTSIAVLMGVVLLSLAMVRRHVPRTSGVPALAA
                     AGVPGRR"
     gene            2477..3877
                     /gene="ligM"
                     /locus_tag="AC16CY_03380"
     CDS             2477..3877
                     /gene="ligM"
                     /locus_tag="AC16CY_03380"
                     /EC_number="2.1.1.341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046274 - lignin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanillate/3-O-methylgallate O-demethylase"
                     /protein_id="HIR3352848.1"
                     /translation="MSQKSLEQVLQAAKSPVDLLRNSKIGAYVYPVVPSEYTNWRDEQ
                     RAWRDSAVLFDQSHHMAELLVTGPDALKMLTYLGTNSFAGYAPNRAKQFAPCSYDGYV
                     IGDVIMFYLAENEFNLVGRAPTINWVEFHAKSGAFKVDVQRDDRSPSNPKGQAIVRRH
                     YRYQIQGPNAAKVLERLNGGPVPDVKFFHMDTITIKGRKVRALRHGMAGAPGLEVWGP
                     YEQREEIREAIIEAGKDFGLVQVGARAYATNTLESGWIPSPLPAVYTGEKMKAYREWL
                     PAAGYEATGSIGGSFVSNNIEDYYVTPYELGYGSFVKFDHDFIGREALEKKSKEKNRK
                     KVTFVWNGEDVARVIAPMFTPGAERYKYIDLPLSNYASSSYDKVMKGGKMVGLSMFGG
                     YSYNEQSMLSLGVVDPDVNIGDALTLVWGEEGGGTGKTTVERHKQTEIRVTVGPTPYN
                     ETARTTYRPTSTVGTR"
     gene            complement(3844..4740)
                     /locus_tag="AC16CY_03385"
     CDS             complement(3844..4740)
                     /locus_tag="AC16CY_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3352849.1"
                     /translation="MADRYTDVTKVLYRVLVLNLAVALAKILLGYGTGAVSIVTDGFH
                     SLTDSASNVVALVAISVARQPPDANHPYGHRKYETIASIAILVFLVVVLVEVLSAAVD
                     RFLNGGTPRVFPEGIGLMAATLVVNVLVVSYELREGRRLNSEVLRADARHTRSDVLTS
                     GAVLGALFGVWFGYPLLDPIAALLVAGFIGHACWSIAREASRILSDQIVIPEEQVRAV
                     VQSVPAVIGCEKIRTRGAADHAFLDLHLWLEGSTPLRDAHATSHVVKDRLMARFPQLA
                     DVVIHIEPPPDTYRVPTVDVGR"
     gene            complement(4756..5307)
                     /gene="lepB"
                     /locus_tag="AC16CY_03390"
     CDS             complement(4756..5307)
                     /gene="lepB"
                     /locus_tag="AC16CY_03390"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3352850.1"
                     /translation="MDPPADALSQRLHIWKEELVAWFKTLFSAAVYATLIVAFGFQVA
                     RVEGQSMAPTLSDQDRLIVNKFAYRWGAPRRGDIVMLYYPINPDKSFVKRVIAEENDE
                     VRMVDGRVYVNDVPVADEFVPPEYRGHDDFGPRRIDEGYYFVMGDHRNNSSDSRHWGL
                     VPKKYIIGRVQLRWWPIPNARVF"
     gene            complement(5364..6158)
                     /locus_tag="AC16CY_03395"
     CDS             complement(5364..6158)
                     /locus_tag="AC16CY_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3352851.1"
                     /translation="MSKIRVLVVDDEQLARDELCYQLGQVGDVEVVGQAADGPEALDA
                     IDKHEPDLVFLDIQMPGLGGFEVARRLFERGEDAPALVFVTAFDQHAIEAFEVNAVDY
                     LLKPVETGRLEQALLRARRRVEADRQTPAGTRPANQQLEQIVKMMSGRQIRREQVVIR
                     VGERFMLVQADEIIYASLVDDSINIVTGQVAGTSNYRTLDDLQARLDSDVFWRVHRSH
                     LVNIYKIKEIVPWFSRNYILRMKDAKGTEIPVSRTQTKRLREYLKL"
     gene            complement(6155..>7076)
                     /locus_tag="AC16CY_03400"
     CDS             complement(6155..>7076)
                     /locus_tag="AC16CY_03400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3352852.1"
                     /translation="KEAIWQFTPFVFVDLRKHVWQMIRKFEPNWQLILLLATVALELL
                     RQTLGTRFGEHRLFFLGSPSSPWMTLLVVLVTVVAVATPIKIWNSARIEHRLQEQAQL
                     LLAAKIEALKSQINPHFLFNTLTSISSLIRSQPDTARTLILKLSALLRRLLRSHQHFV
                     TLREELESIDEYLDIEVIRFGPKLRVRKEISPDTLDVVVPSMILQPLVENSIKHGFAR
                     KVGPGAITIRSHRENGRAVIEVEDDGMGFVLDSLDQPMTSGIGLSNVRERLAVIYGDQ
                     YQLKLRSEPGRGTSARIEVPELTAAQQITA"
BASE COUNT         1236 a         2284 c         2353 g         1203 t
ORIGIN      
        1 tacaggccct tcagatacgc gatcatcagt gtgacggtga aggggctgag cagcgtcgag
       61 acgaagacga cgctggtcac gaagtcgggc agcgcgtcga actcgagggc gaggatcgtc
      121 gtgatcacgg ccgtcggcat cgatgcctgc agcactccag cctggaatgc cgcgccttgc
      181 aggccgacga gtcggccgat tccgagcgcc gcgaggggcg tgaggacaag agagatgagc
      241 acggccgcgg cgacaaccgc cggccgcttt ggcatcgccg cccgctccag ctgcatgccc
      301 agcacgagaa tcatcatcgg caccgccgca ttgccgagca gcgtgatcgg cgtctggacg
      361 gccgacggca tggggagccc caggccgatc accgccgccg cggccgccgc tccatagacg
      421 gccggaatcc tgacgagacc ggcgatcgcg tcgcgcacag ctcgccggcc gctcgcggcc
      481 agaaggattc cccccgtata ggtgaacgtc gcgctcgtca cgaaatacac gctggcgtac
      541 gcgagcgcct cgcggccgaa cgcgagcatc gtcaccggga gcccgtagtt gccgctgttc
      601 gagcagatca ccaccagcag cagggcgctg agggcgcggc gatcgaggcg cagcgggatg
      661 gccgcgacgg ccgccatgag cgcggccgac gaggccacgg ccgcgtagaa gagcgccatc
      721 cggccgaact gtccgctctc gagggtcgag gtgacgagga tgtcgaacac gagcgcgggg
      781 gcgagcgcgt ggaacgtcag gcgcgagatc gtgtgcacct ggaccccggc gaatcgagcc
      841 agcagaaagc cgatgccggc gatgacgaac accggcagga gatcggacac gaagatcgag
      901 aggaggagct gcggcgaact cgtcacgtca acacggatca tatgcggttc gagaggcctc
      961 gccgcaccgc gtcggcgaag gcgggcgctg gtatacttcc gcacccatga agaccgttga
     1021 cgtcggcgcg gtgatcgacg agggccgctg gaccggctac cagaagctgc tcatcttcgg
     1081 cacggccctc acgatcattc tcgacggcgt tgacaatcag cttctcccga acgccgttcc
     1141 agcgctgatt aaagagtggg ggcggccccg cgccgacttc atcaatgcgc tggcgctcgg
     1201 tccctttggc atgatgatcg gcggtctgct cggcgggatc ctgggcgatc gcatcggtcg
     1261 gcgttcggcg ctgctggcca gcgtcctcac gttcgccgtc gtgacgctcg cgattgcctt
     1321 cgttgacacg atcgcgatgc tcggcgccct gcggttcctg gccggcgtgg gcctgggtgg
     1381 cgccatgccc aatgcggcga cgcttgcgtc cgagtacgtg ccagccaggc ggcgtccttt
     1441 cgcggtgacg ctcaccatcg tctgcattcc gctcggcggc gcccttgccg gcgagctcgc
     1501 cgcccgcgtg attccgggct acggctggcg gacgctcttc atcttcggcg gcatcatccc
     1561 gatcgtcctc gcgctcgttc tgtggaaagt cctcccggag tcgccccgct acatggcgcg
     1621 gcgtcgcaac cggtgggccg agctcaccag gacgatgcgt cgcatcgggc acgacatccc
     1681 ggacgacgtc gagtacacgg aagccgcggc ctccggtgct ccggtcacaa ccgcatcgat
     1741 cggcagcctg ttcgcgccgg gactcgctag ggacacgctg gcgctctgcg cttccttctt
     1801 cttctgcctg atggtcaact atgtcgtcat cctgctgctg cccgcgatgc tcaccgccga
     1861 cgaggtcggg ttcgcgcagc cagcggcgaa ccgcgcgctc gcgatatcga actacggcgg
     1921 cgtcgcaggc gcgatcctcg gcgcgctcgt catccagcgt ctgggttcgc gcgtggccat
     1981 gttggggatg tctgccgggg cgatcgtctg cgcggtggtg ttgacgggga caacgctcga
     2041 tccgcaggac acgctgttcc tgatgacgat gatcctgctg agcggcgccc ttctcaatgg
     2101 cgtgcagacg acgatgtacg cgctggccgc gcacgtgttt cccacgaaca tccggagcac
     2161 gggcgtcggg acggcagtgg ccgtgggccg cgtcgggaat gtgcttgcgg cctatgtcgg
     2221 ggagtactcg ctccgcggag gcggcccacc cgggtatttc acgtcgatcg cagtgctgat
     2281 gggcgtcgtg ctgctctccc tggccatggt gcggcgccac gtgcctcgaa cgtccggcgt
     2341 tccggccctt gcggcggcgg gcgtgcccgg acgtcgctga gacgtttgta gtctgcccct
     2401 ccggccggta ataatctaag gcttgcctgg gcggaggccc gccatacaac ggcatacaca
     2461 ggagttacga gaggtcatga gtcagaaaag cttggagcag gtattacagg ccgccaaaag
     2521 cccggttgat ttgctgcgga actcgaagat tggagcctac gtgtaccccg tggttccatc
     2581 cgagtacacc aactggcgcg acgaacagcg cgcatggcgc gactcggcgg tcctcttcga
     2641 ccagtcccat cacatggcgg agctgctcgt cacgggtccg gacgccctca agatgctgac
     2701 ctacctcggc accaacagct tcgcgggcta cgccccgaac agggccaagc agttcgcgcc
     2761 gtgcagctac gacggctacg tgatcggcga cgtgatcatg ttctatctcg ccgagaacga
     2821 gttcaacctg gtcgggcgcg cgccgacgat caactgggtg gaattccacg cgaagtccgg
     2881 ggcgttcaag gtggacgtgc agcgcgacga ccgctcgccc tcgaacccca aaggccaggc
     2941 gatcgtccgc cggcattacc ggtatcagat ccaggggccg aacgcggcga aagtcctcga
     3001 acggctgaat ggcgggcccg ttccggacgt caagttcttc cacatggaca ccatcacgat
     3061 caagggacgc aaggttcgcg cgctgcgtca cgggatggcc ggcgcccccg gcctggaggt
     3121 gtggggcccc tacgaacagc gcgaggagat ccgcgaggcg atcatcgagg ctggcaagga
     3181 cttcggcctg gtccaggtgg gcgcccgcgc gtatgcgacc aacacgctcg aatcaggatg
     3241 gattccctcg ccgctgccgg ccgtgtacac cggcgagaag atgaaggcct atcgcgagtg
     3301 gctgcccgcc gccgggtacg aggccacggg ctccattggt ggaagcttcg tgtcgaacaa
     3361 catcgaagac tattacgtga cgccgtacga gctgggctac gggtcgttcg tgaagttcga
     3421 ccatgatttc atcggccgcg aggcgctcga gaagaagtcc aaggagaaga accgcaagaa
     3481 ggtcaccttc gtgtggaacg gcgaggatgt cgcccgggtc atcgcgccga tgttcacgcc
     3541 tggggccgaa cgctacaagt acatcgatct gccgctgtcg aactacgcct cgtcctccta
     3601 cgataaggtg atgaaggggg gcaagatggt cggcctgtcg atgttcggcg gctacagcta
     3661 caacgagcag tcgatgcttt cgctcggcgt cgtcgatccg gacgtcaaca tcggcgacgc
     3721 gctcacgctc gtctggggcg aagagggcgg cggcacgggg aagaccacgg tcgagcgcca
     3781 caagcagacc gaaatccgcg tcaccgtcgg tcccacgccg tacaacgaga cggcccggac
     3841 gacctaccgg ccgacgtcga cggtgggcac gcggtaggta tccggcggcg gctcgatgtg
     3901 gatcaccacg tcggccagct gcgggaacct cgccatcaac cggtccttca cgacgtgcga
     3961 cgtggcgtga gcgtcgcgaa gcggggtgct gccttcgagc cacaggtgga ggtcgaggaa
     4021 cgcgtggtcc gcggcacctc gcgtcctgat cttctcgcag ccgattacgg ctggcaccga
     4081 ttggacgacc gcgcggacct gctcctctgg gatgacgatc tggtccgaca ggatgcgcga
     4141 ggcctcccgc gcgatgctcc agcacgcgtg cccgatgaac cctgccacga gcagcgccgc
     4201 aatcggatcc agcaggggat agccgaacca cacgccgaac agcgcgccga gcaccgcgcc
     4261 tgacgtgagg acgtcactcc tggtgtggcg ggcgtcggcg cgcagcacct cgctgttgag
     4321 acgccgccct tcccgcagct cgtacgagac gacgaggaca ttgacgacga gcgtcgccgc
     4381 catgaggccg atgccctcgg gaaacacccg cggcgtgccg ccgttcagga agcgatccac
     4441 cgccgcgctc agtacctcta cgagcacgac gaccaggaag accaggatcg cgatcgaggc
     4501 gatcgtctcg tacttacggt ggccgtacgg gtggttggcg tcgggcggct gacgcgcgac
     4561 cgagatggcg acgagcgcga cgacgttgga cgccgaatcg gtcagggagt gaaacccgtc
     4621 ggtgacgatc gagacggcgc cggtgccata gcccaggagg atcttggcca gggcgacggc
     4681 gaggttcagg acgagtactc gatacaggac cttcgtgaca tcggtgtatc ggtcagccac
     4741 gcttgcgcgc gagcgctaga acacgcgggc gttcgggatc ggccaccagc gaagctgcac
     4801 gcggccgatg atgtatttct tcgggaccag gccccagtgg cggctgtcgg agctgttgtt
     4861 ccggtgatcg cccatcacga agtagtagcc ctcgtcaatc cgtcgggggc cgaagtcgtc
     4921 gtggccgcgg tattcagggg gaacgaactc gtccgccact ggaacgtcgt tgacgtacac
     4981 ccggccatcg accatgcgca cttcatcgtt ctcttcagcg atgacccgtt tgacgaacga
     5041 cttgtcggga ttgatcgggt aatacagcat cacgatgtcg ccgcggcgag gcgcacccca
     5101 gcggtaggcg aacttgttca cgatcagtcg atcctgatcg gaaagcgtcg gtgccatgct
     5161 ctggccttcc acgcgcgcca cctgaaagcc gaacgcgacg atgagcgtgg cgtagacggc
     5221 ggcggagaac agcgtcttga accaggcgac gagctcttcc ttccagatat gcagccgctg
     5281 cgacagggcg tcggccgggg ggtccatgac ctccaggcgc agaggtgccg tccctccgct
     5341 ggcatccggc tccaggggtg cgcctagagt ttcaggtact ctctgagccg cttggtctgc
     5401 gtgcggctca ccggtatttc cgtgcccttc gcgtccttca tccggaggat atagttcctg
     5461 ctgaaccaag ggacgatctc cttgatcttg tagatgttca ccaggtgcga cctgtggacg
     5521 cgccagaaga cgtcggagtc cagccgcgcc tgcaggtcgt ccagggtccg gtagttggac
     5581 gtaccggcca cctgccctgt aactatgtta atcgagtcgt cgaccagcga ggcatagatg
     5641 atttcgtcgg cctgaacgag catgaaccgt tccccgaccc ggatcacgac ctgttcccgg
     5701 cgtatctgcc tgccggacat cattttgacg atctgttcga gctgctggtt cgctggccgg
     5761 gtgccggccg gcgtctgccg atcggcctcg acccgccgcc gggcgcgcag cagggcctgt
     5821 tcgagccgcc cggtttcgac cggcttcagc aggtagtcca cggcgttgac ttcgaacgcc
     5881 tcgatggcgt gctgatcgaa ggcggtcacg aagacgagcg cgggggcatc ctccccccgt
     5941 tcgaacagcc ggcgggccac ctcgaaccct cccaggccgg gcatctggat atcgaggaac
     6001 accaggtcgg gctcgtgttt gtcgatggcg tccagcgctt cagggccgtc ggcagcctgg
     6061 ccgaccactt cgacgtcccc gacctgaccg agctggtaac acagctcgtc ccgggcgagc
     6121 tgttcgtcgt ccacgaccag gacccgtatc ttgctcatgc tgtgatctgc tgggccgccg
     6181 tcagttccgg gacctcgatg cgcgcgctcg ttccgcggcc cggttcactc ctgagcttca
     6241 attggtactg gtcgccatag atcacagcca gccgctcgcg tacgttggac aaaccgatgc
     6301 cgctcgtcat cggctgatcc aggctgtcga ggacgaatcc catcccgtcg tcctccacct
     6361 cgatgacggc ccgcccgttc tcacggtggc tgcggatcgt gatggctccc ggtccgacct
     6421 ttcgcgcgaa gccgtgcttg atcgagttct cgacgagcgg ctgcaggatc atgctgggga
     6481 cgacgacgtc gagcgtatcc gggctgatct ccttccgaac gcgcagcttc ggcccgaacc
     6541 ggatgacctc gatgtcgagg tactcgtcga tcgactcgag ctcctcgcgc aaggtgacga
     6601 agtgctgatg gctgcggagc agccgccgca gcagcgccga cagcttgagg atgagcgtgc
     6661 gcgccgtgtc gggctgcgag cgaatcaacg aggaaatcga cgtcagcgtg ttgaacagga
     6721 agtgcggatt gatctggctc ttgagcgcct cgatcttcgc ggcgagcagg agctgcgcct
     6781 gctcctgcag gcggtgctcg atgcgtgccg agttccagat cttgatcggc gtggcgacgg
     6841 ccacgaccgt cacgagcacg acgaggagcg tcatccatgg cgaggaggga gacccgagaa
     6901 agaagaggcg atgctcgccg aagcgcgtgc ccagcgtctg gcgcagcaac tcgagcgcga
     6961 cggtcgccag cagcaggatc aactgccagt tgggctcgaa cttccggatc atctgccaga
     7021 cgtgcttccg cagatccacg aacacgaacg gcgtgaattg ccagatcgcc tccttg
//
LOCUS       DBNGSH010000144         4634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2387277, whole genome shotgun sequence.
ACCESSION   DBNGSH010000144 DBNGSH010000000
VERSION     DBNGSH010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2387277"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2456)
                     /locus_tag="AC16CY_03405"
     CDS             complement(<1..2456)
                     /locus_tag="AC16CY_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352853.1"
                     /translation="MEIPARLRALFEALFTDLRDASRSLRRSPGFACAAVVTLAVSIG
                     ANTAIFTVVEAVVLAPFGFSEPDRLVAIRASAPGSDVRGEIGVGAEFYVQYRENAKTL
                     EDLGMLQTAQTTVHAGTQSERLFIAAASPSVFSTLRVKPIIGRLPTDADEEAEVAVIS
                     HWLWTTWFGGDASILGKPIEVSGRLRTVVGVMGPEFGFPNERMSLWMHDQVTGPITPG
                     NFDLVLVGRMAPGANHQTVAAELATLARRLPERFGGPPAYRQFIERHVPVVRALDEVL
                     IGSIARPLWILLGTVGIVLLVACANVANLLLVRAESRKRDVAVRRALGAGRGGLIRWQ
                     MAEAAVLAVFGAAGGVLLAWIGLPLLVRAAPENIPRLSSVALDSTALLFTLGVAVAAA
                     CVSGLLPAIRFSGSNILDDLRQSTRVASRSSHLTRQALVVVQTAAALVLLVASGLLTQ
                     SVRALNRVDPGFDIENIFTFQVAPDFAKRGLTDAPSFAQFHYALMDRLAAMPGVESVG
                     VVDRLPLDEGAAAGRIATRRTEASGAIEPLVRLTFTGGDYFQTMGIRLLKGSYFPRSA
                     NPTNDVRVIVSARAANLLWPGEDPIGQVLRRAGSGTPWMTVQGVVEDVMLANFREEAP
                     QPLVYMPMVGQTARSWIVGTPAYVVRTARAETIAPEIREVIRQIMPEAPMYRVFTMAG
                     LAARSMSALSFTMLMLAIASGLALILGAVGLYGILSYVVSQRRKEIGIRMALGAQAAA
                     VRRLVVAQGVRVAMVGVAVGLIGAFALTRVLASLLFGVAAIDLATFAAMSGVMAGVAL
                     LASYGPARRASGANPIAALRA"
     gene            2617..4434
                     /locus_tag="AC16CY_03410"
     CDS             2617..4434
                     /locus_tag="AC16CY_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3352854.1"
                     /translation="MFVRVGVSLLLFVGLSAPAFAQTPDGEAVFKQACASCHRDGQTQ
                     APTRETLRQMTPEAILNSLTLGRMMVQAISLSEPEQRAVSVALAGRPFAPPSPPVVVN
                     RCATSPPMRDPAGPGGWSGWGNGVSNTRFQSAANGGLTAADLPKLKLKWAFGYSIVTS
                     ARAQPTLAGGRLFVASENGEVHALDPKTGCTHWTFKAQAGVRTAPSAGPYKSAAGAAG
                     YAVYFGDARTNVYAVDANTGQQIWMRKVDEHRSASLTGAPTVYGGKVFVPVQGLNEEG
                     QGGNAKYECCTFRGSVSALDASTGSVLWKTYTIDEPKPRAKNKNGIQQWGPAGAGIWS
                     APTVDPKRNMVYVATGNNYADPPARTSDAIVAMDMNSGTIKWVSQTTPNDNWTMGCRP
                     ENPDNPNCPAKMGPDHDFSASPSLATVNGRDLLVLPQKSGMAYALDPDAEGQKVWEFR
                     IGQGSGLGGQWGGAVDGQNAYFGVSDLMSPNPGGIRAVNLATGQLAWSVGPQTRLCDA
                     KRPTCRASQGAAVTAIPGAVLSGSLDGGMRAYSATDGSIIWTFDTNKEFETVNGVKAN
                     GGGLEGPGPIVAGGMLFFNSGYGGFVGNPGNVLLAFGID"
BASE COUNT          831 a         1569 c         1595 g          639 t
ORIGIN      
        1 gcccgcagcg ccgcgattgg attcgcccct gacgcccgtc gcgcgggacc gtagcttgcg
       61 agcagcgcga caccggccat cacgcccgac atcgccgcga acgtggccag gtcgatggct
      121 gccacgccga acaggaggct cgcaagcacg cgcgtcagcg cgaacgcccc aatcaggccg
      181 accgctacac cgaccatcgc cacgcgcaca ccttgcgcaa cgaccaggcg acggacggcg
      241 gccgcctggg cccccagcgc catcctgatc ccgatctcct tcctccgctg cgagaccacg
      301 tacgacagaa tgccgtagag cccgacggcg ccgaggatca aggcgagccc agaggcgatc
      361 gcgagcatca gcatggtgaa cgagagcgcc gacatcgacc gtgccgcaag gcccgccatc
      421 gtgaagacgc gatacatggg cgcctccggc atgatctggc ggatcacctc gcgaatttca
      481 ggagcgattg tctcggctcg ggcggtccgc acgacgtacg cgggtgtgcc gacgatccag
      541 ctgcgcgccg tctggccgac catgggcata tacacgagcg gctggggtgc ctcctccctg
      601 aagttggcca gcatcacgtc ttcgacgaca ccctgcaccg tcatccacgg cgtaccggat
      661 ccggcgcggc gcagcacctg gccgatcggg tcctcccccg gccacagcag gttggcggcc
      721 cgcgcgctga cgatgacgcg gacgtcgttt gtcggattcg cgcttcgcgg gaagtagctc
      781 cccttcagca ggcggatccc catcgtctgg aaatagtccc cgccggtgaa cgtgaggcgg
      841 acgagcggct cgatcgcgcc gctcgcctcg gtgcgtcgcg tggcgatgcg tccggcagcc
      901 gctccttcgt ccaggggaag cctgtctacg acgcccaccg actcgacgcc gggcatcgcg
      961 gccaggcggt ccatgagcgc gtagtgaaac tgcgcgaacg acggcgcatc ggtgaggccg
     1021 cgcttcgcaa agtcgggcgc cacctggaag gtgaagatgt tctctatgtc gaatcccgga
     1081 tcgacgcggt tgagggcacg gacgctctgc gtgaggaggc cggacgcgac gagcagcacg
     1141 agagcggcag ccgtctgcac gacgacgagc gcctgacgcg tgagatgact cgaacgagag
     1201 gccactcgcg tcgattgcct gagatcgtcc aggatgttcg agccggagaa gcgaatcgcc
     1261 ggaagcaggc ccgacacaca cgcggcggcg accgcgacgc ccagggtgaa gagaagcgcg
     1321 gtcgagtcga gggcaacgga actcaatctc gggatgttct cgggagcggc gcgcacgagc
     1381 agcggcaggc caatccaggc gagcaacact ccccccgcgg ctccgaatac cgcgagaacc
     1441 gccgcttcgg ccatctgcca acggatgagg ccgccgcggc cggctccaag tgcacgacgc
     1501 accgccacgt cccgcttccg gctctcggcg cgaacgagca agaggtttgc aacattcgcg
     1561 cacgcgacga gcagcacgat cccgacggtg ccaagcagaa tccagagcgg tctggcaatg
     1621 ctcccgatca gcacctcgtc gagcgcgcgg actaccggga cgtggcgctc gatgaactgc
     1681 cggtaggcgg gcggacctcc aaaccgttcg ggcagacggc gcgccagcgt cgccagttcc
     1741 gccgctaccg tctggtgatt ggctcccggt gccattcgtc cgacgaggac cagatcgaag
     1801 ttgccgggcg tgatcggccc ggtgacctgg tcatgcatcc acagcgacat gcgctcgttc
     1861 ggaaagccga attcggggcc catcacgccc accacggtcc tgagccggcc cgagacctcg
     1921 atcggcttgc cgagaatcga cgcgtcgccg ccgaaccacg tcgtccagag ccagtggctg
     1981 atgaccgcga cctcggcttc ctcgtccgcg tccgtcggca ggcgtccgat gatgggcttg
     2041 actcgcagtg tcgagaagac ggacggcgag gcggcggcga tgaagagacg ctcggactgc
     2101 gtcccggcgt gcacggtggt ctgcgccgtc tggagcatgc cgaggtcctc gagcgtcttc
     2161 gcgttctcgc gatactggac gtagaactcc gccccgacgc cgatctctcc acgcacatcg
     2221 gagccgggcg cggacgcgcg gatggcgacg agccggtccg gttcggagaa gccgaacggg
     2281 gcgagcacga cggcctcgac taccgtaaag atcgcggtgt tggcgccaat gctcacggcc
     2341 agcgtcacga cggcggcaca ggcgaatccc ggcgagcgtc tcaggctgcg cgaggcatcg
     2401 cgaaggtcgg tgaagagcgc ctcgaagagc gcgcgcaacc gcgcggggat ttccattcgg
     2461 cctcccgact cgccggcggc tgcgaggcta ctaaagacgg acccgaatca caacacgcct
     2521 cgcgaacacg tagaataacg gcggtaggca gcccacggtt cacagacgcc gtcggcggta
     2581 ggccggtcac ggttcacagc gggtccggag gtattcatgt tcgttcgtgt tggcgtgtcc
     2641 cttctcctgt tcgtcggact ctcggcgcct gcgttcgcgc aaacgccaga cggtgaagcc
     2701 gtcttcaagc aggcgtgcgc ctcgtgccac agggacggcc agacgcaggc cccgacgcgc
     2761 gagactcttc gccagatgac gccggaagcg atcctcaact cgctcacgct gggccgcatg
     2821 atggttcagg cgatctcgct cagcgagccc gagcagcgcg ccgtctcggt ggcgcttgcg
     2881 ggccggccct ttgcgccgcc ctcgccgccg gtcgtggtga accggtgtgc gacgtcgccg
     2941 ccgatgcggg atcccgcagg ccctggcggc tggagcggat ggggcaacgg cgtgtcgaat
     3001 acgcgctttc aatcggccgc caacggcggg ctgaccgccg ctgacctgcc gaagctgaag
     3061 ctcaagtggg ccttcggcta ctccatcgtc acatctgcac gcgcgcagcc gactctggcc
     3121 ggcgggcgcc tgttcgtcgc cagcgagaac ggcgaggtgc acgcgctgga tccgaagacc
     3181 gggtgcacgc actggacgtt caaggcgcag gccggggtca ggacggcgcc gtcggccgga
     3241 ccgtacaaga gcgcggcggg cgcggccggc tacgcggtgt acttcggcga cgcgcgcacg
     3301 aacgtgtacg cggtggacgc gaacacgggc cagcagatct ggatgcggaa ggtggacgag
     3361 caccgttcgg cctcgctcac cggcgccccg acggtctacg gcggcaaggt gttcgtgccc
     3421 gttcaagggc tgaacgaaga gggacagggg ggcaacgcga agtacgagtg ctgcacgttc
     3481 cgcggcagcg tgtccgcgct ggacgccagc acgggttccg tgctctggaa aacctacacc
     3541 atcgacgagc cgaagccccg ggcgaagaac aagaacggca ttcagcagtg gggccccgcc
     3601 ggcgccggca tctggtccgc gccgaccgtc gatccgaagc ggaacatggt ctacgtcgcc
     3661 acggggaaca actacgccga cccgccggcg cgtacgagcg acgcgatcgt ggcgatggac
     3721 atgaacagcg gaacgatcaa gtgggtcagc cagaccacgc ccaacgacaa ctggacgatg
     3781 gggtgccggc ccgagaaccc ggacaacccg aactgccccg ccaagatggg tccggaccac
     3841 gacttctcgg cctcgcccag ccttgcgacg gtgaacggcc gcgacctgct cgtgcttccg
     3901 cagaagtcgg ggatggcgta cgcgctcgac cccgacgcgg agggacagaa ggtgtgggag
     3961 ttccgcatcg gtcaaggcag cggcctgggc ggccaatggg gaggcgcggt agacgggcag
     4021 aacgcctact tcggcgtgtc cgatctgatg tcgccgaacc ccgggggcat tcgggccgtg
     4081 aacctggcca cggggcaact ggcctggagc gtcggcccgc aaacacggtt gtgcgatgcg
     4141 aagcgcccca cgtgccgcgc gtcgcagggc gcagccgtca cggccatccc gggagccgtg
     4201 ctctccggat cgctcgacgg cgggatgcgc gcgtactcgg ccacggacgg atcgatcatc
     4261 tggaccttcg acacgaacaa ggagttcgag acggtgaacg gcgtcaaggc gaacgggggc
     4321 ggcctcgaag gaccgggccc gatcgtcgcc ggcggcatgc tcttcttcaa ctccggctac
     4381 ggcggcttcg tcggcaaccc gggaaacgtg ctgctggcgt tcggaatcga ctaaagcagg
     4441 gggcaggggg cagttggcag ggggcgcagg gctcacagaa cgtcagaacc gcagccgcag
     4501 gccgccgccg acctgtgcgc cgccggcctt gacgtcggag gtgccgctcg gcagcgtcat
     4561 ctgaacggtg ccgctggaat actgcacgcc aacgcccacg cccacgttcg gcccgaagaa
     4621 gaaggcgaca tcgc
//
LOCUS       DBNGSH010000145         4004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_180041, whole genome shotgun sequence.
ACCESSION   DBNGSH010000145 DBNGSH010000000
VERSION     DBNGSH010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_180041"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="AC16CY_03415"
     CDS             complement(<1..275)
                     /locus_tag="AC16CY_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352855.1"
                     /translation="MPRTSLTAGAALVSLLAFGPSTELGAGPSTGLGASGLRAQLPLE
                     PRQDSGQGVTAAYEGWFKNADGSFSLLVGYFNRNQKQTLDIPAGPNNR"
     gene            complement(286..1632)
                     /locus_tag="AC16CY_03420"
     CDS             complement(286..1632)
                     /locus_tag="AC16CY_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352856.1"
                     /translation="MTGVRLALVVTALVAPASTALAETASRPVTFARDVAPILQQKCE
                     SCHRPDSVAPMSLITYTDVRPWAAAIKSRVASRQMPPWDIVKTVGIQQFKNDSSLSDE
                     QIDTIVGWVDAGAPLGDPKDMPPPRVWPKEETWAYAAELGPPDLIVKTPLYSMPARAQ
                     DAWYRPVVDTGVTEPRWVRAIEIRPSTLKGRRITHHAVARLRQDDGFDDAASRSTRGA
                     DGAGTPGLFMEWAVGKQGEMARPNTGKLLLPGSKFAWDVHYHAVGEAISDSVEMALYF
                     YPKGQEPKYRVVLGQFDAITGGRQNLDIPPNTVAIHQAFHVMKQAGRIENFQPHMHLR
                     GKAMSMEAILPDGSVQMLSHSNFQFNWHLNYIYTDEAAPLLPRGTVLRITAWHDNTSA
                     NKSNPDPNQWVGWGDRTVDEMAHAWVNVTYMSDEDYQAELVKRHSTPVGSSASARR"
     gene            complement(1751..2458)
                     /locus_tag="AC16CY_03425"
     CDS             complement(1751..2458)
                     /locus_tag="AC16CY_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HIR3352857.1"
                     /translation="MTNYAGLERQLTSVLHLSRRPVAVAFRDTPPDGVPALTGSQPSG
                     CSFWRLAAAGRVFYTVPSDHFNCPIGSYTHNITLPEERQVELKRTLGLMIDIGYIQME
                     EIPSVPRLPKTPPVTIYAPLAETPVDADVVVLAGTPSNLMLLHEAALRAGKNALPMLG
                     RPTCMAIPAALSGGIASSLGCIGNRVYTDVPDDQLYTVVSGPDLASIAGQLTTIVAAN
                     ETLSEYHRGRRASLASA"
     gene            complement(2549..3199)
                     /locus_tag="AC16CY_03430"
     CDS             complement(2549..3199)
                     /locus_tag="AC16CY_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3352858.1"
                     /translation="MDSVPSPAGTATFSLLETMRLEDGRVPRLERHLERMAIAARRFG
                     YGWAEPAVRGAVEATARSHASGSWRVRLLLSRDGVPSIECTGYEHSGGPWRVAFAAHP
                     VADARDPFVLNKTTHRAVYDTARSSHPDVDDVLLWNARGEVTEATIGNVVAEIDGVRY
                     TPPVSCGLLAGTFRAEQLEAGTIHERVLTKADVAAATRLWLINSVREWVDAELVAR"
     gene            3233..3916
                     /locus_tag="AC16CY_03435"
     CDS             3233..3916
                     /locus_tag="AC16CY_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3352859.1"
                     /translation="MRVLVVEDEQKVADAVREGLEDERYEVVVERTGEGAFFRVNTET
                     FDVIVLDLTLPGRDGLEILRALRQRRMDTPVLVLTARDSLEDRVTGLDSGADDYLVKP
                     FAFAELLARIRALVRRGRVAESPRLGAGDLEMDLVTRKVTRAGRPVDLTVREFELLEF
                     LMRYQSQVVSRETLARDVWKETARTTPLDNVIDVHIARLRRKVDLDQPVKLIHTVRGV
                     GFMLKEGEP"
BASE COUNT          634 a         1326 c         1361 g          683 t
ORIGIN      
        1 cgattgttcg gtcccgctgg aatgtcgagc gtctgcttct ggttccggtt gaagtatccg
       61 acgagcagcg aaaagctgcc gtcagcgttt ttgaaccagc cttcgtacgc cgcggtgacg
      121 ccctggcccg aatcctgtct cggctcgagc ggcagctgcg cgcgcaaccc gcttgccccg
      181 agccctgtcg aggggcctgc cccgagttct gtcgaggggc cgaacgccag gaggctgacg
      241 agagcggctc ccgcggtcag gctcgtgcgc ggcactcgcg gactcttatc gtcgcgcaga
      301 agccgaagag ccgacgggcg tcgaatggcg cttcaccagc tccgcctggt agtcctcgtc
      361 gctcatgtag gtgacgttca cccaggcgtg cgccatctcg tccaccgtgc ggtcgcccca
      421 gccgacccac tgattcggat ccgggttgct cttgttggcc gaggtgttgt cgtgccacgc
      481 ggtgatgcgc aggaccgttc ccctcggcag caggggcgcg gcctcgtcgg tgtagatgta
      541 gttgagatgc cagttgaact ggaagttgct gtggctcagc atctgcaccg aaccgtccgg
      601 gaggatcgcc tccatcgaca tcgccttgcc gcgcaggtgc atgtggggct ggaagttctc
      661 gatgcgcccc gcctgcttca tcacgtggaa ggcctggtgg atcgccacgg tgttgggcgg
      721 gatgtcgagg ttctgccggc cgcccgtgat ggcgtcgaac tgcccgagca cgacccggta
      781 cttcggctcc tgtccctttg gatagaagta cagcgccatc tcgaccgagt cgctgatggc
      841 ttcgccgacg gcgtgatagt ggacgtccca cgcgaacttc gatcccggca gcaggagctt
      901 cccggtgttc gggcgggcca tctccccttg cttgccgacg gcccactcca tgaacaggcc
      961 cggcgtgccg gctccgtccg cgcctcgcgt cgatcgcgac gcggcatcgt cgaatccgtc
     1021 gtcctggcgg agacgcgcga ccgcgtggtg cgtgatacgg cgccccttga gcgttgacgg
     1081 gcgaatctcg atcgcgcgca cccatcgcgg ctcggtgacg cccgtatcga cgacgggccg
     1141 gtaccaggca tcctgggcgc gggccggcat cgagtacaga ggcgtcttga caatcaggtc
     1201 gggcggcccg agctcggcgg cgtacgccca cgtttcttcc ttcggccaca cgcgcggcgg
     1261 cggcatgtcc ttcggatccc cgagcggcgc gcccgcatcg acccatccga cgatcgtgtc
     1321 gatctgctcg tcgctgagcg acgagtcgtt cttgaactgc tggatgccga cagtcttgac
     1381 gatgtcccac ggcggcatct gtcgcgaggc gacgcgcgac ttgatggctg cggcccacgg
     1441 gcgcacgtcg gtgtaggtga tgagcgacat cggcgcgacc gaatccggcc gatggcacga
     1501 ctcgcacttc tgctgcagga tgggcgcgac atccctggcg aaggtcaccg gccgtgacgc
     1561 ggtttcagcg agggcggttg acgcgggggc tacgagcgcg gtgacgacca gggccaaacg
     1621 cacgcctgtc atgggtcgct ccatttccgc gtgagcggga cgcgagcgga ttataacgcc
     1681 agataaatgg ggtcgggagt cattttcccc ggcgagcgtc tcccccggaa aatgactccc
     1741 gaccccattt tcacgccgag gccaggctgg cccgccggcc ccggtgatac tccgacagcg
     1801 tctcgttggc cgccacgatg gtcgtgagct ggccggcaat cgaggccaga tccggcccgg
     1861 acacgacggt atacagctgg tcgtccggga cgtccgtgta cacccggttc ccgatgcagc
     1921 caaggctcga cgcgatgcct ccggacagtg cggcgggaat ggccatgcac gtcggtctgc
     1981 cgagcatcgg cagcgcgttc ttccccgcgc ggagggctgc ctcgtgcagc agcatgaggt
     2041 tcgacggcgt gcccgccagg accaccacgt cggcatcgac cggtgtctcg gccagcggcg
     2101 catagattgt tacggggggt gtcttcggca gccggggcac gctcgggatt tcctccatct
     2161 ggatgtagcc gatgtcgatc atcaggccga gcgttctctt cagctcgacc tggcgctcct
     2221 cgggcagcgt gatgttgtgc gtgtagctgc cgatggggca gttgaagtga tccgacggga
     2281 ccgtgtagaa gacccgaccg gcggcggcca gacgccagaa gctgcagccg gagggttgcg
     2341 atcccgtcag cgccggaacg ccatccggcg gcgtgtcccg gaacgcgacc gcgacgggac
     2401 gacgagacaa gtgaagcacg ctggtgagct gacgttcgag ccccgcgtaa ttggtcatgg
     2461 aaggaacaat atcaaactcc caactcccaa ctcccaactc ccaactccca actcccaact
     2521 cccaactccc aactcccaac gcacatcgct atcgagccac gagttcggcg tcgacccact
     2581 cccggacgct gttgatcagc cagagccgcg ttgccgcagc cacgtccgcc ttcgtcagga
     2641 cgcgctcgtg gatagtgccc gcctcgagct gctcggcgcg gaaggtgccc gcaagcaggc
     2701 cgcacgatac cggcggcgtg tagcggacgc cgtcgatctc tgcgacgacg ttcccgatcg
     2761 tggcctcggt gacctcgcca cgagcgttcc acaacaacac gtcgtccaca tccgggtgtg
     2821 agctccgagc cgtgtcgtag acggcccggt gggtcgtctt gttcaggacg aacgggtcgc
     2881 gggcgtcggc cacggggtgc gccgcgaagg cgactcgcca cggaccgccg gaatgctcat
     2941 agcccgtgca ctcgatggaa ggcacgccgt cgcgcgagag cagcagcctc acgcgccagc
     3001 tcccggaggc gtgcgaccgg gccgtcgcct cgacggcgcc gcgaacggcc ggctccgccc
     3061 agccgtaccc gaaccggcgc gccgcgatcg ccattcgttc gagatggcgc tcgagacggg
     3121 gcacacgccc gtcctcgagg cgcatcgtct ctaggagcga aaacgtggcg gtcccggcgg
     3181 gggaggggac gctatccatg tccctttatt ctgacggaca atcggatcat ccatgcgggt
     3241 cctcgtcgtc gaagatgaac agaaggtggc ggatgccgtc cgcgaaggtc tcgaagacga
     3301 acgctacgag gtcgtggtcg agcggaccgg ggaaggggcg ttcttccgcg tcaacaccga
     3361 gacgttcgac gtcatcgtgc tggacctgac gctgccgggg cgcgacgggc tcgaaatcct
     3421 ccgggcgctg cgccagcgcc gcatggacac gccggtcctg gtgctgacgg cgcgcgattc
     3481 gctcgaagac cgcgtgacgg ggctcgacag cggcgccgac gactatctcg tcaagccgtt
     3541 cgccttcgcc gagctgctgg cccgcatccg cgcgctcgtc cggcgcgggc gcgtcgcgga
     3601 gtcgccgcgg ctcggcgccg gcgatctcga gatggatctc gtgacgcgca aggtgacgcg
     3661 cgcagggcgc cccgttgacc tgacggtccg ggagttcgag ttgctggagt tcctgatgcg
     3721 atatcagagc caggtcgttt cgcgcgagac gctggccagg gacgtctgga aggagacggc
     3781 gaggacgacg ccgctcgaca acgtgatcga cgtccacatc gcgcgcctgc gccgcaaggt
     3841 ggatctggat cagccggtga agctcattca caccgtgcgg ggcgtcggct tcatgctgaa
     3901 ggaaggcgag ccgtgacgcg cgtcggtccg ttcaggacgt ggtggcggtc gcacagcgta
     3961 cgcgtgcggc tgacgctgtg gtacgtcgcc gcgatcatgc tggt
//
LOCUS       DBNGSH010000146         5463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4595352, whole genome shotgun sequence.
ACCESSION   DBNGSH010000146 DBNGSH010000000
VERSION     DBNGSH010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4595352"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="AC16CY_03440"
     CDS             <1..994
                     /locus_tag="AC16CY_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3352860.1"
                     /translation="GYIDRERLRQGFSHLIVSDHILEQLGPAANANKAIFLYGPPGNG
                     KSVLAEGLGRTLGGDMYMPYAIEVEGHIITMYDPINHEPLERDEPASASIIATAAKDR
                     RWVRIRRPVVQVGGELTLDMLDLTFNSLAKFYEAPLQLKANGGVFLVDDFGRQRMRPE
                     DLLNRWIVPLESRVDYLTLHTGKKFQVPFDVLTVFATNLDPLSLADEAFLRRIPYKIE
                     VGDPTIEQFTRILEMNCRRRDLRFHPVIAAYLQRRHYAPTGRRLRSCHPRDLLDQVTA
                     LCRYRGIEPVLNRDMLDAACAAYFIDQNDEPAAEPAAEPVKRKSKPRRHQIEVR"
     gene            994..1401
                     /locus_tag="AC16CY_03445"
     CDS             994..1401
                     /locus_tag="AC16CY_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR3352861.1"
                     /translation="MAETTLALVNQRTDEALADRVEVAVTRKDRRKGLLGRDGFAVSE
                     ALVLAPCLAIHTMFMRFDIDAVFVDDDGRAVKVVENLAPWRIAVQPFAHAVVELPGGS
                     LSRRVAIGDRLYLVDEAGRRVGLSAADLKERVC"
     gene            1395..2732
                     /locus_tag="AC16CY_03450"
     CDS             1395..2732
                     /locus_tag="AC16CY_03450"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3352862.1"
                     /translation="MLTRPRVEKPVLDPQRYPVVLVTEEQLAELMRRPSMRQVNRHPR
                     APYTYVRRWTRWAAAAIVFAMVAAVGGLTWFFAPQPAMASFPWAATPRMPTITTGASF
                     SITIASLESGDEAGATATRVRAFGLPAFTRRSPGREQMYQAMVGPYVSLDEAERAQRR
                     LSGLGYRGGRIFVDESLRTGVHEQEPVSLGNKDIAALIVGAPERLSFVLELPEEPSQV
                     TSRRTTETAFDVDVSPLPGPMPAQQWRVPEGVHLLQAVTMEELSAPGGSKYLRARLAL
                     PEFAKANVRNEGRRVYVDLTWPVAEDDVRAPRRTVEEAAEPAAAFDSPRPKAPVSSPR
                     QDDQYREAMIPVHRRIAEMRPFLQSAAKSGSVEVLGALDQTLAALESSLTSMSVPASA
                     APQHQLLVAATRSARAGVDPAFTGDRQAHLQKAFDMFDGATAPAAPTATPGLQ"
     gene            complement(2713..3372)
                     /locus_tag="AC16CY_03455"
     CDS             complement(2713..3372)
                     /locus_tag="AC16CY_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="High-affinity nickel transporter"
                     /protein_id="HIR3352863.1"
                     /translation="MLVVVTGALAGFFHVLSGPDHLAAVAPLAVDRRDRGWIAGWTWG
                     IGHASGVTLVALLALLLRDALPPIDALSGWSERLVGVALIGIGFWALRRSARVRHSPH
                     QHGRLSHDHLHVQAGPRWLRHLGHAHASFFLGVLHGVAGSSHFFGVLPALGLPRDAAL
                     LYIGAFGVATVAAMTAFAALLGSIASHAPNAERAHRVMMTAAALLAIVVGGVWLTAAP
                     V"
     gene            3431..5203
                     /locus_tag="AC16CY_03460"
     CDS             3431..5203
                     /locus_tag="AC16CY_03460"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR3352864.1"
                     /translation="MTTLMARAALLASLAVAGCDRSPTRMLVTAAEQSGFARTGRYEE
                     VEALCTAFQRAWTDQVRCVEFGRSPENRPMLSLVASSDGALDPAAARARARPVVLMQG
                     GIHGGEIDGKDAGFLALRQILEGAAASGALSRVTLVFVPVFNVDGHERFGRWNRPNQT
                     GPEEMGWRTTAQNINLNRDYVKADAPEMRAMLRLLGEWDPILYADLHVTDGAQFEHDV
                     SITGGPIFDGDDDLRRVRRGLRDELIQRLSAQGSLPLDFYPSFVREDDPTSGFAVTAS
                     QARFADGYWTRRNRLSVLVETHSWKDYATRVRVTRNAIVAMVESAAANGAAWLAAARA
                     ADERSARIGGSSVALAYTATDHVKTIDFRGYAYTRQPSAISGSLSIRYDATRPQIWQV
                     PLRDEVRPTITAVAPRGGYIVPAAHAAWVGEKLALHGITSRVLTGVTGEADVEVFRAT
                     KTTLGSATFEGRVIRSVEGEWTAERRSVPAGSLFVPVAQAKSALVMTLLEPRGPDSLV
                     SWGFFNASFEPKEYMEAYVAEALGVTMLQTDPKAKDEFERRLREDPEFARSPSARLEF
                     FYRRHPSWDERLNLYPVMRVQSEP"
     gene            complement(5266..>5463)
                     /locus_tag="AC16CY_03465"
     CDS             complement(5266..>5463)
                     /locus_tag="AC16CY_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR3352865.1"
                     /translation="PGTFDTPLLAALPEAARASLAQQVPFPPRLGRPEEFASLVRHVF
                     ENEMLNGEVIRLDGAIRMAPK"
BASE COUNT          880 a         1858 c         1881 g          844 t
ORIGIN      
        1 cgggtacatc gatcgcgaac gcctgcgcca gggcttttcg catctgatcg tcagcgacca
       61 catcctggag cagctgggtc cggccgccaa cgcgaacaag gcgatcttcc tctacggacc
      121 tccgggaaac ggcaagagcg tgctggcgga gggcttgggc cgcacgctcg gcggcgacat
      181 gtacatgccg tacgccatcg aggtcgaggg tcacatcatc acgatgtacg acccgatcaa
      241 ccacgagccg ctcgagcgcg acgagcccgc gtcggccagc atcattgcca ccgccgcgaa
      301 ggatcggcgc tgggttcgca tccgccggcc cgtggtgcag gtcggcggcg agctgacgct
      361 ggacatgctg gacctcacct tcaacagcct ggccaagttc tacgaggccc cgctccagct
      421 gaaggccaac ggcggcgtgt tcctcgtgga cgacttcggc cgccagcgca tgcgtccgga
      481 agatctcctg aaccgctgga tcgtgccgct cgagagccgc gtggactacc tgacgctgca
      541 caccggaaag aaattccagg tgccgttcga cgtgctgacg gtgtttgcga ccaacctcga
      601 cccgctgtcg ctcgccgacg aagcgttcct gcgccgcatt ccctacaaga tcgaagtcgg
      661 cgacccgacc atcgagcagt tcacccgcat tctcgagatg aactgccgcc ggcgcgacct
      721 gcggttccac ccggtcatcg cggcgtacct gcagcgccgg cactacgcgc ccaccgggcg
      781 acggctgcgc tcctgccatc cgcgcgattt gctcgaccag gtgaccgcgt tgtgccgcta
      841 tcgcggaatc gagccggtgc tgaatcggga catgctcgac gccgcctgcg ccgcgtactt
      901 catcgaccag aacgacgagc cggccgctga gccggccgcc gagcccgtca agcgcaagtc
      961 gaagccacgc aggcaccaga tcgaggtgcg ctgatggcgg agacgaccct ggcgctggtc
     1021 aatcagcgga ccgacgaggc gctggcagac cgcgtcgagg tcgcggtgac gcggaaggat
     1081 cggcggaagg ggctcctcgg ccgtgatggc tttgccgtgt cggaggcgct cgtgctggcg
     1141 ccgtgcctcg ccatccacac gatgttcatg cggttcgaca tcgacgccgt cttcgttgac
     1201 gacgacgggc gcgcggtgaa ggtcgtcgag aacctggcgc cgtggcgcat cgccgtgcag
     1261 ccgttcgcgc acgccgtcgt cgagcttcca gggggcagcc tgagccgccg cgtcgccatc
     1321 ggcgatcgtc tctacctggt tgacgaggcc gggcgacgag tcggcctgtc ggctgccgac
     1381 ctgaaggagc gcgtgtgctg acccggccgc gcgtcgaaaa accggtcctc gatccgcagc
     1441 ggtacccggt cgtactggtg accgaggagc agctcgccga gctgatgcgg cgcccctcga
     1501 tgcgccaggt caaccggcat ccgcgcgccc cgtacacgta cgtccgacga tggacgcgct
     1561 gggctgcggc cgcgatcgtg tttgcgatgg ttgccgcggt tggcgggctc acctggttct
     1621 tcgcgccgca gccggcaatg gcgtcgttcc cgtgggccgc aacgccccgc atgccgacca
     1681 tcacgaccgg tgccagcttc agcatcacca ttgcctctct cgagagcggc gacgaagcag
     1741 gcgcgacggc cacgcgcgtc cgggcgttcg gccttccggc gttcacgcgc cgctcccctg
     1801 gcagggaaca gatgtatcag gcgatggtcg gcccgtacgt ctcgctcgac gaggccgagc
     1861 gggcgcagcg ccgcttgagt ggattgggtt atcgcggagg gcggatcttc gtcgacgagt
     1921 cgcttcgcac gggcgtgcac gagcaggagc cagtcagcct cggcaacaag gacatcgcgg
     1981 cgctgatcgt cggcgcgccg gagcgtctct cgttcgtgct ggagctgccg gaagagccca
     2041 gccaggtgac gtcccgccgc accaccgaga ccgccttcga cgttgacgtg agcccgctgc
     2101 cgggcccgat gcccgcgcaa cagtggagag tgcccgaggg cgtccacctg ctgcaggccg
     2161 tgacgatgga ggagctcagc gcgccgggag gcagcaagta tcttcgcgcg cgcctggcgc
     2221 tccccgagtt cgccaaggcc aacgtgcgca acgagggccg gcgcgtctac gtcgatctga
     2281 cctggcctgt cgcggaagac gacgtgagag cgccgcgacg caccgtcgaa gaggccgcgg
     2341 aacccgcggc ggcgttcgac agtccccggc cgaaggcgcc tgtgtcatcc cccaggcagg
     2401 atgaccagta ccgcgaggcg atgatccccg ttcaccggcg gattgcggag atgcggccct
     2461 tcctgcagtc ggccgcgaag tcgggctcgg tcgaggttct tggcgccctc gatcagacgc
     2521 tcgcggcgct cgagtcctcg ctgacgtcca tgagcgtgcc cgcctctgcc gcgccgcagc
     2581 accagctgct cgtcgcggcg acgcggtcgg cgcgtgccgg agtggacccc gcgttcacgg
     2641 gcgacagaca ggcgcacctg cagaaggcgt tcgacatgtt cgacggggcg acggcccccg
     2701 cggcgccgac ggctacaccg gggctgcagt aagccacacc cctcccacga caatcgcaag
     2761 gagcgcggcg gcggtcatca tcacccgatg cgcccgttcg gcgttcggtg cgtggctggc
     2821 gatggatccg aggagcgcgg caaaggccgt catcgcggcg acggtggcga cgccgaaagc
     2881 gccgatgtag agcagcgccg cgtccctcgg tagtccgagg gcggggagca cgccgaagaa
     2941 atgcgagctt ccagcgacac cgtgcagcac gcccaggaag aacgacgcat gcgcgtgccc
     3001 caggtgcctg agccatcgcg gccctgcctg cacgtgaagg tgatcgtgcg acaggcgtcc
     3061 gtgctggtga ggcgagtgcc ggacgcgcgc gctgcgccgc agcgcccaga agccgatgcc
     3121 gatgagcgcg acgccgacca ggcgctcgct ccatcccgag agcgcgtcga ttggcggcag
     3181 cgcgtcccgc aacaggagcg ccagcagggc caccagcgtc acgcccgagg catggccgat
     3241 gccccaggtc catcccgcga tccatcctct gtctcgacgg tctacggcaa gcggggccac
     3301 tgcggcgagg tgatcgggac cggacaggac atgaaagaat ccggcgagcg cgccggtgac
     3361 cacaacgagc atcgttccca tcattacacg ttttcgccgt caatcccggt gtggtttcat
     3421 gtggacgtcg atgacaaccc tcatggcgcg tgcagcgctg ctggcgtccc tggctgttgc
     3481 gggatgtgac agatcgccaa cccggatgct cgtgaccgcg gcggagcagt cggggtttgc
     3541 gcgaacgggc cgctacgagg aagtcgaggc gctgtgcacg gcgttccagc gggcgtggac
     3601 cgatcaggtc cgctgcgtgg agttcgggcg ctcgccggag aaccgtccga tgctctcgct
     3661 cgtcgcatcg agcgacgggg cactcgaccc ggccgcggca cgcgcacgcg cgcgccccgt
     3721 ggtgctgatg caggggggca ttcatggcgg cgagatcgac ggcaaggacg ccgggtttct
     3781 ggcgctgaga cagatcctcg agggcgccgc ggcgtcgggc gcgctctcgc gcgtcacgct
     3841 cgtgttcgtt ccagtgttca acgtcgatgg ccacgagcgc ttcggacgct ggaatcgtcc
     3901 gaaccagaca gggcccgagg agatgggctg gcggacgacc gcgcagaaca tcaacctcaa
     3961 tcgcgactac gtgaaagccg acgcgccgga gatgcgggcc atgctgcgtc tgctgggaga
     4021 gtgggatccg attctgtatg cggatcttca cgtgacggac ggtgcgcagt tcgagcacga
     4081 cgtctccatc accggcgggc ccatcttcga cggcgacgac gaccttcggc gtgtgcgccg
     4141 cggcctccgc gacgagctga tacagcgtct gtcggctcaa ggctcgctgc cgctcgactt
     4201 ctatccgtcc ttcgtcagag aggacgatcc aacctcgggg tttgccgtca cggcctcaca
     4261 ggcacgcttt gccgacggct actggacgcg gcgaaatcgt ctgtcagtgc tcgtcgagac
     4321 tcattcgtgg aaggactatg ccacccgcgt tcgcgtcacg cgcaacgcga tcgtcgcgat
     4381 ggtcgagtcg gccgctgcga acggtgccgc atggctggcc gccgcccgcg cggccgacga
     4441 acggtccgcc cgcatcggcg ggtccagcgt tgcactggcc tataccgcca ccgatcacgt
     4501 caagacgatc gacttccgcg gctacgccta tacccgccag ccgtcggcga tctccggaag
     4561 tctgagcatt cgctacgacg cgacgcggcc gcaaatctgg caggtgccgc tgcgcgacga
     4621 ggtacggccg acaatcaccg cggtcgctcc acgaggcggc tacatcgttc cagctgcgca
     4681 cgcggcgtgg gttggcgaga agctggcctt gcacggcatc acgagccgtg tcctgaccgg
     4741 cgtgaccggc gaagccgacg tcgaggtgtt tcgcgccacg aagacgacgc tcggctcggc
     4801 cacgttcgag ggccgcgtga tccgctcggt cgagggcgag tggaccgccg aacgacgctc
     4861 cgtgccggcc ggctcgttgt tcgttcctgt cgcccaggcg aagtcggcgc tcgtgatgac
     4921 gctgctcgag ccgcgagggc cagactcatt ggtcagttgg ggattcttca acgcctcgtt
     4981 cgagcccaag gagtacatgg aggcgtatgt cgccgaagcg ctcggcgtca cgatgctcca
     5041 gaccgatccc aaagcgaagg acgaattcga gcgccggctg cgcgaggacc ccgagtttgc
     5101 ccggagcccc agcgcgcggc tcgagttctt ctatcgccgc cacccgtcgt gggacgagcg
     5161 gctcaacctg tatccggtga tgcgggtcca gagcgaaccc tgaggcttga gccctgaggc
     5221 ctgggcccga accctgaggc ctgggccctg agccctgggg cctgcctatt tgggagccat
     5281 ccggatcgcg ccgtcgagcc ggatgacctc gccgttcagc atctcgttct cgaagacgtg
     5341 cctgacgagt gacgcgaact cctccgggcg tccgaggcgt gggggaaacg ggacctgctg
     5401 cgccagcgac gcgcgcgcgg cttcgggaag cgcggcgagc aggggcgtgt cgaacgtccc
     5461 ggg
//
LOCUS       DBNGSH010000147         4324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_180174, whole genome shotgun sequence.
ACCESSION   DBNGSH010000147 DBNGSH010000000
VERSION     DBNGSH010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_180174"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="AC16CY_03470"
     CDS             <1..260
                     /locus_tag="AC16CY_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352866.1"
                     /translation="LGNFTGFRQGSPDYERVINIVHDAAIKAGVRLCGPLAWRDRPDF
                     TCFQAGTEVAAIRRGVAAELGNLTNTQAVPEVGPFAKPAAR"
     gene            286..1359
                     /locus_tag="AC16CY_03475"
     CDS             286..1359
                     /locus_tag="AC16CY_03475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3352867.1"
                     /translation="MKRLSVTLAIGVMLVIAAAVREPLRDTAAAQSRQPAGTAHTGKA
                     FRFNKVREGVYHAVGTGSLAVVGNSAFIVNDNDVIVVDDHVSPAAAWVLLEEIKEVTN
                     KPVTTVINTHFHFDHAHGNQIFEPGVQIIGHAFTRQMLLGNSTGMPLYQNYVGTMPGQ
                     IDTLRKRVASEADAAAKAKLQSQLQVLENNFASQKELKPTPPNVTLTTAMSLFRGNRD
                     IQVRFLGRGHTAGDVVVFLPNEKVVITGDFLTAGLSNMSDSYPEEWVASLDALKKLDF
                     DTVLPGHGEAFTDKAKIDYFQAYLRDVWSEVGRLKREGASAAEAAKRADLTKHKDRLP
                     IQGPGVPPIAATRIYELLDAEKR"
     gene            1451..2236
                     /locus_tag="AC16CY_03480"
     CDS             1451..2236
                     /locus_tag="AC16CY_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352868.1"
                     /translation="MKTLVRSSRGHLKYTGKITRESFSSWAESPAGRQALQAVAARVR
                     FALFGRMRAARRKIWRELIEAARAGTAVEVLQREADAYLSRLDTFVYADALPSVMVDL
                     HRLIVVPRVFVNGEAHRRIERALRAQPLFGGLSGGDSLRRWFAFGLIDGLAGAVTDAR
                     PSVRQPLPAGDGWIVVGMDEDFQWHIPFDGPPWPGHYFVLELTRRPLTRAAKRDAAEA
                     IARFGESLRSLSVLDRAEIMRRAAVSLDQLLGPRTRAVMSRSA"
     gene            complement(2249..2887)
                     /locus_tag="AC16CY_03485"
     CDS             complement(2249..2887)
                     /locus_tag="AC16CY_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352869.1"
                     /translation="MFKVIAGAVGVILALASVAAQEREYQPTCKMCPGTYIPSSEIDA
                     YVKRAIANQLTDQQVRQVDIGKANVGVAVVHRGRLNNPEASVAEHDLVSEVYHVISGS
                     ATLVLGPDLVGKERRPSTQTTVRLLNGPGNNAKSIRNGVAHQIKTGDVVVIPAGTGHQ
                     FTRIDDHVTYLMIRVDPDKVTPHKTEADSLADLKTDGRASAGGGAGAAPAPR"
     gene            complement(2991..3575)
                     /locus_tag="AC16CY_03490"
     CDS             complement(2991..3575)
                     /locus_tag="AC16CY_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352870.1"
                     /translation="MRRAAILLATFLILPPSAASAGQVLCRNEADDIAALQMFDRAVN
                     AYVEMTRRVEVPLSPWIASDLEAMAAANDALARAIAAERPDARQGDIFGPEVADFFRV
                     RIDASGALKGRQYTLARCGEEAEDAPCVRPPLVHSRLAWMWSAPIGDALARALPPLPL
                     ELQYRLSPDGSVLLLVDVRAALVIDLMDVAPASS"
     gene            complement(3662..>4324)
                     /locus_tag="AC16CY_03495"
     CDS             complement(3662..>4324)
                     /locus_tag="AC16CY_03495"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3352871.1"
                     /translation="LHFVGPCVTVFGSARYGESHPHYALGRHVGASLARMGFTVMTGG
                     GPGLMEAANRGAREAGGRSVGCNIELPTEQEPNPYLDRWVTCRYFFVRKVLLFKYSYA
                     FVGLPGGLGTLDELFEALVLIQTGKIDNFPVVLFGTAYWRPLQDLLHHLAREGTISES
                     DLTLLLVTDSVEDALAHIRRHAVEQFGLRLKPSKLFRERRQGGTASTGAFSAGARTNP
                     ER"
BASE COUNT          746 a         1429 c         1430 g          719 t
ORIGIN      
        1 acctggggaa tttcaccggg ttcaggcagg gttcacccga ttacgaacgc gtgatcaaca
       61 tcgtgcacga cgccgccatc aaggcggggg tgcgtctgtg cggtccgctc gcgtggcggg
      121 atcgtcccga cttcacctgc ttccaggcgg gcaccgaggt cgcggcgatc cggcggggcg
      181 tggcagcgga gctgggtaat ctgacgaaca cgcaagcggt tccggaggtt ggtccgttcg
      241 cgaagccggc cgcgcgttga cacctgcaga tcgatcggag aacggatgaa acgactgagc
      301 gtgacgcttg cgatcggtgt gatgctcgtc atcgcggcgg cggtgcgcga gcctttacgc
      361 gacaccgctg ctgcgcaatc caggcagccc gccggcacgg cccataccgg caaggccttc
      421 cggttcaaca aggtcagaga aggggtctat cacgccgtcg gcaccggttc gctggccgtc
      481 gtcggcaact ccgccttcat cgtcaacgac aacgacgtga tcgtcgtcga cgatcacgtc
      541 tccccggccg cggcctgggt gctgctggaa gagatcaagg aggtcacgaa caagcccgtg
      601 accacggtga tcaacacgca cttccacttc gaccacgcac acggcaacca gatcttcgag
      661 ccgggcgtgc agatcatcgg gcacgcattc acgcgtcaga tgctgctcgg caactcgacc
      721 gggatgccgc tgtaccagaa ctatgtcggg acgatgcccg gccagatcga cacgctgaga
      781 aaacgtgtcg cctcggaggc cgacgccgcc gcgaaggcca agttgcagtc gcagctgcag
      841 gtcctggaga acaatttcgc gtcgcagaag gagctgaagc cgactccgcc gaatgtgact
      901 ctcaccaccg cgatgagtct ctttcgaggg aatcgtgaca tccaggttcg cttcctcggc
      961 cggggtcaca cggccggcga cgtcgtcgtc ttcctcccga acgagaaggt cgtcatcacc
     1021 ggagattttc tcaccgcagg cctctcgaac atgagcgatt cgtaccccga ggagtgggtc
     1081 gcctccctcg atgcgctgaa gaagctcgat ttcgatactg ttctcccggg acacggagag
     1141 gcattcaccg acaaggcgaa gatcgactac ttccaggcgt acctgcggga tgtctggagc
     1201 gaggtgggcc ggctcaaacg tgaaggcgcc tcggcggcag aggccgccaa gcgcgcggac
     1261 ctcacgaaac acaaggaccg cctgcccatc caggggcccg gcgtccctcc gatcgctgcc
     1321 acgcgcatct acgagctgct cgacgcggaa aagcgctgac gccgttggca cggcgttcgc
     1381 tatcgctgat cacgtcgggc cacgttctcc ccgcccgggc ccgtatccgc gacgtgacgg
     1441 cattacagaa atgaagacgc tcgtacgttc ttcgcgcggg cacctgaaat acacggggaa
     1501 gatcacgcgc gaatcgtttt cgtcgtgggc cgaatcgcca gccggccggc aggcactcca
     1561 ggctgtggcc gcccgtgtgc gcttcgcgct gttcggccgg atgcgcgcag cccgcaggaa
     1621 gatctggcgc gagctgatcg aggcggctcg cgccggcacg gctgtcgagg tcctgcagcg
     1681 cgaggccgac gcgtacctct ctcgtctcga cacgttcgtg tacgccgacg cgctgccgag
     1741 cgtcatggtc gatcttcacc gtctcatcgt agtgccacgc gtgttcgtca acggtgaagc
     1801 gcatcggcgc atcgagcgtg cccttcgcgc acagccactc ttcggtggcc tcagcggcgg
     1861 cgattcgttg cggcgctggt tcgcctttgg cctgatcgac gggctcgcgg gcgccgtcac
     1921 cgacgcgcgg ccgtcggtcc gtcagccgtt accggccggc gacggctgga tcgttgtcgg
     1981 gatggacgaa gacttccagt ggcatatccc cttcgacggc ccgccatggc cgggccacta
     2041 tttcgtgctc gagctcacac gacgcccgct gacgcgcgca gcgaagagag acgcggccga
     2101 ggcgatcgcc cggttcgggg aatcgctccg gtctctatcc gtcctcgatc gggccgaaat
     2161 catgagacgg gcggctgtgt cactcgatca gctgctcggc ccgcgcacac gcgccgtgat
     2221 gtcccggtcg gcctgaaaat cgaggtcttc agcgcggagc cggcgcggcc ccggcgccgc
     2281 cgcccgccga tgcgcggccg tctgtcttca ggtccgcgag cgagtcggct tcggtcttgt
     2341 gcggcgtgac cttgtcggga tccacacgaa tcatcagata ggtgacgtgg tcgtcgatcc
     2401 tcgtgaattg atggcccgta ccggccggaa tgacgacgac gtccccggtc ttgatctggt
     2461 gcgccacgcc gttacggatc gacttggcgt tgttgcccgg gccattgagc agccgaaccg
     2521 tcgtctgagt ggaggggcgg cgctccttgc cgacgagatc cggccccaga acgagagtcg
     2581 ccgagcctga gatgacgtga tagacctcgc tgacgaggtc gtgctcggcc acggacgctt
     2641 cggggttgtt cagcctgccg cggtggacga cggccacgcc gacgttggcc ttgccgatgt
     2701 ccacctggcg gacctgctgg tcggtgagct gattggcgat ggcgcgcttg acgtacgcgt
     2761 cgatctcgga gctcggaatg taagtgcccg ggcacatctt gcaggtcggc tgatactcgc
     2821 gttcctgagc cgcaaccgac gcgagggcaa ggatcactcc gacggcaccc gcgatgacct
     2881 tgaacatgac gtgcgcctcc ccgcgcaggg gggattctct ccgacaacgc gcgtcacgag
     2941 caaacgcctc gcgggagccc gtgtccagcg gcgcgaggat gggcgctgaa tcacgaggac
     3001 gccggcgcca cgtccatcag gtcgatcacg agcgcggccc ggacatccac gaggagcagc
     3061 acgctgccgt cgggcgacaa ccggtactga agctccagcg ggagcggtgg cagcgcgcga
     3121 gcgagcgcgt ccccgatcgg cgcgctccac atccatgcca gccggctgtg caccagcggc
     3181 ggacgcacgc aaggcgcgtc ctcggcttct tcaccgcagc gcgcaagagt gtattgacgt
     3241 cctttgagcg cacccgaggc atcgatccgc acgcggaaga agtcggcgac ttcagggccg
     3301 aagatgtcgc cctgccgcgc atccggccgc tcggccgcga tcgcccgcgc cagcgcgtcg
     3361 ttcgcggcgg ccatcgcttc cagatcggag gcaatccagg gtgacaacgg cacctcgacg
     3421 cggcgagtca tctcgacgta cgcgttgacc gcgcggtcga acatctgcag ggcggcgatg
     3481 tcgtcggctt cgtttcgaca cagcacctgg ccggcggatg cggccgatgg cggaagtatc
     3541 aggaaggtcg caagcaggat ggctgcgcgt cgcatggtcc acctccggac gacgggtgaa
     3601 ccgcagtaat cgtgccagca ggccgcgacc aaccagcgcc atcattcggc gcgggtccga
     3661 ttcaccgctc gggattcgtt cgcgcgcccg cggaaaaagc cccggtggag gccgtccctc
     3721 cctgtcggcg ctctcggaac agtttcgacg gtttgagacg caacccgaac tgttcgacgg
     3781 cgtggcggcg tatgtgggca agcgcgtctt cgactgaatc ggtcacgagc agcagcgtga
     3841 gatcggactc gctgatcgtc ccctcgcggg cgaggtggtg aagcaggtcc tgcagcggac
     3901 gccagtaggc ggtgccgaac agcaccacgg ggaagttgtc gatcttccct gtctgaatca
     3961 gcaccagggc ttcgaagagc tcgtcgagcg tgcccaggcc tcccggaagt ccgacgaatg
     4021 cgtacgagta cttgaagagc agcaccttac gcacgaagaa gtagcggcac gtcacccatc
     4081 gatcgaggta ggggttgggc tcctgctcgg tgggcagctc gatgttgcaa cccaccgatc
     4141 ggcctcccgc ctcgcgcgca cccctgttgg cagcctccat cagccccggc ccgcctcccg
     4201 tcatcacggt gaaccccatg cgggcgaggc tggcgcctac gtgccggccg agggcgtaat
     4261 gcggatgcga ctctccgtac cgcgccgagc cgaacacggt gacgcagggc ccgacgaagt
     4321 gcag
//
LOCUS       DBNGSH010000148         1603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_859644, whole genome shotgun sequence.
ACCESSION   DBNGSH010000148 DBNGSH010000000
VERSION     DBNGSH010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_859644"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..845)
                     /locus_tag="AC16CY_03500"
     CDS             complement(270..845)
                     /locus_tag="AC16CY_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352872.1"
                     /translation="MRAGILSASICFVLLVVAYAASLAPRAQTPQQTPAAGQAQGQPA
                     PPPPQPGHPSGQLVLWGDVALFSKPDDPDNCILTNRYKRGQRVGFRMTAIDGGTGEVE
                     NTATLVAHVTYAGKTVDAPMRFRGAAGRGAPAPRGYLASPYNLWTGSWTVPDDAPVGR
                     ISYTVTATDKFGRKATFEPFAAEASQLVIVP"
     gene            953..1603
                     /locus_tag="AC16CY_03505"
     CDS             953..1603
                     /locus_tag="AC16CY_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352873.1"
                     /translation="MSSRPNRRSFMTAVGSLAGLAAMPPVAEAQGPAASATPADLGWF
                     DSFKGRHRQVYDYGGYNLVSTDPPVPALRFPRNYLDSVRDTLRLEFPEVNTAVGVSLP
                     SYPLNATDAVWQKYGLGERFKIVDPATKKPAVRNIFLDGGEFSVKALQARGTVFWQCN
                     YALDALIQQLATSTGRPVAEVRADLLAGLNPGVRLVPSHVLALGLAQQRGFSYVRA"
BASE COUNT          264 a          512 c          553 g          274 t
ORIGIN      
        1 tgaccaggcg cggctggtcg cgcacgatgt tcaaggtggg ggacgaggtg acggtgacgt
       61 tgcagcccgt gaagagcggc gcgcccgtgg gacgcgttcg cagcgtgatg ctcgccaacg
      121 gacaaacagt cggcacggga ggcggacccc cgcccgcgca accggccaaa tagcagctca
      181 cccaggaacg aaccacagag acacggaggc acggagcaat caatcactcc gtgtcttcgt
      241 gactccgtgg tttgtgatcc gaatcggttt caggggacga tgacgagctg cgatgcttcg
      301 gcggcgaagg gctcgaacgt cgcctttcgc ccgaacttgt ccgttgccgt caccgtgtag
      361 ctgattctgc cgacgggggc atcgtcaggc acggtccacg aacccgtcca caagttgtaa
      421 ggagacgcca ggtacccgcg gggcgcgggc gcgccccgtc ccgccgcgcc gcggaaccgc
      481 atgggcgcat ccacggtctt gccggcgtag gtcacatggg ccacgagcgt cgccgtgttt
      541 tcgacttcgc cggttccccc gtcgatggcc gtcatccgga atccgacgcg ctgtcctctc
      601 ttgtagcgat tcgtcaggat gcagttgtcc ggatcgtccg gtttgctgaa gagcgcgacg
      661 tcaccccaga gcacgagctg gccggaaggg tgcccgggct gcggcggcgg cggggctggc
      721 tgaccttgcg cctgtcccgc tgcgggcgtc tgctgcggcg tctgggcgcg cggagcaagc
      781 gaggcggcat acgcgacgac cagcaggacg aagcagatgg acgccgagag gatgccggct
      841 ctcatgctgg tgtcgactat aactcatggc gggccgggag gcgagagtcc gggcgcgagg
      901 ctcattgatt cggggcgtat acaattccgt cgatcgtcga aaggagctcg acatgtcttc
      961 cagaccaaac cgccgatcct tcatgacggc ggtcggctcg cttgctggac tggcggcgat
     1021 gccacccgtc gccgaggccc aaggtccagc ggcatccgca acgcccgccg atctcgggtg
     1081 gttcgacagc ttcaaggggc gtcacaggca ggtgtacgat tacggcggct acaacctggt
     1141 ctcgaccgat ccgccggtgc cggcgctccg gtttccacgc aattacttag acagcgtccg
     1201 cgacactctc cggctggagt ttcccgaggt caacaccgcg gtcggggtct cgcttccgtc
     1261 gtatccgttg aacgccaccg acgccgtctg gcagaagtac gggcttggcg aacggttcaa
     1321 gatcgtcgat ccagcgacca agaagcccgc cgtgcggaac atcttcctcg acggggggga
     1381 gttcagcgtg aaggcgctgc aggcgcgcgg caccgtgttc tggcagtgca attacgcact
     1441 ggacgccctg attcagcagc tggcgacttc cacgggccgg cctgtggccg aggtgcgagc
     1501 cgatctgctc gctgggctga accccggcgt gcggctcgtc ccgtcgcacg tgctggcgct
     1561 cgggctggcg cagcagcggg gcttctcgta cgtgcgcgcg tga
//
LOCUS       DBNGSH010000149         3042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2727344, whole genome shotgun sequence.
ACCESSION   DBNGSH010000149 DBNGSH010000000
VERSION     DBNGSH010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2727344"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="AC16CY_03510"
     CDS             <1..1788
                     /locus_tag="AC16CY_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352874.1"
                     /translation="PRVRQLGILIRPHPENLQPWQRFDFSGLHDVVLWPRGGANPVDQ
                     GSKNDYYDSMYHAAAAVGVNTSAQIEAGIVGRPVFSVRVPEYAGTQEGTLHFHYLLNE
                     NGGLLHMADSLDAHTADLVKAVVRTEEDDRRLRGFVEGFVRPRGLGQPATPILADAIE
                     ELARRPQPAPERLTVGLHLVRLVLHPVGIVMKLVRYVSRLMRKRERQMRPLTVGTYVS
                     KRFATVIANIFRWAPAKRFAKRYIVPRVLPDMMTGDMPTQEMNAVPKILQRMSASHKH
                     VVVGPWLSEVGFELLYWIPFLNWVKTYRPFDPERLLVVSRGGAGIWYKNITPHYLDLF
                     DFYTPEEFRAKNAGRLRENMQKHLALTDFDREILKVAYQHLGSKSVELLHPMYMYRLF
                     YAYWKSRTSISLVDTFASFERLPALDTSDIARELPRDYTAVRFYFNESFPDTEDNKLF
                     VIRLLQTLTEAADVVLLNPDLHIDDHWEMPIRTNPRIHTVDHLMTPRNNLEIQSKVIR
                     GARAFVGTYGGLSYLAPMYGVTSLAFFSHREKFSPQHLELARRVFGSFKRGSYVVLDT
                     SDIDVLGLAFGDRGQLTQAVARTEAVIDG"
     gene            1785..2984
                     /locus_tag="AC16CY_03515"
     CDS             1785..2984
                     /locus_tag="AC16CY_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352875.1"
                     /translation="MSVRGVIRRWLGVDDLPERAARDAARETRDMRAVRDLLRQVDEL
                     RGLVHQQTAFVEEALHRAGWQSDQEAAQQGALHRALRAARLPGEVIVGPWTGEVGFEL
                     LYWIPFLGWLASQGVSGSRMTVLSRGGAGVWYRHLTTRYLDVLDLMTPDDFRERTAGK
                     KKQYDDKRDLDVELIEKARARLSLPDALVVHPSAMFRLFRGLWSKQATVELVESFASY
                     RPLEPPPAADASPAGLPSNYVVAKFYFSQAFPETAENRAFVAEMLRTVSAQVPVVVLS
                     TSVLLDEHHDVQVARGRGLIVVDTHTVPQRNLELQTRLIAGARGFIGTYGGFSYLAPF
                     YGVPSLSFFSRRNAFEPHHLELANRVFDKLLPGGFLAIDRRAANLVAPSLSRWAEAAR
                     PLASAEA"
BASE COUNT          487 a         1014 c         1018 g          523 t
ORIGIN      
        1 ccgcgcgtcc gccagttggg gattctcatc cgtccgcatc cggagaatct tcagccgtgg
       61 cagcgcttcg acttctcggg cctgcacgac gtcgtgctct ggccgcgcgg cggtgcgaac
      121 ccggtcgatc agggctcgaa gaacgactac tacgactcga tgtaccacgc ggcggccgcg
      181 gtgggcgtca ataccagcgc gcagatcgag gcgggcatcg tggggcggcc cgtgttctcg
      241 gtgcgagtcc ccgagtacgc cgggacgcag gaaggcaccc tgcactttca ctacctgttg
      301 aacgagaacg gcggcctgct ccacatggcc gactcgctcg acgcgcatac cgccgatctc
      361 gtgaaggccg tcgtgcgaac cgaggaggac gaccgccggc tgcgcggctt cgtcgaaggg
      421 ttcgtgcggc cgcgcgggct cggccagccg gccacgccga ttctggccga cgcgatcgag
      481 gagttggccc ggcggcctca gccggctccg gagcggttga cggtgggcct ccatctcgtg
      541 cgtctggtac tgcacccggt cggcatcgtc atgaagctgg tccggtacgt ctcgcggctg
      601 atgcgcaagc gcgaacgaca gatgaggccg ctgaccgtcg gcacctatgt ctccaagcgc
      661 ttcgccaccg tgatcgcgaa catcttccgg tgggcgcccg caaagaggtt cgcgaagcgg
      721 tacatcgtgc cacgcgtcct gcccgacatg atgaccggcg acatgcccac gcaggagatg
      781 aacgcggtcc cgaagatcct ccagcggatg tccgccagcc acaagcacgt cgtcgtcggg
      841 ccctggctca gcgaggtcgg gttcgagctg ctgtactgga tcccgttcct gaactgggtg
      901 aagacctacc gcccgttcga tcccgagcgg ctgctggtcg tctcgcgagg cggcgccggc
      961 atctggtaca agaacatcac accgcactac ctcgatctgt tcgacttcta cacccccgag
     1021 gagttccggg cgaagaacgc gggccggctg cgcgagaaca tgcagaagca cctcgcgctg
     1081 accgacttcg atcgggagat cctcaaggtg gcctaccagc acctgggaag caagtcggtc
     1141 gagctgctgc acccgatgta catgtaccgg ctcttctacg cgtactggaa gagccggacg
     1201 tcgatcagcc tggttgacac gtttgcgtcg ttcgagcgcc tgccggcgct cgacacgagc
     1261 gacatcgcgc gcgagctgcc gcgcgactac accgccgtgc ggttctactt caacgagtcg
     1321 tttccggaca ccgaggacaa caagcttttc gtcatccggc tgctccagac attgacggag
     1381 gcggccgacg tcgtgctgct caatccggat ctgcacatcg acgaccactg ggagatgccg
     1441 atccgcacga atccgcgcat tcacacggtt gaccacctga tgacgccgcg gaacaacctg
     1501 gagattcagt cgaaagtcat ccggggcgcg cgcgcgttcg tggggaccta cggcggcctc
     1561 tcgtatctgg cgccgatgta cggcgtgacg tcgctcgcat tcttctcgca cagggagaaa
     1621 ttctcgcctc agcacctgga gctggccagg cgggtgttcg gcagcttcaa gcgcggatcc
     1681 tacgtggtgc tcgacacgag cgacatcgac gtgctcggcc tggccttcgg cgatcgcggg
     1741 caattgacgc aggccgtcgc caggaccgag gcggtgatcg acggatgagc gtgcgcggtg
     1801 tcatccggcg ctggctgggg gttgacgacc tgcccgagcg tgcggcacgc gacgccgcgc
     1861 gcgagacccg ggacatgcgc gccgtacgcg atctcctccg ccaggtggac gaactccggg
     1921 gcctcgtcca tcagcagacc gcgttcgtcg aggaggccct gcacagggcg gggtggcaga
     1981 gcgatcagga ggcggcgcag cagggggcgc tgcaccgcgc gctgcgcgcg gcgcgcctgc
     2041 cgggcgaggt catcgtcggc ccatggacgg gcgaggtcgg cttcgagctc ctgtactgga
     2101 ttccctttct gggttggctg gccagccagg gcgtcagcgg ttcccgaatg acggtcctgt
     2161 cgcgcggagg cgccggcgtc tggtaccggc atctgacgac ccgctacctg gacgtcctcg
     2221 acctgatgac gccggacgac ttccgcgagc gcacggcggg caagaagaag cagtacgacg
     2281 acaagcgcga tctcgacgtg gagctcatcg agaaagcccg tgcgaggctg tcgcttcccg
     2341 acgcgctcgt cgtccacccc tcggcgatgt tccggctctt ccgcgggttg tggagcaagc
     2401 aggcgacggt cgaactggtc gagtcgtttg cgtcgtaccg tccgctcgag ccgccgccgg
     2461 ccgccgatgc gtcccccgcg gggctgcctt cgaattacgt cgtcgcgaag ttctatttca
     2521 gccaggcgtt tccagagacg gcggagaaca gggcgttcgt cgcggagatg cttcgaacgg
     2581 tcagcgcgca ggtgcccgtc gtcgtgctga gcacgtcggt gctgctcgac gagcatcacg
     2641 acgtccaggt ggcccgcggc cgcggactga tcgtcgtgga cacgcacacc gtgccccagc
     2701 gcaacctcga gctgcagacg cggctcattg cgggcgcccg cgggttcatc ggcacctacg
     2761 gcgggttctc gtacctggcg ccgttctacg gagtgccgtc gctgtcgttc ttttcccggc
     2821 ggaacgcgtt cgagccgcac cacctggagc tcgccaatcg cgtcttcgac aagctgctgc
     2881 cgggaggctt tctggcgatc gacaggcgtg ccgcaaatct cgtcgcgccg tcgctcagcc
     2941 gctgggctga agcggcgcgg ccgctcgcat ccgcagaagc atgaccggcc tcaggttcct
     3001 gttcgtgatg cagtacccgg ggtacctgcg ctacttcgac tc
//
LOCUS       DBNGSH010000150         2929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1368931, whole genome shotgun sequence.
ACCESSION   DBNGSH010000150 DBNGSH010000000
VERSION     DBNGSH010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1368931"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="AC16CY_03520"
     CDS             <1..594
                     /locus_tag="AC16CY_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR3352876.1"
                     /translation="DAARHVETRPIKGTRPRGVGPEHDAALGQALAESAKDRAENLMI
                     VDLMRNDLSRVCAPGTVRVSELFSLEHYATVHHLVSTVVGELAPGADAIDLLRASFPG
                     GSITGAPKLRAMEVIAELEPSRRGVYCGAIGYWSLSGELDTSIAIRTALVQGDRIYFS
                     AGGGIVADSDPEEEYRETLDKARAMIDTLALGALSRL"
     gene            complement(863..1492)
                     /locus_tag="AC16CY_03525"
     CDS             complement(863..1492)
                     /locus_tag="AC16CY_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352877.1"
                     /translation="MFKHVVLAALIASLAVPLSAQAPAGWKMRLDKSTSAQDPDDKSG
                     VTFRAAGKGLQVAGGPAGVYWNPSTTAAGNYTAKGTFTLLKPSSHTNYYGIVFGGQGL
                     DGADQRYTYFTVAQDGTFLIKQRTGAATRDVKGRTPSPAVTRPDASGRSVNALEVRVS
                     GDTVTYVVNGMVVHTTPKAELNAPTDGVVGFRVNHVTEVQVDGFEVQKS"
     gene            complement(1612..2793)
                     /locus_tag="AC16CY_03530"
     CDS             complement(1612..2793)
                     /locus_tag="AC16CY_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352878.1"
                     /translation="MSRSAATARPDVAPAEAAASLAAAAAPRHSGYLFHPLTDFLMAG
                     GGSMLVAIPVFWLIRDKAAAQPAALWLSLVLSVFINYPHFAHSYQLLYAGIGQRILGR
                     NTTRKVSLKYVWAGFVAPALIGAFLLGAYLTGNVRVLGYAANAVFFSVGWHYVKQGYG
                     VITVLSAVRRIYYSDVEKKLLLLNGYTVWIHSWMMMNVALREQTYYGVKYFLLDLPPM
                     ALTIGYAATALTTLALFVAFGRRVARKQPVSWNGIVGYVCSLYLWVVAFYADPIFTLF
                     VPAFHSLQYLLFVWRYQVNKAHAEAAGAIDAPIPGERRTVAIRVARFVLVGMVLGFIG
                     FMALPTLLEASLAPDPAIWGGTVFVFILSAFINLHHYFIDNVIWRRDNEDVRRYLFAP
                     R"
BASE COUNT          539 a          905 c          981 g          504 t
ORIGIN      
        1 gacgcggcga ggcacgtcga gacgcgcccg atcaagggca cgcgcccgcg cggcgtcggt
       61 cccgagcacg atgcagcgct cggccaggcg ctcgccgaga gcgcgaagga tcgggccgag
      121 aacctgatga tcgtggacct gatgcgcaac gacctctcgc gcgtgtgcgc gccgggcacg
      181 gttcgcgtgt cggagctgtt ttcgctggag cactacgcca cggtccatca cctcgtctcg
      241 acggtggtcg gagagctcgc gccgggtgcc gacgcgatcg atctgctgcg cgcgtcgttc
      301 ccgggcgggt cgattacggg ggcgcccaag ctgcgggcga tggaggtgat tgccgagctc
      361 gagccgtcgc ggcggggggt ctactgcgga gcgatcggct actggagcct gagcggcgag
      421 ctcgacacca gcatcgccat ccgcacggcc ctcgtgcagg gcgaccgcat ctacttcagc
      481 gccgggggtg gcatcgtcgc cgattcggat ccggaggagg agtaccgcga gacgctcgac
      541 aaggctcgcg ccatgatcga cacgctcgcc ttgggagcac tgagccgatt gtagatgttg
      601 cgatctttga ttgttgattc gttggtgatt gtcgactggt gattggtgat ttattggtcg
      661 attggaacgt gctgggcggc atccaggccg cgtcctagcg ggctggcgat agagtgaccg
      721 gtagcggcgt tgattcgttg ccgcttgtcg aatgattgcg gaaccgtggc ctcaatcacc
      781 aacaaatctc caatcaacaa tcgacaatca gcaacgaatc gataatcaac aatcaagaat
      841 catcaatgac tcgccctcac gtctacgact tctgaacctc gaagccgtcc acctgcactt
      901 ctgtcacgtg gttcacgcgg aaccccacga cgccatcggt cggcgcgttg agctccgcct
      961 tcggcgtcgt gtgcaccacc atgccgttga cgacgtaggt gaccgtgtca cccgacacgc
     1021 gcacttcgag cgcgttgacc gacctgccgc tggcatcggg cctcgtgacc gcggggctgg
     1081 gcgtacgtcc cttgacatct ctcgtcgctg cgcccgtccg ctgcttgatg aggaacgtgc
     1141 cgtcctgcgc cactgtgaag tacgtgtagc gctggtcggc accgtcgagt ccctgcccgc
     1201 cgaacacgat gccgtagtag ttggtgtggc tgctcggctt caacagcgtg aacgtaccct
     1261 tcgcggtgta gttgccggcg gccgtcgtgg acggattcca gtagacgccg gcgggcccgc
     1321 ccgcgacctg aaggcccttg cctgccgccc tgaacgtgac gcccgacttg tcgtcgggat
     1381 cctgagcgct cgtactcttg tcgagccgca tcttccagcc ggcgggagcc tgcgccgaca
     1441 acgggacggc gagtgacgct atgagagctg cgagaacaac gtgtttgaac atcgatccga
     1501 tctccttggg cctgaccctt ccaggcgaag agtgtttgtg gccggctcgg ccgtatcggc
     1561 gggtgttgcc ggcgtgtgca gggaattatc ccaccggcga cgcgggactc gtcaacgcgg
     1621 cgcgaacagg tagcgccgca cgtcctcgtt gtcccgccgc cagatcacgt tgtcgatgaa
     1681 gtagtgatgg aggttgatga aggcggagag gatgaacacg aagaccgtcc cgccccagat
     1741 ggcgggatcg ggcgccaggc tcgcctcgag aagcgtgggc agcgccatga atccgatgaa
     1801 ccccagcacc atgccgacga ggacgaacct cgcgactctt atggccacgg tcctcctctc
     1861 cccgggtatc ggcgcatcga tggcccccgc ggcttcggcg tgtgccttgt tcacctggta
     1921 gcgccagacg aacagaaggt actggagcga atggaaggcc ggcacgaaca gcgtgaagat
     1981 cgggtcggca tagaacgcga cgacccacag atagagcgag cagacgtagc cgacgatgcc
     2041 attccaggag accggctgct tgcgcgcgac gcggcggccg aacgcgacga acagcgcgag
     2101 cgtcgtgagc gccgtcgcgg cgtagccgat ggtcaacgcc atcggcggca ggtcgagcag
     2161 gaaatacttc acgccgtagt acgtctgctc gcgcaacgcg acgttcatca tcatccacga
     2221 atgaatccag acggtgtagc cgttgaggag cagcagcttc ttctcgacgt cggagtagta
     2281 gatccggcga accgccgaga ggacggtgat gacgccgtaa ccctgcttga cgtagtgcca
     2341 gccgacagag aagaacacgg cgttggcggc gtagccgagc acgcggacgt tgccggtcag
     2401 atacgcaccg aggagaaacg cgccgatcag cgccggcgcg acaaagcccg cccagacgta
     2461 cttcaggctg accttccgtg tggtgttccg cccgaggatc cgctggccga tgcccgcgta
     2521 gagcagctga tacgagtgcg cgaagtgcgg gtagttgatg aagacgctca gcacgaggct
     2581 gagccagagc gccgccggct gcgcggcggc cttgtcacgt atgagccaga aaacgggaat
     2641 cgcgacgagc atcgagccgc cgccggccat caggaagtcc gtaaggggat gaaacaggta
     2701 gccggaatgg cgcggcgccg ccgctgcggc caggcttgcg gccgcctccg caggcgcgac
     2761 atcgggccgc gcggtcgctg cacttcggga catcactagt ggaccgtatc aactaattcg
     2821 gttgtgggca cgttaagcct ccgatcacgg tctggcggcc gcgacaacgg agcggccgct
     2881 actgattcgg gagttacgct ccactagagg tatcggtgtg gcggcgaag
//
LOCUS       DBNGSH010000151         4014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_690255, whole genome shotgun sequence.
ACCESSION   DBNGSH010000151 DBNGSH010000000
VERSION     DBNGSH010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_690255"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC16CY_03535"
     CDS             <1..228
                     /locus_tag="AC16CY_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl-peptidase 3 family protein"
                     /protein_id="HIR3352879.1"
                     /translation="NGTFAVNPERIRQNVTDLTRDIMTLQARGDYAAAKEMMDKLAIV
                     RPQVQIVLDKLKAVPVDIEPRFVTADTLLAR"
     gene            775..1977
                     /locus_tag="AC16CY_03540"
     CDS             775..1977
                     /locus_tag="AC16CY_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3352880.1"
                     /translation="MNGRLVLVLVVLTAAGVLVARELAGQSRNAVQAPYFEVDPLWPQ
                     PLPNHWLLGSTIGVSVDERDHVWIIHRSSSTLDDNEKGLELSPKRGECCAGAPPVLEF
                     DADGKLVGHWGGPGTGYEWPQSNHGITADLERNVWIGGNGASDSHVLKFTRDGKFLMQ
                     AGKQHARKGPNNAQGQPTYVGGSNDPQSFGRVAKIFVDEASNEAYLADGYLNRRVAVI
                     DTETGKIKRHWGAYGNKPDDRNLGTYDPNAKALDQFRNPVHCADLSKDGFVYVCDRVG
                     NRLQVFTKDGKFVKEAFFAKGTLGAGSAWDVAFSRDAQQTYIYLADGLNMKVRVLRRD
                     TLEELTTFGDGGRQPGQFYGVHSIATDSKGNVYTTETYEGKRVQKFVYRGLRAVTRRD
                     QGTVWPAK"
     gene            2076..>4014
                     /locus_tag="AC16CY_03545"
     CDS             2076..>4014
                     /locus_tag="AC16CY_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HIR3352881.1"
                     /translation="MISVVPVLVAAALAAQARPTSSEWRTYGADLASTRYAPLDQINA
                     ANFGTLEVAWRFKTDNLGPRPEFNFQSTPLVVGGMLYTTAGSRRAVVALDAATGEMKW
                     MHALDEGMRGAEAPRLLSGRGLAYWSDGKSSRVIYVTPGYQMIALDARTGQRIPSFGR
                     GGIVDLKLEDDQEMDPVTGDIGLHAAPIIARDVVVIGAAHTEGSRPVSRRNDKGYVRG
                     YDVRTGKRLWIFHTIPMPGEMGADTWEQGSAEYTGNNGVWGQMSADESLGLVYLPTEM
                     PTGDYYGGHRPGANLFSDSLVALDIRTGKRKWHYQFIQHDIWDWDLPCAPILADVTID
                     GRPRKIIAQPTKQGWLYVFDRETGQPIWPIEERPVAPSDVPGEKAHPTQKFVTKPPAY
                     ERQGVSIDDLIDFTPELRAEAVKLVSRFKIGPIFTPPVVSRFDGPLGTLMLPNVTGGA
                     NWQGGSFDPETGIFYIFTNNEVASVGLVPGAERPGEPSDMTYVRGQARNPANPKAPDI
                     PVTVQGLPLVKPPYGRISAIDLNKGDLLWQIAHGETPDNIRNHPRLKGLAIPRTGRQG
                     RIGTLVTRSLLVAGEGGLFTLPDGRRGAMLRAYNKATGQEVGSVYMPAPQTGSPMTYL
                     LNGQQYIVVAISGPGFPGELIA"
BASE COUNT          726 a         1324 c         1363 g          601 t
ORIGIN      
        1 aacggcacgt ttgccgtcaa ccccgagcgc atcaggcaga acgtcaccga cctcacgcgc
       61 gacatcatga cgctgcaggc cagaggggac tacgcggcgg ccaaggagat gatggacaag
      121 ctcgccatcg tccggccgca ggtgcagatc gtcctcgaca agctgaaggc cgtacccgtg
      181 gatatcgaac cgcgctttgt cacggcggat acgctcctgg cgcgttgagg gctcgagcgg
      241 agctcagttg tatgtggaac gtcggcaatc ccgcgagccg acgcggaaaa aacgtgcttc
      301 cggcactccc gtcggcacac gttcatcttt cttgctggtt gccacgtctt cgccgcgcgg
      361 cgagcctcca gcacgtttct acaccatcgt tgatcgcgtc gcctcgaagg gcttgccgcc
      421 attcctcaca caactgagct ccgcactcag gtagccggcc cgtctgatgt gtcctggcgc
      481 gacggcgtgt cgcaagcccg tctcgcgaca gttcgccacc ggcattgcag gcgtggaggt
      541 gcgggggagg agcgagagcg acagaggttc gcgacggcgg agcgccgact caccgtcaga
      601 cacgcgccgg agatgccggt ggccgggcgc tgcgacccgg ggtccccagc gcgcacgcct
      661 gcgcgcgggg gtggaggccc gcagcgcgga aggacacgtc aggcggaccg gctactttga
      721 ccttcgaaca ttgaacttgc ataatccccg cggaggtctc ccgtgcacaa gatcatgaac
      781 ggcaggctgg tcctcgtgct ggtcgtgctc accgcagctg gtgttctcgt ggcccgggag
      841 ctcgcgggcc agagccgcaa cgcggttcag gcgccgtact tcgaagtcga tccgttatgg
      901 ccccagccgc tgccgaatca ctggctgctg ggttccacca tcggcgtctc ggtggacgag
      961 cgcgaccacg tctggatcat ccaccgcagt tcctcgacgc tcgacgacaa cgagaagggg
     1021 ttggagctgt cgccgaagcg cggcgagtgc tgtgcgggag cgccgccggt gctcgagttc
     1081 gacgccgacg gcaagctcgt cgggcactgg ggcggaccgg gcacggggta cgagtggccc
     1141 cagtcgaacc acggcatcac ggccgatctc gagagaaacg tctggattgg cggcaacggc
     1201 gcctccgact cgcacgtgtt gaagttcacc cgcgacggca agttcctgat gcaggcgggc
     1261 aagcagcacg cgcgcaaggg gccgaacaac gcgcaggggc agccgaccta cgtcggcggg
     1321 agcaacgacc ctcagagctt cggccgcgtg gcgaagatct tcgtggacga agccagcaac
     1381 gaggcctatc tggccgacgg ctacttgaac cggcgcgtcg cggtcatcga caccgaaacc
     1441 gggaagatca agcggcactg gggcgcctac gggaacaagc ccgacgaccg gaaccttgga
     1501 acctacgatc ccaacgcgaa ggcgctcgat cagtttcgca acccggtgca ctgcgccgac
     1561 ctctcgaagg acggcttcgt gtacgtgtgc gaccgcgtcg gcaacaggct gcaggtgttc
     1621 accaaggacg gcaagttcgt gaaggaggcg ttcttcgcca agggcacgct cggcgcgggc
     1681 tcggcgtggg acgtcgcttt ctcgcgagac gcgcagcaga cctacatcta cctcgccgac
     1741 ggcctgaaca tgaaggtgcg cgtccttcgc cgcgacacgc tcgaggagct cacgacgttc
     1801 ggcgacggcg ggcgccagcc gggccagttc tacggcgtgc acagcatcgc gacagactcg
     1861 aagggcaacg tctacacgac ggaaacctac gaagggaagc gcgtgcagaa gttcgtctac
     1921 aggggccttc gcgcggtgac gcggcgggat cagggcacgg tgtggccggc gaagtgatgc
     1981 gatctgcccc gaagataaac cacgaaggcc acgaagatca cgaaggtcac gaaaggttac
     2041 cgatattgct tcgtgttctt cgtggcagat tcttcgtgat ctccgtggtt ccggtgctgg
     2101 ttgctgcggc gctcgcggcg caggcccggc ccacttccag cgagtggcgc acctacggcg
     2161 ccgatctcgc gagcacgcgc tacgcgccgc tcgaccagat caacgccgcc aacttcggca
     2221 cgctcgaagt ggcgtggcgc ttcaagaccg acaacctggg cccgaggccc gaattcaact
     2281 tccagtccac gccgctcgtg gtgggcggca tgctgtacac gacggccggg tcccgccgcg
     2341 cggtcgtcgc cctcgatgcg gctaccggcg agatgaagtg gatgcacgcc ctcgacgaag
     2401 gcatgcgcgg cgcggaggcg ccgcgcctgc tgtcgggccg cggactggcg tactggtccg
     2461 acggcaagtc gtcgcgcgtg atctacgtca cgcccgggta ccagatgatc gccctcgatg
     2521 cgcggaccgg ccagcgcatc ccgtcgttcg gacgcggcgg catcgtggac ctgaagctcg
     2581 aagacgacca ggagatggat cccgtgacgg gtgacatcgg gctgcacgcc gctcccatca
     2641 tcgccaggga cgtcgtcgtg atcggggcgg cgcacaccga gggcagccgg cccgtcagcc
     2701 gccgcaacga caagggctac gtgcgcgggt acgacgtgcg cacggggaaa cggctgtgga
     2761 tcttccacac catccccatg ccgggcgaga tgggcgccga cacgtgggag caggggtccg
     2821 ccgagtacac gggcaacaac ggcgtctggg ggcagatgag cgcggacgag tcgctcggtc
     2881 tggtctacct gccgacggag atgccgaccg gcgactatta cggcggacac cggcccggcg
     2941 cgaatctctt cagcgacagc ctcgtcgcgc tcgacatcag gacgggcaag cggaagtggc
     3001 actaccagtt catccagcac gacatctggg actgggatct gccgtgcgcg ccgatcctcg
     3061 ccgacgtcac gatcgacggc cggccgagga agatcatcgc ccagccgacg aaacagggtt
     3121 ggctgtatgt cttcgaccgc gagaccgggc agccgatctg gccgatcgag gaacgccccg
     3181 tggcaccgtc ggacgtgccg ggcgagaagg cgcaccccac gcagaagttc gtcacgaagc
     3241 cgccggccta cgagcggcag ggcgtctcga tcgacgatct gatcgacttc acgccggagc
     3301 tgcgagccga agcggtcaag ctggtgtcgc gcttcaagat cggtccgatc ttcacgcccc
     3361 ctgtcgtcag ccgcttcgac ggcccgctgg ggacgctgat gctgccgaac gtgacgggcg
     3421 gcgccaactg gcagggcgga tccttcgatc ccgagacggg catcttctac atcttcacga
     3481 acaacgaggt ggcctcggtc ggcctggtgc cgggcgcgga gcggccgggg gagccgtcgg
     3541 acatgacgta cgtccgcggc caggcgcgca atccggcgaa cccgaaggcg ccggacatcc
     3601 cagtcacggt gcagggactt ccgctggtca agccgccgta cggaagaatc agcgcgatcg
     3661 atctgaacaa aggcgacctc ctctggcaaa tcgcgcacgg cgagacgccc gacaacatcc
     3721 ggaatcatcc gcggctcaag ggactggcga ttccgcgtac cgggcgccag gggcgcatcg
     3781 gcacgctcgt gacgcgcagc ctcctcgtcg cgggcgaggg cggtctcttc acgctgcccg
     3841 acggccggcg cggcgcgatg ctgcgggcct acaacaaggc gaccggtcag gaagtgggct
     3901 cggtctacat gccggctccg cagaccgggt cgccgatgac ctacctcctc aacggccagc
     3961 agtacatcgt ggtcgccatc agcggccccg ggtttcctgg cgagctgatc gcgt
//
LOCUS       DBNGSH010000152         1532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4086447, whole genome shotgun sequence.
ACCESSION   DBNGSH010000152 DBNGSH010000000
VERSION     DBNGSH010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4086447"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="AC16CY_03550"
     CDS             complement(<1..647)
                     /locus_tag="AC16CY_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein"
                     /protein_id="HIR3352882.1"
                     /translation="MILTLEIMGPREGTPRGEDRKVFKAAGGTIGRLPDNDWVLTDPY
                     VSSRHARIRYANGAFQIEDTSTNGVFINSPDNRLVRGQPYTLQSGDRIFIDPYEIRAS
                     VAAESADEADPFRVDDLFGQPVRSAPMPSAPSDSRPRPTPSPLVTGGDEVDPLRALGF
                     DPGKAPVPSVPRASDLAAGSLLSEHYQAPEPVRSEPPPSQGAGLIPDDYDPLQSGP"
     gene            complement(663..>1532)
                     /locus_tag="AC16CY_03555"
     CDS             complement(663..>1532)
                     /locus_tag="AC16CY_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352883.1"
                     /translation="ILAAEEADRAIDATKVTLTPELAESRTAARGNVANARLRLGVAE
                     KTQNAAAAAEAQGLARNASDALAKVLEGVRKLAGEQLLLAVSTAEQEFSFLDTSFGRL
                     NQLFTEKPQMVKPEMTTQRDALQKDLAAARRRFDSARKTESVQGVQDAVRLASEARMR
                     LDALLSILAPLTLRDRGVVAALEEGARYYLDGQYQKALDALGPPEQLTNVLLQVHVHV
                     FRAASLYALYLSSGQSDQALRDRASDEVRRSREIDPAFRPSRRAFTPRFITFYEAGAA
                     PATSSPTAPAPPQ"
BASE COUNT          229 a          508 c          536 g          259 t
ORIGIN      
        1 gggcccgact gcaagggatc gtaatcgtcg ggtatgagac cggcgccctg agaaggcgga
       61 ggttcggacc gtacaggttc cggcgcctgg tagtgctcgg acaacaacga gccggccgcc
      121 agatccgacg cgcgcggcac gcttggaacg ggcgccttgc ccggatcgaa cccgagcgct
      181 ctcagcggat cgacctcgtc cccgcctgtc acgagcggcg acggtgtcgg gcgcggccgc
      241 gagtcggatg gtgcggacgg catcggcgcg gagcggaccg gctgtccaaa caggtcatcg
      301 accctgaaag gatcggcctc gtccgcggac tccgcggcga ccgacgctcg aatctcgtat
      361 gggtcgatga agatgcgatc gcccgactgc agcgtgtacg gctgtccgcg cacgagccgg
      421 ttgtcgggag agttgatgaa gacgccgttg gtgctcgtgt cctcgatctg gaaggcgcca
      481 ttcgcgtagc ggattctggc gtgccggctc gagacgtagg gatcggtcag gacccagtcg
      541 ttgtcgggca ggcgtccaat cgtgccgccg gcggccttga acaccttgcg gtcctcaccg
      601 cgcggcgtcc cttcccgcgg ccccatgatt tccagcgtga ggatcacggt gcgcccaccc
      661 tcttactgcg gcggggctgg cgcagtgggg ctcgaggtcg ctggcgcagc gccggcctcg
      721 tagaaggtga tgaagcgtgg tgtgaacgcg cgccggcttg gccggaacgc aggatcgatt
      781 tcccggctgc gccgaacttc gtccgacgcc cggtcgcgaa gcgcctgatc cgactgcccc
      841 gacgacagat agagcgcgta cagcgacgcg gcgcggaaca cgtggacgtg cacctgcagc
      901 agcacgttgg tcaactgttc cggcggcccc agcgcgtcga gcgccttctg atactgaccg
      961 tccaggtagt agcgcgcgcc ctcttccagc gcggcgacca cgccgcgatc gcgcagcgtg
     1021 agcggcgcga ggatgctgag gagcgcgtcg agccgcatgc gggcctctga ggcgaggcga
     1081 accgcatcct ggactccctg aacgctttca gtttttcgtg cgctgtcgaa tcggcgccgc
     1141 gccgccgcca ggtccttttg cagcgcatcg cgctgggtgg tcatctcagg cttgaccatc
     1201 tgcggcttct cggtgaacag ctgattgagc cggccgaagg acgtatcgag gaaggagaac
     1261 tcctgctcgg ctgtcgagac cgcgagcaga agctgctcgc cggccagctt gcgcaccccc
     1321 tcgagcacct tggcgagtgc atcggacgcg ttccgggcga ggccctgcgc ttcggctgcc
     1381 gccgcggcgt tctgagtctt ctcggccacg ccgagccgca gccgcgcatt cgccacattc
     1441 ccgcgggctg cggtccggga ctcggccagc tccggtgtca gcgtgacttt cgtggcgtcg
     1501 atcgcacggt cggcctcttc ggctgccagg at
//
LOCUS       DBNGSH010000153         5659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1878596, whole genome shotgun sequence.
ACCESSION   DBNGSH010000153 DBNGSH010000000
VERSION     DBNGSH010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1878596"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..2707
                     /locus_tag="AC16CY_03560"
     CDS             128..2707
                     /locus_tag="AC16CY_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3352884.1"
                     /translation="MGRLLRRLRYWLRQDNHEAALAEELEFHRAMKQQDLEADGLAPE
                     HARFAAHRAMGNVTLAREDSRAQWIWPWLESVGQDTRIAVRALLRQPGFTLLSVVVLG
                     VAIGLNTSVFTVFAGAALRPMTGVTDPDGLAIVSGRVPQVPNLLSGLSIPEFEFLAAR
                     ATTAELAAERRTVVSFDSGGGARSVTTLFVSGNYFDLLGAGLQHGRGILAADDARGAG
                     EAVVVLSHVLWQTQFGGDPSVIGRRVRVAGQPHTVVGVAARDFGGAEGRARVWLPLTS
                     LPALLPNDIGRGDPELCCVEVYARLDEGVTRTQLATELDAVSARFRASAGQDARAIVV
                     GGTQALRGRRGDREGLAVIGVLFLGVTMVLLLACANVGNLLLARAVARRSEIGVRLSL
                     GAGRRRLVRQLLTEGMLLALIAAGAGLAVAAWLPRFLINEVVGEAAPFDIDLDGMVFV
                     YAALLAGLACVASALAPALHATRGDIGASLKGTAVALRFPLRSVLLGAQIAITVVLLA
                     SAGLLLRGVAAARDMDLGYAIEDIDEVMVRLPQPDEAARVQALVPELLTALDAAGFTG
                     VALAAAGVTTDEARVPGAPADRTRPVHRFDVSASYFSVLDIPVVVGRSFTDADRNRGL
                     VVVNEAFARRFWGGDDAVGRAFVANDRALEVIGVARDARLLGLDPAPPIYFQPLTGRR
                     GPLFPVLLVRRQSADIDDLSALITRVEPRAEVISTPMRNRLDFELSALRLAPLAASGL
                     GLFALGLATVGMFGVFAYAVRQRSREIGIRLALGARSRDLIRGVLSTSARAVVYGLVA
                     GVLAAVGASQLLRGSLYGVSPLDPLTYASVALVIAAAAMVASYAPARRALNVNPVSLL
                     REE"
     gene            complement(2727..3344)
                     /locus_tag="AC16CY_03565"
     CDS             complement(2727..3344)
                     /locus_tag="AC16CY_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3352885.1"
                     /translation="MCRMLQACALIVSVAVAIEPRLGAQQPASEVPNLGAGNTLTLPA
                     DFREWVFLSAGLGMTYGPNAPAAGQPQSFTNVYVNPSSYRAFLASGRWPERTMFILEI
                     RGSASEGSINRFGRFQTGLRAVEVNLKDARLPGGWGFFDFARGAGPVAPLPQSASCYS
                     CHRDNTAVEQTFVQFYPTLMEAARRHGTVKASYRETPESPAAGQR"
     gene            3470..4429
                     /locus_tag="AC16CY_03570"
     CDS             3470..4429
                     /locus_tag="AC16CY_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIR3352886.1"
                     /translation="MARLTRRGFVKATGAVAALPALTALSLRAQGQAPDTGNLRVEKD
                     IVFGKGGDKDLTLDVYRPPAGVTSKRLAIVHLFGGGFFTGNKNAGYIINDAKALGARG
                     YTSVSANYRLQTEGLWPAQIHDTKAAIRWTRANAEKIGVDANRIVVAGYSAGGMLALM
                     AAGTNGNKEFEGTGGNAGVSSDVNACIGVYPLASAQIAGGLFGGKATPEQTAAASPTT
                     YISKSFAPTIFIHGTADTTVLPQSSIDFHTKLHALGVPSALTLIQGANHAFDNAALDA
                     VELMAQSIDLFLDRLIVNPTPYQGFGGGGGGRGRGGRGRGGQN"
     gene            complement(4848..>5659)
                     /locus_tag="AC16CY_03575"
     CDS             complement(4848..>5659)
                     /locus_tag="AC16CY_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352887.1"
                     /translation="IVIGATPTQKGVWIGGNLGFCNANRAPAPASLNPGATGGPGAVT
                     PAAPAGAGRAGAAPAGQAGRGGGRGRGAAGPCTPVPYMEWTLAVSDDRRRNELEPHTR
                     CKPSGGPRQWLTPYGAEFLELPEQKVAYIFDIGGPHTYRTIFMDGRAHPKDLAPSFYG
                     HSIGWWEGDTLVVDTVGFNEGFWIDRGQLPHTDKLHLIERYTRTSLTSMRYELTIDDP
                     AAYKAPFTATSNLRWENGTELFEYICQQANQAHELMVGAGTEVDRSTLIIP"
BASE COUNT          832 a         1905 c         1949 g          973 t
ORIGIN      
        1 ggggggcttg acaagccctc tcccatcgtc agacgatggg agacatggcg gagcacgagc
       61 ctcctgatct cctgcagcat ctcaagaccg cctcgcagga cgtcctccag gtgctcgcca
      121 cctaaccatg ggacgcctgc tccgccgcct ccgctactgg ctccgccagg acaaccacga
      181 ggccgccctc gccgaggagc tcgagttcca tcgcgcgatg aagcagcagg atctcgaggc
      241 tgacgggctc gctcctgagc acgcccgctt cgccgcgcac cgcgcgatgg gcaacgtgac
      301 gctggcgcgt gaggactcgc gcgcgcagtg gatctggccg tggctcgaga gcgtcgggca
      361 ggacacccgc atcgctgttc gcgcgctgct cagacagcca ggcttcacgc tgctttcggt
      421 cgtcgtcctc ggtgtcgcca ttgggctcaa taccagtgtg ttcacggtgt ttgccggtgc
      481 ggcactccgg ccgatgacgg gcgtcaccga ccccgacggc ctggccattg tttccggacg
      541 tgtgccccag gtcccgaatc tcctgtctgg cctgtcgata cccgagttcg agtttctcgc
      601 ggcacgggcg accacggccg aactggcggc ggaacgacgg accgttgtct ccttcgacag
      661 cggcggcggc gcgcgctcgg tgacgacgct cttcgtctcc ggcaactact tcgatctgct
      721 cggcgcgggc ctgcagcacg gccgcggcat cctcgcggcc gacgacgccc gtggtgccgg
      781 ggaagctgtc gttgtcctga gccacgtcct gtggcagacg cagttcggcg gcgatccgtc
      841 cgtcatcggc cgacgcgtcc gcgttgcggg tcagcctcac accgtcgtcg gtgtcgccgc
      901 tcgcgacttc ggcggtgcag aggggcgcgc acgcgtctgg ctgccgctca cctcgctccc
      961 ggccctgctc cccaacgaca ttggacgggg ggatcctgag ttgtgctgcg tcgaggtcta
     1021 cgcgcgtctc gacgagggcg tgacacgcac gcagctcgcg acggagctcg acgcggtgag
     1081 cgcccgcttc cgcgcgtcgg ccggccagga tgcgagagcg attgtggtcg ggggaactca
     1141 ggcgctgagg ggtcgccgtg gcgaccgtga ggggcttgcc gtgatcggcg tgctgttcct
     1201 cggagtcacg atggtgctgc tactcgcgtg cgcaaacgtc ggcaatctgc tgctggctcg
     1261 cgccgtcgcg cggcgcagcg agatcggcgt gcgcctctcg ctcggggcag gacgaagacg
     1321 cctcgtccgt cagctcctga ccgaaggcat gcttctggcg ctgatcgcgg ccggcgccgg
     1381 cctcgccgtc gcggcatggc tgccgcgctt cctgattaac gaagtcgtcg gagaggctgc
     1441 accattcgac atcgatctcg atggcatggt gttcgtctac gcggccctgc tcgcgggcct
     1501 ggcctgcgtg gcctctgcgc tcgcgccggc gcttcacgcc acccgcggcg acatcggcgc
     1561 gtcgctgaaa ggcacggcgg tcgcattgcg ctttccgctc cgcagcgtgc tgctcggcgc
     1621 gcagatcgcc atcacggtgg tgctgctggc gagcgccggc ctgctgctgc gtggcgtggc
     1681 agccgcgcgc gacatggatc tcggatacgc catcgaggac atcgacgagg tgatggtccg
     1741 gctgccgcag ccggatgagg ccgcgcgcgt gcaggccctc gtgcccgagc tgttgacggc
     1801 gctggacgcg gcgggcttca cgggggtggc actggccgcg gctggagtga cgaccgatga
     1861 agcccgtgtc cctggtgcgc ccgccgaccg gacacgtccc gtccaccgct tcgatgtctc
     1921 ggcgtcgtac ttcagcgtgc tcgacatccc ggtcgtggtc ggcaggagct ttacggacgc
     1981 cgatcgcaat cggggcctcg tcgtcgtcaa cgaagcgttc gcgcgccggt tctggggcgg
     2041 cgacgacgcc gtcggccgcg cgttcgtcgc caacgaccgt gcgctggaag tcatcggtgt
     2101 tgcccgcgac gcacgactgc tcggcctgga ccccgcgccg ccgatctact ttcagccgct
     2161 gaccggacgg cgaggccctc tcttcccggt gcttcttgtc cgtcgccagt ccgcggacat
     2221 cgacgacctc tcggcgctga tcacgcgggt cgagccccgc gcggaggtga tctcgacccc
     2281 aatgcggaac cggctcgact tcgaactgtc cgccctccgg ctggcgccgc tggcggcgtc
     2341 cgggctggga ctgttcgcgc ttggcctggc gacggtcggc atgttcggcg tgttcgcgta
     2401 tgccgtacgg cagagaagcc gcgagatcgg gatccgcctt gcgctcggcg cgcgatcgag
     2461 agatctcatc cgcggcgtgc tctcgacgag tgcccgtgcg gtggtttatg ggcttgtcgc
     2521 cggcgtcctt gccgccgttg gcgcgtcgca actgcttcga gggtcgctct acggcgtcag
     2581 cccgctggat ccgctcacct acgcgagcgt tgcgctggtg attgccgccg cggcgatggt
     2641 tgccagctat gcccccgctc gtcgcgcgct caacgtcaat cccgtttcct tgctgcgcga
     2701 ggaatagcga gagggggcca ccgggtttat cgctggccgg ctgccggcga ctccggtgtc
     2761 tcgcgatagc tcgctttcac cgtgccgtgc cgccgagccg cctccatcag cgtcgggtag
     2821 aactgcacga aggtctgctc gacggcggtg ttgtcgcggt gacacgaata gcagctcgca
     2881 ctctgcggca gcggcgccac ggggccggcg ccacgggcga agtcgaagaa tccccacccg
     2941 ccggggaggc gcgcgtcctt cagattgact tcgaccgcgc ggagcccggt ctgaaatcgc
     3001 ccgaaccggt tgatggagcc ttccgaggcc gagcccctga tctcgaggat gaacatcgtg
     3061 cgctcgggcc acctgccgct cgccaggaag gcgcgatacg aggaagggtt cacgtagacg
     3121 ttcgtaaacg actgcggctg cccggccgcc ggcgcattgg gaccgtatgt catgccgagg
     3181 cccgcgctca ggaagaccca ctcgcgaaag tcagccggga gcgtcagggt gtttcctgcg
     3241 ccaaggttgg gtacctcgct ggccggctgc tgtgcgccga gcctcggctc gatggcgacg
     3301 gcaacggaga cgatgagcgc acaggcctga agcattctgc acatgcctga atcgtaaccc
     3361 cgaaagcgcg ccggatcttt gccagatcat atcggggaaa ttccggtagg ataccggaga
     3421 ctcctgcatt tcggtttcgc acgtcatcca acaaggggga acgacgatca tggcgcgact
     3481 caccaggcgg ggattcgtca aggcgacggg cgcagtggcc gcgctgccgg cactcaccgc
     3541 gctctcgctg cgggctcagg ggcaggcacc ggacacgggg aacctgcgcg tcgagaagga
     3601 catcgtgttc ggaaagggtg gagacaagga cctcacgctc gatgtctacc ggcctcccgc
     3661 aggcgtgacg tcgaagcgtc tggcgatcgt gcacctcttc gggggcggct tcttcaccgg
     3721 caacaagaac gccggctaca tcatcaacga cgccaaggcg ctcggcgccc ggggctacac
     3781 gagcgtgtcg gcgaactacc ggctccagac cgaggggctg tggccggcgc agattcacga
     3841 cacgaaagcc gcgatccgat ggacgcgcgc gaacgccgag aaaatcggcg tggatgccaa
     3901 ccggatcgtc gtcgccggct actcggccgg cgggatgctg gcgctcatgg ccgccggcac
     3961 gaacggaaac aaggagttcg agggcactgg cggcaacgct ggcgtgagct cggacgtcaa
     4021 cgcctgcatc ggcgtgtacc cgctggcaag cgcgcagatt gcgggggggc ttttcggggg
     4081 gaaggcgacg cccgagcaaa ccgcggccgc gtcgcccacg acgtatatct ccaagagttt
     4141 cgcgccgacg atcttcattc acggtaccgc cgacaccacg gtgctgccgc agtcgagcat
     4201 cgacttccac acgaagctcc acgcgctcgg cgtgccgtcg gcgctgacgc tcatccaggg
     4261 cgccaaccac gccttcgaca acgccgcgct cgacgcggtc gagttgatgg cccagtcgat
     4321 cgacctgttc ctcgaccggc tgatcgtcaa ccccacaccg taccaggggt tcggcggcgg
     4381 gggcggcggt cgcgggcgtg gtggacgggg ccggggcggc cagaactagc gatctggttc
     4441 ttggtccttt gttcttggtc ctctggtccg tccgtggtcc tgtggtccgt cctgtcgtcc
     4501 caggtccaat ggtccgctga ggaccatgaa cgaaggacca gggacggacc aagcaccaag
     4561 gactaagggc caagcactaa ttagtcgatc gacctgttcc tcgaccggct gatcgtcaac
     4621 ccgacaccgt accaggggtt cggcggcggg ggcggcggcc gcgggcgcgg aggacgcggc
     4681 cggggcggcc agaactagcg tctggttctt ggccctttgt tcttggtcct ctggtcctcc
     4741 gtggtcctgt ggtccgtcct gtcgtcccag gtccaatggt ccgctgagga ccttgaacga
     4801 aggaccaggg acggaccaag caccaaggac taagggccaa gcactaacta ggggatgatc
     4861 agggtactgc ggtcaacctc ggtgccggcc ccgaccatca gttcgtgcgc ctggttcgcc
     4921 tgctgacaga tgtattcgaa gagctcggtg ccgttctccc atcgcaggtt cgacgtcgcg
     4981 gtaaacggcg ccttgtaggc ggcggggtcg tcgatggtca gctcgtatcg catcgacgtc
     5041 agcgacgtgc gcgtgtagcg ctcgatcaga tgcagcttgt cggtgtgcgg cagctggccg
     5101 cgatcgatcc agaagccctc gttgaagccg accgtgtcca cgaccagcgt gtccccctcc
     5161 caccagccga tcgagtggcc gtagaagctg ggcgccagat ccttcggatg ggcacggccg
     5221 tccatgaata tcgtgcggta ggtgtgcggg ccgccgatgt cgaagatgta ggccaccttc
     5281 tgctccggca gctcgaggaa ctcggcgccg tacggcgtca gccactggcg gggcccgccg
     5341 gacggtttgc agcgcgtgtg cggctcgagc tcgtttcgac ggcgatcgtc ggagaccgcc
     5401 agggtccatt ccatgtaggg gaccggcgtg cagggcccgg ccgcgccgcg gcctcgccct
     5461 ccgccacgac cggcctggcc cgctggtgca gcgcccgcac gtcccgcgcc cgctggtgcc
     5521 gcgggagtga cagcgcccgg gcccccggtg gcgcctggat tgagagacgc cggcgctggc
     5581 gccctgttgg cgttgcagaa gccgagattg ccgccgatcc acacaccctt ctgcgtcggc
     5641 gtggcgccga tgacgatgc
//
LOCUS       DBNGSH010000154         6315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1539019, whole genome shotgun sequence.
ACCESSION   DBNGSH010000154 DBNGSH010000000
VERSION     DBNGSH010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1539019"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2408)
                     /locus_tag="AC16CY_03580"
     CDS             complement(<1..2408)
                     /locus_tag="AC16CY_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR3352888.1"
                     /translation="MPATETLSPVEPLTTPYDRERLEDVGFMTSMTLVLLGNYAQTGH
                     FGGPLAYTPYNVATHLAGPDLGGLRFDYRRPKHPYCDKFMLAGGHCIPTCYALWMVMG
                     QALARRYESTGDRRYYADPHVAILPVDALGFRRGAGALKTILQDHGLADHPLFAQAKL
                     RGIRALAGHAESTDVTNDVNGGPSGIGVATAAGKAAFWDVAGAAVDAPKVIAFEGEFA
                     MTEGHAQELKTQALALKVGKRLRVMISDNNAGIDDSLIGGVIDRKYVGYRLIDQWTSY
                     GWNVFVVRDGNDYDQVVAALKTMERWDPADRRPMALIGKTTKGYWPAAVEGRIPDFGD
                     QVVGYPSHPYAMKMNSDYFVALARTFERRYGVEFEGIRLGPVKDPRERLIQFKTNVDV
                     VMSLLDRNGLGDWLADRLVEIGESLPDSIPLRLPLGKDPFQDERLRVANLPVEPQKVT
                     VANPVSGATKDVQITLFRKPGEVAGTRRGVSEIVKWMNYVTGNRFLTLAADLSESINL
                     EHGSLWGHYDPESNPAGTRVKAAIQEAGNVSSAIGLVSQNASLDPDAFAGVWAISGTY
                     GAFTPLMYTPARVWSQQNQDSPFRNGVLHILVGHSGPETAADGRTHFGIFAPQVWKLF
                     PRGQTIHLNFWDYNDVAPAYFAAAEIAAREKKVGIITLEVARPDFPVADRATFADTDL
                     RAAAKGFYVIREFGPGPRHGYVVTQGSSSTVNLVSVLPKLEAAGVNVKVVAAISEELF
                     DRQPASYRASVLPPEARYDLMVVSTGTRRVWPLRDAGPLTDEYSMTSDWDHQWLTSGL
                     EPDV"
     gene            complement(2509..3390)
                     /locus_tag="AC16CY_03585"
     CDS             complement(2509..3390)
                     /locus_tag="AC16CY_03585"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3352889.1"
                     /translation="MKVGFIGLGRMGQGMARRILDAGHDLTVFDLVAAQTTSLGASGA
                     RVATSIADLCAGREVVVTMLVEDRAVDDVALGPGGLCASLARGAVHLVMGTHGVAVIR
                     RLEARHRDAGQTLVAAPVLGRPDLAASGQLGIVAGGPEEAVRTVEPLLRAMGRKTFPA
                     GVKPESATAIKLANNAVLGCAMVAMAEGFSLVRKYGVAPQVFQDVMTEGLFSAPAYKV
                     YGQKMVDESYDQVGSPIHVGLKDANLIAAAADLVHVPMPSHNVYHDRLRGAVAHGDGD
                     RDQAVLAREQARAAGLE"
     gene            complement(3479..>6315)
                     /locus_tag="AC16CY_03590"
     CDS             complement(3479..>6315)
                     /locus_tag="AC16CY_03590"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3352890.1"
                     /translation="IINTGGGAGIMGMTGGYAPDVANSQEVNIQLSGALGESETGGAA
                     INIVPRTGGNRFSGNWYTTYTRQSWFDKNNDVHTEVPITTSLIRDYTISGAFGGPIKR
                     DHLWFFSAGQNWQKDAWNSQNLHIWDNKNRGMWGMNYQPDRTTDPLTLMNKTASINVR
                     VTYQATQKDKINFFWDEGFTCQDPCDGAVAAWAPRDGNWSGQVHPARLRQVSWTNPMT
                     NKILLEAGLAVNTQLYDFSYMRYMPGIMDIPRVQEFGAHVGMDEVSPVVNSTDFTFLG
                     VQSANLNNGPASIAEYRNLDDFRPRASLSYVTGTHNAKFGYDGGYFSQNRHNTANDLR
                     LNYRYDTPNANCLTTAPSATNPYPCGNMSRYFPGDLMNTNRRPVPTQVTMNTGIADIG
                     NKVSYSAFYVQDQWTLNRLTLNGAIRYDHATSSYLETCIGPDLRIPVQVGGAYAGQSQ
                     YCTPPSDGVNYNNITPRWGVVWDVFGNGKTSVKYNMGKYLAGAGVAGIYANANPALRT
                     VNEYTRTWTDGDGDRIVDCDLLNFNAQNNVATGGDNCGGPTSVFGQDATRYGRDPNSL
                     DEAGIPIGLATTQCGRKESGIPANVQAYCNAYGESLIEGWDRRRSEWQLGLGIQHEIL
                     PRLSAEFTYNRRDYANLQVSDQLGNGCDRYNGPDLQTCLDNALNYSAPNHDFFYFTVP
                     VDDRLPDGGGYTVRGNINPKIGFSTAGLSTAQTFMDELEYTSHFFDTNFVWRGPKGIR
                     LNGGTSTGRAFRNTCYTELDAPNVKARDGNNPACNPYQRWDTNVRGTATYTIPYIDVL
                     ASSVFQYRPGDNRAANITITKDQVVWEPSSASRATAACPAGPTAGQVGCFVPQGFTTT
                     ATTTTINALDPGDLYTEGYFIVDLKFAKNFRFANKRLNVGVDVYNLLNNDAIRTYQDT
                     YPATGAGVAYGTPLTLLSPRFVRAQVQFDF"
BASE COUNT         1001 a         2111 c         2059 g         1144 t
ORIGIN      
        1 atgacgtccg gctcgaggcc gctcgtcagc cactggtggt cccagtccga cgtcatcgag
       61 tactcgtcgg tgagcgggcc ggcgtcgcga agcggccaca cgcgcctcgt cccggtggac
      121 accaccatga gatcgtagcg ggcctctggc ggcagcaccg acgcgcggta cgacgccggc
      181 tggcgatcga acagttcctc gctgatcgcg gccacgacct tgacgttcac gccggccgcc
      241 tcgagcttcg gcagcacgct caccagattc acggtggagc tcgacccctg agtcactaca
      301 tagccgtgac gtggccccgg cccgaactcc cggatcacgt agaagccctt cgccgccgcg
      361 cgcaggtccg tatcggcaaa cgtagcgcga tcggcaaccg gaaagtcggg acgggcgacc
      421 tcgagcgtga tgatccccac tttcttctcg cgcgccgcga tttccgccgc ggcgaagtag
      481 gccggggcga cgtcgttgta gtcccagaag ttcaggtgaa tcgtctggcc gcgcgggaac
      541 agcttccaca cctgaggcgc gaagataccg aaatgcgtgc ggccgtcggc cgcggtttcc
      601 gggcccgagt gccccaccag aatgtggagg acgccgtttc gaaacgggct gtcctgattc
      661 tgctggctcc acacgcgcgc gggtgtgtac atcaacggcg tgaacgcacc gtaggtgccg
      721 ctgatcgccc acacgccggc aaacgcgtcg ggatcgagcg aggcgttctg gctcaccagg
      781 ccaatcgcgc tcgagacgtt gcccgcctcc tgaatggccg ccttgacgcg cgtgcccgcc
      841 gggttggact cggggtcgta gtggccccag aggcttccgt gctcgaggtt gatcgactcg
      901 gagagatccg ccgcgagcgt caggaaccgg ttgccggtca cgtagttcat ccacttgacg
      961 atctcggaga cgccgcggcg cgtgccagcc acctccccgg gcttgcggaa gagcgtaatc
     1021 tgcacgtcct tggtggcgcc cgacaccgga ttcgccaccg tgaccttctg gggctcgacc
     1081 ggcaggttcg ccacccggag ccgctcgtcc tgaaacggat ccttgccgag cggcagccgc
     1141 agcgggattg aatcgggaag cgactcgccg atctcgacga gccgatcggc cagccagtcc
     1201 cccaggccgt tgcgatcgag cagcgacatc acgacgtcga cgttggtctt gaactggatc
     1261 agtcgctcgc gcggatcttt caccgggccc aggcgaatcc cctcgaactc gaccccgtag
     1321 cgccgctcga aggttcgcgc gagcgccacg aagtagtcgg agttcatctt catggcgtag
     1381 ggatgactcg ggtagccgac cacttgatcg ccgaaatccg ggatccgccc ctcgacggcc
     1441 gccggccagt accctttggt cgtcttgccg atgagcgcca tcggccgccg gtccgccggg
     1501 tcccaacgct ccatcgtctt cagcgccgcg acgacctggt cgtagtcgtt gccgtcgcgg
     1561 acgacgaaga cgttccagcc gtacgaggtc cactgatcga tcagccggta gccgacgtac
     1621 ttgcgatcga tcacgccgcc gatcagcgaa tcgtcgatgc cggcgttgtt gtcggagatc
     1681 atcacgcgca ggcgcttgcc gaccttgagc gccagcgcct gcgtcttgag ctcctgcgcg
     1741 tgcccttcgg tcatcgcgaa ctcgccttcg aaagcgatca ccttgggagc atcgaccgcg
     1801 gcgccggcca cgtcccagaa ggcggccttg ccggcggcgg tggcaacgcc gatacccgac
     1861 ggacctccgt tgacgtcgtt ggtgacgtcg gtgctctcgg cgtgcccggc gagcgcgcgg
     1921 atgcctcgca acttcgcctg cgcgaaaagc gggtgatcgg cgagcccatg gtcctgcagg
     1981 atcgtcttca gggcgcctgc gccgcgcctg aagccgagcg cgtcgacggg caggatcgcc
     2041 acgtgcggat cggcgtagta gcggcggtcg ccggtggatt cgtacctgcg cgcgagcgcc
     2101 tggcccatca ccatccagag cgcgtagcac gtcggaatac agtggccgcc cgccagcatg
     2161 aacttgtcgc agtaaggatg tttgggccgg cggtagtcga accggagccc gcccagatcc
     2221 ggccccgcga gatgcgtcgc gacgttgtac ggcgtgtagg ccagcggccc gccgaagtgt
     2281 cctgtctgag cgtagttgcc cagcaacacc agggtcatgc tcgtcatgaa cccgacgtct
     2341 tccagacgct cgcgatcgta gggtgtcgtc agcggttcaa ccggggacag cgtctcggtg
     2401 gctggcactc tcgaacctcc agattgcgga ggctgaagcc cccgctctac gtcccgcaac
     2461 cgtagcgcgc aggcttcagc ctgcgcggtg aatagtgtat tcgattgcct actccaagcc
     2521 tgccgcgcgc gcctgttcgc gcgcgagcac cgcctgatcg cggtccccgt cgccgtgcgc
     2581 gaccgcgccg cgcaggcgat cgtgatagac gttgtggctc ggcatcggca cgtgcacgag
     2641 atcggcggcg gcggcaatca ggttcgcgtc cttcagcccc acgtgaatgg gcgagcccac
     2701 ctgatcgtag ctttcgtcga ccatcttctg gccgtacacc ttgtaggcgg gcgcggaaaa
     2761 caggccctcg gtcatcacgt cctggaagac ctgcggcgcc acgccgtact tgcgcacgag
     2821 ggagaagccc tcggccatgg ccaccatcgc gcaaccgagg acggcgttgt tggcgagctt
     2881 gatcgcggtg gccgactcgg gcttcacgcc ggcagggaac gtcttgcgcc ccatggcccg
     2941 caggagcggc tccactgtgc ggacggcctc ttccggcccg ccggcgacga tacccagctg
     3001 gccggaggct gccagatccg gccggcccag aacgggagcg gcaaccagtg tctgcccggc
     3061 gtcgcggtgc cgggcctcga gccgccggat gacggcgacg ccgtgcgtgc ccatgacgag
     3121 atgaacggcg ccgcgcgcga gcgacgcgca gaggccgccc ggtccgagcg cgacgtcgtc
     3181 cacggcccga tcctcgacga gcatcgtgac gacgacctca cgtcccgcgc agaggtcggc
     3241 gattgacgtg gccacgcggg cgccggaggc ccccaaactc gttgtctgcg ccgccaccag
     3301 atcgaacacc gtcaggtcgt gtccggcatc gaggatgcgc cgcgccatcc cctgccccat
     3361 tctgccgagg ccgatgaatc cgactttcat ggtgatccct caaagtcccc gtgagcgtac
     3421 acaaaaacgc ccgcgcattc gaagaatgcg cgggcggcag taggtgaagc ggtcgaactc
     3481 agaagtcgaa ctgcacctgc gcgcgcacga agcgtgggct cagcagcgtg agcggcgtgc
     3541 catacgccac accggccccg gttgccgggt aggtgtcctg ataggtgcgg atcgcgtcgt
     3601 tgttgagcag gttgtagacg tccacgccga cattgaggcg cttgttcgcg aaccggaagt
     3661 tcttggcgaa cttcaggtca acgataaagt agccttcggt atagagatcc cccggatcga
     3721 gggcgttgat ggtggtcgtc gtcgccgtgg tggtgaaccc ctgcggcacg aagcacccga
     3781 cctgaccggc cgtggggccg gcagggcatg ccgccgtcgc ccgagaggcg ctgcttggct
     3841 cccacacgac ctgatccttg gtgatcgtga tgttggcggc ccggttgtca cctggccggt
     3901 actggaacac cgagctcgcc agcacatcga tgtacggaat cgtgtacgtc gcggtgccgc
     3961 gcacgttggt gtcccagcgc tgatagggat tgcaggccgg gttgttgccg tcgcgcgcct
     4021 tgacgttggg cgcgtccagt tccgtgtagc acgtgttgcg gaacgcgcga ccggtgctcg
     4081 tcccgccgtt gagccggatc cccttcggcc cgcgccacac gaagttggtg tcgaagaagt
     4141 gcgaggtgta ctcgagctcg tccatgaacg tctgcgccgt cgagaggccg gcggtcgaga
     4201 agccgatctt cgggttgatg ttcccgcgca ccgtgtagcc gcccccatcc gggagacggt
     4261 cgtcaaccgg cacggtgaaa tagaagaagt cgtggttggg ggccgagtag ttcagcgcgt
     4321 tgtccaggca cgtctgcagg tcgggcccgt tgtaccgatc gcagccgttg ccgagctgat
     4381 cgctgacttg caggttcgcg tagtcccggc ggttgtaggt gaactccgcc gacagccgcg
     4441 gcaggatctc gtgctgaatg ccgagcccca gctgccactc ggagcgccgc cggtcccagc
     4501 cctcgatcag cgattccccg tacgcgttac agtaggcctg cacgttcgcc gggatgccgg
     4561 actctttgcg tccgcactgg gtggtcgcca gaccgatcgg aatccccgcc tcgtccaggc
     4621 tgttcgggtc gcgcccgtag cgcgtcgcat cctgcccgaa caccgacgtc ggaccgccgc
     4681 agttgtcgcc gcccgtggcc acattgttct gcgcgttgaa gttcagcagg tcacagtcca
     4741 cgatgcggtc gccgtccccg tcagtccagg tccgcgtgta ctcgttgacc gtccgcaggg
     4801 ccgggttggc gttggcatag atgccggcaa ccccggcgcc cgccagatac ttgcccatgt
     4861 tgtacttgac cgacgtcttg ccattgccga acacgtccca cacgacaccc caccgcggcg
     4921 tgatgttgtt gtagttgacg ccgtccgacg gcggcgtgca gtactggctc tggccggcat
     4981 acgcgccgcc gacctgcacg gggatccgga ggtcggggcc aatacacgtc tcgagatagc
     5041 tgctcgtcgc gtggtcgtag cggatggcgc cgttcaacgt gagccgattc agcgtccact
     5101 gatcctgcac gtagaaggcg ctgtagctca ccttgttgcc gatgtcggca atgccggtat
     5161 tcatcgtgac ctgcgtgggc acgggccggc gattcgtgtt catcagatcg cccgggaaat
     5221 accggctcat gttgccgcac gggtacgggt tcgtcgccga gggcgccgtc gtgaggcagt
     5281 tggcgttcgg cgtgtcgtag cggtagttca ggcgcaggtc gttggcggta ttgtgccggt
     5341 tctgtgagaa gtacccgccg tcgtagccga acttcgcgtt gtgcgtgccc gtgacgtagg
     5401 acagcgaggc ccgcggccgg aagtcatcga ggttccggta ttccgcgatg ctggccggac
     5461 cgttattgag gttggcggac tgcacgccta agaacgtgaa atcggtcgag ttcacgaccg
     5521 gcgacacttc gtccatgccg acgtgcgcac caaactcctg gacgcgcgga atatccatga
     5581 tccccggcat gtatcgcatg tacgagaagt cgtacagctg cgtgttcacg gccaggcccg
     5641 cttcgagcag gatcttgttg gtcatcgggt tggtccacga cacctgccgc agccgcgccg
     5701 gatgcacctg acccgaccag ttgccgtccc gcggcgccca cgccgccacc gcgccgtcgc
     5761 acgggtcctg acacgtgaag ccctcgtccc agaagaagtt gatcttgtcc ttctgcgtcg
     5821 cctgatacgt gacgcgcacg ttgatgctgg ccgtcttgtt catcaacgtc agcggatccg
     5881 tcgtccgatc cggctggtag ttcatgcccc acatcccgcg gttcttgttg tcccagatgt
     5941 gcaggttctg gctgttccac gcgtccttct gccagttttg cccggccgag aagaaccaca
     6001 ggtggtcgcg cttgatcggc ccgccaaacg cccccgagat cgtgtagtcc cggatgagcg
     6061 atgtagtgat gggcacctcg gtgtgcacgt cgttgttctt gtcgaaccac gactgccgcg
     6121 tgtacgtcgt gtaccagttc ccgctgaacc ggttgccgcc cgtccgcggc acgatgttaa
     6181 tcgctgcccc gccggtctcc gattccccga gcgcgcccga gagctggatg ttcacttcct
     6241 gcgagttggc cacgtccggc gcgtagccgc ccgtcatccc catgatcccc gcgccgccgc
     6301 ccgtgttgat gatcg
//
LOCUS       DBNGSH010000155         1968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2388043, whole genome shotgun sequence.
ACCESSION   DBNGSH010000155 DBNGSH010000000
VERSION     DBNGSH010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2388043"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="AC16CY_03595"
     CDS             <1..201
                     /locus_tag="AC16CY_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HIR3352891.1"
                     /translation="KARGLTSPYLRPFVVARINPIRFSKAASFDFDEVMEKMTASAGK
                     FNIDRVKPEEVTKAAGPPADDD"
     gene            complement(245..1438)
                     /locus_tag="AC16CY_03600"
     CDS             complement(245..1438)
                     /locus_tag="AC16CY_03600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3352892.1"
                     /translation="MPYQFGPFHLDVRERRLSRLGEVIPLRLKVFDTLLVLVENAGRL
                     VTKQELLDTVWPETTVEENNLNHNVSVLRKALGEPATGQQYIETVPRVGYRFAAPVDA
                     TGAPPRPSTSSRSAAAKARQDIRYCTTRDGVRLAYASIGSGPPLVKASNWLTHLDFEW
                     GSPIWRHWHAELSRHHRLVRYDERGNGMSQRDVPDVSFDTWVRDLETVVDAAGLDRFS
                     MLGISRGGPIAIAYAVKHPERLTQLVLYGAFAVGLNPIGTPEQLEARKALTSLIRLGW
                     GLNNPSFCKTFTCRFIPEATPEHEQWLDQLQRVSTSPENAARLMERDDNIDVRPLLSQ
                     VKTPTLVIHCDRDHAVLPEEGRLLAAGIPGARYVSLPSANHLMLEEEPAWSLFLDELG
                     HFLNW"
     gene            1562..>1968
                     /locus_tag="AC16CY_03605"
     CDS             1562..>1968
                     /locus_tag="AC16CY_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HIR3352893.1"
                     /translation="MPQLLPDQSFYPSPTMAMQAPAETLAYVALLNPDPTASDAVAVL
                     DVDPSSPGYGAQIARVDMPHPGDELHHFGWNACSSCLCPNAPHAHMQRRYLVVPGMRS
                     SRIHILDTQPDPRHPTLAKVIEAEEVIRKTGYSR"
BASE COUNT          351 a          642 c          642 g          333 t
ORIGIN      
        1 aaggcgcgcg gcctgaccag tccgtacctc aggccgttcg tcgtggcgcg gatcaatccg
       61 atccgcttct ccaaggccgc aagtttcgac ttcgacgagg tgatggagaa gatgacggcc
      121 tcggcgggga agttcaacat cgatcgcgtg aagccggaag aggtgacgaa ggctgcgggg
      181 ccaccggcgg acgacgactg aaagtggagt cgggctgcga tccgagtgcc cctgatcgtc
      241 attctcacca gttcagaaag tgtccgagtt cgtcgaggaa cagagaccac gccggctcct
      301 cctcgagcat caggtgattc gcactcggga gcgagacgta acgcgcacca ggaatcccgg
      361 ccgccagcag gcgaccctcc tctggcagca ccgcgtgatc gcgatcgcag tggatgacga
      421 gcgtcggcgt cttcacctgc gacaacagcg gtcgaacgtc gatgttgtcg tcgcgctcca
      481 tcagccgcgc cgcgttttcg ggcgacgtcg acacgcgctg gagctgatcc agccactgct
      541 cgtgttccgg cgtcgcctcg gggatgaagc ggcaggtgaa cgtcttgcag aacgatggat
      601 tgttgaggcc ccacccgagg cggatcaggc tcgtgagcgc tttccgcgcc tcgagctgct
      661 cgggcgtccc gatgggattc agtcccactg cgaaggcgcc gtagagcacc agttgcgtga
      721 ggcgctcggg atgcttcacc gcgtatgcga tcgcgatcgg acctccacgg gagatcccga
      781 gcatggaaaa ccgatcgagt cccgccgcgt cgaccaccgt ttcgagatcc cggacccagg
      841 tgtcgaagct cacatcgggt acatcgcgct gcgacatgcc gttgccgcgc tcgtcgtagc
      901 gaaccagccg atggtggcgt gacaactcgg cgtgccaatg ccgccagatc gggctgcccc
      961 attcaaagtc cagatgcgtg agccagttcg acgccttcac gagcggtggt ccgctgccga
     1021 tgctggcgta ggcgaggcgg acgccgtcgc gggtggtgca gtaccggatg tcctgccgcg
     1081 ccttggccgc tgccgaccgt gacgatgtgg aaggccgggg cggcgcgccg gtggcgtcga
     1141 ccggcgccgc gaagcgatag ccgacgcgcg gcaccgtctc gatgtactgc tggccggttg
     1201 ccggttcgcc gagcgccttc cgcagcaccg agacgttgtg gttgaggttg ttctcctcga
     1261 cggttgtctc gggccacacc gtatcgagca gctcctgctt ggtgaccagg cgcccggcgt
     1321 tctcgaccag caccagcagc gtgtcgaaca ccttcaggcg gagcgggatc acctcgccga
     1381 ggcgagacaa ccggcgctcg cgaacgtcga gatgaaaggg cccgaactga tacggcattg
     1441 cgtacgtagc gtccggcttc agccggacct tcagaaaagt ttcagaaata cgccagcgtt
     1501 cccaaaggac gccgccaacc ccgcgatcgt agcctctcgt ggtcacgagg agggcacgac
     1561 aatgccacag ctgcttccag accagagctt ttacccgtcg ccgacgatgg ccatgcaggc
     1621 cccggctgag acgttagcgt atgtcgccct gctcaatcca gacccgaccg ccagcgacgc
     1681 ggtggctgtg ctggatgtcg atccgtcttc gccgggctac ggcgcgcaga tcgcgcgcgt
     1741 cgacatgcca caccctggcg atgagctgca ccacttcgga tggaacgcgt gcagctcgtg
     1801 cctgtgcccg aacgcgccgc atgcgcacat gcagcggcgc tatctcgtgg tgcccggcat
     1861 gcgatcgtcg cgcatccaca ttctcgacac tcagcctgac ccgcgtcacc cgacgctcgc
     1921 gaaggtgatc gaggctgagg aggtcatccg gaagaccggc tacagccg
//
LOCUS       DBNGSH010000156         3276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3576747, whole genome shotgun sequence.
ACCESSION   DBNGSH010000156 DBNGSH010000000
VERSION     DBNGSH010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3576747"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..919
                     /locus_tag="AC16CY_03610"
     CDS             227..919
                     /locus_tag="AC16CY_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352894.1"
                     /translation="MRRFPLFFVTAAAVVALATSGAAQGSGSGKGPTKAPSSAHVPKG
                     GASGSGAGKTTKTTGGSASSTGKTAKSTGASHAGSKKSTTSTDSTTTTTTTTGTGTTP
                     TTNALATKISGNPDQLARVSAMLPAGVTLEDATKGFRNQGQFIAALNASRNRPGIDFL
                     ALQEAMTVDGLSLGQAVKKVQSAPAPTAPTTGGSTTGTGTSTGTDSGGTSTTGGTGTT
                     TNTSSGTTTSQK"
     gene            1095..1811
                     /locus_tag="AC16CY_03615"
     CDS             1095..1811
                     /locus_tag="AC16CY_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352895.1"
                     /translation="MPVSTFRQFGAWLDDTSAESPGGGYASIGIGHWRMLGSSQTNMP
                     MAGVGVGVTDRMQVGASVPYYHARFEGGSASGMDDVYLSAKYTIVDPTLTISEFGLAV
                     TPLVEVLSAGYPDGRVHFGLPVSMELRRQPFRVYGSAGWFSRGSLFGGAALEWSASRM
                     ILTGSLTNSYSLRDDAVLDSLGIGRRRADVSFGVAFPVADSVGAFANVGRSLTPVEEG
                     GTSLALSGGMTFRFSARATP"
     gene            1832..2230
                     /locus_tag="AC16CY_03620"
     CDS             1832..2230
                     /locus_tag="AC16CY_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352896.1"
                     /translation="MAPFSQAWLERYRDLLDADAEMRVIGGHFTLSMAVTFGDDRCVV
                     CFEKGRLVRWVLSPRIDVPSAFGFRSSREIWLKYLGRSPEPLYHDVFAMLMRVPGFVL
                     EGDTLVAMQHARALHRAMKVMQSVATGPHP"
     gene            2227..3072
                     /locus_tag="AC16CY_03625"
     CDS             2227..3072
                     /locus_tag="AC16CY_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3352897.1"
                     /translation="MRLEPIVGRYLTLRVGESDYRIFFEEAGQGVPLLCLHTAGADSR
                     QYRHLLNDEQVTSRFRVIAFDLPYHGRSTPPSGWWLQRYQLTKADYVAFIRAVWQALE
                     LARPVVLGCSMGGAVVLALAAEYQSELRGVVGLESTAFAPGRDNAFLHHPAIHGGEMT
                     ATYTYGLCAPQSPEDNKRENWWLYSQAGPGVYAGDVHFYSVDWDGRQAVTGIDTRVCK
                     VSLLTGEYDYSCTPEMTRQVAAAVPGSRMTIMKGIGHFPMIENYPLFREYLLAELEAI
                     VPGSA"
     gene            3069..>3276
                     /locus_tag="AC16CY_03630"
     CDS             3069..>3276
                     /locus_tag="AC16CY_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352898.1"
                     /translation="MNVPPRTFIRAALAVILLAVVSHTRADPDLWGHVRFGGDTVSEG
                     RLPDTDSYSFTADRAWINHEWLAEC"
BASE COUNT          536 a         1082 c         1091 g          567 t
ORIGIN      
        1 attcatcacc gatccgtggg ggacctacat cgagctgacc gaagggctgc gcgacgtgtc
       61 ctgaaagggg tcacgcccag cctgtaaccc ggtgtgcgaa ttcgacgatc ggagtcgggt
      121 cttcggcttc gatcccttct tgtaagtcgt tgcccaaccc acgcttgcgg cgcgttctct
      181 gagggcatgc tttttggact ggcatggccc tcggaggatc cgatcaatgc gaagattccc
      241 acttttcttt gtgactgcag cggcggttgt cgcactggcc acgtccggcg cggctcaggg
      301 gtccgggtcg ggcaaggggc cgaccaaggc gcccagcagc gcacacgtgc cgaagggcgg
      361 cgcatctggt tctggcgctg gtaagacgac caagacaacg ggcgggtcag cctccagcac
      421 cggcaagacg gccaagtcga cgggcgcctc gcacgctgga tcgaagaaga gcacgaccag
      481 caccgattcg acgacgacga cgacgaccac gaccggtacg ggtaccacgc ccacaacgaa
      541 cgcgctcgcg acgaagatct cgggcaaccc cgatcagctc gcgcgcgtgt cggccatgct
      601 gccggcggga gtgacgctcg aggacgcgac gaaaggcttc cgtaatcaag ggcagttcat
      661 cgcggcactg aacgcgtcga ggaaccggcc cggcatcgac ttcctggctc tccaggaggc
      721 gatgacggtc gatggtctca gcctgggtca ggcggtcaag aaggtgcaat cggctccggc
      781 gcctacggct ccgacgacgg gcggctccac gacggggacc ggcacgtcga cgggcaccga
      841 ctcgggcggc acgagcacga ccggtggcac cggcacgacg acgaacacct cctcgggcac
      901 gaccacgagc cagaagtaaa ggggggcccg tgacgaaccg cttcgctccc ccagcgattg
      961 ctctgctcgt tgctattggc ctcacgttcc ctgcgttcgc acacgctcag ggaaacgggc
     1021 gtcccaaatc ggcgaaggcg cccgccacta cgtccacaac gtccacggca tcggcgtcaa
     1081 ccacgttgac gccggtgccc gtatcaacct tccgtcagtt cggcgcatgg ctcgacgaca
     1141 ccagcgccga gagtcctggt ggcggctacg cgagcatcgg catcgggcac tggcgcatgc
     1201 tcggatcgag ccagaccaac atgcccatgg ctggcgtcgg ggttggcgtc accgatcgta
     1261 tgcaggtcgg cgccagcgtt ccgtactacc acgcacggtt cgaagggggc tcggcttcgg
     1321 gaatggacga tgtctatctc agcgcgaagt acaccatcgt cgatccgacg ctgactatca
     1381 gcgagttcgg tcttgcggtg actcctctcg tcgaggtgtt gagtgccgga tatccagacg
     1441 gccgtgtgca cttcgggctg cccgtgagca tggagctgcg ccggcagccc ttccgcgtgt
     1501 acggctcggc gggctggttc tcgcgggggt cgctttttgg cggggccgca ctcgagtgga
     1561 gcgcgtcaag gatgatcttg accggctcgc tcacgaactc gtattcgctc agggacgacg
     1621 cggtgctcga cagccttggc atcgggcgtc gtcgcgccga cgtctccttc ggtgtggcgt
     1681 ttccggttgc ggactcggtc ggcgccttcg cgaacgtcgg ccgcagcctg actcctgtgg
     1741 aagaaggggg aacgagtctg gcgctgagcg gcggtatgac gttcaggttc agcgcacgcg
     1801 caacgccctg acttgataag cttgccaccg cgtggctccg ttctcccagg catggctcga
     1861 acggtatcgg gatctgctcg acgctgacgc ggagatgcgc gtcatcggcg gccacttcac
     1921 gctctccatg gcggttacct tcggcgacga ccgctgtgtc gtctgcttcg agaagggccg
     1981 cctcgtccgc tgggtgctct cgccgcgcat cgacgttccg tcggcgttcg ggttccgatc
     2041 gagccgtgag atctggctga agtacctggg ccggtcgccc gagcccctct accacgacgt
     2101 cttcgcgatg ctcatgcgcg ttcctggatt cgtcctcgaa ggcgacacgc tcgtcgcgat
     2161 gcagcacgcg cgggcgctgc atcgcgccat gaaggtcatg caatcggtcg cgacggggcc
     2221 gcatccgtga ggctcgagcc gatcgtcggc cggtacctga cgcttcgcgt gggagagtcg
     2281 gactaccgta tcttcttcga agaagcgggg cagggcgtgc cgctgctgtg cctgcacacg
     2341 gcgggcgccg acagccgcca gtaccggcac ctcctcaacg acgaacaggt cacgagccgc
     2401 tttcgcgtga tcgcgttcga cctgccgtac cacggccgat cgacgccacc ctcgggctgg
     2461 tggctccaga gataccagtt gacgaaggcc gactacgtgg ccttcatacg ggccgtgtgg
     2521 caggcgctcg agctcgcgcg tccggtggtg ctcggctgct cgatgggcgg cgccgtcgtg
     2581 ctcgcgctcg cggccgagta ccagtcggag ctccgcggcg tcgtcggcct cgagagcacg
     2641 gcgttcgcgc caggccgcga caacgccttc ctgcatcacc cggcgatcca cggcggggag
     2701 atgacggcga cgtataccta cggcttgtgc gcgccgcaga gccccgagga caacaagcgg
     2761 gagaactggt ggctgtacag ccaggccggc ccaggcgtgt acgccggcga cgtgcatttc
     2821 tacagcgttg actgggacgg gcgccaggcc gtcaccggga tcgatacgcg cgtgtgcaag
     2881 gtctcgctgt tgaccggcga gtacgactac tcgtgcacgc cggagatgac acgccaggtg
     2941 gcggcggcgg ttcccggctc gcgcatgacg atcatgaagg gcatcggaca cttcccgatg
     3001 atcgagaact acccgctctt ccgcgagtac ctcctcgccg agctcgaggc catcgttccg
     3061 ggttccgcat gaacgtgccg ccgcgcacgt tcatccgggc ggctctggcg gtgatcctcc
     3121 tcgcggtcgt cagccacacg cgcgccgatc ccgatctctg gggacacgtg cggttcggcg
     3181 gcgacaccgt gtcggagggc cgcctcccag acacggactc ctattccttc accgccgatc
     3241 gagcctggat caatcacgag tggctcgccg agtgcg
//
LOCUS       DBNGSH010000157         2476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_860035, whole genome shotgun sequence.
ACCESSION   DBNGSH010000157 DBNGSH010000000
VERSION     DBNGSH010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_860035"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /gene="rplC"
                     /locus_tag="AC16CY_03635"
     CDS             <1..330
                     /gene="rplC"
                     /locus_tag="AC16CY_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR3352899.1"
                     /translation="FQGVVKRHHFKGGRATHGSMFHRAPGSIGASSYPSRVVKGMRMG
                     GHMGADRVTVRNLKVLRVDAENNLLLVEGAVPGGPNSVVVIRKAVAAKAIKVAQAEPA
                     KKKGKKA"
     gene            368..988
                     /gene="rplD"
                     /locus_tag="AC16CY_03640"
     CDS             368..988
                     /gene="rplD"
                     /locus_tag="AC16CY_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR3352900.1"
                     /translation="MTVDVVNSQNEKVGTLDLSDVVFGGRIKTDLIHESVVRSNAADR
                     RGTHATKTRAAVSGSGKKPWRQKGTGRARVGEIRNPLWRKGGTVFGPQPRSYEYKLPK
                     KVEKGALRAALSQKLRDGAVIVVDALSVAEIKTKAAAEMLKRLGVDGKALLVDVKPED
                     RLTLSIRNIEGVRLVASNRVSARDVVDTRRVVLTRAAIEKLQEALW"
     gene            995..1285
                     /locus_tag="AC16CY_03645"
     CDS             995..1285
                     /locus_tag="AC16CY_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR3352901.1"
                     /translation="MKLTDVIVRPVITEKTTVMREGTNTLVFEVAMKANKIDIRRAVE
                     QLLGSKVASVRTALAHGKEKRQGRSVGRRSDWKKAYVKLREGEKLPDFLQGA"
     gene            1289..2113
                     /gene="rplB"
                     /locus_tag="AC16CY_03650"
     CDS             1289..2113
                     /gene="rplB"
                     /locus_tag="AC16CY_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR3352902.1"
                     /translation="MPIRKYNPTSPGLRGKTSLTFDEITTSEPYRPLTENLHRSGGRN
                     NQGELTSWWRGGGHKRLYRIIDFKRDKKNIPGKIETVEYDPNRSARIALVTYADGEKR
                     YILQPMGMKVGDTVLAGDNIDILPGNCLPLKNIPLGTQIHNVELRPGKGGQIARSAGS
                     WVQLVAREGEYASVKMPSGELRKIHVECVATIGQVGNLDHENVSIGKAGRSRWLGKRP
                     HVRGVAMNPVDHPLGGGEGKTSGGRHPVSPWGQPTKGYKTRRRKQTDRFVIQRRPK"
     gene            2214..>2476
                     /gene="rpsS"
                     /locus_tag="AC16CY_03655"
     CDS             2214..>2476
                     /gene="rpsS"
                     /locus_tag="AC16CY_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR3352903.1"
                     /translation="MSRSVKKGPFVETALLEKIEAMNRANERKVVKTWSRRSTVVPEM
                     VGHTMAVHNGKKFIPVYVTENMVGHKLGEFAPTRLFKGHTTKT"
BASE COUNT          514 a          732 c          888 g          342 t
ORIGIN      
        1 ttccagggcg tcgtcaagag gcaccacttc aagggcggcc gcgcgacgca cgggtcgatg
       61 tttcaccgcg cgccgggctc gattggcgcc tcgtcgtatc cctcgcgagt cgtaaaggga
      121 atgcggatgg gcggccacat gggcgccgat cgcgtgacgg ttcgcaacct gaaagtgctg
      181 cgcgtggacg cggagaacaa cctgctcctg gtcgaaggcg ccgtgcccgg ggggccgaac
      241 agcgtggtcg tgatccgcaa ggcggtcgcg gccaaggcca tcaaggtggc gcaggcggag
      301 ccggccaaga agaaggggaa gaaggcctga ggcctgagcc ctgggccctg aggcctggtg
      361 taaagctatg actgtcgatg tggtgaattc gcagaacgag aaggtcggca cgctcgacct
      421 gagcgacgtc gtcttcggcg ggcgcatcaa gaccgatctg attcacgaat cggtcgtgcg
      481 cagcaacgcg gcggatcgtc gcggcacgca cgcgacgaag actcgcgcag ccgtcagcgg
      541 ctccggcaag aagccgtggc gccagaaggg gaccggccgg gcgcgcgtgg gcgagatccg
      601 caacccgctc tggaggaagg gcggcacggt gttcggcccg cagcctcgca gctacgagta
      661 caagctgccg aagaaagtcg agaagggcgc gctgcgcgcc gcgttgagcc agaagctccg
      721 cgacggcgcg gtcatcgtcg tggacgcact gagcgtggcc gagatcaaga cgaaggctgc
      781 ggccgagatg ttgaagcggc tcggcgtgga cggaaaggcg ctgctcgtgg acgtgaagcc
      841 cgaggaccgg ctgacgctct cgatccggaa catcgagggt gtgcggctcg tcgcgagcaa
      901 ccgcgtcagc gcccgggacg tggtggacac gcgccgggtc gtcctgacgc gggcagcgat
      961 cgaaaagctg caggaagcgc tctggtaggc acgtatgaag ctgactgacg tcatcgttcg
     1021 gccggtcatc accgaaaaga ccaccgtgat gcgcgagggg accaacacgc tcgtgttcga
     1081 ggtggcgatg aaggcgaaca agatcgacat ccgccgcgcg gtcgaacagc tgctcggatc
     1141 aaaagtggca agcgtgcgca ccgcgcttgc gcacggtaag gaaaagcgtc agggccgctc
     1201 ggtcggccgg cgctcggact ggaagaaggc gtacgtgaag ctgcgtgagg gcgagaagct
     1261 tcccgacttc ctgcagggag cgtagaacat gccgattcgc aaatacaacc cgacatcgcc
     1321 gggtctgagg ggcaagacgt cgctgacctt cgacgagatc acgacgagtg agccataccg
     1381 ccccttgacg gagaacctgc atcgctcggg tggccgcaac aatcagggcg agctgacgtc
     1441 ctggtggcgc ggcggcggac acaaacgcct gtaccgcatc atcgacttca agcgggacaa
     1501 gaagaacatc cccggcaaga tcgagacggt ggagtacgac cccaatcgat cggcgcgaat
     1561 cgcgctggtg acctacgcgg atggcgagaa gcggtacatc ctgcagccca tgggcatgaa
     1621 ggtcggggat acggtgctgg cgggcgacaa catcgatatt ctgccgggga actgcctgcc
     1681 cttgaagaac atccccctgg gcacgcagat tcacaacgtc gaattgcgac ccggcaaggg
     1741 tggtcagatc gcgcgcagtg ccgggtcatg ggtccaactc gttgcgcgcg aaggcgagta
     1801 cgcctcggtc aagatgccgt cgggcgagct caggaagatt cacgtcgagt gcgtcgcgac
     1861 gatcggccag gtcgggaacc tcgatcacga gaacgtgtcg atcggaaagg ccggccgcag
     1921 ccgctggctc gggaagcggc cgcacgtgcg cggcgtggcg atgaacccgg tggatcaccc
     1981 cctcggcggc ggagagggca agacctcggg aggccggcac ccggtgtcgc cctggggaca
     2041 gccgaccaaa ggctacaaga cgcgccggcg gaagcagacc gatcggttcg tcatacagcg
     2101 gagaccgaag taggccccag ggctcaggcc tcagggctca gggctgggcc aggccccagc
     2161 ctgatgcctg ctgaggcccg aggcctgggc cccgaggccg acaaggatca gatatgagtc
     2221 gttcagtcaa gaagggcccg ttcgtcgaga cagcgctcct cgagaaaatc gaggcgatga
     2281 atcgcgccaa cgagaggaag gtcgtgaaga cctggtcccg ccggtcgact gtcgtgccgg
     2341 agatggtggg gcacacgatg gcggtccaca acgggaagaa gttcatcccc gtgtacgtga
     2401 ccgagaacat ggtgggccac aagctcggcg agttcgcgcc gacgcgcctc ttcaaggggc
     2461 acacgacgaa gacgga
//
LOCUS       DBNGSH010000158         2680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2218325, whole genome shotgun sequence.
ACCESSION   DBNGSH010000158 DBNGSH010000000
VERSION     DBNGSH010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2218325"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1021
                     /locus_tag="AC16CY_03660"
     CDS             176..1021
                     /locus_tag="AC16CY_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006517753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3352904.1"
                     /translation="MQTVALTAAALVAFAGNSLLCRMALGQGSIDAASFSTIRLIAGA
                     CSLAVISAAQGSRARASAGAWTSAAALFLYAIPFSFAYVWLPTGTGALILFGAVQVTM
                     MLGALAGGERPRARQWTGLVLAVAGLVYLGLPGLAAPPLAGAALMAVAGIAWGVYSLR
                     GRGAANPLGQNAGSFARSIPMALVVSILAFEQRHLEARGVLLAVASGALASGLGYVAW
                     YSALRSLSATRASLVQLLVPILAAAGGIAFLGEPLSLRLVLSAVIVLGGIALALWKSG
                     AMRSG"
     gene            1073..1996
                     /locus_tag="AC16CY_03665"
     CDS             1073..1996
                     /locus_tag="AC16CY_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HIR3352905.1"
                     /translation="MSKRAFWLCPLVAVFAAAALPSAQSPAKPARINRAIEMLEKGQP
                     VYYTGGSGGYEEGKKLAQTPSDYINYEMEHGAFDISALRAFMQGLVDGGPAATGHRTP
                     AVIVTLPVLGLDEASMRANYWVVHQVLAAGVHGILLCHARVPEAARLLVEATRYPFAP
                     KVAGLGEGLRGSGSQGYAARIWGIPGNEYLRRADPWPLNKEGELLLGVKIEDSHALAN
                     AERTTKVPGISFAEWGPGDMGFWLLGIPQPGQTDLPPMRAARARVFQATRDAKIFFLN
                     TCNDKNVVEMIKEGVMICTGGFEVGRKFTAR"
     gene            complement(1983..>2680)
                     /locus_tag="AC16CY_03670"
     CDS             complement(1983..>2680)
                     /locus_tag="AC16CY_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352906.1"
                     /translation="PPKPAPRSADGRVLLGGATPAEKGVWLPRGVAANPLGLKDPPFQ
                     PWAKAVFVDRGENELEPHTRCKPSGVARQFLTPYGVEFVELSELQRIYVFDIGGPHTY
                     RTIFMDGRTHPANITPTYYGHSIGWWEGDTLVIDTTGYNEGFWLDRDGLPHTEKLRTL
                     ERITRADASTLKYELTVDDPGAYTAPWSGTMNLQWEAGTELFEYVCQQSNFAHDLMLG
                     RYQSVDRTTPIVP"
BASE COUNT          377 a          905 c          940 g          458 t
ORIGIN      
        1 ttcaccgatc ggcaggcgct cgccgacgcg agggcggaat atcgcaagcg cgtcgggccg
       61 gatttcacct accgtgccct ggtgggcgat cgcaagccgc cgctcgacta tcgcggcaag
      121 tgatcgaacg cgagggtcta gggctgcttc gaggcggacg catcacacct gacgcgtgca
      181 gaccgtcgct cttaccgccg cggcgctcgt cgccttcgcc ggcaactcgc tcctgtgccg
      241 gatggcgctc gggcagggca gcatcgacgc ggcgtcgttc tcgacgatcc gtctgattgc
      301 cggcgcgtgt tcgctcgcgg tgatctcggc cgcgcaaggg tctcgcgccc gggcgtcggc
      361 cggcgcgtgg acgtcggccg cggccctctt tctctacgcc atcccgttct cgtttgccta
      421 cgtgtggctt ccaaccggaa caggcgcgct gattctcttc ggcgccgtgc aggtcacgat
      481 gatgctgggc gccctggccg ggggagagcg gccgcgcgcg cggcagtgga ccgggctcgt
      541 gctcgcggtg gccgggctcg tgtacctcgg gcttccgggg ctcgccgcgc cgccgttggc
      601 cggcgcggcc ctgatggccg tcgcgggcat cgcgtgggga gtctactcgc tgcgcggccg
      661 cggcgccgcg aacccgctgg gtcagaacgc cggcagtttc gcccgttcga ttccgatggc
      721 gctcgtcgtc agcatcctcg cgttcgagca gcgtcacctg gaggcgcgcg gcgtgctgct
      781 cgcggtcgcg tcaggggcgc tcgcctccgg cctcggttac gtggcgtggt attcggccct
      841 ccggtctctc agcgcgacgc gcgcctcgct cgtgcagctg ctcgtgccga tcctcgccgc
      901 cgcgggaggc atcgcgtttc tcggggagcc gctgtcgctt cgactcgtcc tctccgccgt
      961 gatcgtcctg ggcgggatcg cgctggcgct ctggaagtcc ggggccatgc ggtccggcta
     1021 gagccttaga ctgcgaccgg cagtatcatc tcgtccgaag ggggaggatg ccatgtcgaa
     1081 gcgcgccttc tggttgtgtc cgctcgtcgc cgtcttcgcc gcggccgctc tgccatccgc
     1141 tcaaagcccg gcgaagccgg cccgcatcaa cagagcgatc gagatgctcg agaaagggca
     1201 gcccgtgtac tacacgggag gcagcggcgg ctacgaggaa ggcaagaagc tcgcgcagac
     1261 gccgtccgac tacatcaact acgagatgga gcacggcgcc ttcgacatca gcgcgctgcg
     1321 cgcgttcatg caggggctcg tcgatggcgg gcccgccgcg accgggcacc gcaccccggc
     1381 cgtgatcgtg acgctgcccg tgctcggcct ggacgaggcc agcatgcgcg ccaactactg
     1441 ggtcgtccat caggtgctcg ccgccggcgt ccacggcatc ctgctctgcc acgcgcgcgt
     1501 gcccgaggcg gcgcggctgc tcgtcgaggc gacgcgatat ccgttcgcgc cgaaggtcgc
     1561 cgggctgggt gagggtcttc gcggcagcgg gagccagggc tacgccgcgc gcatctgggg
     1621 aattcccggc aacgagtatc tccgtcgcgc cgatccgtgg ccgctcaaca aggaagggga
     1681 gcttctgctc ggcgtcaaga tcgaggacag ccacgcgctc gccaacgccg aaaggacgac
     1741 aaaggtgccc ggcatctcgt tcgcggagtg gggacccggc gacatggggt tctggctcct
     1801 cgggatcccg cagccggggc agacggacct gccccccatg cgcgcggcca gggcccgcgt
     1861 ctttcaggcg accagggacg cgaagatctt cttcctgaac acgtgcaacg acaagaacgt
     1921 cgtggagatg atcaaggaag gcgtgatgat ctgtacgggc ggcttcgagg tcgggaggaa
     1981 gtttacggca cgataggcgt cgtgcgatcg accgactggt accggccaag catcaggtcg
     2041 tgggcgaagt tcgactgctg gcacacgtac tcgaacagct cggtgcccgc ctcccactgg
     2101 aggttcatcg ttcccgacca gggcgcggtg taggcgccgg ggtcgtcaac cgtcagctcg
     2161 tatttcagcg tcgaggcgtc ggcccgcgtg atgcgctcga gcgtcctgag cttctcggtg
     2221 tgcggcaggc catcgcggtc cagccagaac ccttcgttgt agcctgtcgt gtcgatcacg
     2281 agcgtgtcgc cctcccacca tccgatcgag tggccgtaat aggtcggcgt gatgttcgcc
     2341 ggatgcgtac gtccgtccat gaagatggtg cggtaggtgt gcggtccgcc gatgtcgaag
     2401 acgtagatcc gctgcagttc ggacagctcc acgaactcga caccgtaggg cgtcaggaac
     2461 tgacgcgcca cgcctgaggg cttgcagcgc gtgtgcggct cgagctcgtt ctcgccgcga
     2521 tccacgaaga cggctttcgc ccacggctga aaagggggat ctttcaggcc gagcgggttc
     2581 gcggcaactc cgcgcggcag ccacacgccc ttctcggcgg gcgtcgcccc gccgagcagc
     2641 acgcgtccgt cagccgatcg aggcgccggc ttcggcgggc
//
LOCUS       DBNGSH010000159         1589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2897741, whole genome shotgun sequence.
ACCESSION   DBNGSH010000159 DBNGSH010000000
VERSION     DBNGSH010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2897741"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="AC16CY_03675"
     CDS             <1..632
                     /locus_tag="AC16CY_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3352907.1"
                     /translation="YGPEEVVRAQWDHTKRRLSSIPNVRFQDGAFYRFPLTEEQKRSV
                     RDPQDLGIPTLETFSIGARSNFAPATRGHLGFSPVIPMTGEAVIESQRVFQRVLQQFK
                     TDVALLVFPQSYHPRTLVILCMFYVMPETQHNQAVRATFRRLVEVAAENGWGEYRTHT
                     AFMDECARAYAFNDNALLRLQERIKDAVDPKGILSAGRYGIWPRHLRRG"
     gene            743..1117
                     /locus_tag="AC16CY_03680"
     CDS             743..1117
                     /locus_tag="AC16CY_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3352908.1"
                     /translation="MIQSTRLAAALLMVAAALAPGASGAAQTGATAAPDKALLERGKA
                     VYNEWCAACHDPGPRHPGTQALDALYRGKKPGALEQRTDLVPTLTETFVRRGVSVMPP
                     FRKTEISDADLAALAAYLAPRP"
     gene            1169..>1589
                     /locus_tag="AC16CY_03685"
     CDS             1169..>1589
                     /locus_tag="AC16CY_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HIR3352909.1"
                     /translation="MKRREFLAGIAAAPLVRSVRLEPDRNAVHASPLASQAAPAARAR
                     APIRQSVMASVWGTGSALSFEERCKILAKIGFKGVDLPTAQQVPILKQYGLTPAMMTG
                     TGTSFQEGLIRKELHGKFEDAFRAGIDMCAEVGCPNLI"
BASE COUNT          267 a          530 c          546 g          246 t
ORIGIN      
        1 tctacggtcc ggaagaggtc gtgcgcgcgc agtgggacca cacgaagcgg cggttgtcgt
       61 cgattccgaa cgtacgcttc caggacggcg cgttctatcg cttcccgctg accgaggagc
      121 agaagcggag cgtccgcgat ccgcaggacc tgggcatccc gacgctcgag accttctcga
      181 ttggcgcccg gtcgaacttc gcgcctgcga cgcgggggca cctgggcttt tcgcccgtga
      241 tcccgatgac gggcgaggcg gtcatcgagt cgcagcgcgt gttccagcgc gtgctgcagc
      301 agttcaagac cgacgtggcg ctgctggtgt ttccgcagtc gtaccacccg cgcacgctcg
      361 tcatcctgtg catgttctac gtgatgccgg agacgcagca caaccaggcg gtccgcgcga
      421 cgttcaggcg gctcgtggaa gtggcggccg agaacggatg gggtgagtac cgcacccaca
      481 cggcgttcat ggacgagtgc gcgcgcgcct atgcgttcaa cgacaacgcg ctgctgcgcc
      541 tgcaggagcg gatcaaggac gccgtcgacc cgaagggcat tctctctgcc ggccgatacg
      601 gcatctggcc tcgccacctg cgtcgaggat gacgcccatg tcgcgcacta ctggagcgca
      661 tcagcgaaac gtaaagcggg ccgtccgttg gttggcccgc gaaatcggcg tcggagggct
      721 gtccgactcg ctacaggttc aattgataca gtccactaga cttgccgccg ccctgctgat
      781 ggttgccgcc gcgctcgcgc ccggcgcgag cggagccgcg cagaccggag ccacggccgc
      841 cccggacaag gcgctgctcg agcgggggaa ggcggtctac aacgaatggt gcgcggcatg
      901 ccacgacccc ggaccgcgcc atccgggcac gcaggctctc gacgcgctct accggggaaa
      961 gaagccgggg gcgctcgagc agcggacgga tctggtgccg acactcacgg agacgttcgt
     1021 ccgtcgaggc gtctccgtga tgccgccgtt ccgaaagacg gagatcagcg acgccgatct
     1081 cgcggcgttg gccgcctacc tggctccccg gccctgagcc gcgacccgca gcatggcggg
     1141 gctcccgcaa cggggttata gtgcccgcat gaagagacgc gaattcctcg ccggtatcgc
     1201 cgcggcgcct ctggtacgta gtgtccggct tgagccggac cgtaacgccg tacacgcctc
     1261 accgctggcg tcacaagccg cacccgccgc ccgggcgcgg gcgcccatca ggcagagcgt
     1321 gatggcatcg gtctggggaa ccgggtcggc gctctccttc gaggagcggt gcaagatcct
     1381 cgcgaagatc ggattcaagg gcgtcgatct gcccacggcg cagcaggtgc cgatcctgaa
     1441 gcagtacggc ctgacgccgg cgatgatgac cggcaccggc accagctttc aggaggggct
     1501 gatccggaag gaactgcacg gcaagttcga ggacgcgttc cgcgccggca tcgacatgtg
     1561 cgccgaggtc ggctgtccga acctgatcg
//
LOCUS       DBNGSH010000160         1837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2048910, whole genome shotgun sequence.
ACCESSION   DBNGSH010000160 DBNGSH010000000
VERSION     DBNGSH010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2048910"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="AC16CY_03690"
     CDS             complement(<1..773)
                     /locus_tag="AC16CY_03690"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HIR3352910.1"
                     /translation="MKAIVVREFGPPEVMRLEDVTDPAAGPGQVVIRVRAVGVNPVDT
                     YIRAGAYARKPSLPYTPGTDVAGQVHAVGAGVTRVRTGDRVYAHGAVGGCAEFALCDD
                     WRVCPLPDRLTFPRGAAVGVPYGTAWRALLMRAQARAGETVLVHGASGGVGTAAVQIA
                     RAHGMRVIGTAGTKEGLALVTESGAHHTLNHRDADYLQQVMPLTGGKGVDVVLEMLAN
                     VNLDRDLDVLAPRGRVVVIGNRGRVEIDPRKTMARDAAIL"
     gene            870..>1837
                     /locus_tag="AC16CY_03695"
     CDS             870..>1837
                     /locus_tag="AC16CY_03695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3352911.1"
                     /translation="MVHPALVFTDRPEQAHRIQRYFIGVASSVLVWALMFLLYAQGHI
                     EATALVRAGVAMLFLFVAFYALFRTHVNLRASDPSLTSAQILSSIAVTLYAMYFTSSE
                     ARTLLSLVFIMSFFFGVFRLGTRQFAMIAALTVAGYLVVIGLLARNRAHAVDLNLELM
                     RCAATAAVLFWFSVVGGYLSRLRKRLADGRAKLEKAVRTIKEQVDTDELTGTHSRRYL
                     LEVLERERSHSRRTGGTFCVCIADIDRFKQINDTFGHHAGDQVLRSLTRAAQRGLRVT
                     DYFGRYGGDELMLVLTGSTLEGALLRAEQVRARIEHLTFPDLDPVV"
BASE COUNT          285 a          634 c          603 g          315 t
ORIGIN      
        1 agaatcgcgg catctcgcgc catcgtcttg cgcggatcga tctccacgcg cccgcggttg
       61 ccgatgacga ccacgcggcc gcggggcgcg agcacgtcga ggtcgcgatc gaggttgacg
      121 ttggcgagca tctcgaggac gacgtccacg cctttcccgc cggtgagcgg catcacctgc
      181 tggaggtagt cggcatcacg gtgattgagc gtgtggtgcg cgccgctctc cgtgacgagc
      241 gccaaccctt ccttcgtgcc ggccgttccg atgacgcgca tgccgtgcgc gcgcgcgatc
      301 tggaccgccg ccgtgccgac cccgccgctc gcgccgtgca ccaggacggt ttcgcccgcg
      361 cgcgcctgcg cgcgcatcag cagcgcccgc cacgccgtgc cgtacggcac gccgacggcg
      421 gcgccccgcg gaaacgtcag gcggtccggc agcggacaga cccgccagtc gtcgcatagg
      481 gcgaactcgg cgcagccgcc cacggcgccg tgggcataca cgcgatcgcc tgtgcgcact
      541 cgcgtgacgc cggcgccgac ggcgtgcacc tgtccggcga cgtccgtgcc gggtgtgtaa
      601 ggcaggctgg gttttcgcgc gtacgccccc gcgcggatgt acgtgtcgac cgggttgacg
      661 cccaccgcgc gaacgcgaat gacgacctgg cccggccctg ccgctggatc cgtcacgtct
      721 tcgagccgca tcacctcggg gggaccgaac tcgcgaacga cgatggcctt catgggaacg
      781 gatcttactc gcttcgggag cgacgggacg aagacgcaac gctcaggcta tcgacaacgg
      841 ccggccggcc gaaacgaaga tggagccacg tggtccatcc tgcactcgtc ttcaccgatc
      901 gtcccgaaca ggcacaccgg attcagcgtt acttcatcgg cgtggcatcg tcggtcctcg
      961 tctgggccct gatgttcctg ctctatgcgc aggggcacat tgaggcgacg gcgctcgtac
     1021 gtgccggtgt ggccatgctg ttcctgttcg tggccttcta cgccctgttc cgcacgcacg
     1081 tgaacctccg cgcatccgat cccagcctga cgagcgcgca gatcctgagc tcgattgccg
     1141 tcacgctcta cgcgatgtac ttcacatcca gcgaagcgcg gacgctgctc tcgctcgtgt
     1201 tcatcatgtc cttcttcttt ggtgtgtttc gcctcggcac gaggcagttc gcgatgatcg
     1261 cggcgctgac cgttgcggga tatctcgtcg tgatcgggct gctcgctcgc aatcgggctc
     1321 acgccgtcga tctcaacctc gagctgatgc ggtgcgcggc cacggccgcg gtgctgttct
     1381 ggttctcggt ggtcggaggt tacctcagcc ggctgcgcaa gcgactggcc gacggccgcg
     1441 cgaagctcga gaaggcggtg cgtacgatca aggagcaggt ggacaccgac gagctcacag
     1501 ggacccacag ccgccgctac ctgctggaag tgctcgagcg ggagcgatcg cacagcagac
     1561 gtaccggagg aaccttctgc gtctgcatcg ccgatatcga caggttcaag cagatcaacg
     1621 acaccttcgg acatcatgcc ggagaccagg tgctgcgcag cctcactcgc gcggcgcaga
     1681 gagggctgcg cgtgaccgac tacttcggcc gctacggcgg ggacgagctg atgctggtct
     1741 tgaccggcag cacgctcgaa ggcgccctcc ttcgagccga gcaggtccgg gcccggatcg
     1801 agcacctgac gtttcccgac ctcgatccgg tcgtgaa
//
LOCUS       DBNGSH010000161         2719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3067972, whole genome shotgun sequence.
ACCESSION   DBNGSH010000161 DBNGSH010000000
VERSION     DBNGSH010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3067972"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /locus_tag="AC16CY_03700"
     CDS             complement(<1..2281)
                     /locus_tag="AC16CY_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3352912.1"
                     /translation="MARSGNVGLIRALFVLFCAALAPTDREAEAQAASGLSGTVVARG
                     TSQPVADATVTAEGSGATAATNALGRFELPTGAAPVTLVVRARGYLELKLPGVQAQTG
                     LVIELDPTPNFLESVQVTATKSALAAGDVAAPTTSVSRQTIDLRGDQRLTEAVEHVPG
                     ALISTELGIFENVLLRGMPRQGNEFTNTLLLIDGVPQTNSGNEARVVALPINDARSIE
                     IVRGPNSALYGRTAIGGAINLLTADPTAIPELRVDLAGGQFGTARGVVAASGPIARWG
                     GFYASVGTEHGGGYFNDLVDPDYDTGNVALFGKLKFTTSPRSFGTVTINRVSSNNSTP
                     TNEPVVDGQFLHEIDPQFDRLTNLNLPGPTYSQGETRFTANYNWQFSDWGRFVETFGY
                     RDVRLEFIEDGDFIGEPYNLTNQTVTMYPFSQELNESVFYQEARLEAGGTSGSVRHDA
                     TVGWSYEDNSGDLWADFIYTDEDLFGFPDISYVNPVIPDRSVWQHDEATRVYNLGITG
                     IFGQYMVQPTSRLLLTAAGRYDRLNMDATRGTGVTVEQTFDAFSPKVSATYRFFGNDS
                     GRALNAYGAYSQAFNPPRRPSALIPADIPLNLQPVDIENWETGIKGSLADGAVSLEAS
                     YFHMTEDGVVLSQRQGAFFLPTNAGQRKYDGFETGLGWSASSKISTYVNASFYHHRFG
                     DFVIESQEGDLDLEGNHLVLSPDYIVNWGAVFQITHAIDARFDVKHVGPTFGDDENSY
                     RIPGYSLVDVAASWLHGSGL"
     gene            complement(2385..>2719)
                     /locus_tag="AC16CY_03705"
     CDS             complement(2385..>2719)
                     /locus_tag="AC16CY_03705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3352913.1"
                     /translation="DLFLLPSSQESFGLAALEAMACEVPVIASRVGGLPEVIEHGVSG
                     FLHPLDAIDDMAASGVTLLTNDTLHHDVSQAACRRVRSDFSVDRVVPMYEACYRELLL
                     PGPSLSQP"
BASE COUNT          462 a          864 c          915 g          478 t
ORIGIN      
        1 gcagaccgga cccgtggagc cacgacgccg cgacgtccac gagcgaatag ccggggatgc
       61 ggtacgagtt ctcgtcgtcg ccgaaggtcg gcccgacgtg cttgacgtcg aagcgcgcgt
      121 cgatggcgtg agtgatctgg aacaccgcgc cccagttcac gatgtagtcc ggcgacagaa
      181 ccaggtggtt cccttcgagg tcgaggtcgc cctcctgcga ctcgatcacg aaatcgccga
      241 agcgatggtg atagaacgac gcgttgacgt aggtcgagat ctttgacgag gccgaccagc
      301 caagcccggt ctcgaaaccg tcgtacttcc tctggccggc gttggtcggg aggaagaagg
      361 ccccctgccg ctggctcagt acgacgccgt cctcggtcat gtggaagtac gacgcctcga
      421 gcgagacggc gccgtccgcc aggctgccct tgatgccggt ctcccagttc tcgatgtcca
      481 ccggctgcag gttcaacgga atatcggcgg gtatcagggc gctcggccgg cgggggggat
      541 tgaacgcctg cgagtaagcg ccgtaggcgt tgagcgcacg gccgctgtcg ttgccgaaga
      601 acctgtacgt ggcgctgacc ttcgggctga acgcgtcgaa ggtctgctcg acggtgaccc
      661 cggtgccgcg cgtcgcgtcc atgttcagcc tgtcatagcg tcctgcggcg gtcaggagga
      721 gtctggacgt cggctgaacc atgtactgcc cgaagatccc cgttatcccg aggttgtaga
      781 cgcgggtggc ctcgtcgtgc tgccacacgg accggtcggg gatgacggga ttcacgtagc
      841 tgatgtccgg gaagccgaag agatcctcgt cggtgtagat gaagtcggcc cacaggtcgc
      901 cgctgttgtc ctcgtaagac cagccgacgg tggcgtcgtg acggaccgat ccgctggtcc
      961 cgcccgcctc gagccgcgct tcctgataga acacactctc gttgagctcc tgactgaacg
     1021 gatacatggt caccgtctgg ttcgtcaggt tgtacggctc gccgatgaag tccccgtctt
     1081 cgatgaactc gagccgcacg tcccgatagc cgaacgtctc cacgaacctg ccccagtcgg
     1141 agaactgcca gttgtagttg gcggtgaagc gcgtctctcc ctgggagtac gtcggtcctg
     1201 ggaggttgag gttcgtcaac cgatcgaact gcggatcgat ttcgtggaga aactggccgt
     1261 cgaccaccgg ctcgttcgtc ggcgtgctgt tgttggacga cacacggttg atcgtcaccg
     1321 tgccaaacga cctcggcgag gtcgtgaact tcagcttgcc gaacagagcg acgttgccgg
     1381 tgtcgtagtc ggggtccacc aggtcgttga agtatccgcc gccgtgctcc gtgccaacgg
     1441 aagcgtagaa cccgccccat cgcgcgattg gtcccgacgc agcgacgacg cccctcgcgg
     1501 tgccgaactg gccgcccgcg agatcgaccc tgagctccgg tatcgccgtc gggtcggcgg
     1561 tcaacaggtt gattgcgcct ccgatcgccg tgcgaccgta gagcgcggag ttcggtccgc
     1621 ggacgatctc gatgctgcgc gcatcgttga tcggcagggc gacgacgcgc gcctcgttgc
     1681 cggaattggt ttgcggcacc ccgtcgatga gcaggagcgt gttcgtgaac tcgttgccct
     1741 ggcggggcat accgcggagg agcacgttct cgaagatgcc gagctcggtc gagatgaggg
     1801 cacctggcac gtgctcgacg gcctcggtca gtctctggtc gccccgcaga tcgatcgtct
     1861 gacgtgacac gctggtggtc ggcgcagcga cgtcgcccgc ggccagggcc gacttggtcg
     1921 cggtcacctg gaccgactcg aggaagttcg gcgtcggatc gagctcgatg acgaggccgg
     1981 tctgcgcctg aacgccaggg agcttcagct ccaggtaacc gcgcgcgcgc acgacgagcg
     2041 tgaccggcgc agccccggtg ggcagttcaa agcggcccaa cgcattggtc gctgccgtcg
     2101 cccccgaacc ctcggcggtg acagtggcgt cggcaacggg ttggctggtc ccacgagcga
     2161 ccaccgttcc ggacagtccg ctggcggcct gggcctccgc ctcgcgatcg gtgggggcaa
     2221 gcgccgcgca gaacagcacg aacagtgccc ggatcagtcc aacgttgcca ctccgcgcca
     2281 tgaaggtctc tccctttcga ctgctcgcag gcggcgagct gtggagcggg tgagcttaac
     2341 agcgtgcgga gaggcggtca tctgaagagt tcctgaattc caagtcaggg ttgagatagg
     2401 gacggtcccg gcaacagcag ctcgcgataa caggcctcgt acatcggtac cacgcgatcg
     2461 accgagaagt cgctccgcac tcggcgacac gccgcctggc tcacgtcatg atgcagggtg
     2521 tcgttcgtca acagcgtcac gccgctggcc gccatgtcgt cgatcgcgtc gagcgggtgg
     2581 agaaacccgc tgacaccgtg ctcgatgact tcgggcagcc cgccgactct cgacgcgatg
     2641 accggcacct cgcacgccat cgcctcgagg gcggcgaggc cgaagctctc ctgcgaggac
     2701 ggcagcagga acaagtcgg
//
LOCUS       DBNGSH010000162         1728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3747276, whole genome shotgun sequence.
ACCESSION   DBNGSH010000162 DBNGSH010000000
VERSION     DBNGSH010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3747276"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="AC16CY_03710"
     CDS             complement(<1..482)
                     /locus_tag="AC16CY_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3352914.1"
                     /translation="MSDRRPILVTGATGFAGGHLVEHLAGTGRVIGWGRSAPVRELAH
                     VAAWQTVDLLDREEVSRAIATLRPASVYHLAGATQVAESWQDTARPLRSNVLATAHLL
                     DAIRREKVDCRILLSGSSAVYAPSDAPVREDASLAPASPYALSKLAQEQLALRACRDD
                     R"
     gene            complement(483..1493)
                     /gene="rfbB"
                     /locus_tag="AC16CY_03715"
     CDS             complement(483..1493)
                     /gene="rfbB"
                     /locus_tag="AC16CY_03715"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR3352915.1"
                     /translation="MVEVLVTGGAGFIGSNFVRYALEQHRDWRVTTLDKLTYAGRREN
                     LHDVMDDPRHTFVHGDIADPSVSAPLVERSHLVVHFAAETHVDRSIMGAGDFIRTDVE
                     GTFVLLEAARRAKNLRRFVQISTDEVYGSVPSGASVETDELRPRNPYAASKAGADRLA
                     YSYWATYDLPVIITRASNNYGPYQFPEKVIPLFVTNAIDGIPVPLYGDGRNVRDWLHV
                     LDHCRAIDLLIDRASHGEVYNIGGGNDIMNVDLTHRILDALGKPHSLIKPVADRPGHD
                     RRYCLDTTKLRQLGWAPQAPFAEGLRDTVAWYRDNEWWWRPIKERDPAFRAYYQAQYE
                     GR"
     gene            complement(1486..>1728)
                     /locus_tag="AC16CY_03720"
     CDS             complement(1486..>1728)
                     /locus_tag="AC16CY_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIR3352916.1"
                     /translation="LVDTRPDSPTNGAINEFFLGTQNPTLVQVPNLVYHGWKCISPEV
                     SLVVNVPNEPYRYADPDEFRLEPHHSLPYDWTRKDG"
BASE COUNT          267 a          554 c          608 g          299 t
ORIGIN      
        1 cggtcgtctc tgcacgcgcg cagcgccagc tgctcctgcg ccagcttgct gagcgcgtaa
       61 gggctggcgg gcgcgagcga cgcatcctcg cggacagggg cgtctgaagg cgcgtacacc
      121 gccgacgagc ccgacagcag gatccggcag tccaccttct cgcgccggat ggcatcgagc
      181 aggtgcgccg tggcgagcac gttgcttcgg agtggcctgg ccgtgtcctg ccacgactcg
      241 gcgacctgcg ttgccccagc caggtggtag accgaggccg gcctgagggt cgcgatggcc
      301 cgcgagacct cttcccggtc gagcaggtcg accgtctgcc atgcggcgac gtgcgccagc
      361 tccctgaccg gcgccgatcg gccccatccg atcacgcggc cggtgccggc aaggtgttcg
      421 accaggtggc cgccggcgaa gccggtcgcg ccggtgacca ggattggccg gcgatccgac
      481 acttaccggc cttcgtattg ggcctggtaa tacgcgcgaa aggccgggtc gcgctccttg
      541 atcggcctcc accaccactc gttgtcgcgg taccaggcga cggtgtcgcg cagcccttcg
      601 gcaaacggcg cctgcggcgc ccacccgagc tggcgcagct tggtcgtgtc caggcaatag
      661 cgtcggtcgt ggccgggacg atcggcgaca ggcttgatga gcgagtgagg cttgcccagc
      721 gcatcgagaa tccggtgggt gagatcgacg ttcatgatgt cgttgccccc gccgatgttg
      781 tagacctcgc catgcgacgc acgatcgatg agcagatcga tcgcgcggca gtggtcgagg
      841 acatgcagcc agtcgcggac gttgcggccg tcgccgtaga gcggcacggg gatgccgtcg
      901 atcgcgttgg tcacgaagag cgggatcact ttctccggga actgatacgg gccgtagttg
      961 ttcgacgcgc gcgtgatgat gacgggcagg tcgtaggtgg cccagtagct gtacgccagc
     1021 ctgtcggcgc ccgctttact cgcggcgtac gggtttcgag gcctgagctc gtcggtctcg
     1081 acgcttgccc ccgacgggac gctgccatac acctcgtcgg tcgagatctg cacgaagcgc
     1141 cgcaggttct tggcgcggcg cgccgcttcg agaagcacga acgtcccctc gacgtcggtc
     1201 cggatgaaat caccggcgcc catgatcgag cgatcgacgt gggtttcggc ggcaaagtgc
     1261 accacgagat gggaacgttc gacgagcggt gcgctgacgc tcgggtccgc gatgtcgccg
     1321 tgcacgaacg tatggcgcgg gtcgtccatc acgtcgtgaa ggttctcgcg ccggcccgcg
     1381 taggtcagct tgtcgagcgt cgtcacgcgc cagtcgcgat gctgttcgag cgcgtagcgg
     1441 acgaagttgc ttccaatgaa gccggcgccg ccggtgacca ggacttcaac catccttgcg
     1501 cgtccagtcg taggggagcg agtgatgcgg ctcgaggcgg aactcgtcgg gatcggcgta
     1561 ccgatacggc tcgttcggca cgttgacgac gagcgacacc tcggggctga tgcatttcca
     1621 cccgtggtag acgagattcg gcacctgcac gagcgtcggg ttctgcgtcc cgaggaagaa
     1681 ctcgttgatc gcgccgttgg tcggcgagtc cggcctcgtg tccacgag
//
LOCUS       DBNGSH010000163         2387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_860672, whole genome shotgun sequence.
ACCESSION   DBNGSH010000163 DBNGSH010000000
VERSION     DBNGSH010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_860672"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="AC16CY_03725"
     CDS             complement(<1..932)
                     /locus_tag="AC16CY_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352917.1"
                     /translation="MKKKLSGWQMAGLVAGIGCFTIVVVVAVGVAVAVSWARSAVAEL
                     GDPTPANHTRTVTLGPPSAGDPAQAGGASGAAAKGAGSKSGDPLRLTVELAEGDFTIR
                     KGPAGGQVQVEGTYAEGMYELTENTAADEAGHSRTTIRFKSKAPLWARILGGMGGEGP
                     NRPRLTVLIPAGVPIDLSLRVGMAESRIDLGGLTLSDLGIDLSMGNHILDFKEPVVGG
                     LRRVRLNASMGNLTVDNLGNTGAEAIEATGSMGNFTADLGGAWQPESNAELNFSQSMG
                     QLTLQVPSSVRLDANITNSQGGTTSSPNSDEETK"
     gene            1109..1996
                     /locus_tag="AC16CY_03730"
     CDS             1109..1996
                     /locus_tag="AC16CY_03730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3352918.1"
                     /translation="MLNELSNHVADVVAAVAPSVVQVHGRRRPASGLVYADGLILTTV
                     RAIGGEDGIRVRQHDDVDVEAELAGWDAATSLALVRVSGVTAPPLRPASSPPRVGNLA
                     IAVARSWSNSVTASAGIVSVIGGPFRTGRRRAIDQIIRTTAPMHDGFSGGAFIDSTGA
                     LIGVTTAAAIRGLGVVIPAGIAWATADRLAQHGHARRGYLGVLGQPVALSASQSAAAG
                     RDKAMLVVGVSHGSAAAQAGVLVGDILLAFEGTPTSSPEDLLDLLADAQAGRQATLHV
                     LRGDKPVDVRVTVGERPGR"
     gene            1997..>2387
                     /locus_tag="AC16CY_03735"
     CDS             1997..>2387
                     /locus_tag="AC16CY_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3352919.1"
                     /translation="MRAILVGPPAARERLRLELESRAIDVIGEFESEIDARASGLSAD
                     LIVSAADSMPRERGNGDDAIQEPLTPREIEVLELLAEGLPNKGIAARLGISDQTVKFH
                     VAAIMGKLGASNRTDAVRRAVRRGLVSL"
BASE COUNT          324 a          807 c          834 g          422 t
ORIGIN      
        1 tccttggttt cctcgtcgct gttcgggctg ctcgtcgtcc cgccctgcga gttggtgatg
       61 ttcgcgtcga gccgcacgct gctcgggact tgcagcgtca gctgacccat cgactggctg
      121 aagttcagct cggcgttgga ttcgggttgc cacgcgcccc cgaggtcggc cgtgaagttg
      181 cccatgctgc cggtggcctc gatcgcctcc gcgccggtgt tccccaggtt gtctacggtg
      241 aggttcccca tgctcgcgtt gaggcggacg cgccgcagcc cgccgacgac cggttccttg
      301 aaatcgagga tgtggttccc catcgacaga tcgatcccga gatcgctcag cgtcagcccg
      361 ccaagatcga tgcgcgactc tgccatgccg acgcgcagcg acaggtcgat cggcacgccg
      421 gctggaatga gcactgtgag gcgcggccgg ttgggtcctt caccgcccat gcctccgagg
      481 atcctcgccc acaacggcgc cttcgacttg aaacggatcg tcgtccgcga atggccggcc
      541 tcgtcggcgg cggtgttttc ggtcagctcg tacatcccct cggcataggt gccttcgacc
      601 tggacctgcc ctccagccgg gcccttccga atggtgaagt ccccttccgc gagttcgacc
      661 gtcaaccgga gggggtcgcc gctcttcgag ccggcgccct tggcggcggc gccgctcgcg
      721 ccgccggcct gggccggatc tccagcgctc ggcggaccga gcgtgacggt cctggtgtga
      781 ttggcggggg taggatcgcc gagctccgca acggccgatc tggcccagct cacggcgacc
      841 gccacgccga cagcgacgac cacgacgatc gtgaagcacc cgattccggc aacgaggccg
      901 gccatctgcc agccgctcag tttcttcttc atcgcgttgc tggacctctc tcgttaggcc
      961 gccaggggct gcgcccttga agtcgagcgt caacgaccag atacgtcgcg ggcggcattg
     1021 tagttcgctt tcaacctttg cccttgccct ttgatagccg cccgttcggg tgatggcagc
     1081 ggcgatgccg tctgtcagaa tcggtttcat gttgaacgaa ctttcgaatc atgtcgcgga
     1141 tgtcgttgcg gccgtggcgc cgtcggtcgt gcaggtccat ggccgcaggc ggccggccag
     1201 cggcctcgtt tacgccgacg ggctcatcct gacgaccgtg cgcgcgattg gcggcgagga
     1261 cggcatcagg gtgaggcagc acgacgacgt ggacgtcgag gccgagcttg ccggctggga
     1321 cgcggcgacc agcctggcgc tcgtgcgcgt gtctggcgtg accgctccgc cgctccggcc
     1381 ggcgtcgtcg cccccgcgcg tcggcaacct ggccatcgcc gtggcgcgat cgtggagcaa
     1441 ctcggtcacg gccagcgcag gcatcgtgtc ggtgatcggc ggtccgtttc gcacgggcag
     1501 gcggcgggca atcgaccaga tcatccgcac gacggcgccg atgcacgacg gcttttcagg
     1561 aggcgcgttc atcgactcga ccggcgcgct catcggcgtc accacggcag cggcgattcg
     1621 cggcctgggc gtggtgattc cagcgggaat cgcctgggcg acggcggacc gcctcgcgca
     1681 gcacggacat gcccgccgtg ggtatctcgg cgtgctgggc cagcctgtcg cgttgtcggc
     1741 gtctcaatcc gcggcggccg gccgtgacaa ggccatgctc gtcgttggcg tgtcgcacgg
     1801 gagcgcagcg gcgcaggccg gcgtgctcgt cggcgacatc ctgctggcgt tcgagggtac
     1861 gcccacctcg tcgccggaag acctcctcga cctgctcgca gacgcgcagg cgggccgcca
     1921 ggccacgctg cacgtgcttc gcggggataa gccggtggac gtgcgggtga ccgtcggcga
     1981 gcgtcccggt cggtgaatgc gcgcgatact cgtgggcccc ccggctgcgc gcgagcgctt
     2041 gcggttggag ctggagtctc gcgcgatcga cgtcatcggc gagttcgagt cggagatcga
     2101 cgcgcgggcc tcgggcttga gcgccgatct gatcgtgtcg gcggcggact cgatgccccg
     2161 cgagcgcggt aacggcgatg atgcgattca ggagccgctg acaccgcgag agatcgaagt
     2221 gctcgagctg ctggcggaag ggctgccgaa caagggcatc gccgcccgcc tcgggatcag
     2281 cgaccagacg gtgaagttcc acgtcgccgc gatcatgggc aagcttggcg cgtccaatcg
     2341 caccgatgcc gtgaggcgcg ccgtgcgccg aggtctggtc tccctgt
//
LOCUS       DBNGSH010000164         2771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3407505, whole genome shotgun sequence.
ACCESSION   DBNGSH010000164 DBNGSH010000000
VERSION     DBNGSH010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3407505"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1741
                     /locus_tag="AC16CY_03740"
     CDS             395..1741
                     /locus_tag="AC16CY_03740"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3352920.1"
                     /translation="MRFPPFQVRSLANGLQVVMVSHHEQPAVTMRMLVKAGAAQDPDG
                     KGGLAELAAKLLDQGTTTRSAEQIADQIDSIGGALGTGSGTDLTFINAVVMKDSFALA
                     MDLVSDVVRNPAFSAEEIDRQREQAVSSLRVSDEDPGYVASVVFDRLVYGFHPYGRPG
                     TGTSASLQRITQNDLRAFHRQYFAPNNTILAIVGDIQADETFAAVERVFGPWPRGEVP
                     AAKPVDPPAPTRRLIVVDKPDSVQTEIRVGQLAIPRQHPDYLAFDLAVKILGGEGANR
                     LHRILRSERGLTYGAEADAQAFKQAGDFVAETNTRTETTAETLRLTVAEIARLQAQRV
                     FERELADAQAYLTGSFPLTIETPNDIATSVINAVFYELPIDEVRTYRERVQAISPDDI
                     QRVARQYVRPDRLSMVLVGNARAFLPDLREAGFTDVEVIPLDQLDLSAATLKREKR"
     gene            1747..>2771
                     /gene="lpxB"
                     /locus_tag="AC16CY_03745"
     CDS             1747..>2771
                     /gene="lpxB"
                     /locus_tag="AC16CY_03745"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR3352921.1"
                     /translation="MISCGEASGDMYAGALATELRRRAPEAEIFGFGGRRLQAAGGHL
                     IGDYGPFAVTGLTEAVRVFPRSFAMLRRLIDAARELRPNAFVAIDFPDFNFKLMAAIR
                     RLGIPIIYYISPQLWAWRPGRMDTMKAYVDRVLVIFPFEEALYQQAGVAVQFVGHPLV
                     DMSHPGQERSAFLAERGLDPAAPTVALLPGSRRNELERIVPVVSAAVPIIRARVPNAQ
                     FVVACAPNLPDALFARLVNDQPDARLVLVRDRTDDVLAASDVVITASGTATIQCVLHE
                     RPMVVVYRVSPLTYRLGKPFIKVDTFAMPNLVAGRRIVPELIQDDFTPERTANEALAL
                     LTDPARHA"
BASE COUNT          428 a          960 c          923 g          460 t
ORIGIN      
        1 acatcacgac ggccgacggc gagttcgaca tcttcatgaa cgtgacgagg gccgacgtcc
       61 agcgcgtcgc caagacctac ttcacgccgg ccaaccgtgt cgtgctccac gtcctgccga
      121 agggagcttc caggtgactc tgcgtcaact cccaattccc gactcccaat tcccaacggc
      181 gcagcgcccg gtggcacagc gtgcgggaag tattcgcccc gccggtcgac ggatagcgtc
      241 tttggggatt gggcgttgga agttgggcgt tgggagttgg gtgttgggag ttgggtgttg
      301 ggtgttggga gttggtctgg gcgtcctccc agcctccgcc cagtccgtag actggccgac
      361 tgagcggccc ccgcgtccgc tggcggcgcg cgaggtgagg ttcccgccct ttcaggtgcg
      421 gtcgctcgcc aacgggctgc aggtcgtgat ggtctcgcac cacgagcagc cggccgtgac
      481 aatgcggatg ctcgtgaagg cgggcgccgc gcaggacccg gacggcaagg gcgggctcgc
      541 cgagctggcc gcgaagctgc tcgaccaggg cacgacgacc cggagcgcgg aacagatcgc
      601 cgatcagatc gattcgattg ggggcgcgct ggggacggga tcaggcacgg acctcacctt
      661 catcaatgcc gtggtgatga aggactcctt tgcgctcgcg atggacctcg tgtccgacgt
      721 cgtgcgcaac cccgcattct cggccgagga gatcgaccgc cagcgagagc aggcggtctc
      781 gtcgctgcgc gtcagcgacg aagaccccgg ctacgtggcg tccgtcgtgt tcgatcggct
      841 cgtgtacggc tttcacccct acgggcgtcc gggcaccggc acgtctgcgt cgctgcagcg
      901 catcacgcag aacgacctgc gcgcgtttca ccggcagtac ttcgcgccca acaacacgat
      961 tctggccatc gtgggcgaca tccaggccga cgaaaccttc gcggcggtcg agcgcgtgtt
     1021 cggcccctgg ccccgcggcg aggtgccggc cgccaagccc gtcgatccgc ccgcgccgac
     1081 acgtcgcctc atcgtcgtcg acaagcccga ctccgtccag acggaaatcc gcgtggggca
     1141 gctggcgatc ccgcgccagc accctgacta cctcgccttc gacctcgcgg tgaagatcct
     1201 cggcggcgaa ggggcgaatc ggctgcaccg catcctgcgg tccgagcgcg gtctgaccta
     1261 cggggccgaa gcggacgcgc aggcgttcaa gcaggcgggc gacttcgtcg ccgagaccaa
     1321 cacccgcacc gagaccaccg ccgagacgct gcggctcacc gtcgcggaga tcgcacgcct
     1381 ccaggcccag cgcgtgttcg agcgcgagct ggccgacgcg caggcgtacc tgaccggcag
     1441 cttcccgctg acgatcgaga caccgaacga catcgcgacg agcgtcatca acgcggtgtt
     1501 ctacgaactg ccgatcgacg aggtgcgcac gtatcgggag cgcgtgcagg cgatttcccc
     1561 cgacgacatt cagcgggtgg cgcgccagta cgtccgtccc gatcgcctct cgatggtgct
     1621 cgtcggcaac gcgcgggcgt ttctccccga ccttcgcgaa gcgggcttca ccgatgtcga
     1681 ggtgattccg ctcgaccaac tcgatctctc cgcggcgacg ctgaagaggg aaaagcgctg
     1741 agaatcatga tttcctgcgg cgaagcgtcg ggcgacatgt acgccggcgc cctcgccacg
     1801 gagctgcgcc ggcgcgcgcc tgaggcggag atcttcggct tcggcgggcg gcgtctgcag
     1861 gccgcgggcg gacatctgat cggcgactac gggcctttcg ccgtcacggg cctgaccgag
     1921 gccgtccgcg tcttcccgcg atcgtttgcc atgctgcgcc ggctgatcga cgcggcccgg
     1981 gagctccggc cgaacgcctt cgtcgccatc gactttcccg acttcaactt caagctgatg
     2041 gccgcgatcc gccggctcgg cattcccatc atctattaca tcagcccgca gctctgggcg
     2101 tggcggccgg gccggatgga cacgatgaag gcgtacgtgg accgggtcct cgtgatcttt
     2161 ccattcgagg aggcgctgta ccagcaggcg ggtgttgccg tgcagtttgt cgggcatccg
     2221 ctcgtggaca tgagccatcc cggccaggag cgctcggcgt tcctcgccga gcgcggcctc
     2281 gatccggccg cgcccacggt ggccctgctg cctggaagcc gcaggaacga gctggagcgc
     2341 atcgtgcccg tcgtgagcgc cgccgttccc atcattcgcg cccgcgtgcc gaatgcgcag
     2401 ttcgtggtcg cctgcgcgcc gaacctgccc gacgccctgt tcgcgcgcct ggtgaacgat
     2461 cagcccgacg ccaggctcgt cctcgttcgc gatcggaccg acgacgtgct ggccgcgagc
     2521 gacgtcgtga tcacggcgtc cgggacggcc accatccagt gcgtactgca cgagcggccg
     2581 atggtcgtgg tctaccgcgt gtcgcccttg acctaccggc tcggcaagcc tttcatcaag
     2641 gttgacacct tcgccatgcc gaatctcgtg gccgggcgtc gcatcgttcc cgagctgatt
     2701 caggacgact tcacccccga gagaacagcg aacgaggcgc tggcgctgtt gaccgatccg
     2761 gcacggcacg c
//
LOCUS       DBNGSH010000165         1856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_351065, whole genome shotgun sequence.
ACCESSION   DBNGSH010000165 DBNGSH010000000
VERSION     DBNGSH010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_351065"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="AC16CY_03750"
     CDS             complement(<1..196)
                     /locus_tag="AC16CY_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3352922.1"
                     /translation="MSARNVFRLLVPAVVVLLVGAAAYAQATQTGAKPFEPTVGQAGK
                     DVVWVPTPQATVNRMLDMAKV"
     gene            352..930
                     /locus_tag="AC16CY_03755"
     CDS             352..930
                     /locus_tag="AC16CY_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR3352923.1"
                     /translation="MPANEVVDYYEILQISPNAEPETIHRVFRLLAQRYHPDNAETGN
                     EAHFRKLSEAYQVLSDPEKRARYDVAHTSQRQERWRLVSSGAQADNNFEAEQTVRLTV
                     LEVLYTRRRLELDSPGLSPLDLETLIGRAREHLEFTIWYLVQKKFITRSDGSLLQITA
                     EGVEYLEQNYRTNPQRRLGPGVAPKDARPIAS"
     gene            complement(1018..>1856)
                     /locus_tag="AC16CY_03760"
     CDS             complement(1018..>1856)
                     /locus_tag="AC16CY_03760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HIR3352924.1"
                     /translation="ASLAAQATAPSGKSFLWKIQAGSNVVYLAGSVHALNADAYPLSA
                     AFERAFNESGTLVEEIDLAEAGSPTLAPVLLAKGMYLDGRRFDGSVSKETAALVAGHL
                     KQSGLPAEMIQAMKPWMVMLTVAALEVQKAGLDPNLGLDRHFFTRAKNTGKAIVGLET
                     AESQIDRLDRMPDALQEQLLRSAIEDLELQRNEWKTLMSAWRGGDAATMEKMLLSSFK
                     PYPAAYQSLIVERNRNWMPLIDACFGKPRPCLVVVGAAHLVGPDGLLTMMRGKGYRVE
                     QQ"
BASE COUNT          336 a          617 c          592 g          311 t
ORIGIN      
        1 tgaccttcgc catgtcgagc atgcgattga cagtggcctg cggcgtcggc acccagacga
       61 cgtccttgcc ggcctggccg acggttggtt caaacggctt ggccccggtc tgcgtggcct
      121 gcgcgtaagc ggcggcgccg accaggagta cgacgactgc cgggaccaga agacgaaaca
      181 cattgcgtgc ggacatggcg ctctccagga gaaggatgac tgtaatttgc gcccaatagc
      241 gtataccgac cgggcgcatt cctgcgcatg agcctcggcc cgccccagac tggccagaac
      301 ccgccggccg gccgataacc cctaaagcgc cgttttagga ggagatccag catgccggcc
      361 aacgaggtgg tcgattacta cgaaatcctg cagatcagcc cgaatgcaga gcctgagacc
      421 attcaccgcg tgttccggct gctggcgcag cgctaccatc ccgacaacgc agaaacgggc
      481 aacgaggcgc acttccgcaa gctgagcgag gcctatcagg ttctcagcga tccggagaaa
      541 cgggcccgct acgacgtcgc gcacacgagc cagcggcagg agcgatggcg cctggtgtcg
      601 agcggcgccc aggcggacaa caacttcgag gccgagcaga ccgtgcggct caccgtcctc
      661 gaagtcttgt acacgcgccg gcgcctcgag ctcgacagcc ccgggctctc gccgctcgat
      721 ctcgagacgc tgatcgggcg cgcacgcgag cacctggagt tcaccatctg gtacctggtt
      781 cagaagaagt tcatcacgag gtccgacggt tctctgctgc agattacggc ggagggcgtg
      841 gagtacctcg agcagaacta caggacgaac ccgcagcgcc gcctcggtcc gggggtggcg
      901 ccgaaagacg cgcgcccgat cgcgtcctga caactcccaa tctccaactc ccgactccca
      961 aagattcgcg ccgggtctgc gttgggaatt gggaattggg agttgacgct cttcagccta
     1021 ctgctgttcg acgcggtagc ctttgcctcg catcatcgtg agcagcccat cggggcccac
     1081 caggtgcgcc gcgccgacga cgaccaggca cggccgcggt ttgccgaagc aggcgtcgat
     1141 cagcggcatc cagttccggt tgcgctccac gatgagcgac tggtacgccg cggggtacgg
     1201 cttgaacgaa ctgaggagca tcttctccat cgtggccgca tccccgcccc gccaggcgga
     1261 catgagcgtc ttccattcgt tgcgctggag ctcgagatcc tcgatcgcgc ttcgaagcag
     1321 ctgctcctga agcgcgtccg gcatccggtc caggcggtcg atctgtgatt cggccgtttc
     1381 gagacccacg attgccttgc cggtattctt ggcgcgggtg aagaaatgcc tgtcgaggcc
     1441 gaggttggga tcgagtccgg ccttctgtac ttcgagggca gccacggtca acatcaccat
     1501 ccacggcttc atcgcctgga tcatctcggc cggcaggccg gactgcttca ggtgcccggc
     1561 gaccagcgcg gccgtctcct ttgagacgct gccgtcgaac cgccgcccgt cgaggtacat
     1621 ccccttggcg agcagcacag gcgccagcgt gggcgagccg gcttccgcca ggtcgatctc
     1681 ctcgaccagc gtccccgact cgttgaacgc gcgttcgaac gcggcgctga gcgggtacgc
     1741 gtcggcgttg agcgcatgaa cggagccggc cagatagacg acattcgagc cggcctggat
     1801 cttccagaga aaactcttgc cagacggggc cgtcgcctgc gcggcgaggc tggccg
//
LOCUS       DBNGSH010000166         2531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_181496, whole genome shotgun sequence.
ACCESSION   DBNGSH010000166 DBNGSH010000000
VERSION     DBNGSH010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_181496"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1888)
                     /locus_tag="AC16CY_03765"
     CDS             complement(<1..1888)
                     /locus_tag="AC16CY_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3352925.1"
                     /translation="MSEISRTALFGKLNSLAYKAIEGATVFCKLRGNPYVELVHWIQQ
                     ILQAPDSDLHRIARHFELDSSRLARDVTEALDRLPRGATSISDLSSHVENAVERGWVY
                     ATLMFGEAQVRTGHLMVGMLKTPSLRNALVAISRQFEAVKSDTLSDDFRRIVNGSPED
                     ALSSRDGSSLGTPGEASDAMAPAQMGKQEALKRFATDLTDRARKGQIDPVSGRDEEIR
                     QIVDILMRRRQNNPILTGEAGVGKTAVVEGFAVRLAKGDVPPPLQNVSLYTLDIGLLQ
                     AGASMKGEFENRLRQVIDEVQASPKPIILFVDEAHTLIGAGGAAGTGDAANLLKPALA
                     RGTLRTIAATTWAEYKKYIEKDPALTRRFQVVQIGEPGEEKAILMVRGVASILEKHHR
                     AQVLDEALEAAVRLSHRYIPARQLPDKAVSLLDTACARVAISQHAVPAQLEDCRRTIE
                     ALQTELEIIGREESVGVDVGERRTSAEEKLAASEARRVELEARWQAEKGVVDRILEIR
                     AKLRKGREKVEGTGSTLEAAAQAEKTTAPTGAEALKPEERQAMLAELSDLQAKLKGLQ
                     GESPLILPSVDEHAVASVVQDWTGVPVGRMVKNEIEAVLNLAETLNQRVIGQRHALEM
                     IAKRI"
     gene            complement(1885..>2531)
                     /gene="tssG"
                     /locus_tag="AC16CY_03770"
     CDS             complement(1885..>2531)
                     /gene="tssG"
                     /locus_tag="AC16CY_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HIR3352926.1"
                     /translation="RAWAQAQPHVSHDRPGVDRFMVYVGSFLGMAPNEARDRDATPDL
                     AKLFHVGALIRQVRNAEGLRRILQHFFKVPVGIEEFVGHWLPLGARERTYLGHDGATL
                     GAGSVLGAGVWDRQHKFRIRLGPLTLKQYESFLPGGTPLRELVDWIRLYLCFELDWDV
                     RLMLKEREVPPLTLGGGGRLGWTTWLGQRRRGTDADDVCLDAEAFVDREGVRAA"
BASE COUNT          358 a          921 c          807 g          445 t
ORIGIN      
        1 ggatgcgctt ggcgatcatc tcgagcgcat gacgctggcc gatcacgcgc tggttgagcg
       61 tctcggccag gttcagcacg gcctcgatct cgttcttcac catgcggccg accggcacgc
      121 ccgtccaatc ctgcacgacg gacgccaccg catgttcgtc cacgctcggg aggatcagcg
      181 gcgactcgcc ctgcaacccc ttcagcttcg cctgcaggtc cgacagctcg gcgagcatcg
      241 cctgccgctc ttccggcttc agcgcttcgg ccccggttgg cgcggtggtc ttctccgcct
      301 gcgccgccgc ctcgagcgtg ctgcccgtgc cttcgacctt ctcgcggccc ttgcgaagtt
      361 tcgcccggat ctcgagaatg cggtccacga cgcctttctc ggcctgccag cgcgcttcaa
      421 gctcgacgcg gcgggcctcg ctcgcagcca gcttctcttc cgccgatgtc cggcgctccc
      481 cgacatcgac tcccactgat tcctcgcggc cgatgatctc gagctcggtc tgcagcgcct
      541 cgatcgtcct gcggcagtcc tcgagctgcg cggggaccgc gtgctggctg atcgccacgc
      601 gcgcgcacgc ggtatcgagc aggctgacgg ccttgtccgg cagctgacgc gcgggaatgt
      661 agcgatgcga cagccgcacc gccgcttcga gcgcctcgtc cagcacctgg gcgcgatggt
      721 gcttctcgag gatcgacgcg accccgcgga ccatcaggat ggccttctct tcgcccggct
      781 ccccgatctg cacgacctgg aaccggcggg tcagggcggg atccttctcg atatatttct
      841 tgtattcggc ccaggtggtc gcggcaatcg tgcgcagcgt gccgcgcgcc agcgccggtt
      901 tcagcaggtt tgcggcgtct cccgttccgg cggctccacc cgcgccgatg agcgtgtgcg
      961 cctcgtccac gaacaggatg atcggctttg gcgaggcctg gacttcgtcg atcacctgcc
     1021 gcagccggtt ctcgaactcc cccttcatgc tcgcgcccgc ctgcagcagg ccgatgtcga
     1081 gcgtgtacaa cgacacgttc tgcagcggcg gcggcacgtc gcccttcgcc aggcgcaccg
     1141 cgaacccctc gacgacggcg gtctttccca cgccggcctc gccggtgagg atcggattgt
     1201 tctggcggcg gcgcatcagg atgtcgacga tctgcctgat ctcctcgtcg cgtccgctca
     1261 ccgggtcgat ctgccccttg cgcgcgcggt cggtgagatc ggtggcgaat cgcttgagcg
     1321 cctcctgctt ccccatctgc gcgggcgcca tcgcgtcgct cgcctctccc ggggttccga
     1381 gcgacgatcc gtcgcgggag ctgagcgcat cctctggcga gccgttgacg atgcggcgga
     1441 aatcgtccga cagcgtgtcg ctcttgacgg cctcgaactg gcgcgagatc gccacgagcg
     1501 cgttccgcag cgacggcgtc ttcagcatcc cgaccatgag atggcccgtg cggacctgcg
     1561 cctcgccgaa catcagcgtg gcgtataccc agccgcgctc caccgcgttc tcgacgtgcg
     1621 acgagaggtc cgagatggac gtcgctccgc gcgggagccg atcgagcgcc tcggtgacgt
     1681 ccctggcgag ccgcgacgaa tcgagctcga agtggcgggc gatgcggtgc aggtccgagt
     1741 cgggcgcctg caggatctgc tggatccagt gcacgagctc cacgtacgga ttgccgcgca
     1801 gcttgcagaa caccgtcgcc ccttcgatgg ccttgtaggc gaggctgttc agctttccga
     1861 acagcgccgt tcggctgatc tcgctcatgc agctcgcact ccttctcgat ccacgaacgc
     1921 ctccgcatcc agacagacgt cgtcggcgtc ggtccctcgt cggcgctgac ccagccaggt
     1981 cgtccatccg agccgcccgc cgccgccgag cgtcaggggc ggcacctcgc gctccttcaa
     2041 catcagccgc acgtcccagt cgagctcgaa gcagagatac agacggatcc agtccaccag
     2101 ctctcggagc ggcgtgccgc cggggagaaa actctcgtac tgtttcagcg tcagcggtcc
     2161 gagacggatc cggaacttgt gctggcgatc ccagacgcca gcgcccagca cggagccggc
     2221 gccgagcgtc gccccgtcgt gcccgagata cgtgcgttcg cgcgcgccga gaggcagcca
     2281 gtgcccgacg aactcctcga tccccaccgg taccttgaag aagtgctgga ggatgcggcg
     2341 caggccttca gcgttccgca cctgccggat gagcgccccg acgtgaaaca gcttggccag
     2401 gtcgggcgtg gcatcgcggt cgcgagcctc gttcggcgcc atcccgagga aggacccgac
     2461 gtacaccatg aagcgatcga cgcccggccg atcgtggctg acgtggggct gcgcctgggc
     2521 ccacgcgcga t
//
LOCUS       DBNGSH010000167         2172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1030353, whole genome shotgun sequence.
ACCESSION   DBNGSH010000167 DBNGSH010000000
VERSION     DBNGSH010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1030353"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="AC16CY_03775"
     CDS             complement(<1..1443)
                     /locus_tag="AC16CY_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352927.1"
                     /translation="MRPVLPLLGLLALVSANVPIDAQTGAGADSSTPAAGAVQSFSQV
                     RLASDLAAVQQFRPSYPFWQHIFTIPDGRIAFGSARDGRLLAIFPSAGNWIEDARWED
                     QTLAGALAGQSLPKRLNDRREYVVRLLEPTTGPLVHNPTRGQFLMPNAVRYGSFLEEW
                     GAIYERFGVPAEVGLAQAILESGLNGTARSRARALGFCQWLSRNWSYLDRLSPDVLQA
                     YNQTTQAPYCAAYLSILATMYGSFIPALSEHHAGGVNVGRTVINGERLGGSDARAQYF
                     LGSEFAANLRDVSITRYKDLFRTYGPRSSLYAEMVFGNMINVRRLIAEHPQEPIFAMR
                     TTKALPLLDITARTGLKVDEVKRYNPALQGRVPARSNLYLPFYVQTFGEDVTFWHQPP
                     SPGFTTVLDDFLRIDAGVERWHEASFEATLRGFQARFEATKTEEGSVMAITLEYVIDD
                     LRTSRRAAILEDFRTSDHILDLFRKGVEELG"
     gene            1535..>2172
                     /locus_tag="AC16CY_03780"
     CDS             1535..>2172
                     /locus_tag="AC16CY_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352928.1"
                     /translation="MKPWVAGGLVAVACIAVVRPLRAQWPAHPDPGIPRTADGRPNLE
                     APTPRTPDGKPDLSGVWLNEWFYGGQVRQPPASPAGEPPPSAFADVGANMPGGLPFQP
                     WAKELMARRKEQNSKDNPDAHCLPMGLMQFHEHPQPRKMVQTPAVLLILYEGNFGVRQ
                     ILTDGRPLPDNDPQPWWYGYSVGTWEGDTLVVQTKGLRDGGWLDIYGSPLTDA"
BASE COUNT          379 a          710 c          742 g          341 t
ORIGIN      
        1 gcccagctcc tcgacgccct ttcgaaacag gtcgaggatg tggtcgctcg tccggaagtc
       61 ttcgaggatg gctgcccggc ggctggtgcg caggtcgtcg atcacgtact cgagcgtgat
      121 ggccatcacg cttccttctt cggtcttcgt ggcctcgaag cgtgcctgaa aacctctgag
      181 cgtcgcctcg aacgaggcct cgtgccaccg ctcgacgccc gcgtcgatcc tgaggaagtc
      241 gtcgaggacc gtcgtgaacc cgggcgacgg cggctgatgc cagaacgtga cgtcctcgcc
      301 gaaagtctgc acgtagaacg gcaggtacag gttcgatcgc gctggaacgc ggccctgcag
      361 ggcggggttg taccgcttga cctcgtccac cttgagcccg gtccgcgccg tgatgtcgag
      421 aagcggaaga gccttcgtcg tccgcatcgc gaagatcggc tcctgcggat gctcggcgat
      481 cagcctccgg acgttgatca tgttgccgaa caccatctcg gcgtacaagg acgaacgtgg
      541 accgtacgtc ctgaacagat ccttgtaacg cgtgatcgac acgtcacgaa ggttggcggc
      601 gaactccgat cccaggaagt actgcgcgcg cgcgtcggac ccgccgagcc gttcgccgtt
      661 gatgaccgtc cggccgacgt tgacgccgcc ggcgtgatgc tccgacagag ccgggatgaa
      721 gctgccgtac atcgtggcga ggatcgacag atacgccgcg cagtacggcg cctgcgtcgt
      781 ctggttgtac gcctggagga cgtcgggcga cagacgatcg aggtagctcc agttccggct
      841 gagccactgg cagaagccga gggccctcgc gcgcgaccgc gcggtcccgt tcaggccgga
      901 ctcgaggatg gcctgagcca ggccgacctc cgcggggacg ccgaaccgtt cgtagatcgc
      961 gccccactcc tcgaggaagc tgccgtagcg gaccgcgttc ggcatgagga actgcccgcg
     1021 cgtcgggttg tgcacgagcg gaccggtcgt cggctcgagc agccggacga cgtactcgcg
     1081 ccgatcgttg aggcgcttgg gcagcgactg gccggcgagc gcaccggcga gcgtctggtc
     1141 ttcccacctg gcatcctcga tccagttgcc agcgctcggg aagatcgcca gaagacgtcc
     1201 gtcgcgcgcg ctcccgaacg cgatgcgccc gtcaggaatc gtgaagatgt gctgccagaa
     1261 cgggtaggac gggcgaaact gctgcacggc cgccagatcg gaagcgagcc ggacctgaga
     1321 gaaactctgg acggccccgg cggccggtgt tgacgaatcc gccccggcgc cggtctgcgc
     1381 atcgatggga acgttcgcac tgacgagggc aagcaatccg aggagcggga gtacaggtct
     1441 catcgtggtt cgggcacggc caatccagcc ggagcgattt acggtccact agatcttatc
     1501 gacaaatggg gccggaacgt tcaggatcgt ggcgatgaaa ccctgggtgg caggcggtct
     1561 cgtggcggta gcgtgcatcg ccgtcgtgag acccctgcgg gcgcagtggc ccgcgcatcc
     1621 ggacccgggc atcccgagga cggcggacgg ccggccgaat ctcgaggccc ccacgccccg
     1681 gaccccggac gggaaaccgg atctcagcgg cgtgtggctg aacgagtggt tctacggcgg
     1741 gcaggtcagg cagcctccgg catcgccggc gggcgagccg ccgccgtccg cgtttgccga
     1801 cgtcggcgcc aacatgcccg gcggtctccc gttccagccc tgggcgaagg agttgatggc
     1861 ccgtcgcaag gaacagaaca gcaaggacaa cccggacgcg cattgcctgc ccatgggcct
     1921 catgcagttt cacgagcacc cgcagccgag gaagatggtc cagacacccg ccgtgctgct
     1981 gatcctgtac gagggaaact tcggtgtcag gcagatcctg accgatggcc gtccccttcc
     2041 cgacaacgac ccgcagccgt ggtggtacgg ctactcggtc ggcacgtggg agggcgacac
     2101 gctcgtcgtc cagacgaaag gccttcggga cggcggatgg ctcgacatct acggcagccc
     2161 gctcaccgac gc
//
LOCUS       DBNGSH010000168         4049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_861236, whole genome shotgun sequence.
ACCESSION   DBNGSH010000168 DBNGSH010000000
VERSION     DBNGSH010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_861236"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="AC16CY_03785"
     CDS             complement(<1..1130)
                     /locus_tag="AC16CY_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR3352929.1"
                     /translation="MKILSMRRVLRALVALGVVLVVAALALGLWARGRLRASLPLLAG
                     TLRVEGLSAQVTVTRDALGIPTIAGASREDVALATGFLHAQDRFFQMDLARRRAAGEL
                     SALLGGRAFEADLQVRVHRFRAEAERALEKMDARWRVILDAYTAGVNSGLAQLGAPPF
                     EYLLLRRQPAPWRTEDSLLVVLSMFITLQDPDGAYEATLATMHDVLPEAMAYFLAPIG
                     TEWDSPVVGEAFSVPPIPGPDVYDLRARMSGERRYRIRPGPERGRVPIPDLGRIQLPW
                     DLGVERGEAVLGSNNWAVAGRLTTGGGALLANDMHLQVRVPNTWYRAVLEWPDPADPS
                     SPHRLIGITLPGIPSLVVGSNTYVAWGFTNSQGDWSDLVLLDV"
     gene            1158..2378
                     /locus_tag="AC16CY_03790"
     CDS             1158..2378
                     /locus_tag="AC16CY_03790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3352930.1"
                     /translation="MTRTRYGLSPWFETFPRSRRPGHPRLRGELSSDVAIIGGGLTGC
                     ATAYACAAAGLNSIVLEAGSLGQGGTARGAGLLLPEPGPDFRTLAKLHGLRAARRMFE
                     LWRRASLDGGALIRRLGISCGLEPGGSLTVASWDGEKALRQECDARTQAGLDAQWVNA
                     ASISRTSALEAAAGVRLGGCFTFDPYRACLGLASSAAKRGVRFFERSPVKKVRVGSKR
                     VEVVAEGGLVRAATVIVCTGTATSEYHPLRRHFKRREKYLVMTEPVPAAIRKQLGSQG
                     LTVGDTASPPHRIRWTRDNRLLVTGADQAETPARKRHAVLVQRTGQLMYELLRMYPAI
                     SGLQPEHGWECVYGETADGLIYIGPHRNYPRHLFALGGGADSMTGAFLASRLLARAAS
                     GTPEKGDDVFGWTR"
     gene            2375..3088
                     /gene="bshB1"
                     /locus_tag="AC16CY_03795"
     CDS             2375..3088
                     /gene="bshB1"
                     /locus_tag="AC16CY_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HIR3352931.1"
                     /translation="MTSAVTAVDLLAIGPHPDDIEIGIGGVVAKHVSLGHRVGLCDLT
                     AGEMGSNGTVDERLGEAEAARKVLGAAWRVNLRVPDRAIGSDQEHTRIVAGLIRRARP
                     RVVAVPYWSDRHPDHEAASRLLVEGVFNAGLRRYAAEGDAWKPESICYYFINDSTTPS
                     FVVDVSDQYETKRRALACFASQFRPAQPDAVPTRLTAATFTRLIESRDAQFGAQAGVA
                     FAEGLVVKQPLLRPHLLPE"
     gene            3085..>4049
                     /gene="bshA"
                     /locus_tag="AC16CY_03800"
     CDS             3085..>4049
                     /gene="bshA"
                     /locus_tag="AC16CY_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HIR3352932.1"
                     /translation="MTRPLNVGIVCYASVGGSGIVATELGKALALRGHQAHFISTETP
                     FRLGDFQEGLFFHQVLTPSYPLFREPQYLLSLTNRVVQVARESRLDIIHAHYAVPHAT
                     AAFVARQILAAEKGAAAPRVVTTLHGTDITLVGSDPSYSHIVAFSIEQSDAVTAVSTS
                     LRESTYAELGVTHPIAVIPNFLDCAVHRRRARPELRRRFSGGNEATKIVAHVSNFRPV
                     KRVDAVVEIFDRIRKQVPARLLLVGDGPDLPMAQQMARDLGIGGLVEAVGAQEEVVPL
                     LSVADLFLLPSSQESFGLAALEAMACEVPVIASRVGGLPEVIEHGV"
BASE COUNT          630 a         1390 c         1406 g          623 t
ORIGIN      
        1 acgtcgagca gcacgaggtc gctccagtcg ccctgcgagt tggtgaagcc ccacgccacg
       61 tatgtgttgc tgccgacgac gagcgagggg atgcccggaa gcgtgatgcc gatcagcctg
      121 tgcggcgacg agggatcggc cggatcgggc cattcgagca cggcccggta ccacgtgttc
      181 ggcacgcgta cctggaggtg catgtcgttg gcgaggagcg cgccaccgcc ggtcgtgagg
      241 cggccagcga ccgcccagtt gttgcttccc agcacggctt cgcctcgctc cactcccaga
      301 tcccaaggaa gctggatgcg gcccaggtcc ggtatcggaa cacgaccgcg ctccggcccc
      361 ggcctgatgc gatagcgacg ctcgcccgac atccgggcgc gcaggtcgta cacatccggg
      421 cccggaatgg gcggcaccga aaacgcctcg ccgacaacgg gcgagtccca ctcggtgccg
      481 atcggggcga ggaagtacgc catcgcctcg ggcagcacat cgtgcatcgt cgcgagcgtc
      541 gcttcgtagg cgccgtccgg atcctgcagc gtgatgaaca tcgagaggac gacgagcagc
      601 gagtcctcgg tgcgccatgg cgcgggttgc cgccgaagca gcaggtattc gaacggcggg
      661 gcaccgagtt gcgcgaggcc gctgttgacg cccgcggtgt acgcatcgag aatcacgcgc
      721 caccgggcat ccatcttttc gagcgcgcgc tccgcttcgg cgcgaaagcg gtggacgcgg
      781 acctggagat ccgcttcgaa cgctcgccct cccaggagcg ccgacagctc gcccgcggcg
      841 cgccggcgcg cgagatccat ctggaagaac cggtcctgcg cgtggaggaa gcccgtggcg
      901 agcgcgacgt cttcgcgcga agcaccggcg atcgtcggaa tgccgagcgc gtcgcgcgtc
      961 accgtgacct gcgccgagag gccttcgacc cgcagcgtgc cggcgagcag cggaaggctg
     1021 gcccgaagcc ggcctcgcgc ccagagaccg agcgccaggg cggccaccac cagcacgacc
     1081 cccaaagcca cgagcgcgcg gagtacccgg cgcattgaga ggatcttcat caatgccttg
     1141 taaaatgctg accggcgatg acacggaccc gttacggact ctctccctgg ttcgagacct
     1201 ttcctcgttc gcgccgcccg ggccatccac gtcttcgcgg cgaactctcc tcggacgtcg
     1261 ccatcatcgg cggcgggttg accggttgcg ccaccgctta cgcgtgcgcg gccgccgggt
     1321 tgaattcgat tgtgctcgaa gctggatccc tcgggcaagg gggcacggcc cgcggcgcgg
     1381 gactgttgct gccggagccc gggcccgact tcagaaccct tgcgaagctg cacgggctgc
     1441 gcgccgcccg gcggatgttc gagctctggc gccgcgcgtc gctcgacggg ggggcgctca
     1501 ttcgacgcct ggggatctcc tgcggcctcg aacccggcgg cagcctgacg gtggcgtcgt
     1561 gggacggcga gaaggcgctt cgccaggaat gcgacgcgcg cacgcaggcc gggctcgatg
     1621 ctcagtgggt caacgcggcg tccatcagca gaacctcggc gctcgaagcc gcggcgggag
     1681 tgcgtctcgg aggctgcttc acgttcgatc cctatcgtgc gtgcctcggc ctggcatctt
     1741 ccgccgcaaa gcggggcgtg cgcttcttcg agcgctcgcc ggtcaagaag gttcgcgtgg
     1801 ggagcaagcg ggtcgaggtc gtggccgagg gcggtctggt tcgcgccgca acggtgatcg
     1861 tgtgcacggg cacggccacc tccgagtacc acccgttgcg gcggcacttc aagcggcgcg
     1921 agaaatatct ggtcatgacc gagccggtcc ccgcggcgat ccgaaagcag ctgggtagcc
     1981 aggggctcac ggtcggggac accgcgtcgc cgccgcatcg catcaggtgg acgcgcgaca
     2041 atcggctgct cgtcaccggt gcggaccagg ccgagacgcc ggccaggaag cggcacgcgg
     2101 tgctcgttca acgcacgggt cagttgatgt acgagctgct ccggatgtac ccggcgatct
     2161 cgggcctgca gccggagcac ggctgggagt gcgtctacgg cgaaacggcg gacggcctga
     2221 tctacatcgg cccgcatcgc aactacccgc gccacctgtt tgccctcggc ggcggcgccg
     2281 actcgatgac gggcgccttt ctcgcctccc gcctgctggc tcgcgccgcg tcgggcacgc
     2341 cggagaaggg cgacgacgtg ttcggctgga cgcgatgacg agcgctgtca cggcggtgga
     2401 tcttcttgcg atcggacccc accccgacga catcgagatc ggcatcggcg gcgtcgtggc
     2461 caagcacgtg tcgctgggac accgcgtggg gctgtgcgac ctgactgcgg gagagatggg
     2521 aagcaacggc acggttgacg agagactcgg cgaagcggag gccgcgcgca aggtgcttgg
     2581 cgccgcatgg cgcgtcaacc ttcgagtgcc ggaccgcgcc atcgggagcg accaggagca
     2641 cacacggatc gtggccgggc tcatccggcg tgcccggccg cgcgtggtcg cggtcccata
     2701 ctggtccgat cgccatcccg atcacgaagc ggccagccgc ctgctcgtcg agggcgtgtt
     2761 caacgccggg ctgcgccgct acgccgccga gggcgacgcc tggaagccgg agtcgatctg
     2821 ctattacttc atcaacgact caacgacgcc gtcgttcgtg gtggacgtga gcgaccagta
     2881 cgagaccaag cggcgggcgc tggcatgctt cgcgagccag ttccgtccgg ctcagcccga
     2941 tgcggtgccg acgcgcctga ccgcggccac cttcacgcgg ctgatcgaga gccgcgacgc
     3001 gcagttcggc gcccaggcgg gcgtcgcctt cgccgaaggc ctcgtcgtca aacaacccct
     3061 tctccggccg cacctgctac ccgagtgacc cggcccctca acgtcggcat cgtctgttac
     3121 gcctcggtcg gtggaagcgg aatcgtcgct accgaactgg ggaaggcgct cgccctgcgc
     3181 gggcaccagg cgcacttcat cagcaccgag acgcccttca ggctcgggga cttccaggag
     3241 ggcctgttct tccatcaggt cctgacgccg agctacccgc tgttccgcga gccgcagtat
     3301 ctgctgtcgc tgacgaaccg cgtcgtgcag gtcgcgcgcg agtcgaggct cgacatcatt
     3361 cacgcgcact acgcggtgcc gcacgcgacg gccgctttcg tggccaggca gatcctcgcc
     3421 gcggaaaagg gagccgccgc ccctcgcgtg gtgacgacgc tgcacggcac ggacatcacg
     3481 ctcgtcggca gcgatccgtc gtattcgcac atcgtcgcgt tctccatcga gcagtcggac
     3541 gcggtgaccg ctgtctcgac cagccttcgc gagtcgacct acgccgagct cggcgtcacg
     3601 catccgatcg cggtcattcc caactttctc gactgcgcgg tccatcgacg ccgcgcgagg
     3661 ccggagttga ggcgacggtt ctccggcgga aacgaggcga ccaagatcgt ggcccacgtc
     3721 tcgaacttcc gccccgtcaa gcgtgtggat gccgtcgtcg aaatcttcga ccgcatcagg
     3781 aagcaggtgc cggcgcggct cctgctcgtc ggcgatggcc ccgatctgcc gatggcccag
     3841 cagatggcga gagacctggg gattggaggc ctggtcgagg ccgtcggcgc gcaggaggaa
     3901 gtggtgccgc ttctctcggt cgccgacttg ttcctgctgc cgtcctcgca ggagagcttc
     3961 ggcctcgccg ccctcgaggc gatggcgtgc gaggtgccgg tcatcgcgtc gagagtcggc
     4021 gggctgcccg aagtcatcga gcacggtgt
//
LOCUS       DBNGSH010000169         7225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2729377, whole genome shotgun sequence.
ACCESSION   DBNGSH010000169 DBNGSH010000000
VERSION     DBNGSH010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2729377"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..692
                     /locus_tag="AC16CY_03805"
     CDS             111..692
                     /locus_tag="AC16CY_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3352933.1"
                     /translation="MFRRRQQADADAFGAEVLGYLEPLYATALRLTRNRPDAEDLVQD
                     TLVKALRFSDRFERGTNLRAWLSTILHNTWRNRLRDASREAVEADSPRVEAAAEAGTV
                     AGSDSPEAILLRDTLDADLKAALDGLPEIFRQAVWLRDVEEFSYADMAKMLGVPIGTV
                     MSRISRGRRMLLKGLSTSRPAERGSAPSGTPTP"
     gene            689..1465
                     /locus_tag="AC16CY_03810"
     CDS             689..1465
                     /locus_tag="AC16CY_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3352934.1"
                     /translation="MNECRRVSECLAAYVDGLLPASERDSVDRHLSACSPCRRLAEDE
                     GTGQQILRERRAQLLVEPLPPGLRARCESVALAGRGPSPGLAIVGRWWRMRPVAVLAT
                     IVVMVFTASAFLSLMTQRSNALLAAQLTADHSRCFKLFAPRDGVSLDASDVERMLSEQ
                     HGWTVHVPPSSPAVGLELVGARRCMYGETAIPHVMYRAHGQDMSLFVLAGETRKAADL
                     VALDHRSSMWARDGTTFVLVLPDNESDLAEAIGYMRREAR"
     gene            1469..2089
                     /locus_tag="AC16CY_03815"
     CDS             1469..2089
                     /locus_tag="AC16CY_03815"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3352935.1"
                     /translation="MSMQDENNAVDGTPAPEAQEQQPSRKATWLAMGAVALAVGLIWM
                     APGLDTHDAETAGLEHPGEPEDAEAIGKPARLDFVLKDMHGADVDMQSYKGKIILLNF
                     WATWCGPCKAEIPSLVELQTQYADDLVVLGLSIDDPAEKLRPYAAEYKMNYPVLVGGR
                     EDVQEAFGPLFGVPVSVIIDREGKIAMKHSGMASKEQLERQIKTLL"
     gene            2102..2326
                     /locus_tag="AC16CY_03820"
     CDS             2102..2326
                     /locus_tag="AC16CY_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352936.1"
                     /translation="MLKGFTHARLACGCRVAFREGVEGSPVTVVVDEKAPGCLISLHV
                     RHLPLYDFREALRPPTRFLPLEEEEYEEEN"
     gene            2403..2744
                     /locus_tag="AC16CY_03825"
     CDS             2403..2744
                     /locus_tag="AC16CY_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3352937.1"
                     /translation="MAGERQKSDLLQGTLDVLILKVVALGPIHGYAIAQRLQQMSGAV
                     LQVQQGSLYPALHRLEKRRWVRAAWAASETGRDARFYTLTPLGRKQLEEQRENWDRLS
                     AAISGVLRTAE"
     gene            2748..5369
                     /locus_tag="AC16CY_03830"
     CDS             2748..5369
                     /locus_tag="AC16CY_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3352938.1"
                     /translation="MRFGQRRDPRVADEIRFHRERLIEQYIAEGMDRRAAERRAFLEF
                     GNPAQIEEEVRDVRGRWLTDLAQDARYALRTLRRNPVFAAVAVLSFALGIGANTTIFS
                     LVNAVILRTLPVEQADRLVQLGRMSTTTGRASSVSYPVFEHLRDNMTSVSSLFAHATT
                     AQSIFFGDDDDSVTTDFVSGHYFVVLGLVPAAGRLLAPSDDVPAPSVLAAVITDRYWQ
                     RRFGRSPSVVGTTFTLRDRVFTIVGVTPPAYTGAQSGRASDLMVPLVPMMTEQQRASI
                     GSNTLGVLARLKPGVTVAQASAEVDVLFRAFLQERSAALPDPAQRAAVLRQRAAAFPA
                     AGGFNPLRDNFAKPLLILMGIVGLILLLACVNLSGLLLARAAARQREISIRLAIGAGR
                     GRLVRQFLTESLVLALLGGGVGLLMTGRFSQRLFELVTAGREVQLSVAPDWRVIAFTA
                     ALALTACALAGLAPALQAARAGMNPALKDVAAHGHTRLGRTLVVSQFAMSMVLVVGAT
                     LFVGTLVKLYAVDPGFKSDGVLVLNVRSTRPLPPDRATAVGSALVERLSASAGVRAAS
                     AARVLPVEGNLWDRTIQVEGYAFRSGESNSVGFNAIAPGYFATMGTPLLSGRDFGDRD
                     TGTAPKVAIVNASFERYFFGDRSALGRRVTSVGATYEIVGVVRDAKYQNLRDEILKTM
                     YIPWAQGEDQGASYSYLARVAEGDPLRLVPGVERMVREVDPSLRVQRVSTYAAMIERS
                     ISTERVMATLGGLFGLLALVVAGLGMFGVLAFQVARRTNELGVRMVLGARRSSVMGLV
                     LRDVAAMIVPGIAIGAGVALMLTDIARGILFDFTPSEASVFVVAACALGLTALLAGWF
                     PALRASRVDPLIALRHE"
     gene            5392..5733
                     /locus_tag="AC16CY_03835"
     CDS             5392..5733
                     /locus_tag="AC16CY_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352939.1"
                     /translation="MNRFGFVVVAVVCVAVAITSHAQQGLSGKWQGTTAQGRSVGLEL
                     KVSGAELTGTFTRDQQSANIAEGQVDDKTFSFTVHVDGKAATFTGRLAGEAIELTIQG
                     SKNTVTLTRAK"
     gene            5747..6409
                     /locus_tag="AC16CY_03840"
     CDS             5747..6409
                     /locus_tag="AC16CY_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352940.1"
                     /translation="MGKRIFLLSPANCGGVRARRVVSDAAEFALARQLRTTEGAPLGD
                     VFSFVSGLYFRGKLAYARRFTRPPDPTDRVVADGVLVITPNAGLRAADVAVTRQSIRA
                     FAKVDIDLENPGYRGPLEQGARVLAASVGPDCDVVLLGSIASGKYVEVLLPIFGERLL
                     FPPAFIGRGDMSRGGLMLRCAESGLELDYAPVAGAVRHGQRPPRLPPLRQRDSAIRER
                     VR"
     gene            6435..6950
                     /locus_tag="AC16CY_03845"
     CDS             6435..6950
                     /locus_tag="AC16CY_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HIR3352941.1"
                     /translation="MSDRRREPDLAPPFEQLLRELLHWARDHRALAARVGLDIDDLVQ
                     DTVVRLARSGFEIRHAGAVRLYARASVMNRIRDTVRRAEEQQRNGGYTPYDDDDLSFQ
                     RDLELLQRLHPRDRRAILERLERPFDAGELRELYDLAGEGVLRQALRWLRQLFGRDRD
                     GEPPSGPLSPA"
BASE COUNT         1129 a         2317 c         2474 g         1305 t
ORIGIN      
        1 atactgctcc cgcctgctcg cccccgggac gccccgccaa tcgacgcgtt cgagcggttc
       61 gggaataacg gcgccggagc ccgggttgat cggttaggtg ccggcatccg atgttccgac
      121 gccgccagca agctgacgct gacgcgtttg gcgcggaggt cctcggttac ctcgagccct
      181 tgtacgcgac ggcgctgagg ctcaccagga accggcccga tgccgaagac ctcgtgcagg
      241 acacgctcgt caaggccctg cgcttttcgg accggttcga gcgcggaacg aacctgaggg
      301 cgtggctgtc cacgatcctc cacaacacgt ggaggaaccg gcttcgggat gcgagccggg
      361 aggccgtcga ggctgacagc cctcgggtgg aagcggcagc ggaggcaggc acggtcgcag
      421 ggtcggactc gccggaggcg attctgcttc gcgacacgct cgacgccgat ctcaaggcgg
      481 cgctcgacgg cctgcccgag atcttccgcc aggcggtgtg gcttcgcgac gtggaggagt
      541 tttcatacgc ggatatggca aagatgctcg gcgtgccaat cggaacggtc atgtcgcgca
      601 tttcgcgggg ccggagaatg ctcctgaagg ggctgagcac ctcgaggccc gcggagcggg
      661 ggagcgcgcc ctcgggaacg ccgacgccat gaacgagtgc cgtcgcgtct cagagtgtct
      721 ggccgcctac gtggacggcc tgcttcccgc gtccgagcgc gacagcgtcg atcgtcacct
      781 gtcggcctgc tccccatgcc gccggctggc agaggacgag ggtaccgggc agcagattct
      841 ccgcgagcgg agagcgcaac tgctcgtcga gccgctgccg ccgggactgc gtgcgcggtg
      901 tgaatcggtc gcgctggccg gtcgcgggcc gtcccctggt cttgccatcg tcgggcgctg
      961 gtggcgcatg cggcccgtcg ccgtcctggc gaccatcgtc gtgatggtgt tcacggcgtc
     1021 cgcgttcctc tccctgatga cgcagcggtc caatgccctg ctggccgcgc agctcaccgc
     1081 cgaccactcg aggtgcttca agctgtttgc gccgcgcgac ggtgtatctc tggacgcctc
     1141 ggacgtcgag cgcatgctgt cggagcagca cggctggact gtgcacgtcc cgccatcatc
     1201 gccggcagtc ggcctcgagc tcgtcggtgc caggcggtgc atgtacggag agacggcgat
     1261 tccccacgtg atgtatcgcg cccacggtca ggacatgtcg ctgttcgtcc tggcgggaga
     1321 gaccaggaag gcggccgatc tggtcgctct cgatcaccgg tcgtcgatgt gggcgcggga
     1381 cgggacgacg ttcgtgctgg ttcttcccga caacgagagc gacctggcgg aagccatcgg
     1441 ctatatgagg cgtgaagcac gataaactat gagtatgcag gacgagaaca acgccgttga
     1501 cgggactccg gcgccggagg ctcaggaaca gcagccgtcg cgcaaggcga cgtggctggc
     1561 catgggggcg gtcgcgctgg ccgtcgggct catctggatg gctcccgggc ttgacacgca
     1621 cgacgcggag acggccggct tggagcatcc cggcgagccc gaggacgccg aagcgattgg
     1681 caagcccgcc cgcctcgact tcgtcctcaa ggacatgcac ggcgccgacg tggacatgca
     1741 aagctacaaa ggcaagatca tcctgctcaa cttctgggcg acgtggtgcg ggccctgcaa
     1801 ggccgagatt ccctcgctcg tcgaactgca gacgcaatac gccgacgatc tggtcgtgct
     1861 ggggctgtcg atcgacgatc ccgccgagaa actgcggccg tacgcggccg aatacaagat
     1921 gaactacccg gtgctcgtcg gcggacgcga ggacgtgcag gaggcgttcg gcccgctgtt
     1981 cggcgtcccc gtctcggtga tcatcgaccg cgaggggaag atcgccatga aacactcggg
     2041 catggcgagc aaggagcagc tggaacgaca gatcaagacg ctgctgtagt atgatccgtg
     2101 catgttgaaa ggcttcacgc acgcccggct tgcctgcggc tgccgcgtgg cgtttcgtga
     2161 aggcgtcgaa ggaagtcccg tcacggtcgt cgtcgacgag aaagctcccg gctgtctgat
     2221 ctccctgcac gttcgacacc tgcccttgta cgacttccgc gaagcactgc gcccgcccac
     2281 gcgcttcctt cctctcgagg aagaggagta cgaagaagag aactagcgcg gcccgccgcg
     2341 tgttcaggtc agtctgcgta acgccttgac attctacata tgcatgtgta gattgccttg
     2401 gtatggccgg cgagcgccag aagtccgatc tgctgcaagg cacgctcgac gtgctcatcc
     2461 tgaaggtcgt tgcgctcggc cccattcacg ggtacgccat cgcgcagcgc ctccagcaga
     2521 tgtcgggcgc cgtgctccag gtccagcaag ggtccctcta tccggcgctc caccgcctcg
     2581 agaagcggcg ctgggtccgt gccgcgtggg ccgcgtccga gaccggtcgc gacgcgaggt
     2641 tctacacgct cacgccgctc ggccgcaagc aactcgaaga gcagcgcgag aactgggacc
     2701 gtctgtccgc ggcaatctcg ggggttctgc gcacggcgga atgagccatg aggttcggcc
     2761 agaggcgtga cccccgcgtt gccgacgaaa tccgttttca tcgcgagcga ttgatcgagc
     2821 agtacatcgc cgaagggatg gaccgccgcg cggccgagcg gcgcgcgttc ctggagttcg
     2881 gcaacccggc gcagatcgaa gaagaggtcc gcgacgtgcg tggccgctgg ctgaccgatc
     2941 tggcccagga cgcgcgctac gcgctgcgaa ccctccgtcg gaatcctgtc tttgccgcgg
     3001 tcgcggtgct gtcctttgcg cttggcatcg gagccaatac gaccattttc agcctggtca
     3061 acgccgtgat tctgcggacc cttccggtcg agcaggcgga tcggctcgtt cagcttggcc
     3121 gtatgtccac gactaccggt cgcgcgagtt ccgtgtccta tccggtgttc gagcatttgc
     3181 gggacaacat gacgtcggtc tcgagcctct tcgcgcacgc cacaacggcc cagtcgatct
     3241 tcttcgggga cgacgacgat tcggtgacga ctgatttcgt ctctggtcac tacttcgtcg
     3301 tcctcgggct ggtgccggct gccggacgtt tgctggcacc gtccgacgac gtgccggcgc
     3361 cgtccgtttt ggcggccgtg atcacggacc gttattggca gcgacgcttt ggacgcagtc
     3421 cttccgtcgt cggcacgaca ttcacgctcc gcgaccgggt gttcacgatc gttggtgtaa
     3481 cgcccccggc gtacacaggt gcccagtccg ggcgcgcgtc cgatctgatg gtgccgctcg
     3541 tcccgatgat gaccgaacag cagcgagcga gcatcgggtc caataccctg ggcgtcctcg
     3601 cgcggctgaa gcccggggtg accgtcgccc aggcctctgc cgaagttgac gtgctcttca
     3661 gagccttcct gcaggagcgt tctgcggcgc tgccggatcc ggcacagcgc gcggcggttc
     3721 ttcgccagcg tgcggctgcg ttcccggcgg ctggcggctt caacccgctc cgggataact
     3781 tcgcgaagcc gttgctcatc ctgatgggca tcgtcggcct gatcctgctg ctggcctgtg
     3841 tgaacctgtc ggggctgctg ctcgcgcgcg cggccgcgag gcagcgcgag atttcgattc
     3901 gactcgcgat cggcgcggga cgcggtcgtc tggtgcgaca gttcctgacc gagagcctgg
     3961 ttctcgcact gcttggagga ggggttggcc tcctcatgac gggaaggttc agccagaggc
     4021 tgttcgagct cgtgacggcc ggccgcgagg tgcagttgtc ggtggcgccc gattggcggg
     4081 tcatcgcgtt cacggccgca ctcgcgctga cagcctgcgc gcttgccggt ctcgcccccg
     4141 cgcttcaggc ggcccgtgcg gggatgaacc ccgcgctcaa ggacgtggca gcgcacggac
     4201 acacccggct tgggcggacg ctcgtcgtgt cgcagttcgc gatgtcaatg gttctcgtcg
     4261 ttggcgccac gctgttcgtc ggcacgctcg tcaagctgta cgccgtcgac ccgggattca
     4321 agagcgatgg tgtgctggtg ctgaacgtca ggagcacccg cccgcttccg ccggatcgcg
     4381 cgacggccgt cggaagcgcg ctggtcgagc ggctgagtgc atccgcaggt gttcgagcgg
     4441 caagcgctgc ccgcgtcctg cccgtcgagg gcaacctctg ggatcggacc atacaggtcg
     4501 aggggtacgc gtttcgatcg ggggaatcga actcggtcgg cttcaacgcg attgcccccg
     4561 ggtacttcgc tacgatggga acaccactac tttcaggccg cgacttcggc gatcgcgaca
     4621 ccggcacggc gccaaaggtg gccatcgtca atgcaagctt cgagcggtac ttcttcggcg
     4681 accgatccgc gctcggccgt cgcgtgacct cggttggagc gacgtacgag atcgtcggcg
     4741 ttgtccgcga cgcgaaatac cagaatctgc gcgacgagat cctgaagacg atgtacatcc
     4801 cctgggcgca gggggaagac cagggagcga gctattccta cctggctcgc gtggcagaag
     4861 gcgacccgct gcgcctggtt ccgggtgtgg aacgcatggt ccgtgaggtc gatccctcgt
     4921 tgcgtgtgca gcgagtctcg acctacgcgg ccatgatcga gcgatccatc tccactgaac
     4981 gggtcatggc cacgctcggc ggtcttttcg gattgctcgc gctcgtggtc gccggcctcg
     5041 gcatgttcgg ggtgctggcg ttccaggttg cacgccggac gaacgagctc ggcgtgcgaa
     5101 tggtcctggg cgctcgcaga tcgtccgtga tgggcctcgt cctcagggac gtcgccgcga
     5161 tgatcgttcc cgggattgcg atcggggctg gcgttgcgct gatgctgact gacatcgccc
     5221 gggggatcct tttcgacttc acaccaagcg aggccagtgt gttcgttgtc gccgcctgcg
     5281 cgctcgggct gacggcactt ctggcgggat ggttccccgc gctgcgcgcg tcccgcgtcg
     5341 atccgctcat tgcgctcagg catgaatgac gtccatacag gggggcatcg tatgaatcga
     5401 ttcggtttcg tcgtggtcgc tgtcgtgtgt gtggccgtcg ccatcaccag tcacgcgcag
     5461 caggggctca gcggaaagtg gcaggggacg accgcgcagg ggcgatccgt ggggctggag
     5521 ctcaaggtga gcggcgccga actgaccggg acgtttaccc gggatcagca atcggcgaac
     5581 attgccgaag gacaagtgga cgacaagacg ttctcgttca ccgtacacgt ggacggcaag
     5641 gcggcgacct tcaccggacg gctggctggc gaggcgatcg agttgactat ccagggctcc
     5701 aagaacaccg tcacgctgac gcgtgcgaaa tagacgccgg tctccgatgg ggaagcgcat
     5761 ctttctcctc tcgcccgcca actgcggtgg tgtgcgcgcc aggcgagtcg tgtcggacgc
     5821 ggcggagttc gccctggcgc ggcagctgcg gacgacggaa ggcgctccgc tcggcgacgt
     5881 cttcagtttc gtcagcggcc tgtactttcg aggcaagctg gcgtacgcgc ggcggttcac
     5941 gcggccgcca gacccgaccg accgcgtggt ggcggacgga gtgctggtca tcacgccgaa
     6001 cgccgggctg cgcgctgccg atgtcgccgt gacgcgacag tcgattcggg cgttcgccaa
     6061 ggtcgacatc gacctggaga atccgggtta ccgggggcct ctggagcagg gcgcgcgggt
     6121 actcgcggcc agcgttggac ccgactgcga tgtcgtcctg ctcggtagca tcgcatccgg
     6181 caagtacgtc gaggtcctgc tgccgatctt cggcgagcgc ctcctgtttc cgcccgcctt
     6241 catcgggcgc ggcgacatga gccggggcgg actcatgctg cggtgcgcgg agagcggcct
     6301 cgagctcgac tacgcgccgg tcgccggagc tgtgcggcac gggcagcggc cgccgagatt
     6361 gccgccgctt cggcaacgcg actcggccat tcgcgagaga gtgcgctaga atccggtccg
     6421 aacgagggtt cggcatgtcc gatcggcgca gggaacctga cctcgccccc cctttcgaac
     6481 agctcctgcg ggaactgctg cactgggccc gtgaccaccg tgcattggcg gcccgagtcg
     6541 ggctcgatat agacgactta gtgcaggaca ccgtcgtgcg gcttgctcgc agcggtttcg
     6601 agatacggca tgcgggcgcg gtccgattgt atgctcgggc ttcggtcatg aaccggattc
     6661 gtgacaccgt tcgtcgcgcc gaggagcaac aacggaacgg tgggtacact ccttacgatg
     6721 acgatgatct gtcgttccag agggacctcg agttgttgca gcggctccat cctcgagacc
     6781 ggagggcgat cctcgagcgt cttgaacgac ccttcgacgc tggtgagctg cgcgagctct
     6841 acgatctcgc cggtgagggc gttctgagac aggcgcttcg ctggctgagg caactgttcg
     6901 ggcgcgaccg ggacggggaa ccgccgtccg gacctctgtc acctgcgtag cgactgacgc
     6961 gcggcgggtg tttgaggcta gaatcccccg acccacacgg ccgcgcgcgg cctccgattc
     7021 catctgcgat tcactttcgg tcggcggagt gagtcaactc agtcgaggag cccatgtcta
     7081 cagcgtcaga cgacacaggc ggccgccggc gtgcccctcg ggtcccaggc ccgttcgacg
     7141 ggcggcgaat cggcgagtcg actgttgaca tccgcatcca cggtctcagc gtcgtcgggt
     7201 gcctgattca gtccgctcac gacgt
//
LOCUS       DBNGSH010000170         1848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3069522, whole genome shotgun sequence.
ACCESSION   DBNGSH010000170 DBNGSH010000000
VERSION     DBNGSH010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3069522"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="AC16CY_03850"
     CDS             <1..1071
                     /locus_tag="AC16CY_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3352942.1"
                     /translation="LERAREEERNRAALWPADPIESQSGAIFLAEETQSILGIARRIA
                     PTNVPVLITGETGTGKEILARTIHAYSARAQNTFLPFNCASTPKDMLDSQLFGHRRGA
                     FTGAVEHHQGVVRAAAGGTLFLDEIGDASLEVQPKLLRFLESNEIHPIGETHPTRVDV
                     RVIVATNADLDALVSQGRFREDLFYRLSIVRLHLPPLRERRVEIPTLANHYLQKHAHE
                     CGKGDLRLAEETMEYLVLYKWPGNVRQLANEMRRLAALAEAGAVLMPEHLSPEIAASR
                     RTIPASQRVLEPTEVVVRLDQPMAAAIQHLEQSMMQYALKKCGGRMEQTAAMLGLSRK
                     GLYLKRQRFGIEPPPAAAAATA"
     gene            1093..>1848
                     /locus_tag="AC16CY_03855"
     CDS             1093..>1848
                     /locus_tag="AC16CY_03855"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR3352943.1"
                     /translation="MRIHFVDGTYELFRAHYGAPGAEAPEVGATRGILRSLLALLREP
                     DVTHVACAFDHVIESFRNGLFAGYKTSEGVPADLMAQFPLAERAAAALGLVVWPMVEL
                     EADDALATGAARWAGAPGVEQILICTPDKDMAQSVRGSRIVCFDRMRRRLMDEAGVVA
                     RFGVPPRSIPDWLALVGDSADGYPGVPRWGEKSAAAVLSVYGHLEAIPDRESEWRGVT
                     VRGAASLAASLREHRDEARLYRVLATLREDAPIT"
BASE COUNT          296 a          722 c          554 g          276 t
ORIGIN      
        1 ctcgagcgtg cccgcgaaga ggagcgcaat cgcgccgccc tctggcccgc cgacccgatc
       61 gaaagccaga gcggcgccat cttcctcgcc gaggaaacgc agtccatcct cggtatcgcc
      121 cgtcgcatcg cccccaccaa cgttccggtc ctcatcaccg gcgaaaccgg caccggcaag
      181 gagatcctcg cccgcaccat tcacgcgtac tccgcccggg cacagaacac cttcctcccc
      241 ttcaactgcg cctcgacgcc gaaggacatg ctcgactcgc agctttttgg acatcgtcgc
      301 ggcgccttca ccggagcggt cgagcaccac cagggtgtcg tccgcgccgc cgccggtgga
      361 accttgttcc tcgacgagat cggcgacgcc agcctcgagg tccagccgaa gctgctgcgc
      421 tttctcgagt caaacgaaat tcaccccatc ggcgaaacac accccacgcg cgtcgacgtc
      481 cgcgtgatcg tcgccaccaa cgcggacctg gacgcactgg tctcccaggg ccgcttccga
      541 gaggatctct tctatcgcct cagcatcgtc cgcctccacc tgcccccgct gcgcgagcgc
      601 cgcgtggaga tcccgaccct cgccaaccac taccttcaga agcacgcaca cgaatgcggc
      661 aagggagatc ttcgactggc cgaagaaacg atggagtacc tggtgctgta caagtggcca
      721 ggcaatgtca gacagttggc caacgagatg cgccgcctcg ccgccctggc cgaggccggt
      781 gccgtgctga tgcctgagca cctgtcgccc gagatcgcgg cgtcccgccg cacgatcccc
      841 gcgtcacagc gcgtgctcga accgacggag gtcgtggtgc ggctcgacca gccgatggcc
      901 gcggccatcc aacatctcga acagtcgatg atgcaatacg cgctaaagaa atgtggcgga
      961 cggatggagc agacggccgc gatgctcggc ctgtcgcgaa agggcctgta cttgaagcga
     1021 cagcgcttcg gcatcgagcc cccgcctgcc gcggccgccg ccaccgccta gccccggcat
     1081 cgtcaccgtc ccatgcgcat ccacttcgtg gacggcacct acgagctgtt ccgcgcgcac
     1141 tacggcgctc ccggcgccga ggcgccggag gtcggcgcca cccgcggcat cctccgatcc
     1201 ctcctcgccc tgctccgtga accggacgtc acccacgtcg cctgcgcctt cgaccacgtc
     1261 atcgagtcgt tccgcaacgg cctcttcgcc ggctacaaga ccagcgaggg cgtccccgcc
     1321 gatctgatgg cccagttccc cctcgccgag cgggccgctg ccgccctcgg cctcgtcgtc
     1381 tggcccatgg tcgaactcga ggccgacgac gcgctcgcca cgggcgccgc cagatgggcc
     1441 ggcgcccccg gcgtcgagca gatcctgatc tgcacgccgg acaaggacat ggcccagtcg
     1501 gtccgcggct cccgcatcgt ctgcttcgac cgcatgcgcc gccgcctgat ggacgaagcg
     1561 ggcgtcgtcg ccaggttcgg cgtgccgccc cgatcgatcc ccgactggct cgcgctcgtg
     1621 ggcgattccg ccgacggcta ccccggcgtg ccgcgatggg gcgagaagtc ggccgccgcg
     1681 gtcctctccg tctacggaca tctcgaggcg atccccgatc gcgagtccga gtggcgcggc
     1741 gtcaccgtcc gcggcgccgc cagtcttgct gccagcctgc gcgagcatcg cgacgaggcg
     1801 cgcctctatc gcgtcctcgc cacgctgcgt gaagacgcgc cgatcacc
//
LOCUS       DBNGSH010000171         1771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4088395, whole genome shotgun sequence.
ACCESSION   DBNGSH010000171 DBNGSH010000000
VERSION     DBNGSH010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4088395"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..421
                     /gene="rplP"
                     /locus_tag="AC16CY_03860"
     CDS             2..421
                     /gene="rplP"
                     /locus_tag="AC16CY_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR3352944.1"
                     /translation="MPKKVKYRKQQRGRMTGKAWRGSDVSFGDYGLKAMEPAWITDRQ
                     IEAARVAMTRFIKRGGKIWVRIFPDKPITKKPQETRMGKGKGAPEQWVSVVRPGKILF
                     EMEGVTEAEARQAMSLASAKLPIRTRFAVRFAQEKVS"
     gene            418..606
                     /gene="rpmC"
                     /locus_tag="AC16CY_03865"
     CDS             418..606
                     /gene="rpmC"
                     /locus_tag="AC16CY_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR3352945.1"
                     /translation="MKVAEIREMAVDDLRAKAKELDDELFKLRIQKSMGQLEAPAKVR
                     GIRRERARVKTILREKQG"
     gene            612..866
                     /gene="rpsQ"
                     /locus_tag="AC16CY_03870"
     CDS             612..866
                     /gene="rpsQ"
                     /locus_tag="AC16CY_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR3352946.1"
                     /translation="MGIKAQVTGRVISDKMDKTVVVAVERKIRHDVYGKTQRKTSTFV
                     AHNENNEAKMGDTVAIVESRPLSRRKRWVVTRVVAKALEV"
     gene            872..1240
                     /gene="rplN"
                     /locus_tag="AC16CY_03875"
     CDS             872..1240
                     /gene="rplN"
                     /locus_tag="AC16CY_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR3352947.1"
                     /translation="MIQMRSILDVADNSGARKISVINPIGGSTGRYARLGDIITASVK
                     EATPDGTVKKGQVVKAVIVRTRKEQRRRDGTYIRFDRNAAVLINDQHEPIGTRVFGPV
                     ARELRERRFMKIISLAPEVL"
     gene            1244..1573
                     /gene="rplX"
                     /locus_tag="AC16CY_03880"
     CDS             1244..1573
                     /gene="rplX"
                     /locus_tag="AC16CY_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009753881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3352948.1"
                     /translation="MSRLQSPIRRNDTVMVMTGKDSGKRGRVLKVLPVKNRLIVEGVN
                     LIKRHTRPNPQRNVKGGIVERESALHASNVQLVCPECGKVTRVGHDLRGDRKVRVCRK
                     CEGVVDK"
     gene            1570..>1771
                     /gene="rplE"
                     /locus_tag="AC16CY_03885"
     CDS             1570..>1771
                     /gene="rplE"
                     /locus_tag="AC16CY_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3352949.1"
                     /translation="MSRLKDRYQKDVMPALKKEFGYKNVMAIPKIEKVVINMGLGEAT
                     QNAKLADTGADEIGRITGQKAVV"
BASE COUNT          379 a          516 c          635 g          241 t
ORIGIN      
        1 gatgcccaag aaggtcaagt acaggaagca gcagcgcggc cggatgaccg gcaaggcctg
       61 gcgcggctcc gacgtgtcgt tcggcgacta cggcctgaag gcgatggagc cggcctggat
      121 caccgacagg cagatcgagg cggcacgcgt cgcgatgacg cgcttcatca agcggggcgg
      181 aaagatctgg gtgcggatat tccccgacaa gcccattacg aagaagccgc aggaaacgcg
      241 catgggcaag gggaagggcg cgcccgagca gtgggtgtcg gtcgtcaggc ccggcaagat
      301 cctgttcgag atggagggcg tgactgaagc cgaggcgcgg caggcgatgt cgctggcctc
      361 ggccaagctg cccatcagga cccggttcgc cgtccggttc gcgcaggaga aggtgtcatg
      421 aaggtcgctg aaatccgcga gatggccgtg gacgacctcc gcgccaaggc gaaggagctc
      481 gacgacgagc tgttcaagct gcggattcag aagtcgatgg ggcagctcga ggcgccggcc
      541 aaggtgcgcg gcattcgtcg tgagcgcgcg cgggtgaaga cgatcctgcg cgaaaagcag
      601 ggatgagcag catgggcatc aaagcacagg taaccggcag ggtcatcagc gacaagatgg
      661 acaagaccgt cgtcgtcgcc gtcgagcgca agatccggca cgacgtgtac ggcaagacgc
      721 agcgcaagac gtcgacgttc gtggcccaca acgagaacaa cgaggccaag atgggcgaca
      781 cggtcgccat cgtcgaatcg cgtcctctca gccgccgcaa gaggtgggtg gtgacgcgcg
      841 tggtggcaaa ggcgctcgag gtgtagcagc catgattcag atgcgatcga ttctggacgt
      901 cgcggacaat tccggcgccc ggaagatctc ggtgatcaac ccgatcggcg gatcaaccgg
      961 ccgttacgcg cgcctgggcg acatcatcac cgcgtcggtg aaggaagcca cgccggacgg
     1021 tacggtgaag aagggccagg tcgtcaaggc ggtgatcgtg cggacgcgga aggaacagcg
     1081 ccggagggac gggacctata tccgcttcga ccggaacgcc gccgtgctca tcaacgatca
     1141 gcacgagccg atcggcacgc gcgtgttcgg ccccgtggcg cgcgagctgc gggagcgccg
     1201 gttcatgaag atcatctcgc tggcgccgga ggttctctag gccatgtctc gtctacagtc
     1261 gccgattcgc cgcaacgaca ccgtcatggt catgacgggc aaggacagcg gcaagcgcgg
     1321 ccgggtgctc aaggtgctcc cggtcaagaa ccgcctcatc gtcgaaggcg tgaacctgat
     1381 caagcgccac acgcgcccca acccccagcg gaacgtcaag ggggggatcg tcgagcggga
     1441 gagcgccctg cacgccagca acgtgcagct ggtgtgcccc gagtgcggca aggtcacgcg
     1501 cgtgggacac gacctgcgcg gcgatcgcaa ggtgcgggtc tgccggaagt gcgagggagt
     1561 ggtggacaaa tgagccgcct gaaggatcgg tatcagaagg acgtcatgcc ggccctgaag
     1621 aaggagttcg gctacaagaa cgtgatggcc atccccaaga tcgagaaggt ggtcatcaac
     1681 atgggtcttg gcgaggcgac gcagaacgcc aagctggccg acaccggtgc cgacgagatc
     1741 ggccgcatca ccggccagaa ggcggtcgtg c
//
LOCUS       DBNGSH010000172         7387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1880720, whole genome shotgun sequence.
ACCESSION   DBNGSH010000172 DBNGSH010000000
VERSION     DBNGSH010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1880720"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1063)
                     /locus_tag="AC16CY_03890"
     CDS             complement(248..1063)
                     /locus_tag="AC16CY_03890"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012756140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HIR3352950.1"
                     /translation="MGTTGSPSAVALIPARSGSKRVAGKNVRVLGGHPMLAYTIAAAL
                     ESGVFDSVIVSTDSEAVADIARHYGAEVPFLRPPELAGDTSPDFEWLAYTIFELGSRG
                     RSWDCFSLLRPTSPFRTAGTIRRAWALFTSQDGVDSLRAVEKCAQHPGKMWVVQGQRM
                     FPLLPHGSNGGPLGQPWHSTPYQALPPVYVQNASLEIAWTRVVTEGKSIAGDVLVPFL
                     TEGHEGFDVNDAYDWMLAERLLADGIAGLPAVPQRPYTALTTPNTQLPTPKTG"
     gene            complement(1072..2574)
                     /locus_tag="AC16CY_03895"
     CDS             complement(1072..2574)
                     /locus_tag="AC16CY_03895"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006530471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3352951.1"
                     /translation="MLSTTSAHGRIDLTAVPSVIPNQIGGQNAPAAEGRTFSKIDPAT
                     GREICKVARSNAADIGAAVTRALGAQPAWAATTVVKRGEILRRLAILMQEHRAAIADL
                     VARETGKSVKDARGETDAAIEMGFFVAGEGRRFYGQTTTSAVPNKTALIVRQPLGVAG
                     LIIAANTPIANVAWKAFPALLCGNAAVLKAAEDTPLSAWAFGELARDAGVPDGVYSTV
                     HGLGEEAGAPLVEHPDVAVVSFTGSCEVGRWIAETAGRRLAKTCLELGGKNPLIVCDD
                     AELQNAVTWTLGSAFSNAGQRCAAGSRIIVFDAVYDRFKALLVEAVHRLKVGTADTDD
                     FGPVINEQQMTNMLAAVARAKAAGAAILAGGHRLTGGSYGGGFFVAPTIIEGVRRDDE
                     VSRSELFGPITCLYRVSTFDEAIALANDSPFGLTASIHTQNLNRAMTFLGRIQSGVAV
                     VNGGTYGSEPHLPFGGLRQSGNGWREAGTQALDVYSDLKTIYINHNPDAV"
     gene            complement(2754..3677)
                     /locus_tag="AC16CY_03900"
     CDS             complement(2754..3677)
                     /locus_tag="AC16CY_03900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3352952.1"
                     /translation="MIDCIAGYHLNPWTCGIAKFNAILARHLSVPVVGVNSPDLTNYG
                     RPLLSIKLSEFAADDASALSEWVSARAGQYELFLHAFDGTAIERTIMSAASRVYCGNR
                     ELSHELSPLRPDIVELFCPGTLLNPQRIQKTDLSVFTFGMAHKIRVPLYRRLRDLLEA
                     TGRSYAVYVSTALHENTSFDDSFVVRFEELQTIFNGQVYFMGYLSDTAVFNQLVDCTF
                     LAAFFDKGLRANNTTVNAAMECGCAVITNLDEHSPAGLVHMRNVIDIGRCERLPDASE
                     AERIGRAAREIAHGDYGWDQLVAQLRPVVRR"
     gene            complement(3727..6018)
                     /locus_tag="AC16CY_03905"
     CDS             complement(3727..6018)
                     /locus_tag="AC16CY_03905"
                     /inference="COORDINATES: protein motif:HMM:NF021126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR3352953.1"
                     /translation="MTQGSGARLTRLAPPVAFSVLLLALVVYVFRAIFSGEHSLPGVD
                     AGAHFMWENLTRSALASGLLPHWNPYSLAGTPHMADPQSLVWYPPAMLLRWLPPAAFL
                     SWMAVLHVWLGGVGTLLLGRVVGLSWLAAAAAALCVTLGGTSAARLYNGHLLVINCVA
                     WLPWVLAFSVLSVRRGTLLPHPALVLVMVLQFLAGFPQGSLYIAGAVCVYFLFSIAWP
                     ETPEPGDARWQPLAQLTVLGLLTAGLSSFQLLPSLRLAGETARAAGIPYVDAVAGQWT
                     FASLATAFWPFMGAQSQPPPRDIADSVAYVGWLLTCTVPFAFLDERRRRTAAACTVLV
                     AIAVALALGDTLGLYRLHYALFPGFRLPGRLLFVATVALAILGALGLDQLVATAGRRH
                     WRRLVVPAIVSLAAVSAAAGTALARWDPSGVFPVHGWPWLPVVATAGLGLVVVLGARV
                     GARAALATALVVCGVDVTAFSADGAHPVSIETADAVRRWLGPSSAGRVFSLCPNRIDS
                     ADLASVHRPGLSGRGSVWLSDYVEWLSLTSLADRESDMIGDTPIRRDLLNLAGVSTVV
                     ACRPVAARSLTQVSYAYPVFAYRNDEAWPRAVWTCAVEEVPRSQVAARLRAGRFEDVG
                     HFREPPPDRSGEPGSALETLVGTPSCDAAGRVTVTGQDTLDGRFSADVDAPVAGFVFL
                     SEPFYPERVALVDGRSVDARKANLAFMAVPVPAGRHRLELRYVPSSFNTGLGVTALTM
                     VAWTGASVLTRLRRHHASLVRPY"
     gene            complement(6015..6770)
                     /locus_tag="AC16CY_03910"
     CDS             complement(6015..6770)
                     /locus_tag="AC16CY_03910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3352954.1"
                     /translation="MISIVTPAFNERSTLPALHQRLSESLKHLEWEWVIVDDHSKDDT
                     FAVIERLAAADPRVRGFRLARNSGSHIAITCGLQQSRGNAAALVVSDLQDPPELLVGM
                     VERWSRGAHVVWAVKRQQPGSGPQAWFPAIYYWIMRRLVGLTEMPATGVDFFLIDRVV
                     IDAFLASAGRHVSVFVLIMWLGFRSEFIEYDKQPRAIGQSGWTLARRVKLLVDSVVGL
                     PDFPIWWSRGKPIYAIEAVAESTAAPVPRPLAR"
     gene            complement(6767..>7387)
                     /locus_tag="AC16CY_03915"
     CDS             complement(6767..>7387)
                     /locus_tag="AC16CY_03915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3352955.1"
                     /translation="ACFSFHPRKLLSTGDGGMLTTADAALDRQFRLLRHHGMSVSDTV
                     RHASATVIDEEYPVLGFNYRMTDIQAAIGREQLKRLPDIVARRRELAARYHAGLECCS
                     GVQLTEEPSWARTNWQSYTIRVERGRQRAIMQQLLDEGISTRRGAMNAHAEGAYPPGT
                     WRSAGTLACSEDAQQRGIVLPLFHQLSESNQDRVIDGVRRVCGSRS"
BASE COUNT         1299 a         2502 c         2483 g         1103 t
ORIGIN      
        1 ggccggacca ggcgcccgcg atcatgtaga gcgtgttgat cctcgccagg gccgcgaagg
       61 cagccgcacg ggcgggggcc ggcgcgccgg agtcgagcag gcggcggaac tcgcccaccg
      121 gcacgaactg gagttcgagc gattctccct ggtcggtctg caggtcgccc atcgtaatca
      181 ggcctcaggg cccaggccca tggccccaga cttcaattcc caatccccaa ttcccaaccc
      241 gtgcagctta gcccgtcttg ggagttggga gttgagtgtt gggtgttgtc aatgcagtgt
      301 acggcctctg cggcacggcc ggcaggccgg cgatgccgtc ggcgagcagg cgttcggcca
      361 gcatccagtc gtaggcgtcg ttcacgtcga agccctcgtg cccttcggtc aggaacggca
      421 ccaggacgtc gccggcaatc gacttcccct ccgtgacgac gcgggtccac gcgatttcga
      481 gcgaagcgtt ctggacatac accggcggaa gcgcctggta gggcgtactg tgccacggct
      541 ggccgagagg tccgccgttg ctgccgtgcg gcagcagcgg aaacatccgc tggccctgca
      601 cgacccacat cttcccggga tgctgggcac acttctcgac ggcgcgcagc gaatcgacac
      661 cgtcctgtga ggtgaacagc gcccacgcgc ggcgaatcgt cccggccgtg cgaaacggac
      721 tggtcggcct cagcaggctg aagcagtccc acgaccggcc gcgggagccg agctcgaaga
      781 tggtgtaggc gagccactcg aagtccggcg acgtgtcgcc ggcgagctcg ggtggacgga
      841 gaaacggaac ttcggcgccg tagtggcgcg cgatgtcggc gaccgcctcc gagtcggttg
      901 agacgatgac cgagtcgaac acgccgctct cgagcgcggc tgcgatcgtg taggccagca
      961 tcgggtgccc gccaagcacg cgaacgttct tgccggccac ccgtttcgag ccgcttcgcg
     1021 ccggaatcag cgcgacggca gatggtgaac ctgtcgttcc cactggcgta ttcagacggc
     1081 gtccgggttg tggttgatgt agatcgtctt gagatccgag tagacgtcga gcgcctgtgt
     1141 tcccgcttcg cgccacccgt tgccggactg ccgcagtccg ccaaacggca ggtggggctc
     1201 gctcccgtac gtaccgccgt tgaccacggc cacgcccgac tggatgcgtc cgaggaaggt
     1261 catcgcgcgg ttcaggttct gcgtgtgaat cgacgcggtc aacccgaacg gcgagtcgtt
     1321 cgccagcgcg atcgcctcgt cgaacgtgga cacgcggtag agacacgtga tcggcccgaa
     1381 cagttcgctc cgggacactt cgtcgtcgcg ccgcacgccc tcgatgatcg tcggagccac
     1441 gaagaagccg ccgccgtacg atccccctgt caggcggtgc cccccggcaa ggatggcggc
     1501 gccggccgcc ttcgcccggg ccacggcggc gagcatgttg gtcatctgct gctcgttgat
     1561 gaccgggccg aagtcgtcgg tgtccgccgt gccgaccttc aggcgatgga cggcctcgac
     1621 cagcagtgcc ttgaagcgat cgtagaccgc gtcgaacacg atgatgcggc tgccggccgc
     1681 gcagcgctgc cccgcgttgc tgaacgccga ccccagcgtc cacgtgacgg cgttctgcag
     1741 ctccgcgtcg tcgcacacga tgagcgggtt cttgccgccc agctcgaggc aggtcttggc
     1801 cagcctgcgg ccggcggtct ccgcaatcca tcgccccacc tcgcacgagc ccgtgaagct
     1861 gacgacggcc acgtcggggt gctcgacgag cggcgccccg gcttcctcgc caagtccgtg
     1921 cacggtggag tacacgccgt ccggcacgcc cgcgtcgcgc gccagctcgc cgaaggccca
     1981 cgcggagagc ggcgtgtctt cggctgcttt caacacggcg gcgttgccgc acagcagcgc
     2041 cggaaatgcc ttccaggcga cgttggcgat cggcgtgttg gccgcgatga tgaggccggc
     2101 gacgccgagc ggctgccgca cgatgagcgc ggtcttgttg ggcaccgcgc tcgtggtcgt
     2161 ctggccgtag aaacgcctgc cctcccccgc gacgaagaaa cccatctcga ttgcggcatc
     2221 ggtctcgccg cgcgcgtcct tgacggactt tcccgtctcg cgcgccacga gatcggcgat
     2281 ggccgcgcgg tgctcctgca tcaggatcgc gagacgcctg aggatctcgc cgcgcttgac
     2341 gaccgtcgtc gcggcccagg ccggctgggc cccgagagcg cgggtgacgg cggcgccgat
     2401 gtcggcggca ttcgatctgg cgaccttgca gatctcgcgc ccggtcgccg ggtcgatctt
     2461 cgagaaggtg cgcccctccg cagccggggc attctgcccg ccaatctggt tgggaatgac
     2521 ggagggaacc gccgtcaggt caattcgccc gtgagcagat gtcgtggaga gcacctgaac
     2581 ctatcccggg aacgccttgg acattgaaga tcttcgattg ttgtttgttg aatcgttgtt
     2641 gattggagat tctcgattgg acgattgcac cccaaaccac cgatgaatca tcaatctcga
     2701 atcgcaaatc atcaacgaat caaggatcaa caatcacaaa ccacaaatga actctaccgt
     2761 cgcaccaccg gccgcagctg cgcaaccagc tggtcccatc cgtaatcgcc gtgcgcgatc
     2821 tctcttgccg cgcggccgat gcgctcggcc tcggaggcgt ccggcaggcg ctcgcaccgg
     2881 ccgatgtcga tgacgttgcg catgtggacg agcccggccg gcgagtgctc gtccaggttc
     2941 gtgatgacgg cgcatccgca ctccatcgcg gcgttcaccg tcgtgttgtt cgcccgcagc
     3001 cccttgtcga agaacgcggc gaggaaggtg cagtccacga gctggttgaa gaccgccgtg
     3061 tcggacaggt agcccatgaa gtagacctgc ccgttgaaga tggtctgcag ctcctcgaag
     3121 cgcaccacga aggaatcgtc gaagctcgtg ttctcgtgca gcgccgtgga cacgtagacg
     3181 gcgtagcttc gtcccgtggc ctcgagcaga tcgcgaaggc gcctgtagag cgggacgcgg
     3241 atcttgtgcg ccatcccgaa ggtgaagacc gagaggtcgg tcttctggat gcgctgcggg
     3301 ttcaggagcg tcccgggaca gaacagctcg acgatgtcgg gccgcagggg agagagctcg
     3361 tgcgacagct cgcgattccc gcagtagacg cgcgaagcgg ccgacatgat cgtccgctcg
     3421 atcgccgtgc cgtcgaacgc gtgcagaaac agctcgtact ggccggcgcg cgcactcacc
     3481 cactcgctga gggcgctggc atcgtcggcc gcgaattccg acagtttgat cgagagcagc
     3541 gggcggccgt aattggtcag gtcgggcgag ttgacgccaa ccacggggac gctcaggtga
     3601 cgcgccagga tagcgttgaa cttggcgata ccacacgtcc acgggttgag gtggtatccg
     3661 gcgatgcagt cgatcatctg atctggggtc tcgagtttca gcgcgctcta ttctactagg
     3721 gcgcgtctag tagggcctca ccagtgacgc atggtgccgt cgcaatcggg tcagaacgga
     3781 tgctcccgtc cacgccacca tcgtcagcgc ggtgacgcca aggcccgtgt tgaagctgct
     3841 cggcacgtat cgcagctcga ggcgatgccg ccccgccgga accgggaccg ccatgaacgc
     3901 aaggttcgcc tttcgggcgt ccacggaccg gccatcgacc agcgccactc tctccggata
     3961 aaaaggctcg ctcaggaaca cgaacccggc gacgggtgcg tccacgtccg cgctgaaccg
     4021 gccgtccagc gtatcctgcc ctgtcaccgt gacgcgcccc gccgcgtcgc acgacggggt
     4081 tccgaccagc gtttcgaggg cggagcccgg ctcgcccgat cgatcgggtg gaggctccct
     4141 gaagtgcccg acgtcctcga accgtcccgc gcgaagccgg gcggcaacct gggatcgtgg
     4201 cacctcctcc accgcgcagg tccacacggc cctgggccag gcttcgtcgt tcctgtacgc
     4261 gaagaccggg taggcatatg agacctgcgt cagggatcgc gctgcgacgg gccggcaggc
     4321 gaccaccgta gacacgcccg cgaggttcag cagatcgcgt cgaatcgggg tgtcaccgat
     4381 catgtcggat tcccgatcgg ccagggaagt caggctgagc cactcgacgt agtccgagag
     4441 ccagacgctg cctcgaccgg acaggcccgg acgatggacg gacgcgaggt cggccgagtc
     4501 gatgcgattg gggcagagcg agaagacacg cccggcactc gacgggccca gccagcggcg
     4561 cactgcgtcc gccgtttcga tcgaaacagg gtgtgcaccg tccgccgaaa acgccgtgac
     4621 atcgacgccg cagaccacca gcgctgttgc gagcgccgcg cgggcgccga ctcgggcacc
     4681 gagcacgacg acgaggccca atccggccgt ggccacgact ggcagccacg gccagccgtg
     4741 gaccgggaac acgcccgatg ggtcccaccg ggcgagcgcc gtgcctgcgg ccgcgctcac
     4801 cgccgccagc gagacgatcg ccggaacgac gagccgtctc cagtgtcggc gcccggccgt
     4861 cgcgaccaac tggtcgagcc cgagggcgcc caggatggcg agcgccacgg ttgccacgaa
     4921 cagcagccgc ccgggcaaac gaaacccggg gaacaaggca tagtgcaggc gatacagacc
     4981 cagggtgtcg ccaagagcca aggcaacggc gatcgcgacc agcacggtgc acgccgccgc
     5041 ggtccgccgg cgtcgctcgt cgagaaaggc aaagggaacc gtgcaggtca gcagccaccc
     5101 cacgtacgcg accgaatcgg cgatgtctcg cgggggcggt tgggactggg cgcccatgaa
     5161 cggccagaag gccgtggcga ggctcgcaaa cgtccactgt cccgcgaccg catccacgta
     5221 gggaatgccc gccgcgcggg cggtctcccc cgccagacga agcgatggca gcaactggaa
     5281 cgagctgagt cccgcggtga gcaggccgag cacggtcaac tgcgccagcg gctgccagcg
     5341 cgcgtcgccg ggctccggcg tctccggcca tgcgatggag aacaggaagt acacgcacac
     5401 ggcgccggcg atgtagaggc tcccctgcgg aaagccggcg agaaactgaa gaaccatcac
     5461 gagcaccagc gcggggtgcg ggagcagcgt cccgcgccgc acgctcagca cggagaaggc
     5521 gagcacccag ggcagccacg cgacgcaatt gatgacgaga agatgaccgt tgtagagcct
     5581 ggcagccgac gtcccgccga gcgtgacgca cagcgccgct gcggcggcgg caagccagct
     5641 gagcccgacg accctcccga gcagcagcgt tccgactcct ccgagccaca cgtgcagcac
     5701 ggccatccac gagagaaacg ccgccggagg cagccagcgg agcagcatcg caggcggata
     5761 ccacactaac gactgtggat cggccatgtg gggcgtcccg gcaagggagt acgggttcca
     5821 gtgagggagg agaccggacg cgagtgccga gcgcgtgagg ttctcccaca tgaagtgggc
     5881 ccctgcatca acgcccggca acgagtgctc gcccgagaag attgcccgga agacgtagac
     5941 gactagagcc agcaggagca ccgagaacgc cacgggcggc gccagccgcg tcagccgcgc
     6001 cccggatccc tgcgtcatcg cgccaagggc cggggtacgg gcgccgctgt cgattccgcg
     6061 accgcctcga tcgcatagat cggcttgccg cgcgaccacc agatcgggaa gtcgggcagc
     6121 ccgacgacgg aatcgacgag cagcttcacc ctccgtgcca gcgtccagcc cgactgcccg
     6181 atggcgcgcg gctgcttgtc gtattcgatg aactcgctgc ggaagccgag ccacatgatc
     6241 agcacgaaga cgctgacatg gcggcccgcc gatgccagga acgcgtcgat gacgacgcgg
     6301 tcgatgagga agaaatcgac gccggtggcc ggcatctccg tcaagccgac caaccgccgc
     6361 atgatccagt agtagatcgc ggggaaccag gcctgaggcc ccgatcccgg ctgctggcgc
     6421 ttcacggccc acacgacgtg ggcgcccctg ctccaccgct cgaccatgcc gacgagcagc
     6481 tcgggcggat cctgcagatc ggatacgacc agggcagccg cattcccgcg cgactgctgc
     6541 agcccgcacg tgatcgcgat gtgcgacccc gagttgcgtg cgagacggaa cccgcggacc
     6601 cgcgggtcgg cggcggccag gcgctcgatc acggcgaagg tgtcgtcctt cgagtggtcg
     6661 tccacgatga cccactccca ctcgagatgc ttcagcgact cagagaggcg ctggtgaagg
     6721 gccggcaggg tcgagcgctc gttgaacgcc ggcgtcacga tcgagatcaa gatcggcttc
     6781 cacagactcg ccgtaccccg tcgatcaccc tgtcctgatt cgattccgag agctgatgaa
     6841 acagcgggag cacgattccc ctctgctgtg cgtcttcgct gcaggcgagc gtgccggcgg
     6901 accgccacgt gcctggcgga tatgcgcctt ccgcgtgcgc gttcatcgcg ccgcggcgcg
     6961 tggagattcc ctcgtccagc agctgctgca tgatcgcccg ctggcgtcca cgctccacgc
     7021 ggatggtgta gctctgccag ttggtccggg cccacgacgg ctcctcggtc agctgcacgc
     7081 cggagcagca ctcgagtcca gcgtgatacc gggccgccag ctcgcgacgg cgcgcgacga
     7141 tgtctggcag gcgcttcagc tgctcgcggc caattgccgc ctgaatgtcc gtcatccggt
     7201 agttgaagcc gagcaccggg tactcctcgt cgatcacggt ggccgacgcg tgcctcaccg
     7261 tgtcggacac gctcatgccg tgatgacgca gcagccggaa ctggcggtcg agcgcggcgt
     7321 cggccgtggt cagcatcccg ccgtcgccgg tggacagcag cttgcgggga tgaaacgaga
     7381 agcaggc
//
LOCUS       DBNGSH010000173         3278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4597857, whole genome shotgun sequence.
ACCESSION   DBNGSH010000173 DBNGSH010000000
VERSION     DBNGSH010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4597857"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..446
                     /locus_tag="AC16CY_03920"
     CDS             78..446
                     /locus_tag="AC16CY_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3352956.1"
                     /translation="MSLKIVQHANPPTIALTGRLDTNTAPQLDEVLNQVLSVGGVSRL
                     VFDLSELQYLSSAGIRCFIRARKALEADGGKVAILNPQPAVRKVLDIVKAIPAGGVFR
                     NVAELDAYLDEIQRKVRNPE"
     gene            complement(463..3156)
                     /locus_tag="AC16CY_03925"
     CDS             complement(463..3156)
                     /locus_tag="AC16CY_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3352957.1"
                     /translation="MLIHQLPLDAAFATVRSAPTGLSQTDAAARRLEFGPNRIERLPR
                     TPLTARFLRQFTHLFAALLWVAALLASLADWWMPGQGMLTLAVAIVAVIAVNGAFSFW
                     QEYRAEETMAALQRLLPHEVKALRDGSTVVVPSEDVVPGDVIYLAAGDDVPADCRLVE
                     GFGVRVSNATVTGEARPESRDARPSTEPDVLRSRNVLLAGTSVVSGEARALVFATGMH
                     TVFGGIARLTQTTADAPSPLQKEIATLSRLIGALAVAIGLLVFVIGQFIGLSASISIV
                     FAIGIIVANVPEGLLPTVTLSMAMAARRMARRNTLVRHLPSVETLGCASVICTDKTGT
                     LTENRMKIRSVYADNGYVDPVAAHTAAFAAGHRRFLECAVCCHDLKYAGESGRGRWIG
                     DPMELALAQMATQALGDDAVFDRIDEIPFEPERKRLVTVHRVAGESVLFVKGAPEEIL
                     SRAGRIEVEGRAEGLTAERARAFATAATEMADRGLRVLAFAYRVLPCDYTLADAEQEL
                     VMTALVGFEDPPRPEVSAAVQRCREAGIKVVMVTGDHPHTAMAVAREIGLVQREGAAL
                     LTGDDLGRMSDTQIQLALDAPEIVCARVTADQKLRVVRAFQRKGCIVAVTGDGVNDAP
                     ALRAADIGIAMGISGTDVAREASDVILLDDNFASIVDGVEEGRSVFENIGKFLTYILT
                     SNVPELVPYLAFAFARIPLALTIVQILAVDLGTDMLPALGLGAEPPDSRVMRRPPRSR
                     QDRLLTPGLLVRAYLFLGVLEAAAAMAAFFFVLVASGWQFGQELSTSDVLYREATTAC
                     LTAIVLMQVVNVHLCRSRRTSIVSRPLFGNVLITAGIVAEVAMILVIDYTTPGNSIFG
                     TAPVGYEVWLVVLPLAAAMLILEEIRKASVRSRERAGLVAG"
BASE COUNT          614 a         1114 c         1040 g          510 t
ORIGIN      
        1 ctgccgctac tcacgtctcg acgggtggaa tgtcgtggag atccgccttg gccgcacacc
       61 actgaattga ggtacccatg tcactcaaga tcgtccagca tgcgaacccg ccgaccatcg
      121 cgctgaccgg ccggctcgac acgaacacgg ctccccaact ggacgaggtg ttgaatcagg
      181 tgctgtcagt cggcggtgtt tcgcgcctgg tgttcgacct cagtgagctc caatacctca
      241 gcagcgccgg catccgctgc ttcatccgcg cccgcaaggc gctcgaggcc gatggcggca
      301 aggtggcgat cctgaacccg cagccggccg tgcggaaggt gctagacatc gtgaaggcga
      361 tcccggcggg cggcgtcttc aggaacgtcg cggagctcga cgcctacctc gacgagatcc
      421 agcgcaaggt gcggaatccc gaatgacggg caccactgtt gttcaacccg ccacgagacc
      481 agccctttcc cgcgaacgca cagaggcctt tcgtatctct tcgagtatca gcatggccgc
      541 ggcaaggggc aggacgacga gccagacctc gtacccgaca ggtgccgtgc cgaagatgga
      601 gtttccagga gtcgtgtagt cgatcacgag aatcatcgcg acctccgcga cgatacccgc
      661 ggtgatcagg acgttcccga agaggggccg cgagacgatc gacgtccggc ggctgcggca
      721 gagatgcacg ttcaccacct gcatcagcac gatcgcggtg aggcacgccg tggtcgcttc
      781 gcggtacaga acatcgcttg tcgagagctc ctgaccgaat tgccatcctg acgccacaag
      841 aacgaagaag aaggcggcca tcgccgctgc ggcctcgagc acaccgagaa acagatacgc
      901 ccgcacgagg agccccggcg tcaacagccg atcctgccgg ctccgaggtg gacgccgcat
      961 cactcgcgaa tccgggggtt cggcgccgag tcccagcgcc ggcaacatgt cggtgccgag
     1021 gtccaccgcg aggatctgca cgatggtcag cgccagcggt attcgcgcga acgcaaacgc
     1081 gagatacggg acgagctccg gcacgttgga cgtcaggatg taggtgagaa acttcccgat
     1141 gttctcgaag acactgcggc cttcttcgac cccatcgacg atgctggcaa agttgtcgtc
     1201 gaggagaatc acgtccgagg cttcacgggc cacgtccgtg cccgagatgc ccatcgcgat
     1261 gccgatgtcc gccgcccgca gcgcgggcgc gtcgttcaca ccgtctccag tcacggcgac
     1321 aatacaaccc ttgcgctgga acgccctcac cacgcgcagc ttctgatcgg ccgtgacgcg
     1381 cgcgcacacg atctcgggcg catccagcgc aagctggatc tgtgtatccg acatccggcc
     1441 gagatcgtct cccgtcagca gcgcggcccc ctcgcgctgc acgagcccga tctcgcgcgc
     1501 cacggccatc gcggtgtgcg ggtggtcgcc ggtcaccatg acgaccttga tgcccgcttc
     1561 acggcatcgc tgtaccgcgg ctgacacttc cggtctcggt ggatcctcga atcccacgag
     1621 agccgtcatg accaactcct gctcggcgtc agccagggtg tagtcgcacg gaaggacgcg
     1681 atacgcgaag gcaagcaccc gaagcccccg atcggccatc tcggtcgcgg ccgtggcgaa
     1741 cgccctcgcg cgttcggcgg tcagtccctc ggcacggccc tcgacctcga tccggcccgc
     1801 tcgcgacaga atctcttcgg gggctccctt cacgaacagg acggactcgc cagccacacg
     1861 gtgcaccgtc accaatcgct tgcgctcggg ctcgaagggg atctcgtcaa tgcgatcgaa
     1921 gactgcgtcg tcgccgagcg cctgtgtggc catctgtgcc agggccagct ccatcggatc
     1981 gccgatccac cgtccgcgtc cgctctcgcc ggcgtacttc aggtcatggc agcacacggc
     2041 acactcgagg aaccgacggt gtccggcggc gaacgccgcc gtgtgggcgg caaccggatc
     2101 gacgtacccg ttgtccgcgt acaccgaccg gatcttcatg cggttttcgg tcagcgtgcc
     2161 ggtcttgtcg gtgcaaatca ctgaagcgca gccgagcgtc tcgacgctcg gcaagtgacg
     2221 caccagcgtg tttcgcctgg ccatgcggcg agctgccatg gccatcgaca gcgtcaccgt
     2281 gggcagcagt ccctccggta cgttcgcgac gatgatgccg atggcaaaga cgatgctgat
     2341 cgacgcggaa agaccgatga actggccgat cacgaacacc agcaggccga tagccactgc
     2401 caacgcgccg atcagacggc tcagagtggc gatctccttc tgaagcggcg acggagcatc
     2461 ggccgtagtc tgggtcaggc gcgcgatacc tccgaagacg gtgtgcatcc ctgtcgcgaa
     2521 caccagcgcc cgcgcttcgc cgctgacgac ggacgttccc gccagcagca cgttccggct
     2581 gcgcagtacg tcgggttccg ttgagggtcg agcgtcccgc gattcgggac gagcctcgcc
     2641 agtgacagtc gcgttgctga cacgaacgcc aaagccttcg acaagccggc agtcggcggg
     2701 cacgtcgtcc cctgcggcga gatagatgac atcgccggga acgacgtcct cggaggggac
     2761 cacgacagtc gatccgtccc gtaacgcctt gacctcgtgc ggaagcagac gctgcagcgc
     2821 ggccatcgtt tcttcggcgc ggtactcctg ccagaacgag aaggcgccgt tgacggcgat
     2881 cacggccacg atggcgaccg cgagcgtgag catgccctga cccggcatcc accagtccgc
     2941 cagcgacgcc aggagggccg cgacccacag cagcgcagcg aagaggtgcg tgaactgtcg
     3001 aaggaagcga gcggtgagag gcgttcgagg aagccgctcg atgcggttgg gcccgaactc
     3061 gagccggcgc gccgcagcgt ccgtctgcga caggcccgtc ggagcgctgc gcaccgttgc
     3121 aaacgccgcg tccaggggaa gctggtgaat cagcatcgcg ttcccggagc gtcaacgacg
     3181 cacgcccgat gtcatcacgc agcggcgtgc acgtctctcg aagcgaaggt cggcgcgatc
     3241 tcgaccggcg tcgacacgga gttaccgatg aagcagcc
//
LOCUS       DBNGSH010000174         2462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4597875, whole genome shotgun sequence.
ACCESSION   DBNGSH010000174 DBNGSH010000000
VERSION     DBNGSH010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4597875"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC16CY_03930"
     CDS             <1..246
                     /locus_tag="AC16CY_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352958.1"
                     /translation="GPKDMLKDIEKREGALLEITGLVRKAELSPSGVTLAGGRVRIGG
                     GQPRDPLLSGSSPTGATIDAMLDVEGWNNTAESCPIR"
     gene            complement(285..710)
                     /locus_tag="AC16CY_03935"
     CDS             complement(285..710)
                     /locus_tag="AC16CY_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3352959.1"
                     /translation="MRKTAAVLAVCGFIASAAYVSLGAQAKSASAGVYATEQATKGEA
                     IYKEQCAACHGDNLEGSGPMPPLAGNDFLANWKGKSLGDLFEKVQATMPATAPGTLTA
                     DQTVETLAFMLKAGKYPAGTAPLEGKMEALLPIAIDAPK"
     gene            complement(771..1418)
                     /locus_tag="AC16CY_03940"
     CDS             complement(771..1418)
                     /locus_tag="AC16CY_03940"
                     /inference="COORDINATES: protein motif:HMM:NF025255.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM7S3/TM198-like domain-containing protein"
                     /protein_id="HIR3352960.1"
                     /translation="MADSIMFPAIYQLPIGIILLLSGIVACFFGYRMFRLVLAIFGFI
                     FGALAASSLFGPSDTAWMIGAGVAGGLVGAGLFSAAYFVGVALVGAGLGTLLAHLAFS
                     ASDREPHVLIVMFCAIAGAVASTYLQRYVVILVTAFGGALTLVHGALALVGSRVTLPG
                     LGGDGVWLPYPFDPMPGQRWVPIAWAVVGLIGAGVQLGWTGGEKGRIGRAKKKTG"
     gene            complement(1419..2015)
                     /gene="recR"
                     /locus_tag="AC16CY_03945"
     CDS             complement(1419..2015)
                     /gene="recR"
                     /locus_tag="AC16CY_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3352961.1"
                     /translation="MSLPEPLARLVEQLQKLPGIGAKGAQRLAFHILRHPREDADELC
                     AAIRDVKERVTYCSTCNNITDADPCMFCTSATRDHRLVCVVEEPQNVTVVEKTREFKG
                     VYHVLMGALSPLQGVGPDDLKIAGLLARVGGGGIEEVILATNPTVEGEATALYLARLL
                     KPLGVRVTRIAMGIPVGSDLEYADEVTMTRAMENRRDL"
     gene            complement(2029..2328)
                     /locus_tag="AC16CY_03950"
     CDS             complement(2029..2328)
                     /locus_tag="AC16CY_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR3352962.1"
                     /translation="MDIQSVMTQARQMQERLRKQMDEMRAEATAGGGMVTVVVNGAKQ
                     LQSIRLDPAVVSRDDVEMLQDLILAAVNDAQRKIDEQMERSMSGLMGGLKLPGLF"
BASE COUNT          420 a          887 c          755 g          400 t
ORIGIN      
        1 ggcccgaagg acatgctcaa ggacatcgag aaacgcgaag gcgcgctgct cgaaatcacg
       61 gggctggtac ggaaggccga gctgtcaccg agcggcgtca cgctcgcggg cggccgcgtc
      121 cgcatcgggg gcggtcagcc cagggatccg ctcctctcag ggtcgagccc cacgggcgcg
      181 accatcgacg ccatgctcga tgtcgagggg tggaacaaca ccgccgagtc ctgcccgata
      241 cggtgacgca acggtccggc tgaagccgga cactacttcg gccactactt cggcgcgtcg
      301 atcgcgatcg gcagcagcgc ttccatcttg ccttcgagcg gcgccgtgcc tgcgggatac
      361 ttgcccgcct tgagcatgaa ggcgagcgtc tcgaccgtct gatccgccgt caacgttccc
      421 ggcgccgtcg ccggcatcgt cgcctgcacc ttctcgaaca ggtcgccgag gctcttgccc
      481 ttccagttag ccagaaagtc gttcccggca agcggcggca tcggaccgga gccctccagg
      541 ttgtcgccgt ggcacgcggc gcactgctcc ttgtagatcg cttcgccctt ggtcgcctgc
      601 tcggtcgcgt acacgcccgc gctcgccgac ttcgcctgcg cgccgagcga cacgtacgcc
      661 gcagacgcta tgaacccgca caccgccaga accgctgccg tctttctcat gaaacctcgt
      721 cgctcgtcca ctatcccctg cccctgaccc ctgacccctg ccccctgatt ttatccggtt
      781 ttcttcttcg cccgcccgat ccgccccttt tcgccgccgg tccaccccaa ctgcacgccg
      841 gcgccaatca accccacgac ggcccacgca atgggcaccc accgctgacc cggcatcgga
      901 tcgaacgggt acggcagcca cacgccgtcg ccgccaaggc cgggcagcgt cacgcggctt
      961 ccaaccagcg cgagcgcgcc gtggacgagc gtcagcgctc cgccaaaggc cgtgaccagg
     1021 atcacgacgt accgctgcag ataggtggac gccacggcgc ccgcgatggc gcagaacatg
     1081 acgatgagca cgtgcggctc gcggtcgctc gccgaaaatg ccaggtgcgc cagcagggtt
     1141 ccgagaccgg cgccgaccag cgcgacgccg acgaagtacg ccgcgctgaa caggcccgct
     1201 cccacgagcc cgcccgccac gccggcgccg atcatccacg ccgtgtcgct cgggccgaac
     1261 agcgagctgg ccgccagcgc gccgaagatg aagccgaaga tcgcgagcac gaggcggaac
     1321 atccggtagc cgaagaaaca ggcgacgatg ccactgagca gcaggatgat gccgatgggc
     1381 agttggtaga tggcgggaaa cattatcgaa tcagccaatc acaagtctct cctgttttcc
     1441 atcgccctcg tcatcgtcac ttcgtcggcg tattccagat cggagccgac cgggatcccc
     1501 atggcaatgc gcgtcacgcg cacgccgagc ggcttgagga gccgcgccag gtagagcgcc
     1561 gtcgcttccc cttcgaccgt cgggttcgtc gccaggatca cttcttcaat cccgccgccg
     1621 cccacgcgcg cgagcagtcc cgcgatcttc aggtcgtcgg gcccgacgcc ctgcagcggc
     1681 gacagcgcgc ccatcagcac gtgatagacg cccttgaact cgcgcgtctt ctcgaccacc
     1741 gtcacgttct gtggctcctc gacgacgcag acgaggcgat gatccctggt cgcgctcgtg
     1801 cagaacatgc acggatcggc gtcggtgatg ttattgcagg tcgaacagta ggtcacgcgc
     1861 tccttgacgt cgcggatggc ggcgcacaac tcgtcggcgt cctcgcgcgg atgccgcagg
     1921 atgtggaagg cgaggcgctg cgcgcccttg gcgccgatgc cggggagttt ctggagctgt
     1981 tcgaccagcc tggcgagagg ttccggtaac gacaagacta atggaccact agaagagccc
     2041 cggcagcttc agaccgccca tgaggcccga catcgaccgc tccatctgct cgtcgatctt
     2101 gcgttgcgca tcgttgaccg ccgcgaggat caggtcctgc agcatctcca catcgtccct
     2161 ggagacgacg gcgggatcga gacggatcga ctggagctgc ttggcgccgt tgacgacaac
     2221 cgtgaccatg cccccgccgg ccgtggcttc cgcgcgcatc tcgtccatct gtttccgcag
     2281 gcgctcctgc atctgcctgg cctgcgtcat gaccgactga atgtccattg actagagctc
     2341 ctcgacgtcc ctgatctcgg ctgggaagac gtcgagcaat gcctgcacgc ctgacgaaga
     2401 gagcgcctcg gccttgaggt cccgcctggc cggccctgcc gccggttgtg acgccggcag
     2461 tg
//
LOCUS       DBNGSH010000175         4250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_13185, whole genome shotgun sequence.
ACCESSION   DBNGSH010000175 DBNGSH010000000
VERSION     DBNGSH010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_13185"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC16CY_03955"
     CDS             <1..294
                     /locus_tag="AC16CY_03955"
                     /inference="COORDINATES: protein motif:HMM:NF036772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HIR3352963.1"
                     /translation="ERPYRIDRLLTQLLLSGVLARARALVFGEMRGCEEPGGAASVTE
                     VISRITNACDGPVILGFPSGHTTGPTWTLPLGVAVRVSTRPRPAIVVEDAPVE"
     gene            287..1696
                     /gene="mpl"
                     /locus_tag="AC16CY_03960"
     CDS             287..1696
                     /gene="mpl"
                     /locus_tag="AC16CY_03960"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR3352964.1"
                     /translation="MSDRKRIYLIGICGTAMATLAALLKSRGHDVRGSDQNVYPPMSD
                     FLIGQDIHALSGYAADHITGDIDLVVVGNAISRGNPELEEVLDRKIRYCSLPEAIREF
                     FLWGSRSIVLSGTHGKTTTTALTSWLLTSGGRDPSMLVGGIALNFGNEGSSYRVGAGR
                     DFVLEGDEYDSAFFDKTAKFLKYLPDVAVIGNVEFDHADIYADLDEVLLAFRRLVTLV
                     PRSGLLVLGADSPHAVALAKAAVSPVETFGLAGEATWQADAIEHRDGRTHFVVRRKGE
                     PYGRFESPLLGVHNVRNALAAMAVGSYTGLGPSELAEGLRTFKGIKRRLEVVGVADGV
                     TVLDDFAHHPTAVHETLSALRAGYPDRRVWAVFEPRSASSCRRVFQDDFARAFGAADE
                     VILAPVFRSTLPEAERLDTGQLVEQLRARGRHARGAGDNDDIVRAIVDEHRSGDVVVL
                     MSNGGFGGIHRKLLRALGA"
     gene            1693..3324
                     /gene="pxpB"
                     /locus_tag="AC16CY_03965"
     CDS             1693..3324
                     /gene="pxpB"
                     /locus_tag="AC16CY_03965"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HIR3352965.1"
                     /translation="MTGARIREAGDSALLVELDAVIDPEINARVIAIAAALQSERLAG
                     VRDVVPTYRSVALHFDPLVIGVDAVRAAVERALDARPRRAQTRSIEVPVVYGGDAGPD
                     LGEVAGWAGLAPDVVAQLHASATYRVFMLGFLPGFPYLASVDERIAAPRRAVPRVRVP
                     AGSIGIAGRQTGIYPMESPGGWNIIGRTGVRLFDPSRVPPALFQPGDAVRFLRSSTTE
                     VPIDDVVPAATSATVNAASRSVTVLRPGLFTTVQDGGRWGHQAYGVPVSGALDYVSHR
                     VANAVLGNAPEAATIEVTLSGPELRFEHDAKVAVAGADLQAAVDGARLPLGAAAECRA
                     GGVLRFGERRSGTRAYVAFDGGVDVTPVLGSRATHVRAGLGGYRGRALAAGDRLTLGP
                     PGGLQARAVRSPGISITGGAKLRVLPGPQQEFFPESALALLEESRFVVTPNSDRMGYR
                     LSGARLPRIENMEMISDAAFAGGIQVPASGEPILLMADRQTTGGYPQIAVVVTADLAK
                     AGQLAPGDWVEFVRCSRAEAIDALAAQEAMFGASR"
     gene            3311..>4250
                     /locus_tag="AC16CY_03970"
     CDS             3311..>4250
                     /locus_tag="AC16CY_03970"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3352966.1"
                     /translation="MPPGRTALSPREGVRLHAFSTMGVGGPARWFYTAERPAEIRSAH
                     QWCAEQNVPMFVLGGGSNIVVADEGFAGLVLHVGVMGVSMAADSGDTIVTAGAGEPWD
                     PLVRILVEQGLGGVECLSGIPGTVGGTPIQNVGAYGQEVASCIDRVVVFDRVRGEFGE
                     IATADCAFSYRMSRFKGTDAARFVVCQVVFRVRPGRPTVAYPDLVTELENRRIDTPGL
                     PDVREAILAVRRRKGMVLDAHDPDTRSVGSFFMNPIVAAADRERIASVSGERVPGFAA
                     GGDRVKIPAAWLIERSGFVKGHADGSVGLSTKHPLAI"
BASE COUNT          623 a         1355 c         1527 g          745 t
ORIGIN      
        1 gagcggccgt accgcatcga tcgcctgctg acgcagcttc tcctgagcgg cgtgctggcg
       61 cgcgcacgcg cgctggtgtt cggcgagatg cgcggatgcg aagaacccgg cggcgccgcc
      121 tccgtcacgg aagtcatttc gaggatcaca aacgcgtgcg acggtccggt gattctcggt
      181 ttcccctccg gccacacgac gggccccacc tggacgctgc cgctcggcgt ggccgtgcgc
      241 gtctccacca ggccacggcc ggccatcgtt gtcgaggacg cacccgttga gtgaccgcaa
      301 acgcatctac ctgatcggca tctgtggcac ggccatggcc acccttgcgg ccttgctcaa
      361 gagccggggg cacgacgtac ggggatcgga tcaaaacgtc taccctccga tgagcgactt
      421 cctgatcggc caggatattc atgcactctc gggatacgcg gcggaccata tcacggggga
      481 catcgatctg gtcgtcgtgg gcaatgccat ctccaggggc aacccggagc tggaggaggt
      541 gctcgatcgg aagatccggt actgctccct gcccgaggcc atccgcgagt tcttcctgtg
      601 gggatcgcgc tcgatcgtgc tgagcggcac gcacggaaag acgacgacca cggcgttgac
      661 gagttggctg ctgacgagcg gcggccgcga cccgtcgatg ctcgtgggcg ggatcgcgct
      721 gaacttcggg aacgagggct cgagctatcg cgtgggggcg gggcgcgatt tcgtcctcga
      781 gggcgacgag tacgacagcg cgttcttcga caagacggcg aagttcctca agtacctgcc
      841 cgatgtcgcc gtcatcggca acgtcgagtt cgaccatgcg gacatctacg ccgacctcga
      901 cgaggtgctc ctggcgttcc gccgtctcgt gacgctcgtc ccccgtagcg gcctgctcgt
      961 cctcggggcc gacagtccac atgctgtcgc gcttgcaaag gccgcggtga gcccggtcga
     1021 gaccttcggg ctcgccggcg aggccacgtg gcaggccgac gcgatcgaac atcgcgatgg
     1081 aaggacgcac ttcgtggtgc gacgaaaagg ggagccctac ggccgcttcg agtcgccgct
     1141 cctcggcgtg cacaacgtgc ggaacgcgct ggccgccatg gccgtggggt cctacacggg
     1201 acttggaccc tcggagctgg ccgagggcct gcggacgttc aagggaatca agcgcaggct
     1261 cgaggtcgtc ggagtggcgg acggtgtgac cgtcctggat gatttcgcgc atcacccgac
     1321 cgcggtgcac gagaccttgt cggcgctgcg cgccgggtat ccggatcgcc gggtgtgggc
     1381 cgtgttcgaa ccgcgatcgg cctcgtcgtg ccggcgcgtg tttcaggacg acttcgcgcg
     1441 tgccttcggc gcggcagacg aggtgatcct cgcgccggtg tttcgatcga ccctgccgga
     1501 ggccgagcgg ctcgacaccg ggcaactggt ggagcagttg cgcgcccgcg gacggcacgc
     1561 ccgcggcgcc ggtgacaacg atgacatcgt ccgtgcgatt gtcgacgaac accggtccgg
     1621 cgacgtcgtc gtgctgatgt ccaatggcgg cttcgggggg atccaccgca agctgctccg
     1681 ggcgctcggc gcgtgaccgg ggcgcggatc cgcgaggcag gcgattcggc cttgctcgtc
     1741 gaactggatg cggtcatcga ccccgagatc aacgcgcgcg tgattgccat cgctgcggcc
     1801 ctccaaagtg agcggctggc cggcgtgcgc gacgtcgtgc cgacgtatcg ctccgtggcg
     1861 ctccacttcg atccgctggt gatcggcgtc gacgcggtgc gggccgcagt cgagcgcgct
     1921 ctcgatgccc ggccacggcg ggcgcagacg cgctcgatcg aggtgcccgt cgtctacggg
     1981 ggcgatgccg ggccggatct gggtgaggtc gccggctggg cagggctcgc accggacgtg
     2041 gtcgcgcagc ttcacgcgag cgcgacctac cgagtgttca tgctcggatt ccttcccgga
     2101 ttcccgtatc tggcctcggt tgacgagcgc atcgccgcac ctcgccgtgc cgtgccgcgt
     2161 gtccgcgtac cggcgggttc gattggaatc gccggtcggc agaccggcat ctacccgatg
     2221 gagtcgcccg gcggctggaa catcatcgga cgcaccggtg tgcgcctgtt cgatccctcc
     2281 agggttccgc ccgcgctctt ccagccgggc gatgcagtac gcttcctccg gtcctcgacg
     2341 actgaggtcc cgatcgacga cgtggttccg gccgcgacat ccgccaccgt gaatgccgcg
     2401 tcccggtcag tgacagttct gcggccaggt ctcttcacga ctgtccagga cggaggtcga
     2461 tggggacacc aggcgtatgg tgttcctgtg agcggcgcgc tcgactacgt atcgcatcgc
     2521 gttgcaaacg ccgttctcgg aaacgcaccc gaagccgcga caatcgaggt gacgctgtca
     2581 ggtcccgagc tgcgattcga gcacgacgcg aaggtcgcgg tggccggcgc cgatctgcag
     2641 gcggccgtcg acggggctcg attgccattg ggcgccgcgg cagagtgccg tgccggcggg
     2701 gtgcttcgat ttggtgaacg ccgttccggg acgagggcct atgtagcctt cgacggcggc
     2761 gtcgatgtga ctccagtgct cgggagccgt gccacgcacg tgcgcgcggg cctgggcggt
     2821 taccgcgggc gagcgctcgc ggccggcgat cgactcacgc ttggtccccc gggcgggctt
     2881 caggcccggg cggttcggtc gcctggtatc tcgatcaccg gcggtgcgaa gctccgagtc
     2941 ctgcctgggc cccagcagga gttcttcccc gagtctgcgc tggctctgct ggaagaatct
     3001 cgcttcgtcg tcacaccgaa ctcggatcgc atggggtacc gtttgtcggg tgccaggctg
     3061 ccgaggatcg aaaacatgga gatgatctcg gatgcggcat ttgccggcgg catccaggtg
     3121 cccgcctcgg gcgagccgat cctgctgatg gccgatcgac agaccacggg cgggtatccg
     3181 cagatcgcgg tcgtcgtgac cgcggacctg gcaaaggcag ggcagctggc tccgggagac
     3241 tgggtcgagt tcgtgcgctg ttcacgagca gaggcgatcg acgcgctggc ggctcaggag
     3301 gccatgttcg gtgcctcccg gtaggaccgc gctgagcccg cgcgagggcg tgcggcttca
     3361 tgccttcagc accatgggcg tcggcggacc cgctcgctgg ttctacacgg ccgaacgacc
     3421 ggccgagatc cggtcggcgc atcagtggtg cgccgagcag aacgtcccga tgttcgtgct
     3481 cggcggaggc agcaacatag tggtcgccga cgaggggttc gccgggctcg tgttgcacgt
     3541 gggagtcatg ggagtgtcga tggcggccga ctccggcgac acgatcgtga ccgccggggc
     3601 cggtgagccg tgggaccccc tggtcaggat cctggtcgag cagggcctgg gcggcgtgga
     3661 atgtctttcg gggataccgg ggacggtcgg aggcacgccg atacagaatg tcggcgcata
     3721 cgggcaggag gttgcgagct gtatcgatcg cgtcgtcgtg ttcgaccgcg tcaggggcga
     3781 gttcggcgag attgcgacgg cggactgcgc tttttcgtat cgcatgagcc gcttcaaggg
     3841 gaccgacgcc gctcgcttcg tcgtctgtca ggtggtgttt cgcgtgcggc ccggaaggcc
     3901 gacggtggcc tatccggacc tggtaaccga attggagaac cggcggatcg acacacctgg
     3961 cttgccggac gttcgcgagg cgattctggc ggtacggcgt cgaaagggta tggtgctcga
     4021 cgcgcacgac ccggacacgc gcagcgtggg atcgttcttc atgaacccga tcgtggcggc
     4081 cgccgatcgc gagcggatcg cgtccgtgtc tggcgagcgc gttccgggct ttgctgccgg
     4141 aggggatcgc gtgaagatac cggctgcctg gctcatcgag cgatcgggtt tcgtcaaggg
     4201 tcacgctgac ggttcggttg gtctctcgac gaagcatccg ctcgcgatcg
//
LOCUS       DBNGSH010000176         5402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4088913, whole genome shotgun sequence.
ACCESSION   DBNGSH010000176 DBNGSH010000000
VERSION     DBNGSH010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4088913"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="AC16CY_03975"
     CDS             complement(<1..889)
                     /locus_tag="AC16CY_03975"
                     /inference="COORDINATES: protein motif:HMM:NF021653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HIR3352967.1"
                     /translation="MSKEMIISSSAHETRVAILEDDQVAEIFIERERSRGVVGNVYKG
                     RVSKVLPGMQSAFVDLGLERDGFLYVSDVIAAFEELDRFETEEEEVSAQPNGNGGSGS
                     GSRRDRPSSHGGRRDRDREKGPEPKIEELLKEGQEIIVQVAKEPLGTKGARLTSHATM
                     PGRFLVFMPTVDHVGVSRKIESREERSRLRGIVREFREQTGFTGGLIIRTAAAGRPKE
                     DIVSDLSYFHMVWTEIRQKAESSRAPAVVYQEQSLVAKLLRDLLTEDFTAIRIDNEPE
                     YRRICELVERIMPALAPKVR"
     gene            complement(1026..2108)
                     /gene="rodA"
                     /locus_tag="AC16CY_03980"
     CDS             complement(1026..2108)
                     /gene="rodA"
                     /locus_tag="AC16CY_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR3352968.1"
                     /translation="MFERRLFYHVDWAMLAAILALCLIGVVQIYSATGGSSLYITQLY
                     AVALGVLALLVCLTIDYRTLADKSHFIYLGTVALLVFVLFFGAVRGGSRRWIDFGSLN
                     LQPSEFAKVAVALVLAKFFGDARRPAVTTGDLIIGGALTAVPFLAIYQQPDLGTAATL
                     LPVLFIMAFVAGMPMRIFGILAIIAVLAAPLAWVFALKDYQKERVSTFLDPEADARGA
                     GYQQLQARITTGSGGPWGKGFQKGTQGQLRFLPVAHNDFIFSVLAEEHGFAGVVVALG
                     LYLFVIHRSIGTAWLAKDRLGAYLVLGVLASFTFQVLYNIAMSAGLLPVKGLTLPLMS
                     YGGSSMIATLAGFGLVLNVRMRRFTN"
     gene            complement(2119..4056)
                     /gene="mrdA"
                     /locus_tag="AC16CY_03985"
     CDS             complement(2119..4056)
                     /gene="mrdA"
                     /locus_tag="AC16CY_03985"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR3352969.1"
                     /translation="MQLTQGENRRRIGERLVVLHVGVIVAFAALGVCFWVLQIVQNDK
                     YEEMAENNHQRTLALRAPRGLLYDRDGTLLVENRPSFTISIERELSQDLERTGRMLAE
                     VAGLDVKQVLQTIERHLSEPSYRPILVVEDATLQQVAAVSARRLDFELPDVMVEQVPT
                     RKYPADALAAHLFGYVSQANESQIGDGIASGAIVGQQGIERVYNKLLMGQDGAKRVVV
                     NSVGREMDTLDEIKPVEGRRVQLTIDYDLQKAAEDGFRHAGFNGAALIMDPRNGEVLT
                     YVSLPSYDPNDFAAGVNRTTWAALITDKLRPLTNRVIQSRYSPGSTFKIVVATAALEE
                     GVVDADHRVTCNGGATFFGRYYRCHLKGGHGSVDMRHAIEKSCNVYFYTLGNMLGVDR
                     IYKWAEKLGLAGRTGIDLPNESDSIVPNTEWKLKRTGERWYPGETISVSIGQGQVTVT
                     PASLAVMIATVANGGTRVTPHVIKAVDEGKGWTAVPAPAVADTVAFKPATLSALRDGL
                     WMVVNASGTGGRARIPGRDVAGKTGTAQIISNEGRARAKGTDRDLRDHGWFVFFAPRD
                     NPQIAGVIFGEHNLHGYLGAPIAKHVIETFFAKKEGRPLPTLTDRPAPAPPDEDPDSP
                     PALPPVIDPDNQPATTVARSP"
     gene            complement(4069..4575)
                     /gene="mreD"
                     /locus_tag="AC16CY_03990"
     CDS             complement(4069..4575)
                     /gene="mreD"
                     /locus_tag="AC16CY_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR3352970.1"
                     /translation="MKAAVVLAAVALALALQTTLARLVVGGTTTIDLVLVAVVSVALV
                     MGPVGGMLAGSVAGLIQDALSSGVLGIGGLSKAIVGFVAGAIGQQFILTSAVPRFVMF
                     VGATAGHAALFMGAYVALGLRMFPSPWKAVASQAIGNAVVGVVSFAIVEAIPGILDRR
                     RAGRRSRR"
     gene            complement(4572..>5402)
                     /gene="mreC"
                     /locus_tag="AC16CY_03995"
     CDS             complement(4572..>5402)
                     /gene="mreC"
                     /locus_tag="AC16CY_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3352971.1"
                     /translation="MAVLDIRQRTGWLFLAVIVGHILLISAQVNTNRGVPVLEEATVG
                     VFAEVQRAASGLIGAVRSGWTDYVALRGVSDENARLREEIASLKISLQQQRAQAEQSA
                     TLQALLDLRSTTKLSTTAAAVIAGGASPEFRTITIDKGTGDGMQPDMAVLSPAGVVGR
                     VITPGVRASKVQLLIDRNAAVGALVERSRAQGVVTGTGEDRLKLNHLAGAADIVVGDR
                     VVSSGIEGIYPKGFVIGQIESIERQAGEFTNVVVRPAVEFSSLEAILVVLTPPAVEAP
                     "
BASE COUNT          940 a         1844 c         1727 g          891 t
ORIGIN      
        1 gcctcacctt cggcgccagc gccggcatga tgcgctccac cagctcgcag atgcgacggt
       61 actccggttc gttgtcgatg cggatcgccg tgaagtcttc ggtcagcagg tcgcgcagca
      121 gcttggcgac caggctctgc tcctggtaga cgaccgccgg cgcacgcgat gattccgcct
      181 tctggcgaat ctccgtccac accatatgga agtagctcag gtcggagacg atgtcttcct
      241 tggggcggcc ggcagcggcc gttcggatga tcagcccgcc ggtgaacccg gtctgctcgc
      301 ggaactcgcg aacgatgccg cgcaggcgcg aacgctcttc acgcgactcg atctttcgcg
      361 acacgccgac gtggtcaacc gtcggcatga acaccaggaa gcggccgggc atcgtggcgt
      421 gagaggtgag ccgtgcaccc ttcgtcccga gcggctcctt cgccacctgg acgatgatct
      481 cctggccttc tttgaggagc tcctcgatct tgggttcggg ccccttctcg cgatcgcggt
      541 cgcgtcgtcc gccatgactc gacgggcgat cgcggcgcga tccggagccg gatccgccgt
      601 tgccgttcgg ttgcgctgac acctcttcct cttcggtctc gaagcgatcg agctcctcga
      661 aggcggcgat cacgtcgctt acgtagagga agccgtcacg ttcgagcccg agatcgacga
      721 aggccgactg catacccggc agcaccttcg agacccggcc cttgtaaacg ttgccaacca
      781 ctccgcgcga tcgctcgcgc tcgatgaaga tttcggcgac ctggtcgtcc tcgagtattg
      841 cgacccgtgt ttcgtgggcg ctcgaggaga tgatcatctc cttgctcatg cccgtcactc
      901 tccgcgggct gcatgcgtgg acggattccc gccgcgcgtg cctcgaagca cgtcgtcagg
      961 agagcggcca gccacatgat catgagtgca gccctgtaag gctctgggct gtgcccagag
     1021 ccgatctagt tcgtaaatcg acgcatccgt acgttgagta cgaggccgaa ccccgccagc
     1081 gtggcaatca tggaggagcc gccgtagctc atgagcggaa gcgtcagtcc cttgaccggc
     1141 aagaggccgg ccgacatcgc aatgttgtac agcacctgga acgtgaagct ggccagcact
     1201 cccagcacca ggtacgcgcc gagacggtcc ttcgcgagcc aggccgtccc gatcgatcgg
     1261 tgaatcacga acagatacaa ccccagcgcc acgacgacgc cggcaaaccc gtgctcctcg
     1321 gccagaaccg agaagatgaa gtcattatgt gccactggaa ggaaacgcag ttggccctgc
     1381 gttcccttct gaaatccctt tccccacggc ccgcccgagc cggtcgtgat gcgggcctga
     1441 agctgttggt acccggcgcc ccgggcgtcc gcctcagggt ccaggaaggt cgacacgcgc
     1501 tctttctggt agtccttgag cgcgaatacc cacgccagag gcgccgccag cacggcgatg
     1561 atggccagga tgccgaagat gcgcatcggc atgccggcca cgaacgccat gatgaacagc
     1621 accgggagca gcgtcgccgc cgtgccgagg tcgggctgct ggtagatcgc cagaaacggc
     1681 accgccgtga gtgcgccgcc gatgatcaga tcgcccgtcg tcacggcagg ccgccgggcg
     1741 tcgccgaaga acttcgcgag caccagcgcg acggcgactt tcgcgaactc cgagggttgc
     1801 aggttgaggg aaccaaaatc gatccagcgc cgcgacccgc cgcgcacggc gccaaagaac
     1861 agcacgaaca cgagaagcgc aacggttccg aggtagatga agtgcgactt gtcggcgagc
     1921 gttcggtagt cgatggtcag gcacacgagc agcgcgagca caccgagcgc gacggcgtac
     1981 agctgtgtga tgtagaggct ggagccgccg gttgcgctgt agatctgcac gacgccgatc
     2041 aggcagagcg cgaggatggc cgccagcatg gcccagtcca cgtgatagaa gagacggcgc
     2101 tcgaacattg gctacaggct acgggctacg ggctacggtc gtggccggct gattgtcggg
     2161 gtcgatcacc ggcggcaggg ccggcggtga atcgggatcc tcgtcaggcg gcgcgggcgc
     2221 ggggcgatcc gtcaacgtcg ggagcggtcg tccttccttc ttggcgaaga acgtctcgat
     2281 gacgtgcttc gcgatcggcg cgcccaggta accgtgcagg ttgtgctcgc cgaaaatcac
     2341 gcccgcgatc tgcgggttgt cacgcggcgc gaagaagacg aaccacccgt gatcgcgcag
     2401 atcccggtcg gtgcccttgg cccgggcacg cccctcgttg gagatgatct gggcggtgcc
     2461 ggtctttccc gcgacgtcgc ggccggggat gcgcgcccgg ccgccggtgc cggaggcgtt
     2521 gacgaccatc cagagcccgt cccgaagcgc ggaaagagtc gcgggcttga acgccacggt
     2581 gtcggccacg gcaggcgcgg gcaccgccgt ccaccccttg ccttcgtcaa cggctttgat
     2641 cacgtgcggc gtcacgcgcg tgccgccgtt ggccaccgtc gcaatcatga ctgcgaggga
     2701 agcaggcgtc acggtgacct ggccctgccc gatcgaaacc gagatcgtct cgcccgggta
     2761 ccaccgctcg cccgtccgct tcagcttcca ctcggtattc ggcacgatgc tgtccgactc
     2821 gttcggcagg tcgatgccgg tccggccggc caggccgagc ttctccgccc acttgtagat
     2881 cctgtccacg ccgagcatgt tgccgagcgt atagaagtac acgttgcacg acttctcgat
     2941 ggcgtggcgc atgtcgacgg agccgtgtcc gcccttgagg tggcaccggt aatagcggcc
     3001 gaagaacgtc gcgccgccgt tgcaggtgac acgatggtcc gcatcgacga cgccttcctc
     3061 gagcgccgcc gtcgccacga cgatcttgaa cgtggagccg ggcgaatagc ggctctgaat
     3121 cacgcggttc gtcagcggcc gcagcttgtc ggtaatgagc gccgcccatg tggtgcggtt
     3181 gactcccgcg gcgaagtcgt tgggatcgta cgatggaagg ctcacgtagg tcaggacttc
     3241 gccatttcgg ggatccatga tcagcgccgc accgttgaac cccgcgtgcc ggaagccatc
     3301 ctcggccgcc ttctggaggt cgtagtcgat cgtcaactgc acgcggcgcc cttcgaccgg
     3361 cttgatctcg tcgagcgtgt ccatctcgcg tcccacgctg ttgacgacca cgcgcttggc
     3421 gccgtcctga cccatcagga gcttgttgta gacgcgctcg atgccctgct ggccgacgat
     3481 ggctccgctc gcgatgccat cgccgatctg cgactcgttg gcctggctca cgtacccgaa
     3541 cagatgggcc gccagcgcgt ccgcggggta cttgcgggtc ggtacctgct cgaccatgac
     3601 gtcgggcaac tcgaagtcca gccggcgcgc gctgaccgcg gccacctgct gaagcgtcgc
     3661 gtcctccacc acgaggatcg ggcggtacga cggctcgcta agatggcgtt caatcgtctg
     3721 gaggacctgc ttgacgtcga ggcccgccac ctcggcgagc atgcgcccgg tgcgctcgag
     3781 atcctgcgag agttcgcgct cgatcgagat cgtgaacgac gggcggttct cgacgagcaa
     3841 agtcccgtcc ctgtcgtaga gcaacccgcg cggcgcccgc agggcgagcg tgcgctggtg
     3901 gttgttctcc gccatctcct cgtacttgtc gttctggacg atctggagaa cccagaaaca
     3961 gacccccagc gccgcaaacg ccacgatgac gccgacgtga agcacgacga gccgctcgcc
     4021 gatccgcctg cggttctcgc cctgggtcaa ctgcatagcg ccttcttcct accgtctcga
     4081 gcgtcgtccc gcgcgccgac gatcgagaat ccccggaatc gcctcgacga tcgcgaacga
     4141 aaccacgccc acgacggcat tgccgatcgc ctggctcgcc acggccttcc acggagacgg
     4201 aaacatccgc aggccgagcg cgacatacgc ccccatgaag agggccgcgt gtccggcggt
     4261 ggcgccgacg aacatcacga agcgaggcac cgccgacgtc agaatgaact gctggccgat
     4321 cgcgccggcc acgaacccga cgatcgcttt cgacaagccg ccgattccga gcactccact
     4381 cgacagcgca tcctggatga ggccggcgac gctgccagcc agcattcccc cgacaggccc
     4441 catgaccagc gcgaccgaaa ccacggcaac gagcaccaag tcgatggtcg tcgtgccgcc
     4501 gacgacgaga cgcgccagcg tcgtttgcaa cgccagggcc agcgccacgg cggcgagaac
     4561 gaccgcggcc ttcacggcgc ctcgacggcc ggtggcgtca gcacgaccag aatcgcctcg
     4621 agcgacgaaa actccaccgc agggcgaacg acgacgttgg tgaactcccc cgcctgccgc
     4681 tcgatcgatt ctatctgacc gatcacgaag cccttcgggt aaatcccttc gatgccggac
     4741 gacaccacgc ggtccccgac cacgatgtcg gccgcacccg ccagatggtt cagcttgagc
     4801 cgatcttccc ccgtgcccgt cacgaccccc tgggccctcg agcgctccac gagggcgccg
     4861 acagccgcgt tcctgtcgat gagcagctgt accttggacg ccctgacgcc gggcgtaatg
     4921 acccgcccga cgacgcccgc cggcgatagc acggccatgt cgggctgcat gccatccccg
     4981 gtccccttgt cgatcgtgat cgtccggaac tcgggactgg cgccgccggc aatcacggcg
     5041 gcggccgtcg tggacaactt ggtggtcgat cgcaggtcga gcagggcctg cagtgtcgcg
     5101 gactgctcgg cctgcgcgcg ttgctgctgc aggctgatct tgagggaggc aatctcctcg
     5161 cgcaggcgcg cgttctcgtc gctgacccct ctgagcgcga cgtagtcggt ccagcccgag
     5221 cgaacggcgc caatcaggcc gctggccgct cgctgaacct cggcaaagac accgacggtg
     5281 gcttcctcga gcacgggaac accccggttg gtattcacct gcgcggagat caggaggatg
     5341 tggccgacga tcacggccag gaagagccag cccgttcgct gtcggatatc gagaacggcc
     5401 at
//
LOCUS       DBNGSH010000177         3617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_522883, whole genome shotgun sequence.
ACCESSION   DBNGSH010000177 DBNGSH010000000
VERSION     DBNGSH010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_522883"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..958)
                     /locus_tag="AC16CY_04000"
     CDS             complement(185..958)
                     /locus_tag="AC16CY_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3352972.1"
                     /translation="MRNLSGPRWAATVALPAVAIPILAAQQTASSPLTTVSIRRNVSD
                     SPNANMQVRPGGRLFATNVTLRNMLRNIERLQESQLEGGPDWVATDRWDIAAVADTEV
                     SDVQAAALLKALLIERFKLKTHTETRDRPIYSLVVARADQALGPELRRSTVDCPTSQG
                     LCGDSSGPGTMRVVGRPILYTLRLFERASGRTVIDRTGLTGAYDFTLRWRPFDAPDVN
                     PDLPSLFTAVEEQLGLRLQPDRAPLDVVVIDSAQRPVED"
     gene            complement(1016..3265)
                     /locus_tag="AC16CY_04005"
     CDS             complement(1016..3265)
                     /locus_tag="AC16CY_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3352973.1"
                     /translation="MDSRSGLDGISPARIGHYRVLRKLGEGGMGVVYAAEDDRLGRQV
                     ALKVLRESSPDPDARARLVREARIAAGIAHPLICQVFELGEWNNQPFIAMELVDGEPL
                     AARLARGALAPGEALPIALFVVDALRVLHARGIVHRDLKPSNIFVTQAGVKVLDFGLA
                     RPVEASAADTATALTQAGTILGTPRYSAPEQLTGQAVDARADLFSAGVVLYEMLAGRA
                     PFQGANIGAQIHAVLYETPPVLTGSPAIAAIDRVLHRALAKDPGERYQTADAFGADLR
                     AVTPLVDSGARAEARPILRLAVLPFRLLKPDPETDYLGLSLADALASSLLGLESLVVR
                     SSLKSARYANTIPDLNALAADLAVDVVLTGSMLRVQDRMRVSAELVSVPAGDVWWSQT
                     THVAVDSVLDLHDDLARRVVASLPLTARDHAKPHAKAASAKAFDLYLRGMQLRSESSG
                     WRQAHSFFEQCLTLDPSFAPAWAERGRLERLIAKYEEPAKYREAESTLLHALELDPDE
                     GAAQYYYAQLEIDVGRLDHALARLLERVRQRRAEPHVYAALVHACRYAGLLEESVAAH
                     RQARRLDPTVSTSVHHTFYMQGDYARALDEVHHSNDPIEVRVLWAMGREAEALQVARR
                     EESRFAASPMLLAFSRGHRAAIEGRNDDVLSALQAFETIGHTDGEASFYVAGSYAKAG
                     HLDMAHALLTRAVDVGFLCLPAFERDIYLAPLRGTGAWRSLVERLTARHQRVVDEFVR
                     AGGRALLGQ"
BASE COUNT          624 a         1211 c         1231 g          551 t
ORIGIN      
        1 atccaccggg cacttcgtgc tctcgggtgg cggcgaagcg gagaggagcg gctggttcag
       61 cctgacgtgg gagaaggccg gcgccggctg gcggatcatc cacgatcatt cgagctaggg
      121 aactgcaagg gcctggcggc cacgcgccca ggagcgcgcg accgtcaacg cctgtccttc
      181 cgaatcagtc ttcgactggc cgctgggcgg aatcgatcac caccacatct aatggtgcgc
      241 ggtccggctg gaggcgcaga ccgagttgct cctccacagc ggtgaagagc gacggcagat
      301 ccgggttgac atccggcgca tcgaacggac gccagcgcag ggtgaagtcg tacgcgcctg
      361 tcaggcccgt acgatcgatg accgttcgtc cgctggcccg ctcgaacagc ctcagggtgt
      421 agagaatcgg gcgcccgacg acccgcatcg tccctggtcc cgagctgtca ccgcacagcc
      481 cctgtgaggt cgggcagtcc accgtactac gccgaagctc cggaccaagc gcctgatcgg
      541 cgcgtgcgac gaccagcgag tagatcggcc tgtcgcgagt ctcggtatgc gtcttcagct
      601 tgaatcgctc gatgagcagc gctttgagca aggccgccgc ctgcacgtcg gagacctcgg
      661 tatcggcaac ggcggcaatg tcccagcggt cggtggcgac ccagtcgggc ccgccctcga
      721 gctgagactc ctgaagcctc tcgatattgc gaagcatgtt gcgcagcgtg acgtttgtgg
      781 caaagaggcg gccgccaggc ctgacctgca tgttcgcgtt cggcgaatcc gacacgttgc
      841 gtcgaatgga aacggtcgtc aaaggcgacg atgccgtttg ttgcgccgcg aggatcggta
      901 tcgcgacggc gggcagcgcg accgtggcag cccatcgcgg tccgctgagg ttacgcatgc
      961 cagatactac gcggcgcgat tcaaacggtt aacaggacgg gatcgcagca gcctcctact
     1021 ggccgagcag cgcccgccca cctgcacgga cgaactcgtc gacgacacgc tggtgcctgg
     1081 ccgtaagacg ttccaccaac gatcgccagg cgccggtccc cctgaggggc gcaaggtaga
     1141 tatcgcgctc gaatgccggg aggcacagga agccgacgtc gaccgcacgc gtcagcagcg
     1201 catgggccat gtcgaggtgc ccggccttcg catacgatcc ggccacatag aaggacgcct
     1261 cgccgtcggt atgtccgatt gtctcgaacg cctgcaacgc cgacaggacg tcgtcattcc
     1321 ggccctcgat ggccgcgcgg tgtcccctgg agaacgcgag cagcatcggc gaggccgcaa
     1381 agcgtgactc ctcccggcgc gccacctgca gcgcttccgc ctcgcggccc atcgcccaca
     1441 ggacgcgcac ctcgataggg tcgttgctgt ggtgaacctc atcgagggcc cgggcgtagt
     1501 cgccctgcat gtagaacgtg tgatgcacgc tcgtcgagac ggtcggatcg agccgtcgtg
     1561 cctgccggtg agccgccact gactcctcga gaaggccggc gtaccggcag gcgtggacca
     1621 gcgcggcgta gacgtggggt tcggcgcgac gctggcggac gcgctcgagg agccgcgcca
     1681 gcgcgtgatc gaggcggccc acgtcgatct cgagctgcgc gtagtagtac tgcgctgccc
     1741 cttcgtcggg gtcgagctcg agcgcgtgca ggagcgtcga ttcggcctct ctgtacttcg
     1801 ccggctcctc gtacttcgcg atcagccgct cgaggcgccc gcgctcggcc caggcgggcg
     1861 cgaacgaggg gtcgagcgtc aggcactgct cgaaaaacga gtgcgcctgg cgccagccgc
     1921 tcgattcgct ccgcagctgc atgccgcgca ggtagagatc gaacgccttc gcgctcgcgg
     1981 ccttggcgtg aggctttgcg tggtccctgg ccgtcagcgg cagcgatgcc acaacccgcc
     2041 gggcgagatc gtcgtgcagg tcgagcaccg aatccaccgc gacgtgcgtc gtctgggacc
     2101 accacacgtc gcccgccgga accgacacga gctcggcgct gacgcgcatg cggtcctgga
     2161 cgcggagcat ggatcccgtg agcacgacgt ccaccgcgag gtcggccgcc agcgcgttca
     2221 ggtcgggaat cgtgttcgcg taccgtgccg acttgaggct ggagcggacc accagcgact
     2281 cgagaccgag cagcgagctc gcgagcgcgt cggcgagact caacccgaga tagtccgtct
     2341 cgggatccgg cttcaacagc cggaacggga ggacggcgag gcggaggatc ggccgggctt
     2401 ccgcgcgcgc gcccgaatcc acgagcggcg tcacggcccg aagatcggcg ccgaaggcat
     2461 cggccgtctg gtaccgctcc ccgggatcct tcgcgagagc ccgatgcagc acgcgatcga
     2521 tcgccgcgat cgccggcgag cccgtcagca ccggcggggt ctcgtagagc acggcatgga
     2581 tctgcgcgcc aatgttggcg ccctggaacg gcgctcgtcc ggcgagcatc tcgtacagca
     2641 cgacccccgc cgagaacaga tcggcgcgcg cgtccacagc ctggcccgtc aactgttcgg
     2701 gagcggagta tcgaggcgtg cccaggatcg tgcctgcctg cgtgagggcc gtcgccgtgt
     2761 cggcagcgga ggcctccacg gggcgggcga gtccgaagtc gagcactttc acgccggcct
     2821 gcgtcacgaa gatattcgag ggcttgagat cgcggtgaac gattccgcgg gcgtggagca
     2881 cgcggagcgc gtccacgacg aagagcgcga tgggcagcgc ctcgcccggc gcgagcgcac
     2941 cgcgcgccag ccgtgccgcc agcggctcgc cgtcaacgag ctccatcgcg atgaacggct
     3001 ggttgttcca ctctccgagc tcgaacacct ggcagatgag cgggtgcgca ataccggcgg
     3061 cgatgcgcgc ttcgcgtacc aaccgcgccc tggcgtcggg atcgggcgag ctctcccgga
     3121 gaactttgag agcgacctgc ctgcccagtc gatcgtcctc cgctgcgtag acgacgccca
     3181 taccgccttc accgagcttg cggaggacac ggtaatggcc gatgcgcgcc ggcgagatcc
     3241 cgtcgaggcc ggatcgcgag tccatacagt cagtcgatgc ttctcggggg aatgcagatc
     3301 agtgaataac caagatgatc cacgatgatc gaaacccgca cactcaagca cagccgtaga
     3361 cggcaagtca acgcggttcg gacgatataa tcacggcagc gccctgtcga gctcccaagc
     3421 tccatccccc cgcctgatgc cgggcagcgc tccggccgaa agcgatcaat atgaaacgac
     3481 atttctgttt cgctctcgtg ctgttggcgt gtgtcgtcgc aatgaactgc gattccaatc
     3541 cgacgaaccc ggacccgggc ggcggcggcg gggggggcgg tggcggcggc ggtggatctg
     3601 tcgcccaggc gaccgac
//
LOCUS       DBNGSH010000178         9115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2051241, whole genome shotgun sequence.
ACCESSION   DBNGSH010000178 DBNGSH010000000
VERSION     DBNGSH010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2051241"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..349
                     /locus_tag="AC16CY_04010"
     CDS             161..349
                     /locus_tag="AC16CY_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352974.1"
                     /translation="MPSMDARMGYLQGRMEEQSQMLQELRASVRHLDDRMSRQFTWTI
                     GIQITTLVAVLVALISRA"
     gene            complement(351..2054)
                     /locus_tag="AC16CY_04015"
     CDS             complement(351..2054)
                     /locus_tag="AC16CY_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3352975.1"
                     /translation="MSPVDWAIVAVYLVWIVWDGLRLTRRSKELEGYFLGGRSLPWWA
                     VGLSVMATQFSAITMVGTTGQGFVDGMRFLQFYYALPLAMIVLSVTLVPFFHRARVYT
                     AYEYLERRFDAKTRAFTSLLFLLSRSMSLGVVISAPAVVLAVVLGFDLTATVLLIAVP
                     TAVYTMFGGVQAVTWTDVKQMVLIVGALAAAVVVLVASLPDEVGVLQAMRIAGAAGRL
                     QTFDFSFDLTNPYTFWSGTIAALFLFCSYFGADQSQVQRYLTTRSVDEARESLLMSAY
                     WKIPLQALVLLVGVLMFLFFLYTPSPMLFNLVHDREMREGPRAADYQALESRFEAATA
                     SRSAAARVMSRAETAGDARSYEAARSEFRRQDAETSAVRGEAIDLVREATGDSGYNDV
                     NYVFPTFVTRFLPIGLPGLVVAAILAAAMSTISAELSALSSATVIDFYRRFVRHEASD
                     AHFLRVSRIATAFWGLVASAVAVWAAELGSLIEVVNRFGSFFYGSILGVFILAVGFSR
                     ATGTGAFVGLIAGMAAVAWTASATNVAFLWHNVIGAVACVVVGLVVSAAGAGSQRGAQ
                     A"
     gene            complement(2194..4698)
                     /locus_tag="AC16CY_04020"
     CDS             complement(2194..4698)
                     /locus_tag="AC16CY_04020"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR3352976.1"
                     /translation="MALLIGVPVTDTAAQTAVAETAAVHIGEGATGIVLDGDLSDAAW
                     AKAAPITAFVQREPLEGGQPTLRTEARVVYDAAAIYVAIRAFDPSPDEIVGFLTRRDA
                     DSSSDWVHVLIDSYHDRRTAYQFGVNPVGVKQDAYWFNDNNNDSNWDAVWDVVARRDA
                     DGWCAEFRIPFSQLRFSGAGDNRFGFAVVRNVARLNETATWPLLSRNASGFVSSFGEL
                     TGVTVGRASKRLELVPYAVGQLATTPVQQGNPLQTSPDPGAAVGVDLKYAVTPALTLN
                     ATVNPDFGQVEADPAVVNLSAFETFFDEKRPFFIEGSGTYQFQCRDCNLFYSRRIGRT
                     PRGAPLTADGEYSIQPLQSTILGASKLTGRVRNFSVGVLAAATQEEWADIAAGPLRRR
                     EVVEPGTFYSVSRARREFSDQSSLGFILTTTSRRLVDTVAFLPESAVTGGVDYDWRTG
                     QEWGLNGYWAGSSINGSAGAIERIQRSNVHSFQRPDADYVEVDPLAESLRGHSGNISF
                     GKIAGGWTRMNVSVGYKSPGFDVNDVGFQQRADEIPQNAWFQIRRETPGKYARSGYLN
                     FNQWSGFNFGGDRLSLGGNFNTHWQFQNLWSAGGGVNVNARRFDDRLTRGGPGGYVEP
                     YLSTWQYVGTNDRRAVSVNWNSGFGRGGEGRFFEFGPSVVVRPVSSLSAEVGLSYSNN
                     VDESQWVDEVRAADLTHYVFGRLSQETSSMTFRVNYTMTPDLSLQVYAQPFVSTGRYT
                     DYKELVNGRSVNYDERYAPYDYARNADFKVLSFRMTNVLRWEYKPGSTLFVVWQQGRE
                     GSGTPRPFAFQNDFGDAFSTPAANVLLVKLAYWLNP"
     gene            complement(4828..6255)
                     /locus_tag="AC16CY_04025"
     CDS             complement(4828..6255)
                     /locus_tag="AC16CY_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIR3352977.1"
                     /translation="MRRVVVGILVMLSMAASITVSAQDVGFGSAWPDLTPAERDEVQK
                     LGEDFKTFLGRAKSEMLFVRSATQFAESNGFRKWDAASSAAALKPGSRWYAINRDRTI
                     VLIVIGTEPLENGMRIVNTHIDSPRIEFKTRPFRESQQAVLIDTQVHGGLKNYQWVNV
                     PLAITGRVDKSDGTTVWIDVGNLPDDPVLLITDLAPHVDRDFRGRTQTEVIRTEELDP
                     VVGTLPPDAAAGQRTATDRVLALLHEKYGVTARDFLSADLQIVPATMPRDVGLDRALI
                     GAYGQDDRSTAYISLRAIADVKTPRYTSVAYAVNNEEVNSWNTGVNSEWFSTLVSELL
                     AAQKGREFSDLSLRRAYARTEVLVSDTTTAVNPLFPQPQNPNLASRLGYGLVVKEYGA
                     GREANSEFFAKMRGLFDRGRVRWQIQAYDAGYGGSTIAAWFAQQNMDVIDVGIGIVSM
                     HSPFEVSSKVDLWHLRRGFLAFFGN"
     gene            complement(6313..8181)
                     /locus_tag="AC16CY_04030"
     CDS             complement(6313..8181)
                     /locus_tag="AC16CY_04030"
                     /inference="COORDINATES: protein motif:HMM:NF036516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR3352978.1"
                     /translation="MRPRALGAALLLLALAPLAALAQTVSYRLSFPEAAHHVMHVEAT
                     FRSLPPGPLELRMSRSSPGRYAAHDFAKNVFDVRITDASGKALAVTRPNPHQWNVTAH
                     GEEVRVSYKVFGDRLDGTYLAIDSSHAHINMPAAIMWARGLERTPATLRVERPADTSW
                     RVATQLPSSGDGLTFTAPNLHYLMDSPIELGNLAMRTFTVTDEQRTPVFRLAVHHTGT
                     DADIDGLARDVETITREARHIFREYPAFEGNTYTFIADYVPWAINDGMEHRNSTVLTS
                     PSTIRGARLELLDTIAHEFFHAWNVERIRPKSLEPFNFEDANISGELWLAEGFNSYYA
                     PLIMKRTGLTTLRDFVLEMGRVIDTVVTSPGRQVRSAVEMSQFAPFTDEAAAIDRTNF
                     SNTVISYYTWGSAIALGLDLTLRERTDGKVTLDDFMRQLWNRFGKPGAREAGYVATPY
                     THDDLRRTLGEVAADQAFADDFFARYVEGRDVVDYAPLVARAGLVLRSRKDRAFAGAL
                     WLQGDQAGVRILGDVPLGSPAYEAGLEREDVIVSLGNTRVSQAADVERLVVARKPGDP
                     LPVVFERRGARVTSVIRLGPDPRVELVTTEEAGQTLTDAQRRFRAVWLNSAAGNSF"
     gene            complement(8282..8863)
                     /locus_tag="AC16CY_04035"
     CDS             complement(8282..8863)
                     /locus_tag="AC16CY_04035"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: protein motif:HMM:NF024952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HIR3352979.1"
                     /translation="MSQLVAIDVAVLLPQDVSGRAIELSAMLAGGDPQALRLGAGCLP
                     HITLTQQFVGAVSLDACLDAVGVVLQGVAPLHMSVTGVGRGNTTVWMSVERSPALLDL
                     HRRLMDALETFEQTDGAADAFVGNARPKDIEWVRSYRRASSGKAFTPHITLGHSTQTT
                     PLVEPLTFEAATIAACQLGRFCSCGRVLRRWTL"
     gene            8894..9112
                     /locus_tag="AC16CY_04040"
     CDS             8894..9112
                     /locus_tag="AC16CY_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352980.1"
                     /translation="MIDFLRALRAHAAYKQRLVSFAPALIVSELFFKFHSFTLECLAF
                     LATWLVFDVVAEWVAGKPVSTGRAVERT"
BASE COUNT         1596 a         3012 c         3011 g         1496 t
ORIGIN      
        1 agaatctcca gcggtcccgc cgctgggaag ttgcagaatt tgcaacactt cgcggcccct
       61 gtcgtccctt ccgtgcaaag ggcgtcggcc tccgtcttgc tttgatctgt gtgccttgat
      121 caaatcgacc agacgacgat acgctgaggg agacgacgac gtgcccagta tggacgcgcg
      181 aatgggttat ctccagggac gaatggagga gcagtcgcag atgctccagg agttgcgggc
      241 atccgttcgt catctcgacg acaggatgtc gaggcagttc acctggacga tcggcattca
      301 gatcacgacg ctcgtggccg tgctcgtcgc gctgatctcg cgcgcctgat tcacgcctga
      361 gcgccgcgtt gcgatccggc gccggccgcg ctgaccacca atcccaccac gacgcacgcc
      421 acggcgccga tgacgttgtg ccagaggaac gccacgttgg tggcgctcgc cgtccaggcc
      481 acggcagcca tcccggcgat caggccgacg aacgcacctg tgcctgtcgc ccgcgaaaag
      541 ccgacggcca ggatgaacac gccgaggatc gagccgtaga agaaggaccc gaatcggttg
      601 accacctcga tgagtgaccc gagctcggcc gcccataccg cgacggcgct cgcgacgagt
      661 ccccagaacg ccgtcgcgat tcgcgacacc cgcaggaagt gcgcgtcgct cgcctcgtgg
      721 cgaacgaagc ggcggtagaa gtcgatcacc gtcgcgctcg agagcgccga gagctcggcc
      781 gaaatcgtgg acatcgccgc cgcgagaatc gccgcgacga cgaggcctgg aaggccgatc
      841 ggcaggaagc gggtcacgaa cgtcggaaag acgtagttca cgtcgttgta gccggaatcg
      901 cccgtcgcct cgcgcacgag gtcgatcgcc tcgccccgca ccgcgctcgt ctcggcgtcc
      961 tgcctgcgga actcgctccg cgcggcctcg tagctgcgtg cgtcgccggc cgtctcggcg
     1021 cgtgacatga ccctggcggc ggcggatcgg gacgccgtgg cggcttcgaa ccgcgattcg
     1081 agcgcctgat agtccgcggc gcgcggcccc tcgcgcatct cgcgatcgtg gacgagattg
     1141 aacagcatgg gcgacggcgt gtagaggaag aagaggaaca tcagcacgcc gacgagcagc
     1201 accagcgcct gcagcgggat tttccagtag gcgctcatca gcagcgactc gcgggcctca
     1261 tcgaccgagc gtgtcgtcag atagcgctgg acctggctct ggtcggcgcc gaagtacgag
     1321 cagaacagga acagcgcggc aatcgtgccg gaccagaagg tgtacggatt cgtcaggtcg
     1381 aaggagaaat cgaacgtctg cagacggcca gccgcgcccg cgatccgcat cgcctgcagc
     1441 acgccgacct cgtcgggcag gctcgcgacg agcacgacca ccgcggcggc cagcgccccc
     1501 acgatcagca ccatctgctt gacgtcggtc cacgtcaccg cctgcacgcc gccgaacatc
     1561 gtgtacaccg ccgtgggcac ggcgatcagc agcacggtgg ccgtcaggtc gaagccgagc
     1621 acgacggcca gcaccacggc cggcgccgag atgacgacgc cgagcgacat gctgcgcgag
     1681 agcaggaaca gcaggctggt gaacgcgcgc gtcttcgcgt cgaagcggcg ctcgagatat
     1741 tcgtaggcgg tgtagaccct ggcgcggtga aagaagggaa ccagcgtgac cgagagcacg
     1801 atcatcgcga gcggcagcgc gtagtagaac tgcaggaagc gcatgccgtc cacgaagccc
     1861 tgaccggtgg tgccgaccat cgtgatggcg ctgaactgcg tcgccatcac cgacagcccg
     1921 accgcccacc acggcaggct ccgcccgccg aggaaatacc cctccagttc cttgcttctc
     1981 ctggtgaggc ggagaccgtc ccacacgatc cacaccaggt agacggcaac gatcgcccag
     2041 tcgacggggc tcacgaaaag atttcctgga agctgtagag cgccgccaga acggcggccc
     2101 agacgatgac gacgcggacg tagtggcgct cgacggaggt cacggctcga atcgcatgga
     2161 gtatagcggc tgccggctac ccagctgcgc agctcaggga ttcagccaat atgcgagctt
     2221 gacgagcagc acgttcgcgg cgggcgtcga gaaggcgtcc ccgaagtcgt tctggaacgc
     2281 gaacggtctt ggggtgccgc tgccctcgcg cccctgctgc cacaccacga agagcgtcga
     2341 tcccggtttg tattcccagc ggagcacgtt ggtcatgcgg aaggacagca ccttgaaatc
     2401 ggcgttgcgc gcgtagtcgt acggcgcgta tcgctcgtcg tagttcactg agcggccgtt
     2461 cacgagctcc ttgtagtcgg tgtagcgtcc ggtgctgaca aacggttgcg cgtacacctg
     2521 cagcgagagg tccggcgtca tcgtgtagtt gacgcggaag gtcatggagc tggtttcctg
     2581 cgacaggcgc ccgaagacgt aatgcgtcag gtccgctgcc ctcacctcgt ctacccattg
     2641 cgactcgtcc acgttgttgc tgtagctgag gccgacctcg gccgaaagcg acgacaccgg
     2701 ccgcacgaca accgagggcc cgaactcgaa gaagcgcccc tccccgcccc ggccgaagcc
     2761 cgagttccag ttcacggata cggcgcgtcg atcgttggtg cccacgtact gccaagtcga
     2821 gaggtacggc tcgacgtagc ctcccgggcc gccgcgagtc agccgatcgt cgaaccttct
     2881 ggcattgacg ttcacgccgc caccggcgct ccataggttc tggaattgcc agtgcgtgtt
     2941 gaagttgccg cccagcgaca gccgatcgcc gccgaagttg aaccccgacc actggttgaa
     3001 gttgagatag ccgcttcgcg cgtacttgcc gggcgtctcc cgcctgatct gaaaccacgc
     3061 gttctgcgga atctcgtccg cgcgctgctg aaaacccacg tcgttcacat cgaatcccgg
     3121 cgacttgtat ccgacgctga cgttcatgcg cgtccagccg cccgcgatct tgccgaacga
     3181 gatgttgccc gagtggccgc gcagcgactc ggccagcgga tcgacctcga cgtagtcggc
     3241 gtcgggccgc tgaaagctgt gcacgttgct tcgctggatc cgctcgatgg caccggcgct
     3301 tccgttgatg ctgctcccgg cccagtagcc gttcagcccc cactcctggc ccgtccgcca
     3361 atcgtagtcg acgcccccgg tcacggcgct ctcggggaga aaggcgaccg tgtccacgag
     3421 gcgacggctc gtcgtggtca gaatgaagcc gagcgacgac tgatcggaga actcgcggcg
     3481 cgcgcgcgac acggaataga acgtccccgg ttccacgacc tcgcggcggc gcagaggacc
     3541 cgcggcgatg tccgcccact cctcctgcgt ggcggccgcc agcacgccga ccgagaaatt
     3601 gcggacgcgc cccgtcagct tgctcgctcc cagaatcgtc gattgaagcg gctgaatcga
     3661 gtactcgccg tcggccgtca aaggggcacc acgcggcgtg cggccgatgc ggcgagagta
     3721 gaacagattg caatcgcggc actggaactg atacgtgccc gagccttcga tgaagaacgg
     3781 acgcttctcg tcgaagaacg tctcgaacgc gctgaggttc acgacggccg gatcggcttc
     3841 cacctgaccg aagtccgggt tgacggtcgc gttcagcgtg agcgcgggcg tcacggcgta
     3901 tttcaaatcg acgcccaccg cggcgcccgg atcgggcgac gtctgcagag ggttgccctg
     3961 ctgcacgggc gtggtggcga gctgcccgac ggcgtacgga accagctcga gccgcttcga
     4021 ggcgcggccc acggtcacgc ccgtgagctc tccgaaggag gacacgaatc cgctcgcgtt
     4081 cctcgacagc agcggccacg tcgcggtctc gttcaggcgc gcgacgttgc gaaccacggc
     4141 aaagccgaac cggttgtcgc ccgctccgct gaagcggagc tgggagaacg ggatccggaa
     4201 ctcggcgcac cacccgtccg cgtcgcggcg ggcgacgaca tcccacacgg cgtcccagtt
     4261 gctgtcgttg ttgttgtcgt tgaaccagta ggcatcctgc ttcacgccga ccgggttgac
     4321 gccgaactgg tacgcggtac ggcggtcgtg gtaggagtcg atcaggacgt ggacccagtc
     4381 ggaggacgag tcggcgtcgc gacgggtcag aaagccgacg atctcgtcgg gactggggtc
     4441 gaaggcccgg atggccacgt agatggccgc cgcgtcgtag acgacccgtg cctcggtgcg
     4501 gagggtcggc tgcccgcctt cgagcggttc gcgctggaca aacgccgtaa tgggcgcggc
     4561 cttcgcccat gcagcatcgc tcagatcccc gtccaggaca atgccggtgg caccctcgcc
     4621 gatgtgtaca gccgctgtct cggcaacagc ggtctgtgcg gccgtgtccg ttaccgggac
     4681 accaatcagc aacgccacca gggcgctgga tacacatcga ctactcacgc cggtccacag
     4741 acgctttaga cgcaagtttc ctgcgtttgg ttacatgcgc ggatgaaggg gcatgcctga
     4801 ggtagccgca gaccgaagcc cgagtgatca gttcccgaaa aacgcgagaa accctcggcg
     4861 caggtgccag agatcgacct ttgacgacac ctcgaacggc gagtgcatgc tgacgatgcc
     4921 gatgcccacg tcgatgacat ccatgttctg ttgcgcgaac caggcggcaa tcgtgctgcc
     4981 gccgtagccg gcgtcgtagg cctggatctg ccatctgact ctgccgcgat cgaacaggcc
     5041 gcgcatcttt gcgaagaact ccgagttggc ttcgcggccc gcgccgtact cctttacgac
     5101 caggccgtac ccgagacgcg acgccaggtt cgggttctgc ggctgcggaa acagcgggtt
     5161 gaccgccgtg gtggtgtccg acacgagcac ctcggtcctg gcataggcgc gccgcagcga
     5221 cagatcgctg aactcccggc ccttctgcgc cgccagcagc tccgagacga gcgtgctgaa
     5281 ccactccgag ttcacgcccg tgttccagga gttcacttcc tcgttgttca cggcgtaggc
     5341 gaccgacgtg taacggggcg tcttgacgtc cgcgatcgcg cgaaggctga tgtaggccgt
     5401 ggagcggtcg tcctgcccgt acgccccgat caaggcgcga tcgagcccga cgtcccgcgg
     5461 catcgtcgcg ggaacgatct ggagatcggc agacaggaaa tcgcgcgccg tgacgccgta
     5521 cttctcgtgc agcagcgcga gcacgcggtc tgtggccgtg cgctgaccgg cggcggcatc
     5581 gggaggaagc gtaccgacga ccgggtcgag ctcctccgtg cgaatgacct cggtctgcgt
     5641 gcgtccgcgg aagtcgcggt ccacgtgggg cgccagatcg gtgatgagca gcacgggatc
     5701 gtcgggaagg ttccccacgt cgatccacac ggtcgtcccg tcggacttgt ccacgcggcc
     5761 cgtgatggca agcggcacgt tgacccactg gtaattcttg aggccgccgt gcacctgcgt
     5821 gtcgatcagc acggcctgct ggctttcccg gaacggacgc gtcttgaact cgatgcgcgg
     5881 ggagtcgatg tgcgtgttga cgatgcgcat gccgttctcg agcggctcgg tgccgatgac
     5941 gatcaagacg atggtgcggt cgcgattgat tgcataccac cgcgagcccg gcttgagcgc
     6001 ggcggcggac gaggcggcat cccatttacg gaacccgttg ctctcggcga actgcgtggc
     6061 gcttcgcacg aagagcatct cggacttcgc ccgcccgagg aaggtcttga agtcttcgcc
     6121 gagcttctgc acctcgtcgc gctccgcggg tgtcagatcg ggccatgcgc tcccgaagcc
     6181 gacgtcctgc gcgctcaccg tgatcgacgc ggccatgctg agcatcacga ggattccgac
     6241 cacgactctt ctcatcacgc tctctccccg cttcatgacg tagaggcggg tcttgagacc
     6301 cgcctaggct ttctaaaacg agtttcccgc cgccgagttc agccagactg cgcgaaaacg
     6361 cctctgcgcg tcggtcagcg tctgcccggc ctcttccgtc gtgaccagct cgactcgcgg
     6421 atcgggtccc aagcggatca cgctcgtcac ccgggcgccg cgccgttcga acacgaccgg
     6481 cagcggatcg cccggcttgc gggcaacgac cagccgctcg acgtcggcgg cctggctcac
     6541 gcgggtgttt ccgagcgaca cgatcacgtc ctcgcgctcg agcccggcct cgtaggccgg
     6601 ggaacccagc ggcacgtcgc ccagaatgcg gacccccgcc tgatcgccct gcagccagag
     6661 cgcgccggca aacgcgcggt ctttcctcga tctcaacacg aggccggctc gcgcgacgag
     6721 cggcgcgtag tccaccacgt cgcggccctc gacgtagcgc gcaaagaaat cgtcggcaaa
     6781 cgcctggtcg gccgcgacct cgccgagagt ccgcctcaga tcgtcgtggg tgtacggcgt
     6841 tgccacgtat ccggcctcgc gcgcgccggg ctttccgaag cggttccaga gctggcgcat
     6901 gaagtcgtcg agcgtgacct tgccatccgt gcgctcccgg agcgtcaggt cgaggccgag
     6961 ggcgatggcg gacccccagg tgtaatacga gatgaccgtg ttcgagaagt tggtgcgatc
     7021 gatcgcggcc gcctcgtcgg tgaacggcgc gaactggctc atctcgacgg cggagcggac
     7081 ctgacggccc ggactcgtca ccaccgtgtc gatgacgcgg cccatctcca gcacgaagtc
     7141 ccggagcgtg gtcagcccgg ttcgcttcat gatgagcggg gcgtagtagc tgttgaagcc
     7201 ttcggccagc cagagctcgc ccgagatgtt cgcgtcttcg aaattgaacg gctcgagcga
     7261 cttcggccgg atccgctcca cgttccaggc gtggaagaat tcgtgggcga tcgtgtccag
     7321 cagctcgagc ctggcgccgc ggatcgtcga gggcgacgtc agcacggtgc tgttgcgatg
     7381 ctccatgccg tcgttgatcg cccagggcac gtagtcggcg atgaacgtat acgtgttgcc
     7441 ctcgaaggcc gggtattcac ggaagatgtg cctggcttcc ctggtgatcg tctcgacgtc
     7501 gcgggccagg ccgtcgatgt cagcgtcggt gccggtatgg tgcaccgcga ggcggaacac
     7561 gggcgtccgc tgctcgtcgg tcacggtgaa ggtgcgcatc gcgaggttgc cgagctcgat
     7621 ggggctgtcc atcaggtagt gcaggttggg agcggtgaag gtcaggccgt cgccgctcga
     7681 aggaagctgc gtggcaaccc gccacgacgt gtcggccggg cgctcgaccc gcagcgtggc
     7741 aggagtccgc tcgaggcccc gtgcccacat gatcgccgca ggcatgttga tgtgagcgtg
     7801 cgagctgtcg atcgccagat acgttccgtc cagacggtcg ccaaacacct tgtagctgac
     7861 tcgcacttct tcgccgtggg ccgtcacgtt ccattggtgc ggattcggac gagtgacggc
     7921 aagagccttg cctgaggcgt ccgtgatgcg gacgtcgaag acgttcttcg cgaagtcgtg
     7981 cgcggcgtat cgccccggcg aggaccggct catgcgcaac tcgagcggcc ccggcggcag
     8041 gctcctgaac gtggcctcga cgtgcatcac gtggtgcgcc gcctcgggaa acgacaggcg
     8101 ataggacacc gtctgcgcga gcgcggcgag cggcgcgaga gcgagcagca ggagcgcggc
     8161 gccgagggca cgagggcgca tgcacacgga tgctacaccg gagttcatgt gcatcgtagg
     8221 ggcccgtctt tcctggcctg agcaaagtcg aaggcagacc ggcccaggcc agagggctac
     8281 gtcacaacgt ccagcgccgc agcacgcggc cgcaggagca gaaccttccg agctggcagg
     8341 cagcgatcgt agccgcctcg aacgtcaggg gttcgacaag gggcgtcgtc tgggtggagt
     8401 gtccaagcgt gatgtgaggc gtgaacgcct tgccgctcga tgcgcgccga tagctgcgga
     8461 cccattcgat gtcctttggt ctggcgttgc cgacaaacgc gtccgccgcg ccgtcggtct
     8521 gctcgaacgt ctcgagcgcg tccatcaggc ggcgatggag gtcgagcaaa gcaggcgacc
     8581 gctcgacgct catccagacg gtcgtgttgc cccttccaac gccagtgacg ctcatgtgga
     8641 gcggcgcaac accttgcaga acgacaccga ccgcgtcgag gcaggcgtcg aggctgacgg
     8701 cgcccacgaa ctgctgagtc agcgtgatat gcggcagaca gccggcgcca agccgcaggg
     8761 cctgagggtc tccgccggcc aacatcgcgc tgagctcgat ggcccgcccg gacacatcct
     8821 gaggaaggag caccgcgacg tcgatggcga ccagttgaga caagcggtcc gcgatcgtac
     8881 tattgccgtc gccatgatcg actttctccg cgctctccgc gcccacgccg cctacaagca
     8941 gcggctggtg agtttcgcgc cggccctgat cgtcagcgag ttgttcttca agttccacag
     9001 cttcacgctg gagtgcctcg cgttcctcgc cacctggctc gtcttcgacg tggtggccga
     9061 gtgggtcgct ggaaagccgg tcagcacagg ccgcgcagtc gagcgcacct gattc
//
LOCUS       DBNGSH010000179         4626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2051374, whole genome shotgun sequence.
ACCESSION   DBNGSH010000179 DBNGSH010000000
VERSION     DBNGSH010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2051374"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="AC16CY_04045"
     CDS             complement(<1..1029)
                     /locus_tag="AC16CY_04045"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3352981.1"
                     /translation="MPRIFISYRRDDVAGDAGRLADHLQRRFGRDQVFLDIDTIDPGK
                     DFVQELHASLQETAAVLVVIGPRWTTASDAAGARRLDNAKDFVRLEVEAALERTIPVV
                     PVLVQGAAMPRAEELPAALASLATRQAAVLDHAEFHDDAERLCDRLERMIAGRKSPWQ
                     AARRWWPAAAVAAVLAIGIGYLALRSPDKGSSTATTGSAAPDANTGGAAAAAGTEGAK
                     ESAGTESVKGTESVKESGQSRRVDELLAAAEAQRGRNQFTEAMATLAGARELAPASGA
                     VRRAQEDVAMEWIRNARVEGGKSSFGDSIKPALAVVDTSLPSATGTRRADLLAHTGWA
                     TFLLWRDGD"
     gene            complement(1036..1917)
                     /locus_tag="AC16CY_04050"
     CDS             complement(1036..1917)
                     /locus_tag="AC16CY_04050"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015495788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIR3352982.1"
                     /translation="MKDNRLLDGRAVRDRILQDVAEQVRSAAATHAIGRLVSISIGEH
                     KEVAVYVRGQANAARKVGLRFEEQTWPAGLTQEECKARLVAMNDDADVLGVILQRPVP
                     AHIHGRSLASAIHPLKDLEGMNPASIGNIVYNDLALAPCTAAASVELIKATGLNLHGL
                     EVVMVGHSEIVGKPAAFMLMAQGATVTVCHHLTRSVAAHSRRADAVLVAVGKPKFLRA
                     DMVKPGAAVIDIGINQITGPNGETMIVGDVDTDSVKEVASWVTPVPGGVGPVTVAMFM
                     RNAIAAHARQFAAGWLR"
     gene            1962..2318
                     /locus_tag="AC16CY_04055"
     CDS             1962..2318
                     /locus_tag="AC16CY_04055"
                     /inference="COORDINATES: protein motif:HMM:NF016854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR3352983.1"
                     /translation="MPRPRTRGRLPSLRVSDSFKSFVLDQLSDLDGVMPKAMFGGIGL
                     YHRDTFFGILARDTLYLKVDDTTRADYERLGMKPFKPYPGRPTTMRYYEVPAGVLESS
                     AELVSWARKAVAVARR"
     gene            2359..3582
                     /locus_tag="AC16CY_04060"
     CDS             2359..3582
                     /locus_tag="AC16CY_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3352984.1"
                     /translation="MRKHVLILIAFVLTTTGQAQRPAADVTQWRGAARDGVITGLTAP
                     QAWPEMLTQRWKVDIGTGYATPLVVGNRVYQFSRLGENETMTALDADSGKVVWQAGYP
                     ATFTMNPATKQHGPGPKSTPIFSGGRLYSIGMTGTVTAWDAASGKQVWQKPGAGPDML
                     YTTHAFSPLVDGGRVIFHVGGNDKGALTAFDLNTGDVRWSWTGDGPGYASPIVAELGG
                     TRQIITPTQTKIVGVDASTGALLWERPLIHQFTSNCITPIVSGQTVIVAGTGPLFAFA
                     VKRGAQWTTEQLWENAELPLRYTTPVVSGDMLFGLSGRNAGQYYAMDARSGKTLWTSE
                     GRQAAHASVARAGDLLISLESDGELVVARNSQTAFEPIRRYKVAATETWAQPAISGNR
                     AFIKDVTSLALWTWN"
     gene            3721..4014
                     /locus_tag="AC16CY_04065"
     CDS             3721..4014
                     /locus_tag="AC16CY_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352985.1"
                     /translation="MDFTADGAQLTGAITRTSPPGQKPVAVKGKVDKDTITFTVLSPD
                     GKRTITFTGKVMGDEIVFVREAKGDPGAGSNQGGGFGIYGLNGPKTVTVKRVK"
     gene            4122..4385
                     /locus_tag="AC16CY_04070"
     CDS             4122..4385
                     /locus_tag="AC16CY_04070"
                     /inference="COORDINATES: protein motif:HMM:NF023174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 domain-containing protein"
                     /protein_id="HIR3352986.1"
                     /translation="MVIESFRGGDPVPIYQRFRDRGRLMPNGVEYRGSWVTEDLSRCF
                     QVMECEDRGLLDEWMANWSDITDFEVVPVLTSAQAREVVGPRL"
     gene            4392..>4626
                     /locus_tag="AC16CY_04075"
     CDS             4392..>4626
                     /locus_tag="AC16CY_04075"
                     /inference="COORDINATES: protein motif:HMM:NF046346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3352987.1"
                     /translation="MRQRPSISGISPFFIVADIPASLAFYRDVLGFEITFRGPTPEDE
                     FFGIVRRDGATIMFKALGVIVDGKEVLVEPVPNY"
BASE COUNT          782 a         1515 c         1522 g          807 t
ORIGIN      
        1 gtcgccgtcg cgccacagca ggaacgtggc ccatccggtg tgagccagca ggtcggcccg
       61 acgagtcccg gtggcggagg gaagcgaggt gtccaccact gcaagcgccg gcttgatgga
      121 gtcgccgaac gacgacttgc cgccctcgac ccgggcgttg cggatccact ccatcgccac
      181 gtcctcttgc gctcgtcgaa cggctcccga cgccggcgcc agttcccggg cgccggccag
      241 cgtcgccatc gcctcggtga actggttccg gccgcgctgt gcctccgccg cggccagcag
      301 ttcgtctacg cgtcgcgact ggccggactc cttgacgctt tcggtcccct tgacgctttc
      361 ggtcccggcg gactccttgg cgccttcggt cccggcggcc gccgcggcgc cgccggtgtt
      421 ggcatccggc gctgccgatc cggtcgtcgc tgttgacgat cccttgtccg gcgaacgcag
      481 agcaaggtag ccgattccga tcgccaggac ggccgccacc gccgccgccg gccaccacct
      541 cctcgcggcc tgccagggcg acttccggcc cgcaatcatc cgctccaggc gatcgcacag
      601 gcgctcggcg tcgtcgtgaa actccgcgtg gtccagcacg gccgcttgcc gcgtggcaag
      661 cgacgcgaga gccgccggca gttcttcggc tcgaggcatc gccgctccct gaacgagcac
      721 gggcacgacg gggatggtcc gctccagcgc cgcctcgact tcgagccgga cgaagtcctt
      781 cgcgttgtcg agcctgcgcg cgccggctgc gtcgctggcc gtcgtccatc gcggcccgat
      841 cacgaccagg accgcagcgg tctcctgaag ggatgcgtgc agttcctgca cgaagtcttt
      901 gcccggatcg atcgtgtcga tgtccagaaa cacctggtcc ctgccgaagc ggcgctgcag
      961 gtggtcggcc aggcggccag catcgcccgc cacatcgtct cttcgatagc tgatgaagat
     1021 tcggggcacc ggtcctcacc tgagccagcc ggctgcgaac tgcctggcgt gagcggcaat
     1081 ggcgttccgc atgaacatcg cgacggtcac cgggcccact cccccaggga ccggagtgac
     1141 ccagctcgcc acttccttca cgctgtcggt gtccacatcg cccacgatca tcgtctcgcc
     1201 gttcggcccc gtgatctggt tgatgccgat gtcgatgacg gccgcgccgg gcttgaccat
     1261 gtcggcgcgc aggaacttcg gcttgccgac cgcgacgagc acggcatcgg cgcggcgtga
     1321 atgggcggcc acggatcgcg tcaggtggtg gcacacggtt accgtcgccc cctgcgccat
     1381 cagcatgaac gcagccggct tcccgacgat ctcggagtgg ccgaccatga cgacctccag
     1441 gccgtgcagg ttgagcccgg tcgccttgat cagctcgacc gacgccgcgg cagtacaggg
     1501 cgccagcgcc aggtcgttgt agacaatgtt gccgatcgag gccggattca tcccctcgag
     1561 gtccttgagc ggatggatcg cggatgcgag cgagcggccg tgaatatgcg ccggcacagg
     1621 gcgctgcagg atgacgccga ggacgtcggc atcgtcgttc atggcgacga gcctggcctt
     1681 gcattcttcc tgggtgagcc cggcgggcca ggtctgttcc tcgaagcgaa ggcccacctt
     1741 tctcgccgcg ttcgcctggc cgcgcacgta gaccgccact tccttgtgct cgccgatgct
     1801 gatggagacg aggcgtccga tcgcgtgggt cgccgccgcg ctgcgcacct gctcggcgac
     1861 gtcctggaga atgcggtcgc ggacagctcg accatcgaga agccggttat ccttcatggc
     1921 gcggttgata cactggcccg agtgtagagt tcctgctgca tatgccgcga ccacgcacgc
     1981 gcgggcgact tccgtccttg agggtaagcg acagcttcaa gtcgttcgtc ctcgatcagc
     2041 tgtcagatct ggatggcgtc atgccgaaag cgatgttcgg cggcatcggc ctctatcatc
     2101 gtgacacgtt cttcggaatt ctcgcgcgtg acaccttgta tctgaaggtc gacgacacca
     2161 cccgcgccga ctacgagcgc cttgggatga agccgttcaa accgtatccg ggccgcccga
     2221 cgaccatgcg ctattacgaa gtgccggccg gtgtgctcga aagctccgcc gagctggtgt
     2281 cgtgggcgcg caaggcagtc gccgtggcgc gccggtgacg tgcagcgtag caccctttcg
     2341 cagttagaat tctgccctat gcgtaaacac gtccttatcc tgatcgcttt tgtactgacc
     2401 acgacgggac aggcgcagcg cccggctgcc gacgtgacac agtggcgcgg cgccgcgcgc
     2461 gacggcgtca tcacgggcct gaccgcgccg caggcgtggc ccgaaatgct cacgcaaagg
     2521 tggaaggttg acatcggcac gggctacgcc accccgctcg tcgtcggcaa ccgcgtgtat
     2581 cagttctccc gccttggcga gaacgaaacg atgacggccc tcgacgcgga cagcggcaag
     2641 gtggtctggc aggcgggtta tcccgcgacg ttcacgatga acccggcaac caagcagcac
     2701 ggccctggac ccaaatcgac gccgatcttt tccggcgggc gcctgtattc gatcgggatg
     2761 accgggacgg tcaccgcctg ggacgcggcg tcgggcaagc aggtgtggca gaagcccggt
     2821 gcggggcccg acatgctcta cacgacgcac gccttctcgc cgctcgttga cggcggccgt
     2881 gtgatctttc acgtgggcgg caacgacaag ggtgcgctga ccgcgttcga tctcaacacg
     2941 ggcgatgtgc ggtggagctg gaccggcgac ggccctggct acgcgtcgcc gatcgtcgcc
     3001 gaactgggcg gcactcgaca gatcatcacg ccgacgcaga cgaagatcgt cggcgtcgat
     3061 gcctcgacgg gcgcgcttct ctgggagcgt ccgctcatcc atcagttcac gtccaactgc
     3121 atcaccccga ttgtgagcgg gcagacggtc atcgtcgcag gcaccgggcc gctcttcgcg
     3181 ttcgccgtca agcgcggcgc gcagtggacg accgagcaat tgtgggagaa cgccgaactg
     3241 ccgttgcgct acacgacgcc cgtcgtatcg ggcgacatgc tcttcggtct ctcgggtcgc
     3301 aacgccggac agtactacgc gatggatgcc aggagcggca agacgctctg gacgtccgaa
     3361 gggcggcagg ccgcgcacgc gtcggttgcg cgggcgggcg atctgctgat cagtctcgaa
     3421 agcgacggcg agctggtcgt ggcgcgcaac agtcagacgg cgttcgagcc gatccggcgc
     3481 tacaaggtcg cggccaccga gacgtgggca cagcccgcga tttcgggaaa ccgcgccttc
     3541 atcaaggacg tgacgtcgct ggcgctgtgg acgtggaatt gaagggagat tcccgtgaag
     3601 gctgcaaccc ttgcgtgttc ggtcgtggtc gccgcgtcag cgactctcct cgcgcagtca
     3661 cccatcaatg gtaagtggac gacagaagct gttggaatcg gcggggccga tacggtcgtc
     3721 atggatttca cggcggacgg cgctcaacta acgggcgcga tcacacgcac cagcccacca
     3781 ggtcagaaac cagtcgcggt caagggcaag gtcgacaagg acacgatcac gttcaccgtg
     3841 ctcagcccgg acggcaaacg caccatcacc ttcactggga aggtgatggg cgacgagatc
     3901 gtcttcgtgc gcgaagccaa gggcgacccg ggggcgggat ccaaccaagg cggcggcttc
     3961 gggatctacg ggctcaatgg cccgaagacg gtaacagtga aacgggtgaa atagaacctc
     4021 cgtggtttgg acttggccgc gaaggcacga agacgttcgg ccaccaaggc accatgagcc
     4081 ctcggggaaa caaatcttct gagcggaaac gcatgttgta catggtcatc gaaagctttc
     4141 gtggcggcga tcccgttcct atctaccaga ggttccgcga tcgaggacgg ctcatgccga
     4201 acggcgttga gtatcgaggg agctgggtca ccgaggactt gagccgttgc tttcaggtca
     4261 tggagtgcga agatcggggt cttctcgatg agtggatggc caattggagc gacatcaccg
     4321 acttcgaagt cgtgccggtg ctgacttcgg cgcaggctcg ggaagtggtg gggccaaggc
     4381 tctagtaggt tatgcgtcag agaccctcga tctccggcat ctcaccgttc ttcatcgtcg
     4441 ccgacatccc ggcgtcgctc gcgttctatc gcgacgtgct ggggttcgaa atcacgtttc
     4501 gaggcccgac ccccgaggac gagttctttg gcatcgttcg ccgcgatggg gcgacgatca
     4561 tgttcaaggc gctcggcgtg atcgtggacg gcaaagaggt cctggtcgaa ccggtcccta
     4621 attaca
//
LOCUS       DBNGSH010000180         1816 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_183207, whole genome shotgun sequence.
ACCESSION   DBNGSH010000180 DBNGSH010000000
VERSION     DBNGSH010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_183207"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="AC16CY_04080"
     CDS             complement(<1..448)
                     /locus_tag="AC16CY_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3352988.1"
                     /translation="MDLHGFDLNLLVALDALLAEKSVTNAGRRVHLSQSAMSGVLARL
                     RHAFNDKLLVASRGSMTLTPLAESLVEPVRSILEQVHRTIIVNVPFDPATSRRIFTIA
                     ASDYAVTVLLTDALRRISKEAPGVTVVIIPLRERTPASDDLGLDLVI"
     gene            568..1383
                     /locus_tag="AC16CY_04085"
     CDS             568..1383
                     /locus_tag="AC16CY_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352989.1"
                     /translation="MSRIAFALTRSHVAWRVAASAIVLGLATVVAQAQRGGFPPAPQP
                     PRLAAPIDLTGYWVSLVTEDWRYRVATPPKGDYNSVPLNPAGRKAADAWDPGRDEAAG
                     EQCKAYGVGGVMRLPGRLHVTWQDDRTLIIETDAGTQVRTMRFGPATTGASDWQGHSQ
                     ASWDRMQGPMGAGLVFGGGGFGVGNAGGGSLKVVTRGMKPGYLRKNGVPYSGDAAMTE
                     YFDRFDLPGGDALMVVSTEVVDPEYLTQPFWTSTHFKKQRDATGWKPTPCTAR"
     gene            1380..>1816
                     /locus_tag="AC16CY_04090"
     CDS             1380..>1816
                     /locus_tag="AC16CY_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352990.1"
                     /translation="MTRHAMTIHHRSLTAVAALVALAMIDVRGQAPTAAPGPGFDLSG
                     YWSPVLHEDGMDRGAGPEIGDYGGIPINEAGRLFALSYDPSRLTLRHHQCDGYVAPYQ
                     MRALGNARVWEERDPKTHRLIAIHWFSQTFEGKRTIWMDGRPHP"
BASE COUNT          307 a          575 c          630 g          304 t
ORIGIN      
        1 ggatgacgag atcgagtccg aggtcgtcgg acgccggtgt ccgttctcgc agcgggatga
       61 tgaccacggt gacgccaggc gcctccttgc tgattcggcg aagtgcgtcg gtcaggagca
      121 cggtcacggc gtagtccgat gcggcaatgg tgaagatgcg ccgcgaggtg gcgggatcga
      181 acggcacgtt gacgatgatg gttcgatgaa cctgctcgag gatcgagcgc accggctcga
      241 ccagcgattc ggccagcggg gtaagggtca tgctgccgcg gctggcgacg agcagcttgt
      301 cgttgaacgc gtggcgcaga cgtgccagca cgccgctcat cgccgactga ctgaggtgga
      361 cgcggcggcc ggcgttggtg acgctcttct ccgccagcag cgcatcgagt gcgacgagca
      421 ggttcagatc gaaaccgtga aggtccatgg cggcctgatc accgcagcat cctacttcaa
      481 tcgctgcccg ccgcattggc aggcccgatg cccgtcattg gaacaaacga tttgcccgcc
      541 tgcagacgtg gccgcagagt agacggcgtg tcacgcatcg cgtttgcgct gacgcgctcg
      601 cacgtcgcgt ggcgggttgc ggcgagtgcg atcgtcctcg gcctcgcgac ggtggtagcc
      661 caggcccagc gcggtggatt tccacccgcg ccgcagccgc ctcggctggc tgcgccgatc
      721 gatctcaccg gatactgggt gtccctcgtc accgaggact ggcgctaccg tgtcgcgacg
      781 ccgccgaaag gcgactacaa ctcagtcccg ttgaatcccg ccgggcgcaa ggccgccgac
      841 gcgtgggatc ccgggcgtga tgaagccgcg ggggagcagt gcaaggctta tggcgtcgga
      901 ggcgtgatgc gattgcccgg ccgcctgcac gtcacgtggc aggacgaccg tacgctcatc
      961 atcgagactg acgccggaac gcaggtccgc acgatgcggt tcggtccggc gacgacgggc
     1021 gccagcgact ggcagggaca ctcgcaggca tcgtgggacc gcatgcaggg gccgatgggt
     1081 gccgggctcg tcttcggtgg cggcgggttc ggcgtcggca acgcgggcgg gggttcattg
     1141 aaggtcgtca cccggggcat gaagcccggt tacctgcgca agaacggcgt tccgtacagc
     1201 ggcgacgccg ctatgaccga gtacttcgat cgcttcgatc ttccgggcgg cgatgcgttg
     1261 atggtggtgt ccaccgaggt cgtcgatcct gagtacttga cgcagccgtt ctggaccagc
     1321 acgcatttca agaagcagcg cgacgcaacc ggctggaagc cgacgccgtg cacagcccga
     1381 tgacccgcca cgccatgacg atccaccatc gatccctgac cgcggtcgcg gcgctcgtcg
     1441 ctcttgccat gatcgatgtt cgcgggcagg cgccgacagc cgcgccgggt ccaggattcg
     1501 atctctccgg ctactggtca ccggtgcttc atgaggacgg aatggatcgc ggagccgggc
     1561 cggagattgg cgactacggc ggcatcccga tcaacgaagc cggccgtctg tttgcgctct
     1621 cgtacgatcc atcccgcctc acgcttcggc atcaccagtg tgacggctac gtggctccat
     1681 accagatgcg agcactgggg aacgcgcgtg tctgggaaga gcgcgacccg aagacgcatc
     1741 gcctcatcgc gattcactgg ttcagtcaga cgttcgaagg gaagcggacc atctggatgg
     1801 acggccggcc gcaccc
//
LOCUS       DBNGSH010000181         8401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2900240, whole genome shotgun sequence.
ACCESSION   DBNGSH010000181 DBNGSH010000000
VERSION     DBNGSH010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2900240"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="AC16CY_04095"
     CDS             complement(<1..360)
                     /locus_tag="AC16CY_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3352991.1"
                     /translation="MLKRAGLTAALAALLFGLSTTPAQATPISGAFSIFGNFLPVNGV
                     TGAATSLTSATGIDFLALFGSAPSPGTAGQFLVSSATGNFSGLVGALGSIQDMSFSGA
                     GSTSFPNPNVVGPLVAFQ"
     gene            complement(583..1740)
                     /gene="xylA"
                     /locus_tag="AC16CY_04100"
     CDS             complement(583..1740)
                     /gene="xylA"
                     /locus_tag="AC16CY_04100"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HIR3352992.1"
                     /translation="MTLQPAPSDRFTFGLWTVGNRGRDPFGEFVRPPLDPCVAVRRLG
                     EIGAWGVNFHDNDLVPIDASPSERDRIVRDFRRALEATGVTVPMATTNLFTDPVFKDG
                     AFTSHDRRVREYALQKTMRAMDLGVELGARTYVFWGGREGAETDAAKDPVEAIKRFRE
                     AIDFLCEYARDERYPLRFALEAKPNEPRGDIYFPTTAAYLAFIETLAHPEMVGVNPEV
                     AHENMIGLNVYHVVAQALEAGKLFHIDLNDQKPGRFDQDLRFGSESIKPLFFLVKLLE
                     ESGYDGPRHFDAHAYRTEDEEGVWDFARGCMRTYLILKAKVRQFAEDREIQSLLDELR
                     GPDQIPLGPYNRERARELKASAFDRQSLAARRLPYERLDQLVVDLLLGVVD"
     gene            1828..5211
                     /locus_tag="AC16CY_04105"
     CDS             1828..5211
                     /locus_tag="AC16CY_04105"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3352993.1"
                     /translation="MLRIPVGLFGLLSLVVLLLAPSDLAAQGTAQISGIVKDSSAAVL
                     PGADVTATQIETGLVRSTITNEAGAYSLPNLPVGPYRLEVMLQGFRKHERTGIVLQTN
                     TNPVIDVVMQLGEVAEAVTVTGATTLVETRSTGVGQIMDNQRILDLPLNGRNPAELLL
                     TIPAVVPMQALNATSRSMQGATGGQAYAVAGSLSFGVSYVLDGAMHNNPYDNLNLPLP
                     FPDALQEFKAETSALTAQYGMHSGAAVNAVTKSGTNVFRGNAFEFFRHHSLNATDPFA
                     ARNPDGSRKDDGLKRNQYGGTIGGPIARDQVFFFGAYQGTNTRVVPVDNLGFVPTAAM
                     LNGDFRAYASAACNNGVARTLPPPFSGNQIEPALLSKAALNISRNLPTPDNECGLITY
                     GLPDQSDDGQGVAKVDVQLNTRHSLFGRYIATSYYRPPPFDAAGGNLLVTRQGGRDQM
                     AHSVTAGENWIINPTTLNSFRFAFNRTDIHRTSTDFFSAPEMGINIYSYMPHYMLLTV
                     GAAAGANVTGFQLGGGTESESTFDTNAWQIGDDVTLVRGNHQFGVGVNAAHWSSLSLA
                     NVRSPGQLTVGNIATGLVLSDFLLGRLQQFQQAAPNTLDMTQTYVGVYAQDTWRVSPQ
                     VTLSYGLRWEPFLPPQLVNGAVYQYDQSRFDQKIKSGVFPNGPAGLYFPGDPGFPTQA
                     GMPSRWDNLAPRAGIAWDPSGNGRTVVRAAYSKAFEFVNAQFHLNTSVAPPWGSEVIL
                     ANPPGGLDNPFLGASQPNIFPVTFDQNAPFSLFGPFNSLNNDLESTTVHLWNVAFEKQ
                     LGVDWLVSAGYVGSRTLNIWETTGLNNADPTITSAVLPSGAVVNCVPGAANFQTCMTS
                     IENQRRPLIRADPVNGQYYGRLDLLVSDGRQHYNGLLLSVSRRAARGLILTANYALSK
                     CYGSPDGSGGGTPNLGSGYNDPDNPSFDDGNCTSDRRHNFSMTAGAQMPEFSQGALHA
                     IASNWRVMGSFRALTGPWLSVTPGSERALNGSGLFNQQRANQVLDDPYGDMSVDPVTR
                     GIRFLNPAAFALPPVGTLGTMKRNSIEGVGTKNVDVTLNRVFRLSGSQHVEFRAEAFN
                     VFNWFQRGQPNTNRSAGTFGLITTAGAPRILQFAVKYGF"
     gene            complement(5225..7423)
                     /locus_tag="AC16CY_04110"
     CDS             complement(5225..7423)
                     /locus_tag="AC16CY_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HIR3352994.1"
                     /translation="MNASLTDSRHAGRAFSVRLAVLASIALCLGTAARAEDGYELWLR
                     YPLVSDARLLKQYRESSAAILVAGDSATARLVREELSRGLRGLLGADIPLARDVLRDG
                     VILAGTPKSSPLIAGLRLDEDLRRAGDEGLIVRSAQVAGRRAIVIAGNTDIGVLYGAF
                     RFLRLLQTHQPIDALSIVEAPAVRHRVLNHWDNLDGTVERGYAGASLWDWHTLPDYRA
                     PRYRDYARANASIGVNGTVLTNVNANATMLTGEYLAKAAALADVFRPYGLRVYLTARF
                     SAPIEIGGLKTADPLAPEVRAWWSKKVDEIYSYIPDFGGFLVKANSEGQPGPQDYART
                     HADGANMLADALAPHNGIVMWRAFVYSENVKEDRAKQAYTEFQPLDGTFRPNVLIQVK
                     NGPIDFQPREPAHPLFGAMPRTPLMLELQITKEYLGFATHLVYLAPLFEEVLHADTHA
                     RGEGSPVRRVVDGTLHGYAVTGMAGVANTGTNRNWCGSVFACANWHAFGRLAWDPSLR
                     SAALADEWLRMTFGNVPAFIEPVKAMMLSSRQAAVDYMTPLGLHHLMARGHHYGPGPW
                     VSGGPRADWTSVYYHRADASGIGFDRTSAGSNAVGQYAPAIRESYGRADRVPEDLLLW
                     FHHLPWDHRVASGRTLWDELVERYCRGVLSVRAMQATWRAQEGRIDAERYEETRTFLA
                     IQEKEARWWRDASVLYFQTFSKRPIRDTCGPPAESLEYYLAIDRRYVPGN"
     gene            7505..7894
                     /gene="crcB"
                     /locus_tag="AC16CY_04115"
     CDS             7505..7894
                     /gene="crcB"
                     /locus_tag="AC16CY_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR3352995.1"
                     /translation="MVWLLVGAGGALGSIARHGLNRLVHQGHLESPFPLGIFLVNVSG
                     SAAIGVLSGVIASGRMELSLEARTFLIVGLLGGFTTFSSFSLDSLALIRGGHLGQAIW
                     NVVGQVGLSLIAVWLGFAVGGKVGGMV"
     gene            complement(7917..8279)
                     /locus_tag="AC16CY_04120"
     CDS             complement(7917..8279)
                     /locus_tag="AC16CY_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 family protein"
                     /protein_id="HIR3352996.1"
                     /translation="MNWTHLIYGLHAFSLLTGIFGTATVVGMFLTGWPSLIAVILNYV
                     KRSDVRGTWLESHFRWQIRTFWFGLLWILLCILFVFMTLGLGMLVVWIPVGLVALWFI
                     YRVARGWIALNDRRPMTV"
BASE COUNT         1533 a         2722 c         2720 g         1426 t
ORIGIN      
        1 ttgaaacgcg acaagcggac ccaccacatt cgggttcggg aagctcgtgc tgccggcccc
       61 ggaaaacgac atgtcttgaa tcgatcccag cgcaccgacc agtccggaaa agttgcccgt
      121 cgcagaactc acgaggaact gaccggcagt gcccggagac ggcgctgatc cgaagagcgc
      181 cagaaagtcg atgcccgtcg ccgaagtgag agaggtcgcg gcaccagtga cgccattgac
      241 gggcaggaag ttgccgaata tcgaaaaggc tccgctaatc ggcgtggcct gcgccggggt
      301 cgtactcagg ccgaacaaca gagccgctaa cgcggccgta agtcccgcac gcttcaacat
      361 ttgtcctccg acgtccctgt tggtgcacca ataccggcgc gttccgcagg tgaaatagca
      421 agctccctgc cgaggctctg ccgtaatggc aagctagaca caacgccatc agaatcagtg
      481 gtttgagaga tcggcagcga aatctgtgac cgggaaaatc ctcacctagt cgcaccgcgg
      541 gctgacatta ggggacctgc cgggccacag tcggccctcg cctcagtcca caacgccgag
      601 caacagatcg accacgagct ggtcgaggcg ttcgtagggg aggcggcgcg cggcaaggct
      661 ctgacgatcg aaggcgctcg ccttgagctc gcgggcgcgt tcgcggttat agggtccgag
      721 agggatctga tccgggccgc gcagttcgtc aagaagggac tggatctccc ggtcctccgc
      781 gaactgccgc acctttgcct tgaggatcag gtaggtccgc atgcacccgc gcgcaaagtc
      841 ccacacgcct tcttcgtcct ccgtgcggta ggcgtgcgcg tcgaagtggc gcggaccgtc
      901 gtagcccgat tcctccagca gcttcacgag aaagaacaac ggcttgatcg attcggatcc
      961 gaagcgcagg tcctgatcga agcgcccggg tttctggtcg ttcaagtcga tgtggaacag
     1021 cttcccagcc tccagcgcct gtgcgacgac gtggtagacg ttcaacccga tcatgttctc
     1081 gtgcgcgact tccgggttga cgccaaccat ctccggatgc gcgagtgtct cgatgaacgc
     1141 caggtaggcc gcggtggtcg gaaaatagat gtcgcccctg ggctcgttgg gcttggcttc
     1201 aagggcgaac cgcagcgggt atcgctcgtc gcgcgcgtac tcgcagagga agtcgatcgc
     1261 ttcgcggaac cgcttgatgg cttcgaccgg gtcctttgcc gcatcggtct ccgccccttc
     1321 ccttcctccc cagaacacgt aggtgcgcgc gccaagctcc acgccgagat ccatcgccct
     1381 catcgtcttc tgcagcgcgt actcgcgaac gcgcctgtcg tgcgacgtga acgcgccgtc
     1441 cttgaacacg ggatcggtga acagattcgt ggtggccatc ggcacggtga cgccggtcgc
     1501 ctccagcgcc cgccggaagt cgcgcacgat ccggtcgcgc tcggacggcg acgcgtcaat
     1561 cggcacgagg tcgttgtcgt ggaagttgac gccccaggcg ccgatctccc ccagacgacg
     1621 gacggcgacg cacggatcga gcggcggccg gacgaattcg ccgaacggat cgcgtccgcg
     1681 gttgccgacg gtccagaggc cgaaggtgaa gcggtccgag ggagcgggtt gaagggtcat
     1741 ggtgtccgtc aatgctatac tccgccgcga attcgccaca atccagcgaa cgaagccgtc
     1801 gtcctttcaa gcggaggagg ttcgatgatg ctgcgtattc ctgtcgggct gttcggcctt
     1861 ctgtccctcg ttgtgctcct gctcgctccc agcgatctgg cggctcaggg cactgctcaa
     1921 atcagcggca tcgtgaaaga ctcgagcgcg gcagtcctgc ccggcgccga cgtgacagcg
     1981 acccagatcg agaccggtct cgtgcggagc acgatcacga acgaggctgg cgcgtacagc
     2041 ctgccgaacc tcccggtggg tccgtacagg ctcgaggtga tgcttcaggg ctttcgcaag
     2101 cacgagcgca ccggcatcgt cctgcagacc aacacgaacc cggtgatcga cgtcgtgatg
     2161 cagctcggcg aggtcgccga agcggtcacc gtgaccgggg cgacgacgct cgtcgagacc
     2221 cgcagcaccg gtgtgggtca gatcatggac aaccagcgga tcctggatct tccgctcaac
     2281 ggccgaaatc ccgccgaact cctcttgacg attccagccg tggtgcccat gcaggccttg
     2341 aacgccacga gccggagcat gcagggggca acgggcggtc aggcctacgc ggtcgccggc
     2401 agcctgtcgt tcggtgtgtc gtacgtgctc gacggggcga tgcacaacaa tccgtacgac
     2461 aacctgaatc tgccgctgcc gttccctgat gcgcttcagg agttcaaggc ggagacgagc
     2521 gcgttaacgg cgcaatacgg catgcactcg ggagccgccg tcaacgccgt gaccaagtca
     2581 ggcacaaatg tctttcgagg caacgcgttc gagttcttcc gccatcacag cctcaacgcc
     2641 acggatccat ttgccgccag gaatcccgac ggctcgcgaa aagacgacgg cctgaagcgt
     2701 aaccagtacg gcgggacaat cggcgggccg atcgcgaggg accaggtgtt cttcttcggc
     2761 gcgtaccagg gcacgaacac gcgggtcgtg ccggtcgaca atctcgggtt cgttccgacc
     2821 gccgcgatgc tgaacggcga cttccgggcc tatgcgtcgg ccgcgtgcaa caacggcgtg
     2881 gcgcgaaccc tgccgccgcc cttctccggc aatcaaatcg aacccgcgtt gctcagcaag
     2941 gctgcgttga acatctcgcg gaatctcccc acgcccgata acgaatgcgg tctgattacg
     3001 tacgggctgc ccgaccagag cgacgacggg cagggtgtcg cgaaagtcga cgtgcagctg
     3061 aacacgcggc actcgctgtt cgggcggtac atcgcgacga gctactatcg accgccgccc
     3121 ttcgacgcgg ccggcggaaa tctgctcgtg acccgccagg gaggccgcga ccagatggct
     3181 cacagcgtca ctgccggcga gaactggatc atcaatccca cgacgctgaa ctcgttccgg
     3241 ttcgcgttca accgaaccga catccatcgc acgagcaccg acttcttttc ggcgcctgag
     3301 atgggcatca acatctacag ctacatgccg cactacatgc tgctgactgt gggcgcggcg
     3361 gccggcgcga acgtcaccgg attccagctc ggcggcggca ctgaaagcga gtcgaccttc
     3421 gacaccaacg cctggcagat cggcgacgac gtgacgctgg tccgcggcaa ccaccagttc
     3481 ggcgtcggcg tcaacgccgc ccactggtcg tcgctctcgc tggccaacgt ccgctccccc
     3541 ggtcagctca cggtcggcaa catcgcgaca ggcctcgtcc tgtcggactt ccttcttgga
     3601 cggctgcagc agttccagca ggcggcgccg aacacgctcg acatgacgca gacctacgtc
     3661 ggcgtgtacg cgcaggacac gtggagggtg agtccgcagg tgacgctcag ctacgggctc
     3721 cgctgggagc cgttcctgcc gccgcaactc gtcaacggag ccgtctatca gtacgaccag
     3781 agccggttcg accagaagat caagagcggc gtcttcccca acggccctgc gggcctgtat
     3841 ttcccgggtg atcctggctt cccgacacaa gcggggatgc cgagccggtg ggacaacctg
     3901 gcgccacgcg cggggatcgc ctgggatccg tcagggaacg ggcggaccgt cgtgagggcc
     3961 gcctacagca aggcgttcga gttcgtcaac gcgcagttcc acctcaatac gtccgtggcg
     4021 ccgccgtggg gctctgaagt catcctggcc aacccgcctg gcggcctcga caatccattc
     4081 ctcggtgcgt cacagccgaa catcttcccc gtcacattcg atcagaatgc gcccttctcg
     4141 ctcttcgggc cgttcaacag cctgaataac gatctcgaat cgacgacggt gcacctgtgg
     4201 aacgtggcgt tcgaaaagca gctcggcgtc gactggctcg tgtcggccgg ctacgtcggc
     4261 agccgcacgc tgaacatctg ggagacgacc gggctgaaca acgccgaccc cacgatcacc
     4321 tcggccgttc tcccgagcgg cgcggtcgtg aactgcgtcc ccggcgccgc gaatttccag
     4381 acctgcatga cgtcgatcga gaatcagcgc cgcccgctga tccgggccga tcccgtcaac
     4441 gggcagtact acggacggtt ggacctgctc gtcagcgacg gcaggcaaca ctacaacggc
     4501 ttgctgctga gcgtgtcgcg ccgcgccgcg aggggcctga tcctcacggc caactacgcg
     4561 ctctccaagt gctacggatc gcccgatggc agcggcggtg gaacgccgaa cctcggctcg
     4621 ggctacaacg accctgacaa tccgagcttc gatgacggga actgcacatc ggatcgccgc
     4681 cataacttct cgatgaccgc cggggcccag atgcccgagt tcagtcaggg agcgctgcac
     4741 gcgatcgcgt cgaactggag ggtgatggga agcttccggg ccctgacggg gccgtggctg
     4801 tcggtgacgc cgggttccga gcgcgcgttg aacggatcgg gcctcttcaa ccagcagcgc
     4861 gccaatcagg tgctcgacga tccgtacggc gacatgagtg tcgaccctgt cacgaggggc
     4921 atccgattcc tgaacccggc cgctttcgct ctgccaccgg tcggtacgct gggtacgatg
     4981 aagaggaaca gcatcgaagg cgttggtacc aagaacgtgg acgtcacgtt gaacagggtc
     5041 ttccgtctct cgggcagcca gcacgtcgaa ttccgggcgg aggcgttcaa cgtgttcaac
     5101 tggttccagc gaggacagcc gaacacgaac cggagcgccg ggacgttcgg gctgatcacg
     5161 acggcgggcg cgccgcgaat cctgcagttc gccgtgaagt acggattctg aggggcgaac
     5221 gaggtcagtt cccgggaacg tagcgccggt cgatcgccag gtagtactcc agcgattcgg
     5281 ccggcggccc gcaggtgtcc ctgatcggcc gcttcgagaa cgtctggaag tacagcacgc
     5341 tcgcgtcgcg ccaccaccgc gcctccttct cctggatggc gaggaatgtg cgcgtctctt
     5401 cgtaccgctc cgcgtcgatg cggccctcct gggcgcgcca ggtggcttgc atcgcgcgga
     5461 cggacagcac gccgcggcag tatcgctcga cgagctcgtc ccagagcgta cgcccggatg
     5521 ccacacggtg atcccaggga agatgatgaa accagagcag gagatcctcc ggcacgcggt
     5581 ccgcgcgccc gtagctctcg cggatggccg gcgcgtactg ccctaccgcg ttgctgccgg
     5641 ccgacgttcg atcgaaaccg atgccagagg catcggcgcg atggtagtag accgacgtcc
     5701 aatcggccct gggcccgccg ctgacccacg gtcccggacc gtagtggtgg cctcgagcca
     5761 tgaggtgatg caggccgagc ggcgtcatgt agtcgaccgc cgcctgccga gaggacagca
     5821 tcatcgcttt caccggttcg atgaaggccg ggacgttgcc gaacgtcatg cgcagccact
     5881 cgtcggcaag cgcggcggaa cgaagcgacg ggtcccaggc cagccggccg aacgcgtgcc
     5941 agttggcgca cgcgaacacg gagccgcacc agttgcggtt cgtgccggtg ttggcgacgc
     6001 cggccattcc ggtcactgcg tacccgtgaa gtgttccgtc gacgacgcgc ctgacgggcg
     6061 acccctcgcc tcgcgcgtgc gtgtcggcat gaagcacttc ttcgaagagc ggcgcgagat
     6121 agaccagatg cgtggcgaac ccgagatact ccttcgtaat ctgcagctcg agcatcagag
     6181 gcgtccgcgg catcgcgccg aagagcggat gcgcgggctc gcgaggctgg aagtcgatcg
     6241 gaccgttctt gacctggatg aggacgttcg gccggaaggt tccgtcgagg ggctggaatt
     6301 cggtgtacgc ctgcttcgct cgatcctcct tcacgttctc cgagtagacg aaggcccgcc
     6361 acatgacgat gccgttatgc ggagccaacg cgtcggccag catgttcgcg ccgtcggcgt
     6421 gcgtgcgggc gtagtcctgc ggccccggct ggccctccga atttgccttc acgagaaacc
     6481 cgccgaagtc gggaatgtag gaatagatct cgtcaacctt cttcgaccac caggccctca
     6541 cctccggcgc cagcggatcc gcggtcttca ggccgccgat ctcgatgggc gcgctgaaac
     6601 gcgccgtcag gtacacgcgg aggccgtagg gcctgaagac gtcggcaagc gcggcggcct
     6661 tggccagata ctcgcctgtg agcatcgtcg cgttggcgtt cacattggtc agcaccgtgc
     6721 cgttgacgcc gatcgaggcg ttggcgcggg catagtcgcg gtagcgcggc gcgcggtagt
     6781 cgggcagcgt gtgccagtcc cagagtgacg caccggcata gccgcgctcg acggtgccat
     6841 ccaggttgtc ccagtggttc agcacacggt ggcgaacggc tggagcctcg acgatcgaga
     6901 gcgcatcgat gggctggtgc gtctgcagca gccgcaggaa tcgaaacgcg ccgtacagga
     6961 cgccgatgtc ggtattaccg gcgatgacga tcgcccgtcg tccggccacc tgcgccgagc
     7021 ggacgatcag accttcgtcg cccgcgcgcc tgagatcctc gtcgagccgc aatccggcga
     7081 taagcggcga gctcttcggc gtccccgcga ggatcacgcc atctcgcaag acgtcgcgcg
     7141 cgagcgggat gtcggcgccg agcagaccgc gaagaccgcg agagagctcc tcgcgtacga
     7201 gtcgcgcggt cgccgagtct ccagcaacca ggatcgccgc ggacgattcc cggtactgtt
     7261 tcaagaggcg ggcgtcggac acgagcgggt agcgcagcca cagctcgtat ccgtcctctg
     7321 cccgcgccgc cgttcccagg cagagtgcga tcgacgcgag gaccgcaaga cgaacactga
     7381 atgctcgccc ggcgtgtctg gaatctgtca gcgatgcgtt cacgtgaggc gctggtcgtc
     7441 ctgtgcgcat cctacatcag ggtgaagcag ttgacggaca atgactcctc aggagggcca
     7501 cggcatggtg tggttattgg tcggcgcggg tggtgcgctg gggagcatcg cgcgccacgg
     7561 tctcaatcgg ctcgttcatc aaggacactt ggaaagcccg tttccgctcg gtatctttct
     7621 ggtcaacgtc tccgggtcgg ccgccattgg cgtgctcagc ggcgtcatcg cctccgggcg
     7681 gatggagctg agtctcgagg cgcgcacgtt cttgatcgtc ggcctgctcg gcggcttcac
     7741 gacgttttcc tctttcagtc tcgactcgct cgcgctgatt cgcggcgggc atctgggcca
     7801 ggcgatctgg aacgtggttg gccaggtcgg tctgagtctg atcgcggtct ggcttggatt
     7861 cgccgtcggc ggaaaggtcg gagggatggt ctgatgaagc gcgctatcgc gcgggctcag
     7921 accgtcattg gccggcggtc gttgagcgcg atccagccgc gggcgaccct gtagatgaac
     7981 cagagcgcca cgaggccgac cggtatccac accacgagca tgcccagccc cagggtcatg
     8041 aagacgaaca ggatgcagag caggatccac agcagcccga accagaacgt gcggatctgc
     8101 cagcggaagt gcgactcgag ccaagtgcct cgcacgtcgc tccgcttcac gtagttcagg
     8161 atgacggcga tgagcgacgg ccagccggtc aggaacatgc cgacgaccgt ggcggtaccg
     8221 aaaatgccgg tgaggaggct gaacgcatgc aacccgtaga tcagatgcgt ccaattcacg
     8281 agagaacgct cgtcgggcga gttcattttc gcgtcaccgg aatatcgaga ttgtccaacc
     8341 cgttcttgta gtcagggtcc atggcggcga tgtcagtacg ataggtgacg gtgtcggcag
     8401 t
//
LOCUS       DBNGSH010000182         9379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3409626, whole genome shotgun sequence.
ACCESSION   DBNGSH010000182 DBNGSH010000000
VERSION     DBNGSH010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3409626"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1797)
                     /gene="tagF"
                     /locus_tag="AC16CY_04125"
     CDS             complement(313..1797)
                     /gene="tagF"
                     /locus_tag="AC16CY_04125"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HIR3352997.1"
                     /translation="MAVPLDVGFYGKLPSHGDFLRRRVSDAFVGVWDGWLQECLAASR
                     SALADRWLDVYLTSPVWRFACAAGACGSAPVVGVMAPSVDRVGRYFPLTLVAELPPDT
                     PTLLASVLGANGFFDGAERLVIETLERDTIDLETFDEQLVGLGQTLERSGAAAGVVLE
                     GGAALLLNDGAEGWQLPIGSAGDLGGTFTQLVSQRLSDLYGPMVLWWTNGSSIVPPSC
                     LVVKGLPHPDSFAGLLDGSWRERGWRSVAAHVETAQLESEPLVEDLTPPRFRSAAASD
                     VGRVRQINQDSFLERTDVGIWAVADGLGGHSDGEVASRMVCDALAEVVPDSSFEDLVD
                     EVRRQVGSVNELLVRAATRPVNAVQSGSTIVTLLARGSRCVVLWAGDSRAYRSRNGQL
                     EQLTRDHNLVSEDGDDSVDLHAITRAVGGEETLTLDQYRDRVFPGDRFLLCSDGLTRT
                     VPHAQLQGWMAEADIRQVVDNLISAALKAGGPDNVTAVVVEAFA"
     gene            complement(1812..5285)
                     /gene="tssM"
                     /locus_tag="AC16CY_04130"
     CDS             complement(1812..5285)
                     /gene="tssM"
                     /locus_tag="AC16CY_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HIR3352998.1"
                     /translation="MAIFSFLKSRILLVASGFVLLALFIWYVGPLFGIGDYKPLDPVL
                     NRLIAIALVVVVWAGTALARRLRASRASDKLVAAVVQQSRAEERPSAEALQLRERFEE
                     AVAVLKQKRRRGHSLYDLPWYVIIGAPGSGKTTALVNSGLNFPLEQRSGKGALRGVGG
                     TRNCDWWFTDEAVLLDTAGRYTTQDSDASADSAGWSEFLALLKKYRKRRPVNGVILTI
                     SAHDLMVQGHAGRDAQAAATRRRLNELNKELNIQLPVYLMVTKCDLVAGFTEYFDDLA
                     QEGRAQVWGVTFPYELTTSGKAAGSFASEFDALTARLNERVFPRLEEERDPRRRVKAF
                     AFPQQFAALRELLDGFIADVFASTRFDQQILLRGVYFTSGTQEGTPIDRLLGALGRRF
                     AMAPDAVTPSGRGRAYFIERLLKEVLFAESGLAGVNRRVEVQKAAAQLGAYVAMALVA
                     VLGFIAFSISFSRNRTYIGEVDADVKAFQQAPKEAGNTLETILPRLNAVQKVSDSANR
                     YRDVEPWAMRWGLYQGSSLGNAARDAYSRELAGVLLPQVTSRIEQRLASYSSEPEKLY
                     EYFKAYLMLGDPKRLDKQQLGYIADLEWKAAYAADPETGAAVSRHFRNLLEYPDRLRA
                     MTLDQSLVARARSTIQQASIPQLVYRQIRLAYSDDSPLRLDVAAGVGAEQVFRLKSGA
                     SLSKPVPRIYTAQGFREVTSQSADELVRAYASDYWVWGEGRPSLTASSKLKDDVLDLY
                     EKDYIAAWDAILSDIELASFPNLAATSNGLAILAGSPSPFRGILKIVDDNTYLVPPAD
                     PSKSASTAATVKDKLEGIFQDVKRGAGLSTRVPGVQVTEYFARIHQMVGGEVGKAPVD
                     GVISKIGEIQRQLAAVGTRYGEVPPLEALTRAGQGGTMKSLQQDATTMPPQVGAIVTQ
                     ITGRAAAAATVEAGNEFMNRYSQDVLAPCRQFISGRYPFNASSQTDVALGDFGRLFGQ
                     DGIFDRFFKANLQNLVDTSRRPWVLREDQSGGSVGISGSVLRQFEAAQRIRDKFFGNG
                     GQMPQVRFTVTPITMDGTLSRFVLEIDGQTFTYQFGPERSQQAQWPGQMPVAAATFED
                     RASGRPNIAFQGQWAWFRLLDVAQTRSESDLRHEVTFEKSGFKATVRIEAASVLNPFG
                     GRDLQQFRCEV"
     gene            complement(5287..6633)
                     /gene="icmH"
                     /locus_tag="AC16CY_04135"
     CDS             complement(5287..6633)
                     /gene="icmH"
                     /locus_tag="AC16CY_04135"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HIR3352999.1"
                     /translation="MSDNDDPFKPSDATVLRPRPGGGRRPSGSPATRGPGSPQGYADS
                     TSFGPRPGASTEPISSSLQESLGVGLNPLVRVASPLLMLAGQLRGSLSAPDISGLRRL
                     ALDEIRRFEDRARASGIQSEVVLAARYALCAGVDEAVLSTPWGAQSEWAQQTLLVALH
                     REAQGGEKFFEMLDRISGDPARHIDLMELQYLCVALGFAGKYQVVDRGHAQLAEVQHD
                     LYRRIRNHRGVPQPELSLRWRGLQDRRNPVIRYVPWWVVGAAALAIVGLTFVVYYARL
                     GSAAAPVHTSLAGIGLEDFTTPRPAVPSPGPTLKELLAPDEARGTLSVQEDGGLTRIT
                     LRAPDLFASASATLNPAQAGTVASVAAALNKVPGRVLVEGHTDDQPLRSFKYRDNFEL
                     SRERAVTVGRMLQSALDNPARVEWNGVGSSKPLHPEPTPENRARNRRVEIVHVRGT"
     gene            complement(6648..7988)
                     /gene="tssK"
                     /locus_tag="AC16CY_04140"
     CDS             complement(6648..7988)
                     /gene="tssK"
                     /locus_tag="AC16CY_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HIR3353000.1"
                     /translation="MSAYNKVVWSEGLFLRPQHFQQQDRYFERYVETRCQALVPHSWG
                     FSDLEIEPDLLSIGKFGLRRASGVFPDGTPMRMPEDDPLPAPIEIGPQVRDQTVYLAV
                     PVRRSGAAEVERQSQSGADGLARHAIRELDARDSTAGGHDAVVLEVGALRSRFLFASD
                     VTDAYACVPLAHVTECRADRRVVLDEGFIPTVLDTRASRRLTTFTTELLGLLHQRGEA
                     LGGRVSASSRGAAAEIADFLMLQAINRYEPLLAHLVDSGVLHPEDLFRICVSAAGELS
                     TFTTAAKRPPKVEGYRHDRLRESFEPVMAALRASLSAVLEQAAIPIPIESKKFGISVA
                     TVADRGLYSTAVFVLAARADVAAEDLRRRFPTQLKIGPVEKIRDLVNLQLPGVPVHPM
                     PVAPRQIPYHAGFVYFELDQANELWGQLKSSGGVALHVAGEFPGLALEFWAIRS"
     gene            complement(8025..8489)
                     /gene="tssJ"
                     /locus_tag="AC16CY_04145"
     CDS             complement(8025..8489)
                     /gene="tssJ"
                     /locus_tag="AC16CY_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HIR3353001.1"
                     /translation="MRIHHRAVVRAAALVAMLVAAACASAPPKPTTTNLTFAATADSN
                     PDANGRASPVVVRLYQLKADTAFSAAEFFALYDDEKKALGPELIGRDEFVLAPGQNHT
                     VQVAMSKDARFVGMIAAYRDIRNAQWRVVVPAPIRNNATIRVERARIQYSVK"
     gene            complement(8511..>9379)
                     /gene="tagH"
                     /locus_tag="AC16CY_04150"
     CDS             complement(8511..>9379)
                     /gene="tagH"
                     /locus_tag="AC16CY_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HIR3353002.1"
                     /translation="DLAAGSLLSEHYQAPEPVRSEPPPSQGAGLIPDDYDPLQSGPVD
                     VASAARRPPAQVRSPADAEPPRQARPAHGAAAGRAAQGQAKPSSAASSDAVLQALLEG
                     AGLDASAVTPEFARSFGQILRVVVAGVMDVLQARQRIKDEFRLRVTTFKPTQNNPLKF
                     SANVEDALHNLLVKRNPAYLGPVAAFEDAFDDIRSHQMAMLAGVRVAFEAMLAEFHPD
                     RLQEEFDRQGKGSLLAGPGKLRHWDQYRAKFSDMVSDADASFRELFGDEFAKAYEEQL
                     ERLKGRDRARTP"
BASE COUNT         1548 a         3211 c         3122 g         1498 t
ORIGIN      
        1 ccagcgtgtc ggtgtcctcc aggttcttgc cgcacggttg atccgccgaa atgggctcga
       61 gcaggccgtg cccgtcccaa cgggcgttca tacgcggccc cgggccgcag cacgcgcggc
      121 gatcacactc tgtaaaacga ccggcgtcat cacggcgaac tccaccccgt ttcgaggagt
      181 cggattctat cggcttctct tgaaaggcga aagcccaaag tgccgcctgt ggaccaccaa
      241 ttcctgaggt gacatactgg gaagtcctgt aaacgtgggc agttccatca gggcgaaggc
      301 gccttgactg cgtcaggcga aggcttccac gaccacggcc gtgacgttgt ccggcccgcc
      361 ggccttcaga gccgcagaaa tcaggttgtc aacgacctgg cgaatgtcgg cctctgccat
      421 ccagccctgc aactgtgcgt gcggcaccgt gcgtgtcagc ccgtcggaac acagcagaaa
      481 gcgatcaccc ggaaagaccc ggtcgcgata ctgatcgagc gtcagcgttt cctcgcctcc
      541 caccgcgcgg gtgatcgcgt gcaggtcgac cgagtcgtcc ccgtcttcgg acacgagatt
      601 gtggtctcgc gtcaactgct cgagctggcc attccgcgat cggtaggctc ggctgtcgcc
      661 agcccacaag acgacacagc ggcttccccg cgcgagcagc gtgacgatgg tgctgccgct
      721 ctggaccgca ttgacggggc gtgtggcggc ccggaccagg agctcgttca cggatccgac
      781 ctggcggcga acctcgtcaa cgagatcctc gaacgaggag tcgggcacca cttcggccag
      841 tgcgtcgcac accatccggc tcgcgacctc tccgtcgctg tgtccgccga ggccgtcggc
      901 caccgcccag atgccgacgt cggttcgctc gagaaacgag tcctggttga tctggcggac
      961 ccggcccacg tcgctggccg cggccgacct gaagcgcggc ggcgtcagat cctcgacgag
     1021 aggctcgctt tcgagctgcg cggtctcgac gtgcgccgcc accgagcgcc acccgcgctc
     1081 gcgccacgac ccgtcgagca acccggcaaa cgagtcggga tgaggcagcc ccttcacgac
     1141 gagacaactc ggcggcacga tcgacgagcc gttcgtccac cagagcacca tcgggccgta
     1201 cagatcagac agccgttgtg acacgagctg cgtaaaggtt cctccgaggt cgccggccga
     1261 gccgatcgga agctgccacc cctcggcacc gtcgttgagc agcaaggccg cgccgccttc
     1321 gaggacgaca ccggccgcgg caccggagcg ttcgagcgtt tgccccaggc cgacaagctg
     1381 ctcgtcgaag gtttcgaggt cgatcgtgtc tcgttcgagc gtctcgatca ccagccgctc
     1441 ggcgccgtcg aagaagccgt tcgcacccag gacggaggca agcagggtcg gggtgtccgg
     1501 cggcagttcc gccaccaggg tcagcggaaa gtaccgtccg acgcggtcga cgctcggcgc
     1561 catcacgccg acgacgggag cgctgccgca cgcacccgcg gcgcacgcga atcgccagac
     1621 cgggctggtc aggtagacgt cgagccagcg atcggcgagc gccgatcggc tcgcggccag
     1681 gcactcctgc agccagccgt cccagacgcc gacgaaggcg tcagagacgc gccgcctgag
     1741 aaagtcgccg tggcttggca gcttgccgta gaacccaacg tcgaggggaa cagccatggt
     1801 gcacccggca actacacctc gcagcggaac tgctgcagat cgcgcccgcc gaacggattg
     1861 aggacgctcg cggcctcgat ccgcacggtc gccttgaagc cgctcttctc gaaggtcacc
     1921 tcatggcgca gatccgactc gctcctggtc tgtgccacgt cgaggagccg gaaccaggcc
     1981 cactgtccct ggaacgcgat gttgggccga ccgctggcgc gatcttcaaa ggtcgccgcc
     2041 gcgacgggca tctggccggg ccactgcgcc tgctgagagc gctccggccc gaattgatag
     2101 gtgaaggttt ggccgtcgat ctccaggacg aagcggctga gcgtgccgtc catggtgatg
     2161 ggcgtcacgg tgaaccgcac ctgcggcatc tgcccaccgt tgccgaagaa cttgtcccgg
     2221 atccgctgcg ctgcctcgaa ctgccgcagc acggagcccg agatcccgac cgacccgccc
     2281 gattgatcct cgcgcaggac ccacggacgc cgggacgtat ccaccaggtt ctgcaggttc
     2341 gccttgaaga agcggtcgaa gatgccgtcc tgcccgaaga gccggccgaa gtccccgagc
     2401 gcgacgtcgg tctggctgct cgcgttgaac ggatacctgc cggagatgaa ctgccggcac
     2461 ggcgccagga cgtcctggct gtaccggttc atgaattcgt tgcccgcttc gactgtcgcg
     2521 gccgccgcgg cgcggccggt aatctgcgtg acgatcgcgc cgacctgcgg tggcatcgtc
     2581 gtcgcgtcct gctggagcga tttcatggtg ccgccctggc cggcgcgtgt cagcgcctcg
     2641 agcggcggta cttctccgta cctggtgcca accgcggcga gttgcctctg gatctcgccg
     2701 atcttcgaaa tgactccatc gacgggcgcc ttaccaacct ccccacccac catctgatga
     2761 atgcgggcga agtactcggt gacctggacg ccgggaacgc gagtgctgag gcccgcgccc
     2821 ctcttgacat cctgaaaaat tccctcgagt ttgtccttca ccgtcgcggc tgtgctcgcc
     2881 gacttcgacg ggtcggcagg cggcacgaga tacgtgttgt cgtcgacgat cttcaggatc
     2941 ccgcgaaacg gcgacggcga cccggcgagg atcgccagac cgttcgacgt ggcggccagg
     3001 ttcgggaacg aagcgagctc gatgtcgctc aggatcgcgt cccacgccgc gatgtagtcc
     3061 ttctcgtaca ggtcgaggac gtcgtccttc agcttgctcg acgcggtcag cgacggccgc
     3121 ccttcacccc acacccagta gtcgcttgcg tacgccctga cgagttcgtc cgcgctctgc
     3181 gacgtcactt ccctgaaccc ctgtgcggtg tagatgcgcg gcacgggttt cgagaggctg
     3241 gcgccgctct tgaggcggaa cacctgctcg gcgccgacac cggcggcgac gtcgagcctc
     3301 agggggctgt catcggaata cgcgagcctg atctgccggt agacgagctg cggaatcgac
     3361 gcctgctgaa tcgtgctgcg ggcccgggca acgagtgatt gatccagcgt catcgcgcgc
     3421 agtcgatccg ggtactcgag gagattcctg aagtgcctcg agacggccgc tccggtctcc
     3481 gggtcggccg catacgccgc cttccactcc aggtccgcga tgtacccgag ctgctgcttg
     3541 tccaggcgct tcggatcgcc aagcatcagg tacgccttga agtactcgta gagcttctcc
     3601 ggctccgacg agtagctggc cagccgctgt tcgattcggc tcgtcacctg cggcagcagg
     3661 accccggcca gctcacgcga ataggcatcc ctggcggcgt tgccgagcga gcttccctgg
     3721 tagagccccc atcgcatcgc ccacggctcg acgtcgcgat accggttggc ggaatccgac
     3781 accttctgca cggcgttcag gcgcggcagg atcgtctcga gcgtgttgcc ggcctctttc
     3841 ggagcctgct ggaacgcctt gacgtcggcg tccacctcgc cgatataggt gcggttcctg
     3901 ctgaagctga tcgagaacgc gatgaagccg agcaccgcga cgagcgccat cgcgacgtag
     3961 gcgccgagct gggccgccgc cttctgcact tccacacggc ggttgacgcc ggcgaggccg
     4021 gactcggcga acaggacttc cttgagcaga cgctcgatga agtacgccct gccccggcct
     4081 gagggcgtca ccgcgtcggg cgccatcgcg aagcggcggc cgagcgcgcc gagcaggcgg
     4141 tcgatcggcg tcccttcctg ggtgccgctc gtaaagtaga cgccgcgcag gagaatctgc
     4201 tggtcgaagc gcgtggacgc aaagacgtcg gcgatgaacc cgtcgagcag ctcgcgcagc
     4261 gcggcgaact gctgcgggaa ggcaaaagcc ttcacccggc ggcgcggatc tcgctcctcc
     4321 tcgagacgcg ggaagacacg ctcgttcaac cgcgcggtca gcgcgtcgaa ctcggacgcg
     4381 aacgagcctg ccgcctttcc gctggtggtc agctcgtagg ggaacgtcac tccccacacc
     4441 tgcgcgcgtc cttcctgggc caggtcgtcg aagtactcgg tgaaccccgc gacgaggtcg
     4501 cacttggtga ccatcaggta caccggcagc tgaatgttca gttccttgtt cagctcgttc
     4561 agccggcgtc gcgtggccgc cgcctgcgcg tcgcggccgg cgtgcccctg caccatgagg
     4621 tcgtgagcgc tgatggtgag gatgacgccg ttcacgggtc tccgcttccg gtacttcttc
     4681 agcagggcga ggaactccga ccagccggcg ctgtcggccg aggcatcgga gtcctgcgtc
     4741 gtgtagcgcc ccgcggtgtc gagcaggacc gcttcgtcgg tgaaccacca gtcgcagttg
     4801 cgcgtgccgc cgacgccgcg cagcgcgccc ttgcccgacc gctgctcgag cgggaaattg
     4861 agcccggagt tgacgagcgc tgtcgtcttg cccgaccccg gtgcgccgat gatcacgtac
     4921 cacggcaggt cgtagaggct gtgtcctcga cgccgcttct gcttgagaac ggcgacggct
     4981 tcctcgaacc gctcgcgcaa ctggagggcc tcggcgctcg gtcgttcttc ggcgcgcgac
     5041 tgctgcacga cggccgcgac gagtttgtcg ctcgccctgc tcgcgcgcag ccgcctggcc
     5101 agcgcagtac ccgcccacac gacgacgacg agggcgatcg caatcagccg gttgaggacc
     5161 ggatcgagcg gcttgtagtc cccaatcccg aagaggggac ccacatacca gatgaacagc
     5221 gccagcaaca cgaagcccga cgcgacgagt aggatgcgac tcttgaggaa ggagaagatc
     5281 gccatctcaa gtcccccgca cgtggacgat ctccacgcgt cgattgcgcg cccggttctc
     5341 cggcgtgggc tccggatgga gcggcttcga ggagccgacg ccgttccact cgacacgcgc
     5401 cgggttgtcg agagcgctct gcagcatgcg gccgacggtg actgcccgct cgcgcgagag
     5461 ctcgaagttg tcgcgatact tgaacgacct cagcggctga tcgtcggtat ggccttcgac
     5521 cagcacgcgg cccggcacct tgttgagcgc cgccgcaacg ctggccaccg tccctgcctg
     5581 ggccgggttg agcgtcgcgc ttgcggatgc gaacaggtcg ggcgcgcgca gcgtgatgcg
     5641 cgtgagcccg ccatcctctt gtacgctgag cgtccccctg gcttcgtcgg gagcgagcag
     5701 ctctttgagc gtcggcccgg gactaggcac ggccgggcgg ggtgtggtga aatcttccag
     5761 gccgatgccg gccagcgagg tgtgcacggg cgcggcggcg ctcccgaggc gggcgtagta
     5821 gacgacgaat gtcagaccca cgatcgccag cgccgcggca ccgacgaccc accacgggac
     5881 gtacctgatg acggggttgc ggcgatcctg cagtccgcgc cagcgcagcg acagctcggg
     5941 ctgcggcacg cctcgatggt tgcggatcct gcggtagaga tcgtgctgca cctccgccag
     6001 ctgcgcgtga ccgcggtcga cgacctgata cttcccggcg aagccgagcg ccacgcagag
     6061 atactgcaac tccatcaagt cgatatgccg cgccggatcg ccggagatcc tgtccagcat
     6121 ctcgaagaac ttctccccgc cctgtgcctc ccggtgcagg gcgacgagca gcgtctgttg
     6181 cgcccactcg ctttgcgcgc cccagggagt cgacagcacg gcctcgtcca cccccgcgca
     6241 cagggcgtag cgggcggcga gcaccacctc gctctgaatc cccgacgcgc gcgcgcgatc
     6301 ctcgaaacgc cggatctcgt cgagagcgag acgtctgagc ccgctgatgt cgggggccga
     6361 gagcgacccg cgcagttgcc cggcgagcat gagcagcggg ctggcgacgc ggacgagtgg
     6421 attcagtccg acgccgagac tctcctgcag cgaggatgag atgggttcgg tcgaggcgcc
     6481 cgggcgcggg ccaaacgacg tcgagtctgc gtaaccctgg ggcgagccag gaccacgagt
     6541 cgcggggcta ccagaggggc gccggcctcc tcctggcctc gggcgaagta cggtggcgtc
     6601 agacggcttg aacggatcgt cgttgtccga catcggctcc gtccgtttca gctgcgaatc
     6661 gcccaaaact ccagcgcgag tccgggaaac tcgccggcga cgtgcagcgc cacgccgccc
     6721 gagctcttca actgtcccca gagctcgttc gcctggtcga gctcgaagta cacgaaaccc
     6781 gcatgatacg ggatctggcg cggcgcgacg ggcatcgggt gcacgggaac gcccggcagc
     6841 tgcagattga ccaggtcgcg aatcttctcg acgggcccga tcttgagctg cgtcgggaag
     6901 cgccgccgga gatcctcggc cgcgacgtcg gcgcgcgccg ccaggacgaa gacagcggtg
     6961 ctgtacagcc cgcgatcggc gacggtcgcc acgctgatgc cgaacttctt cgactcgatc
     7021 ggaatcggga tcgccgcctg ctcgagcacc gcgctgagag acgcgcgaag cgccgccatc
     7081 accggctcga acgactcgcg caaacggtcg tgccggtacc cttcgacctt cggggggcgc
     7141 ttggccgccg tggtgaacgt cgagagctcg ccggccgcgg agacgcaaat ccggaagagg
     7201 tcctcgggat gcagcacgcc cgaatcgacg agatgcgcca gaagaggctc gtagcggttg
     7261 atcgcctgca gcatcaggaa gtcggcgatc tcggccgcgg cgccgcggct gctcgccgac
     7321 acgcgcccgc cgagcgcctc gcctcgctgg tgcagcagcc cgagcagctc cgtggtgaac
     7381 gtcgtcagcc ggcgcgacgc gcgcgtgtcg agcacggtcg gaatgaaccc ctcgtcgagg
     7441 acgacgcgcc tgtccgcgcg gcattcggtc acgtgggcga gcggcacgca ggcgtaggcg
     7501 tcggtcacgt cgctggcgaa caggaagcgc gatcgcagcg ccccgacttc gagcacgacg
     7561 gcatcgtggc cgcctgccgt cgagtcgcgc gcatcgagct cgcggatcgc gtgccgcgca
     7621 aggccatcgg caccggactg cgattgacgc tcgacttcgg ccgcgccgga acggcgcacc
     7681 gggaccgcga ggtacaccgt ctgatcgcgg acttgtggac cgatctcgat cggcgccggc
     7741 agcgggtcgt cctcgggcat gcgcatcggc gtgccgtcgg ggaacacgcc cgaggcccgg
     7801 cgcaggccga acttcccgat gctcagcagg tcgggctcga tctccagatc ggagaacccc
     7861 cagctgtgag ggacgagcgc ctgacagcgt gtctcgacgt agcgctcgaa gtagcggtcc
     7921 tgctgctgga agtgctgcgg cctcaggaac agcccttccg accagacgac tttgttatag
     7981 gccgacattc gcgttggcgt cgcgcggctg gcgcatggcg cgactcactt cacggagtac
     8041 tgaatgcgcg cacgctcgac cctgatcgtg gcgttgttcc tgatcggtgc gggaacgacg
     8101 acccgccact gcgcgttccg aatgtcgcga taggccgcga tcatgcccac aaaacgggcg
     8161 tccttcgaca tcgcgacctg cactgtgtgg ttctggcctg gggccagcac gaactcgtcc
     8221 cggccgatga gctcggggcc cagcgccttc ttttcgtcgt cgtacagcgc aaaaaactct
     8281 gccgcggaga acgccgtgtc ggccttgagc tgatacaggc gcacgaccac gggcgaggcc
     8341 cgcccgttgg catccgggtt cgagtcggcc gtcgcggcaa acgtcagatt cgtcgtcgtc
     8401 ggcttgggcg gcgcactggc gcatgcggcc gcgacgagca tggcgacgag ggccgcggcc
     8461 cgcacaacgg cacgatgatg gatcctcata gcatcacccg tacggcccgg tcacggcgtc
     8521 cgggcgcggt cccgcccctt gagccgctcg agctgctcct cgtacgcctt ggcgaactcg
     8581 tctccgaaca gctcgcggaa gctcgcgtca gcgtcagaga ccatatcact aaacttggcg
     8641 cgatactgat cccagtgccg gagctttccg ggcccggcga gcagcgaccc ttttccttga
     8701 cgatcgaact cttcctgcag gcggtcgggg tgaaactccg ccagcatcgc ctcgaaggcc
     8761 acgcggacgc ccgcgagcat ggccatctga tggctgcgga tgtcgtcgaa ggcatcctcg
     8821 aacgccgcga ccggtccgag atacgcgggg ttccgtttga ccagcaggtt gtgaagcgcg
     8881 tcctcgacgt tcgcggagaa cttgagcgga ttgttctggg tgggcttgaa cgtcgtcacg
     8941 cgcagccgga actcgtcctt gatgcgctgg cgggcctgca gaacgtccat gacgcctgca
     9001 acgaccactc gaaggatctg cccgaagctc cgtgcgaact caggcgtgac ggccgacgcg
     9061 tcgagacctg ccccttcgag gagcgcctgg agcacggcat cgcttgacgc ggcggaagag
     9121 ggtttcgcct gaccctgcgc ggcgcgacca gccgccgcac cgtgcgcggg tcgagcctgt
     9181 cgcggcggtt cggcatcggc cggcgatcgg acctgcgccg ggggacggcg agcggccgat
     9241 gcgacgtcca ccgggcccga ctgcaaggga tcgtaatcgt cgggtatgag accggcgccc
     9301 tgagaaggcg gaggttcgga ccgtacaggt tccggcgcct ggtagtgctc ggacaacaac
     9361 gagccggccg ccagatccg
//
LOCUS       DBNGSH010000183         4200 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1372058, whole genome shotgun sequence.
ACCESSION   DBNGSH010000183 DBNGSH010000000
VERSION     DBNGSH010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1372058"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1285
                     /gene="coaA"
                     /locus_tag="AC16CY_04155"
     CDS             356..1285
                     /gene="coaA"
                     /locus_tag="AC16CY_04155"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIR3353003.1"
                     /translation="MSRFIEFTRAEWARLRESTPLPLTEPQLRGLVGINEPMSLGEAA
                     DIYLPLSRLLNLYVAASQSLQRATATFLGSDGPRVPFVIGVAGSVAVGKSTASRVLQA
                     LLASWPSHPQVDLVTTDGFLLPLAVLEARGMIARKGFPESYDLRRLVRFMADVKNGAP
                     EVLAPVYSHLHYDIVPGEYQAVRRPDIVIVEGLNVLQTGGPRDGRPAPMFVSDFFDFS
                     LFVDAAEHDIEQWYIERFATLRETVFQDPSSYFHRYSSLTPEQAVETARGLWRSINLV
                     NLHENIVPTRERAHVILEKGRDHAVRRVRLRRT"
     gene            complement(1286..3835)
                     /locus_tag="AC16CY_04160"
     CDS             complement(1286..3835)
                     /locus_tag="AC16CY_04160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3353004.1"
                     /translation="MSDQVVPSGGVGDDVAGLGVQDAEIAMGRERQPLAVTLFVWAII
                     AAACAVLVLWGPRSLPDPSLFVVLLAASALASSLQLKLPLGSNSFNLSISYSVDFAAL
                     LLIGTELTMLVAGISAGMQSTFGHNGRNPVSRTVFNAAALVLTVKAAGTTFEYLGGHP
                     GVIELKDIARPLVASALVYYIVNTSIVAAAVGLANRRPVWSVWQSNFLWTAPSYYVGA
                     GAAVACVVFWQTKQWWLLPLTAAPVYLTFRSYRMYVDRLASEKRHKEEVLRLHTNTVA
                     ALEAARRSEQRYALAAAGSNDGLWDWDVATDTLYCSDRWKLMIGLSQDNASVSSVDAW
                     LSLVEDEDKQGVWQALRAHLTGQRSHFEHEYRMKHADGSIRWVLCRGIAVRDETGQAL
                     RLAGSQTDITEQRRTRDSLAQAARHDALTDLPNRTLFRELLQRAMAQNMRAPFKTYAV
                     LFIDLDGFKLVNDTHGHVVGDRFLKAIGERLQSQLRPGEALARLGGDEFAVLTQHFDE
                     PREVRAIAERLLRALAEPFPVSGLQLRGAASIGIVIGDPKQRSVDAVLRDADIAMYRA
                     KAAGRGGYELFDPEMHASDLKRLTFETELRRAVEQNDFTVFYQPVVHLKSGRITGLEA
                     LVRWVRADGHVAPPSEFITVAEETGLIVQLTYQVLREACHQMAAWQQMFSRPLDLSVN
                     LSSKLFTRPEFIDQVASAIAGSGLLPGTLRLEIPESVLIDHSDVVDHNFERLRRLKVA
                     VLLDNFGSGYASLSYLQRYRVDALKLDKSLVARMGTPDDDMASVIVKLAGALGMGLIA
                     EGVETAEHAEQLRALDCPHAQGYLFSAPLAASDVTALLSKEFIVQTLPAAS"
BASE COUNT          699 a         1400 c         1403 g          698 t
ORIGIN      
        1 gagcctgcgc gtcggcaccg gggccggcaa gcagcgtggt cagcagcaac gcgacaagca
       61 tccagtcacc tcgataccct cctgttttcg gctgcggctg cggcaggcgt tagggacatc
      121 ttggtccgtg gtcctaagtc cttggtgctt ggtccgtccc tggtcccttg tccgtccctg
      181 gtccgttgtc ctgtcgttct cggtctgcgt gcgtcgctcg tgggcaattg cgtgcacgtc
      241 gagggggccg tcagtcactc ggtcccgcac acgaacctcg gactgaggac caaggaccaa
      301 gaacggacca cggcccaaga accaagcaca aaggacctgc agttagaatg cccccgtgtc
      361 gcgcttcatc gagttcacgc gggccgagtg ggcgcgcctg cgtgagtcga cgccgctacc
      421 gctgaccgag ccgcagctcc gcggcctggt cggcatcaac gaaccgatgt cgctcggcga
      481 ggcggccgac atctacctgc cgttgtcgcg gctgctgaac ctctacgtcg cggcctcgca
      541 gagcctgcag cgggcaacgg caaccttcct cgggtccgac ggcccgcgcg tgccgttcgt
      601 catcggcgtc gccgggagcg tcgccgttgg caagagcacc gcctcgcggg tgctgcaggc
      661 gctgctggcc agctggccca gccatccgca ggtcgacctc gtgacgaccg atggattcct
      721 gctgccgctg gcggtgctcg aggcacgcgg catgatcgcc cgcaaagggt tcccggagag
      781 ctacgacctg cggcggctgg tgcgcttcat ggccgatgtg aagaacggcg cacccgaggt
      841 gctcgcaccg gtgtattcgc atctccacta cgacatcgtg ccaggcgagt accaggccgt
      901 ccgccggccc gacatcgtca tcgtcgaagg actcaacgtg ctgcagacag gcggcccgcg
      961 cgacgggcga ccggcgccca tgttcgtgtc ggacttcttc gacttttcgc tcttcgtgga
     1021 tgctgcggag cacgacatcg agcagtggta catcgagcgg ttcgcgacgc ttcgcgaaac
     1081 ggtgttccag gacccgtcgt cctacttcca ccgctactcg tcactcacgc ccgagcaggc
     1141 ggtcgagacg gcgcgcggcc tctggagatc gatcaacctc gtgaacctgc acgagaacat
     1201 cgtgcctacg cgcgagcgcg cgcacgtgat cctggagaag gggcgggacc acgccgtccg
     1261 gcgggttcgc ctgcggagga cctgatcagg aagccgccgg cagcgtctgg acgatgaatt
     1321 ccttcgagag cagggctgtg acgtcgctgg ccgcgagcgg ggccgagaag agatagccct
     1381 gtgcgtgcgg gcagtccagt gcacgaagtt gctctgcgtg ctctgccgtc tcgacgccct
     1441 cggcgatgag gcccatgccg agcgcgccgg ccagcttcac gatcacgctc gccatgtcgt
     1501 cgtcgggcgt gcccatgcgg gccaccagcg acttgtccag cttcaacgcg tccaccctgt
     1561 agcgctgcag atagctcagc gaggcgtagc cgctgccgaa gttgtcgagg aggacggcga
     1621 ccttgagccg gcggaggcgc tcgaagttgt ggtccacgac gtcggagtgg tcgatgagca
     1681 cgctctcggg aatttcgagc cggagcgttc cggggagcag accgctgccg gcgatggcgc
     1741 tggcgacctg atcgatgaac tccggccggg tgaagagctt cgacgaaagg ttcaccgaca
     1801 gatccagcgg cctggagaac atctgctgcc acgcggccat ctggtggcag gcttcgcgca
     1861 gtacctggta ggtcaactgg acgatgagcc cggtctcctc ggccaccgtg atgaactccg
     1921 acggcggggc cacgtggccg tcggcacgca cccatcgcac cagcgcctcg agccccgtga
     1981 tgcggcccga cttcagatgc acgaccggct gatagaagac ggtgaagtcg ttctgctcca
     2041 cggcccgccg cagttctgtt tcgaacgtca gccgcttgag atcggacgcg tgcatctccg
     2101 gatcgaacag ctcgtacccg cctcgccccg ctgccttggc gcggtacatg gcgatgtcgg
     2161 cgtcgcggag taccgcgtcg accgaccgct gcttcggatc cccgatgacg atgccgatgc
     2221 tggctgcgcc gcgaagctga agaccgctga ccgggaacgg ctctgccagc gcccggagga
     2281 gccgctcggc gatcgcgcgc acctcgcgcg gctcgtcgaa gtgctgcgtc aggaccgcga
     2341 actcgtctcc gcccagccgc gccagcgcct cgccaggccg gagctgcgac tgcagccgct
     2401 cgccgatggc cttcaggaac cgatcgccga cgacgtggcc gtgcgtgtcg ttgacgagct
     2461 tgaacccgtc caggtcgatg aacagcaccg cgtaggtctt gaagggcgct cgcatgttct
     2521 gcgccatcgc gcgctgcagc agctcacgga agagcgtgcg attcggcaga tctgtcagcg
     2581 cgtcgtgccg cgccgcctgc gccagcgagt cgcgagtccg cctctgctcg gtgatgtcgg
     2641 tctgcgagcc cgcgagacgg agggcctggc ccgtctcgtc ccggaccgca atcccgcggc
     2701 agagcaccca cctgatgctc ccgtcggcgt gcttcatgcg gtactcgtgc tcgaagtggg
     2761 agcgctgtcc cgtcaggtgc gcgcgcagcg cctgccacac cccttgctta tcctcgtctt
     2821 ccaccaggct cagccaggcg tccacgctcg agacgctggc gttgtcctga gacaggccga
     2881 tcatgagctt ccagcgatcg gagcagtaga gcgtgtcggt tgcgacgtcc caatcccaga
     2941 gcccgtcatt cgagccggcc gcggccagcg cgtagcgctg ttccgaccgg cgcgcggctt
     3001 cgagtgcggc gacggtgttg gtgtggagcc gaagcacttc ttctttgtgg cgcttctcgg
     3061 aggcgagccg atcgacgtac atgcgatagg accggaacgt caggtagacg ggtgcagccg
     3121 tcaggggaag cagccaccac tgcttcgtct gccagaacac gacacaggcg acggccgcgc
     3181 cggcgccgac gtagtagctc ggcgccgtcc agaggaagtt ggactgccac acactccaca
     3241 ccggcctgcg gttggcaagc ccgaccgccg cggcgacgat cgacgtgttg acgatgtagt
     3301 agacgagggc gctggcgacg agcggccgcg cgatgtcctt cagctcgatc accccgggat
     3361 ggccgccgag gtactcgaac gtggtgcccg cggccttgac cgtgagcacg agcgccgcgg
     3421 cgttgaacac cgtacgcgac acggggtttc ggccgttgtg accgaaggtg gactgcatcc
     3481 ccgcgctgat gccggcgacg agcatcgtca gctcggtgcc gatgaggagc agcgccgcga
     3541 agtccacgct gtaggagatc gagaggttga acgagttgga gccgagcggc agcttcagct
     3601 ggaggctcga ggccagcgcc gaagcggcga gcagcacgac gaagagcgag ggatcgggta
     3661 gcgaccgcgg tccccagagc accagcaccg cgcaggcggc ggcaatgatc gcccacacga
     3721 agagcgtcac cgccagcggc tggcgttcgc gtcccatcgc gatctcggcg tcctgcacac
     3781 ccagtccggc cacgtcgtcg ccgacgccgc cagatggcac aacctggtcg ctcatgattc
     3841 tggtgcctgg tccctcacta ccttcggtcg tccgacgacg aaagtgctct cgtgttggta
     3901 cgtacggacg ccaactacga gaagtgacat ctttctttcc agagcaacgg cgaagccaaa
     3961 cgcaatggga tgaagacagt cacgcggctg tgggtgcgct gcacgccttc gcgcggcatg
     4021 gcgtggctcc gccaccgaat cacggatcag atccgtgatc gcccggtctg aaaatgtcag
     4081 aggttctcgt gcggatcgga aggctgcttg ctggaggtct ttgccgccgc cagcggggta
     4141 gccggcagca ccaggcgtca actagacgat tcgagcgaca accatcgcgc gagtgcacga
//
LOCUS       DBNGSH010000184         2210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4598949, whole genome shotgun sequence.
ACCESSION   DBNGSH010000184 DBNGSH010000000
VERSION     DBNGSH010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4598949"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="AC16CY_04165"
     CDS             <1..493
                     /locus_tag="AC16CY_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353005.1"
                     /translation="TSPADPLIMALEVAMRHASLVVLLIVLSVPASAQTVTLPMDEVV
                     VHHDDAGRVRGRVVEVVPQGLVLIAANQRLTLSVAGVQRIDTRRDSVWNGAVIGALIG
                     GTWCAFVCGQGLDGPGEATLATLFAAGVWGAAGAGIDALRHNQRTLYQRQPSASVAFR
                     LKF"
     gene            577..>2210
                     /locus_tag="AC16CY_04170"
     CDS             577..>2210
                     /locus_tag="AC16CY_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3353006.1"
                     /translation="MRAGLALLPLFLLVADAAGALAQTDVVLVRGRVVAAESGVPHRR
                     ASIGPARRDPRITPVLTDSEGRFVFPATSRLALALLVSKGGYASETVQVPGADDSPPA
                     ELLVRLSRGGAISGRIADSSGAPVFGTEVVARRTDAGGRGARPGTYTAQTDDLGEYRI
                     FGLPAGRYSVSSGQSGIIRNSPAVSRPVVLTLQPQGSSRTIEVKAGDDHGDVNFDVEV
                     LPQKPGTVTLAGAQLEGLRSILGQAGNVGLTGTIVDDFGEPFQGADVQLLAVQYRSGQ
                     RVAMRTGSAVVLGLGRTSDDRGRYRMGGLQPGTYLVVASTGAAASEPDPGRAANLLPV
                     YHPGTPSVDGALPVQIDDGRDTIGIDVTLTMSRGARVSGMAIDESGGAQSGSVRIVAS
                     RRSSAIAFETREMQLSWPGGRFELENVPPGEYVVQVVGEPGFTSPARFGSAYVSVADG
                     GDPPPVTITATPGATLEGRITLEGEPDSRMAQVLVESLPADPDLGPGRSPGFAASADG
                     TFSRKRLHGPTRFALAKAPEGWYLKSFAIGGVDVTDA"
BASE COUNT          329 a          723 c          780 g          378 t
ORIGIN      
        1 gacttcaccg gcagacccgt tgatcatggc cctggaggtg gccatgcgtc acgcgtcact
       61 cgtcgtgctg ctgatcgtgc tgtccgtgcc ggcctcggca cagacggtga cacttcccat
      121 ggacgaagtc gtcgttcacc acgacgacgc cggccgcgtc cgcggccgcg tcgtggaggt
      181 cgttccgcag gggctcgtgt tgatcgccgc caatcaacgc ctgacgcttt cagtggccgg
      241 cgtgcagcgc atcgacactc gacgcgactc ggtgtggaac ggcgccgtca ttggcgcgct
      301 catcggaggg acctggtgcg ccttcgtctg cggccagggg ctcgacggcc ccggcgaagc
      361 cacgttggca acgctctttg ccgccggggt ctggggagcg gcgggcgccg gcatcgatgc
      421 gcttcgccac aaccagagga cgttgtatca gcgccaaccg tcggcgtcgg tggcgttccg
      481 tctcaaattc tgagtcgaac tcagcgccgc ggcgaatacc gctgttcgca ccagcgtagg
      541 cgctttgcgg tatctttgac gtcactagcg cttctcatgc gtgcgggtct cgcactgctc
      601 ccgctgttcc tgctcgtagc cgacgcggcc ggtgcgctcg cgcagacgga cgttgtcctg
      661 gtccgcgggc gcgtcgtcgc cgccgaaagc ggcgttccgc atcggcgagc cagcatcggc
      721 cccgcccgac gcgatcccag gattacgccg gtgctgaccg acagcgaggg ccgcttcgtc
      781 ttcccggcga ccagccgact ggctctcgcc cttctcgtga gcaagggtgg ttacgcgtcc
      841 gagacggtcc aggtccccgg cgccgacgac tcgccgccgg ccgaactgct ggttcgactg
      901 tcgaggggcg gcgcgatctc ggggcggatc gccgattcgt ctggcgcgcc ggtattcggc
      961 acggaggtcg tcgcccggcg taccgacgca ggggggcgcg gtgcccgtcc gggcacatac
     1021 accgcacaga cagacgatct cggcgaatac cggatcttcg gcctgcccgc cgggcgatat
     1081 tcagtgtcct cgggacagtc agggatcatt cgaaactctc ctgcggtttc ccgccccgtc
     1141 gttctgacac tgcagccgca gggttcttcc cggacgatag aagtcaaggc cggtgacgac
     1201 cacggcgatg tcaacttcga cgtcgaggtg ctgccgcaaa agccgggaac agtcaccctc
     1261 gccggagctc agctcgaggg gcttcgctcc atcctcggtc aggcgggcaa cgtcggcctg
     1321 acgggcacga tcgtcgatga cttcggtgag ccgttccagg gcgcggacgt tcaactcctc
     1381 gcggtgcaat acaggtcggg tcagagggtg gcgatgcgca ccggatcggc agttgtgctg
     1441 ggactgggac ggacgagcga cgatcgcggg cgctatcgca tgggcgggct gcagccgggt
     1501 acgtatctcg tcgtggcatc gaccggcgcg gcggcgtccg agcccgaccc cgggcgtgcc
     1561 gcgaatctgc tgccggtgta tcacccgggc acgccgagcg tcgatggagc gctgccagtg
     1621 cagatcgacg acgggcgcga cacgattggc atcgacgtga cgctgacgat gtcacggggt
     1681 gctcgtgtgt cgggcatggc gatcgacgag tcgggcggcg cgcagagcgg ctcggtgcgg
     1741 atcgttgcga gccgccgatc gagtgccatc gcgttcgaga cgagagagat gcagctctcg
     1801 tggcccggcg gcagattcga gctggagaac gtgccacccg gcgagtacgt cgtccaggtt
     1861 gtcggagaac ccggattcac gagtccagct cgatttggat cggcgtacgt ctccgtggcc
     1921 gacggtgggg accctccgcc ggtgacgatc acggcgacgc ccggcgcgac gctcgagggg
     1981 cgcatcaccc tcgagggtga acccgactcg cggatggcac aggtgttggt cgagtcgctt
     2041 ccggccgacc ccgatctggg tccgggcaga tcgccgggct tcgcggcgag cgccgacggg
     2101 acgttctccc ggaagagact gcacggaccg accaggtttg ctctcgcgaa agcgcccgag
     2161 gggtggtacc tgaagtcgtt cgcaatcggc ggcgtggacg tcacggatgc
//
LOCUS       DBNGSH010000185         7564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3919203, whole genome shotgun sequence.
ACCESSION   DBNGSH010000185 DBNGSH010000000
VERSION     DBNGSH010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3919203"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="AC16CY_04175"
     CDS             complement(<1..599)
                     /locus_tag="AC16CY_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HIR3353007.1"
                     /translation="MSKDDEIPDFPTEITPQSKDFGRWYIDVVRRAELADYSPVKGCM
                     VIRPYGYAIWELMQQGLDRRFKATGHVNAYFPLFIPESLLMKEKEHVEGFAPQVAWVT
                     QGGNEQLEERLVVRPTSETIIGTMYAKWVQSWRDLPILINQWANVVRWEKVTRLFLRT
                     TEFLWQEGHTAHETAEEAEEETRKMLAVYKDFAEADLAMP"
     gene            complement(690..2237)
                     /gene="nadB"
                     /locus_tag="AC16CY_04180"
     CDS             complement(690..2237)
                     /gene="nadB"
                     /locus_tag="AC16CY_04180"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR3353008.1"
                     /translation="MTVRGDFLIIGSGIAGLRAAIALAEAGDVVVLTKADPRESNTGY
                     AQGGIAAAIGSDDSPDLHFADTMAAGDGLCVTEAVRVLVTEGPRYVRELIDWGVAFDR
                     DAGGAPALGREAAHSVRRVLHARDAPGREIGRILWEKVQSHPRVRVFDDALAMSLIAT
                     GGVCGGATFVGRDGRLEHVEAERTLVATGGAGQVFRETTNPDIATGDGIAMAFEAGAR
                     VADLEFIQFHPTALSVAGAPRFLLSEALRGEGAWLVNEAGERFMPRYEQAGDLASRDL
                     VARAIVREVEQTGKPVYLSMAHLDPGFVRRRFPTIAQACRQAGLDLATDRIPVSPAAH
                     YVMGGIETDLHGRSSVERLFAAGEAACTGVHGANRLASNSLLEGLVFGARAADAMMAP
                     VAPASLGRTAAESVVPARDGDSAPSVRALRESMWQHAGLVRSADRLQPLARRLSAWCG
                     DLARARTDSPADRELRRLSSLVTVGLLIARAALRREESRGGHYRSDFPRRDDIHWQKH
                     VSDVLIS"
     gene            2257..3360
                     /gene="aroB"
                     /locus_tag="AC16CY_04185"
     CDS             2257..3360
                     /gene="aroB"
                     /locus_tag="AC16CY_04185"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR3353009.1"
                     /translation="MSIDTTADLISVDVITSSRTSPVWIGEGASDSITTLLDRHGVGP
                     KRFIVSNPTVWRLHGARLQAVLGGTDPILISDGERFKNLQSVSRIYEALIRAGADRGS
                     VIIAVGGGVVGDTAGFAAATFLRGISLVHVPTTLLAQVDSAIGGKVGVNHQLGKNLIG
                     AFHQPALVVVDPLLLKTLPRREFRSGLYEVVKYGVIARRDLFDRIDSDIKAILAKDSR
                     ALTPIIVDSCRIKADVVSKDERESGLRRILNFGHTVGHALEAVTDYRRFRHGEAIAHG
                     MLAAADLAVARSALAERERQALVGLIGRLGPLPPVAGLSIRDVIEAMRRDKKVVHGRL
                     HFVIAIEIGATMTVDDVTEEELRAVLLRLGLKS"
     gene            complement(3404..4801)
                     /locus_tag="AC16CY_04190"
     CDS             complement(3404..4801)
                     /locus_tag="AC16CY_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3353010.1"
                     /translation="MSSFPILIVEDKDSLRTMLRHALEGHGHVVLEARDEAEAVQQLG
                     QSRPVVVLTDLKLPVGDGFGVLRAAKELDPDLPVVVMTAYGSIQDAVTAMREGALDFL
                     AKPIDPDHLLLMVERAIAQRRILTEYVLLKEELADRRGAPRLIGEDGKLRQVSQQLHR
                     AAATDATVLLEGESGTGKELFARALHALSPRAEGPFVAINCAAIPEGLLESELFGYEK
                     GAFTGAAQRKPGRFELAHRGTLFLDEIGDLPLALQAKILRAIEEKRFERVGGTQSLHV
                     DVRIVAATNRNLKQRVTERQFREDLYFRLSVFPIQIPPLRERGDDVLILARHFVERFC
                     RDLNKKPLTLSPTAIEDLKAYAWPGNVRELQNCIERAVILTEGDSIHQRHLNLSFRPP
                     APGQSPAPSPWDQIDLSGTLMEALRRVSTEAERRKIQAALRQAGGDKGRAADVLQLGY
                     KALLQKIKTHGIADS"
     gene            complement(4841..5425)
                     /locus_tag="AC16CY_04195"
     CDS             complement(4841..5425)
                     /locus_tag="AC16CY_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353011.1"
                     /translation="MTSRPTRWRLLGPVATLMVLVSICAVFPASGQTLGDAAREAQAR
                     RKTVKSPGKVYTNDNLRSEPPPAQSTPAPPPTTTATPPSQSGVAPGDDKAKAADQSGK
                     DEATWRERVKAERDALARAETFAAALQSQINGLYAEFTACQAPPQCNQISANRQKSMA
                     ELDRVKREVETHTKNIADIQEEARKAGVPAGWVR"
     gene            complement(5457..6353)
                     /locus_tag="AC16CY_04200"
     CDS             complement(5457..6353)
                     /locus_tag="AC16CY_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3353012.1"
                     /translation="MADGAICAGCGSAVPAGRAVCGRCGSRNLEAEAAPAETRSARPM
                     RLAIVAVALAIGGIAIAMLAGSPKPAAPVAASAPGSAATGQGGAATDASNRPEAATGE
                     FSALDASRSGVAAYNTGDIGAATTQFSAAVEADPNNASALNNLGQVLVRTGRAREAIP
                     YFDRAIAIADSVWAYHFNRARAYAELEEWRRAIDGYTTAAKLFPDDYVTQFNLAKARQ
                     AGGDLVGAISAYGRAAELAPEQAEFQLWHGLALERGGRGTDAAAAYRRFLELQPNGPQ
                     AEKVKARLAQLGGGTGGSPVTP"
     gene            complement(6354..>7564)
                     /locus_tag="AC16CY_04205"
     CDS             complement(6354..>7564)
                     /locus_tag="AC16CY_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353013.1"
                     /translation="GPGGVFYQTFDVTLPNMANTVMPESDAIADVLSYFSTTGSVPLK
                     WAFPQYSHFDVNIGAWGDIAAAAPAISKTVGETWSDPAVGQVGTASSPFVVLTGSGFF
                     KRSVQMQANRGGVVAGNTFYMLDAATGLVLDSRSVGSDGKAETVDNCAAINDCRELKN
                     ALQADPVATGPADSRYISKVYIGDLDGKIWRFDVGQGPLGNPIIQQMLSLYTISTGAG
                     SRASDHPVFASMATVSIGNAQQYLFVGTGSELLPRNSVNVQYAMLVILDQGNNGTQTA
                     IIKLEATDGVGGDEKVTSFPAVAGDIVFFTTTTYKNNACLKPDANLYAFTFIGGPAYD
                     TNNSGTLTAADSTKVRTTAGARATAPFIVDQHLVFGTGDTVESFGDPEDYNNGVGQAG
                     VRILSWREVR"
BASE COUNT         1195 a         2584 c         2499 g         1286 t
ORIGIN      
        1 ggcatcgcga ggtcggcctc ggcgaaatcc ttgtagacgg ccagcatctt cctcgtttcc
       61 tcttcggcct cctcggcggt ttcgtgggcc gtgtgcccct cctgccacag gaactcggtc
      121 gtgcgcagga agagccgcgt gaccttctcc cagcgcacca cgttggccca ctggttgatg
      181 agaatcggca gatcgcgcca cgactgcacc cacttcgcat acatcgtgcc gatgatggtt
      241 tccgacgtcg ggcgcaccac gaggcgctcc tcgagctgct cgttcccgcc ttgcgtgacc
      301 caggccacct gcggcgcgaa tccctcgacg tgctccttct ccttcatcag gaggctctcg
      361 gggatgaaga gcgggaagta cgcgttgacg tggccggtcg ccttgaaccg ccggtcgagg
      421 ccctgctgca tcagctccca gatggcgtaa ccatacggcc gaatcaccat gcagcccttc
      481 acgggcgagt agtccgccag ctcggcgcgc cggacgacgt cgatgtacca gcggccgaag
      541 tccttggact gcggcgtaat ctcggttgga aaatccggaa tctcgtcgtc tttggacatg
      601 aaatcagtcg actcccaacg cccgaggccc aacgcccaac ccgccgccac gtggccttgg
      661 gagtttgggt ttgggagttg ggagttgtct caggagatca gcacgtccga gacgtgcttc
      721 tgccagtgta tatcgtcgcg gcgggggaaa tccgagcgat agtgtccgcc gcggctttct
      781 tcacgccgca gggcggcgcg cgcaatcagc aggccgacgg tcaccaggct cgacaggcgg
      841 cgcagttcgc gatccgcggg cgagtccgtg cgggcgcgag ccagatctcc gcaccatgcc
      901 gacaggcggc gcgcgagcgg ctgcagtctg tccgccgatc gaaccagccc cgcgtgctgc
      961 cacatcgatt cgcggagcgc tcgaaccgac ggggcactat cgccgtcgcg cgcggggacg
     1021 accgattcag cggcggttcg gccgagggaa gcaggggcga cgggcgccat catcgcatcg
     1081 gccgcgcggg cgccgaacac caacccctcg agcagcgagt tgctcgcaag ccggttcgcc
     1141 ccgtgcactc cggtgcaggc cgcttcgccg gcggcaaaca ggcgctccac cgaggatcgg
     1201 ccgtgcagat ccgtctcgat cccgcccatc acgtagtgag cggccgggct cacgggaata
     1261 cggtccgtgg ccaggtcgag gccggcctgt cggcaggcct gagcgatcgt ggggaaccgg
     1321 cgccgcacga agccgggatc gagatgggcc atcgacaggt acacgggctt gcccgtctgc
     1381 tcgacctcgc ggacgatcgc gcgcgcgacc agatcccgcg atgcgagatc gcccgcttgc
     1441 tcgtaccgcg gcatgaaacg ctcccctgcc tcgttcacca gccaggcacc ctcgccgcgc
     1501 agcgcttcgg acagcaggaa ccgcggcgca cccgcgacgc tgagcgccgt cggatggaac
     1561 tgaatgaatt cgagatcggc aacgcgcgcg ccggcctcga aggccatggc aatgccgtcg
     1621 ccggttgcaa tgtcggggtt ggtcgtctcg cgaaagacct gccctgcccc gccagtggcc
     1681 acgagcgttc gctccgcttc gacgtgctcg aggcggccgt cgcggccgac gaacgttgcc
     1741 ccgccgcaca cgccgccggt cgcgatgagc gacatcgcaa gcgcatcgtc gaacacgcgc
     1801 acgcgtggat gcgactgcac tttctcccag aggatccgtc caatctcgcg gcccggcgcg
     1861 tcgcgggcgt gcaggacacg ccgcacgctg tgcgccgcct cgcggccgag cgccggcgcg
     1921 ccgccagcgt cacggtcgaa tgccactccc cagtcgatca actcgcgcac gtagcggggg
     1981 ccttccgtga cgagcacgcg taccgcttct gtcacgcaga gaccgtcgcc ggcagccatc
     2041 gtgtcggcga agtgcagatc gggggagtcg tccgatccga tcgcggcggc aatgccgccc
     2101 tgggcgtaac cggtgttgct ctcccgggga tcggccttgg tgagcacgac cacgtcgccc
     2161 gcctccgcga gcgcgatggc agcgcgcagc ccggcgatgc cgctgccgat gatcaggaaa
     2221 tctccgcgca cagtcacagg ctgctatcct atcaacttga gcatcgatac caccgcggac
     2281 ctgatctccg tcgatgtcat caccagctcc cgcacttcgc cggtctggat cggagaaggt
     2341 gccagcgaca gcatcaccac tctgctcgac cggcacggcg tcggcccgaa gcggttcatc
     2401 gtgtcgaacc cgaccgtgtg gcgccttcac ggcgcacggc tgcaggcggt gctcggaggc
     2461 acggatccca tcctcatttc cgacggcgag cgcttcaaga atctccagtc ggtgtcgcgc
     2521 atctacgaag cgctcatccg ggcgggcgcc gatcgcggca gcgtgatcat cgcggtcggg
     2581 ggcggtgtcg tgggcgacac ggcaggattc gctgccgcga cgttcctcag agggatctcg
     2641 ctggtgcacg tcccgacaac gctgctggcg caggtggaca gcgcgatcgg aggcaaggtc
     2701 ggtgtcaatc atcagctcgg gaagaacctg atcggcgcct tccaccagcc ggcgctcgtg
     2761 gtcgtcgatc cgctgctgct gaaaacgctc ccgcgccgtg agttccggtc cgggctctac
     2821 gaggtcgtca agtacggcgt gatcgcccgg cgcgacctct tcgatcgcat cgactcggac
     2881 atcaaggcga tcctcgcgaa ggacagccgg gctctgacgc cgatcatcgt ggactcgtgc
     2941 cgcatcaagg cagacgtcgt gtcgaaggac gagcgagaga gcggccttcg ccggattctc
     3001 aattttggac acactgtagg ccacgcgctc gaggcggtca ccgactaccg ccgattccgg
     3061 cacggcgagg cgatcgcgca cggcatgctg gcagcggccg acctcgccgt cgcgagaagc
     3121 gctcttgccg aacgcgagcg ccaggccctg gtgggactca tcgggcgact gggtccgctt
     3181 cccccggttg ccggcctctc gatccgcgac gtcatcgaag cgatgcggcg cgacaagaag
     3241 gtcgtgcacg ggcggctgca cttcgtcatt gccatcgaga tcggcgcgac gatgacggtc
     3301 gacgacgtca cagaggaaga attacgggcg gtgctccttc gcctggggct caagagttag
     3361 ggactgtccg gccagcagcc ctgaggcctg aggcctgagg ccttcaagaa tcagcgatgc
     3421 cgtgcgtttt gatcttctgc agtagagcct tgtagccaag ctggaggacg tcggctgccc
     3481 gccccttgtc gccgccggcc tgccgcagcg ccgcttggat cttccggcgc tccgcctcgg
     3541 tgctcacgcg gcgcagggcc tccatgagcg tccccgacag gtcgatctga tcccaggggc
     3601 tcggggccgg cgattggccc ggcgccggcg gccggaacga cagattgagg tgacgctggt
     3661 ggattgagtc cccttcggtg agaatcaccg cccgctcgat gcagttctgg agctcgcgga
     3721 cgtttccagg ccacgcgtac gccttcaaat cctcgatcgc cgtcggtgac agcgtcagcg
     3781 gcttcttgtt gagatccctg cagaaccgct cgacgaaatg gcgagccagg atcagcacgt
     3841 cgtcgccgcg ctcgcgcaag ggcgggatct gaatcgggaa caccgacaag cggaaataca
     3901 aatcctcgcg aaactgccgc tcggtcacgc gctgtttcag gttccggttg gtcgccgcca
     3961 cgatgcgcac atcgacatgc agcgattgcg tcccgccgac gcgctcgaac cgcttttctt
     4021 cgattgcccg caggatcttg gcctggagcg cgagcggcag gtcgccgatc tcgtccagga
     4081 agagcgtgcc ccgatgcgcc agctcgaagc ggcccggctt gcgctgcgcg gcgccggtaa
     4141 aggcgccctt ctcgtagccg aagagctccg actcgagcag gccctcggga atggcggcgc
     4201 agttgatggc cacgaacgga ccctccgcgc gcgggctgag cgcgtggagc gcccgcgcga
     4261 agagctcctt gcctgtgccg ctctcccctt cgagcagcac cgtcgcgtca gtcgcggccg
     4321 cccgatgcag ctgctgcgat acctgacgga gctttccgtc ctcgccgatg aggcgcggcg
     4381 ccccgcgccg gtcggcgagc tcctccttca agaggacgta ctccgtgagg atccgccgct
     4441 gagcgatcgc ccgctccacc atcagcagca ggtgatccgg atcgatcggc ttcgccagga
     4501 agtcgagtgc cccttctcgc atcgcggtca cggcgtcctg aatgctgccg tacgccgtca
     4561 tcacgacgac gggaagatcg ggatcgagct ccttggcggc ccgcagcacg ccgaacccgt
     4621 cgccgacggg gagcttcaag tcggtcaaga cgaccaccgg acgcgattga ccgagctgct
     4681 gaaccgcctc ggcctcgtcc cgggcctcga gcacgacgtg cccgtgccct tcgagcgcat
     4741 gcctgagcat ggtccgcagg gagtctttgt cttcgacgat gagtatgggg aaagacgaca
     4801 ttggtgatcg cgaatcacca atcacgaaat tggcaatgga ttaccgaacc cagcctgcgg
     4861 gaacaccggc cttcctggct tcttcctgga tgtcggcgat gttcttggtg tgtgtctcga
     4921 cttccctctt gacgcgatcc agttcggcca tgctcttctg acggttcgcg gaaatctggt
     4981 tgcactgcgg cggcgcctgg caggccgtga attccgcgta cagcccgttg atctggcttt
     5041 gcagcgccgc ggcgaacgtc tccgcgcgtg cgagcgcgtc ccgctccgcc ttcacgcgct
     5101 cacgccacgt ggcttcgtct ttccccgact gatcggcggc tttcgctttg tcgtcccccg
     5161 gcgccacgcc cgactgcgac ggcggagtcg cggtggtcgt cggcgggggc gcgggcgtcg
     5221 actgagccgg cggaggctcg gagcgcaggt tgtcgttcgt gtagaccttc ccgggcgact
     5281 tcaccgtttt tcgcctggcc tgggcttcgc gcgcggcatc acccagggtc tgaccagagg
     5341 cgggaaaaac ggcacagatc gacacgagga ccatcagcgt ggcaacaggg ccgagcagac
     5401 gccaacgagt cggtcgtgag gtcatgagta gattgtcgaa ccggggcgcg ccaccgtcaa
     5461 ggcgtgacag gcgagccgcc tgtaccgccg ccgagctggg cgagcctcgc cttcaccttt
     5521 tcggcttgag ggccgttggg ctgcagttcc aggaagcggc ggtaggcggc ggctgcatcc
     5581 gttccccggc cgccgcgctc gagagccagg ccgtgccaga gttggaactc agcctgctcg
     5641 ggcgcgagct ccgcggcgcg cccgtaggcg ctgatcgcgc ccaccaggtc gccgccggcc
     5701 tgcctcgctt tcgcgaggtt gaactgcgtg acgtagtcgt cggggaacag cttcgcggcg
     5761 gtggtatatc cgtcgatcgc ccggcgccac tcctccagct ccgcgtaggc tcgcgccctg
     5821 ttgaagtgat acgcccagac gctgtcggcg atggcaatcg ccctgtcgaa gtagggaatc
     5881 gcctcgcgcg ctctgccggt gcgaacgagc acctggccga gattgttcag cgcggacgcg
     5941 ttgttgggat cggcctccac cgcggcgctg aactgcgtcg tcgctgcccc gatgtcgccg
     6001 gtgttatacg ccgcgacacc cgagcgggag gcgtcgagcg ccgagaactc tcctgtggcc
     6061 gcctctggtc gattcgacgc gtcagtcgct gcgccgccct gaccggtcgc ggccgatccg
     6121 ggcgccgacg ctgcgaccgg cgccgcaggt ttcggcgatc ccgcgagcat ggcgatggcg
     6181 attccgccga tcgccagcgc gaccgccacg atggcgaggc gcatcggcct ggccgagcgc
     6241 gtctcggccg gagcggcctc ggcctccaga ttgcgcgagc cgcaccggcc gcacacagcg
     6301 cggccggccg gaaccgccga gccgcagcct gcgcagatcg cgccgtcggc cacctatcgc
     6361 acctcacgcc acgacagaat ccggacgccg gcctgtccga cgccgttgtt gtagtcctct
     6421 ggatcaccga acgattccac ggtatcgccg gtgccgaaca ccaggtgctg atcgacgatg
     6481 aacggcgcgg tcgcgcgcgc gcccgccgtc gtgcgcacct tcgtgctgtc ggctgcggtc
     6541 aaggttccgc tgttgttggt gtcgtaggcc ggtccgccga tgaaggtaaa ggcgtagaga
     6601 ttggcgtccg gcttcaggca cgcgttgttc ttgtacgtcg tcgtcgtgaa gaagacgatg
     6661 tcgccggcga ccgccgggaa cgacgtcacc ttctcgtcgc cgcccacgcc gtcggtggct
     6721 tcgagcttga tgatggcggt ctgcgtgccg ttgttgccct ggtcgagaat gacgagcatc
     6781 gcgtactgga cgttgacgct gttgcgcggc agcagttcgc tgcccgtgcc cacgaagagg
     6841 tactgctgcg cgtttccgat gctcacggtc gccatcgatg cgaacacggg gtgatccgag
     6901 gccctggacc cggcacccgt gctgatcgtg tacaggctga gcatctgctg gatgatggga
     6961 ttgcccagcg gcccctgacc cacgtcgaac cgccagatct tgccgtccag atcgccgatg
     7021 tagaccttcg agatgtaccg cgagtccgcg gggcccgtgg ccacagggtc cgcctgcagc
     7081 gcgtttttca gctcccggca gtcgttgatg gcggcgcagt tgtccacggt ctccgctttg
     7141 ccgtcgctgc cgacgctccg ggagtccagc accaggccgg tcgcggcgtc gagcatgtag
     7201 aaggtgttgc cggccacgac cccgccgcga ttggcctgca tctgcaccga cctcttgaag
     7261 aagccggacc cggtcagcac gacgaagggc gacgaggccg tcccgacctg gcccacggca
     7321 ggatccgacc acgtctcacc gacggtcttg gaaatggcgg gagcggccgc cgcgatgtcg
     7381 ccccacgcac cgatgttcac gtcgaaatgc gaatactgcg gaaaggccca tttgagcgga
     7441 acgctgcccg tcgtggagaa gtacgacagc acgtcggcga tcgcgtcgct ctcgggcatg
     7501 accgtgttcg ccatgttggg cagcgtcacg tcaaacgtct gatagaagac cccgcccgga
     7561 ccct
//
LOCUS       DBNGSH010000186         5587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_14238, whole genome shotgun sequence.
ACCESSION   DBNGSH010000186 DBNGSH010000000
VERSION     DBNGSH010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_14238"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="AC16CY_04210"
     CDS             complement(<1..552)
                     /locus_tag="AC16CY_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353014.1"
                     /translation="MIYQGMPLIRRSPAYLGIAILFAGIVTWGFWRSYFAPLVSGTVE
                     RPWIVHLHAAVFVGWVLLLIAQAALASAGRVRLHRQIGVAGMAYGTFVFLIGVMVSIG
                     APALRVRAGDFPVEVGGMVAILSLADLVLFGAFLAFAFAYRSEPEVHKQWVVAATAAL
                     GGAAVGRVLKSDSLAYLLVWLSPV"
     gene            complement(688..1581)
                     /locus_tag="AC16CY_04215"
     CDS             complement(688..1581)
                     /locus_tag="AC16CY_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR3353015.1"
                     /translation="MSTIVVAGGTGFLGTALVRALRGDGHAVTVLTRRPRRDGEVAWA
                     PGESDASWAPMFGGAEVVVNLAGSSIAGGRWTTARKREILESRTQATRALVRACASRP
                     RPSRVFISSSAIGVYGTRGDEPATERTATGSDFLSSVGREWESLALQAAPAARVVLLR
                     TGMVLAREGGALPQLALPFTLFAGGPLGSGRQYMSWIHLHDWIEIVKWAIANPGVSGP
                     LNATAPGAVTNAEFARTLGGVLGRPAWLKTPAFALRLLLGEMADALVLGGQRVVPDAA
                     QRLGFVFRYEALEPALTQIYS"
     gene            1692..2315
                     /locus_tag="AC16CY_04220"
     CDS             1692..2315
                     /locus_tag="AC16CY_04220"
                     /inference="COORDINATES: protein motif:HMM:NF037007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3353016.1"
                     /translation="MVKRRRPARDTRTALLHAAAREFADRGYDAAGTDRIAARARVNK
                     AMLYYHFHSKRGLYLEIVRDMVATLGARIRAIADGPGPAEQKLDRWIGAIVEEAAARP
                     WFPPIMLRELAAGAPHVDAEIFRLVNSVLAGVRDVIAQGQREGAFRDVDPLLAHLSIM
                     PPVLIFFARQRVLAATKLRGGLLEPRNVEEFLCHMQAAARAMLRKPS"
     gene            2318..3316
                     /locus_tag="AC16CY_04225"
     CDS             2318..3316
                     /locus_tag="AC16CY_04225"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3353017.1"
                     /translation="MTRPLVLALPAWFAACTSSLPADTIRVSGHVEATEVRIGAEVGG
                     RIVDLLVAEGDRIDAGTVVARLDTRDTQLQIDRTRAEQASATAQLRLLEAGSRAEDIR
                     QARAQVDAAASEAAAVDAEVKSAQLDLERVQSLLDASAAAPKQRDDAQARVDVAVARQ
                     RAANDRVRAAREALSRLEAGTRPEELHAARARVNAFDAQVAVLEKAIRDADVVSPVAG
                     IVTQKLVDAGELVARGTPLVVVTDLDRAWANLFVPEPTVPRIALGQEATVLTDAGGPG
                     IPGKVTFISSRAEFTPRNVQTAEERSKLVYRIKVSVDNRQGVLKLGMPVDGQISLK"
     gene            3313..4230
                     /locus_tag="AC16CY_04230"
     CDS             3313..4230
                     /locus_tag="AC16CY_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353018.1"
                     /translation="MSAISFTDVVKRYGTTEALSALTLDVRQGEMFGLIGPDGAGKTT
                     AIRLMCGLLHPDSGQIRVLGLDPAKEHRRLTERVGYLSQRFSLYGDLTIDENIAFFAE
                     VHGVRDYARRRDQLLAMTQLTPFRGRLADRLSGGMKQKLALACTLVHEPELIVLDEPT
                     TGVDPVSRREFWKLLAEFLSNGITILMSTPYLDEAERCSRVALVHQGRVLAMDEPARL
                     LTLISGRMLEVIAPGRESARILRSLPHVADAQLFGERVHVTLTDSEEGAEARFVAALR
                     GTPLAGAPVRPIAASLEDVFIARLAGGTT"
     gene            4285..5211
                     /locus_tag="AC16CY_04235"
     CDS             4285..5211
                     /locus_tag="AC16CY_04235"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353019.1"
                     /translation="MNAIEVRRLTRRFGDFVAVDDVSFDVAAGEIFGFLGSNGAGKST
                     TIRMLCGLLRPSSGTARVGGIDVTADPEGVKRRIGYMSQRFSLYEALTVDQNIAFFGG
                     IYGLTRDRLAARRDFVLEMAGLRGREGTLTGALSAGWRQRLALGCAILHEPPIVFLDE
                     PTGGVDPLSRRQFWRLIEDLSERGVAVLVTTHYLDEAEHCQRIAIIQAGRLAAHGTVD
                     ELKGRFASRPILEIRASDAVRAMRALDEMPEVAKTSVFGTSVHAVLRSAEASPEAIAS
                     ALAARGIGVASCGVVAPSLEDVFLDLADRSVA"
     gene            5208..>5587
                     /locus_tag="AC16CY_04240"
     CDS             5208..>5587
                     /locus_tag="AC16CY_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353020.1"
                     /translation="MKSAFWKTVSVAWKELRQIRRDRRTLLILMFVPAFFLLVYGYAL
                     NFDIRHVRLAVQDNDGSTASRELVSAFVNSGYFDLVAEAPDARAIDRLVNRGTVRAVL
                     VIPSRFGTSVETGQPTSVQVLVDG"
BASE COUNT          918 a         1935 c         1874 g          860 t
ORIGIN      
        1 cacgggagag agccacacca acaagtacgc cagcgagtca ctcttgagta cgcggccaac
       61 ggccgcgccg ccaagggccg ccgttgcggc aacaacccat tgtttatgta cctcgggctc
      121 cgaacgatac gcgaaggcaa aggcgaggaa cgcgccgaaa agcaccaagt cggcgaggct
      181 gagaatcgcc accatgcctc cgacttcgac cgggaaatct ccggctcgta cgcgaagcgc
      241 cggcgccccg atactgacca tgacaccgat gagaaagacg aacgtaccgt acgccattcc
      301 agccacaccg atctgtcggt gtaagcgcac gcgcccggca ctcgcgagtg ccgcctgggc
      361 aatcagtaat aacacccagc cgacaaatac cgccgcatgc agatgaacga tccatggtcg
      421 ctcgacagtg ccagaaacca gcggagcgaa atagcttcgc cagaaacccc aggtgacgat
      481 tccggcgaac agaattgcga tcccgaggta ggccggactt cgtcgtataa ggggcatccc
      541 ttggtagatc atgctgtact cgctaaagaa aacacatgcc gaaaggggac acgccccgcc
      601 gatcgcccag tgaaccttgg ctcgagtggg tcagtgaacc acaaaccagg gaggcacgga
      661 gacgtgcctc agagctccgt ggttcgttca ggaatagatc tgcgtgagcg cgggttccag
      721 cgcctcgtac cgaaagacga agccgagcct ctgcgccgcg tccggcacca cgcgctgacc
      781 gccgaggacc agcgcgtcgg ccatttctcc caggagcagc cgcagtgcga acgcgggtgt
      841 tttcagccac gcagggcgcc ccagaacgcc gccgagcgtt cgcgcgaact cggcgttggt
      901 cacggcgccg ggtgcggttg cattgagcgg acccgacacg ccgggattgg cgatcgccca
      961 cttcacgatc tctatccagt cgtgcaggtg aatccacgac atgtactgcc gccccgatcc
     1021 gagcggcccg cccgcgaaca gcgtgaaggg gagcgccagt tgtggaagcg cgcctccctc
     1081 gcgagcgagg accatgcccg tgcgcaacag gacgacgcgc gcggccggcg cggcctggag
     1141 cgccagcgac tcccactcgc ggccgacgct cgacaggaaa tcggagcccg tggccgtgcg
     1201 ctccgtggcc ggctcgtcgc cgcgcgttcc gtagacgccg atcgcggagc tgctgatgaa
     1261 cacacgggac ggacgcggcc tggacgcgca ggcgcgcacc agcgcgcgtg tcgcctgcgt
     1321 gcggctctcg aggatctcgc gcttgcgcgc ggtggtccag cggcccccgg cgatggagga
     1381 gccggcgagg ttgacgacga cctcggcgcc gccgaacatc ggcgcccagg acgcatcgct
     1441 ttcacctggt gcccaggcca cctcgccatc ccgtcttggg cgtctcgtca acacggtgac
     1501 agcgtggccg tcgccgcgaa gggcccgcac gagcgcggtg ccgagaaacc cggtgccgcc
     1561 ggcgacgaca atggtggaca tctcaggctc cggtctccag tctacggttt ccggcgcctt
     1621 gcgggcggcc tgctgcccgc cgccgggagc tccttgactc cccgtgcccg gtgttgtaac
     1681 taactaacta gttggttaaa agacgacggc ccgcccgcga cactcgtacg gcgctcctgc
     1741 acgccgccgc gcgggagttt gccgaccggg gctacgacgc cgccggaacc gaccgcatcg
     1801 ctgcccgcgc ccgcgtcaac aaggcgatgc tctactacca cttccacagc aaacgcggcc
     1861 tctacctcga gatcgtgcgg gacatggtgg ccacgctcgg cgcgcgcatc cgtgcgatcg
     1921 ccgacgggcc gggacctgcc gagcagaagc tcgatcgctg gatcggcgcc atcgtcgaag
     1981 aggccgccgc ccggccgtgg tttccgccca tcatgctgcg cgagctggcg gccggcgcgc
     2041 cccacgtcga cgccgagatc ttccgcctgg tcaacagcgt tctggccggc gtgcgcgacg
     2101 tgatcgccca agggcagcgc gagggtgcct tccgcgacgt cgatccgctg ctcgcacacc
     2161 tgtcgatcat gcctcccgtg ctcatcttct tcgcccgcca gcgtgtgctg gcggccacca
     2221 agctccgcgg cggcctcctc gaaccgcgca acgtcgagga gtttctctgc cacatgcaag
     2281 cggccgccag ggccatgctc cggaaaccct catgaacatg acacgcccgc tcgtcctcgc
     2341 tctgcccgcg tggtttgccg cgtgcacgtc ctcgctgcca gccgacacga ttcgcgtgtc
     2401 cggccacgtc gaagcgacgg aggttcgaat tggagcggag gtcggcggcc ggatcgtcga
     2461 tctgctcgtg gcagagggcg accgcatcga cgcaggcacg gtcgtcgcgc ggctcgacac
     2521 acgtgatacc cagcttcaga tcgaccggac gcgcgccgag caggcgtcgg ccaccgcgca
     2581 gcttcgtctg ctcgaggccg ggtcacgcgc cgaagacatc cggcaggcgc gagcgcaggt
     2641 ggacgcggcg gcctctgagg ccgctgccgt cgacgcggag gtgaaatcgg cgcagctcga
     2701 tctcgagcgc gtgcagtccc tcctcgacgc cagcgcggcg gccccaaaac agcgcgacga
     2761 cgcccaggcg cgggtggacg tggcggtcgc gcggcagcgt gccgcgaacg accgcgttcg
     2821 agcggcgcgc gaggcgctgt cgcgcctcga agcaggcacg cggcccgaag agcttcacgc
     2881 cgcccgcgcc cgcgtgaatg ccttcgacgc gcaggtcgcc gtcctcgaga aggcgatccg
     2941 ggatgcggac gtggtgtcac ccgtcgcggg gatcgtcacc cagaagctcg ttgacgccgg
     3001 cgagctcgtg gctcgcggca cgcctttggt cgtcgtcacc gacctcgatc gtgcctgggc
     3061 caatctgttc gtacccgagc cgacggtgcc ccgcatcgcg cttgggcagg aagccaccgt
     3121 gctcaccgac gccggaggcc cgggcatccc cgggaaggtc accttcattt cctcgcgcgc
     3181 ggagttcacg ccacgcaacg tccagacagc cgaggaacgc tcgaagctcg tgtaccggat
     3241 caaggtgagc gtcgacaacc gccagggcgt cctcaagctg gggatgcccg ttgacggaca
     3301 gatcagcctc aagtgagcgc catctccttc accgacgtcg tcaagcggta cggcacgacc
     3361 gaggcgctct cggccctgac actcgacgtc cggcagggcg agatgttcgg cctcatcggt
     3421 cccgacggtg cgggcaagac gacggccatc aggttgatgt gcgggctgct gcatcccgac
     3481 tcggggcaga tccgcgtgct tggcctcgat cccgccaaag aacaccggcg cctgaccgag
     3541 cgggtcgggt acctgtcgca gcggttcagc ctctacggcg acctcacgat cgacgagaac
     3601 atcgcgttct tcgccgaggt gcacggcgtg cgcgattacg cgcgacgccg cgaccagctt
     3661 ctcgccatga cgcagctgac gccgtttcgc ggacggctgg ccgatcggtt gtcgggaggc
     3721 atgaagcaga agctcgcgct cgcatgcacg ctcgttcacg agcctgagtt gatcgtgctc
     3781 gacgagccga cgacgggcgt ggaccccgtg tcgaggcggg aattctggaa gctgctggcg
     3841 gagttcctgt ccaatggcat cacgatcctc atgtccacgc cgtacctgga cgaggcggaa
     3901 cgctgctccc gtgtcgcgct cgtacaccag gggcgcgtcc tcgcgatgga cgaaccggcg
     3961 aggctgctca cgctgatatc cggccggatg ctcgaagtga tcgcccccgg gagggagtct
     4021 gcgcggattc tgaggagcct gccgcacgtc gctgacgcgc agctcttcgg cgagcgggtg
     4081 cacgtcaccc tgaccgacag cgaagaaggc gccgaagcgc gcttcgttgc ggcgcttcgg
     4141 ggcacgccgc tcgcaggggc gccagtgcgg ccgatcgccg catcgctcga ggacgtgttc
     4201 atcgcgcgac tggcgggggg caccacataa gggctcaggg ctcagggctc agggctcagg
     4261 gctcaggact cagggcgtaa ggcgatgaac gcgattgaag tacggcggct gaccaggcgc
     4321 ttcggcgatt tcgtcgccgt ggacgacgtg tcgttcgacg tcgcggcagg ggagatcttc
     4381 ggctttctcg gcagcaacgg cgccggcaag tccacgacca tccgcatgct gtgcggcctg
     4441 ctgcggccct cgtcgggtac cgcccgcgtc ggcggcatcg acgtcacggc cgatcccgaa
     4501 ggcgtgaagc ggcgcatcgg ctacatgtcg cagcgcttct cgctgtacga ggcgctcact
     4561 gtcgatcaga acatcgcgtt cttcggcggc atctacgggc tgacgcgcga tcgtctggcc
     4621 gctcgtcgcg acttcgtgct cgagatggcc ggcctgcgcg gccgtgaagg aaccctgacc
     4681 ggcgccctct ctgccggctg gcgccagagg ctcgcgctcg gctgcgcgat cctccacgag
     4741 ccgccgatcg tgtttctcga cgagccgacc ggcggcgtcg atccgttgtc gcgccggcag
     4801 ttctggcgtc tcatcgagga cctgtccgaa cgcggcgtcg ccgtgctggt gacgacgcat
     4861 tacctggacg aagccgagca ctgccagcgc atcgccatca tccaggcggg gcgtctggcc
     4921 gcgcacggca cggtggacga gctgaagggc cgctttgcct cgcgcccgat tctcgagatc
     4981 cgcgcctcgg atgcggttcg ggccatgcgc gcgctcgacg agatgccgga ggtcgccaag
     5041 acgagcgtct tcgggacctc cgtccatgcc gtgctccgat cggcggaggc cagtcccgag
     5101 gcgatcgcct cggcactcgc ggcgcgcggc atcggcgtcg cgtcgtgcgg cgtggtggcg
     5161 ccgtcgctcg aggacgtctt tctcgacctc gccgacagga gcgtcgcgtg aagagcgcgt
     5221 tctggaagac ggtgtcggtg gcctggaagg agctccgtca aatccggcgg gatcgccgga
     5281 cgctgctcat cctgatgttc gtgccggcgt tcttcctgct cgtctacggc tacgcgctca
     5341 acttcgacat ccgccacgtc cggctcgccg tgcaggacaa cgacggcagc acggccagcc
     5401 gcgagctcgt gtcggccttc gtcaactcgg gctacttcga cctagtggcc gaggcgcccg
     5461 acgctcgagc gatcgaccgg ctcgtcaatc gcggcacggt ccgcgccgtg ctggtgatcc
     5521 cctcgcgctt cggcacgagc gtggagacgg ggcagccgac gagcgttcag gtgctggttg
     5581 acgggga
//
LOCUS       DBNGSH010000187         3925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_14304, whole genome shotgun sequence.
ACCESSION   DBNGSH010000187 DBNGSH010000000
VERSION     DBNGSH010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_14304"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="AC16CY_04245"
     CDS             complement(<1..579)
                     /locus_tag="AC16CY_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3353021.1"
                     /translation="MVACLAMASMATAAEPTLLDAAERGDRAAALRLLERGANPNTPG
                     PDGTTAIMYAAANDDIELVRALIKAGANAGLKNQFGTSAITEAAIIGSASVIDALLQA
                     GADLNESLPVRRRGPQAAAGQPAPPVSNEVGLNAFLLAAANAHYELAARLLDRGADPN
                     KGVTGQLHSTSLCCGDYANGSAFFRAAIASDVE"
     gene            complement(659..1945)
                     /locus_tag="AC16CY_04250"
     CDS             complement(659..1945)
                     /locus_tag="AC16CY_04250"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3353022.1"
                     /translation="MHVFKKHVSRRTVLKGVGATIALPLLDAMNPAATAWAQTAAGST
                     PKRLAFVGFPHGAIMDRWSPPQTGTNYEMSPILQPLESLRQHLTIVSGLRNKPGETPE
                     PHGYIEMTWLTCVKPWDRGVAGPDSGVSADQFAARHIGQDTRVPSLELTTSMGGARLA
                     WRTPTQPLPQEGNPRAVFQKLFGQGDTDVERAAILHETGSILDRVKGQAARLQASLGV
                     ADRVVVSDYLDSVREIERRVQMASQQDMSRLDIPNAPVGTPNDLTEHFKLMFDLMALA
                     FQADITRVITLSMDREASMRTYTNLGIAEGFHPLSHHGNNPQKQEKLVQIQRYHTEVF
                     ATFVERLSKAKEGDGTVLDHSTILYGSNMSNSDKHNNDPLPSAILGHAHGRIKGGQHL
                     KYPQDSRFADLLVTLFDRNNIPVEKLGDSGGIFSEI"
     gene            complement(2052..>3925)
                     /locus_tag="AC16CY_04255"
     CDS             complement(2052..>3925)
                     /locus_tag="AC16CY_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3353023.1"
                     /translation="GSQLTHVPGLPLGTRGGILANVDLPSDGEYVVDIADMATHIWGN
                     GMEFENPLVVTLDNKVVYETVIGGEEDMKLYDQVQNGALDRVNARLKNIKFFATAGPH
                     KIGVAFKRRTFAESDDIQQMFAAGGGQDRLYRVASFQVRGPFDAKGIGSTPSRDRIFT
                     CRPKSDDVERGLQPTRQVERGLQPPRREDEAACARQIISTLARRAYRRPVTAEDVNEL
                     YQYYQDGAKDGFEPGIRSAITGLLASPFFLYRGERVPAGLRPGDSYAIGDLELASNLS
                     FFLWNTIPDEELLQLAIDGKLRTPAVLDRQVKRMLADPRSLTLASNFVHQWLDMKRLD
                     EIVPDSAVFPYASGRSDPREDFRTELTLFADSVFREDRSVVDLLRAPHTFLNERVALH
                     YGITDVKGARFRRVELADSVRWGLLGKGAVLMAAAYPNRTSPVLRGAFVLKHIQGVPP
                     ALPPNDLQIPTLDEKDVGTTKARTVREMIATHRANPVCASCHAVMDPLGLALENFDAT
                     GKWRDRDRYAGTLIDASTALPDGTKITGPDDLRAALLRRPEQFAQTFTEGLMTYATGR
                     KLEHYDMPTVRGIVRGAAAGDYRFSALVQAVVRSEQFRMRRVPQPVDGGARVAANAN"
BASE COUNT          582 a         1307 c         1353 g          683 t
ORIGIN      
        1 ctcgacgtcg gaggcgatgg cggcgcggaa gaacgccgaa ccattggcgt agtcgccgca
       61 gcagagggac gtggagtgca gctgcccggt gacgcctttg ttcggatcgg caccgcggtc
      121 gagcagccgt gccgccaatt cgtaatgggc gttggcggcg gcgagcagga acgcgttgag
      181 gccgacttca ttcgagaccg gcggcgccgg ctggccggct gctgcctgcg gaccgcgtcg
      241 tctcaccggc agcgattcgt tcaggtcggc gcccgcctgg agcagcgcgt cgatgacgga
      301 cgcggagccg atgatggcgg cctcggtgat ggcggaggta ccgaactggt tcttcaggcc
      361 ggcgttcgcg cccgccttga tcagcgcgcg gacgagctcg atgtcgtcgt tggccgcggc
      421 gtacatgatc gccgtggtcc cgtcaggccc gggcgtgttc gggttggcgc cccgttccag
      481 caggcgcagc gcggccgcgc ggtcgccgcg ctcggcggcg tcgagcagtg tcggctccgc
      541 ggcagttgcc atcgaggcca tggccagaca cgcaaccatc acgtagagcg cgggctttag
      601 cccgcgcgga cacgcgcacg ctgaagcgtg cgctctacgg agccggaatg aatccacctt
      661 agatctccga gaagattccg ccgctgtcgc cgagcttctc caccgggatg ttgttgcgat
      721 cgaacagcgt caccagcagg tcggcgaagc gcgagtcctg cgggtacttc aggtgctggc
      781 cgcccttgat gcgcccgtgc gcgtggccga ggatcgccga cggcagcgga tcgttgttgt
      841 gcttgtcgct gttgctcatg ttgctgccgt acaggatcgt cgagtggtcc agcaccgtgc
      901 cgtcgccctc cttcgccttc gacagccgct cgacgaacgt cgcgaacacc tcggtgtggt
      961 accgctgaat ctggacgagc ttctcctgct tctgcggatt gttcccgtga tgcgagagcg
     1021 ggtggaaccc ctcggcgatg ccgaggttcg tgtacgtccg catgctcgct tcgcggtcca
     1081 tcgacagcgt gatgacgcgc gtgatgtcgg cctggaacgc cagcgccatg agatcgaaca
     1141 tcagcttgaa gtgctccgtg agatcgttcg gcgtgccgac cggcgcgttc gggatgtcca
     1201 gccgggacat gtcctgctgc gacgccatct gcacgcggcg ctcgatctcg cggacggagt
     1261 cgagatagtc gctgacgacg acgcgatcgg cgacgccgag gctcgcctgc aggcgcgccg
     1321 cctgtccctt gacgcggtcc aggatgctgc cggtctcgtg aagaatcgcg gcgcgctcga
     1381 cgtcggtgtc gccctggccg aagagcttct ggaacaccgc gcgcggattg ccttcctgcg
     1441 gcagcggctg cgtcggcgtc cgccacgcca gccgcgcgcc ccccatcgac gtcgtcagct
     1501 cgagcgacgg cacccgcgtg tcctggccga tgtgcctcgc ggcgaactga tcggcgctga
     1561 cgcccgagtc gggcccggcg acgccgcggt cccacggctt cacgcacgtc agccacgtca
     1621 tctcgatgta accgtgcggc tcgggcgtct cgcccggctt gttgcgcagc ccggacacga
     1681 tcgtcaggtg ctggcgcagc gactccagcg gctgaaggat cggcgacatc tcgtagttgg
     1741 tgccggtctg cggcggcgac catcgatcca tgatcgcgcc gtgcgggaag ccgacaaacg
     1801 cgagccgctt cggcgtcgag ccggcggcgg tctgcgccca cgcggtggca gccggattca
     1861 tcgcatcgag caggggaagc gcgatggtgg cgccgacgcc tttcaacacc gtccgccgcg
     1921 agacgtgctt cttgaagacg tgcatgtcag atctccgtgt tcacacttct cggttctcgc
     1981 ttctcagttt ccgccgtcgc caaggctttg gcgagacccc gccgaagcgc ttcgcgcgaa
     2041 ggcgggcgtg ttcagttcgc gttcgcggcc actcgagctc ctccgtccac cggttgcggc
     2101 acccgtcgca tcctgaactg ctcgctccgc acgaccgcct ggaccagcgc cgagaaccgg
     2161 tagtcgcccg ccgccgcgcc acggacgatg ccgcgcacgg tgggcatgtc gtaatgctca
     2221 agtttacgcc cggtcgcgta tgtcatcagc ccctcggtga aggtctgggc aaactgctcc
     2281 ggccggcgga ggagcgccgc ccgaaggtcg tccggaccgg tgattttcgt cccgtcgggc
     2341 agcgccgtcg aggcgtcgat cagggtgccg gcgtagcggt cgcgatcgcg ccacttgccg
     2401 gtcgcatcga aattctcgag cgccagaccg agcggatcca tgaccgcgtg gcacgacgcg
     2461 cagaccgggt tggcccggtg cgtcgcgatc atctcgcgga ccgtgcgcgc cttcgtcgtg
     2521 ccgacgtcct tctcgtcgag cgtcgggatc tggaggtcgt tgggcggcag cgcgggaggc
     2581 acgccttgaa tgtgcttgag gacgaacgcg ccgcgcagca ccggtgacgt ccgattgggg
     2641 taggcggccg ccatcagcac ggcacccttg ccgagcagcc cccaacgcac cgagtccgca
     2701 agctcgacgc gccggaagcg ggcgcctttc acgtcggtga tgccgtaatg cagcgcgacg
     2761 cgctcgttga ggaacgtgtg cggggcccgc agcaggtcca cgacgctgcg gtcttccctg
     2821 aagacgctgt cggcgaagag cgtcagctcg gtgcggaagt cctcgcgcgg atccgatcgg
     2881 cccgaggcat acgggaacac cgccgagtcc ggcacgatct cgtccaggcg cttcatgtcg
     2941 agccactggt ggacgaagtt gctcgccagg gtcagcgaac gcgggtcggc gagcatgcgc
     3001 ttcacctgcc ggtcgagtac ggcgggcgtg cggagcttgc cgtcgatggc gagctgcagc
     3061 agctcttcgt cggggatcgt gttccagagg aagaacgaca ggttcgacgc cagctcgaga
     3121 tcgccgatcg cgtaggagtc gcccgggcgc agccccgcgg ggacgcgctc gccgcggtac
     3181 aggaagaacg ggctcgccag caggcccgtg atcgcgctgc ggatgccagg ttcgaagccg
     3241 tccttcgcgc cgtcctggta gtactggtac aactcgttca cgtcttctgc ggtgacaggc
     3301 cggcgataag cacgcctcgc caaggtcgag atgatctgcc tcgcacacgc cgcttcgtcc
     3361 tctcggcgcg ggggctgaag cccccgctca acttgccgcg tgggctgaag cccgcgctct
     3421 acatcatcgg acttgggacg gcaggtgaag atccggtctc ggctcggcgt cgaaccgatg
     3481 cccttcgcgt cgaagggccc acgaacctgg aacgatgcca cgcgatacaa acggtcctgg
     3541 ccgccgcctg ccgcgaacat ctgctgtatg tcgtcggact cggcgaaggt ccgccgcttg
     3601 aaggccacgc cgatcttgtg cggcccggcg gtggcgaaga acttgatgtt cttcaggcgc
     3661 gcgttgacgc gatcgagcgc gccgttctgc acctggtcgt agagcttcat gtcttcctcg
     3721 ccgccgatca ccgtctcgta gaccaccttg ttgtccagcg tgaccacgag cgggttctcg
     3781 aactccatgc cgttgcccca gatgtgggtg gccatgtcgg cgatgtcgac gacgtattcg
     3841 ccgtcggacg ggagatcgac gttggcgaga atgccgccgc gcgtgccgag cggcaggccc
     3901 ggcacgtgcg tcaactgtga gcccg
//
LOCUS       DBNGSH010000188         4474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3239890, whole genome shotgun sequence.
ACCESSION   DBNGSH010000188 DBNGSH010000000
VERSION     DBNGSH010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3239890"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1076
                     /locus_tag="AC16CY_04260"
     CDS             57..1076
                     /locus_tag="AC16CY_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3353024.1"
                     /translation="MIIDSHGHVTAPDSLYVYKAQILAHRGAHGRGGNLANDEDIRKA
                     LNSPVFGGSSHLDQLKEVGTDLQLVSPRPYQMMTSEHPRLVQWFVEETNNIIARQVQL
                     YPHTFRGVCGLPLTPGVSPKAVVPYLEKCVREQGFVGCLLNPDPGECSGVETPALGDR
                     FWYPLYEKMVELDIPGHIHSAGCRSDRLPYSLHFINEESIGVVSLLNSDVFKDFPSLK
                     ILVSHGGGAIPYQFARFEASSLRGKGPRFSEKMRHLYYDTVLYSKDALELLFKTVGVD
                     RCLFGTERPGVGTVKDPRTGRWLDETRFTIEAIEWLSAADKQMIFEDNAKKVFNLKVP
                     EKAVA"
     gene            1076..2113
                     /locus_tag="AC16CY_04265"
     CDS             1076..2113
                     /locus_tag="AC16CY_04265"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3353025.1"
                     /translation="MLRLLVLAPFIAAVGLAGVPSAQAPQKVTINYPTRSGASWHLYL
                     AKEGGYYQKYGLDVELQFGVHPTGIAMLTSNQAVMVNHSLEQGMLAGVRDANAFTLMG
                     STSNKGLFALVGQKGQTFKQLKGKRIAIGQIGDAPYNYTVALLGTIGLGVRDVQWIPV
                     GTDVSGRAAALQTNRADATLLTAPNYFRLEEAGYPILANLADHPVFAATVYLFARKAT
                     VDNPKLAESLIKAHAEAIKRFYDDKAFAVKTYIAFDKQPEADVARIYDLYKQGNIFER
                     VPYVMGGAVSSIVAQQADPRLAADMKAFDFHKVVDNSVVDRLVKEGFFQQLFGPGVRA
                     EEQSKAKLAFR"
     gene            2244..3041
                     /locus_tag="AC16CY_04270"
     CDS             2244..3041
                     /locus_tag="AC16CY_04270"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353026.1"
                     /translation="MHKLEIDGLTKRYYVERDGRQVLALSAVSLSVAEGEFVAIVGPS
                     GCGKTTLLNIVAGLLPYDLGTVAIDGRRVEGPGVDRAVVFQHSSLLPWRTIAGNVRYG
                     MELHRRVDRATMQQRTDYFIGLVGLTGFERHYPSELSGGMQQRVNLARALASDPVVLL
                     MDEPFAALDAQTREQMQVELLKIWTHARKTVLFITHQIDEAVYLADRVAVMSARPGQI
                     KGVFDVPFPRPRPLSVKRDPGFAALEDAIWGLVDARQERGDSVGRPA"
     gene            3038..3847
                     /locus_tag="AC16CY_04275"
     CDS             3038..3847
                     /locus_tag="AC16CY_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353027.1"
                     /translation="MSGTREPELIEAPRRSFYQRHERLILGGLSVVVVLAVWQAFWSA
                     GRISPLFFTGPSAVVKRFAEEWTGGRLKADLVYSGTNFVIGVGLAIATGVVCGVLIGW
                     YRRLGMVFEPFVAALYSTPRVAMIPLILIWFGIGMWSKVFIVFINAFFPVLINTIGGV
                     RAIDSDLLRAARAFCASDWQIFTTVVIPGSVPFILTGVRQAVALGLIGVVVGEMFGGS
                     EGIGYMTNYSGQTFQTDAVFLGVVLIACAGIVLTWLAERLERHFSRWRPER"
     gene            3897..>4474
                     /locus_tag="AC16CY_04280"
     CDS             3897..>4474
                     /locus_tag="AC16CY_04280"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase
                     family protein"
                     /protein_id="HIR3353028.1"
                     /translation="MAEIVLGIGSSHGPLLSTPPEQWDLRAKADRENKGHWFRGKTYD
                     FNTLLAARAPGFAHEITVEKRRERFFKCRQALEALGVKFNQVSPDAVVILGNDQREFF
                     NAGLTPSITVYRGSQISNVQHLNEDAPGLNIAEPANSPSEGATYPGATALADHILDSL
                     ADENFDLAQSDQTPSGAPRGGIPHAYVFFYHT"
BASE COUNT          773 a         1450 c         1501 g          750 t
ORIGIN      
        1 ggcagggcga ggacacctgc tgatacaatc cggctccaca ccacgcggag gcagcggtga
       61 tcatcgactc gcacggacac gtgacggcgc ctgacagctt gtacgtctac aaggcgcaga
      121 tcctcgccca tcgcggggcg cacgggcgcg gcggcaacct ggccaacgac gaggacatca
      181 ggaaggcgct caactcgccc gtcttcgggg gcagctcgca cctggatcag ctcaaggaag
      241 tggggaccga tctgcagctc gtgtcgccgc ggccctacca gatgatgacg agcgagcacc
      301 cgcgactggt ccagtggttc gtcgaggaga cgaacaacat cattgccagg caggtgcagc
      361 tctacccgca caccttccgc ggcgtgtgcg gcctgccgct gacgccgggc gtcagcccga
      421 aagcggtcgt cccgtacctg gaaaagtgcg tcagggagca gggcttcgtc ggctgcctgc
      481 tcaatcccga ccccggcgag tgcagcggcg tcgagacgcc ggccctcggc gaccggttct
      541 ggtacccgtt gtacgagaag atggtcgagc tcgacatccc cggccacatc cattcggccg
      601 gctgccgctc cgatcggctg ccgtattcgc tgcacttcat caacgaagag agcatcggcg
      661 tcgtgtcgct gctcaactcc gacgtgttca aggactttcc ctcgctgaag atcctcgtgt
      721 cgcacggcgg gggcgccatt ccgtaccagt tcgcacggtt cgaggcgagc tcgctccgcg
      781 gcaagggccc gaggttctcg gagaagatgc ggcacctgta ctacgacacg gtgctctact
      841 cgaaggacgc gctcgagctg ctgttcaaga cggtcggcgt ggatcgctgc ctgttcggca
      901 ccgaaaggcc cggcgtgggc acggtcaagg atccacgcac gggacgatgg ctcgacgaga
      961 cacggttcac aatcgaggcg atcgagtggc tgagcgccgc cgacaagcag atgatcttcg
     1021 aggacaacgc gaagaaggtc ttcaacctga aggtgcccga aaaggcggtc gcgtagtgtt
     1081 acgccttctt gtcctggcgc ccttcattgc agccgtcggc ctggctggtg tgcccagcgc
     1141 ccaggctccg cagaaggtca ccatcaacta cccgacgcgg agcggcgcct cctggcatct
     1201 ctatcttgcc aaggagggag gctactacca gaaatacggc ctcgacgtgg agctgcagtt
     1261 cggcgtgcac ccgaccggca tcgcgatgct cacgagcaat caggccgtga tggtgaacca
     1321 ctcgctcgag caggggatgc tggccggcgt ccgcgatgcc aatgcgttca cgctgatggg
     1381 cagcacgtcg aacaaggggt tgttcgcgct cgtggggcag aaggggcaga ccttcaagca
     1441 gctgaagggc aagcgcatcg cgatcggcca gatcggcgac gcgccgtaca actacaccgt
     1501 cgcgctcctc ggcacgatcg ggctcggggt gcgcgacgtc cagtggattc cggtcggcac
     1561 cgacgtcagc ggccgcgcgg cggcgctgca gaccaacagg gcggacgcga cgctcctgac
     1621 ggcgcccaac tacttccggc tggaagaggc cggctatccg attctggcca acctggccga
     1681 ccacccggtc ttcgcggcaa cggtctatct gttcgcgcgg aaggccaccg tggacaaccc
     1741 gaagctcgcc gagtctctca tcaaggcgca cgccgaggcg atcaagcgct tctacgacga
     1801 caaggcgttc gccgtgaaga cctacattgc cttcgacaag cagccggagg ccgacgttgc
     1861 ccgcatctac gacctctaca agcagggcaa catcttcgaa cgcgtcccgt acgtgatggg
     1921 gggcgccgtc tcctcgatcg tcgcgcagca ggccgatccg cgcctcgcgg ccgacatgaa
     1981 ggccttcgac ttccacaagg tcgtcgacaa cagcgtcgtc gatcgcctgg tgaaggaggg
     2041 gttcttccag cagctcttcg ggccgggcgt gagagccgag gagcagagca aggcaaagct
     2101 cgcgttcagg taacgcgaga ctactccaca gctcgcttgt gttggagggc ttcggccagc
     2161 ggcgtgttga caaaacggcc ggtcttagtc aagacgagcc ttggcgaatt caccgacaca
     2221 agcgagttgc ggggttagtc cggatgcaca agctcgagat cgatggcctg acgaagcggt
     2281 actacgtgga gcgagacggc cgccaggtgc tggctctctc cgccgtgtcg ctgtcggtgg
     2341 cggaaggcga gttcgtcgcc atcgtggggc cgagcggctg cggcaagacc acgctgctca
     2401 acatcgtcgc cggcctgctc ccgtacgacc tcgggaccgt ggcgatcgac ggcaggcgcg
     2461 tcgagggccc cggcgtcgat cgcgccgttg tgttccagca ctcgagcctg ctgccctggc
     2521 gaaccatcgc gggcaacgtg cgctacggga tggagctcca tcggcgcgtc gatcgcgcca
     2581 ccatgcagca gcgcactgat tacttcatcg ggctcgtcgg gctcacgggc ttcgagcgcc
     2641 actatccgtc ggagctgtcc ggcggcatgc agcagcgcgt caacctggcg cgggcgctcg
     2701 cctcggatcc ggtggtgctg ctcatggacg agccgtttgc ggcgctcgac gcgcagaccc
     2761 gcgagcagat gcaggtcgag ctgctgaaga tctggaccca cgcgcggaag accgtgctgt
     2821 tcatcacgca ccagatcgac gaagccgttt acctggccga ccgcgtggcg gtgatgagcg
     2881 cgaggccggg ccagatcaag ggggtgttcg acgtcccgtt cccccgcccg aggccgctga
     2941 gcgtgaaacg cgatcccggc ttcgccgcgc tcgaggacgc gatctggggt ctggtggacg
     3001 cccggcagga gcgcggcgat tcggtgggca ggcccgcatg agcgggacca gggagccgga
     3061 gctgatcgaa gcgccgcggc gcagtttcta tcagcggcac gagaggctga tcctcggcgg
     3121 cctctccgtc gtcgtcgtgc tcgccgtgtg gcaggcgttc tggtcggcgg gcaggatctc
     3181 gccgctgttc ttcaccgggc cgtcggccgt ggtgaagcgc ttcgccgagg aatggaccgg
     3241 gggccggctc aaggcggacc tcgtgtacag cgggaccaat ttcgtgatcg gtgtcggcct
     3301 ggcgattgcc accggcgtgg tgtgcggcgt cctgatcgga tggtaccggc gcctcggcat
     3361 ggtgttcgag ccgttcgtgg ccgcgctgta ctccacgccg cgcgtggcga tgatcccgct
     3421 tatcctgatc tggttcggta tcggcatgtg gtcgaaggtc ttcatcgtgt tcatcaacgc
     3481 cttcttcccc gtgctgatca acacgatcgg cggcgtcagg gcgatagact cggatctgct
     3541 ccgcgcggcg cgcgccttct gcgcctccga ctggcagatc ttcacgaccg tcgtgatacc
     3601 cggctcggtg ccgttcatcc tgaccggcgt gcgccaggcc gtggcgctgg ggctgatcgg
     3661 cgtcgtcgtg ggggagatgt tcggcggcag cgagggcatc ggctacatga cgaactacag
     3721 cggccagacc ttccagaccg acgccgtgtt cctgggcgtc gtgctgattg cgtgcgcggg
     3781 catcgtgctg acgtggctgg ccgaacggct cgagcggcac ttcagccgat ggagaccgga
     3841 aagataggca accgacgccg tagagcgcac gcttcagcgc gcgcggagac tgacgcatgg
     3901 cagagatcgt tcttggtatt ggatcgtcgc acgggccgct gctctcaacg ccgcccgagc
     3961 agtgggactt gcgggcgaag gccgaccgcg agaacaaggg gcactggttc cgggggaaga
     4021 cctacgattt caacacgctg ctcgcggcgc gggcgcccgg gttcgcccac gagatcaccg
     4081 tcgagaagcg tcgcgagcgg ttcttcaagt gccggcaggc gctcgaggcg ctcggcgtga
     4141 agttcaacca ggtctcgccc gacgccgtcg tgattctcgg caacgatcag cgggagttct
     4201 tcaacgccgg gttgacgccg tcgatcaccg tgtaccgcgg cagccaaatc agcaacgtgc
     4261 agcacctcaa cgaagacgcg ccgggcctca acatcgcgga gccggccaac tcaccgtcgg
     4321 aaggcgccac ctatccaggc gccactgccc tggccgatca tatcctcgac tcgctcgcgg
     4381 acgagaactt cgatctcgcg caatcggacc agacgccgag cggggcgcct cgcggcggca
     4441 ttccgcacgc ctacgtgttc ttctaccaca cgat
//
LOCUS       DBNGSH010000189         4913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3071616, whole genome shotgun sequence.
ACCESSION   DBNGSH010000189 DBNGSH010000000
VERSION     DBNGSH010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3071616"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="AC16CY_04285"
     CDS             complement(<1..755)
                     /locus_tag="AC16CY_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIR3353029.1"
                     /translation="MRYASSSYYRLALILGSLSATGPLAIDMYLPALPTIGHEFQTDV
                     GTVQVSLAVYFAGIAVGQAFYGPMSDRVGRKKVLYLGLSVFVVSSIGCALAGGVGSLI
                     GFRFLQALGGCAPLVVPRAIVRDHFDQAGSVRMLSVLMLVMGLAPILAPLIGGQLLVN
                     FGWRAVFWVLAAYATVCLVVVGFFLGESLPPDRRLAHPVGAVLAVYARLLRDRVFIGY
                     ALSGALVFAGLLSYISGSPAVFIEQFHVPPDRYG"
     gene            complement(785..1312)
                     /gene="moaC"
                     /locus_tag="AC16CY_04290"
     CDS             complement(785..1312)
                     /gene="moaC"
                     /locus_tag="AC16CY_04290"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR3353030.1"
                     /translation="MAQSRPRRPALSHVDEKGRARMVDVGGKPVTAREALARGAITMS
                     GPALRLVRGGGLAKGDPLEVARLAGIMAAKRTAGLIPLCHPLPLTHVSVELTPLRRGY
                     RIEARVRTSSQTGVEMEALTAVAVAALTIYDMVKAVDKEMVIGDICLVEKKGGRSGHY
                     VRNSKLKTQNSKLRT"
     gene            complement(1314..1973)
                     /locus_tag="AC16CY_04295"
     CDS             complement(1314..1973)
                     /locus_tag="AC16CY_04295"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR3353031.1"
                     /translation="MNRPNKRRQTSEQVSELTTNRLSVYLRCLNELDATGVQAISSQA
                     LAEQYQLNAAQIRKDLAYFGEFGVRGVGYYVKDLRRHLRQILGLDRRLRVAIMGAGNL
                     GLALADYPGFRQEGFEIAALFDAADEKIGHESRSGVPIYDIKDLKRIVRRERLDIAVI
                     AVPAPHAQPVVEQVVLAGIKAILNFSPGALKVPTDVKLKSVDLTVSLESLSFYLAQGD
                     Q"
     gene            2009..3958
                     /locus_tag="AC16CY_04300"
     CDS             2009..3958
                     /locus_tag="AC16CY_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIR3353032.1"
                     /translation="MKSAPAESKAHTSFQPYVPASKSQAEFTIKAIVLGVLFGLVFGA
                     STVYLGLRAGLTVSASIPIAVLAISVLKRLGGSTILENNIVQTIGSAGESLAAGVVFT
                     LPALIFLVPRGPEYFNYFQITVLAIAGGILGVLMMVPLRRALIVKEHGVLPYPEGTAC
                     ADVLVAGERGGALAKTVFLGLGIGALWKALSWIVQIFRTAIGYTFPRTSFFPNATLNI
                     DLSPEYLGVGYVIGPRIAGVMFSGGVLSWLVLLPLLSYLGNYMTVPLPPVPASGLRID
                     QMSAGQLWSAYIRYTGAGAVLAAGLITLARTLPTIVTSFRESVKDFGAAASGSARLRT
                     ERDMPLSVVVVGSLLLAVFLIVVPGAPTQGNVLSAVLVILFGFFFVTVSSRITGLIGN
                     SSNPISGMTIATLILTCTIFVALGMVGNDYAPIALGVGAVVCIAAAIAGATSQDLKTG
                     YIVGATPVSQQIGLVVGVVTSAFVIGITTLYLHSEMTIGSGSLPAPQATLMSTIIRGL
                     LSQDLPWGLVLVGVFISVTLELCGIRSLSFAVGSYLPIATTAPIFAGGVVRWWVERKT
                     GQRDESEISSGTLLSSGLIAGGSLAGILYAALYGNGIVAAADDAETLGLFPLLHEGTI
                     GMVAGGLLFAGLAVIVSRVAQRKVE"
     gene            3963..>4913
                     /locus_tag="AC16CY_04305"
     CDS             3963..>4913
                     /locus_tag="AC16CY_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353033.1"
                     /translation="MTRRASRARLIAALAGAALIVPPASPGLAAQAAGTPTRRLTTID
                     AVHQFPGFFHLQNVLLRGEFVTEGSRIVLRADEHEILVLFDGGVRAPSGVVDTRGLVV
                     DVGRLEPGDPRVGSFAEGRDAEKWPRPGEALLLRVNAVYERPSVAPVSVRSIAIEPWR
                     YGGQTVTVIGNFRGRNLFADLPGAPGKSPYDFVLRGAEGAIWVTDLRPRGAGFDLDVN
                     RRVDTNQWLEITGTVVHERGLVSLKATRLALAKQPKAEDPIDAGAPPPVIPSAPVVVV
                     FNSPSELETDVSPSARIRVQFSRGLNEASLEGNIRVSYAGQ"
BASE COUNT          794 a         1672 c         1615 g          832 t
ORIGIN      
        1 ccgtagcgat cgggaggcac gtgaaactgc tcgatgaaca ccgccgggga tcccgaaatg
       61 tacgagagca gcccggcaaa cacgagcgca cccgacaacg cataaccgat gaagacgcgg
      121 tcgcgcagga ggcgcgcgta gaccgcgagg acggcgccga cgggatgggc cagccggcga
      181 tcggggggca ggctctcacc caggaagaac ccgaccacga ccaggcagac cgtggcatac
      241 gcggcgagca cccagaacac ggcccgccaa ccgaagttga cgagcagctg ccctccgatg
      301 agcggcgcga gaatcggcgc caggcccatc acgagcatca gcaccgacag catgcgcacc
      361 gagccagcct ggtcgaagtg atcccggaca atggcccgag gcaccacgag cggcgcgcag
      421 cccccgagcg cttgaagaaa ccggaagccg atcagagacc cgacgccgcc cgccagagcg
      481 cagccgatcg aggacacgac gaacacggag agccccaggt agaggacctt cttgcgcccg
      541 acacggtccg acatcggtcc gtagaacgcc tgcccgacgg cgatccccgc gaagtacacc
      601 gccaggctca cctgcaccgt gccgacgtcg gtctggaact catgcccgat cgtcggaaga
      661 gccggcaggt acatgtcgat ggccagcggg cccgtcgccg acagcgaacc gagaatcagg
      721 gcgaggcggt agtaggagga ggaggcgtaa cgcaaaactc aaaactcgaa aggcaaaaag
      781 gagctcacgt tcgtagtttt gagttttgag ttttgagttt tgagttccgg acgtaatgcc
      841 ccgatcgtcc gcccttcttc tcgacaaggc agatgtcgcc gatcaccatc tccttgtcga
      901 ccgccttgac catgtcgtag atcgtcagcg ccgcgactgc gacggccgtc agcgcctcca
      961 tttccacacc ggtctggctc gatgtgcgga cgcgcgcctc gattcgatag ccgcgccgta
     1021 gcggcgtcag ctcgacgctg acgtgagtca gcggcagcgg gtgacacaac gggatcagtc
     1081 cggccgtgcg cttggcggcc atgatgcccg cgagccgcgc cacttcgagg gggtcgccct
     1141 tggccaggcc gccgcctctc acgagccgga gcgccgggcc gctcatcgtg atcgccccgc
     1201 gcgccagcgc ctcgcgcgcg gtcacgggct tgccgcccac gtcgaccatg cgcgccctcc
     1261 ccttctcgtc gacgtgcgag agcgcggggc gcctcgggcg ggactgcgcc aagctactgg
     1321 tccccctggg caaggtagaa cgacaggctc tcgagcgaca ccgtgaggtc aacgctcttg
     1381 agcttcacgt cggtcgggac tttgagcgct ccgggagaaa agttgaggat cgccttgatg
     1441 cccgcgagca cgacctgctc gacgaccggc tgcgcgtgag gagccgggac ggcgatgacg
     1501 gcgatgtcga gacgctcgcg ccggacgatg cgcttgaggt ccttgatgtc gtagatcggc
     1561 acgccgctgc gcgactcgtg gccgatcttt tcgtcggcgg cgtcgaagag cgccgcgatc
     1621 tcgaatcctt cctgcctgaa gcctgggtaa tcggccaggg cgagaccgag attgccggcc
     1681 cccatgatcg cgacacggag acgacggtcg aggccgagga tctgccggag gtggcgccgc
     1741 aggtccttga cgtagtaccc gacgccgcgc acgccgaact cgccgaagta cgccaggtcc
     1801 ttgcgaatct gcgccgcgtt caactgatac tgctcggcca acgcctgcga cgagatcgcc
     1861 tgcacgccgg tggcgtcgag ctcgttgagg catcgcaggt agacggacag gcggttggtc
     1921 gtgagctccg agacctgttc cgacgtctgc cgccgcttgt tcggccgatt cacaaggttc
     1981 aatgctacca tctcgcgcca tggcgcacat gaagtccgct cccgcggaat caaaggcaca
     2041 cacgtcgttt caaccgtatg tgccggcctc gaagtcgcag gccgaattca ccatcaaggc
     2101 gatcgtgttg ggcgtgctgt tcggactcgt gttcggcgca tcaacggtgt acctggggct
     2161 gcgcgcgggg ctgaccgtca gcgcgtcgat cccaatcgcc gtcctcgcca tctcggtcct
     2221 gaagcggctg ggcggatcga cgatcctcga gaacaacatc gtccagacga tcggatccgc
     2281 gggcgagtcg ctcgcggccg gcgtggtgtt cacgctcccc gccctgatct tcctcgtgcc
     2341 gcgcggcccg gagtacttca actacttcca gataaccgtg ctggcgatcg ccggcgggat
     2401 cctcggcgtg ctgatgatgg tcccgcttcg gcgggcgctg atcgtgaagg agcacggtgt
     2461 cctgccctac cccgagggta cggcgtgcgc cgacgtcctc gtggcgggcg agcgcggagg
     2521 cgcgctggcc aagaccgtct tcctcgggct cggcatcggc gccctgtgga aggcgctgtc
     2581 ctggatcgtc cagatcttca ggacggccat cggctacacg tttccgcgca ccagcttctt
     2641 tccgaacgcg acgctcaaca tcgatctgtc gccggagtac ctgggtgtcg gctacgtgat
     2701 cggtccgcgc atcgccggcg tgatgttctc cggaggggtg ctttcgtggc tggtgctcct
     2761 gccgctgctg tcttatctcg gcaactacat gacggtgccg ctcccgccgg ttcccgccag
     2821 cggccttcgt atcgaccaga tgagcgccgg gcaactctgg agcgcctaca tccgctacac
     2881 gggcgcggga gccgtgctgg ccgcgggcct catcacgctg gctcgaaccc tgccgacgat
     2941 tgtcacctcg ttccgggaga gcgtgaagga cttcggggcc gccgcgagcg gctcggctcg
     3001 cctgcggacc gagcgtgaca tgccgctcag cgtcgtggtc gtcggctcgc tgctgctggc
     3061 cgtctttctt atcgtcgtcc ccggggcccc gacacaaggc aacgtgctgt cggccgtcct
     3121 ggtcatcctg ttcggatttt tcttcgtcac cgtgtcgtcg cgcatcacgg gcctcatcgg
     3181 caactcgtcc aacccgattt cgggcatgac gatcgcaacc ctcatcctga cgtgcacgat
     3241 cttcgtggcc ctgggcatgg tcggcaacga ttacgcgccg atcgcgctcg gcgtcggcgc
     3301 cgtggtctgc atcgccgcgg cgatcgccgg agcgacgtcc caggacctga agaccggcta
     3361 catcgtcggc gcgacaccgg tctctcagca gatcggcctt gtggtcggcg tcgtgacgtc
     3421 ggcgttcgtg atcggtatca cgacgctcta cctccacagc gagatgacga tcgggtcggg
     3481 ctcgcttcca gcgccccagg cgactctgat gtccacgatc atccgcggcc tgctcagcca
     3541 ggacctgccg tggggtctcg tcctcgtcgg cgtgttcatc tcggtgacgc tcgagctctg
     3601 cggcatccga tcgctgtcgt tcgccgtcgg ctcgtacctc cccattgcga cgacagcccc
     3661 gattttcgcc ggcggcgtcg ttcgctggtg ggtcgagcgc aagacgggcc agcgcgacga
     3721 gtccgagatc agcagcggta cactcctgag ctccggcctg atcgcgggcg gttcgctggc
     3781 cggcatcctc tacgcggcgc tctacggcaa cggcatcgtc gccgccgccg acgacgccga
     3841 gacgctcggc ttgttcccgc ttctgcacga gggcacgatc gggatggtgg cgggcggcct
     3901 tctcttcgcg ggcctcgcgg tcatcgtctc cagggttgcg cagcgcaaag tagaatgaag
     3961 gagtgacccg ccgcgcttct cgagcccggc tgattgcggc gctggctggc gcggctctga
     4021 tcgtaccgcc ggcttcgccg ggtctcgccg cccaggcagc cgggacgcca acgcgtcgcc
     4081 tcacgaccat cgacgcggtg caccagttcc ccgggttttt ccacctgcag aacgtcctgc
     4141 tgcggggaga gttcgtcacc gaaggctcgc gcatcgtctt gagagccgac gaacacgaga
     4201 tcctcgtgct cttcgacggc ggcgtccgcg cgccgagcgg cgtcgtggac acgcgcggtc
     4261 tcgtcgtgga cgtgggccgc ctcgagccgg gcgatccgcg cgtcgggagc ttcgccgaag
     4321 ggcgcgacgc cgagaaatgg ccccggcctg gcgaagcgct gctgctccgc gtgaacgcgg
     4381 tctacgagag gccttcggtt gctccggtat cggtgcgatc gattgcgatc gagccgtggc
     4441 gctacggcgg ccagacggtg accgtcatcg gcaacttcag gggccggaac ctcttcgcgg
     4501 atctgcctgg cgccccgggc aagagccctt acgacttcgt tctccgtggc gccgagggcg
     4561 ccatctgggt caccgatctt cgcccgcgcg gggcgggttt cgacctcgac gtcaaccgcc
     4621 gggtcgacac gaaccagtgg ctcgaaataa ccggaaccgt ggtgcacgag aggggcctgg
     4681 tcagcctgaa ggcgacgcgg ctcgcgcttg ccaagcagcc gaaggcagag gacccgatcg
     4741 acgcgggcgc ccctccgcct gtcatcccgt cggcccccgt cgtggtggtc ttcaactcgc
     4801 ccagcgagct cgaaaccgac gtcagccctt cagcgcgaat ccgcgttcag ttctcgcggg
     4861 gactgaacga agcttccctt gaagggaaca tccgcgtcag ctacgcgggc cag
//
LOCUS       DBNGSH010000190         3325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4599895, whole genome shotgun sequence.
ACCESSION   DBNGSH010000190 DBNGSH010000000
VERSION     DBNGSH010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4599895"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="AC16CY_04310"
     CDS             complement(<1..325)
                     /locus_tag="AC16CY_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3353034.1"
                     /translation="MSSTTKTTKNAKGTKTCVSFIFVAFVAFAIFVAFVVPSGAAATS
                     DIADAAMRGDRAAVQALIAKKADVNAPQSDGATALHWAVYRGNRELAAILMRAGANPK
                     AANREG"
     gene            complement(334..1683)
                     /locus_tag="AC16CY_04315"
     CDS             complement(334..1683)
                     /locus_tag="AC16CY_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3353035.1"
                     /translation="MFISKKHIPRRTILRGAGAALALPFLEAMVPARTALAQTAAKGK
                     PRFVGLFVPHGAGPGYWVPDKVGPLGAELPFIYKSLEPLKDQLVIFSGLHSRSAEPPP
                     GVTGADHWVAAAFMCAEKPKKTAGADVQAGTTIDQIIAKKIGADSLLPSLQMAVEDPG
                     ANSSNCGEGYSCVYTNTISWATPTSPLPMELNPQVVFERMFGDGATAEQRAARRKRDQ
                     SILDSLTSSLSRLRGSLGASDRARLDDYEENVRAIERRLQIAMKASVVVPENVKVPVG
                     VPQTFDEHIKLQFDLLALAFEADITRVGSLLFARDLTGRTYPASEAPTSGFHGVSHHG
                     EDPRRILDLAKINKYHVAMVAHLASNLASKQEGDGTLLDHSMILFGSNMGNSNQHVHH
                     DVPHILVGGANGRLKGGRHLAYPSKTVPTGNLLLSVLDKFDIHQESLGDSTGRLEDL"
     gene            complement(1704..>3325)
                     /locus_tag="AC16CY_04320"
     CDS             complement(1704..>3325)
                     /locus_tag="AC16CY_04320"
                     /inference="COORDINATES: protein motif:HMM:NF019259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3353036.1"
                     /translation="VHLFDWDKEIANTTGKGRWTPRIPITAGHHVIGVTFLATNDVPG
                     TELNKPFQRTMNTPGSIPGFIFYPHVGQVTLEGPYDAKGATDTSSRRRIFTCRPSTPR
                     EEETCARSIVTTLAKRAFRRPSTAADLKQLMPFYQAGRSEGGSFEEGIESALQRILAD
                     PEFIYRGEREPAGLTAGRSYRLTDLELASRLSFFLWSSIPDDQLIDVAAAGKLKDPAV
                     LEQQVKRMLADPKSADLISNFTGQWLSVRSLKTVEPVVNLFPDFDDNLRSAFQREVEL
                     FFESIVREDRSVLDLLTADYTFVNERLAKHYGIPNIYGSQFRRVTLPPQLDVRRGLLG
                     KGALLTVTSNPARTSPVARGKWVQATLLGVEPPQPPPGVEVNILRANDNTGNTKQPTM
                     RQVLEQHRISPTCSACHKIFEPVGLALENFDAVGAWRTHDEGLPVDTSGKLPDGTPLD
                     GGVAALRASLVRYSDQFTRVVAEKLLTYALGRGVEHDDMPTVRAIVRGAESSQYRFSS
                     LVAGIVKSEPFQMNMKPSAQPEAAVRQRAAR"
BASE COUNT          499 a         1124 c         1112 g          590 t
ORIGIN      
        1 caccctcccg gttggcggcc ttcgggttcg cgcccgcgcg catcagtatc gcggccagct
       61 cgcgattgcc gcggtagacg gcccagtgca gcgcggtggc gccgtcgctc tgcggggcgt
      121 tcacgtccgc cttcttcgcg atgagcgcct gcacggccgc ccggtcgccg cgcatcgcgg
      181 cgtccgcgat gtcgctcgtc gcggcagccc ccgatggaac cacgaaggcc acgaagatcg
      241 cgaaggccac gaacgctacg aagatgaaag acacacaagt cttcgtgccc ttcgcgttct
      301 tcgtggtctt cgtggttgaa gacatctcgg ttctcacaga tcctccaacc gcccggtgct
      361 gtcgccgagg ctctcctggt gaatgtcgaa cttgtcgagg acgctcagca gcaggttccc
      421 cgtcggcacc gtcttgctcg ggtacgcgag atggcgtccg cccttgagcc tgccgttcgc
      481 gccgcccacg aggatgtgcg gcacgtcgtg gtgcacgtgc tggttcgagt tccccatgtt
      541 gctgccgaac agaatcatcg agtgatcgag cagcgtcccg tcgccctcct gcttcgaggc
      601 gaggttgctg gcgaggtggg ccaccatggc gacgtggtac ttgttgatct tcgccagatc
      661 cagaatgcgc cgcgggtcct cgccatgatg cgagacgccg tggaagccgg aggtcggcgc
      721 ttcgctcgcc gggtaggtgc ggccggtgag atcgcgcgcg aagaggagcg atccgacgcg
      781 cgtgatgtcg gcctcgaacg ccagcgccag caggtcgaac tgcaacttga tgtgctcgtc
      841 gaaggtctgc gggacgccga caggcacctt gacgttttcc ggcacgacca ccgacgcctt
      901 catcgcgatc tgcaggcgac gctcgatggc gcggacgttc tcctcgtagt cgtcgagccg
      961 cgcgcgatcg gacgccccga ggctgccgcg cagccgcgag aggctggacg tcagcgagtc
     1021 gaggatgctc tggtcgcgct tgcgccgcgc ggcccgctgc tcggcggtcg cgccgtcgcc
     1081 aaacatgcgc tcgaacacca cctgcggatt cagctccatc ggcagcggcg atgtcggcgt
     1141 cgcccaggag atcgtgttcg tgtagacgca gctgtatccc tcgccgcagt tgctcgagtt
     1201 cgcgcccgga tcctcgaccg ccatctggag cgacggcaac aggctgtcgg cgccgatctt
     1261 cttcgcgatg atctggtcga tcgtcgtgcc cgcctgcacg tccgcgcccg ccgtcttctt
     1321 cggcttctcg gcgcacatga acgccgcggc gacccagtgg tcggcgcccg tcacgccggg
     1381 cggcggctcc gccgatcggg aatgcaggcc gctgaagatg acgagctgat ccttgagcgg
     1441 ttcgagcgac ttgtagatga acgggagctc cgccccgagc ggcccgacct tgtccggcac
     1501 ccagtagccg ggccccgcgc cgtgcggcac gaacagcccc acgaatcgcg gcttcccctt
     1561 cgcggcggtc tgcgcgagcg ccgttcgcgc cgggaccatc gcttcgagga acggcagcgc
     1621 cagcgccgcg ccggcacccc tcagaatcgt tcgtcgtgga atgtgcttct tcgaaataaa
     1681 catgatgatg gctgtatcga aggttaacgg gccgcccgct gtcggacggc ggcttccggc
     1741 tgtgcactag gcttcatgtt catctggaac ggctcgctct tcacgatacc ggccacgagc
     1801 gacgagaacc ggtactggct cgactcggcg cctcgcacga tcgcgcgcac cgtcggcatg
     1861 tcgtcgtgtt ccacgccgcg gccgagcgcg taggtgagga gcttttcggc aaccacgcga
     1921 gtgaactgat ccgagtagcg cacgagagac gcccggagcg ccgccacgcc gccgtcgagc
     1981 ggcgtcccgt ccgggagctt gccgctggta tcgaccggca ggccttcgtc atgcgtacgc
     2041 caggcgccca cggcgtcgaa gttctcgagc gcgagcccga ccggctcgaa gatcttgtgg
     2101 caggccgagc acgtcgggct gatgcgatgc tgctccagca cctgccgcat cgtcggctgc
     2161 ttcgtgttgc cggtgttgtc gttggcgcgc aggatgttga cctcgacgcc cggcggcggc
     2221 tgcggcggct ccacgccgag cagcgtcgcc tggacccact tccctcgcgc gacgggcgag
     2281 gtccgtgccg ggttggacgt caccgtcagc aacgcgcctt ttcccagcag gccgcggcgc
     2341 acgtcgagct gcggcggcag cgtcacccgc cggaactgcg agccgtagat gttcgggatc
     2401 ccgtagtgct tggcgagccg ctcgttgacg aaggtgtagt cggccgtcag cagatcgagg
     2461 acgctgcgat cctcgcggac gatgctctcg aagaacagct cgacctcccg ctggaacgcg
     2521 ctccgcaggt tgtcgtcgaa gtccgggaac agattgacga ccggctccac tgtcttgagc
     2581 gatcgcacgc tcagccactg ccccgtgaag ttcgagatga gatcggcgga cttcggatcg
     2641 gccagcatcc gcttgacctg ctgctcgagc acggccgggt ccttcaactt acccgccgcg
     2701 gcgacgtcga tcagctggtc gtcgggaatg ctgctccaca ggaagaacga gagccgcgat
     2761 gccaactcga gatcggtgag ccggtagctc cggccggctg tcagccctgc gggctcgcgc
     2821 tcgccccggt agatgaactc cgggtcggcg aggatccgct gcagcgctga ctcgatcccc
     2881 tcttcgaagc tcccaccctc gctgcgcccc gcctggtaga acggcatcaa ctgcttcagg
     2941 tcggcggcgg tggacgggcg gcggaatgcg cgcttggcga gcgtggtgac gatgctccgc
     3001 gcgcacgtct cttcttcacg tggcgtggag gggcgacacg tgaagatccg ccgccggctc
     3061 gacgtgtccg tcgcgccctt cgcgtcgtac gggccttcga gcgtcacctg gccgacgtgc
     3121 gggtagaaga tgaagccggg aatcgagccg ggcgtgttca tcgtgcgctg gaacggcttg
     3181 ttcaactcgg tgcccggcac gtcgttggtc gcaaggaacg tcacgccgat gacgtgatga
     3241 cctgccgtga tcggaatgcg gggcgtccag cgccccttac ccgtcgtatt ggcaatctcc
     3301 ttgtcccagt cgaagaggtg gacgc
//
LOCUS       DBNGSH010000191         4870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1713127, whole genome shotgun sequence.
ACCESSION   DBNGSH010000191 DBNGSH010000000
VERSION     DBNGSH010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1713127"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="AC16CY_04325"
     CDS             complement(<1..553)
                     /locus_tag="AC16CY_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3353037.1"
                     /translation="MALDDILVILLAGGAGERLYPLTKERAKPAVYFGGPYRIIDFTL
                     SNCLNSGLRRIFIATQYKSLSLNRHVRMGWSVVSEELGEFIEILPPQKRVGEHWYLGT
                     ADAVYQNLYSVERESPSHVIVLSGDHVYKMDYSKMLHFHLEKGGDATIATIEVPIGQG
                     HRFGMVSIDSNERVIGFDEKPAHP"
     gene            775..1893
                     /locus_tag="AC16CY_04330"
     CDS             775..1893
                     /locus_tag="AC16CY_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353038.1"
                     /translation="MYGRRVVIPTLAVVVVTAAAPLLAQRGNSTTQKTPPPSPQQQDF
                     QALGVAVDAALMSDLGITLPAAGAPPSAPITPKPLAFGAGDRTEGAVSVKWRSNHFLK
                     AGNGETYLAYTLAFDRSQLAKGAALYVRIVSAEQAEAFAAYTAAAAAPPQSGKAPPAR
                     PMFGWESVGSVEVPSSGEISRAAQLKPGQYVAFVAAKERSAAAPVGLLRHELVVPDYN
                     RAELLTSSVIVAQSVEALPPSTQTNQESNPYVIGPMRIIPSFDHRFAKSGELIVFFFI
                     YGAKDAATGKPDVVVDFRFHQRLAEGEKYFNRMEPQQINAQTLPAEFSAAAGHQLRSD
                     QGLRLASFPVGDYRLEIKVTDRPGGRELTQSVNFSVVE"
     gene            2022..3851
                     /locus_tag="AC16CY_04335"
     CDS             2022..3851
                     /locus_tag="AC16CY_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3353039.1"
                     /translation="MGAQLTTVPQIATTPAPSFGQATARPYVDLRGVVTDDLGSPLAG
                     AVVSAQGATSAFAVSDQEGRFVLKGLPSGPYLLRAHLQNYAQLRGSVVQVGSSSGTST
                     IALTRRPDASTPPSVLAASVGAADERPRSAEGEAPHGHDERAWRLRRLKRSVLKEAEA
                     TLVDLNDEDSLLEGSLSTVGRAVGDSARLASALFGDLSVSGQINLIASTTFDHAQELF
                     TLSATAPNNVAYVAIEAPGADGDWTMRGTLRQGDLASWILAGSYVRRRPAAHAYEAGV
                     SFGMQRYIVGNGDALAVMPDSSRRVGAMYAMDHWTLSPQVSIGYGAKYARYDYLSDRG
                     LVSPRATLTLRPRANDTLALRTTVLHREVAPGAEEFLPAGMGLWLPPERTFSQVAGAP
                     MVPERHDLIQVAVDREWGGHVVIGVRAFTERVEDQVVTLFGVAVGDTPAGPGHYQIAS
                     AGDFGARGWGVTVARNVNDHVRATVDYTQSQTEWRRVSPDAEALSAIAPSALRGDERV
                     HDLTASVESVVPSTWTRLLVVYKLNTAFSPVLAEGNPGPSVRFDVQVNQALPFLNFMS
                     AEWEMLVAVSNLFKEQLYENSVYDELLVVRPPKRVLGGVTVRF"
     gene            4016..>4870
                     /locus_tag="AC16CY_04340"
     CDS             4016..>4870
                     /locus_tag="AC16CY_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3353040.1"
                     /translation="MPAAWSRWSRPALPVVVVSALLCLGIANVTSRATWKEVEDGVLW
                     ASNPEGVAAVEIAKGTPADRVGLRRGDILLAIDDHPIQSPADVVDVLHSGSDRPLRYT
                     ILRLGTREVIDVRLAPVPNGPEALYFVLASVGIFTLLVGGAVRLRRPRDPSTLHFFWL
                     AVAFFGVFTFSFSGRLDRWDWVFYWGDAVSMLALPPIFLHFTRGFPERRQRWNLGPAA
                     RARLALVYLPAIVLGVVRIYDVAKNSAQLETQLDRLGFMYFALCLIGGLITLLRALSQ
                     VGTMTARRQ"
BASE COUNT          791 a         1543 c         1627 g          909 t
ORIGIN      
        1 tcgggtgcgc cggtttttcg tcgaacccga tgacgcgttc gttcgagtcg atgctgacca
       61 tgccgaacct gtggccctgg ccaatgggga cctcgatcgt cgcaatcgtc gcatcgcccc
      121 ctttctcgag atggaagtgc agcatcttcg agtagtccat cttgtacacg tggtccccgg
      181 acagcacgat aacgtgcgaa ggcgattcgc gctcgactga atacaggttc tgatagacgg
      241 cgtcggccgt tccaaggtac cagtgctcgc ccacccgttt ctgcggcggc aggatctcga
      301 tgaactctcc gagctcctcc gacacgacgc tccaccccat tcgtacgtga cggttgagcg
      361 agagggactt gtattgcgtc gcgatgaaga tccgtcgaag gccggagttg aggcagttgc
      421 tgagcgtgaa gtcgatgatc cggtacggcc ctccgaagta gacggcgggc ttggcccgtt
      481 ccttggtcaa cgggtacagg cgctcgccgg cgccgccggc gagcaatatg acgaggatgt
      541 catctagtgc catgacaggt gcccgtcctt caccgatggg gcgcgcactc aaggcgccgg
      601 gacccgcctc agaggggtgg cgatcttaac acgcatgcgg ttttttcgag gctttttgta
      661 tgccggcgtg tttgacttgc cctccacagg tgcctatgat caggcgattc ggactcttgg
      721 atccccgctc aggcgggtgc cgtgcccgct cgccgctgga ctagagaggc atacatgtac
      781 ggtcgtcgcg tcgtcatccc gactctcgcc gtagtcgtcg ttaccgccgc cgcaccgctg
      841 cttgcgcagc gcggcaacag cacgactcaa aagacgcctc cgccttcacc gcagcagcaa
      901 gatttccagg cgctgggcgt cgcggtagat gctgcgctca tgtcggacct gggaatcacg
      961 ctgcctgcag ccggtgcacc gccgagcgcc ccgataacgc ccaagccgct tgcttttggc
     1021 gcgggagatc ggaccgaagg cgccgtgtcc gtcaagtggc ggtcgaacca tttcctcaag
     1081 gcaggaaacg gggaaacgta cctggcttac acgctggcct tcgatcgatc gcaactggcc
     1141 aagggcgccg cgttgtatgt ccgcatcgtg agcgccgagc aagcggaggc gtttgccgcc
     1201 tatacggcgg cagccgcggc tccgccgcaa agcggcaagg cgcccccggc gaggccgatg
     1261 ttcgggtggg agagcgtggg ctccgtcgag gttcccagca gtggtgagat ttcgcgggct
     1321 gctcagctca agccgggtca gtacgtggcg tttgtcgcgg ccaaagagag atcggccgcg
     1381 gcaccggtcg ggctgctccg ccatgagctc gtcgtccccg actacaaccg cgccgagttg
     1441 ctcaccagct cggtcatcgt cgcgcagtcg gtggaggcgc ttcccccttc gacccagacc
     1501 aatcaggagt cgaatccgta cgtcatcggg ccgatgcgaa tcatcccgtc gttcgaccac
     1561 cggttcgcca agagcggcga gttgattgtg ttcttcttca tctacggagc gaaggacgcg
     1621 gccaccggaa agcccgatgt cgtcgtcgac ttcaggtttc atcagcggct tgccgagggc
     1681 gagaagtact tcaatcgtat ggaaccgcag cagatcaacg cacagacgct tcccgccgag
     1741 ttcagcgcgg ctgccgggca ccaactgcgg tcggaccagg gcctgagact ggcgagtttt
     1801 cccgtgggcg actaccggct cgagatcaag gtgaccgacc gacccggtgg gagggagctg
     1861 acacagagcg ttaacttcag cgttgtagag tagtacgagg tcgggtcgaa gggcgaccgg
     1921 ctggccgccg gcctggagag aggctgacaa tgatgcggat gcgaactgga tcctggtgct
     1981 tcatcggggc catcgtgtgc gcgggcgtta acgcaacccc tgtgggggcg cagctgacca
     2041 ccgttccgca gattgccacg accccggcgc cgtcgttcgg tcaggcgacg gcgcgcccgt
     2101 atgtcgacct gcgtggagtg gtgaccgacg atctgggatc tccactggcc ggcgccgtcg
     2161 tgtccgcgca gggcgccacg tctgcttttg ccgtgtccga ccaggaaggc cgcttcgtgc
     2221 ttaagggcct tccgtcaggg ccctacctcc tgcgggccca cctgcagaat tacgcgcagt
     2281 tacgggggag cgtcgtgcag gtgggatcga gcagcggaac ctccacgatc gcgctgacgc
     2341 gccgccccga cgcatcaacg ccgccgagtg tccttgcggc gtcggtcggc gctgccgacg
     2401 agcgcccgcg atccgcagaa ggcgaggcac cacacggtca cgacgaacgc gcgtggcggc
     2461 tgcgccgcct caagcggagc gtcctgaaag aggctgaagc gacgctcgtg gacttgaacg
     2521 acgaggattc attgctcgag ggctcgctct cgacggtggg acgcgccgtc ggagactcgg
     2581 cacgattggc ttccgcgttg ttcggcgatc tctccgtgag cggtcagatc aatctgattg
     2641 cgtcaaccac gttcgatcat gcgcaagagc tcttcactct cagcgcgacc gcgccgaaca
     2701 acgtcgcgta cgtggcaatc gaagctccgg gcgccgacgg cgactggacc atgcgcggca
     2761 cgctcagaca gggtgacctg gcctcctgga ttctcgcggg ctcgtacgtg cgccggcgcc
     2821 cggcggcgca tgcctacgag gccggcgtgt ccttcgggat gcagcggtac attgtcggaa
     2881 acggggacgc gctcgcagtc atgcccgaca gcagccgcag ggtcggcgcg atgtacgcga
     2941 tggaccactg gacgctgtcg ccacaggtca gcatcggcta cggagcgaag tacgcgcgat
     3001 acgactacct gtcggatcga ggcctggtca gcccgcgcgc gaccctgacg ctgcgtcccc
     3061 gcgcgaacga caccctcgcg ctgcggacca cggttcttca ccgtgaggtg gcgccgggcg
     3121 ccgaggagtt cctgccggca gggatgggtc tctggctgcc tccggagagg accttctccc
     3181 aggtggccgg agcgccgatg gtgcccgagc gtcacgattt gattcaagtc gccgtcgatc
     3241 gggaatgggg cggacacgtc gtcatcggcg tgcgcgcgtt caccgagcgc gtcgaagatc
     3301 aggttgtcac gctgtttggc gtcgccgtcg gagacacgcc ggcggggcca ggtcattatc
     3361 agatcgcgtc tgccggcgac ttcggggcac gcggctgggg cgtcacggtc gcccgaaacg
     3421 tgaacgatca cgttcgcgcg acggtggact acacgcagtc gcagaccgaa tggcgccggg
     3481 tctcgcccga tgcggaagcg ctgagcgcga tcgctccgtc cgcgctcagg ggcgacgagc
     3541 gcgtgcacga cctgacggca tcggtcgaga gcgtcgtccc gtccacgtgg acgcgcctcc
     3601 tcgtcgtcta caagctgaac acggcgttct cccccgtgct cgccgaaggc aatccaggtc
     3661 cgtcggtgcg tttcgacgtg caggtcaacc aggcgctgcc gttcctgaac ttcatgagcg
     3721 cggagtggga gatgctcgtc gcggtgagca acctgttcaa ggagcagctg tacgagaact
     3781 cggtctacga cgagttgctc gtcgttcgac caccgaagcg cgtgctcgga ggtgtgaccg
     3841 tcagattctg acaagccggt agctggtagc tggtggttgg tagcgaagtg ctaccggcaa
     3901 ccggctatac gctaccagcc gggtgaaacc ggggagcatc gctccccatt ttttgacgca
     3961 attttcgagt ccatttactt ggatgccacg ttctagtcat tggatttcat cgcgaatgcc
     4021 tgcggcatgg agccgttggt cacggccggc gttgcccgtc gtggtcgtgt cggcgctctt
     4081 gtgtctcggc atcgccaacg tcacgtcgcg cgcgacatgg aaggaagtcg aggacggagt
     4141 cctttgggca tcgaatcccg aaggcgtggc ggctgtggaa attgccaagg gaaccccggc
     4201 tgaccgcgta ggcctgcggc ggggcgacat cctgctcgcg atcgacgacc acccgatcca
     4261 gtcgccagcc gatgtggtcg atgtgctgca ctcggggagc gaccggccgc tccgctacac
     4321 gatcctgaga ttggggaccc gggaggtcat cgacgttcgg ctcgcaccgg ttccgaacgg
     4381 ccccgaggcg ctgtatttcg tgctggcctc ggtgggcatc ttcaccttgc tcgtcggcgg
     4441 cgccgtgcgg ctgcggcgcc cgcgcgatcc gtcgacgctg catttcttct ggctggccgt
     4501 cgccttcttc ggcgtgttca cgttttcctt cagcggccgc ctcgaccggt gggactgggt
     4561 gttctactgg ggcgacgcgg tgtcgatgct cgcgctgccg ccgatcttcc tgcatttcac
     4621 ccgtggattc ccggagcgcc ggcagcgatg gaacctcggg ccggcggcgc gcgcgcgcct
     4681 ggcactcgtg tacctgccgg cgatcgttct tggagtggtg cgcatctacg acgtggcgaa
     4741 gaactccgcc cagctggaaa cgcagctgga tcgtctcggg ttcatgtact tcgcgctgtg
     4801 cctcatcggc gggttgatta cgctgctgcg cgcgctgtcg caggtgggca cgatgaccgc
     4861 gcgccgtcaa
//
LOCUS       DBNGSH010000192         4857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1373118, whole genome shotgun sequence.
ACCESSION   DBNGSH010000192 DBNGSH010000000
VERSION     DBNGSH010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1373118"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..837)
                     /locus_tag="AC16CY_04345"
     CDS             complement(1..837)
                     /locus_tag="AC16CY_04345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3353041.1"
                     /translation="MPPKTASAALVGAILGVAALLAWQQLGPGTATRGGHEATPPPTG
                     AAPCPAFNQSPLATNYLENLHGIEIGASTQNSFRLKRSINVDFSDEQGGLWQDKNCEP
                     ATVNIVAFGDDLPFKDEALDYVLSSHVIEHFYDPVKALREWHRVIRPGGYIFIIAPHK
                     DRTFDKSREVTPVAELIDRSTGKLTIADYVRAIDLPKVEQSGKATFGPGYEIPAQLLI
                     RRGAQPEEGWARYEEDDHHHWSVWRTNDFVDLVKALRFNIVEIQDTDDKVGNGFTVVI
                     RK"
     gene            892..1404
                     /locus_tag="AC16CY_04350"
     CDS             892..1404
                     /locus_tag="AC16CY_04350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3353042.1"
                     /translation="MAVFAEFVTSEGAFTVRLFDKEAPNTVANFVGLAEGTKEWTDPR
                     TNRKVKQRYYDGIIFHRVIAGFMIQGGDPLGQGIGGPGYEFADEFHPSLRHVKAGILS
                     MANRGPNTNGGQFFITLGPTPHLDNRHSVFGEVDSGMDVVEKIGATKTGPRDRPVKDV
                     VIQTIKIERR"
     gene            complement(1465..2271)
                     /locus_tag="AC16CY_04355"
     CDS             complement(1465..2271)
                     /locus_tag="AC16CY_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353043.1"
                     /translation="MKRRYLATLVAAGFLTAVTPVDAQSPSRNEVLKRVADYVKRFVD
                     VFGNVVAEEEYVQHFRGESERRRLKSDFLLVAYPGNTGLVMTFRDVLEVNGKPVGGKD
                     ERMTRLFLQPFDDAVRRAREIQSDGSRQSLGKSPLADPLAVLGFLQEFYQKDFHFSLG
                     GRARSLGPDIRELSMVRVVIGPRLSQRPFRARAWVSEITGEVVKTELNFQRLTTTTFR
                     SDPRFQIHVPAEMNDENPLGRNGTVLGTVTYGNFRRFEVRTESAVAAPNP"
     gene            complement(2321..3034)
                     /locus_tag="AC16CY_04360"
     CDS             complement(2321..3034)
                     /locus_tag="AC16CY_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353044.1"
                     /translation="MKTGLSAWLSLVLLGAFSAPAHAQGAYVSASLIGDVVRNTHTEL
                     SGTSDGSRGGEAIGFALRVGTEVASVFGVEVEFARPSVIESEWTPEVIPLADPRLASV
                     TSGGTIVPDPGLVFPIFQYQLRTAQRYTTLSTMLWARHDLSDRVSLAYLGGVSFQRTT
                     QEIEFEFQPVATLPPGLTFPTIYPLATKTVTYRAGPVAGIEGRIWLTDQVHLVPGVRL
                     HGLDGGWLVRSTVGLGWSF"
     gene            3173..>4857
                     /locus_tag="AC16CY_04365"
     CDS             3173..>4857
                     /locus_tag="AC16CY_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3353045.1"
                     /translation="MKRFYVLFAIVLSMTAGLTLAAQSQQTPFVPVTDEMLWKPSPND
                     WLTWRRTLDSWGYSPLNEINRNNVSRLKMSWTRGIVSGRTVESTPLVYNGTMYIPNPG
                     DIIMAMDAKTGDLKWEYRRKYPGGVNGGTNRNMAIWGTTLIDAGADNAMYAVDARTGE
                     LVWETQVLKAGVRANAGSGPIIANGKIITGRQCQPDATSDSCIITAHDAQTGKEVWRT
                     RTIPRPGEPGDETWGGVPLEQRWHVGTWMVPSYDPETNLIFVGTSVTIPAPKFTLGGA
                     DKQHLYHNSTLALDADTGKIVWHYQHVIDNWDLDHPFERILVDTAIAPDAREVAWINP
                     RIKPGERRKVITGIPGKTGVVYTLDRRTGEFLWARPTVMQNVISKIDGATGAVTVNPE
                     AIFTAKDQTKLICPGSNGGKNWPAGAYSPATNTMYMPLQNMCMNAKTTTDQRDPKLVY
                     GLDMPGVIAPGATNVGTVYAVSAETGKTVWKHEQRAGMLSLVATGGGLVFGGDANGRF
                     KAFDDKTGKVLWEVNLGSPVSGFPVTFAVDGKQYVAVTTGPSLVAGSAGRMTQ"
BASE COUNT          931 a         1580 c         1548 g          798 t
ORIGIN      
        1 tcactttctg atgacgacgg tgaatccatt gccgaccttg tcgtcggtgt cctggatctc
       61 gacgatgttg aatcgcagcg ccttcacgag atcgacgaag tcgttcgttc gccagaccga
      121 ccaatgatga tgatcgtctt cctcgtaccg tgcccacccc tcctcgggct gtgcaccccg
      181 tcgtatcagc agctgagcgg ggatttcgta gccggggccg aacgtcgcct tgcccgactg
      241 ttccaccttc gggaggtcga tggccctcac gtaatccgca atcgtgagct tgcccgtcga
      301 ccggtcgatc aactccgcca ccggcgtgac ctcgcgggac ttgtcgaacg tcctgtcctt
      361 gtggggagcg atgatgaaga tatacccgcc tggcctgatc accctgtgcc actcccgtaa
      421 cgccttcacc gggtcgtaga aatgctcgat gacgtgagat gacagcacgt agtcgagcgc
      481 ctcgtccttg aacggcaggt cgtctccgaa ggcgacgatg ttcacggttg ccggctcgca
      541 gttcttgtcc tgccacaagc ctccctgctc gtcagagaaa tcgacgttga tggaccgctt
      601 caggcgaaag gagttctgcg tcgaggcgcc gatctcgatg ccgtgcaggt tctcgaggta
      661 gttggtggcg agcggcgact ggttgaatgc cgggcaaggc gccgcgccgg ttgggggcgg
      721 cgttgcctcg tgtccgccgc gcgtcgcggt tccgggtccg agctgctgcc acgcgagcag
      781 cgcagcgact cccagaatgg cgccgacgag ggcggcggac gcggtcttag gcggcatcgg
      841 acgatcgaaa aattgtatgc tcagtggcca ccatctaacc tgggagtcgc cgtggccgta
      901 tttgcagagt tcgtgacgag cgagggcgcg ttcaccgtcc gcctgttcga caaggaagcg
      961 ccgaacacgg tggccaactt cgtgggtctg gcggagggca ccaaggagtg gaccgacccc
     1021 cgcacgaacc ggaaggtgaa gcagcggtac tacgacggca tcatctttca ccgcgtgatc
     1081 gcgggattca tgatccaggg gggcgacccc ctcgggcagg gcatcggcgg tccgggctac
     1141 gagttcgcgg acgaattcca cccctcgctg cgccacgtca aggcgggaat cctgtcgatg
     1201 gccaatcgcg gtccgaacac caacgggggg cagttcttca tcacgctcgg cccgacgccc
     1261 cacctcgaca acaggcactc cgtgttcggt gaggtcgaca gcggcatgga cgtcgtcgag
     1321 aagatcggcg ccacgaagac cggcccgcgc gaccggccgg tgaaagacgt cgtcatccag
     1381 acgatcaaga tcgagaggcg ttaaatgggg tcgggagtca ttttccctgc ctacggaaaa
     1441 tgactcccga ccccattaac cgccctacgg attaggcgcg gcaacggccg actcggtgcg
     1501 aacctcaaac cgtcgaaaat tcccgtacgt caccgttccc agcacggtac cgttccgacc
     1561 gagcggattc tcgtcgttca tctcggccgg aacgtggatc tggaatcgcg gatccgatct
     1621 gaaggtcgtg gtcgtcagcc gttgaaagtt cagttcggtc ttcacgactt caccggtgat
     1681 ttccgagacc cacgcgcgtg cccgaaacgg ccgctgcgag agtctcggcc cgatcacgac
     1741 gcggaccata gacagctcgc ggatatcggg accgagcgat ctcgcccgcc cgccaagcga
     1801 gaaatgaaaa tccttctggt agaactcctg caggaagccc aggacggcca atggatcggc
     1861 cagagggctc ttcccgaggc tctggcgtga tccgtcgctc tgtatctcgc gcgcgcggcg
     1921 aacggcgtcg tcaaacggct gcagaaacag ccgcgtcatc cgttcgtcct tcccgccgac
     1981 aggtttcccg ttgacttcga gtacgtcgcg gaacgtcatc accaggcccg tgtttcctgg
     2041 atacgcgacg agcaggaaat cggatttcag gcggcgccgt tcgctctcac ctcgaaaatg
     2101 ctgaacgtac tcctcctctg ccacgacgtt gccgaagaca tcgacgaagc gcttcacgta
     2161 atcggcgaca cgtttgagca cctcattccg tgacggcgac tgagcatcga ccggcgtcac
     2221 cgctgtcagg aaacccgccg ccacgagagt cgcgagatat cgacgcttca tcgggctgca
     2281 gccaccaccc tgggagtcgg aggttgggag ttgggagttc tcagaaactc cagccgagac
     2341 cgacggtcga acgcaccagc catccaccgt cgaggccgtg cagccggacg ccgggcacga
     2401 ggtgcacctg atcggtcagc cagatgcgcc cttcgatgcc tgcgaccggg cccgcgcgat
     2461 aggtcacagt cttcgtcgcc aacggataga tcgtcggaaa cgtcagacct ggaggcagag
     2521 tcgcaaccgg ctggaactcg aactcgatct cctgcgtcgt gcgttggaaa ctcacgccgc
     2581 cgaggtaggc aagcgagacg cgatcggaca gatcatgccg cgcccagagc atggtggaga
     2641 gcgtcgtata gcgctgtgcg gtgcgaagct gatactggaa gatggggaac accagaccgg
     2701 ggtcgggcac gatcgttccc cccgaagtga cgctcgccag cctggggtcg gccagcggga
     2761 tcacctcagg cgtccactcg gactcgatga ccgacggccg cgcgaactcc acttcaacgc
     2821 caaagacgga cgcaacctcg gttcccacgc gaagcgcgaa cccgatcgct tccccgcccc
     2881 tggatccatc ggaggttcca gacagctccg tatgcgtgtt ccggacgacg tcgccgatca
     2941 gcgaggcgct gacgtaggcg ccctgcgcgt gggcgggcgc cgagaacgct ccgagcagca
     3001 ccagcgacag ccacgccgat aggcccgtct tcatggggcg atcttacaca atgccgggcc
     3061 gcagtcaggc caggctgcct cccgttcagc gggcacatgt tcgtcggtcg gcagccagct
     3121 gatcgcgtat gctttgggcg gccacgttca ctcttccgtg aggggtatcg acatgaagcg
     3181 cttctacgtc ctgtttgcca ttgttctcag catgaccgcg ggcttgacgc tcgccgccca
     3241 gagccagcag acgccattcg tcccggtgac cgacgagatg ctgtggaagc cgagccccaa
     3301 cgactggctg acgtggcggc ggaccctcga cagctggggc tacagcccgc tcaacgagat
     3361 caaccggaac aacgtctcgc ggctgaagat gtcgtggacg cgcggtatcg tctcgggccg
     3421 cacggtggag tccacgccgc tcgtctacaa cggcacgatg tacatcccga acccgggcga
     3481 catcatcatg gcgatggacg ccaagacggg cgacctgaag tgggagtacc gtcgcaagta
     3541 cccgggcggc gtcaacggcg gcaccaaccg caacatggcg atctggggga cgacgctcat
     3601 cgacgcgggc gcggacaacg cgatgtacgc cgtcgatgcg cgaacgggcg aactggtctg
     3661 ggagacgcag gtgctcaagg ccggggttcg cgccaacgcc ggctcgggac cgatcatcgc
     3721 caacggaaaa atcatcacgg gacgccagtg ccagcccgac gcgacgagcg actcctgcat
     3781 catcacggcg cacgacgcgc agaccggcaa ggaggtctgg cgcacccgca cgattccgcg
     3841 cccgggcgag cccggagacg agacctgggg cggcgtgccg ctcgaacagc gctggcacgt
     3901 cggcacgtgg atggtgccga gctacgaccc cgagacgaac ctgatcttcg tcggcacgtc
     3961 ggtgacgatt cccgcgccga agttcacgct cggcggcgct gacaagcagc acctctatca
     4021 caactcgacg ctcgccctcg acgctgacac cgggaagatc gtctggcact accagcacgt
     4081 gatcgacaac tgggatctcg accacccctt cgagcggatc ctggtggaca cggccatcgc
     4141 gcccgacgcg cgcgaggtcg cctggatcaa cccgaggatc aagcccggcg agcggcgcaa
     4201 ggtcatcacc ggcattcccg gcaagacggg cgtcgtctac acgctcgatc ggcgcacggg
     4261 cgagttcctg tgggcgcgcc ccacggtcat gcagaacgtg atcagcaaga tcgacggcgc
     4321 caccggcgcg gtgaccgtga atcccgaggc gatcttcacg gccaaggatc agacgaagct
     4381 gatttgtcct ggcagcaacg gagggaagaa ctggccggcc ggggcctaca gccccgcgac
     4441 gaacacgatg tacatgccgc tgcagaacat gtgcatgaac gccaagacga cgaccgatca
     4501 gcgggatccg aagctcgtgt acggcctcga catgccgggc gtcatcgcgc cgggcgcgac
     4561 caacgtcggc accgtgtatg ccgtctcggc cgaaaccggc aagacggtct ggaagcacga
     4621 gcagcgcgcg ggcatgctgt cgctcgtcgc cacgggtggt ggactcgtct tcggcggcga
     4681 cgccaacggg cggttcaagg ccttcgacga caagaccggc aaggtcctgt gggaagtgaa
     4741 cctcggctcg cccgtcagcg gctttccggt gacgttcgcc gtggacggca agcagtacgt
     4801 cgcggtcacg accgggccgt cgctcgtcgc cggctccgcg ggacgcatga cgcagga
//
LOCUS       DBNGSH010000193         2220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4090647, whole genome shotgun sequence.
ACCESSION   DBNGSH010000193 DBNGSH010000000
VERSION     DBNGSH010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4090647"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="AC16CY_04370"
     CDS             complement(<1..603)
                     /locus_tag="AC16CY_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353046.1"
                     /translation="MRRQLLSLLLVPAALLSLRVLDAQAPAGGAPAPQPNVVVPGELV
                     IEPPTLINLGFEWFIQGDDNRNASVAVSYRKAGTTDWRTALPLLRLQGERIYAESRVD
                     VVAPNMFAGSVLDLEPDTAYEVRFLISDPDGVGGPASRAVSVRTRAEPQAYAGGRVFH
                     VYPHGHTGAKVEPAFEGLMCAYNFWCAGTDWATSGRPRVRP"
     gene            complement(605..1507)
                     /locus_tag="AC16CY_04375"
     CDS             complement(605..1507)
                     /locus_tag="AC16CY_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353047.1"
                     /translation="MNVKSYLISLPERLVRSIVGLGAGVAREVGEVAIPGGVRRTRLY
                     QNLVDTTLRFLIEQVGGVEGVFRDDATLADDFLVRRTAGNAIEALGIVAFRASPVWVL
                     AALSDVCGIGRHVIPEIAAALKERGLLEHDAQFTSVDQLLDGLERTSGRMANAVNTPP
                     LDIAGLRQEWRAIREEARAIGPERLPSRESLTRLWTDLRTEAGRQERSVFETSSMMAV
                     SAVKGLPDGARWLGASAMVGATRTGQILAASLLDYYRTTLQDVREVGYATYAVRQLRP
                     YVRAAASHMAPERRTLTERFLERK"
     gene            1626..>2220
                     /locus_tag="AC16CY_04380"
     CDS             1626..>2220
                     /locus_tag="AC16CY_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HIR3353048.1"
                     /translation="MTSARERLLLIAAFLSVSSPYLTAQNPVSPLFSSPEVGSDRRIT
                     LRIAAPQAEAVRLSASDIPGGGQGAEMIKGQNGVWETTVGPVPAGAYRYRFTVDGVAT
                     MDPRNPSTSESNTNAWSLVYVDGSDTSDTKNVPHGSVSEVTYHSTALNAVRRMHVYTP
                     PGYETSTRKYPVFYLLHGAGDSDDSWSTVGRAGFILDN"
BASE COUNT          378 a          783 c          722 g          337 t
ORIGIN      
        1 cggccggacc cgaggtcggc ccgacgtcgc ccagtcggtg cccgcgcacc agaagttgta
       61 cgcgcacatc agtccttcga acgcgggctc gaccttcgcg cccgtgtggc cgtgcggata
      121 gacgtgaaag acacggccac cggcgtaggc ctggggttcc gctctcgtcc ggaccgacac
      181 cgctcggctc gcgggccccc cgacgccgtc cggatccgat atcaggaagc gcacctcgta
      241 ggccgtgtcg ggctccaggt ccaggacgct gcccgcaaac atgttgggcg caacgacgtc
      301 cacgcgcgac tcggcgtaga tgcgctcgcc ctgcagccgc agcaacggaa gcgccgtcct
      361 ccagtcggtt gtgccggcct tgcggtatga cacggcgacc gaggcgttgc ggttgtcgtc
      421 gccttggatg aaccactcga agccgaggtt gatgagcgtc ggcggctcga tgacgagttc
      481 gccgggaacg acgacgttcg gctgcggcgc cggcgctccc cctgccggtg cctgcgcatc
      541 gaggacgcgc agcgacagca gcgcagccgg cacgagcaga agcgaaagca gttgtcggcg
      601 catatcactt cctctcgagg aaacgttcgg tcagcgtgcg tcgctcgggc gccatgtgcg
      661 acgccgccgc gcggacgtac gggcgcagct gacgtaccgc gtaggtcgca tagccgacct
      721 cccgcacgtc ctgcagcgtg gtccgatagt aatcgagcag cgacgccgcg agaatctgcc
      781 cggtccgcgt ggccccgacc atggccgacg ccccgagcca gcgagcgccg tcgggcagcc
      841 ccttgaccgc cgacaccgcc atcatcgacg aggtttcgaa caccgaccgc tcctggcggc
      901 ccgcctcggt tcgaagatcg gtccaaagcc gggtcagcga ctcccgcgac ggcagccgtt
      961 ccggcccgat ggcgcgcgct tcctcgcgga tggcgcgcca ctcctgccgc aatccggcaa
     1021 tgtcgagagg cggcgtgttg acggcgttcg ccatccggcc ggacgtgcgc tcgaggccgt
     1081 ccagaagctg gtccacgctc gtgaactgtg cgtcgtgttc gagcagcccg cgctccttga
     1141 gcgccgcggc gatctcggga atcacgtgcc tcccgatgcc gcacacatcc gacagcgctg
     1201 cgagcaccca gacgggcgac gcccggaacg ccacgatgcc gagggcctcg atggcgttgc
     1261 ccgcagttcg cctgaccagg aaatcgtccg caagcgttgc gtcgtcacgg aacacgcctt
     1321 cgacgccgcc cacctgctcg atgagaaacc tcaacgtcgt gtccacgagg ttctgataga
     1381 gccgcgtccg gcggaccccg cccgggatcg cgacctcgcc tacctcgcgc gcgacgccgg
     1441 caccgaggcc gacgatggag cggacgagcc gttcgggcag ggagatgaga tacgacttga
     1501 cgttcatggc gaccgggaac tttcgcgata ataaccggga ctccgcctat cgccgcggca
     1561 cgacgcaagg ctgagcgctc gtgagccgct acccttcgac aaggcctcgt cctacaatgc
     1621 acgctatgac cagcgcacgt gaacgtctgc tgctgattgc cgctttcctg tcggtctcct
     1681 cgccgtacct cacggcccag aacccggtgt cgcccctgtt ctcctcgccc gaggtcggct
     1741 cggatcgtcg catcacgctc aggattgcgg cgccacaggc ggaagccgtc cggctgtcgg
     1801 ccagcgacat tcccgggggc ggccagggtg cggagatgat caaggggcag aacggtgtgt
     1861 gggagacgac ggtcgggcct gtcccagctg gagcttacag gtacaggttc acggtcgacg
     1921 gggtcgccac gatggacccc cgcaacccgt caacgagcga atcgaacacg aacgcgtgga
     1981 gcctcgtcta cgtcgacgga tcggacacgt cggatacgaa gaacgtcccc cacgggagcg
     2041 tctccgaagt gacgtaccac tccacggcgc tcaacgcggt tcggcggatg cacgtgtata
     2101 caccgcccgg ctacgaaacc agcacccgca agtatccggt cttctatctc ctgcacggcg
     2161 cgggcgacag cgacgactcc tggagcacgg tcggccgcgc gggattcatt ctggacaacc
//
LOCUS       DBNGSH010000194         4468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_184703, whole genome shotgun sequence.
ACCESSION   DBNGSH010000194 DBNGSH010000000
VERSION     DBNGSH010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_184703"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..511)
                     /locus_tag="AC16CY_04385"
     CDS             complement(176..511)
                     /locus_tag="AC16CY_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353049.1"
                     /translation="MKKRTTKTTDPLDGPVDFSRFGPVRRDAFAGAGETGGPSLQALW
                     EMPQLATDAVLLRRGRPGKGHRRTSTVRSVRLPEALWKEMERAARGKRLNLHQAMRAA
                     LLGWIRRAG"
     gene            complement(570..1481)
                     /locus_tag="AC16CY_04390"
     CDS             complement(570..1481)
                     /locus_tag="AC16CY_04390"
                     /inference="COORDINATES: protein motif:HMM:NF052887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8021 domain-containing protein"
                     /protein_id="HIR3353050.1"
                     /translation="MKRLALMCIVLTGALLAPTTGFAQISCSRDGLRAAADLYIAAQT
                     KGDVSGLPLATGLGYMENMARADIETGLIKTAMTIDHHRSLIDTSTCQTFTEVIVTNK
                     EKPYVLGTRLRVNRDKIAEIEILWTTTGYWLFNADAYLKWSSSENWGIIPANQRDTRD
                     TLVAAANAYLDAFLEGRKDLVPWGYPCNRTEGGLHTGKGVPEDSCDVGVPSGVNIANR
                     RFIVDETIGSVVAFCTFGAGSAAGGSGAPDTHLFRVEGGKLRYVHTLTHLLQSNFGGR
                     GGGRGAAGGRGAAGGGQGGQAPPNTQK"
     gene            complement(1554..3662)
                     /locus_tag="AC16CY_04395"
     CDS             complement(1554..3662)
                     /locus_tag="AC16CY_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019156.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3353051.1"
                     /translation="MREWWRSLCTPRAEPVPREIDEVRTLRDGFERLDDESLKRAARS
                     ATRVPEIVAVTAVVASRVLGLHMFDPQLEGALALTHGRIVEMQTGEGKTLAAVPAVAW
                     YARAGGGVHVLTANDYLARRDAEWMGKTYHWLGLSVAAVTQEMTAEERRAAYRRDITY
                     ASANELGFDYLRDGLALHPGEQVLRPFAAAVIDEADSILLDEARIPLVIAGGTMEPSA
                     AAVDADRVVRHLVPHAHFTSDQIARNVALTPQGVAEVERLLPCGNLYDPGHLSVMTAV
                     QDALHAHRLLHRDVDYLVKDSMVLSIDELKGRVVRDRRWPAGLQTALELKEQVTRHRQ
                     GRVLGSITMENLIGLYPAVCGMTGTAAAQETEFRELYGLEVAVIAPNRPVIRNDDSDR
                     GFATRAEKEDAVLDEIRRRCQSRQPVLVGTSSVEESERLSALLRDVPHQVLNARNEET
                     EAAIVARAGEAGAVTISTNMAGRGVDIRLGAGVAELGGLHVIGTNRHESSRIDRQLRG
                     RAGRQGDPGSSQFFVSLQDPLFMKYADGPGTPLSSCDHLQRVAEGQSLGCRLFLRKYE
                     TVVEGQRQRIADRRQQVLTGPSASELDRLVTLDTIDELWSDYLAVVSELRASTIWVSL
                     GGGNPFREYLRTVHEMFQELTRTIDEEITVRLQRAATHGIEPRQRGATWTYLTTDEPF
                     GTMTERLMRRLAVMLHLLRS"
     gene            3870..4067
                     /locus_tag="AC16CY_04400"
     CDS             3870..4067
                     /locus_tag="AC16CY_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353052.1"
                     /translation="MFGLLRRLQPFDGNDEAQRLERPRRSRRRACEGEHDDGQGGQAE
                     AAGHEGQYTATEETIAKLKDK"
     gene            4128..>4468
                     /locus_tag="AC16CY_04405"
     CDS             4128..>4468
                     /locus_tag="AC16CY_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353053.1"
                     /translation="MRFASIKYVFLLALLVLTSLSNPSAQQASQTGQGPSLASIGPIV
                     FGPDGTLFAADSQGASIFALDLGAQANAGAANAKGLDALDEKLGALLGTAAKEITITD
                     LAVHPRTRNAYV"
BASE COUNT          734 a         1530 c         1458 g          746 t
ORIGIN      
        1 ggagatcctt ccgaaatcgg acgcgctctc ggcgcgttac gggcagagcg acggccggct
       61 gtttctcgtg cgcccggatg gctacatcgc cttcaagagc tgcgcggacg aggccggcct
      121 tctcgaggcc cacatggcga aaatcctgac gctctagtgg ccgcgcgatg cggcgtcacc
      181 ctgcccggcg aatccaccca agcagtgcgg cgcgcatcgc ctggtgcaga ttcagccgct
      241 tcccgcgtgc cgcccgttcc atttccttcc agagtgcttc gggcagccgg accgatcgca
      301 cagtcgacgt tcgacgatgc cccttccccg gacgaccacg ccgcagcaac accgcgtcgg
      361 tggccagctg cggcatttcc cacagcgcct ggagcgacgg tccgcctgtc tcgccggctc
      421 cggcgaacgc gtcgcgacgg acgggtccga accgtgagaa atccaccgga ccgtccagtg
      481 gatcagtcgt tttggtagtg cgctttttca tggcgcgatc tcatccgccc catcagtgct
      541 cggcgggcaa ccagccggcc cgaggcgcgc tacttctgcg tgttcggagg cgcctggccg
      601 ccctgaccac caccagctgc gccccggccg cccgcagcgc cgcggccacc gccacggccg
      661 ccgaagttcg actgcagcag gtgcgtcagc gtatgcacgt agcgcagctt gccgccctcg
      721 acgcggaaga gatgcgtatc gggtgcgccg ctgccgccgg cggcgcttcc ggcgccgaag
      781 gtgcagaacg cgaccaccga gccgatggtc tcgtccacga tgaaccggcg gttggcgatg
      841 ttgacgccgc tcgggacgcc gacgtcacaa ctgtcttcgg gaaccccctt gcccgtgtgc
      901 aagccgcctt cggtgcggtt gcaggggtag ccccacggca ccaggtcctt cctcccctcg
      961 aggaacgcgt cgaggtaggc gttggcagcg gccaccagcg tgtcgcgcgt atcgcgctgg
     1021 ttggcgggaa tgattcccca gttctctgag gacgaccact tcaggtaggc gtcggcgttg
     1081 aacagccagt agccagtcgt ggtccagagg atttcgatct cggcgatctt gtcgcggttg
     1141 acgcgcagcc gcgtgccgag gacgtacggc ttctccttgt tcgtcacgat cacttcggta
     1201 aaggtctggc acgtcgacgt gtcgatgagg ctgcgatggt ggtcgatcgt catcgccgtc
     1261 ttgatcaagc ccgtctcgat gtcggcgcgc gccatgttct ccatgtagcc gagtcccgtg
     1321 gcgagcggca ggccggagac atcgcccttg gtctgcgcgg cgatatagag gtcggctgcg
     1381 gcccgcaacc cgtcgcgcga gcaggaaatc tgggcgaagc ccgtcgtcgg ggcgagcagc
     1441 gcgccggtga gcactatgca catgagagca aggcgtttca tccaactcct ccagcaggcc
     1501 attatgaata tcatctttgc cgtgatctgg cgaagcgcgt cgcggcctcc cgctcaggac
     1561 cgcagcaggt ggagcatgac ggccagcctc cgcatcaagc gctcggtcat ggttccgaac
     1621 ggctcgtcgg tcgtcagata cgtccaggtc gcgccccgct gtcgtggctc gatcccgtga
     1681 gtggccgcac gctggagacg gaccgtgatc tcttcgtcga tcgtccgcgt gagctcctgg
     1741 aacatctcgt gtaccgtgcg cagatactcg cgaaacgggt tgccgccgcc gagcgacacc
     1801 cagatggtgc tcgcgcgaag ctcgctgacg acggccaggt aatccgacca gagctcgtcg
     1861 atcgtgtcga gcgtgaccag gcgatcgagc tccgaggctg agggccccgt cagcacctgc
     1921 tggcggcgat cggcaatccg ctgccgctgt ccttcgacga ccgtctcgta cttgcggaga
     1981 aagaggcggc agccaaggct ctggccctcg gccacgcgct gaagatgatc gcaggaagac
     2041 aacggtgtcc cggggccgtc cgcgtatttc atgaagagcg gatcctggag cgacacgaag
     2101 aactgcgagc tgccgggatc gccctgccgg ccggcgcgtc ctcgcagctg tcgatcgatc
     2161 cggctgctct cgtgccggtt ggtgccaatc acgtgcaggc cgccgagctc ggcgacgccg
     2221 gcgccgagac ggatgtcgac gccgcggccc gccatgttcg tcgagatggt gaccgcgccc
     2281 gcctcgccgg cgcgcgccac gatggcggcc tcggtctctt cattgcgcgc gttgagcacc
     2341 tggtgaggca cgtcgcgcag gagcgcgctc agccgctccg attcctcgac cgatgacgta
     2401 ccgacgagaa cgggctgcct cgactgacac cggcggcgaa tctcgtcgag gaccgcgtcc
     2461 tccttctcgg cgcgagtcgc gaacccgcga tccgaatcat cgtttctgat cacgggacga
     2521 ttcggcgcga tcactgcgac ctcgagacca tagagctccc ggaactcggt ctcttgcgcg
     2581 gccgccgttc ccgtcatccc gcacacggcg ggatagagcc cgatcaggtt ctccatcgtg
     2641 atcgagccga gcacgcggcc ctgccggtgc cgcgtcacct gctctttcag ctcgagcgcc
     2701 gtctgcagtc ccgccggcca gcgacggtcg cgcacgacgc gccccttgag ctcgtcgatg
     2761 gacaggacca tcgagtcctt caccagatag tccacgtcgc ggtggagcag cctgtgcgca
     2821 tgcagcgcgt cctgaacggc ggtcatgaca gagagatgtc cggggtcgta gagattgccg
     2881 cagggcagga gccgttcgac ttcggcgacg ccctggggtg tgagcgcgac gttcctcgcg
     2941 atctgatcgg acgtgaagtg cgcgtgcggg accagatgac gaacgacccg atcggcgtcc
     3001 accgcagcgg cagagggctc catcgtgccg ccggcgatga cgagcggaat cctcgcttcg
     3061 tccagcagga tcgagtcggc ttcgtcgatg acggccgcgg cgaacggccg caggacctgc
     3121 tcgccgggat ggagcgcgag tccatcgcgc aggtagtcga accccagctc gttggcggat
     3181 gcgtaggtga tatcgcgccg atacgccgcc cgccgttctt cggccgtcat ttcttgcgtc
     3241 acggctgcga cggacaggcc gagccagtgg taggtcttgc ccatccattc cgcatcgcga
     3301 cgagcgagat agtcgttcgc cgtcagcacg tgaacgcccc cgccggcgcg cgcgtaccac
     3361 gcaactgccg ggaccgccgc cagcgtcttg ccctcgccgg tctgcatctc cacgatccgt
     3421 ccgtgtgtca gcgccagagc gccttcgagc tgcgggtcga acatgtgcag cccgagcacg
     3481 cgcgacgcca cgacggccgt caccgccacg atctccggca cgcgcgtggc gctccgcgct
     3541 gcgcgcttca acgactcgtc gtccaggcgc tcgaagccgt cacgaagggt cctgacttcg
     3601 tcgatctccc gcggcacagg ctccgcacgc ggtgtacaca gcgaacgcca ccattcacgc
     3661 atggccgacc tccagcgggc gcgcgtggcg cgcccagtct tatgacaaca gttgaagaaa
     3721 ttcgactgga gcgatcaggc cgaggctggt ggcggccgct gaaacagcca gcgttcaacc
     3781 gggaaccgcc ggacgctgac ggaccggggc gcccgcgacg ggccggtggg agatggttgg
     3841 gagactcgtt gtgagagagg tcgcgtctcg tgttcggcct gctccggcgc cttcagccat
     3901 tcgatggaaa cgacgaggcg cagcgattgg agcgacctcg caggtcccgc cgccgcgcct
     3961 gcgaaggcga acacgacgac ggccagggag gccaggccga ggcggcgggt cacgaaggac
     4021 agtatacggc gaccgaggag acgatcgcga agctgaagga caagtaagcg gttgacaccc
     4081 ccaaggaaga cgcgtagcgt gtccctccag aaaggagcac cctcactatg cggttcgcct
     4141 ccatcaaata cgtgttcctc ctcgcccttc tcgtgctcac gtctctctcg aacccctcgg
     4201 cgcaacaggc gtcacagacg ggccagggcc catccctcgc atccatcgga ccgatcgtgt
     4261 tcggtcccga cggcacgctg ttcgctgccg acagtcaggg tgcgtccatc ttcgcgctgg
     4321 atctcggcgc gcaagccaac gcgggcgcgg ccaacgccaa ggggctcgac gccctcgacg
     4381 agaagctcgg cgccttgctc ggtaccgccg cgaaggagat cacaatcact gatcttgcgg
     4441 ttcatccgcg tacgcgaaac gcgtacgt
//
LOCUS       DBNGSH010000195         6982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1882826, whole genome shotgun sequence.
ACCESSION   DBNGSH010000195 DBNGSH010000000
VERSION     DBNGSH010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1882826"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC16CY_04410"
     CDS             <1..411
                     /locus_tag="AC16CY_04410"
                     /inference="COORDINATES: protein motif:HMM:NF047172.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3353054.1"
                     /translation="AIRRTAASVLAPWEKFLKAKFGLSTSLFNVLRILRGSHPTALTC
                     GEIGERTIARDPDVTRLVDQLIARGLVARTRSEKDRRIVEVSITKKGLELLQELEPHS
                     LQMPRALMGHVSATKLRRLATLLTEVLDGMGTYP"
     gene            534..1040
                     /locus_tag="AC16CY_04415"
     CDS             534..1040
                     /locus_tag="AC16CY_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3353055.1"
                     /translation="MSIAELGASNVQVTARSHKTLAAGEGLLRHSVPGETILYKVTGD
                     DTDGALDIFLLAIQPKSGPPLHIHHHQHETIYFTKGRYKVQIDDDVFSCEAGGFVYIP
                     MGARHAFMNVSDQPGECIVTFSPGATDKFFEEFAPVVRDGHPDPAKIAPIFAKHGWEL
                     VGPPLSAD"
     gene            complement(1317..1916)
                     /locus_tag="AC16CY_04420"
     CDS             complement(1317..1916)
                     /locus_tag="AC16CY_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353056.1"
                     /translation="MPGVVLTFFYLRRRYGWIRGSYRVLLFLTVVANVLIFTTDARVL
                     GPAIVPTSLFIAFAPPASTFLTRYPAWQRTKQRLHRRAVLLRDGTVQSLFGTPVHGAT
                     FPRITLWLAAVPWLSWLMGTLTLAGGGRTLGGQISIVITVVYVAAFYATLIVTPVLGY
                     LAVRYLRDMTGAARVWTLAGLLAGVKWCKDAYDAYSILL"
     gene            2501..2974
                     /locus_tag="AC16CY_04425"
                     /pseudo
     CDS             2501..2974
                     /locus_tag="AC16CY_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
     gene            3037..5091
                     /locus_tag="AC16CY_04430"
     CDS             3037..5091
                     /locus_tag="AC16CY_04430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3353057.1"
                     /translation="MSPEQAQGRTCDARSDIFSLGVVLYELLAGMRPFEGTSAVETLH
                     QIVHQPMPPLDEPTPVAVRMIVEKALEKDPDDRYQFARELAVDLRRAARRGDARVETV
                     VVAAASVPHGRRWLPVAAGMLLTAGALAVAAWWAWPRGGETLLRFEIAPPPGGQFVIG
                     GATFGGLALSPDGEQLAFVASVNSTALVWVRSLDDGTARPIAGTQSAQRPFWSPDGKS
                     IAYFGQEGLRRVGVPAGTNDLIVKIDTSLPCAGSWSSDDQILFNCGGSGIRVVPAYGG
                     EVKQLTENGGFPQALPGGAFLYLAPGPPFEVRAATIADPTGRRIMTADSPALFASGHL
                     LWRIGSALFAQPFDSAALKLSGEARRLLEPVASGSLADSILTVSATGRMVYDAAGNDL
                     QASWYDRSGKRLGPVGRAGSFQGVRLFDAGRRLMTQANAVGDRGLWLIDEQGRALRPI
                     AGDVTVSPTPSPDGKSVVFTVPTEKEWLMQRAGVAGDDRVTVLAPDPAAFKFVTDWAA
                     DLVLLSVVSQTSRIDIWSLRVNPDGTPAPGAVAKPYLQTLAQESDGRIAPAQSPRWVA
                     YVSDESGQVEVYLQSFPASGEKLAISSGGGGSPVWGPGGRELYYVTPDNTLMVVDVTL
                     GPTSASASSPRELFPLPFEGPAVSPPYDTLDGKRFVVLSPVAPATRPMKAITNWPALL
                     KQ"
     gene            5260..>6982
                     /locus_tag="AC16CY_04435"
     CDS             5260..>6982
                     /locus_tag="AC16CY_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3353058.1"
                     /translation="MALSPGTRLGPYEVTARIGVGGMGEVYQATDTKLKRQVAIKVLP
                     EALAHDAGRLARFQREAEVLASLNHPNIAAIHGLEERDGMTALVMEFVEGPTLADRIV
                     LGAIPVDEALPIAKQIAEALEAAHERGIVHRDLKPANVKVRPDGTVKVLDFGLAKAME
                     SGIPSGGAAGQDPSLSPTVTTPAVTQLGMVLGTAAYMSPEQAKGRPTDRRADVWAFGV
                     VLFEMLAGRRPFACDSVVETLGAVMHAEPDWGALGPGVPDSLRVVLRGCLQKDPKQRI
                     ADVQDVRLALAGAFAAAVAPRATGDGVEPAASRRWLPMAGAAALGGLVVALAASAFWP
                     AREAPPVDLFIITTPPDGPPGSGFGSDVDISPDGRSIVYGSGDDAESRLYLRRTDQLE
                     ATLLRGAAPATGPFFSPDGDQVGFFDPVARALKHVSVGGGPAETIVSNLGPGVVGASS
                     GRDGMIVYSSGNRLWQVPWQGGEPKLLASPPSNQSTGFLWPDVLPGGTGVLFTNYIAP
                     GNVRLAVFSRDTGKVSDLGPAGSQARYVETGHIVYAAAGTLRAAGFDQNRLALTSDPV
                     PVVEGALV"
BASE COUNT         1196 a         2197 c         2371 g         1218 t
ORIGIN      
        1 gccattcgcc ggacggcggc gagcgtactt gcgccgtggg agaagttcct caaggccaag
       61 ttcggcttgt ccaccagcct attcaatgtg ctgcgcattc tccggggcag tcatcccacg
      121 gccttgacct gtggcgagat cggcgaacgg acgatcgccc gcgacccgga tgtgacccga
      181 ctggtggacc agttgattgc gcgcggcctg gtggcgcgca cgcgtagcga gaaggaccgg
      241 cgcatcgtcg aggtgagcat taccaagaaa gggctcgagc tcctgcagga gctcgagccg
      301 cactccctgc agatgccgcg tgcattgatg ggccacgtca gcgcaacgaa gcttcgacga
      361 ctagcgacgc tgctgacaga agtgctcgac ggcatgggca cgtatccctg aaagagaagt
      421 tgtaagagaa gacggcaagg accacccggg caggcttgat atcagttgga acaaatattg
      481 tcgacacatg tatttggttt gacgtcagtt ttcccattca aggagaaccg cacatgtcta
      541 tcgccgaact cggagccagc aacgttcagg tgaccgctcg ctcccacaaa actctcgccg
      601 ctggtgaggg cctgctacgc cactccgtgc cgggagaaac catactgtac aaggtcaccg
      661 gcgacgatac cgacggggct ctcgatatct tcctgctcgc cattcaaccg aagagcggtc
      721 cgccactgca catccatcac caccagcacg aaaccatcta tttcacgaag ggccggtaca
      781 aagtgcagat cgacgatgac gtgttcagct gcgaggcagg cggcttcgtg tatatcccga
      841 tgggggcgcg tcatgccttc atgaacgtga gcgaccaacc gggtgaatgc atcgtcactt
      901 tttcgccggg cgctacggac aagttcttcg aagaatttgc gccggtcgtg cgcgatggtc
      961 atccggaccc cgcgaagatc gctcccatct ttgccaagca cggatgggag ctggtcggtc
     1021 ctccgctgtc ggccgactag cggagctgcc agatggagca tctggcgata cgtcggcgct
     1081 tctacgtcgg cacgagcgtg gtcaggcggg cgcggacgat gttaatccgt gcccgtcagt
     1141 gacttcctaa acaggaattc ctaatctcag ccgttcgccg tggccctgac accgcctccc
     1201 cggatgacgc gactattcgg ctctacaacg cccgaagaac atgcggtcgc gtgcctgcgg
     1261 acgatcctgt agagtcgcga cgggacggat tcaccgatgc tgagagcttc gacgctctac
     1321 agaaggatcg aatacgcgtc gtaagcgtcc ttgcaccact tcacgcccgc gaggagaccg
     1381 gcgagcgtcc acactcttgc ggcgccagtc atatcgcgga gatagcgaac cgccagatag
     1441 ccgagcaccg gtgtcacgat gagcgttgcg tagaatgcgg cgacgtagac aaccgtgatc
     1501 acgatagata tctgaccgcc gagcgtgcgg cctccgcccg cgagcgtcag cgttcccatc
     1561 agccaggaca gccagggcac tgccgcgagc cacaacgtga ttcttggaaa cgtggcgccg
     1621 tggactggcg taccgaacaa cgattggact gttccatcgc gcaggagaac cgcacggcga
     1681 tgaagccgtt gcttggtccg ttgccacgcg gggtagcgtg tcaggaatgt gctggccggc
     1741 ggggcgaagg cgatgaagag acttgtaggc acgatagctg ggccgagaac ccgtgcgtcg
     1801 gtggtgaaga tcagaacgtt ggcgacgacg gtcagaaaca acagcacgcg atacgaaccg
     1861 cggatccacc cgtaccgccg tcgcagatag aagaacgtca gaaccactcc aggcacagcg
     1921 atcccaccgg tgaccacgaa ggcgaaaaag ccaagcgtgg ccagccggtc gactcggcgc
     1981 ggtgggacga gcgggttcgg gccgtcggtc gcgacgatgg cgaacagtat gccggccgtg
     2041 gcgagcgcgg ccccaaggaa gaccgtgttg cggatgatgg cttttatcag gcatttagac
     2101 tatcggcaga ttggccggat tgtattggaa caaggttatg acgccagatt ctcccgccgc
     2161 accgaagccc atgatccgcg tgatcatgca atctcacgta atgctcaccg tctgatctgg
     2221 acgtagcggc ggccagtggg cgatccttcc gtatccaccg gcaggaggga aaccatcgaa
     2281 ctcattggct tcgacatcaa gcagccgccg aagccgaatt acgccagagc ggatgcgcta
     2341 gcagcacgac gctgcttgtg gtggaagtat tttttggtac cggtacgggg ttttgcgaat
     2401 ggttggtgcc tgaagttcga gggaatcgcc gcctgacccc ggtagccgtc ggaaattggt
     2461 gtatccccgt tcgtggttct ggcgcacact gtctgccgtg atggcgaggc ttgcggtggg
     2521 ggccacgatc gggaactacc ggattgaggg cgtgctcggc gaaggtggca tgggtatcgt
     2581 ctaccgtgcg accgacacca acctggggcg ccctgtcgcg atcaaggtcc tctcgtccga
     2641 gattgccgat gccagcgccc gccgccgctt tcagcgcgaa gcgcaggcgg cgtcgtcgtt
     2701 gaaccatccc gcgatcctga cagtccacga cgcgggcgag tcggagggtc ggcagtatct
     2761 cgtcaccgag ctggtggacg gcggtacgct tcgcacgtgg gccaacgccg ggcgatcgtg
     2821 gcgccagacg gtcgaactca tcgcctcgat cgccgacggc ctggcgaccg cgcatggtgc
     2881 gcagatcctg caccgcgaca tcaagccgag caacatcctc atgtcggcca gcggccaccc
     2941 gaagctggcc gacttcggtc tcgcgcggct caacgctccg gctgccgacg atcagacggt
     3001 cacacaggcc ggggtcattc ttggcacccc gcgtacatgt cgcccgaaca ggcgcagggc
     3061 cggacctgcg atgcccgaag cgacatcttc tcgctgggcg tcgtgctcta cgagctgctg
     3121 gcgggcatgc ggccgttcga ggggacgtcg gccgtggaga cactccacca gatcgtccat
     3181 cagccgatgc cgccgctcga tgagccgacg ccggtcgccg tgcgcatgat cgtcgagaaa
     3241 gcgctcgaga aggaccccga cgatcgctac cagttcgctc gcgagctagc ggtggacctg
     3301 cggcgcgcag cgcgccgggg cgacgcgcgt gtagagaccg ttgtcgtggc cgccgccagc
     3361 gtgccgcatg gccggcgatg gttgccggtc gcggccggta tgctgctgac ggccggagcg
     3421 ctggcggtgg cagcgtggtg ggcctggccg cggggcggcg aaacgctgct gcgcttcgaa
     3481 atcgcgcctc ccccgggcgg ccagttcgtg attggcggcg cgactttcgg cggcctcgcc
     3541 ttgtcacccg atggggagca gctggcgttc gtcgcctcgg tcaacagcac ggccttggtc
     3601 tgggtgcgtt cactggacga tgggaccgct agaccgattg ccggaaccca gtccgctcag
     3661 cgtccgttct ggtcaccgga cggcaagtcg atcgcctact tcggccaaga agggctgcgg
     3721 cgcgtgggcg tgcccgccgg caccaacgat ttgatcgtga aaattgatac gagtctccca
     3781 tgtgcgggct cgtggtccag tgacgatcag atcttgttca actgtggggg gagcgggatt
     3841 cgtgttgtgc cagcgtacgg aggcgaggtt aagcaattga ccgagaacgg tgggtttccg
     3901 caggcgctcc ccggaggggc cttcctctac ctcgcgcctg gcccaccctt cgaagtgagg
     3961 gccgcgacga tcgccgatcc gacggggcgg aggatcatga ctgccgacag cccggcgttg
     4021 tttgcctctg gccacttact ctggcgcatt ggcagcgccc tcttcgcgca gccgttcgac
     4081 tccgccgcgc tcaagttgag cggtgaggcg cggcggctgc tggaaccggt tgcgtccggc
     4141 agcctggccg actcgattct caccgtgtcg gccaccggcc ggatggtcta tgacgccgct
     4201 ggaaacgact tgcaggcctc gtggtacgac cgaagcggca agcggctggg gccagtcggc
     4261 cgcgccggca gcttccaggg cgtgcggctg ttcgacgccg gccgccgcct gatgacgcag
     4321 gcaaacgccg taggggatcg cggcctctgg ttgatcgacg aacagggtcg tgcgctccgc
     4381 ccaatcgccg gcgacgtcac ggtgagcccg acgccgtcac ccgacggcaa gtcggtggta
     4441 ttcacggtcc cgactgaaaa agagtggctg atgcagcggg ccggggtggc cggcgacgat
     4501 cgggtcaccg tgctggcgcc cgatccggcc gcgttcaagt tcgtcaccga ctgggctgca
     4561 gacctggtgc tgttgagcgt ggtgtctcaa acgtcgagaa tcgacatctg gtcgctccgg
     4621 gtgaatccgg acggcacgcc tgcgccgggt gcggtcgcca aaccgtacct gcagacgctc
     4681 gcgcaagagt cggacgggcg gattgctcct gctcagtcgc cgcgctgggt ggcgtacgta
     4741 tcggacgagt ccggacaggt cgaggtctac ctgcagtcgt tcccggcgag tggcgagaag
     4801 ctggcgatct cgagcggtgg tgggggatct ccggtttggg gacccggagg ccgcgagttg
     4861 tactacgtga cgccagacaa cacgctgatg gttgtggacg tgacgctcgg cccgacgtcg
     4921 gcgtcggcgt cgtccccacg cgagctgttt ccactcccgt tcgaggggcc ggcagtgtca
     4981 ccgccgtacg acacgttgga cggcaagcgg ttcgtggtct tgtcgcctgt ggcgccggcg
     5041 acgcggccga tgaaggcgat caccaactgg ccagctcttt tgaagcagtg acgctccgct
     5101 agtcatgcgc gaaatcacta ctacgacccc ggcgtgattc ttctgggtac cggtacgggg
     5161 atttgcgaaa ggttggatca tcaagttcgc aggaatagcc gcctgatccg acatgagccg
     5221 agtgtcactt catagccgcg ctagaatctc atcactccct tggcgctgtc accaggcacc
     5281 cgcctcggtc cctacgaggt caccgcccgg atcggggtcg gtggcatggg cgaggtctac
     5341 caggcgaccg acacgaagct gaagcgccag gtcgcgatca aggtgctccc ggaggccctg
     5401 gcgcacgatg ccgggcggct ggctcgtttc cagcgagagg ccgaggtcct ggcgtcgctc
     5461 aatcacccga acatcgccgc catccacggc ctcgaggaga gagacggcat gaccgcgctg
     5521 gtgatggagt tcgtcgaggg accgaccctc gccgaccgca tcgtcctggg ggccattccg
     5581 gtcgacgagg cgcttccaat cgccaaacag atcgccgagg cgctcgaggc cgcgcacgag
     5641 cgaggcatcg tgcaccgcga cctgaagccg gcgaacgtga aggtgcggcc cgacggcacc
     5701 gtgaaggtgc tggacttcgg cctcgcgaag gcgatggagt ccgggattcc ttcgggcggc
     5761 gcggcggggc aggacccgtc gttgtcgccg acggtcacga caccagccgt gacgcaactg
     5821 ggcatggttc tggggacggc agcctacatg agccccgagc aggcgaaggg ccggcccacg
     5881 gacaggcgcg ccgacgtgtg ggcgttcggc gtcgtgctgt tcgagatgct cgccgggcgg
     5941 cgcccgttcg cgtgcgactc cgtcgtcgag acgctgggcg cggtcatgca cgccgaaccg
     6001 gattggggcg cgctcggtcc gggcgttccc gactcgcttc gcgtcgtgct gcgtgggtgc
     6061 ttgcagaagg acccgaagca gcgcatcgcg gacgtccagg acgtgcgcct ggcgctggcc
     6121 ggcgcctttg cggcggcagt ggcgcctcgc gcgacggggg acggcgtcga gcctgctgcc
     6181 tcccggcgct ggctgccgat ggctggcgcg gcggccctgg gcgggctggt cgtcgcgctc
     6241 gcggcgtcgg ccttctggcc ggcgcgagag gcgccaccgg tggacctttt catcatcacc
     6301 acaccgcccg acggtccgcc cggcagcggg tttggttccg acgtggacat ctctccagac
     6361 ggccgatcca tcgtctacgg ctccggtgac gacgcggagt cgcggctcta cctgcgccgg
     6421 acggatcaac tggaggccac cctgctcagg ggcgctgcgc cggccacggg ccctttcttc
     6481 tcgccggacg gcgatcaggt cggcttcttt gatcctgttg cacgtgcgct gaagcacgta
     6541 tccgtgggcg gagggcccgc cgaaaccatt gtctccaacc ttggaccggg tgtagtcggc
     6601 gcgagttcgg gccgggacgg catgattgtg tacagctcgg ggaacaggct ctggcaggtg
     6661 ccctggcagg gtggcgagcc caagcttctg gcgagccctc cttctaatca gtccacgggt
     6721 ttcctctggc cggacgtgct gccgggcggg acgggcgtgc tcttcaccaa ttacatcgct
     6781 cccggcaatg ttcggcttgc cgtgttctcg cgcgacacgg gcaaggtgtc cgatctcggc
     6841 ccggcgggca gccaggcccg gtacgtggag accggccaca ttgtctacgc cgccgccggc
     6901 acgctgcggg cggccggctt cgatcagaat cggctggcgc tcacgagcga tcccgtgccg
     6961 gtggtggagg gcgcgctcgt ca
//
LOCUS       DBNGSH010000196        10234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3580819, whole genome shotgun sequence.
ACCESSION   DBNGSH010000196 DBNGSH010000000
VERSION     DBNGSH010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3580819"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="AC16CY_04440"
     CDS             complement(<1..521)
                     /locus_tag="AC16CY_04440"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR3353059.1"
                     /translation="MKRALRSWLWRVPVDQEVDEEIAFHVEMRTRELVDKGLDAKAAR
                     EVVLARIGDTGRLRRTCIDLGRKRDREMRLTQWLADLRDDVAVAVRQLKASPAFTTVA
                     VLTLALGIGANSAMFAVADATLLRPLPYPGGDRVVAVSELRRDGTRGPANPLDFVDWR
                     ERNRAFEAMAGVM"
     gene            complement(590..3265)
                     /locus_tag="AC16CY_04445"
     CDS             complement(590..3265)
                     /locus_tag="AC16CY_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3353060.1"
                     /translation="MKRSLRSWLWRVPIDQEVDEEIAFHIEMRTRELVDKGLDPGIAR
                     TMVRARLGDAARLKRTCVDLGRRRDREMRLTRWLEEFRDDVRFALRQLKMSPGFTLVA
                     TITLALGIGANSAMFALADAALVRPLPYPEPDRLVALSEIRQGQPGSAVNPVDFVEWS
                     ERTRSFTAIAAVVRGGGSLVGDDGVAEPVPGQAVTSRFFDVLGVKPIAGRTFRDDDEG
                     PVSDVVVLSEGFWRRRFGADPSLVGRAARLSGRTFTVIGIVPADFQFELPGTTSEAPS
                     MMWTLLNRPEDRGPAQRYPHYLQVIGRLKDGVTLNTARADIAGVADAIAREMPGTNKG
                     HAATASPLRERIIRSELRLTSILLLGVVGLVLLMCCANVANLLLARASARSREFAIRS
                     ALGAGRRRIVRQVLTESLVLAILGGLTGAAFGATIVRAAPSLIPPDLIPGVVTVSFDG
                     RVLTFCALAALVVAVLYGLSPAWHIARMPPVQAMTNDGRSVTGSSARFRRALAVSQVA
                     IAVFLLCGAGLLLRSLLALESVDSGSRTGDVLTMIVGAGSGADTSPASMWRKYQAYQR
                     EVEQVPGVRSVAWGGALPFGGMWYDQNFQIDGDQPRPPADRDGAGYQIVSPSFLPLLG
                     VAVLEGRGLTKGDTAESPQVCLVDEEFVRRYLRGRPVLGTRISINAMVQPPQAVTREI
                     VGVVGQVKERPDEPQPRPHVYVPLAQNPWWSATLVVQPIDGPAEALTSGVRAALARVD
                     REQPATRIRTLSAIGAEATARPRFRAVLIGTFASLALVLAMVGVFGVLAYSVQQRTRE
                     FGVRIALGASATNLLGLVLGNAARVIGTGVVIGLMCAFAFAQSVASFLFGVQPRDPVT
                     FGSAGLVLLLTAMVACAVPALRAVRVDPVVAFRSE"
     gene            complement(3262..3597)
                     /locus_tag="AC16CY_04450"
     CDS             complement(3262..3597)
                     /locus_tag="AC16CY_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3353061.1"
                     /translation="MPFKTPNALLHGTLDALILKTLTGGPRHGYAIAKFIEDASDETV
                     VVEEGSLYPSLYRMERRGWLEAEWGESELGRRAKLYRLTRQGRAQLAAETDAWRQFAA
                     GVSKILLAQ"
     gene            complement(3733..4836)
                     /locus_tag="AC16CY_04455"
     CDS             complement(3733..4836)
                     /locus_tag="AC16CY_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3353062.1"
                     /translation="MRRLLSFLVLSSAVALTFAQTRSTLDIYLVDVEGGNATLFVAPS
                     GESLLIDTGNGGQAATRDADRIMAAAKDAGLRQIDRLVTTHYHGDHFGAMSELASRIP
                     VREFIDHGANVQPNAATDAFLKDVYPKLYANARHTVAKPGDRIAVSGLDIRVVASAGE
                     GIRDALPGGGQRNPYCSAHQAKDADASENAQSVGSRITFGRFRALHLGDLTWNKEHDL
                     VCPNNRLGTVDLFIVTHHGQPVSNAPVLVHAVAPRVAIMNNGTRKGGQPDAMKVLFSS
                     PGLEDLWQLHFSQLSGQDYTVPGVFIANTVDDQPTSMPIAALTPPAPGQQGAAPPPVH
                     NGPAHWIKVSARDDGSFTVTNARNGFSKTYAAR"
     gene            4884..5558
                     /locus_tag="AC16CY_04460"
     CDS             4884..5558
                     /locus_tag="AC16CY_04460"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017543361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR3353063.1"
                     /translation="MHIDIWSDIVCPWCYLGKRRFERALEGFERRDEVRVTHRSFQLD
                     PAKPRGTTSSRRTMLMSKYRLSDDQVRTMDARMEQTAAADGLEYHLTDAGLTGNTLDA
                     HQLVHLANSLGRADAMIERLYRAYFTEQRSVFDHASLVDLAAEAGVGADEARQALERD
                     LFVDAVTRDLNEARSLGITGVPFFVFDGRLGVSGAQAIEVFTQALASLIPQKSTVTFQ
                     RTNRGV"
     gene            complement(5513..8641)
                     /locus_tag="AC16CY_04465"
     CDS             complement(5513..8641)
                     /locus_tag="AC16CY_04465"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3353064.1"
                     /translation="MRRASKVVLIPAAILAAVLLPSVLSAQGTLTGTVRDQSSAVLPG
                     VTVEASSPALIEKVRAAVTDGAGQYRITGLNPGTYSLTFTLTGFIVVKREGIELSGTA
                     TLTIPVDMRVGSLAETITVTGESPVVDLQTTQRETVISADVVSAIPGNRSVGTLLNAV
                     PGLVVNDGALAASPTMTFFAARGGPINEGRMAINGMTIAAPFNGGGVSTYILDSVNVD
                     EISVAVAGGLGESDIGGPVMNLVPRSGGNQFRGSAFINNAGDWSRGNNLTPELMAPPP
                     GPNLKETPGIIGSYDTSVSYGGPIMRDRLWFFGSYRKLDTAASVPGAVYNANAYNASR
                     WDWVGDPTVTSRTLQGRESYIGRITAQVSARHRLSYNQEYQRRCEGSTLKVDSDNGCN
                     TRGADWVAAGTAAASPEANPNYFGNTPYHVNQVMWTAPMTGKLLLEAGFTRFMFRGGT
                     TGRPSPDGIMDLIPVTEQSTANNPLTGLPYAPRALYAYRGVATANPNYANPNNWNASA
                     SYVTGSHAVKAGYQGAYIRVNNWFLVNEPQLAYRFNQGVPNQFTFRLPEWHQSDRTGT
                     AALYIQDKWTRGRLTLQGALRYDRAWSFMPAEFNGTDETSPFNAAPISFPRTAGVDSF
                     NDITPRFGAAYDVFGNGKTALKFNIGHYLDAATNDSEYTSNSPAARIVRTGTRNWDDR
                     FYPAGDSRRDNKAIDCDIMNFAANGECPAATGDTLNFGQASGNITQVNQETLRGWGVR
                     QNDWQWGVTLQQEVIPRVSAEVAYNRRWFHGLKVTDNTRRGPGDYEQFTITAPSDPRL
                     PGGGGYPITLSLVTQAGSDRGVQNYVTFETDFGPERESYWDGVDFTLNARLRQGLVLQ
                     IGTQTGRSVEDNCATASVIDGGGLIKDVRNCRDVDPFQTTVRGLASYTVPKIDVLVSG
                     TVRSQPPFERVANWAFPNILIRDAIGRLPPGSTATGTTTVDVLDADHRLYADTRRTQI
                     DMRFAKILRFGSTRVDVGVDLSNLLNTNYPTTYENAYQFSVGNTQQGGTWNNPTAVYT
                     PRFVRWNVTVDF"
     gene            complement(8766..9404)
                     /locus_tag="AC16CY_04470"
     CDS             complement(8766..9404)
                     /locus_tag="AC16CY_04470"
                     /EC_number="1.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008873 - gluconate 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0008875 - gluconate dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HIR3353065.1"
                     /translation="MVTRREAIQRVTVLLGGAALVGGEQLLEAAPAAGAAQRAAAARG
                     VGTFSAADVAFLDEVAETILPETGTPGAKAAATGAFMALMVTDTYTHRQQAIFRDGMR
                     QMDEASRREFTAPFLQARPDQRLALLRRLDAEQQAETDAREAALTSRAPAAPPPGDAP
                     VHYFRLMKELALLGYFTSEIGYRQAMRYVETPGRYDPCAPYKPGEKIWAPHA"
     gene            complement(9404..>10234)
                     /locus_tag="AC16CY_04475"
     CDS             complement(9404..>10234)
                     /locus_tag="AC16CY_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR3353066.1"
                     /translation="QVTEYSAPVIFLCASALNSTWILMRSATDVWPGGLGSSSGELGH
                     NLMDHHFRCGANGEIEGHEDSYVYGRRPTGFYIPRFRNLPGDERREYLRGFGYQGSAS
                     RQGWERAVAELGVGGAFKDAMATPGPWRIGATAFGEMLPNHGNRVWIDARTDKWGLPV
                     LAIDCATGENERLMRRDMMNDMAEMLEAAGVRDVRVYDNDYAPGMGIHEMGTARMGRD
                     AKTSVLNAHNQVWDCPNVFVTDGACMTSSACQNPSLTYMALTARAAAYAVEELKRGNL
                     "
BASE COUNT         1721 a         3424 c         3323 g         1766 t
ORIGIN      
        1 ctcatcacgc cagccatcgc ctcgaacgcg cgattgcgct cgcgccagtc gacgaagtcg
       61 agcggattgg ccgggccgcg cgtcccgtcg cgtctgagct cggacacggc caccacccga
      121 tccccacccg gatagggcag cggacgcagc agcgtcgcat cggcgacggc gaacatcgcg
      181 ctgttggcac cgataccgag cgcgagcgtc aggacggcca ctgtcgtgaa cgcgggcgat
      241 gccttcagct gccgcaccgc aacggcaaca tcgtccctga ggtctgccag ccattgtgtc
      301 agccgcatct cgcgatctcg ctttcgtcca aggtcgatgc aggtgcgcct gagtcgaccg
      361 gtatccccga ttcgcgcgag caccacctcg cgcgccgcct tggcgtccag ccccttgtca
      421 accagctcgc gcgtccgcat ctcgacgtgg aaggcgatct cctcatcgac ctcctggtca
      481 acaggcacgc gccacagcca cgatcgcaac gctcgcttca tatgctcaat tgatgatctt
      541 tgattggtga tctgtgaatc gttgctgatt gtcgactcac aactcctctc tattcactcc
      601 tgaacgccac caccggatcc acccgcacgg ctcgcagcgc gggtaccgca cacgcgacca
      661 tcgccgtcag taacagcacc aacccggcgg acccgaacgt caccggatcg cgcggctgga
      721 caccaaagag aaacgacgcc accgactgcg caaacgcgaa ggcacacatc aacccgatga
      781 cgacgcccgt tccgatgacg cgcgcggcgt tccccagtac gaggccgagc aggttcgtgg
      841 cgctggcgcc aagcgcgatg cggacgccga actcacgcgt gcgctgctgg accgagtacg
      901 ccagcacacc aaacacgccg accatcgcga gcaccagcgc cagagacgca aaggttccaa
      961 tgagcacggc tcgaaagcgc ggcctggcgg tggcttctgc gccgatcgct gacagcgttc
     1021 gaatgcgggt cgcgggctgc tcccggtcaa ctcgtgccaa tgccgctcga acgcccgatg
     1081 tcagcgcttc tgccggcccg tcgatcggct gaacgaccag ggtggcgctc caccacgggt
     1141 tctgcgcgag cggcacgtag acgtgaggcc gtggctgtgg ctcgtcaggc ctctccttca
     1201 cctgccccac gactccgacg atctcccgtg tcactgcctg cggcggctgc accatcgcgt
     1261 tgattgagat gcgcgtcccg agaaccgggc gaccccgaag gtagcgacgt acgaactctt
     1321 cgtcgacgag gcagacctgg ggactctcgg cggtgtcacc cttggtgagg ccacgccctt
     1381 cgagcacggc aacaccgaga aggggcaaga aggacgggct cacgatctgg taaccggctc
     1441 catcgcggtc cgcgggcggg cgtggctggt caccgtcgat ctgaaagttc tggtcgtacc
     1501 acataccacc gaatggcagt gcgcctcccc aggcgacaga acgcactcca ggcacttgct
     1561 cgacttcgcg ttggtaggct tggtacttcc gccacatcga tgcggggctg gtatccgctc
     1621 ctgacccggc gccgacaatc atggtgagca cgtcgcccgt gcgagagcca gagtcaacgc
     1681 tctccagcgc cagcagcgag cgcagcagca agcccgcccc gcacaagagg aagacagcaa
     1741 tggcaacctg actgacggcg agcgcacggc gaaaacgagc gctggatccg gtaacggagc
     1801 gcccgtcgtt cgtcatcgcc tgcaccggcg gcatccgggc gatgtgccac gccggcgaca
     1861 gaccgtagag caccgcgacc acgagcgcag ccagcgcgca gaacgtgagc acccggccgt
     1921 cgaagctgac cgtcaccacc cccgggatca gatctggcgg gatgagtgat ggcgcggccc
     1981 gcacaatcgt cgcaccgaac gcggctcccg taagaccgcc gaggatcgca aggacgagac
     2041 tttctgtcag gacttgtcga acgattcgtc gtcgtccggc gccgagtgcc gaccgtattg
     2101 caaactcacg cgaccgggcg gaggcacggg caagcaggag gttggcgaca ttggcgcagc
     2161 acatcaggag cacgaggccg acgacgccga gaagcagaat cgacgtgagc ctcagttcgc
     2221 tgcggatgat tcgctcgcgc agcgggctgg ccgttgcagc gtgtccttta ttggtgcccg
     2281 gcatctctcg cgcaatcgcg tcggcgacac ccgcgatgtc agcgcgcgct gtgttcagag
     2341 tcacgccatc tttcaatcgt ccgatgacct gcaggtaatg tggatagcgt tgagccggac
     2401 cgcggtcctc tggtcggttt agtagcgtcc acatcatgct cggggcttcg cttgtcgtgc
     2461 ctggaagctc gaactgaaag tctgccggca cgatcccgat gacggtgaac gttcgtccac
     2521 tcaatcgagc cgcgcgacca acgagcgacg gatcggcgcc gaaccggcgg cgccagaatc
     2581 cctcgctcaa caccaccaca tcggacacgg gaccctcgtc atcgtcccga aacgtgcgac
     2641 ctgcgatcgg cttcacaccc aacacgtcga agaagcgact ggtgactgcc tgaccaggca
     2701 ctggctccgc gacaccgtca tccccgacga gggagcctcc gcccctgacg acggcagcga
     2761 ttgccgtgaa gctgcgtgtc cgctccgacc actcgacgaa atcgaccggg ttgaccgcag
     2821 agcctggctg accctgccgg atctctgaca gcgcgacgag tcggtccggc tcgggatacg
     2881 gcagcgggcg gaccaacgcg gcgtcggcca gcgcaaacat cgcgctgttc gcgcctatgc
     2941 cgagcgccag cgtgatcgtc gccaccaacg tgaagccagg cgacatcttc agttgacgga
     3001 gtgcgaaccg cacatcgtcc ctgaattctt caagccaccg tgtcagtcgc atctcccggt
     3061 ccctccgtct cccaaggtct acacatgtgc gcttgaggcg cgcagcatct ccgagtctcg
     3121 cgcgcaccat cgtgcgagcg atccccgggt ccagcccttt gtcgaccagc tcacgcgtgc
     3181 gcatctcgat gtgaaaggcg atctcctcgt ccacctcctg atcgatcggc acgcgccaca
     3241 accacgagcg aagggagcgt ttcattgggc cagcagaatc ttggaaaccc ccgcggcgaa
     3301 ttgccgccag gcatcggttt cggccgccag ttgtgcccgc ccctgccgtg tgaggcgata
     3361 aagcttggcc cggcggccga gctcggactc gccccactcc gcctccagcc accctcgacg
     3421 ctccatgcgg tacagggacg gataaagcga cccctcctcc accacgaccg tttcgtcgga
     3481 cgcgtcttcg atgaacttgg cgatcgcgta gccgtggcgg ggtccacccg tcagggtctt
     3541 cagaatgagg gcgtcgagcg tcccgtgcag cagcgcgttg ggcgtcttga aaggcatgtc
     3601 gccggctcca ttaccaagac tttctgggta atagaccctt tgacgacgat gggtggccga
     3661 tggttggctg cgggtccggc tcaagccgga cactacgtac ggacagacgt agcgtccggc
     3721 ttcagaccgg tatcagcggg cagcgtaggt cttgctgaac ccgttccgtg cgttggtgac
     3781 cgtgaaagac ccgtcgtcgc gggcggacac cttgatccag tgtgccggtc cgttgtgcac
     3841 gggcggcggg gctgctccct gctgccctgg cgccggcggc gtcagcgcgg caatcggcat
     3901 ggacgtgggc tggtcgtcga cggtattggc gatgaagaca ccaggaaccg tgtaatcctg
     3961 gccgctcagc tgagagaagt ggagctgcca caggtcctcg agcccgggcg acgagaagag
     4021 tactttcatg gcgtccggct gtcctccctt gcgggtgccg ttgttcatga tggccaccct
     4081 cggcgcgacc gcgtgcacga gcacgggcgc gttcgacacc ggctggccgt ggtgcgtcac
     4141 gataaacagg tccaccgtcc ctaatctgtt gttcgggcag accaggtcgt gctccttgtt
     4201 ccatgtcaga tcgccgaggt gcagcgcccg gaaccggcca aaggtaatgc ggctgccgac
     4261 cgactgcgcg ttctcgctcg cgtcagcgtc cttcgcctgg tgcgcagagc aatagggatt
     4321 cctctggccg cctcccggca gcgcgtcgcg aatgccttcg ccggcggacg cgacgacgcg
     4381 gatgtccagc cccgaaacgg cgatccggtc gcccggcttc gcgaccgtgt gcctggcgtt
     4441 ggcgtacagc ttcggataga cgtctttcag gaacgcatcg gtcgccgcgt tgggctggac
     4501 gttggcgccg tggtcgatga actcccgtac cgggatgcgc gacgccagct cgctcatcgc
     4561 gccgaagtga tcgccgtgat agtgtgtcgt gacgaggcgg tcgatctgac ggagaccggc
     4621 gtctttggcc gccgccatga tccgatccgc gtcgcgcgtc gccgcctgac ccccgttgcc
     4681 ggtatcgatc agcagcgatt cacctgacgg cgcgacgaag agcgtggcgt tgcccccctc
     4741 gacgtcaacg aggtagatgt cgagcgtcga gcgcgtctgg gcgaaggtga gagcgacggc
     4801 ggacgacaga acaaggaacg acagcaatcg gcgcatggcc ggccctcctg aaagcaagcc
     4861 cacgtaggct aacaggtttt ggcatgcaca tcgacatctg gtccgacatc gtctgcccct
     4921 ggtgctacct gggaaaacgc cgcttcgagc gggcactcga ggggttcgag cggcgcgacg
     4981 aggtccgcgt gacacatcgg tcgttccagc tcgatccggc gaaacccagg ggtacgacct
     5041 cgagccgccg gacgatgctg atgtcgaagt atcgcctctc cgacgatcag gtgcggacga
     5101 tggacgcgcg gatggagcag acagcggcgg cagatggcct cgaataccac ctgaccgacg
     5161 cgggcctgac gggcaacacg ctcgacgccc atcagctggt gcatctggcg aacagcctcg
     5221 gacgggccga cgcgatgatc gagcggctct accgcgccta cttcaccgaa cagcgctcgg
     5281 tgttcgacca cgcgtcattg gtcgatctgg ccgccgaggc gggtgttggc gcggacgagg
     5341 ctcgtcaggc gctcgagcgc gacctcttcg tggacgccgt gacgcgcgac ctgaacgagg
     5401 ccaggtcgct cggcatcacg ggcgtcccct tcttcgtctt cgacggacgg ctcggcgtct
     5461 cgggcgcgca ggccatcgaa gtcttcaccc aggctctcgc ctccctaatc cctcagaaat
     5521 ccacggtcac gttccagcgg acgaatcgcg gcgtgtagac cgccgtgggg ttgttccacg
     5581 tgccgccctg ctgcgtgttg ccgacgctga actgataggc gttttcgtac gtcgtcgggt
     5641 agttggtgtt caacaggtta ctgagatcga cgccgacatc cacgcgcgtc gatccgaatc
     5701 gaaggatctt cgcgaagcgc atgtcgatct gcgtccgtcg agtgtcggca tacaggcgat
     5761 ggtcggcatc caggacgtcg acggtcgtgg tgccagtcgc ggttgatccc ggcggcagcc
     5821 gcccgatggc gtcccggatc aggatgttgg ggaacgccca gttcgccacg cgctcgaacg
     5881 gaggctgcga gcgcaccgtc ccgctgacga gcacgtcgat ctttggcacc gtgtacgatg
     5941 ccaacccccg gaccgtggtc tggaacggat cgacgtcgcg acagttgcga acgtccttga
     6001 tcaggccgcc cccgtcaatc acgctcgcgg tcgcgcagtt gtcctcgacg gagcgcccgg
     6061 tctgcgtccc gatctgcagt accaggccct gtcggaggcg cgcgttcagc gtgaaatcga
     6121 caccgtccca gtacgactcg cgctccgggc cgaagtccgt ttcgaatgtg acgtagttct
     6181 gcacgccgcg atccgagccc gcctgcgtca ccagcgacag cgtgatcgga tagccgccgc
     6241 cgccgggcag gcgcggatcc gatggggcgg tgatcgtgaa ctgctcatag tcgcccgggc
     6301 cgcgccgggt gttgtcagtc accttgaggc cgtggaacca ccgtcggttg tacgcgacct
     6361 ccgctgatac gcgcgggatc acttcctgct gcaacgtgac tccccactgc cagtcgttct
     6421 gacgcacgcc ccagcctcgc agcgtctcct ggttcacctg cgtgatgttg cccgaggcct
     6481 ggccgaagtt gagcgtgtcg ccggtcgccg ccgggcattc gccgttggcc gcgaagttca
     6541 tgatgtcgca gtcgatggcc ttgttgtccc gccgcgaatc gccggcgggg tagaagcggt
     6601 cgtcccagtt gcgggtgccc gttcggacga tgcgggcggc ggggctgttg ctcgtgtatt
     6661 cgctgtcgtt cgttgcggca tcgaggtagt ggccgatatt gaacttcagc gccgtcttgc
     6721 cgttgccgaa cacgtcgtag gcagcaccga agcgcggcgt gatgtcgttg aacgaatcga
     6781 cgccggcagt ccgcgggaag ctgatcggag ccgcattgaa cggtgacgtc tcgtccgttc
     6841 cgttgaactc cgccggcatg aagctccagg cgcggtcgta gcgcaatgcg ccctgcaagg
     6901 tcagccggcc acgcgtccac ttgtcctgga tgtacagcgc cgccgttccc gtgcggtccg
     6961 actggtgcca ctccggcagg cggaacgtga actgattcgg cacgccttga ttgaagcggt
     7021 aggccagctg cggctcgttg acgaggaacc agttgttgac ccggatgtac gcgccctggt
     7081 agccagcctt cactgcatgc gagcccgtga cgtacgaggc cgaggcgttc cagttgttcg
     7141 ggttggcgta attcgggttg gcggttgcca cccctcgata ggcatagagc gcgcgcggcg
     7201 catacggcag acccgtcaac ggattgttcg ccgtggactg ctcggtcacc gggatcaggt
     7261 ccatgatgcc gtccggcgat ggccggcccg tcgttccgcc gcggaacatg aagcgggtga
     7321 agcccgcctc gagcagcagc ttgcccgtca tcggcgccgt ccacatcacc tggttgacat
     7381 gatacggagt gttgccgaag taattcggat tggcctcggg cgacgccgcg gccgttcccg
     7441 ccgcgaccca gtcggcgccg cgcgtgttgc agccgttgtc gctgtcgacc ttcagtgtcg
     7501 agccctcgca gcggcgctgg tactcctggt tgtaggagag gcgatgtctg gcggacacct
     7561 gcgccgtgat cctgccgatg tacgactcgc gcccctgcag cgtccgcgag gtaacggtcg
     7621 ggtccccgac ccagtcccac cgcgaggcgt tgtaggcatt cgcgttgtac acggcgcccg
     7681 gcaccgacgc cgcggtatcc aatttgcgat agctgccaaa gaaccagagt cgatcgcgca
     7741 tgattgggcc gccgtaggag acgctcgtgt cgtaggagcc gatgatgccg ggcgtttcct
     7801 tgagattcgg tcccggaggg ggcgccatca attccggagt caggttgttg ccgcgcgacc
     7861 agtcgcccgc gttgttgatg aaggccgagc cgcggaactg gttgccgccg gatcgcggca
     7921 cgaggttcat caccggcccg ccgatatcgg actcgccgag gccgccggcg acggccaccg
     7981 agatctcgtc gacgttgacc gaatccagga tgtaggtcga cacgccgccg ccgttgaagg
     8041 gcgccgcgat cgtcatgcca ttgatcgcca tccgcccttc gttgatcgga ccgccgcgcg
     8101 ccgcgaaaaa ggtcatcgtc ggcgatgcag cgagggcgcc gtcgttgacg accagaccgg
     8161 ggacggcatt cagcagggtc ccgacggagc ggttccccgg gatagccgag acgacgtcgg
     8221 cgctgatcac ggtctcgcgc tgcgtcgtct ggaggtcgac caccggagac tcaccggtga
     8281 ccgtgatcgt ctccgcgaga gagccgacac gcatatcgac cggaatcgtc agcgttgcgg
     8341 tgccgctcag ttcgatgccc tctcgcttga cgacgatgaa gccggtcagc gtgaaggtca
     8401 gcgaatacgt gccgggattc agacccgtaa tgcggtactg cccggcgcca tcggtcaccg
     8461 ccgcgcgcac cttttcgatg agggcagggc tggacgcctc gacggttaca cccggcagca
     8521 ccgcgctcga ttggtctctg acggtgccgg tcagggtacc ttgtgcagat agtacagatg
     8581 gcagcaggac tgcggccaga atagccgcgg gaatcaagac gaccttcgac gctcggcgca
     8641 tagtggtcct cctcaagtac cgagtgagtt ccagacgaca gcgcggcccc ggccgacgga
     8701 ttggtgtcgg cattcaacga cttctgaatg cttttgtcaa cgaggtgtac gacgccggcg
     8761 gtccttcagg cgtgcggcgc ccagatcttc tcgcctggct tgtacggggc gcagggatcg
     8821 tagcgtcccg gcgtttccac gtatcgcatc gcctggcggt agccgatctc tgaggtgaaa
     8881 tagccgagca gcgccagctc cttcatcaac cggaagtagt ggacgggcgc atcgccgggc
     8941 ggcggcgcgg cgggcgctcg cgaggtcagc gccgcttcgc gcgcatcggt ctcggcctgc
     9001 tgctcggcgt cgagcctccg aagcagcgcg agccgctggt ccggccgcgc ctgcagaaac
     9061 ggcgcggtga actctcgtcg tgacgcctcg tccatctggc gcatgccgtc gcggaagatc
     9121 gcctgctggc ggtgcgtgta ggtgtcggtg accatgagcg ccatgaacgc gcccgtggcg
     9181 gcggcctttg cgccgggagt gcccgtctcg ggaaggatgg tctcggccac ttcatcgagg
     9241 aaggcgacgt cggcggccga gaacgtgccg acgccccggg ctgcggctgc gcgctgcgcg
     9301 gcgcccgcgg ctggagccgc ctccagcagc tgctctcccc caacgagcgc agcgccgccg
     9361 agcaggacgg tgacgcgttg gatcgcctcg cgtcgagtca ccatcacaag ttaccgcgct
     9421 tcagctcctc gacggcgtac gccgccgccc gggctgtcag cgccatgtat gtgagcgacg
     9481 ggttctgaca ggccgacgag gtcatgcagg caccgtcggt gacgaagacg ttcgggcagt
     9541 cccagacctg gttgtgcgcg ttcaacacgg acgtcttcgc gtcgcgcccc atccgcgcag
     9601 tccccatctc gtggatgccc atgccgggag cgtagtcgtt gtcgtagacc cggacgtccc
     9661 ggacacctgc cgcctcgagc atctcggcca tgtcgttcat catgtcgcgc cgcatcagcc
     9721 gctcgttctc gcccgtcgcg cagtcgatcg cgagcacggg cagcccccac ttgtccgtgc
     9781 gggcgtcgat ccagacgcgg ttgccgtggt tcggcagcat ctcgccgaac gcggtggcgc
     9841 cgatccgcca tgggcccggc gtggccatgg cgtccttgaa tgcgccgccg acgccgagct
     9901 ccgccactgc gcgctcccat ccctgacggc tggcgctgcc ctggtagccg aagccacgca
     9961 ggtactcgcg ccgctcgtcg cctggcaggt tgcggaaccg cgggatatag aaaccggtcg
    10021 gccggcgccc gtacacgtag ctgtcctcgt gcccctcgat ctcgccgttg gcgccgcagc
    10081 ggaaatggtg atccatcagg ttgtggccca gctcgccgga gctgctgccg agcccgcccg
    10141 gccagacgtc ggtcgcggac cgcatgagga tccaggtcga gttgagcgca gaggcgcaca
    10201 ggaagatcac gggcgccgag tactccgtga cctg
//
LOCUS       DBNGSH010000197         2608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4260156, whole genome shotgun sequence.
ACCESSION   DBNGSH010000197 DBNGSH010000000
VERSION     DBNGSH010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4260156"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC16CY_04480"
     CDS             complement(<1..815)
                     /locus_tag="AC16CY_04480"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3353067.1"
                     /translation="MNGLNYHHLLYFWTVAREGGVSRAAEKLRLSQPTVSAQIKLLET
                     AFGERLFQRQGRTLVLTDVGRVVDRYASEIFNVGSELLETLKGRPSGRAPRLTVGVAN
                     AVAKLVVYRLLRPATEGDEAVQITCSEGDPDQLVAQLATHAIDVVISDTPAPPHIRVK
                     VFNHLLGESGTTFFAGKTLARRLRRQFPRSLDGAPMLLPTINTSLRRTLEQWFETEGL
                     HPVLAGEFEDPALLTTFGQSGRAVFPAPTAIEVEVQKSHGVAVVGRTAAVRER"
     gene            921..>2608
                     /locus_tag="AC16CY_04485"
     CDS             921..>2608
                     /locus_tag="AC16CY_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3353068.1"
                     /translation="MSGEQIQNPHTLSVDEALARAGSGLTGLAQAEAAHRFQIYGPNE
                     LQATTRESAWRTLAAQFQNVLILILLSATVISGLLGHTLEAIVITVIVLFAVLLGFIQ
                     EHRAGRALEALRRMAAPTAHVIREGLESVVPARDLVPGDLVQLRAGDRIPADARVVQA
                     VNLAIDEAALTGESAPVEKTIDPLADAALSIGDRTNMTYAGTLVTRGRGQAMVVTTGM
                     STEFGRIARMVETVEAGRTPLQANLDRLGATLGKAALAVVALVVVIGLSRGLPVIQMF
                     MFGIALAVAVVPEALPAVVTISLAIGVRRMVRRNALVRRLPIVETLGGTSVICSDKTG
                     TLTKNEMTVRQVFTEDRLFELSGAGYDPAGQLSENGRIVDPPPSVTALLQAGALASDA
                     RLVERDHRWQVEGDPTEGALVVAATKVGLSPAGLNEREPRVGEIPFTSERRRMTTLHT
                     TSAGVVAYLKGAAEDVLQACTRQAHSQQEIQLTDAGRERIRSVELRMAGEGLRVLAVA
                     RKSGASIENAEREMTLLGLVAMMDPPRPEARHAVQVCETAGIRPVMITGDHPLTA"
BASE COUNT          402 a          894 c          899 g          413 t
ORIGIN      
        1 taccgctccc gaaccgccgc ggtcctcccg acgaccgcca cgccgtggct cttctgtact
       61 tcgacctcga tcgcggtggg cgccgggaac accgcccggc cagactggcc gaacgtcgtg
      121 agcagcgcgg ggtcctcgaa ctccccggcg agcacgggat gaaggccctc ggtctcgaac
      181 cactgctcga gcgtccgccg cagcgacgtg ttgatcgtcg gcaggagcat gggcgcgccg
      241 tcgagtgagc ggggaaactg gcgccgcagc cgccgggcca gcgtcttgcc cgcgaagaac
      301 gtggtgcccg attcgccgag caggtggttg aacaccttga cccggatgtg agggggcgcg
      361 ggcgtgtcgc tgatcacgac gtcgatggcg tgcgtcgcga gctgggcgac gagctggtcg
      421 gggtcgcctt cgctgcaggt gatctgcacg gcctcgtcgc cctcggtcgc cggccgcagc
      481 aggcggtaca cgacgagctt ggcgacggca ttcgccacgc cgaccgtcaa gcgcggtgcg
      541 cgtcccgacg gccgcccctt gagcgtctcg aggagctcgc tgcccacgtt gaagatctcc
      601 gacgcgtacc ggtcgacgac gcggccgacg tcggtcagca cgagggtgcg cccctgacgt
      661 tgaaacagcc gctccccgaa cgcggtctcc aggagcttga tctgcgcgct gaccgtcggc
      721 tgcgacagcc gcagcttctc ggcggccctc gagacgccgc cttcgcgcgc cacggtccag
      781 aagtacagca ggtggtggta gttgagcccg ttcatgacgg catcgtcctc tcgctcaaca
      841 tagcaaaaac ctcacgattc gctcataact ttctatttgc gtatgcgggt ggccatcgcc
      901 tatcgtcgag ttcgcaggtg atgagcggcg aacagattca gaatccacac acgctgtcgg
      961 tggacgaggc gctcgcgcgc gcggggagcg ggctgaccgg cctcgcgcag gctgaggcgg
     1021 cccatcgatt tcagatctac ggcccgaacg agctgcaggc cacgacccgt gagtccgcct
     1081 ggcgcaccct cgccgcccag ttccagaacg tcctgatcct gatcctgctg agcgcgaccg
     1141 tcatctcggg gctgctcggg cacacgctcg aggcaatcgt catcacggtg atcgtcctgt
     1201 tcgcggtgct gcttggcttc atccaggagc atcgtgccgg acgcgcactc gaggcgctga
     1261 ggaggatggc ggcgccgacg gcccacgtga ttcgcgaggg actcgaaagc gtcgtcccgg
     1321 cccgggacct cgtaccgggc gatctcgtgc agctgcgcgc gggcgatcgc atcccggcgg
     1381 atgcgcgtgt cgttcaggcg gtcaatctgg cgatcgacga ggccgccctg acaggggaat
     1441 cggcgccggt cgagaagacg atcgaccctc tcgccgatgc ggcgctgtcg atcggcgacc
     1501 ggaccaacat gacctacgcc ggcacgctgg tgacccgcgg ccgcggccag gcgatggtcg
     1561 tcacgaccgg catgtccacc gagttcggcc gcatcgctcg catggtggag acggtcgaag
     1621 ccggccgcac tcccctgcag gccaacctcg atcggctcgg cgccacgctc ggcaaagccg
     1681 cgctggccgt cgtcgcgctc gtcgtcgtga tcggcctcag tcgcggattg ccggtcatcc
     1741 agatgttcat gttcggcatc gccctggcgg tcgccgtggt acccgaggcg cttcccgccg
     1801 tcgttacgat ctccctggcg atcggcgtgc ggaggatggt caggcgcaat gcgctcgtcc
     1861 gccgcctgcc gatcgtcgag acgctgggcg gcacgtcggt catctgttcc gacaagacgg
     1921 gcacgctgac caagaacgag atgaccgtcc ggcaggtttt taccgaggat cgcctcttcg
     1981 aactgtcggg cgccgggtac gaccccgcgg gacagctctc cgagaacggc cgaatcgtgg
     2041 atccgccgcc ttcggtgacc gcgctgctgc aggccggcgc gctcgcgtcg gatgcgcggc
     2101 ttgtcgagcg ggatcaccgg tggcaggtcg agggcgaccc gactgaaggc gcgctcgtcg
     2161 tggccgccac gaaggtcggg ttgagcccgg ccggtctcaa cgagcgcgaa ccacgggttg
     2221 gcgaaatccc cttcacctcg gaacggcgtc gaatgaccac gctccacacg acgtcggcgg
     2281 gcgtcgtggc ctatctcaag ggggccgcgg aagatgtgct gcaggcatgc acgcgacagg
     2341 cgcactcgca gcaggagatc cagctgaccg acgccggccg cgagcgcatc cgttccgtcg
     2401 agctgcggat ggcgggcgag gggctgcgcg tgctcgccgt cgcgaggaag tcgggcgcct
     2461 ccatcgagaa tgccgagcga gagatgaccc tgctcggcct ggtcgcgatg atggatcccc
     2521 cgcggcccga agcgcggcac gccgtgcagg tctgcgagac cgccggcatc cgccccgtca
     2581 tgatcaccgg cgatcatccg ctgacggc
//
LOCUS       DBNGSH010000198         2053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1543817, whole genome shotgun sequence.
ACCESSION   DBNGSH010000198 DBNGSH010000000
VERSION     DBNGSH010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1543817"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2053)
                     /locus_tag="AC16CY_04490"
     CDS             complement(<1..>2053)
                     /locus_tag="AC16CY_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR3353069.1"
                     /translation="LASAQDKSKAVKNYVKAIGKGLKKVMSKMGISTYMSYTGSQIFE
                     ALGLARSLVDKYFTGTASNVEGIDLFDLAEEAIRIHRAAFDERDPVLAHMLDAGGEYA
                     WRVRGEDHTWTPDAIAKLQHASRANSYATYKEYAAIINDQSRRHMTFRGLFEFRFGLR
                     APVPIEEVESAAEIVKRFSTGAMSLGSISTEAHTTLAIAMNRIGGKSNTGEGGEDRRR
                     YAPVKAGESLQSRLGKSRVEVDIPLREGDSLKSKIKQVASGRFGVTAEYLASAEMLQI
                     KIAQGAKPGEGGQLPGRKVSEYIAELRYSTPGVELISPPPHHDIYSIEDLAQLIHDLK
                     NSNPNAAISVKLVSEVGVGTVAAGVSKGKSDHVTIAGHDGGTGASPWSSIKHAGTPWE
                     LGLAETQQTLVLNRLRGRIAVQVDGQMKTGRDVVIGAILGADEFGFATAPLVVEGCIM
                     MRKCHLNTCPVGVATQDPVLRRKFEGKPEHVVNYFFHVAEEARELMAQLGVRSFNELI
                     GRTEFLDMRKGVEHWKAKGLDFSRIFAMPKMPADVSRFHNEVQSHGLEKALDNRLIEL
                     ARPALERGEKVAIETPIRNINRTVGTMLSHEIAKRYGHEGLPDDTIHIRFAGSAGQSF
                     GAFLAKGVTLDLVGDTNDYCGKGLSGGRISVQPSPKFRGEPTENIITGNVVLYGAIAG
                     EA"
BASE COUNT          326 a          674 c          652 g          401 t
ORIGIN      
        1 gcttcgccgg cgatcgcgcc gtagagcacg acgttgccgg tgatgatgtt ctccgtcggc
       61 tcgccgcgga acttcggcga gggctgcact gagatccgac cgccggagag ccccttgcca
      121 cagtaatcgt tggtgtcgcc caccaggtcg agcgtcacgc ccttcgcgag gaaggcgccg
      181 aagctctggc cggccgagcc ggcgaagcga atgtggatcg tgtcgtcggg cagcccctcg
      241 tggccgtagc gcttcgcgat ctcgtgcgac agcatcgtgc cgacggtgcg attgatgttc
      301 cgtatgggcg tctcgatcgc tactttctcg ccgcgctcga gcgccggccg cgccagctcg
      361 atcaggcggt tgtcgagcgc cttctcaagc ccatggctct gaacttcatt atggaaacgg
      421 gacacgtccg caggcatctt cggcatcgca aaaatgcgcg agaagtcgag ccccttcgcc
      481 ttccagtgct cgaccccctt cctcatatcc aggaattccg tacgaccgat cagctcattg
      541 aaggagcgca caccgagctg cgccatgagc tcgcgcgcct cctcggcgac gtggaagaaa
      601 taattcacca cgtgctcggg cttgccttcg aacttacgcc gcagcaccgg gtcctgggtc
      661 gccacgccga ccgggcaggt gttcaggtgg cacttgcgca tcatgatgca gccctcgacg
      721 accagcgggg cggtggcgaa gccgaactcg tcggcgccca ggatcgcgcc gatcaccacg
      781 tcgcgcccgg tcttcatctg gccgtcgacc tgcacggcaa tgcggcctct caatctgttc
      841 aagaccaggg tttgttgagt ctcggcaagg cccaattccc acggcgtgcc ggcatgcttg
      901 atcgaggacc acggagacgc tccggtcccg ccgtcgtgcc cggcgatcgt gacatggtcg
      961 gacttgccct tcgatacgcc tgcggcgacc gtgccgacgc ccacctcgga cacaagcttc
     1021 accgagatgg cggcgttcgg gttcgagttc ttgagatcgt ggatgagctg cgccaggtcc
     1081 tcgatcgagt agatgtcgtg gtgcggtggc ggcgagatca gctcgacccc cggagtcgag
     1141 tagcggagct cggcgatgta ctccgacacc ttgcggcctg gaagctggcc gccttcgcct
     1201 ggcttggcgc cctgcgcaat cttgatctgc agcatctcgg cgctcgccag gtactcggcg
     1261 gtgacgccaa agcggcccga ggcgacttgc ttgatcttcg acttgagcga atcgccctct
     1321 cgcaacggga tgtccacctc aacgcggctc tttcccaacc tgctttgcag ggattcgccc
     1381 gccttcacgg gtgcataacg ccggcggtcc tcgccgccct ctccggtgtt cgatttcccg
     1441 ccgatgcggt tcatggcgat cgcgagcgtt gtatgcgcct ccgtcgagat cgagccgagc
     1501 gacatcgccc cggtggagaa gcgcttgacg atctcggccg cggactcgac ttcctcgatc
     1561 ggcaccggcg cgcgcagacc gaagcggaac tcgaacagcc cgcggaaggt catgtggcgc
     1621 cgcgactggt cgttgatgat cgccgcgtat tctttgtacg tggcgtagga gttggcgcgg
     1681 gaggcgtgct gcagcttggc aatcgcgtcg ggggtccagg tgtgatcctc gccgcgtacc
     1741 cgccaggcgt actcgccgcc cgcgtccagc atgtgggcga gtacgggatc gcgctcgtcg
     1801 aacgccgcgc ggtggatccg gatcgcttct tccgcgaggt cgaaaaggtc gatgccctcg
     1861 acgttcgaag cggtgccggt gaagtacttg tcgacgagcg acctcgccag gccgagcgcc
     1921 tcgaagatct gggagccggt gtaggacatg taggtcgaga tgcccatctt ggacatcact
     1981 ttcttcagtc ccttgccgat cgccttgacg tagttcttga ccgccttgct cttgtcctgc
     2041 gccgacgcaa gta
//
LOCUS       DBNGSH010000199         3104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3920867, whole genome shotgun sequence.
ACCESSION   DBNGSH010000199 DBNGSH010000000
VERSION     DBNGSH010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3920867"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1211
                     /gene="sucC"
                     /locus_tag="AC16CY_04495"
     CDS             48..1211
                     /gene="sucC"
                     /locus_tag="AC16CY_04495"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR3353070.1"
                     /translation="MKIHEYQAKSVLARFGVAVPRGEVAFTPAEAAEIARRLGGGPAV
                     VKAQIHAGGRGKGGGVKLAKSPEEVEQLATQMLGMTLVTHQTGPDGRKVGRVLVEEGL
                     QIDRELYLSIVIDRAAGRPVVICSAAGGMDIEEVAAREPQKILREHIDPATGLVPFQS
                     RKLAFGMGLGSGPAAKLAKLLEAVFKAFIDTDASMIEINPLILTKNGDLLALDAKVTF
                     DDNALYRHADFRDLRDLSEEDPLEVEASTFSLNYIRLDGNIGCMVNGAGLAMATMDII
                     KLAGGEPANFLDVGGGANADQIKNAFRILMADRNVKAVLINIFGGILRCDVLAQGLIA
                     AVTELHVPVPIVIRMEGTNVEEGKRLLRESGLNFTTADSMGEAATKVVGLARG"
     gene            1213..2085
                     /gene="sucD"
                     /locus_tag="AC16CY_04500"
     CDS             1213..2085
                     /gene="sucD"
                     /locus_tag="AC16CY_04500"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3353071.1"
                     /translation="MAILLDRNTRLIVQGLTGREGTFHAKAAAAYGTRVVGGVTPGKG
                     GTTHEGWPIFNGVLDAVRDTGANASVIFVPPQFAADAIMEAADAGLPLVVCITEGIPT
                     IDMMKAMAFLKGKTTRLVGPNCPGVITAGQAKAGIIPGHICREGRIGIVSKSGTLTYE
                     AIHQLSRQGMGQTTCIGIGGDPLIGTSFIDALQLFKADADTDAVVMIGEIGGTAEQDA
                     AAYIAREFRKPVVGFIAGQTAPPGRRMGHAGAIIAGGQGTAAEKMDALSRAGVHVVKS
                     PADMGAKMREALGR"
     gene            2114..2527
                     /gene="ndk"
                     /locus_tag="AC16CY_04505"
     CDS             2114..2527
                     /gene="ndk"
                     /locus_tag="AC16CY_04505"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR3353072.1"
                     /translation="MERTLAIIKPDAVKSRRAGQILARIEAAGFTVRAMRLQHLTKRE
                     AEGFYAVHRERPFFGSLTDFMSSGPCILLALEAPDAIKKWRATMGATDPGKAEAGTIR
                     KEFGTSIEHNATHGSDAPDTAAFELGYFFRGLETI"
     gene            2534..2728
                     /locus_tag="AC16CY_04510"
     CDS             2534..2728
                     /locus_tag="AC16CY_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR3353073.1"
                     /translation="MGRVLIIVGLLIAAAGVLISLGVPLGRLPGDFTIRRGNFTFYLP
                     LASSLLASIVLTLVMMLFRR"
     gene            2761..>3104
                     /locus_tag="AC16CY_04515"
     CDS             2761..>3104
                     /locus_tag="AC16CY_04515"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR3353074.1"
                     /translation="MPSIKADRWIQRMALEHGMIEPFEGRQVRKGVISYGLSSYGYDI
                     RVADEFKVFTNINNTVVDPKNFDDRSFVDMKADVCIIPPNSFALAKTVEYFRIPRDVL
                     TVCVGKSTYARCG"
BASE COUNT          574 a          982 c         1057 g          491 t
ORIGIN      
        1 gatgaggggc cgttgacgtc acgaggccgg aagctccgta tccccctgtg aaaattcacg
       61 agtatcaagc caagtcggtc ctcgcgcgtt ttggcgtcgc cgtgccgcgc ggagaggtgg
      121 cgttcacgcc ggcggaagcg gccgagatcg cgcgccgcct cggcggcggc ccggccgtcg
      181 tcaaggcgca gatccacgcc ggcggacgag gcaagggcgg aggcgtcaag ctcgcgaagt
      241 cgcccgagga agtcgagcag ctcgccaccc agatgctcgg gatgacgctc gtgacgcatc
      301 agaccggccc cgacggacgc aaggtcggcc gggtgctcgt cgaagaaggc ctgcagatcg
      361 accgggagct gtacctgagc atcgtcatcg atcgggcggc gggccggccc gtcgtcatct
      421 gcagcgccgc aggcggaatg gacatcgaag aggtggccgc gcgggagccg cagaagatcc
      481 ttcgcgagca catcgatcca gccacgggcc tcgtcccgtt ccagtcgcgg aagctggcgt
      541 tcggcatggg cctcggctcg ggacccgccg ccaagctggc gaagctcctc gaggcggtgt
      601 tcaaggcgtt catcgacacc gatgcctcga tgatcgagat caatccgctc atcctgacga
      661 agaacggcga tctgctggcc ctcgacgcca aggtgacctt cgacgacaac gcgctctaca
      721 ggcacgccga ctttcgcgac ctcagggact tgtcggagga ggatccgctc gaggtcgaag
      781 catcgacctt ctcgctcaac tacatccgcc tcgacggcaa catcgggtgc atggtcaacg
      841 gagccgggct ggccatggcc acgatggaca tcatcaagct cgcgggtgga gagccggcga
      901 acttcctgga cgtcggcggc ggcgcaaacg ccgatcagat caagaacgcg ttccggatcc
      961 tgatggccga ccggaacgtc aaggccgtcc tgatcaacat cttcggtggc atcctgcgct
     1021 gcgacgtgct ggcacagggg ctcatcgcgg cggtgaccga gctgcacgtg cccgtgccga
     1081 tcgtgatccg catggaaggc acgaacgtgg aggagggcaa gcgcctgctc cgcgagagcg
     1141 gcttgaactt cacgacggct gattccatgg gcgaggcggc gacgaaggtc gtgggcctgg
     1201 ccaggggatg acgtggcgat tcttctcgat cgaaacacgc ggctgatcgt tcagggcctg
     1261 accgggcgcg aggggacgtt tcacgcaaag gcggcggctg cgtacggcac acgggtcgtg
     1321 ggaggcgtga caccggggaa aggtggcacg acgcacgagg gctggccgat cttcaacggg
     1381 gtcctcgacg ccgtccgcga caccggcgcc aacgccagcg tcatcttcgt gccgccgcag
     1441 tttgccgccg acgcgatcat ggaggctgcc gacgccggcc ttccgctcgt cgtctgcatt
     1501 acggaaggca ttcccacgat cgatatgatg aaggcgatgg ccttcctgaa ggggaagacg
     1561 acgcgcctcg tcgggccgaa ctgtcccggc gtgattaccg cggggcaggc gaaggccgga
     1621 atcattcccg gccatatctg ccgcgagggg cgaatcggca tcgtgtccaa gagcggaacg
     1681 ctgacctacg aggcgatcca ccagttgtcc aggcagggga tgggacagac tacctgcatc
     1741 ggcatcggcg gtgacccgct gatcggcacg agcttcatcg acgcgctgca gttgttcaag
     1801 gccgacgccg acaccgacgc cgtcgtcatg atcggagaga tcggcgggac cgcggagcag
     1861 gacgccgcgg cctatatcgc ccgcgagttc cgcaagcccg tcgtcgggtt catcgcgggc
     1921 cagacggcgc caccggggcg gcgcatggga cacgccggcg ccatcatcgc ggggggccag
     1981 gggacggcgg ccgagaagat ggacgcgctg tccagggcgg gcgtccacgt ggtgaagagt
     2041 ccggcggaca tgggcgcgaa gatgcgcgaa gcgctgggac gctaggcgag taggcgagca
     2101 ggggactgag gacatggaac gaacactggc aatcatcaag cccgacgccg tcaagagccg
     2161 gcgcgcgggc cagatcctgg cccgcatcga ggcagccggg ttcaccgtgc gcgccatgcg
     2221 cctgcagcac ctgaccaaga gggaggcgga agggttctac gcggttcacc gcgagcgccc
     2281 cttcttcgga tcgctgaccg atttcatgtc ctccggcccg tgcatcctgc tcgccctcga
     2341 ggcgcccgat gcgatcaaga agtggcgtgc cacgatggga gccaccgatc cggggaaggc
     2401 cgaggccgga accatccgca aggagtttgg aacctcgatc gagcacaacg cgacgcacgg
     2461 atcggacgcg cccgacacgg cggccttcga gctggggtac ttctttcgcg gactcgaaac
     2521 catctagcaa ggcatgggtc gagtgctcat catcgtcggc ctgttgattg ccgccgccgg
     2581 cgtgttgatc tcgctcggcg tcccgcttgg acggctgccg ggcgatttca ccattcgacg
     2641 aggcaacttc acgttctatc tcccgctggc ctcgtcgctc ctggccagca tcgtcctgac
     2701 gctcgtcatg atgttgttca gacggtagcc cgtagcctgt agcccggaga gacagactca
     2761 atgccttcga tcaaagccga cagatggatt cagcgaatgg ccctcgagca cgggatgatc
     2821 gagccgttcg agggccggca ggtccggaag ggcgtcatct cctacgggtt gtcctcatac
     2881 ggctacgaca tcagggtcgc cgacgagttc aaggtcttca ccaacatcaa caacacggtc
     2941 gtggacccga agaacttcga cgaccggtcg ttcgtggaca tgaaggccga cgtctgcatc
     3001 attccgccca actccttcgc gctggccaag acggtggagt acttccggat tccgcgcgac
     3061 gtgctcaccg tgtgcgtggg caaatcgacg tatgcccgtt gcgg
//
LOCUS       DBNGSH010000200         2845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1883623, whole genome shotgun sequence.
ACCESSION   DBNGSH010000200 DBNGSH010000000
VERSION     DBNGSH010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1883623"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="AC16CY_04520"
     CDS             complement(<1..572)
                     /locus_tag="AC16CY_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353075.1"
                     /translation="MTKVDRARVLWLMLVVVLLAAAPAAAQPGRPQIPPDLAGEWRLE
                     NNEEDTTAQPPLGNYLGIPFNDAGRQRSDTTAESLWGTPEYQCRPHSAPHQWRGRGGA
                     RILKEQDPLTRNVAVYHIQFMRSLDRPVFMDGRPHPPAWAPHSWTGFSTGEWIGNTLK
                     ITTTHLKDGYLKRGGPQTSDMYNMTEFLTR"
     gene            complement(658..1392)
                     /locus_tag="AC16CY_04525"
     CDS             complement(658..1392)
                     /locus_tag="AC16CY_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HIR3353076.1"
                     /translation="MSRTSAQSSPVGNVKALVFDVFGTVVDWRNGVARDAERILEPLG
                     HKIDWLAFADAWRALYQPSMEEVRAGRQPFVKLDVLHRRMLDQIRPRFGLEKLRDAVA
                     DDLNLAWHRLDAWPDVAPGFARLSRKFVLAPCSNGNIALMVDIARRNNIPWDAILGSE
                     IARDYKPKPRVYLAAAEALNLSPGEVMMVAAHSDDLRNAASNGLRTGHVGRPGENGPG
                     TGERAPRVPVDVAARDFNDFADKLGA"
     gene            complement(1514..2350)
                     /locus_tag="AC16CY_04530"
     CDS             complement(1514..2350)
                     /locus_tag="AC16CY_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353077.1"
                     /translation="MNRHLRPSAVLAVAVMTAVVCQAQEPTLDEVVDRLGRYISDYET
                     KIVQLAAEEEYEQRIKRRSGYGGEVVARRKLTSTFLFARLPTGQAWFGVRDVVRVDGK
                     PVSGRGRSLEQLLQEGTLDALAEANRVVRENAKYNIGAVYRTVNLPLQAIELLRAEHR
                     RRFEFTSVGRARMAGSRVWQIAYAERVRPSLINDGFGGDRLTNGHVWVDPTTGAVHKT
                     EISIEKVNLITVEFRHNDRFQMLLPSSMEEVYELAIEVVNGRANYSDYRRFQTSARIV
                     DK"
     gene            complement(2360..>2845)
                     /locus_tag="AC16CY_04535"
     CDS             complement(2360..>2845)
                     /locus_tag="AC16CY_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3353078.1"
                     /translation="TNRFPIPMPVFHDGMIYMSRGYRSSPYMAIRPGGRGDVSKTHVV
                     WESATGAPYISSLVYDAGLIYMATDVGGVTVVDAKTGQRLSQQRVEGVFSASPVAADG
                     KIYFVSESGETIVMQSGSSPTVLARNDVGERAIASPAISNGQIFIRTDDHLFCIGTRA
                     G"
BASE COUNT          463 a          943 c          931 g          508 t
ORIGIN      
        1 tgacgcgtga ggaactccgt catgttgtac atgtcgctcg tctggggacc gccgcgcttg
       61 agatacccgt ccttgagatg cgtggtcgtg atcttgagcg tgttgccgat ccactcgccg
      121 gtcgagaagc ccgtccagct gtgcggcgcc caggccggcg gatgcgggcg tccgtccatg
      181 aacacgggac ggtcgagcga tcgcatgaac tggatgtggt agacggccac gttgcgcgtc
      241 aacggatcct gttccttcag gatgcgcgcg ccgccgcggc cgcgccactg gtgcggggcc
      301 gagtgcggcc ggcactggta ttccggcgtg ccccagagcg attcggctgt cgtgtcggaa
      361 cgctggcgcc ccgcgtcgtt gaacggaatg ccaagatagt taccgagcgg cggttgggcc
      421 gtcgtgtctt cctcgttgtt ctcgaggcgc cactccccgg ccagatccgg cggtatctgc
      481 ggccggcccg gctgcgcggc ggcgggcgcg gcggccagga gcaccacaac gagcatcaac
      541 cagagtaccc gcgcgcgatc gaccttcgtc atgtcctacc ccacgaagaa gataccgcat
      601 cttgccggtc gccggtagcc aacagccggc gtgggtcccg tattcgtcgt ttgcaggcta
      661 cgcgcccagc ttgtccgcaa agtcgttgaa atcgcgcgcg gccacgtcga cgggcacgcg
      721 tggcgcgcgt tctcctgtgc cgggtccgtt ctcgccaggc cggccgacgt gacccgtgcg
      781 caagccgttc gaagcggcgt tgcgcaagtc gtcgctgtgg gcggcgacca tcatgacctc
      841 tccgggcgag agattcaggg cttccgccgc cgcgagatac acgcgcggct ttggcttgta
      901 gtcgcgggcg atctccgagc cgagaatcgc gtcccacgga atgttgttcc gccgcgcgat
      961 atcgaccatc aacgcaatgt tgccgttcga acacggtgcg agcacgaact tgcggctcag
     1021 gcgcgcgaac cccggcgcca cgtcgggcca ggcgtcgagg cggtgccatg cgaggttcag
     1081 atcgtcggcg actgcgtccc ggagtttctc gaggccgaaa cgcgggcgga tctgatcgag
     1141 catgcggcgg tgcaggacat cgagcttgac gaatggctga cggcccgcgc gaacctcctc
     1201 catgctcggc tgatagagcg cacgccaggc gtcggcgaag gccagccaat cgatcttgtg
     1261 gccgagcggc tcgagaatcc gctccgcgtc gcgcgcaacg ccgttgcgcc agtcgacgac
     1321 cgtgccgaac acgtcgaaga cgagcgcctt gacgttgccg accggcgacg attgagcgga
     1381 agttcgagag atgtcgcctg cggcgagtgc ggccgcgaca gcgcctgcgc cgcgaagaac
     1441 gccacggcga gagatggttg tcacttctac cttcctcggg gtacgcctgc cacccccggc
     1501 tgccagctgg cggctactta tcgactatcc gcgcactggt ctggaacctc cggtagtcgc
     1561 tgtagttggc gcggccgttg acgacttcga tggcgagttc gtagacctct tccatcgacg
     1621 aggggagaag catctggaac cgatcgttgt gccggaactc gacggtgatc aggttcacct
     1681 tctcaattga gatctccgtc ttgtggacgg ccccggttgt cgggtcgacc cacacatgtc
     1741 cgttcgtcag ccggtcgccg ccgaatccgt cgttgataag cgacggccgc acccgttccg
     1801 cgtacgcgat ctgccacacg cgtgaaccgg ccatgcgcgc gcggcccacg gaggtgaact
     1861 cgaatcgacg gcggtgctcg gcacgaagca gctcgatcgc ttgaagcggc agattgactg
     1921 ttctgtagac tgcgccgatg ttgtacttgg cgttctcgcg cacgacgcgg ttcgcttccg
     1981 cgagcgcatc gagcgtaccc tcctggagca actgctcgag cgagcggcca cgcccgctta
     2041 cgggctttcc gtccaccctg accacgtcgc gcactccgaa ccacgcctgg cctgtcggga
     2101 gcctggcaaa gagaaacgtg gacgtcagct tccgccgcgc gacgacctca cccccgtacc
     2161 ccgatcgtct cttgatccgc tgttcgtact cttcctcggc cgccagctga acgatcttgg
     2221 tttcgtagtc gctgatgtag cggccgaggc gatcgaccac ctcgtcgagc gtcggctcct
     2281 gggcctgaca cacgacggcc gtcatcaccg cgacagccag gactgcggag ggccgaaggt
     2341 gtcgattcat aagcgggcct cagcccgcac gggtgccgat gcagaacaga tggtcgtcgg
     2401 tgcggatgaa gatctgtccg ttcgagatcg cgggcgaggc aatggcccgt tcgccgacgt
     2461 cgtttcgcgc gaggaccgtg ggcgacgagc cggactgcat cacgatcgtc tcccccgatt
     2521 cgctgacaaa gtagatcttg ccgtcggccg ccaccggcga cgccgagaac accccttcga
     2581 cgcgctgctg cgacagccgc tgcccggtct tggcgtcaac cacggtcacg ccgccgacgt
     2641 cggtggccat gtagatcagg cccgcgtcgt acacgagcga cgagatatag ggcgcccccg
     2701 tcgccgactc ccacacgacg tgcgtcttcg acacgtcgcc tcgtccgccg ggacggatcg
     2761 ccatgtaggg gctgctgcga tagccgcggc tcatgtagat catgccgtcg tgaaacaccg
     2821 gcatcgggat cggaaaccgg ttggt
//
LOCUS       DBNGSH010000201         2872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_864844, whole genome shotgun sequence.
ACCESSION   DBNGSH010000201 DBNGSH010000000
VERSION     DBNGSH010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_864844"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="AC16CY_04540"
     CDS             <1..1344
                     /locus_tag="AC16CY_04540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3353079.1"
                     /translation="YGTMAVDWEQRIDFDRLRRERLARIKGLLAKSEMGSVLCFDMNN
                     VRYITATHIGTWAQDKISRFTLLAQGAEPILWDFGSAAKHHRLNCPWLGERSRAGIPL
                     MRGAMSPEMGRSEDVARKIRVELEARGLHKEPVGVDVVELPVLFALQKEGLTIVDGQQ
                     LMSDAREIKTQDEISLLAQSAGMVDAAYFELYRAMKPGMKESEAVALVAKILYEMGSE
                     YVEGVNAISGERCSPHPHVFSDRVLRPGDPVYYDILHSYMGYRTCYYRCFAISSASRA
                     LVDAYKRCRDYLDASIELIRPGRTTSEVAAVWPKATEFGFPDEESAFALQMGHGIGLA
                     IWEKPMISRLVSLDHPHEIKPGMVFALETFWPSTDGWSAARIEEEIVVTETGHEVITR
                     FPAEELLIAGAPFVTVGGTLPTLREPEPPPNVQVKELIAASARAEVGRTDKVGAR"
     gene            1344..2582
                     /locus_tag="AC16CY_04545"
     CDS             1344..2582
                     /locus_tag="AC16CY_04545"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3353080.1"
                     /translation="MAVSVVMPALEMAQETGKLVSWKKKEGEPVRKGEMLLEIETDKA
                     VVEIEAGGDGVLAGVSAKEGDVVPVGQTIAWLLKPGEAVPAGGPQTQSGRKMESAAAS
                     AAPAAVAAPVAPPSAAGPRISPKARRLAQEHGIDITQLRGSGSGGEILADDILKAAAA
                     WSSRSSGPAAAFAPGASVPGKTETGDPAAVSSIGRIMAERTTQSWTTVPHFFVARDVD
                     ATALTAARERLIPAIEKSHGVKVTHTDLLVAAVARALRQHPRMNGSWANNTVALNSDV
                     NVALAMAVENAVVTAVIRNADQAPVGAIAKQRKELAERARSNKLQPADISGATFTISN
                     LGMFGVDAFTAIIVPPQAGILAVGAIADRVVAVEGMIGVRPMMTLTLSSDHRIVDGAR
                     AAQFLNDVVTSLSQPAQWLA"
     gene            2686..>2872
                     /locus_tag="AC16CY_04550"
     CDS             2686..>2872
                     /locus_tag="AC16CY_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3353081.1"
                     /translation="MNLGLKGRTALVTGASKGIGKAIARGLADEGVNLVLVARGQEDL
                     DRAAAEICKATGVRVVAV"
BASE COUNT          477 a          952 c         1009 g          434 t
ORIGIN      
        1 tacggcacga tggcggtgga ctgggagcag cggatcgact tcgatcggct gcggcgcgag
       61 cggctcgccc ggatcaaggg cctgctggcc aagtcggaga tgggctcggt gctctgcttc
      121 gacatgaaca acgttcgcta catcactgcc acgcacatcg gcacctgggc gcaggacaag
      181 atcagccgct tcacgctgct ggctcagggc gccgagccga tcctctggga cttcgggtcg
      241 gccgccaagc atcaccggct gaactgcccg tggctcggcg agcgctcgcg ggccggcatt
      301 ccgctgatgc gcggcgcgat gtcgcccgag atgggacgct cggaggacgt ggcccggaag
      361 attcgcgtcg agctcgaggc gcgcggactt cacaaggagc cggtgggtgt ggacgtggtg
      421 gagcttcccg tcctctttgc cctgcagaag gaaggcctga cgatcgtgga cggccagcag
      481 ctcatgtccg atgcgcgcga gatcaagacg caggacgaga tctcgctgct ggcgcagtcg
      541 gccggcatgg tcgatgccgc ctacttcgag ctgtaccggg cgatgaagcc gggcatgaag
      601 gagagcgagg ccgtcgcgct cgtggcgaag atcctctacg agatgggctc ggagtacgtc
      661 gaaggcgtca atgcgatctc tggcgagcgc tgcagcccgc atccgcacgt gttctccgac
      721 cgcgtgctgc ggccgggcga cccggtgtac tacgacattc tgcattccta catgggctac
      781 cggacctgct actaccggtg cttcgccatc agctccgcgt cgcgcgcgct cgttgacgct
      841 tacaagcgat gccgcgacta tctggacgcc tcgatcgagc tgatccgccc gggccgcacc
      901 acgtccgaag tggccgccgt gtggcccaag gccaccgagt tcggcttccc ggacgaggaa
      961 tcggccttcg cgctgcagat ggggcacggc atcggcctgg cgatctggga gaagccgatg
     1021 atcagccgcc tcgtctcgct cgatcatccg cacgagatca agccgggcat ggtgttcgcg
     1081 ctcgaaacgt tctggccctc gaccgacggc tggagcgccg cccgcatcga ggaagagatc
     1141 gtcgtcaccg agacgggcca cgaggtgatc acgcggttcc cggccgagga gttgctcatc
     1201 gccggcgcgc cgtttgtgac cgtcggcggg acgctgccga cgctgcgcga gcccgagccg
     1261 ccgcccaacg tgcaggtcaa ggaattgatc gcggcatcgg cgcgcgccga ggtcggccgg
     1321 accgacaagg tcggggcgcg ctgatggccg tcagcgtggt catgcctgcg ctcgaaatgg
     1381 cgcaggaaac cggcaagctg gtctcgtgga agaagaagga aggcgagccg gtccgcaagg
     1441 gcgagatgct gctcgagatc gagaccgaca aggccgtcgt cgagatcgag gcgggcggtg
     1501 acggcgtgct cgccggcgtc agcgcgaagg aaggcgacgt ggtgccggtc ggccagacga
     1561 tcgcgtggct gctcaagccg ggtgaagccg tgccggcggg cgggccccag acgcaaagcg
     1621 gccgcaagat ggaatcggcg gcggcgtcgg ccgctccagc ggcggtggcg gcccccgtcg
     1681 ctccaccctc ggcggcaggc ccgcgaatat cgccgaaggc gcgccgcctg gcgcaggagc
     1741 acggcatcga catcacccag cttcgcgggt ccggatcggg cggcgagatc ctcgccgacg
     1801 acattctcaa agccgcggct gcatggtcat cgcgatcgtc aggtccggct gccgctttcg
     1861 cgccaggcgc ttcggtgccc gggaaaaccg agaccggcga tcccgccgcg gtctcgtcca
     1921 tcggccgcat catggccgag cgcacgacgc aaagctggac gacggtcccc catttcttcg
     1981 tggcgcgcga cgtcgatgcc acggcgctca cggccgcgcg cgagcggctc attccggcca
     2041 tcgagaagtc gcacggcgtc aaggtgactc acaccgacct cctcgtcgcc gccgtggcgc
     2101 gcgccctgcg gcagcacccg cgcatgaacg ggagctgggc caacaacacg gtggcgctca
     2161 actccgacgt caacgtggcg ctggccatgg cggtcgagaa cgccgtcgtg acggccgtca
     2221 tccggaatgc ggaccaggcg cccgttggcg ccatcgcgaa acagcggaag gaactggccg
     2281 agcgcgcgcg atccaacaag cttcagccgg ccgacatctc cggagccacc ttcaccatca
     2341 gcaacctcgg catgttcggc gtcgatgcct ttaccgccat catcgtgccg ccgcaggcgg
     2401 gcatcctcgc cgtcggcgcg attgccgatc gggtggtggc ggtggagggc atgatcggcg
     2461 tgcgcccgat gatgacgctc acgctgtcgt cggaccatcg catcgttgac ggcgcccgcg
     2521 cggcgcaatt cctcaacgac gttgtgacgt ccctgagcca gcccgctcaa tggctggctt
     2581 gacgcggaac tacaggcggg tctaaaggcc cgcctctaca tgacgcggga tgtgactcgt
     2641 agcggcgggt cttcagaccc gccgtgatca ggaccgactg aaaacatgaa tctcgggctc
     2701 aagggccgta ccgctctggt gaccggcgcg agcaagggca tcggcaaggc gatcgcgcgg
     2761 gggctcgccg acgagggcgt caacctcgtg ctcgtcgcgc gcggccagga ggatctcgat
     2821 cgcgcggctg ccgagatctg caaggcgacc ggcgtgcgag tcgtggccgt gc
//
LOCUS       DBNGSH010000202         2884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2902607, whole genome shotgun sequence.
ACCESSION   DBNGSH010000202 DBNGSH010000000
VERSION     DBNGSH010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2902607"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..530)
                     /locus_tag="AC16CY_04555"
     CDS             complement(186..530)
                     /locus_tag="AC16CY_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3353082.1"
                     /translation="MQAQPFEILRLMLEHPRRVVTRDELRRRLWNDGTHVDFEHSLNA
                     AIRRLRMALGDRPTSVETVPRLGYRFIAPAQNPGGEPQTTRENALPVPRLVVDVCFTA
                     RVVYAISEPSTS"
     gene            complement(618..2081)
                     /locus_tag="AC16CY_04560"
     CDS             complement(618..2081)
                     /locus_tag="AC16CY_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HIR3353083.1"
                     /translation="MDLDIDACYRAFCTRDPRFDGRLFSGVKTTGVYCRPICPARTPK
                     RENMLFFPTAAAAQEAGFRPCLRCRPETVPELAAWRGTSNTVSRALSLIEHGALDEAG
                     VGELADRLGVGERQLRRLFREHVGASPIAVRQTRRVLLALQLIRETRLSIADVALASG
                     FGSIRRFNEVFQRLYRRSPTSLRRTSGTPSAGPQGEVSILLRYRPPYDWPAMLEFLRD
                     RAIPGVESVSDGRYARTIEIEGTHGVVIVEAAAGHALKAAIRFPRLSALPAVIARIRR
                     LFDLAADPHAIDAHLAEDSVLAPLVAARPGLRVPGAWDGFELVVRAVLGQQITVRAAA
                     GLAAKLVARYGEQLRDPSLAVDGLSHVFPRPECVALADPATLAMPMTRGRALTSLASA
                     VARNPGILCSSQEPAACVKRLRAFAGVGDWTAEYIAMRQLREPDAFPADDVGLQRAIA
                     RRDGRRPTSRELVGLAERWRPWRAYAAQHLWTSLARG"
     gene            complement(2360..>2884)
                     /locus_tag="AC16CY_04565"
     CDS             complement(2360..>2884)
                     /locus_tag="AC16CY_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353084.1"
                     /translation="LQGVNINDKHIEVISRQMMRWVRIEDVGDTEFLIDEQTDKFRFL
                     EENERCIQAGGRPATAQPLLLGITKASLSTDSFISAASFQETTRVLTEASISGKIDHL
                     RGLKENVTMGRLIPAGTGFEWYRHVRIPADEPPPPPVPPQPTEDELDLDREIEYAFDT
                     DEPMLGRPPAGDVE"
BASE COUNT          480 a          991 c          959 g          454 t
ORIGIN      
        1 cggctcgacc gcgcgccgac tgagtgtgcg agcctgagcg acggcctcag ctcttccact
       61 gtgtcgacag gcagcgaccg cctacccctg cgcacattgc tgtacgaggc gcggccacag
      121 ttcaccggtg gccggaccgg cgtcgccgct gttggaggca tctcaacggc tatccatcgg
      181 cacagtcaag atgtcgatgg ctcgctgatc gcatagacca cccgggctgt gaagcacacg
      241 tcaacgacga gacgcgggac tggcagggcg ttctcgcgtg tcgtctgtgg ctcgccgccg
      301 ggattctgcg caggggcgat gaagcggtag ccgagtcgag gcacggtctc aacggacgtc
      361 ggccggtcgc ctaacgccat acgcaagcgc cgtattgcgg cgttcaggct gtgctcgaaa
      421 tccacgtggg tgccgtcgtt ccacaggcgt cgccggagct cgtcgcgcgt gaccacacgt
      481 ctcgggtgtt cgagcatcag gcgcaggatc tcgaaaggct gcgcctgcag ccgaacgacc
      541 gtcgagccgt ttctgagctc ccagggtgca cgtcgagctc gaacgcgcca aagcgcagca
      601 gtcccatggt gtccatgtca acctcgggcc agggacgtcc agagatgctg cgccgcatac
      661 gcgcgccacg gacgccagcg ttcagcgagg ccgacgagct cccgcgacgt gggccgtctt
      721 ccatcgcgcc gcgcgatcgc tcgctgcaat ccgacgtcgt cggcaggaaa ggcgtcgggc
      781 tcgcgcaact gccgcatggc gatatattcc gccgtccagt cgccgacgcc ggcaaaggcg
      841 cgaaggcgct tcacgcacgc ggccggttcc tggctcgaac agaggatgcc cggattgcgg
      901 gcaacggcgg aggcgagcga cgtcagcgca cgcccgcgcg tcatcggcat ggcaagcgtc
      961 gcagggtcgg ccaacgcaac acactcgggc cggggaaaca catggctcaa gccgtccacg
     1021 gcgaggctgg gatctcgcag ctgttcgccg tacctggcaa cgagcttcgc ggcaaggccc
     1081 gccgccgccc tcacggtgat ctgctgtccg agcacggcgc gaacaacgag ctcgaagccg
     1141 tcccatgcgc cgggcacgcg aagccccggt cgcgccgcca ccagaggcgc gaggaccgag
     1201 tcctcagcca ggtgcgcgtc gatggcatga gggtcggcgg cgaggtcgaa caggcgtcga
     1261 atccgggcga tgacagccgg tagcgccgac aagcggggaa agcggatcgc cgctttgagc
     1321 gcgtgtcccg ccgcggcctc gacgatgacc acgccgtggg tgccttcgat ctcgatcgtg
     1381 cgtgcgtagc ggccgtctga caccgactcg actcccggaa tcgcacggtc tctcaagaac
     1441 tcgagcatgg ccggccagtc gtacggcggc cgatagcgaa gcaggatgct cacctctcct
     1501 tgaggtccag ctgacggtgt accgctcgtc cgtcgcagcg acgtcggcga gcgccggtag
     1561 agcctctgga agacctcgtt gaagcgccgg atgctgccga acccggacgc gagcgccacg
     1621 tcggcgatcg acagcctggt ttcgcgtatc agctggaggg ccagcagcac gcgccgcgtc
     1681 tgccggacgg caatgggcga cgcgccgacg tgttcacgga acaggcggcg caactgccgc
     1741 tcgcctacac caaggcggtc agccagctca ccgactccag cctcgtccag ggcgccgtgc
     1801 tcgatgagag acaacgctcg agagacggtg ttcgacgtgc cccgccaggc ggccagttcg
     1861 ggaaccgttt cgggcctgca ccgcaagcac ggccggaagc ccgcctcttg tgccgcggcc
     1921 gcggtgggaa agaacagcat gttctcgcgc ttcggcgtgc gcgcggggca aatcggacgg
     1981 cagtagacgc ctgtggtctt cacgccggaa aacagccggc catcgaagcg cggatcgcga
     2041 gtgcaaaaag cccggtagca cgcgtcgatg tcgaggtcca cgttcaatcg tcctcttctc
     2101 ccgccgaccg tgtctggcgg ttttcggacg tgtacagcac ggttcattgt ccgcgtttcc
     2161 gcgcctccgc ggttcgtaca tgaaaacggc cggtccaaaa ggaccggccg ttgccggtcc
     2221 ggctgccgcc ttcgcgccag gcgcttcggc gggccaagga agccagacgc gtacaagaag
     2281 agaggcacga agactctgtg tctccgtgcc tctagcttgc cccgccgtag ccttggcgaa
     2341 ggcgggtggt tcgtgaccgc tactcgacgt cgccggccgg cggacggccc agcatcggct
     2401 cgtcggtgtc gaacgcgtac tcgatctcgc gatccagatc gagctcgtcc tcggtcggct
     2461 gcggcggcac cggcggcggc ggcggctcgt ccgccggaat ccgcacgtgc cgataccact
     2521 cgaagcccgt gcccgcgggg atgagccgcc ccatcgtcac gttctccttc agcccgcgca
     2581 ggtggtcgat cttcccggag atcgacgcct cggtcagcac cctcgtcgtc tcctggaacg
     2641 aggccgccga gatgaacgag tcggtcgaga ggctcgcctt cgtgatcccc agcagcagcg
     2701 gctgcgccgt cgccggccgc cccccggcct gaatgcagcg ctcgttctcc tcgaggaacc
     2761 ggaacttgtc cgtctgctcg tcgatcagga actcggtgtc gcccacgtcc tcgatccgca
     2821 cccaccgcat catctgccgc gagatgacct cgatgtgctt gtcgttgatg ttgacgccct
     2881 gcag
//
LOCUS       DBNGSH010000203         1561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2223460, whole genome shotgun sequence.
ACCESSION   DBNGSH010000203 DBNGSH010000000
VERSION     DBNGSH010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2223460"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="AC16CY_04570"
     CDS             complement(<1..556)
                     /locus_tag="AC16CY_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353085.1"
                     /translation="MKCPKCSYLGFETGDRCRNCGYDFSLLAAPEPDHGELSLKPQTG
                     DLPVGELWLESPSKAVTVEAATSRPPSPLETPIPLFARPAVDHDEPLITLPAEPRAPL
                     SVRRAPETQPRSRPAVRPPRFSDDVGLVLEFAEDAPRRAESESRPPVAPEITRPAGLG
                     RRVSAALTDHAILLGVDAIVVYSTL"
     gene            complement(553..1281)
                     /locus_tag="AC16CY_04575"
     CDS             complement(553..1281)
                     /locus_tag="AC16CY_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353086.1"
                     /translation="MLSGLDFYFGGNYEQAINIWTRVAFLERGHGRARAYIERARGAL
                     AERQRESEELLHNGVAAYQAGNLQSARELLTRAIESGGTNETALLFLQRLGRLDPSTR
                     PSGQLSPSPRFGSSRDAADRSAAVPTRWFATVVASGVVAAVILLGALGAASWLRQWPV
                     AAPAADPAPVEPLPIERGSDARIARARQLLDAGRPAEALRELDAVHVDGAVGGEADQL
                     RSVIQRVLLSAVRPAAAEAGEGVR"
BASE COUNT          253 a          538 c          539 g          231 t
ORIGIN      
        1 ggagcgtcga gtagaccacg atggcgtcca cgccgagcag gatcgcgtga tcggtgagcg
       61 ccgcgctgac ccgacgtccg aggcccgcgg gtctcgtgat ctcgggcgca accggcggcc
      121 gcgactctga ctcggcgcgg cggggcgcat cctcggcaaa ctcgagcacc agtcccacat
      181 cgtccgagaa gcgcggaggc ctgactgccg gacgtgatcg cggctgtgtc tcgggcgcgc
      241 ggcgcacgga cagcggcgcc ctgggctcgg cgggcagcgt gatcaacggt tcatcgtgat
      301 ccacggcagg ccgcgcaaac agtggaatcg gcgtctccag gggagacggt gggcggctgg
      361 tggccgcctc caccgtcacg gccttactcg ggctctccag ccacagttca ccgacgggca
      421 ggtcccccgt ctgcggcttg agcgagagct ccccatggtc aggctccggc gccgccagaa
      481 gagagaagtc gtagccgcag ttgcggcagc gatcgcccgt ctcgaaacca aggtagctgc
      541 atttcgggca cttcatcgca cgccctcccc ggcctcggcg gccgccggcc tcacggccga
      601 gagcaacacc cgctgaatca cggagcgcaa ctggtccgcc tcgcctccca ccgcaccgtc
      661 gacgtgcacc gcgtcgagct cccgcagcgc ctcggccggg cgtccggcgt cgagcagctg
      721 gcgcgcacgg gcgattcgcg cgtccgatcc ccgctcgatc ggcagcggct ccactggagc
      781 cgggtctgcg gcgggcgcgg cgacgggcca ctgtcgaagc cacgacgcgg cccccaacgc
      841 gccgagcaga atcacagccg cgaccactcc gctggcgacg acggtcgcga accatctcgt
      901 gggaacggcc gcagatcgat ccgccgcgtc acgcgacgat ccgaatcgag gggagggcga
      961 cagctggccg gatggcctgg tggatgggtc gagtcggccg aggcgctgca ggaacagcag
     1021 ggccgtctcg ttggtgcctc cgctctcgat cgctcgtgtc agcagctcgc gcgccgactg
     1081 caggttgccg gcctggtagg cagccacgcc gttgtgcagc aattcctccg attcgcgctg
     1141 gcgttcggcg agggcgccgc gtgcacgctc gatgtaggcc cgtgcccgtc cgtgtccgcg
     1201 ttcgaggaac gcaacgcgcg tccagatgtt gatcgcttgt tcgtaattgc cgccgaagta
     1261 aaagtcgagg ccggagagca acagctcctc aatgcgctgg gcgcgatcgg actcgctcag
     1321 cgggccaggg tcggcaggtt cagtgcgctg cggctctgac atcccggaag gacgcgacgc
     1381 ccgatgcgtc gcgcacagcg attatacacg gggtcgggag tcatttcttg aggacactca
     1441 tggggacgtg atggcgagag tcgtccaaga aatgactccc gaccccatta acattaggac
     1501 gcatgctcat cgagtgcgtc cccaacatca gcgagggccg ccggcgtgac gtcgtcgccg
     1561 c
//
LOCUS       DBNGSH010000204         5615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_185759, whole genome shotgun sequence.
ACCESSION   DBNGSH010000204 DBNGSH010000000
VERSION     DBNGSH010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_185759"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1943
                     /locus_tag="AC16CY_04580"
     CDS             <1..1943
                     /locus_tag="AC16CY_04580"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3353087.1"
                     /translation="PTMDLAGRMLGLSPYFEFADSFAETNDPVRGAVDPTYVPVGHLG
                     GTYAAYYVVAHKDVAGWVASNALVDVSGGIEIHPVKAGCINGTDVIIWNPPLALGNYD
                     VVVDFGMMPAASAATYMTDAQYNDTLDFLDGADQIGFIVAKDPYELGAFAVGEDSYTQ
                     DDFFATLGSRANVDLRAVVRYPATMAGAGTPVAPGQHPLFVIEHGNHSSCNTGGFDFN
                     PAYDALHTGCPDREQNHMGYMGLLQRLASHGIIAVSIDAYDLTGNVPQLIAERSDLIL
                     KHIELWSHMNNTATFTGYPDFFAGRFAGHVDMTTISVSGHSRGGEASVGAYVRNAMSA
                     SPFAIGSVSSIAPVDGLSFVLPDVPYFVILPAADGDVCPLSGQRIYDRAGSSLAPVDS
                     TTKSGIYVYGANHNFFNTVWAGDGDDGAAGRDDFIPAADQQRLGEAFLSAFTRVHVKN
                     ESVYEDLLRGKLKFPSFAGRKAFAIRHEKTNRKLENGSGAGASAAAPATATSVASPSV
                     HETQALRVGWSGGSGTVTYTLSGAQRDASTFEVLSFRVAQTNAMTNPAGDQDFMVELT
                     GGGNTKATFSSQFGRVPRPYNHVAWCTPPLQNVMTTIRIPLHSFIMNKAGVTLDNLDA
                     VRIRFSNPGQGEMYVDDLEFSR"
     gene            1921..2421
                     /locus_tag="AC16CY_04585"
     CDS             1921..2421
                     /locus_tag="AC16CY_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353088.1"
                     /translation="MISSSLGSRAGAGLVVAIVAAGGAVCRAQAPDSPRPAQTQAAAS
                     KSEDDSAYRGRIELRAVRVRRLDATLEKMAGPEFLKRAKEPLAVEAQTARPLPTTARG
                     TSAILILNGERVADTWTILPDILVAFVDRSTLRQANTAAAAWSGAEQTSRSEKPITFQ
                     AEPIAK"
     gene            complement(2438..2785)
                     /locus_tag="AC16CY_04590"
     CDS             complement(2438..2785)
                     /locus_tag="AC16CY_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353089.1"
                     /translation="MPTSEELLALAAACADRLEERGQLRHVPSFSDVEEQCEEEYEAL
                     LQAIHEDEVFAPVDIQRQEQLVEQIASALPEDTRMLIVELADNHARHVWLQQEGAYHL
                     GLAVGRKLRKLAT"
     gene            complement(2824..3678)
                     /locus_tag="AC16CY_04595"
     CDS             complement(2824..3678)
                     /locus_tag="AC16CY_04595"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3353090.1"
                     /translation="MSSAPSFENLRVLILESRRAREMASIVASYGGTAISAPSMREVP
                     LESNTEALAFADALERGEYDLVILLTGVGTRALVAVVEGVRGSRKEFVAALRRTRVVA
                     RGPKPVAVLRELDVPVWLTAPEPNTWREVLASLDGRQSELPLRGVRVAVQEYGASNPD
                     LLSGLEARGATITRVPVYQWALPEDLGPLESACRALAGGGVDVVLFTTATQVVHLLKV
                     AQSMGLDEGVRAGLRTSLVASIGPTTTEELRQQGIAPDLESSHPKMGFLVREAAGRAA
                     DLLRTKRS"
     gene            complement(3829..5070)
                     /locus_tag="AC16CY_04600"
     CDS             complement(3829..5070)
                     /locus_tag="AC16CY_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3353091.1"
                     /translation="MNQADTAWMLIATALVLLMTPALAFFYGGLVRSKNALNTMMMSF
                     ISLGFVGVLWALIGYSFALSPGAGEGGNNWLGDMRFAFLNGVGLEAKDAVVPITIPHA
                     LYMAFQATFCIITAALISGAIVERMRFSAYLIFISLWSVVVYAPMAHWVWGGGWLQDM
                     GAWDFAGGTVVHVNAGAAALVAAMVVGKRTDYGHASILPHNIPTVLLGAGLLWFGWFG
                     FNAGSALVAGPIAGLAFVTTMLAPAATLVVWTFLDMMRSGKPTAVGCATAIVVGLVAI
                     TPAAGFISPMNAIILGAIAAVPSYLALIIRVKTGLDDSLDVVAAHGVGGTVGALLTGV
                     FAEKSLNGLFDGLIAGNPGQVGIQAAAVATAIVFSGGVSFILLKIIGLFIPLRATQSD
                     EATGLDVTMHGEEAYLHGGGE"
     gene            complement(5395..>5615)
                     /locus_tag="AC16CY_04605"
     CDS             complement(5395..>5615)
                     /locus_tag="AC16CY_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353092.1"
                     /translation="NQVDLRVAKVLRFSSNRLNVGFDLYNLFNSNPGLTFNQAYSETG
                     ATTGSGAWLAPQTILNARFIRFNATFDF"
BASE COUNT         1013 a         1863 c         1831 g          908 t
ORIGIN      
        1 gcccgacgat ggatctcgcg ggaaggatgc tcggcctatc cccgtacttc gagttcgcgg
       61 attcgttcgc ggaaaccaac gacccggtgc ggggcgccgt ggatccgacc tacgttccgg
      121 tcggccatct gggcggcacc tacgcggcct actacgtggt cgcccacaag gacgtcgcgg
      181 gctgggtggc ctcgaatgcg ctcgtggacg tgtcaggcgg gatcgagatc cacccggtca
      241 aggcgggatg catcaacggg acggatgtca tcatctggaa tcctccgctg gcgctcggca
      301 actacgacgt cgtggtggac ttcggcatga tgccggcggc atcggcggcc acctacatga
      361 ccgatgcgca gtacaacgac acgctcgatt ttctcgacgg cgccgaccag atcgggttca
      421 tcgtcgccaa ggatccgtat gagctcggcg cgttcgccgt aggtgaggac tcctacaccc
      481 aggacgactt cttcgcgacg ctcggcagcc gtgcgaacgt ggatctcagg gccgtggtgc
      541 gctatcccgc cacgatggcg ggcgccggga cgccggtcgc ccccggccag catccgctgt
      601 tcgtcatcga gcacggcaac cattcgtcgt gcaatacggg tggattcgac ttcaatcccg
      661 cgtacgacgc ccttcacacc gggtgtccgg accgtgagca gaaccacatg ggctacatgg
      721 gactgctcca gcgcctggcg agtcatggca tcatcgcggt gtccatcgac gcgtacgatc
      781 tcaccggcaa cgtaccgcag ctcatcgcgg agcgcagcga tctcatcctc aagcacatcg
      841 agctctggtc tcacatgaac aacacggcca ccttcaccgg ctatcccgac ttcttcgccg
      901 gccggttcgc gggccacgtg gacatgacca caatctcggt gtcgggtcac tcccgcggcg
      961 gcgaggcctc ggtgggcgcc tacgtcagga acgcgatgag cgcttcgccg ttcgccatcg
     1021 gctccgtgtc ctcgatcgcc ccggttgacg gcctgtcgtt cgtgctgccc gacgtgccgt
     1081 acttcgtcat cctgccggcg gccgacggcg acgtgtgccc gctcagcggc cagcggatct
     1141 acgaccgcgc gggcagctcg ctcgcgccgg tcgattcgac caccaagagc gggatctacg
     1201 tgtacggcgc gaaccacaat ttcttcaaca cggtgtgggc cggcgacggc gacgacggcg
     1261 cggcaggacg ggacgatttc attccggcgg ccgatcagca gcggctcggc gaggcattcc
     1321 tgtcggcctt cacgcgcgtg cacgtgaaga acgagagcgt ctacgaggac ctgctgcgtg
     1381 gcaagctcaa gtttccgtcg ttcgccggcc ggaaggcgtt tgcgatccgc cacgagaaga
     1441 cgaaccgcaa gctcgaaaac gggagcggag ccggcgcgtc cgccgcggct ccagcgaccg
     1501 cgacgagcgt ggcatccccg tccgtgcacg agacgcaggc gcttcgagtg ggctggagcg
     1561 gcggcagcgg cacggtcacc tacacgctgt ccggcgccca gcgcgacgca tccacgttcg
     1621 aagtgctgtc gttccgcgtg gcgcagacca atgccatgac caatcccgcg ggcgaccagg
     1681 atttcatggt cgagctcacc ggcggcggga acacgaaggc cacgttctcc agccagttcg
     1741 gccgcgtacc cagaccctat aatcatgttg cgtggtgcac gccgccgctc cagaacgtga
     1801 tgacgaccat ccggatcccg ctgcattcct tcatcatgaa caaagccggc gtgacgctgg
     1861 acaatctcga cgccgtgcgc atccggtttt ccaaccccgg ccagggagag atgtatgtcg
     1921 atgatctcga gttctctcgg tagccgcgcc ggggcggggc tggtcgtggc catcgttgcg
     1981 gccggcggcg cggtctgccg cgcgcaggcg cccgattcgc cgcgtccggc acagacgcag
     2041 gcggctgcgt cgaagagcga ggacgactcc gcctatcgag gacggatcga gctgagggcg
     2101 gtcagagtcc ggcggctgga cgcgacgctc gagaaaatgg cggggcccga gttcctgaag
     2161 cgcgccaagg agccgctggc cgtcgaggca cagacggcga gaccgctgcc gacgacggcg
     2221 cgaggcacgt ccgcgatcct gattctcaac ggcgagcgcg tcgccgacac gtggacgatc
     2281 ctgcccgaca tactcgtggc gttcgtcgat cggagcacgc tccggcaagc caatacggcc
     2341 gcggcggcct ggtccggagc ggaacaaacc agccgcagcg agaagccgat cacgtttcag
     2401 gcggagccga tcgccaaata gcgtcgcgcg gcgaggatca cgtcgcgagc ttgcgcagct
     2461 tgcgccccac cgccaggccc aggtggtacg cgccctcctg ctgcagccac acgtgccgcg
     2521 cgtggttgtc ggccagctcc acgatcagca tgcgcgtgtc ttccggaagc gcggacgcaa
     2581 tctgctcgac gagctgctcc tgacgctgga tgtccacggg cgcgaacacc tcgtcctcgt
     2641 gaatggcctg cagcagcgcc tcgtactcct cctcgcactg ctcctcgacg tccgagaacg
     2701 acggcacgtg gcgaagctgt cctcgctctt ccagcctgtc ggcgcacgcc gccgccagcg
     2761 cgagcagttc ttctgatgta ggcatggggg gatcttaaac ggtgcgaagg tgcgagggtg
     2821 cgattacgag cgcttggttc tgagcaggtc agccgcgcgc ccggcggcct cgcgcaccag
     2881 gaagcccatc ttgggatgcg atgactcgag gtccggcgcg atgccctgct gccgcagctc
     2941 ctccgtcgtc gtcggcccga tcgacgccac gagggatgtc cgaagcccgg cccgcacacc
     3001 ttcgtcgagg cccatcgact gagccacttt caagaggtgc accacctgcg ttgccgtcgt
     3061 gaagagcacg acgtccaccc ctccgcccgc gagggcgcgg cacgcggact cgagcggccc
     3121 gaggtcctcg gggagcgccc attggtagac ggggaccctg gtgatcgtcg cgccccgggc
     3181 ctcgaggccg gacagcagat cggggttcga cgcgccgtac tcctgcacgg caactctcac
     3241 gccgcgcagc ggcagctcgc tctgccgccc gtcgagcgac gcgagtactt ctcgccaggt
     3301 gtttggttcg ggagcggtca gccagacggg gacgtcgagc tcgcgaagaa cggctaccgg
     3361 cttcggtccg cgcgcgacga ctctggtccg gcgaagcgcc gcgacgaact ccttgcgcga
     3421 tccccggacg ccctcgacca cggcgacaag cgcgcgcgtg ccgacgcccg tgagcaggat
     3481 caccaggtcg tactcgccgc gctcgagggc atcggcaaac gcgagcgcct cggtgttgga
     3541 ctcgagcggc acctcgcgca tcgacggcgc ggagatcgcc gtccctccgt acgacgcgac
     3601 gatcgacgcc atctcccggg cgcgtcggct ctcgaggatc aggacgcgta ggttctcgaa
     3661 actgggcgct gaggacattg ttgattcgtg atctgtgatc gtcgattcgt tggcggactt
     3721 ctgattatcg atcgtcgatt tattgctgat tcacacaata aatctcaaat caccaatcaa
     3781 caatctccaa cgaatcaacg atcaccaatc aacaaatcac caatgttctt actcgccgcc
     3841 gccgtgcagg tatgcctcct cgccgtgcat cgtcacgtcg agaccagtcg cctcatcgga
     3901 ctgcgtggcg cgcagcggga tgaacagacc gatgatcttg agcaggatga agctgacgcc
     3961 gccgctgaac acgattgccg tcgcgactgc cgcggcctgg atgccgacct ggccgggatt
     4021 gccggcgatc aggccgtcga acaacccgtt caagctcttc tctgcgaaca cgccggtgag
     4081 gagcgcaccc acggttccgc cgacgccgtg cgcggcgacc acgtcaagcg agtcgtcgag
     4141 ccccgttttc acgcggatga tcagcgcgag gtagctcggc accgcggcga tggcgcccag
     4201 gatgatggcg ttcatcgggc tgatgaaacc ggcggccggt gtgatggcca ccagaccgac
     4261 gacgatggcc gtggcgcaac cgacggcggt tggctttccc gagcgcatca tgtccaggaa
     4321 cgtccagacg accagcgtgg cggcaggggc gagcatcgtc gtcacgaagg ccaggccggc
     4381 gatggggcct gccaccaacg cgctgccggc gttgaaaccg aaccagccga accagagcag
     4441 gccggcccca aggagcactg tgggtatgtt gtgcggaagg atcgacgcgt ggccgtagtc
     4501 tgtacgcttg cccacgacca tggccgccac cagcgccgcg gcgccggcgt tgacgtggac
     4561 gaccgttccg ccggcaaagt cccatgcgcc catgtcctgc agccagccgc cgccccacac
     4621 ccagtgcgcc atcggcgcgt agacgacgac cgaccagagc gagatgaaga tcagataggc
     4681 cgagaagcgc atgcgctcga cgatggctcc ggagatcagc gccgccgtga tgatgcagaa
     4741 cgtggcctgg aaggccatgt agagcgcgtg aggaatagtg attggcacga cggcgtcctt
     4801 ggcctcgagc ccgacgccgt tgagaaacgc gaagcgcatg tcgccgagcc agttgttgcc
     4861 gccttcaccg gcaccggggc tgagcgcgaa ggagtaaccg atcaacgccc acagcacacc
     4921 gacgaacccc agggagatga aactcatcat catcgtgttc agcgcgttct tgctccgcac
     4981 caggccgccg tagaagaacg ccaacgccgg cgtcatcagg agcaccaggg ccgttgcgat
     5041 caacatccac gcggtatctg cttgattcat gcactcacct cactgacggc gcggcgtgac
     5101 gatcacgcgc ccgagcaagg ccaattctag atgacggtca acgtcaaaag atcagcgatc
     5161 ggaggcacaa aaatgtccga ttggcattct gcgccgccat aagtgtctga actggacgga
     5221 tcgtgagggg gcagggggca ggggcgaggg ggcacggagt gcaggggggc acgggttgca
     5281 gggtgcacgg gtgcttgaaa aaaagaacgg gcgggacctc ccatctggga agccccgccc
     5341 tgtcggtctt cttcggcctg agccctgagg cctgaggcct gggcccttcc gctatcagaa
     5401 gtcgaacgtc gcgttgaacc tgatgaagcg cgcgttgaga atcgtctgtg gcgccagcca
     5461 ggccccggaa ccggtcgtcg ctccggtctc cgaatacgcc tggttgaacg tcaggcccgg
     5521 gttgctgttg aacaggttgt agaggtcgaa cccgacgttg agccggttgc tcgagaagcg
     5581 cagcaccttg gccacacgca gatcgacctg gttga
//
LOCUS       DBNGSH010000205         2208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1883986, whole genome shotgun sequence.
ACCESSION   DBNGSH010000205 DBNGSH010000000
VERSION     DBNGSH010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1883986"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC16CY_04610"
     CDS             complement(<1..226)
                     /locus_tag="AC16CY_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353093.1"
                     /translation="MTRAACVCATALLMYALTLTGAAQSVYPTGTTIYDPGSAWNGYT
                     VLSPLGTQAVLVIDMNGNVVKRWDNFNNSAG"
     gene            281..1789
                     /locus_tag="AC16CY_04615"
     CDS             281..1789
                     /locus_tag="AC16CY_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR3353094.1"
                     /translation="MNADSVRWGTATARWVLLATVLGSGIAFLDATVVNVALPAIGND
                     LGASVAGLQWIVNGYALTLSSLILIGGSLGDRFGRRRVFLIGVVWFAVASLLCGLAPT
                     SEALVAARALQGVGGALLTPGSLAILQSSFAPSDRGRAVGAWSGLSGIAAAIGPFVGG
                     WLIGTGSWRLIFLINLPLAAAVVAVTLRHVPESRDPAAGRAIDVAGAVLTAIGLAGVT
                     WALIEGGERGANAVTLSAGLAGLAALVGFVALERRVRDPMLPLDIFDSRQFTAANLVT
                     FVVYASLGVMFFLLVVHLQQVLGYSPLQAGVATLPVTALMLILSARAGSLADRIGPRL
                     PMTLGPLIIAVGLALLSRANAGSTYAGSVLPGILVFGLGLSLTVAPLTAAVLAAAPAR
                     HAGVASGVNNAIARGAGLLAVAAIPGLTSLTGNAYRDPAVFASGFRSAMLLSAALAAA
                     GGVIAWSLIRNEVAGRADACPAARLDRRHYCAVDGPPLATDRDAAEATRPAA"
     gene            1895..>2208
                     /locus_tag="AC16CY_04620"
     CDS             1895..>2208
                     /locus_tag="AC16CY_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353095.1"
                     /translation="MTSRSARWLSIGCALGLWAVGSAGPLSGQSASPRQTASSQSAAG
                     ATVRLKPDTTSATRQKPDTTSARALLDKYCVTCHSDRVKTANLSLQGLDLASVADHAE
                     VW"
BASE COUNT          281 a          734 c          761 g          432 t
ORIGIN      
        1 cgcccgcgga attgttgaaa ttgtcccagc gcttgacgac gttgccgttc atgtcgatca
       61 cgagcaccgc ctgcgtgccg agcggcgaca gcaccgtgta gccgttccac gcgctgcccg
      121 ggtcgtatat ggtcgtcccg gttgggtaga cggactgggc ggccccggtc agcgtcaacg
      181 cgtacatcag gagggcagtg gcacatacgc aggcggctcg tgtcatggcc aagaggatag
      241 gacggatcag cccggtctgt gcaagactcc ttgaggcgtc atgaatgcgg actccgtgag
      301 gtggggaaca gccactgccc gctgggttct gctggccaca gtactcgggt cgggcattgc
      361 cttcctcgat gctacggtcg tgaatgtcgc cctgccggcg atcggcaacg acctgggcgc
      421 gagcgttgcc ggcttgcaat ggatcgtcaa cggctacgcg ctcacgctgt cgtccctgat
      481 tctcatcggt gggtccctgg gtgaccgttt cgggcgccgg cgggtcttcc tcatcggagt
      541 cgtgtggttc gctgtcgcgt cgctcctctg cggcctggcg cccacgtctg aggcgctcgt
      601 tgcagcccgt gcgctccagg gtgtcggcgg ggcgctgctg acgccaggca gcctcgcgat
      661 ccttcaatcg tcattcgccc ccagcgaccg tgggcgcgcg gttggcgcat ggtcggggct
      721 ctccgggatc gccgccgcca tcgggccatt cgtcggcggt tggctcatcg gcacgggatc
      781 gtggcgtctg atcttcttga tcaacctgcc gctcgctgcc gctgtcgtcg ccgtgacact
      841 ccgccacgtt ccggagtcgc gcgaccccgc ggccggccgt gccatcgacg tggccggcgc
      901 cgtgctgact gcgatcgggc tggccggcgt gacgtgggcg ctcatcgagg ggggcgagcg
      961 cggtgcgaat gcagtgactc tttcggctgg actggccgga ctggcggcgc tggtcggctt
     1021 cgttgcgctg gagcggcgtg ttcgtgaccc gatgctgccg ctcgacatct tcgattcgag
     1081 gcagttcacg gccgccaacc tcgtgacgtt cgtcgtctac gcgagccttg gcgtcatgtt
     1141 cttcctgttg gtcgtccatc ttcagcaagt gcttggctat tcgcccctgc aggcgggcgt
     1201 cgcgacgttg ccggtcacgg cactcatgct gatcttgtcc gcgcgggcgg gctcgctggc
     1261 cgatcggatc ggaccgcggc tgccgatgac gcttgggcct ctgatcatcg ccgtggggct
     1321 cgcgctgctg agccgtgcga acgcagggtc gacctacgca ggctccgttt tgccagggat
     1381 cctggtcttt ggccttgggc tgtcgttgac agtcgcgcct ctcaccgccg cggtgcttgc
     1441 tgccgctccg gccaggcatg cgggcgtcgc ttccggtgtc aacaacgcga tcgcgcgcgg
     1501 cgcagggctc cttgcggtcg cggcgattcc ggggctcacc agtctcaccg gcaatgcgta
     1561 tcgtgatccc gccgtcttcg cgagtggctt tcggtcggcc atgctgctca gcgcggcgtt
     1621 ggccgcggcg ggcggggtga ttgcgtggtc gctcatccgc aacgaggtcg ccggtcgggc
     1681 ggatgcatgc ccggccgcca ggctcgatcg ccgccactac tgtgcggtcg acggcccgcc
     1741 gctggccacg gaccgcgacg cggcggaagc gacacggcct gccgcctgac tctgacaggc
     1801 cctttgctcg tccgtcatga gcgttattcc ccccgggcat aagggatttc agcgcttgaa
     1861 ctgacgctcc ggccgtgcga ctctggcgca ggccatgacg tcccgatccg ctcgttggct
     1921 gtcgatcggc tgtgcactcg gcctgtgggc cgtcgggtcg gccggtccgt tgagcggaca
     1981 gtccgcaagt cccagacaaa ccgcgagttc acagtcggcg gctggcgcca cggtccggct
     2041 gaagccggac actacgtctg caacacgaca gaagccggac acgacgtcag cgcgcgcgct
     2101 tctggacaaa tactgcgtca cctgccacag cgaccgcgtg aagacggcga acctctcgct
     2161 gcaggggctc gacctggcct cggtcgccga tcacgccgaa gtgtggga
//
LOCUS       DBNGSH010000206         2365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2732889, whole genome shotgun sequence.
ACCESSION   DBNGSH010000206 DBNGSH010000000
VERSION     DBNGSH010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2732889"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="AC16CY_04625"
     CDS             complement(<1..1511)
                     /locus_tag="AC16CY_04625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3353096.1"
                     /translation="MQEVSLPDISSAAEPVQKHIRERYAALRAAVDRSGTPAEDLARA
                     YGDLGKLLVATEYFDVAEACLANARALAPGERRWPYFLAHLRRLRTDPAGAAGFFEQS
                     LARAPSDVPSLVWLAEMYIALNRPGDAEPLLAKAESLDSKSGAVLFGLGRVALARRDY
                     PRAVEYLERARAAGPQATLIHYPLALAYRGAGNRSKAEEYLRLRGEVGFPPVDPLLAE
                     LAGQLQNASAYEVQASQAIDEKRWTDAVASLRKASELAPDNAFTRLNLGTSLYMTGDA
                     AGALAEFQAAVRLSPGLSKAHFGIGVLMSEARRDQEAIDAFSRAVQADAGNAEARLAL
                     GDALRRSGRVQESLPQYAGVIKIDPSSSQASFGYAMALVRLRRYAEARDRLSEGASTF
                     PDQPGFAHALARLLAASPDDRVRDGARATAIMNDLMKTQQPSLGTVETMAMTLAEIGR
                     FDEAVQWQRQAIAVAAQLKRPDVTARLSKNLRLYEMRQPCRVPWTDDDPVHRPG"
     gene            complement(1628..>2365)
                     /locus_tag="AC16CY_04630"
     CDS             complement(1628..>2365)
                     /locus_tag="AC16CY_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3353097.1"
                     /translation="GDADGDGDEDLFMTELSGEGSNLFVNDGSGSFEDRSTPSGVGPA
                     SIALTGFGTAWIDFDNDGRLDLLSVNGTVQIIESLRQLRDPFPLHQRKLLFRNVDGGR
                     FEDVSARAGAAFASSEVGRGAAFGDIDNDGDVDVLVGNNNGPARLLLNQLPGRHWIGL
                     RLAGPNGRDMLGARAEIARKGAPSLWRRARADGSYASANDPRIVAGLGDSTEPPDVRV
                     IWPDGRVETWTSVAIDRYTTLTQGSGK"
BASE COUNT          379 a          812 c          803 g          371 t
ORIGIN      
        1 cctggtcgat gaacaggatc gtcgtcggtc cacgggacgc ggcagggttg ccgcatttca
       61 tagagccgca gattcttcga gaggcgcgcc gtgacgtcgg ggcgtttcag ctgcgccgcc
      121 acggcgatgg cctgccgctg ccactggacg gcctcgtcga acctccctat ctccgccagc
      181 gtcatcgcca tcgtctcgac ggtgccgagc gacggctgct gcgtcttcat gaggtcgttc
      241 atgatcgccg tggcacgcgc accgtcgcgc acccggtcgt cgggagaggc ggcgagcagg
      301 cgcgccagtg cgtgcgcgaa tccaggctgg tccgggaacg tgctggcgcc ttcgctcaac
      361 cggtctcgcg cctccgcgta acggcgcagc cggacgaggg ccatggcgta gccaaagctg
      421 gcctgcgacg acgacggatc gatcttgatg acgccggcat actgcggcag cgactcctgg
      481 acgcgtccgc tccgacgaag cgcgtccccc agcgcgagcc tggcttcggc gttgcccgcg
      541 tccgcctgga cggcccgcga gaacgcgtcg atcgcctcct ggtcccggcg ggcttcgctc
      601 atgagcacgc cgattccgaa gtgcgccttg gacaaccccg gcgacaggcg gacggcggcc
      661 tgaaactctg ccagcgctcc ggcggcgtcg cctgtcatgt acagcgaggt gcccaggttg
      721 agcctcgtga acgcgttgtc aggagccagt tcgcttgcct tgcgcagact cgcgacggcg
      781 tccgtccatc tcttctcgtc gatcgcctgc gacgcctgga cctcgtaggc ggaggcgttc
      841 tgcagctggc cggcgagctc cgcgaggagc ggatcgaccg gcggaaaccc gacttcgccg
      901 cgcagtcgca ggtactcctc cgccttggag cggtttccgg caccacggta ggcgagcgcc
      961 agcggatagt gaatcagggt tgcctgaggt cccgcggccc ttgcccgttc cagatactcg
     1021 accgcgcgcg gatagtcgcg cctggcgagc gccacgcgtc ccaacccgaa cagcaccgct
     1081 ccgctcttcg agtcgagtga ttccgctttc gcaagaagag gctcggcgtc gcccggacgg
     1141 ttgagcgcaa tgtacatctc ggcgagccag acgaggctcg gcacgtccga cggcgcgcgc
     1201 gcgagcgact gctcgaagaa cccggcggcg ccggctggat cggtccgcag ccggcgcaaa
     1261 tgggcgagaa agtacggcca gcgccgttcg ccaggcgcga gtgcgcgggc gttggcaaga
     1321 caggcctcgg ccacatcgaa atactctgtc gccaccagca gcttgccaag gtcgccatac
     1381 gcacgtgcaa gatcctccgc cggagtgcct gaccgatcaa cggcggcgcg gagcgcggca
     1441 tagcgttcgc gaatgtgttt ctggaccggc tcggccgcgc tcgatatgtc gggaagcgat
     1501 acctcctgca acgctgggcg aactggcgct gccggctcgg cggccgggga gcaggctgtc
     1561 acacaaagga cgaggagcgg tgcgacggta cgaaggggga agggggccag tgggcagggg
     1621 gaagcgatca cttgcctgaa ccctgcgtca gagtcgtgta ccgatcgatc gccacgctgg
     1681 tccacgtctc aaccctgccg tccggccaaa tgacgcgaac gtccggcggc tccgtcgaat
     1741 cgcccagccc cgcgacgatg cgcgggtcgt tggcggaggc gtagctgccg tcggcgcgcg
     1801 cgcgacgcca gagggatgga gcgcccttgc gcgcgatctc ggctcgcgcg ccgagcatgt
     1861 cgcgtccatt cggacccgcc agcctcagcc cgatccagtg ccggcccgga agctgattga
     1921 gaagcaggcg cgcgggcccg ttgttgttgc cgacgagcac gtccacatct ccgtcgttgt
     1981 cgatatcgcc gaaagcggcg ccgcgaccaa cctccgacga ggcgaaggcc gcacccgccc
     2041 gggccgagac gtcctcgaag cgtcctccgt ccacgttccg gaacaacagc ttccgctgat
     2101 gcagcggaaa cggatcgcga agctggcgca ggctctcgat gatctgtaca gtcccgttca
     2161 ccgacagcag atcgagccgg ccgtcgttgt cgaagtcgat ccaggccgtg ccgaacccgg
     2221 tgagcgcgat cgaggcaggg ccgacgccgg agggagtgct gcggtcctcg aaggatccgg
     2281 atccgtcgtt gacgaacagg ttgctccctt cgccgctcag ctccgtcatg aacagatctt
     2341 cgtccccgtc gccgtccgcg tcgcc
//
LOCUS       DBNGSH010000207         2653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4261148, whole genome shotgun sequence.
ACCESSION   DBNGSH010000207 DBNGSH010000000
VERSION     DBNGSH010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4261148"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="AC16CY_04635"
     CDS             <1..1096
                     /locus_tag="AC16CY_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3353098.1"
                     /translation="VVVVAMLSSTIVPSSEGGQRQRGGDRGQGGQGRSEQRRAEPRDR
                     DGGRRASGEGPSPMARPVQEGRRAVERQQQPQAARNNNSRQMAETRQVREPRQVAAAR
                     QPEAIQRQDRRPSNRERSAQAAGVRDPGVVVAQRRPQERANATQGHFIDLDRGAPNRG
                     SASPTTRSTAPRSVPGVAPTRSYVDRNGYRSGSGSRDGNGYRDGNAYRGGNYRDYNGH
                     RGYKTYNYRGRTYNYYTIYPPPYYYYPYSYGYGPGGRGYFYFDLHYNSHVWYPHTTVR
                     YDSYGTYGYPIGELRLQVRPREAQVFVDGSYAGTVDEFDGTFQSLRLEGGEYQIEIVL
                     EGFEPLVFDVRITPGEKVTYRGDLVPEQPQ"
     gene            complement(1182..2552)
                     /locus_tag="AC16CY_04640"
     CDS             complement(1182..2552)
                     /locus_tag="AC16CY_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3353099.1"
                     /translation="MTTNPTQSLERRLGPLDAAAIIVSNVIGTGLLFAPSQVAAAVPS
                     ALPFLAAWLVGGVLAFAGAMAYAELAALRPRAGGEYVYLKEAFGPQAAFLTGWTSFVA
                     GFSGAIATSAMVLAFYLGRFVPAAGNSTPFFTLPIVPGTLELSLSPQTTVAIAAIVGM
                     SWIHLRGVGPGRLVMDVLATLKVGAFVLFIALGFSFGTGTAANLGQPGSATATGWLLA
                     LIPVMFAYSGWNAATYVAEEIRNPGRNVPRALALGTVAVVLVHVLMNALYLYVMPVGD
                     LGRVQGSVLDFVAEGLLGSGAGNIMGVVSIVSLLASISAMTFAGPRVYFAMARDGLFF
                     RRAADVHRGYKTPAAAIIAQALWSSLLVLSAKADALLTYTGFAIVLFSGVAVVSLFVL
                     RARQPQAERPFRAWGYPIAPGVFAVASAAILINGLVRDPGPTGAGALIILAGIPLYFF
                     FRKRGG"
BASE COUNT          523 a          870 c          888 g          372 t
ORIGIN      
        1 tgtggtcgtg gtcgcgatgc tgtcgtcaac catcgtccct tcgtcggaag gcggccagcg
       61 ccagcggggc ggcgaccgcg ggcaaggcgg gcaaggtcga tccgagcagc gtcgggccga
      121 gccgcgcgac cgcgacggcg ggcgccgcgc ctctggcgaa ggaccgtcac ccatggcgcg
      181 acccgtccag gaaggacgac gcgccgtcga acggcagcag cagccgcaag ccgcccggaa
      241 caacaactcg aggcagatgg ccgagacgag gcaggtgagg gagccgcgac aggtggcggc
      301 ggcccgtcag cccgaggcga tccagcgcca ggaccggcga ccgtcaaacc gggagcggtc
      361 ggctcaggcc gccggtgtcc gtgatcctgg tgtcgttgtc gcacagcgcc ggccccagga
      421 acgggccaac gcgactcagg gtcatttcat cgatctggat cgtggtgcgc caaaccgtgg
      481 ctcggcctcg ccaaccactc gctcgacagc cccgcgaagc gttccgggag tcgcgccgac
      541 gcgcagctac gtcgaccgta acgggtatcg cagcggcagc ggttctcgcg acgggaacgg
      601 ctatcgcgac gggaacgctt atcgaggcgg gaactatcgc gattacaacg gccatcgtgg
      661 ctacaagacc tacaactacc gcggccggac ctacaactac tacacgatct acccgccgcc
      721 ctattactac tatccgtatt cctacgggta cggcccgggt ggacgcgggt acttctactt
      781 cgacctgcac tacaactcgc atgtctggta tccgcacact accgtccggt acgattccta
      841 cggcacctac ggctacccga tcggcgaact gcggctgcaa gtgcgaccgc gcgaagcgca
      901 ggtgttcgtg gacggttcct acgcgggcac ggtggacgaa ttcgacggca cgttccagtc
      961 gctcaggctg gagggaggcg agtaccagat cgagatcgtc ctcgagggct tcgagccgct
     1021 cgtcttcgac gtgcgcatca cgccgggcga gaaggtgacg tatcgcggcg atctggtgcc
     1081 ggaacaaccg caataagcgc gggctgaagc ccgcgctcta caaacgaccg gtacgtcgat
     1141 gtacgaacga ccggtaccgt cgatgccgtg gcgcgggggc ttcagccccc gcgctttctg
     1201 aagaagaagt acagcgggat tcccgccagg atgatgagcg cgccggcgcc ggtcggccct
     1261 gggtcccgga cgagtccgtt gatgaggatg gcggcgctcg cgacggcaaa cacaccggga
     1321 gcgatcggat acccccaggc gcggaacggc cgctcggcct gaggctgccg ggcgcgcaac
     1381 acgaacagcg aaacgacggc gacccccgag aacagcacga tcgcaaatcc cgtgtaggtc
     1441 agcagcgcat cggctttggc cgacagcacc agcagggagc tccagagcgc ctgcgcgatg
     1501 attgcggcag ccggcgtctt gtacccgcgg tggacgtcgg cagcccgccg gaagaagagg
     1561 ccgtcgcgcg ccatcgcaaa gtagacgcgc ggaccggcaa aggtcatggc gctgatgctg
     1621 gccagcaggc tgacgatcga cacgaccccc atgatgttgc ccgccccgga accgagcagc
     1681 ccttccgcga cgaagtcgag gacggagcct tgaacccgcc cgagatcccc caccggcatg
     1741 acgtacagat agagggcgtt catcagaacg tggacgagta cgacggccac cgtgccgagt
     1801 gcgagtgcgc gagggacgtt gcgcccagga ttcctgattt cctcggcgac gtaggtggcg
     1861 gcattccatc ccgaatacgc gaacatcacc gggatgagcg cgagcagcca gccggtcgct
     1921 gtggccgatc cgggttggcc gagattggct gcggtccccg taccgaacga aaagccgagg
     1981 gcaatgaaca gaacgaatgc gcccactttc agcgtcgcca ggacgtccat caccagccgg
     2041 cccgggccaa cgccccggag gtgaatccac gacattccga cgatggcagc gatcgccacc
     2101 gtcgtctggg gcgacagact gagctcgagc gtcccgggaa caatcggcag cgtgaagaac
     2161 ggcgtgctgt ttccggccgc gggcacgaac cgtcccaggt agaaggcaag gaccatggca
     2221 ctggtcgcga tggcgccgga aaaacctgcg acaaacgagg tccagcccgt gagaaacgcc
     2281 gcctggggcc caaaggcctc cttgagatag acgtattcgc cgcccgcacg cggcctcagc
     2341 gcggccagtt ccgcgtaagc catcgcgccc gcaaacgcga gcacgccccc gaccagccat
     2401 gcggcgagga atggcaacgc gcttggaacg gcggccgcca cttgagacgg ggcaaacagc
     2461 aggcctgtgc caatgacgtt ggaaacgatg atcgccgccg catcgagggg accgagacgg
     2521 cgctcaaggg attgggtggg gttcgtagtc acccgcggat tatatgggcc ttggggcctt
     2581 cttactcagt gaggagctgc cggtcgatcg tgtagtcccc catcttcacc cagttgcggg
     2641 agccatgcct gcc
//
LOCUS       DBNGSH010000208         9547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_16542, whole genome shotgun sequence.
ACCESSION   DBNGSH010000208 DBNGSH010000000
VERSION     DBNGSH010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_16542"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC16CY_04645"
     CDS             <1..479
                     /locus_tag="AC16CY_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353100.1"
                     /translation="SRSVPGSMIPVMYGNNYQIVQSPGFVVVTYEIIHESRVIPLDGR
                     PHVGRAVRTHMGDPRGRWEGATLVVETTNFTDESAYRNSKGDTLRVTERFTRVAPGRI
                     EWTATMEDPMTWTRPWTIAMPLTADPHPILAFDCHEHNYGLRNILSAARAADKTEP"
     gene            610..1134
                     /locus_tag="AC16CY_04650"
     CDS             610..1134
                     /locus_tag="AC16CY_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353101.1"
                     /translation="MNGIDPAFLALFAAIGATIWFAAATRGRGSGALAAVGAVAVFGG
                     GLIASLGLAHTVAVIGRALRGPSFVYDFRLYSLLLLGACQIAGGLRCLSTSWRLARGD
                     ASAWRAALAATTLLLIVNVPLMPITGVAGITSLPSAVMLFTLIATGRRFVRRSDAETV
                     TIPGGIREHASSAT"
     gene            1193..1693
                     /locus_tag="AC16CY_04655"
     CDS             1193..1693
                     /locus_tag="AC16CY_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3353102.1"
                     /translation="MTWASADAAIAALFEEHARSLVRLARLFADDRNAAEDLVQEAFL
                     RLRRSFHRIDSPDRTAAYLRSIVLNLARDHNRRGLMSLRHRMPEEESRLSVEDRIVVR
                     EDQQQVIDALLALPRRQRDCVTLRYYLDLSIPDIAMTLGISVNSVKTHLQRGMAVLEV
                     RLEAAP"
     gene            1690..2328
                     /locus_tag="AC16CY_04660"
     CDS             1690..2328
                     /locus_tag="AC16CY_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353103.1"
                     /translation="MTRLEARLRDALHAGTLQVEESPDLFARVKGSIEDDARRRNWRR
                     RVAAITGTVLAAFAALLVAVTDFREGELFMDWRILELLTTTILVVLVIVLGPFIKRFG
                     KSYAAEVFRANPGTGKSFIVLMDFAYYLVFGAYVLFTPVLNPPATWSAYANAAQLKVE
                     MMKVAGMLLLMGVLHGVNLLLLPTIGRLLMLNKQLDAEMRDRERPKDHGGAA"
     gene            2438..2686
                     /locus_tag="AC16CY_04665"
     CDS             2438..2686
                     /locus_tag="AC16CY_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HIR3353104.1"
                     /translation="MLNRNSCALTPAQANLRGVAWVAGAFLICPCHLPLTLALATTAL
                     AGTTAGLVLGAHWLAAGVFITLAWLAATWHGIRLVRRR"
     gene            complement(2722..3336)
                     /locus_tag="AC16CY_04670"
     CDS             complement(2722..3336)
                     /locus_tag="AC16CY_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3353105.1"
                     /translation="MLRGIVLAAGDSVRMRAPKAVLPAADGRPFVARIAHTLREAGVA
                     DLAVVTGRDHEAVVAALRQAALPSLPIVVRNPDPSRGQLSSLWLGMDAVLDPGAEGLL
                     VTLVDVPMVAVSTVTAVIEAWRRTRAPIVRPAIGDRHGHPVIFDRALFDELRRAPLEA
                     GAKSVVRAHEPDILNVTVDDEGCLTDVDTPEDYRRIRGRALDGS"
     gene            3567..3773
                     /locus_tag="AC16CY_04675"
     CDS             3567..3773
                     /locus_tag="AC16CY_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3353106.1"
                     /translation="MPTGTIARLLIDKGFGFIRDEGGIEHFFHRSAVRQAVFELLREG
                     QRVEFTAEESTKGPRAGDVRLIEG"
     gene            complement(3844..4551)
                     /locus_tag="AC16CY_04680"
     CDS             complement(3844..4551)
                     /locus_tag="AC16CY_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3353107.1"
                     /translation="MPDEVLVQLRAVTKDYRGLRPLRIESLELRAGRSMALLGFDQAM
                     AEVLVDLITGAILPDSGDITAFGRPTTDIADPDSWLSTLDEFGLLTDRAVLVEHFTVA
                     QNLAVPLSLAVEDMPRQIRDRVGELASEVGLAGELDLQTGATSPSTRARIRLGRALAL
                     GPRVLLGEHPNATLSRDDAGAFAADLSRIVASRRLASLVLTADGDFARAVAEDVLVLK
                     PATGVLEPWSRWRRWFS"
     gene            complement(4541..5371)
                     /locus_tag="AC16CY_04685"
     CDS             complement(4541..5371)
                     /locus_tag="AC16CY_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3353108.1"
                     /translation="MIDLHLHTTASDGRCTPRELVDRVAAAGITVMAVTDHDTTAAFE
                     EVRACASERRIEAVSGIEITAVDDGRDVHVLGYFLDPSEPSLIQFLAAQRATRVARVH
                     TIAARLSELGVPIDLQALLDEAERNPGRSIGRPQVARALQQAGHVSGTQEAFDRFLAN
                     GGPAFVTRPGSSPEVVTGIIHRAGGLASLAHPGRTRIDARIPELAAAGLDALEVYHSD
                     HDEAAVERYRQLASGLGLMTTGGSDFHGDPARGLEPGASTVPMPEWQRFSSARRRHAR
                     "
     gene            complement(5413..6393)
                     /locus_tag="AC16CY_04690"
     CDS             complement(5413..6393)
                     /locus_tag="AC16CY_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HIR3353109.1"
                     /translation="MQAIPDLARLKPTGGKRSSKREQIVNVFLRQEGHLSADDLVDLI
                     RREDHRISRATVYRTLQWMVDAGIARKVDFGEGRFRFEHSYRQPRHFHMICKTCHHSF
                     EFLSSDIEASIEEVAAARSFSAAQSVVQIYGTCEECRTGRASTSGAEGHTDLVFARDA
                     LRIAIATERSGLDFYTRAARVTHDARGRKVFQDLALEEKEHLGKLEVRYSELLRRDPQ
                     LESRPTFLFFKGAANGLFAEGADRLLRGVNDQQALMIGIKCERGSHRFFKKYGERFED
                     SEGKQVFLEFADEERNHLDLLIREYRALVKRQGRQRRSARPASAARAGAR"
     gene            complement(6476..6838)
                     /gene="erpA"
                     /locus_tag="AC16CY_04695"
     CDS             complement(6476..6838)
                     /gene="erpA"
                     /locus_tag="AC16CY_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR3353110.1"
                     /translation="MQRPEFRIQEPDTMINVSPTAASKINELLAEEGKAGSGLRVFVQ
                     GGGCSGFQYGLMIEENGGDADQVYESNGVRLFVDPVSVSYLKGAEVDFVDTVTGGGFT
                     IKNPQATSTCGCGQSFSV"
     gene            complement(6966..8486)
                     /locus_tag="AC16CY_04700"
     CDS             complement(6966..8486)
                     /locus_tag="AC16CY_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HIR3353111.1"
                     /translation="MLPSFFDSVLSLVVRTSTDLPPDVRAAMKVAMAQEQAGTQSAQA
                     LAVIAQNIDLASDCEGAICQDTGMPTFEVKVPVGVNQIVLKEQIRRAVAEATRRGKLR
                     PNSVDSLTGENSGDNLGPGTPIVHFEQWENDDEIEVKLILKGGGCENMNAQYSLPAEL
                     TGLGRADRSLAGVRKCILHAVWQAQGKGCAPGAVGVCVGGDRTSGYVHAKEQLFRTLD
                     DVNPDPRLAELESSIMGEVNTLGIGTMGFGGRVSLIGCKIGALNRLPASFFVSVAYDC
                     WAYRRLGVILDSRSGAITRWLYRDASSPVIHMADQAGFPRTGREISLRTPLSEEQVRA
                     LKVGDVVLLSGKVYTGRDAVHHHLMKHDPPVDLRGGVIYHCGPVVAKEGDGWRVTAAG
                     PTTSIREEPYQADVIARNGVRAVIGKGGMGAKTLAALQQSGAVYLNAIGGAAQFYART
                     ITGVDGVSLIEFGTPEAMWHLSIENFPAIVTMDAHGNSLHRDVESASARQLAGIGG"
     gene            8572..>9547
                     /locus_tag="AC16CY_04705"
     CDS             8572..>9547
                     /locus_tag="AC16CY_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR3353112.1"
                     /translation="MTPDPISPTRVEHDSLGEYAVPSDAYYGIQTARAVENFPISGLR
                     APADLVTATVVIKGAAARANVELGRLARGVGDAIVKAADEIAGGALREQFVVDVYQAG
                     AGTSHHMNVNEVLANRAAELLGGSKGEYRLVHPNDHVNMGQSTNDVFPTATRLALLLG
                     HRPLVDAALALAASLHRKAMEFDGVLKVGRTHLQDAVPMTLGQEFGGYAACVQRGAED
                     VERAAEQLKELNLGATAVGTGLNAGDDFARRAVAHIAANVGMALAPAANRFRVTQSMG
                     DVLGYSSAVRRLAIEVSKVASDLRLLSMGPRAGLGEITLPAVQPGSSIM"
BASE COUNT         1564 a         3218 c         3142 g         1623 t
ORIGIN      
        1 tctcgcgaag cgtgccgggc tcgatgatcc cggtcatgta cggcaacaac taccagatcg
       61 tgcagtcgcc cgggttcgtc gtcgtgacct acgagatcat ccacgagagc cgcgtgatcc
      121 cgctcgatgg gcggccgcac gtggggcggg ccgttcgcac gcacatggga gacccgcgcg
      181 gacgctggga gggcgccacg ctcgttgtcg aaacgacgaa cttcacggac gagtccgcgt
      241 accggaattc caaaggagac acgcttcgcg ttaccgagcg cttcacccgc gtcgcacccg
      301 gccgcatcga gtggaccgcc accatggaag acccgatgac gtggacgagg ccgtggacca
      361 tcgcgatgcc gctgaccgcc gatccgcatc ccatccttgc gttcgattgt cacgagcaca
      421 actacgggct ccggaacatc ctgagcgcgg cgcgggccgc agacaaaaca gagccataac
      481 gtcacgacac accgaggctc cgaggcactg aggtcagaac gcaaagattt cctcggtgtc
      541 acccgtttcc cgcgcctctt cgtctgtatc aatagacaac caaggacggg acaaagcaca
      601 ggtggtctca tgaacggcat cgatccggcc tttcttgcgc tcttcgcggc gattggagcc
      661 acgatctggt tcgccgccgc cacgcgggga cggggctcag gcgccctcgc agccgtggga
      721 gccgtcgccg tgttcggtgg cggcctgatc gcgtcgcttg gtctggctca caccgttgcc
      781 gtaatcggtc gcgcgcttcg aggaccgtcg ttcgtgtacg actttcgtct gtactccctg
      841 cttctgttgg gcgcgtgtca gatcgcgggt ggcctgcgct gtctgagcac gtcgtggcgt
      901 ctggcccgcg gcgacgcgag cgcatggaga gcggctctgg ccgcgacgac gctgctcctg
      961 atcgtcaacg tgccgctcat gcccatcacg ggggtcgccg ggattacgtc tctgccctcg
     1021 gcagtgatgc tgttcaccct gattgcgacg ggtcgtcgct ttgttcggcg cagtgacgct
     1081 gaaaccgtca caattcccgg gggcatacgc gagcacgcgt cctcggcgac ttgaagaggc
     1141 aggcctatgc tccggatgtg cctcgacgac aagaactggc caaggagcgt tggtgacgtg
     1201 ggctagcgcg gacgcggcga tcgcggccct cttcgaagaa cacgcgcgtt cgctcgtgcg
     1261 gctggcgcgg ctcttcgccg acgaccgcaa cgccgcggag gacctggtcc aggaagcgtt
     1321 tctccgtctt cgacgctcgt ttcaccgtat cgacagcccc gaccggacag ccgcgtatct
     1381 ccgctcgatc gtgctgaacc tggcgcgcga tcacaaccgg cgcgggctga tgtcgctgcg
     1441 gcaccggatg ccagaggagg agagccggtt gtccgtggaa gaccggatcg tcgtgcgcga
     1501 ggaccagcag caggtgatcg acgcgctgct ggcgctcccg cggcgccagc gcgactgcgt
     1561 gacgctcagg tactacctgg acttgagcat cccggacatc gcgatgacct tgggcatctc
     1621 ggtgaactcg gtcaagacgc acctgcagcg cggcatggcg gtgctcgaag tgcggctgga
     1681 ggcggcgcca tgactcgact cgaggcccgc ctgcgcgacg cgctccacgc gggaacgctg
     1741 caggtcgaag agagccccga tctgttcgcg cgtgtcaaag gctccatcga agatgacgcg
     1801 cgccgtcgga actggcggcg gcgagtggcg gcgatcacgg ggacggtact tgcggcattc
     1861 gcggcgctgc tcgtggcggt cacggacttt cgcgaaggag aactgttcat ggactggcgg
     1921 attctcgagc ttctcaccac gaccatcctc gtggtcctcg tcatagtgct gggccctttc
     1981 atcaagaggt tcgggaagag ctatgccgcg gaagtcttca gggcgaaccc cggtactggc
     2041 aaaagcttca tcgtgctgat ggacttcgcc tactacctcg tcttcggtgc gtatgtgctg
     2101 ttcacaccgg tgttgaaccc gccggcgacc tggagtgcct acgcaaacgc tgcgcagttg
     2161 aaggtcgaga tgatgaaggt cgccggcatg ttgctcttga tgggcgtgct gcacggcgtc
     2221 aacctgctcc tgctgccgac catcgggcgt ctgctcatgt tgaacaagca gctcgacgcc
     2281 gaaatgcgcg atcgcgaacg gccgaaagac catggggggg cggcatgatt ctcgtggctg
     2341 tctgtgtcag tgtccttctg gcgatcatcg ccgccgtggc gttcctcgga ctgcggcctc
     2401 tcctcaagcg caacagacgg gctggggagt gacgccaatg ctgaacagaa actcctgtgc
     2461 gctcactccc gcgcaggcca acctcagggg tgtcgcgtgg gtggcgggag cgtttctgat
     2521 ttgcccctgt cacctgccgc tcacgctggc actggccacc accgcgctgg cgggcaccac
     2581 agctggcctc gtgttgggcg cccattggct tgctgctggc gtcttcatca cgctcgcctg
     2641 gctcgcggca acctggcacg gtattcggct cgtgcgacga cggtgacgac ctcgactttc
     2701 cgcgcgtgcg ctcataccac gctatgaacc gtcgagtgcc ctccccctga tcctccggta
     2761 gtcctctggc gtgtccacgt cggtcaggca tccctcgtcg tccacggtca cgttcaggat
     2821 gtccggctcg tgcgcgcgga caactgattt cgcgcccgcc tcgagagggg cacggcgcag
     2881 ctcgtcgaac agcgcgcggt cgaagatcac cggatgaccg tgtcgatcgc cgatcgcggg
     2941 ccggacgatc ggggcgcggg tgcgccgcca ggcctcgatg acggcggtga cggtcgatac
     3001 ggcgaccatc ggcacgtcca cgagagtcac gaggagacct tcggcccctg gatcgaggac
     3061 ggcgtccatc cccagccaga gcgacgagag ttggccccgg gaggggtcgg ggttacggac
     3121 gacgatcggc agcgacggaa gcgcggcctg cctgagcgcg gcgacgacgg cctcgtggtc
     3181 ccggccggtg acgaccgcca gatcggccac gccggcctct cgcagcgtgt gcgcgatgcg
     3241 cgcaacgaag ggccggccgt cggcggcggg caacaccgcc ttcggggcgc gcatccgcac
     3301 ggagtcgccg gccgcaagaa cgattccccg aagcacgact catcctaatt cttgtttaac
     3361 aacgcttaac aacatcgttc aaataacagt gcaacaacgg gttctggcac ccttgacttc
     3421 ccccggcgga cgcccctaca cttgcgccac ttccgggccc tgtgttcggc ggatcgcttg
     3481 cggtcccaag gcattggcgt cgttgttcac ggtcagtccg ggagcgcctg ggattgtccc
     3541 aggccacgta tcggaggaaa acggccatgc cgaccgggac aatcgcacgc ctgctcatcg
     3601 acaagggctt cggcttcatc cgcgatgaag gtggcatcga acacttcttt catcgtagtg
     3661 ccgtgcgaca ggcggtcttc gagttgctgc gagaggggca gcgggtcgag ttcacggcgg
     3721 aggaatccac gaaaggtccg cgcgccgggg acgtcagact gatcgaaggc tagaacacct
     3781 cagtcgccgg cccctctggg gccggcgtga tcgcgccgca ggctttcccc ccaccgattc
     3841 ccatcacgaa aaccagcgac gccaccgcga ccagggctcg agcacgccgg tagccggctt
     3901 gagcaccagg acgtcctctg caaccgcgcg agcgaagtcg ccatccgcgg tcagcacgag
     3961 actggcaagc cgccgcgagg cgacgatgcg cgagagatct gccgcgaacg cgcccgcatc
     4021 atcccgagag agcgtcgcgt tcgggtgctc gccgaggaga actcgcggcc cgagcgcgag
     4081 cgcgcggcca agacggatgc gcgcccgcgt ggagggcgac gttgcccccg tctgcagatc
     4141 cagttcacca gcgagcccga cttccgaggc cagctccccc acgcgatcgc ggatctgcct
     4201 gggcatgtcc tcgacggcga gtgacaacgg taccgccagg ttctgagcca ccgtgaagtg
     4261 ctcgacgagg acggctcgat ccgtcagcag gccgaactcg tcgagcgtgg agagccacga
     4321 atccgggtct gctatatcag tcgtgggccg gccgaatgcc gtgatgtcgc cgctgtccgg
     4381 gaggatcgcg ccggtgatca gatcgacgag cacctcggcc atcgcctgat cgaagccgag
     4441 gagcgccatc gagcgtcccg cccgcagctc gaggctctcg atccgcagag gcctgagccc
     4501 tcgatagtcc ttggtgacgg cgcggagttg gacgagaacc tcatcgggca tgtcggcggc
     4561 gtgcgctgct gaacctctgc cactccggca tgggcacggt cgacgctccg ggctcgaggc
     4621 cgcgcgccgg gtcgccatgg aagtcggagc cgcccgtcgt catcagcccg aggccggatg
     4681 ccagctgccg gtagcgctcg accgccgcct cgtcgtgatc cgaatggtag acctcgagcg
     4741 cgtcgaggcc ggcggcggcc agctcgggta tccgcgcgtc gatgcgcgtg cggccgggat
     4801 gcgcgagcga ggcgaggccg cccgcgcgat gaatgatgcc ggtgacgacc tcgggcgacg
     4861 aaccgggtct cgtcacgaag gcgggtccgc cgttggccag aaagcgatcg aacgcttcct
     4921 gcgtgccgga gacgtggccc gcctgctgga gcgcgcgcgc cacctgcgga cggccgatcg
     4981 agcgccccgg gttgcgctcc gcttcgtcga gcagcgcctg caggtcgatc ggcacgccca
     5041 gctcggacag gcgcgcggcg atcgtgtgca cgcgggcgac gcgcgtcgcc cgctgggccg
     5101 cgagaaactg gatcagcgac ggttcggagg gatcgaggaa atagcccagc acgtggacgt
     5161 cgcgcccgtc atcgaccgcc gtaatctcga tgccggaaac cgcttcgatc ctccgctcgc
     5221 tggcgcaggc ccgtacctcc tcgaacgccg cggtggtgtc gtggtccgtc acggccataa
     5281 ccgtgatgcc ggcagcggcg acgcggtcca ccagctcgcg cggagtgcag cggccgtcgg
     5341 aggccgtggt gtggaggtgg aggtcgatca cccctcgacg aggctcgggg tgaccctgag
     5401 cgtgtcgaag ggtcaccgag cgccggcgcg ggccgcggac gctggacgag ccgaacggcg
     5461 ttggcgtccc tggcgcttga cgagcgcccg gtactcgcga atgagcagat cgaggtggtt
     5521 gcgttcctcg tcggcgaatt cgagaaagac ctgcttccct tcggaatcct cgaaccgctc
     5581 gccgtacttc ttgaagaacc ggtgcgagcc ccgctcgcac ttgatgccga tcatgagcgc
     5641 ctgctgatcg ttgacgccgc gaagcagacg gtcggcgccc tcggcgaaca gtccgttggc
     5701 cgcgcccttg aagaacagga aggtggggcg cgactcgagc tgagggtcgc gcctcagaag
     5761 ctcgctgtag cgcacctcca gcttgccgag gtgctccttc tcttcgagcg ccagatcctg
     5821 gaacaccttg cggccgcgcg cgtcgtgcgt cacgcgggcc gcgcgggtat agaaatccag
     5881 gccgctccgc tcggtggcga tcgcgatgcg cagcgcgtca cgcgcgaaga cgaggtcggt
     5941 atgcccttcg gcgcccgacg tcgaggcgcg gcccgtacgg cactcctcgc acgtcccgta
     6001 gatctgcacg acgctctgcg ccgcgctgaa gctgcgcgcc gcggcgactt cctcgatgga
     6061 cgcctcgatg tcggagctga ggaactcgaa cgagtgatgg caggtcttgc agatcatgtg
     6121 gaagtggcgg ggctgccggt acgagtgctc gaaccggaac cggccttcgc cgaaatccac
     6181 cttgcgggca atccccgcgt ccaccatcca ctgcagcgtg cgatagaccg tggcgcggct
     6241 gatgcgatgg tcctcgcgcc tgatcagatc gaccagatcg tcggcgctca ggtggccttc
     6301 ctggcgcagg aacacgttga caatctgctc ccgcttgctg gagcgcttgc caccggtcgg
     6361 cttcagtctt gcgagatctg ggatggcctg cacggcgggc gacctgaccc ttcgacctaa
     6421 cccttttgga tcttaccttt gaccttggac ctttgacctt ggacctggct cgggctcaga
     6481 cgctgaaaga ctgcccgcag ccgcacgtcg aggtcgcttg gggatttttg atcgtgaagc
     6541 cgcctccggt cacggtgtcg acgaaatcga cctcggcgcc cttcaggtag ctcacgctca
     6601 ccgggtccac gaacaggcga acgccgttgg attcgtacac ctgatcagcg tcgcccccgt
     6661 tctcttcgat catcaacccg tactggaaac ctgagcagcc gcctccctgc acgaacacac
     6721 gaagcccgct gccggccttt ccctcttccg cgagcagctc gttgatcttc gaagccgccg
     6781 tcggggacac gttgatcatg gtatctggct cctgaatcct gaattccggc ctctgcatga
     6841 ggccacgccg gttcgtacgc tcccggtcca gttcaaatta tacgatccgc cacgagtccg
     6901 cggcaaccgc ttacacctct actggccgcc gtaaattgct cctgcaccgc cacttagcca
     6961 tggactcaac cgccgatgcc cgccagctgg cgggccgatg cgctttcgac gtcccgatga
     7021 aggctgttcc catgagcatc catcgtcacg atggcgggga aattctcgat gctcaggtgc
     7081 cacatcgcct cgggagtgcc gaactcgatc agtgagacgc catcgacgcc ggtaatggtg
     7141 cgcgcgtaga actgggccgc tcccccaatc gcgttcagat acaccgcgcc cgactgctgc
     7201 agcgccgcaa gcgtcttcgc gcccatgccg cccttcccga tcacggcgcg cacgccgttg
     7261 cgcgcgatca cgtcggcctg gtacggctcc tcgcggatgc tcgtcgtcgg cccggccgcg
     7321 gtgacccgcc agccgtcccc ctctttcgcc acgacgggac cgcagtgata gatgacgccg
     7381 ccgcgcagat ccaccggagg atcgtgcttc atgagatggt ggtggacggc atccctgccc
     7441 gtgtacacct tcccggagag caggacgacg tcgccgacct tgagcgcgcg cacctgctcc
     7501 tcgctgagcg gcgtgcggag cgagatctcg cggccggttc gcgggaagcc ggcctgatcc
     7561 gccatgtgga tgaccgggct cgacgcgtcc cggtacagcc agcgcgtgat ggcgcccgag
     7621 cggctgtcga ggatgacgcc cagccgccgg tacgcccagc aatcgtaggc gaccgacacg
     7681 aagaagctcg caggcagtct gttgagcgcg ccaatcttgc agccgatgag cgaaacgcgg
     7741 cccccgaagc ccatcgtgcc gatcccgagc gtgttgactt cacccatgat ggacgactcg
     7801 agctccgcca ggcgcgggtc cgggttcacg tcgtccagcg tgcggaacaa ctgctccttc
     7861 gcatgcacgt atcccgatgt acgatcgccg ccgacgcaga cacccacggc gcccggcgcg
     7921 catcccttgc cttgagcctg ccacaccgcg tgaaggatgc atttgcgaac gcctgccagc
     7981 gacctgtccg cgcgtccgag gccggtgagt tccgccggga gcgagtactg cgcgttcatg
     8041 ttctcgcagc ccccaccttt cagtatcagc ttcacttcga tctcgtcgtc gttctcccat
     8101 tgctcgaagt gaacgatcgg cgtgccgggg ccgaggttgt cgcccgagtt ctcgccggtg
     8161 agcgagtcca ccgagttcgg ccgcagcttg cctcgcctgg tggcctcggc caccgcgcga
     8221 cgaatctgct ctttcagcac gatctgattc acacccaccg gcaccttgac ctcgaaggtg
     8281 ggcatgcccg tgtcctggca gatggcgccc tcgcagtcgg aggccaggtc gatgttctgg
     8341 gcgatgacgg cgagcgcttg agcggactgt gtcccggcct gctcctgcgc catggcgact
     8401 ttcatcgccg cgcgcacgtc gggcgggaga tcggtggacg tccgcacgac gaggctgagc
     8461 acgctgtcga aaaacgaggg gagcatccgg ccagtataat ggggtcggga gtcatttctt
     8521 gaggacgctc cctcagtccc atacgcgctt cgcggggagt gtgctcaaga aatgactccc
     8581 gaccccattt ccccgacccg cgtcgagcac gattctctcg gcgagtacgc cgttccctcc
     8641 gacgcctatt acggcatcca gaccgcgagg gcggtcgaga actttccgat cagcggtctt
     8701 cgcgcccccg ccgacctggt caccgccacc gttgtcatca agggggccgc cgcgcgcgcc
     8761 aacgtcgagc tcgggcgcct cgcccgcggg gtcggcgacg cgatcgtcaa ggcggcggac
     8821 gagatcgccg gcggcgcgct gcgcgaacag ttcgtcgtgg acgtgtatca ggccggcgcc
     8881 gggacgtcgc atcacatgaa cgtcaacgag gtcctcgcca atcgggccgc ggagctgctg
     8941 ggcggctcga agggcgaata ccgtctcgtg caccccaacg atcacgtcaa catgggacag
     9001 tcaaccaacg acgtgttccc gacggccacg cgcctggcgc tgcttctcgg gcatcggccg
     9061 ctcgtcgacg ccgcgctcgc gctggcggcg tccttgcacc gcaaggcgat ggagttcgac
     9121 ggcgtcctca aagtcggccg gacgcacctt caggacgccg tgccaatgac gctcgggcag
     9181 gaattcggag gctacgccgc gtgcgtgcag cggggtgcgg aagacgtgga acgggcggcg
     9241 gaacagctga aggagctcaa cctcggagcc acggcggtcg gtaccggcct gaacgccggc
     9301 gacgacttcg cgaggcgcgc cgtcgcccac atcgccgcga acgtcgggat ggccctggcg
     9361 ccagccgcca accggttccg cgtgacccag agcatgggcg acgtactcgg gtactcgagc
     9421 gcggtgcggc gtctcgcgat cgaggtgagc aaggtcgcga gcgatctgag gctgctcagc
     9481 atgggccctc gcgcgggcct cggcgagatc accctgcccg cggtgcagcc gggctcgtcg
     9541 atcatgc
//
LOCUS       DBNGSH010000209         5941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3582166, whole genome shotgun sequence.
ACCESSION   DBNGSH010000209 DBNGSH010000000
VERSION     DBNGSH010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3582166"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="AC16CY_04710"
     CDS             <1..520
                     /locus_tag="AC16CY_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3353113.1"
                     /translation="AKGRPIRKKGIAPSLEGTLVFPNIIGAANWFSPSYSPQTKLFYQ
                     AAREMGTVYFKGPAVYKPGTAFTGGGGRHINGDEASGAVRALDATTGKLKWEFPLLSP
                     GYTSLLSTAGGLVFGGTEEGNFFALDAETGAPLWDVQLGAAVRGLPISYAVDGKQYVA
                     IAAGFTLFVFAL"
     gene            888..1883
                     /locus_tag="AC16CY_04715"
     CDS             888..1883
                     /locus_tag="AC16CY_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353114.1"
                     /translation="MTVRALVAPAMAFVALLFAPLGAQSPPARSAAAGWHMPRTPDGH
                     PDLQGYWTNDTVTPLERPAEFGSKETLTPEEAAAYAKKRLDEFLAQPKDDIHYDDAVW
                     QGERYDKHTLVRTSLILDPKNGRLPPLTPAGAERAAARAKTGGAGGPSDSAQSRTLAE
                     RCISWGNVGPPMIPPTYYANLHILQSADHVVIRHELLDGLRLIALDGRPHPGPGVQKL
                     AGDSRGRWDGDTLVVDTTNFSDKTNFRGPPRTTRQDITTSTKLRVVERFTLVDRETIR
                     YQFTVTDPETWTSPWSGEVPIRRFAGPIYEFACHEGNYGLVNILRGARVQESATR"
     gene            1893..2489
                     /gene="folE"
                     /locus_tag="AC16CY_04720"
     CDS             1893..2489
                     /gene="folE"
                     /locus_tag="AC16CY_04720"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR3353115.1"
                     /translation="MQDLIRQLLAELGEDPTREGLLRTPHRVEKALKFLTSGYDADID
                     EVLNDALFTVDYSEMVIVKDIDFYSLCEHHLLPFFGKCHVAYIPQRHVIGLSKIPRLV
                     DVFARRLQVQERMTNQIAETIREKIDPLGVAVVCEGTHLCMSMRGVEKQNSFAITSAM
                     LGVFRDNARTRMEFLELVRGRASEAAAQSAAGLACMGD"
     gene            2489..3178
                     /locus_tag="AC16CY_04725"
     CDS             2489..3178
                     /locus_tag="AC16CY_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353116.1"
                     /translation="MDVRGRIAFVALAAVLAGHVIVSAQTTQKPRSTAKPKPAPLPAL
                     KTEAATVTCPTLLGTGAKTGLMFCDVLIGGSDPASGVIVALPPHTGAVTLSFDLHNRH
                     TYSEEQVRSKRAYHRYTASIGVFALDNTLLGRAVVQSEFRTAADLVDRVLGGSGPGGL
                     KAVAPTGTESVVMTIEEMAESVAIVGETLSVVRLDGKDDFKTAGRPIAVISNVMLEYR
                     PGPPPRPARGR"
     gene            complement(3207..5330)
                     /locus_tag="AC16CY_04730"
     CDS             complement(3207..5330)
                     /locus_tag="AC16CY_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353117.1"
                     /translation="MPLILAIVADHHQAAQITALIKRLNVDLVQAAEVGEGLLALNDR
                     IPDLVLTSPLMSPFDDGVLDEYLRDLGSAGAHVQSLRIPVLSTSTKKSKRGGFSLFGR
                     AKETHTLEGCDPKVFADEITQYLTRAAEEKQNADENQAQGGLPESVLPGDDWSTTPGP
                     SPVVEAEEKWDPLAWQADAGSVEADSPAPLYNPIESVPAFSSVEGDSPYPSFEGGETT
                     TPEELQAADAPAADIYVPEPAIEAPPPLIVPEAEPPAVPAFDATAYTPIEEEPAAISA
                     IVEDPTPASSPDPDEPVVRAPLSLFTGVEATTDPTEYLRSMAAEPIVPEPIVQAPIVP
                     EPIVRAPIVLESIVRAPIVAEPTVPEPLVAEPMAAEEIVAEPIAAEEIVAEPMAAEPL
                     VAEPIVAAPPEPEATSVPLPDVPSIEDIEAAPAPEPIAPVVAAAIPDPPAAPPESPPP
                     VAEKHAPKLTASFRSALAAIRAAWDAPAKPKPPVVEKPTETMAPAKAEAPIPVKVASQ
                     GILGLRETIPIEATPASESMPAIEAPPAEPAAAFGVDSTGNAELIDGEAATVQPREPA
                     VDVGRLAEPEGEASEVYELTVEPDLQELESKLLAPLAPRVARPSPSEEPLGHASETRS
                     GEGRGTKDAPRAKRGKSKKGNKAAAAKPRRQDKGPVQDEWGIFDPDRCGFAALVDKLE
                     EVSEEKEQAEQAPRKGTKVRVISYS"
     gene            complement(5349..5624)
                     /locus_tag="AC16CY_04735"
     CDS             complement(5349..5624)
                     /locus_tag="AC16CY_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353118.1"
                     /translation="MRRVINDALISVGALVVLLVGLVSVDDRMRERLGAALSAPPNSA
                     QIINAGDRIGEVSRVMFIALRDQSVEHAPLVIFAVAATVLVLFMVRT"
BASE COUNT          978 a         1995 c         1947 g         1021 t
ORIGIN      
        1 cgccaagggg cgtccgattc gaaagaaagg gatcgcgccg agcctcgagg ggacgctcgt
       61 cttcccgaac atcatcggcg ccgccaactg gttcagcccg agctacagcc cgcagacgaa
      121 gctgttctac caggcggctc gcgaaatggg cacggtctac ttcaagggcc ctgccgtcta
      181 caagcccggc accgcgttca ccggcggggg aggacggcac atcaacggcg acgaggcctc
      241 gggcgccgta cgagcgctcg acgcgaccac tggaaaactg aagtgggagt ttccgctgct
      301 ctcccccgga tacacgagcc tgctgtccac ggctggtggc ctcgtcttcg gcggcacgga
      361 ggaagggaac ttcttcgcgc tcgacgccga gacgggcgca ccgctctggg acgtgcagct
      421 cggcgccgcg gtgcgcggcc tcccgatttc ctatgcggtc gacggcaaac agtacgtggc
      481 gattgccgcg ggcttcacgc tcttcgtgtt tgcgctctag cctcgaccac gaggccgtga
      541 tccaagcgcg gtacgtcggc ccggacgcgg cgcgggaccg gtcgctccgc aaaaccgact
      601 tgatccacgc ttccgcagct tcgccatcca tcaacgagct atgccaatac actcgccacg
      661 gtttttgctt cggacctacg gtcccgcacc gctgccagct ccggaggcgc gttcttccgc
      721 gccgaatcgg ggtccccgcc gcgcgtgttg tgcgcggtgg ggtgaaagtc gcgggctcgc
      781 agatactcga ctcaattgag cttcggacca ggtgttggaa gttgggagtt ggaggttgaa
      841 tgttgaagcc gtgtactatt ggcaatcaac tgcaggaggg aagggcgatg acagttcgag
      901 ccctggttgc gccagcgatg gccttcgtcg cgctgctgtt cgcgccgctc ggcgcgcaga
      961 gtccgccggc acgatcggcc gcggcgggtt ggcacatgcc acggacgccc gacgggcatc
     1021 cggacctgca ggggtattgg accaacgaca ccgtcacacc cctcgagcgc cccgcggagt
     1081 tcggcagcaa ggaaacgctc acccccgagg aagccgccgc gtacgcaaag aagcgcctcg
     1141 acgagttcct cgcgcagccg aaggacgaca ttcattacga cgatgcggtc tggcagggtg
     1201 agcggtacga caaacacacg ttggtccgca cgtcgttgat ccttgacccg aagaacggca
     1261 ggctccctcc gctgacgccc gcaggcgcgg agcgagcggc ggcccgcgcg aagaccggag
     1321 gcgccggggg cccgtccgac agcgcgcaga gccggacgct tgccgagcgg tgcatctcgt
     1381 ggggcaacgt cggtcccccg atgattccgc ccacctacta cgcgaacctg cacattctgc
     1441 agtcagccga tcacgtcgtg attcggcacg agttgctcga cggcctccgg ctgatcgcgc
     1501 tcgacggacg gccgcatccg gggcccggcg tacagaagct cgccggcgac tcgcgcggcc
     1561 gatgggacgg tgacacgctc gtggtggaca ccacgaactt cagcgacaag accaatttcc
     1621 gcggacctcc gcggacgacc cgccaggaca tcacgaccag cacgaagctg cgggtcgtcg
     1681 agcgcttcac gctcgtcgat cgggagacga tccgctacca gttcaccgtc accgatcccg
     1741 agacctggac gtcgccctgg tccggagagg tgcccatccg ccggttcgcg ggaccgatct
     1801 acgagttcgc ctgccacgag ggcaactacg gcctggtgaa catcctgcgg ggtgcgcggg
     1861 ttcaggaatc ggcgacacgt taagataact gcatgcagga tctcatccgc cagctcctgg
     1921 ctgagctcgg cgaagacccg actcgcgagg ggctgctccg gacgccgcac cgggtcgaga
     1981 aggctctcaa gttcctgacg agcggctacg acgccgacat cgacgaggtg ctcaacgacg
     2041 cgctcttcac ggtggactac agcgagatgg tcatcgtgaa ggacatcgat ttctacagcc
     2101 tctgcgagca ccacctgctt ccgttcttcg gcaagtgcca cgtcgcctac atcccgcagc
     2161 ggcacgtgat cggcctcagc aagattcccc ggctcgtcga cgtgttcgcc cgccgcctcc
     2221 aggtgcagga gcggatgacg aatcagatcg cggagacgat tcgcgaaaag atcgacccgc
     2281 tcggcgtcgc cgtcgtgtgc gagggcacgc acctgtgcat gtcgatgcgc ggggtcgaga
     2341 agcagaattc gttcgcgatc acgagcgcga tgctgggtgt cttcagggac aacgcccgca
     2401 cgcgcatgga gttcctcgag ctggtcaggg gccgggcctc cgaagccgcc gcgcagagcg
     2461 ccgcgggcct ggcctgcatg ggcgactgat ggacgtgcgg gggcgcatcg cgttcgtggc
     2521 gctcgccgcc gtgctggccg gtcacgtcat cgtatctgcc cagacgacgc agaagccgcg
     2581 atccacggcc aagcccaaac ccgcccctct tccagcgctc aagacggaag ccgctacggt
     2641 gacctgtccg acgctgctcg gcacgggcgc gaagacgggg ctgatgttct gcgacgtctt
     2701 gattggcggc tccgacccgg ccagcggggt catcgtcgcg ttgccgccgc acaccggggc
     2761 ggtgacgctg tcgttcgacc ttcacaatcg ccacacgtac tcggaggagc aagtccgcag
     2821 caagcgcgcg taccatcgct acacggcttc aatcggcgtg ttcgcgctcg acaacaccct
     2881 gctcgggcgc gccgtcgtcc agagcgagtt ccggaccgcc gctgacctgg tggaccgcgt
     2941 gcttggcggg agcggtcccg gcgggttgaa ggcggttgcc ccgacaggca ccgagtccgt
     3001 cgtcatgacg atcgaagaga tggccgagag cgtggccatc gtcggcgaga cgctgtccgt
     3061 cgtccggctc gacgggaaag acgacttcaa gacggcgggc cgtccgatcg ccgtgatcag
     3121 caacgtgatg ctggagtatc gtccggggcc tccgccgcgc ccggcgcgtg gtcgttgacg
     3181 cgccagtcct cgccccggga acacaatcag ctgtaggaga tgacccgcac cttcgtgcct
     3241 ttgcggggcg cctgctccgc ctgttccttc tcttcggaca cttcttccag cttgtcgacg
     3301 agcgcggcaa atccgcatcg atcgggatcg aaaatgcccc attcgtcctg gacgggccct
     3361 ttgtcttgac ggcggggctt tgccgctgcg gctttgttgc ccttcttgga tttcccgcgc
     3421 ttggcgcgcg gtgcgtcctt ggtgccgcgg ccctcgccag accgagtctc gctcgcgtga
     3481 ccgaggggtt cttccgaagg cgacggcctg gccacacgcg gcgcgagcgg cgcgagcagc
     3541 ttcgattcca gttcctgcag gtccggctcc accgtgagtt cgtagacttc cgacgcctcg
     3601 ccctcaggtt cagccagacg tccaacgtcg acggccggtt cccgcggctg cacagtggcc
     3661 gcctcgccgt cgatcagttc cgcgttgccg gtcgaatcga caccgaacgc cgccgccggc
     3721 tccgcaggcg gcgcctcgat cgcaggcatc gactcgcttg caggcgtcgc ctcgattgga
     3781 atcgtctctc ggagccccag tattccctgg ctggcaacct tgaccggtat cggggcctcg
     3841 gccttggctg gagccatcgt ctcggtcggc ttttccacga ccggaggctt cggcttcgcg
     3901 ggcgcgtccc acgccgctcg aatcgcggcc agcgctgacc ggaatgaggc cgtcagcttg
     3961 ggggcgtgct tctccgccac cggcgggggt gactccggcg gggcagcagg tggatccgga
     4021 atcgcggcgg cgaccaccgg cgctatcggc tccggggctg gcgccgcctc gatatcctcg
     4081 atcgagggaa catccggcag cggcacgctc gtcgcctccg gctccggcgg cgcggcgaca
     4141 atcggctcgg ccacgagtgg ctccgcggcc attggttccg cgacgatctc ttccgcggct
     4201 attggttctg cgacgatctc ttccgcggcc attggttccg cgacaagtgg ttccggcacg
     4261 gtcggctcag cgacgatcgg cgcccggacg atcgactcca ggacgatcgg cgcccggacg
     4321 atcggctccg ggacgatcgg cgcctgaacg atcggctccg ggacgatcgg ctcggcagcc
     4381 atcgaccgga ggtactcagt cggatccgtc gttgcttcga ctcctgtgaa cagcgacaac
     4441 ggcgcccgta cgactggctc atccggatcg ggagacgaag ccggagtcgg atcttcgacg
     4501 atcgccgaga tcgcggccgg ctcctcctcg atgggtgtat acgccgtcgc gtcgaaggcg
     4561 ggcaccgcgg gaggctcagc ctccggcaca atcaacggcg gcggggcctc gatcgccggc
     4621 tcgggaacat agatatcggc tgcgggcgcg tcggccgcct ggagttcttc cggcgtagtc
     4681 gtttccccgc cttcgaaaga agggtagggc gagtcgccct caaccgaact gaacgcaggc
     4741 acgctctcga tggggttgta cagcggcgcc ggcgaatccg cctctacact gcctgcgtcg
     4801 gcctgccagg ccagcggatc ccacttctcc tcagcctcga cgaccgggct ggggcccggc
     4861 gttgtcgacc agtcatctcc gggtaaaacg gattcgggca gaccgccctg tgcctgattc
     4921 tcgtcggcgt tctgcttttc ctccgccgcc cgagtcaggt actgggtgat ctcatcggcg
     4981 aaaaccttcg gatcgcatcc ctcgagcgta tgggtttctt tcgctcggcc gaacagcgag
     5041 aacccgcccc gtttcgactt cttcgtggac gtgctcagca ccgggatacg cagcgactgg
     5101 acgtgagcgc ccgccgagcc caggtcgcga aggtactcgt ccagcacgcc gtcgtcgaac
     5161 ggcgacatca gcggcgacgt caggacgaga tccggaatgc ggtcgttgag cgccaggagt
     5221 ccctcgccga cttccgcggc ctggaccagg tcgacgttga ggcgcttgat taacgccgtg
     5281 atctgtgcgg cctggtggtg atcggcaacg atggcgagga tcagtggcat gtcgtgggcg
     5341 tccgcccgtc aggttcggac catgaacaat acgagcaccg tcgccgccac cgcgaagatc
     5401 accaggggcg cgtgctcgac gctctgatcc cgcagcgcga taaacatgac gcgggacacc
     5461 tcaccgattc gatccccggc gttgataatc tgggcggagt tcgggggagc gctcagcgcg
     5521 gccccaagac gctcgcgcat acggtcgtcg accgacacga gaccgacgag cagcacgacg
     5581 agtgcaccca ctgagatcag cgcgtcgttg attacacgtc gcatcgttct atttccttga
     5641 tggcgacact ccgtcggcaa agcgtacgca agcgtcgttc cggattcgtt cgtgggcctc
     5701 aagaagctgt cactgttcga gttgaagtgc gcagcagggg ggctgaaacg ctcgcgattc
     5761 acagaattcg tgggtcgatt tacacatccg ccgtgcacgc gcgagctgac gccgcgctga
     5821 cgccgatgac gcggtgtgca gtctcgtcct gtgtgtcgat cccgcgtgcg cttcgtgtcg
     5881 atccgggaca cgccgctgga gccacggaat cgaccggttc gttacgaacc tgacgactcg
     5941 a
//
LOCUS       DBNGSH010000210         1842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1544914, whole genome shotgun sequence.
ACCESSION   DBNGSH010000210 DBNGSH010000000
VERSION     DBNGSH010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1544914"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="AC16CY_04740"
     CDS             complement(<1..374)
                     /locus_tag="AC16CY_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HIR3353119.1"
                     /translation="MTDTGARFERLVEIMRRLRAPDGCPWDREQTPHSLRPFVLEETY
                     EVLEAIESGIPAHLCEELGDFLFEAVFLAQMSEEAGHFGIGDAIDAICDKLVRRHPHV
                     FARGEGESPLTSRQVIEKWETL"
     gene            408..1376
                     /locus_tag="AC16CY_04745"
     CDS             408..1376
                     /locus_tag="AC16CY_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353120.1"
                     /translation="MLVILGVCVVWAVNLHAQNAGPHPRTPWGDPDLQGYYTNKYEYG
                     TPFERPAAFDGRRVNEVSGKELADLAAKRQQEALDRAPFFGGDPAGKIGNSAEFRDIY
                     EVTRGSRAWLVTDPPDGKIPPIAPQARARVQDTTRGGSFGSGPFDGPEDFSLWERCIT
                     RGLPGSMLPGGYGNSYQIVQGPGFVAIRYEMVHETRIIPLDDRPPLPGTLRFDMGSPR
                     GRWEGETLVVETTNFLQRSAYRNASADTLRLVERFRRTSPTHVEWSVTVIDPSTWTRP
                     WTFSMPLTRNDGERIEMYECHEGNHAIFNILSAARAAEREAARRSR"
     gene            1384..1689
                     /locus_tag="AC16CY_04750"
     CDS             1384..1689
                     /locus_tag="AC16CY_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HIR3353121.1"
                     /translation="MSEHTQETGEITFIGFVLSLAHTAAYHFGDVHDPVSGKAGEANL
                     PAAHQILEILALLEEKTRGNLTAEERQLLEQILYELRMRYVEASKAPPQEPSRIIIP"
     gene            1736..>1842
                     /locus_tag="AC16CY_04755"
     CDS             1736..>1842
                     /locus_tag="AC16CY_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004873091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353122.1"
                     /translation="MSRQVQARVVFLGTGTSHGVPMIGCDCAVCRSEDPR"
BASE COUNT          308 a          627 c          609 g          298 t
ORIGIN      
        1 ttgagcgtct cccacttctc gatcacctga cggctcgtca gcggggactc gccctccccg
       61 cgcgcgaaga cgtgcggatg gcgccggacc agcttgtcgc agatggcgtc gatcgcgtcg
      121 ccgatgccga agtggcccgc ctcctcgctc atctgggcca ggaacacggc ctcgaacagg
      181 aagtcgccca gctcctcgca caggtgggcg ggtatgccgc tctcgatcgc ctccagcact
      241 tcgtaggttt cctcgaggac gaacggacgc agcgagtgcg gcgtctgctc gcgatcccac
      301 gggcagccgt ccggtgcccg cagcctgcgc atgatctcca cgagtcgttc gaagcgggct
      361 cctgtgtccg tcacggcgtc atgctaccat ccaccagaac gatgcgtttg ctcgtgatcc
      421 tgggcgtgtg cgtcgtgtgg gccgtgaacc tgcacgcaca gaatgcgggt cctcacccgc
      481 ggacgccgtg gggcgatccc gatctgcagg gctattacac gaacaagtac gaatacggca
      541 cgccgttcga gcggccggcg gccttcgacg gtcgtcgcgt caacgaagtg tccgggaaag
      601 agctcgccga tctcgccgcg aagcggcagc aggaagcgct cgaccgcgcg ccgttctttg
      661 gtggcgaccc ggccggcaag atcggcaatt ccgccgagtt tcgagacatc tacgaagtga
      721 cgcgcggcag ccgcgcatgg ctcgtcaccg acccgcccga cgggaagatt ccgccgatcg
      781 cgccgcaggc gcgcgcgcgt gtgcaggaca cgacccgcgg cggcagcttc ggctcggggc
      841 ccttcgacgg ccctgaagat ttcagcttgt gggagcgttg catcacgcga gggcttcccg
      901 gctccatgct gcccggcggc tacggcaact cgtatcagat cgtgcagggt ccagggttcg
      961 tcgccatccg ctacgagatg gtgcacgaga cgcgcatcat cccgctcgac gatcgcccac
     1021 ccctgcctgg aacgctccgg ttcgacatgg ggagcccccg cggccgctgg gaaggggaga
     1081 cgctcgtcgt cgagaccacg aactttcttc agcgcagcgc gtaccgcaac gcgagcgccg
     1141 acacgctgcg cctggtcgag cgcttcagac gcacgtcgcc gacccatgtg gaatggtccg
     1201 tcacggtgat cgatccctcg acgtggacgc ggccctggac cttctcgatg ccgctgaccc
     1261 gcaacgacgg ggaacgcatc gagatgtacg agtgtcacga ggggaatcac gccatcttca
     1321 acatcctcag cgcggcgcgt gccgctgagc gcgaggcagc ccggcggtca cggtaaagcg
     1381 cccatgtccg aacacacgca ggagaccgga gagatcacct tcatcggctt cgtgctgtcg
     1441 ctcgcgcaca cggccgcgta ccacttcgga gacgtgcacg acccggtctc gggtaaggcc
     1501 ggcgaggcga acctgccggc cgcgcaccag atcctcgaga tcctggcgct gctggaggag
     1561 aagacccgcg gcaacctgac ggccgaggag cggcagttgc tggagcagat tctctacgag
     1621 ctgcgcatgc gctacgtgga ggcttcgaag gccccgccgc aggagccctc gcgcatcatc
     1681 atcccttgac ccttcgattg gcactcaggg tcaccccgag cggagtcgag gggtgatgtc
     1741 tcggcaggtc caggcccggg tggtcttcct cggcacgggt acgtcgcacg gcgtgccgat
     1801 gattgggtgc gactgcgcgg tgtgccgatc ggaagacccg cg
//
LOCUS       DBNGSH010000211         3417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_526203, whole genome shotgun sequence.
ACCESSION   DBNGSH010000211 DBNGSH010000000
VERSION     DBNGSH010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_526203"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="AC16CY_04760"
     CDS             complement(<1..555)
                     /locus_tag="AC16CY_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3353123.1"
                     /translation="MSRPGLVLVTLCVAATVYAARPTFWEVSTEPEFLRGEIENLAVD
                     TFGRLTLGPTSAPVYETTAPFVWTLLGTPDGTMYAGSGNDGQVYRIDPSGKGSVFFDA
                     DELEVHALALVPGGGLYVGTSPNGKIYKVEASGASAVFFDPPDRYIWSLVVDGAGNVF
                     AATGDQGKIYKIAPNGTGSEFYATK"
     gene            complement(618..2486)
                     /locus_tag="AC16CY_04765"
     CDS             complement(618..2486)
                     /locus_tag="AC16CY_04765"
                     /inference="COORDINATES: protein motif:HMM:NF017400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HIR3353124.1"
                     /translation="MRIRLIGLAVLLVSAMIAGLPAQTATFPVSEVKAGMVGIGRTVF
                     QGEEIEQFRAHILGVLHNSIGPRRDLILARLEGGPLANTGVLAGMSGSPVYIDGRLLG
                     AVSYSLGEFSKEPLAGITPFEEMLADATLPGQRRPLARASLTLPVTHDGLRDSLRQAF
                     SWARPFADSAADVRVIGAPLNAGIGTLLRPIATPINFGGFEQSAIDPLVSAFRDLGFT
                     PVVAGGGGQLGSTLSPAPPTPSAAASSGRALRPGDPIGVALMNGDLEVGATGTVTEVS
                     GDRVYAFGHPFYGLGPTQFPMTRARVLAVLPSLSSSMKIASTGEVIGIVQQDRATTIA
                     GTLGPGPAMIPITLTLSAERGTRRQFTMSMVNDQLLTPLLAYVAILNTLTSYERQNGV
                     GTYSVRGAARVKNHADVAFEDLFTGDQPSVGAAASVVAPINLLLRNAFEDVQFEGLSL
                     DIEASEQPRSATIERVWVDGARVRAGTSVDLRILLRTYRGEEVTRSLPIDIPANAKTN
                     VTIMVTDAARLSQWEAREFQVQPMQSTGLPQMIRVLNNARKNNRLYVRLITRDGGALV
                     KGEPLAALPSSVLAVMESERNGGSFRPLQSALTGEWELTAGYAVTGARTLTVPLEE"
     gene            2764..>3417
                     /locus_tag="AC16CY_04770"
     CDS             2764..>3417
                     /locus_tag="AC16CY_04770"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR3353125.1"
                     /translation="MSLLCETVTGRSMADLMRARDAVSLADMVELRFDGVIDLDVAQA
                     LHGRRLPVVATCRPRWEGGRFDGSEDERGRILKQALDLGAEHVDLEWRAIEGNASHAT
                     FGDLIRRDPARVVVSSHEFESLPGDLEDRVRAMRATGAGTIKVAATVSRLEETLRLKT
                     IAHDGPTVAVAMGEVGVPSRLLAARFGSCWTYAGDGVAPGQVPAARMIEDYAFRRVGT
                     "
BASE COUNT          528 a         1112 c         1194 g          583 t
ORIGIN      
        1 cttggtcgcg tagaactcgc tccccgtgcc gttcggcgcg atcttgtaga tcttgccctg
       61 gtctccggtc gcggcgaaga cgttgccggc accgtcaacg acgaggctcc agatgtacct
      121 gtccggcgga tcgaagaaga ccgcgctcgc gccgctcgcc tcgaccttgt agatcttgcc
      181 gttcggtgag gtgcccacgt agagcccgcc gccgggaacc agcgcaagcg cgtggacctc
      241 gagttcgtcc gcgtcgaaga acaccgagcc tttgccggac gggtcgatgc gatacacctg
      301 gccgtcgttg ccgctgccgg cgtacatggt tccgtcgggt gttcccagca gcgtccagac
      361 gaacggcgcg gtcgtttcgt acaccggcgc ggacgtaggg ccgagggtga gccgaccgaa
      421 ggtgtctacg gccaggttct cgatctcgcc gcgcaggaat tccggctctg tcgagacttc
      481 ccagaacgtg ggcctcgccg cgtacaccgt cgcggcgacg cacaacgtga cgagcacgag
      541 tccaggtcga gacacgttca ggttgcgacc tttgagtctt gagtttagag ctttgagttt
      601 tgagtcttgc cttacagtca ttcttccagt ggcaccgtca gcgtccgcgc tcccgtgacg
      661 gcgtaacccg cggtgagctc ccattcgccc gtcagcgcgc tctgcagcgg gcggaagctt
      721 ccgccgttgc gttccgactc catcacggca agcacggacg aggggagcgc cgcgagcggc
      781 tctcccttga cgagggctcc gccgtcgcgc gtgatgagcc ggacgtagag ccggttgttc
      841 ttgcgcgcgt tgttgagcac gcgaatcatc tgcggcagtc cggtcgactg cattggttga
      901 acctggaact cgcgcgcctc ccactgcgac agccgcgccg catcggtgac catgatcgtc
      961 acgttcgtct tcgcgttggc gggaatgtcg atcggaagcg agcgcgtcac ctcttcgccc
     1021 cggtatgtcc tgagcaggat gcggagatcc accgaggtgc cggccctgac gcgcgcgccg
     1081 tccacccaca cgcgctcgat ggtggcgctc cgcggctgct cgctggcttc gatgtcaagg
     1141 ctcaagcctt cgaactgcac atcctcgaac gcgtttctca gcaggaggtt gatcggcgcg
     1201 acgaccgacg cggccgcgcc caccgagggc tggtcgccgg tgaagaggtc ctcgaaggcg
     1261 acgtcggcgt ggttcttgac gcgggccgct ccccgcacgg agtaggtgcc gacgccgttc
     1321 tggcgctcgt aggaggtcag cgtgttgaga atcgcgacgt aggcgagcag cggcgtcagc
     1381 agctgatcgt tgaccatgct catcgtgaac tgcctgcgcg tgccgcgctc ggcgctgagg
     1441 gtcagcgtga tcggaatcat cgcgggtccc ggtccgagcg tgccggcgat ggtcgtggcc
     1501 cggtcctgct ggacgatgcc gatgacctcg ccggtgcttg cgatcttcat cgagctcgac
     1561 aggcttggca gcaccgccag gacccgggcg cgcgtcatcg gaaactgcgt cggcccgagg
     1621 ccgtagaacg gatggccgaa cgcatagacg cggtctccgc tgacctcggt gaccgtgccg
     1681 gtcgcgccca cctccaggtc gccgttcatc agcgcgacgc cgatcggatc gcccggccgc
     1741 agcgcgcgtc ccgaagatgc cgccgcggac ggagtcggcg gggcgggaga gagggtggaa
     1801 ccgagctggc cgccgccgcc ggcgaccacg ggggtgaatc ccagatcgcg gaacgccgag
     1861 acgaggggat cgatcgcgga ctgctcgaag ccgccgaagt tgatcggcgt ggcgatcggc
     1921 cggagcagcg tgccgatgcc cgcgttcagc ggcgccccga tgacgcgcac gtcggccgcg
     1981 ctgtcggcaa aaggccgcgc ccacgagaag gcctgtctca gcgagtcgcg aaggccgtcg
     2041 tgcgtgaccg gcagcgtcag cgacgcgcgg gcgagcggcc gcctctggcc cggcagcgtg
     2101 gcatccgcga gcatctcctc gaacggcgtg atgcccgcga gcggctcctt cgagaattca
     2161 ccgagggaat acgacacggc gccgagcagg cggccgtcga tgtagaccgg gctgccgctc
     2221 atgcccgcga ggacgcccgt gttggcgaga ggaccgcctt cgagccgcgc gaggatcaga
     2281 tcgcgccgcg ggccgatgct gttgtgcagc acgccgagga tgtgtgcacg gaattgctcg
     2341 atttcctcgc cctgaaagac cgtccggccg atgccgacca tgcctgcctt gacctcgctg
     2401 acagggaagg tcgcggtttg cgccggcagc ccggcgatca tggccgaaac cagcagaacc
     2461 gcgaggccga tcaggcggat gcgcatcggg gggctcgtag ttgatgatac gggtaaacct
     2521 ctgaaatcgc tagggtccgg gcttcgcgcg gttgacagag gtgagcgctt gcgttactgt
     2581 cctttcaccg gtcagacatg acccgcacct ttagcccctt tgtgccgact gccgccagcg
     2641 cttactggtg gtggtacgga tctgcggctg agggggcgac tgtgcacgtc tgaagcgttc
     2701 gaacgaccga cagacattga cgccccttca gccaaggctg aaggggcttt ttttttggtg
     2761 accatgtcgc tgctctgcga aaccgtgacc ggccgatcga tggccgacct gatgcgcgcg
     2821 cgggacgccg tctcgctcgc cgacatggtg gagctccgtt tcgacggcgt gatcgacctc
     2881 gatgtcgccc aggccctgca cggacgccgc ctgccggtcg tggcgacgtg ccggccgcgc
     2941 tgggagggag gccgcttcga cggaagcgag gacgaacgcg ggcggatcct gaagcaggcc
     3001 ctcgatctcg gggcggagca cgtggatctg gagtggcggg cgatcgaggg aaacgcctcg
     3061 cacgcgacct ttggcgatct gattcgccgg gatccggcgc gcgtcgtcgt gtcgtcgcac
     3121 gagttcgagt ctctgccggg ggatctcgag gatcgggttc gcgcgatgcg ggccacgggg
     3181 gccggcacga tcaaggtggc cgccaccgtc tcgcggctgg aggaaacgct gcgcctcaaa
     3241 acgatcgcgc acgacggacc caccgtcgca gtggccatgg gcgaggtcgg cgttccttcg
     3301 aggctgctcg ccgcccgctt cggttcctgc tggacctacg ccggcgacgg tgtcgcgcca
     3361 gggcaggtcc ctgccgcacg gatgatcgag gactacgcgt tcaggcgcgt cgggacg
//
LOCUS       DBNGSH010000212        11643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1545009, whole genome shotgun sequence.
ACCESSION   DBNGSH010000212 DBNGSH010000000
VERSION     DBNGSH010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1545009"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1329)
                     /locus_tag="AC16CY_04775"
     CDS             complement(55..1329)
                     /locus_tag="AC16CY_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HIR3353126.1"
                     /translation="MTRILAIEPDPKRGVILRTLVRQRLTAEVVLATSTDAAIATMAE
                     KPPTLILMSSVIAPEEDLRLAARLRQAPALRHVPVLTVPFLLDTSNGTAADRQGLLSR
                     LIPQRGPSSAYDFGAVVARIEDAIEMARKEASLEAFGCVRPLLVDADSSLQAVDAPHR
                     LVRVSSGLDAFCGPRSKRSRARRLTRVDLPWFSSIKLTWGPELQLVNISRSGLLVETG
                     IRLIPGGNTAFEIAGPDKDLVVRARVIRTEVSAVDSIGEKYLAAAAFERSLDSLLPAG
                     SQLESPDPAALLAELTTRLRDAAARGAHPVELRADFEAAIQELVTGCEVRLREAPVAE
                     NNGRDAVYFTVPGNNRQPAVLQATFEPGRQPEPEEFEALKAASLAASDVLEFTEPARQ
                     KSLSTALERVGATAWRAAGGRVTSIRELRPTA"
     gene            complement(1528..2925)
                     /locus_tag="AC16CY_04780"
     CDS             complement(1528..2925)
                     /locus_tag="AC16CY_04780"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR3353127.1"
                     /translation="MSPNLVALSYLAASVLFILALKGLSNPLSARRGNRFGIAGMVLA
                     VLTTLAITRRVDLTLLALGAGGAIGWLVARRVQMTQMPELVAAMHSLVGLAAVLIAIA
                     VVNNPTAFGVGDPVPVGNRVELFIGTFVGAMTFSGSVIAFGKLAGLGRLTRLFSSAPV
                     VFRGQQVVNLTLAIAMIGLGVSFVLAPPGAQWLSFFAMTAVAFVLGVLVIIPIGGADM
                     PVVISMLNSYSGWAASGIGFSLNNPLLIVAGSLVGSSGAILSYIMCKAMNRSFFSVIL
                     GGFGAAEGAAAATEQKSFRSGSPEDVAFLLGNAESVIIVPGYGLAVARAQHAVKEMAQ
                     KLSAKGVTVRYAIHPVAGRMPGHMNVLLAEAEVPYDQVLEMDEINGEFGGTDIAFVLG
                     ANDVVNPLAEQRGSPIYGMPILEAHKARTVVVNKRSMASGYAGIDNPLFYMDKTMMVF
                     GDAKKVVEDIVKAIE"
     gene            complement(2928..3209)
                     /locus_tag="AC16CY_04785"
     CDS             complement(2928..3209)
                     /locus_tag="AC16CY_04785"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR3353128.1"
                     /translation="MNLMVFVLAVFVGYHVVWNVTPALHTPLMSVTNAISSVIVVGAI
                     LAAGSGRMDSGTMLGFIAVVLAAVNTFGGFLVTQRMLAMFKKKEPRAKP"
     gene            complement(3241..4356)
                     /locus_tag="AC16CY_04790"
     CDS             complement(3241..4356)
                     /locus_tag="AC16CY_04790"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR3353129.1"
                     /translation="MRIGIPAEVYPGETRVAATPETVKKLTAGGRHTVTVETSAGAGA
                     SIPDREFAAAGASLGCAADLYASSDIVLKVHRPTDTELPLLRRGSILVGLLTPHQDMT
                     ALAHTGVTAFAMELLPRITRAQNMDVLSSQANIAGYRAVLVAAHEYGRFMPMLMTAAG
                     TVKAARVLVLGAGVAGLQAIATAKRLGAVIEAFDVRPAVKEQVESLGARFVEVPLSEE
                     EKRAQDTAGGYAREMSDDYKRRQSALIAERAKAADIVITTALVPGRPAPVLLPEDTIA
                     GMKPGSVVVDLAVSQGGNCTQSVKDTVVVLNGVKIVAPSNLPASLAADASALYARNLL
                     NFTTLLLDAKTGELRVDRSDEIIAATLVCIDGAAVRS"
     gene            4484..5260
                     /locus_tag="AC16CY_04795"
     CDS             4484..5260
                     /locus_tag="AC16CY_04795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3353130.1"
                     /translation="MDLGLTDRVAIITGSSRGLGLASAKALISEGCCVTICGRTQATL
                     DDARRQLTELSGRDERVLTVAADVSRPDGAHAVVDRTIQAFGAVDVLVNNVGLARGSS
                     LLETSDGQWQEAIDQTLFPAIRVSRLAVPHMQRRGGGSIIMIASIYGREAGGRMTYNA
                     VKAAEISLAKSLAQQLAPMNIRVNSVAPGSILFEGGSWWKRQRDDPAAMADYVKRELP
                     FGRFGRAEEVGDVVAFLASRRSSWISGASVVVDGCQSRAF"
     gene            5277..7403
                     /locus_tag="AC16CY_04800"
     CDS             5277..7403
                     /locus_tag="AC16CY_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353131.1"
                     /translation="MTTETRSDASTVRAARPAFLTDIGIAVFGVLLFQLVIVSSSRLT
                     ANGGFGWDGESYAGMVANSLASGNPNTQMRPLLVLLTRVPYALGLDLYHSFVALDYVY
                     AFGLYLAASLLLAHAGLTWPVRALLVVNLALSIGTSKMYAFYPTLIDFGALALVTWSF
                     YLSTTDRHRLAGLVCILAAAAREFGVPAALYGMHRAFRQRGWANVAMYVPSLVTFALI
                     RWRVSAAFAGGPDQGPLDLQDAIDRLALWASPWFPVMFAYFTTSVFGGISALLVLRLR
                     WCIGRLRAEPELATLLALVGAGAVAAGGDVWRYLVFAMPAAVALAGRYLAGCEPDLRR
                     RGLIAVTAVTVITQRPFELMDRAKYFLDWFPLYYVLDLLEPIDTFVPVWAGRLLVMAL
                     LLVALYFAAVWTGRRSHVPAAGNGSPSSRGWTLDVLAVLALAAVASWPVQRELQAPEP
                     PTLEAGARDKVGWETENRRVLQGDVPLIESGRLAPQSQLARPQTLLLYPPHLVLRFVP
                     IALFFPVSLALHAWLAGLGGYMVARQLRVPRLASLAAGVAVMGGGVLMPVADPFSDGV
                     QQLAWMPLALALTMRSTARGGVWPHPALVAIVAIGLLSGSLRGAMYVIGTAGAWYLFD
                     VLVPVDSRPRHTAPARQFAMLCALGAGLCAVVLLPAFESALSSDGAGGVVSLVIGVGV
                     SASTASAWFSTEFADYGKFMRRTRSR"
     gene            complement(7367..9628)
                     /locus_tag="AC16CY_04805"
     CDS             complement(7367..9628)
                     /locus_tag="AC16CY_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3353132.1"
                     /translation="MKAHAPHQIGRYRVDARLGTGGMGTVYRAYDEKLRRDVAIKLLH
                     DESSGAGTRQLLHEARASSALNHPGICTVYEVEDDGQQAFIVMELVDGQRLSDLIPAG
                     GLPFESARAYGLQVADALAHAHARSVVHRDVKPANILVTRQGRTKVLDFGLARHVGAQ
                     VVERTTQTPSSFSDDLAGTLPYMAPEQLRGEPATARSDVWSLGVVLYELASGRRPHEG
                     DTPFTLSSSILGAAPLPLGEQVAPGLKKIVLRCLAKDPGERYQDAGQVHAALDALESP
                     RRSPGGRSGSRPSPRARIRSLAVLPLEDLSAESGEAFFADGMTDALITTLAQIGALRV
                     ISRTSVMRYKGTRLGLPEIARELNVDAVVEGTVLRSHGRIRIAAQLIHAATDTHLWAK
                     QYETDLRDVLALQSEVARAIADGIRVQLSPAEKTRLSGSRPVDPVAYEAFLKGRHLWY
                     KRSPDALKRGVESLQQAIDLDPTYALAHAGLADAYVSLGWDLFGLALPSEAYPKAKQA
                     ALRALELDPNCAEAHAALGWTAGGFDWDWAAAERGLRHAIALKPQYGPVHIWYSHLLR
                     ALNRTEESLAESRRALECDPLGLVLNMHLGWHHLYSRQYQKAVEQCQKTLELDHTFIL
                     AHVFLGQAYEQLGERSSAIQEFEVAAELSQRHPAYLADLGHAYAAAGRRADAQAVLDE
                     MTRVASQRYVAARAVAEVHIGLGDLDQAFAWLDKAFEQRNGWLIHIRENPRYDRLRPD
                     ARYLDLVRRMNFP"
     gene            9694..10377
                     /locus_tag="AC16CY_04810"
     CDS             9694..10377
                     /locus_tag="AC16CY_04810"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR3353133.1"
                     /translation="MSRRLLDAQLSPYLDGLVPPRPPEMAAMEAEAAKTDFPIIGPTA
                     GHVCYLIARLIGARSVFELGSGFGYSTAWFARAVRDNGGGVVHHTVWDDELSRRARGH
                     LSALGYADLVTFHVGESVAALRESTGPFDLIFNDIDKAGYPASLPVIASRLRPGGVLI
                     VDNMLWSGRIFDEADRSAHTQGVRTLTQLVTADPDWTASIIPVRDGLLVAQRNPRGRA
                     GAPVRPERT"
     gene            complement(10334..10681)
                     /locus_tag="AC16CY_04815"
     CDS             complement(10334..10681)
                     /locus_tag="AC16CY_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353134.1"
                     /translation="MSADRYTKIVLTVIAVELGWLGVKDAAVPVSAQQVEPMPVIIKG
                     VEGVRGRPLVFPVRIAEATAPVRVTVDRPLPIEAPLPIRIDAINPVVIETSPGRPLLV
                     KSVQAEPAPRPGL"
     gene            complement(10737..11492)
                     /locus_tag="AC16CY_04820"
     CDS             complement(10737..11492)
                     /locus_tag="AC16CY_04820"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3353135.1"
                     /translation="MPPPTFRARLRSGERVIAPLVTLNSPPVVELLAEAGFDWLFLDA
                     EHAPFDALEMQALMQAAGATPCVVRLASSDEIHVKQALDIGAAGIIAPQINSAEHARQ
                     IVQWSKYAPAGRRGMGIARAHRYGLKVPEYLHTANEDTAVIVQAEHHEAVKHIREIVR
                     VEGVDGVLIGPYDLSASLGIPGRVDHQDVKDAIARVSDACREAHLPIGIFGLTADAVR
                     PYIEQGFTMIVAGVDTVMLAQASTALLKSVKAI"
BASE COUNT         1823 a         3876 c         3894 g         2050 t
ORIGIN      
        1 cggagaaccc ggcggtgctg atccgcgact tcaagaccga caaaacctac atcatcacgc
       61 ggtcggacgt aattcgcgta ttgatgtgac ccgccctccg gcggcgcgcc acgccgttgc
      121 gccgacgcgc tccagcgcag tcgagaggct cttctgtctg gctggctcgg tgaattccaa
      181 gacgtcggac gcggcgagcg acgcggcctt gagcgcctcg aattcctccg gctcgggctg
      241 gcggccgggc tcgaaggtcg cctggagcac ggccggctga cgattgttgc ccgggacggt
      301 gaaatacacg gcgtcgcggc cgttgttttc cgccacgggc gcctcgcgga gacgaacctc
      361 gcagcccgtg acgagctctt gaatcgccgc ctcgaaatca gcgcggagtt cgacgggatg
      421 ggccccgcgc gccgcggcgt cccgcaaccg agtcgtgagc tccgccagca gcgccgccgg
      481 atcgggcgat tcgagctggc tgcctgcggg cagcagtgag tccaacgacc gctcgaaggc
      541 cgcggccgcc aggtatttct cgccaatcga atccacggcg gacacttcgg ttctgatcac
      601 gcgtgcccgc acgactaagt ccttgtccgg cccggcgatc tcgaaggccg tgttgccgcc
      661 gggtatcagg cggatgcccg tttccaccag cagcccactg cgcgaaatat tcacgagttg
      721 cagctccggg ccccacgtga gcttgatgct ggagaaccac ggcagatcga ctctcgtcag
      781 ccgccgggcg cggctcctct tcgaccgggg cccacagaag gcgtcgagcc ccgagctcac
      841 gcgcaccagg cgatgcggtg cgtccacggc ctggagcgat gaatcagcgt caacgagcag
      901 cggccggacg catccgaagg cttcgagtga ggcctctttc cgcgccatct cgatggcgtc
      961 ctcgatcctg gccaccacgg ccccgaaatc gtacgcgctt gacgggccgc gctgggggat
     1021 gagacgagac agcagtccct gccggtccgc ggcggtgccg ttgctggtgt cgaggaggaa
     1081 cggaacagtg aggactggta catgccgaag tgcgggtgcc tgtctcaagc gcgcggcaag
     1141 gcgaagatcc tcctctggcg cgatgacgct cgacatcaga atgagcgtgg gcggcttctc
     1201 ggccatcgtc gcgatggccg cgtcggttga ggtcgccaga acgacttcag ccgtcaatcg
     1261 ctgtcggacc agcgtccgca ggatgacgcc ccttttggga tccggctcga tcgcgaggat
     1321 tcgtgtcatg ggtccgcccg cctggagacg tcaggggcaa tcggcggacc gccgcgattc
     1381 cgagcaaaac tcgcgatatt ggcgggtttt cagcgcgcgg caggctgacg tcttccagga
     1441 ggcagactaa gacgtggtga actcagaatg tctccgtgcg cgctctgcac acgcctgcat
     1501 agtccggcta cagccggaca ctgcgtatta ttcaatcgcc ttgacgatgt cttcgacgac
     1561 cttctttgcg tcgccgaaca ccatcatcgt cttgtccatg tagaagaggg ggttgtcgat
     1621 ccctgcgtag cccgatgcca tcgaccgctt gttgaccacg accgtccggg ccttgtgcgc
     1681 ttcgagaatg ggcatgccgt agatcggact gccgcgctgc tccgcgagcg gattgaccac
     1741 gtcgttggcg ccgagcacga acgcgatgtc ggtgccgccg aactcaccgt tgatctcgtc
     1801 catttcgagc acctgatcgt aggggacctc ggcttcggcg agcaggacgt tcatgtgtcc
     1861 cggcatgcgg ccggcgaccg ggtgaatggc gtagcgtacg gtcactcctt tggcgctcag
     1921 cttctgcgcc atctctttga cggcgtgctg cgcccgcgcg acagccaggc cgtaccctgg
     1981 cacgatgatg acgctctcgg cgttgcccag gaggaacgcc acgtcctcgg gcgagcccga
     2041 cctgaacgac ttctgctcgg tggccgcggc cgcgccctcg gcggccccga agcccccgag
     2101 gatgacgctg aagaacgacc ggttcatcgc cttgcacatg atgtacgaga ggatggcgcc
     2161 cgaggatccg acgagcgacc cggcgacgat gagcagcggg ttgttcagcg agaagccgat
     2221 gccggacgct gcccagcccg agtagctgtt gagcatcgag atcacgaccg gcatgtcggc
     2281 gccgccgatc gggatgatca cgagcacgcc cagcacgaac gcgacggccg tcatcgcgaa
     2341 gaaggacagc cactgggcgc cgggcggcgc gagcacgaac gacaccccga gcccgatcat
     2401 ggcgatcgcg agcgtcaggt tcaccacctg ctgtcctcgg aagacgaccg gggcgctcga
     2461 gaacagcctc gtcagcctgc cgaggccggc cagcttgccg aacgcgatca cggaccccga
     2521 gaacgtcatc gcgccgacga aggtcccgat gaacagctcc acgcggttgc cgacaggaac
     2581 gggatcgccc acgccgaacg ccgtggggtt gttcacgacg gcgatcgcaa tgagcacggc
     2641 cgccagcccg acgagcgagt gcatcgcggc gaccagctcg ggcatctgcg tcatctgaac
     2701 ccgtcgtgcc acgagccagc cgatggcgcc gccggccccg agcgcgagga gcgtcaggtc
     2761 cacgcgcctc gtaatcgcga gcgtcgtgag cacggccagg accatccccg cgatcccgaa
     2821 tcgattgcct cggcgcgcgg aaagggggtt cgacaatcct ttcagggcga ggatgaagag
     2881 cacgctcgcc gcgaggtacg agagggcgac caggttgggc gacatgctca cggcttcgcg
     2941 cgcggctcct tcttcttgaa catcgcgagc atgcgctggg tgaccaggaa cccgccgaat
     3001 gtgttgaccg ccgccagaac cacggcgatg aagccgagca tcgtgcccga gtccatccgg
     3061 cccgatccgg cggccagaat cgccccgacg acgatgaccg agctgatggc gttggtcacg
     3121 ctcatgagcg gcgtgtgcag ggcaggcgtc acgttccaca cgacgtggta gccgacgaag
     3181 acggccagca cgaacaccat gaggttcatg acgagcggat cgacggtggc tgtgggcacg
     3241 tcagctcctg accgcggcgc cgtcgatgca cacgagcgtg gccgcgatga tctcgtccga
     3301 ccggtccacc ctcagctccc ccgtcttcgc gtcgagcagg agcgtggtga agttgagcag
     3361 gttgcgcgcg tacagcgcgc tcgcatccgc cgccagcgag gcggggaggt tggagggcgc
     3421 gacgatcttc acgccgttga ggacgacgac cgtgtccttc acgctctgcg tgcaattgcc
     3481 gccctggctg acggcgaggt ccacgacgac cgagccgggc ttcatgccgg caatcgtgtc
     3541 ctccggcagc agaaccggcg ctggccgccc gggcaccaga gccgtggtga tgacgatgtc
     3601 ggcggccttc gcccgctcgg cgatgagcgc ggattggcgg cgcttgtaat catcgctcat
     3661 ctcgcgcgcg taccctccgg cggtgtcctg cgcccgcttc tcctcctcag acaggggtac
     3721 ctccacgaac ctggcgccga gcgactcgac ctgttctttc acggcggggc gtacgtcgaa
     3781 cgcctcgatc actgcgccaa gcctcttcgc ggtcgcgatc gcctggagcc ccgcaacccc
     3841 ggcgccgaga acgagcaccc gggccgcctt gaccgtgccg gcggcggtca tcaacatcgg
     3901 catgaaccgc ccatattcgt gggcggccac cagcaccgcc cggtaccccg cgatattcgc
     3961 ctgtgacgag aggacgtcca tgttctgggc acgcgtgatg cggggcagca gttccatggc
     4021 gaacgcggtc acacccgtgt gcgcgagcgc ggtcatgtcc tggtgcggcg tcagcagccc
     4081 cacgaggatg ctgccgcggc ggagcagcgg gagctccgtg tccgtcggtc gatgcacttt
     4141 caggacgatg tcggagctgg cgtagaggtc ggccgcgcag ccgagcgagg cgcctgccgc
     4201 tgcgaactcg cggtccggga tgctcgctcc tgcgccggcg ctggtttcga cggtgacggt
     4261 atgccgcccc ccggcggtga gcttcttcac cgtctcgggc gtcgcggcga cgcgtgtctc
     4321 tcctgggtag acctcggctg gaatgccgat gcgcatcagt tgaatccctc ggccgcaccc
     4381 ggattcaact gcccggaccg gctgcgtgtt ggcagcgtca tcttacactg cggctcgcgt
     4441 gtgttggggg caccgggctc caccaaacat attatcttca gccatggatc ttggactcac
     4501 cgatcgcgtc gcgatcatca ccggttcgag caggggactg ggcctcgcga gcgcgaaggc
     4561 cctgatctcg gaagggtgct gcgtgaccat ctgtggcagg acgcaggcca ctctggacga
     4621 cgccaggcgg cagctgacgg aactctcggg aagagacgaa cgggtgctga cggttgcggc
     4681 agacgtgagc cggccagacg gcgcgcacgc cgtcgtcgac aggaccatcc aggcgttcgg
     4741 cgccgtcgat gtactcgtga acaacgtggg gctggcgcgg ggctcgagcc tgctcgagac
     4801 gtctgacggg cagtggcagg aggcgatcga ccagacgctc ttcccggcga tccgtgtgtc
     4861 gcgcctcgct gtcccgcaca tgcagcgccg tgggggaggc tcgatcatca tgatcgcgtc
     4921 gatctacggc cgcgaggccg gtggccggat gacctacaac gccgtgaagg ctgccgagat
     4981 cagcctggcc aagtccctgg cgcagcaact cgccccgatg aacatccgcg tcaacagcgt
     5041 cgcgccgggc tccatcctgt tcgagggcgg atcgtggtgg aaacggcagc gcgacgaccc
     5101 cgcggcgatg gcggactacg tgaagcgcga gctcccgttt ggccgcttcg gccgcgccga
     5161 ggaagtgggc gacgtcgtcg cattcctggc gtcgcgccgc tcgagctgga tcagcggcgc
     5221 gtccgtggtc gtagacgggt gccagagccg ggcgttctaa aagcgaagcg cgccgcgtga
     5281 ccactgagac ccgttcagac gcctccaccg ttcgcgcggc ccgtcccgcg tttctcaccg
     5341 acatcggaat cgccgtgttc ggtgttctgc tgtttcagct ggtcatcgtt tcgtcgagcc
     5401 gattgacggc gaacggcggc ttcggctggg acggagagtc gtatgccggc atggtggcca
     5461 actcgctggc cagcggaaac ccgaataccc agatgcggcc gctgctcgtc ctgctcacgc
     5521 gtgttcccta cgcgctgggg ctcgacctgt accacagctt cgtcgcgctc gactacgtct
     5581 acgccttcgg cttgtacctg gcggcctccc tgctgctggc tcacgcaggc ctgacgtggc
     5641 cggtccgggc cctgctggtc gtcaacctgg ccctcagcat cggcacgtcg aagatgtatg
     5701 cgttctatcc gacgttgatc gactttggag cgctcgcgct ggtgacgtgg tccttctacc
     5761 tgtcaacgac cgatcgacat cgcctggctg gcctcgtgtg cattctggct gccgcagcgc
     5821 gggaattcgg cgtccctgcg gccttgtacg ggatgcatcg cgcgttccgg cagcgcgggt
     5881 gggcgaacgt cgcgatgtac gtacccagcc tcgtcacctt tgccctgatt cggtggcgcg
     5941 tgtcggcagc gttcgcgggt ggtccggacc agggccctct ggacctccag gatgcgatcg
     6001 acagactggc gctctgggcc tcaccctggt tccccgtcat gtttgcctat ttcacgacgt
     6061 cggtgtttgg cggcatcagc gcgctgctcg tgctccggct tcgatggtgc atcggccgcc
     6121 tccgagccga accggaactg gcgacgctgc tcgccctggt gggggcgggc gccgtggcgg
     6181 cgggcgggga cgtctggcgc tacctcgtgt ttgcgatgcc agccgcggtg gcgctcgcgg
     6241 ggaggtatct tgccggatgc gagccggatc tcaggcgccg cggcctcatc gcggtgacgg
     6301 ccgtcacggt gatcacccag cgcccgttcg agctgatgga ccgggcgaag tacttcctcg
     6361 actggtttcc cctctactac gtcctggacc tgctcgaacc gatcgacacc ttcgttcccg
     6421 tgtgggctgg ccgcctgctg gtcatggctc ttctcctcgt ggcgctttac ttcgcggcgg
     6481 tgtggaccgg acgccgttcg cacgtgcccg cagccggcaa cggctcgccg tcgtcacgcg
     6541 gctggacgct cgacgttctg gcggtgctgg cgctggccgc ggttgcctca tggccggtcc
     6601 agcgcgagct tcaggccccc gagccgccga cactcgaagc aggagcgcgc gacaaggtcg
     6661 gctgggagac ggaaaaccgc cgggtgcttc agggagacgt cccgctgatt gagtccggcc
     6721 ggctggcgcc acaatcgcag ctggcgcgcc cacaaacgct gttgctctat cctccgcacc
     6781 tcgttcttcg gttcgtgccg atcgcgctct tctttcccgt cagcctcgca ctccatgcct
     6841 ggctggctgg acttggtggc tacatggtgg cgaggcagtt aagggtgccc cgccttgcct
     6901 cgctcgcggc aggcgtcgcc gtgatgggtg ggggcgtcct catgcccgtg gccgatccct
     6961 tctcggacgg cgtgcagcaa ttggcgtgga tgcctctggc gttggcgctg acgatgcgat
     7021 cgaccgcccg cggtggcgtg tggcctcatc cggcactggt ggccatcgtg gcgatcggcc
     7081 tgctgtcggg atcgctgcgc ggcgcgatgt atgtcatcgg aaccgcaggc gcatggtatc
     7141 tgttcgacgt cctcgtgcca gtcgattctc gacctcggca caccgcgccc gcccggcagt
     7201 tcgcgatgct gtgtgcgctc ggtgcggggc tctgtgccgt tgtcctgctc cccgccttcg
     7261 agtcagcgct gtcgtcggac ggagcaggcg gtgtggtcag cctcgtgatc ggagtcggcg
     7321 tctcggcctc gaccgcctct gcctggttca gcacggaatt cgcggactac gggaaattca
     7381 tgcggcgtac gaggtccagg taacgcgcat ccggccgcag gcggtcgtat cgcgggttct
     7441 cgcggatgtg aatgagccac ccgttacgct gttcgaaggc cttgtccagc caggcgaacg
     7501 cctggtccag atcgccgagg ccgatgtgaa cctcggcgac ggctcgcgcc gcgacatagc
     7561 gttgggacgc cacccgtgtc atctcgtcga gaaccgcctg agcgtctgcg cgcctgcctg
     7621 cagcagcgta ggcgtgaccc aggtcggcaa ggtaggccgg atgtcgctgg gacagctcgg
     7681 cggccacctc gaactcctgt atcgcgctcg agcgttctcc aagctgctcg tatgcctggc
     7741 cgaggaacac gtgagcgagg atgaaggtgt ggtcgagttc gagtgttttc tggcactgct
     7801 cgacggcctt ctggtactgg cgcgaataca ggtgatgcca accaaggtgc atgttcagta
     7861 cgagcccgag cgggtcgcac tcgagcgccc gccggctctc tgcaagcgac tcctcggtcc
     7921 gattcagcgc cctcagcaaa tgcgagtacc agatgtgcac cgggccgtac tgcggcttga
     7981 gggcgatggc gtgtcgaagt cctcgctcgg ccgctgccca gtcccaatcg aagccaccgg
     8041 ccgtccaacc gagcgcggcg tgcgcttcgg cgcaattggg gtcgagttcc agcgcgcgca
     8101 gggcggcctg ctttgccttc gggtaggctt cagacggcag ggccaggccg aacagatccc
     8161 accccagcga gacatacgcg tcggctagcc cggcgtgggc gagcgcgtag gtcgggtcga
     8221 ggtcgatggc ctgctggagg ctctcgactc ctcgcttcaa cgcgtcgggg ctccgcttgt
     8281 accagagatg acggcccttc aggaacgctt cgtacgcgac cggatcgacc gggcggcttc
     8341 cactcagccg cgttttttcc gccggtgaca actggactcg aatcccgtcc gcgatggcgc
     8401 gggcaacctc gctctgcagg gcgagcacgt cgcgcaggtc ggtttcgtac tgctttgccc
     8461 agaggtgcgt atcggtggcg gcgtgaatca gctgggcggc gattcgaatg cggccgtgcg
     8521 accgcaacac cgtcccttcg acgacggcgt cgacgttgag ctcgcgcgcg atctcgggca
     8581 gccccagtcg agtgcccttg tagcgcatca ccgacgtgcg cgaaatcacg cgcagcgcgc
     8641 cgatctgggc cagcgtggtg atgagcgcgt cggtcatccc gtcggcgaaa aacgcctcgc
     8701 ccgattcggc cgagaggtct tcgagcggca gaacggcgag cgatcggatc cgggctcgag
     8761 gcgatggacg cgaccccgat cggccacccg gcgaacggcg aggcgactcg agggcgtcga
     8821 gcgctgcgtg aacctggccg gcgtcctgat atcgctcgcc cggatccttg gcgaggcagc
     8881 gcaggacgat tttcttcagg ccgggcgcca cctgttcccc cagtgggagc ggggcggctc
     8941 caagaatgga ggagctcaac gtaaacggcg tgtcaccctc gtgggggcgc cgtccggagg
     9001 ccagttcgta gagcacgacg ccgagcgacc acacgtcgct tcgggccgtc gctggctcgc
     9061 cccgcagctg ttccggcgcc atatacggca gcgttccggc cagatcgtcg ctgaacgacg
     9121 agggcgtctg cgtcgtgcgc tcgaccacct gtgcgcccac gtgtcttgcc agcccgaagt
     9181 ccaggacctt cgttcgcccc tggcgcgtaa ccaggatgtt cgcgggcttg acatcgcgat
     9241 ggacgacgct cctggcgtgc gcgtgggcca gcgcatcggc gacctggaga ccgtaggcgc
     9301 gcgccgattc gaacggaagg ccgccggctg ggatcaggtc gctgagacgc tgtccatcga
     9361 ccagctccat cacgatgaac gcctgctggc cgtcgtcctc gacttcgtag accgtgcaga
     9421 ttcccggatg attcagcgcc gacgatgccc gcgcctcgtg cagcaactga cgagtaccgg
     9481 caccgcttga ttcgtcgtgg agcagcttga tggcgacgtc ccgccggagc ttttcgtcgt
     9541 atgcgcgata gaccgttccc attccgcccg taccgagtct ggcgtccaca cgataccggc
     9601 cgatttgatg aggagcgtgc gccttcatgg gctgaggtat cgaaacgacg aatgccgatt
     9661 gtattacgct ctcatcgacc tggagagacc atcatgtcgc gccgactcct cgacgctcag
     9721 ctgtccccgt atctcgacgg cctcgtgccg ccgaggccgc ctgagatggc cgccatggag
     9781 gcggaagccg cgaagaccga ctttccgatc atcggtccga cggccggcca cgtgtgttac
     9841 ctcatcgcgc ggctgatcgg agcccgatcc gtgttcgagc tcggttccgg cttcggctac
     9901 tcgacagcct ggttcgcgcg cgcggtacga gacaatggcg gcggcgtcgt tcaccacacc
     9961 gtgtgggatg acgaactgtc gcgccgcgct cgaggccacc tgagcgcgct cggctacgcg
    10021 gacctcgtga cgtttcacgt tggggaatcg gttgcggcgc tgcgggagtc cacgggcccc
    10081 ttcgacttga tcttcaacga catcgacaag gcgggctatc cggcgtcgct tcccgtgatc
    10141 gcgtcccggc tgcggcccgg cggcgttctc atcgtggaca acatgctctg gagcgggcgc
    10201 atcttcgacg aagccgatcg atcggcccac acgcaaggcg ttcgcacgct gacgcagctc
    10261 gtcaccgccg atccggactg gaccgccagc atcattcccg tcagggatgg cctgctcgtg
    10321 gctcagcgga accctagagg ccgggccggg gcgccggttc ggcctgaacg gacttgacga
    10381 gcagcggccg ccccggagaa gtctcgatga cgaccgggtt gatggcgtcg atcctgattg
    10441 gaagcggcgc ttcgatcggg agtggccgat ccaccgtgac cctcacgggt gcggtggcct
    10501 ccgcaatccg cacggggaag accagcggcc tcccgcgcac tccctcgacg cccttgatga
    10561 tgacgggcat cggctcgacc tgctgggccg acacagggac ggccgcatcc ttgactccga
    10621 gccatcccaa ctcgacggcg atgaccgtca gaacgatctt cgtgtagcgg tcggctgaca
    10681 tcgtaatcct cctgaccacg tcttccgagt tttgagtttt gagttacagc gccttcctat
    10741 atcgccttga ccgacttcaa cagggctgtc gaggcctggg ccagcatgac cgtgtccacg
    10801 cccgcgacga tcatcgtgaa gccctgctcg atgtacggcc gcacggcgtc ggcggtgagt
    10861 ccgaagatcc ctattggaag gtgcgcctcg cgacaggcgt cactcacgcg cgcgatcgca
    10921 tccttcacgt cctggtgatc gactctgccg ggaatgccca ggcttgctga cagatcgtac
    10981 ggcccgatca ggacgccgtc cacgccctcg acgcggacga tctcccggat gtgcttcacc
    11041 gcctcgtggt gttcggcctg cacgatgacg gcggtgtcct cgttggccgt gtgcaggtac
    11101 tccggcacct tcaagccgta gcgatgggcc cgtgcgatgc ccatgcctcg tcgtccagcc
    11161 ggcgcgtatt tggaccactg gacgatctga cgcgcatgct cggccgagtt gatctgcggc
    11221 gcgatgatgc cggccgcgcc gatgtcgagc gcctgcttga cgtggatctc gtcgctcgat
    11281 gcgagccgga cgacgcaggg cgtggccccg gcagcctgca tcaaggcttg catctcgagg
    11341 gcgtcgaagg gcgcatgctc ggcgtcgaga aacagccaat cgaagccggc ttcggcaagc
    11401 agctcgacga ccggtggcga gttgagcgtc acgagcggcg cgatcacgcg ctcgcccgac
    11461 cggagtcgcg cgcgaaaggt aggtggtggc actaggctcg tcgtcgtccg acggcaaaca
    11521 gcgacagccc aatcggcggc ggcacgatcc gctcgagcat cagcgcccac ggcgccacca
    11581 gccggtcgaa cagcctgacc tgcgaagggc tgtgcgaagt cgccttgctg atatgcgcgt
    11641 tgt
//
LOCUS       DBNGSH010000213         3069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4261838, whole genome shotgun sequence.
ACCESSION   DBNGSH010000213 DBNGSH010000000
VERSION     DBNGSH010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4261838"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="AC16CY_04825"
     CDS             complement(<1..895)
                     /locus_tag="AC16CY_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3353136.1"
                     /translation="MASYSSYSYDVLIVGAGGAGLRAAIEAASGNVSVGLICKSLLGK
                     AHTVMAEGGMAAALANNDDRDNWRVHFADTMRGGQYVNNWRMAELHAKEAPDRVRELE
                     GWGAVFDRTGDGRINQRNFGGHRFPRLAHVGDRTGLELIRTLQDHAVYLGMTVHMEHT
                     VIDLILDNGRAAGVLAYDRERGRFHVFAAKAIVLATGGIGRAYKITSNSWEGTGDGHA
                     LAYRAGAELIDMEFVQFHPTGMVWPPSVRGILVTEGVRGEGGVLKNSEGRRFMFDDIP
                     DNYKPQTASDAEEGWRYTQGDK"
     gene            complement(895..1698)
                     /locus_tag="AC16CY_04830"
     CDS             complement(895..1698)
                     /locus_tag="AC16CY_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HIR3353137.1"
                     /translation="MEAIPVRIVPPGFGETRRRDMWWLEPMTVFIVFSGFLLYGTWAA
                     YQNAHYTYGPYLSPFYSPELWGASEHAWFGPKPAWWPGLIPFSPGWLILPFPGLFRLT
                     CYYYRGAYYKAFWMDPVSCTVGEPRSTYWGERSFPLILQNVHRYTAYIILMFIVLLSY
                     DVWLALWFTDPATGQKTFGVGLGTLVLAVNVVLLAGYVFGCHSIRHLVGGLKDEVSKS
                     PVRHACYNCSSALNGKHMLFAWTSLFSVMLADVYIRLCSMGVLTDVRIL"
     gene            complement(1764..2747)
                     /locus_tag="AC16CY_04835"
     CDS             complement(1764..2747)
                     /locus_tag="AC16CY_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353138.1"
                     /translation="MSRLLALGLAFGVLGAALAAQAPAEVEKHIAAAWAAAGTEHAGM
                     VTRLCPASAPAAAAAPAARGGGARRGGGPPSPPPRESWHAEPVKVFDNFYFVGMTEYS
                     TWAITTSQGIIVIDPVFDYSVEDEVVGGLTKLGFNPADIKYVLISHGHLDHAGGAKFL
                     QERFGARLLMAAADWDRLEKQNPPWKPRRDMVVTDGQALTLGDTTLTMYLTPGHTDGT
                     VSTIIPLRDGKERYVAALWGGTLFNFGPDRARFASYAASALRFRDVATKAGARVLLSN
                     HTEYDGSKTKLPAMATRKPGGPHPYVVGLDSVQRYLTVANECAQAALAGLP"
     gene            complement(2750..>3069)
                     /locus_tag="AC16CY_04840"
     CDS             complement(2750..>3069)
                     /locus_tag="AC16CY_04840"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010621.1"
                     /note="E3 component of pyruvate and 2-oxoglutarate
                     dehydrogenase complex; catalyzes the oxidation of
                     dihydrolipoamide to lipoamide; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3353139.1"
                     /translation="QASGIEFEVAKFPWGASGRAITVDRPEGLTKLVVEPKTGRILGV
                     GIAGSGAGELIAEGVLAIEMGATAEDLKLVIHPHPTLSETVMESAEVFFGQSTHVYRP
                     KRK"
BASE COUNT          526 a         1044 c         1002 g          497 t
ORIGIN      
        1 ccttgtcgcc ctgggtgtac cgccagcctt cctcggcgtc cgatgccgtc tgcggcttgt
       61 agttgtccgg gatgtcgtcg aacatgaaac ggcggccttc gctgttcttg agcacgccgc
      121 cctcgcctcg cacgccttcg gtcaccagga tgccgcgcac gctcggaggc cagaccatgc
      181 cggtcggatg gaactgcacg aactccatgt cgatcagctc ggcgccggct cgatacgcga
      241 gcgcgtggcc gtcgccggtc ccctcccagc tgttgctcgt gatcttgtag gcacggccga
      301 tgccccccgt cgccaggacg atcgccttgg cggcgaacac gtggaagcgg ccgcgctccc
      361 ggtcgtacgc caggacgccc gccgcacggc cgttgtcgag aatcagatcg atgaccgtgt
      421 gctccatgtg cacggtcatg cccaggtaga cggcgtgatc ctggagcgtc cgaatcagct
      481 ccagccccgt gcggtcgccg acgtgagcca ggcgcggaaa ccggtggccg ccgaagttgc
      541 gctggttgat gcgcccatcc cccgtccggt cgaataccgc gccccacccc tcgagctcgc
      601 gcacgcgatc gggcgcttcc ttggcgtgca gttcggccat ccgccagttg ttgacgtact
      661 ggccgccgcg catggtgtcg gcgaaatgga cgcgccagtt gtcgcgatcg tcgttgttgg
      721 cgagcgccgc cgccatgccg ccctcggcca tcacggtgtg cgccttgccg agaagcgact
      781 tgcagatcag gccgaccgat acgttgcccg aggccgcctc gatcgccgcg cgcagcccgg
      841 cgcccccggc gccgacgatc agcacgtcgt agctgtagct cgaataggat gccatcagag
      901 aatcctcacg tcggtcagga ctcccatcga gcagaggcgt atgtagacgt cggccagcat
      961 cacggagaag aggctggtcc acgcaaacag catgtgcttg ccgttgagcg cgctcgagca
     1021 gttgtagcag gcgtggcgga ccggcgattt cgagacctcg tccttcaggc cgccaacgag
     1081 gtggcggatc gagtggcagc cgaagacgta gccggcgagc agcacgacgt tcaccgcgag
     1141 cacgagggtg cccagcccga cgccgaacgt cttctgcccg gtcgccgggt cggtgaacca
     1201 gagcgcgagc cagacgtcgt aggacaacag cacgatgaac atcaggatga tgtacgcggt
     1261 gtagcggtgg acgttctgca ggatgagggg aaagctccgc tcgccccaat acgtgctgcg
     1321 cggctcaccc accgtgcacg acaccgggtc catccagaac gccttgtagt aggcaccgcg
     1381 atagtagtag caggtcaggc ggaagagacc cgggaacggc agaatcagcc acccgggaga
     1441 aaacggaatc agccccggcc accacgccgg cttcgggccg aaccaggcgt gctccgacgc
     1501 gccccagagc tcgggcgagt agaacgggga caggtacggg ccgtaggtgt agtgcgcgtt
     1561 ctggtacgcg gcccacgtgc catagagcag gaacccgctg aacacgatga acacggtcat
     1621 cggttcgagc caccacatgt ctcgacgccg ggtttcaccg aacccgggcg gaacaatcct
     1681 gacggggatt gcttccatcg actgcctccg aatgggacga aagacgagcg gcaaactttg
     1741 ttaccgtagt cggtgtacga cgatcatggc aagccggcca gcgccgcctg cgcgcattcg
     1801 ttggcgacgg tgaggtagcg ttgcacggag tcgagaccca cgacgtacgg gtgcggtccg
     1861 cccggcttgc gcgtggccat ggcaggcagc ttcgtcttgc tgccgtcgta ctcggtgtgg
     1921 ttcgagagca ggacgcgcgc gcccgccttc gtggcgacgt cgcggaagcg cagcgcggag
     1981 gccgcatacg aggcgaacct cgcgcgatcg ggtccgaagt tgaacagcgt gccgccccat
     2041 aacgcggcaa cgtagcgctc cttcccgtcg cgaagcggga tgatcgtgga cacggtgccg
     2101 tcggtgtgcc ccggcgtcag atacatcgtc agcgtcgtgt cacccagcgt cagcgcctgg
     2161 ccgtcggtca cgaccatgtc gcgcctgggc ttccagggcg gattctgctt ctcgagcctg
     2221 tcccagtcgg cggccgccat cagcagccgc gcgccgaacc gctcctgcag gaacttcgcg
     2281 ccgcccgcgt gatcgaggtg accgtgactg atgaggacgt acttgatgtc ggcgggattg
     2341 aagcccagtt tcgtaagccc tccgaccact tcgtcctcga ccgagtagtc gaacaccgga
     2401 tcgatgacga tgatgccctg cgaggtcgtg atggcccacg tggagtactc cgtcatgccg
     2461 acgaaataga agttgtcgaa gaccttgacc ggttcggcgt gccacgactc ccgaggggga
     2521 ggcgagggtg gtccgccgcc gcgccgggca ccgccgccgc gggcggccgg cgccgcagcc
     2581 gcggcgggtg cagaggcggg acacagcctc gtaaccatgc ccgcgtgctc ggtccctgcc
     2641 gcggcccagg ccgccgcgat gtgcttctcc acctccgcgg gcgcctgcgc cgcgagagcc
     2701 gcacccagaa ccccgaacgc caaaccgaga gccagaagac gactcatctc tacttcctct
     2761 taggcctgta gacgtgcgtg ctttgcccga agaacacctc ggcgctctcc atgaccgtct
     2821 ccgacagcgt cgggtgcgga tggatgacga gcttgaggtc ttctgccgta gcgcccatct
     2881 cgatggccag cacgccctcg gcgatgagct cccccgcccc ggatccggca atgcccacgc
     2941 cgagaatgcg cccggtcttc ggctcgacca cgagcttggt caacccctcc ggccgatcga
     3001 ccgtaatggc gcggcccgac gccccccacg ggaacttggc cacttcgaac tcgattcctg
     3061 atgcctgcg
//
LOCUS       DBNGSH010000214         8054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_526556, whole genome shotgun sequence.
ACCESSION   DBNGSH010000214 DBNGSH010000000
VERSION     DBNGSH010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_526556"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC16CY_04845"
     CDS             <1..270
                     /locus_tag="AC16CY_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3353140.1"
                     /translation="VAPDDLESIPDRIDGEQQLDRRRVLDKLYELIRQLPPIDRQVML
                     LYLEEVDAAAIADITGLTAGSVATRIHRIKQTLARRIHQRDTEHE"
     gene            263..811
                     /locus_tag="AC16CY_04850"
     CDS             263..811
                     /locus_tag="AC16CY_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353141.1"
                     /translation="MNEPERDIQRLWRGQPREEQVMSIDEIRSRAERFEQRVRRWGVV
                     GGSLFALILVVESWQVWRSPELPERVGDLLTMAALVFAAYLYRPYMTTQAMPSSLGLT
                     SSVEFYRTQLARRRDLASHPWRYLVLFVPGVGLSLFGDALTRPVFQTAAIAAFGVALF
                     LWAAWVNGRTARRLQREIDELG"
     gene            926..2539
                     /locus_tag="AC16CY_04855"
     CDS             926..2539
                     /locus_tag="AC16CY_04855"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HIR3353142.1"
                     /translation="MTTEKHPEDARLLSLDEPPAVELPAGLDRRAFMMRSALVGATAV
                     ITGCSAPSEQQVATASPPPPAPAAPPLSEDLNVVKKAKGPVMTTLDEFYKVGPGPSSS
                     HTIGPMRITYDFYQRCTKLPADQLAKATGLKVHLFGSLSATGKGHGTERAALAGIIGK
                     EPATVEPEFLDSLSAKPDQTFQVKLGDKTHTVGIKDIVYDAPKGDFPHPNTMTCRLMA
                     GDQVLHELEYYSVGGGFIEWKGYEPPKKGQPKFPYATMKELQAHAEKSKLSIAQVVMA
                     NEVAVSGKSEAEIHAFIDKISGAMVNIVKAGLKAPPATLPGPIKLKTKAGDVYTRAMD
                     DKYERQRGVGVVAAYALAGSEENARGHLVVTAPTGGSAGVMPALVYALGEGGRNLPQS
                     KIRDGFLAAAAVGYLCKHNATLSGAEGGCQAEIGVASAMGAAFIAQAHDFGHQVVANA
                     AESSLQHHLGMTCDPVAGFVQVPCIERCAFGAVKAWTGFMIASNEIPTNRRVDFDTTV
                     SAMALTAKEMNAKYKETSEGGLAVSLVLC"
     gene            2567..3832
                     /locus_tag="AC16CY_04860"
     CDS             2567..3832
                     /locus_tag="AC16CY_04860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3353143.1"
                     /translation="MNRGLQTAAIAWLVTAVYYFYQYTLRSAPAVMMPQLSDAFGLSA
                     LGVASMVGLFYYGYSPFSLVAGVAMDQLGPRKVIPVGAALVGIGALLFGTGNSEAASI
                     GRLVQGAGGVFALVGAAYIATTNFPASRAATLIGATQMFGMAGGSAGQVLVGPAIASG
                     VSWSTFWIAMGLVGLAISVILARLLPEPETKARRADWLTSAAGAMKTVFLNPQSILCG
                     MIAGLLFIPTTIFDMVWGVRYLEEARGLDYGSAVMRSSMVPLGWIIGCPLLGALSDRI
                     GRRKPVIVAGAVVLFACLAWILYGRADVFPPYSVGLLAGLASGAAMLPYTVIKEANPP
                     GMSGTATGVVNFLNFTFSALLGPVFGWLLVTVSGGASPMTLDHYQMAFQPLLYGVALA
                     MVLTLLLKETGSAARAVSAPLPLPHREAI"
     gene            3829..4773
                     /locus_tag="AC16CY_04865"
     CDS             3829..4773
                     /locus_tag="AC16CY_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HIR3353144.1"
                     /translation="MTRETGTGKYEQALSRCRSLEPVSTAVAHPCDEASLAGAFEARA
                     NGLIIPILVGPAARIREVAARHGIDLGNTRIVDAAHSHESAAKAVALVRQGDAELLMK
                     GSLHSDELLGAVVARETGLRTARRISHVFIMDVPTYHKVLLVTDAAINIAPTLEEKVD
                     ICQNAIDLMIALGKPTPKVAILAAVETVNSKMQATIDAACLCKMSERGQITGGILDGP
                     LAFDNAISKEAARMKGIASEVAGDPDILLAPDLEAGNILAKQLTFLANADSAGIVLGA
                     RVPIILTSRADSVRSRSASCAVAVLAAHARRKALQATV"
     gene            4791..5987
                     /locus_tag="AC16CY_04870"
     CDS             4791..5987
                     /locus_tag="AC16CY_04870"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR3353145.1"
                     /translation="MDEYAVIINAGSSSLKFSVFRRRDVDAWRLEVRGQVEGIGTRPQ
                     FSAKDEAGQPVGDHQVDAAVKDGRAALAHVADWLRERYGGARVRGIGHRVVHGGARYT
                     GPTIVTPEVLDDLRTLIPLAPLHQPHNLAAIEAVWERLPGVPQVACFDTSFHRGQPAV
                     AELVPLPSEIRDAGVQRYGFHGLSYEYIASVLPSVAPAIAHGRVIVAHLGSGASMCAL
                     RNGRSVDSTLGFTALDGLCMGTRPGAVDPGVVLHLFQSLGLSAQEVESILYKKSGLLG
                     ISGISNDMRALLASTEPAARLAVDYFVYRAAKEIGALAAVLGGIDGLVFTAGIGENSA
                     EVRKRICQASAWLGLELDDGANARTESRISTPASAVSAWVIPTNEELMIARHTGLLLG
                     LVEARV"
     gene            6039..>8054
                     /locus_tag="AC16CY_04875"
     CDS             6039..>8054
                     /locus_tag="AC16CY_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HIR3353146.1"
                     /translation="MRLSPVLLLLAVAPLAAQQPLQRTAEDYARAERFLAQNTSPLVG
                     GVAGQPRWLADGRFWYRVTRPGGGAEFVMVDPSRRTRAAAFDHTRLAAGLGEATKTKV
                     EATRLPFQSFEFSKDGREVTVTALGRSWRCRIESYTCRAPEPAQPAGGSSAASSNSVT
                     SPDGRYAAYIRDFNLWVRDLAANQDRQLTTDGVKDFGYATNNAGWIKSDAPVLLWSPD
                     SKKIATFQHDDRGVSEMYLVSTAVGAPRLEAWKYPLPQDKEIFRIHRVVVEVDPARVV
                     RFQMPPDQHRSTISDHVAEGSTFLDVQWYPDGTHVAFVSSSRDHKEAIFRVADASTGQ
                     VRTVFEEKSRTQFQSGLAAVGEPNWRVRPSANEVLWWSQRDDWGHLYLYDLESGRLKR
                     QVTSGNWNVAQLVHTDERSRTLYFTAVGRERGRDPYFQHLYRAGLDGQGVTLLTPEPA
                     NHTITMAPDGQTFLDSYSTPTTPPVSVLRRMDGEVSLELERADISRLVAAGWKPPTPI
                     VVKARDGRTDLYGLMFTPTGLDESRKYPIVNYIYPGPWGSSVGGRSFSAARADHQALA
                     ELGFVVVVIDGMGTEWRSKSFADFYYGDIGDNTLPDQIAGMRELAKRHAYIDIDRAGI
                     WGHSGGGFAAAAAMFRHPEFFKVGVSQAGNHDNRNYEDDWGERFQGLL"
BASE COUNT         1329 a         2654 c         2780 g         1291 t
ORIGIN      
        1 gtcgcgccgg atgatctcga gtcgatcccg gaccggatcg acggagagca gcagctcgat
       61 cgccggcggg tgttggacaa gctctacgag ctgatacggc agctcccccc gatcgatcgg
      121 caggtcatgc tcctctatct cgaggaggtt gacgccgcgg cgatcgcgga catcaccggg
      181 ctgaccgcgg gaagcgtggc gacgaggatt caccgcatca agcagacgtt ggcacggcgc
      241 atacatcagc gagacaccga acatgaatga gcccgaacgg gacatccaga ggctgtggcg
      301 cggccagccg cgagaggagc aggtcatgtc gatcgacgag attcgttcga gggcggaacg
      361 cttcgagcag cgcgtacggc gctggggtgt ggttggagga tccttgttcg cgctgattct
      421 cgtcgtcgaa agctggcaag tgtggcggag tcccgagctg cccgagcggg tgggcgacct
      481 gctgacgatg gccgcgctcg tctttgcagc gtacttgtat cgtccgtaca tgacgacgca
      541 ggcaatgcca tcgagcctgg ggttgacgag ctcagtcgag ttctaccgga cgcagctcgc
      601 gcgccggcgc gatcttgcga gccacccgtg gcggtacctc gtcctgttcg tgcccggggt
      661 ggggctgagc ctgttcggcg acgcgctgac ccggccggtc tttcagaccg ccgcgatcgc
      721 ggctttcggc gttgcgctgt tcctgtgggc cgcgtgggtc aacggccgca cggcccggag
      781 gcttcagcgg gagatcgacg agctcggctg aggacggctt gcggccacgc gccactgaaa
      841 cagctgcgct gcgtctcgct gctacaatgg ccccggcgtg cctgcgcctg accgctggaa
      901 cgtggatgtc caggagggaa cacacatgac aaccgagaag catcctgaag atgcgcgcct
      961 cctgtccctc gacgagcctc ctgccgtcga gctaccggcg ggcctcgatc ggcgcgcgtt
     1021 catgatgcgc agcgccctcg ttggcgcaac cgcggtgatc acagggtgct ccgcgccgag
     1081 cgaacagcag gtcgccacgg cgtcgcctcc tccaccggcc cccgccgcgc cgcctctctc
     1141 cgaggacctg aacgtcgtca agaaggcgaa gggtccggtg atgacaacgc tggacgagtt
     1201 ctacaaggtc gggccggggc cttcgagttc gcacaccatc ggcccgatgc gcatcaccta
     1261 cgacttctat cagcgatgca cgaagcttcc cgcggaccag ctcgccaagg ccacggggtt
     1321 gaaggtgcac ctgttcggca gcctgagcgc gaccggtaag gggcacggca ccgagcgcgc
     1381 cgcgctcgcc ggcatcatcg gaaaggaacc ggcgacggtt gaaccggaat tcctggacag
     1441 cctgagtgcg aagccggacc agacgttcca ggtcaaactg ggtgacaaga cgcacaccgt
     1501 cgggatcaag gacatcgtct acgacgcacc caagggcgat tttcctcacc cgaacacgat
     1561 gacctgcagg ctcatggccg gcgaccaggt gctccacgag ctggagtact actcggttgg
     1621 cggcggcttc atcgaatgga agggctacga gccgccgaag aagggccagc cgaagttccc
     1681 gtacgcgacc atgaaggagc tgcaggcgca cgcggagaag agcaagctct cgatcgcgca
     1741 ggtcgtgatg gccaatgaag tggcggtctc cggcaagagc gaggcggaga tccacgcgtt
     1801 catcgacaag atttccggcg cgatggtcaa catcgtgaaa gccggactga aggcgccgcc
     1861 ggccacgctg ccggggccta tcaagctcaa gaccaaggcg ggagacgtgt acacgagggc
     1921 catggacgac aagtacgagc ggcagcgcgg cgtgggtgtg gtcgccgcct acgctctggc
     1981 cgggtcggag gagaacgccc gcggccatct cgtcgtgacc gcgccaaccg gtggatcggc
     2041 gggcgtgatg ccggcgctcg tgtacgcgct cggagaaggc ggccgcaacc tgccacagag
     2101 caagatccgc gacgggttcc tcgcggccgc cgcggtcggc tacctgtgca agcacaacgc
     2161 gacgctgtcg ggcgcggaag gcggctgtca ggcggaaatc ggcgtcgcat cggcgatggg
     2221 cgcggcgttc atcgcgcagg cgcacgactt cggccatcag gtcgtggcca acgccgcgga
     2281 gtcgtcgctg cagcatcacc tgggcatgac ctgcgatccg gtggccggct tcgtgcaggt
     2341 gccgtgcatc gagcggtgcg cgttcggcgc cgtgaaggcc tggaccggat tcatgatcgc
     2401 cagcaacgag atcccgacca accgccgcgt cgacttcgac acgaccgtct cggcgatggc
     2461 gctcacggcg aaggagatga acgcgaaata caaggagacc tcggaaggag gcctcgccgt
     2521 gtcgctcgtg ctctgctgac aggctccaca cggagttcga acgacgatga accgcggtct
     2581 gcagacggcg gcaatcgcgt ggctcgtcac ggcggtgtac tacttctatc agtacacgct
     2641 ccgctcggcg cccgcggtca tgatgccgca actctcggac gcgttcggcc tgagcgcgct
     2701 gggcgtcgcg tcgatggtcg gcctcttcta ctacggctat tcgccgttca gcctggtcgc
     2761 cggcgtggcg atggatcaac tggggccgcg caaggtcatt ccggtcggcg cggccctggt
     2821 ggggatcggc gcgctgctgt ttggcacggg caatagcgag gccgccagca tcggccggct
     2881 ggtgcaaggc gccggcggcg tgttcgccct cgtcggcgcc gcctacatcg cgaccacgaa
     2941 ctttccggcg tcccgcgcgg ccacgctcat cggcgccacg cagatgttcg gcatggcggg
     3001 cggctccgcg ggccaggtcc tggttggacc ggcgatcgca tccggcgtgt cgtggagcac
     3061 gttctggatc gcgatgggcc tcgtcggcct ggcgatcagc gtgatccttg cccggcttct
     3121 gcccgagccg gagacgaagg cgcgccgcgc cgactggctg acgagcgccg cgggggccat
     3181 gaagacggtc ttcctcaatc cgcagtcgat cctgtgcggg atgatcgcgg gcctgctgtt
     3241 catcccgacg acgatcttcg acatggtctg gggcgtgcgc tacctcgagg aggcacgcgg
     3301 cctcgactac gggtccgccg tgatgcggtc gtcgatggtg ccgctcggct ggatcatcgg
     3361 ttgtcccctg ctcggtgcgc tctcggatcg catcggccgg cgcaagccgg tcatcgttgc
     3421 cggcgccgtg gtgctgtttg cgtgcctcgc gtggattctc tacggccggg cggacgtgtt
     3481 tcctccctac agtgtgggtc ttctcgccgg actcgcgtcg ggtgccgcca tgctgccgta
     3541 caccgtcatc aaggaggcga acccgccggg catgagcggc acggcgacgg gcgtcgtcaa
     3601 cttcctgaac ttcacgttca gcgcgctcct cggtcccgtc ttcggctggc tgctcgtcac
     3661 ggtgagcggc ggtgcgtcac caatgacgct cgaccactac cagatggcgt ttcagccgct
     3721 gttgtacggc gtcgcgctgg ccatggtatt gacgctgctc ctcaaagaaa cgggatccgc
     3781 ggcacgagct gtatcggcgc cgcttcccct tccacatcgc gaggccatat gacgcgagaa
     3841 acgggaaccg gaaagtacga gcaggcgctg tcacggtgcc gcagcctgga accagtgtcg
     3901 accgccgtcg cgcacccgtg tgacgaggcg tcgctggccg gggcattcga agccagggcc
     3961 aacggcttga tcatcccgat cctggtcggc ccggccgcca ggattcgcga ggtggccgcc
     4021 cgccatggaa tcgatctcgg caacacccgc atcgtcgatg ccgcacacag ccacgagtcc
     4081 gccgcgaagg cggtggcgct ggtccggcaa ggcgacgcag agctcctgat gaaaggcagc
     4141 ctgcatagcg acgagctgct gggcgcggtc gtggcgcgcg agaccgggct gcgcaccgca
     4201 cgccgcatca gccatgtctt catcatggac gtgccgacct atcacaaggt cctgctcgtc
     4261 accgacgcgg cgatcaacat cgccccaacg ctcgaggaga aggtcgacat ctgccagaac
     4321 gcgatcgatc tgatgattgc gctgggcaag cccacgccga aggtcgccat ccttgctgcc
     4381 gtcgagaccg tgaattcgaa gatgcaggcg acgatcgacg cggcgtgcct gtgcaaaatg
     4441 tccgagcgcg ggcagatcac cggcgggata ctcgacggcc cgctggcctt cgacaacgcg
     4501 atcagcaagg aggcggcgcg gatgaagggg atcgcgtcgg aggtcgccgg cgatcccgac
     4561 atcctgctcg cgcccgacct cgaggccggg aacatcctgg ccaaacagct cacgttcctc
     4621 gccaatgccg acagcgccgg catcgtgctc ggcgcgcgtg tgcccatcat tctgaccagc
     4681 cgcgccgaca gcgttcgatc gcgcagcgcc agctgcgcgg tcgccgtgct cgcggcgcac
     4741 gcgcggcgca aggcgttgca ggcgaccgta tagccgccgg gagccctggc atggacgagt
     4801 acgccgtcat catcaacgcc ggctcctcga gcctgaagtt ctccgtgttc cgccgccggg
     4861 atgtggacgc gtggcggctc gaggtgcgcg gacaggtcga aggcatcggt acccgcccgc
     4921 aattctcggc caaggacgag gcgggacagc cggtcggcga tcatcaggtc gatgccgccg
     4981 tgaaggacgg ccgcgcggcg ctcgcgcacg tcgccgactg gctgcgcgag cgctacggcg
     5041 gcgcccgcgt gcgcgggatc ggccaccgcg tcgtgcacgg gggcgcgcga tacacgggtc
     5101 cgacgatcgt cacgccggag gtcctcgacg atctgcgcac gctgatcccg ctcgcgccgc
     5161 tgcaccagcc gcacaatctc gcggcaatcg aggcggtctg ggaacggctg ccgggcgtgc
     5221 cgcaggtggc gtgcttcgac acgagcttcc atcgcggcca accggccgtc gccgagctcg
     5281 tgcccctgcc atccgagatt cgtgatgccg gcgtgcagcg ctacggcttc cacgggctgt
     5341 cgtacgaata catcgcgtcg gtgctgccgt cggtcgcgcc tgcgatcgcg cacgggcgcg
     5401 tcatcgtcgc tcacctcggc agcggcgcga gcatgtgcgc cctgaggaac gggaggagcg
     5461 tcgacagcac gctcggcttc acggcgctcg acggtctctg tatgggcacg cggccgggtg
     5521 cggtcgatcc cggcgtcgtc ctccatctgt ttcaaagcct cggcctctcg gcccaagagg
     5581 tggagtcgat cctctacaag aagtccgggc ttcttggcat ctcgggaatc agcaacgaca
     5641 tgcgcgcgct gctcgcgagc accgagcccg cggcccgtct tgccgtggac tacttcgtgt
     5701 atcgcgccgc gaaggagatc ggggcgctcg cagcggtgct cggcggcatc gacggcctcg
     5761 tgttcacggc ggggatcggc gagaactccg ccgaggttcg gaagcggatc tgtcaggcgt
     5821 cggcgtggct cggcctcgag ctggatgacg gcgcaaacgc gcggacagag tcgcgtatct
     5881 ccacccccgc cagcgcggta tcggcatggg tcattccgac caacgaggaa ctgatgatcg
     5941 ccaggcacac ggggctgctg ctcggcctcg tcgaggcacg cgtgtagacg tcaggcctga
     6001 cgtcgcttcc acagtggcgt gcgagttatg ctgacgccat gcgactgagt cctgttcttc
     6061 tgctcctcgc cgtcgccccg ctcgccgcgc agcagcctct gcagcggacg gccgaggatt
     6121 acgcgcgcgc cgaacggttt ctcgcgcaga acacgtcgcc gctcgtgggg ggtgtcgcgg
     6181 gccagccccg ctggcttgcg gatggccgct tctggtaccg ggtgacgcgg ccggggggcg
     6241 gagcggagtt cgtgatggtc gatccgtcgc gacggacccg cgcggccgcc ttcgatcaca
     6301 cgcggctggc cgccgggctt ggcgaggcaa cgaagacgaa ggtcgaggcc acgcggctgc
     6361 cgttccagtc gttcgaattc tccaaagatg gccgcgaggt caccgtcacg gccctgggcc
     6421 gctcatggag gtgccgcatc gagagctaca cgtgcagggc accggagcct gcgcagcccg
     6481 ccggcgggtc gtcggcggcc tcgagcaact cggtcacgtc acccgacggg cggtacgccg
     6541 catacatccg cgacttcaac ctgtgggtca gggacctcgc cgcgaaccag gatcgacagc
     6601 tcacgacaga cggcgtgaag gacttcgggt acgccaccaa caacgcgggc tggatcaaga
     6661 gcgacgcgcc ggtcctgctc tggtcgccgg actcgaagaa gatcgccacg ttccagcacg
     6721 acgaccgcgg cgtcagcgag atgtacctgg tctcgacggc cgtcggcgcg ccgcggctcg
     6781 aagcatggaa gtatccgctg ccgcaggaca aggagatctt caggatccac cgcgtcgtcg
     6841 tcgaggtcga tccggcgcgc gtcgtgcgct tccagatgcc gcccgatcag catcgatcga
     6901 cgatcagcga tcacgtcgcc gagggcagca cgtttctcga cgtgcaatgg tatccggacg
     6961 gcacgcacgt cgctttcgtg tcgtcgtcgc gcgatcacaa ggaggcgatc tttcgtgtgg
     7021 ccgacgcctc gacgggacag gtgcgcaccg tgttcgagga gaagtcccgg acgcagtttc
     7081 agtcggggct ggcagccgtc ggcgagccca actggcgcgt ccgcccgagc gccaacgaag
     7141 tgctgtggtg gtcgcagcgc gacgactggg gacacctgta tctctacgat ctcgagagcg
     7201 gcaggctgaa gcggcaggtg acgagcggca actggaacgt cgcgcagctc gttcacacgg
     7261 acgaaagaag ccgaacgctc tacttcacgg cggtcggccg cgagcggggg cgcgacccgt
     7321 atttccagca tctctatcgc gccgggctcg acggccaggg tgtgacgctg ctgacgcccg
     7381 agcccgcgaa tcacacgatc acgatggcgc ctgatggcca gacgttcctg gacagctact
     7441 cgacgccgac cacgccgccg gtgagcgtgc tgcggcgcat ggacggcgag gtgtcgctcg
     7501 agctcgagcg cgccgacatc tcgcggctgg tcgcggctgg ctggaaacct ccgacgccga
     7561 tcgtcgtgaa ggcgcgcgac ggcaggacgg acctgtatgg gctgatgttc acgccgaccg
     7621 ggctggacga gtcgcgcaag tatccgatcg tcaactacat ctaccccggc ccctggggca
     7681 gcagcgtcgg cggccggagt ttcagtgcgg cgcgcgccga ccatcaagcg ctggccgagc
     7741 tcggattcgt cgttgtcgtg atcgacggca tggggaccga atggcgctcc aagtcgttcg
     7801 ccgacttcta ctacggcgac atcggcgaca acacgctgcc ggatcagatt gccggcatgc
     7861 gcgagctggc gaagcggcat gcctacatcg acatcgatcg cgccggcatc tggggccatt
     7921 ccggcggcgg gttcgcggcc gccgcggcga tgttccggca tccggagttc ttcaaggtgg
     7981 gcgtgtccca ggccggcaat cacgacaacc gcaactacga ggacgactgg ggcgagcggt
     8041 tccagggact cctg
//
LOCUS       DBNGSH010000215         4184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1715318, whole genome shotgun sequence.
ACCESSION   DBNGSH010000215 DBNGSH010000000
VERSION     DBNGSH010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1715318"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="AC16CY_04880"
     CDS             complement(<1..920)
                     /locus_tag="AC16CY_04880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3353147.1"
                     /translation="MRVLLSTIGSRGDVQPLVALALQLKALGADARLCVPPDFRDWIE
                     SLGIPVTPIGPDVRNAGAARRTATATPAPPSPEQLRQVIEATVATQFETVTAAARDCD
                     VIVAATALQIAARSVAEMMGIPYVFAAYCPNVLPSPLHAPPLLPPAPGQSPAPASASN
                     LELWERDAERFNATFGEALNSRRASLGLAPVDNVRRYMFTDRPWLAADPTLAPWPDPS
                     DQAVYQPGAWILPDERPLSAELERFLEAGEPPIYFGLGSVHAPRDVSQVMVDASRALG
                     RRAIVSRGWADLSLVDDERDCFAVDEVNLQ"
     gene            complement(930..1331)
                     /locus_tag="AC16CY_04885"
     CDS             complement(930..1331)
                     /locus_tag="AC16CY_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353148.1"
                     /translation="MRWLHLAVGIVGIAGFLLTGQYMDRVHDHLRGMDDARRLLFRST
                     HIYLLFCSLVNLALGLYFRPGVGWRHWIRLAGSLLVLTAPFVQAIGFFTEPWLSGLER
                     PYTGPAIYACYGGMLLHLIAMSPEKRVTTTE"
     gene            complement(1347..1970)
                     /locus_tag="AC16CY_04890"
     CDS             complement(1347..1970)
                     /locus_tag="AC16CY_04890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3353149.1"
                     /translation="MTDRSNGYEGVAAEFLEGRGRAPSTAIGTTRVRDWARTLPRGAA
                     VIDMGCGTGLPITKVLVTEGLNVYAVDAAPSFVEAFRRNLPGIPVACESVEDSPFFGR
                     TFDGVIAWGLMFLLPPDGQRRLIQRMADVLAPGGRLLFTSEARPLVWNDAMTGLESRS
                     LGGVEYRRLLSEVGLSVSSEYEDEGQNHYYDAIKKGAGGRGQGAGRA"
     gene            complement(1983..2252)
                     /locus_tag="AC16CY_04895"
     CDS             complement(1983..2252)
                     /locus_tag="AC16CY_04895"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR3353150.1"
                     /translation="MEQDLRRVFVASGEMHASQVRAFLEAAGIPTAVRGESLRKTHGL
                     TLDGLGAVEILVADADVEHARSLLDAAEAGQLRLGDDIESSEDAR"
     gene            2373..2717
                     /locus_tag="AC16CY_04900"
     CDS             2373..2717
                     /locus_tag="AC16CY_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3353151.1"
                     /translation="MGDDRLSILKGALDVLILKALSWGPMHGYAVSFWIRSVTNEALE
                     LQEGVLYPALHRLERKGWIDSEWGTSDNNRRAKYYALTKLGRKQLQRELSTWERFANA
                     VGKVVSATEKPA"
     gene            2735..>4184
                     /locus_tag="AC16CY_04905"
     CDS             2735..>4184
                     /locus_tag="AC16CY_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353152.1"
                     /translation="MTRKTPMWRRYVTFWGRDIDGDLREEVEFHIEARTRELVDTGWP
                     PDAAAAEARRQFGNRDAIAAECHVIDVRLEEKKNMKRHLVDLQSDFRFAVRQFVGQPL
                     FSAVAIITLAIGIGSNAAIFSIVDAVLLEPLPYSEPDRLFALRSMESNGLPTGLMAPR
                     FAEPLYEGHRLVEAGALAWSLPGSILSRDRTPYPFKPFRVTGRFFDVFPDVIAMGRTF
                     SPNEPSGSIVISDSTWRNYFGSDANILGSAITVDNSQRTVVGVTRSGFSFPEGAESWE
                     VFDAGPTLADRINFLAYLRLRANATPESLKGDLAVLSTQLGPNAETGKPFTYVLRPLL
                     DEVVGDLSSTVLLLSGATIILLLIACVNVANLLLSRANARSHEVGLREALGAGRLRII
                     RQLMTESLLLCFLGGAFGVALAYVGLRLLLGIGPADLPRLGSVTIDGTVLLFSAGVTL
                     FTTLLIGLAPAFKVSRSRLRQLVDGGGRGASLS"
BASE COUNT          732 a         1361 c         1350 g          741 t
ORIGIN      
        1 tcctgcaggt tgacctcgtc gacggcgaag cagtcgcgtt cgtcgtccac gagggacaga
       61 tcggcccacc ctcgcgacac gatcgcgcgg cgcccgagcg cgcgagacgc gtcgaccatc
      121 acctggctga cgtcccgcgg cgcgtgcacg ctgcccaggc cgaagtagat gggtggctca
      181 ccggcttcaa ggaacctctc cagttcggcg gacaggggac gctcgtcagg caggatccag
      241 gcgccgggct gatacacggc ctggtccgag ggatcgggcc acggcgccag cgtcgggtcg
      301 gcagccagcc acgggcggtc ggtgaacatg taccgtcgca cgttgtcgac tggcgcaagg
      361 ccaagcgagg cccggcggga attcagcgcc tcgccgaagg tggcgttgaa gcgctctgca
      421 tcccgctccc agagctcgag gttgctggcc gatgcgggcg ccggcgactg gcccggcgcc
      481 ggaggcaaca agggcggcgc gtggagcggc gatggcagga cgttcgggca gtaggccgca
      541 aagacgtagg gaattcccat catctccgcc acggagcgcg cggcgatctg cagggcggtt
      601 gccgccacga tgacgtcgca gtctcgggca gccgcggtga ctgtctcgaa ttgagtggcg
      661 accgtggcct ctatcacctg acgcagctgc tccggcgagg gcggggctgg cgtggccgtg
      721 gctgttcgac gcgcagctcc cgcgttgcgc acgtcgggac cgatcggtgt caccgggatg
      781 cccaggctct cgatccagtc ccggaagtcg ggcggcacgc acaggcgggc gtccgcgccg
      841 agcgccttca gctgcagtgc cagcgcgacc agcggctgaa cgtcgcctcg cgacccgatc
      901 gtggacagca gcacgcgcac gtgcatccct tactcggtcg tcgtgacccg tttttccggt
      961 gacatcgcaa tcaagtgcag cagcatgccg ccgtagcagg cgtagatggc cggaccggtg
     1021 tacggccgct cgaggccgga cagccagggc tcggtgaaga acccgatggc ctggacgaat
     1081 ggggctgtca gaacgagaag tgaaccggcc aatcggatcc agtgccgcca cccgacacca
     1141 ggccggaagt acagccccag cgcaaggttg acgagcgagc agaacaacaa atagatgtgc
     1201 gtggaacgaa acaggagccg ccgggcatcg tccatgccgc gcagatgatc gtggactctg
     1261 tccatgtact gcccggtcag gagaaaaccg gcaatgccca cgatgccgac cgccaggtga
     1321 agccatcgca ccggcgcacc tccaactcaa gcccgccctg ccccctgccc cctgccccct
     1381 gcccccttct tgatcgcatc gtaatagtga ttctggccct cgtcctcgta ctcgctggag
     1441 acggagagtc cgacctcgga caggagccgt cgatactcga ctcctccgag cgatcgtgac
     1501 tcgagccccg tcatggcatc gttccacacc agaggcctgg cctccgacgt gaacagcagg
     1561 cggccgccgg gcgcgagcac atcggccatt ctttgaatga ggcgccgctg cccgtcaggc
     1621 ggcagcagga acatcagtcc ccacgcgatc accccgtcga aggtccgtcc aaagaacggc
     1681 gagtcctcga ccgattcgca tgcgaccggt attccgggca ggttgcggcg gaacgcctcg
     1741 acgaaagacg gcgcggcgtc aaccgcatac acgttcaggc cttcggtgac gaggaccttg
     1801 gtgatcggga ggccggtccc gcagcccata tcgatgacgg cggccccacg cggcagcgtg
     1861 cgggcccaat cgcgcacccg tgtcgtgccg atggccgtcg aaggcgcgcg gccgcggccc
     1921 tcgagaaact cggccgccac tccttcatat ccattggatc ggtcggtcat cgacagtcgt
     1981 ggtcagcgcg catcctcgct gctttcgatg tcgtccccga ggcggagctg ccccgcttcg
     2041 gccgcatcga gaagcgaccg tgcgtgctcc acgtcggcat ctgccacgag gatctcgacc
     2101 gccccaaggc cgtcgagcgt gaggccgtgc gtctttcgca gcgattcacc gcggacggca
     2161 gttggaatgc cggcggcctc gagaaacgcg cgcacctggc tggcgtgcat ctcgccgctt
     2221 gccacgaaca cgcgccgaag atcttgctcc atccagcctc ctccggaccc gcccaagggg
     2281 gctcatgatc gcacagatca acgccatcca ccttcccctt gcatcgctat ggaaaatgga
     2341 ttatatccat agctatacaa gggatgatgg cgatgggcga cgaccgactg tccattctga
     2401 agggcgcgct cgacgttctt atcctgaagg cgctgagctg gggccccatg catggctatg
     2461 cagtgtcctt ttggattcgg agcgtcacca acgaagcgct cgagctgcag gagggcgtgc
     2521 tgtatccggc tctccatcgt ctcgagcgca agggttggat cgattcggag tggggaacat
     2581 ccgacaacaa ccgccgcgcg aagtactacg ctctgacgaa gctcggccgc aaacagcttc
     2641 agcgcgagct ctcgacctgg gaacggttcg caaacgccgt aggcaaggtc gtcagcgcca
     2701 ctgaaaagcc tgcgtgactc ctgagttgtg aatcatgacg cgcaagactc cgatgtggcg
     2761 gcgatacgtg accttctggg gacgcgatat cgacggcgat ctccgtgagg aggtggaatt
     2821 ccatatcgag gcccgcacgc gcgagctcgt cgatacaggc tggccgccgg acgcggccgc
     2881 agcggaagca cgccgccagt tcggaaatcg cgacgcgatt gcggcggagt gccacgtgat
     2941 cgacgttcga cttgaagaga agaagaacat gaaacgacac cttgtcgatc tccaaagcga
     3001 cttccggttc gcggtccggc aattcgtcgg ccagcccctg ttttcggctg tcgcgatcat
     3061 cacactggca atcggcatcg gatccaacgc ggccattttc agcatcgtcg atgcggtgct
     3121 gctggagccg ctcccttaca gtgaaccgga tcgcctgttt gcgctccgga gcatggagtc
     3181 gaacggattg ccgacgggcc tgatggcgcc gcgattcgcg gaaccactgt acgagggaca
     3241 tcggctcgtc gaggccgggg cgcttgcctg gtcgctgcca ggctcgattc tgtccaggga
     3301 ccggacgccg taccccttca agcccttccg tgttacgggc cggttcttcg acgtgttccc
     3361 ggacgtcatt gcgatgggac ggaccttttc acccaacgag ccttcgggct cgatcgtcat
     3421 ttccgattcg acgtggcgca actacttcgg gtctgacgcc aacatcctgg gatcggccat
     3481 cactgtcgac aacagccagc gcacggttgt tggtgtgacg cgttccgggt tttcctttcc
     3541 tgagggagcg gaaagctggg aggtctttga tgcagggccg actctggcag accggatcaa
     3601 ttttctggcg tacctccggc ttcgggccaa cgctactccg gagagtctca aaggcgacct
     3661 ggccgtgttg tcgacacaac tggggccgaa cgcagaaacg ggaaagcctt tcacctacgt
     3721 cctgcggccg ctgctcgacg aggtggtggg cgatttgagt tcgaccgtgc tgttgctgtc
     3781 cggcgccacg atcattctgc tcctgatcgc gtgtgtgaac gtggcaaacc tgctgttgtc
     3841 tcgtgccaat gcgcgatcgc atgaggtcgg gctgcgggaa gcattgggcg ccggccgctt
     3901 gcgcatcatc cgtcagctca tgaccgagag cctgctgctc tgcttccttg gcggcgcgtt
     3961 cggcgtggca ctcgcctacg tgggtcttcg actcctgctg ggcatcggcc ctgctgatct
     4021 gccgcgcctc ggatcggtga cgatcgacgg aaccgttctg ctgttttccg cgggcgtcac
     4081 gctgttcacc acattgctca tcgggctcgc tcccgccttc aaggtgagtc ggtctcgact
     4141 gcgtcaactc gtggacgggg gcggccgtgg cgcttcgctg agcc
//
LOCUS       DBNGSH010000216        12948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_356598, whole genome shotgun sequence.
ACCESSION   DBNGSH010000216 DBNGSH010000000
VERSION     DBNGSH010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12948
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_356598"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="AC16CY_04910"
     CDS             <1..217
                     /locus_tag="AC16CY_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3353153.1"
                     /translation="ERAIPAVSQKMLTQQLRRLERDGVVARTVHPEVPPRVEYHLTDW
                     GQSLCPSLDRLLTWAEGQPTTGGPQKP"
     gene            complement(497..1774)
                     /locus_tag="AC16CY_04915"
     CDS             complement(497..1774)
                     /locus_tag="AC16CY_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3353154.1"
                     /translation="MAALNRKMGDEIVIHKHNTARTHRRPSAGLATLTVVALAASAAV
                     NGCGSPGNALGGTPSVAAAEAPTTAETSHALVAEGQKIFRFDTFGDEQVWTDTLRLHE
                     VVEKNVDPTTALKVGLKVDAEVLPPGILATVDLKSPATTVALLKMNAVVGLQATVDSN
                     NHITRLGVTCALCHSNVDDSVMPGIGRRKDGWPNRDLNVGAIIALSPVLSADKKAVYT
                     SWGPGKYDPRYNQDGKNTPLVVPPAYGLALVTNETYTAEGPISYWNAYVAVTQMGGRG
                     NFADPRLGIDVKHSPDMVTSKLPALRAYQHSLLAPPPPAGSVDAAIAARGRVVFDRTC
                     ASCHVGGTGTDNNRGTLHMPAETGVDGAYAARTVSKAYRTTPLRGLWQHAPYFHDGSA
                     ATLADVVTHYNRVRTLGLTTEEQRELTEYLKSL"
     gene            2013..2165
                     /locus_tag="AC16CY_04920"
     CDS             2013..2165
                     /locus_tag="AC16CY_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HIR3353155.1"
                     /translation="MLYTIAVVLLILWLLGLVGTYAIGSVVHVLLVVALVLFVAGLLG
                     GRRSLA"
     gene            2256..2837
                     /locus_tag="AC16CY_04925"
     CDS             2256..2837
                     /locus_tag="AC16CY_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353156.1"
                     /translation="MDLRQLMNPALMALAAAVILAVAILAWYVRRRRRTTTAGLRHRF
                     GTEYELAVGEQGSERRAEAQLEAREKRVEKLRIRDLNPTERERFSVQWQALQSRFVDD
                     PKGAITEADALVSTLMQARGYPVADFSQRAADISVDHPRVVANYRSAHEIALRLGQGE
                     ANTEDLRTGMIHYRSLFDELVHVPTAGAMKEVA"
     gene            2837..3205
                     /locus_tag="AC16CY_04930"
     CDS             2837..3205
                     /locus_tag="AC16CY_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353157.1"
                     /translation="MAEPQTQVPEVTTADLAGWADDGAKPVNGRQLETPPLFSESELG
                     EFRSRWSHVQTGFVDEPRRTVEDADKLVASVMQRLAEGFANERSGLEKQWDQGDHVST
                     EDLRVALQRYRSFFDRLLKL"
     gene            3280..3618
                     /locus_tag="AC16CY_04935"
     CDS             3280..3618
                     /locus_tag="AC16CY_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353158.1"
                     /translation="MSIGIYFAPAAMSAEKYDECITSLRKAGAGHPSGRSYHASFGPK
                     DKLMVFDVWTSQAAFDKFGKTLMPILQQIGIDPGQPSIMPMHKVIRPAARVASPRKRT
                     KVTPARRGKR"
     gene            3776..3919
                     /locus_tag="AC16CY_04940"
     CDS             3776..3919
                     /locus_tag="AC16CY_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353159.1"
                     /translation="MNLLRVAGKSDVVALTPDVSVVSILALVLGVLLLSLGLLLGSTP
                     AAG"
     gene            3994..4920
                     /locus_tag="AC16CY_04945"
     CDS             3994..4920
                     /locus_tag="AC16CY_04945"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3353160.1"
                     /translation="MELGELKVFVTVATEHSFSRAAIKLCRTQPAVSQAVRRLEEDLG
                     ELLFDRSTKSAKLTEAGDALLPEAIRLLRMADEAVATVRQLSQRDRAVLRIGGDEPDA
                     HILLPALSIFLLQQPHVSVEFRRVADLDVPAEVGAGTIDIGLTTRERVPAPLCAVLVP
                     VSSTGFCVLLPKSHPFASLGELPVTVLQSERLVTLADLPLPASITMRATDDAAPPTGL
                     FVVMPGLDSLKHAVANGLGIGIVPRAAVSSLTAAGLVAIPLPAARAASARTLVYRDTD
                     GRRSAVGDFVEALRCTGEDRSRKAPIAMRAAR"
     gene            4917..6614
                     /locus_tag="AC16CY_04950"
     CDS             4917..6614
                     /locus_tag="AC16CY_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3353161.1"
                     /translation="MKLSYVHGVGTTPLIADTVGGALTGAAERWGDRDALVACHQQLR
                     YSYRALRDEADRAARALIALGVQRGDRVGIWSGNRAEWMITQYAAAKAGAVLVNINPA
                     YRLRELEYALTHSGVSVLIAARRFRSTDYVELLLGLMPELMATGTGPLQAQRVPALRH
                     VVYLGTDAEPGGIAWVEFLDRGTRIAAHDLEARETTLQCDDPINIQYTSGTTGSPKGA
                     TLSHHNILNNGYIVGNVLRYTEHDRICLPVPFYHCFGCVLGNMAALTHGSAVVIPGES
                     FDPDATLRAIEAERCTSIYGVPTMFIAQLDHPHFGDVQIETLRTGIMAGAPCPIDVMR
                     QVIGRMHVPQVTIGYGMTETSPLSFQSRIDDPIDIRVSTVGRVLPHLECKIVDPDTGA
                     IVPRGTPGELCTRGYAVMLGYWNDDAATANAIDAARWMHSGDLAVMNDEGYVSITGRI
                     KDMIIRGGENIYPREIEEFLHTHEKVSEVQVIGVPDHKYGEELCAWIRLRDGVTATAE
                     EIREFCRGQIATYKIPRYIRFTSEFPMTVTGKVQKFKIREMTVAELALARVTDVEPT"
     gene            6638..8269
                     /locus_tag="AC16CY_04955"
     CDS             6638..8269
                     /locus_tag="AC16CY_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR3353162.1"
                     /translation="MTRVSWLIPAAILFSNQAAYAQQGCENLVNLKLPYTLITAATSV
                     PESAAPAPAAPGAPPAGPGTVPAHCDVRGVTRPSRDSDIKFALWLPAASAWNGKYRQE
                     GNGGWAGAVNTASFAEPLRRGYAVAGTDNGHEGGGGANWAIGHPEKLIDFGYRAVHET
                     AVQSKAIIRAFYGRDPERSYFNGCSDGGREALMEAQRFPEDFNGILAGAPANNWSRLF
                     TAFVWNERALLATPDSAIPPAKLPVIQRAVMATCDRLDGVADGLIENPRACTVDPAVL
                     VCKGADAADCLPKAQVDALQKIYEGPHNPRTQERLFVGQPPGTEAVPGGWGTWITPAN
                     PAGAIQFMFGNSYYGAAVFEDPNWNFRMLDFDRDVRIGDEKAGPVLNATSPDLRSFRA
                     SGGKLIQYHGWGDAAIPAPSSIEYYESVRAFLSKYPDGRTPGAPPTEDFYRLFLVPGM
                     AHCGGGSGPNSFGNGGGGGAARRDPERDIFTALERWVEQGVAPERLVGTGRATDDPSK
                     PLTRPLCPYPQVAQYRGSGDVNDAANFACAAPSSR"
     gene            8272..9828
                     /locus_tag="AC16CY_04960"
     CDS             8272..9828
                     /locus_tag="AC16CY_04960"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3353163.1"
                     /translation="MTDPNRVLIFDTTLRDGEQAPGCSMTQPEKLRVAKALAELGVDI
                     IEAGFPIASRGDWESVNLVAREVQSPIIAGLARCTREDIDTAWRALKDAARPRIHVFL
                     ATSAIHREHKLNMAKEQIVQTAVEGVTFARSLCDDVEFSPEDASRTELEFLAEVVEAA
                     IDAGATTINVPDTVGYTVPEEFFAVFSYLTRHVRNVDRAVLSVHCHNDLGMAVANSLA
                     AVRAGARQIECTVNGIGERAGNCSLEEAVMAIKTRENVLGVHTGIHTTRLFPTSRLVS
                     QITGMPIPRNKAIVGENAFAHEAGIHQHGMLKHHSTYEIMRPQDVGLSRTNLVLGKHS
                     GRHAFRERVKELGFDVGDAELNQLFEEFKALADKKRELFDGDIEALVMRAANEAAGPW
                     SLQSLSVATHDDQPARVTIGLAHIDGRVVTHDQTADGPVDAVVKAIDHATGIHVQVRT
                     FEVHSVTVGEDAQGEALLTVEHNGRTYRGAGISTNILEAAARAYLEVINRIEAAKASA
                     DRSSSGAAFS"
     gene            complement(10368..12191)
                     /locus_tag="AC16CY_04965"
     CDS             complement(10368..12191)
                     /locus_tag="AC16CY_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353164.1"
                     /translation="MASREGIIRTTIALGIAFTLGAPPTASAQAGQGAGPAPAQQGGG
                     APPAGAQGGGGRGRGGPPPYTPAAGARDLRSVLFNWGWHLGMLRSDQERDIIASLEYQ
                     GKGTMQVGGQPCNVTKYRSSINYQVSGERIQITGTRPNGQSCSTIEVLSGAYAWNEDI
                     PGAELIKGKGKATPMAAATRERMIRLWAGPQGALKSALAGIQDPPLFAVRPGTQVPAD
                     VATAGKTSVVWEGSKPVVSFPIPGVPGATATATLDAKFMAEKVVVRDGTNTIEFTYSD
                     YRDWNNPLHPAEAFVAGRMTERRNGTVVRDITTTVTETGQMYVVMPVPASVKAAITPT
                     LQPPAWVSGFAPPPAPTPSAASQAVTPRLANGKPDMTGSWQGAASGPPGGGNRRCGPT
                     QTRCKVPNDNFVVDYAWISPSRYWMGLNGPVYRPEHWDKIQEMDMWTNKFDPIMTCQP
                     MGIPRQGEPRRIFHTANDITLFYTFSDYGGGNREYRMIPTDGRQHDPNQARQATYLGY
                     SVGKWEGDTLIVDAISFVDSTWLGRGGLFHSGDMRIVEKFTRKGNEILHEMTIHDPES
                     FVEPWVLPARTLTLSNTDALIAERAHCEVYETNDITNQMRH"
     gene            complement(12226..12615)
                     /locus_tag="AC16CY_04970"
     CDS             complement(12226..12615)
                     /locus_tag="AC16CY_04970"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3353165.1"
                     /translation="MNKRTLIAVVLLGLVAASPMLAHHSLAGVYDLNKVERATGVVEK
                     FAFTNPHGALHISIKNAQGEAKVWQLTTGSANVLTNAGVSATGPNRVKHGDEITVTFN
                     PALNGATIGFLRSIVLPDKKEVEFVPN"
BASE COUNT         2315 a         4132 c         4164 g         2337 t
ORIGIN      
        1 cgagcgtgcg attcccgccg tgtcgcagaa gatgctgact cagcaactcc ggcgactcga
       61 gcgcgacggc gtcgttgcac gcaccgtgca tcccgaggtt ccgccgaggg tcgagtacca
      121 ccttaccgat tggggccagt ccctctgccc gtcgctcgac aggttgctga cctgggccga
      181 gggacaaccg acgacgggcg gccctcaaaa gccttgaccg tgaggacccg caaatacgcc
      241 aatgggttat caggccgcat ggatcagcca gttgcggaaa tcgtcccgaa cagccagcca
      301 gctcctttgt cctactccgc gcgcagtgct tcgactgctc tccgctgcgt ccagcacgcg
      361 cgacgtgccg gaggcgcgat gtatcaacga cgcctctatc gtcggtccgg atgaccgttg
      421 aggaggcgcg cgaggcatgc gcccgtgtca tcgccgacgc ggcgtcgaaa aggtcgcagc
      481 aggcgcgcga cgccgatcag agcgacttca gatattcggt gagctcccgc tgctcttcgg
      541 tggtgagccc gagagtacga acgcggttgt aatgcgtcac gacgtccgcg agcgtcgctg
      601 cgctgccgtc gtgaaagtac ggcgcgtgct gccacagtcc acgcagaggt gtggtgcgat
      661 acgccttgct gaccgtgcgc gccgcgtacg ctccgtcaac gccggtttcc gcaggcatgt
      721 gcagcgtccc acgattgtta tctgtgccgg tcccaccaac gtggcagctc gcgcacgtcc
      781 tgtcaaacac cacccgtcca cgcgccgcga tcgccgcatc gacgctgccg gccggaggtg
      841 ggggcgccag caagctatgt tggtacgcgc gcaacgcggg gagcttggag gtaaccatgt
      901 ccggcgagtg cttgacgtcg atcccgagcc gcgggtcggc gaaattacct cgtccgccca
      961 tctgggtgac ggcgacatag gcattccaat aggagatcgg cccttcggcc gtgtacgttt
     1021 cgttcgtgac cagtgccagg ccataggcgg gcgggacgac gagcggcgtg ttcttcccgt
     1081 cctggttgta cctcgggtcg tatttgcccg ggccccacga cgtgtagacc gctttcttgt
     1141 ccgcggagag caccggcgac agcgcaatga tggccccgac gttcagatct cggttcggcc
     1201 acccgtcttt cctgcgcccg atgccgggca tcaccgaatc atccacgttg gagtgacaga
     1261 gcgcgcaggt gacaccgagc cgcgtgatgt ggttgttgga gtccacggtg gcctggaggc
     1321 ccacgacggc attcatcttg agcagcgcga ccgtcgtcgc cgggctcttg agatcgacgg
     1381 tggcgaggat ccccggaggc agcacctcgg catccacttt gagtccgacc ttgagtgctg
     1441 tcgtcgggtc cacgttcttc tcgacgacct cgtggagccg gagcgtgtcg gtccagactt
     1501 gctcgtcacc gaaggtgtca aagcggaaga tcttctggcc ctcggcaacg agcgcgtgtg
     1561 atgtctccgc ggttgtcgga gcttcagcgg ccgctacgga cggcgttccg ccaagagcgt
     1621 tgcccggcga tccgcagccg ttgactgctg cgctggcggc caacgcgacg acggtcagcg
     1681 ttgccagacc agcgcttggg cgtcgatgcg tgcgagctgt gttgtgtttg tggatcacga
     1741 tttcgtcccc catcttccga ttcagggcag ccacgtgagc gctcccgtga cctcgaagag
     1801 aaggccaggg gaatcccatt acaacaacgc agatgtgtcg tcgtctgtcc aaaaccacac
     1861 acggtgcgtg acaccacgcg acggcggcac caccatgtgc acctgtactc aaacgtacag
     1921 acacgcgtca gcgcttgccg catgctgggt gcgccgcgga tcgctccccc gatcgtgcgg
     1981 ttgtgaagcg aactcggaat ggagagattt caatgctgta caccatcgcc gtggttctac
     2041 tgatcctgtg gctgctgggc ctcgtcggga catacgcgat cggctcggtt gtccatgtgc
     2101 tcctggtcgt cgccctcgtg ttgttcgtcg ccgggctgct gggcggtcgc cgatcgctgg
     2161 cctgatccac acgccagaga cgcggagtgc atcagccagc tggcggacac gcctcttcag
     2221 cttgacaacg atattcgtga tgcagggagg tcttgatgga cctgagacaa ctgatgaacc
     2281 ccgccctgat ggcgctcgcc gctgcagtca tcctggccgt cgcgatcctt gcttggtacg
     2341 tgcgaagacg gcgtcgaacc acgacggccg ggttgcgcca ccgttttggg acggaatacg
     2401 aactggcggt aggcgagcag gggtctgaac gccgtgcaga agcgcaactg gaggctcgtg
     2461 agaagcgggt tgagaaactc cgtatccgcg atctgaatcc gacggagcga gaacgcttct
     2521 ctgtccagtg gcaggcattg cagtcccgct tcgtcgatga cccgaagggg gcgattacag
     2581 aggcggatgc actcgtctct accttgatgc aggcccgtgg ctatcctgtc gccgatttca
     2641 gtcaacgtgc cgcggatatc tccgtcgacc atccccgagt ggtggcgaat taccggtccg
     2701 cgcacgagat cgccttgcgg ctcggtcaag gtgaagccaa cacagaagac ttgcgaaccg
     2761 ggatgatcca ttaccgctcc ctcttcgacg agctcgtgca cgtaccaacc gccggggcaa
     2821 tgaaagaggt ggcatgatgg ctgagccgca aacacaagtg cctgaagtca cgacggccga
     2881 cctcgcgggc tgggccgatg acggcgcgaa gccggtcaac gggcgacaac tggagacgcc
     2941 gccgttgttc tctgaatcgg aactgggaga gttccgatca cgctggagtc acgttcagac
     3001 ggggttcgtg gacgagccgc gtcggaccgt cgaagacgct gacaagcttg tggcgtcggt
     3061 gatgcagcga ctcgcagaag ggttcgcgaa cgagcggtcg gggctggaga agcagtggga
     3121 ccagggcgac cacgtgtcga cggaagactt gcgggttgcc ctgcagcgct atcgttcatt
     3181 ctttgacaga ttgctgaaac tgtagtccag cgtctcgtcg ccggcgcgaa ggcaacgcgc
     3241 tcccgagagt gcggcgggcc catgaaggag gtcgccacta tgtcgattgg gatctatttc
     3301 gcaccggccg ccatgtcggc ggagaagtac gatgagtgca tcacgtcatt gcggaaggct
     3361 ggggccggtc acccgtcggg gcggtcgtat cacgcgtcgt tcggcccgaa ggacaaactg
     3421 atggtgttcg acgtatggac atcgcaagcg gcgttcgaca agttcgggaa gacgctgatg
     3481 cccatcctgc agcaaatcgg gatcgatccc ggtcagccga gcatcatgcc aatgcacaag
     3541 gtgatccggc ccgcggcaag agtcgcatcg ccccggaagc gcacgaaggt gacgccggca
     3601 cgccgtggga agcgctaggc tagcgcgcgc tcggccgcgg ttacacgtgc gctgtcgtct
     3661 ccgggtaagc ccgggggtcg agacagttcg agaccggcat aaccaccgga cgcagcgccc
     3721 gaacaatggg cggacggttg acacatcaag ctggcacggg cgatactccg cctccatgaa
     3781 cctgctgagg gtcgccggca agtccgacgt cgtggcgctg acgcccgacg tgtcggtcgt
     3841 gtcgatcctc gcactggtgc ttggcgtcct cctacttagc cttgggctgc ttctgggaag
     3901 cacccccgcc gccggatgat gtagttcaca agtttcagtg caccaagggc catcatccga
     3961 caagggtgat ggcccttttg cttttgaggg tctatggagc tcggagaact caaagtcttc
     4021 gtcacagtcg cgaccgaaca cagcttctcg cgggccgcca tcaaactctg tcgaacccag
     4081 ccggcggtga gccaagccgt ccgccgcctt gaagaggatc tgggcgagct cttgttcgat
     4141 cgcagcacga agtccgcgaa gctcacggag gcaggcgacg cgctgctgcc cgaagcgatt
     4201 cgactgctcc gaatggccga cgaagcagtg gccacggtcc ggcaactgtc gcagcgggac
     4261 cgcgcggtcc tgcggattgg cggcgacgaa cccgacgctc acatcctgct cccggcgttg
     4321 tcgatattcc tcctccagca gccgcacgtc agcgtcgaat ttcggcgcgt ggccgacctg
     4381 gacgtccccg cggaagtcgg agcagggacc atcgacattg gcctcacgac cagggaacgt
     4441 gtgcccgcgc cgttgtgtgc cgtgctggta cccgtctcgt ctaccggctt ctgcgtcctg
     4501 ctgccgaaga gccacccctt tgcgtcgttg ggcgagctgc cggtgacggt cctccaatcc
     4561 gaacggctcg tgacgctggc tgatctgcca ctgccggcgt cgatcacgat gagagccacc
     4621 gatgatgccg cgcctcccac gggcctattt gtcgtcatgc cgggcctcga cagtctgaaa
     4681 cacgcggttg ccaatgggct tggcatcggg atcgttccac gcgccgcagt gtcgagcctc
     4741 accgctgccg gactggtcgc gatccccctg ccggcggcgc gggcagccag cgcgcgcacg
     4801 ctcgtgtatc gcgacaccga cggacgacga agtgctgtcg gggacttcgt cgaggccctg
     4861 cggtgcaccg gggaggaccg ctcccgaaaa gccccgatcg ccatgagggc cgcgaggtga
     4921 agctctccta cgttcacggc gtcggcacca cgcctctgat cgcggatacc gtcggcggcg
     4981 ccctcactgg tgcggccgaa cgctggggtg accgcgacgc gctcgtggca tgtcatcagc
     5041 agctgcgcta ttcgtaccga gcgcttcgcg acgaggccga ccgcgccgcc cgcgcgctga
     5101 tcgcgctcgg ggtgcaacgc ggcgaccgtg tcggcatctg gagcggcaac cgcgcggagt
     5161 ggatgatcac gcaatacgcg gcggcaaaag caggcgccgt cctcgtgaac atcaatccgg
     5221 cctaccgcct gcgagagctg gaatacgcgc tgacccactc gggcgtgagc gtgctcattg
     5281 ccgcccgtcg gttccgctcg accgactatg tcgaactgtt gctggggctc atgccggaac
     5341 tgatggcgac ggggaccggg cctctccagg cgcaacgggt gccggcgctt cgccatgttg
     5401 tttacctggg taccgacgcg gaaccgggcg ggatcgcctg ggtagagttc ctggatcgcg
     5461 gcactcgaat cgcggcgcac gatctcgagg cgcgcgaaac gacgctgcag tgcgatgatc
     5521 cgatcaacat tcagtacacc tccgggacga cagggtcgcc gaaaggggcg acgctctcgc
     5581 accacaacat tctgaacaac ggctacatcg tcggcaacgt gttgcgttac acagaacacg
     5641 acaggatttg cctgcctgtc ccgttctatc actgcttcgg ctgcgtgctt ggcaacatgg
     5701 cggccctcac gcacggcagc gccgtggtca tccctggtga gtcgttcgat ccggacgcca
     5761 cgctgcgggc gatcgaggcg gaacgctgca cgtccatcta tggcgtaccg acgatgttca
     5821 tcgcacagct ggaccatcct cacttcggcg acgtccagat cgaaacgctg cgcaccggaa
     5881 tcatggctgg cgcgccatgt ccgatcgacg tcatgcggca ggtcattggc cgcatgcacg
     5941 tgccgcaggt caccatcggc tatggcatga ccgaaacgtc cccgctgtcg ttccagtccc
     6001 ggatcgacga tccgattgac attcgcgtgt cgacggtcgg ccgcgtgctg ccacacctcg
     6061 aatgcaagat cgtcgatccg gacaccggcg cgatcgttcc gcgcggcacc ccaggagagc
     6121 tgtgcacacg cggttacgct gtgatgctcg ggtactggaa cgacgacgcc gcaaccgcca
     6181 acgcgattga cgcggcgcgc tggatgcaca gcggcgacct cgccgtcatg aacgatgagg
     6241 gctacgtgtc gatcaccggc cgcatcaagg acatgatcat ccgcggcggt gagaacatct
     6301 atccccgtga aatcgaagag ttcctgcaca cccacgaaaa agtcagcgaa gtgcaggtga
     6361 ttggtgtgcc cgatcacaag tacggcgaag agttgtgcgc gtggatccgg ctgcgtgacg
     6421 gagtcaccgc aacggcggag gagatccgcg agttctgtcg gggccagatc gccacctaca
     6481 agattccgcg gtatatccgg ttcaccagcg aatttccaat gaccgtgacc ggaaaggttc
     6541 agaaattcaa gattcgggag atgaccgtgg ccgagctggc cctcgcccgt gtgaccgatg
     6601 tggagccgac ctgaggtcgg cgagaggaga gaacgtgttg acaagagttt cgtggctcat
     6661 accggcggcg atcctgttca gcaaccaggc tgcgtatgcg cagcaagggt gcgagaacct
     6721 ggtgaacctg aagctgccgt atacgctcat caccgcggcg acgagcgtgc cggaaagcgc
     6781 cgcaccggcg cccgccgctc cgggtgcgcc accggcgggt ccgggcacgg tacccgccca
     6841 ttgcgacgtt cgcggcgtca cacgtccaag ccgcgattcc gatatcaagt ttgccctgtg
     6901 gctgccagca gcgtccgcct ggaacggcaa gtaccggcag gaaggcaacg gcggctgggc
     6961 aggcgccgtc aacaccgcct cctttgcaga gcccttgcgc cgcggctacg ccgtcgccgg
     7021 caccgacaat ggccatgaag gtggcggcgg cgcgaactgg gccatcggac atccggagaa
     7081 actgatcgac tttgggtacc gcgccgtgca tgagaccgcg gtgcagtcca aggcgatcat
     7141 ccgcgcgttc tacggccgag acccggagcg gagctacttc aacggctgct ccgacggcgg
     7201 ccgcgaagcg ctgatggagg cgcagcgctt tccggaagac ttcaacggca tcctggccgg
     7261 cgccccggca aacaactggt cgcgcttgtt caccgcgttc gtctggaacg agcgcgcgct
     7321 cctggccacg ccggacagcg cgatccctcc cgcgaaactc ccggtcatcc agcgcgccgt
     7381 catggccaca tgcgatcgtc tcgacggcgt ggccgacggc ttgatcgaga atccgcgtgc
     7441 ctgcacggtc gatccggccg tgctcgtctg taaaggagcg gacgcggccg attgtctgcc
     7501 caaagctcag gtcgatgcgc tgcagaagat ctacgaggga ccgcacaacc cgcgcaccca
     7561 ggaacgcctc ttcgtcggac agccgcccgg cacggaagcg gtgcccggcg gctggggtac
     7621 ctggatcacg cccgcgaacc ccgcaggtgc catccaattc atgttcggca acagctacta
     7681 cggggcggcg gtattcgagg accccaactg gaatttccgg atgctcgact tcgatcgcga
     7741 cgtgcgcatc ggcgatgaga aggcgggacc ggtcctcaac gcgaccagcc ccgacctgcg
     7801 gtcattccgt gcgtcaggcg ggaagctcat tcagtaccac gggtggggag acgccgccat
     7861 tcccgcgccg agttcgatcg agtactacga gagcgtgcgc gcctttctct cgaaataccc
     7921 ggatgggcgg acgcctggcg ctccgccgac cgaggatttc taccggctgt ttctcgtgcc
     7981 tggcatggcg cactgcggtg gcggctcggg cccgaacagt ttcggcaacg ggggcggcgg
     8041 aggggctgcc cgccgggatc ctgagcgcga tatcttcact gctctcgagc gatgggtcga
     8101 gcagggcgtc gcgccggaac gcctggtcgg taccggacgc gctaccgatg atccgtccaa
     8161 gccgctcact cggccgctgt gcccctatcc gcaggtggcg caataccgcg gctcgggcga
     8221 cgtgaacgac gcggcgaact tcgcctgtgc cgcgccatcg tcgagataac gatgaccgac
     8281 cccaaccgcg tcctcatctt cgacacgacg ctgcgcgacg gcgagcaggc tcccggctgc
     8341 agcatgacgc agccagagaa gctgcgggtc gcgaaggcgc tcgcggagct cggagtcgac
     8401 atcatcgaag ccggtttccc gatcgcctcg cgcggcgatt gggagtcggt gaatctggtg
     8461 gctcgcgaag tccagagccc gatcatcgcg ggtctcgcgc gctgcactcg agaagacatc
     8521 gacaccgcgt ggcgcgcgtt gaaggatgcc gcgcgtccgc gaatccacgt gttcctcgcc
     8581 acgagcgcca ttcatcgcga acacaaactc aacatggcga aggagcagat cgtccagacg
     8641 gcggtcgaag gcgtgacgtt tgcacgcagt ctctgcgacg acgtggaatt ctcgccggaa
     8701 gacgcctcgc gcacggaact cgagtttctc gccgaggtcg tcgaagccgc catcgacgct
     8761 ggcgccacca cgattaacgt ccccgatacc gtcggctata cggtgccaga agagttcttc
     8821 gcagtgtttt cgtacctcac acgccacgtg cgcaacgtcg acagagccgt gctctccgtt
     8881 cactgccaca acgacttggg gatggcggtc gcaaacagcc tggcggcggt acgcgccggg
     8941 gcacggcaaa tcgagtgcac ggtcaacggc atcggcgaac gtgccggcaa ctgctccctc
     9001 gaggaagcgg tcatggcgat caagacccgc gagaatgtgc tcggcgtgca caccggaatt
     9061 catacgaccc gcctgtttcc aaccagtcgc ctggtgtcgc agatcaccgg catgcccatt
     9121 ccgcgcaata aggccatcgt cggtgaaaac gcgttcgcgc acgaagccgg cattcatcag
     9181 cacggcatgc tcaagcatca ctcgacctac gagatcatgc ggcctcaaga tgtggggctg
     9241 tcgcgcacca acctcgtgct cggcaagcac agcggccgac acgcgtttcg cgagcgtgtc
     9301 aaggagctcg gtttcgacgt cggcgacgcc gaactcaatc aactgttcga agaattcaag
     9361 gcgttggccg acaaaaagag ggagctcttc gacggcgaca tcgaagcgtt ggtcatgcgc
     9421 gcggccaacg aagccgcggg gccgtggtcg ttgcagtctc tgagcgtcgc cacccacgac
     9481 gatcaaccgg cgcgcgtcac gatcgggctc gcgcacatcg atgggcgtgt cgtcacccat
     9541 gatcaaaccg ccgacggacc cgtcgacgcc gtggtcaaag ccatcgacca tgccaccgga
     9601 attcatgtcc aggtgcgcac gttcgaagtg cacagcgtga ccgtcggcga ggatgcacaa
     9661 ggcgaagcac tgctcaccgt tgaacacaac ggtcgcacct atcgcggcgc gggcatcagc
     9721 accaatattc tcgaagccgc cgcgcgggcg tacttggaag tcatcaatcg catcgaagcc
     9781 gcgaaggcca gcgctgaccg aagcagtagc ggcgccgctt tcagttgagc ccgacgctcg
     9841 ggcccggcgt gtcctttcag cgcccgacca tcttctgcaa ctgctcagta cgtgcagaga
     9901 cggcgtcgaa ccacgacggc cgggttgcgc cggcgttttg ggccggaata cgaactggcg
     9961 gtgggcgagc tagggtctga acgccgagcg gaagcgcaac tggaggctcg tgcgctatcg
    10021 ttcattcttt gacagattgc tgaaactgag tgccgcgtct cgtcgccggc gcgaaggcaa
    10081 cgcgctcccg agagtgcggc gggcccatgg ccgagcatca tgccgatgca caaggtgatc
    10141 cggcccgcgg caagagtcgc atcgccccgg aagcgcgcga aggcgacgtc ggcacgccgc
    10201 cggaggcgct aggctggttg ccatctggat cgagcaggcc gtcaaggcgg tgtccgagat
    10261 ggaagcgtct gtcgaggcgc ggtgggactg accaacgcct ggggaattgc gggacgccga
    10321 tcgagatcga tcagcgtccc tttgtctctg tggcgattct tcgcgagcta gtgtcgcatc
    10381 tgattggtga tgtcgttcgt ttcgtagacc tcgcaatgcg cacgctccgc aatgagtgcg
    10441 tcggtgttgc tgagagtcag cgtgcgcgcc ggcaggaccc agggttcgac aaacgactcg
    10501 gggtcgtgga tggtcatttc gtgcaggatt tcattgccct tgcgcgtgaa cttttcgacg
    10561 atgcgcatgt cgccggaatg aaagagaccg ccgcgtccca gccacgtgga atcgacgaac
    10621 gaaatggcgt cgacgatcag cgtgtcgccc tcccacttgc ccacggagta gccaagatag
    10681 gtcgcctgtc tcgcctggtt cggatcgtgc tgacggccgt cggtcgggat catgcgatat
    10741 tccctgtttc cgccgccgta atcgctgaac gtatagaaca aggtaatgtc gtttgccgtg
    10801 tggaagatgc ggcggggctc gccctggcgc ggaatcccca tcggctggca ggtcatgatg
    10861 ggatcgaact tgttcgtcca catgtccatc tcttggatct tgtcccagtg ctcgggtctg
    10921 tacaccgggc cgttcaggcc catccaatac cgcgagggcg agatccacgc atagtccacc
    10981 acgaagttgt cgttcgggac tttgcagcgg gtctgcgtcg ggccacagcg gcggttcccg
    11041 ccacccggtg ggcctgacgc ggccccctgc caactccccg tcatgtctgg cttgccgttg
    11101 gcgagcctcg gcgttacggc ctgagaagcc gccgatggcg ttggcgccgg tgggggtgcg
    11161 aatcccgaga cccaggcagg aggctgcagc gttggcgtga tcgccgcttt gacgctggcg
    11221 ggcacgggca tcacgacata catctgccct gtttccgtca ccgtggtcgt gatatcgcga
    11281 acgaccgtcc cattccggcg ttcggtcatc ctgccggcga caaacgcctc ggccgggtgg
    11341 agcgggttgt tccagtcccg atagtcgctg taggtgaact cgatggtgtt cgttccgtcc
    11401 cgcaccacga ctttttcagc catgaacttc gcgtcgagcg tcgctgtcgc ggtcgccccc
    11461 gggacgccag ggatcgggaa tgacaccacg ggcttgctac cctcccacac aaccgacgtc
    11521 ttcccggccg ttgcaacatc ggcgggtacc tgcgtgcccg gtctgaccgc gaagagcggc
    11581 ggatcctgga tccccgccag cgcggatttg agcgcgccct gcggaccggc ccacaggcga
    11641 atcatccgct ctcgggtggc tgccgccatc ggcgtcgctt tgcctttgcc cttgatcagt
    11701 tccgccccag ggatatcttc gttccacgcg taggccccac tgaggacttc gatcgtggaa
    11761 caactctggc cgttgggccg cgtgccggtg atctgaatcc gttcacccga cacctggtag
    11821 ttgatgctgc tccggtactt cgtcacattg cagggctgcc cacccacttg catggtgccc
    11881 ttcccctgat actcgagcga cgcgatgatg tcgcgttcct gatcactgcg cagcatgccc
    11941 agatgccagc cccagttgaa gagcaccgag cgaaggtccc gggcgcctgc cgcgggagtg
    12001 tacggtggtg gaccgcccct gccacggcct ccgccgccct gtgcaccggc cggtggagcg
    12061 cctccgccct gttgtgcagg ggcggggcct gctccctgac ccgcctgtgc gctggcagtc
    12121 ggcggggcgc cgagcgtgaa cgcgattccc agcgcgatag tggtacggat tattccttct
    12181 cgagacgcca tcactagtct cctatcgtca gctcaaaccg cgcggtcagt tcggtacaaa
    12241 ctcgacttct ttcttgtccg gcaacacgat cgatctgaga aaaccgatgg tggcgccgtt
    12301 gagcgcggga ttgaaggtga cggtgatctc atcgccgtgc ttgacccgat tcgggcccgt
    12361 ggcgctgacc ccggcgttgg tcaggacgtt ggcagaaccc gtcgtcagct gccacacctt
    12421 ggcctcgccc tgcgcgttct tgattgaaat gtgcagcgcg ccgtgggggt tcgtaaacgc
    12481 aaacttctca accacgccgg tcgcccgttc taccttgttc aagtcgtaca cgccggccag
    12541 ggaatgatgc gccagcatcg gactggcagc taccagcccc aataagacga cagcgatgag
    12601 agtccgcttg ttcatcgtga gcctccgaag attttgccga acggaaacag gatatttctg
    12661 ccgggggagt gtacctccgg tttcctcgca gatctaccgt acggaggatg aacggaaagg
    12721 ggggcgggat gaacctggca ccccggcctg ccggccggga tgcccatggt ttagcgtgag
    12781 cctgctagtc gtagccagcg agcgaagcga gcggtttggc gaaggctagt gtctacggaa
    12841 cgccttaacg gcgttcctta gtgccgcagc tgattggtga tgctttgctc ctcgtagatc
    12901 tcgcaatggt tacgctccgg aatcagggcg tcgccggtgc cgagagtc
//
LOCUS       DBNGSH010000217         3713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4432090, whole genome shotgun sequence.
ACCESSION   DBNGSH010000217 DBNGSH010000000
VERSION     DBNGSH010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4432090"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="AC16CY_04975"
     CDS             complement(<1..1023)
                     /locus_tag="AC16CY_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3353166.1"
                     /translation="MRFLKNKRLIASVLVVAAIVAAALWPQSMEVTVARAVRGPMQVT
                     VDEDGETRVRDRFTISAPVSGRVQRIEIEPGDPVVRGTTVLARITPAASPLLDPRTSG
                     ELKAAVDAATAAVGQARAERQRAATTLERARSLLRRQQELLKVGAVASDTLEDAETAV
                     ATGEEAVRAAQFTEQRAEYEVQLARARLAAPSASGNAVHIVSPVNGVVLKRYRESESV
                     VPVGDPLLEIGEPGRMEIVADLLSTDAVRVMPGADVLIEQWGGGHTLHGRVKRVEPSG
                     FMKVSALGVEEQRVNVVVDFTDLAEAARLGDGYRVEVRVVLWQEKDVVKVPVGSLFRQ
                     GEDWAVF"
     gene            complement(1103..3472)
                     /locus_tag="AC16CY_04980"
     CDS             complement(1103..3472)
                     /locus_tag="AC16CY_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353167.1"
                     /translation="MSLSALNRKLLRDLWAMKGQAFAIALVVAAGVAMFVMYQSNFDS
                     LWETRRAYYERQRFGHVFASLKRAPERLAADIAAIPGVSAMETRVVSAVTLDLEQLDE
                     PAGAQLVSIPANRRPRVNDLFLRRGRWVEPGRPDEILASEGFVVAHGLQLGDQVPALI
                     NGRLRRLTIVGVALSPEYVYSIRPGELVPDDKRYGIFWMDKRALAAAFDMEGGFNDVT
                     LAVAPGASTEEIISRLDRLLEPYGGLGAIPRALQLSHWAVENELSQLQSFGFLLPLIF
                     LIVASFILNVALTRALALQRSQIASLKALGYDNLSLGWHYLKWALVIGAVGVVIGVAA
                     GVALGTMIIELYNVYFRFPELAFNVTLRVVVGSAALTIAAAGAGALGAVVRAVKIPPA
                     EAMRPEAPARYRRTVLETPLISRHLGTAGRMVLRNIARHPFRASASIFGISFAVAILM
                     IGLVFTDAIEQLILTQFFEAERQDVTVNFVEPRGDDARHALARLPGVIAVEPQRTVAA
                     RVRAGHRERYLAISGVPRVPRFRRIVDRNGEPIRLPPGGVVLSLTLANVLGVKPGDMV
                     TMDVLEGRRPTRQIEVSGLVDDLLGLSAYMDIDALHQMMQESDVITGALMLVDSSEEP
                     RLATTLKATPAVAGTGFKRAVLRSFREVLAANMGLSIFINLLFAGVIAFGVVYNAARI
                     SLSERSRELASLRVLGFTRAEISLILMGELALLTLVSLPIGALLGYGLAALIVGSIDS
                     EVYRFPLYVSREAVAWSFLGIIAAALISGLIVRRMLDKLDLVAVLKIKE"
     gene            complement(3529..>3713)
                     /locus_tag="AC16CY_04985"
     CDS             complement(3529..>3713)
                     /locus_tag="AC16CY_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353168.1"
                     /translation="GIVVLEALQRVNADLGTTTVVITHNAAIASMADRVIRLADGRVA
                     SVEERTDKLAPSELSW"
BASE COUNT          641 a         1313 c         1224 g          535 t
ORIGIN      
        1 gaacaccgcc cagtcctcgc cttggcggaa cagcgacccc acgggcactt tcaccacgtc
       61 cttctcctgc cacagcacca cgcggacctc gacgcggtag ccgtcgccga gcctggccgc
      121 ttccgccaaa tcggtgaagt ccacgacgac gttgacgcgc tgctcctcga cgccgagcgc
      181 cgacactttc atgaagcccg agggctcgac gcgcttgacg cgcccgtgga gcgtgtgccc
      241 gccgccccat tgctcgatca gcacgtccgc gcccggcatg acgcgcacgg catcggttga
      301 cagcagatcg gccacgatct ccatccggcc cggctcgccg atctcgagca gcggatcgcc
      361 gacgggaacg accgactcgc tttcgcggta gcgcttgagc acgacgccgt tgaccggcga
      421 cacgatgtgg acggcgtttc ccgacgcgga cggcgccgcc agccgggcgc gcgcgagctg
      481 cacctcgtac tccgcccgct gctccgtgaa ctgggccgcg cgcaccgcct cctcgccggt
      541 ggcgacagcg gtctcggcgt cctccagcgt atcgctggcg accgcgccca ctttcagcag
      601 ctcctgctgg cgccggagca gcgatcgcgc gcgctcgagc gtcgtcgcgg cgcgctggcg
      661 ctcggcccgc gcctgtccga ccgccgccgt cgccgcgtcc accgcggcct tcagctctcc
      721 gctcgtacgc ggatcgagca gcggcgacgc ggccggcgtg attcgtgcca ggaccgtcgt
      781 gccgcggacc accgggtcgc ctggctcgat ctcgatgcgc tgcacgcggc cgctcaccgg
      841 ggcggagatc gtgaatcgat cgcgcacccg cgtctcgccg tcctcgtcga cggtaacctg
      901 catcgggcca cggacggcgc gcgcaaccgt gacctccatc gactggggcc acagggcggc
      961 ggcgacgatc gcggcgacga cgagcacgga ggcaatcaac cgtttgttct tcagaaagcg
     1021 cacgcaacct ccgtaaaaat ggggaatggg gtcgggagtc attttccctc agtgaagtaa
     1081 gcggaaaatg actcccgacc ccttattcct tgatcttcag tactgcgacc aggtcgagct
     1141 tgtccagcat gcgcctgacg atcaggcccg agatgagcgc cgcggcgatg atgcccagaa
     1201 acgaccaggc gaccgcctcc cgcgacacgt agagcggaaa ccggtacacc tcgctgtcga
     1261 tcgatccgac gatgagcgcg gccaggccgt atcctaacag cgctccgatt ggcagcgaca
     1321 cgagcgtcag cagcgcgagc tcgcccatca ggatgagaga gatctccgcg cgggtgaagc
     1381 ccaggacccg gaggctcgcc agctcgcggc ttctctccga cagcgagatg cgcgcggcgt
     1441 tgtacaccac gccgaatgcg atcacgcccg cgaagagcag gttgatgaag atcgacagac
     1501 ccatattggc cgcgagcacc tcgcggaagc ttctaagcac ggcccgcttg aagccggtcc
     1561 cggcgaccgc gggagtggcc ttgagcgtcg tcgcgaggcg gggctcctcc gacgagtcga
     1621 ccagcatgag cgcgcccgtg atcacgtcgc tctcctgcat catctggtgc agcgcgtcga
     1681 tgtccatgta cgccgacagg ccgagcagat cgtcgacgag ccccgacacc tcgatctgcc
     1741 gagtcggacg tcggccttcg agcacgtcca tcgtgaccat gtcgcccggc ttgacgccca
     1801 gcacgttcgc cagcgtgagc gacagcacga cgccgcccgg cggcaggcgg atcggctcgc
     1861 cgttgcgatc gacaatccgc cggaaccggg ggactcgagg gactccgctg atcgccagat
     1921 agcgctcgcg atggccggcg cgcacgcggg cggccaccgt ccgctgaggc tccacggcaa
     1981 tgacgcccgg cagccgcgcg agcgcgtgcc gggcatcgtc tccgcgcggc tcgacgaagt
     2041 tgacggtgac gtcctgccgc tcggcctcga agaactgcgt caggatcagc tgctcgatcg
     2101 catccgtgaa caccaggccg atcatcagga tggcgaccgc gaacgagatg ccgaagatgg
     2161 acgccgaggc gcggaacggg tggcgcgcga tgttccggag caccatgcgg ccggcggtgc
     2221 ccaggtgcct cgagatgagc ggggtttcga gcacagtccg tctgtagcgc gcgggtgctt
     2281 cgggtcgcat cgcctcggcc ggggggatct tcacggcgcg aacgacggcg cccagcgcgc
     2341 ccgcgcctgc cgcggcgatc gtcagtgccg cggagccgac gacgacccgc agcgtcacgt
     2401 tgaaggcgag ctccggaaag cggaagtaga cgttgtagag ctcgatgatc atcgtgccga
     2461 gggccacgcc cgcagcgaca ccgatgacaa cgcccacggc tccgatgacg agcgcccact
     2521 tcaagtagtg ccacccgagc gaaagattgt cgtagccgag ggccttgagc gaggcgattt
     2581 gagaccgctg cagggcgagc gcgcgcgtca gggccacgtt gaggatgaag gaggcgacga
     2641 tcagaaagat gagcggcagc aggaacccga agctctggag ctgggacagc tcgttctcca
     2701 ccgcccagtg cgagagctgg agcgcacgcg gaatagcgcc aagccccccg tacggctcga
     2761 gcaggcgatc gagcctcgag atgatttcct cggttgacgc ccccggagcg acggcgagcg
     2821 tgacgtcgtt gaagccaccc tccatgtcga aggcggcggc cagcgccctc ttgtccatcc
     2881 agaagatccc gtaccgcttg tcgtcgggca ccaactctcc cggcctgatg ctgtagacgt
     2941 actcgggtga cagggcgacg ccgacgatcg tcaggcggcg caggcggccg ttgatcagcg
     3001 cgggaacctg gtcgccaagt tggagtccgt gcgccacgac gaatccctcg ctggccagga
     3061 tctcgtcggg ccgcccgggc tcgacccagc gtccgcgacg gagaaagagg tcgttgacgc
     3121 gagggcggcg gttggccgga atcgacacga gctgcgcgcc cgcgggctcg tcgagctgct
     3181 cgaggtcgag cgtcaccgcg gagacgacgc gcgtttccat cgcggacacg ccgggtatcg
     3241 ccgcgatgtc ggcggccagc cgctcgggcg cgcgcttgag cgaggcgaag acgtggccga
     3301 atcgctgccg ctcgtagtag gcgcggcgcg tctcccacag cgagtcgaag ttcgactggt
     3361 acatgacgaa catcgccacg ccagccgcca cgaccagcgc gatggcgaac gcctgcccct
     3421 tcatggccca caggtcgcgg agcagcttcc ggttcagcgc cgacaggctc acgccttaac
     3481 tcccaactcc caaatcccaa ctcccacact cagactcaca actcgaactc accaggacaa
     3541 ctcgctcggg gcgagcttgt ccgtccgctc ctcgaccgac gccacgcggc cgtccgcgag
     3601 ccggatgacg cggtcggcca tcgaagcgat cgccgcgttg tgcgtgatca cgaccgtcgt
     3661 cgtccccagg tccgcgttga cgcgctgcag cgcctcgagc acgacgatcc ccg
//
LOCUS       DBNGSH010000218         3330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2564277, whole genome shotgun sequence.
ACCESSION   DBNGSH010000218 DBNGSH010000000
VERSION     DBNGSH010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2564277"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..913
                     /locus_tag="AC16CY_04990"
     CDS             179..913
                     /locus_tag="AC16CY_04990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3353169.1"
                     /translation="MLDSQLAELADRLRRARRVTVMTGAGVSAASGIPTFRGDGGLWR
                     RFRAEELATPEAFRSDPALVWEWYDWRRQKIAACEPNPAHHVLARWTNRSDGCTVITQ
                     NVDDLHLRAGTARLIRLHGSIWELSCWSRCGAAPWRDERAPLPVLPAVCPRCGGLARP
                     AVVWFGEALSADDVDQARRACACDVFIAAGTSAIVYPAAGLLDEARSAGAFTAEINTE
                     TTAATSGVDVSLQGAAEVILPELEGL"
     gene            complement(1053..1535)
                     /locus_tag="AC16CY_04995"
     CDS             complement(1053..1535)
                     /locus_tag="AC16CY_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3353170.1"
                     /translation="MKKVLCGVAMLGWIPTLAIVTGARWMSGDPVLAQARTPVKITRI
                     YTGSDGKTKVDEYELPLKPRDRGSEASASIGVTSLSVRRTTPEYNLDWHPAPRRQLVV
                     TLAGESEIELEGGRKIRLGAGHILLAEDTTGQGHISRAVAGKDRIALDIVLADGATLR
                     "
     gene            complement(1560..2045)
                     /locus_tag="AC16CY_05000"
     CDS             complement(1560..2045)
                     /locus_tag="AC16CY_05000"
                     /inference="COORDINATES: protein motif:HMM:NF042488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HIR3353171.1"
                     /translation="MRELLAWLEGSALGHVMRESGAWTYGLVNLAHILGVSSLFGAVL
                     VLDLRLLGAWRRVPLASITRPTVTVAATGFVVAASTGLCLITANATEYVGNPFLLVKF
                     PAIAAGLMNAAVVRTLAGWKAKDTRELTRREQRQLAAAGAVSLASWLTAIAAGRLIGY
                     W"
     gene            complement(2064..2438)
                     /locus_tag="AC16CY_05005"
     CDS             complement(2064..2438)
                     /locus_tag="AC16CY_05005"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3353172.1"
                     /translation="MRMRFAIWTTAAALVAAAVPAVAHHGWGGQGTEEFQLVGTLDSA
                     VSLAGPHATMKVRAADGQVWDITLAAAARVESAGLKEGVIPVGAQVTIQGHRNRDPKR
                     FEVKTERVIWNGRTFNVYPDRK"
     gene            complement(2455..2817)
                     /locus_tag="AC16CY_05010"
     CDS             complement(2455..2817)
                     /locus_tag="AC16CY_05010"
                     /inference="COORDINATES: protein motif:HMM:NF018508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR3353173.1"
                     /translation="MANRFSSWLSRPLALRTLFAQARLAARLLREPRVPLPVKVIPIL
                     ALLYVIWPIDLVPDLFPVIGQLDDLGIALLALEMFTKMCPAVVRSFHQEAILQRRAYS
                     PMDGVSRVRGYESAKVRR"
     gene            complement(2928..>3330)
                     /locus_tag="AC16CY_05015"
     CDS             complement(2928..>3330)
                     /locus_tag="AC16CY_05015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3353174.1"
                     /translation="FGESLGTAVAVGLAAEQPPVALILRSPFTSMADVGAYHYPIPVR
                     LLLRDRFASRDTIARVRAPVLVIAGDRDDIVPLEHSRRLYDAAPFPKELVIIKGASHN
                     DDELLAGEEMIQAIVGFLDHEPGRRGDTENR"
BASE COUNT          538 a         1121 c         1122 g          549 t
ORIGIN      
        1 ggtgctgcct gactgggagc gggcgcttgc gccatcggtg cggcgaacgc aacgacagtg
       61 gctgcgagaa cgagggtgag tttcgatgtc acgaggatca tcatgatgac aacaacgcgc
      121 gccgggcaaa agggttaaca gcgacactgc aggccataga ctgaaggacc ggaggccgat
      181 gctggactct cagctggcag aacttgccga ccgtttacgt cgcgcacgcc gcgtcaccgt
      241 gatgacgggc gccggcgtgt ccgcggcgag cgggatcccg acattccggg gcgacggcgg
      301 gctgtggcgc cgcttccgcg ccgaggagct tgccacgccc gaggcctttc ggtcagatcc
      361 ggcactggtg tgggagtggt acgactggcg gcggcagaag atcgccgcgt gcgagcccaa
      421 ccccgcgcat cacgtgctcg cacggtggac caaccgatct gacggctgca cggtcatcac
      481 gcagaacgtc gacgatcttc atctccgcgc gggaaccgcc cgactgattc gcctccacgg
      541 ttccatctgg gagctttcgt gctggtccag gtgcggcgcg gcgccctggc gcgacgaacg
      601 cgcaccgctg cccgtgctgc ctgcagtgtg tccgcgatgc ggaggactgg cgcgacccgc
      661 ggtcgtctgg tttggcgaag cactctccgc tgacgatgtg gaccaggctc gccgcgcgtg
      721 cgcctgcgac gtgttcatcg ccgcaggcac ctcggcgatc gtgtatcccg cagcggggct
      781 gctggacgag gcgcgcagcg ccggcgcctt cacggcggaa atcaataccg aaacgaccgc
      841 ggcaacgtcc ggagtggacg tgtcgctcca gggcgcggct gaggtcattc ttcccgagct
      901 cgagggcctg tagcgtcgtc cttggtactt ggtgcctggt gcttggtccg tccttggtcc
      961 tttgtccgtc ctttgtcctt ggtccttggt ccttggtcct ctgcggacca gaggaccagg
     1021 aaccacggac ggaccagggc ccagcgacgt attcagcgca gcgttgcgcc gtcggcgagc
     1081 acgatgtcga gcgcgatccg gtccttgccc gccacggctc gcgagatgtg cccctgtccc
     1141 gtggtgtcct ccgcgagcag gatgtggccg gcgccgaggc ggatcttccg gcctccttcg
     1201 agctcgatct cgctttctcc agccagcgta accacgagct gccgtcgcgg ggccgggtgc
     1261 cagtcgaggt tgtattcagg cgtcgtacgc cgcacggaca ggctcgtgac gccgatcgac
     1321 gccgacgcct cgctgccgcg gtcgcgcggc ttcagcggca gctcgtattc gtcgaccttc
     1381 gtcttgccgt cggacccggt gtagatccgc gtgatcttca ccggcgttcg cgcctgggcg
     1441 aggacgggat ctccggacat ccacctcgcc cccgtgacga tggcgagcgt gggaatccaa
     1501 ccgagcatcg caacgccgca cagcactttc ttcatggcga ggcctcccgg agggcaactc
     1561 taccagtacc cgatcagccg tccggcggcg atcgccgtca accaggacgc gagcgacacc
     1621 gcgccggctg ccgccagctg ccgttgctcg cggcgcgtca gctcacgcgt gtctttggcc
     1681 ttccagcccg cgagtgtgcg caccacggcg gcgttcatga gaccggcggc gatcgcgggg
     1741 aacttgacga ggaggaacgg gtttccgacg tactcggtgg cgttggcggt gatgaggcac
     1801 aggccggtcg aggcggcgac gacgaagccc gtggccgcga ccgtcaccgt gggtcgcgtg
     1861 atcgaggcca gcggcacgcg gcgccacgcg ccgaggagcc gcaggtcgag gacgaggacg
     1921 gcgccgaaca gcgacgagac gccgagaatg tgcgccaggt tgacgaggcc gtacgtccag
     1981 gcaccggact cgcgcatgac gtggccaagg gcagatccct cgagccacgc cagcaactca
     2041 cgcacgtcgt tgacgccgcc gcgctacttg cggtcgggat agacgttgaa ggtgcggccg
     2101 ttccagatca cgcgttccgt cttgacctcg aagcgcttcg ggtcgcggtt gcgatgaccc
     2161 tggatcgtga cctgcgcacc gactgggatc acgccttcct tcaggccggc gctctccacg
     2221 cgcgctgccg ccgcgagcgt gatgtcccac acctgcccgt cggctgcgcg caccttcatg
     2281 gtggcgtgcg gcccggccag gctcacggcg ctgtccaacg ttcccacgag ctggaattcc
     2341 tcggtcccct gaccgcccca gccatgatgc gccacggcgg gaacggccgc cgcaacgagc
     2401 gccgccgccg tcgtccagat cgcgaatcgc attctcatgg ttgacctcct caagctaccg
     2461 tcgcaccttc gcactctcgt accctcgcac cctcgacaca ccgtccatcg gcgagtaggc
     2521 ccgccgctga agaatcgcct cctgatgaaa cgatcgtacg acggcggggc acatcttcgt
     2581 gaacatctcg agcgccagca gcgcgatgcc caggtcgtcg agctgcccga tcacggggaa
     2641 gaggtctggc accagatcga tcggccagat cacgtacagc aacgccagga tcgggatcac
     2701 tttgacgggc agcggcacgc gcggctcgcg cagcagcctg gcggcaagtc tcgcctgcgc
     2761 gaacagcgtt cgcagcgcca gcgggcgcga cagccaggac gagaagcgat tcgccatatc
     2821 gagccgtgat tactacgcta ccaccgaacg cgcggatgag catctccaag actcggccat
     2881 gacaactgat tgcgaaccac ggagacacgg tggcacggag gctttgatca tcggttctcc
     2941 gtgtctccgc gcctccctgg ttcgtggtct aggaatccga cgatggcctg gatcatctcc
     3001 tcacccgcga gcagctcgtc gtcgttgtgc gaggcgcctt tgatgatgac aagctctttc
     3061 gggaatggcg cggcgtcgta cagcctccgg ctgtgctcga gtggaacgat gtcgtcgcgg
     3121 tcgcctgcga tgacgaggac gggcgcgcgg actcgggcga tcgtgtcgcg cgaggcgaac
     3181 cggtcgcgca gcagcaggcg caccggaatc gggtagtggt acgcgccgac gtcggccatc
     3241 gacgtgaacg gcgatcgcag gatcagcgca accggcggct gttccgcggc gaggccgacg
     3301 gcaacggccg tgccgagcga ctcgccgaag
//
LOCUS       DBNGSH010000219         2196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1885325, whole genome shotgun sequence.
ACCESSION   DBNGSH010000219 DBNGSH010000000
VERSION     DBNGSH010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1885325"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="AC16CY_05020"
     CDS             <1..957
                     /locus_tag="AC16CY_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353175.1"
                     /translation="RRVYHQGRYTSAGRALLAVSDVATSPRDAIQRKSAVIAYNRRHP
                     DARLDRQAGYALLPPGSLPGTDEVVKLSLHLFEEKKRKIEAAAGPDTALHAKKWAFLR
                     SVLTNQDLAEHPGLVDFALSDGLLSLVTNYLGTIPHLNRVDLLYSVNHGGDEVMSSQI
                     YHLDPEGRRQAKLFLNLRDVGPDEGPFTFIPASDSERLVKAIKRRRSTDAADTDEAGV
                     VQGRYFDRELADVGGLDRAISVMGPAGSAGLVDTSRCLHCGSRVKPGTYRLCLYIQYC
                     TSREHGNLFDISRHATDPVRYLATVNSRRSANAAVAAPHQMG"
     gene            1026..1355
                     /locus_tag="AC16CY_05025"
     CDS             1026..1355
                     /locus_tag="AC16CY_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3353176.1"
                     /translation="MSDQTDVLQGTLDLLILRTLGLEPMHGWAIAQRIQQISNDLLRV
                     QQGSLYPALHRLEHQGWITADWGVSENNRRARFYSLTRAGRKQVEAEVAKWERLSAGV
                     DLVLQRT"
     gene            1427..>2196
                     /locus_tag="AC16CY_05030"
     CDS             1427..>2196
                     /locus_tag="AC16CY_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353177.1"
                     /translation="MSEELQLHLERETERLQASGLSREDARLQARRLFGGVEQIKEAS
                     RDARGMAALDTLVRDTRYGLRRLVRDWRFTTAAVLILGLAIGANTAIFSVVNAVLFRK
                     QAFANSDRLVNIYQNDRAGQPLVVVSYDAYKRIAEYRDVFSTTMAGSIPNPGRYLHDG
                     AVRDAVVEFATATYLDVLGRRPSLGRWFDESEERAGAPLVAVVGYQAWTTVFRSDPSL
                     IGRVVRIEGAPVTIIGIGPANFRGNLDIGLGTDFWLPIT"
BASE COUNT          383 a          767 c          685 g          361 t
ORIGIN      
        1 cgacgcgtct atcatcaggg ccgctacacg agcgcggggc gtgcgttgct ggccgtctcc
       61 gacgtggcga cgtcgccgcg cgatgcgatc cagcgcaaga gcgccgtcat cgcctacaac
      121 cggcgccacc ccgacgcgag gctcgaccgc caggccggct acgcgcttct gcctcccggg
      181 tcgcttccag gcacggacga agtcgtcaag ttgtcactgc accttttcga ggagaagaaa
      241 cggaagatcg aagcggctgc cggtccagac accgcacttc acgccaagaa atgggccttc
      301 cttcgcagcg tcctgacgaa ccaggacctg gccgagcacc ccgggctcgt ggactttgcg
      361 ctgtcggacg gcctgctgag cctcgtgacg aactacctcg ggaccatccc ccatctcaac
      421 cgcgtcgatc tcctgtactc cgtgaaccac ggtggggacg aggtgatgtc gagccagatt
      481 taccatctcg atcccgaggg ccgccggcaa gcgaaactgt tcttgaacct gcgggacgtc
      541 gggccagacg aagggccgtt caccttcatt cccgcgtcgg acagcgagcg cctcgtgaag
      601 gccatcaagc gccgccgctc cactgacgca gccgacaccg acgaagccgg cgtcgtccag
      661 gggcgctact tcgatcgcga gctcgccgac gtgggcggac tcgacagggc gatcagcgtc
      721 atgggacccg ccggaagcgc cgggctcgtg gacacgtcgc ggtgcctgca ttgcgggagc
      781 cgcgtcaagc ccggcacgta tcgactctgt ctctacatcc agtactgcac ctctcgcgag
      841 cacggcaatc tgttcgacat ctcccgacac gcaaccgatc cagtgcgcta tctcgcgacc
      901 gtgaacagcc gccggtccgc caacgcggcg gtagcggcgc cccaccagat gggctgacgc
      961 gttcccggtg aacctttcac cggtctcgtc cgtcttgtcc ctatggcaat ctacaagaga
     1021 ggtacatgag cgaccagacc gacgtccttc aaggcaccct cgacctcctg atcctgcgta
     1081 ctcttgggct cgagccgatg cacgggtggg cgatcgcgca gcgaatccag cagatttcca
     1141 acgacttgct gcgggttcag cagggatcgc tctatccggc gctgcatcgc ctcgaacatc
     1201 aaggctggat tacggccgac tggggcgtgt ccgaaaacaa ccgccgggcc cgcttctatt
     1261 cgctgaccag ggccggccgc aagcaagtcg aggcagaagt cgcgaagtgg gaacgcctgt
     1321 cggcgggcgt tgacctcgtg ctgcaaagga catagccctg cgcgcctggc acatcctccg
     1381 cagccggctc cgctcgctcc tgttccgcgg cagccgcgag tccgaattga gcgaagagct
     1441 gcagctccat ctcgagcgag agaccgaacg tctgcaggcg agcgggctgt cgcgggagga
     1501 cgcgcgcctt caggcgcgtc gcctgtttgg cggcgtcgag caaatcaagg aagccagccg
     1561 cgacgcgcgc gggatggccg ccctggacac gctggtgcgc gacacacgct acggcctccg
     1621 ccggctggtg cgcgactggc gtttcaccac ggcggcggtg ctgatcctcg gcctcgctat
     1681 cggcgccaac acggccatct tcagcgtcgt gaacgcggtc ctcttccgga aacaggcgtt
     1741 cgccaactcc gaccgcctcg tcaacatcta tcagaacgac cgcgcgggcc agccgctcgt
     1801 cgtcgtctcg tacgacgcct acaaacggat agcagagtac cgagacgtgt tctcgacgac
     1861 gatggctggc agcatcccga accccggccg ctaccttcac gacggcgccg tccgcgacgc
     1921 agtcgtcgag ttcgcgacgg cgacctacct cgacgtcctg ggacgccggc cgtcgctcgg
     1981 ccgctggttc gacgagtccg aggagcgggc aggggcgccg ctcgtggccg ttgtcgggta
     2041 tcaggcgtgg accacagtct tccgctcgga cccatccctc atcgggcgcg tcgtcagaat
     2101 cgaaggcgcg ccggtgacca tcatcggcat cggtccggcc aatttccgcg gcaacctgga
     2161 catcggtctc ggcacggatt tctggctgcc gatcac
//
LOCUS       DBNGSH010000220         3152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2394692, whole genome shotgun sequence.
ACCESSION   DBNGSH010000220 DBNGSH010000000
VERSION     DBNGSH010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2394692"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3108)
                     /locus_tag="AC16CY_05035"
     CDS             complement(<1..3108)
                     /locus_tag="AC16CY_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3353178.1"
                     /translation="MITSIIELVIRFRWIVVTVAAALAALSVYAIRTASLDAIPDISD
                     PQVIVYVKWPRSPQVIETEVTEPVIRALGGSPDVRAIRGTSHMGYSFVYVILNDPARR
                     AAVRQFVADRLSAVRAQLPSDAAVSIGPNASSMGWIFQYALVDKQHAHDLRELRLLNE
                     SVVERALQAAPGVAEVASVGGLEKQYQVKLFPPLLKERGLSLTTVLGAVQGAFQEAGG
                     RTIEVTNREYQLRGGVGSESIDTLEFLVLGRDRAGQPVQLKDVGFVQVGYDLRRGIAD
                     LDGEGEVVGGIAIMEQGHNVLAVTRTLLQRLEALKPSLPDGVEVVPTYNRSALIWDTL
                     TNFFRAIAYELVVVILVIAVALRNLRAAAAPVCVLLLGTLFTALPLTAFDQTINLFSL
                     AGLAIAIGEMADATIVIVENCTAQLARRGALDAREKLRVIVHATATMTRPLLFSMLII
                     VTSFLPIFFLGEREGRLFNPLAFTKTFAMAFSTLLTLVLLPVVIVWVFKARVPAPSDG
                     RESAFVRAYRAMLGRTIRNRYAFVGGSMVLLVLAIVLMARFQKDYMPEMEEGSILYMP
                     TTLPGLPSREAGWIVQQMDRKLKSFPEVERVFGKLGRADTATDPAPVEMIETTVTLKP
                     RSEWRPGMTKDKLIAEMNDAMQIVGYANSWSQPIGTRVVMQSTGIQTPVGIKVKGKDL
                     ATVQEIAQEVERLLRDLPATQSVFAERISQGYFVDARLDLVRMAAQGVTVDEALPTVR
                     FAIGGDNLVSIRQPDQTVVPLAIQYSPEYIDTLEKVRNTPVITQDGRSVALSEIADVG
                     VREAPEMIRNDNGEPAGYVFVYPRGVTGPEYVERARQHLQANLPMPAGYSTEWTGSYQ
                     FAEEARSKLQVVVPVTLVIMFALLLMAFRSVADSSLIMLSAPFALVGGVLLQWQWGFT
                     MTTAVIIGYVSLFAVAIQTGIIMIEFIREALANRPAGQSYVDAVIEGSVARLRPKLMT
                     VATTVLGLLPIMFASGAGMDITQPIATPTFGGMISSTIYVLFLIPCLFAIGDDIRRRW
                     PERFGA"
BASE COUNT          523 a         1086 c         1035 g          508 t
ORIGIN      
        1 agccccgaag cgctcgggcc agcggcggcg gatgtcgtcg ccgatcgcga acaggcacgg
       61 gatcaggaac agcacgtaga tcgtcgagct gatcattccg ccgaacgtgg gcgtggcaat
      121 cggctgggtg atgtccatgc cggcgccgct cgcgaacatg atcggcagca ggcccagcac
      181 cgtcgtggcc acggtcatca gcttgggcct cagccgcgcg accgaaccct cgatgacggc
      241 gtccacgtac gactgcccgg cgggccggtt cgccagcgcc tcgcggatga actcgatcat
      301 gatgatgccc gtctggatcg cgacggcgaa cagcgagacg tagccgatga tcaccgccgt
      361 ggtcatcgtg aacccccact gccactgcag gagcacgccg ccgaccagcg caaacggggc
      421 cgacagcatg atgagcgagc tgtcggcgac cgatcggaac gccatgagca gcaacgcgaa
      481 catgatgacg agcgtcaccg gaacgacgac ctgcagcttc gatcgggcct cctcggcgaa
      541 ctggtacgac ccggtccact ccgtcgaata cccggcgggc atcggcaggt tcgcctgcag
      601 gtgctgccgc gcgcgctcca cgtattccgg ccccgtgacg ccccgcggat acacgaagac
      661 gtagccggcc ggctcgccgt tgtcgttgcg gatcatttcc ggcgcttcgc gcacgccgac
      721 gtcggcaatc tcgctgagcg ccaccgagcg cccatcctgc gtgatgaccg gcgtgttccg
      781 gactttctcc agcgtgtcga tgtattccgg ggagtactga atggcgagcg gcaccaccgt
      841 ctgatcgggc tgcctgatgc tgacgaggtt gtcgccgccg atcgcgaacc gcaccgtcgg
      901 cagcgcttcg tccacggtca cgccctgcgc ggccatcctc acgagatcga ggcgcgcgtc
      961 cacgaagtag ccctgcgaga tccgctcggc gaagaccgac tgggtggccg ggagatcgcg
     1021 caggagccgc tcgacctcct gtgcgatctc ctggacggtc gccaggtcct ttcccttgac
     1081 cttgatgccg accggcgtct ggatccccgt gctctgcatg acgacgcgcg tgccgatcgg
     1141 ctgactccag ctgttggcgt agccgacgat ctgcatcgcg tcgttcatct cggcgatcag
     1201 cttgtccttg gtcatccccg gccgccactc cgacctcggc ttcagcgtga ccgtcgtctc
     1261 gatcatctcg accggcgccg ggtcggtcgc cgtatcggcg cggcccaact tgccgaacac
     1321 gcgctcgacc tccgggaacg acttcagctt ccggtccatc tgctggacga tccagccggc
     1381 ctcgcgcgat ggcagtcccg gcagcgttgt cggcatgtac aggatcgagc cctcctccat
     1441 ttcgggcatg tagtccttct ggaagcgggc catcaggacg atcgccagaa cgagcagcac
     1501 catgctgccc ccgacaaacg cgtagcggtt gcgaatcgtc cgcccgagca tcgcgcggta
     1561 cgcgcggacg aacgcgctct cgcgcccgtc ggacggcgcc ggcacccgcg ccttgaacac
     1621 ccacacgatc acgacgggca gcaggacgag cgtcagcagc gtcgagaacg ccatcgcgaa
     1681 cgtcttggtg aacgccaacg ggttgaagag gcgcccctcc cgctcgccga gaaagaagat
     1741 cggcaggaac gacgtcacga tgatgagcat cgagaagagc aggggccgcg tcatcgtcgc
     1801 cgtggcgtgg acgatcaccc gcagcttctc tcgcgcgtcg agcgccccgc gcctggcgag
     1861 ctgcgccgtg cagttctcga cgatgacgat ggtcgcgtcg gccatctcgc cgatggcgat
     1921 ggccagtccc gccagcgaga agagattgat ggtctggtcg aacgccgtga gcggcagagc
     1981 ggtgaacagc gtgccgagga gcagcacgca caccggagcg gcggccgcgc gcaggttccg
     2041 cagcgccacg gcgatcacga ggatcaccac gacgagctcg tacgcgatcg cccggaagaa
     2101 gttcgtcagc gtgtcccaga tcagggccga ccggttgtag gtcgggacga cctcgacgcc
     2161 gtcggggagc gacggtttca gtgcctccag cctctgcagg agcgtccgcg tgaccgcgag
     2221 gacgttgtgc ccctgttcca tgatcgcgat gccgccgacg acttctccct cgccgtcgag
     2281 gtcggcgatt ccgcgcctga ggtcgtagcc gacctggacg aatccgacgt ccttgagctg
     2341 gacgggctgc cccgcgcggt cgcggccgag cacgaggaac tcgagcgtgt cgatgctctc
     2401 gctgccgacg ccgccccgga gctgatactc gcggttggtg acctcgatcg tgcggccgcc
     2461 cgcttcctgg aacgcgccct ggacggcgcc gaggacagtc gtgagagaca ggccccgctc
     2521 cttcaggagc ggcggaaaca gtttcacctg gtactgcttc tccagcccgc cgaccgaggc
     2581 gacctcggcc acgccgggcg cggcctgcag cgcccgctcg acgacgcttt cgttcagcag
     2641 ccgcagctcg cgcaggtcgt gcgcgtgctg cttgtctacc agtgcgtact ggaagatcca
     2701 ccccatgctg ctcgcgttgg ggccgatgct gaccgcggca tcggacggca gctgggcccg
     2761 cacggcgctg aggcgatcgg cgacgaactg ccgcacggcc gcgcgtcggg ccgggtcgtt
     2821 caggatgacg tagacgaacg agtaccccat gtgcgaggta ccccggatcg cgcgcacgtc
     2881 gggcgatccg ccgagcgcac ggatcaccgg ctcggtgacc tcggtctcga tcacctgcgg
     2941 gcttcgcggc cacttcacgt agacgatgac ctggggatcg gagatgtcgg gaatcgcatc
     3001 gagcgacgcg gtccggatcg cgtagacgct gagcgccgcg agcgccgcgg ccactgtcac
     3061 cacgatccag cggaagcgga tgacgagctc gatgatcgac gtgatcatgt cgttcgcact
     3121 tctctcttct cgcttctcag ttcgtgttca gt
//
LOCUS       DBNGSH010000221         4700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3074376, whole genome shotgun sequence.
ACCESSION   DBNGSH010000221 DBNGSH010000000
VERSION     DBNGSH010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3074376"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="AC16CY_05040"
     CDS             <1..1049
                     /locus_tag="AC16CY_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR3353179.1"
                     /translation="LSTAYEGAIMNALIRCGVALVLVFTSTAAFSQVGIVQTNSQGDN
                     IHLIDPATQRIVGEVRGIPINHGAAASPDGRQLYFSSEAEQTLHVVDARTLQTTKKIP
                     LTGRPNNISISRDGRRVYVGIVSQPGAVDVIDTASLDKVKSIPTKGGIHNVYVTPDGR
                     HVVAGSIAGRLMTVIDQKTEEPIWTLFEEGVRPMAFETNADGSTKRIYVQLSNLHGFA
                     VVDFQQRKEVARVELPNDVPAEKIDKGPFNASPSHGLGVAPDGKSLWVTSRPNARVYT
                     YTLPDLKLLPDPVDLGGRPDWVTFTPDSKLIYITTENTDLVHAIDVTTRKEVAQIKVG
                     RAPKRNITARLAGS"
     gene            complement(1165..1815)
                     /locus_tag="AC16CY_05045"
     CDS             complement(1165..1815)
                     /locus_tag="AC16CY_05045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3353180.1"
                     /translation="MTRTSAAVLIALAVVVSAVVGVAAQSAAALKDPAKLKEQAPAKY
                     NARFDTSVGTFVVTVTREWAPLGADRFYNLVKNGYYDNVRFFRVIPNFMVQFGINGDP
                     ALNAVWRNARIGVDPVKQRNMRGYITYAMGGSPDTRTTQVFINFRDNSSLDAQGFAPF
                     GQVTTGMDVVDKIYQGYGEGAPRGDGPDQGRIQAEGNAYLMKSFAKLDYVKKATIE"
     gene            complement(1938..3200)
                     /locus_tag="AC16CY_05050"
     CDS             complement(1938..3200)
                     /locus_tag="AC16CY_05050"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3353181.1"
                     /translation="MPIKRLGVVVGGGPAPGTNGVIAAVAMEAIKGGCTPIGFHDGFD
                     WLAQRYTDEQHELTIDEVSRIHLDGGVLLGSSRRDVTRDAESLENTVAALTKLRIDAL
                     VCIGGDDMVRSAVTIERRGVVNVVQVPKSIDNDLWLPSPLATLGYETARHVGVGIVKS
                     LMEDARTTGRWYLGVTMGRPTGHLTLGIGKAASATCTIIPEEFPETPISLSGIADIVE
                     GSIIKRRAMGKSYGVVLLSEALVEGFSPREVAELQDVDRDAQGNIRVTEIDLGRKVKN
                     EVQMRLEQRGIKVTMVDKTIGYELRCAPPIPFDAEYARDLGYAAVTYLLQGGSGSLVT
                     IQGGEFTPVPFEHVVDPSGAGRRRAVDITTENYRVAREYMVRIGPRDFRDPAWVAALA
                     RAGALDEDAFRMRFGRFASSVPEQITGR"
     gene            complement(3214..4470)
                     /gene="pfp"
                     /locus_tag="AC16CY_05055"
     CDS             complement(3214..4470)
                     /gene="pfp"
                     /locus_tag="AC16CY_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HIR3353182.1"
                     /translation="MGPPGRLGILVGGGPAPGINAVISAATIEALNQEFGVIGIHEGF
                     KRLVRRDASALRPLAFDDVSRVHLLGGSILGTSRENPTSSPEATLAVIETLREAGITH
                     LITIGGDDTALSSRHISEQSGGQIRNVHVPKTIDNDLPLPPHVSTFGFQTARHVGVEL
                     VRNLMEDAKSTRRWYVVVAMGRKAGHLALGIGKAAGATLTVVAEEFAAAHVPFATICD
                     IVEGAIIKRRAMGRHFGVAVLAEGLIDRLDPSEVVELQDVERDEHGHVRFAEVDLARK
                     VKAEVQGRLSGRGLRVTITNKNVGYELRCADPISFDAEYCRDLGYGAVRFLLDGGSGA
                     MVSVQNGRFVPIPFDDLVETGSGKAKVRLVDVDSEGFRVARAYMIRLDAEDFVHAAWV
                     DRLADAAHLTPGAFRERFEYLASTRP"
BASE COUNT          811 a         1554 c         1521 g          814 t
ORIGIN      
        1 tactctccac ggcgtacgaa ggagccatca tgaacgcact catccgctgc ggcgtggcgc
       61 tggtcctggt gttcacttcg accgccgcct tctcacaagt cgggatcgtc cagaccaact
      121 cgcaggggga caacatccac ctgatcgatc ctgcgacgca acggatcgtc ggcgaagtca
      181 gaggcattcc gatcaaccac ggcgccgcgg cgtcgcccga cggcaggcag ctctacttca
      241 gcagcgaggc cgagcagacg ctccacgtcg tcgacgcgag aaccctgcag acgacgaaga
      301 agatcccgtt gacgggccgc ccgaacaaca tctcgatcag ccgcgacgga cggcgcgtgt
      361 atgtcggcat cgtctcccag cccggcgccg tcgatgtcat cgacacggca tcgctcgaca
      421 aggtcaagag catcccgacc aagggcggca tccacaacgt ctacgtgacg ccggacggca
      481 ggcacgtcgt ggccggctcg atcgcgggca ggctcatgac cgtgatcgac cagaagaccg
      541 aggaaccgat ctggacgctg ttcgaggagg gcgtgcgccc gatggcgttc gagacgaacg
      601 ccgacggctc gacgaagcgg atctacgtgc agctgtcgaa cctccacggc tttgcggtcg
      661 tcgattttca gcagcggaag gaagttgcgc gcgtcgagct gccgaacgac gtcccggcgg
      721 agaagatcga caagggcccc ttcaacgcgt ctccctcgca cgggcttgga gtcgcgccgg
      781 acgggaagtc gctgtgggtg acgagcaggc cgaacgctcg cgtctacacc tacacgcttc
      841 cggatctgaa gctgctgccg gacccggtcg atctcggcgg acggcccgac tgggtgacct
      901 tcacgccgga cagtaagctg atctacatca cgacggagaa caccgacctc gtgcacgcga
      961 tcgacgtcac gacgcggaag gaggtcgcgc agatcaaggt gggaagggcc ccgaagcgga
     1021 acatcaccgc gaggctggct ggcagctaac tggcgaaccc ggaagtcgcc gtattcacgc
     1081 cgagcacgac acactgagtc accgagacac cgagaaacgc ttggcgaatc tgcctcggtg
     1141 cctcagtgac tcggtgtgtc gcgatcactc gatggtcgcc ttcttgacgt aatcgagttt
     1201 cgcaaagctc ttcatcaggt acgcgttgcc ctcggcctga atgcgcccct ggtcggggcc
     1261 gtcgccgcgc ggagcgccct cgccgtagcc ctgatagatc ttgtccacga cgtccatgcc
     1321 ggtggtcacc tggccgaacg gggcaaagcc ctgcgcgtcg aggctgctgt tgtcgcggaa
     1381 gttgatgaac acctgcgtgg tccgcgtgtc gggcgagccg cccatcgcat aggtgatgta
     1441 gccgcgcatg ttcctctgct tcaccgggtc cacgccgatg cgcgcgttgc gccacaccgc
     1501 gttcagcgcg gggtcgccgt tgataccgaa ctgcaccatg aagttcggga tgacccggaa
     1561 gaaccgcaca ttgtcgtagt agccgttctt gacgaggttg tagaaccgat ccgcgccgag
     1621 aggtgcccac tctcgcgtga cggtgacgac gaacgtcccg acgctggtgt cgaagcgggc
     1681 gttgtacttc gccggggcct gttccttgag cttggccggg tctttcaatg cggcggcgga
     1741 ctgggcggcc actcccacga cggccgatac gacgacagcc agagcgatca ggacagcggc
     1801 ggacgtgcgg gtcatggagt ctcctccgtg aaagaagcca cgaaggacgc gggccgttcg
     1861 tggtctcgac gattgcgaac gctacacact ctaccgcgaa cggggtcgtt tgtcccggga
     1921 ttcgcccggt ggtcgcgcta tcgtcccgtg atctgctcgg gcacgctgct cgcgaaccgg
     1981 ccaaacctca tgcggaacgc gtcctcgtcg agtgcgccag cgcgcgccag cgcggccacc
     2041 caggcggggt ccctgaagtc gcgggggccg atccgcacca tatactcgcg cgcgacgcga
     2101 tagttctcgg tcgtgatgtc caccgcgcgt cgccttccgg ctccgctggg gtcgacgacg
     2161 tgctcgaaag gaaccggcgt gaactccccg ccctggatcg tcacgagcga acccgatccg
     2221 ccctgaagga ggtaggtgac ggcggcgtac ccgaggtcgc gcgcgtactc ggcgtcgaac
     2281 gggatgggcg gagcgcatcg gagctcgtag ccgatcgtct tgtcgaccat cgtgactttg
     2341 ataccgcgtt gctcgaggcg catctggact tcgttcttga ccttccgtcc cagatcgatc
     2401 tccgtgactc gaatgtttcc ctgcgcatcg cggtccacat cctgcagctc ggcgacttcg
     2461 cgcgggctga agccctcgac cagcgcttca gagagcaaca ccacgccgta cgactttccc
     2521 atcgcccgcc gcttgatgat gctgccttcg acgatgtccg cgataccgga tagcgagatc
     2581 ggtgtttccg ggaactcttc tggaatgatc gtacaggtcg cgcttgcggc tttgccgatg
     2641 ccaagcgtca agtgcccggt gggccggccc atcgtgacgc ccaggtacca gcggccggtg
     2701 gtcctggcgt cctccatgag actcttcacg atgccgacgc cgacatggcg agcggtttca
     2761 tagccgagtg ttgccagcgg cgaggggagc cacagatcgt tgtcgatgct cttgggcacc
     2821 tgcacgacgt tcacgacgcc gcggcgctcg atggtcacgg cactgcgcac catgtcgtct
     2881 ccgccgatgc agacgagcgc gtcaatccgg agcttggtga gcgcggctac ggtgttctcc
     2941 aggctctccg cgtcgcgcgt cacgtcgcgc cgtgacgagc cgaggagcac gcctccatcg
     3001 agatggatgc gcgagacttc gtctatggtc aactcgtgct gttcgtcggt gtagcgttgc
     3061 gcgagccagt cgaacccgtc atggaatccg atcggcgtgc acccaccctt gatcgcttcc
     3121 atggccacgg ccgcgatgac gccgttcgtg cccggtgccg gtcctccacc cacgaccacg
     3181 cctaggcgct tgatcggcat tcccgcgggt cccttacggc ctggtcgagg ccaggtactc
     3241 gaagcgctcg cggaaggcgc caggcgtcag atgtgcggcg tcggcaagcc ggtcgaccca
     3301 ggcagcgtgc acgaagtcct cggcgtcgag ccggatcatg taggcgcggg ccacccgaaa
     3361 tccctcgctg tcaacgtcca cgagccgtac cttcgccttc ccgcttcccg tctcgacgag
     3421 atcatcgaac ggaatcggca cgaagcggcc gttctggacg ctgaccatgg cgccgctgcc
     3481 cccgtccagc agaaagcgca ccgcgccgta accgagatcg cggcaatact cggcatcgaa
     3541 agaaatcgga tcggcgcatc tgagctcgta gccgacgttc ttgttcgtga tcgtgactcg
     3601 gagcccgcgt ccgctgaggc gtccttgcac ctccgccttg accttccgag ccaggtcgac
     3661 ttccgcgaat cgcacgtgcc cgtgctcgtc gcgctcgacg tcctgcagct cgacgacctc
     3721 ggagggatcg agcctgtcga tgagcccctc ggcgaggacc gcgacgccga agtgacgccc
     3781 catcgcgcga cgcttgatga tcgccccttc gacgatgtcg cagatcgtcg caaacggcac
     3841 gtgcgcggcg gcgaactctt cggcgacgac cgtgagcgtg gctcccgccg ccttgccgat
     3901 gccgagcgcg aggtgcccgg ccttgcgccc catcgccacg acgacgtacc agcgtcgagt
     3961 ggacttggcg tcctccatca ggtttcgtac cagctccacc ccgacgtggc gggcggtctg
     4021 gaatccgaac gtggagacgt gcggcggcag cggcagatcg ttgtcgatcg tcttcggcac
     4081 gtgcacgttc cggatctggc cacccgactg ctcgctgatg tggcgcgacg agagtgccgt
     4141 gtcatcgccg ccgatcgtaa tgagatgcgt gatgcccgcc tcgcgcagcg tctcgatgac
     4201 tgccagcgta gcttcgggag acgaggtcgg attctcgcga gaggtcccca ggatcgagcc
     4261 tccgagcagg tgcacccgcg acacgtcgtc aaaggcgagc ggccgcagcg ccgatgcatc
     4321 gcggcggacg agacgtttga acccctcgtg aatgccgatg acgccgaatt cctggttgag
     4381 ggcttcgatg gtcgcggcgg agatgacggc gttgatgccg ggcgccggac cgccgccgac
     4441 gaggatgccg agtcgccccg gtgggcccat gagtcgggat tttcgcggcc ccgtgccgta
     4501 gtgtcaaacg accgtccggg tgccgggaag agtcaacagc gatggcacga cgtggcactc
     4561 gtcagagccg tggcaaagac cctgtgtgtg caggtgtgcg aacctgctca ttgcgcccct
     4621 tgactcgccc ggcggaggcg tccaagatcg cgacggcagg acgtgtcgcg acctgaggga
     4681 gctgatggca cctgacgctg
//
LOCUS       DBNGSH010000222        16348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2055567, whole genome shotgun sequence.
ACCESSION   DBNGSH010000222 DBNGSH010000000
VERSION     DBNGSH010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 16348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16348
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2055567"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="AC16CY_05060"
     CDS             complement(<1..1664)
                     /locus_tag="AC16CY_05060"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3353183.1"
                     /translation="MFEPGSQLGRYEIQRRLGRGGMGAVYVAHDPVLGRMVAIKVFSG
                     DLDVPDARERFSREARAAAALNHPNIVTVFDFGSYDSQPYIVMEYVPGETVAGLIRRK
                     VPVTMADKLRWMEELAAGAGYAHQMQLVHRDIKPANLMIDRNGRLKILDFGIARILGA
                     ASNTSVMIGTPGYMAPEQITGDPVDHRADQFAMGVVFYELLAYSEAFPGDTLPMITHK
                     ILTEEPVPLERLAPDVSPDIIAIVVKALRKTAAERYDDSEALRAAISRVRKEFSSNAA
                     WNAPTILASRDTPPAGMGTRGTGSARRRQDDAVGVAQMTPPPDPKRTDREAIARRRLA
                     QIEVALQQARELLAEGHLEAALDACQQALTFDETHAGAIDLEAEINVAIELREHAARA
                     SAATAATPFDRSPSTDDMPPADAVTRLDDQFPSRASGVSDRTVLRPPGVAAPRRTPAP
                     SPPPAVPPPQPVAETPVARAPVAKVPAAKAPAPAVRKTTTPRPDPSKQIAALKARLLS
                     GFSPVAKAMRDKRTVVWAGAGAAIVLVAAVVGGLMMFGSPQTGSVV"
     gene            complement(1817..3136)
                     /locus_tag="AC16CY_05065"
     CDS             complement(1817..3136)
                     /locus_tag="AC16CY_05065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3353184.1"
                     /translation="MTHRRLGSSLLVCVVCVTLACSFAWLPLHAGSITTAKPEDVGLS
                     SERLARIHEAVQRHIDAGSLAGAVTLVSRNGRIAHLEAHGLIDVEAKRPMPRDGVFRL
                     ASMSKPVTAVAVMMMLEEGKLRLNDPVSRFIPEFKSAKVAVARPGARGGAAPPAGPQA
                     GRGGPPPEVDLVSATREVTIRDLLTHGSGLMSGGLGNAVAGQAAARTPSDTLATYIPK
                     LGAVPLDFQPGTLWRYSGLAGFDVLSRVVEVASGKTFDQFLRERLFDPLGMKDTGFTL
                     TAAIQPRLVPLYRRGPNGLERSPDQSGLSSATYFSGAGGLVSTAEDYAQLATMLVMGG
                     ELNGKRYLSPRTIELMASNHTGDLAGGQMGMSPRGIGFGLGVQVVEDPVAADRRVTKG
                     AWGWAGAYGTNVHIEPAAGMVTIILMQTSTPALQRDFENAVAQAVIK"
     gene            complement(3462..4160)
                     /locus_tag="AC16CY_05070"
     CDS             complement(3462..4160)
                     /locus_tag="AC16CY_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3353185.1"
                     /translation="MALAAGGQGERQLPPLAIESLGPTESFAFYCAPCHGRGGRGDGP
                     VAAALRARPADLTTLAERNGGEYPRDRVRAYIAGVGRPVAAHGASDMPVWGPAFRALD
                     ASDTRAHVRIDGIVDHIESLQRVENGATLFRQYCASCHGSAGRGDGPMASALRKAPAD
                     LTQFAKRNGDVFPGERVRRIIDGRDVSSHGDRTMPVWGDAFRRATPEGDETVSARIAA
                     LVRYLQSIQERAAE"
     gene            4364..4963
                     /locus_tag="AC16CY_05075"
     CDS             4364..4963
                     /locus_tag="AC16CY_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3353186.1"
                     /translation="MARESFHLDPIGARATARHYIRDIVYGANDGLITTFAVVSGVAG
                     GALSQVAILVIGAANLAADGVSMGVGNLLAIRADEGARAAEGLPELEAYPWKHGLATL
                     IAFAAAGAIPLVPYVLPLPTPVRLPWSIGMTFTSLFLLGAARALITRDRWWRTGLETL
                     LLGLVVAIAAYGAGRLVASVVRTSELGPWSLVLGSWSVP"
     gene            complement(5127..6869)
                     /locus_tag="AC16CY_05080"
     CDS             complement(5127..6869)
                     /locus_tag="AC16CY_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HIR3353187.1"
                     /translation="MKLTVLALDYDGTIARDGEVVPSVRESIAALRSSGTTVLLVTGR
                     ILDELRGVAGDLRFVDGVVAENGAVVHLPESDHTTVLAPRIPEVFLAEITRRGIPFQS
                     GHCLVDADADESQRLLEAIRALELPLVAAFNQSRVMIVTQGVSKATGLHAALDMLRLS
                     ARNTLAIGDAENDHELLRLAEVGAAVEWGSGALKSAADLVVAGTGPEAIAAFLSPLAA
                     TRTLPRPTRSRRYLRLGHSEDGREIALTVRERDVLIAGDAKSGKSWMAGLLCEQLILL
                     GYCVCVIDPEGDYRSLEALPGVTVLGGEEPPPTPRQLLRALRYPDRSAIIDLSHLRQD
                     QKIEYARAVLPSLSEIRRSTGLPHRIVIDEAHYFLHDADAGRLLDFDMNGYVVVTHLA
                     SRLPARLLEATEVIIVTCESNPVEVDALWQRSAVCAQGDIATWRQTLGHLPIGRAVIV
                     PLAEQAAGVLCPFVVGRRLTTHVRHREKYVDVPVTDSRAFQFADYGGEPPRTARTLRE
                     FVAEIERSPVEALRAYLRRGDFSRWIGEVFGDHALASELLDLEARHRYDGERDVVADV
                     IAAVRRRYDLSLGD"
     gene            6921..8480
                     /locus_tag="AC16CY_05085"
     CDS             6921..8480
                     /locus_tag="AC16CY_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside
                     phosphotransferase/ATP-binding protein"
                     /protein_id="HIR3353188.1"
                     /translation="MAQVLFDTQQDVIDCLMDPATHGGSAVSRVETHSAIVFLAGARA
                     LKLKRAVRFDYLDFSTESLRKAACEAEVRINRLAAPSLYRGVIPVTRDASGSLALGGR
                     GVPVDWLVDMVRFDEEGLFDRLAARGALNIDLMPPLAAAIARFHEAADRRDDHGGRAG
                     MQWVVDGNAAGFAEQGAGVLYPALCSELTGKAKAEIDRAGDLLDRRVREGRVRQCHGD
                     LHLRNIVLLEGKPTLFDAVEFNDEIACVDVLYDVAFLLMDLWRRRLEPHANAVFNRYI
                     AESGDRSGLALLPLFLSCRAAIRAKTSATAARMQHDGARAGELRDLAREYLVMARDFL
                     EPGPPRLIAVGGFSGSGKSTLARAIAPSIGRAPGAVVVRSDEVRKSLLGMPIQGRLGP
                     EGYTADVTDRVYRMVSEQVLEALEAGHSAVADAVFARSSDRLAIEQLATKAAVPFLGL
                     WLDAPADVLSGRTETRGPDASDAGPSIVRRQVAEGHGEISWHVLDASASAHVVAEQAG
                     ALAGAAPAAKQ"
     gene            complement(8548..8624)
                     /locus_tag="AC16CY_05090"
     tRNA            complement(8548..8624)
                     /locus_tag="AC16CY_05090"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8588..8590),aa:Pro,seq:ggg)
     gene            complement(8635..9168)
                     /locus_tag="AC16CY_05095"
     CDS             complement(8635..9168)
                     /locus_tag="AC16CY_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3353189.1"
                     /translation="MTSKYFKAVDVCEMAQLQPYVLRSWEKEFPGIGVQKSPETPRLY
                     RQSDLEQVQRIKQLVFGEGLTLAGARKRLEQDAPAQQGASEEEVAEVLEALASDARTR
                     IAHVRDGLKSILKMLSAAPGTVVVVDDYRLESPVAAKRGVRRSLAARPKARVVSKSVS
                     KRTARKSTSGSKRKRLA"
     gene            complement(9281..10732)
                     /gene="der"
                     /locus_tag="AC16CY_05100"
     CDS             complement(9281..10732)
                     /gene="der"
                     /locus_tag="AC16CY_05100"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3353190.1"
                     /translation="MPRSLASVVLIGRPNVGKSTLFNRVTKTRRAIVTTVPGTTRDVI
                     AQPVEWRGARFELTDTGGMFGASEDPLHALVLERGRRAIRDADLLVLVVDGREGLVAG
                     DRDISKVVHDAGKPALVAVNKMDDRRAASGVIDMYQLGFDPVLEVSAEHGDGVGDLLD
                     AIVEALPHTSAPSAAGPVADEVAVAIVGRPNAGKSSLVNRLLREERMIVNEAPGTTRD
                     AVDTVLAWHRRSFRIVDTAGIRRPGRVARGGQVETVSVLLARRAIETADVVVVVVDAS
                     VGATDQDAAIAGEADRLGRGVIVAANKWDLLKGRGPDFVKEFDEKLREQLKFLDYATV
                     LHISAATGERTPRLLETIDKVARSRRQRVKTADLNRLVERIAATHPPASPGKRHVRIL
                     YAAQTGVAPPTFVFFTNVATSFHFSYQRYLQNCLREEFGFEGTPLRIHVRARQRTGSR
                     GSKSARGSSGRRSNGSTGSKGSPGSRTPRRGRQ"
     gene            10638..11078
                     /locus_tag="AC16CY_05105"
     CDS             10638..11078
                     /locus_tag="AC16CY_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3353191.1"
                     /translation="MARRVFVTRLNKVDFPTFGRPISTTEARDLGTSLTVVDRLSDYF
                     YSGYRVFWTRRPRMIKVDIVNEVSKIADITKVKAEVAVDAVFDAMRLSMQRGDRIELR
                     GFGVFQVKPRKRGIGRNPRTGKEVRIPPGRTIRFKPGKDLQNIG"
     gene            11088..12080
                     /locus_tag="AC16CY_05110"
     CDS             11088..12080
                     /locus_tag="AC16CY_05110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3353192.1"
                     /translation="MGSPFPSKPPDVRLRAEPPSAQEPVYVMPAAPKPERVWRYVVLF
                     GLTLVTTTLVGMGHYESFYKGFQSVELPFSDWELATRGLWYSTSILAILGAHEFGHYY
                     ACRYYGVSASLPYFLPVPFALTGTLGAFIRIRQPIPSKRELFDIGIAGPIAGFLVAVP
                     VLMIGMYLSTVVQLPQDFDGLVFELGEPLLFQASAWLTFGTVAEGYTVNMHPMAFAAW
                     FGLLATALNLFPMGQLDGGHIAYAVLGRRSTLVTAGTVLSLVGLSFVSSSWIVWTVLT
                     ISMLVVFGPRHPRTFDEHVPLDRARMWLALFALAMFVVCFSPAPIEVIDLVRGR"
     gene            complement(12087..14297)
                     /gene="priA"
                     /locus_tag="AC16CY_05115"
     CDS             complement(12087..14297)
                     /gene="priA"
                     /locus_tag="AC16CY_05115"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR3353193.1"
                     /translation="MRQVSVAVPVPFLDLLSYNVPNHIVLPPIGGRVRVPVGSRTLTG
                     CVVGHGSSSEAPTNALKDVVETIDFEPLLPPHIVELCRWVADYYIAGVGDAIASAMPP
                     GARRKPTSFRTHRVAAVTAHGLSPDVGRLTERQRQVIEVLAAAPVGLPLSELRDRGVT
                     ADVVGRLAARGLVTIRTEDTDRDPFGRAAMPAAWASRDLTAEQATALETLAALADAQE
                     FRVALLHGVTGSGKTEIYLRLADRARRSGRQTLLLVPEIALTPSAAGLFRAALGDRVA
                     IQHSGLADGERHDQWHRIRRGDVDVVVGTRSAVFAPLTRLGIIVVDEEHDGSYKQEET
                     PRYHGRDVAILRGSRENALVVLGSATPSMESYQHAVSGKYTLATLERRVLDRPLASVR
                     LVNMREEYAEQGPDVVISRELASAIEARLLRSEQVLVLLNRRGYSTAVFCRQCGDAFD
                     CPNCTISLTVHRARQGWRARCHYCNYSTMVPKACRKCAAPYLEQTGFGTEKVEQHLRD
                     RFPAARIGRVDRDSVRRKGALASILARFASGDLDVLVGTQMIAKGHDFPRVTLVGVIS
                     ADVGLGVADFRAGERTFQLLTQVAGRAGRGEWAGEAVVQTLYPEHYSIQLACRQDFAA
                     FFERELAYRRSMRYPPAVALVNVVARGRSFDDAMTTANDIVGRLRKAGEASSFTILGP
                     APAPLVRLRGEHRVQFFLKGARRSDMRTALKAVLAELPDARRKVTIDVDPLSVL"
     gene            complement(14305..14874)
                     /locus_tag="AC16CY_05120"
     CDS             complement(14305..14874)
                     /locus_tag="AC16CY_05120"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3353194.1"
                     /translation="MFSSQADALAALRIEIGPQCTRCKLHTLGRRQVVFGVGNPNADL
                     MFVGEAPGGDEDIQGEPFVGRAGQLLTKIIEAIGMTRGDVYIANVIKCRPPNNRNPEP
                     DEVERCEPFLFRQIDVLKPKVIVALGKFAAQSLLRTADPITRSRGREYKYRSAILLPT
                     YHPAYLLRNPSAKREVWEDMKRVRAILES"
     gene            complement(15060..16286)
                     /gene="coaBC"
                     /locus_tag="AC16CY_05125"
     CDS             complement(15060..16286)
                     /gene="coaBC"
                     /locus_tag="AC16CY_05125"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR3353195.1"
                     /translation="MALIALGVTGGIGAYKAVEVCRGLQKRGHDVVAVMTRSASRFVG
                     PVTFEAITRRPVITSQWRPGMNADIEHVAIADGINLLLVAPCTANVIGKFAGGIADDF
                     LTSLYLATRAPVLLAPAMNTNMLAHEAVQRNLQTLASRGVRFVEPGEGYLACGWIGKG
                     RLAEPDEIVDAACRMLAPPDSLLRGRHVLVTAGPTYEDIDPVRYIGNRSSGRMGFALA
                     AEARGRGARVTLVAGPTRLDPPAVDEVVRVRSAAEMHAAVTAAASHADVVIMAAAVAD
                     YAPSAAAPGKLTKAEAPLTLTLTRTKDILAELGAARARSGLTTPVLVGFAAETDDLLA
                     KAREKRARKLVDLVVANDVSRPDQGFDTDTNAVTLVGEEGEESVPLQSKDRIAAAILD
                     RVERVLRVRASAATSR"
BASE COUNT         2564 a         5606 c         5437 g         2741 t
ORIGIN      
        1 atcacgacag aaccggtctg gggcgacccg aacatcatca ggcccccgac caccgccgcg
       61 acgaggacaa tcgcggcgcc ggctcccgcc cagacgactg tgcgcttgtc ccgcatcgcc
      121 ttcgccacag gggagaaccc ggacaaaagg cgtgccttca gcgccgcgat ctgtttcgaa
      181 ggatccggac gaggagtcgt ggttttccgg acggccggcg ctggtgcttt cgccgccggc
      241 accttcgcca ctggcgcgcg ggcgacaggc gtctctgcga ccggttgcgg cggcggtacg
      301 gccggtggcg gagagggcgc cggagttcga cgcggagcgg cgacacccgg tggtcgcagc
      361 acggtgcgat cgctgacgcc cgaggcgcgg gacgggaact ggtcgtcgag cctcgtcacg
      421 gcgtcggccg gcggcatgtc gtctgtcgat ggcgacctgt cgaacggcgt cgcggcggtc
      481 gccgccgagg cccgcgccgc gtgctcgcgc agctcgatcg ccacgttgat ctcggcctcg
      541 aggtcgatcg cgccggcgtg tgtctcgtcg aaggtcaacg cctgctgaca ggcgtcgagt
      601 gcggcctcga ggtggccctc cgcgagcaac tcgcgggcct gctgcagcgc aacctcgatc
      661 tgcgccaacc ggcgccgggc gatcgcctca cggtccgtgc gcttgggatc tggaggcggc
      721 gtcatctgcg ccacgccgac cgcatcgtcc tggcgtcgcc gcgcggatcc cgtcccgcgg
      781 gtgcccatac ccgccggagg cgtgtcgcgg ctcgccagga tggtcggggc gttccacgcc
      841 gcgttcgacg agaactcctt gcgcacgcgg ctgatcgccg ctcgcagcgc ctcggaatcg
      901 tcgtagcgtt cggccgccgt tttcctcagt gccttcacca cgatggcgat gatgtcgggg
      961 ctcacgtcgg gcgccaggcg ctccagcggc accggttctt cggtgaggat cttgtgggtg
     1021 atcatcggca gcgtgtcacc cggaaacgcc tcggaatagg cgagcagctc gtagaagacg
     1081 acgcccatcg caaactgatc ggcgcggtga tcgacgggat cgccggtgat ctgttccggc
     1141 gccatgtacc cgggcgtccc gatcatcaca gacgtattcg acgctgcgcc gaggatccgt
     1201 gcgatgccga agtcgaggat cttcagccgg ccgttccggt cgatcatcag gttggctggc
     1261 ttgatgtcgc ggtgcacgag ctgcatctgg tgggcgtagc cggcgccggc cgcaagttcc
     1321 tccatccacc gcagcttgtc cgccatcgtc accggtacct tgcgccggat cagcccggcg
     1381 acggtctcgc ccggcacgta ttccatgacg atgtacggtt gggagtcgta ggacccgaag
     1441 tcgaagacag tgacgatgtt gggatggttg agcgcggcag ccgcgcgggc ttcacggctg
     1501 aaccgttccc gcgcatcggg tacgtcgagg tccccggaga agactttgat ggcaaccatc
     1561 cggccgagca ccggatcgtg agcgacgtac accgcgccca tcccgccacg gccgagccgc
     1621 cgctggattt cgtagcgacc gagttggctg cctggctcga acatctatct acgtcccttc
     1681 ggggtccagc gcacgcgagg cgaaccgcga ggcaagtact ataaggccaa cgcgcccgaa
     1741 ggagtgcgcc ccaaagctcg ggtggaggaa agtctcctcg aggacgaact tctccgccgt
     1801 cggtgcgacg ccgacgctac ttgattaccg cctgcgccac ggcgttctcg aagtcgcgct
     1861 ggagcgccgg cgtgctcgtc tgcatcagga tgatggtcac cattccggct gccggctcga
     1921 tgtgcacgtt ggttccatag gcgccggccc agccccaggc gcccttggtg acgcgccgat
     1981 cggccgccac gggatcctcc acgacctgca cacccaggcc gaagccgata ccacggggcg
     2041 acatgcccat ctgaccgcca gccagatcgc ccgtgtggtt ggacgccatc aactcgatcg
     2101 ttcgaggact gagatatcgc ttgccgttca actcccctcc catcacgagc atcgtcgcaa
     2161 gctgcgcata gtcttcggcc gtgctcacca gcccgccggc accggagaaa taggtggcgc
     2221 tgctcaggcc cgactgatcc ggcgatcgtt cgaggccgtt cgggccgcgt cgatagagcg
     2281 ggaccaggcg aggctgaatc gccgccgtca gcgtgaagcc cgtatctttc attccaagag
     2341 gatcgaacag gcgctcgcgc aggaactgat cgaaggtctt gcctgaggcg acttccacca
     2401 cgcggctgag cacgtcgaac ccggccagcc cgctgtagcg ccacagcgtc ccgggctgaa
     2461 aatcgagcgg gacggcaccg agcttcggga tataggtagc gagggtatcg ctcggcgttc
     2521 gagctgcggc ctgacccgca acagcgtttc cgagaccgcc gctcatgagg ccagagccgt
     2581 gcgtgagcag gtcgcgaatc gtgacttcac gcgtggccga aaccaggtcc acttcaggcg
     2641 gcgggccgcc ccggcccgcc tgaggaccgg ctggaggcgc cgcgccgccc cgcgcgccgg
     2701 gccgggccac cgccaccttc gccgacttga actcggggat gaagcgcgac accggatcgt
     2761 tcaggcgaag cttcccctcc tcgagcatca tcatcaccgc gacagcggtc accggcttgg
     2821 acatcgaggc gagccggaac acgccgtccc tgggcatcgg acgcttggct tcgacgtcga
     2881 tcagcccgtg cgcctcgagg tgcgcgattc gtccgtttcg cgagacgagc gtgacggctc
     2941 ccgccagcga gcccgcgtcg atgtgccgct ggacggcctc gtgaatccgc gcgagacgct
     3001 ccgatgacag gccgacgtcc tccggcttgg ccgtcgtgat cgacccggcg tgcagcggca
     3061 gccacgcaaa cgaacacgcc agagtcacac agacgacaca cacgagcagt gacgagccga
     3121 gccggcgatg cgtcatgaga gtcctcccgg tgacgcgcat gataggaggc ggtccaccca
     3181 tggtcaagga gtggaatagc catttcggcg ggacgtccgg aaccagaggt ccaaagtctg
     3241 ctgactggcg gccttgtgag cgccctgccg gcgtcagagc gcgtcctttc gtgcagcggc
     3301 gcgcaggcat gccggttgcc acttcagggt ggcagtgacg ccgtacgcca tgacgacgtc
     3361 acggaagccc ctcctgaaag gggctcccct cctcacgccc tgatcgcttt gtccttgcgt
     3421 gtgcctggga cagggcggtc agggtgccct cctcctcgcc gctattcggc cgctcgctcc
     3481 tgaatcgact ggaggtagcg aaccagcgcc gcgatgcgtg cgctcacggt ctcgtcgccc
     3541 tccggggtgg cgcgcctgaa tgcatcgccc cacaccggca tcgttcgatc gccgtgcgac
     3601 gacacatcgc ggccgtcgat gatccgccgc acgcgctcgc ctggaaacac gtctccgttc
     3661 cgtttcgcga actgcgtgag atcggccggc gccttgcgca gcgcggacgc catcggcccg
     3721 tcgccccggc ccgcggaccc gtggcagctc gcgcagtatt ggcgaaacag cgtcgcgccg
     3781 ttctcgaccc gctgcagcga ctcgatgtga tcgacgatgc cgtcgatgcg aacatgggca
     3841 cgcgtgtcgg atgcatcgag ggccctgaac gccggtcccc acaccggcat gtcgctggcg
     3901 ccatgcgccg cgaccggccg gccgacgccg gcgatgtagg cgcgcacgcg gtcgcgcggg
     3961 tactcacccc cgtttcgttc ggcgagcgtc gtcagatcgg ccggcctggc ccgaagcgcc
     4021 gcggcgacgg gtccatcgcc gcggccgccg cggccgtggc acggggcgca gtagaacgcg
     4081 aatgactcgg tcgggccgag cgactcgatc gccagcggcg gcagctgccg ctctccttgc
     4141 cctccggcag cgagcgccat ccacgacacg cagatcgccg cagtcgcgac gatcagagca
     4201 gggccccgtg aaatttttcg tgcgcgtgcg gaagtttcca ttgttttcgc gcggattgcg
     4261 gcctcccggc acactgacag caacgttcgg gcctgccggc ggtggcatgt gttctgattc
     4321 gacgttcggc agcggatgtt cgtgttggta cggaggtgca ggcgtggcgc gcgagtcgtt
     4381 ccatctcgac ccgatcgggg ccagggcgac ggcacgccac tacattcgtg acatcgtgta
     4441 cggcgccaac gacggcctga tcacgacctt cgcggtggtg agcggcgtcg ccgggggggc
     4501 cctgtcgcag gtggccatcc tggtgatcgg cgccgccaac ctggccgccg acggcgtgtc
     4561 gatgggcgtc ggcaacctgc tggcgatccg cgcggacgag ggcgctcgcg cagcggaggg
     4621 tcttcccgag ctcgaggcgt atccgtggaa gcacggcctc gcgacgctga tcgcgttcgc
     4681 cgctgccggt gcgatcccgc tcgtccccta cgtgctgccg cttcccacgc cggtgcggct
     4741 gccatggtcg atcggtatga ccttcaccag tctcttcttg cttggcgcgg cacgcgcgct
     4801 gatcacccgc gatcgctggt ggcgcaccgg gctcgaaacc ctcctgctgg gtctcgttgt
     4861 cgccatcgcc gcctacggcg cggggcggtt ggtcgcgagc gttgtgcgga cgtcagagct
     4921 tggtccctgg tccctggtgc ttggttcttg gtccgtccct tagtccttgg tccgtccttt
     4981 agtccttggt ccgtctttag tccttggtcc gtctttagtc cttggtcctt tggtcacgga
     5041 gcggaccagg gacgaagaac cgaggacgga ccaaggacga agaacggacc aaggcccaag
     5101 aacacagcac caaggacaag caggtgctag tcaccgagcg agaggtcgta tcgacgcctg
     5161 acggccgcga tgacgtcggc gacgacgtcg cgctctccgt cgtatcgatg gcgcgcctcg
     5221 agatcgagca gctccgaggc gagcgcgtga tcgccgaaca cctcgccaat ccacctcgag
     5281 aaatcgcccc ggcggagata ggcgcgaagc gcctcgaccg gcgagcgctc gatttcggcg
     5341 acgaactctc tcaacgtcct cgcggtcctg ggcggctcgc ccccgtagtc ggcgaactgg
     5401 aaagcccggc tgtcggtgac aggaacgtct acgtacttct cacggtgccg cacgtgcgtc
     5461 gtgaggcgcc ggccgacgac gaacgggcac agcacgcccg cggcctgctc ggcgagcggc
     5521 acgatcacgg cccgtccgat ggggagatgt ccgagagtct gccgccacgt cgcgatgtcc
     5581 ccctgcgcgc agacggcgct ccgctgccac agcgcatcga cctcgacggg attcgactcg
     5641 caggtcacga taatcacctc ggtcgcctcc agcagcctcg cgggaagcct cgacgcgaga
     5701 tgcgtgacga cgacataccc gttcatgtcg aagtcgagga ggcggcccgc atcggcatcg
     5761 tgcaggaagt aatgggcttc gtcgatgacg atcctgtgcg gcaggccggt gctgcgccgg
     5821 atctcactga gggacgggag cacggcgcgc gcgtactcga tcttctgatc ctgccgcaga
     5881 tgggagagat cgatgatcgc gcttcgatcc ggataccgga gcgcccggag aagttgtcgc
     5941 ggcgtcggcg gcggctcctc tcccccgagg acggtgacgc ccgggagcgc ttcgagcgaa
     6001 cggtagtcgc cctcgggatc gatgacgcag acgcagtagc cgagtaggat cagctgctcg
     6061 cacagcaggc cggccatcca ggacttgccc gacttcgcgt cgccggcaat cagcacgtcc
     6121 cgctcgcgga cggtgagagc gatctcgcgg ccgtcctcgc tatggccgag ccgcaggtac
     6181 cggcgcgaac gggttggccg aggcagcgtc cgcgtcgcgg ccagcggcga cagaaacgcg
     6241 gcaatcgcct cgggaccggt tcccgcgacg acgagatcgg cagccgactt gagcgccccg
     6301 ctgccccact cgacagcggc gccgacctcc gcgaggcgga ggagctcgtg gtcgttttcg
     6361 gcgtcgccga ttgccagcgt gttccgggcg gagagccgca gcatgtcgag cgccgcgtgc
     6421 aagccggttg ccttgctgac gccttgggtc acgatcatca cgcgcgactg gttgaaggcg
     6481 gcgaccaggg gaagctcgag cgctcgaatc gcctcgagca gccgttggct ctcgtcggcg
     6541 tcggcatcca ccaggcagtg cccgctctga aacgggatgc cccgcctggt gatctcggcc
     6601 aggaacacct cggggatcct cggggcgagc acggtcgtgt ggtcgctttc cgggaggtgg
     6661 acgacggcgc cgttctcagc gaccacgccg tccacgaagc gcagatcccc cgccacgccg
     6721 cgaagctcgt cgagaatccg cccggtgacc agcagcacgg tcgtgccgct ggaccgcagg
     6781 gccgcaatcg actcgcggac cgatggcacg acttcgccgt cgcgggcgat cgtcccgtcg
     6841 tagtcgagcg cgagcaccgt gagtttcatc gccctcccca ctcgccattg tagaagcagc
     6901 gggagacccg ggatgccaca atggcacagg tgcttttcga cacccagcag gacgtcatcg
     6961 actgcctgat ggatcccgcc acccacggcg gctcagccgt cagccgcgtc gagacccaca
     7021 gcgccatcgt gttcctggcg ggcgcgcggg cgctgaaact caagcgcgcg gtgcgcttcg
     7081 actacctcga cttctccacc gaatctcttc gaaaggcggc gtgcgaggcc gaagtgcgaa
     7141 tcaaccgcct tgcggctccg tcgctctacc gcggagtgat tccggtcaca cgcgacgcgt
     7201 ccggctcgct ggcgctgggt ggccgcgggg ttccggtgga ctggctcgtg gacatggttc
     7261 gcttcgacga agaagggctc ttcgatcgac tggccgcgcg cggcgcgctg aacatcgatc
     7321 tcatgcctcc gctcgccgcg gcgatcgcgc gattccacga agccgccgat cggcgcgacg
     7381 accacggcgg ccgggccggg atgcagtggg tagtggacgg caacgccgcc gggttcgccg
     7441 aacagggcgc tggcgttctc tatccggctc tctgctctga actgactggg aaggcgaaag
     7501 ccgagatcga tcgcgccggg gatctgctcg atcgccgcgt ccgcgagggt cgggtccgtc
     7561 agtgccacgg cgatctgcac ctgcgcaaca tcgtgcttct cgaggggaag cccacgctgt
     7621 tcgacgccgt cgagttcaac gacgagatcg catgcgtcga cgtgttgtac gacgtggcct
     7681 tcctgttgat ggatctctgg aggcgaaggc tcgagcctca cgccaatgcc gtcttcaacc
     7741 gctatatcgc cgagagcggc gatcgctccg gcctggcgct gctgccgctc tttctgtcct
     7801 gccgcgcggc gatccgggcc aagacgagtg cgaccgcggc gcggatgcag catgacgggg
     7861 ctcgagcagg cgagctcagg gatctggctc gcgagtacct cgtgatggcc agggacttcc
     7921 tcgagcccgg ccctccgcgg ctgatcgccg tcgggggctt ctccggttcg ggaaagtcca
     7981 cgctggcgcg agccatcgct ccttcgatcg gacgcgcgcc gggcgccgtc gtcgttcgca
     8041 gcgacgaggt ccgcaagtcg ctgctcggca tgccgatcca ggggcgcctc ggacccgagg
     8101 gatacaccgc cgacgtcacc gatcgcgtgt accgcatggt gagcgagcag gtcctcgaag
     8161 cactggaagc tgggcattcg gcggtcgccg atgccgtgtt cgcacggtcg tccgatcgcc
     8221 tcgcgatcga gcaactcgcc acgaaggccg cggtgccgtt cctggggctc tggctcgacg
     8281 cccctgctga cgtcctgtcg ggccggacgg agacgcgggg tccggacgcg tccgacgcgg
     8341 ggccgtcgat cgttcgacgg caggtcgcgg agggtcacgg cgagatcagc tggcacgtcc
     8401 tcgacgcgag cgcttcagca cacgtcgttg cggaacaggc cggcgcattg gccggcgccg
     8461 cacccgctgc aaaacagtga cgcccgagac tgacgtctcg ggcgtctcac gaacgcgcga
     8521 aatctcgccg gagcctcgcg acgtatttgg tcgggatggc gggattcgaa cccgcggccc
     8581 cttgaccccc agtcaagtgc gctaccaggc tgcgctacat cccgagtgtc ttgattacgc
     8641 tagtcgcttg cgtttcgaac ccgacgtgga cttccgggca gtgcgtttgg agacggactt
     8701 ggacacgacg cgcgctttcg gacgagccgc aagcgaccgg cgcacgcctc gtttggccgc
     8761 aaccggcgac tcgaggcggt agtcgtccac gaccaccacg gtgcccgggg cagccgacag
     8821 catcttcaag atcgatttca gcccgtcgcg cacgtgcgcg atgcgcgtcc gggcgtcgga
     8881 ggcgagcgct tcgagcacct ccgcgacttc ctcctcgctc gcaccctgct gcgccggcgc
     8941 gtcctgttcg agccgcttcc gggcccccgc aagcgtcagc ccttctccga ataccagctg
     9001 cttgatccgc tggacctgtt cgagatcgga ctgccggtag agccggggcg tctccggcga
     9061 tttctgaacc ccgatgccgg gaaattcctt ctcccatgat cgcagcacgt agggctgcag
     9121 ttgggccatc tcgcagacgt ccacggcctt gaagtacttc gaggtcactc gagatgagta
     9181 taacagcgaa cctttcctat tcggttcagc gggttcacga ggttcaacgg gtgcaggagg
     9241 tcctgttcga agggttcagt tcgatcgtgg gttggcaggt tcactgacga ccgcggcgag
     9301 gagttcgcga acccggtgaa cctttcgaac ccgttgaacc atttgaacgg cgaccgctgg
     9361 aaccccgtgc actctttgag ccccttgaac ccgttcgttg cctcgcccgg acatggatcc
     9421 gaagcggtgt cccctcgaag ccgaactcct cgcgcaggca gttctgcaga taccgctgat
     9481 aggaaaagtg aaacgacgtc gccacgttgg taaagaacac gaacgtgggc ggtgccacgc
     9541 cggtctgggc ggcatacagg atgcggacat ggcgcttccc cggactcgcg ggcggatgtg
     9601 tggcggcgat acgctcgacg agcctgttga ggtcggcggt cttcacgcgc tgccgcctcg
     9661 atcgggccac cttgtcgatc gtctcgagca gccgcggtgt tcgctccccg gtcgccgccg
     9721 aaatgtggag gaccgtggcg tagtcgagaa acttgagctg ctccctcagt ttctcgtcga
     9781 actccttcac aaaatcgggg ccccggccct tgagcagatc ccacttgttc gcggcgacga
     9841 tgaccccgcg cccgagacgg tcggcctcgc cggcaatcgc cgcgtcctgg tcggtggctc
     9901 caacggacgc atcgacgacc acgacgacga cgtcggcggt ttcgatcgcg cgcctggcca
     9961 ggagcacgct gacggtttcg acctgccccc cccgcgccac gcgtcccggc cgccggatgc
    10021 cggcggtgtc cacgatgcgg aagctcctgc gatgccatgc gaggacggtg tcaacggcgt
    10081 cccgagtcgt gcccggcgcc tcgttgacga tcatgcgctc ctcgcgaagc aaccggttga
    10141 ccagcgaaga cttgccagcg ttgggcctgc cgacgatcgc cacggccact tcgtcggcca
    10201 ccggaccggc tgccgacggc gcagacgtat gcggcagcgc ctcgacgatc gcgtcgagca
    10261 gatccccgac gccgtcgcca tgctcggcgg atacctcgag gacgggatcg aacccgagct
    10321 ggtacatatc gatgacgcca ctggcggcgc gacggtcgtc catcttgttc accgccacga
    10381 gagccggctt tccggcgtcg tgcaccactt tcgagatgtc gcggtccccc gccaccagcc
    10441 cttcgcgtcc atccaccacg agcacaagca gatcggcgtc acggatggcc cgtcgtcccc
    10501 gctcgagcac cagcgcgtgc agcggatcct cgctcgctcc gaacatgcct cccgtgtcgg
    10561 tgagctcgaa acgcgcgccg cgccattcga cgggctgcgc gatgacgtcg cgtgtcgtcc
    10621 ctggaacggt cgtcacgatg gcccgccgcg tcttcgtcac tcggttgaac aaggtggact
    10681 tcccgacgtt cgggcgcccg atcagcacga cggaggcaag agatctcggc acttctttga
    10741 ctgttgttga caggctaagt gactatttct atagtggtta cagagttttc tggacgaggc
    10801 gcccacgcat gatcaaggtc gacattgtca acgaggtctc gaagatcgcc gacatcacca
    10861 aggtcaaggc tgaggtggcg gtcgacgccg tcttcgacgc catgcgtttg tcgatgcagc
    10921 gcggcgaccg aatcgagctg cgcgggttcg gcgttttcca ggtgaagccg cgcaagcgag
    10981 gcatcggcag gaaccctcgt acgggtaagg aagtgcgcat cccgcccggc cgcacgattc
    11041 gcttcaagcc cggcaaagac ctgcagaata tcggataaca cgcccgcatg ggctctccat
    11101 ttccatcaaa gccgccagac gtccgccttc gggcggagcc gccgtccgcg caggagccgg
    11161 tctacgtgat gccggcggcg ccgaagccgg agcgcgtctg gcggtatgtc gtgctctttg
    11221 gcctgacgct cgtcaccacg accctggtgg gcatgggtca ttacgagagc ttctacaaag
    11281 gcttccagtc cgtcgaactg ccgttctccg actgggagct cgcgactcgc ggcctttggt
    11341 acagcacgtc gatcctggcg attctcggcg ctcacgagtt cggacactac tatgcgtgcc
    11401 ggtactacgg cgtgagcgca tcgcttccgt attttctccc cgttcccttc gccctgaccg
    11461 gtacgctcgg agcgttcatc cggattcggc aaccgattcc gagcaagcgc gagctgttcg
    11521 acatcggcat cgcgggacca atcgcggggt ttctggtcgc cgtgccggtg ctcatgatcg
    11581 gcatgtatct ctccacggtc gtgcagctgc cacaggactt cgacggtctc gtgttcgagc
    11641 tcggcgaacc gctgctcttc caggcgtcgg cctggctgac gttcggcacc gtggccgagg
    11701 gctacacggt caacatgcac ccgatggcgt ttgccgcgtg gttcggcctg ctggccaccg
    11761 cgctcaatct cttcccgatg gggcagctcg acggcggtca cattgcgtac gccgtgctcg
    11821 gccgtcgaag caccctggtt acggcaggga ctgttctctc cctcgtcggc ctgtcgttcg
    11881 tctcatcgtc gtggattgtc tggacggtcc tgacgatctc gatgctggtc gtgttcggcc
    11941 cccgtcaccc gcgcaccttc gacgagcacg tgccgctcga tcgtgcgcgg atgtggctcg
    12001 ccctgttcgc gctggccatg ttcgtggtgt gcttctcccc ggcgccaatc gaagtcatcg
    12061 atctcgtgcg aggcagatag cccgaatcac agcacgctca acggatcgac gtcgatcgtc
    12121 acctttctcc gcgcgtccgg cagctccgcg aggacggctt tgagcgcggt gcgcatgtcc
    12181 gatcgccgcg cgcccttcag gaagaactgc acgcggtgtt cgccgcgcag ccgtacgagc
    12241 ggcgccgggg ccgggccgag aatcgtgaag ctggacgcct cgcccgcttt ccgcagccgg
    12301 cccacgatgt cgttggccgt cgtcatcgca tcgtcgaacg accggccgcg cgcgaccacg
    12361 ttgacgagcg ccacggcggg cgggtaacgc atgctgcggc gatacgccag ctcgcgctcg
    12421 aagaacgcgg caaagtcctg ccggcacgcg agctgaatgc tgtagtgctc cggatagagc
    12481 gtctgcacga ccgcctcgcc ggcccattca ccgcggccgg ctcgcccggc gacttgtgtc
    12541 aggagctgaa acgtccgctc gccggcccgg aagtcggcca cgccgaggcc gacgtcggcc
    12601 gagatcacgc cgaccagcgt cacgcgcggg aagtcatgac ccttggcaat catctgcgta
    12661 ccgacgagca catccagatc gcccgaggcg aacctcgcca ggatcgacgc gagcgcgccc
    12721 ttccggcgta ctgagtcgcg atcgacgcgg ccgatgcggg ccgccgggaa ccgatcgcga
    12781 aggtgctgct cgactttctc ggttccaaag ccggtctgct cgaggtaggg cgccgcgcat
    12841 ttccggcacg ccttcggcac catcgtcgag tagttgcaat agtggcagcg cgcgcgccag
    12901 ccttgcctgg cgcggtgcac cgtcaacgag atcgtgcagt tcgggcagtc gaaggcgtca
    12961 ccgcactgcc ggcagaacac cgccgtcgag tatccccggc gattgagcag cacgagcacc
    13021 tgctcgctgc gcagcaaccg cgcctcgatt gccgatgcca gctcgcgcga gatgacgaca
    13081 tccggcccct gctcggcgta ctcctcgcgc atgttcacca accgcaccga cgccaggggt
    13141 cgatcgagga cacgccgttc cagcgtggcc agcgtgtact tgccagagac ggcgtgctga
    13201 tagctctcca tcgacggggt ggccgagccg agcacgacga gcgcgttctc ccggctgccg
    13261 cgcaggatcg cgacgtcgcg gccgtggtac cggggcgttt cttcctgctt gtacgacccg
    13321 tcgtgctcct cgtccacgac gatgatcccg agcctggtca gcggggcgaa caccgcggat
    13381 cgggtgccga cgacgacatc gacgtcgcct cgccggatgc ggtgccactg atcgtgccgc
    13441 tcaccgtccg ccaggccgct gtgctgaatg gccacgcgat cgccgagagc cgcgcggaag
    13501 aggccggctg ccgacggcgt caacgcaatc tcgggaacga gcagcagcgt ctggcgtccg
    13561 gagcgccggg cgcgatcggc caggcgcagg tagatctccg tcttaccgct gcctgtcacc
    13621 ccgtgcagca gcgcgacgcg gaactcttgc gcatcggcca gggccgcgag cgtctcgagg
    13681 gccgtcgcct gctcggccgt gaggtcgcgt gatgcccagg cggcgggcat cgccgctctc
    13741 ccgaacgggt cccggtccgt atcctccgtg cgaatcgtga cgaggccgcg tgccgccagg
    13801 cgtcccacca cgtccgccgt cacgccgcga tcgcgcagct ccgacagtgg aagtccaact
    13861 ggtgcggcag ccaacacctc gatgacctgc cgttgccgct cggtgaggcg tcccacgtca
    13921 gggctcagcc cgtgggccgt gaccgcggcg acgcgatggg tccggaacga ggtcggcttc
    13981 cgccgcgcgc cgggcggcat ggcggaggcg atggcatcac cgacgccggc gatgtagtag
    14041 tcggcaaccc agcggcacaa ctcgacgatg tgaggcggga ggagcggctc gaagtcgatc
    14101 gtctcgacca cgtccttcaa cgcattggtc ggcgcctcac tcgacgatcc gtggcccacg
    14161 acgcagccgg tgagcgtgcg cgagccgacg ggcacccgca cgcgtccgcc aatcggcgga
    14221 agcacgatgt gattcggtac gttgtagctg aggagatcga gaaacggaac ggggaccgcg
    14281 acggacacct ggcgcacgat cagctcatga ttccaggatg gctcgaactc gtttcatatc
    14341 ctcccacact tcacgcttgg ccgaggggtt gcgcaacagg tacgcggggt gataggtcgg
    14401 cagcaggatc gccgatcggt acttgtactc gcggccgcga ctgcgcgtga tcggatcggc
    14461 cgtccggagc agcgactggg cggcgaattt tcccagcgcg acgatcacct tcggcttcag
    14521 gacgtcgatc tgccggaaca ggaacggctc gcagcgctcg acttcgtcgg gctcggggtt
    14581 gcggttgttc ggcggcctgc acttgatgac gttggcgata tacacgtcgc cgcgcgtcat
    14641 gccgatcgct tcgatgatct tcgtgagcaa ctggcccgcg cggccgacga acggctcacc
    14701 ctgaatgtct tcgtccccgc cgggagcttc cccgacgaac atcagatcgg cattcgggtt
    14761 gcccacgccg aacacgacct gccgccggcc gagcgtgtgc agcttgcagc gggtgcattg
    14821 ggggccgatt tcgatgcgca gagcggcaag cgcgtctgcc tgggatgaga acacttggag
    14881 gggctcggcg ggttcaaccg gtttagacgg ttcagcgggt tcgagagatc cgactggccg
    14941 ctgcgcggcc gcactctcag ctgcgggtcg ggaccgccac acagcctccc gccgaatccc
    15001 ttcaacaccg agttcggcga agaactcgag atgcgcggtc agctcgtccg acaccggcgt
    15061 cagcgggagg tggccgccga agcgcgcacg cgcagcacgc gttcgacccg atcgaggatc
    15121 gcggcggcga tccggtcctt gctctgcagc gggacgctct cctcgccctc ctcgccgacc
    15181 agcgtcacgg cgttggtgtc ggtgtcgaag ccctggtccg gccgcgacac gtcattggcg
    15241 accacgaggt ccacgagctt gcgcgcgcgc ttctcgcgcg ccttcgcgag caggtcgtcg
    15301 gtctcggcgg caaaacccac gagcaccggc gtcgtcaatc cagaacgggc gcgtgccgcg
    15361 ccgagctcgg ccaggatgtc cttcgtgcgc gtcagcgtca gcgtgagcgg cgcctcggcc
    15421 ttggtcagct tgccgggcgc ggcggccgac ggcgcataat cggcaacggc cgccgccatg
    15481 atcaccacgt cggcgtggga ggcggccgcc gtcacggccg cgtgcatctc ggcggcgctg
    15541 cggacgcgca ccacctcgtc cacggccggc ggatcgagac gcgtcggccc tgcgacgagt
    15601 gtgactcgcg cgccgcgccc ccgcgcctcg gccgcgagcg caaaccccat ccggccgctc
    15661 gaacggttgc cgatgtaccg caccgggtcg atgtcctcgt aggtcggacc cgccgtgacg
    15721 agcacatggc ggccgcgcag cagcgaatcc ggaggcgcca gcatccggca cgcggcgtcg
    15781 acgatctcgt cgggctcggc cagacgtccc ttgccgatcc agccgcaggc caggtacccc
    15841 tcgccgggct cgacgaaccg gacaccgcgc gacgcgagcg tctgaaggtt ccgctgcacc
    15901 gcctcgtgcg cgagcatgtt cgtgttcatg gcgggcgcca gcagcacggg cgcccgcgtc
    15961 gccaggtaga gcgaggtcag gaagtcatcg gcgatgccgc cggcgaactt gccgatgacg
    16021 ttggccgtgc agggcgcgac gagcagcagg ttgatgccgt cggcgatcgc gacgtgctcg
    16081 atgtcggcgt tcatccccgg gcgccactgc gacgtgatga cgggcctccg cgtaatcgcc
    16141 tcgaacgtga cggggcccac gaacctggac gccgagcgcg tcatcacggc cacgacgtcg
    16201 tggccgcgct tctgcagacc ccggcacacc tcgacggcct tgtaggcgcc gatgccgccg
    16261 gtcacgccga gcgcgatgag cgccacgtca gctccaggac ttctcgtccg tcactctttc
    16321 gacttttcct tcgcggactt ccttctgg
//
LOCUS       DBNGSH010000223        11924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1205897, whole genome shotgun sequence.
ACCESSION   DBNGSH010000223 DBNGSH010000000
VERSION     DBNGSH010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1205897"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="AC16CY_05130"
     CDS             complement(<1..326)
                     /locus_tag="AC16CY_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353196.1"
                     /translation="METGITPTVGPAPPEEERAASAWARLLEQASTCTDAVAFQALAR
                     KATRLEKEVGGPPGHMPARIALMGGASTDLLVRPLGLALRLVGFAPELHVTPYNVMLQ
                     EMLEPD"
     gene            complement(452..1639)
                     /locus_tag="AC16CY_05135"
     CDS             complement(452..1639)
                     /locus_tag="AC16CY_05135"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR3353197.1"
                     /translation="MSLLSETFILLSIGTACWMWLTRGPWRLAGFLLASGYYAFSYVG
                     VLGMASTLGFCLAGFGCVLAVRARPGRLWLAVCALTLAFVYARSYSFLTLVLPDGWRS
                     TILATAGLSFLFFKILHVVIDSAGGRITPSFGRYLAYCFNFTTFLLGPIQRYQSFTDQ
                     WDGRVEAIPRTFEAHLDATNRLLRGFVKKFVVAEYLFQYALQGNAPVESMSLVDLQLA
                     TYMFYVFLYFDFSGYCDIVIGIGCLLGIRPPENFNLPFLAANPSQYWLRVHESLTHWL
                     TDYVFNPLYAAALRSRPLGSRPLASMAMAVMVTMLVAGLWHGTTFSFVLFGVVHGVYL
                     VVFRTYEHVALSVLGRRGLRQLRSSRAWLVASTIITFHFTATAYIFFVLDTNGLMRIL
                     ERF"
     gene            complement(1685..2299)
                     /locus_tag="AC16CY_05140"
     CDS             complement(1685..2299)
                     /locus_tag="AC16CY_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3353198.1"
                     /translation="MSTGTLIEAVKAGDSQQVETALASGADVNAAGSEQEWPALNYAA
                     GRGDLGLVERLVQAGADVFRTGRDKRTPYQIAVAAGHAAVARYLAKAEAAAGGDKERI
                     SSRQSETRPYCKAYHLKDLRRFGAWAQVQAGPEAVAGLSDESVVFVHQDLTVTQSMFH
                     GEQVVLDRVTPDWRAFCATELGFKVPDDFDLMPPKTVADPPSGQ"
     gene            complement(2367..3416)
                     /locus_tag="AC16CY_05145"
     CDS             complement(2367..3416)
                     /locus_tag="AC16CY_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HIR3353199.1"
                     /translation="MRFSLFFFSGRWPDGPGDHYRLFRDSVQFADRSGFEAVWTPERH
                     FHRFGGLYPNPALTGAAAAVLTNRIGIRAGSVVAPLQDPLRTAEEWSVVDNLSQGRVG
                     VSFASGWNVNDFALEPESYQSRHEVMHERIDIVRRLWRGETVCRRNGIGKEVEVSIFP
                     RPYQAEIPIWITAQSDRSCVKAGEIGANLLTNLNYHKLDELRTKIALYREAFTRRDGY
                     SQEVHGRGHATVMVHTFIGDDEAIETHGKTAYAEYLRTNLDLQSERAQGLGLTVDASE
                     EDIRAVIARAVNRMIGQVGLIGTPEACLQRARGLADLGVDEVACLIDFGVEADAVMSS
                     LEHVAAIGRTFTAGK"
     gene            complement(3416..4870)
                     /locus_tag="AC16CY_05150"
     CDS             complement(3416..4870)
                     /locus_tag="AC16CY_05150"
                     /inference="COORDINATES: protein motif:HMM:NF028132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase AlgX-related
                     protein"
                     /protein_id="HIR3353200.1"
                     /translation="MERSPNIVNRWLVVVSTAVWGYLVAVLFYNSRIAGLRVSGSILL
                     CLVAGGAIAFSALARRLSERRRGDLLALCLSLLVGLLGLDTAYAVWMNLDTDRRPAQA
                     DERLNDPHVWHGELFPRSYYPSDRAFTLYKPNVRVEALTYGEFYERRMLESPTLVRDA
                     LRLRRLAYTIDANGLRNRVPIQQSKIWALGDSYAMGYSTTEGLTWTDRFAELTGQPIY
                     DVGVSGTGPGLQVQILEHLLRKEKGAARPERLLWMVFEGNDLENSYAERRSLPPAGTG
                     LRSLLDDTVVGDLLSLPSTIHERSVIGRLLEGKVRFTPPRTASREAYQIDGVPLASPV
                     YHSDRWGYCLFNPVHIEAATKGEDYVLSHPNRPRLDRSFGRMKALSEEFGFRVTVVVA
                     PSAPRVLGRDFPQMPQPTAEPYFIRYVESLAGRSGFETFDLLGALSSQDWGGILYYCD
                     DHHWNERGNDVVARLLATQFRSVDEARVSDGRVN"
     gene            complement(5192..6421)
                     /locus_tag="AC16CY_05155"
     CDS             complement(5192..6421)
                     /locus_tag="AC16CY_05155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3353201.1"
                     /translation="MPAETLSRKTKFVLYLIMAATPVLFAEALVRAYFSTQVGPSVLL
                     YGTPWSRHQEKFDPKGVEMKKAEDESSVAFHQNVTAGYSKYYPSELLFDRDEFGTRFT
                     VTINSHGFRGRDFQSAKRPGVIRIVTLGASSTFGFRSPDHQTYPVFLEQNLSSALAAR
                     HVNDASAPVTEFEVINLGIPHLTSDQIYALLAEEGLALKPDFVTFYEGINDAAWKEAP
                     DTTTERTKQAVKTVPFANQAFRALRARLLSVALIGSVISDVTTVYSSEDFQDFRKGKR
                     DRFIGNLQRLADLCQKNGIRLIVASQSATSLEFKREQLKTLTYSAEQAALRQKLAAGV
                     GLEVGEADLLIHGELMDAEREWAIANEVPYADVIAAMDGSRQNHVTWVHLNAAGNQVV
                     ASVLSEAILKELGRKGE"
     gene            complement(6450..11906)
                     /locus_tag="AC16CY_05160"
     CDS             complement(6450..11906)
                     /locus_tag="AC16CY_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIR3353202.1"
                     /translation="MDSPAGRPIPDLQVYVRDEHGELLPAGLPGEIYVAGAGVARGYH
                     GRPELTAQRFVPSPYGEGARLYRTGDGARYLADGQLGFLGRLDQQVKIRGFRIELGEI
                     EAVLIRHPYIEAAVVVAREDSPGDQRLVAYIVGKGGSPDVTTLRRHLKASLPDYMVPA
                     TFVVLDALPLTPNGKLDRRALPAPDGVRLSLGHEYVPPRTVEEEALAGIWSDALHVER
                     VGIYDNFFELGGDSIRSIGIVARAKQVGLIFSLQELFQHQTIDALSRVMMDRTSPVPR
                     SAPVREAFALMTAADRVQLPADAVDAYPLARIQSGMLFHSQLNPESGVYHDVFGYRIR
                     SLFDEQRFRAAVDVVVRRHPILRTSFHWSEFSEPLQVVHRSVPLPLEVYDLGNRPDAE
                     QAKTFDVWFEAEKTRHFDMAQAPLLRWRVHLRSDATWHFTLSFHHAILDGWSVALLNV
                     ELFREYLSSEGLAERPQRESPAASYADFVALERSTLRSEDARRFWIDRLEDLEVTKLP
                     RWPDAREAPTDRPLRVHRVRPSAEVLAGLRQLARTAGVPLKSVLLAAHFKVQSAFAAN
                     HDVVTGLTTNGRPEELEGEQVVGVFLNSVPMRMALKGGTWTDLVHATFQTERELLPFR
                     RYPLPEIQQALGGQPLFEVLFNFTHFHVYQNLADLGDAEVLETKFFERTNFTLVANFA
                     LALNASELELRLEYDGSELQEAQVARIGAYYASTFAAMVDDPQARYEDHSPLPNEERR
                     QLLEEWNDTAVAYPSDTCIHEWFERQVTTGPDRTALIDDGHLVTYRALSARANRLSNH
                     LRGLGVGPEVRVGLCMERSAELVVGILGILKAGGVYVPLDPAYPKERLAFMVADAGLE
                     VLVTEERYVPGLPDTSARVISLDASREAISREREVAPAVEVSADNAAYVIYTSGSTGK
                     PKGVEVPHRGVLRLLFGGDYVTLGSDVVIPHLSSPTFDASTFEVWAPLLHGGQCVLYP
                     ERVPTPLSLARAIQKHGLNTMWLTSALFNAVVDEAPGLLAGVDQLLIGGEALSLNHVQ
                     RALAALPSTTIINGYGPTESTTFACCHPIPVALRTTTQSIPIGRPIGNTTVNVLDAHM
                     QPVPLGASGEVLIGGAGLARGYLDRADLTAERFVPDPYGAHTGGRVYRTGDVARYLPD
                     GNVEFLGRVDHQVKLRGFRIELGEIEAVLRDHPALRDAVVVVREDAPGQARLVAYTVA
                     HAQSPDVSEVRNYLKASLPDYMVPSAFVALDALPLTPNGKVDRRALPDPSPERPATPE
                     SLVAARSPQEEIISGIWAEVLGIERVGVHDDFFEFGGHSLLATQVMSRVRNAFQVELP
                     LRVLFEAPTIAGLSEKLTAAIRANRELDSPPIVPRSREGELPLSFAQQRLWLLDRMRP
                     GDSSYNVSAAIRLRGRLDAAALDQTFSEICRRQDVLRANFVDVDGRPVQVNRPARPLV
                     LPLVDLSNLEGPAREAQATDLVERETARSFDLARDSLIRLHLLRMGDEDHVLLFSTHH
                     IICDGWSVGVIVGELSSLYVRFTGGGPSSLEELKVQYSDFAGWQRDWLQGKALEAQLS
                     YWRTQLGADPPRLALPTDRPRPQVETLQGQTYTVVLPASLTTRLKALSRAEGVTTFMT
                     LLAAFKAQLQRYTGQDDIVVGTDMANRNRQEIEGLIGFFINLLVLRTDLGGNPSFRQL
                     LKRVREKALEAYAHQDLPFDRLVEELQPERHLSDTPLFQVLFVLQNTPTSDLRLGGLL
                     VQPVPLPSTTSKFDLAVFVSEGDAGISVTWQFKTDLFERSTITRIAQHFERVLESVVD
                     DPEQPIGRLRLVEEKKKPERIRRARGTLPVDTVH"
BASE COUNT         2269 a         3892 c         3653 g         2110 t
ORIGIN      
        1 ctgtccggct ccagcatctc ctggagcatg acgttgtacg gcgtcacatg cagctccgga
       61 gcaaacccga cgaggcgaag ggccaggccc aatggccgaa cgagcagatc ggtcgacgcc
      121 ccgcccatca gcgcgatccg cgcgggcatg tgaccgggtg gtcctccgac ctccttctcc
      181 aatcgcgtgg ccttgcgggc cagcgcttga aacgcgaccg cgtccgtaca cgtcgaggcc
      241 tgctccagaa gacgcgccca ggctgacgcc gcgcgctcct cctcgggcgg tgccggtccg
      301 acggttggtg taatccccgt ttccacgact accggttcac acgctgctca gaacccacgg
      361 tatacgaaat cgacttcgct gctcgcgaac gtcacgagaa gaaagatgag gatccacgtc
      421 cacagcagtt tcgctgtcag tctcaggcgc atcaaaagcg ctccaggatc cgcatgagtc
      481 cgttggtgtc gagcacgaag aagatgtacg cggtggccgt gaagtgaaac gtgatgatcg
      541 tcgaggcgac gagccacgcg cggctggatc tcaattgccg cagcccgcgg cggcccaaca
      601 ccgagagcgc cacatgttca taggttcgaa acacgacaag gtagacgccg tgtacgacgc
      661 cgaacaggac gaaactgaac gtcgtgccgt gccagagacc agccacgagc attgtgacca
      721 tgacagccat cgccatcgac gccagtggcc tgcttcccag cggcctcgaa cgaagggctg
      781 ccgcatagag cgggttgaag acgtagtcgg tcaaccagtg cgtcagtgat tcgtggacgc
      841 gaagccagta ctgggaagga ttggccgcca ggaacggcag gttgaagttc tcgggtggcc
      901 gaatcccaag tagacagccg atgccgatca cgatgtcgca gtacccggaa aagtcgaaat
      961 agaggaacac atagaacatg tacgtcgcca gttgcaggtc gaccagcgac atggactcga
     1021 ccggggcgtt gccttgaaga gcgtactgga agagatactc ggcgacgacg aatttcttga
     1081 cgaagccccg gagcaggcga ttggtcgcat cgagatgcgc ctcgaacgtg cggggaatag
     1141 cctccaccct gccgtcccac tgatccgtga aggactgata ccgctggatg ggaccgagca
     1201 ggaacgtcgt gaagttgaag caatacgcga gatatcgccc gaacgacggc gtgatcctgc
     1261 cgccggccga gtcgatgacc acgtgcagga tcttgaagaa caggaagctg agtcccgccg
     1321 tggcgagaat cgtcgatcgc cacccgtcgg gcaagaccag cgtcaggaag ctgtagctgc
     1381 gggcgtagac aaatgcaagc gtcagggcgc acacggccag ccagagtctc ccgggacggg
     1441 cgcgcacggc gaggacgcaa ccgaacccgg ccagacagaa gccgagtgtc gaggccatgc
     1501 caagtacgcc aacgtagctg aacgcgtagt acccgctcgc gaggagaaac ccggccaaac
     1561 gccacggacc gcgggtcaac cacatccagc atgcggtccc gatcgacagc aggatgaagg
     1621 tttctgaaag cagggacaag tgtttccacg agaggcgtta cgtgaatgac gcgggcctcg
     1681 ggactcactg gccggaggga ggatccgcca ccgtcttcgg cggcatcagg tcgaagtcgt
     1741 ccggcacctt gaaccccagt tccgtggcgc agaacgcgcg ccagtccggc gtcacgcggt
     1801 ccagaacgac ctgctcaccg tggaacatgg actgcgtgac cgtcagatcc tggtgcacga
     1861 agacgacgct ctcgtccgag agacccgcca cggcctcggg tcccgcctga acctgcgccc
     1921 acgcgccaaa gcgtcgcagg tccttcaggt ggtaggcctt gcagtacggc cgcgtctcgc
     1981 tctgccggct gctgatccgt tccttgtcgc cgccagcggc cgcctcggcc ttggccaggt
     2041 aacgcgcgac ggccgcgtgt ccggccgcca ctgcgatctg atacggtgtc cgcttgtcac
     2101 ggcccgtcct gaagacatca gcccctgcct gcaccaggcg ttcgaccagg cccagatcgc
     2161 cccgaccggc ggcgtagttc agagccggcc attcttgttc tgacccggcg gcattgacat
     2221 ctgctccact ggccagcgcg gtctccacct gctggctgtc gcctgctttc accgcctcga
     2281 tcaatgttcc tgtactcacg ttcttctcct ccgtgccggc ctcgtcgaac cgggccaccc
     2341 cgggccgggc tcggtgaccg ttcttctcat ttccccgctg tgaaggttcg cccgatcgca
     2401 gcgacatgtt cgaggctcga catcaccgca tcggcctcga caccgaagtc gatcagacac
     2461 gccacttcgt ccacgcccag atccgcgagc ccccgcgcgc gttggagaca tgcctccggc
     2521 gtgccgatga gcccgacctg gccgatcatg cgattcacgg ccctcgcgat gactgcgcga
     2581 atgtcttctt cgctcgcgtc gaccgtcaaa cccagtccct gggcacgttc cgactgaaga
     2641 tcgaggttgg tacgaaggta ctcggcgtac gcggtctttc catgggtctc gatggcctcg
     2701 tcgtcaccga tgaaggtgtg caccatgacg gtggcatgtc cacgtccgtg cacctcttgg
     2761 ctatacccgt cccggcgggt gaaggcctca cgatagagcg cgatcttcgt acgcagttcg
     2821 tcgagcttgt ggtagttcaa gttcgtgagc agattggcgc cgatctcacc cgccttcaca
     2881 cagctccggt cggactgcgc cgtgatccag atcgggattt ccgcctggta cggtcggggg
     2941 aaaatcgaga cctcgacctc cttgccgatc ccattgcgcc ggcacacggt ctcgcctctc
     3001 cacagccgcc gaacgatgtc gattcgctcg tgcatgacct cgtgccgcga ttggtagctc
     3061 tcgggctcga gcgcgaagtc gttcacgttc cagccggatg cgaacgagac acccacgcgt
     3121 ccctgcgaaa gattgtcgac gaccgaccat tcttccgccg tacgcagcgg atcctgcagc
     3181 ggcgccacca cgcttccagc ccggatgccg attcgattcg tgagaaccgc tgcggccgct
     3241 ccggtcagtg ccggattggg gtacaggcca ccgaatcggt ggaagtgtcg ctcgggggtc
     3301 cagaccgcct cgaatccact gcgatcggcg aactggacac tgtcccggaa caaccgatag
     3361 tgatcgcccg gcccatccgg ccatcgcccg gagaagaaga acaggctgaa gcgcatcaat
     3421 tcacccgccc gtcgctgact ctcgcctcgt caacagatcg aaactgcgtt gccagaagcc
     3481 gggcgacaac gtcgttcccg cgctcgttcc agtggtggtc gtcacagtag tacaggattc
     3541 caccccagtc ctgggaactc agcgccccga gcaaatcaaa cgtctcgaag ccggagcgcc
     3601 cggccagact ctcgacgtac cggatgaaat acggctcggc cgtcggttgc ggcatttgag
     3661 gaaagtcgcg cccaagcacc cggggggcgg atggcgccac cacgaccgtc acgcggaagc
     3721 cgaactcctc gctcagcgcc ttcatccgcc cgaaggatcg atccaggcga ggtcgattcg
     3781 gatgggacaa cacgtagtcc tctcccttcg ttgcggcctc gatatgcacc gggttgaaca
     3841 gacagtatcc ccagcggtcg gagtgataga ccggcgacgc caacgggacg ccgtcgatct
     3901 ggtacgcctc ccgcgaggct gtccggggcg gcgtaaagcg cactttgccc tcgagcagac
     3961 gtccgatcac gcttcgctcg tggatcgtcg atggcaacga gaggagatcg ccgacgaccg
     4021 tgtcgtcgag caacgaccga aggcccgtcc ctgctggcgg cagcgatcga cgctcggcgt
     4081 agctgttctc caggtcgttg ccctcgaaca ccatccacag cagccgttcc ggcctggcgg
     4141 cgcccttctc cttgcgcagg agatgttcga gtatctgcac ttgcagaccc ggccccgtgc
     4201 ctgacacacc gacgtcatag atgggctgtc cagtcaattc ggcgaaacgg tcggtccagg
     4261 tgagtccttc cgtcgtcgag taacccatgg cgtaggagtc gccaagtgcc cagattttgc
     4321 tctgctggat cgggacccga ttgcgaagac cattcgcgtc gatcgtgtat gcgagccgcc
     4381 tgagccgcag ggcgtcgcgg acgagcgtcg gcgattcgag catcctccgc tcgtagaatt
     4441 cgccataggt cagcgcctcg acccgcacat tcggcttgta gagggtgaag gctcggtcgg
     4501 acggatagta ggacctcgga aagagctccc catgccaaac gtgcggatcg ttcagccgtt
     4561 cgtcggcctg cgcgggtcgt cggtccgtat ctaagttcat ccagaccgcg tacgccgtat
     4621 ccaagccgag gagcccgacg agaagcgaca ggcagagggc caggaggtcg cctcgtcgcc
     4681 gctcggagag ccgccgagcc agcgccgaga acgcgatagc accgccggcc accagacaga
     4741 gcagtatgga cccgctgacc ctgagtccgg cgatacgact gttgtagaaa agaacggcga
     4801 caagataacc ccagaccgcc gtggaaacca caaccagcca gcgattgacg atgttcgggc
     4861 tccgttccaa tgttgtgcag ttgtgcccgt gtacaggcta ccgccctctc gcttacagtg
     4921 cggcacgagg taagaacacc tcgcggacct gcccaagcga cgaaggccac gcctgtctac
     4981 ggaagaacta tgccgacggg ctcctcgtgg caggcggctg cctgtccccg taagtcattg
     5041 attaccaagg tataatcacg tgggttgaag atcaggagta gttgaggtcg accacaattc
     5101 tcctcgaagc agcagacttt ctactgccac cttgagatat gacgaacggg accagatcgc
     5161 cgccccgagt ggccgtctgg ggtggggctg gctattcgcc cttgcgtccc agctccttca
     5221 ggatcgcctc ggacaggacc gacgccacta cctgattgcc ggccgcgttc agatgcaccc
     5281 aggtgacgtg gttctgccgg ctaccgtcca ttgccgcgat tacgtctgca tatggaacct
     5341 cgttggcaat cgcccactca cgctcggcat ccatcaactc tccatggatc aagaggtctg
     5401 cctcaccgac ctcgaggccc actcccgccg ccagcttctg cctcaacgcc gcctgctctg
     5461 cgctgtacgt gagagtcttg agctgctcac gcttgaattc gagtgacgtg gccgattggc
     5521 tggcaacaat caatcggatt ccgttcttct ggcagaggtc ggccaatcgc tgcagatttc
     5581 caatgaaccg gtcccgtttc ccctttcgaa agtcctggaa atcctcactc gaatagaccg
     5641 tggtgacgtc agaaatcacg cttccgatta acgcgacgct gagcaagcgg gctctcaggg
     5701 ccctgaacgc ctggttggca aagggcacgg ttttgaccgc ctgcttcgtt cgctcggtcg
     5761 ttgtgtcggg tgcttctttc caggcagcgt cgtttattcc ctcgtagaac gtcacgaagt
     5821 ccggtttgag tgccaacccc tcttctgcca acagcgcgta gatctgatct gaagtgaggt
     5881 gagggattcc aagattgatg acctcgaatt ccgtgacggg cgccgaagcg tcattcacgt
     5941 gccgcgccgc gagggcgctg ctcaagttct gttccaggaa gaccggatag gtctggtggt
     6001 ccggcgacct gaagccgaac gtcgacgaag cgccgagcgt aacgatgcga atgacccctg
     6061 gccgcttggc tgactgaaaa tcgcgtcccc tgaacccgtg cgagttgatc gtgacggtga
     6121 aacgcgtgcc gaactcgtct ctgtcgaaca acagctcgct cgggtagtac ttcgagtagc
     6181 ccgcggtgac gttctgatgg aatgcaacac tgctctcgtc ctccgccttc ttcatttcga
     6241 cccctttcgg gtcgaacttc tcctgatgtc tcgaccaagg tgtgccatag agcagcacac
     6301 tgggtccgac ctgcgttgag aagtaggcac gtacgagtgc ttcggcaaag agcacaggag
     6361 tcgcggccat gatcaggtac aagacgaatt tggttttcct actcagtgtt tccgcaggca
     6421 ttcgccactc ctcctacgca gccaggacac tagtggactg tatcaactgg aagagtaccg
     6481 cgagcccgcc tgatgcgctc gggcttcttc ttctcctcga ccagacgcag cctgccgatt
     6541 ggctgctccg ggtcgtcgac aacgctctcc agcacccgct cgaagtgctg ggcgatccgc
     6601 gtgatcgtcg acctttcgaa gaggtcggtc ttgaactgcc acgtcaccga gattcccgca
     6661 tcgccctcgg acacgaatac cgccaagtcg aatttggaag ttgtcgacgg caagggaaca
     6721 ggttgcacga gcagcccccc gagcctcagg tccgacgtgg gggtgttctg caggacaaag
     6781 agcacttgga acaggggtgt atcgctcagg tgacgctcag gctgcagctc ctccaccaat
     6841 cggtcgaacg gcaggtcctg atgggcgtac gcctcgagag ccttctcacg gacgcgtttc
     6901 agaagctggc ggaaggacgg gttgccgccg agatccgtcc gaagcacgag gaggttgatg
     6961 aagaacccga tcagtccctc gatctcctgg cggttccgat tggccatgtc ggtgccgacg
     7021 acaatgtcat cctgccctgt gtaacgttgg agttgcgcct tgaaggccgc cagcagcgtc
     7081 atgaatgtcg tgacgccctc cgctcgactc agtgccttca gcctggtggt caggctggcg
     7141 ggtagaacca cggtgtacgt ctgtccctgg agtgtctcga cctgaggtcg cggccggtcg
     7201 gtcggaagtg cgagcctcgg cggatcggca ccgagctgcg tcctccagta ggaaagttgc
     7261 gcctcgaggg ccttcccctg cagccagtcg cgctgccaac cggcgaagtc actgtactgg
     7321 accttcaact cctcgagtga ggagggcccg ccgccggtga aacggacgta caggctcgac
     7381 agctcgccga cgatgacgcc gaccgaccag ccgtcacaga tgatgtggtg agtcgagaac
     7441 agcaggacgt ggtcctcgtc gcccatccgc agaagatgaa gccggatcag cgagtcacgg
     7501 gcgaggtcga aggaccgcgc ggtttcacgc tcgacgagat cggtcgcctg cgcttcacgc
     7561 gcgggccctt cgaggttgct gaggtccacg agcggcagca cgagcgggcg agcaggccgg
     7621 tttacctgca caggcctgcc gtcgacatct acgaagttcg ctctgaggac gtcctgacgc
     7681 cggcagatct cgctgaatgt ctggtcgagc gccgcggcgt cgagacgacc gcgcaggcgt
     7741 atggctgcgc tgacgttgta gctcgagtct ccaggcctca tgcgatcgag cagccagagc
     7801 cgctgttgag cgaacgagag cggcaactcg ccttccctgg accgcggcac gatcggtgga
     7861 gaatccagct ctcggtttgc ccggatcgcc gccgtcagct tctccgacag gccggcgatc
     7921 gtgggtgcct caaagagcac ccgcaatggg agctcgacct ggaacgcatt gcggacgcgc
     7981 gacatcactt gtgtcgcgag gagcgaatgg ccgccgaatt caaagaagtc gtcgtggaca
     8041 cccacgcgct cgatgccgag aacctcggcc cagatgccgc tgatgatctc ttcctggggg
     8101 ctccgggcag cgacgagact ctccggcgtc gccggtcgct ccggacttgg atccggcagc
     8161 gcacgccgat cgaccttgcc gttgggcgtc agcggcaggg catcgagcgc cacgaacgcg
     8221 gacggcacca tgtagtcggg cagcgacgcc ttcagataat tccgtacctc gctcacgtcc
     8281 ggcgactggg catgcgccac cgtgtaagcg accagccgcg cctggccagg cgcgtcctcc
     8341 cgcaccacga caaccgcatc ccggagggcc gggtggtcgc gcagcaccgc ctcgatctcc
     8401 cccagctcga tgcggaagcc acggagtttc acctggtggt caacccgtcc caggaactcg
     8461 acgtttccgt cagggaggta gcgagccacg tcccctgtcc ggtaaacgcg cccgcctgtg
     8521 tgcgcgccgt acggatcggg gacgaaccgc tcggctgtca agtctgctcg gtcgaggtac
     8581 ccgcgagcca atcccgcccc cccgatgagc acttcgcctg aggctcccag tggcactggc
     8641 tgcatgtgcg cgtcgaggac attgaccgtt gtgttaccga tgggacgacc gatggggatc
     8701 gattgggtcg tcgtccgaag agcgaccgga atcgggtgac aacaggcgaa ggtcgtgctc
     8761 tcggtcggcc cgtagccatt gatgatggtc gtggacggaa gcgctgcgag cgcgcgttgc
     8821 acgtggttca gggagagcgc ctcaccgccg attaagagtt ggtccacgcc tgcgaggaga
     8881 ccgggggctt cgtccacgac cgcgttgaac aaggcggagg tcagccacat cgtgttgagg
     8941 ccgtgtttct gaatcgccct cgccagactc aggggcgtcg gcacccgttc cggatagagg
     9001 acacactgcc cgccgtgcag caacggcgcc cacacctcaa aggtcgaggc atcaaaggtc
     9061 ggcgacgaca aatgagggat caccacgtcg ctgccgagcg tgacgtaatc cccaccgaac
     9121 agcagtcgga gtacgccgcg atgcggtact tcgacgccct tcggtttgcc cgtcgaaccc
     9181 gacgtgtaga tcacgtacgc ggcattgtct gcgctcacct cgaccgccgg cgccacctca
     9241 cgctcccgcg agatcgcctc ccgcgaagcg tcaagactga tcacgcgcgc actcgtgtcg
     9301 ggcaggcctg gcacgtacct ttcctcagtc accagcactt cgagacccgc gtctgccacc
     9361 atgaacgcga gccgctcctt cggatacgcc ggatccaacg gcacgtacac gccgcccgcc
     9421 ttgaggattc ccaggatccc gaccaccagt tccgccgatc gctccatgca gaggcccacg
     9481 cggacctcag gccccacacc caacccgcgg aggtgattcg acagccgatt cgcgcgcgcg
     9541 ctcaacgccc ggtaggtcac caggtgtccg tcgtcgatca acgcagtccg atccggacca
     9601 gtcgtcacct gtcgctcgaa ccactcgtgg atgcacgtgt cactcggata cgccaccgcc
     9661 gtgtcgttcc actcctcgag caactggcga cgctcctcgt tcggaagcgg tgagtggtct
     9721 tcatagcgcg cctgggggtc gtccaccatc gccgcaaacg tcgaggcgta gtacgcaccg
     9781 atgcgagcga cctgagcctc ctggagctcg cttccgtcgt attccagcct caactcgagc
     9841 tcggacgcgt tgagcgccag ggcaaagttc gcgaccagag tgaagttcgt tctctcgaag
     9901 aacttggtct cgagcacctc cgcgtctccc agatccgcca ggttctggta cacgtggaag
     9961 tgtgtgaagt tgaagagcac ttcgaaaagc ggctggcctc cgagggcctg ctgaatctcc
    10021 ggcaggggat agcgtcggaa cggcaacagc tcgcgttccg tctggaacgt ggcgtgcacg
    10081 aggtcagtcc acgtgcctcc cttcagtgcc atgcgcatcg gcacgctgtt caggaagaca
    10141 cccaccacct gctcgccctc gagctcctcc ggccgcccgt tcgttgtcag acctgtcacg
    10201 acatcgtggt tggcggcaaa ggcgctctga accttgaaat gcgccgcgag caacacgctc
    10261 ttgagcggta cgcccgccgt gcgggcgagc tgccgcaagc cggccaggac ctccgccgac
    10321 ggtcgtaccc ggtgcactcg aagcggcctg tcggttggcg cctcgcgcgc gtcaggccag
    10381 cgcggcagct ttgtgacttc cagatcttca agccggtcga tccagaaccg gcgagcgtcc
    10441 tcagacctga gcgtggaccg ctccagcgca acgaagtcag catagctggc cgcgggtgat
    10501 tcacgctgtg gacgctccgc gagcccctct gatgacaggt attcgcgaaa cagctccacg
    10561 ttgagcagcg cgacgctcca cccgtcgaga atcgcgtgat ggaagctgag cgtgaagtgc
    10621 caggtcgcat cgctcctgag gtgtacgcgc caccgcagca gcggggcctg cgccatgtca
    10681 aagtgacggg tcttctcggc ctcgaaccac acgtcgaagg tcttcgcctg ttcggcgtcc
    10741 ggccgatttc cgagatcgta gacctccaat ggcaagggaa ccgaccgatg aacgacctgt
    10801 agcggctcgc tgaattcgct ccagtgaaag gaggtgcgca gtattggatg acgccgcacg
    10861 accacgtcaa ccgctgcccg gaaccgctgc tcatcgaaga gcgaccgaat gcggtagccg
    10921 aagacatcgt ggtacacgcc gctctcggga ttcaactggc tgtggaacag catccctgac
    10981 tggatgcggg ccagcggata cgcgtccacg gcatcggcgg gaagctggac gcgatcagcc
    11041 gccgtcatga gagcaaaggc ttccctgacg ggagcggatc gggggacggg gctcgtccgg
    11101 tccatcatca cgcgcgacag agcgtcaatc gtctgatgtt ggaaaagctc ctgaagcgag
    11161 aagatcaatc cgacctgctt cgctctggct acgatcccga tgctccggat cgagtcgccg
    11221 ccgagctcga agaagttgtc gtagatgccg acccgctcga cgtgcagggc gtccgaccag
    11281 atccccgcca acgcctcttc ttccactgtt cgagggggaa cgtactcatg ccccagcgac
    11341 aggcggacgc cgtcgggcgc aggaagcgcg cggcggtcca gcttgccgtt cggggtgagc
    11401 ggcagggcat cgagcaccac aaatgtcgcc ggcaccatgt agtccggcag cgatgccttc
    11461 aaatggcggc gcagcgtggt cacgtcagga gatccgcctt tgccaacgat gtaggccacc
    11521 agccgctggt ctccaggact gtcctcgcgt gcgaccacga cggccgcttc gatgtacgga
    11581 tgacgaatca ggacggcttc gatctccccg agctcgatcc ggaagccgcg aatcttcacc
    11641 tgctgatcga gccgacccag gaagccgagc tgcccgtcgg ccaggtatcg cgcgccatcg
    11701 ccagtccggt agagccgggc cccctcgcca taggggcttg gcacgaagcg ctgggccgtc
    11761 agctccggcc ggccgtgata tccccgggcc acgcctgcgc cggcgacata gatttcccca
    11821 ggcaggcccg caggcaagag ctcgccgtgc tcgtcgcgaa cgtagacctg aaggtccggg
    11881 atcggccgcc ccgccgggct gtccacgtgc ggcgtatcga gatc
//
LOCUS       DBNGSH010000224         3158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1036508, whole genome shotgun sequence.
ACCESSION   DBNGSH010000224 DBNGSH010000000
VERSION     DBNGSH010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1036508"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="AC16CY_05165"
     CDS             <1..458
                     /locus_tag="AC16CY_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353203.1"
                     /translation="NTPYPHAYVHHPDRLVILWEQDNRYHMVPTDGRELPKDLEPTWM
                     GTSTGRWDGDTLVVTTAGFNGRTWLDTAQHPHSDALRLTVRMTRPDFDHLTWDITMED
                     PKYYVKPIRNARTFVLMEKGQELFEYSCSENNRCEGGKCAEADVQKGAK"
     gene            520..750
                     /locus_tag="AC16CY_05170"
     CDS             520..750
                     /locus_tag="AC16CY_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353204.1"
                     /translation="MRTTVDIPDQMYRRLKGRAAREGRSAKELILRGVEQILAGERRS
                     SGRRVTLPLVRSKRPGSVALDNARIYDVISFP"
     gene            716..1162
                     /locus_tag="AC16CY_05175"
     CDS             716..1162
                     /locus_tag="AC16CY_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HIR3353205.1"
                     /translation="MPASTTSFHFPDVNVWIALTWDGHVHHGTAAQWFNALDEDGRVF
                     FCRFTQLGLLRLLSSDAVMGPDAMTQRAAWLAYDRWQADERVGFLDEPVDLESRFRAL
                     TRLRHPAPKDWADSYLAAFAGASDLTLVTFDGALKSKAERAVLLTA"
     gene            1210..>3158
                     /locus_tag="AC16CY_05180"
     CDS             1210..>3158
                     /locus_tag="AC16CY_05180"
                     /inference="COORDINATES: protein motif:HMM:NF019259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3353206.1"
                     /translation="MSCRSAVMRSVTLAAACFGMALTGIAAPDAAAPRAQTPAAAVAD
                     AEGARGVIARYCTTCHNERAKTGGLALDGLDLTRVADHPDVWEAVVRKVRTGVMPPAG
                     MPRPDRAAASSLASWLEAELDSAARAPRPGRPLLRRLNRAEYANVVRDLLSVDVDVAS
                     LLPADDSAFGFDNIGDLLTVSPALLERYLDAADRVSALAVGDPSTALGSETYHVRGDQ
                     SQSQHLDGLPLGTVGGLTVRHTFPLDAEYEFTLSLFRTNLEAIRGLEHTHQIEISVDG
                     ERIFLGEIGGSAESGQTGTITERSDATDARLKARAPVKAGPRVVAATFIRKMAENTNR
                     LRPFLRSNSGTYDSTGRPHIETLTVTGPFNATGPGDTPSRRRVFTCRPSMPAEEEGCA
                     RRILATLARRAYRRPLTETDLTRLLAFYREGRGKGTFDTGIQLALRRLLASPTFVFRI
                     EDDPAGLAPGTPFAVSNIELASRLSFFLWSSMPDDTLMDVAATGRLRQPEVLEGQVRR
                     MLADPRSDALVENFAGQWLHIRNLRNIAPNSDEFPDFDNDLRDAFRRETELFFGSVMR
                     EDRNVLDLMRADYTFVNERLAKHYGIPNVYGSRFRRVTLADEARRGLLGKGSILLATS
                     HADRTAPVLRGKWIMENLLGSPPPPP"
BASE COUNT          520 a         1079 c         1060 g          499 t
ORIGIN      
        1 acaacacgcc gtacccgcac gcctacgttc accatccgga tcggctcgtg atcctgtggg
       61 agcaggacaa ccggtatcac atggtgccga ccgacggccg tgagctgccg aaggatctcg
      121 agcccacatg gatgggaacg tccaccggcc gctgggacgg cgacacgctg gtcgtgacga
      181 cggcgggatt caacgggcgg acgtggctcg acacggccca gcatccgcac agcgacgcgc
      241 tgcggctgac cgtccgcatg acgcgacccg acttcgatca tctgacgtgg gacatcacga
      301 tggaggatcc gaagtactac gtgaagccga tacggaacgc gcgcacgttc gtgctgatgg
      361 agaagggcca ggagctcttc gagtattcgt gctccgagaa caaccgctgc gagggcggca
      421 agtgcgccga ggcggacgtg cagaagggcg cgaagtagcc gtgcagcggg accgctacga
      481 cgatcaggcc atcaaccata aatatggtag agtacgggtg tgcggacgac cgtcgacata
      541 cccgaccaga tgtaccggcg gctcaagggg cgggccgcgc gcgagggccg ttctgccaaa
      601 gagctcatcc tgcgaggggt cgagcagatc ctggccggtg agcggcgttc atccggtcgt
      661 cgcgtaacgc tcccgctcgt ccgatcgaag cgcccaggct ccgtggcgct cgacaatgcc
      721 cgcatctacg acgtcatttc atttccctga cgtcaacgtc tggatcgcgc tgacctggga
      781 cggccacgtg caccacggca cggcggcgca atggttcaac gccctggacg aagacgggcg
      841 agtgttcttc tgccgcttta cgcagctcgg gttgctccgt ttgctctcgt ccgacgctgt
      901 gatgggtccg gacgcgatga cccaacgtgc ggcatggctc gcctacgatc gctggcaggc
      961 cgacgaacgg gtcggcttcc tcgacgagcc cgtcgacctg gaatcacggt ttcgggcgtt
     1021 gacgcggctc cgacatccgg ccccgaaaga ctgggcggac tcctacctgg cggcctttgc
     1081 tggcgcgtcg gacctcaccc tcgtcacttt cgacggtgcg ttgaagtcga aggcggagcg
     1141 agccgtgctg ctcacggcgt gacggacctc ccgaggaagg ccaggcccga tggtgatata
     1201 agtcgagccg tgtcctgtcg atcggcggtg atgcgaagcg tgacgctcgc cgcggcctgt
     1261 ttcgggatgg cgctgacggg catcgccgcg cccgatgcgg cggcccctcg agcacagacg
     1321 ccggcggccg ccgtggccga tgccgagggc gcgcgcggcg tcatcgcccg ttactgcacg
     1381 acctgccaca acgagcgcgc gaagacgggc ggcctcgcgc tcgacggcct cgatctcacg
     1441 cgggtggccg accacccgga cgtgtgggaa gccgtcgtcc ggaaagtgcg gaccggcgtg
     1501 atgcctccgg ccggcatgcc gcgccccgat cgcgctgccg cgtcgtcgct ggcctcgtgg
     1561 ctcgaagcgg agctcgatag cgccgcccgc gcgccgcggc ccggccgccc gctgctgcgg
     1621 cggctcaatc gagccgagta tgccaacgtc gttcgagacc tgctgtcggt ggacgtggac
     1681 gtcgcctcgc ttctgcccgc ggacgactcc gccttcggct tcgacaacat cggcgatctg
     1741 ctgacggtgt ctcccgcgct cctcgagcgc tacctcgatg cggccgatcg cgtcagcgcc
     1801 ctggcggttg gcgatccgtc gacggcgctc ggatcggaga cctaccacgt gcgcggggat
     1861 cagtcgcaga gccagcacct ggacggcctt ccgctcggaa ccgtcggcgg cctgacggtt
     1921 cgtcacacgt tccctctcga tgccgagtac gagttcacgc tctcgctgtt caggacgaac
     1981 ctcgaagcca tccgcgggct cgagcacacg caccagatcg agatttccgt tgacggcgag
     2041 cgcatcttcc tcggagagat cggcggcagc gcggagtccg ggcagacggg aacgatcacc
     2101 gaacgttccg acgcgacaga cgcgcggctg aaagcccgcg cgccggtgaa ggccgggcct
     2161 cgcgtggtgg ccgcgacgtt catccggaag atggcggaga acacgaatcg cctgcggccg
     2221 tttcttcgaa gcaattccgg cacgtacgat tcgaccggac ggccgcacat cgaaacgctc
     2281 acggtcacgg ggccgttcaa cgcaaccggc ccgggcgaca cgccgagccg gcggcgcgtc
     2341 ttcacgtgcc ggccgtcgat gccggcggaa gaagagggat gtgccaggcg gattctcgcg
     2401 acgctcgcgc gccgcgcgta ccggcggccc ctgaccgaga ccgatctgac tcgtctgctg
     2461 gccttctacc gtgaaggccg aggcaaggga accttcgaca cgggcatcca gctcgctctt
     2521 cgtcggctgc tcgcgagtcc gacgttcgtc ttccggatcg aggacgaccc ggccggcctg
     2581 gcgccgggca caccgttcgc cgtgagcaac atcgagctcg cctcgcggct gtcgttcttc
     2641 ctgtggagca gcatgcccga cgacacgctg atggacgtgg ctgcgaccgg ccgcctgcgt
     2701 cagcccgagg tgctcgaggg ccaggtgcgc cgcatgctcg ccgatccgcg atcggacgcg
     2761 ttggtcgaga acttcgcggg acagtggctc cacatccgca acctccggaa catcgcgccg
     2821 aactccgacg agttcccgga tttcgacaac gaccttcgcg acgcgttccg ccgcgagacc
     2881 gaactcttct tcggcagcgt catgcgcgag gatcgcaacg tcctcgacct gatgcgggcc
     2941 gactacacgt tcgtcaacga acggctggcg aagcactacg gcattcccaa cgtgtacggc
     3001 agccgtttcc gccgcgtgac gctggccgac gaggcgcggc gcgggctgct cggcaagggc
     3061 agcattctgc tggccacctc gcatgccgat cggaccgcgc cggtcctgcg gggcaagtgg
     3121 atcatggaga acctgctcgg ctcgccgccg ccaccgcc
//
LOCUS       DBNGSH010000225         2192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3922988, whole genome shotgun sequence.
ACCESSION   DBNGSH010000225 DBNGSH010000000
VERSION     DBNGSH010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3922988"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="AC16CY_05185"
     CDS             complement(<1..757)
                     /locus_tag="AC16CY_05185"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR3353207.1"
                     /translation="MRILTAAQMREADRFTIEEIGIPSLVLMENAGRQVVSAIESAFE
                     SRLNGRVAVLCGRGNNGGDGFVVARTLLQRGIDTSVFVIGALAEVRGDARTNLEILGR
                     LGVTVVEVADEQSWELHFSEISRCTLIVDAIFGTGLKTAVTGTIETIIADVNASDIPI
                     VSVDLPSGLSADTPHLIGACFDASVTVALGAPKVPLVLPPGEAYAGDVVIADIGIPAE
                     VINGLEGPHLELLTPEQLRGLIGPRAVDSHKGDF"
     gene            complement(835..>2192)
                     /gene="gcvPB"
                     /locus_tag="AC16CY_05190"
     CDS             complement(835..>2192)
                     /gene="gcvPB"
                     /locus_tag="AC16CY_05190"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR3353208.1"
                     /translation="LDTSMIPLGSCTMKLNPATAMRPVTWPAFGRLHPFVPEDQAGGY
                     AEVFRELESALCEITGFARVSLQPNSGAQGELTGLMVIRAYHRDRGEADRTIVLIPAS
                     AHGTNAASAAMAGMQVVVVRSTDEGSVDVEDLRQKAAQHRARLACLMVTYPSTHGVFE
                     DDIQALCEIVHEYGGQVYMDGANMNAQVGLTSPASIGADVCHLNLHKTFAIPHGGGGP
                     GMGPIGVAAHLSPYLPGHPLVPTGGERAIHAVSAAPWGSASILLISHAYIRMLGPDGL
                     MDATRHAILNANYLKARLEPHYPVLYARGNGRVAHELIFDMRAFKPHVDEMDVAKRLI
                     DYGFHAPTVSFPVAGTLMVEPTESEDKSELDRFCDAMIAIRHEIEAVMSGKAHADDNV
                     LKNAPHTAAAATAGEWTHPYSREQAVFPLPFVRERKFWPPVGRVDNVFGDRNVVCRLC
                     V"
BASE COUNT          357 a          759 c          715 g          361 t
ORIGIN      
        1 cgaagtcgcc cttgtgcgaa tcgacggcgc gcggcccgat caggcccctc agctgctccg
       61 gcgtcagcag ctccagatgc ggcccttcga gcccgttgat gacctccgcg ggaatgccga
      121 tgtccgcaat caccacgtcg cctgcgtagg cctcgcctgg tggaaggacg agcggcacct
      181 tgggcgctcc aagggcgacc gtcaccgacg cgtcgaagca cgcgccgatg agatgcggcg
      241 tgtcggcgct caggccgctg ggcagatcga cggacacgat cgggatgtcc gacgcgttca
      301 cgtccgcgat gatcgtctcg atcgtgccgg tgacggccgt cttgaggccc gtgccgaaga
      361 tggcgtccac gatcaacgtg caccgggaga tctcggagaa atgaagctcc cagctctgct
      421 cgtccgcgac ttcgacgacc gtgacgccaa gccggccgag gatctcgagg ttggtccgcg
      481 cgtcgccgcg gacctcagcc agtgcgccga tcacgaagac cgacgtgtcg atgccgcgct
      541 gcagcagcgt ccgcgccacg acgaagccgt cgcccccgtt gttgccgcgg ccgcagagca
      601 ccgcgacccg gccgttcagc ctggattcga acgccgactc gatcgccgag acgacctgcc
      661 gtcccgcgtt ctccatcagc acgagggacg gaatgccgat ctcttcgatc gtgaagcggt
      721 cggcctcgcg catctgcgcg gcggtcagga tccgcatcgg cgattgcccg attccattcc
      781 ctgaggatac gccatgtcac acggaagctg ggcgagctgc tcgaacgggt cgcctcacac
      841 gcatagccgg cacacgacgt tacggtcgcc aaacacgttg tcaacgcgcc ccacgggcgg
      901 ccagaacttc ctctctctca cgaacggcag cgggaacacc gcctgctccc gcgagtaggg
      961 atgggtccac tcccccgccg tcgccgccgc ggccgtgtga ggggcgttct tcagcacgtt
     1021 gtcgtccgcg tgggccttcc ccgacatgac cgcctcgatc tcgtgtcgaa tggcgatcat
     1081 cgcgtcgcag aagcgatcga gctcggattt gtcttcgctc tccgtcggtt cgaccatgag
     1141 cgtgccggca acgggaaacg acacggtcgg tgcatgaaag ccgtagtcga tcagccgctt
     1201 ggcgacgtcc atctcgtcca cgtgcggctt gaaggcgcgc atgtcgaaga tcagctcgtg
     1261 cgcgacgcgg ccgttgcccc tggcgtacag caccgggtag tgaggctcga gccgcgcttt
     1321 gaggtaattc gcgttcagga tggcgtgacg cgtcgcgtcc atcaggccgt cggggccgag
     1381 catccggatg taggcgtggg agatcagcag gatgctcgca ctcccccacg gcgccgcgga
     1441 cacggcgtga atcgcgcgct cgccgcccgt cgggacgagc gggtgcccgg gcagatatgg
     1501 cgacagatgt gccgccacgc cgatgggccc catccctggt ccgccgccgc cgtgagggat
     1561 ggcgaacgtc ttgtgcaggt tcaggtgaca cacgtccgcg ccgatcgacg ccgggctcgt
     1621 caggccgacc tgcgcgttca tgttcgcgcc gtccatgtac acctgaccgc catactcgtg
     1681 aacgatctcg cagagtgcct ggatgtcgtc ctcgaacacg ccgtgcgtgg acgggtaggt
     1741 caccatcagg caggccagcc gcgcgcggtg ctgcgccgcc ttctgccgga gatcctctac
     1801 gtccacgctg ccctcgtctg tgctgcgcac gacgaccact tgcattcccg ccatcgccgc
     1861 gctcgcggcg ttggtgccgt gagccgacgc cggaatgagc acaatcgtgc gatcggcctc
     1921 gccccgatcg cgatggtagg cgcggatgac catcagtccc gtcagctcgc cctgcgcgcc
     1981 cgagttcggc tgcagcgaca cgcgcgcaaa cccggtgatc tcgcacaacg cggactcgag
     2041 ctcgcggaat acttcggcgt agccaccagc ttgatcctcg ggaacgaacg gatgcaggcg
     2101 gccgaaggcc ggccacgtca ccggccgcat cgccgtcgcg gggttgagct tcatggtgca
     2161 ggagccgaga ggaatcatcg acgtgtcgag cc
//
LOCUS       DBNGSH010000226         1641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_18083, whole genome shotgun sequence.
ACCESSION   DBNGSH010000226 DBNGSH010000000
VERSION     DBNGSH010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_18083"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="AC16CY_05195"
     CDS             complement(<1..887)
                     /locus_tag="AC16CY_05195"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3353209.1"
                     /translation="MADIFLSYSRSDLPRARPFEQALEACGWSVFWDRELLPGEGFRR
                     AIEHELKQARCVIVLWSKTSIDSEWVIDEAESGKQNGRLVSVRIDDVEMPLGFRQLQA
                     ATLVDWRGETEHPEFQLLTRRLRALIPSGADLPPAEARENEPREKEPRESEPREHEPP
                     PESFLARLAVRHPSAEPTMLLAAVFLVNYIQTSVDGAITPRRLGVEAGYPIADAFRWF
                     ERYLSFELHDTTSAIAYYGYSASYFFIFPVLCLSVVWALARRRDPRPYQAVSLAVAIN
                     YLVSLPFFIFFPVPERWSSP"
     gene            complement(892..>1641)
                     /locus_tag="AC16CY_05200"
     CDS             complement(892..>1641)
                     /locus_tag="AC16CY_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353210.1"
                     /translation="GDLLDAARLAIRLAGTELVDKSQYFDYYKKSDIRWELASIHDGA
                     VRLTVRYENFRLDNSEVTQQFDVSFLTPADLSRRLVSLIHSRLHRIRPLWLYNDTAPT
                     VLRDVDFDIAERVPFQEIQWYEPQRNDYRLEGESHVANVEKEETFEIQLGKAEFPIRP
                     DNRGLDFEPMGRRGFRVLLMRVTPERPLFRALTVAFVVLLVAAAAALVWDGWRWRASQ
                     LARVVRERGFSRPEDVVPSHATAGDVAMNSR"
BASE COUNT          294 a          544 c          527 g          276 t
ORIGIN      
        1 ggggacgacc agcgctccgg caccgggaag aagatgaaga acggcaggct gaccaggtag
       61 ttgatggcca cggcaaggct gacggcctgg tacgggcgcg ggtccctcct gcgggcgaga
      121 gcccagacga cactcaggca caacacggga aagatgaaga agtacgacgc cgagtatccg
      181 tagtacgcga tggcgctggt cgtgtcgtgc agctcgaagc tgagatatcg ctcgaaccac
      241 cggaaggcgt cggcaatcgg atagccggcc tcgacgccga gacggcgggg cgtgatcgcc
      301 ccgtccacgc tcgtttgaat gtagttgacc aggaacacgg cggctaggag catcgtcggt
      361 tccgctgatg gatggcgcac ggcgaggcga gcgaggaacg actcgggcgg cggctcgtgt
      421 tcccgaggct cgctctctcg gggctctttc tctcggggct cgttctctcg agcctccgcc
      481 ggcggaaggt ccgctcctga tggaatgagg gcacgcaagc ggcgcgtcag aagctggaac
      541 tccgggtgct cggtctctcc ccgccagtcc accagcgtgg cggcctgcaa ctgcctgaag
      601 cccagcggca tctcgacatc gtctatcctg accgacacca gccggccgtt ctgcttgccg
      661 gactcggcct cgtcgatcac ccactcgctg tcgatcgagg tcttcgacca cagcacgatg
      721 acgcagcgcg cctgcttgag ctcgtgctcg atggcgcgac ggaatccctc cccgggcagc
      781 agctcgcggt cccagaagac actccagccg cacgcttcga gcgcctgctc gaatggccgc
      841 gcccttggca gatccgatcg cgagtagctg aggaagatgt ccgccatcga tctaccggct
      901 gttcatggcg acgtcgccgg ccgtggcgtg agacggcacg acgtcctccg gcctcgaaaa
      961 cccgcgttcg cgaacgactc gcgcaagctg actcgcccgc cagcgccacc cgtcccacac
     1021 gagggccgcc gctgcggcga ccagcaacac cacgaacgcg acggtgagcg cccggaacag
     1081 cggccgttcg ggcgtcactc gcatgagcag cacgcggaag ccccgtctcc ccatcggttc
     1141 gaagtccagg cccctgttgt ccggacgtat cgggaattcg gccttcccca gctgaatttc
     1201 gaacgtctcc tctttctcga cgttcgcgac atgcgactcg ccttcgagcc gatagtcgtt
     1261 ccgctgcggc tcgtaccact ggatttcctg aaacggaaca cgctcggcga tgtcgaaatc
     1321 cacatcgcgc aacacggtcg gcgcggtgtc gttgtagagc cagaggggcc gaatccggtg
     1381 caggcgtgaa tgaatgagcg acaccaggcg ccggctcaga tcggcaggcg tcaggaagct
     1441 gacgtcgaac tgctgcgtga cctccgagtt gtccagacgg aagttctcgt accggacggt
     1501 gagccggacc gcgccgtcgt gaatgctcgc cagctcccat ctgatgtcgc tcttcttgta
     1561 gtagtcgaag tactgactct tgtcgacgag ctcggttccc gcgagccgga tcgcgaggcg
     1621 cgccgcgtcg agcaggtctc c
//
LOCUS       DBNGSH010000227         3304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3413652, whole genome shotgun sequence.
ACCESSION   DBNGSH010000227 DBNGSH010000000
VERSION     DBNGSH010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3413652"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /gene="moeB"
                     /locus_tag="AC16CY_05205"
     CDS             <1..1140
                     /gene="moeB"
                     /locus_tag="AC16CY_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR3353211.1"
                     /translation="EIKRYSRHLIMPEMGMDGQRKLKASSVLCIGAGGLGSPAAMYLA
                     AAGIGRLGIVDFDVVDFSNLQRQLLHTTNDVGRSKLESAKSKVVALNPHVQVDTYEEA
                     LSSENAMRLFKGYDVILDGTDNFPTRYLVNDACVLSGIPNAYGSIFRFEGQASVFATK
                     DGPCYRCLYPEPPPPGLVPSCAEGGVLGVLPGIIGVIQATESIKLVTGIGEPLIGRFL
                     IYDALRMRFRELKLKKDPECPVCGTSPTVTKLIDYEQFCGVGPAAQTTEVPGGAVNEG
                     EITAVELKKRLNAGDTLLILDVREPTEYQINRIEGSTLIPLGELPRRYQELPRDREIV
                     TQCKIGARSAKARDFLKSVGFTNVRNLRGGILDWIDKVDPSLPRY"
     gene            1274..1768
                     /locus_tag="AC16CY_05210"
     CDS             1274..1768
                     /locus_tag="AC16CY_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3353212.1"
                     /translation="MLRLSKKADYALIALNHLAHRQDTRASASARDIAERYDIPIELM
                     AKVLQRLVRTGLLVSTQGTRGGYALSRPSRSISVADVIQAIDGPFTVTACSTEKHDCE
                     QYSKCNVRAPLWQIREKILAALGTVSLAEMSSESESPAAELAVVRRSAMTGSAGLGPR
                     QGRT"
     gene            1765..2979
                     /locus_tag="AC16CY_05215"
     CDS             1765..2979
                     /locus_tag="AC16CY_05215"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HIR3353213.1"
                     /translation="MKLPIYMDYHATTPVDPRVVETMLPFFSQHFGNAASRNHSFGWE
                     AEEAVENARKQVADIVGASAKEIIFTSGATESNNLAIKGVAEMYREKGNHIITCVTEH
                     KAVIDTCKKLEKQGARVTYLPVQKDGRISLDDLRAAITDKTILITIMTANNEIGVLQP
                     ITEIGAIAKEKGILFHTDAVQAVGKVPVSVNDLKVDLLSLSAHKMYGPKGVGALYVRR
                     RNPRVLLSEQISGGGHERGMRSGTLNVPGIVGLGKAAELCRTEMAEESGRLRKLRDRL
                     NEGLHRGLDELYVNGSIEHRLPHSLNISFAYVEGESLLMGINDVAVSSGSACTSASLE
                     PSYVLKALGAGDDLAHSSIRFGLGRWTTEEEVDYVVERLTNVVRRLREMSPLYEMVKE
                     GVDLSKMQWATH"
     gene            3027..>3304
                     /locus_tag="AC16CY_05220"
     CDS             3027..>3304
                     /locus_tag="AC16CY_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR3353214.1"
                     /translation="MAYSNKVIDHYENPRNVGSLPKDSTDVGTGLVGAPECGDVMKLQ
                     VMVNPDTGIIDDAKFKTFGCGSAIASSSLATEWLKGKTVDQALAIKNT"
BASE COUNT          653 a         1010 c         1123 g          518 t
ORIGIN      
        1 gagatcaagc gctacagccg gcatctgatc atgccggaga tggggatgga cggtcagcgc
       61 aagctgaagg cgtcgagcgt gctctgcatc ggcgccggcg gtctgggctc ccccgcggcg
      121 atgtatcttg ccgccgcggg catcggacgt ctcgggatcg tcgatttcga cgtcgtggac
      181 ttcagcaacc tccagcgtca gttgctgcac acgaccaacg acgtcggccg gtcgaagctc
      241 gagtcggcga agagcaaggt cgtggccctg aatccgcacg tgcaagtgga cacctatgaa
      301 gaagcgctct cctccgagaa cgcgatgcgc ctcttcaagg gctacgacgt gatcctcgac
      361 gggaccgaca acttcccgac gcggtacctg gtgaacgacg cctgcgtgct ctcgggcatt
      421 ccgaacgcgt acggcagcat cttcaggttc gaagggcagg cctctgtctt cgccaccaag
      481 gacgggccgt gctatcgctg cctctatccg gagccgccgc cgccaggcct ggtcccaagc
      541 tgtgccgaag gcggtgtgct tggcgtgctg ccgggaatca tcggcgtgat tcaggccacc
      601 gagagcatca agctggtcac gggaatcggc gagccgctca tcggacgctt cctcatctac
      661 gacgcgctgc ggatgcgctt ccgcgagctg aagctgaaaa aggatcccga gtgcccggtg
      721 tgcggcacga gtcccacggt gacaaagctg atcgactacg aacagttctg cggtgtgggc
      781 ccggccgccc agacgacaga ggttcctgga ggagccgtga acgagggcga gatcaccgcg
      841 gtggagttga agaagaggct gaacgccggg gacacgctgc tgatcctcga cgttcgcgag
      901 ccgaccgagt atcagatcaa ccgcatcgaa gggtccacgc tgattccgct cggcgagctg
      961 ccgcggcgat accaggagct gccccgcgat cgcgagatcg tcacgcagtg caagatcggc
     1021 gctcgcagcg ccaaggcgag ggacttcctg aagtcggtcg ggtttaccaa cgtcaggaac
     1081 ctccgcggcg gcatcctcga ctggatcgac aaggtggatc cctcgctgcc gcggtactag
     1141 gcctcagggc ccaggcccca gggcggtctc agggcctcag gcctggccca gccctgagtc
     1201 ctgggccctg attcctgagg cctttgacgt agtccagaat accgactaga ctagtcggag
     1261 ttagccaagg cacatgttaa ggctgtcgaa gaaggccgat tacgcgctga ttgcgttgaa
     1321 ccacctggcc cacaggcagg acaccagggc gtcggcgagc gcccgtgaca tcgccgaacg
     1381 ctacgacatc ccgatcgagt tgatggcgaa ggttcttcag cgtctggtgc ggacgggcct
     1441 gctcgtctcg acgcagggga cgcgcggcgg gtacgcgctc agccggccgt cgcgatcgat
     1501 cagcgtggcc gacgtgattc aggcgatcga cggcccgttc acggtgacgg cctgctcgac
     1561 ggagaagcac gactgcgagc agtacagcaa gtgcaacgtg cgggcgccgc tgtggcagat
     1621 ccgcgaaaag atcctggcgg cgctcggcac ggtgagtctc gcggagatgt cctcggagag
     1681 cgaatcgccg gcggccgaac tggccgtcgt acgccggagc gcaatgactg gaagtgcggg
     1741 cctcggtccg cggcaggggc gaacatgaag ctgccgatct acatggacta tcacgccacg
     1801 accccggttg acccgagggt ggtggagacg atgctgccct ttttcagcca gcatttcggc
     1861 aacgcggcca gccggaacca ctcgttcggc tgggaggccg aggaagcggt cgagaacgcg
     1921 cgtaagcagg tcgccgacat cgtcggggcg agcgccaagg agatcatctt cacgagcggc
     1981 gcgaccgagt cgaacaacct ggccatcaag ggcgtcgcgg agatgtaccg cgagaagggc
     2041 aaccacatca tcacgtgcgt caccgagcac aaggccgtca tcgacacgtg caagaagctc
     2101 gagaagcagg gcgcgcgggt gacctacctt ccggtccaga aggacgggcg catcagcctc
     2161 gacgatcttc gcgcggcgat taccgacaag acgatcctca tcaccatcat gaccgccaac
     2221 aacgagatcg gcgtgctgca gccgatcacc gagatcggcg cgatcgccaa ggagaagggg
     2281 atcctcttcc acaccgacgc cgtccaggcc gtcggcaagg taccggtcag cgtgaacgac
     2341 ctgaaagtgg atctgctgtc gctgagcgcc cacaagatgt acgggcccaa gggggtcggg
     2401 gcgctctacg tgcgtcgccg caatccgcgc gtgctgctgt cggagcagat cagcggcggc
     2461 ggccacgagc gcggcatgcg ctcgggcacg ctcaacgtcc ccggcatcgt cgggctgggc
     2521 aaggccgccg agctgtgcag gacggagatg gcggaggagt ccggtcgctt gcgcaagctg
     2581 cgagaccggc tcaacgaggg gctgcaccgg ggcctcgacg agctgtacgt caacggctcg
     2641 atagagcatc gcctgccgca cagcctgaat atcagcttcg cgtacgtgga aggcgagtcg
     2701 ctgttgatgg gcatcaacga cgtggcggtg tcgtcggggt cggcctgcac gtcggcgagc
     2761 ctcgagccgt cctacgtgct caaggcgctc ggggccggcg acgacctggc gcactcctcg
     2821 attcgattcg gcctcggccg ctggacgact gaagaagagg tcgattacgt ggttgaaaga
     2881 ctgaccaacg tggttcggcg gctgcgtgag atgtcgccgt tgtacgagat ggtgaaagaa
     2941 ggcgtggacc tgtcgaagat gcagtgggcc acgcattgag aaggccccag gaatcagggc
     3001 ccaggcctca gtgaggagca gatgaaatgg cgtattcaaa caaggtgatc gatcactacg
     3061 agaacccgcg caacgtcggc tcgcttccga aggacagcac cgacgtgggg acggggctcg
     3121 tcggcgcgcc ggagtgcggc gacgtcatga agctccaggt catggtgaac ccggacacgg
     3181 gcatcatcga cgatgcgaag ttcaagacgt tcgggtgcgg atccgcgatc gccagctcca
     3241 gcctggccac cgagtggctc aaggggaaga cggtcgatca ggcgctcgcg atcaagaaca
     3301 ccga
//
LOCUS       DBNGSH010000228         4719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2394997, whole genome shotgun sequence.
ACCESSION   DBNGSH010000228 DBNGSH010000000
VERSION     DBNGSH010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2394997"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..1446)
                     /locus_tag="AC16CY_05225"
     CDS             complement(379..1446)
                     /locus_tag="AC16CY_05225"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HIR3353215.1"
                     /translation="MTRRRTRVLVIAVAAAAFACVAPAQQRKPNVVILATGGTIAGAG
                     ATGTQSAYKSGAVGIDTMVAAVPGIGDLATIRGEQISNVGSQDMSFAILLSVAKRINE
                     LARAADVDGFVITHGTDTLEESAFFLNLTVKTDKPVVMVGSMRPSTAVSADGPLNLYN
                     AVGVAADRAARGRGVLVVMNDWIHAAHSLTKTSTTAVQTFMSPVRGLVGVAAYGKNDF
                     YSTPPWKHTTQSEFDISQVSAVPRVDILFAAIDMPPDLIDASAASGARGIVVAGVGNG
                     NMNKAAIDAAARAVKKGVVVVRSSRVATGNVGRNVEINDDELGFIASDELNPQKSRVL
                     LSLALLTKRTPAQIQTLFQTY"
     gene            complement(1518..3068)
                     /locus_tag="AC16CY_05230"
     CDS             complement(1518..3068)
                     /locus_tag="AC16CY_05230"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR3353216.1"
                     /translation="MRRRSGLPIAMTLALAATTGACGPDATSPPASQPQQAPAATTPA
                     GATRTERDLLGPKEVPANAYYGVQTARALENFRISGIPISHYPGFVEAWAIVKLAAAR
                     ANTDVGAMNKETLAAIEKAADAVRKGEYHDQFQVDWYQGGAGTSTNMNANEVLANVAL
                     ELTGHKKGEYTIVEPHDHLNLSQSTNDSYPTALKVAFILRNDRLTAELENLVSAFRAK
                     GDEFIDVVKMGRTEMQDAVPMTVGQEFHAFAAALEDEIALLRDAEKPLYVVNMGATAI
                     GTGINVPKGYAARTATHLATLTGKPIVPARDMIAATWDQQGFVVYSSALKSLAIKLSK
                     IAGDLILLASGPRAGLAEINLPALQPGSSIMPGKVNPVIPELVNLVAFRVMGNDFSVT
                     LAAHSGQLQLNAYEPLAGLAVMESQHLLYNTSIALRTSCIDGITVNRKVLDRYLETTV
                     GIVTALNPVLGYDKATELANEAYKSDKGILEIVRERKLLTDKQIAELLDPAKLTMLDK
                     SIYPKPKK"
     gene            3315..>4719
                     /locus_tag="AC16CY_05235"
     CDS             3315..>4719
                     /locus_tag="AC16CY_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3353217.1"
                     /translation="MRGLMMDRPLLISSLLEHAAEVFSSVEIVTRTVEGPIHRYTWRD
                     VRRRSAQLAQALQALTLHDGDAVATIAWNTHRHLELYYGVSGMGAVVHTVNPRLHPAQ
                     LVYVLNHAQDRVLFVDLTFVPLVEAVWSQLTSVRHLVIMTDRAHMPACKVPGALCYEE
                     LIARQNGDYQWPTFPEDTAAAICYTSGTTGNPKGVVYSHRSSVLHAFGISIPGVIPVG
                     PGEVLLPVVPMFHVNAWGVPYAAAMCGYKLVMPGPRLDGAGLTELMNAEGVTCYWGVP
                     TVHMGLLAHWRASGQSVQSLRLVATGGSAPTSSLIEGFRSRGIEVVHGWGMTETSPLA
                     TISRITAAESGLDDVAQVARLMRQGRKLFGVEIRIVGEDGRALPRDGKAFGELQVRSP
                     WVCSAYLGDAPDSALDGEGWFSTGDVAVIHPDGTMQITDRKKDLIKSGGEWISSIDLE
                     NAAARHPEIAIAAVIGVP"
BASE COUNT          704 a         1561 c         1588 g          866 t
ORIGIN      
        1 gccatcgtcc cgagcgcgat cccgccgagc cgcgcgccga cgaggatgca ggcgagaaag
       61 acgaggagct ccagccagat catgtcggcc agagcctaca cgatagcgac ctggcggccg
      121 gacccggcct caggatgtct ggggtggctg ctgggacctg agcttcagtt cgatttggcc
      181 gcgcatgatg gagcaccaat ccttgtgctc cgccactggc atcccgcact cgatgcagac
      241 ccgcacccgc ggcttcggtg gttcggcctt gcccagcaat cgtttcagaa atgacatgcg
      301 ggaccccccg tgcctcgctg acgaccgtgt atacgtttga cgaggcacga gggttcacgg
      361 ttggcgctcc gctggcgctc agtaggtctg gaacagggtc tggatctggg ccggcgtgcg
      421 cttggtcagc agggccagcg acagcagcac gcgcgacttc tgggggttga gctcgtcgga
      481 cgcgatgaag cccagttcgt cgtcgttgat ttccacgttg cgcccgacgt tgccggtggc
      541 gacgcgggag ctgcgcacga ccacgacgcc cttcttcacc gcccgggccg ccgcgtcaat
      601 ggccgccttg ttcatgttgc cgttgccgac gccggcgacg acgatgcccc gggcgccgct
      661 ggccgccgag gcgtcgatca ggtccggcgg catgtcgatg gccgcgaaga ggatgtccac
      721 ccgcggcacc gcgctgacct gcgagatgtc gaactcgctc tgggtggtgt gcttccacgg
      781 cggcgtggaa tagaagtcgt tcttgccgta cgccgccacg ccaacgaggc cgcgaaccgg
      841 cgacatgaac gtctgcaccg ccgtcgtgct cgtcttggtc agcgagtgtg ccgcgtgaat
      901 ccagtcgttc atcacgacga gcacgccgcg tccccgcgct gcccgatcgg cggccacccc
      961 gaccgcgttg tagaggttca gcgggccgtc cgcgctgacg gcggtggacg ggcgcatcga
     1021 gcccaccatc accaccggct tgtcggtctt caccgtgagg ttcaggaaga acgccgactc
     1081 ttcgagcgta tcggtgccgt gggtgatgac gaagccgtcc acgtcggccg ccctcgccag
     1141 ctcgttgatc cgcttggcga cgctgaggag aatcgcgaag ctcatgtcct gcgagccgac
     1201 gttcgagatc tgctcgcccc tgatggtggc gaggtcgccg atcccgggca cagccgcgac
     1261 catcgtgtcg atgcccaccg cgcccgactt gtaggcggac tgcgtccccg tcgcgccggc
     1321 gcccgcgatc gtcccgcccg tggcgaggat gacgacgttc ggcttcctct gctgcgccgg
     1381 ggcaacgcag gcgaacgccg cggcggcgac cgcgatcacc agtacgcgcg tccttctccg
     1441 ggtcatcatg gtgtgctcct cttcgaggtc tgcagggccg cgtggggccg cattacttct
     1501 tcgattttgg gcagatgtta cttcttcggt tttgggtaga tgctcttgtc cagcatcgtc
     1561 agcttcgcgg gatcgagcag ctcggcgatc tgcttgtccg tcagcagctt tctctcgcga
     1621 acgatctcga ggatgccctt gtcgcttttg taggcttcgt tggccagctc cgtcgccttg
     1681 tcgtagccga gcaccggatt cagcgccgtg acgatgccga ccgtggtttc gaggtagcga
     1741 tcgagcactt tccggttgac ggtgatgcca tcgatgcacg acgtgcgcag cgcgatcgat
     1801 gtgttgtaca ggagatgctg cgactccatc acggccaggc ccgccagcgg ctcgtaggcg
     1861 ttcagctgga gctggcccga gtgggcggcg agcgtgacgc tgaagtcgtt gcccatcacc
     1921 cggaaagcga ccaggttgac gagctcggga atcaccgggt tgaccttgcc cggcatgatc
     1981 gacgagccgg gctgcagcgc cggcaggttg atctcggcca ggccggcgcg ggggcccgag
     2041 gcgagcagga tcagatcgcc ggcgatcttc gacagcttga tggccaggct cttcagcgcc
     2101 gaggagtaga cgacgaagcc ctgctgatcc caggtggccg cgatcatgtc ccgcgccggg
     2161 acgatgggct tgcccgtgag cgtggccagg tgcgtcgccg ttcgggccgc gtagcccttc
     2221 ggcacgttga tgcccgtgcc gatcgcggtg gcgcccatgt tcacgacgta gagcggtttc
     2281 tcggcgtcgc gcagcagcgc gatctcgtct tccagcgcgg cggcgaaggc gtggaattcc
     2341 tggccgaccg tcatcggcac ggcgtcctgc atctcggtgc ggcccatctt gacgacgtcg
     2401 atgaactcgt cgcccttcgc ccggaaggct gagaccaggt tctcgagctc cgccgtcagc
     2461 ctgtcgttgc gcaggatgaa ggcgactttg agcgccgtgg gatacgagtc gttcgtcgac
     2521 tgcgagaggt tcaggtgatc gtgcggctcc acgatcgtgt actcgccctt cttgtgcccc
     2581 gtgagctcga gcgcgacgtt ggcgagcacc tcgttggcgt tcatgttggt ggaggtgccg
     2641 gcgccgccct ggtaccagtc cacctggaac tggtcgtggt actcgccttt gcgcaccgcg
     2701 tcggccgcct tctcgatcgc cgccagcgtc tccttgttca tggcgccgac gtcggtgttc
     2761 gcgcgtgccg ccgccagttt cacgatcgcc cacgcttcga cgaaccccgg atagtggctg
     2821 atggggatgc ccgagatccg gaagttctcg agcgcccggg ccgtctggac gccgtagtac
     2881 gcatttgccg ggacctcttt cgggcccagc aggtcgcgtt cggtgcgggt ggcgcccgct
     2941 ggcgtggtgg cggccggcgc ttgttgtggc tgcgatgccg ggggcgaggt cgcgtcgggc
     3001 ccgcacgcgc ccgttgtcgc cgccagggcg agcgtcattg cgatcggcag gccgcttcgt
     3061 cgtctcatcg tgctgattct catggtcatg caccggttct tgtagggagt tgcggcagca
     3121 atcgtaccag cgtgccattt cgaggcggaa gcgcaaatcg agcgattgcg aaccgcggag
     3181 cgcgcggccc tccgggaatc cgtagggcct ccgtcccggc ggagtgccaa ggagagagca
     3241 gggcagtccc tcaggtagac ttcacgccat tcgatcagac gcctattcga gggctcgccg
     3301 cccggaggtt cgctatgcgt ggactcatga tggaccgacc gctcctcatc tcgtctctgc
     3361 tggagcacgc cgccgaagtg ttctcgtcag tcgagatcgt gacccgcacc gtcgagggtc
     3421 cgatccaccg gtacacctgg agggacgtcc gcaggaggtc ggcgcagctg gcccaggcgc
     3481 tccaggccct gacgctgcac gacggcgacg cggtggccac catcgcgtgg aacacgcacc
     3541 ggcatctcga gctgtactac ggcgtgtcgg gtatgggcgc cgtcgtccat accgtgaatc
     3601 cgcgcctgca ccccgcgcag ctcgtgtacg tcctcaacca cgctcaggac cgcgttctct
     3661 tcgtggacct gaccttcgtc cctctcgtcg aagcggtctg gagccagctg acgtcggtgc
     3721 gccacctcgt gatcatgacc gaccgcgcgc acatgccggc gtgcaaggtc cccggggcgc
     3781 tgtgctacga agagctcatc gcgcgccaga acggagacta ccaatggccc accttccccg
     3841 aggacaccgc ggctgcgatt tgctacacgt ctgggaccac ggggaacccg aagggcgtgg
     3901 tctactctca tcgctcgtcc gtgctgcacg cgtttggcat ttcgattccc ggcgtcatcc
     3961 cggtcgggcc tggcgaggtg ctgctgcccg tcgttcccat gttccacgtc aacgcctggg
     4021 gagtccccta cgcagcggcc atgtgcgggt acaagctcgt gatgcccggc ccgcgactcg
     4081 atggcgccgg ccttaccgag ctgatgaacg cggaaggcgt cacctgttac tggggcgtac
     4141 ccaccgtgca catgggcctg ctcgcccact ggcgggcgtc cggccagagc gttcaatcgc
     4201 tgcgcctggt cgcgacgggc gggtccgccc ccacgtcctc gttgatcgaa ggattccggt
     4261 cccgagggat cgaggtcgtt cacggctggg ggatgacgga aaccagcccg ctggccacga
     4321 tcagccgtat caccgctgcc gagtccggcc tcgacgatgt ggcacaggtc gcgcgcctca
     4381 tgcggcaggg gaggaagctg ttcggcgttg agatacgaat cgtgggcgag gatggccggg
     4441 cactgccgcg cgacggaaag gcattcggag agctgcaggt gcgcagcccc tgggtctgct
     4501 cggcgtatct cggcgacgcg cccgacagcg ccctcgacgg agaaggctgg ttctcgaccg
     4561 gcgacgtcgc ggtcatccac ccagacggga caatgcagat caccgaccgg aagaaggacc
     4621 tcatcaagtc ggggggcgag tggatcagct ccatcgatct cgagaacgca gcggcccgcc
     4681 accccgagat cgccatcgcc gccgtcatcg gcgtgccgc
//
LOCUS       DBNGSH010000229         2723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3923165, whole genome shotgun sequence.
ACCESSION   DBNGSH010000229 DBNGSH010000000
VERSION     DBNGSH010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3923165"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="AC16CY_05240"
     CDS             <1..645
                     /locus_tag="AC16CY_05240"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3353218.1"
                     /translation="ARPFSAACAELPMISMLLPERRMLANSFKDGRPLGRVNDVMADG
                     KGGAFFTSGGAFHVSAEGVVTTVEDQNIRSNGIMLSPDGKTLYVTNNDVVLAFDVQPD
                     GSTRNRRNFGGLNGDGGGDGMAVDGTGRLYVTGNLGVHVLSPEGKYVGLIPTPRRPIT
                     LAFSGPDKKTLYVPQMGAVGPDGKAWATPEGIRNTAMTIYRISMLAEGFKGRPK"
     gene            660..1268
                     /locus_tag="AC16CY_05245"
     CDS             660..1268
                     /locus_tag="AC16CY_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013340.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR3353219.1"
                     /translation="MRTRLLVSVLAVMIATALGAELSGQQQGDRGGGAGRGGGRGRGL
                     APLLMETDAFPDGGVVPPKYAGRGGVQPGFKFSNVPEGTVSFAIIFHDIDVAIGGNTD
                     DVLHWIVWNIPAAAGGIPEGKLPDGSVQGANITKQNAYFGPGAPAGPRYHHYVFELYA
                     LNAMLDLPPTSGRVDLLKAMEGKVVAKSAYVGRFRGEAPPAK"
     gene            1295..2359
                     /locus_tag="AC16CY_05250"
     CDS             1295..2359
                     /locus_tag="AC16CY_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3353220.1"
                     /translation="MKALTLAGLIVAACSLAAVAQTPAPCAASGSTQFVCGVQAPEDL
                     VVLPGAQWVVASAYSGSGGVYLVRVSDRTAMVAYPGASPRERLDAKTYNSCPGPPDAA
                     FKAKFQTHGLYLQPGSNSVHRLYVVVHGGREAVEVFEVDARPQTPTLTWIGCAVAPDP
                     IGLNSVRALPDGGFVATNFLPRGSNMKTLQSGEKNGELWEWHTASGWQKVPGSEASGA
                     NGLEFSTDESRFYVASWGSQSFFRLVWPSASGSQALQATRAKSTPAREEVPLGFRVDN
                     IRWSSDGSILAAGQGGAPGGPQASIVVKINPDTLAVREVVRLPNEQAFGAGTVAVEVG
                     KDLWIGSFRGDRIAIVPAVK"
     gene            complement(2366..2539)
                     /locus_tag="AC16CY_05255"
     CDS             complement(2366..2539)
                     /locus_tag="AC16CY_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353221.1"
                     /translation="MAALIKKDTGLDTELVEGGRGEFSVWVDGERVAQKEAGVFPADQ
                     DAVAAVRRALARG"
BASE COUNT          459 a          898 c          929 g          437 t
ORIGIN      
        1 gcacgcccct tcagcgcagc ctgcgccgag ctgccgatga tcagcatgct tctgcccgag
       61 cgcaggatgc tggccaacag cttcaaggac ggcaggccgc tcggacgcgt caacgacgtg
      121 atggccgacg gcaaaggagg cgcgttcttc acgtctggcg gcgccttcca cgtgagcgcc
      181 gagggcgtcg tgacaacggt ggaggaccag aacatccggt ccaacggcat catgctcagc
      241 ccggacggca agacgctcta cgtcaccaac aacgacgtgg tgctcgcctt cgacgtgcag
      301 cccgacggct ccacgcgcaa tcgccggaac ttcggcgggc tcaacggcga cggcggcggc
      361 gacggcatgg cggtcgatgg caccggccgc ctgtacgtga caggcaacct cggagtgcac
      421 gtgctgtcgc cagagggtaa gtacgtggga ctcatcccca cgccgcgccg gccaatcaca
      481 ctcgccttct cggggcccga caagaagacg ctctacgtgc ctcaaatggg agcggtgggc
      541 cccgacggga aggcgtgggc gacgccggaa ggcattcgca acacggcgat gacgatttac
      601 aggatctcga tgctggccga agggttcaag ggccggccca aatagcgaag gagttatcga
      661 tgcgtacaag actgctggtt tccgtacttg ccgtgatgat cgccaccgcg ctgggcgccg
      721 agctgtcggg acaacagcag ggcgaccggg gcgggggggc gggccgcggc ggtggccgtg
      781 gccgcggatt ggcgccgctg ctcatggaga cggacgcctt cccggacggc ggcgtcgtgc
      841 cgccgaagta cgccggccgc ggcggcgtgc agccgggctt caagttctcg aatgtgccag
      901 aaggcaccgt cagcttcgcg atcatcttcc acgatatcga cgtggcgatc ggcgggaaca
      961 cggacgacgt gctccactgg atcgtgtgga acattcccgc ggcggcaggc ggcattccgg
     1021 agggcaagct gccagacggc tcggttcagg gcgcgaacat cacgaagcag aatgcctact
     1081 tcggccccgg cgcgccggcc ggccctcgct atcaccatta cgtcttcgag ctctacgcgc
     1141 tcaacgccat gctggatctg ccacccacgt cggggcgtgt cgatctgttg aaggcgatgg
     1201 aaggcaaagt ggtcgccaag tcggcgtatg tgggccggtt ccgcggcgag gcgccgccgg
     1261 cgaagtaagt gcctggagta gagagggacc attcatgaaa gcgttgaccc tggcaggtct
     1321 catcgtggcg gcgtgctcgc tcgccgccgt cgcgcagacg ccggcgccgt gcgccgcctc
     1381 cggaagtacg cagttcgtct gcggcgtgca ggcaccggaa gatctcgtcg tcctgccggg
     1441 ggcgcaatgg gtcgtcgcct cggcgtactc cggaagcggc ggcgtctatc tcgtccgcgt
     1501 cagcgacagg acggctatgg tcgcctaccc aggcgcctcg cccagggagc ggctcgacgc
     1561 caagacctac aactcgtgtc ccggtcctcc ggatgccgcc ttcaaggcga agttccagac
     1621 gcacggtctt taccttcaac ccggcagcaa ctcggtccat cggttgtacg tcgtcgtcca
     1681 cggcgggcgt gaagcggtgg aggtcttcga ggtcgacgcg cgtccgcaga cgccgacgct
     1741 gacgtggatc gggtgcgccg tggcgcccga tccgatcggg ctcaactcgg tcagagccct
     1801 gcctgacggc ggcttcgtgg ccaccaattt ccttccgcgc ggcagcaaca tgaagaccct
     1861 gcagtcgggc gagaagaatg gcgagctgtg ggagtggcac accgcctcgg gctggcagaa
     1921 ggttccaggc agcgaggcct ccggcgccaa cgggctcgag ttctcgaccg acgagagtcg
     1981 gttctacgtc gcgtcgtggg gcagccagtc gttcttcagg ctggtgtggc cctccgcgtc
     2041 cggaagccag gcgcttcagg ccacgcgggc gaagtccacg cccgcgcgcg aagaagtccc
     2101 attggggttc cgcgtggaca atatccggtg gtcgagcgac ggctccatcc tggccgcggg
     2161 gcagggaggc gctccaggtg ggccgcaggc gtccatagtc gtcaagatca atccggatac
     2221 tctcgctgtc cgcgaggtcg tccgccttcc gaacgagcag gccttcgggg ccggcaccgt
     2281 ggccgtcgaa gtcggcaagg atctgtggat cggctcgttc cgcggagacc ggatcgcgat
     2341 cgtcccggcc gtgaagtaag cgatctcagc cgcgcgccaa cgcccggcgg acggccgcga
     2401 ccgcgtcctg atcggccggg aacacgcccg cctctttctg ggcgacgcgc tcgccgtcca
     2461 cccacacgct gaactcgccg cgtccgccct cgacgagctc ggtgtcgagg ccggtgtctt
     2521 tcttgatgag cgccgccaca cgggcggcat gcggaagata gtgtcagacc ttgcagtacc
     2581 gaatgcgaat cgccgacatg cacatcatct taccgtgaag agtcccttga ccaacccgcg
     2641 cctcttgcac atgattcccg cgtgactcga tatctggtcg cagcgctcgt cgcgacggcg
     2701 ctcccggcaa cgccgttgga ggc
//
LOCUS       DBNGSH010000230         1826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1376156, whole genome shotgun sequence.
ACCESSION   DBNGSH010000230 DBNGSH010000000
VERSION     DBNGSH010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1376156"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="AC16CY_05260"
     CDS             <1..590
                     /locus_tag="AC16CY_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353222.1"
                     /translation="VYDYNSLNESKYDPEGYCLPPGGPRMMATPYQMEIIQQPEHKRI
                     VMIFEGATHIWREIYMDGRAFPEGDALNPTYLGYSIGRWENPDTLVVETRGFNENSWL
                     DYFGHPHTDQMHVLERWTRPSKQTLRYQATVTDPGAYTRPFTIAWDIPWREGGELPEY
                     ICQENNRFLNRLEDDFGQPIFGPRPQPGQRPSGSR"
     gene            587..997
                     /locus_tag="AC16CY_05265"
     CDS             587..997
                     /locus_tag="AC16CY_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353223.1"
                     /translation="MTRPRFVPALVVVIALAVPVIARAQYGHPLKGQWSGQWGPANNS
                     ARLLLDLNWDGKTVTGMINPGTTDAVVKSVTIDYADPSVWIVKIQAEGKDEAGKPVSI
                     TADGKLENIGAYTRLLHGTWTQGGRKGEFAVTRN"
     gene            1081..>1826
                     /locus_tag="AC16CY_05270"
     CDS             1081..>1826
                     /locus_tag="AC16CY_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3353224.1"
                     /translation="MVIDCHGHYTTAPKQLQTFRDSQIAALKDPRLKPAPGPLKISDD
                     ELRESVQPQLKFQSERGTDVTIFSPRASAMAHHIGDASTSLDWSRHCNDLIHRVCGLF
                     PSNFVGVCQLPQTTSAPLANCIEELERCVTQFGFIGCNLNPDPSGGHWTAPPLTDRSW
                     YPFYEKLVELDVPAMVHVSASCNDNFHATGAHYLNADTTAFMQFLTADLFKDFPKLRM
                     IIPHGGGAVPYHWGRYRGLAQDMKRPPLSEL"
BASE COUNT          348 a          611 c          576 g          291 t
ORIGIN      
        1 cggtctacga ctacaactcg ctgaacgaat cgaagtacga ccccgaaggg tactgcctcc
       61 cgcctggcgg gccgcgcatg atggcgacgc cctaccagat ggagatcatc cagcagcccg
      121 agcacaagcg catcgtcatg atcttcgagg gcgccacgca catctggcgc gagatctaca
      181 tggacgggcg cgcgttcccc gaaggcgacg cgctcaaccc gacgtacctg ggatattcga
      241 tcggccgctg ggaaaacccc gacacgctgg tcgtcgagac cagggggttc aacgagaaca
      301 gctggctcga ttacttcggg catccgcaca ccgatcagat gcacgtcctc gagcgctgga
      361 cgcgaccgag caagcagacg ctgcgctatc aggccacggt caccgatccg ggcgcgtaca
      421 cgaggccttt cacgatcgcg tgggacatcc cgtggcggga aggcggcgag ctccccgagt
      481 acatctgcca ggagaacaac cgcttcctga accggctgga agatgacttc ggccagccga
      541 tcttcggccc gcggccgcag cctggccagc ggccgtcagg atcacggtga cacgcccacg
      601 tttcgttccc gcgctcgtgg tggtgattgc gttggcagtg cccgttatcg cgcgggcgca
      661 gtacgggcac ccgctcaagg gtcaatggag cgggcagtgg gggccggcca acaactccgc
      721 ccggctgctc ctcgatctga actgggacgg caagaccgtc accggcatga tcaaccccgg
      781 cacgaccgac gccgtcgtca agtcggtcac gattgattac gccgacccct ccgtgtggat
      841 cgtgaagatc caggccgagg ggaaggacga ggccggcaag ccggtatcga ttacggccga
      901 cggcaaactc gagaacatcg gcgcctacac gcggctcctt cacggcacct ggacgcaggg
      961 cggccggaag ggggagttcg cggtgacacg caattagtac gtagtgtccg gctagccggt
     1021 cgtacgtagt gtccggcttc agccggacct ggcgtgaact gcggatagaa tcgttagctc
     1081 atggtcatcg actgccatgg gcattacacg actgccccca aacaactaca gacgttccgc
     1141 gacagccaga ttgcggctct caaggacccg cggctgaagc ctgcacccgg cccgctgaag
     1201 atcagcgacg acgagctgcg cgagagcgtg cagccgcagt tgaagttcca gtccgagcgc
     1261 ggcaccgacg tcacgatctt ctcgccgcgc gcgtcggcga tggcgcatca catcggcgac
     1321 gcctcgacga gcctcgactg gtcgcgccac tgcaacgatc tcatccaccg cgtctgcggg
     1381 ctctttccgt cgaacttcgt cggcgtgtgc cagctgccgc agacgacgag cgcgccgctg
     1441 gccaactgca tcgaggagct cgagcgctgc gtgacgcaat tcggcttcat cggctgcaac
     1501 ctgaatcccg atccgtcggg gggacactgg actgcgcccc cgctcaccga tcggagctgg
     1561 taccccttct acgagaagct ggtcgagctg gacgtgccgg ccatggtgca cgtgagcgcg
     1621 tcgtgcaatg acaactttca cgcgaccggc gcccactacc tcaacgcgga caccacggcg
     1681 ttcatgcagt tcctgacggc agatctgttc aaggacttcc cgaagctgcg gatgatcatc
     1741 ccgcatggcg gcggcgcggt gccgtatcac tggggccggt accgcggcct cgcgcaggac
     1801 atgaagcggc cgccgctctc cgagct
//
LOCUS       DBNGSH010000231        24625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_867083, whole genome shotgun sequence.
ACCESSION   DBNGSH010000231 DBNGSH010000000
VERSION     DBNGSH010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 24625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24625
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_867083"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="AC16CY_05275"
     CDS             complement(<1..671)
                     /locus_tag="AC16CY_05275"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024900.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIR3353225.1"
                     /translation="METVSLTIDGQRVTVEKGKTVLQAAIEAGISVPYYCFHPGLTVD
                     GSCRVCIVKIEKMPKLQTSCSTLCTDGMVVTTRDPETTQARAGVLEFLLVNHPLDCPV
                     CDKGGECPLQDFSYSFGPAESRMDFPRRVFDGEGVRADVDFGPTLMLNRNRCILCTRC
                     VRFMKEIDGDAQISIVDRGNGSEIATFREEGVHSLLSGNLMDVCPVGAITTREYRFRS
                     RPWDNP"
     gene            complement(708..3371)
                     /locus_tag="AC16CY_05280"
     CDS             complement(708..3371)
                     /locus_tag="AC16CY_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR3353226.1"
                     /translation="MRLLPLLACAWIWAIPISAQTVEPDPVTPLVRKVEQALNKADAS
                     ALTALFASHVPAAVVDKFIGDLVIKGGVRATVRERERMQLEGAPPGDGYSLVVEFFIE
                     TQGKARILTTGMDMRRPSNGDLASWRFVGVEGLSFVEGLYKLRLDTARPLSARNLQVT
                     AEDLVLLLSEGTVFRVECDDGVTGLILLGRGQMTFSPAVAAERSQLRLFSGDDTLVSP
                     FDFAFVRLNPSDYQNRVSTASLSPAAPDARLARRAEELLAENAPKSFNVDLQDLSTED
                     WYLLPPSRDFLAEIDTRRFDMLSYSVTTENAEDVSVFRRKDRRTLALYPSVAKLAARG
                     RFYTDDVLRDYDVLDYNVETYIDPRRRTLRGRTRLSIRVRSTSLSTLRLRLAESFAVS
                     GVTSVEYGRLLYLRIRSQNAVLVNLPQSVQQDSDLTLIVTYGGEVSTQDLDTDTVTVS
                     PDAQDQGGLGDTREPSLLLSNRSYWYPQNPVPDYATASIRIVVPAGYTCMASGHPAAV
                     IESASFQDGKVAREGQAYAFRANQPLRYLALVISRLVRIGEAKVPIDNQTDPGSGVDS
                     IALAVSSHSSLRGRARDTAKEAEAILRFYASLMGDAPYTSASVALLENDLPGGHSPGY
                     FAVINTPASNITANPWRGDPAAFESFPEFFLAHELAHQWWGQAIGWKNYHEQWISEGF
                     AQYFAALFAQKALGERVFVDMLRQFRKWSIAESDQGPVYLGSRLGHVKSEPRVFRALV
                     YNKAAGVLHMLRSLLGDEVFFRGLRRFYMDRRYQKAGTEDFERVMEAESGRVLDRFFE
                     RWIYGSSIPRVRYQTTIGERDVRVRFEQTGNLVFDLPVTVTLSYGDGRIVNVTVPVTE
                     MEVERSIPIDDVVRRVQINLDGAALAEFDEQ"
     gene            complement(3438..4604)
                     /locus_tag="AC16CY_05285"
     CDS             complement(3438..4604)
                     /locus_tag="AC16CY_05285"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3353227.1"
                     /translation="MKVPPLPRQTGGPFFETYTRGLTTTELERLFTRDTPEAYRLFSR
                     HIDFDELKKKPWHLRVLGTARLLFLAFTLKLSPARRAMYAVALLASLVGLLELYQETH
                     WLLIPHPAFAPGTMWLLTGFLIVNFLVLLEVADRLSLKNDLEIAREIQQSMLPRAAHK
                     APGIEAFGMTRPANTVGGDFYDILTLPDGRVLLALGDVAGKGSPAALLMALLLAMMRT
                     LVDEGFEGADLVARLNLQIGKHAPRTRFVTLFIAVLNPSTGEMTYVNAGQNPPLLRRG
                     NGAYERLRAGGMALGMFEDATYTAGTVDLVPGDVLVMYSDGITEAENTFDQPFDESGV
                     QSVVDGKEWASAKELGWALFAAVETHSQERRLLDDLTVLVARRLPPLPDAMTVV"
     gene            complement(4643..5824)
                     /locus_tag="AC16CY_05290"
     CDS             complement(4643..5824)
                     /locus_tag="AC16CY_05290"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIR3353228.1"
                     /translation="MRTDPLAFLDDELNALRAQGLSRPLRVLDGEQAARLSIDHRSVV
                     NLSSNNYLGFTTHPRLRERALDALKAFGVGSGSVRTIAGTMQIHMELERRLASFKRTE
                     AAVVFQSGFTANAGTVAALLGREDVIVSDELNHASIIDGARLSRAAIKVFPHRDVAAA
                     RAILAALPRSQRTLLVTDGVFSMDGDLGALPGLCDLAEEFGCIMMVDDAHASGVFGAA
                     GRGTIDHFGLHGRVDVQVGTLSKALGALGGYVAGSRSLVEFLYRRGRPFLFSTSHPPS
                     VAATCLAALDVLETEPQWMERLWQNTRFFKSGLHALGFDIGSSESPITPVIVGDGALA
                     MRFSDRLFEAGVFAQGIAFPTVARDKARVRTIVTATHTQADLEFALDAFKTVGIDLGI
                     V"
     gene            complement(5858..7075)
                     /locus_tag="AC16CY_05295"
     CDS             complement(5858..7075)
                     /locus_tag="AC16CY_05295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3353229.1"
                     /translation="MPIRNSQPVPVASRVNRFSYAIRNIVAEATAVEARGTRVRYLNI
                     GDPVAFGFRTPEHMVEAAVRAMRDGRNGYVPSAGIPEARVAVAAEYTSRGVPMSPDRV
                     LITTGTSEAIELALNALAGEGDEVLVPSPTYPLYTAVLAKIGAQPRYYRTDPTRDWLP
                     DLEHLRSLITARTRVLVVIDPNNPTGAVYPAATRRALLEFAERNDLTIMADEVYSDLG
                     FDGPVPLLGTLDLDAPVISLSSLSKAFLAPGWRTGWMAVASTPRLDPALAAMKKLADG
                     RLCSPAPMQYAVKAALGGDRSHQVSFRAALAERARITTDMLNAIPGMTCVAPKAAFYA
                     LPSVSLPPGRTDEEYVLALLRQTGILCVNGSGFGLPAAEGFFRIVFLASPEELSRTYA
                     DIGAFTRDYLSRA"
     gene            7374..8357
                     /locus_tag="AC16CY_05300"
     CDS             7374..8357
                     /locus_tag="AC16CY_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3353230.1"
                     /translation="MARPLIWLRRMRSLAAWVVPIVLPTGLLSAHSAPPNLQQAPQAT
                     FRSGVELVTVTVSVRDQKGRVVRDLTRKDFTVIDSGEGVDIRDFYSGDAPISLAILLD
                     ISGSMAVGGNINRAREAVGVTAKSLRASTDEAALFTFDSGLAEVVSFTRDLERIRRAS
                     LKGTPWGKTSLYDAIGQAATTVGERANRHRALLVITDGVDTGSKRTAAEVSGVASSID
                     VPVYLLTVVTPLDHSGSEFEVSSDGRATQTATLDDLSRWTGGDMRITSTSAHIGAAVR
                     DLLAELRYQYLITFEPGVRRGWHPLEIRTGDESFVVHARGGYMSNPPGGGS"
     gene            8383..9069
                     /locus_tag="AC16CY_05305"
     CDS             8383..9069
                     /locus_tag="AC16CY_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3353231.1"
                     /translation="MRKSLMAGSILALTVAVAPACATKKFVRTEVGNVNSKVDTLAGT
                     LEETQERTRRNEERIGVVDEKAEAAGRSATSARAAADAAANSAREVDGRVTTVNNRVN
                     VVEAAARRLIYEVTLSEDQGNFKFGKTDLPDPAKARLDQVVADLKADPKTIFIEVEGH
                     TDNVGTPDVNERIGLERAEAVKRYLYEQHQVPLHKINVISYGEDKPVAPNNTRDGRAQ
                     NRRVVVKILS"
     gene            complement(9123..9776)
                     /locus_tag="AC16CY_05310"
     CDS             complement(9123..9776)
                     /locus_tag="AC16CY_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353232.1"
                     /translation="MTRLATALCWAVFACMTASALQLKLDRRAIEEALYVGQSRIESE
                     RTRFHEPYRLRVSQPPVDWIDVITPFHRVELAAEANARRGGSQFGQREALDVLRGAPD
                     QIDLMVELTFHPQNTFVGVPAYRVEMVAPGGARVTPRRLDPYPRFGPRLDSSGPQFPA
                     PNAGAAIGGGQPVLGGTLIVAFDGTRLDPKGRYNVVVSEEKGKTELARAALDLARMR"
     gene            9824..10564
                     /locus_tag="AC16CY_05315"
     CDS             9824..10564
                     /locus_tag="AC16CY_05315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3353233.1"
                     /translation="MELDKKVALITGGRRIGAVVATELARRGADLALVYRKSRAEAEE
                     TASAIRAMGRRSVVLQADVREPEACRQAVDAAVDRLGRLDVLVNMASRYKAVPFDELT
                     VQDWDDQLDVDLRAAWLCAKAAAPHMRRLRGGRIINVADWTARSGRPRYHGYLPYYVA
                     KAGVTALTEALALELASDQILVNAIAPGPIVAPEGISDDEFAAVERATPLGRWGGETE
                     IAKMVLACIDSDFVTGETIRVDGGRHLK"
     gene            complement(10557..11402)
                     /locus_tag="AC16CY_05320"
     CDS             complement(10557..11402)
                     /locus_tag="AC16CY_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353234.1"
                     /translation="MLADALRGDAAVIAVELRPPRAELGSAEGMDAWIDTYHEIRRLA
                     RLRVPVFLTDSAVGTQEENNLRHVSANLGRELPRELVNPFLTCKHSLEFCLSYAEQAW
                     QQGFTSLVVLGGDTSVGRPRCVEHAWDLRALIRAREPKLSLGGWANPHASPAKQVDFL
                     LDARFCAEFYLTQVVSHLALEPVTRFLSEVERRGVSRPGMFSVFYYRSANPKTIAALS
                     RFMPVPAEGLAREFSSGATPVDICARTLRALRSLGVRHFCICNLPMHDASGTLSAILE
                     RAGAT"
     gene            11559..12935
                     /locus_tag="AC16CY_05325"
     CDS             11559..12935
                     /locus_tag="AC16CY_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR3353235.1"
                     /translation="MATLTFLGAARTVTGSKYLLEHAGKRVLFDCGLFQGLKELRLRN
                     WADFPVPPGSLDAVVLTHAHLDHVGSLPRLTAHGYKGRVFCTPGTKDLCQLVLPDAGR
                     IQEEDARQANKHGYSKHDPALPLFTESDALRALTLLQPLGYERAIEVAPGISIEFHPA
                     GHLLGSAFVIVRLAKGPTVLFGGDLGRYDRPILPDPSPAMNADVVLVESTYGDRDHRA
                     DDSGEQLAETIRDTAARGGKLVIPSFAIGRVEELLYWVRRLEVERRIPVLPVHVDSPM
                     AAEALRYYSMRVSELDPELRPTHGPVYAFATTRFHVVASTQQSKELTASRASAIVISS
                     SGMATGGRVLHHLEATLPDEKNTALFVGFQAAGTRGRRLVDGEREIRIHGRDVPVRAR
                     IARVDSMSAHADRGEILRWLQTMPSAPGRLCLVHGEPAPMAALKGTIREKLGWAAHSP
                     AHGETLEL"
     gene            12925..14889
                     /locus_tag="AC16CY_05330"
     CDS             12925..14889
                     /locus_tag="AC16CY_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl-peptidase 3 family protein"
                     /protein_id="HIR3353236.1"
                     /translation="MSSEARTHLLECVDDAAVVQLYADGFADLTLPEKTFVWHLYLAA
                     LAGRDIYYDQRYAHSLDMRRVLEGILTHGQDVEPRVLGEVRRYAKLFWINSGPHNNLT
                     ARKFVLSITAEELLEAARSASLAGARLSARADEPVDALIERLAPLFLDDSVDPFVTSK
                     SPGEGRDMLQASSNNLYSGVSVRDLDSFEERYPLNSRLKGENGRLVEEVYRVGGRYDR
                     EIRRIIGHLRDAIPFATPPAAESLRALIRFYETGEEADRIAYDIAWVGDSESPVDTIN
                     GFVEVYLDPRGAKGAWEGIVCYVHAAKTRALQTLAVHAQWFEDHMPWDARFRKTDVKG
                     ITVRAIEVVVETGDSGPVSPVGINLPNDQAIRERHGSKSMSLTNVSDAYERSTPDDMR
                     TEFAWSPDEADRSRRWGGFVQELTTAMHEVIGHGSGRMAPGVADSPSRLLAEQYSAIE
                     EARADLVALYFLPDAKLVTLGLVPAGEQDDIVKAEYEHYVRNALVQLRRVREGTKLEE
                     DHMRSRQMIVNWLRAHTTSVDVRRRDGNTYFVMTDRAAFREGVGRLLAEVQRIKSEGD
                     LAAARGLFETYGVHFDPALRDEVVTRVDRLRLPSYTGFVMPRLHARWNEAGAIVDVEM
                     SYPCDLEAQMLEYSAFSRHDDVLQALSSSR"
     gene            14858..16036
                     /locus_tag="AC16CY_05335"
     CDS             14858..16036
                     /locus_tag="AC16CY_05335"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3353237.1"
                     /translation="MFSKRFPRHAEINELTRTLQRLRAEGASITDLTDSNPTRVGVTY
                     PDDLLDDLSSEAARRYEPHSLGLRSAREAVAADYGRRGARVDPDHVVLSASTSEAYSW
                     IFKLLCDPGQAVLVPEPSYPLFEHLTGAEGVKAVAYQLRYHGRWEIDIDSLAAAPSDT
                     RAVLLVSPNNPTGSFVSRLEANRMAALCRERGWAIVADEVFADYALDEAAAVTECAMN
                     LGVLSLTLGGASKTLGLPQVKLAWMVVDGPPADRDRALGALELIADMYLSVSTPVQVA
                     APSLLARGEAIRRQIRARVAANLARARQLIPRYPACTPLPVEGGWSLVVRVPSTRSEE
                     ALVLDLLERERILVHPGYFFDFPHEAFIVVSLLPPEEVFADALDRALCVATSVGGGRP
                     "
     gene            16033..17625
                     /gene="malQ"
                     /locus_tag="AC16CY_05340"
     CDS             16033..17625
                     /gene="malQ"
                     /locus_tag="AC16CY_05340"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR3353238.1"
                     /translation="MNPPGPRRAGILVPLFSIPSGRSWGIGEIGDLERLVSWLASAGQ
                     RVLQLLPITEVSPGDASPYGSLSAMAIDPQYITLDALEDFAATGGEGALAPEERVALD
                     RARAAPAIDHRLVRDLKQAALRRAFAHFVSREWAGGTAKAAALRAFTAEQAWWLEDYA
                     LFRALHARHDERPWMEWPEPIRSRQPHVIEIEHLALGKEILFRQYLQWVAGEQWAHAR
                     AHARACGVAIFGDLPFVVSADSADVWARQDEFLLEESVGAPPDAFSETGQDWSLPPYR
                     WEVVTARDFSWLRERVRRFANLFDGCRVDHVVGFYRTFVRPPGGGAGRFSPADEASQL
                     SLGERALAALTSAPMQIVAEDLGTVPDFVRESLARLSIPGCKVFRWERRWNEPGQPFK
                     DPIEYPAAAMATSGTHDTESMAVWWEQAPVEERRAVLAIPSVRARMSEDDRARAVDAS
                     ALSPATRAILLEILFASRSTLLILPIQDVFGWSDRINRPGTIGDHNWTWRLPWPSDRM
                     SAQPEAAAAADQLREWSRRYER"
     gene            complement(17613..19316)
                     /locus_tag="AC16CY_05345"
     CDS             complement(17613..19316)
                     /locus_tag="AC16CY_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR3353239.1"
                     /translation="MPEPPTAVDPARSQDFIRDIIAEDLRAGRHASRVHTRFPPEPNG
                     YLHIGHAKSICLNFGVAAEFGGLCNLRFDDTNPTKEDVEYVDSIQADVRWLGFDWSSR
                     MFYASDYFEQMFLYAEQLIQDEKAYVDSLSADQIREYRGSLTAPGKDSPYRARRIEEN
                     LDLFRRMRAGEFPDGTHVLRAKIDMASPNINMRDPTLYRIRHATHHRTGDAWCIYPTY
                     DYAHPLSDALEGITHSLCTLEFEDHRPLYDWVVENVRHDAAFRSRPQQIEFARLNLNF
                     TVMSKRKLLALVEQGLVRGWSDPRLPTIAGIRRRGYTPEAVRDFCTRIGVAKKENVID
                     VALLEHTVREDLNRRAQRALAVLQPLRVEIDNVPEGHVEYVDAVNNPENPSAGHRTIP
                     FSRVLYIEREDFMENPPKKFFRLSPGNEVRLRYAYILKCEQVVKDADGVPIELRCTID
                     RGSLNGETASRRVKGTIHWVSAAHASDAEVRLYDRLFRSDDPGEGGRDPLSDLNPDSL
                     RVLTGCKVEEMLAGASAGATFQFERQGYFTVDPDSAPGAPVFNRTVTLKDTWARISQR
                     S"
     gene            complement(19511..20755)
                     /locus_tag="AC16CY_05350"
     CDS             complement(19511..20755)
                     /locus_tag="AC16CY_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR3353240.1"
                     /translation="MSEWLIASAVTALVVFAVMRSVALRQVADARAAAAAELAAARQD
                     LKWVREDAGRQKEALESAKSLVDRAEQRLRDTFQSLAADALKDNRASFFDLAKTSFEG
                     YRQPIADTLRRVDQRLDTAERERVEAYSRLSEQVIALGSTASTLSRALRTPAVRGRWG
                     EMQLRRVVEISGMLHQCDFDEQPGLASDNGRFRPDLVVHLPGNKQIVVDAKTPLEAFL
                     EAQECTDDEQRALRLQGHARQVREHMDRLGSKAYWDELPNSPEMVVMFLPGETLFGAA
                     LQQDLTLIEHGLEQRVLLASPITLIALLTTVAHSWRHEALTENYREVARLGKEFYERL
                     ATFADRFDDVRKRLDGAVQAYNEAAGSFESRVLVSARRLRDLDVTTAPELPPADPIER
                     VPRVLKQAGLMGLPEGALTDDD"
     gene            20815..21792
                     /locus_tag="AC16CY_05355"
     CDS             20815..21792
                     /locus_tag="AC16CY_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353241.1"
                     /translation="MPAELHCGFTNSGPNPRSESGAVVAAASVVVSRVAHEAPSIRQD
                     GFGVRQIEDVPRAGRVERLAVSPALATPTSEQAIRARAAHLNRLGPGPIGRVAKIERK
                     ASGLIITSLLPDGVPLSDILAALEFGTLTLSDDEVLELAASVVRGAARMHEMLGSLAH
                     GALTPAHVVLMRDGTSVFTGAVFGDAIQSLQRNRESIWREFGLALPAAAAAPRFDKRS
                     DVTQLGAIVLAIALRRSLRRDEFPRGIGDLIMLTTAASTFDGNSRLHMWLQDGLQLHG
                     RVVFDSAVDASRAFDRLLPDDCGDGAEALALRTAILQMSGASSSGASAA"
     gene            21902..23104
                     /locus_tag="AC16CY_05360"
     CDS             21902..23104
                     /locus_tag="AC16CY_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3353242.1"
                     /translation="MGRLTIALALMPWLAACGGQAPPAPVLPDVYLAPDTTIARGVVA
                     PGSTLETMLLDQGLETGVVHGVIDAVRMVFDPRRLRSLQPFLLELSLDGALRLFEYEI
                     DAASFLRVGPGRDAANALHAEVVPIPSTLVHESVRGAIDDATPSLFQSVEAVGEQPDL
                     AIAMAQIFSGEIDFTSELQPGDRYAIAFERFERDGRPSSYGAITAAEFRNEGRVIRAI
                     RFTPAGGEPGYYDEEGRSLRRFFLKSPLKFEPRVTSGFSSRRMHPVLHQARAHRGVDY
                     GAPRGAEVVSVASGRVLSASFDNANGRMVRIRHTSGYVSMYLHLSSFASGVRAGAAVA
                     QGQTIGRVGSTGLATGPHLHYGLQKNGVFVNPLREHRNMPPGEPVPSADRAAFNVERD
                     RAQSLFSQ"
     gene            23118..24338
                     /locus_tag="AC16CY_05365"
     CDS             23118..24338
                     /locus_tag="AC16CY_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR3353243.1"
                     /translation="MAALVPFRALRPAATSAARVAAVPYDVVNTSEARALASDNPLSF
                     LHVSRAEIDLPDGASPYADAVYARAAANFDRLRQQAPLVMEDAPRLYVYRLRMGRHSQ
                     TGLAACFSVDEYERRVILRHEHTRPDKEDDRTRHLLTLGAQTGPVFLTYRARPAVDGL
                     VERVTTTTPVYDFTAIDGVQHTIWLVGAAEERQIILAFDAIPALYIADGHHRAASAAR
                     ARRTLAGAAHATGEWDWFLAVAFPDSEVQILPYNRVIADLAGMTPAAFLDALRARALV
                     QSGPAEPQRRGEVAMFLQGSWHTIVLPDAPAAVSPADRLDVTRLQATVLEPLLRIGDV
                     RTDKRIDFVGGVRGTAELEARVRSGVAALAFSMFPVDAADLMAIADAGGIMPPKSTWF
                     EPKLRDGLLSHVIS"
     gene            24343..>24625
                     /locus_tag="AC16CY_05370"
     CDS             24343..>24625
                     /locus_tag="AC16CY_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3353244.1"
                     /translation="MTTTTRVFNFSAGPAVLPLPVLEQAQRELVALPGVGMSVMEISH
                     RSRTFEDMLQSTIAGIRDLAGVPSNYKILFLQGGASLQFTMVPMNLLRAG"
BASE COUNT         4132 a         8097 c         8265 g         4131 t
ORIGIN      
        1 gggttgtccc agggccggct cctgaagcgg tactccctcg tcgtaatcgc tccgacggga
       61 cacacgtcca tcagattgcc cgagagcagc gagtgcacgc cctcctcgcg gaacgtcgcg
      121 atctcgctgc cgttgccgcg gtcaacgata ctgatctgcg cgtcgccgtc gatctccttc
      181 atgaagcgga cgcaccgcgt gcacaggatg cagcggttgc ggttcagcat cagcgtggga
      241 ccgaaatcga cgtccgccct tacgccttcg ccgtcgaaga cccggcgcgg aaagtccatc
      301 cggctttcgg cggggccaaa gctgtaggag aaatcctgga gcgggcactc accgcccttg
      361 tcacacaccg ggcagtcgag cgggtggttg acgagcagga actcgagcac gcccgcgcgg
      421 gcctgtgtgg tctccggatc ccgcgtggtg acgaccatgc cgtcggtgca gagcgtcgaa
      481 cacgacgtct gcagcttcgg catcttctcg atcttcacga tgcacacgcg gcacgagccg
      541 tcgacggtca accccggatg aaagcagtag taggggaccg agatgccggc ttcgatggca
      601 gcctgcagga cggtctttcc cttctcgacc gtcacgcgct gcccatcgat ggtcagcgaa
      661 acagtttcca tatccgggcg atcatatccc gaggctcggg cgcccgccta ctgttcgtcg
      721 aattccgcga gggccgcccc gtccaagttg atctggacgc ggcgcacgac gtcgtcgatg
      781 gggatggacc gctcgacttc catctccgtg acgggcaccg tgacgttgac gattcggcca
      841 tcgccgtagg acagcgtcac ggtgacgggc agatcgaaga cgaggtttcc cgtttgctcg
      901 aacctgaccc gtacgtcccg ctcaccgatg gtggtctggt atctcacacg ggggatgctg
      961 ctcccgtaaa tccagcgctc gaagaagcga tcgagcacgc ggcccgattc ggcttccatg
     1021 acacgctcga agtcctctgt gcccgccttc tgataccggc ggtccatgta gaacctgcgc
     1081 aggccgcgga agaacacctc gtcgccgagc aggcttctca gcatgtggag gacgccggcg
     1141 gccttgttgt agacgagtgc gcggaagacc cgcggctcgc tcttgacgtg gcccagccgg
     1201 gagccgaggt acaccggccc ctggtctgat tctgcgatcg accatttgcg gaactgccgc
     1261 agcatgtcca cgaacacgcg ctcgcccaac gccttttgcg cgaacagcgc ggcgaaatac
     1321 tgggcgaagc cctcgctgat ccactgttcg tggtagttct tccagccgat cgcctggccc
     1381 caccactggt gtgcgagctc gtgcgcgagg aagaactccg gaaaactctc gaacgccgcc
     1441 gggtcgccgc gccaggggtt tgcggtgatg ttggaggcgg gcgtgttgat gacggcaaag
     1501 tagcccgggc tgtgtccgcc gggcagatcg ttttcgagca gcgccacgct cgctgacgtg
     1561 tacggcgcgt cgcccatgag cgatgcgtag aaccgcagga tggcctcggc ttccttcgcc
     1621 gtgtctcggg cccgaccgcg caggctgctg tgcgacgaca ctgcaagagc gatcgagtcg
     1681 acgcccgagc cgggatcggt ctggttgtca atgggcactt tggcttcacc gatgcgcacg
     1741 agccgcgaga tcaccagcgc tagataccgc agcggctggt tggcgcggaa ggcgtacgcc
     1801 tgaccctcgc gggcgacctt cccgtcctgg aatgaagccg actcgatcac cgcggccgga
     1861 tggccgctcg ccatgcacgt gtagccggcc ggaacgacga tccggatcga ggccgtggcg
     1921 tagtccggaa cgggattctg cggataccag tacgaacggt tgctgaggag caacgacggc
     1981 tcgcgcgtgt cgccgagccc cccttgatcc tgcgcgtcgg gcgagacggt cacggtgtcg
     2041 gtgtcgagat cctgcgtcga cacctcgccc ccataggtca cgatgagggt gaggtccgag
     2101 tcctgctgga cgctttgcgg cagattcacc aggaccgcgt tctggctgcg gatccggagg
     2161 tagagcaggc ggccgtactc gacgctcgtg acgccgctga ccgcgaacga ttccgccagg
     2221 cgaagcctga gcgtggacag cgaggtcgag cgcacgcgga tcgacagccg cgtacggccg
     2281 cgcagcgttc gccgtcgagg gtcgatatag gtctcgacgt tgtagtcgag cacgtcatag
     2341 tccctcagca cgtcgtcggt gtagaagcgc ccgcgcgccg cgagcttggc gaccgacgga
     2401 tagagcgcca gcgtccggcg atcttttctt cggaacacgg acacgtcctc ggcgttctcg
     2461 gtcgtgacgg agtaggacag catgtcgaag cggcgcgtat cgatctcggc gagaaaatcg
     2521 cgggacggcg gaaggaggta ccagtcctcg gtgctgaggt cctgcagatc cacattgaag
     2581 gacttcggcg cgttctcggc cagcagctcc tcggcgcgtc ttgccagccg cgcatccggg
     2641 gcggccggcg agaggctggc cgtcgacacg cggttctggt agtcgctcgg gttgaggcgc
     2701 acgaaggcga agtcgaaggg cgagaccagc gtgtcgtcgc ccgagaagag tcgaagctgg
     2761 ctgcgttccg cggcgacagc cggcgaaaac gtcatctgcc ctcggccgag caggatcaga
     2821 ccggtgacgc cgtcgtcgca ttcgacccgg aagactgtgc cttccgaaag caggagcacg
     2881 aggtcttccg ccgtgacttg cagattacgc gccgacagcg gacgcgcggt gtcgagacgc
     2941 agcttgtaca gaccctcaac aaacgacagg ccttcgacgc cgacgaaccg ccacgaggcc
     3001 aggtcgccat tggaggggcg gcgcatgtcc atgccggtcg tcaggattcg cgccttgcct
     3061 tgcgtctcga tgaagaactc gacgaccagg ctgtagccgt cgcccggcgg cgccccttcg
     3121 agctgcatcc gctcgcgctc gcgaacggtc gccctgacgc cgcccttgat gacgagatcg
     3181 ccgatgaact tgtcaacgac cgcagcaggc acgtgcgagg caaagagcgc cgtcagcgcg
     3241 gaggcatcag ccttgttcag cgcttgttcg accttgcgta cgagcggcgt aaccggatcg
     3301 ggttcgacgg tctgagctga tatggggatc gcccagatcc aggcgcacgc cagcagcgga
     3361 agaagtcgca tatcgctggg gccggcacca ttagacgcca attgccttcc ggccgccact
     3421 ggcggtttga ggctgcgtta cacgacggtc atcgcgtccg ggagcggcgg aagtcggcgc
     3481 gcaacgagaa cggtcaagtc gtccagcagc cgccgctcct gactgtgcgt ttcgacggcc
     3541 gcaaacagtg cccaacccag ttccttggcc gatgcccatt ccttgccgtc gacgacggac
     3601 tggacgccgg actcgtcgaa cggctggtcg aaggtgttct cggcttccgt gatgccgtcg
     3661 ctgtacatca cgagaacatc ccccgggacg aggtcgacag taccggcggt atacgtggcg
     3721 tcctcgaaca tgccgagggc catgccgccc gcgcgcagac gttcgtaggc gccgttcccg
     3781 cggcgcagca aaggagggtt ctgcccggcg ttgacatagg tcatctcgcc ggtcgatggg
     3841 ttcaggacgg caatgaacag cgtcacgaat cgggtgcgcg gcgcgtgctt cccaatctgc
     3901 aggttcaacc gggccaccag gtctgcgccc tcgaagcctt cgtccacgag cgttcgcatc
     3961 atcgccagga gcagcgccat cagcagtgcc gccggacttc ccttccctgc gacatcgccg
     4021 agcgccagca gcacgcgccc gtcggggagt gtgaggatgt cgtagaagtc gccgccaacg
     4081 gtgttcgccg gccgcgtcat cccgaacgcc tcgatcccgg gcgccttgtg ggcggctcgc
     4141 ggcagcatcg actgctgaat ctcgcgcgcg atctcgaggt cgttcttcag ggacagccgg
     4201 tcggccactt ccagcagcac gaggaagttc acgatcagga acccggtgag cagccacatg
     4261 gtaccgggcg cgaacgcagg atgcgggatc agaagccagt gcgtttcctg atagagctcg
     4321 agaaggccga cgagactcgc caggagcgcc accgcgtaca tcgcgcgcct cgcgggagac
     4381 aacttgagcg tgaacgccag aaagagcagc cgcgcggttc caagcacgcg caggtgccac
     4441 ggcttctttt tgagctcgtc gaaatcgatg tgcctcgaga acagccgata cgcttcgggt
     4501 gtatcgcggg tgaacagacg ctcgagctcg gtggtcgtga gccccctcgt ataggtctcg
     4561 aagaacgggc caccggtctg acgcggcaaa ggagggactt tcatcgacgc tcggatctta
     4621 gatcggccgc gggggcggcg cttcagacaa ttccgagatc gatcccgaca gtcttgaagg
     4681 catccagcgc aaactcgagg tctgcctgcg tgtgggttgc ggtcacaatc gttcgaaccc
     4741 tggccttgtc gcgcgcgacg gtagggaagg cgatgccctg tgcgaacacc ccagcctcga
     4801 acaaacggtc ggagaacctc atggccagcg cgccgtcccc cacgatgacc ggcgtgatcg
     4861 ggctttcgct cgacccgatg tcgaagccaa gcgcgtgcag ccccgacttg aagaaacggg
     4921 tgttctgcca gaggcgttcc atccactgcg gttcggtctc gagcacgtcg agcgccgcaa
     4981 ggcacgttgc ggcgaccgat ggcgggtggg acgtggaaaa cagaaagggc ctgccccggc
     5041 ggtacaggaa ctcgacgagc gaccgcgagc ctgcgacgta gcccccgagc gcgccaagcg
     5101 ctttcgagag ggttcccact tgcacgtcta ctcgcccgtg aagcccgaag tgatcgatgg
     5161 tgccacgccc cgcggctccg aacactccgc tggcgtgggc gtcgtcgacc atcatgatgc
     5221 agccgaactc ctcggcgaga tcgcacaatc ccggcagggc gccgagatcg ccgtccatgc
     5281 tgaacacgcc gtcggtcacg agcagcgtgc gctgcgagcg aggcaatgcc gcgagaatcg
     5341 cgcgcgcggc ggcgacgtcg cgatgcggaa acaccttgat ggcagcccgg ctcaggcgag
     5401 ccccgtcgat gatgctcgcg tgattcagtt cgtccgagac gatgacgtcc tcgcggccga
     5461 gcaacgccgc caccgttccc gcgtttgccg tgaagccgct ctggaagacg accgctgcct
     5521 cggtgcgctt gaacgatgcg aggcggcgct cgagctccat gtggatctgc atcgtgccgg
     5581 caatcgtgcg gaccgatccg ctgcccaccc cgaacgcctt gagcgcgtcg agcgcgcgct
     5641 cgcggagccg cggatgggtc gtgaagccga gatagttgtt cgacgacagg ttgacgacgg
     5701 accggtgatc gattgaaaga cgagccgctt gttcaccgtc gagtacacgc agcggccggg
     5761 acagaccttg cgcgcgcagc gcgttcagtt cgtcgtcgag gaatgcgagc ggatccgtgc
     5821 gcatgcggca ccggcccttt tccgtcgcgg tccgccgcta cgcgcgcgac aggtagtcgc
     5881 gcgtgaaggc gccgatgtcc gcgtaagtcc tggacaactc ctcggggctc gccaggaaga
     5941 caatccggaa gaacccctcg gccgccggca ggccgaaccc ggatccgttg acgcacagga
     6001 tgccggtttg ccggagcagc gccagcacgt actcctcgtc ggttcggccc ggcggcaacg
     6061 ataccgacgg cagcgcgtag aacgcagcct tcggcgcaac gcaggtcatg ccggggatgg
     6121 cgttcagcat gtcagtcgtg atccgggccc gctccgcgag cgccgctcga aatgacacct
     6181 gatgcgatcg gtcgccgcca agcgccgcct tcaccgcgta ctgcatcgga gccgggctgc
     6241 agagccgtcc gtccgcgagt ttcttcatag cggccaacgc ggggtcaagt ctgggcgtgg
     6301 acgccacggc catccacccc gtgcgccacc ccggcgccag gaatgccttc gagaggctcg
     6361 acaaggagat cacgggagcg tcgaggtcca acgtcccgag cagcggtacc ggcccgtcga
     6421 accccaggtc gctgtacact tcgtcggcca tgatcgtcag atcgttccgc tcggcgaact
     6481 cgagaagcgc ccggcgggtc gcggccgggt acacggcgcc cgtcggattg ttcgggtcga
     6541 tgacgacgag cactcgcgta cgcgccgtga tgagactgcg aagatgctcg agatcgggca
     6601 gccagtcgcg cgtcggatcg gtgcggtagt accgcggctg agcgccaatc ttcgccagaa
     6661 ccgccgtata aagcgggtag gtcggcgacg ggacgagtac ttcgtctcct tcaccagcca
     6721 gcgcgttcag tgccagctcg attgcttccg aggttccggt cgtgatgagc accctgtcgg
     6781 gggacatggg cacaccgcgg gaggtgtact cggccgccac ggccacgcgc gcctccggga
     6841 tgcccgccga agggacataa ccgtttcgtc cgtcgcgcat cgcgcggacc gccgcttcga
     6901 ccatgtgctc cggtgtgcga aacccgaacg ccacagggtc cccgatgttc agatagcgaa
     6961 cgcgcgtccc gcgcgcttcg acggccgtcg cctcggcgac gatgttgcgg atcgcgtagg
     7021 agaaccggtt aacgcggctt gcgaccggaa cgggctgaga gtttcgtatg ggcatcactg
     7081 gataaggcta accacaattc ggccaacggg ttggctccga agtcgaggcg aggagcggat
     7141 cccttggagt cgaggtcagg caaatattac gtaagcctcg accccgcagg taactgcgac
     7201 cactctgaaa taatggcaga actcgtgaag ctttgcccgc ccctctcgcg aaatacatcg
     7261 gtgcatcgtc cgaagtcctt ccgtagcgta agagttacat ctgaggagct tgcgcatacc
     7321 tgcccaggat agggaattgg ccgtaagttg ttgagccata acaagcgtcg tctatggcaa
     7381 ggccattgat atggctcagg cgcatgcgga gcctggcggc ttgggtcgtt cccatcgtgc
     7441 tacccacggg gctcttgtcc gctcactccg ccccgcctaa cctgcaacag gctcctcaag
     7501 ccacgtttcg gtcgggcgtc gagctcgtaa cagtcaccgt ttcggtcagg gaccaaaagg
     7561 gccgcgtcgt ccgggacctg acccggaagg atttcacggt catcgatagc ggagagggag
     7621 tcgacatccg agacttctat tcgggtgacg cgcccatcag cctggccatc ctcctcgaca
     7681 tcagcggcag tatggcggtc ggcggcaaca tcaaccgcgc ccgcgaggct gtaggcgtga
     7741 cggcaaagag ccttcgcgcc tcgaccgacg aggcagccct gttcacgttc gactcgggtt
     7801 tggcggaagt cgtcagcttc acgcgagacc tcgagcgcat ccgacgcgca agcctgaaag
     7861 ggacgccgtg gggcaagacg tccttgtacg atgcgatcgg tcaggcggcg accaccgtgg
     7921 gcgagcgcgc caacaggcat cgcgcgctgc tcgtcatcac cgatggcgtc gataccggaa
     7981 gcaaacggac cgcggcagag gtctcgggtg tcgccagctc aatcgacgtc cccgtgtacc
     8041 tgttgactgt cgtgactccg ctggaccact cgggcagcga gttcgaggtt tcaagcgacg
     8101 gccgggcgac gcagaccgcg acgctcgacg atctgtcgcg ctggaccggc ggtgacatgc
     8161 gcatcaccag cacgtctgcg cacatcgggg cggccgtccg ggatctcctc gcggagctgc
     8221 gctatcagta tttgatcaca ttcgagccgg gtgttcggcg cgggtggcac ccgctcgaga
     8281 ttcgtacggg agacgagagc tttgtggttc acgcgcgcgg cggttacatg tccaacccgc
     8341 cgggcggcgg aagctagcta gtacgtacag agggagaaca tcatgcggaa gagtctgatg
     8401 gcaggatcga tcctcgcgct gaccgtggcc gttgccccgg cctgcgcgac caagaagttc
     8461 gtgcgcacgg aagtcggcaa tgtgaactcc aaggtggaca cgttggccgg cacgctcgaa
     8521 gagacgcagg aacggacgcg ccgcaacgaa gagcgcatcg gcgtcgtcga cgagaaggcc
     8581 gaggcggcgg gtcgctccgc cacatcggct cgggcagcgg cagacgcggc tgcaaactca
     8641 gccagggaag tggacggccg agtcaccacg gtgaacaatc gcgtgaacgt ggtggaagcg
     8701 gcggcacgcc ggctgatata cgaggtgact ctcagcgagg accagggcaa cttcaagttc
     8761 ggcaagacgg atctgccgga tcctgccaag gcgcgcctcg accaggtggt cgccgacctc
     8821 aaggcggatc cgaagaccat cttcatcgag gttgaggggc atactgacaa cgtggggacg
     8881 cccgacgtga acgagcggat cggtctcgag cgagccgagg cggtcaagag gtacctgtat
     8941 gaacagcacc aggtgccgct ccacaagatc aacgtgatct cctacggcga ggacaagccg
     9001 gtcgcgccga acaacacccg tgacggtcgc gcgcagaacc gccgcgtggt ggtcaagatc
     9061 ctgtcgtagt cgcctgccaa gctggtggag cggatccgtt cccggcgagg gacggattcg
     9121 ctctaccgca tcctcgccag atcgagcgcc gcgcgagcga gctcggtctt tcccttttcc
     9181 tcgctgacga cgacgttgta gcgccctttc gggtcgagcc tggtgccgtc gaacgccacg
     9241 atgagcgtgc cgccgagcac tggttgccct ccgccgatag ctgctccggc attcggcgcg
     9301 gggaactgtg ggcctgacga atcgaggcgc ggcccgaagc gcgggtatgg atccaggcgc
     9361 ctgggagtga cgcgcgcgcc tcccggcgcg accatctcca cccggtacgc cggcacgccg
     9421 acgaacgtgt tctggggatg gaaggtcagc tcgaccatca ggtcgatctg gtccggcgct
     9481 ccgcggagaa cgtcgagtgc ctcccgctgc ccgaattgcg atccccccct gcgtgcgttc
     9541 gcttcagccg cgagttccac gcggtggaac ggcgtgatca cgtcgatcca atcgaccggt
     9601 ggctgcgaca cccgtagtcg atagggctcg tgaaacctgg tgcgttctga ttcgatccgg
     9661 gattggccga cgtagagagc ttcttcaatg gcccgccgat cgagcttcag ctgaagcgcg
     9721 ctcgcagtca tgcacgcgaa caccgcccag cagagcgccg ttgcaagccg agtcatctcg
     9781 acgcatcgta gcagagaggc cgcggggtcg ttagtattgg gccatggaac tcgacaagaa
     9841 ggtggccctg atcaccggcg gccggcggat tggggcggtc gtggccaccg agcttgcgcg
     9901 gcggggcgcc gaccttgcgc tcgtgtaccg caagtcccgt gcagaagccg aggagaccgc
     9961 cagtgccatc cgtgcgatgg ggcggcgatc ggtggtgctg caagctgacg tccgcgagcc
    10021 cgaagcctgt cgtcaggccg tcgatgccgc ggtcgatcgc ctcggacgcc tcgacgtgct
    10081 cgtcaacatg gcgtcgcgat acaaggccgt acccttcgac gagctgaccg tccaggactg
    10141 ggacgatcag ctcgatgtgg atcttcgcgc cgcctggctg tgcgcgaaag cggcagcgcc
    10201 tcacatgaga cgtttgcgcg gcggccgaat catcaacgtc gccgactgga ccgcgagaag
    10261 cggccggccg aggtaccacg gctaccttcc ctactacgtc gccaaggccg gcgtcacggc
    10321 gctgaccgaa gcgctcgccc tggagcttgc ctcggatcag atcctggtga acgcgattgc
    10381 gcccgggccc atcgtcgcgc cggaaggcat ctccgacgac gagttcgcgg cggtggaacg
    10441 ggccacgccc ctcggacgat ggggcggcga gacggagatc gccaagatgg tgctcgcgtg
    10501 catcgacagc gacttcgtta ccggcgaaac catccgcgtg gacggcggcc ggcaccttaa
    10561 gtagcgccgg ctcgctcgag gatggccgac agcgtgccgg aggcgtcgtg catgggcagg
    10621 ttgcagatgc agaagtgccg tacccccagc gacctcaatg cacgcagggt ccgtgcgcag
    10681 atgtcgacgg gcgtggcgcc ggacgagaac tcgcgcgcga ggccctcggc aggaaccggc
    10741 atgaagcggc tcagcgccgc gatcgtcttc gggtttgcgc ttcggtaata gaacacgctg
    10801 aacatcccag gccgcgagac gccacggcgc tcgacctcgc tcaggaaccg ggtgaccggc
    10861 tcgagcgcga gatggctgac gacctgcgtg agatagaact cggcgcagaa acgggcgtcg
    10921 aggaggaagt cgacctgttt cgccggcgag gcgtgcggat tggcccaccc gccgagcgac
    10981 aacttcggct cgcgcgcgcg gatcagcgcc cgcagatccc acgcgtgctc gacgcatcgc
    11041 ggtcggccca ccgaggtgtc gccgccgagc acgaccagcg aggtgaaccc ctgctgccag
    11101 gcctgctccg cgtacgagag gcagaactcc agggaatgct tgcacgtcag gaacgggttc
    11161 acgagctccc tcggcagctc ccggccgagg ttggcgctga cgtgacggag gttgttctct
    11221 tcctgcgtcc cgaccgcgct gtcggtgaga aacaccggaa cgcgaagccg cgccagacgg
    11281 cggatctcgt ggtaggtgtc gatccacgcg tccatgcctt ccgccgagcc gagctccgcc
    11341 cggggcggcc gcagctccac ggcgatcacg gcggcatcgc cccggagggc gtcggccagc
    11401 atcaacctac ggtgacggtg actcgctcag gctggacgca cgcatcacga aatcgaacat
    11461 agtggggccg agcgtcgata cgctccacca gtcatgttgc acagagtaca ccgggcagtg
    11521 cgtctgtgcg ccaatccttg atatcgtcgt cttacgacgt ggccacgctc acattccttg
    11581 gtgcggcacg caccgttacc ggctccaagt acctgctcga gcacgcgggg aaacgggtgc
    11641 tgttcgattg cggactgttc cagggcctca aggaactgcg gcttcgtaac tgggctgact
    11701 ttcctgttcc ccccggctcg ctcgatgcgg tcgtactcac gcacgcgcat ctggaccacg
    11761 tgggctctct gccgaggctg accgcccacg gctacaaggg gcgcgtgttc tgcactcccg
    11821 gcaccaagga tctctgtcag ctcgtgctgc cggacgccgg tcgaatacag gaagaggacg
    11881 cgcgtcaggc gaacaagcac ggttactcga agcacgatcc ggcgctgccc ctgttcacgg
    11941 aaagcgacgc cttgagggcg ctgaccctgc ttcagccgct cggctacgaa cgagccatcg
    12001 aggtcgcgcc gggaatctcg atcgagtttc atccggcggg ccacctgctt ggctcggctt
    12061 tcgtgatcgt ccggctcgcc aagggtccga cagtgctctt cggcggagac ctcggccgct
    12121 acgaccggcc gatcctgccc gatccctcac cggccatgaa cgccgacgtg gtcctcgtgg
    12181 agtccaccta cggcgaccgg gatcaccggg cggacgattc gggcgagcag ctggccgaaa
    12241 ccatacgcga taccgcggcg cggggcggga agctcgtgat cccgtcgttt gccatcggcc
    12301 gcgtggagga gttgctgtac tgggtacggc gactcgaggt cgagcggcgc attccggtgc
    12361 tgccggtcca tgtggacagc ccgatggccg ccgaggcgct gaggtactac tcgatgcggg
    12421 tgtctgagct cgatcccgag ctgcggccga cccacgggcc cgtgtacgca tttgccacca
    12481 cgcggttcca cgtcgtcgcc tccacgcagc aatcaaaaga gctgacggcc agccgcgcga
    12541 gcgccatcgt catctcgtcg agcgggatgg cgacgggcgg ccgcgtcctg caccacctgg
    12601 aggcgacgct gccagacgaa aagaacacgg cattgttcgt cgggttccag gccgcgggca
    12661 ccagagggcg aaggctggtg gacggagagc gcgagatccg gattcacggc cgcgacgttc
    12721 ccgtgcgtgc gcgcattgcc cgagtcgatt cgatgtccgc ccatgccgac cgcggcgaga
    12781 ttctccggtg gctccagacg atgccttccg cgccgggccg gctctgcctg gtccacggcg
    12841 aacccgcgcc gatggccgcg ctcaagggga ccattcgcga gaagctcggg tgggccgcgc
    12901 actcaccagc gcatggagaa acccttgagc tctgaggcgc gcacccacct cctcgaatgc
    12961 gtcgatgacg ccgcggtggt gcagttgtac gcggacggat ttgccgacct gacgctgccg
    13021 gaaaagacct tcgtctggca cctgtacctc gccgcactgg ccgggcgcga catctactac
    13081 gaccagcgct acgctcacag cctcgacatg cgccgcgtgc tcgaggggat cctgacacac
    13141 gggcaggacg tcgagccccg cgtgctcggc gaggtccgcc gatatgcgaa gctgttctgg
    13201 atcaacagcg ggccgcacaa caacctgacg gcgcgcaagt tcgtcctgtc gatcacggcc
    13261 gaggaattgc tcgaggccgc caggtcggcc agcctcgccg gcgcgaggtt gtccgcgaga
    13321 gccgacgaac cggttgacgc gctcatcgag cgtcttgcgc cgttgttcct cgacgactcg
    13381 gtcgatccgt tcgtcacgag caagtcaccc ggtgaggggc gcgacatgct gcaggcgagc
    13441 tcgaacaacc tctactctgg ggtgagcgtc cgcgacctcg attccttcga ggagcgctat
    13501 ccgttgaact cccgtctcaa gggcgagaac ggccgactcg tcgaagaggt ctacagagtg
    13561 ggcggacgct atgaccggga gatccgacgg atcatcggtc acctgcggga cgcgattcca
    13621 tttgccacgc cgccggccgc cgagtctctg cgggcgctca tccgcttcta cgagaccggg
    13681 gaggaggcgg acagaatcgc ctacgacatc gcctgggtcg gcgacagcga atcacccgtg
    13741 gacaccatca acggcttcgt cgaagtctac ctggacccgc gaggcgcgaa gggcgcgtgg
    13801 gagggcatcg tctgctacgt tcacgcggcc aagacacgcg cgctgcagac gttggccgtg
    13861 cacgcgcaat ggttcgagga tcacatgccg tgggacgcga ggttccgcaa gaccgacgtc
    13921 aagggcatca cggtgcgagc catcgaggtg gtcgtcgaga cgggagattc cgggcccgtg
    13981 tcgcccgtcg gcatcaacct gccgaacgac caggcgattc gcgagcggca cgggagcaag
    14041 tcgatgtcgc tgaccaatgt cagcgacgcc tacgagcgct cgactcccga cgacatgaga
    14101 acggaattcg cctggagccc ggacgaggcc gaccgcagcc ggcgttgggg cggcttcgtg
    14161 caggagctca cgacggccat gcacgaggtg atcgggcacg gatccgggcg gatggcgccg
    14221 ggagttgccg actcgccgtc ccgtctgctc gcggagcagt actcggcaat cgaggaggcg
    14281 cgcgcggatc tcgtcgcctt gtacttcctt cccgatgcga agctcgtgac gctcgggctc
    14341 gtgcccgccg gggagcagga cgacattgtg aaggcggaat acgaacacta cgtccgcaac
    14401 gcgctggtgc agcttcgacg cgtgcgcgag gggacgaagc tggaagagga ccacatgcgc
    14461 agccgccaga tgatcgtgaa ctggctgcgc gcacatacca cctcggtcga cgtacgtcgg
    14521 cgagacggca acacgtactt cgtgatgacc gatcgagcgg cgtttcgcga gggcgtcggg
    14581 cgcctgctcg ccgaggtgca gcgcatcaag agcgaagggg accttgccgc cgcacgaggg
    14641 ctgttcgaga cctatggcgt ccacttcgac ccggctctgc gggacgaagt ggtgacacgc
    14701 gtcgatcgcc tgaggcttcc gtcgtacacc gggttcgtca tgccgaggct gcacgcgcgg
    14761 tggaacgagg ccggagcgat cgtggacgtc gagatgtcct acccgtgcga cctcgaggcg
    14821 cagatgctcg agtactctgc cttctcgcgc cacgacgatg ttctccaagc gctttcctcg
    14881 tcacgctgag atcaacgagc tgactcggac cctgcagagg ctgcgggccg agggcgcgtc
    14941 gatcacggac ctgactgact cgaacccgac ccgggttgga gtcacgtatc cggacgacct
    15001 gctggacgac ctgtcgagcg aagccgcgcg tcgctacgaa ccgcattccc tcgggctgcg
    15061 gtccgcgcgc gaggcagtcg cggccgacta cggtcgccgc ggggcccgtg tcgatccaga
    15121 tcacgtcgtg ctgtcggcca gcacgagcga ggcatacagc tggatcttca agctgctgtg
    15181 cgatccgggc caggccgtcc tcgtgccgga gccgagctac ccgctgttcg agcacctgac
    15241 gggcgccgag ggggtgaagg ccgtcgccta ccagcttcgt tatcacggac gctgggagat
    15301 cgacatcgac tcgcttgccg ctgccccgtc ggatacgcga gccgtgctcc tcgtctcacc
    15361 gaacaatccc actggatcgt tcgtgtccag gctggaggcc aatcgcatgg ccgcgctctg
    15421 ccgcgagcgc ggatgggcaa tcgtggccga cgaagtgttc gcggactacg cgctggacga
    15481 agcggcggcc gtcacggaat gcgcgatgaa cctcggggtg ctgtcgctga ccctcggcgg
    15541 cgcgtcgaag acgctcggcc tgccgcaggt gaagctcgca tggatggtcg ttgacggccc
    15601 gccagccgat cgcgaccggg cgctcggcgc gctcgagctc atcgcggaca tgtacttgtc
    15661 ggtcagcacg cccgtgcagg ttgccgcgcc gtcgctgctc gcgcggggcg aggcgatcag
    15721 gcggcagata cgagcgcgcg ttgccgccaa tctggcgcgg gcgcggcagc tgataccgcg
    15781 ctacccggcg tgtacgccgc tgccggtgga aggcggatgg tcgctcgtgg ttcgcgtccc
    15841 gtcgacgcgc tccgaggagg cgctcgtgct ggacctgctc gagcgcgaac gcatcctcgt
    15901 ccaccccggc tacttcttcg acttccctca cgaggcattc attgtcgtca gcctgctgcc
    15961 tccggaggag gtctttgccg acgcgctgga tcgcgctctg tgcgtcgcca ccagcgtcgg
    16021 gggcggccgg ccgtgaatcc gccaggcccg cgccgtgctg gcatcctcgt gccgctgttc
    16081 tcgatcccgt ccggaagaag ctggggcatc ggcgagatcg gggacctcga acggctggtc
    16141 tcgtggctcg cttcggccgg ccaacgtgtg ctgcagctgc tgccgatcac cgaggtatct
    16201 cccggcgacg cgtccccgta cgggtcgctc agtgcaatgg caatcgaccc gcaatacatc
    16261 acgctcgatg cgctggagga cttcgccgcg acgggaggtg aaggcgcact cgcccccgag
    16321 gagcgcgtgg cgttggaccg ggcacgcgcg gcgccggcga tcgaccatcg cctcgtccgg
    16381 gatctgaaac aggcggcgct gcgccgggcc tttgcgcatt tcgtgtcgag ggagtgggcg
    16441 ggtggaacag ccaaggccgc cgccctgcgc gcgttcaccg ctgaacaggc gtggtggctc
    16501 gaggactacg cgctcttccg cgcgctgcac gcacgccatg acgaaaggcc ctggatggag
    16561 tggccggagc cgatccggtc gcgacagccg cacgtgatcg agatcgagca ccttgcgctc
    16621 ggcaaagaga tcctgtttcg tcagtacctg cagtgggtgg cgggggagca gtgggcccac
    16681 gcacgtgcgc acgccagggc ctgcggcgtc gccatcttcg gcgacctccc tttcgtcgtc
    16741 agcgcggaca gcgcggacgt gtgggcgcgg caggacgagt tcctgctcga ggaatcagtt
    16801 ggcgcgccgc cggacgcctt cagcgaaacc ggacaggact ggagccttcc tccgtaccgg
    16861 tgggaggtcg tgacggcacg cgacttttcc tggctgcggg agcgcgtcag gcgctttgcc
    16921 aacctgttcg acggctgcag ggtcgatcac gtcgtgggct tttatcgaac cttcgtgcgt
    16981 ccgccaggcg gcggggccgg ccggttcagt ccagccgacg aggcctcaca gttgtcgctc
    17041 ggcgaacgcg cgctcgccgc gctcacgtcg gcgccgatgc agatcgtggc ggaggacctc
    17101 ggaaccgtgc ccgacttcgt ccgtgagtcg ctggcacggc tgtcgattcc gggctgcaag
    17161 gtgttccgct gggagcgccg gtggaacgaa cccgggcagc cattcaagga ccccatcgag
    17221 taccccgccg cggccatggc gacgtccggg acccacgaca ccgagtcgat ggcggtgtgg
    17281 tgggaacagg cgcccgtcga ggaacggcga gccgtgctgg cgattccttc ggtcagggcc
    17341 cggatgtccg aagacgatcg ggcgagggcc gttgacgcgt ccgccctgtc gccggcgacg
    17401 cgcgcgatcc tgctcgaaat cctctttgct tcccgctcga cgctcctgat cctgccgatc
    17461 caggatgtgt tcggctggtc tgacaggatc aacaggcccg gcaccatcgg ggatcacaac
    17521 tggacgtggc ggctgccgtg gccgagcgac cggatgtccg cccaacccga ggcggcggcc
    17581 gctgcggacc aactacggga gtggtcgagg cgctacgaac gctgactgat cctggcccag
    17641 gtgtccttga gcgtcaccgt ccggttgaat acgggagctc ccggtgccga gtcggggtcg
    17701 acggtgaagt aaccctgccg ttcgaattga aacgtggcac ctgcggatgc gccggcgagc
    17761 atttcttcca ccttgcatcc tgtcagcacg cggagcgagt cggggttgag atcggacagt
    17821 gggtcccgcc caccctctcc cggatcgtcc gatcgaaaga ggcgatcgta caggcgcact
    17881 tcggcatcgg aggcgtgcgc cgccgacacc cagtggatcg ttcccttgac gcggcgtgag
    17941 gcggtctcgc cgttgagcga gccgcggtcg atcgtgcatc gaagctcgat cgggaccccg
    18001 tccgcgtcct tgacgacctg ttcgcacttc aggatgtacg catacctgag ccggacctcg
    18061 ttgcccggcg agaggcggaa gaacttcttc ggcggattct ccatgaagtc ttcgcgctcg
    18121 atgtagagca cccgggaaaa cggaatcgtc cggtgacccg cggaggggtt ctccggattg
    18181 ttgacggcat cgacgtactc gacgtgtcct tcggggacgt tgtcgatttc cacgcggagg
    18241 ggctgcagaa cggccagcgc acgctgcgcg cgccgattga gatcttcgcg cacggtgtgc
    18301 tcgagcagcg ccacgtcgat cacgttctcc ttcttggcca cgccgatgcg cgtgcagaag
    18361 tcacgaacgg cctcgggcgt gtagccgcgc cggcggatgc ccgcgatggt cggcaagcgc
    18421 gggtcgctcc agccgcgaac gagcccctgc tcgacgagcg cgagaagctt gcgcttgctc
    18481 atcaccgtga agttgaggtt caggcgtgcg aactcgatct gctgcggccg cgagcggaac
    18541 gccgcgtcgt gcctgacgtt ctcgacgacc cagtcgtaga gcggccggtg atcctcgaac
    18601 tcgagcgtac acaacgagtg cgtaatgcct tcgagcgcgt cggagagggg atgtgcgtag
    18661 tcgtaggtcg ggtagatgca ccaggcatcg cccgtccggt ggtgagtggc gtgcctgatg
    18721 cgatacagcg tcgggtcgcg catgttgatg ttgggcgacg ccatgtcgat cttcgcgcgc
    18781 agcacgtggg taccgtcggg gaactctcct gcgcgcatgc ggcgaaagag atccaggttc
    18841 tcctcgatgc gccgcgcgcg atacgggctg tctttgcccg gagcggtcag cgatccccgg
    18901 tactcgcgaa tctggtcggc cgagaggctg tccacgtagg ccttctcgtc ctgaatcagc
    18961 tgctcggcgt agagaaacat ctgctcgaag tagtccgagg cgtagaacat cctcgagctc
    19021 cagtcgaagc cgagccaccg cacgtcggcc tggatcgagt cgacgtactc cacgtcttcc
    19081 ttcgtggggt tggtgtcatc gaagcgcagg ttgcagaggc cgccgaactc cgcggcgacg
    19141 ccgaagttga ggcagatcga cttggcgtgc ccgatgtgca ggtatccgtt gggctcgggc
    19201 gggaaccgcg tgtggacacg gctggcgtga cggcccgctc gaagatcctc ggcgatgatg
    19261 tcgcgaatga agtcctggga gcgtgcaggg tccacagctg tcggcggttc gggcatctgg
    19321 gaagaacgat tctaccggct accggctacc ggcgaccagc tagtggagcg tatcagcgaa
    19381 acgtaaagcg ggccgcccgt tggctcccac cccagcgcgc aaacaacgcg cgccggggac
    19441 cccgggttgg cccgcgaaat cggcgtcgaa gggctgtccg agtcgctaca ggttcagttg
    19501 atacagtcca ctagtcgtcg tcagtcaggg ctccctcggg cagccccatg agtccggctt
    19561 gcttgaggac gcggggcacg cgttcgatcg gatcggccgg gggaagctcg ggcgcagtcg
    19621 tcacgtccag atcgcgcagc cgccgggcgc tgacgagcac gcgcgattcg aaggaccccg
    19681 cggcctcgtt gtacgcctgt accgctccgt ccaatcgctt gcgcacatcg tcgaagcgat
    19741 ccgcgaaggt ggcgagccgt tcgtagaact ctttaccgag ccgggccact tcccgatagt
    19801 tctccgtcag cgcctcgtgc cgccacgagt gtgcgaccgt cgtcagcagc gcgatgagcg
    19861 tgatcgggct tgccagcagg acgcgctgct ccagcccgtg ttcgatcagc gtcagatcct
    19921 gctgcagcgc cgcgccgaac agcgtttcgc ccggcaggaa catcacgacc atctcgggag
    19981 agttggggag ctcgtcccaa tacgccttgc tccccagccg gtccatgtgc tcccggacct
    20041 gccgcgcgtg tccctgcagc cgcagcgcgc gctgctcgtc gtcggtgcac tcctgcgcct
    20101 cgagaaatgc ttcgaggggc gtcttcgcgt cgacgacgat ctgcttgttt cccggaaggt
    20161 ggacgacgag atcgggacgg aaccggccgt tgtcgctcgc aagcccgggc tgttcgtcga
    20221 agtcgcactg gtgcagcatg cccgagatct cgacgacccg ccgcagctgc atttcgcccc
    20281 agcgcccccg gacggccggc gtccgaaggg ccctcgacag cgtgcttgcc gtggatccga
    20341 gtgcgatgac ctgctccgag agtctcgagt aggcttcgac acgctcgcgt tcagcggtgt
    20401 cgagccgctg atccacgcgc ctcagcgtgt cggcaatcgg ctggcggtac ccctcgaacg
    20461 atgtcttcgc gagatcgaag aacgatgcgc ggttgtcctt cagggcgtcg gcggcaagag
    20521 attgaaacgt gtcgcgcaag cgctgctcgg cccggtcgac gaggctcttg gccgactcga
    20581 gcgcctcttt ctgccgcccg gcgtcctcgc gaacccactt gagatcctgg cgcgcggcgg
    20641 ccagctccgc ggcggcggca gcgcgagcgt cggcaacctg acgcaaggcg acgctccgca
    20701 tgacggcgaa gaccacgagc gcggtcacgg cggacgcgat cagccactca ctcaaggctc
    20761 aatgctcacg gttcgatgat tacacaaggc gccagaaaaa catccgattc gtaattgcca
    20821 gcagaactgc actgcgggtt cacgaattcc ggcccgaatc cgcgcagcga gagcggcgcg
    20881 gtcgttgcag ccgcgtccgt cgtggtttcc cgagtcgcgc acgaagcgcc gtcgatcagg
    20941 caggacgggt tcggcgtccg ccagatcgag gacgttccac gagccgggag ggtcgagcgc
    21001 cttgccgtgt cgcccgcact cgcgacgccc acgtccgaac aggcgatccg ggcccgcgcc
    21061 gctcatctga acaggctcgg cccgggaccg atcggccgag tcgccaagat cgagcgaaag
    21121 gcgtcgggac tgatcatcac gagcctgttg cccgacggcg tgccgttgtc ggacatcctc
    21181 gccgcactcg agttcggcac gctgaccttg tccgacgacg aagtgctcga gctggcggcg
    21241 tcggtcgttc gcggagcggc caggatgcac gagatgctcg gttccctcgc gcatggcgcg
    21301 ctgacgcctg cgcacgtggt gctgatgcgt gacggcacgt cggtgttcac cggagccgtg
    21361 ttcggggacg cgattcagtc gctgcagcga aaccgcgaga gcatctggcg cgaattcggc
    21421 ctcgcgcttc cagcggcggc ggccgccccg cggttcgaca agcggagcga cgtcacccag
    21481 ctgggcgcga tcgtgctggc gatcgccctg cgcaggagcc tgcgtcgcga cgagttcccg
    21541 cgtgggatcg gcgatctcat catgttgacc acggccgcct cgacgttcga cggcaactcc
    21601 aggctgcaca tgtggctgca ggacgggctg cagctgcacg ggcgcgtcgt gttcgactcg
    21661 gccgtggacg cctcgcgcgc cttcgaccgc ctgctgcccg acgactgcgg cgacggggcg
    21721 gaagccctgg ctcttcgaac ggcgatcctg cagatgtccg gcgcatcgtc ctcgggcgcc
    21781 tctgccgctt gagggaggac gccggtcccg cgctccagcg gccagcttcc gcacacgcaa
    21841 ccctgcttga taccatcgct gattgacgcg gcgggccccg acgcgcccgc ccggattggt
    21901 gatgggtcga ctgacgattg ctctcgcgtt gatgccatgg ctcgcggcgt gtggcggcca
    21961 ggcgccgcct gcccctgtcc tgcccgacgt ctacctcgcg cccgatacga ccattgcgcg
    22021 aggcgtcgtt gctcccggca gcacgctcga gacgatgctc ctcgaccagg gactcgagac
    22081 cggcgtcgtg cacggggtga tcgacgccgt gcggatggtg ttcgatcctc gccggctccg
    22141 ttctctccag ccgttcctgc tggaattgtc tctggacggc gcgctgcggc tcttcgagta
    22201 cgagatcgac gccgcgtcct tcctccgcgt cgggcccggg cgtgatgccg cgaacgcgct
    22261 tcacgcagag gtcgtgccga ttccctcgac tctcgtccac gaatcggttc gtggcgccat
    22321 cgacgacgcc acgccatcgc tgttccagtc ggttgaagcc gtcggtgaac agcccgacct
    22381 cgccattgcc atggcgcaga tcttctcggg cgagatcgac ttcaccagcg agctgcagcc
    22441 tggcgaccgc tatgccatcg ccttcgagcg attcgagcgc gatggtcgtc cctcgtccta
    22501 cggcgcaatc acggcagccg agttccgcaa cgagggccgt gtgattcgag ccatccgctt
    22561 cacaccggcc ggcggcgagc ctgggtatta cgacgaagag gggcgatcgc tgcgccgatt
    22621 cttcctgaag tcgccgctca aattcgagcc gcgcgtcacc tccggcttct cgtcgcggag
    22681 gatgcacccg gtcctgcacc aggcgcgcgc gcatcgcggc gtggactacg gtgcgccgcg
    22741 gggcgccgag gtcgtgtcgg tggcgtccgg ccgggtgctc tctgccagtt tcgacaacgc
    22801 caacggccgc atggtccgca tcaggcacac gtcgggatac gtctctatgt atctgcactt
    22861 gtcgtccttc gcctccggag tccgcgcggg ggctgccgtc gcgcaggggc agacgatcgg
    22921 ccgcgtcgga tcgacgggcc tcgcaaccgg tccgcacctc cattacggac tgcagaagaa
    22981 cggcgtgttc gtgaaccctc ttcgcgagca ccggaacatg ccgccgggcg agccggtccc
    23041 gagcgccgat cgtgccgcgt tcaacgtcga acgcgaccgg gcgcagtcgc tgttctcgca
    23101 atagctctcg tctccgtatg gccgcactcg ttcctttccg ggcgctacgg ccggccgcga
    23161 cgtcggcggc gcgcgtcgcc gccgttccct acgacgtcgt gaacaccagc gaggcgcgcg
    23221 cgctcgcatc ggacaacccg ctcagctttc tccatgtgtc ccgagcggag atcgaccttc
    23281 ccgatggcgc cagcccgtac gccgacgcgg tgtacgcgcg ggctgcggcg aatttcgatc
    23341 ggctgcggca gcaggccccg ctcgtcatgg aagacgcgcc gcgcctgtac gtctacaggc
    23401 tgcggatggg ccgccattcc cagacgggcc ttgccgcctg cttttcggtg gacgagtacg
    23461 aacgccgcgt catcctcagg cacgagcaca cgcggccgga caaggaggac gaccgcacgc
    23521 gacatctgct gacgctcggc gcccagactg gccccgtctt cctgacctat cgtgcgaggc
    23581 cggcggtgga tggcctcgtg gagcgcgtga cgacgacgac gccggtgtac gacttcaccg
    23641 cgatcgatgg cgtgcagcac acgatctggc tcgtcggcgc ggcggaggag cgccagatca
    23701 tcctcgcgtt cgacgcgatt ccggcactct acatcgccga cggacatcac cgcgccgcca
    23761 gcgccgcccg cgcgaggcgg acgctcgccg gggcggccca cgccacgggc gagtgggact
    23821 ggtttctcgc cgtcgcgttc ccggattcgg aggttcagat tctgccgtac aaccgcgtga
    23881 tcgcggacct ggctggcatg actcccgccg cgtttctgga cgcgttgcgc gctcgcgccc
    23941 tcgtccagtc cggtcccgcc gagccgcagc gccgcggcga ggtcgcgatg ttcctccagg
    24001 gttcgtggca cacgatcgtc cttcccgacg cgccggcagc tgtgtcgcct gccgatcggc
    24061 tcgatgtgac acgcctgcag gcgaccgtgc tcgagccgtt gctgcggatt ggggacgttc
    24121 gcaccgacaa gcgcatcgac ttcgtcgggg gcgtacgcgg gacggccgag ctcgaggccc
    24181 gtgtgcgatc aggtgtcgcg gccctcgcct tctcgatgtt tccagttgac gccgccgacc
    24241 tgatggcgat tgccgatgcc gggggcatca tgccgcccaa gtccacgtgg ttcgagccga
    24301 agctccgaga cgggctgctg tcgcatgtga ttagctagga ccatgacaac gaccacgcgt
    24361 gtgttcaatt tctccgccgg accggccgtc ctgccgctgc cggtgctcga gcaggcgcag
    24421 cgcgagctcg tggcgctccc cggcgtcggc atgtccgtca tggaaatcag tcaccggtcc
    24481 cggaccttcg aggacatgct gcagtcgacg atcgcgggca tccgcgatct ggccggtgtt
    24541 ccgtcgaact acaagatcct gttcctgcag ggtggcgcga gcctgcagtt cacgatggtg
    24601 ccgatgaacc tgctgcgcgc cggga
//
LOCUS       DBNGSH010000232         6500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3753807, whole genome shotgun sequence.
ACCESSION   DBNGSH010000232 DBNGSH010000000
VERSION     DBNGSH010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3753807"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="AC16CY_05375"
     CDS             <1..1619
                     /locus_tag="AC16CY_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIR3353245.1"
                     /translation="FRPGQEDLLRAVLHGRDVLAVMPTGSGKSLGFQLPALLLPGTTL
                     VVSPLISLMKDQVDELNRRGIPAAAIHSQVAAGARQQAVTAARRGELRLLYVAPERFA
                     SESFVRALQDLAVARFIVDEAHCVSEWGHDFRPDYRRLKSAAASCRRDDDRPGRVPIA
                     AFTATATPEVRDDIVELLGLADPSVVVAGFDRPNIHLRVRPVANELEKIELLPGLVSD
                     RRALVDAATRRNAETSADALRAGGVEAAAYHAGLNEAERTRVQDAFAGGTLRVVCATN
                     AFGMGIDRPDVDVVVHLDIPGSLEAYYQEIGRAGRDGRPAVATLLWHYADVKTREFLI
                     DKEPEDRPGRPAVAIDPADVARRKDIEHKKLRRMVAYADAAGCLRATILRYFGDPSAR
                     EPCGSCGTCDRRAPLDEDARLLVRKILSGIARAGERYGRRKIAAMLVGNVDDLPESLT
                     KLSTTGLLRHEEPRTVERWIDAAAAAGLIRASDDQYRTLGLTPVGRDVMAGRLADVQM
                     AVPSIRSWVSRRKATRSPGAKRRPRRRGGS"
     gene            complement(1633..2619)
                     /locus_tag="AC16CY_05380"
     CDS             complement(1633..2619)
                     /locus_tag="AC16CY_05380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3353246.1"
                     /translation="MNARAAALATAVVLAGDVFAQDAQAPNPDAFYQLGPDSLEQPGV
                     PKGEIRGPFTLPSAAYPGTQHTYWVYVPAQYRAAEPASLMVFNDGQAFKNPTGNIRAH
                     NVLDNLIHRREIPVMLAVFINPGRRPDQPEPTPQNWGDRDTNRPTEYNSLDDRYARVI
                     VDELLPALAKDYNISPDPDRRGIGGASSGAIAAFTVAWERPTAFRKVLSIVGSFVNLR
                     GGHAYADIVLKAEKKPLRVFLQDGRNDNRALSQTGTYDQTRDWFFQNVRLMEALTKMG
                     YDVNYTWGMNRHGQAMGGAILPEMMRWLWRDQPVSTDPRDMVERSLRGAEPR"
     gene            2752..5922
                     /locus_tag="AC16CY_05385"
     CDS             2752..5922
                     /locus_tag="AC16CY_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353247.1"
                     /translation="MKILYLGNDTESIAAALRGIHDITVLEGSHLDQAGKWLAENPDI
                     AALVVEAQLDGGDWRSVLTPLGALAVRPAVVVVVPEGTGPLFESLEPGADGYIEKGQS
                     LVRELQGAITRAVAPARGRDLEQKLARATAALQDAERRHQAAMAEATAQIAQRQTQYE
                     IAVARASAIWEMVDEQLRAAALEVERARQDQASAVAEADRLTRRDTDLSSRLAAAEEA
                     VEAAIARADQERAAAADHLAERQRELQAEIEQESAHRRRVEDTLAHAMRTLEDAEKRH
                     ESAMSGAAAHSRELEQALRLTERDLESTLADVERLTTRAVELSSTLADAATRRDDLEH
                     RLAATEAAVRDGDTERLVASRRAAAREAELDGQIRTEREARTALEQGLADADAALRAA
                     QQRHQTALAAAANELAEREAHLDRELSRTAADRDRLAQQLSDTEATLDQARRDHHSAA
                     INVERLTEVEADLVSQLGAARAAQHTLERRLADAVREAADADERAARDRAAATEREAT
                     LESQFAEERLAYEGRLDEAGQRHRTLTLERDALQESLAASQEHSRRLDEELHEARGQF
                     DQAQASADADIRRLTAECFETERELQESRRHSQETFDRLSSEHATALAALAASVTGRD
                     EQLREQSEVKETLIQELEAAKRRLQEVQAHLQTVQTEADEVPRLRTQVEASREESHRL
                     FQQAGLAMFRCSRDGALIEANRACETLIGRRMIDELRGAQFAPAVFEAPDVLSWLIER
                     CVSTTAKESADTTWRRQDGRRLFVRLSASVVATDLIEIAAEDLTRIRILQERLDKAHR
                     MEAVGRLASEVAATCGNLLDDIHQHAQKWLTTAGTDNASRQHGEALLEDVSRAVGLLR
                     QLAACGEEQARAPRLVDLNTIIRDLEPVLKRVAGGDVDVRLQETSSPLNVDVGMERVE
                     RLLVNLASYGRERMPFGGRLAIELGTVVVDRHFAVKHPDVRLGLHALITVTETRRAAG
                     ADGPPRHQATARHSQGSVEPRAGVDFRTLQQLVSECGGHLWVKVQPPGDMVVKIRLPL
                     FSGSHDETGPNARGAGVRAIVRRFRP"
     gene            5969..>6500
                     /locus_tag="AC16CY_05390"
     CDS             5969..>6500
                     /locus_tag="AC16CY_05390"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR3353248.1"
                     /translation="MYSPRQSSFDATRHLWIALAAAVSFNTACRTAPAPPPVSPDTWA
                     VVGGKEITRSEVEKAFRRMDTGAQQPVSDEEALAAKLSLLDELIVQEILLAKATALKI
                     EVADTEVDAAFNEGRKNLTPAAFEQELARRQLTEGDMREGVRRELLTRKLLEREVVSK
                     VTVTDQQVTDYFNANRS"
BASE COUNT         1081 a         2202 c         2285 g          932 t
ORIGIN      
        1 cgtttcgccc cgggcaggag gatctgctgc gtgccgtgct ccacggccgg gacgtgctgg
       61 ccgtaatgcc gaccggatcg ggcaaatcgc tcggctttca gttgccggcg ctgctgctgc
      121 caggcacgac gctcgtggtc tcgccgctga tctcgctgat gaaggatcag gtcgacgagc
      181 tcaaccgccg cgggatcccg gcggcagcga tccattcgca ggtcgccgcc ggcgcgcgcc
      241 agcaggccgt gaccgcggcc cggcgcggtg agctccgcct cttgtacgtg gcgcccgagc
      301 ggttcgcctc ggagtctttc gttcgcgcgc tgcaggatct ggctgtcgca cggttcatcg
      361 tggacgaggc gcactgcgtg tcggaatggg gccacgactt ccggcccgac taccggcggc
      421 tgaagagcgc ggccgcctcg tgccggcgag acgacgaccg gccggggcgc gtgcccatcg
      481 ccgcgttcac cgcgacggcc acgccggagg tccgcgacga catcgtcgag ctgctgggcc
      541 tggccgatcc cagcgtcgtc gtggccgggt tcgaccggcc caacatccac ctgcgcgtcc
      601 ggccggtggc caacgaactg gagaagatcg agctgctgcc gggactggtg tccgatcgcc
      661 gcgcgctggt ggacgccgcc accagacgca acgcagagac gagcgcagac gcgctgcgcg
      721 ctggcggcgt cgaggccgct gcctatcacg ccggcttgaa cgaggccgag cgaacgcgcg
      781 tgcaggacgc attcgccggc ggcacactgc gtgtcgtgtg cgccaccaat gccttcggca
      841 tgggcatcga tcgccccgat gtcgacgtcg tggtgcatct cgatattccc ggctcgctcg
      901 aggcctacta ccaggagatc ggccgtgccg gccgcgacgg gcgcccggcc gtggccacgc
      961 tgctctggca ctacgccgat gtcaagacgc gcgagttcct catcgacaag gagcccgagg
     1021 atcggcccgg ccggccggcg gtcgcgatcg atccggccga cgtggcccgg cggaaggaca
     1081 tcgagcacaa gaagctgcgc cgcatggtgg cctacgccga cgccgccggc tgtctgcggg
     1141 ccacgatcct tcggtacttc ggcgatccct cggcgcgaga gccgtgcggg tcgtgcggca
     1201 cgtgcgaccg gcgcgcgccg ctcgacgagg acgctcggct cctggtccgc aagatcctgt
     1261 cgggcatcgc gcgggccggc gagcggtacg gacgccggaa gatcgcggcc atgctcgtcg
     1321 gcaacgtgga cgacctgccc gagtcgctca cgaagctgtc gacgacgggc ctgctgcgtc
     1381 acgaggagcc gcggacggtc gaacgctgga tcgatgcggc ggctgccgca gggttgatcc
     1441 gtgcctccga tgatcagtac cgcacgctcg gcctgacgcc ggttggccgc gacgtgatgg
     1501 ccggccgcct cgcggacgtg cagatggccg taccttctat ccgctcctgg gtgagtcgtc
     1561 gtaaagccac tcgcagccca ggcgccaagc ggcgcccgag gcggcgagga ggatcgtagc
     1621 gcccggaaac agctagcgag gctccgcgcc gcgcaacgac cgttcgacca tgtcgcgcgg
     1681 gtcggtcgag accggttgat cgcgccagag ccagcgcatc atctccggca ggatcgcccc
     1741 gcccatcgcc tggccgtggc ggttcatgcc ccaggtgtag ttgacgtcgt accccatttt
     1801 cgtcagcgcc tccatgagcc gcacgttctg gaagaaccaa tcacgcgtct ggtcgtacgt
     1861 cccggtctgc gaaagcgctc gattgtcgtt cctgccgtcc tgcaggaaga cgcgaagcgg
     1921 cttcttctcc gccttcagca cgatgtccgc gtaggcgtgg ccgccgcgca ggttgacgaa
     1981 gctgccgacg atgctgagga ccttgcggaa ggcggtgggg cgctcccacg cgacggtgaa
     2041 ggcggcaatg gcgccagagc tcgcgccgcc gatgccgcgg cgatcgggat ctggcgaaat
     2101 gttgtagtcc ttcgcaagtg ccggtagcag ctcgtcaacg atgacgcggg cgtagcgatc
     2161 gtcgagcgag ttgtactcgg tcggccggtt cgtgtcgcgg tccccccagt tctgcggcgt
     2221 cggctccggc tgatcgggcc gcctgcccgg gttgatgaag acggccagca tcacggggat
     2281 ctcgcgccgg tggatcaggt tgtcgagcac gttgtgcgcg cggatgttgc cggtcgggtt
     2341 cttgaacgcc tgcccgtcgt tgaagaccat caggctcgcc ggttcggcgg ccctgtactg
     2401 cgcgggcacg tacacccagt aggtatgctg cgtgcccgga tatgcggcgg agggcagcgt
     2461 gaacgggccg cggatctcgc ccttcggaac ccccggctgc tcgagcgaat ccggtccgag
     2521 ctgatagaag gcgtcgggat ttggcgcctg cgcgtcctgc gcgaagacgt ccccggccag
     2581 gacgacggcc gttgcgagcg ccgcggcgcg agcgttcaca cgcggatgat cgtgcggacc
     2641 cgccggggcg tcaagagctc agcgttagga ggcgcctgga aaccccgtgc agtagaagga
     2701 tcatcgggcc gatagaagga cacgaaccgg tccggcgcgg catcagggca catgaagatt
     2761 ctctacctcg ggaacgacac agagtccatc gcggcggctt tgcgaggcat tcacgacatt
     2821 acggtcctgg agggttctca cctcgatcag gccgggaaat ggctcgcgga gaaccccgac
     2881 attgccgcac tggtcgtgga agcccagctc gacggcgggg actggcgctc ggttctgaca
     2941 cccctcggtg cactcgccgt gcgtcccgct gttgtcgtgg tcgttccgga gggaaccggg
     3001 cccctgttcg agtcactcga gccgggcgcc gacggctaca tcgagaaggg gcagtcgctc
     3061 gtccgcgagc tgcaaggcgc gatcacccgt gcggtggccc ccgcacgcgg gcgggacctc
     3121 gagcagaagc tcgcgcgggc caccgccgca ctgcaggatg cggagcggcg ccatcaggcg
     3181 gccatggcgg aggcgaccgc ccagatcgcc cagcgccaga cccagtacga gatcgccgtc
     3241 gcgcgggcgt cggccatctg ggagatggtc gacgagcaac tgcgcgcggc ggccctcgaa
     3301 gtagaacgcg cgcggcagga tcaggcgtcg gccgtcgccg aggccgatcg actgacccgc
     3361 cgcgacacgg acctttcgtc acggctggcc gcagccgaag aggccgttga agccgccatc
     3421 gcgcgcgccg atcaggaacg ggccgcggcc gcggaccacc tggccgaacg acagcgcgag
     3481 ctgcaggccg agatcgaaca ggaaagcgcc caccggcgcc gcgtcgaaga cacgcttgcg
     3541 cacgcgatgc gtacgctgga agacgccgag aagcggcacg aatcggccat gagcggcgcg
     3601 gccgcgcact cccgtgaact ggaacaggcc ctccggctga cggagcggga cctcgagtcg
     3661 accctggccg acgtcgagcg gctcaccacg cgtgcagtcg aattgagctc gacgctcgcc
     3721 gacgccgcga cgcgccgtga cgatctcgaa catcgattgg ccgcgacgga agcggccgtt
     3781 cgtgacggcg acaccgaacg gctggtggca agcagacggg ccgctgcacg cgaggcggag
     3841 ctcgatggac agattcggac ggagcgcgag gcgcgaacgg ccctggagca gggccttgcg
     3901 gatgccgacg ctgcgctgcg ggccgcgcaa cagcggcacc agacggctct cgccgcggcg
     3961 gccaacgagc tcgccgagcg cgaggcgcac ctcgatcgtg agttgtcgag gaccgcggcg
     4021 gatcgcgaca ggctcgccca gcagttgagc gatacagagg ccacactcga tcaggcacgc
     4081 cgcgaccatc actcggctgc catcaacgtg gagcgcctca cggaagtcga agccgatctc
     4141 gtctcccagc tcggcgccgc ccgcgccgcc caacacacgc tcgagcgccg gctggccgat
     4201 gccgtgcgcg aggctgcgga cgccgacgag cgtgccgccc gcgaccgggc cgccgcgacc
     4261 gaacgcgagg cgacactcga gagccaattc gccgaggagc gactggcata cgaaggtcga
     4321 ctggacgaag ccgggcaacg ccaccgcacg ctgacgctgg agcgcgatgc gctccaggag
     4381 tcgctggctg caagccagga gcactcacga cgcctcgatg aggaactcca cgaggcgcgc
     4441 ggacagttcg accaggcgca agcgtcggca gacgccgaca ttcgccggct caccgccgaa
     4501 tgctttgaaa ccgagcgcga gctccaggag tctcgccgtc actcccagga aacgttcgac
     4561 aggctttcaa gcgagcatgc caccgcgctg gccgccctcg ctgcctcagt gaccggacga
     4621 gacgaacagc tgcgggagca gtcggaggtc aaggagacgc ttatccagga gctcgaggcc
     4681 gcgaagcgcc gcctgcagga ggtgcaggcg cacctccaga ctgtgcagac ggaagccgac
     4741 gaggtgcctc gactccggac gcaggtggag gcgagtcgcg aggagagtca ccggctgttc
     4801 cagcaggctg gactggcgat gttccggtgc tcgagagacg gcgcgctcat cgaggccaac
     4861 cgcgcgtgtg agaccctgat cggccgccgc atgatcgacg aactgcgggg tgcgcagttc
     4921 gcgcctgccg tcttcgaggc gccagacgtc ctgtcctggc tcatcgagcg ctgcgtgagc
     4981 acgacggcga aggagtcggc cgacaccacg tggcggcggc aggacgggcg ccgcctcttc
     5041 gtccgtctgt cggcgtccgt cgtggccacc gatctcatcg agatcgccgc cgaggatctg
     5101 actcgtattc gcattctcca ggagagactc gacaaggccc atcgaatgga agccgtcggg
     5161 cggctcgcct ccgaggtggc ggccacgtgc ggcaatctgc tcgacgacat ccatcagcat
     5221 gcgcagaagt ggctgaccac ggccggcacc gacaacgcgt cgcgacagca cggtgaagcg
     5281 ctgctcgagg acgtgtcacg ggcggttggc ctcctgcggc aactggccgc gtgcggcgag
     5341 gaacaggccc gcgcgccgag gctggtcgac ttgaacacga tcatccggga tctcgagccc
     5401 gttctgaagc gggtcgccgg aggcgacgtc gacgtccggc tgcaggagac gtcgtctccg
     5461 ctgaacgtcg atgtcggaat ggaacgggtc gagcgcctcc tcgtcaatct ggccagctac
     5521 ggacgggagc gcatgccctt cggtggccgg ctggcaatcg agctcggcac cgtcgtcgtc
     5581 gatcgccact tcgccgtcaa gcaccccgat gtcaggctcg ggctccacgc gctgatcacc
     5641 gtcaccgaaa ccagacgcgc cgcgggtgcc gatggaccgc ctcgccacca ggcgaccgcc
     5701 cgccactcgc aggggagcgt cgagccgagg gcgggcgtcg acttcaggac gctccaacag
     5761 ctcgtcagtg agtgcggggg ccacctgtgg gtgaaggtcc aaccgcccgg cgacatggtt
     5821 gtgaagatcc gcctgcccct gttctcgggc tcgcacgacg agacaggccc gaatgcccgc
     5881 ggcgctggcg tccgggcgat cgtccgccgg ttcagaccct aagtccccca gttgttcctg
     5941 gcacgcgtcg cgagtacgct gccagagcat gtacagtccc cgacaatcct cattcgatgc
     6001 cacgcgccac ctctggattg cgctggccgc cgctgtctct ttcaacacgg cctgtcggac
     6061 ggcgccggcg ccaccgcccg tctcgcccga tacgtgggcc gtggtcgggg ggaaggaaat
     6121 cacccgcagc gaagtcgaga aggccttccg gcggatggat accggcgcgc agcagccggt
     6181 gtctgacgag gaggcgctgg cggccaagct gagcctcctg gacgagctca tcgtgcagga
     6241 gattctgctc gccaaggcga ccgcgctgaa gatcgaggtg gccgacacag aggtggacgc
     6301 cgccttcaac gaggggagga agaacctcac gccggcggcc ttcgagcagg aactggcgcg
     6361 acgacaactg acggaagggg acatgcgaga aggcgtccgg cgcgagctgc tgacgcggaa
     6421 gctgctcgaa cgggaggtcg tctcgaaggt caccgtcacg gatcagcagg tcacggatta
     6481 cttcaacgcc aatcgctcgc
//
LOCUS       DBNGSH010000233         4820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4093708, whole genome shotgun sequence.
ACCESSION   DBNGSH010000233 DBNGSH010000000
VERSION     DBNGSH010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4093708"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="AC16CY_05395"
     CDS             complement(<1..294)
                     /locus_tag="AC16CY_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3353249.1"
                     /translation="MIALTHRLDRTLVVRAARDTVFQYFTDTPRWASWWGAGSTIDAR
                     PGGRMVIRHANGVEVSGEVLEVSPPERIVFTYGYANQPQIPPGSSRVTIRLDSH"
     gene            complement(291..668)
                     /locus_tag="AC16CY_05400"
     CDS             complement(291..668)
                     /locus_tag="AC16CY_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3353250.1"
                     /translation="MDTALLSTLASPRRREILRLLWTRDLTAGEVHQAMPDVTFGAVS
                     LQLKVLLEAGLVECQADRQYRVYRVRREALGPVASMLEEMWTDALARLRLKAELEAAR
                     RGPRPRRRRSPRSRRSRTKPEDT"
     gene            complement(823..1881)
                     /locus_tag="AC16CY_05405"
     CDS             complement(823..1881)
                     /locus_tag="AC16CY_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353251.1"
                     /translation="MKAALIVLAVASSALVEAQWLNQPTAGIPRTPDGKPNLSAPAPR
                     TPDGRPDLSGLWTKISPRYSRNIAADLKPDEIQPWARALVEERRENLGKDYMNVRCVP
                     LGPGYATAADSTGSEMIKIVQTPGLIVILNPDLTYRQIFLDGRALEPDPNPTWMGYSV
                     GRWEGDTLVVESFGFNDRTWLDHDGHPHTEALRVTERYRRRNFGHLDVEVTMSDPKAY
                     SRPWTVAVRAEIAADTEMIEWVCNEGRSGLEHWVGTASDERKGEVEVAPGILSGYVGT
                     YEEQPPFWRTVPRTVRITLSNGRLVANMDGRGDVALAASSPTTFSGLYGLGVEFIGGA
                     SGGLFVKHVSGNYRFAKQ"
     gene            1996..3453
                     /locus_tag="AC16CY_05410"
     CDS             1996..3453
                     /locus_tag="AC16CY_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HIR3353252.1"
                     /translation="MLAQEPLVNPPFVSIEDAARAIAAQASADASQTTGDQRQGVPLP
                     DPRRACDGCPPRRLGSAFLQTTVVNVIYWSGNMIPGKELAQVTPATWWNNMQRGWNWD
                     LDDFSINQFGHPYQGNSYFTTGRANGLNFWESSALTAFGSGTWEYFGETGQASFNDFI
                     NTTLGGIALGEMFHRAAWLVRDTRATGKPRRMREIAAMVIDPMTGFNRFASGDANRVV
                     DKPPEMVPTALSSVTSVGALWRDSTADGVLSTVDPLIEVTLLYGDLEKGVSRTPFDAF
                     AVRLDFGGGNFISEARVRGRLFGQPFQKGGVQLNVAQGYQYLKNSAYQFGAQSVELHA
                     GLSRQLTSRFSFWANGWGGLTILGAVDSLPLGGVPVTESPPSSSEDGHSTEPRFYDYG
                     PGTNFGGFIDVRRDGRTFFTFAYDAHHIYVLDGVRANHLLQRLRADLMVPIRGRLGIA
                     AAGEFFDRQTFYQDPLNSRARYHYPQFRLSLTWGS"
     gene            3456..4073
                     /locus_tag="AC16CY_05415"
     CDS             3456..4073
                     /locus_tag="AC16CY_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353253.1"
                     /translation="MARRLASWALLPLCLLPALAHAQAPAPAAAAQDPALPSRLWVVV
                     GGGSTSLLGDCTDCAAEDHIHSGNFMLNAGVSLNRRTDVGAEILYVPTTLETGEPISV
                     SFVMAAVQFRPWQTRGFFLKAGSGLASLRNWLGTIDGVDTAQRSTAFALGLGTGWEWR
                     MRGRFGAQVFGSQHVVALGDLETNTGIAENVMGNFWSFGAAIVIR"
     gene            4109..4510
                     /locus_tag="AC16CY_05420"
     CDS             4109..4510
                     /locus_tag="AC16CY_05420"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3353254.1"
                     /translation="MTSRVPGLVLAVGLAGASASAHHSIAGVYDGSQRVTIEGTVAEF
                     QFVNPHPFVIVDVADGNAPPRRWRLELDNRHELVAEGMNADTLKPGDRVLAIGSPGRT
                     LPHTMYVRRLDRRSDGFRYEQVGSSPRVRRP"
     gene            complement(4533..>4820)
                     /locus_tag="AC16CY_05425"
     CDS             complement(4533..>4820)
                     /locus_tag="AC16CY_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3353255.1"
                     /translation="ETGARPDGTFIALDPVRQHSGKTVYAWAVESEFDPAALRSNLFE
                     MEWPPRSGRRQSFPEVDRAEWFTMAVAGRKILSGQAPLLRQLEERVRITGL"
BASE COUNT          759 a         1588 c         1623 g          850 t
ORIGIN      
        1 gtggctgtcg agccgaatcg tcacgcggga gcttcccggc gggatctgcg gctggttggc
       61 gtacccgtag gtgaagacga tgcgctcggg tgggctgacc tcgagcactt ctcccgacac
      121 ttcgacgccg ttcgcatgcc ggatcaccat ccgcccgccc gggcgagcgt cgatcgtcga
      181 gcccgcgccc caccacgacg cccagcgcgg cgtgtcggtg aagtactgaa acacggtgtc
      241 gcgcgccgcg cggacgacga gtgtgcgatc gagacgatgg gtgagtgcga tcatgtgtcc
      301 tccggcttcg tgcgagagcg tctggagcgc ggggaccggc ggcgccgcgg ccgcggcccg
      361 cgccgggctg cctcgagctc ggccttgagc ctgaggcgcg cgagcgcgtc ggtccacatc
      421 tcctccagca tcgaggccac ggggccgagc gcctcgcgtc gaacgcggta tacgcggtat
      481 tggcggtcgg cctggcactc gacgaggccc gcctcgagca gcaccttgag ctgcagcgac
      541 acggcgccga acgtcacgtc cggcatcgcc tggtggactt cacccgcggt gaggtcccgc
      601 gtccacagca gccgcagaat ctcgcggcgg cgaggggagg cgagggtgga aaggagcgcg
      661 gtgtccatgc cgacgccgta agtttagata aaactaaaat acaagtcaag tccgccgccc
      721 acggacgtta accacagagt cgcagagaac acggagttga tgtttcccag gaaaacctct
      781 gtgcgctctg cgtctctgtg gttagcggtc gtggcggcga gtctattgtt ttgcgaagcg
      841 gtagttgccc gatacgtgct tcacgaagag gccgccggat gcacccccga tgaactcgac
      901 cccgagtccg tagaggcccg agaacgtggt gggagacgag gcggcgagcg ccacgtctcc
      961 ccggccatcc atgttggcca ccaggcgccc gttcgagagc gtgatgcgga ccgttcgggg
     1021 gacggtcctc cagaagggag gttgttcctc gtaggtcccg acgtagcccg acagaatccc
     1081 gggggccact tcgacttcgc ctttccgttc gtccgaggcg gtgccgaccc agtgctccag
     1141 gccacttcgg ccctcgttgc acacccactc gatcatctcg gtgtcggcgg cgatctcggc
     1201 ccgcaccgcg accgtccacg gcctcgagta cgccttcgga tccgacatcg tcacctcgac
     1261 gtcgagatgg ccgaagttcc gccgccggta acgttcggtc acgcgcagcg cttcggtgtg
     1321 aggatggccg tcgtggtcga gccacgtgcg atcgttgaag ccgaagctct cgacaaccag
     1381 cgtgtcgccc tcccagcggc caaccgagta ccccatccac gtgggattcg gatcgggctc
     1441 gagcgcgcgg ccgtcgagga agatctgacg gtaggtgaga tcgggattga ggatgacgat
     1501 caagccaggc gtctgcacga tcttgatcat ttccgagccg gtgctgtcgg cggcggtggc
     1561 gtatccaggt ccgagcggca cgcagcggac gttcatgtag tccttgccga gattctcgcg
     1621 gcgttcttcg accagcgccc gcgcccaggg ctgaatctcg tccggcttca ggtcggcggc
     1681 gatgttgcgt gagtacctcg gcgagatctt ggtccacagg cccgacagat cgggtcttcc
     1741 gtccggcgtg cgcggcgcgg gagccgagag gttcggtttg ccatccggcg tgcgcggaat
     1801 tcccgcagtc ggctggttca accactgcgc ctcgaccagt gcgctcgacg cgacggccag
     1861 gacgatgagc gcggctttca cggcagtcag ggggattgtg gcacgaagcc atcggtgccg
     1921 tctgctaaca tagccgttcc cgtggttcgg gcccttttcc cccccgttct ggctcttgtg
     1981 cttggtgcga ctgaagtgct tgcacaggag ccgctggtca atccgccgtt cgtctcgatc
     2041 gaggatgcgg cgcgtgcaat agcggcgcag gcctccgccg atgcgagcca gacaactgga
     2101 gatcagaggc agggcgtccc gttgccggac cctcggcgcg cgtgcgacgg ctgcccgccc
     2161 cgccgcctcg gctccgcgtt tctgcagacg acggtcgtca acgtcatcta ctggtcgggc
     2221 aacatgatcc cgggaaagga gctcgcgcag gtcacgcccg cgacgtggtg gaacaacatg
     2281 cagcgcggct ggaactggga cctcgacgac ttctccatca accagttcgg ccatccctat
     2341 cagggaaaca gctacttcac gaccggacgg gccaacggcc tcaacttctg ggaatcgtcg
     2401 gcgctgacgg cgttcggcag cggaacctgg gagtacttcg gcgagaccgg ccaggcgtcg
     2461 ttcaacgatt tcatcaacac cacgctcggc gggatcgcgc tcggcgagat gttccaccgc
     2521 gcggcctggc tcgtgcgcga cacgcgcgcc accggcaagc cgcgcaggat gcgcgagatt
     2581 gccgccatgg tgatcgaccc gatgaccggc ttcaaccgct ttgcatccgg tgacgccaat
     2641 cgcgtggtcg acaagccgcc cgagatggtg ccgacggcgc tgtcgtcggt cacgtcggtc
     2701 ggcgcgctct ggcgcgactc gaccgccgac ggcgtcttgt ccaccgtcga tccgctgatc
     2761 gaggtgacac tgttgtacgg ggacctggag aagggtgtca gccgcacgcc gttcgacgcg
     2821 tttgccgtgc gcctcgactt cggcggcggc aacttcatca gcgaagcgcg cgtccgcgga
     2881 cggctgttcg ggcagccctt tcagaagggc ggcgtgcagt tgaacgtggc ccagggctat
     2941 cagtatctga agaacagcgc ctatcagttc ggcgcgcagt ccgtcgagct gcacgcgggc
     3001 ctttcacgac aattgacctc ccgcttctcg ttctgggcga acggctgggg aggcctcacc
     3061 atcctgggcg cggttgactc gttgccgctc ggaggagtcc ccgtaacgga gtcgccgccc
     3121 tcgtcctccg aggacggcca ctcgacggag cctcgtttct atgactacgg cccgggaacg
     3181 aactttggcg gattcatcga cgtgcgccgc gatggccgca cgttcttcac gtttgcgtac
     3241 gacgcgcacc acatttacgt gctggacggc gttcgcgcca accacctgct ccagcgcctc
     3301 agggcggacc tgatggtgcc cattcgaggc cggctcggga tcgccgcggc gggagagttc
     3361 ttcgatcgcc agacgttcta ccaggatcca ctgaacagtc gcgcgaggta tcactatccg
     3421 cagttcaggt tgtcgctgac gtggggctcg tagggatggc acgacggctg gcgtcgtggg
     3481 cgctcttgcc gctctgcctg ctgccggcgc tcgctcacgc acaggccccg gctccggccg
     3541 ccgccgccca ggatcccgcg ctgccgtcgc ggctgtgggt cgtcgtcggc ggtggctcga
     3601 catcgctcct cggtgattgc acggactgcg ccgcggagga ccacatccat tcaggcaatt
     3661 tcatgctgaa cgccggcgtg tcgctcaatc gccggaccga cgtcggagct gaaatccttt
     3721 acgtcccgac cacgctcgaa acgggcgagc cgatcagcgt gtcgttcgtc atggccgcgg
     3781 tccaatttcg gccgtggcag acccgcgggt tcttcctgaa ggccgggtcg ggactggcct
     3841 ccctgcgcaa ctggctcggg acgatcgatg gcgtcgacac ggcccagcga tcgacggcct
     3901 ttgcgctcgg gctcgggact gggtgggaat ggcgcatgcg cggccggttc ggcgcacagg
     3961 tgttcggctc gcagcacgtg gtggcgctcg gagacctcga gaccaacacg ggcatcgccg
     4021 agaatgtcat gggcaacttc tggtccttcg gcgccgcgat cgtgattcgg tagtacgggg
     4081 agtcgcgtga aacgcgcata atcgtcccat gacgagccgg gtccccggac tggtgctggc
     4141 ggtggggctc gccggcgcct cggcgtctgc ccatcactcg atcgcgggcg tctacgatgg
     4201 cagccagcgg gtgaccatcg aaggaacggt cgccgagttc cagttcgtca atccgcatcc
     4261 gttcgtgatc gtggatgtcg ccgacgggaa cgcgccgccg cgacggtggc gcctcgagct
     4321 cgacaatcgt cacgagctcg tcgccgaggg catgaacgcc gacacgctga aacccggcga
     4381 tcgcgtgctg gcgatcggca gcccgggccg cacgctgcct cacacgatgt acgtccgccg
     4441 tctcgatcgc cggtcggacg gcttccgcta tgagcaggtg ggaagcagcc cgcgcgttcg
     4501 tcggccctga aaaatggggt cgggagtcat tttcagagtc cggtgatcct gacgcgttct
     4561 tcgagctgtc gcagcagcgg cgcctggccg ctcaggatct tccgcccggc gaccgccatc
     4621 gtgaaccact cggcacgatc gacttcaggg aacgactggc gccggcccga gcggggcggc
     4681 cattccatct cgaagagatt gctccgcagg gccgcgggat cgaactcgct ctcgacggcc
     4741 cacgcgtaga ccgtcttgcc ggagtgctgc cgcacggggt cgagagcgat gaacgtgcca
     4801 tcgggccgtg ccccggtctc
//
LOCUS       DBNGSH010000234         2029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2225535, whole genome shotgun sequence.
ACCESSION   DBNGSH010000234 DBNGSH010000000
VERSION     DBNGSH010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2225535"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /locus_tag="AC16CY_05430"
     CDS             complement(<1..1725)
                     /locus_tag="AC16CY_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3353256.1"
                     /translation="MRLWPGVAAVAVLLVSLFAIPFAPPSTTTIAFIVLYVSALLIVV
                     WWLAFSRARWYERIGALAAMALAVAVMRPLLDQSIIGGAMGNLTYVLAFPLLSLALVA
                     WAVGTRRHSVPVRLATLPTAMLLASGLLTLLRTDGMGGETMLQLRWRWMPTAEERLLA
                     EITDVPVAPIAPSPSPSAPPAAEVPAEPAATRSEDEPAPSTPASPVPIRAAEWPGFRG
                     PDRNSVVRGVRINTDWVASPPVELWRRPIGPGWSSFAVDGDRIYTQEQRGDDEIVACY
                     SLATGKPVWMHKDATRFWESNAGPGPRGTPTLVNGRVYAFGATGILNALDARTGAVVW
                     SRNAASETKVETPTWGFSSSPLVTGDLVIVAIAGTLAGYDLASGKPRWVGPKHAFSYS
                     SPHLVTIGDVAQVLLLSPPGVVSVTPSDGTLLWEHAFEGGGIVQPTVTPDGDVLISGL
                     AGTGGSGVRRLGVARGSDGWTAQERWTSNGLKPYFNDYVVHNGNAYGFDGSILASIDL
                     ADGKRRWKGGRYGAGQLILLPEQDLLLVVSEEGGLALVGATPDKFTEIGRAPALDGKT
                     WNHPVLVGD"
BASE COUNT          338 a          700 c          683 g          308 t
ORIGIN      
        1 gtcgccgacc agcacggggt ggttccaggt cttgccgtcg agtgcgggcg cgcgtccgat
       61 ctcggtgaac ttgtccgggg tggcgccgac cagcgccagc ccgccttctt ccgacaccac
      121 cagcagcaga tcctgctcgg gcagcaggat cagctgcccc gcgccgtagc ggccgccctt
      181 ccacctgcgc ttgccgtccg cgagatcgat gctcgcgagg atgctgccgt cgaatccgta
      241 cgcgttgccg ttgtgcacga cgtagtcgtt gaagtacggc ttgaggccgt tcgaggtcca
      301 gcgctcctga gccgtccatc cgtcggatcc gcgcgcaacc cccagccgcc tgacgccgga
      361 gccgccagtg cctgcgaggc cgctgatcag gacgtcgcca tccggcgtca ccgtcggctg
      421 cacgatcccg cccccctcga aggcgtgctc ccacagaagc gtgccatccg acggcgtgac
      481 gctgacgaca cccggcgggc tcaggaggag cacctgcgcg acatcgccga tggtcaccag
      541 gtggggcgag ctgtagctga acgcgtgctt gggacccacc cagcgcggct tgccgctggc
      601 gaggtcgtag ccggcgagcg tgccggcaat cgccacgatg accaggtcgc ccgtcaccag
      661 gggtgagctc gagaagcccc acgtcggggt ttccaccttg gtctctgacg ccgcgttgcg
      721 cgaccagacg acggcgcccg tccgcgcatc gagcgcgttc aggatcccgg tcgcgccgaa
      781 tgcgtacacg cggccgttga cgagcgtcgg cgttccgcgc gggccgggcc cggcgttcga
      841 ttcccagaac cgggtcgcgt ccttgtgcat ccataccggc ttgccagtcg cgaggctgta
      901 gcacgccacg atctcgtcgt cgccgcgctg ctcctgcgtg tagatgcgat cgccgtccac
      961 ggcaaaggac gaccatcctg gtccgatcgg gcgccgccac agctcgaccg gcggcgacgc
     1021 cacccagtcc gtgttgatcc ggacgccgcg gaccacgctg tttcgatcag gaccgcggaa
     1081 gccgggccac tcggcggcgc gaattggcac agggctcgcc ggcgtggacg gggcgggttc
     1141 gtcctccgac ctggtcgcag ccggctcggc cggaacctcc gcggccggcg gagcggacgg
     1201 cgaaggggac ggcgcgatcg gcgcgaccgg tacgtcggtt atctccgcga gcaaccgctc
     1261 ctcggccgtc ggcatccacc gccacctcag ctgaagcatc gtctcgccgc ccatgccgtc
     1321 agtgcggagc agcgtcaaca atccagacgc caggagcatt gccgtaggaa gcgtcgcgag
     1381 ccgaacgggg acggaatgcc ggcgagtccc gaccgcccac gcgacgagcg cgagcgacag
     1441 cagcgggaat gcgaggacgt aggtcaggtt tcccatcgcg ccgccgatga tcgactggtc
     1501 gagtagcggc cgcatgacgg ccactgcgag agccatcgcg gcgagcgcgc caatccgttc
     1561 gtaccatcgc gcgcggctga aggccagcca ccagacgacg atcagcaggg cgctgacgta
     1621 caagacgatg aacgcgatcg tcgtggttga agggggggcg aacgggatgg cgaacaacga
     1681 aacgagcaga accgccacgg cggcgacgcc cggccagagt ctcatcggtt gtcgcatgat
     1741 gcccccgtca gaccagcgcg ctcgagacgt cgagctgtcg atagtctagt ggtatgtcct
     1801 tcgcggatta ttgccggccg ctccgttgtc gcggccggaa aatcgcggtc gaagctcgtt
     1861 gttcgcggta ctcttccagt tgatacggtc cacaaagtgt acgattgtcg ataatcgagg
     1921 tataggtgtc aaccatgcgc cgcctcacgc cagaaacgcg agcgcgaagt tcgccagcat
     1981 cgcgatcgga tacagccccc ggccccacgt gtcaacgcgg gcgatgagg
//
LOCUS       DBNGSH010000235         4455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_867208, whole genome shotgun sequence.
ACCESSION   DBNGSH010000235 DBNGSH010000000
VERSION     DBNGSH010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_867208"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="hemW"
                     /locus_tag="AC16CY_05435"
     CDS             <1..1013
                     /gene="hemW"
                     /locus_tag="AC16CY_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR3353257.1"
                     /translation="HAKADGAAADTVYFGGGTPSLLEPREIGRLVEACRQSFALSDDA
                     EITLEANPETVTAERLQAFRDAGVNRLSFGVQSFRDQELQRLSRLHSAARAREAFGMA
                     RAAGYQNVSLDLMMWLPQQAVADWLLSVDALIDLDPEHASLYLLEIYPNAPLRDEMAR
                     SRWSVAPDDDAAEMYLQAMARLDAAGYTQYEISNVSRPGRQSRHNLKYWTDQEWLGFG
                     CGAHSTRKGAREKNIASISEYNAAVKAGRSLVAERRTLCNQDRFEEAVFTGLRLTQGI
                     RLGEVESRYGIDVWARYGGELQPFVDQRLLIYDDGALRLTREGMLLANEVMAVFIRPS
                     VR"
     gene            1045..1503
                     /locus_tag="AC16CY_05440"
     CDS             1045..1503
                     /locus_tag="AC16CY_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353258.1"
                     /translation="MRRLSRVLLAGAILVLPAFSHSASAQAAAPQKFTMDGELAILTV
                     AVNADKTADYEKILGRVKDVLSKSTAADDKQQIAGWKVLKMAKPLPDGKIAYMHVITP
                     VPGADYAILQILYRSITDPTEQKALYDTYRAAFAGNLSLASGPVVVDMSK"
     gene            1604..2125
                     /locus_tag="AC16CY_05445"
     CDS             1604..2125
                     /locus_tag="AC16CY_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353259.1"
                     /translation="MRVVPAVILTALATAAAGAAQQAPAPAPAPAKPDPTTPVSSVRT
                     FTAPVGLLLNAVRPERVADFEKLIGHLQAALAASSDATIREQAKGWKVYKASEMGPNG
                     SVLYVFLLDPTVKGADYGLGRILAEAFPDTARLQEIWKLYNDSIASGGSLVNLTPVDP
                     AAGSQQPGAGGKK"
     gene            complement(2142..2618)
                     /locus_tag="AC16CY_05450"
     CDS             complement(2142..2618)
                     /locus_tag="AC16CY_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIR3353260.1"
                     /translation="MRTIRVALASIALLAGASPAFADATAFLGTASSPSNRLTRGFAV
                     GFGLVIVGFEFEYGDTVEDPADSAPSLRTGMANVYLQTPGAIFGLQPYVTTGAGVYHE
                     SLGTASQTNFGLNSGGGVKISIAGPLRARIDYRVFSLRDSPLDPVHRFYVGVNLRF"
     gene            2660..3805
                     /locus_tag="AC16CY_05455"
     CDS             2660..3805
                     /locus_tag="AC16CY_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3353261.1"
                     /translation="MNVCLTDEQELLRRSVREFAEAELRPHVREWDEAQELPREVLGR
                     LAGLGLMGIQIPEQYGGAAMSAVDYCICIEELARVDPSVCLSVAAHNGLGVAHLFMFG
                     TEEQKRTYLVPLAAGRQLAAWALTESVSGSDAAAMRTTATRDGDDWVLNGSKQFITHG
                     RGGDVVVVLAVTSRGKGSRGISAFIVERGTPGLRAGRKEDKLGMRASETSEVILEQCR
                     VPARQMLGAEGQGFVHALQVLDAGRIGIAALAVGLAQGAYEAARTYAFERKQFGQPIG
                     AFQSIRSKLVDAAYRIEGARLLTYRAAVMKDEGRRTTLEAAMAKLYSSEIAVRVSEDG
                     VQIHGGYGFVKDYPAEKYFRDVKLTTIGEGTSEIQRLVIARQLLATL"
     gene            3870..>4455
                     /gene="meaB"
                     /locus_tag="AC16CY_05460"
     CDS             3870..>4455
                     /gene="meaB"
                     /locus_tag="AC16CY_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR3353262.1"
                     /translation="MTPDPIYALAARVLDGDPRAIARAISLVEEESAAGQDLVRRIYG
                     RTGRAYLVGVTGAPGTGKSTLVDRLIALIRRAGLKVGVIAVDPTSPFTGGAVLGDRVR
                     MGAHAGDRGVFIRSMATRGHLGGLARATADAALVLDAAGMDVVLIETVGVGQDEIEIV
                     STADVSIVLLVPGTGDDLQAIKAGVMEIGDIFVVN"
BASE COUNT          783 a         1351 c         1574 g          747 t
ORIGIN      
        1 ttcacgcgaa agcggacggg gcggcggccg acacggtcta tttcggcggc ggaacgccgt
       61 ctctgctcga gccgcgcgaa atcgggcgtc tcgtcgaggc gtgccggcag tcgttcgcgc
      121 tgtctgacga tgccgagatc acgctggagg cgaacccgga gacggttacg gcggagcggc
      181 tgcaggcgtt tcgagacgcc ggcgtcaatc ggctcagttt cggtgtccaa tcttttcgag
      241 accaggagct tcagcgtttg agccgcctgc actcggcggc gcgggcgcgg gaggcgttcg
      301 ggatggcgcg cgcggccgga taccagaacg tgtcattgga tctgatgatg tggctacccc
      361 aacaggcggt agcggactgg ctgttatctg tcgacgcgtt gatcgatctg gacccggagc
      421 acgcgtcgct gtatctgctg gagatctacc cgaacgctcc cttgcgcgac gagatggcgc
      481 gttcccgctg gtccgtggca cccgacgatg acgcggccga gatgtatctg caggcgatgg
      541 cgcggctcga tgcggccgga tacacgcaat acgagatctc gaatgtctcg cggccaggcc
      601 ggcagtcgcg gcacaatctg aagtactgga ccgaccagga gtggctcggc ttcggctgcg
      661 gtgcgcactc gactcgaaaa ggcgcccggg agaagaacat tgcctccatc tccgagtaca
      721 acgcggccgt gaaggctggc cggtccctcg tggcggaacg gcgtacgttg tgcaatcagg
      781 atcggttcga ggaggcggtg ttcaccggcc tgcggctgac ccaggggatc aggctcgggg
      841 aggtggaatc gcggtacggg attgacgtct gggcgcgata cggcggggag ctgcagccgt
      901 tcgtggacca gcgactgttg atctatgatg acggcgccct gcgcctgacc agggaaggaa
      961 tgctgctggc gaacgaggtc atggcagtgt tcatacggcc atcggtacgt taggattggc
     1021 agccaattcc agggagggtt gaagatgcga cgactgtcca gagtcctgtt ggctggcgcg
     1081 attctcgtct tgccggcatt ttcgcattcc gcgtccgcac aggcggcggc gccgcagaag
     1141 ttcacgatgg acggtgagtt ggcaatactt accgtcgccg tcaacgcgga caagacggcg
     1201 gactacgaaa aaatccttgg ccgcgtcaag gacgtgctca gcaaatcgac ggcggccgac
     1261 gacaagcagc agatcgcggg gtggaaggtt ctgaagatgg ccaagccgct gccggacggc
     1321 aagatcgcct acatgcacgt catcactccc gtcccgggcg ctgactacgc tatcctccag
     1381 atcctttaca gatcgatcac cgatccgacg gagcagaagg cgctgtacga cacgtatcga
     1441 gccgcttttg ccggcaacct cagtctagcg agcggtcccg tcgtcgtcga catgtcgaag
     1501 tagccgttgt cgcggcgcga ctcagacgcc cgcgcagcct acaatgaaga gccacggacg
     1561 gcctcgagcc gctcgtggct tttgtctttg tggaggcaac ccgatgcgtg tggttccagc
     1621 cgtcatcctg acagcgctgg cgacggcggc ggccggcgca gcgcagcagg cacccgcgcc
     1681 tgcgcccgcc ccggctaaac ccgacccgac gacgcctgtg tcctccgtgc ggacgttcac
     1741 cgcgcccgta ggtctgctcc tgaacgccgt tcgccccgag cgcgtggccg acttcgagaa
     1801 gctcattggc catctgcagg cagcgctcgc cgcatccagc gacgcgacga tccgggagca
     1861 ggcgaaaggg tggaaggtgt acaaggcgtc ggaaatgggg ccgaacggat cggtgttgta
     1921 tgtattcctg ctcgatccca ccgtaaaggg agccgactac gggctgggcc gcattctcgc
     1981 ggaggcgttt cccgacacgg cgcgcctgca ggagatctgg aagctctata acgactcgat
     2041 tgcgagcggc ggcagccttg tgaacctgac gccggtggat ccggcagccg gcagccagca
     2101 gccgggggcc ggcgggaaga agtaggcagc cggcagcgcg ctcagaatct caggttcacg
     2161 ccgacgtaga agcggtgcac gggatcgagc ggcgagtctc gcagcgagaa gacgcggtag
     2221 tcgatccgcg cccgcagagg gccggcgatt gaaatcttga cgccgcctcc actgttcaag
     2281 ccgaagttgg tttgcgacgc cgttccgagg ctttcgtggt acacgccggc gcccgtcgtg
     2341 acgtacggct gcaggccgaa gattgcgccg ggcgtctgca ggtacacgtt tgccatgccc
     2401 gtacgcagag acggtgcact gtccgccggg tcttcgaccg tgtccccgta ctcgaactcg
     2461 aagccgacaa tgacgaggcc gaagccgacg gcgaagcctc gggtcaagcg attcgagggc
     2521 gacgaggcgg tgccgagaaa cgccgtggcg tctgcgaacg cgggcgacgc gccggcgaga
     2581 agcgcgatgc tcgcgagagc gactctgatg gtgcgcattc tcgaatgata cgatcggttc
     2641 cgatgagaga tgtcagccga tgaacgtctg tctcaccgac gagcaggaac tgctgcgtcg
     2701 cagtgtgcgg gagttcgcag aggcggagct tcgcccgcac gtcagggagt gggacgaggc
     2761 gcaggaactc ccgcgcgagg tcctcggcag gctcgccggg ctcggcctca tgggcattca
     2821 gattccagaa cagtacggcg gggcagcgat gtcggcggtg gactactgca tctgcatcga
     2881 ggaactggcc cgcgtcgatc cgtcggtgtg tctgtcggtg gcggctcaca acggtctggg
     2941 ggtggcgcat ctcttcatgt tcggaacgga ggagcagaag cgcacctacc tggtcccgct
     3001 cgcggcgggc aggcagctgg ccgcgtgggc gctgaccgag tcggtgtccg ggagcgacgc
     3061 tgccgccatg cgcacgaccg ccacgcgcga cggcgacgac tgggtgctca acggatcgaa
     3121 gcagttcatc acgcacggtc gcggcggcga cgtcgtggtc gtgctcgccg tcacgagccg
     3181 cgggaagggc agccgcggca tctcggcctt catcgtcgaa cgaggcacgc cggggcttcg
     3241 cgccggcagg aaggaggaca agctcgggat gcgggcgagc gagacgagcg aagtcatcct
     3301 ggagcaatgc cgcgtccccg cccggcagat gctcggggcc gagggccagg ggttcgtgca
     3361 cgccctgcaa gtgctcgatg ccggccgcat cgggatcgcc gcgctggcgg tgggcctggc
     3421 acaaggcgcc tacgaggctg ctcgtactta cgcgttcgag aggaaacagt ttggacaacc
     3481 gatcggcgcg ttccagagca tccggtcgaa gctcgtggac gcggcgtacc ggattgaagg
     3541 ggcgcggctg ctgacgtatc gtgccgccgt catgaaggac gagggtcgcc gtacgacgct
     3601 cgaagcggcg atggcgaagc tgtactcgag cgaaatagcc gtgcgggtca gcgaggacgg
     3661 cgtacagatt cacggcggct acggcttcgt gaaggactac ccggccgaga agtatttccg
     3721 cgacgtcaag ctcacgacca tcggcgaagg cacgagcgag attcagcggc tcgtgatcgc
     3781 gcggcaactg cttgcaactt tgtaatgggg tcgggagtca ttttattgac gacgctcgtt
     3841 acacgttggt cgcggagcgt cctcaataaa tgactcccga ccccatttac gcgctcgctg
     3901 cgcgggtgct cgacggcgat ccgcgcgcga tcgctcgtgc gatttcgctc gtcgaggagg
     3961 agagtgccgc gggccaggac ctcgttcgcc ggatctacgg acggaccggc cgtgcctacc
     4021 tggtgggcgt caccggggcg cccgggacgg ggaagagcac gctcgtcgat cggctcattg
     4081 ccctgattcg ccgcgcgggg ctgaaagtcg gcgtcatcgc ggtcgatccc accagccctt
     4141 tcacgggcgg cgccgtgctc ggcgatcgcg tgcgcatggg cgctcatgcc ggcgaccgcg
     4201 gcgtcttcat ccgcagcatg gcgacgcggg gacacctcgg cggcctggcc cgggcgaccg
     4261 ccgacgccgc gctggtgctc gacgccgccg gcatggacgt agtgctgatc gagacggtgg
     4321 gcgtcggtca ggacgagatc gaaatcgtct ccacggctga cgtgtcgatt gtgctcctcg
     4381 tgcctggtac gggagacgat ctccaggcga tcaaggcggg agtcatggag atcggcgaca
     4441 tcttcgtggt gaaca
//
LOCUS       DBNGSH010000236         2449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1036883, whole genome shotgun sequence.
ACCESSION   DBNGSH010000236 DBNGSH010000000
VERSION     DBNGSH010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1036883"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /gene="carB"
                     /locus_tag="AC16CY_05465"
     CDS             <1..2025
                     /gene="carB"
                     /locus_tag="AC16CY_05465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR3353263.1"
                     /translation="FPGRSENLDEEDESSLRQKLVVPSDRRMWAVFRALVRGWDVAAI
                     HDLTKIDPWFLRQFADIVELRKMADLGEFREMSVDLLRTLKRSGFADADIAAVYGIDE
                     SLVRQRRIDEGLVPAYKRIDTCAAEFESFTPYMYGTYEQVDESDPTPRKKVVILGSGP
                     NRIGQGIEFDYCCCHAVAGFGQEGFETVMVNCNPETVSTDYDTADRLYFEPLTFEDVM
                     AVIERERTAGGDVACVVQFGGQTPLKLALPLLEAGVTILGTSPDSIDLAEDRQRFSQL
                     LRDLGITQPQSGTAVTREEAREVAERIGYPVVVRPSYVLGGRGMAIVFDSGALDSYMT
                     GAVDVSHDRPVLIDRFLEDAYEFDVDAVADADGAVVIGGIMEHIEEAGIHSGDSSCVV
                     PPFMVAERHVATMRDYTRRIARALKVVGLMNIQYAIKDDTVYVLEVNPRSSRTVPYIS
                     KATGVPIAKIAARVAAGRTLAALGLTEDLTAAGVFVKSPVFPFVRFPGVDTILGPEMK
                     STGEVMGGADNFGSAFGKAMMGAGQRLPEKGNVFISVNNQDKPSVLPVARDLSQLGFT
                     LAATRGTAAFLRAHGLEVEVMYKVNEGRPNVADHLVNRSIDMVINTPLGRESFFDDRA
                     VRRAATMAQVPCITTLTGASAAVSAIKALRQQTLTVRSLQDYYSRVETGA"
     gene            2129..>2449
                     /locus_tag="AC16CY_05470"
     CDS             2129..>2449
                     /locus_tag="AC16CY_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353264.1"
                     /translation="MAFVIFVANVVSLVAMIVVCIVASTAAQSFRGRIDIVQVTVTVR
                     DADGRLVTGLTKDDFQIFEDGREETVTQFTDERVPVSLGVLLDASDSMRGQAIDDARV
                     AVDRF"
BASE COUNT          416 a          781 c          850 g          402 t
ORIGIN      
        1 ttcccgggcc ggtccgaaaa cctcgacgag gaggatgagt cgtccctgcg gcagaagctg
       61 gtggtgccga gcgatcgccg gatgtgggcg gtcttccgcg cgctggttcg aggctgggac
      121 gtcgcggcga ttcacgacct gacgaagatc gacccgtggt tcctgcggca gttcgccgac
      181 atcgtcgagt tgcggaagat ggccgatctc ggcgagttcc gggagatgtc ggtggacctt
      241 ctgcggacgc tgaagcgcag cgggttcgcc gacgcggaca tcgccgccgt gtacgggatc
      301 gacgagagcc tcgtccggca gcgccggatc gacgaaggtc tcgtgccggc ctacaaacgg
      361 atcgacacgt gcgccgccga gttcgagtcg ttcactccgt acatgtacgg cacctacgaa
      421 caggtggacg aatcggaccc gacgccgcgg aagaaagtcg tcatccttgg cagcggcccg
      481 aaccggatcg gtcagggcat cgagttcgac tactgctgct gtcacgccgt cgccgggttc
      541 ggccaggaag ggttcgagac ggtgatggtc aattgcaacc ccgagaccgt ctcgactgac
      601 tacgacacgg ccgaccggct gtacttcgag ccgttgacct tcgaggacgt catggccgtg
      661 atcgaacgcg agcgcaccgc ggggggagac gttgcctgcg tcgtgcagtt cggcgggcag
      721 acgccgctca agctcgcgct gccgctgctc gaggcagggg tgaccatcct cggcacctct
      781 cccgattcga tcgacctggc cgaagaccgc cagcggttct cgcagttgct gcgcgacctc
      841 ggcatcacgc agccgcagag cggcacggcg gtcacccgcg aggaggcgcg cgaggtggcc
      901 gagcgcatcg ggtacccggt cgtcgtccgg ccgtcctacg tgctcggcgg gcgcggcatg
      961 gcgatcgtgt tcgattccgg tgccctcgac tcgtacatga cgggcgcggt ggacgtcagc
     1021 cacgatcgtc cggtgttgat cgatcgcttc ctcgaggacg cctacgagtt cgacgtggac
     1081 gcggtggccg acgccgacgg cgcggtggtc attggcggca tcatggagca catcgaggaa
     1141 gcgggcattc attcgggcga cagctcctgc gtcgtgcccc cgttcatggt ggcggagcgg
     1201 cacgtggcga cgatgcggga ctacacccgc cgcatcgcgc gcgcgttgaa ggtcgtgggg
     1261 ctgatgaaca ttcagtacgc gatcaaggac gacaccgtct acgtgctcga ggtcaatccg
     1321 cgttcctcgc gaacggttcc ctacatctcg aaggccacgg gcgtgccgat cgccaagatt
     1381 gccgcccgcg tggcggccgg ccgcacgctg gcggcgctgg gactgaccga ggacctgact
     1441 gccgccggcg tgttcgtcaa gagcccggtc ttcccgttcg tccgttttcc cggcgtcgat
     1501 accatcctgg gccccgagat gaagtccacc ggcgaggtca tgggcggcgc cgacaatttc
     1561 gggtcggctt tcggcaaggc gatgatgggc gcagggcagc ggctgcccga gaagggcaac
     1621 gtgttcatca gcgtgaacaa ccaggacaag ccgtcagtgc tgcccgtcgc gcgcgacctg
     1681 tcgcaactcg gcttcacgct ggcggccacg cgcggcacgg ctgcgttcct gcgcgctcac
     1741 gggctcgagg tcgaggtcat gtacaaggtg aacgagggcc ggccgaacgt cgcggaccac
     1801 ctcgtgaacc gcagcatcga catggtgatc aacacgccgc tcggacgcga gtcgttcttc
     1861 gacgatcgcg ccgtccgccg ggcggcgacg atggcacagg tgccctgcat cacgacgctc
     1921 acgggcgcgt cggcggccgt cagcgcgatc aaggctctgc ggcagcagac gctcacggtg
     1981 cgttcactgc aggactacta cagcagggtc gagacaggcg cctagccacg aaggtcacga
     2041 agggttggcc acgaaggcac gaagatcgca aggggttggc cacgaaggca cgaagaacac
     2101 gaaggttctc gaagcacgaa cagccatcgt ggccttcgtg atcttcgtgg ccaacgtcgt
     2161 gtccctcgtg gcgatgatcg tcgtgtgcat cgtggcctct acggccgcgc agtccttccg
     2221 cgggcgcatc gatatcgtcc aggtgacggt cacggtgagg gacgccgacg gccggctggt
     2281 gaccggcctc acgaaggacg atttccagat cttcgaggac ggcagggagg agaccgtcac
     2341 gcagttcacc gacgagcgcg tcccagtgag cctgggcgtg ctcctcgacg ccagcgacag
     2401 catgcgcgga caggcgatcg acgatgcgcg cgtcgccgtc gatcgcttc
//
LOCUS       DBNGSH010000237         3325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3584105, whole genome shotgun sequence.
ACCESSION   DBNGSH010000237 DBNGSH010000000
VERSION     DBNGSH010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3584105"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2591
                     /locus_tag="AC16CY_05475"
     CDS             <1..2591
                     /locus_tag="AC16CY_05475"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR3353265.1"
                     /translation="FLATYTQLEAYWKRLSRESDRIRLVDIGPTEEGRRQWMAVISAP
                     ENLEQLDRYRDISRRLALAEGLTEAQARQLAADGKAVVWIDGGLHATEVLGAQQLIET
                     VYQLASGMDAETLRFLRDVIVLAVHANPDGHELVANWYMREKDPARRTLAGVPRAYQK
                     YVGHDNNRDFYMSTQAETINMNRVLYREWFPQIVYDHHQAGPAGTVMFAPPFRGPFNY
                     VLDPLVPVTTDFIGAAMHARFAAEGKPGVTMRGGGNYSMWWNGGLRTTAYFHNQIGLL
                     TETIGNPTPIEIPFVPERQVPSADLPYPVAPQRWRFRQSIEYSLTANRAVIDTASRHR
                     EMLLLRAYQMGRNSIERGNRDSWTVTPKRLALAGGSRDRLRDPALRDPRGYILPSDQP
                     DFLTAAKFVSALLESGVAVHRATSPFSTGGSRVPQGSFVVKSAQPFRPHVLDMFEPQD
                     HPDDVPYPGGPPTPPYDNAGWTLAFQMGVKFVRVLDAFDGPLEKIDAAPASPERLAPP
                     GRVIGAAGVAGFLASHHQNDAFVAVNRLLAAGEEVYWPRDRTAGGLPGGTGVMYIPAR
                     SATRAVLERLAADRGLTFTGVATAPAGEALKLRPVRIGLWDRYGGSSPSGWTRWLLER
                     YGFSFDVVYAQSLDAGDLASRFDVIVLTDDAVMDTRRGSGAPPSDRLPPEFKGTTGEL
                     TTARTLPQLKRFVEEGGTLVAIGGAAAIGQALGLPVSSALVDDAARPLARERFYVPGS
                     VLRVSVDNTTPLGYGFEREVDVVFDNSPAFRLGADAPARGLRRVAWFSSAAPLRSGWA
                     LGQRYLENGVAVIDAPLGRGRALLFGPEITFRAQSHGTFKFLFNAIYYGKALPVSSVG
                     GSARAQ"
     gene            2655..>3325
                     /locus_tag="AC16CY_05480"
     CDS             2655..>3325
                     /locus_tag="AC16CY_05480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3353266.1"
                     /translation="MRERSTDVLVIGAGPAGAATALSLAARGVETVLVDRSEFPRDKV
                     CGDALIPDALAALRTLGLEQAVCRRAAVMEEIRVYAPSGCFVSFSGRCACLPRRGLDH
                     LILQAAVQAGASLLTPYSAAPMGRDRVSGAVLRHRDSGDEVRLSARLTVLATGAGIDG
                     ITAFDVVERRQPSAAAARAYFKVPAGLAQSMKHLVFSYDASTAPGYGWIFPGPDDVFN
                     VGVVT"
BASE COUNT          502 a         1119 c         1156 g          548 t
ORIGIN      
        1 actttctggc gacgtacacg cagctcgagg cgtactggaa gcgcctcagc cgggaatcgg
       61 atcggattcg actggtggac atcggtccga ccgaagaggg ccggcggcag tggatggccg
      121 tcatctcggc gcccgagaac ctcgagcagc tcgatcgcta tcgcgacatc tcgcgccgcc
      181 tcgcgctggc agaggggctg accgaggcac aggcccgcca gctcgccgcc gacggcaagg
      241 ccgtcgtctg gatcgacggc ggcctgcatg ccacggaggt ccttggcgcg caacagctca
      301 tcgaaacggt gtatcagctc gccagcggta tggacgccga gacgctgcgc ttcctgcgcg
      361 acgtcatcgt gctcgccgtg cacgccaacc cggacggcca cgagctggtg gccaactggt
      421 acatgcgcga gaaggatccg gcgcggcgga ctctggccgg cgtcccgcgc gcgtaccaga
      481 aatacgtcgg gcacgacaac aaccgcgact tctacatgtc gacgcaggcc gagacgatca
      541 acatgaaccg cgtcctgtac cgggagtggt ttccgcagat cgtctacgac caccatcagg
      601 cgggcccggc cggaacggtg atgttcgcgc cgccgttccg cggtcccttc aactacgtgc
      661 tcgacccgct cgtccccgtc acgaccgatt tcatcggcgc cgccatgcac gcgcggttcg
      721 ccgccgaggg gaagcccggc gtcacgatgc ggggcggggg caactattcg atgtggtgga
      781 acggcggcct gcggaccacg gcgtacttcc acaaccagat cgggctgctc accgagacga
      841 ttggaaaccc cacgcccatc gagatcccgt tcgtgcccga gcggcaggtg ccgagcgccg
      901 atctgccgta tccggtcgcg ccgcagcgct ggcgcttccg gcagtcgatc gaatactcgc
      961 tgacggccaa ccgcgccgtg atcgacacgg cctcgcgaca tcgcgagatg ctgctcctcc
     1021 gcgcgtacca gatgggacgc aactcgatcg agcgtgggaa ccgggattcg tggaccgtca
     1081 cgccgaaacg cctggcgctc gcgggcgggt cgcgcgatcg gctccgggac ccggccctcc
     1141 gggacccgcg agggtacatc cttccgtcgg accagccgga tttcctgacg gccgccaagt
     1201 tcgtttccgc gctgctcgag tccggggttg ccgtccaccg cgcgacgtcg ccgttctcca
     1261 ccggaggcag ccgcgttccg cagggatcgt tcgtcgtgaa aagcgcgcag ccgttccgcc
     1321 ctcacgtgct cgacatgttc gagccgcagg atcatcccga cgacgttccg tatccaggcg
     1381 gaccgccgac gccgccgtac gacaacgccg ggtggaccct cgcgtttcag atgggcgtca
     1441 agttcgtcag ggtgctcgac gcattcgacg ggccgctcga aaagattgac gccgcgccgg
     1501 cttccccgga gcgcctcgct cccccgggac gcgtcatcgg ggcggccggg gtcgcggggt
     1561 tcctcgcgag ccaccatcag aacgacgcct tcgtggccgt caatcgactg ctcgccgccg
     1621 gagaagaggt ctactggccc cgggatcgca cggcaggagg actgccggga ggaaccgggg
     1681 tcatgtacat tccggcccga tccgccacgc gcgcggtcct cgagagactg gccgcggatc
     1741 ggggcttgac ctttacgggc gtcgcaacgg cgcccgcggg agaggcgctg aagcttcgtc
     1801 cggttcgtat cggtttgtgg gaccgctacg gcgggtccag tccgagcggg tggacgcggt
     1861 ggctgctgga gcgctacggc ttttccttcg acgtcgtgta cgcgcagtcg ctcgacgcgg
     1921 gcgatctggc ctcgcgcttc gacgtcatcg tgctgaccga tgacgcggtg atggacactc
     1981 gccgcgggag cggtgcgccc ccctcggatc ggcttccgcc cgagttcaaa gggacgacgg
     2041 gtgagctgac gaccgcccgc accttgccgc agttgaagcg attcgtcgag gaaggcggta
     2101 ctctcgtggc gatcggcgga gcggcggcaa tcggccaggc gctgggactg ccggtgtcga
     2161 gcgcgctcgt ggacgacgcc gcgcggccgc tcgcgcgcga acggttctac gtcccgggat
     2221 ccgtgctgcg ggtgagcgtg gacaacacga cgccgctcgg atacggcttc gagcgcgagg
     2281 ttgacgtggt cttcgacaac agcccggcct tccgtcttgg cgccgacgcg ccggcgcgcg
     2341 gtctccgccg cgtcgcgtgg ttttcgagcg cggcgccgct gcggagcgga tgggctctgg
     2401 gacagcgata tctcgagaac ggcgtggccg tgatcgacgc gccgctcggg cgcggacgtg
     2461 cactgctctt cgggcccgag atcaccttcc gcgcgcagtc tcacgggacc ttcaagtttc
     2521 tcttcaacgc gatctattac gggaaggctc tgcctgtttc gagcgtggga ggctctgctc
     2581 gcgctcagta ggcctcaggg ctcagggccc aggcctcagg gctgtctcag ggaacaggga
     2641 tcagggaagg tctcttgcgc gagcgatcga cagacgtgct cgtcatcggc gccggcccgg
     2701 ccggagccgc caccgcactg agcctcgcgg ccagaggcgt cgagacggtc ctcgtcgatc
     2761 gatccgagtt cccgcgcgac aaggtgtgcg gagacgcgct gattcctgac gcgctggcgg
     2821 cgctgcgcac gctcgggctc gaacaggccg tgtgcaggcg ggcggcagtg atggaggaga
     2881 tccgcgtcta tgctccaagc ggatgcttcg tgtcgttctc ggggcgttgc gcctgcctgc
     2941 cgcggcgcgg actggaccac ctgatcctcc aggccgcagt acaggccgga gcgtcgctgc
     3001 tcacgcccta ctccgccgcg ccgatggggc gcgatcgggt gagtggagcg gttctccggc
     3061 atcgtgactc gggcgacgag gtcagactgt ctgcgcgtct gacggtcctg gcgaccggcg
     3121 cgggaatcga cggcatcacg gcattcgacg tcgtcgagcg acggcagccg agcgcggccg
     3181 cggcgcgggc gtacttcaag gttccggctg gcctcgcaca gtcgatgaag catctcgttt
     3241 tctcgtacga cgcgtcgacg gcaccaggtt atggatggat ttttccgggc ccggacgacg
     3301 tgttcaacgt cggcgtcgtg acctt
//
LOCUS       DBNGSH010000238         2086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1037381, whole genome shotgun sequence.
ACCESSION   DBNGSH010000238 DBNGSH010000000
VERSION     DBNGSH010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1037381"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(530..1852)
                     /locus_tag="AC16CY_05485"
     CDS             complement(530..1852)
                     /locus_tag="AC16CY_05485"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3353267.1"
                     /translation="MSFITKAHLSRRTFLHGVGVTLSLPLLESMIPAATALAQTAARP
                     RTRLGCIYFPHGAIMPKWTPATEGSGFELSEILQPLKPFHGQITVVSDLRHANAYGSG
                     ATANHNRSAAAYLSGAFAETGAQPSLGITVDQIAAQKIGQDTPLPSIELTIEAPSLNC
                     GDGLSCSYRDTISWQGPHSPLPMQNNPQVVFERLFGDGNTAEQRRTRREQSLSLLDSV
                     VGDVASLQRRLPATDRTRLDQYLSDVREIERRVEKAGQQLSDDLPVPPAPTGVPTDVE
                     EHIKLMYDLQVLAWQAEITRISTFLMCKELSGSVYPKSGVRDAFHTLSHHSNVQDNIN
                     RFAVLNAYHVGLFAYFLGKLRATPDGDGTLLDHSLVLYGSGMSDGNQHNHTDLPAVLA
                     GGASGRLKGGRHLRHPKNTPMANLLLAMLDRLEVPTEKFGDSNGKVSL"
     gene            complement(1883..>2086)
                     /locus_tag="AC16CY_05490"
     CDS             complement(1883..>2086)
                     /locus_tag="AC16CY_05490"
                     /inference="COORDINATES: protein motif:HMM:NF019253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HIR3353268.1"
                     /translation="AVLSRSDAFMTTATEKLYTYALGRPVHHDDMPTIRAIVRRAAAN
                     GNRFSSIVTGIVDSDAFQKRIKK"
BASE COUNT          328 a          663 c          709 g          386 t
ORIGIN      
        1 caaccgcagg acgggatttg agggcgaact ggttgacccc gccagagttg tgtcggcctc
       61 ggtcacggcg ggtgtgactc tcatcctgat cacgagactc gatttcgccg gccaccggcg
      121 tctgggccgg cgccgtgtcg agaggcgtga ctgtcggcgt cgtgatacgc gagaggggta
      181 aaccgggccc gaaacgtgac tgccgcgcag cgcatggagc gcgcgcgtcg ctggaatgga
      241 tgtggagggc caggttttcc acggcgtccg accggagcgc cgggttgaca tggcgccgcc
      301 ccagacgcgg gtcgcaggcg aaatcgtgct cattgctcac tcggtcctgg ccgaagagag
      361 cggaccgccg agaacgctct ggtgggtcgc gcgctcgatc tggcactagc ccccgagttc
      421 acttgtggca tccgcagggc cggtcttaga ccggccctgg cgggtctgcc ttcgacttcg
      481 ctcaggctag gaaagacccg cctctacgtc aacacaagtg aacctcgggc taaagactga
      541 cttttccgtt gctgtcgccg aatttctctg tcgggacttc cagcctgtcg agcatcgcca
      601 ggagcaggtt cgccatcggc gtgttcttgg gatgccgcag gtggcgccca cccttgagcc
      661 gcccagatgc gccgccggcc aggaccgccg gcagatcggt gtgattgtgc tggttcccgt
      721 cgctcatgcc gctgccgtag aggacgagcg agtggtcgag cagcgtgccg tcgccatccg
      781 gcgtcgcgcg gagcttgccg aggaagtagg cgaacagccc gacgtggtaa gcgttgagca
      841 ccgcgaaccg gttgatgttg tcctgcacgt tggaatggtg cgagagcgtg tggaacgcgt
      901 cgcggacgcc gctcttcggg tagaccgagc cgctgagctc cttgcacatg aggaacgtcg
      961 agatgcgggt gatctccgcc tgccacgcca gaacctgaag gtcgtacatc agcttgatgt
     1021 gctcttcgac gtcggtcggc acgccggtcg gcgcgggcgg caccggaagg tcgtccgaga
     1081 gctgctgtcc ggccttctcc acgcgccgtt cgatctcgcg cacgtcgctc aggtactggt
     1141 cgaggcgcgt gcgatcggta gccggcagcc tgcgctgaag cgaggcgacg tctcccacga
     1201 ccgaatcgag caggctgagc gactgctcgc gccgcgtccg gcgctgctcg gccgtgttcc
     1261 cgtcgccgaa cagccgctcg aagacgacct gcgggttgtt ctgcatcgga agcggcgagt
     1321 gcggaccctg ccaggaaatg gtgtcgcggt aagagcagct caggccgtcg ccgcagttca
     1381 ggctcggtgc ctcgatcgtc aactcgatgg acggcagcgg cgtgtcctgt ccgatcttct
     1441 gcgcggcgat ctggtccacc gtgatgccga gcgagggctg tgctcccgtt tcggcgaatg
     1501 cgccgctcaa gtacgcggca gccgaccggt tgtgattcgc agtggcgccg cttccgtacg
     1561 cgttcgcgtg ccggaggtcg ctcacgacgg tgatctggcc gtggaacggc ttcagcggct
     1621 gaagaatctc ggagagctcg aagccgctgc cctcggttgc cggggtccac ttcggcatga
     1681 tcgcgccgtg aggaaagtag atgcacccca gccgcgttcg gggacgggct gccgtctgcg
     1741 cgagcgccgt cgccgccgga atcatcgatt ccagcaaggg cagcgagagc gtcacaccca
     1801 ctccgtgcaa gaacgtccgc cgcgaaagat gcgccttggt gatgaatgac attgctgctc
     1861 cctgccccct tgcccctgcc ccctacttct tgattctctt ctgaaacgcg tcgctgtcga
     1921 cgatgccggt gacaatcgac gagaaccggt tgccgttggc cgctgccctg cggacgatgg
     1981 cgcgaatcgt gggcatgtcg tcgtggtgca cgggccgtcc gagcgcatac gtgtacagct
     2041 tctcggttgc cgtcgtcatg aacgcatccg accggctcaa gaccgc
//
LOCUS       DBNGSH010000239         2270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2226081, whole genome shotgun sequence.
ACCESSION   DBNGSH010000239 DBNGSH010000000
VERSION     DBNGSH010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2226081"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="AC16CY_05495"
     CDS             <1..612
                     /locus_tag="AC16CY_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR3353269.1"
                     /translation="LAIPLAIYGAGYAIAWSWGFAHWSPGGGKWTTGPQIAANLAINL
                     TFLGIFGTFTAMGEEIGWRGYLQPRLDAAGVRASVVVVMLCQLAYHAPLMAFAGYAEA
                     GSLATSLALFAIGDLAVTFIWARESYRARSLWPAVFLHSFHNTISQWLFPKFFTVGED
                     QSWLRGEGGLLPMAGYVLVGLAVFLWMQWRGPSWRTLVRQALP"
     gene            672..1199
                     /locus_tag="AC16CY_05500"
     CDS             672..1199
                     /locus_tag="AC16CY_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3353270.1"
                     /translation="MTDAEFQAAFDQHKGAVYRFAWRMCGSAAAAEDITQDAFVGLLR
                     HPDRFDPARGTLRTFLFVMARNLALKHLRKEHRLEPLDDEAVVAEPLDLDRGTIGDMV
                     GRAVGALLPLQREVVILVEYEGLTLAEVARAVDADVGTVKSRLHRARENLRRMLAPLE
                     RRQPSTMRTVWTTKN"
     gene            1181..1744
                     /locus_tag="AC16CY_05505"
     CDS             1181..1744
                     /locus_tag="AC16CY_05505"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3353271.1"
                     /translation="MDDQELNRLLREWKAPDVPPHLGPPHAHRSWWRWLITGTIRVPV
                     PVALAAVVLASVWTATVLTESMSTTEPAAPRRSGELARYALTGPLEGFDAVLVEVNFA
                     PGAASQAHRHPGPILGYVVDGQMRTAINHEPDQIIPAGGTFFEPHGALHTSFSSANPD
                     APVRAVAFLVVPSGSPLTERATTRTEP"
     gene            1741..>2270
                     /locus_tag="AC16CY_05510"
     CDS             1741..>2270
                     /locus_tag="AC16CY_05510"
                     /inference="COORDINATES: protein motif:HMM:NF022818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HIR3353272.1"
                     /translation="MKHLAAIAALALAQTPQGTRMPYTAVHQPEFVAAVEATFLQDDD
                     ILLGVASGKVAKAFPAADLAQHGAVSDQLPDGPISVTWCGVCNTGIVFRAEVKGRTLH
                     FQYDRMVAANEVQKDAETGSSWQQATGEAIDGPLKGTRLTLYPSVRTTWAAWRKRYPD
                     TMVLKPLPGYAERMPD"
BASE COUNT          378 a          751 c          764 g          377 t
ORIGIN      
        1 ctcgcgatac cgctggcgat ctatggcgcc ggctatgcga tcgcgtggag ctgggggttc
       61 gcgcactgga gtccgggcgg cggcaagtgg acgaccgggc cgcagatcgc ggccaacctc
      121 gcgatcaacc tcaccttcct cggaatcttc ggaacgttca cggcgatggg agaggagatc
      181 ggctggcgcg ggtacctgca gccgcgcctc gatgccgccg gcgtccgcgc gagcgtggtc
      241 gtcgtcatgc tctgccaact tgcgtatcac gcaccgctca tggcctttgc cgggtacgca
      301 gaggccggca gcctcgccac gagcctggct ctcttcgcga tcggggattt ggcggtgacg
      361 ttcatctggg cgagggagtc gtaccgcgcg cgcagcctct ggccggcagt cttcctgcac
      421 agctttcaca acaccatctc ccaatggctg ttcccgaagt tcttcacggt gggcgaggac
      481 cagagctggc ttcgaggaga gggaggcctt ctcccgatgg cgggctacgt cttggtcgga
      541 ctggccgtgt tcctctggat gcagtggcgc ggaccctcct ggcggacgct cgtgcggcag
      601 gcgctgccct gatttgcaag ggggtcgccc gtatcctcgg ggcggtctcg cggtaattgt
      661 cagcgagcca cgtgacagac gctgagttcc aggccgcatt cgaccagcac aagggcgccg
      721 tgtaccgttt cgcctggcgc atgtgcggct cggcggccgc ggcagaggac atcacacagg
      781 acgcgttcgt cggcctgctg cgacatccag atcgattcga tccagcgcgc gggacgctgc
      841 gcacgttcct gttcgtgatg gcgcggaacc tcgccctcaa gcacttgcgc aaggaacatc
      901 ggctcgagcc gctcgatgac gaggcggttg tcgcagagcc gctcgacctc gatcgcggca
      961 ccatcggcga catggtcggc cgggccgtgg gcgcgctgct gccgctgcaa cgcgaggtgg
     1021 tgatcctcgt cgagtacgaa ggcctgacgc ttgccgaggt cgcgcgcgcc gtagacgcgg
     1081 acgtcggcac ggtcaagtcg cggctgcatc gcgcccgcga aaatctgcgc cgcatgctgg
     1141 cgccgctcga gaggcgtcaa cccagcacca tgaggaccgt atggacgacc aagaactgaa
     1201 tcgactgctg cgtgaatgga aggcaccgga tgtaccgccg catctcggtc ctcctcacgc
     1261 gcatcggtcg tggtggcgct ggctgattac cggcacgatt cgcgtgcccg tgccggtcgc
     1321 gctcgcggcg gtcgtgctgg catcagtctg gaccgccacg gtgctgaccg aatcgatgtc
     1381 aacgacggaa ccggccgccc ctcggcgctc tggcgagctg gcgagatacg cgctgacggg
     1441 tcctctcgaa gggttcgacg cagtgctcgt ggaagtgaac ttcgcgccag gcgctgcgag
     1501 ccaggcgcat cgtcatcccg gtcccatcct cgggtacgtc gtcgatgggc agatgcgaac
     1561 ggccatcaat cacgagcccg accagatcat ccccgcgggc ggcacgttct tcgaaccgca
     1621 tggcgccctt cacacgtcgt tcagttccgc gaaccccgac gcgcccgtcc gagccgtggc
     1681 attcctggtg gtacccagcg gcagcccgtt gacggagcgc gcgacgacaa ggacagaacc
     1741 atgaaacacc tggccgcaat cgcagcgctg gccctcgcgc agaccccgca ggggacgcgg
     1801 atgccctaca cggctgttca ccagccggaa ttcgtggctg cggtagaagc gacgtttctt
     1861 caggacgacg acatcctgct cggcgtcgcg agcggcaagg tggccaaggc gttcccggcg
     1921 gcagacctcg ctcagcatgg cgcggtgtcc gaccagctcc cggacgggcc aatctcggtg
     1981 acatggtgcg gagtctgcaa cacaggcatc gtctttcgcg ccgaggtgaa ggggcggacg
     2041 ctgcacttcc aatacgaccg aatggttgcc gcgaacgagg tgcagaagga cgcggaaacc
     2101 ggcagcagct ggcagcaggc cactggcgaa gcgatcgacg gccccctcaa aggcacgcgc
     2161 ctgacgctct atccgtcggt tcgcaccaca tgggcagcgt ggcgcaagcg gtatcccgac
     2221 acgatggtgc tcaagcccct gccgggatac gcggagcgca tgcccgatca
//
LOCUS       DBNGSH010000240         3320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3244564, whole genome shotgun sequence.
ACCESSION   DBNGSH010000240 DBNGSH010000000
VERSION     DBNGSH010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3244564"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /locus_tag="AC16CY_05515"
     CDS             complement(<1..1858)
                     /locus_tag="AC16CY_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3353273.1"
                     /translation="MISRIVDWCAGNRALVFMATAALTVWGVWAMTATPLDAVPDISD
                     VQVIVSTEWAGRSPDIIEDQVTYPIVTALISAPRVRTVRGFTDFGISYVYVIFEDGTD
                     IYWARSRVVEYLQGLRGRLPEGSNPTIGPDATGVGWVFQYALVDDTGQHDLAELRGVQ
                     DWFLRYNLASVDGVAEVASIGGFVKQYQVNLDPTRLAAYDKSVKDVVQAIRASNNEVE
                     GRLLEFSGREYMVRGRGYLTSVDDIAAVSLGANARGTPVRVGDVADVRLGPDIRRGVA
                     ELDGKGEVVGGIVVMRFGENALRVIERVKARLEEIKSALPSGVRIVPTYDRSGLINES
                     IATLRRALVEEAIVVSLVILVFLFHVRSALVPILALPIAVVASFIPMYHLDVTSNIMS
                     LGGLALAIGVLVDASIVMVENAYRHVSEAGADGAAAAYAEQPRVIIGAAKQVARAIFF
                     SLAIIIVSFVPVFLLEAQEGRMFRPLAFTKTSAMVFASVLSITLVPVLMTLFIRGRRL
                     KREAVNPVSRLFAWLYEPVLRAALRFKWTALLLNFAVVPLTIPLLFVIGSEFMPPLYE
                     GSMLYMPTSPPGMSITEATRLLQVQDRLLRQVPEVEQVFGTVGRGTTATDNSP"
     gene            complement(1923..2348)
                     /locus_tag="AC16CY_05520"
     CDS             complement(1923..2348)
                     /locus_tag="AC16CY_05520"
                     /inference="COORDINATES: protein motif:HMM:NF017559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR3353274.1"
                     /translation="MTTRVRGVMVAVMAGVIAAIAGCGQPQPASQNAAEGAARGFVIT
                     LRTDPDPVRTGENTLEATVVQDGKPVTDATVSAEFFMAAMPAMNMPEMRTTADLAHEG
                     NGTYRGKGQVAMAGAWDVTVKALRGGQEIGRTKTTVMAK"
     gene            complement(2434..>3320)
                     /locus_tag="AC16CY_05525"
     CDS             complement(2434..>3320)
                     /locus_tag="AC16CY_05525"
                     /inference="COORDINATES: protein motif:HMM:NF017559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR3353275.1"
                     /translation="AVRGMHIVPGQTLYKVADLSVVWVEADVYEQEMALANVGQRAIV
                     TLDAYPRESFDGRAIYVYPFVEENTRTTKVRFQFSNASGRLKPGMYANVELQGAGGMS
                     LTVPANAVLDSGIEQVVFVARGEGRFTPRTVKAGRSFGDRVEIVGGLEEGEQVAIGAT
                     FFLDSESQLRAGLQNFEAAPVDARAQPATGEPLDIGFRSQPDPPKAGEGVFEVRVNGA
                     GGKPVTDAEVSVLFFMPAMPTMNMPAMRNDTKLPHAGGGVYRGPGQVLMAGRWDVTVT
                     VGRGGRQIGQKQFAITAR"
BASE COUNT          546 a         1179 c         1049 g          546 t
ORIGIN      
        1 tcgggctgtt gtcggtcgcc gtcgtcccgc ggccgaccgt cccgaacacc tgttcgacct
       61 ccgggacctg gcgcagcagc cggtcctgga cctgcagcag gcgcgtcgcc tcggtgatcg
      121 acatcccggg cggcgacgtc ggcatgtaga gcatcgaccc ttcgtagagc ggcggcatga
      181 actcgctgcc gatcacgaac agcagcggga tggtaagcgg gacgaccgcg aagttgagga
      241 gcaaggccgt ccacttgaag cgcagggcgg cgcgaagcac cggctcgtac aaccacgcga
      301 acaggcgcga cacggggttg acggcctccc gtttcaaccg ccgtccgcgg atgaagagcg
      361 tcatcagcac ggggacgagc gtgatcgaca acaccgacgc aaacaccatc gcggaggtct
      421 tcgtgaatgc cagcggacgg aacatgcggc cttcctgcgc ctccagcaga aagaccggga
      481 caaaggacac gatgatgatc gccagcgaga agaagatcgc acgcgccacc tgcttggccg
      541 ccccgatgat cacgcgcggt tgctctgcgt acgcggccgc cgccccgtcg gcgcccgctt
      601 ccgacacgtg gcggtacgcg ttctcgacca tcacgatcga cgcgtcgacg agcacgccga
      661 tggcgagcgc cagcccgccg agcgacatga tgttcgaggt gacgtccagg tggtacatcg
      721 ggatgaacga ggccaccacg gcaatcggca gcgcgaggat cggcaccagc gccgaacgga
      781 cgtggaacag gaacacgagg atgacgagcg agacgacgat ggcttcttcg acgagcgcgc
      841 ggcgcagggt ggcgatcgac tcgttgatca gtcccgaccg gtcgtaggtg ggaacgatgc
      901 gcacgccgga cggcagcgcg ctcttgatct cttcgagcct ggccttgacg cgctcgatga
      961 cgcgaagagc gttctcgccg aaccgcatca cgacgatgcc gcccaccacc tcgcccttgc
     1021 cgtccagttc ggcgacgccc cgccggatgt ccgggccgag gcgcacgtcc gccacgtccc
     1081 ccacgcggac gggtgtgccg cgggcgttcg ccccgagcga gacagcggcg atgtcgtcca
     1141 ccgacgtcag gtagccccgg ccgcgcacca tgtactcgcg tcccgagaac tccaggagcc
     1201 tgccttcgac ctcgttgttg ctcgcgcgaa tcgcctggac gacgtccttg accgacttgt
     1261 cgtaggccgc cagcctggtc gggtccaggt tgacctggta ctgcttgacg aagccgccga
     1321 tgctggccac ttccgcgacg ccgtcgaccg aggcgaggtt gtagcggaga aaccagtcct
     1381 gcaccccgcg cagctccgcg aggtcgtgct gtccggtgtc gtccacgagg gcgtactgaa
     1441 agacccagcc gacgcctgtc gcgtcggggc cgatcgtcgg attgctcccc tcgggcaggc
     1501 ggcctcgaag tccctgcagg tactcgacga cccggctgcg cgcccagtag atgtcggttc
     1561 cgtcctcgaa gatgacgtag acgtacgaga tgccgaagtc ggtaaacccg cgaaccgtgc
     1621 gcacgcgcgg cgccgaaatc agcgcggtga cgatcggata cgtgacctga tcctcgatga
     1681 tgtccggact gcgccccgcc cactcggtgg acacgatcac ctggacgtcg gagatgtccg
     1741 gtaccgcatc caggggcgtc gccgtcatcg cccagacgcc ccacaccgtc agcgcggccg
     1801 tggccatgaa caccagggcc cggttgccgg cgcaccagtc aacgattctc gagatcatgt
     1861 gtctccggtc cggctgcctt cgacttcgct caggccaagg aagccggaca ctacgtacgt
     1921 gctcacttcg ccatcacggt cgtcttcgtg cgcccgatct cctgtccgcc tcgcagggcc
     1981 ttcacggtca catcccacgc gccggccatc gcgacctgcc ccttgccgcg gtaggtcccg
     2041 ttgccttcgt gcgcgaggtc ggcggtcgtc cgcatttccg gcatgttcat cgccggcatc
     2101 gcagccatga agaactcggc cgacaccgtc gcgtcggtga ccggcttgcc gtcctgcacg
     2161 acggtggctt cgagcgtgtt ctcgcccgtg cgcacgggat ccggatcggt gcgcagagta
     2221 atgacgaacc ctcgagccgc accctccgcc gcgttctggc tcgcgggctg cggctggcca
     2281 cagccggcga tcgcggcgat cacgcccgcc atcaccgcca ccatcacccc tcttacgcgc
     2341 gtcgtcatcg ttcctccttg gtttccggtc tttaccttcg tacacttcgt gcccttcgtg
     2401 gccaaccctt cgtgcccttc gtggttagcc ccgctatctg gccgtaatag cgaactgctt
     2461 ctgtcctatc tgcctgccgc ctcgtcccac ggtcacggtc acatcccatc gtccggccat
     2521 gagcacctgc ccgggaccac ggtacacgcc gccgccggcg tgcggcagct tcgtgtcgtt
     2581 tcgcatggcg ggcatgttca tcgtcggcat ggccggcatg aagaacaaca ccgagacctc
     2641 ggcgtcggtc acgggcttgc caccggcccc gttcacgcga acctcgaaaa cgccctcgcc
     2701 ggccttcggc ggatcgggct gcgagcggaa gccgatgtcg agcggctccc ctgtcgccgg
     2761 ctgcgcgcgg gcgtccaccg gggccgcctc gaagttctga aggcccgcac gaagctggct
     2821 ctccgaatcg aggaagaagg tggcgccgat ggccacctgc tcgccctcct cgagccctcc
     2881 cacaatctca acgcgatcgc cgaaggaccg gccggccttc acggtccgcg gggtgaagcg
     2941 cccttcgccg cgcgccacga agacgacctg ctcgatgccc gagtcgagga cggcgttggc
     3001 cggtaccgtc aggctcatgc cgcccgcgcc ttgcagctcg acgttcgcgt acattcccgg
     3061 cttcaggcgc ccggaggcgt tcgagaactg gaaccgcacc ttcgttgtgc gcgtgttctc
     3121 ttcgacgaac ggatagacat agatggcgcg gccgtcgaag gactctcggg ggtaggcgtc
     3181 gagcgtgacg atggcgcgct gcccgacgtt cgccaacgcc atctcctgct cgtacacgtc
     3241 ggcttcgacc cacacgaccg agagatcggc aactttgtac agcgtctgcc cgggcacgat
     3301 gtgcatgccg cgcacggcct
//
LOCUS       DBNGSH010000241         4195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1206937, whole genome shotgun sequence.
ACCESSION   DBNGSH010000241 DBNGSH010000000
VERSION     DBNGSH010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1206937"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="AC16CY_05530"
     CDS             complement(<1..470)
                     /locus_tag="AC16CY_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353276.1"
                     /translation="MKLLVALIALATCLCADLRAQEVERTPRAPGPIAESTDRIARET
                     WPTEARSFTIDEDGRPRFRSGVTATLPPPLWQLTPGDTSPVPIRGALSHREMVAAVTP
                     EPFRDNVMYPGVSVDPGAIYHGIVKAWRDWQARRIHERVTKELEEFLRATAERES"
     gene            complement(528..2420)
                     /locus_tag="AC16CY_05535"
     CDS             complement(528..2420)
                     /locus_tag="AC16CY_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3353277.1"
                     /translation="MRHRPFRPLLVLVLTLAAAPASAQATAYRAPRTDGGQPDLQGVW
                     NFDSGVPLERPPAFASKTFFTKEEFEKQRATMRNGLAAIAKLAPVEAVGFDWIDDTLH
                     VNDLRTSLITYPDNGRLPTILEGVRRMPRFDELISVLGGGGSNVTPASFQAFAAMFAA
                     GARRDSYTDFMMSERCLIAAEVPFVPQITDSYVQIVQSRDSIVLLTEFDRRIVALDGP
                     STPLGAGPSTPLGAGRPVIGARRSWTGTSRGRWEGETLVVETKGFNDRLPSFAGAGTG
                     RDKVVTERFTRTAIDVIEYSATVVDPATFRDRIELSFPMARVDARLYEGACHEGNRSL
                     AHALSAARKEDETRPRQQLTVLDRQGEVVGRVGEAGFYSQAAFSPDGSRVAVVRDGAS
                     QDIWVIDAATGRGTAITSDAFQDRAPVWSPDGSRIAYVSVRDNMPAIYQRASNGQGGE
                     ELLYRHASSGLIVLTDWSPDGRVLCFWSENAMFLLPVSGERTPVALGDGEFFGRGGRF
                     SPNGRLLAFNSNQSGRFQIYVKELGTATNPGASPASARVQVSVDGGVGGIFWRRDGKE
                     LFYLSQPNQALMAVDVESDTPLRVGAPRVVFKLPSTIGAPAQLSSISTPDGQRFVFAM
                     SVAAVK"
     gene            complement(2546..2848)
                     /gene="rpmE"
                     /locus_tag="AC16CY_05540"
     CDS             complement(2546..2848)
                     /gene="rpmE"
                     /locus_tag="AC16CY_05540"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR3353278.1"
                     /translation="MKEGIHPKYQEVEARCACGNTFKTRSTKPELHLEICSACHPFFT
                     GRQKLIDTEGRVERFTKKFGAQTSDQRKTAARAAKTTKTAKAAASKKAKPAKTATA"
     gene            complement(2912..3610)
                     /locus_tag="AC16CY_05545"
     CDS             complement(2912..3610)
                     /locus_tag="AC16CY_05545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3353279.1"
                     /translation="MRTVMESPLLSVVMPVFNERATIDEIIPRVLAIPMRVELIVVDD
                     GSTDGTRERLEELRQRHGFALLLQPKNLGKGAALKRAFAAATGDIVAVQDADLEYSPE
                     ELPHLAELIIEGRADVVYGSRFLGRHRVFLFTHYLGNRILTLATNVLYNTMLTDMETC
                     YKVMRREVLEGLDLRSRGFGIEPELTAKIFKRGYRVYEVPITYDGRGYDEGKKITWKD
                     GLTALWVLIRHRLG"
     gene            complement(3662..>4195)
                     /gene="rho"
                     /locus_tag="AC16CY_05550"
     CDS             complement(3662..>4195)
                     /gene="rho"
                     /locus_tag="AC16CY_05550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR3353280.1"
                     /translation="MVIEKAKRLVEHKKDVVILLDSITRLARAYNTIVPASGKVLSGG
                     VDSNALQRPKRFFGAARNIEEGGSLTIVATALVDTGSRMDEVIFEEFKGTGNSEIHLD
                     RKLTDHRVFPSIDISKSGTRKEELLLPKEELNRVWVLRKVLTPLSPSEAMELLLSRMS
                     KTKTNAEFLASMAAGKT"
BASE COUNT          662 a         1358 c         1417 g          758 t
ORIGIN      
        1 gattcccgct cggcggttgc gcgaagaaat tcctcgagct ctttcgtgac gcgctcgtgg
       61 atgcggcgcg cctgccagtc gcgccacgcc ttcacgatgc cgtggtagat cgcgccggga
      121 tcgacgctca cgccgggata catgacgttg tcccggaacg gttccggcgt gacggcggcg
      181 accatctctc gatgggagag cgcgccgcga atcggcaccg gtgacgtatc accgggagtg
      241 agctgccaca gcgggggagg cagcgtcgcc gtcacgcccg atcggaagcg cggccgcccg
      301 tcctcgtcga tcgtgaacga ccgggcctcg gtcggccacg tttcacgtgc gattctgtcg
      361 gttgactcgg caatcggccc gggcgccctc ggggtcctct ccacctcttg tgcccggaga
      421 tcggcgcaca ggcacgtcgc cagagcgatg agggcgacga gcagcttcat gaagggttag
      481 acggccggcg gcgggagacg gccgccggcg cctctacctc gtgtggacta ttttaccgcc
      541 gcgacactca tcgcaaatac gaagcgctgt ccgtccgggg tgctgatgct gctgagttgc
      601 gcgggagcgc cgatggtgct gggtaacttg aagaccaccc gcggcgcgcc gacccgaagt
      661 ggagtgtcgc tttcgacatc aaccgccatg agtgcctggt tgggctggga gagatagaac
      721 aactccttgc cgtcgcgacg ccagaagatg ccgccgacgc cgccgtcgac agagacctgc
      781 acgcgcgccg acgccggtga cgcacccggg ttggtcgccg tgccgagctc cttcacatag
      841 atctgaaacc ggcccgactg gttcgagttg aacgcgagca gccggccgtt tggcgagaac
      901 cggccgccgc gtccgaagaa ctcgccgtcg cccagcgcaa ccggcgtacg ctcgccggag
      961 accggtagca ggaacatcgc gttctcggac cagaagcaga ggacacggcc gtcaggcgac
     1021 cagtccgtca ggacgatcaa cccgctgctc gcgtgccgat agagcagttc ctcgccgcct
     1081 tgtccattcg acgcccgctg gtagatcgcc ggcatgttgt cgcgcacgga cacgtaggcg
     1141 atccggctcc cgtcgggcga ccagaccgga gcgcgatcct ggaacgcgtc ggacgtgatc
     1201 gcggtccctc ggccggtggc cgcgtcgatg acccagatgt cctggctggc gccgtcgcgg
     1261 acgaccgcca cgcgggaccc gtccggagag aacgcggcct gtgaatagaa accggcctct
     1321 cccacccggc cgacgacctc gccctgcctg tccagcaccg tgagctgctg ccgcggcctc
     1381 gtctcgtctt ccttgcgggc tgccgacagc gcatgtgcca ggctgcggtt gccttcatga
     1441 catgcgcctt cgtagaggcg cgcgtcgacc cgggccatcg ggaacgacag ctcgatacgg
     1501 tcccggaagg tcgcgggatc cacgaccgtg gcggagtatt cgatcacgtc gatcgcggtc
     1561 ctcgtgaagc gctcggtcac caccttgtcg cgtccggtgc ccgccccggc gaagcttggc
     1621 aaacgatcgt tgaagccttt cgtctcgacc accagcgtct cgccttccca gcggcctctg
     1681 gacgtgcccg tccagctgcg ccgagcgccg atcaccggcc tgcctgcccc gagcggagtc
     1741 gaggggcctg ccccgagcgg agtcgagggg ccgtcgaggg cgacgatgcg gcgatcgaac
     1801 tccgtgagga gcacgatgga atcccgggat tgaacgatct ggacgtagct gtcggtgatc
     1861 tgcggcacga acggcacttc cgctgcgatc agacatcgct cggacatcat gaaatccgtg
     1921 taactgtccc ttcgcgcgcc cgcggcgaac atcgccgcga acgcctggaa cgatgccggc
     1981 gtcacattgc tgccgcctcc gccgagcacc gagatcagct cgtcgaagcg aggcatgcgc
     2041 cggacgccct cgaggatggt gggcagccgg ccgttgtccg ggtacgtgat cagcgacgtg
     2101 cgcaggtcgt tgacgtggag cgtgtcgtcg atccagtcga agccgacggc ttccactgga
     2161 gcgagcttgg cgatcgcagc cagcccgttc cgcatcgtgg cgcgctgctt ttcgaattcc
     2221 tctttcgtga agaacgtctt gctggcgaac gcgggcggcc gttcgagcgg cactcccgag
     2281 tcgaagttcc acacaccctg aaggtccggc tggccgccgt cggttcgcgg cgcccggtat
     2341 gccgtggcct gtgccgaggc gggcgcagcc gcaagcgtca ggacgagaac gagcagcggg
     2401 cggaaggggc gatgacgcat ggccggctcc tacgattgtc gataatctag gtatcgctcc
     2461 cgctattgtc gatagtcgag gtatgcgtgt caagcgacac acagaagggg gaagggggaa
     2521 gggggaacgg gggcggagcg ggagctcagg cggtcgccgt cttggcgggc ttggccttct
     2581 tcgatgccgc ggctttggcg gtcttcgtcg tcttggcggc gcgggcggcc gtcttccgct
     2641 gatccgaggt ctgcgcgccg aacttcttgg tgaaccgctc gacgcggcct tcggtgtcga
     2701 tcagtttctg acgcccggtg aagaacgggt ggcacgccga gcagatttcc aggtgcaact
     2761 cgggcttggt cgaacgggtc ttgaaggtgt tgccgcacgc acagcgagcc tcgacctcct
     2821 ggtacttggg atggatgcct tccttcacgg ccactccttc tgcatggaca aaccccgatt
     2881 atagtcgatt ctctcgtctg gaccgggcgg gtcagcccag ccggtgccgg atcaggaccc
     2941 agagcgccgt cagcccgtct ttccaggtga tcttctttcc ctcgtcgtag ccccggccgt
     3001 cgtaggtgat gggcacctcg tacacccggt agccccgctt gaagatcttg gcggtcagtt
     3061 cgggctcgat gccgaaaccg cgggagcgga gatcgaggcc ctcgagcacc tcgcgccgca
     3121 tcaccttgta gcaggtctcc atgtcggtga gcatcgtgtt gtataggacg ttggtcgcca
     3181 gcgtgaggat ccggttgccc aggtagtgcg tgaagagaaa cacccggtgg cggccgagaa
     3241 acctcgaccc gtagacgacg tcggcgcgcc cctcgatgat gagttcggcc aggtggggca
     3301 gctcttccgg cgagtattct aggtctgcat cctggaccgc cacgatgtcg ccggtcgccg
     3361 cggcgaaggc gcgtttgagc gccgcgcctt ttccgaggtt cttcggctgc agcagcagcg
     3421 cgaagccgtg ccgctgccgg agctcttcga gcctttcgcg ggtgccgtcg gtggagccgt
     3481 cgtcgacgac gatgagctcc acgcgcatgg ggatggcgag gacgcggggg atgatctcgt
     3541 cgatcgtggc ccgctcgttg aagaccggca tgaccaccga caggagcggc gactccatga
     3601 cggttctcac gccgtggcga cggtgctgac cccggtcagt cacgcgtgtc gtgtcttggc
     3661 gctacgtctt gccggccgcc atcgacgcaa ggaattcggc gttggtcttc gtcttgctca
     3721 tcctggagag cagcagttcc atggcttcgc ttggtgagag aggcgtcagc accttgcgga
     3781 gcacccagac gcggttgagc tcttccttcg gcaggagcag ctcctccttc cgcgtgccgc
     3841 tcttggagat gtcgatcgag gggaacacgc ggtggtcggt cagcttgcga tcgaggtgga
     3901 tttcggaatt gcccgtgccc ttgaactcct cgaagatcac ctcgtccatc cgcgacccgg
     3961 tgtcgacgag cgcggttgcc acgatcgtca gcgagccgcc ttcttcgatg ttgcgggcgg
     4021 ccccgaagaa ccgcttcggg cgctgcagcg cgttgctgtc gacgccgccc gacagcacct
     4081 tgccggacgc cggcacgatg gtgttgtagg cgcgcgccag ccgcgtgatg gagtcgagga
     4141 ggatgacgac gtccttcttg tgctcgacga gacgcttggc cttctcgatc accat
//
LOCUS       DBNGSH010000242         2626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4433610, whole genome shotgun sequence.
ACCESSION   DBNGSH010000242 DBNGSH010000000
VERSION     DBNGSH010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4433610"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="AC16CY_05555"
     CDS             <1..293
                     /locus_tag="AC16CY_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353281.1"
                     /translation="LPAVADAGQVYGTIVMGGQGVKDASVEIKCGDAAAATGSTAADG
                     AYRINVPQQGQCTLALPAYAGASIVVFSSPNPALYNLELVRLADGKHELRRR"
     gene            296..1309
                     /locus_tag="AC16CY_05560"
     CDS             296..1309
                     /locus_tag="AC16CY_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353282.1"
                     /translation="MSDGSVPVPTPAPAPAPADNPGRGPFIELVKVLALPVVTLVLGF
                     LFNSSLSERQQIDNNVRLYAEMMGRREEADSNLRKDMFNSILTTFLAKDPNQRLSSED
                     QIRQQILSLELLAYNFHESLDIAPLFKDVARRIAEEKGAATGELRSRLESVALQVIEH
                     QFTALSDVGMVERGDALPARINDFQAHIMFGANTVPDPSVKPGEGAARLCLSMEATDG
                     TRHYRQFRLELIGHNESAHEVQVRLFVSQILTEAECQSATLDLQSKKEVDTNFLVGLF
                     DFPMIDNTRLSGGERCAVSLTALNPNVVSIALAYFPASRASLKDKPYYDEVIRILRNR
                     SQR"
     gene            complement(1343..2215)
                     /locus_tag="AC16CY_05565"
     CDS             complement(1343..2215)
                     /locus_tag="AC16CY_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3353283.1"
                     /translation="MRRLRLTIVLVALTITSALAQSPPGPTFDVVSIKRNTSNSLGNS
                     MRQAPDGGFTMVNSSVQFLIASAYPTSGASEVVGLPAWATSERYDVMATAALPASPSA
                     DDRREMMRAMLADRFKLKAHYEKREQPAFALVVARGDGKLGPQVKPAEVDCEARFAAQ
                     RAEAEAARGGTPPERSVPVLPAPRSPVPPCTTRMTGNRMEGDMTMATLARFLRPAAGR
                     YIVDQTGLTGYYRVVLEYARMAGLRGPDVASTPGEAPSVFTAVQEQLGLKLESTRTEL
                     DMLVIDSIERPSEN"
     gene            complement(2240..>2626)
                     /locus_tag="AC16CY_05570"
     CDS             complement(2240..>2626)
                     /locus_tag="AC16CY_05570"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3353284.1"
                     /translation="YMDDNDAKNTGGKFRMIPIFDKAVAMSVATKGLKVMVGSGADGS
                     TYPHGTQALDFEALVKRSGMAPARAIQAGTIVNAEVMGWQNDVGSITKGKFADLVAVA
                     GDPLADIAELQRVKFVMKGGKVIRND"
BASE COUNT          433 a          895 c          839 g          459 t
ORIGIN      
        1 tgctgccggc cgtcgccgac gcgggacagg tgtacgggac gatcgtcatg ggaggccagg
       61 gcgtcaagga cgccagcgtc gaaatcaagt gcggcgatgc cgcggccgcc acgggatcca
      121 ccgccgccga cggcgcctac cggatcaacg tcccgcagca gggccagtgc acgctcgcgc
      181 tcccggccta cgccggggcg tcgattgtcg tcttctccag cccgaatccc gccctgtaca
      241 acctggaact cgttcgcctc gcggacggca agcacgagct gcggcggcgg tagccgtgag
      301 cgacggcagc gttcccgttc cgacgcccgc ccccgccccc gcccctgccg acaacccggg
      361 ccgggggccg ttcatcgagc tcgtcaaggt gctggccctg ccggtggtga cactggtcct
      421 cggcttcctg ttcaactcct ctctgagcga gcgtcagcag atcgacaaca acgtccggct
      481 gtacgccgag atgatggggc gccgtgaaga ggccgacagc aaccttcgca aggacatgtt
      541 caattcgatc ctgaccacgt tcctcgccaa ggaccccaat cagcggctgt cgtccgagga
      601 tcagatccgc cagcagatac tcagcctcga gctgctcgcg tacaacttcc acgagtcgct
      661 cgacatcgca ccgctcttca aggacgtggc gcggcggatt gccgaggaga agggcgccgc
      721 caccggcgag ctgcgctcgc gcctcgagtc ggtggcgctg caggtcatcg agcaccagtt
      781 cacggcgctc tccgacgtcg gcatggtgga gcgcggcgac gccctgccag ccaggatcaa
      841 tgacttccag gcgcacatca tgttcggcgc caacaccgtt ccggatccga gcgtgaagcc
      901 gggcgagggg gccgctcgcc tctgcctgtc gatggaagcg accgacggga cgcggcacta
      961 ccggcagttc aggctggagc tcatcggaca caacgagtcg gcgcacgaag tgcaggtgag
     1021 gctgtttgtg tcgcagatac tgacggaggc cgagtgtcag tcggccacgc tggatctcca
     1081 gagcaagaaa gaagtggaca cgaatttcct ggtcgggctg ttcgactttc cgatgatcga
     1141 caacacccgc ctgtccggcg gcgagcgctg tgccgtgtcg ctcacggcgc tcaatccgaa
     1201 cgtggtgagt atcgcgctgg cgtactttcc cgcctcgcgc gcgagcctga aggacaagcc
     1261 gtattacgac gaggtgattc gcattctgcg caaccggtcc cagcgatagc tcagagccaa
     1321 gcgccgagct gttccctccg agtcagtttt cggaaggcct ctcgatgctg tcgatcacga
     1381 gcatgtcgag ctcggtgcgc gtcgactcga gcttcagccc gagctgctcc tgcacggccg
     1441 tgaagaccga cggcgcttca cccggtgtgc tcgccacgtc tggtccgcgt agacctgcca
     1501 ttcgcgcata ctccagcacc acccggtagt agccagtcag gccggtctgg tcgacgatgt
     1561 atcgacccgc tgcggggcgc agaaatctgg cgagcgtcgc catcgtcatg tcgccttcca
     1621 tcctgttgcc ggtcattctc gtggtgcagg gcgggacggg tgaacgggga gctggcagca
     1681 ccggcaccga tcgctcgggc ggcgtaccgc cccgcgcggc ttccgcctcg gcgcgctgtg
     1741 ctgcaaagcg cgcctcgcaa tcgacttcgg caggctttac ctgtggaccg agcttaccgt
     1801 cgccgcgcgc cacgacgagc gcaaacgccg gctgctcgcg cttttcgtaa tgcgccttga
     1861 gcttgaagcg atcggccagc atcgcccgca tcatctcccg tcgatcgtcg gccgacggac
     1921 tcgccggcag cgcggccgtc gccatcacgt cgtagcgctc cgatgtggcc caggccggca
     1981 gtccgacgac ttccgacgct cccgaggtgg gataggccga ggcgatcagg aactggaccg
     2041 agctgttcac catcgtgaag ccgccatcgg gggcctggcg catgctgttg ccgagcgaat
     2101 tgctcgtgtt ccgcttgatc gacacgacgt cgaaggttgg tcccgggggc gactgcgcaa
     2161 gggccgacgt gatggtcagt gccacgagca cgatggtcag tcgtagacgt cgcatgcctg
     2221 cctcccagcc gtcacaccgt cagtcgttgc ggatgacctt gccgcctttc atcacgaact
     2281 tcacccgctg aagctcggcg atgtcggcga gcgggtcacc ggccacggcg acgagatccg
     2341 cgaacttccc cttggtgatc gacccgacgt cgttctgcca ccccatcact tcggcgttga
     2401 cgatcgtccc tgcctggatg gcgcgcgccg gggccatgcc cgaacgcttg acgagcgcct
     2461 cgaagtcgag cgcctgggtg ccatgcggat acgtcgatcc gtcggcgccg ctgccgacca
     2521 tgaccttgag ccccttcgtg gcgaccgaca tggcaacggc cttgtcgaag atcgggatca
     2581 tccggaactt gccgccggtg ttcttcgcgt cgttgtcgtc catgta
//
LOCUS       DBNGSH010000243         6882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_358423, whole genome shotgun sequence.
ACCESSION   DBNGSH010000243 DBNGSH010000000
VERSION     DBNGSH010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_358423"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="AC16CY_05575"
     CDS             <1..408
                     /locus_tag="AC16CY_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3353285.1"
                     /translation="EKTTPGRSTFDVRIGDTNWPCQKPPWGRLTAIDTSTGEFAWQVP
                     LGITEQLPAGKQNTGRPVLAGPIVTASGLVFIASTDDNRFRALDAKTGKELWVMRLER
                     RGNANPITYQGTDGRQYVAVVATDTLMVYALPK"
     gene            complement(480..3197)
                     /gene="polA"
                     /locus_tag="AC16CY_05580"
     CDS             complement(480..3197)
                     /gene="polA"
                     /locus_tag="AC16CY_05580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR3353286.1"
                     /translation="MARPTLFLIDGSSQMYRAYHAFRGRGLSNQEGRSTHAVYVFVTM
                     LRKLIADHKPAYLAASFDLPGPTFRDEIATDYKANRAEMPDDLVEQINWVHEACEAMG
                     VPILSFPGYEADDVIGTVASRAAAAGYQVAIVSIDKDFFQLVRDGSVRVYDPREDGAW
                     FDEQAVAEKFGVKPTQVVDVLALVGDSSDNVAGVPGIGKKGAIDLVTQFGSLDGLLAH
                     ASELKGKQREALESHRDVARRSRELVTIRTDVPLEPDFETLRYRGASKDRCYELFTRL
                     DFRTLVNEYAPDASSVQADYGVVATEPELDALAAHLRSTGVMAIHVIADHASAVQAGV
                     VGIAVSAADRWARYVPVGHAGDTAGGSLLASAEAPTQLDRGLVFDRLGPLLEDETLAK
                     RGHDLKGSSLILSRQGITLRGLAFDSMLASYLLDATRPSHDLEDAALQHLGYRARTAD
                     DVRGRGARAVAFSQVSPENLLGFAGERADLAWQLSGKLAPQLVSEQLEPVFRDLEMPL
                     IPVLAHVEQAGVLIDTPLLAAQSRHFEQELASFTARIYELAGEEFNINSPQQLGRILF
                     EKLALPVARKTGKTRSASTAAEVLEELATGHELPRLVIDWRALQKLKSTYIDALPLLV
                     NPATGRVHTSFNQAVAATGRLSSSDPNLQNIPIRTEHGRDIRRAFIAAPGHVLISADY
                     SQIELRVLAHLAGEDALIEAFRAGEDIHDRTAAKLFGTDSGLSRHELRSRSKMVNYAV
                     LYGKTQFTLAKDIGVTQEAAQAFIDAYFAGYPRVREFIDHTLEEARRTGVVKTMFGRR
                     RLTPNLTSRNFQVRSQAERETVNMPIQGTAADILKKAMIDLHAALPRQGFRARMILTV
                     HDELLFECPKEEAESAAALVRERMESAASLAVPLTVDVGIGENWRDAKA"
     gene            3221..3985
                     /locus_tag="AC16CY_05585"
     CDS             3221..3985
                     /locus_tag="AC16CY_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353287.1"
                     /translation="MVPARFRVALSAAACATVAWSCGSGLLGTQYEYEEQIRLSVDGS
                     ARVDVSSSVPALVALRGVDLPLDPAAEVDRSKIRSLFEGPGATVASVRRSRRAGRQFV
                     HVRVEVDDIRGLSRIAPFSWSTYQLNRRGDVLEYAQTVGASSGKGSSEAAWTGNEVVA
                     FRMHIPSVIVFHNAPSRKVRRGNIVEWEQPLAERLKNTPVDIRVNMEAESILYTTLLL
                     FGSTIVLAAIAFGVVIWRVARHGRRNPPKPAIPAAS"
     gene            complement(3994..4536)
                     /locus_tag="AC16CY_05590"
     CDS             complement(3994..4536)
                     /locus_tag="AC16CY_05590"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3353288.1"
                     /translation="MFGKVAVLIAVVLVASAPVRAGDRDNLQLYREVQREVLRYPHFT
                     IFDSVHIGVDKGRVTLAGKVTMPFKRTEIARRVAALPSVTSVDNRIEELPVSQSDNEL
                     RYLIARAIYGNQNFRAYASRVNPPIHIIVERGRVTLEGVVLSNVDRMLARSIASSFLA
                     FSVKNDLKTEAEARDALEEL"
     gene            complement(4698..5429)
                     /locus_tag="AC16CY_05595"
     CDS             complement(4698..5429)
                     /locus_tag="AC16CY_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3353289.1"
                     /translation="MPIPLVALGLSLLGGLGGLLVASGVLLINDTVRARLVPWLVSYA
                     VGALLGVATLAILPSALEQLPATRVLGTLLLGILIFFLLEKLVLWRHCHTHDCEVHDG
                     AVLPVLVGDAFHNFVDGAVVAAAVLTSVPLGVSTAIAVAAHEIPQELGDVAILLHAGY
                     SRRKAMLLNALSALSSGLGAVAALLSFDLLPQLLPYFLALAAASFLYVAMADLIPGLH
                     RGRTDAGSMRQILLIGLGIASILVL"
     gene            complement(5469..6494)
                     /locus_tag="AC16CY_05600"
     CDS             complement(5469..6494)
                     /locus_tag="AC16CY_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3353290.1"
                     /translation="MKGLLWTIGLVLAAAVAIGAQSQEGTLFRSGVDFVSVTATVTND
                     NGRFVSGLRRQDFTVYDDGVVQDVSHFSSERVPVSLGILLDVSGSMSVEKLATARRAI
                     ERLIFDLLEPDDELFFMQFATRAELVERWTTDRERIRRALRRMDASDGTALYDAVAAA
                     IPTASEGRHRKKALLVISDGNDTSSTMNVPLLRQRILDSDVLVYALGVDSTARTDTRV
                     PIVLPQIQFPTPFPRPGGFPPQQPRFPPPIVGGGSSTWPSSPGERVNADVLRQITDDT
                     GGRTEIVRGFKGLDDATARIADELSKQYFLGYTSSVPRDGRWHSIRVTVRKGRVNVRA
                     RRGYFAS"
     gene            complement(6605..>6882)
                     /locus_tag="AC16CY_05605"
     CDS             complement(6605..>6882)
                     /locus_tag="AC16CY_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353291.1"
                     /translation="VVHAGDAQAAAGVPRAGVLGECRRVLRSGGRIVVLEAGTRTGLA
                     GIFRAREPREAGATSQELQAAGFQAVRILGDRDGCRFIEGLKVDLQK"
BASE COUNT         1179 a         2260 c         2303 g         1140 t
ORIGIN      
        1 gaaaagacca cgccgggccg gagcaccttc gacgtcagaa tcggcgacac gaattggccg
       61 tgccagaaac caccatgggg ccggctgacg gcgatcgaca cgtcgaccgg ggaattcgcc
      121 tggcaagtcc cgctcggcat caccgagcag ctgcctgcgg gaaagcagaa tacggggcgg
      181 cctgtgctgg cgggtccaat cgtgaccgcc agcggactcg tgttcatcgc ctcgaccgac
      241 gacaaccgct tccgggcgct cgacgccaag acgggcaagg agctctgggt gatgcggctc
      301 gagcggagag gcaacgccaa cccgatcacg tatcagggca cggacgggcg gcagtacgtg
      361 gcagtcgtcg cgaccgacac gttgatggtc tacgcactcc cgaaatgaac cacacgtccg
      421 cggccgaacc tgaacacgaa ccgagaagtg agaagcgaga agtgtgaacg cacggttcac
      481 tatgccttcg cgtcgcgcca gttctccccg attcccacat cgactgtgag cggcactgcc
      541 agtgaggccg cgctttccat ccgctcgcgt acgagcgctg cagccgattc cgcttcctcc
      601 tttgggcact cgaagaggag ctcgtcgtgt accgtcagga tcatgcgcgc gcggaacccc
      661 tggcgcggca atgccgcatg caggtcgatc atcgccttct tgaggatgtc ggccgcggtc
      721 ccctgaatcg gcatgttgac cgtttcgcgc tcggcctggc tccgtacctg gaagttgcgg
      781 ctcgtgaggt tcggggtgag ccgacgccgg ccgaacatcg tcttgacgac accggtccgg
      841 cgcgcctctt cgagcgtgtg gtcgatgaat tctcgaacgc gcggatagcc cgcgaagtat
      901 gcgtcgatga atgcctgggc ggcttcctgt gtgacgccga tgtccttggc cagcgtgaac
      961 tgtgtcttcc cgtacagcac cgcgtagttg accatcttgg accggctgcg caactcgtgc
     1021 cggctgagcc cgctgtcggt tccgaacaac ttcgccgcgg tccgatcgtg aatgtcttcg
     1081 ccggcccgaa aggcctcgat gagggcgtct tcgccggcca gatgagccag cacgcgcagc
     1141 tcgatctgcg agtagtccgc ggagatcagc acgtgtccag gcgcggcaat gaacgcgcgt
     1201 cggatgtcgc gtccgtgttc ggtccggatc gggatgttct gcagattggg gtcggagctg
     1261 ctgaggcgtc ccgtggcggc aaccgcctga ttgaagctgg tgtgcacgcg gcccgtcgcc
     1321 ggattcacca gcagcggcag cgcgtcgatg taggtgctct tcagtttctg gagcgcgcgc
     1381 cagtcgatca cgagccgcgg cagctcgtga cccgtcgcga gctcctccag cacctcggcg
     1441 gcggtcgagg ccgagcgtgt cttgcccgtc ttcctggcca ctggcagcgc cagcttctcg
     1501 aagaggatcc gtccgagctg ctggggcgag ttgatgttga attcctcgcc ggccagctcg
     1561 tagatgcgcg cggtgaacga cgccagctcc tgctcgaagt gcctcgattg cgcggcgagc
     1621 agcggtgtgt cgatgagcac gcccgcctgc tccacgtgcg ccagaaccgg gatgagcggc
     1681 atctcgagat cacggaagac cggctcgagc tgttcggaca cgagctgagg ggccagcttg
     1741 cccgacagct gccaggcgag atcggcgcgc tctccggcaa agccgagcag gttctccggc
     1801 gacacctgcg agaaggcaac cgcgcgcgcg ccgcgtcccc gtacatcgtc cgccgtcctc
     1861 gcccggtagc cgaggtgctg gagcgccgcg tcctcgagat cgtgactggg ccgggtcgca
     1921 tcgagcaggt agctcgccag catggagtcg aacgccaggc cacgcagggt gatgccctga
     1981 cgcgacagga tcaggctgct gcccttgaga tcgtgcccgc gctttgcgag cgtctcgtct
     2041 tcgaggagcg gtccgagtcg atcgaagacc agcccccgat ccagctgcgt cggtgcctcc
     2101 gcgctggcca ggaggcttcc gccagccgtg tctcccgcgt gcccgaccgg gacgtaacgg
     2161 gcccaccggt cggcggccga cacggcgatg cccacgacgc cggcctgcac ggctgatgcg
     2221 tgatcggcga tcacatggat cgccatgaca ccggtggacc gcaggtgagc agccagggcg
     2281 tcgagctcag gctcagtcgc aacgacgccg tagtcggcct ggaccgaact ggcgtcgggc
     2341 gcgtactcgt tgacgagcgt ccggaaatcg agccgagtga acagctcgta gcagcggtcc
     2401 ttggacgcgc ctcgatagcg aagtgtctcg aagtcgggct cgagggggac atccgtgcgt
     2461 atcgtgacca gctcgcggct ccgccgtgcg acgtcgcgat gggactcgag cgcctcgcgc
     2521 tgttttccct tcaactctga cgcgtgcgcg agcagtccgt cgaggctgcc gaactgtgtc
     2581 accaggtcga tcgcgccttt cttgccgatt ccgggcacgc cggccacgtt gtcgctcgaa
     2641 tcgcccacga gggcgaggac gtccacgacc tgcgtcggct tcacgccgaa tttctcggcc
     2701 acggcctgct cgtcgaacca ggccccgtcc tcgcgtgggt cgtacacgcg aacggacccg
     2761 tcgcggacga gttggaagaa gtccttgtcg atcgagacga tcgccacctg gtaccccgcc
     2821 gccgccgccc gtgacgccac cgtgccgatc acgtcgtcgg cttcgtagcc ggggaacgac
     2881 aggatgggca cccccatcgc ttcgcaggct tcatggaccc agttgatttg ttccacgagg
     2941 tcatcgggca tctcagcccg gttggccttg tagtcggtcg cgatctcgtc gcgaaaggtg
     3001 gggccgggaa gatcgaacga cgcggcgagg tacgccggtt tgtgatccgc gatcaacttc
     3061 cgcagcatcg tcacgaagac gtagaccgcg tgcgtggaac gaccttcctg gttcgagagc
     3121 ccgcggccac gaaacgcgtg ataggcccgg tacatctggg aactgccgtc gatgaggaac
     3181 agcgtcggac gcgccatgcg cccacgataa tattcctttg atggttcccg cacggttccg
     3241 cgtcgcgctg agcgcggccg cctgcgcgac ggtcgcgtgg tcctgcggca gcgggcttct
     3301 gggcacgcag tacgaatacg aggagcagat acgcctctcg gtggacggct ccgcgcgcgt
     3361 ggacgtcagc tcgtcggtgc ccgccctcgt cgcgctgcga ggggtggacc tcccgctcga
     3421 tccggccgct gaggtcgatc ggtcgaagat ccggtcgtta ttcgaaggcc cgggcgcgac
     3481 tgtcgcgtcg gttcgccggt cgaggcgcgc cgggcggcag ttcgtccacg tgcgcgtcga
     3541 ggtggacgac atccgcggcc tttcgagaat cgcgccgttt tcgtggtcga cctatcagct
     3601 caaccgccga ggagacgtgc tcgagtacgc gcagaccgtc ggcgcatcgt cagggaaggg
     3661 cagcagcgag gccgcctgga ccggaaacga agtggtcgcc ttccgcatgc acatcccgag
     3721 cgtcatcgtc tttcacaatg ctccgtcgag gaaggtcagg cgggggaaca tcgtcgagtg
     3781 ggaacagccg cttgcagagc gcctgaagaa cacgcctgtc gacattcgcg tcaacatgga
     3841 ggcggagtcg attctctata cgacgctgct cctcttcggg tcgacgatcg tcctggcggc
     3901 catcgccttt ggcgtcgtga tctggcgcgt ggcgaggcac ggtcgtcgca acccgccgaa
     3961 gcccgcgatt cccgcggcca gctgacacgc cgttcagagt tcttccagcg cgtctcgagc
     4021 ttcagcctcg gtcttcaggt cgttcttcac gctgaaggcg aggaacgagc tggcaatcga
     4081 gcgcgccagc atcctgtcca cgttgctgag cacgacgcct tccagcgtga cgcgcccgcg
     4141 ctcgacgatg atgtggatgg ggggattgac gcgcgaggcg taggcccgga agttctggtt
     4201 gccgtagatg gcccgcgcga tgaggtaacg cagctcgttg tcggattgcg agaccggcag
     4261 ttcctcgatc cggttgtcga cgctggtgac cgacgggagg gcggccacac gccgcgcgat
     4321 ctccgtgcgc ttgaacggca tggtcacttt gcccgcgagc gtgacgcgcc ccttgtcaac
     4381 cccgatgtgc acgctgtcga aaatcgtgaa gtgggggtac cgcagcacct ctcgttggac
     4441 ttcccggtag agctgcaggt tgtcgcgatc acccgcacga actggtgcgc tcgccacgag
     4501 caccacggca atcagcacgg cgactttccc aaacatggct tgccccttct cttgcagccg
     4561 gctgctggca gcccgccgcc agctggtaaa ggcaagggct ttgccaaccc cggtcaaccc
     4621 ggctgggcac gatcctgggg atgtggctgc ctcaggtgtc gtcgcgcggg gacaccggcc
     4681 gcagcaacga ccgcgtgcta gagaacgaga atcgatgcga tccccagacc gatcagcagg
     4741 atctggcgca tcgagccggc gtcggtacgg ccgcggtgga ggcccgggat gaggtcggcc
     4801 atcgccacgt agaggaaact ggcggcggca agggccagga aatacggaag gagctgtggc
     4861 agcaggtcga aagacagcag cgcggcgacc gccccgaggc cgctcgacag cgccgacagc
     4921 gcgttgagca gcatcgcctt gcggcgcgaa tagccggcat gcagcaagat cgcgacgtca
     4981 ccgagctcct gcggaatctc gtgagccgcg acagcgattg cggtactgac gccgagcggc
     5041 accgacgtca acacggcggc ggcaacgact gcgccgtcca cgaagttgtg aaaggcgtcg
     5101 ccgacgagca ccggaagcac cgcgccgtcg tggacctcgc agtcgtgagt gtggcaatga
     5161 cgccagagta cgagcttctc gagaagaaag aagatcagga tgcccagcag caacgtcccg
     5221 agcacgcgag tcgccggaag ctgctcgagc gccgacggaa gaatggcgag cgtcgccacg
     5281 cccagcagcg cgccgacggc gtagctcacg agccacggaa ccaggcgggc gcggaccgtg
     5341 tcgttgatca ggaggacgcc ggacgcgaca agcaggccgc cgagcccgcc cagcaagctc
     5401 agtccgagag cgacgagagg aatcggcacc gagtgtgatg atatccactc gaccgtttcc
     5461 gtccaacctt acgatgcgaa gtagccgcgc ctcgctcgaa cgttcacgcg gcccttgcgc
     5521 acggtgacgc gaatggagtg ccagcggccg tctcggggaa ccgagctggt gtagccgagg
     5581 aaatactgct tgctcaactc gtcggcgatg cgcgcggtcg cgtcgtcgag gcccttgaac
     5641 ccgcgcacga tctccgtgcg gccgccggtg tcgtcggtga tctggcgaag cacgtcggcg
     5701 ttgacgcgct cgccggggct gctgggccac gtggagctgc cgccgcccac gatcggcgga
     5761 ggaaaacgcg gttgctgggg cgggaatccg ccgggccgcg gaaacggcgt cgggaactga
     5821 atctgcggca gcacgatcgg aacgcgcgta tccgtgcgcg ccgtcgagtc cactccgagc
     5881 gcgtagacga ggacgtcgct gtccagaatc cgctgacgaa gcaggggcac gttcatcgtg
     5941 ctgctcgtgt cgttcccgtc ggaaatcacg agcagcgcct tctttcgatg ccggccctcc
     6001 gacgccgttg gaattgcagc ggccacggcg tcgtacaggg ctgttccatc cgacgcatcc
     6061 atccttctca gggcacgccg tatgcgctcg cgatccgtcg tccagcgctc gacgagctcg
     6121 gcacgcgtcg caaactgcat gaagaacagc tcgtcgtccg gctcgagaag atcgaagatg
     6181 agccgctcga tcgctctgcg cgcggttgcc agtttctcga ccgacatgct gccgctgacg
     6241 tcgaggagga tgccaagact gacgggcacg cgctcgctgc tgaaatggga gacgtcctgg
     6301 accacgccgt cgtcgtagac ggtgaagtcc tgccggcgca ggcccgagac gaagcgcccg
     6361 ttgtcgttgg tgaccgtcgc cgtgacgctg acgaagtcga cgccgctccg gaagagcgtc
     6421 ccttcctgcg actgcgcccc gatcgccacg gccgcggcca ggacgagccc gatggtccag
     6481 agcagcccct tcatcagtag atcaccatac aggcccgccg gcgtcagggc aaatcccttg
     6541 ccttcgagac cggcccggat caggcgcgaa ctggcaggga ttgctccccc aggagcctgt
     6601 gttgtcactt ctgtaggtca accttcagtc cttcgatgaa acggcagccg tcgcgatccc
     6661 cgaggattcg gaccgcctgg aaccccgccg cttggagttc ctgggaggtg gcgccggcct
     6721 ccctcggctc ccgggcgcgg aatatccccg caagtccggt gcgcgtccct gcttcgagca
     6781 caacgatacg cccgccggag cgtaggacgc gcctgcactc gccaagtact cccgcgcgcg
     6841 ggacgccggc cgcggcctgg gcgtcgcccg catggaccac gg
//
LOCUS       DBNGSH010000244         4798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_188797, whole genome shotgun sequence.
ACCESSION   DBNGSH010000244 DBNGSH010000000
VERSION     DBNGSH010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_188797"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="AC16CY_05610"
     CDS             <1..339
                     /locus_tag="AC16CY_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353292.1"
                     /translation="DFATDTTVTPESPTGGRGLQPSPATALQENTTGYVDHVDAAGNI
                     VGNGVNPPNTAVYTRRWSIEPLPTNPNNTLIIQVLVTPNRVRGQANQGTVSRLVGEAR
                     VITVKTRKAQ"
     gene            336..1547
                     /locus_tag="AC16CY_05615"
     CDS             336..1547
                     /locus_tag="AC16CY_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR3353293.1"
                     /translation="MTDRGIGRRVRGDAGFTLMELLVSSAIMVLITGAIFSMMNPAQG
                     AYKVQPEVSDLQQRLRVGIDTLQKDLVMAGAGTYSGPAAGALSYFIAPVMPYNAFSSP
                     TDPAQGVYFRPDMISLLYVPPTPSQTTLSDPMPQPSAELKVNPQPNCPGNLLNQLCGF
                     EEGDRLIIFDPSGNWDTFTVTEVQNAAAHLQHNQQSFTAAYQVGANVTAIQAAVYYLL
                     SDDATNTHQLRYFDGWNTDLPVVDNVVRLQFQYFGDPQPPQLTGKPLTSKPGPWTTYG
                     PSPPELGISYAGWPPGENCTFQVVGGQQVPRLATLGGGGIGQVELTQAMLSDGPWCPE
                     AGKPSRFDADLLRVRRVRVTIRVQAALASLRGPAGTMFLKGGTANASDRFIPDQEIQF
                     DVTPRNMNLGR"
     gene            1549..2343
                     /locus_tag="AC16CY_05620"
     CDS             1549..2343
                     /locus_tag="AC16CY_05620"
                     /inference="COORDINATES: protein motif:HMM:NF025699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HIR3353294.1"
                     /translation="MQTASTRTSIGSRLKDEQGIALIVALLSMMLLTALGMALSLTTT
                     TERKISTNYRDGVETLYAADAAVERVMQDVLTVPDWNRIVDGTVTSSFVDGAPGTRTL
                     PNGAQLNLLEATAMARCGKLSCSPADMDAYTNERPWGPNNPRWQLYAYGKLEDIIQTQ
                     TINSSVYVIVWMADDPSDNDDNPLLDGGTTNGANPGTGVLSMMAYAYGPTGARRVIET
                     TVARTDTTEIERGYTGQRGQDEQNRRARKAAVQTPGKALTRTVLSF"
     gene            2360..>4798
                     /locus_tag="AC16CY_05625"
     CDS             2360..>4798
                     /locus_tag="AC16CY_05625"
                     /inference="COORDINATES: protein motif:HMM:NF017388.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HIR3353295.1"
                     /translation="MTTIFRRGVLGIVLAGSLFSPREAAAQLDPLLYLKGNKPTVLIA
                     VETANRMQRDTDGNYRDDNIYVRLMGANQVWEDALGIDNGNTFQFYRRKFVGLINTDS
                     SVSGDRFAADHIEIVGDRETAYGTFDQATRVSIARNSLKEAINRNSGVVRFGLLRTRQ
                     KSPRFETPAPAGAVKWSINEGPVTILNGAWLQQQLTGDINANRWKITRPIVDDVNGAI
                     AGPVGPLVAPDSNASSVVVGTSIVNILNLPTGSVGSLLPSGRDQFAVVDAPVDNMLDD
                     LRAEAARLIAADPECRNTVAILVVGGGEGNTSGENPVAKATSFLNVSGRRVPIHVIAI
                     APLSAADRTQLQDVASNSGGVFTEITAAMVQATPLGQPVPEFVRAVNFAVSHTFADQG
                     DFNLPPDATHPYGFSTNHQVTSPIVGTVNLENGVDINGSALPDTIITHPVTGTVIPQR
                     SNIMITTGFSLPGFEAKVRAVRVYRPVVDPSKSVGYKFVQDGTRLWDASAPAAASRNI
                     YTALPNGTMVAFTPANAAILRAYLKTIDVASAASLIDYIRSQPLGAIVGSTPAIMDAP
                     SLDPPPDADYPGFADANKDRRSIIWFGGNDGMVHAVDARLGKEVWAFIPFNLLPKLNA
                     LRNGQPVGDFRYYVDASPKIADVKVGGQWKTYMVIGQGPGGVFYQTFDVTLPNMANTV
                     MPESDAIADVLSYFSTTASVPLKWAFPQYSHFDVNIGAWGDIAAAAPAISKTVGETWS
                     DPAVGQVGTASSPFVVLTGSGFFKRSVQMQANRGGVVAGNVFYMLDAATGLVLDSRSV
                     GSDGKAETVDNCAAI"
BASE COUNT          880 a         1618 c         1540 g          760 t
ORIGIN      
        1 gatttcgcga cggacacgac tgtgacgccg gaatcaccga ccggtggccg gggactgcag
       61 ccttcgccgg ccaccgcgct gcaggagaac acgacggggt acgtcgatca cgtggatgcc
      121 gccggaaaca tcgtcggcaa cggcgtcaac ccgcccaata cggcggtcta cacgcggcgc
      181 tggtcgatcg agccgctgcc gacgaacccg aacaacacgc tgatcatcca ggtgctggtg
      241 acgcccaacc gagtcagagg ccaggcaaat caaggcacgg tgagtcggct cgtgggcgag
      301 gcgcgagtga tcaccgtcaa gacgaggaaa gcgcaatgac cgatcgcggc atcggacgac
      361 gcgtgcgggg agacgcgggg ttcacgctca tggagctcct cgtgtcctcg gccatcatgg
      421 tcctcatcac cggggcgatc ttctcgatga tgaacccggc tcagggcgct tacaaggtgc
      481 agcccgaggt gtccgatctg cagcagcgtc tgcgagtggg catcgacacg ctgcagaagg
      541 acctggtgat ggcgggagcc gggacctaca gtggccctgc ggccggggcg ctcagctact
      601 tcatcgcccc ggtcatgccg tacaacgcct tcagctcgcc caccgacccc gcgcagggtg
      661 tctacttccg gccagacatg atcagcctgc tctacgtgcc gccgaccccg tcgcagacga
      721 cactctcgga cccgatgccg cagccgtcgg ccgagctcaa ggtcaacccg cagccgaact
      781 gtccgggcaa cctgctgaac cagttgtgcg gcttcgagga aggcgatcga ttgatcatct
      841 tcgatcccag cggcaactgg gacacgttta cggtcacgga agtgcagaac gcagcagccc
      901 acctgcagca caatcagcag agcttcacgg ctgcctacca ggtcggtgcg aacgtcacgg
      961 ccatccaggc ggccgtgtac tacctgctca gcgacgatgc gaccaacacc caccagctcc
     1021 ggtatttcga cggctggaac accgatctgc cggtcgtcga caacgtggtc cggctccagt
     1081 tccagtactt cggggatccc cagcctccac aactgaccgg caagccgctc accagcaaac
     1141 ccggcccatg gacgacgtat ggcccgtcgc ctcccgagct tggcatctcg tacgctggct
     1201 ggcccccggg agagaactgc acctttcaag tcgtgggcgg ccagcaggtg ccccggctcg
     1261 ccacgctcgg cgggggcggc atcgggcaag tggaactgac gcaggcgatg ttgtcggacg
     1321 gtccgtggtg cccggaggcg ggcaaaccga gccggttcga tgcggatctg cttcgcgtcc
     1381 gacgcgtgcg cgtcacgatc cgcgtgcagg cggcgctcgc ctcgctgcgc ggcccggcgg
     1441 gcacgatgtt cctgaagggc ggcacggcca acgccagtga ccggttcatt cccgatcagg
     1501 aaatccagtt cgacgtgacg ccgcgcaaca tgaatctggg acgctgacat gcagactgcc
     1561 tctacgcgaa cgtcgatcgg gtcacggctg aaggacgagc agggcatcgc gctgatcgtg
     1621 gcgctgctgt cgatgatgct gctgactgct cttggcatgg cgctctcgct cacgacgacg
     1681 accgagcgaa agatttcgac caactatcgc gacggcgtgg aaacgctcta cgcggccgat
     1741 gcggcggtcg agcgcgtgat gcaggacgtg ctgaccgttc cggactggaa ccggattgtc
     1801 gacggcaccg tgacctcgtc gttcgtcgat ggagcaccgg ggacacgcac ccttcccaac
     1861 ggcgcgcagc tgaatctgct ggaagcgacc gcgatggcgc gctgcggcaa gctgtcgtgc
     1921 tctcctgccg acatggacgc ctacaccaac gagcggccgt ggggcccgaa caatccccgg
     1981 tggcagctct acgcctacgg aaagctggag gacatcatcc agacgcagac gatcaactcg
     2041 agcgtctacg tcatcgtgtg gatggccgac gacccctccg acaatgacga caacccgctc
     2101 ctggacggcg gcaccacgaa cggagcgaac cctggtaccg gtgtgctctc gatgatggcc
     2161 tacgcgtatg gcccgaccgg cgcccgccgt gtgatcgaga cgaccgttgc gcgcacggat
     2221 acgacggaaa tcgagcgcgg gtacacgggc cagcgcggcc aggacgaaca gaaccgccgc
     2281 gcgcgcaagg cggcggtgca gacacccgga aaggcgctca ccagaacagt cctgtcgttc
     2341 taaacgctgg accacactta tgacaaccat ctttcgacgc ggagtgctcg gtatcgtcct
     2401 ggcaggctcg ctgttctcgc cccgcgaggc ggcggcgcag ctcgacccgc tgttgtacct
     2461 gaagggcaac aagccgaccg tcctgattgc cgtcgagacg gccaaccgca tgcagcgcga
     2521 caccgacggc aactaccgcg acgacaacat ctacgtgcgg ctcatgggcg ccaatcaggt
     2581 gtgggaggac gcgctcggca tcgacaacgg gaacacgttc cagttctacc gccgcaagtt
     2641 cgtcgggctc atcaacacgg actcctcggt gtccggagac cggttcgcgg cggaccacat
     2701 cgagatcgtc ggcgaccgtg agaccgcgta cgggaccttc gaccaggcca cccgggtctc
     2761 gatcgcgcgc aactcgctga aggaggcgat caaccgcaat tccggcgtcg tccggttcgg
     2821 cctgctccgc acgcggcaga agtcgccccg cttcgaaacg ccagcccctg ccggcgcggt
     2881 gaaatggagc atcaacgagg gcccggtgac gatcctcaac ggcgcctggc tgcagcagca
     2941 gctcaccggc gacatcaacg cgaaccggtg gaagatcacc cgtccgatcg tcgacgacgt
     3001 caacggcgcg attgccggcc ctgtgggtcc gctcgtggcc cccgattcca acgccagctc
     3061 ggtcgtggtg gggacgtcca tcgtcaacat tctcaatctc ccgaccggct ccgtcggctc
     3121 gctgctgccc tccggccgcg atcagtttgc cgtggttgac gcgccggtcg acaacatgct
     3181 cgacgacctc agggcggagg ccgcccggct gatcgccgcc gatccggagt gccgcaacac
     3241 ggttgcgatt ctggtcgtgg ggggcggcga aggcaacaca tcgggcgaga accccgtcgc
     3301 gaaggcgacg agcttcctca acgtgtccgg acgacgggtg cccattcatg tgatcgcgat
     3361 cgcaccgctc agcgccgccg atcgcacgca gcttcaggac gtggcgagca acagcggcgg
     3421 cgtgttcacc gagatcaccg cggcgatggt gcaggccacg cccctcggtc agcccgtgcc
     3481 cgagttcgtg cgcgccgtga acttcgccgt ctcgcacacg ttcgccgatc agggggactt
     3541 caacctccca cccgacgcca cgcatccgta tggtttctcg acgaaccatc aagtgacgag
     3601 cccgatcgtc ggaacggtca acctcgaaaa cggggtggac atcaacggga gcgcgctgcc
     3661 cgacacgatc atcacgcacc cggtgacggg tactgtcatc ccgcagcggt ccaacatcat
     3721 gatcacgacc ggcttctcgc tgccgggatt cgaggccaag gtgagagccg tgcgcgtcta
     3781 ccggcctgtc gttgatcctt ccaagtccgt cgggtacaag ttcgtccagg acggcacgcg
     3841 gctgtgggat gcgtcggcgc ctgccgccgc gtcgcgaaac atctacacgg cgctgccgaa
     3901 cgggacgatg gtcgcgttca cccccgcgaa cgccgccatc ctgagggcgt acctgaagac
     3961 gatcgacgtg gcgtccgccg cgtcgctcat cgactacatc cggtcgcagc cccttggcgc
     4021 gatcgtcggt tcgacgccgg cgatcatgga tgcgccgtcg ctcgatccgc ctcccgacgc
     4081 cgactacccg ggattcgcgg acgcgaacaa ggatcgccgc agcatcatct ggttcggcgg
     4141 caacgacggc atggtgcacg cggttgacgc ccggctgggg aaggaagtgt gggcgttcat
     4201 tcccttcaac ctgctgccga agctgaacgc gctcaggaac gggcagccgg tcggcgactt
     4261 ccggtactac gtcgacgcct cgccgaaaat cgcggacgtc aaggtgggcg ggcagtggaa
     4321 gacgtacatg gtgatagggc agggtccggg cggggtcttc tatcagacgt ttgacgtgac
     4381 gctgcccaac atggcgaaca cggtcatgcc cgagagcgac gcgatcgccg acgtgctgtc
     4441 gtacttctcc acgacggcca gcgttccgct caagtgggcc tttccgcagt attcgcactt
     4501 cgacgtgaac atcggtgcgt ggggcgacat cgcggcggcc gctcccgcca tttccaagac
     4561 cgtcggtgaa acgtggtcgg atcctgccgt gggccaggtc ggcacggcct cgtcgccctt
     4621 cgtcgtcctg accgggtccg gcttcttcaa gaggtcggtg cagatgcagg ccaatcgcgg
     4681 cggcgtcgtg gccggcaacg tcttctacat gctcgacgcc gcgaccggcc tggtgctgga
     4741 ctcccggagc gtcggcagcg acggcaaagc ggagaccgtg gacaactgcg ccgccatc
//
LOCUS       DBNGSH010000245         3719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3244953, whole genome shotgun sequence.
ACCESSION   DBNGSH010000245 DBNGSH010000000
VERSION     DBNGSH010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3244953"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="AC16CY_05630"
     CDS             complement(<1..680)
                     /locus_tag="AC16CY_05630"
                     /inference="COORDINATES: protein motif:HMM:NF016593.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353296.1"
                     /translation="MPPLVEELVPAPDPVRSCERLDGLPYRLLLDSAIADARLGRYSF
                     LTADPVAVVRSKGGEVECLDLVTSTRRTERGDPLAVLRALVEPYSTPAVAGLPPFQGG
                     VAGYVAYDWGLTLERLPAPRYDDLALPDVVMAVYDWVLAWDHASGQAWLISTGLPETT
                     PSARASRAVMRAAAVKERIRHAHASPLPPAPLPPASSHPPAFAASRLRRGKRPFAPSH
                     LVQPVWWPA"
     gene            complement(680..2134)
                     /locus_tag="AC16CY_05635"
     CDS             complement(680..2134)
                     /locus_tag="AC16CY_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HIR3353297.1"
                     /translation="MPEGTPRRVLFVTGRLAEPSLRRVLEDMGPAFVCDVAVLKITVA
                     ALMTTSWIARHLAVPANTDLVLIPGLCEGDAAVVADAFGVNVEKGPIDLREIPRHFGR
                     PAATREYGARDIDIIAEINNAPRLTREAIRREAEYYRGCGADVIDIGCTPGLPFPALA
                     DVVRELVDAGMRVSVDTFDADEIRTAVRAGASLVLSVNGSNVDVVQELAASGARWVVI
                     PDPGGPLETLEPTLAKLEAWGAPYLIDPILEPIGLGFMASLERYAEVRRTWPGAEMMM
                     GIGNVTELTAADSTGVNAVLVAICQELGIRAVLTTEVIPWARGAVREIDVARRLMHYA
                     VNGRTIPKGVDDRLVTVKDPAVLTYSEAELRQLQAAITDPNYRIFADREAITVFNSER
                     FVRGTDIQDIFARLGVAEPNHAFYLGKELAKASLAVALGKTYRQEGQLAWGYLTPPED
                     RSGHVRLTHAERAGSDPSTGSGSSPQSRSRSEKP"
     gene            complement(2134..2496)
                     /locus_tag="AC16CY_05640"
     CDS             complement(2134..2496)
                     /locus_tag="AC16CY_05640"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR3353298.1"
                     /translation="MSKKEQTYDEAQIAEKLRELPGWYLEDGWIRRVYKTDGWPTTLM
                     LVNAIGYLSEAAYHHPDLSVTWGRITVKLSTHSAGGITDKDFSLARRIEDAVLWRPAP
                     GEALEGTPNKFVRSGDPR"
     gene            complement(2632..3402)
                     /locus_tag="AC16CY_05645"
     CDS             complement(2632..3402)
                     /locus_tag="AC16CY_05645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3353299.1"
                     /translation="MTESSAPLAGCGVLVTGASSGIGKAIALACARAGADVAITYKAN
                     AAGARDTEAQIRALGRRSALFQVELADERALGALGPAAREALGSIDVWINNAGADILT
                     GPAASLSSVAKMDLLLAVDLRGTIIASWCAADMLSAQPGGGVIINVSWDHVLTGMAGT
                     NPQLFAAAKGGILAFSKSLARSVAPRVRVNVIAPGWIETSFGARMDEAQRQAVAQSTP
                     LERWGTPDDVAGAAVFLASPVAAFITGQVILVGGGVVM"
     gene            complement(3428..>3719)
                     /locus_tag="AC16CY_05650"
     CDS             complement(3428..>3719)
                     /locus_tag="AC16CY_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353300.1"
                     /translation="LSLTTTYDMTAIPMSLAFPLASGALLSIIAFRFGLLALVVTWFT
                     WGLIEAVPMTLQLSHWRAVPSNWTLAVLVGLTLYGFYASRAGQPLLGRILRD"
BASE COUNT          604 a         1290 c         1258 g          567 t
ORIGIN      
        1 gctggccacc agacaggctg cacgaggtgc gagggtgcga aggggcgctt gcctcgccga
       61 agccgcgaag cggcgaaggc gggagggtgc gaggaggcag ggggcaaggg ggcagggggc
      121 agggggctgg cgtgtgcgtg tcggatgcgc tccttgactg ccgccgcacg catcaccgca
      181 cgcgatgccc gcgcggaggg cgtcgtctcc ggcaggcccg tggagatcag ccaggcctga
      241 cctgaggcgt ggtcccacgc cagcacccag tcgtagaccg ccatgacgac atcgggcagc
      301 gccagatcgt cgtagcgagg cgcgggcaac cgctcgagcg tcagccccca gtcgtaggcg
      361 acgtaaccgg ccaccccccc ttgaaacgga ggcaggccgg cgacagccgg cgtggaatac
      421 ggttcgacga gcgcgcgcag cacggcgagc ggatcgccgc gctcggtccg ccgcgtgctc
      481 gtgaccaggt cgagacactc gacctcgcct cccttgctgc gcacgacggc cacgggatcc
      541 gccgtgagga acgaatatcg gccgagcctc gcgtcggcaa tcgcgctgtc gagcagcagc
      601 cggtagggca gcccgtcgag gcgttcgcag ctgcgcaccg gatcgggtgc cggcacgagc
      661 tcttcgacga gcggcggcat cacggcttct ccgagcggga gcggctctgc ggcgaggacc
      721 ctgagcctgt cgaagggtcg ctgccggccc gttcggcgtg cgtcaaccgc acgtgcccgg
      781 accgatcttc cggcggcgtg agatatcccc atgccagctg gccttcctgg cggtaggtct
      841 tgccgagcgc cacggccagg ctcgccttcg ccagttcctt gcccaggtag aacgcgtggt
      901 tcggctccgc gacgccgagc cgcgcgaaga tgtcctgaat gtcggtgccg cgcacgaagc
      961 gttcgctgtt gaagacagtg atcgcctccc ggtccgcgaa gatgcggtag ttgggatcgg
     1021 tgatggcggc ctgcagctgg cgcagctccg cctcggagta tgtcagcacg gcggggtcct
     1081 tcacggtcac caggcggtcg tccacgcctt tcgggatcgt gcggccgttc accgcgtagt
     1141 gcatcagccg gcgcgccacg tcgatttcgc ggacggcgcc gcgcgcccag gggatgacct
     1201 cggtcgtcag gacggcgcgg atgccgagct cctggcagat cgcgacgagc accgcgttga
     1261 ccccggtgga gtcggccgcg gtcagctcgg tcacgttccc gatccccatc atcatctcgg
     1321 cccctggcca cgtccttcgg acttcagcgt accgctcgag cgaggccatg aagccgaggc
     1381 cgatcggctc gaggatcgga tcgatcaagt agggtgcgcc ccacgcctcg agcttcgcga
     1441 gcgtcggctc gagcgtctcg agcggtccgc ccggatcggg aatgacgacc caccgggcgc
     1501 cgctcgccgc cagctcctgg acgacatcga cattggagcc gttgacgctc agcaccaggc
     1561 tcgcgcccgc ccgaaccgcg gtccggatct cgtcggcgtc aaacgtgtcc acgctgaccc
     1621 gcatgcccgc gtccacgagc tcgcgcacga cgtcggcgag cgcagggaat ggaaggccgg
     1681 gcgtgcaacc gatgtcgatg acgtcggccc cgcagccgcg gtagtactcc gcctcgcgcc
     1741 ggatcgcctc ccgcgtcagc cggggcgcgt tgttgatctc ggcgatgatg tcgatgtccc
     1801 tggcaccgta ctcgcgcgtg gcggcaggac ggccgaagtg tcgggggatc tcgcgcaggt
     1861 cgattgggcc cttctccacg ttgacgccga acgcgtctgc gacgaccgcg gcatcgccct
     1921 cgcacaagcc cgggatgagg acgaggtccg tgttcgcggg cacggcgagg tggcgcgcga
     1981 tccaggaggt tgtcatcagc gccgccacgg tgatcttgag gaccgccacg tcgcagacga
     2041 aggcgggccc catgtcctcc agcacgcgcc gcagcgacgg ctcggccaga cggcccgtga
     2101 cgaacagcac gcgccgcggc gtaccctccg gcatcagcga ggatcgccgc tccgcacgaa
     2161 cttgttcggc gtcccctcga gcgcctctcc cggagcgggg cgccagagca ccgcgtcctc
     2221 gatccgtcgc gcgagcgaga agtccttgtc cgtgatgccg cctgcgctgt gcgtagacag
     2281 cttcacggtg atccgccccc aggtcaccga cagatcggga tggtggtagg ccgcttcaga
     2341 caggtacccg atcgcgttca cgagcatcag cgtcgtcggc cacccgtcgg tcttgtagac
     2401 gcggcggatc catccgtcct cgagatacca ccccggaagc tcgcgcagct tttcggcgat
     2461 ctgcgcctcg tcgtatgttt gttctttctt cgacataacc tgcaccccca ccacaccacg
     2521 aaggccacga aaatcgcgga gatcacgaaa tcagttctct acacgagaag aactgcttcg
     2581 tggacttcga gatcttcgtg agcttcgtgg tctcgacagc cgtcggagtt attacatcac
     2641 cactccgcca ccgaccagga tgacctgccc cgtaatgaac gctgccaccg gagaagcgag
     2701 gaacacggcg gctccggcga cgtcgtccgg tgtgccccag cgttcgaggg gagtggactg
     2761 cgcgacggcc tggcgctggg cctcgtccat ccgggcgccg aacgacgtct cgatccagcc
     2821 gggggcaatg acgttcaccc gcacgcgcgg ggccaccgat cgcgcgagcg acttgctgaa
     2881 cgccagaatg cctcccttgg ctgcagcgaa cagctgcggg ttggttcccg ccattccggt
     2941 cagcacgtga tcccagctga cgttgatgat cacgcctcct cctggctggg ccgacagcat
     3001 gtcggccgcg caccacgagg cgatgatcgt cccccgcagg tcaaccgcca ggaggaggtc
     3061 catcttcgcc acgctcgaga gcgatgcagc ggggccggtc agaatgtcgg ctccggcgtt
     3121 gttgatccag acgtcgatcg atccgagcgc ctcgcgcgcc gcagggccga gcgccccgag
     3181 ggcccgttcg tccgccagct ccacctgaaa cagagcactc cggcggccaa gcgcccggat
     3241 ttgcgcctcg gtgtcgcgcg cgccggcggc gttggccttg tacgtaatcg ccacgtcggc
     3301 cccggctcgt gcgcacgcga gtgcgatcgc ttttccgatc ccgctcgacg cgcccgtcac
     3361 caggaccccg cagccggcca ggggcgcact cgactccgtc atccggcgat catatgagct
     3421 ttggggctca atccctgaga atccggccca agagcggctg gcccgcgcgc gaggcgtaga
     3481 agccgtacag ggtgagcccg acgagcacgg cgagcgtcca gttcgagggc acagcccgcc
     3541 aatgcgacag ctgcagcgtc atgggaacgg cctcgatgag gccccaggtg aaccacgtca
     3601 ccacgagcgc cagcaatccg aaccggaagg cgatgatcga cagaagcgcg ccgctggcga
     3661 gcgggaatgc gagcgacatc ggtatcgccg tcatgtcata cgttgtcgtc agcgacagg
//
LOCUS       DBNGSH010000246         3678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_868176, whole genome shotgun sequence.
ACCESSION   DBNGSH010000246 DBNGSH010000000
VERSION     DBNGSH010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_868176"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..512
                     /locus_tag="AC16CY_05655"
     CDS             81..512
                     /locus_tag="AC16CY_05655"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015119054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3353301.1"
                     /translation="MTSEIRILGSSDDAILQRVAPDLFDDVLKPALAAEFLRDHRHHL
                     AVAVEEGIVVGFASGVHYVHPDKPPEMWINEVGVAPTHQGRGLGRAVVRALLEHATRL
                     GCREAWVLTDRSNSAAMRLYASAGGEEAPGDHVMFNFVLDR"
     gene            581..976
                     /locus_tag="AC16CY_05660"
     CDS             581..976
                     /locus_tag="AC16CY_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3353302.1"
                     /translation="MSNAETVKAIYEAFGRGDVPTIMEKLDDAVEWETTVPVPGVPWL
                     QARRGKANIVGFFESLAPLEITRFEPHTIFDGGDKVFVLITFEATTRGKSYSFPNNGH
                     LWRFNATGKVVKYDHVTDTAQMIRMAKGE"
     gene            1047..2075
                     /locus_tag="AC16CY_05665"
     CDS             1047..2075
                     /locus_tag="AC16CY_05665"
                     /inference="COORDINATES: protein motif:HMM:NF052887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8021 domain-containing protein"
                     /protein_id="HIR3353303.1"
                     /translation="MRMTGVGWLGFACLLIASSAASAQPRVTPPCDRACLEGYVDRYL
                     DAMLANEVSPKLFSRDVKFTENGIRLPLGNEGLWFGMSGRGRYKFYVPDVDTQQVAFI
                     GTVIENTRARGANTGEGDLVAIGLRLKIVGGLITEVEQLAIRPDTGLGGRGGGGGGAR
                     GAGGAPAAGGAGRTGGPGAGGTGDRVEAMKEPHPVFRETIPEAKRPSREDLIETANYY
                     FTGLARNDGKGYYPFTADCDRFENGIMATKTNCKAQFENNLRGVVSRVRDRRFVAVDR
                     ERGIVFSFAFFDHEQINWTWQLAELFKIENGLIRRIEAVFHRAPYGIPSGWSTFEQAM
                     SESIQSVR"
     gene            2100..2594
                     /locus_tag="AC16CY_05670"
     CDS             2100..2594
                     /locus_tag="AC16CY_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HIR3353304.1"
                     /translation="MGETLASIEHEYRYYKLLAERAIAQLTDQEIAYEAAPESNSVAV
                     IVWHMSGNLTSRFSDFLDSDGEKPWRDRDSEFDPRTVSRDELLRKWNEGWDVLFAALA
                     RLSEKDLERVVRIRNEEHTVIRALHRSVAHASYHVGQIVFIAKLLRGGGWKNLSIPRR
                     PPRA"
     gene            2613..3164
                     /locus_tag="AC16CY_05675"
     CDS             2613..3164
                     /locus_tag="AC16CY_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353305.1"
                     /translation="MALSATRRQSVLPLLASLAFVAIGVLLIREGDRSLMTSAAVVFF
                     GVGAVLFAYLAITGRASLVLVQPTESRVELEPGGFAVLTAKGRRFPVRWASVRRVIAY
                     KRDLWTTDEICVLFESDDGSGRQEVSEEWPGFADLFGVMERELGISPRWYMEIMMPPF
                     EPTPRLLFDRAEHPPRPVEAGGA"
     gene            3191..3604
                     /locus_tag="AC16CY_05680"
     CDS             3191..3604
                     /locus_tag="AC16CY_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353306.1"
                     /translation="MPAALRAVFRRLRTILEAHAGEFHVADDRDGRYGLEGLIGPATL
                     RAWGGKARTGTISVAWVTIGKASVSYHLMGVQGNARLMATVSDELRARMQGKSCFNFK
                     AVDEALFRELARVTADSLQGMRKAGYVAARLKETI"
BASE COUNT          663 a         1128 c         1250 g          637 t
ORIGIN      
        1 cggcatcatc gcgtcggcgt acctctatca ggaggtttca caactctcct gagccgtcac
       61 gagcctgatt cgttttcatc gtgacctctg agatcaggat tctcggttcc agcgacgacg
      121 cgattctgca gcgtgtcgcg cctgacctgt tcgatgacgt cctgaagcca gcgctggccg
      181 ccgagtttct tcgcgatcat cgacaccacc tggctgttgc cgtcgaggag ggcatcgtgg
      241 tcgggttcgc atctggcgtg cactacgtgc atccggacaa accgccggag atgtggatca
      301 acgaagtcgg cgtggcgccg acgcatcagg gccgcggcct cggcagagcc gtcgtgcggg
      361 cgctgctgga gcacgcaacg cgcctgggct gtcgcgaggc gtgggtgctc accgatcgct
      421 cgaattccgc ggcgatgcgt ttgtacgcgt cggccggtgg agaagaagcc cccggcgacc
      481 acgtgatgtt caacttcgtt ttggatcgct gacccgtctg ggctctcagg ttccccacac
      541 agttggggct cattgtcgaa cgaagaggat ggagggcaaa gtgagcaacg cagagacggt
      601 gaaggcgatc tacgaggcct tcggtcgtgg cgatgtaccg acgatcatgg aaaagctgga
      661 cgatgccgtc gagtgggaga caacggtgcc ggtgcctggc gtgccgtggc ttcaagcacg
      721 ccgcggcaag gcgaacatcg tggggttctt cgagtccctg gcaccgctcg agatcacccg
      781 cttcgaacca catacgatct tcgatggggg cgacaaggtc ttcgtgctca tcacgttcga
      841 ggccacgacg cgtggcaaga gctactcctt ccccaataat ggtcacctct ggcggttcaa
      901 cgccaccggt aaggtcgtca agtacgacca cgtgactgat accgctcaga tgatcaggat
      961 ggccaaggga gagtgaccat ggcagctcga cacactggaa ctgcggcgag cacgatagaa
     1021 ttcgcgttcg agcaaggaga agacacatgc ggatgaccgg ggttggatgg ctcggcttcg
     1081 catgcctgct gatcgcgtct tcggccgcgt cggcgcagcc gcgagtgacg ccgccgtgcg
     1141 accgcgcctg ccttgaaggg tacgtggacc gctatctgga cgcgatgctg gcaaacgagg
     1201 tcagcccgaa gctgttttcg cgcgacgtca agttcaccga gaacggcatc cgtctgccgc
     1261 tcggcaacga aggactctgg ttcggcatgt caggacgcgg ccgctacaag ttctacgtcc
     1321 ccgacgtcga cacgcagcag gtcgccttca tcgggaccgt catcgagaac acgcgggccc
     1381 gcggagccaa caccggcgag ggagacctcg tggcgatcgg gctgcggctc aagatcgtgg
     1441 gcgggttgat tacagaagtc gagcagctcg cgatcagacc ggacacgggc ctcggcggcc
     1501 ggggcggtgg aggcggcggc gcccgaggcg cgggcggcgc gcctgcggct ggcggtgccg
     1561 gtcgtacagg cggcccgggt gccggaggaa cgggcgatcg cgtcgaggcg atgaaggaac
     1621 cgcacccggt gtttcgcgaa acgattcccg aggcaaagcg cccgtcgaga gaggatctga
     1681 tcgagacggc gaactactac ttcaccggtc tcgcacgaaa cgacggcaag ggctactacc
     1741 cgttcaccgc cgattgcgat cgcttcgaga acggcatcat ggcgaccaag acgaactgca
     1801 aggcgcagtt cgagaacaac ctgcgcggcg tcgtctcgcg ggtccgcgat cgccgcttcg
     1861 tggccgtcga tcgcgagcgg gggatcgtct tctccttcgc gttcttcgat cacgagcaga
     1921 tcaactggac gtggcagctc gccgagctct tcaagatcga gaacggcctg atccgccgca
     1981 tcgaggcggt cttccatcgg gcgccgtacg ggattccatc gggctggagc acgttcgagc
     2041 aggcgatgtc ggagtcgatt cagagcgtcc gctgatggca cggtgaggag aggcgacttg
     2101 tgggagagac actggcgtcg atcgagcacg agtatcgcta ctacaagctt cttgcggagc
     2161 gcgcgatcgc gcagctgacc gatcaggaga tcgcctacga ggcggcgccc gagtcgaact
     2221 ccgtagccgt catcgtgtgg cacatgtcgg gcaacctcac atcgcgcttt tccgatttcc
     2281 tcgactccga cggcgagaag ccctggcggg accgcgactc ggagttcgac ccgcgcacgg
     2341 tgagccgcga cgagctgctt cggaaatgga acgaaggatg ggacgtgctg ttcgcggccc
     2401 tagccaggct gtcggagaag gacctggaac gggtcgtccg aatccgcaac gaagagcaca
     2461 ccgtgatacg ggcgctccat cggtcggtcg ctcacgcgag ctaccacgtg ggacagatcg
     2521 tcttcatcgc caagctcctt cgcgggggcg gctggaagaa cttgagcata cccaggcgcc
     2581 cgccgcgcgc atgaggccga cggaatcagg gcatggcact gtcggcgacc cggcgtcaga
     2641 gtgtcctgcc gctcctcgcg tcgctcgcct tcgtcgcgat cggcgtcctc ctgattcgcg
     2701 agggcgaccg gtccctgatg acgtcggccg ccgtcgtgtt cttcggcgtc ggcgccgtcc
     2761 tgttcgccta cctcgccatc acggggcggg catccctcgt cctcgtccag ccgacagagt
     2821 cgcgcgtcga actggagcca ggcggcttcg cggtcctcac ggcgaagggc cgccggttcc
     2881 ccgtgcgctg ggccagcgtc cggcgggtca tcgcgtacaa gcgcgatctc tggacgactg
     2941 acgagatttg cgtcctcttc gaatcggacg acggcagtgg caggcaggag gtgtcggagg
     3001 agtggccggg attcgcggac ctgttcggcg tgatggagag agagctcggg atcagcccaa
     3061 ggtggtacat ggagatcatg atgcccccct tcgagccgac gccgcggctg ctcttcgacc
     3121 gggccgagca cccaccgcgt ccggttgaag ccggcggcgc ctgatgagat cctgagccgc
     3181 ccatcgagcc atgcctgcag cacttcgcgc cgtattcagg agactgcgga cgatactcga
     3241 ggcgcacgcc ggcgagttcc acgttgcaga cgacagggac ggccgttacg gtctcgaggg
     3301 cctaattggt cccgcaacgc tccgtgcgtg gggcggcaag gcccggaccg gcaccatatc
     3361 ggtcgcgtgg gtcacgatcg gcaaggcgtc cgtcagctac cacctgatgg gcgttcaggg
     3421 caatgctcgg ctgatggcca ccgtctctga cgagctgcgg gcgcgcatgc agggcaagtc
     3481 gtgcttcaat ttcaaggcgg tggacgaggc gctctttcga gagctggcgc gggtcaccgc
     3541 cgattcgctt caagggatga ggaaagccgg atacgtggcc gctcgcctga aggagactat
     3601 ctgatcgaag agggatgcgg ttcgagcgcc cgaagggacg agcatgactg acgacaccaa
     3661 agcgtgcccc gtgtgcgg
//
LOCUS       DBNGSH010000247         2300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_189010, whole genome shotgun sequence.
ACCESSION   DBNGSH010000247 DBNGSH010000000
VERSION     DBNGSH010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_189010"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16CY_05685"
     CDS             complement(<1..356)
                     /locus_tag="AC16CY_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HIR3353307.1"
                     /translation="MSLSAMKEMSISALTKIAKELEIAGATGMRKQELIFEILRVRAE
                     KSGNVFSEGVLETLPDGFGFLRAPDYNYLPGPDDIYVSPSQIRKFDLHTGDTVSGVIR
                     PPKDGERYFALIKVEA"
     gene            complement(491..1084)
                     /gene="yihA"
                     /locus_tag="AC16CY_05690"
     CDS             complement(491..1084)
                     /gene="yihA"
                     /locus_tag="AC16CY_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR3353308.1"
                     /translation="MSPVVIAEFVTSAASAADFPRDRIPEVAMVGRSNVGKSSLINAL
                     VHQKVARTSAEPGKTRLANIYRVRRGTRPPFYLVDLPGYGYARGGDDSAREFRRLADA
                     YFSRPGTRAVLLLVDARHPGTRSDLEAWTWLLAQQCARAVVGTKVDKLTRAERTRHAR
                     ELETLFELPVALVSARTGEGLDELWKMIANLPNRTAA"
     gene            complement(1176..>2300)
                     /locus_tag="AC16CY_05695"
     CDS             complement(1176..>2300)
                     /locus_tag="AC16CY_05695"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR3353309.1"
                     /translation="DKMSMDFRGDPSAALLEVLDPEQNSTFLDHYLDVEFDLSNVMFI
                     CTANVLHTVPQALRDRMEVLQLAGYTEQEKLEIAKRFLVHKAVEGTGLTENNIIFQDE
                     AIQTIIQRYTREAGVRNLEREISSICRKVARKVVAEDKTFKEEFSAEKVTAYLGVPRF
                     RPSLAEERNEIGIATGLAWTEVGGELLVSEATLMAGKGKLTLTGKLGDVMQESAQAAL
                     SYVRSRAAELNLPEDFHSKIDVHVHVPEGAIPKDGPSAGITMATALTSALTKIPTRRD
                     VAMTGEITLRGKVLPIGGVKEKVLAAHRAGVTNIILPRDNEKDLADIPKNVMDTLDVH
                     MVSTMDEVLKIALAQPLPSRIPAAPVVAEVTEPAGDTRPH"
BASE COUNT          334 a          778 c          762 g          426 t
ORIGIN      
        1 atcgcctcga ccttgatcag cgcgaagtag cgctcgccgt ccttcggcgg ccggatcacg
       61 ccggagaccg tgtcgccggt gtgcaggtcg aacttgcgga tctgggacgg tgacacgtag
      121 atgtcgtccg gcccgggcag gtagttgtag tcgggcgcgc gcaggaagcc gaagccgtcc
      181 ggcagcgtct cgaggacgcc ctccgagaag acgttgccgc tcttctcggc gcgcacgcgg
      241 aggatctcga agatcaactc ctgcttgcgc atgccggtgg cgccggcgat ctccagctcc
      301 ttggcgatct tcgtcagcgc ggagatgctc atctccttca ttgccgacag cgagatcgtc
      361 tgagccgggg cggcctgagc cggggcggcc tgcttgcccg ccgaagcctc ggcggaggcg
      421 ggcgccggca gtgcaccggc gggcggcgcg ccactctttt ctttgtcggg agggggagcg
      481 gtggcggtgc ctacgccgcc gttcggtttg gcaggtttgc gatcatcttc cacagttcgt
      541 ccagtccttc gccggtgcgc gccgaaacga gcgccactgg cagttcaaac aacgtttcca
      601 gttcacgagc atgacgcgtg cgttcggccc gcgtcagttt gtcgactttg gttcctacca
      661 cggcgcgcgc acactgctgt gccagcagcc acgtccaggc ctccaggtcc gacctggtcc
      721 ccgggtggcg cgcgtccacg aggagcagca ccgcgcgcgt gcccggccgg gaaaaatagg
      781 cgtcggccag acgccggaac tcccgcgctg agtcatcgcc gcctctcgcg taaccgtaac
      841 ccggcagatc caccaggtag aacggcggcc tcgtcccgcg ccgcacgcga tagatgttag
      901 ccagcctcgt cttgcccggc tccgcgctgg tgcgggccac cttctggtgc accagcgcgt
      961 tgatcaggct cgacttgccc acgttcgacc gccccaccat ggcgacttcg ggaatccggt
     1021 ctcgcggaaa atcggcagcg ctcgcggcgc tcgtgacgaa ctcggcgata acgacagggc
     1081 tcagggttca ggcctcaggc ctcagcagga ttcggggacc acgcctggcc cggcctcacg
     1141 gagcctgagc cctgggccct gattcctgag gcctcctagt gcgggcgcgt gtcgcccgcc
     1201 ggctccgtga cctccgcgac caccggcgcg gccggaatgc gcgatggcag cggctgggcc
     1261 aacgcgatct tcaacacctc gtccatggtc gataccatgt gcacgtcgag cgtgtccatg
     1321 acgttcttcg ggatgtccgc gaggtccttc tcgttgtcgc gcggcaggat gatgttggtt
     1381 acgccggcgc ggtgcgcggc gagcaccttt tccttgaccc cgccgatcgg caacaccttt
     1441 ccgcgaagcg tgatttcccc cgtcatcgcg acgtcacgcc gcgtggggat cttggtcagc
     1501 gccgaggtca gcgcggtcgc catcgtgatg ccggctgacg ggccatcctt cgggatggcg
     1561 ccctcgggga cgtgcacgtg gacgtcgatc ttggaatgga agtcctcggg caggttcaac
     1621 tctgcggccc gggatcgcac gtagctgagc gcggcctggg ccgactcctg catcacgtca
     1681 ccgagcttgc cggtgagcgt gagcttgccc ttgcccgcca tcagcgtcgc ctcgctcacg
     1741 agcagctcgc ccccgacctc ggtccaggcg agcccggtgg cgattccgat ctcgttccgc
     1801 tcctcggcca ggctcggacg gaaccgaggc acgccgaggt acgcggtgac cttctcggcg
     1861 ctgaactctt ccttgaacgt cttgtcctcg gcgacgacct tgcgggccac cttgcggcag
     1921 atggaagaga tctcgcgctc gaggttgcgg acgccggcct cgcgcgtgta ccgctggatg
     1981 atcgtctgga tcgcctcgtc ctggaagatg atgttgttct cggtcaaccc cgttccctca
     2041 accgccttgt gcacgaggaa gcgcttggcg atctcgagct tctcctgctc ggtgtagccg
     2101 gcgagctgca gcacctccat gcggtcgcgg agggcctggg gaacggtgtg cagcacgttc
     2161 gccgtgcaga tgaacatcac gttcgacagg tcgaactcga cgtcgaggta gtggtccagg
     2221 aacgtgctgt tctgctcggg gtcgagcacc tcgaggagcg ccgccgacgg gtcgccgcgg
     2281 aagtccatcg acatcttgtc
//
LOCUS       DBNGSH010000248         2321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4604462, whole genome shotgun sequence.
ACCESSION   DBNGSH010000248 DBNGSH010000000
VERSION     DBNGSH010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4604462"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /gene="ffh"
                     /locus_tag="AC16CY_05700"
     CDS             <1..1269
                     /gene="ffh"
                     /locus_tag="AC16CY_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR3353310.1"
                     /translation="RVRDKATDQAVLKSLTPVQQVVKIVRDELLALFGEAEGGLTKSA
                     PKPRVVLLLGLQGSGKTTTCGKLGRWLRRQGHHPLLVSTDVRRPAAIQQLAVVAEQAE
                     VKVFAPETLDPVERASGALKEARAKGFDTVIVDTAGRLHIDDELMDELQAIKDAVQPS
                     DLLYVADAMTGQDAIKSAGEFNRRVGVTGVVLTKLDGDARGGAALSVVSVVGVPVAFI
                     GSGERLEDLELFHPDRIVSRILGMGDVLSLIERAEQAIGEDEAEQLEEKLRKNQFTLD
                     DFRTQLKALKRMGPLESVLGMIPGLGSLKQLGENKPDEKQLARIEAIICSMTPAERSN
                     ENLIGGSRRRRIALGSGTTVADVNRLLKQFSDMKRMLQMIGRGGTAALKGMKGMKGMP
                     KLSHQAQAGFAQAGGRKRKKGGPWGLIKSR"
     gene            1303..1575
                     /gene="rpsP"
                     /locus_tag="AC16CY_05705"
     CDS             1303..1575
                     /gene="rpsP"
                     /locus_tag="AC16CY_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR3353311.1"
                     /translation="MLAIRMRRVGAKKKPYFRVVVTEAKSARESTFVENVGTYHPSEK
                     PARVEINKARVQHWLQLGAQPSNSVRTLLARHLSRDLSAPTEAPAQ"
     gene            1623..1853
                     /locus_tag="AC16CY_05710"
     CDS             1623..1853
                     /locus_tag="AC16CY_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR3353312.1"
                     /translation="MRAVVEVVAKALVDRPEQVKVTESVRRGMTVVELTTAPNDMGKI
                     IGRQGRTAAALRTVVAMAAERLGVRAQLDIRD"
     gene            1962..>2321
                     /gene="rimM"
                     /locus_tag="AC16CY_05715"
     CDS             1962..>2321
                     /gene="rimM"
                     /locus_tag="AC16CY_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR3353313.1"
                     /translation="MVLVGRIARPHGLRGQVVVTPETDFAEDRFRAGATLWTRSRRGD
                     EVLTISTARLQNGRPVIGLEGFATIEEVERLAGLELRVPEEALKPLADGAYYVHQLVG
                     CVVETVAGQRVGEVARVE"
BASE COUNT          441 a          670 c          867 g          343 t
ORIGIN      
        1 cgggtccgcg acaaggcgac ggaccaggcg gtgctcaaga gcctgacgcc ggtgcagcag
       61 gtcgtcaaga ttgtcaggga cgaactgctc gcgctgttcg gcgaggcgga aggcgggctg
      121 acgaagagcg cgccgaagcc tcgcgtcgtc ctgctgctcg ggctgcaggg atcgggcaag
      181 acgacgacct gcggcaagct gggccgctgg ctccgtcgtc aagggcacca tccgctgctg
      241 gtatcgaccg acgtcaggcg ccccgcggcg attcagcagc tcgccgtcgt ggccgagcaa
      301 gcagaggtga aggtgttcgc gcccgagacg ctcgacccgg tggagcgcgc gtctggcgcg
      361 ctgaaggagg cgagggcgaa gggcttcgac acggtcatcg tcgatacggc ggggcgcctg
      421 cacatcgacg acgagctgat ggacgagttg caggcgatca aggacgcggt tcagccgtcg
      481 gacctgctgt atgtcgccga tgcgatgacg gggcaggacg ccatcaagag cgctggcgag
      541 ttcaatcgcc gcgtgggtgt gaccggcgtc gtcctgacca agctcgatgg cgacgcgcga
      601 ggtggcgcgg cgctctcggt cgtgtccgtc gtcggggtgc ccgtggcgtt catcggcagc
      661 ggtgagcgcc tcgaagacct cgagctcttc cacccggatc ggatcgtttc gcggattctc
      721 gggatgggcg acgtgctgtc gctcatcgaa cgcgccgagc aggcgatcgg agaagacgag
      781 gccgagcagc tcgaagagaa gctgcggaag aatcagttca cgctcgacga cttccgcacg
      841 cagctcaagg cgctgaagcg gatggggccc ctcgagagcg tgctcgggat gattccgggg
      901 ctcggcagcc tgaagcagct tggagagaac aagccggacg aaaagcagct tgcgcggatc
      961 gaggcgatca tctgctcgat gacgccggct gaacggagca acgaaaacct gataggcggg
     1021 agccgccgca ggcggatcgc cctcggcagc ggcacgaccg ttgccgacgt gaaccgactt
     1081 ctgaagcagt tctccgacat gaagcggatg ctgcagatga tcggccgcgg cggcacggcg
     1141 gccctgaagg ggatgaaagg gatgaaaggg atgccgaagc tgtcgcatca ggcgcaggcc
     1201 ggcttcgcac aggcgggcgg caggaagcgg aagaagggcg gaccgtgggg gttgatcaag
     1261 tcgcggtgac cgtagaccga agaccgtaga ccggagtaac agatgctagc cattcgaatg
     1321 agacgtgtag gcgcgaagaa gaagccctac ttccgtgtgg ttgtcaccga ggcgaagtcg
     1381 gcgcgcgaga gtaccttcgt cgagaacgtg ggaacgtacc atcccagcga gaaaccggcg
     1441 cgcgtggaga tcaacaaggc acgcgtgcag cactggttgc agcttggcgc tcaaccgtcg
     1501 aactcggtgc gcacgctgct cgccaggcac ctgtcgcgcg acctgtcggc cccgaccgag
     1561 gcgccggctc agtgacgacg atggaagaag aagaaggcca gagcgctgcc aaggccagtc
     1621 ccatccgtgc cgtcgtcgag gtcgtggcga aggcgctggt cgatcgtcct gaacaggtca
     1681 aagtgaccga atcggtccga cggggcatga cggtggtgga gttgacgacg gcgcccaacg
     1741 atatggggaa gatcatcggc cggcaggggc gaacggccgc cgccctgcgg acggtcgttg
     1801 cgatggcggc ggagcgtctc ggggtccgcg cgcaactgga tatacgggac tgaatcaact
     1861 cccaactccc aatgcccaac tcccaagcct gatcgcgctg gaagttagga accgggcttt
     1921 tgggagttgg aggttggaag ttgggagttg attgggaccg gatggtactc gtcgggcgca
     1981 tcgcgcggcc gcacggcctt cgcggccagg ttgtcgtgac gccagagacc gacttcgccg
     2041 aggaccgatt ccgggcgggc gcgacgctct ggacgaggtc gaggcgcggg gacgaggtgc
     2101 tgacgatcag cacggcgcgc ctgcagaatg ggcggcccgt catcgggctc gaaggcttcg
     2161 cgaccatcga ggaggtcgag cggctggcgg gtctcgagct cagggtgccc gaagaggcgc
     2221 tgaagccgct cgccgatggc gcgtactacg tgcaccagct cgtgggctgc gtcgtggaaa
     2281 ccgtcgccgg ccaacgcgtg ggtgaagtgg cgcgcgtgga g
//
LOCUS       DBNGSH010000249         5215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2736070, whole genome shotgun sequence.
ACCESSION   DBNGSH010000249 DBNGSH010000000
VERSION     DBNGSH010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2736070"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="AC16CY_05720"
     CDS             <1..1442
                     /locus_tag="AC16CY_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3353314.1"
                     /translation="LAQSAVQPTVIRLMNELRPDLVGLSVMTFQRGTARRIISLVKAL
                     QPSVRIVVGGYDPSLAPEAWIDPSLGIDFIVRGEGDITFRELLRALDRNAPLSGIDGL
                     WYRTADGFQRNPPRPAARLEDGEIRPPARGGRVLSGYTMMGRPIEVIETSRGCTFDCS
                     FCSIIEMRGRNFHRFPIGRVIDDIRDARRRGATSVFLVDDNVTIDVPRLEALCRAIID
                     HGLDDVQYVVQAMTAPIATHGATLAPLMQRAGFRYVFLGIENVLEGDLAFLKATAKNS
                     RRRTGQVQNTTHAAIDVLHRHGMLVVGGLIVGNPDDTPAAIETNLAFARKYVDWPYIQ
                     HPTPYPGTPMTREFRERGLIVNSRVEEYDGTSAVTRSAHLEAEEIEFMRWKAERWMKV
                     RHMPTAVRLHPGFVLRNAHRMMAHTFRGTTWRSLVGLETAKDVFRRYRQIRARERHYL
                     DWPDPLSPADEASHQERSGAPLRWVASGR"
     gene            1635..2597
                     /locus_tag="AC16CY_05725"
     CDS             1635..2597
                     /locus_tag="AC16CY_05725"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR3353315.1"
                     /translation="MGLPKTGKIWMNGTLVDWQDAKIHIASHVVHYGSAVFEGARCYD
                     TLSGPACFRLDAHMRRLLDSARIYRMECLYDQQTLTDATLDLIRVNGFRACYIRPIVY
                     RGYDSLGVDPRPCPIDTAIMLWEWGAYFTKEAIEEGLDVKISTWARNAPNTTPAMAKS
                     VANYANAQLIKMEAIADGYAEGIALDTQGNLSEGSGQNLFVVRDNIIYTPPIGNSVLW
                     GITRDSVITIAHDLGFEVREQTLPRETLYIADEVFFVGTAVEVTPIRSVDRVKVGRGR
                     RGPVTEAIQRRFFQIVKGEAPDTHGWLLPVGPREAATTGASKSR"
     gene            2663..3820
                     /locus_tag="AC16CY_05730"
     CDS             2663..3820
                     /locus_tag="AC16CY_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3353316.1"
                     /translation="MHVNDLLKLAVQSGSSDLHLKVGTYPMIRVRGTLIPATEERRLE
                     HEDVVAMAAAVLPAGLRDKFRDNHEVDLAYSVAGLGRFRCNAFQQRGTIGMVFRVIPM
                     RVASIEELMLPKVLKKIAAEERGLVLVTGTTGSGKSTTLAALIDEINSSRTAHIMTIE
                     DPIEYLHRDNRSVINQREIGVDTRSFGDALRSALRQDPDVILVGEMRDHETIQTALLA
                     AETGHLVFSTLHTLDATETINRIIAVFPPHQQKQVRIQLSSVLKAVISQRLMPKADGK
                     GRVPAVEVLISTAYLRDCILDKEKSHLIHGAIAQGTSQYGMQTFDQSVFWLYEQGLVT
                     YEEALRWASNVDEFKLKVQGIHTTADMARDEMARTGQSGGARAPEITRFGG"
     gene            3842..4387
                     /locus_tag="AC16CY_05735"
     CDS             3842..4387
                     /locus_tag="AC16CY_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353317.1"
                     /translation="MATYRIDRVDVEATGTSRSAWTFAGRMVKGVEHRYDVTDRNSTS
                     RNKWSFVVRVPHDTHGRIEVRPVATPGDRSYAGLDRRSITFMRATRPGYTAFRYCPLA
                     LADPTGDSSRVIARSTDKGHLPPWLRGLGRRLKKKDTVRTTRGTDGGMLVVLVRPDDH
                     QFMIAMFMATKAWVLKRRFTL"
     gene            4402..4863
                     /locus_tag="AC16CY_05740"
     CDS             4402..4863
                     /locus_tag="AC16CY_05740"
                     /inference="COORDINATES: protein motif:HMM:NF047066.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR3353318.1"
                     /translation="MSNDTYVAALKMLAARELSEAQVRQRLARRQHDQDSIDAAIARL
                     KADGGLDDTRAAGAIARSQTTLKKRGKLRVRREIEAAGIAPSIARHAVDEAFAGLDVE
                     ALLSAALARRLRAGARIADDKEFQRLYRYLLTQGFEADHVVAALRKRRRSD"
     gene            4932..>5215
                     /locus_tag="AC16CY_05745"
     CDS             4932..>5215
                     /locus_tag="AC16CY_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR3353319.1"
                     /translation="MTSREIRASFLSYFERHGHRIVASSPLVPHEDPTLLFTNAGMNQ
                     FKDALLGKETRSYKRAATSQKCMRVSGKHNDLDNVGPSLRHHTFFEMLGN"
BASE COUNT          938 a         1789 c         1667 g          821 t
ORIGIN      
        1 tcctcgccca atccgccgtc cagccgaccg tcatcagact gatgaacgag ctccgcccgg
       61 atctggtcgg tctctcggtg atgacgttcc agcgcggcac ggccaggcgc atcatctcgc
      121 tcgtcaaagc ccttcagccc agcgtccgaa tcgtggtcgg aggctatgac ccgtcgctgg
      181 cgccggaggc ctggatcgat ccgtcgctcg gaatcgactt catcgtccgc ggcgagggcg
      241 acatcacctt ccgggaactg ctgcgtgcgc tcgaccggaa cgcgccctta tccgggatcg
      301 acgggctctg gtacaggacc gccgacggct ttcagcggaa tccgccccgc cccgcggcgc
      361 ggctggagga cggcgagatc cggccgccgg cgcgcggagg gcgcgtgctc tccggctaca
      421 cgatgatggg ccggccgatc gaggtcatcg agacctcgcg cgggtgcacg ttcgactgca
      481 gcttctgctc gatcatcgag atgcgcgggc gcaactttca ccgctttccg atcggccggg
      541 tgatcgacga catccgcgac gcgcgacgcc gaggcgcgac ctcggtgttc ctggttgacg
      601 acaacgtcac gatcgacgtg ccgcggctcg aggcgctgtg ccgggccatc atcgaccacg
      661 gactcgacga cgtgcagtac gtggtgcagg ccatgaccgc gccgattgcc acgcacggcg
      721 ccacactcgc ccccctgatg cagcgagccg gcttccgcta cgtgttcctc ggcatcgaga
      781 acgtgctcga aggagacctc gcgttcctca aggcgaccgc caagaacagc cggcgccgga
      841 ctggccaggt ccagaacacg acgcacgccg cgatcgacgt gcttcaccgg cacggcatgc
      901 tcgtcgtcgg tggcctcatc gttgggaacc cggacgacac gccggcggcc atcgaaacca
      961 acctggcgtt tgcgcgaaag tacgtggact ggccgtacat ccagcacccc acgccgtacc
     1021 ccgggacgcc gatgacgcgg gaattccgcg agcgcggcct catcgtcaat tcgcgagtcg
     1081 aggagtacga cggcacatcg gccgtgacgc ggagcgcgca cctggaggcg gaagagatcg
     1141 agttcatgcg ctggaaggcc gagcgctgga tgaaggtccg gcacatgccg accgctgttc
     1201 gccttcatcc cggcttcgtg ctgcggaacg cccaccgcat gatggcgcac accttccggg
     1261 gaacgacctg gcgatcgctg gtgggtctcg aaacggcgaa ggacgtgttc cggcggtacc
     1321 ggcagatccg ggcccgcgaa cggcactacc tggattggcc cgatccgctc tccccggccg
     1381 acgaggcatc gcaccaggaa cgatccggcg cgccgctccg ctgggttgcc agcggccgtt
     1441 agcaccgcga acgaccgact gccccctgcc ccctgacccc ttcgcaatgt ccccgtgaca
     1501 ttgggtaatt ccaggaaagc cgggttacga tctaagcaac tcccaattcc caattcccaa
     1561 ctcccaaggc gggcgactct cagattgggc gttgggagtc ggatgttggg agttcgcgat
     1621 cgaagggagc acgtatgggc cttccgaaaa ccggcaagat ctggatgaac ggcacgctcg
     1681 tggactggca ggacgccaag atccacattg cctcgcatgt cgttcactac gggagcgcgg
     1741 tgttcgaggg agcgcggtgt tacgacacgc tcagcggccc ggcctgcttc cgcctcgacg
     1801 cccacatgcg ccggctgctc gattcggccc gcatttaccg catggagtgt ctctacgatc
     1861 agcagacgct caccgatgcc acgctcgacc tgattcgtgt gaacgggttt cgggcctgct
     1921 acatccgtcc catcgtctat cgcggctacg actcgcttgg cgtcgacccg cgcccctgcc
     1981 ctatcgacac ggcgatcatg ctctgggagt ggggcgccta cttcacgaag gaggcgatcg
     2041 aggaaggcct cgacgtcaag atctcgacct gggcgcgtaa tgccccgaac acgacgcctg
     2101 ccatggcgaa gagcgtggcc aactacgcca acgcgcagct catcaagatg gaggcgatcg
     2161 ccgacggcta cgccgaaggc atcgcgctcg acacgcaagg caatctcagc gagggcagcg
     2221 gccagaacct cttcgtcgtg cgcgacaaca tcatctacac cccgcccatc ggcaactcgg
     2281 tgctctgggg catcacgcgc gactcggtga tcaccatcgc gcacgacctc ggcttcgagg
     2341 tccgcgagca gacccttccc cgcgaaacgc tctatatcgc ggacgaggtg ttcttcgtgg
     2401 ggacggcggt ggaggtcacg cccatccggt cggtggaccg ggtcaaggtc gggcgcggcc
     2461 ggcggggccc cgtcaccgag gccatccagc gccggttctt ccagatcgtc aagggcgagg
     2521 ctcccgacac ccacggatgg ctgctgccgg tcggtccgag ggaagccgct actacggggg
     2581 cctcgaagag ccgataatcc cggcggcagg tcccgacggt ccggcgcgct gcgcccattc
     2641 ccgtcgggcc tgaccccgcg acatgcacgt caacgatctt ctgaaactgg cggtccagag
     2701 cgggtcgtcc gacctccatc tgaaggtcgg gacctacccg atgatccgcg tccgcggcac
     2761 gctcattccg gccaccgagg agcgccgcct cgagcacgaa gacgtcgtgg cgatggcggc
     2821 cgccgtgctg cccgccggcc ttcgggacaa gtttagggac aaccacgagg tggacctcgc
     2881 ttacagcgtg gccggactgg gccggttccg ctgcaacgcg ttccagcagc gcggcaccat
     2941 cgggatggtc ttccgcgtga ttccgatgcg ggtggcgtcg atcgaagagc tgatgctgcc
     3001 gaaggtcctg aagaagatcg cggcggagga acggggcctg gtgctcgtca cggggacgac
     3061 gggcagcggc aagagcacga ccctggcggc gctcatcgac gagatcaact cgtcgcgcac
     3121 cgcgcacatc atgacgatcg aggacccgat cgaatacctg caccgcgaca accggtccgt
     3181 gatcaaccag cgtgagatcg gcgtggacac tcgatcgttc ggcgacgcgc tgcggagcgc
     3241 gctgcggcag gacccggacg tcatcctggt cggcgagatg cgcgaccacg aaacgatcca
     3301 gaccgcattg ctcgcggcgg aaaccggcca cctcgtattc tcgacgctcc acacgctcga
     3361 tgcgaccgag acgatcaacc gcatcatcgc cgtgttcccc ccgcaccagc agaagcaggt
     3421 ccgcatccag ctgtccagcg tcctcaaggc cgtcatctcg cagcgcctga tgccgaaggc
     3481 cgacggcaag gggcgcgtgc cggccgtcga ggtcctgatc tcgaccgcgt atctccggga
     3541 ctgcatcctc gacaaggaga agtcgcacct gattcacggc gctatcgcgc agggcacctc
     3601 gcagtacggc atgcagacgt tcgaccagtc ggtgttctgg ctgtacgagc agggcctcgt
     3661 cacctacgag gaggcgctgc gctgggcgtc caacgttgac gagttcaagc tcaaggtgca
     3721 gggcatccac acgacggccg acatggcgcg ggacgaaatg gcccgcaccg ggcagtcggg
     3781 cggcgcgcgg gcaccggaga tcactcgttt cggcggctaa ctcgactctc ggctcctggc
     3841 tatggcgacg tacagaatcg accgggtcga cgttgaagcg acggggacct cccgatccgc
     3901 atggaccttt gccggccgga tggtcaaggg ggttgaacat cggtacgacg tgaccgacag
     3961 aaactccacg tcgcggaaca aatggagctt cgtcgtacgc gttcctcacg acacgcacgg
     4021 ccgcatcgag gtgagaccgg tggcgacgcc tggcgacagg agctacgcag gcctcgatcg
     4081 ccggtcgatc acgttcatgc gcgcaacgcg cccgggctac acggccttcc gatactgccc
     4141 gttggcgctc gcggatccaa cgggcgactc gtctcgcgtc atcgcccgct cgacggacaa
     4201 gggtcatctg ccgccctggc tacgcgggct gggccggcga ctgaagaaga aggacaccgt
     4261 cagaacgacg cgcggcaccg atggaggcat gctggtcgtc ctcgtccgcc ccgacgatca
     4321 ccagttcatg atcgccatgt tcatggcgac gaaggcctgg gtcctgaagc gccgtttcac
     4381 gctctgacgc gagcgaccct cgtgtccaac gacacgtacg ttgccgctct gaagatgctc
     4441 gcagcgcgtg agctctccga agcgcaggtt cgtcagcgcc tggcccggcg ccagcacgat
     4501 caggactcaa tcgacgccgc catcgcgcgg ctcaaggccg atggcggtct ggatgacacg
     4561 cgggcggcag gtgcaatcgc ccggtcgcag acgacgctca aaaagcgcgg caagctcaga
     4621 gtcaggcggg aaatcgaggc agcaggcatc gccccttcca tcgcccggca tgccgtggac
     4681 gaagccttcg ccggcctcga cgtcgaggcg ctgctgtcgg cagcgctcgc ccggcggctg
     4741 cgcgccggcg cccgtatcgc cgacgacaag gagtttcaac gcctgtatcg ctacctgctg
     4801 acgcaggggt tcgaagccga tcacgtcgtc gcggccctgc gcaagcgacg ccggtccgac
     4861 tgacgccaga cgctgcgctg cgtacgggca gacgtagtgt ccggctttag ccggacctac
     4921 aatagtcagc aatgacctct cgggagattc gcgcctcctt cctctcctac ttcgaacggc
     4981 acggccaccg catcgtcgcc agctcgccgc tcgtgccgca cgaggatccg acgctgctct
     5041 tcaccaacgc cgggatgaac cagttcaagg acgcccttct cggcaaggag acgcgcagct
     5101 acaagcgggc ggcgacctcc cagaagtgca tgcgcgtcag cggcaagcac aacgacctcg
     5161 acaacgtcgg gccctcgctc cggcatcaca cgttcttcga gatgctcggc aactt
//
LOCUS       DBNGSH010000250         8196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3755228, whole genome shotgun sequence.
ACCESSION   DBNGSH010000250 DBNGSH010000000
VERSION     DBNGSH010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3755228"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="AC16CY_05750"
     CDS             complement(<1..1638)
                     /locus_tag="AC16CY_05750"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR3353320.1"
                     /translation="MGSAALSEAVKSIREWSMGEGRATSAPVKAAELFGSLVFSDKAQ
                     QERLPKAAYRALRATITRGEPLDASTADAVAAALKDWAVEHGATHYTHWFQPMTGITA
                     EKHDSFLAPTTEGTAIAEFRGKELIKGEPDASSFPSGGMRSTFEARGYTAWDPTSPPW
                     LLFNGGNSVTLVIPTAFVSWTGEALDKKTPLLRSMEAVSKQAVRVLKLFGSNAERVYA
                     TCGPEQEYFLIDRNFYFARPDLINAGRTLFGAKPPKGQEMEDQYFGAIPERVLACMAE
                     VELELYKVGVPVKTRHNEVAPSQYEIAPVFENANLAVDHQMMTMEMLKKIAPKYGLAC
                     LLHEKPFAGVNGSGKHNNWSLSDEFGNNLLGPGDTPHDNLQFLVFCAAVLRAVDRWQG
                     LLRASIASAGNDHRLGANEAPPAILSIFLGEMLTDIFEQIEKGGGAKSTKQGGIQDLG
                     VSVLPKLPRDAGDRNRTSPFAFTGNKFEFRAVSSGQSIAFPNMVLNAAMADSLDSIAN
                     ELEAATAKGEELNKAVGKLLTKIIKEHKRIIFNGNNYS"
     gene            1959..3377
                     /gene="glnA"
                     /locus_tag="AC16CY_05755"
     CDS             1959..3377
                     /gene="glnA"
                     /locus_tag="AC16CY_05755"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3353321.1"
                     /translation="MTPDDFFAFAKKHEAEMVDLKFVDMLGSWQHCSFPVEHLDKNTF
                     KDGLGFDGSSIRGWMGIHESDMLAVPDADTARLDPFFARPTVSVIANIVDPVSGKEFS
                     RDPRYVARKAEAHLKETGVADTCFIGPEPEFFIFDEVRYEQNQHRGYYQIDSVEGAWN
                     TARIEEPNLGYKPSFKGGYFPVSPTDTYHDLRGEMVYEMRKVGIVVEAHHHEVATAGQ
                     SEIDMQFQPLLKMGDQFMWYKYICKNVAKRHGKTVTFMPKPIFEDNGSGMHSHTSLWK
                     GGKPLFAGDGYAGLSQIGLFAAGGLLKHAPAILAFAAPTTNSYRRLVPGFEAPVNLAL
                     SARNRSASIRIPMYSKSPKAKRLEFRCPDPSCNGYLAWSAMLMAMLDGIQNKIDPGQP
                     LDRDIYEMTRDEMKEMGIKTTPGSLEEALDALEQDHAFLTKGDVFTDDLIDAWISYKR
                     DKELDPIRLRPHPHEFYLYYDN"
     gene            3481..7203
                     /gene="metH"
                     /locus_tag="AC16CY_05760"
     CDS             3481..7203
                     /gene="metH"
                     /locus_tag="AC16CY_05760"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018218386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR3353322.1"
                     /translation="MTLDEILARRIAILDGAMGTMVQRERLDEAAVRGARFRDHGHSL
                     RNNIDLLVLTRPDLISAIHHAYLEAGADIIETNTFSSTSVAQADFGLSSAVHELNVAA
                     ARLARDAADAWTARTPERPRFVAGAIGPTNRTLSISPDVGNPALRAETFSGLRRAYRE
                     QARALVEGGVDLLLVETVFDTLNAKAAIAAILDEFDARGAALPVMISATITDRSGRTL
                     SGQTLDAFYISIQHLRPFSVGLNCAFGARQMRPYMAELSRVAGEYVSCHPNAGLPNAF
                     GEYDEPPEETARLLKDFAASGYVNIIGGCCGTTPEHIRAIRHAVDGMPARQTGLRAQG
                     PGVSEPQTPEGFTQYSGLESLTIRPDSSLIVIGERTNVAGSKRFAEMIADGRYGDAAS
                     VALEQVRGGANILDINMDEGMLDSETAMTTFLNVVATEPEIARLPFMIDSSKWSVLEA
                     GLQCVQGKAIVNSISLKDGQDEFLRRAATIRRYGAALVVMAFDERGQADTAGRKIEIC
                     ERAYRLLVDRLACDPRDIVFDLNVLAIATGLDEHAFYAMDFIDAARVLKATCPGVKVC
                     GGVSNLSFAFRGNARVREAMHSAFLYHAVRAGLDMAIVNAGQLTVYETIPKDLLALVE
                     DVIFNRRPDATDRLVQFARTVTGPASSRSADLAWRDANVHQRLEHALVHGIVDYIDDD
                     VGEALRDLRRPLEVIEGPLMNGMRTVGELFGSGQMFLPQVVKSARVMKKAVAYLLPYM
                     EEETGGKGEGGQGEEGQGAGGRGRQRPKIVLATVKGDVHDIGKNIVGVVLGCNNYEVI
                     DLGVMVPADRILQTAVDEQADLIGLSGLITPSLDEMVFVAREMTRRGMTTPLLIGGAT
                     TSRQHTAVKIAPEFTDRPVVHVVDASRAVDVVSRLLDPSRRDEFSAANRQDQDRSREQ
                     HAALKRRPLVAWPDASKNRLRLEWTVPATPSFTGTRVIDVDLREVTPYIDWTFFFTAW
                     ELRGRFPAILDHPQYGPAARDLYDNARVLLDRIVSESLLRAKAVYGFWRANADGDDIV
                     LYDSDRKVERFPMLRQQEQMPAGTPHLSLADFVAPRESGIADSVGAFAATAGIGVDAL
                     VSAYERDHDDYHAIMIKALADRLAEASTEWLHARARAEWGFGLSEGLSPADVLAERFR
                     GIRPAFGYPACPDHSEKQRLFHLLRAERAGLSLTESFAMAPAASVSGLYFAHPSARYF
                     SVGRIGRDQVEDYASRKGASVADVERWLRPNLAYEP"
     gene            7315..7908
                     /locus_tag="AC16CY_05765"
     CDS             7315..7908
                     /locus_tag="AC16CY_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353323.1"
                     /translation="MEYLIKQYPLETGALEIQYIEEYFGEFPRKKTAAEIVARLRDRE
                     FLILMAEAPLPDDPGTVVPVAFKIVHEIRIRETEPKLVDLVARLHGCVQFQGAKVLYS
                     WIGGTRRDWRSQGHFRALTEESEAWAHSAGFHEVVVKTKNRYYDMRGTLDQLEFNVIK
                     YEVDRDDNRESKVYLSKRLTSDVLRSHTSSRRVVVAD"
     gene            complement(7957..>8196)
                     /locus_tag="AC16CY_05770"
     CDS             complement(7957..>8196)
                     /locus_tag="AC16CY_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3353324.1"
                     /translation="DLVSELPSPEEEAVREEAYDHYQACLSQLSARDRELIVSRVEVG
                     WSMDQIARKFGMRTTDAARMAVTRALRRLAGLKKS"
BASE COUNT         1434 a         2750 c         2670 g         1342 t
ORIGIN      
        1 cgagtagttg ttgccgttga agatgatccg cttgtgctcc ttgatgatct tcgtcagcag
       61 cttgccgacc gccttgttca gctcttcgcc cttggcggtt gccgcttcca gctcgttcgc
      121 gatcgagtcg agcgaatcgg ccatggccgc gttcagcacc atgttcggga aggcaatgct
      181 ctggcccgag gacaccgcac ggaactcgaa cttgttgccg gtgaacgcga atgggctggt
      241 gcggttgcgg tcgccggcgt cacgcggcag tttcggcagg acgctgacgc cgaggtcctg
      301 aatcccaccc tgcttggtgg acttggcgcc gccgcccttc tcgatctgct cgaaaatgtc
      361 ggtgagcatc tcgccgagga agatcgacag gatcgccggc ggcgcttcgt ttgccccgag
      421 ccgatgatcg ttgcccgcgc tggcgatgct ggcgcgcagc aggccctgcc accgatcgac
      481 ggcgcgaagc acggccgcgc agaagaccag gaactgcagg ttgtcgtgcg gcgtgtcgcc
      541 ggggccgagc aggttgttgc cgaactcgtc ggagagcgac cagttattgt gcttgcccga
      601 tccgttgacg ccggcaaacg gtttctcgtg gagcaggcag gccaggccgt acttcggcgc
      661 gatcttcttc aacatctcca tcgtcatcat ctggtgatcg acggcgaggt tggcgttctc
      721 gaagaccggc gcgatctcgt actggctcgg cgcgacctcg ttgtgccgcg tcttcacggg
      781 gacgccgacc ttgtacagct cgagctcgac ttcggccatg cacgcgagca cgcgctcggg
      841 gatggcgccg aagtactgat cttccatctc ctgccccttc ggcggcttgg cgccgaagag
      901 cgtgcggccc gcgttgatca ggtcgggccg cgcaaagtag aagttgcggt cgatgaggaa
      961 gtactcctgc tcgggtccgc acgtcgcgta gacgcgctcg gcgttcgacc cgaagagttt
     1021 gaggacgcgc accgcctgct tcgacaccgc ttccatggag cgcagcagcg gcgtcttctt
     1081 gtcgagcgcc tcgccggtcc agctcacgaa cgcggtcggg atgacgagcg tcacgctgtt
     1141 gccgccgttg aacagcagcc agggcgggct ggtgggatcc cacgccgtgt agccgcgcgc
     1201 ttcgaacgtg gaccgcatgc cgccggacgg gaagctcgac gcatccggct cgcccttgat
     1261 caattccttg ccgcggaact cggcgatcgc ggtgccttcc gtggtcgggg cgagaaacga
     1321 gtcgtgcttc tcggcggtga tgcccgtcat cggctggaac cagtgcgtgt aatgcgtcgc
     1381 gccgtgctcg accgcccagt ccttcagggc cgccgcgacg gcatcggccg ttgacgcgtc
     1441 gagcggctcg cctcgcgtga tcgtcgctcg aagcgcgcgg tacgccgcct tcggcaggcg
     1501 ctcctgctgg gccttgtcgc tgaacacgag gctgccgaac aactcggcgg ccttgactgg
     1561 cgcagacgtt gcgcgcccct cccccatcga ccactcacgg atgctcttga cggcttccga
     1621 cagcgctgca gatcccatgt ggcgatcaat cctttcttcg atcggatgtg tgcgatgaat
     1681 tgtgtgggtg acgcccgtac cacccgtggc tcgggcgacg cggaccgagc gggacatccg
     1741 attatgtcgc gtccgaggcc tgccctgcaa tacggttggg ccggagacgc gcccatcggg
     1801 ccggcgccgg ccccctccaa agcaacaaaa atgaaacgcc ggacccggtt tccgccaaga
     1861 tccgctatgc tttcgaccca catttccttt cgcccgcccc actccacatg ccactcgccg
     1921 tcacgggcgg gggctgaacc gacgctggag gtgactcgat gacgcccgac gacttcttcg
     1981 cctttgccaa gaagcacgag gccgagatgg tggacctgaa gttcgtggac atgctcggca
     2041 gctggcagca ttgctcgttc cccgtggagc acctggacaa gaacacgttc aaggacggcc
     2101 tcgggttcga tggatcctcg atccggggct ggatggggat tcacgaatcc gacatgctcg
     2161 ccgtgcccga cgccgacacg gcgcgcctcg acccgttctt cgccagaccg accgtcagcg
     2221 tcatcgccaa catcgtcgac ccggtctcgg gaaaggagtt ctcccgcgat ccgcgctacg
     2281 tcgcgcgaaa ggccgaggcg cacctgaagg agacgggcgt cgccgacacc tgcttcatcg
     2341 gccccgagcc cgagttcttc atcttcgacg aggtccgcta cgagcagaac cagcatcgcg
     2401 gctactacca gatcgactcg gtcgaaggcg cgtggaacac cgcccggatc gaggagccga
     2461 acctggggta caagccgagc ttcaaggggg gctacttccc ggtgagcccg accgacacgt
     2521 accacgacct gcgcggcgag atggtgtacg agatgcgcaa ggtcgggatc gtcgtggaag
     2581 cgcaccatca cgaagtcgcc acggccgggc agagcgagat cgacatgcag ttccagccgc
     2641 tgctgaagat gggcgaccag ttcatgtggt acaagtacat ctgcaagaac gtcgcgaagc
     2701 gccacggcaa gacggtgacg ttcatgccca agccgatctt cgaggacaac ggcagcggca
     2761 tgcactcgca cacctcgctg tggaagggag gcaagccgct ctttgcgggc gacggctacg
     2821 ccggcctcag ccagatcggg ctcttcgcgg cgggaggact gctcaagcac gcgccggcga
     2881 ttctcgcctt cgcggccccc acgaccaatt cctaccggcg gctggtgccc ggattcgagg
     2941 cgccggtcaa cctcgcgctc tcggcccgaa atcgttcggc gtcgatccgc atcccgatgt
     3001 actcgaagag cccgaaggcc aagcggctgg agttccgctg tcccgacccg agctgcaacg
     3061 gatacctggc gtggtcggcg atgctgatgg ccatgctcga cggcatccag aacaagatcg
     3121 atcccggcca gccgctcgac cgcgacatct acgagatgac gcgggacgag atgaaggaga
     3181 tggggatcaa gacgacaccg ggctcgctcg aagaagcgct cgacgcgctg gaacaggacc
     3241 acgccttcct gaccaagggc gacgtattca ccgacgatct gatcgacgcc tggatttcgt
     3301 acaagaggga caaggagctg gatccgatcc ggctgcggcc gcatccgcac gagttctacc
     3361 tgtattacga caactgatcg ctctcacgaa ccacggagtc acagaggcac ggagaaccac
     3421 gaccaattct ctgtgtctcc gtgtctccgt ggttcacgct ccgtggttta agcttctcgg
     3481 gtgaccctcg acgagatcct cgcccgccgc atcgcgatcc tcgacggcgc gatgggtacg
     3541 atggtgcagc gggagcgcct cgacgaagcc gccgtccgcg gcgcacggtt ccgcgatcac
     3601 ggccactccc tccggaacaa catcgacctg ctcgtcctca cgcgcccgga tctgatctcg
     3661 gcgatccatc acgcctatct cgaggccggc gccgacatca tcgaaaccaa cacgttcagc
     3721 agcacgtcgg tcgcgcaggc agacttcggg ctgagctccg ccgtgcacga gctgaacgtc
     3781 gcggcggccc gcctggcgcg cgacgcggcg gacgcctgga ccgcgcggac gccggaacgg
     3841 cctcgcttcg tcgcgggcgc catcgggccg accaaccgca cgctgtcgat ctcgccggat
     3901 gtgggcaacc cggcgctccg tgccgagacc ttcagcggtc ttcgccgtgc ctatcgggag
     3961 caggcgcgcg cgctcgtcga gggaggcgtg gacctgctgc tcgtcgagac cgtgttcgac
     4021 acgctgaacg cgaaagcggc gatcgcggcg attctcgacg agttcgacgc gagaggcgcg
     4081 gcactgccgg tgatgatctc cgccacgatc acagaccgca gcggcagaac gctgtcgggg
     4141 cagacgctgg acgccttcta catctcgatt caacacctcc ggcccttcag cgtcgggttg
     4201 aactgcgcct tcggcgcccg gcagatgcga ccgtacatgg ccgaactgtc gagagtggcc
     4261 ggtgagtacg tcagctgcca tccgaacgcc ggtcttccga acgcgttcgg cgagtacgac
     4321 gagcctcccg aagagaccgc ccgcctgctg aaggacttcg ccgcctccgg ctacgtcaac
     4381 atcatcggcg gatgctgcgg aacgacgccc gagcacatca gggccatacg gcacgccgta
     4441 gacgggatgc cggcacgaca gacaggcctc agggcccagg gcccaggcgt cagcgaaccc
     4501 cagactccgg agggcttcac tcaatactcc ggcctcgagt cgctcaccat tcggcccgac
     4561 tcgagcctca tcgtcatcgg cgagcggacc aacgtcgccg gctcgaagcg cttcgccgaa
     4621 atgatcgccg acgggcggta cggcgatgcc gcgtccgtgg cgctcgaaca ggttcgcggc
     4681 ggcgccaaca tcctcgacat caacatggac gagggcatgc tcgattcgga gaccgccatg
     4741 acgacattcc tgaacgtcgt ggcgaccgag cccgagatcg cgcggctgcc gttcatgatc
     4801 gacagctcga agtggtccgt gctggaggcc ggcctccagt gcgtgcaggg caaagcgatc
     4861 gtcaactcga tcagcctcaa ggacgggcag gacgaattcc ttcgacgagc cgccacgatc
     4921 cgccggtacg gcgccgcgct cgtggtgatg gcgttcgacg agcgaggcca ggccgatact
     4981 gccggccgga agatcgagat ctgcgaacgc gcctaccggc tgctcgtcga tcggctggcc
     5041 tgcgacccgc gtgacatcgt gttcgacctg aacgtcctgg ccatcgccac cggcctcgac
     5101 gagcacgcct tctacgcgat ggacttcatc gacgccgcgc gcgtgctcaa ggccacgtgc
     5161 ccgggcgtca aggtgtgcgg cggcgtcagc aacctctcgt tcgcgttccg cggtaacgcg
     5221 cgcgtgcgcg aggcgatgca ctcggcgttt ctctaccacg ccgtccgggc cggcctcgac
     5281 atggcgatcg tcaacgccgg ccagctcact gtctacgaga cgatcccgaa ggacctcctc
     5341 gcgctcgtcg aggacgtcat cttcaaccgc cggccggacg ccacggatcg tttggtgcaa
     5401 ttcgcgcgaa ccgtcacggg gccggcctcg agccgatcgg ccgaccttgc ctggcgtgac
     5461 gcgaacgtcc atcagcgcct cgagcacgcg ctcgtccacg gcatcgttga ctacatcgac
     5521 gatgacgtcg gcgaggcgct gcgcgatctg cggcgtccgc tcgaggtcat cgaagggccg
     5581 ctcatgaacg gcatgcgcac cgtgggcgag ctgttcggct cggggcagat gttcctgccg
     5641 caggtcgtca agagcgcacg cgtgatgaag aaggcggtcg cgtatctcct cccgtacatg
     5701 gaggaggaga cggggggaaa gggggaaggg gggcaggggg aagaggggca gggggcaggg
     5761 ggccgggggc ggcaacggcc gaagatcgtg ctcgcgaccg tcaaaggcga cgtgcacgac
     5821 atcggcaaga acatcgtggg cgtcgtgctc ggctgcaaca actacgaggt catcgacctc
     5881 ggcgtcatgg tgccggccga ccgcatcctg cagacggccg tggacgaaca ggcggacctc
     5941 atcggcctga gcggcctcat cacgccgtcg ctcgacgaga tggtgttcgt agcccgtgag
     6001 atgacacgcc gcgggatgac gactcccctg ctcatcggcg gcgcgacgac gagccggcag
     6061 cacacggcgg tgaagatcgc gcccgagttc acggaccggc ccgtcgtcca cgtcgtggac
     6121 gcctcgcgcg cagttgacgt ggtgtcgcga ctgctcgatc cgtcgcggcg cgacgagttc
     6181 agcgcagcga accggcagga tcaggatcgg agccgcgaac agcatgccgc gctcaaacgt
     6241 cggcccctcg tcgcgtggcc cgacgcgtcg aagaaccgcc tccggctcga gtggaccgtg
     6301 ccggcgacac catccttcac cggcacgcgc gtcatcgacg tcgacctccg cgaggtcacg
     6361 ccgtacatcg actggacgtt cttcttcacg gcgtgggagc tcagaggccg cttcccggca
     6421 atcctcgacc accctcaata cggacctgcg gcacgggatc tctacgacaa cgcgcgcgtc
     6481 cttctggatc gaatcgtgtc cgaaagtctc ctgcgcgcga aggccgtgta cggcttctgg
     6541 cgcgcgaacg ccgacggcga cgacatcgtc ctgtacgact cggatcgcaa ggtcgaacgc
     6601 ttcccgatgc tccggcagca ggagcagatg ccggccggta ctccccacct gtcgctcgcc
     6661 gacttcgtcg caccacggga gagcggaatc gccgattcgg tgggcgcctt cgccgccacc
     6721 gccggcatcg gcgtggacgc gctggtctcg gcgtacgagc gcgatcacga cgactaccac
     6781 gcgatcatga tcaaagcgct cgccgaccgg ctggcagaag cctccaccga gtggctgcac
     6841 gcgcgggcga gggcggagtg gggcttcggc ctgagcgagg gtctgtcgcc ggccgatgtg
     6901 ctcgccgagc gcttcagggg aatcaggccc gcgttcgggt accccgcctg ccccgaccac
     6961 agcgagaagc agcggctgtt ccatctgctg agggcagagc gggccggcct gtcgctgacc
     7021 gagagcttcg cgatggcgcc ggcggcgtcg gtgagcgggc tctacttcgc gcacccctcc
     7081 gccagatact tttcagtggg ccgcatcgga cgcgatcagg tcgaggatta cgcgagccgc
     7141 aagggggcca gcgtggccga cgtcgagaga tggctgcggc cgaatctggc gtatgaaccg
     7201 tgatgggctc ctggctctcg actctcgccg ctcggctgtc ccagtaccta tagaatgccc
     7261 acaaccagaa ccgagaacag cggcccagag ccaagagccc agagccggga gtccatggag
     7321 tatctaatca agcaataccc gctcgaaaca ggtgcgctcg agatccagta catcgaggaa
     7381 tacttcggcg agttcccgcg gaaaaagacc gcggccgaaa tcgtggcgcg gctccgcgac
     7441 cgcgaattcc tcatcctcat ggccgaagcg ccgctgccgg acgaccccgg gaccgtcgtt
     7501 ccggtggcct tcaagatcgt gcacgagatc cggatccgcg agaccgagcc caagctggtc
     7561 gatctcgtgg cgcggctcca cggctgcgtg cagttccagg gcgcaaaggt gctttacagc
     7621 tggatcggcg gaacgcggcg cgactggcgc agtcagggcc acttcagggc gctgaccgag
     7681 gagtcggagg cctgggcgca ttccgccggg tttcacgagg tcgtcgtcaa gacaaagaac
     7741 cgctactacg acatgcgcgg gacgctcgac cagctcgagt tcaatgtcat caagtacgag
     7801 gtcgatcgcg acgacaaccg tgagtcgaag gtctatctga gcaagcgcct gacctccgac
     7861 gtgctgcgtt cccacaccag cagccggaga gtcgtcgtcg cggactgacg cttgccccgc
     7921 cgcagccgcg gggcggcgaa ggcgggccct ggccggtcag ctcttcttca gcccggccag
     7981 ccggcgcaac gcccgcgtca cagccatacg cgcagcgtcc gtagtccgca tcccgaactt
     8041 ccgcgcgatc tggtccatgc tccacccgac ctcgacgcgg ctcacgatga gctcgcggtc
     8101 gcgggcgctg agctgcgaca ggcaggcctg gtagtgatcg taggcctcct cgcgcacggc
     8161 ttcttcctct ggtgacggaa gctcggagac gaggtc
//
LOCUS       DBNGSH010000251         1669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2566501, whole genome shotgun sequence.
ACCESSION   DBNGSH010000251 DBNGSH010000000
VERSION     DBNGSH010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2566501"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="AC16CY_05775"
     CDS             <1..685
                     /locus_tag="AC16CY_05775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3353325.1"
                     /translation="EHISRVRERYQLDHQFVLYVGNIKPHKNLVRLIEAFSRLRQDHG
                     DIKLLIIGDEISKLPALRRAVHSHKLRKHVRFLGYLKDDTLTVLYRLAAVFVFPSLYE
                     GFGLPPLEAMASGTPVVTSNLSSLPEVTGDAAVLVDPYDVDSIEHGIRRVLDDPSLAD
                     DLRRKGLQRAREFSWERSVEETLRVYRDVAGRGQGVGGRGQGAGGRGQAEKEKESEHG
                     GVAETQRTG"
     gene            682..>1669
                     /locus_tag="AC16CY_05780"
     CDS             682..>1669
                     /locus_tag="AC16CY_05780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3353326.1"
                     /translation="MREGPIRVALVHDWLTGMRGGEKVLEGLCGLHPGADILTLVHRR
                     GSVSQAIERHPIRTSLVQHLPAATTGYRYYLPLFPFAIEQFDLDDYDLVISSSHCAAK
                     GVVPRGDARHICYCHSPMRYAWDQFEAYFGPARVGALASRWFYRPVLSRLARWDVATS
                     VRVNRFVANSAYVAGRIRRYYNREATIVYPPVDTVFYRPADVTPGNHFLIVSALVPYK
                     RIDLAIEACSALGAALRIVGDGPERDRLERQAGAGTTLLGWLPDEALRDEYQRAAAVI
                     MPGEEDFGIVPLEANACGRPVVALARGGATETVEDGDTGVLFTEPTRESLTTA"
BASE COUNT          275 a          542 c          569 g          283 t
ORIGIN      
        1 cgaacacatc tcgcgggtgc gcgagcggta ccagctcgat caccagttcg tgctgtacgt
       61 cggcaatatc aagccgcaca agaacctcgt gcgactcatc gaggcattca gccggctgcg
      121 ccaggaccac ggggacatca agctgctcat catcggcgac gagatctcga agctccccgc
      181 cctgaggcgt gccgtccaca gccacaagct gcgcaaacac gtccgcttcc tcggctacct
      241 gaaggacgac accctgacgg tgctgtaccg gttggcggcc gtgttcgtct ttccctcgct
      301 ctacgaaggt ttcggactgc cgcctctcga ggcaatggcg agcggaacgc cggtcgtcac
      361 ctccaatctc tcctcgctgc ccgaggtgac gggcgacgcg gcggtgctgg tcgaccccta
      421 cgacgtcgat tcgatcgagc acggcatccg ccgcgtgctc gatgacccat cgcttgccga
      481 cgatctgcgg cgcaaggggc tgcagcgggc gcgcgaattc tcgtgggagc ggtccgtcga
      541 agaaacgctg cgcgtgtatc gggatgtagc tggcaggggg cagggggtag ggggcagggg
      601 gcagggggca gggggcaggg ggcaggcgga aaaggaaaaa gagagtgagc acggcggcgt
      661 cgcagagacg cagaggacag gttgagagag ggtccgattc gagtcgcgct cgtgcacgac
      721 tggctgaccg ggatgcgcgg gggcgagaaa gtgctcgagg ggctctgcgg cttgcatccc
      781 ggcgccgaca tcctcacgct cgtccatcgc cgaggctccg tctcgcaggc catcgagcgg
      841 catccgattc ggacctcgct cgtgcagcat ctgccggcgg ccacgacggg ctaccgctac
      901 tacctgccgc tgttcccgtt cgcgatcgag cagttcgacc tcgacgacta cgacctcgtg
      961 atcagctcga gccactgcgc cgcgaagggc gtcgtgccga ggggcgacgc gcggcacatc
     1021 tgctattgcc attcgcccat gcgctatgcc tgggatcagt tcgaggcgta tttcgggccg
     1081 gctcgcgtcg gcgccttggc gagccgatgg ttctaccgcc cagtgctgtc gaggctggct
     1141 cgatgggacg tcgcgacgtc cgtgcgagtc aatcgcttcg tggccaactc agcttacgtt
     1201 gccggccgga tccgtcgata ctataatcgc gaggccacaa tcgtgtatcc gcccgtcgac
     1261 acggtgttct accgtcccgc cgatgtcact cccggcaatc atttcctcat cgtgtccgcg
     1321 ctggttccct acaagcggat cgatctggcg atcgaggcgt gctcggcgct cggcgccgct
     1381 ctgcggatcg tcggcgacgg cccggagcgc gatcgcctcg aacgccaggc cggagcgggc
     1441 acgacgttgc tgggctggct tccggacgag gccctccggg acgagtatca gcgggctgcg
     1501 gcagtcatca tgcccggaga agaagacttc ggcatcgtgc ccctcgaggc gaacgcgtgc
     1561 ggcaggcccg ttgtcgcgct ggcccgcggc ggcgcgacgg aaacggtcga ggacggtgac
     1621 acgggcgtgc tcttcacgga acccactcgc gagtctctga cgacggcgc
//
LOCUS       DBNGSH010000252         1900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1887491, whole genome shotgun sequence.
ACCESSION   DBNGSH010000252 DBNGSH010000000
VERSION     DBNGSH010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1887491"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="AC16CY_05785"
     CDS             complement(<1..819)
                     /locus_tag="AC16CY_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR3353327.1"
                     /translation="MSGRLLVALVASLAVVLAAPFVGQARGALQGALPDHYRNIVLGM
                     VAIGVVAIMAIALTMIRERRLLRYSLLAGAVAGGVVYAYLTRTDNPEVNAVEWFHFVE
                     YGLLTLLFHRAWREAGDLSSLVLPAVAVFVVGTLDEGFQWFVPARVGELRDVFLNLAA
                     IACGLVCGAGIEPPSRLAHVTAHTRRNLSYGLAVAVLALSVFVYTVHMGYHVRDESGD
                     GLLSRFSVEDLRSASIDRGERWKNTPPVELRRLSREDQYLAEGIWHIQQRSIAFR"
     gene            complement(821..1312)
                     /locus_tag="AC16CY_05790"
     CDS             complement(821..1312)
                     /locus_tag="AC16CY_05790"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3353328.1"
                     /translation="MEHVTIVPARPDDVPIILGLIKALADYERLSDQVVATEAGLRVQ
                     LFGDRPAAEVVLAHVDDTVVGFALFFHNFSTFLGQPGLYLEDLFVVPEWRGRGVGRQL
                     LAYLAALAVERRCGRMEWSVLDWNTSAIGFYERLGATLMKDWKLCRLTGESLARAAAG
                     SGR"
     gene            1470..>1900
                     /locus_tag="AC16CY_05795"
     CDS             1470..>1900
                     /locus_tag="AC16CY_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3353329.1"
                     /translation="MAPAVLEAVRKVTVNAATSGPTGFDVFISYSHDDAGLAERLSRR
                     IRRYRPPRAAGPGRRRLRVFRDVERLTAHSSLSEALLQNVRAAKSLVVLCSPSAAASK
                     YVNDEVVAFVECQGTARVHVVLAAGTLQEALPPALSGLADPL"
BASE COUNT          358 a          621 c          611 g          310 t
ORIGIN      
        1 ccgaaacgcg atgctgcgct gctggatgtg ccagatccct tccgcaaggt actggtcttc
       61 tctcgacagc cggcgcagct cgactggagg cgtgttcttc caccgttcgc ctcgatcgat
      121 cgacgcggac ctgaggtctt ccacggagaa gcgggacagg aggccgtcgc cgctttcatc
      181 ccgaacgtgg tagcccatat gaaccgtata gacaaacacc gagagcgcga gcaccgccac
      241 cgccaatccg taagagaggt tgcggcgcgt atgtgcggtg acgtgggcaa gacggctcgg
      301 cggctcgatg ccggcgccgc acacgaggcc gcaggcaatg gccgcgaggt tcaggaagac
      361 gtcccgcaac tcgccgacgc gggcgggaac gaaccactga aacccttcgt cgagcgtgcc
      421 cacgacgaac acggcaacgg ccggaagcac cagcgagctc agatcgccgg cctcacgcca
      481 cgcgcggtgg aacagcagcg tgaggaggcc gtattcgacg aagtgaaacc actccacggc
      541 gttgacctcg ggattgtccg ttcgtgtgag gtaggcgtac accacgccgc ctgccacggc
      601 cccggccaat aggctgtatc gcagaagacg gcgttcccga atcatcgtca acgcgatcgc
      661 catgatggcg acaacgccga tggccaccat gccgaggacg atgttgcggt agtgatccgg
      721 cagggcaccc tgaagcgctc cgcgcgcctg gccaacgaag ggcgccgcca gcaccacggc
      781 cagtgatgcg acgagagcca cgagcagccg gccgctcatg tcaacggccg ctgcccgcgg
      841 cggcgcgcgc gaggctctcg ccggtcaggc ggcacagctt ccagtccttc atcagcgtgg
      901 cgccgagacg ctcgtagaag ccgatcgcgc tcgtgttcca gtcgagcacc gaccattcca
      961 tgcgcccgca ccgtcgttcg accgcgaggg ccgccaggta cgcgagcagc tgtcggccga
     1021 cgccgcggcc tcgccattcg gggacgacga agagatcctc gaggtacagc cccggctggc
     1081 cgaggaaggt cgagaagttg tgaaagaaga gcgcgaagcc gacgaccgtg tcgtcgacgt
     1141 gggcaagcac gacttcggcc gccggacgat caccgaacaa ctggacgcgc aggcccgctt
     1201 cagtcgcgac gacttgatct gagagccgct cgtagtcggc cagcgccttg attaggccaa
     1261 ggatgatcgg gacatcgtcg ggacgcgcgg gcacgatggt gacgtgttcc atggaaactc
     1321 cgacaagcat actgtgctga cacttctcgc ctctgtcttc ccagttcgtc ttccaatttc
     1381 agcctcgttt ctcagcgcgc agttcggggg ttcagggctc ggggtctcgg gttcggaaag
     1441 agttgaccga tggccgattg accgggagaa tggcgcctgc cgtcctcgag gcagtgcgca
     1501 aagtgacagt gaacgcagcg accagtggtc cgacaggctt cgatgtcttc atcagctaca
     1561 gccacgatga cgctggcctc gcggaacgtc tgagccgtcg catccgccga tatcggcctc
     1621 cccgcgcagc tggcccgggc cgacggcgac tcagagtgtt tcgtgacgtc gagcgcctga
     1681 ccgctcatag cagcctatcc gaggcgttgc tgcagaacgt tcgagccgcg aaaagcctcg
     1741 tggtgctttg ctccccaagc gcggccgctt ccaagtacgt aaacgacgag gtcgtcgcct
     1801 tcgtcgagtg ccagggcaca gctcgtgtcc atgtcgttct tgccgctggg actttacagg
     1861 aggctctgcc tccggcgctc agtggactcg ccgatccgct
//
LOCUS       DBNGSH010000253         6558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2566578, whole genome shotgun sequence.
ACCESSION   DBNGSH010000253 DBNGSH010000000
VERSION     DBNGSH010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2566578"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="AC16CY_05800"
     CDS             complement(<1..268)
                     /locus_tag="AC16CY_05800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3353330.1"
                     /translation="MDDRLLDEFRRIVGRDAVLTEPVQLLTYECDALPHLRETPALVV
                     LPGSAADVQAIVRLCSREGLPFVARGHGTGLSGGALPVPGGVVIS"
     gene            335..1609
                     /gene="larA"
                     /locus_tag="AC16CY_05805"
     CDS             335..1609
                     /gene="larA"
                     /locus_tag="AC16CY_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HIR3353331.1"
                     /translation="MRVRLDYGTEGLDVDLPGERTTIIEPMARPAVADARATLLQAIR
                     SPLGRPPLRDLVSAGQKVAISVCDITRAQPRKETIEALFEEMPQVDPKDVTILIATGT
                     HRTNTDQELERMLGREILGRCRVINHNSRERSTLSRVGATSTGVEVYLNREWLGADVR
                     ITTGFVEPHFFAGFSGGPKMVAPGLAGLDTVMTLHDARHIGHPKATWGITEGNPIHDD
                     VREIARMVTVHFAVDVTLNREQKITAAFAGDIFAEHRAACAYAKDTAMRQVPSPFDVV
                     VTTNSGYPLDQNLYQAVKGMSAAAKIVKPGGTIIAAAECRDGLPSHGSYGAVLASAAN
                     PEALLAMICAPGYSVPDQWQVQVQAQIQTKAKVLVKTAGLGPSEIRAAHFTPIDDVSE
                     AVRASLDRAGQGSTLCVLPQGPQTIPYLASEA"
     gene            1620..2459
                     /locus_tag="AC16CY_05810"
     CDS             1620..2459
                     /locus_tag="AC16CY_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR3353332.1"
                     /translation="MSDPKNILLAVLVAVTVVFIAFWSRASWRHLAIGAVTNFFDTLG
                     IGSFATTTTMYRLWRLVKDENIPGTLNVGHALPTVAQALIYITIVEVDFRTLILLILS
                     AVGGSWIGAAIVAGLPRAAIQRGMGVALMVAAVLMVMTALDRFPGGGTALELTAGKLV
                     VACAINFALGALMTLGIGAYAPIMIMVSLLGMNPTAAFPIMMGSCAFLMPIAGYQFIR
                     KSRYDLAASIGLTIGGIPAVLAAALIVRSLPLYWVRWLVVVVVVYAAISLLNSARHEG
                     RKR"
     gene            complement(2514..3350)
                     /locus_tag="AC16CY_05815"
     CDS             complement(2514..3350)
                     /locus_tag="AC16CY_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR3353333.1"
                     /translation="MGLLPRPSIAAVVTCVALLAAAGAVEAQRFFREGSFAPRWAPPQ
                     MPDASFVVCRLAYRSVRTEPSGIGWQTDYPYAEVNLTTRLSELTKARVSLDESRQPNF
                     YVVRFTDDALFNCPIVVASDAGTIGIDEHEGARLRTYLLKGGFLWVDDFWGPEAWDHW
                     ASQITSVLQPPEYRIEDVPLSDPMLRTMYEIKQVPQITNIQFWRRWGGAETSERGPDS
                     AEVHLRVIRDHHRRVMVVMTHNTDIADSWEREGEDPAFFYQFSPAGYALGIDVVIHAM
                     TH"
     gene            complement(3394..4119)
                     /locus_tag="AC16CY_05820"
     CDS             complement(3394..4119)
                     /locus_tag="AC16CY_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353334.1"
                     /translation="MEVRDGFIVGIFNYCDRWCETCAFTSRCRVFADVAEIEARLDPG
                     LKEIVEAPPLPQDQPPPPPAWMQEMIDEMNAAANGAAATAELRPPRPIPPEHRVIEAR
                     ADDYCTRARAWLREHPDRGHDPGDPRAVIAWFHLQIAVKIHRAIRGLAEDDPDEREWP
                     ADHDGSAKVALLGIDRSHAAWLDAVERGVTSDSKAAPLVADLVWLGEALERVFPRARA
                     FVRPGFDEPDEVSSLLAREAGKS"
     gene            complement(4351..4773)
                     /locus_tag="AC16CY_05825"
     CDS             complement(4351..4773)
                     /locus_tag="AC16CY_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3353335.1"
                     /translation="MPRSCLVDSGALVALLDPREAHHTWARETLPRQPRPWLTCESVL
                     SEALYLLRQPQAAALDQLLRQEHLQLALDLAEELGAVLDLRAKYASVPMSLADACLVR
                     LTETLPDPIVVTTDSDFRIYRRHGRQVVPCLLPSGPST"
     gene            complement(4751..5008)
                     /locus_tag="AC16CY_05830"
     CDS             complement(4751..5008)
                     /locus_tag="AC16CY_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR3353336.1"
                     /translation="MKSLTIRLPDDLAERLERESRDRGVSKSDVVRERLSQNAPSPRV
                     ERGLREILEASWTARPAGRRRFRSDQKRKLADAIRAKKLPR"
     gene            5074..5574
                     /locus_tag="AC16CY_05835"
     CDS             5074..5574
                     /locus_tag="AC16CY_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HIR3353337.1"
                     /translation="MKSCIAPLVIAGLAAIPAVAAAQSPEQKRLEVMAGQWRIDIDQK
                     ATPLSPATKASGTEECEWFANRHVVCRGEAKGPAGTYGQMRTLSYVPARKQYAAYTVD
                     SLGTALVAYGQVSGDTWTFTTDQPSFNVRLTLKIAAGGYTALAEFAGPDGKYIPLSEV
                     KATRVK"
     gene            complement(5578..>6558)
                     /locus_tag="AC16CY_05840"
     CDS             complement(5578..>6558)
                     /locus_tag="AC16CY_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3353338.1"
                     /translation="IASLVEAWRWTPADHLLLVLPLHHVHGIINGLGSALAVRATCEI
                     LPSFDADAVWARLASGEVTVFTAVPTIYNRLIACWESAPPDVRHRWSQGAASLRLMMS
                     GSAALPVRTLERWRDISGHTLLERYGMTEIGMALSNPVAGERRPGFVGSPLPDVEVRL
                     VDESGAEVADGTPGELEVRGPAVFLEYWRRPDETRAAFRDGWFRTGDVALVENGSFRL
                     LGRTSIDIIKTGGFKVSALEIEEVLRTHPAIAECAVVGVSHEDWGECVSAAVELRDGA
                     SLSLDDLQQWAKSYLAPYKIPRALRQVPELPRNAMGKVVKPDVAGLFQLR"
BASE COUNT         1108 a         2239 c         2164 g         1047 t
ORIGIN      
        1 ggctgatgac gaccccgccc gggaccggca gcgcgccgcc ggacagtccc gtcccatgcc
       61 cccgcgcgac gaagggaagg ccctcgcggc tgcacagccg cacgatggcc tggacgtcgg
      121 ccgccgaacc aggcagtacg accagtgcgg gcgtttcgcg gagatgcgga agcgcgtcgc
      181 actcgtaggt cagaagctgc accggctcgg tgagcacggc atcgcgaccg acgatgcgcc
      241 tgaactcgtc gagcagccgg tcgtccatga gccctccgat ggtacactct ccgcgccagg
      301 cgcgcgtgcc gcgcctcgtt gcccgaggaa acgcgtgcga gtacgactgg actacggaac
      361 cgaaggactg gacgtcgacc tcccgggcga gcggacgacg atcatcgagc cgatggcgag
      421 gccggcggtt gccgacgcac gcgcgacgct gctccaggcc atccgctcgc cgctcggccg
      481 tccgccgctt cgcgatctgg tctcagcagg gcagaaggtc gccatttcgg tctgcgacat
      541 cactcgcgct cagccgcgga aggaaacgat cgaggcgctc ttcgaagaga tgccacaggt
      601 tgacccgaag gacgtgacga tcctgatcgc gactggaacg caccggacca acaccgatca
      661 ggagctcgag cgcatgctcg gccgcgagat cctgggccgg tgccgcgtga tcaaccacaa
      721 cagccgcgag aggtccacgc tgtcacgcgt gggcgccacg tcaaccggcg tcgaggtgta
      781 cctgaatcgc gaatggctcg gcgccgatgt gcgaatcacc accggctttg tcgagccgca
      841 tttcttcgcc gggttcagcg gcggcccgaa gatggtggcg ccgggcctcg ccggcctcga
      901 caccgtcatg acgctgcacg acgcgcgcca catcggccat cccaaggcga catggggcat
      961 caccgaggga aacccgattc acgacgacgt gcgggagatc gcacggatgg tgaccgtgca
     1021 tttcgccgtg gacgtcacgc tcaaccgcga gcagaagatc accgccgcct tcgccggcga
     1081 catctttgcc gagcatcgtg cggcctgcgc ctacgcgaag gacacggcga tgcgccaggt
     1141 gccgtctccc ttcgacgtgg tggtgacgac caactcggga tacccgctcg accagaacct
     1201 ctatcaggcg gtcaaaggga tgtccgcggc agccaagatc gtcaagccgg gaggcaccat
     1261 catcgccgcg gccgagtgcc gcgacggcct cccgtcccac ggctcctacg gagcggtgct
     1321 ggcgtcggcg gccaaccctg aagcgctgct cgcgatgatc tgcgcaccgg ggtactcggt
     1381 gccggatcaa tggcaggtgc aggttcaggc gcagatccag accaaggcca aggtgctcgt
     1441 caagaccgct ggtctcgggc cgagcgagat ccgcgccgcg cacttcacac cgatcgacga
     1501 cgtttctgag gccgtccggg catcgctcga tcgggcaggc caggggtcca cgctgtgcgt
     1561 gctgccgcaa ggaccgcaaa cgatccccta cctggcgtcc gaggcctaat ggtacgagtg
     1621 tgagcgaccc caagaacatc ctcctcgcgg ttctcgtcgc cgtcaccgtc gtcttcatcg
     1681 ccttctggtc acgggcgtcg tggcgccacc tggcgatcgg agcggtcacc aacttcttcg
     1741 acaccctggg catcgggtcc ttcgccacca ccacgacgat gtaccggctg tggcggctgg
     1801 tgaaggacga gaacattccc ggcacgttga acgtcggaca cgcgctcccg accgtcgcgc
     1861 aggcgctcat ctacatcacg atcgtggagg tcgattttcg aacgctgatc ctgctgatcc
     1921 tgtcggccgt cggcggatcg tggatcggcg cggccatcgt cgcggggctt ccgcgggccg
     1981 cgattcagcg tggcatgggc gtcgcgttga tggtcgcagc cgtgctgatg gtgatgacgg
     2041 cgctcgatcg cttcccgggc ggcggcactg cgctcgagct gacggccggc aagctcgtcg
     2101 tggcgtgcgc gatcaatttt gcgctgggag cgctgatgac gctcggcatc ggcgcctacg
     2161 cgcccatcat gatcatggtg agcctgctcg ggatgaatcc cacggcggcg ttcccgatca
     2221 tgatggggtc gtgcgcgttc ctgatgccga tcgccggtta ccagttcatc cggaagagcc
     2281 ggtacgacct ggccgcgtcg atcggcctga ccatcggcgg aatcccggcg gtgctggcgg
     2341 ccgcgctcat cgtgcggtcg ctccccctct actgggtgcg ctggctcgtg gtggtggtgg
     2401 tggtctatgc cgcgatctcg ctgctgaact cggcacggca cgagggacgt aagcggtagg
     2461 ccgtttcctc tgccccctgc cccctgctcc ctgtcccctg ccccctgccc ccttcagtgt
     2521 gtcatcgcgt ggatgacgac atcgatgccg agggcgtatc cagccggcga gaactgatag
     2581 aagaaggcgg ggtcctcgcc ttcgcgttcc cacgagtcgg caatgtccgt gttgtgcgtc
     2641 atcaccacca tgacgcggcg atggtggtcg cggatgaccc gcaggtgcac ctcggcgctg
     2701 tcggggcccc gttcggacgt ctccgcccct ccccacctgc gccagaactg gatgttcgtg
     2761 atctgcggaa cctgcttgat ctcgtacatc gttctgagca tcggatcgga gagcggcaca
     2821 tcctcgattc ggtactccgg tggctgcaga acgctggtga tctgcgaggc ccagtgatcc
     2881 cacgcctcag ggccccagaa gtcgtccacc cacaggaaac cgcccttcag caagtacgtc
     2941 cgcagccgcg cgccttcgtg ctcgtcgatg ccgatggtgc cggcatccga cgccaccacg
     3001 atcgggcaat tgaacaacgc atcgtcggtg aaccgcacga cgtagaagtt cggctggcgg
     3061 ctttcgtcca ggctgacgcg cgccttcgtc agctcggaca gccgcgtcgt gaggttgact
     3121 tcggcgtacg ggtagtccgt ctgccagccg atcccggagg gctccgtgcg caccgaccgg
     3181 tacgccagcc ggcagaccac gaaggaggcg tccggcatct gcgggggcgc ccatctcggc
     3241 gcgaaactgc cttcgcggaa gaagcgttgg gcctcgaccg ccccggccgc cgccaggagc
     3301 gcgacgcacg tgacgacggc ggcaatgctc gggcggggga gcagacccat gccgtgcagg
     3361 ttaacgcgaa ttgaccagcc cggcaacgtc gctctagctt ttcccggcct cccgggcgag
     3421 taggctcgat acctcgtcgg gttcgtcgaa accgggacgc acgaacgcgc gcgccctggg
     3481 aaacactcgt tcgagcgcct cgccgagcca caccaggtcg gcgaccaacg gcgccgcctt
     3541 cgaatccgac gtaacacccc gctcaaccgc gtcgagccac gccgcatgag agcgatcgat
     3601 gccgagcagc gcgactttcg ccgacccgtc gtggtcggcc ggccactcgc gctcatccgg
     3661 gtcgtcctcc gccagtccgc gaatcgcccg atgaatcttg accgcgatct gcaggtggaa
     3721 ccacgcgatc accgcgcgcg ggtcgcccgg atcgtggcct cggtccgggt gctcgcgcag
     3781 ccacgcacgt gcacgagtgc agtagtcgtc cgcgcgcgcc tcgatcaccc ggtgctccgg
     3841 tgggatcggc cgcggcggcc gcaactccgc agtcgccgcg gcgccgttcg cggcagcgtt
     3901 catctcatcg atcatttcct gcatccaggc cggcggaggc ggtggctggt cctgcggcag
     3961 cgggggcgcc tcgacgatct ccttcaagcc cggatcgagc ctggcctcga tctccgcgac
     4021 gtcggcaaac acgcgacagc gtgacgtgaa cgcgcacgtc tcgcaccagc gatcgcagta
     4081 gttgaagatg ccgacgatga atccgtcccg aacctccatt gcgtgacctc gccgtcatcg
     4141 ccgaggagca atcggcatgc ccgccgcggc ggcgccatca agagatcgcg cgaacgcagc
     4201 accgccagaa acgcccggga ttctcggcat cgtcctcccc tgcccgagga gggcaggatg
     4261 ccgatcgtgt cagcgatacc gagcgccggg aacgtcgttc ccgccgattc gtagtgacta
     4321 ctccgggccc ccacacgcga cgccggcgtc tcaggtcgaa ggtccggatg gcagcagaca
     4381 cggcacgacc tgccgaccgt gccgccggta gattcggaaa tccgagtccg tcgtgacgac
     4441 aatcggatcc ggcagcgtct cggtcaggcg cacgagacac gcatcggcca ggctcatggg
     4501 tacgctcgcg tacttcgcac gcagatcgag cacggctccg agctcctcgg cgaggtcgag
     4561 cgccaactgg aggtgctctt gccggagcaa ttggtcgagc gccgcggcct gtggctgccg
     4621 cagcagatac aaggcttcgg acagaaccga ttcgcaggtc aaccagggtc tcggctgtcg
     4681 cggcagcgtc tcccgtgccc aggtgtgatg cgcctcacgg ggatcgagca gggcgaccag
     4741 cgcgccgctg tcaacgaggc agcttcttgg cacggatcgc gtctgcgagc tttcgtttct
     4801 ggtcggaacg gaatcttcgc cgacctgcag gcctggctgt ccacgatgct tcgaggatct
     4861 ctctgaggcc gcgctcgact cgtggcgacg gcgcgttctg actcaggcgc tcgcgaacga
     4921 cgtcggactt ggacacaccg cggtcgcgcg actctcgttc aagccgctca gccagatcgt
     4981 cagggaggcg aatcgtcaac gacttcatca cgacatcgta agacactcaa ggcagttatg
     5041 caagacgacg gttcgtatgt tagtctccgt gtcatgaagt catgcatcgc ccctctcgtg
     5101 attgccggcc tcgccgcgat tccggctgtc gctgccgcgc agagccccga gcagaagagg
     5161 ctcgaggtca tggccggcca gtggcgcatc gacatcgacc agaaggcgac accgctgtca
     5221 ccggcaacca aggcgtcggg caccgaggag tgcgagtggt ttgccaaccg gcacgtcgtg
     5281 tgtcgtggcg aggccaaggg cccggcaggc acctacggcc agatgcgaac attgagctac
     5341 gttcccgcgc gcaagcagta cgcggcgtac accgtcgaca gcctgggcac tgcgctggtg
     5401 gcatacgggc aggtcagcgg cgatacctgg acgttcacca cggaccagcc gtcgttcaac
     5461 gtccggttga cgctgaagat cgccgcgggc gggtacacgg ccctcgcgga gttcgcggga
     5521 cccgatggca agtacatccc gctctcggag gtcaaggcca cgcgcgtgaa gtgacgatca
     5581 gcgcaattgg aagaggccgg cgacgtcggg cttgacgacc ttccccatcg cattccgcgg
     5641 cagctccgga acctgccgga gcgcacgcgg aatcttgtat ggagccagat aggacttcgc
     5701 ccactgctgc agatcgtcga gcgacagcga cgcaccatcg cgaagctcca cggcggccga
     5761 cacgcactcg ccccagtcct cgtggctgac accgacgacg gcgcattcgg caatggcggg
     5821 gtgggttcgc agcacctcct cgatttcgag cgcggacacc ttgaagcccc ccgtcttgat
     5881 gatgtcgatg ctggtccgtc ccaacaggcg gaacgatccg ttctcgacga gagcgacgtc
     5941 gcccgtgcga aaccagccgt cacggaacgc cgcgcgcgtc tcgtcggggc gccgccagta
     6001 ttcgaggaag acggccgggc cgcgcacctc cagctcgcct ggcgtgccgt cggcgacttc
     6061 ggcgcctgat tcgtccacga gcctgacttc gacatccggc agcggagagc cgacgaatcc
     6121 gggccgcctc tcgcccgcaa ccggattgga cagcgccatg ccgatttcgg tcatgccgta
     6181 ccgctcgagc agcgtgtggc cgctgatgtc ccgccagcgt tcgagcgtgc gaaccggcag
     6241 cgcggccgag ccggacatca tgagccgaag cgacgccgcg ccttgcgacc agcggtgacg
     6301 cacgtcgggt ggagcggact cccagcacgc gatgagacgg ttgtagatgg tcggcacggc
     6361 ggtgaagacg gtcacctcgc ccgaggcgag ccgggcccag accgcgtcgg cgtcgaagga
     6421 cgggaggatc tcgcaggtgg cgcgcacggc gagggccgac ccgagcccgt tgatgatgcc
     6481 gtgcacgtgg tgaagcggga gcaccaggag caggtggtcg gcgggcgtcc agcgccaggc
     6541 ctcgacgagc gacgcgat
//
LOCUS       DBNGSH010000254         2280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4434428, whole genome shotgun sequence.
ACCESSION   DBNGSH010000254 DBNGSH010000000
VERSION     DBNGSH010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4434428"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1937)
                     /locus_tag="AC16CY_05845"
     CDS             complement(<1..1937)
                     /locus_tag="AC16CY_05845"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR3353339.1"
                     /translation="MSTAKIIYTHTDEAPALATRSLLPIIRAFTRAAGVTVETRDISL
                     AGRILASFPERLTAGQRQADDLAELGALAKTPDANIIKLPNISASIPQLKEAIAELQS
                     QGYGVPDYPDDPRNDAEKAIKATYARVLGSAVNPVLREGNSDRRVAASVKRYAKTHPH
                     SMGAWSSGSNTHVAHMSGGDFYANEQSAVIAADGSLRIEFADSSGRRAVLKESVKVSA
                     GDIVAASVMSRSALTDFFTKAIDEAKAAGVLLSLHLKATMMKVSDPIMFGHAVRTYYK
                     DVFAKHAAAFDSLGVDPDNGIGEVYSKIEALPAAQRSAIEADLQAVYSTRPALAMVDS
                     SRGITNLHVPSDVIIDASMPAAIRASGQMWGPDGKLHDTKAMIPDRCYAGIYQATIED
                     CKQHGAFNVSTMGSVSNVGLMAQQAEEYGSHDKTFEIASKGTVRVADAGGRTIMEHVV
                     DAGDIWRMCRTRDLPIRDWVRLAVARARATGHAAIVWLDDKRAYDRNVIEKTVRYLKD
                     HDTSGLDIRIMPPVEAIRATLARVRAGQDTISVTGNVLRDYLTDLFPILELGTSAKML
                     SIVPLLDGGGLYETGAGGSAPKHVQQLLQENYLRWDSLGEFLALAVSIEDLGRKTGNA
                     RALVVADALDEATGKILDNDKS"
     gene            2048..>2280
                     /locus_tag="AC16CY_05850"
     CDS             2048..>2280
                     /locus_tag="AC16CY_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HIR3353340.1"
                     /translation="MGSASDWDTMKAAVEVLDRFGVPNERHVMSAHRTPVETAAFASG
                     AEARGIEVIIAGAGGAAHLAGVVAAHTLVPVLGV"
BASE COUNT          312 a          757 c          781 g          430 t
ORIGIN      
        1 gacttgtcgt tgtcgaggat cttcccggtg gcttcgtcga gcgcgtcggc cacgacgagc
       61 gccctggcat tgccggtctt ccggccgaga tcctcgatcg acacggcgag cgccaggaac
      121 tctccgagcg aatcccatcg caggtagttc tcctggagga gctgctgcac gtgcttcggc
      181 gccgatccgc cggcgccggt ctcgtagagc ccgccaccgt cgagcagcgg cacgatcgac
      241 agcatcttcg cgctcgtccc gagctcgagg atcgggaaca gatccgtgag gtaatcgcgc
      301 aggacgttgc ccgtcacgga aatcgtgtcc tgcccggcgc ggacgcgcgc gagcgtggcg
      361 cgaatcgcct ccacgggcgg catgatgcgg atgtcgaggc cgctcgtgtc gtggtccttc
      421 aggtaccgca cggtcttctc gatcacgttg cggtcgtagg cgcgcttgtc gtcgagccag
      481 acgatcgccg cgtggccagt ggcgcgcgcg cgcgccactg cgagtctcac ccagtccctg
      541 atcggcaggt ccctcgtccg gcacatgcgc cagatgtcgc cggcgtcgac cacgtgctcc
      601 atgatcgtcc ggccgccggc gtcggcaacc cggacggtcc ccttcgaggc gatctcgaac
      661 gtcttgtcgt gcgaaccgta ctcctcggcc tgctgcgcca tcaggccgac gttcgacacg
      721 ctgcccatcg tcgagacgtt gaacgcgccg tgctgcttgc agtcctcgat cgtcgcctgg
      781 tagatgccgg catagcaccg gtcggggatc atcgccttcg tgtcgtggag cttcccgtcc
      841 gggccccaca tctgccccga ggcgcggatc gccgccggca tggacgcgtc gatgatcacg
      901 tcgctcggga catggaggtt ggtgatgccc cggctggagt ccaccatggc gagcgccggc
      961 cgcgtcgagt acaccgcctg gaggtcggct tcgatcgccg accgctgcgc tgccggaagc
     1021 gcctcgatct tcgagtagac ctcgccgatg ccgttgtcgg gatcgacgcc aagcgagtcg
     1081 aacgcggctg cgtgcttcgc gaagacgtcc ttgtagtagg tcctgacggc gtgcccgaac
     1141 atgatcggat cggacacctt catcatagtg gccttgaggt gcagcgacag cagcacgccg
     1201 gcggccttcg cctcgtcaat ggctttcgtg aagaagtcgg tcagcgcgct ccggctcatg
     1261 accgacgccg cgacgatgtc gccggcggag accttgacgg actccttcag tacggcgcgc
     1321 cgcccgctcg agtcggcgaa ctcgattctc aggctgccgt ccgcggcgat gacggccgac
     1381 tgctcgttcg cgtagaagtc gccgccgctc atgtgcgcga cgtgggtgtt cgagccgctg
     1441 ctccaggcgc ccatcgagtg cgggtgcgtc ttcgcgtacc gcttgaccga cgccgcaacg
     1501 cggcgatcgg agttcccttc gcgcaagacc gggttgacgg cgctgccgag cacgcgggcg
     1561 taggttgcct tgatcgcctt ctcggcgtcg ttccgcgggt cgtcgggata gtcgggcacg
     1621 ccgtagccct ggctctggag ctcggcgatc gcctctttga gctgcgggat cgacgcgctg
     1681 atgttcggca gcttgatgat gttggcgtct ggcgtcttgg cgagcgcgcc gagctcggcg
     1741 agatcgtctg cctggcgctg cccggctgtc aggcgctccg gaaaactggc gaggattcgg
     1801 ccggcgagcg agatgtcgcg tgtctcgacc gtgacgccgg cggcccgcgt gaacgcccgg
     1861 atgatgggca gcagcgaacg ggtggcgagc gcgggcgctt cgtcggtgtg ggtgtagatg
     1921 atctttgcgg tgctcatgtc tctccgggac ttcggtgcct cagaaccttc cattttccct
     1981 caagtcggcg tcgccgacat ttcgcggtag catttcgggc gtgaatcact ccctcgtcgc
     2041 cgtcatcatg ggttctgctt ccgactggga cacgatgaaa gccgctgtcg aggtgctcga
     2101 ccgctttggc gttcccaacg agcggcatgt catgtccgcg caccgcacgc cggtcgagac
     2161 ggcggcgttc gcctcgggcg ccgaggcgcg gggcatcgaa gtgatcattg ccggcgcagg
     2221 cggggcggcg caccttgccg gcgtcgtcgc ggcgcacacg ctggtgccgg tgctcggcgt
//
LOCUS       DBNGSH010000255         2166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2566760, whole genome shotgun sequence.
ACCESSION   DBNGSH010000255 DBNGSH010000000
VERSION     DBNGSH010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2566760"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="AC16CY_05855"
     CDS             complement(<1..1476)
                     /locus_tag="AC16CY_05855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3353341.1"
                     /translation="MTDPRWNRAKQIFHEALEKTGPERARVVAAACADDRELRAQVEG
                     LLRAHDEADGFLTSPTGGPDEAARTAARLAAAVPPEAPGTRIGPYKLLQVIGEGGFGI
                     VYMAEQEQPIRRRVALKIIKLGMDTKEVIARFEAERQALAMMEHPNIARVFDAGATDS
                     GRPYFVMELVQGVPITTYCDGNRLSTRERLDLFVGVCKAVHHAHEKGLIHRDIKPSNV
                     MVTLHDGTPVVKIIDFGVAKATNQRLTEKTLFTAYGQFVGTPAYMSPEQAEMSGLELD
                     RRSDVYSLGVLLYELLTGTTPFEAEVLRQRGFVEMLRVLREEDPPTPSARLSSLGDRL
                     IEVASRRHVEPAALARLVQGDLDWIVMKAMDKDRRRRYGSASELLDDVARYFRNEPVI
                     ARSPSAAYRMRKFASRRRTPLLAAASAAVALALGGLFVLTRPAPGAGATTSGVRPLPR
                     LDDADEMLALSPDGTKAAFMDYDKGQNLAVLDVMSQRITPLT"
     gene            complement(1480..2055)
                     /locus_tag="AC16CY_05860"
     CDS             complement(1480..2055)
                     /locus_tag="AC16CY_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3353342.1"
                     /translation="MAPDAGAVTRILAGLRGADREESLNRLFPVVYTELRAAARAQLR
                     FERADHTLQATALVHEAYLRLLGGSYPTWNDRQHFFRAAAEAMRRILVEHARRRARVK
                     RGGNPVRVSLTDVDSVAGHDPVEILALDAAIRRLEEQDPVAADIVRLRFFAGLSVEET
                     AGMLEVSERTVKREWAFARAWLYEALKGSKG"
BASE COUNT          340 a          762 c          704 g          360 t
ORIGIN      
        1 ggtcagcggc gttatccgct ggctcatcac gtcgaggacc gcaaggttct gccccttgtc
       61 gtagtccatg aaagcggcct tcgtgccatc cggggaaagg gcgagcattt cgtcggcgtc
      121 gtccagcctc gggagcgggc ggactcctga cgttgttgca cccgcgccgg gcgcaggtcg
      181 tgtgagcacg aagagcccac ccagcgcgag agcgacggcc gcgctggccg cagcaagaag
      241 gggcgtcctc cgccgcgacg caaacttgcg catccgatag gcggcgctcg gcgatctcgc
      301 gatcaccggt tcgttgcgga agtatcgcgc gacgtcgtcc agcagctcgg acgccgaccc
      361 atatcgccgg cggcgatcct tgtccatcgc cttcatgacg atccaatcca ggtcaccctg
      421 gacgagtctc gccagcgctg ccggctccac gtgccggcga ctcgccacct cgatgagccg
      481 atcgccaagc gaggagagtc gagccgatgg cgtcggaggg tcctcctcac ggaggacgcg
      541 cagcatctcc acgaacccgc gctggcgcag cacctcggcc tcgaacggcg tcgttccggt
      601 cagcagctcg tacagcagca cgcccagcga gtagacgtcg ctccggcgat cgagctccag
      661 accgctcatc tcggcctgct ccgggctcat gtaggccggg gtgccgacga actggccgta
      721 tgccgtgaac agcgtctttt ccgtgagccg ctggttggtg gccttggcga cgccgaagtc
      781 gatgattttc acgaccggcg taccgtcgtg gagggtgacc atcacgttgg acggcttgat
      841 gtcgcgatgg atgagtccct tctcgtgcgc gtggtggacc gccttgcaca cgccgacgaa
      901 gaggtcgagc cgctcgcgtg tgctgagccg gttgccgtcg cagtaggtcg tgatcggcac
      961 gccctgcacg agctccatca cgaagtacgg ccggcccgag tcggtcgcgc cggcatcgaa
     1021 caccctggcg atgttcgggt gctccatcat cgccagcgcc tgacgctcgg cctcgaaccg
     1081 ggcgatcact tccttggtgt ccatgcccag cttgatgatc ttgagcgcca cgcgccgccg
     1141 gatcggctgc tcctgctccg ccatgtagac gatgccgaag ccgccctccc cgatcacctg
     1201 caacagcttg tacggtccga tgcgcgtgcc cggagcctcg ggaggaaccg cggcggccag
     1261 ccgtgccgcc gttctggccg cttcgtccgg cccacccgtc ggcgaggtga ggaatccgtc
     1321 cgcctcgtcg tgcgcccgga gcaagccttc gacctgcgcg cgcagttccc ggtcgtccgc
     1381 gcaggcagcc gcgacaacgc gagcccgctc gggtccggtc ttctcgagcg cctcgtgaaa
     1441 gatctgcttc gcgcgattcc agcgtgggtc cgtcatgatt cagcctttcg aacccttcag
     1501 cgcctcgtag agccacgccc gcgcaaacgc ccactcgcgt ttgacggtcc gctccgacac
     1561 ctcgagcatc cccgccgtct cctcgaccga aagtccggcg aagaaacgca gtcgaacgat
     1621 gtcggcggca acagggtcct gctcttctaa gcgccggatt gcggcgtcga gggccagaat
     1681 ctccacaggg tcgtgcccgg ccaccgaatc gacgtcggtc aacgagaccc ggaccgggtt
     1741 cccgccccgc ttcacgcgcg cccgccgccg cgcgtgctcg accaggatgc gccgcatcgc
     1801 ctcggcggcg gctcggaaga agtgctgtcg atcgttccag gtcgggtagg acccgccaag
     1861 gaggcgcagg taggcttcat ggacgagcgc cgtggcctgc agggtgtgat cagcgcgctc
     1921 gaaccggagc tgcgcccgtg ccgccgcgcg aagctcggta tacacgacgg gaaagagccg
     1981 gttcagcgat tcctcgcggt cggcccctcg caggccggcg aggatccgcg tgacagcgcc
     2041 agcgtcaggt gccatcagct ccctcaggtc gcgacacgtc ctgccgtcgc gatcttggac
     2101 gcctccgccg ggcgagtcaa ggggcgcaat gagcaggttc gcacacctgc acacacaggg
     2161 tctttg
//
LOCUS       DBNGSH010000256         4119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3925207, whole genome shotgun sequence.
ACCESSION   DBNGSH010000256 DBNGSH010000000
VERSION     DBNGSH010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3925207"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1357)
                     /gene="hemA"
                     /locus_tag="AC16CY_05865"
     CDS             complement(26..1357)
                     /gene="hemA"
                     /locus_tag="AC16CY_05865"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR3353343.1"
                     /translation="MHLFLLGVSHRTAPVDLRERLDFASRDLGAAVEALATRPSAAES
                     VVLSTCNRSEIYLASSDPGRAREDVTSFLSEYHDLPVSAFTPHLFSHDNEAAVRHLFR
                     VAAGLDSLVVGEPQILGQVKDAFQTAADRRCTGPTLSKLFHWSFVVGKRVRTETALGE
                     GAVSIGFAAVALARKIFGQLDGRRVLVIGAGEISTLTAQHLRANGAGEILIASRTPAH
                     AEALAATVEGRTLAWDDLMQGLSTADIVVTATGSQRPIVTRAQVETVIGWRRSGPLFV
                     IDLAVPRDVEASVGDIEQVFLYNVDDLLSVVQENLTRRSSEIERAEIIVSQELAKFVA
                     WQRSRSAVPTVVALRERFDRVRRSELQRLDGKLSSLTPEQKGRVEEVTRLIVEKLLLE
                     PTEQLKGLPDEETQSAYTEAINRLFRLREGEPVPAPDEGASEVTPADRRGK"
     gene            complement(1459..2292)
                     /locus_tag="AC16CY_05870"
     CDS             complement(1459..2292)
                     /locus_tag="AC16CY_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR3353344.1"
                     /translation="MNLLPLALYAAAGVVYAIHFAKRQPAVGRTASTLLLLAALAHTF
                     VVGMQTMEVWHVPLANPSRAVSSFVWLLALSYLYLELTTDERAMGVFILPILVGLQII
                     PVVYPGFENKDPVLDSPWFWVHVASLLFAYASFALSAMLGLTYVLQFKEIKKKHLGYF
                     YTRLPSLHVLDAMNSRAVAVGWLFLTIGVVVGIVWTAQARSLAPDNSNLQAISLLDPK
                     VFIVMVTWGVYSFSLFARKTLGWTGRRAAWLSTAGFATVLLNLLPVSYFITTSHTFSG
                     P"
     gene            complement(2289..3203)
                     /locus_tag="AC16CY_05875"
     CDS             complement(2289..3203)
                     /locus_tag="AC16CY_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3353345.1"
                     /translation="MKRWTLLTALVIVGLAPRAAGQQQGAFRAGVDVVSLTVTVTDSS
                     GHYVTDLDEAEFTVFEDGVKQKVTYFSRRQQPIALSLLLDSSASMEDHLPTLQAAASS
                     FVHRLKPNDISQIIDFDSKVEIRQTFTGNQADLDGAIERLVAGGSTSLHNAIYIALKE
                     LRKVKAVSEEDVRRQALIVFSDGEDTSSLVSFEEVLDLAKRSETSIYTIALRGSDVQA
                     KGFREAEFIMRTLAQETGGRAFFPGRIEDLTGVYGQIADELASQYTLGYTSGNPRRDG
                     AWRRVLIQLTRPNITPRTKRGYYAPTAR"
     gene            complement(3205..>4119)
                     /locus_tag="AC16CY_05880"
     CDS             complement(3205..>4119)
                     /locus_tag="AC16CY_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3353346.1"
                     /translation="DRWLALPRSLLLMAAAMAAVATLATERSAAQAARATREGTMYVS
                     AVNREGEPVTGLGPEDFIIREDGARREVLRVSKATEPIDLAILLDNSVGATNGVTFLR
                     ESLTNFVAKMSPGNQMALIGLADRPTIIVDYTEDTKRLTEAVGRLFPLPASGMTLLDA
                     IYETSQGLRRRQGPRAVMVAVFTDSVEFTNRYEKDVVRALLQVDAQLHMVTIGVFPHR
                     DDEHSLRERSFLLDSGPRESGGNRISLLAPHAMNNAMQRVARELSEQYKVVYGRPESL
                     IAPERAVVSSAKADVTMRGSRARGTSGG"
BASE COUNT          694 a         1366 c         1357 g          702 t
ORIGIN      
        1 gtactcttcc agttgatacc gtccattact tgcctcgacg atcggccggc gtcacctcgc
       61 tggcgccttc gtccggggcc ggcacgggct cgccttcccg caggcggaag aggcggttga
      121 tagcctctgt gtaggcggac tgcgtctctt cgtcgggaag ccccttcagt tgttcggtcg
      181 gctcgagcag gagcttttcc acgatcaggc gcgtgacttc ctcgacgcgc cctttctgtt
      241 cgggggtcag gctggacagt tttccatcga gccgctgaag ctcggatcgg cgaacccggt
      301 cgaaccgctc gcgcaacgcc acgaccgtcg gaacggcgct tcgtgaacgc tgccaggcca
      361 cgaacttggc cagttcctgc gacacgatga tctcggctcg ctcgatctcc gagcttcgac
      421 gcgtgaggtt ctcttgcacc acggacagca ggtcgtccac gttgtacagg aacacctgct
      481 cgatgtcgcc gaccgatgct tcgacgtccc tcggcaccgc caggtcgatg acgaagaggg
      541 gtccggaacg cctccacccg atcacggttt ccacctgcgc gcgcgtcacg atgggccgct
      601 gggagccggt tgcggtgacg acgatgtcag cggtcgaaag cccctgcatc agatcgtccc
      661 aggcgagcgt ccggccctcc acggttgccg cgagcgcctc ggcgtgagcc ggtgtccggc
      721 tggcgatcaa gatctcgccg gcgccgttgg cccgaaggtg ctgcgctgtc agggtgctga
      781 tctctcccgc accgatcacg agcacgcggc ggccgtcgag ttgcccgaag atcttgcgcg
      841 cgagcgcgac ggcggcaaag ccgatcgaga ccgccccttc gccgagcgcc gtctccgtcc
      901 tgacgcgctt gcccacgacg aacgaccagt ggaacagctt gctcagcgtc ggaccggtgc
      961 agcgccgatc cgccgccgtc tgaaacgcgt ccttgacctg cccgaggatc tgcggttccc
     1021 cgacgaccag cgagtcgagg cccgcggcca ccctgaacag gtgcctgacg gcagcctcgt
     1081 tgtcgtgaga gaacaaatgc ggcgtgaacg cggagaccgg caggtcgtgg tactcgctga
     1141 ggaacgaggt gacgtcctcg cgcgcgcgtc cgggatcgct gctcgcaagg tagatttccg
     1201 agcggttgca cgtcgagagc acgaccgact cggcggcgga cggccgcgtc gccagcgcct
     1261 ccacggctgc gccgagatcg cggctcgcga agtcgagccg ctcgcgcaga tcgacgggcg
     1321 ccgtccggtg actcacgccg agcaggaaca agtgcatcta ctggggccgt ccttcgattc
     1381 gtaaagcggg ccgctccgtt gtcgcggccc gagaaattac ggtcgatcgc tcgttgcgga
     1441 ttctgcagct tctgtttgtt acggtccact aaacgtgtgg ctggtcgtga tgaagtagct
     1501 caccggcagg aggttgagca ggacggtggc gaacccggcg gtcgagagcc acgccgcccg
     1561 cctgccggtc catccgagcg tcttccgcgc gaacagcgaa aacgagtaca caccccaggt
     1621 caccatcacg atgaacacct tggggtccag cagcgagatc gcctggagat tggagttatc
     1681 cggcgccagg gacctggcct gtgccgtcca gacgatgccg acgaccacac cgatcgtcag
     1741 aaacagccag ccgacggcga ccgctcggga gttcatcgcg tcgagcacgt gaagcgacgg
     1801 cagcctcgta tagaagtagc cgaggtgctt cttcttgatc tccttgaact ggagcacgta
     1861 ggtcagcccc agcatcgcgc tcaacgcgaa gctcgcgtag gcgaagagca gcgaagcgac
     1921 gtgcacccag aaccaggggc tgtcgagaac cgggtccttg ttctcgaagc ccgggtagac
     1981 caccggaatg atctgcagcc ccacgaggat cggcaggatg aacacgccca tcgcgcgctc
     2041 gtccgtcgtc agctcgaggt acaggtacga gagcgcgagc agccagacga atgacgacac
     2101 ggccctcgac gggttcgcga gcggcacgtg ccacacttcc atcgtctgca tcccgaccac
     2161 gaaggtgtgg gcaagcgcgg caagcagcag cagcgtggac gccgttcgac cgacagcggg
     2221 ctgccgcttc gcgaaatgaa tggcgtagac gacgccggcc gcggcgtaca acgcgagagg
     2281 aagaagattc aacgggccgt cggggcgtag tagcccctct tcgtgcgcgg ggtgatgttc
     2341 ggccgcgtca actggatgag aacgcggcgc cacgcaccgt cgcgacgcgg attgccggac
     2401 gtgtatccca gcgtgtactg gctggcgagt tcgtccgcga tctggccgta cacgcccgtg
     2461 aggtcctcga tgcggcccgg gaagaacgcc cggccgccgg tctcctgggc gagcgtacgc
     2521 atgatgaact cggcttcccg gaagcccttg gcctgcacgt cgctgccccg gagcgcgatc
     2581 gtgtagatcg acgtttccga tcgcttggcg agatccagca cctcctcgaa cgacacgagg
     2641 ctggaggtgt cctcgccgtc ggagaagaca atgagcgcct gacggcgaac gtcctcctcc
     2701 gacaccgcct tcaccttccg cagttccttc aacgcgatgt agatggcgtt gtgaagcgac
     2761 gtggagccgc cggccaccaa ccgctcgatc gcgccgtcga gatcggcctg gttgccggtg
     2821 aacgtctgcc gtatctcgac cttgctgtcg aagtcgatga tctgggagat gtcgttcggc
     2881 ttgagccggt gcacgaagct cgacgcggcc gcctgcaggg tgggcaggtg atcctccatg
     2941 ctcgcgctgc tgtcgagcag cagcgacagc gcgatcggct gttgacgcct ggagaagtac
     3001 gtgaccttct gcttgacccc gtcttcgaac accgtgaact cggcctcgtc gaggtcggtg
     3061 acgtaatggc ccgaactgtc agtcaccgtc acggtcagcg agacgacgtc tacgccggcg
     3121 cggaacgcgc cttgctgttg tccggccgcc ctcggagcga ggcccacgat gacgagcgcg
     3181 gtaaggagcg tccagcgctt catgtcagcc tcccgacgtt ccccgagccc gcgacccgcg
     3241 catcgtgacg tccgcttttg ccgaagacac gacggctcgc tcgggcgcga tgagcgactc
     3301 cgggcggccg tacaccactt tgtattgctc ggacagttcg cgcgccaccc gctgcatcgc
     3361 gttgttcatg gcgtgcggcg cgagcagcga gatgcggttt cccccgctct ctcgcggccc
     3421 cgagtccagc agaaacgagc gctcgcggag ggagtgctcg tcgtcgcggt gcgggaacac
     3481 gccgatcgtg accatgtgca gctgcgcatc gacctggagc agcgccctca cgacgtcctt
     3541 ctcgtaccgg ttggtgaact cgacgctgtc ggtgaacacg gcgaccatga ccgcgcgcgg
     3601 gccctggcgg cgcctcagtc cctggctcgt ctcgtagatc gcgtcgagca gcgtcatgcc
     3661 gctcgccggc agcgggaaca accggccgac ggcctcggtc agacgcttcg tgtcctccgt
     3721 gtagtccacg atgatcgtcg gccgatccgc caggccgatg agcgccatct ggttgccggg
     3781 tgacatcttc gcgacgaagt tcgtgagact ctcgcggagg aacgtcacgc cgttggtggc
     3841 gccgacgctg ttgtccagca ggatcgcgag atcgatgggt tcggtggctt tcgagacgcg
     3901 gagcacttcg cgccgggccc cgtcctcgcg aatgatgaag tcctccggtc cgagcccggt
     3961 gaccggctcc ccctcgcgat tgacggcgct gacatacatc gtgccttcgc gagtggcccg
     4021 ggcggcctgc gccgcgctgc gctccgtcgc aagcgtggcg acggcagcca tggcagcggc
     4081 catcagcaga agggagcggg gaagggcgag ccagcgatc
//
LOCUS       DBNGSH010000257         8151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2566986, whole genome shotgun sequence.
ACCESSION   DBNGSH010000257 DBNGSH010000000
VERSION     DBNGSH010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2566986"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2023)
                     /locus_tag="AC16CY_05885"
     CDS             complement(<1..2023)
                     /locus_tag="AC16CY_05885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3353347.1"
                     /translation="MTSRLVQLLTARRFAPLFITQFLGAFNDNLLKSALGIAVTYRIA
                     GQTGMDAATLVMIAGGLFIAPFFLFSGASGTLADRTDKAAIARWVKVAEIAIMLLGAL
                     GLARQSVPLLLASLFCLGTHSTVFGPIKYALLPQHLRDDELVAGNALIEAGTFLAILF
                     GTILGGSLVLVDNGALIVGACGLVSAAGGWLASRQIPPAPPEPGSTTPRPHIWRDSVA
                     VVRHVTGRSELLLPILAVSWFWLFGATVVSGLPVLAKDVLFANERVVTLMLALFAVGI
                     GIGSMLAERLLHGEVSARYVPLAALAMGLFAIDLHLASARLVPASELASVTAFVSAPG
                     NWRILADLVGIAVAGGLFCVPLYALLQHESEPQHRARVIAANNIINALAMTLAAVAAA
                     VLLARGLTMGELFAVGGLATIPVAAVAAWVVRRGLAKLVMRTVLRLLYRVRVDGLEHA
                     RAAMPHAVIAANHASFLDGLLLGAFLPGDPIFAVDTFIARKWWARPFLALVNAVPVDP
                     TNPLSIRSMIRAVEAGSACIIFPEGRITTTGSLMKVYEGPAVIAERTRAALVLVRIEG
                     AELTPFSRLAGKVRTRLFPRISIRILPPRRLTSPDGVVGRARRVALRRALRDEMVKSA
                     FDTARIDMTLFDALLDARRLHGGGHVIADDIEQRPMTYRGLIAASYALGA"
     gene            complement(2036..2611)
                     /locus_tag="AC16CY_05890"
     CDS             complement(2036..2611)
                     /locus_tag="AC16CY_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3353348.1"
                     /translation="MPRHRFTDTRDRIEKAAIRLFVRKGVAETTVRDIARAVGVSEGA
                     LYRHFTSKEELVWQAFERHYVEFAGRLEALAASEPTTRGKVGAMIHGFCRAHDENQEL
                     FRFLLFVQHGQLGKLEPDTPTPVRAVRAVMSRAIASGDIPAQSPDLATAVLFGVVLEP
                     VQFAAYGQLPSQMSSMCDRLTAAAWAAVTTV"
     gene            complement(2744..3982)
                     /locus_tag="AC16CY_05895"
     CDS             complement(2744..3982)
                     /locus_tag="AC16CY_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3353349.1"
                     /translation="MTRPFEPKFMKVGVLTAALQELTPREARDADPDLAIEEWLQFAR
                     ELGAGYIQLSAALHPSETDVPPEAMLDPVANTLDLRQPFDKARARRVEAAVKSTSVAF
                     SDLAYFDNLLHHDAATRRKKHEFLLRVFDAAVLLGVNAVCGFVGRNQQRSMDQNLVDF
                     EEQFVPLLEAAKARGLTYRVEQCPMPGWTTSDNWHNNIAYTPGAWIALHRICERHGVG
                     DQFRIHYDPSHAILMGQDTRSVFQYLKDTGYGFLIGGFHVKGQVIDSKGVAAWGYGGQ
                     TMERGDWVGGKASDNPADQVNAWKKQVVLCEHELPGTARHDPLAYLQNRTVDWLDHQL
                     AARELLQLDIPNTHLVVEHEYPAARIQDREKLKPILQGSLAFVRRIDEAAACMYALQH
                     EVLSSQGIPVQGFGREAYRS"
     gene            complement(3979..5133)
                     /locus_tag="AC16CY_05900"
     CDS             complement(3979..5133)
                     /locus_tag="AC16CY_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3353350.1"
                     /translation="MTRVGMGLVGAGFVGPHHIDAVRRLGFVDVVAIATSNEASARKK
                     ADALGVRKAYGRYQALIDDPEVQVVHNATPNVLHYEVIAAALARGKHVVADKPLAMTA
                     EQSKRLVEQARQAGVVTAVTFNYRGNPLVQQARLAVARGDIGVPHFLHGYYLQDWLIK
                     DTDYSWRLDPKQSGASSAIGDIGSHWCDLAQHVSGLEITHVLGDITTVVPKRKKPRGS
                     REAFQAGGAGAVDVVDIEVEDLASVLLRFENGAKGAFSVGQVCGGHKNDLVLEVCGPK
                     ASVRWRQEHQNELWIGHRDKANEVLQKDPALVDDAVRGYASLPGGHQEAWADAFRNLM
                     FDIYGCIAAGKPYRALPPAVATFEDGYRANRIVDAILESARKGGVWTQVR"
     gene            5213..6073
                     /locus_tag="AC16CY_05905"
     CDS             5213..6073
                     /locus_tag="AC16CY_05905"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HIR3353351.1"
                     /translation="MNRRDFVLALAGPLAIAQVGRAQGAAPARLKGRIRQGVTRGVFA
                     RGTPLEDACREAARLGITGFDLIGPADWPTLRKYGLVPSMYPPGPGGTIPDALNRREN
                     HAKLEASLHAAIDECAANKVPNIITFSGNRRGMSDAEGADNCVAFLNRIKAHAEDKGV
                     TISIEILNSKVNHRDYMFDHMPWGVDVVTRVNSPRVRILYDIYHAQIMDGDVVRTIRD
                     HIGLISHFHTGGNPGRNDIDETQELNYRYVMQEIAALGFTGFVTHEYTPKAGSDPIAV
                     LRKAVEICDV"
     gene            6066..6839
                     /locus_tag="AC16CY_05910"
     CDS             6066..6839
                     /locus_tag="AC16CY_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3353352.1"
                     /translation="MSRTSAAFAIVLLVATGSLAAQGGPYQRQRIDTAAADRGRAVYA
                     RLCINCHGSTAKGTERGPDLIRSATVLRDRLGNLIGPALRTATHRSGVTETQVVDLSH
                     FLHERVEATARNRTPTAPLNVLTGDPEAGREYFNGAGTCSACHSSTGDLAGIATRITD
                     PVNLQQRFLFPTLRRDGARRVEVTVAPASGPAVSGALVRMDDFSVSLRDASGEYHSFS
                     RVPGTTVSVRNPLAAHHELLDRYTDTHMHDITAYLSTLK"
     gene            6836..>8151
                     /locus_tag="AC16CY_05915"
     CDS             6836..>8151
                     /locus_tag="AC16CY_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3353353.1"
                     /translation="MKTIHLRAGRYGGHVITLAAAVLAAAALGAQGTLAPAELLRPLG
                     AAWPMYNGDYSGQRFSALSSIDTSNVSSLTLAWVHRPNAGAAPAGGGGATAVTIKGTP
                     VVVNGVLYVTIPDHVWALDARTGREIWHATWPSKGGWHIANRGVAVLGSTLYVETPDC
                     NLVALDVRDGKEKWRTEICDLEQFYYASTAPLVVGRHVIVGVSGDDLDIPGYLQAHDP
                     DTGARQWRWYTYPEPGTPEAKTWPSVEAMMHGGGMTWGSTTYDPELNLIYFGTGNPQP
                     VINGRKRQGDNLYTESIVALNPDTGKLAWYFQSSPHDTHDWDATQTPVLFDGEFDGRP
                     RKLLAQASRNGWFFVLDRANGQNLLSTEYVKTNWTKGLDARGQPIPDPAKEPQPDGAL
                     VSPNQGGGQNWPPPSFSPRTGLFYANATRAFSVYYLYENENDEKPQG"
BASE COUNT         1429 a         2776 c         2706 g         1240 t
ORIGIN      
        1 gcgcgccgag tgcgtagctg gcggcgatca gaccacggta ggtcatcgga cgctgctcga
       61 tgtcgtcggc gatgacatgg ccgccgccgt gaagccgtcg agcgtcgagc agcgcgtcga
      121 agagcgtcat gtcgatacgc gccgtgtcga atgccgactt caccatctca tcgcgaagag
      181 ctcggcgcag cgcgactcgg cgcgcgcggc cgaccacgcc gtcgggtgac gtcagccggc
      241 gcggcgggag tatccggatg gagatgcgcg ggaacaacct ggttcgaacc ttgccggcca
      301 ggcgtgaaaa cggcgtgagc tcggcgcctt cgatgcggac caggacgagc gcggcgcgcg
      361 tacgctcggc aatgaccgcc gggccttcgt acactttcat cagcgagccc gtcgtggtaa
      421 tccgcccctc ggggaagatg atgcaggccg agccggcctc gactgcacga atcatggagc
      481 gaatcgagag cgggttggtc gggtccaccg gcaccgcatt gaccaacgcc aggaacggcc
      541 gggcccacca cttgcgcgcg atgaaggtat ccaccgcgaa gatcggatcg ccgggcagga
      601 aggcgccgag cagcaggccg tcgagaaacg aggcgtggtt ggcggcgatg acggcatgcg
      661 gcatggcggc gcgcgcgtgc tccagcccgt ccacgcggac ccgatacagc agccgcagca
      721 ccgtgcgcat cacgagcttg gcgaggccgc gacggaccac ccaggcggcg acggcggcca
      781 cgggaatcgt cgccaggccg cccaccgcga agagctcgcc catcgtcagc ccgcgggcca
      841 gcagcaccgc agccgcgacg gccgcgagcg tcatcgccag cgcgttgatg atgttgttcg
      901 ccgcgatgac gcgggcacga tgctgcggct cgctctcgtg ctgcagcagc gcgtagagcg
      961 ggacgcagaa gaggccgccg gcgacggcga ttccgaccag gtcggccaga atccgccagt
     1021 tgcccggcgc gctcacgaag gcggtgaccg acgcgagctc ggacgccggc acgagcctgg
     1081 cgctcgccag gtgcaggtcg atcgcgaaca gccccatcgc gagcgccgca agcgggacgt
     1141 accgggcgct gacctcgccg tgcagcagac gctcggccag catcgagccg atgccgatgc
     1201 cgacggcgaa caacgccagc atcagtgtca cgacgcgctc gttggcgaag agcacgtcct
     1261 tcgcgaggac cggcagcccc gagacgaccg tggcgccgaa cagccagaac caggagacgg
     1321 cgaggatcgg aaggagcagc tcggaacggc ccgtgacgtg ccgcacgacg gcgaccgagt
     1381 cgcgccagat gtgcgggcgc ggagtcgtgg acccgggctc gggcggcgcg ggcgggatct
     1441 ggcgcgaggc gagccatccg cccgcggcgg agacgaggcc gcacgcgccc acgatcaacg
     1501 cgccgttgtc cacgaggacg aggctgcccc ccaggatcgt gccgaacagg atggcgagga
     1561 acgttcccgc ctcgatcagc gcgttgcccg caaccagctc gtcgtcgcgc aggtgctggg
     1621 gaagcagcgc gtatttgatc ggaccgaaca ccgtggagtg cgtgcccaga cagaacagcg
     1681 acgcgagcag cagcgggacg ctctgccggg caagccccag cgccccgagc agcatgatgg
     1741 cgatctcggc aaccttcacc cacctggcga tggcggcctt gtcggtgcgg tccgcgagcg
     1801 tgcccgacgc acccgagaag agaaagaacg gcgcgatgaa cagccctccc gcgatcatga
     1861 cgagcgtggc ggcatccatg ccggtttgcc cggcgatccg gtaggtcacc gcgatgccga
     1921 gcgcgctctt gagaaggttg tcgttgaacg cccccaggaa ctgcgtgatg aagagcggcg
     1981 cgaaccggcg ggccgtcaga agctgcacga gacgcgacgt catgggtttc tcctgtcaga
     2041 cagtggtgac cgcggcccat gccgcggccg tcaaccggtc gcacatcgag ctcatctgcg
     2101 atggtagctg cccgtaggcc gcaaactgca ccggctccag cacgacgcca aagaggaccg
     2161 ccgtggccag gtcgggggac tgagccggga tgtcgcctga cgcgatcgcg cggctcatca
     2221 cggcgcgcac ggcgcgcacc ggcgtcggcg tgtcgggctc gagcttcccg agctggccgt
     2281 gctgcacgaa cagcagaaac cggaacagct cctggttctc gtcgtgcgcg cgacagaagc
     2341 cgtgaatcat cgcgcccacc ttgccccgcg tggtcggctc gctggcggcg agcgcctcga
     2401 gccggccggc gaactcgacg tagtgccgct cgaaggcctg ccagacgagc tcctctttgc
     2461 tggtgaagtg ccggtagaga gcgccttccg agacgcccac ggcacgggcg atatcgcgga
     2521 ccgtggtttc agccacgccc ttgcggacga acaaacggat ggcggccttt tcgatgcggt
     2581 cgcgggtgtc ggtgaatcga tgtctcggca tgtgagtaaa cgattgctca caatattagc
     2641 cgagctgcag actgctgtca atgccagtac ttcaagtctc acttaatcaa tagtttagac
     2701 tcatgcgtag aggcgggtct ttagacccgc ctgatgacag acgtcaagac cgatacgcct
     2761 cgcgtccgaa cccctgcacc ggtatgccct gagacgacag cacctcgtgc tgcagggcgt
     2821 acatgcaggc ggccgcctcg tcgatgcggc ggacgaaggc cagcgagcct tggaggatcg
     2881 gcttcagctt ctcgcgatcc tgaatccgcg ccgccgggta ttcgtgctcc accaccaggt
     2941 gggtgttcgg gatgtcgagc tgcaacagct ctcgcgccgc gagctgatga tcgagccagt
     3001 caaccgtccg gttctgcaga taggcgagcg gatcgtgccg cgcggtcccc ggcagctcgt
     3061 gctcgcacag gaccacctgc ttcttccagg cgttgacctg atcggcgggg ttgtcagacg
     3121 ccttccctcc cacccagtcg ccccgctcca tcgtctgtcc gccgtagccc cacgcggcga
     3181 cgccctttga gtcgatgacc tgtcccttca cgtggaagcc gccaatgagg aagccgtacc
     3241 cggtgtcctt caagtactga aataccgagc gcgtgtcctg ccccatcagg atcgcgtgcg
     3301 agggatcgta gtgaatgcgg aactggtcgc cgacgccgtg cctctcgcag atccggtgca
     3361 gcgcgatcca cgctccgggc gtgtacgcga tgttgttgtg ccagttgtcg ctcgtggtcc
     3421 accccggcat cgggcactgc tcgacgcgat aggtcaagcc tcgcgccttc gccgcctcga
     3481 gcagcgggac gaactgctcc tcgaagtcca cgaggttctg atccatgctg cgctgctggt
     3541 tgcggcccac gaagccgcac accgcgttga cgccaagcag caccgcggca tcgaagaccc
     3601 gcagcaggaa ctcgtgcttc ttcctcctgg tcgccgcgtc gtgatgcagg aggttgtcga
     3661 agtaggcgag gtcggaaaag gcgacgctcg tggacttcac cgccgcttcc acacgccgtg
     3721 ccctggcctt gtcgaacggc tgccgcaagt caagcgtgtt ggccacgggg tcgagcatcg
     3781 cttccggcgg cacgtccgtt tccgacgggt gaagcgccgc cgagagctgg atgtagccgg
     3841 cgccaagctc gcgcgcaaac tgcagccatt cttcgatcgc gaggtctggg tccgcatcgc
     3901 gcgcctcgcg cggcgtcagc tcctggagcg ctgccgtgag gacgccgacc ttcatgaact
     3961 tcggctcgaa cggtctggtc atcgaacctg cgtccacacg ccgcccttgc gggcgctctc
     4021 gagaatcgca tcgacgatgc ggttcgcgcg gtagccgtct tcaaaggtcg cgacggcggg
     4081 cggaagcgcc cggtagggtt tgcccgcggc aatgcatccg tagatgtcga acatcagatt
     4141 ccggaacgca tcggcccatg cttcctggtg cccgccgggg agagatgcat agccgcgtac
     4201 ggcgtcgtca accaacgccg ggtccttctg aagcacctcg ttcgccttgt cgcgatgacc
     4261 gatccacagc tcattctggt gttcctgccg ccaccgcacg gaggccttcg gcccgcacac
     4321 ttcgaggacg aggtcgttct tgtgccctcc acagacctgg cccactgaga aggcgccctt
     4381 cgcgccgttc tcgaatcgga ggagcaccga cgcgagatcc tcgacctcga tgtccacgac
     4441 atcgacggcg cctgctcccc cagcctgaaa ggcctcgcgc gatccgcgcg gtttcttccg
     4501 cttcggcacg acggtcgtga tgtcgccgag cacgtgcgtg atctcgagcc ccgacacgtg
     4561 ttgcgcgaga tcgcaccagt gcgagccgat gtcgccgatg gccgacgagg cgccgctctg
     4621 cttcgggtcg aggcgccagg agtagtcggt gtccttgatc agccagtcct ggaggtaata
     4681 gccgtgcagg aagtgcggga cgccgatgtc gccgcgtgcg acggcgagcc gagcctgctg
     4741 aacgagcggg ttgccgcggt agttgaacgt gacggcggtg acgacgcccg cctgccgcgc
     4801 ctgctcgacc aggcgcttcg attgctcggc cgtcatcgcg agcggcttgt cggcgacgac
     4861 gtgcttgccg cgcgcgagcg cggcggcgat cacctcgtag tgaaggacgt ttggcgtcgc
     4921 gttgtgcacg acctgcacct cggggtcgtc gatcaacgcc tggtagcggc cgtacgcctt
     4981 gcggactccg agggcgtccg ccttcttccg cgccgacgcc tcgttgctgg tggcgatggc
     5041 caccacgtcg acgaagccca ggcgcctgac ggcgtcgata tgatgtggtc cgacgaagcc
     5101 ggcgcccacg aggcccattc ctactcgagt catgcggagg cagattctac cccgcccacg
     5161 tgcctctgct agaatccgcg cgtcaggctc ctgcccccca agcgtggatg agatgaatcg
     5221 ccgagacttc gtcctggcgc ttgctggtcc gctcgcgatc gcacaggtcg gccgtgcgca
     5281 gggcgcggcg cccgcccggt tgaaagggcg aatcaggcag ggcgtcacgc gcggagtgtt
     5341 tgcgcggggc acgccgctcg aggacgcctg ccgcgaagcg gcccggctcg gcatcaccgg
     5401 gttcgacctg atcggccctg ccgactggcc gacgttgagg aagtacggcc tcgtgccctc
     5461 catgtatccg ccggggccgg gcgggacgat ccccgacgcg ctcaaccgca gagagaatca
     5521 cgcgaagctc gaagcctcgc tccatgcggc catcgacgag tgcgcggcca acaaggtccc
     5581 gaacatcatc acgttctccg ggaaccgccg cggcatgtcc gacgccgagg gggccgacaa
     5641 ctgcgtcgcg ttcctgaaca ggatcaaggc gcacgccgag gacaaaggcg tcacgatctc
     5701 gatcgagatc ctcaacagca aggtcaacca ccgcgactac atgttcgacc acatgccgtg
     5761 gggggtcgac gtggtgacgc gcgtgaactc gccgcgcgtc aggatcctct acgacatcta
     5821 ccacgcccag atcatggacg gcgacgtcgt tcgcacgatt cgagatcaca tcgggctcat
     5881 cagtcatttc cacaccggcg gaaacccggg gcgcaacgac atcgacgaga cccaggagct
     5941 caactaccgc tacgtcatgc aggagatcgc cgctctcggc ttcacggggt tcgtcacgca
     6001 cgaatacacg ccgaaggcag gcagcgatcc gatcgcggtg ctccgcaagg cggtcgagat
     6061 ttgtgatgtc taggaccagc gcggcgttcg cgatcgtgct cttggtcgca acgggttcgc
     6121 tcgcggcgca aggtggaccg taccaacggc agcggatcga caccgccgcg gccgaccgcg
     6181 ggcgcgccgt gtacgcgcgg ctctgcatca actgccatgg cagcacggcc aaggggaccg
     6241 agcgcggacc cgacctgatt cgatcggcca ccgtgctgcg cgatcgcctc ggaaacctga
     6301 ttggacccgc actgaggacg gccacgcacc ggtccggggt cacggagacg caagtcgtag
     6361 atctgtcgca cttcctccat gagcgcgtcg aggcgacggc caggaatcgc acgccgacgg
     6421 cgccgctgaa cgtcctgaca ggcgaccccg aggccgggcg cgagtacttc aacggcgccg
     6481 ggacgtgcag cgcctgccac tcctccaccg gtgacctggc cggcatcgcc acacggatca
     6541 ccgatccggt caacctgcag caacgcttcc tgttcccgac gctgcgccgc gacggggcaa
     6601 ggcgcgtcga ggtcaccgtg gcgcccgcat ccgggccggc ggtctcggga gcgctggtgc
     6661 gcatggacga cttctccgtc tcgctgcggg acgcctccgg cgagtatcac tccttctccc
     6721 gggtgccggg cacgacagtg tcggtccgca acccgcttgc cgctcatcac gaactgctcg
     6781 atcgctacac ggacacacac atgcacgaca tcactgccta cctctcgaca ttgaaatgaa
     6841 gacgatccac cttcgcgcgg ggcgctacgg cggacacgtc atcacgctcg cggccgccgt
     6901 gctcgctgct gccgcgctcg gcgcgcaggg aacgctcgct cccgccgaac tgcttcggcc
     6961 gctgggcgcc gcgtggccga tgtacaacgg cgactactcc ggacagcggt tcagcgcgct
     7021 ctcgagtatc gacacgtcga acgtgtcgtc actgacgctc gcctgggtgc atcgaccgaa
     7081 cgccggcgcg gcgcccgccg gcggaggcgg cgcgacagcc gtcacgatca agggcactcc
     7141 ggtcgtcgtc aacggcgtgc tctacgtcac gatccccgat cacgtctggg cgctggacgc
     7201 gcgcaccggg cgagagatct ggcatgcgac gtggccgtcg aaaggcgggt ggcacatcgc
     7261 caaccgcggc gtcgccgtgc tcgggagcac gctctacgtc gaaacgcccg actgcaacct
     7321 cgtggcgctc gacgtccgcg acggcaagga gaagtggcgc acggaaatct gcgatctcga
     7381 gcagttctac tacgcctcca cggcccccct cgtcgtcggc aggcacgtga ttgtcggcgt
     7441 cagcggagac gatctcgaca tccccggcta cctccaggcg cacgatcccg acaccggcgc
     7501 acggcagtgg cggtggtaca cgtaccctga gccgggaacg ccggaagcca agacgtggcc
     7561 cagtgtcgaa gcgatgatgc acggcggcgg aatgacctgg ggatcgacca cctatgatcc
     7621 agaactcaat ctgatctact tcgggacagg caatccgcag ccggtgatca acggccgcaa
     7681 gcgccagggc gacaacctct acacggagtc gatcgtcgcg ctcaaccccg acaccggaaa
     7741 gctggcctgg tacttccagt cgtcgccgca cgacacccac gactgggacg cgacgcagac
     7801 gccggtgctg ttcgacggcg agttcgacgg acggccgcgg aagcttctcg cccaggcgag
     7861 ccgcaacggg tggttcttcg tcctcgatcg cgccaacggg cagaacctcc tgagcaccga
     7921 gtacgtgaag acgaactgga cgaaggggct cgacgcgcgg ggacagccga ttccggaccc
     7981 ggcgaaagag cctcagcccg acggcgctct tgtgtcgccc aatcaagggg gcgggcagaa
     8041 ctggccgccg ccgagcttca gcccccggac ggggctcttc tacgcgaacg cgacgcgcgc
     8101 cttcagcgtg tactacctgt acgaaaacga gaacgacgag aagccgcagg g
//
LOCUS       DBNGSH010000258         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1208359, whole genome shotgun sequence.
ACCESSION   DBNGSH010000258 DBNGSH010000000
VERSION     DBNGSH010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1208359"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="AC16CY_05920"
     CDS             complement(<1..496)
                     /locus_tag="AC16CY_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HIR3353354.1"
                     /translation="MSEPWKTDKWFSSPWSYLPEVRQKNTFGKNLKIHDVTLRDGEQQ
                     TAVVFRREEKVAIARKLDSLGIHRIEAGMPAVSAQDKAAISDIAALGLKAEIFGFARC
                     IPAEIDVIKACGCSGVVIEIPASDHMIKYGYGWPLERAMKSSIETTLAAKAAGLYTVF
                     FTIDA"
     gene            646..>2665
                     /locus_tag="AC16CY_05925"
     CDS             646..>2665
                     /locus_tag="AC16CY_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3353355.1"
                     /translation="MHLLEVLREECGIVSAKNGCAPEGACGCCLVLVDGQPALSCLRR
                     PEQTEGREVVTLEGIPEETRRLMADAFVLEGGVQCGFCIPGIVVRAATLVERGKTGDR
                     AAIAKALDGHLCRCTGYGRIIDAIQTAGEAAANGGSLTRTEPRRNSFFGEEFGLKRNP
                     AFAKGNGNGHGAGHGIGRSIARQDGSAQTLGDKPFIDDMRVPGMLHGAMVLTEHPRAR
                     ILKIRTDDAAAMPGVVRVLTAADVPGFRGTGLNDPDQPVFVAEGELTCCVADFLAMVV
                     ADTQFHARQAAKAVKIDYEVLEPLTDPFEALKPGATLVHSADTFRPTPSNVLQPITAF
                     SRGDVDAALAGAAHVIEATFQTQPVEIAFLEPEACLVEPEGKGVKVYTDSQGSVYDHQ
                     QIAKVLKLDPKDVEIVLLPSGGAFGAKEELSIQAQTALAALLLGRPVKTVLTREQSTQ
                     HHVKRHAMTVKLTVGADADGRLLALRSRIVADAGGYHTTSAKCALRAACHSTGVYRIP
                     NVDVESKAVYTNNPNGGAMRGFGSNQAQFAMEGMMDMLAERVGVDGWDIRERNILDPG
                     DKFGTGQVMRESVRGIRATLDAVRDVYKRSRYKGIGCGIKSTGLGNGTIEGGHIVIRV
                     LGGPRLEIRNGYTEMGQGVYTATMQAVCEESGLPADIMTVVWDKDLGEK"
BASE COUNT          471 a          861 c          913 g          420 t
ORIGIN      
        1 tggcgtcgat cgtgaagaag acggtgtaga ggccggcggc ctttgcggcc agcgtggtct
       61 cgatcgagga cttcatcgcg cgctcgagcg gccagccgta gccgtacttg atcatgtggt
      121 cgctggccgg aatctcgatc acgacgcccg aacagccgca cgccttgatg acgtcgatct
      181 cggccgggat gcagcgcgcg aagccgaaga tctcggcctt gagccccagc gccgcgatgt
      241 cggagatcgc cgccttgtcc tgggccgaca cggcgggcat gccggcctcg atgcggtgga
      301 tgcccagaga gtcgagcttc cgcgcgatcg ctactttctc ttcacggcgg aacacgacgg
      361 ccgtctgctg ctcgccgtcg cgcagcgtga cgtcgtgaat cttgaggttt ttgccgaacg
      421 tgttcttctg ccgcacctcg gggaggtacg accacgggct gctgaaccac ttgtctgtct
      481 tccaaggctc gctcatctca actcccgatt cccaacgccc aattcccaac ttcggaccca
      541 gcgcagccga tgatgttacg atccccttca gggcaaagtc cacgaatgcc gcgtatctgc
      601 ttcactttga acgggaaacg gaccgaggcg tcctacgagc ccgggatgca tctgctcgaa
      661 gtccttcgcg aggaatgcgg catcgtctcg gcgaagaacg ggtgcgcgcc cgagggggcg
      721 tgcgggtgct gcctcgtgct ggtcgatggc cagcccgcgc tttcctgcct gcgccggccg
      781 gagcagaccg agggccgcga ggtcgtcacg ctcgaaggga tcccggaaga gacccggcgg
      841 ctcatggcgg acgccttcgt cctcgaaggt ggcgtccagt gtggtttctg catccctggc
      901 atcgtcgtcc gggccgcgac gctcgtggag cgcggaaaga cgggcgaccg cgcggcaatc
      961 gccaaggcgc tcgacgggca cctgtgtcgc tgcaccgggt acggacgcat catcgacgcg
     1021 attcagacgg ctggagaggc ggcagccaac ggcggcagtc tgacgcgcac cgagccgcgg
     1081 cggaattcgt ttttcggcga ggagttcgga ctgaaacgga atccggcctt tgccaagggc
     1141 aacggcaacg gacacggagc aggccacggg atcggacgat cgatcgcccg ccaggacggg
     1201 tcggcccaga cgctcggcga caagccgttc atcgacgaca tgcgagtgcc cggcatgctt
     1261 cacggcgcga tggtgctgac cgagcacccg cgcgcgagga tcctgaagat tcgcaccgac
     1321 gacgcggccg ccatgcctgg cgttgtgcgg gtactgactg cggccgacgt gcccggcttc
     1381 cgtggaacag gcctgaacga tccggaccag cccgtcttcg tggccgaggg cgagctcacg
     1441 tgctgcgtgg cggacttcct ggcgatggtg gtggccgaca cgcagttcca cgcccgtcag
     1501 gcagccaagg ccgtgaagat cgattacgag gtcctcgagc cgctgaccga tccgttcgag
     1561 gcgctgaagc cgggcgcgac gctcgtccat tccgccgaca cgttccgtcc gaccccgtcg
     1621 aacgtcctcc agccaatcac ggcgttctcg cgcggcgacg ttgacgcggc gctggccggc
     1681 gcggctcacg tgatcgaggc caccttccag acacagccgg tggagattgc gttcctcgag
     1741 ccggaggcgt gccttgtcga gccggaggga aaaggcgtca aggtctatac cgacagccaa
     1801 ggctcggtat atgaccacca gcagattgcc aaggttctga agctcgatcc gaaggacgtc
     1861 gagatcgtcc tgctcccgag cggcggggcc ttcggcgcca aggaggagct gtcgatccag
     1921 gcgcagacgg cgctcgccgc actgctcctt ggccggccgg tcaagaccgt gctcacgcgc
     1981 gagcagtcga cgcagcacca cgtcaagcgt cacgcgatga cggtgaagct cacggtcggc
     2041 gccgatgccg acgggcgcct gctcgcgctg cggtcgcgaa tcgtcgccga cgcaggcggc
     2101 taccacacga caagcgccaa gtgcgcgctc cgggcggcct gccactcgac cggcgtctac
     2161 cgcataccca acgtggacgt cgagtcgaag gcggtctaca cgaacaaccc gaatggcggc
     2221 gcgatgcgcg gcttcggcag caaccaggcg cagttcgcga tggaaggcat gatggacatg
     2281 ctcgccgagc gcgtgggcgt ggacggctgg gacatccgtg agcggaacat cctcgatccg
     2341 ggagacaagt tcggaaccgg acaagtgatg cgcgagagcg tgcgcggcat ccgcgccacg
     2401 ctcgacgcgg tcagggacgt ctacaagcgg tcgcgctaca agggcatcgg ctgcggcatc
     2461 aagagcaccg ggctgggcaa cggaacgatc gagggaggac acatcgtcat ccgcgtgctc
     2521 ggggggccgc ggctcgagat ccgcaacggc tacacggaga tgggccaggg cgtctatacg
     2581 gccacgatgc aggcggtgtg cgaggagtcg ggccttcccg ccgacatcat gacggtcgtg
     2641 tgggacaagg atctcggcga gaagt
//
LOCUS       DBNGSH010000259         2091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4434810, whole genome shotgun sequence.
ACCESSION   DBNGSH010000259 DBNGSH010000000
VERSION     DBNGSH010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4434810"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /gene="cutA"
                     /locus_tag="AC16CY_05930"
     CDS             <1..326
                     /gene="cutA"
                     /locus_tag="AC16CY_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017871922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR3353356.1"
                     /translation="TASSPVSVVLTTIAAREDAIALARTLIEERLAACVNVLPQMTSV
                     YRWKGAIEEEPEHQVVIKTASDRLPALEARLRQLHPYEVPEFVVLEAGDHENPYLTWV
                     RESVG"
     gene            complement(350..967)
                     /locus_tag="AC16CY_05935"
     CDS             complement(350..967)
                     /locus_tag="AC16CY_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR3353357.1"
                     /translation="MGFVEQFFSTVYNVPELIRLVGFYGLILIVFAETGLLVGFFLPG
                     DSLLITAGLFAARGDFDIVTLNLVLCAAAILGDATGYWIGRRTGIALYSRPDSFFFRR
                     EHLRMTHDYYERHGGKTIVIARFVPILRTFAPVVAGVAEMKYRTFATYNIVGGIAWVL
                     SMTLGGYALGNLIPDIEKRIHIVVAVVIGLSLLPAVFAWLRSRQK"
     gene            complement(967..2091)
                     /locus_tag="AC16CY_05940"
     CDS             complement(967..2091)
                     /locus_tag="AC16CY_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR3353358.1"
                     /translation="MCIHAGQEPDPATGAIVTPIYQTSTYVQEALGRHKGYEYARTQN
                     PTRSALEANIAAIEGGKAAFAFASGMAATGAVMTLLQSGDHVVVTDNTYGGTYRLFER
                     VLRKYQLDFTYVDTSNLDEIRRAIRPTTKMLFIETPTNPLLRLTDLAGACQIARERGV
                     VSVVDNTFASPAVQRPIEFGADLVVHSTTKFLNGHSDSVGGIVVAVRDEHVEWLKFVQ
                     NAEGAILGPMDAWLVLRGTKTLPLRMDRHNASAQMLAEYLAAHPKVSRVHYPGLPDHP
                     QHALARRQMKGFGGLIAFELGSLDRARTLLNGVRLMSLAESLGGVETLISHPATMTHA
                     SVPPERRRQIGLTDDMVRISVGIEDVDDLKEDLAQALERV"
BASE COUNT          351 a          688 c          701 g          351 t
ORIGIN      
        1 tgactgcatc aagcccggtg tccgtcgtcc tgaccacaat cgccgcccga gaggacgcca
       61 tcgccctcgc cagaacgctc atcgaggagc gcctggccgc ctgcgtgaac gtcctgccgc
      121 agatgacgtc ggtctaccgg tggaaagggg cgattgagga agagccagag catcaggtgg
      181 tgatcaaaac ggcgtccgat cggctgcccg cactggaggc gcggctgagg cagctgcatc
      241 cctacgaggt tcctgagttc gtggtgctgg aggccggcga tcacgagaac ccgtacctca
      301 cgtgggttcg cgagtcggtt ggttaggcgg gtctaaagac ccgcctctac tacttttgtc
      361 tactcctcag ccaggcgaag accgccggca gcagcgacag gccgatgacg accgccacga
      421 cgatgtggat ccgtttctcg atgtcgggga tcaggttgcc aagcgcgtag cccccgaggg
      481 tcatgctcag gacccaggcg atcccgccga cgatgttgta ggtggcaaac gtccggtact
      541 tcatctccgc cacgcccgcc acgaccggcg cgaacgtccg gaggatcggc acgaacctgg
      601 cgatgacgat cgtcttcccg ccgtggcgct cgtagtagtc gtgcgtcatc cgcaggtgct
      661 cgcgccggaa gaagaacgag tccggccggc tgtacagcgc gatgcccgtg cggcggccga
      721 tccagtagcc ggtggcgtcg ccgaggatgg ctgcggcgca cagcaccagg ttcagcgtca
      781 cgatgtcgaa gtcgccgcgc gccgcgaaga ggcccgccgt gatcagcagc gaatcgccgg
      841 gcaggaagaa gccgacgagc agtcccgtct cggcaaagac gatcaggatc agtccgtaga
      901 agccgacgag ccgaatgagc tccgggacgt tgtagacggt ggagaagaac tgctcgacga
      961 accccatcaa acacgctcga gcgcctgcgc caggtcttcc ttcagatcgt cgacgtcttc
     1021 gatgccgacc gaaatccgca ccatgtcgtc ggtcagtccg atctgcctgc gccgctcggg
     1081 aggcaccgac gcgtgggtca tcgtcgcggg gtgtgagatg agcgtttcga cgccgccgag
     1141 gctttcggcc agcgacatca gcctgacgcc gttcagcagg gtccgcgccc tgtccagcga
     1201 cccgagctcg aaggcgatca ggccgccgaa ccccttcatc tggcggcgcg ccagcgcgtg
     1261 ctgcggatga tcgggcaggc cgggatagtg gacccgcgac accttcggat gcgccgcgag
     1321 gtattcggcc agcatctgcg cgctcgcgtt gtggcgatcc atgcgcagcg gaagcgtctt
     1381 ggtgccgcgc agcacgagcc acgcgtccat cggtcccagg atcgcgccct cggcgttctg
     1441 cacgaacttc agccactcga cgtgctcatc gcgcaccgcg acgacgatgc ccccgacgct
     1501 gtcgctgtgg ccgttcagga acttcgtcgt gctgtggacg accaggtcgg cgccgaactc
     1561 gatgggccgc tgcacggccg ggctcgcgaa cgtgttgtcc accaccgaga ccacgccgcg
     1621 ctcgcgcgcg atctggcacg ctccggccag gtcggtcagc cgcaggaggg gattggtcgg
     1681 cgtctcgatg aagagcatct tcgtcgtggg ccggatcgcg cgcctgatct cgtcgaggtt
     1741 cgacgtgtca acgtaggtga agtccagctg gtacttgcgc aggacgcgtt cgaacaggcg
     1801 gtaggtgccg ccatacgtgt tgtcggtcac gaccacgtgg tcacccgact gcagcagcgt
     1861 catcacggcg cccgtggccg ccatccccga tgcgaaggca aaggccgcct tgccgccctc
     1921 gatggcggcg atgttcgcct cgagcgcgct gcgcgtcggg ttctgcgtcc tcgcgtactc
     1981 gtagcccttg tgccggccga gcgcttcctg cacgtaggtt gacgtctggt agatcggcgt
     2041 cacgatggcg ccggtcgcag ggtcgggctc ctggccggcg tggatgcaca c
//
LOCUS       DBNGSH010000260        13691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1208481, whole genome shotgun sequence.
ACCESSION   DBNGSH010000260 DBNGSH010000000
VERSION     DBNGSH010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1208481"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="AC16CY_05945"
     CDS             complement(<1..779)
                     /locus_tag="AC16CY_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353359.1"
                     /translation="MSDIDRFRPDDRRGVDTLYRRMHGTDAAEAVRLRWDWQHRRNPN
                     NSTGLPGIWVAREGPTVVGHYPTLPVRLSLKGMEIEGAWGTGALIAPERDRQGLDEAL
                     VRAWDRNSGAVLAFGASAESRGIIDRLHWPPSHVVPSLVKPLTRRALRFPHLPMPFNR
                     VISALVYPVVRVVARSRPLRAECEPIRRFDNSFTALWERLAPKFDLAVRRDSPYLNWR
                     YVEPPHVRYSIVALKRQGEVHGYAVYRHRHEPLGRVTMLVD"
     gene            complement(825..1499)
                     /locus_tag="AC16CY_05950"
     CDS             complement(825..1499)
                     /locus_tag="AC16CY_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353360.1"
                     /translation="MSIRGVAAALIVLLCGAAPAPAQTVSLEFQNGRVRLVAENASVG
                     QILAEWSRLGGTKIVNGERIPGAPLTLQLEDVSERQALDVVLRGAAGYMVAGREVAAA
                     RASVFDRILILPTSTRAPNTASLPPQPQSAPAMIIDDVDDDVVPRPGQPGQQPPAVIR
                     LPQGRVLPGIEGQNPPDEVPAQDPTMPRSPVRANPFGVTSGAARPGTISPVTRPRNPN
                     EPPQER"
     gene            complement(1544..2509)
                     /locus_tag="AC16CY_05955"
     CDS             complement(1544..2509)
                     /locus_tag="AC16CY_05955"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3353361.1"
                     /translation="MKALLTGGAGFIGSHLAERLLQLGDEVLVIDDLSTGSIENISHL
                     KSTRGFSYTIDSINNESLLAELIDRSDVVFHLAAAVGVKLIVEQPVRTIETNVHGTEV
                     VLKHASKKRKLVVLASTSEVYGKSTDVPFREGAELVLGATVKHRWAYACSKLLDEFLA
                     LAYWKERKLPVVIARLFNTVGPRQTGQYGMVLPTFVGQAIAGRPLTVFGDGRQSRSFT
                     YVGDVVDALIGLAREPRAVGEVFNIGSKNEVTIRALAERVKQLADSDSVIQYVPYDQA
                     YEAGFEDMPRRVPDLSKIAALIGYEPKLGLDEIIRSVVEYARNNV"
     gene            complement(2506..3555)
                     /locus_tag="AC16CY_05960"
     CDS             complement(2506..3555)
                     /locus_tag="AC16CY_05960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3353362.1"
                     /translation="MRAAIARVVDSGWFVLGPEVEAFETEFASACGAPAAVGVGNGTD
                     AITLALRALGVGPGDEVIIPAMTAAFTGLAVVAAGARPRIVDVEPDALTLDASACAAA
                     VNSRTKAIVPVHLYGQPADMDAIRRVADRHALAIVEDCCQAHLATGRGVPVGTRSHAG
                     AFSFYPTKNLGALGDGGAVITGDRALADRVRRLRNGGQRDRYHHEDPGVNSRLDELQA
                     AVLRARLPLLPGWTARRRELAALYRRELPGGLQPVPEREAGHVYHLFPVRAPARDALA
                     AHLATAGIGTLVHYPVPLSAQRAFAAFEPDECPVAARAAAELLSLPLHPRLEAADVSR
                     VSREVTMYWKGRTQA"
     gene            complement(3612..4640)
                     /locus_tag="AC16CY_05965"
     CDS             complement(3612..4640)
                     /locus_tag="AC16CY_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3353363.1"
                     /translation="MTDQDYLAFYQGRQVLITGGLGFIGSNLARQLVALGARVLVIDS
                     LISDYGGNLFNIDGLENRLRVNIADIRQQSTMNYLVRGHDVIFNLAGQVSHIDSMQDP
                     YTDLEINCRAQLSLLEACRHNNPTVKVVFAGTRQIYGRADALPVTEAHLVRPTDVNGI
                     NKAAGEYYHMMYNNVFGVRTVSLRLTNVYGPRQLIKHNRQGFIGWFIRQAIENREIQI
                     YGDGGQLRDFVFVDDAADAFLRAGASDACNGQVFNVGGQEAITLRELVELLISVAGTG
                     RYRLIAWPPEKKAIDIGDFHADSSLIARTLGWRPTTPLRDGLRRTIEFYRAHLPHYVP
                     TGSPVAAL"
     gene            complement(4637..5449)
                     /locus_tag="AC16CY_05970"
     CDS             complement(4637..5449)
                     /locus_tag="AC16CY_05970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3353364.1"
                     /translation="MPERPATRAPGLTMFFPAYNDAGTIASLVIQAQEAIRHLTPDFE
                     VLVINDGSRDQTAQIADELARTYPNVRVIHHVSNRGYGGALRTGFASATKELIAYTDG
                     DAQYDPSEIATLWARLGPDADVVTGYKISRSDPLHRILIGRIYHYTVKLLFALRVRDV
                     DCDFRLMRREVFDRVKLDRDSGVICLELMRKIQDAGLRVVEVPVHHYHRSYGRSQFFN
                     FRRVFRTGIDVLKLWIELVVLGRGRQSTPVLFSNPAVRRPSPPGPTTPGARP"
     gene            complement(5545..6633)
                     /locus_tag="AC16CY_05975"
     CDS             complement(5545..6633)
                     /locus_tag="AC16CY_05975"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR3353365.1"
                     /translation="MLRAALIGVPSSGKTTLFQLMTSARDAPRGKGDVNIGISKVPDA
                     RLDVLTAMYNPRKRVPATVEFTDVVAPARTGVQALVDLAGYKNADALVHVLRAFRDDA
                     VAHPSGSIDPARDAQAMEDELILADLGVAERRLERIEKDLKKSRSAEAEKERDLVRRC
                     TAALEEGRPLRALDISGDDLKRLRGFQFLSAKPLLLVINLDESEAAAVGSDLGKAAAG
                     TGLTGFLARASTGAVALCAKIELEIAQLEGPDAQAFLSDLGLSESGLDRVIRSSYDLL
                     GYMSFFTVGEDECRAWSIARGTAAQAAAGEIHTDIQRGFIRAEVVAYEALVARGSMAA
                     CREHGEVRLEGKEYVVRDGDVINFRFAT"
     gene            6926..7306
                     /locus_tag="AC16CY_05980"
     CDS             6926..7306
                     /locus_tag="AC16CY_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR3353366.1"
                     /translation="MFRAVIQDQQGADVFVTSLSGESVRIYPLAVWIEIERKLMEMPG
                     NHPSRLKFLDRVNYYGQASELDGQGRVVIPQHLRESASIVGDVRVFGRISYLEVWNDQ
                     RFLQKLQREAWTDEDGLRLSEHGV"
     gene            7331..8239
                     /gene="rsmH"
                     /locus_tag="AC16CY_05985"
     CDS             7331..8239
                     /gene="rsmH"
                     /locus_tag="AC16CY_05985"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR3353367.1"
                     /translation="MVEHVPVMVQEVLEHLEPSRGGLFIDCTVGLGGHARAILDAGAS
                     RVIGLDRDPQALERARETLGEVGDRVELVHADYRRFEDVLNARGVGPVAGILADLGVS
                     SLQLDSPGRGFSFRRDEPLDMRMDTSSGATAADLIASADEQTLADVLYTLGEERYARR
                     VARAILGESKERRIDTTGQLAAIVRRAIPRKGFSRIDPATRTFQAIRIWVNQELEGLD
                     AFLVRAAGRLARGGRLVVVTFHSLEDRVVKHTLRALESSATLAVRTRRPMVPSEAEIG
                     RNPRARSAKLRAAERQATGDGGGHDV"
     gene            8229..8621
                     /locus_tag="AC16CY_05990"
     CDS             8229..8621
                     /locus_tag="AC16CY_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR3353368.1"
                     /translation="MTFESFEYSIKKDIRNNPIIREVDRERHREMWRSMFIGVFLVAV
                     LLFSAWQHFELQQHGRRHEQMLQERADAEFVNLHLRLEIETLKAPARIEGFATRRLRM
                     VAPAAEDSTVIERVTAAPAPPTSVVAQR"
     gene            8641..10656
                     /locus_tag="AC16CY_05995"
     CDS             8641..10656
                     /locus_tag="AC16CY_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HIR3353369.1"
                     /translation="MADKPSIEWRITLKRRLTIAVCLLFAWAGAIEARLIYLQVFRHA
                     DFAARAEQQQSRRIPSPAKRGELLDRHGKILARSVDADSVYADPSEVGDPDRAAARLC
                     EALRDCDADDRRGIADRIRKGRYFAHVQRQVTHEQARRVAELKLEGVGFIKESRRYYP
                     NRDLASQLLGYVGIDNVGLSGIEKAYDRIILGKPGTVVVQTDGNRQAFSREERPPTTG
                     ASIELTIDQYIQHVAERELEAGVKSSGASTGSAVVMDPHTGELFALASYPGFNLNTYR
                     EARPDARRNRAVQDYYEPGSTFKIVTASAALEEKAISPEELIDASAGNIRFGSRVIND
                     DHNYGVLSFSDVIVKSSNVGAIKVALKLGPRKMGDYVKRFGFGRPSSPDFRGESPGMV
                     WDPSSLNDSALASVAMGYQVGVTALQMAAAVSVVANGGELVQPRAVRAVIHDGKRLPV
                     PHKTLERVISRGTAVTMAEIMEKVVEEGTGTRARIPGYTVAGKTGTAKKLVRGSYRGH
                     SDYNVSFVGFVPSRAPVFAIVVVVDAPHRVSPYGGVVAAPIFQKIADAALRHYGVSPS
                     INAPEPVLAGRWEERLELTAGPAAPRAIVPLGGSSGEVFPDLTGLSAREATAILTRLG
                     YTVRLRGDAGLVVEQTPEPGTPLESSTTAVLTVERRAADLESSAGVP"
     gene            10653..12158
                     /locus_tag="AC16CY_06000"
     CDS             10653..12158
                     /locus_tag="AC16CY_06000"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR3353370.1"
                     /translation="MTVGDVVRAVAREGERQLAGSSSGLDRPCTGVTHDSRRVEPGTV
                     FVALRGLKADGAAFVPQAIAAGAAAIVAERGPEGSVGVPWVVVPDARLALALASAEFF
                     GHPSRQMQVVGITGTNGKTTTAYLVRAILETAGVKSGLMGTVTYQLGDRSFDATRTTP
                     EAPDVQGFMREMVASGCRACAMEVSSHALALRRVDGIRFAAAVFTNLTRDHLDFHSDM
                     DDYFAAKRRLFDMLPEGAPAVINADDPRGASLVDSVRRPVTYGMTRTADVSPGPIAYS
                     LDGLQFDIRAPKGAVRVRSRLVGKPNAYNILAAVATATELGVPTEAVEAGLERLEGVP
                     GRFQMASGASDDVRAVVDYAHTDDALRNLLETARPMATRRLITVFGCGGDRDRTKRPL
                     MGMVAARLSDVVVITSDNPRSEDPNQIIEEIRRGADAETRQSRAEVLIRPDRRDAIRD
                     AVELAGPGDLVLVVGKGHEKYQEIGGRTFAFDDVAVAKEALEARRLKSRAG"
     gene            12155..13546
                     /locus_tag="AC16CY_06005"
     CDS             12155..13546
                     /locus_tag="AC16CY_06005"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR3353371.1"
                     /translation="MTGVSITLDWVASAVGATSRAGAGGAVVGDVVTDSRTLKPGDLF
                     VALRGPRFDGHAFAAEVLERGAAGVIVERGRAAGDAHAIEVEDTLAALQGLAHAVRKA
                     VGTRVIAITGSAGKTTTKEAIAAVLATRYRVVKNKGNLNNHIGLPLSLMQLRTAPDVA
                     VMELGMNHAGEISRLVAIAEPEVRVWTNVGDAHLGFFESADAIADAKAEILEGATADA
                     VLVSNADDARVAARAGRFPGRTITFGESPGATVRALSTRLHGVQGVSADVATPAGTRS
                     LATPLLGRGNMWNVLAATAVGLEFGLDLDAIAAEVARLQPADRRGVVRHLRDGITLVD
                     DSYNSSPSALARALDVVAYEAHARRAVGVLGEMLELGEHARVLHEASGRAAAAAGLRR
                     LFVVGGAAARAMADAAVAEGMPASAVSYFERSTEAAPAVVAAVEAGDLVLVKGSRGTR
                     TDVIADQLVTELG"
BASE COUNT         2178 a         4470 c         4817 g         2226 t
ORIGIN      
        1 aagtccacca gcatcgtcac gcgcccgagc ggttcgtgcc ggtggcggta caccgcgtaa
       61 ccgtgaacct cgccctgccg cttgagcgcc acgatcgagt accggacgtg cggcggctcg
      121 acgtagcgcc agttcaggta gggcgaatcg cggcggacag ccaggtcgaa tttcggcgcg
      181 agccgctccc acagcgccgt gaacgagttg tcgaagcgcc ggatgggctc gcactcggcc
      241 cgaagcgggc gcgagcgcgc gacgacccgc acgaccggat acaccagcgc ggagatgacg
      301 cggttgaacg gcatcggcag gtggggaaac cgcagcgcgc gccgcgtgag aggcttcacg
      361 aggctcggca ccacgtgcga gggcggccag tgcaggcgat cgatgatgcc ccgcgattcg
      421 gcggacgcgc cgaaggcgag cacggcgccg ctgttgcgat cccacgcgcg caccagcgct
      481 tcgtccagcc cttgccgatc gcgttcggga gcaatcaagg cgccggttcc ccaggcgcct
      541 tcgatctcca tccccttgag cgatagccgg accggaagcg tggggtagtg tccgacgacg
      601 gtcggcccct cgcgcgcgac ccagattcca ggcagccccg tcgagttgtt cgggttgcgc
      661 cggtgctgcc aatcccaccg gaggcggacg gcttcggcgg cgtcggtgcc gtgcatgcgt
      721 cgatacagcg tgtcgacgcc gcggcgatcg tcggggcgaa agcgatcgat gtcagacata
      781 ccggcgcaag gagtctgcgc ctattctacg ggacagcgcg acggctaccg ctcttgcggg
      841 ggttcgttgg gattgcgtgg gcgagtcacc ggagaaatgg ttccgggccg cgccgcgccc
      901 gaggtcacgc cgaacgggtt ggcccgcacg ggagacctcg gcatggtcgg atcctgcgcc
      961 ggcacttcgt cgggaggatt ctggccttcg attcccggca ggacgcgacc ctgaggcagg
     1021 cggatcacgg cggggggttg ctgtccgggt tgtccgggcc gtggcaccac gtcgtcatcc
     1081 acatcgtcaa tgatcatcgc gggcgcggat tgcggttgcg gcggcaggga cgccgtattc
     1141 ggcgcacgcg tcgaggtcgg aaggatgaga atcctgtcga agacggacgc acgcgccgcg
     1201 gccacctcgc gcccggccac catgtatccg gcagccccgc gaagcaccac gtcgagcgcc
     1261 tgccgttccg acacgtcctc gagctgaagc gtcaggggcg ccccggggat gcgttcgccg
     1321 ttgacgatct tcgtgccccc gagccgcgac cattccgcca ggatctggcc taccgatgcg
     1381 ttctccgcga ccaggcgcac ccgcccgttc tggaactcca gcgacaccgt ttgcgccggg
     1441 gccggagcgg caccgcacag gaggacgatc agggccgccg cgacgcctcg tatgctcatt
     1501 ccagtcagta taagcccgcc gaggggacct ggctatacac gcgctacacg ttatttcgcg
     1561 cgtactccac gacgctgcga atgatctcgt cgaggccgag cttcggctcg tacccgatga
     1621 gcgcggcaat cttgctgagg tcgggcaccc gtctcggcat gtcctcgaac ccggcctcgt
     1681 aggcctggtc gtagggcacg tactgaatca ccgaatcgct gtcggcaagc tgcttgacgc
     1741 gctcggccag tgcccgaatc gtcacctcgt tcttgctgcc gatgttgaac acctcgccca
     1801 cggcgcgggg ctcccgggca agcccgatga gcgcgtccac gacgtctccc acgtaggtaa
     1861 agctgcgcga ctgcctgccg tcgccgaaga cggtgagggg acggccggcg atcgcctgcc
     1921 cgacgaaggt cggaagcacc atcccgtact gtccggtctg ccgcggcccc acggtgttga
     1981 acagtcgagc gatcacgacc gggagctttc gctccttcca gtaggcaagc gcgagaaact
     2041 cgtcgagcag cttgctgcag gcgtacgccc acctgtgctt gacggtcgcg ccgagcacga
     2101 gctcggcgcc ctcgcggaag gggacatcgg tgctcttgcc gtacacctcc gacgtcgacg
     2161 ccaggacaac gagctttctc ttcttgctgg cgtgcttcag caccacctcg gtgccgtgca
     2221 cgttggtctc gatcgttcgc accggctgct cgacgatcag cttcactccc acggcggcgg
     2281 ccagatggaa gacgacgtcg ctgcggtcga tcagttccgc cagcagcgac tcgttgttga
     2341 tcgagtcgat ggtgtaggaa aagccgcgcg tgctcttgag atgagagatg ttctcgatgg
     2401 atcccgtcga caggtcgtcg atcaccagga cctcgtcgcc gagctgcagc aggcgttcgg
     2461 cgaggtgcga gccgatgaac cccgcgccgc cggtgagcag tgccttcacg cttgtgtgcg
     2521 tcctttccag tacatcgtca cttcgcggct cacgcgagac acgtccgcgg cctcgagccg
     2581 cgggtggagc gggagcgaga gaagctccgc ggccgcgcgg gcggccacgg ggcactcgtc
     2641 cggttcgaac gccgcgaagg cgcgctgcgc tgacagcgga acgggatagt gaacgagcgt
     2701 cccgatgcct gccgttgcca gatgagcggc aagcgcgtcc ctcgcgggag cgcgcacggg
     2761 gaacagatgg tacacgtgac cggcctcgcg ttcgggtacc ggctggaggc cgccgggcag
     2821 ttcgcggcga tagagcgccg ccagctcgcg gcgacgcgcc gtccagcccg gcagcagcgg
     2881 cagccgtgcg cggagcacgg ccgcctgcag ttcatccagg cggctgttca cgccgggatc
     2941 ctcgtggtga tagcggtcgc gctggccgcc gttccgcagg cggcgcacgc gatcggccag
     3001 cgcgcggtcg cccgtgatga ccgcaccgcc gtctccgagc gcaccgagat tcttggtcgg
     3061 atagaagctg aacgccccgg cgtgcgagcg cgtgcccacg ggcacgccgc gccccgtggc
     3121 cagatgcgcc tggcagcagt cctcgacgat cgccagcgcg tgccggtcgg ccacgcgccg
     3181 gatggcgtcc atgtcggcag gctgcccgta gaggtgaacc ggcacgatcg ccttcgtccg
     3241 cgagttgacc gccgccgcgc aggcggacgc atcgagcgtc agcgcgtccg gctcgacgtc
     3301 cacgattcgc ggccgtgcgc cggccgcgac gacggccagg cccgtgaacg cggccgtcat
     3361 cgccgggatg atgacttcgt cgccaggccc gacgccgagc gcgcgtagcg ccagtgtgat
     3421 cgcatcggtc ccgttgccga cgccgacggc ggcgggtgcg ccgcacgccg acgcgaactc
     3481 cgtttcgaac gcctcgacct cggggcccag cacgaaccag ccgctgtcca cgacgcgcgc
     3541 gatcgccgcc ctcacggcgg gcgcatcttc acccggatga aggtctatga agggtaaagc
     3601 cctggacgac gtcacaatgc ggcgaccggc gacccagtcg gcacatagtg cggcaggtgc
     3661 gcgcggtaga actcgatcgt gcgtctgagt ccgtcgcgga gcggagtggt cggacgccac
     3721 ccgagcgtgc gcgcgatgag ggaggagtcg gcgtggaagt cgccgatgtc aatcgccttc
     3781 ttctcggggg gccaggcaat caggcgatag cggccggttc ccgcgaccga gatcagcagc
     3841 tcgaccagct cccgcagcgt gatcgcttcc tgcccgccca cgttgaacac ctgcccgttg
     3901 caggcatcgc tggcgcccgc tcgcagaaac gcgtccgccg catcgtccac gaagacgaaa
     3961 tcacgcaact ggccgccgtc gccgtagatc tggatctcgc gattctcgat cgcctggcgg
     4021 atgaaccagc cgatgaaacc ctggcgattg tgcttgatca gctgccgcgg tccgtacacg
     4081 ttcgtcagcc ggagcgagac ggtgcgcacc ccgaagacgt tgttgtacat catgtggtag
     4141 tactcgccgg cggccttgtt gatgccgttc acgtcggtcg gcctgaccag atgcgcctcg
     4201 gtgaccggca gcgcgtcggc gcggccgtag atctgccggg tgcccgcgaa caccaccttc
     4261 accgtcgggt tgttgtgccg gcatgcctcg agcagcgaca actgcgcgcg gcagttgatc
     4321 tcgagatcgg tgtacgggtc ctgcatgctg tcgatgtggc tcacctggcc ggccaggttg
     4381 aagatgacgt cgtggccgcg cacgaggtag ttcatcgtgc tctgctgccg gatgtcggcg
     4441 atgttgacgc ggaggcggtt ctcgagcccg tcgatgttga acaggttgcc cccgtaatcg
     4501 gagatcaggg agtcgatgac gagcacgcgc gcgccaagcg cgacgagctg acgggccagg
     4561 ttgctgccga tgaacccgag ccccccggtg atcaggacct ggcgcccctg gtagaacgcc
     4621 aggtagtcct ggtcggtcac gggcgggccc caggcgtcgt gggtccgggt ggagacgggc
     4681 gccgcacggc cgggttgctg aacaggacgg gcgtgctctg ccgcccccgg cccagcacca
     4741 cgagctcgat ccacagcttc agcacgtcta tcccggtacg gaacacccgg cggaagttga
     4801 agaactgcga gcgaccgtag ctgcggtggt agtggtggac gggcacctcg accacccgaa
     4861 ggccggcatc ctggatcttg cgcatcagct ccaggcagat cacgccgctg tcgcgatcga
     4921 gcttcacgcg atcgaagacc tcacggcgca tgaggcggaa gtcgcagtcc acgtcgcgca
     4981 cgcgcagggc gaagagcagc ttcaccgtgt agtggtagat ccggccgatg aggatccggt
     5041 gcagcggatc cgaccggctg atcttgtagc ccgtcacgac gtcggcatcc gggcccaggc
     5101 gcgcccacag cgtcgcgatc tccgacgggt cgtactgggc gtcgccgtcg gtgtaggcga
     5161 tcagctcctt cgtggcggac gcgaaacccg ttcgcagcgc cccgccgtag ccgcgattgc
     5221 tcacgtggtg gatgacgcgc acgttgggat aggtccgcgc gagctcgtcg gcgatctgcg
     5281 cggtctggtc ccggctcccg tcgttgatca cgagcacttc gaagtccggg gtcaggtgtc
     5341 gaatggcttc ctgcgcctgg atcacaaggc tggcaatcgt gcccgcgtcg ttgtacgcgg
     5401 gaaagaacat cgtgagaccg ggggcgcgcg tcgcggggcg ttccggcatt gggcgttaaa
     5461 ccgtagcatg ggaaggtttt ccgttacaag gatgggtcta ctcgtagcga agcgggagtt
     5521 gattacgtcg cgagcgggag ctgattacgt cgcgaagcgg aagttgatca cgtcgccgtc
     5581 gcgaaccacg tactccttgc cttcgagccg cacctcgccg tgttcgcggc acgcggccat
     5641 cgagccgcgc gcgacgagcg cctcgtaggc gaccacttcg gcgcggatga acccgcgctg
     5701 aatgtccgtg tggatttcac ccgccgccgc ctgcgcggcc gtgccgcgcg cgatcgacca
     5761 ggcccggcac tcgtcctcgc ccaccgtgaa gaacgacatg tacccgagca ggtcgtagct
     5821 cgagcggatg acgcggtcca ggcccgattc gctcaggccg aggtcggaca gaaacgcctg
     5881 cgcgtcggga ccttccagtt gcgcaatctc cagttcgatc ttcgcgcaca gcgcgacggc
     5941 tcccgttgac gcgcgggcga gaaagcctgt cagccccgtt cccgccgcgg ctttcccgag
     6001 gtccgacccg acggccgccg cttccgattc gtcgagattg atgaccagca gcagcggttt
     6061 tgccgacagg aactgaaacc cgcgcagccg cttgaggtca tctcccgaga tgtccagcgc
     6121 acgaagaggg cggccctcct cgagcgcggc cgtgcagcgc cgaacgaggt cgcgctcctt
     6181 ctcggcctcg gcggagcggc tcttcttcag atccttctcg atgcgctcga gccgccgctc
     6241 ggccactccg aggtcggcga ggatgagttc gtcctccatc gcctgggcgt ctcgcgccgg
     6301 atcgatcgat ccggacgggt gcgccacggc gtcgtcacgg aatgcgcgga ggacgtgaac
     6361 gagcgcatcg gcattcttgt agccggccag atcgacgagc gcctgcacgc ccgtgcgggc
     6421 cggagccacg acatcggtga actcgacggt ggcgggcacc cgtttgcgcg ggttgtacat
     6481 cgccgtgagg acgtcgagcc gggcatcggg caccttcgag atgccgatgt tgacgtcccc
     6541 cttgccgcga ggcgcgtcgc gggcactcgt catgagctgg aacagtgtcg tcttgccact
     6601 cgatggcacg ccaatcaggg ccgctcggag catgagcgag gagcctagca cgcgggtccc
     6661 ctcgtggcgg ggggctccgg ctccccaatc tgttgggtac ggggttggcc agaaaacgaa
     6721 gttctggcga aagtttggcg taactcacat tagatcaggg gtttgcggtt gattgcggtt
     6781 gacttcctga ggctccgcac tttactatcc gtcgtggatt tttctggagt ttagacggtt
     6841 agattggagc gtagtggagc agaaggggat gcgcgggagc gctccggcca ggattgatga
     6901 caaaggtcgg ctcaaggttc ccacgatgtt cagggcggtc attcaggacc agcagggggc
     6961 tgacgtgttc gtcacgagcc tgtcggggga gtccgtccgc atttaccccc tcgccgtgtg
     7021 gatcgagatc gaacggaagc tgatggagat gccaggcaac catccctcgc gcctgaagtt
     7081 tctcgatcgc gtgaactact acggccaggc cagcgagctc gatggccagg ggcgcgtcgt
     7141 catcccgcag cacttgcgcg agagcgcctc gatcgtcgga gacgttcgcg ttttcggccg
     7201 catctcctat ctggaagtgt ggaacgacca gcgcttcctc cagaaactcc agcgtgaggc
     7261 gtggactgac gaggacggac ttcggctgtc cgagcacgga gtgtgagttt tgagtcttga
     7321 gttttgagtt ttggttgagc acgtccccgt catggtccag gaggtgctcg agcatctcga
     7381 gccatcgcgg ggcgggctgt tcatcgactg cactgtaggg ctcggcgggc acgcgcgcgc
     7441 gattctcgac gcgggcgcct cgagggtgat cggactcgat cgggacccgc aggcgctcga
     7501 acgggcgaga gagacgctgg gcgaggtagg cgacagagtg gagctggtac acgccgacta
     7561 ccgtcgattc gaggacgtgc tgaacgccag gggcgtgggc ccggtggccg gcatcctggc
     7621 ggatctcggc gtgtcgtcgc tgcagctcga ctccccgggc cgcgggttca gcttcaggcg
     7681 cgacgagccg ctcgacatgc ggatggacac ctcgagcgga gcaacagcgg ccgacctgat
     7741 tgcgtctgcc gacgagcaaa cgctggcgga cgtgctctac acgctggggg aagagcgata
     7801 cgcgcggcgc gtggcgcgcg cgattctcgg ggaaagcaag gagcggcgga tcgacacgac
     7861 cgggcagctt gccgcgatcg tgaggcgcgc gattccccgc aagggtttct cgcgaatcga
     7921 tcccgcgacg cgcacgttcc aggcgatacg catctgggtc aaccaggaac tggaagggct
     7981 cgacgcgttc ctggtgcggg ccgcggggcg cctcgctcgg ggaggccgtc tcgtcgtggt
     8041 cacgttccac tcgctcgagg accgcgtggt gaagcacacg ctgcgggcgc tggagtcatc
     8101 ggccacgctg gccgtgcgaa ccaggcgccc gatggtgcct tcggaagcgg agatcgggcg
     8161 gaatcctcgc gcgcggagcg cgaagctcag ggcggcggag cggcaggcga cgggtgacgg
     8221 gggcggccat gacgtttgag agcttcgagt actcgatcaa gaaggacatc cggaacaacc
     8281 cgatcatccg tgaggtcgat cgcgagcggc accgggagat gtggcgctcg atgttcatcg
     8341 gtgtgttcct cgtcgcggtt ctcctgtttt ctgcgtggca gcacttcgag ctgcagcagc
     8401 acggacggcg acacgagcag atgctgcagg aacgcgccga cgcggagttc gtcaacctgc
     8461 acctgcgcct cgagatcgag acgctgaagg cgccggcgcg gatcgagggc ttcgcaacgc
     8521 ggcggctccg aatggtggcg ccggcggcgg aggattcgac ggtcatcgag cgcgtgaccg
     8581 cggctcccgc gccgcccacg tcggtcgtcg cgcaacggta gtctcggaac ggagcttcgg
     8641 gtggccgaca agccctccat cgagtggcgc atcacgctga agcggaggct gaccattgcc
     8701 gtctgcctgc tctttgcgtg ggcgggagcg atcgaggcgc ggctcatcta cctccaggtc
     8761 ttccgtcacg ccgactttgc cgcgcgcgcc gagcagcagc agtcccgcag aatcccttcc
     8821 cccgccaagc ggggtgagct ccttgatcgc cacgggaaga tcctggcgcg cagcgttgac
     8881 gccgatagcg tgtacgcgga tccgtccgaa gtcggcgatc ccgatcgggc cgccgccagg
     8941 ctgtgcgagg cccttcgcga ctgcgacgcc gacgatcgcc gcgggatcgc cgaccgcatc
     9001 cgcaagggca ggtacttcgc acacgtccag cgtcaggtca cgcatgagca ggcgcgccgg
     9061 gttgcggagc tcaagctcga gggtgtcggc ttcatcaagg agagccggcg ctactacccg
     9121 aaccgcgatc tcgcctcgca actgctcggc tacgtcggca tcgacaacgt cggcctgagc
     9181 gggatcgaaa aggcctacga ccggatcatc ctgggaaagc cagggacggt cgtggtgcag
     9241 accgacggga accgtcaggc gttcagccgc gaggagcgtc cgccgaccac cggggcgtcg
     9301 atcgagctca cgatcgatca gtacatacag cacgtcgcgg aacgagagct cgaggccgga
     9361 gtcaaatcca gtggggcctc gacgggctcg gccgtggtca tggatccgca tacgggtgag
     9421 cttttcgcgc tggcgagcta tccgggcttc aacctcaaca cctatcgcga ggcgcgcccc
     9481 gacgcgcgcc gcaatcgcgc ggtgcaggac tactacgagc cgggatccac cttcaagatc
     9541 gtgacggcga gcgcggctct cgaggagaag gccatcagcc cggaggaatt gatcgacgcg
     9601 agcgccggaa acatcaggtt cggctcgcgg gtcatcaacg acgatcacaa ctacggcgtg
     9661 ctgtcgttca gcgacgtgat cgtcaagtcg agcaacgtgg gcgccatcaa agtggcgctc
     9721 aagctcggcc cccgcaagat gggggactac gtgaagcgct tcggcttcgg caggccgtcc
     9781 tccccggact tccgcggaga aagccccggc atggtctggg atccctcctc gctgaacgac
     9841 agcgccctcg catcggtcgc gatgggatac caggttggcg tgacggctct gcagatggca
     9901 gccgccgtca gcgtcgtggc caacggcggg gagctcgtcc agccgcgcgc ggtgcgcgcg
     9961 gtcattcacg acggcaagcg cctgcccgtg cctcacaaga ccctcgagcg tgtcatcagc
    10021 cgcggcacgg ccgtcaccat ggccgagatc atggagaagg tcgtcgagga aggcaccggt
    10081 acccgcgcca ggattcccgg ctacaccgtt gccggcaaga cgggcaccgc caagaagctc
    10141 gtccgcggca gctatcgcgg ccactccgac tacaacgtgt cgtttgtcgg gttcgtcccg
    10201 tcgcgggcgc cggtcttcgc catcgtcgtc gtcgtggacg ccccgcacag ggtttcgccg
    10261 tacggcggcg tggtggctgc cccgattttc cagaagatcg ccgacgccgc gctcaggcac
    10321 tacggtgtat ccccgtcgat caacgcgcct gaaccggtgc tcgccggccg ctgggaagag
    10381 cgcctcgagc tcacggccgg tccggcggct ccccgcgcca tcgttccgct cgggggctcc
    10441 tccggcgagg tgtttcccga cctcaccggg ctgagtgctc gcgaggcgac ggcgatcctg
    10501 acacgcctgg gttacacggt tcggttgcgc ggtgacgccg ggttggtcgt agagcagaca
    10561 cccgagccag gcacgccgct cgagtcgagt acgacggcgg tgctcacggt cgaacgccgc
    10621 gccgcggatc tcgaatcgtc cgccggcgtg ccatgacggt tggagatgtt gtccgtgctg
    10681 tcgcgcgtga aggcgaaagg cagctcgcag gttcctcgtc gggtctcgat cggccctgca
    10741 cgggcgtcac gcacgattcg cggcgcgtcg agcccgggac cgtgttcgtg gccttgcggg
    10801 ggctcaaggc ggacggcgcc gcgtttgtgc ctcaggcgat cgccgcgggt gccgctgcaa
    10861 tcgtggccga gcgcgggccc gagggctccg ttggcgttcc gtgggttgtc gtcccggacg
    10921 cgcgcctggc gctggctctc gcgtcggcag agtttttcgg ccacccgagc cggcagatgc
    10981 aggtcgtggg catcacgggc accaacggga agacgacgac cgcgtacctg gtgagagcga
    11041 ttctggagac cgctggggtg aagagcggcc tgatggggac ggtgacgtat caactgggcg
    11101 accggtcgtt cgacgccacg cggacgacgc ctgaagcgcc cgacgtgcag ggcttcatgc
    11161 gggagatggt cgcgtcgggg tgtcgcgcct gcgccatgga ggtctcgtcc catgcgctgg
    11221 ctctcaggcg cgtggacgga atccgcttcg ctgccgccgt gttcaccaac ctgacgcggg
    11281 atcacctcga cttccattcg gacatggacg actacttcgc cgccaagcgg cggctcttcg
    11341 acatgctgcc ggagggcgcc ccggccgtca tcaacgccga cgacccccgc ggcgcttctc
    11401 tcgtggattc cgtccggcgg cccgtgacgt acggcatgac gaggacagcc gacgtgtcgc
    11461 ccggtcccat tgcctactcg ctggacggtc tgcagttcga catccgcgct ccgaaaggcg
    11521 ccgttcgcgt gcggtcgagg ctggtcggca agccgaacgc ctacaacatc ctggcggccg
    11581 tagcgaccgc gaccgagctc ggggtcccca ccgaagccgt cgaggccggt ctcgagcgtc
    11641 tcgagggtgt tcccggccgc ttccagatgg cctcgggtgc atccgatgac gtgcgcgccg
    11701 tcgtggacta cgcgcacacc gacgacgccc tgcggaacct gctcgagacc gcgcgcccga
    11761 tggcgacgcg ccggctgatc accgtgttcg ggtgcggcgg cgatcgcgat cggaccaaac
    11821 gtccgctgat gggaatggtg gcggctcgcc tcagcgacgt cgtcgtgatc acgtccgaca
    11881 acccgcgcag cgaggacccg aaccagatca tcgaggagat caggcgcggt gccgacgccg
    11941 agaccaggca gagccgggcc gaggtgttga tacgtccgga caggcgcgac gccattcgag
    12001 acgccgtcga gctggccggt ccaggcgacc tggtgctcgt cgtggggaag gggcacgaga
    12061 agtaccagga gatcgggggt cgcacgttcg cgttcgatga cgtggcagtg gcgaaggagg
    12121 cgctggaggc gcggcggctg aagtcgcggg ccgggtgacg ggcgtttcca tcacgctcga
    12181 ctgggtcgcc agcgcggttg gtgcgacgtc tcgcgccggt gccggcggcg cggtcgtcgg
    12241 cgatgtggtc accgacagcc ggacgttgaa accgggtgac ctcttcgtcg cgcttcgagg
    12301 tccgcggttc gacggccacg cgttcgcagc cgaggtgctc gagcgcggcg cggcgggggt
    12361 gatcgtcgag cgtggacggg cggctggcga cgcgcacgcg atcgaggtcg aggacacgct
    12421 cgcggcgctg cagggcctgg cgcacgcggt caggaaggcc gtgggcacgc gggtaatcgc
    12481 gatcaccggc agcgcgggga agacgacgac gaaggaggcc attgcggcgg tgctggccac
    12541 gcgatatcgg gtggtcaaga acaaggggaa cttgaacaac cacattggac tgccgctgtc
    12601 cttgatgcag ttgcggacgg cgccggacgt ggcggtgatg gaactgggca tgaaccacgc
    12661 gggtgagatc agcaggctcg tggcgatcgc ggaaccggag gttcgcgtgt ggaccaatgt
    12721 cggtgacgcg cacctcggat tcttcgagtc tgccgacgcg atcgcggatg ccaaggcgga
    12781 gattctcgaa ggcgccaccg ccgatgccgt gctcgtctcc aacgcggacg acgcgcgcgt
    12841 cgcggcgagg gcgggccgct ttcccggcag gaccatcacc ttcggggagt cgcccggcgc
    12901 cacggtgcgg gcgctctcga cgaggctgca tggcgttcag ggcgtgagcg ccgatgtcgc
    12961 gacgccagcc ggcacgcgct cgctggcgac gccgctgctt ggccgcggaa acatgtggaa
    13021 cgtcctggcg gcaaccgccg tggggctcga gttcggcctg gacctggacg ccattgcggc
    13081 cgaggttgcc cgcttgcagc cggccgatcg ccgcggcgtt gtgcggcatc tccgcgacgg
    13141 gatcacgctt gttgacgatt catacaactc gagtccatcc gcccttgccc gcgcgctcga
    13201 cgtcgtcgcg tacgaagcgc acgcgaggcg ggccgtgggc gtgctcgggg agatgctcga
    13261 gctgggggaa catgcgcgcg tcctgcacga ggccagcggc cgagccgcgg ccgctgccgg
    13321 gcttcggcgg ttgttcgtcg tgggtggcgc tgccgcgcgc gcgatggctg acgcagcggt
    13381 cgcagaaggc atgccggcgt cggcggtgtc ctacttcgag aggagcacgg aggccgcacc
    13441 tgccgtggtg gccgccgtcg aagcgggcga tctggtgctc gtcaaggggt cgcgcggcac
    13501 gcgcacggac gtgatcgcgg accagctcgt gacagaactt ggctgatgct ctattacctg
    13561 ctgccgccgc ttgcgacgct cgcgccgttt ctcggcgtgt tgaacgtgac gcgctacatc
    13621 acgttccgca cggcggcggc cagcctgacg gcgctcgcca tcagcctgct gttcggtccc
    13681 tggttgatca g
//
LOCUS       DBNGSH010000261         2737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_869512, whole genome shotgun sequence.
ACCESSION   DBNGSH010000261 DBNGSH010000000
VERSION     DBNGSH010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_869512"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="AC16CY_06010"
     CDS             <1..365
                     /locus_tag="AC16CY_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353372.1"
                     /translation="DNVRNNAVRALGVLLQARPELARDVPAASVLPLLSSPVWSDQNK
                     GAMLVEALTKNRNPELLRQLRASALDNLLEMARWRSAGHAESARMLLGRIAGIEEDRL
                     LKLVASGDVEAIIRALSR"
     gene            complement(413..1408)
                     /locus_tag="AC16CY_06015"
     CDS             complement(413..1408)
                     /locus_tag="AC16CY_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024211.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3353373.1"
                     /translation="MMSRSRRFLFILSCALTFGAGILRQDAALVLAQTTEPPAQGGWT
                     FEEDEDAAPTWGDDIRAQAADLAGVAAFTALAFVSFFRKSVRLKYVTLGVAIVYLGFY
                     KSLLISIVNVFALIGGGLPIFRYNLAWYLLAAITIVSTVLWGRVYCGRICAFGALTQL
                     MDQVVPARWRVNVPRALERRAAWIKYGLLAGVLAYFLATKDSSVYPYVEPFWLFGIYG
                     RSAALWAGLALLLIATVFVRNLYCRFLCPLGAALGILSNLTVFRIKRWSECKTCKICE
                     KACEWGAIQGPKIVLTECVRCDDCERLYADTTKCPHHLILLRKRPQGPGLRRPQA"
     gene            complement(1405..1905)
                     /locus_tag="AC16CY_06020"
     CDS             complement(1405..1905)
                     /locus_tag="AC16CY_06020"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR3353374.1"
                     /translation="MKRLAVFLTLIIGVSVIVPAQGQPDARLKRLFPSATSFSPKEGD
                     PPHFKAFNGKTLLGYAFWTTEVSPLERGYAGPIAIVVGLDLKGTLTGIVVADHHEPYG
                     DFSIDRPGFASQFAGKDVRDPFKLGEDIDAVSRATITMSSAVRAVRGSARRVARQFLA
                     PPSPSK"
     gene            1930..2409
                     /locus_tag="AC16CY_06025"
     CDS             1930..2409
                     /locus_tag="AC16CY_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353375.1"
                     /translation="MQISFLARRTAAGALLVLTTAIAFAQNADPPNPLEKVKALKCRF
                     PAATSAVWKAGEPQAQVKMQDMRFDITAIDVQDGTAEFMGTAGRAYVTAVLSGWSVYF
                     VESAVGQLNITTVFAQEASPKKLKAVHSRHGYLQMQVGRFIAEPSVSQNYGECEIAE"
     gene            2417..>2737
                     /locus_tag="AC16CY_06030"
     CDS             2417..>2737
                     /locus_tag="AC16CY_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353376.1"
                     /translation="MTLILHNLPPLHWALAGVAIAGVTLALLFLSSRRLGISTGFEDI
                     CSLVLTAPYFRRAAVLSGRGWRLPLLAGLVIGGFLSAVLGGGWEPTWALGVFDRAIGL
                     GPAGK"
BASE COUNT          490 a          912 c          904 g          431 t
ORIGIN      
        1 acgacaacgt ccgcaacaac gccgtgcgcg cgctgggtgt gctgctgcag gccaggcccg
       61 agctcgctcg cgatgttccg gccgcgtcgg ttctcccgct tctgtcgtct cccgtctgga
      121 gcgaccaaaa caagggcgcc atgctcgtcg aggcgctgac caagaaccgc aatccggagc
      181 tgctgcggca actgcgcgca agcgcgctcg acaacctgct ggagatggcg cgctggcgca
      241 gcgcagggca cgccgagagc gcgcggatgc tgctcggccg cattgccgga atcgaggagg
      301 atcgtctgct gaagctcgtc gcgagcggcg acgtggaggc gatcatccgg gcgctttcgc
      361 gctgaccaag gccgcagggc tcaggcgtca ggccccaggg cagggctagg cttcaggctt
      421 gtggccttct gaggcctggg ccctgaggcc tttttcgtaa gaggatcaga tggtgcggac
      481 acttcgtcgt gtccgcgtag agccgttcgc agtcgtcgca gcggacgcac tcggtcaaga
      541 cgatcttcgg cccctggatg gcgccccact cgcacgcctt ctcgcagatc ttgcaggtct
      601 tgcactcgga ccaccgcttg atccggaaca ccgtcaggtt cgagagaatc cccagcgcgg
      661 ccccgagcgg gcacagaaac cggcagtaga gatttcgcac gaacaccgtg gcgatgagca
      721 gcagggcgag tcccgcccac agcgccgccg agcgcccgta gatgccgaac agccagaacg
      781 gctcgacata cgggtagacc gacgagtcct tcgtggccag gaaataggca agcacgccgg
      841 cgagcagccc gtacttgatc caggccgcgc gccgttcgag cgcgcgggga acgttgaccc
      901 gccagcgcgc cgggaccacc tggtccatga gctgcgtcaa cgcgccgaag gcacagatcc
      961 ggccgcagta cacgcgcccc cacagcacgg tcgagacaat cgtgatcgcc gcgaggaggt
     1021 accacgccag gttgtaccgg aagatcggaa gccccccgcc gatcagcgcg aagacgttga
     1081 cgatcgagat gagcagactc ttgtagaaac cgagatagac gatggcgacg cccagcgtga
     1141 cgtacttgag ccgtacgctc ttgcgaaaga agctgacgaa cgccagcgcc gtgaaggccg
     1201 ccacgcccgc caggtccgcc gcctgcgcgc ggatgtcgtc gccccaggtt ggcgccgcat
     1261 cctcgtcctc ctcgaacgtc caccctccct gggccggagg ctcggtcgtc tgcgcgagga
     1321 cgagagccgc gtcctgccgc aggatcccgg cgccgaacgt caacgcacac gacaggatga
     1381 agaggaatcg ccgcgaacga gacatcattt actgggtgat ggcggcgcca ggaactgccg
     1441 cgccacgcgg cgcgcgctgc cgcgcacggc gcgcacggcg ctcgacatgg tgatcgtcgc
     1501 ccgcgagacg gcgtcgatgt cctcgcccag cttgaacgga tcgcgcacgt cctttcccgc
     1561 gaactgcgag gcaaagccgg gccgatcgat ggagaaatct ccgtagggct cgtggtgatc
     1621 ggcgacgacg atgccggtca acgtcccctt caggtcgaga ccgaccacga ttgcgatcgg
     1681 gccggcgtaa ccgcgctcga gcggcgacac ctcggtcgtc cagaacgcat aacccaggag
     1741 cgtcttgccg ttgaacgcct tgaagtgcgg agggtcgcct tctttcggcg aaaaagaggt
     1801 cgccgacgga aagagccgtt tcaaccgcgc atcgggctgt ccctgggccg ggacgatgac
     1861 cgacaccccg atgatcagcg tcagaaaaac ggcgagccgc ttcatggacc catggattat
     1921 aagtggacta tgcagatctc cttcctcgct cgccggactg ccgccggcgc actgctcgtc
     1981 ctgacgacgg cgattgcctt cgcgcagaac gcggacccgc cgaacccgct cgagaaggtg
     2041 aaggcgctca agtgccggtt tcccgccgcc acgtcggccg tctggaaggc cggcgagccg
     2101 caagcgcagg tcaagatgca ggacatgcga ttcgacatca ccgccatcga cgtccaggac
     2161 gggacggccg agttcatggg aaccgccggg cgcgcctacg tcacggcggt cctgtctggt
     2221 tggagcgtgt acttcgtcga gagcgcggtg gggcagctga acatcacaac ggtcttcgcg
     2281 caggaagcgt ccccgaagaa gctgaaagcc gtgcactcgc gtcacgggta tctgcagatg
     2341 caggttggcc gcttcattgc cgagccgtcc gtgtcgcaga actacggcga gtgcgagata
     2401 gcggagtaga ggtgcgatga ctcttatcct tcacaatctc ccgccgcttc actgggcact
     2461 ggccggcgtc gcgatcgccg gcgtcaccct cgcgctgctc ttcttgagca gccgtcgcct
     2521 gggaatctcg actgggttcg aggacatctg cagcctcgtg ctgacagcgc catacttccg
     2581 gcgcgccgcc gtcctttcgg gccgcggatg gcggcttccg ctgcttgcgg gcctcgtgat
     2641 cggcggcttc ctgtctgccg tcctcggagg cggctgggag ccaacgtggg cgctcggcgt
     2701 cttcgatcgc gcgatcggcc tcggcccggc gggcaag
//
LOCUS       DBNGSH010000262         5048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1208675, whole genome shotgun sequence.
ACCESSION   DBNGSH010000262 DBNGSH010000000
VERSION     DBNGSH010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1208675"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC16CY_06035"
     CDS             complement(<1..245)
                     /locus_tag="AC16CY_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353377.1"
                     /translation="MPRTAAVVRAAHVAAVTVLSAGCLVGPNYKRPELAPPQQFRFVD
                     GPQAESLANAPWFTVFEDPALQALIKDAIANNLDLRQA"
     gene            complement(247..3438)
                     /locus_tag="AC16CY_06040"
     CDS             complement(247..3438)
                     /locus_tag="AC16CY_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3353378.1"
                     /translation="MARFFVNRPIVAMVISIITVLLGVVAMSGLPISQYPEIVPPMIQ
                     VTTTFIGASSTDVEASVATPLEQKINGVENMIYMKSTNANDGTLTLKVSFEVGSNLDM
                     SNVLTQNRVSEGMPQMPQSVKNYGVSVKKALAFPLLVISIKSPKGTYDSAFLSNYTTI
                     NINDAIARIQGVGQINLFGGSDYAMRVWLRPDVIGRLGVTIPDIANAISQQNQLTPAG
                     QIGGPPAAEGTEYTYTVRTQGRLLNEEEFSNIIVRTNPDGSEVRLKDVARIELGTMLY
                     NSIGRHDSTPSAVIAVFQIPGTNALDVANRIKATMEELKQRFPRDMEYLVSLDTTLPV
                     VEGIREIEHTLFEAVALVIIVVFVFLQNWRATLIPLLTVPVSLVGAFIFFPLLGFSIN
                     VLSLLGLVLAIGIVVDDAIVVVEAVMHHIEHGMAPKEATIKAMEEVSGPVVAIGLILA
                     AVFVPVGFMGGITGLLYQQFAITIAISVLLSVINALTLSPALAALLLKPATGKKTLLT
                     PFYNGFNRVFGWSTDRYVSFAGILARKMVRSLAFIGVLCYVTFMLVQRIPGGFVPEED
                     QGYLLVNALLPDAASLERTDAVMRKVEAILEKNEAIEGFNTITGFSLLTGAYSSNMGF
                     FFVQLKPWEERTTEESHANGVIAALNRGFAQSIPEAGVVAFGPPAIPGLGTGAGFTMQ
                     LQDRSGGSPEYLARQAALFMEAARKRPEIQRINTLYRAQVPQIYADIDRSKVLKAGVP
                     LSDVNTTLGALLGSSYINDFNRFGRVYKVYVQAEPEFRQDPKQLGLFYVKNQKGGMVP
                     LDTLLSIGPGNGPEFTNRFNLFRAAELTGVPAEGFSSAQALDALEATAREVLPPDITF
                     DWADMSFQERRAPGVATVFALAIFLVFLILAAQYESWGLPFSVLLGTPFAAFGAYLGL
                     YLARLFMGPSYVNNVFAQIGLIMLIGLAAKNAILIVEFAKMELEAGKPLMEATMSAAK
                     LRFRPILMTAFAFILGVVPLLTATGAGAEGRKVMGMTVFAGMLVATILGVCLIPMLFV
                     AVEKLTGGSKHPAPATPAVPPQSLATEHGHGGH"
     gene            complement(3441..4604)
                     /locus_tag="AC16CY_06045"
     CDS             complement(3441..4604)
                     /locus_tag="AC16CY_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3353379.1"
                     /translation="MGAHRTTLLAATMAAAALAGACEEPAAVAPPPPEVYVTDVVQRD
                     VPVYLDLVGQTAGFQDVDIRARVEGFLETVNFQEGSFIKKGDLLYTIDRKPLEAILAQ
                     GKADQATAEARLSKANNDVARYTPLVAKQAVSKQELDNALAEQDASKSQVEAAKAAVE
                     KASLDLGYTRVTSPISGLVGTTQVKSGNLVGRGESTLLTTISQIDPILFTVGVTEADY
                     LRVAKQVTAAGKSSGGSREGIELRLADGAVYAHTGKISTIERAVDPSTGTLGMELVFP
                     NPDQILRPGQYGRARILLDTKRGALLVPQRAVQELQSIYTVAIVEGGKVAFRTVKVGQ
                     KVDSLWVIEEGVKAGEQIVVEGLQRIQDGMTVSAKPAPATPAGSAAPAAVEAK"
BASE COUNT          886 a         1633 c         1642 g          887 t
ORIGIN      
        1 gcctgccgga gatcgaggtt gttggcgatg gcgtccttga tgagcgcctg cagcgcggga
       61 tcctcgaaga ccgtgaacca gggcgcgttg gcgagcgact cggcctgtgg gccgtcgacg
      121 aagcggaact gctgcggagg cgcgagctcg ggccgcttgt agttcggacc gacgaggcac
      181 cccgccgaca ggacggtgac ggccgcgacg tgcgcggccc ggacgactgc agccgttcgc
      241 ggcatctcag tggcccccgt gcccgtgctc ggtcgccagc gactgaggag gcacggccgg
      301 cgtggccggc gcggggtgtt tcgacccgcc ggtcaacttc tccacggcca cgaacaacat
      361 cggaatcagg cacacgccga ggatggtcgc gacgagcatg cccgcgaaga cggtcattcc
      421 catcaccttg cgcccctcgg cgcccgcacc cgtcgcggtc agcagcggca cgacgccgag
      481 gatgaacgca aaagccgtca tcaggatcgg gcggaagcga agcttggccg ccgacatcgt
      541 cgcctccatg agcggctttc ccgcctcgag ctccatcttc gcgaactcga cgatcaggat
      601 cgcgtttttc gcggccagac caatgagcat gatcagtccg atctgcgcga agacgttgtt
      661 cacgtaactc ggtcccatga acagccgcgc cagatagagg ccgagatacg cgccgaacgc
      721 ggcaaacggt gtccccagca agacgctgaa cggcagaccc cagctctcgt actgagccgc
      781 caggatgagg aacacgagga agatcgccag ggcgaacacc gtggccacac caggggcgcg
      841 tcgttcctga aacgacatgt ccgcccagtc gaacgtgata tccggaggca gtacctctcg
      901 agccgtggct tccagcgcgt cgagcgcctg cgccgacgag aaaccctcgg ccggcacgcc
      961 ggtgagctcc gccgcgcgaa acaggttgaa gcggttggtg aactcgggtc cgttacccgg
     1021 tccgatcgac aatagcgtgt cgagcggaac catgccgccc ttctggttct tcacgtagaa
     1081 cagcccgagc tgtttcggat cctgccggaa ctccggttcg gcctggacgt acaccttgta
     1141 gactcgcccg aaccggttga agtcgttgat gtaggagctg ccgagcagcg cgccaagcgt
     1201 cgtgttcaca tcgctgagag gcacacccgc cttgagcacc ttgctgcgat cgatgtcggc
     1261 gtagatctgc ggcacctggg ctcgatagag cgtgttgatg cgctggatct cgggccgctt
     1321 ccgcgcggcc tccatgaaga gcgcggcctg ccgcgccaag tactccggcg aaccgccgct
     1381 gcggtcctgc aactgcatgg tgaacccggc ccccgtgccg agtcccggaa tggccggcgg
     1441 cccgaaggcc accactcccg cttccggaat cgactgcgcg aacccgcggt tcagcgccgc
     1501 gatcaccccg ttggcgtgcg actcctcggt cgtgcgctcc tcccacggct tgagctgcac
     1561 gaagaagaag cccatgttcg acgagtaggc gccggtgagc agcgagaagc cggtgatcgt
     1621 gttgaagccc tcgatcgcct cgttcttttc gaggatcgcc tcgaccttcc tcatgacggc
     1681 atcggtacgc tcgagggagg ccgcatccgg caacaacgcg ttcaccagca ggtatccctg
     1741 atcctcttcc ggcacgaacc cgcccgggat ccgctggacc agcatgaagg tgacgtagca
     1801 gagcacgccg atgaaggcca ggctccggac catctttcga gccaggatgc ccgcgaagct
     1861 gacgtagcga tcggtcgacc agccgaacac gcggttgaag ccgttgtaga agggcgtcag
     1921 cagcgtcttc ttgcccgtgg ccggcttgag cagcagcgcc gcgagtgccg ggctcagggt
     1981 gagcgcgttg atgaccgaca gcagcaccga gatcgcgatc gtgatcgcaa actgctgata
     2041 caacagtccc gtgatgccgc ccatgaagcc cacgggcacg aacaccgccg ccaggatgag
     2101 accgatggcc acgacagggc cagagacctc ctccatcgcc ttgatcgtcg cttccttcgg
     2161 cgccatcccg tgctcgatgt ggtgcatcac cgcctcgacc acgacgatcg catcgtcgac
     2221 gacgatgccg atggcgagga cgaggccgag cagagagagc acgttgatcg agaaaccgag
     2281 cagcgggaag aagatgaagg cgccgaccag cgagacgggc accgtgagca gggggatcag
     2341 cgtcgctcgc cagttctgca aaaagacgaa gacgacgatg atgacgaggg caacggcctc
     2401 gaacagcgtg tgttcgattt ccctgatgcc ctcgacgacc ggtagcgtcg tgtcgagcga
     2461 gaccaggtat tccatgtctc gggggaagcg ctgtttcagc tcctccatcg tggccttgat
     2521 ccggttggcc acgtcgagtg cgttggtacc gggaatctgg aacacggcaa tgacggccga
     2581 cggagtgctg tcgtggcggc cgatggagtt gtagagcatc gtcccgagct cgatgcgtgc
     2641 gacgtccttg aggcggacct cggagccgtc cgggttggtc cgcacgatga tgttcgagaa
     2701 ctcctcttcg ttcagcaggc gtccctgcgt gcggactgta taggtgtact cggtgccctc
     2761 ggctgcgggc ggcccgccga tctgaccggc cggcgtcagc tggttctgct gggagatggc
     2821 gttcgcgata tcggggatcg tgacaccgag ccgaccgatg acgtccggac gcagccacac
     2881 gcgcatcgcg tagtcgctcc cgccgaacag gttgatctga ccgacgccct ggatgcgcgc
     2941 gatggcgtca ttgatgttga tcgtcgtgta gttcgagagg aacgcgctgt cgtaggtgcc
     3001 ctttggcgac ttgatcgaga tgacgagcag cgggaacgcc agcgccttct tcaccgacac
     3061 gccgtagttc ttgaccgact ggggcatctg gggcatgccc tctgacaccc ggttctgcgt
     3121 gagcacgttg ctcatgtcga ggttcgaccc gacctcgaag gagaccttca gcgtcaacgt
     3181 gccgtcgttg gcgttggtcg acttcatgta gatcatgttc tccacgccgt tgatcttctg
     3241 ctcgagcggc gtggcgaccg acgcctcgac gtcggtcgag ctggcgccga tgaaggtcgt
     3301 cgtgacctgg atcatcggcg gtacgatctc ggggtactgc gagatgggca ggccgctcat
     3361 ggccacgacg ccgagcagta cggtgatgat cgagatcacc atcgcgacga tgggacggtt
     3421 gacgaagaag cgggccatga ctacttcgcc tcgaccgctg ccggggcagc ggagccagcg
     3481 ggtgttgccg gagccggctt ggcggacacg gtcatgccgt cctgaatgcg ctgcagtcct
     3541 tcgacgacaa tctgctcacc cgccttgaca ccttcttcga tgacccagag cgagtccacc
     3601 ttctggccca ccttgacggt gcggaatgcg acttttcctc cctcaacgat ggccacggtg
     3661 tagatgctct ggagttcctg gacggcgcgc tgtggcacga ggagcgcgcc ccgcttggtg
     3721 tcgagcagaa tgcgcgcgcg gccgtactgc ccgggacgca ggatctggtc cggattcgga
     3781 aagaccagtt ccattcccag cgtcccggtc gacggatcga ccgcgcgctc gatggtgctg
     3841 atcttgccgg tgtgcgcata gacagcgccg tcggccagcc tcagctcgat gccctcccgg
     3901 gaaccgccgg aggacttgcc ggcggcggtg acctgtttcg cgacccgcag gtagtcggct
     3961 tcggtgacgc ccaccgtgaa caggatcgga tcgatctgcg agatcgtcgt caggagcgtg
     4021 ctctccccgc ggcccacgag gttgccggat ttcacctggg tggtaccgac gagaccgctg
     4081 atcggtgacg tgacccgcgt gtagccgaga tcgagcgacg ccttctccac ggccgccttt
     4141 gccgcttcga cctgcgactt cgacgcgtcc tgctcggcaa gcgcgttgtc gagctcctgt
     4201 ttactgaccg cctgcttggc gacgagaggc gtgtaacgtg cgacgtcgtt gttggccttg
     4261 gacagtctgg cctcggccgt cgcctgatcc gccttgccct gagccaggat cgcctcgagc
     4321 ggcttgcgat cgatcgtgta gaggagatcg cccttcttga tgaacgagcc ctcctggaag
     4381 ttcaccgtct cgagaaagcc ttccacccgc gcgcggatgt cgacgtcctg aaagcccgcg
     4441 gtctggccga ccaggtccag gtacacgggg acatcccgct ggacgacgtc ggtcacgtac
     4501 acctctggag gcggtggtgc gaccgccgcc ggttcttcac acgccccggc cagggcggcc
     4561 gccgccatcg tggcggcgag caacgttgtt cggtgagctc ccatgtctcg catccctgct
     4621 ttcctgtgaa ccctttccac ggctcgaagg cgacaactgc gcgcactggg ccgtcggaag
     4681 agcaacacac agtgtgtgcc accgtcgcga gcccacgcaa ggtgttgaat gcggggcaaa
     4741 acgcccgttc gggctcgccc gcccgcactg ctcgccggag tgtgctccga actctcggag
     4801 ttcggaccgc gggcctgatc gaagagtcgg cgacccggcc aacgactcct cacgacgcgg
     4861 tcccatggca cgggacctgc tggacgcact cccggcactc ccgtagaatc ggcggcctgc
     4921 cgggcgtgca cttggtgcgt ccggggacgt cgacacatgc cacgcatgag gtagaggttg
     4981 gcagcttcag aacgcatcct ggacccgatc gagcgcacga ccgaggtcat gttcggcatc
     5041 atcatggt
//
LOCUS       DBNGSH010000263         4997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_530104, whole genome shotgun sequence.
ACCESSION   DBNGSH010000263 DBNGSH010000000
VERSION     DBNGSH010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_530104"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="AC16CY_06050"
     CDS             complement(<1..485)
                     /locus_tag="AC16CY_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3353380.1"
                     /translation="MSPHRHFVRSFLIVGAAATLFTTAAFSWATLLGQARPAQVDPCA
                     GSRDLRLTNGKIVTMDARGTTVSEVTIQDGRITAVGPRGNQRVSPCTREINLRGRTAV
                     PGLIDNHNHIVLLGIRPGHHTPLEGAASIAEIQGILKARAKSVPAGAFITSMGGWNQA
                     H"
     gene            complement(580..1425)
                     /locus_tag="AC16CY_06055"
     CDS             complement(580..1425)
                     /locus_tag="AC16CY_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HIR3353381.1"
                     /translation="MAGKLVLVAGATGQQGGAVARELLKAGYQVRAMTRKPEGDAAKA
                     LAAAGAEIVKGDLDDAASVSAALKGAWGAFAVQNTWEAGVEGEERQGKQFAELAKKAG
                     VQHLVYTSVGSSHRKTGVPHFENKARIEQTIKSLGFPSYVILRPVFFMQNMKTFFAPD
                     ANGNFYVAMSPGRKLQMIAVADIGKYGRLAFDKAAELNGQAIDIAGDELSMTDAGKII
                     SQASGKPANHVKANIEDVRAYSADMAMMFEWFESTGYDVNIAANEKKYGIKPTSFSEW
                     AKSAY"
     gene            complement(1498..2607)
                     /locus_tag="AC16CY_06060"
     CDS             complement(1498..2607)
                     /locus_tag="AC16CY_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR3353382.1"
                     /translation="MATKIVDKFLPTTMIGSYPRPHWFKHQLLGRDIRVAFKEVQHEE
                     AYDDAVATVIRDQESAGLDIVTDGNMWYDDYVGVIGSFCWYMYERIGGFEPSREPHPS
                     VVNAPPSSGKVILDDWGGVINSGPVTHGPIRLADLYRIASRHATRPVKVSVGAGPANL
                     AWHVYFKHYKNAKDLSFALAPIFNKEMKDLVAAGAKYLQFEDLGAWLPLFTGDKNDYK
                     WIRESVEMCCDGVDAKIGWHFCFGNAWGNDILSANYPEGYQTVLPYFFGTAGIDEFVL
                     DYANRDMAGVDFLKNLPANQGIQLGVLDIRTNAIEQPAKIADRIRRALKHIPADKVTL
                     SSDCGMKPLARMVAKMKLGALAEGAAIVRKEVAGV"
     gene            3057..3707
                     /locus_tag="AC16CY_06065"
     CDS             3057..3707
                     /locus_tag="AC16CY_06065"
                     /inference="COORDINATES: protein motif:HMM:NF026037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3353383.1"
                     /translation="MTLPIGDGTVTGLVKFEGKAPEARVIRMSSDPLCMPEGPTTSEV
                     VLVGPGNGLQNVFVYVKDGLGDRRFAAPKQPVVLDQRGCKYMPHVFGVQVGQPVNIIN
                     SDATLHNVHAVPKANAEFNFGQPSKGMESAKTFNKPEIMVPFRCDVHGWMAAYAGVVE
                     HPFFAVTSSDGKYEIKGLPAGTYTIEAWHERFGVQTQTVKVADKGSATAAFAFKAS"
     gene            complement(3746..4168)
                     /locus_tag="AC16CY_06070"
     CDS             complement(3746..4168)
                     /locus_tag="AC16CY_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3353384.1"
                     /translation="MAFWDASAIVPLCCSQQATTEGRRLRHELKRMVVWWGTPLEARS
                     AFARLVREGELTSVERVKAIHLLRRLRTAWDEILPTEKLRTIAEELPDAHGVRAADAA
                     QLAAALVWCRERPKGRPLVCFDDRLRAAAAALGFSVRP"
     gene            complement(4171..4455)
                     /locus_tag="AC16CY_06075"
     CDS             complement(4171..4455)
                     /locus_tag="AC16CY_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3353385.1"
                     /translation="MRSTNIADLRNKLTRYLQEVRAGEEIVVRDRQRPIAKIVPFSVD
                     DEDADDAALVAGGLMRKGSGTLPTSFWKVRRSTITLQRAAASVRADRDER"
     gene            complement(4519..>4997)
                     /locus_tag="AC16CY_06080"
     CDS             complement(4519..>4997)
                     /locus_tag="AC16CY_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3353386.1"
                     /translation="NVDALPAEHESTIVRVVAEQFAWNVHYPGADGVFGRTDIRLVDP
                     ENPLGLDRTEPPGRDDLVVNGRLNVPVGKPVIVFLSSKDVIHSFGLPQMRVKQDAVPG
                     IVQPVWFTPTMTGEWNIACSQLCGLGHFRMRGIYAIQTQEDYEAWLKEEASYLAAP"
BASE COUNT          825 a         1622 c         1620 g          930 t
ORIGIN      
        1 aagtgcgcct ggttccaccc gcccatcgac gtgatgaacg cgcccgcggg gacgctcttg
       61 gccctggcct tcagaattcc ctggatctcc gcgatcgacg cggcgccttc gagcggcgtg
      121 tggtggccgg gcctgatgcc gagcaggacg atgtggttat ggttgtcgat gaggccgggc
      181 acggccgtgc gcccgcgaag gttgatctcc cgcgtgcacg ggctcacccg ctgattgccg
      241 cgtggaccca ccgcagtgat tcgtccgtcc tggatcgtca cctccgacac cgtcgtcccg
      301 cgagcgtcca tcgtcacgat ctttccgttc gtcaggcgca ggtcgcgtga acccgcgcac
      361 gggtctacct gcgccggccg cgcctggccg aggagcgtag cccacgaaaa cgccgccgtc
      421 gtgaacagcg tggcagccgc gcccacgatc aagaagcttc gaacgaaatg gcgatggggg
      481 gacatatgcg tcctgtcgag atgaaaacgg ccgagcctcc accaggaggc ccggccctgc
      541 tccgatccgc tacaaccggg aaccagccgc gcagagcgct cagtaagcgc tcttcgccca
      601 ctcgctgaag gaggtcggct tgattccgta tttcttctcg ttcgctgcga tgttgacgtc
      661 gtagcccgtg ctttcgaacc actcgaacat catcgccatg tcggcgctgt aggcgcggac
      721 atcttcgatg ttcgccttga cgtgattggc gggcttgccc gaggcctggc tgatgatttt
      781 cccggcgtcg gtcatgctga gctcgtcgcc ggcgatgtcg atcgcctgcc cgttgagctc
      841 ggcagccttg tcgaaggcga ggcgcccgta cttgccgatg tccgcgaccg cgatcatctg
      901 cagcttgcgg cccgggctca tggcgacata gaagttgccg ttcgcatcgg gcgcaaagaa
      961 ggtcttcatg ttctgcatga aaaagaccgg gcgcaggatg acgtaggagg gaaagcccag
     1021 gctcttgatc gtctgctcga tgcgggcctt gttctcgaag tgcggcacgc ccgtcttccg
     1081 atgcgaggag ccgaccgagg tgtagaccag atgctggacg ccggccttct tggcgagctc
     1141 ggcaaactgc ttgccctgac gctcttcgcc ttcgacaccg gcttcccagg tgttctgcac
     1201 ggcaaaggcg ccccaggcac ccttgagtgc cgctgagacc gaggccgcat cgtccagatc
     1261 gcccttgacg atctccgcgc cagccgcggc gagggccttg gcggcatccc cctccggctt
     1321 gcgcgtcatc gcccgaacct gatagccggc cttgagcagt tcgcgtgcga cggcgccgcc
     1381 ctgctggccg gttgcgcctg caaccagaac tagttttcct gccattgaag aagttcctta
     1441 tcgatagttt gttgtagccg aacggacagc gggcctcagc ccgcgccgat ttctttgcta
     1501 aacccccgcg acttccttcc ggacgatggc cgcgccctcc gcgagcgccc ccagcttcat
     1561 cttcgcaacc atccgcgcca gcggcttcat gccgcagtcc gagctgagcg tcaccttgtc
     1621 ggccgggatg tgcttgagcg cgcggcggat ccggtcggcg atcttcgccg gctgctcgat
     1681 ggcgttggtg cggatgtcga gcacgccgag ttggatcccc tggttggccg gcaggttctt
     1741 caggaagtcg acgcccgcca tgtcgcgatt ggcgtagtcg agcacgaact cgtcgatgcc
     1801 ggccgtgccg aagaagtacg ggagcaccgt ctgatacccc tcggggtagt tggcgctcag
     1861 gatgtcgttg ccccacgcgt tgccgaagca gaagtgccag ccgatcttgg cgtcgacgcc
     1921 gtcgcagcac atctcgaccg actcacggat ccacttgtag tcgttcttgt caccggtgaa
     1981 gagcggcagc cacgcgccga gatcctcgaa ctggaggtac ttggcgccgg cggcgaccag
     2041 atccttcatc tccttgttga agatgggggc cagcgcgaag ctcagatcct tggcgttctt
     2101 gtagtgcttg aagtagacgt gccacgccag attggcggga cccgcgccga ccgacacctt
     2161 cacggggcgt gtcgcatgcc gagacgcgat cctgtacagg tcggccagac gaatcgggcc
     2221 gtgtgtcacg ggaccgctgt tgatgacgcc cccccagtca tcgaggatca ccttgccgga
     2281 gctgggcggc gcgttgacca ccgacggatg cggttcgcgc gatggctcga acccgccgat
     2341 gcgctcgtac atgtaccagc agaacgagcc gatgacgccg acatagtcgt cgtaccacat
     2401 gttgccgtcg gtcacgatgt cgagtccggc gctttcctga tcgcgaatga cggtggccac
     2461 ggcgtcgtcg tacgcctctt cgtgctgcac ctccttgaac gcgacccgga tgtctcggcc
     2521 aagcaactgg tgcttgaacc agtggggccg tgggtatgac ccgatcatcg tggtcgggag
     2581 aaacttgtct acgatcttcg ttgccatgta atcctctgct gttaggcgtg tcgatgtcgg
     2641 cgggtgttgg cccgcgccgg ccgtgaggcc gtgccaagcc gccgattatt gcacttgttg
     2701 gggcaatgga tctagatcac ggaacagccg tgccagtccg ctatcattgc gagggcccac
     2761 atacttccct ggagaggttg atgtccacac gagttcccgc ccgtatcgct ctggaccgct
     2821 atgcggctcg ttgcgtgaaa tttttgcccg ttgtgacccg caaagcggtc caaggcgcgc
     2881 gaagcgcgcc agaagatact ggcaaccagc caaggggctc cgcggggata tcaaaaggcg
     2941 tagccccttg gctggttctg ttcgtcgttg cggcagccgc gtgcggctcg cccgcccagc
     3001 ccgagggggg cggaacggcc gggtctccgg cgcccgcggc tccggaaccg gcggcggtga
     3061 ccctcccgat cggcgacggc accgttaccg ggctcgtcaa gttcgaaggg aaggctccag
     3121 aggcccgggt gatccggatg tcgtcggacc cgctctgcat gcccgagggg ccgacgacgt
     3181 cggaagtcgt cctggtgggc cccggcaacg ggctccagaa cgtgttcgtg tacgtgaagg
     3241 atgggctcgg cgatcgcagg ttcgccgcgc cgaagcagcc ggtcgtcctc gaccagcggg
     3301 gctgcaagta catgccccac gtgttcggcg tgcaggtcgg tcagcccgtg aacatcatta
     3361 acagcgatgc gacgctgcac aacgtgcacg cggtgccgaa ggcgaacgcc gagttcaact
     3421 tcggtcagcc gagcaagggg atggagtccg cgaagacctt caacaagccg gaaatcatgg
     3481 tgccgttccg ctgcgacgtc cacggctgga tggcggcgta cgccggagtc gtggagcacc
     3541 cgttctttgc cgtgacctca tccgacggca agtacgagat caaagggctt cccgccggca
     3601 cctacaccat cgaggcgtgg cacgagcgtt tcggcgtgca gacgcagacg gtcaaggttg
     3661 ccgacaaggg gagcgccacg gcggcgtttg cgttcaaggc gagctgaaac cggacaaccc
     3721 agcgacctgg agcttcgggt ccgtctcagg gccggaccga gaatccgagg gcggctgcgg
     3781 ccgctcggag ccggtcgtcg aaacagacga gcggtcgccc cttcgggcgc tcccggcacc
     3841 agacaagcgc agcagccagt tgggcggcgt ccgcggcgcg cacgccatgt gcatcgggga
     3901 gttcttcggc aatcgtccga agcttctcgg tcggcaggat ctcatcccac gccgtccgta
     3961 gtcgccgcag caggtgtatc gctttcactc gttcgacaga ggtgagttcc ccctctcgaa
     4021 cgagcctggc gaaggcgctg cgtgcctcga gcggtgtccc ccaccagacg accatgcgct
     4081 tcagctcgtg acgaagacgc cgaccctcag tggtcgcctg ctgcgagcaa cagaggggca
     4141 cgatcgcgct cgcgtcccag aacgccatgg tcagcgctcg tcgcgatccg cccggacgct
     4201 cgcagccgcc cgttgaagtg tgatcgttga ccggcggacc ttccagaacg atgtcggcaa
     4261 cgtgcccgag ccctttcgca tcaagccgcc cgcgaccagc gcggcgtcat cggcgtcttc
     4321 gtcatcgacg ctgaacggga cgattttcgc gatgggcctc tgtcgatcgc gcacgacgat
     4381 ctcttcaccc gcccgtactt cctggaggta ccgggtcagc ttgttcctga gatcggcgat
     4441 gttagtgctg cgcatgggac atggctatga tagccatgtc agatggttcc agcaactcgg
     4501 cgaggggcag gcgggtagtc agggcgcggc caggtaggag gcctcttcct tgagccaggc
     4561 ctcgtagtct tcctgcgtct gaatcgcgta gatgccccgc atgcggaagt ggccgaggcc
     4621 gcacagctgc gagcaggcga tgttccattc gccggtcatg gtcggtgtga accagaccgg
     4681 ctgcacgatg cccggcaccg cgtcctgctt gacccgcatc tgcggcagcc cgaagctgtg
     4741 aatgacgtcc ttgctggaga ggaagacgat gaccggcttt ccaaccggca cgttcagtcg
     4801 cccattgacc acgaggtcgt cgcgtcccgg cggttcggtc cggtcgagac cgagcgggtt
     4861 ttcaggatcc acgagccgga tgtccgtgcg gccgaacacg ccgtccgccc ccgggtagtg
     4921 gacgttccac gcgaactgct cggccacgac acgcacgatg gtgctctcgt gctccgcggg
     4981 cagcgcgtcc acgttgg
//
LOCUS       DBNGSH010000264         4586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2228275, whole genome shotgun sequence.
ACCESSION   DBNGSH010000264 DBNGSH010000000
VERSION     DBNGSH010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2228275"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="AC16CY_06085"
     CDS             complement(<1..1007)
                     /locus_tag="AC16CY_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3353387.1"
                     /translation="MEVYETLPIVPLRDVVVFPHMMMPFVIGRPSSTRALDHALLKDK
                     RIFLAAQHDASVDDPRPDDIYTMGCVANVVQSLKLPDGNIKVLVEGVDRARAVEWKED
                     KGFYRVVVKVLPKQKETSGEIESTMGRVVSLFEQYVKLSNNLQYDAMIAAVRVDDPGK
                     LADTIAAHLLVGVDEKQNLLEIISPVERLNRIAGILEIEVDKLQVDRRIQSRVKKQME
                     KAQKEYYLNEKMKAIQKELGRKDEKGSEIDELKKKIEQSRMPKDVEEKAVQELKRLEA
                     MPPMSAEATVSRNYLDWLIAVPWHKKTRENRDLKHAEKVLNEDHYGLEKIKDRILEFL
                     AV"
     gene            complement(1104..2351)
                     /gene="clpX"
                     /locus_tag="AC16CY_06090"
     CDS             complement(1104..2351)
                     /gene="clpX"
                     /locus_tag="AC16CY_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR3353388.1"
                     /translation="MVKKNGETEILRCSFCNKDQNDVRKLIAGPTVFICDECVEVCND
                     IIADDNRFENRGVRSTLPSPPEIKKFLDEYVIGQERTKRKLAVAVYNHYKRIEIQKQP
                     RSRNDVELTKSNIMLIGPTGTGKTLLAQTLAKLLSVPFTIVDATTLTEAGYVGEDVEN
                     IILKLLQAAGGDIEKCQQGIIYIDEVDKICRKDENPSITRDVSGEGVQQALLKILEGT
                     VANVPPQGGRKHPHQEFFQIDTTNILFICGGAFVGLDKVIDRRVGKKTLGFKAEVKSV
                     QKRDIGTTLEAVEPEDLIKYGLIPEFVGRLPVVGTLHELDKPALVQILTQPRNAITRQ
                     YQKLFEYENVKLRFTDDALEAIAESALSRKIGARGLRMIIEDLMLDLMYSVPGQKKLR
                     EVVVTREVVEAKDKSITLIEKAG"
     gene            complement(2482..3078)
                     /gene="clpP"
                     /locus_tag="AC16CY_06095"
     CDS             complement(2482..3078)
                     /gene="clpP"
                     /locus_tag="AC16CY_06095"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR3353389.1"
                     /translation="MSRAQLVPMVVEQTNRGERAYDIFSRLLKDSIIFIGTPIDDGIA
                     NLAIAQMLFLEAEDPDKDIQLYINSPGGSITAGLAIYDTMQFIKPNVQTICIGQAASM
                     AAVLLAAGTKGKRYSLPNSRIVIHQPLMQGLAGQATDIDIHAREILRMRERLNEILEH
                     HTGQPLKRIQDDTERDYIMSADQGKDYGIIDDVIRKRV"
     gene            complement(3105..4370)
                     /gene="tig"
                     /locus_tag="AC16CY_06100"
     CDS             complement(3105..4370)
                     /gene="tig"
                     /locus_tag="AC16CY_06100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR3353390.1"
                     /translation="MKTEFTDVSDTQKTVTIEIPSDIVDAEIDRIARGYTRQAKVPGF
                     RPGKVPATIIKKRFREQILHDVMHGLIPRAVDEALQERGIEPIDTPNIRNVAVEEGQP
                     LRFTAAIETVPPFDPGDLSTLHATKPPVAVTEDAVDQMLQRLRERAARSEPVTGRAAE
                     TGDTVVADLVRSTPQGPDRHENVSIELGAKANPPGFDAELAGLSPGDEKTFTIHFPED
                     YAVKEMAGTDVTYSATVKELRRRVLPDLDDEFAKDLGAFDSLAALRDRVRADLQAEAE
                     DNATRHVRGELMKQLAQRITFELPASLVEREIDRRLEEFARQLMQQNVDPRQAGIDWA
                     QFREAQREPARLAVSGALVLDAIARRERLEVRPEEIDTEIERFAERAGRTPAALRAQF
                     EKEGGVARLGAGLRREKAFDLALSRARML"
     gene            complement(4485..4568)
                     /locus_tag="AC16CY_06105"
     tRNA            complement(4485..4568)
                     /locus_tag="AC16CY_06105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4532..4534),aa:Leu,seq:tag)
BASE COUNT          672 a         1492 c         1465 g          957 t
ORIGIN      
        1 acggcgagga actcgaggat gcggtccttg atcttctcga gcccgtagtg gtcctcgttg
       61 agcaccttct ccgcgtgctt gaggtcgcgg ttctcgcgcg tcttcttgtg ccacggcacg
      121 gcgatcagcc agtcgaggta gttccgcgac acggtggctt cggccgacat cgggggcatg
      181 gcttccaggc gcttcagctc ctggacggcc ttctcctcga cgtccttggg catccgcgac
      241 tgctcgatct tcttcttgag ctcgtcgatc tcgctgccct tctcgtcctt gcgccccagc
      301 tctttctgga tcgccttcat tttctcgttg aggtagtact ccttctgcgc cttctccatc
      361 tgcttcttca cgcgagactg gatgcggcga tcgacctgga gcttgtccac ctcgatctcg
      421 agaatgccgg cgatgcggtt gagccgctcg acgggagaga tgatctcgag caggttctgc
      481 ttctcgtcca ctcccacgag caggtgcgcg gcaatcgtgt cggcgagctt cccggggtca
      541 tccacgcgca cggccgcgat catcgcgtcg tactgcaggt tgttcgacag cttcacgtac
      601 tgctcgaaca gcgagaccac ccgtcccatc gtcgactcga tctcgccgga cgtctctttc
      661 tgcttcggca ggaccttgac gacgacgcgg tagaagccct tgtcttcctt ccactcgacg
      721 gcgcgggccc ggtccacacc ttcgacgagc accttgatgt tgccgtcagg gagcttgagg
      781 ctctgcacga cgttggcgac gcagcccatc gtatagatgt cgtccgggcg cgggtcgtcg
      841 accgacgcgt cgtgctgcgc cgccaggaag atccgcttgt ccttcagcag ggcgtgatcg
      901 agcgcgcgcg tggaggatgg ccgcccgatc acgaacggca tcatcatgtg tgggaacaca
      961 acgacgtccc gcaggggaac gatggggagt gtctcgtaga cttccattca gggtccttgg
     1021 gaacgcggcg gctgaagccg ccgctctaca gttgccgggg cacaccccgg tggctgtagc
     1081 gcgggggctt cagcccccgc gacctatcca gccttctcga tcagcgtaat cgacttgtcc
     1141 ttcgcctcca cgacctcgcg cgtgacgacg acctcgcgca gcttcttctg cccgggcacg
     1201 gaatacatca ggtcgagcat caagtcctcg atgatcatgc gcagaccgcg ggcgccgatc
     1261 ttcctcgaca acgccgactc ggcgatcgcc tcgagcgcgt cgtcggtgaa gcggagcttg
     1321 acgttctcgt actcgaacag cttctggtac tgacgcgtga tggcgttccg gggttgcgtc
     1381 aggatctgca cgagcgccgg tttgtcgagc tcgtgcagcg tgccgacgac cggcagccgg
     1441 ccgacgaact ccgggatcag cccgtacttg atcaggtcct cgggctcgac cgcttcaagc
     1501 gtcgtcccga tgtcgcgctt ctgaacggac ttcacctcgg ccttgaagcc gagcgtcttc
     1561 ttgccgacgc gacggtcgat gaccttgtcg agcccgacga acgcgccgcc gcagatgaag
     1621 aggatgttcg tcgtgtcgat ctggaagaac tcctggtgcg ggtgcttccg tccgccctgc
     1681 ggagggacgt tggccacggt gccttcgaga atcttcagca gtgcctgctg aacgccctcg
     1741 ccggagacat cccgcgtgat ggacgggttc tcgtccttgc ggcagatctt gtcgacctcg
     1801 tcgatgtaga tgatgccctg ctggcacttc tcgatgtcgc cgccggccgc ctgcagcagc
     1861 ttcaggatga tgttctcgac gtcctcgccg acgtagcctg cctcggtgag cgtggtggcg
     1921 tctacgatcg tgaacgggac cgagagcagc ttggccagcg tctgcgcgag cagcgtcttg
     1981 cctgtgccgg tggggccgat gagcatgatg ttcgacttgg tcagctcgac gtcgttgcgg
     2041 ctccgcggct gcttctggat ctcgattcgc ttgtagtggt tgtagacggc gacggccagc
     2101 ttccgcttcg tgcgctcctg cccgatcacg tactcgtcga ggaacttctt gatctcggga
     2161 ggggagggga gcgtcgaccg gacgccgcgg ttctcgaacc ggttgtcgtc cgcgatgatg
     2221 tcgttgcaga cctcgacgca ctcgtcgcag atgaacacgg tcgggcccgc gatgagcttg
     2281 cggacgtcgt tctggtcctt gttgcagaac gagcaacgga gaatctcggt ctcgccgttc
     2341 tttttgacca tgtgtgctgt tgggctctgg cgtcgggcga ttgggtctcg ggtctgtctg
     2401 accggtgttt tcggcctcag tccggctgga ggctagagga ggcccctgat ctggagcctt
     2461 aaacctgagc ctactgcgat gttacacccg ttttcggatg acgtcatcga ttatcccgta
     2521 atcctttccc tggtcggcgg acatgatgta atcccgctcc gtatcgtcct ggatgcgctt
     2581 caggggctgc cccgtatggt gctccaggat ctcgttcagc cgctcgcgca tgcgcagaat
     2641 ttcacgcgcg tggatgtcga tgtcggtcgc ctggcccgcc aacccctgca tgagcggctg
     2701 gtggatgacg atccgggagt tcgggagcga ataccgcttc cccttcgtgc ccgccgccag
     2761 caggaccgcg gccatcgagg ccgcctgtcc gatgcagatg gtctgcacgt tcggcttgat
     2821 gaactgcatc gtatcgtaaa tcgccagccc cgcggtgatc gagccgccgg ggctgttgat
     2881 gtacagctgg atgtccttgt cgggatcctc cgcctccaga aacagcatct gggcgatcgc
     2941 caggttggcg atcccgtcgt cgatcggggt cccgatgaag atgatgctgt ccttcagcag
     3001 ccgcgaaaag atgtcgtagg cgcgttcacc gcggttggtc tgttcgacca ccatcggcac
     3061 aagctgggcg cggctcattt gtcgtgtgtg atgggcgatg ggccttaaag catcctagca
     3121 cgcgacaggg ccaagtcaaa cgccttctcc cgccgcagcc ccgcgcccag ccgggcgacg
     3181 ccgccttcct tctcgaactg ggcgcgaagc gcggcaggcg tgcggccggc ccgctcggcg
     3241 aagcgctcga tctccgtgtc gatctcctcg ggccgcacct cgagccgttc gcgccgcgcg
     3301 atcgcgtcga gaacgagcgc gcctgacacc gccagccggg ccggctcgcg ctgcgcctcc
     3361 ctgaactgag cccagtcaat accggcctgc cgcgggtcca cgttctgctg catgagttgt
     3421 ctggcgaact cctcgagccg tcgatcgatc tcgcgctcga cgagcgacgc cggcagctcg
     3481 aacgtgatcc gctgcgccag ctgtttcatc agctcgccgc gcacgtgccg cgtcgcgttg
     3541 tcctccgctt ctgcctgcag gtccgcgcgc acgcgatccc gcagcgcggc aagcgagtcg
     3601 aacgcgccga ggtctttcgc gaactcgtcg tccagatcgg gcagcacccg gcgccggagc
     3661 tccttgacgg tcgctgaata ggtgacgtcg gtgccggcca tctccttgac ggcgtagtcc
     3721 tccgggaagt ggatcgtgaa cgtcttctcg tcgcccgggg agagccccgc cagttccgcg
     3781 tcgaagccgg gaggattggc cttcgcgccg agctcgatgc tcacgttctc gtgtcgatcg
     3841 gggccctgcg gcgtgctcct gacgagatcc gcgacgacgg tgtcgccggt ctcggccgcc
     3901 cgcccggtga ccggctcgga ccgcgcggct cgctcgcgga ggcgctgcag catctggtca
     3961 acggcgtcct cggtgaccgc gaccggaggc ttcgtggcgt ggagcgtgga cagatcgccg
     4021 gggtcgaacg gcgggaccgt ctcgatggcg gccgtgaacc tgagcggctg gccctcttcg
     4081 accgcgacgt tcctgatgtt cggcgtgtcg atcggctcga tgccgcgttc ctggagcgcc
     4141 tcgtccaccg cgcgcgggat gaggccgtgc atcacgtcgt gcaggatctg ctcgcggaac
     4201 cgcttcttga tgatcgtcgc cggaactttg ccggggcgga atcccggcac cttcgcctgc
     4261 ctggtgtagc cccgggcgat gcggtcgatc tcggcgtcca cgatgtcgct cgggatctcg
     4321 atcgtgacgg tcttctgggt gtcgctgacg tcggtgaatt cggtcttcat gcggactcgg
     4381 acgcggcggc tgaagccgcc gctctacagg cgcggtacgg cgaccctgta gagcgcacgc
     4441 tttagcgtgc gcgtgagaaa tgcgattagc gtgcgcgtca gaaatgcgaa aggagggact
     4501 cgaaccccca taccctctcg ggtaccagat cctaagtctg gcgcgtctgc cagttccgcc
     4561 actttcgctc aaagccagac taacac
//
LOCUS       DBNGSH010000265        12271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3925950, whole genome shotgun sequence.
ACCESSION   DBNGSH010000265 DBNGSH010000000
VERSION     DBNGSH010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 12271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12271
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3925950"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="AC16CY_06110"
     CDS             complement(<1..929)
                     /locus_tag="AC16CY_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3353391.1"
                     /translation="MPQERSGAQIIWECLVHEGVGTVFGYPGGAILPAYDAMLDYPVR
                     HVLVRHEQGASHMADGYARAGGGVGVAMATSGPGATNLVTGIATAMMDSVPMVCITGQ
                     VPSKLIGYDAFQETDITGITLPITKHSYLVTRVEDIMPAMKEAFHLAKSGRPGPVLVD
                     ITKDAQQAKIAWEWDASPVTLRGYRPKLAVSASEMTKAVALIKASKRPVILAGQGILQ
                     SGAMREVQLFAERINAPVALTLLGLGALPASHPLHIGMMGMHGEAWVNHSIQEADLLL
                     AFGMRFDDRVTGNIKTYALNAKKIHIDIDPSEI"
     gene            1329..1841
                     /locus_tag="AC16CY_06115"
     CDS             1329..1841
                     /locus_tag="AC16CY_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353392.1"
                     /translation="MTAQAGTPLSLELTTALTTETAKVEQPVTARLRTPVVIDGITVL
                     PAGATLHGNITETERSARVKGRAHMSFRFTEVSIDGQREPLTTESLAFEAESQKGKDA
                     AKVGIGAGIGAAIGAIAGGGSGAATGAAIGAGAGVGTVLATRGQEVELAVGTELKTAL
                     ASPFGIRVEQ"
     gene            complement(1873..2244)
                     /locus_tag="AC16CY_06120"
     CDS             complement(1873..2244)
                     /locus_tag="AC16CY_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3353393.1"
                     /translation="MAKTVAVVGASGDRRKYGNKALRAFKAEGYAVVPINRREKTIEG
                     IQAYASVLDVPVPIDMATVYVQPDVALQLLEEFERKQIPEIWINPGAESDELMKEAAR
                     RRLNVICACSIVGIGRSPGEF"
     gene            complement(2266..3645)
                     /locus_tag="AC16CY_06125"
     CDS             complement(2266..3645)
                     /locus_tag="AC16CY_06125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3353394.1"
                     /translation="MVSQTVRSALRDVVGKAYVREDAESLSAYAADALGQGHPPDLVV
                     VPATTMEISAIARLCTEHRVPLVVRGAGTGYTGGAVPTQGGVVLSLERLNRILEVDET
                     NLLAVVEPNVITADLQRAVERVGLFYPPDPASLDRSSIGGNVAECAGGPRAFKYGTTK
                     RYVLALEAVLPTGEIVETGSKAVKNVVGYDLTQLLVGSEGTLAIITRVTLRLVPKPPA
                     QAALSASFRDIRGAVDAVTALIGRRVTPAALELIDRESLRALEAHVGRAMAPRGSEAM
                     LIVECDGTPAAVDEEIERVAASCRDAGATEIRRAANESERDELWRARRELSPALFATG
                     FKKINHDVVVPRGRVPQLFDAIDELSRRHRLCIASFGHAGDGNIHVNLMIDRDDADER
                     RRAREAERELFERVVALEGSITGEHGIGFAKAPYLSIELSAEEIALMKRVKHAFDPAG
                     ILNPGKIFP"
     gene            complement(3664..4872)
                     /locus_tag="AC16CY_06130"
     CDS             complement(3664..4872)
                     /locus_tag="AC16CY_06130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3353395.1"
                     /translation="MNPFARRLADVTMSATLAAAAEADRLRRAGYDVVDFSAGEPDFP
                     TPPHVKEAGKDAIDADFTRYTPAPGIPELRRAVCERYRQDYGVEITPAEVLITVGGKQ
                     ALFNAALALLDPGDDVITHAPYWPTIPEQVKLMGATPVIVHTRPEDGFAVHARAVISR
                     ITPRTKAIILNSPANPTGALIDEGELAAIADAAAARGIWIVVDLCYERLIYEPVSHNL
                     PRILLDRHRNRTVLAGSASKAYAMTGWRCGWTVAPSELTAAFNVIQGQSTSNISSITQ
                     KAALAALAGPQDAVTAMLDEYRVRRDGIHSWLTGDPGIRCVKPKGAFYLFPEISALLS
                     PDGVPTSADFARALLEKEHVVVTAGEAFDAPGYIRISYATSLEQLREGATRILRFADS
                     VRPVKAAASH"
     gene            complement(4869..5369)
                     /gene="coaD"
                     /locus_tag="AC16CY_06135"
     CDS             complement(4869..5369)
                     /gene="coaD"
                     /locus_tag="AC16CY_06135"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR3353396.1"
                     /translation="MSAGPPAGRLAIFPGSFDPITNGHVDIVLRSARLFERILVAVLV
                     NREKQALFSPDERVEIIRDVFREYPNIEVDTFDGLLVDYARRRRASAIIRGIRAVSDY
                     EYEFQMALMNRHLEPSLETVFMMPAEQYTYLSSRLIKEVFSLGADVRGLVPPAVEASM
                     RKKGLR"
     gene            complement(5366..5932)
                     /gene="rsmD"
                     /locus_tag="AC16CY_06140"
     CDS             complement(5366..5932)
                     /gene="rsmD"
                     /locus_tag="AC16CY_06140"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR3353397.1"
                     /translation="MRIIAGHLKGRRLKTPKWEGLRPTSDKLRETLFNVLSPRIEGAR
                     VLDGYAGTGALGLEALSRGAARVTFVEQDRRAAALIEENATICGVEGRYTIACVDFLA
                     APRLDAEGPFDLILLDPPYDEGRVRDALDRAGRLLSAGGLLVLERATRQDPDIPPTLV
                     RVRDVASGDSTLTMFTTTAGHTIASERS"
     gene            complement(5936..8023)
                     /gene="recG"
                     /locus_tag="AC16CY_06145"
     CDS             complement(5936..8023)
                     /gene="recG"
                     /locus_tag="AC16CY_06145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3353398.1"
                     /translation="MGVDFLTGPLQFLKGVGPRKAADLKRAGLVTVEDLLYRLPFRYE
                     DRSRMQPIATLRPGVKAAVLGEIKAAHLTVTRRRGFRIFNAVVADASGSIRCTWMNQA
                     FLTDILKPRLQVVVFGDVKLDSSGLHFMNPEYELVTEDLSSVHTGRVVPFYEKTGTVT
                     PNMQRRLVRQVLDQIPDEIRDPLPEDLRSRLRLVPRRAALEQSHFPSNDASVEDLNSF
                     RTPAQRRMIFEEFFLFQLGHAWRRQSSASERKPWVPAVDDRVRASAARVLPFKLTPGQ
                     RHAVKEIVDDMQLPAPMHRLLQGDVGAGKTIVALLSAVVAMENGLQVAFMAPTEILAV
                     QHFASIAKLLSSSRFRVDLLTGSTPGLQKHTVTSHLERGTTHLVVGTHALVQDSVRFH
                     RLGLVIIDEQHRFGVEQRALLRTKGLRPDVLLMTATPIPRTLALTDYSELDVSKITDL
                     PPGRKPVRTLVKPESRRDEVFELMRRQLDEGRQAYVIYPLVEESEKIDLKSATEMADH
                     LQAEVFPAYRVALLHGRMKQDAKDHVMQAFVSGQVQVLVSTTVVEVGVDVPNASVMVV
                     EHAERFGLSQLHQLRGRVGRGSWDSYCVLLYQAPWTDDARERLHTMATTNDGFVIAER
                     DLELRGPGDVFGTRQSGLPKLRTGDLVRDKDIMEMAHDEARKLVAGGGLTRELLSYVQ
                     QRWQEQFGLIEVG"
     gene            complement(8032..8634)
                     /locus_tag="AC16CY_06150"
     CDS             complement(8032..8634)
                     /locus_tag="AC16CY_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353399.1"
                     /translation="MSLRLRRCSVLVACVLGLTFVHAAAISKQQADDFDRKVMLVNRQ
                     ATGDEAPGLRRTSFSEAEVNSWFAYGGREVLPVGVVSPKVTILADGLVKAEAIVDLEA
                     IARRRSTGRTLDPWSYLGGRVPVTASGILRTQNGKGRFDLEQAAVSGVPIPKAVLQDM
                     VTYYSRTADAPAGVRLDEPFQLPARIRQIELKQGQAVVVQ"
     gene            8773..9378
                     /gene="lexA"
                     /locus_tag="AC16CY_06155"
     CDS             8773..9378
                     /gene="lexA"
                     /locus_tag="AC16CY_06155"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR3353400.1"
                     /translation="MLPLTKRQREILDYLNDFIQQHGYAPSLEEIGRRFSLSSLATVH
                     KHLSNLQEKGFIRRAWNRSRSVELVPTRSAGRALELPLLGYVAAGAPIEAVAGSETIS
                     VPETLAGKRDSYVLRVKGDSMIDEQIRDGDFVIVEDRKSADNGEMVIALVGGLEVTLK
                     KFYRENGRVRLQPANPAMQPIMVDADHVQVQGVVVGVMRKY"
     gene            9474..9800
                     /locus_tag="AC16CY_06160"
     CDS             9474..9800
                     /locus_tag="AC16CY_06160"
                     /inference="COORDINATES: protein motif:HMM:NF023376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR3353401.1"
                     /translation="MSDQPERKQINFTIVPDERPGEPRTYANFCSIAHTPFDFTLTFC
                     EVMPLSEKEVREAEQEHIVRAPVRARVVVPVQFVPNLIAALQDHMRVFSETQSNAGWS
                     KGGPIH"
     gene            9797..10513
                     /gene="nth"
                     /locus_tag="AC16CY_06165"
     CDS             9797..10513
                     /gene="nth"
                     /locus_tag="AC16CY_06165"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR3353402.1"
                     /translation="MKPPQTVRRVIELLSEQHPNADTELHHRNAFELLVATILSAQST
                     DARVNVVTPGLFAKYPDARALASARPSALERQIVSTGFFRQKAKALIGMARTLVAEHA
                     GEVPASMDALTKLPGVGRKTANVVLGHALGVPGLPVDRHVLRVSNRIGIAEGDDPVQV
                     EAQLCAALPPEMWTRASDVQILHGRRVCRPKPMCESCLVRDDCDYYHHVVGKEPPQAR
                     APKRARARSRDPRKVRTPAR"
     gene            10479..10883
                     /locus_tag="AC16CY_06170"
     CDS             10479..10883
                     /locus_tag="AC16CY_06170"
                     /inference="COORDINATES: protein motif:HMM:NF018021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR3353403.1"
                     /translation="MTRARFERLLAEAITLIPGRFRREMKNLALVVEDEPSLDLLEEM
                     EIEPPDSLYGLYQGTPLPERTWGYGNQLPDRITLFQRPIEEDCEDEDEVRAVIGETLI
                     HEVGHYFGLSEEEIEEIEEKYWRGDTQDEDDA"
     gene            10880..11704
                     /gene="rsmA"
                     /locus_tag="AC16CY_06175"
     CDS             10880..11704
                     /gene="rsmA"
                     /locus_tag="AC16CY_06175"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR3353404.1"
                     /translation="MRPRTFPAARKRFGQHFLEPAWVARIVDAMEPLPDQTFLEIGPG
                     RGAMTRPLAERAARVIAVEIDRDLAAALPLTLPPNVRVVQADFLDLDLAGLLHDEALP
                     IRVVANLPYNVATPILFRILHEAGDGRVLSDATLMLQREVADRLLAGPGTSDYGALTI
                     QVRLLADVSPVLKLPPGAFRPAPKVHSAVVKLRFRPPAVDVGDRAAFERLVRGLFLQR
                     RKTLLNALKPVADAFGSLAPEVIERAGVDGRRRPETLSLEEMARLTRCAIVFAGNH"
     gene            11881..>12271
                     /locus_tag="AC16CY_06180"
     CDS             11881..>12271
                     /locus_tag="AC16CY_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353405.1"
                     /translation="MSQSAGSDASATLEVVALGGLGEFGMNMMAVSWQDTTILIDAGV
                     MFPEPDQLGVDLIVPDMTFVESRRENVKALVLTHAHEDHIGAVPHVLPRFDGPVYGTA
                     LTLAFVEPKLQEHGIDARDRFQVVAPRQ"
BASE COUNT         2067 a         4150 c         3996 g         2058 t
ORIGIN      
        1 ttgatctcgg agggatcgat gtcgatgtgg atcttcttcg cgttcagggc gtaggtcttg
       61 atgttgccgg tcacccgatc gtcgaagcgc atgccgaagg cgagcagcag gtccgcttcc
      121 tggatcgagt ggttcaccca ggcttcgccg tgcatcccca tcatgccgat gtgaagcgga
      181 tgactcgccg gcagcgcgcc gaggccaagg agcgtcaggg caaccggcgc gttgatccgc
      241 tcggcgaaca actgtacttc ccgcatcgca ccgctctgta gaatgccctg ccccgccagg
      301 atcaccggcc tcttcgatgc cttgatcagg gcaaccgcct tcgtcatttc tgaggccgag
      361 accgccagct tgggacggta gcctcggagc gtcaccggcg acgcatccca ttcccaggcg
      421 atcttcgcct gctgcgcgtc cttcgtgatg tccacgagca ccggacccgg ccgtcctgac
      481 ttggccaggt gaaaggcctc cttcatcgcg ggcatgatgt cttcgacgcg cgtcaccagg
      541 taactgtgct tggtgatcgg cagcgtgatg ccggtgatgt cggtctcctg gaaggcgtcg
      601 tagccaatca atttgctcgg cacctgcccg gtgatgcaca ccatgggcac cgaatccatc
      661 atggcggtcg cgatgcctgt caccaggttc gtcgcgccgg ggcccgaggt cgccatcgcc
      721 acaccgacgc ccccgccggc gcgggcgtac ccgtccgcca tgtgcgacgc accctgctcg
      781 tgccggacga gcacgtgcct caccgggtag tcgagcatcg cgtcgtacgc cgggaggatc
      841 gcgccgcccg gatacccgaa cacggtgccg acgccctcgt gaacgagaca ctcccagatg
      901 atctgtgcgc cgcttcgctc ctgtggcatg gaccccagtg tgcactgcgg cggtacatca
      961 gtcaaattga tagtaattcg gacatgataa cgacatgtga tacagaccgg cctatggatg
     1021 gaacttgacc tcgagcctcg acagactgat ccttgagcac ccccgcaacg cgagaggatt
     1081 cgaccatgag atacccccgc atgtcagccg ctcttctcac cgctgcactc gcgctcgcgt
     1141 gtgccggagc cccgcagaac gctgagaatg ctcccgcgcc cgaggccgcg cccgctgctg
     1201 aaccggccca gcaagccgat gctccggcag ccgcgcgtac gccgcgaccc gcggcgccac
     1261 gttccgcgag tcctgagccg agcaggccgg cggcacctcc gcccccaccg gcgagacgtt
     1321 ttcgcgaggt gacggcgcaa gcgggtactc cgctctctct ggaactcacc accgcgctga
     1381 cgaccgaaac cgcgaaggtc gagcagccgg tcaccgcgag gctacgtacc ccggtcgtca
     1441 tcgacgggat caccgttctg ccggcgggtg cgaccctcca cgggaacatt accgagaccg
     1501 agcgttcggc gcgcgtgaaa ggccgcgcgc acatgtcgtt ccgttttacg gaggtgtcca
     1561 tcgacggaca acgcgagcca ctgaccaccg agtcgctcgc attcgaggct gaatcccaga
     1621 aaggcaagga tgccgccaag gtcggcatcg gcgcaggtat cggcgcggcg atcggcgcca
     1681 tcgccggcgg cggcagcggc gcggcgactg gcgcggccat cggggcaggc gccggagtcg
     1741 gcacggtgct cgccacgcgt ggtcaggaag tcgagctcgc ggtcggcacc gaactgaaga
     1801 cggcgctcgc gtcgccgttc ggaattcgcg tggaacagta agtcttgagc cttgagtgtt
     1861 gaatgttgag tttcaaaact cacccgggct ccggccgatc ccgacgatgc tgcaggcgca
     1921 gatgacgttc aggcgtcgtc gggcggcttc cttcatcaat tcgtcgctct ccgctccggg
     1981 gttgatccag atctccggga tctgtttgcg ctcgaattcc tcgagcagct gaagcgccac
     2041 gtccggctgc acgtacaccg tagccatgtc gatggggacc ggcacgtcca gcaccgatgc
     2101 gtacgcctga atcccttcga tagtcttctc ccgtcggttg atcggcacaa ccgcgtagcc
     2161 ttcggccttg aaagccctga gcgccttgtt gccgtacttg cgtcgatcgc ccgaggcacc
     2221 gacgacggcc accgttttcg ccatggtcgg aattatccct ggaagctacg gaaagatttt
     2281 gccagggttg agaatgccgg cgggatcgaa cgcgtgcttg acgcgcttca tgagcgcaat
     2341 ctcttcggcc gacagctcga tggacaggta aggggccttc gcgaatccga tcccgtgttc
     2401 gcccgtgatc gagccttcga gggcgaccac gcgctcgaag agctcgcgct ccgcttctcg
     2461 cgcccgccgc cgctcgtcgg cgtcgtcgcg atcgatcatc agattgacgt ggatgttgcc
     2521 gtctcctgcg tggccgaagg aggcgatgca gagccggtgc cgcctggaca gttcgtcgat
     2581 ggcatcgaac aactgcggca cgcggccgcg cggcacgacg acgtcgtggt tgatcttctt
     2641 gaacccggtg gcaaagagcg ccggcgacag ctcacgtctg gcgcgccaga gctcgtcgcg
     2701 ctcgctctcg tttgcggcgc ggcgaatctc ggtggctccc gcgtcgcggc acgaagcggc
     2761 gacgcgttcg atctcttcgt ccacggccgc cggcgtgccg tcgcactcga cgatcagcat
     2821 cgcctccgag ccgcggggcg ccatggcgcg gccgacgtgc gcctccaggg cgcgcagcga
     2881 ttcccggtcg atcagctcga gagccgctgg tgtcactctc cgtccgatga gcgcggtcac
     2941 cgcatcgacg gcgccgcgga tgtcccggaa cgaggctgac agcgccgcct gtgcgggcgg
     3001 tttcgggacg agccgcagcg tcacgcgggt aatgatggcc agagtccctt cggatccgac
     3061 gagcagctgc gtcagatcgt agccaacgac gttcttcacg gctttcgagc cagtctcgac
     3121 gatctccccc gtgggaagca cggcttcgag cgcgagcacg tagcgcttgg tcgtgccgta
     3181 cttgaacgcc cgcggccccc cggcgcactc ggctacgttc ccgccgatgg acgagcggtc
     3241 aaggctggcg ggatcgggcg gatagaacag ccccacgcgc tcgacggcgc gctgaaggtc
     3301 ggcggtgatc acgttcggct cgacgacggc cagcaggttc gtctcgtcca cctccaggat
     3361 ccggttcagc cgctcgagcg acagcacgac gcccccctga gtgggtaccg cgccgccggt
     3421 gtagccggta ccggcgccgc gcacgaccag cgggacgcga tgttcggtgc agagacgcgc
     3481 gatggccgaa atctccatcg ttgtcgccgg gaccacgacg agatccgggg gatggccttg
     3541 accgagcgcg tcggcggcat acgcggagag cgactcggcg tcctcccgga cgtacgcctt
     3601 gccgacgacg tctcgaagcg ccgagcgaac cgtctgggac accatcgacc gccggccttt
     3661 cggtcagtgc gacgccgcgg ccttcacagg gcgcacggag tcggcgaatc gcaggatgcg
     3721 agtggcgcct tcgcggagct gctccagaga cgtcgcgtac gagatccgga tgtatcccgg
     3781 cgcgtcgaac gcttcgcccg cagtgacgac cacgtgctct ttctcgagca gcgccctggc
     3841 gaagtcggcc gacgtgggca cgccgtccgg cgagagcagc gcggagatct ccggaaagag
     3901 atagaacgcg cccttcggct tgacgcagcg gatcccaggg tccccggtca gccaggaatg
     3961 gattccgtcc cggcggactc ggtactcgtc gagcattgcc gtgaccgcat cctgcggacc
     4021 ggcaagcgcc gccagcgcgg ccttctgtgt gatggagctg atattcgacg tggactgacc
     4081 ctggatcacg ttgaacgccg cggtgagctc cgacggcgcc acggtccagc cgcagcgcca
     4141 cccggtcatc gcgtaagcct tcgacgccga gcccgccagc accgtacggt tgcggtgccg
     4201 gtcgagcaag atcctcggaa ggttgtgcga caccggctcg tagatcaggc gctcgtagca
     4261 caaatcgacg acgatccaga tgccgcgcgc ggcggccgcg tcggcgatcg ccgcaagctc
     4321 gccctcgtcg atcagcgcac ctgtcgggtt ggccggcgag ttgaggatga tcgccttcgt
     4381 cctcggcgtg atgcgggaga tgacggcccg cgcgtgcacg gcgaatccgt cttcgggccg
     4441 cgtgtgcacg atcaccggcg tcgcgcccat cagcttgacc tgttcgggaa tcgtcggcca
     4501 gtacggcgcg tgcgtgatga cgtcgtcacc cggatcgagg agcgcgagcg cggcattgaa
     4561 gagcgcctgc ttgcctccca ccgtgatcag gacctcggct ggcgtgatct ccacgccgta
     4621 gtcctgccgg taacgctcgc acacggcccg gcggagttcc ggaatgccgg gcgcgggcgt
     4681 gtaacgggtg aagtcggcgt cgatcgcgtc cttgccggcc tccttcacgt gcggcggcgt
     4741 cggaaaatcg ggctcgccgg cgctgaagtc caccacgtcg tagcccgccc tgcggagccg
     4801 gtcggcttca gcggctgccg ccagcgttgc cgacatcgtc acgtcggcaa ggcgccgggc
     4861 gaagggattc accgcagacc tttctttctc atcgatgcct cgaccgcggg cggcacaagg
     4921 ccgcgcacgt ccgcgcccaa gctgaagact tccttgatca ggcgcgagct gaggtaggtg
     4981 tactgctcgg ccggcatcat gaagaccgtc tcgaggctgg gctccaggtg ccggttcatg
     5041 agcgccatct ggaattcgta ctcgtagtcc gacacggcgc ggatgccccg gatgatggcg
     5101 ctcgcgcggc gtcgccgcgc gtagtccacg agcagcccgt cgaaggtatc gacctcgatg
     5161 ttcgggtact cgcgaaacac gtcgcggata atctccacgc gctcgtccgg cgagaagagc
     5221 gcctgctttt cgcggttcac cagcacggcc acgaggatgc gctcgaagag acgggcgctg
     5281 cggaggacga tgtccacgtg gccgttggtg atcgggtcga acgagcctgg aaagatggcg
     5341 aggcggccgg ctggtgggcc cgcgctcatg agcgctccga ggcaatcgtg tggccggccg
     5401 tcgtcgtgaa catggtcaga gtgctgtcac cggatgcaac gtcacggact cgcacgagcg
     5461 tcggcggaat gtccggatcc tggcgcgtgg cgcgctcgag cacgagcagc ccccccgccg
     5521 acagcaggcg tcccgcccgg tcgagcgcgt cacggacgcg gccctcgtcg taaggcggat
     5581 ccagcaggat cagatcgaac gggccctccg catcgagacg cggcgcggcg agaaagtcga
     5641 cgcaggcgat agtatatcgc ccctccacgc cgcagatggt cgcattctcc tcgatgagcg
     5701 cggccgcgcg tcgatcctgc tcgacgaagg tgacgcgggc ggctccgcga ctcagcgcct
     5761 cgagacccag cgcgccggta ccggcgtaac catccagcac gcgcgcgccc tcgatacgcg
     5821 gagacaggac gttgaacagc gtctcccgca gcttgtccga ggtcggccgc agtccctccc
     5881 acttgggcgt cttgaggcgg cgacccttca ggtggcctgc aatgatccgc acgcctcagc
     5941 caacctcgat caacccgaac tgctcctgcc agcgttgctg gacgtacgac aggagctcgc
     6001 gcgtcaggcc gccacccgcc acgagcttgc gcgcctcatc gtgcgccatc tccatgatgt
     6061 ctttgtcgcg cacgagatcg cccgttcgga gcttcggaag gccggactgg cgtgtcccga
     6121 agacgtcgcc gggaccgcgc agctccagat cgcgctccgc gatgacgaac ccgtcgtttg
     6181 tggtggccat cgtgtgaagg cgctctcggg cgtcgtcggt ccagggagcc tggtacagca
     6241 gcacgcagta cgagtcccac gacccccgtc cgacgcgccc gcgaagctga tggagctggg
     6301 acaggccgaa acgctccgca tgttcgacga ccatgaccga cgcgttcggg acgtccactc
     6361 ccacttcgac cacggtggtc gagacgagca cctggacctg gcccgacacg aacgcctgca
     6421 tgacgtggtc tttcgcgtcc tgtttcatcc gcccgtgcag cagcgccacg cggtaggcgg
     6481 gaaacacttc agcctgcagg tggtcggcca tctcggtggc cgacttgagg tcgatcttct
     6541 cggactcctc gacgagcgga tagatgacat acgcctgccg gccctcgtcg agctggcggc
     6601 gcatcagctc gaagacctcg tcacggcgcg actcgggttt gaccagcgtg cgtaccggct
     6661 tgcggcccgg cggcaggtcg gtgatcttcg agacgtcgag ctcgctgtag tcggtcagcg
     6721 cgagcgtgcg cggtatcggc gtcgccgtca tgagcaggac gtctggtcga aggcccttgg
     6781 ttcgaagcag ggcccgctgc tccaccccga accggtgctg ttcgtcgatg atgacgagtc
     6841 cgagcctgtg aaacctcacc gagtcctgca cgagcgcgtg cgtgcccacc acgaggtgcg
     6901 tcgtcccgcg ctcgagatgg gacgtgaccg tgtgcttctg cagccccggc gtgctgcccg
     6961 tcagcagatc gacacggaac cgcgacgacg acagcagctt cgcgatgctg gcgaagtgct
     7021 ggacggccag gatttccgtc ggcgccatga aggcgacctg cagcccgttc tccatcgcca
     7081 cgacggccga gagcagcgcg acgatcgtct tgccggcgcc cacatcgccc tgaagaagcc
     7141 ggtgcatcgg ggccggcagc tgcatgtcgt ccacgatttc cttgacggcg tgccgctgcc
     7201 ccggggtcag cttgaacggc agcacgcgcg ccgccgacgc acgaacgcga tcgtccacgg
     7261 ccggcaccca gggcttgcgc tcggacgcgc tcgactgccg ccgccaggca tgccccagct
     7321 ggaagaggaa gaactcctcg aaaatcatgc gccgctgagc cggggtgcgg aacgagttga
     7381 gatcctcgac cgaggcgtcg ttggagggaa agtgcgactg ttcgagggcc gcgcggcgcg
     7441 ggaccagccg cagccgcgag cgcaggtcct cgggcagtgg atcccgaatc tcgtccggga
     7501 tctggtcgag cacctgccgc acgagccgcc tctgcatgtt gggggtgacc gtgccggtct
     7561 tttcgtagaa gggcaccacg cggccggtgt ggacgctcga cagatcctcg gtcacgagct
     7621 cgtactcggg gttcatgaaa tggaggccgc tcgaatccag tttcacgtcc ccgaacacga
     7681 ccacctgcag gcgcggtttg agaatgtctg tcaggaacgc ctggttcatc caggtacagc
     7741 ggatcgatcc gctcgcgtcg gccacgacgg cgttgaagat acgaaaaccc cgccggcgcg
     7801 tgactgtgag gtgcgcggct ttgatctcgc caaggaccgc cgccttcacc ccgggacgga
     7861 gcgtagcgat cggctgcatc cggctgcgat cctcatagcg gaacggcagc cggtacagga
     7921 ggtcttcgac cgtgacgagc ccggctcgtt tcaggtccgc agcctttcgc ggaccaacgc
     7981 ctttcaggaa ttgcagcgga ccggtgagga agtcaacgcc caatcggagg gttactggac
     8041 gaccaccgcc tgcccttgtt tgagctcgat ctgacgaatc ctcgccggaa gctggaacgg
     8101 ttcatcgagg cgcacgcctg cgggagcgtc cgccgtccgc gagtagtacg tcaccatgtc
     8161 ctgaaggacg gccttgggaa tcggtacgcc cgacacggcg gcctgctcga gatcgaagcg
     8221 ccccttgccg ttctgcgtgc gcaggatgcc gcttgccgtc accggaaccc tgccccccag
     8281 gtagctccat ggatccaggg tccgtccggt ggaccggcgc ctggcgatcg cttccaggtc
     8341 cacgatcgcc tcggccttca ccaaaccgtc ggcgaggatg gtcaccttcg gcgacacgac
     8401 accgaccggg aggacctccc ttccgccata cgcgaaccac gagttgacct cggcttccga
     8461 gaacgacgtg cgacggagtc cgggtgcttc gtcccccgtg gcttgccggt tgaccagcat
     8521 caccttgcgg tcgaagtcgt ccgcctgttg cttggagatg gcggccgcat gcacgaacgt
     8581 gagcccgagg acgcacgcga cgagcactga gcagcggcga aggcggagag acatgggccg
     8641 agcctcctca aagagctggc tgcggatacg agtgtagcac ccgccttgat tttcgcttta
     8701 tgttcgcata tagtcgtcgg cgattccagc ccctcggaat cgacgttaac cggtcgtccc
     8761 gcggaggatg ccgtgctccc cctcacaaaa cgccagcgcg agatcctgga ttacctcaac
     8821 gacttcatcc agcaacacgg ctacgcgccc agcctcgagg agatcggccg ccgcttcagc
     8881 ctgtcgtccc tcgcgacggt ccataagcat ctgagcaacc tgcaggaaaa agggttcatc
     8941 cgccgcgcct ggaatcggag ccggtcggtc gaattggtgc cgacacggag cgcggggcgg
     9001 gcgctggagc tcccgcttct cgggtacgtc gccgccgggg cgccgattga ggccgtagcc
     9061 gggagcgaaa cgatttccgt gcccgaaacc ctggccggca agcgggacag ctacgtcctc
     9121 cgggtgaagg gcgactcgat gatcgacgag cagattcgcg acggtgattt cgtgatcgtc
     9181 gaggaccgca agtccgccga caacggcgaa atggtgatag ccctggttgg cggcctcgag
     9241 gtcacgctca agaagttcta ccgcgaaaac gggcgcgtcc gcctccaacc ggccaatccc
     9301 gcgatgcagc caatcatggt ggacgccgac cacgtgcagg tccagggcgt cgtcgtcggc
     9361 gtcatgcgca agtactgacg tcagcaaaca cgaccctgat tcctgattcc tgattcctga
     9421 ttcctgattc ctgacccctg ccccctaacc cctgtcccct gactccttgt cttatgtctg
     9481 accagcccga gcgcaagcaa atcaacttca ccatcgtccc cgacgagcgg cccggtgagc
     9541 cgcgcacgta cgccaatttc tgctccatcg cccacacccc cttcgacttc acgctcacct
     9601 tctgcgaggt gatgccgctg tcggagaagg aggtgcggga ggccgagcag gaacacatcg
     9661 ttcgggcgcc cgttcgcgcc cgcgtggtcg tgccagtcca gttcgtgccg aacctgatcg
     9721 cggcgctgca ggaccacatg cgtgtcttct cggagacgca gtcgaacgcg ggctggtcga
     9781 agggggggcc gattcactga agcctccgca gaccgtccgc cgcgtaatcg agctgctgtc
     9841 agagcagcat ccaaacgccg atacagagct tcaccatcgc aatgcgttcg agctgctggt
     9901 cgcgacgatc ctgtccgcgc aatcgaccga tgcgcgcgtg aacgtcgtca cgccagggct
     9961 tttcgcgaag tatcccgatg cccgcgcgct cgccagtgca cgcccgagtg cgctcgaaag
    10021 acaaatcgtg tccaccggct tctttcggca gaaggccaaa gcgctgatcg ggatggcccg
    10081 aacgctcgtg gccgagcacg ccggggaggt cccggcgagc atggacgcgc tcacgaagct
    10141 gccgggagtc gggcgcaaga cggcgaacgt ggtgctgggc cacgcgctcg gcgtgccggg
    10201 tcttcccgtc gaccgccacg tccttcgcgt ctcgaaccgc atcggcattg ccgagggcga
    10261 cgaccccgtg caggtcgagg cacagctctg cgccgcgctc ccgcccgaga tgtggacgcg
    10321 cgcgtccgac gtccagatcc tgcacggccg ccgcgtctgc cggccaaagc cgatgtgcga
    10381 gagctgcctc gtccgcgatg actgcgacta ctatcaccac gtggtcggca aggagccgcc
    10441 acaggcacgc gccccgaagc gcgctcgagc caggagccgt gacccgcgca aggttcgaac
    10501 gcctgctcgc tgaagccatc acgctcatcc ccggccggtt ccgccgggag atgaagaatc
    10561 tcgcgctcgt cgtggaggac gaacccagcc tcgacctgct cgaggagatg gaaatcgagc
    10621 cgccggactc gctgtacggc ctttaccagg gcacgccgtt gcccgagaga acctgggggt
    10681 acggcaacca gcttcccgat cgcattaccc tgtttcagcg gccgattgaa gaggactgcg
    10741 aggacgaaga cgaggtccgg gccgtcatcg gcgagacctt gattcacgag gtcggacact
    10801 acttcggcct cagcgaggaa gagatcgagg aaatcgaaga gaagtactgg cgtggagaca
    10861 cgcaggacga ggacgacgct tgaggccgcg aacgtttcct gcggcgcgga agcgattcgg
    10921 acagcacttt ctcgagccgg cctgggtggc caggatcgtc gacgcgatgg agccgctgcc
    10981 cgaccagacg ttcctggaga tcggcccggg gcgcggcgcc atgacccgac cgctggccga
    11041 gagagcggcg cgcgtcatcg ccgtcgagat cgatcgggat cttgcggcgg cactacccct
    11101 cacgcttccc ccgaacgtcc gcgtcgtcca ggcggatttt ctggacctcg accttgcggg
    11161 cctgcttcac gacgaggcgc tcccaatccg cgtcgttgcc aacctcccgt acaacgtcgc
    11221 gacgccgatt ctctttcgca tccttcacga ggccggagac ggccgagtcc tgagcgatgc
    11281 gaccctgatg ctccagcgcg aagtcgccga tcgcttgctg gccgggcctg gcacctcgga
    11341 ctacggcgcc ctgacgattc aggtccggct gcttgcggac gtcagtccgg tcctgaagct
    11401 gccgcccggc gcgttccggc ctgcgcccaa ggtgcactcg gcggtcgtga agttgcgctt
    11461 tcgtccgccc gcggtcgacg tcggtgaccg ggcagccttc gagcggctcg tccgggggct
    11521 ctttctgcag cgccgaaaaa cattgctgaa cgcactcaag ccggtcgccg acgccttcgg
    11581 gagcttagcg cctgaagtga ttgagcgggc tggggttgac ggccggcggc ggcccgagac
    11641 gctgtcgctg gaagagatgg cccggctgac gcgctgtgct atagttttcg ccggaaacca
    11701 ctgaacccgc ccgggtttta ccccttcgcg ccgctctggt tccctgagcg ggacgaaggc
    11761 caggcgtacc cagccgcctg aacgctcgga gtgtcaacac cacgccgagg ctcccgcgag
    11821 tcgcacccgc cagggttcgg cgccggcggc aggcacctca acgcttcgac tgggccttca
    11881 atgagtcagt ccgcaggatc ggacgcgtcg gcgacgctcg aggtcgttgc cctcggcggt
    11941 ctcggcgaat tcggcatgaa catgatggca gtgtcctggc aggacacgac catcctgatc
    12001 gatgccggcg tcatgtttcc agagccggac cagcttggcg tggacctgat cgtgcccgac
    12061 atgacgtttg tcgagtcccg ccgcgagaac gtgaaggcgc tcgtcctgac ccacgcccac
    12121 gaggaccaca tcggcgccgt gccccacgtg ctgccccgat tcgacggacc tgtgtacgga
    12181 acggcgctca cgctcgcttt cgtcgagccc aagctccagg agcacggcat cgacgcgcga
    12241 gaccggtttc aggtggtcgc gccacggcag c
//
LOCUS       DBNGSH010000266         4173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1888764, whole genome shotgun sequence.
ACCESSION   DBNGSH010000266 DBNGSH010000000
VERSION     DBNGSH010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1888764"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /gene="atpA"
                     /locus_tag="AC16CY_06185"
     CDS             complement(<1..1261)
                     /gene="atpA"
                     /locus_tag="AC16CY_06185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3353406.1"
                     /translation="MANLIQDIEAQIAGAKTTALKRSIGIIREIGDGVARVEGLGDVM
                     LNEMLDLGNGITGIALSLEETEVGVIILGDYTSLEEGNEVTATGKLLQVPVGKGLLGR
                     VVNMLGEPVDGKGPIASSEAYPVEKIAPGIMRRKPVSQPVQTGIMAIDAMIPIGRGQR
                     ELIIGDRSTGKTTICIDTIISQGRLNREAEAAGNKDYRPLYSIYVAIGQKQSTIARIV
                     AVLEEAGAMPYTIIIASPAADSATNQYLAPFAGAAMGEWFMDHGMDALIVYDDLSKHA
                     VAYRQVSLVLKRPSGREAYPGDVFYLHSRLLERAARVGEQYGNGSLTALPIIETQAGD
                     VSAYIPTNVISITDGQIYLETDLFYQGIRPAISVGLSVSRVGSAAQLKAMKQVAGRLK
                     GDLAQFRELAAFAQFGSDLDATTQSRID"
     gene            complement(1294..1689)
                     /locus_tag="AC16CY_06190"
     CDS             complement(1294..1689)
                     /locus_tag="AC16CY_06190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR3353407.1"
                     /translation="MKTRRQIQRDAKRLYRACLVNGALDEGRVRGIVQRVAGGGGPAA
                     LAVLTRFQRLVRLDRAAHSAKVESATPLPDDVRASIEGSLTRIHGRGLTTSFSGNAAL
                     LGGVRVTVGSDVYDGSIQGRLAALEAKFQ"
     gene            complement(1686..2228)
                     /gene="atpF"
                     /locus_tag="AC16CY_06195"
     CDS             complement(1686..2228)
                     /gene="atpF"
                     /locus_tag="AC16CY_06195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR3353408.1"
                     /translation="MTILALMMQEQGGGQVEQIARTFGVNWPHLTAQIISFSIVCALL
                     YWLAYKPVLQMLETRRKQIAQGLENTEKINAALAGIEAQRQGVIAEAQAQSTKLVAEA
                     RDLAKKLSEQETQRAVAAAEQILVKAREAAEQEHARMLAELRREVGRLVVQTTTAVVG
                     KVLTSDDERRLAEETARQLQ"
     gene            complement(2254..2445)
                     /locus_tag="AC16CY_06200"
     CDS             complement(2254..2445)
                     /locus_tag="AC16CY_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR3353409.1"
                     /translation="MGNLHVGLAALGAALGVGLIGMKASEAVGRNPGAATAILVQSIL
                     SIAFAEAIVFYALFLVQAQ"
     gene            complement(2524..3396)
                     /gene="atpB"
                     /locus_tag="AC16CY_06205"
     CDS             complement(2524..3396)
                     /gene="atpB"
                     /locus_tag="AC16CY_06205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3353410.1"
                     /translation="MARILLTVLAIGLNAAPLLAVGEEGHHGLPQSAVEIARPFGFPV
                     TNSMVVTWIVGLALIAFARIATRNMKQVPTGSQNFLEWLVEGLYTFLEGIIGPLVKRT
                     FWFFATVFIFILASNWFGLIPGVGSMGWGHQGEHGFVLEEPFFRGANADVNMTLAMAL
                     IFFVMWVVWALREVGPLGMAKELFAPKGSNTGFLKVLMIVVFLAAGLLEVVSILFRPV
                     SLSFRLYGNIFAGENMLETMAAVPNIGWLVQIPFYFLELLVGLVQALVFMLLTAVFTL
                     LICQHHEEGSAAHG"
     gene            complement(3473..4117)
                     /locus_tag="AC16CY_06210"
     CDS             complement(3473..4117)
                     /locus_tag="AC16CY_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3353411.1"
                     /translation="MAASERILDPIERTTEVMFGIIMVLTFTSSISVVEGGEAENRTI
                     LLGAIGCNLAWGLIDAVFYLLANFTQRARGLEILRAVRGARTDEAQRLLLDALPSPVA
                     EVLTPDDIDALRERLRHQPEPPARVAVEGRVLKGAFGVFLLVFLSTFPVVIPFLVVPQ
                     PALALRTSNAVALVMLFVTGWSLGKYARRPAWLVGLGTVGLGVILVAITIALGG"
BASE COUNT          722 a         1433 c         1312 g          706 t
ORIGIN      
        1 gatcgattct cgactgcgtc gtggcgtcga gatccgaccc gaactgcgcg aaggcggcca
       61 gctcgcggaa ctgcgccagg tcgcccttca gccggccggc gacctgcttc atcgccttga
      121 gctgcgcggc cgacccgacg cgcgacaccg acaagccgac cgagatcgcc gggcggatgc
      181 cctgatagaa caggtccgtc tcgaggtaga tctggccgtc ggtgatggag atgacgttgg
      241 tcggaatgta cgccgagacg tcgccggcct gcgtctcgat gatgggaagc gcggtcagcg
      301 agccgttgcc gtactgctct cccacgcgcg cggcccgttc gagcagacgg ctgtgcaggt
      361 agaacacgtc gcccgggtag gcctcgcggc cggacggccg cttcagcacg agcgagacct
      421 gacggtaggc gacggcgtgc ttggacagat cgtcgtacac gatgagcgcg tccatgccgt
      481 ggtccatgaa ccactcgccc atcgccgcgc cggcaaacgg cgccaggtac tggttggtgg
      541 ccgagtcggc cgccggggac gcgatgatga tcgtgtacgg catcgcgccg gcttcctcga
      601 gcacggcgac gatgcgcgcg atcgtggact gcttctgccc gatagcgacg tagatgctgt
      661 agagcggacg atagtccttg ttcccggccg cctccgcttc gcggttcagc cggccctggc
      721 tgatgatcgt atcgatgcag atcgtcgtct tgccggtcga acggtcgccg atgatgagct
      781 cgcgctggcc gcggccgatc gggatcatcg cgtcgatggc catgatgccg gtctgcaccg
      841 gctggctgac tggtttgcgc cgcatgatgc cgggcgcgat cttctcgacc ggataggcct
      901 cgctgctggc gatcggccct ttgccgtcga ccggctcccc gagcatgttg accacgcggc
      961 cgagcagccc cttccccacc gggacctgca ggagcttccc cgtcgcggtg acttcgttgc
     1021 cctcctcgag gctcgtgtaa tcgccgagga tgatgacgcc gacttcggtt tcctcgaggc
     1081 tgagcgcaat gcccgtgatg ccgttgccga ggtcgagcat ctcgttgagc atcacgtcgc
     1141 ccagcccctc gacccgggcg acgccgtcgc cgatctcacg gatgatgccg atgctgcgct
     1201 tgagggccgt cgtctttgcg cccgcgatct gcgcttcgat gtcctgtatc agattcgcca
     1261 tggatctact gccccctgcc aacggccccc tgcttactga aatttcgcct cgagcgcggc
     1321 cagccggcct tggatgctgc cgtcgtagac gtcgctgccg acggtgaccc gcacgcctcc
     1381 cagcaaggcg gcgttgccgg agaacgaggt cgtaaggccg cggccgtgga tgcgcgtcag
     1441 gctcccttcg atgctcgcgc gcacgtcgtc gggcagcggc gtcgcgctct ccactttggc
     1501 gctgtgagcg gcgcgatcga gccggaccag gcgctgaaag cgcgtcaaca cggccagggc
     1561 ggccggcccg ccaccgccgg cgacacgttg cacgatgccc cggacgcggc cctcgtcgag
     1621 cgccccgttc accaggcacg ctcggtaaag ccgcttggcg tcacgctgaa tctgtcggcg
     1681 cgtcttcatt gcaactgtct tgcggtttcc tcggccagac gccgctcgtc gtcggaggtc
     1741 agtaccttgc ccaccacagc cgtcgtcgtc tggacgacca gccgtccgac ttcgcggcgg
     1801 agctccgcca gcatcctcgc gtgctcctgt tcggccgcct cgcgcgcctt caccaggatc
     1861 tgctcggccg cggcgaccgc gcgctgcgtc tcctgctcgc tcaacttctt ggcaaggtcg
     1921 cgggcctcgg cgacgagctt cgtcgactgg gcctgggcct cggcgatcac gccctggcgc
     1981 tgcgcctcga tcccggcaag cgcagcgttg atcttctcgg tgttctccag gccctgggcg
     2041 atctgcttgc gccgcgtctc gagcatctgc agcacaggct tgtacgccag ccaatagagc
     2101 agcgcacaga cgatgctgaa gctgatgatc tgggccgtca gatggggcca gttcacgcca
     2161 aaggtacgcg cgatctgctc gacctggccg ccgccctgct cctgcatcat caaggcgaga
     2221 atggtcatcg ctgtctcccc acgctcctcc ccgtcactgc gcctggacga ggaacagcgc
     2281 gtagaacacg atcgcttccg cgaaggcgat cgacaggatc gactggacca gaatcgccgt
     2341 ggccgcgccg gggttccggc ctacggcttc cgacgctttc ataccgatga ggcccacgcc
     2401 cagggctgcg cccagggccg cgaggccaac gtgcaggttt cccatcacca tagattttct
     2461 cgtccttcct caggtgaggc cgccgcgtcc tcaccgtgcg atcgagcgtc agccgctcgt
     2521 cgttcagcca tgcgcggcag aaccctcttc gtgatgctgg caaatcagca gcgtgaacac
     2581 agcagtcagc aacatgaaca cgagcgcctg gaccaggccg accagcagtt ccaggaaata
     2641 gaacgggatc tgcacgagcc agccgatgtt cggcaccgcc gccatggtct cgagcatgtt
     2701 ctcgccagca aagatgttgc cgtaaagacg aaagctcagc gacacgggcc ggaacaggat
     2761 cgagacgacc tccagcaagc ccgccgcgag aaacacgacg atcatgagta ccttgaggaa
     2821 gcccgtgttc gagcccttgg gcgcgaacaa ctccttcgcc atgcccagcg ggccgacctc
     2881 tcgaagcgcc cagacgaccc acatgacgaa gaagatcagc gccatcgcga gcgtcatgtt
     2941 cacgtcggcg ttggcgccgc ggaagaacgg ctcctccagc acgaacccgt gctcgccctg
     3001 atggccccat cccatcgagc ccacgcccgg aatcagcccg aaccagttcg acgcgaggat
     3061 gaagatgaag acggtcgcga agaaccagaa cgtgcgcttg accaggggcc cgatgatgcc
     3121 ttcgagaaag gtgtacagac cctcgacgag ccattccaga aagttctgcg atccggtggg
     3181 cacctgcttc atgttccggg tggcgattcg cgcgaaggca atcagcgcca ggccaacgat
     3241 ccaggtgacg accatcgaat tggtcaccgg gaacccgaac gggcgggcga tctccacggc
     3301 gctctgcggc agcccgtggt gaccttcctc gcctacggcg agtaatgggg cggcgttcaa
     3361 cccgatcgcc agcacggtca gcagaattcg cgccaaagtc gctcttcctc tacttatcac
     3421 gttctcgaac gtcttgcacc gtcttgacat ccctgcccgc tgcaggcggg cctcacccgc
     3481 caagggcgat ggtgatcgcc accagaatga ctcccaggcc aaccgtaccc agaccgacga
     3541 gccaggccgg cctcctggcg tattttccga gggaccaccc cgtcacaaag agcatcacaa
     3601 gcgcgactgc gttcgacgta cgcagcgcga gcgcgggctg cgggacaacc aaaaagggta
     3661 tcacgaccgg aaacgtcgac aggaagacga gcaggaagac gccgaacgca cccttcagca
     3721 ctcgtccttc cacggccacc cgcgccggtg gctcgggttg gtgacgcagc cgttcgcgca
     3781 gcgcgtcgat gtcgtcgggc gtcagcacct cggcgaccgg ggacggaagc gcgtcgagga
     3841 ggagacgctg tgcctcgtcg gtccgggcgc cccgaaccgc gcgcagaatc tcgagccccc
     3901 gcgcgcgctg cgtgaaattg gcgagcaggt agaagaccgc gtcgatgagg ccccacgcga
     3961 ggttgcaccc gatggcgccg aggaggatgg tccggttctc ggcctctccc ccttcgacga
     4021 cgctgatcga ggaggtgaac gtcagaacca tgatgatgcc gaacatgacc tcggtcgtgc
     4081 gctcgatcgg gtccaggatg cgttctgaag ctgccaacct ctacctcatg cgtggcatgt
     4141 gtcgacgtcc ccggacgcac caagtgcacg ccc
//
LOCUS       DBNGSH010000267         2310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3756859, whole genome shotgun sequence.
ACCESSION   DBNGSH010000267 DBNGSH010000000
VERSION     DBNGSH010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3756859"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="AC16CY_06215"
     CDS             <1..280
                     /locus_tag="AC16CY_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HIR3353412.1"
                     /translation="MPTIAAFMQLLPSGFTGQPYRQTDATVYVVVEGQGRTRIGGTTF
                     DWAPRDIFVAPSWTTVAHESRSDAVLFSLSDRPAQQALGLWREEAPLR"
     gene            327..1112
                     /locus_tag="AC16CY_06220"
     CDS             327..1112
                     /locus_tag="AC16CY_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3353413.1"
                     /translation="MGRSVMCIAVLVLAAPALAFAQAASDVAEGKVLFNGLCVTCHGF
                     EGAGGMGPPLNRPNLRSAPTDAELRTIISDGIPDRGMPRVRRVLEDELRQLVAYVRSL
                     GRVARPPVKGNPQQGAQLYAKLGCAACHIVGGQGGSLGPELSVIGWLRGADYLRQAIL
                     EPAARLPQGTLPIPSRGYSEYLPVTVVMRDGRELRGVRLNEDVFTIQFRDGSGRFHSV
                     RKSEAALVRKETATSLMPSYATRLAAAEADDLVAYLSTLGGAR"
     gene            1109..>2310
                     /locus_tag="AC16CY_06225"
     CDS             1109..>2310
                     /locus_tag="AC16CY_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3353414.1"
                     /translation="MSDRRRWVLALVTLVLAVAGRLAAQVPYERLLKAASEPQNWLTY
                     AGSYKSQRYSALDQINRKNVSSLKVAWAYQIQRAGIVETSPLVVDGVMYMTEPPSTVT
                     ALDVRSGRPLWSYSPTIPPDVIIIGSPPVNRGVAILDDHVFVGTVHGHLIALDARSGV
                     VRWDITVEDNRQGFYLTLSPLALDGKVIVGVSGAEAGIRGFVDAYDAKSGKRLWRTYT
                     IPAPGEPGSETWGKGDGWKTGGGSTWLTGSFDPELNLVYWPTGNPAPDWNAENRPGDN
                     LYTDSVLALDAGTGRMKWYFQFTPEDVHDWDANETLVLFEAAIGGRERRLLAQANRNG
                     FYYVLDRATGEFLTGVPYAKQTWAEGLDAKGRPIRKKGIAPSLEGTLVFPNIIGAANW
                     FSPSYSPQT"
BASE COUNT          378 a          758 c          790 g          384 t
ORIGIN      
        1 gatgcccacg atcgcggcct tcatgcagct gctgccctcg gggttcaccg ggcagccgta
       61 ccggcagacg gacgcgacgg tgtacgtggt ggtcgaaggt cagggccgca cgcgcattgg
      121 cggcaccacc ttcgactggg cgcctcgcga catcttcgtc gcgccctcgt ggaccaccgt
      181 tgcgcacgag tcccggtccg acgccgtcct cttcagcctg tcggaccggc ctgcgcagca
      241 ggcgctgggg ctgtggcggg aagaggcgcc tctcagataa cggggttggt atactgcgcc
      301 tccgatatat cagttcgcga ggtctcatgg gcaggtccgt catgtgtatc gcggtgctgg
      361 tgctcgccgc gccggcgctc gccttcgctc aagctgcgtc ggacgtggcg gaaggcaagg
      421 tcctcttcaa cggactgtgc gtcacctgcc acgggttcga gggagcgggc ggcatgggtc
      481 ccccgctcaa tcgtccgaac ctgcggtcgg cgccgaccga cgcggagctc cgaaccatca
      541 tcagcgacgg catccccgat cgcgggatgc cccgagtgcg gcgcgtgctc gaagacgagc
      601 tgcgtcagct cgtggcctac gtgcgatcgc tcgggcgcgt cgctcgtcca ccggtcaaag
      661 gcaacccgca gcagggcgcg cagctctacg ccaagctggg gtgcgcggcc tgccacatcg
      721 tcggcggcca gggcggaagc ctcgggcccg agttgagcgt gatcggatgg cttcgagggg
      781 ctgactacct ccgtcaggcc atcctcgagc ctgcggccag gctgccgcaa gggacgctgc
      841 ccattccttc gcgcggctac agcgagtatc tgcccgtcac cgtcgtgatg cgcgacgggc
      901 gcgaactgcg cggcgtgcgg ttgaacgagg acgtcttcac gattcagttt cgggacggga
      961 gcggccggtt ccactccgtc cgcaagagcg aggccgcgct cgtcaggaag gaaaccgcga
     1021 cgagcctgat gccgagctac gccactcgtc ttgcggccgc agaagccgac gatctcgtgg
     1081 cgtacctgtc cacactcgga ggtgcccggt gagcgatcga cggcgatggg tgctggcgtt
     1141 ggtcacgctc gtcctcgcgg tggcgggacg gctcgctgcc caggttccgt acgaacgcct
     1201 gctgaaagcg gcgagcgagc cgcagaactg gctgacctac gccggttcct acaagtcgca
     1261 gaggtattcg gcactggacc agatcaaccg gaagaacgtg tcttctctga aggtggcctg
     1321 ggcgtatcag attcagcgag ccggcattgt cgagacgtcg cccctggtgg tggacggggt
     1381 gatgtacatg acagagcctc cgagcacggt gacggcgctc gacgtgcgca gcggccggcc
     1441 gctctggagc tactcgccca cgatcccgcc cgacgtcatc atcatcggtt caccgccggt
     1501 caatcgcggc gtggcgatcc tcgacgacca cgtctttgtc ggcaccgtgc acgggcacct
     1561 gatcgcgctc gacgccaggt ccggcgtggt tcgatgggac atcaccgtcg aggacaacag
     1621 acaggggttc tacctcacgc tctctccgct ggcactcgac ggcaaggtca tcgtcggcgt
     1681 gtcgggcgcc gaagccggta ttcgcggttt cgtcgacgct tacgatgcga agtcgggcaa
     1741 gcggctgtgg cgcacctata cgatccccgc gccaggggag cctggttccg agacgtgggg
     1801 gaaaggcgat ggctggaaga ccggtggcgg ctcgacatgg ctgaccggtt cgttcgatcc
     1861 cgagttgaat ctcgtgtact ggccgacggg caatccggcg cctgactgga acgcggagaa
     1921 ccggcctggc gacaacctct acaccgactc cgtgctcgcg ctcgacgccg gcaccggccg
     1981 gatgaagtgg tacttccagt tcacgcccga ggacgtgcac gactgggacg ccaacgagac
     2041 gctcgtgctc ttcgaggccg cgatcggagg acgcgagaga aggcttctcg cccaggccaa
     2101 tcgcaacggc ttctactacg tgctcgatcg cgcgacgggg gagttcctga ccggcgtgcc
     2161 ctacgcgaag cagacctggg ccgaggggct cgacgccaag gggcgtccga ttcgaaagaa
     2221 agggatcgcg ccgagcctcg aggggacgct cgtcttcccg aacatcatcg gcgccgccaa
     2281 ctggttcagc ccgagctaca gcccgcagac
//
LOCUS       DBNGSH010000268         8642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2908059, whole genome shotgun sequence.
ACCESSION   DBNGSH010000268 DBNGSH010000000
VERSION     DBNGSH010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2908059"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="AC16CY_06230"
     CDS             complement(<1..706)
                     /locus_tag="AC16CY_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR3353415.1"
                     /translation="MSKVIGIDLGTTNSVVAVMEGGEPVVIVNQEGSRTTPSIVAFNK
                     DGERLVGQVAKRQAVTNADSTVFSIKRFMGRKHAEVPQEIGRVPYAVVKAGNGDAWVD
                     VRGKKYSPPEISAMVLQKLKTAAEDYLGQKVTDAVITVPAYFNDAQRQATKDAGKIAG
                     LNVLRIVNEPTAAALAYGLDKKKDETIAVYDFGGGTFDVSILEVGEGVVEVKSTNGDT
                     HLGGDDLDQRIVDWMAA"
     gene            890..1369
                     /locus_tag="AC16CY_06235"
     CDS             890..1369
                     /locus_tag="AC16CY_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3353416.1"
                     /translation="MRSAVLEFSFLPITESGELVEDLRRVFAELSSTLPPEQRVSSGE
                     YHPSLDILETADTVEIVVDLAGVPAASARVLFRGGVLIVAGEKAPAPVPADSDFHFLE
                     RGFGRFARAVQLTGAFEIGQARAVLRAGELTIVLPKRPDRRGRAQTIPITTSSTPPA"
     gene            1366..2196
                     /locus_tag="AC16CY_06240"
     CDS             1366..2196
                     /locus_tag="AC16CY_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIR3353417.1"
                     /translation="MNILFIGDIVGRPGRELLRKGLRGLVDHHDVDFVIANVENAAAG
                     FGITKDIGDAILDWGVDVMTSGNHIWDKREALDYIAAEPRLLRPANYPAGVPGRGSCV
                     TASRDGRAVGVVNVMGRVFMLNIDDPFSVVLREIESLKHKTKVVIVDFHAEATSEKIA
                     MGWHLDGKATLVVGTHTHVQTADERVLPNGTAYLTDAGMTGPHDSIIGMEREPSLARF
                     LTGLPSKLEPATGNPRLNGVLVSADDRTGRASSVTRVSYSEVEMAAFASRAESRVPSR
                     "
     gene            2193..3581
                     /gene="xseA"
                     /locus_tag="AC16CY_06245"
     CDS             2193..3581
                     /gene="xseA"
                     /locus_tag="AC16CY_06245"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR3353418.1"
                     /translation="MSLFDLPFEEPEEDAPVPPSPRRILTVSELTGRIRTLLEERFFE
                     IWVEGELSNCRVWNTGHMYFTLKDAGAQIKGVMFRSSLRLLRFKPQDGHRVVARGRVS
                     VYDPKGEYQIVCEHLEPEGLGALQLAFDQLKQRLDAEGLFDARRKRPLPALPRKIGIV
                     TSLDGAAIRDIINVLQRRYANAHLVIRPTRVQGEGAALDIARGLRAIAKVKGVDVIVV
                     GRGGGSMEDLWAFNEEVVARAIAGAPVPVISAVGHETDVTIADFVADLRAPTPSAAAE
                     LVVARKDEFTSRIDRLTRRVSSALSSRLHRLESRVRVLEGRPGFAGVQGRVALRARHA
                     DELGFDLRRAVEDRLASRRRRYQALRTTLERNDLSRRFADVRHTLVAGDARLGLAMQQ
                     RHHAFSGRLSTVAARLDGLSPLAVLGRGYAVCWAADRTSIVRDASSVGAGDRVAVTLA
                     RGELECEVIRTR"
     gene            3659..3916
                     /locus_tag="AC16CY_06250"
     CDS             3659..3916
                     /locus_tag="AC16CY_06250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR3353419.1"
                     /translation="MDPTIKDFETAIAELETIVKKLEEGDLTLEASLQLYERGVHLSR
                     FCHARLEEAERRIEILNERGELKPAPSALGAQHAAPAKVED"
     gene            3913..4833
                     /locus_tag="AC16CY_06255"
     CDS             3913..4833
                     /locus_tag="AC16CY_06255"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3353420.1"
                     /translation="MTGIAAYLDTSRVLVEAALGRVLPGPPGCPDVVAKAMQYSLMAG
                     GKRLRPILCLASAEAVGGDRLMALPAACAIELIHTYSLIHDDLPAMDDDTMRRGRPTL
                     HVVAGEGMAILAGDGLLTEAFHLLAREPASRDPQIVARKIRVIDTIAAAAGPEGMVGG
                     QAIDLASVTVDSATGQAPLLDAKALRWMHGMKTGALIRASAASGAIMGGGSDDEIAAV
                     DGAAAEFGLAFQIVDDILDVEGASAHLAKTAGKDAAAGKPTYPGLYGLEASRRMAAEC
                     LDRAAATLRSAGIADDRLLAIGTWIVERSK"
     gene            4906..5637
                     /locus_tag="AC16CY_06260"
     CDS             4906..5637
                     /locus_tag="AC16CY_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR3353421.1"
                     /translation="MSKLRLDQVVVERGLAASRERARALVLAGQVKVDGQVITKAGAA
                     IGAEALVELIAPDHPYASRGGLKLAHALDVFQIRVDGRQCLDIGASTGGFTDVLLQRG
                     AARVVALDVGHGQLDWKLRNDPRVEVVERFNARHLTPSDTHGKIDLATIDVSFISLRQ
                     ILPVVPPLLGPDADVVALVKPQFEAGRAEVRKGIIVDPAVHARVLEEVAAAGAEVGLT
                     RVAATPSPITGQKGNVEFLVHFRQS"
     gene            5634..6527
                     /locus_tag="AC16CY_06265"
     CDS             5634..6527
                     /locus_tag="AC16CY_06265"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR3353422.1"
                     /translation="MSDQISRVGLVAKQGLRGAANHIDRLAAWLKERGIEPVFDTDTA
                     SLTGWGGETRSRDDLPGDVDLVVVMGGDGTLLSMARRTAQAGRDAPILGVNFGSLGFL
                     TETRIDELYTVLESVLDGTATYDDRAMLAADAYRAREHFDSRIVLNDVVFTKAALSRI
                     IELSISVGAGLVTRVRADGLIVATATGSTAYNLAAGGPIIHPRTDAIVLTPIAPHTLT
                     NRPIVIPASEVVEVRPEVVAGLHDIHVTYDGQIGYPLQQGDVVRIRTSERTLRLVRAP
                     TRSYFEVLREKLMWGERAGRE"
     gene            6536..7393
                     /locus_tag="AC16CY_06270"
     CDS             6536..7393
                     /locus_tag="AC16CY_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR3353423.1"
                     /translation="MQIHRALTMLVTTLAAMTAVVTIAQPKRAPVTSPRIYVFDAGNI
                     KGLDPKLFNFTREELKEVDFVNIAYLIAHPKGTLMFDAGAVTDSHFKGDGTPVTEGVV
                     SASRPLKPQLAAAGYAPAEVTYLAMSHYHSDHTANANDFAGATWIVQKAERDAMFADK
                     PQGIIQPAHYSALRNAKTRVLDNQDLDVFGDGTVVVMSTPGHTPGHQVLFVRLAKRGP
                     VLLAGDLYHYPEERTTGRIPTFEFNAEQSRASRQKVEAFVKQMKAELWIEHDIATHAK
                     LPRAPGFVE"
     gene            7587..7823
                     /locus_tag="AC16CY_06275"
     CDS             7587..7823
                     /locus_tag="AC16CY_06275"
                     /inference="COORDINATES: protein motif:HMM:NF017451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3353424.1"
                     /translation="MGKINSFRELLVWQKSMDLAERSYRLTQRFKREDQPVLGHEIRK
                     SSVSMPSNIAEGFGRHHTAEYIHHLIRALDRGRG"
     gene            complement(7929..>8642)
                     /gene="mltG"
                     /locus_tag="AC16CY_06280"
     CDS             complement(7929..>8642)
                     /gene="mltG"
                     /locus_tag="AC16CY_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR3353425.1"
                     /translation="GEVVTHPITFPEGLTIREMAAIYESRGFGASRDFIRAAGDASLI
                     RDLDPEAGDLEGYLFPETYALPRGTPATRLVEMMVERFRAVYTSLPAGEGDELELSLR
                     QLVTLASLVEKETGKGDERALVSAVYRNRLKRRMPMQADPTVVYALVKAGTYDGNIQK
                     HDLSFDSPYNTYKYAGLPPGPIASPGRAALEAARAPADVTYVYFVSRNDGSHVFADTL
                     SEHNANVHEHQVLYFRKRR"
BASE COUNT         1434 a         2913 c         2859 g         1436 t
ORIGIN      
        1 cggccgccat ccaatccacg atgcgctgat cgaggtcgtc tccgccgagg tgcgtgtctc
       61 cattggtgga cttgacctcg accacgcctt cgccgacctc gagaatcgac acgtcgaagg
      121 tgccgccacc gaagtcatac acggcgatcg tctcgtcctt cttcttgtcg agcccgtagg
      181 ccagggccgc cgcggtcggc tcgttcacga tgcggagcac attgaggccg gcgatcttgc
      241 ccgcatcctt cgtggcctgc cgctgcgcgt cgttgaaata ggccggcacg gtgatcaccg
      301 cgtcggtgac tttctggcca aggtagtcct cggccgcggt cttcagcttc tggagcacca
      361 tcgcggagat ctcgggcggc gagtacttct tgccgcgcac gtccacccag gcgtcgccgt
      421 tgccggcctt gacgaccgcg tagggaacgc ggccgatttc ctgcgggacc tcggcgtgct
      481 tgcgccccat gaaccgcttg atcgaaaaga ccgtgctgtc ggcgttggtc accgcctggc
      541 gcttggcgac ctgcccgacc agacgctcgc cgtccttgtt gaacgccacg atggacggag
      601 tcgtccgcga accctcctgg ttcacgatga cgactggttc accaccctcc atcacggcga
      661 cgaccgagtt ggtcgtcccc aaatcgattc caatgacttt gctcatatgg gttcctctgc
      721 ctcgcacgag cgatagaata gcacgatatg agtttatgtc aagcagttaa tcttagtctt
      781 ctcgcatcat atattggcca gctcgacatt gaccacgtaa cccctagaaa accttgacca
      841 ttccaggcct cgaccacttc cccactccca ggcccgcgtc cgtgcgatca tgaggtccgc
      901 cgtgctcgag ttctcgtttc tgcctatcac cgagtcggga gaactggtcg aggacctccg
      961 acgggtcttc gcggagctct cctccacgct gccgccggaa cagcgcgtct cctctggcga
     1021 ataccacccg tcgctcgaca tcctcgagac agcggacacg gttgaaatcg tcgtcgacct
     1081 cgcgggcgtt ccagcggcgt ctgcgcgcgt gctgttccgc ggcggcgtcc tgatcgtggc
     1141 tggcgagaaa gcgccggccc cggtgccggc ggactccgac tttcacttcc tcgagcgtgg
     1201 cttcggccgc tttgcgcggg ccgtgcaatt gaccggcgcg ttcgagatcg ggcaggcgcg
     1261 ggcggtcctc cgcgcgggcg aactgaccat cgtccttccc aaacgtcccg accggcgcgg
     1321 ccgcgcgcag acgatcccga tcaccaccag cagtacgccg ccggcatgaa catcctcttc
     1381 atcggcgaca tcgtcggacg gcccggccgc gagctcctcc gaaaagggct gcgcgggctc
     1441 gtcgatcatc acgacgtcga tttcgtgatc gccaacgtgg agaacgcggc cgccggattc
     1501 gggatcacca aggacatcgg cgacgcgatc ctggactggg gcgtggacgt gatgacgtcg
     1561 ggcaatcaca tctgggacaa gcgcgaggcg ctcgactaca tcgcggcgga gccgcgcctg
     1621 ctgcgtccgg ccaactaccc tgccggcgtg cccggacgcg gctcgtgcgt caccgcctcc
     1681 cgtgacgggc gcgccgtcgg cgtcgtcaac gtgatggggc gcgtgttcat gctgaacatc
     1741 gacgacccct tctccgtggt gctcagggag atcgagtcac tgaagcacaa gacgaaagtc
     1801 gtcatcgtgg actttcatgc cgaggccacg agcgagaaga tcgcgatggg gtggcatctc
     1861 gacggcaagg ccacgctggt cgtcggcact cacacacacg tgcagaccgc ggacgagcgc
     1921 gttctcccga acgggacggc gtatctgacg gacgcgggaa tgacgggtcc gcacgactcg
     1981 atcatcggca tggagcgcga gccgtcgctc gcacggttcc tcaccggtct tccctcgaag
     2041 ctcgagccgg ccacgggcaa ccccaggctc aacggcgtgc tcgtgtctgc cgacgatcga
     2101 accggccgcg cttcgtccgt cacgcgggtg agctacagcg aggtcgagat ggccgccttt
     2161 gcctcgcgcg ccgaatcccg ggtgccgagc cgatgagcct tttcgatctg cctttcgagg
     2221 aacccgaaga agacgccccc gtgccgccgt cgccgcggcg gatcctcacc gtgtcggagc
     2281 tcacgggccg catcagaacg ctgctcgagg agcgattctt cgagatctgg gtggagggcg
     2341 agctgtcgaa ctgccgcgtg tggaacacgg gccacatgta cttcacgctc aaggatgccg
     2401 gcgcccagat caagggcgtg atgttccggt cgtcccttcg cctccttcgg ttcaagcctc
     2461 aggacggcca ccgtgtcgtg gcgcggggac gcgtcagcgt gtacgacccg aaaggcgagt
     2521 accagatcgt ctgtgaacac ctcgagcccg aaggccttgg cgctctccag ttggcgttcg
     2581 accagttgaa gcagcgattg gacgcggaag ggctgttcga cgcgcggcgc aagcgcccgc
     2641 tgccggcgct gccgcgcaag atcgggatcg tcacgtcgct cgacggggcc gccatccgcg
     2701 acatcatcaa cgtgctgcag cggcgctacg ccaacgccca cctcgtcatc cgccccaccc
     2761 gcgtgcaggg cgagggcgcc gcactcgaca tcgcgcgcgg cctgcgggcc atcgcgaagg
     2821 tcaagggcgt ggacgtcatc gtggtcgggc gcggcggtgg ctcgatggag gacctctggg
     2881 ccttcaacga agaggtcgtc gcgcgcgcga tcgcgggggc accggttccg gtgatctccg
     2941 ccgtcggaca cgagaccgac gtcacgatcg ccgacttcgt cgccgacctc cgcgcgccga
     3001 cgccgtcggc ggcggccgaa ctggtcgtgg cgcgcaagga cgagttcacg tcgcgcatcg
     3061 accggctcac ccggcgcgtg tcgtctgccc tgtcgtcgcg gctccatcgc ctggagtcgc
     3121 gcgtccgcgt actcgagggg cgacctggct tcgccggcgt gcagggccgt gtcgcactgc
     3181 gggcccgtca cgccgacgag ctcggcttcg accttcgacg tgcggtcgag gaccggctgg
     3241 cgtcgcggcg gcggcggtac caggcgctgc gaacgacgct cgagcgaaac gatctttctc
     3301 gacgctttgc cgatgtcaga cacacgctcg tcgcgggaga cgcccgcctc ggcctggcga
     3361 tgcagcagcg gcatcacgca ttcagcggcc ggctgagcac ggtcgcggcc cggctcgacg
     3421 gactcagccc gctggctgtt ctgggccgcg ggtatgccgt atgctgggct gccgaccgga
     3481 cttcgatcgt gcgcgacgcg tccagcgtcg gcgctggcga tcgcgtcgcg gtcacgctcg
     3541 cgcgcggcga gctcgaatgt gaagtgattc gcactcggta ggcagtgggc gacgtagagc
     3601 gcgggcttca gcccgcgcgt gtagcggggc ttcagcccgc gcgtggtaag cgacaggcat
     3661 ggatccgacc atcaaggact tcgagaccgc catagcggag ctcgaaacca tcgtcaagaa
     3721 actcgaggaa ggcgacctga cgctcgaagc gtcgctgcag ctgtacgagc gcggcgtgca
     3781 cctgtcgcgg ttctgccacg cgcgtctcga ggaagccgag cgtcgaatcg agatcctcaa
     3841 cgagcgcggc gagctcaagc cggctccctc cgcactcggc gcgcagcacg cagccccggc
     3901 gaaggtcgaa gattgaccgg cattgccgcg tatctcgaca ccagccgggt gctggtggag
     3961 gccgcgctcg gccgggtgct gcccgggcct cccggctgtc ccgacgtcgt cgcgaaggcg
     4021 atgcagtaca gcctgatggc cgggggcaag cggctgcggc cgattctctg tctcgccagc
     4081 gccgaagccg tgggcggcga tcggctgatg gcgctgcccg ccgcctgcgc gatcgagctg
     4141 atccacacct actcgctcat tcacgacgac ctgcccgcca tggacgacga cacgatgcgg
     4201 cgcggcaggc ccacgcttca cgtcgtggcc ggcgagggca tggcgatcct tgcgggcgac
     4261 ggcctcctga cggaggcgtt tcatctgctg gcacgcgagc ccgcaagccg ggacccgcag
     4321 atcgtcgccc gcaagattcg cgtcatcgac acgatcgccg cggcggcggg accggaaggc
     4381 atggtcggcg gacaggccat cgatctcgca tcggtcaccg tggattccgc gacgggtcag
     4441 gcgccgctcc ttgacgccaa ggcgctgcga tggatgcacg ggatgaagac cggggccctc
     4501 atccgtgcgt ccgcggcgtc gggcgcaatc atgggcggcg gaagcgatga cgagatcgcg
     4561 gcggtcgatg gcgcggccgc cgaattcggg ctggcgtttc aaatcgtcga tgacatcctc
     4621 gacgtggagg gcgcgtcggc ccatctcgcc aagaccgccg gaaaggatgc cgccgcggga
     4681 aagccgacct atccgggact ctacggtctc gaggcgtcgc gccggatggc ggccgagtgc
     4741 ctcgaccgcg cggcggccac gcttcgatcg gccggcatcg ccgacgatcg tctgctcgcg
     4801 atcgggacgt ggatcgtcga acggtcgaag tgaactcgaa ggggaaaggg ggcagggggc
     4861 agagggcagg gggaaggttg ggagttggaa gttgggagtt gggagttgag taagctccgc
     4921 ctggaccagg tcgtcgtcga acgaggcctc gccgcgtcgc gcgagcgggc gcgcgcgctc
     4981 gtcctggcgg gtcaggtcaa agttgacggg caggtgatca ccaaggccgg cgcggcaatc
     5041 ggcgcggagg ccctggtaga gctgatcgcc cctgaccacc cgtacgccag ccgcggagga
     5101 ttgaagctgg cgcacgcgct cgacgtcttc cagatccgcg tggacggccg gcagtgtctc
     5161 gacatcggcg cgtccaccgg gggcttcacc gacgtcctcc tgcagcgcgg ggcggcccgt
     5221 gtcgtcgcac tcgacgtcgg ccatggacag ttggactgga agctgcgcaa cgacccgcga
     5281 gtcgaagtcg tcgagcgctt caacgcccgc catctgacgc cgtccgacac gcacgggaag
     5341 atcgacctcg ccacgatcga cgtgtcgttc atctcgctcc ggcagattct tcccgtcgtg
     5401 ccgccgctgc tcggccctga cgccgacgtc gtcgcgctcg tcaagccgca gttcgaggcc
     5461 gggcgcgccg aggtgcggaa ggggatcatc gtcgatccgg cggttcacgc tcgcgtgctc
     5521 gaggaagtgg ccgccgcagg cgctgaggta ggattgacgc gcgtggcggc gacgccttca
     5581 cccatcaccg gccagaaggg caacgtcgag ttcctcgtcc actttcggca atcatgagcg
     5641 accagatctc gcgcgtcggc ctcgtggcca agcagggcct gcgcggcgcg gcgaatcaca
     5701 tcgaccggct cgccgcgtgg ctgaaggagc ggggcatcga gcccgtgttc gacaccgaca
     5761 cggcgtcgct gaccggctgg ggcggcgaaa cgcgctcgcg cgacgacctc ccaggagacg
     5821 tggacctcgt cgtcgtcatg ggcggcgacg gcacgctgct cagcatggcg aggcgcaccg
     5881 cgcaggccgg gcgcgacgcg cccattctcg gggtgaactt cggcagcctc gggtttctca
     5941 ccgagacgcg gatcgacgag ctctacaccg tgctcgaatc ggtactcgat ggcacggcaa
     6001 cctacgacga tcgcgcgatg ctggcggcag acgcctaccg agccagggag cacttcgact
     6061 ctcgcatcgt cctgaacgac gtcgtcttca cgaaagcggc gttgtctcga atcatcgagc
     6121 tgtcgatttc cgtcggcgca ggcctcgtca cgcgcgtcag ggccgacggc ctgatcgtgg
     6181 cgacggcgac cggctcgacc gcgtacaacc tggcggcagg cggcccgatc attcacccca
     6241 ggacggacgc catcgtgctg acgccaatcg ctccgcacac gctcacgaac cggccgatcg
     6301 tgatcccggc cagcgaggtc gtcgaagtgc ggcccgaggt cgtcgccggc cttcacgaca
     6361 tccacgtcac ctacgacggc cagatcggct atcccctcca acagggcgac gtcgtgcgca
     6421 tccgtacgtc ggagcggacg cttcggctgg tcagggcccc gacgcgcagc tacttcgagg
     6481 tccttcgcga aaagctcatg tggggcgaac gcgcaggaag ggagtgagac aaccgatgca
     6541 gatccacaga gcccttacga tgctcgtgac gacgcttgcg gctatgacgg ccgtcgtcac
     6601 gatcgcccag cccaagcgcg cgcccgtcac atctccacgg atctatgtct tcgacgcggg
     6661 caacatcaag gggttggatc cgaaactgtt caacttcacc cgcgaggaac tgaaggaagt
     6721 ggacttcgtg aacatcgctt atctcatcgc gcaccccaag ggcacgctga tgttcgacgc
     6781 cggcgcggtg accgacagcc atttcaaggg ggacggcacg ccggtgaccg agggtgtcgt
     6841 gtccgccagc cgcccgctca agcctcaact ggccgccgcc ggatacgcgc cggccgaggt
     6901 gacgtacctg gcgatgtcgc actaccactc cgatcacacc gccaatgcga acgacttcgc
     6961 cggcgcgacc tggatcgtgc agaaggccga acgcgatgcg atgttcgcgg acaagccgca
     7021 gggcatcatt caaccggcgc actacagcgc gctcaggaac gccaagacca gggtgctcga
     7081 caaccaggat ctcgatgtct tcggcgacgg caccgtcgtc gtcatgtcga cgcccggcca
     7141 cactccgggc caccaggtgc tcttcgtgag actggccaag agagggcccg tccttctcgc
     7201 cggagatctg tatcactatc ccgaggagcg aacgaccggt cgcatcccga cgttcgagtt
     7261 caacgcggag cagtcgcgcg cttcgcgtca gaaggtcgag gcgttcgtca agcagatgaa
     7321 agcggagctc tggatcgagc acgacatcgc cacccacgcg aagctgccac gggcgccggg
     7381 gtttgtggag taactctgaa gggggcgggg ggcagggggc gggggacagg gctgtctcag
     7441 gatggaggcg acaggtctta ggcagcaggt atgcggttct tgccacgttc gggcgtcgat
     7501 cgcgttgcgg gaagtgcaca cttcaaggcg agattccgca aacccgtcga attatcgtgc
     7561 ggcaagtggc gtgctctctc gagagcatgg gcaagatcaa ttcctttcga gaacttctcg
     7621 tttggcagaa gtcgatggac ctggcggaac ggtcttatcg gttgacgcag cgcttcaagc
     7681 gtgaagacca acccgttctc ggtcacgaga tccgcaagtc ttcggtctcg atgccgtcga
     7741 acatcgcaga aggcttcggt cgtcaccaca cggccgaata cattcatcac ctcatcaggg
     7801 cattggaccg cggccgcgga tgatcgtcca cagtcctgtg gccctgatac ctccttcctg
     7861 atccctcaga cagccctgcc ccctgccccc ggccccctgc cccctacccc ctgcccccta
     7921 ccccctgcct acctccgctt cctgaagtag agcacctggt gttcgtgcac gttcgcgttg
     7981 tgttccgaca gcgtatcggc gaagacgtgc gaaccgtcgt tgcggctgac gaagtacacg
     8041 taggtcacat cggccggcgc ccgcgcggcc tcgagcgccg cgcggcccgg tgacgcgatc
     8101 gggccgggcg gcaggccggc atacttgtac gtgttgtagg gcgagtcgaa gctcagatcg
     8161 tgtttctgga tgttgccgtc gtaggtcccc gccttgacga gcgcgtacac gaccgtggga
     8221 tcggcctgca tgggcatcct gcgtttcaag cggttccggt acaccgccga gacgagcgcc
     8281 cgctcgtcgc cctttccggt ctccttctcg acgagcgacg ccagcgtgac cagctgcctg
     8341 agcgagagct cgagctcgtc gccttcccct gccggcagcg aggtgtagac cgcgcgaaaa
     8401 cgctcgacca tcatctcgac cagccgtgtc gccggggtcc cacgggggag cgcgtacgtt
     8461 tcgggaaaga ggtagccttc gaggtcgccc gcctccggat cgaggtctcg aatgagcgac
     8521 gcgtcgccgg cggcccggat gaagtcgcgc gacgccccga agccgcggga ctcgtagatc
     8581 gccgccatct cgcgaatggt cagcccctcc gggaacgtga tcgggtgcgt gaccacctcg
     8641 cc
//
LOCUS       DBNGSH010000269         9599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2059105, whole genome shotgun sequence.
ACCESSION   DBNGSH010000269 DBNGSH010000000
VERSION     DBNGSH010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2059105"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="AC16CY_06285"
     CDS             <1..318
                     /locus_tag="AC16CY_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3353426.1"
                     /translation="HNRAIAFISRRHLPLRETDDDFDVEDGRPNPEEALATGQQGRRL
                     LDAIQRLPVSLRQVVTLALEGLSYAEIGDVLGISETNVGARLTRARQRLRQLLLAHKG
                     GGE"
     gene            328..903
                     /locus_tag="AC16CY_06290"
     CDS             328..903
                     /locus_tag="AC16CY_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353427.1"
                     /translation="MQESDLELEGWQRQWQSQPASIDLRQKVEAGNRAMRLGLMSEIA
                     VTILMGGGSLLWAIVSERAEVLVLAVAVWGFITIAWTASILLRRGLWQPATSTTAAFL
                     NVSILRCERSLEAIVVQAVLYVVILAFDLAWLYWYRQETDVWTFLTRPVVLIVVGIVT
                     PILGAVALWYRGRVRRELENLRRLATSLPLP"
     gene            complement(881..1795)
                     /locus_tag="AC16CY_06295"
     CDS             complement(881..1795)
                     /locus_tag="AC16CY_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3353428.1"
                     /translation="MSIRPVKRVSLSKPTLEGAGVKLRRAFGFGDTTEYDPFLLFDDF
                     RNDNPHEYLAGFPWHPHRGIETITYVLAGTVDHGDSLGNSGSLGSGDIQWMTAGSGIL
                     HQEMPKGDAQGRMHGFQLWANLPASLKMTKPRYQDVQGKDVPEVVDDDGTRVRIVCGT
                     FWGRSGPVDGVAADPRYIDVSVPAGVRRSLPVEASRHAFAYVFAGSGSFRDASAPRPV
                     RTDIIGGDGEAVPDSIGNRSLVLFDSGDEITVQAGDEGIRFLLVSGQPIEEPVAWYGP
                     IVMNTRQELQQAFDELNRGTFIREGAAK"
     gene            1861..2079
                     /locus_tag="AC16CY_06300"
     CDS             1861..2079
                     /locus_tag="AC16CY_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353429.1"
                     /translation="MPESSDRSARIVMRRFESAADADRHDLEFWMQIPERERVLQVWR
                     LSQEIWRLRGERGDEPGLCRSVARVHRR"
     gene            2033..2464
                     /locus_tag="AC16CY_06305"
     CDS             2033..2464
                     /locus_tag="AC16CY_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353430.1"
                     /translation="MNPDFVDLLRAFIAADVRFLIVGAYALALHGRPRATGDLDVWID
                     ATPDNAARVMRALTSFGAPLADVSVHDFSRPGVTYQIGVAPGRIDLLTELTGLSFADA
                     WPGRLRRPFGEVDVDFIGREAFIRNKRATGRAKDLGDIEGM"
     gene            complement(3116..4699)
                     /gene="thiC"
                     /locus_tag="AC16CY_06310"
     CDS             complement(3116..4699)
                     /gene="thiC"
                     /locus_tag="AC16CY_06310"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3353431.1"
                     /translation="MIDFSSAYPNSRKVFEERTAALAPDGDPVTLRVPMREVTLGGGE
                     HPVRLYDTSGPQGHDVREGLPRLREPWIAARRARGHVGTQLYYARQGEITAEMEFVSA
                     REGLPAEFVRSEVARGRAIIPANVNHLELEPTIIGRNFLVKINANIGNSAVRSSIEDE
                     VEKLQWATLWGADTVMDLSTGKNIHATREWIIRNSAVPIGTVPIYQALEKVGGRPEDL
                     TWEVYRDTLIEQCEQGVDYFTVHAGVLLRFIPMTARRVTGIVSRGGSILAKWCLAHHQ
                     ENFLYTRFTEICEIMRAYDVSFSLGDGLRPGSIADANDEAQFAELKTQGELTRIAWEH
                     DVQVMNEGPGHIPMHLIKENVEKQLEWCGEAPFYTLGPLTTDVAPGYDHITSAIGAAM
                     IGWYGTAMLCYVTPKEHLGLPDRDDVKAGVIAYKIAAHAADLAKGHPRAQVWDDALSK
                     ARFEFRWEDQFNLAMDPVTARSYHDESLPAEGAKVAHFCSMCGPKFCSMELTQQIRDA
                     GMQEKSVEFRKSGEIYVKQ"
     regulatory      complement(4743..4839)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4876..5694
                     /locus_tag="AC16CY_06315"
     CDS             4876..5694
                     /locus_tag="AC16CY_06315"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3353432.1"
                     /translation="MRIAVLLSSIVAAAIEAGAQTTPAKPPAQPQPGTAQTKPAPAPQ
                     TRKPAPPASTRSGMAITVADARGVVLSGVRVEAIGPTMRDAETNASGQLNYPGLLPGV
                     YRLKFSGEGWIMFEREVTLRAGQVADVDVTLNAAPKPPPVPEPAPQPVVEAPAHGPKG
                     ETQTIGIEGLLEKEFIGKDPRRESLLSCSGNERATMIQLNGPMPQRLYENGDAVYYVL
                     GGDGTATIDGRKSPVRTNDFVSVPRGSAHAFDRTGKRALVLLAVLSGEPCERAK"
     gene            complement(5728..6165)
                     /locus_tag="AC16CY_06320"
     CDS             complement(5728..6165)
                     /locus_tag="AC16CY_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353433.1"
                     /translation="MVPRRDLVGGGVIAGLAALVAPGAAGAADGDAQSAAAINQLRET
                     FERQFDDVYTSKWRGVSRVRQQQRTWLAATHKYPDFIEIGLDVWDNVYDWHVVFQQPL
                     NVTRLGDGRYAMAFMFTTLLLRPEQAPNFVGYPFDGAGAARTR"
     gene            complement(6178..6588)
                     /locus_tag="AC16CY_06325"
     CDS             complement(6178..6588)
                     /locus_tag="AC16CY_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353434.1"
                     /translation="MRAIGLLVCLLSVTTCDENSPVGPTVQLNERFTLAPGQTAFVDE
                     AGLRVQFVHVTGDSRCPADAVCIQGGDAIVRVRVVDDASASAFHELHTGDSSRASVIA
                     AGARITLIDLQPFPFSSRTTAPGDYRATLMVTTP"
     gene            complement(6628..8610)
                     /locus_tag="AC16CY_06330"
     CDS             complement(6628..8610)
                     /locus_tag="AC16CY_06330"
                     /inference="COORDINATES: protein motif:HMM:NF023802.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR3353435.1"
                     /translation="MRASGLVVFVLLAGHADAQPAPGPLPILDVPFVSQSEALCGGAA
                     AAMILRYWGERGLDAESFAHLVDRSAGGIRTDRLVGDLRARGWSVAGLEGTGQRMSAE
                     LDRGRPVLALIQDRPGTFHYVVVVAATPKAVVFHDPARAPFRVMAVDAFVRRWDAADR
                     WMAIVVPGTKVDEPSSGFPGPPTLGTSESGSRTCTELIDDGIRLAQSNDLDAAERSLT
                     AALSCGGSAPLREMAGLRLLQRRWSDVGETAAAALAIDPGDEHAWDLLATSRFVQDDP
                     IGALDAWNRIDRPRVDLVSVDGLEHTGQRAVERLMGVPGEALLTSRLFTVTRRRLQEL
                     PSAVGTRLEYRPAASGLAEVRAHVVERPLLPFSKSNYLAMGLLAVARSEISASTGSIM
                     GGGERVSGSWRFWPDRPRVNAEIVAPAPWGGLWGVEGFGERQPFTNAGIPTARRAGAV
                     LTLSNWVNPWMRLWARGGVDAWEEHGSFGLVSGGLRAASPRERVVIDVEGSGWSGGDG
                     FASLVAAATVRSSREHRGRVYVARAGAAAATSRTPPDIWFGGDTGRARPTLLRAHPII
                     EDGGLRVEQIGRRIVHLSVEARQWWSTPWPIRVGAALFVDSARVGNRFDANAQGDVDM
                     GLGARLAVPGLDGVFRLDLAKGLRDGGTAFSFVYEP"
     gene            complement(8623..8961)
                     /locus_tag="AC16CY_06335"
     CDS             complement(8623..8961)
                     /locus_tag="AC16CY_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353436.1"
                     /translation="MPRVHAQASHAAAPSAIEAALQQHISSTAAERADVLRVLEHPAV
                     KDVADRAGVDLRRAAGAVATLEGEDLHVLAAQARQAETALAGGQMRIDGPTLIIIALL
                     VVILIIVAVK"
     gene            9110..>9599
                     /locus_tag="AC16CY_06340"
     CDS             9110..>9599
                     /locus_tag="AC16CY_06340"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3353437.1"
                     /translation="MQTDGRAKLNSWVAALERRYLADLRIQEVTRALRALSAAYVEHR
                     GRGTGRHVRGALDTAGKRAAFAMYYAPLHYIAVLEVVRALGADQPAPASIVDLGCGTG
                     AAAAAWAIAGGSTPVVTGIDRHPWAVSEARRTYKELGLHGMARQGDFSESGFKGFKGF
                     TRF"
BASE COUNT         1640 a         3249 c         3176 g         1534 t
ORIGIN      
        1 cacaaccggg cgatcgcgtt catctcccgc cggcacctgc cgctgcggga gaccgatgat
       61 gacttcgatg tcgaggacgg ccgtcccaat cctgaggagg cgctggcgac cgggcagcag
      121 ggacggcgcc tgctcgacgc gattcagcgg ctgccggtga gcctgcggca ggtcgtgacg
      181 ctcgcgctcg aagggctcag ctacgccgag atcggagacg tgctcggcat ttcggaaacc
      241 aacgtgggcg cgcgcctcac gcgcgcgcgt cagagactgc ggcaactgct gctagcccac
      301 aagggcggag gcgagtagag gacgacgatg caggaatcgg atctcgagct cgagggatgg
      361 caacgccagt ggcagtcgca gcccgcctcc atcgacctga ggcagaaggt cgaggcgggc
      421 aaccgcgcca tgcggctcgg cctcatgtcg gaaatcgccg tcacgatcct catggggggc
      481 ggcagcctcc tctgggccat cgtgtcggag cgcgccgaag tgctcgtgct cgccgtcgcc
      541 gtctggggct tcatcacaat cgcctggacg gcttcgattc tgttgcggag gggcctgtgg
      601 caaccggcca ccagcacgac ggcggcgttt ctgaacgtgt ccatcctccg ctgcgagcga
      661 agtctggagg cgatcgtcgt ccaggccgtg ctctatgtcg tgattcttgc cttcgatctg
      721 gcgtggctct actggtaccg gcaggaaacc gacgtctgga cgtttctgac cagacccgtg
      781 gtgctcatcg tcgtggggat cgtcacgccg atcctgggcg ccgtcgcgct gtggtatcga
      841 gggcgcgtgc gccgggagct cgagaacctc cggagactgg ctacttcgct gccccttccc
      901 tgatgaacgt gccgcgattg agctcgtcga acgcctgctg cagctcctgg cgcgtgttca
      961 tcacaatcgg gccgtaccag gcgaccggct cttcgatcgg ctgacccgag acgagaagaa
     1021 acctgatgcc ctcgtcgccc gcctgcaccg tgatctcgtc gccgctgtcg aagagcacga
     1081 gcgagcggtt gccgatgctg tcgggaacgg cctcgccgtc gccgccgatg atgtccgtgc
     1141 ggacgggcct gggcgccgat gcgtcgcgaa agcttccgga gcccgcgaag acatacgcga
     1201 aggcgtggcg cgacgcctcg acgggcaggc tcctgcggac gccggccggc accgacacgt
     1261 cgatatagcg cggatcggcg gccacgccgt cgacgggccc cgacctgccc cagaacgtcc
     1321 cgcacacgat tcgcacgcgc gtcccgtcgt cgtcaacgac ttcgggcacg tccttgccct
     1381 gcacgtcctg gtagcggggc ttggtcatct tcagcgacgc ggggaggttg gcccagagct
     1441 gaaagccgtg catgcggccc tgcgcgtcgc ctttcggcat ctcctggtgg aggatgccgc
     1501 tgcccgccgt catccactgg atgtcgcccg agccgaggct gccgctgttg ccaaggctgt
     1561 caccgtgatc cacggtgccc gcgagcacat aggtgatcgt ctcgatgccg cgatgcggat
     1621 gccaagggaa ccccgcgagg tattcgtggg ggttgtcgtt gcggaagtcg tcgaacagca
     1681 ggaacgggtc gtattcggtc gtgtcgccga atccgaacgc gcggcgcagc ttcacgccgg
     1741 cgccctcgag cgtgggtttg gagagagaca cgcgcttgac gggtcggatg gacacgggac
     1801 ggctccttcg agaaggggaa tccttcgagt ataacgccgc atcggcgcat aatgctctga
     1861 atgcctgagt ccagcgaccg ctcagcgcgg atcgtcatgc ggcgcttcga gtcggctgcc
     1921 gacgccgacc ggcacgacct ggaattctgg atgcagatcc ccgagcggga gcgcgtgctc
     1981 caggtgtggc ggctcagcca ggagatctgg cgccttcgag gcgagcgagg cgatgaaccc
     2041 ggactttgtc gatctgttgc gcgcgttcat cgccgctgac gtccgcttcc tcatcgtcgg
     2101 ggcgtatgcg ctcgcgctgc acggccggcc gcgcgccacc ggcgacctcg atgtctggat
     2161 cgacgcgacg ccggacaacg cagcgcgggt gatgcgtgcg ttgacgtcgt tcggcgcgcc
     2221 gctggccgac gtctcggtgc acgatttcag ccgccctggc gtgacctatc agatcggcgt
     2281 ggcgcccgga cgcatcgacc tcctgaccga gttgaccggt ctgagctttg cggacgcgtg
     2341 gccgggccgc ctgcgccgcc ccttcggcga ggtcgacgtc gacttcatcg gacgggaggc
     2401 cttcattcgc aacaagcgcg cgaccggtcg agcgaaggat ctgggcgaca tcgaggggat
     2461 gtagccgatg atccgctcac aaggtcggag tcgctgtaat tgtccaagca gcgtctcatc
     2521 cgctccaacc aatggtttgc gcccggtgag atcgttcctc tcgaggaaga gctcggccaa
     2581 cgaatgggcg gtctcaacca gattcaactg atcccccatc gattcggcgg cggtattcgc
     2641 gcggatgagc agttccccgc gagcagggtc ttcgacgtcg aaatagtctg ctcgagtcgc
     2701 aagaatactg ggcagcgacc cgtacgtcgc ttcgagcttg tcgagccatt cgagaagtcg
     2761 gcgtcggaga tccggcctct gttcgtcaga tgcgttgaca atatccacag ccagaccatg
     2821 aaccacgtcc caatcttcgg tgctgattcc atcaggcgta ggcatggcgc tctcagacag
     2881 gtcagactga ctacgatgag gggtcttcag cctcacacta acttcgtcgc cgactccgag
     2941 ccctccggcc taagccggac acacgcccgt cacggtccgg ctgaagccgg acactacgtt
     3001 cgataggtgc gagtcgtgtg cgtgcgcagt gtccggcttc agccggacgc agcgaacctg
     3061 aacgtgaaca cgaaccgaga agagagaacc gagaagcgtg aacgacagtc gtcgttcatt
     3121 gcttgacgta gatctccccc gacttcctga actcgaccga cttctcctgc atgccggcgt
     3181 cgcggatctg ctgagtcagc tccatcgagc agaacttggg cccgcacatc gaacagaagt
     3241 gcgcgacctt cgccccttca gcaggaagcg attcgtcgtg atacgagcgc gcggtgaccg
     3301 gatccatcgc caggttgaac tggtcttccc acctgaactc gaaccgtgcc ttcgacagcg
     3361 cgtcgtccca gacctgcgca cgcggatggc ctttggcgag gtcggcggcg tgtgcggcga
     3421 tcttgtacgc gatcacgccc gccttcacgt cgtcgcgatc cggcagcccg aggtgctcct
     3481 tcggcgtgac gtagcagagc atcgccgtgc cgtaccaccc gatcatcgcg gcgccaatcg
     3541 ccgaggtgat gtggtcgtag ccgggcgcca cgtcggtcgt gagcggtccc agcgtgtaga
     3601 acggcgcctc tccgcaccac tcgagctgct tctcgacgtt ctccttgatg agatgcatcg
     3661 ggatgtggcc gggtccttcg ttcatcacct gcacgtcgtg ctcccaggcg atgcgcgtca
     3721 actcgccctg cgtcttcagc tcagcgaact gcgcctcgtc gttggcgtcg gcgatcgatc
     3781 cggggcgaag cccgtcgccg agcgagaacg agacgtcgta cgcgcgcatg atctcgcaga
     3841 tctcggtgaa ccgggtgtag aggaagttct cctggtgatg ggcaaggcac cacttcgcca
     3901 ggatcgaccc gcctcgcgag acgatgcccg tgacccgtct tgcggtcatc gggatgaacc
     3961 gcagcagcac gccggcgtga accgtgaagt aatcgacgcc ctgctcgcac tgctcgatca
     4021 gcgtgtcgcg atacacctcc caggtgaggt cctcgggacg cccaccgact ttttcgagcg
     4081 cctgatagat cggcacggtg ccgatcggca ccgccgagtt gcggatgatc cactcccgcg
     4141 tggcgtggat gttcttgccc gtggacagat ccatcaccgt gtcggcgccc cagagcgtgg
     4201 cccactggag cttttcgacc tcgtcttcga tcgacgagcg cacggccgag ttgccgatgt
     4261 tcgcgttgat cttcacgagg aagttccggc cgatgatcgt cggctcgagc tcgaggtggt
     4321 tgacgttggc gggaatgatc gcgcggccgc gcgcgacctc ggaccgcacg aactccgccg
     4381 gcagcccctc gcgggcggag acgaactcca tttcggcagt gatctcgcct tgacgcgcgt
     4441 agtagagctg cgtgccgacg tgacccctcg cccgccgggc cgcgatccac ggctcgcgca
     4501 gcctgggcag cccctcgcga acgtcgtgcc cctgcggccc gctcgtgtcg tagagacgga
     4561 cggggtgctc gccgcctccg agcgtcacct cgcgcatcgg tacgcgcagg gtgacgggat
     4621 ctccatccgg ggccagggcc gcggtgcgct cctcgaacac ttttcgggag ttgggataag
     4681 ccgagctgaa gtcgatcatc ggaactgctc caatcgggcc ggggcaacct gcggcgggaa
     4741 agcggagggc aacgccgcac tccctacgcc ggtgtcaacc gggtcaggtt ccaagggtct
     4801 gtctcaatcc cgctcagcag gatacccctg gcgactgaag cggattatac ctgtcgttcg
     4861 gtggataatg cgcgtatgcg cattgcggtg ctgctcagtt caatcgttgc agcggccatc
     4921 gaggcgggcg cccagacgac gccagccaag ccgcctgccc agccacaacc cggcacggcg
     4981 caaacaaaac cagcccccgc gccgcaaaca cgcaagccgg ccccgccagc ctcgacgcga
     5041 agcggaatgg cgatcaccgt cgccgatgct cgcggcgtgg tcttgtcagg agtgcgcgtc
     5101 gaagcgatcg ggcccacgat gcgggacgcg gagacgaacg ccagcggcca actcaactat
     5161 cccggcctcc tgccaggggt ctatcggctg aagttttcag gcgagggctg gatcatgttc
     5221 gagcgcgagg tcacactgcg cgcgggacag gtcgccgacg tggacgtcac gctgaacgcg
     5281 gcgccgaagc ccccgcccgt accggagcct gcgccgcagc cggtggtgga ggcgcccgcg
     5341 cacggtccca aaggcgagac acagacgatc ggcatcgagg gactgctcga gaaggagttc
     5401 atcggcaagg acccgcgccg tgaatccctc ctctcgtgca gcggcaacga acgggccacg
     5461 atgatccagc tcaatggccc catgccccag cggctgtacg aaaacggaga tgcggtgtat
     5521 tacgtgctgg gaggcgacgg cacggccacg atcgacgggc gcaagtcccc ggtcaggacc
     5581 aacgacttcg tctccgtgcc tcgcggctcg gctcacgcgt tcgaccggac gggaaaacgc
     5641 gccctcgtgc tgctggccgt gctgagcggc gagccgtgcg agcgggccaa gtagcacgct
     5701 ctcggcgggc ccggggagag agctccctca gcgagttcgg gccgcgccgg caccatcgaa
     5761 cggatagcca acgaagttcg gcgcctgctc cgggcgcagc agcagcgtcg tgaacatgaa
     5821 cgccattgcg tagcggccgt cgccgagccg cgtgacgttc aacggctgct gaaacaccac
     5881 gtgccagtcg tagacgttgt cccagacgtc gagaccgatc tcgatgaaat ccggatactt
     5941 gtgcgtggcc gccaaccacg tccgctgctg ctgccgcaca cgcgagacgc ctcgccactt
     6001 gctcgtatac acgtcgtcga actgccgctc gaaagtctcc cgcagttgat tgatggccgc
     6061 ggccgactgg gcgtcgccat ccgcggcgcc agccgcaccc ggcgcgacca gggcggccag
     6121 gcccgcgatc actcccccgc caacgagatc acgtcgcgga accatctcgt cctcctgtca
     6181 tggagtcgtg accatcagcg tggcgcggta gtcccccggc gccgtggtcc ggctgctgaa
     6241 cggaaacggc tgcaaatcga tcagggtgat acgggccccc gccgcaatga cgctggcgcg
     6301 ggaagagtcg ccggtgtgaa gttcgtggaa ggccgaggcg ctcgcgtcgt ccacgacgcg
     6361 tactcgcacg atggcgtcgc cgccctggat gcagaccgca tccgccgggc atcgcgagtc
     6421 gcccgtgacg tggacgaact ggactcgaag gcccgcctcg tccacgaaag ccgtctggcc
     6481 tggggccagg gtgaaacgct cgttcagctg gaccgtcgga ccgactggac tgttttcgtc
     6541 acacgttgtg accgacagga gacaaaccag cagcccaata gcgcgcacgg gccaggcgat
     6601 agcgaagaac gtaccacctc gatggggtca gggttcgtag acgaacgaga aggccgtccc
     6661 accgtcgcga agacccttgg ccaggtcgag ccgaaacacg ccatccaggc ccggcacggc
     6721 gagcctcgcg ccaagcccca tatcgacgtc cccttgcgcg ttggcgtcga accggttgcc
     6781 gacacgcgcg gagtcgacaa agagcgccgc gccgacacga atcggccacg gcgtgctcca
     6841 ccactgccgc gcctcgacgg agaggtgcac gatgcgacgt ccgatctgct ccactcgcag
     6901 cccgccatcc tcgatgatcg ggtgcgcgcg aagcagcgtg ggccgcgcgc ggcccgtgtc
     6961 gcctccaaac cagatgtcgg gcggcgtgcg tgaggtggcc gccgcggcgc ctgctctcgc
     7021 gacatacaca cgcccacggt gttcccgtga cgagcgcacg gtcgcggcgg ccacgagcga
     7081 cgcgaagccg tcgccgccgc tccatcccga gccttcgacg tcgatcacga cgcgctcacg
     7141 cggcgaggcc gctcgcagtc cgcccgagac cagcccgaag cttccgtgtt cctcccatgc
     7201 atcgacgccg ccgcgcgccc acagcctcat ccagggattg acccagttgg agagcgttag
     7261 gaccgcaccg gcacgacggg ccgtgggaat gccggcattg gtaaacggtt gacgctctcc
     7321 aaagccctcc acaccccaga gaccgcccca gggcgccggc gcgacgatct cagcattcac
     7381 tcgaggacgg tcgggccaga agcgccagga cccgctcacg cgttcgccgc ctcccataat
     7441 cgatccggtt gacgcgctga tctcgcttcg cgccacagcg agcaggccca tcgccaggta
     7501 gttcgatttc gagaatggca ggagcggccg ctccaccacg tgagcgcgca cttcggccaa
     7561 cccggacgcg gccggccggt actcgaggcg ggtgccgacg gctgacggca gctcctgcag
     7621 ccggcgcctg gtcacggtga acaatcggga cgtaagtagg gcttccccgg gcacgcccat
     7681 caatcgctcg accgcgcgct gcccggtgtg ttcgagaccg tccaccgaca ccaggtccac
     7741 ccggggccga tcgatgcggt tccacgcgtc gagcgccccg atgggatcgt cctggacgaa
     7801 gcggctggtg gcgagcaggt cccacgcgtg ctcgtcgcca gggtcgatcg ccagtgccgc
     7861 cgccgccgtc tcgccgacgt cggaccagcg ccgctgaagc agccgcaggc ccgccatctc
     7921 gcgcagcggc gccgatccgc cgcacgacag cgcggccgtc aggctgcgtt cggcggcgtc
     7981 gagatcgttc gattgcgcaa gccggattcc gtcgtcgatg agctcggtac aggtccggct
     8041 tccgctttcg ctcgtcccga gcgtcggcgg cccgggaaag ccggatgacg gttcgtcaac
     8101 cttcgtcccc ggaaccacga tggccatcca ccgatcggcg gcgtcccacc gacgtacgaa
     8161 cgcgtcgacg gccatgaccc tgaatggagc gcgggccgga tcgtgaaaca cgaccgcctt
     8221 gggcgtggcc gcgaccacga cgacgtagtg gaaggtccct ggacggtctt ggatcagggc
     8281 gagaaccgga cggcctcgat ccaactcggc cgacatgcgc tggccggtgc cttcgaggcc
     8341 cgctacgctc cagccgcgcg cgcgcaggtc gccgaccagc cgatcggtcc gaatgcctcc
     8401 cgcgctgcga tcgaccagat gcgcgaacga ttcggcgtcg agcccccgct caccccagta
     8461 gcgcaggatc atcgcggcgg cggcgccgcc gcacagcgcc tcggactgcg agacgaaagg
     8521 gacgtcgagg atcggcagcg gccctggagc tggctgggca tcggcatgcc cagccagcag
     8581 gacgaagaca acgagaccgg acgcgcgcac gcggcccgcg agctatttga cggccacgat
     8641 gatcaggatc acgacgagca gggcaatgat gatgagcgtc ggtccgtcga tgcgcatctg
     8701 cccgcccgcc agcgccgtct cggcctgccg cgcctgagcg gcgagcacgt gaaggtcctc
     8761 gccctcgagg gtcgccacgg ccccggcggc gcgacgcaga tcgactcccg cccgatcggc
     8821 gacatccttg accgcggggt gttcgagtac gcgaagaaca tctgcacgtt cggcagcggt
     8881 gctggagatg tgctgctgca gcgcagcctc gatcgccgac ggagcagcgg cgtgcgaggc
     8941 ttgagcatgg acccggggca tcgccgtgag tactgtcaac aggattgcca aaccaacaac
     9001 gggtaccctt gccgaggggt cagacccctt cgcatgcttg caccgatcaa cgagcgtgtt
     9061 catgtgctgg tgccctcctc aagcgaccat tgtagcgaag gcatcgccaa tgcagaccga
     9121 cggtcgagcg aaactgaaca gctgggtcgc cgctctcgag cgcaggtacc tggccgacct
     9181 ccgaatacag gaggtcaccc gggcgctgcg ggcgctgtcg gccgcctacg tcgagcaccg
     9241 cggccgcggg accggcaggc acgtgcgcgg cgctctcgac accgccggaa agcgggccgc
     9301 gttcgcgatg tactacgcac cgctgcacta catcgcggtg ctcgaagtcg tacgcgcgct
     9361 cggcgctgac cagcccgcgc cggcctcgat cgtggacctg ggctgcggca cgggagcagc
     9421 ggcggcagcg tgggcgatcg cgggcggctc gacgccagtg gtcacaggta tcgaccggca
     9481 tccctgggcg gtctcggaag cgcgccgcac ctacaaggag ctcggactgc atgggatggc
     9541 gcggcagggg gacttcagtg aatcagggtt caaggggttc aaagggttca cgaggttca
//
LOCUS       DBNGSH010000270         1827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4606553, whole genome shotgun sequence.
ACCESSION   DBNGSH010000270 DBNGSH010000000
VERSION     DBNGSH010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4606553"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1515
                     /locus_tag="AC16CY_06345"
                     /pseudo
     CDS             124..1515
                     /locus_tag="AC16CY_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580727.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
     gene            1518..>1827
                     /locus_tag="AC16CY_06350"
     CDS             1518..>1827
                     /locus_tag="AC16CY_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353438.1"
                     /translation="MTAVVAAAMLSAQTAIPTREGRAGGQGQTQGRRGGGPAPEVNLP
                     SSPVTTPLPAISAEITGPGPIFQSLMELKPADDMAHFKYEAREYFVSGTANGQPYTTR
                     I"
BASE COUNT          342 a          607 c          626 g          252 t
ORIGIN      
        1 cgtctcgatt ccgatggcac ccgaagtgga ctcgctgaat gtcgcgactg ccggagccat
       61 cctcgtctat gctgcacgac ggctatcgac ccgaaaggcg tcaggcgtca ggcgaaaggc
      121 ccaatgagca gcagcaacct cttcgacgac gaggacggcg aaccgagggc cggcgagcga
      181 ccacgcacgt ccactcacgt cgaaagcggg acgcccctgg ccgagcggat ccgtccacga
      241 acgctcgacg agatcgtcgg gcaggaggac atcctgagcc ccggcaagcc gctgcgcgag
      301 gcgatcgagc gggacctgct gcagtcgatc atcttctggg gtccgccggg aacgggcaag
      361 acgacgatcg cgcgcgtcat cgcggagttg accgaggcgc acttcgtgcc gttcagcgcc
      421 gtgctgtcgg ggatcaaaga gatcaaggag gtcatggccg cggcccagga ccgccggcgg
      481 cggaccgggc ggcggacgat cgtcttcgtg gacgagatcc accgcttcaa caaggcgcag
      541 caggacgcct tccttccacg cgtcgaggcg ggagacatcg tgctgatcgg cgcgaccacg
      601 gagaacccgt ctttcgaggt caatgccgcg ctcctctcgc gctcgaaggt gttcgtgctg
      661 cggccgctta cgagcgaggc cgtcgaacgg atcctcgggc gcgcgctcgc ggatcccgag
      721 cgtgggctgg gcgccagcgg catccaggtc gcggacgagg ccgtcgcggc gatcgcacgc
      781 ttcgccaacg gcgacgcgcg cgcggcgctg aatctcctgg agttcgctgc tgcgtccgcg
      841 ccggtggacc caaggacggg ggccaggcaa ctcggcctgg cgcagctcga gcactcgatc
      901 cagcggcggg cgctgctgta cgacaaagac ggagaggagc actacaacct gatctccgcg
      961 ctccacaagt cgatgcgcaa cagcgatccc gatgcagcgg tctactgggt ggcccgcatg
     1021 ctcgaggcgg gcgaggatcc gctctacatc gcgcggcgcc tggtccggtt cgcgtcggaa
     1081 gacgtgggca atgccgaccc tcaggcgctg acggtcgccg tcgccgcaaa agacgcggcg
     1141 cacttcatcg gcatgccgga aggcaacacg gcgctggccc aggcggcgat ctacctggca
     1201 accgcgccca agagcaatgc cgtctatacc gcgtacacgt cggcggccgc agacgcgcac
     1261 actcaagcgg ccgatccgtt gacgctgcat ttgcggaatg ccccgacccg gctgatgtag
     1321 caactggact acggcaaggg ctaccagtac gcgcacgacg agcccgacgc cgtcacgggg
     1381 atggagtgcc tgcctccatc gctggccggc cggacctact accgccccac ggaacgcgga
     1441 ttcgagaagg agatcaagcg gaggctggac ttatgggaag aacggaaaaa ggtcaagggt
     1501 tcgccggagc gctgaccgtg accgcggtcg tggcggccgc gatgctttcg gcgcaaacgg
     1561 caattccaac gcgcgaaggg cgcgccggcg gacagggtca aacgcaaggc cggcggggcg
     1621 gagggccggc gccggaggtc aacctgcctt cgagcccggt gacgactccc ctgccggcga
     1681 tctccgccga gatcacgggg ccgggtccga tattccagtc gttgatggag ctcaagcccg
     1741 ccgacgacat ggcgcacttc aagtacgagg ctcgcgaata cttcgtctcg ggtacggcca
     1801 acggtcaacc ctacacgacc cgaatcg
//
LOCUS       DBNGSH010000271         3946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4266292, whole genome shotgun sequence.
ACCESSION   DBNGSH010000271 DBNGSH010000000
VERSION     DBNGSH010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4266292"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            755..1681
                     /locus_tag="AC16CY_06355"
     CDS             755..1681
                     /locus_tag="AC16CY_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3353439.1"
                     /translation="MRQLMLACCTATVIAAGVLTAPRPLAQSFGAPAGTVPAPDPNVP
                     LYFEVASVKPSDPKANPNMVAIRRAPGGRFDTVNSPVRMLITFAYQIQTFQLIDAPSW
                     VNEERYNIIAKMEGDPPAIIPGSGADHMMLAMRSLLADRFKLAVRRETRELDIYALTM
                     ARPGGKPGPALKPASGDCSPEAFAARRGAPPPAPGGPPPVVCGIQQGPGRIRFGGFPL
                     TLFASNISNRVGRQVVDRTGLTGNWDFEMTFAPEAAPGALPPGADAAPPLDPDAPNFF
                     TAVQEQLGLKLDATKGPVDVLVVERIERPTPD"
     gene            1807..>3946
                     /locus_tag="AC16CY_06360"
     CDS             1807..>3946
                     /locus_tag="AC16CY_06360"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3353440.1"
                     /translation="MRVIGRLSVVLACVLYAQALVPTLAHAQATVAGVVRDTSGAVLP
                     GATVEATSDVLIEKVRSAVSDSTGQYRITDLPPGTYNLTFALPGFNAVARQGVQVSGT
                     GVIPINVEMRVGAIQETVTVTGETPLVDTQSTRRESVLSSDVIAALPATRTYGALLSA
                     IPGLMTNNSSLSAMATPDMTFFSAHGGRGNEGRVHIDGLPVAASFNGGGVSTFTYDTV
                     NAEEMQVLVSGGLGEAEAGGPSINLVPRSGGNAFSGSAFYNSAGEWSSGNNITPYLDS
                     IGITEPPAIVKLWDVSASVGGPIMRDRLWFFGSARQYGNQQLAEGLYANRYAGDASHW
                     DYATDTSVLGRTANSRDIGQFRLTAQVSERNRVSFSHEYQHRCTGSSLSADGQACRAR
                     GDDWISNGSRAFGSPEAFPGYHDTPYNVTQATWSSPLSSRILLEAGFSRFQYIWYGFG
                     QASPDRINLIPVSETLARDGHPANFTYRGIYDPFEIAFADNDANPNNWRGTASYVTGS
                     HNVKVGYQGSYQRSLLGREANTTLLRYSANTPLGTLGPAFNGVSYYIAPRWEQNDRTA
                     TASLFAQDQWTFGRWTVQGALRYDRAWSWSPAEHNGTDVIGIAQDQTKPFLTAPISFD
                     RTVSVDAYNDITMRLGTAWDIFGNGKTSLKVNLGKYLQNATNDENYVANNPARRIVTR
                     VGGLGQPARGWADGNNNRIVDCDLTNPDEQN"
BASE COUNT          674 a         1351 c         1289 g          632 t
ORIGIN      
        1 gacgtcatcg ccgccacgtg gctcacgctg atccgagctc cgcgtatgtg ctcaccacgg
       61 gcaggcatgc cggctggcgg ccgcgggcgc tgcggccggg aaaccggctc tccgctcata
      121 ccctgccgcc gccacgtcga ctaaacgctg tctcgagaca tggtccgtta cgcggcgcca
      181 aacgtccgtg ttgccacgcg cttcccaaac gcgtggacgg aagtcgacgt gaggaatcgc
      241 gtcgcccggt ttccggcttc gcagcgtggt cggccttgct tacgcgtccg ccatccggca
      301 cgcctgctcg tggtgaggaa gcgcggccgt gagagccatg cgcgaggaga cacagaccgg
      361 gccacgagcc gcgtacgcca ggccgagcgc accgcgcagc aggaaagtgt ccggcgctat
      421 tgcaccccag cgcgcaggcg tgcgcgccgg ggaccccgcg cctgccgcgc gtcttccgcc
      481 atcgcgttcg agttgcagcg agatgccctg gacaacttga gccgcgcagc ggatgtcgcc
      541 tcgcgttagc gaatagacgc gcggggagcg aaagagtccg agcgccgggc attttcccgg
      601 ccgccgcgtc cgcggtcgtg gaacggtctg cgtctccgct gcgcagcgac gccacggctg
      661 cgagcggtgc ggacccacta aacaaagacg ttgggagttg agccaacttc tggaccggtt
      721 ctggtgtcaa atggctagcc acgtggagtc aacgatgcga caactgatgc ttgcgtgctg
      781 cacggcaaca gtcattgccg ccggcgtcct caccgcgccg cggccgttgg cgcagagctt
      841 cggggccccg gcgggaacgg tcccagcgcc cgatccgaac gtcccgctct atttcgaggt
      901 cgcctccgtg aagccgagcg accccaaggc caacccgaac atggtggcca tccggcgcgc
      961 gccgggcggg aggttcgaca ccgtcaattc gccggtccgg atgctgatca cgtttgccta
     1021 tcagattcag acatttcagt tgatcgacgc gcccagctgg gtcaatgagg agcgctacaa
     1081 catcatcgcc aagatggagg gtgatcctcc ggcgatcatt cccggatcgg gcgccgatca
     1141 catgatgctg gcgatgcgct cgctgctcgc cgatcggttc aagctcgccg tccggcgcga
     1201 gacgcgggag ctcgacatct atgccctgac gatggcgcgg ccgggcggca aaccggggcc
     1261 cgctctcaag cccgcgtcgg gagactgctc gcccgaggcg tttgcggcgc gccgcggcgc
     1321 tccgcccccg gcacccggcg gcccgccgcc cgtcgtctgc gggatacagc aggggccggg
     1381 ccggatccgt ttcggcggat tcccgctcac gctgttcgcg tcgaacatct cgaaccgcgt
     1441 cgggcgacag gtcgtcgatc gcaccggtct gacggggaac tgggacttcg aaatgacctt
     1501 cgctcccgag gctgcgcctg gagcactccc tccaggcgca gatgcggcac cgccgttgga
     1561 tccagacgcc ccgaacttct tcaccgcggt tcaggagcag ctcgggctga agctcgacgc
     1621 gacgaagggc ccggttgacg tgctggtcgt ggagcgcatc gagcggccga cgcccgacta
     1681 atctcccgcc ttgacatccc gcagaccgga gttcaagatc cgcccacaat cgaacagcca
     1741 tatcagccgg ctatagcgga ggcgtcggaa cccccggtgc aagtccatct ttcctgagga
     1801 gggttcatgc gtgtaatcgg cagattgtcg gtggttcttg cctgtgtgct ctacgcgcag
     1861 gcgctcgtac ccacgctggc tcacgcgcag gcgacggtgg ctggcgtggt ccgggacacg
     1921 tccggcgcgg tgctgccggg cgccacggtc gaggcaacga gcgatgtctt gatcgaaaag
     1981 gtccggtcgg ctgtcagcga cagcaccggt cagtaccgca tcacggatct gccaccgggc
     2041 acctacaacc tcactttcgc gcttccgggg ttcaacgccg tcgcccgcca gggcgttcag
     2101 gtgtcaggaa ccggcgtcat cccgatcaac gtcgagatgc gggtcggcgc gatccaggag
     2161 acggtcaccg tcaccggcga gacgccgctg gtcgacacgc agtcgacgcg ccgcgaatcc
     2221 gtgctgagtt cggacgtcat cgcggcgttg cccgccacgc ggacgtatgg cgcgctgctt
     2281 tcggcgattc caggtctgat gacgaacaac agctcgctca gcgcgatggc gacgcccgac
     2341 atgacgttct tctcggcgca cggaggccgg ggcaacgagg ggcgcgtgca catcgacggc
     2401 cttcccgtcg ccgcctcctt caacggcggc ggcgtgtcga cgttcaccta tgacacggtg
     2461 aatgccgagg agatgcaggt gctcgtctcg ggcggtctgg gcgaagccga ggccggcggt
     2521 ccgagcatca acctcgtgcc gcgttcgggc ggcaacgcct tcagcggctc cgcgttctac
     2581 aactccgcgg gcgagtggtc atcgggcaac aacatcacgc cgtacctgga cagcatcggc
     2641 attacggaac cacccgcgat cgtcaagctg tgggacgtga gcgccagcgt cggcggtccc
     2701 atcatgcggg accggctgtg gttcttcggc agcgcgcgac agtacggcaa ccagcagctt
     2761 gccgaaggcc tgtatgcgaa ccgctacgcc ggcgacgcga gccactggga ctacgccacc
     2821 gacacgagcg tcctcggacg cacagcgaac tcgcgcgaca tcgggcagtt caggctgacg
     2881 gcgcaggtgt cggagcggaa ccgcgtctcg ttctctcacg agtaccagca ccggtgcacc
     2941 gggtcgtcgc tgtcggcgga cggccaggcc tgcagggctc gcggcgacga ctggatcagc
     3001 aatggaagcc gggcgttcgg ttcaccggag gcgtttcccg gctaccatga cacgccgtac
     3061 aacgtcacgc aggcgacctg gtcgtcgccg ctctcgagcc gcatcctgct cgaggccggg
     3121 ttctcccgct tccagtacat ctggtacggc ttcggccagg cgtccccgga tcgcatcaac
     3181 ctgatccccg tgtcggagac gctggccagg gacggtcatc cggccaactt cacctaccgc
     3241 gggatctacg acccgttcga gatcgccttc gccgacaacg acgcgaaccc gaacaactgg
     3301 cgcgggacgg cgtcctacgt cacgggctcg cacaacgtca aggtcggcta tcagggctcg
     3361 tatcagcggt cgctgctcgg ccgcgaagcc aatacgacgc tgctgcgcta ttcagcgaac
     3421 actcccctcg gcactctcgg ccccgccttc aacggcgtgt cgtattacat cgcgccgcgc
     3481 tgggagcaga acgatcgcac ggcgacggcg tcgctcttcg cgcaggatca gtggacgttc
     3541 ggacggtgga cggttcaggg cgctctccgg tacgaccggg cctggagttg gtcgccggcc
     3601 gagcacaacg gcaccgacgt gatcgggatc gcgcaggatc agaccaagcc gttcctgacg
     3661 gcacccatct cgttcgatcg taccgtcagc gtggatgcct acaacgacat cacgatgcgt
     3721 ctgggtacgg cctgggacat cttcggcaac ggcaagacgt cgctgaaggt aaacctcggc
     3781 aagtacctcc agaacgccac caacgacgag aactacgtgg ccaacaaccc cgcccgccgc
     3841 atcgtcacgc gcgtcggcgg gttgggccag cccgcccgtg gctgggcgga cggcaacaac
     3901 aaccgtatcg tggactgcga tctgaccaac cctgacgagc agaaca
//
LOCUS       DBNGSH010000272         2938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3247442, whole genome shotgun sequence.
ACCESSION   DBNGSH010000272 DBNGSH010000000
VERSION     DBNGSH010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3247442"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /gene="tssC"
                     /locus_tag="AC16CY_06365"
     CDS             complement(<1..1315)
                     /gene="tssC"
                     /locus_tag="AC16CY_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HIR3353441.1"
                     /translation="MADAKSQRDAQSAAGALEVTDLDALLKKEFKPKTDEAKEAVERA
                     VRTLAEQALSQTKLIGADVVKSIEAIIAQLDKKLTEQINLILHHPDFQKLEGGWRGLH
                     YLVSNTETDEMLKIRFMNISKTDLGKTLKRFKGTAWDQSPLFKKVYEAEYGQFGGEPF
                     GCLVGDYYFDQTPPDTELLGEMAKVASAAHTPFISGASPTVMQMDSWQELANPRDLTK
                     IFTTPEYASWRSLRESEDARYLGLAMPRYLSRLPYGAKTSPVEEFDFEEDTGAADHSR
                     YTWANAAYAMAVNINRSFKLYGWCSRIRGIESGGSVEGLPTHTFPTDDGGVDMKCPTE
                     IAISDRREAELAKNGFMPLVHRKNSDFAAFIGAQSLQKPFEYDDPDATANANLAARLP
                     YLFACCRFAHYLKCIVRDKIGSFKERDDMQVWLQKWIMNYVDGDPA"
     gene            complement(1350..1859)
                     /gene="tssB"
                     /locus_tag="AC16CY_06370"
     CDS             complement(1350..1859)
                     /gene="tssB"
                     /locus_tag="AC16CY_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HIR3353442.1"
                     /translation="MAKASSQKFIARNRAPRVQIEYDVELYGAEKKIQLPFVMGVLAD
                     LSGKPAEPLAPVADRKFLDIDVDNFDSRMKALKPRASFQVPNTLTGEGSINVELTFDS
                     MDDFSPAAVARKVDALSKLLEAREQLANLITYMDGKTGAEELVAKLLNDPALLQSLAT
                     TKKAEEPAS"
     gene            complement(1886..>2938)
                     /gene="tssA"
                     /locus_tag="AC16CY_06375"
     CDS             complement(1886..>2938)
                     /gene="tssA"
                     /locus_tag="AC16CY_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HIR3353443.1"
                     /translation="CDRRACCGPGPRMNARWDGHGLLEPISADQPCGKNLEDTDTLGA
                     FDAYQLFGQSSLEPEPGETRKNRPPDWAEIKERALDTLRTSKDLRLLAHLGTALLRTD
                     GVPAFAETIGVASRWLDEYWGQVYPLVDEDAILRRNALNCFADPVAVVDGLRRAPLVS
                     SRQHGRFSMRDLDIASGQLMPGEADARPDEAQIRAAFGDTPLEELKGLREVVAGALTS
                     LGAIDATMRREAGVEAAPNVDSLQAQLKKMDAALRAQLVLHPNASAEEMGAESSGDGA
                     GTVVAVGAIKSRQDAIRALEAAAEFFRRNEPSSPIPLFLERAKRLVSKDFLEVLADVA
                     PDALPQARSAGGLRDE"
BASE COUNT          445 a         1002 c          927 g          564 t
ORIGIN      
        1 gcgccgggtc gccgtcgacg tagttcatga tccacttctg cagccacacc tgcatgtcgt
       61 cccgctcctt gaaggagccg atcttgtcgc ggacgatgca cttcaggtaa tgggcgaacc
      121 tgcagcaggc gaacaggtat ggcaggcgcg cggccaggtt ggcgttggcg gtggcgtcgg
      181 ggtcgtcgta ctcgaacggc ttctgcagcg actgcgcgcc aatgaaggcg gcgaagtcgg
      241 agttcttgcg gtggacgagc ggcatgaagc cgttcttggc gagctccgcc tcgcgccggt
      301 cgctgatcgc aatctcggtc gggcatttca tgtccacgcc gccgtcgtcg gtcgggaagg
      361 tatgcgtcgg caggccttcg accgagccac cggactcgat gccgcggata cgcgagcacc
      421 agccgtacag cttgaatgac cggttgatgt tgaccgccat cgcgtatgcg gcgttcgccc
      481 aggtgtaccg gctatggtcg gcagcgcccg tgtcctcctc gaagtcgaac tcctcgacgg
      541 ggctggtctt ggccccgtag ggcagacgcg acagatagcg cggcatggcg aggccgaggt
      601 aacgggcgtc ctccgactcg cggagcgaac gccacgacgc gtactcgggt gtcgtgaaga
      661 tcttggtgag atcccgcggg ttcgccagct cctgccagct gtccatctgc atgaccgtcg
      721 gcgaggcgcc cgagatgaag ggcgtgtgcg ccgcggacgc gaccttggcc atctctccga
      781 gcagctcggt gtcgggaggc gtctggtcga agtagtagtc gccgaccagg cagccgaacg
      841 gctcgccgcc gaactggccg tactcggcct cgtacacttt cttgaacagc ggactctggt
      901 cccaggcggt gcccttgaat cgcttcagcg tcttgccgag atcggtcttg ctgatgttca
      961 tgaagcggat cttgagcatc tcgtcggtct cggtgttgct gacgagatag tgcagacccc
     1021 gccacccacc ctccagcttc tggaagtccg ggtgatgcag gatgagattg atctgctcgg
     1081 tcagcttctt gtccagctgc gcgatgatcg cctcgatcga cttgacgaca tcggcgccaa
     1141 tcagcttcgt ctgcgagagc gcctgctcgg ccagcgtgcg gacggcccgc tcgacggctt
     1201 ccttggcctc gtcggtcttg ggcttgaact ctttcttgag cagcgcatcc agatcggtga
     1261 cctcgagggc cccggccgcc gattgtgcgt ctcgctgcga ttttgcgtca gccatggctt
     1321 ttcttttctc cgctcggggg attcacgggt taggaggcgg gctcctccgc cttcttcgtc
     1381 gtcgccagcg actgcaacag ggcaggatcg ttcaggagct tggccacgag ctcctccgcc
     1441 ccggtcttgc cgtccatgta cgtaatcaag ttcgcgagct gctcccgagc ttcgaggagc
     1501 ttgctcaggg cgtccacttt ccgcgcgacc gccgcgggcg agaagtcgtc catgctgtcg
     1561 aacgtgagct ccacgttgat gctgccctcg cccgtcagcg tgttcggcac ctggaagctc
     1621 gcgcgcggct tcagcgcctt catgcggctg tcgaagttgt ccacgtcgat gtccaggaac
     1681 ttgcgatcgg cgaccggagc cagcggctcc gccggcttgc cggagaggtc ggccagcacg
     1741 cccatgacga agggcagctg aatcttcttc tcggcgccgt acaactccac gtcgtactcg
     1801 atctgcacgc gcggcgcgcg atttctcgcg atgaacttct gactgcttgc cttagccacg
     1861 gctcacctcc tggccgttct cacggttact cgtccctcag gccgcccgca gaccgcgcct
     1921 gcggaagcgc gtccggcgcg acatcggcca gcacctccag aaagtccttc gacaccagcc
     1981 gcttggcccg ctcgaggaac agcgggatcg ggctcgacgg ctcgtttcgc cggaagaact
     2041 cggctgccgc ctcgagcgcc cgtatcgcat cctgacgcga cttgatcgcc ccgacggcca
     2101 cgaccgtccc tgccccgtcc ccgctcgact cggcgcccat ctcttctgcg ctcgcattcg
     2161 ggtgcaggac cagctgtgcc cgaagcgcag cgtccatctt cttcagctgc gcctgcagcg
     2221 agtccacgtt cggcgccgct tccaccccgg cctcgcgccg catggtcgcg tcgatggcgc
     2281 cgagcgaggt caaggccccg gccacgacct ctcgcagacc cttcaactcc tcgagcggcg
     2341 tgtctccaaa cgccgcgcga atctgcgcct cgtcgggccg cgcatcggcc tcccccggca
     2401 tcagctgccc ggacgcgatg tccaggtcgc gcatgctgaa ccggccgtgc tgacggctgc
     2461 tgacgagcgg cgcacgacgg agtccgtcaa cgaccgcgac cgggtctgca aaacagttca
     2521 acgcgttgcg acgaagaatc gcgtcttcat cgaccagcgg gtaaacctgc ccccagtact
     2581 cgtcgagcca tctggacgcg acgccgatcg tctcggcgaa tgccggcaca ccgtccgtcc
     2641 tgagaagcgc cgttcccagg tgagccagca gccgcaggtc cttgctcgtc ctcaacgtgt
     2701 cgagcgcacg ctccttgatt tctgcccagt cgggcggtcg attcttccgg gtttcgccgg
     2761 gctcgggctc gagcgaggac tggccgaaga gttgataggc gtcgaaagcc cccagcgtgt
     2821 cggtgtcctc caggttcttg ccgcacggtt gatccgccga aatgggctcg agcaggccgt
     2881 gcccgtccca acgggcgttc atacgcggcc ccgggccgca gcacgcgcgg cgatcaca
//
LOCUS       DBNGSH010000273         6224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2908266, whole genome shotgun sequence.
ACCESSION   DBNGSH010000273 DBNGSH010000000
VERSION     DBNGSH010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2908266"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="AC16CY_06380"
     CDS             <1..971
                     /locus_tag="AC16CY_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR3353444.1"
                     /translation="VTTLALLIGWGAITVSRAGVGIPLTPPADAAAVSPAAARTWQDI
                     VLDTFPENIGRAVVEAEMLQIVVFSVLFGIALALLPEDKRRPMMAFAESLAETMFSFT
                     KIVMLMAPIGVGAAIAYTVGQLGLGVLSNLLQLLATFYVAVGLFVVGVLLPIALIAKV
                     PIRRFAEAVAEPVSVAFATTSSEAALPRAMEAMERFGVPRPIVAFVMPTGYSFNLDGS
                     TLYLSLAAIFVAQAAGIDLSVGQQFVILLTLMLSSKGVAGVPRGALVILLGTVSSFGL
                     PAEPVFVLLGIDAVLDMLRTSVNVLGNCLATVVIAKWEGEPLKTSG"
     gene            complement(988..1773)
                     /locus_tag="AC16CY_06385"
     CDS             complement(988..1773)
                     /locus_tag="AC16CY_06385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HIR3353445.1"
                     /translation="MSWRVNAERIVLLGWGRAILLQLAHPLVAAGVFGHSSFRATPWA
                     AASRLYHTVHAMLALTFGSDADRERTLDAIRAIHRRVNGVLEDGVGPYPAGTRYSAED
                     PALVVWVHATLIESVILAYERLVAPLSAEERDAYCEDAFPIAVALAARPQDVPRRWDD
                     VRSVMERMLASGALAVGSHGRELSRAVLSPAGGWAVAPATWVNRVVTIGLLPSGIRQQ
                     YGLAWAGRDERRLERILSCLRATRRVLPDALALWPEARRNRRP"
     gene            complement(1866..2222)
                     /locus_tag="AC16CY_06390"
     CDS             complement(1866..2222)
                     /locus_tag="AC16CY_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353446.1"
                     /translation="MQLRTARLCLDCDEVHDAQQCPLCASETFAYLTRWVPVPERPNN
                     ASRPADVDAPETVGAYRAMLHPAERPRVGVWRTVRRGAVGLALFGIAGWALRHGGRNH
                     PAGQPQNQDAQGQPQS"
     gene            complement(2239..3141)
                     /locus_tag="AC16CY_06395"
     CDS             complement(2239..3141)
                     /locus_tag="AC16CY_06395"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3353447.1"
                     /translation="MPPEVIVRDWREADPADTQVLYDREQEYWVRELAWDASSAWREI
                     EQARVAGDLPGFIARDVERTVRGWSYFLREGETAHLGAVVAEMPEATGALLDECLAAA
                     LRPSRPARVSCFLPSRAEHFEMALRDRGFACERYHYLSRPITADEGAAESGAWSDGDL
                     TPAAELLRESYGSDGRHFAPHGTPEEWEHYVRSLVERPGCGVMEPAITRVVRGDRGLD
                     ALVLVTRISPGTLHLAQVAVRPSRRREGLARRLVEEIFRVGSARGARQVTLLVGDRNT
                     AAQALYARMGFTARSAFVAGRLEL"
     gene            complement(3205..3801)
                     /locus_tag="AC16CY_06400"
     CDS             complement(3205..3801)
                     /locus_tag="AC16CY_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353448.1"
                     /translation="MQRRLSMSLAVAAAICASTPAWAQVGDARPNVSTFDGTGAHAWD
                     GNVRASDAELVALLAEGVKRSPTLKALVERLNKSDVILYVRMDVTSKTGTGRLTFLTA
                     NAGYRYLVVHVPNGQSKAKQLAALGHDMQHAIVVADAPTVTDSASFRREFEKIGSVSQ
                     SQNGRDLSFASPAAVETKNRIQRELSGDAPAQKVASAN"
     gene            complement(4082..4786)
                     /locus_tag="AC16CY_06405"
     CDS             complement(4082..4786)
                     /locus_tag="AC16CY_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3353449.1"
                     /translation="MSAPRILMIEDEPALTRGVSDALAAEGFEVRVAHDGQIGLDRAL
                     EGVADLILLDVMLPKVNGYEICRAVRTRGLDVPILMLTAKGQEQDVVLGLNLGADDYV
                     TKPFRIGELIARIRAFLRRRAPERDVFRFGQVQVDLKGRKVLSNGAALDLTAKEFRLL
                     AYFVSRPGCALSRDAILDAVWGSSVFVTPRSIDRCVATLRAKIEPNPHHPIYIQTIRE
                     IGYRFEPDGSPALNAS"
     gene            complement(4783..>6224)
                     /locus_tag="AC16CY_06410"
     CDS             complement(4783..>6224)
                     /locus_tag="AC16CY_06410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3353450.1"
                     /translation="AATRLTHTNTPEQDFARLVLDEIGDGVVMLDSDGGIAYPQPAAA
                     RIPNPESRIPDSDRGSIDARAALLNDYSISMPSSERLLGMTRLREMAPNVSIPTEAAL
                     RLSTEVVEAGLPPGDAVGFRPTAVPDVWALASHDRRVVGLYRTGRLEAMMHDALHEVA
                     PEGITFIAFPPGIRADAEAIAAGNSLPGWQLSFFPIDTRIVDAAARGRKTFFIGVGAA
                     GIAIVALLGLGAGSVFRRQLHLARLKTDMVAAVSHELRTPVASMGVLVDGLLSDERLD
                     PVKTREYLALIAAENARLSRLIENFLTFSRLEHHKHQFVFAPVAPAAIAAAAMGAIRD
                     RMPSACDLRQDVAPDLPMVRADSDALGTALLNLLDNALKYTPAEKRIGVRVFRDNGMV
                     VFAVEDNGIGIPVREHRRIFRRYYRVDPRLSGETTGVGLGLSIVEFIVRAHGGTVSVQ
                     SESGAGSTFMMRVPCEGATVRREAPGAAA"
BASE COUNT          964 a         2108 c         2148 g         1004 t
ORIGIN      
        1 tcgtcacgac gctcgcgctc ctcatcggat ggggggcgat caccgtgagc cgcgccgggg
       61 tcggcatccc gctcactccg ccggcggacg cggccgccgt gtcgccggct gcggcgcgca
      121 cgtggcagga catcgtgctg gacacgttcc ccgagaacat cggccgggcg gtcgtcgagg
      181 ccgagatgct ccagatcgtg gtcttcagcg tgctcttcgg catcgcgctg gcgctgctgc
      241 ctgaagacaa gcggcggccg atgatggcct tcgccgaaag cctcgccgag acgatgttct
      301 cgttcaccaa gatcgtgatg ctgatggcgc ccatcggcgt cggtgcggcc atcgcctaca
      361 cggtcgggca gctcgggctg ggcgtgctct cgaacctgct gcagcttctg gccacgttct
      421 acgtcgccgt cggcctgttc gtcgtcggcg tcctgctccc cattgcgctg atcgcgaagg
      481 tgccgattcg ccgcttcgcc gaggccgtgg ccgagccggt gtcggtcgcc ttcgcgacga
      541 cgagctcgga agccgcgctg cctcgggcga tggaggcgat ggagcgcttc ggcgtgcctc
      601 gcccgatcgt ggctttcgtc atgccgaccg gttacagctt caacctggac ggcagcacgt
      661 tgtacctctc gctggccgcg atcttcgtcg cccaggctgc cggcatcgac ctgagcgtcg
      721 gccagcagtt cgtgatcctc ctgacgctga tgctctcgag caaaggagtc gcgggcgtgc
      781 cgcgcggcgc gctcgtcatc ctgctcggca cggtgtcgtc cttcggcctg cccgccgagc
      841 cggtgttcgt gttgctcggg atcgacgccg tgctggacat gctgcgcacg tcggtgaacg
      901 tgctcggcaa ctgcctagcg acggtggtca tcgcgaagtg ggaaggggag ccattgaaga
      961 cgtccggctg aagccggaca cggcgtacta gggtcgtcgg tttcgtcgcg cttccggcca
     1021 caaggccaac gcatcgggaa gcacgcggcg cgtggcgcgc aggcacgaca ggattctctc
     1081 gagccgtcgc tcatcgcggc ccgcccacgc aaggccgtac tgctgccgga tgccggaagg
     1141 caacagcccg atggtgacga cgcgattgac ccaggtggcc ggggccacgg cccatccacc
     1201 cgcgggggag agcacggcgc gtgacaactc gcgcccgtgg ctgccgacgg ccagcgcacc
     1261 cgacgcgagc atgcgttcca tgacgctccg cacgtcgtcc caccttcgcg gcacatcctg
     1321 cgggcgcgcc gcgagcgcga ccgcgatcgg gaacgcgtcc tcgcagtacg cgtcgcgttc
     1381 ctccgccgaa aggggcgcga ccaggcgttc gtaggccagg atcaccgact cgatcagcgt
     1441 ggcgtgaacc cacacgacga gtgccggatc ttcagccgaa tagcgagtcc cagccgggta
     1501 cgggccgacc ccgtcctcga gcacgccgtt gacgcggcgg tgtatcgccc ggatggcgtc
     1561 gagtgtgcgt tcgcggtcgg cgtcgcttcc aaacgtcagc gccagcatgg cgtgaactgt
     1621 gtggtacagc cgcgaggccg cggcccacgg cgtggcgcgg aagctgctgt gtccgaagac
     1681 gcccgcggcg acgagcggat gcgcgagctg caggaggatg gcgcgccccc agcccaggag
     1741 cacaatgcgt tcggcattga cgcgccacga caccgacgct gttcccgcca gatgattccg
     1801 tgcgaccgcc gacgcggccg tcgtctcttg gagggaacct gtcagggccg aaggcgcctt
     1861 ggcgttcacg attgcggttg tccctgcgcg tcctgattct ggggctggcc ggcaggatgg
     1921 tttcgaccgc catgacgaag cgcccacccg gcgatgccga agagcgccag cccgacggcg
     1981 ccgcggcgga cggtacgcca cacgccgacg cgcgggcgct cggcggggtg cagcattgca
     2041 cggtacgcgc cgaccgtctc cggcgcgtca acgtccgcgg gacgcgaagc gttgttcggg
     2101 cgctcgggca ccggaaccca tctggtgaga tacgcgaacg tctcggacgc acacagtgga
     2161 cattgctgag cgtcgtgaac ctcgtcgcaa tccaggcaga ggcgggccgt tcgcaattgc
     2221 atacgtccca gtgtaccgct agagctccag acgcccggcg acgaacgccg atcgtgccgt
     2281 gaaccccatc cgtgcataca gggcctgcgc ggcggtgttg cgatcgccga cgagcagcgt
     2341 gacttgccgc gccccgcgcg cagagccgac gcgaaagatc tcctcgacga gccgtcgtgc
     2401 cagcccctcg cgccgccggg atggacgcac tgcgacctgc gccaggtgca gcgtgccggg
     2461 cgagatccgc gtgacgagga ccagcgcgtc gaggcctctg tcgccgcgga ccacgcgcgt
     2521 gatcgccggc tccatcacgc cgcatcccgg gcgttccacg agactgcgga cgtagtgctc
     2581 ccattcctcg ggcgttccgt gcggcgcgaa atggcggccg tcgctcccgt aggactcgcg
     2641 aagcagctcg gcggccggcg tgagatcgcc atcgctccag gcgccggact ccgccgcgcc
     2701 ttcatcggcc gtgatggggc ggctcaagta gtgatagcgt tcgcacgcga atccacggtc
     2761 gcgaagcgcc atctcgaagt gttcggcgcg ggagggcagg aagcacgaca cgcgcgccgg
     2821 cctcgacgga cgcagcgcgg cggcgaggca ctcatcgagc agcgcgccgg tggcctccgg
     2881 catttcggcc accaccgcgc ccaggtgcgc ggtctcgccc tcgcgaagga agtaggacca
     2941 gccgcgaacc gtccgttcca cgtccctcgc gatgaaccct ggcagatccc ccgcgacgcg
     3001 cgcctgttcg atctcgcgcc aggcagacga cgcgtcccag gccagctcgc gtacccagta
     3061 ttcctgttcg cggtcgtaga gaacctgggt atccgcgggg tcggcttctc gccagtctcg
     3121 aacgatcacc tcggggggca cgaggtcagt gtacaagcac gcagggggca gggggcaggg
     3181 tgtgaggtgc gagggtgcga acgattagtt cgcgctcgcc accttctgtg ccggcgcgtc
     3241 gcccgagagc tcccgctgaa tgcgattctt ggtctcgacc gcggccgggc ttgcaaacga
     3301 cagatcgcgt ccgttctggg actggctcac gctcccgatt ttctcgaact cacgacggaa
     3361 ggatgccgag tctgtcaccg tcggcgcgtc cgcgacgacg atcgcgtgct gcatgtcgtg
     3421 tccgagcgcg gcgagctgtt tcgccttcga ttgcccgttg ggcacgtgca cgacgagata
     3481 gcggtagccg gcgttggccg tgaggaacgt caggcggccg gtccccgtct tcgaggtgac
     3541 gtccatccgc acatagagga tcacatccga cttgttcagc cgctctacga gcgctttcag
     3601 cgtcggtgac ctcttgacgc cctccgccag cagagcgacg agctcggcgt ccgacgcgcg
     3661 tacgttgccg tcccaggcat gcgctccggt gccgtcgaag gtggaaacgt tcggcctggc
     3721 gtcgccgact tgcgcccacg cgggcgtgga cgcacaaatc gccgccgcca cggccaggga
     3781 catggacagt cgtcgctgca tgaactttca gctcccctga tgactgtttc ggctcgtgta
     3841 acggagctcg acagtaggtt gtcccaggcc gggtgaagac tgtgattcct cagtgtcaat
     3901 ctgggaagcc tgtgtagaca ctcggtctac gagggcaggg ggcagggggc aagggggcag
     3961 ggggcaccct cgagtccttt gaaatcatca atccgcactc gcggctcagg atcaggagcg
     4021 gggatacggt ctacgccttc gaatgggcgg cgacgcgcgg catcgagcgg ctgggtgttg
     4081 gtcaggacgc gttcaaggct ggcgagccgt cgggctcgaa ccggtagccg atctcccgga
     4141 tcgtctggat gtagatcggg tggtgcggat tgggctcgat cttcgcgcga agcgtggcga
     4201 cgcagcggtc gatgctgcgc ggcgtcacga agaccgagct tccccacacg gcgtcgagga
     4261 tcgcgtcccg ggagagcgcg cagcccggtc gtgagacgaa gtaggcaaga agcctgaact
     4321 ccttggcggt cagatcgagc gccgcgccgt tgctcagtac cttgcggccc ttgaggtcga
     4381 cctgcacctg cccgaagcgg aacacgtcgc gttcgggcgc ccgccgcctg agaaacgcgc
     4441 ggattcgcgc gatcagttct ccgatccgga acggcttggt gacgtagtcg tcggcgccga
     4501 ggttcaggcc gagaacgacg tcctgctcct gccccttggc cgtgagcatc aggatgggca
     4561 cgtccaggcc tcgcgtgcgc acggcccggc aaatctcgta gccgttgacc ttcggcagca
     4621 tcacgtccag caggatcaga tcggcaaccc cctcgagcgc acgatcgaga cctatttgcc
     4681 cgtcgtgtgc cacccgcacc tcgaacccct cggcggccag cgcatccgag acgccgcgcg
     4741 tcagcgcggg ctcgtcctcg atcatcagaa tgcgcggcgc gctcatgcgg cggcgccggg
     4801 tgcctcgcgt cgcactgtcg caccctcgca cggaacccgc atcatgaacg tgctgcccgc
     4861 gcccgattcg ctctggacgc tgacggtccc tccgtgcgcg cgcacgatga attcgacgat
     4921 gctcaagccg aggccgacgc cggtggtctc gcccgacagc ctcgggtcca cgcggtagta
     4981 gcggcggaag atccggcgat gctcgcgcac cgggatcccg atgccgttgt cctcgacggc
     5041 gaacacaacc attccgttgt cccgaaacac gcgcacgccg atccgcttct cggcgggcgt
     5101 gtacttcagc gcgttgtcca gcaggttgag cagcgccgtg cccagcgcat cgctgtcggc
     5161 gcggaccatc ggcaggtctg gtgccacgtc ttgacgcagg tcgcacgccg aaggcatgcg
     5221 atcgcggatc gcgcccatgg cggcggcggc gatcgccgcc ggggccactg gggcgaagac
     5281 gaactgatgc ttgtggtgct cgaggcgcga gaaggtcagg aagttttcga tcagccggct
     5341 cagcctcgcg ttctcggcgg cgatgagcgc gaggtactcg cgggtcttga ccggatcgag
     5401 ccgttcgtcc gacaacaggc cgtccacgag cacacccatc gaggcgactg gcgttctgag
     5461 ctcgtgcgag acggctgcca ccatatccgt tttcagacgg gccaggtgca gctgccgcct
     5521 gaagacgctg ccggcgccga gaccgagcag ggcgacgatc gcgatgcccg ccgcgccgac
     5581 gccgatgaag aacgtcttcc gcccccgtgc cgcggcgtcg acgatccgcg tgtcgatcgg
     5641 gaagaacgag agctgccatc ccggcaggct gttgccggcc gcgatcgctt cggcatcggc
     5701 gcggatgccg ggcggaaacg cgatgaaggt gatcccttca ggcgcgacct cgtgcagcgc
     5761 gtcgtgcatc atcgcctcga gccggcccgt ccggtagagg ccgacgaccc gtcggtcgtg
     5821 cgacgcgagc gcccacacgt cggggacggc cgtcggccgg aatccgacag cgtcgcctgg
     5881 cggaagcccg gcctcgacca cttccgtact caggcgcagc gccgcttcgg tcgggatgct
     5941 cacgttcggc gccatctccc gcagccgcgt catgccaagc aggcgctcgg acgagggcat
     6001 cgagatggag tagtcgttca gcaaggcggc gcgcgcgtcg atcgagccgc gatcggaatc
     6061 cgggattcgg gactcgggat tcgggattcg cgccgcggcg ggctggggat aggcgatccc
     6121 gccatcggag tcgagcatca cgacgccgtc gccgatctcg tcgagaacga gtcgggcgaa
     6181 gtcctgctcg ggggtgttcg tgtgagtcaa ccgcgtggcg gcga
//
LOCUS       DBNGSH010000274         3118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3757281, whole genome shotgun sequence.
ACCESSION   DBNGSH010000274 DBNGSH010000000
VERSION     DBNGSH010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3757281"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="AC16CY_06415"
     CDS             complement(<1..398)
                     /locus_tag="AC16CY_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3353451.1"
                     /translation="MLSKTLQDGLNDYGIGAKIRALRLKKKIGLVDLGKHTGLSPALL
                     SKIERGRLFPTLPTLLRIALVFGVGLEYFFAGAREKPLVAVTRKSQRVELPERPGARE
                     AAYRFGSLDYPATERRFNSYYAEFLPMAPE"
     gene            583..1077
                     /locus_tag="AC16CY_06420"
     CDS             583..1077
                     /locus_tag="AC16CY_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR3353452.1"
                     /translation="MKTTFALTSLALVGIMAAASPARAQQTKDIVDTAVAAGSFTTLA
                     KALTAADLVATLKGPGPFTVFAPTDEAFAKLPAGTLDTLLKPENRAMLRRVLTYHVVP
                     GKVMAADVVKVNSAKAVSGDMLSITARGGTVMVDGAHVVKTDIAASNGVIHVVDAVLL
                     PREE"
     gene            1091..2272
                     /locus_tag="AC16CY_06425"
     CDS             1091..2272
                     /locus_tag="AC16CY_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR3353453.1"
                     /translation="MAATMLAGVRDYIGGRLLRVLEEGGRAVRWLARLPKRVGPGPAA
                     TTQVVAAPLSTFTYCSTIGAPTAEVFGWHERPGALAAMTPPGLLRIEEQEGGIRDGGR
                     VTLSIGVSPARVRWIVRHYGYIPGRRFCDEQVRGPFAVWRHAHLFEPIGASHTLHRDQ
                     LEFAVSRHSALNRVAAVLLRPVLTLAFAYRHRVVRASVGRPRRPGTARGATAAVAALA
                     AAATLQTATARAQTPEPVRTVPFVDLDRYAGDWFEIARFPNRFQRQCVGDVRASYARR
                     PDGRLDVVNRCRTADGQTEARGVARVVDERAFAKLKVRFAPAWLSLLPVVWGDYWIIG
                     LAEDYSWAVVGSPDRDYLWVLGRTPRLDERNAAAARAVARANGYDVERLAQTSHSEAD
                     R"
     gene            2340..2843
                     /locus_tag="AC16CY_06430"
     CDS             2340..2843
                     /locus_tag="AC16CY_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353454.1"
                     /translation="MTRKVIVNDRMQKGYVYFRTEPVGRNFASGFTPELTPKQMLALG
                     VFGGKYMTDCRDEFPASWIAHAKLCPDRHDPRLNYFGVNASQSLAEWRRKGWIHPQDP
                     RGWFQWYCRYYMGRRTRDDKRQIRRWRAIARHVAAIRKHCEKGDLDCRRRQRQAVLHW
                     AYDSRAI"
     gene            2833..>3118
                     /locus_tag="AC16CY_06435"
     CDS             2833..>3118
                     /locus_tag="AC16CY_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HIR3353455.1"
                     /translation="MPSNAADDSRPRPVRIGISACLLGQSVRFDGGHKRDALLTETFG
                     RFVEWVPVCPEVECGLGTPREAMRLVRAAHGVRLLTIKTGLDLTASMERFS"
BASE COUNT          502 a         1007 c         1069 g          540 t
ORIGIN      
        1 ttctcgggcg ccatcggcag gaactcggcg taatagctgt tgaaacggcg ctcggtcgcc
       61 gggtaatcga gcgacccgaa ccgatacgcc gcctctcgcg cgccagggcg ctccggcaat
      121 tccacgcgct ggctctttct cgtgacggcg acgagcggct tttcacgcgc gccagcgaag
      181 aagtactcca ggccgacacc aaagacgagc gcgatgcgca gcagcgtcgg cagcgtgggg
      241 aacagacgtc cgcgctcgat tttcgagagc agcgccggcg acagcccggt gtgcttgcca
      301 aggtcgacga ggccaatctt cttcttcagg cggagcgcac gaatcttggc gccaattccg
      361 tagtcgttca gtccgtcctg cagggtcttc gacagcatgg ctaacctcgt ttgactctgg
      421 ttcacatttc gcgtcgtttg tgtcgtcacg ctcacatgac ttcgcgtgcg atgcacagtt
      481 tcacttgtat gcaatttaac tttcattata gttaaagacg acagagtcaa tcacgaaagt
      541 cgtggcgtag ttcagttaaa gggattcgtt caaggagtgc acatgaagac cacgtttgcg
      601 ttgacctcac tagccctggt cgggatcatg gcggcggcct cgccggcgcg cgcgcagcag
      661 accaaggaca tcgtcgacac ggcggttgcc gccgggtcgt tcaccacgct ggccaaggcg
      721 ctcacggcgg ctgacctcgt tgccacgctc aaagggccgg gcccgttcac ggtctttgcg
      781 ccgaccgacg aggcgttcgc gaaactgcct gcgggcacgc tcgataccct gctgaagccg
      841 gaaaacaggg cgatgctgcg tcgcgtgctc acgtatcacg tcgtgcccgg caaggtgatg
      901 gcggccgatg tcgtcaaagt gaattccgcg aaggcggtca gcggcgacat gctgtcgatc
      961 acggcgcgcg ggggcaccgt gatggtggac ggagcgcacg tcgtcaagac cgacatcgcc
     1021 gcgagcaatg gcgttattca cgtcgttgac gccgtgctgc ttccgcggga ggaataggac
     1081 gctcgagagc atggccgcga cgatgcttgc cggcgtcagg gattacatcg gcggccgcct
     1141 ccttcgagtg ctcgaggaag gcggccgcgc ggtgcggtgg ctcgcgcggc tgcccaagcg
     1201 cgtcggtccg gggccggcgg cgaccaccca ggtcgtcgcg gcgcccctgt cgacgttcac
     1261 gtattgctcg acgatcggcg cgccgaccgc cgaggtgttc ggctggcacg agcggcccgg
     1321 agcgctcgcc gcgatgactc cgccggggct gctgcggatc gaggagcagg aaggtggcat
     1381 ccgcgatggg gggcgcgtca cgctgtcgat tggcgtcagc ccggcacgcg tccgttggat
     1441 cgtgcggcac tacggctaca ttcccggccg gcgattctgc gacgagcagg tgcgcggtcc
     1501 gttcgctgtc tggcgccacg cgcatctgtt cgagccgatc ggggcgtcgc acacgctcca
     1561 ccgggaccaa ctcgagttcg cggtctcccg ccatagcgcg ttgaaccgtg tcgcggcagt
     1621 cctgctccgg cccgtgctca cgctcgcgtt tgcgtaccgc catcgagtcg ttcgggccag
     1681 cgtcggccgt ccgcgccgac cgggcacggc ccgaggggcg acggcggcgg tggccgctct
     1741 cgccgctgcg gcgacgctgc aaacggcgac cgcgcgcgcg cagacgccgg aaccggttcg
     1801 gaccgtcccg ttcgtcgatc tggatcgata tgcgggcgac tggttcgaga tcgcgcggtt
     1861 tccgaatcgg tttcagcgcc agtgcgtcgg cgacgtgcgg gccagttacg cccgccgccc
     1921 tgacgggcgc ctcgacgtgg tcaaccggtg ccggaccgcc gatggtcaga ccgaggcgcg
     1981 cggcgtcgca cgcgtcgtcg acgagagagc cttcgcgaaa ctgaaggtgc gtttcgctcc
     2041 cgcgtggctc tcgctcctgc cggtcgtctg gggagactac tggatcatcg gcctcgcgga
     2101 ggattattcg tgggcggtgg tcggatcgcc ggaccgcgac tatctgtggg ttctcgggcg
     2161 cacgccgcga ctcgatgagc ggaatgccgc cgcggcgcgg gccgtcgccc gcgcgaacgg
     2221 atacgacgtc gagcgcctcg cgcagacgtc acactcggaa gcagatcgat gatgatccgg
     2281 aacgctgcgc gctgcggcat gaatgacgca accacggcct ggcgggcctt caccatctca
     2341 tgacacgaaa ggtcatcgtc aacgacagga tgcagaaggg ctacgtatac ttccggaccg
     2401 agcccgtggg acgcaacttc gcgtccggat tcacaccgga gctcacaccg aagcagatgc
     2461 tggcgctcgg cgtgttcggc ggcaaataca tgaccgactg ccgcgacgag tttcccgcga
     2521 gctggatcgc ccacgcgaag ctgtgtcccg accgtcatga tccgcgcctc aactacttcg
     2581 gcgtgaacgc ctcgcagtcg ctggccgagt ggcgccggaa agggtggatt catccgcagg
     2641 atccgcgcgg atggtttcag tggtactgcc gttactacat gggccggagg acgcgcgacg
     2701 acaagcggca gattcggcgc tggcgagcta tcgcgcggca cgtggcggcc atccggaagc
     2761 actgcgagaa aggcgacctc gactgccggc ggcggcagcg tcaggcggtg ctgcactggg
     2821 catatgacag ccgtgccatc taacgccgcg gacgattcga ggccgcggcc ggttcgcatc
     2881 ggcatctccg catgcctgct ggggcagtcg gtgcgcttcg acggcggcca caagcgcgac
     2941 gcgttgctga ccgagacgtt cggccgcttc gtcgagtggg tgccggtctg tccggaagtc
     3001 gagtgcggcc tcggcacgcc gcgcgaggcc atgcggctcg tgcgcgccgc gcacggcgtg
     3061 cggctgctga ccatcaagac cggcctcgat ctgacggctt cgatggagcg cttttctc
//
LOCUS       DBNGSH010000275         1859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_191376, whole genome shotgun sequence.
ACCESSION   DBNGSH010000275 DBNGSH010000000
VERSION     DBNGSH010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_191376"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="AC16CY_06440"
     CDS             complement(<1..652)
                     /locus_tag="AC16CY_06440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3353456.1"
                     /translation="MISRMKNVSRLAMLAAVTLALALAASRIVVAQSGDASVVSLIEP
                     IRKDHNLPALGAALITSQGLVSSGVAGVRKAKTDVAATVDDHWHLGSDTKAMTAVVLA
                     ILVERGTLTWDTSLGQVFPELASTFPANFRAITITQLLSHYAGLPPNLTWRAIARSSA
                     SVPEQRLNALKEAAKSTLSSNPGTRYEYSNLGYTIAGAIAERAAGQPWESLIREMVF"
     gene            713..1060
                     /locus_tag="AC16CY_06445"
     CDS             713..1060
                     /locus_tag="AC16CY_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR3353457.1"
                     /translation="MKIVIYQKPTCTTCRQVHAALVESGVDFDAVDYYVDPIPKKKLL
                     ELLRKMRMTPRELLRTKEPVYRTLRLAERDLTDEAMVDLMVKNPDLIQRPIVEKGSRA
                     ILARPAERLKDIL"
     gene            complement(1065..>1859)
                     /locus_tag="AC16CY_06450"
     CDS             complement(1065..>1859)
                     /locus_tag="AC16CY_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR3353458.1"
                     /translation="SYAQLGRDVFGGLGVLRGEQGDPAELPPEQWPDRDVAGCHMLYT
                     SVRRESNGSGWRTDYPWGQRNLLIRLSELTKTRVSWQSKGVPHVWLVALTDPALFECP
                     YLMASDVGTIGLSEEEMEALRIYLLKGGFLWVDDFWGEAAWDQWSRELARAMPPTEYT
                     IEDVPLSDPIFSSMMEVKKIPQVPGIGFWRAFGGQTTSERGAESAVPHFRAIRDRHGR
                     IIVVMTHNTDVSNSWERETEDPEYFYQFSIAGYALGIDVLLYAMTH"
BASE COUNT          330 a          615 c          588 g          326 t
ORIGIN      
        1 tgaacaccat ctcgcgaatc aacgattccc acggttgtcc agcggcccgc tcggcgatcg
       61 ctccagcgat cgtgtagccg agattcgaat actcgtacct cgttcccgga ttcgatgaca
      121 gcgtcgactt cgccgcctcc ttcagtgcgt tgaggcgctg ttccggcacc gaggcgctcg
      181 aacgcgcgat cgcccgccac gtgagattgg gcggtagtcc cgcgtagtgc gagagcaact
      241 gtgtgatggt gatcgcgcgg aagttcgcgg ggaatgtgga cgcgagctcc ggaaacacct
      301 ggccaagact cgtgtcccac gtgagcgtgc cgcgctcgac cagtatcgca aggacgacgg
      361 ccgtcatcgc tttcgtgtcc gagccaaggt gccagtgatc gtcgacggtc gcggcgacgt
      421 ccgtcttcgc cttcctcacg ccggccacgc cgctgctgac gagcccctgg ctcgtaatca
      481 gcgccgcgcc aagggcgggc aggttgtggt ccttgcgaat gggttcaatc aggctgacga
      541 ccgaggcgtc gcccgattgt gcgacgacga tccggcttgc ggccagcgcg agggcgagcg
      601 tcacggcggc gagcatggca aggcgtgaga cgttcttcat gcgcgaaatc atgatgcgtc
      661 ccagcgcgtg ctacgatccg cgccattgtc aagcacgatc tcttccgaac ccatgaagat
      721 cgtcatctac cagaagccga cctgcacgac gtgccggcag gtgcacgcgg ccctcgtcga
      781 gagcggtgtg gacttcgacg ccgtcgacta ctacgtcgat ccgattccga agaagaagct
      841 gctcgagctg cttcggaaga tgcgcatgac gccgcgcgag ctgctgcgaa cgaaggagcc
      901 tgtctacagg actctgcgcc tggccgagcg cgatctgacg gacgaggcga tggtggatct
      961 gatggtgaag aacccggatc tcattcaacg gccgatcgtc gagaaggggt cgcgcgcaat
     1021 ccttgcgcgt cctgccgagc gcctgaaaga catcctgtag cgcctcagtg cgtcatcgcg
     1081 tagagcagga cgtcgatccc cagcgcatag cccgcgatcg aaaactggta gaagtattcg
     1141 ggatcttccg tctcccgctc ccacgagttc gacacgtcgg tgttgtgcgt catcacgacg
     1201 ataatccgcc cgtgtctgtc gcgaatcgcg cggaagtgcg gcaccgccga ctccgcgccg
     1261 cgctcggacg tcgtctgacc gccaaacgcg cgccagaagc cgatgccggg gacctgcgga
     1321 atcttcttca cctccatcat cgagctgaag atcggatcgc tcagcggcac gtcttcgatc
     1381 gtgtactcgg ttggcggcat ggcccgggcg agctcgcgcg accactggtc ccaggccgct
     1441 tcaccccaga agtcatcgac ccacaggaag ccgcccttca gcaggtagat cctcagggcc
     1501 tccatttcct cctccgacag cccgatggtg ccgacgtcgg aggccatgag atacgggcat
     1561 tcgaagagcg cggggtccgt cagcgcgacg agccacacgt gcggaacgcc tttcgactgc
     1621 cagctgacgc gcgtcttcgt cagctccgac agacggatca ggaggttgcg ctgcccccag
     1681 ggatagtcgg tgcgccaccc ggagccgttg gattcgcgcc ggacgctcgt gtagagcatg
     1741 tggcatcccg ccacgtcccg gtcaggccac tgctcgggcg gcagttccgc cgggtcgccc
     1801 tgttcgccgc gcagcacgcc caggccgccg aagacgtccc gcccgagctg cgcgtacga
//
LOCUS       DBNGSH010000276         3829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1889413, whole genome shotgun sequence.
ACCESSION   DBNGSH010000276 DBNGSH010000000
VERSION     DBNGSH010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1889413"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1201
                     /locus_tag="AC16CY_06455"
                     /pseudo
     CDS             77..1201
                     /locus_tag="AC16CY_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            1198..3564
                     /locus_tag="AC16CY_06460"
     CDS             1198..3564
                     /locus_tag="AC16CY_06460"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3353459.1"
                     /translation="MNQPITLIKKSDGAVLKDVVCGMRVSPDSPHQIDYKGTRYAFCS
                     AGCLKKFQQDPERYLSPGAPPPQGMHHHEPPRVDVAPARPGGKWTCPMHPEIVRDGPG
                     SCPICGMALEPMTVSVEEPENVELVDMTRRFKVSTALTVPLVLIAMRDLIPGQPLDQA
                     LTPTRLAWVELLLATPVVVWGGWPFFVRGWESLVNRNLNMFTLIAMGVGVAYDYSVIA
                     TLVPGIFPASFRGHHGTVDVYFEAAAVIVTLVLLGQVLELRARSRTGAAIRALLGLAP
                     KTARRVRDGQEEDVPLEHVAPGDRLRVRPGEKVPVDGVVLEGSSAVDESMVTGEAIPV
                     AKGPGDRVVGATINGTGSFVMRAERVGADTLLARIVQMVADAQRSRAPIQKLADTVSG
                     YFVPAVVAVAAVTFTAWALVGPEPRAAFAIINSVAVLIIACPCALGLATPMSIMVAMG
                     KGATFGVLFRSAEAIEVMRKVDTLVVDKTGTLTVGKPKLVSVRAAEPWTEENLLRLAA
                     GLERGSEHPLAAAIVTGAAERGVGPAEAQVFQSMTGKGVVGRIDGHDVALGNRALMDD
                     LGVAPGAFADAAEGLRREGQTVMFVAIDARIAGLLGVADPIKETTPDAIRQLHAEGLR
                     IVMLTGDNRTTAEAVAKRLGIDDVIPEVLPDQKAETIKGLQRGGRVVAMAGDGINDAP
                     ALAQAHVGIAMGTGTDVAMESAGVTLVKGDLRGIVRARRLSRATIGNIKQNLFFAFVY
                     NALGVPLAAGVLYPFFGLLLSPMFAAAAMSFSSVSVVGNALRLRRLSL"
     gene            3616..>3829
                     /locus_tag="AC16CY_06465"
     CDS             3616..>3829
                     /locus_tag="AC16CY_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353460.1"
                     /translation="MRRSIASVAGSFIVAAVVAGQVAPPVQAPRFRVDPAWPKIPNNW
                     QFGQVASVSIDAQDHVWVLQRPGTLGP"
BASE COUNT          596 a         1245 c         1347 g          641 t
ORIGIN      
        1 gcagtttccg ggcttcccca acgtgtggac gcagccgatt cgcaaccggc tcgacatgct
       61 gctgaccggg atcaagacgc cggttggcat caaggtcctc ggcgccgact tgacggtaat
      121 cgaggggctc ggccgggaga tcgagcgcat tctccggcag gtgcccggca cgcgcagcgt
      181 ctatgccgag cgcgtcgcgc aggggtattt caccgacatc cgcatcgatc ggcaggcgat
      241 cgcgcgctac ggcctgacga tcgaggacgt ccaggacgtg attcagtccg ccctcggcgg
      301 cgagaacatc acgcagacga tcgaggggcg cgagcgctac ccggtcaacg tccgctatgc
      361 gcgcgagttc cgcgagagca tcccggcgct gcggcgtgtc ctcgtcaaga cgccgtcagg
      421 ggcggccgtc ccgctgggcc agctcgccga ggtctcgctg accaccggcc cggcgatgat
      481 ccgcgacgag gacgcgcagc tggccggcta cgtctacgtg gacaccgcca cgcgcgacgt
      541 cggcggatac gtggacgccg cgagggcggc catcgccggt gcgcttcgcc ttccacccgg
      601 atacacgctg cagtggaccg ggcaatacga gttccaggtc cgggcacgcg agcggctgca
      661 ggtcctgatt ccgctcgtct tcgtcatcat cttcatgctg ttgtacatga ccttccgctc
      721 cgcctcggag gcgaccatcg tgatgctgtc ggtcgtgtac gcgatgacgg gcggggtcat
      781 cctgcagtgg ctgctcgact acaacttctc cgtggccgtc tgggtggggt acatcgcgct
      841 gtacggcgtc gcggtgcaga ccggcgtcgt gatggtcgtc tacctgcacg aggcgctcga
      901 caagcggctg agcgccggag agatcacggc cgccgacgtt cttcaggcta ctatcgacgg
      961 ttctgttctt cggctgcggc cgaagctgat gacggtcacg gtcgtcatgg cgagcctgct
     1021 gccgatcatg tggagtacgg gcgtcgggtc cgacgtgatg aagccgattg ccgcaccgat
     1081 catcggtggc atgatcacgt cgacgatcca tgtgctcatc atcacgcccg tcatcttcta
     1141 catcatgaag atccgggcgc tgcgcaaggg acgcctgacg gtctcgggga tggcctcatg
     1201 aaccagccga tcacgttgat caagaagtcg gacggcgccg tcctcaagga cgtcgtgtgc
     1261 ggcatgcgcg tgtcgccgga cagcccgcac cagattgact acaagggcac gcgctacgcc
     1321 ttctgcagtg cggggtgcct gaagaagttc cagcaggatc cggaacgcta tctgtccccc
     1381 ggagcgccgc cgccccaggg gatgcaccac cacgagccgc cgcgagtgga cgtcgctccc
     1441 gcacgcccgg gcggcaagtg gacctgcccg atgcatccgg agatcgtgcg cgacggaccc
     1501 gggagctgcc cgatctgcgg catggcgctc gagccgatga cggtgagcgt ggaggaaccg
     1561 gagaacgtcg agctcgtcga catgacgcgg cggttcaagg tcagtacggc gttgaccgtg
     1621 ccgctcgtgc tgatcgcgat gcgcgatctg attcccggac agccgctcga ccaggcgctg
     1681 acaccgacgc ggcttgcgtg ggttgagctg ctgctggcga cgcccgtcgt ggtgtggggc
     1741 ggctggccgt tcttcgtccg cggctgggaa tcgctcgtca accgtaacct caacatgttc
     1801 acgttgatcg cgatgggcgt cggcgtggcc tacgactaca gcgtcatcgc gacgctcgtc
     1861 ccaggcatct ttcccgcgtc gttccgcggc catcacggca ccgtggacgt ttacttcgag
     1921 gccgccgccg tgatcgtcac gctcgtgctg ctcggccagg tcctcgagct cagagcacgc
     1981 agccggactg gcgccgccat ccgcgccctg ctggggcttg cgccgaagac cgcccgccgt
     2041 gtccgcgacg gacaggaaga ggacgtcccg ctcgagcacg tcgcgccagg cgatcgtctc
     2101 cgcgtgcggc caggggagaa ggtcccggtc gatggcgtgg tcctcgaagg ctcgagcgcc
     2161 gtggacgagt cgatggtcac cggtgaggcg atcccagtgg ccaagggacc aggcgaccgc
     2221 gtcgtcggcg cgaccatcaa cggcaccggc tcgttcgtca tgcgcgccga gcgcgtcggc
     2281 gccgacacgc tgctggcgcg catcgtccag atggtggcgg acgcgcagcg cagccgcgcg
     2341 ccaatccaga agctggcgga tacggtgtcc ggctacttcg tgccggcagt tgtcgccgtt
     2401 gcggccgtca ccttcaccgc gtgggcgctc gtgggccctg agccgcgggc ggcgttcgcc
     2461 atcatcaact ccgtcgccgt cctcatcatc gcgtgcccgt gcgctctcgg gttggcgacg
     2521 ccgatgtcga tcatggtggc gatggggaag ggcgctacct tcggcgtgct gttccggagc
     2581 gccgaggcga tcgaagtgat gcgaaaagtc gacacgctcg tcgtggacaa gacgggcacg
     2641 ttgacggtcg gcaagccgaa gctggtcagc gtgcgcgccg ccgagccatg gaccgaggag
     2701 aacctgctgc gactggcagc gggcctcgag cggggcagcg agcatccgct ggccgccgcc
     2761 atcgtcacgg gagccgctga gcgaggggtc gggcccgccg aggcgcaggt atttcagtcg
     2821 atgaccggca agggcgtcgt tggccgcatc gacgggcacg acgtcgcgct gggcaatcgt
     2881 gctctcatgg acgatctcgg cgtggccccg ggcgcttttg ccgatgcagc agagggcctt
     2941 cggcgcgagg ggcagacggt catgttcgtc gcgatcgacg ctcgtatcgc aggtcttctc
     3001 ggcgtggccg atccgatcaa agagacgacg cccgacgcga tccggcagct ccacgccgag
     3061 gggcttcgca tcgtgatgct gaccggagac aaccgcacga cggccgaggc cgtggcgaaa
     3121 cggcttggca tcgacgatgt gattccagag gtgctgccgg atcagaaagc ggagacgatc
     3181 aaggggctgc aacgtggagg ccgcgtcgtg gcgatggccg gcgacggtat caacgacgcg
     3241 cccgcgctcg cccaggccca cgtcgggatc gccatgggca ccgggaccga cgtcgcgatg
     3301 gagagcgcgg gggtgacgct ggtgaagggc gatctgcgcg gcatcgtccg cgcgcggcgc
     3361 ctgagccggg ccacgatcgg caacatcaag cagaatctgt tcttcgcgtt cgtctacaac
     3421 gcgctcggcg tgccgctcgc cgccggcgtg ctctatccgt tcttcggcct gctgctcagc
     3481 ccgatgttcg cggcggcggc gatgagtttc agctccgtgt cggtggtcgg gaacgcgctc
     3541 cggctgaggc gcctgagcct gtaacgtgga cgtgaataaa tgtgttgggg tctgacccct
     3601 acacaggagc ggatcatgag gagatcgatc gcatcggtgg ccggctcgtt catcgtcgcc
     3661 gccgtcgtcg caggccaggt cgcgccgccc gtccaggcgc cgaggttcag ggttgacccg
     3721 gcgtggccga agatcccgaa caactggcag ttcgggcagg tcgcgagcgt gtcgatcgat
     3781 gcgcaggatc acgtgtgggt gctgcagcgc ccgggcacgc tcggcccgg
//
LOCUS       DBNGSH010000277         3718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_22258, whole genome shotgun sequence.
ACCESSION   DBNGSH010000277 DBNGSH010000000
VERSION     DBNGSH010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_22258"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="AC16CY_06470"
     CDS             <1..489
                     /locus_tag="AC16CY_06470"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF012799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3353461.1"
                     /translation="FGRGAEETLALAFAGIPFEVVPGVSTAVAGPELAGIPVTHRGMA
                     SGFLVLAGQTGDGLNEALASVRPGSMTLVVMMGLAARDEIARRLVEHGWMARTPSAIV
                     CGVSTPREWVWTGTLEELAAQTPPEGVAGVIVVGEVVSIREVLAAGRVRPPADEVKYG
                     RR"
     gene            476..2305
                     /locus_tag="AC16CY_06475"
     CDS             476..2305
                     /locus_tag="AC16CY_06475"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR3353462.1"
                     /translation="MAVADDSKALGRARLDFAEEHDIDEFVEVLGKYERGEITPEAWR
                     RFRLVRGTYGQRQDNVQMLRIKIPQGILTASQMRALAGVATRYSRGFCHVTTRQNIQY
                     HFVQLDVVEEVMRELAAEGLTTREACGNSVRNITGCPYAGTSETEIFDVTPYAEALTR
                     YFLRHPLASVLPRKFKIAFEGCVEDHALASIHDIGWRARIVEGTRGFRVTIAGGTSIL
                     PVSGYVLYEFLPVGEMFNVAEAVLRVFHRFGDYKHPQRNRMKFTVRSLGWEQFRARVE
                     ECLEEFRHEGGACLPFDPDAVRSEGAPDWTPAEPPTLQAVAAASKTPVTGPGILPGTV
                     RLQPLPDAFVRWMRSNVSKQRQAGYCHVTVRLPLGDFTAGQMRVLADLGEAYGDGTMR
                     LTVGQNVLFRWVKAASVEPFYHRLAAAGLGAPDAGTLADIVSCPGAESCRLAVTQSRG
                     LGRELTEHLNVRPDLVDLVASGNIKISGCPNGCGQHHIASIGFQGSVRKLGARAVPQY
                     FVLVGGGCTDEGVAHFGKVVSKVPVHRLSDAVDRLLLLYRDHRQGSEELGAFFRRVPA
                     SMATDALKDLATLLPNEATEQDFVDLAETQAFTPEVMDGECSA"
     gene            complement(2324..>3718)
                     /locus_tag="AC16CY_06480"
     CDS             complement(2324..>3718)
                     /locus_tag="AC16CY_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3353463.1"
                     /translation="QSGTGSLRALVYLDEVFGYLPPVANPPSKAPLLTLLKQARAYGV
                     GIVLATQNPVDLDYKALSNTGTWFLGKLQTERDKARVLDGLEGVAGSLDRQTLDKTLS
                     ALPGRAFLMHNVHEGAPVVFQTRWALSYLKGPMGREDLQRLAAPEETAVPSRPASPAA
                     HQPSPKPAVPAGVPEYVLAGSSETPTGFQPVLYGVSRVHYTDTRRGIDVARSVQATVP
                     FGHGAIPIDWDQADAAAPPPDALKEPKWMPEAARCEAPPPAALDPKRYVEWSRDFADW
                     IVRTQPLKLFSVPSLKLMSEPGETERDFRIRAQQAAREARDESVGRLRERYAPKVARL
                     TEKVARGRELVDKEQQQVQQQKLQTAVSFGATMLGALMGRKTVSVSTLGRATTAARGV
                     GRSMKEAQDVARARERLQEAEADLAAAEAELRDEIAALEHGPDAAAIDVIEIKPKRGG
                     VDVRLVALAWKGID"
BASE COUNT          549 a         1242 c         1285 g          642 t
ORIGIN      
        1 ttcggcaggg gggccgagga gacgctcgcc ctcgcgtttg cggggattcc cttcgaggtc
       61 gtccccggcg tcagcacggc tgttgccggc ccggagctgg ccggcattcc ggtcacgcac
      121 cgcggaatgg cgtccgggtt tctcgtgctg gccggccaga cgggagatgg cttgaacgag
      181 gcgctggcct cggtccgccc gggcagtatg acgctggtgg tgatgatggg gctcgcggcc
      241 cgcgacgaga tcgcccggcg gcttgttgaa cacgggtgga tggcccggac gccgtcggcc
      301 attgtgtgcg gtgtctcgac gccgcgagaa tgggtgtgga cgggtacgct ggaagaactg
      361 gcagcccaga cgccgcccga gggcgtggcc ggtgtgatcg tggtcgggga agtggtatcc
      421 attcgtgagg tgctggctgc gggacgcgta cgtcccccgg ccgatgaggt gaagtatggc
      481 cgtcgctgac gactcgaagg cgcttggccg tgcgcgtctc gatttcgccg aggagcacga
      541 catcgacgag ttcgtcgagg tgctggggaa atacgagcgc ggcgagatca ccccggaagc
      601 atggcggcgt ttcaggctcg tgcgcggcac ctacgggcag cgtcaggaca acgtgcagat
      661 gctgcggatc aagatcccgc aggggattct gaccgcgtcg cagatgcgtg cgctggccgg
      721 cgtcgcgacc cgctattccc gcggcttctg ccacgtcacc acgcggcaga acatccagta
      781 ccacttcgtc cagctcgacg tcgtcgaaga ggtgatgcgc gagctcgccg ccgagggcct
      841 cacgacgcgc gaggcctgcg ggaattcggt ccgcaacatc acgggctgcc cgtacgcggg
      901 aacgtccgag accgagatct tcgacgtcac gccgtacgct gaagcgttga cgcggtactt
      961 cctccggcat ccgctcgcgt ctgtcctgcc gagaaagttc aagatcgcct tcgaggggtg
     1021 cgtcgaggac cacgccctcg cctcgattca cgacatcggc tggcgcgccc gcatcgtgga
     1081 ggggacgcgc ggattccgcg tcaccatcgc cggcggcacg tccatcctgc cggtgtccgg
     1141 atacgtgctc tacgagttcc tgccggtcgg cgagatgttc aacgtcgccg aggccgtgct
     1201 gcgcgtgttc caccggtttg gcgactacaa gcacccccag cgcaaccgca tgaagttcac
     1261 ggtgaggtcg ctcggctggg agcagttccg cgcgcgcgtc gaggagtgcc tggaggagtt
     1321 ccggcatgaa ggaggcgcgt gcctgccgtt cgatcctgac gccgtacgat ccgagggagc
     1381 gcccgactgg actcccgcgg agcctccgac gctgcaggcc gtggcggcgg cgtcaaagac
     1441 gcccgtgacc ggtccgggaa tcctccccgg tacggtgcgg ctgcagccgc tgcccgacgc
     1501 gttcgtccgc tggatgcgga gcaacgtcag caagcagcgg caggccggct actgccacgt
     1561 caccgtgcgg ctgcctctcg gcgacttcac cgcgggacag atgcgggtgc tcgcggatct
     1621 tggcgaagcc tacggcgacg gcacgatgag gttgaccgtc gggcagaacg tgctgttccg
     1681 ttgggtgaaa gccgcctcgg tcgagccttt ctaccatcgc ctggcggcgg ccggcctcgg
     1741 cgcgcccgac gccggaacgc ttgccgacat cgtcagctgc cccggcgccg aaagctgccg
     1801 tcttgccgtg acgcaatctc ggggcctggg gcgcgagctg accgagcact tgaacgttcg
     1861 tccggacctg gtggacctcg tggcgtcggg caacatcaag atcagcggct gcccgaacgg
     1921 gtgcggccag caccacatcg cgtcgatcgg gttccagggc agcgtgcgca agctcggcgc
     1981 ccgcgcggtg ccgcagtatt tcgtcctcgt cggcgggggg tgcacggacg aaggcgtggc
     2041 gcacttcgga aaagtcgtgt cgaaggtgcc cgtccaccgt ctgtcggacg ccgtcgatcg
     2101 actgctgctg ctgtaccgcg accatcggca gggcagcgaa gaactgggcg ccttcttccg
     2161 tcgtgtcccc gcgtcgatgg cgaccgacgc cctcaaggat ctcgcgacgc ttctgccgaa
     2221 cgaggcaacc gaacaggact tcgtcgatct cgccgagaca caggccttca cgccggaggt
     2281 catggacggc gagtgctctg cttagccggt agcccgtcgc acgctagtcg atccccttcc
     2341 aggcgagcgc gacgaggcgg acgtcaacgc cgccgcgctt cggcttgatt tcgatcacgt
     2401 cgattgccgc ggcatccggg ccgtgctcga gcgccgcgat ctcgtcccgt aattcggcct
     2461 cggcggccgc cagatccgcc tcggcctcct gcagtcgttc gcgcgcgcgc gcgacgtcct
     2521 gggcttcctt catcgagcgt ccgacgccgc gcgcggccgt ggttgcgcgt ccgagcgtcg
     2581 agacggagac cgttttcctg cccatgagcg cgccgagcat cgttgcgccg aaggatacgg
     2641 ccgtttgcag tttctgctgc tgaacctgct gttgttcctt gtccaccagc tcacggcccc
     2701 gcgcgacctt ctccgtcaag cgcgcgactt tcggggcata gcgctctctc agccgtccga
     2761 ctgattcgtc ccgggcctcc cgcgcggcct gctgagcgcg gatcctgaaa tcgcgctccg
     2821 tctctccggg ctcggacatc agcttgagtg agggaacgga gaacagtttc aagggctgcg
     2881 tgcgcacgat ccagtcggcg aagtcccggc tccactcgac gtatcgcttc ggatcgagcg
     2941 cggccggggg cggcgcttcg cagcgtgcag cctcgggcat ccacttcggt tccttgagag
     3001 cgtctggagg cggcgcggcg gcgtcggcct ggtcccagtc aatcgggatt gccccatggc
     3061 cgaacggcac cgtggcctgc acactcctcg cgacgtcgat gcctcgcctc gtgtcggtgt
     3121 aatggacgcg cgacacgccg tagagcacgg gctgaaaccc agtcggtgtc tcactcgacc
     3181 ccgcgagtac gtactcgggg acaccggcgg gcacggctgg cttgggcgac ggttggtgcg
     3241 ccgcaggcga tgccggccgc gaaggaaccg ccgtctcctc gggcgccgcc aggcgctgca
     3301 gatcttcgcg tcccatcgga cctttgaggt acgacaacgc ccatcgtgtc tggaacacga
     3361 ccggcgctcc ctcgtgaacg ttgtgcatga gaaacgcgcg tcccggcaag gctgacaggg
     3421 tcttgtcgag cgtctgccga tcgaggctgc cggccacgcc ttcgagcccg tcgagcacgc
     3481 gcgccttgtc gcgctcggtc tgcagctttc cgaggaacca cgtccccgtg ttcgacaacg
     3541 ccttgtagtc caggtccacg gggttctgcg tcgccagtac gatgccgacg ccgtaggcgc
     3601 gagcctgttt gaggagcgtg agaagcggcg ccttcgacgg cgggttggcc acgggcggca
     3661 ggtagccgaa cacttcgtcc aggtagacca gcgcccgcag cgatccggtc ccgctctg
//
LOCUS       DBNGSH010000278         4177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1210129, whole genome shotgun sequence.
ACCESSION   DBNGSH010000278 DBNGSH010000000
VERSION     DBNGSH010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1210129"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="AC16CY_06485"
     CDS             <1..1244
                     /locus_tag="AC16CY_06485"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3353464.1"
                     /translation="ANGLNVILHRDTTVPAVAINVWYYVGSANEKPGRTGFAHLFEHL
                     MFEGSKHVPEGQFDLLLEGAGGDNNGSTNNDRTNYVINVPSNALELALYLESDRMGYL
                     LDTMSPARVDGQRDVVKNERRQSYENRPYGMASIELDKMLWPAAHPYSWPTIGYMEDL
                     TAASHADVVEFFKRYYAPNNASLVIAGDIDFDRTRALVEKWFGEIPRGVAVEPIAPPS
                     AILTGVQKKTLTDKVSLPRLYLGWLTPRFYAPGDSALDVVSSVLAGGKNSRLYKRLVY
                     DMQIAQDVSAFQQSGAIGSSFLVVATARRGHSAAELQTAIDDELEKLRRESPNPREIQ
                     RAINQIEASFYRRMERIGGFGGKADQLNAYAFAGGSPDFFAEDLARYTSLSPDDIRSA
                     AARWLPAERRVELVVEPEVKK"
     gene            1241..2707
                     /locus_tag="AC16CY_06490"
     CDS             1241..2707
                     /locus_tag="AC16CY_06490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3353465.1"
                     /translation="MSAKGLGVVALGVGSVAALLLATSAQLGAQQAPDRSKPPALAAT
                     PELKLPAIQKRALSNGLAVWVVEAHEVPLVQVTLLIKAGSGDDPAGKFGVASLTAAML
                     DEGAGSRSALELADELEFLGADLGTGSSFDASAVRLNVPVSRLRAALPLMADVAMRPT
                     FPEAELGRLRQERLTALLQARDDAAQVAPMAFARLLFGPSHRYGTGAAGTGATLKSFT
                     TADLRAFHSAIFQPGNASIIVAGDVTADAALPQLEMHFGSWRSQAGAASRTPVPNAPQ
                     PPRGEIVIVDMPGAAQSQIRMGWVGVPRSTPDYFTLQVLNTVLGGSFTSRLNQNLREE
                     HGYSYGASSRFDMRLAAGPFQAGAGVQTDKTSESVREFFNELNRIGKPIGDDELTKAK
                     NYVGLSFPGEFETIGDLSAQLEQQIIYGLPDTYYAQYVKNIQSVTSAAAQKAAATYIQ
                     PTRFLVLVVGDRKIIEPGIRALNLGPVRARSVQEALGE"
     gene            2708..3040
                     /locus_tag="AC16CY_06495"
     CDS             2708..3040
                     /locus_tag="AC16CY_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HIR3353466.1"
                     /translation="METYYHPKDLARFGDVGKEAPELWQKFSEWYNAVFKDGALSERE
                     KALIALGVAHAVQCPYCIDAFSSASLEKGSTPEQMTEAVHVAAAIRGGASLVHGVQMR
                     HHVDKLSM"
     gene            3135..>4177
                     /gene="arsS"
                     /locus_tag="AC16CY_06500"
     CDS             3135..>4177
                     /gene="arsS"
                     /locus_tag="AC16CY_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671343.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HIR3353467.1"
                     /translation="MNVALPTLLNTHDPLADPAAQRAALDALPLASGFGEALARADRS
                     PLRADGVSVLQMNVGKRCNQTCRHCHVDAGPDRTEVMPPDIVDGCLRFLETSGIPTLD
                     ITGGAPELHPSFRDIVRRARAMGRHVMDRCNLTITRLPSYQDLPEFLAAHQVEVIASL
                     PSFAARQTDAQRGSGVFDDSIAALRRFNELGYGIEGSGLVLNLVTNPVGAFLPGGQRS
                     LEADWKRELHRKFGLVFNRLYTITNMPISRFLQYLIDSGNLQGYMDKLVASFNPAAVD
                     GLMCRSTLSVGWDGRLYDCDFNQMIELGTAPDVPQSIVNASLDALASRRIVIGHHCFG
                     CTAGAGSSCGGAIT"
BASE COUNT          681 a         1424 c         1395 g          677 t
ORIGIN      
        1 tcgccaatgg cttgaacgtc atccttcatc gcgacacgac ggttcccgcc gtcgcgatca
       61 acgtctggta ctacgtcggc tcggcgaacg agaagcccgg ccgcacgggg ttcgcgcacc
      121 tgttcgagca cctgatgttc gagggatcca agcacgtgcc cgagggccag ttcgacctcc
      181 tgctcgaggg cgccggcggc gacaacaacg gctcgaccaa caacgaccgg accaactacg
      241 tcatcaacgt cccgtccaac gcgctcgagc tcgcgctgta cctcgagtcg gatcggatgg
      301 gttacctgct cgatacgatg tcgcccgcgc gcgtggacgg ccagcgcgac gtcgtcaaga
      361 acgaacgccg gcagagctac gaaaaccgac cctacggcat ggcgtcgatc gagctggaca
      421 agatgctctg gcccgcggcg cacccgtaca gctggcctac gatcggctac atggaggacc
      481 tgacggcggc cagccacgcc gatgtcgtcg agttcttcaa gaggtactac gcgcccaaca
      541 acgcgagcct cgtcatcgcg ggcgacatcg acttcgatcg cacgcgagcg ctcgtcgaga
      601 aatggttcgg agagattccg cgcggcgtgg ccgtggagcc gattgccccg ccatccgcca
      661 tcctgaccgg cgtccagaag aagacgttga ccgacaaggt ctcgctgccc cgcctgtatc
      721 tcggctggct gacaccgcgc ttctatgcgc cgggggactc cgccctcgac gtcgtctcgt
      781 cggtcctggc cggcggcaag aactcccgtc tctacaagcg actcgtctac gacatgcaga
      841 tcgcccagga tgtgtccgcg ttccagcagt cgggcgcgat cggcagctcc ttcctcgtcg
      901 tcgccacggc gcgccgcgga cattcggcgg cggagctgca gacggcgatc gacgacgagc
      961 tcgagaagct gcggcgcgag agcccgaacc cgcgcgagat ccagcgcgcg atcaaccaga
     1021 tcgaggcgtc gttctaccgg cggatggagc gcatcggcgg cttcggcggc aaggccgatc
     1081 agctgaacgc gtacgccttc gccggcggca gcccggattt cttcgccgag gaccttgccc
     1141 gctacacctc gctcagtccc gacgacatcc ggtcggctgc cgcgcggtgg cttcccgccg
     1201 agcgcagggt ggagctcgtc gtcgagccgg aggtgaagaa atgagcgcca agggcttggg
     1261 agttgtggcg ttgggcgttg ggagtgtggc cgcgctgctt ctcgcgacgt ctgcgcagct
     1321 tggagcgcaa caggcgccgg atcggtcgaa gccgcccgcc ctcgcggcga ctccggaact
     1381 gaagctgccg gcgatacaga agcgtgcgct gtcgaatggc ctggcggtat gggtcgtcga
     1441 agcgcatgaa gtcccgctcg tgcaggtcac gctgctgatc aaggccggga gcggcgacga
     1501 cccggccgga aagttcggcg tggcaagctt gacggccgcg atgctcgacg agggcgccgg
     1561 gtcgcgatcg gctctcgagc tcgcggacga actggagttc ctgggcgccg atctcggcac
     1621 cggcagctcc ttcgatgcgt ccgcggtcag gctcaacgtg ccggtgtccc gcctccgcgc
     1681 ggccctgccc ctgatggccg acgtggcgat gcggccgacg tttcccgagg ccgagctcgg
     1741 gcgcctcagg caggagcgcc tgacggcgct cctccaggct cgggacgacg ccgcgcaggt
     1801 tgcgccgatg gcattcgcgc gcctgctgtt cggtccgtcg caccggtatg gcaccggcgc
     1861 cgccgggacg ggagcgaccc tcaagagctt cacgaccgcg gacctgcggg cgtttcattc
     1921 cgcgatattt cagccgggga acgcgtcgat catcgtcgcc ggcgacgtca cggccgatgc
     1981 cgcgctgccg cagctcgaga tgcatttcgg atcctggcgc agccaggcgg gcgcggcctc
     2041 ccggactccc gtcccgaacg cgccgcagcc gccgcggggc gagatcgtca tcgtggacat
     2101 gcccggggcc gcgcagtcgc agattcgcat ggggtgggtg ggcgttcccc ggtccacgcc
     2161 cgactatttc acgctgcagg tgctgaacac ggtgctcggc gggtcgttca cctcgcggct
     2221 caatcagaac ctccgggaag agcacgggta ctcgtatggc gcaagctcgc gcttcgacat
     2281 gcggctcgcg gccggtcctt tccaggccgg cgcgggcgtc cagaccgaca agacatccga
     2341 gtcggttcgc gagttcttca acgagctgaa ccggatcggc aaaccgatcg gcgacgacga
     2401 gctgacgaag gccaagaatt acgtcggcct cagctttcca ggcgagttcg agacgatcgg
     2461 cgatctctcc gcgcagctcg agcagcagat catctacggc ctgcccgaca cctactacgc
     2521 gcagtacgtg aagaacatcc agtcggtgac gtcggccgcc gcccagaagg ccgcggcgac
     2581 ctacatccag ccgacgcgct ttctcgtgct cgtcgtcggc gatcgcaaga tcatcgagcc
     2641 gggcatccgt gcgctcaatc tcggtcccgt gcgcgcgcgg tcggtacagg aagcattggg
     2701 ggaatgagtg gagacgtact accatccgaa ggacctcgcg cgattcggcg acgtcggcaa
     2761 ggaagctccg gagctgtggc agaagttcag cgagtggtac aacgccgtgt tcaaggacgg
     2821 ggccttgagc gagcgcgaga aggcgctcat cgcgctcggc gtcgcgcacg cggtgcaatg
     2881 tccctactgc atcgacgcct tttcgagcgc gtcgctcgag aagggatcga cgcctgagca
     2941 gatgaccgag gccgtgcacg tcgcggcggc gattcgcgga ggagcgtccc tcgtgcacgg
     3001 cgtgcagatg cgccaccacg tggacaagct gtcgatgtag cgcgcccggt tgggagttgg
     3061 cccgcctccg cgccgaaggc gcttctgcga gcctcgccgt agccttggcg aaggcgggaa
     3121 ttgggcgttg ggaattgaac gtggcgctac cgactctgct gaacacacat gaccccctgg
     3181 ccgacccggc cgcgcagcgc gccgcgctcg acgcgctgcc gctcgcctcc gggttcggag
     3241 aggcgctggc gcgagctgac cgctcgcctc tgcgcgccga cggagtttcg gtgctgcaga
     3301 tgaacgtcgg caagcgctgc aaccagacct gccgtcattg ccatgtggac gcggggcccg
     3361 accgtaccga ggtgatgccg cccgatatcg tggacggctg cctccgcttt ctcgagacca
     3421 gcggcattcc aacgctcgat atcacgggcg gcgcgccgga gcttcatccc tcgttccgcg
     3481 acatcgtgcg ccgcgcccga gccatgggcc gccacgtgat ggaccggtgc aacctgacga
     3541 tcacgcgcct gccgagctac caggacctgc ccgagttcct cgcggcacac caggtcgagg
     3601 tcatcgcctc gctcccgtcg tttgccgccc gccagaccga cgcgcagcgc gggtccggcg
     3661 tcttcgacga ttcgatcgcg gcgctgcgcc ggttcaacga gctcggctac gggatcgagg
     3721 gttccggcct cgtgctgaac ctggtgacca accccgtggg ggccttcctg ccgggcgggc
     3781 agcgctcgct cgaggccgac tggaagcgcg agctgcatcg caagttcggc ctcgtcttca
     3841 atcggctcta cacgatcacc aacatgccca tcagccgctt cctgcagtac ctgatcgatt
     3901 caggcaacct gcagggctac atggacaagc tggtcgcgtc gttcaatccg gcggcggtgg
     3961 acggcctgat gtgccgctcg acgctgtcgg tcggctggga cgggcggctc tacgattgcg
     4021 acttcaacca gatgatcgag ctgggaaccg cgccggacgt tccgcagtcg attgtcaacg
     4081 cctcgctcga cgcactcgcc tcgcgccgca tcgtgatcgg acaccactgc ttcgggtgca
     4141 cggccggcgc cggatcgagc tgcggcggcg ccatcac
//
LOCUS       DBNGSH010000279         1966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3248081, whole genome shotgun sequence.
ACCESSION   DBNGSH010000279 DBNGSH010000000
VERSION     DBNGSH010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3248081"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="AC16CY_06505"
     CDS             complement(<1..316)
                     /locus_tag="AC16CY_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353468.1"
                     /translation="MTGRVLVGAALVLLLPVSAAWAQGPRTPWGDPDIEGIWTNATLT
                     PLQRPADLASKEFFTPEEAAQFARVRIEQTNADRPLPPGQVGAYNDVFFERGKGTVKS
                     RRT"
     gene            complement(348..1517)
                     /locus_tag="AC16CY_06510"
     CDS             complement(348..1517)
                     /locus_tag="AC16CY_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HIR3353469.1"
                     /translation="MTSTRERLLLIAAFLSVSSPHLTAQNPVAPLFSSPEVGSDRRIT
                     LRIAAPQAQAVRLSASDIPGVGQGAEMTKGQNGVWETTVGPVPAGAYRYRFTVDGVAT
                     MDPRNPSTSESNTNAWSLVYVDGSDTSDTKNVPHGGVSEVTYHSTALNAVRRMHVYTP
                     PGYETSTRKYPVFYLLHGAGDSDDSWSTVGRAGFILDNLIAARKARPMIVVMPAGHIR
                     GLAAPLSATGPDGFVNDFVADVMPYVQKNYRASTDRASTAIAGLSMGGNQALNIAIPN
                     LDRFAYIGVYSSGLFGAFPQRGRGGPPAPPAAASGPTEWETRYAAKLADANVKKGLKL
                     FWFSTGKDDFLLGTTVATVDLFRKHGFTPIYQESAGGHTWLNWRDYLSEFAPQLF"
     gene            1636..>1966
                     /locus_tag="AC16CY_06515"
     CDS             1636..>1966
                     /locus_tag="AC16CY_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353470.1"
                     /translation="MNVKSYLISLPERLVRSIVGLGAGVAREVGEVAIPDAVRRTRLY
                     QNLVDTTLRFLIEQVGGVEGVFGDDAMLADDFLVRRTAGNAIEALGIVAFRASPVWVL
                     AALSDVCG"
BASE COUNT          308 a          628 c          675 g          355 t
ORIGIN      
        1 aggtgcgccg gctcttgacc gtgcccttgc ctcgctcgaa gaagacgtcg ttgtacgcgc
       61 cgacttgccc gggcggcagc ggacggtcgg cgttggtctg ctcgatgcgc acgcgggcga
      121 actgggcggc ttcctccggc gtgaagaact ccttcgaggc gagatcggcg ggacgctgga
      181 gcggcgtcag cgtggcgttg gtccagatgc cttcaatgtc ggggtcaccc cagggcgtcc
      241 gcggcccctg cgcccaagcc gccgataccg gcaacaggag gacgagggct gcaccgacga
      301 gaacacgccc ggtcatgatg tccctccttc gacgagagcc gcgtgggtta aaagagctgc
      361 ggcgcgaact cgctcagata atcgcgccag ttgagccagg tgtggccgcc ggcgctctcc
      421 tggtagatcg gtgtgaagcc gtgcttcctg aagaggtcaa ccgtcgcgac agttgtgccc
      481 agcaggaagt cgtctttgcc ggtgctgaac cagaacagct tcagcccctt cttcacgttt
      541 gcgtcggcca gtttcgccgc gtagcgcgtt tcccattctg tcggcccgga ggcggccgcc
      601 ggtggagccg gcgggccgcc gcgccctcgt tgaggaaacg cgccgaacag gccggagctg
      661 tacacaccga tgtacgcgaa ccgatcgaga ttcggaatcg cgatgttcag cgcctggttc
      721 ccgcccatcg ataagccggc aatcgcggtg ctcgcgcgat cggtcgacgc tcgatagttc
      781 ttctgcacgt agggcatcac gtcggcgacg aagtcgttca cgaacccgtc cggcccggtc
      841 gccgagagcg gggcggcgag tccacgaatg tgtcccgccg gcatgaccac gatcatcggc
      901 ctggccttcc tggcggcgat gaggttgtcc agaatgaacc ccgcgcggcc gaccgtgctc
      961 cacgagtcgt cgctgtcgcc cgcgccgtgc aggagataga agaccggata cttgcgggtg
     1021 ctggtttcgt agcccggggg tgtatacacg tgcatccgcc gaaccgcgtt gagcgccgtg
     1081 gagtggtacg tcacttcgga gacgcccccg tgggggacgt tcttcgtatc cgacgtgtcc
     1141 gatccgtcga cgtagacgag gctccacgcg ttcgtgttcg attcgctcgt tgacgggttg
     1201 cgggggtcca tcgtggcaac cccgtcgacc gtgaacctgt acctgtaagc tccagccggg
     1261 acaggcccga ccgtcgtctc ccacacaccg ttctgcccct tggtcatctc cgcgccctgg
     1321 ccgaccccgg gaatgtcgct ggccgacagc cggacggctt gcgcctgcgg agcggcaatc
     1381 ctgagcgtga tgcgacgatc cgagccgacc tcgggcgagg agaacagggg cgccaccggg
     1441 ttctgggccg tgaggtgcgg cgaggagacc gacaggaaag cggcaatcag cagcagacgt
     1501 tcacgtgtgc tggtcatagc gtgcattgta ggacgaggcc tcgtcgaagg gtagcggctc
     1561 acgagcgctc agccttgcgt cgtgccgcgg cgataggcgg agtcccggtt attatcgcga
     1621 aagttcccgg ccgccatgaa cgtcaagtcg tatctcatct ccctgcccga acggctcgtc
     1681 cgctccatcg tcggccttgg tgccggcgtc gcgcgcgagg tgggcgaggt cgcgatcccc
     1741 gacgcagtcc gccggacgcg gctctatcag aacctcgtgg acacgacgtt gaggtttctc
     1801 atcgagcagg ttggcggcgt cgaaggcgtg ttcggtgacg acgcgatgct cgcggacgat
     1861 ttcctggtca ggcgaaccgc gggcaacgcc atcgaggccc tcggtatcgt ggcgttccgg
     1921 gcgtcgcccg tctgggtgct cgcggcgctg tcggatgtgt gcggga
//
LOCUS       DBNGSH010000280         6153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1040982, whole genome shotgun sequence.
ACCESSION   DBNGSH010000280 DBNGSH010000000
VERSION     DBNGSH010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1040982"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="AC16CY_06520"
     CDS             complement(<1..793)
                     /locus_tag="AC16CY_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3353471.1"
                     /translation="MDGTSTTFFTKPELTPERREFYGRLSARNAAPLWEVLGAIIPPE
                     PRPQAVPVLWHYDEVRPLLMEAGGLLTEKEAERRVLVLENPGLRGQAKVTGSLYIGLQ
                     LILPGEVARCHRHVTSALRFVIEGSGAYTAVEGERTTMHPGDFILTPSWTYHDHGNPG
                     EVPTVWLDGLDIPIVNLFDTSFAEHYPGEIQPNTIPTDDSLVRYGANMLPVDFSPARP
                     SSPIFNYPYARSREALDRLGRNGDPHSCHGIKMRFVNPATGGYTMP"
     gene            complement(835..1710)
                     /locus_tag="AC16CY_06525"
     CDS             complement(835..1710)
                     /locus_tag="AC16CY_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3353472.1"
                     /translation="MRLCRFNEGRLGVVDGAILRDVTAALDVLPDCRYPFPSHDVLIE
                     QLDRVRERASALADRAPSIPVDRATLLSPVASPGKIVAAPLNYRKHLDEVRDDVALHQ
                     NNPARAQTVQTAGLFLKATSSLVGPGEGVVIRNTDRRTDHEVELAVVIGRRANRVSRQ
                     EAMAHVAGYAIGLDISVRGPEDRSFRKSPDSYSVLGPWLVTADEVPDPGSLDLRIDVN
                     GETRQQSNTRHMILSVAELIELASSMYTLHPGDVIFTGTPEGVSPIAPGDAIVATIAR
                     IGSMSVTARADVAMT"
     gene            1926..3275
                     /locus_tag="AC16CY_06530"
     CDS             1926..3275
                     /locus_tag="AC16CY_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3353473.1"
                     /translation="MTTSARTAGTINISDVLGNSTIGPLHMRVFVLTMASLIMDGFDV
                     QAMSYVAPAVVADWGIGRDALRYVFPAANAGVLVGSLVFSVVADKIGRRPVLVGATLF
                     FAVMSLATATAQDVTQLLWLRFIAGIGMGCIIPNSTALIGEFSPARLRVTLMMCITVG
                     FTAGAAIGGFVAAWMIPAFGWRSVFVFGGAVPLVVALAMLWGLPESLQFLAVRRRSRP
                     TLVRWLKELDPRMQVDDSTQLIAQEQNREGAPFVHLFRDGRGASTILLWVINFMNLLV
                     LYSVTSWLPLLINAMGYETRTAVLIGAVLQTGGTIGAFGLAWLIARGGFMPMLTGTFA
                     VATLSIAFIGQPGLSLTALGVIVFVAGWCVIGGQPGINALSATYYPTSLRSTGIGWGL
                     GIGRIGAIVGPYIGGVLIGQKWTEQQLFWAAAAPALVTTLTFVMLSFTMKLPEKART"
     gene            3286..4200
                     /locus_tag="AC16CY_06535"
     CDS             3286..4200
                     /locus_tag="AC16CY_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3353474.1"
                     /translation="MRYATFSDLSNPTPRLGVVRGDRIVDAAAVIGAGWRGPAPTSLL
                     DLIRQGPEAWQRMGELTDAAPDASFRPLDDVALDAPIPRPLKNVFCLGRNYIAHAEEA
                     ARARGQEVKIPTVPVIFTKAPTTVTGPFDDILVERSVTQQVDWEVELGLVIGVTGRNI
                     PRANALRHVFGYMVVNDLSARDLQQQHMQWFKGKSLDGFCPMGPVLVTADEFGDPQSK
                     RLQLRVNGASKQDGSTAQMIFPVDAIIEWISKGLTLEAGDIIATGTPDGVGMARTPQE
                     FLIDNDVVEAEVEGIGVLRNRIMAREHW"
     gene            4208..4816
                     /locus_tag="AC16CY_06540"
     CDS             4208..4816
                     /locus_tag="AC16CY_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3353475.1"
                     /translation="MAIDQDSAAIREVIENWALWRDAGDWDRFATVWHEDGWMTATWF
                     QGPARRFIEVSKEGFNRGVSILHFLGGFTCDVAANRAIAQTKMTINQRASVDGVLVDV
                     VCTGRFYDFLEKRGRWAIVRRQPIYEKDRLDPVDPSARLSLDAEHLARFPEGYRHLGY
                     LQSRNGFSVKTDLPGLRGPAVELLYAEGAAWLAGSPSPGRPL"
     gene            4827..5828
                     /locus_tag="AC16CY_06545"
     CDS             4827..5828
                     /locus_tag="AC16CY_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3353476.1"
                     /translation="MHTRKLGPFQSSAIGLGGMCLSHAYGTPPDPEAAAAVLLKALDL
                     GYTHIDSAALYGFGANESLIGRVLGGRARTFVLATKGGMFRNEQGQREIDGRPDAIRR
                     HCEESLGRLRTDVIDLYYLHRWDKRLPVEESVGALADLVREGKVRAIGLSEVSATTLR
                     KAHAVHPIAAVQTEYSLWTRNAEVAVLKTCRELGVSFVAFSPLARGFFGGQLRDVSTL
                     PPKDIRLAMPRFQGEHFARNLALLNGLSAVAAENGCTMAQAALAWVLAQGPDIIPIPG
                     TTRLDHLEENAAAESVRLSPAALERLGALINATTVSGARYNATVQQEIDTEEIAASV"
     gene            5868..>6153
                     /locus_tag="AC16CY_06550"
     CDS             5868..>6153
                     /locus_tag="AC16CY_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3353477.1"
                     /translation="MYQVTPGWLAFHPNPSVPRFKVPPGSVDAHCHVFGPGNQFPYAP
                     ERKYTPCDASKHQLFALRDHLGFARNVIVQATCHGSDNRALVDALLASGGR"
BASE COUNT          988 a         2049 c         2093 g         1023 t
ORIGIN      
        1 tgggcatcgt gtacccgccc gtggcgggat tcacgaagcg catcttgatg ccgtggcacg
       61 agtgaggatc gccgttccgg cccagccggt cgagagcctc gcggctgcgc gcgtacgggt
      121 agttgaaaat cggcgacgag ggcctcgctg gcgagaagtc caccggcagc atgttggcgc
      181 cgtagcgcac gagcgagtcg tcggtgggga tggtgttcgg ctggatctcc cccgggtagt
      241 gctcggcaaa gctggtgtcg aacaggttga cgatcgggat gtcgaggccg tcgagccaga
      301 cggtgggcac ctcgccaggg ttgccgtgat cgtgataggt ccacgacggc gtcaggatga
      361 agtcgcccgg gtgcatcgtc gtccgctcgc cttcgacggc cgtgtaggcc ccggaaccct
      421 cgatgacgaa tcggagcgcg gaggtcacgt ggcgatgaca gcgcgcgacc tcgccaggca
      481 ggatcaattg caacccgatg tacaggcttc ccgtgacctt ggcctgcccg cgcagccccg
      541 ggttctcgag gacgagcacg cggcgctcgg cttccttctc cgtcagcagg ccgcccgcct
      601 ccatcagcag cggccgcacc tcgtcgtagt gccacaggac gggcacggcc tgcggacgcg
      661 gctcgggcgg aatgatcgcg cccagcacct cccacaacgg cgccgcgttc cgggcactga
      721 ggcggccgta gaactcacgc cgctcggggg tcagctcggg cttggtaaag aaggtcgtgc
      781 tcgtcccgtc catggccaga tcctactctc gtccggcctc gacggacgcc tgcgtcaggt
      841 catggcgacg tcggcgcgcg ccgtgaccga catgctgccg atgcgggcaa tggtggcgac
      901 aatcgcgtcg cccggcgcga tcgggctgac gccttccggt gtgccggtga agatgacgtc
      961 gccagggtgc agcgtgtaca tcgacgacgc cagctcgatc agttcggcaa cggagagaat
     1021 catgtgccgg gtattcgact gctggcgcgt ctcgccgttc acgtcgattc gaaggtcgag
     1081 cgatccagga tccggcacct cgtcggccgt cacgagccac gggcccagca cgctgtagct
     1141 gtccggcgac ttgcggaagc tgcggtcctc ggggccgcga accgagatgt cgagcccgat
     1201 ggcgtagccc gccacgtgcg ccatcgcctc ctgccgcgac acccgattgg cgcgccgtcc
     1261 gatcacgacg gccagctcga cttcgtggtc cgtccgccgg tcggtgttgc gaatcaccac
     1321 gccctcgccg ggtccaacga gagagctggt tgccttgagg aagagcccgg cagtctgaac
     1381 cgtctgcgcg cgggcgggat tgttctggtg aagcgcgacg tcgtcgcgca cctcgtcgag
     1441 gtgcttccgg tagttgaggg gcgcagcgac gatcttgccc ggactggcga cgggactgag
     1501 cagcgtcgcg cgatcgacgg gtatcgacgg cgcgcgatcc gccagcgcgc tggcgcgctc
     1561 cctcacccga tcgagctgct cgatcagtac gtcgtgcgac ggaaacgggt accggcaatc
     1621 cggaaggacg tcgagcgcag cggtcacgtc cctgaggatc gcgccgtcca cgacgccaag
     1681 acgcccctcg ttgaagcggc acaagcgcat gcaaatcgat ccagtcggcg cgggtcatca
     1741 gccacggagc agggccgacg ccgagcgacg tgcttcgcag cttccggccg aggggtggcg
     1801 acggcaactg gaggacgatc cttgccagcg gtcgccgggc cggtagccga gtatacttcg
     1861 cggggcaaaa aggctgggaa ctggcataac agttatgccg gcgaccgcgc gtccaggagc
     1921 tcgtgatgac cacgtccgcc cgtacggccg gcaccatcaa catcagcgac gtgctcggca
     1981 acagcaccat cggtccgctg cacatgcgcg tcttcgtcct cacgatggcc tcgctcatca
     2041 tggacggctt cgacgtgcag gcgatgagct acgtcgcgcc cgccgtcgtc gccgactggg
     2101 ggatcgggag ggacgcgctg cgctacgtct tccccgccgc caatgccggc gtcctggtcg
     2161 gctcgctcgt gttcagcgtc gtggccgaca agatcggccg gcggcccgtg ctggtcggcg
     2221 cgaccttgtt cttcgccgtg atgagcctgg ccacggcaac ggcacaggac gtcacccagc
     2281 ttctctggct gcggttcatc gccggcatcg gcatggggtg catcatcccg aactcgacgg
     2341 ccctcatcgg cgagttcagc ccggctcgtc tgcgcgtgac gctgatgatg tgcatcacgg
     2401 tcgggttcac ggcgggcgcc gcgatcggcg gcttcgtcgc cgcctggatg attcctgcct
     2461 tcggctggcg gtcggtcttc gtcttcgggg gcgccgtgcc gctggtcgtc gcgctggcga
     2521 tgctctgggg cctgccggag tcgctgcagt tcctcgcggt gcggcggcgg agccggccca
     2581 cgctcgtgcg ctggctgaag gagctggatc cgcgcatgca ggtcgacgac tcgacgcaat
     2641 tgatcgcgca ggaacaaaac cgcgagggcg cgcccttcgt gcacctgttc cgggacggac
     2701 gcggcgcgtc cacgatcctg ctgtgggtca tcaacttcat gaacctgctg gtgctgtact
     2761 cggtgaccag ctggctgccg ctgctcatca acgccatggg ctacgaaacg cggacggccg
     2821 tgctcatcgg cgcggtgctg cagaccggcg gcacgatcgg cgccttcggc ctcgcctggc
     2881 tgatcgcgcg cggcggcttc atgccgatgc tcaccggcac atttgcggtc gcgacgctga
     2941 gcatcgcctt catcgggcag ccgggcctct cgctgaccgc gctcggcgtg atcgtgttcg
     3001 ttgccggctg gtgcgtcatc ggcggccagc ctggcatcaa cgcgctctcg gcgacctact
     3061 accccacgtc gctgcggtcg accggcatag ggtggggcct cggcatcggg cgcatcggcg
     3121 ccatcgtcgg gccctacatc ggcggtgtac tgatcggtca gaagtggacg gagcagcagc
     3181 tgttctgggc ggcggcggcg cccgcgctcg tcaccacgct gacgttcgtg atgctgtcgt
     3241 tcacgatgaa gctgcccgag aaagcgcgga cctgacccgc ccacgatgag atacgcgacg
     3301 ttttccgacc tctccaaccc cactcctcgc ctgggcgtcg tgcgcggcga ccgcatcgtc
     3361 gacgcggcgg ccgtgattgg ggcggggtgg cgcgggcccg ctcccacgag cctgctcgac
     3421 ctgattcgac agggccccga agcctggcag cggatgggcg agctgacgga cgcagcccca
     3481 gacgcctcct ttcgtcctct cgacgacgtc gcgctggacg cgccgattcc acgtccgctc
     3541 aagaacgtct tctgcctcgg gcgcaactac atcgctcatg ccgaagaagc ggccagggcg
     3601 cgcggacagg aagtgaagat cccgaccgtg ccggtcatct tcacgaaggc gcccacgacc
     3661 gtgaccgggc ccttcgacga cattctcgtg gagcgcagcg tcacgcagca ggtcgattgg
     3721 gaggtcgagc tggggctcgt catcggcgtg acggggcgta acattccgcg cgccaacgcg
     3781 cttcggcacg tcttcggcta catggtcgtc aacgacctga gcgcgcgcga cctgcagcag
     3841 cagcacatgc agtggttcaa gggcaagagc ctcgacggct tctgtcccat ggggccggtc
     3901 ctggtcaccg cggacgagtt cggcgacccg caatccaagc gcctccagct gcgcgtgaac
     3961 ggcgcgtcga agcaggacgg aagcacggca cagatgatct tcccggttga cgccatcatc
     4021 gaatggatct ccaaggggct gaccctcgag gccggcgata tcatcgccac cggtactccg
     4081 gacggggttg gaatggcacg cacgcctcag gagtttctga tcgacaacga cgtcgtggag
     4141 gccgaggtcg agggaatcgg cgtgctgcgc aatcggatca tggcaagaga gcactggtga
     4201 tcctcgattg gctatcgacc aagacagcgc ggcgatccgg gaggtcatcg aaaactgggc
     4261 gctctggcgg gacgccggcg actgggatcg cttcgccacc gtgtggcacg aggacggatg
     4321 gatgacggca acctggtttc agggcccggc caggcgcttc atcgaggtca gcaaggaagg
     4381 attcaatcgg ggcgtcagca tcctgcactt tctcggcggc ttcacctgcg acgtggccgc
     4441 caaccgggcg atcgcccaga cgaagatgac catcaaccag cgcgcaagtg tggacggcgt
     4501 gctcgtggac gtcgtgtgca cgggccgctt ctacgacttc ctcgaaaagc gcggccgctg
     4561 ggccatcgtc cgccgtcagc cgatctacga aaaggaccgc ttggatccgg tggacccctc
     4621 cgcgcggctg tcgctcgacg cggagcatct cgcgcgcttc ccggaaggct accggcacct
     4681 gggctatctg cagtcgagga acggcttcag cgtgaagacc gatcttcccg gcctgcgcgg
     4741 gccggcagtc gaactgttgt acgcggaagg cgccgcgtgg cttgccggat cgccgagccc
     4801 gggacggccg ctataaagta gacgggatgc acacgcggaa actggggccg ttccagtcct
     4861 cagccatcgg gctcggcggc atgtgcctgt ctcacgcata cggaacgccg cccgatccgg
     4921 aagcggcggc ggcggtgctg ctgaaggcgc tcgatctcgg atacacgcac atcgactcgg
     4981 cagcgctcta cggcttcgga gccaatgaaa gcctcatcgg ccgcgtgctc ggcggccgcg
     5041 cgcgaacctt cgtcctggcc accaagggcg gcatgttccg caacgaacag gggcagcgcg
     5101 agatcgacgg ccggccggac gcgatccggc gtcactgcga ggagagcctc ggccgcctgc
     5161 ggaccgacgt catcgacctg tactacctgc accggtggga caaacgactg ccggtcgagg
     5221 aaagcgtggg ggcgctcgcg gacctcgtgc gcgagggcaa ggtccgggca atcggcctgt
     5281 cggaagtctc ggccacgaca cttcgaaagg cgcatgccgt acatccaatc gccgcggtgc
     5341 agaccgaata ttcgctctgg acgcgaaacg ccgaggtcgc cgtgctgaag acgtgccgcg
     5401 agctgggtgt gtcctttgtg gcgttcagcc cgctggcgcg aggcttcttc ggcggtcagc
     5461 tgcgcgacgt ctccacgctg ccgccgaagg acatccggct cgcaatgccg cgtttccagg
     5521 gcgagcactt cgccaggaac ctcgcgctgc tgaacgggct gtcggcggtt gccgccgaga
     5581 acggatgcac gatggcgcag gccgcgctgg cctgggtgct ggcgcaaggt cccgacatca
     5641 ttcccattcc cggtacgacg cgcctcgacc acctggagga gaacgcggcg gccgagtccg
     5701 tgcggctctc ccccgccgcg ctcgaaaggc tcggtgcact gatcaacgcc acgacggtgt
     5761 cgggcgcgcg ctacaacgcc accgtccagc aggagatcga caccgaagag atcgcggcca
     5821 gcgtgtgagt ttgagtcttg agttttgagt tttgagtctt gaattacatg tatcaggtaa
     5881 cccctggctg gctcgccttt caccccaatc cttcggtgcc ccgcttcaag gtgccccccg
     5941 gctcggtgga tgcgcactgc cacgtcttcg ggccgggcaa ccagtttccc tacgccccgg
     6001 agcgcaagta cacgccgtgc gatgcatcga agcaccagct cttcgcgctg cgcgaccacc
     6061 tgggcttcgc gagaaacgtc atcgtgcagg ccacctgcca cggcagcgac aaccgcgcgc
     6121 tggtcgatgc gctcctggct tcgggcggcc gcg
//
LOCUS       DBNGSH010000281         4657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2229666, whole genome shotgun sequence.
ACCESSION   DBNGSH010000281 DBNGSH010000000
VERSION     DBNGSH010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2229666"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /gene="rpe"
                     /locus_tag="AC16CY_06555"
     CDS             <1..574
                     /gene="rpe"
                     /locus_tag="AC16CY_06555"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006721218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3353478.1"
                     /translation="VDVMDGHFVPNITIGPPVVRAIKRVATRPLDVHLMIEHPDRYIE
                     DFVSAGAAMVSVHVEAVPHLHRTVSLIRKLGAKAGVVINPATPVSSLTDIAKDVDFVL
                     VMSVNPGFAGQAFIPQSLEKLRRVVAALAAAGSSALIEIDGGIDQSNAADVVAAGANI
                     LVAGQAIFGTPDAEAATRALRAAAGRTAGG"
     gene            567..959
                     /locus_tag="AC16CY_06560"
     CDS             567..959
                     /locus_tag="AC16CY_06560"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012685112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3353479.1"
                     /translation="MVSRTRLRVRYADTDQMGVVYYANYLVWFEVGRTEWLRDAGWSY
                     REMEDEGVRLPVIEASCEYRQPLRYDDEVEIRTQATAMSPVRVRFDYEVVQLPADVVV
                     ADGRTVHAAIGREGRPCRLPSRVRDMLK"
     gene            1091..1951
                     /locus_tag="AC16CY_06565"
     CDS             1091..1951
                     /locus_tag="AC16CY_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3353480.1"
                     /translation="MNHTSLVPRPRRSRAGIVAAWLWAVALVAAAAGCGGNRVPNPAE
                     TANPDRFLFERGTAALADRKWADAREYFRQVVDNYPGSPHRADAKLGIGDTFLGEDSA
                     ESLVLAANEYREFLSFFPTNPRADYAQFKLGMTHFEQMRAPERDQSETRAALQEFQAF
                     FDRYGPVSSLTPEVRMKWREARDRLSRHELSVGITYFRISWFVGAIPRFRTILKDDPE
                     FTGRDEVYYYLAESLARSTPPRIPEAIPYFDRLIAEYETSEHLDDAKRRLQELKAQLD
                     PKTQQPSKTQ"
     gene            2096..2878
                     /locus_tag="AC16CY_06570"
     CDS             2096..2878
                     /locus_tag="AC16CY_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353481.1"
                     /translation="MTSRPTVGAVVVAGLLAGSTLTAQRGPVTPASKLPPEILSLACA
                     PTALFAPPDNTLRITGGQDSFYKDQYMPGDLLTISAGAKHGIQVGQEFYTKRVLILRK
                     ERVTNETPATLMTTGWIRVYAVDDEMSLATVTHACDGINVGDILEPFVLPTMPSPPAI
                     RYKAQRDNYGHVLTGNDRRTSFGKGDYLLIDRGSDHGVSPGSLFVLYRNKEVEGNFLY
                     DLGEAMAVSVSPDKSTLLVTVSRDAIETGDLVAIRKPVSSSK"
     gene            complement(2887..3483)
                     /gene="pgsA"
                     /locus_tag="AC16CY_06575"
     CDS             complement(2887..3483)
                     /gene="pgsA"
                     /locus_tag="AC16CY_06575"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR3353482.1"
                     /translation="MNLPNSLTLGRMFLVPLLVVVLLTEFEGRQILGVPKELVGAAIF
                     GLASLTDWLDGYIARRRRQITWLGQMLDPIADKLLTSAAFISLVQLDLAPAWMVALII
                     GREFAITALRSLAYTKGITIPASPLGKIKMASQVTAILLLILGWRPLPWLAPIGYAAL
                     WVVMLAAVVSAVDYYRRFQRLLNARVADVNVARERRVG"
     gene            3528..4586
                     /locus_tag="AC16CY_06580"
     CDS             3528..4586
                     /locus_tag="AC16CY_06580"
                     /EC_number="2.3.1.-"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3353483.1"
                     /translation="MKAILLAGGKGTRLRPLTIHTPKPIVPIFNRAFLHYQIDLLKQV
                     PEIDEVILSLNYQPRRIEEMFGDGAGLGIKIRYVVEPAPLGTAGAVKYAGDTLTESVV
                     VFNGDVMTQLDLASVIRLHRERRAKATIVLTPVENPSAYGLVETESDGTIRRFLEKPK
                     PEEITTRNINAGIYVLEPDTFDRIPSLVPWSIERSYFPSLIERGDTFVAYLYDGYWID
                     IGTPEKYTQVHHDIMDGRFLAPPFADPSASRTAISADARIEDGATIVGPVFIDDGVLV
                     KAGARVGPYAVLGRQTQVEEDAVVERAILWPNCRVGSRASVADATLGRQCHVGRNVSL
                     NGGAVLGDKTTLTDFTRA"
BASE COUNT          878 a         1517 c         1519 g          743 t
ORIGIN      
        1 tgtggacgtc atggacgggc atttcgtccc gaacatcacg atcggccccc cggttgtgcg
       61 ggccatcaag cgagtcgcga cgcggcccct cgacgtgcac ctgatgatcg agcatcccga
      121 tcggtacatc gaggacttcg tctcggcggg cgcggccatg gtctcggtac acgtcgaagc
      181 cgtgccgcat ctgcaccgca cggtctccct cattcgcaag ctcggagcga aagccggagt
      241 ggtcatcaac ccggccacgc ccgtgtcttc gctcacggac atcgcaaagg atgtggactt
      301 cgtgctcgtc atgtcggtca acccggggtt cgcgggacag gcgttcatcc cgcagagcct
      361 cgagaagctc cggcgcgtcg ttgcggcgct cgcggcggcg ggctcctcgg cgttgatcga
      421 aatcgacggc ggcatcgacc agtcgaatgc ggcggacgtg gtggccgcgg gcgcgaacat
      481 cctcgtcgct ggccaggcga tcttcgggac tccggacgcg gaggcagcca cccgcgccct
      541 gagggcggcg gccggcagga ccgccggtgg ttagccgcac gaggctgcgg gtccggtacg
      601 cggacaccga tcagatgggc gtcgtctact acgctaacta tctcgtctgg ttcgaagtcg
      661 gccggaccga atggctgcgc gatgccgggt ggagctaccg cgagatggag gacgagggcg
      721 tccgtctccc ggtcatcgaa gcatcgtgtg agtaccgaca gcccttgcgc tacgacgatg
      781 aggtcgagat caggacgcag gcgacggcca tgagcccggt gcgagtccgg ttcgactacg
      841 aagtggtaca gctgccggca gacgtcgtgg tcgccgacgg gcggacggtt cacgcggcaa
      901 tcggccgtga gggccgtccc tgccggctcc cttcgcgcgt ccgcgacatg ctcaaatgag
      961 gcgggggcag cgtctccggc cagaaacggc catccctcgt ctgtagaagc aggccgcttg
     1021 gcgggcgaaa ccggtcgagc tgtgtctaga atacagagat acttaaacgc gcgaggtctt
     1081 cgagactgga ttgaaccaca cttcactagt accccgaccc cgacggtccc gtgccgggat
     1141 cgtcgcggcg tggctctggg cggtggctct cgtcgccgcc gcggccggct gcggcggaaa
     1201 ccgcgtcccc aatccagccg agaccgcgaa ccccgatcgg ttcctcttcg agcgaggcac
     1261 ggcggcgctc gccgaccgga agtgggccga cgcgcgcgaa tactttcggc aagtcgtgga
     1321 caattaccca ggcagcccgc atcgagcgga cgccaagctc ggaatcggag acacctttct
     1381 cggcgaggac tcggcagagt ctctggttct cgccgccaac gagtacaggg agtttctcag
     1441 cttcttcccg acgaaccccc gcgccgatta cgcgcagttc aagctcggga tgacgcattt
     1501 cgagcagatg cgcgcaccgg agcgcgacca gagcgagacg agggcggcgc tgcaggagtt
     1561 ccaggcgttc ttcgatcgct acggccccgt gagctcgctg actcccgaag tccggatgaa
     1621 atggcgtgaa gcccgggatc gactcagccg ccacgagttg agcgtcggca ttacgtattt
     1681 caggataagt tggttcgttg gggcgatccc gcgatttcgc acgatcctga aagacgaccc
     1741 ggaattcacg ggccgggacg aggtgtacta ctacctggcg gaatctctgg ctcgatcaac
     1801 tcccccccgg atacctgagg ccatcccgta cttcgatcgc ctgatcgcgg agtacgaaac
     1861 gagcgagcac ctcgatgatg ccaagagacg gctccaggag ctgaaggcgc aactggaccc
     1921 gaagactcag cagccgtcga agacccaata gccccggcct gcccgccgga gcctcggcga
     1981 aggcggatgc caaactggga atacgcaatc aggggaagac gtgtgcccgt ctggcggaac
     2041 ctccggctcg gaccgataag agacctgagc aatctcgccc gacggagagc gaccgatgac
     2101 gtccagaccg accgtgggag ccgttgtggt agccggcctg ctggccggat ccacgctgac
     2161 agcgcagcgc gggccagtca cgcccgcgag caaacttcca cccgagatcc tctcgttggc
     2221 ctgcgcgccg acggccctgt tcgccccgcc tgataacacg ctccgcatca ccggcggcca
     2281 ggactccttc tacaaggacc agtacatgcc cggcgacctg ctgaccatca gcgccggcgc
     2341 caagcacggg attcaggtcg gccaggagtt ctacaccaag cgcgtgctca tcctgagaaa
     2401 agagcgcgtg accaacgaaa cgccggcgac actcatgacg accggctgga ttcgcgtcta
     2461 cgccgtggac gacgagatgt cgctcgcgac ggtcacgcat gcctgcgacg gcatcaacgt
     2521 cggcgacatc ctcgagccgt tcgtcctgcc gaccatgccc tcgccgccgg ccatcaggta
     2581 caaagcccag cgtgacaact acggccacgt gctgaccggg aacgaccgcc gaacgagctt
     2641 cggcaagggc gactacctgc tcatcgatcg cggcagcgat cacggcgtgt cgcctggttc
     2701 gctctttgtg ctgtaccgca acaaggaggt cgaggggaac ttcctctacg acctgggcga
     2761 ggccatggcc gtgagcgtgt caccggacaa atcgacgctg ctcgtgaccg tgtcgcgcga
     2821 cgcgatcgag accggcgatc tcgtggcgat ccgaaagccc gtttcgagct cgaagtaggt
     2881 cgcgcgctac ccgacccgcc gctctcgagc gacgttcaca tcggcaacgc gcgcgtttag
     2941 cagccgctgg aagcgccggt agtagtccac ggcggacacg acggcggcca gcatcacgac
     3001 ccacagcgcc gcatacccta tcggggccag ccagggcagc ggccgccatc ccagaatcag
     3061 cagcaggatc gcggtcacct gcgacgccat cttgatcttg ccgagcggcg acgccggaat
     3121 ggtgatgccc ttggtgtagg ccaggctccg cagcgcggtg atcgcgaact cgcgcccgat
     3181 gatgagggcg accatccacg cgggcgccag gtcgagctgc accagggaga tgaaggccgc
     3241 cgacgtcagg agcttgtcgg caatcggatc gagcatctgg ccgagccacg tgatttggcg
     3301 gcgccggcgc gcgatgtacc cgtcgagcca gtcggtcagc gaggcaaggc cgaaaatggc
     3361 ggccccgacc agttctttcg gcacgcccag aatctgccgg ccctcgaatt cggtcaacaa
     3421 cacgacgaca agcagcggca ccaggaacat ccgcccgagc gtgagcgaat tcggcaggtt
     3481 catggacgtg ctggcattct accatcggtg taagatcgag gattgtcatg aaagcgatcc
     3541 tcctcgccgg gggcaagggc acccgccttc ggccgttgac catccacacc cccaagccga
     3601 tcgtcccgat cttcaaccgg gcgtttctgc actaccagat cgatctgctg aagcaggtgc
     3661 ctgaaatcga cgaggtgatt ctcagcctca actatcagcc gcgtcgcatc gaggagatgt
     3721 tcggcgacgg agcggggctg ggtatcaaga tccgctacgt ggtggagccg gcgccgctcg
     3781 gcaccgccgg ggcggtcaag tacgccgggg acacgctgac cgagtcggtg gtcgtcttca
     3841 atggcgacgt catgacgcag ctggacctgg cctcggtcat ccggctgcac cgcgagcggc
     3901 gggccaaggc cacgatcgtg ctgacgccgg tggagaaccc gtccgcctac gggctagtcg
     3961 aaaccgagag cgacggcacc atcaggcgtt tcctggagaa gccgaagccc gaagagatca
     4021 ccacgcgcaa catcaacgcc ggcatctacg tgctcgagcc cgacaccttc gatcggattc
     4081 caagcctcgt cccgtggtcc atcgagcgaa gctattttcc gtcgctcatc gagcggggcg
     4141 ataccttcgt ggcgtacctc tacgacggct actggatcga tatcggcacc cctgagaagt
     4201 acacacaggt gcaccacgac atcatggacg gccggttcct ggccccgccg ttcgcggacc
     4261 cgtcagcgtc gcgaacggcg atttcggccg acgcgcggat cgaggacggc gcgacgattg
     4321 tcggccccgt gttcatcgac gacggcgtgc tggtcaaggc gggcgcgcgg gtgggtccct
     4381 acgccgtgct ggggcgtcag acacaggtcg aggaggacgc ggtcgtcgag cgggcaatcc
     4441 tgtggcccaa ctgccgcgtg ggcagccggg cgtcggtcgc cgacgcgaca ctcgggcgcc
     4501 agtgccacgt ggggcggaat gtctcgctca acgggggcgc cgttctgggc gacaagacga
     4561 cgttgacgga ctttacgagg gcgtgaaacg aaaagggggt cgggagtcat ttaatgagga
     4621 caattaaatg actcccgacc ccatttaatt gcatgcg
//
LOCUS       DBNGSH010000282         5414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_192005, whole genome shotgun sequence.
ACCESSION   DBNGSH010000282 DBNGSH010000000
VERSION     DBNGSH010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_192005"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16CY_06585"
     CDS             complement(<1..458)
                     /locus_tag="AC16CY_06585"
                     /inference="COORDINATES: protein motif:HMM:NF042769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIR3353484.1"
                     /translation="MRTTALTGFMALIAVAVPTVVVPTPGYAQLSPSATWATHAANNY
                     QIAPNLTYLTASNYESKLDIYYRRGATTPQPTVVYFHGGFWAAGTKEGAVMTILPWME
                     MGWNVVNVEYRLARVAPAPAAVEDCLCALRYLAAQAKTYSIDVSRIVVTG"
     gene            507..1592
                     /locus_tag="AC16CY_06590"
     CDS             507..1592
                     /locus_tag="AC16CY_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HIR3353485.1"
                     /translation="MKTARQACLAVIVWALASPVAAQRYAAVRDGEIVRLSDRATDTV
                     VSIVPSVGNMAVEMRVKGHQILRFPYATITDFKARPSMIGIPFMGPWANRLDEQAFYA
                     NGKRYAFDMSLGNVRGDIPIHGFLTTTDQWQVVSLRSSDNEATVTSRLEFFRQPLWMK
                     QWPFAHTIEMTYRLARGELEVATTITNLSAEAMPVAIGFHPYLQLTDSPRDEWTIAIG
                     ARTRWKLDANKVPTGETEPIGVLFPDATAAALRGYSLDDVFGDLARDADGRASMSVRG
                     KGQRLDVLVGPRYRSVVVWAPSPSGTGRGGQGPSPPGERNFICFEPMAGITNAVNLAH
                     KKKYGELQTIPPGAAWSESFWIRPSGF"
     gene            1675..3579
                     /locus_tag="AC16CY_06595"
     CDS             1675..3579
                     /locus_tag="AC16CY_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3353486.1"
                     /translation="MKTNLSLRAAWCVLPAILFVALLHPHAQGQAPAAPPAAATPPPQ
                     GPPGAGRGLPGTENGFGSFQTRCSACHGNPPIERAPSPAAIREMTPERIYESLTTGSM
                     QAQSAGLTDVQKKGLAEFMAGRPLGSAKVGDGKSMPNQCKVNPPMSDPARGGWNGWGN
                     GLANTRSQSATAAGLIASQVPRLKLKWAFGYPAGVSANAQPTVVAGRVFVGSDNGFFY
                     SLDAKTGCVYWSFEQGSIVRNAPTVGAVTGQGSARFAVFFGDGHANVYALDAQNGRLL
                     WKTKVDSHFVARITAGSTYYDGKLFVPVSSSEEWTSGHPDYSCCTSRGSVVALDANTG
                     KQIWKSWVVPEEPKPYRTMANGVTLYRPAGGAVWNAPTVDPRRGAVYFGTGDATTAPP
                     AVTTDAIMAVDINTGKLLWSYQATEGDVFMGGCNGPTKSEACPSPMGPDMDIGNSPIL
                     VTLLTGRRVLLGGTKSADVFALDPDNNGALLYRINAAGAATGGGRGGRGSIVWGGAAD
                     TERAYYGMGAAGLAAIRPATGERAWVFTPPGGRGGGLGAAPTVIPGVVFQGAGNGVLY
                     AMSAADGKVLWEFNTAQDFETVNRVPAKGGAISVSGAVVVDGMVYVGSGYAVGSGASG
                     GNVLLAFGID"
     gene            3603..3938
                     /locus_tag="AC16CY_06600"
     CDS             3603..3938
                     /locus_tag="AC16CY_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR3353487.1"
                     /translation="MSRRFIMNAGRTTKQGQQINVGKDSPEYQAIVGTLNMHPEDMKE
                     VGIASGGNVRVRSEYGEVTFKCVEGKVPQGMIFVPYGPPTCHLMGRYTDGTGMPTSKG
                     WEVEVEPVA"
     gene            4234..4521
                     /locus_tag="AC16CY_06605"
     CDS             4234..4521
                     /locus_tag="AC16CY_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353488.1"
                     /translation="MELHVEGDRIVKAKNACSLGESWFRNHTAERLYPDALVDGKPAT
                     VDEAVEAAASFLYDANMPLVYGLSNITCEAQREAVALAETIGGVVDSHTSL"
     gene            4549..>5414
                     /locus_tag="AC16CY_06610"
     CDS             4549..>5414
                     /locus_tag="AC16CY_06610"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HIR3353489.1"
                     /translation="MGGKITCTLGEVKNRADFIVYWGGNPAECHPRHFSKYTLTQKGK
                     FVPEGRKGRTMVLVDIRETPSARASDIFLQIRPGKDFEVVTTLRALLKNQRVDEAIVA
                     ETGASVETLRDLVERMKRARFGVIFFGMGLSMTRGKHMNSGSILTLTAELNAFTKFVC
                     MPMRGHGNVTGADVVLRWTTGYPFGINMSRGYPRFNPGEFSTVDLLVRGDNDAALILG
                     ADPGATMPQPGIEHLARIPTIVLDPKVTHTSRLARVHITTAVTGISAPGTAYRMDEIP
                     LPLRPALKSPYPT"
BASE COUNT          973 a         1768 c         1806 g          867 t
ORIGIN      
        1 tcgcccgtca ccacgatgcg agacacgtcg atgctgtagg tcttggcctg cgcggccaga
       61 taccggagcg cgcacagaca atcctcgacg gccgccgggg caggcgccac gcgcgcgagc
      121 cgatactcca cgttcacgac gttccagccc atttccatcc agggaaggat cgtcatcacc
      181 gcgccttcct tcgtgcccgc tgcccagaac cctccgtgaa agtagacgac ggttggctgc
      241 ggggtcgtcg cgccccggcg gtagtagata tcgagcttcg actcgtaatt gctcgccgtc
      301 aggtaggtca ggttcggcgc aatctggtag ttgttcgcgg cgtgcgtggc ccaggtcgcc
      361 gacggcgaga gctgcgcata cccgggcgtc ggcacgacga cggtcggcac agcgactgcg
      421 atcagggcca tgaatccggt gagggctgtc gttcgcatgc tcgcgagaat acactcttcc
      481 tggccctggg ctctcgccgt tcgcacatga aaaccgctcg tcaggcctgt ctcgccgtca
      541 tcgtctgggc gctcgcctcg cccgttgcgg cccagcgcta cgccgccgta cgggatggcg
      601 agatcgtaag actgagcgac cgcgcgacgg acacggttgt gtccatcgtg ccgtcggtcg
      661 gaaacatggc cgtcgagatg cgggtcaagg ggcaccagat ccttcgattc ccatatgcga
      721 ccatcaccga cttcaaggca cggccgtcga tgattggtat tccgttcatg ggaccctggg
      781 ccaatcgcct cgacgagcag gccttttacg ccaacggcaa gcgctacgcc ttcgacatga
      841 gtctgggcaa cgtccgcggc gacataccga ttcacggttt cctcaccacc acagaccagt
      901 ggcaggtcgt ctccctccga tcgtcggaca acgaggcaac cgtcacgagc cggctcgagt
      961 tcttccgtca gccactatgg atgaagcaat ggccgttcgc ccacacgatc gagatgacgt
     1021 accggctggc tcgaggcgaa ctcgaggtgg ccaccacgat caccaacctc agcgcggagg
     1081 cgatgccggt cgcaatcggt tttcatccgt atctccagtt aaccgactcg ccccgcgacg
     1141 agtggacgat cgccatcggc gcgcgcaccc ggtggaagct cgacgcgaac aaggtgccaa
     1201 ccggcgagac agaacctatc ggcgtgctgt tcccggacgc gacggcggct gcgttgcgcg
     1261 gctacagcct cgacgacgtg ttcggcgatc tggcccggga cgcggatgga cgcgcctcga
     1321 tgtcggtgcg gggcaagggg cagcggctcg atgtgctcgt cggaccccgc taccgatcgg
     1381 tggtcgtctg ggcgccgagc ccctcgggca ccgggagagg cgggcaaggc ccctcgccgc
     1441 ccggagagcg caacttcatc tgcttcgagc cgatggctgg gatcaccaac gccgtcaacc
     1501 tggcgcacaa gaagaaatac ggcgagcttc agaccatccc gcccggagct gcgtggagtg
     1561 aaagcttctg gatccggccg agcggattct gaggtgtcac gaacgtgcgg atggcgttcg
     1621 cgaatctgct atggttagcg gcggtctacc cggacacaac ctcgaggagt tccagtgaag
     1681 acaaacctgt ctctgcgcgc ggcgtggtgc gttctccccg ccattctttt cgtcgctctg
     1741 cttcatccac acgcgcaagg acaggcgccg gcggcgccgc ccgcagccgc cacaccgcca
     1801 ccgcaggggc cgcccggcgc aggacgtggt ctcccgggaa ccgagaacgg cttcggcagc
     1861 tttcagacgc gctgctcggc ctgtcacggc aatccgccga tcgagcgcgc gccctcgccc
     1921 gcggccatcc gcgagatgac gcccgagcgc atctacgagt cgctgacgac gggatcgatg
     1981 caggcacaga gcgcggggct gaccgacgtg cagaagaagg ggctcgccga gttcatggca
     2041 gggcggcccc tcggcagcgc gaaggtgggc gacggcaaga gcatgcccaa ccagtgcaag
     2101 gtgaatccgc ccatgtccga tccggcgcgc ggcggatgga acgggtgggg caacggcctg
     2161 gccaacacgc gatcacagtc ggcgaccgcc gcaggcctga tcgcctctca ggtgcctcgc
     2221 ctgaagttga agtgggcgtt cgggtatccc gcgggcgtct cggccaacgc gcagccgacc
     2281 gtcgtcgccg gccgcgtgtt cgtcggcagc gacaacggat tcttctattc tctcgacgcg
     2341 aaaaccggat gcgtctactg gtccttcgag cagggatcga tcgtgcggaa cgcgcccacg
     2401 gtcggcgccg tcaccgggca gggatccgcg cgcttcgccg tgttcttcgg tgacggccac
     2461 gccaacgtgt acgccctcga cgcacagaac gggcggctgc tctggaagac gaaagtggat
     2521 tcccacttcg tggcgcgcat tacggcgggc tcgacgtact acgacggcaa gctcttcgtg
     2581 cccgtctcct cgtccgagga gtggaccagc gggcatccgg actactcgtg ctgcacctcg
     2641 cgcggcagcg tcgtcgcgct cgacgcgaat acagggaagc agatctggaa gtcgtgggtc
     2701 gtccccgagg agccgaagcc ctatcgcacg atggccaacg gcgtgacgct gtacaggccg
     2761 gccggaggcg ccgtctggaa tgccccgacc gtcgatccgc gtcgcggcgc ggtgtacttc
     2821 ggaacgggcg acgcaacaac agcgccgccc gccgtgacga ccgacgccat catggcggtg
     2881 gatatcaaca ccgggaaact cctctggtcc tatcaggcca ccgagggcga cgtgttcatg
     2941 ggcggctgca acggacccac caagagcgag gcctgcccga gccccatggg accggacatg
     3001 gacatcggca actcgccgat tctggtcacg ctgctgaccg gcaggcgcgt cctgctcggg
     3061 ggcaccaaga gcgcggacgt gtttgcgctc gacccggaca acaacggcgc actgctctac
     3121 cggatcaacg ccgccggcgc cgccaccggc ggtgggcgtg gagggcgcgg gtccattgtg
     3181 tggggcggcg ccgccgatac cgagcgcgcg tattacggca tgggcgcagc cggattggcg
     3241 gccatccggc ctgcgactgg cgaacgcgcg tgggtcttca caccgccagg cggtcgaggc
     3301 ggcgggctcg gggcggcgcc cacggtcatc ccgggagtcg tcttccaggg ggcgggcaac
     3361 ggggtcttgt acgcgatgtc ggccgccgac ggcaaagtcc tctgggaatt caacaccgcg
     3421 caggacttcg aaacggtgaa ccgtgtgcct gccaaggggg gcgccatcag cgtgtctggc
     3481 gctgtcgtcg tggacggcat ggtgtatgtc ggctcgggct acgcggtcgg ctctggtgcg
     3541 agcggtggaa acgtactgct cgcattcggt atcgattaga tcggggtaca cttgacgggc
     3601 tcatgagccg tcgcttcatc atgaacgcgg gccgcacgac caagcagggc cagcagatca
     3661 atgtcggcaa ggactcgccg gaataccagg ccatcgtcgg cacgctgaac atgcaccccg
     3721 aggacatgaa ggaagtcggc atcgcgtcgg gcggcaacgt ccgcgtgcgc tccgaatacg
     3781 gagaggtcac gttcaagtgc gtcgaaggca aggtcccgca ggggatgatc ttcgtgccct
     3841 acggcccccc gacgtgccat ctgatgggca gatatactga cggcaccggc atgcccacgt
     3901 ccaaagggtg ggaagtcgag gtcgagccgg ttgcgtgatc tgatcatggc gaacactcca
     3961 cgctcccagc ctccgagccc gggcactcgc gtccccgatc ccgagccccg agtcctgtcg
     4021 tgggagcaga tcgagcccgg gcgctacatg cctcggcgga ctcgagggag ctacccgggc
     4081 cgggagctgg gttgaagctt cgtcgtgccg cccggtcggg cggatgagga actgaagtaa
     4141 ttcaaaaggc acaacgcaaa agtcaaaagg caaatggaac tgagcgtcgt aaagaacgcc
     4201 acctgcactt tctgcggctg cgtgtgtgac gacatggagc tgcacgtgga gggcgaccgg
     4261 atcgtcaagg cgaagaacgc gtgcagcctt ggcgagtcgt ggttccgcaa tcacacggcg
     4321 gagcgcctgt acccggacgc gctcgtggac ggcaagcccg cgacggtgga cgaggcggtc
     4381 gaggccgctg ccagcttcct gtacgacgcc aacatgccgc tcgtctacgg gctcagcaac
     4441 atcacgtgcg aggctcaacg cgaagccgtc gccctggccg aaacgatcgg aggtgtggtc
     4501 gacagccaca cctcgctttg acacggcccc actgagatag ccgcccagtt gggtggcaag
     4561 atcacatgca ctctgggcga ggtcaagaat cgggcggact tcatcgtcta ctggggcggg
     4621 aatccggccg agtgccatcc gcgccatttc agcaagtaca cgctgaccca gaaaggcaag
     4681 ttcgttcccg aggggcgcaa ggggcgcacg atggtgctcg tggacatcag ggagacgccg
     4741 agcgcccggg cgtcggacat ctttctgcag atcaggcctg gcaaggactt cgaagtcgtc
     4801 acgacgctgc gcgcgctcct gaagaatcag cgggtggacg aagccatcgt cgccgagact
     4861 ggcgccagcg tcgagacgct gcgcgacctc gtcgagcgga tgaagcgcgc ccgtttcggc
     4921 gtcatcttct tcggcatggg cctgtcgatg acgcgcggca agcacatgaa ctcggggtcg
     4981 attctgacgc tgaccgcgga gttgaacgcc ttcacgaagt tcgtctgcat gccgatgcgc
     5041 ggacacggca acgtcaccgg ggccgacgtc gtcctgcggt ggacgacggg gtatcccttc
     5101 ggcatcaaca tgtcgcgcgg gtatcccagg ttcaacccgg gagagttctc gacggtggac
     5161 ctgctggtcc gcggcgacaa cgatgccgcg ctgattcttg gcgccgaccc cggagccacg
     5221 atgccgcagc cgggcatcga gcacctcgcc aggattccga cgatcgtcct cgaccccaag
     5281 gtgacgcaca cgagccggct ggcccgcgtg cacatcacga ccgccgtgac gggcatcagc
     5341 gcgccgggaa cggcctatcg catggacgag atcccgctgc cgctgcgtcc ggcactgaag
     5401 tcgccgtatc cgac
//
LOCUS       DBNGSH010000283         6061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1380465, whole genome shotgun sequence.
ACCESSION   DBNGSH010000283 DBNGSH010000000
VERSION     DBNGSH010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1380465"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="AC16CY_06615"
     CDS             <1..436
                     /locus_tag="AC16CY_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353490.1"
                     /translation="WQDERTLKIDTDAGSQTRLLTFGPSPSQGGDWQGSSIASWDRSQ
                     SVMGAGGGFFGPPASRGGSLKVVTTRMKPGYLRKNGVPYSGDAVITEYFDRLELPNGD
                     ALLVVSTEVVDPAYLTQPFWTSTHFRKQNDASGWSPTPCSAR"
     gene            421..2277
                     /locus_tag="AC16CY_06620"
     CDS             421..2277
                     /locus_tag="AC16CY_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353491.1"
                     /translation="MLGAMTRHHLPSCQALIRIAAIACALAMSARLTGQAPAPANATA
                     PAVDLSGYWTSVLHEDSMERGAGPEIADYGGFPVNEAARLFGLSYDPSRLTLRHHQCD
                     GYVAPYQIRALGNARAWEERDANTHRLVAIHWFNQTFEGKRTIWMDGRPHPPAWAPHT
                     WMGFSTGRFVGNALRVETTHLKQGWLRRNGLPESDQATTIEFFIRHGDRLTHTVVVND
                     PVYLAEPLVKTTDFYRQPIDPQNWLFPCDDGEQILGRAPDDVPNYLFGQNPFVKEYQD
                     KHKIPLAGHLGGAPTMYPEIAARLESGADSALLRPAPGARPVSAAVDPEPRDGKVHVW
                     PLRGNVYLLTGDEGNIVVQVGDEGPVVVDTGRGRLAKETVQAIRTLSDKPIQLIVNTS
                     FHADRTGGNAALQASGDDPSVRGSFFSLQFRDAGVGATIMAHQNVSNRLVAAKAEPRA
                     VPSDTYLEERRRTFHNGDSIELFWQRNAVTDGDSLVHFRRADVIVAGNVFTTTQYPFI
                     DVANGGTIDGEIRALNDILARTVYAHEGEGGTIVVPGHGYVCDEHEVVEYRDMVVIIR
                     DRIRAMMRAGATLAQIRAARPTADYDTRYGAVTGPWTTDQFVEAIHRTLTVK"
     gene            complement(2293..2553)
                     /locus_tag="AC16CY_06625"
     CDS             complement(2293..2553)
                     /locus_tag="AC16CY_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353492.1"
                     /translation="MTTVTPISAVEEIKERLTPTLDAIDEAVRHMKRVVVRGQHAVED
                     ATAAATLTIRRRPLSAVSSAALGGALLGLLTGFGIGRLSREE"
     gene            complement(2752..3516)
                     /locus_tag="AC16CY_06630"
     CDS             complement(2752..3516)
                     /locus_tag="AC16CY_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353493.1"
                     /translation="MAKKALEAVVVSVAVVLAGATSAIAQPCGSLCADLESQEPGRPR
                     ADTQTGSVPEVGAFDWQGALVQSGLFLAAQHSFRIAQQNRREALGGPFWRDYVNSVRG
                     LQGWDDGSQSYVNYLGHPLMGGISGFIQIQNDPRGIDLEWDPGDAAYWKSRLKALGWA
                     AVYSTTFELAPWGEAGIGNDGLEPGTMGYVDLVLTPLGGFGWILLEDYLDASVIRKLE
                     ANGVTGKVRVLRVMLNPGRSVANLLRFKRPSHRDTR"
     gene            complement(3555..>6061)
                     /locus_tag="AC16CY_06635"
     CDS             complement(3555..>6061)
                     /locus_tag="AC16CY_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353494.1"
                     /translation="RAASGLPWLESVSVDVKLGVRLLFKYPGLTIVSTLALSIAIAVV
                     AGFHAGTEFMVRPTLPVPEGDRIVAVWNHDVRTSDRGEQTVGDMVTWRRQLASLQDVG
                     AFARQQRVVADANGATRLVRAAAISPSAFQMVRIPPLMGRPLVDGDERPGAAVAVVGF
                     DLWQSAFGGDAGVVGKSIRVGGLEHTVVGIMPAEFRFPVSEQLWTPLRVASAGLAPGE
                     GPAVEFSVGRLAPGVTLDEAQAELQVIGSRIAAERPGTHGQSRPRMTTYARSLLEANE
                     PGLATMMSAARVLIGIVLLVVAVNVGTLVYARDASRVGEVAVRTALGATRSRIVLQML
                     LEALVLASLAAAVGLAIMAWPLYLLREVLGIGLPQAEGLPFWFDVRLGPSTMLVVVGL
                     VLLSAALTGMLPALKLTDRATRSALQRVQAGASGLRFGTTATSVIVSQVAISVALLTV
                     GASQLRTFVDDWRSLQRDETDRAQYLAAQLRWDLAAPSGASGVQANDALRRADTWRVL
                     GRRLSQEPDVRGVTFDTFNGVRPFAPAGIAAAPAPGSSWSWIVSIEPNYFDVYETSIR
                     AGRVFDAVEATEETRRVAIVNEAFVRTVLQAGSPIGQRIHPVEPRSGRPAGESLAIVG
                     VVADSLNLEISQRGPGWVAHPTVYVPLRPTSSPIRMTVRTQGDPTPLVGRLQAIAAGI
                     DSTLVVHRPGPLENVDRVDQVLLRLYGFGVGFVIVAVLLLSTAGVYSMLSFTVSQRTR
                     EIGIRTALGASPGRVTTDVLSRAFTQIGSGTLLGLAIGYAASAGPFRLSDGLFDKGPG
                     VMIGVAAVILLAGLGACGTPLRRALRIQPTEALRTD"
BASE COUNT         1085 a         2098 c         1974 g          904 t
ORIGIN      
        1 atggcaggac gagcgcacgc tcaagatcga caccgatgcc ggctcgcaga cgcgccttct
       61 cacgtttgga ccatcaccct cgcagggcgg cgactggcag ggctcgtcga tcgcgtcgtg
      121 ggaccgctcg cagagcgtga tgggagcggg cggcgggttc ttcggccctc cggcttcacg
      181 cggcggttca ctcaaggtcg tcacgacgag gatgaagccg ggatacctgc gaaagaatgg
      241 cgtcccatac agcggtgacg ccgtcatcac cgagtacttc gaccggctcg agcttcccaa
      301 cggagacgcg ctgctcgtcg tctcaacgga agtcgtggac ccggcctacc tcacccagcc
      361 gttctggacg agcacgcact tcaggaaaca gaacgacgcg tccggctgga gcccgacgcc
      421 gtgctcggcg cgatgacgcg gcaccacctg ccctcctgcc aagccctgat ccgcatcgcg
      481 gcgatcgcgt gcgcgctcgc gatgtcggct cgcctcacgg gtcaggcgcc ggcaccggcg
      541 aacgcgacgg cgcctgcggt tgacctgtcg gggtactgga cgtcggtgct gcacgaggac
      601 tcgatggaac gcggcgccgg accagagatc gccgactacg gcggcttccc cgtcaacgaa
      661 gcggcgcgcc tgttcggact gtcctacgat ccttcgcgcc tgaccctgcg gcaccatcag
      721 tgcgacggct acgtggcgcc gtaccagatc cgcgcgctcg gcaacgcacg cgcctgggag
      781 gaacgcgacg cgaacaccca tcgcctcgtc gcgatccact ggttcaacca gacatttgaa
      841 ggcaagcgca cgatctggat ggacggccgc ccgcatccgc ctgcctgggc tccgcacacc
      901 tggatgggct tctcgaccgg acggttcgtc ggcaacgcct tgcgggtcga gacgacccat
      961 ctcaagcagg ggtggctgcg ccgcaatgga ctgcccgaga gcgatcaggc caccacgatc
     1021 gagttcttca tccggcacgg cgaccgcctg actcacacgg tggtcgtgaa cgatccggtg
     1081 tacctggccg aaccgctcgt gaagacgacg gacttctatc ggcagcccat cgatcctcag
     1141 aactggttgt tcccgtgcga tgacggcgag cagatcctgg gacgcgcccc ggatgatgtg
     1201 ccgaactacc tgttcgggca gaacccgttc gtcaaggagt accaagacaa gcacaagatc
     1261 ccgctcgcgg gtcatctcgg cggcgcgccg acgatgtatc cggagattgc tgcgcgtctc
     1321 gagagcgggg cggattcggc gctgctgcgt ccggcgcccg gggcaaggcc ggtcagtgca
     1381 gcggtcgatc ccgagccgcg tgacgggaag gtccacgtct ggccgctgcg cgggaatgtc
     1441 tacctgttga ctggcgacga aggcaacatc gtggtgcagg tcggagatga aggtcccgtg
     1501 gtcgtggaca cggggcgcgg acgactggcc aaggagaccg tccaggccat ccgcacgctg
     1561 agcgacaagc cgattcagct catcgtgaac accagctttc acgccgaccg tacgggcggc
     1621 aacgctgcgc tgcaggcgtc aggcgacgat cccagcgtgc gcggctcgtt cttctcgctg
     1681 cagttccgcg atgccggcgt cggcgcgacg atcatggccc accagaacgt gtcgaaccgg
     1741 ctggtggcag ccaaggccga accgcgcgcc gtcccgagcg acacttacct cgaggagcgc
     1801 cgccgcacgt tccacaacgg cgattccatc gagctcttct ggcagagaaa cgcggtcacc
     1861 gacggggaca gcctcgtcca cttccgccgg gcagacgtga tcgtcgccgg caacgtgttc
     1921 acgacgacgc agtatccgtt catcgacgtc gccaatggcg gcaccatcga cggggagatt
     1981 cgggcgctca acgacatcct ggcccggacg gtctacgcgc acgaaggcga agggggcacg
     2041 atagtcgttc ctggacacgg gtacgtatgc gacgagcacg aggtcgtcga gtaccgtgac
     2101 atggtcgtca tcatccggga tcggatccgc gcaatgatga gggcaggggc aacgctcgcg
     2161 cagatcaggg cggcccgccc cacggccgac tacgacacca ggtacggagc cgtcacaggg
     2221 ccgtggacga cggaccagtt cgtcgaagcc atccatcgga ccctgacggt gaagtaacgg
     2281 ggtcctctgt gctcactcct cgcgactcag tcgcccgatt ccgaagccag tcagcagtcc
     2341 aagcagtgcg cctccgaggg ccgcggacga caccgcactc agcggccgcc gcctgatggt
     2401 cagggttgcc gcagcggtgg cgtcctcgac cgcatgctgg ccgcggacga cgacgcgctt
     2461 catgtgacgc accgcttcgt cgatcgcgtc gagggtcggc gtcaaccgct ccttgatttc
     2521 ttccaccgct gagattgggg tcacagtggt catgagaacc tccaccccag ccgaccgcaa
     2581 gagtcgcgcc actggcgtgc gctcgatcca aaggacatcg ctgcgcggtg gagggctctg
     2641 tgccggaaag cgccgccccg ttttacgcga agcttccaat gcttcattga aacgctgcca
     2701 cgctgtcccg gctcgaccgc ggggccgtcg aggcagacgt ggaggcgtga gtcagcgagt
     2761 gtcgcgatgc gagggccgct tgaaccgcag cagattcgcg acactgcgtc ccgggttgag
     2821 catgacgcgc agaaccctga cctttcctgt cacgccgttg gcctcgagct tcctgatgac
     2881 ggacgcgtcg agatagtctt ccagcaggat ccagccgaag ccgccgagcg gcgtcagcac
     2941 gagatcgacg tagcccatcg tgccgggctc caggccgtca ttgccgatgc ccgcttcgcc
     3001 ccagggcgcc agctcgaagg tcgtgctgta caccgccgcc catccgagcg ctttcaagcg
     3061 gctcttccaa tacgcggcgt cacccggatc ccactcgaga tcgatgccgc gaggatcgtt
     3121 ctggatctgg atgaacccgc tgatgccacc catgagtgga tgtccgaggt agttgacata
     3181 cgactggctg ccatcgtccc atccctggag tccgcggacc gagttcacgt agtcgcgcca
     3241 gaagggacct ccaagcgcct ctctcctgtt ctgctgcgcg atgcgaaacg agtgctgcgc
     3301 tgcgaggaac aagccggact gcacgagcgc accttgccag tcgaaggcgc caacctcggg
     3361 cacgctcccg gtctgggtgt cggcgcgagg gcggccaggt tcctgcgatt caaggtccgc
     3421 gcagaggctc ccgcagggct gggcgatcgc gcttgtggct ccggccagca cgacagcaac
     3481 ggacacgacg acagcctcca gcgccttctt cgccatgggt gtcgatcgat cttacggcga
     3541 tgtgggcgcg gcggtcagtc cgtgcgcagt gcttcagtcg gctgaatccg gagtgcgcgt
     3601 ctcaagggcg tcccgcaggc gccgaggccg gccagcagaa tgacggccgc aacgccgatc
     3661 atgactccag gccctttgtc gaagagcccg tccgacaacc tgaacggtcc ggccgacgcc
     3721 gcgtatccga tcgccagccc cagcagtgtg cccgagccga tctgcgtgaa cgcgcgcgac
     3781 agaacgtccg tcgtcacgcg gcccgggctc gcgccgagcg ccgttcgtat cccgatctcc
     3841 cgggtccgtt gcgagaccgt gaaggacagc atcgagtaca cgcccgcggt ggacagcagc
     3901 agtaccgcca ctatcacgaa accgacgccg aagccgtaaa ggcgcaggag gacctggtca
     3961 acgcggtcca cattctcgag tggccccggc cgatgaacga ccagggtcga atcgatgccc
     4021 gcggcgatcg cctgcagcct gcccacgagc ggcgtcgggt cgccctgcgt gcggacggtc
     4081 atgcggattg gcgacgaggt cggccgcagc gggacgtaga ccgtcgggtg ggccacccac
     4141 ccggggccgc gctgggaaat ttcgagattc agggagtcgg caacgacgcc cacgatcgcc
     4201 agcgattcgc ctgccgggcg gccgctccgc ggctcgacag gatgaatgcg ctgcccgatc
     4261 ggtgaaccgg cttgaagaac cgtccggacg aaggcctcgt tcacgatcgc cacgcgtctc
     4321 gtctcctccg tcgcctccac cgcatcgaac acgcggccgg cgcggatcga cgtctcgtag
     4381 acgtcgaagt agttcggctc gatcgacacg atccacgacc agctcgagcc cggggcgggg
     4441 gcggcggcga tcccggccgg cgcaaacggg cgcaccccat tgaacgtgtc gaatgtcact
     4501 ccgcgcacgt cgggctcctg ggaaagccgc cggccaagta cgcgccacgt gtcggcccgc
     4561 cgaagggcgt cgttggcttg cactccagat gcgccgctcg gcgcagccag gtcccagcga
     4621 agctgcgccg ccaggtactg cgcccgatcg gtctcgtcgc gctggagcga cctccagtcg
     4681 tcgacgaagg tacgcaactg cgatgcgccc acggtgagaa gtgccactga gatcgccacc
     4741 tgcgagacga tgacggacgt ggccgtggtg ccgaatcgca ggcccgaagc tcccgcctgc
     4801 acgcgctgca atgcggaccg cgtggcacgg tcggtcagct tcaatgcggg cagcatcccg
     4861 gtcagtgccg ccgacaagag aacgaggccc acaaccacca gcatggtcga gggccccagt
     4921 cgcacgtcga accagaaggg cagaccttca gcctgcggga ggccgatgcc caacacttcc
     4981 ctgaggaggt acagcggcca cgccatgatc gccaggccga cggccgcggc gagcgaagcg
     5041 agtaccagcg cttccagcag catctgaagc acgatgcggg atcgagtggc gccgagggcc
     5101 gtccgcacgg caacctctcc gacgcgcgag gcatcccgcg cgtacaccag cgtgccgacg
     5161 ttcacggcca cgacgaggag gacgatgccg atcagcacgc gcgccgcgct catcatcgtc
     5221 gcgagaccgg gctcgttcgc ttcgaggagc gaccgggcgt aagtggtcat tctgggacga
     5281 gactggccgt gcgttcccgg cctctcggct gcgatcctgg acccgatgac ttgcagttcc
     5341 gcctgcgcct cgtccaacgt cacgcccggc gcaagccggc ccaccgagaa ctccacggcc
     5401 ggcccttcac ccggcgcgag accagcgctc gcgacgcgca acggagtcca gagttgttcg
     5461 ctgacgggga agcggaactc ggccggcatg atcccgacga cggtgtgttc caggccgccc
     5521 acacgaatgg acttccccac caccccggcg tctccgccaa acgcggactg ccagaggtcg
     5581 aatccgacaa cggccacagc agcccccggc cgctcgtcgc catcgacgag cggtcgtccc
     5641 atcagcggag gaatgcgcac catctgaaag gccgacgggc tgatcgccgc ggcccgcacc
     5701 agccgcgtcg cgccgttcgc atcggccacg acccgctgct gccgcgcaaa ggcgccaacg
     5761 tcctgcagcg acgccagctg acggcgccac gtcaccatgt cgccgacggt ctgctcgccg
     5821 cgatccgacg ttcggacgtc gtgattccag actgcgacaa ttcgatcgcc ctcaggtacg
     5881 ggcagcgtcg gccggaccat gaactccgtc cccgcgtgga atccggcgac cacggcgatc
     5941 gcgatggaca gggccagcgt cgaaacgatt gtcaggcccg gatacttgaa cagcaggcgc
     6001 acgcccagct tgacgtccac cgagacggac tcgagccacg gcaggccaga tgccgcacgg
     6061 c
//
LOCUS       DBNGSH010000284         5879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4267169, whole genome shotgun sequence.
ACCESSION   DBNGSH010000284 DBNGSH010000000
VERSION     DBNGSH010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4267169"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /gene="lipB"
                     /locus_tag="AC16CY_06640"
     CDS             <1..609
                     /gene="lipB"
                     /locus_tag="AC16CY_06640"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3353495.1"
                     /translation="ALEMQRALVEERRAGRVPDLLLLLQHPPVITLGVRADAARANIV
                     AGADRLAGLGIDVCETGRGGDVTYHGPGQVVGYPILDLRPDRCDVHKYVRDLEEVMIR
                     VCGDYGVHAERVRGLTGTWIGAGKIGAIGIRISRWITSHGFAFNVSTDLEHFQLIVPC
                     GIADRGVTSLEKATGRAVAIGEVEDAIARHFAEIFARDRGSG"
     gene            complement(643..1968)
                     /locus_tag="AC16CY_06645"
     CDS             complement(643..1968)
                     /locus_tag="AC16CY_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3353496.1"
                     /translation="MEIVIPIAIILLLVLANGLFVAAEFAIVGAPRSNIEHQASLGSG
                     LALRVLRILEDPKLQDRYIATTQVGISVASLGLGMYGEHWLADQMQPWFASLERTGWI
                     LSHTVASVVAVAILTYLHIVLGEMVPKALALQQAQTTVLYVSPIIRALQHAILPLIVG
                     LNEIGNKVLWLVGIERREVDRERYHTAEELEFIVRESQAGGLLRGESGEILRDLFEFG
                     DRTAGEVMVPRVLLAGIPVGAEPDELRAIVRAHAHTRYLVYSGDLDNIVGSLHIKEIL
                     RHLISNRSVAATDARALPYVPGPAQLDQVLTAMRRMRAQMAVVMDQHGGTAGVLTIED
                     LFEEVVGDIEEGRARGPIVVEADGRVRVRGTVRLDEAGEALGRSLEHSKVTTVSGLVL
                     LLLGRPAITGDSVTWNTVRIDVLSVASRGVREAMLSIPKSGDDVRSVRL"
     gene            complement(1968..3284)
                     /locus_tag="AC16CY_06650"
     CDS             complement(1968..3284)
                     /locus_tag="AC16CY_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3353497.1"
                     /translation="MVPAAIPVIAALILVNALYVGAEFAAVSVRRSRLHQLAEGGNPF
                     AAWLLPLIESPAALDRYIAACQIGITLSSLVLGAYAQATIAVWLAPLVARFGGLQDVV
                     AQSTSTAVVLLVLTVAQTVFAELVPKSLALQYPTQTSLYTLVPMVASQWVYRPFITWL
                     NGTGLLILRLMGAQHQAHRHIHSPEEIELLIAESRDGGLLEPDEHRRLQRALQLNLRQ
                     ARQLMVPRRRIAAIEVNTPLAEVVSIVTQSPYSRMPVYRESIDNIVGILRTKDLARWF
                     VEGAPGTKLADLIRPVPSVHESVTADRILKDLRERRSHQALVVDEFGGTAGLLTLADV
                     LTELIGHVGDEFKSEPGAETLSDGRVRLPGEMSVVEAASVLQAVWDTDAATIGGMVTE
                     ALGHLPVAGETIAIGEFEFEVERVVDRTPQSLAVRPLASVISGGAS"
     gene            3464..4090
                     /locus_tag="AC16CY_06655"
     CDS             3464..4090
                     /locus_tag="AC16CY_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3353498.1"
                     /translation="MDRTQDRALIAGLQSGSHESLRQIADLYGPRVYQLAMRHMKNRE
                     DAEEVTQDVLMKVYRKAGSFRGDAALSSWIYRITFNTAMSRLRQRYSARAAEQPRDNA
                     RFEAWDQPSHVQGEPADWSHMPDDELLRLELRQAVAAAIRDLPEIYRQPIILRDLEGL
                     TTEEASTRLKLKDETLKSRLHRGRLLLRERLRVFATGLTLHRPTTAYS"
     gene            complement(4135..4398)
                     /locus_tag="AC16CY_06660"
     CDS             complement(4135..4398)
                     /locus_tag="AC16CY_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353499.1"
                     /translation="MRRHARLVVVAALLVAAGWSVGAQRAVADFEINVEVPRGQVTVS
                     CARGCDWPREATSRLHTTSLVCETERCRFMFNGYGRITLGMPR"
     gene            complement(4413..>5879)
                     /locus_tag="AC16CY_06665"
     CDS             complement(4413..>5879)
                     /locus_tag="AC16CY_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3353500.1"
                     /translation="ADIASGALLEHIRFLSSDDLKGRANGTPGLDSAGDYVAAQFKAV
                     GLQPGGAGGDWFQPFELVAGLKVGSVNTLSISGKGRKASFVVGTSYYPLSAPSNEDVS
                     KPSADLRGLPVVFAGYGLVVPGIYDDYARIDVNGKAVLILSHEPQEHDPNSRLNGTRP
                     MQQTTVAAKAAAARVRGAKALIVVLDPTHRVDDAPYGLFGADPDAEDHGIPLLRIRRA
                     EAQPLVEAWRLDETAREIDADLMPRSRDLPDARVDYVEHLAKNKHTVRNVVGVLPGSD
                     AARAREAIVIGAHYDHVGFGGRLSQSPDKTGEIHNGADDNASGTASIIEIARAAAADR
                     ARFPRTLIFVAFAGEERGLLGSAHYVSQPRVPIENTVAMLNLDMVGRANGAVDVSGLE
                     ASPSMEADLEAAVKASGSTLKVRREGPGAGRSDDYSFIARRIPAINFFTGFHDDYHRP
                     GDDWQKIDVPGTTRVARLALELAASLANRETKPEFVSK"
BASE COUNT          973 a         1975 c         1980 g          951 t
ORIGIN      
        1 gccctcgaga tgcagcgcgc gctcgtggaa gagcggcgcg ccggccgcgt tcccgatctc
       61 cttctccttc ttcagcaccc gcccgtcatc acgctcggcg ttcgcgccga cgccgcgcgc
      121 gccaacatcg tcgccggcgc cgacaggctg gccggactcg ggattgacgt gtgcgagaca
      181 gggcggggcg gcgacgtcac gtatcacggg ccgggccagg tcgtcggcta cccgatcctc
      241 gatcttcggc cggaccgctg cgacgtgcac aagtacgtgc gggacctcga ggaggtcatg
      301 atccgcgtct gcggggacta cggcgtgcac gcggaacgcg tgcgcggcct cacgggtacg
      361 tggattggcg cgggcaagat cggcgcgatc gggatccgga tctcgcgctg gatcacgagc
      421 cacgggtttg ccttcaacgt gagcacggac ctcgagcact tccagctgat cgtgccgtgc
      481 gggatcgcgg accggggcgt cacgtcgctc gaaaaggcca cggggcgggc ggtcgccatt
      541 ggcgaagtcg aggacgcgat cgcgcgccat ttcgcggaga tcttcgcacg tgatcgcggg
      601 tccggctgaa gccggacact acgtacgtcg tcgcgagtcc ggctaaagcc ggacactacg
      661 tacgtcgtcg ccggacttgg ggatcgacaa catcgcttcc cgcacgccgc ggctggcaac
      721 cgacaagacg tcgatccgca cggtgttcca ggtcacgctg tcgcccgtga tcgccggccg
      781 gccgagcagc agcagcacca gtccgctgac cgtcgtgacc ttcgagtgct ccagcgatcg
      841 gccgagcgcc tcgcccgctt cgtcgagccg gaccgtccct cgaacccgga ccctgccatc
      901 cgcctccacg acgatcggcc cgcgggcgcg cccttcctcg atgtcgccga cgacctcctc
      961 gaacagatcc tcgatcgtca gcacgccggc cgtgccgccg tgctgatcca tcacgacggc
     1021 catctgcgcg cgcatccggc gcatggctgt cagcacttga tcgagctgcg ccggcccggg
     1081 aacgtagggc agcgccctcg cgtcggtggc ggccacggaa cggttcgaga tgaggtgcct
     1141 caggatctcc ttgatgtgca ggctgccgac gatgttgtcc aggtcgccgc tgtagacgag
     1201 gtaccgcgtg tgggcatgag cgcggacgat ggcgcgcagt tcgtctggct cggcgcctac
     1261 ggggatcccg gcgagcagca cgcggggcac catcacctca ccggccgtgc ggtcgccgaa
     1321 ctcgaagaga tcccggagaa tctcgcccga ctcgccgcgc agcagcccgc ctgcctggct
     1381 ctcacggacg atgaactcga gctcttcggc cgtgtggtag cgctcgcgat ccacttcacg
     1441 gcgctcgatg ccgaccagcc agagaacctt gttgccgatt tcgttcagcc cgacgatcag
     1501 cggcaggatc gcgtgctgca gggcccggat gatcggcgac acgtagagca ccgtcgtctg
     1561 cgcctgttga agcgcgagcg ccttcggcac catctcgccc agcacgatgt gcaggtaagt
     1621 gaggatggca actgccacga cgctcgcgac cgtgtgcgac agaatccacc ccgtgcgttc
     1681 cagagaggcg aaccagggct gcatctggtc tgccagccag tgctcgccgt acatcccgag
     1741 gccgaggctg gccaccgaga tgccgacctg cgtagtggcg atgtagcgat cctgcagctt
     1801 gggatcctcg aggatacgaa gcacacgcag cgccaggccg ctgccgagcg aggcctgatg
     1861 ctcgatgttc gagcggggcg cgccgacgat ggcgaactcg gccgcgacga acagcccgtt
     1921 ggcgagcacg agcaggagga tgatggcgat cggtatgacg atctccatca cgacgctccc
     1981 ccggatatca cgctcgccag cggcctgacc gcaagcgact gcggcgtgcg gtccactaca
     2041 cgctcgacct cgaactcgaa ctcgccgatc gcgatcgtct ccccggcgac gggaaggtgc
     2101 cccagtgcct ccgtgaccat tccgccgatc gtcgcggcgt cggtgtccca gacggcctgc
     2161 agcaccgacg cggcttcgac cacgctcatt tcgccgggaa ggcgcacgcg gccgtccgac
     2221 aacgtttccg cgccgggttc cgacttgaat tcgtcgccga catggccgat gagctccgtg
     2281 agcacatccg ccagcgtcag cagccccgcc gtgccgccga actcgtccac cacgagcgcc
     2341 tggtgcgacc ggcgctcgcg aagatccttg aggatgcgat ccgccgtcac gctctcgtga
     2401 acgctcggga ccgggcggat cagatcggcg agcttcgtgc cgggggcgcc ttccacgaac
     2461 catcgagcca gatccttggt gcgcaggatg cccacgatgt tgtcgatcga ttcgcggtac
     2521 accggcatcc gcgaatatgg gctctgcgtg acgatgctga cgacttcggc gagcggagtg
     2581 ttgacctcga tcgccgcgat gcggcgccgg ggcaccatga gctgcctggc ctggcgcagg
     2641 ttgagctgca gcgcgcgctg cagccgcctg tgctcgtcag gctcgagcag gccgccgtcg
     2701 cggctctccg cgatcaacag ctcgatctct tcgggcgagt ggatgtgccg atgggcctga
     2761 tgctgcgcac ccatcaggcg caggatcagg aggcccgtgc cgttcagcca ggtgatgaac
     2821 gggcgataca cccattgcga ggcgaccatc ggcacgagcg tgtagagaga cgtttgcgtc
     2881 gggtactgca gcgcgagcga cttcggcacc agttccgcga agaccgtctg cgccacggtc
     2941 agcacgagga ggacgacggc cgtcgaggtt gactgcgcga cgacatcctg aagaccgcca
     3001 aaacgggcga cgagcggcgc cagccagacg gcgatcgtcg cctgcgcata ggccccgaga
     3061 acgaggctcg acagcgtgat cccgatctgg cacgccgcga tgtagcgatc cagcgcggcc
     3121 ggggattcga tgagcggcag aagccacgcg gcaaagggat taccgccttc ggcgagctgg
     3181 tggagccggc tgcgcctcac gctgacggcg gcgaactccg ccccgacgta gagcgcgttg
     3241 acgaggatga gcgccgcgat gacggggatg gccgcaggga ccatttcgag gctcagtact
     3301 tagcaccctt tcgagggcaa ggggtaagcc gttgcagtgc agcagcttct ggcgccaaag
     3361 gtgtgaaggg tcagacccca tgatacgggt cgactcatgg ggtctgaccc caaggcggga
     3421 ataagaccga tttggtcggt gttccacaag gcagaggcac aggatggaca gaacccagga
     3481 tcgggccctt attgccgggc ttcagtcggg cagccacgag tcgctcaggc agatcgcgga
     3541 cctctatggc ccgcgcgtgt accagctcgc gatgcggcac atgaagaaca gggaggacgc
     3601 cgaggaagtc acccaggacg tgctgatgaa ggtctatcgc aaggccgggt cgttccgcgg
     3661 tgacgccgca ctctcgtcgt ggatctatcg cattacgttc aacaccgcga tgtcgcggct
     3721 gcgccagcgg tacagcgccc gggcggcgga gcagccgcgg gacaacgcgc gcttcgaggc
     3781 gtgggaccag ccttcacacg tgcagggcga gcctgccgac tggtcgcaca tgcccgacga
     3841 cgagctgctc cggctggaac tgcgccaggc cgttgccgcg gcaatccgcg atctgcccga
     3901 gatctatcgg cagccgatca tccttcggga cctcgagggg ttgacgacag aagaggcgag
     3961 cacgcgcctg aagctcaagg acgagacgct caagtcccga ctccatcggg gacggttgct
     4021 cctgcgcgag cggctgcgtg tcttcgccac cgggctcacg ctgcaccggc cgacgacggc
     4081 gtattcgtag tcggtcgtgt cacgcttgcc ggaccgtaga gttcggcgga ctgctcaacg
     4141 aggcatgccg agcgtgattc gcccgtagcc gttgaacatg aaccggcatc gctccgtttc
     4201 gcagacgaga ctggtcgtgt gcaagcgact cgttgcctcc cgcggccagt cgcacccgcg
     4261 agcgcaggag acggtcacct gcccgcgtgg aacctcgacg ttgatctcga aatcggcaac
     4321 ggctctctgc gcgccgaccg accacccggc ggccacgaga agagccgcaa ccacgacgag
     4381 ccgcgcgtga cgtctcatgc gtcgcctccc gcctacttcg atacgaactc cggcttcgtc
     4441 tcccgattcg ccaggctcgc cgccagctcc agcgccagcc gcgccactct cgtagttccg
     4501 ggcacgtcga tcttctgcca gtcgtcgccc ggccggtggt agtcgtcgtg gaatcccgtg
     4561 aagaagttga tggccggaat tcgccgcgcg atgaagctgt agtcgtcgct gcggcccgcg
     4621 cccgggccct cgcggcgaac tttcagcgtc gaaccgctcg ccttcacggc ggcctcgagg
     4681 tcggcctcca tcgacggcga ggcctccagg ccgctgacgt ccacggcgcc gttggcgcgg
     4741 ccgaccatgt cgaggttcag catcgccacg gtgttctcga tgggaacacg gggctggctc
     4801 acgtaatggg ccgatccgag gagcccgcgt tcttcaccgg caaaggcgac gaagatcagc
     4861 gtgcgtggaa aacgcgcgcg atcggcggca gcggcgcggg cgatctcgat gattgacgcg
     4921 gtgcctgacg cgttgtcgtc cgccccgttg tggatctcgc ccgtcttgtc cggcgactgc
     4981 gacagacggc cgccgaatcc gacgtgatcg tagtgtgcgc cgatgacgat ggcttcgcgc
     5041 gcgcgtgcgg cgtcgctgcc cggcagcacg ccgaccacgt tgcgaaccgt gtgcttgttc
     5101 ttggcgagat gctcgacgta gtccactcgt gcgtcgggca gatccctcga ccgcggcatg
     5161 aggtcggcat cgatctcgcg agcggtctcg tcgagccgcc acgcttccac cagcggctgc
     5221 gcttcagccc gccggattcg aagcaggggg atgccgtgat cctcggcgtc cggatcggcg
     5281 ccgaacaacc cgtacggcgc atcgtctacg cgatgagtgg ggtcgagtac gacgatcagc
     5341 gcctttgcgc ccctgactcg cgcggccgcc gccttggctg ccaccgtcgt ctgctgcatc
     5401 gggcgtgtgc cgttgagacg gctgttgggg tcgtgttcct ggggctcgtg gctgaggatc
     5461 aggacggctt tgccgttgac gtctatgcgc gcgtagtcgt cgtagatccc cggcacgacg
     5521 aggccgtagc cggcgaagac gaccggcagg ccgcgcaggt ccgctgacgg cttcgagacg
     5581 tcttcattgg atggggccga gagcgggtag tagctcgtcc cgaccacgaa gctggccttc
     5641 cggcctttgc cgctgatcga caaggtgttc acggacccga ccttgagccc ggcgacgagc
     5701 tcgaagggct ggaaccagtc gccgcccgcg ccgccgggtt gaagacccac ggccttgaac
     5761 tgcgccgcga cgtaatcgcc ggcagaatcg agcccgggtg ttccgttcgc gcgccccttc
     5821 aaatcgtcgg aggacaggaa ccggatgtgc tcgagcaggg caccggacgc gatgtcggc
//
LOCUS       DBNGSH010000285         4317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2399492, whole genome shotgun sequence.
ACCESSION   DBNGSH010000285 DBNGSH010000000
VERSION     DBNGSH010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2399492"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="AC16CY_06670"
     CDS             complement(<1..259)
                     /locus_tag="AC16CY_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR3353501.1"
                     /translation="MRSGFVSFIGRPNAGKSTLLNRIVGQKLAIVSDKPQTTRTRVVG
                     VKNYERGQVVFVDTPGVHKPAHRMNVRMVDVALEAIREVDVL"
     gene            complement(269..1570)
                     /locus_tag="AC16CY_06675"
     CDS             complement(269..1570)
                     /locus_tag="AC16CY_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3353502.1"
                     /translation="MTPLALFLLGCATVFLGSVQVAFSALMRLSLRLMAERGGRDDRL
                     GFYLDDPLQLFIPVRMLIGVCTVLAGALIARLSNIQDALSIAVFIVSLVAFVLVCEHL
                     LPMLIVRQDPERVLDVLLPVFHYVARVLTPLTAAVLGVVGVRREAANGTSEAAPPLLA
                     AASEQGEGAISQQEGRELLQSIVDFTETVAREVMTPRPDIVAIRADATLLDLRTLFRE
                     EQYSRMPVYRDNLDNIVGIVFVKDLVALPPGTEPPLTTLMRSAYFVPESKRASELLKE
                     MQRRQAQMAIVVDEYGGTGGLVTVEDLLEEIVGEIRDEYDVESETVTDEGNGVFVFSG
                     KVSVDEVRDRLGVEIEREGFETVGGFLLSHLGRMPYVGEKVEVEDLAFEVLEVERRRV
                     TKLRVRRTDTEPRTERERERREPTERERETQHEPGTVKREV"
     gene            complement(1567..2049)
                     /gene="ybeY"
                     /locus_tag="AC16CY_06680"
     CDS             complement(1567..2049)
                     /gene="ybeY"
                     /locus_tag="AC16CY_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3353503.1"
                     /translation="MTARSSGTESSTRFPLRPATLVVEVVAAGRLRAPGLARWLQSVA
                     PPRVRGLVTVALVPDARVRRLNARYRGRDSATDVLSFSADEPGQFGDVVIACGVAKRQ
                     ARRAGHPVQVELRVLALHGLLHLLGYDHEHDDGRMAQVERRLRRKGGLSEGLIERERR
                     "
     gene            complement(2036..3010)
                     /locus_tag="AC16CY_06685"
     CDS             complement(2036..3010)
                     /locus_tag="AC16CY_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3353504.1"
                     /translation="MKRIPLPQEGLETLYGAHDGNLKHIESLLNVQIRTQGGELIVEG
                     DRTGEARVERIFNQLLGLMSEGYDLRNGDVRTAAQLVADDESVDLRDYFLKEGQRQPE
                     GTRRRVKPKSTNQRRYLDAIERFDIVFGVGPAGTGKTYLAMAQAVSYLLAKKVSRIIL
                     ARPAVEAGEKLGFLPGDLQEKVNPYLRPLYDALYDMMDTERATRLVERGTIEVAPIAF
                     MRGRTLNDAFVILDEAQNTTSEQMKMFLTRLGFGSKAVVTGDITQIDLPHPRTSGLVE
                     VLKIVKDIEGIGFIYFDERDVVRHRLVQQIVKAYDVFSGSGRNGNDRS"
     gene            complement(3083..>4317)
                     /gene="aspS"
                     /locus_tag="AC16CY_06690"
     CDS             complement(3083..>4317)
                     /gene="aspS"
                     /locus_tag="AC16CY_06690"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR3353505.1"
                     /translation="GARDYLVPSRVHAGEFFALPQSPQLFKQILMVSGMDRYVQICKC
                     FRDEDLRADRQPEFTQVDVEMSFARPETIFGVIEPLMRDIFAVIGRQIETPFPRMPYT
                     EAMAKYGSDKPDLRCGMPIQDVSTLFRESSFEVFRKIVAGGGAVRAFVVPNAAGYSRS
                     AVDGIVGQEKAQGRHLLWARRAEDGTITSSFPKAVGEETVRRLLDASGTSNGQLLFVV
                     AGEADAASKALGQLRLTLARKDGLLDPEKFAFTWVVDFPLLEWDADDKRFVAMHHPFT
                     SPLDEDIPKLESDPGAARAKAYDLVLNGSEIGGGSIRIHDADMQRRMFSLLNIGAEEA
                     KARFGFFLEALEYGTPPHGGIALGLDRIAALLANESSIREVIAFPKTATAVDLMSDAP
                     SPVDDKQLRELHLQIRKT"
BASE COUNT          705 a         1514 c         1352 g          746 t
ORIGIN      
        1 ccaggacgtc aacctcgcgg atggcctcga gcgcgacgtc caccatgcgg acgttcatcc
       61 ggtgcgccgg cttgtggacg cccggcgtat ccacgaacac cacctggcca cgctcgtagt
      121 tcttgacacc caccacgcgt gtcctggtgg tctgcggctt gtccgagacg atggcgagct
      181 tctggccgac gatgcggttc agcagggtcg atttgccggc gttggggcgg ccgatgaacg
      241 agacgaagcc ggatctcatc accccggttc acacttctcg cttcacggtt cccggttcgt
      301 gttgagtttc acgttcgcgt tcagtgggtt cacgacgttc acgttcacgc tcggttctgg
      361 gttcggtgtc agtacggcgc acccgcagct tcgtgacccg gcggcgctcc acttcgagga
      421 cctcgaaggc caggtcctcg acctccacct tctcgcccac gtacggcatc cggccgagat
      481 gcgacaggag gaaccccccg acggtctcga agccctcacg ctcgatctcc acgcccagcc
      541 gatcccgtac ttcgtctacg ctgaccttgc cgctgaacac gaagacgccg ttaccctcgt
      601 cggtcactgt ctcgctctcg acgtcgtact cgtcgcgaat ctcgccgacg atctcctcga
      661 gcaggtcctc caccgtcacc aggccgccgg tgccgccgta ctcgtccacg acgatcgcca
      721 tctgcgcctg ccggcgctgc atctccttca gcagctcgga tgcccgcttg ctctcgggga
      781 cgaaataggc cgagcgcatc agcgtcgtca gcggcggctc ggttcccggc ggcagggcaa
      841 cgagatcctt gacgaacacg atgccgacga tgttgtccag gttgtcgcgg tacacgggca
      901 tgcgggagta ctgctcctcg cggaacagcg ttcggaggtc cagcagcgtc gcgtcggccc
      961 gtatcgcgac gatgtcgggc cgcggcgtca tgacctcgcg cgcgacggtc tccgtgaagt
     1021 cgacgatcga ctggagcagc tcgcgcccct cctgctgcga gatcgcgccc tctccctgtt
     1081 cgctggcggc ggcgagcaat ggcggcgccg cctcggatgt gccgttggcc gcctcacggc
     1141 ggacgccgac cacgccgaga acagccgccg tcagcggcgt cagtacgcgc gccacatagt
     1201 ggaagacggg cagcaggacg tcgagaacgc gttccggatc ctggcgcacg atgagcatcg
     1261 gcagcaggtg ctcgcagacc aggacgaagg ccacgagcga cacgatgaac acggcgatcg
     1321 acagggcgtc ctgaatgttg ctgaggcgtg cgatcagggc gcccgccagc accgtgcaca
     1381 cgccgatcag catgcgcacc gggatgaaga gctggagcgg atcgtcgaga tagaagccca
     1441 ggcggtcgtc gcggccgccg cgctccgcca tcagccggag cgacaaccgc atgagggcgc
     1501 tgaaggcgac ctggacggat cccaggaata ccgtcgcgca cccgagcagg aagagcgcca
     1561 gcggagtcat cgacgctccc gttcgatcaa cccctcgctc aagccgccct tccggcggag
     1621 ccgccgctcc acctgggcca tccggccgtc atcgtgctcg tgatcatagc caagaaggtg
     1681 caggaggcca tgcagcgcga gcacccgcag ctcgacctgc accggatgac ccgccctgcg
     1741 cgcctgacgc ttcgccactc cgcaggcgat gaccacgtcg ccgaactgcc ccggctcatc
     1801 cgcggaaaac gagagcacat ccgtggccga gtcacggccg cggtatcgcg cgttgagccg
     1861 gcggacccgc gcgtccggga cgagcgccac cgtcaccaga ccccggacgc gcggcggcgc
     1921 caccgactgg agccagcggg ccagccctgg cgctcgcaga cggccggcgg cgacgacctc
     1981 gaccacgagc gtcgccggcc ggaggggaaa tcgtgtggag gattccgtcc cgctgctacg
     2041 agcggtcatt cccattgcgc ccgctgccgg agaagacgtc gtacgccttc acgatctgct
     2101 gcacaagccg atggcgcacc acgtcacgct cgtcgaaata gatgaagccg atgccctcga
     2161 tgtccttgac gatcttgagc acctcgacca gcccggacgt gcgcgggtgc ggcaggtcga
     2221 tctgcgtgat gtcgcccgtc accaccgcct tcgagccgaa cccgagccgg gtaaggaaca
     2281 tcttcatctg ctcggacgtc gtgttctggg cctcgtccag gatcacgaac gcgtcgttca
     2341 gcgtccggcc gcgcatgaag gctatgggcg caacttcgat cgtcccgcgc tcgaccaggc
     2401 gcgtcgcgcg ctcggtgtcc atcatgtcgt agagggcgtc gtagagcggg cgcagatacg
     2461 ggttcacctt ttcctgcagg tcgcccggca ggaagccgag cttctcgccc gcctcgacgg
     2521 ccgggcgcgc gaggatgatg cggctgactt tcttcgccag caggtagctg acggcctggg
     2581 ccatcgcgag atacgtcttc cccgtacccg ccggtccgac gccgaacacg atgtcgaacc
     2641 gctcgatggc atccaggtat ctgcgctggt tggtgctctt cggcttgacc cgcctgcgcg
     2701 tcccctcggg ctgccgctga ccttccttca ggaagtagtc tcgaaggtca acgctctcgt
     2761 cgtcggccac cagttgagcc gcggtccgca cgtccccgtt ccgcaggtcg tacccctctg
     2821 acatcaggcc gagcagttga ttgaagatgc gctcgacgcg cgcctccccg gtgcggtcgc
     2881 cctcgacaat cagctcgccg ccctgcgtgc ggatctggac gttgagcagc gactcgatgt
     2941 gcttcaggtt tccgtcgtgc gctccataca gcgtctcgag accttcctgg ggcagcggaa
     3001 tcctcttcat tacacttcgt tctccggtct tcgatctctg gggcctgggg ccctgaggcc
     3061 tgggccctga ggccttctcg atctacgtct tcctgatctg caggtgcagt tctcgcagct
     3121 gcttgtcgtc caccggcgat ggcgcgtcgg acatgaggtc cacggccgtc gccgttttcg
     3181 ggaaagcgat gacctcgcga atcgagctct cgttggcgag aagcgccgca atacgatcga
     3241 ggccgagcgc gatgccgccg tggggcggcg ttccgtactc gagcgcctcc aggaagaacc
     3301 cgaaccgggc cttcgcctcc tccgcgccga tgttgaggag ggagaacatg cgccgttgca
     3361 tgtctgcatc gtggatacgg atgctcccgc caccgatctc gctgccgttc agcaccaggt
     3421 cgtacgcctt ggcgcgggcc gcacccgggt ccgactcgag cttggggatg tcttcgtcga
     3481 gcggagaagt gaacgggtgg tgcatcgcca cgaaacgctt gtcgtccgcg tcccactcga
     3541 gcagaggaaa gtcgacgacc cacgtgaacg cgaacttctc cgggtcgagc aacccgtcct
     3601 tcctggcgag cgtcaaacgc agctgcccca gggcctttga tgcggcgtca gcctcccccg
     3661 ccacgacgaa caggagctgt ccgttgctcg tgccgctcgc gtcgagcagc cgccgtaccg
     3721 tctcctcgcc gaccgccttc gggaacgacg aggtgatcgt gccgtcttcc gcacgccgcg
     3781 cccacaggag atgccgcccc tgtgccttct cctggccaac gatgccgtcg acggcgctgc
     3841 gcgaatagcc cgccgcgttc ggcacgacga aggctcggac cgcgccccct ccggccacaa
     3901 tcttccgaaa cacctcgaag gacgactcgc ggaacagtgt gctgacgtcc tgaatcggca
     3961 tcccgcaccg aaggtccggc ttgtcggagc cgtacttcgc catcgcttcc gtgtagggca
     4021 tgcggggaaa cggcgtctcg atctgcctgc cgatcaccgc gaagatgtcc cgcatgagcg
     4081 gctcgatgac gccgaagatc gtctccggcc tggcgaatga catctcgacg tccacttgcg
     4141 tgaactcggg ttgcctgtcg gcgcgcaggt cctcgtcccg gaagcactta cagatctgca
     4201 cgtagcgatc catccccgag accatcagga tctgcttgaa gagctgcggc gactgcggca
     4261 gcgcgaagaa ctcgcccgca tggacgcggc tcggcaccag gtagtcgcgc gcccctt
//
LOCUS       DBNGSH010000286        13590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2060171, whole genome shotgun sequence.
ACCESSION   DBNGSH010000286 DBNGSH010000000
VERSION     DBNGSH010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 13590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13590
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2060171"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1966
                     /locus_tag="AC16CY_06695"
     CDS             131..1966
                     /locus_tag="AC16CY_06695"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphohydrolase"
                     /protein_id="HIR3353506.1"
                     /translation="MPQQSYWLASLALVWGVFYFGLRAIRVLRKRFNAERKRAQQMSE
                     LHLATIEALARAIDAKDGMAENHIRRVQLFATAVARELGMTEPEVQAVRTAALLHDIG
                     KLAVPDHILAKPGALTSEEFQKVKTHPQVGAEIIASVPFPYPVAPLIQNHHERWDGRG
                     YPRGLKGEEIPLGARVLFLVDYFDALTSTRPYHRPMTYEAALALIQQEAGKALDPRAV
                     DVFVRIFPRVRADAERAERELSGTHSGPVEGAAQAPAAEGKATVFDDIADAHGEIYAL
                     YQIAQTMGTSLGVSDTMALIASKLSNLVPFSACALFLYDESTDSLTCRFATGTDFELM
                     KQLRLPAGQGVTGWVARNRRSLVNTAPGADLEAGGLSLPTVLASALVCPLTFRDRVIG
                     TLAVYHTERSFYRDDHRRLLDRVCEQAAAVVHNAIVFEQTQAASLTDPLTGLPNTRFM
                     ANHLARELSRAERGSTEVSLIVMDLDDFKDVNDGYGHQVGDRALREVATVLRGVIRPY
                     DVCVRYAGDEFIVVLAGCGWDEAESKRLELQAAVERLPFEARPGEQMPLAISAGTAVF
                     PIDGVTYESLLAKADSRMYRNKVLHKRDARQRRNAESAPDGRAAS"
     gene            complement(2042..2812)
                     /locus_tag="AC16CY_06700"
     CDS             complement(2042..2812)
                     /locus_tag="AC16CY_06700"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR3353507.1"
                     /translation="MADFTQKHGLIVGVANKRSISWAIAQAVARRGARLALTYQGRFD
                     EHVHELSQGLEQPLVLPCDVSSDTEIAAVFEKVAQEFGGLDFLVHGAAFAPREELEQP
                     FVMTSRDGFRVTMDVSAYSLIALTRHAMPLFESRGGGAVLTLSYLGSERVFPNYNVMG
                     VAKAALEATVRYLAADVGPKNVRVNAVSAGPIKTLAAAGIKGFSNILHVYPERAPLRR
                     NVEASEVAEAAAFLLSDAGKGITGEVLLVDSGFHVTGM"
     gene            complement(2854..3582)
                     /locus_tag="AC16CY_06705"
     CDS             complement(2854..3582)
                     /locus_tag="AC16CY_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353508.1"
                     /translation="MQHRRVRLGDVLDDYCPRERRITNHAVVAMIEDEVKRTRCTTCD
                     AEHEYKEAKAPPPRQKKKPVVPAAVAPRSADPPALVASEPEEAIEADNAVEPVESAEP
                     PPGPEDADVVSETAGDADDEWPVHRPLIRATLPRPEGQVPERKAPDFTIRQGGRFDEE
                     RNGNRARGKRPSRHGGQGSSGQSRFGSPQSRSGQGHGSRHGGQHSGNPHRHGNRPPGS
                     GSGRPHGGRHGGGGGQGRGPKRGR"
     gene            complement(3622..5019)
                     /gene="argH"
                     /locus_tag="AC16CY_06710"
     CDS             complement(3622..5019)
                     /gene="argH"
                     /locus_tag="AC16CY_06710"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3353509.1"
                     /translation="MTLWSGRFDRAPDPAAFEFGVSFGFDRVLFEDDVTGSIAWAQAL
                     GAAGVLSADDVAAILGGLQEILDEGRRNPAWISGGDEDVHSFVERQLVERIGDAGRRL
                     HTGRSRNEQVSLDLRLYLRRRIPLLQRGVARLVAACLDRAAGAGSAIMPSYTHLRRAQ
                     PVLVAHFFLSHAAALRRDHARLAAAGSEADALTLGSGAIAGTSYAVDTGLLSRALGFS
                     RVVSNSMDASSDRDFVASFLYAAALGMVHLSRLSEDLIIFTGEEHGFFDLADESATGS
                     SMMPQKKNPDPLELVRGKSGRAIGRLTGWLATMKGLPSGYNKDLQEDKEAVFDAEAAY
                     AASLGAAAAVVSRLSINADRAASAASGLLLATDVADYLVARGMPFRRAHEVVGAMVRR
                     LLAEGRDFSSLTMAEWREASEMFGDDAPRAATALASVQARRTPQSTNPSAVRNALEEC
                     QQWVVSVLHSHEPSP"
     gene            complement(5016..5918)
                     /locus_tag="AC16CY_06715"
     CDS             complement(5016..5918)
                     /locus_tag="AC16CY_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HIR3353510.1"
                     /translation="MTPIVLAYSGGVSSSMAVRWLADTYATDIVTVSLDVGQREDLGE
                     LRRRALSCGARRAHVVDVRDELARAAFTGPLLRGTLGDGGCPRIDQWPLPLIARALLE
                     IARIEGARSVAHGSSDHALDAAIAAIDPSAAVLAPSRGIAPADRTSVGYRIDENLWGR
                     LVSWPRGDEAPSIVRPAATTSTEHARLDIGVESGVPVSINGVPMSPAELVESLTLIAG
                     RHGVGRFESSVDGRQVVCDAPAAVVLRTALAAAGESGVARLAVLNGTCALAPSHGSAF
                     DFAQAGTDMPSSPRDPEVELVNQA"
     gene            complement(5915..6367)
                     /locus_tag="AC16CY_06720"
     CDS             complement(5915..6367)
                     /locus_tag="AC16CY_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0034618 - arginine binding [Evidence IEA];
                     GO_process: GO:1900079 - regulation of arginine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR3353511.1"
                     /translation="MKARRQSAVLDVIRREPVRSQEQLRRMIRASGFDVTQATLSRDI
                     RELGLVKGGPHGAYQTPVAAEPNGHTSKSMLQRALASQLRRIDRVQQLVLLKTGLGHA
                     QSLCAALDGAGLPDVVGTLAGEDTILIVAPDARRARTLVKRLEEMSHL"
     gene            6500..7537
                     /gene="argC"
                     /locus_tag="AC16CY_06725"
     CDS             6500..7537
                     /gene="argC"
                     /locus_tag="AC16CY_06725"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3353512.1"
                     /translation="MALTSPLATERSDAAPPQLTRVAIAGATGYAGQELVRILARHPR
                     VRLTAAMSSGATSAPRPLPSLARIWDGSIQPLDVDRLANDADVVFLALPEAASAELAP
                     VLLDRGARIIDLSGAFRIRDAAHRQRWYPATAALPQGTVYGMTEGRQTEIRRARLVSN
                     PGCYPTAALLALEPLHRAGLLTGPVIVDAKSGVSGAGKTPSERTHFSENHGSVAAYAV
                     LQHRHTAEIEQEIGAPVTFVPHLVPLDRGILETIYGHLSAGATESRVSEAFKAAYADA
                     PFVRLTGTAMPEIKHVAHTNFCDIGWVVDEERRRIVLVAVLDNLLKGAAGSAVQNLNV
                     MLGLDERTGLL"
     gene            7534..8349
                     /gene="argB"
                     /locus_tag="AC16CY_06730"
     CDS             7534..8349
                     /gene="argB"
                     /locus_tag="AC16CY_06730"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR3353513.1"
                     /translation="MTRRPRLVLKLGGELLEQSQDLGRVARAVANLAARASLVVVHGG
                     GREIDAALHAAGIPKQQVDGLRITDARTLDVVVSVLAGAINTRLVAAVRRAGARPVGL
                     TGADAAVVTVKRAAPIVSLAGDRVNLGLVGAPVNNGTPRLLTDLLAGGYVPIVACIGA
                     TRDGRLLNVNADTLASHLARALGATRLVIAGGTSGVLDEQGKTIDRLTMRDAARLVKA
                     GVANKGMVAKLQACRAALQGGVRDVVIADGRRTSLGTLADPSAPLSACTQVIR"
     gene            8346..9557
                     /locus_tag="AC16CY_06735"
     CDS             8346..9557
                     /locus_tag="AC16CY_06735"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3353514.1"
                     /translation="MTTFSDIQARESQFVLNTYRRQPVAFVRGKGPRLYDVDGRAYLD
                     FVSGIGVASLGHGHAGLAAAIADQAATLIHTSNLYYHPFQAEAASRLSALSGLPRSFF
                     CNSGTEANEACLKFARRYWYTQGVTDRTGFVAIEGAFAGRTMGALAITHDEHYRTPFG
                     PLVGPVTFVSADSPEALVAAVGDRTAAVIAEPIRGEGGIRPLPRAFSDAIAEVCRRTG
                     TLLIADEVQTGLGRTGYPFYFKALGWQPDLVSVGKALGSGVPVGAALVSERVAAAISP
                     GDHGSTYGGNLLATRAAVFFLEQLMDRGLLEHVRSVGDHLERRLRTIALKHPLIVEVR
                     GAGLMRGVELRVDATPVVDLARERGLLVNRTNERVVRLLPPLTIETADIDAGIEILDT
                     VLAEVPSEVHA"
     gene            9554..10129
                     /locus_tag="AC16CY_06740"
     CDS             9554..10129
                     /locus_tag="AC16CY_06740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3353515.1"
                     /translation="MSAVPVALAIRRAIVAPPVHEAVNVDYSIRAARESDADAIHRLI
                     SEHLAEGRLLPRSRDEIRSRVHRFIVASDEAGIVACADLAPLSRRVAEVRSLVVADEA
                     RSRGICRRLVQELERRALAAGFATLCAFTHAPGYFVKMGFSVVPHTWLPEKIEADCRA
                     CAQFRRCGQYAVMLPLVASPVARVPAESAHA"
     gene            10122..11360
                     /gene="argJ"
                     /locus_tag="AC16CY_06745"
     CDS             10122..11360
                     /gene="argJ"
                     /locus_tag="AC16CY_06745"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR3353516.1"
                     /translation="MPEAPAIRDVAMTPVPGGVTAPEGFRSAALHCGIKAAAGALDLA
                     IIAADASVPAAGIFTTNLAQAAPVLVSKRHLSASGGMARAIVVNSGCANACTGDRGMS
                     DAVQMAADVASALSCPAEHVLVASTGVIGVNLKMDKVSPGIASAAAALARGRGSEAAR
                     AIMTTDPFSKEHAITVQAAHGSFTVGGTAKGSGMIEPNMATMLAFLTTDARVPAPMLH
                     HALTESARDTFNAITVDGDGSTNDSLFAMASGASGLAIDEESYPALVAAFLAVSTDLA
                     LGIVRGGEGATKVIAVHVSDAASRDDARRVARTIANSPLVKTAVHGADPNWGRIVAAA
                     GRAGVKFDMGRATVRVGGVLLFDHGLPHDEAAPLAEEHMKGTDVRIDVSLGTGGRQAA
                     TIWTCDLSAEYVRINGAYRT"
     gene            11357..12310
                     /gene="argF"
                     /locus_tag="AC16CY_06750"
     CDS             11357..12310
                     /gene="argF"
                     /locus_tag="AC16CY_06750"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR3353517.1"
                     /translation="MRNSPAPAGFAKDFLSILDLSHDDLERLLRSSAQMKAERRLRRQ
                     APSAAALAGLHVALLFEKPSLRTRSTFEIAIRELGGDTLHLPAEFAEGVREPLEDIAR
                     NLERWVRALVIRTFGQDKAATLAAAGRRLHVINALTDEEHPCQALADAFTLRERWGDC
                     RGRTIAFVGDGNNVAASISHAACMMGVRVHVASPAGYALPDHVVAAASRVARHGAEVR
                     RFTDPRQAVTGVDAVYTDVWASMGEEAEAAERRRVFAPYQVNSELMAAAAPDALFMHC
                     LPAHRGEEVASEVIDSSASIVFDQAENRLHTQKALLSMLIG"
     gene            complement(12343..>13590)
                     /locus_tag="AC16CY_06755"
     CDS             complement(12343..>13590)
                     /locus_tag="AC16CY_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3353518.1"
                     /translation="VADVFFVGRLGPDAVATVGITESLMTLMYALAIGLSIGAGAVVS
                     RRIGEKDREGAARAAIQSILLGLFVAAIAAVLGATFGPRLLRVMGASDEVIRIGSDFA
                     RVMLGGSGTVLMLFLINAVFRGAGDAAIAMRVLWFANAINIVLGPCLVFGIGPFPELG
                     VTGAAVGTTIGRGSGVLYQLYQLAQRGGRIEIRPSHLRLDVGVMRSILRISGTATFQN
                     FVGMASWMGLVRILTGFGSAAVAGNTIGIRIILFALLPSFGVSNAAATLVGQNLGAGK
                     PDRAEAAAWRAGMYNTICLSIVGAVFLLFAPQLIQFFTDDPEVAGYGVRCLRIVSAGF
                     LFYGYAMVLTAAFNGAGDTRTPTLINIACLWLWEIPLAWALAYPLGLGPTGVFVAVSV
                     AFSTMAVVAGWLFSRGTWKTKRV"
BASE COUNT         2131 a         4766 c         4650 g         2043 t
ORIGIN      
        1 tcgacctcca ggatcagttc gctatcggag atgagattcc ttaaccacgg ttcttttttc
       61 gcgagagcaa acatgagagc ccgagttacg atcatctggc taccagtcaa cgtcctctag
      121 gagccccggg atgccccagc agtcgtactg gctagccagc ctggcgctag tctggggtgt
      181 gttctacttc ggactacgcg ctattcgtgt gctccgcaag cgattcaacg cggagcggaa
      241 gcgcgcccaa cagatgtccg agctgcacct tgcgacgatc gaggcgctcg cacgcgcgat
      301 cgacgccaag gacggcatgg ctgaaaacca catccggcgc gtgcaactct tcgcgacggc
      361 ggtcgcgcgc gagctcggca tgaccgaacc ggaggtccaa gccgtgcgaa ccgccgcgct
      421 actgcacgac atcggcaagc tcgccgtacc cgatcacatc ctcgccaagc cgggagcgct
      481 gacgtcggaa gagttccaga aggtcaagac gcatccgcag gtcggcgcgg agatcatcgc
      541 gtcggtgccc tttccatatc cggtggcgcc gctcatccag aatcatcacg agcgatggga
      601 cggccgcggc tatccgcgcg gtctgaaggg ggaggagatc ccgctcggcg cgcgggtcct
      661 gttcctggtg gactacttcg acgcgctcac gtccacgcgc ccctaccaca ggccgatgac
      721 ctacgaggcg gccctggccc tcatccagca ggaggcgggg aaggccctcg atccgcgggc
      781 ggtggacgtc ttcgttcgaa tctttccgcg ggtgcgggcc gatgccgaac gcgccgaacg
      841 cgagctgagt ggaacccatt ccgggccggt cgaaggcgcg gcgcaggcac cggccgcaga
      901 gggaaaggcg acggtgttcg acgacatcgc cgacgcgcac ggcgaaatct acgccctgta
      961 ccagatcgcg cagacgatgg gcaccagcct gggcgtgtcg gacaccatgg cgctcattgc
     1021 gtcgaagctg tcgaacctgg tgccgttctc cgcttgcgcc ctgttcctgt acgacgagag
     1081 caccgacagc ctcacctgcc ggttcgcgac gggcaccgat ttcgagctga tgaagcagtt
     1141 gaggctgccg gccggccaag gcgtcactgg ttgggtcgcc cggaacaggc ggtcgctcgt
     1201 caacacggcg cccggagcgg acctcgaggc cggcggcctg agtctgccca cggtcctcgc
     1261 atctgccctg gtatgcccgc tcacgttccg cgatcgcgtc atcggcacgc tggccgtcta
     1321 tcacaccgag cgcagtttct accgggacga tcaccgccgg ctgctcgacc gcgtgtgcga
     1381 gcaggctgcg gccgtcgtcc acaacgccat cgtcttcgag cagacgcagg cggcgtcgct
     1441 gaccgatccg ctcacgggcc ttccgaacac gcgcttcatg gccaatcatc tggcgcggga
     1501 gttgtcgcgc gccgagcgcg ggagcaccga ggtttcgctg atcgtcatgg acctcgacga
     1561 cttcaaggac gtcaacgacg gctacggaca tcaggtcggc gatcgcgcgc tgcgcgaggt
     1621 ggccacggtg cttcgcggcg tgattcgacc ctacgacgtg tgcgttcgat acgcgggtga
     1681 cgagttcatc gtcgttctcg ccggctgcgg gtgggacgaa gccgagtcca agcgactcga
     1741 gctgcaggcg gccgtggaac ggctgccatt cgaagcgagg ccgggcgagc agatgccgct
     1801 ggccatcagc gcgggcacgg cggtgttccc catcgacggc gtcacctacg aatcgctgct
     1861 ggccaaggcg gatagccgca tgtaccgcaa caaggtgctc cacaagcgcg acgcgcggca
     1921 gcgccggaac gcggagtcgg caccggacgg tcgagccgcg tcctgacggc cgaggtgcga
     1981 gggtgcgcct gcccgccgta gcgcgtagcg cgaaggtggg agggtgcgcg cgcttcgcgg
     2041 gctacatccc ggtcacgtgg aatcccgagt cgacgaggag cacctcgccc gtgatcccct
     2101 tgccggcgtc gctcaacaga aacgcggcgg cttcagcgac ttcggatgct tcgacgttgc
     2161 ggcgaagcgg cgcacgctcc gggtagacgt ggaggatatt ggagaacccc ttgatgcctg
     2221 ccgcggcgag cgtcttgatg ggacctgccg acaccgcgtt cacgcggacg tttttcggtc
     2281 cgacgtctgc ggcgaggtaa cgcaccgtgg cttcgagcgc ggccttcgcc acacccatga
     2341 cgttgtaatt gggaaacacc cgttcgcttc cgaggtacga caacgtcagc accgcgccgc
     2401 ccccgcgcga ttcgaagagc ggcatcgcgt gcctggtgag cgcgatgagc gagtacgccg
     2461 acacgtccat cgtgacgcgg aacccgtcgc ggctggtcat cacgaacggc tgctcgagct
     2521 cctcccgcgg cgcgaacgcc gcgccgtgca ccagaaaatc gaggccgccg aactcctggg
     2581 cgaccttctc gaacacggcg gcgatctccg tgtcggagga cacgtcgcac ggcagcacga
     2641 gcggctgctc gagcccctgc gagagctcgt ggacgtgctc gtcgaaccgt ccctgatagg
     2701 tcagcgcaag ccgcgcgccg cgcctggcga ccgcctgcgc gatcgcccac gaaatcgagc
     2761 gcttgttggc gacgccgacg atcagtccat gcttctgcgt gaaatcggcc atgaggttat
     2821 cgggctgccg gcggcgggct tcaggcgacg ggcctaacga ccgcgcttgg gtcctcgccc
     2881 ttgacctccg cctccgccgt gccggccgcc gtgcgggcgg cccgagcctg atccgggggg
     2941 gcgattgccg tgacgatgag ggttgccgga gtgctggccc ccgtggcggg aaccgtgccc
     3001 ctgccccgaa cgtgattgag gcgaaccgaa gcgggattga ccagacgatc cctgtccgcc
     3061 gtgccgtgac ggccgctttc cgcgagcacg gtttccattc cgttcctcgt cgaatcgccc
     3121 gccctgccgg atcgtgaaat ccggcgcctt gcgctcggga acctgtcctt cgggacgggg
     3181 cagcgtcgcg cggatgagcg gccggtgcac cggccactcg tcgtcggcgt caccggccgt
     3241 ctcgctcacg acgtcggcgt cctccggccc cggcggcggc tcggcagact cgaccggctc
     3301 gacagcgttg tcggcttcga tcgcctcctc gggctcggac gcgaccaggg cgggcgggtc
     3361 ggcggaccgg ggcgccacgg ccgctgggac gacaggcttc ttcttctgcc gcggcggcgg
     3421 agctttggct tccttgtact cgtgctcggc gtcacacgtg gtgcagcgtg tccgcttcac
     3481 ctcgtcttca atcatcgcga cgaccgcatg gttggtgatg cggcgctcgc gcggacagta
     3541 gtcatcgaga acgtcaccga gccgaacccg gcgatgctgc atgaactccc ccggagcaac
     3601 cttcaggtgg ggcgggtgaa atcagggtga aggttcgtgt gagtgtaaca cggacaccac
     3661 ccactgctga cattcctcca aggcgttgcg cacggccgac gggttggtcg actggggcgt
     3721 tcgccgcgcc tgcacggacg ccagcgcggt cgcggcgcgg ggtgcgtcgt ctccgaacat
     3781 ctcgctcgcc tcgcgccatt cggccatggt gagcgagctg aaatcacgcc cctcggccag
     3841 cagcctccgc accatggcgc ccaccacttc atgcgcccgg cgaaacggca tgccgcgggc
     3901 cacgaggtag tcggcgacat cggtggccag cagcaatccg gaggccgcgc tcgcggcgcg
     3961 gtcggcgttg atcgacagcc gggacacgac cgcggcggcg gcgccaagcg aggccgcgta
     4021 cgccgcttcg gcgtcgaaca ccgcttcctt gtcctcctgc agatccttgt tgtatccgct
     4081 cggcaaccct ttcatggtgg ccagccagcc tgtgagccgg ccgatggccc tccccgactt
     4141 cccccgcacg agctcgagcg ggtcggggtt cttcttctgc ggcatcatgc tgctgccggt
     4201 cgccgattcg tcagccaggt cgaagaagcc gtgctcctcg ccggtgaaga tgatcaggtc
     4261 ctccgagagc cggctcaagt gcaccatgcc caacgcggcc gcgtagagga acgacgccac
     4321 gaaatcacga tcggacgagg cgtccatgct gttggacacg acgcgcgaaa aaccgagcgc
     4381 ccgggacagc aggcccgtat cgacggcgta gctcgtaccg gcgatcgcgc ccgaaccgag
     4441 cgtcagcgcg tccgcttcgg agccggcggc cgcaagacgg gcgtgatcgc gccgcagcgc
     4501 cgcggcgtgg gacaggaaga agtgcgcgac cagcaccggc tgggcgcggc gcagatgcgt
     4561 ataggacggc atgatcgcgg agcccgcgcc tgccgcccga tcgaggcacg cagcgacgag
     4621 ccgggccacg ccgcgctgga ggagcgggat ccggcggcgc agatagagcc tgagatcgag
     4681 agagacctgt tcgttgcgcg atcgcccggt gtgcagacgg cgtcctgcgt cgccgattcg
     4741 ttccacgagc tgccgctcga caaagctgtg cacgtcctcg tcgccgccgc tgatccaggc
     4801 cgggttgcgg cgcccttcgt cgagaatctc ctggagtccg ccgaggatcg cggcgacgtc
     4861 gtcggccgac agcacgccgg ccgcgccaag cgcctgcgcc cacgcgatgc tgcccgtcac
     4921 gtcgtcctcg aagagcacgc ggtcgaagcc gaacgagacg ccgaactcga aggcggcggg
     4981 atcgggagcg cggtcgaagc gtccggacca gagcgtcacg cctgattcac cagttccact
     5041 tcgggatcac gtggagacga aggcatgtct gtcccggcct gagcgaagtc gaaggcggac
     5101 ccgtgcgagg gtgccagtgc acacgtgccg ttcaggacgg cgagacgggc cacgccgctc
     5161 tcgcccgctg ccgcgagcgc cgtgcgcagc acgacggcgg ccggcgcatc gcacacgacc
     5221 tggcgcccgt caacgctgga ttcgaaccgt ccgacgccgt ggcgcccggc gatgagcgtg
     5281 aggctttcga cgagctccgc cggcgacatc ggcacgccgt tgatcgacac cggcacgccg
     5341 gactcgacgc cgatgtcgag gcgggcgtgc tccgtggacg tcgtggcggc cggccggacg
     5401 atggaaggcg cctcatcccc gcgcggccag ctcacgagcc ggccccacaa gttctcgtcg
     5461 atccggtacc cgacactcgt tcgatctgcg ggcgcgatgc cgcgcgacgg cgccagtacg
     5521 gccgccgacg ggtcgatcgc cgcgatcgcc gcgtccagcg cgtggtcgga cgagccgtgt
     5581 gccaccgacc gcgcgccctc gatccgcgcg atctccagga gcgcgcgggc aatgagcgga
     5641 agcggccact ggtcgatccg cggacagccc ccgtcgccga gagtgccgcg caggaggggc
     5701 cctgtaaacg ccgcgcgcgc cagctcgtcg cgcacgtcca cgacgtgagc ccgccgcgcg
     5761 ccgcacgaca gcgccctccg ccgcagctcg cccaggtctt cccgctgacc cacgtcgagg
     5821 gagacggtga cgatgtcggt cgcgtaggtg tccgcgagcc agcggacggc catcgacgaa
     5881 ctgaccccgc ctgaataggc gagcacgatc ggcgtcatag gtgcgacatc tcctccaggc
     5941 gtttgaccag cgtgcgcgcg cgccgcgcat cgggcgcgac aatcaggatc gtgtcttccc
     6001 cggcaagagt cccgaccacg tcgggcagcc ccgccccgtc gagcgcggcg cacagcgact
     6061 gcgcgtgccc cagccccgtc ttcaggagca cgagctgctg gacgcggtcg atccgccgca
     6121 gctgcgatgc gagcgcgcgc tgcagcatgg acttcgacgt gtgcccgttc ggctcggccg
     6181 ccaccggcgt ctgatacgcg ccatggggac cgcccttcac gagaccgagc tcccggatgt
     6241 cccgcgacag ggtcgcctgc gtcacgtcga accccgaggc gcggatcatg cggcgcagct
     6301 gctcctggct gcgcaccggc tcacgccgga tgacgtcgag gacggccgac tgtcgtcgcg
     6361 ctttcattgg ctctcggctc ccggttcctg ggcctggctg aggcctgagg cctgggccct
     6421 gagccctgcc aagagcataa cgcaagactt gactgtttat acatacctct gtataaatat
     6481 tctttcggat cgagctatca tggcccttac gagccctctt gcgaccgaac gctccgacgc
     6541 cgcgccgccg cagctgacgc gcgtggcgat cgccggcgcc acgggatacg ccggacagga
     6601 actggtccgc atcctggccc gccatccgcg cgtgaggctg acggcggcca tgtcctccgg
     6661 cgcaaccagc gcgccgcggc cgctcccctc gctcgcgcgc atctgggatg gatccattca
     6721 gccgctcgac gtggatcgcc tggcgaacga cgccgacgtc gtgttcctgg cgcttcccga
     6781 ggcggcatcc gccgagcttg cgccggtgct cctcgaccgc ggcgcccgga tcatcgacct
     6841 ctccggcgcg ttccggatcc gcgacgccgc ccatcggcag cggtggtacc cggcgacggc
     6901 cgcgctgccc cagggcacgg tgtacggcat gaccgagggt cggcagacgg agatccgccg
     6961 cgcgcgtctc gtctcgaacc cgggctgcta tccgacggcg gcgctgctgg cgctcgagcc
     7021 gcttcatcgc gccggcctgc tcacgggtcc ggtgatcgtg gacgccaagt cgggcgtctc
     7081 gggcgcgggc aagacgccgt ccgagcggac gcacttctcc gagaaccatg gcagcgtcgc
     7141 ggcctacgcc gtgcttcagc accgtcatac ggcggagatc gagcaggaga tcggcgcgcc
     7201 cgtcaccttc gttccgcacc tggtcccgct cgaccgcgga atcctggaga cgatctacgg
     7261 tcacctgtcg gctggagcga ccgagtcccg cgtgtccgag gccttcaagg ccgcttacgc
     7321 ggacgcgcca ttcgtccggc tcaccggcac cgcgatgccc gagatcaagc acgtcgcgca
     7381 caccaacttc tgcgacatcg ggtgggttgt ggacgaggaa cgccggcgga tcgtcctggt
     7441 ggcggtgctc gacaacctgc tcaaaggcgc cgcgggctcc gccgtccaga atctcaacgt
     7501 catgctcggc ctcgacgagc ggacgggact cctgtgacca gacgtcctcg cctggtcctg
     7561 aagctgggcg gcgagctgct cgagcagtcg caggacctgg gccgcgtcgc gcgggccgtt
     7621 gccaacctcg ccgcgcgcgc ctcgctcgtg gtcgtccacg gcggcggccg cgagatcgac
     7681 gccgcgcttc atgccgcggg catacccaaa caacaggtgg acgggctgcg aatcaccgac
     7741 gcccgcacgc tcgacgtggt ggtttcggtc ctggcgggcg caatcaacac gcgtctggtc
     7801 gcggccgtgc gccgcgcggg agctcgaccc gtcggattga cgggcgccga cgcggccgtc
     7861 gtcaccgtca agcgggccgc gcccatcgtc agcctcgccg gcgacagggt caacctcggc
     7921 ctcgtgggtg ccccggtgaa caacggcacg ccgcgactgc ttaccgatct gctggcgggc
     7981 ggctacgtcc cgatcgtcgc gtgcatcggc gccacccgcg acggccgcct gctcaacgtg
     8041 aacgccgaca cgctcgcatc gcatctggcg cgagccctcg gcgcgacgcg actcgtgatc
     8101 gcgggcggga cgtccggcgt tctcgacgaa caggggaaga cgatcgatcg actgaccatg
     8161 cgggacgcgg ctcgcctggt gaaagccggc gtcgcgaaca agggtatggt ggccaagctc
     8221 caggcctgcc gcgccgcgct gcagggcggc gtccgcgacg tcgtcattgc cgacggcagg
     8281 cgaacttcac tcggtacgct cgccgatccg tccgcgcccc tgtcggcgtg cacacaggtg
     8341 atccgatgac aacgttcagc gacatccagg cccgggaatc gcagttcgtc ctcaacacct
     8401 accggcggca gccggtcgcg ttcgtccggg gcaagggccc gcgtctgtac gatgtggacg
     8461 gccgcgccta cctggacttc gtctcgggca tcggcgtggc gtcgcttgga catgggcacg
     8521 ccggtctcgc ggcggcgatc gccgatcagg ccgcgacgct gatccacacc tcgaacctgt
     8581 actaccatcc gttccaggcc gaggcggcgt cacgcttgtc ggcgctctcc ggcctgccgc
     8641 gatcgttctt ctgcaacagc ggcaccgagg caaacgaagc ctgcctcaag ttcgcgcggc
     8701 ggtactggta cacgcagggc gtcacggatc gcacggggtt cgtcgcgatc gaaggcgcgt
     8761 tcgcgggacg gacgatgggc gctctcgcga tcacgcacga cgagcattac cgcacgccct
     8821 tcgggccgct cgtcggaccg gtgaccttcg tgagcgccga ctcgccggag gcgctcgttg
     8881 cggccgtcgg cgaccggacg gccgcggtga ttgcagagcc gattcggggc gagggcggca
     8941 tccgtccgct gccgcgcgcg ttttcggatg cgatcgccga ggtgtgccgc cggaccggca
     9001 cgctcctcat tgccgacgaa gtccagacag ggcttggacg gaccggatac ccgttctact
     9061 tcaaggcgct cgggtggcag cccgacctcg tgtcggtcgg caaggcgctc gggtcgggcg
     9121 tgcccgtggg tgcggcgctc gtctccgagc gggtggccgc ggccatctcc cccggcgatc
     9181 acggcagcac gtacggagga aaccttctcg ccacgcgggc cgccgtgttc ttcctggaac
     9241 agctgatgga tcgtggcttg ctcgagcacg tgcgctcggt cggcgaccat ctcgagcggc
     9301 gcctccgcac gatcgcgctc aagcatcccc tgattgtgga ggtgcgcgga gcgggcctga
     9361 tgcgcggggt ggagctgcgc gtggacgcga cgccggtcgt cgacctggcc cgcgagcgcg
     9421 gactgctggt caaccgcacg aacgaacggg tcgtccggct gctgccgccg ctcacgatcg
     9481 agacggcaga catcgacgcc ggcatcgaga ttctcgacac cgtgctagca gaagttccct
     9541 ccgaggtcca cgcatgagcg cggtgcctgt ggctcttgcg atccggcggg ccatcgtcgc
     9601 gccgcccgtt cacgaggcgg tgaatgtcga ttactcgatc cgcgccgcgc gcgagtccga
     9661 cgccgacgcc atccatcggc tgatctcgga gcacctggcg gaaggccgcc tgctgccccg
     9721 gagccgggac gagatccgca gccgcgtgca ccgcttcatc gtcgcgtcgg acgaggcggg
     9781 catcgtggcc tgcgccgacc tcgcgcccct gagccgccgg gtcgccgaag tccgatcgct
     9841 cgtggtcgcc gacgaagcgc ggtcgcgcgg catctgccgt cgcctggtgc aggaactgga
     9901 gcgccgggcg ctggccgcgg ggttcgcgac gttgtgcgcg ttcacgcacg cgccgggcta
     9961 cttcgtcaaa atgggcttct ccgtcgtgcc gcacacatgg ctgcccgaga agatcgaggc
    10021 cgactgccgg gcatgcgcgc agttccgccg ctgcggccag tacgcggtca tgctgccgct
    10081 cgttgcctcg cccgtcgcgc gagtgccggc cgagtccgct catgcctgaa gcgccggcca
    10141 ttcgcgacgt cgccatgacg ccagtgccgg gcggtgtgac ggcgccggaa ggattccggt
    10201 cggccgcgtt gcactgcgga atcaaggcgg ccgcgggcgc tctcgacctg gcgatcatcg
    10261 ccgccgatgc gagcgttccg gcggccggca tcttcacgac gaacctcgcg caggcagcac
    10321 ctgtgctcgt ctcgaagcgc cacctctccg cgagcggcgg gatggccagg gcgattgtcg
    10381 tcaacagtgg atgcgccaac gcgtgcacgg gcgatcgtgg aatgtcggac gccgtccaga
    10441 tggctgccga cgtcgcctcg gcgctgtcgt gtccggcgga gcacgtcctg gtcgcctcga
    10501 cgggggtgat cggcgtcaac ctcaagatgg acaaggtatc gcccggaatc gcgagcgccg
    10561 cggcggcgct tgcgcgcggc aggggaagcg aggccgcgcg cgcgatcatg accaccgatc
    10621 ccttctcgaa ggaacacgcg atcaccgtcc aggcggccca cggatcgttc accgtggggg
    10681 gcacggcgaa gggctccggc atgatcgagc ccaacatggc cacgatgctg gcgttcctga
    10741 cgaccgacgc ccgcgtgccg gccccgatgc tgcaccacgc cctgaccgag tcggctcgcg
    10801 ataccttcaa cgccatcacg gtggacggcg atggctcgac caacgactcg ctcttcgcga
    10861 tggcctcggg cgcgagcggc ctggcgatcg acgaggagtc gtacccggct ctcgtcgcgg
    10921 cgttcctggc cgtctcgacg gaccttgcgc tcggcatcgt ccgcggcggc gagggcgcga
    10981 ccaaggtcat tgccgtccac gtgagcgatg ccgccagccg cgacgacgca cggcgcgtcg
    11041 cgaggacgat tgccaactcg ccgctggtga agaccgccgt tcacggcgcc gatcccaact
    11101 ggggccggat cgtcgcggcg gccgggagag ccggcgtgaa gttcgacatg gggcgcgcca
    11161 ccgtgcgggt tggcggcgtc ctcctcttcg atcacgggct gccgcatgac gaggccgccc
    11221 cgctcgcgga ggagcacatg aagggaacgg acgtccgcat cgacgtcagc ctcggaaccg
    11281 gggggcgtca ggcggccacg atctggacgt gcgacctgag cgccgagtac gtgcggatca
    11341 acggagccta tcgcacgtga ggaactcccc cgccccggcc ggcttcgcca aggacttcct
    11401 gtcgatcctc gacctgtcgc acgacgatct cgagcggctg ctgcggtcct cggcgcagat
    11461 gaaagcggag cgacgcctgc ggcggcaggc gccgtcggcc gccgctctgg ccggcctgca
    11521 cgtggcgctg ctcttcgaga agccgtctct cagaacacga tcgacattcg agatcgccat
    11581 ccgcgagctc ggcggggaca cgctgcacct gccggccgag ttcgccgagg gcgtccgcga
    11641 gcccctcgag gacatcgcgc gcaacctcga gcgctgggtg agggccctcg tgatcaggac
    11701 cttcggacag gacaaggcgg cgacactcgc cgccgctgga cggcgcctgc acgtgatcaa
    11761 cgcgcttacc gacgaggagc atccgtgcca ggcgcttgcc gacgcgttca cgctccgcga
    11821 gcgctggggt gactgccggg gcaggacgat cgccttcgtg ggcgacggta acaacgtcgc
    11881 cgcgtccatc tcgcacgcgg cgtgcatgat gggcgtgcgc gtgcacgtcg cctcgccggc
    11941 gggatatgca ctgcccgacc acgtcgtggc cgccgcgtct cgtgtggcca ggcatggcgc
    12001 cgaggtccgg cggttcaccg atccgcgtca ggcagtgacg ggcgtggacg ccgtgtacac
    12061 cgatgtgtgg gcgtcaatgg gcgaggaggc ggaagccgcc gagcgccgcc gcgtattcgc
    12121 gccctaccag gtgaacagcg agctgatggc agcggcggct ccggatgccc tgttcatgca
    12181 ttgccttcca gcccaccgag gcgaggaggt ggcgtcggag gtcatcgact catccgcctc
    12241 gatcgtgttc gatcaggccg agaaccggct gcacacccag aaggcgctgc tctccatgct
    12301 gatcggataa cggggccgcc cctcgaccaa gctcggggca ggtcacaccc gtttggtctt
    12361 ccacgtcccc ctggaaaaca gccaaccggc cacgaccgcc atcgtggaaa aggccacgct
    12421 gacggcgacg aacacgcccg tcggcccaag cccgagcgga taggcgagcg cccacgccag
    12481 agggatctcc cacagccaca ggcaggcaat gttgatgagc gtgggcgtgc gcgtgtcgcc
    12541 ggcgccgttg aaggccgccg tcaggaccat cgcgtagccg tagaacagga agcccgccga
    12601 cacgatgcgg aggcagcgaa caccgtagcc ggccacctcg gggtcgtccg tgaagaactg
    12661 aatgagctgg ggcgcgaaga gcagaaagac tgcgcccacg atgctcagac agatcgtgtt
    12721 gtacatgccg gctctccagg ccgcggcctc ggcccggtcc ggctttccgg ctccaaggtt
    12781 ctgcccgacg agcgtggccg ccgcattgct gacgccgaac gacggcagca gcgcgaacag
    12841 gatgatgcgg atgccgatgg tgttgcccgc cacggcggcg ctgccgaacc cggtcaggat
    12901 gcgaacgagc cccatccacg acgccatccc gacgaagttc tggaacgtgg ccgttcccga
    12961 gatgcgaagg atcgaccgca tcacgccgac gtcgagccgg aggtgtgagg ggcggatctc
    13021 gatccgcccg ccgcgctgcg ccagctggta cagctggtac agcacgccgc tgccgcgtcc
    13081 aatcgtcgtt cccacggccg cgcccgtgac gcccagctcg gggaacggcc cgatcccgaa
    13141 gacgagacac ggcccgagca cgatgttgat cgcattggcg aaccagagga cgcgcatggc
    13201 gatcgccgca tcccccgcgc cgcggaacac cgcgttgatg agaaagagca tcaggacggt
    13261 tccgcttccg cccagcatca cgcgcgcgaa atcggacccg atgcggatca cctcgtcgct
    13321 cgcgcccatg actcgaagaa gccgcggccc gaaggtcgcc ccgaggacgg cggcgatggc
    13381 ggcgacgaac agtccgagga gaatcgactg gatcgccgcc cgagcggcgc cctcccgatc
    13441 cttctcgcca atccggcgcg agacgacggc ccccgcgccg atcgagagcc cgatcgccag
    13501 cgcgtacatc agcgtcatca gcgactcggt gatgccgacg gtcgcgacgg cgtccgggcc
    13561 gagtcggccg acgaagaaca cgtcggcgac
//
LOCUS       DBNGSH010000287         1828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4098230, whole genome shotgun sequence.
ACCESSION   DBNGSH010000287 DBNGSH010000000
VERSION     DBNGSH010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4098230"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..668)
                     /locus_tag="AC16CY_06760"
     CDS             complement(39..668)
                     /locus_tag="AC16CY_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR3353519.1"
                     /translation="MLATSSATLVHTIASRRATLETARGVRIASVLFISVLTAAAAQV
                     SVPVPFTSVPLTLQPMVVLLGGLALGSRLGSASQILYLAAGIAGLPVFAASPLLPPGP
                     LRLLGPTGGYLMAYPLAAFVTGYLAERGLDRRYATSVIAMLAGLIVIYAAGATWLGLF
                     ARTVSGSAAVGLGAALATGVFPFVIPDLIKLAVAAGLVPALWRLVGRAR"
     gene            complement(715..1374)
                     /locus_tag="AC16CY_06765"
     CDS             complement(715..1374)
                     /locus_tag="AC16CY_06765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3353520.1"
                     /translation="MKGFVLISVVLLLNVEAPRLHAQDAPAAQRHKPLFAPTDLGLLE
                     APDRAAWQKPEQIMDALHIADGSKVADIGAGAGWFTVHLAHRIGPNGIVYAEDVQREM
                     LEAIRRRVGRLGLQNVQTRLGTESDPNLPAAALDAVAMVDAYQEIADPVTFLKNLARS
                     LKPNGLIGIVNYKPGRGGPGPPGNVRVDRAVVVADAKAAGLRIADEETLPYQYLIVLA
                     K"
     gene            complement(1371..>1828)
                     /gene="aspA"
                     /locus_tag="AC16CY_06770"
     CDS             complement(1371..>1828)
                     /gene="aspA"
                     /locus_tag="AC16CY_06770"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF021900.7"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR3353521.1"
                     /translation="SIMPGKVNPSVPEMVNQVCYQVVGCDTTVCMAAEAGQLELNVMM
                     PVIAWNALHASTILRNAMQALRTRTVDGIAADARRARELLDRSTAQATALSPYLGYEK
                     TAEIAKESVKTGKPIRDVVLERGLIEPAELDRILSVEAMTRPGVTGRKP"
BASE COUNT          304 a          631 c          604 g          289 t
ORIGIN      
        1 gccacgcggc ggggactcac gctggaaccc tcaggtcgtc aacgcgctcg tccaaccagg
       61 cgccagagcg cgggaaccag acccgccgcc acggcaagct tgatcagatc gggtatcacg
      121 aacggaaaca cgccggtcgc gagcgcggcg ccgaggccca cggccgcgct gccgctgacc
      181 gtgcgagcga acaggcccag ccaggtggcg ccagcggcgt agatcacgat caggcccgcc
      241 agcatggcaa tcaccgacgt cgcatatcgg cgatcgagtc ctcgctcggc gaggtatccg
      301 gtcacgaaag ccgccaatgg gtacgccatc aggtagccgc cggttggtcc gagcagcctg
      361 aggggccccg gaggcagcag cggggacgct gcgaagaccg gcagtcccgc gatgccagcc
      421 gccagataca ggatctgact ggccgagccc agccgcgatc cgagcgcgag cccacccagc
      481 aggacgacca tcggctgcag cgtcagcggg acgctcgtga acggcaccgg cacgctcact
      541 tgcgcggcgg ccgccgtcag caccgaaatg aacaggaccg atgcgatcct gacaccccga
      601 gccgtctcga gcgtcgcccg gcgcgaggct atcgtgtgca cgagcgtggc cgatgaggtc
      661 gcaagcatcg ccgcggattg tactccaccg gccgggggcg tccggccgcc gacgtcattt
      721 cgcgagcacg atgaggtact gatacggcag cgtctcttcg tccgcgatgc gcagcccggc
      781 ggccttcgcg tcggccacca cgacagccct gtccacgcgt acgttcccgg gagggcctgg
      841 tccgccgcgc cctggcttgt agttgacgat gccgatgagg ccgttcggct tgagcgaccg
      901 cgcgagattc ttcaggaacg tcacggggtc cgcgatctcc tgataggcgt ccaccatcgc
      961 gacggcatcg agcgccgcag ccgggagatt gggatcgctt tccgtcccga gacgcgtctg
     1021 cacgttctgg agtcccagcc ggcccactcg acgccggatc gcctcgagca tctcccgctg
     1081 cacgtcttcg gcgtagacga tgccgttcgg tccgatgcgg tgggcgagat ggaccgtgaa
     1141 ccatccggcg ccggccccga tgtcggcgac cttcgatccg tcggcgatgt gcagggcgtc
     1201 catgatctgt tccggcttct gccaggcggc gcggtcgggg gcttccagca gtccaaggtc
     1261 cgtcggcgca aaaaggggct tgtgccgttg cgccgcgggc gcgtcctggg cgtgaaggcg
     1321 gggcgcctcg acgttcagca gaagcacgac ggagatgagc acgaatcctt tcacggtttc
     1381 cgtcctgtga ctcccgggcg cgtcatggct tcgacagaca ggatacgatc gagctccgcc
     1441 ggttcgatca ggccgcgttc gagcaccacg tcgcgaatcg gcttgcccgt tttcaccgac
     1501 tccttcgcaa tctcggcggt cttctcgtag cccagatacg ggctcagggc cgtcgcctga
     1561 gccgtgcttc tatcgagcag ttcgcgcgcg cggcgcgcat ctgccgcgat gccgtccacg
     1621 gttctcgtcc gcagcgcctg catggcgttg cgaaggatcg tcgaagcgtg gagagcattc
     1681 cacgcgatca cgggcatcat cacgttcagc tcgagctggc cggcctcggc cgccatgcag
     1741 accgtcgtat cgcagcccac gacctggtaa cacacctggt tgaccatttc aggcaccgac
     1801 ggattgacct tgcccggcat gatcgacg
//
LOCUS       DBNGSH010000288         1778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_871677, whole genome shotgun sequence.
ACCESSION   DBNGSH010000288 DBNGSH010000000
VERSION     DBNGSH010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_871677"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC16CY_06775"
     CDS             <1..195
                     /locus_tag="AC16CY_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353522.1"
                     /translation="VIETSDVEVPAAMAGWRRDGRFLSGFSADDGDTLGRQLAGSGIA
                     VLESEPATLKEIFFDRMEQA"
     gene            192..896
                     /locus_tag="AC16CY_06780"
     CDS             192..896
                     /locus_tag="AC16CY_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353523.1"
                     /translation="MIWYRHWLEIRGILWLLVISVLLLAGPRADMLAFGAENPTRFGR
                     EMLQSPLARELGRDALLAWSAFAERIFTSVFLAGLMLAGNGLRGPAFTACRAAEYTLT
                     LPIARTTVIWTRLAASFIGALLVGVLVLSAHVIDLRLRGVDVPLVPMAQSFAFSVPAA
                     MAWTAFMGASVAFLRPLWALLSSLLGFTLTIPIRHVVTAFPSGGDVPWGGLTAFIALT
                     GFAVALMLRKGPDREF"
     gene            896..1540
                     /locus_tag="AC16CY_06785"
     CDS             896..1540
                     /locus_tag="AC16CY_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353524.1"
                     /translation="MFASLWYRHWLEMRGGLFVAVVAVALLSAAYPFFANGNDGRVPH
                     VWLSSAASWALGFFNWGNGVRSIALFQPNHPSIQFTLGLPVSRVRLILTRLAVAYAAV
                     VALFTVMFVSNTLALLLMGRAPAFGAMAGSSALAALLVAAIVTMFGMLGLVDENHFNW
                     VLPVAFFAAFFWAWPATTAFIGSQVVPWTGVATLAFVIGGALSASVVLAYKKDF"
     gene            1595..>1778
                     /locus_tag="AC16CY_06790"
     CDS             1595..>1778
                     /locus_tag="AC16CY_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3353525.1"
                     /translation="MKTSIRALIVLVMLSAGVADAQSLAGNWQGTLQAGKDLRIVIVI
                     SSADAGGLRAVLYSIDQ"
BASE COUNT          230 a          584 c          601 g          363 t
ORIGIN      
        1 gtcatcgaga cgagcgacgt ggaggtgccg gccgccatgg cgggctggcg tcgcgacggg
       61 cgcttcctgt ccgggttctc ggccgacgac ggcgacacgc tcgggcgtca gctggccgga
      121 tcgggaatcg ccgtgctcga gtccgagccg gcgacgctca aggagatctt cttcgaccgg
      181 atggagcagg catgatctgg taccgccact ggctcgagat ccgcgggatc ctgtggctgc
      241 tcgtgatcag cgtgctgctg cttgccggcc cccgggccga catgctggca ttcggggccg
      301 agaacccgac ccggttcgga cgcgagatgc tgcagagtcc cctggcccgt gaactcggcc
      361 gggacgcgct cctggcctgg tcagccttcg ccgagcgcat cttcaccagc gtgttcctcg
      421 ccgggctcat gctggcggga aacggactca gaggcccggc ctttacggcc tgtcgcgccg
      481 cggagtacac gctgactctg ccgatcgctc gcacgaccgt gatctggacg cgcctggcgg
      541 ccagtttcat cggcgccctg ctggtggggg tgctggtgct gtcggcgcat gtgatcgatc
      601 tgcggctgcg gggcgtcgac gtgccgcttg tgccgatggc ccagtcgttc gcgttcagcg
      661 tcccggcggc aatggcgtgg accgccttca tgggggcgtc ggtcgcgttc ctgcgtccgc
      721 tgtgggcgct gctgtcgtcg ctgctggggt ttacgctcac gatcccgatc cggcacgtgg
      781 tgaccgcgtt tccgtcggga ggcgatgtgc catggggggg cctgacggcg ttcatcgccc
      841 tgaccggatt tgccgtcgcg ctcatgctgc gcaaggggcc cgacagggag ttctgatgtt
      901 tgcctcactc tggtaccggc actggctcga gatgcgcggc ggcctgttcg tcgccgtcgt
      961 cgccgtcgcg ctgctctcgg cggcctatcc gttctttgcg aacggcaacg acggccgggt
     1021 gccgcacgtc tggctgtcgt cggcggcttc gtgggcgctc gggttcttca actgggggaa
     1081 cggcgttcgg agcatcgccc tctttcagcc caaccacccg tccatccagt tcacgctcgg
     1141 cttgccggtc tctcgcgtcc gcctcatcct gacgaggctc gccgtcgcct atgcggccgt
     1201 cgtcgcgctc ttcacggtca tgtttgtctc gaacacactc gcgctattgc tgatgggccg
     1261 tgcgccggcg ttcggtgcga tggccggatc cagcgcactc gctgcgctgc tcgtcgccgc
     1321 catcgtaacg atgttcggca tgctcggcct cgtcgacgag aaccacttca actgggtctt
     1381 gccggtcgcc tttttcgccg ccttcttctg ggcgtggccg gcgaccaccg ctttcatcgg
     1441 cagccaggtg gtgccctgga ccggagtcgc gacgctcgcg ttcgtcattg gtggcgcgct
     1501 gtcggccagc gtcgtccttg catacaagaa agacttctga accgtaccaa tgatcgggct
     1561 cgtagcccga agcctgacgt caaggagatt gcccatgaag acctcgatcc gcgcactcat
     1621 tgttctcgtc atgctgtcgg ccggcgtggc tgacgcccaa agcctcgcag gcaactggca
     1681 ggggacgctg caggcaggaa aggacctgcg aatcgtaatc gtgatttcca gtgcagatgc
     1741 aggcggcctg agagccgtgc tgtacagcat cgatcagg
//
LOCUS       DBNGSH010000289         5380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4098373, whole genome shotgun sequence.
ACCESSION   DBNGSH010000289 DBNGSH010000000
VERSION     DBNGSH010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4098373"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..871)
                     /locus_tag="AC16CY_06795"
     CDS             complement(2..871)
                     /locus_tag="AC16CY_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HIR3353526.1"
                     /translation="MRKELISALVSESNAPGIALAPAALVVARVEYPRLDPDPYIARL
                     DAMGDAARRAVAQHVEDTGDTSAHGGIKALNEYLFGQMRFTGNRDQYEDPRNSCLNEV
                     LERRTGIPLTLSIVYMEVARRAGLYADGVNFPGHFLVRCPEPAGNPLVIDPFHSGALL
                     SEHNCRLLLQRHVGSEVAFSRSLLAPATRTQILVRMLLNLKRIYVHMRSFPQARDVTE
                     LLLAITPSALSELRDRGLLAYHMNDVSAALRDLQAYLKLASMSETDKETRAEHQQIWE
                     HVKTLRRRVAALN"
     gene            complement(879..1568)
                     /locus_tag="AC16CY_06800"
     CDS             complement(879..1568)
                     /locus_tag="AC16CY_06800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3353527.1"
                     /translation="MTRALQAIVLDFDGVIANSEPLHLMAFQHALAAEGVELTPDEYY
                     TRYLGYDDVGMFEALGRDRRVPMGEGRVATLVARKGERMQEMLRSGSVLFPGAVDFIR
                     EAASQVPLAIASGALRHEIEEIVDAAGVGGLFAAIVAAGDTPESKPSPAPYRLAFQSL
                     CEHSGRQLDPRRSVAIEDSRWGLQSARGAGLRLVGVTTSYTAAELTDAELVVDGLGAL
                     TLSALDRLCAS"
     gene            complement(1565..2218)
                     /locus_tag="AC16CY_06805"
     CDS             complement(1565..2218)
                     /locus_tag="AC16CY_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HIR3353528.1"
                     /translation="MVLGSHISGSIGVAALVLTTACSSGPPAPDDSSYLSTIAAARAT
                     TNRAFAELPECGSPSSPDECSPVPAAKRTELLPLRYYDVDPGLNVPAALRLSEDRPVF
                     DMPTSTGTLRKMQRVGVLEFRLGGEPMTLAAFVPNGTRRIESLFVPFADLTTGNETYP
                     AGRYLDLDPTATGIYAIDFNRAYNPYCAYNNTYECPYPPPSNRLKVAIRAGEKAPGA"
     gene            complement(2246..4021)
                     /locus_tag="AC16CY_06810"
     CDS             complement(2246..4021)
                     /locus_tag="AC16CY_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3353529.1"
                     /translation="MTTDLSLRKGGSWLLEDTPPSEPFTPEKLTDEQRLMGQTASEFV
                     SAEVMPALDRMEQKDWPLARALIRRCGELGLLGVAAPEAYGGLDLDKVSSIAISDRVG
                     PSASFATTFGGQTNLCILPLVLFGTPAQKEKYLPRLVAGEIVGAYALSESASGSDALA
                     AKTRAVRNGDGSWLLNGEKMWISNGGFADLFIVFAKVDGEHFTAFIVERAFGGLSSGK
                     EEHKMGLHGSSTTPILLQDVRVPAENVLGEVGRGHKVALNTLNYGRLSLGAMCTGGAR
                     AAIGDAASYAAARRQFGQPIASFGAIRHKLGEMIARTYACESLVYRTAGLIDGALGPP
                     PYEGAAIAQALEEFAVEASIAKVACSEALDFVLDENVQIHGGNGFVRDYNAERYYRDA
                     RVNRIFEGTNEINRLLIPGMLVRRAIKGEIPLIAAARKLQDEILSPSAGSAAVDDGAL
                     AEQTRAVTSFKKVALMIVGTAMQTYGEKLTDQQEVLGYAADILIDNFAAESAVLRARC
                     AAAAPSRATELHVAAAQVFVNDAAQRVENAARSALAGMAEGDTLRTLLAALRRVLKVT
                     PVNTVALRRRLADATVAERKYLLGW"
     gene            complement(4018..5196)
                     /locus_tag="AC16CY_06815"
     CDS             complement(4018..5196)
                     /locus_tag="AC16CY_06815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3353530.1"
                     /translation="MQDAVVVAATRTAVGKAPNGSLRTVRPDDMAAAVIEEALRRVPS
                     LRPADVDDVVMGCAMPEAEQGLNVARIASLRAGVPVEASAVTLNRFCASGLQAIASGA
                     EHIMCGFADVVIAGGTESMSLVPMGGNKVSPNPTLVEEYPDVYLSTGLVAENHAREHA
                     ISREEQDQFALRSHQRALAAIDAGRFADEITPLTYNVAEPSTNGKPGTRRVTFAQDEG
                     PRRDTSGAALAKLRPAFHAAGTVTAGNSSQTSDGAAAVVITSARAARDRGLEPLARLV
                     SFATAGVEPERFGIGPVPAVKKALALAGLTLDQIDLVELNEAFAAQVLACLRELPIDP
                     ERLNVNGGAIALGHPLGCTGAKLTTTLLHEMRRRKARYGLVTMCVGGGMGAAGIFERS
                     "
     gene            complement(5181..>5380)
                     /locus_tag="AC16CY_06820"
     CDS             complement(5181..>5380)
                     /locus_tag="AC16CY_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353531.1"
                     /translation="KAKFIIHGERDELIPMKAVREFYARLQEPKELVEIDRANHLFDG
                     QASEVGDAIEDLLSDYSCKTL"
BASE COUNT          792 a         1877 c         1847 g          864 t
ORIGIN      
        1 ctcagttgag cgccgcgacg cgccgccgga gcgtcttgac gtgctcccag atctgctggt
       61 gctccgcgcg cgtttctttg tccgtctcgc tcatgctggc cagcttcagg tacgcctgaa
      121 ggtcgcgcag cgccgcgctg acgtcgttca tgtgataggc gagcaggccg cggtcgcgga
      181 gctcgctcag cgccgacggc gtgatggcga gcagcagctc cgtcacgtcg cgcgcctggg
      241 gaaacgagcg catgtgcacg tagatgcgct tgagattgag cagcatgcgc acgaggatct
      301 gcgtccgcgt ggcgggagcc agcagcgatc ggctgaacgc tacctcgctg cccacgtgcc
      361 tctgcagcag caggcggcag ttgtgctccg acagcagcgc gcccgagtgg aacgggtcga
      421 tcaccagcgg gttgcccgcg ggctccggac agcgcacgag gaagtgtccc gggaagttga
      481 cgccgtccgc gtacaagccc gcccgccggg ccacctccat gtagacgatc gacagcgtca
      541 gcggaatgcc ggtgcggcgc tcgaggacct cgttgagaca gctgttgcgc gggtcctcgt
      601 actgatcgcg attgccggtg aaccgcatct ggccgaagag gtattcgttg agcgccttga
      661 tcccgccgtg cgccgacgtg tcgcccgtgt cctccacgtg ctgcgcgacg gcgcgacggg
      721 cggcgtcccc catggcatcg agccgcgcga tatacggatc cgggtcgaga cgcgggtact
      781 cgacgcgtgc cacgaccagg gccgccggcg cgagcgcgat cccgggggcg ttggattcgg
      841 agacgagcgc gctgatcagt tcttttctca cgcagtgttc agctcgcaca tagacgatcc
      901 agcgccgaca gcgtcagggc gcccaggccg tcaaccacca gctccgcgtc cgtcagttcc
      961 gcggccgtgt aactcgtcgt gacgccgacc agccgcaggc ccgcgccgcg cgccgactgc
     1021 agcccccatc gcgagtcctc gatcgccacg gagcggcgag ggtcgagctg gcggcccgag
     1081 tgctcgcaca gcgactgaaa ggcgagccgg tagggagccg gcgacggctt gctctcgggc
     1141 gtgtccccgg ccgcgacgat cgcggcgaac agaccgccca caccggccgc gtcgacgatc
     1201 tcttcgatct cgtgccgcag cgcgcccgag gcgatcgcga gaggcacctg agacgccgcc
     1261 tcgcggatga aatcgaccgc gccgggaaag agcaccgacc cggaccggag catctcctgc
     1321 atccgctcgc ccttgcgcgc gacgagcgtc gccactctgc cctcgcccat cggcacgcgg
     1381 cggtcccgcc ccagcgcctc gaacatcccc acgtcgtcgt agccgaggta gcgggtgtag
     1441 tactcgtcgg gcgtcagctc cacgccttca gcggcgagcg cgtgctggaa cgccatcagg
     1501 tggagcggct cgctgttggc gatcacaccg tcgaagtcca agacgatggc ctggagagcg
     1561 cgagtcatgc gcccggcgcc ttctctccag ctcggatggc caccttcaac cggttcgacg
     1621 ggggcggata cgggcactcg taggtgttgt tgtaggcgca gtacgggttg tacgcacgat
     1681 tgaagtcgat cgcgtagatg ccggtcgccg tcgggtcgag gtcgaggtac ctccccgccg
     1741 ggtaggtttc gttgcccgtg gtcaggtcgg caaacgggac gaagagcgac tcgatgcgcc
     1801 gcgtcccgtt gggcacgaac gcggccagcg tcatcggctc gccgccgagc ctgaactcga
     1861 gcacgcccac gcgctgcatc ttgcgcagcg tgccggtcga ggtcggcatg tcgaacacgg
     1921 ggcggtcctc cgacagacgc agtgcggcag ggacgttgag tccaggatcg acgtcgtagt
     1981 agcgaagcgg gagcaactcg gtgcgcttcg ccgccggaac cggcgagcac tcgtcggggg
     2041 acgaggggct gccgcactcg gggagctcgg cgaacgcgcg gttcgtggtc gcccgcgcgg
     2101 cggcgattgt ggacagatag gaggagtcgt ccggggcagg cggcccggac gagcaggcgg
     2161 tcgtcaacac caacgccgca acgccgatcg atccggagat atgtgagccc agaaccatga
     2221 accgctaccg gctaccagcg accggctacc agcctagtaa gtattttcgc tcagccaccg
     2281 tcgcatcggc cagcctgcgc cgcagtgcca ccgtgttcac cggcgtgact ttcagtacac
     2341 ggcgaagggc cgccagcagc gtgcgcagcg tgtcgccctc ggccatgccc gccagcgcgc
     2401 tccgggccgc gttctcgacc cgctgcgccg cgtcgttgac gaatacctgg gcggcggcca
     2461 cgtgcagctc ggtcgcgcgc gacggcgccg ccgctgcgca ccgggcgcgc aggaccgcgc
     2521 tctcggccgc gaagttgtcg atgagaatgt cggccgcgta acccagtacc tcctgctggt
     2581 ccgtgagctt ctcgccgtag gtctgcatgg ccgtcccgac gatcatcagc gccactttct
     2641 tgaagctcgt caccgcccgc gtctgttccg cgagcgcacc gtcgtccacg gcggcggatc
     2701 cggccgacgg cgacaggatc tcgtcctgga gcttccgcgc cgccgcaatc agcggaatct
     2761 cgcccttgat cgctcgccgg acgagcatcc cggggatcaa cagccggttg atctcgttgg
     2821 tgccctcgaa gatccggttc acgcgcgcgt cgcggtaata gcgctcggcg ttgtagtcgc
     2881 gcacgaaccc gttgccgccg tgaatctgca cgttctcgtc gagcacgaag tcgagggcct
     2941 cgctgcacgc gaccttggcg atcgacgcct cgacggcgaa ctcctcgagt gcctgcgcga
     3001 tcgcggcgcc ctcgtacggc ggcggcccga gcgcgccgtc gatgagcccc gcggtccggt
     3061 agacgaggct ctcgcacgcg taggtccggg cgatcatctc gcccagcttg tgccggatcg
     3121 cgccgaagga ggcgatcggc tgtccgaact gccgccgcgc ggcggcgtag gacgcggcgt
     3181 ctccgatcgc ggcgcgcgcg ccgcccgtgc acatggctcc caggctgagg cgtccgtagt
     3241 tcagcgtgtt gagcgcgacc ttgtggcccc gcccgacctc gccgagcacg ttctcggccg
     3301 gcacgcgaac gtcctggagc aggatcggcg tggtggacga gccgtgcagg cccatcttgt
     3361 gctcttcctt gcccgagctg agcccgccga acgcccgctc gacgatgaac gccgtgaagt
     3421 gctcgccgtc caccttggcg aagacgatga acaggtcggc gaagccgccg ttgctgatcc
     3481 acatcttctc gccgttgagc agccagcttc cgtcgccgtt cctgaccgcc ctcgttttcg
     3541 ccgcgagcgc gtcggacccc gaggccgact cgctcaacgc gtacgcgccg acgatctcgc
     3601 cggcgacgag cctcgggagg tacttctcct tctgggccgg cgtgccgaac agcacgagcg
     3661 gcaggatgca gaggttcgtc tgaccgccga atgtcgtggc aaaggatgcc gacggcccga
     3721 cgcggtcgct gatcgcaatc gaggacacct tgtcgagatc gaggccgccg taggcttccg
     3781 gcgcggcaac cccgaggagt ccgagctctc cgcagcggcg gatcaacgcg cgcgcgagcg
     3841 gccagtcctt ctgctccatg cggtcgagcg ccggcatcac ctcggcgctc acgaactcgc
     3901 tggcggtctg ccccatgagc cgctgctcgt cggtcagctt ctccggcgtg aacggctccg
     3961 agggcggcgt gtcctcgagc agccaggagc cgcccttgcg gagagaaagg tctgtcgtca
     4021 tgaccgttcg aagattcccg cggcgcccat gccgccgccg acgcacatgg tgacgagtcc
     4081 gtaccgcgcc ttgcgccggc gcatctcgtg gagcagcgtg gtcgtgagct tcgcgccggt
     4141 gcacccgaga ggatgcccga gcgcgatggc gcctccgttg acgttgaggc gctccgggtc
     4201 gatcggcagc tcgcgcaggc aggccagcac ctgcgcggcg aacgcctcgt tcagctccac
     4261 gagatcgatt tgatccagcg tcagtcccgc cagggccagc gccttcttca ccgcggggac
     4321 cgggccgatc ccgaagcgct ccggctcgac gcccgccgtg gcgaacgaca cgagcctggc
     4381 cagcggctcg agccccctgt cccgtgccgc ccgtgccgag gtgatcacga cggcggcggc
     4441 gccgtcgctc gtctgtgagg agttgccggc cgtgacggtg cccgctgcgt gaaacgcggg
     4501 gcggagcttc gcgagcgccg ccccggacgt gtcgcggcgc gggccttcgt cttgcgcgaa
     4561 cgtgacgcgg cgcgtgcccg gcttcccgtt cgttgagggc tcggccacgt tgtaggtcag
     4621 cggcgtgatc tcgtcggcga agcggccggc atcgatcgcc gcgagcgcgc gctggtggct
     4681 gcgcagcgcg aactgatcct gctcctcgcg cgagatggcg tgctcgcggg cgtgattctc
     4741 ggcgacgagc ccggtgctga gatagacgtc cgggtactcc tcgacgagcg tcgggttcgg
     4801 gctcaccttg ttgccgccca tcggcacgag gctcatcgac tcggtgccgc cggcaatcac
     4861 gacgtccgcg aacccgcaca tgatgtgctc ggcgcccgag gcgatcgcct ggagcccaga
     4921 cgcgcagaac ctgttgagcg tgacggcgga tgcctcgacc ggcacgcccg cgcgcaggct
     4981 cgcgatgcgg gcgacgttca acccttgttc cgcctccggc atcgcgcacc ccatcacgac
     5041 gtcgtccacg tcggccgggc gcagcgacgg tacgcggcgc agcgcttctt cgattacggc
     5101 cgccgccatg tcgtcggggc ggacggtcct gagcgagccg ttcggcgcct tgccgacggc
     5161 ggtgcgggtg gcggcaacga ctacagcgtc ttgcatgagt agtccgacag caaatcttcg
     5221 atcgcgtcgc ccacctcgct ggcctgaccg tcgaagaggt ggttggcgcg gtcgatctcg
     5281 acgagctcct tcggctcctg gagcctggcg tagaattcgc gcacggcctt catggggatc
     5341 agctcgtcac gttccccgtg gatgatgaac tttgccttgg
//
LOCUS       DBNGSH010000290         3273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2060529, whole genome shotgun sequence.
ACCESSION   DBNGSH010000290 DBNGSH010000000
VERSION     DBNGSH010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2060529"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16CY_06825"
     CDS             <1..188
                     /locus_tag="AC16CY_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353532.1"
                     /translation="NGGEPLHPVLTLIDERTYQPKGRVDTAERDSAGAYARHISQSVP
                     APDGVDLRHFLETPRAA"
     gene            complement(670..3114)
                     /locus_tag="AC16CY_06830"
     CDS             complement(670..3114)
                     /locus_tag="AC16CY_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353533.1"
                     /translation="MSLLQDVRFAVRLLLKDRWFTVMAAVVLALGIGANNAVFTIVNG
                     VLIRSLPFPEPDQLMMVLTRDNRGRDSGVSLKDFEDWRSSARTFSDLSFVFSGSFNVG
                     EEGRITEQYPCSYVSANFFRMLNASPQAGRDFTPEEDKPGSQPAVMISSNLWTQRYGR
                     DPATVGRTLRLNGVTATIVGVMAEGLRFPNEADVWLPAGSLPPAVIQTPRQARGYFSI
                     GRLADGVTVEQARAELKAIGTKLASDFPDTNKDLAPYAEPFAQRIVGPQLRLLFWSLL
                     GAVGFVLLVACSNVANLLLSRAAHRSAEVSVRVAMGASRWDIVRQLLVEAVLLSFVAG
                     AIGLVLSIGGIRWFDAETQNIGKPYWMVFTMDWRTFGFFLGVCLSTGVIFGLAPALHV
                     SRTNVFETLKEGGRSGSGGLRAKRWSAGLIVVQLALTLVLLAGAGFMMRSFLTMYGTD
                     IGIDTSRMLTMNMILPARKYPGQEDRAAFLRRLDERFATLDGIEAASSATTPPFTGGD
                     GRQIEVDGRAKGSGDRSPVVTMLSIGSRYFDTLGIRPVQGRIFVDSDGDPGTRSVVVN
                     QRLVAVHLAGENPIGKQIRLTQDAQGGDQTPWLTVIGVVPNVRQRNNNQEREPDPIAY
                     LPHILNNTMSRGTTVIARSRTVNTALAAQALREAMRVLDPDLALFNPRTMDETLAQQR
                     FLLRLFTVMFGTFAVIALVLAAVGLYAVTSYAVTQYTRDIGVRMVLGAQPGQVIWLFL
                     RRAFVQLGIGLAIGLAGAFGVGQLLQAFLFQTSTRDPVTLVSIAVLLIVVSLVACLWP
                     ARHATRLDPLVALRHE"
BASE COUNT          582 a         1087 c         1099 g          505 t
ORIGIN      
        1 caaacggagg cgagccgctg cacccggtgc tgaccctgat cgacgagcgc acctaccagc
       61 cgaaagggcg cgtggacacg gccgagcgcg actcggccgg cgcctatgcc cggcacatca
      121 gccagagtgt gcccgcaccc gacggcgtcg acctgcgcca cttcctggaa acgccaaggg
      181 ccgcgtagtc cggaagctcg catcgttcat cacatttcgt cggcgcactc gcggctcgga
      241 ccagcggtat gccgggtgcc gggcgcggat gctgcggccg ggaaatcggt cctcgctcgt
      301 accctgctgc tgcccgcgcg tctacctgct gatccgaggc gacatccgtt gcgcggctca
      361 aaacgttcgg tctgactcgt cgccactcga acgcgctggc ggcagacgcg cgggatgaat
      421 cgctccgccc gatttccgtt gcggtcgacg tcggcctggc atacgcgcac cgttacgcgg
      481 cataccgctg gtccgaaccg agcgtccaac ccgcgtcggc ccggggcccc cgccgcgcgt
      541 ggtttgcgcg gtggggtgga actcgcggga ttgccgacgt tccacataca agtgagctcc
      601 gctgactagg ttgccgcgag agccagacta cagaagctca cgttcagaac atcgttgatt
      661 ggacggcttt cattcgtgtc gcaacgccac gagcggatcg agacgcgtgg cgtgccgcgc
      721 cggccacagg caggcgacga gcgacaccac gatgagcagc acggcaatcg agacgagcgt
      781 caccgggtcg cgggtgctgg tttgaaagag gaaggcctga agcagctgcc cgacgccgaa
      841 agcgcccgcc aggccgatcg ccaacccgat gcctaactgg acaaaggcac gcctcaggaa
      901 cagccagatg acctgccccg gctgcgcgcc gagcaccatg cgcacgccga tgtcccgcgt
      961 gtactgcgtg acggcatacg acgtcacggc gtagagaccg acggccgcca gcaccagcgc
     1021 gatcaccgcg aaggtcccga acatcaccgt aaacaggcgc agcaggaagc gctgctgcgc
     1081 cagcgtctca tccatcgtcc tcgggttgaa gagcgcgagg tccggatcga ggacgcgcat
     1141 ggcctcgcgc agcgcctgcg ccgccagcgc ggtgttgacg gtgcgcgacc gcgcgatgac
     1201 tgtggtgccc cggctcatcg tgttgttcag gatgtgcggg aggtaggcga tgggatcggg
     1261 ctcccgctcc tggttgttgt tgcgctgccg gacgttgggc accacgccaa tcacagtgag
     1321 ccacggcgtc tggtcgccgc cctgcgcgtc ctgcgtcagc cgtatctgct tgccgatcgg
     1381 attctcgccg gcgaggtgca ccgcgacgag gcgctggttc acgacaacgc tccgcgttcc
     1441 cgggtctccg tcgctatcga cgaaaatgcg gccctgcacc ggacggatgc caagggtgtc
     1501 gaagtatcgg gatccgatgc tcagcatggt caccaccggt gaccgatcgc cggaaccctt
     1561 ggcccggccg tccacttcaa tctgccgtcc gtcgccgccg gtgaagggag gcgtggtcgc
     1621 gctggacgcc gcctcgattc cgtccagcgt cgcgaaccgc tcgtcgagcc gtcgaaggaa
     1681 ggcggcgcga tcctcctggc ccgggtactt tcgggccggc aggatcatgt tcatggtcag
     1741 catccgcgac gtgtcgatgc cgatgtcggt cccgtacatg gtgaggaagc tgcgcatcat
     1801 gaagccggcg cccgccagca gcacgagcgt cagcgccaac tggacgacga tgaggcccgc
     1861 cgaccagcgc ttggctcgca agccgccgga cccggaccgc cctccctctt tcagcgtttc
     1921 gaagacattg gtcctggaga catgcaatgc cggggcaagg ccgaaaatga cgccggtgga
     1981 gagacacacc cccagaaaga agccgaacgt gcgccagtcc atcgtgaaga ccatccagta
     2041 gggcttcccg atgttctgcg tctcggcgtc gaaccagcgg atgccgccga tggagaggac
     2101 gaggccgatc gctcccgcca cgaatgacag caggacggct tcgaccagca gttgacgcac
     2161 gatgtcccac cggctggcgc ccatcgccac gcgcacgctg acctcggcgg agcggtgagc
     2221 cgctcgcgac agcagcaggt tcgcgacgtt cgagcaggcc acgaggagga cgaagccgac
     2281 ggcgccgagc agcgaccaga acagcagccg aagctgcggg ccgacgatgc gctgggcgaa
     2341 cggctcggcg tacggcgcca ggtccttgtt ggtgtcggga aagtcggacg ccagcttcgt
     2401 gccgatggct ttcagctccg cgcgggcctg ctcgacggtc acgccatctg cgaggcggcc
     2461 gatcgagaag tatcctcgcg cctgccgcgg cgtctgaatg accgcaggcg gcaggctccc
     2521 ggccggcagc cagacgtcgg cttcattcgg gaagcggagc ccctcggcca tgaccccgac
     2581 gatggtcgcc gtcacgccgt tcagcctgag ggttcgaccg accgttgccg ggtcgcggcc
     2641 gtaacgctgc gtccagaggt tactgctgat catgacggcc ggctggctcc cgggcttgtc
     2701 ctcctcgggt gtgaagtcac ggccagcctg cggactggcg ttgagcatgc ggaagaagtt
     2761 ggccgacacg tagctgcagg ggtactgctc tgtaatccgg ccttcttccc cgacattgaa
     2821 cgagccgctg aacacgaacg acaggtcgga gaacgtgcgg gcggaggacc gccagtcctc
     2881 gaagtccttc agggagacgc ccgagtccct cccgcggttg tcgcgggtga gcaccatcat
     2941 gagctggtcg ggctcgggaa acggcaggct gcgaatcagg accccgttga cgatggtgaa
     3001 gacggcgttg ttggcgccga tgccgagcgc gagcaccacg gcggccatca ctgtgaacca
     3061 gcggtccttg agcagcagac gaacggcgaa gcggacgtcc tggagcagac tcatggcgga
     3121 ccctctgaag gctgaaggga cggatgaacc cttagacgtc gcgaacgaac aaaaggatcg
     3181 aggtgggctc tagctgccga gtgccttcgc gatcgccgcg cgcagcgccg gatcctcggg
     3241 cgtcaccttg ctctcgaaga aggcggtcac ctt
//
LOCUS       DBNGSH010000291         2768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_192683, whole genome shotgun sequence.
ACCESSION   DBNGSH010000291 DBNGSH010000000
VERSION     DBNGSH010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_192683"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..992)
                     /locus_tag="AC16CY_06835"
     CDS             complement(72..992)
                     /locus_tag="AC16CY_06835"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3353534.1"
                     /translation="MRIFLTGATGYVGSAILEASLRAGHGVTALVRDPEKAEHVTLRG
                     AQPVIGDLSKPAGYVAAAEECDVIIHAAFDRSKHGVEIDRLAIETLLGVAARRIDARE
                     KASVLYTSGVWVLGDTTGPAAEDAPLRPTPLVSWRPEHEGLVWRMSRGGRIRTAIVRP
                     GIVYGGARGIISDLLKDARNGLVRVIGDGKNHWPCIYDRDLADLFLRLAVHPSASGIF
                     HANDEADEHVGEIVDAIAAHAMVQQAVRHVPLEEARSKMGPYADALALDQIVRSPRAR
                     ALGWTPSLHSVAGNVARLLEEFRSAREAAA"
     gene            complement(1170..2537)
                     /locus_tag="AC16CY_06840"
     CDS             complement(1170..2537)
                     /locus_tag="AC16CY_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353535.1"
                     /translation="MTSPSSTSVGAQYDSLLQSALRQFFERASLDTEPSASASPDARL
                     SIEPSANPAALIVRWFGTRYTLRVPGRWMFTSHEVRLAQAIGAVLAARVSAIHDPRII
                     AERGDLFRGAIEDRYVGAFLDPRSYAIEAREARADRVASVIELLRVAALSSYENRPIS
                     TGVLLLGTLEDPCRPGAAFPASSPAYTESLTAIKSFHRLADGVRTVFLANAEGRLLDI
                     IDIERWGNQACRNQTVQVPCAKVFQPHARATLQHGHICAVLSPSREIKLFAEGAELFT
                     FRGASWHLLDLQAKYRLWAEAVGDEALSMRLFQTALDLADAREGALFAVARDVAGAVS
                     KLVAPGDRLDRSLASTRETPAAPSRRDLLHLLEGRSITDLDPNVLAALASLDGAIVVD
                     TSGRLLAAGAILRHPATAESEAGGVVEGARTTAALAASRYGPVLKVSEDGVITFFDGE
                     RVWDI"
     gene            2565..>2768
                     /locus_tag="AC16CY_06845"
     CDS             2565..>2768
                     /locus_tag="AC16CY_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353536.1"
                     /translation="MRRDTLIDFFRDLVEITGDFLVYDDGYRRRVHGYADVGRAARGF
                     AARLGESGIGKGDKVVFWGENRPE"
BASE COUNT          457 a          963 c          925 g          423 t
ORIGIN      
        1 ttcgctgttc tgggttcggc gttcagtcgt gtgtggaaca catgactgag cgccgctgac
       61 tgcgctgcga gtcaggctgc tgcttcgcgc gccgagcgga actcctcgag cagccgggcg
      121 acgttgcccg ccaccgagtg cagcgacggc gtccagccga gcgcccgcgc ccgtgggctg
      181 cggacgatct gatccagcgc cagggcgtcg gcgtagggcc ccatcttgga ccgcgcctcc
      241 tcaagcggga cgtgccggac cgcctgctgg accatcgcat gggccgcgat ggcatcgacg
      301 atctccccga cgtgctcgtc ggcttcgtcg ttggcgtgga agatgccgga cgcgctcggg
      361 tgcacagcaa gccggaggaa caggtcggcc agatccctgt cgtagatgca cggccagtgg
      421 tttttcccgt cgccgatcac ccgcacgagg ccgtttcggg cgtccttgag caggtcgctg
      481 atgatgcccc tggccccgcc atagacgatc cccggcctga cgatggccgt ccgaatacgg
      541 ccgccgcgcg acatccgcca cacgagcccc tcgtgctcgg ggcgccacga caccaggggc
      601 gtcggcctga ggggagcgtc ctccgccgcg ggtccagtgg tgtcgccgag cacccagact
      661 ccggacgtgt acagcacgct tgccttctcg cgcgcatcga tgcgacgggc cgccacgccg
      721 agcagcgtct ctatcgcgag tcgatcgatc tcgacgccgt gcttcgaccg atcgaacgcg
      781 gcgtggatga tgacgtcgca ttcctccgcc gccgcgacat acccggccgg cttggagaga
      841 tcgccgataa cgggctgcgc gccgcgaagc gtgacgtgtt cagctttttc cggatcgcga
      901 acgagcgccg tcacgccgtg tcccgcacga agcgatgcct cgagaatcgc ggacccgacg
      961 tatccggtcg cgccggtcag aaagatgcgc atggggggaa accattctag ctgctacctg
     1021 ctgccggctg ccagcagcaa gctaccagcc accagctaca ccacagctcg cttgtgttgg
     1081 tgggctccgg ccgaggcccc gctgatcaaa cagcctgccc tgcaagccac gatcgttggc
     1141 aacatcacgg acacaagcga gttccggggt tagatatccc agacccgctc gccgtcgaaa
     1201 aacgtgatca cgccgtcctc gctcaccttg agcaccgggc cgtaccgact ggccgcaagc
     1261 gcggccgtag tacgcgcccc ctcgacgacg cctccggctt cggactcggc cgtcgccgga
     1321 tgtcgaagga tcgcgccggc cgcgagcagg cgcccgctcg tatcgacgac gatcgcgccg
     1381 tcaagcgacg ccagcgcggc caggacgttg ggatcgaggt cggtgatcga tcggccttcc
     1441 agcaggtgca gcaggtcgcg gcgcgatggc gctgccggcg tctctcgcgt gctggccaga
     1501 gatcggtcga gacgatcgcc cggcgccacc agcttcgaca cggcgccggc cacgtcacgg
     1561 gcgacggcga acagggcgcc ttcgcgagcg tccgccaggt cgagcgcggt ctgaaagagc
     1621 cgcatcgaca acgcctcgtc tcccacggcc tcggcccaga gccggtactt ggcctgcaga
     1681 tcgagcaggt gccaggacgc gccgcggaac gtgaacagct cggcgccctc ggcgaagagc
     1741 ttgatctcgc gcgacgggct cagcacggca cagatgtgtc cgtgctggag cgtggccctg
     1801 gcgtgcggct gaaacacctt cgcgcacggc acctgcaccg tctgattccg gcaggcctgg
     1861 ttgccccacc gctcgatgtc gatgatgtcg agcaggcggc cttcggcgtt ggccaggaac
     1921 accgtccgca cgccgtccgc aagccggtgg aagctcttga ttgccgtcag tgactccgta
     1981 tacgccggcg aagacgccgg gaatgccgcg cctggccggc acggatcctc cagggtgcca
     2041 agcagcagga cgccggtcga gataggcctg ttctcgtagc tcgacagcgc cgccacccgg
     2101 agcagctcga tgacggaggc cacgcgatcg gccctcgcct ctcgagcctc gatcgcgtac
     2161 gagcgcggat ccagaaacgc accgacgtag cggtcctcga tcgcaccgcg gaacaggtct
     2221 cctcgctcgg cgatgatcct cgggtcgtga attgcgctga cgcgcgcggc cagcaccgcg
     2281 ccaatcgcct gagcgagcct gacttcgtgc gaggtgaaca tccaccggcc cggcacccgc
     2341 agcgtgtagc gcgtcccgaa ccagcggacg atcagcgcgg ccgggttcgc ggaaggctcg
     2401 atcgacaggc gcgcgtccgg ggacgccgag gcactcggct cggtatcgag gctcgcccgc
     2461 tcgaagaact gacgcagcgc cgactgcagc aggctgtcgt actgcgcacc gacgctcgtc
     2521 gaggaaggag aggtcatgga cgctttccgg atcataattc acgcgtgcgc cgcgacacgc
     2581 tcatcgactt ctttcgggac ctggtcgaga tcacgggcga cttcctcgtc tacgacgacg
     2641 gctaccggcg ccgcgtgcac ggctacgcgg acgtcggacg cgcggcgcgc ggctttgcgg
     2701 ccaggctcgg cgaatccgga atcggcaaag gcgacaaggt cgtgttctgg ggagagaaca
     2761 ggccggaa
//
LOCUS       DBNGSH010000292         3418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3079899, whole genome shotgun sequence.
ACCESSION   DBNGSH010000292 DBNGSH010000000
VERSION     DBNGSH010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3079899"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC16CY_06850"
     CDS             <1..213
                     /locus_tag="AC16CY_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3353537.1"
                     /translation="CFMFRAPMRMSALAFGTTIVLSTFALGVHWIPDMIAGFALGIAS
                     LLLAWRIVRQGRAPFLAGERLAVDAL"
     gene            complement(343..645)
                     /locus_tag="AC16CY_06855"
     CDS             complement(343..645)
                     /locus_tag="AC16CY_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353538.1"
                     /translation="MRRLLVVATSLAVLAAGGAFAGEVKGPPGTINNTNETGALDHAQ
                     SACAASGLNDFDQHEGQNVSQVQTAADAFKYYGLEPGVQGRGIPEFGVPGLCKGNN"
     gene            complement(775..1506)
                     /locus_tag="AC16CY_06860"
     CDS             complement(775..1506)
                     /locus_tag="AC16CY_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3353539.1"
                     /translation="MQKYRQYCPVARASEILADRWTPLIVRELVLGSHRFNEIERGLP
                     GISRSLLSSRLRDLEDSGVVERLPRVQSTMTEYHLSEGGRELKAVIEALGAWGVRWAF
                     GDPRPEELDAGLLVWKIHQRINRELLPETRTVVEFDFTGPNGRRVWLLLERHDVSVCV
                     TPPGFDADLIVRADLALFYRVWLGHVEYNAALRCGSVVVEGLPALAKQLPRWFMWSPM
                     ARFVREREHASSHTSAQLSANRPAV"
     gene            1809..2723
                     /locus_tag="AC16CY_06865"
     CDS             1809..2723
                     /locus_tag="AC16CY_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3353540.1"
                     /translation="MPGIGPHITTFSPLGDYAYVSGMRNGDLAILRADDRQVVRTLHL
                     GPTLTHQAKPSPDGSVLLVAQLASMSLIKVAADEAAETWDVVGSLSFAALGRAPICSV
                     FRDDGDRAYVSLRPSGIAIVDVPTMTHIATLTTDGFVACGMIKSKRGRIVTVASSGGG
                     GHIYRLDTVTDTLVDAGTLGAADWHSFNMSPNEKTGFGTSPDSDEIILADLTGPVASQ
                     IAALQIDPTAGSGNDEPDALSVRGNTLFVSLRASGQLAIVKVNQQAVTFLRLSPPAAF
                     NPLTCGPPLPQPPNGQGGCAIHGVTVRP"
     gene            2832..>3418
                     /locus_tag="AC16CY_06870"
     CDS             2832..>3418
                     /locus_tag="AC16CY_06870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3353541.1"
                     /translation="MFSAADAYERFMGRWSSELAPLLVTFADIHDGDAVLDVGSGTGA
                     LTAAIAAAAPSSRVVGIDPAASYVVFAQGRHPGDSIRFEVGDAQALRFLDGSFDRTLS
                     LLVLNFIPDPAKALREMIRVTRSGGVVAAAVWDYGEEMEMLRVFWDEAVALDPAADAR
                     DERHMPLCRKGELAAFWREHGLRDVSEEALTIQTR"
BASE COUNT          598 a         1096 c         1087 g          637 t
ORIGIN      
        1 tgtttcatgt tccgcgcgcc gatgcgcatg tcggcccttg ccttcggcac gacgatcgtg
       61 ctgtcgacct tcgcgctggg tgttcactgg attccggaca tgattgccgg cttcgcgctc
      121 gggatcgcga gtttgctgct ggcctggcgg atcgtgcgac agggccgcgc tccgttcctc
      181 gccggcgagc gcctggccgt tgacgctctc taggcgaggc tatacgtgag gacttgaaac
      241 cactgcgcta tcgatgacgc gagtgcgcca actggagggt tgggttggcc ccgaccgaat
      301 gcagggccaa ccacgagagt ccgctctcct acccgacgat cgtcagttat tccccttgca
      361 gagtccgggg actccgaact cagggatgcc tcggccctgg actccgggct cgagcccgta
      421 atacttgaac gcgtccgccg cggtttgaac ctgcgacacg ttctgtccct cgtgctggtc
      481 gaagtcattg agtccgctgg cggcgcacgc cgattgtgcg tgatccagcg cgccagtctc
      541 gttcgtgtta ttgatggtgc ccggcgggcc tttcacctcg ccagcgaacg ctccgcctgc
      601 cgcgagcacg gcaagagaag tcgcaacaac aagtagtcgt ctcatggttt cagcctcgtt
      661 tctttccttc tttgatcagc tcaagacgat accccgtaga gttggcgcgg gcaacctgca
      721 tccgaggact tggaaccgca gctcgttacg aaaacgtaat ggcgcgcgaa gagctcagac
      781 tgcagggcgg ttggcactca actgagcgga cgtgtgcgac gacgcatgct cgcgttcgcg
      841 aacgaagcgc gccatcggac tccacatgaa ccaccgcgga agctgcttcg caagagcggg
      901 cagcccttcg acgaccaccg atccacagcg aagagcggcg ttgtactcga catgtcccaa
      961 ccagacgcga tagaagagcg cgagatcggc gcggacgatc aggtcggcgt cgaatcctgg
     1021 aggcgtcacg cacaccgaca cgtcgtggcg ctcgagcagc agccacacgc gccgtccgtt
     1081 gggaccggtg aagtcgaact ccacaacggt acgcgtctcg ggcaacagct cacgattgat
     1141 ccgctggtgt atcttccaca ccagcaaccc ggcgtccaac tcctccggcc tcggatcgcc
     1201 gaacgcccag cgcacgcccc acgcgccaag ggcctcgatg accgccttca gctcccgtcc
     1261 gccttcggac aagtgatact ccgtcatcgt cgattgcact ctggggaggc gctcgacgac
     1321 gcccgagtct tcgaggtccc ggagacgtga cgagagcagc gagcgcgaga tacccgggag
     1381 cccgcgctcg atctcgttga accgatggct cccgagcacg agctcgcgca cgatgagtgg
     1441 cgtccatcga tctgcgagaa tctccgacgc ccgtgcgacg ggacagtact gccggtattt
     1501 ctgcataggc catcctggca cggccgccct gtccctgcca gtccgaaaat tggaccgaat
     1561 cggtccgatc gttacacttc cgcccgactc cagccgcgca taccgtgacc ccctgaggca
     1621 attggcagga ggtcaccatg gctcgtgtaa tgggaaggct tgcgatcgtg ttgtcggccg
     1681 tgttcagtgc gtctgttgtc gcagggacgg cccaggaatc cgattccggg gaactctggg
     1741 tcacaagtca aggcacggac cgtctgttcg tcgtccatgg agaagggtca gcaatcgaaa
     1801 cggttcatgt gcctggcatc ggcccgcaca tcacgacgtt ctcgccgcta ggcgattacg
     1861 cctacgtgtc gggaatgaga aacggcgacc tggccatcct gcgcgctgac gaccggcagg
     1921 tcgtgcggac gctgcacctg ggtccgacgc tgactcacca ggccaagcca tcgccggatg
     1981 gatcagtgct cctcgtcgcc cagctggcat cgatgagtct catcaaggtg gccgcagatg
     2041 aggcggctga aacctgggac gtggtcggga gcctctcttt cgccgcattg gggagagcgc
     2101 cgatctgttc cgtcttccgc gacgacggcg accgggcgta tgtctcgttg cggccgagcg
     2161 ggatcgccat tgtcgatgtg cccacgatga cccatatcgc cacgttgacc acggacggtt
     2221 ttgtggcctg cggcatgatc aaatccaagc gaggccggat cgtcaccgtc gcctccagcg
     2281 gtggaggagg tcacatctat cggctggaca ccgttaccga tacgcttgtc gatgccggta
     2341 cgctcggcgc ggccgattgg cacagcttca acatgagccc gaacgagaag acggggttcg
     2401 gaacgtcacc agatagcgac gagatcatcc tggcggatct cacagggccc gtggccagcc
     2461 agatcgctgc gttgcagatc gatccgacgg cgggcagcgg aaacgatgag ccggacgccc
     2521 tcagcgtgcg cggaaacacc ctcttcgtat cgttacgcgc atcaggtcag cttgcgatcg
     2581 tgaaggtcaa ccagcaagcc gtcacgttcc ttcgactttc tccgcctgcg gcgttcaacc
     2641 cgttgacgtg cggcccgccc cttccgcagc ccccgaacgg gcaaggggga tgcgcgatcc
     2701 acggcgtcac ggtgcggccg tagtgggggc atgtgggcac gcgttgtcag cagcctccat
     2761 tgctcgctct cgtcacgctc atggcggtga gtctctcagc cgggactgac ggagccggca
     2821 ggcagggtgg gatgttctca gcggcagacg cgtacgagcg cttcatgggg agatggagta
     2881 gcgagctcgc cccgctgctc gtcacattcg ccgacattca cgacggtgac gccgtcctgg
     2941 acgttggatc gggaaccggt gctctgacgg cggcgatcgc cgcggccgcc ccgtccagtc
     3001 gcgtcgtcgg cattgatccc gccgcgtcgt atgtcgtttt cgcccagggg cgtcatccag
     3061 gggattcaat ccggttcgag gtcggcgatg cccaggcgct gcggttcctc gacgggagct
     3121 tcgaccggac gctctccctt ctcgtgctca atttcatccc cgatcccgcg aaagcgctgc
     3181 gcgaaatgat tcgagtcacg aggtccggcg gcgtcgtcgc ggcagccgtc tgggactacg
     3241 gggaggagat ggagatgctg cgcgtgttct gggacgaggc ggttgcgctc gatcccgccg
     3301 cggacgcgcg cgacgaacgg catatgccgc tctgccgcaa gggagagctt gccgcattct
     3361 ggcgcgaaca cgggcttcgg gatgtgtccg aagaggcgct cacgattcag acgcggtt
//
LOCUS       DBNGSH010000293         2398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3419037, whole genome shotgun sequence.
ACCESSION   DBNGSH010000293 DBNGSH010000000
VERSION     DBNGSH010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3419037"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..1270)
                     /locus_tag="AC16CY_06875"
     CDS             complement(293..1270)
                     /locus_tag="AC16CY_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3353542.1"
                     /translation="MDTSDQRADRQRGSMTLAVIVCALAGGLTVGAALVSSRADDGGS
                     RGAVASIATEEYGRRLIAQTSELIGPDHPDPAMRYSGIRMACGSCHLGVGTEPGTLSL
                     LQAAANYPRFSGRDGGVRDLVDRVNGCMTRSMNGRELPRDSPEMIAITAYITSLGDLY
                     AAMGEGQRTASEQLPFATPNRAADLKAGRQVFEERCATCHRPDGAGLQATPERMDGYV
                     FPPLWGPDSFNNGAGMHRVLTAARFIKAKMPLGQADLTNDQAFDVAAYINSQPRPQMA
                     GLERDYPDRSTKPIDGAYGPFADDFPLEQHRFGPFQPIERHYKSPKKTN"
     gene            complement(1351..1518)
                     /locus_tag="AC16CY_06880"
     CDS             complement(1351..1518)
                     /locus_tag="AC16CY_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353543.1"
                     /translation="MIASIIVFGSIVFAAGFCLAWLARPEVRAWLETPKHRFQASVRQ
                     YDDTAVRSVRL"
     gene            complement(1515..>2398)
                     /locus_tag="AC16CY_06885"
     CDS             complement(1515..>2398)
                     /locus_tag="AC16CY_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3353544.1"
                     /translation="TDDWHFAHLYAPRSVVSASVMPSYAWLFDGAPDRPRQSARDLVD
                     YLQSLGRAREVAGPEGEARAREGCNCPDDAMAQMAFNAALNASAARPRRGADAPALAR
                     SGDQARGQILYAANCASCHGARGHGDGPGASSLRPRPTDLSAHEYTLDRLSDVLWNGV
                     AGTAMQAWRDHPLEDLSAMARAVKNFQVPQEAIVPETFVALGQRVYAANCVQCHGVNG
                     RGDGSAARELSTAPTDFTRQRPTLGYSIAVMRNGVEGTRMASWTGRISEAELVAVANY
                     VRTLFEPDEAPAGGRTQ"
BASE COUNT          373 a          801 c          824 g          400 t
ORIGIN      
        1 acgctcgctg ggaccgcgac gatcgacgct gcgatcccgg cggcgaacag cacggcgccg
       61 accatcgtcg cgatgggttg ccacaggcgt cgaaggaggt gtcgtcgcgg gtgatcgagg
      121 acgaccctgg ctggcatcct cgctgaattc tagatggccg cgtgactatt ccggagttca
      181 cttgtgttga catcgtaggg gccggtcttt agaccggccc aaccgggtct gccttcgact
      241 tcgctcaggc cagggaagac ccgcctctac atcaacacaa gtgaactttg gtttagttgg
      301 ttttcttggg cgacttgtaa tgccgttcga tcggctggaa ggggccgaag cggtgctgct
      361 cgagtggaaa gtcgtcggcg aacggaccgt acgcgccgtc gatcggcttg gtcgaacggt
      421 cggggtagtc gcgctcgaga ccggccatct gcggccgggg ttgtgagttg atgtacgccg
      481 caacgtcgaa cgcctgatcg ttggtgagat cggcctgccc gagaggcatc ttggccttga
      541 tgaagcgcgc cgccgtcagc acgcgatgca tgccggcacc gttgttgaag ctgtcggggc
      601 cccagagcgg cggaaagacg tatccgtcca tccgctccgg cgtggcctgc aggccggcgc
      661 cgtccggccg gtgacaggtg gcgcaccgtt cctcgaacac ctgtctgccc gccttcagat
      721 cggccgcgcg attcggcgtg gcgaacggca gctgttcgct ggccgtccgc tgaccttcgc
      781 ccatcgcggc gtacagatcg cccagggacg tgatgtaggc cgtgatggcg atcatctccg
      841 ggctgtcccg cgggagctcg cgcccgttca tgctgcgcgt catgcagccg ttgacgcgat
      901 cgacgagatc gcggacgccg ccgtcccgtc ccgaaaatcg cgggtagttg gccgccgcct
      961 gcagcagcga caaggtcccg ggctcggtgc cgacgccgag atggcacgac ccgcacgcca
     1021 tccggatccc ggagtagcgc atcgcggggt cgggatggtc gggtccgatg agctcggacg
     1081 tctgcgcgat cagccggcgg ccgtactctt cggtggcaat cgaggccacc gcgccgcgcg
     1141 agccgccgtc atctgccctg gagctcacaa gcgcagcgcc gacggtgagc ccgccggcaa
     1201 gggcgcacac gatgacggcc agggtcatgc tcccgcgttg gcgatccgcg cgttgatcag
     1261 atgtgtccat cgcgtcacgg tccggctgcc gccttcgcgc ttcgcgcttc ggcgcccaag
     1321 aaagccggac actacgtctg tcaggtctgg ctaaagccgg acactacgta cggcagtgtc
     1381 atcatactgc cgcacgctcg cctggaagcg gtgcttcggc gtttccagcc acgcccggac
     1441 ctccggcctc gcgagccacg caagacagaa cccggcggcg aagacgatcg acccgaacac
     1501 gatgatgctg gcgatcactg tgtgcgccct ccggcaggtg cctcgtccgg ctcgaagagc
     1561 gtgcgcacgt aattggccac ggcgacaagc tcagcctcgc tgatgcgtcc cgtccacgac
     1621 gccatgcgcg tcccttcaac gccgttgcgc atgacggcga tgctgtaacc gagcgttggg
     1681 cgttgacggg tgaagtcggt cggcgccgtc gagagctcgc gggccgccga gccgtctcct
     1741 cgtccgttga cgccatgaca ctgcacgcag ttggcggcgt agacgcgttg cccgagcgcc
     1801 acgaaagtct cgggaacgat cgcctcctga ggcacctgga agttcttcac cgcgcgcgcc
     1861 atggccgaga ggtcctcgag cgggtgatcc cgccaggcct gcatcgccgt gccggccact
     1921 ccgttccaca gcacatcgct caaacgatcg agcgtgtact cgtgcgcgga gaggtcggtg
     1981 gggcgagggc gcagcgacga ggcgcccggg ccatccccgt gtccccgcgc tccgtgacag
     2041 cttgcgcagt tggccgcgta gaggatctgt ccgcgagcct gatcgccgct acgggcgagc
     2101 gccggcgcgt ccgcgccacg acgcggccgc gcagcactcg cgttcagcgc tgcgttgaac
     2161 gccatctggg ccatcgcgtc gtcggggcag ttgcagccct cgcgggcccg ggcttcgcct
     2221 tcggggccgg cgacctcgcg cgcgcgcccg aggctctgga ggtagtccac gaggtcgcgc
     2281 gccgactgtc gcggacggtc cggcgcaccg tcgaagagcc aggcatatga aggcatgacg
     2341 gaggccgaga cgacagatcg gggcgcgtag agatgcgcga aatgccagtc gtcggtcc
//
LOCUS       DBNGSH010000294         1820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2569993, whole genome shotgun sequence.
ACCESSION   DBNGSH010000294 DBNGSH010000000
VERSION     DBNGSH010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2569993"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="AC16CY_06890"
     CDS             <1..816
                     /locus_tag="AC16CY_06890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353545.1"
                     /translation="MKAWSLSWNTADVPLAGTTTQVVQVRRTGKGCLSYVAGSGDEAV
                     VIDPSLANEVYLDIARRHGWRITHVLDTHVHADHLSRARSLSAAAGAILRLPAQRRVH
                     FPFTAVADGDRITFGQAVLFARRTPGHTDESTSFLLNGEAVFTGDTLFTRAVGRPDLK
                     ADLEGARLRARALFASLTWLRSLGPDVLVLPGHAGELVAFGPEVEAARLREVDGWLSE
                     WLVSERVFVDRLVSRLPPTPPNYTRIVELNEQGELPAGDPTDLEAGANRCAVA"
     gene            830..1486
                     /locus_tag="AC16CY_06895"
     CDS             830..1486
                     /locus_tag="AC16CY_06895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3353546.1"
                     /translation="MSSCCSAYGGVADLQFDAARVALELRQYRKKGAGPTTRLLRDGL
                     VAAGVVNGSLLDIGAGIGALTFDLLDRGCASAVVVDASSAYLVAAAEEATRRDRVDRI
                     RFVKGDFVDLAVKLDHADVVTLDRVVCCYPFVERLLVPAVGHARRAIAISYPRDVWYV
                     RAVLGFDNLRRRLTGNPFQTFVHPPALMERLILGAGFRLSSRRTTWTWASDVFVRTPA
                     "
     gene            1595..>1820
                     /locus_tag="AC16CY_06900"
     CDS             1595..>1820
                     /locus_tag="AC16CY_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3353547.1"
                     /translation="MAPAKAPPPLAIRWAVLFFLSVAMFGNYYVYDSIAPLADILQTQ
                     LGFSSTQVGALNGIYHFPNIAMVLIGGILVD"
BASE COUNT          254 a          604 c          623 g          339 t
ORIGIN      
        1 atgaaggcgt ggagcctgtc gtggaacacg gcggacgtcc cgcttgccgg cacgacgacg
       61 caagtcgtgc aggtgcgccg aaccggcaag ggctgcctct cgtacgttgc gggctccggc
      121 gacgaagccg tcgtgatcga cccttcgctg gcgaacgagg tctatctcga catcgcccgc
      181 cgtcatggct ggcgaatcac gcacgttctc gacacgcacg tccacgcgga tcatctgtcg
      241 cgcgcgcgat cgctgtccgc cgcggccggc gccattctgc ggctgcccgc ccagcggcgc
      301 gtgcattttc cgttcacggc ggtggccgac ggcgatcgca tcaccttcgg ccaggccgtg
      361 ctcttcgcgc gccgcacgcc cggacacacc gacgagagca cgtcgttcct gttgaacggc
      421 gaggcagtct tcacgggaga cacgcttttc acgcgggcgg ttggaagacc tgatctgaaa
      481 gctgacctcg agggcgcacg ccttcgggca agggcgctgt tcgcgtcgct gacgtggctg
      541 agatcgctgg ggcctgacgt gctggtcctc ccggggcacg ccggcgagct ggtcgcgttc
      601 ggcccagagg tcgaagcagc gcgcctgcgt gaggtcgatg gctggctctc ggagtggctg
      661 gtctcggagc gtgtgttcgt ggaccgtctc gtgtcgcgtc tgccgcccac tccaccgaac
      721 tacacgcgca tcgtggagct caacgagcag ggcgagctgc ccgcggggga tcccactgat
      781 ctggaggctg gcgccaaccg ctgcgcggtg gcgtgaggat cgagcgagcg tgagcagttg
      841 ctgttctgcg tacggcggcg tcgccgatct gcagttcgat gcggcaaggg tggcgctgga
      901 gctgaggcaa taccggaaga agggcgccgg accgaccact cgtttgctgc gtgacggact
      961 cgtcgcggcg ggcgttgtca acgggtcgct gctcgacatc ggcgcgggaa tcggcgcgct
     1021 gacgttcgac ctgctcgatc ggggctgtgc gtcggccgtg gttgtcgatg cctcctccgc
     1081 ctacctggtg gccgccgccg aggaggccac gcgccgcgac cgggtcgacc ggatccggtt
     1141 cgtcaagggc gacttcgtgg acctcgcggt gaagctggat cacgccgacg tggtgacgct
     1201 cgatcgcgtg gtctgctgct atcccttcgt cgagcggctg ctggttcccg cggtcgggca
     1261 cgcccgccgt gcgattgcga tctcgtatcc ccgtgatgtc tggtacgtcc gcgcggtcct
     1321 cggattcgac aacctgcggc ggcggttgac gggcaacccg ttccagacat tcgtgcaccc
     1381 gccggcgctg atggaacggc tcattctcgg agcggggttc cgcctgtcga gtcgccggac
     1441 gacgtggacg tgggcgtcgg acgtgttcgt tcggacaccg gcttgaggca tacttcatcg
     1501 gtcctgtcga gcctggccct gtcacggtcc ggctgaagcc ggacactacg tctgccatga
     1561 tgccggacac gacctctcgc cgatgagcgc tggaatggct cccgcaaagg cccctccgcc
     1621 cctcgcgatc cgctgggccg tgctcttctt cctcagcgtc gcgatgttcg gcaactacta
     1681 cgtgtacgac tcgatcgcgc cgctggccga cattctccag acgcagctcg ggttcagctc
     1741 gacgcaggtc ggagcgctca acggcatcta ccacttcccc aatatcgcga tggtcctcat
     1801 cgggggcatc ctcgtcgatc
//
LOCUS       DBNGSH010000295         2614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_872246, whole genome shotgun sequence.
ACCESSION   DBNGSH010000295 DBNGSH010000000
VERSION     DBNGSH010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_872246"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="AC16CY_06905"
     CDS             <1..280
                     /locus_tag="AC16CY_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353548.1"
                     /translation="GIRHILHDYATFNWIMTGTDVFAVEGTSAGEYKDGPWRAGVTHA
                     GRWCDVFEVRDFLIQRCFIYLDPDYAGLDTARYPWLSAGGRDPSKPMG"
     gene            complement(366..1445)
                     /locus_tag="AC16CY_06910"
     CDS             complement(366..1445)
                     /locus_tag="AC16CY_06910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3353549.1"
                     /translation="MIRCDGLVVGGGPAGSTCARLLRRAGWDVVVADRARFPRDKVCA
                     GWLTPDVFPLLDLTPAEYRATGLTLQEVTGFRTGVIGGRLVETRYPSVVSYAIRRCEF
                     DDFLLRRAGVRVLDNTPVTSFRREDSRWIVNDAIDTAMVIGAGGHFCPVARHLRGGAD
                     EEAPVVAKEAEFLLDGHETEVSQSTPELFFCRDLQGYGWCVRKGNYVNVGIGRRDSSG
                     FGTHVKDFAAFLEKNGILIGASEPRWRGHAYLASGTGARPLVGPGMLLVGDAAGLAYP
                     ESGEGIKPAIASGRLAAETLVAAGRHATIGDLQPYVTSLRRLYPAAPQKPAPVRAAVA
                     AIGRVLLGSHAFTRHVVLDRWFLRV"
     gene            complement(1442..>2614)
                     /locus_tag="AC16CY_06915"
     CDS             complement(1442..>2614)
                     /locus_tag="AC16CY_06915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3353550.1"
                     /translation="AAQVQQTIDPAAVRLELWDGSSTYAGSRAPIGDLVVRDRRTLAG
                     LAVNPDLWFGEAYMAGRLEVRGPLEPVVEALSGLTSGGPSWRDRVRLALAGANSLRDS
                     RKNVHHHYDLGNAFYQLWLDRELVYTCAYFAEPQMSLDDAQIAKLDLVCRKLRLRPGD
                     TVVEAGCGWGALALHMARRYGARVKAFNVSREQLEYARSRASREGLAGQVEFIDDDYR
                     NVSGQFDAFVSVGMLEHVGLKHFHSVADVLRRTIRPDGGRGFLHFIGRDIARPLNAWI
                     ARRIFPGAYTPTLAEVTTGILAPAGMSTIDVENLRLHYARTLACWSERFAAAREQVRA
                     TYGATFERAWELYLAGSQAAFATSSMQLFQVLFVPSRSAPPALTRADIYEAGGTAT"
BASE COUNT          420 a          923 c          884 g          387 t
ORIGIN      
        1 cggcatcagg cacatcctcc acgactacgc caccttcaac tggatcatga ccgggaccga
       61 cgtcttcgcc gtggaaggca cgagcgcggg cgagtacaag gacgggccct ggcgcgccgg
      121 ggtcacgcac gccgggcgct ggtgcgatgt gttcgaggtc cgcgacttcc tgattcagcg
      181 ctgcttcatc tacctcgacc ccgactacgc ggggctcgac acggcgcggt acccgtggct
      241 gtccgcgggt ggccgcgatc cgtcgaagcc gatggggtga gcctgggccg cgtcggctga
      301 agccgacgct ctacggcttg gggtgtgttc cgtagagcgc acgcttcagc gtgcgcgttc
      361 gggcgctaga cccgaaggaa ccagcgatcg agcacgacgt gcctcgtgaa cgcatgagag
      421 ccgagcagca cgcggccgat ggcggccacg gccgctcgca cgggagcagg tttctgcggg
      481 gccgcgggat agagccggcg aagcgatgtg acatacggct gaaggtcccc gatcgtcgcg
      541 tgccttcccg cagcgaccag ggtttccgcc gcgagccgtc cggaagcgat cgccggcttg
      601 atgccctcgc cgctctccgg gtacgcgagg cccgcggcgt cgccaaccag cagcatgccg
      661 gggccgacga gcgggcgcgc gccggtgcca gacgcgagat acgcgtgccc gcgccatcgc
      721 ggttcggagg cgccgatgag gatcccgttc ttctccagga acgcggcgaa gtccttcacg
      781 tgcgtgccga atccgctgct gtcgcggcgg ccgatgccga cgttgacgta gttccctttc
      841 cgcacgcacc acccgtagcc ctgcaggtca cggcagaaga acagctccgg cgtgctctgg
      901 ctcacttcgg tctcgtggcc gtcgagcagg aactccgcct ccttcgccac gacaggggcc
      961 tcctcgtcgg cgcccccgcg caggtggcgc gccaccggac agaagtgtcc gcccgctcca
     1021 atcaccatcg ctgtgtcgat cgcgtcgttg acgatccagc ggctgtcttc gcggcgaaac
     1081 gacgtcaccg gcgtgttgtc gagaactctg actccggccc ggcgcagcag gaaatcgtcg
     1141 aactcgcatc gccggatcgc gtagctgacg acactggggt agcgcgtctc gacgaggcgc
     1201 ccgccgatga cgcccgtgcg gaaaccggtg acctcctgaa gcgtgagccc ggtcgcgcgg
     1261 tactccgccg gcgtcagatc gagcagcggg aagacgtccg gcgtcagcca gcccgcgcac
     1321 accttgtcgc gcgggaaccg cgcgcgatcg gcgacgacga cgtcccagcc ggctcgacgg
     1381 agcagccggg cacacgtcga tccggccggg ccaccgccga cgacgaggcc gtcgcagcgg
     1441 atcatgtggc cgtcccgccg gcctcgtaaa tatccgcgcg cgtcaacgcg gggggagcgg
     1501 accggctcgg cacgaacagc acctggaaga gctgcatcga gctggtcgcg aacgccgcct
     1561 gcgagccggc caggtacagc tcccacgcgc gctcgaaggt ggcaccgtac gtcgcgcgca
     1621 cctgttcgcg agcggcggcg aaccgctcgc tccagcaggc aagcgtgcgc gcgtagtgaa
     1681 gccgcaggtt ctccacgtcg atcgtggaca tgccggccgg cgccaggatg ccggtcgtca
     1741 cttccgccag cgtaggcgta tacgcaccgg gaaagatgcg gcgcgcgatc cacgcgttga
     1801 gtgggcgcgc gatgtcgcgg ccgatgaaat gcaggaagcc gcgtccgccg tccggtctga
     1861 tggtgcggcg cagcacgtcg gccacgctgt ggaagtgttt cagtccgacg tgctcgagca
     1921 tgcccaccga aacgaacgcg tcgaattgcc ccgagacgtt ccggtagtcg tcgtcgatga
     1981 actcgacctg tcccgcaagc ccctcgcggg acgcgcggct gcgcgcgtac tcgagctgct
     2041 cgcgcgacac gttgaacgcc ttgacgcgcg cgccgtaccg ccgcgccatg tgcagggcca
     2101 gggcccccca gccgcacccc gcctcgacca cggtgtcgcc cgggcggagc cgcagctttc
     2161 ggcagacgag atcgagcttc gcgatttgcg cgtcatcgag cgacatctgc ggctcggcga
     2221 agtaggcgca ggtgtacacg agctcgcgat ccagccagag ctgatagaac gcgttgccca
     2281 gatcgtagtg atggtggacg ttcttcctcg aatcacgcag cgagttggcg ccggcaaggg
     2341 ccagcctcac ccgatcgcgc cacgacgggc ctcccgacgt caggcccgac agcgcctcga
     2401 cgaccggttc cagaggtcct cgtacctcga gccggccggc catatatgcc tccccgaacc
     2461 agaggtcggg gttgacggcc agtcccgcca gcgtgcgcct gtcgcgcacc acgagatccc
     2521 cgatcggggc acgcgatccc gcataggtcg agcttccgtc ccacagctcc agcctcacag
     2581 ctgccggatc gatcgtctgt tgtacctgcg cggc
//
LOCUS       DBNGSH010000296         5260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1551764, whole genome shotgun sequence.
ACCESSION   DBNGSH010000296 DBNGSH010000000
VERSION     DBNGSH010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1551764"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1724
                     /locus_tag="AC16CY_06920"
     CDS             <1..1724
                     /locus_tag="AC16CY_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HIR3353551.1"
                     /translation="KKGAGGRGQGARGKGAGDKGAGGRGQGAGGKGEGGKGAGGKGAG
                     GSHYVNVHRAVTAGSNVSFAGTDITAGETVLRRGQVLTSRDTGVLAAIGVAKVNVWRR
                     PIVAILSTGDEIIAPGEPMQPAHIYDSNAQVLADAVRELGGEPRRLGITPDDEPALRV
                     KVREALEIADIVLLSGGTSKGAGDVSYRVVAELTDPGIVAHGVALKPGKPICLASTGG
                     RPVVVLPGFPTSAIFTFHEFVAPVLTSMAGRSTSARATVQAKLAVKVNSEIGRTEYLL
                     VGLVETPDAAGLAEAGHDEKRNGVGSHFSSLAAYPMGQGSGSVTTFSRADGFTTIDRH
                     QEIVPAGTLLTVQLLGNELQLADLVVIGSHCIGLDYLLGELDKTGVRPKFLAVGSLAG
                     LEAARRGECDIAGVHLLDPQSGEYNLPFLTPALELIRGYGRLQGIAFRKGDARFEGRT
                     AAEAIASATSDASCVMVNRNQGSGTRALVDRLLDGAKPAGYAVQPRNHNAVAAAVVQG
                     RADWGVTLDTIARSAGLGFIRVQPEQYDFVVPKVRAGRAGVAAFRSLLTRADTRDALV
                     RLGMTPR"
     gene            1721..2317
                     /gene="mobA"
                     /locus_tag="AC16CY_06925"
     CDS             1721..2317
                     /gene="mobA"
                     /locus_tag="AC16CY_06925"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR3353552.1"
                     /translation="MTPGGIVLCGGKSTRMGTSKALLPFGPETMLQRVVRILGEIVTP
                     IVVVAAGDQELPELPAGVIVTRDEREGRGPLEGLRAGLKALPPDVDAAYVTSCDVPLL
                     VPGFVRQMLDLARGYDVAVMEIDGFTHPLSAVYRRSTLACVEELLAKNRLRPAFLFEG
                     VNTRRIGPREMTADADLRTLRNLNTREDYERALVEAGL"
     gene            complement(2331..4202)
                     /locus_tag="AC16CY_06930"
     CDS             complement(2331..4202)
                     /locus_tag="AC16CY_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HIR3353553.1"
                     /translation="MLAVMPLTKVHSASFLHLNPGGVSDLPETIPVNIVFVGYDAPLI
                     DQASFLSALPQTYRPIVRSRYWYGIVEELGLQYGFNYNVTFTDAAWEDSLFAALSALA
                     TPAPRTLFQDQYNAQIHNVLDVGTNHFIDAPSVEQWLIDHAPAGVDTQRNTIFFINWW
                     GRPDFKFHVYTKFGEADPDTGYDFGVNRQSRKIIAWGGTTPDDEESGLGARGERRVWF
                     HDLSAGPESWTDNWNVDDADVDGDGVADYRLPPTWEYLTAGGYRAAAALSPDLARVAR
                     FVAINLLFASSPLYPPYLSPLRLPGSLNLDLNTYEGWNGVDASQAYQTPSLLVDEVSE
                     LHRLPYTLDQQDLAFKQGARNCHQLWLNDNACYPNRPYPAFANLFLYSAFTLGQTRDG
                     GGDYEGMLFNYVTGDNRSSGLLGFADDNWVDGTQSGVFSFLSPVITSLGYGLTTTEIH
                     EFGHHIGMSHPHDGWDSELETDYGPEGDFYFAWSGDQSNSVMSYVDLNWDFSQFDRDN
                     FNRSHAAGYIVNANAIAEAVLASPNAGLGLAELDAADTEVGLAKGRLAVHDYAATFDH
                     ARLAYLHALRAATLASVNVPASDNGWVVAPALRGRSPVGRTYSHVDRYGPGTKRSDQ"
     gene            4414..4797
                     /locus_tag="AC16CY_06935"
     CDS             4414..4797
                     /locus_tag="AC16CY_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353554.1"
                     /translation="MTRRSFFLSLLAVCSPLVLVVGCGGSSEEEANAPFTVTYSSTYL
                     TIRNQLGSAITEGQIELVPSGVLAPFRTNLPRIEPGSSRDILFTQFGGTGGARFMRGS
                     TRIKFARVTATDSVGTQHKREIPFE"
     gene            complement(4962..>5260)
                     /locus_tag="AC16CY_06940"
     CDS             complement(4962..>5260)
                     /locus_tag="AC16CY_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3353555.1"
                     /translation="TADPKHYSVSFENDVARFLRVKYGPGEKSAMHRHLPGCVIFLTD
                     QTFNFTIPDGTTEPAMVPAGALGCSDGNVHAAENIGDEAEFIMVEFKDRSAFRK"
BASE COUNT          898 a         1663 c         1811 g          888 t
ORIGIN      
        1 tgaagaaggg ggcagggggc agggggcagg gggcaagggg caagggggca ggggacaagg
       61 gggcaggggg caggggccag ggggcagggg gcaaggggga agggggcaag ggggcagggg
      121 gcaagggggc agggggcagc cactacgtca acgtccaccg tgccgtgacg gcgggcagca
      181 acgtctcttt cgcgggcact gacatcactg ccggtgagac ggtgcttcgc cgcggacagg
      241 tgctgaccag ccgcgacact ggcgtcctcg cggcgatcgg cgtcgcgaag gtaaacgtct
      301 ggcgcaggcc gatcgtcgcc atcctgtcca caggcgacga gatcatcgcg cccggcgagc
      361 cgatgcagcc ggcccacatc tacgactcga acgcacaggt gcttgccgac gccgttcgag
      421 agctgggcgg cgagccccgc cgcctcggca tcacacccga cgacgagcct gcgctgcggg
      481 tgaaggtgcg cgaggcgctg gagatcgccg acatcgtgtt gttgtcgggg ggcacgagca
      541 agggggccgg cgacgtgtcc taccgcgtcg tcgccgagct gaccgatccc ggcatcgtcg
      601 cgcacggcgt ggcgctcaag ccgggcaagc cgatctgtct ggcctcgacc ggcggacgac
      661 ccgtcgtcgt gctgcccggc ttcccgacgt cggccatctt cacgtttcac gagttcgtcg
      721 ccccggtgct cacctcgatg gccggccgca gcacgagcgc tcgcgcgacg gtgcaagcaa
      781 agcttgcggt caaggtgaac agcgagatcg gccggacgga gtatctgctg gtaggactgg
      841 ttgagacgcc agacgctgcc ggtctggctg aagccggaca cgacgaaaaa cgtaatgggg
      901 tcgggagtca tttttcgtcg ctcgcggcgt accccatggg acaagggtct ggcagcgtca
      961 cgacgttcag ccgcgccgac ggattcacca cgatcgatcg ccaccaggag atcgttcccg
     1021 ccgggacgct cctcacggtg cagttgctcg ggaacgagct tcagctggcg gatctcgtag
     1081 tgatcggcag ccactgcatc ggcctcgact acctgctcgg cgagttagac aagacaggcg
     1141 tgcgcccgaa gttcctggcc gtgggcagcc tcgccggcct ggaggccgcc aggcgcggag
     1201 agtgcgacat cgcgggcgtt cacctgctgg acccgcagag cggcgagtac aacctcccct
     1261 tcctgacgcc cgctctcgag ctgatacgag gctacggacg acttcagggg attgcctttc
     1321 gcaagggcga cgcgcgcttc gaagggcgga cggcggccga ggcgatcgcc tcagcgacct
     1381 cggatgcgtc gtgcgtgatg gtgaatcgca accagggaag cggcacccgc gcgctcgtcg
     1441 atcggctcct cgacggcgcg aagcctgcgg gctacgccgt gcagccgcgc aatcacaacg
     1501 ccgtcgccgc cgccgtcgtg caggggcgcg ccgactgggg cgtgacactc gacacgatcg
     1561 cgcgaagcgc cgggctcggc tttatccgcg tgcagccaga gcagtacgat ttcgtcgtgc
     1621 ccaaggtccg cgccgggcgc gccggtgtcg ccgccttccg atcgctgctg accagggctg
     1681 atacccgtga cgcgctcgtc cggctgggaa tgacaccccg gtgactccag gcggcatcgt
     1741 gctgtgcggc ggaaagtcca cgcggatggg cacttcgaaa gcgctcctcc ccttcggccc
     1801 cgagacgatg ctgcagcgcg tcgtccggat cctcggcgag atcgtcacgc cgattgtcgt
     1861 ggtggcggca ggtgatcagg agttgccgga attgccggct ggcgtcatcg tcacgcgcga
     1921 cgagcgcgag gggcgcggac cgctggaagg gctgcgcgcg ggattgaagg cgctgccgcc
     1981 cgacgtcgat gctgcgtacg tgacgagctg tgatgtaccg ctgctcgtgc ccggctttgt
     2041 caggcagatg ctcgatctcg cccgtggcta cgacgtggcg gtgatggaga tcgacggttt
     2101 cactcacccg ctctcggccg tctaccgccg ttcgactctg gcctgtgtcg aggagctgtt
     2161 ggcaaagaac cgcctacgcc cggcatttct cttcgaggga gtgaacacgc gacggatcgg
     2221 tcctcgcgaa atgaccgccg atgcagacct gcgcacgctg cggaatctca acacgcgcga
     2281 ggactacgag cgcgcgctgg tggaggcggg tctttagacc cgccttaggg tcactgatcc
     2341 gatcgcttgg tgcccggtcc gtaccgatcg acgtggctgt acgtccttcc gaccggcgat
     2401 cggccgcgaa gcgccggtgc cacgacccag ccgttgtccg acgccgggac gttcacgctg
     2461 gccagggtcg ccgcacggag ggcgtgaaga tacgcgagac gggcatgatc gaacgtcgcc
     2521 gcgtagtcgt gaacagccag cctgcccttg gccaatccca cctcggtgtc ggccgcgtcg
     2581 agctccgcga gcccgaggcc cgcattcggg ctcgccagca ccgcctccgc aatcgcgttg
     2641 gcgttgacga tgtagccggc ggcgtgagac cgattgaagt tgtcccggtc gaactggctg
     2701 aagtcccagt tcagatccac gtaactcatg accgagttgc tctggtctcc ggaccaggcg
     2761 aagtagaagt cgccctcggg accgtagtcc gtctccagct cggagtccca gccatcgtgc
     2821 gggtggctca tcccgatgtg atgaccgaac tcgtgaatct cggtcgtcgt cagtccgtag
     2881 ccgagcgacg tgatgaccgg cgagaggaag ctgaagacgc cgctctgggt gccatccacc
     2941 cagttgtcgt cggcgaatcc cagaagccct gaagaccggt tgtccccggt cacgtagttg
     3001 aacagcatgc cctcgtagtc cccgcctccg tcgcgcgtct gaccgagagt gaaggcgctg
     3061 tagagaaaca ggttggcgaa cgccggatac ggccgattcg ggtagcaggc gttgtcgttg
     3121 agccagagct gatggcagtt cctcgcgccc tgcttgaacg cgaggtcctg ctggtcgagc
     3181 gtgtatggaa gccgatgcag ctcggagacc tcgtcgacga gcagcgaggg cgtctggtag
     3241 gcctgcgatg catcgacgcc attccacccc tcgtacgtat tgaggtccag gttgagcgag
     3301 cccggcagac gcaacgggga caggtacggc ggatagaggg gcgacgacgc aaagagcagg
     3361 ttgatcgcga cgaagcgcgc cacacgggcg agatccggac tgagggccgc cgcagcccgg
     3421 tagcctccgg ccgtcagata ctcccaggtg ggcggaagcc gatagtccgc aaccccgtcg
     3481 ccgtcgacat cggcatcgtc cacgttccag ttgtcggtcc acgactccgg tccggccgag
     3541 agatcgtgga accacacgcg ccgttcgccg cgggcgccaa gtccgctctc ctcgtcatcc
     3601 ggcgtggtgc caccccacgc gatgatcttg cggctctgcc ggttgacgcc gaagtcgtag
     3661 ccggtgtcgg gatcggcttc tccgaatttc gtgtagacgt ggaacttgaa gtccgggcgg
     3721 ccccaccagt tgatgaagaa gatcgtgttg cgttgcgtgt cgacgcccgc gggcgcgtgg
     3781 tcaatcaacc actgttcgac cgacggggcg tcgatgaaat gattcgtccc gacgtccaga
     3841 acgttgtgga tctgcgcgtt gtactgatcc tggaacagcg tgcggggcgc cggcgtggca
     3901 agagccgaga gcgccgcgaa aaggctgtct tcccaggccg catccgtgaa cgtcacgttg
     3961 tagttgaacc cgtactgcag accgagctct tccacgatcc cgtaccagta ccgcgatcgg
     4021 acgatcggcc ggtacgtctg cggcagcgcg gacaggaagc tcgcttgatc gatgagcggg
     4081 gcgtcatagc cgacaaacac gatgttcacc gggatggttt ccggcaggtc ggacacgccg
     4141 cccgggttga gatggagaaa cgacgcggag tgcaccttcg tcagcggcat gaccgcgagc
     4201 aagaagaggc agagagcgaa caacgatcgg ctgcgcatag tccctcccga atcccggaaa
     4261 ccacgaacgt atcgcctcgg gtggtgctcg gtcaaccccg tcatatgtaa caacttggaa
     4321 cacggggagc tgatgcataa tcacctcgcc tgtgttcgat ttggcgactt ggaattgcgc
     4381 tgcactcggg ttcgacgaca aaggaggggg cgcatgacgc gacggtcgtt tttcctgtct
     4441 ctgcttgcgg tgtgctcacc gctggtcctg gtggttggtt gcgggggctc ctccgaggag
     4501 gaagccaatg cgcccttcac cgttacgtat tcgtcgacct atctgacgat ccggaaccag
     4561 ctgggctcgg ccatcacgga agggcagatt gagctcgtgc cctcaggcgt gctggcgccg
     4621 ttccggacga atcttccccg gatagaacct ggttcgagcc gggacatcct gttcacccag
     4681 ttcggcggca ccggaggcgc caggttcatg cggggctcga cccggatcaa gttcgcgcgc
     4741 gtgacggcta ccgacagcgt cgggacgcaa cacaagcgcg aaatcccctt cgagtagaag
     4801 gcgccgctgc ggcgtgcgcg ccgtcggcga atggctcgtt cactgggccg cgcgtgcgcc
     4861 gcagcgtacg tttctcgccg agcgatccgg atcggcgacg cggtgcgcct ggtcgatgcc
     4921 gagggtgtac gccggaggtc ccggcgtcgt cgccctgggc gttacttcct gaacgccgat
     4981 cgatccttga actccaccat gatgaactcg gcctcgtcgc cgatgttctc ggccgcgtga
     5041 acgttcccgt cgctgcaccc gagcgcgccg gccggcacca tggccggctc cgtcgtcccg
     5101 tcggggatcg tgaagttgaa cgtctggtcc gtcagaaaga tgacgcagcc gggaaggtgc
     5161 cggtgcatgg ccgacttctc gcccgggccg tacttcacgc ggagaaagcg ggccacgtcg
     5221 ttctcgaagg acaccgagta gtgtttgggg tccgccgtca
//
LOCUS       DBNGSH010000297         2918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1721888, whole genome shotgun sequence.
ACCESSION   DBNGSH010000297 DBNGSH010000000
VERSION     DBNGSH010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1721888"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="AC16CY_06945"
     CDS             <1..641
                     /locus_tag="AC16CY_06945"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353556.1"
                     /translation="SEEIHALRGVSLQIQRGEYVAIMGPSGSGKSTLMNLIGCLDTPT
                     KGGYLLNNKQVGQMNDNELARIRNEEIGFVFQTFNLLPRASALQNVELPLVYAGVPGK
                     ERSTRAKQALEKVELADRMGHRPNELSGGQRQRVAIARALVNNPSILLADEPTGNLDS
                     KTGAEIMALFARLHESGNTIVLVTHEPDIAAHAHRSIHLRDGQIEKDVVRAA"
     gene            complement(642..1520)
                     /locus_tag="AC16CY_06950"
     CDS             complement(642..1520)
                     /locus_tag="AC16CY_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353557.1"
                     /translation="MALLEVRHLVKHFSRPGGMFRQPSVVRAVDDVSFDIEQGDMFGL
                     VGESGSGKSTTGRCILRLLEPTSGEVLFRGESVLKFSKNRLRLARRDMQVVFQDPYSS
                     LNPRMRAGDIVEEPLVIHGLGTREERRTRVAELFELVGLETVHLTRYPHEFSGGQRQR
                     IAIARALALNPALIVADEAVSALDVSIQAQVVKLLVDLQRRMGLTYLFIAHDLRLVEH
                     ICNRVAVMYLGKIVEIGETKALFRNAAHPYTRALLSAVPALDPDAVRQRIEFDQASFV
                     RATPLREVEIGHWAAV"
     gene            complement(1520..2311)
                     /locus_tag="AC16CY_06955"
     CDS             complement(1520..2311)
                     /locus_tag="AC16CY_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3353558.1"
                     /translation="MPPLLDIQRLTVRFEKPGSVVTAVDEVSLTIDAGETLGLVGESG
                     SGKSVTALAILRLLQPPGRITSGRIVFEGRDLLRLSEPEMRAVRGARISLIFQEPATA
                     LNPTMSVGAQIAEALTVHGAGRAEARRQAIELLAAVRIPDAERRARDYPHQLSGGMRQ
                     RVMIAIAIACRPALVVADEPTTALDVTVQAQVLDLLQELKTRYGLALLLITHDFGVIA
                     EMADRVVVMHQGRAVEQGPVRQVLRDPADDYTRRLLAAVPGRGQR"
     gene            complement(2316..>2918)
                     /locus_tag="AC16CY_06960"
     CDS             complement(2316..>2918)
                     /locus_tag="AC16CY_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013781.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR3353559.1"
                     /translation="GAQSLHCNARDEALALPTEESATIALRTQQIILHESGVASTVDP
                     VGGAYAIEEWTDRLEREASQILERIASAGGTLAAIEGGLMQRDIQESAYRAQQDFDAG
                     KMIVIGVNRYTDAERGSGSVFRLDASVEEAQIARVRGVRASRSASLCQEAIDAVERAA
                     RGGDNLMPAVIDAVDRHATLGEIADAMRRVFGEYHDATSA"
BASE COUNT          525 a          960 c          911 g          522 t
ORIGIN      
        1 gctccgagga gatacatgcg ctccgcggcg tctcccttca gatacagcgg ggagagtatg
       61 tcgccatcat gggaccgtcc gggtccggga agtcgaccct gatgaacctg atcggctgcc
      121 tggatacacc gaccaaaggt ggctacctgc tgaacaacaa gcaggttggc cagatgaacg
      181 acaacgagct ggcgcgcatt cgcaacgagg agattggatt cgttttccag acctttaact
      241 tgcttcctcg ggcatccgcc cttcagaatg tggagctgcc gctcgtctac gccggagtac
      301 ctggtaaaga acgatctact cgagccaagc aagcccttga aaaagttgaa ctagccgatc
      361 gaatgggcca tcgccctaat gagctgtctg gtggtcagcg ccagcgcgtt gcgatcgcgc
      421 gagccctcgt caacaatccc tcgatattgc tggcggacga gcccacaggg aacctcgatt
      481 cgaagacagg tgccgagatc atggccttgt ttgcccgtct tcacgagtcg ggcaacacca
      541 tcgtgttggt gacgcatgaa cccgacatcg ccgcccacgc ccataggtcg attcacctac
      601 gcgatgggca gattgaaaag gatgtggttc gggcagcgta atcagacggc cgcccaatgc
      661 ccgatttcga cctcgcgcag cggtgtcgca cggacgaaag acgcctggtc gaattcgatt
      721 cgctgacgga cggcatcagg gtcaagggct ggaacagcgc tcaggagcgc cctggtatac
      781 ggatgggccg cgttccgaaa cagggccttc gtctcgccta tctctacaat ctttcccagg
      841 tacatgacgg cgacgcggtt gcagatgtgc tcgacgagcc gcaggtcgtg agcgatgaac
      901 aagtacgtca gccccatccg gcgctgcaga tccaccagga gcttcaccac ctgcgcctga
      961 attgacacgt ccagcgccga cacagcctcg tcggcgacga tgagcgcagg gttgagcgcc
     1021 aacgcccggg caatcgcgat tcgctggcgt tgtccgccgc tgaattcgtg ggggtaccgc
     1081 gtcaggtgga ccgtctcgag cccgacgagc tcgaagagct ccgccaccct cgttcgacgc
     1141 tcttctctcg tgcccaaccc gtgaatcacg agcggttcct cgacgatgtc cccagcccgc
     1201 attcgcggat tgagcgagga gtaggggtcc tgaaacacga cctgcatgtc gcgccgggca
     1261 agccggagac ggttcttcga aaacttcagg acgctctcgc cccggaagag cacctcacca
     1321 ctggttggtt cgagcagtcg cagaatgcag cgccccgtcg tgctcttgcc gctgcccgac
     1381 tcgccgacga gcccgaacat gtcaccctgt tcgatgtcga agctgacgtc gtccacggca
     1441 cggacgacgg acggctgacg gaacatgccg ccgggccgcg aaaagtgttt gaccaggtgc
     1501 cggacttcga gcaaggccat cagcgctggc cccggcctgg caccgccgcc agcagtcggc
     1561 gcgtgtagtc gtcggcggga tcacgcagga cctggcgcac gggcccttgt tcgaccgccc
     1621 ggccctggtg catcacgacg acccgatcgg ccatttcggc gatgacgccg aaatcgtgtg
     1681 tgatgaggag cagcgccagc ccgtagcgcg tcttgagctc ctgcagcagg tcgagcacct
     1741 gcgcctggac ggtgacgtcg agcgccgttg tcggctcgtc cgcgacgacc agtgcggggc
     1801 ggcatgcgat cgcgatggct atcatcacgc gttggcgcat tccgccggac aattgatggg
     1861 ggtagtcgcg ggcccgtcgt tccgcatcgg ggattcgcac cgcggcgagc agttcaatgg
     1921 cttggcgccg cgcctccgcc ctgccggctc cgtgtaccgt cagcgcttcg gcgatctggg
     1981 ccccgacgct catcgtcggg ttgagcgccg tcgcgggctc ctgaaagatg agcgagattc
     2041 tggcgccgcg aacggcgcgc atttcgggtt ccgacaaccg aagcaagtcg cggccttcga
     2101 acacgatgcg tccgctggtg atgcgcccgg gcggctgcag cagcctgaga atcgccaacg
     2161 ccgttacgga cttgccgctg ccggattcgc cgaccaggcc gagcgtctcc cccgcgtcga
     2221 tcgtcaggct cacctcgtcc accgcggtca ccacggaccc gggcttctcg aacctgacgg
     2281 tcaaccgctg aatatcgagg aggggcggca tcggctcagg cgctcgtggc gtcgtgatac
     2341 tccccgaaca cgcggcgcat cgcgtcggcg atctcaccga gcgtcgcgtg ccggtcaaca
     2401 gcgtcgatga ccgccggcat gaggttgtcg cccccgcgcg ccgcccgttc gaccgcgtcg
     2461 attgcctcct gacagaggga agcgctcctg ctggctcgaa ccccgcggac gcgggcaatt
     2521 tgtgcttctt cgacggaagc gtcgagacga aacacgctgc ccgaaccgcg ttccgcatcg
     2581 gtgtatcggt tgacgccgat cacgatcatc tttccggcgt caaagtcctg ctgcgcccgg
     2641 taagccgatt cctgaatgtc ccgctgcatg agcccgcctt cgatggccgc aagcgtcccc
     2701 ccagccgagg cgattcgctc gaggatctga gaggcctcgc gctcgagacg gtcggtccac
     2761 tcctcgattg cgtatgcacc gcccacagga tcaaccgtgg acgcgacccc gctctcgtgg
     2821 aggatgattt gttgcgtacg gagagcaatc gtggcgctct cctccgtcgg gagcgccagt
     2881 gcctcgtccc tggcattgca gtgaagggac tgcgctcc
//
LOCUS       DBNGSH010000298         2222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2570638, whole genome shotgun sequence.
ACCESSION   DBNGSH010000298 DBNGSH010000000
VERSION     DBNGSH010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2570638"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="AC16CY_06965"
     CDS             <1..736
                     /locus_tag="AC16CY_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353560.1"
                     /translation="SVLGLDPFRDAYKLQERIGVQLQQAQLQKRIKVWEAVHLWAALY
                     RRKRSDGDRLVEQLGLADKRNAWFMTLSGGQKQRLFIALALIHDPDVVMLDELTTGLD
                     PQARRAIWEMVRGIRDRGKTVLLTTHLMEEAERLCDRIAIIERGRVIDIGTPAGLVRR
                     HCPEHSVVVATTNGDAESWFRAIPGVDSVTRHDGRLKIAGRDESLVTRVIECVSEHRI
                     RVTEFRTELPNLEDVFLKLTGHSIRE"
     gene            766..1512
                     /locus_tag="AC16CY_06970"
     CDS             766..1512
                     /locus_tag="AC16CY_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353561.1"
                     /translation="MRGLINLTWLEIKIFVREPMGVFGTIGIPVLMFLVLSRLAGPDI
                     ARVAARPGGAVLAALPALAGIMMALSAVTSLVTIISIYREGGILKRLRATPLSPVVIL
                     SAHVIVKLVFTAITLAVLMIAGRRYFPPGTDVPVLQFGAALLFTTWAILSIGFLVASI
                     VPTARFAQPLAALIFYPLLAFSGLFFPIALLPPWLEAIASVQPLTHAVSLLDGIWRGQ
                     GWTAHLGDVTALALTFLVFTAISSRVFRWE"
     gene            1663..>2222
                     /locus_tag="AC16CY_06975"
     CDS             1663..>2222
                     /locus_tag="AC16CY_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353562.1"
                     /translation="MASSASALRPAQIAVDRPRTFIKSESFDRALLVLPLVTGLAAAS
                     VALIDIRLFPLLLLADLWLLGYHHVVATYTRIAFTTDALRQNRFMAVDLLLIVTAVTL
                     GLAFTAGAWVVATSFLYLQWFHYMRQGYGIGRMYYRATPEGREAGARDLTADLVIYLV
                     PIYGIAARSATMGDTFLALPVKTVVLP"
BASE COUNT          341 a          764 c          706 g          411 t
ORIGIN      
        1 ctcggtgctt ggcctcgatc ctttccgcga tgcctacaag cttcaggaac gcatcggcgt
       61 gcagcttcag caggcgcagc tccagaaacg catcaaggtc tgggaggcgg tgcatctgtg
      121 ggccgccctc taccgccgaa agcgctcaga cggcgaccgc ctggtcgagc agctcgggct
      181 ggccgacaag cgcaacgcct ggttcatgac gctctcgggc ggccagaagc agcggctctt
      241 catcgcgctc gcgctgatcc acgatccgga cgtcgtgatg ctcgacgagc tgaccacggg
      301 cctcgacccg caggcccgcc gcgcaatctg ggagatggtc cgtggcatcc gcgatcgcgg
      361 aaaaacagtg ctgctgacga ctcacctcat ggaagaggcc gagcgcctct gcgaccgcat
      421 cgccatcatc gagcgcggcc gcgtcatcga catcggcacg cccgccggcc tcgtccggcg
      481 ccactgtccc gaacacagcg tcgtggttgc gacgacgaat ggcgacgccg agtcgtggtt
      541 ccgcgcgatt ccaggcgtgg attcggtcac tcgccacgat ggccgcctca agattgccgg
      601 ccgcgacgag agcctcgtga cgcgagtcat cgagtgcgtg tccgagcatc gcatccgtgt
      661 gacggagttc cgcacggagc tgcccaatct cgaggacgtt ttcctgaagc tgacaggtca
      721 ctcgatcagg gagtaggggg cagggggcag ggggcaggga agagcatgcg cggactcatc
      781 aacctgacgt ggctcgagat caagatcttc gtgcgggagc cgatgggcgt gttcgggacc
      841 atcggcatcc ccgtgctgat gttcctggta ctgagccgcc tcgcgggccc cgacatcgcg
      901 cgagtggcgg cgcgccccgg cggcgccgtg ctcgcagcgc tgcccgcgct ggccgggatc
      961 atgatggcgc tgagcgcggt gacctcgctc gtgaccatca tctcgatcta ccgcgaggga
     1021 ggcatcctca agcgtctccg ggccacgccg ctttcaccag tcgtgattct ctcggcgcac
     1081 gtcatcgtca agctggtgtt cacggcgatc acgctcgccg tcctgatgat tgccggccgg
     1141 cggtacttcc cgcctggtac cgacgtgccc gtgctgcagt tcggcgcggc gctcctgttc
     1201 acgacgtggg cgattctctc gatcggcttc ctcgtggcca gcatcgttcc cacggcgcgc
     1261 ttcgcgcagc cgctggcagc gctgatcttc tacccgctgc tcgcgttctc gggcttgttc
     1321 ttcccgatcg ccctcctgcc tccgtggctc gaggcgatcg ccagtgtcca gccgctcacc
     1381 cacgccgtgt cgctgctcga cgggatctgg cgcggacagg ggtggaccgc ccatcttgga
     1441 gacgtgacgg ccctggcgtt gaccttcctc gttttcacgg caatctcgag ccgcgtcttt
     1501 cggtgggaat aagggagaga cacgtaactc aaaactcaaa acgcaaaact caaaagcgta
     1561 atctcgcttg caagtctggg cgtggaccga tccgcgtctg cacgacggtt cacggcactg
     1621 cagccgacac ccgagtactg agcttgcacc cgaccagcct ttatggcctc gtctgccagc
     1681 gcactccggc ccgcacagat cgccgtcgat cggccgcgca cgttcatcaa gagcgagtcg
     1741 ttcgaccggg cgctgctggt tctgccgctc gtcacgggat tggccgccgc gagtgtcgcg
     1801 ctgatcgaca tccgcctctt cccgctgctg ctccttgcgg atctgtggct gctcggctac
     1861 caccacgtcg ttgccacgta cacgcggatc gccttcacca ccgacgcgct gcgccagaac
     1921 cgcttcatgg cggtcgatct gctgctgatc gtgacggcgg tcacgctcgg gctcgcgttc
     1981 acggcgggcg catgggtggt cgccacatcg ttcctgtacc tccagtggtt tcactacatg
     2041 cgccagggct acggcatcgg gcgcatgtac taccgggcga cgcccgaggg acgggaggcc
     2101 ggggcccgcg acctgacggc cgacctcgtg atctacctcg tgcccatcta cggcatcgcc
     2161 gcccggtcgg cgacgatggg ggacacgttc ctcgcgcttc cggtgaagac ggtcgtcctg
     2221 cc
//
LOCUS       DBNGSH010000299         1728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_703623, whole genome shotgun sequence.
ACCESSION   DBNGSH010000299 DBNGSH010000000
VERSION     DBNGSH010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_703623"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="AC16CY_06980"
     CDS             complement(<1..1001)
                     /locus_tag="AC16CY_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3353563.1"
                     /translation="MSVILDTLRRARKGAGDERSSEPSGETTVRPVPAGLGLSAPRHP
                     QPPRRWWVTLAGVAVIVALGAWLALRFISSREVASVAAPPPTAVVQAPRTGAQTPAAP
                     PDTEPVQPVLRARNPEPGTPSPAPGAQSPEPKVQSREPRAPSPAPNAQSPGPRVDNFE
                     LAVRHHRLGDFDQARRHYLAVLGEDEFHVEARNNLGLLYHERGMENDAIEQFRRAIGI
                     NPRYNTARSNIAVALTSAGRLAEARAELRAALALEPRSADLLVNMALVEKADRRPEQA
                     IELLLRAVGAEPVHAAAHYNLAVLYEERESFALAYDHYGAFLTYAGPEHRDRLGDVQR
                     "
     gene            complement(1015..>1728)
                     /locus_tag="AC16CY_06985"
     CDS             complement(1015..>1728)
                     /locus_tag="AC16CY_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR3353564.1"
                     /translation="LCRALLEKTDRHTFTALLLNPFLSEEDLLRGILQDLGVVSRGEE
                     RLGGRQPSKQDLVNTLYDFLLSIAPLGARAVLIIDEAQNLPLPILEQIRILSNLETDK
                     EKLLQIILVGQLNLLPLLRSPELRQLDQRISIRYQLRPLTEEEVGAYVAHRLAIAGGT
                     RSVVFTPAALRLVWECSNGIPRLVNMLCDRALLGGYSARTARIGDEIVATAAEGLDLK
                     PAARSQRSVIGRLLGRPGS"
BASE COUNT          264 a          579 c          600 g          285 t
ORIGIN      
        1 cgctgcacgt cgccgagacg gtcgcgatgt tccgggccgg cgtacgtcag gaaggccccg
       61 tagtggtcgt acgccagggc gaacgactcc cgttcttcgt agagcacggc aaggttgtag
      121 tgtgccgcgg cgtgcacggg ctcggcgcca acggcgcgga gaagaagttc gatggcctgc
      181 tccggtcggc gatcggcctt ctcgacgagc gccatgttca cgagcaggtc tgcactgcgc
      241 ggctcgaggg caagcgccgc gcgcagttct gcccgagcct cagcaagccg gcccgcactc
      301 gtcagcgcga ccgcgatgtt gctccgtgcc gtgttgtacc gcgggttgat gccgatcgcg
      361 cgccggaact gctcgatcgc gtcgttctcc atcccgcgct cgtggtacag cagaccgagg
      421 ttgttgcgcg cctcgacgtg gaactcgtcc tcgccgagca cggcgagata gtgtcgtcgc
      481 gcctgatcga agtcgccgag acgatggtgc cggacggcaa gctcgaagtt gtcgacgcgt
      541 ggccctgggc tctgggcgtt cggagccggg ctcggggctc tgggctctcg gctctggact
      601 ttgggttctg ggctctgggc tccaggcgcc gggctgggcg ttccaggctc cgggttccgg
      661 gctcgcagca ccggctggac gggctccgtg tccggtgggg ccgctggcgt ctgcgccccc
      721 gtgcgaggcg cctggacaac agccgtggga ggtggggccg cgaccgatgc gacctctcga
      781 cttgaaatga atcgaagcgc cagccacgcg cccagtgcga cgattacggc gacaccggcc
      841 aacgtcaccc accatcgacg cgggggctgc gggtgacgcg gcgccgacag gccgaggccc
      901 gctggcaccg ggcgcacggt ggtctcgccg ctcggctcgg acgaccgctc gtcgccggca
      961 cccttgcgcg cccgacgcag cgtgtcgagg atgacgctca tgaggcttcc gctgtcacga
     1021 gcccggcctt ccaagcaacc ggccgatcac ggatcgctgc gaccgggcag ccggtttcag
     1081 atcgagccct tcggccgcgg tggccacgat ttcgtcgcca atccgtgcgg tccgggccga
     1141 gtagccgccc aacaacgcgc gatcgcacag catgttgacg agtcgaggaa tgccgttcga
     1201 gcattcccac acgagccgca gcgccgccgg cgtgaacacc acgcttcgag ttccgcccgc
     1261 aatcgccagc ctgtgcgcca cgtacgcgcc gacctcctcc tcggtcagcg gcctgagctg
     1321 gtaccggatc gagatgcgct gatcgagctg acgaagctcc ggcgatcgca gcagcggcag
     1381 caggttgagc tgcccgacga ggatgatctg cagcagcttt tccttgtcgg tctcgaggtt
     1441 cgacagaatg cggatctgct ccagtatggg cagcggcaga ttctgggcct catcgatgat
     1501 gagcacggcg cgcgcgccga gaggagcgat ggacagcagg aagtcgtaca gggtgttgac
     1561 gagatcctgc ttgctcggtt gtcggccccc caggcgctcc tcgcctctcg agaccacgcc
     1621 gagatcctgc aggatccccc gcagcagatc ctcctccgac aggaacgggt tgaggagcag
     1681 cgccgtgaac gtgtgccgat ccgtcttctc gagcagagcg cggcagag
//
LOCUS       DBNGSH010000300         7608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4268789, whole genome shotgun sequence.
ACCESSION   DBNGSH010000300 DBNGSH010000000
VERSION     DBNGSH010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4268789"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="AC16CY_06990"
     CDS             complement(<1..1143)
                     /locus_tag="AC16CY_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3353565.1"
                     /translation="MRTVHHMALRFHALSSLNDSRPHFLALAAGFALLLSSAQTARQQ
                     VPIFEPDPLWSEALPNKWVNGQVGGVAVDSHDNVWVFHRPATIPDGEKAASLNPPQAE
                     CCIPAPPVLQFNAAGKFLRAWGGPGPGYDWFSTEHGIFVDGDDNVWLSGSAREDNHIL
                     KFTSTGTFVMQIGRPGRNKGSHDTGNLGGPAGLFVHRGELFVADGYFNHRVIVFDART
                     GAYKRHWGAYGKAPDDTYAFPARAQLLQAQPAPQFHNPVHAVIVSNDDLVYAADRTNN
                     RLQVFRLDGTFVKETFVARNTLQAEGTVHHFALSPDKAQQFLYVVDGSNKAVRVLNRQ
                     TLEIVSSVGGHAGHNAREFFHIHSAAVDSKGNLFLGEVNNGQRYYRY"
     gene            complement(1164..2915)
                     /locus_tag="AC16CY_06995"
     CDS             complement(1164..2915)
                     /locus_tag="AC16CY_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3353566.1"
                     /translation="MRIHSHRPAVAACALALAASAITLSGQYSLTVNQDRLINAANEP
                     QNWLLMNGDYGSLRYSKLTEINRENVKNLRMVWALALGGMQDVGQNGPENETNPLIDN
                     GFMYTSDGWGTIYKIDARNPGRGQFAWVADPGVPHEGNTVRTRGIALWEDKVIANLPD
                     GRVIAINRDNGEIVWDKKVTAKNEFGNQERFLTAPLVAGGKVLVQNGAGDGGTRGWVA
                     ALDVNTGNELWRWYTVPRPGEPGSETWKDDHNAWKTGGGGIWQTGSYDAAQNLYIFGT
                     GNPFPIYDPQFRPGDNLFTNSVVALDVATGKMKWYFQYTPNDSWDFDEAGVFMLYDTT
                     INGRARKVVGHFARNGFFYSLDRTTGRFIAAEKYVNHLNWTRGIDPETGKPMDYNPKL
                     DVQTYNPEARALRGDPKKRSCPTWHGGVAHQPMAFNPVKNIAYGVGTEGCFTQNGAAV
                     ASLSKDGGIDTKNSQQRQYSSDLYYGALTAFDTVTHKLVAKAVTDIEIRSGALATAGG
                     LVFTALQDGWVVAYNDETLQELWRFNVGTPLKGAPVTYAIGPRQYLAVQASGRHLHPV
                     NWDKLQNSSYLFVFALN"
     gene            complement(3018..3422)
                     /locus_tag="AC16CY_07000"
     CDS             complement(3018..3422)
                     /locus_tag="AC16CY_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019502253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3353567.1"
                     /translation="MVGTLVDSNVILDVLTENEEWWDWSSSRLAESASDGMLVINPII
                     YAEVSARFERIEELDEALPPDYYRRVPLPWEAAFLAGQCFVKYRRRGGSRRSPMPDFY
                     IGGHAAIAGLTLLTRDARRYRAYFPTLRIVAP"
     gene            complement(3424..3654)
                     /locus_tag="AC16CY_07005"
     CDS             complement(3424..3654)
                     /locus_tag="AC16CY_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3353568.1"
                     /translation="MRITSKGQVTIPLPMRQQLGLLPNTEVEFELDRDAVRVKRAAGA
                     RRRGRGPALVDHMRGRGTRRITTDEIMKLTRG"
     gene            3919..4476
                     /locus_tag="AC16CY_07010"
     CDS             3919..4476
                     /locus_tag="AC16CY_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353569.1"
                     /translation="MPVKKSIRRKKASPMSKAALIKQYERLTSGMDKSAVAAVMAIGA
                     MAAAMLIAMAWPSRPAAAVPENEIVAASTASGFDEPYSAAGAAEAREPQSQVVTITGC
                     LERDDETFRLKDTTGVDAPRSRSWKSGFLRKRPASVEVIDASNRLRLNSHVGERISVT
                     GTLEDKELQVRSLRRVASSCASDNA"
     gene            4581..6614
                     /locus_tag="AC16CY_07015"
     CDS             4581..6614
                     /locus_tag="AC16CY_07015"
                     /inference="COORDINATES: protein motif:HMM:NF022113.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3353570.1"
                     /translation="MRIRLMRSAAAVSLALGLAHAGFIVGAAQSPSISSWTPESVMSV
                     KRVSAVTPSPDGTRVAFVVGEAVMQGDKSEWLSQIHVANADGSDSRQLTRAEKSASAP
                     RWSPDGTWIGFLTSRSGSGNVWRIPVAGGEAEQLTSEKADVASFDWSPDGTQIAFVMT
                     DPKSDEEETAAKEKRDWRTIDDNVKMRRLYVLPVERDAKGGRPSRKLTTASYSIGDFD
                     WSPDGRTIVFEHQPTPVANDWARADISIVSVTDGAVRPLINSPQPEQTPRFSPDGTRV
                     AFVGVEPNFIWATSARVHVVPASGGSPHALSATFDMQPAIVDWTADGTRIIASEVNRT
                     VPMVYAIPVDGKAAVAVSPAKTHVNGASMNARRTHLGFVSQDFDKAPEAFVAKVDGAF
                     TPVQASHVQPAIQAAIGTSEVITWTSSDGQAVEGILTYPARYQRGTRVPLLLIVHGGP
                     TGVFVNNFTGIANQYPVALFASQGYAVLRVNPRGSSGYGKAFRFANQADWGGGDYRDL
                     MTGVDRAIEMGVADPDRLGVMGWSYGGYMTSWIVTQTKRFKAASAGAAVTNLISFNGT
                     SDIPDFLPDYFKGEFWKAFESWRAHSPVLNANGVTTPTLIQHGEADVRVPISQSYEFY
                     TALKRQGTTTKLTVYPRQGHGFTEPKMVLDVARANLEWFDRFVMGKAATSTRR"
     gene            6611..>7608
                     /locus_tag="AC16CY_07020"
     CDS             6611..>7608
                     /locus_tag="AC16CY_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353571.1"
                     /translation="MTGRLRFPCALAAPIVICALWPSAVSGQSATKPKASQADTARPY
                     APPRTPWGDPDLQGTYTNKYEQSTPLERPPRFEGRRAEDVSGTELADILATRNRQVLE
                     RAAGVGPLQFRDTLDVTRGNRAWLIVDPPDGRIPPLTAAARTRIGPPDPFLETGIQGI
                     VNARQRTRSSFDGDGTFESVADLSLWDRCITRGLPGAMMPHILGNSYEIVQAPGVVAI
                     RYELVHDARVIPLDRRPHAARAIELDMGDPRGHWEGSTLVVETTNFKDRSTYRNAIAA
                     TLRLVERFTRTAPGRIEWTVTVSDPSTWTKPWTFSMPLTMNDAEPVLEFACHEGNYA"
BASE COUNT         1237 a         2497 c         2567 g         1307 t
ORIGIN      
        1 gtaccgatag tagcgctgcc cgttgttcac ttcgccgagg aagaggttgc ccttcgaatc
       61 caccgcggcg ctgtggatgt ggaagaactc gcgtgcgttg tgcccggcgt ggccgccgac
      121 gctggacacg atctccaggg tctggcggtt cagcacccgg acggccttgt tcgagccgtc
      181 gacgacgtag agaaactgct gggccttgtc gggcgacagc gcgaagtgat ggaccgtgcc
      241 ttcggcctgc agcgtgttcc gcgcgacgaa ggtttccttg acgaacgtgc catcgagcct
      301 gaatacctgg agccggttgt tggtgcgatc ggcggcgtag acgagatcgt cgttcgacac
      361 gatcaccgcg tgcacggggt tgtggaactg cggggcgggc tgcgcctgca ggagctgcgc
      421 gcgcgccgga aacgcgtagg tgtcgtccgg cgccttgccg tacgcgcccc agtgccgctt
      481 gtaggcaccg gttcgtgcgt cgaacacgat gacgcggtgg ttgaagtagc cgtcggcgac
      541 gaagagctct ccgcggtgca cgaagagacc ggccggtccc ccgaggttcc cggtgtcgtg
      601 gctgcccttg ttgcggccgg gtcggccgat ctgcatcacg aatgtcccgg tgctcgtgaa
      661 cttgaggatg tggttgtctt cccgcgcgct cccgctcagc cagacgttgt cgtccccgtc
      721 gacgaagatg ccgtgctcgg tgctgaacca gtcgtacccc gggccggggc cgccccacgc
      781 gcgaaggaac ttgcccgcgg cgttgaattg cagcaccggc ggggcaggga tgcagcattc
      841 ggcctggggc ggattcagcg acgccgcttt ctcgccgtcg ggaatcgtgg ccggacggtg
      901 gaacacccac acgttgtcgt gtgagtccac ggccacgccg ccgacctggc cgttgaccca
      961 cttgttgggg agcgcctcgg accacagcgg atccggctcg aagatcggca cctgctgccg
     1021 cgcggtctgc gcgctcgaga ggagcagcgc aaaccccgcc gccagcgcaa gaaaatgggg
     1081 tcgggagtca tttaacgagg acagcgcgtg gaaacgaagc gccatgtgat gcactgtcct
     1141 cattaaatga ctcccgaccc catttaattc aacgcgaaga cgaacaggta gctcgagttc
     1201 tgcagcttgt cccagttcac cgggtgcagg tggcggccgc tggcctgcac ggcgaggtac
     1261 tgcctcgggc cgatggcgta ggtgacgggc gcgcccttga gcggcgtgcc gacgttgaag
     1321 cgccacagct cctgcagcgt ctcgtcgttg taggcgacga cccagccgtc ctggagcgcc
     1381 gtgaagacca ggccgcccgc cgtcgcgagc gcccccgatc ggatctcgat gtcggtcacc
     1441 gccttggcga cgagcttgtg ggtgaccgtg tcgaacgccg tgagcgcccc gtagtagaga
     1501 tcgctgctgt actggcgctg ctggctgttc ttggtgtcga tgccgccgtc tttcgacagg
     1561 gacgcgaccg cggcgccgtt ctgcgtgaag cacccctcgg tcccgacgcc ataggcgatg
     1621 ttcttcaccg ggttgaacgc catcggctgg tgggcgacgc cgccgtgcca cgtggggcag
     1681 ctccgcttct tcgggtcgcc tcgcagcgcg cgcgcctccg ggttgtaggt ctggacgtcg
     1741 agcttcgggt tgtagtccat cggcttaccg gtctccggat cgatcccgcg cgtccagttc
     1801 aagtggttca cgtacttttc ggcggcgatg aaccggccgg tggtccgatc cagcgagtag
     1861 aagaagccgt tgcgcgcgaa gtgcccgacg accttgcgcg cccgcccgtt gatcgtcgtg
     1921 tcgtagagca tgaacacgcc cgcctcgtcg aagtcccacg agtcgttcgg cgtgtactgg
     1981 aagtaccact tcatcttgcc ggtcgccacg tcgagcgcga cgaccgagtt cgtgaagagg
     2041 ttgtcgcccg gacggaactg cgggtcgtag atcgggaacg ggttgcctgt gccgaagatg
     2101 tagaggttct gcgcggcgtc gtaggagccg gtctgccaga tgccgccgcc gcccgtcttc
     2161 cacgcgttgt gatcgtcctt ccacgtctcg ctgccgggct cacctggcct ggggaccgtg
     2221 taccagcgcc acagctcgtt gcccgtgttg acgtcgagcg cggccaccca tccgcgcgtg
     2281 ccgccgtcgc ccgcgccgtt ctggacgagg accttgccgc cggccacgag cggcgcggtg
     2341 aggaaccgct cctggttgcc gaactcgttc ttggccgtga ccttcttgtc ccagacgatc
     2401 tcgccgttgt cgcgattgat ggcgatcacg cggccgtccg gcaggttggc gatcaccttg
     2461 tcttcccaca gcgcgatgcc gcgcgtgcgc acggtattgc cttcgtgggg cacgccgggg
     2521 tcggccaccc aggcgaactg gccgcgtccg gggttgcgcg cgtcgatctt gtagatcgtg
     2581 ccccacccgt cgctcgtgta catgaacccg ttgtcgatga gcgggttcgt ctcgttctcg
     2641 gggccgttct gcccgacgtc ctgcatgccg ccgagcgcca gcgcccacac catccgcagg
     2701 ttcttcacgt tctcgcggtt gatctcggtc agcttcgagt agcggagcga gccgtagtcg
     2761 ccgttcatca gcagccagtt ctgcggctcg ttcgccgcgt tgatcaaccg gtcttggttg
     2821 accgtgaggg agtactggcc ggagagcgtg atcgcggaag ccgcgagcgc cagcgcgcag
     2881 gccgccacag ccggtcggtg tgaatggatg cgcatacgct tcgctccttg gaaggtgcgc
     2941 aaatactaca acatggcttc gtgggcccgg cacggggccg cccgccttcg ccgctctgcg
     3001 gcttcggcgc ggcaaggcta gggcgcgacg atgcgaaggg tcggaaagta ggcgcggtac
     3061 cgtctggcat cacgcgtcag cagcgtgagc cccgcgatcg cggcgtgacc gccgatgtag
     3121 aagtcgggca tcggcgatcg cctgctgccg ccgcggcgcc ggtatttcac gaagcactgc
     3181 ccggcgagga aggccgcctc ccatggcagc gggactcgcc ggtagtagtc ggggggcagc
     3241 gcctcgtcca gctcctcgat gcgctcgaac cgggccgaga cctcggcgta gatgatcggg
     3301 ttgatgacga gcatcccgtc gctggccgac tccgccagcc gcgacgacga ccagtcccac
     3361 cattcctcgt tctcggtgag gacgtcgagg atgacgttgc tgtccaccag cgtgccgacc
     3421 acgctaccct cgggtcagtt tcatgatctc gtcggtcgtg atgcggcgcg tgccccggcc
     3481 tcgcatgtga tcgacgaggg ccgggccgcg gccgcgccgg cgcgcgccgg cggcgcgctt
     3541 cacacggacg gcgtcgcggt cgagctcgaa ctccacctcg gtgttcggca gcaggccgag
     3601 ctgctggcgc atgggcaacg gaatcgtcac ctgccccttg ctggtgatcc gcatgtatta
     3661 ctcctaccag taagagtatt actcaacttg gcgcgtcaac ggccgcaccg cctttcacac
     3721 gtcttcacgc ccagggtcga aagtggggtc cggcgatcac ggatttagcc ttctgcccgc
     3781 gcgccgcggt cgccgggacc agcgagaact tccgcctaaa ggcctggctc gcgggctctt
     3841 agcgcgcgtc gtcgtcgtgc gggcacctcg tttgccttga ggaccgaggg tccaacgggt
     3901 ttcaccaagg aggacacgat gccggtgaag aaatcgatcc gccggaagaa ggcgtcgccg
     3961 atgagcaagg ccgcgctcat caagcagtac gagcgtctga cgagcggaat ggacaagtcg
     4021 gcggtcgcag ccgtaatggc aattggcgca atggcagcag cgatgctgat tgccatggcc
     4081 tggccgtcac ggccggcagc ggcggtgccg gagaacgaga tcgtcgcggc gtccacggcg
     4141 tccggcttcg acgagccgta cagcgccgcc ggggcagccg aggctcgcga gcctcagtcg
     4201 caggtggtga cgatcaccgg atgcctcgag cgcgacgacg agacgttccg gctgaaggac
     4261 acaactggcg tggacgcgcc tcggtcgaga agctggaagt cgggcttcct ccggaagcgc
     4321 ccggcgtcgg tcgaggtgat cgatgcgtcg aatcgcctca ggctgaacag ccacgtcggg
     4381 gaacgcatca gcgtcaccgg cacgctcgag gacaaggagc tgcaggtccg ttcgctgcgt
     4441 cgagtcgcct cgtcctgcgc gagcgacaac gcctgaccct cgctgctgcg ggctgctccg
     4501 gcagcccgca gccccaccct ttcttttctc gtccccgggg tagaattctc atcgcaccgg
     4561 cagtgtgtct ggaggctgcg atgaggattc gactgatgcg ttcggcggcc gcggtttctc
     4621 tcgccctcgg cctcgctcac gcgggtttca tcgtcggggc ggcgcagtcg ccctccattt
     4681 cgtcgtggac gcccgagtcg gtgatgagcg tcaagcgtgt gtctgccgtc acgccctcgc
     4741 cggacgggac gagagtcgcc ttcgtcgtgg gcgaagccgt catgcagggc gacaagagcg
     4801 aatggctctc ccagatccac gtcgcgaacg ccgacgggtc cgacagccgg cagctcactc
     4861 gggcagagaa gtcagccagc gctccgcgct ggtcgcccga cgggacgtgg atcggctttc
     4921 tcacgtcgcg cagcggcagc ggaaacgtct ggcgcattcc ggtcgcgggc ggcgaagcgg
     4981 agcagctgac gagtgaaaag gccgacgtcg cctccttcga ctggtcgcct gacggcacgc
     5041 agatcgcctt cgtgatgacc gacccgaaat cagacgaaga agagacggcg gcgaaggaga
     5101 aacgcgactg gcgcacgatc gacgacaacg tcaagatgcg gcgcctgtac gtgctgcccg
     5161 tggagcgcga tgcaaaaggc gggcgcccat cgaggaagct gacgacggcc agctactcga
     5221 tcggggactt cgactggtcg cccgacggcc gcacgattgt cttcgagcat cagcccacgc
     5281 ccgttgccaa cgactgggcg cgtgccgaca tctcgatcgt ctcggtaacc gacggggcgg
     5341 tgcggcccct gatcaattcc ccgcagccgg agcagacgcc gcgattctct cccgatggca
     5401 cgcgcgtcgc gttcgtcggg gtcgagccga atttcatctg ggccacgagc gcccgggtgc
     5461 acgtcgtccc tgcctccgga ggatcgccgc atgcgctctc ggccaccttc gacatgcagc
     5521 cggcgatcgt ggactggacg gctgatggca cgcgcatcat cgcgagcgaa gtgaaccgga
     5581 cggtgccgat ggtctatgcc atcccggtgg acggcaaggc ggccgtcgct gtgagcccgg
     5641 cgaagacgca cgtcaacggc gcgtcgatga acgcacgccg aacgcatctc gggttcgtgt
     5701 cgcaggattt cgacaaggcg ccggaagcgt tcgtggcgaa ggtggacggc gcgttcacgc
     5761 cagtgcaggc gagtcacgtg cagcccgcga ttcaggccgc gatcggaacg agcgaggtca
     5821 tcacctggac gtcgtccgat ggccaggctg tcgaaggcat tctcacctac ccggcgcgtt
     5881 accagcgcgg aacgcgcgtg ccgctgctgc tcatcgtgca cggcgggccc acgggcgttt
     5941 tcgtgaacaa cttcaccggc atcgccaacc agtacccggt cgccctgttc gcctctcagg
     6001 gctacgccgt tctccgcgtg aacccgcggg ggagcagcgg ctacgggaag gcgtttcgat
     6061 tcgcgaacca ggccgactgg ggcggcggtg actatcgcga tctcatgacg ggtgtcgatc
     6121 gcgcgatcga gatgggtgtg gccgatccgg atcggctcgg cgtcatgggg tggagctacg
     6181 gcggctacat gacgtcctgg atcgtcacgc agacgaagcg gttcaaggcg gcctcggctg
     6241 gagcggccgt cacgaatctc atcagcttca acggcacgag cgacatcccc gacttcctcc
     6301 ccgattactt caagggcgaa ttctggaaag cgttcgagag ctggcgtgcg cactcacccg
     6361 tgctgaacgc caacggcgtc acgacgccga cgctcataca gcacggcgag gccgacgtcc
     6421 gcgtgccgat ttcgcagagc tacgagttct atacggcgct gaaacggcag ggcacgacga
     6481 cgaagctcac ggtgtatccg cggcaggggc acggattcac cgaacccaaa atggttctcg
     6541 atgtagcccg ggcgaacctc gagtggttcg accgctttgt catggggaag gcggcgacga
     6601 gcacgcgcag atgaccggac ggcttcgctt cccgtgtgcg cttgcggcac cgatagtgat
     6661 ctgtgccctc tggccctcgg cggtcagtgg tcagagcgcc acgaagccga aggcgtcgca
     6721 ggcggacact gcgcgcccgt acgcgccgcc gcgcacgccc tggggtgacc ccgacctgca
     6781 aggaacctac acgaacaagt acgagcagag tactccgctc gagaggcctc cccggttcga
     6841 gggccggcgt gcagaagacg tgtcgggcac ggaattggcc gacatcctcg cgacgcggaa
     6901 caggcaggtt ctcgaacgcg cggccggtgt gggcccgctt cagtttcgcg acaccctcga
     6961 cgtcacccgg gggaatcgtg catggctcat cgtcgatccc cccgacggac ggattcctcc
     7021 gctcacggcc gcggcccgga cacgcatcgg gccccccgat ccgttcctcg agaccggcat
     7081 tcaaggcatc gtcaacgcca ggcagcgaac gcgcagcagc tttgacggtg acgggacgtt
     7141 cgagagcgtc gcagacctca gcctctggga ccgctgtatt acgcgcgggc ttccgggcgc
     7201 gatgatgccg cacatcctcg gcaactcgta cgagatcgtc caggcgcccg gagtcgtcgc
     7261 cattcgttac gagctcgtgc acgacgcccg ggtgatcccg ctcgatcgac ggccgcacgc
     7321 agccagggcc atcgagctgg acatgggcga cccgcgcggc cactgggaag gcagcacgct
     7381 ggtcgtcgag acgaccaact tcaaggaccg cagcacctac aggaacgcga ttgccgccac
     7441 cctgcgcctc gtcgaacgtt tcacgcgcac ggcgccgggc aggatcgagt ggaccgtcac
     7501 cgtgagcgac ccgtcgacct ggacgaagcc ctggacgttc tcgatgcctc tcacgatgaa
     7561 cgatgccgag ccggtcctcg aattcgcctg ccacgaaggc aactacgc
//
LOCUS       DBNGSH010000301         2000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2910901, whole genome shotgun sequence.
ACCESSION   DBNGSH010000301 DBNGSH010000000
VERSION     DBNGSH010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2910901"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC16CY_07025"
     CDS             complement(<1..226)
                     /locus_tag="AC16CY_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HIR3353572.1"
                     /translation="MVASEVHPFAKTGGLADVLGALPRALARLGHDVDVVMPRYRHVK
                     AGALIGQLRVVHGGQVDQVDLFAAVENRVRT"
     gene            550..1437
                     /locus_tag="AC16CY_07030"
     CDS             550..1437
                     /locus_tag="AC16CY_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HIR3353573.1"
                     /translation="MSEPGSRSTKGARPESREASELRAIRARHPELESAVDLHLELLE
                     LQRRVQSRLSLPWIPISPEVVQQRLAEAKPLLRFEDVSFEVSDLRLMVRQTADVLRRF
                     GALEEADFQKVQALGRDANLMDATARWYRKAFEQRAAPVGVPAATTPGATTDESAIDS
                     VLGLAMRPFLSRCAEVLQQRPELSQWTHPHCAMCGGEPDFAFITPSAERQLICGRCGL
                     RWKWKGLACPYCGNDNRDQITSFATPDGQYRLSGCDVCHRYLKAYDGRHASRPLMPVV
                     DAVATLPLDAVAMQRGYSS"
     gene            complement(1448..>2000)
                     /locus_tag="AC16CY_07035"
     CDS             complement(1448..>2000)
                     /locus_tag="AC16CY_07035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR3353574.1"
                     /translation="DLLHLALIASDNAAARALARVSSVGPDAFVERMNSKAAELGLTA
                     TSYTDPSGLYAANVSSAFDMARLITYVSGDERIAGVMRKQSYTITTGRRVINIHSTNQ
                     LVMNGDVDVQAGKTGFIRNSGYCLATLLRLPQGGPQVAVVVLGAKSNAGRFWETRHLF
                     NWIAGKAGSLISTPVEAATLPAD"
BASE COUNT          342 a          659 c          667 g          332 t
ORIGIN      
        1 ccgttcgaac gcgattctcg acggccgcaa acaggtccac ctggtccacc tgcccgccgt
       61 ggacgacccg gagctgtccg atgagggcgc cggccttgac gtgccggtac cggggcatga
      121 ccacgtcgac gtcgtggccg agccgcgcga gcgcgcgagg aagcgccccg agcacgtccg
      181 ccagtcctcc cgtcttggcg aaaggatgaa cctcggacgc gaccatcagg attcgcacac
      241 tactccacag gtcgcctgaa tcgagctcga gatctgtcgt tcacccttct ccgttctccc
      301 ttcttctgtt cgtgttcagg ttcgggggat catgcggccg gcgtagcgaa gagccccgga
      361 gggaacgccg aactccagca taccgaactg gcgaaccgaa cgtgcccgcc ttcgcgcgaa
      421 gcgcttcggc gaggtctcgc cgtagccctg gcggaggcgg aaacacgaac cgagaaggac
      481 agaaccgaga agcgtgaaca caggatctct tgctccacag gcagagccct tgactgctat
      541 catctcaccg tgagcgagcc aggcagtcga tcgacgaagg gcgcccgtcc cgagtcgaga
      601 gaggccagcg agttgcgcgc gatccgagcc cggcatcccg agctcgaatc cgcggtcgat
      661 ctgcatctgg agttgctgga attgcagcgc cgggtccaga gccgcctgtc cctgccgtgg
      721 atcccgatct ctccagaggt tgtgcagcag cgtctggccg aagcgaagcc gctccttcgt
      781 ttcgaggacg tgtcgttcga agtgagcgac ctgcgactca tggttcgtca gaccgccgac
      841 gtgctccggc gcttcggggc gctcgaggaa gccgacttcc agaaggtcca ggcgctcggc
      901 cgcgacgcga acctgatgga cgcgacggcg cgctggtacc gcaaggcctt cgagcagcgt
      961 gcggcccccg tcggcgtccc ggcagcgacg acgccgggag ccacgaccga cgagtccgcg
     1021 atcgacagcg tactgggact ggcgatgcgg ccgttcctgt cgcgttgcgc ggaagtgctg
     1081 cagcaacggc cggagctctc gcaatggacc cacccgcact gcgcgatgtg cgggggcgag
     1141 cccgactttg ccttcatcac gccatcggcc gagcggcagc tgatttgcgg ccggtgcggg
     1201 cttcgttgga agtggaaggg gctggcctgt ccctactgcg gcaacgacaa tcgcgatcag
     1261 atcacgtcgt ttgccacgcc cgacggccag tatcgtctct cgggatgcga cgtctgccat
     1321 cgctacctga aggcgtacga tggacggcac gcgtcacgtc ccctgatgcc ggtggtcgat
     1381 gccgtggcga cgctgccgct cgacgccgtg gcgatgcagc gaggctactc gtcgtagaga
     1441 agcagtctta gtcggccggc agcgttgccg cctcgactgg cgtgctgatg agcgagccgg
     1501 ccttcccggc gatccagttg aagaggtggc gcgtctccca gaagcggccc gcgttcgact
     1561 tggcgccaag cacgaccacg gcgacctggg gcccaccctg cggcagacgg aggagagtcg
     1621 cgagacagta gcccgagttc cggatgaagc cggtcttgcc cgcctgcacg tccacatcgc
     1681 cgttcatgac gagctggttg gtcgagtgga tgttgatgac acgtcgtccc gttgtgatcg
     1741 tgtaggactg cttgcgcatc acgcccgcga tgcgctcgtc ccccgacacg taggtgatga
     1801 gcctggccat gtcgaacgcg gacgagacgt tggccgcgta gagtcccgac ggatccgtgt
     1861 aggacgtggc cgtcagcccc aactcagccg ccttcgagtt catgcgctcg acgaatgcgt
     1921 cgggacccac gctcgacacg cgggccagcg ccctggcagc ggcgttgtcg gaggcgatga
     1981 gcgccaaatg cagcaggtcg
//
LOCUS       DBNGSH010000302         3804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4099778, whole genome shotgun sequence.
ACCESSION   DBNGSH010000302 DBNGSH010000000
VERSION     DBNGSH010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4099778"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC16CY_07040"
     CDS             <1..270
                     /locus_tag="AC16CY_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353575.1"
                     /translation="SSIARGVAGLVGVGTGIGLFLTVLLMLTLRASSGSADIGIGNMT
                     VYRPSIDPLALLAIAAVTALVGVAAAFVPARRAARMDPLVALRHE"
     gene            complement(477..842)
                     /locus_tag="AC16CY_07045"
     CDS             complement(477..842)
                     /locus_tag="AC16CY_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3353576.1"
                     /translation="MRLHRAMLFVKDLERMTAFYRDVLGLEPVEETRLDNWLEFKGEG
                     VRFALHAIPTTIAAGISIETPPRVRESGGTKFTFAVRDVDATLRTIEAMGLPLLERPW
                     GGIEAVDPEGNVFALERAA"
     gene            complement(912..1439)
                     /locus_tag="AC16CY_07050"
     CDS             complement(912..1439)
                     /locus_tag="AC16CY_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3353577.1"
                     /translation="MLMLTSRRYVIRALTGAFAALAAERPAFSQQLSATLPRPSPLPP
                     DQVRQFVQVAHSDLDATRKMLDSEHRLLRATWDWGGGDFETALGGAAHMGRRDIATLL
                     VDKGAPLDLFAAAMLGEIAIVRAALETRPALARVPGPHGIPLLAHAKAGGPPAMAVLE
                     YVSTVLARVEEKLHK"
     gene            1620..2417
                     /locus_tag="AC16CY_07055"
     CDS             1620..2417
                     /locus_tag="AC16CY_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3353578.1"
                     /translation="MDRYRFGEFEFDAGSGTLRRQGGGEGQRLPPQPARLLHVLAARG
                     GAIVTHDEIRRRLWPDTHVDFDTSLHFCVRQLRLALGDSAAEPRYIENVPRRGYRLVP
                     DVTRVDDAVVAAAARPGVRPWMHVALTIAIVAAIALSSYYVRPGAGSPRVRLAIMPLQ
                     APIAEWVLQDLSAIAGDSLGIVGPTTTSAYAGSDAGLQRLSADYGVDYILNGRDLSSN
                     GRPRLLAELIRVSDGAHVWVRPYEDLSDGRRIGQEISRHVARVLELR"
     gene            2461..2742
                     /locus_tag="AC16CY_07060"
     CDS             2461..2742
                     /locus_tag="AC16CY_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353579.1"
                     /translation="MFAAIGCATFHTPALQTGPSGNPLFHHVPVRLFSDLLMHDIGTG
                     DDIAQSVVAPEEIRTPAIAAVTALVGVAAAFVPARRAAHMDPLAALRHE"
     gene            2914..3333
                     /locus_tag="AC16CY_07065"
     CDS             2914..3333
                     /locus_tag="AC16CY_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353580.1"
                     /translation="MRYVTSTIAACVLASAAGVFAQEQAPPQPQPPAAREPAPAALEE
                     PAPKATLTGCVGQAKTTDGGTAYVLNKAEGGSATMYVLGGPSRSEVSANVNKKVEVTG
                     PVLEPAGANSDSAADAKVLRPPVMLVEAVKVVAQSCT"
     gene            complement(3487..>3804)
                     /locus_tag="AC16CY_07070"
     CDS             complement(3487..>3804)
                     /locus_tag="AC16CY_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3353581.1"
                     /translation="QRVLEEAGGVTSAARYVVIDTYDRWYEGYDLFEVAHPQTILAYG
                     LNGRDLPRGNGAPLRLRVERQCGYKNLKFLKSIRAVDSMAGIGRGTGGINSDIGFHWF
                     AGV"
BASE COUNT          644 a         1255 c         1261 g          644 t
ORIGIN      
        1 tcgagcatcg cgcgaggggt cgcgggtctc gtcggggtcg ggaccggcat cgggctcttc
       61 ctcacggtgc tgctgatgct cacattgcgc gcgagctcgg gatcagcgga catcgggatc
      121 ggcaacatga cggtgtatcg cccgagcatc gatccgctgg cgctgctggc catcgccgcg
      181 gtgaccgccc tcgtcggcgt cgccgctgcg ttcgtgccag cgcgccgggc cgcccgcatg
      241 gatccgctcg tggcactgcg ccacgagtga gtgcgcgtcg gcgacatcgc gccgttcctt
      301 cccggttccc ctcgtcgacg ccacggccca cactcggcac gagcgcctcc tgataacgct
      361 gaaagagcgt gcgttcgtct accttggacg ataggacgca ggaagggcct ctgatgtcgc
      421 tgagatcgag cacgtcgcca ccctcgaacc cgaactgacg ccgcggaggg ccaccttcag
      481 gcggcccgct cgagggcgaa cacatttcct tcgggatcca cggcctcgat gccgccccat
      541 ggccgctcga gcaacggcag gcccatggct tcgatggtcc gaagagtcgc gtcgacatcg
      601 cgcacggcaa aagtgaactt ggtgccacca ctctcccgca cgcgcggcgg cgtctcgatc
      661 gagatgcctg ccgcgatcgt agtgggtatc gcgtgcaggg cgaatcggac gccctcgccc
      721 ttgaactcga gccagttatc gaggcgggtc tcctcgaccg gctcgagtcc gagcacgtcg
      781 cgatagaacg ccgtcatgcg ttcgagatct ttcacgaaga gcatcgctct gtgtaatcgc
      841 attggttgtg gcccaaatcc tccgtgccct tcgtggcatc gtgacgcgct tttcagcacg
      901 ctgcgtgagt gttacttgtg cagcttctct tcgacccgcg ccagcacagt actgacgtat
      961 tcgagtacag ccatcgccgg cggaccaccc gctttggcat gcgcgagcag cggaatgccg
     1021 tgcggacctg gcacgcgggc gagcgccggt cgcgtctcga gcgccgcacg cacgatcgcg
     1081 atctctccga gcatcgccgc cgcgaacaga tcaagtggtg cccccttgtc gacgagcaga
     1141 gtagcgatgt cccggcgtcc catatgggca gcaccgccaa gcgcggtctc gaagtccccg
     1201 ccaccccagt cccaggtcgc acgaagcaga cggtgctcgg agtcgagcat ctttcgcgtg
     1261 gcgtcgaggt cgctgtgcgc cacttgaacg aactgccgca cctggtcggg cggaaggggg
     1321 ctcggccggg gcagagtcgc cgaaagctgt tgcgaaaacg ccggacgttc ggcggcgagt
     1381 gccgcgaagg cgccggtgag ggcacggatg acgtaacgac gcgaagtgag catgagcatg
     1441 acgttgcact cctggaaaag gccgagcgca gattcgccgg caacgcggcg ggagtcaacg
     1501 gagctgacgc tttggaaccc tgagcaaaca ctgagatccg tctgagaacg gccacttggc
     1561 tatccttccc atggcaacgg gaggcatact cgttcgcgag cgcgagagga cggcggttgg
     1621 tggatcgata ccggttcggt gagttcgagt tcgatgccgg aagtggcaca ctgcgccggc
     1681 agggcggcgg tgaagggcag cgtctgccgc cgcagccggc caggctgctt catgtgctcg
     1741 ccgctcgcgg tggtgcgatc gtgacgcacg acgagattcg ccggcggctc tggcctgaca
     1801 cccacgtcga cttcgatacg agcctgcatt tctgtgtccg gcagctccgc ctcgccctgg
     1861 gcgattcggc cgcggagccc cgctacatcg agaacgtgcc gcgccgcggc tatcgattgg
     1921 tgccggacgt gactcgtgtc gacgatgcgg tggttgctgc cgccgcgcgc cctggcgtca
     1981 ggccatggat gcacgtcgcg ctcacgatcg ccatcgtcgc cgcgatcgcg ctctcgtcgt
     2041 actacgttcg cccaggggcg ggatcgcctc gcgttcgtct tgcgatcatg ccgctccaag
     2101 cgcccatcgc cgaatgggtc ctccaggatc tgtccgccat cgccggcgac tcccttggga
     2161 tcgttgggcc gaccacgacg tctgcctacg cggggtccga tgcaggtttg caacgtcttt
     2221 ctgccgatta cggtgtcgac tacatcctca acggccgcga cctgagctcg aacggtcgtc
     2281 cgcggctgct cgcggagttg attcgcgtgt ctgacggcgc gcacgtgtgg gtacgaccgt
     2341 acgaggatct ctccgacggt cgccgaatcg gccaggagat cagccggcac gtggcgcgtg
     2401 tgttggaact gcgttagcag gcgcggccca atcgacgagg ccgcgcgcgc cggcgaacag
     2461 atgttcgcgg cgatcggctg cgcgacgttc catacgccgg cgctccagac cgggccgagc
     2521 ggcaatcccc tctttcatca cgtccccgtc cggctcttct ccgatctgct gatgcacgac
     2581 atcgggacgg gcgacgacat cgcacagagc gtcgtggcgc ccgaagagat tcgcacgcct
     2641 gccatcgccg cggtgaccgc actcgtcggc gtcgccgctg cattcgtgcc agcgcgccgg
     2701 gctgcccaca tggatccgct ggcggcgctt cggcatgaat aggtcccgcc ggcgtcatgc
     2761 tcgtccgtgg gtatcgtgaa gaactttcac aggtgaggtt cgcgcccgcc ggctcttcag
     2821 cgatacatcc cgtcatcacg gccttctctg acttggcagg catagtgctg atacgcgagg
     2881 taccccctgt cgaaagctca caggaggagt cccatgcgat atgtcacctc gacgatagca
     2941 gcgtgcgtgc tcgccagcgc ggccggcgtg ttcgcgcagg agcaggcgcc cccgcaacca
     3001 caaccacccg ccgccagaga gcccgcgcca gcagcgttgg aagagccagc gcctaaagcg
     3061 acgctcacag ggtgcgtcgg gcaggcgaag acgaccgacg ggggcaccgc gtacgtgctg
     3121 aacaaggctg agggtgggtc ggcgacgatg tacgtgctcg gcggaccctc gcgatcagaa
     3181 gtgtcggcga acgtgaacaa gaaggtcgaa gtgaccggac ccgtgctgga acccgcgggc
     3241 gcaaacagcg acagcgcggc ggacgcgaag gtcctgcggc cgccggtgat gctcgtcgag
     3301 gccgtgaaag tcgtggctca gagctgtacg taggcaccgt cgtcccaggt gctaggcccc
     3361 taggtccttg gtccgttctt ggtccagtgg tccgacggac cgaggactac ggacggacca
     3421 aggactgcgg acggaccaag gactacggac ggaccaagga caaagcacaa agaaccaagg
     3481 accaagctag acgccggcaa accagtgaaa gccgatgtct gagttgattc caccggtgcc
     3541 ccggccgatg ccggccatcg agtcgacggc acgaatcgac ttcaggaact tgaggttctt
     3601 gtatccgcac tgccgctcga cgcggaggcg gagcggcgcg ccgttgcccc tcggaaggtc
     3661 gcggccgttg agtccatacg ccaggatcgt ctgggggtgc gcgacctcga agagatcgta
     3721 tccctcgtac caccggtcgt aggtgtcgat gacgacgtag cgcgcggcgc tcgtgacgcc
     3781 gcccgcctcc tcgagcaccc gctg
//
LOCUS       DBNGSH010000303         2466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1212305, whole genome shotgun sequence.
ACCESSION   DBNGSH010000303 DBNGSH010000000
VERSION     DBNGSH010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1212305"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..2240
                     /locus_tag="AC16CY_07075"
     CDS             42..2240
                     /locus_tag="AC16CY_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353582.1"
                     /translation="MSRPGLVLVTLCVAATVYAARPTFWEVSTEPEFLRGEIENLAVD
                     TFGRLTLGPTSTPVYETTAPFVWTLLGAPDGSMYAGSGNDGQVYRIDTSGKGSVFFDA
                     DELEVHALALVPGGGLYVGTSPNGKIYKVEASGASAVFFDPPDRYIWSLVVDGAGNVF
                     AATGDQGKIYKIAPNGTGSEFYATKATHAMTLAFDRQGQLLAGTESPGRVFRLDQSGK
                     PFVLLESSHDEIRTLKLDARGNIYAAAVSGRPPAPLTQPQPGQAQPAPALTPSVSTEI
                     TVVAVGVAGDTQVSQPSTPTTPARGGPVGALYRISPDGAWDLLWESRSDTPYDIAIEP
                     TDTILLATGGAGKIYRLSGDPYQPTLIARANAQQVTSLLPQSEGRVVVATSNPGKIVR
                     LSAARADVGTYTSDVRDAQTVALWGTIKWVGNAPAGGRIDIVTRSGNTNPPDETWSDW
                     SAAYADRDGSQIASPRARYLQWRARFTGGRGDAPVLTSVTAAYLPRNQRPRVSSITIH
                     PPGTVFQRPFPNDPEIAGFEGGTPERPGSQAQAGSSPALGRRTYEKGLLTFVWRAEDD
                     NGDDLSYDIFYRQEGDTSWKLLKRAVTEEILVWDTTSVPNGRYTVRVVASDARSNSPS
                     TALNGSRESASFEIDNVPPVITVTASRRENGRAVIMLDVRDAHSSVQKVDYSLDGDKW
                     QAVYPKDGIADSRFEQFELVLEGEAASRGVILRATDALNNTSSVRGETGK"
     gene            complement(2248..>2466)
                     /locus_tag="AC16CY_07080"
     CDS             complement(2248..>2466)
                     /locus_tag="AC16CY_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353583.1"
                     /translation="SRLTPAQTPVGRAVAVNMVWLFVVTTVQTPDAQSAGRVAPVTAR
                     ARRVPAVDAVAPVPLNQRSARTSHSPTA"
BASE COUNT          438 a          853 c          806 g          369 t
ORIGIN      
        1 tcaaagctct aaactcaaga ctcaaaggtc gcaacctgaa cgtgtctcga cctggactcg
       61 tgctcgtcac gttgtgcgtc gccgcgacgg tgtacgccgc gaggcccacg ttctgggaag
      121 tctcgacaga gccggaattc ctgcgcggcg agatcgagaa cctggccgta gacaccttcg
      181 gtcgcctcac cctcggccct acctccacgc cggtgtacga aacgaccgcg ccgttcgtct
      241 ggacgctgct gggagcaccc gacggatcca tgtacgccgg cagcggcaac gacggccagg
      301 tgtatcgcat cgacacgtcc ggcaaaggct cggtgttctt cgacgcggac gagctcgagg
      361 tccacgcgct tgcgctggtt cccggcggcg ggctctacgt gggcacctca ccgaacggca
      421 agatctacaa ggtcgaggcg agcggcgcga gcgcggtctt cttcgatcca ccggacaggt
      481 acatctggag cctcgtcgtt gacggtgccg gcaacgtctt cgccgcgacc ggagaccagg
      541 gcaagatcta caagatcgcg ccgaacggca cggggagcga gttctacgcg accaaggcga
      601 cgcacgccat gacgctcgcc ttcgatcgcc agggccagct gctcgcgggc accgagtctc
      661 ccggccgcgt cttccggctg gatcaatccg gcaagccgtt cgtgctcctg gagtcgagcc
      721 acgacgagat tcgcacgctc aagctggacg cccgcggcaa catttacgcc gccgctgtaa
      781 gcggtcggcc gccggctccg ctcacgcagc ctcagcccgg ccaggcccag ccagcccctg
      841 cgctcacgcc cagcgtgtcg actgaaatca cggtggttgc cgtcggcgtt gccggcgata
      901 cccaggtcag ccagccctcg accccaacca ctcccgcgcg aggcggcccg gtcggggcgc
      961 tctatcgcat ttcccctgac ggagcctggg acctcttgtg ggaatctcga tccgacacgc
     1021 cgtacgacat cgcgatcgag ccgacagata cgatcctgct ggcgaccggc ggcgccggaa
     1081 agatctaccg gctctccggc gatccgtacc agccgacgct catcgcgaga gccaacgctc
     1141 aacaggtgac gagcctgctt ccgcaatccg aggggcgcgt ggtcgtcgcg acgtcgaatc
     1201 cgggaaagat tgtccggctg tcggccgcgc gcgccgacgt ggggacctac acctccgacg
     1261 tgcgcgacgc gcagaccgtc gctctctggg gcacgatcaa gtgggtgggc aatgcgcccg
     1321 cgggcggccg catcgacatc gtcacgcgct cgggcaacac gaacccgcct gacgaaacct
     1381 ggagcgactg gagcgcggct tacgccgatc gcgacggcag ccagattgcc agccctcgcg
     1441 cgcgatacct gcagtggcgc gcccggttta ccggggggcg tggcgacgcg ccggtattga
     1501 cgtccgtgac ggcagcctac cttcccagga accagaggcc gcgcgtcagc agcatcacga
     1561 ttcatcctcc gggcacggtc tttcagcgcc cgtttccgaa cgatcccgag atcgccggct
     1621 tcgaaggcgg gacgccggag agaccgggca gccaggcaca ggcgggctcg tcgccggcgc
     1681 tgggacgacg cacgtacgag aagggattgc tgacgttcgt ctggcgggct gaagacgaca
     1741 acggcgacga tctcagctac gacatcttct atcgccagga gggcgataca tcctggaagc
     1801 tgctcaagcg cgcggtcact gaagagattc tcgtctggga cacgacgtcg gtacccaacg
     1861 ggcgctacac ggtccgcgtc gtggcctccg acgcgcggtc gaattctccg tccacggcgc
     1921 tcaacgggag cagggagagt gcctcgttcg agatcgacaa cgttccgccc gtcatcaccg
     1981 tcaccgcttc ccgccgcgag aacgggcggg cggtcatcat gctcgacgtc agggacgcgc
     2041 actcatccgt gcagaaggtg gactactcgc tcgacggcga caagtggcag gccgtctacc
     2101 cgaaggacgg aatcgccgac tcgcgcttcg agcagttcga gctggtcctc gagggcgaag
     2161 ccgcttcgcg cggcgtcatc ctgcgcgcta ccgacgcgct caacaacacc tcgagcgtac
     2221 gcggggaaac agggaagtaa gcggccgtca ggccgtcggg gaatggctgg tccgcgcgga
     2281 ccgctggttg agcgggacag gcgcgacggc gtcaaccgcc ggaacgcgcc gcgcgcgcgc
     2341 cgtcaccggc gcgacacggc cggccgactg tgcatccggc gtttggacgg tcgtcaccac
     2401 gaacagccag accatgttga cggcaaccgc ccggccgacg ggcgtctggg ccggcgtcaa
     2461 tctcga
//
LOCUS       DBNGSH010000304         9370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4438721, whole genome shotgun sequence.
ACCESSION   DBNGSH010000304 DBNGSH010000000
VERSION     DBNGSH010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4438721"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1966)
                     /gene="dnaK"
                     /locus_tag="AC16CY_07085"
     CDS             complement(35..1966)
                     /gene="dnaK"
                     /locus_tag="AC16CY_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3353584.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPTVITNPEGSRLTPSVVAFTK
                     TGERLVGQVAKRQAVTNPENTIFSIKRFMGRRFTEVNEEMKMVPYTVVSAANGDVRVQ
                     SGGKDYAPPEISAMILQKLKQAAEQYLGQTVTKAVITVPAYFNDAQRQATKESGKIAG
                     LEVMRIVNEPTAAALAYGLDKKKDETIAVYDFGGGTFDISILEVGEGVVEVKATNGDT
                     HLGGDNLDQRVIEWIVAEFKKSDGIDLGKDRMALQRLKEAAEKAKMELSTVVETDISL
                     PFITADQSGPKHLQLKLTRAKFEQLVDDLLQRTVGPTRQALNDAGVDQKQIDEVVLVG
                     GSTRIPRVQQIVKDLFGKEPHRGVNPDEVVAVGAAIQAGVLAGEVKDLLLLDVTPLSL
                     GIETLGGVMTTLITRNTTIPTRKSETFSTAADSQTSVEVHVLQGERPLARDNRTLGRF
                     QLVGIPPAPRGVPQIEVTFDLDANGIVNVSAKDHGTGKEQKITITASSGLSKEEVDRM
                     MKDAESHAEEDKKRRGEIETRNRADQAVYAAEKMLQEMGDKLPSDDKAAVQTAIDGLK
                     AAVSSGDDAAITRAMELLTQVQHKAAEALYKSAGAQGASGAKGPDAQGAPGSQGSTGS
                     GGSPGGGSDVIDAEVVDDK"
     gene            2120..4483
                     /locus_tag="AC16CY_07090"
     CDS             2120..4483
                     /locus_tag="AC16CY_07090"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR3353585.1"
                     /translation="MQAHSSPIRPGTRLVVRVATRAGLLALFVLAALIGAAGGLVFVY
                     ADDVAQVSALDDYKPSTITRLTAVDGREIGEFATERRIVIGYDQINPLLRQAIIASED
                     AEFESHFGISVSRMFVTSVRDVLRGHRLRGASTITQQVARMLFLQEYMRGGVFARSGT
                     EGWERKIKEIMLAFQLEKRYTKREILAFYANHVPLDHGAYGVEAASRLYFGKSAANLE
                     LHEAASIVSIIPTPSRLSPFRNPDQNLTRRNTYVLRRMAEEGVITREEAAAAAKLPLS
                     VQGQPAPVNSLAPYFVEDIRKTLEQKYGADALYQAGLRVQTTLDVELQEAAQKAVDRG
                     LRRLDKRRNGYRRPTRNVLADGKDIAAFRTERWLQPIAPGDVVPAVVTFVPGPKDRGN
                     ARLRIASHEVELPASAFAWTRRTSPAGLFKTGDLVEVEVVDLKGDEPTKLLLEQAPAV
                     EGALIAIDNETGQVRAMVGGYSFARSKFNRATQAHRQVGSLFKPIVYTAAIDRGFTPV
                     SIFVDEPVSYEPGPNQPPYQPLNYDRKFQGPVTLRHALEDSRNIPAVKAMVEIGPAQV
                     VGYAKRFGLSGDYPPFLSVALGAVEATLVEITSAYTAFPNRGVRMEPYSVVSIADREG
                     NVLEDHRPQSHEAIRADTAFVMTNLLRGVISNGTGAAARALDWPLAGKTGTMDEYTDA
                     WFVGFDPRITVGVWVGYDEKKPLGNGETGAQAALPIWMDFMRVYIDTRADREAPPQFD
                     APGNIVFVTLDTGRPEAFINGTQPARMTSPRPSEPPPGAPAGPPAVE"
     gene            complement(4573..4791)
                     /locus_tag="AC16CY_07095"
     CDS             complement(4573..4791)
                     /locus_tag="AC16CY_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353586.1"
                     /translation="MLALLRRYWNELVSYDGGLSLLMMDALLAVPLIMAAFFYPLQVL
                     AVLLVIAVVSLAGYEGYVLWRKRHPSHV"
     gene            complement(4937..5692)
                     /locus_tag="AC16CY_07100"
     CDS             complement(4937..5692)
                     /locus_tag="AC16CY_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like protein"
                     /protein_id="HIR3353587.1"
                     /translation="MNSTHRGMAAIAVLVLWAAPSQAQTADEIIEKHLAATGGRAALG
                     KLTSRTAKGSIIIGTPVGDLAGTLEVYAKVPNKSRQFVKLDLSKLGAGEVVSDQRFDG
                     TVGYQIDTFNGNREITGGQLDAMKNGAFPTPLLRYKENGVSVSLLEREKVGDRDAYVL
                     MFAPKTGPAMRAFIDAESLMLVRTVMSVNVPQVGGDIEQVVEFSDYRDVDGVKVAFGI
                     TSANSLQTIKASLTEVTHNTEIDDASFVRPAGQ"
     gene            complement(5751..6885)
                     /gene="prfB"
                     /locus_tag="AC16CY_07105"
     CDS             complement(join(5751..6809,6811..6885))
                     /gene="prfB"
                     /locus_tag="AC16CY_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265905.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR3353588.1"
                     /translation="MALDDDLIRQHDDLNRRALELRRHLEGARLEDQLAEIEKASASP
                     TFWSNQTEAQKVMQRRRRVEDDLGLLGSLRRRTDDLSVLVEWAEAGEEVTADFSRGLE
                     ELRQEVEAAETKKMLGGEHDRANAIVTIHPGAGGTESQDWAEMLLRMYLKWAERRGFK
                     REVIDYQPAEEAGIKSATLTLTGDYAYGLMSAEAGIHRLVRISPFDQASRRHTSFASV
                     FVWPELPEDVDVEIEEKDLRIDTYRSSGAGGQHVNVTDSAVRITHMPTGIVVSCQNER
                     SQHKNRAQAMKVLKARIFDLKMKEQQAKLEQLGGEKRDIAFGSQIRSYVLQPYQMIKD
                     HRTKVEVGDVHRVLDGDLDPFIKTYLMQKASGTLGAPAAEGGE"
     gene            complement(6921..8447)
                     /gene="lnt"
                     /locus_tag="AC16CY_07110"
     CDS             complement(6921..8447)
                     /gene="lnt"
                     /locus_tag="AC16CY_07110"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR3353589.1"
                     /translation="MDYALALIAGVLLALSFPRFGHPAIAWIALVPLLLALSGWRGRA
                     GPLPGQPPLRALALGMAAGMAYFVGTVYWTSGVLSTFGGLSAPLAAFAMLLLAAYLAI
                     FPGIAAMVMARLMAGGGRPAIFFAPAAWVATEYARGFVFGGFPWVPLGNSQATVLPVA
                     QVASLAGVYGLSALVALVNASIAFALLTSGRTRLKTGGVTAALVVVVAVWGSWRVAEG
                     SLTRQGTPIRVGLIQGNVSQQDKMASTPQIRRRVFTTYIAMTRDAVRRGAQYVIWPES
                     ATPFAYGQDPAGDTALRDLAREVQVPILFGSEEIVGGERPTWYNAAFLLAPDGRTAAV
                     YRKIHLVPFGEFFPLQDWISFAAPLVKRFMPASAGESVVMLPVGSHSTSTAICYEVVF
                     PSLMREAVNKGSELLTTITNDGWYGTSSAPYQHFEMASMRAIEQGRYLARAANTGISG
                     VVDPYGRIVRASSIFEQVGLVEEARFLTSRTVYSKIGDVVAYVAIALLAVALVVVRRT
                     "
     gene            complement(8432..9085)
                     /locus_tag="AC16CY_07115"
     CDS             complement(8432..9085)
                     /locus_tag="AC16CY_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3353590.1"
                     /translation="MSDDGVREIQLNGKQLVFMFMTVTVAAVVIFLCGVMVGRGVDTA
                     RAVSIAELPAETGVDPTSGAFTSPAPATDAAASTPLSTQDDLKNLTYAQRLEAPEPPP
                     EPAAEPAIVPVPAVAGKASAPAVVVATAQKPEAPSTEPKGDGFVVQVAAVQSRSEADA
                     IAHRLSSKGFPSFVSTPGSGAPRVYRVRVGKYKDKREAVGVARRLEQEEQFKPWITR"
     gene            complement(9180..>9370)
                     /locus_tag="AC16CY_07120"
     CDS             complement(9180..>9370)
                     /locus_tag="AC16CY_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353591.1"
                     /translation="RGSLFCEVRDKVDHPLCEFYAAAFRRLMTLFQLEADVVTEQCHA
                     SGASHCAMALTMRPAGRS"
BASE COUNT         1558 a         3197 c         2984 g         1631 t
ORIGIN      
        1 acgcccaaca cgatgtacag atccattggc cgatttactt gtcgtccacg acctcggcgt
       61 cgatgacgtc ggagccgccg ccaggcgaac ccccggaacc cgttgaaccc tgcgaacccg
      121 gtgcaccttg tgcatctgga ccctttgccc ccgatgcacc ctgtgcgccc gcactcttgt
      181 agagcgcctc cgccgccttg tgctgaacct gcgtgagcag ctccatcgcc cgcgtgatcg
      241 ccgcgtcgtc acccgacgac accgcggcct tgagcccgtc gatcgccgtc tgcaccgccg
      301 ccttgtcgtc ggagggcagc ttgtcgccca tctcctgcag catcttttct gccgcgtaga
      361 ccgcctgatc cgcccggttg cgggtttcga tctcgccgcg tcgcttcttg tcctcttcgg
      421 cgtgcgactc ggcgtccttc atcatgcgat cgacctcttc cttgctgagg ccgctcgagg
      481 ccgtgatggt gatcttctgc tccttgccgg tgccgtgatc cttcgccgac acgttgacga
      541 tgccgttggc gtcgaggtcg aaggtcactt cgatttgcgg aacacctcgc ggagccggcg
      601 ggatgccaac cagctggaac cgtccgagcg tgcggttgtc gcgcgcgagc ggccgctcgc
      661 cctggagcac gtgcacctcg acgctcgtct ggctgtcggc cgcggtcgag aacgtctcgc
      721 tctttcgcgt cggaatcgtc gtattccggg tgatgagcgt cgtcatgacg ccgccaagcg
      781 tttcgatccc gagcgacagc ggcgtgacgt cgaggagcag gaggtccttc acctctcccg
      841 cgagcacgcc ggcctggatg gcggccccca ccgccacgac ctcgtcggga ttcacgcccc
      901 gatgcggctc cttgccgaac aggtccttga cgatctgctg gacgcgcgga atgcgggtcg
      961 agccgccgac gagcacgacc tcgtcgatct gcttctgatc gacacccgcg tcgttgagcg
     1021 cctgcctggt cgggccgacc gtccgctgca gcaggtcgtc aacgagctgc tcgaacttcg
     1081 cccgcgtgag cttgagctgc aggtgcttcg gaccgctctg atcggccgtg atgaacggga
     1141 ggctgatgtc ggtctcgacg acggtggaga gctccatctt ggccttctct gccgcctcct
     1201 tgagacgctg cagcgccatg cggtccttgc cgaggtcgat cccgtcgctc ttcttgaact
     1261 cggccacgat ccactcgatg acgcgctggt cgaggttgtc gccgccaagg tgcgtgtcgc
     1321 cgttggtggc cttgacctcc accacgccct cgccgacttc caggatggag atgtcgaagg
     1381 tgccgccgcc gaagtcgtag acggcgatcg tctcgtcctt cttcttgtcg aggccgtagg
     1441 ccagggcggc cgcggtcggc tcgttgacga ttcgcatcac ttcgaggccc gcgatcttgc
     1501 ccgactcttt cgtcgcctgg cgctgcgcgt cgttgaagta cgccggcacc gtgatgacgg
     1561 ccttggtgac cgtctggccg agatactgct ccgcggcctg cttgagcttc tgaaggatca
     1621 tcgcggagat ttccggcggc gcgtagtcct tgccgccgct ctgcacgcgc acgtccccgt
     1681 tggctgccga caccacggtg tacggcacca tcttcatttc ctcgttgacc tcggtgaagc
     1741 ggcgccccat gaagcgcttg atcgagaaga tcgtgttctc cgggttggtc accgcctggc
     1801 gtttcgccac ctgcccgacg agccgctcac cggtcttggt gaacgcgacg acggacggcg
     1861 tcagccggct gccctccgga ttggtaatca cggtgggctc gccgccctcc atcacggcca
     1921 caacggagtt ggtcgtgccg aggtcgatgc cgatgatttt gctcatgtct cacacgtcct
     1981 ttgcgcgtac tgccttagcc tgaagcgatt atgaaatctg agtgttgccg tgtcaatgtg
     2041 tagaggtccg cttgtcgaat caatctccga taaacgccag accgacaaca gcttctgcgt
     2101 atacttctca ggagggctga tgcaggctca ttcctcgccc atccggccgg gcacacgcct
     2161 tgtcgtccgc gtggcgacgc gagcagggtt gcttgccctc ttcgtgctgg cggcactgat
     2221 cggcgcggcc ggggggcttg tgttcgtcta cgccgacgat gtggcgcagg tcagcgcgct
     2281 ggacgactac aagccgagca cgatcacgcg cctgaccgcc gtggacggac gggagatcgg
     2341 cgaattcgcc accgagcggc gcatcgtcat cggctacgac cagatcaacc cgctcctccg
     2401 gcaggccatc atcgccagcg aagacgcgga gttcgaaagc catttcggca tcagcgtgtc
     2461 gcggatgttc gtcacgagcg tcagggacgt gctgcgtggc catcggcttc gcggcgcgag
     2521 cacgatcacc cagcaggtgg cgcggatgct cttccttcag gagtacatgc gaggcggcgt
     2581 cttcgcgcga tcgggcacag aagggtggga gcgcaagatc aaggaaatca tgctcgcctt
     2641 ccagctcgag aagcgctaca cgaagcgcga gattctcgcc ttctacgcca accacgttcc
     2701 gctcgaccac ggcgcgtacg gcgtggaggc ggcctcccgg ttgtacttcg gcaagtccgc
     2761 cgcgaacctg gagcttcacg aggccgcctc gatcgtctcg atcattccga cgccgtcgcg
     2821 cctgagccct ttccggaacc cggatcagaa cctcacgcgc aggaacacct acgtcctgcg
     2881 ccggatggcg gaggaaggcg tcatcacgcg cgaggaagcg gctgccgccg cgaagcttcc
     2941 gttgtcggtt cagggacagc cggccccggt gaactcgctc gccccgtatt tcgtggaaga
     3001 catccgcaag acgctcgagc agaagtacgg cgccgatgcg ctctaccagg ctggactgcg
     3061 cgtgcagacg acgctcgacg tcgagctcca ggaagccgcg cagaaggcgg tcgatcgcgg
     3121 cctgcgccgc ctcgacaagc ggcggaacgg gtatcgccgg cccacccgca acgtgctcgc
     3181 cgacggcaag gacattgccg cattccgcac ggaacggtgg ctccagccga ttgcccccgg
     3241 cgatgtcgtc cccgccgtcg tgacgttcgt gcccggaccc aaggaccgcg gcaacgcgcg
     3301 cctgcggatt gcgagccacg aggtcgagtt gcccgcgtcg gcgttcgcgt ggacgcggcg
     3361 cacgtcgccg gcaggactct tcaagacagg cgaccttgtc gaagtggaag tcgtcgacct
     3421 gaagggtgac gagcccacga agcttcttct cgagcaggcc cccgctgtcg aaggcgcgct
     3481 catcgcgatc gacaacgaaa ccggccaggt ccgcgcgatg gtgggcggct acagctttgc
     3541 gcgcagcaag ttcaacaggg ccacgcaggc gcacaggcag gtgggctcgc tgttcaagcc
     3601 gatcgtctac accgccgcga tcgaccgcgg attcacgccc gtgtcgatct tcgtggacga
     3661 gccggtatcg tacgagcctg gcccgaacca gccgccgtac cagccgctca actacgaccg
     3721 caagttccag gggcccgtca ccctgcgtca tgcgctggag gactcgcgca acattccggc
     3781 cgtgaaagcg atggtggaaa tcggacccgc ccaggtcgtc ggctacgcca agaggtttgg
     3841 actctccggc gactacccgc cgtttctatc cgtggcgctc ggcgcggtgg aagcgacgct
     3901 cgtggagatc acgagcgcat acacggcgtt cccgaaccgg ggcgtccgca tggagcccta
     3961 ctcggtcgtg tcgattgccg atcgcgaggg aaacgtgctc gaggaccatc ggccccaaag
     4021 ccatgaagcc atccgcgccg atacggcctt cgtgatgacg aacctgctgc gcggggtgat
     4081 cagcaacgga acgggcgcag ccgccagggc gctcgactgg ccgctggccg gcaagacggg
     4141 cacgatggac gagtacaccg acgcctggtt cgtcggcttc gatccgagga tcaccgtggg
     4201 agtgtgggtc ggctacgacg agaagaagcc gctcggcaac ggcgagacgg gggcgcaggc
     4261 ggcgctgcct atctggatgg acttcatgcg cgtgtacatc gacacgcgcg cggatcgcga
     4321 ggcgccgccg caattcgacg cgccaggaaa catcgtcttc gtcacgctcg acacggggcg
     4381 cccggaagcg ttcatcaacg gcacacagcc cgcgcgcatg acctcacccc ggccatccga
     4441 gccgccgcca ggcgcgcccg cgggcccacc ggccgtcgaa tagcgttcat ggtccggctt
     4501 cccgcctccg ccgaaggctt cggcgaggct agccgaagcg cgctctgcgc gcgaaggctg
     4561 cagccggacc tgtcacacgt gcgacgggtg tcgcttccgc cagagaacgt acccttcgta
     4621 cccggcgagg ctgaccacgg cgatcaccag caggacggca agcacctgga gcgggtagaa
     4681 gaaggcggcc atgatgaggg gaactgccag caacgcgtcc atcatcagga gagagagacc
     4741 gccgtcgtag ctgaccaact cgttccaata gcggcgcagg agcgcaagca ttacgcacct
     4801 ccttgtagcc accggccgtt cggaggtgtc aggtgagggc tggccccagg ctggcagatc
     4861 tggacgcgcc ggatcgagaa acggcgacgc aggacctcac tatagggcgg gtggggcaag
     4921 tggggcaagt gggccgctac tgccctgccg gccggacgaa ggacgcgtcg tcgatttcgg
     4981 tattgtgcgt gacctcggtg agcgaggcct tgatcgtctg aagcgagttc gcggacgtga
     5041 tgccgaaagc aaccttgact ccgtccacgt ccctgtagtc ggagaactcg accacctgtt
     5101 cgatatcgcc cccgacttgc gggacgttga cgctcatcac cgtcctgacc agcatgaggc
     5161 tttcagcgtc gatgaacgcg cgcatggctg gcccggtctt gggagcgaac atcagaacgt
     5221 acgcatccct gtcgccgacc ttttcgcgct cgagcagcga cacgctcacg ccgttctcct
     5281 tgtagcggag aagcggtgtg ggaaacgccc cgttcttcat ggcatccagc tggccgccgg
     5341 tgatttcccg gttcccgttg aacgtgtcga tctggtagcc gaccgtgccg tcgaaccgct
     5401 gatcggaaac gacctctccc gcgcccagct tgctcaggtc cagcttgacg aactggcgtg
     5461 acttgttcgg aaccttcgcg tagacctcga gggtgcccgc cagatctccg accggcgtcc
     5521 cgatgatgat cgaccctttg gccgtcctgg acgtgagctt gcccagcgcc gcgcggcccc
     5581 cggtcgctgc caggtgtttc tcgatgatct cgtcagccgt ctgtgcctgg ctcggggccg
     5641 cccaaagcac gagcacggca atcgccgcca ttcctcgatg tgtgctgttc atcacgtcca
     5701 tcctgtgttg cttcagacgt cggcggagtc acgacggttc cccactcgcc ctactcgccc
     5761 ccttcggccg ccggcgcgcc gagcgtgccg ctcgccttct gcatcaggta tgtcttgatg
     5821 aaggggtcga ggtccccgtc gagtacgcgg tggacgtcgc ccacctccac tttcgtccgg
     5881 tggtccttga tcatctggta gggctgcagc acgtagcttc gaatctggct gccgaacgcg
     5941 atgtcccgct tctctccccc cagctgttcg agcttggcct gctgctcttt catcttgaga
     6001 tcgaagatgc gcgccttgag caccttcatc gcctgcgccc ggttcttgtg ctgcgaccgc
     6061 tcgttctggc acgacacgac gatgccggtc ggcatgtgcg tgatgcgcac ggccgaatcg
     6121 gtcacgttga cgtgctgtcc gccggcgccg ctcgaccggt aggtgtcgat gcggagatcc
     6181 ttttcctcga tctcgacatc gacgtcctcc ggaagctccg gccagacgaa caccgatgcg
     6241 aacgaggtgt gccggcggga cgcctgatcg aacggcgaga tccggacgag ccgatgtatt
     6301 ccggcttcgg ccgacatcag gccgtacgcg tagtcgccgg tcagcgtgag cgtcgcagac
     6361 ttgatgccgg cctcctccgc gggctggtag tcgatgactt cgcgcttgaa tccccttcgc
     6421 tccgcccact tcagatacat gcggagcagc atctccgccc agtcctgtga ttcggttccc
     6481 cccgcgcccg gatgaatcgt gacgatggcg ttggcgcgat cgtgctcgcc cccgagcatc
     6541 ttcttggtct cggcggcctc gacctcctgc cgcagctcct ccagcccccg cgagaagtcc
     6601 gcggtcacct cttccccggc ctcggcccat tcgaccagca cggacaggtc gtcggtccgg
     6661 cggcggagcg acccgagcag tccgaggtcg tcttcgacgc ggcggcgccg ctgcatcacc
     6721 ttctgggcct ccgtctgatt gctccagaac gtggggctgg cgctcgcctt ctcgatctcg
     6781 gctaactggt cctcgagacg ggcgccctca aaggtgcctc cgcaactcga gggcacgccg
     6841 gttgaggtca tcgtgctggc ggatcaaatc gtcatcaagc gccacgggag ctcctcgttt
     6901 agtccggcta aacccggaca ctacgtacgt cgcaccacaa ccaaggcgac ggccagcagc
     6961 gcaatagcca cgtacgcgac cacgtcgcca atcttcgaat acacggtccg gctggtcaga
     7021 aagcgcgctt cttcaaccag ccccacctgc tcaaagatac tcgacgcgcg gacaatgcgt
     7081 ccgtaggggt cgacgacgcc gctgattccg gtgttcgccg cgcgcgcgag gtaccgcccc
     7141 tgctcgatcg cccgcatcga cgccatctcg aagtgctgat acggcgccga cgaggtcccg
     7201 taccagccgt cgttggtgat ggtcgtcagc agctcgctgc ccttgttcac cgcctcgcgc
     7261 atgagcgatg gaaacaccac ctcgtagcag atcgccgtgc tcgtggagtg cgagccgacc
     7321 ggcagcatca cgaccgactc gccggccgat gccggcatga accgtttgac gagcggcgcg
     7381 gcaaacgaga tccagtcctg aagcgggaag aactcgccga agggcacgag gtggatcttt
     7441 cgatagaccg ccgcggtccg gccgtccggc gcgagcagaa acgcggcgtt gtaccacgtc
     7501 ggccgctcgc ctccgacgat ttcctcgctg ccaaagagga tcggcacctg gacctccctg
     7561 gcgagatccc ggagtgccgt atcgcctgcg ggatcctggc cgtaggcaaa cggcgtcgcc
     7621 gattcgggcc agatcacgta ctgcgctccc cgtcgcacgg cgtcgcgcgt catcgcgatg
     7681 taggtggtga agacgcgacg cctgatttgc ggggtcgacg ccattttgtc ctgctgcgaa
     7741 acgttcccct ggatcaggcc gacacggatc ggcgttccct gccgcgtcag cgatccctcg
     7801 gcgacgcgcc acgatcccca gacggccacc acgaccacaa gcgcggcagt gactccaccg
     7861 gtcttgaggc gggtgcggcc cgacgtcagc agggcgaacg cgatcgaggc gttgaccagc
     7921 gccaccagcg ccgacagccc gtacactccg gccaggctcg ccacctgcgc gacaggcagc
     7981 accgtggcct ggctgttgcc aagcggcacc cacggaaagc cgccgaagac gaagccgcgc
     8041 gcgtactccg tcgccaccca tgccgccggc gcgaagaaga tcgcaggcct gccgcctcct
     8101 gccatgagcc gggccatgac catggccgca atgcccggga atatcgccag gtacgccgcc
     8161 agcagcagca tcgcgaacgc ggcaagcggg gcagacagac caccgaaggt cgagagaacg
     8221 ccgctggtcc agtagaccgt gcccacgaag taggccatcc cggcggccat gcccagcgcc
     8281 agcgcgcgca gcggcggctg cccgggcaga ggtcctgccc ggccgcgcca cccgctcaac
     8341 gccagcagca gcggaacgag cgcgatccac gcgatcgccg gatggccgaa gcggggaaac
     8401 gagagcgcga gcagtacgcc cgcgatcagg gctagcgcgt aatccagggt ttgaactgct
     8461 cttcttgttc gaggcggcgg gccacgccca cggcttcgcg cttgtccttg tacttgccca
     8521 cgcgaacgcg gtacacgcga ggcgcaccgg agccgggagt cgatacgaac gaggggaacc
     8581 ctttcgagga gaggcggtgt gcgatggcgt cggcctcgct gcgcgattgc acggcggcga
     8641 cctgcaccac gaagccgtcc ccctttggct cggtcgatgg ggcctcgggc ttctgggctg
     8701 tcgccacgac gactgcgggg gcggacgcct tgccggcgac ggccggaacc ggcacgatgg
     8761 caggttccgc ggcaggctcg ggtggaggtt caggcgcctc gagccgctgc gcgtaggtca
     8821 gattcttcag atcgtcctgc gtggacaacg gcgtgctggc agcggcgtcg gtcgcgggcg
     8881 cgggagaggt gaatgccccc gacgtcggat cgacgccggt ctcggccggc aattcggcga
     8941 tcgaaacggc ccgggcggtg tcgactcccc gcccgaccat gacgccacac aggaagatca
     9001 cgacggccgc cacggtgacc gtcatgaaca tgaagacgag ttgcttaccg ttcagctgaa
     9061 tctcgcgaac accgtcatcc gacatcatcg gcctcgtgca ttctaacacg agccggatcg
     9121 tcgagcgagg agaaacctcc cctcgccgcg aaagtcacga agacgcgggt ccggaaagat
     9181 caggaacggc ccgcgggcct catcgtcagc gccatcgcgc agtggctcgc cccgctggcg
     9241 tgacactgct cggtcacgac gtcggcctcg agctggaaca gcgtcatcag gcggcgaaac
     9301 gcggcggcgt agaactcgca cagcggatga tcgaccttgt cgcgtacctc gcagaagagc
     9361 gagccgcgca
//
LOCUS       DBNGSH010000305        23090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_533625, whole genome shotgun sequence.
ACCESSION   DBNGSH010000305 DBNGSH010000000
VERSION     DBNGSH010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 23090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23090
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_533625"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1031)
                     /locus_tag="AC16CY_07125"
     CDS             complement(30..1031)
                     /locus_tag="AC16CY_07125"
                     /inference="COORDINATES: protein motif:HMM:NF052887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8021 domain-containing protein"
                     /protein_id="HIR3353592.1"
                     /translation="MRATLGALAIIAAVVTGLVVRVGASTGPIPLNCNRACLEGVIDQ
                     YLKALVAHDPSSLPLSKDVKYTENNQVIAVGDGFWKTAQGIGSYRHVFADPEVGQVAL
                     MGTMIEAGAPLLMSLRLRIELGRITEIEAVFFKPGGGGPNNIAAMDKSGKPEDLWFRT
                     IPPAQRLSRQEMIAIADGYFTGLQKNDGKGINGTGTYPFTKDCHRIENGAPTTNVPPP
                     ANAKPGTINVFSLDCLTAFKMGLYYVVQNIHSRRYPLVDVERGVVWAHCVFDQGTVNS
                     GVLSDGTKHSYQGFNRPSSILVTEAFLIENGLIRRVEMIGPSATYHINSPWGGLSGD"
     gene            complement(1062..2297)
                     /locus_tag="AC16CY_07130"
     CDS             complement(1062..2297)
                     /locus_tag="AC16CY_07130"
                     /inference="COORDINATES: protein motif:HMM:NF046728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3353593.1"
                     /translation="MRTRTSLATLLGSLLFSLVVWAQAPATPPVPAAPAGPSAPFRPH
                     IVATGLRGGYQVVAADMNKDGKPDLIGLGSQMTELLWYENPDWTPHVITRDAPRMINL
                     AAADTDRDGIPELALAYEFNSNPTRGNPGRLAILKVSGDPRGVWSLKDIDVLPTSHRV
                     RFATIGGRPILVNAPILAASVKDGFADSAHTPNPLRAYQPPEWKPETITEANLGVVHG
                     LFTGDWDGNGSDDVLTAGYVGVHAHSLGTNGAWTRTELAKGHPADWPGGGASDIAVGR
                     MNGRRFFVTNEPFHGNQVVVYQQAADGTWPRNVIETEIVNSHSLVLVDSDGDGSHEIV
                     SGGTRGAAGTPRGTKPGVFFYKAADASAQRWERMVLDPEIAANACVAVDINGDKKMDV
                     ACIDNGNPWTLRWYENSRP"
     gene            2429..2995
                     /locus_tag="AC16CY_07135"
     CDS             2429..2995
                     /locus_tag="AC16CY_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3353594.1"
                     /translation="MPATRSDILRELVETLRKVGAQSVLLSDTVATLVGLNSTDLECL
                     DLLDIAGPTTAGRLAQHVGLTTGAMTAVIDRLEHAGLVRRLRDPQDRRCVRVEAVRRR
                     FRHVTALYQRLIDATERLHQDYDDEELALVVDYLTKALALAADHMAWLQTQRPLRPQA
                     RPRRRRKRPARGVVPPSAGNGGSLGGQP"
     gene            3049..4593
                     /locus_tag="AC16CY_07140"
     CDS             3049..4593
                     /locus_tag="AC16CY_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3353595.1"
                     /translation="MFDRRNVRTVAACVLAMIPAAAAAQAPEPAAVPAGPLSLEQVLS
                     LAEPRSEAVSIAVAGISRADGDQVRARSGQRPQLTGSASYDRALASEFEGVFDDFQFP
                     GGGGGTESGFEDLPFGRANTWRATLSFSQSLYSGGRNGAQMTLAAVGREAAGLGLTTA
                     RAQLQLDVTQAYFNAVLAQQLVTIATATLEQANATLRQVEASFKAGTQPEFEVLRARV
                     SRDGQLPILIRRRVDREIAYLRLKQLLELPADYDLQLADALGAENLPPPTAFAERVTA
                     AERSVLDQDPVAVVQGKGILQPRIAVTQADTAVRQREASLRLTEAQAKPSVALLSTYS
                     RIGYPDNAVAPTFDRSTWTVGATVSVPILTGGRQRGDEMVAKAELEQSQKQRQQIDEL
                     AALETRGAWAELFAARAAWEATAGTVQQANRAYEIAEVRYKAGVSTQLELSDARVQLQ
                     QAEANRALTARDLQVARARVALLPELPLSGAAGAAATAPQAQPQAAPAPTQPSGSGQF
                     RNAFAQ"
     gene            4635..5717
                     /locus_tag="AC16CY_07145"
     CDS             4635..5717
                     /locus_tag="AC16CY_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3353596.1"
                     /translation="MRGVIGVLLGAAMTVSCATEAEKAPGDAVPAVQIGAENIVTVSA
                     GTVVVGPIVSGELTASRESTVRAELGGAVVEVNVDEAQKVERGELMARIETRTLEDAK
                     LSSVSALRSAENQLAVARREAERAASLVEAGAVAARDLDVARNAVTSAEAQVADARSR
                     LASAERALSDTEIRALFAGIVSRRAVHAGDVVSPGTELFRIIDPSSMRLEASVTAEDL
                     PVLRVGATVQFRVRGYERSFQGRIERIAPQADPSTRQVPIYVSIPNPGGELVAGLFAE
                     GRVVSSTAKGVVAPIQAVNTTGPEPWVLRVVGGTTERVSVTLGLRDPRSEQVLIASGL
                     KDGDTLLSGAAQGITPGTPVRVITTK"
     gene            5722..8835
                     /locus_tag="AC16CY_07150"
     CDS             5722..8835
                     /locus_tag="AC16CY_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3353597.1"
                     /translation="MFISDTAIERPVLTIVAMLMFVVFGVVALLQLDTDEFPEIDAPV
                     VVVSIPYPGASPDVVEREVTDPIEEVLSGISGIDRMTSNSLDSFVNIIVEFDFSKDPR
                     VASQEVRDKISEIRNELPTEMEEPILTQYDPADRPIMSLTLSSPGLSGAELTRMADPE
                     ITRHLRSISGVASVDLVGAIERELVVEIRPRDLQAAGVSVGQLVLALQSQNLASPVGR
                     LEGQLDERTIRLKGRLDSPADFKQLVVSQAQGRVVRLGDLADVRDGTEEPRSFALYDD
                     EVAVGIDIIKSTGFSTTAVAEQVRSRVDEIQKTLPAGVTLRVVTDSGVRVASSVAGVQ
                     EALLEGAALTVVVVFLFLNSWRSTVITGLALPVSVLASFVAVWAYGFTLNTMSLLGLS
                     LSIGILIDDAIVVRENIVRHVQMGKGHYQAAIDGTNQIGLAVTATTLSIVVVFIPIAF
                     LGGIAGQWFKPFGLTIVCSVLVSLFVSFSLDPMLSAYWPDPKTPLEQRPLLSRLLGRF
                     NHWFDRRAEAYKGVIAWALDHRFAMVFLALATFVAALALPAYGVIGAGLIPEQDQSQF
                     NVDLETPPGSNLAYTRLKAQEVSRMARRRPEVDYVYTAIGGQGDAVDEGRVYVKLVSK
                     GERERSQAEVVADLRAELATVAGLTTSISTGWEPGQKQIQLQLRGSDASELTRLAQAV
                     AAEMRQVPGAVEVGLSTKGQKPELDVQLDRGLAGSIGVTVGQVAQALRPAFAGIDVGD
                     WIDPSGETRDVTIRMAPESRTLVADLESLPLVVAGAQNAVIPLGQVARVSPSIGPARI
                     DHLNRDRVITVEANTQGRPLSAVVGDTMARVDQAVTFPAGYAISQGGETEEQQEIFGQ
                     MFVAIGVAIMLMYFVLVLQFGSFLEPLAILLSLPLSLIGVMLALLITGKPLEIMSMIG
                     VILLVGIVAKNAILLIDFAKWSEEEGLDRREALIQAGRVRLRPILMTTFALVAGMVPV
                     ALGGGEGGDFRAPLGITVIGGVVTSTLLTLLVIPTVYEILADTRDWLAARLFRSKGKA
                     VPAAVES"
     gene            8841..9659
                     /gene="hemQ"
                     /locus_tag="AC16CY_07155"
     CDS             8841..9659
                     /gene="hemQ"
                     /locus_tag="AC16CY_07155"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HIR3353598.1"
                     /translation="MHNPFVPESLEGWSILHLMYRVRWDRLKQASQADVQRMADEAGR
                     ALSVPDAGATAVAQMLGHKADLMFIAFRRGFEQLAQAQLSLSRAELCAYLEPTTSYVS
                     IVELGMYEMTAKIHEQLGALHKPGSEAFDRAFDAEMETQRQRVMNRLFIEVPKSRYVC
                     FYPMNKRRGETVNWYSESFEKRAAMMREHGMIGRHYTGKVTQVISGSIGYDDWEWGVD
                     LFAQDPLVFKKLIYEMRFDEASAKYAEFGPFYTGLQFDLTKLSAYLSGETPSLD"
     gene            9875..10348
                     /locus_tag="AC16CY_07160"
     CDS             9875..10348
                     /locus_tag="AC16CY_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3353599.1"
                     /translation="MNPQEMFVTRLRRHRQRARVSIEAIAENLRIRPELIEAFENNDL
                     SAWPRGLYARAWIRTYALSVDLDPVDTLEEFCRLFPQGDRRTGGTIQEIAAIVASPSE
                     YRDEFNHPVGRRSSDERRAEAPRTAWHAFFTQPGRALWSRLTGSQPAFRDRSISS"
     gene            10434..11018
                     /locus_tag="AC16CY_07165"
     CDS             10434..11018
                     /locus_tag="AC16CY_07165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017323504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3353600.1"
                     /translation="MRHQKLLWAAILVVSTAWACKSPQPQPAAEPAAAAPDRGPDVIF
                     VPTPQEVVDAMLKLAKVTKNDVIYDLGSGDGRIPITAAKTYGARATGIDIDPQRIKEA
                     TENLKAAGVGDRVRFLNQDLFTTNISEATVVTLYLLPSLNVKLLPKLNKELKPGTRIV
                     SHAFDMSADGVERKPLETVTVNGRSIYFWTIPIK"
     gene            11133..11921
                     /locus_tag="AC16CY_07170"
     CDS             11133..11921
                     /locus_tag="AC16CY_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HIR3353601.1"
                     /translation="MEQAFVITLREGLEAFLIVAISLAYLRKTGRPQLIPAVHWGIGV
                     SILVSIGAAILFQQASNQALWEGVLALTAAVLVGTLIVHMWRHAKRMKGEIESRLEST
                     ALKSGTKALVGVFLFTLLMITREGMETALLMGTLVFQEAASSILTGAIGGTLCAAFVA
                     WLWSRYGHRVNLALFFQVTAIFLGIFVVQLAIYGLHELTEANLFQGSEALHWATEPYG
                     PDGRYGQFLTYLMVALPVAWLCFAALFGHRPAHRSPRMEQPAAR"
     gene            11940..12992
                     /locus_tag="AC16CY_07175"
     CDS             11940..12992
                     /locus_tag="AC16CY_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HIR3353602.1"
                     /translation="MGRRQYLDWLRGVAVLIMVETHLVDAWTRAPDRSEAAYRWAMFV
                     GGFSAPLFLFLAGIALALAAGARTRKGAHLSEAAALARRRGWQILGLAFLFRLQSFAI
                     SGGVFPQTLLKVDILNVMGLGMLLAALLWARGRRLSSRAAWLAAVAVLVVLATPIVRA
                     MALPSWLPYPLAWYLQPVPDSGAFTLFPWLAFLLIGSAVGLWLDAAGTGTEERRVVVA
                     LSLAGPAVALGAYAATFLPSMYAGTPSWSASPSIFFLHLGVVMTAIPAAYAWRRRASG
                     WSPLREFGVASLFVYWIHVEMVYGIVSLPLHRALGFEQAMAAYAALCVFLYALVKLKD
                     RGLRTLAPSHPRTLAP"
     gene            complement(12989..15151)
                     /locus_tag="AC16CY_07180"
     CDS             complement(12989..15151)
                     /locus_tag="AC16CY_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR3353603.1"
                     /translation="MARAVKVTDGPVLDGRVSDDPVWMNLEPLVEFWQTAPDAGEPSS
                     ERTEVRIVYTDDTLYVGVVLTDRDPSGLVVSDARRDSPLDDSDSFRMVLDTYRDRQNG
                     FVFATNPAALEYDGQVTGEGNNNPFGGGGGGGGGGGGRQSSGSGGGFNLNWDGVWDVR
                     TTTSESGWSAEFAIPFRTLRYEPGTGRQWGVNFQRTIRRRKETAYWSRLPIQFDLMRV
                     SMAGVLTGLDLPVPQNLQLVPYALGEARQGGLSNPGAKYFGDVGLDAKYSVTPSLTLD
                     ATVNTDFAQVEVDEQQINLDRFNLFFPEKRPFFLENAGLFAVGSPSEAEVFFSRRIGI
                     GELGGEIPIIGGARLSGRAGPVNVGLLNMQTGTVFGQAANNFTVARVRRDFEGRSSVG
                     AIFVGRLATGNVADDDDQNLAYAADGRWGIGRTGLVSGFIARTATPGFPDSQHAFQVL
                     ARNDTQPLSLSGGYTETGRNFNPEVGFLSREGGFRKVEGQVFSRLRPGEASKFQEIRP
                     HSNYRAYWNHDGILETGFWHLDSAWELRSAWEFSTGVNLTHEGVVEPFSIYPGVVVPV
                     GVYDHTEAQLSLTSNEGAPVSGRLQLNAGGFFGGHRVRLTPQFRVRVGENFNTEVAWD
                     RNDVNLPYGQFVTNLGRTRVSYSFSPRVFVQGLVQYNDRDNIWSTNLRFGWLQQANAG
                     VFIVYTDSHFIDDELGLGPRVPNRSLIVKVSRIFDVLN"
     gene            15419..16549
                     /locus_tag="AC16CY_07185"
     CDS             15419..16549
                     /locus_tag="AC16CY_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HIR3353604.1"
                     /translation="MINVAAIRMQQFGVRFYQASLTAGDIDKLVRFEVLNYGESGQPV
                     RGGAKKAPSKVNWDLLERRIASSERAYQRQIIRRKIDELVQYYEQCRQARDLPSIAGA
                     VIISCDEKLTFEPVEDGASIGRLKVPEREGILRAIDGQHRLLALHADIDNIREDDFTV
                     PAIIFDRLPEDHVVQMFVTINAKHTRLNASHLVSLSGRQLYRDDALAAAHDVVRGLND
                     REDSPLQGEIKLLGVGHGRVAQAPLAQELKKLFASDAFGGPRKSADFREDAKRFFVNY
                     FKQVSVVFSVAWGGRKYSIKSATALRAFIRVAPDVVRRLDQEHADRSDFRAIGRVIAP
                     WGRRIGNLRFESEGVWKRAGTTVDSLARELKLALEYPEGAGV"
     gene            16765..17541
                     /locus_tag="AC16CY_07190"
     CDS             16765..17541
                     /locus_tag="AC16CY_07190"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HIR3353605.1"
                     /translation="MRIDLNADVGESFGAYKLGHDELLMEAVTSANVACGFHAGDPGV
                     MRDTVAMAREQGVAVGAHPGFPDLAGFGRREIHATPREIEDLVVYQIGALAAVAAAQG
                     VRLQHVKAHGALYNMAAREPALADAIARATASVDRSLVLFGLAGSRLIEAGQRAGLRT
                     AREAFADRAYRGDGALVPRSQPGAIVETPNEVVSRVVSMVTRQAITAIDGSQVRVEAD
                     TICVHGDTPGAANLAKRIRHSLVEAGVDVVSVGSTAGGVR"
     gene            17738..18289
                     /locus_tag="AC16CY_07195"
     CDS             17738..18289
                     /locus_tag="AC16CY_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3353606.1"
                     /translation="MDVSDAALIQRSRSGDLTAFDALMTRYQGLVFTVAYACARDRDD
                     ALDISQNAFMKAFEHLDSLRDEASFRSWIARIARREGIDWARRNRHRRQFVDPEEVHL
                     PDAAPSQEARLLSDECGRRLAARLDRLNRRYRVTVSLRYVEGMSIAEIAAVLRCSEAM
                     VKNMLFRSLRRLRQELTEARQVP"
     gene            18286..19200
                     /locus_tag="AC16CY_07200"
     CDS             18286..19200
                     /locus_tag="AC16CY_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353607.1"
                     /translation="MTVDRCQRWSEQFLAGEMTALEHEAFLRHARSCEACNSLVLLDA
                     ELRVAPPVSRLTAAEGDGVRRSVLRQLHALQERRQAAGQRPRGVGTALLAAAAVLLVG
                     VGMVVGRFWAGAARGEDGEALTGLIKQAALGSLTLADVENSPYVFTNVAFRSVDAEQV
                     AVSFDVSRHLELTAHRADPLIRQLVVQAVVNPSPIGSRLKAISYAEGVADRAVMDALL
                     IAMLDDPSAEVRLRALAALAPHKADPEIQSALLTVLTTSDSVPMRLQAIDYLVKGRVD
                     PQRLRSAVSMLDPEEHAALLVRASGYLQ"
     gene            19212..20354
                     /locus_tag="AC16CY_07205"
     CDS             19212..20354
                     /locus_tag="AC16CY_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3353608.1"
                     /translation="MTDTIARTAGVILAAGLSLSAQGKTFTDEIRVSPGQALELRLDT
                     RADVTITGSDDAWVRVQAIPGDQGASDGVVTTRPTSRGVQIVSKGAGETRFLTGNPAR
                     LTIVVPRRFDVHITSMVGNLRVENVEGQIDGETKGGNLTLTGLSGGLNLTTRGGDIHV
                     SNSRLDGKVVTMGGSVTLEDVAGHVNSVSMGGSVVSRTARRNGGAAPGPPGELRITTT
                     GGAIDVAEAMAGAIVTTTGGAIHIQQAAGYVRATTTGGEIRLDSVDGWIEATTPGGGV
                     IATMVGDPDRGDRHVRLVSVGGDITLTVPESLGMRFDITLAYTRNSRQNYEIHSDFPL
                     QIRRTDWEYATGDRRRHIYGTGTAGNARHLVHIETVNGDVTVRKAP"
     gene            20392..21336
                     /locus_tag="AC16CY_07210"
     CDS             20392..21336
                     /locus_tag="AC16CY_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3353609.1"
                     /translation="MALAVPFQSPQPASWKDPSSHVRRFIDVAQNVRLEVLDWGGAGR
                     PLVLLAAGGDTAHVFDDFAPKLTGDFHVYGITRRGFGESTFAPVTSGADTFGDDVLAV
                     LDALKLEKPVLAGSSIAGQELSSIGTRRPDRVAALVYLDAAYQYAFDNGKIPTFEDIQ
                     AVGFPQRPPPTDDDLATFDGLRQYYTRLLGFTYPEAELRQKRLVTEQGGVGAERVFPG
                     GRSLLITMTKFVEIRTPALFLVSGQNPGRWANSSTDPKVRQQVAGLQAVLARQAKQIE
                     DSIPSARVVQLPDANHYVFLSNEADVLREMRAFLGRLR"
     gene            complement(21425..>23090)
                     /locus_tag="AC16CY_07215"
     CDS             complement(21425..>23090)
                     /locus_tag="AC16CY_07215"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR3353610.1"
                     /translation="VPQSLAPYPERTLLDYLEELARDNADKPALFFKGTTVTYGRLDK
                     ESTAFAAALSGLGVRKGDRVALILPNCPQFLIAQYGAWKAGAIVVPVNPTYTEREIEQ
                     SLTSSDVHTVVTLTAFYDRLNRARPRTSVRHVIATSIKDYLPPVLRVLFTLLKEKKDG
                     HRISIAPGDLWMSDLLRSHSASPRPTAKVGPNDRAVILASGGTTGTPKGVVGLHRHYV
                     AAGRQLYEWTKSAKRPWDAIMLPLPLFHVYANVGVQPLALLGPNPLSLVPNPRDIDDL
                     LKTIKQVRPVVFNGVPTLYTAMLNHPEVRAGKVDLSSIRLCFSGSAALMAETKRQFEE
                     KTGARIIEGYSLTEGMMACLVNPVAGPNKIGSIGIPVPDVEVRIVEAETGTADMPTGE
                     VGELIMCAEQYMSEYWDNPDETSQTLRTREDGRVWLHTGDLAYMDRDGYVFLVDRKKD
                     MLKTSGFQVWPREIEEVISAHPAVLEVGVAGIPDELKGEVAKAWVVLKHGQSASEDEI
                     RAFCRERLAPYKVPAKVDFRKELPKTMVGKVLRRVLAAEGDAARHY"
BASE COUNT         3897 a         7444 c         7754 g         3995 t
ORIGIN      
        1 ggggcctgag gcttgggccc tgattcctgc tagtccccgc tcagtccccc ccacggcgag
       61 ttgatgtggt acgtcgccga gggcccgatc atctcgacgc ggcgaatcaa gccgttctcg
      121 atcaggaacg cctcggtgac caggatgctg ctcggccgat tgaagccctg gtagctgtgc
      181 ttcgtgccgt cggagagcac acccgagttc accgttccct ggtcgaagac gcaatgcgcc
      241 cacacgacac cgcgctcgac gtccacgagc ggatagcgcc ggctgtggat gttctgcacg
      301 acgtagtaaa ggcccatctt gaacgcggtc aggcagtcga gcgagaagac gttgatcgtt
      361 cccggtttcg cgttcgccgg cggcggtacg ttcgtcgtcg gggcgccgtt ctcgatccgg
      421 tggcagtcct tcgtgaaggg atatgtgccg gtgccgttga tgcccttgcc gtcgttcttc
      481 tggaggcccg tgaagtaccc gtcggcaatc gcgatcatct cctggcggga caggcgctgc
      541 gcgggcggga tcgtacggaa ccagaggtcc tcgggcttgc cggacttgtc catcgccgcg
      601 atgttgttcg gaccgccgcc accgggcttg aagaagaccg cctcgatctc ggtgatgcgc
      661 ccaagctcga tgcggagacg caggctcatg aggagcgggg cgcccgcttc gatcatcgtg
      721 cccatcagcg cgacctggcc gacctcagga tcggcgaaga cgtggcgata gctgccgatg
      781 ccctgcgccg tcttccagaa gccgtcgccc accgcgatga cctgattgtt ctcggtgtac
      841 ttcacgtcct tcgagagcgg gagactcgac ggatcgtgtg ccacgagcgc cttcaggtac
      901 tggtcgatca cgccttccag acacgcgcga ttgcagttga gcggaatcgg tcccgtcgac
      961 gcgcccacgc gcacgacgag acctgtcacg acggccgcga tgatggctaa ggcaccgagc
     1021 gttgcacgca tgaaggtcct ccaaatcaac aatgatgtcg ttcacggccg gctgttttca
     1081 taccaccgca acgtccacgg gttcccgttg tcgatgcagg cgacgtccat cttcttgtca
     1141 ccgttgatgt cgacggccac gcaggcgttg gcggcgatct cgggatcgag caccatccgt
     1201 tcccaccgct gcgcgctcgc gtcggccgcc ttgtagaaga acacgcccgg tttcgtgcct
     1261 ctcggcgtgc cggccgcgcc gcgcgtcccc cccgacacga tttcgtggct gccgtccccg
     1321 tcgctgtcga cgagcacgag cgaatgcgag ttcacgatct cggtctcgat cacgttgcgc
     1381 ggccacgttc cgtcggcggc ctgctggtag acgacgacct ggttgccgtg gaacggctcg
     1441 ttggtgacga agaacctgcg gccgttcatc cgtcccaccg cgatgtcgct ggcgccgccg
     1501 cccggccagt ccgcggggtg gcccttggcc agctcggtcc tcgtccacgc gccgttggtt
     1561 ccgaggctgt gcgcgtgcac gcccacgtag ccggcggtca gcacatcgtc gctgccgttg
     1621 ccgtcccagt cgccggtgaa cagaccgtgg acgactccaa ggttggcttc ggtgatcgtc
     1681 tcgggtttcc actcgggcgg ctggtaggcg cggaggggat ttggagtatg cgccgaatcg
     1741 gcgaacccgt cctttacgct ggccgccagg atcggcgcgt tgacgaggat cggccgcccg
     1801 ccgatggtag cgaaccgcac acgatgcgac gtcgggagca cgtcgatgtc cttcaggctc
     1861 cagacacctc gcggatcgcc gctcaccttc agaatcgcga gcctgccagg gttcccgcgg
     1921 gtggggttgg agttgaactc ataggcgagc gcgagctcgg ggatgccgtc gcggtccgtg
     1981 tctgccgccg ccaggttgat catccgcggc gcgtctctcg tgatcacgtg aggcgtccag
     2041 tcggggttct cgtaccagag cagctcggtc atctgcgagc cgagcccgat gagatccggc
     2101 ttgccgtcct tgttcatgtc ggcggccacc acctgatagc cgccgcggag gccggtcgcg
     2161 acgatgtgcg gacggaaggg cgcggatggt ccggccggcg cagccggaac cggcggcgtc
     2221 gcgggcgcct gcgcccacac gaccaggctg aacagaaggg agccgaggag cgtggccagc
     2281 gaggttcgtg tacgcatgtg cgcgattcta atcgcaccgc gcgtcgacag agtggcttcc
     2341 ggccatttag ggacttgccg ctatacgaac actgcctctt gtgtcaagta tcttgacgtt
     2401 tgagatactt atcaactgag cgaactagat gcccgccacc cgatccgaca tcctcagaga
     2461 gctcgtcgaa accctgcgca aggtcggcgc ccagtcggtg ctcctcagcg acaccgtggc
     2521 gacgctggtc gggctgaact ccaccgacct ggaatgcctc gacctgctcg acatcgcggg
     2581 accaacgacc gcgggccggc tggcccagca cgtgggcctg acgaccgggg cgatgacggc
     2641 tgtcatcgac cgtctcgagc acgccggtct cgtgcgccgg ctccgggatc cgcaggaccg
     2701 gcggtgcgtg cgggtggagg cggttcggcg caggttcagg cacgtcacgg cgctttacca
     2761 gcgtctcatc gacgccaccg aacggctgca tcaggactac gacgacgagg agctcgccct
     2821 cgtcgtggac tacctgacca aggcgctggc gctggcggcc gaccacatgg cctggctcca
     2881 gacccagcgg ccgctgcgcc cgcaggcgag gccccggcgt cgtcggaaga ggcccgcccg
     2941 aggagttgtc ccgccgagcg cagggaacgg cgggtcgctg ggggggcaac cgtgacccgc
     3001 gttcaggccg cccgcacgaa cgattcacta tcgaggatgt tcacgaccat gtttgatcga
     3061 cgcaacgttc gcaccgtcgc cgcctgtgtg ctggcgatga ttcccgccgc cgccgccgcg
     3121 caggcgccgg aaccggccgc ggtgccggct ggcccgctct cgctcgaaca ggtgctgtcg
     3181 ctggccgagc cgagaagcga ggcggtgtcg atcgcggtgg ccggaatcag ccgcgccgat
     3241 ggcgaccagg tccgggctcg aagcggtcag cgaccgcagc tcaccggctc agcgagctac
     3301 gaccgtgccc ttgcctccga gttcgaaggc gtgttcgacg actttcagtt tcccggaggg
     3361 ggcggcggca ccgagagcgg gttcgaggac ctgcctttcg gccgcgcaaa cacctggcgg
     3421 gcaacgctgt cgttctccca gagcctgtac tcgggcgggc gtaatggggc ccagatgacg
     3481 ctcgccgccg tcggccgaga ggccgcgggg ctggggctca cgacggcgag ggcgcaactg
     3541 cagctcgacg tgacgcaggc ctacttcaac gcggtgctcg cgcagcagct cgtcacgatc
     3601 gccaccgcga cgctggagca ggcaaacgcg acactgcggc aggtcgaagc ctcgttcaag
     3661 gccgggacgc agccggagtt cgaggtactg cgcgccaggg tctcgcgcga cggtcagctg
     3721 ccgatcctga tccggcggcg cgtggaccgc gagatcgcgt acctgcggtt gaagcagttg
     3781 ctcgagctgc ccgccgacta cgacctgcag ctcgccgacg cgctgggcgc tgaaaacctt
     3841 ccgcccccga cggcgttcgc ggagcgtgtc accgccgcgg aaaggagcgt gctcgaccag
     3901 gatccggtcg ccgtggtcca gggcaagggc atcctgcagc cccgcatcgc ggtgacgcag
     3961 gccgacaccg ccgtgaggca gcgcgaggcg tcgctgcggc tcaccgaggc gcaggcaaag
     4021 ccgagcgtgg cgctgctgtc cacctacagc cgcatcgggt atcccgacaa cgctgttgcg
     4081 cccaccttcg accgctcgac gtggacggtc ggcgcgacgg tgagcgttcc gatcctgacg
     4141 ggcggacggc agcgcggcga cgaaatggtc gcgaaggccg agctcgagca gtcgcagaag
     4201 cagcggcagc agatcgacga gctcgccgcg ctcgaaacgc ggggagcgtg ggccgagctg
     4261 ttcgccgccc gcgcggcctg ggaagcgacc gcggggacgg tacagcaggc gaaccgcgcg
     4321 tacgaaatcg ccgaggtccg ctacaaggcg ggcgtgtcca cacagctcga gctgtccgac
     4381 gcccgggtgc agctgcagca ggccgaagcg aacagggctt tgacggctcg cgatctgcaa
     4441 gtcgcgcggg cgcgggtggc gctgctgccg gagctgccgc tgtcgggcgc ggccggcgcc
     4501 gcagcgacgg ctccacaggc gcagccgcag gccgcgccag cgccgaccca accttcggga
     4561 agcgggcagt tcagaaatgc atttgcgcag tgaagtgcgg gtgacaattg gaaaaccgaa
     4621 tgatgacgcg aggtgtgaga ggtgtgattg gggtcctgct gggcgcggcg atgaccgtct
     4681 catgcgcgac cgaagcagag aaggcgcccg gggacgcggt tccggccgtg cagatcgggg
     4741 cggagaacat cgtcacggta tcggctggta cggtggtggt gggaccgatc gtgtccggcg
     4801 agctgacggc ctcccgcgag tcgaccgtgc gcgcggaact cggcggcgcg gtcgtggaag
     4861 tgaacgtgga cgaggcgcag aaggtcgagc gcggcgagct gatggcccgg atcgagacgc
     4921 gcacgctcga ggacgcgaag ctgtcgagcg tgtcggccct ccgttcggcg gagaaccagc
     4981 tggcggttgc gcggcgcgaa gccgagcggg ccgcgagcct ggtcgaggct ggcgccgtcg
     5041 ccgcgcgcga cctggacgtc gcccgcaacg ccgtgacgtc ggcggaagcg caggtcgcgg
     5101 acgcgcgatc gaggctggcg agcgccgaac gcgcgctgag cgatacggaa atccgcgcct
     5161 tgttcgcggg gattgtctcc aggcgcgccg tccacgccgg cgacgtggtg tcgccgggaa
     5221 cggagctgtt ccgaatcatc gatccctcgt ccatgcgtct cgaggcatcg gtcacggccg
     5281 aggacctgcc ggtgcttcgc gtgggcgcaa cggtgcagtt ccgggtgcgc ggctacgagc
     5341 gatccttcca ggggcgtatc gaacggatcg cgccccaggc ggatccgtcc acgcgacaag
     5401 tgccgatcta cgtgtcgatt cccaacccgg gaggcgagct cgtggccgga ctcttcgccg
     5461 aaggtcgcgt ggtcagttcc acggcgaaag gagtggtcgc gccgatccag gccgtgaaca
     5521 ccaccgggcc tgagccgtgg gttctccgcg tcgtgggcgg gacgaccgag cgcgtcagcg
     5581 tcacacttgg actgagggat ccccggagcg agcaggtcct catcgcctcg ggcctgaagg
     5641 acggagacac cctcctcagc ggcgcggcgc agggaatcac cccgggaacg ccggtaagag
     5701 tcatcacaac gaagtaacgc catgttcatt tcagacaccg ccatcgaacg acctgtcctg
     5761 accatcgtcg cgatgctgat gttcgtcgtg ttcggcgtgg tggcgctcct ccagctcgac
     5821 accgacgagt tcccggagat cgacgccccc gtggtcgtcg tctcgattcc gtatcccggc
     5881 gcatcacccg acgtcgtcga acgcgaggtg accgatccga tcgaggaagt cctgtccggg
     5941 atcagcggca tcgaccggat gacgtcgaac tcactcgaca gcttcgtcaa catcatcgtg
     6001 gagttcgact tctcgaagga cccgcgggtg gcgagccagg aggtgcgcga caagatctcg
     6061 gagattcgca acgagctgcc gacggagatg gaagagccca tcctgacgca gtacgaccct
     6121 gccgatcgtc cgatcatgtc gctgacgctg tcctcgccgg ggctgagcgg agccgagctg
     6181 acgcgaatgg ccgaccccga gatcacgcgg cacctgcgct cgatctcggg cgtggcatcg
     6241 gtggatctgg tcggagcgat cgagcgggag ctggtcgtcg aaatccgccc gcgcgacctg
     6301 caggcggccg gcgtcagcgt ggggcagctg gtgctggcgc tgcagtcgca gaacctcgcg
     6361 tcccccgtgg gacgcctgga aggccagctc gacgagcgga ccatacggct gaagggccgg
     6421 ctcgactctc cggccgactt caagcaactc gtggtgtcgc aggcgcaggg aagagtcgtt
     6481 cgtctgggcg accttgcgga cgttcgcgac ggaacggaag agccgaggtc cttcgcgctc
     6541 tacgacgacg aagtcgccgt ggggatcgac atcatcaagt ccaccggctt cagcaccact
     6601 gccgtggccg agcaggtccg gtcccgcgtc gacgagattc agaagaccct gccggcgggc
     6661 gtgacccttc gcgtcgtcac cgattccgga gtgcgtgtgg cgagctcggt ggctggcgtc
     6721 caagaggcgc tgctcgaggg cgcggccctg accgtcgtcg tggtcttcct gttcctcaac
     6781 tcctggcgat cgacggtcat caccggcctg gcgctgccgg tgtccgttct tgcctcgttc
     6841 gtggccgtat gggcctacgg cttcacgctg aacacgatgt cgctgctcgg cctatcgctg
     6901 tcgatcggca tcctgatcga cgatgcgatt gtggtgcggg agaacatcgt gcgccacgtc
     6961 cagatgggca agggccacta ccaggccgcc atcgacggca ccaaccagat cgggctcgcc
     7021 gtcacggcga cgacgttgtc gatcgtggtg gtgttcatcc cgatcgcgtt cctcgggggg
     7081 attgccggac agtggttcaa gccgttcgga ctgacgattg tctgttcggt cctcgtctcc
     7141 ctgttcgtgt ccttctcgct cgatcccatg ctctcggcgt actggcctga cccgaagacg
     7201 ccgctcgagc agcggccgct gctgtcgcga ctgctcggac ggttcaatca ttggttcgac
     7261 cgccgtgccg aggcgtacaa gggcgtgatc gcgtgggcgc tcgaccacag gttcgccatg
     7321 gtcttcctgg cgttggcgac cttcgtcgcg gccctggctc ttccggcgta cggcgtgatc
     7381 ggcgccggac tgatcccgga acaggaccaa tcgcagttca acgtggatct ggagacgcca
     7441 cccggatcga acctcgccta cacccgtctg aaggcgcagg aagtctcccg catggcccga
     7501 aggaggccgg aggtcgacta cgtctacacg gccatcggcg gacaggggga cgcggtcgac
     7561 gagggccggg tctacgtcaa gctcgtgtcg aagggcgagc gggagcgcag tcaggccgag
     7621 gtcgtggccg atttgcgggc ggagctcgcc acggtggcag gacttacgac gtccatcagc
     7681 accgggtggg aaccgggcca gaagcagatc cagctgcagc tgcggggatc cgacgcgtcg
     7741 gagctgacgc gcctcgcgca ggccgtggcc gccgaaatgc ggcaggtgcc tggcgccgtg
     7801 gaggtcggcc tgtccacgaa gggtcagaag cccgagctgg acgtgcagct cgatcgtggc
     7861 ctggcaggct cgatcggggt gacggtgggt caggtggcac aggcgcttcg ccccgcgttt
     7921 gcggggatcg acgtcggcga ctggatcgac ccgtctggcg aaacgaggga cgtcacgatc
     7981 cgcatggcgc ccgagtcgcg cacgctcgtc gccgacctcg agtcgctgcc gctggtcgtt
     8041 gctggtgcgc agaacgccgt gattcctctc ggacaggtcg cgcgcgtgag cccgtcgatc
     8101 ggtcccgcac gaatcgatca tctcaaccgc gatcgcgtga tcaccgtgga agccaacacg
     8161 cagggccgcc ccttgtcggc cgtcgtcggc gacacgatgg cgcgcgtcga tcaggccgtc
     8221 accttcccgg ccggctacgc catcagccag gggggtgaga ccgaggagca gcaggagatc
     8281 ttcggccaga tgttcgtcgc catcggggtg gcgatcatgc tgatgtactt cgttttggtg
     8341 ctgcagttcg ggtcgttcct cgagccgctc gcgatcctgc tgtcgctgcc cctgtcgctc
     8401 atcggcgtga tgctggcact gctcatcacc ggcaagccgc tcgagatcat gagcatgatc
     8461 ggcgtcatcc tgctcgtcgg catcgtggcg aagaacgcca tcctgctgat cgacttcgcc
     8521 aagtggtcgg aggaggaggg gctggaccgc cgcgaggcgc tcattcaggc gggacgcgtg
     8581 cggcttcgtc cgattctcat gaccaccttt gcgctcgttg ccggcatggt gccggtcgcg
     8641 ctcggcggcg gtgaaggggg cgacttccgc gcgccgctcg gcatcacggt catcggcggc
     8701 gtcgtcacgt ccacgctgct gacgctcctc gtcattccga ccgtctacga gatccttgcc
     8761 gacacgagag actggctggc cgcacgtctc ttccgctcga agggcaaggc cgtcccggcc
     8821 gccgttgaga gttgatgcca atgcacaacc cgttcgtgcc cgaatcgctc gaggggtgga
     8881 gcatcctcca tctcatgtac cgcgtgcgct gggatcggtt gaagcaggcg tcgcaggccg
     8941 acgtgcagcg gatggccgac gaggcggggc gtgccctgtc ggtgcccgac gcgggcgcca
     9001 ccgcggtcgc gcagatgctg gggcacaagg cggacctgat gttcatcgcc ttccggcgcg
     9061 gcttcgagca gctcgcccag gcacagctct cgctgtcgcg cgccgagctg tgcgcctacc
     9121 tcgagccgac gacgtcgtat gtgtcgatcg tcgagctcgg tatgtacgag atgacggcca
     9181 agatccacga gcagctcggt gcgctccaca aaccaggcag cgaggcgttc gaccgcgcct
     9241 tcgacgccga gatggagacg cagcggcagc gcgtgatgaa tcgcctcttc atcgaggtgc
     9301 cgaaatcgcg ctacgtctgc ttctacccga tgaacaaacg gcggggcgag accgtgaact
     9361 ggtattcgga atcgttcgag aagcgcgcgg cgatgatgcg tgagcacggc atgatcggac
     9421 gccactacac gggcaaggtc actcaggtca tttccgggtc gatcggatac gatgactggg
     9481 agtggggtgt cgatttgttc gcgcaggacc cgctcgtgtt caagaagctc atctacgaaa
     9541 tgcgcttcga cgaagcgagc gcgaagtacg cggagttcgg acctttctac acgggtttac
     9601 agttcgatct aacgaagctg agcgcatatc tcagcgggga aacgccttct ctggattaac
     9661 ccgaaatatg taatccaatg gggcggattc gtgagtacgg caatgaattc cgcattcgag
     9721 cttttgccta agtcttcacg gaacagcagg taaaaaggac ggcgtcggtc cggctggtga
     9781 ggcgcgggaa ttgctttcta ggcgcggagc gccaggagag actgtcaacc agccgagggc
     9841 ggagcccttg gctggtcttc cggctggaga ccacatgaac cctcaggaga tgttcgtcac
     9901 gcggttgcgc cggcaccgcc agcgcgcccg cgtctcgatc gaggcaatcg cggaaaacct
     9961 tcgcatccgc cccgaactca tcgaggcctt cgagaacaac gacctgtccg cgtggcctcg
    10021 cggactgtac gcgcgcgcgt ggattcgcac ctatgcgctc tctgtcgatc tcgatcccgt
    10081 cgatacgctg gaggagttct gcaggctctt tccgcagggc gatcgccgca cgggaggaac
    10141 gatccaggag attgcggcga ttgtggcgtc tccatcggag tatcgcgacg agttcaacca
    10201 tcccgtcggg cggcgcagca gcgatgaacg gagggccgag gcgccgagga cggcgtggca
    10261 cgcgttcttc acgcagcccg gccgagctct gtggagtcgg ctcaccgggt cgcagccggc
    10321 attccgcgat cgctccatca gttcgtagcg gtcgccggtc gttcggagtc acgacaacca
    10381 gctacccgct gccagctagc ggctaacggc cagtcgctat accataggcg cccatgagac
    10441 atcagaaact gctctgggcc gccattctgg tcgtttcgac cgcgtgggcc tgcaagtcgc
    10501 ctcaaccgca gccggcggcg gagcctgcgg cagcagcccc cgatcggggg cccgacgtca
    10561 tcttcgtccc gacgcctcaa gaggtcgtcg acgcgatgct gaagctggcc aaggtcacga
    10621 agaacgacgt gatctacgac cttggctccg gcgacggccg gattcccatc acggcggcaa
    10681 agacctacgg cgcacgcgcg accggcatcg acatcgatcc gcaacgcatc aaggaagcca
    10741 ccgaaaacct gaaggcggcc ggcgtcgggg accgggtcag gttcctcaat caggatctgt
    10801 tcacgacgaa catcagcgaa gcgacggtcg tgacgctgta tcttctgccc tcgctcaacg
    10861 tcaagctgct gccaaaactg aacaaggaac tgaagccggg gacccgcatc gtctcgcacg
    10921 ccttcgacat gagcgcggac ggcgtcgagc gcaagccact ggaaacagtg accgtcaacg
    10981 gccggtcgat ctatttctgg accattccca tcaagtaacg cccaaccccc aacgccccac
    11041 cgccttttgg gagtttggga attgggagtt tgggggtttc ccccggtttg atctagactg
    11101 agactccgtc tcagtttagc ggggagccga tcatggaaca ggcgttcgtc atcacattgc
    11161 gtgaagggct ggaggcgttt ctcatcgtgg ccatcagcct ggcctatctc cggaagaccg
    11221 ggcggccgca gctgataccc gccgtgcact ggggcatcgg cgtgtccatc ctggtcagca
    11281 tcggggctgc catcctgttc cagcaggcgt ccaatcaggc gttgtgggag ggggttctcg
    11341 cgctgacggc ggccgttctc gtcgggacgc tcatcgtcca catgtggcgt cacgcgaaac
    11401 gcatgaaagg cgagatcgaa tcgcgcctcg agtcaactgc gctgaagtcc gggacgaagg
    11461 cgctcgtcgg cgtattcctg ttcacgctgc tgatgatcac gcgcgagggg atggagacgg
    11521 cgctgctcat gggtacgctg gtcttccagg aagcggcctc gagcatcctc acaggcgcca
    11581 taggcggcac gctctgcgcg gcgtttgtgg cctggctctg gtcgcgttac ggacaccggg
    11641 tgaacctcgc gctgttcttc caggtcacgg ccatcttcct tggcatcttc gtcgtgcagc
    11701 tggcgatcta cgggctgcac gagctgacgg aggcgaacct cttccagggt agcgaggcgc
    11761 ttcactgggc caccgagcca tacgggccgg acggccgcta cggccagttc ctgacctatc
    11821 tgatggtcgc gctgccggtg gcgtggctct gcttcgccgc actcttcgga caccgtcccg
    11881 cccaccgctc tccacggatg gagcagccgg ccgcgagata gcccgataca gtactcaccg
    11941 tggggcgtag acagtatctc gactggctgc gcggcgtcgc tgtgttgatc atggtcgaaa
    12001 cgcacctggt cgatgcatgg acgcgcgcac ccgaccggtc agaggccgct tacaggtggg
    12061 cgatgttcgt cggaggattc agcgccccgc tctttctctt cctcgccggc atcgcgctgg
    12121 cgcttgcggc gggagcccgg acacgcaagg gggcacacct ctcggaagca gccgcgctcg
    12181 ccaggagaag agggtggcag attctcggtc tggcgttcct cttccgcctg cagtcgttcg
    12241 cgatcagcgg cggagtcttc ccgcaaacgc tgctgaaggt ggacatcctc aacgtgatgg
    12301 gtctcgggat gctgctggcc gcgctgttgt gggcgcgggg ccggcgcctg tcatcccgcg
    12361 cggcatggct ggcggccgtg gccgttctcg tggttttggc gacgccgatc gtgcgggcga
    12421 tggcgctgcc ctcctggctg ccctacccgc ttgcgtggta cctgcagccc gtgccggact
    12481 ccggggcgtt cacgctgttt ccatggctgg cgtttctcct gattggatcg gcagtcggcc
    12541 tgtggctcga tgccgcgggc accgggactg aagagcgccg cgtcgtcgta gctctctcgc
    12601 tggccgggcc tgcggtcgcg cttggagcgt acgcagcaac gttcctgccg tcgatgtacg
    12661 ccgggacgcc gtcctggagc gcctcaccgt cgatcttctt cctgcacctg ggcgtcgtga
    12721 tgacggccat ccctgccgcc tacgcgtggc gcaggcgggc gagcggctgg tcgccgctgc
    12781 gggagttcgg cgtggcgtcg ctcttcgtct actggatcca cgtggaaatg gtgtacggga
    12841 ttgtgagcct cccgctccat cgagcgctcg gcttcgagca ggcgatggcc gcctacgcgg
    12901 ccctgtgcgt ctttctgtac gcgctcgtca aactcaagga ccgcgggctt cgcaccctcg
    12961 caccttcgca ccctcgcacc ctcgcacctt agttcagcac gtcgaatatc cggctgactt
    13021 tcacgatgag gctgcgattc gggacacgcg ggcctagccc gagctcgtcg tcgatgaagt
    13081 gcgagtcggt gtagacgatg aagacgccgg cattggcctg ctgaagccag ccgaatcgaa
    13141 ggttggtcga ccagatgttg tcgcgatcgt tgtattgcac gagaccctgg acgaacactc
    13201 gtggggaaaa cgagtaggac acccgagtcc gccccaggtt cgtaacgaac tggccgtacg
    13261 gcaggttgac gtcgttccga tcccaggcca cttcggtatt gaagttctct ccaacacgca
    13321 ctcgaaactg aggtgtcagc ctgacacgat ggcccccgaa gaaccccccg gcgttcagct
    13381 ggagacggcc ggacaccggc gcgccctcgt tgctcgtgag cgagagctgc gcctccgtat
    13441 ggtcgtagac gccgaccggg acgacgacac cgggatagat cgaaaacggc tcgacaactc
    13501 cctcgtgcgt gaggttcact ccggtgctga actcccacgc gctcctgagt tcccacgccg
    13561 agtcgaggtg ccagaagccg gtctccagga tgccgtcgtg attccagtag gcgcggtagt
    13621 tggagtgagg cctgatctcc tgaaacttcg acgcctcccc cggccgcagg cgcgagaaca
    13681 cctggccttc gaccttccga aacccgcctt cacggctgag gaaacctact tccgggttga
    13741 agttccgccc ggtctccgtg taaccgccgc tgaggctcag cggctgcgtg tcgttgcggg
    13801 ccagaacctg aaatgcatgc tgactgtccg ggaatcccgg cgtcgctgtc cgggcaatga
    13861 agcccgagac gagccccgtt cgaccgattc cccaccggcc gtcggcggca tacgccaggt
    13921 tctgatcgtc gtcgtcggcg acgttgccgg tcgccagccg gcccacgaag atcgccccga
    13981 cactcgagcg cccctcgaag tcccgcctga cgcgtgccac agtgaagttg ttggccgcct
    14041 ggccgaaaac cgttcccgtc tgcatgttga gcaggcccac gttcaccgga cccgctcggc
    14101 ccgacagccg ggcgccgccg atgatcggga tctctcctcc gagctcgccg atgccgatgc
    14161 ggcggctgaa gaagacctcc gcttcgctcg ggctgccaac cgcgaagaga cccgcgttct
    14221 ccagaaagaa ggggcgcttt tcgggaaaga acaggttgaa gcgatcgaga ttgatttgct
    14281 gctcgtccac ttccacctgg gcaaagtcgg tgttgacggt ggcgtcgagc gtcaggctcg
    14341 gagttacgct gtacttcgcg tcaaggccga cgtcgccgaa gtacttggca cccgggttcg
    14401 acaaacctcc ctgccgcgcc tcgccaagcg cgtacggcac cagttggaga ttttgcggaa
    14461 ccggcaggtc gaggcccgtg aggactccgg ccatcgacac gcgcatgagg tcgaactgga
    14521 tcggaagcct cgaccaatat gccgtctctt tgcgccgtcg aatcgtccgc tggaagttca
    14581 cgccccattg gcgacctgtg cccggctcgt agcggagcgt gcggaacgga atggcgaact
    14641 cggcggacca tcccgactcg gaggtggtcg tccggacgtc ccagacgcca tcccagttca
    14701 ggttgaagcc gccgccggat ccgctggact gccgtccccc gccgccgcca ccacctccgc
    14761 ctcctccgcc aaaggggttg ttgttccctt ccccggttac ctggccgtcg tactcgagcg
    14821 cggccgggtt ggtggcgaag acgaagccgt tctggcgatc ccggtacgta tcgagcacca
    14881 tccggaagct gtcgctgtcg tcgagtggcg agtcgcggcg cgcgtccgag acgacgaggc
    14941 ctgacgggtc gcgatccgtc aacacgacgc cgacatacag cgtatcgtcg gtgtaaacga
    15001 tgcggacctc ggtcctttcc gacgacggct cgccggcgtc cggcgcggtc tgccagaact
    15061 cgacgagcgg ctcgaggttc atccagacgg ggtcatccga cactcggccg tcgagcaccg
    15121 gcccatccgt caccttcacc gctcgagcca cgggcgcgtc cggcaatgcc tgagcttggg
    15181 ccggaagcga ctgcacggag attagacaca aaaccagagg tatcgcccgc gcccggcgat
    15241 cccggcaaac catcactaac tcctagaggg cttgtgaatt agtggcaatc aggtttaccg
    15301 tagacgccga gctggcttcg atcgcaaacg gtcagaaacg ggttgagatt cggggcgaca
    15361 ggccgctaca attcgccact tcttccgtga gccaactccc cagacggaca aaaaacgaat
    15421 gatcaacgtg gccgctatcc ggatgcagca gttcggggtt cgattctatc aggcctcact
    15481 gaccgcagga gatatcgaca agctggtgcg cttcgaagtg ctcaactacg gagagtccgg
    15541 gcagcccgtc cgcggcggcg cgaaaaaagc gccgtcgaag gtgaactggg atctgctcga
    15601 acgccggatc gcgtcgagcg agagggccta ccagcgtcaa atcatcaggc gaaagatcga
    15661 cgaactcgtc cagtattacg agcagtgccg ccaggctcgc gaccttccgt cgattgccgg
    15721 cgcggtgatc atctcgtgcg acgagaagct gacgttcgag ccggtcgaag acggagcgtc
    15781 tatcgggcgc ctcaaagtgc ctgagcgcga gggcatcctg cgggccatcg acggccagca
    15841 ccggctgctg gcgttacacg cggacatcga caacatccgc gaggacgact tcaccgtgcc
    15901 ggcgatcatc ttcgaccgac tgccggaaga ccacgtcgtg cagatgttcg tgacgatcaa
    15961 cgccaagcac acgcgcctga acgcttcgca cctggtgagc ctctctggcc ggcagctcta
    16021 tcgcgacgac gccctcgcag cggcacacga tgtcgtccgc gggctgaacg atcgcgagga
    16081 ctcgccgctg caaggcgaga tcaagcttct cggcgtgggc cacggacgcg tggcccaagc
    16141 gccgctggcg caggagctga aaaagctgtt cgcgagcgat gcgttcgggg gcccccggaa
    16201 gtccgccgat ttccgcgagg acgcgaaacg gttcttcgtg aactatttca agcaggtttc
    16261 ggtcgtcttc agcgtcgcgt ggggcggcag gaagtacagc atcaaatcgg ccacggcgct
    16321 gcgcgcattc atccgggtgg cgcccgacgt ggtgcgcagg ctcgaccagg aacacgccga
    16381 ccgcagcgac ttccgggcaa tcggccgtgt catcgccccc tggggccgac gaatcgggaa
    16441 cctgcggttc gaaagcgaag gagtctggaa gcgggctgga accacggtgg attctcttgc
    16501 gcgcgagctg aagctggcgc tcgagtatcc cgagggtgcc ggggtctagc gaaaaaaata
    16561 caggacgggg cgctccgaag agtgccccat cctgaaatgc cagcctggct ccccccagca
    16621 tttacggcgc cctgccgtaa accacaggcc gacacctggg atattggcaa tggccgtgcc
    16681 gaacgacttt cttaggaaaa acggggactt ccccagcaaa cgtacccagg aatgtgggaa
    16741 gaattacacg cctgattttc acgtatgcgc attgatctga acgctgacgt tggcgagtcg
    16801 ttcggcgcgt acaagctcgg ccacgacgaa ttgctgatgg aggcagtcac gtcggcaaac
    16861 gtcgcctgtg gatttcacgc gggagacccc ggcgtcatgc gcgacacagt ggccatggca
    16921 cgtgagcaag gcgtcgccgt gggagcgcat cccgggtttc cagacctggc cggtttcggc
    16981 cgccgggaga tccacgccac gccgcgcgag atcgaggatc tcgtcgtcta ccagatcggc
    17041 gcgctggccg cggtggccgc cgcgcagggc gtgaggctgc agcatgtcaa ggcgcacggc
    17101 gccctgtaca acatggccgc gcgcgaaccg gcactggccg acgcgatcgc gcgcgcgacg
    17161 gcgagcgtcg atcgctcgct ggtgctcttc ggcctcgccg gctcgcggct catcgaagcg
    17221 gggcagcgtg ccggactccg tacagctcgc gaggcgttcg ccgaccgtgc gtatcggggc
    17281 gacggtgcgc ttgtgccacg cagccagccg ggcgcgatcg tggagactcc gaacgaggtc
    17341 gtgagccggg tcgtatcgat ggtcacccgg caggccataa cggcgatcga cgggtcgcag
    17401 gtgcgagtcg aggccgacac gatctgtgtg cacggggaca ctccaggggc agccaatctc
    17461 gcgaagcgaa tccggcactc cctggtcgaa gcaggcgtcg atgtcgtgtc agtgggatcg
    17521 acggcgggcg gcgtcagata gagcgctgct cccatttatg attagcgatt gttggtcttt
    17581 gattcgttga tgatttgtga ttcgagactg gtgattgcct ggccagtcgc caatcaacaa
    17641 tcatcaatcg acaacgatcc agcagtcacc tgtcaacacg cggcgtggca gccgagtgct
    17701 caccttttgg cacttcgccc gtcttaaccg caggaccgtg gacgtcagcg acgccgccct
    17761 catccagcgc agccggtcgg gcgacctgac ggcatttgac gcgctcatga cgcgctacca
    17821 agggctcgtc ttcaccgtgg cgtatgcctg cgcgcgcgat cgcgacgacg cgctcgatat
    17881 cagtcagaac gcgttcatga aggccttcga gcacctcgat tcgctccgcg acgaagccag
    17941 ctttcgttcc tggatcgccc gcatcgcgcg ccgcgagggg attgactggg cgcgtcgaaa
    18001 ccgccaccgg cgccagttcg tggatcccga ggaagtccac ttgcctgacg ccgcgccgag
    18061 ccaggaggcc cggctgctgt cggacgagtg cgggaggcgg ttggcggcgc ggctggaccg
    18121 gctgaaccgg cgctaccgcg tgaccgtgtc gctgcgctac gtcgagggga tgtcgattgc
    18181 cgagatcgcg gcggtgctcc gctgcagcga ggccatggtg aagaacatgt tgttcaggag
    18241 cctgcgcagg ctccggcagg agttgacgga agcgaggcag gtgccatgac ggtcgatcga
    18301 tgccagcggt ggtccgagca gtttctggcc ggcgagatga cggcgctcga acacgaggcg
    18361 tttcttcggc atgcgcggtc gtgtgaggca tgcaacagcc tcgtcctgct cgacgcggaa
    18421 ctgcgcgtag ctccaccggt ctcgaggctg acggcggcgg agggcgacgg cgtgcgccgc
    18481 tcggtgctgc ggcagcttca cgcgttgcag gagcggcgcc aggccgcagg ccagcggccg
    18541 cggggcgtgg ggaccgccct ccttgccgcc gcggccgtcc tccttgtggg cgtcggcatg
    18601 gtcgtggggc gcttctgggc cggcgcggca cgcggtgaag acggggaagc actcacgggc
    18661 ctcatcaagc aggcggcgct cggcagtctc actctggccg acgtcgagaa ctcgccttat
    18721 gtgttcacca acgtcgcctt tcgctccgtc gatgccgagc aggtggcagt gagtttcgac
    18781 gtcagcaggc acctcgagtt gacggcgcat cgggccgatc cgttgatccg gcagctggtc
    18841 gtacaggccg tggtcaaccc gtcgcccatc gggtctcgac tcaaggccat ttcctacgcg
    18901 gagggcgtcg ccgaccgcgc ggtgatggac gcgctgctga tcgccatgct cgacgacccg
    18961 agcgcagagg tccgcctgcg cgcgctcgcg gcgctggcgc ctcacaaggc cgaccccgaa
    19021 attcaatcgg cgttgctgac ggtgctgacg acatccgatt cagtgcctat gcgccttcag
    19081 gcgattgact acctggtcaa gggacgcgtg gacccgcagc ggctgcggag cgccgtgtcg
    19141 atgctcgacc ccgaggagca cgccgcattg ctggtcaggg cgagcggcta cctccagtga
    19201 ggactcagca catgacggac accatcgcgc gaaccgccgg cgtcatcctc gcggcgggtt
    19261 tgtcgctctc agcgcaaggg aaaacgttca cggacgaaat cagggtgtcg ccgggccagg
    19321 ccctcgaatt gaggctcgat acccgtgccg acgtgaccat caccggctcc gacgacgcat
    19381 gggtgcgggt gcaggcaatt ccgggcgatc agggtgccag tgacggcgtc gtcacgacgc
    19441 ggccgaccag ccgtggcgtc cagatcgtct cgaagggagc aggggagacg cgattcctca
    19501 ccggcaaccc ggcacggctc accatcgtcg tgcccaggcg attcgacgtg catatcacgt
    19561 caatggtcgg caacctgcgc gtggaaaacg tcgagggtca gattgatgga gaaacgaagg
    19621 gcggcaacct gacgctgacg gggctgagtg gcgggctcaa cctgacgacg agaggtggcg
    19681 acatccacgt gtcgaactct cggctcgacg gtaaggtcgt aaccatgggc ggcagcgtca
    19741 cactggaaga cgtcgccggc cacgtcaaca gcgtgtccat gggcggcagc gtcgtgtcgc
    19801 gcaccgcgcg gcgaaacggc ggagccgcgc cagggccgcc gggcgagctg cgcatcacga
    19861 cgacgggcgg cgcgatcgac gttgccgaag cgatggccgg ggcgatcgtg acgacgacgg
    19921 gcggcgccat ccacattcag caagcggccg gctatgtgag agcgaccacg actggcggcg
    19981 agatccgtct cgactcggtt gacggctgga tcgaggcgac gactcctggg gggggcgtca
    20041 tagcgacgat ggtgggcgat cccgaccgcg gcgatcggca tgtgaggctg gtatcggttg
    20101 gcggcgatat cacgcttacg gtgcccgagt cgctcggcat gcgcttcgac atcaccctgg
    20161 cctacacacg caacagccgg cagaactatg agatccactc ggatttcccg ctgcagatcc
    20221 ggcggaccga ctgggaatac gcgacgggcg atcgccggcg ccacatctat ggcaccggca
    20281 ccgccggcaa cgcccgccac ctcgtgcaca tcgaaactgt caacggtgac gtgaccgttc
    20341 gcaaggcgcc gtaaggagct gacgatgaag actgcgacgt ccattctgat catggcgctg
    20401 gctgtgccgt tccagtcgcc acagcccgca tcgtggaagg acccctcttc ccacgtgcgc
    20461 cgattcatcg acgtcgcgca gaacgtgcgg ctcgaagtgc ttgattgggg cggtgcgggc
    20521 cggccactgg tcctgctggc cgctgggggc gacacggcgc acgtcttcga cgacttcgcg
    20581 cccaagctca cgggcgactt tcatgtctat ggcatcacgc gccgcggctt cggcgagtcg
    20641 acctttgcgc cggtcacctc gggcgccgat acgttcggcg acgatgtgct cgccgtgctt
    20701 gatgcgctca agctggagaa gccggtactc gccggcagtt cgatcgccgg gcaggagttg
    20761 agctcgatcg gcacgcggcg cccggatcgc gtcgccgcgc tggtatacct cgatgcggcg
    20821 taccagtacg cgttcgacaa cggcaagatc ccgacgttcg aagacatcca ggcggttggt
    20881 tttccccagc gtccgccgcc gaccgacgac gatctggcca ccttcgatgg gctgcgccag
    20941 tactacaccc ggctgctggg attcacgtac ccggaggcag aacttcgtca aaagcgtttg
    21001 gttacggagc aaggtggagt cggggcggag cgggtgtttc cgggcggccg gagcttgcta
    21061 atcaccatga cgaagttcgt tgagattcga accccggcgc tgtttctcgt gagtggccag
    21121 aacccgggcc gatgggcgaa tagcagcacc gatccgaaag tccgccagca ggtcgcggga
    21181 ttgcaggccg tcctcgcgcg gcaggcaaag cagattgagg acagcatccc cagcgcacga
    21241 gtcgtacaac tgccggacgc gaatcactac gtgttcctgt cgaatgaggc ggacgtgctc
    21301 cgcgagatgc gcgcgtttct tggccgccta cgctagagaa tggcaagccg gttcgatgtg
    21361 acgatccgct actacttgcc gcgcgagccg atcatcgcgg actcgtggac gtcgcgacct
    21421 gcctctagta gtgtcgcgca gcgtcccctt ccgcagccag gacgcgccgc aagactttgc
    21481 cgaccatcgt cttcggcagc tccttgcgga agtccacctt cgccggcacc ttgtaagggg
    21541 cgagacgctc gcggcagaac gcgcggatct cgtcctcgct tgccgactga ccgtgcttga
    21601 gcaccaccca ggccttggcc acctcgccct tgagctcgtc ggggattccg gcgacgccca
    21661 cttcgagcac ggcggggtgg gcggagatga cctcctcgat ctcccgcggc cacacctgga
    21721 agccgctggt cttgagcatg tctttcttcc gatcgaccag gaacacgtag ccgtcccggt
    21781 ccatgtacgc cagatcgccg gtgtggagcc acacgcggcc gtcctctcgg gttcgcagcg
    21841 tctgggacgt ttcgtccggg ttgtcccagt actcggacat gtactgctcg gcgcacatga
    21901 tcagctcacc cacttctcct gtgggcatgt cggccgtgcc agtctcggcc tcgacgatgc
    21961 gaacctcgac atcgggaacc gggatgccga tcgatccgat cttgttcggg cctgcgaccg
    22021 ggttcaccag gcaggccatc atgccctcgg tgagcgagta gccctcgatg atcctggcgc
    22081 cggtcttctc ctcgaactgg cgcttggtct cggccatgag cgcggcggaa ccggagaagc
    22141 acagcctgat cgagctgagg tcgaccttgc ccgcgcggac ctccgggtga ttgagcatag
    22201 ccgtgtacag cgtcgggacg ccgttgaaga cgaccggcct cacctgcttg atcgtcttga
    22261 gcagatcgtc gatgtctcgc ggattcggca cgagcgacag cgggttgggg cccagcagtg
    22321 cgagcggctg cacgccgacg ttcgcgtaca cgtggaacaa cggcaacggc agcatgatgg
    22381 cgtcccaggg gcgcttggct gacttggtcc actcgtacag ctggcggcct gccgcgacgt
    22441 agtgccgatg cagaccgacc acgcctttag gcgtgccggt cgtgcctccg ctggcgagaa
    22501 tgacggcgcg gtcgttcggc ccgaccttcg ccgtcggccg cggcgaggcg ctgtgagacc
    22561 gcagcagatc gctcatccac agatcgcctg gcgcgatgga aatgcggtgg ccgtccttct
    22621 tctccttgag cagcgtgaac agcacccgca atacgggcgg caggtaatcc ttgatggacg
    22681 ttgcgatgac gtgacgaacg ctggtcctgg gccgtgcacg gttcaggcga tcgtaaaagg
    22741 cggtcagcgt gaccacggtg tgaacatccg acgaagtcag cgactgctca atctcccgtt
    22801 cggtgtaggt gggattgacc gggacaacga ttgcgcccgc tttccaggct ccgtactggg
    22861 cgatcagaaa ctgggggcaa ttcggcagga tgagcgccac gcgatcgccc ttgcgaacgc
    22921 cgaggcccga gagcgccgcg gcaaacgcgg tgctctcctt gtcgaggcgc ccgtaggtga
    22981 cggtcgtccc cttgaagaac agggcgggct tgtcggcgtt gtcgcgcgcg agctcttcca
    23041 gatagtcgag gagagtgcgc tcgggatacg gagccagcga ctgcgggacg
//
LOCUS       DBNGSH010000306         2521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_703926, whole genome shotgun sequence.
ACCESSION   DBNGSH010000306 DBNGSH010000000
VERSION     DBNGSH010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_703926"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="AC16CY_07220"
     CDS             complement(<1..203)
                     /locus_tag="AC16CY_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR3353611.1"
                     /translation="MRHAAVILLPAFTLVLAGCAARAKAPSPSAPASPAILEVRQGLA
                     SYYGPGFDGRVTASGARFDMKAMV"
     gene            382..>2521
                     /locus_tag="AC16CY_07225"
     CDS             382..>2521
                     /locus_tag="AC16CY_07225"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR3353612.1"
                     /translation="MIARIRIKRLPAVFSRYPSLFVVAILALCLGVWSGIFGSIWYLR
                     GIVVSVPDTEQLRGIGSMAQATSLFDRTDKPAFTIFKEQRIDVPLARVSPNLIDAIIA
                     IEDQRFYDHNGVDVVRVAGAALSNVREGRRAQGGSTITQQLARQSFLTPDKTIRRKLT
                     EVVVAARLEEQFTKDEILELYLNKVYFGDGLYGVEAASLGYFGKSASEVSVAQAALLA
                     GLVKSPSTYAPTVSTERAKARRDLVLRAMLDTKAIDKAAYDSALATPVTLDDALRRGE
                     AFGQYFKEEVRRFLVQRFGWERVYQGGLKVFTTVDLDMQKAAESEVMRALKEIEGRQG
                     KKRNEQDAADPLQAALVAIDPATGEVRAMVGGRDFEKSNFNRATQAKRQPGSAFKPFV
                     YAAALERGFSPATLITNLNDPIMTLQGEWVPEDDHNDEGPSMTMRTALRTSSNRAAVR
                     MLENVGIPVAVQYAKQLGFGSVPSVPSLALGSGEVTVSSMTAAYAAFANHGQVLAPVL
                     VRRVETTEGEVLYKAEQKPQQGVSESTAFLMTTMMADVINAGTGAAARSVGFTLPAAG
                     KTGTTNDYHDAWFVGFTPRLAAGVWIGYDQPRTIIGRGYASVLAVPLWGRFMAAATRK
                     DAPEWFDEPKTIATATICRLSGKLATDGCREAVIADASGNITRGSSVHSEYFVRGTEP
                     TEACPLHQRILASPLRAIASLFGGPSKPSSQ"
BASE COUNT          438 a          834 c          860 g          389 t
ORIGIN      
        1 accatcgcct tcatgtcgaa ccgcgcgccg cttgcggtga cgcggccgtc gaatcctggt
       61 ccgtagtagg aggccagtcc ctgacgaact tcgaggattg ccgggctggc gggcgcagat
      121 ggcgacggcg ctttcgcgcg ggccgcgcag cccgccagga cgagtgtgaa tgccggcagc
      181 aggatgaccg ccgcatgcct catgatttat cgtctcatgc accctcgcgc cctcgcacct
      241 cacacctttg cgcccttact cgaccgtgca aagttgacac ctcgacggta accgcaagtc
      301 gggtatcctg agttggtacc cgcgcgccaa cccggcgtgc gggcggcaag ccgtggcacg
      361 gtcgttgcca gaggatgacg cgtgatcgca cgaatcagaa tcaagcgact ccccgccgtg
      421 ttcagccgct acccgtcgct ctttgtcgtg gcgatcctgg cgctctgtct cggcgtctgg
      481 agcgggatct tcggctccat ctggtacctc cgcggcatcg tcgtctcggt gccggatacc
      541 gagcagctgc gcggcatcgg cagcatggcg caggccacct cgctgttcga tcgcacggac
      601 aagcccgcat tcacgatttt caaggaacag cgcatcgacg tgccgctcgc gcgcgtgtcg
      661 ccgaacctga tcgacgcgat catcgcgatc gaggatcagc gcttctacga tcacaacggc
      721 gtggacgtcg tgcgcgtggc gggcgccgcg ctcagcaacg tccgtgaagg ccggcgcgcg
      781 cagggcggaa gcacgatcac gcagcagctg gcgcgtcaga gctttctcac gcccgacaag
      841 accatccgcc ggaagctgac ggaggtcgtc gttgcggccc gcctggaaga gcagttcacg
      901 aaggatgaaa tcctcgagct gtacctgaac aaggtgtact tcggcgacgg gctctacggg
      961 gtggaggcgg cctcgctcgg ctacttcggc aaatccgcgt cggaggtcag cgtggcacaa
     1021 gcggcgctgc tggccgggct cgtcaagtcg ccgtccacgt acgcaccgac ggtcagcacc
     1081 gagcgggcga aagcccgccg cgacctggtt ctgagggcca tgctcgacac gaaggcgatc
     1141 gacaaggccg cctacgacag cgccctggcc acccccgtga cgctcgacga tgcgctcagg
     1201 cggggggaag ccttcggcca gtacttcaag gaggaggtca ggcgcttcct cgtccagcgc
     1261 ttcggatggg agcgcgtgta ccagggcggg ttgaaggtct ttaccaccgt cgatctcgac
     1321 atgcagaagg cggccgagag cgaggtgatg cgcgcgctga aggaaatcga gggtcgccag
     1381 gggaagaagc ggaacgagca ggacgccgcc gacccgctgc aggcggcgct cgtggcgatc
     1441 gaccccgcca ccggggaggt gcgggcgatg gtcggcgggc gcgacttcga gaagagcaac
     1501 ttcaaccgcg cgacgcaggc caagcgccag cccggctcgg cgttcaagcc ctttgtctac
     1561 gccgcggcgc tcgagcgcgg attctcaccg gcgacgctca tcaccaacct caacgatccg
     1621 atcatgacgt tgcagggcga gtgggtgccc gaggacgacc acaacgacga gggcccctcg
     1681 atgacgatga ggacggcgct gaggacgtcc agcaatcgcg ccgcggtccg catgctcgag
     1741 aacgtcggga tccccgtggc cgttcagtac gcgaagcagc tcggcttcgg ctcggtgccg
     1801 agcgtgccgt cgctggcgct gggatccggc gaggtcacgg tatcgtcgat gacggcggcc
     1861 tacgccgcgt tcgccaacca cggccaggtg ctggcgccgg tgctcgtccg ccgcgtcgag
     1921 acgaccgaag gcgaagtgct ctacaaagcg gagcagaagc cgcagcaggg cgtcagcgag
     1981 agcacggcgt ttctgatgac gacgatgatg gccgacgtca tcaatgccgg cacgggcgcc
     2041 gcggcgcgga gtgtcggctt caccctcccc gcggcgggga aaacgggaac gaccaacgac
     2101 tatcacgacg cgtggttcgt cgggttcacg ccgcggctcg ccgccggcgt gtggatcgga
     2161 tacgaccagc cccgcaccat catcgggaga gggtacgcgt cggtgctcgc ggtgccgctg
     2221 tgggggcgct tcatggcggc cgccacgcga aaggacgctc ccgagtggtt cgacgagccc
     2281 aagacgatcg cgacggcgac catctgccgc ctgagcggga agctggcgac cgacgggtgt
     2341 cgtgaagccg tcatcgccga cgcgtccggg aacatcacgc gcggatcgag cgttcacagc
     2401 gagtatttcg tgcgcggcac cgagcccacc gaggcgtgcc cgcttcatca gcgaatcctc
     2461 gcctcgccgt tgagggccat tgcctcgcta ttcggcggtc cgtcgaagcc ctcgtcccag
     2521 t
//
LOCUS       DBNGSH010000307         6282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2571271, whole genome shotgun sequence.
ACCESSION   DBNGSH010000307 DBNGSH010000000
VERSION     DBNGSH010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2571271"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="AC16CY_07230"
     CDS             complement(<1..503)
                     /locus_tag="AC16CY_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353613.1"
                     /translation="MAQAIVFYISGHGFGHASRSIEVINAIFAQRPETRVGVRTSAPR
                     WLFDLTVKGKITYSTLECDTGVVQSDALTLDEADSIRRAAAFHSDLVTRAASETRSLR
                     ELGAGLIVGDIPPLAFAVGASAGIPSIGLGNFTWDWIYADYPRVRLAPSLLPAIRAAY
                     AKASMALR"
     gene            complement(556..2940)
                     /gene="lon"
                     /locus_tag="AC16CY_07235"
     CDS             complement(556..2940)
                     /gene="lon"
                     /locus_tag="AC16CY_07235"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3353614.1"
                     /translation="MPDTAPQLPPELPVLPLRRTVALPLTLQPLAVNRPVSAETVNRA
                     LGSDRLICLILQRTDADDPTPDDLHRVGTVGVIRQMAKTGGGMNIIVEGLARVRADLI
                     TRAGTSMRAQITPLPESFDRTIEAEAHIRRIQELIERALSIATGLSEDLRGVVMNIDD
                     PLRLAYVLATLIDMKPSDKQVMLEENDLLKKLEAVSAALTREVSLLELKGKIESQAQQ
                     EMTDAQRQYFLRQQLKAIQQELGEGEGNELAELRKRIEDAKLPEATNTVALREVDRLE
                     RMTAASPEYQMLRTYIDWLLDVPWSTTTVDRIDPVEARGALDEDHYDLDKVKERIVEY
                     LCVRKLKGDMKGPILCFVGPPGVGKTSLGQSIARAMSRKFVRVSLGGVRDEAEIRGHR
                     RTYIGSIPGRIVQALKQAGSMNPVFMLDEIDKVSVGFQGDPAAALLEVLDPAQNHSFR
                     DHYLEIPVDLSRVLFIATANQLGTVHPALLDRMEVIQLAGYSEEEKLHIARKYLLPRQ
                     MSEHGLQPGTMEITDNAMRLVISEYTHEAGVRNLERQLGTLARKVAASIATRPQDAPT
                     PPTTVVDRDQVDDYLGPARFKKELAFRTSRPGVATGLAWTEAGGDVLFVEATLLPGGN
                     QNIILTGQLGNVMQESARAALSHIRANADRLGISPEFLAKQDLHVHVPAGAIPKDGPS
                     AGVTMATAILSAARNEPVRRDVAMTGEITLSGLVLPVGGIREKALAARRSGIKTVILP
                     ALNQPDLVELAEEIRKDMTFVPVETLEQVVEVALSNGQAPRLSGPEVAQPTVTG"
     gene            3075..3398
                     /locus_tag="AC16CY_07240"
     CDS             3075..3398
                     /locus_tag="AC16CY_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353615.1"
                     /translation="MWYASRVDERQFFTEKPEQRPARFQCPRCRRTNDYSVRWMRRTK
                     KPQIPSGADERDRALFGKVKDHLIRVDDDLVCKTCGKRFEIPSHRALVFLEELEGLPQ
                     EDYDE"
     gene            3464..5998
                     /locus_tag="AC16CY_07245"
     CDS             3464..5998
                     /locus_tag="AC16CY_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3353616.1"
                     /translation="MTRSDEALVNIRRACVALLLLLVAGTVPSAQAPSGQRQMAPAAS
                     AALRALNLGQFDEVDRVLGTAADPTSVAIRARARIARGRYAEAEMLLAPPGAAQPASD
                     AALELGLLEIHIGRRAEGTRRLRLLVNRLDARTAADYFRLARAARALATFEDAQAFHD
                     ANGYFRDANRLAPDDADINAEWGALFLDKHNPADAMKSFQEALRVDASHVGALIGTAA
                     AIAGENPPAARAALERAIKTNPNSVAAHLLAAELALDDRKRDDARASIQKALSINPNS
                     LEARSLEAAMALLEDRTADFEKRVQDILTINPIHGEVYRMAGDHLARNYRFDEAVVQV
                     RRAIALDPQNPRAYADLGTHLLRTGDEPGARTALDRAFKADPFDQTTFNQLDLLDTLD
                     KFVTITDGDIIMRLHPDEVGVMREHAMPLAKQALETLQRLYEFKVTGPILIEMFPKHD
                     DFAVRTIGLPGFIGALGACFGRVVTLDSPRARDPGEFSWSETLWHEIAHVITLQMSKN
                     RLPRWLSEGVSVWEERRARPEWGREMDLSFAQALNEEKILKLQVLNEGFSDPRLISLS
                     YYQASLVVDHIVETYGEPALRRLVRAYGDGLETEAAVKQALGVGLDQVQTGFDARLKK
                     QYASLLQALQTPKIGPKPALDDLKKLAEANPESFAVQMQLGLSLHENKDPKSAIQAFE
                     RASRLAPTASGDNNPNKLIAAVALEMKDEPRAARALEALLATDSADVEAARQLAKLVE
                     PLGDARRTENAYRRIAEADPFDRDAQTALGRLALRRKDTESAMRAFRTALAANPPDRA
                     QAHVDLAEAYLAAGRRAQAKSETLAALEIAPSFERAQDLLLTLVEP"
     gene            complement(6098..>6282)
                     /locus_tag="AC16CY_07250"
     CDS             complement(6098..>6282)
                     /locus_tag="AC16CY_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353617.1"
                     /translation="IWLVIQFLSGVGSLATASSEEGAGGIAFWAHVAGFAAGLIGVFV
                     FRRPKPQWDELDRYGR"
BASE COUNT         1048 a         2134 c         2059 g         1041 t
ORIGIN      
        1 cgaagcgcca tcgaggcttt cgcgtaggcc gcgcggattg ccggcaggag cgacggcgcg
       61 agacgcaccc gcgggtagtc ggcgtagatc cagtcccacg tgaagttgcc gagcccgatg
      121 gacgggatgc ccgccgaggc gccgacggcg aaggccagcg gggggatgtc gcccacgatg
      181 agccccgcgc ccagctcgcg gagcgaccgc gtttcgctgg ccgcgcgggt gaccaggtcg
      241 ctgtgaaatg cggccgcccg ccggatgctg tctgcctcgt ccagcgtgag cgcatcggac
      301 tgcacgacgc cggtgtcgca ctcgagcgtg ctgtaggtga ttttgccctt cacggtgagg
      361 tcgaacagcc agcgcggagc ggacgtgcga acgccgacgc gtgtttctgg gcgctgcgcg
      421 aaaatggcgt tgataacctc gatcgaccgt gaggcgtgac cgaacccgtg acctgaaatg
      481 tagaagacga tcgcttgtgc caaagacggc ctacgcgtta gtgggtccgc cgcaaggcgg
      541 cccggtgagc catggtcagc cggtcaccgt gggctgagcg acttccggtc cgctcaaccg
      601 gggtgcctga ccgttcgaga gtgcgacttc gacgacctgc tccaatgttt cgaccggcac
      661 gaaggtcatg tcctttcgaa tctcctcggc caactcgacc aggtctggct ggttgagcgc
      721 cgggagaatt accgtcttga tcccggacct tcgcgccgcc agcgcctttt cgcggatgcc
      781 gccgaccggc agcaccagcc cgctcaacgt gatctccccg gtcatcgcca cgtcccgccg
      841 gaccggctcg ttgcgcgcgg ccgacaggat cgccgtggcc atggtgaccc cggccgacgg
      901 tccgtccttg gggatggcgc cggcaggcac gtgcacgtgc aggtcctgct tggcgaggaa
      961 ttccggcgaa atccccagac gatcggcatt tgcccgtatg tggctgaggg ccgcgcgcgc
     1021 cgactcctgc atcacgtttc ccagctggcc ggtcaagata atgttctggt ttccgcccgg
     1081 aagcaaggtc gcctcgacaa acaggacgtc gcctccggct tcggtccacg caagacccgt
     1141 ggctacccct ggtctcgacg tgcggaacgc cagctctttc ttgaaacgcg ccggcccgag
     1201 gtaatcgtcg acctgatccc ggtcgacgac ggttgtcggc ggcgtcgggg cgtcctgggg
     1261 gcgcgtcgcg atgctggccg ccaccttccg ggccagcgtg ccgagctgcc gctccaggtt
     1321 gcggaccccg gcttcgtgtg tgtattccga gatcaccagc cgcatggcgt tgtcggtgat
     1381 ctccatggtg cccggctgca ggccatgctc gctcatctgc ctcggcagca ggtactttcg
     1441 ggcgatgtga agcttctctt cctcgctgta tccggcaagc tggatgactt ccattcgatc
     1501 gagcagcgcc ggatgaacgg tcccgagctg attggccgtc gcgatgaaca gcacacgcga
     1561 caggtcaaca gggatctcga ggtagtgatc gcggaacgag tggttctgcg cggggtcgag
     1621 cacttcgagc agcgcggccg ccggatcgcc ctgaaagccg accgagacct tgtcgatctc
     1681 gtcgagcatg aacaccgggt tcatcgagcc ggcctgcttg agcgcctgga cgatgcgccc
     1741 ggggatggag ccgatgtagg tccgccggtg cccgcggatc tccgcctcgt cgcggacccc
     1801 gccgagcgac acccgcacga acttccgtga catggcgcgg gcgatcgact gtccgagcga
     1861 ggtcttgccg acgccgggcg gccccacgaa gcagaggatg gggcccttca tgtcgccctt
     1921 cagcttccgg acgcagaggt actcgacgat ccgctctttc accttgtcga ggtcgtagtg
     1981 gtcttcgtcg agggccccgc gtgcctcgac cgggtcgatg cggtccaccg tcgtcgtgga
     2041 ccacggcaca tcgagcagcc agtcgatgta ggtacggagc atctggtact ccggtgaggc
     2101 ggccgtcatc cgctccagac ggtccacttc gcgcaaggcg acggtgttgg ttgcctcggg
     2161 gagcttggcg tcctcgatcc gcttccgcag ctcggccagc tcgtttcctt caccttcacc
     2221 gagttcctgc tgaatcgcct tgagctgctg gcgcaggaag tactggcgct gagcatcggt
     2281 catctcctgc tgcgcctgcg actcgatctt ccccttgagt tcgagcagcg agacctcacg
     2341 cgtcagggct gccgacacgg cctccagctt cttcagcaga tcgttctcct cgagcatgac
     2401 ctgcttgtcc gacggtttca tgtcgatgag cgtcgccagc acatacgcca gcctcagcgg
     2461 atcgtcgatg ttcatcacga cgccgcggag gtcctcggag agccctgtcg cgatcgacag
     2521 cgccctctcg atgagctcct ggatccggcg gatgtgcgcc tcggcctcga tcgtgcgatc
     2581 gaagctttcg gggagcggcg tgatctgcgc ccgcatcgag gtacccgcgc gcgtgatcag
     2641 atccgcgcgc acgcgggcga gcccctcgac gatgatgttc atgccgccgc cggtcttggc
     2701 catctgccgg atcacgccga cagtcccgac gcggtggaga tcgtccggag tcggatcgtc
     2761 ggcgtcggtt cgctgcagga tgaggcagat cagccgatcc gagccgaggg ccctgttcac
     2821 cgtttccgcg gacactggcc ggttgaccgc cagcggctgc agcgtcagcg gcagcgcgac
     2881 ggtacggcgc aggggcagaa ccggcagttc gggcggcagt tgtggagcag tatcgggcat
     2941 tgtgtcaggc cccagggccc aggcctcagg cctcagggct gggccaggcc tcaggcctac
     3001 gacctgagac gccctgtggc ctgagccctg attcctgagg cctaccgtgt ggtccagtct
     3061 atcagtgctt gtccgtgtgg tacgcttcgc gcgtggacga gcgccagttc ttcaccgaga
     3121 agccggaaca gcggcctgcg cggtttcagt gcccgcgatg ccgccgcacc aacgactatt
     3181 cggtcaggtg gatgcggcgc accaagaagc cccagattcc ctccggcgcc gacgaacgcg
     3241 atcgggccct gttcggcaag gtcaaggatc acctgattcg cgtcgacgac gacctcgtct
     3301 gtaagacgtg cggcaagcgc ttcgagatcc cctcgcaccg ggcgctcgtc ttcctcgagg
     3361 agctagaggg gctgcctcag gaggactacg acgagtaggc agtaggcagt gggcgcatcc
     3421 actatactga caacgatccc gtgtgatgtc tcttgagcgg cacatgaccc gttcggatga
     3481 agcgctcgtg aacattcggc gtgcgtgcgt ggccctcttg ctcctgctcg ttgcggggac
     3541 ggtaccctcg gcgcaggcgc cgtccggaca gcggcagatg gcgccggctg cctccgcggc
     3601 gttgcgcgcg ctcaatctcg gccagttcga cgaagtcgat cgagtgctcg ggactgcggc
     3661 cgatcccacg tcggtcgcca tccgtgcccg cgcccgcatt gcccggggcc gatacgccga
     3721 ggccgagatg cttctggcgc cgcccggcgc ggcgcagccg gcgagcgacg ccgccctcga
     3781 gctcggtttg ctggagatcc acatcggccg gcgcgcggaa ggcactcggc ggctgcggct
     3841 gctcgtcaat cggctcgatg ccagaactgc cgccgactac ttccgcctcg cgcgcgcggc
     3901 ccgcgccctg gcgaccttcg aggatgccca ggcgttccat gacgccaacg gctacttccg
     3961 cgacgccaac aggctggcac ccgacgacgc ggacatcaac gccgagtggg gagcgctctt
     4021 cctcgacaag cacaatcctg ccgatgcgat gaagtcgttt caggaggcgc ttcgcgtaga
     4081 cgcgagccac gtcggagccc tcatcggcac cgcggcggcg atcgcgggcg agaatcctcc
     4141 ggcggcgcgc gcagccctcg agcgcgccat caagaccaat ccgaactcgg tggccgccca
     4201 tctgctggcc gccgagctcg ccctcgacga tcgcaagcgc gacgacgcac gggccagtat
     4261 ccagaaggca ctctcgatca acccgaacag cctcgaagcc cgatcactag aggcggcgat
     4321 ggcccttctc gaggaccgaa cggccgattt cgagaagcgc gttcaggaca tactgacgat
     4381 caatccgatc cacggcgagg tgtatcgcat ggccggcgat catctggccc gcaactaccg
     4441 cttcgacgaa gcggtcgtac aggtccggcg cgccatcgcg ctcgatcccc agaatccgcg
     4501 ggcgtatgcc gatcttggaa cgcacctgct gcgaacgggc gacgagccgg gcgcgcgcac
     4561 cgcgctcgat cgcgccttca aggcggatcc cttcgatcag acgacgttca accagctcga
     4621 cctgctcgac acgctcgaca agttcgtcac gatcaccgac ggcgacatca tcatgcgcct
     4681 gcaccccgac gaagtggggg tgatgcggga gcatgcgatg ccgctggcca agcaggcgct
     4741 cgagacgctg cagcgcctct acgagttcaa ggtcacgggg ccgattctca tcgagatgtt
     4801 ccccaagcac gacgacttcg ccgtccgcac gatcggcctg cccggcttca tcggtgccct
     4861 cggggcgtgc ttcggccgcg tggtgacgct cgactctccg agggcgcgcg accctggcga
     4921 attcagctgg agcgaaacac tctggcacga gatcgcccac gtcatcacgc tgcagatgtc
     4981 gaagaaccgg ctgccccgct ggctgagcga gggcgtctcg gtgtgggaag agcggcgggc
     5041 ccggcccgaa tggggccgcg agatggacct gtcgttcgcg caggcgctga acgaagagaa
     5101 gatcctgaag ctccaggtgc tcaacgaggg gttcagcgac ccgcgcctga tctcgctgtc
     5161 ctactaccag gcctcgctcg tcgtcgatca catcgtggag acctacggcg agcccgcgct
     5221 ccggcggctc gtccgggcct acggcgacgg gctcgagacc gaggcggccg tgaagcaggc
     5281 gctcggcgtc gggctcgatc aggtccagac cggcttcgac gcgcgcctca agaagcagta
     5341 cgcgagcctg ctgcaggcgc tgcagacgcc caagatcggc cccaagcccg cgctcgacga
     5401 cctgaagaag ctggccgagg ccaaccccga gagcttcgcg gtgcagatgc agctcgggct
     5461 gtcgctgcac gaaaacaagg atcccaagtc ggcgattcag gcgttcgagc gtgcctcccg
     5521 cctggcaccg accgcgtcgg gcgacaacaa cccgaacaag ctgattgcgg ccgtcgcgct
     5581 cgaaatgaag gacgagccgc gcgcggctcg agcgctcgag gcgctgctcg cgaccgacag
     5641 cgccgacgtc gaagcggcgc ggcagctcgc caagctggtc gagccgctgg gcgacgcgcg
     5701 ccgcaccgag aacgcgtacc ggcgtatagc ggaagccgat cctttcgatc gcgacgccca
     5761 gacggcgctc ggccggctgg cgttgcggcg caaggatacc gagagcgcca tgcgtgcgtt
     5821 ccgcaccgcc ctggcggcca atccgccgga ccgcgcccag gcgcacgtcg accttgccga
     5881 agcctacctt gccgcgggac gaagggctca ggcgaagtcg gagacgctcg cggcgctcga
     5941 gattgctccc tcattcgagc gtgcgcagga tctgctcctg acgctcgtcg aaccctgagc
     6001 cacgcgatca agaaaacgcc agacacgtac gtcaaccaca gaggctcaga gaacgcagag
     6061 aaatccaacc agctctgtgt tctccgtgtc tctgtggtta acgtccgtat cggtcgagtt
     6121 catcccactg aggcttcggc cgtctgaaca cgaagacgcc gatcaagccg gccgcgaagc
     6181 ccgcgacgtg cgcccagaac gcgatgcccc cggcgccctc ttcgctcgag gcagtggcaa
     6241 gcgagccgac cccgcttaga aactggatca cgagccagat cc
//
LOCUS       DBNGSH010000308         1626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1552882, whole genome shotgun sequence.
ACCESSION   DBNGSH010000308 DBNGSH010000000
VERSION     DBNGSH010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1552882"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="AC16CY_07255"
     CDS             <1..866
                     /locus_tag="AC16CY_07255"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3353618.1"
                     /translation="ETWTAVRTETGGRVEAAVLPQNNNIPGSDPAALKMLVAGEIHFF
                     TLMGGILGTVVPAAEVQQVPFAFRSAAQAHETMDGGLGAYLRAEMAAAGIQGFAVGAF
                     DNGMRQIAGSKPIRLPSDLSGIRMRVPAGQMVADTFRALGAEPITINSSAIYEALKSG
                     KVDAQENPLALIDNFKLYEVVKYVSMTNHMWSGFNQLAHLLTWRRLPDDIRGAIDRNV
                     ARYVRLQRQDQERANARLRTELAKRGLVFNDVDAAPFRRQLTGFYADWKKRLGTRCWS
                     LLEAAAGSSLA"
     gene            complement(883..>1626)
                     /locus_tag="AC16CY_07260"
     CDS             complement(883..>1626)
                     /locus_tag="AC16CY_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353619.1"
                     /translation="LSNLDTVDNPNATGNSLNMVKREFDNRTSLITDPADGRLPALTP
                     EGLRRQRANTQALLTTPWQVGADTASPAPQQRLPNGAADLSNMLRCMSWGVPKIGGNT
                     NYTSHHQIFQGPGYVVFLSEVNHEARVIPLDGRPHLPSGIRQWNGDSRGRWEGHTLVV
                     ETTNFSPGSYFMGSADRLHLTERFTRVGPDRIDYAITVDDPTTWTKPWTAVVRLTRDD
                     GDRVHEFACHEGNYEVVRGILGGARAEDK"
BASE COUNT          273 a          509 c          548 g          296 t
ORIGIN      
        1 tggagacgtg gacggccgtc aggaccgaga ccggcggacg agtcgaggcc gcggtcctgc
       61 cgcagaacaa caacatcccc ggaagcgatc cggctgcgtt gaagatgctg gttgccggcg
      121 agattcactt cttcacgttg atgggcggca ttctcggcac cgtcgtgccg gccgccgagg
      181 tccagcaggt gccgttcgcg ttccgatcgg cggcgcaggc gcacgagacg atggacggcg
      241 ggctgggtgc gtatcttcgc gcggagatgg ctgccgccgg gattcaggga ttcgccgttg
      301 gcgctttcga caatgggatg cggcagatcg cgggcagcaa gcccattcgg cttccctcgg
      361 acctgtccgg gatcaggatg cgtgtgccgg ccggacagat ggtggccgac acattcaggg
      421 cgcttggtgc ggaaccgatc acgatcaaca gcagcgcgat ctacgaggcg ctcaagtcag
      481 ggaaggtcga cgcccaggag aatccactcg ccctcatcga caatttcaag ctgtacgagg
      541 tcgtgaagta cgtgagcatg acgaaccaca tgtggtcggg gttcaaccag cttgcgcatc
      601 ttctgacatg gcgccgcctg cctgacgaca tccgtggggc gatcgatcgc aacgtcgcgc
      661 ggtatgtccg gctgcagcgg caggatcagg agcgcgcgaa cgcccgtctg cggacggagc
      721 tggcaaagcg aggactcgtc ttcaacgacg tggacgccgc ccccttccgc cgccagttga
      781 ccggcttcta cgccgactgg aagaagaggc tcggcaccag gtgctggtcg ctgctggaag
      841 cggcggcggg atcctcgctc gcctgatcga agcgccttga gctcacttgt cctcggcacg
      901 cgcgccgccg aggatgccgc gtacgacctc gtagttccct tcatgacacg caaactcgtg
      961 cacgcgatcg ccgtcgtcgc gcgtcagccg cacaaccgcc gtccacggtt tcgtccacgt
     1021 cgtcggatcg tcgacggtga tcgcgtagtc gatcctgtcc gggccgaccc gcgtgaagcg
     1081 ctcggtcagg tgcagccgat ccgccgagcc catgaagtag ctccccggtg agaaattcgt
     1141 cgtttcgacg acgagcgtgt gtccttccca gcgtcctcgc gaatcgccgt tccactgccg
     1201 gatgccgctg ggaagatgcg gacgcccgtc gagcgggatg acgcgagcct cgtggttcac
     1261 ctcgctgagg aacaccacgt agccgggtcc ctggaagatc tggtgatggc tggtgtagtt
     1321 cgtgttgccg ccgatcttgg gcacgcccca ggacatgcag cgcaacatgt tgctcagatc
     1381 ggcggcgccg ttcggaaggc gttgttgcgg cgcaggcgat gccgtgtccg cgccaacctg
     1441 ccacggagtg gtcagcagcg cttgagtgtt ggctcgctgt cgccgcaggc cctccggcgt
     1501 caaggcaggc aggcgtccgt ccgccggatc ggtgatgagg gatgtccggt tgtcgaactc
     1561 ccgcttcacc atgttcagcg aattgccggt cgcattcgga ttgtcaaccg tgtcgaggtt
     1621 cgacag
//
LOCUS       DBNGSH010000309         7539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1892393, whole genome shotgun sequence.
ACCESSION   DBNGSH010000309 DBNGSH010000000
VERSION     DBNGSH010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1892393"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16CY_07265"
     CDS             complement(<1..230)
                     /locus_tag="AC16CY_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353620.1"
                     /translation="MRNLTIGLVSGVFLGAATATLVAQRGAASTYPDAVTADPKHYSV
                     SFENDVARFLRVKYGPGEKSAMHRHLPGCVIFL"
     gene            606..4868
                     /locus_tag="AC16CY_07270"
     CDS             606..4868
                     /locus_tag="AC16CY_07270"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3353621.1"
                     /translation="MRNDVGAEFVLVTSAYAAVVAINRQVPDLVLFSESISDRHRDRV
                     LDHLKSVKGSSGTKTMSIPSLRNGDRSSFAEQIGKSLGKKTLPAKPPTPADTPTARDS
                     SIGASAGLIDPVELELLSQATALETGQHDYDAATDPPEHSVASAEVESLISQIDVGSM
                     AVDIDSDTTPAGEELESEDLYLPIDDLAARAPADRSETASRSVDPEVHAAEIALVQAQ
                     AEAKMAAEVERVRAEEAIQRAAELERIKAEVAAQRDAAAKMAAELERVRKEAAEQRAA
                     ELARIQAEAERQRDAAVKQARAAAQAEARQALSEELSRVRNETERKLADELSRVRESA
                     DQRLAEQLKHTQEAAERAKAEQVAQIQAEAAAMRESEVQEARAAAEQSASRAMAEEVA
                     RVKAETEARLAAELDKVRKDADHQLQAAIERVRDATEHARQENEKRAADEARATAEAA
                     AAHALASEVAKARSEAELQLQAEIERIRMEAAAAARVAQEQSQREAERAREAAAEEAR
                     RAAEHAAARELEAEVAHLRAEADARLKAELEKAREQAERARLKQEEENRKAEARREAA
                     AGAARKAAEDAAARALEAEVARVKAEGDAKLEKELERLRKEAEQARAQSEIETQIVRE
                     TAALEAQEAALAEAARDLEEEMARVRHQADERLASELERLRGEAEAARRSQEEVQREA
                     EKRRDEAAQQARRVAHEAAARALETEVARVREEAEAALKSDLERLRKEATEARLAQAQ
                     TQLETETLRELAIQEARMAAQEAAARELEAEVTRVRAEAEARLNAEVARVRAEAEQAR
                     LNAAANTKGNTETRKAAEESAARALEHELSRLRADADASALAELERLRAEAAEARAAQ
                     EQTQREVEQLRETAAHEARHAAEEAAARALEAEVARVKAEADARLKAEVDRVRQEADA
                     TRRASAESVRTSEAQRDAAAEEARKKAQLEGARALEAEVARLRAEADARLQRELARVN
                     QEAEDARRALEDAQRNAAGLREQEARDLRAAATADAARAFEAEAQRIRAEADARLREE
                     TELAREEANARLEKEVATIKAEAEQRRATELEDVRAQMLRLRTTSAEQARAAAEQAVA
                     KEVARATVVTPPAPAVEPDEREWSEGFVADRALLQRASRVPAPGGKRWVIAAAVALLA
                     VGAVAAFGVFPFGGSKSTEAPAPAAEAPAAPPASTSEPAATADTGELRIETVPTGARV
                     TLDGREVGFTPLTLKAVAVGRHALILQGDSGTVRRTVRVQAGERTVARYEITAGFLSL
                     SSRIPLEIYEGTRKLGTSDGGHLLLAPGSYKVRLVNTRYGFEDVVDVNIRPGEVTTHP
                     VMLPEGSLLVSTEPGSEIFVEGQLAGTAPLGMLQVPIGSREILVRHPRFGERREAVDI
                     RQGKRVELSVVFQDGAQTRPKTQTQPRLPPLSREPDRRPLAR"
     gene            complement(4882..5916)
                     /locus_tag="AC16CY_07275"
     CDS             complement(4882..5916)
                     /locus_tag="AC16CY_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353622.1"
                     /translation="MNCPRTAIAMAGLAFVAFASIASAQPRTIYQQTQYKGQEIGRLT
                     GDVYYARMDDYLSVFMVTPDGIVLVEPIGTEFATWLKGELTRRFAVPVKYVIYSHHHW
                     DHGSGAAVYSDTARIIGHEAMMKRLAMPAASTPLPQNAREQDVNGDGRIERAEARGTI
                     QTQFELYDADRNGALSGAEVARGPLAFVRPPDLTYTSPLTITLGGKRVEVLPRPTAHA
                     DDNTIVRFVDGTGVLFASDWITVKRVPFGGDVAARDELALVKTVEGLDFDHFVCSHGM
                     LGRKADVAANIRYREELRDAVSRAIAAGQTLEQAQASVLMEPYKSWEFYEQQRPANVA
                     GTYRALMGGR"
     gene            complement(5913..6827)
                     /locus_tag="AC16CY_07280"
     CDS             complement(5913..6827)
                     /locus_tag="AC16CY_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase family protein"
                     /protein_id="HIR3353623.1"
                     /translation="MLDLRGERGRWFGGIGASVSSPTLLLSASRASELSVEGEDADRG
                     SEFARRFLRHHGIDTGARIVVHRSLPPHAGLGSGTQLALAVARALADIYGCTSEPREL
                     ARAVGRGRRSAIGTWTFAGGGLVVEGGRPPEGDRSGPLIARLQFPPAWRCVVAVPDGP
                     PGISGADEAAAFARLKKPPERDVERAAHLVLMTLLPALADADIDMFGRALTEIQEITG
                     QWFAEAQGGTFAPGPGRGLVRRFAEWGAAGVGQSSWGPAVYGIVDGADASRHLAERAR
                     EALGSAERVYEGPFCSEGARVWRAKEMP"
     gene            6834..6974
                     /locus_tag="AC16CY_07285"
     CDS             6834..6974
                     /locus_tag="AC16CY_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353624.1"
                     /translation="MQTRMGLDADRALPGGEPGRDQERLLTGVPAAVDCMNARRVSMR
                     GL"
     gene            7121..>7539
                     /locus_tag="AC16CY_07290"
     CDS             7121..>7539
                     /locus_tag="AC16CY_07290"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3353625.1"
                     /translation="MVSTVTRRRFTADEYQRMGQVGILSEGDRVELIDGEIVVMTAMG
                     PRHNASVSAATRALVRAAHDDAIVLPQGSVRLDLYYEPEPDLVLLRPRADFYASRHAG
                     PADILLIIEIADSSLEYDRDVKAPVYAAAGIQEYWLA"
BASE COUNT         1338 a         2598 c         2675 g          928 t
ORIGIN      
        1 agaaagatga cgcagccggg aaggtgccgg tgcatggccg acttctcgcc cgggccgtac
       61 ttcacgcgga gaaagcgggc cacgtcgttc tcgaaggaca ccgagtagtg tttggggtcc
      121 gccgtcacgg cgtccggata agtggaggcc gcgccccgct gcgccaccag cgtggcggtc
      181 gccgccccca gaaaaacgcc ggacacgagg ccgattgtca gatttcgcat acgcgtggct
      241 cccgacccgc ggtcaacgcg ggctcaactc gatggcggcg acatcatcac atagaccgac
      301 ggcgcggcgg caagaggatt gaaccagctt tacaccactg cgctcggcgt ccaaggacgg
      361 atctgaaaaa cggtcgttga ccgaccagag tcgtcagcgc ccggagccgc tgcggccggc
      421 gctctcgctc taagtacgcc tgcaacaacg gagtcggtgg aacttacccg cctcgcctcg
      481 ctccctacag tcgtggcaca tcaattgcga ttctgtcgat ccttaccaag gggtggactc
      541 tgaacgtggg ccgtgtactc gccatcgaac ctgagtcgtc cagggcggaa gctctgcgcc
      601 aaatcgtgcg aaacgatgtc ggcgcggaat tcgtcctggt gacgtccgct tacgcggccg
      661 ttgtcgccat caaccgccag gtgcccgacc tggtgctctt cagtgaatcg atctccgatc
      721 gccaccgcga ccgtgtgctg gatcacctga aatcggtcaa gggctccagc ggcacgaaga
      781 cgatgtcgat cccctcgctc cgcaacggag accgctcgtc gttcgccgag cagatcggca
      841 agtcgctcgg gaagaagacg ttgcccgcca agccgcccac acccgcggat acacccaccg
      901 cgcgcgactc gtcgatcggg gcgtccgccg gcctcatcga tccggtcgaa ctcgagctgc
      961 tgtcgcaggc cacggcgctc gaaaccggac agcacgacta cgacgccgcg accgatccgc
     1021 cagaacactc ggtcgccagc gcggaggtcg agtcgctcat cagccagatc gacgtggggt
     1081 cgatggcggt ggacatcgac agtgacacca cgccggccgg tgaggaactg gaatcggagg
     1141 atctgtacct acccatcgac gatctggcgg cacgcgcacc ggcggaccga tcggaaacgg
     1201 cgagccgctc ggtcgatccg gaagtgcacg ccgcggagat cgcgctggtc caggcccagg
     1261 cggaagcgaa gatggccgcc gaggtcgagc gcgtgcgcgc ggaggaggcc atccagcgcg
     1321 cggccgaact ggagcgcatc aaggccgagg tcgcggcgca gcgggacgcc gccgcgaaga
     1381 tggccgcgga gctcgaacgc gtgcggaagg aggcggccga gcaacgggcc gccgagctcg
     1441 cgcgcattca ggccgaggcg gagcgtcagc gggacgccgc cgtgaagcag gccagggcgg
     1501 cggcccaggc cgaggcccgt caggcgctga gcgaagaact gtcccgggtg cgcaacgaaa
     1561 ccgagcgcaa gctggcggac gaactctcgc gcgtacgcga gagcgccgat caacggctcg
     1621 ccgaacagtt gaagcacacg caggaggccg ccgagcgcgc caaggccgag caggtcgcgc
     1681 aaatccaggc cgaagccgcg gcgatgcgcg aaagcgaggt gcaggaggcc agggccgccg
     1741 cggagcagtc ggcctcgcgc gcgatggccg aggaagtggc gcgcgtgaaa gcggagaccg
     1801 aggcgcggct ggccgcggaa ctggacaagg tgcgcaagga cgcggaccac cagctgcagg
     1861 ccgcgatcga gcgcgttcgc gatgcgaccg agcacgcgcg gcaggaaaac gagaagcgcg
     1921 ccgccgacga ggcgcgtgcg accgccgagg cggccgcagc gcacgcactc gcgtccgagg
     1981 ttgcgaaagc gcgatccgag gcagagctcc agctacaggc cgagatcgag cgcatccgca
     2041 tggaggccgc cgccgccgcg cgcgtcgcac aggagcagtc acagcgcgag gcagagcgcg
     2101 cccgtgaagc agctgccgaa gaggcgcggc gggccgcgga acacgcggcg gcgcgggagc
     2161 tcgaggcgga ggtcgcccac ctgcgggcgg aagccgatgc gcgtttgaag gccgagctcg
     2221 agaaggcacg cgagcaagca gaacgcgcac ggctgaaaca ggaagaggag aaccgaaagg
     2281 cggaggcgcg ccgcgaggcg gccgcgggcg ccgcgcgcaa ggccgcggaa gacgcggcgg
     2341 ccagggcgct cgaggccgaa gtcgcgcgcg tgaaggccga aggggacgcc aagctcgaga
     2401 aggagctcga acgcctgcgg aaggaagcgg agcaggcgcg cgcgcagagc gaaatcgaga
     2461 cccagattgt tcgcgagacc gccgcgctcg aagcgcagga ggcggcgctc gccgaagccg
     2521 cgcgcgacct cgaggaggaa atggcccggg ttcgtcacca ggcggacgag cggctggcca
     2581 gcgagctgga gcggctgcgc ggagaagccg aggccgcacg ccggtcgcag gaagaggtgc
     2641 agcgcgaggc cgagaagcgc cgcgacgaag ccgcgcagca ggcgcggcgt gtggcgcacg
     2701 aagccgcagc gcgggcgctc gagaccgaag tggctcgcgt ccgcgaggaa gccgaggccg
     2761 ccttgaagtc agacctcgag cggctccgca aggaggccac cgaggccagg ctcgcccagg
     2821 cgcagacgca gctcgagacc gaaacgctgc gcgagctggc gattcaggaa gcgcggatgg
     2881 ccgcgcagga ggcggccgcc cgcgagctcg aagcggaagt gacccgagtc agggccgagg
     2941 ccgaggccag gctcaacgcc gaggtcgcgc gcgtgcgggc cgaggccgaa caggctcgac
     3001 tgaacgcggc cgccaacacc aagggcaaca ctgaaacgcg caaggccgcc gaggaatcgg
     3061 cggcgcgggc gctcgaacac gaactctccc gcctgcgcgc ggacgccgac gccagcgcgc
     3121 tcgcggagct cgaacgcctg cgtgccgagg cagccgaggc ccgggccgcg caggagcaga
     3181 cacagcgcga ggtcgagcaa ctccgcgaga cggccgcgca cgaggcacgg cacgccgccg
     3241 aggaagccgc ggcacgcgcc ctcgaggcgg aggtcgcgcg agtcaaggcc gaagccgacg
     3301 cgcggctgaa ggccgaagtc gatcgcgttc gtcaggaagc cgacgcgacg agacgggcga
     3361 gcgcagagtc ggtgcgcacc agcgaggcgc agcgcgatgc cgcggcagag gaagcccgca
     3421 agaaggcgca gctggaaggg gcgcgcgcgc tcgaagccga ggtggcgcgc ctgcgcgccg
     3481 aagcagacgc ccggctccag agggagctcg cacgcgtcaa tcaggaagcc gaggatgcgc
     3541 ggcgagccct cgaagacgca cagcgtaacg ctgccgggct tcgcgagcag gaggcccggg
     3601 accttcgggc tgccgcgacc gcggacgcgg cgcgggcctt cgaggccgag gcgcagcgca
     3661 tccgggccga ggccgatgcc cggcttcgcg aagagacgga gctcgcgcgt gaggaggcca
     3721 acgccaggct cgagaaggaa gtggccacga tcaaggccga ggccgaacag cggagggcca
     3781 ccgagctcga ggacgtccgc gcgcagatgc ttcgcctgcg taccacgtcg gccgaacagg
     3841 cgcgcgccgc tgcggaacag gccgtggcca aggaggtcgc acgcgccacg gtcgtcacgc
     3901 ccccggcgcc cgccgtcgag ccggacgagc gcgagtggtc cgaggggttc gtcgccgatc
     3961 gggcgctgct gcaacgagct tctcgcgtgc cggccccggg cggaaaacgc tgggtcatcg
     4021 ccgccgccgt cgcgctgctg gccgtgggcg cggtggccgc gttcggtgtg ttccccttcg
     4081 gaggatcgaa atccacggag gccccggcgc cggcggccga agcccccgcg gccccacccg
     4141 ccagcaccag cgagccggcc gcaacggccg ataccggcga gctcaggatc gagacggtgc
     4201 ccacgggcgc tcgcgtcacg ctcgacggac gcgaggtcgg cttcaccccg ctgacgctga
     4261 aggccgtcgc cgtcggtcgg catgccctca tcctgcaggg ggattcgggc actgtgcgcc
     4321 gcacggtgcg ggtgcaggcc ggcgaacgca cggtcgcccg ctacgagatc accgccggct
     4381 tcctgtcgct ctcgtcgaga attccgctcg agatctacga agggacccgc aagctcggca
     4441 cgagcgacgg aggacatctg ctgctcgcac caggcagcta caaggtcagg ctcgtcaaca
     4501 cgcgctacgg cttcgaggac gtcgtcgacg tgaacatcag gccgggcgag gtcacgacgc
     4561 accctgtcat gctgccggaa ggatcgctgc tcgtgagcac cgagccgggc tcggagatct
     4621 tcgtcgaagg ccagctcgcg gggacggcgc cgctcggcat gcttcaggtc cccatcggat
     4681 cgcgggagat cctcgtccgg catccgcgct ttggcgagcg ccgcgaggcg gtggacatca
     4741 ggcagggcaa gcgcgtggaa ttgagcgtcg tgttccagga tggcgcgcag acgcggccga
     4801 agacccagac ccagccgcgc ctgccgccgc tcagcaggga gccggaccgc cggccgctcg
     4861 cgcgctgaga tctccgacag cctatcttcc gcccatcagc gcccggtagg tcccggccac
     4921 gttcgcgggg cgctgctgct cgtagaactc ccagctcttg tagggctcca tcagcacgct
     4981 cgcctgggct tgttcgagcg tctgccccgc ggcaatcgcc ctggacaccg cgtcgcgcag
     5041 ctcctcgcgg tagcgaatat tggccgcgac atcggccttc cggccaagca ttccgtgact
     5101 gcagacgaag tggtcgaagt cgagaccctc gaccgtcttg acgagcgcca gttcgtcccg
     5161 ggcggccacg tcgccgccga acggcacgcg cttcaccgtt atccagtcgg aagcgaacag
     5221 cacccccgtg ccgtccacga aacggacgat ggtgttgtca tcggcgtgcg ccgtgggccg
     5281 cgggagcacc tccacgcgtt tccccccgag cgtgatcgtc aacgggctcg tgtacgtcag
     5341 gtccggtgga cgaacgaacg ccagcgggcc ccgggcgacc tcggcgccgc tcagggcgcc
     5401 gttgcgatcg gcgtcgtaga gctcgaactg tgtctgaatg gtgccccgtg cctcggcgcg
     5461 ctcgatcctg ccgtcgccgt tgacgtcctg ctcgcgcgcg ttctgcggaa gcggcgtgct
     5521 cgcggcaggc atggccagac gtttcatcat cgcttcgtgc ccgatgatgc gcgcggtgtc
     5581 ggagtacacc gccgccccgg acccgtggtc ccagtgatgg tgactgtaga tgacgtactt
     5641 caccggaacc gcgaatctcc tggtcaactc ccctttcagc cacgtggcga attcggtccc
     5701 aatcggttcg acgaggacga ttccgtccgg cgtgaccatg aagaccgaca ggtagtcgtc
     5761 catgcgcgcg taatagacat cgccggtgag cctgccgatc tcctggcctt tgtactgcgt
     5821 ctgctgatag atcgtccggg gctgcgcgga ggcgatggat gcaaacgcga cgaaggcgag
     5881 gcccgccatc gcgatcgcgg tacgtggaca attcatggca tctccttcgc gcgccacaca
     5941 cgcgcgccct cagagcagaa cggaccctcg tagactcgtt cggcggagcc gagcgcctcc
     6001 cgtgcacgtt cggccaggtg ccggctcgcg tcagcgccat cgacgattcc gtagacggcg
     6061 gggccccacg agctctggcc cacgccggcc gcgccccact cggcaaagcg gcgtaccagg
     6121 ccgcggcccg ggccgggggc aaacgtcccg ccctgcgcct cggcgaacca ctgccccgtg
     6181 atctcctgaa tctcggtcag cgcgcggccg aacatgtcga tgtctgcgtc ggccagcgcg
     6241 ggcagcagcg tcatcaggac gagatgcgcc gcccgttcca cgtcccgttc gggcggcttc
     6301 ttcaaccgcg cgaacgcggc cgcctcgtca gcgccgctga tgcccggcgg gccatcaggc
     6361 acggcgacga cacatcgcca cgccggcgga aactgcagac gggcgatcag cggaccgctc
     6421 ctgtcgccct ccgggggccg cccaccctcg acgaccaggc caccgcccgc gaaggtccac
     6481 gttccgatcg ccgatcgacg accgcggccc acggctcgcg ccagctcgcg cggctcgctc
     6541 gtgcagccgt agatgtcagc cagcgcgcga gccacggcca gggccagctg cgtaccggag
     6601 ccgagaccgg cgtgcggggg cagcgaccgg tgcaccacga tccgggcgcc cgtgtcgatg
     6661 ccgtgatgac gcaggaatcg ccgcgcgaac tcgctgccgc gatcggcatc ctcgccttcc
     6721 acgctgagct cgctggcacg cgacgcagac agcagaagcg tcggcgacga caccgaggcc
     6781 ccgatgcctc cgaaccagcg cccgcgctcg ccccgcagat cgagcacgcc gaagtgcaga
     6841 cgcgcatggg cctcgacgca gaccgcgccc tgcccggcgg cgagcccggc cgggatcagg
     6901 agcggctcct caccggcgtg ccagcggcgg tcgactgcat gaacgcgcgt cgcgtcagca
     6961 tgcgcggatt atagccaagg cgtgtgggcg tctgaattct ccacatcgga cgcgcgatcg
     7021 ccgctgcagg aactgcaagg tctgggacat tcacgcgcgt tcgccgcgac attccgcgcg
     7081 gcacgaccct cgcatcaacg gctagaatgg agatgtctct atggtcagta ccgtcaccag
     7141 gcggcgcttt accgccgacg aataccagcg catgggacag gtcggtatcc tgtccgaagg
     7201 cgatcgcgtg gagctcatcg acggtgagat cgtcgtgatg acggcgatgg gccccaggca
     7261 caatgcgagt gtgagcgctg ccactcgcgc attggtcaga gcggcgcacg acgacgcgat
     7321 cgtcctgcct cagggctcgg tgcggctgga cctgtattac gagcctgagc cagacctggt
     7381 cctgctgcgg cctcgcgccg acttctacgc ttcgcgtcac gcaggcccgg ccgatatcct
     7441 gctcatcatc gagattgccg actcctcgct cgagtacgat cgcgacgtga aagcgccggt
     7501 ctatgcggcc gccggcattc aggagtactg gctcgccga
//
LOCUS       DBNGSH010000310         4918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_704373, whole genome shotgun sequence.
ACCESSION   DBNGSH010000310 DBNGSH010000000
VERSION     DBNGSH010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_704373"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="AC16CY_07295"
     CDS             complement(<1..232)
                     /locus_tag="AC16CY_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353626.1"
                     /translation="MKKPNLRVIALCLLLTPAGPALASQSSQKLGASPAPATAPASVP
                     PGYVIGVDDALSVRFWGPDEKLSGDVVVRPDGK"
     gene            complement(501..1868)
                     /locus_tag="AC16CY_07300"
     CDS             complement(501..1868)
                     /locus_tag="AC16CY_07300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR3353627.1"
                     /translation="MTISARNTSTGAATTSFEQLSELIHTKQALVGVIGLGYVGLPLV
                     RAFTAAGFRCLGFDVDQSKVDKLNAGESFIKHIDAAAFLGMIREKRFEPTADMRRLSE
                     ADCIIICVPTPLNDSRDPDLSYIDGTARSIARALRPGQLVVLESTTHPTTTRTNVLPV
                     LEATGLRCGEDYFLAFSPEREDPGNPHFEAASIPKVVGGYDERSAELASAMYGPAVVQ
                     VIRVSSLEVAEASKILENTYRAVNIALVNELKVLYDKLGIDVWEVIEAAKTKPFGFQA
                     FYPGPGLGGHCIPIDPFYLTWLARKHGEQTRFIELAGEVNAHMPQYVVNRLGEFLNEQ
                     RKPINGSRIAILGVAYKKDVDDARESPAFELMRLLFSRGAEISYNDPHIPVLPRVRHY
                     PNLPEMRSAELTRDFLGAQDCVLVATDHSAYDYEFIVEHSKMVLDTRNATRLVTRGRE
                     KIRKA"
     gene            complement(1880..2638)
                     /locus_tag="AC16CY_07305"
     CDS             complement(1880..2638)
                     /locus_tag="AC16CY_07305"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR3353628.1"
                     /translation="MSVRQSGTISPGEAHVWWVDPEALREEVTSRLFVLSAEERARMA
                     SFRFESDRAAYAAAHLLLRSALTWCAPGIPPATWELTSDRLERPEVVAPNTCPKLRFN
                     ISHTRSLVACLVTREIDCGVDVEVRRQVEDMEALAARVLSPAELSGLMALPESLRPTR
                     FFSYWTLKEAYVKARGLGLSLPLDAVSFDLGVEGIGITIDPSLNDHGRDWQFEQWAPT
                     EHHILAVAIKSGPSPGARVVRHPECPVGQLRDVC"
     gene            complement(2659..4155)
                     /locus_tag="AC16CY_07310"
     CDS             complement(2659..4155)
                     /locus_tag="AC16CY_07310"
                     /inference="COORDINATES: protein motif:HMM:NF028171.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3353629.1"
                     /translation="MTVGLSSASARTCVALGDEIGALMVLEQERATRIRGAGVESSGL
                     PDEALDLLLDLKCSSRADIGRYVRVGAGWGADVQSRDRMEPHFALACTAYLSSPYSAA
                     TIVVCDREEPKVSVWRARGREVARVDWPWVGPGYADLLSECSRLVGFSSSAGAQRFEA
                     LARLEPGNGDERAAAFIDGDAHSVTAKPGWELAVGSRIAQETARGGIVACAAIAAGLQ
                     RRLAELFLALLAEVQRHTQDEHVCLAGSLFYNSSFNTAAHLAGPFTDVFVPVDPGLTG
                     AAVGAALASIGRPPQLLSPFLGPWYDQAAIKATLENCKLQYDWQSDDAVIDAAVSALC
                     RGRLVAWFEGPMEWGHRALGARSIVANPFAPYVLENLNGFLKKRQPWRGYALSSLHGA
                     LREHFDGPDKAPFMECDFRPRDPDRFRHVLPARSALVRVHSVDHRSPPRFRQLLTAFG
                     EATGIPCLVNTSFNGFHEPIVCNARDAVRVFYGTGVDVLALENFVVVK"
     gene            complement(4239..>4918)
                     /locus_tag="AC16CY_07315"
     CDS             complement(4239..>4918)
                     /locus_tag="AC16CY_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3353630.1"
                     /translation="PGRRVYRTGDIGRRLPDGTLEFVGRKDTQVKIRGFRIELGEVES
                     VLGGHPGVQECAVVARAEADGSVQLVAYVRPSTSQGDVATELRNFLKQKLPTYMVPAT
                     FVVMDALPFTQNGKIDRQALPAPAKTKTWGGGARVWDAANVSSRPSTDAEIAIARIWK
                     SVIGTDTVSVDDKFFDLGGHSLNALQVISIIKRELGCRITFNDLMYQTLRQAAAACEK
                     QLAEQAQ"
BASE COUNT          893 a         1622 c         1525 g          878 t
ORIGIN      
        1 tcttgccgtc gggccgcacg accacatcgc cagagagttt ttcgtctggc ccccagaaac
       61 gaacgctgag cgcatcgtcc acgccgatca catagccggg tggcacactc gccggtgcag
      121 tcgccggcgc tggtgacgcc cccagcttct gcgacgactg cgacgccagc gcggggcctg
      181 caggggtcag cagcagacac agggcgatta ccctcaaatt tggtttcttc attcggcaac
      241 ctcgaattcc ctgaggtgtc aataaaccga cactccgtgt gcgtgccttt ggcgccgaaa
      301 cgcgcaattg cagtgtgcaa gggacccgcc agtcatgccg tcaacggatg ggcccgcttg
      361 tccagtttgg acgccgactt cacggcgcca gattggaatc gccggcagcc agggcggcca
      421 ccgatcctag ccgattggat tcttcagaaa gaagtccgcc tataagcaga acacctgcaa
      481 acgccgggcc ccgcggatcc ctaggctttg cggatttttt ctctccctct ggtcacgagc
      541 ctggttgcgt tgcgcgtgtc gagcaccatc ttggagtgct ccacaatgaa ctcgtagtcg
      601 tacgcggaat ggtcggtggc gaccaatacg caatcctggg caccgaggaa gtcgcgcgtc
      661 agctctgcgc tccgcatctc cggcagattc ggataatgcc ggacccgcgg cagcactgga
      721 atgtgaggat cgttgtagct gatttcggcg ccgcggctga aaagcagcct catgagctcg
      781 aacgccgggc tctcgcgggc gtcgtcgacg tccttcttgt acgccactcc gagaatcgca
      841 atccggctgc cgttgattgg cttccgctgc tcgttcagga actcgcccag gcgattgacg
      901 acgtactgag gcatgtgcgc gttgacttcg cccgccagtt cgatgaaacg ggtctgttcc
      961 ccgtgcttgc gcgcgagcca cgtgaggtag aacgggtcaa tcgggatgca gtgccccccg
     1021 aggcccggtc cgggatagaa ggcctggaag ccgaacggct tggtcttcgc ggcctcgatc
     1081 acctcccaca cgtcgatgcc gagcttgtcg tagagaacct tcagctcgtt gacgagtgcg
     1141 atgttcaccg cccgatacgt gttctcgagg atcttgctcg cctcggccac ttcgaggctc
     1201 gacacgcgta tgacctgaac gacggcgggc ccgtacatcg ccgaggccag ctccgcgctt
     1261 cgctcgtcgt agcctccgac gaccttcggg atgctcgccg cttcgaaatg cgggttgccg
     1321 gggtcttcgc gctccgggct gaacgccagg aagtagtcct ctccacagcg cagccccgtg
     1381 gcctcgagca ctggcagtac gttcgtacgg gtggtcgtcg ggtgcgtcgt gctctcgaga
     1441 acgacgagct gcccgggacg cagggcccgc gcgatcgaac gcgccgtccc atcgatgtag
     1501 ctcaagtccg ggtcacggct gtcgttgagc ggcgtgggca cgcagatgat gatgcagtcg
     1561 gcctccgaga gcctcctcat atccgcggta ggctcgaatc gcttctcccg aatcatcccg
     1621 agaaaggccg ccgcatcgat atgtttgatg aagctttctc cggcgttcaa cttgtccact
     1681 ttcgattgat cgacatcgaa ccccaggcag cggaaaccgg cggcggtgaa cgcccgaacc
     1741 agtggtaagc cgacatagcc gagcccgatc accccgacca gggcttgctt cgtgtgaatc
     1801 aactcggaga gctgctcgaa gctggtcgtc gctgctcctg tagacgtgtt ccgtgccgat
     1861 atagtcattc ctgaacctct caacaaacgt ccctcaattg tcccaccgga cactccggat
     1921 gtcgcacgac gcgggcgccg ggcgacgggc cgcttttgat ggctactgcc agaatgtgat
     1981 gttcggttgg cgcccactgc tcgaactgcc agtctcgacc gtggtcgttc aatgacgggt
     2041 caattgtgat tccgatgcct tcgacgccga ggtcgaaaga cacggcatcc aacggcaacg
     2101 acaaccccag gcctcgtgcc ttgacatacg cctccttcaa ggtccagtaa ctgaagaacc
     2161 gggtcggccg tagggattca ggcagcgcca tcaagcccga cagctccgca ggcgacagga
     2221 ctcttgcggc aagcgcctcc atgtcttcta cctgccgccg gacctccacg tccacaccgc
     2281 agtcgatttc gcgagtgacg agacacgcaa caagactgcg ggtatgcgag atattgaacc
     2341 gcagtttggg gcacgtattc ggggcgacca cttccgggcg ctcgagtcga tcgctcgtca
     2401 gttcccacgt ggccggcggg atgccggggg cacaccacgt gagggctgaa cgaagcagaa
     2461 ggtgtgcggc ggcgtaggcg gctctgtccg actcaaagcg gaagctcgcc atccgtgcgc
     2521 gctcctccgc cgagagaacg aacaggcggc tcgtcacctc ttcgcggagc gcctccggat
     2581 ccacccacca gacatgagct tcgccagggg aaatcgtgcc agattggcga acgctcacag
     2641 tcgtcccggg agcgggcatc acttgacgac gacaaagttt tcgagcgcga ggacgtcgac
     2701 accggttccg tagaacaccc gcacggcgtc gcgggcgttg cagacgatcg gctcgtgaaa
     2761 tccgttgaac gacgtgttga cgaggcacgg aattccggtc gcctcgccaa acgccgtcag
     2821 gagctgtcgg aatcgaggag gcgacctgtg atcgacgctg tgcaccctca ccagtgcgct
     2881 gcgggccggc aagacgtgcc ggaagcgatc cggatcgcgc gggcggaaat cgcattccat
     2941 gaacggggct ttgtcgggcc cgtcgaagtg ctcgcgcagg gccccgtgca gggagctgag
     3001 tgcatatcct cgccacggtt gccgcttctt caagaaacca ttcaagttct cgagcacgta
     3061 cggcgcgaag gggttcgcga cgatcgatct ggcacccagc gcgcggtgcc cccattccat
     3121 cgggccctcg aaccacgcga cgagtcgccc cctgcacaag gccgatacag ccgcgtcgat
     3181 aacagcgtcg tcgctctgcc agtcgtactg cagtttgcag ttctcgagcg tagccttgat
     3241 cgcggcctga tcgtaccagg gaccgaggaa cggcgagagc agctgaggcg gacgaccgat
     3301 cgaggcgagg gccgcgccga ccgccgcgcc ggtaagtccc ggatcgacgg gcacgaagac
     3361 gtccgtgaac ggtccggcga ggtgcgccgc ggtgttgaac gaagaattgt agaagaggct
     3421 gccggccagg cagacgtgtt cgtcctgcgt gtgtcgctgg acctctgcga gcaacgcgag
     3481 gaacagttcg gccagccgcc gctgcagccc agcggcgatg gcggcgcacg ccacgattcc
     3541 cccacgcgcc gtctcctgcg cgatacgcga accaacggcc agctcccagc ccggtttcgc
     3601 ggtcacggag tgcgcatccc catcgatgaa cgcggcggcg cgctcgtcgc cgtttccagg
     3661 ctccagtctg gccagagcct cgaagcgctg tgccccggct gacgaggaga agcccacgag
     3721 acggctgcac tcggagagca ggtcggcgta gcctgggcca acccagggcc agtcgactct
     3781 tgccacttct ctgccgcgcg cccgccagac gctgaccttg ggctcctcgc ggtcgcacac
     3841 gacgatcgtc gcggccgaat agggcgagga caggtaggcc gtgcaggcga gagcgaagtg
     3901 gggctccatc cgatcgcggg actgaacgtc cgctccccac ccggcgccga cgcgcacgta
     3961 gcggccgata tcggcgcgac tcgaacattt gagatccagg agcagatcga gggcctcgtc
     4021 cggcaggccg ctcgattcga cgcccgcgcc acgaatgcgg gtcgcgcgct cctgctccag
     4081 aaccattagg gcgccgatct cgtccccgag cgcaacgcag gtacgcgccg acgcgctcga
     4141 gagccccacc gtcattcgtc cattcacgcc agtcgaacgc atgggcagat ctcgaccctc
     4201 acgagcgctc ttctcgcggc gatcatgcgc aggtgacact actgagcctg ttccgccagc
     4261 tgcttctcgc atgccgctgc agcctgtcgc agagtctgat acatcaggtc gttgaacgtg
     4321 atcctgcacc ccaactcccg cttgatgatc gagatgactt ggagggcgtt cagcgagtga
     4381 ccgccgagat cgaagaactt gtcgtcaaca gacacggtgt cagtgcctat gacgctcttc
     4441 cagattcggg cgatggcgat ttcagcgtcg gtgctcggcc gcgaggacac gttggcggcg
     4501 tcccagacgc gcgccccgcc gccccatgtc ttcgtcttcg ccggcgccgg taaggcctgc
     4561 cggtcgatct tgccgttctg cgtgaacggc agggcatcca tcaccacgaa cgtcgccggc
     4621 accatgtagg tgggcagctt ctgtttcaag aagttcctca gctcggtggc gacgtccccc
     4681 tgggacgtcg aaggccgaac gtacgcgacg agctgaacgg atccgtcggc ttccgcgcgt
     4741 gccacgactg cacattcctg taccccggga tgtccgccga gcactgactc gacctcgccg
     4801 agctcgatcc ggaagccacg gatctttacc tgcgtgtcct tgcggccaac gaactccagc
     4861 gtgccgtccg gaagccggcg accgatgtcc cccgtccgat agacccggcg tccggggt
//
LOCUS       DBNGSH010000311         4322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4269860, whole genome shotgun sequence.
ACCESSION   DBNGSH010000311 DBNGSH010000000
VERSION     DBNGSH010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4269860"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="AC16CY_07320"
     CDS             <1..332
                     /locus_tag="AC16CY_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353631.1"
                     /translation="TGLRLDDHEDVDLPGKNVYRVDELMRLEQNLEIQTEIISRAHAF
                     VGTYGGLAYLGPFYGVPSISFYSSEEELVPAHLDVSWRLGRTMGVPVTTLHTKMAGLL
                     RGMLSER"
     gene            335..1516
                     /gene="cax"
                     /locus_tag="AC16CY_07325"
     CDS             335..1516
                     /gene="cax"
                     /locus_tag="AC16CY_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015369 - calcium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HIR3353632.1"
                     /translation="MRLLLAEIRHNPLLWLLAFVPVVFAGARLWADAHTLLFLLSVLA
                     IVPLAALLSHATESVASRTGDAVGGLLNATLGNLTELVIALAALRAGEYTLVKASIAG
                     AIVANTLFMLGMSFLLGGLKYHIQEFNRVNARLQAGLLFLATVALLVPSVVSQVDEAP
                     AFAQRLSVGLSVLLIAAYGLGLLFSLKTHREIFGSVGHGEGDEAPWPLGLGLATLAGV
                     TILVALVSEIFVESVQGAALELGMTPAFVGFIVVALVGAAAEMASAFSGARKNRLDLS
                     VGIALGSAAQIALFVAPALVLISYVLGPTPMSLQFWPGAVVMMFIATMTASLVTNSGR
                     SAWFVGVLVLMVYLIFAMTLYLLPPRGDTEEARSQAASFFSSALSESIFPAPITTAES
                     G"
     gene            complement(1438..2451)
                     /gene="mreB"
                     /locus_tag="AC16CY_07330"
     CDS             complement(1438..2451)
                     /gene="mreB"
                     /locus_tag="AC16CY_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR3353633.1"
                     /translation="MAFAGLGSDLAIDLGTANTCVFALGRGVVVSEPSLIAFHTTTGA
                     IEAVGDDAREMLGRTPGHLRPVRPIRDGVIADFDAAEKMLNHFIRKTCRHVGGWARPR
                     VVIGVPAEITPVERRAVKDSAYRAKASEVFLVDEPMAAAVGAGLPIGEPAGNMIIDVG
                     GGTTDIAVISLAGVVIGRSVRVAGDAFDTAITTHLKKKHEVLIGERTAEEIKLQIGSA
                     TALEKPLSMEVKGRHFARGVPVRVKVSDAEIREAISEPLTTILKAIRETLDQIPPELS
                     ADIFDRGIALCGGGALLRNLDRRINQETQLPVKIVDQPLSAVVIGAGKMLSDKALLKK
                     LAA"
     gene            2623..3354
                     /locus_tag="AC16CY_07335"
     CDS             2623..3354
                     /locus_tag="AC16CY_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3353634.1"
                     /translation="MSRLSTLFLVIAASSIVLESSQSPGGRAARGIWDGVFTRAQAER
                     GRLSYREHCATCHGADLQGAEYKALQGNRFWTDYQEAPLDYLLGQISRNMPHSEDGSL
                     KGTLGASTYADIVAFMLDANGFPAGATELTEASSAGVAIVRKEGPGELPVGSFAHVVG
                     CLAPRGPDNNWRLVKGSRPVRVLGNRPVDAKAALGNREYTLMYVLTSLDKYAGYRMSV
                     RASLMEGGNGALNVQSIQAVSPTCE"
     gene            complement(3409..4035)
                     /locus_tag="AC16CY_07340"
     CDS             complement(3409..4035)
                     /locus_tag="AC16CY_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3353635.1"
                     /translation="MLVSLVVLAASAPASAQDFPRVDVSGGYQGVIADEEFFTTGWYF
                     DVAGNVTSLIGIVFQVGGNSKSFSETNTFTDPGFPPFVPATTITDIVDEDFSLNQYMG
                     GVRLNARGGRVTPFGQFLVGVVKSSSEGTFTSTITFTPPSPAFPPIFESESFSDTSTD
                     FAIQVGGGVDIRIAGGFGIRAGGDYLRVSGEEEAVNMFRFAVGALYSF"
BASE COUNT          745 a         1399 c         1392 g          786 t
ORIGIN      
        1 acaccgggct caggctcgac gaccacgagg acgtggacct gcccgggaag aacgtgtacc
       61 gcgtagacga gctgatgcgg ctcgagcaga acctggagat ccagaccgag atcatcagcc
      121 gcgcccatgc gtttgtcggg acttacggag ggctggcata cctcggcccg ttctacggcg
      181 tgccttccat ctcgttctat tcgtctgaag aggagctcgt cccagcccac cttgacgtca
      241 gctggcggct cggacgcacg atgggcgtcc cggtgacgac gctgcacacg aagatggccg
      301 gcctgttgcg cggtatgctg agcgagcgtt agccatgagg cttctcctcg cggaaatccg
      361 ccataacccg ctgctctggc tgctggcctt cgtgcctgtg gtgttcgccg gcgcgcggtt
      421 atgggcggac gcgcacacgc tgctgttcct gctgtcggtg ctcgcgatcg tgccgctcgc
      481 cgcgctgctc agccacgcca ccgagtcggt cgcctcgcgc acgggcgatg ccgtcggcgg
      541 cctcctcaac gccacgctcg gaaacctgac ggagctcgtc atcgcccttg ccgcgctgcg
      601 cgccggggag tacaccctcg tcaaggcctc catcgccggc gccatcgtcg ccaacaccct
      661 cttcatgctc ggcatgtcgt tcctgctcgg cgggctcaag tatcacatcc aggaattcaa
      721 cagggtcaac gcccggctcc aggccggcct gctgtttctc gcgaccgtcg ccctgctcgt
      781 tccctcggtc gtcagtcaag tcgacgaggc gcccgccttt gcgcaacgcc tgagcgtcgg
      841 gctctctgtg ctcctcattg ccgcttatgg cttgggcctg ctgttctcgt tgaagacgca
      901 tcgggagatc ttcggaagcg tcgggcacgg cgaaggcgac gaggccccgt ggccgctcgg
      961 gctgggactg gctacgttgg ccggggtcac catcctcgtt gcgctggtga gcgagatctt
     1021 cgtcgagtcg gtgcagggcg cggcgctcga actggggatg acgcccgcct tcgtgggctt
     1081 catcgtggtg gcgctggttg gcgctgcagc ggaaatggcc tcggcgtttt caggtgcgcg
     1141 caagaaccgg ttggatctga gtgtaggcat cgctctcgga agcgcagccc agattgcgct
     1201 cttcgtggcg ccggcactcg tcctgatcag ttatgtcctc ggtccgaccc ctatgagcct
     1261 gcagttctgg cccggcgcgg tggtgatgat gttcatcgcg acgatgacgg cgtcgctcgt
     1321 gaccaacagc ggccgctctg catggttcgt cggcgtgctg gtgctgatgg tctatctgat
     1381 cttcgcgatg acgctctacc tgctgccacc gcgcggcgac acggaagaag ctcggtctca
     1441 ggcggccagc tttttcagca gcgccttgtc ggagagcatc ttcccggcgc cgatcacgac
     1501 ggcggagagc ggctgatcga cgatcttgac cggcagctgc gtctcctggt tgatgcggcg
     1561 atcgagattg cggagaagcg ccccgccgcc gcacagtgcg atcccccgat cgaagatgtc
     1621 cgccgagagc tccggtggaa tctgatccag cgtttcccgg atggccttca ggatcgtggt
     1681 cagcggctcg gagatcgctt cgcggatctc tgcgtcggac actttcaccc gaaccgggac
     1741 gcccctggcg aaatggcgcc ccttgacttc catcgaaagc ggcttctcga gcgccgtggc
     1801 cgagccgatc tgcagcttga tctcctcggc cgtccgctcg ccgatgagga cctcgtgctt
     1861 cttcttcagg tgcgtggtga tcgccgtatc gaacgcgtcg cccgccacgc gcaccgagcg
     1921 gccgatgacg acgcctgcca gcgagatcac ggcgatatcg gtggttccgc cgccgacatc
     1981 gatgatcatg ttgcccgcgg gttcgccgat cggcagcccc gcgccgacgg cggctgccat
     2041 cggctcgtcc accaggaaca cttcgctcgc cttggcgcgg tacgcgctgt ccttcaccgc
     2101 gcgccgctcg acgggagtga tctccgccgg cacgccgatg acgacccggg gacgagccca
     2161 gcccccgacg tgtcggcacg tcttgcggat gaaatggttc agcatctttt cggcggcgtc
     2221 gaagtcggcg atcacgccgt cccggattgg ccggacaggg cgcaggtgcc ctggtgtccg
     2281 ccccagcatc tccctcgcgt cgtcgccgac ggcctcgatc gcgcccgtgg tcgtgtgaaa
     2341 cgcgatcagg gatggctcgc tgacgaccac tccgcggccg agcgcgaaga cacaggtgtt
     2401 ggccgtgccg aggtcgatgg cgaggtcgga gccgagaccg gcaaaagcca tgatggagac
     2461 aagtccaggc gcaaagtccg ttccgcaacg agcctggatt ccacgatcga aaactgttcg
     2521 gaaatctgga gcaccgggtg gtcgcacgtc tgagcaggcg ccgaatcgga ttacggaaag
     2581 gggcgcgcgc gtgacactaa tgtgacgcta tcatgctgat ccatgtcgcg cctctctacc
     2641 ctgttcttgg tcatcgccgc gtcttcaatc gtcctcgaaa gctcgcagtc gccgggcggc
     2701 cgtgcggcca gaggcatctg ggatggtgtc ttcaccaggg cgcaggccga gcgcgggcgg
     2761 ctctcgtatc gcgagcactg cgccacctgc cacggcgccg atcttcaggg ggccgagtac
     2821 aaggcactcc agggaaaccg cttctggacc gactaccagg aggcccctct cgactacctg
     2881 ctcggccaga tcagccgaaa catgccgcat tccgaggacg gctcgctcaa gggcaccctg
     2941 ggcgcgagca cgtacgccga catcgtcgcg ttcatgctgg acgcgaacgg attcccggcc
     3001 ggcgccactg agttgaccga ggcgtcgagc gccggcgtgg cgatcgtcag gaaggaaggc
     3061 ccgggcgagc tgcccgttgg cagcttcgcc cacgtggtcg gatgtctcgc tccgcgcggc
     3121 ccggacaaca actggaggct cgtcaagggc tcgcgccccg tgcgcgtgct gggcaaccgg
     3181 cccgtggacg ccaaggcagc gctcggcaac cgcgaataca cactgatgta cgtgttgacg
     3241 tcgctcgaca aatacgccgg ctaccggatg tcggtgcggg cgtcgctgat ggaggggggc
     3301 aacggagccc tgaacgtgca gtcgatccag gccgtcagcc cgacctgcga gtaggcgact
     3361 ctgggccctc gtgcctccag agtcgcattg tcccgcgagt gattcagctt agaagctgta
     3421 aagggcgccc acggcgaacc ggaacatgtt gaccgcttcc tcctcgccag agacgcggag
     3481 atagtccccc ccggcacgga tgccgaatcc accggcaatc cggatgtcca caccgccgcc
     3541 cacctggatc gcgaagtctg ttgacgtgtc ggaaaaggac tctgactcga atatgggggg
     3601 gaaggcaggg gacgggggcg taaaggttat ggtcgaagtg aaggtccctt ccgacgacga
     3661 cttcacgacc ccgacgagga actgaccgaa gggagtgacc ctcccgccgc gagcgttgag
     3721 gcggacaccg cccatgtact ggttcaggct gaagtcctca tccacgatgt cagtgatagt
     3781 cgtcgctgga acaaagggcg gaaacccagg gtcggtgaag gtgttcgtct ccgaaaagga
     3841 cttggagttc ccgcccacct ggaaaacgat gccaattagc gacgtaacat tgccggcgac
     3901 atcgaagtac cagcctgtcg taaagaactc ctcgtcggcg atcacgccct gatatccacc
     3961 tgatacgtcg actctcggaa aatcctgggc gctcgccggg gcagaggcgg ccagcacgac
     4021 gaggctgacc agcacggaga cacttttcat acaactctcc tgtaagaccg ttcgtagatt
     4081 tgtttgcggg gtgcttgcta ggaaaacgac ccgccgaatc aggccgcaac gccgctatgg
     4141 aaggctggca ggcggactaa gctcagcaat attctcacat ctcgggggtc aatcccaact
     4201 cccaaccgag cccttgggag ttggaagttg ggagttggga gttgatcaga aggggaacac
     4261 cactccgacg ccgagacgga acgcgttgac accctcctca tcgccgaaga tgcggatgta
     4321 gc
//
LOCUS       DBNGSH010000312         2794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1553527, whole genome shotgun sequence.
ACCESSION   DBNGSH010000312 DBNGSH010000000
VERSION     DBNGSH010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1553527"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="AC16CY_07345"
     CDS             complement(<1..509)
                     /locus_tag="AC16CY_07345"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3353636.1"
                     /translation="MTEVVRVDPESPDPGVIERAAGCLRAGGLVAFPTETVYGLGVHA
                     LDRAAVRRLFEAKQRPSTDPLIVHVGSVEQLSSLASSVPESARALGSRFWPGPLTLVL
                     PRLPAVPDEVTAGLDTVAVRVPSHPIARALIAAAAVPVAAPSANLFSRPSATQASHVI
                     EDLDGRIDLV"
     gene            complement(524..1222)
                     /locus_tag="AC16CY_07350"
     CDS             complement(524..1222)
                     /locus_tag="AC16CY_07350"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR3353637.1"
                     /translation="MRTRIRMLAVTSVALIMFSLLVRADDSPDSAEASRIRESVVILE
                     EAMSARDAGIPASIIKKAEGIAVFPSLIKGGFFFGGMRGRGILSARTADGWSPPAFMT
                     LTGGSFGLQIGGQAADIVLVIMNRRGLEQFVRNQFKFGADASAAAGPVGRDAQATTDL
                     QLRAQILSYSRARGLFAGVTINGSTVRTDRDANERFYGKSLDGPDIVLDGTGIELRTP
                     VPEWVQALGKHAAP"
     gene            complement(1402..1821)
                     /locus_tag="AC16CY_07355"
     CDS             complement(1402..1821)
                     /locus_tag="AC16CY_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3353638.1"
                     /translation="MPQGLHATLTRRERQIMDVLFRRGRATAAEVMEELPGSPSYSTV
                     RTQLRVLEEKGYARHEEEGIRYVYTPAIRRHTARKAALKHLVDTFFEGSSEKAVAALL
                     GGEASRVSAEELDRIAELIAKARIVDLAQRAKKDNTR"
     gene            1942..2538
                     /locus_tag="AC16CY_07360"
     CDS             1942..2538
                     /locus_tag="AC16CY_07360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3353639.1"
                     /translation="MQLGLPFSDPAPTGAAGFAPTREAGLAPLYFVRHRRARRYLLRV
                     DHDGRVRVTIPRGGSRREADSFARRHTEWVARQRERLQRETTSPLDRSAGQAQAQVEL
                     PSRLYELAAQLGLTVRRVSVRNQRTRWGSCGRDGHICLNWRLVVMPPWVRDYVIVHEL
                     MHLERLDHSPAYWRLVAAACPAYRDARQWLRIHGPSLR"
     gene            2571..>2794
                     /locus_tag="AC16CY_07365"
     CDS             2571..>2794
                     /locus_tag="AC16CY_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3353640.1"
                     /translation="MNRRQVIVALVGAGVAGPAVFWLASTTETSATGEQLFPIRKSDG
                     EWRAALTPDQYRVLRGHATERAFTSPLNAE"
BASE COUNT          454 a          921 c          970 g          449 t
ORIGIN      
        1 acgaggtcga tgcggccgtc gagatcctcg atcacgtgcg acgcctgcgt ggcgctcggc
       61 ctggagaaga gattggcgct cggcgcggcg acgggaacgg cagcggccgc gatgagcgcg
      121 cgggcgatcg gatgcgatgg cacgcgcacg gcgacggtat ccaaccctgc ggtgacttca
      181 tccggcaccg ccggcaaacg gggaagcacg agcgtgagag gtcctggcca gaagcggctg
      241 ccgagcgcac gggcggattc gggcacggac gaggcgaggg acgagagctg ttcgaccgag
      301 ccgacgtgaa cgatgagcgg atcggtggac ggccgctgct tggcttcgaa caggcgccgc
      361 accgcggcgc ggtcgagcgc gtggacgccg aggccgtaca ccgtctcggt cggaaacgcg
      421 acgagtcccc cggctcgaag gcagccggcg gcacgctcga tgacgcctgg atccggactc
      481 tccggatcga cccgaacgac ttcggtcacc agtcgcgtac ccgtcagggc gcagcgtgct
      541 tgccgagcgc ctgcacccac tcggggactg gcgttcgcaa ctcgatgccc gtgccgtcga
      601 gcacgatgtc cgggccatcg aggctcttgc cgtagaaacg ctcgttggcg tcacggtcgg
      661 tgcgcacggt gcttccgttg atggtgaccc cggcaaagag ccccctggct cgcgaatagc
      721 tgaggatctg tgcgcgcagc tgcaggtccg tcgtggcctg cgcgtcgcgc ccgaccggtc
      781 ctgcggccgc cgacgcgtcg gcgccgaact tgaactggtt gcggacgaac tgctcgaggc
      841 cgcgccggtt catgatgacg aggacgatgt cggctgcctg accgccgatc tgaagcccga
      901 agcttccgcc ggtcagcgtc atgaacgcgg gaggcgacca gccgtccgcg gttcgcgcgc
      961 tcaggatgcc gcggccccgc atcccgccga agaagaagcc gcccttgatc aggctcggga
     1021 agacggcgat accctcggcc ttcttgatga tggacgccgg aatcccggca tcccgcgccg
     1081 acatggcttc ctccagaatc accacagatt ctcgaatccg gctggcctcc gccgaatcag
     1141 gcgagtcgtc tgcacgaacg aggagggaga acatgatcaa cgcgaccgac gtgaccgcga
     1201 gcatgcgaat gcgcgttctc atgccccaat gatagccgca cggcggcggc tgggcttggg
     1261 ccgccgagct tgtgtcaacg cggcgggcga tactggaact ccatcgtgat ccggatgttc
     1321 gtttcaaccg gaacgccgtt cagcagtgtc gggctgaact cccactgacg caccgcttcg
     1381 ctcagtccct ggcgctcgcg gtcatcgggt gttgtctttc ttggcccgct gggccaggtc
     1441 gacgattcga gccttggcga tcagctcggc gatccggtcc agctcctcgg ccgagacgcg
     1501 ggatgcctcg ccgccgagca gcgcagcgac cgccttttcc gacgagccct cgaagaacgt
     1561 gtcgaccagg tgcttgagcg cggccttgcg ggccgtgtgg cggcggatcg cgggcgtgta
     1621 gacgtagcga atgccctctt cctcgtgacg cgcgtacccc ttctcttcga gaaccctgag
     1681 ttgcgtccgc acggtggagt agctggggga gcccggcagc tcctccatca cctcggcggc
     1741 cgtcgcccgg ccgcggcgga agagcacgtc catgatctgc cgctcacgcc tggtcagggt
     1801 ggcgtgcagg ccctggggca tgtgttatgt acctcgcaga tgtgttatag aaataacata
     1861 caggcatgac gtcgtgtcaa gtggctcgtc gcggggcggc gctggtgggc cgaggcggcg
     1921 gaatcgggta tcctgtcgat cgtgcagctc ggtctgcctt tcagcgatcc cgcaccgaca
     1981 ggcgcagccg ggtttgcacc gacacgcgaa gcggggctcg caccgttgta tttcgtccgc
     2041 caccgccgag cccggcgcta ccttctgcgc gtcgatcacg acggccgcgt gcgcgtgacg
     2101 attccgcgcg gcggctcacg ccgcgaggcc gactcgttcg cgcggaggca taccgagtgg
     2161 gtggcgcgtc aacgcgagcg gctgcagcgc gagacgacct cgcccctcga cagaagcgcg
     2221 gggcaggcgc aggcgcaggt cgagcttccg tcccgcctgt acgagcttgc cgcacagctt
     2281 ggcctcacgg tccggcgggt cagcgttcga aatcaacgca cccgctgggg atcctgcggc
     2341 cgcgacgggc acatctgcct gaactggagg ctggtcgtga tgccaccttg ggttcgcgac
     2401 tacgtcatcg ttcacgagct catgcacctc gaacggctcg atcattcgcc ggcatactgg
     2461 cggctcgtgg cggcggcctg cccggcttac cgcgacgcgc gtcaatggct caggatccac
     2521 ggcccctcgt tgcggtaagg tagaagaaga cgcggaacag gtcggagccc atgaatagac
     2581 gtcaagtcat cgtcgccctc gtcggcgcag gcgtcgcagg gccggccgtc ttctggctgg
     2641 ccagtacgac ggagacgagc gcgacgggcg agcagctgtt tccgatccgc aagagcgacg
     2701 gcgagtggcg tgcggcgctg accccggatc agtacagggt tctgcgaggg cacgccaccg
     2761 agcgggcgtt cacgagccca ctgaacgccg agaa
//
LOCUS       DBNGSH010000313         4465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2741916, whole genome shotgun sequence.
ACCESSION   DBNGSH010000313 DBNGSH010000000
VERSION     DBNGSH010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2741916"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2691
                     /locus_tag="AC16CY_07370"
     CDS             <1..2691
                     /locus_tag="AC16CY_07370"
                     /inference="COORDINATES: protein motif:HMM:NF021606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HIR3353641.1"
                     /translation="GAVGAAFGLSTALLGFAVGLLAGLLTETRNRQASLHDQIREIRE
                     QNASLELKLLALERRATTPAAAAPPPVAPEQTAPWTPPPLRAHAETPSPPGPPPPPPE
                     QPHEAPAPAEATPIPRPIDRPEIAPPALQWFVEFFTTGNVVAKVGMIVVFFGVAFLVR
                     YASERGWLPIEFRLMGVAAGSLVLLGVGWRLRHSRPEYGVVLQGGAVGVLYLTIFAAF
                     RLYELLPPPLTFGLLLMVVAFSGMLAVAQDTVSLAVLGASGGFLAPILASTGGGSHVA
                     LFSYYAVLNAGVLGIAWFRSWRFLNWLAFVFTFGIGFVWGAQYYQPAMLGTTEPFLVF
                     FFLLFVAVSILSAHRLPPQLRGYIDGTLVFGTPAVAFGMQSVLVRDVPLGRAYSAVAV
                     SALYLVLARLVWARDSVLRPLGEAFLALAVVFLVVAVPLAFDGHATAAAWALEGAGLV
                     WIGVRQDRLLARLAGSLLPIGAGVAFAVMTTSPAGAMPVLNTQFLGCAAIAAGSLVCG
                     QFLSKAREGRSRVESTLEWVVLVWGLLWWFGGALAEIDRHVPSRLSYAAAYLTFAASL
                     CAIGGLARWWQWRPMMHAALPIGPLMWASVLFVYLDRADAGPLTDLGWLTWPVVVASS
                     YLVMYWFESIWPPLLVSAWHAGASWLIVFLATWTAVASVARLVPETPTWAWTLWAVVP
                     AIFVLAIRSLGALVAWPIRRFQSLYEGPISFVPVAGILLWVLWASGQSGEAAPLPYVP
                     IVNPIELTQVLGLIVSYVWWRDSRGDVAEEIGRTFALALAVLAFIALNVLVARAVHFY
                     GGVRFDPDDLVESAIFQTGISILWAVTAGTLMTLALRRASRPVWVTGASLLAALILKL
                     FVVDLGNVGGVARIVSFLATGLLILVIGYFAPLPPKTERA"
     gene            2688..4031
                     /locus_tag="AC16CY_07375"
     CDS             2688..4031
                     /locus_tag="AC16CY_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HIR3353642.1"
                     /translation="MTQFAWLLALIVSLPAVIVAQTTLNDFARGAEIRAEEGGTIFRV
                     LLPDDVYDTTTRTDLADLRVINGAGESVPHALRGTPRPPADSEWRDVPSFPMKEQTGV
                     AATTQVRIGRDGSVLAVTGDSTGGRVTTSYLLDASALEAPIDRVALAWEAGPGVTFLA
                     PVSVQGSDDLNRWRTIVASAAVAQLQRDGFGLSQNEIGLPASSERIKYLRISWPKEMS
                     AVVVRSARVRLSAGVPRPEIRWKTLSAGEVDAAGVAYYDARATAPVQYIDLELADPTD
                     VATVTVRSSGNPSSAWVTRHVGLFYSLQQPGGTIRSEFARSSPSMDRYWRLETTREGG
                     WKKDRAPRLKVGWHPLELVFLAQGPRPYTLAYGSSRVGPSEAPVDSLLASLDEADRAS
                     QVRLATVGESRTLGGPAALEPVLPWRRIALWGVLVAAVAALAFLATRVLRETKQA"
     gene            complement(4033..>4465)
                     /locus_tag="AC16CY_07380"
     CDS             complement(4033..>4465)
                     /locus_tag="AC16CY_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3353643.1"
                     /translation="LQPGKTFSVRQAMEPLAPGEVEPEPPVPAEKPADARPEQSRTRP
                     GRPPARSRDQVDVEPEPAPPSPSRPEFGAISLRVQPDDASITIDGERWENAPVNERLI
                     VQLGTGVHNVEIRRDGYRTYVTDITVRPGQTATINVSLTKQ"
BASE COUNT          551 a         1506 c         1553 g          855 t
ORIGIN      
        1 ggagcggttg gtgcagcgtt cgggctgagc accgcactcc tcggcttcgc tgtcggtctt
       61 cttgcggggc tgctgaccga gacccgcaat cgccaggcat cactccacga tcagatccgc
      121 gagattcgcg aacagaacgc gagcctcgaa ctgaagcttc tcgcgctcga gcgccgcgcg
      181 accacacctg ctgcagccgc tccgccgccg gtcgctcctg aacagaccgc tccgtggact
      241 cctccgccac tgcgggcgca cgccgagact ccgtcgccgc cgggtcctcc gccgccaccg
      301 ccggaacagc cgcacgaggc acccgcgccc gcggaagcga cgccgatccc gcggcctatc
      361 gacaggcccg aaattgcgcc tcccgcgttg cagtggttcg tcgagttctt cacgaccgga
      421 aacgtggtgg ccaaggtcgg catgatcgtc gtgttcttcg gcgtggcgtt cctcgtgcgc
      481 tacgcatcgg aacgcggctg gctcccgatc gagttccgcc tgatgggcgt cgctgccggt
      541 tcgctcgtcc tgctgggcgt gggctggcgc ctcaggcact cgcggcccga gtacggcgtc
      601 gtgctccagg gcggcgccgt cggcgtcctg tacttgacga tcttcgcggc gttccgtctg
      661 tacgagttgc tgccgccgcc gctcacgttc ggactcctgc tcatggtggt cgccttcagc
      721 ggaatgctgg ccgtggcgca ggacaccgtc tcgcttgccg tgctcggcgc gagcggcggg
      781 ttcctggcgc ccattctcgc gtccaccgga ggaggcagcc acgtcgcgct cttctcgtat
      841 tacgcggtcc tcaacgccgg cgtgctcggc atcgcgtggt tccgaagctg gcgcttcctg
      901 aactggctgg cgttcgtctt caccttcggc atcggcttcg tgtggggtgc gcagtactac
      961 cagccggcga tgctgggcac gaccgagccg ttcctcgtgt tcttctttct gctgttcgtt
     1021 gcggtgtcaa tcctgtccgc gcaccggctg ccgccgcagc tgcgcggcta catcgacgga
     1081 acgctggtct tcggcacccc agcggtcgcc ttcggcatgc aaagcgtcct cgtccgcgac
     1141 gtgccgctcg ggcgcgcgta ttcggccgtt gccgtcagcg cgctgtatct ggtccttgcc
     1201 cgcctcgtgt gggcccgcga ttcggtgctg cgtcccctcg gcgaggcgtt tctcgcgctg
     1261 gccgtcgtgt tcctcgtcgt cgcagtgccg ctggccttcg acgggcacgc caccgcggcc
     1321 gcgtgggcgc tcgagggcgc gggcctcgtg tggatcggcg tgcgccagga tcgcctgctc
     1381 gcgcggctgg ccggatccct cctgccgata ggcgccggcg tggccttcgc cgtcatgacg
     1441 acgtcgccgg ccggggccat gccggtgctc aacacgcagt tcctcggctg cgcggcgatt
     1501 gcggccggca gcctcgtctg cgggcagttc ctgtcgaagg cacgcgaggg acgctcgagg
     1561 gtcgaaagca cgctcgaatg ggtggtgctc gtctggggct tgttgtggtg gttcggaggc
     1621 gcgctggccg agatcgatcg ccacgtcccg tcgcggttgt cgtacgcggc ggcgtacctg
     1681 accttcgccg cgagcctctg cgcgattgga ggccttgccc gatggtggca atggcgcccg
     1741 atgatgcatg cggcgctgcc gatcgggccg ctcatgtggg cctcggtgct gttcgtctac
     1801 ctcgatcgcg cggatgccgg gcctctgacc gaccttggct ggctcacgtg gcccgtcgtg
     1861 gtcgcgtcga gctacctggt catgtactgg ttcgaatcga tttggccgcc gcttctggtc
     1921 agcgcgtggc acgcgggggc atcgtggctc atcgtctttc tggccacgtg gacggcggtg
     1981 gcgtccgtcg ctcgactcgt acccgagacg ccgacgtggg cgtggacgct gtgggccgtc
     2041 gttcctgcca tcttcgtact ggccattcgc agcctcggcg ccttggtggc atggccgatt
     2101 cgccgcttcc agtcgttgta cgagggcccg atctcgttcg ttcccgtcgc tggcatcctg
     2161 ctgtgggtgt tgtgggcctc gggccagagc ggggaagccg cgccgctgcc gtacgtgccc
     2221 atcgtgaatc cgatcgagct gacccaggtg ctcggcctca tcgtgtcgta cgtgtggtgg
     2281 agggattcgc gtggtgatgt ggcagaggaa atcggccgga ccttcgctct ggcgcttgcc
     2341 gtcctggcgt tcatcgcgct gaacgtgctc gtcgcccgcg ccgtgcattt ctacggcgga
     2401 gtgcggttcg atcccgacga cctggtcgag tccgcgatct tccagacggg catttccatc
     2461 ctctgggccg tgacggccgg caccctcatg acgctggcgc ttcgccgagc gagccggccg
     2521 gtgtgggtga ccggagcgtc gctgctcgcc gcgctcatcc tcaagctgtt tgtcgtcgat
     2581 ctcggtaatg tcggcggtgt cgcgcgcatc gtgtcgttcc tggcgaccgg cctcctcatc
     2641 ctcgtgatcg gctacttcgc gccgctgcca cccaagacgg agagagcatg acgcagtttg
     2701 cgtggcttct cgccctgatc gtgtcgttgc ccgctgtcat cgtggcccag acgacgctga
     2761 acgacttcgc gcgtggcgca gagattcgag ccgaggaggg cggcaccatc tttcgcgtgc
     2821 tgctcccgga cgacgtgtac gacaccacga cgcggacgga cctggcggat cttcgagtca
     2881 tcaatggcgc cggagagtcc gtgcctcatg ccttgcgcgg gacgccgcgt ccgccggcgg
     2941 attccgagtg gcgggacgta ccgtcattcc ccatgaaaga acagacgggc gttgccgcga
     3001 ccacgcaggt gagaatcggc cgggacggat cggtgctcgc ggtcacgggc gacagcaccg
     3061 gcggccgcgt cacgacctcg tatctgctgg atgcgagcgc cctcgaggcc ccgatcgacc
     3121 gggtggcgct tgcatgggag gccgggccgg gcgtgacctt tctcgcaccg gtcagcgttc
     3181 aggggtccga cgatttgaac cgctggcgga cgatcgtcgc ctcggccgcc gtcgcacaac
     3241 tgcagcgcga cgggttcggc ctgtcacaaa acgagatcgg gctgccggcg tcgagcgagc
     3301 ggatcaagta cctgcgcatc tcgtggccga aggagatgtc ggccgtcgtg gtgaggtcgg
     3361 cgcgcgttcg gctgtcggcc ggagtccctc gcccggagat tcgatggaag accctgagcg
     3421 ccggggaggt ggatgcggca ggcgtggcgt actacgacgc tcgcgcgacg gcgcccgtgc
     3481 aatacatcga tctcgagctt gccgatccca cggacgtcgc gaccgtcacg gtgcgctcca
     3541 gcggcaatcc gtcgtcggcg tgggtgacgc gtcatgtcgg gctgttctat tccctgcagc
     3601 agccgggcgg cacgatacgc agcgagttcg cgagatcgtc tccgtcgatg gatcgctact
     3661 ggaggctcga gaccacgcga gaaggcggtt ggaaaaagga ccgcgcgccg cggctcaagg
     3721 taggctggca tccgctcgag ctggtgttcc tggctcaggg gccgcgaccc tacacgctcg
     3781 cgtacgggag ctcgcgagtg ggtccgtcgg aggcaccggt ggactcgctc cttgccagcc
     3841 tggacgaggc ggaccgcgcc agccaggtcc ggctggcgac ggtgggcgag tcgcgcaccc
     3901 tgggcggccc cgccgcgctc gagcccgtgc tgccgtggcg ccggatcgcg ttgtggggtg
     3961 tgctcgtggc ggccgtcgcc gcgctcgcgt tcctggcaac gcgagtcctg cgcgagacga
     4021 agcaggcgtg agctattgct tggtgagtga aacgttgatc gtcgccgtct gacccggacg
     4081 cacggtgatg tccgtgacgt aggtacggta accgtccctc cggatctcca cgttgtgcac
     4141 gcccgtgccc agctgcacga tcagccgttc gttcaccgga gcgttctccc accgctcgcc
     4201 gtcgatcgtg atcgaggcgt cgtctggctg cactcgcagc gagatcgcac caaactcggg
     4261 cctcgatgga gacggcggcg cgggttccgg ttccacgtca acctgatcgc gcgagcgggc
     4321 cggcggccgg cctggccgcg tgcgggattg ctccggcctg gcgtcggccg gtttctcggc
     4381 cgggactggc ggctcgggct ccacttctcc aggggcaagc ggctccatcg cctgccgcac
     4441 gctgaacgtc ttgcccggtt gcaag
//
LOCUS       DBNGSH010000314         2620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3930342, whole genome shotgun sequence.
ACCESSION   DBNGSH010000314 DBNGSH010000000
VERSION     DBNGSH010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3930342"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(611..1159)
                     /locus_tag="AC16CY_07385"
     CDS             complement(611..1159)
                     /locus_tag="AC16CY_07385"
                     /inference="COORDINATES: protein motif:HMM:NF039330.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HIR3353644.1"
                     /translation="MSAEQLREPWRSFLHDIDEHLAGPTEIHCLGGFVVAEYYGLARP
                     TADVDVIHVRGESSVADVQRIGGKGSALARKHRVYIDIVTVADVPERYEERLIDAYTG
                     AFKNLRVKVFERHDLALAKLGRNQDYDREDVRRLAQGPGLDVAVLEQRYREELRWRLG
                     KPGREDLTLELWVEMIRELQNR"
     gene            complement(1149..1826)
                     /locus_tag="AC16CY_07390"
     CDS             complement(1149..1826)
                     /locus_tag="AC16CY_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3353645.1"
                     /translation="MTGADVRTARERRGWQQRELADRLGVSQGYVSLLESGRRPVSKR
                     LTAKLVSLLSLSPSVLPMPTPKPLPNQQVARALGRLGYPGFAYLRGSATVNPMEVLLG
                     ALLADDVNARVVEGLPWLLLRYPDVDWERLVMYAKQHDAQNRLGFLVALAHRLAVANG
                     DAAAAELLGRRERALQRSLLQHDDTFSRTSLTQAERRWLVTNRSPEAERWNLLATLNV
                     DRLLNVG"
     gene            1825..2016
                     /locus_tag="AC16CY_07395"
     CDS             1825..2016
                     /locus_tag="AC16CY_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353646.1"
                     /translation="MSIYYIMCNIRDEAFGESLRDQLNRQLAMIDPFLTFSWADWKNV
                     AIFVLENDVDFQQARSSSQ"
     gene            2055..2492
                     /locus_tag="AC16CY_07400"
     CDS             2055..2492
                     /locus_tag="AC16CY_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3353647.1"
                     /translation="MRVMVFFKNDMDNEGMAPTPEMLEAFKKMDEFTEELVKAGVFVA
                     GAGLKPSAQSKRVVFDGRGRSTVIDGPFAEARELVAGFSIWEVKDMDEAMAWVARCPN
                     TLPDRTGEIEIRPFYEAADLADFVTPEELATPRTGERGKLGVA"
     gene            2492..>2620
                     /locus_tag="AC16CY_07405"
     CDS             2492..>2620
                     /locus_tag="AC16CY_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HIR3353648.1"
                     /translation="MHQAIEATFRIERARLIAGLARVVRNVDLAEELAQDALVVALA"
BASE COUNT          475 a          861 c          804 g          480 t
ORIGIN      
        1 tcaccaagga ccgtgacgta actgtgacgt gaccctgtcg attccgcgag atctactgag
       61 gtttacggcg atcgatggag tagacgtgat cggcgttaag tcgttggctt cgcaacggta
      121 atcgaaatct gcagcacgcc atcgcagacc gacagagcaa tttccaagcg tgctccttca
      181 accactcgga catctctccc gtcatgcgac gctcgcgtgg cgtcctgtca gtatctcacg
      241 tggccccggc ggcatgacgc gggcgagcac gtagggaatc aggacaccga ggttgccgag
      301 aaccggcatg agcctccgcg ccgcggccgc gaacggcgat cgacccgatc gccttgcccg
      361 acctgcgtgt tcaccagatc cgagagcccg gacgatcagc agccggacgt cgcgccgccg
      421 aggttgatca ggacttcagg ccgcccccgc tcgatcgcgt gctcggttga cgacgccgcc
      481 gcgatcttcc gccagcggcc gtgggagaac aggacgtcct cgttcccgac ggcacgcgtg
      541 aagcactcac cgtggggcgt cgggcgtcgg ctcgacgcgt tccgggttca cctgcgcccg
      601 ctgcgcgggc tcaacggttc tgtagctccc tgatcatctc gacccagagc tcgagcgtca
      661 ggtcttcgcg ccctggcttt cccaaccgcc atcgcaactc ttcgcggtat cgctgttcga
      721 ggactgccac gtccaagccc ggaccttgcg cgagacgacg gacgtcctcg cggtcgtaat
      781 cctgattgcg accgagcttt gccagagcca ggtcgtgtct ctcgaatact ttgaccctca
      841 agttcttgaa cgctccggta tacgcatcga tcaatcgctc ttcgtatcgc tccggaacat
      901 cagcgaccgt cacgatatcg atatagaccc gatgcttcct ggcgagcgca ctgcctttgc
      961 ccccgattcg ttgcacgtct gcgacactcg attcgccgcg gacgtgaatg acatcgacgt
     1021 ccgcggtcgg ccgcgccaag ccgtagtact ccgcgaccac gaatccaccg aggcagtgaa
     1081 tctccgtcgg ccccgcgaga tgctcgtcga tgtcgtggag gaacgaccgc cacggttcgc
     1141 ggagttgctc agccgacatt caagagccga tcaacattca gcgtcgcgag cagattccac
     1201 cgttcggcct ctggagaccg gttggtcacg agccatcgcc gttcggcctg tgtgagagag
     1261 gttctcgaaa acgtgtcgtc atgttgcaga agcgatcgct gcaatgcccg ctcgcgtcgt
     1321 cccagcagtt ccgctgcggc agcatctccg ttcgccacgg ccaagcgatg cgccagcgcc
     1381 acgaggaatc ccaaccggtt ctgtgcgtcg tgctgcttcg cgtacatcac gagccgctcc
     1441 cagtccacat cgggataccg aaggagcagc cacggcaggc cctcgacgac acgcgcgttg
     1501 acatcgtcgg cgagcagggc cccgagcaac acttccatgg gattcacggt ggcgctgccg
     1561 cgaagatagg cgaagcccgg ataccccagg cgccccagtg cgcgagcgac ctgctggttc
     1621 ggcagcggct ttggtgtcgg catcggtaga acgcttggcg acagcgagag gagcgacacg
     1681 agcttcgccg tgaggcgttt cgagacaggc cggcgaccgc tctccagcag agacacgtat
     1741 ccctgcgaca ctcccaatcg gtccgccaac tcacgttgtt gccagccgcg gcgctcgcgc
     1801 gctgtgcgga catccgctcc cgtcatgtcc atatattaca tcatgtgtaa tattcgcgac
     1861 gaggcgtttg gcgagtcgct tcgggaccag ctcaaccggc agctcgcgat gatcgacccc
     1921 ttcctgacct tctcctgggc tgactggaag aatgtggcca tatttgtgct ggagaacgat
     1981 gtcgatttcc agcaagctcg ttcgtcgtcc caatgagacg ccaattgggg ccggctcgaa
     2041 caaggagaga ggcgatgcgt gtgatggtgt tcttcaaaaa cgatatggac aacgaaggca
     2101 tggcccccac gcccgagatg ctcgaggcgt tcaagaagat ggacgagttc accgaggaac
     2161 tggtcaaggc cggcgtcttc gtggccggcg ccggcctcaa gcccagcgcc cagtccaagc
     2221 gcgtcgtctt cgatggtcgt ggccgcagca cggtcatcga cgggccgttt gccgaggccc
     2281 gcgagctggt cgccggcttc tcgatctggg aggtcaagga catggacgag gccatggcct
     2341 gggtggcgcg ctgtcccaat accctgccgg accgcacggg cgagatcgag atccggccgt
     2401 tctacgaggc ggcggatctg gcggatttcg tgacgcccga ggaactcgca acgccccgta
     2461 ccggtgagcg cgggaagctc ggcgtcgcct gatgcaccag gcaatcgaag cgacctttcg
     2521 cattgaacgg gccaggctca tcgcgggcct cgcccgcgtg gtgcgcaatg tcgaccttgc
     2581 cgaggagctc gcgcaggacg cgctcgtcgt cgccctcgcc
//
LOCUS       DBNGSH010000315         3392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2402423, whole genome shotgun sequence.
ACCESSION   DBNGSH010000315 DBNGSH010000000
VERSION     DBNGSH010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2402423"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="AC16CY_07410"
     CDS             <1..221
                     /locus_tag="AC16CY_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353649.1"
                     /translation="LGTAHTNPVFIRVGGRPIRASRRSAEWCLKAVDQCWSQKAPRIS
                     AGERDEAQRAYDVARTRYRQIVSESDID"
     gene            complement(348..1523)
                     /locus_tag="AC16CY_07415"
     CDS             complement(348..1523)
                     /locus_tag="AC16CY_07415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR3353650.1"
                     /translation="MRSEASLARAVAFPRVINIEDLRRLARKRLPSLVFAYIDGGAED
                     EITLRENVRAFDDVTFRPRQCIAVPDCDLRTTVLGTTIDLPFLLAPVGFCRMFHPRGE
                     ECAARAASTAGTGYVLSSFSGTRLEDVRESARGLLWYQLYVPGGRAVAEATIARAKAA
                     GYEALVVTIDTPVAGMRERDYRYGVRPLLQGRFWSSLPHAWQFVTRPGWVLGYLSDGA
                     VRVFPNVELPGVGPMPCSDVGVLLEETVVTWKDLRWMHDAWKGPIVVKGVHTGDDARR
                     AIDAGASAVVVSNHGGRQLDGVPASLRALPEVVEAVNGRVDVLMDGGIRRGSHVVKAL
                     CLGARAVLVGRAYAWGLGAAGGPGVARAIEILRADIVRTMKLLGCGAIKDLDRSYLN"
     gene            complement(1528..2373)
                     /gene="bla"
                     /locus_tag="AC16CY_07420"
     CDS             complement(1528..2373)
                     /gene="bla"
                     /locus_tag="AC16CY_07420"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HIR3353651.1"
                     /translation="MRTHHIVVLGIVLLASGAGLEGQGRGQQNFNGPFPPHKIIGNVY
                     YVGSDQLASFLITTPEGHILVNSSFETTVPVIRAAVEKLGFKFEDIRILVGSHAHGDH
                     MDGDALVKELTRAQVMAMEQDVPALRRMQPGGKPHPIDRVLRDGDEVKLGGTTMVARL
                     TAGHTPGCTTWTLKAQEGGRSYDVVISGSIGVNPGYVLVGNKDYPQIADDYVRSFKVM
                     RALPVDVFLGAHGAFYGLTGKYAQLEKGGPNPFVDPEGFKAYVDRSEQAFLTMLEGQK
                     KAATK"
     gene            complement(2418..>3392)
                     /locus_tag="AC16CY_07425"
     CDS             complement(2418..>3392)
                     /locus_tag="AC16CY_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3353652.1"
                     /translation="QDARRTTAATQPFPAGADSALPDCAHWKQQPLPQGFDLGCDFYT
                     PASVDKPNLLTPTFGMRVAPMAYSPDTGYFYAAGNASLGWLRRDENPWFFSNGFNQRV
                     PGLNRLSHGLLAAIDSRTNKVVWKKEFRPGRPTGAMTTAGGLMFQSAPDGNLQAYDAR
                     TGNLLWQFQLGAAGGPPLTYEIGGEQYVAAVSSAGVWVLKLGGTVPQAAAPARPPQPE
                     LFGGQITDTTQIETASLVRDTGFTGFHYMTDEYAFSPYRARVKRGAQVTWRNNGLMVH
                     TIVAQDGSWTTGPLNPADVGGVTFDKPGSHAYMCKEHPWAVGQIVVTE"
BASE COUNT          537 a         1181 c         1098 g          576 t
ORIGIN      
        1 tcctcggcac ggctcacacg aaccccgtct tcattcgcgt cggcggccgg ccgatccgcg
       61 cgtcgaggag aagcgcggag tggtgcctga aggcggtcga tcagtgctgg tcgcagaagg
      121 cgccccgcat cagcgcaggg gaacgcgacg aggcgcagcg cgcctacgac gtcgcgcgca
      181 ctcgctaccg gcagatcgtg tcggagagtg acatcgacta gatcgatcgt agtgtccggc
      241 ttcagccgga ccttgaccgc gacgtacgta gtgtccggct tcagccggac cttgaccgca
      301 acgtacgtag tgtccggctt cagccggacc gtcgctggcg caatccgtca gttcagataa
      361 gaccggtcga gatctttgat cgcgccgcag ccgagcagct tcatcgtccg cacgatgtcg
      421 gcgcgcagga tctcgatggc acgggcgacg cccgggccgc cggccgcgcc gagtccccac
      481 gcgtaggccc ggccgacgag cacggcgcgg gcgcccaggc agagcgcctt caccacgtgg
      541 ctgccgcgcc ggatgccgcc gtccatgagc acgtctacgc ggccgttcac ggcctcgacg
      601 acctcgggca gcgcacgcag cgacgccggc acgccatcga gctggcgccc gccgtggttc
      661 gatacgacca ccgcgctcgc cccggcgtcg atcgcgcggc gcgcgtcgtc tcccgtgtga
      721 acgcccttga cgacgatcgg gcccttccac gcgtcgtgca tccagcgcag atccttccag
      781 gtcacgaccg tctcttcgag gagcacgccg acgtcgctgc acggcatcgg gccgacgccg
      841 ggcaactcga cgttgggaaa caccctgacg gcgccgtcac tgaggtagcc cagcacccat
      901 ccggggcgcg tgacgaactg ccacgcgtgc ggcaggctcg accagaagcg cccctgcagc
      961 agcggcctga cgccataccg gtaatcgcgc tcgcgcatgc cggccaccgg agtatcgatc
     1021 gtcacgacga gcgcctcgta cccggccgct ttcgcgcgcg cgatcgtcgc ctcggcgacc
     1081 gcgcggccgc ccgggacgta gagctgatac cacagcaggc cgcgagcact ttcgcggacg
     1141 tcctcgagcc gcgtccccga aaacgacgag aggacgtagc ccgtgccggc ggtgctcgcc
     1201 gcgcgggcgg cgcactcctc tccgcgtgga tggaacatgc ggcagaagcc gaccggggcg
     1261 agcaggaacg gcagatcgat ggtcgtgccg agaaccgtcg tccgcaggtc gcagtccggc
     1321 acggctatac actggcgcgg acggaacgtc acgtcgtcaa acgccctgac gttttcacgg
     1381 agcgtgatct cgtcctccgc gccgccgtcg atgtacgcga agaccaggga cggcaggcgc
     1441 ttccgggcga ggcgcctgag gtcctcgatg ttgatcacgc gcgggaacgc gacggcgcga
     1501 gccaggctcg cctcggatcg catcgcgcta cttcgtcgcg gccttcttct gtccctcgag
     1561 catggtgagg aacgcctgtt cgctccgatc cacatacgct ttgaagcctt cgggatcgac
     1621 gaacggattc ggaccgccct tttcgagctg tgcgtacttt ccggtcaggc cgtagaaagc
     1681 tccgtgtgcg ccgagaaaca cgtcgaccgg aagggcgcgc atcaccttga agctcctcac
     1741 gtagtcgtcg gcaatctgcg ggtagtcctt gttgccgacg agcacgtacc cagggttgac
     1801 gccgatgctg cccgagatga cgacgtcgta cgagcggccg ccctcttgcg ccttcagcgt
     1861 ccacgtcgtg cagcccgggg tgtgtcccgc cgtcaggcgg gccaccatgg tcgttccacc
     1921 cagcttcacc tcgtctccgt cgcgcagcac gcgatcgatc gggtgcggct tcccccccgg
     1981 ttgcatcctg cgcagcgcgg gcacgtcctg ttccatggcc atcacctgcg ccctggtcaa
     2041 ctccttgacg agcgcgtccc cgtccatgtg atcgccgtgg gcgtggctgc cgaccaggat
     2101 cctgatgtcc tcgaacttga atccaagttt ctcgactgcg gcacgaatca ccggaaccgt
     2161 cgtctcgaag ctgctgttga cgaggatgtg gccctccggc gtggtgatga ggaaagacgc
     2221 cagctgatcc gaccccacgt agtagacgtt gccgatgatc ttgtgcggcg ggaatggccc
     2281 gttgaagttc tgctgtcccc gtccctgccc ctcgagcccc gcgccggagg cgaggagcac
     2341 gatgcccagg accacgatgt gatgcgtccg catgaccttc accctcgagt cttgagtctt
     2401 gagttttgag ttgtgaccta ctcggtcacc acaatctgcc ccaccgccca cggatgctcc
     2461 ttgcacatgt aggcgtggct gcccggcttg tcgaacgtga cgccgccgac gtcggccggg
     2521 ttgagcggcc cggtcgtcca cgatccatcc tgcgccacga tcgtgtgcac catcaggccg
     2581 ttgttgcgcc acgtcacctg cgcgcctcgc ttcacgcgcg cccggtacgg gctgaaggcg
     2641 tattcgtccg tcatgtagtg gaatcccgtg aacccggtgt cgcggacgag cgaggccgtt
     2701 tcgatctgcg tcgtgtcggt gatctggccg ccgaacagct ccggctgcgg cggacgggcg
     2761 ggtgccgccg cctgcggcac cgtgccgccc agtttgagca cccacacgcc ggccgacgaa
     2821 accgctgcga cgtactgctc gccgccgatc tcgtacgtca gcgggggtcc gccggcggct
     2881 cccagctgaa actgccacaa gagattcccg gtcctcgcgt cgtaggcctg caggttgccg
     2941 tctggcgccg actggaacat caatccgccg gccgtcgtca tcgcgccggt gggccggccc
     3001 ggtcgaaact ccttcttcca cacgaccttg ttcgttcggc tgtcaatggc agccagcagc
     3061 ccgtgactga gacgattcag gccaggcacc cgctggttga agccgttgct gaagaaccag
     3121 ggattctcat cgcgccgcag ccagccaagc gacgcgttgc cggccgcgta gaagtatccg
     3181 gtgtctggac tgtaggccat gggcgcgacg cgcatgccga aagtcggcgt gagcagattc
     3241 ggcttgtcca ccgatgcggg cgtgtagaag tcgcatccga gatcgaaacc ctgcggcagc
     3301 ggctgttgtt tccaatgggc acagtcgggc aacgcgctgt cggcgcccgc aggaaacggt
     3361 tgcgtcgccg cggtggttcg acgcgcgtcc tg
//
LOCUS       DBNGSH010000316         3604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_704991, whole genome shotgun sequence.
ACCESSION   DBNGSH010000316 DBNGSH010000000
VERSION     DBNGSH010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_704991"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1348)
                     /locus_tag="AC16CY_07430"
     CDS             complement(203..1348)
                     /locus_tag="AC16CY_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353653.1"
                     /translation="MTTRMIGSAVTLAACTAVGALMSVPVSGQTAQATQASVAPPALV
                     VTAFGGAPITYKAPRTPWGDPDLQGVWASDDMAGIPMSRPAQYGDRLFLNEQELAARK
                     KQIESGVARSENEADSTFRFDFARRAFPQTSQVVDPPNGQMPATLPGRRQMPRGTFGP
                     GPLDWVTDFSGYERCITRGIFGSALNVIYGNGNRIVQSPGAVAFSYEMLSDTRIFFTD
                     GRPHVNNNIKMYLGDSRAHWDGEDLVVETTNLTDRTAFGVNGNGPLHSTKMKVTERFR
                     RVAADILQYQITVDDPETYVRPFTVSMPLTPLEGGKLLPYDCHPGNYAILQSLGAERV
                     EDQRLADDLRRGIIRPRVGVQEGAGVGAAPAGGGGGGGRGRGAGPAF"
     gene            complement(1508..2470)
                     /gene="glsA"
                     /locus_tag="AC16CY_07435"
     CDS             complement(1508..2470)
                     /gene="glsA"
                     /locus_tag="AC16CY_07435"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HIR3353654.1"
                     /translation="MSRHALQHAIEQLHARNRSSTDGHVATYIPELGLANPDDFGISL
                     VTAAGEAFETGDHFRPFTIQSISKPFTFGLALEELGEEEVSRRVGVEPSGDAFNSIEL
                     QSGTNRPHNPMVNAGAIAVTALLHHRHTENTFEVILDRLSRAAGRRLSVDERVFESER
                     RSGHRNRAIAHLLLNFGLVHDEAEHALDVYFRQCSILVTCRDLAMIGATLANMGLNPV
                     TGQPVFDVLTAKHMLSIMFTCGMYDYSGEWAYRVGVPAKSGVSGGVLAIVNRQMAIAT
                     YSPRLDARGNSCRGIDVCVDLAARFGLHAFDCVNVGSSFLESVI"
     gene            2707..>3604
                     /locus_tag="AC16CY_07440"
     CDS             2707..>3604
                     /locus_tag="AC16CY_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353655.1"
                     /translation="MSDDINDCRRLLFIARSDAGAFRGGPLGAREKVATQLSELLPRL
                     TFDDAGMGTFTRGTAELTVQLVSDPVSAIEMTLRPEHMEAFRPPLERVATRTGWQLLD
                     ADRSEVIFPLPTAPAAAPSGGSSVLRWALVALLTVGGVAGGAWWTFVGSTEYLLVPAP
                     SRMAGSGGAAPRASMPSLDAPGGIPELAGFPEGVPRSVEEFAAQAQQIGEIVARQRRM
                     APEFRGMRVVNEMMMVDQAEVQFQASVGNGLYVDPAELTDHSSLPPDFGIMTLPPQFA
                     QPSRGGYRFSFSGENKGKPFSDA"
BASE COUNT          614 a         1154 c         1197 g          639 t
ORIGIN      
        1 ccgacaggcg tgggctcaac tgaaacagag cattggtgat tcttgatctt tgattggtga
       61 ttcgttgttg attggtgatc tctgattgtc gatttatcga tgatcgactg gcacaacgat
      121 cacggacaaa tcacgaatca agaatcaaca gtcgtcaacg aatcaacaat tcaaagatca
      181 acaatctaca acgtgcggcc gcctagaagg cggggcctgc tccccggccg cggccaccac
      241 cgccgccccc gcccgccggc gcggcgccga ctccggcgcc ttcctgcacg ccgacgcgcg
      301 gacggatgat gccgcgcctg aggtcatcgg ccagtctctg gtcttcgacg cgctcggcgc
      361 caagcgactg aaggatggcg tagttccccg ggtggcagtc gtacgggagc agcttgccgc
      421 cttcgagcgg cgtcagcggc atggagactg tgaaggggcg cacgtacgtc tccgggtcgt
      481 ccaccgtgat ctgatactgc aggatatcag cggccacgcg ccggaaccgc tcggtgacct
      541 tcatcttggt gctgtgcagg ggaccgttgc cgttcacgcc gaacgccgtg cgatcggtca
      601 ggttggtcgt ttccacaacg aggtcctcgc cgtcccagtg cgcgcgggag tcgcccagat
      661 acatcttgat gttgttgttg acgtggggcc ggccgtcggt gaagaagatg cgcgtgtcgg
      721 acagcatctc gtacgaaaac gcgaccgcgc ccggcgactg cacgatgcgg ttgccgttgc
      781 cgtagatcac gttcagcgcc gaaccgaaga tgccgcgggt gatgcagcgt tcgtagcccg
      841 agaagtcggt cacccagtcg agcggacctg gcccgaacgt gcctctcggc atctgacgcc
      901 tgccaggcag cgttgccggc atctggccgt tcgggggatc gaccacctgg gacgtctgcg
      961 ggaaggcgcg gcgcgcgaag tcgaaccgga acgtgctgtc ggcctcgttc tccgaccgcg
     1021 cgacgccgct ctcgatctgt ttcttgcgcg ctgcaagctc ctgctcgttc aggaacaggc
     1081 gatcgccgta ctgcgccggc ctcgacatcg ggatgccggc catgtcgtcg ctcgcccaga
     1141 cgccctgcag atcgggatcg ccccatggcg tccgcggcgc cttgtaagtg atgggagccc
     1201 cgccgaaggc cgttactacg agcgccggtg gcgccacgct cgcctgtgtc gcctgggcag
     1261 tctggccgga caccgggacc gacatcaagg cgccaaccgc cgtacacgcc gcgagcgtga
     1321 ctgccgaacc gatcattcgc gtggtcatcg tcgactcctt gcctcgatcc cgagggggcg
     1381 gaaacgaagt gctgagactg cccacgattc ttgggccgtg gagtcaggct gtcaagaggc
     1441 acgcgagcct ccgggcagac aatcctgtcg gattggcaaa ttgcgttgca gaaatggctg
     1501 aatccgtcta gatcaccgac tccaggaaac tggaaccgac gttcacgcag tcgaacgcgt
     1561 ggaggccgaa tcgcgccgcc agatccacgc acacgtctat cccgcggcag ctgttgccgc
     1621 gcgcatcgag ccgcggcgag taggtggcga tcgccatctg tcggttgacg atcgccagca
     1681 caccgccgct gaccccgctc ttggccggga cccccacccg ataggcccac tcacccgagt
     1741 agtcgtacat cccgcacgtg aacatgatcg acagcatgtg cttcgccgtg aggacgtcga
     1801 acaccggctg gccggtgacc ggattcagtc ccatattggc cagagtggca ccgatcatgg
     1861 ccagatctcg gcacgtgacc aggatcgagc actgccggaa atagacatcg agcgcgtgct
     1921 cggcctcgtc gtggaccagc ccgaagttga gcagcaggtg cgcgatggcg cgatttcgat
     1981 ggcccgagcg ccgctccgat tcgaatacgc gctcgtcaac cgacagcctc cggcccgcag
     2041 cccggctcag tcgatcgagg atcacctcga acgtgttctc cgtgtggcga tggtggagaa
     2101 gcgcggtcac ggcgatcgcg ccggcgttca ccatcggatt gtgtggtcgg ttggtgccgc
     2161 tctggagctc gatcgagttg aaggcgtcac cgctcggctc gacgccgacc cggcgcgaca
     2221 cctcctcttc tcccagctcc tcgagcgcca ggccaaacgt gaacggcttg gagatcgact
     2281 gaatcgtgaa cggacggaag tggtcgccgg tttcgaaggc ttccccggcc gcggtcacca
     2341 gtgaaatgcc gaagtcatcg ggattggcca atccgagctc cgggatgtag gtggcaacgt
     2401 gcccgtcggt cgacgaacga ttacgggcgt ggagttgctc gattgcgtgc tggagtgcgt
     2461 gtctcgacat gaggtggggg ctccgatcgc gtccgggtgc gtctagcaac tgacgtgccg
     2521 catcaacttc tgaaaagcgc ggacgtcatc gctgttcgtg ccgcgtctgc cggacaagat
     2581 cgtccgttcg agctggtgag gcgacgagaa tgtcgggaac gccatcggcc ggtggtgacg
     2641 ccaaacgcgt cgcatgctgg ccccgtaatg ggagcgaccg acgccgccga caaccaaaag
     2701 ggacgtatgt ccgacgacat caacgattgc cgtcgtttgc tgttcattgc gcgctccgac
     2761 gcaggcgcgt tccggggcgg gccgctcgga gcgcgtgaaa aagtggcgac ccagctctcg
     2821 gagttgctgc cccggctcac gttcgacgac gcgggcatgg gtacgttcac gcgcggcacc
     2881 gcggagctga ccgtgcagct cgtgagcgac ccggtctcgg ccatcgagat gacgttgcga
     2941 cccgagcaca tggaggcgtt caggcctccg ctcgaacgcg tcgcgacgcg gacagggtgg
     3001 cagctcctcg acgcggatcg cagcgaggtc atcttcccgc tccccaccgc gccagcggcg
     3061 gcaccttccg gcggttcgtc ggtcctgcga tgggcgctcg tcgcgctgct caccgtcggc
     3121 ggcgtcgcgg gcggtgcgtg gtggaccttc gtgggatcca ccgagtactt gctcgtgccg
     3181 gcgccgtcgc gaatggcagg ctccggcgga gccgcgccgc gcgcctcgat gccgtcactc
     3241 gacgcgcctg gcggcatccc ggagctggca gggtttccgg aaggcgttcc gcggtcggtc
     3301 gaggaattcg cggcgcaggc gcagcagatc ggcgagatcg tggcgcggca gcgccggatg
     3361 gccccggagt tccgcggcat gcgcgtcgtc aacgagatga tgatggtcga tcaggcggag
     3421 gtacagttcc aggcgtcggt cggcaatggg ctctatgtcg atccggcgga gctgaccgac
     3481 cactcgagtc tgccacccga tttcggcatc atgacgctgc cgccgcagtt cgcgcagccg
     3541 tcgcgcggcg gatatcgctt ctcgttttcg ggagagaaca aggggaagcc gttctccgat
     3601 gcgt
//
LOCUS       DBNGSH010000317         2348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3421297, whole genome shotgun sequence.
ACCESSION   DBNGSH010000317 DBNGSH010000000
VERSION     DBNGSH010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3421297"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /locus_tag="AC16CY_07445"
     CDS             <1..2127
                     /locus_tag="AC16CY_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3353656.1"
                     /translation="GTVEYMAPEQIEGQEADARTDIFALGVVVYEMLTGRRAFSGRTH
                     ASLIGAILKDDPPPLSRDQPLAPPFLDHIVRRCLAKDPDERWQTAADLMRELSFAAAH
                     ARDRGPDASVAGQPVARTRRMIPATYAVALAVAVAAATATGAWLLRLQSPRAEPGGLR
                     LSIALAPGAEIGDVDNPAIAISPDGRRVVYAAVGAGGRQLFLRSIDSFDAQALPGTED
                     ASNPFFSPDGAWVGFFSKGKLRKLSIAAGAAQELSDAPAPRGGSWSPDGVIYFAPRVS
                     SGLFKVSSEGGSVTEVTKLDRSKGEVSHRFPHVLPGGAGLMFTVWTGPGTDEKQIDVL
                     NLDTGERHSVAQGAETAVHASSGHIVYGRADTLMAAPFDLAGLTVGGAAVRVAEGVRV
                     GHGEGGQFTMSSTGDLLYLPTDRRRSERRLVWVDRQGQVEALQVPTRNFANAKVSPDG
                     RFAAVNLNGSVTEIGIVDLSRSTVTLLTTGKGSSQAPVWSPDGMRIAYRATRSGYRNL
                     FGKGVNDTADEERLATSDNVQTPSSWSADGKWLFFNETSQASDNDVFAMAIDDDRKVE
                     ALIKTSNNLQMSRDGRWLAYQSTEQGRSEVFVQPFPPGGQRVQISNGGGNEPLWAPDG
                     RELFYMRGDRMMVVDITTRPTFQAGAPRLLFEGRFVPSPNGVTGYSISPDARRFLMVQ
                     AVQPEPPATVLHLVRGWAEELTRLAP"
BASE COUNT          383 a          779 c          808 g          378 t
ORIGIN      
        1 ggcacggtgg agtacatggc ccccgagcag atcgagggac aggaggccga cgcgcgaacc
       61 gatatcttcg cgctcggggt cgtcgtctac gagatgctga cggggaggcg cgcgttcagc
      121 ggccggacgc acgcgagcct gattggcgcc attctcaagg acgatccgcc gccgctctcg
      181 cgcgatcagc cgcttgcgcc gccgtttctc gaccacatcg tcagacgatg tcttgcgaag
      241 gacccggacg agcggtggca gacggcggcc gatctcatgc gcgagctgag ctttgccgcg
      301 gcacacgcgc gcgaccgcgg gccggacgcg tccgttgcgg ggcagccggt ggcgcgcacg
      361 aggcggatga ttccggcgac atacgcggtc gcgttggccg tcgccgttgc cgctgcaacc
      421 gcgacgggcg catggctgct tcgactccag tcgcctcgcg ccgaacccgg cgggttgcgt
      481 ctgtcgattg cgctcgcacc cggggccgag atcggcgacg tggacaatcc cgcgatcgcg
      541 atatcgccgg acggtcgccg cgtcgtgtat gcggcggtcg gcgcaggcgg ccggcagctc
      601 tttctgcggt cgatcgacag cttcgacgca caggcgctgc ccgggaccga ggatgcgtcc
      661 aatccgttct tctctcccga tggcgcctgg gtcggcttct tctcgaaggg caagctcagg
      721 aagctgtcca tcgccgctgg agctgcgcag gaactgtccg atgctccagc cccccgcggc
      781 gggagctgga gccccgatgg cgtcatctac tttgcgccgc gcgtctcgtc cggactgttc
      841 aaagtctctt cggaaggcgg cagcgtcacg gaggtgacga agctcgatcg ctcgaagggg
      901 gaagtgagcc accggttccc ccacgtcctg ccaggcggag ccggcctcat gttcaccgtg
      961 tggaccggtc ccggcaccga tgaaaagcag atcgacgtcc tcaatctcga cacgggcgag
     1021 cgtcactccg tcgcgcaggg cgccgagacc gccgttcatg cgtcatccgg tcacatcgtc
     1081 tacggccgtg ccgacacgct gatggctgca ccgttcgacc tggccggctt gaccgtcggc
     1141 ggcgccgcgg tgcgcgtggc cgagggcgtt cgcgtcgggc acggcgaggg aggacagttc
     1201 acgatgtcct cgacaggaga cctgctgtat ctgcccaccg atcgccgccg ctccgagcga
     1261 cggctggtgt gggtcgatcg tcagggccag gtcgaggccc tgcaggttcc cacgcggaac
     1321 ttcgcgaacg ccaaagtctc cccggacggc cgcttcgcgg ccgtcaacct caacggctcg
     1381 gtgaccgaga ttggcatcgt cgacctttcg cgatcgacgg tcacgctcct caccacgggc
     1441 aagggcagca gccaggcgcc ggtgtggtcg ccggatggaa tgcgaatcgc ctatcgagcg
     1501 acacgcagcg ggtatcgcaa cctgttcggg aagggggtca acgatacggc cgacgaggag
     1561 cgtctggcaa caagtgacaa cgtgcaaacc ccgagctcgt ggtcggccga cggcaaatgg
     1621 ctgttcttca acgagaccag ccaggccagc gacaacgacg tgttcgcgat ggcaatcgac
     1681 gacgaccgca aggtcgaggc gttgatcaag acgtcgaaca acctgcagat gtcacgagac
     1741 ggccgctggc tggcgtatca gtcgaccgag caggggcgct ccgaggtgtt cgttcagccc
     1801 ttccccccag gtgggcagag ggttcagatc tcgaacggcg gaggaaacga gccgctgtgg
     1861 gcgccggacg ggcgcgagct gttttacatg cgaggcgaca ggatgatggt ggtggacatc
     1921 acgacgcgcc ccacgtttca agctggcgcg ccccgcctgc tgttcgaagg ccgcttcgtg
     1981 ccgtcaccca acggcgtgac gggctactcg atctcgcccg acgcccggcg cttcctgatg
     2041 gtgcaggccg tgcagcccga gccgccggcg accgtgctgc acctggttcg aggctgggcc
     2101 gaggagctga cgcggctcgc gccgtaacgt ccgcgcgggc cgaggagctg acgcggctcg
     2161 cgccgttaac gaccgcgcgg gctgaagccc gcgctctaca atgtctggat cccaacgccg
     2221 tatgacacgg tactccgccg tatgacacgg tactccaacg ccgtagagcg gaggcttcag
     2281 cctccgcgga gacagcgtat gacgcggtac attccggcgt tgctcgtggc gatcgccgcg
     2341 atcgcttc
//
LOCUS       DBNGSH010000318         3097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1383661, whole genome shotgun sequence.
ACCESSION   DBNGSH010000318 DBNGSH010000000
VERSION     DBNGSH010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1383661"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /gene="pal"
                     /locus_tag="AC16CY_07450"
     CDS             <1..489
                     /gene="pal"
                     /locus_tag="AC16CY_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR3353657.1"
                     /translation="QPAPATTETSTPDATTATTTPQRVDDPLPVPQQPLGEDSIASKP
                     LDDLNRESPLKPVFFALDSADLDAAGRAVTTANADILKRFATWVVTVEGHCDERGTPE
                     YNLALGERRAAAVKAYLTSLGIPAARIRTVSYGKEFPFNPGHTEEAWAQNRRGHLVIT
                     SQ"
     gene            500..1399
                     /locus_tag="AC16CY_07455"
     CDS             500..1399
                     /locus_tag="AC16CY_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3353658.1"
                     /translation="MQKTIIAGAAWLALLIAAPAAAQNREHQQLSAELRQLQEQNQQL
                     SLAIAQLTEALKAVNVRLDANEQFQQRRFADQENLVKNVGTDLSAIRERSQDIDRRMR
                     SLSDEVDALSKTILSLPALIAQAQPQPPAAPALDPNNPNPAVPPPIDKPPAVSAAPTI
                     SLPPTAGLSPTRLYDTAFSDYGGGQYTAAIAGFEQVLKYFPDAEKADDAQQLIGDSLS
                     HLKRFQEAIAAYNLVIQKYPNGDQVEIAYYHRGTAESLLGRTELARATWEELVKRYPK
                     SREADLARQRLNGLSRPNPTPQK"
     gene            1499..1927
                     /locus_tag="AC16CY_07460"
     CDS             1499..1927
                     /locus_tag="AC16CY_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3353659.1"
                     /translation="MGSVNKVILVGNLGRDAELRYTPGGAAVATLNLATTEVWNDRNN
                     QRQEKTEWHRVVLWGKPAESLQEYLTKGKQIYVEGRLQTRQWDDKDGNKRYTTEIKAD
                     RVTLLGGGGRGGGMDRGAPHPAASPDEPPAEPITDDDIPF"
     gene            complement(1924..2268)
                     /locus_tag="AC16CY_07465"
     CDS             complement(1924..2268)
                     /locus_tag="AC16CY_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3353660.1"
                     /translation="MTRVEKPWGYELHWAKTDRYVGKILHINAGHALSLQYHNQKDET
                     IYLYSGKLLYEIEIDGLLTKREMKPGDSIHVPPKTVHRMTAIDDCDVFEVSTPELDDV
                     VRIQDRYGREGT"
     gene            complement(2265..>3097)
                     /locus_tag="AC16CY_07470"
     CDS             complement(2265..>3097)
                     /locus_tag="AC16CY_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR3353661.1"
                     /translation="DEQYHVYRQLVDRMAGRPVTIRTFDIDESQLARPLVDAALDARW
                     FPDEQRSGHAGLRGIRFGLAQPRIFKTQLRALLRAAAHGPLRIMFPFVSSAQEVRAAR
                     ALLLEARHELGTRVPGDLTVPVGIMIEVPSAAFMASLLAKEADFFTIGTNDLIQYTLA
                     VDRTDDRVSNRYEPLHPAVLRLLRQVRRAAAREKIPVSVCGEMASDPLSLKLLIGCGL
                     REFSMTPGAIAIARSVVRETSARDMVRVAAKALTLGSVEEIERFVSEAVGAYEGRSAR
                     "
BASE COUNT          581 a         1052 c         1016 g          448 t
ORIGIN      
        1 cagcctgcac ccgccacgac ggagacgtct acgcccgacg cgacgacggc aacgaccact
       61 cctcagcgcg tcgacgatcc gctccccgtg ccgcagcagc cgctgggcga agactcgatc
      121 gcaagcaagc ccctcgacga cctgaaccgg gagtcccctc tcaagccggt gttcttcgcg
      181 ctcgacagcg ccgacctcga cgccgccggc cgggccgtca cgacggcaaa cgccgacatc
      241 ctcaaacgtt tcgcgacgtg ggtggtcacc gtcgagggac actgtgacga gcgcggcacg
      301 ccggaataca atctggcgct cggtgaacgc cgggccgcgg ccgtcaaggc gtacctgacg
      361 tcgctgggca tcccggcggc gcggattcgc acggtcagct acggcaagga gtttccgttc
      421 aatccagggc acaccgagga ggcgtgggcg cagaaccgcc gcggccacct cgtgatcacg
      481 tcccagtaag gaccgaggaa tgcagaagac catcatcgcc ggagcggcct ggctggcgct
      541 gctcatcgcg gcgcccgctg ccgcgcagaa ccgcgagcac cagcagttgt cggcggagct
      601 ccggcagctg caggagcaga atcagcagct cagtctggcg atcgcacagc tgacggaggc
      661 gctcaaggcc gtcaacgtgc ggctggacgc caacgagcag ttccagcagc ggcggttcgc
      721 ggaccaggag aatctcgtca agaacgtcgg cactgatctc agcgcgatac gggaacgatc
      781 ccaggacatc gacaggcgca tgcggagcct ctccgacgaa gtcgatgcgc tcagcaagac
      841 gatcctgtct ctgcctgccc tgatcgcgca ggcgcagccg cagccgccag cggcgccggc
      901 cctggatccg aacaacccga acccggccgt cccacccccg atcgacaagc cgcctgcggt
      961 atccgcggcg ccgaccatct cgctgccgcc gaccgccggg ttgtcgccca cacggttgta
     1021 cgatacggcg ttcagtgatt acgggggggg acagtacaca gcggccatcg ccggattcga
     1081 gcaagtactc aaatactttc ccgatgcgga gaaggccgac gacgcgcagc agctgatcgg
     1141 ggattcgttg tcacacctga aacgcttcca ggaggccatc gccgcataca atctcgtgat
     1201 tcagaaatac ccgaacgggg atcaggtcga gatcgcgtac taccatcgag ggacggctga
     1261 gagcctgctc ggacgcaccg agctggcgcg ggccacgtgg gaagagctcg tcaagcggta
     1321 ccccaagagc cgcgaggccg accttgcgag gcagcgcctg aacggtctga gccgccccaa
     1381 cccgacaccc cagaaatagt gacctgggct gtcacgctgt ccgggtagac tcgctactgg
     1441 cacggccgca gcgactgcag ccgtaggggc ggctcggccg cccgaaagga atcgatacat
     1501 gggcagcgtg aacaaagtga tcctcgttgg caacctcggg cgcgacgcag aacttcgcta
     1561 cacaccgggc ggcgccgccg tcgcaacgct caatctcgcc accacagaag tctggaacga
     1621 cagaaacaat caacggcagg aaaagaccga atggcaccgc gtcgtcctgt ggggaaagcc
     1681 ggccgagagc ctgcaggagt acctcacgaa gggcaagcag atctacgtcg aggggcggct
     1741 gcagacgcgc cagtgggacg acaaggacgg caacaagcgc tatacgaccg agatcaaggc
     1801 tgaccgcgtc acgctgctcg gcggcggcgg caggggcggc ggcatggacc gcggcgcgcc
     1861 gcacccggcg gccagccccg acgaaccgcc ggcggaaccg atcaccgacg acgatattcc
     1921 gttctaggtg ccctctcggc cgtaccgatc ctggatccgc acgacgtcgt cgagctccgg
     1981 agtcgagact tcgaaaacgt cgcagtcgtc gatggccgtc atccggtgca cggtcttcgg
     2041 cggcacgtga atcgagtcgc cgggcttcat ctcccgtttg gtgaggagcc cgtcgatctc
     2101 gatctcgtac aggagcttgc ccgagtagag gtaaatcgtc tcgtctttct ggttgtggta
     2161 ctgcaggctc aaggcgtggc cagcgttgat gtgcaggatc ttgccgacgt agcggtccgt
     2221 cttcgcccag tgaagctcgt agccccaggg cttctcgact cgggtcacct cgcgctcctt
     2281 ccttcgtagg ccccgacggc ctcggagacg aagcgctcga tctcctcgac cgagccgagc
     2341 gtcagcgcct tggccgccac gcgcaccatg tctcgggcgc tcgtctcgcg caccacgctg
     2401 cgggcgatcg cgatcgcgcc tggcgtcatg ctgaactcgc gaaggccgca tccgatgagc
     2461 agcttcagcg acagcggatc ggacgccatc tctccgcaca ccgagacggg aatcttctcc
     2521 cgcgcggcgg cgcggcgaac ctggcgcagc aggcggagca cggcgggatg cagcggttcg
     2581 taccggttcg agacgcggtc gtcggtgcgg tccacggcaa gcgtgtactg aatcaggtcg
     2641 ttggtgccga tcgtgaagaa gtcggcctcc ttcgcaagga gcgacgccat gaaggcggcc
     2701 gacggcactt cgatcatgat gccgacaggg acggtgaggt cgcccggcac tcgagtcccg
     2761 agctcgtggc gagcctcgag gagcagcgcc ctggccgccc tgacctcctg ggccgacgag
     2821 acgaacggaa acatgatccg cagcggcccg tgcgccgccg cgcggagcag cgcccgaagc
     2881 tgcgtcttga agatgcgggg ctgcgcgagg ccgaaccgga tgccgcggag cccggcatgg
     2941 ccgctccgct gctcgtccgg gaaccatcgc gcgtcgagcg ccgcgtccac gagcggccgc
     3001 gcgagctggc tctcgtcgat gtcgaaggtc cggatcgtga ccggcctgcc cgccatgcgg
     3061 tctacgagct gccggtacac gtggtactgc tcgtcct
//
LOCUS       DBNGSH010000319         4896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1723807, whole genome shotgun sequence.
ACCESSION   DBNGSH010000319 DBNGSH010000000
VERSION     DBNGSH010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1723807"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="AC16CY_07475"
     CDS             <1..401
                     /locus_tag="AC16CY_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353662.1"
                     /translation="EAIKVLLSAGANPNAKSGDGTPLLHQAVQARQVPIIRALAGAGA
                     KLDATNKDNITALQLAEKPEPPPPPGNNTDSRAYRPKRDSREDVIAALRELMGLGPND
                     PTPVPPPVETKKPEEKKADPAPESEDQQGA"
     gene            401..3010
                     /locus_tag="AC16CY_07480"
     CDS             401..3010
                     /locus_tag="AC16CY_07480"
                     /inference="COORDINATES: protein motif:HMM:NF019259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3353663.1"
                     /translation="MKNVLSSAALVWACAALLWAQTPVRLKPDTTSAAPTQKAETASA
                     TPTQKPGTTTAAPATVRPPSTALGPGKPDTTSTSAAPAQAAKHQAWVKQHCVGCHNTR
                     SPQPANNPVNLETASLEDLLPHAATWERVLRKLSVRAMPPPPLPHPPEAEYVAFTNWL
                     AASLDRAWQGKSTPGRYVVHRLNRTEYGNAIRDLLALDVDVTELLPSDGGNFGFDNIA
                     SSLGTSPLLFERYLAVAQRISVMAVGDATARPGATEYPISREFTQTGHIEGLPLGTRG
                     GAVVRHVFPADAEYKLFGRLVRGVEEGYAGVEGNETPDTFIITIDGEQVFSAQVGGLK
                     DHAVQVKDMNEAKALVDARMAGRVFVTAGPHDVGFTWRERPSERQDVWQPALRDSQEV
                     HMIGGHTRLKVVGIEGPYDVKGISSSPSREKIFVCQPTSPANERACAEKIFTNLTRRA
                     YRRPTTAADVEPPMTFYTQARDSGGNFDAGIRAGVARVLASPSFLYRIERDPAGARVG
                     TAHAVSDIELASRLSFFLWSSIPDEALMNLATAGRLREPGVLAAQVRRMIADPRADAL
                     FSNFVGQWLQLRNLESKVQPDLLMFPDFDDNTRKAFRRETELFFGHMLRENRSALELL
                     NADYTFVNERLARHYGIPGVYGSRFRQVKIADPNRRGLLGHGSILSLTSVATRTSPVF
                     RGKYILTTFLNTPPPPPLPNVPTLEESNKEATAPKTVRAQLELHRNNPTCASCHRIID
                     PAGFALENFNSVGQWRDRGEGGAAIDTAGVLADGSKVDGPVALRNVLLSRPDAFVTTL
                     TERIMTYALGRGVEPSDMPVIRGIVKRAAAENYRFSSIIMGVVESAPFQMRTKLEPAD
                     TTNRVAQAASVQK"
     gene            3082..4419
                     /locus_tag="AC16CY_07485"
     CDS             3082..4419
                     /locus_tag="AC16CY_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3353664.1"
                     /translation="MIITKKHLSRRTFLQGTFGAAMALPMLDAMVPALTAQSRTAART
                     PFRFGAIYMPNGVYPDTWHPSTLGSGFEFKPVMQPLEPFRDQLVTVSKMKAPWGESVH
                     LGASSAFLNGVGPIGSKGAGGDAFGKLESKKTLDQFIADTVADDTPLRSIEVGTEDMG
                     TAVGACDGFPCTFFNALSWRDDVSPLPIGINPRVTFERMFGETGSTEQRTARLKEKQS
                     LLDSVLQETAKLRGTLGAPDRAILDEYLSNIRQVEQQLDRMETRLGTITGTPEAPLGL
                     PEAFDDHMAVTYDLMHLAFQGDISRVFTFMIGHEASDRGYAHIGIPETHHSISHHGND
                     AEKLAKYAKLCTYQIVKFGEFLDKLKSTKDADGTLLDHSLLYWGSGMSNGNAHDRSNP
                     PAVLVGGAHGRLKGDRHIEAKKDEPTANLLLAMAQLAGAEIESLGASTGKLDL"
     gene            complement(4416..>4896)
                     /locus_tag="AC16CY_07490"
     CDS             complement(4416..>4896)
                     /locus_tag="AC16CY_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353665.1"
                     /translation="VPIGAAHLLLLVIPLHAAARIVRAPSHAPSTTAVLAYMQSVFSV
                     LFTSGVALMWTGLTLGDPDFGRRLHEAVTEGLPIDAMLARMSAIMETAVSAPFLAAFA
                     LTNVIWLYSAGWLLVASDVFSQLWRISRVRATAILVAVALVLVLESGLVALAWAVGR"
BASE COUNT          897 a         1681 c         1652 g          666 t
ORIGIN      
        1 tcgaggccat caaggtgctc ctctccgcgg gggcgaaccc gaacgccaag tcgggcgacg
       61 gcacgccgct gctgcaccag gcggtgcagg cgcggcaggt gccgatcatt cgcgcgctgg
      121 cgggagcggg cgccaagctc gacgcgacca acaaggacaa catcacggcg ctgcaactgg
      181 ccgaaaagcc cgagcctccg ccgccgcccg gcaacaacac cgattcgcgc gcgtatcgtc
      241 cgaagcggga ttcgcgcgag gacgtcattg cggcgctgcg cgagttgatg ggtctcggcc
      301 ccaacgatcc aacgcccgtg cctccgccgg tggagaccaa gaagcccgag gagaagaaag
      361 ctgatcccgc ccccgagagc gaggatcagc agggagcgtg atgaagaacg tattgtcgag
      421 cgcggcactc gtctgggcct gcgccgcgct gctgtgggcg caaacaccgg tccggctaaa
      481 gccggacact acgtctgccg cgcccacgca gaaggcggag acggcgtctg ccacgcccac
      541 gcagaagccg ggcacaacga ccgcggcgcc cgccacggtc cggccgccct cgactgcgct
      601 cgggccagga aagccggaca ccacgtcgac ttctgccgcg cccgcgcaag ccgcgaagca
      661 ccaggcctgg gtgaagcagc actgcgtggg atgccacaac acgcgatcgc cgcagccggc
      721 gaacaacccc gtgaacctgg aaacggccag cctcgaggat ctcctgcccc atgcggcgac
      781 gtgggagcgc gtcctgcgca agctgagcgt gcgggccatg ccgcctccac cgctgccgca
      841 tccgccggag gccgagtacg tcgcgttcac gaactggctg gccgcctcgc tcgaccgcgc
      901 ctggcagggc aagagcacgc cgggccggta cgtcgtgcac cggctgaacc gcactgagta
      961 cggtaacgcc atccgcgatc tgctcgcgct cgacgtggac gtcacggagc tgctgccgag
     1021 cgatggcggg aacttcgggt tcgacaatat cgcgtcctcg ctgggaacct cgccgctgct
     1081 gttcgagcgg tacctcgccg tcgcgcagcg catcagcgtg atggccgtcg gcgacgcgac
     1141 cgcgcggcct ggcgcgaccg agtatccgat cagccgcgag ttcacgcaga ccgggcacat
     1201 cgaaggcctg ccgctcggaa cgcgcggcgg cgccgtcgtg cgccacgtgt ttcccgccga
     1261 cgccgaatac aagctgttcg gccggctggt tcgcggcgtc gaggaaggct acgccggcgt
     1321 cgagggcaac gagacgcccg acaccttcat catcaccatc gacggcgagc aggtgttttc
     1381 cgcgcaggtc ggcggcctca aggatcacgc ggtccaggtc aaggacatga acgaggccaa
     1441 ggcgctggtg gacgcgcgca tggcgggccg cgtgttcgtc accgccggcc cgcacgacgt
     1501 gggcttcacc tggcgcgagc ggccgtccga gcggcaggac gtgtggcagc ccgcgctgcg
     1561 cgacagccag gaagtgcaca tgattggcgg ccacacgcgg ctcaaggtcg tgggcatcga
     1621 ggggccgtac gacgtgaagg gcatcagcag ctcaccgagc cgcgagaaga tcttcgtgtg
     1681 ccagcccacg tcgccggcga acgaacgagc ttgcgctgaa aagatcttca cgaacctgac
     1741 gcggcgcgcc taccgccgcc ccacgacggc cgcagacgtc gagccgccga tgacgttcta
     1801 cacgcaggcg cgcgacagcg gcggcaactt cgacgccggc atccgcgcgg gcgtggcgcg
     1861 cgtcctggcg agcccgtcct tcctctaccg gatcgaacgc gatccggccg gcgctcgcgt
     1921 gggaaccgcg cacgcggtca gcgacatcga gctcgcgtcg cgactgtcgt tcttcctctg
     1981 gagcagcatc ccggacgagg cgctgatgaa cctggcgacg gcgggccggc tgcgcgagcc
     2041 tggcgtgctc gccgcgcagg tgcgccgcat gatcgcggat ccgcgcgccg atgccctctt
     2101 cagcaacttc gtggggcaat ggctgcagct tcgcaacctg gagtcgaagg tccagccgga
     2161 cctgctgatg ttcccggact tcgacgacaa cacgcgcaag gccttccgcc gcgagaccga
     2221 gctgttcttt ggccacatgc tgcgcgagaa ccgcagcgcg ctcgagctgc tgaacgccga
     2281 ctacaccttc gtgaacgagc gtctcgccag gcactacggc atcccgggcg tctacggatc
     2341 gcgcttccgc caggtgaaga tcgcggatcc gaaccggcga ggcctgctcg gccacgggag
     2401 catcctgtcg ctgacctccg tggccacgag gacgtcgcct gtgttccgcg gcaagtacat
     2461 cctcacgacg ttcctgaaca cgccgcctcc gccgccgctg ccgaacgtgc cgacgctcga
     2521 agaaagcaac aaggaagcga ccgcgccgaa gacggtcagg gcgcagctgg aactgcatcg
     2581 caacaacccg acgtgcgcgt cctgccatcg catcatcgac ccggcgggct ttgcgctcga
     2641 aaacttcaac tcggtcggcc agtggcgcga caggggcgag gggggcgcgg ccatcgacac
     2701 cgcaggcgtg ctggcggacg gctcgaaggt ggacggcccg gtggcgctgc ggaacgtgct
     2761 cctgagcagg cccgacgcct tcgtgaccac gctgaccgag cggatcatga cgtatgcgct
     2821 cggccggggc gtcgagccct cggatatgcc cgtcattcga ggcatcgtaa aacgggcggc
     2881 agccgaaaat taccggtttt cctctatcat tatgggggtc gtcgaaagcg cgccgttcca
     2941 aatgcggacc aaactggagc cggccgatac gacgaacagg gttgcacagg cggcgagcgt
     3001 tcagaagtga ctgactgacg tagtgtccgg cttcagccgg accgacgtag tgtccggctt
     3061 tagccggacc gtggagcagc catgatcatc acgaagaagc atctgtcccg gcgcacgttc
     3121 ctgcagggta ccttcggcgc cgcgatggcg ctgcccatgc tcgatgcgat ggtgccggcg
     3181 ctcacggcgc agtcgcgcac ggcggcgcgc acgcccttcc ggttcggcgc gatctacatg
     3241 ccgaacggcg tgtacccgga cacctggcat ccctccacgc tcggcagcgg cttcgagttc
     3301 aagccggtca tgcagccgct cgagccgttc cgcgatcagc tcgtcacggt cagcaagatg
     3361 aaggcgccgt ggggcgagtc cgtgcacctg ggggccagct ccgcgttcct caacggcgtc
     3421 ggccccatcg gctcgaaggg cgccggcggc gacgccttcg gcaagctcga gtcgaagaag
     3481 acgctcgatc agttcatcgc cgacacggtg gccgacgaca cgccgctgcg ctcgatcgaa
     3541 gtgggcaccg aggacatggg caccgccgtg ggcgcgtgcg atggattccc gtgcacgttc
     3601 ttcaacgcgc tctcgtggcg cgacgacgtg agccccctgc cgatcggcat caacccccgg
     3661 gtgacgttcg agcggatgtt cggcgagacc ggctcgaccg agcagcgcac cgcgcggctg
     3721 aaggagaagc agagcctgct ggattcggtg ctgcaggaga cggcgaagct gcgcggcacg
     3781 ctcggcgcgc cggaccgcgc gattctcgac gagtacctga gcaacatccg ccaggtcgaa
     3841 cagcagctcg accggatgga gacgcggctt ggcacgatca cgggcacgcc ggaggcgccg
     3901 ctcggactgc ccgaggcgtt cgacgaccac atggccgtga cctacgacct gatgcacctg
     3961 gcgtttcagg gcgacatcag ccgggtgttc accttcatga tcggccacga ggcgagcgac
     4021 cgcggctacg cgcacatcgg catcccggaa acgcatcaca gcatctcgca ccacggcaac
     4081 gacgccgaga agctcgcgaa gtacgcgaag ctctgcacgt atcagatcgt caagttcggc
     4141 gagttcctcg acaagctgaa gtccacgaag gacgccgacg ggacgctgct cgaccactcg
     4201 ctgctctact ggggcagcgg catgagcaac ggcaacgccc acgatcggag caacccaccg
     4261 gccgtcctgg tcggcggagc gcacggccgg ctgaagggcg accgccacat cgaggccaag
     4321 aaggacgagc cgacggcgaa cctgctgctc gccatggcgc agttggcggg tgcggagatc
     4381 gagagcctcg gggccagcac gggaaaactc gacctctagc ggccgaccgc ccaggcaagc
     4441 gcgacaagtc cggactcgag gacgagcacc agcgccacgg cgaccaggat cgcggtggcg
     4501 cgcacgcggg agatccgcca caactggctg aagacgtcgc ttgcgaccag cagccagccg
     4561 gccgagtaca gccagatcac gttcgtaagc gcgaaggccg ccaggaacgg cgcggagacg
     4621 gctgtctcca tgatggccga catccgggct aacatcgcgt cgatcggcag accttccgtc
     4681 accgcctcgt gcaggcgccg gccgaaatcg ggatcgccga gcgtcagtcc agtccacatc
     4741 agcgcgacgc ctgacgtgaa gagcactgaa aacaccgact gcatgtaggc gagcacggcc
     4801 gtcgtcgatg gtgcgtgact cggcgcgcgc acgatccggg cggctgcgtg aagcggaatc
     4861 acgaggagca gcagatgcgc agctccgatg ggaacc
//
LOCUS       DBNGSH010000320         2089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1213880, whole genome shotgun sequence.
ACCESSION   DBNGSH010000320 DBNGSH010000000
VERSION     DBNGSH010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1213880"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="AC16CY_07495"
     CDS             complement(<1..139)
                     /locus_tag="AC16CY_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR3353666.1"
                     /translation="MFTGLIEAVGEVSEITPTSSGYRLRIHMPEQLGLEPGDSVAVNG
                     VC"
     gene            complement(120..1202)
                     /gene="ribD"
                     /locus_tag="AC16CY_07500"
     CDS             complement(120..1202)
                     /gene="ribD"
                     /locus_tag="AC16CY_07500"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR3353667.1"
                     /translation="MVDDYRWMRRALEHARRGLGRTTPNPVVGACVVSDEGVVVGQGA
                     HERAGEPHAEVNALEEAGSLARGATLYCTLEPCAHTGKTGPCTDRIIAAGIRRVVAAM
                     EDPFPLVGGRGFATLRAHGIEVETGIGYEDAVRLNQPFLVTTRKGRPFVIMKAATSLD
                     GRIAAAAGRRTQLTSTRALTHAQYMRAQVDAIVIGSETVLVDDPRLTAREVYRERPLT
                     RVVLDRRLRTPPAARLLSTLPAGPVIILTTPDAARSARAGALRTRGVSVLPVAREGMT
                     AALSVLAGLDVQSVVLEGGAQVQRAAWDEGIVDYVQLYLAPVWLGEEGVPLLEGCTFS
                     PMALLERRVEQLGPDVLIEGYVHRPH"
     gene            complement(1196..>2089)
                     /gene="ftsY"
                     /locus_tag="AC16CY_07505"
     CDS             complement(1196..>2089)
                     /gene="ftsY"
                     /locus_tag="AC16CY_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3353668.1"
                     /translation="ARTTQQIVGRFEAIVQQADAGEHRTRPIDVDTIEALEELLISAD
                     VGLPATEAVVTAVKSRARHGESLRDLVKEEIRKIFDRVEAPAPPDGHHPHVTLIVGVN
                     GTGKTTTIGKLANLLKASGKTPLVCAADTFRAAAVEQLEIWASRAGVDIVRANEGSDP
                     ASVVFDAIQSGKARGRDPILIDTAGRLHTRVNLMNELDKIRRVAARAVEGAPHEVLLV
                     LDATVGQNGLQQAREFTSVAGVTGIVLTKLDGTAKGGVAVSIANDLKLPIRYVGIGEG
                     IDDLIPFSAQDYVDALFQDKW"
BASE COUNT          303 a          763 c          684 g          339 t
ORIGIN      
        1 ggcacacgcc gttgacggcc acgctgtctc ccggctccag ccccagctgc tctggcatgt
       61 ggattcgcag ccggtacccg gacgacgtgg gcgtgatttc agagacttcg ccgacggctt
      121 caatgaggcc ggtgaacata accttcgatc agcacgtccg gtccaagctg ctcaactcgc
      181 cgctcgagga gcgccatcgg cgagaacgtg cagccctcga gcagcgggac gccttcttca
      241 ccgagccaga ccggcgcgag atacaactgg acgtagtcca cgatgccttc atcccaggcc
      301 gcgcgctgca cctgcgctcc gccttcgagc acgacgctct gcacgtcgag gccggctagc
      361 accgagagcg ccgcggtcat gccttcccgg gccaccggga gcacgctgac gcccctggtc
      421 ctgagcgccc cggcgcgcgc cgacctcgcg gcgtcgggcg ttgtcagtat tatcaccggt
      481 cccgccggga gggtcgacag cagccgggcg gccggcggcg tccgcagacg gcgatccagc
      541 acgactcgcg tcaggggcct ctcccggtac acctcccgcg cggtcagacg agggtcgtcc
      601 acgagcaccg tctccgagcc gatcacgatg gcatccacct gggctctcat gtactgggcg
      661 tgcgtcagcg cccgcgtcga cgtgagttgg gtccggcggc ccgcggcggc ggcaatccgt
      721 ccgtcgaggc tcgtcgccgc cttcatgatc acgaacggcc gccccttgcg cgtcgtcacc
      781 aggaacggct ggttcagccg gaccgcgtcc tcgtagccga tgccggtctc cacctcgatg
      841 ccatgcgcgc gcagcgtcgc gaatccgcgg cctcccacga gcggaaacgg atcctccatg
      901 gccgccacca cccgacggat gccggccgcg atgatccgat cggtgcacgg gccggtctta
      961 ccagtatgtg cgcagggttc gagcgtgcag tacagcgttg cgccgcgtgc gaggcttccc
     1021 gcttcctcta gcgcattgac ctcggcgtgc ggttcgcccg cgcgttcgtg ggcgccctgc
     1081 cccacgacca cgccttcgtc ggagacgacg cacgcaccca cgacgggatt gggcgtcgtg
     1141 cgtccgaggc cgcgccgcgc atgttcgagg gcacgccgca tccaccgata gtcgtctacc
     1201 acttgtcctg gaacaacgcg tccacgtagt cctgcgccga gaacgggatc aggtcgtcga
     1261 tgccctcgcc gatcccgacg taccggatgg ggagcttcaa gtcgttggca atcgacacgg
     1321 cgacgccgcc cttggccgtg ccgtccagct tcgtcagcac gatgccggtg acgccggcca
     1381 cgctcgtgaa ctcgcgcgcc tgctggagcc cgttctgccc gacagtcgcg tcgagcacga
     1441 gcagcacttc gtgaggcgcg ccttccaccg cccgcgccgc gacgcggcgg atcttgtcca
     1501 gctcgttcat caggttcacg cgcgtgtgga gccggccggc cgtgtcgatg aggatcggat
     1561 cgcgcccgcg agccttcccc gactggatcg cgtcgaagac cacagacgcc gggtcggacc
     1621 cttcgttcgc gcgcacgatg tccacgcccg cgcgcgaggc ccagatctcg agctgctcga
     1681 cggccgccgc gcgaaacgtg tccgcggcgc agaccagcgg cgtcttgccg gacgccttga
     1741 gcaggttcgc cagcttgcca atcgtggtcg tcttgcccgt gccgttgacg ccgacgatca
     1801 gcgtcacgtg cggatgatgc ccgtcaggag gagccggcgc ctcgacgcgg tcgaagatct
     1861 tgcggatctc ctccttgacc aggtcccgaa ggctctcgcc atggcgcgcc cgcgacttga
     1921 cggccgtcac gacggcctcg gtcgcgggga gaccgacgtc ggcggagatc agcagctctt
     1981 cgagcgcctc gattgtgtcg acgtcgatgg gccgtgtgcg atgttcgccg gcgtcggcct
     2041 gctggacgat cgcctcgaag cggccgacga tctgctgcgt cgtccgcgc
//
LOCUS       DBNGSH010000321        11738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3591647, whole genome shotgun sequence.
ACCESSION   DBNGSH010000321 DBNGSH010000000
VERSION     DBNGSH010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3591647"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC16CY_07510"
     CDS             <1..219
                     /locus_tag="AC16CY_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353669.1"
                     /translation="LRLYAENMGWTVVIEMGGEIEPLVHAAAEHAIQLVLCWRLSDMP
                     EAESLLTRLHTTGVDLLPLAQSCAALQE"
     gene            522..722
                     /locus_tag="AC16CY_07515"
     CDS             522..722
                     /locus_tag="AC16CY_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3353670.1"
                     /translation="MSTNGTIKRLTDKGFGFIATPEGTEYFFHQSACTSKPFDSLREG
                     ESVTFTIGQGPKGPRAENVVPA"
     gene            876..1772
                     /locus_tag="AC16CY_07520"
     CDS             876..1772
                     /locus_tag="AC16CY_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353671.1"
                     /translation="MRSSVVSGVVAALVLAAGCESTRSQAFRPARTADGKPDLNGIWQ
                     ALNTANFDIQGHASRPALALMPAPARSGVPGLVRATRVELPHPSVRSLGAVGGVPAGE
                     SVVEGGEIPYQPWAAAKKRENAEHWLERDPEIKCYMPGVPRATYLPYPFQILQGTNKI
                     VMAYEFAGATRTIHLDSVPDSPAPTWMGWSRGRWEGDSLVVEVTDFNEETWFDRAGNF
                     HSDELHVTERYTPTSADHLLYEATIEDPKVFTRPWKIRMPLYRRLEPGKKLLEYKCVE
                     FVEELMYGQLGVVDPATGQKNK"
     gene            1789..2796
                     /locus_tag="AC16CY_07525"
     CDS             1789..2796
                     /locus_tag="AC16CY_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353672.1"
                     /translation="MNARSPVLTLALAAVVGAAIATIGAQQDTKPRTTPWGDPDLQGV
                     WRYEAAIPLERPARFEGRESLTDEELKESQRIEDEQARQRLAGAEGAAVGRRSVAESP
                     IRGNEYNSFWQDHGRPRQVLRQTSLIVDPRDGRLPYTADARKADARASARYGVGPYES
                     YLDPDTGERCLTDGVTALMWQGPNGGHNRIVQSPGYVTILHEEYLDRRIIPTDGRSHG
                     SIRQWFGDAVGKWEGSTFIVDTINFIDRTNYEWASIFTRPSETLHIVERFTRTGADAM
                     EYRITVDDPTTFERAWTAVIPITRLSDETQIYEYACHEGNYAMTNLLAGGRADAARRS
                     R"
     gene            2800..3228
                     /locus_tag="AC16CY_07530"
     CDS             2800..3228
                     /locus_tag="AC16CY_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3353673.1"
                     /translation="MRSHLMFLFVVSAGSLPASLHAHHAFAAEFDASKPFKIRGTVVK
                     MEWINPHTWLHLDVKRPDGKTERWMIEGGPPNALYRRGFTKNSLPQGAELVVEGFRAK
                     DGTLKGNGRELTFADGRRLFVGSSGTGAPNDGREPQGGKE"
     gene            complement(3240..6872)
                     /locus_tag="AC16CY_07535"
     CDS             complement(3240..6872)
                     /locus_tag="AC16CY_07535"
                     /inference="COORDINATES: protein motif:HMM:NF052691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7948 domain-containing protein"
                     /protein_id="HIR3353674.1"
                     /translation="MDHDGTDADFLARGSGYLVFLDRGAATLLMDETADRQRVSIAFR
                     LMGASPLATGRGRHILPGVTNYLIGNDPRRWRTGLRSFAEVEYRDVYPGVDLVYYGNQ
                     RRLEFDFVVAPGADYRAIGLTFSGSTRVSIDAQGNLVLATDSGNLVQHAPTIYQVDAG
                     TRRSVRGGYVIHEGGRVGFRVGNHDPHLPLIIDPVLTYSSYLGGAREERAAGVAVDAS
                     GHMYVAGLTRAADFPISAPPGLTHGRDNWDAFVVKLTPNGDQFEYATYVGGGGYDEPA
                     SLAVDDAGNAYVTGLTESSDFPMLNALQSSRRGFRDGFVAKLDANGSIAYSTHLGGNG
                     IDSGTGIAVDALGRAYVTGETSSADFPTANAAQRSLSGSPAFRTTDGSATWAGISNGL
                     NTSHVTMFAIDPVEPAIVYAGTNADGVFVSTDAGSTWSATSASLPPARVNGMVVDSGG
                     VLYVASNAGVFRSADHGTSWTNLQFFAGAWTIAVDPSGVVYVAFSAGEFPTLGLFKST
                     NGGTTWNLAGLPIGVMSLAVSQSVVYAGTVQGLFKSDNGSTWTPVAIDSQQPAPIITA
                     LSVDPHNPDLVYAGTSAGLFVSTSGGATWAPVSVFAGAHVMNVAIAPSNPDIVYAATS
                     FGGGLTEDGGVTWRGAPPVGINVPFFAVDPLDPTRAYAAGGVGLDVFVSRVSPDGSTL
                     QYSTFLGGIGSESGSDIAVDDTGAAYVTGTTQLGGFPVRNAYQATPGGFMDVDVFAAK
                     FSAAGAVVYATYLGGSQSDYRSKIAVDSAGRAHIVGLTLSTNFPTANAYQPLHGGGFS
                     DVFVTTLNPAGNGLAFSTFLGGSDQEVDGTQILGPDVALGPFGETFVTGATRSTNFPR
                     REAIQATYGGGAADAFVAAFDAAGQLQSSTYLGGSGQDTGRRVAFDFTGGALVVAGST
                     NSTNFPTRQPLQAQNAGDADVFIARMTVDAPPPADTIAPTTAIGVSGTAGSNGWFRSN
                     VTITLSASDNQGGSGVSYVEYNLDNWPFQRYSAPFSIAGQGATVVRARATDEAGNTEN
                     PVSTVVAIDTAPPAISVSSPTSADYLHSAALQLSFAATDATSGIDGSPTARLDGTSVT
                     SGETVQLLAMTLGSHTLVVTSSDRAGSTSTQTVTFRVIATIDSLIAAVNTFAAQGSIA
                     SNTAQRLIAKLEDAKRALNRGNVTAARGDLNEFSDHVSAQQGKSIAASAAQVLLDDVA
                     FVRAGI"
     gene            7374..8384
                     /locus_tag="AC16CY_07540"
     CDS             7374..8384
                     /locus_tag="AC16CY_07540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3353675.1"
                     /translation="MTTHDVRRLFQRLLALAVAIAAIGVVVAAQQPAPPADPAAARER
                     QAEINKRPDTPGTGRFPAMKEEVSSLPRHVVYRPSDLAGLGPTKLGVVAWGNGGCSDD
                     GASSRFHLLEIASHGYLVIASGRILSGPDAPAREARPAATPGQPQPPRTQVSDLTDAV
                     DWAVTENQRSGSPYFGRIDPAMIAYSGWSCGGVQALQVAKDPRVKTLVIHNSGVLNNG
                     PTNMTGISVGKEVLQTLHTPVIYIEGGPTDIAYANGMDDFNRVTHVPIAMANLAVGHG
                     GTFNEPNGGAAASVAVSWLDWQLRGDAQGARRFVGEDCGLCKDTQWTLQRKQFPAAPG
                     SR"
     gene            8381..9154
                     /locus_tag="AC16CY_07545"
     CDS             8381..9154
                     /locus_tag="AC16CY_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3353676.1"
                     /translation="MTESRYPGGSPRFATTHWSIVVGAGSRDTAQARESLSRLCQAYW
                     FPLYAYVRRRGHAPDDAQDLTQAFFARLLEKNWVAGANRSKGKFRTFLLSAMKHFLAD
                     EWDRARAQKRGGGATHLTLSLDTAEERYAVEPSDPATPESIFERRWALALLDVVLDRL
                     RAEHEADGRGALFAALRPCLVGERTAQPYAELAQSLGATEASIKSAVHRLRQRYRQLL
                     RDEIASTLDAPEDVDDELRHLFAVLSAPGAGPQPFPGAS"
     gene            9174..10709
                     /locus_tag="AC16CY_07550"
     CDS             9174..10709
                     /locus_tag="AC16CY_07550"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3353677.1"
                     /translation="MADNTCPSCGKTLPAGAPQGLCPECLIRSGFNTGTAPDGSATAR
                     GFTPPAVEELRPLFPQLEILELIGRGGMGAVYKARQPALDRLVALKILPSTIAGDPGF
                     ADRFNREARALARLQHPRIVMVHDFGIAGGLHYLLMEYVDGPNLRAVQRSGRLAPAQA
                     LRIVPEICEALQFAHDRGIVHRDIKPENLLLDREGHIKITDFGIAKMVGPDAETAALT
                     GTVGVVGTPHYMAPEQIERPADVDHRADIYSLGVVFYELLTGELPIGRFAIPSSRVQL
                     DVRLDEVILRSLEKEPARRYQQVSQVKSRLDTIAQSPGKAPAVARSGRIIAIPAMILY
                     VLQALLAVPIPVLWLRMLSPMGAQFPVAAGPGFSPLGYWAPFAWLWFFIFALLGAYLA
                     WGVLHYQCWTALPERYRATTPAQAAGFIFIPLFNFYWVFVSLPGLADGFNAYREEHPG
                     SPIRNARGLGVAKAIGFVCGWTIGWIPGVGSIVAIVDAILFVLYYRAIVSNANQVIAA
                     DAK"
     gene            10758..>11738
                     /locus_tag="AC16CY_07555"
     CDS             10758..>11738
                     /locus_tag="AC16CY_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353678.1"
                     /translation="MKRCAASVVCACSVLMGLASSAGAQQPATRGIVNVTGQLYRAQN
                     NNHYTVFLVTPEGVIMSDPINRDFARWLKGEIATRFKQPVRYVLYTHRDWDHASGGVV
                     FADTAEFVGHRNMLTALAPPAGNPPLTPDAVKMDANRNGLVERSEATGAIAERFDLFD
                     YNRDGIVTGAEVARGSVSDVYPPTVTYTDRHTVTLGGKRVTMAYLGVAHADDSSVLHF
                     PDERAVFSADIMQVKRLPGGLAPTVGSWIDALSIITALDFEHALTGHALSGTKKDAMQ
                     SLQYLIDISKGVAAGVGAGRSLAEIQKTLTLDAYKGFERWDTIREAHIAAV"
BASE COUNT         2095 a         3899 c         3840 g         1904 t
ORIGIN      
        1 ctccggctct atgccgagaa catgggctgg acagtcgtga tcgagatggg cggagagatc
       61 gagccgctcg ttcacgccgc cgccgaacac gccatccagc tggttctctg ctggcgactg
      121 agcgacatgc ccgaggccga atcgctgttg acacgtctcc acacaaccgg cgtcgatctc
      181 ttgccgctcg cccagagctg cgccgccctg caggagtaag gtggatcaat ccgggaggac
      241 cagacgctcg atattgctgg gaaaaaccac gtaggacaac cggtagccca cacgcctggg
      301 taacaacgac ctttcgggac ttagggagca ttcgctgtag acttgggcga gcccgcgaca
      361 tcagtcccaa gaccatctca acgacacgac attcacctac aaagctcacg ctggggaacc
      421 gggccacggc cctgctgcct cgatgcgcga cgggtgtggg acgaagaccc acgcggtaag
      481 tcaggcggcc aattccggcc agttcttcag aggattcaca catgtctacc aacggcacca
      541 tcaagcgcct cacggacaaa ggcttcggct tcatcgccac gcctgagggc accgaatact
      601 tcttccacca gtccgcgtgc acgagcaagc cattcgactc cctgcgcgag ggcgagagcg
      661 tgacgttcac catcggccag ggcccgaaag gcccgcgcgc cgagaacgtc gttccggcct
      721 gagcactcct cgacaaccga accccggtgg cctgacagga catcggggtt cgacctcgtg
      781 cgtctgcggc tcgacgttcg gtcggcttgc tcatgaccga taatacggcg ctgcgagtga
      841 tcccgtaatc cgcccttgcg gcaaggagga tccggatgcg gtcgagcgtc gtgagcggtg
      901 tcgtggcggc cctcgtcctg gcggccggct gcgagtccac gcgcagccag gcctttcggc
      961 cggcgcgcac ggccgacgga aagccggatc tgaacggtat ctggcaggcg ctcaacaccg
     1021 cgaacttcga catccagggc cacgcctcca ggccggctct ggcgctgatg ccggcgccag
     1081 cgcgcagcgg agtgccaggg ctcgtgcgag cgacccgcgt ggaacttccc cacccgtcgg
     1141 tgcggtctct cggggccgtg ggcggtgtgc ccgccggcga gagcgtcgtc gagggcggcg
     1201 agatcccgta ccagccgtgg gccgctgcga agaaaaggga gaacgccgag cactggctgg
     1261 agcgcgaccc cgagatcaag tgctacatgc cgggggtgcc gcgagcgact tacctgccgt
     1321 acccgttcca gattctgcag ggcaccaaca agatcgtgat ggcgtacgag tttgccggcg
     1381 cgacgcgcac cattcacctc gacagcgtcc ccgacagccc ggcgccgacg tggatgggat
     1441 ggtcgcgcgg ccgctgggaa ggggactcgc tcgttgtcga ggtgacggac ttcaacgagg
     1501 agacgtggtt cgaccgggcc ggcaacttcc acagcgacga gctgcacgtg accgaacgct
     1561 acacgccaac gagcgccgat cacctgctgt atgaggccac gatcgaggac ccgaaagttt
     1621 tcacgcgtcc atggaagatc cgcatgccgc tgtaccggcg actcgagccg gggaagaagc
     1681 ttctcgagta caaatgcgtc gagttcgtcg aagaattgat gtacggccag ctcggcgtcg
     1741 tcgacccggc gaccggacag aagaacaagt agacggaagg gctgccacat gaacgctcgc
     1801 tctccagtcc tgacgcttgc cctcgccgcc gtcgttggcg cggccatagc gacgatcgga
     1861 gcacagcaag acacgaagcc gaggacgaca ccgtggggcg atcccgatct gcagggtgtc
     1921 tggcggtacg aggccgccat cccgctcgag cggccggcgc gcttcgaggg gcgcgagtcg
     1981 ctcacggacg aggaactcaa agagagccag cggatcgagg acgaacaggc caggcagcgc
     2041 ttggccgggg cggagggcgc ggcggtggga cggcgatcgg tcgccgagtc gccgattcgc
     2101 gggaacgagt acaacagctt ctggcaggac cacggacggc cgcggcaggt cctccggcag
     2161 acatctctca ttgtcgatcc cagggatggc cggctgccgt atacggcgga tgcccggaaa
     2221 gccgacgcgc gagccagtgc gcgctacggc gtcgggccct acgagtccta cctcgacccc
     2281 gataccggag agcgctgtct cactgacggc gtcacggcac tgatgtggca aggccccaac
     2341 ggcggacaca atcgaatcgt gcagagcccc ggctacgtca ccatcctcca cgaggagtat
     2401 ctcgaccggc gcatcatccc gaccgacggc cgcagccacg gcagcatccg gcagtggttc
     2461 ggcgacgctg tcggcaaatg ggaaggctcg accttcatcg tcgatacgat caacttcatc
     2521 gatcgtacga actacgagtg ggcgtcgatc ttcacgcgtc cgtcggagac gctgcacatc
     2581 gtcgagcgct tcactcgaac gggtgcggac gccatggagt accgcatcac cgtggacgat
     2641 ccgacgacgt tcgagagggc gtggacggcg gtgattccga tcacgcggct gagcgacgag
     2701 acgcagatct acgagtacgc ctgccacgag ggcaactacg cgatgaccaa cctgctggcc
     2761 ggcgggcgtg ccgatgcggc caggcgatca cggtgaacca tgcgcagcca cctgatgttc
     2821 ctgttcgtcg tgagcgctgg gtcgttgccc gcgtcgctcc acgcgcacca cgccttcgcg
     2881 gcggagttcg acgccagcaa gccgttcaag attcgcggca ccgtcgtcaa gatggagtgg
     2941 atcaacccgc acacgtggct gcacctggac gtgaagcggc ccgacggcaa gaccgagcga
     3001 tggatgatcg agggcggccc gcccaacgcg ctctaccgcc gcggcttcac gaagaactct
     3061 ctcccgcagg gcgccgagct cgtcgtcgag gggttcaggg ccaaggacgg gaccctcaag
     3121 ggcaacggcc gggagctgac gttcgcggac ggccggcgcc tgttcgtcgg atcgtcgggc
     3181 accggcgcgc ccaacgacgg cagggaaccg cagggcggca aggaatagcg gcgaggacct
     3241 cagataccag cgcgcacgaa cgccacgtcg tcgagcagga cctgcgccgc actcgcagca
     3301 atcgattttc cctgctgcgc cgacacgtgg tcgctgaact cgttgagatc gcctcgcgcc
     3361 gctgtcacgt tcccccgatt caacgcccgc ttggcgtctt ccagcttcgc gatcaggcgc
     3421 tgtgcggtgt tcgaggcgat gctgccctgt gccgcgaacg tgttcaccgc cgcgatcagg
     3481 ctatcgatgg tcgcgatcac gcggaaggtc acggtctgcg ttgacgtgct gccggcgcgg
     3541 tccgaagagg tgaccaccag cgtgtgcgat ccgagtgtca tcgccagcag ttgaaccgtc
     3601 tcgccgctgg tgaccgatgt gccgtcgagc cgcgccgtgg gagacccatc gattcctgac
     3661 gttgcgtcgg tcgcggcgaa cgagagctgc agcgccgcac tgtggaggta gtcagcggat
     3721 gtgggtgagc tgacgctgat cgcgggcggc gccgtgtcga tcgcgacgac ggtggacacg
     3781 gggttctcgg tattgccggc ctcgtccgtc gcgcgtgcgc gaacgacggt tgcgccctgc
     3841 cccgcgatcg agaacggcgc gctgtatcgc tggaacggcc agttgtcgag gttgtactcg
     3901 acgtaagaca cgccgctgcc accctgattg tccgaggcac tcagcgtgat cgtcacgttc
     3961 gatcggaacc atccgttcga ccccgcggtt ccagatacgc cgatggccgt cgtaggcgca
     4021 atcgtatcgg caggtggcgg cgcatcgacc gtcatccgtg cgatgaacac gtcagcgtca
     4081 ccggcattct gtgcctgcag cggctggcgc gtcgggaagt tcgtggaatt ggtcgacccc
     4141 gccacgacga gagcgccgcc ggtgaaatcg aacgccacgc gacgaccggt atcctggccg
     4201 ctgcctccca ggtacgtgga cgactgaagc tggcctgcgg cgtcgaatgc cgccacgaat
     4261 gcgtcggcgg ctcccccgcc gtacgtcgcc tggatggctt cacgtctggg aaagttggtc
     4321 gagcgggtcg caccggtcac gaacgtttcg ccgaagggtc cgagtgccac atccggcccg
     4381 aggatctggg ttccgtcgac ctcctgatcg ctgccgccca ggaatgtcga gaacgcgagt
     4441 ccattgccgg ctggattcag cgtcgtcacg aacacgtcgg agaatccgcc tccgtgcaga
     4501 ggctggtacg cgttcgcggt cgggaagttc gtcgagagcg tgaggcccac gatgtgggct
     4561 cgtccggccg aatcgacggc gatcttcgac ctgtagtcgg attgggaacc tcccagatac
     4621 gtggcgtata cgacggcgcc ggccgccgag aatttcgcgg caaacacgtc cacatccatg
     4681 aagccgcccg gggtcgcctg gtaggcgttc cgtactggaa agccgcccaa ttgcgtggtt
     4741 cccgtgacgt aggcggcacc ggtatcatcg acggcgatgt cgctgcccga ttcggagccg
     4801 atgccaccaa ggaatgtgga gtactgcagc gtggatccgt cagggctgac tcgcgacacg
     4861 aagacatcga ggccgacacc gcctgcggcg tacgcgcgtg tcgggtcgag cggatcgacg
     4921 gcgaaaaacg gcacattgat cccgacaggc ggcgcccctc gccacgttac cccgccatcc
     4981 tcggtcagcc cgcccccaaa cgatgtcgcc gcgtacacga tgtccgggtt ggaaggtgcg
     5041 atggcgacgt tcatcacatg tgcgcccgca aagacagaca caggcgccca ggtcgcgcca
     5101 cccgatgtcg acacgaacag tccggcggaa gtgcctgcgt agaccagatc gggattatga
     5161 gggtcgacgg acagcgcagt gatgattgga gcaggctgct gggaatcgat tgccacgggt
     5221 gtccaggtac tgccgttgtc gctcttgaac agtccctgca ccgtgcccgc gtagacgacg
     5281 gactggctga cggcaagact catgacgccg atggggaggc cggcaaggtt ccacgtcgtt
     5341 cccccattgg tgctcttgaa caagccaagg gtcgggaatt cgccggccga gaacgccacg
     5401 tagacaactc ccgatgggtc gacggcgatc gtccacgcgc ccgcgaagaa ctgcaggttg
     5461 gtccagctgg tgccgtggtc tgcgctgcga aacaccccgg cattgcttgc gacgtacaac
     5521 actcccccgg aatcgacgac cattccattc acacgagccg gcgggagact ggcgcttgtg
     5581 gcggaccacg tggagcccgc atcggtgctg acgaagactc cgtccgcgtt ggtgccggca
     5641 tagacgatcg ccggctccac aggatcgatc gcgaacatcg tgacgtggct cgtgttcagc
     5701 ccgttcgaga tccccgccca cgtggcactg ccgtcggtgg ttcgaaacgc ggggctgccg
     5761 ctgagtgatc gttgggcggc gttcgccgtt ggaaaatccg ccgaggacgt ctcacccgtg
     5821 acgtaggcgc gcccaagcgc gtccaccgcg atgcccgtac ccgaatctat cccattgcct
     5881 ccgaggtgcg tggaataggc gatcgaaccg ttcgcatcca gcttcgcgac gaagccgtcc
     5941 ctgaacccgc gccgtgacga ttgcagggcg ttgagcattg ggaaatcgct cgattcggtc
     6001 aggccggtga cgtacgcgtt tccggcgtca tcgacagcca ggctcgcagg ctcgtcgtaa
     6061 ccgcctccac cgacgtaagt cgcgtactcg aactggtcac cattaggggt cagcttgacg
     6121 acaaaggcat cccagttgtc gcgtccgtgc gtgagcccgg gcggggcaga aatcgggaaa
     6181 tccgctgccc tggtcagacc cgccacgtac atgtgtcccg aggcatcgac cgccacgccg
     6241 gcggcacgct cctcgcgcgc acctcccaga tagctcgaat aggtcaacac gggatcgatg
     6301 atcagcggga ggtgcggatc gtggttcccg actcgaaagc caacccggcc gccttcgtga
     6361 atgacgtacc caccgcgaac cgatcgacga gtgcccgcat cgacctgata gatcgtcggc
     6421 gcgtgctgca cgagattgcc cgaatcggtg gcgagaacga gattaccctg ggcatcgatt
     6481 gacacacgtg tggatccgct gaacgtcagc ccgatcgccc gatagtccgc gcccggcgca
     6541 acgacaaagt cgaattcgag tcggcgctgg ttgccgtagt acacaaggtc gacccccggg
     6601 tagacatcgc ggtattcgac ctcggcgaag ctgcgaagcc ccgtgcgcca ccgccgcgga
     6661 tcgttgccaa tcaggtagtt cgtcacaccg ggcaggatgt gacgcccacg cccggtcgcg
     6721 agaggagagg cccccatcaa ccggaatgcg atcgagaccc gctgccgatc ggccgtctca
     6781 tccatgagca gcgttgccgc gcctcggtcg aggaacacga ggtaccctga gccgcgcgcc
     6841 aggaagtccg catcggtccc gtcgtgatcc accaaggcct cgaaccgcag aggtgtggcg
     6901 gcatagacct ctcgaacgcg accgggtctg tctgcctcac gttcgcctcc tgacgacagg
     6961 atggatgtcg tcgctgtcga agcgaagcgg gcgtcaattg agtctgttgc gaggcatggg
     7021 acaattgcgg caacgacgag cgccgtcgtg atgaagcagg cacgcgttcg catgatggtc
     7081 agactttctg gatcggaggg gttcgcgaac agagagcaac agcgaagcca accgtgcgcg
     7141 gccacagccc aggcaggaag gcaggcgcgg gcctggacca ccacacacca gcccttacag
     7201 aatcaggcgt ccactcactc agttcgtaat cggtgatctc gcggtgccgg gcggcggccg
     7261 gtgcctctcc gcttcgtggt cgcgtccacg aagcggcagg ccatcagcag cagatcttga
     7321 acggctcacg ggttacgagg acaatcggcg gcacactgcc aaggagcctc acgatgacca
     7381 cgcacgatgt ccgccgtctg ttccagcgcc tgctcgctct cgccgtcgcc attgcggcca
     7441 tcggtgtcgt cgttgccgcg cagcagccag cgccgccggc ggaccccgcg gccgcgcgcg
     7501 agcgccaggc cgagatcaac aagcggcccg acacgcccgg cacagggcgc ttcccggcga
     7561 tgaaagaaga agtgtcctcg ctgcctcgtc acgtcgtgta ccgcccgtca gacctcgccg
     7621 gtcttggccc cacgaagctg ggcgtggtgg catggggcaa cggcggctgc tccgacgatg
     7681 gcgcgagcag ccggtttcac ctgctggaaa tcgcctcgca cggatatctc gtgatcgcga
     7741 gcggccgcat cctgagcggc cctgacgctc cggcgcgaga ggcccgtccc gcggccacgc
     7801 ccggtcagcc acagccgccg cgcacacaag tgtcggatct caccgacgct gtcgactggg
     7861 cagtaacgga gaatcaacgc tcaggcagtc cgtacttcgg ccgcatcgat ccggcgatga
     7921 tcgcctactc gggctggagc tgcggcggcg ttcaggcgct ccaggtcgcg aaagatccgc
     7981 gtgtgaagac gctggtgatt cacaacagcg gcgtgttgaa caacggacca accaacatga
     8041 ccggcatcag cgtcggcaaa gaggtactgc agacactgca cacgccggtg atctacatcg
     8101 agggcgggcc caccgacatc gcatatgcca acggcatgga cgacttcaat cgtgtcacgc
     8161 acgtgccgat tgcgatggcc aatctggcag tcggacacgg cggcacgttc aacgaaccca
     8221 atggcggcgc cgccgcctcg gttgccgtga gctggctcga ctggcagctt cgtggcgacg
     8281 cgcagggcgc caggcgcttc gtcggcgagg actgcgggct ctgcaaggac acgcagtgga
     8341 cgctgcagcg caaacagttc ccggccgccc ccggcagcag gtgacggagt ctcgataccc
     8401 gggcggctcg cccaggtttg ccacgacaca ctggtcgatc gtcgtcggcg ccggcagccg
     8461 cgacacggcg caggcgcgcg aatcgctttc ccggttgtgc caggcctact ggtttccgct
     8521 gtatgcctac gtgcgccgtc gcggccacgc tcccgacgat gcccaggatc tgacgcaggc
     8581 gttcttcgcc cgcctgctgg agaagaactg ggtggccggc gcgaaccggt cgaagggaaa
     8641 gttccgaacg ttcctgctgt cggcgatgaa gcacttcctc gccgacgaat gggaccgagc
     8701 acgcgcccag aaacgcggcg gcggcgccac gcacctgact ctttcgctcg acaccgccga
     8761 agaacgatac gccgtggagc cgtccgatcc cgccacgccc gaaagcatct tcgagcggcg
     8821 atgggcgctg gcgctgctcg acgtggtgct cgatcgcctg cgggccgagc acgaggcgga
     8881 tggccgcggc gcgctgttcg ccgcgctgcg cccctgcctg gtcggtgagc gcacggcaca
     8941 gccctacgcg gagctggcgc agtcgctcgg tgccaccgag gcgtccatca agtccgcggt
     9001 tcaccgtctg cggcagcgct accgccagct tctgcgcgac gagattgcga gcacgctcga
     9061 cgcacccgaa gacgtggacg acgagctgcg tcatctcttc gccgtgctgt cggcacccgg
     9121 cgccggtccg caacctttcc ccggggcttc ctaagaagca ggtggaggac gccgtggccg
     9181 acaacacctg ccccagctgt ggaaagacac tccccgccgg agcaccccag ggactctgtc
     9241 ccgaatgcct gatcaggtcg ggtttcaaca ccggcaccgc tccggacggc tccgcaactg
     9301 cacgcggatt cacgccgccg gctgtcgagg agctccggcc gctgtttccg cagctcgaga
     9361 ttctcgagtt gatcggccgg ggcggcatgg gcgcggtgta caaggcacgg cagcccgcgc
     9421 tcgatcggct cgtggccctc aagatcctcc cgtcaaccat tgccggcgac ccgggcttcg
     9481 ccgaccggtt caatcgcgag gccagggcac tcgcccgtct ccagcatccg cgcatcgtca
     9541 tggtgcacga cttcgggatc gccggcgggc tgcactacct gctgatggaa tacgtggacg
     9601 gtccgaatct gcgcgcggtc cagcgaagcg gccggctcgc gccggcgcag gcgctgcgga
     9661 tcgttcccga gatctgcgaa gcgctccagt tcgcccacga tcgagggatc gttcatcgcg
     9721 acatcaagcc ggaaaacctg ctgctcgacc gcgagggaca tatcaagatc accgacttcg
     9781 gcatcgccaa gatggtcggg cccgacgccg agaccgccgc gctcacgggc acggtgggcg
     9841 tcgtcggcac gccgcactac atggcgcccg agcagatcga acgccccgcg gacgtggatc
     9901 atcgcgcgga catctattcg ctcggcgtcg tcttctacga gctgctcacg ggcgagctcc
     9961 cgatcggtcg cttcgccatc ccgtcgagcc gcgtgcagct ggacgtacgg ctggacgagg
    10021 tgatcctgcg ctcgctcgag aaggagcccg cgcgacgcta ccagcaggtc tcgcaggtga
    10081 agtcgcggct ggacacgatt gcccagtcgc cagggaaggc tccagccgtg gcgaggtcag
    10141 ggcgaatcat tgcgattccg gcgatgatcc tttatgtcct gcaggccctg ctggccgtgc
    10201 ccatcccagt tttgtggctg aggatgctct cgccgatggg agctcagttc ccagtggcgg
    10261 ccgggcctgg cttttcccct ttgggctact gggccccgtt cgcctggctg tggttcttca
    10321 tcttcgcgct cctcggcgcg tacctcgcgt ggggcgtgct ccactaccag tgctggacgg
    10381 cgctgcccga gcgctaccgc gcgacgacgc cagcgcaggc cgccgggttc atcttcattc
    10441 cgctcttcaa cttctactgg gtgttcgtct cgctgccggg gctcgcggat ggattcaacg
    10501 cctaccgcga ggagcatccg ggctcgccga ttcggaatgc gcggggcttg ggagtggcca
    10561 aggcgatcgg cttcgtctgc ggctggacga ttggctggat ccctggcgtc gggtcgatag
    10621 tggccatcgt ggacgcgatc ctgttcgtgc tgtattaccg cgcgatcgtt tccaacgcga
    10681 accaggttat cgccgccgac gcgaagtaag atctcctctt tgcgccgctg cgcgctccgc
    10741 gccggcagga ggtcatcatg aagcgatgtg cggcatcagt cgtctgtgcg tgctcggtgc
    10801 tcatgggtct cgcctcgagc gccggcgccc agcagccagc cacgcgaggc atcgtcaacg
    10861 tcaccgggca gctctatcgc gcgcagaaca acaaccacta caccgtgttc ctggtgacgc
    10921 cggaaggcgt catcatgagc gacccgatca accgcgactt cgcccgctgg ctgaagggcg
    10981 agattgcgac gcgcttcaag cagcccgttc gctacgtgct ctacacgcat cgcgactggg
    11041 atcacgcctc gggtggcgtc gtcttcgccg acacggcaga attcgtcggt catcgcaaca
    11101 tgctgacggc gctcgcgccg ccggccggca atccaccgct cacgcctgac gcggtgaaga
    11161 tggacgccaa tcgcaacggg ctggtcgagc ggagcgaagc caccggcgcc attgccgagc
    11221 gattcgacct gttcgactac aaccgcgacg gcatcgtcac cggcgccgag gtggcgcgag
    11281 gttcggtgag cgacgtgtac cccccgaccg tcacctacac ggaccggcac accgtcacgc
    11341 tcggtggcaa gcgggtcacg atggcatatc tcggcgtggc gcacgccgac gacagctcgg
    11401 tgctgcattt ccctgacgag cgtgccgtgt tcagcgccga catcatgcag gtgaaacggc
    11461 tgcccggcgg cctggcgccg accgtcggct cgtggattga cgcgctgagc atcatcaccg
    11521 ccctcgactt cgagcacgcg ctcaccggcc acgcgctctc gggcactaag aaggatgcga
    11581 tgcagtcgct gcagtacctg atcgacatct cgaagggtgt ggccgcgggc gtcggcgcgg
    11641 gccgcagcct ggcggagatc cagaagacgc tcacgctcga cgcctacaag gggttcgagc
    11701 gctgggacac gatccgcgag gcgcacattg ccgctgtc
//
LOCUS       DBNGSH010000322         9299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3591671, whole genome shotgun sequence.
ACCESSION   DBNGSH010000322 DBNGSH010000000
VERSION     DBNGSH010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3591671"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..2291
                     /locus_tag="AC16CY_07560"
     CDS             42..2291
                     /locus_tag="AC16CY_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3353679.1"
                     /translation="MTTPAPTESLSPDQSGQLAEFARTCRAAARSVSLYPKTHPSIGV
                     SLSRVVNAAGRLAKDGSVTLTVQRDMLTIGDRAPLRPDPSIGELASLLHDRLIGVLTI
                     RREADADDWHTFLVMLARPTEELMADGGVSSVWTRTGRTRFEIREIDYAEVLRERGGG
                     EAEWDRIVASCLKGETALDERALASLLDALGDPARFAALLNRIHTAAPDGIEVSARVA
                     ALLQLMKNAIEAAKARGEAEGERAVQTIAASCAGLTPDTMIAMLERRQSGQPDEAATA
                     SAVAGQMTHQTMASFMARSVTAERGATQRLAQALDALVPSAEQKERVVRLAKGEALQG
                     DLGADPDFDQIWQNVANMVMSYSMISYSDESWVSEQYGRELSAARRQAVEVERVSDDP
                     PERVQAWVATVTEPALLRLDLDMLLDLLRIEEVPDAWEPVAHIAASEAEHRTLAGDAS
                     SAQALIEAIVGDTKAGGRPALRACAARVVDRLCTGPLARHVGHQLRTIKDDEVERYAR
                     LCQALGPGLVRSLADALTREESNVAVRRLGVLLLGFGAAGRRSVEQLKNSSNPAVRRT
                     AVTLLRGAGGAEALSELASMLGDDDPAVQRESIHAIVEIGTDKAYAVLQRLLLEADTP
                     RDTALMELVNLRDDKAGPLFAYLLSHSRLRGKLAGFHLSMVEALGKMTPRAESVRALE
                     QVLHYRDWWALFRTTALQQAAANSLRRLGTPESVAVLEAAAAAGSRGVRRAARAQIAQ
                     LPGREGSQA"
     gene            2288..3640
                     /locus_tag="AC16CY_07565"
     CDS             2288..3640
                     /locus_tag="AC16CY_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3353680.1"
                     /translation="MNVQQRARLAEELSRRLAAAIRGINLYSPGHPLVRRNVIALLDS
                     IASLHEALPSVAVGIVGEDLVVGDIPLARAAETMGELMKRLRHLGIERIVIDRGVHER
                     EVEQLVETIAKAENRVQTEVLSSLPHIRVGRLDLEEQVTASQGDIATFRRLYDDAVKV
                     AGDLWESATSEGKPDADAAQEMVDSLAQAVAQNRTALLSLTALKNYDNYTFTHMVNVS
                     ILTMGQARGVGIDGSMLREFGMAALMHDIGKVKTPNEILNKPGKLTDDEYTILKKHTV
                     DGAEILRRTPEMPTLAPIVAFEHHLRADGTGYPAAVRPQLNLGTTLCGIADVYDAMRS
                     QRVYQAAHPTDRILAVLQRNDGKQFDQNLVRRFVQLVGIYPAGNLVRLDSGEVAVVLK
                     PHAPDPYRPQVRVLIDRDGQSMATPRDLNLWELHEGESRSILKPLDPEQYGIDPLVHL
                     "
     gene            3756..5369
                     /locus_tag="AC16CY_07570"
     CDS             3756..5369
                     /locus_tag="AC16CY_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3353681.1"
                     /translation="MRRTACLFGLLLALVIVISGLSAQSSPRLVVILAVDQLRADYLQ
                     TFNRHWQGGFRLLLDEGLNFDNARYPYLGTVTCAGHSTIGTGTQPRTHGMVSNTWWDR
                     ESRRMILCTGDPDTPDITYGRPVRSGNSAKWMLAPTLADELRAQRPGARVVALSLKSY
                     SAIGLAGHAGDAVVWFDDQAGSFATSRAYSPGPVPEVKAFIDGHPFEGDHGKVWTLSA
                     PASTYVMRDAGVGERPLPGWSGLFPHPLVGRSGPDSQFFDLWQSTPFADAYLERMAES
                     LIDSFALGQRDTTDFLGISFSVLDDVGHRFGPESREIEEVLRQLDATLKRLIAHLDAK
                     VGRLNYMLALTADHGVAPIAMAPRGGRIAGEDVRERIDETLAAHFGPLAQGSYVEATS
                     FSDVFLAPGLLDRLRQAPSTMLALEHALKEIPGITHVLRADQLSDRSPDPVVKTAALS
                     HIERRSGDLVLVPREYWYFAGRANATATTHGTLHEYDQHVPLIIFGGSARRGHDRSTA
                     SPADIAPTLARFAGIRMSKAEGRNLPGTW"
     gene            5381..5719
                     /locus_tag="AC16CY_07575"
     CDS             5381..5719
                     /locus_tag="AC16CY_07575"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR3353682.1"
                     /translation="MSFKQRVLAAVRRIPPGRVATYGDIAALAGSPRACRAVGTIMRD
                     CRDPATPCHRVISAGGLLGGFGGSPHLKRELLRAEALDVGVRRVRNFAAVRWQGGKKQ
                     ARGGRSGHLG"
     gene            5824..6372
                     /locus_tag="AC16CY_07580"
     CDS             5824..6372
                     /locus_tag="AC16CY_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3353683.1"
                     /translation="MRAVDLELAQRCQQGDSQAFEELYRAHAGRLYNLVFRMTASATE
                     AEDVLQEVFLHAHRKLGSFRGDSTLGTWLYRLTVNHCLDFLRGRQTRMTRVTESLDDE
                     RAAEPAAAAPVVPSAVSRIDLERAIGRLPEGCRAAFVLHDVQGFEHHEVARLLGVSEG
                     TSKSQVHKARMKLRGMLNETKS"
     gene            6369..7073
                     /locus_tag="AC16CY_07585"
     CDS             6369..7073
                     /locus_tag="AC16CY_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3353684.1"
                     /translation="MTCQDYEIELGDYVDGTLEGDARSAFEAHLSTCGRCTALVADFG
                     TILGAARVLEPEVPSPFLWTRISAAIESERGPGRWGFTWRTLASSAVAMMLVATMSWV
                     GAGLVPANEQGVRLAQAGPGGAEPISVAAQFELAEADYSSAIARLETITKAEQAALDT
                     DTAGVFRAQLTVIDGAVGESKAALATAPGDQVAQQSLFEALRSKLELLQDMLALINEM
                     RKGDPEGAARIVSGLN"
     gene            7073..8155
                     /locus_tag="AC16CY_07590"
     CDS             7073..8155
                     /locus_tag="AC16CY_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3353685.1"
                     /translation="MRLIQSSALVALVIAVLPPPAAAQAPSWVEELAKEIAENVERHA
                     EQIARFVEQRVEAQGRRGGRGGTEYTESFSRTVRLGRTGMFSLQNVSGDIVITGGGGD
                     DVRVDATKRVRHPNEAEAKSILSAVQIRVDEGSGRVDVRTDYPSRRNWSGSVDFQVTV
                     PRDASVNVRSVSGDVRVTNANGELRAETVSGDVIISSARRVGLVKSVSGDLQISDAEA
                     GELRFESVSGDVVVNKLKANGLDVNAVSGDVRISDVETGRATLRAVTGDVEFAGRLAR
                     NGRYQLQSHSGDVRMTPVDVRGFALEATTFSGEIRSDFAVTVQGTQGDPQSNRRVGRS
                     IRGSFGDGGTTLMLQSFSGDIILIRR"
     gene            8219..>9299
                     /locus_tag="AC16CY_07595"
     CDS             8219..>9299
                     /locus_tag="AC16CY_07595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HIR3353686.1"
                     /translation="MITSMPTASRVSPPPVPLPRRQEMERAYRGSDASYDGVFYLGVR
                     TTGIFCRPSCPARKPKPENVEFFALPKDALFAGYRPCLRCRPLDGDQAPPWVSRLLER
                     VDKRPTARIRERDLRVMGVEPARVRRYFSTRYGLTFQAYCRARRLALAFERIRNGGSL
                     DDAVFDTGYESHSGFRDAFRKAFGHPPGRSGDAGCIRLTWLDTPLGPMIGGATDAGVC
                     LLEFADRRMIENQLATLRRRFRAGLAPARHPHLAALERELGEYFAGTRRAFSVPTDTR
                     GTPFQTRVWRALNGIPYGETRSYRDIARQIGRPAAVRAVGHANGMNPVAIVVPCHRVV
                     NTSGALGGYGGGLWRKRRLLHLEAAP"
BASE COUNT         1512 a         3121 c         3224 g         1442 t
ORIGIN      
        1 gggcgcagta taactgccgc tgccttagaa tcagtttgtc catgacgacg ccggcgccga
       61 ccgagtcgct gtccccggac cagagcgggc agctggccga gttcgcccga acgtgcagag
      121 ccgcggctcg ctcggtgtcg ctctatccga agacgcatcc ctccatcggc gtgtccctct
      181 cgcgcgtcgt gaacgccgcg ggccggctgg cgaaggacgg ctcggtcacg ctgacggtgc
      241 agcgtgacat gctcacgatc ggtgaccgcg cgccgctgcg gccggacccg tcgatcgggg
      301 agctggcgtc gctgctgcac gacaggctga ttggcgtcct gacgatccgt cgggaggccg
      361 acgccgatga ctggcacacg ttcctcgtga tgctggcacg gccgacagag gagctgatgg
      421 cggacggcgg cgtgtccagc gtctggacgc gcacgggccg tacgcgcttc gagatccgcg
      481 aaatcgacta cgcagaggtg ctgcgcgagc ggggtggcgg cgaggccgaa tgggatcgca
      541 tcgtggccag ctgcctcaag ggcgagaccg cgctcgacga gcgcgcgctg gcatcgctgc
      601 tcgacgcgct cggcgatccg gcccgcttcg cggcgctgct caatcggatc cacacggctg
      661 cccccgatgg catcgaggtc agcgcgcgcg tcgcggccct gctgcagctg atgaaaaacg
      721 cgatcgaggc cgcgaaagcg cgcggcgagg ctgagggcga gcgggccgtg cagaccatcg
      781 cggcatcctg cgcgggcctc acaccggaca cgatgatcgc catgctcgag cggcggcagt
      841 cgggccagcc ggatgaggcg gcaacggcgt ctgccgtggc cgggcagatg acccaccaga
      901 ccatggcgtc gttcatggcg cgatcggtaa cggctgagcg gggcgcgacg caacggctcg
      961 cccaggcgct cgacgccctc gtgcccagcg ccgagcagaa ggagcgggtg gtgcggctgg
     1021 ccaaagggga ggcgcttcaa ggcgatctcg gcgccgaccc ggacttcgat cagatctggc
     1081 agaacgtcgc caacatggtg atgtcgtatt cgatgatttc gtactccgac gagtcgtggg
     1141 tgtcggaaca gtacgggcga gaactgtcag ccgcacggcg tcaggcggtg gaggtcgagc
     1201 gggtttcgga tgacccgccg gaacgggttc aggcgtgggt cgccacggtg accgagcccg
     1261 cactgctgcg gctcgatctc gacatgctgc tcgatctgct gcgcatcgag gaagtacccg
     1321 acgcgtggga gcctgtggcg cacatcgcgg cgtcggaggc cgagcaccgg actctcgccg
     1381 gagatgcctc gagcgcgcag gccctcatcg aggccatcgt cggcgacacc aaggcgggcg
     1441 gtcgtcctgc gctgcgcgcg tgtgccgcgc gcgtcgtcga ccgcctgtgc accggcccgc
     1501 tcgctcgcca cgtcggccac cagctccgga cgatcaaaga tgacgaggtc gagcggtacg
     1561 cgcggctctg ccaggcgctg ggccccgggc tcgtgcggtc gctggccgac gcgctgacgc
     1621 gggaggagag caacgtcgcc gtccggcgac tcggcgtgct gctgctcggc ttcggcgccg
     1681 caggccggcg gtcggtcgaa cagctcaaga actcgtcgaa ccccgccgtg cggcgcacgg
     1741 ccgtcacgct cctgcgcggc gccggcggcg cagaggcgtt gtcggagctg gcgtcgatgc
     1801 tcggcgacga cgacccggcg gtgcagcggg agtcgatcca cgctatcgtc gaaatcggga
     1861 ccgacaaggc gtacgccgtc ctgcaacggc tgctcctcga agcggatacg ccgcgcgaca
     1921 cggcgctcat ggagctcgtc aaccttcgcg acgacaaggc agggccgctc ttcgcgtacc
     1981 tcttgtcgca ttcgcgcctg cgaggcaaac tggcgggctt ccatttgtcg atggtcgaag
     2041 cgctggggaa gatgacgccg cgcgccgagt cggtccgtgc cctggagcag gtcctccact
     2101 accgggactg gtgggcgctc tttcggacga cagcgctcca gcaggccgcg gccaattccc
     2161 tgagacggct gggcacgccc gaatccgtgg cggttctcga ggcggcagcc gcggcaggaa
     2221 gccgcggtgt ccgaagggcg gctcgcgcgc agattgccca gctgcccggg cgggaaggga
     2281 gccaggcgtg aacgttcagc agcgtgcgcg gctggccgag gagctgtcgc gccgtctggc
     2341 ggcggcgatc cgcggtatca acctgtattc gcccggacac cccctcgtgc ggcgcaacgt
     2401 catcgccctg ctcgactcga tcgcgtcgct gcacgaggcg ctgccatcgg tggccgtcgg
     2461 catcgtgggc gaggacctcg tcgtcggcga catcccgctg gctcgcgcgg ccgagacgat
     2521 gggcgagctg atgaagcgtc tgcgccacct gggcatcgag cgcatcgtca tcgatcgagg
     2581 cgtgcacgaa cgcgaggtcg agcagttggt cgagacgatc gcgaaagccg aaaaccgcgt
     2641 gcagacggag gtgttgtcaa gtctcccgca catccgggtc ggccgtctcg acctcgaaga
     2701 gcaggtgacc gcgtcgcagg gcgacatcgc cacgttccgc aggctgtacg acgacgcggt
     2761 gaaggtggcg ggcgatctgt gggagagcgc cacgtcggag ggcaagcccg acgcggatgc
     2821 cgcgcaggag atggtggatt cactcgcgca ggcggtcgcg cagaaccgca cggcgctgct
     2881 ctcgctgacc gccctcaaga actacgacaa ctacaccttc acgcacatgg tgaacgtgtc
     2941 gatcctcacc atgggacagg cgcgcggcgt cggcatcgat ggatccatgc tgcgcgagtt
     3001 cggtatggcg gcgctcatgc acgacatcgg caaggtgaag acgccgaacg agatcctcaa
     3061 caagcccggc aagctgaccg acgacgagta cacgatcctg aagaagcaca ccgtcgacgg
     3121 cgccgagatc cttcgtagga cgccggagat gcccacgctg gcgccgatcg tcgccttcga
     3181 gcaccatctg cgcgccgacg gcactggcta tcccgcggcc gttcgtcctc agctcaacct
     3241 gggcacgacg ctctgcggga tcgctgacgt gtacgacgcg atgcgttccc aacgcgtcta
     3301 tcaggcagcc caccccaccg atcgcatcct ggcggtgctg cagagaaacg acggaaagca
     3361 gttcgaccag aacctggtcc gtcgcttcgt gcagctcgtg gggatctacc cggccggcaa
     3421 cctcgtgcgg ctcgactcgg gagaagtcgc cgtcgtgctc aagccgcacg cccccgatcc
     3481 gtaccgcccg caggtccgcg tgctgatcga tcgcgatggc cagtccatgg cgacgcctcg
     3541 cgatctgaac ctctgggagc ttcacgaggg cgagtccaga tcgattctga agccgctcga
     3601 ccccgagcag tacggcatcg atccgctggt tcatctgtaa aacggggtcg ggagtcattt
     3661 ctcgaggaca cttcccgagt ccaggagtcc tgtcggatca gtcagatcag agtgtcctca
     3721 agaaatgact cccgacccca ttacactact ggtacatgcg tcggaccgct tgcctgttcg
     3781 gccttcttct cgctctcgtc atcgtgatca gcgggctctc cgcacagagc tcgccgcgac
     3841 tcgtcgtaat cctcgccgtc gatcagcttc gcgcggacta tctgcagacc ttcaatcggc
     3901 actggcaggg gggattccgg ctcttgctcg acgaaggcct gaatttcgac aacgcccgat
     3961 acccgtacct cggaacggtg acgtgcgccg ggcattcgac gatcggcacc ggcacgcagc
     4021 cgcgcacaca cgggatggtc agcaacacct ggtgggatcg cgagtcacgc cgcatgatcc
     4081 tgtgcacggg cgaccccgac acgccggaca tcacctacgg ccgcccggtt cgatcgggca
     4141 acagcgcgaa gtggatgctc gcgcctacgc tggccgacga actgcgcgcc cagaggccgg
     4201 gggcgcgtgt cgtggcactg tcgctcaagt cgtacagcgc gatcggtctg gcgggacacg
     4261 cgggggatgc cgtcgtgtgg ttcgacgatc aggccggatc cttcgcgacg tctcgcgcgt
     4321 actcccccgg gccggtgcca gaggtgaagg cctttatcga cgggcatcct ttcgagggcg
     4381 accacggcaa ggtctggacg ctgtctgcgc cggcgagtac ctacgtgatg cgcgacgccg
     4441 gcgtcggtga acggcctctg cccggatggt ccggactgtt cccgcatccg ctcgtcggcc
     4501 gcagcgggcc cgactcacag ttcttcgacc tgtggcagtc cactccgttt gccgacgcgt
     4561 acctcgagcg catggccgaa tcgctgatcg acagctttgc cctcggccag cgcgacacga
     4621 ccgacttcct cggcatcagc ttctcggtgc tcgacgacgt cggacacagg ttcggacccg
     4681 agagccgcga gatcgaagag gtcctgcggc agctcgatgc gacgctgaaa cggctgatcg
     4741 ctcacctcga cgcgaaggtt ggacggctca actacatgct ggcactgacg gccgaccacg
     4801 gcgtcgcgcc catcgccatg gccccgcggg gcggccgcat cgccggcgag gacgttcgcg
     4861 aacggatcga cgagacgctc gccgcgcact tcggtccgct ggcccagggc tcgtacgtcg
     4921 aggccacgag cttcagcgac gtgttcctcg cgcctggcct tctcgatcgg ctcaggcagg
     4981 cgccttcgac gatgctggcg ctcgagcacg cgctgaaaga gattccgggc atcacgcacg
     5041 tgctgcgggc cgaccagctg tcggaccgga gcccggaccc ggttgtgaag acggccgcgc
     5101 tcagccacat cgaaaggcga agcggcgacc tcgtgctcgt gccaagggaa tactggtact
     5161 tcgccggccg ggccaatgcc accgcgacga cgcacgggac gctgcacgaa tacgaccagc
     5221 acgtgcctct gatcattttc ggcggcagcg cgcggcgggg gcacgatcga tcgacggcct
     5281 cgcctgcgga catcgcaccg acgctggccc ggttcgccgg aatccggatg tcgaaggccg
     5341 agggaaggaa cctcccggga acctggtgac gtagccgtcg atgtcgttca agcagcgagt
     5401 attggcagcc gttcggcgga ttccgcccgg gcgagtcgcc acctacggcg acatcgccgc
     5461 ccttgccggt tcgccacggg cgtgccgggc cgtcggaacg atcatgcggg actgccggga
     5521 cccggcaacg ccctgccatc gcgtcatcag cgccggtgga ctgctgggag gcttcggagg
     5581 cagcccgcac ctcaagcggg agctgctgcg cgccgaggcg ctcgacgttg gcgtccggcg
     5641 cgtccgcaat ttcgctgccg tccgctggca aggcgggaaa aaacaggccc gcgggggcag
     5701 atctggccac ttgggctgaa ccagccgggc cttcgacacg tcttcgacca tgtggcgttg
     5761 atgaaggacg tcgtcacgcg gcaacgcggc tcggtcagac ggccgtctaa cccatcagaa
     5821 gctgtgagag cagtcgacct ggaactggcg cagcgttgcc aacagggcga ctcccaggca
     5881 ttcgaggagc tgtatcgggc gcacgcgggg cggttgtaca acctggtgtt tcggatgacg
     5941 gcgtcggcaa ccgaggccga ggacgtcctg caggaggtat tcctccacgc ccaccggaag
     6001 ctggggagct tcaggggtga ctcgacgctg ggcacgtggc tgtaccggtt gacggtcaat
     6061 cactgcctcg acttcctgag gggtcggcag acgagaatga cgcgcgtcac cgaatcgctg
     6121 gacgacgagc gggcggcgga gccggcggcg gccgctccgg tggtcccgtc agccgtcagt
     6181 cgcatcgacc tcgagcgcgc tatcggcagg ctgcccgagg gatgccgcgc ggcgttcgtc
     6241 ctgcacgacg tccaggggtt cgagcatcac gaggtcgcaa ggcttctggg cgtgtcggag
     6301 gggacgtcga agtcgcaggt gcacaaagcg cgcatgaagc tgcgggggat gttgaacgag
     6361 acgaagtcat gacctgccag gactacgaaa tcgagctcgg cgactacgtg gacggcacgc
     6421 tcgaggggga cgcgcgctcg gctttcgagg cccatctctc gacgtgcggt cgctgcacgg
     6481 cgctcgtcgc cgacttcgga acgattctgg gggccgctcg ggtgctggag cccgaagttc
     6541 cgtcgccatt tctctggacg cggatctcgg ctgcgatcga gtccgagcgc gggcccgggc
     6601 gctgggggtt cacgtggcgg acgctcgcct cgtccgcagt ggccatgatg ctcgtcgcca
     6661 cgatgtcctg ggtgggcgct ggcctcgtgc ccgccaacga gcagggggtg aggctcgcgc
     6721 aggccgggcc gggcggggcc gagccgatct cggtcgcggc gcagttcgag ctggcggaag
     6781 cagattactc cagcgcgatc gcgcgcctgg agacgatcac gaaggccgag caagccgcgc
     6841 tcgacacgga cacggccggc gtattccgcg cccagctcac ggtcatcgac ggagccgtcg
     6901 gcgagagcaa ggcagcgctc gcgacggcgc ccggcgatca ggtcgcgcag cagagcctgt
     6961 tcgaggcgct gcgcagcaag ctcgagttgc tgcaggacat gctggcactc atcaacgaga
     7021 tgcggaaagg cgacccggaa ggcgccgcgc gcatcgtttc ggggttgaac taatgagact
     7081 gattcagtca agcgcgctcg tcgccctcgt catcgcggta ctgccgcctc cagcggccgc
     7141 ccaggcgccc tcgtgggtcg aggagctggc gaaagagatc gcggagaacg tcgaacggca
     7201 cgccgaacag atcgcccgct tcgtcgagca gcgtgtcgaa gcacagggcc gccgcggggg
     7261 acgcggagga actgagtaca ccgaatcgtt ctcgcggacc gttcggctcg gccgcaccgg
     7321 catgttcagc ctgcagaacg tcagcggcga catcgtgatc actggcggcg gcggcgacga
     7381 cgtgcgcgtg gatgccacca agcgcgtacg acaccccaac gaggccgagg caaaatcgat
     7441 tctgtcggcg gtgcagattc gcgtcgacga gggcagcggc cgcgtcgatg tccggaccga
     7501 ttaccccagc cgccgcaatt ggtccggcag cgtcgatttt caggtgaccg tcccgcggga
     7561 cgcgagcgtg aacgtccgca gcgtatccgg ggacgttcgc gtcacgaacg ccaacggcga
     7621 gcttcgcgca gaaaccgtga gcggcgacgt catcatctcg tcggcgcgcc gcgtcggcct
     7681 ggtcaagagc gtgtccggag atctccagat ctctgacgcc gaagccggag agctccgatt
     7741 cgaaagcgtc agcggcgacg tcgtggtcaa caagctgaag gcgaacgggc tcgacgtgaa
     7801 cgccgtgagc ggagacgtgc gtatcagcga cgtggaaacc gggcgcgcca cgttgcgggc
     7861 ggtcaccggg gacgtcgagt tcgccggccg cctcgcgcgc aacggccgat atcagctgca
     7921 gtcgcattca ggcgacgtgc ggatgactcc ggtcgacgtg cgcggcttcg cgctcgaagc
     7981 gacgacgttc agcggagaaa tccgctccga cttcgcggtg accgtgcagg gcacgcaggg
     8041 cgacccccag tcgaatcggc gcgtcgggcg ctcgattcgc gggtcgttcg gcgacggggg
     8101 cacgacgctc atgttgcagt ccttcagcgg cgacatcatt ctcattcgcc ggtagccggg
     8161 gaattcgtcg tcgtttttcc ggtcgcgcca tcggtgccgc ccggcgcacg atcggtgcat
     8221 gatcacgagc atgccaacgg cgtcccgcgt ctctcctcca ccggttccgc ttccgcgccg
     8281 ccaggaaatg gagcgtgcct accgggggtc tgacgcctcc tacgacggcg tcttctacct
     8341 tggcgtgcgc acgaccggca tcttctgccg gccgtcctgc cccgcgcgca agccaaagcc
     8401 ggagaacgtc gagttcttcg cgctccccaa ggacgccctg ttcgccgggt accggccctg
     8461 cctgcgctgc cggccgctcg acggcgacca ggccccgccc tgggtgtctc gcctgctgga
     8521 gcgcgtcgac aaacgcccga ccgcgcggat tcgggagcgc gacctgcgcg tcatgggcgt
     8581 cgagcccgcg cgcgtccgcc gctacttctc gacacgttac ggtctgacct ttcaggcgta
     8641 ttgccgggcg cggcggctcg cgcttgcctt cgagcgcatc cgcaacggag gctcgctcga
     8701 cgatgcggtg ttcgacacgg ggtacgagtc gcacagcggc tttcgcgacg cgtttcgaaa
     8761 ggcgttcggg catcccccgg gtcgatccgg cgacgccggc tgcatccgtc ttacgtggct
     8821 ggatacgccg ctcggtccga tgatcggcgg cgcgaccgac gccggcgtgt gcctgctcga
     8881 gttcgccgat cgccgaatga tcgagaacca gctcgcgacg ctgcggcggc gtttccgcgc
     8941 cggcctcgcg ccggcgagac acccgcacct ggccgccctc gagcgcgagc tcggcgagta
     9001 tttcgccggc acgcgccgcg ccttcagcgt cccgaccgac acgcgaggca cgccgtttca
     9061 aacgcgcgtg tggcgagcgc tcaacggcat tccgtacggc gagacccgat cctatcgcga
     9121 catcgcgcgg cagatcggcc gcccggccgc ggtccgcgcc gtcggccacg ccaacggcat
     9181 gaatccggtc gccatcgtcg tcccgtgcca ccgcgtcgtg aacaccagcg gcgcgcttgg
     9241 cggctacggc ggtggactct ggcgcaagcg gcggctgctg cacctcgaag cagcgccgc
//
LOCUS       DBNGSH010000323         6527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3761830, whole genome shotgun sequence.
ACCESSION   DBNGSH010000323 DBNGSH010000000
VERSION     DBNGSH010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3761830"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="AC16CY_07600"
     CDS             complement(<1..958)
                     /locus_tag="AC16CY_07600"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3353687.1"
                     /translation="MFRSVAQIVSWSALAVGVVLFLVTLARIDLDETLNSVRGLGLAF
                     PVLLLPSACWQLLRTWGWSVAFPDHARPTFSRLFRVRMASDAIGFFTVRGITGEPLKV
                     VLLYDRVSPSITTAAITLERMAFGVISLVIAGIVSWFAVRRLTMPGAWDAVFTVVSIA
                     TVVAVFILAQLARRRRGHYLGRGVQRIGRLTGRPLESSRIVRFILDVEDVLLELLRGD
                     RRRLIILTVLPIVCYLISAFEVWLVLWAIGEPIGITAALAIDTFVRLASIAYAPIPGG
                     LGALEASNAAPVAMLGLGGGGVLAFVRRVKGLVWAAIGLACYP"
     gene            complement(969..1640)
                     /locus_tag="AC16CY_07605"
     CDS             complement(969..1640)
                     /locus_tag="AC16CY_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3353688.1"
                     /translation="MAVRLFIGNLPYSASEADLRAHFAAVAEPSHVAMPVDRDTGRPR
                     GFAFVEFADRAVAEEVIRKFDAQPFQGRPLAVSEARAREDRPPRPPGGGFAPRPGGGG
                     FGGPRPGGFGGPRPGGGGFGARPGPGGMPAGPGGRDRSANFGPPAPPKRLRGKKTTQQ
                     EHKPRGPIKERTGGRIYAVDDLDDDSTVEIDDVATRAKDADDSTVEIDDVTTPAEDAH
                     EPDEE"
     gene            complement(1691..2608)
                     /locus_tag="AC16CY_07610"
     CDS             complement(1691..2608)
                     /locus_tag="AC16CY_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR3353689.1"
                     /translation="MRPDLSSLASMIEGRVVTRFAPSPTGYLHLGHVVHALYVWGVAR
                     ALGGHVVMRIEDHDRLRSRPEYESALVEDLGWLGFEPDSGASPRLRQSDTPAVYEEAL
                     ARLRRTHHVYACECSRKEIGSERYGGRCRDRGLREAPGRGLRVEMGREVESFEDGLLG
                     LQEQTPADQCGDLLVRDRDGQWTYQFAVTVDDLRQGISLVIRGADLTSSTGRQIALAR
                     MLGRARAPVFLHHPLIRDDSGNKLSKSASDTGVRELRAAGVDAAGVIGRAAFLVGLVD
                     RPRPLPAHEVPLHFLRSDPSAGPGLQVPN"
     gene            2634..3341
                     /gene="aat"
                     /locus_tag="AC16CY_07615"
     CDS             2634..3341
                     /gene="aat"
                     /locus_tag="AC16CY_07615"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR3353690.1"
                     /translation="MIPFLEIDDPFPPVERALREPNGLLAAGADLSPERLIDAYSRGI
                     FPWFGDDDPVLWWSPDPRMVLSTADLHVSHSLRKTIRSGRWRVTADTSFAQVMEGCAG
                     TRPGQDGTWITAEMMSAYDRLFSLGYAHSVETWMGERLVGGAYGVAIGRMFFGESMFS
                     AVTDSSKVALVALVRQLERWGFPCIDCQMSTSHLSSLGAREVPRASFLQQVDRLVRAA
                     PVPSPWRFDADLLVQLS"
     gene            3366..3686
                     /locus_tag="AC16CY_07620"
     CDS             3366..3686
                     /locus_tag="AC16CY_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR3353691.1"
                     /translation="MPGAHESTLTESFADQKVEPPRMWRVLLHNDDYTTQEFVVWVLE
                     TVFHKPRMEAVVIMMSVHRSGIGVAGVYTHDVAETKMKKTMQMAEQQEFPLLVTMEPE
                     LEHE"
     gene            3679..5922
                     /gene="clpA"
                     /locus_tag="AC16CY_07625"
     CDS             3679..5922
                     /gene="clpA"
                     /locus_tag="AC16CY_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HIR3353692.1"
                     /translation="MSKTPVPFAPETLETMKRAFRLAADRRHEEVGLEHLLLSITDEP
                     QGKQVLASCGANLDLLRRQLTDVLTKAYTPVLSATSVEPEPTLGFDRVVQQAMLHAVS
                     SSAKHVDSGSLLVFLLQEEDSHAAFFLRSQGVERLLLLRVLSHGAKSAPEAVPAGGDR
                     TAPPTDPLRAYATDLIARAAAGQIDPLIGRALEIERMIQVLCRRRKNNPLLVGEPGVG
                     KTALAEGLALRIHEGRVPEVLKNARVFALDLGALVAGTRYRGDFEERVKLVLDRLSKE
                     ENAILFIDEIHTLVGAGAASGGAMDAGNLLKPALAGGTLRCIGSTTFSDVKQSFDRDR
                     ALSRRFQKIDILEPSEDETLEILKGLRLHYESHHGVQYDDDALAAAVTLSARHLQDLH
                     LPDKAIDVLDEAGAAQKMLPPAERVARITASEIERIVARMARVPVQTVSSDDRLALER
                     LDEELRQVIFGQDGPIDEVASAIKLSRSGLRAPEKPIGNFLFAGPTGVGKTELARQLA
                     RIMGVEFIRFDMSEYMEKHAVSRLIGAPPGYVGYEEGGLLIDAIRKTPHAVLLLDEIE
                     KAHPDMFSILLQVMDHATLTDSHGRKADFRHIILIMTTNAGARDLSDRRLGFADAGQG
                     GSSRGAVERTFTPEFRNRLDAVVTFGALGTREIERVVDKQIEDVRGMVGARKVTIVLE
                     PAARTWLAEKGFDRAFGARPMARLIERVVKKPLSEMLLFGSLAQAGGEVRITVENDAI
                     RLVVSGQ"
     gene            5912..>6527
                     /gene="pdxH"
                     /locus_tag="AC16CY_07630"
     CDS             5912..>6527
                     /gene="pdxH"
                     /locus_tag="AC16CY_07630"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR3353693.1"
                     /translation="MASEGSGREYSGHPLTEGQSDPDPLEQFSRWYDQARTLEIDATM
                     MALATATRAGRPSVRNVLLKSVDARGFVFFTNYQSRKADELAETGRASLLFTWRTLER
                     QVRIDGAVERVSAAESDAYWDSRHIESRWAVYASRQSDVVDSREALEAAFDTARERYG
                     QSPPRPDWWGGYRVIPDEIEFWQGRSHRLHDRLRYRRQPDGSWYR"
BASE COUNT         1114 a         2152 c         2255 g         1006 t
ORIGIN      
        1 tcgggtagca cgccaggccg atcgccgccc acacgagacc tttgacgcgg cggacgaacg
       61 ccagcacgcc gccgcctccc agccccagca tggccacggg cgcggcattg gacgcttcca
      121 gcgcgcccag cccgcccggg atcggagcgt aggcgatgct ggccagccga acgaacgtat
      181 cgatggccag cgccgccgtg atgccgatcg gctcgccgat cgcccagagc acgagccata
      241 cctcgaacgc agagatcagg tagcagacga tgggcaggac ggtgaggatg atcagtcgcc
      301 gtcgatcgcc gcgcagcagt tcgagcagga cgtcctccac gtcgaggatg aagcggacga
      361 tgcgggacga ttcgaggggc cgcccggtca gacgcccgat gcgctggacg ccgcgcccga
      421 gatagtggcc cctgcggcgc cgcgctagct gcgccaggat gaacaccgcg acgacggtgg
      481 cgatcgagac gaccgtgaag acggcgtccc acgcgccggg catcgtcagg cgccggacgg
      541 cgaaccacga gacgatgccc gcaatcacca gcgagatcac accgaaggcc atccgttcca
      601 gcgtaatcgc cgcggtcgta atcgatggcg agacgcggtc gtagagcagc acgactttca
      661 gcggttcacc cgtgatcccg cggacagtga agaagccgat ggcgtcactg gccatccgca
      721 cgcggaacag gcgactgaac gtgggccgcg cgtggtcggg aaaggcgacg gaccatcccc
      781 acgtgcgcag cagctgccag catgcgctgg gaaggagcag gaccggaaag gcgagcccga
      841 ggccgcgtac gctgttcagc gtctcgtcga gatcgattcg ggcgagggtg acgagaaaga
      901 gaacgacgcc gaccgccagg gccgaccacg agacgatctg cgcaacggac cggaacacct
      961 acgagcgatt attcctcatc cggttcgtgg gcgtcctcgg ccggcgtcgt gacgtcatcg
     1021 atctcgaccg ttgagtcgtc ggcgtccttg gcccgcgtcg cgacgtcatc gatctcgacc
     1081 gttgaatcgt cgtcgagatc gtcgacggcg tagatccgtc cgccggttcg ttccttgatc
     1141 ggcccgcgcg gcttgtgctc ctgctgagtc gtcttctttc cgcgcagtcg tttcggcggc
     1201 gcgggcggtc cgaaattggc gctcctgtcg cggccgccgg gtccggcagg cattcctccc
     1261 ggtcctggac gtgcaccgaa gccgccgccg cccggacgag ggccgccgaa cccgcctgga
     1321 cgaggaccac cgaacccgcc gccgccgggc cgcggcgcaa agccgccgcc gggcggacgc
     1381 gggggacgat cttcgcgcgc gcgcgcctcg ctcacggcaa gggggcggcc ttgaaaaggc
     1441 tgcgcgtcga acttgcggat cacttcttcg gcgaccgcgc ggtccgcgaa ctcgacgaag
     1501 gcgaatccgc gtggacgacc cgtgtcgcga tccaccggca tggcaacgtg ggagggttcg
     1561 gcgacggcag cgaaatgggc tctgagatcc gcttcgctcg cgctgtaggg aaggttcccg
     1621 atgaacaaac ggactgccaa ggtctcctca gtgtaagacg ccgcccgata aggcggcgct
     1681 cgattataac tcagttcggg acttgcaacc ccgggccggc gctcgggtcc gagcgaagga
     1741 agtgcaaggg gacttcgtgg gcgggaaggg gtcttggccg gtccaccaac cccacgagga
     1801 acgcggccct cccgatgacc ccggcggcgt caacgcccgc ggcccgcagc tcgcgcacgc
     1861 cggtgtcact ggcggattta ctcagtttgt tgccgctgtc gtcacggatg agcggatggt
     1921 gcagaaagac gggggcccgt gcgcgaccga gcatccgggc gagcgcgatc tggcgtcctg
     1981 tcgacgaggt gagatcggcg cctcgaatca cgagcgagat cccttgccgc aggtcgtcca
     2041 ccgtcacggc aaactggtag gtccactggc cgtcgcgatc ccgaacgagc aggtctccgc
     2101 attgatccgc gggtgtctgt tcctggaggc cgagcagtcc atcctcgaac gactccacct
     2161 ctcgacccat ttcgaccctg agcccacgtc caggtgcctc tcgaaggccc cggtcccggc
     2221 accggcctcc gtagcgttcg ctcccgatct ctttgcggga gcattcacag gcgtagacgt
     2281 gatgcgtccg ccgcagcctg gccagcgcct cttcgtagac ggcgggcgtg tcgctctggc
     2341 gcaggcgggg gctggcgccc gagtccggtt cgaatcccag ccaccccaga tcttcgacga
     2401 gcgccgattc gtactccggg cggctgcgaa gacggtcgtg gtcttcgatt cgcatgacca
     2461 cgtgcccgcc aagggctcgc gccacgcccc agacgtacag cgcatggacc acgtggccga
     2521 gatggagata acccgtggga gagggagcga accgcgtcac gacgcggccc tcgatcatcg
     2581 acgcgagcga ggaaagatct ggacgcaagt ggcagaatct acggttagat tacgtgattc
     2641 cgtttctcga gatcgacgat ccgttcccgc ccgtcgagcg tgcgctgcgc gagccgaacg
     2701 ggcttctggc cgcgggcgcg gacttgtctc cggagcggct gatcgacgcc tacagccggg
     2761 gaatcttccc gtggttcgga gacgacgatc ccgtgctctg gtggagcccg gatccacgca
     2821 tggtgctgtc aaccgccgat ctgcacgtct cgcactcgct caggaagacg atccgatccg
     2881 ggcgctggcg cgtgaccgca gacaccagtt tcgcgcaggt gatggaagga tgtgccggga
     2941 ccaggcccgg ccaggacggc acctggatca cggccgagat gatgagcgcg tatgatcggt
     3001 tgttctcgct tggctacgcg cactcggtgg aaacgtggat gggggagcgc ctggtcggcg
     3061 gggcctacgg cgtcgcgatc ggacggatgt tcttcggcga gtcgatgttc agcgccgtga
     3121 ccgactcatc caaggttgcc ctggtggcgc tcgttcgcca gctcgaacgg tggggctttc
     3181 cctgcatcga ctgccagatg tccacgagcc acctgtcgtc gctcggcgcg cgcgaagtcc
     3241 cgcgggcgtc gtttctgcag caggtcgatc ggctggtccg cgcggcgccc gtcccctcgc
     3301 catggcggtt cgacgcggac ctcttggtac aattgagcta gctggtagcc ggtacacagc
     3361 ccaacatgcc tggtgcacac gagtccacac tgacggagtc gttcgccgac cagaaggtcg
     3421 agccccctcg catgtggcgg gtgctgctgc acaacgacga ctacacgacg caggagttcg
     3481 tcgtctgggt gctggagacg gtgttccaca agccccggat ggaggctgtc gtgatcatga
     3541 tgagcgtgca tcgctccggc atcggcgtgg cgggcgtcta cacgcacgac gtcgccgaga
     3601 cgaaaatgaa gaagacgatg cagatggcgg agcagcagga gtttccgctg ctcgtgacga
     3661 tggagcccga gctcgagcat gagtaagaca cccgttccgt tcgcgccgga aacgctcgag
     3721 acgatgaagc gggcgtttcg gctcgcagcc gatcgacgcc acgaagaagt cgggctcgaa
     3781 cacctgctgt tgtcgatcac cgacgagccg cagggcaagc aggtgctcgc ctcgtgcggc
     3841 gccaatctcg acctgctgcg ccggcagctc accgacgtgc tgacgaaggc gtatacgccc
     3901 gtgctgtcgg cgacctccgt cgagcccgag ccgacgcttg gcttcgaccg ggtggttcag
     3961 caggcgatgc tgcacgccgt ctcctcgagc gcgaagcacg tcgacagcgg aagcctgctg
     4021 gtctttctcc tgcaggagga agacagccac gcggcgttct ttctccgctc gcagggagtg
     4081 gagcggctgc tgctgctgcg cgtgctgtcg cacggggcga agagcgctcc cgaagccgta
     4141 cccgccggcg gcgatcgaac agcgccgccg accgatccgc tgcgcgccta cgcgacggat
     4201 ctgattgcgc gtgctgcggc gggccagatc gacccgctga tcgggcgggc gctcgagatc
     4261 gagcggatga ttcaggtgct gtgccgccgc cgcaagaaca atccgctgct cgtcggcgag
     4321 cccggggtcg gcaagacggc gctggccgag ggactcgcgc tgcgcattca cgaagggcgc
     4381 gtgccggagg tgctgaagaa cgcgcgggtc ttcgcgctcg atctcggggc gctggtggcc
     4441 ggcacccgct atcgcggcga cttcgaggag cgggtcaagc tggtgctcga ccggctgtcg
     4501 aaggaagaga acgcgattct cttcatcgac gagatccaca cgctggtcgg cgcgggcgcc
     4561 gcgtcgggcg gtgcgatgga tgccggcaac ctgctcaagc cggcgctcgc cggcggaacg
     4621 ctgcgctgca tcgggtcaac gaccttcagc gacgtcaagc agtcgttcga ccgcgaccgg
     4681 gcgctgtccc ggcgcttcca gaagatcgac atcctcgagc cctcggagga cgagacgctc
     4741 gagatcctca agggccttcg cctgcactac gaatcgcacc acggcgtgca gtacgacgac
     4801 gacgcgctgg cggccgccgt gacgttgtcg gcgcggcacc ttcaggatct gcacctgccg
     4861 gacaaggcga tcgacgtgct ggacgaagct ggcgcggcgc agaagatgct cccgcccgcg
     4921 gagcgcgtgg ccaggatcac ggcctcggag atcgagcgca tcgtcgccag gatggcgcgc
     4981 gtgccggtgc agaccgtgtc cagcgacgac aggctcgcgc tcgagcgcct cgacgaggag
     5041 ctccggcagg tgatcttcgg gcaggacggc ccgatcgacg aagtcgcctc ggccatcaag
     5101 ctgtcgcggt cgggcctgcg ggcgccggag aagccgattg gcaactttct cttcgccggg
     5161 ccgaccggcg tgggcaagac ggagctggcg cgccagctcg ccaggatcat gggagtcgag
     5221 ttcatccgct tcgacatgtc cgagtacatg gagaagcacg ccgtgtcgag gctcatcggg
     5281 gccccgcccg gttatgtcgg ctacgaagaa ggcgggctgc tcatcgacgc catccgcaag
     5341 acgccgcacg ccgtgctcct gctcgacgag atcgagaagg cgcacccgga catgttcagc
     5401 atcctgctgc aggtgatgga tcacgcgacg ctcaccgatt cgcacggccg caaggccgac
     5461 ttccgccaca tcatcctgat catgacgacc aacgccggcg cgcgcgatct gtcggaccgc
     5521 cgcctcgggt ttgccgacgc ggggcaaggc ggatcgtccc gcggcgcagt cgagcggacc
     5581 ttcacgcccg agttccgcaa ccgcctggac gccgtcgtga cgttcggcgc gctcggcacc
     5641 agggagatcg aacgcgtcgt ggacaaacag atcgaggatg tgcgcggcat ggtcggcgcc
     5701 cggaaggtca cgatcgtgct cgagcccgcg gcgcggacgt ggctggccga gaaaggcttc
     5761 gaccgtgcgt tcggcgcccg acccatggcg cgcctcatcg agcgggtcgt caagaagccg
     5821 ctgtcggaga tgctcctctt cgggtcactg gctcaggccg gcggcgaggt gcgaatcacc
     5881 gtggagaacg acgccatccg gctggtggtg agtggccagt gagggctccg gccgcgagta
     5941 ttccggccac ccgttgacgg aagggcagtc cgacccggat ccgctcgagc agttctctcg
     6001 ctggtacgac caggctcgta cgctcgagat cgacgcgacg atgatggcgc tggccacggc
     6061 gacgcgcgcg ggccgtccat cggtccgcaa cgtcctgctc aagagcgtgg acgcacgcgg
     6121 cttcgtgttc ttcaccaact accagagccg gaaggctgac gagctggccg agacgggccg
     6181 cgccagcctg ttgtttacgt ggcgcacgct ggagcgccag gtgcgcatcg atggcgccgt
     6241 ggaaagagtc agtgcggccg agtccgacgc ctactgggac tcacggcaca tcgagagccg
     6301 ctgggccgtc tacgcgtcga gacagagcga cgtcgtggac agccgcgaag ccctcgaggc
     6361 ggcgttcgac accgcacgcg aacgttacgg ccagagcccg ccgcggcccg actggtgggg
     6421 cgggtaccgg gtgatccccg acgagatcga attctggcag ggccgatcgc acaggctcca
     6481 cgatcgcttg cggtaccgaa gacagcctga cggctcgtgg taccggg
//
LOCUS       DBNGSH010000324         7445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1045317, whole genome shotgun sequence.
ACCESSION   DBNGSH010000324 DBNGSH010000000
VERSION     DBNGSH010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1045317"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2269
                     /locus_tag="AC16CY_07635"
     CDS             <1..2269
                     /locus_tag="AC16CY_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3353694.1"
                     /translation="RPGDNAEVWERVIGRLRAGSMPPPGRPRPDAATYRGVAALLERE
                     IDRAWTAYPKPGRIAAVHRLNRTEYTNVIRDLFALDVDVTSLLPGDETADGSFDNLAD
                     ALSISTAHLERYLSVARHVTRLATGLPPTSPRVETFEIPLHVIQDDRQSEDLPFGSRG
                     GIAIPYHFPVDGEYLVKIRLQRQYQDYLKGMGWPQQLDVRLDGRLVKRFTVGGDAKGR
                     PAALSYAGDGEPGFAGDPEWERYMQLTGDAGLEVRITAEAGPRTVGVSFVRELWEPEG
                     LPHPPQRGRTIANDNDYMGYANVGAIQIGGPYGAVGPTRQTPSRRAIFVCRPRSRTEE
                     HGCATRILSNLARRAYRRPVTKADLEGLLDFFDRGRAEGGSFDAGVQLALERVLVDPD
                     FLLRTYRDSPARDTRYRLSDLEVASRLSFFLWSSIPDEHLLDLAERKRLTRQPVLEQE
                     VRRMLADPRAAAALVDGFVAQWLNLRRVGEVVVDPDRYPTYDESLLQAFERETKLFVG
                     STLREDRSVSQLLDAKYTFVNERLARHYGIPGIYGSRFRRVSLPNADQRGGLLAHGSV
                     LATTSYPDRTSPVLRGKFLLNNIFGMPAPPPPAGVDTNLAETKPNVQPPTMRERLAQH
                     RTNPSCAGCHSVIDPLGFALEQFDAIGAWRRVDESGRAVDASASLLDGTRIEGLAGLR
                     AVLLEEPEQFPRTVTEKLLTYALGRRLEHYDRPAVRTIVRDAAANDYRWSALITNIVT
                     SPPFLMREAAGGDSK"
     gene            2266..3606
                     /locus_tag="AC16CY_07640"
     CDS             2266..3606
                     /locus_tag="AC16CY_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3353695.1"
                     /translation="MTFLTGKALPRRTVLRGLGAAVSLPLLDAMLPGSSRRVHAAATR
                     PHRFLAFYVPNGMAMEFWTPKGEGRAFELSPILEPLAPFRDQMLVLSGLNASWNYIHA
                     GASGSFLTGTTRGGRNEVEILADVSVDQLLARQFAKETQVASLELAMDAPANAGACTG
                     ILSCAYTHTISWRSATQPLPMEHNPRAVFERLFGDSGSTGRASREARQRQQKSLLDSV
                     MEKLSHLRRTLGVEDQVKVEQYTEAVRDVERRIHKAEEQRDVELPTLDQPQGVPPVFE
                     DHLALMLDLQLLAFQSDLTRVMTFMIGKEQSARPYPQIGVPEAHHPLSHHNDVPELIA
                     HMSKINRYHTELFSKYLARLRATPDGDGSLLDHMTILYGSGISNSTRHSGQNLPLLLV
                     GGGSGRLEGGRHLKYSDKPTIANLLVTLMDKLDVPVEKIGGSTGKLPLETLSGV"
     gene            3749..5107
                     /locus_tag="AC16CY_07645"
     CDS             3749..5107
                     /locus_tag="AC16CY_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3353696.1"
                     /translation="MSLLFAGAVHAAHPLVDAARNADHDSIRRLLESKIDVNAAEADG
                     TTALHWASYRDDVESAELLIRAGANVNAANDLGATPVWTASLNGSVAMVRRLLEAGAN
                     PDAALLLGETAVMVAARSGHAGVVQQLLARGAHVNARAARGQTALMWAVAQKHPDVVR
                     VLLAHGADVRARSDSWSQVMAVSPHGHPEYNRAIPFGGNTPLMFAARVGDLESAKLLV
                     ASGADVNDADAWGISVVVLAAHSGFRDLVEFLLESGADANSSAAGFTALHEAVMRRDE
                     GLVRTLLAHGADPHARLQAWTPTRRQSTDLHFEPELVGATPFWLAARFVQPRVMRLLV
                     EFGADPLFVHRSDRVVDGREGRAYDHRYDATTALMAALGMGGGGAAWVPPDRSQREVL
                     TLETVRLAMEMGIDVNAVNTDGRTALDAAKALKYETVVAYLVEKGAKPGVKDDQDEQA
                     PK"
     gene            5137..5358
                     /locus_tag="AC16CY_07650"
     CDS             5137..5358
                     /locus_tag="AC16CY_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353697.1"
                     /translation="MRSVADGLRRDTARTVAEPPAMDRIALALRLGDEDVARYREAHG
                     VSDAEASRALARSRAVGRLPSRSNDPPGQ"
     gene            5950..6633
                     /locus_tag="AC16CY_07655"
     CDS             5950..6633
                     /locus_tag="AC16CY_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3353698.1"
                     /translation="MIWKRALVLGLLGVFLSVAPAAAQRVEVSGIFGWSLSDGVTFDE
                     VLAQDGNLYNEVDLKDSFSWGFSVGVLTSEQVEVGFLYNQQMSELQVKGAGLGGAAEV
                     VVGDMNVSNYHGYVAFNFGDTDATVRPYIMIGFGATNYPSVDFTTSTGLQLSTLGVTQ
                     FSTTWGAGIKAFPSPNVGVRAGIQWTPTYIKSDAEGWWCDPWWGCYVVGDAQYSNQLQ
                     FNGGVVVRF"
     gene            6658..7212
                     /locus_tag="AC16CY_07660"
     CDS             6658..7212
                     /locus_tag="AC16CY_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353699.1"
                     /translation="MIGRTIGVAAVVALLFPGVAPAAQKPVTLGEAISETFTIEAINY
                     ADRIVTLKDKDGFSEDLVAGPEVKRFDALKVGDRVTFRYHESLVTAIRRADAAKKAPE
                     AVSVNRTPGTKPGGTVSQQMTTVVTVEAIDAKVPSVTIKTADGRRMSFKVENPKNLAG
                     YKAGDNVEITYTRALAVSVEPAKK"
BASE COUNT         1319 a         2361 c         2506 g         1259 t
ORIGIN      
        1 acggcctggc gacaacgccg aggtgtggga gcgtgtgatc ggcagactcc gagcaggctc
       61 gatgccacct cccgggaggc cgcggcccga cgcagccacg tatcgaggcg tggccgcgct
      121 gctcgaacgc gagatcgatc gggcgtggac ggcatacccg aagccgggca gaatcgcggc
      181 ggtgcaccgg ctgaatcgca ctgagtacac caacgtcatt cgagatttgt tcgcgttgga
      241 cgtcgacgtg acgtcgctct tgccgggcga cgagacggcc gacggcagct tcgacaacct
      301 ggccgacgcc ctttcgattt caaccgctca cctcgagcgg tatctctcgg ttgcccgtca
      361 cgtcactcgt ctggcgacgg gcctgccgcc aaccagtccc agggtcgaga cgttcgagat
      421 cccgctgcac gtgatccagg acgatcgaca aagcgaggat ctgccgttcg gatcgagagg
      481 cggcattgcc attccctacc actttccagt cgatggcgag tacctcgtca agatccgtct
      541 gcagcgccag tatcaggact atctcaaggg aatggggtgg ccgcagcagc tcgacgtccg
      601 tctcgacggc aggcttgtca agcgattcac cgttggaggt gacgccaagg gtcggccggc
      661 tgcgctcagc tatgccggcg acggggagcc gggctttgcc ggtgatcccg aatgggaaag
      721 gtacatgcag ctcaccggcg acgccgggct ggaggtccgc attaccgctg aagccggacc
      781 tcgtacagtc ggcgtctcgt tcgtcaggga gctgtgggag cccgaaggcc ttccacaccc
      841 tccgcagcgg ggcaggacga tcgcgaacga caacgactac atgggctacg ccaacgtggg
      901 cgccatccaa atcggaggcc cgtacggtgc cgtgggtccg acgcggcaga cgccgagccg
      961 acgcgcgatc ttcgtctgcc gtccgcggtc caggaccgag gagcacggct gcgcgacgcg
     1021 catcctgtcg aatctcgcgc gccgcgccta tcgccggccg gtgacgaagg cggacctcga
     1081 ggggctgctc gacttcttcg accgtggacg cgccgagggt ggaagtttcg atgccggagt
     1141 gcaactggcg ctcgaacggg tgctcgtgga cccggatttc ctgctgcgta cgtatcgcga
     1201 ctccccggcc agggacactc gctatcgcct gagcgatctc gaggtcgcat cccggctgtc
     1261 gttcttcctc tggagcagca tcccagacga gcacttgctc gacctggccg agaggaaacg
     1321 tctcacccgc cagccggtcc tcgaacagga agtgcggagg atgctggccg atccgcgcgc
     1381 ggcagcggcc ctggtggacg gcttcgtcgc ccagtggttg aacctgcggc gcgttggcga
     1441 ggtcgtggtg gacccggacc gatatcccac ctacgacgag agcctgctgc aggccttcga
     1501 gcgcgagacg aaactgttcg tcggcagcac gctgcgcgag gaccgaagcg tttcgcagct
     1561 gctggacgcc aagtacacgt tcgtcaacga gcggctggcc aggcactacg ggatcccggg
     1621 gatctatggc agccgttttc gacgagtgag cctcccgaac gccgatcaac gtggcgggct
     1681 cctcgcgcac ggatcggtgt tggccacgac gtcctatccc gatcgcacgt cgccggttct
     1741 gcggggcaag tttctgttga acaacatttt cggcatgccg gcgcctccac cgccggcggg
     1801 cgtcgatacc aacctggctg aaacgaagcc caacgtccag ccgccgacca tgcgcgagcg
     1861 gctggcgcag caccgcacga acccgagttg tgccggctgc cactcggtga tcgatccact
     1921 cgggttcgct ctcgaacagt tcgatgcgat tggtgcgtgg cggagggtcg acgaatcggg
     1981 cagggccgtc gacgccagcg cgtccctgct cgacggcacg aggatcgaag ggcttgccgg
     2041 gctgcgtgcc gtgctgctcg aggagcccga gcaattccct cgcacggtga ccgagaagct
     2101 gctcacctac gcgctcggcc gtcgattgga gcactacgat cgcccggccg tgcggacgat
     2161 cgttcgtgat gccgcggcga acgactaccg ctggtcggcg ttgattacga acatcgtgac
     2221 gagccccccg ttcctgatgc gggaggcggc cggaggagac agcaaatgac gttcctcacc
     2281 ggaaaggcgc tgccgcgccg cacggtcctt cgagggttgg gggcggcggt gtcactgccg
     2341 ttgctggacg cgatgctccc cggctcttca cggcgtgtgc acgcggccgc cacgcggccc
     2401 catcggtttc tcgccttcta cgtgccgaat ggaatggcca tggagttctg gacgccgaag
     2461 ggtgagggac gcgccttcga gctctcgccg atcctggagc cactggcgcc gttcagagac
     2521 cagatgctgg tgttgtcagg gttgaacgcg agctggaact acattcacgc cggcgcctcg
     2581 ggatccttcc tcacgggcac gacgcggggg ggtcgaaacg aagtcgagat cctggcggat
     2641 gtctctgtgg accagttgct cgccaggcag ttcgcgaagg agacgcaggt cgcctccctc
     2701 gagctcgcga tggacgcgcc ggccaacgcg ggcgcgtgca ccgggattct gagctgtgcc
     2761 tacacccaca cgatctcgtg gcgcagcgcg acccagcccc tcccgatgga gcacaacccg
     2821 cgtgccgtat tcgagcgtct gttcggcgac agcggcagca cgggacgagc gtcgcgcgag
     2881 gcgcggcagc gtcagcagaa gagcctgttg gactcggtga tggagaaact gtcccacctt
     2941 cgcagaacgt tgggcgttga ggaccaggtc aaggtcgaac agtacaccga ggcggttcgt
     3001 gacgtcgaac gaaggatcca caaggccgag gagcaacgcg atgtcgagct gccgaccctc
     3061 gaccagcctc agggcgtgcc gccggtgttc gaggatcacc tcgccctgat gctcgacctg
     3121 cagctcctgg cgtttcaatc cgacctgact cgtgtcatga cgttcatgat cggcaaagag
     3181 cagagcgcgc gcccgtaccc gcaaatcggt gtgcccgagg ctcaccaccc gttgtcccat
     3241 cacaacgacg taccggagct catcgcgcac atgtcgaaga tcaaccggta tcacaccgag
     3301 ctcttttcga agtacctggc cagactccgc gccacgcccg atggcgacgg ctcgctgctc
     3361 gaccacatga cgatcctgta cggctcgggc atctccaaca gcacacgcca ctcgggtcag
     3421 aacctgccgt tgctcctggt cggcggcggc tcggggcgcc tcgagggcgg ccgacatctg
     3481 aagtactcgg acaagcccac gatcgcgaac ctcctggtga cgttgatgga caagctcgac
     3541 gtaccggtgg agaagatcgg cgggagcacc ggcaagctgc cgctcgagac actgtcgggg
     3601 gtgtgagggc aagggggcga gggggcaggg ggcagggggc agggggctag ggggcagggt
     3661 ggaaacagcg ctcggtcgca gccgtagagc gggggcttca gcccccgcgt tgacgcgcag
     3721 gctgaagcct gcgctctacg ctgtctgctt gtcgctgctc ttcgccggtg ccgtgcatgc
     3781 cgcacacccg ctcgtcgacg cagccaggaa cgccgaccac gacagcatcc gtcgtctgct
     3841 ggaatcgaag atcgatgtca acgcagccga ggcggacggc acgacggcgc tgcactgggc
     3901 gagctaccgg gacgatgtgg agagcgccga gctcctgata cgagcgggcg ccaacgtgaa
     3961 cgctgcaaac gaccttggcg caacgccggt ctggacggcc agcctcaatg gcagtgtcgc
     4021 aatggtgcga cgcctgctcg aagcaggtgc caacccggat gccgcgctgc tgctcgggga
     4081 aacagcggtg atggtggccg cccggtccgg ccacgccggc gtcgtccagc aactgctggc
     4141 gcggggcgcg cacgtgaatg cacgcgccgc aaggggccag acagcgctca tgtgggcggt
     4201 ggcccagaag cacccggacg tcgtccgcgt gttgctcgcg catggcgcag atgtgcgcgc
     4261 acgctcggac agttggagcc aggtgatggc cgtctctccg cacggccatc ccgagtacaa
     4321 ccgagcgatc ccgttcggcg gcaacacgcc gttgatgttc gcggcccgcg tgggcgatct
     4381 cgagtcggcg aagctcctcg ttgcctccgg cgccgacgtg aacgatgcgg acgcgtgggg
     4441 catcagcgtc gtagtcctgg ccgcgcattc gggctttcga gatctcgtcg agttcctgct
     4501 cgagagcggt gccgacgcga acagctccgc ggcgggcttt accgcgctcc atgaggccgt
     4561 catgcgccgg gacgaagggc tggttcgcac gctcctggcc cacggcgccg atccgcacgc
     4621 acgcctgcag gcctggaccc cgacgcgacg ccaatcgacg gatcttcatt tcgagcccga
     4681 gctggtggga gcgacaccgt tctggctggc ggcccggttc gttcaaccgc gcgtgatgcg
     4741 cctgctcgtg gagttcggtg cggatccgct gttcgtccac cggagcgaca gagtcgtgga
     4801 cgggcgtgag gggagggcct acgaccatcg gtacgacgcc acgacggcgc tgatggcggc
     4861 gcttggcatg ggcggcggtg gggccgcgtg ggttccaccc gacaggagcc agcgcgaggt
     4921 actcacgctc gagacggtca ggctcgcgat ggagatggga atcgacgtca acgccgtcaa
     4981 taccgacggc cgcacggcgc tcgatgcggc gaaagccctc aagtacgaaa ccgtggtcgc
     5041 ctatctcgtc gagaagggcg cgaagcctgg tgtgaaggac gaccaggacg aacaagcacc
     5101 gaagtaacga ttcgtctccg ccgttatact ggatccatgc gatcggtggc cgacggtctt
     5161 cggcgcgaca cggcgcgcac ggtggcggaa cctccagcca tggatcgcat cgcgctcgcg
     5221 ttgcgcctgg gagacgagga tgtcgcacgc taccgtgagg ctcacggtgt gagcgacgcc
     5281 gaagccagcc gggcgctcgc gcgctcccgc gcagtcggac gtctgccatc gcgctcgaac
     5341 gatccccctg gccagtgacg ctcctcgtgc ctcgcgcgcg acgttgtgat ccaagggctg
     5401 aatgtctccc tggtcgcggt gcggggctgt aggtcgctcc gcaaaaccga atctagttca
     5461 ggcacgtcag cttcgctgcc gaccaacgag ctgtgccaat acgctcacta cggttttgct
     5521 ccggacctac agccccgcac cgctgcctga ggtcgggcaa aacagagccc gacaccgtgg
     5581 ctgacgcacc agaacgtgtt gtggactaga ccgccgagct gaaacctccg gtcgacgaac
     5641 gcatcggact gctcccagtt cgcagccgca aaatctgggc atcgcttcgc ccccgcgact
     5701 gaccgaagtg cgcccatcag ctgagctgga gccgcatttg cgtgttcaat cgggcggcaa
     5761 aggcggaatt tcggtacacg cgttgcctat ccgaaggata attttcaccg cgtcgcctgt
     5821 ttcgggccgg cattgcccat gtcggtggcg gcttcgcgct gaaattttcg gaggcgcctc
     5881 cgaaaattca gcggtgtacg actggtgaag gctgcgccgg gatgggcgtt cggcggatgg
     5941 aggaaagaga tgatttggaa acgcgcgtta gtgttgggcc tgctcggagt gttcctgtca
     6001 gtggcgccgg ccgcagccca gcgagtcgag gtgagtggca tcttcggctg gtcgttgtcg
     6061 gacggtgtga ccttcgacga ggtactggct caggacggca acctgtataa cgaggtggac
     6121 ctcaaagact ccttctcctg gggcttcagc gtcggcgtgc tgacgagcga gcaggtcgaa
     6181 gtgggcttcc tgtacaacca gcaaatgagc gagttgcagg tcaagggggc tggtcttggt
     6241 ggtgcggccg aagtcgtcgt gggcgacatg aacgtgagca actatcacgg atacgttgcg
     6301 ttcaacttcg gcgatacgga tgcgacggtg cgcccctaca tcatgatcgg cttcggcgcg
     6361 acgaactacc cgagcgtcga cttcacgacc agcacgggtc tgcagctttc gacgttgggc
     6421 gtgacacagt tctcaacgac gtggggtgcc ggcatcaagg ccttcccgtc tccgaacgtg
     6481 ggcgtgcgtg cgggaatcca gtggacgccg acctacatca agtcggacgc cgagggctgg
     6541 tggtgcgacc cgtggtgggg atgctacgta gtaggtgacg ctcagtattc gaatcagctg
     6601 cagttcaacg gcggcgtggt tgttcgattc taggatcggg ttccaggaga tcgaaagatg
     6661 atcggaagaa ctatcggcgt cgcggccgtg gtggcgcttc tctttccggg tgtcgctccg
     6721 gctgcccaga agccggtgac cctcggcgag gccatcagcg agacgttcac catcgaggcc
     6781 atcaactacg ctgatcgcat cgtcacgctc aaggacaagg acggcttcag cgaagacctc
     6841 gtggcaggtc cggaggtgaa gcggttcgac gcgctcaagg tcggagatcg tgtcaccttc
     6901 cggtaccacg aatccctggt cacggcgatt cgacgggccg acgcggccaa gaaggcgccg
     6961 gaagccgtct cggtgaatcg cacaccgggg accaagcccg gaggtactgt ttcgcagcag
     7021 atgaccaccg tcgtcaccgt ggaggcgatc gacgccaagg tgccgtcggt gacaataaag
     7081 accgccgacg ggcgacggat gtccttcaag gtcgagaacc ccaagaacct cgccggctac
     7141 aaggccgggg acaacgtcga aatcacctat acgcgggccc ttgcggtcag cgtcgaaccc
     7201 gcgaaaaaat agccgtcgat aagatcggtt atgtcttgca gctcgggctg gcacggcccc
     7261 cggggccgtg ccagccgggt tgccggatgg gagccttttt caatgcagat caggtcacgt
     7321 cgttcccctg ctgtggccct cggtctcgtt gcgtcgttga cgatccctcc gggtgtggca
     7381 tccgggtggc agtcgaaaac cacacccgcc accgtcccgc cccccggcac aaatgccgac
     7441 accgg
//
LOCUS       DBNGSH010000325         1505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3252323, whole genome shotgun sequence.
ACCESSION   DBNGSH010000325 DBNGSH010000000
VERSION     DBNGSH010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3252323"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..980
                     /locus_tag="AC16CY_07665"
     CDS             162..980
                     /locus_tag="AC16CY_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353700.1"
                     /translation="MPFRPARVFAIVVMMSAPLAAQWPSYPKSGAPRTPDGKVRLDAP
                     APRTADGKPDLSGVWETVRTGPGQAITGPDVHPLKRPSQFWNIGSGLPGDLPFQPWAA
                     ALREKRVADNSRDNPDPHCLPIGLTQLHNHPQPRKIIQTPTLVLILYEANDGIRQIFL
                     DGRPLPTNDPQPWWYGYSVGRWDGDTLVVDTSGFRDGGWLDVNGAPLTDAARMTERFR
                     RVNYGTLEIDVTVDDPKAYTRPWTARTIRQQLLPDDELIEFVCKENEKSTQRFQ"
     gene            complement(1023..>1505)
                     /locus_tag="AC16CY_07670"
     CDS             complement(1023..>1505)
                     /locus_tag="AC16CY_07670"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3353701.1"
                     /translation="LVTRGVLLDIAALKRVPVLAETYEITPQDLQQALSAQKLTLQPG
                     DAVLIHTGWGTHWGTDNARYQRSSPGLGTAAAEWLAKQDPMLVGADNTAVEISPNPDK
                     QLAGPGHQIFLVVNGIHLLENLRLDELAARRVYEFALIVEPLKIQGGTGSTVAPIAIR
                     "
BASE COUNT          257 a          507 c          489 g          252 t
ORIGIN      
        1 gcgaggatct gcgccttgta gacgtacaag ctgtcaggcg ccgtcacgtg tccgtgcgag
       61 tcgatgatca ccgctgcctc cgcgtggtgt ggagccggat tgtatcagca ggtgtcctcg
      121 ccctgcccaa cccacgctga tcaggttaga cttcttcacg agtgccattc cgaccagccc
      181 gtgttttcgc catcgtcgtc atgatgtcgg cgccgctcgc ggcgcagtgg ccgtcgtatc
      241 cgaagtccgg cgcgcctcga acgccagacg gaaaagtgag gctcgatgcg ccggcgccgc
      301 ggaccgccga cggcaagccc gacctctccg gtgtctggga aacggttcgc acgggcccgg
      361 gtcaagccat caccggaccc gacgtccacc cgctcaagcg gccgagccag ttctggaaca
      421 tcggctccgg cctccccggc gacctcccgt ttcagccgtg ggccgcagcg ttgcgcgaga
      481 aacgcgttgc cgacaacagc agggacaatc ccgaccccca ctgcctgccg atcggtctca
      541 cgcagctcca caaccatcct cagccgagga agatcatcca gacgccgaca cttgtcctga
      601 tcctttacga agcgaacgat ggcatccggc agatcttcct ggacggccgg ccgctcccga
      661 ccaacgaccc tcaaccctgg tggtacgggt actccgtcgg ccggtgggat ggcgacacgc
      721 tcgtagttga cacgtccggc ttcagggacg ggggatggct cgatgtcaac ggcgcgccgc
      781 tcaccgacgc ggcaagaatg accgagcggt ttcggcgcgt gaactacgga acgctcgaga
      841 tcgacgtcac cgtcgacgat ccgaaagcgt acacgcgccc gtggaccgcg cgcacgatcc
      901 gtcagcagct cctgcccgac gacgagctga tcgagttcgt gtgcaaggag aacgagaagt
      961 cgacgcagcg ctttcagtag gtgctcggag gtacgtagtg tccggcttca gccgaccccc
     1021 gtttaccgga tggcgatcgg cgcgaccgtc gagccggtgc cgccctggat cttcagcggc
     1081 tcgacgatga gcgcgaactc gtagacgcga cgcgcggcca gttcgtccag ccggagattc
     1141 tcgagcaggt gaatgccgtt gacgacgagg aagatctggt ggccgggacc cgcgagctgc
     1201 ttgtccgggt tcggggagat ctcgacagcg gtgttgtcgg cgccgacgag catcggatcc
     1261 tgtttcgcca gccactcggc ggcggccgta ccgagaccgg ggctcgaccg ctgatagcgg
     1321 gcgttgtccg ttccccagtg cgtgccccag ccggtgtgga tgagcaccgc gtcgcccggc
     1381 tgcagcgtca gtttctgcgc cgacagcgcc tgctgaagat cctgcggcgt gatctcgtag
     1441 gtctccgcga gcacgggcac gcgcttgagc gcggcgatgt cgagcagcac gccgcgcgtc
     1501 acgag
//
LOCUS       DBNGSH010000326         3748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1894142, whole genome shotgun sequence.
ACCESSION   DBNGSH010000326 DBNGSH010000000
VERSION     DBNGSH010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1894142"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="AC16CY_07675"
     CDS             complement(<1..1365)
                     /locus_tag="AC16CY_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353702.1"
                     /translation="MNVRALVLVLMLVCFPSAAASNPQDPGTQGTTPDPPPDSTPSLT
                     WHGERIYFGLDLSLASVQDESLRESMGRERQVKLAFVIFSAYGDLGDHISYRIEANPA
                     DDAVVPRPYIPSLEDRRTYFFPNQPDFPGSRGVSSDPEGLYKVDDYQHTGLDPIIQQG
                     ALRIGWVDIHSASRRFGLRGGRFYVNQGMGVNDLVWFTAKDLTHIQRVNFQADNGLAL
                     YYNRGRFRAELAAITGNANPYHDYGYFDFTDPAEDKNSALGAVGTLRATFGRSFAGVS
                     YRKNYVNSKIEDTASIQLSKHTDNALVVFGRWEPKEFVRVFGEYAHYTWGVTPSSAEI
                     LPGPPIKTPVPKPGFYVGADLFAPKTGWARFGMTIMYEQISRDDALVAWAAANALYGV
                     TLGETQTSTIVKAHAKLLDRVTAFFFYNDLENPFPELSAIKAISGPGSERVVENYKYG
                     LGLRL"
     gene            1386..2996
                     /locus_tag="AC16CY_07680"
     CDS             1386..2996
                     /locus_tag="AC16CY_07680"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3353703.1"
                     /translation="MRDAPKPRTRARPHASARIGEVLRKILIANRGEIAVRVIRACRD
                     LGVPSVAVFSDCDRASRHVRMADEAVAIGADPPGESYLRIDRILDAARASGADAVHPG
                     YGFLSENEAFAAACRDAGLVFIGPTPEAIGRMGSKTTARKIASAAGVPVVPGTEEPYG
                     PEATDDEMARAATRIGYPVLVKAVAGGGGKGMRLVSRPEELRSAIRTARSEAATAFGD
                     SAVYIERRLTRPRHIEVQILADRHGAVQAFVERECSIQRRHQKVVEESPSPVVSRELR
                     DRLSEAAVAVAKAAGYTNAGTVEFLLDEDGRFYFLEMNARLQVEHPVTEMVTSVDLVS
                     WQVRLAQGEPLTLPSSHTLVPLGHAIECRVYAEDPDAGFMPSPGRITGLRVPDGPGIR
                     DDSGVEAGSDVPIFYDPLLSKLVSWGEDRPQATARMRRALDEYEVHGIRTTLPFFKWL
                     LARPEFVEARFHTGFLDELLQQRAGEPFGAADPSFEEVAAVAACLVEASHSPQAVAQA
                     TRSIQALQRMGPVVRGWKARARVEGLRE"
     gene            2993..3592
                     /locus_tag="AC16CY_07685"
     CDS             2993..3592
                     /locus_tag="AC16CY_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR3353704.1"
                     /translation="MRFEVETGGRRRTIDLSRSGADWIVRVDARQLSASLAQAGARWS
                     LLLRQLESEPLASRPVHGGLASSHEIAIEPRARGVRVVHVDGREIAVSILDPRSRAAR
                     DRGPADVAGPPGRRSAQGEGGSAPREGGSVVVSPMPGRVVKVLVAPGDVVAARQGLVV
                     VEAMKMENELRAPRAGRVSEVRVREGAPVDANTVLIVLS"
BASE COUNT          601 a         1209 c         1309 g          629 t
ORIGIN      
        1 gagccgcagc ccgagtccgt acttgtagtt ctcgaccacg cgctccgagc cgggtcctga
       61 aatcgccttg atcgccgaga gctcggggaa cgggttctcc aggtcgttgt agaagaaaaa
      121 cgccgtcacg cggtccagca gcttcgcgtg ggccttgacg atcgtggacg tctgcgtttc
      181 accgagcgtg acgccgtaca gcgcgtttgc cgccgcccag gccaccagcg cgtcatccct
      241 gctgatctgc tcgtacatga tcgtcatccc gaatcgcgcc catccggtct tgggcgcaaa
      301 caggtccgcg ccgacgtaga agccaggctt ggggaccggc gtcttgatcg gcggaccggg
      361 cagaatctcc gctgacgatg gtgtgactcc ccacgtgtag tgcgcgtact cgccgaagac
      421 gcgcacgaac tcctttggct cccagcggcc gaagaccacg agggcgttgt cggtgtgctt
      481 ggagagctgg atcgacgcgg tgtcctcgat cttcgaattg acgtagttct tcctgtacga
      541 cacgccggca aacgagcggc cgaacgtcgc gcgcagcgtg ccgacagccc cgagggcgga
      601 gttcttgtcc tccgccggat cggtgaagtc gaagtacccg tagtcgtgat acgggttcgc
      661 gttgccggtg atggccgcga gctcggctcg aaaccggccc ctgttgtagt acagcgcgag
      721 accgttgtcc gcctggaagt tcacccgctg gatgtgcgtc aggtccttcg ccgtgaacca
      781 caccagatcg ttcacgccca tcccctggtt cacgtagaag cggccgcccc ggaggccgaa
      841 ccggcgcgac gcgctgtgga tgtccaccca gccgatcctg agcgcgccct gctggatgat
      901 cgggtccagc cccgtgtgct gatagtcgtc caccttgtag agcccctcgg gatcagagct
      961 cacgccgcgc gagccgggga agtcgggctg attcgggaag aagtacgtgc gccgatcttc
     1021 cagactggga atgtagggcc gcggtaccac ggcgtcgtcc gcggggttgg cttcgattcg
     1081 atacgagatg tgatcgccga ggtcgccgta ggcggagaag atgacgaacg ccagcttcac
     1141 ctgccgctcg cggcccatcg actcccgcag gctttcgtcc tgcaccgatg cgaggctgag
     1201 atcgaggccg aagtagatcc gttcgccatg ccacgtgagc gacggagtcg aatccggcgg
     1261 cgggtccggc gtggtacctt gcgtgcctgg gtcttgagga ttcgaggcag cggccgacgg
     1321 gaagcacacg agcatcagca caagtacgag ggcgcgcaca ttcatcgcgc gacagagtac
     1381 ctcgaatgcg cgatgctccc aagcctcgga cccgggcgag acctcacgcg agtgctagaa
     1441 tcggtgaggt cttgcgcaag atcctgatcg ccaaccgcgg ggagatcgcg gtccgcgtca
     1501 ttcgcgcgtg ccgtgacctg ggcgttccct cggtggccgt gttctccgac tgcgatcgcg
     1561 cgtcgcgcca cgtccgcatg gccgacgaag ccgtggcgat cggcgcggat cctcccggcg
     1621 agagctacct tcgcatcgat cgcatcctcg acgccgcgcg cgcgagcggc gccgatgcag
     1681 tacatcccgg ctacgggttc ctgtcggaga acgaggcgtt cgccgccgcg tgccgggacg
     1741 ctggtctcgt cttcatcggt cccacgcctg aagcgatcgg ccgcatgggc agcaagacga
     1801 ccgcgaggaa gatcgcctcc gccgcgggag tgccggtcgt gccggggacg gaggaaccgt
     1861 acgggcccga ggcgaccgac gacgaaatgg cgagagcggc aacccggatc ggatacccgg
     1921 tgcttgtcaa ggcggttgcc ggcggcgggg gcaaaggcat gcggctcgtg tcgcgtccag
     1981 aggaactgcg aagcgcgatc cgcacggccc gctccgaggc tgccaccgcg tttggagatt
     2041 cagccgtcta catcgagcgc cggctgacgc ggccgcgcca catcgaggtg cagatccttg
     2101 cagatcgtca cggcgccgtg caggcgttcg tcgagcgcga atgctcgatt cagcggcgtc
     2161 accagaaagt cgtggaggag tcgccctcgc cggtcgtgtc ccgggagctg cgcgaccgat
     2221 tgagcgaggc tgccgtcgcc gtcgcgaagg cagcgggata tacgaacgcg gggacggtgg
     2281 aatttctgct cgacgaggac gggcgttttt acttcctgga gatgaacgcg cggctgcagg
     2341 tcgagcaccc cgtgaccgag atggtcacct ctgttgacct cgtgagctgg caggtcaggc
     2401 ttgctcaggg ggaaccgctc acgctgccgt cgagccacac cctggtaccg ctgggccacg
     2461 ccattgaatg ccgggtctac gcggaggacc ccgatgccgg cttcatgccc tcgccaggcc
     2521 gaatcaccgg gctgcgggtt cccgacggcc ccggcattcg cgatgacagc ggtgtcgaag
     2581 cgggatcgga cgtgccgatc ttctacgacc cgctcctctc gaagctggtt tcgtggggtg
     2641 aagaccggcc ccaggcgacg gcgcgcatgc ggcgggcgct ggacgagtac gaagtgcacg
     2701 ggattcgcac gacgctcccg ttcttcaagt ggctgctggc ccgcccggaa ttcgtcgaag
     2761 cccggtttca caccggcttt ctggacgaac tcttgcaaca gcgcgccgga gagccgtttg
     2821 gagccgccga tccgtccttc gaagaggtcg cagccgtggc tgcgtgcctc gtcgaggcct
     2881 ctcactcccc ccaggcggtt gcccaggcga cccggtcgat tcaggccctc caacggatgg
     2941 gtcccgtcgt ccggggctgg aaggctcgcg cgagggtcga aggacttcgc gagtgaggtt
     3001 cgaggtcgaa acgggcggtc gtcgccgtac catcgacctg agtcgatccg gtgcggactg
     3061 gatcgtacga gtggatgccc ggcagctgtc ggccagcctg gcgcaggcgg gggcgcgctg
     3121 gtccttgctg ctgcgtcagc tcgagtccga gccgctcgcg tcacggcccg ttcacggggg
     3181 acttgccagc agccacgaga ttgcgatcga gccgagggcc aggggcgttc gcgtcgtaca
     3241 cgttgacggc cgcgagatcg ccgtttcgat tctcgatcca cggagccggg cggcccggga
     3301 ccggggcccg gccgacgtgg cagggccccc tgggcgccga agcgcgcagg gcgaaggtgg
     3361 aagcgcaccg cgcgaaggcg gcagcgtcgt ggtctcgccg atgccggggc gcgtggtgaa
     3421 ggtgcttgtg gcgccgggag acgttgttgc cgcacgacag ggcctggtgg tggtcgaggc
     3481 catgaagatg gagaacgagc tccgcgcccc gcgcgcggga cgcgtgagcg aggttcgagt
     3541 tcgcgaggga gcgccggtgg acgccaacac cgtgctgatc gtgctgagct gagcatgccg
     3601 aagtacaacc tgctctcgcg cccgttcgcc cgcaaggcgc gcactttcgt gcggcgcacc
     3661 gtggcgacgt gcgcggtcat cctggccgtg gcgttcttca cgaccatttc gaccgacctt
     3721 ggccccgcgc tcaaggcgca ggccgagg
//
LOCUS       DBNGSH010000327         3419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3252471, whole genome shotgun sequence.
ACCESSION   DBNGSH010000327 DBNGSH010000000
VERSION     DBNGSH010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3252471"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC16CY_07690"
     CDS             <1..200
                     /locus_tag="AC16CY_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HIR3353705.1"
                     /translation="TARIERSVLWDDVVVEDGVMLRECVVTDGVRVPADTSWHGVTLR
                     VATGELAPGERRIDGLAIGEL"
     gene            220..1233
                     /locus_tag="AC16CY_07695"
     CDS             220..1233
                     /locus_tag="AC16CY_07695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR3353706.1"
                     /translation="MRARIDRYLTETGLASRGARVVPLTGDASDRRYVRILLRDEPSQ
                     VLAVYPQAFDVGQLPFANVARLMAAMPVPVPRTLGHSNTLGILALDDLGDVTLQAHLG
                     TASPTEHAALYRQAVTLIDTMQRRGRDLASPDYLPYGIAFDVEKLSWELQFFVKHFLG
                     AYRGAVLSAAEREALDGELMRLAEELAAEPRVLCHRDYHSRNLMLHHGQLYIIDFQDA
                     RMGPDTYDLVSLLRDSYVDVAEREVDELIAFFLALRGVSAGGGAASSEPRAFRRRFDL
                     MAMQRNLKALGTFGFQTTTRGNTVYIQYIPRTLAYVRGNMERDDRFGRLRGLLAAHIE
                     ELR"
     gene            1299..2165
                     /locus_tag="AC16CY_07700"
     CDS             1299..2165
                     /locus_tag="AC16CY_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3353707.1"
                     /translation="MHRFGVSTHLFHDHRLQRDHLAHVAAHGFEAIELFATRSHFDYR
                     SDHAVGDLQQWLADTRLSVHSMHAPIVERMDKGRWVGSFSNASTDDTRRRAAVAETEA
                     ALAVASRIPFSYLVVHLGMPAGEQIPPGDNDREAARRSIEDIAALAARVGVRVALEVI
                     PNPLSSAEALVRLVEEQLEGLDVGVCLDYGHAHLMGDLAEAIEALSGHLWTTHVHDNH
                     GVRDDHLTPYSGAIDWDAAMMETQKIGYDGVLMLEVSDTGDPVRVLQQCVRARERLEK
                     ATDFSDLHVHEN"
     gene            2155..>3419
                     /gene="asnS"
                     /locus_tag="AC16CY_07705"
     CDS             2155..>3419
                     /gene="asnS"
                     /locus_tag="AC16CY_07705"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR3353708.1"
                     /translation="MRTEIQNIARHEGQEVTIHGWLHNRRSSGKIHFLIVRDGTGFIQ
                     AVVSRAAVGDETFARTDHLSQETSLSVTGAVRADARAKGGFEIDVTRLDVHGESHDFP
                     ITPKEHGVDYLMDRRHLWIRTERQAAILRVRHEVINAARDFLNSRGFILCDTPIFTPT
                     ACEGTSTLFPVQYFEDTTAFLTQSGQLYNEANAMALGKVYCFGPTFRAEKSKTRRHLT
                     EFWMVEPEMAYATLEDVIALAEGLLTFIVGRVLETRRAELKLLDRDVTKLEAIKAPFP
                     RLRYDDAAKLLQEKGQPFQHGTDLGGTDETVLSEQFDQPVCVTHYPASIKPFYMKPDP
                     DQPDKALCVDVLAPEGYGEIIGGGQRLDDLDLLLQRIKEHKLPQDAFEWYLDLRRFGT
                     VPHGGFGMGVERCTAWICKLEHVRETIPYP"
BASE COUNT          579 a         1140 c         1140 g          560 t
ORIGIN      
        1 cgaccgctcg aatcgagcgc agcgtcctct gggacgatgt cgtggtcgaa gacggcgtga
       61 tgctgcgcga atgcgtggtc accgacggcg ttcgcgtacc ggccgacacg tcgtggcacg
      121 gcgtgacgct ccgcgtcgcc acaggcgagc tcgcaccggg agaacgacgg atcgacgggc
      181 tggccatcgg agagttgtag ccccgacatg cctgaagacg tgcgagcgcg catcgatcgt
      241 tacctgaccg aaacgggact cgcctcgcgc ggcgcgaggg tggtgccgct gacgggagac
      301 gcgtcggacc gccgttacgt gcgcatcctg ctgcgcgacg agccgtcgca ggtcctggcc
      361 gtttacccgc aggcgttcga cgtcggccag ctccccttcg ccaacgtcgc ccgcctcatg
      421 gccgccatgc cggtcccggt cccgcgtacg ctcgggcact cgaacacgct cggcatcctg
      481 gcgttggacg accttggtga cgtgacgctg caggcgcatc ttggcacggc ttctccaacc
      541 gagcatgcgg cgctgtaccg gcaggcggtc acgctgatcg acacgatgca gcggcggggg
      601 cgcgaccttg cgtcgccgga ctatctgccg tacggcatcg cgttcgacgt cgagaagctt
      661 tcgtgggagc tccagttctt cgtgaagcac tttctcggcg cgtaccgggg ggcggtgctg
      721 tcggccgccg agcgggaggc gctcgacggc gagctgatgc gcctggccga ggagcttgcc
      781 gcggagccgc gcgtgctctg ccatcgcgac tatcacagcc gcaacctgat gctgcaccac
      841 ggtcagctgt acatcatcga ttttcaggac gcgcgaatgg gcccggacac ctacgacctg
      901 gtgtcgttgc tgcgggattc ctatgtggac gtggcggaac gggaagtgga cgagctgatc
      961 gcgttcttcc tcgcgctgcg cggcgtgagc gccggaggcg gcgcggcgtc atcggagccg
     1021 cgtgccttcc ggcgccgctt cgacctgatg gcgatgcagc ggaacctgaa agcgctcggc
     1081 acgttcgggt tccagacgac gacgcgggga aatacggtgt acatccaata catccctcga
     1141 acgctcgcct acgttcgcgg caacatggag cgtgacgatc ggttcgggcg gcttcgcggc
     1201 ctcctggccg cgcacatcga ggagctgcgc tagctgcggc tcgcgaaccc ggctacaatc
     1261 gtcagttggc cgccgcggga acgagcggac cagcaggcat gcaccgattc ggcgtctcga
     1321 ctcacctgtt tcacgatcac cggctgcaac gcgaccacct cgcgcacgtt gccgcgcacg
     1381 gattcgaggc gatcgagctg ttcgccacgc gctcgcattt cgactaccgg agcgatcacg
     1441 ccgtcggcga cctgcagcag tggctggccg acacccggtt gagcgtccat tcgatgcatg
     1501 cgccgatcgt cgagcgcatg gacaaggggc gatgggtcgg ctcgttctcg aatgcgtcca
     1561 ccgacgacac gcgtcgacga gcggctgtag ccgagaccga ggcggcattg gccgtggcct
     1621 cgcgaatccc gttttcctat ctcgtcgtgc acctgggcat gccggcggga gagcagattc
     1681 ctcccgggga caacgaccgc gaggcggcgc ggcgcagcat cgaggacatc gccgccctcg
     1741 cagcccgggt tggcgtgcgc gtcgcgctcg aggtcatccc gaacccgttg tcgagcgccg
     1801 aggcgctggt gcggctcgtc gaggagcagc tcgaggggct cgacgtcggg gtctgcctgg
     1861 attacgggca cgcgcacctg atgggggacc tcgccgaagc gatcgaagcg ctttccgggc
     1921 atctatggac cacgcacgtt cacgacaacc acggcgtgcg cgacgaccat ctgacgccgt
     1981 attccggcgc gatcgactgg gatgcggcga tgatggagac gcagaaaatc gggtacgacg
     2041 gagtgctgat gctcgaggtg tcggatacgg gcgaccccgt gcgggtcctg caacagtgcg
     2101 tccgcgcgcg ggagcggctc gagaaggcga cggacttttc cgacttacac gttcatgaga
     2161 actgagatcc agaacatcgc ccgccatgag ggccaggagg tcaccattca cgggtggctt
     2221 cacaaccggc gctcgagcgg caagatccac ttcctgatcg tccgcgacgg caccggcttc
     2281 atccaggcgg tcgtgtccag ggccgccgtc ggcgacgaga ccttcgctcg cacggaccac
     2341 ctgtcccagg aaacctcgct gtcggtgacg ggcgccgtcc gcgccgacgc gcgggcaaaa
     2401 gggggcttcg agatcgacgt cacgcggctc gacgtgcacg gcgagtcaca cgacttcccc
     2461 atcacgccca aggagcacgg cgtcgattac ctgatggatc ggcgccacct gtggattcgg
     2521 accgagcgcc aggctgcgat cctgcgagtc cgccacgagg tcatcaacgc cgcccgcgac
     2581 ttcctcaact cgcgcggctt catcctgtgc gacacgccga tcttcactcc gacggcctgc
     2641 gagggaacct ccacgctgtt cccggtccag tacttcgagg acacgacggc gttcctcacg
     2701 cagagcggcc agctctacaa cgaagccaac gccatggcgc tcggcaaggt gtactgcttc
     2761 ggcccgacgt tcagggccga gaagtcgaaa acccgccgtc acctcaccga gttctggatg
     2821 gtcgagccgg agatggcgta cgcaacgctc gaggacgtca tcgcgctggc ggaggggctg
     2881 ctcaccttca tcgtcggccg cgtgctcgag acgcggcgcg ccgaactgaa gctgctcgat
     2941 cgggacgtga cgaagctcga ggccatcaag gcgccgttcc cacggctgcg ctacgacgat
     3001 gccgcgaagc tcctccagga gaagggccag ccctttcagc acggcaccga cctcggcggg
     3061 accgatgaaa cggtgctgtc cgaacagttc gatcagcccg tgtgcgtcac tcactatccg
     3121 gcatccatca agccgttcta catgaagccg gatcccgatc agccggacaa ggcgctctgt
     3181 gtagacgtgc tggctcccga aggttacggc gagatcatcg gcggcgggca gcggctcgac
     3241 gatctcgacc tgctccttca gcgcatcaag gaacacaagc tgccgcagga cgccttcgag
     3301 tggtacctcg atctccggcg cttcggcacc gtgccgcacg gcggcttcgg catgggcgtc
     3361 gagcgctgca cggcgtggat ctgcaagctc gagcacgtgc gggagaccat tccgtaccc
//
LOCUS       DBNGSH010000328         3987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2743303, whole genome shotgun sequence.
ACCESSION   DBNGSH010000328 DBNGSH010000000
VERSION     DBNGSH010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2743303"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..873)
                     /locus_tag="AC16CY_07710"
     CDS             complement(31..873)
                     /locus_tag="AC16CY_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353709.1"
                     /translation="MKRLAAAVSLLVLALSGSLFAQQSGERPTTVVIQTPSQGAETTR
                     EQLQGLLEQLPPNVRRVLQLDPSLAERPEYLALYPQLQAFLKVHPEVTRNPAYFFGIA
                     VQDFNRRRSDPMEWFAGILAGIGLFIGFMTVVMILGALVKQAVEYRRWLRQTRMQTDV
                     HTKILDRMQSNEDLLAYIQTPAGKNFLQFTPISTEGAPKVVGAPLSRILWSVQAGVVL
                     AALGLGLRYAQSSVPEEIVPAFTVLGVIVAALGIGAVVSAVVAYLLSARLGLLPPRKE
                     ETHA"
     gene            complement(933..1622)
                     /locus_tag="AC16CY_07715"
     CDS             complement(933..1622)
                     /locus_tag="AC16CY_07715"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3353710.1"
                     /translation="MLMACTPIAQNDSDFGQRRLDMVERQIRQRGVVDPRVLDAMRSV
                     PRERFVPADAASSAYDDSPLSIGFGQTISQPYIVAYMSEALAVRPGHTVLEIGTGSGY
                     QAAVLGRLAREVYTIEIVPELARRASATLRELGIGNVQVREGDGYAGWPERAPFDRIM
                     VTAAPDQIPKPLIEQLAPGGRLVIPVGAQGREQWMTIVDRTGAGTVEKRTIPVQFVPF
                     TRGQGAGGRGQ"
     gene            complement(1659..2657)
                     /locus_tag="AC16CY_07720"
     CDS             complement(1659..2657)
                     /locus_tag="AC16CY_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HIR3353711.1"
                     /translation="MPIRDLLIVGAGPSGLATAIAAKQHGMDYVLVEKGVLVNSIFNF
                     PVHMVFFTTPELLEIGGLPLVTPYDKPTRLEALRYYRRVVDTFGLQISFGEEVCQIDP
                     ESPEGHGVFTVTSRSRGTTRVRHARAIVLAIGYYDRPNMLGVPGEDLPHVSHYYTDAH
                     PYYRQRVVVVGGKNSAAEAALELFRAGAHVTLVHRHAALGESIKYWVRPDIENRIKAG
                     SIAARFESRVVEIRPTEVVVEREGVRDSLPAEGVFLLTGYHPDAELMRRAGVEVHPDT
                     LAPTLDPETCESNVGNLFIAGGASGGRNTGNIFIENGRFHGEKIVKVLAARRLQEA"
     gene            complement(2677..>3987)
                     /locus_tag="AC16CY_07725"
     CDS             complement(2677..>3987)
                     /locus_tag="AC16CY_07725"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3353712.1"
                     /translation="VVVTSAMSGVTNTLVDAAHAAAKGNLDTSLRGKLFERHKAAADA
                     LIPDAAARAAILEQVDRRLDRFEKLCYGLSMVHELTPRLLDAISGTGEMLAAPVVAGA
                     IAARGRASSAVETTEVVVTTDQFGAAEPLMDETRDRMSARIRPLLEKGESPVLTGFIG
                     ATADGVQTTLGRGGSDYSASIVGAALDAGEIWIWTDVDGVLSANPSEVPDARPLAEIS
                     YSEASELAYYGAKVLHYKTILPAFQKRIPVRILNSFNPGHPGTRVSVEGDASMAGVKA
                     VTSIRGVSLIAISGTGMQGIPGIVAKAFDVVAEQQSNVLMISQASSENNMCFVISAAD
                     APRVVRALRTALEFELARGHIEEIVSQPAAVVAAIGDRMRGTPGIAATVFGALGTQRI
                     NVIAISQGSSERNISLVVAEADAAPAVRALHQAFRLDRAAVAAK"
BASE COUNT          660 a         1369 c         1320 g          638 t
ORIGIN      
        1 ccaatccgat ccatgtcggt gagcgtcact tcacgcatgg gtctcttcct tgcgcggcgg
       61 caacagaccc agccgcgccg acaggaggta agcgaccacg gccgagacga cggcgccgat
      121 gccgagagcc gccacgatga cgccgagcac ggtgaaggcc gggacgattt cttcgggcac
      181 gctgctctga gcgtatctca atccaaggcc aagggcggcc agcacgaccc ccgcctgcac
      241 agaccagaga attcgcgaga gcggagcgcc cacgaccttg ggcgcgccct cggtgctgat
      301 cggcgtgaac tggaggaagt tttttccggc gggcgtctgg atataggcca gcaggtcttc
      361 gttcgactgc atgcgatcga ggatcttcgt gtggacatcg gtctgcatgc gcgtctgccg
      421 cagccagcgc cggtactcga ccgcctgctt caccagggcg ccgagaatca tcacgaccgt
      481 catgaagccg atgaacaggc cgatcccggc caggatgccg gcgaaccact ccatcggatc
      541 agaccgacgg cggttgaagt cctggaccgc aatgccgaag aaatatgccg gattccgtgt
      601 gacttccggg tggaccttga gaaaggcctg cagttgcgga tagagcgcca ggtactcggg
      661 ccgttcggcc agcgacgggt cgagctgaag cacccggcga acgttggggg gcagctgctc
      721 gagcaggccc tgaagctgct cccgcgtggt ctcggcaccc tgggacggcg tctgaatcac
      781 gacagtcgtg gggcgttctc cactctgctg ggcgaacaga ctgcccgaaa gagccagaac
      841 caggagcgaa accgccgcag caagcctctt catactccct attacggttc tgccatggac
      901 tggttaacta gcgggtccgg tagcggatgg tcttactgcc ccctgccccc cgccccctgc
      961 cccctggtga aaggcacgaa ctgcacggga atcgtccgct tctcgaccgt gccagccccg
     1021 gtcctgtcca cgatggtcat ccactgttcg cggccttgtg ctccgaccgg aatgaccagc
     1081 cggccccctg gtgccagctg ctcgatgagc ggcttcggga tctgatccgg cgccgcggtc
     1141 accatgatcc gatcgaaggg ggcgcgctcg ggccagcccg cgtagccatc cccctcgcgc
     1201 acctggacat tcccgatgcc gagctcgcgg agcgtcgccg acgctcttct ggcgagctcc
     1261 ggcacgatct cgatggtgta gacctcgcgc gcgagccgtc caaggacggc ggcctgataa
     1321 ccggaccccg ttccgatctc cagcaccgtg tggcccggcc gcacggcgag cgcctcgctc
     1381 atgtacgcca cgatgtaggg ctgcgagatg gtctggccga aaccgatcga cagaggcgag
     1441 tcgtcgtagg cgctcgaggc cgcgtcggcc ggcacgaacc gctcgcgggg caccgatcgc
     1501 atcgcgtcga gcacgcgggg atcgacgacg ccgcgctgcc ggatctggcg ctcgaccatg
     1561 tcgaggcggc gctggccgaa gtcgctgtcg ttctgcgcga tgggcgtaca ggccatcagc
     1621 atcgtcagcg cagccgccca cagggggccg cgcttcactc acgcctcctg cagccgcctg
     1681 gcggccagga ccttcacaat cttctctccg tgaaagcggc cattctcgat gaagatgttg
     1741 ccggtgttcc gcccgcccga cgccccgccg gcgatgaaca ggttccccac gttgctctcg
     1801 caggtctcgg ggtcgagcgt cggcgcaagc gtgtccgggt gcacctcgac gccggcccgc
     1861 cgcatcaact cggcatccgg gtggtagccg gtcaacagga acacaccctc ggccggcagg
     1921 ctgtcgcgca cgccttcgcg ctcgacgacg acctcggtcg gccggatctc gacgacacgc
     1981 gactcgaagc gggccgcgat cgaccccgcc ttgatccggt tctcgatgtc cggccggacc
     2041 cagtacttga tggactcgcc cagcgcggcg tgccggtgca cgagcgtcac gtgggccccg
     2101 gctcgaaaga gctcgagcgc agcttccgca gccgaattct tgccgccgac gaccaccacg
     2161 cgctgccggt agtacggatg ggcgtcggta tagtagtgcg aaacgtgagg cagatcctcg
     2221 ccggggacgc ccagcatgtt cgggcgatcg tagtagccga tggccagcac gatggcgcgc
     2281 gcgtggcgca ctctcgtcgt cccccggctc ctcgacgtca cggtgaacac cccgtgtccc
     2341 tccggcgatt cagggtcgat ctggcacacc tcctcgccaa acgagatctg cagcccgaag
     2401 gtgtcaacga cgcgccggta gtagcggagc gcttcgaggc gagtcggctt gtcgtagggc
     2461 gtcacgagcg ggagcccgcc gatctcgagc agctcgggcg tggtgaagaa caccatgtgc
     2521 accgggaagt tgaagatcga gttgacgagc acgcctttct cgacgagcac gtagtccatc
     2581 ccgtgctgct tggcggcgat ggcggttgcc aggccggacg gcccggcacc gacgatcagc
     2641 agatcgcgaa tcggcatgag aggacgtccg tgcctgtcac ttcgcggcca cggcggcgcg
     2701 gtcgagccgg aatgcctggt gaagcgcccg gaccgcggga gccgcgtcgg cctcggccac
     2761 gacgagcgag atgttgcgct ccgaggagcc ctgcgaaatc gcaatcacgt tgatccgctg
     2821 cgtgcccagc gcgccgaaca ccgtggccgc gattccaggc gtgccgcgca tgcgatcgcc
     2881 gatggcggcc acgacggctg ccggctgtga cacgatctcc tcgatgtgcc cgcgcgccag
     2941 ctcgaactcg agcgcggtcc gcagcgccct gaccacgcgc ggcgcgtcgg cggcgctgat
     3001 gacgaaacac atgttgttct cggaggaggc ctgcgaaatc atcaggacgt tcgactgctg
     3061 ctcggcgacc acgtcgaacg ccttcgcgac gatgcccggg ataccctgca tgcccgtgcc
     3121 gctgatcgcg atcaggctca cgccgcggat cgacgtgacg gccttgacgc cggccattga
     3181 cgcatcgccc tcgacactca cccgcgtgcc gggatgcccg gggttgaagc tgttcaggat
     3241 gcgcacgggg atgcgcttct ggaacgccgg caggatcgtc ttgtagtgca ggaccttggc
     3301 gccgtagtac gccagctccg acgcttcact gtaggagatc tcggccagcg ggcgcgcgtc
     3361 gggcacttcc gacgggttcg ccgagagcac gccgtccacg tcggtccaga tccagatctc
     3421 gcccgcgtcg agcgccgcgc ccacgataga cgccgagtaa tccgacccgc cccggccgag
     3481 cgtcgtctga acgccatcag cggtggcacc gatgaagccg gtcagtaccg ggctctcgcc
     3541 cttttcgagc agcgggcgga tgcgcgcgct catcctgtcg cgcgtctcgt ccatcagagg
     3601 ctcggcggcg ccgaactgat cggtcgtgac gacgacctcg gtggtctcga cggcgctgga
     3661 ggcacggccg cgcgccgcga tcgcaccggc gacgaccggc gcggcgagca tttcgcccgt
     3721 gcccgaaatg gcgtcgagca ggcgcggcgt gagctcgtgc accatcgaga ggccgtagca
     3781 gagcttctcg aagcgatcga ggcgccggtc aacctgttcg aggatcgcgg ccctggcggc
     3841 ggcatccgga atcaacgcgt ccgccgcggc cttgtgccgc tcgaacagct ttcctcgaag
     3901 cgacgtgtcg agattgcctt tcgccgccgc gtgcgcggcg tcgaccaagg tgttggtcac
     3961 gccgctcatc gcggaggtga cgacgac
//
LOCUS       DBNGSH010000329         3424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4271405, whole genome shotgun sequence.
ACCESSION   DBNGSH010000329 DBNGSH010000000
VERSION     DBNGSH010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4271405"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="AC16CY_07730"
     CDS             complement(<1..497)
                     /locus_tag="AC16CY_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HIR3353713.1"
                     /translation="MDPTHLHLLINHVPTVGFGVGVALFVIALAARSNDLKRASLVVF
                     VGVALLSIVTYVTGNAAQEAVMENPDVTESMIVTHEGAAFLALGFMELTGGLAWFALW
                     QFRRTSRLSGGMGGVILILSLVTLVLMTGAANLGGVINHPEIRGAEEVTTRIGPLART
                     VGDFVR"
     gene            687..2519
                     /locus_tag="AC16CY_07735"
     CDS             687..2519
                     /locus_tag="AC16CY_07735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353714.1"
                     /translation="MTRQSIHTLVLVVTLAGGASPAFAQIDLTGMWAPIFDEDRDERI
                     PGPDVGDYAGMPITDALRFRADSWDASILTLPEHQCKPHPSTYGFRGVGNLRISADVD
                     DKTQSIVRLRTHIQWQEQKRDIWMDGRPHPPAYAAHTWQGFSTGSWEGSVLVVRTTHL
                     KAGWIRRNGLFLTDQATMTERFIRHGNYLTHVYMIEDPHYLSEPLVRTNGFRLTPNPA
                     MQPYPCYPTVEVPRARGEVPSFLPGENPFLGEHAKKHNLPPEGVRGGAETALPEFVNA
                     VRGQGAGGRGQGSRPPAPQVRPDPRSPTPDPGTVHALHVQGNVWMLTDGSVNAAVQIG
                     DDGVLVVDTMRDASAEKMIAAIRTLAGDRPIRWIVNTHMDSDHTGGNAKVSEAGESII
                     AGNFTGQVSRSAGEFAQILAHENVDARMVKAQPALPQAAMPTDTFFADRFDMFFNGEA
                     IQVLHQKNAHSDGDVLVFFRKSDVLVAGDTFVTTTFPRIDRREGGSLDGILASLNQIL
                     DITVPKDKQEGGTYVIPGHGRLADEADVVEYRDMVTIIGDRLRNAIAKSMTLEQVKAS
                     KLLLDYEGRFGGSEGVWTKDAFIEAAYGSLSVERRPQPPRASSK"
     gene            2604..3374
                     /locus_tag="AC16CY_07740"
     CDS             2604..3374
                     /locus_tag="AC16CY_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353715.1"
                     /translation="MRRCVWAVVAAIVIVNSASSLSAQRGRDGGPAQPPLSPQASAPL
                     DLTGNWVSVVTEDWRWRMVTPPKGDFASVPLNPEGQKVGNAWDPSTDGSCLAYGAAGL
                     MRIPTRLRITWENERTLKVETDAGVQTRRFLFGESQKPAERSPQGYSVAEWEVPAAGR
                     GGPGAGRGGPPPARRGSLKVTTTMLSAGWLRKNGAPYSENASLTEYYDRFAAPNGEEW
                     FAVTTIVSDPRYLNTDFVTSSHFKKEADGSKWAPKPCK"
BASE COUNT          632 a         1117 c         1136 g          539 t
ORIGIN      
        1 cgcacgaaat cgccaaccgt gcgcgccagc ggcccgatcc tcgtcgtcac ttcctcggcg
       61 ccgcggattt ccgggtgatt gatcactcca ccgagattgg cggcgcccgt catgagcacg
      121 agcgtcacga gggacaggat gagaatgacg ccgcccatcc cacccgagag tctcgacgtc
      181 cgccggaact gccagagcgc gaaccaggcc aggccgcccg tcagttccat gaacccgagc
      241 gccaggaagg cggcgccctc gtgcgtcacg atcatcgatt cggtgacgtc ggggttctcc
      301 atcaccgctt cctgggcggc gttgccggtc acgtaggtca caatcgacag cagcgccacg
      361 ccgacgaaga cgaccaggct tgcccgcttg aggtcgttgc tgcgggcggc cagcgcgatg
      421 acgaaaagcg cgacgccgac gccgaagccg accgtcggca cgtggttgat caggagatga
      481 agatgtgtcg gatccatggt tcacacgtcg ctagcgacag cgtacgagtt cactgttgtc
      541 ccgggagggt ggtgtcgaga tcaggaccgg gtcgacgggg cactcgaggc ttggcaaacc
      601 gacgggcgag tctattgccg attcgacaaa aagtccaccc ggcactaaca tgcggggcgc
      661 acgcagccac ttttccgagg gctttcatga cgcggcaatc cattcacacg ctggtgctgg
      721 tggtcaccct cgcgggtggc gcgagccccg ccttcgcgca aatcgacctg accgggatgt
      781 gggcgccgat cttcgacgag gatcgagacg aaagaatccc cggtcccgac gtgggcgatt
      841 acgcgggcat gccgatcacc gacgcgctcc ggttccgggc agacagttgg gatgcctcga
      901 tcctcactct tcccgagcat cagtgcaagc cgcacccgtc cacctacggg ttccgcggtg
      961 tgggaaatct ccgcatcagc gccgacgtcg atgacaagac ccagtcgatc gtccggctcc
     1021 gcacgcacat tcagtggcag gagcagaagc gggacatctg gatggacggg cggccgcacc
     1081 cgccggcgta cgcggcgcac acgtggcagg gattctcgac cggcagctgg gaaggcagcg
     1141 tcctcgtcgt gcggacgact cacctcaagg cagggtggat tcgccgcaac ggcctgttcc
     1201 tgaccgacca ggcgacgatg accgaacggt tcatcaggca cgggaactat ctgacccacg
     1261 tgtacatgat cgaggatccc cactacctgt cagagccgct cgtcaggacc aacggcttcc
     1321 gcctgacgcc gaacccggcg atgcagccgt atccgtgcta cccgaccgtc gaagtgccgc
     1381 gcgcaagagg cgaagtaccg agcttcctgc cgggcgagaa ccccttcctc ggcgagcacg
     1441 ccaagaagca caacctgccg cccgagggcg tgcgtggggg tgccgagact gcgttgcctg
     1501 agttcgtgaa cgccgtcagg gggcaggggg cagggggcag ggggcaggga tcaagacctc
     1561 ctgctccaca ggttcggcct gatccccgat ccccaactcc cgatcccggt accgttcacg
     1621 cccttcacgt ccagggcaac gtgtggatgc tgaccgacgg ctccgtcaac gccgccgtgc
     1681 aaatcggcga cgacggcgtg ctggtggtgg acaccatgag ggacgcgtcc gccgagaaga
     1741 tgattgccgc catccgcacg ctggccggcg acaggccgat ccggtggatc gtcaacacgc
     1801 acatggattc cgatcacacc ggcgggaacg cgaaggtgtc ggaggcaggc gagtcgatca
     1861 tcgccggcaa cttcacgggc caggtgagcc gcagtgccgg agaattcgcc cagatcctcg
     1921 cgcacgagaa cgtcgacgcg cgcatggtca aggcgcagcc cgccctgccg caggcggcca
     1981 tgcccaccga cacgttcttc gccgaccggt tcgacatgtt cttcaacggc gaggcgatcc
     2041 aggtgctcca ccagaagaac gcccactccg acggcgatgt tctcgtcttc ttccggaagt
     2101 ccgatgtcct ggtcgccgga gatacgttcg tgacgacgac gttcccgcgg atcgaccggc
     2161 gcgagggcgg cagcctcgac gggatactgg cgtcgctcaa tcagatcctc gatatcaccg
     2221 tgccgaagga caagcaggaa ggcggcacgt acgtgattcc cggccacggc cgcctcgccg
     2281 acgaggccga cgtcgtcgag taccgagaca tggtcaccat catcggcgac cgtctgcgga
     2341 acgctatcgc gaagagcatg acgctcgaac aggtgaaggc gtcgaagctg cttctcgact
     2401 acgaagggcg cttcggcggg tcagagggcg tgtggacgaa ggatgcgttc atcgaagccg
     2461 cgtacgggag cctcagcgta gagcggaggc ctcagccccc gcgtgcaagt tcgaagtaga
     2521 gcgggggctt caggccccgc gtgcaagctc gacgtagagc gggggcttca gcccccgcgt
     2581 gcaagttcta agtggagacg tacgtgagaa ggtgcgtttg ggctgtagtc gcggccatcg
     2641 ttatcgtcaa ctccgcgagt tcactctccg cgcagcgcgg gcgtgacggt gggcctgcgc
     2701 agccgccgct ctcgccgcag gcgtcggccc ccctcgatct caccggcaac tgggtgtctg
     2761 tcgtcaccga ggactggcgc tggcgcatgg tcacgccgcc caagggtgac ttcgcgagcg
     2821 tgcccttgaa cccggaaggg cagaaggttg gcaacgcgtg ggacccgtcc accgacggtt
     2881 cctgtctcgc gtatggcgcg gccgggctga tgagaatccc gacgcggctc cgcatcacct
     2941 gggagaacga gcgcacgctg aaggtcgaga ccgatgccgg cgtgcagacg aggcgtttcc
     3001 tcttcggcga gagccagaag cctgccgagc gctcgccgca ggggtattcg gtcgccgagt
     3061 gggaggtccc cgccgcgggc cgcggtggtc ccggcgccgg ccgcggcggc ccgccgcctg
     3121 cacgtcgcgg cagcctgaag gtgacgacga cgatgctgag cgcggggtgg ctgcggaaga
     3181 acggcgcgcc ctacagcgag aacgcgtcgc tgacggagta ctacgacagg ttcgcggcgc
     3241 ccaacggcga ggagtggttt gccgtgacca caatcgtgtc agaccccagg tacctgaaca
     3301 ccgacttcgt cacgagctcg catttcaaga aggaagccga cggatcgaag tgggcgccca
     3361 agccctgtaa gtgacatgtc gttcacactt ctcggttctc gcttctcggt tcgtgttcac
     3421 gttc
//
LOCUS       DBNGSH010000330         9125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_706229, whole genome shotgun sequence.
ACCESSION   DBNGSH010000330 DBNGSH010000000
VERSION     DBNGSH010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_706229"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="AC16CY_07745"
     CDS             complement(<1..658)
                     /locus_tag="AC16CY_07745"
                     /inference="COORDINATES: protein motif:HMM:NF025086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR3353716.1"
                     /translation="MSRLRAGIGVVLALVVVSGAVSLAQRFGVFEGAGAGIRVPPKDF
                     NDSRFTVCKWMFRSDRSEPSGIGWSTDYPFGEINLLTRISEFTNVRVSQTGGDLNYWV
                     VRLTDDQLFECPMLLGSDVGTAALSNTEVQRLREYLLKGGFLWVDDFWGTPAWDQWAG
                     LITRVLPEFPIVDVAPGDAIRHALFRIDEVKQVPSIFAWGGPGSDPRERGSDSPHANF
                     R"
     gene            complement(817..2058)
                     /locus_tag="AC16CY_07750"
     CDS             complement(817..2058)
                     /locus_tag="AC16CY_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353717.1"
                     /translation="MHTRLVLVSVAVVGLALTAAAAAQEREAESPSLTVRTSVKATGL
                     LSRSPNAPELFPERTAGESMLRLRVEPEIRTIGSTTFSLAYEQRLRYASAPMVGIAAL
                     GILPSELATPFRIEPLVWRVSESANGVWRHEIDRANAQVHLRRADLTIGRQSIGWGRG
                     VMFTSVDLFAPFSPLEVDREWRPGVDAVRADVKLTDRSSIDLVGAFGDTLDRSAFAAR
                     ARGYAGTIDVEVVGGRRGQDLFGGVTSSAAVGDAEVHGEAAAFRVPSGIDHDVVWKVV
                     VGGAYRFPIGSGILAYAEYHYSGFGAAHPEAILVLLTTPSFLERFVRGDMQILSRHAV
                     GVTGSYEVSPEITYSGQWLHNPGDRSGIVAPSMTYTFNDAVSLLGGVYVPYGRPPEGG
                     LLRSEYGASPQSALLQLRLYF"
     gene            complement(2062..2808)
                     /locus_tag="AC16CY_07755"
     CDS             complement(2062..2808)
                     /locus_tag="AC16CY_07755"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353718.1"
                     /translation="MNEPILTARGVTKVFQLGRQQVRALRGVDLEVRAGEFLALVGPS
                     GSGKTTLLNLVGALDVPTTGDLRVLGHRIATLSRRQRADLRLRSIGFVFQAFNLVPVL
                     TALENVEFVLELQGLEHRRDRARAVLQELGLADLADRRPSELSGGQQQRVAVARAIAA
                     RPALVLADEPTANLDGENAEILMHMMRDLRERHRMTFIFSTHDPRVVAHAVRVVTLVD
                     GRVARDEVTPGEEPHEIIPGIGKAPATMSA"
     gene            complement(2808..4037)
                     /locus_tag="AC16CY_07760"
     CDS             complement(2808..4037)
                     /locus_tag="AC16CY_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353719.1"
                     /translation="MGTAWTYALRSLRRNARRTTLSIVGIGIGCALALFMESLNRGRD
                     ELFARMGAYGGAGHVRIVPDGWRERRDPRLRLGNLQADVEAARHLSGVAAVTVRARAQ
                     ALVAMGTHVVPVEIVGVDPGTEPGTNRFARNVSQGRYLQSLDRGAVVIGQAIADRLLA
                     GLEDEILATAVGKNGDIESTLLRIVGIVHTGSDEIDATICHVVLADVERLTGLSGAGE
                     VTLVLEDWERADLVKAALSHAVHAGDEVLTWGEINPEFKGHLEQDNAVSRLVSGIILL
                     IVMLGVASAQLAAVLERRREFAVLSALGVHSRTIVAVLLQEALVIGIASAALGLLLGL
                     PFVWALATRGIDLSRYMGASYSFQGVLIEPVIYGDLGAWTAWYVLTVAVVVTVIASLY
                     PAWFASRSNPADALRVA"
     gene            complement(4037..5326)
                     /locus_tag="AC16CY_07765"
     CDS             complement(4037..5326)
                     /locus_tag="AC16CY_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353720.1"
                     /translation="MTRSVSRDSNVPASEVAMNWTGSTMRMAWRNLWRNPRRTALALA
                     AIGLSVTLVLAYTSILRAYGQWIVETITGPMLGHVQVHAPLWRKDRLMERTLRDVDAT
                     LAELRRDPDVASATARVYAPALAAREEEGFAVVVMGIASGAESGPARLVTDVVEPLRE
                     REALIGRQLAELMNVRMGDEVAVVGQGVDGSLANDLFTVKGVVTTSVDLVNRQGILIE
                     LSDAQALFAMPDEAHEIVIHARRPEMASALSRRLAGASGFADAEVLDWQTLAPEMVTL
                     VRITDLAGLLVLLLVFIAAAAGVANTMVMATFERTREFGMLLALGTHPSRLVRLVIAE
                     SIALGVVGALTGSLIGIVLVVITHRTGIDYSWLAGGGPSELSFAGLRFSLRFYPALSA
                     VDVARAIGAVCLTSLLAATWPALRASRLQPVQALRGT"
     gene            complement(5283..6182)
                     /locus_tag="AC16CY_07770"
     CDS             complement(5283..6182)
                     /locus_tag="AC16CY_07770"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR3353721.1"
                     /translation="MTRLAPAGIGAVSGAAQALRRHPGVRAVVLQQVTSSRPICEQPM
                     RSSLGLVIAAALAGMCSGGAVSLAAGPDRPDVDALLQHLDDLYRSKSSIARIQIDVTG
                     TRSTRSMRLRAWTRGEEEVLVLIEAPPREAGTATLRVGNNLWNYLPKIARTIRVPPAA
                     MLGSWMGTDFTNDDLVKESSLRKDFTARLDRRSDAPPGWWLTLDVKPDVVGRWARIDL
                     LVSDERLPIEERHYDRKGRLARTMHFDDVKVLGGRRLPARIVLVPVDGVGQRTEMRYL
                     DAEFDVPLSDDTFSLSRLERTRQ"
     gene            6348..6821
                     /locus_tag="AC16CY_07775"
     CDS             6348..6821
                     /locus_tag="AC16CY_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HIR3353722.1"
                     /translation="MNVLNRKWWSRIGVGLLGTFLAIQLVPYGRDHTNPPVTGEPTWD
                     APETRALAEQACFDCHSNETRWPAYASIAPASWLVQHDVNEGRAVLNFSEWLRPQEEA
                     KEASETILEGEMPPAAYTLVHAHARLNAADRDRLAQGLAKTLGVALEQEARERHR"
     gene            6983..8758
                     /locus_tag="AC16CY_07780"
     CDS             6983..8758
                     /locus_tag="AC16CY_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3353723.1"
                     /translation="MALRWGSDLSPAARALPLYVVLVAGGVPLVLSLTLKLVRREFGS
                     DLLAGISIVAAALLEEYLAGALVVLMLSGGAALEAFALGRASSVLQALARRMPLVAHR
                     RINRQVAEIQLGDVRVGDELIVYPHDICPVDGVVIEGHGVMDESYLTGEPFMMPKAPG
                     SEVFSGAINGENALTLRASRLAVDSRYARIMSVMRDSEQQRPQLRRLGDQLGAWYTPI
                     ALGLAAGAWTWSGDPVRFLAVLVVATPCPLLIGIPVAIIGAISLAAKRSIIIRDPAVL
                     ERVDRCRTIVLDKTGTLTYGEPALTERFTAPGMDDDLVLQVTASLERYSKHPLAKPIL
                     QAARQAHLALIDANEVRERPGEGLEGHVEGRHVRVIGRRQAETMGHGDALPPVSTGLE
                     CVILLDGRYAATYRFHDAPRADSRSFVTHLRPRHQLERILLVSGDRESEVRYLADRVG
                     IEEVYAGQTPEQKVAITRQETERAPTLFIGDGINDAPALVTATVGLAFGHQSEITTEA
                     AGAVIMDTSLKRVDELFHLARRMRRIALQSAVGGMVLSVVGMFIAFGGWLPPVGGAVF
                     QEVIDLAAVLNALRAAVPGGELSDY"
     gene            8846..>9125
                     /locus_tag="AC16CY_07785"
     CDS             8846..>9125
                     /locus_tag="AC16CY_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016349764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3353724.1"
                     /translation="MRAGSIPPGEALLIHSLVTPTEQGIASRVLARTAGGNLTLFAFD
                     AGQELSEHTAPFDAMVLVLEGTLALTIGGAVVRATPGTIVRMPSHIPHA"
BASE COUNT         1636 a         3088 c         2899 g         1502 t
ORIGIN      
        1 cgcggaagtt cgcatgggga ctgtcgctcc cccgctcccg cgggtcgctg ccgggaccgc
       61 cccacgcgaa gatgctgggc acctgcttga cctcgtcgat cctgaacagc gcgtgccgga
      121 tcgcatcacc tggcgcgacg tccacaatcg ggaactccgg cagcacacgc gtgatcaggc
      181 cggcccactg atcccaggcc ggcgtgcccc agaagtcgtc cacccagagg aagccgccct
      241 tcagcaggta ctcgcgcagg cgctgcacct cggtattcga cagcgccgcg gtgccgacgt
      301 ccgacccaag cagcatcggg cactcgaaca actgatcgtc ggtgagccgc acgacccagt
      361 agttgagatc gccgccggtc tggctgacgc gcacgttggt gaattccgag attcgcgtga
      421 gaaggttgat ctcgccaaac gggtagtcgg tgctccagcc aattccgctt ggttccgacc
      481 gatcgctgcg gaacatccac ttgcagacgg tgaaccggct gtcgttgaag tctttgggcg
      541 gaacccgaat gccggcgccg gcgccctcga acacgccgaa ccgttgtgcg agcgacaccg
      601 ctccgctgac gacaacgagg gcgagcacga caccgatgcc agcacgcagg cgcgacatgg
      661 atacccgtag tgtaagccga tgcgggctgg atctacccga acagacattc gagatgcgtc
      721 acttggctcc gatcccttct cgacaagggc acgcgcaacg catcagggag aggcatcaag
      781 tcacgtggag acgctccgcg tcaccgtagt cgaccgtcag aagtacagac gcagttgcag
      841 cagcgcggac tgcggcgagg ctccgtattc gctgcggagc agcccgccct ctggcgggcg
      901 gccatacggg acgtaaacgc cgcccagaag cgacacggcg tcgttgaacg tgtacgtcat
      961 cgagggcgcg acaatacccg atcgatctcc tgggttgtgt agccattgcc cggaatacgt
     1021 gatctccggg gacacttcgt aggagcctgt cactccgact gcgtgcctgg aaagaatctg
     1081 catgtcgccg cggacaaacc gttcgaggaa cgagggcgtc gtcagcagca caaggattgc
     1141 ttctgggtgg gcggcaccga aaccggaata gtggtactcc gcgtacgcca ggatgccaga
     1201 cccaatgggg aaacgatacg cccccccaac cacgactttc cacacgacgt cgtgatctat
     1261 accggaggga acgcgaaacg ccgccgcctc gccatgcact tcggcgtccc cgaccgcggc
     1321 ggacgatgtc acccctccaa acaggtcctg cccacggcga ccgcctacga cttccacgtc
     1381 aatcgtgccg gcatagcctc tcgctcgtgc cgcaaacgcc gaccgatcca gcgtgtcccc
     1441 gaacgcgccg acgagatcga tggatgatcg gtcggtgagc ttgacgtccg cccggacggc
     1501 atccactccc gggcgccatt cgcgatcgac ctccagcggt gagaacggcg cgaacaggtc
     1561 gacggaggta aacatcacgc ctcggcccca gccgatcgac tgtctcccaa tcgtcaggtc
     1621 ggcgcgtcgc aggtgtacct gtgcgttcgc gcggtcgatc tcatgccgcc agacaccatt
     1681 cgccgattcg ctcacgcgcc agacgagcgg ctcaatccgg aacggcgtcg ccagctcgga
     1741 cggaagtatc ccaagcgcgg cgattccgac catcggtgct gacgcgtacc gcagccgttg
     1801 ctcgtacgcg aggctgaacg tggtgctgcc gattgttcgg atctctggct cgacccgcag
     1861 tcgaagcatg gattcgcctg cggtccgttc agggaagagt tcgggtgcgt tcggcgatcg
     1921 cgaaaggaga ccggtcgctt tgactgacgt gcgaacggtc agcgacggcg attccgcctc
     1981 tctctcctgt gcggcggcgg cggccgtcag cgcgagaccg acaactgcga cggacacgag
     2041 gaccaggcgc gtatgcatag gtcacgcact catggttgcc ggtgctttgc cgatccccgg
     2101 aataatctcg tgcggctcct ctcccggggt gacttcgtcg cgggcgacgc ggccgtcgac
     2161 cagggtcacg actcggaccg catgcgccac cacgcgtgga tcgtgcgtgg agaagatgaa
     2221 cgtcatgcga tgccgttccc tgaggtcgcg catcatgtgc atgaggattt cagcgttctc
     2281 gccgtcgaga ttcgcggttg gttcatcggc gaggaccagt gcggggcgtg cagcgatcgc
     2341 tcgcgccacc gcgacacgct gctgttgacc tcccgagagc tcgctgggcc ggcggtcagc
     2401 cagatccgcc agtccgagtt cctgaagcac ggcccgggcg cggtcgcgac gatgctcgag
     2461 cccctgcagt tccagcacga attcgacatt ctcgagcgcc gtcaacacgg gcacgagatt
     2521 gaacgcctgg aacacgaacc cgatcgaccg caggcgcaga tctgcgcgct gccgtcgaga
     2581 gagcgtcgcg atccgatgac cgagcactcg cagatcgcca gtcgtaggca cgtccaacgc
     2641 ccccacgagg ttcaggagcg tggtctttcc gctccccgac ggaccgacca gagcgaggaa
     2701 ctcgcccgct cgcacctcga ggtcgactcc tctcagcgcc cgcacctgct gccggccaag
     2761 ttgaaagacc ttcgtcacgc cgcgtgccgt cagaatcggt tcgttcatca cgccacccgt
     2821 aacgcgtcgg cgggattgct cctcgacgca aaccatgccg gatacagtga ggcgatcacg
     2881 gtcacgacca ccgccacggt gagtacgtac caggcggtcc acgccccgag gtcaccgtag
     2941 atgaccggct caatgagcac cccctggaag ctgtaactcg cgcccatata ccgactcaga
     3001 tcgatgccgc gcgtggccag ggcccagacg aagggcagcc ccaacagcaa gcccagggcg
     3061 gcacttgcga tcccgatcac gagtgcctct tgcagcaata ccgcgacgat cgtgcggctg
     3121 tgcacaccca gcgccgagag cacggcgaac tcccgtcggc gttcgagcac ggcggcgagc
     3181 tgcgcgctgg caacgccgag catgacgatc agcaggatga tgccgctcac gagccgggag
     3241 acggcgttgt cctgctccag gtgacctttg aactccgggt tgatctcgcc ccacgtcagg
     3301 acctcgtcgc cggcgtgcac cgcgtgactc agcgccgcct tgaccaggtc ggcgcgttcc
     3361 caatcttcca ggacgagcgt cacctctccg gcaccgctca acccggtgag ccgttccaca
     3421 tccgcgagca cgacatggca gatcgtcgcg tcgatctcgt cgctacccgt gtgcacgatg
     3481 ccgacgattc tcagcagtgt gctctcgata tctccgttct tcccgacggc ggtggcgagg
     3541 atctcgtctt cgagtccagc gagcagtcga tcggcgatcg cttgaccgat cacaacggcg
     3601 cccctgtcca acgactggag gtaccgaccc tggctcacat tccgcgcgaa tcggttcgtc
     3661 cccggctccg tgcctgggtc gacgccgacg atctcgaccg ggacgacatg ggttcccatg
     3721 gcgaccagcg cctgcgcccg cgctcgtacg gtgaccgcgg ccacgcctga gaggtgtcga
     3781 gccgcctcga cgtcggcctg cagattcccc agacgcagcc gcgggtcccg acgctcacgc
     3841 catccgtcgg gcacgatacg aacatgcccg gccccgccgt acgcgcccat gcgcgcgaac
     3901 agctcgtcgc gccccctgtt caggctctcc atgaagagcg ccagcgcgca gccgatcccg
     3961 atgcccacga tcgagagggt cgttcgccgc gcattgcgcc ggagactccg aagcgcgtag
     4021 gtccacgcgg tgcccatcac gtgcccctca acgcctgtac cggctgcagc cggctcgcgc
     4081 gaagcgccgg ccatgtcgcg gccagcagtg aagtcaggca gacagcgccg atcgctcggg
     4141 cgacgtccac cgcgctgagc gccggataga accggagcga aaaccgcagt ccggcgaaag
     4201 acagctcgct ggggccgcca cccgccagcc agctgtagtc gattccggtt cggtgtgtaa
     4261 tcacgaccag cacgatgccg atcaacgacc ccgtcaacgc accaaccact ccgagcgcga
     4321 tcgattcggc gatgaccagc ctcacgaggc ggctcgggtg tgtgccgagg gccagcagca
     4381 ttccgaattc gcgcgtccgt tcaaaggtgg ccatcaccat cgtgttcgcg acacctgcgg
     4441 cggcggcaat gaagaccagc aggagcacga gcaggccggc gaggtccgtg atgcgcacga
     4501 gtgtcaccat ctccggcgcc agcgtctgcc aatcgagcac ttcagcatca gcaaacccgc
     4561 tcgcgcccgc gagacgccgc gacagggcgg acgccatttc aggtcgtcgc gcgtggatca
     4621 cgatctcgtg cgcctcgtcg ggcatggcga agagcgcctg cgcatccgac aactcgatga
     4681 ggatgccctg ccgattcacc agatcgacgg atgtcgtcac gacgcctttc accgtgaaca
     4741 ggtcgttcgc gagcgagccg tcaacgcctt gccccaccac agcgacttca tcgcccatac
     4801 gaacattcat cagctcggcg agctgacggc cgatcagcgc ttcccgctct cggagtggtt
     4861 cgacgacgtc cgtcaccagc cgcgccggac cggattccgc gccgctggcg atgcccatga
     4921 cgacgacggc gaagccttcc tcctcgcgcg ccgccagcgc cggggcgtag acgcgagccg
     4981 tggccgaggc cacatcggga tcgcggcgga gctcggcgag cgtcgcatcg acgtctcgca
     5041 gcgtccgctc catgagccgg tccttccgcc acagcggcgc atgcacttgg acgtgcccca
     5101 gcatcgggcc ggtaatggtt tcgacgatcc actgaccgta ggcgcgcaaa atcgaggtgt
     5161 acgcgaggac gagggtcacc gagagaccga tcgccgccag ggccagggcg gtccgacgag
     5221 ggttgcgcca gaggttccgc cacgccatgc gcatcgtgct gccggtccag ttcatcgcga
     5281 cctcactggc gggtacgttc gagtcgcgag agactgaacg tgtcatcgga cagcggaacg
     5341 tcgaactcgg cgtccagata ccgcatctcg gtacgctgac caaccccgtc gacgggcacg
     5401 agcacaatgc gggccggcaa tcgccgcccg ccgagcacct tcacatcgtc gaagtgcatg
     5461 gtgcgcgcaa ggcggccctt gcggtcgtag tgacgctcct cgatcgggag tcgctcgtcg
     5521 gagacaagca ggtcgatgcg ggcccaccgg ccgacgacgt ccggtttgac gtcgagcgtg
     5581 agccaccaac ccggcggcgc atcggatcgg cgatcgagcc gggcagtgaa gtccttccgc
     5641 aacgaagact ccttcaccaa gtcgtcgttc gtgaagtcgg ttcccatcca ggatccgagc
     5701 atcgccgcag gcgggacgcg gatcgtacgg gcgatctttg gcaggtagtt ccacaggttg
     5761 ttgcccacgc ggagagtcgc cgtacccgcc tcacggggcg gcgcctcgat caggacgaga
     5821 acctcctctt cgccgcgggt ccaggcccgc aggcgcatgc tgcgggtgct tcgagtgccc
     5881 gtgacgtcga tctgaatgcg cgcgatgctg ctcttcgagc gatacaggtc gtccagatgc
     5941 tggaggagcg cgtcaacgtc tggccggtca ggcccggctg ccagggagac agcaccgccc
     6001 gaacacatgc ccgccaacgc ggcggcaatt acgagcccaa gagaggaacg catcggctgc
     6061 tcgcaaatcg gtcgagacga tgtcacctgc tgcaagacca cggccctcac ccctggatgg
     6121 cggcgcagcg cctgagccgc gcccgaaacg gcgccgattc cagccggcgc caagcgtgtc
     6181 atcgcggaga attccgcacc gacccttaaa tctccgcacg gctctatacc ggcgctcggc
     6241 accaccaggg cgccgtcatc cggtagatgc tccaatcgca cagagaacat cggcacgcca
     6301 cctgcactct catctgcggc ggttcgacgg aatcagtgga ggtctctatg aacgtcttga
     6361 atcggaagtg gtggtcccgg atcggtgtcg ggcttctcgg gacgttcctg gctatccaat
     6421 tggtcccgta cggccgcgac cacaccaacc ccccggtcac cggcgagccg acctgggatg
     6481 cgccggagac gcgcgcgctg gccgagcagg cgtgcttcga ttgccacagc aatgaaacca
     6541 ggtggcctgc ctacgcgagc atcgcgcccg cctcgtggct cgtgcagcac gacgtgaacg
     6601 aaggccgcgc cgtcctgaac ttctccgagt ggctgcggcc tcaggaagag gcgaaggagg
     6661 cctctgaaac aatcctggag ggcgagatgc cgcctgcggc gtacacgttg gtccatgcac
     6721 acgctcgcct gaatgcggcg gaccgcgatc gactcgcgca gggacttgcg aagacgctgg
     6781 gcgtggctct ggaacaggag gcgcgcgaac gccaccgcta gtccgttgcg ttggcatgtc
     6841 gtgagtgtct gcgatgagcg tggcgaccca atcgatttcc gccgcggcag ggtccgggac
     6901 tggcacacgt gtgtggtggc cgagctccgc tgcgcgcgca tcgggcatcg cggcattagc
     6961 catcgccgga attctcacgc acatggcgtt gcgctggggc tctgatctat cccctgcggc
     7021 gagggcgctc ccgctctacg tcgtgctcgt cgcgggcggc gtgcctctcg tgctgtccct
     7081 cacgctcaag ctcgttcgcc gcgagttcgg ctccgacctg ctggcgggaa tctcgattgt
     7141 cgcagcagct ctcctcgagg agtacctggc aggcgctctg gtggtgttga tgctctctgg
     7201 tggcgcagcg ctcgaggcgt tcgcgctcgg gcgtgcgtcc tccgtcctgc aggcgctcgc
     7261 tcgacgcatg ccgctcgtcg cacatcgacg gatcaaccgc caggtcgccg aaatccaatt
     7321 aggagacgtg cgcgtcggcg acgaattgat cgtgtacccg cacgacatct gtcctgttga
     7381 cggcgtggtc atcgaaggtc acggtgtgat ggacgagtcc tatctcaccg gcgagccgtt
     7441 catgatgccg aaggcacccg ggtccgaggt gttctcgggc gcgatcaacg gtgagaatgc
     7501 gttgaccctg cgggcgagcc ggttggcggt cgattcccgc tatgcccgca tcatgagtgt
     7561 catgcgggac tcggagcagc aacggccgca actccgccgg ctcggtgacc aactgggcgc
     7621 gtggtatacg ccgatcgcct tggggctcgc cgccggcgca tggacgtgga gtggcgatcc
     7681 cgtacgcttc ctggccgtcc tcgtggttgc gacgccatgt ccgctgctga ttggcattcc
     7741 tgtcgcgatc atcggcgcca tctcgcttgc cgccaagcgc agcatcatca ttcgtgaccc
     7801 ggcggttctc gaacgcgtcg accgatgccg cacgatcgtc ctcgacaaga ctgggacgtt
     7861 gacctacggc gagcccgccc tgaccgaacg gttcaccgca ccgggcatgg acgatgacct
     7921 ggtgttgcag gtaacggcga gccttgagcg ctactcgaag cacccgctgg cgaagcccat
     7981 tctccaagcc gcgaggcagg cacatctcgc gctgattgac gccaacgagg tgcgcgagcg
     8041 accgggtgaa ggcctcgagg gccacgttga gggccggcat gtgcgggtca tcggtcgccg
     8101 ccaggccgag accatggggc acggcgacgc cctcccgccc gtttcgacag gcctcgaatg
     8161 cgtgatcctg cttgacggcc gatacgcggc cacctaccgg ttccatgatg cgccgcgcgc
     8221 agacagccga tcctttgtca cccatctccg acctcggcac caactcgagc gaatcctgct
     8281 cgtctcaggc gatcgcgaga gcgaggtgcg ctatctggct gatcgggtcg gcatcgagga
     8341 ggtctacgca ggacagacgc cagagcaaaa ggtcgcgatc actcgccagg aaaccgaacg
     8401 cgcgccgacc ttgttcatcg gcgatggcat caacgatgcc ccggcgctgg tcacggcaac
     8461 cgtcggtctt gccttcggcc atcagagcga gatcaccacc gaagcggcgg gggccgtcat
     8521 catggacacg tcactcaagc gtgtcgacga actgtttcac ctcgcgcgcc ggatgcgacg
     8581 catcgcgctc caaagcgcag tcggcgggat ggtactgagc gtcgtcggga tgttcatcgc
     8641 cttcggcgga tggctgccgc ctgtcggcgg cgcggtgttc caggaggtga ttgatcttgc
     8701 ggcggtactc aacgcgttgc gagccgccgt tccgggaggc gaactgagcg actattgacg
     8761 caggcgtctg ccacgcgggt cttgggttcg gacgatgagc gatgattcac gatcgctgaa
     8821 tccacaagag gagcacgcga tgtcgatgag ggccggatcg attccacccg gcgaggctct
     8881 gctgatccac tcgctggtca caccgaccga acaggggatc gccagccgag tgctggcgag
     8941 aacggcaggc gggaatctca cgttgtttgc attcgacgca gggcaggaac tcagtgagca
     9001 cacggcgcca tttgacgcca tggtgctggt gctcgaaggg acactcgcat tgacaattgg
     9061 cggagcagtc gttcgcgcga cgccgggaac aattgttcgc atgccgtctc acatcccgca
     9121 tgccg
//
LOCUS       DBNGSH010000331         3315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2743342, whole genome shotgun sequence.
ACCESSION   DBNGSH010000331 DBNGSH010000000
VERSION     DBNGSH010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2743342"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="AC16CY_07790"
     CDS             complement(<1..1444)
                     /locus_tag="AC16CY_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR3353725.1"
                     /translation="MTSQWKGHGPEHPMRVDGVAESGASPAPGGVLTYEPFYGLTRKP
                     FSLSTDPRGLYKGPPHAAVLEDLLGAIRRREGLIVLTGDMGTGKTMLCRAAIYQLDRK
                     TFTTFVPDPYLTREDLLRMLLVGFGEVSVDDLSQGRLKGAPRADLACRLYEFLTSLES
                     VDAFATLVIDEAQHLTPALANEIRALSEMEAGRRLLQIILVGQRPLRGRLALPEMRHI
                     AQRVTTACDLPPLTREGVSGYVAYRLAAAGGVRDQVAFSSTALSLVYEASEGVPRLVN
                     RICDRALHYGCLDRTSQIGHAQVRRALQELELMVAAVDVAPPARVRSGEESQEPPRAS
                     GTPGEPAPRGLFQKTAAQPSPVADDLRALLDLPPVARQAGETMAAPYEQPLPRRRRSS
                     LGAKWVGALKTLALPAVALAVVLAAGAMTVSTVAARSGRIELPPPPASPLTLPPPVRP
                     RGRPATSTSTAPVLAAPAARPAPAGATQQTW"
     gene            1638..>3315
                     /locus_tag="AC16CY_07795"
     CDS             1638..>3315
                     /locus_tag="AC16CY_07795"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: protein motif:HMM:NF025179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HIR3353726.1"
                     /translation="MLANDALTPLRHDAHPTVPIKPAEVHLLDRVHALYRYRNVAIVA
                     FAVILTGAFLKAYWQTPLYRASVRLLIEIEDERTLALETLGTNRSYDYQDPEPYFQTQ
                     YRILTGRDLARRVVDKLDLTHAVEFGGSLAPAAPEAVVDAVASRVSVAPVRASRLVDV
                     SFVSADRNLASQAVNTLAEEYVTQNLDLRRQNMARSLEWLAQELVRQKNLVETSERAM
                     AQYREDQNALSLEDRQNIVIARLNLLNESATRARANRAQKESLFKRLESLRSDSSPDT
                     IPALLQNTYIQTMKGQVADLERRKALLSERYGDKHPEILTINASIDDANRQLRLELAK
                     AVDAIRHDYESALLEERTLLSALEEQKDIATDLDRKSVAYTVLQREAESNRDLYQALL
                     RREKELQVLANSQGNNVRLVERAGVPGAPFSPNIRRSAMLGGMVGLLVALGLAFGLDH
                     FNDTIRTPDDVTRRLGLPFLGLIPAVKGQPGRPVLSASSDFGEAIRALRTSLALSHTT
                     PGSAIVLVTSAQPLEGKTTTACNLAAALAYGGARVLLIDADMRRPGVHSGL"
BASE COUNT          564 a         1154 c         1088 g          509 t
ORIGIN      
        1 cccaggtctg ttgtgtcgcg ccagccggag ccggccgtgc ggcgggagcc gccagtacgg
       61 gcgccgtcga cgttgacgtc gcgggccgcc cgcgtggccg cacgggcggc ggcagcgtca
      121 gcggtgacgc gggagggggc ggcagttcga tgcgaccgga tctcgccgcc actgttgaca
      181 cggtcattgc accggcggcc agcacgacag ccaacgccac ggccggcagc gcgagcgtct
      241 tcaacgcccc aacccacttc gcaccaagcg aagaccgccg ccggcgagga agcggttgtt
      301 cgtagggcgc cgccatcgtc tctccggctt gccgcgccac cggcggaaga tccagcagag
      361 ctctgaggtc gtcggcaacc ggtgaaggct gggcggccgt tttctgaaag agcccccgcg
      421 gggcgggctc accgggcgtg cccgacgcgc gaggcggttc ctggctctcc tcgccggagc
      481 ggacgcgtgc cggcggcgcc acatcgacgg ccgccaccat caattcgagt tcctggagcg
      541 cccgcctcac ttgcgcgtgg ccgatctgag acgtccggtc gagacagccg tagtggagag
      601 cgcgatcgca gatacgattc accagcctcg gtacaccctc ggacgcctcg tagacgagac
      661 tgagagcggt cgacgagaac gccacctggt cgcgaacgcc gccggcggcg gcgaggcggt
      721 acgccacgta gccgctgaca ccttctcgcg tcagcggggg tagatcgcag gccgtcgtga
      781 cccgctgcgc gatatgccgc atctcgggaa gcgcgaggcg tccccggagc ggcctctgcc
      841 cgacgaggat aatctgcagg agccgccggc ccgcttccat ctccgagagc gcgcggatct
      901 cgttggcgag ggccggcgtc agatgttgtg cctcgtcgat caccagcgtc gcgaacgcat
      961 cgaccgactc gagcgatgtg aggaactcgt acaaccggca cgccagatcc gcccgcggcg
     1021 cccccttcag acgtccctgt gagagatcgt cgaccgacac ctctccaaag ccgaccagca
     1081 gcattcgaag caggtcttcc cgagtcaggt acggatcggg gacgaacgtg gtgaacgtct
     1141 tgcgatcgag ctgatagatc gccgcccggc acaacatggt ctttcccgtg cccatgtcgc
     1201 cagtcaggac aatcagcccc tcacggcggc ggatggcacc cagcaggtcc tcgagcaccg
     1261 cggcgtgggg cgggcccttg tagaggcctc gcgggtccgt ggaaagactg aacggtttcc
     1321 tcgtcagccc gtagaacggc tcgtaggtca gcacgccgcc cggggcaggc gaggctcctg
     1381 attcggccac gccgtcaacc ctcatcggat gttccggacc gtgacctttc cactgactcg
     1441 tcatcgaggc gtttccggtc ctccagcact gccatttatc ggctcggctg aggagccgtg
     1501 acaggccgac gccacgcggc gtgggcattc ccgtggccga tcctgcagtc gtgcgcaatt
     1561 gtgagttcta ctcacgagct tcggcggggc cgatacacga tcatcgggag tcgcccgtct
     1621 gcgttcccgt gtgcgcgatg ctcgccaacg acgccctgac gccgctgcgc cacgacgccc
     1681 atccgacggt gccaatcaag ccggccgaag tgcacctgct cgaccgggtg catgcgctct
     1741 atcgctaccg caacgtggca attgtcgcgt tcgcggtcat cctgacggga gcgttcctga
     1801 aagcctactg gcagacgccg ctctaccgcg cgagcgtgag gcttctcatc gaaatcgaag
     1861 acgagcgcac gctggcgctc gagacgctcg gcaccaatcg ctcgtacgac taccaggacc
     1921 cggagccgta cttccagacg cagtaccgca ttctcaccgg gcgcgacctg gctcgacgcg
     1981 tcgtcgacaa gctcgatctg acccacgccg tggagttcgg cggctccctc gcgccggccg
     2041 cgcccgaggc cgtcgtcgac gctgtcgcct cgcgcgtctc ggtcgctccg gtgcgggcca
     2101 gccgattggt cgacgtctcg ttcgtctccg ccgatcgcaa cctcgcgtcg caagccgtga
     2161 atacgctcgc cgaagagtac gtgactcaga acctggacct tcgccggcag aacatggccc
     2221 gcagcctcga gtggctcgct caggagctgg tgcgtcaaaa gaacctggtg gagacgagcg
     2281 aaagggccat ggcccagtac cgcgaggatc agaacgcgct gtcgctcgag gaccggcaga
     2341 acatcgtgat cgctcggctc aatctgctca acgagtcggc cacgcgcgca cgagccaacc
     2401 gtgcgcagaa ggagtcgctg ttcaagcgac tggagtcgct gcggtcggac tcgtcgcccg
     2461 ataccattcc ggcgctgctg cagaacacct acatccagac gatgaagggg caggtcgccg
     2521 acctggagcg ccggaaggcg ttgttgtcgg agcgctacgg ggacaagcac cccgagatcc
     2581 tgacgatcaa cgcctccatc gacgatgcga atcgtcagct tcggctggag ctggccaagg
     2641 ccgtggacgc gatccgtcac gactacgaat cggcgctgct cgaagagcgc actctcctca
     2701 gcgccctcga ggagcagaag gacatcgcca cggacctcga tcgcaagagc gtcgcataca
     2761 ccgtgctcca gcgcgaggcc gagagcaacc gcgacctgta tcaggcgctg ctgcgccgcg
     2821 agaaggagct ccaggtgctc gccaacagcc agggcaacaa cgtgcggctg gtcgagcgcg
     2881 cgggcgtgcc gggcgcgccg ttcagcccca acatccggcg cagcgccatg ctcggcggca
     2941 tggtcggcct cctcgtcgcg ctcgggctgg cattcgggct cgatcacttc aacgacacca
     3001 tccgcacgcc ggacgacgtg actcgaaggc tcgggctgcc gttcctcggc ctgattcccg
     3061 ctgtgaaggg acaacccggc cggccggtgc tgtccgcctc gagcgacttc ggcgaggcga
     3121 tccgggcgct caggacgtcg ttggccctca gtcacacgac tcctggcagc gcgatcgtgc
     3181 tggtgacgag cgcccagccg ctcgaaggca agacgacgac ggcatgcaac ctggcggctg
     3241 cgctcgcata cggcggcgca cgcgtcctgc tcatcgacgc cgatatgcgg cgtcccggcg
     3301 tgcactccgg gctcg
//
LOCUS       DBNGSH010000332         4795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3592627, whole genome shotgun sequence.
ACCESSION   DBNGSH010000332 DBNGSH010000000
VERSION     DBNGSH010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3592627"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="AC16CY_07800"
     CDS             complement(<1..1629)
                     /locus_tag="AC16CY_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3353727.1"
                     /translation="MASSRHPADSHDLIRVQGARVNNLKDISVEIPKRRLTVFTGVSG
                     SGKSSLVFGTIAAESQRLINETYSAFVQGFMPAMARPDVDVLEGLTTAIIVDQERMGA
                     NARSTVGTATDANAMLRVLWSRLGRPQIGPATAFSFNVPTRKASGAMTADQGTGKRTV
                     VRNVVYLGGMCPKCEGMGQVNDIDLKQLYDDTKSLKEGALTIPGYKAEGWYMRTFEAI
                     GIDTDKPIRKYSKKELHLLLYQEPTKVLVSKINLTYEGLIPRIRKSFLSKDVDAMNTS
                     IRGFVERAVTFTTCPDCGGTRLNEAALSSKIRKINIADACAMQINDLAAWVRALKEPS
                     VAPLLAKLRQTLDSFVEIGLGYLSLERPSGTLSGGEAQRTKMIGHLGSSLTDVTYVFD
                     EPTIGLHPHDIQRMNELLLRLRDKGNTVLVVEHKPESIAIADHVVDLGPSAGAAGGEL
                     VFEGTVDGLRASATLTGRHLSDRASLKPSVRKPTGAMKVRGARTHNLQNVSVDIPLGV
                     LVVVTGVAGSGKSSLIHGSVSGRDGVVAVDQTPIR"
     gene            1721..2827
                     /locus_tag="AC16CY_07805"
     CDS             1721..2827
                     /locus_tag="AC16CY_07805"
                     /inference="COORDINATES: protein motif:HMM:NF040043.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HIR3353728.1"
                     /translation="MSTVTWRRRGWLAAASGLALLMVAFSPRADGQARGQAPAGRGRA
                     VFSLSDPAPQDPALKGAIDLHAHQDPDSSGPSYTQAARSVDAIDLYTRAKAAGMRGFV
                     IKQHLDQTAGLAYYIRRLHPDLEVFGGMGSNLTTGTKVNPWAITHMAEIKGGWGRIVW
                     MPSWDSENSSHRITTRKPPSFVAVATCGAQPFWSNYPKPCPKGELLPEVKQALQVIAT
                     TKTRDSNGDLVLATGHNSYEEIVLMVREAVRVGVKRIILTHPLLDIVDMHDEQIKEVV
                     GMGPGIYAEFTALFGNPNGRPETVKRYVSGIRAAGVERAFVSSDSGQAGAAFHPDALA
                     NAARALRAQGFSERELDLLFKINPATILGLPTAN"
     gene            2840..3343
                     /locus_tag="AC16CY_07810"
     CDS             2840..3343
                     /locus_tag="AC16CY_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3353729.1"
                     /translation="MRNTSRSIAAIGGAVTLLLCVTASFGQGRGRGAPQNFVLQQAPT
                     DKAVPIPLETLKQYYADMDAKKLETLRMLEGGRFNVNIRRITNAESALVHPVTADVWV
                     VTEGSGTLTVNGTLEGGKIVGGDSRPLKTGDVVFVPAGVPHGVTGVTGSITWLNIRFD
                     TNYDAKP"
     gene            3355..3936
                     /locus_tag="AC16CY_07815"
     CDS             3355..3936
                     /locus_tag="AC16CY_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3353730.1"
                     /translation="MQRFIVGTLGVAVLLLQTAGFTQGKQPPDLATDITAAEVKAVLD
                     SPPGTGDRLITAVDMGSYNVGVGVLRRGATKPGAPVGAVNHDHITEVYYIISGSGTLV
                     TGGTVTNPRPMPATSEVVKVAVGPSNTATFDKPAQRRRVGPGDVVVIPPGVYHGFDEV
                     ATSIEYLAVRPDPNKVLPAGYVHPAAQAAKGAK"
     gene            3933..>4795
                     /locus_tag="AC16CY_07820"
     CDS             3933..>4795
                     /locus_tag="AC16CY_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3353731.1"
                     /translation="MKGLVLLILAAATASAQGPGRGQAPVQEWPSTMANPYRMVEKWP
                     AFGEIRSGAAIGIIPDGKGGVWLHHRSEPPIVHVDASGKIIKTFGDKMFVQAHGFCQD
                     RDGNFWAGDSGPFADNAASAGRGFQVFKFSPDGKVLLTLGKAGVSKAGEDTFIGPTAC
                     AIAPNGDVVIADGHWPRPTTAQQDGDRIVRYTRDGKFVAAFGRLGTGPGEFMGPHALA
                     FDSQGRLFVADRSNNRVQILDRDMKLVDEWRHFGRPSGVAILKDDTLVVSDSESNRQI
                     GGPPQAPEGGGT"
BASE COUNT          805 a         1581 c         1637 g          772 t
ORIGIN      
        1 gcggatcggg gtctggtcca ccgcgacgac accgtcccgg cccgacaccg agccgtggat
       61 cagcgagctc ttgcccgagc ctgccacgcc cgtcaccacc accagcacgc cgagagggat
      121 gtccacgctg acgttctgca ggttgtgcgt gcgggcgccg cgcactttca ttgcgcctgt
      181 cggctttcgg accgacggct tcagcgacgc ccggtcgctc aggtgacggc cggtgagcgt
      241 ggcgctggcg cgcagcccgt ctaccgtgcc ctcgaacacc agctcgccgc ccgcggcgcc
      301 ggcgctggga ccgagatcga cgacgtggtc ggcaatcgcg atcgattccg gcttgtgctc
      361 gaccacgagg acggtgttgc ccttgtcgcg caaccgcagc agcagctcgt tcatccgctg
      421 gatgtcgtga gggtgcagcc cgatcgtcgg ctcgtcgaac acgtaggtca cgtcggtgag
      481 cgacgacccg aggtggccga tcatcttcgt gcgctgcgcc tcgccgccag acagcgtgcc
      541 ggacggccgt tcgaggctca ggtagcccag cccgatttcc acgaacgagt cgagcgtctg
      601 ccgcagcttc gccaggagcg gcgcgactga gggctccttc agcgcgcgca cccacgcggc
      661 aaggtcgttg atctgcatcg cacaggcgtc ggcgatgttg atcttcctga tcttcgaaga
      721 cagggcggcc tcgttgagcc gggtgccgcc gcagtcggga cacgtggtga acgtcaccgc
      781 gcgctcgacg aacccgcgga tgctcgtgtt catcgcgtcc acgtccttgg acaggaacga
      841 cttcctgatc cgcggaatca gcccttcgta ggtgaggttg atcttcgaga ccaggacctt
      901 ggtgggctcc tgatagagaa gcaggtggag ctccttcttc gagtacttgc ggatcggctt
      961 gtccgtatcg atgccgatgg cctcgaaggt gcgcatgtac cagccctcgg ccttgtagcc
     1021 ggggatggtg agcgcaccct ccttgagcga cttcgtgtcg tcgtagagct gcttcagatc
     1081 gatgtcgttg acctgcccca tgccctcgca cttcgggcac atgccgccca ggtacaccac
     1141 gttgcgcacc acggtgcgct tgcctgtgcc ctggtcggcc gtcatcgcgc cgctcgcctt
     1201 ccgcgtgggg acgttgaacg agaaggccgt ggccggtccg atctgcggcc tgccgaggcg
     1261 gctccacagc acccgcagca tcgcgttggc gtcggtcgcg gtgccgactg tggaacgggc
     1321 gttggcgccc atccgctcct ggtccacgat gatggcggtc gtcagcccct ccagcacgtc
     1381 cacatcgggc cgggccatcg cgggcatgaa tccctgcacg aacgcgctgt aggtctcgtt
     1441 gatcagccgc tgcgattccg cggcgatggt gccgaatacg agcgacgact tgcccgatcc
     1501 cgacacgccg gtgaacaccg tcagccggcg cttggggatc tcgacgctga tgtccttcag
     1561 gttgttgacg cgggcgccct gcacgcggat gaggtcgtgg ctgtctgcgg ggtgtctcga
     1621 ggaggccatg cgcgcgatca tgtcatgtca acacaagtga actgtggtct agaatccagg
     1681 gcgatcgttg agcaccaggc tcggtgcaag aggacgcccg atgagtaccg tcacatggag
     1741 acgtcgcggc tggctggcag cagcctcggg gttggcgctt ctgatggtcg cgttctcgcc
     1801 gcgcgcggac ggccaggcac gcgggcaggc accggcgggg cgcggtcgtg ccgtcttctc
     1861 gctctcggat ccggcgcccc aggacccggc cctcaagggc gcgatcgacc tgcacgccca
     1921 tcaggatccg gacagcagcg ggccgagcta cacgcaggcc gcgcgatcgg tcgatgcgat
     1981 cgacttgtac acacgggcca aggcggccgg catgcgcggc ttcgtgatca agcagcacct
     2041 cgatcagacg gcagggctgg cgtattacat ccggcggctg caccccgacc tcgaggtgtt
     2101 cggtggaatg ggatcgaacc tgacgacggg gacgaaggtc aatccctggg cgatcaccca
     2161 catggccgag atcaagggcg ggtggggccg catcgtctgg atgccgagct gggactcgga
     2221 gaacagcagc caccgcatca cgacgaggaa gccgccgtcg tttgtggccg tcgcgacctg
     2281 cggcgcccag ccgttctggt cgaactatcc gaagccctgt ccgaaaggcg agctgctgcc
     2341 ggaagtgaag caggcgctcc aggtgatcgc gaccacgaag acacgggaca gcaacggcga
     2401 cctcgtgctg gctacggggc acaattcgta cgaggaaatc gtgctgatgg tccgtgaagc
     2461 cgtgcgagtc ggcgtgaagc gcatcatcct cacgcacccg ctcctcgaca tcgtggacat
     2521 gcacgacgag cagatcaaag aggttgtggg catgggcccg ggcatctatg cggagttcac
     2581 ggcgctgttc ggcaacccga atggccggcc cgagacggtg aagcgctatg tgagcggtat
     2641 ccgcgcggcc ggtgtcgagc gcgccttcgt gtcgagcgac agcggccagg cgggggcggc
     2701 gtttcacccc gatgcgctcg cgaatgcggc acgggcgctc cgggcacagg ggttctccga
     2761 gcgcgagctc gatctgctgt tcaagatcaa cccggcgacc atcctcggcc tgccgacggc
     2821 gaactgaagg tcagaagcca tgcgtaacac ctcccggtcc atcgccgcca tcggcggcgc
     2881 agtcacactg cttctgtgcg tcacggcctc cttcggccag ggccgcggcc gcggcgcacc
     2941 gcagaacttc gtcctgcagc aggcgccgac cgacaaggct gtgccgatcc cgctcgagac
     3001 gttgaagcag tactacgccg acatggacgc gaagaagctc gaaacgctgc gcatgctcga
     3061 aggcggccgc ttcaacgtca acatccgccg gatcaccaac gccgagagcg cgctcgtgca
     3121 tccggtgacc gccgatgtgt gggtcgtgac cgagggctcg ggcacgctga cggtgaacgg
     3181 cacgctcgaa ggcggaaaga tcgtcggcgg cgattcgagg ccgctcaaga cgggcgacgt
     3241 ggtcttcgtt cccgcggggg tcccgcacgg cgtgaccggc gtcaccggca gcatcacgtg
     3301 gctgaacatc cgcttcgaca ccaactacga cgcgaagccg taaaggggtg gcatatgcag
     3361 cgtttcatcg tgggcactct cggcgttgcg gtcctgctcc tgcagactgc tggcttcact
     3421 cagggcaagc agccgcccga tctggccacc gacatcaccg ccgcggaggt gaaggcggtg
     3481 ctcgactcgc ctccaggcac cggcgatcgg ctcatcaccg ccgttgacat gggcagctac
     3541 aacgtcggcg tcggcgtgct gcggcgtggg gccacgaagc ccggcgcgcc ggttggcgcc
     3601 gtcaatcacg accacatcac cgaggtctac tacatcatct ccggctcggg cacgctcgtc
     3661 accggaggca cggtgacaaa cccgcggccg atgcccgcga cgagcgaggt cgtcaaggtc
     3721 gccgtcgggc caagcaacac cgcgacgttc gacaagcccg cgcagcggcg ccgggtgggg
     3781 cccggcgacg tggtcgtcat tccgcccggc gtctaccacg gtttcgacga ggtcgcgacg
     3841 agcatcgaat acctggcggt gcggcccgat ccgaacaagg ttctgccggc cggttatgtg
     3901 catcccgcag cgcaagcggc gaagggcgcg aagtgaaggg cctcgtcctt ctgatcctgg
     3961 cggcagcgac ggcgtcggcg cagggaccgg ggcgaggcca ggcgccggtc caggaatggc
     4021 cgagcacgat ggccaatccg taccgcatgg tcgagaagtg gccggccttc ggcgagattc
     4081 gatcgggcgc ggccatcggc atcatccctg acgggaaagg cggcgtctgg ctgcatcacc
     4141 gatcggagcc gccgatcgtt cacgtcgatg cctcgggaaa gatcatcaag acattcggcg
     4201 acaagatgtt cgtgcaggcg cacggcttct gccaggaccg cgacggcaac ttctgggcgg
     4261 gagacagcgg tcccttcgcc gacaacgccg ccagcgccgg ccgcggcttc caggtgttca
     4321 agttcagccc cgacggcaag gtactgctca cgctcggcaa ggccggcgtg tcgaaggctg
     4381 gggaggacac gttcataggg ccgaccgcct gcgcgatcgc gccgaacggc gacgtcgtga
     4441 tcgccgacgg acactggccg cggccgacaa cggcgcagca ggacggcgat cgcatcgtcc
     4501 gctacaccag ggacggcaaa ttcgtggcgg cgttcggcag gctcggcacc gggccgggcg
     4561 agttcatggg tccgcacgcg ctcgcgttcg actcgcaagg ccgcctgttt gtcgccgacc
     4621 gctcgaacaa tcgcgtgcag atcctcgaca gagacatgaa gttggtggac gaatggcggc
     4681 acttcggccg gccgagcggc gtggcgatcc tgaaggacga cacgctcgtc gtgtcggatt
     4741 cggagtcgaa ccgtcagatc ggcggtccgc cgcaggcgcc ggaaggcggc ggcac
//
LOCUS       DBNGSH010000333         2547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1385173, whole genome shotgun sequence.
ACCESSION   DBNGSH010000333 DBNGSH010000000
VERSION     DBNGSH010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1385173"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="AC16CY_07825"
     CDS             <1..352
                     /locus_tag="AC16CY_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3353732.1"
                     /translation="LIIAREIDQQYEWTAHEPAGLKQGLEQSVIDVVKYNRDVKGLSD
                     RDATLITFGRTLYREHKVSSELWQKMVGHFGRQHTVQLMMIMGDYFRVGFMMNAVDQH
                     LPADRKALLPPLSR"
     gene            complement(369..1232)
                     /locus_tag="AC16CY_07830"
     CDS             complement(369..1232)
                     /locus_tag="AC16CY_07830"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3353733.1"
                     /translation="MRWILPLALLTTLAVTVNAQQAGRHAVTGMVLSVDAARKSVVVS
                     HDAVKGLMPAMTMPFEVREAKELSGLVPGTRVSFTLVLGKESGHIEQVKVVRYESVEQ
                     DPLTARRLRLLRSISGGAPKALAVGQPVPDFTLTDQVRAQVSLSQFRGKVVALNFIYT
                     SCVLPQFCYRVANHFATVRNRFKDRIGRDLVLLTVTFDPARDTPEKLAEYARQWQADP
                     ATWHFMTGSARDVERVCHMFGVDFFADEGLMSHSVRTALIDRRGSLVANVEGNQYTAA
                     QLGDLLQTVLK"
     gene            complement(1229..>2547)
                     /locus_tag="AC16CY_07835"
     CDS             complement(1229..>2547)
                     /locus_tag="AC16CY_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3353734.1"
                     /translation="QLGGLNIVQTDYNNDGLLDILVLRGAWETAQRKSLLRNNGNGTF
                     TDVTTESGLGRVPTRTQAAAWTDIDNDGHLDLFVGNEDGPAELFLNKRDGTFEDIAAR
                     AGVARGGFNKGVAAADYDNDGWPDLYVSNLGGSNYLYRNNHDRTFTEFGTAAGVPGPG
                     LGFPTWFFDYDNDGYQDLFVGSYVTSVDEVARAHLRLPRNGNTLKLYRNLHDGSFQDV
                     TRQAGLDMALMVMGSNFGDIDNDGFLDMYLGTGNPSYGALVPSVLLRNKGGTAFVDVT
                     ASSGTGELHKGHGVAFADLDNDGDEEIVFEVGGATPGDAHALRLFENPGHGGDWITLK
                     LVGTRTNRAAIGARITVTVQSVAGTRSIHRVVGSGGSFGASPLQQHIGLGRAAQKVDV
                     EVWWPVSDTRQRFLDVGKNQALEVTELEREYKPLRRVPQPLRGGTP"
BASE COUNT          419 a          845 c          834 g          449 t
ORIGIN      
        1 gctgatcatc gcgagggaaa tcgaccagca gtacgagtgg acggcgcacg agcccgccgg
       61 cttgaagcag ggactcgagc agtcggtcat cgacgtcgtc aagtacaaca gagacgtcaa
      121 ggggctctcc gacagggacg ccacgctcat caccttcggt cgcacgctgt atcgcgagca
      181 caaggtgagc agcgagctct ggcagaagat ggttgggcac ttcgggcgtc agcacaccgt
      241 gcagctcatg atgatcatgg gcgactactt cagggtgggc ttcatgatga acgcggtcga
      301 tcagcacctc ccggcggatc ggaaggcgct gctgcccccg ttgagtcgat gaccggcgag
      361 ccggccgatc acttgaggac tgtctgcagc agatccccga gttgcgcggc ggtgtactga
      421 tttccctcga cgttcgccac gaggctcccc cgccggtcga tgagggccgt ccggaccgaa
      481 tggctcatca gcccttcgtc cgcgaagaag tccacgccga acatgtggca gacgcgttcg
      541 acgtcccggg cgctgccggt catgaagtgc cacgtcgcgg gatccgcctg ccactggcgc
      601 gcgtactccg ccagcttttc cggcgtgtct cgggcgggat cgaacgtgac agtcagcagg
      661 acgaggtcgc ggccgatccg atccttgaac cggtttcgca ccgtggcgaa atggttggcg
      721 acgcgatagc agaactgcgg cagcacgcag ctcgtgtaga tgaagttcag cgcaaccacc
      781 ttgccgcgga attgcgacag cgacacctgc gcgcgtacct gatcggtcag cgtgaagtcg
      841 ggcaccggct gtccaacggc cagagccttt ggagcgccgc cgctgatcga ccgcagcagg
      901 cgcagccggc gcgcggtcag cggatcctgc tcgacgcttt cgtagcggac caccttcacc
      961 tgctcgatgt ggcccgactc cttgccgagc accagcgtga acgacaccct cgttccggga
     1021 acgaggccgc tcagctcctt cgcctcgcgc acctcgaacg gcatcgtcat tgccggcatc
     1081 agacccttca cggcgtcgtg cgagacgacc acgcttttcc gcgcggcgtc aacggagagc
     1141 accatgccgg tcacggcgtg ccgcccggcc tgctgggcgt tcacggtgac ggcaagtgtc
     1201 gtcagcagcg cgagcggaag aatccatctc atggcgtgcc gcctctcaat ggctgcggta
     1261 cccgccgcag cggcttgtac tcgcgctcca gctcggtaac ctcgagagcc tgattcttgc
     1321 ccacgtcgag gaagcgctgc cgcgtgtcgc tcacgggcca ccagacctct acgtccacct
     1381 tctgcgcggc gcgccccagg ccgatgtgct gctgcagcgg agacgcgcca aacgatccgc
     1441 cgctcccaac aacacggtgg atcgaccgcg ttccggccac gctctgcacc gtcaccgtga
     1501 tgcgggcgcc gatggcggcg cggttggtcc tggtgccgac gagcttcagc gtgatccagt
     1561 cgccgccgtg gccggggttc tcgaagaggc gcagcgcgtg cgcgtcgcca ggagtagcgc
     1621 cgcccacctc gaacacgatc tcttcgtcgc cgtcgttgtc gaggtcggca aaggcgacgc
     1681 cgtggccctt gtgtagttcg ccggtgcccg acgaggcggt gacgtccacg aacgcggtgc
     1741 cgcccttgtt gcgcagcagc acgctcggca cgagcgcgcc gtacgacggg ttgccggttc
     1801 cgagatacat gtcgaggaac ccgtcgttgt cgatgtcgcc gaagttcgag cccatcacca
     1861 tcagcgccat gtcgaggccg gcctgccgcg tgacgtcctg gaagctcccg tcgtgcagat
     1921 tgcgatacag cttcagcgtg ttaccgttgc gcggaagccg caggtgcgcg cgcgccactt
     1981 cgtcaacgga ggtcacgtac gagccgacga agaggtcctg gtagccgtcg ttgtcgtagt
     2041 cgaagaacca ggtcggaaac cccagtccgg gccccggcac gccggccgcc gtcccgaatt
     2101 cggtgaaggt ccggtcgtgg ttgttgcgat acaggtagtt cgagccgccg aggttcgaca
     2161 cgtagagatc gggccagccg tcgttgtcgt agtcggcggc agccacgccc ttgttgaatc
     2221 cgccgcgggc gacgccggcc ctggccgcga tgtcctcgaa ggtgccgtcg cgcttgttca
     2281 ggaacaactc ggccgggccg tcctcgttgc ctacaaagag atcgaggtgg ccgtcgttgt
     2341 cgatgtcggt ccaggcggcc gcctgcgtgc gtgtgggtac tcttcccagg ccgctctcgg
     2401 tcgtgacgtc ggtgaacgtg ccgttgccgt tgttgcgcag cagcgacttg cgctgcgccg
     2461 tttcccacgc tccccgcagc accaggatgt ccagaaggcc gtcgttgttg taatcggtct
     2521 gaacgatgtt caggccgccg agctgac
//
LOCUS       DBNGSH010000334         3101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3932179, whole genome shotgun sequence.
ACCESSION   DBNGSH010000334 DBNGSH010000000
VERSION     DBNGSH010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3932179"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="AC16CY_07840"
     CDS             complement(<1..247)
                     /locus_tag="AC16CY_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353735.1"
                     /translation="MRTNRPFRLLTSALLAAALVTLIGPAAFAQSSGQGEKLDSVLRH
                     RARQLSGRSRVIVEFNGDPDVRVFGSRSTRGRRLANRL"
     gene            complement(518..1861)
                     /locus_tag="AC16CY_07845"
     CDS             complement(518..1861)
                     /locus_tag="AC16CY_07845"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3353736.1"
                     /translation="MSVAVNRTAFAKHEASGDVEARFRALVQSPLRAGLLRFLAARPE
                     ESFDVESLMATFGRMRLDIDNCLRELVEFGIVGRGAGSPATYSAVRPENEPLLRLLDN
                     FLERRADISLEDQAPSVQRFREMIGRDEKMLVVFEWIRTAAKSDISVLILGPTGSGKE
                     VVARMIHELSRRGTENFQAVNCAALPDTLFESEIFGYEKGAFTGAHDRKPGRVELADR
                     GTLFLDEIGDLSLVAQAKLLRVLEDRRFERLGGQRAVQVDFRLISATNRPLDQFVRDS
                     RFREDLYYRVNAFSIRLPSLRERPADIAVLASRFIARYCAGNGLPLDAKAFSPEALAR
                     LHAYPWPGNIRELESTVSRAALSAPGRVIRDSDIEFLHPHVTTPSDAPTRLPTLAEAE
                     RAHIVRVLEATSWNKKEAARLLEISRGTLYRKIVEYELEPDARLSRKRPAVEPDR"
     gene            1983..>3101
                     /locus_tag="AC16CY_07850"
     CDS             1983..>3101
                     /locus_tag="AC16CY_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-hypusine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HIR3353737.1"
                     /translation="MPYRAPGAPDTSRPKYLTGKAIRYYRPRGSADLHHLIEEGFQAF
                     NAARLGEACRIFTDKMLLPEHDTTIALTVAGALTPAGLGGCMVELMERGLVDFMISTG
                     ANLYHDLHYALNFTLHRGSPFVNDVELYEEGIIRIYDVLFPATVLLETDAYVRDFLVR
                     WGVDGPVSTAELHYALGCDLLERKPGCQQYSVLAAAAAAGVPIYTSSPGDSSIGMNVA
                     YHELMNDSRLMIDPNKDVNEVCAFILAGKKNGCVILGGGSPKNFYLQGQPTLWEVYGI
                     PKGGNDYFIQITTDQVVWGGLSGATPAEAVSWGKVNPGVLPDTAVAYVDSTIGFPLFC
                     EYVVGSPRGRRPRKELVHKRDELVATLKKEAKLKTQNSK"
BASE COUNT          566 a          960 c         1030 g          545 t
ORIGIN      
        1 ggaggcggtt ggccagccgg cgcccccggg tcgagcggga gccgaagacg cggacgtcgg
       61 ggtctccgtt gaactccacg atcacgcgcg aacgccctga cagctggcgg gcgcggtggc
      121 ggagaaccga atcgagtttt tcgccttgcc cggacgactg ggcgaacgct gctgggccaa
      181 tcagcgtgac cagcgctgca gccagcagcg cggaggtcag tagacgaaac ggacgattcg
      241 tacgcattgc cggacagaga acagggcaag agtcctgccg aggtagctcg gtcgatctgg
      301 tctcagtgcc aaagtctttg aaataaaagt gttacgggtg gatccgtgtc tggagtcgcc
      361 tgaggtaatt gctcgcgggg ctcgaattgt gtgcagtacc tgcacacggt tttcgaggtc
      421 ggatcggcgt aaattgctgt tgagattgga gtaaacccac ggcagagcta tgttccaaat
      481 tggcaccatt ggtcgtgatc ccagaaccct tgtgttttca acggtctggc tcgaccgctg
      541 gtctttttcg agacaggcgg gcgtccggct cgagctcgta ctcgacgatc tttcggtaca
      601 aagtgccgcg gctgatctcg aggaggcggg ctgcttcctt cttgttccac gatgtggcct
      661 ccagcacgcg gacgatgtgc gcgcgctcgg cctcggccag cgtcggcagt cgcgtcggcg
      721 cgtcggaagg cgtcgtgacg tgcgggtgca ggaactcgat gtccgagtcg cggatcaccc
      781 gcccgggcgc tgacagcgcc gcgcgagaca ccgtgctctc gagctcgcgg atattgcccg
      841 gccaggggta cgcgtggagc cgggcgagcg cttccggaga aaacgccttc gcgtcgagcg
      901 ggaggccgtt ccccgcgcaa tagcgggcga tgaagcgact ggccagaacg gcgatgtcgg
      961 ctggccgctc acgcagtgac ggaagcctga tcgaaaaggc gttcacgcgg taatacaggt
     1021 cttcccggaa gcggctgtcg cggacgaact ggtcgagcgg ccggttggtc gccgagatga
     1081 ggcggaagtc cacctgcacg gcccgctgac cgccgaggcg ctcgaagcgg cgatcctcga
     1141 gcacccgcag gagcttggcc tgtgcgacca gtgacagatc accgatttcg tccaggaaga
     1201 gcgttcctcg atcggccagt tcgacgcgcc ccggtttgcg atcgtgcgca ccggtgaacg
     1261 ctcccttttc gtatccgaag atctccgact cgaacagcgt gtcgggaagc gccgcgcagt
     1321 tgactgcctg gaagttctcg gttccgcgac ggctgagctc atggatcatc cgggccacga
     1381 cttctttccc tgacccggtc ggccccagga tgagcaccga gatgtcggac ttggccgcgg
     1441 ttcgaatcca ctcgaacacg accagcatct tctcgtcgcg gccgatcatt tcccggaatc
     1501 gctggacgga tggagcctga tcctcgaggc tgatgtcggc gcggcgctcg aggaagttgt
     1561 cgagcaggcg caggagcggc tcgttttcgg gacgcacggc cgagtaagtc gcggggctgc
     1621 ccgcgccgcg gccgacaatg ccgaattcca ccagttcgcg gagacaattg tcgatgtcga
     1681 gccgcatgcg cccgaaggtc gccatgagcg actcgacgtc gaaggactct tccgggcgag
     1741 cggccaggaa gcgcaggagt cccgctctca gcggggactg gacgagtgcc cggaaacgcg
     1801 cttcgacgtc cccagacgct tcgtgtttag caaacgcggt gcgattgaca gctacactca
     1861 tcggattcaa cccgtgtatc ggcggatggc ggtgggcccg acagttacgc tcaattcggc
     1921 tgcgaattgc acgcggcccg atgcacggag tgttgcgggg gagaatgcta agctagccgt
     1981 cgatgccgta ccgcgcgcca ggtgcccccg acacctcccg gccgaagtac ctcaccggca
     2041 aggccattcg ctactaccgt ccccgcggca gcgccgacct gcaccacctg atcgaggagg
     2101 gtttccaggc gttcaatgcg gcgcggctcg gcgaggcgtg ccggatcttc accgacaaga
     2161 tgctgctgcc cgagcacgac acgaccatcg cgctcaccgt ggcgggggcc ctcacgccgg
     2221 caggccttgg cggctgcatg gtcgagctga tggaacgagg tctggtcgac ttcatgatca
     2281 gcacaggcgc gaacctctac cacgaccttc actacgccct gaatttcacg ctgcaccggg
     2341 gatcgccctt cgtcaacgac gtggagctgt acgaagaagg catcatccgc atctacgacg
     2401 tgctcttccc ggctacggtg ctgctcgaga ccgacgccta cgtccgcgac ttcctggtgc
     2461 gatggggcgt ggacggcccc gtctcgacgg ccgagctgca ctacgcgctc gggtgcgacc
     2521 tgctcgagcg caagcccgga tgccagcagt actcggtcct tgcggcagcg gcggcggccg
     2581 gagttccgat ctacacctcg tcgcccggcg acagctcgat cggcatgaac gtggcgtatc
     2641 acgagctgat gaacgacagc cggttgatga tcgatccgaa caaggacgtc aacgaggtct
     2701 gtgcgttcat cctcgcgggg aagaagaacg gctgcgtcat cctcggcggc ggttcgccga
     2761 agaatttcta tctgcagggg cagccgacgc tgtgggaggt gtacggaatc ccgaagggcg
     2821 gcaacgacta cttcatccag atcacgaccg atcaggtcgt ctggggcgga ctgtctggcg
     2881 cgacgcccgc cgaggccgtg agctggggca aggtcaaccc gggcgtcttg cccgatacgg
     2941 cagtcgccta cgtcgattcg accatcggct ttcccctgtt ctgcgagtac gtcgtcgggt
     3001 cgccgcgcgg gcgccggccg cggaaggaac tcgtgcacaa gcgtgacgag ctggtggcca
     3061 cactgaagaa agaggcgaaa ctcaaaactc aaaactcaaa a
//
LOCUS       DBNGSH010000335         1991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1046030, whole genome shotgun sequence.
ACCESSION   DBNGSH010000335 DBNGSH010000000
VERSION     DBNGSH010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1046030"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1991)
                     /locus_tag="AC16CY_07855"
     CDS             complement(<1..>1991)
                     /locus_tag="AC16CY_07855"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3353738.1"
                     /translation="AAIVLADSGDPSDEAAAEAALARIIDDTREIAAADRAEAATALA
                     HIENPRFRPLLVPLLHDRDTWVVRTAIRSARSMGAADGLFVPGLLSLLGQRVHKAEAR
                     ETLIGFGESIVPVLTHVLRDEHEHLWIRRHVPSTLAALGGPRAMTALVDSLNESDGFL
                     RYKAIRAIEKLRRDDPSLACPRQVLESAVVRETSRYYNGLTLQQNLLRHALDGGDSLL
                     AQALADKLRRAVDRIYCLLGLLYHVDDVAAARYTIEQGQTRRRAAAVEYLDNLLGGVV
                     RRRVMPILDDTPLAEKVRHANLVLKSRPRDLEDTLAQLVHDDDPVIAASAIHFVGRRG
                     LWSLADDLEYVLKHRAAGDRSVVETAAWALATRRGEVSDQSLPVVEIVDHIRLTPLFS
                     SLSIDELFRIAESGEEVRYPAADIAYRTGEPAEDVFFLIDGTLELIDDSRAARAVAAP
                     AVVNFEEVLQGTQLRSTVRSMNRTIGFRIASTAFLTMVSDNVLMAQRLFQLLLTEARL
                     GASAGLPSPATADPRLASAAADIASPFRQEPLLAGATATQLLALAAVATELPLVAGTG
                     LFEADAPPAIYQVLQGQVRIESPGQAPMLLSAGATFGLADTLAGTPSAWRALAATSGQ
                     ALRIDRERLFEVLADHVDLMQSVLTSILALRDREASPGQG"
BASE COUNT          345 a          643 c          707 g          296 t
ORIGIN      
        1 ccctggccag gcgacgcctc cctgtctcga agggccagga tcgacgtaag cacgctctgc
       61 atcagatcaa cgtggtcggc caggacttcg aacagccgct cgcgatcgat gcgaagagcc
      121 tggccactgg tggccgccag cgcgcgccac gcggaaggag tgcccgccag cgtatcagcc
      181 agaccgaagg tcgcgccggc cgaaaggagc atcggagcct ggccgggcga ctcgatgcgc
      241 acctgcccct ggagaacctg gtagatcgcc gggggcgcgt cggcttcgaa caagcccgtc
      301 cccgcgacca acgggagctc cgtcgccacg gcggcgagcg caagcagttg ggtcgccgtc
      361 gcgccggcaa gcagcggctc ctgccggaaa gggctggcga tgtccgccgc agctgaagcc
      421 aggcgcgggt cggccgtcgc aggagacggg agtccagcgc ttgcgccgag ccgcgcctcc
      481 gtgagcagca gctggaacag tctctgcgcc atcaacacgt tgtccgacac catggtcagg
      541 aacgcggtcg acgcaatacg gaagccgatg gtgcgattca tcgagcggac cgtcgaccgg
      601 agctgcgtcc cttggagaac ctcctcgaag ttgacgaccg ccggcgcggc gacggcgcga
      661 gcggcccggg aatcgtctat caactcgaga gtgccgtcga tgaggaagaa cacgtcttcg
      721 gcaggctcac ccgtgcggta cgcgatgtcg gcggccggat accgaacttc ctctccgctc
      781 tcagcaatcc ggaacagctc gtcgatcgac agtgaggaaa agagcggtgt gagccgaatg
      841 tggtcgacga tttccacgac tggaagcgac tgatccgaca cctcgccccg gcgcgtggcg
      901 agcgcccagg ccgcggtttc taccacgctc cggtcgccgg cggcccggtg cttgagcacg
      961 tattccagat catcggcgag cgaccataaa ccacgccggc caacgaagtg gatggccgag
     1021 gccgcgatca ccgggtcgtc gtcgtgaacg agctgggcca gcgtgtcttc gaggtctcgc
     1081 ggcctgctct tcagcacgag gttggcgtgg cgcactttct cggccagtgg cgtgtcgtcg
     1141 aggatcggca tcacgcggcg tcgaacgacg ccgccgagca ggttgtcgag gtactccacg
     1201 gcggcggcgc ggcgccgggt ttgaccctgc tcgatcgtgt agcgcgcggc cgcgacgtcg
     1261 tcgacgtgat acagcaggcc gaggaggcag tagattcggt cgacggcccg acggagcttg
     1321 tcggcaagcg cctgggccag gagagagtcg cctccgtcga gcgcgtgtcg cagaaggttc
     1381 tgctgcagcg tgaggccgtt gtagtagcgg gacgtctcgc gcaccacggc cgactcgaga
     1441 acctggcgag gacaggcgag agatgggtca tcgcgccgca gcttctcgat cgccctgatg
     1501 gccttgtagc gcaggaagcc gtcagactcg ttcagcgaat cgacgagggc ggtcatcgcg
     1561 cgaggaccgc cgagcgcggc caacgtggat ggcacgtggc ggcggatcca gagatgctcg
     1621 tgctcgtccc tgagcacgtg ggtcagaacc gggacgatgg attcgccgaa cccgatgagc
     1681 gtttctcgag cttcagcctt gtgcacgcgc tggccgagca acgacaggag ccccggcacg
     1741 aacagcccat cggcggcccc catggatcgc gcgcttcgga tcgccgttcg aacgacccag
     1801 gtgtcccggt cgtgcaggag ggggacgagg agcggacgga accgggggtt ctcgatatgc
     1861 gcgagcgctg tggcggcctc ggcgcgatcg gccgcggcga tctcccgcgt gtcgtcgatg
     1921 atgcgggcga gcgcggcttc ggcggcagcc tcatctgacg gatcgcccga gtcggccagg
     1981 acgatcgctg c
//
LOCUS       DBNGSH010000336        13253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_366531, whole genome shotgun sequence.
ACCESSION   DBNGSH010000336 DBNGSH010000000
VERSION     DBNGSH010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 13253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13253
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_366531"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1117)
                     /gene="corA"
                     /locus_tag="AC16CY_07860"
     CDS             complement(113..1117)
                     /gene="corA"
                     /locus_tag="AC16CY_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR3353739.1"
                     /translation="MPEVLDQARAAPVIDCAAYAGGRRVADVGIDQIRDTLERPDQFV
                     WLGLYEPEKDILRGVQQQFGLHDLAVEDAYNAHQRPKLELYEDSLFVVLRTAHLATAP
                     RHLEFGETHVFLGRNYVVTVRHGSLRSHIGVRQRCESTPHLLAKGPGYVLYALMDFVV
                     DQYLPIVQQFEEEVDELEDVIFGETASGDATVRVYQLKRDLLALRRAIAPLVEVCNRL
                     MRFELPHIPEDTRVYFRDVYDHIVRLNETIDAQRELLTTALEAHLSIISHAQNEHMKR
                     ITAWAAMIAVPTMIAGIYGMNFRNMPELGWSYGYYASIGVMAAASAGLYVGFKRSGWL
                     "
     gene            complement(1160..1831)
                     /locus_tag="AC16CY_07865"
     CDS             complement(1160..1831)
                     /locus_tag="AC16CY_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3353740.1"
                     /translation="MEPARTSVSDGFRVKELHSELEAVRTSVVCALNQLLDLKDLNTG
                     VHSTRLAEWAVRIGRDLGLDDGYLRDVEMAAILHDIGKIGTPDAVLKKPSQLTDAERT
                     VIRKHPEYGWAVLRVVPGLERVSLFVLHHHESYDGSGYPAGLRGDEIPIGSRILAVMD
                     AFDAMISSRPYRRGLSLEEAVRRLEAGMGTQFDPIVTPCFVAMARSEAATVFDATGSS
                     LDAAL"
     gene            complement(1833..3158)
                     /locus_tag="AC16CY_07870"
     CDS             complement(1833..3158)
                     /locus_tag="AC16CY_07870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3353741.1"
                     /translation="MKRRSATLLSLFAVVLLGLTPVQAYAGERLIVRTSSLLDGLTVV
                     RSACRILGCQVLYGLDGTLGKLFLVELPPLLDADLAVSLIDALPGIAGAEIDLKGSTL
                     AAVGTTVPAALYDDEPVHYYGATVRGGYARQPAAEIVGIAETQSQYRLSGQGVTVAII
                     DTGVDPSHPALQAVLLPGYDFTRNRSGGSERSDVNQSTVAVLDGAQPGFVNQSTVAVL
                     DQSTVAVLDGPDYAAFGHGTMVAGIVHLVAPQARILPLKAFGADGSGYTSDVLRAIYA
                     AVRDGAKVINMSFSFSTQSRELERAVDYATDRGIVAVASTGNDGRRVDVYPASLSTVI
                     GVASSTDWDTLSDFSNYGPSVAWIAAPGEAVVSTYPFGTYAAAWGTSFSTPFAAGTAA
                     LLAEVSRHINESLAAEAEGQGVWISNEVRKGRLHAPTAVRAWRTRLGLP"
     gene            complement(3163..3951)
                     /locus_tag="AC16CY_07875"
     CDS             complement(3163..3951)
                     /locus_tag="AC16CY_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353742.1"
                     /translation="MSIFLAIALFFSQVAAPIGMSVLSMRTTPPGDAGGLRHVKDHFS
                     SPQWVDRVRGRLEPRVVAEMDEHLRVGCRDCLAAFDTWQGVAAFAEEERSSTPPADAI
                     RVVKSYLAQRNRLEDRPGPSTTPWAIATFATLVFDSLHASAAGVRAAAAFTRHLVFEA
                     KSLVVDLHVDETSPTGGVLLIGQVADSKDPGHVLENVWLSLVNERGEVSMFPTNEYGE
                     FNCTFTRGRNLKLMVYAASQVVAIPLDSLFDPAQDTPGSSERKR"
     gene            complement(3948..6833)
                     /locus_tag="AC16CY_07880"
     CDS             complement(3948..6833)
                     /locus_tag="AC16CY_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3353743.1"
                     /translation="MTVAAATPRVKPIAGSRVSRAIASLPDESARERFFARHARLINP
                     QLAQRLLESARDQLRVDGRRSLTFAQSAHSLGERLGDERLRGLGLRAMANALSIMGDN
                     QAAIELHVQAIAAFERVGDEPERARTLSASLQPLLLLGRYDEALEAADRARASFLAHG
                     DAVRLARLDITIGNLLHRQDRFAEAMVCYERAHQAVSSLEDVDGIVAVLHNKAVTLTS
                     LNDFRAALATYESGRRLAAARGLDQLVAQADYNIAWLYYLRGEYSRAIEILHTAAAAS
                     KRTGDAYHAALSLLDLSEIYLELNLSVDAREMAEQAHTRFNELGMGYEAAKALANAAT
                     SHGQEGKTFRALELFAQARAGMVREQNHVWPSLIDLYQAILLVGEGRLFEARRLCLTA
                     RGCFEQSRLAAKAAMCDLLLARIALRLDDPADGLRRCDEALTRLAGVESPILTYHARL
                     LQGHSLSRAGDAEAARASYQTARTALESLRSRLNGEELKIAFVKNKGEVYEQLVQLCL
                     DRGDGQSVEEAFSHIEHAKSRTLFDLMFQPVHALAREDGSESQLAHSIRELREELNWY
                     YHLVEREQLRPGDQSLERLATFQREIGTREKEMARALRELKLSDSARSELHAPSVCSL
                     NDIRSTLPRDAALVEYFQVGDRIILCVVDADHVEMRPVSLASRVAAHVRMLQFQFSKF
                     RVGSDYVRTFHDTLLRATQSHLAALFDELIAPLWERLQGRRLVVVPHGLLHYVPFHAL
                     YDGARYVTDSCTVSYAPSASIFATCQQQAAGGGQGVLVLGVPDEQAPLIETEARAVAQ
                     AAPDARLFIGEEATEQVLRSSGRESRIVHIASHGYFRPENPMFSGIRLGDTYLNVYDL
                     YRLRLRADLVTLSGCATGANVAAAGDELLGITRGLFCAGARTLLLSLWNVHDQSTERL
                     MAPFYRRVADGASPVIALRDTMLEVREEFPHPYQWAPFVVTGKSGQV"
     gene            complement(6830..7414)
                     /locus_tag="AC16CY_07885"
     CDS             complement(6830..7414)
                     /locus_tag="AC16CY_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3353744.1"
                     /translation="MNALANLPPFQQGTRSDRELVAACLSGEEDAWGALVDRYSRLIF
                     SIPLRQGLGREEAADIFQAVCLDLIVELPRLRDPQALPAWLIRTTARKAAKWRHRNQR
                     YVADEGGGTEAAASEEPPDALIEHCQRSQALRDGIASLPERCRVMVQMLFFETPARPY
                     REVAEALGVATGSIGFMRGQCLDRLRSVLRKAGL"
     gene            complement(7411..9189)
                     /locus_tag="AC16CY_07890"
     CDS             complement(7411..9189)
                     /locus_tag="AC16CY_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3353745.1"
                     /translation="MRKGPSMRLGVSCLLAALFATLPAGAAAQPLPIAAGEEVLVERD
                     GSGHYGRRLVVALRAEPRTFNPAIAADAPSREVIGRMTGDLIHINRSTQLTEAALARG
                     WTRSTDGREYTLTLRKGIRFSDGQPFDADDVVFTFRAMLDERTGAPQRDLLIVGGQPV
                     RVTKVDAHTVRVALAQPYAAAERLFDSIAILPEHLLAGAYARGALAEAWPLTTSAGAI
                     AGLGPYRLKEYTAGQRLVLERNPFYWKADAAKRRLPYMDEIVFLFAGTEDAQIIRFQS
                     GESDILNRASADNFTALARNGSARHRLEDLGPGLEYNFLVFNQNDLSGKGLPQIESRQ
                     RWFGDLGFRRAVSRAVDRESIARLVYRGRAVPLWGNVSPGNRRWVNASIARPARSVSE
                     ARAILKTSGFSWRPDGALLDQQGQRVAFTIVTSSSNTQRTEMATLVQADLKELGMDVQ
                     VVPLEFRALVDRVFQTFDYDASILGLGGGDADPNSEMSVWLSTGSNHLWRLGQKTPAT
                     AWEAEVDRLMAQQLVTLDSRERKRLFDRVQELVAEQLPFIFLAAPHILVGARPDLGNF
                     QPSVLDHYTLWNVDRLFIQPDGSQRR"
     gene            complement(9186..10214)
                     /locus_tag="AC16CY_07895"
     CDS             complement(9186..10214)
                     /locus_tag="AC16CY_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353746.1"
                     /translation="MTRGRARALVVVLAALHLLVLLAGFVAPYDPVDQARALPFAPPV
                     RLHVIDADGRFHPRPFVYALVPDPATADGYMEDRSRRFPVQMMVRGARYSVLPGLESN
                     LHLVGVETPGRLFLLGTDGFGRDLLSRLLHGSRVSVGAGLLATLSALLIGMVLGTVAG
                     FSGGRIDGGVMWAANLFMAVPWLYLLFAARAALPLHIDTRATFLLLVTVLGVVGWARP
                     ARLIRGVVLSARERTYVLAARGFGAKPLYLLRRHLLPHTFGVALTQAGLLVPQYILAE
                     VTLSFLGLGVGEPTPSWGTMLGSLQQYDVLTSYWWMFAPAVALVVTAVTYHAVTSAVH
                     GRIRLVTT"
     gene            complement(10211..11170)
                     /locus_tag="AC16CY_07900"
     CDS             complement(10211..11170)
                     /locus_tag="AC16CY_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353747.1"
                     /translation="MRVVFRYVARSLITLASVSVFSFLLLELAPGDFFTDLRAETRVG
                     PETVEALRNQYGLTRSPLARYWQWLQSFVSGDLGFSLAHRAPVASVLWVRAGNTLLLT
                     VPALLVTWGLALPLGIWAARRRGRWPDRLCGGATSTLLALPDLLVALLLLLLALRTGL
                     FPTGGMLSPGAEDMSVTARVRDFAAHAFLPLCALVVTTLPAVFRHVRASMIEAAGAPA
                     VQAARGLGIRPSRLTLRFVLPLAANPLVSLAGLSIATLLSASLLIEVVMSWPGLGPLL
                     VEALLAKDVHVVLSASMLATGLLVLGNLAADLTLVGIDPRIRA"
     gene            complement(11167..12741)
                     /locus_tag="AC16CY_07905"
     CDS             complement(11167..12741)
                     /locus_tag="AC16CY_07905"
                     /inference="COORDINATES: protein motif:HMM:NF007739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353748.1"
                     /translation="MPLLQITDLSVRYEDRPPAIDGVSMSLGPGEIVGLSGPSGCGKT
                     TLALALMGLLPPAAELRGSIQFQGRELTGLTDRDLQTVRGAELGIVFQESSLALNPLL
                     RAGVQIAEVVRAHERCDRGAALERARAAMAEVGLGENLPRIFDAYPHELSGGERQRVL
                     IAQAIVCRPRLVIADEPVASLDADVRADVLGLIRTLNEQLGISFLLITHSADVLATTA
                     TRVIKMTAGRIDDRPEVPTRTTTRVRRTSPRVEGGERGTGTGEPLVELRGLRRTYTHR
                     RMFSGRRHEVEALRGVDMDIERGSTLGLTGRSGCGKSTLARCMAGLEPADAGHIRING
                     TDIAALRGRALLPYRNQLQVIFQDSAAALNPRFTAGQIVSEPLVIQGIGTAGERRHRA
                     AELMALVELPSDRLESRPEEFSGGERQRLAIARALAVDPAVLIFDESFSGLDQATRSR
                     ILGVLMHLQAARGLTFVCISHDLALLAEFASEVALMREGRIVERGVPDEVLYRAYAGH
                     RGDTLSPDLAPIGATA"
     gene            12976..>13253
                     /locus_tag="AC16CY_07910"
     CDS             12976..>13253
                     /locus_tag="AC16CY_07910"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3353749.1"
                     /translation="MTRATRVVSCLAVLASWAAALNHPALAHHSFAMFDTAKRVTLAG
                     TVTAFEWTNPHAYIEIDVEESGRVRHWSIELGSTSILMQGGWKFNDLKR"
BASE COUNT         2275 a         4463 c         4512 g         2003 t
ORIGIN      
        1 gacccaccgt agcgaaacat cggcccgatc tccgcgccca gacactgttc atagaacttg
       61 agcgcggcct cgcagtcgcc cttgaagctc agaatcggaa ccatcctcat cgctagagcc
      121 atcctgaccg cttgaacccg acgtacagcc ccgcggaggc cgcggccatc acgccgatgg
      181 aggcgtaata gccgtagctc caacccagct cgggcatgtt ccggaagttc atgccgtaga
      241 tgccggcgat catcgtcggg acggcgatca tcgccgccca ggccgtgatg cgcttcatgt
      301 gctcgttctg cgcgtgcgag atgatcgaca ggtgcgcctc gagcgccgtc gtcagcagct
      361 cgcgctgcgc gtcgatcgtc tcgttcaacc gcacgatgtg gtcgtagacg tcgcggaagt
      421 acacccgcgt gtcctcgggg atgtggggca gctcgaaccg catcaggcgg ttgcagacct
      481 cgacgagcgg cgcgatggcg cgccggagcg cgagcaggtc gcgcttgagc tggtacacgc
      541 gcaccgtggc gtcgccgctg gccgtctcgc cgaagatgac gtcctcgagc tcgtccacct
      601 cttcctcgaa ctgctggacg atcggcaggt actgatcgac gacgaagtcc atgagcgcgt
      661 agaggacgta gcccggtccc ttggccagca ggtgcggcgt ggattcgcat cgttgccgga
      721 cgccgatgtg cgaccggagc gacccgtgcc tgaccgtcac gacgtagttg cgacccagga
      781 agacgtgcgt ctcgccgaac tccaggtgcc ggggcgccgt ggccaggtgc gcggtacgca
      841 gcacgacgaa cagcgagtct tcgtacagct cgagcttggg acgctggtga gcgttgtagg
      901 cgtcctcgac cgccagatcg tgcaggccga actgctgctg cacgccgcgc aggatgtcct
      961 tctccggctc gtacagcccc agccacacga attgatcggg gcgttccagc gtgtcccgga
     1021 tctggtcgat cccgacgtcg gcgacgcgcc tgccgccggc ataggcggcg cagtcgatca
     1081 ccggcgccgc gcgggcctgg tcgaggacct ccggcatgta gacagcttac cccgatgcga
     1141 cgcgactgca aggggttggt cagagcgccg cgtcgaggct cgagcccgtc gcgtcgaaga
     1201 ccgtggcggc ctcgctgcgg gccatggcga cgaagcaggg agtgacgatc ggatcgaact
     1261 gcgtgcccat gccggcttcc agccggcgga cggcttcctc gagcgacagg ccgcggcggt
     1321 acggcctcga cgagatcatg gcgtcgaagg catccatcac cgcgagtatc cgcgatccga
     1381 tcgggatctc gtcgccccgc aggcccgcgg gatagccgga gccgtcgtag gactcgtggt
     1441 ggtgcagcac gaagaggctg acacgctcga ggccagggac gacgcgcagc acggcccagc
     1501 cgtactccgg atgcttgcga atcaccgtac gctccgcgtc ggtcaactga gagggcttct
     1561 tcagaacggc gtccggtgtg ccgatcttcc cgatgtcgtg gaggatcgcg gccatctcga
     1621 cgtcccgcag gtacccgtcg tcgagtccga ggtcgcgtcc gatccgtacg gcccattcgg
     1681 ccagccgtgt cgagtgaacc cctgtgttga ggtccttcag atccagcaat tgattgagcg
     1741 cgcagacgac cgacgtgcgc acggcctcga gttcgctgtg cagctccttg acccggaagc
     1801 cgtcggacac cgaggtgcgc gcgggttcca cgctacggca gccccaggcg agtccgccac
     1861 gcccggacgg ccgtcggcgc gtggagtcgg cccttgcgga cctcgttgga gatccagacg
     1921 ccttgcccct cggcctcggc cgcgagcgac tcgttgatgt gccgggagac ttccgcgagt
     1981 aacgccgccg tgcccgcagc gaacggcgtg ctgaaggacg ttccccacgc ggcggcatac
     2041 gtgccaaacg gatacgtgct caccacggcc tcgcccggcg cggcgatcca ggccacgctc
     2101 ggtccgtagt tcgagaagtc ggacagtgtg tcccaatcgg tgctcgacgc cacgccgatc
     2161 accgtcgaga gcgacgccgg gtacacatcg acgcgccggc cgtcgttgcc ggtggaggcg
     2221 acggcgacga tgcctcgatc cgtggcgtag tccacggcgc gctccagctc gcgtgattgg
     2281 gtcgagaagc tgaagctcat gttgatgacc ttcgcgccgt cgcgcaccgc cgcgtagatg
     2341 gcgcgcagga cgtcggaggt gtaaccgctg ccgtccgctc caaacgcctt gagggggaga
     2401 atccgggcct gcggagcgac caggtgcacg atgccggcca ccatggtgcc gtggccgaac
     2461 gccgcgtaat cggggccgtc gagaaccgcc accgtggact gatcgaggac cgccacggtc
     2521 gactggttca cgaatccggg ctgcgcgccg tcgaggaccg ccaccgtgga ctggttgacg
     2581 tcgctgcgtt cggacccgcc gctgcggttg cgcgtgaagt cgtatcccgg cagcaggacc
     2641 gcctgcagcg ccgggtggct cgggtcgacg ccggtgtcga tgatggcgac ggtgacgccc
     2701 tgccccgaga ggcggtactg gctctgcgtc tcggcgatac ccacgatctc ggcggccggc
     2761 tgtcgggcgt acccgccccg cacggttgcg ccgtagtagt ggacgggttc atcgtcgtac
     2821 agcgctgcgg gcaccgtggt gccgacagca gccagggtcg atcccttcag gtcgatttca
     2881 gccccggcga tccccggcag cgcgtcgatc aggctgaccg cgaggtcggc gtccagcagc
     2941 ggcgggagct cgacgagaaa cagcttgccc agcgtcccat cgagcccgta cagcacctgg
     3001 catccgagga ttcggcacgc ggacctgacg acggtcaggc cgtcgagcag actcgacgtg
     3061 cggacgatca gccgttcccc ggcgtaggcc tgcaccggcg tcaggccgag cagcacgacg
     3121 gcgaacagcg aaaggagcgt ggcagagcgg cgcttcatgt cgtcaccttt tacgttccga
     3181 ggatccgggc gtgtcctgcg cggggtcgaa cagtgaatcg aggggaatgg cgaccacctg
     3241 ggaggcggcg tacaccatca gcttcagatt cctgccgcgg gtgaacgtgc agttgaactc
     3301 gccgtattcg ttcgtcggga acatgctcac ttccccccgc tcgttgacga gcgacagcca
     3361 gacgttctcg agcacgtgcc cgggatcctt cgagtcggca acctgcccga tcagcaagac
     3421 tccacccgtg ggcgaggtct cgtcgacgtg gagatccacg acgagtgact tcgcctcgaa
     3481 cacgagatgc ctcgtaaagg cggccgccgc gcgaacgccc gcggccgatg cgtgcaggct
     3541 gtcgaagacg agcgttgcga acgtcgcgat ggcccagggt gtcgtggacg gtcccggccg
     3601 atcctccagg cgattccgct gtgccaggta ggacttcacg acgcggatgg cgtcggcggg
     3661 cggcgtggac gaccgctcct cctcggcaaa ggccgccacg ccctgccacg tgtcgaacgc
     3721 ggccaggcag tcgcggcagc cgacacggag atgttcgtcc atctcggcca cgaccctcgg
     3781 ttcgagccga ccgcgaacgc ggtcgaccca ctgcggcgat gaaaagtgat ctttcacgtg
     3841 acgtagtccc ccagcgtccc ccgggggcgt cgtgcgcatc gagaggacgc tcataccgat
     3901 aggggccgcg acctggctga aaaatagagc gatcgcaaga aaaatactca tacctgcccg
     3961 gatttgccgg tgacgacaaa cggcgcccat tgataggggt ggggaaactc ctcccgaacc
     4021 tcgagcatgg tgtcgcgcag cgcgatcacg ggagacgctc cgtcggcgac acgtcgatag
     4081 aagggcgcca tgaggcgctc ggtgctctgg tcgtggacgt tccagaggct gagcagcagc
     4141 gtcctggcgc cagcgcagaa cagccctcgt gtgatgccga gcagctcgtc accggcggcg
     4201 gccacgttgg ctcccgtggc gcagccgctc aacgtgacca ggtccgcccg caacctgagc
     4261 cggtacaggt cgtagacgtt caggtaggtg tcgccgaggc gaattcccga gaacatcggg
     4321 ttctccggcc tgaagtaccc gtgcgacgcg atgtgcacga tccggctttc gcgccccgac
     4381 gaccgcagca cctgttcggt cgcctcttca ccaatgaaca ggcgcgcatc gggggctgcc
     4441 tgggccaccg cgcgcgcttc ggtctcgatg agaggcgcct gctcgtcagg cacgccgagc
     4501 acgagcacgc cctgcccgcc gccggcggct tgctgctggc acgtcgcgaa gatgctggcg
     4561 ctcggcgcgt aggagacggt gcacgagtcg gtcacgtacc gtgccccgtc gtagagggcg
     4621 tgaaacggca cgtagtgcag gaggccgtgg ggaacgacca cgagccgccg gccctggagc
     4681 cgctcccaca gcggggcgat caactcgtcg aacagcgcgg cgagatgtga ctgagtcgcg
     4741 cggaggagcg tgtcgtggaa cgtgcggacg taatcggagc cgacgcggaa cttcgagaac
     4801 tggaactgca gcatgcgcac gtgcgcggcg acgcgcgacg cgagcgagac cggcctcatc
     4861 tcgacgtggt cagcgtccac cacgcagagg atgatgcggt cgccaacctg gaagtactcg
     4921 acgagcgcgg catcccgcgg gagcgttgac cggatgtcgt tcagcgagca gaccgacggc
     4981 gcgtgcagct cggaccgcgc ggaatccgac agcttgagct cgcgcagggc gcgggccatc
     5041 tctttttccc tggtgccgat ttcccgctgg aacgtggcca gccgctcgag ggactggtca
     5101 cctggacgaa gctgctcgcg ttccaccagg tggtagtacc agttcaactc ctcgcggagc
     5161 tcacggatgg aatgcgcgag ctggctctcc gagccgtcct cgcgcgcgag ggcgtgcacc
     5221 ggctggaaca tcaagtcgaa cagcgtgcgc gacttcgcgt gctcgatatg cgaaaacgcc
     5281 tcctccaccg actggccgtc cccgcgatcg aggcagagct gcacgagctg ttcgtacact
     5341 tcgcccttgt tcttgacgaa ggcgatcttc agctcctcgc cattcagccg gctgcgcagg
     5401 gattcgaggg ctgtgcgcgc ggtctgatac gacgcgcggg cggcctcggc gtctcccgct
     5461 cgcgagagcg agtgtccctg aagcagccgc gcgtggtagg tgaggatcgg gctctccacg
     5521 ccagccagcc tggtcagagc ctcgtcgcag cggcgcagcc cgtccgccgg gtcgtccagc
     5581 cgcagcgcaa tgcgggccag caggagatcg cacatcgcgg ccttggcggc gagccgggac
     5641 tgctcgaagc atccacgcgc ggtcagacac aggcggcggg cctcgaagag tcgcccctcg
     5701 ccgaccagga ggatggcttg gtacagatcg atgagcgacg gccagacatg attctgctcg
     5761 cgcaccatcc cggcccgtgc ctgggcgaac agctcgaggg ctcgaaaggt cttcccctcc
     5821 tgaccgtgcg aggtggcggc gttggccagc gctttggcgg cctcgtaccc catcccgagc
     5881 tcgttgaacc tggtgtgcgc ctgttcggcc atctcgcgcg catccacgct caggttcagc
     5941 tcgaggtaga tctccgacag atcgaggagc gacagcgccg catgatacgc atccccggtc
     6001 cgcttggagg cggccgcggc ggtatgcagg atctcgatcg cccggctgta ctcaccgcgc
     6061 aggtagtaca gccacgcgat gttgtagtcc gcctgcgcca cgagttgatc cagcccgcgc
     6121 gcggccgcca ggcggcggcc gctttcgtag gtcgccagag cggcgcggaa atcgttgagc
     6181 gaggtcagcg tgaccgcctt gttgtgcagg accgcgacga tcccgtcgac gtcttcgagc
     6241 gatgacacgg cctgatgggc gcgctcgtag cagaccatcg cctccgcgaa ccgatcctgg
     6301 cgatgcagga ggttgccgat cgtgatgtcg agccgcgcca gtcgcaccgc gtcgccgtgc
     6361 gccaggaaag acgcccgtgc acgatccgcg gcttcgagcg cttcgtcata gcgtcccagc
     6421 aggagcagcg gctggagact cgcactcaag gtgcgcgcac gctcgggctc gtcgcccacg
     6481 cgctcgaatg cggcgatcgc ctggacgtgg agctcgatcg cggcctggtt gtcgcccatg
     6541 atcgagaggg cgttggccat cgcgcgaagg ccgagaccgc ggagccgctc gtcgccgagc
     6601 cgttccccca ggctgtgcgc gctctgggcg aaggtcagcg agcgacggcc gtccacccgg
     6661 agctggtcgc gcgcgctctc gagcaaccgc tgcgcgagct gcgggttgat cagccgcgcg
     6721 tgacgcgcga agaagcgctc gcgggcggac tcgtcaggca ggctcgcgat cgcgcgcgag
     6781 acccgggagc ccgcgatcgg cttgacgcgc ggcgtcgccg cggcgaccgt cacaggcccg
     6841 cctttcgaag gactgaccgc agccgatcga ggcactggcc gcgcatgaag ccgatcgaac
     6901 cagtggccac gcccagcgcc tcggccactt cgcgatacgg tcgcgccggt gtttcgaaaa
     6961 agagcatctg caccatcaca cggcagcgct cgggcagcga tgcgatgccg tcgcgcagcg
     7021 cctggctccg ttggcagtgc tcgatgagcg cgtcgggtgg ctcttcggaa gccgccgctt
     7081 cggtgccgcc gccctcgtcg gccacgtagc gttgattgcg gtgcctccac ttggcggcct
     7141 tgcgcgccgt cgttctgatg agccacgccg gcagcgcctg tggatcgcga agcctcggca
     7201 gctcgacgat cagatcgagg cacacggcct gaaagatgtc cgccgcttcc tcgcgaccga
     7261 gtccctgccg cagcggaatc gagaagatca ggcggctgta gcggtcgacg agcgctcccc
     7321 acgcgtcttc ctctcccgac aggcacgccg caaccagctc gcggtcagag cgcgttccct
     7381 gctggaacgg cgggaggttg gccagcgcat tcatcgacgt tggctcccat cgggctggat
     7441 gaagagccgg tccacgttcc acagcgtgta gtgatcgagc acggacggct ggaagttgcc
     7501 gaggtcgggc cgcgcgccga ccaggatgtg cggcgccgcg agaaagatga acggaagctg
     7561 ctcggccacc agctcctgca cgcggtcgaa caggcgcttc cgctcgcggg agtcgagggt
     7621 cacgagctgt tgcgccatca ggcgatcgac ctccgcctcc caggccgtcg ccggcgtctt
     7681 ctgcccgagg cgccagaggt gattgctccc ggtggacagc cagacgctca tctcgctgtt
     7741 cgggtcggca tcgccgccgc cgaggccgag aatcgacgcg tcgtagtcga aggtctgaaa
     7801 cacgcgatcg accagcgccc ggaactcgag aggcacgacc tggacgtcca tgccgagctc
     7861 cttcaggtcg gcctggacga gcgtcgccat ctcggtgcgc tgcgtgttgg aggagctcgt
     7921 gacgatggtg aaggcgacgc gctgaccctg ctggtcgagg agcgcgccat ctgggcgcca
     7981 ggagaagccg gacgtcttca ggatcgcgcg cgcctcgctc accgagcgcg cgggacgcgc
     8041 gatcgaggcg ttcacccagc gtcggttccc gggactcacg ttgccccaga gcggcaccgc
     8101 ccgtccccga tagaccagcc gcgcgatgct ctcgcgatcg acggcgcgcg agacggcgcg
     8161 ccggaaaccg agatcgccaa accagcgctg acgggactcg atctgcggca ggcccttgcc
     8221 gctcagatcg ttctggttga agacgaggaa gttgtattcg aggccggggc cgaggtcctc
     8281 gagtcgatga cgggcggatc cgtttcgggc gagcgcggtg aagttgtcgg cgctcgcccg
     8341 gttgaggatg tcgctttctc cgctctggaa gcggatgatc tgggcgtcct cggtgcccgc
     8401 gaacagaaac acgatctcgt ccatgtacgg cagccggcgc ttcgcggcgt cggccttcca
     8461 gtagaacggg ttgcgctcga ggaccagtcg ttgtccggcc gtgtattcct tcaatcggta
     8521 cggaccaagt cccgcgatgg cgcccgccga cgtcgtcaac ggccaggctt ctgccagcgc
     8581 gcctcgagcg taggcgccgg ccaggaggtg ctccggcagg atggcgatgc tgtcgaagag
     8641 ccgctccgcc gcggcatagg gctgtgccag cgcgacgcgg accgtgtgcg cgtcgacctt
     8701 ggtcacgcgg acgggctgac cgccgacgat gagcaggtcc cgctgcggcg cgcccgtgcg
     8761 ctcgtccagc atcgcgcgga acgtaaacac cacgtcgtcg gcgtcgaatg gctgtccatc
     8821 cgagaagcgg atgcccttgc ggagagtcag ggtgtactca cgcccgtcgg tcgagcgcgt
     8881 ccagcccctg gcgagcgcgg cctcggtcaa ctgcgtcgag cggttgatgt gaatcaggtc
     8941 gcctgtcatg cggccgatca cctcgcgcga cggcgcatcg gcggcgatgg ctgggttgaa
     9001 cgtgcgaggt tcggctcgaa gggccacgac aagccgacga ccgtagtggc cggagccgtc
     9061 gcgttcgacg aggacttctt cgcccgccgc gatcggcagc ggctgcgcgg ccgctcctgc
     9121 cggaagcgtt gcgaagagcg cggcgaggag gcacgacacc ccgagtcgca tggacgggcc
     9181 tttcctcatg tcgtcaccag ccggatgcgg ccgtgaacgg cggacgtcac ggcatggtag
     9241 gtcaccgccg taacgaccag tgccacagcc ggcgcgaaca tccaccagta cgacgtcagg
     9301 acgtcgtact gctgcaggct tccgagcatc gtgccccagc tcggcgtggg ctccccgacg
     9361 ccgagcccga ggaacgacaa ggtcacctcc gcaaggatgt actgcgggac gagcagaccg
     9421 gcctgcgtca gcgccacacc gaaggtgtgc gggagaaggt gacggcgcag caggtacagc
     9481 ggctttgcgc cgaagccgcg ggcagcgaga acgtaggtgc gctctcgggc actcaggacg
     9541 acgccccgaa tcaggcgtgc cggcctggcc cacccgacga cgcccagaac cgtcaccagg
     9601 agcagaaacg tggcgcgcgt gtcgatgtgc agcggcaacg cggcacgcgc cgcaaagagg
     9661 aggtacaacc acggcacggc catgaacagg ttggccgccc acatcacgcc cccgtcgatc
     9721 cggcctccgc tgaagccggc gaccgtgccg aggaccatac caatcagaag cgcagagaga
     9781 gtggccagca gacccgcccc taccgatacc cggcttccat gaagcagccg cgagagcagg
     9841 tcgcgcccga agccatccgt cccgaggagg aagagacggc ccggcgtctc gacaccgacg
     9901 agatgcagat tgctttcgag tcccggcagc accgaataac gcgcgccgcg caccatcatc
     9961 tggacgggga aacgtcgtga ccggtcctcc atgtagccgt cggcggttgc gggatccggt
    10021 accaaggcgt acacgaacgg ccggggatgg aagcggccgt ccgcgtcgat cacgtgcagc
    10081 cggaccggcg gtgcaaacgg aagagcccgt gcctgatcca ccgggtcgta cggcgcgacg
    10141 aatcccgcca ggagcaccag aagatgcaga gccgccaaca ccacgacgag agcccgtgcc
    10201 cgtccccgcg tcatgcgcga atcctcggat cgatcccgac gagcgtcagg tcggcggcga
    10261 gattcccgag caccagcagc ccggtggcca gcatcgaggc gctgagtact acatggacgt
    10321 ctttggccag cagcgcctcg acgagcagcg ggccgaggcc gggccagctc atgacgacct
    10381 cgatgagcag cgacgcgctg agcagcgtgg cgattgacag tccggcgagc gacaccagcg
    10441 ggttggccgc cagcggcagg acgaagcgca gcgtgaggcg cgacggtctg atgccgaggc
    10501 cacgggccgc ctgcaccgca ggagccccag ccgcttcgat catgctggcc cgaacgtgcc
    10561 ggaacaccgc gggcagcgtc gtcaccacca gcgcgcagag aggcagaaac gcatgggcgg
    10621 cgaaatcccg gacacgggcc gtgacgctca tgtcttcggc gccaggagac agcattcccc
    10681 ccgtgggaaa gagcccggtt cgcaaggcca gcaggagcag cagcagcgca acgagcagat
    10741 cgggcagcgc cagcagcgta gacgtcgcgc ccccgcagag acggtccggc cagcgcccgc
    10801 ggcggcgcgc ggcccagatc ccgagcggca gcgcaagccc ccaggtcacg aggagcgcgg
    10861 gaacggtcag cagcagcgta ttacccgccc gcacccagag aaccgatgcc accggcgcgc
    10921 ggtgcgccag cgagaagccc agatcgccgc tcacgaacga ctgcagccat tgccagtacc
    10981 gagccagcgg cgaccgggtc aggccgtact ggttccgcag tgcctcgacc gtctcgggtc
    11041 cgactcgggt ctcggcccgc agatcggtga aaaagtctcc gggcgccagt tccagcagca
    11101 ggaatgaaaa caccgacacc gaggccagcg tgatcaggct gcgggcgacg tagcggaaaa
    11161 cgactctcac gcggtcgccc ctatcggagc aaggtccggc gacagcgtgt caccgcgatg
    11221 ccccgcgtat gccctgtaga gaacctcgtc gggcacgcct cgctcgacga tgcgcccctc
    11281 gcgcatgagc gcgacctcgg aggcgaactc ggcgagcagc gcgagatcgt gcgagatgca
    11341 cacgaacgtc aggccgcgcg ccgcctggag atgcatcaac acgccgagga tgcgcgatcg
    11401 tgtcgcctgg tcgagtccgg agaaggactc gtcgaagatc agcacggcgg gatcgacggc
    11461 cagcgcgcgg gcgatcgcca gacgctggcg ctcaccgccg ctgaactctt cgggccggga
    11521 ctcgaggcgg tccgatggca gttccaccag cgccatcagt tcggcggcac gatgccggcg
    11581 ctcgccggcc gtcccgatgc cctggatcac caacggctcg ctgacgatct ggccggcggt
    11641 gaagcgcggg ttgagcgcag ccgccgagtc ttgaaagatc acttgaagct gatttcgata
    11701 cggcaggagg gcacggccgc gaagagccgc gatgtccgtc ccgttgatcc ggatgtggcc
    11761 ggcgtcggct ggttccagcc cagccatgca ccgtgcgagc gtggacttgc cgcatccgga
    11821 tcgccccgtc agacccaggg tggatccgcg ttcgatgtcc atgtcgacgc cgcgcagggc
    11881 ctcgacctca tgccggcgtc cggagaacat ccggcggtgc gtgtaggtcc gtctcagacc
    11941 tcgcagctcg acgagtggct cccccgtccc cgtccctcgc tcgccccctt cgactcgggg
    12001 cgaggtcctg cgcacgcggg tcgtcgtcct cgtcggtact tctggtcggt cgtcgatgcg
    12061 cccggccgtc atcttgatca cgcgcgtggc agtcgtcgcg agcacatccg cactgtgcgt
    12121 gatcaacaga aacgagatgc caagctgttc gttgagcgtg cgaatcaggc cgagcacgtc
    12181 cgccctgaca tccgcgtcga gcgacgccac cggctcatcg gcaatcacga gccgaggccg
    12241 gcagacgatg gcctgggcga tcaggacccg ctggcgctcc cctccgctga gctcgtgagg
    12301 gtaggcgtcg aagattcgcg gcaggttctc gcccaagccg acctcggcca tagcggcgcg
    12361 ggcacgctcg agcgccgcgc cgcgatcgca ccgttcgtgc gcgcgaacca cctcggcgat
    12421 ctgcacgccc gccctgagta gtggattgag ggccagtgac gattcctgga agacgatgcc
    12481 caactcggcc ccacgaaccg tctgcagatc gcgatcggtg aggcccgtca gctcacggcc
    12541 ttgaaactgg attgatcctc gaagttcggc ggccggcggc agaaggccca tgagggccag
    12601 ggcgagcgtt gtcttgccac atcccgacgg gcccgacagg cccacgatct cgccgggccc
    12661 gagagacatg ctgacgccgt cgatcgctgg cggtcgatcc tcgtaacgaa ccgacaaatc
    12721 agtaatttgc aacaagggca cacgacacct ggtgcgccat ggccgagcaa gtccactgcc
    12781 accgaagctt accaaaattg ctcagcacgc gcaggcctgg agaccggtcg tgtgccacgt
    12841 cggcacaagg cctgcttgaa gtcgagaact tctgaccaaa tctggttctg ggggctataa
    12901 acgcttcgta gggtgagaac ccgagcgcga gggccttcgg tccgctcgag cgtcccccgt
    12961 gttatcttgc ccgccatgac gagagcgacc agggtggtct cgtgtctggc cgtgcttgcc
    13021 tcgtgggcgg ccgccctgaa ccacccggcg ttggcccacc attcctttgc gatgttcgac
    13081 acggccaagc gggtcacgct cgccggaacc gtcacggcgt tcgagtggac caatccgcac
    13141 gcctacatcg agatcgacgt cgaggagtcg gggcgggtca ggcactggag catcgagctc
    13201 ggaagcacca gcatcctcat gcagggcggg tggaagttca acgacctgaa gcg
//
LOCUS       DBNGSH010000337         2927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3932318, whole genome shotgun sequence.
ACCESSION   DBNGSH010000337 DBNGSH010000000
VERSION     DBNGSH010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3932318"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /locus_tag="AC16CY_07915"
     CDS             <1..1868
                     /locus_tag="AC16CY_07915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3353750.1"
                     /translation="PPAAPAAPSGPNVQENRGPAGPIRTFQDLLTSAHDEKLFEYYAT
                     SQLAFVDAATGRRSPVGKPGIVSGAASPDGQYILVSRVKRPFSRLLTWGQFPRDVEVW
                     NRRGDTVRTVADVPMGDTVPINGVITGPRAQRWAPLEPATLIWAEALDKGDIKNKVPH
                     RDRIMSLGAPFSGEPSEVAKTQYRYGGASWTDAGTILLTENDRDTRTTRTWVLAPPKP
                     RSGEGGLNSRWGEPRKLWDRKQQDSYGDPGSPVFRPGRPTVLQVGDAIYLNGLGASPD
                     GDRPFLDRLNLRTLATERLFRSDASSFESIVGLLDEGGTRVLTRRETKTLPPNLVIRD
                     LRAGTEQPATRFADPHPQLTKAMADRLFVTYKRKDGVGLSGTIYLPVGYQKGQRVPML
                     LWAYPQEFVDPDAASQVVGSPNRYTGVAGSSHLLLLMQGYAIFDGPTMPIVGPGETAN
                     DKYIEQLVASAEAAVDKAVELGIADRSRIAVGGHSYGAFMTANLLAHSDLFTAGIARS
                     GAYNRSLTPFGFQAETRTFWEVPDVYAKMSPFWYANKIQEPILMIHGEADDNSGTFPI
                     QSERFYMALKGHGATVRYVTLPHEAHGYAARESNMHVVAETLTWLDRYTKNRQER"
     gene            1908..2300
                     /locus_tag="AC16CY_07920"
     CDS             1908..2300
                     /locus_tag="AC16CY_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3353751.1"
                     /translation="MATIEKFCASGVFDPATYSQGIKVTNAQTILFLSGQVAYTADGG
                     VAFRGDFKGQARGAFEAIKALIESQGGTMANIVKFTTYVTDMRYRADLVPLREEFLGK
                     KGPASTLVEIPALAHPDWMIEIEAIAVL"
     gene            complement(2310..>2927)
                     /locus_tag="AC16CY_07925"
     CDS             complement(2310..>2927)
                     /locus_tag="AC16CY_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353752.1"
                     /translation="GLAAGAAVKPFPPGRRDGFFESYMSEIAAYKLDRMLDLNMVPPT
                     VERRYEGKEASAQLWVENVIMLSDVQKKGLKDPNPLRWNQNLHRAQVFDDLVGNIDPN
                     AGNWLFDPAWNFYKIDCSRCFTDTMKMPFEVKKAIRKIDRPFFDRLKALDRDAVRKEI
                     GDLLTENNAFPRLFSRRDSIVRDFEELAKQNGDAQVFEPWTGQGQ"
BASE COUNT          524 a          976 c          965 g          462 t
ORIGIN      
        1 gcccgccggc ggcgcccgcc gcgccgtcag gcccgaacgt tcaggagaac cgcgggcccg
       61 ccggcccgat tcgcacgttc caggatctgc tgacgagcgc gcacgacgag aagctgttcg
      121 agtattacgc gacgtcgcaa ctcgcattcg tcgatgcggc aaccgggcgc cgctcccccg
      181 tcggcaagcc cggcatcgtc agcggtgcgg cgtcgccgga tggccagtac atcctcgtgt
      241 cgagagtcaa acgcccgttc tcacgcctgc tcacgtgggg acagtttccg cgcgacgtcg
      301 aggtctggaa tcggcgcggc gacacggtgc gcacggtcgc cgacgtgcca atgggcgaca
      361 ccgtgccgat caacggcgta atcaccggac ctcgggcgca gcgctgggcg ccgctcgagc
      421 ccgccacgct catctgggcc gaggcgctcg acaagggcga catcaagaac aaggtgccgc
      481 atcgcgacag gatcatgtcg cttggcgcgc cgttctcggg ggagccgagc gaagtcgcga
      541 agacgcagta tcgctacggc ggcgcgagct ggaccgacgc gggaacgatc ctgctgaccg
      601 agaacgaccg cgacacgcgc accacgcgga cctgggtgct tgccccgccg aagccgcgca
      661 gcggcgaagg agggctcaac agccgctggg gcgagccgcg caagctgtgg gaccgcaaac
      721 agcaggacag ctatggcgac cccgggagcc cggtcttccg tcccggccgg ccgacggtgc
      781 tgcaggtcgg cgatgcgatc tacttgaacg ggctcggcgc gtcgccggat ggtgatcgtc
      841 cgttcctgga tcggctgaac ctgcggacgc ttgcgaccga gcgtctcttc cggagcgacg
      901 cgtcgagctt cgagtcgatc gtcggtctcc tcgacgaagg cggaacgcgc gtgctcacgc
      961 gcagggagac aaagacgctg ccgccgaacc tcgtcatccg cgaccttcgc gccgggaccg
     1021 aacagcccgc gaccaggttc gccgacccgc atccgcagct cacgaaggcg atggcggacc
     1081 ggctgttcgt gacctacaag cggaaggacg gcgtcggcct gagcgggacg atctatctcc
     1141 cggtcgggta ccagaagggc cagcgcgtcc cgatgctgct gtgggcctat ccgcaggaat
     1201 tcgtcgatcc ggacgcggcc agccaggtcg tcggatcgcc gaaccgctac acgggcgtcg
     1261 cgggatcctc gcacctgctg ctgctgatgc agggctacgc gatcttcgac ggccccacga
     1321 tgccgatcgt gggacccggc gagacggcaa acgacaagta catcgagcag ctcgtcgcga
     1381 gcgcggaggc ggccgtggac aaggcggtcg agctcggcat cgccgatcga agccgcatcg
     1441 ccgtcggtgg ccacagctac ggggcgttca tgacggcgaa cctgctggcg cactcggacc
     1501 tgttcaccgc cggcatcgcg cgcagcggcg cgtacaaccg ctcgctgacg ccgttcggct
     1561 tccaggccga gacgcgcacg ttctgggaag tgcccgacgt ctacgcgaag atgtcgccgt
     1621 tctggtacgc gaacaagatc caggagccga tcctgatgat ccacggcgag gccgacgaca
     1681 actcgggcac gtttccaatc cagtccgagc gtttctacat ggcgctcaag gggcatggcg
     1741 ccacggttcg ctacgtcacg ctcccgcacg aggcgcacgg atatgccgcg cgcgagtcga
     1801 acatgcacgt cgtggccgag acgctgacct ggctggatcg gtacacgaag aaccgtcagg
     1861 agcgatagca gggggcgggg ggcaggggga agggggcagg aagcgacatg gccacgatcg
     1921 agaagttttg cgcgtcaggc gttttcgacc ccgccaccta cagccagggc atcaaggtca
     1981 ccaacgccca gacgatcctg tttctctcag ggcaggtcgc ctacaccgcc gacggcggcg
     2041 tcgccttccg cggcgacttc aaggggcagg cacgcggcgc gttcgaggcg atcaaagcgc
     2101 tcatcgagtc ccagggcggc acgatggcga acatcgtcaa gttcacgacg tacgtgaccg
     2161 acatgcgcta ccgcgcggac cttgtcccgc tgcgcgagga gtttctcggg aagaaagggc
     2221 cagcctcgac gctcgtggag atcccggcgc tggcccaccc ggactggatg atcgagatcg
     2281 aggcgatcgc ggtcctgtaa cgtggacgcc tactgaccct gacccgtcca gggctcgaag
     2341 acctgagcat cgccgttctg cttggccagc tcttcgaagt cacgcacgat cgaatcgcgg
     2401 cgcgagaaca accgtggaaa ggcgttgttc tccgtcagga gatcgccaat ttccttcctc
     2461 accgcatctc ggtcgagcgc cttcagccgg tcgaagaacg gccggtcgat tttcctgatc
     2521 gccttcttca cctcgaacgg catcttcatg gtgtcggtga agcagcggga gcaatcaatc
     2581 ttgtagaagt tccacgccgg gtcgaacagc cagttccccg cgttgggatc gatgttgccc
     2641 accaggtcgt cgaagacctg cgcccggtgc aggttctgat tccaccggag gggattggga
     2701 tccttgaggc cctttttctg gacgtcgctc agcatgatga cgttctccac ccagagctgg
     2761 gccgacgctt ctttcccttc gtatcgccgc tcgacggtcg gcggcaccat gttcaggtcc
     2821 aacatcctgt cgagcttgta agcggcgatc tccgacatat agctctcgaa gaagccatcc
     2881 cgccgtcccg gcgggaacgg cttgaccgcc gcaccggccg ccaggcc
//
LOCUS       DBNGSH010000338         5073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2065034, whole genome shotgun sequence.
ACCESSION   DBNGSH010000338 DBNGSH010000000
VERSION     DBNGSH010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2065034"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="AC16CY_07930"
     CDS             complement(<1..713)
                     /locus_tag="AC16CY_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353753.1"
                     /translation="MRLALVFAVLATAAASAQGPPGGRGAAPPRSAREAAPVDFTGYW
                     VSVVTEDWRWRMVTPAKGDFAAVPLTPAARKLGLAWDPARDEAAGEQCKAYGAAAIMR
                     VPGRLYITWQDDNTLQIQTDAGTQTRLLRFRGGAADAQPSWQGHSSAQWEGLVRGSGS
                     PDFLPIGLNPREGTRGRSLEVVTTNLRPGYLRRNGVPYSARTTLREYFDLSTERNGDI
                     WFTVTSIVEDPEYLTEPFVT"
     gene            complement(710..1684)
                     /locus_tag="AC16CY_07935"
     CDS             complement(710..1684)
                     /locus_tag="AC16CY_07935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353754.1"
                     /translation="MSGPFITPLRALTVSIALLAAGLAGAEGRAQQGGAPTGAAGLLR
                     VQGNIHMLVVGGMNIAVQVGSDGVLLVDAATEASAQQVIGAIRTLSDKPIHTIVNSHV
                     HADHTGGNDTLVKMRGTGASQPVRVIAQDNVLRRLQSGKTPVAGFRLNAVITVPINST
                     YDTPSRDFYLNGESIIVYHAPAAHTDGDSIVHFRGSDVLAVGDLYSPDRYPVIDIANG
                     GSVQGLIAALNKVLELTVPARFQEGGTYVIPGHGRLSDEADVVEYRDMVTIIRDRVQD
                     LISKRRTWDQVKAARLSRDYDPEYGTPTGPSSPDQFVEAIYRSLGGQL"
     gene            complement(1681..2604)
                     /locus_tag="AC16CY_07940"
     CDS             complement(1681..2604)
                     /locus_tag="AC16CY_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353755.1"
                     /translation="MTWKAVNATAVVLAALCGIAAPAQAQVDFTGQWAPLYQEDTIER
                     VPGPELGDYTGLPLTEAGRMRADTWDADRISVVQEYQCRPHSADYSLRGLAPMRVWAD
                     YDQATQRIVAFHTHLGAYENKRTIYLDGRLHPPEYAAHTFQGFSTGVWEGNTLTITTT
                     HLKANYLRRNGVPRSAKARLTEHWNVHGAYLTITSVLEDPVVLTEPLVRSQSWFLDPG
                     QRAGLFPCEYVPEVPAPPGTVPHHLPGANPNLKEYLDWYAVPAEAARGGAETLYPEYQ
                     LKLQKIYKPKDRCERYCNCVGFAGCLSITGP"
     gene            2733..3494
                     /locus_tag="AC16CY_07945"
     CDS             2733..3494
                     /locus_tag="AC16CY_07945"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3353756.1"
                     /translation="MLLRVLVIAAIGAAVAVGLFFLFGRQPTQPSTSGARESARTRPA
                     TGARPTGTPEAPAAASPAAAGSRDARRPPAPAAAEPESAPASAELGTLRIDSDVPGAQ
                     VFIDRQFVGTTPMTAERVSPGTHQLTLAAEGFDGIRQTIDVEPGPRELTFRFREVRLD
                     ATLAVVHKHRIGSCRGRLVADVDGLRYEAAEGDDSFSSPLAGLETFQVDYLEKNLRIA
                     LRKGRTYNFTDPDGNADRLFVFHRDVENARRRLGD"
     gene            3499..4572
                     /locus_tag="AC16CY_07950"
     CDS             3499..4572
                     /locus_tag="AC16CY_07950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3353757.1"
                     /translation="MPEFDSKEAEAFAGRLLSALNDGALCLMASIGHRTGLFDVMRNQ
                     PPQTSDEIAARAGLNERYVREWLGAMVTSGVVSVDPQTIRYQLPPEHAAYLTRAAGAD
                     NLAVFAQYVPMLANVEDDIVECFRAGGGVPYERYPRFHAVMAEDSGLSVLSSLESHIL
                     PLVPGLADRLARGMRVLDAGCGSGRIINRLAARFPKSRFTGMDLSQEAIRAGREEASA
                     KGLKNVEFVSVDLSGFDRTAEPESFDLITTFDAVHDQSKPLNVLKGIHRALARDGVYL
                     MQDIRGSSHVARNIGHPIGTFLYTVSCLHCMTVSLAQGGEGLGAMWGEEKTREYLERA
                     GFGSVEKHELAHDIQNNWYVVRK"
     gene            4590..>5073
                     /locus_tag="AC16CY_07955"
     CDS             4590..>5073
                     /locus_tag="AC16CY_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353758.1"
                     /translation="MRTTTTIRPLRPSPLAWLIAALLVVLAADAAPAQTRGTGSGASP
                     GAPRAWTPPMTSDGQPDLQGVWADLSATPLERPRALAGRTSLTDAEVAQLKARAERLW
                     NNPASDFVGGDNLFLAVLSNLDTVDNPNATGNSLNMVKREFDNRTSLITDPADGRLPA
                     L"
BASE COUNT          789 a         1689 c         1726 g          869 t
ORIGIN      
        1 gtcacgaacg gctccgtcag gtactccgga tcctcgacga tcgaggtgac cgtgaaccag
       61 atgtcgccgt tccgctcggt cgagaggtcg aagtactcgc gcagcgtcgt gcgcgcgctg
      121 tagggcacgc cgttcctccg aaggtagccc ggccgcaggt tcgtcgtcac gacttcgagc
      181 gaacggccgc gtgtcccttc gcgtggattg aggccgatgg gcaggaagtc aggcgagccg
      241 gagccgcgaa ccagtccctc ccactgggca gaggaatgac cctgccacga cggctgagcg
      301 tcggccgcgc cgccccggaa ccgcagcagg cgggtctgcg tgccggcgtc ggtctgaatc
      361 tgcagcgtgt tgtcgtcttg ccacgtgatg tacagccggc cgggcacgcg catgatcgcc
      421 gccgcgccgt atgccttgca ctgctcgccg gcagcttcgt ccctggccgg atcccaggcg
      481 agcccgagct tgcgcgcggc gggcgtgagc ggcacggccg cgaagtcgcc tttcgctggt
      541 gtcaccatcc gccagcgcca gtcttcggtg acgaccgaga cccagtagcc cgtgaagtcc
      601 accggcgccg cctcgcgcgc cgagcgaggc ggcgcagcgc cacgtccccc cgggggccct
      661 tgggcggatg cggccgcggt tgccagaacg gcgaagacga gcgcgagcct cacaactgtc
      721 ctccgaggct ccgatagatc gcctccacga attgatcggg tgaagacgga ccggtcggcg
      781 tcccgtactc ggggtcgtag tctcgtgaca gtctcgccgc tttcacttga tcccacgttc
      841 gtcgcttgga gatcaggtcc tggacgcgat cgcggatgat ggtgaccatg tcgcggtact
      901 ccacgacgtc cgcttcatcc gacagacgcc cgtgtcccgg gatgacgtac gtcccgccct
      961 cctggaacct ggcgggcacg gtcagctcga gcaccttgtt caaggcggcg atgaggccct
     1021 gcacggatcc cccgttcgcg atgtcgatga ccggataacg gtccgggctg tacaggtcgc
     1081 cgacggcgag cacgtcggag ccgcggaagt ggacgatgct gtctccgtcg gtgtgcgcgg
     1141 ccggcgcgtg atagacgatg atcgattcgc cgttcaggta gaagtctcgg ctcggcgtgt
     1201 cgtacgtgct gttgatcgga acggtgatga ccgcgttcag gcggaacccg gcgacgggcg
     1261 tcttgccgga ctgcagccgc ctgagcacgt tgtcctgcgc gatcacgcga acgggctgcg
     1321 acgcgcccgt gccgcgcatc ttcaccagcg tgtcgttgcc gccggtgtgg tcggcgtgga
     1381 cgtgtgagtt gacgatggtg tgaatcggct tgtcggacag cgtgcggatc gccccgatga
     1441 cctgctgagc cgacgcctcg gtggcggcgt ccacgaggag gacgccgtcg ctgccgacct
     1501 ggacggcgat gttcatccct cccacgacca gcatgtggat gtttccctgc acgcggagca
     1561 gcccggctgc gcctgttggt gcgccgcctt gctgagcgcg tccttccgcg cccgcaaggc
     1621 ctgcggcgag cagtgcaatc gacaccgtca gcgcgcgaag cggcgtgatg aaaggtccgc
     1681 tcatgggccg gtgatcgaca ggcagccggc aaacccgacg cagttgcagt agcgctcgca
     1741 gcggtccttc ggcttgtaga tcttctgcag cttcaactgg tactccgggt aaagcgtttc
     1801 ggccccgccg cgcgccgctt ctgccgggac cgcataccag tcgaggtatt ccttcaggtt
     1861 tggattggcg cccggcagat gatgcggcac cgtccctggc ggtgccggaa cctcgggcac
     1921 gtactcacac gggaagagac cggcgcgctg accgggatcg aggaaccacg actggctgcg
     1981 gaccagcggt tcggtcagca cgaccgggtc ctcgagcacg ctggtgatcg tcaggtaggc
     2041 gccgtgcacg ttccagtgct cggtgagccg ggccttcgcg ctccggggca cgccgttccg
     2101 ccgcaggtag tttgccttga ggtgcgtggt ggtgatcgtg agggtgttgc cttcccagac
     2161 gcccgtggaa aatccctgaa acgtgtgcgc cgcgtactcc ggcgggtgca ggcggccgtc
     2221 cagatagatc gtccgcttgt tctcgtaggc cccgaggtgc gtgtggaaag ccacgatgcg
     2281 ctgcgtggcc tggtcgtagt ccgcccacac gcgcatcggg gcgagccctc gcagcgagta
     2341 gtcggccgag tgcgggcggc actggtactc ctggacgacg gagatccgat cggcgtccca
     2401 ggtgtcggcc cgcatgcgcc cggcttcggt cagcggcagt ccggtgtagt cgcccagttc
     2461 cggccccggc acgcgctcga tcgtgtcttc ctgatagagc ggcgcccact gacccgtgaa
     2521 atccacctgc gcctgcgccg gcgcggcgat cccgcacagc gcggcgagca cgacggccgt
     2581 ggcgttcacg gccttccacg tcatcgaaat tctcctccgt cgcggatgcc tatggacgcg
     2641 acgctccgcg gttgctcatt ctactgcggg gctcgagtga gcgccgtcgt agcgtagtgt
     2701 ccggcgtcag ccggaccgca tagaatcgca tcgtgctcct tcgcgtgctc gtcattgccg
     2761 ccatcggcgc cgccgtcgcc gtcggcctgt tttttctatt cggtcggcag cccacgcagc
     2821 cgtccacttc gggtgctcgc gagagcgctc gcacgagacc ggcaaccggc gctcgaccca
     2881 ctggaacgcc ggaagcgccc gcggctgcgt cgcccgccgc ggccggatcg cgtgacgcga
     2941 ggcggccgcc tgctcctgcc gcggccgagc ccgagtcagc gccggcctca gcggaactgg
     3001 gcacgctccg gatcgattcc gacgttcccg gcgcccaggt gttcatcgac cggcagttcg
     3061 tcggcaccac tccgatgacg gccgagcgag tgtcgccggg cacgcatcaa ctaactctgg
     3121 ctgcggaagg gttcgatggc atcaggcaga cgattgacgt cgagcccggg ccccgcgagt
     3181 tgacgttccg ctttcgcgag gtgcgcctgg acgccacgct ggcagtcgtg cacaagcatc
     3241 gcatcggttc gtgccggggt cggctcgtcg cggacgtgga cggcctccgc tacgaggcgg
     3301 ccgagggcga cgattcgttc tcatctccgc tcgccggtct ggagacgttt caggtcgact
     3361 acctcgagaa gaacctgcgc atcgcgctgc ggaagggacg gacctacaac ttcaccgacc
     3421 ccgacggcaa cgccgatcgc ctgttcgtgt ttcatcggga tgtggagaac gcgaggcgaa
     3481 ggctgggaga ctgacgacat gccggagttc gattcgaagg aagccgaggc gtttgccggc
     3541 cgcctgctga gcgcgctcaa cgacggcgcg ttgtgcctga tggcctccat cgggcatcgc
     3601 acgggtctct tcgacgtgat gcgcaaccag cctccgcaga cgtcggacga gatcgccgca
     3661 cgggcggggc tcaacgagcg ctacgtgcgc gaatggctcg gggcgatggt gacctcgggg
     3721 gtcgtcagtg tggatccaca gaccattcgc taccagctgc cgccggagca cgcggcgtat
     3781 ctcacgcggg cggcgggagc cgacaatctc gccgtgttcg cgcaatacgt cccgatgctc
     3841 gccaatgtcg aggacgacat cgtcgagtgc ttccgtgccg gcgggggagt tccctacgag
     3901 cgctacccgc gattccacgc ggtgatggcc gaggacagcg gcctgtccgt gctgtcgtcg
     3961 cttgaatccc acatccttcc cctcgtcccg ggcctggccg atcgcctggc ccgcgggatg
     4021 cgggtgctcg atgccgggtg tggcagcggc cgcatcatca accgcctcgc cgcacggttc
     4081 ccgaagagcc gcttcacggg aatggatctg tcgcaggagg ccatccgggc cgggcgtgag
     4141 gaagcgtcgg ccaagggact gaagaacgtc gagttcgtct ccgtcgatct cagcgggttc
     4201 gaccggacgg ccgaacctga gtccttcgac ctgatcacca cgttcgacgc cgtgcacgat
     4261 caaagcaagc cgctcaacgt cctgaagggc attcaccgcg cgctggctcg agacggtgtc
     4321 tatctgatgc aggacatcag gggatcgagc cacgtcgcca ggaacatcgg ccatccgatc
     4381 ggaacgttcc tgtacacggt atcgtgcctc cactgcatga ccgtctcatt ggctcagggg
     4441 ggcgaaggac tgggcgcgat gtggggtgaa gagaagacgc gcgagtacct cgagcgggcg
     4501 gggttcgggt ccgtggagaa gcacgaactg gctcacgata tccagaacaa ctggtacgtg
     4561 gtcaggaaat gaggctctgc cgcagtcaaa tgcgcaccac caccacgatt cgcccgctcc
     4621 gcccgtcgcc cctggcgtgg ctgatcgcgg cgctcctcgt cgtcctggcg gctgacgccg
     4681 ctccggctca gacacgaggg acggggtccg gggcttcgcc gggcgcgcca cgggcctgga
     4741 cgccgcccat gacctcggat ggtcaaccag acctgcaggg agtctgggcc gacctcagcg
     4801 cgaccccgct cgagcggccc cgggcgctgg cgggaaggac gtcgctcacc gatgcagaag
     4861 tggcgcagtt gaaggcgcgc gccgagcgtc tctggaacaa cccggcgagc gacttcgtcg
     4921 gcggcgacaa cctctttctc gctgttctgt cgaacctcga cacggttgac aatccgaatg
     4981 cgaccggcaa ttcgctgaac atggtgaagc gggagttcga caaccggaca tccctcatca
     5041 ccgatccggc ggacggacgc ctgcctgcct tga
//
LOCUS       DBNGSH010000339         7519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1895114, whole genome shotgun sequence.
ACCESSION   DBNGSH010000339 DBNGSH010000000
VERSION     DBNGSH010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1895114"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="AC16CY_07960"
     CDS             complement(<1..1088)
                     /locus_tag="AC16CY_07960"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR3353759.1"
                     /translation="MSASPALSRVEVDYSGGERLTMNMGPQHPSAHGVFRAILTLEGE
                     TVVGVDAVIGYLHRCHEKLSETLTYAQYPSIASKTDYVAAMTSEFAYVLAAEEIGKID
                     VPKRAQYLRVIAAELQRVASHLLWLGTWCMDMGGALGGGATIFLYCIRERELILDLFE
                     SLVGARLLYGFYQVGGTRYDLPAGWTAKCRETIDLIDTRIGEYESLLGDNPFFLARAR
                     GVGVISRELAEDVGISGPLLRGSGVPFDIRRSAPYSSYQDFTFNVPVSSEGDCYGRYL
                     VRMAEFRESIRIVRQVLDGLPEGPISSRPGLKSVAQIRIPKGESYARVEGPRGEVGCY
                     LIADGSAKPYRMKWRGASFSNLAVLPHV"
     gene            complement(1085..1537)
                     /locus_tag="AC16CY_07965"
     CDS             complement(1085..1537)
                     /locus_tag="AC16CY_07965"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3353760.1"
                     /translation="MTREQAAAAIAGQFSGAQVQAGASLVVTIAVDQWVAFARFAKET
                     LGCRFFAFSTAVDWKEQGLEVVAKVDNLDDGLPVVMKTRLGAGVSSCPSLVPVYRGAG
                     WMERECYDMFGIRFEGHPDLRRILLADDWVGHPLLKSYAVDTPHPPYR"
     gene            complement(1542..2039)
                     /locus_tag="AC16CY_07970"
     CDS             complement(1542..2039)
                     /locus_tag="AC16CY_07970"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3353761.1"
                     /translation="MHSFPGGFVVTTVADAFFNWARKSSVWPVTFGLACCAIEMMATF
                     ASRFDVERLGMVPWASPRHSDLMVVSGTVTIKMAPMLERIYDQMPDPKWAVSMGSCAN
                     SGGPFRHGYHVVKGVDRVIPVDVYVPGCPPPPESLLNGLLLLQDQIAHFRRTGRRAEP
                     NPKVD"
     gene            complement(2171..2524)
                     /locus_tag="AC16CY_07975"
     CDS             complement(2171..2524)
                     /locus_tag="AC16CY_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3353762.1"
                     /translation="MTDYLPVVIYLGIIVAFAVVSLGAARVLSPGRPFAAKLQSYECG
                     AEPIGQAWVQFPVGFYLVALVFIVFDALAVFLFPWALAIRGLGAWGILAMLMFLSILL
                     LGWIYAYREGILEWK"
     gene            complement(2570..3616)
                     /locus_tag="AC16CY_07980"
     CDS             complement(2570..3616)
                     /locus_tag="AC16CY_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR3353763.1"
                     /translation="MSTPAQPAPGKKINRIGLEPTAYRGSKTTLCAGCGHNAITERII
                     DAFYELGVDPTQVLKLSGIGCSSKSPAYFLSQSHGFNSVHGRMPSVATGAVVANRKMI
                     AIGVSGDGDTGAIGIGQFVHLMRRNLPMVYIIEDNGCYGLTKGQFSPTADLGSKLKTG
                     VINDLPPIDTCLLAIELGASFVARSFSGDPKQLKAILKAALSHRGTAMIDVLSPCVTF
                     NDHDGSTKSYSYAKDHEEPLADVSFVPFFEEISVDYDPGTTTTVTLHDGSKLVLSKMT
                     EDYNPTDKLRALRLLHETSMRNEFATGILYVEPDKPDFIDLLGLVDEPLAHLDESRTR
                     PGKAALDEIMESFR"
     gene            complement(3687..5570)
                     /locus_tag="AC16CY_07985"
     CDS             complement(3687..5570)
                     /locus_tag="AC16CY_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3353764.1"
                     /translation="MSVTDAAVHEAATREPAARIVNDFAIQVATVNGSGSQTANLVLL
                     RSIFQMGVPVSGKNMFPSNIAGLPTWYTIRASARGYIGRKKEVDFLVALNPETAREDV
                     LTLAPGAAVVYDEPLKLRDLRGDLVFYPVPFDKIIGTITHDAKLRRLVRNMIYDGVLS
                     RLLAIDMGQMEKALRKQLGRKQKALEMNMAALKAGSDYAQATFTKQDPFVIEPMNKTT
                     GMILIEGNAAAAIGCMMAGVTFVAWYPITPSSSLPEALIDYLKKYRRDPDTGKTTYAV
                     VQAEDELAALGMVIGAGWTGARAMTSTAGPGVSLMAEFAGLGYYAEVPAVIFDIQRAG
                     PSTGLPTRTGQQDILSTAVLSHGDTNHPLLLPASVAECYTMAMEAFDLAEQLQQPIFV
                     MSDLDLGMNTWMAEPFEYPTRPFNRGKRLDKAALARIGQFGRYKDVDGDGIPYRTVPG
                     DGLPPYFARGSGHNEQALYSERADDYMRNLDRLSRKFQTAKTIMPRPEVELVEEGGIG
                     IIAYGTSHWAVVEARDQMKGEYGVTTSYLRLRAYPFSKELDDFIDRCDHVYVVEQNRD
                     AQMMSLLRIDLTPARVIKLRSIRHYAGLPIDGRSVTTDLLTQDGKEGLETPGSGLGTV
                     GAD"
     gene            complement(5714..6931)
                     /locus_tag="AC16CY_07990"
     CDS             complement(5714..6931)
                     /locus_tag="AC16CY_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIR3353765.1"
                     /translation="MIEDVGIVRQQRARLVPDLRGLPRPFWVLFAGTLVNRVGGFVVV
                     FLAIYLTDARGLTPVQAGTILTAYGLGAIGGGPIAGVLSDRFGRRPTLVASLMAGGAS
                     MLVLGLVTGYVTMIIASAVTGLLYEMYRPVVSATIADIVRPDDRPRAYSLIYWAVNLG
                     ASVAPLVGGLISARSYRALFAADAVTTALFGLIVWAALPETRPGDSADSDASGSGARS
                     ILKDHAFLAVCLLTLAFCIVFFQAFAALPIDVRAHGISPSGFGALMALNGVLIVLFQP
                     LAGELIRDRPRSSVLAAASLLLGIGFGMNAWIGSWPAYAASVVVWTLGEILFSPASTS
                     LIADLAPAHLRGRYQGAFAMAFTSGFALAPIAGGYVLDRAGAPWLWMACLAIGLAVGL
                     GFLTLGRMLRTVR"
     gene            6930..>7519
                     /locus_tag="AC16CY_07995"
     CDS             6930..>7519
                     /locus_tag="AC16CY_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353766.1"
                     /translation="MKTSALLVQVVLVASVTATPLSAQWINVPPSASPRTADGKPDLA
                     APAPRLPDGRPDLSGVWEPIDNRFVGNIAAGIGSENVPFQPWAKALFESRRDGSAARD
                     DPPASCLPQGVPRLGAAPAPWRVVQTPGLVVIVYEAFNLWRQVFLDGRQVAADANPTW
                     LGYSTGRWEGDALVVETRGFNGKTWLDQMGRPTTEAL"
BASE COUNT         1293 a         2521 c         2503 g         1202 t
ORIGIN      
        1 atgacgtgcg gcaggacggc cagattcgag aacgacgcgc cgcgccactt catgcggtac
       61 ggcttggcgc tgccgtcagc gatgagataa cagccgacct cgccgcgcgg cccttcgacg
      121 cgcgcgtagc tttctccctt gggaatccgg atctgcgcca ccgacttcag cccgggacgc
      181 gacgagatcg gcccttccgg caggccgtcg agcacctggc gcacgatgcg gatggattcc
      241 cggaactccg ccatccggac caggtagcgg ccgtagcagt cgccttcgct cgacaccggc
      301 acgttgaacg tgaagtcctg atacgaggag tacggcgccg atcgccggat gtcgaacggc
      361 actcccgagc cgcgcagcag cgggcccgag atccccacat cctcggccag ttctcgggag
      421 atgacgccga cgccccgggc gcgcgcgagg aaaaacgggt tgtcgcccag caggctttcg
      481 tattcaccga tccgcgtgtc gatcaggtca atcgtctcgc ggcacttcgc tgtccaaccg
      541 gcggggaggt cgtaccgggt gccccccacc tggtagaagc cgtacagcag ccgcgccccg
      601 acgagcgact cgaacagatc gagaatcagc tcgcgctcgc ggatgcagta gagaaagatc
      661 gtcgccccgc ccccgagcgc cccgcccatg tccatgcacc acgtgccgag ccagagcagg
      721 tgcgaagcga ccctctgcag ctcggccgcg atgacccgga ggtactgcgc ccgcttcggg
      781 acgtcgatct tgccgatctc ctcggcggcc aaaacgtagg cgaactcgct cgtcatggcc
      841 gccacgtagt cggtcttcga cgcaatcgac gggtactggg cgtaggtgag cgtttccgac
      901 agcttctcgt ggcagcgatg caggtaaccg atgacggcgt cgacgccgac gaccgtttcc
      961 ccctcgagcg tcaggatcgc gcggaacacg ccgtgggccg acgggtgctg cggccccatg
     1021 ttcatcgtga ggcgctcgcc tcccgagtag tccacctcga cgcgcgacag ggcgggtgag
     1081 gcgctcatct gtacggcggg tgcggcgtgt ccaccgcgta ggacttcagg aggggatggc
     1141 ctacccagtc gtcggccagc aggatgcggc ggaggtcggg atgtccttcg aagcgaatgc
     1201 cgaacatgtc gtagcactcg cgttccatcc agcccgcgcc gcggtacacg ggcacgagcg
     1261 acgggcacga cgacacgccg gcgccgagcc gcgttttcat cacgaccggg agcccgtcat
     1321 ccaggttgtc gaccttggcc accacctcga gcccctgttc cttccagtcc acggcggtgg
     1381 agaaggcgaa gaagcggcag ccgagcgttt ccttggcgaa gcgcgcgaag gcgacccact
     1441 gatccaccgc gatcgtaacg acaagcgacg cgcctgcctg tacctgggcg ccgctgaact
     1501 gtccggcgat ggccgcggcc gcctgctcgc gtgtcacggc gtcagtcgac cttcgggttc
     1561 ggttcggcgc gtcgtcccgt gcgccggaag tgggcgatct gatcctggag cagcagcagg
     1621 ccgttcagca gcgactcagg cggcggcgga cagccgggca catacacgtc caccggaatc
     1681 accctgtcca cccctttgac gacgtggtag ccgtggcgga acgggccgcc cgaattggcg
     1741 caggagccca tcgagaccgc ccacttcggg tcgggcatct ggtcatagat ccgctcgagc
     1801 atcggcgcca tcttgatggt caccgtgccc gacaccacca tgagatccga atggcggggc
     1861 gatgcccagg gcaccatgcc gagccgctcg acgtcgaacc gcgaagcgaa ggtcgccatc
     1921 atctcgatgg cgcagcacgc cagcccgaag gtcaccggcc agaccgacga ctttcgcgcc
     1981 cagttgaaga aggcatcggc gaccgtcgtc accacgaagc cgccggggaa gctgtggatg
     2041 ccttcggtga ccgattcgaa gagcgcggag tgtttgtcgc cctccggccg ctgacaccgg
     2101 agccgctccc actcctgaag gtccttgatg tcgcgccaga tcggcgcggg gatgacgggg
     2161 acgggcgcct ttatttccac tcgaggatgc cctcccggta cgcgtagatc catcccagaa
     2221 ggaggatcga caggaacatg agcatcgcaa gaatacccca ggctcccagt ccgcgaatgg
     2281 ccagcgccca gggaaacaga aagacggcca gcgcgtcgaa cacgatgaag acgagggcga
     2341 ccaggtagaa gccgaccgga aactgcaccc aggcctgtcc aatcggctcg gcgccgcact
     2401 cgtagctctg aagcttggcg gcaaagggac gtcccgggct gaggacgcgg gccgccccga
     2461 gcgagacgac cgcgaacgcc acgatgatgc cgagatagat gacgacgggc agatagtcgg
     2521 tcacgtgtgt acgtagtgtc cggcttcagc cggacctggc gcccgtagtc tacctgaagc
     2581 tctccatgat ctcgtcgagc gccgccttgc ccggccgcgt ccgcgattca tccaggtgcg
     2641 ccagcggctc gtcaaccagc ccgagcaggt cgatgaagtc cggcttgtcg ggctccacgt
     2701 agagaatccc ggtcgcgaac tcgttccgca tcgacgtctc gtgcagcagg cgcaacgcgc
     2761 gcagcttgtc ggtgggattg tagtcctccg tcatcttgct gagcacgagc ttggagccgt
     2821 cgtgcaacgt cacggtggtt gtcgtgccgg gatcgtagtc gacggagatc tcctcgaaga
     2881 acggcacgaa gctgacgtcg gcgagcggct cctcgtgatc cttggcgtag gagtagctct
     2941 tggtcgagcc gtcgtgatcg ttgaaggtga cgcagggcga cagcacgtcg atcatggcgg
     3001 tcccgcggtg gctgagcgcc gccttcagga tggccttcag ctgcttcgga tcgccggaga
     3061 acgagcgggc cacgaacgac gcccccagct cgatggccag caggcaggtg tcgatcggcg
     3121 gcaggtcgtt gatgacgccg gtcttcagct tcgatcccag gtcggcggtg ggcgagaact
     3181 gccccttggt gagcccgtag cagccgttgt cctcgatgat gtagaccatc ggcaggttgc
     3241 ggcgcatcag gtgcacgaac tgcccgatgc cgatggcccc cgtgtcgccg tcgccgctga
     3301 cgccgatggc gatcatcttc cggttggcga cgacggcgcc cgtcgcgaca gacggcatgc
     3361 ggccatggac ggagttgaag ccgtgcgact ggctcaggaa atacgcgggg ctcttgctcg
     3421 agcagccgat gcccgagagc ttgagaacct gggtcgggtc gacgccgagc tcgtagaacg
     3481 cgtcgatgat gcgctcggtg atcgcgttat ggccgcagcc ggcgcagagc gtcgtcttgc
     3541 tgccgcgata ggccgtcggc tcgagcccga tccgattgat cttcttccct ggtgccggct
     3601 gtgctggtgt cgacattctt ttatcccctg cccccttgcc cctgccccct gccccctgcc
     3661 ccctgccccc tgtcccctgt tgatcctcag tccgctccga ccgtcccgag tcccgagccc
     3721 ggcgtttcga gcccctcttt gccgtcctgc gtcaacaagt ccgtcgtcac cgaccggccg
     3781 tcgatcggca ggcccgcgta gtgccggatg ctccgcagct tgatcacgcg cgcgggcgtg
     3841 aggtcgatcc tgagcagcga catcatctgc gcgtcgcggt tctgctcgac gacgtagacg
     3901 tgatcgcacc ggtcgatgaa gtcgtcgagc tccttgctga acgggtaggc gcgcaggcgc
     3961 agatacgacg tcgtgacgcc atactcgccc ttcatctggt cgcgcgcttc aaccaccgcc
     4021 cagtgcgacg tgccgtacgc gatgatgccg atgcccccct cctcgaccag ctccacttcg
     4081 ggcctgggca tgatcgtctt ggccgtctgg aacttccgcg acagccgatc gagattcctc
     4141 atgtagtcgt cggcccgctc gctgtagagc gcctgctcgt tgtgacccga cccgcgcgcg
     4201 aagtacggcg gcagcccgtc accgggaacg gtgcgatacg gaatgccgtc gccgtccacg
     4261 tccttgtagc ggccgaactg tccgatgcgg gccagggccg ccttgtccag ccgcttgccg
     4321 cgattgaacg gcctggtcgg gtactcgaac ggctcggcca tccacgtgtt catgccgagg
     4381 tcgagatcgg acatcacgaa gatcggctgc tggagctgct cggccaggtc gaacgcctcc
     4441 atcgccatcg tgtagcactc ggcgaccgag gcgggaagca gcagcggatg gttcgtgtcg
     4501 ccgtgcgaca gcacggcggt ggagaggatg tcctgctgtc cggtgcgtgt cggcaatccg
     4561 gtggacggcc cggctcgctg gatgtcgaag atcacggccg gcacctcggc gtagtagcca
     4621 aggccggcga actcggccat cagcgacacg ccgggccccg ccgtggaggt cattgcccgc
     4681 gcgcccgtcc atccggcgcc gatcaccatc ccgagggccg ccagttcgtc ctcggcctgc
     4741 acgacggcat aggtcgtctt gccggtgtcg gggtcccgcc ggtacttctt caggtagtcg
     4801 atgagcgcct cgggaagcga cgaggacggc gtgatgggat accacgcgac gaaggtgacc
     4861 ccggccatca tgcagccgat ggccgcggcg gcgttcccct cgatcaggat catccccgtc
     4921 gtcttgttca tcggctcgat gacgaacggg tcctgcttgg tgaaggtcgc ctgggcgtag
     4981 tcggagcccg ccttgagcgc cgccatgttc atctcgagcg ccttctgctt gcgcccgagt
     5041 tgcttgcgca gcgccttctc catctgcccc atgtcgatgg cgagcaggcg cgagagcacg
     5101 ccatcgtaga tcatgttgcg gacgaggcgg cggagcttgg cgtcgtgggt gatcgtcccg
     5161 atgatcttgt cgaaggggac cgggtagaag accaggtcgc cgcgcagatc gcggagcttg
     5221 agcggctcgt cgtagacgac ggcggctccc ggcgcgagcg tcagcacgtc ctcacgcgcc
     5281 gtctccgggt tcagcgccac gaggaagtcc acttccttct tccggccgat gtagcccctg
     5341 gcgctggcgc ggatggtgta ccacgtgggc agccccgcga tgttcgaggg gaacatgttc
     5401 ttgcccgaca cgggcacgcc catctggaag atcgaccgga gcagcaccag gttcgcggtc
     5461 tggctgcctg atccgttgac ggtggcgact tggatggcga aatcgttgac gatgcgtgcg
     5521 gccggttcgc gtgtggcggc ctcgtgtacg gccgcgtcgg tcactgacat tggttctccc
     5581 gtacccccgg ggcccgattg ctcaaaagcg cgtggaaagc tgaggagaat tgcgcgcggg
     5641 gatcacggaa gtatatcacc cggctccgtg ggccccaggc gtcgggcccc ggggccctgg
     5701 cgaaaacgcc gtgctatcga accgtccgga gcatgcgtcc cagcgtcaag aacccaagcc
     5761 cgactgccag cccgattgcc aggcacgcca tccacagcca gggggcgccg gcgcgatcga
     5821 ggacgtagcc gccggcgatt ggcgccagcg cgaacccgct ggtaaacgcc atggcgaacg
     5881 cgccttgata ccgccctcgc agatgcgccg gcgccaggtc cgcgatgagc gacgtggagg
     5941 caggcgagaa aagaatctcg cccaacgtcc agacgacgac cgacgccgca tacgccggcc
     6001 aggatccgat ccacgcgttc atcccgaatc cgatcccgag cagcagcgac gccgcggcca
     6061 gcaccgacga gcgagggcga tcgcgaatca gctcgcccgc gagcggctga aagaggacga
     6121 tcaacacgcc gttgagcgcc atcagggcgc caaatccgga tggcgaaatg ccgtgcgcgc
     6181 gaacgtcgat tggcagagcg gcgaaggctt gaaagaagac gatgcagaag gccagcgtga
     6241 gcagacacac cgcgagaaag gcgtggtcct tgagaatcga cctcgccccg cttccgctgg
     6301 cgtcgctgtc tgccgagtcg cccggccgcg tctccgggag cgccgcccag acaatcaggc
     6361 cgaagagcgc ggtggtgacc gcatcggccg cgaagagcgc gcggtagctg cgggcggaaa
     6421 tgagaccgcc cacgagcgga gcgacagacg cgccgagatt caccgcccag tagatcaagc
     6481 tgtaggcgcg gggccgatcg tccggccgta cgatatcggc gatcgtcgcc gacacgacgg
     6541 gacgatacat ctcgtagagc aggcccgtca ccgctgacgc gatgatcatc gtcacgtacc
     6601 cggtgaccag gccgagcacg agcatgctcg ccccgccagc catgagcgac gcgaccagcg
     6661 tgggccgccg cccgaagcga tccgacagca cgcctgcgat gggaccgccg ccgatcgcgc
     6721 ccaggccgta cgccgtcagg atcgtgcccg cctggaccgg cgtcaggccg cgcgcgtccg
     6781 tgagatagat cgccaggaag acgacgacga atccaccgac gcggttgacg agcgtgccgg
     6841 cgaacagcac ccagaacggc cgcggcaggc cgcgtaggtc cggaaccagg cgggcacgct
     6901 gctgccgtac aatgcccacg tcctcgatca tgaagacatc cgcgctgttg gtccaggtgg
     6961 tgctggtggc ctctgtgacg gccacgccgc tgtcagcgca gtggatcaac gttccaccat
     7021 cggcctctcc acgcaccgcc gacgggaagc ccgatctcgc agcgcctgcg ccgcgattgc
     7081 ccgatggccg gcccgatctc tccggcgtct gggaaccgat cgacaaccgg ttcgtgggca
     7141 acatcgccgc gggcatcgga tccgagaacg taccgttcca gccgtgggcg aaggcgttat
     7201 tcgaatcgcg ccgcgacggg tcggcggcgc gcgacgatcc gcccgcgagc tgcctgccgc
     7261 aaggggttcc gaggctcggc gccgcgccgg cgccgtggag ggtcgtccag acaccgggac
     7321 tcgtcgtcat cgtgtatgaa gcgttcaatc tgtggcggca ggtctttctc gacggccgcc
     7381 aggtagcggc cgacgccaat ccgacctggc tgggatattc gaccggcagg tgggagggcg
     7441 acgcgctcgt tgtcgaaacg agaggcttca acggcaagac gtggctggat cagatggggc
     7501 ggccgacgac cgaagcgct
//
LOCUS       DBNGSH010000340         2307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3763599, whole genome shotgun sequence.
ACCESSION   DBNGSH010000340 DBNGSH010000000
VERSION     DBNGSH010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3763599"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="AC16CY_08000"
     CDS             <1..884
                     /locus_tag="AC16CY_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3353767.1"
                     /translation="PARVVVIMMILVTSKLTLVLAATVVAFAAPMAQAPAPSQAAPAF
                     EVASIRKNVSVSDGASVRAQPGGRLTVSNNTLRNIIRNAYNVQNYQIIGGPDWMSSDR
                     WDITAKAADDTAPPEMLLMLRTLLADRFKLVIRRETRQLPTFAVVLARPDGRLGPQLR
                     VSTVDCAALFAAAKARGQQPPPTTNGRPTCGTQTQRGTMMTTGTSMADFARNIAPSAG
                     RPVVDKTGLTGAYDIDLTWTPDQGPPNPDGAAAPPAGDGVSLFTAVQEQLGLKLDAQL
                     GPVDVLVIDSVQPPTED"
     gene            1076..1624
                     /locus_tag="AC16CY_08005"
     CDS             1076..1624
                     /locus_tag="AC16CY_08005"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3353768.1"
                     /translation="MSEATRLVTAEELARFPDDGYRYELVEGRVIRMSPVSFDHGRIV
                     VQLATLLHRLVQQQPVGVVVMDVGFTLASNPDTVRGPDIAFVRRDRVPPKGARGFFKG
                     PPDVAIEVLSPDDRPGEVRAKTDEYPARGVRLVVVIDPDEKTATTIRPNGPHVILREE
                     DDLLDLNEVIPGFSCLLREIFE"
     gene            1634..>2307
                     /locus_tag="AC16CY_08010"
     CDS             1634..>2307
                     /locus_tag="AC16CY_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR3353769.1"
                     /translation="MAARFPDWVVPMAATLTEKRFEGPEWTFERKLDGIRLLAFKQGR
                     DVGLFSRNRLPQHSPQITDAIAALPARDLILDGEVTGVWGPASTRAADRPRGLAYHVF
                     DILWLDGRDLMSLPLDQRRERLRALPLRAPLERVAALDVPKPWEHACRKGWEGVIAKR
                     RDSMYEHRRSPHWLKMKCEASQELVVGGFTDPQGARVGLGALLVGYFDAEDFVFAGKV
                     GTGLDT"
BASE COUNT          397 a          775 c          767 g          368 t
ORIGIN      
        1 gcccggcgcg cgttgttgtc atcatgatga tcctcgtgac atcgaaactc accctcgttc
       61 tcgcagccac tgtcgttgcg ttcgccgcac cgatggcgca agcgcccgct cccagtcagg
      121 cagcaccggc gttcgaggtc gcgtcgatcc ggaagaacgt gtcggtcagc gacggcgcgt
      181 cggtccgcgc ccagcccggg ggccggctga cggtgagcaa caacacgctc cgcaacatca
      241 tccggaacgc ctacaacgtt cagaactacc agatcatcgg cgggccggac tggatgagca
      301 gcgatcgctg ggacatcacg gcaaaggcgg ctgacgatac ggcgccaccg gagatgctgc
      361 tcatgctgcg cacgctgctc gcggatcggt tcaagctcgt cattcgacgc gaaactcgcc
      421 agctgcccac gttcgcagtc gtgctcgctc ggcctgatgg gcgcctcggg ccgcagcttc
      481 gcgtctcgac agtggactgc gcggcgctct ttgctgctgc caaggcgcga ggccagcagc
      541 cgccgccaac gaccaacggc cgccccacct gcggcacgca gacgcaaagg ggaaccatga
      601 tgacgaccgg cacgtcgatg gcggacttcg cgcggaacat cgcgccttct gcgggacgtc
      661 ctgtcgtgga caagacgggg ctgacgggag cctacgacat cgacctgacg tggacgcccg
      721 atcagggacc gccgaatccg gatggagcgg ccgcgccgcc ggcaggcgac ggcgtctcgc
      781 tcttcaccgc ggtgcaggag cagctcgggc tcaagctgga cgcacagctc ggtccggttg
      841 acgtcctcgt gatcgactcg gtgcagccgc cgacagagga ctgatcacgg gtccggctga
      901 agccggacac ttcgtacgta accgatcacg ggtcgcaaga ccgaccgacg tactggtccg
      961 cgcgtgagcg tcgcaatagc tgcaccgttt ccgatcccgc accgcgattc cttcgagatt
     1021 ccgcgcggca tggacttggc gatgacgatg ctaagatgcc atgcgaagca accgtatgtc
     1081 cgaggcaacg cgtctggtca ccgccgagga actggcgcgg ttcccggacg acggttaccg
     1141 gtacgaactg gtcgaaggcc gggtcatccg catgagtccg gtcagcttcg accacggcag
     1201 gatcgtggtg cagctggcta cgctcctgca tcgcctcgtc caacagcagc ccgttggggt
     1261 cgtcgtgatg gacgtcggtt tcacgctcgc ctcgaacccc gacacggtca gagggcccga
     1321 catcgcgttc gtacgacggg accgagtgcc gccgaagggt gcgcgcgggt tcttcaaagg
     1381 tccacccgac gtcgcgatcg aggtgctgtc gccggacgat cggccgggcg aggtgcgagc
     1441 gaaaacagac gagtacccgg cacgcggcgt acgtctggtc gtggtgatag accccgatga
     1501 gaagaccgcg acgaccatcc ggccgaacgg gccccacgtc atcctgcgag aggaagacga
     1561 cctcctcgac ctgaacgaag tgatccccgg cttctcctgt ctcctccgag agatcttcga
     1621 ataaccttcc ctcgtggccg cgaggtttcc cgactgggtc gtgccgatgg cggcgactct
     1681 cacggagaag cggttcgagg ggccggaatg gaccttcgag cgcaagctcg acggcatccg
     1741 cctgctcgcc ttcaagcagg gccgcgacgt ggggctgttc tctcgcaacc ggcttccgca
     1801 gcacagtccg cagatcaccg atgcaatcgc ggcgcttccg gcgcgtgacc tgatcctcga
     1861 cggcgaagtc accggcgtgt ggggaccggc gagcacgcgc gccgcggacc ggcctcgcgg
     1921 gctcgcctat cacgtcttcg acatcctgtg gctcgatggg cgcgacctga tgtcgctgcc
     1981 gctcgaccag cgccgcgaac ggctgcgcgc gctccccctt cgcgcgccgc tggagcgcgt
     2041 ggccgcgctc gacgttccga aaccgtggga gcacgcctgc cgcaaggggt gggagggcgt
     2101 catcgccaag cggcgcgatt cgatgtacga gcaccgccgc tcgccgcact ggctcaagat
     2161 gaagtgcgag gcgagccagg agctcgtcgt cggcgggttc acagacccgc aaggcgcgcg
     2221 agtggggctg ggggctttgc tcgtcggcta cttcgatgcc gaggatttcg tcttcgccgg
     2281 gaaagtcggc acgggcctcg acacgaa
//
LOCUS       DBNGSH010000341         8184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_367374, whole genome shotgun sequence.
ACCESSION   DBNGSH010000341 DBNGSH010000000
VERSION     DBNGSH010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_367374"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="AC16CY_08015"
     CDS             complement(<1..485)
                     /locus_tag="AC16CY_08015"
                     /inference="COORDINATES: protein motif:HMM:NF018743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HIR3353770.1"
                     /translation="MLRLGSDVLLASGRLDGARVGIVCNHASVDGRFAHVVDRLASAP
                     GVTLAAIFGPQHGFRSDVQDNMIETAHADDPGRRVPVYSLYSETREPTADMLKGVDVL
                     VVDLQDVGARIYTFIYTMANCLRACARHGVPVIVCDRPNPIGGLDVEGALLSPGYESF
                     VG"
     gene            complement(521..1843)
                     /locus_tag="AC16CY_08020"
     CDS             complement(521..1843)
                     /locus_tag="AC16CY_08020"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 8 glycosyl hydrolase"
                     /protein_id="HIR3353771.1"
                     /translation="MPNRISRVIRAREILPVRLHVLLPVSVALALTFVGGRASANPTD
                     PFLWPYNQTTNITFDAAAIAQAWQEWKSAQITSTNAGGEGRLRVMGGIDASSTVSEGQ
                     GYGILFASIFDDQATLDGLWLFTADHLNAHGLMDWHIGNPGQRIGPGAATDADEDIAL
                     GLLNACVKVRRGSWPASPRGIDYCARAAAMIDAIYQYEIDKAGGGHPVGLPDNPGNEL
                     LPGDAWTLTDYPRGIVNLSYFSPGYLTVFGKFTNRQAEWQAVNARNYALVNLAQDRPG
                     NCSKLVPNWTRYDGEPQKVSWQPDNYSWWSYDAARLAWRVAVDRRWYGSAESRAIMNE
                     MGSFFSSAGLGSIGEHSMNGRRTGGGPSPFFVANAGSAIWAAERLTAVTCGAATGRLK
                     ETPQSAFNRVLDTKDTPTSYYGNAWRILAMLLMTGNFPNFYEMAAERR"
     gene            complement(1868..2251)
                     /locus_tag="AC16CY_08025"
     CDS             complement(1868..2251)
                     /locus_tag="AC16CY_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353772.1"
                     /translation="MVALVALAFAFTAYGQAASPAPQGQSPAAQAPAAPAQQAPGQAA
                     VATAQGELLEVDAKASTFAIKTAAAEMKFRYDDSTKVSGAQKGVAGLATMTGSQVTVQ
                     YKKEGANNIATSIEVRGGQGPAPGR"
     gene            2727..3287
                     /locus_tag="AC16CY_08030"
     CDS             2727..3287
                     /locus_tag="AC16CY_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HIR3353773.1"
                     /translation="MQPRRDARSLSLTEPSSTVARSAKVEKRFGRLVWVLLLLIPRYA
                     IAADPPAGTATAPTPRRDVNFIQSLVDDLRGRLTLPQHVRVSIVPTNPLKVSVQSVDE
                     LGGEFLLSFEESFLSELTDDELKAAIAHELGHVWIFTHHPYLQTEALANQIAARVVTR
                     DSLVQVYEKVWKLHGTKNDLARLVSD"
     gene            3514..7974
                     /gene="bcsA"
                     /locus_tag="AC16CY_08035"
     CDS             3514..7974
                     /gene="bcsA"
                     /locus_tag="AC16CY_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016759 - cellulose synthase activity
                     [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HIR3353774.1"
                     /translation="MLTDDPRSRHVDVGELILDRGVAAPVSRPRRTQARGRHPSRRSF
                     GRRLLSAIRTLLALASTCALGVLLVAPLGWKEQAIAGGALIAAAILLSGLSRSRTVTL
                     ALMAVSIFSTLRYAYWRTGETWNGITSAGHLYQWDTVFVLLLLAAEFYAFATLALGYF
                     QTLHPLRRPPVPLRGDPHAWPTVDVFIPTYNEPLDVVRATVLGALELDYPADKINIFV
                     LDDGRRREFKDWTAHVGVGYITRGDNAHAKAGNINHALTHTRGELVAIFDCDHVPTRS
                     FLQTTLGWFARDEMLGLVQTPHHFYSPDPFERNLAQFRKVPNEGALFHRLVQDGNDLW
                     NASFFCGSCAVIRRVALEEIGGIAVETVTEDAHTALRMQRRGWNTAYINIPQAAGLAT
                     ESLSAHIGQRIRWARGMVQILRIENPLLASGLSFTQRLCYFNATTHYLFAVPRLVFLT
                     IPLTYLLFGMVNIYGYSLAVFAYALPHIVLSQLANARVQKGYRSSLWNEVYEAVLAPY
                     ILLPTLLALINPRLGKFNVTAKGGVIERSYFDHRVALPFLLLLGLNIAGLVMAYQRYV
                     TDPAHHDTVIMNAVWTVYNIVILSVAASVARERRQRRTDVRVDVRLPLTLVTPEGEHV
                     AGVSAQLSRGGAAACFGRRVRLSRHAPVTVVLHARGAHCEIAARVVSWRRGRQVVHLL
                     FEQLNTAQERFVVNAIYSRPAAWVESDAPGRTDSPLKSIGRILWLSLRGIVVVLFGLL
                     TPPRRGSDTHSTKNRRRAAAAAASMLAIAVASAGPARAAQRPDEAAAVTPSMFRDAYE
                     LGEIAGQRATALQGPGASLNFYFGVPVTKIISEASLVLRYAAPLLEPGDGRLELLLNG
                     SRIGSVALTPGTGVEVDVPLPTDLLTTDNTLSLRLEGQCVACAKASAPWITIDPASRL
                     RIGGTRLPLANDLSLLPLPFFDPSSRRAWRLPVVFSAPLELDEIRAAAVVASWFGVAS
                     DVRGVRFPVTIDALPEGHALVIARRGSALASMLSLPPAPATLVAIRDNPRDPYGKLLI
                     VVGDRPAELLEAALALVTLDLSRLQADVLPGQDVRVPGRGAYAAPRWLENARSAPIGL
                     YTTADRLKLVGSGSIDVFFRLPPDLFLPIRQSIPLVLRFAYAGVPEGANAALHVRLNA
                     EDVDTIRLEPSLGPVQREEIVRLPTGRLRPYANALTVDIDFGRDGAPSGVTQYAAIDR
                     ESALDLRGIPHWVALPKLELFVDAGYPFTAWPDLSRTAIVLSNAPTPIEYEVLLNMVA
                     FFSAQTGSPATAISIANAAEVESVRDKDLVLLGAPSTQPWLGEWVRQMPLGLSIDGPL
                     LHDDGPLLDRVRRPGFWLRPQWPFRNEGRSRLSRLIGRGEPLDVVLEQFVSPFRPDRS
                     VVAIVHGERGGEMVASLFAGIRSGPIYGGLAVAHDGRFEAFPLGIQPYHAGEVDPYQR
                     AQVFLVEYYLFIPPFVLLVALLIGSGVRDATERVSARRLMPGRTWEGWST"
     gene            7971..>8184
                     /locus_tag="AC16CY_08040"
     CDS             7971..>8184
                     /locus_tag="AC16CY_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353775.1"
                     /translation="MTRCARGLGVVLLAFLVPALAAQTRDGVDVLLGKARSLEARGRM
                     DLAAQNWSQVLLVDPDQREALAGLARH"
BASE COUNT         1235 a         2825 c         2688 g         1436 t
ORIGIN      
        1 cccacgaagg actcgtagcc cggcgagagc agcgcccctt ccacgtcgag gccgccaatc
       61 ggattcggcc ggtcgcacac gatgaccggg acgccgtggc gcgcgcacgc gcgaaggcag
      121 ttcgccatcg tgtagatgaa ggtatagatc cgcgcgccga cgtcctgcag atcgacgacg
      181 agcacgtcca cgcctttcag catgtccgcc gtcggctcac gcgtttcgct gtagagcgag
      241 tagaccggca cgcgccgccc ggggtcgtcg gcgtgggcgg tctcgatcat gttgtcctgg
      301 acgtccgacc tgaacccgtg ctgcggcccg aagatcgcgg cgagcgtgac gccgggcgcc
      361 gacgcgagcc gatcgacgac gtgcgcgaag cggccgtcga ccgaggcgtg gttacagacg
      421 atcccgacgc gggcgccgtc gaggcggccg gacgccagca gcacatcgga gccgagacga
      481 agcatgaggt tccattatct agcgaggccg cgcctcgcga ctatcgtcgc tcggccgcca
      541 tctcgtagaa gtttggaaag ttgcccgtca tcagcagcat ggccaggatc cgccaggcgt
      601 tgccgtagta gctcgtcggc gtgtctttgg tgtcgaggac gcggttgaac gccgattgag
      661 gcgtttcctt gaggcggccc gtcgccgcgc cgcaggtcac agcggtcaaa cgctcggcgg
      721 cccagatggc cgatcccgcg ttggcgacga agaacggcga gggtcctccg cccgtgcgcc
      781 gtccgttcat gctgtgctcg ccgatgctgc cgagaccggc gctgctgaag aagctgccca
      841 tctcgttcat gatcgcgcgg gactcggccg aaccgtacca tcgtctgtcc acggccacac
      901 gccacgcgag ccgcgcggca tcgtagctcc accacgagta gttgtccggc tgccaggaca
      961 ccttctgcgg ctcgccgtcg taccgtgtcc agttcggcac cagcttggaa cagttgcccg
     1021 gcctgtcctg ggccaggttc acgagcgcgt agttgcgcgc gttcaccgct tgccattcgg
     1081 cctgacggtt ggtgaacttg ccgaagacgg tgaggtagcc gggcgagaag tacgagagat
     1141 tgacaatgcc tcgtgggtag tcggtgaggg tccaggcgtc gccgggaagc agctcgttgc
     1201 ccgggttgtc gggcagaccc accggatggc cgccgccggc cttgtcgatc tcgtactggt
     1261 agatggcgtc gatcatggcg gcggcacgcg cgcagtagtc gatcccgcgc ggactggcgg
     1321 gccacgatcc tcgccgcacc ttgacgcagg cgttcagcaa gccgagggcg atgtcctcat
     1381 cggcgtcggt ggccgcgccc gggccgatgc gctggcccgg attgccgatg tgccagtcca
     1441 tcagcccgtg cgcattcagg tggtcggccg tgaacagcca caggccgtcc agcgtcgcct
     1501 ggtcgtcgaa gatggacgcg aagaggatgc cgtagccctg gccctcggat accgtggagg
     1561 aggcgtcgat cccgcccatg acgcgcaggc ggccctcacc gcccgcgttc gtgctcgtaa
     1621 tctgcgcaga cttccactcc tgccacgcct gggcgatggc ggcggcatcg aacgtgatgt
     1681 tggttgtctg gttgtacggc cacaggaagg gatccgtggg attggcggat gcgcgccctc
     1741 cgacgaacgt caaggcgagc gccacggaca ccggcaacag cacatgcagc cggaccggca
     1801 ggatttcacg agcccgaatg actcgtgaaa tcctgttcgg cacggcgttc agcctcgctc
     1861 gaccccgtca tcgaccgggc gctggtccct gacccccgcg cacttcgatg ctggtggcga
     1921 tgttgttcgc tccttctttc ttgtactgca ccgtcacctg ggaacccgtc atcgtcgcca
     1981 ggccggcgac tcccttctgg gccccggaga ccttggtcga atcgtcgtag cggaatttca
     2041 tctcggccgc cgcggtcttg atggcgaagg tgctggcttt cgcatcgact tccagcaact
     2101 ccccctgggc cgtcgccacg gctgcctggc ctggtgcctg ctgagccgga gcggcgggcg
     2161 cctgagccgc cggagactga ccctgcggag ccggagaagc cgcctggccg tacgcggtga
     2221 acgcgaacgc gagcgcgacc agagcgacca atgttcgtag actcagttgc ataaaggaac
     2281 ctcccgcgca gacacttggc aaccgccgta ccattccctc acgcgccgga atgtgcgtcc
     2341 ttcgttcgag tgaacgagaa acgtgcggct gagatcggtg cgagtcaggg agtagtcctt
     2401 acgcagccat gtagttcgtg cctcctgcga tctcagcagc gaactcatcc acgcggcggc
     2461 tgaagccgcc gcgctacggt gccggctgaa gccgccgcgc tacggtgccg gcacaacgcg
     2521 catgtcatct gctcagtccc cggcgccgtg ctatctttta gtcatgcgtc gccgctcccg
     2581 cggcgcatcc tgccgggcac tccacaaatc agcggcgcgg cgatttggat cgtgtcgatg
     2641 gagcgagcgg aattcgtgtg gctggcagcg tctaccggga gacctgtgtt tcgccggggc
     2701 gaaccatggg aggggccggg tgacacatgc agcctcgacg agacgcgcgt tccctttctc
     2761 taacagagcc ctcgtccacc gtagcgcgca gcgcgaaggt ggagaagcgg ttcggccgtc
     2821 tcgtgtgggt gctgttgctg ctcattcctc gctacgcgat cgcggctgat ccgcccgctg
     2881 gcacggcgac cgcgccgaca ccgcgtcggg acgtgaactt cattcaaagt ctggtcgatg
     2941 atctccgcgg ccgactcacc ctgcctcaac acgtgcgtgt ctcgatcgtg ccaaccaacc
     3001 cgctcaaggt gtcggtgcag tcggtggacg aactcggcgg cgagtttctg ctgtcgttcg
     3061 aggagagctt tctgtcggag ttgaccgacg acgaattgaa ggccgctatc gcgcacgagc
     3121 tcggtcacgt ctggatcttc acgcatcatc cctatctgca gaccgaagcg ctcgccaacc
     3181 agatcgccgc acgcgtcgtg acgcgcgaca gcctcgtgca ggtgtacgag aaggtgtgga
     3241 agctgcacgg caccaagaac gatctcgcgc gtctcgtcag cgattgacgc gcgcccgcgg
     3301 gcatcgcccc aaagacgacc agaagagctg gtgacctgtc cagctcgtca cgagccgact
     3361 gagtaattct gtcagcctcg gcacgcccct cctctccggt tcaggtcacc tcgcattcgc
     3421 cggttgcagt tggaacgcaa cgagttaccc aaatcgccaa ctcgacctgg tcttgcgagc
     3481 atcgatattg ctaaatggcg tccccgtgga acgatgctga cggacgaccc ccgctcgcgg
     3541 cacgtggacg ttggtgagct catcctggat cgcggcgtcg ccgcgccagt gtcacgcccg
     3601 cggcgcacgc aggcgcgcgg gcgccatccg tcgcggagat cgttcggacg ccgtctcctg
     3661 tctgcgatac gcacgctgct cgccctggcc agcacgtgcg cgctcggcgt gctgctcgtg
     3721 gctcctctgg ggtggaagga acaggcgatt gcgggcggcg cgctgatcgc cgccgcgatt
     3781 ctgctctcgg gcctctcgcg ctctcgcacc gtcacgctcg cgctgatggc ggtctcgatc
     3841 ttcagcacgc ttcggtacgc gtactggcgc acgggcgaga cctggaacgg catcacgagc
     3901 gcgggccacc tgtatcagtg ggacacggtg ttcgtgctgc tcctgctggc cgccgagttc
     3961 tacgcgtttg cgacgctggc gctcgggtat ttccagacgc tgcacccgct gcggcggccg
     4021 cccgtgccgc tccggggcga tccgcacgcc tggccgaccg tcgacgtctt cattccgacc
     4081 tacaacgagc cgctcgacgt cgtacgggcg accgttctcg gcgcgctcga gctcgactac
     4141 ccggccgaca agatcaacat cttcgtcctc gacgacggcc ggcgcaggga gttcaaggac
     4201 tggaccgccc acgtcggtgt cgggtacatc acgcgtgggg acaacgccca cgccaaggca
     4261 ggcaacatca accacgcgct cacacacacg cgcggcgagc tggtggccat cttcgattgc
     4321 gatcacgtgc cgacgcggtc gttcctgcag accacgctcg gctggttcgc gcgcgacgag
     4381 atgctcgggc tggtccagac gccgcatcac ttttattccc cagatccgtt cgagcgcaat
     4441 ctcgcccagt tccgcaaggt ccccaacgag ggcgcgctgt tccaccgcct cgtgcaggac
     4501 ggcaacgacc tctggaacgc gagcttcttc tgcggctcgt gcgcggtgat caggcgcgtg
     4561 gcgctcgaag agatcggcgg catcgccgtg gaaacggtga ccgaggacgc ccacaccgcc
     4621 ttgcggatgc agcgccgcgg ctggaacacc gcctacatca acattccgca ggccgccggt
     4681 ctggccaccg agagcctgtc ggcgcacatc ggtcagcgca tccgctgggc gcgaggcatg
     4741 gtgcagattc tgcgcatcga gaacccgctc ctggcgagcg gactctcgtt cacgcagcgt
     4801 ctctgctact tcaacgcgac gacccactat ctctttgcgg tgccgcgcct cgtcttcctc
     4861 accattccgc tgacctatct gctgttcggg atggtgaaca tctacggcta ttcgctggcg
     4921 gtcttcgcct acgcgctgcc gcacatcgtc ctgtcgcagc tggccaacgc ccgcgtccag
     4981 aagggttacc gatcgtccct gtggaacgag gtctacgaag ccgtgctggc gccgtacatc
     5041 ctgctaccca cgctgctggc gctcatcaat ccacgtctcg gcaaattcaa cgtgacggcc
     5101 aagggcggcg tcatcgagcg gtcgtacttc gaccatcgcg tggcgctgcc gttcctgctg
     5161 ctgctcggcc tcaacatcgc cggcctcgtc atggcctacc agcgctacgt caccgatccc
     5221 gcgcaccacg acacggtgat catgaacgcc gtctggacgg tctacaacat cgtgatcctc
     5281 tcggtggccg cctccgtcgc acgcgaacgc cggcagcgcc gcactgacgt ccgcgtggac
     5341 gtgcggctgc cgttgacgct ggtgaccccg gaaggcgagc acgtggccgg cgtgtcggcg
     5401 cagttgtcgc gcggaggcgc cgcggcatgc ttcggcagaa gggtgaggct ctcacggcac
     5461 gcgccggtga ctgtcgtgct gcacgcacgc ggagcccact gcgagatcgc ggcgcgcgtg
     5521 gtgtcgtgga ggcgcggacg gcaggtggtg cacctgctgt tcgagcagct gaacacggca
     5581 caagagcgct tcgtcgtcaa cgcgatctac tcgcgcccgg cggcctgggt cgagtcggac
     5641 gcgcccggac gaaccgattc cccgttgaaa agcattggcc gcatcctctg gctgtcgctg
     5701 cgcggcatcg tggtggtgct tttcggcctg ctgacgccgc cgcgccgcgg cagtgacaca
     5761 cactcgacga agaaccgccg ccgcgccgcg gctgccgccg cgtccatgct ggcgatcgcc
     5821 gtggcgtcgg ccgggcccgc ccgggcagcc cagcgcccgg acgaggcggc ggccgtcacg
     5881 ccctccatgt tccgagacgc ctacgagctc ggcgagatcg ccgggcagcg ggccaccgcg
     5941 cttcaggggc cgggagcctc gctcaacttc tactttggcg ttccggtcac gaagatcatc
     6001 agcgaggcgt cgctcgttct ccgctacgcg gcgccgctcc tcgaacccgg cgacggccgg
     6061 ctcgagctgc tcctgaacgg ctcgcgcatc ggctccgtcg cgctcacgcc tggaacaggc
     6121 gtcgaggtgg acgtcccctt gccgacggat ctgctgacga ccgacaacac gctctcgctc
     6181 aggctggaag gccaatgcgt cgcgtgcgcg aaggcatcgg cgccatggat caccatcgac
     6241 cctgccagcc ggctccggat cggcggcacg aggctgccgc tggcaaacga tctgtcgctg
     6301 ctcccgctcc cgttcttcga cccctcgtcg cgccgcgcct ggcgtctgcc cgtggtcttc
     6361 tccgcgccgc tagaactcga cgagatcagg gccgcggccg tcgtggcgtc gtggttcggc
     6421 gtcgcctccg acgtccgggg cgtcaggttc ccggtgacga ttgacgcgct gccggagggt
     6481 cacgccctgg tgatcgcccg ccgcggctcc gcgctcgcgt cgatgctgtc gttgccgccg
     6541 gcccccgcga cgctcgtcgc gatccgcgac aacccgcggg atccctacgg caagctgctg
     6601 atcgtcgttg gcgaccggcc tgcggagctg ctcgaggccg cgctggcgct ggtcacgctc
     6661 gacctctcac gcctgcaggc ggatgtgctg cccggacagg acgtacgcgt gccaggcaga
     6721 ggagcgtacg cggcgccacg ctggctggag aacgcccgat cggcgcccat cggcctctac
     6781 accaccgccg atcgcctgaa gctggtcggg tccggctcga tcgacgtgtt cttccgtctg
     6841 ccgccggact tgtttctgcc catccgccag tcgattccgc tcgtcctccg gttcgcctac
     6901 gcgggcgtgc ccgagggcgc caacgccgcc ctgcacgtgc ggctgaacgc cgaggacgtg
     6961 gacaccattc gactcgagcc ttcgctcggg cccgtacagc gggaggagat cgtccgcctg
     7021 ccaacgggcc ggctgcggcc ctatgcgaac gcgctgacgg tggacatcga tttcgggcgt
     7081 gacggcgccc cgtcaggagt gacccagtac gccgcgatcg atcgcgagtc ggccctcgac
     7141 ctccgcggca ttcctcactg ggtggcgctt cccaagctcg agctcttcgt ggacgccggc
     7201 tatccgttca ccgcgtggcc cgatctgtcc cgcaccgcga tcgtgctctc gaacgccccc
     7261 acgcccatcg agtacgaggt cctgctgaac atggtggcgt tcttcagcgc gcaaaccgga
     7321 tcgccggcaa ctgcgatctc gattgccaac gccgctgaag tcgaatccgt gcgcgacaag
     7381 gacctcgtgc tgctgggtgc gccatcgacg caaccgtggc tgggggagtg ggtgcgccag
     7441 atgcctctcg ggttgtcgat cgacgggccg ctgttgcacg acgacgggcc cctgctcgac
     7501 cgcgtccgcc gacccggttt ctggctgcgc cctcagtggc cgtttcgcaa tgaaggccgc
     7561 agcaggctgt cgaggctgat cggacgaggg gagccgctcg acgtcgtcct cgaacagttc
     7621 gtgtcgccgt ttcgcccgga tcgatcggtc gtggcgatcg tgcacggcga gcgcggcggc
     7681 gagatggtcg cgtcgctctt cgcggggatc cggagcgggc ccatctacgg cggcctcgcc
     7741 gtcgcccatg acgggcgatt cgaggccttt ccgctgggta tccagccgta tcacgcgggc
     7801 gaggtcgatc cgtatcagcg cgcccaggtg tttctcgtcg agtactacct gttcattccg
     7861 ccgttcgttc tgctggtcgc tctgctgatc ggcagcgggg tgcgcgacgc aaccgaacgc
     7921 gtcagcgcac gccggctgat gccggggcga acctgggaag gctggtccac atgactcgat
     7981 gcgcacgcgg tctcggagtg gttctgctcg cgttcctcgt accggcactg gccgcgcaga
     8041 cgagggatgg ggtcgatgtg ctgctcggca aggcgcggtc gctcgaggcg cgggggcgga
     8101 tggacctcgc cgcacagaac tggagccagg tccttctcgt cgatcccgac cagcgagagg
     8161 cgcttgcggg cctcgcgcgc cacg
//
LOCUS       DBNGSH010000342         2196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_537675, whole genome shotgun sequence.
ACCESSION   DBNGSH010000342 DBNGSH010000000
VERSION     DBNGSH010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_537675"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="AC16CY_08045"
     CDS             complement(<1..371)
                     /locus_tag="AC16CY_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3353776.1"
                     /translation="MALTRNQKRGFLAAWGGWALDGMDASIYALVLVPALMDLLPRSG
                     VETSQGNIGYYGSILQAMFLLGWGLSMVWGPISDRVGRVRALMLTILCYSLFTFMCGL
                     VNNIWQLAVLRVLVGIGIGGE"
     gene            complement(375..1679)
                     /locus_tag="AC16CY_08050"
     CDS             complement(375..1679)
                     /locus_tag="AC16CY_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3353777.1"
                     /translation="MRKNQAAAIFGVFVVAAAAMIVSWHARVEGQAQTTSIAAIPSEK
                     GGQDVFGGYDAAAWPKPLTSIKGHEGWTWGAGQYVFAESANRVFVLQRGELPAFPKRP
                     QTIRLPQLGPSIEFPMFRLPIRDATSASPPGAMFGPDGKTPGDDTDVGKPGLDWRWEH
                     IVTVFDAQGNLIEDWTQWDKMFRRPHAIYISPYDPQKNVWIVDDYRHAIFRFSNDGKK
                     LLQTIGEPNVPGNDDKHFNRPTFMAFLPDGTFFVADGYTNTRVVKFDKDGKYLMTWGQ
                     RGEAGRGASGPEKRPGYFNNVHGIAVDPQTRRVFVNDRNNGRVQVFDDNGKYLDEWSF
                     GPRPVSDVHMFIITSDRFLWAADRGTSKLLKYSLDGKFLYSWGVWGDFQGGFWGVHGM
                     SVDQDGNVYTAAVDSGGAQKFTPRKGANPAFLLGRQVNPGWK"
     gene            1798..>2196
                     /locus_tag="AC16CY_08055"
     CDS             1798..>2196
                     /locus_tag="AC16CY_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR3353778.1"
                     /translation="MRRSATLLAVVAAAGFLTSPVETQSAGDGGEWAEKARLGFERTE
                     AAVAVVAGRVYVVGGMSRGQDSDTLTQEYDPKTDRWRERAPMPRPLSHPGAAVLNGKI
                     YVVGGFLRNVHLDAQSDAFAYDPATDRWEML"
BASE COUNT          369 a          742 c          706 g          379 t
ORIGIN      
        1 tgttcaccgc cgatgccgat gccgacgagc acgcgcagga cggcaagctg ccagatgttg
       61 ttgacgagcc cgcacatgaa cgtgaacagc gaatagcaga ggatcgtcag catcagcgcg
      121 cgcacgcgcc cgacacgatc gctgatcggc ccccacacca tcgacaggcc ccagccgagc
      181 agaaacatcg cctgcaggat gctgccgtag tagccgatgt tgccctggga ggtttcgaca
      241 cccgagcgcg gcagcaggtc catcagcgcc ggcacgagga cgagcgcgta gatagacgcg
      301 tccatcccgt cgagcgccca gccgccccac gcggccagga acccgcgctt ctgattccga
      361 gtgagcgcca tccgctattt ccagcccgga ttgacttgcc tgccgagcag gaatgcgggg
      421 ttggcaccct tccgcggcgt gaacttctga gcgcctccgc tgtccaccgc cgcggtgtag
      481 acgttgccgt cctggtccac gctcatgccg tgtacgcccc agaagccgcc ctggaaatcg
      541 ccccacacgc cccaggagta caggaacttt ccatcgaggc tgtacttcag gagcttcgag
      601 gtgccccgat cggcggccca caggaaccgg tcgctcgtga tgatgaacat gtgaacgtcg
      661 gagaccggcc gcgggccgaa gctccattcg tcgaggtact tcccgttgtc gtcgaacacc
      721 tgcacgcggc cgttgttgcg gtcgttcacg aagacgcgcc gcgtttgcgg atccacggcg
      781 atgccgtgca cgttgttgaa gtagcccggg cgcttctccg gtccgctggc ccctctgccg
      841 gcttcaccgc gctggcccca ggtcatcaga tacttgccgt ccttgtcgaa cttcaccacg
      901 cgcgtgttcg tgtagccatc ggccacgaag aaggtgccgt cgggcaggaa cgccatgaac
      961 gtcggccgat tgaaatgctt gtcgtcgttg cccggaacgt tcggctcgcc gatcgtctga
     1021 aggagcttct tcccgtcgtt cgagaacctg aagatcgcgt ggcgatagtc gtccacgatc
     1081 cagacgttct tctgcgggtc gtaggggctg atgtagatgg cgtgcggacg ccggaacatc
     1141 ttgtcccact gggtccagtc ctcgatcagg ttgccctgcg cgtcgaagac ggtgacgatg
     1201 tgctcccagc gccagtcgag gccgggcttg ccgacgtccg tgtcgtcgcc gggcgtcttg
     1261 ccatcgggac cgaacatcgc tccgggcggg cttgccgacg tggcgtcgcg gatcggcagc
     1321 cggaacatcg ggaactcgat gctcggtccg agctgcggaa ggcggatcgt ctgcggccgc
     1381 ttcgggaacg cgggaagctc gcctcgctgg aggacgaaca cgcggttcgc gctctctgcg
     1441 aagacgtact gacccgctcc ccacgtccac ccttcgtgac ctttgatcga ggtcagcggc
     1501 ttgggccacg ccgcggcgtc atagccgccg aagacgtcct ggccgccctt ttccgacggg
     1561 atggcggcaa tgctcgtggt ctgggcctga ccttccaccc tcgcgtgcca cgacacgatc
     1621 atcgcggcgg ccgcgacgac aaacacgcca aagatggcgg cagcttgatt cttgcgcatg
     1681 ggctcccctc tccgctcaga gttgagcccc gtttctagag cccctgaccc cgaatgtcaa
     1741 gaacgatcgc cgcacccctt gacgtcatcg cgactgccgg ggaagatgcg cctcaccatg
     1801 aggcgttccg ccacgctcct ggccgtcgtc gccgcggcag gctttctgac gtccccagtc
     1861 gagacgcaat ccgccggaga cgggggtgag tgggcggaaa aggcgcggct cgggttcgag
     1921 cgcacggaag ccgccgtcgc cgtcgtcgcg ggccgggtgt atgtcgtcgg cggcatgtcc
     1981 cgcgggcagg actcggacac gctgacgcag gagtacgacc cgaagaccga ccgctggcgc
     2041 gagcgggcgc cgatgcctcg gccgctcagc catccgggcg ccgccgtgct gaacggcaag
     2101 atctacgtcg tcggcggctt cctccggaac gttcacctgg acgcgcaaag cgacgcgttc
     2161 gcatacgacc cggcgacaga tcgatgggag atgctg
//
LOCUS       DBNGSH010000343         6477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3085150, whole genome shotgun sequence.
ACCESSION   DBNGSH010000343 DBNGSH010000000
VERSION     DBNGSH010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3085150"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="AC16CY_08060"
     CDS             <1..1218
                     /locus_tag="AC16CY_08060"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353779.1"
                     /translation="YRSMRKDAEEIDGRVGETFSGIRVVRAFGREIRELIEYLRGRHT
                     LLRKEMFAHRREMVIWTSWGLLLGAVNVVIVWYGGYLNIVGGASVGDIMAFQWYTFLL
                     LNPVWNIVNSFSELQRSLAAMERVFQVLAMEDDKPDRADARDAPRLVQEIRFEDVEFE
                     YREGRPVVLDFNVVVPGGSVVALVGRSGAGKTTVTDLVARFHDPTKGRILLNGSDIRG
                     FRLRTYRDLLALVQQDVFLFDGSVRDNIAYGRHDATEAEVEDAARRANAHEFIVKLPE
                     QYETFVGERGVKLSGGQQQRLAIARAILSSPQILILDEATSNLDTESEQLIQASMAEL
                     LAGRTTFVIAHRLSTIRRADLILLMEDGRIIERGTHEELMRARGEYYGMVIRQMESHG
                     EYAGGAMAEEVLR"
     gene            1224..2291
                     /locus_tag="AC16CY_08065"
     CDS             1224..2291
                     /locus_tag="AC16CY_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR3353780.1"
                     /translation="MLDASIVGSLPKPSWLAQPGMLRAPWRLSGPELRDAQDDAVRMA
                     ILEQEEAGLSTVTDGEQRRRHYIWGFLEGLRGTDTETLAKAHSRGGRYAPATEVARIV
                     GPVKRTAPVFLDACRFARAATRRRLKVTLPGPMTIVDSVADEHYGTDRKTLAMRFAAI
                     LNAEARDLANAGADVIQFDEPCFNIYLSEVEAWGIEALEACMDGVAATRAVHICYGYG
                     TPAVLAWKKSNTDWGHYGVTLPLLARSAVHQVSVECAASGVDLSVLRGLGDKDVLFGA
                     IDVGTEQVETPDVVADRIRRALDHVPAARLFPCTDCGLVPRSREASRGKMRALAEGAA
                     LVRSEIALVGADGRRPTGRIS"
     gene            2410..3264
                     /locus_tag="AC16CY_08070"
     CDS             2410..3264
                     /locus_tag="AC16CY_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353781.1"
                     /translation="MRYRFTGAAVAMAIAAAAWLTTTPTSGQARGQGQAAAPAAAQGR
                     GQARPTTIAGRPNFNGVWQAIGTAHWNLEDHSAAATPFWQLGAMFAIPAGQSVVVGGT
                     IPYLPAGLKRREENMKGWPKSDPEAKCYMGGIPRSNYMPYPFQIVQGQRDILFVYEYA
                     SSNRIVHMEKPSEPPVDSWMGWSNGKWDGNTLVIEVTGHNDQTWFDRAGNNHTEALKV
                     TERFTLQGENHIQYEATLEDPKTFSKPWQIRMPFYRRMEPNAQLLEYKCVEFSEELLY
                     GDLKKKTE"
     gene            3441..3863
                     /locus_tag="AC16CY_08075"
     CDS             3441..3863
                     /locus_tag="AC16CY_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3353782.1"
                     /translation="MRTKLFIAAGAALLLAVAGVQVSAHHSFAAEFDAKRPVKLRGTV
                     VKMEWINPHSWIHIDVKDPATGKVERWMVEGGAPNALLRRGWNKNSLTEGTEILVEGF
                     QAKDGALRANGRDITFPDGKKLFVGSSGIGAPDERPEK"
     gene            3975..4253
                     /locus_tag="AC16CY_08080"
     CDS             3975..4253
                     /locus_tag="AC16CY_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HIR3353783.1"
                     /translation="MPRISSSSTLEAVSAARWRIAISLTAAMMAVYFGFVLLVAFNKP
                     LLGTLVARGLSLGMLLGALVILVAWALTWIYVRWANTHYDSSLAGLRK"
     gene            4250..5791
                     /locus_tag="AC16CY_08085"
     CDS             4250..5791
                     /locus_tag="AC16CY_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR3353784.1"
                     /translation="MNSALGEPNAIAITFFLLFIALSLGITIWAARKTRTAEHFYAAG
                     RSVTAFQNGLALAGDYMSAASFLGIAGLVALSGFDGLIYSIGWLVGWPIVMFLIAEPL
                     RNLGRYTFADVVAARLRQTPIRIAASIGSLAVIAFYLIAQMVGAGNLIRLMFGLPYEA
                     AVAAVGGVMLAYVLFGGMIATTWVQIVKAVMLLGGASALALFVLARFSFNPLELFARA
                     SEVYGDGVLSPGRLVTDPIDAVSLGLALMLGTAGLPHILMRFYTVPDARTARTSVGYA
                     TAFIGFFYLLTFVLGFGAMVIIGRDAITAIDAGGNMAAPLLAEGVGGTPFLGFISAVA
                     FATILAVVAGLTLSGAATLSHDLWVNVVRGAHADQREQLVVARMATVLLAIVSIVLGI
                     TFKGQNVAYMVGLAFAIAASANFPALVLSIFWKGLTTAGAQFSMILGTVFTLVLIYLS
                     PAIQIDILGHSSAWFPLRNPGIVTIPLSFVVAIGVSVLRPQASEAKRFAELERRLHLG
                     VEHAD"
     gene            5862..>6477
                     /locus_tag="AC16CY_08090"
     CDS             5862..>6477
                     /locus_tag="AC16CY_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3353785.1"
                     /translation="MAVVVVLMRRGPALPSPGSQAYEDVSRAFYWGLSALEVGLLDDA
                     RQQFSKAAELVPEEPASWADLGLTQLRLGELEAAAAPIERALALAPGNGDIVLLAAKM
                     EAARGRLDEAVARLRQAVTLDAGGLRSRFALAEELQRLNTAEADVEALALLDAVLERA
                     PNNLAALVDRARIAARRGDEPRLRGSRDALAQLTSSWSPQAIEQF"
BASE COUNT         1037 a         2082 c         2224 g         1134 t
ORIGIN      
        1 taccggtcga tgcgcaagga cgccgaggag atcgatgggc gcgtcggcga gacgttctcg
       61 ggcatccgcg tcgtccgggc cttcgggcgc gagatacgcg agttgatcga gtacctgcgc
      121 ggcaggcaca cgctgctgcg aaaggaaatg ttcgcgcacc gccgcgagat ggtcatctgg
      181 acttcctggg gcctgctgct cggagcggtc aacgtggtga tcgtctggta cggcggatac
      241 ctcaacatcg tcggcggcgc gtcggtcggc gacatcatgg cgttccagtg gtacacgttc
      301 ctgctgctca accccgtgtg gaacatcgtc aactccttct ctgagctgca gcggtcgctg
      361 gctgcgatgg agcgcgtctt ccaggtgctg gcgatggagg acgacaagcc cgaccgggct
      421 gacgcgcgcg acgcgccgcg cctcgtgcag gagattcgct tcgaggacgt cgagttcgag
      481 taccgcgaag gccgtcccgt ggtcctcgac ttcaacgtcg tcgtgcccgg cgggtccgtc
      541 gtggcgctgg tcggccggag cggggccggg aagacgacgg tcacggatct ggtggcgcgg
      601 tttcacgatc cgacgaaagg gaggatcctg ctcaacgggt cggacatccg cggcttccgg
      661 ctgcgcacgt accgcgacct gctcgcgctt gtccagcagg acgtgttcct cttcgacggc
      721 tcggtgcgcg acaacatcgc gtacggccgt cacgacgcca ccgaggcgga agtcgaggac
      781 gcggcacggc gggccaacgc gcacgagttc atcgtcaagc tgccggagca gtacgagacg
      841 ttcgtcgggg agcgcggcgt caagctgtcg ggcgggcagc agcagcgcct ggcgatcgcg
      901 agagcgattc tgtcgtcgcc ccaaatcctc atcctcgacg aggcgacgag caacctcgac
      961 accgagagcg aacagttgat ccaggcgtcg atggccgaat tgctcgccgg ccggacgacg
     1021 ttcgtcatcg cccaccgtct gtccacgatc cggagagcgg acttgatcct gctgatggag
     1081 gacgggcgca tcatcgagcg agggacgcac gaggagctga tgcgcgcgcg gggcgagtac
     1141 tacggcatgg tgatccggca gatggagtcg cacggcgagt acgccggcgg ggcaatggca
     1201 gaagaggtgc tgcgttagcc gccatgctcg acgcatcgat cgtcggcagc cttcccaagc
     1261 cctcctggct cgcgcagccc ggcatgctca gggcgccgtg gcgcctctcc ggtcccgagc
     1321 tccgcgatgc gcaggacgac gccgtgcgga tggccatcct ggagcaggag gaggccgggc
     1381 tgagcacggt gaccgacggg gagcagcgcc ggcggcatta catctggggc ttcctcgagg
     1441 ggctgcgcgg gacggacacc gagacgctcg ccaaggcgca ctcccgcggc ggccggtacg
     1501 cgccggcaac ggaggtcgca cgaatcgtcg ggccggtgaa gcgcacggcc ccggtgtttc
     1561 tcgacgcgtg ccgcttcgcg cgggcggcca ccaggcgccg gctcaaggtg accctgcccg
     1621 gccccatgac catcgtcgac agtgtggccg acgagcacta cggcaccgac cggaagacgc
     1681 tggccatgcg ctttgccgcg atcctcaatg ccgaggctag ggatctggcc aacgccggcg
     1741 ccgacgtgat tcagttcgac gagccgtgct tcaacatcta cctcagcgag gtggaggcgt
     1801 ggggaatcga ggcactcgag gcctgcatgg acggcgtggc agcaaccagg gccgtgcaca
     1861 tctgttatgg atacggcacg ccggccgtat tggcgtggaa gaagtcgaac acggactggg
     1921 gacactacgg agtcacgctc ccgctgctgg ccaggagcgc cgtccaccag gtgtcggtgg
     1981 aatgcgcggc ctcgggtgtc gatctctccg tgttgagggg gctcggagac aaggatgtgc
     2041 tgttcggagc gatagacgtc gggaccgagc aggtggaaac gcccgacgtc gtggcggacc
     2101 ggattcgtcg cgcgctcgat cacgtgcccg ccgcccgcct gtttccctgc accgactgcg
     2161 ggctggtacc gcgcagccgc gaggcgtctc ggggtaaaat gcgcgcactc gccgaaggcg
     2221 ccgcgctcgt ccgatcggag atcgcgctgg tgggggcaga tggcaggcga ccgacgggcc
     2281 ggatttcttg acagaacgtc tttgactctg gtaaagctcc gtccggcgga gtcatctttt
     2341 agacacattt cgagcgaata cccgcgagga cgacgacccg tcgacccgtg aggagaggag
     2401 acctggcaga tgcgataccg gttcacaggc gcagcggtgg cgatggcgat agcggcggca
     2461 gcgtggctga ccacgacgcc gacctcgggg caggctcgag gtcagggtca ggccgccgcg
     2521 cccgctgcgg ctcaggggcg cggtcaggca cgtcccacga ctatcgctgg tcgtccgaac
     2581 ttcaacggcg tctggcaggc gatcggcacc gcccactgga acctcgagga tcactcggct
     2641 gccgcgacac cgttctggca gctcggcgcg atgttcgcga ttccggccgg gcagagcgtg
     2701 gtcgtgggcg gcacgattcc ttacctgccg gcgggactga agcggcggga agagaacatg
     2761 aagggctggc ccaagtccga cccggaagcg aagtgctaca tgggtggcat tccgcgctcg
     2821 aactacatgc cctatccgtt ccagatcgtc cagggacagc gcgacattct cttcgtgtac
     2881 gagtacgcga gctcgaatcg catcgtgcac atggagaagc cgtccgagcc gccggttgat
     2941 agctggatgg gctggtccaa tggcaagtgg gatggcaaca cgctcgtgat cgaggtcacc
     3001 ggacacaacg accagacctg gttcgaccgc gcggggaaca accacaccga ggccctcaag
     3061 gtgaccgagc ggttcacgct tcagggcgag aaccacattc agtacgaagc cacgctcgag
     3121 gatcccaaga cgttctcgaa gccgtggcag attcggatgc cgttctaccg ccgcatggag
     3181 cccaacgcgc agctcctcga gtacaagtgc gtggagttct ccgaggaact gctctacgga
     3241 gatctgaaga agaaaaccga atagcggaga agggcgaaaa ccgggaactt caacggctgg
     3301 tgccgattac ggctattccg gtgctacact ggacgctgac gggcgccgtg cgtgcgtcgt
     3361 atacttgtgg caagcaatcc cgggtggggc cggcagtaga attcggctaa acgtcctgac
     3421 tcaggtagga gatgaattcg atgcgaacga aactcttcat tgcggccgga gccgcgcttc
     3481 tgctggcggt cgctggtgtt caggtgtcag cgcaccactc ctttgcggct gaattcgacg
     3541 caaagcgccc ggtcaagctg cggggcaccg tcgtcaagat ggagtggatc aacccgcact
     3601 cctggattca catcgacgtg aaggatccgg ccaccggtaa ggtggagcgg tggatggtcg
     3661 aaggcggcgc gcccaacgcg ttgctgcgtc gcggctggaa caagaactcg ctgaccgagg
     3721 gcacggaaat cctcgtcgag gggttccagg ccaaagacgg cgccctccgc gcgaacggcc
     3781 gtgacatcac gttcccggac ggcaagaaac tgttcgtcgg ctcctcgggc atcggcgcgc
     3841 ccgacgagag accggagaag taggctcttc caggttctca gctagccggc ttccatcgtg
     3901 tcgaacgccc gttcgatgct gggagccggc cgttcgttcg ctcatccgcc gtgcggggcg
     3961 cggcgaccct agccgtgcct cgcatatcct cctcttccac gctcgaagcc gtgtcggccg
     4021 cacggtggcg aatcgcgatc tcgctgacgg cagcgatgat ggccgtgtac ttcggcttcg
     4081 tcctcctggt ggcattcaac aagccgctcc tcgggacgct ggtcgcgcgg gggctcagcc
     4141 tgggcatgct gctcggtgcg ctcgtgattc tcgtcgcgtg ggcgctgacg tggatctacg
     4201 tccggtgggc caacacgcac tatgactcct cgctggcagg gctgaggaaa tgaactccgc
     4261 cctcggcgaa ccgaacgcca tcgccatcac gttcttcctg ctcttcatcg cgctgtcgct
     4321 cggcatcacg atctgggccg cgaggaagac gaggacggcc gagcacttct acgccgccgg
     4381 ccgcagcgtc accgcctttc aaaacgggct ggcgctcgca ggcgattaca tgagcgccgc
     4441 cagcttcctc ggcatcgcgg gcctcgtggc gctgtctggt ttcgacgggt tgatctactc
     4501 gatcggctgg ctcgtgggct ggccgatcgt catgttcctc atcgcggagc cgttgagaaa
     4561 cctgggccgg tacacgttcg ccgacgtcgt ggccgcgcgc ctgcgccaga cgccgatccg
     4621 cattgccgcc tcgattggat cgctggccgt catcgcgttc tacctgatcg cgcagatggt
     4681 cggcgcgggg aacctgatcc ggctcatgtt cggcctgccg tacgaagcgg ccgtggctgc
     4741 cgtcggcgga gtcatgctcg cgtatgtgct ctttggcggg atgatcgcga cgacgtgggt
     4801 gcagatcgtc aaggccgtca tgctgctcgg aggcgcctcg gcgctcgccc tgttcgtcct
     4861 ggcccggttc agcttcaacc cgctggagtt gttcgcgcgg gcgtccgagg tgtacggcga
     4921 cggtgttctg agccccgggc gtctggtgac cgatccgatc gacgccgtgt cgctgggtct
     4981 tgcgctgatg ctcgggaccg cgggcctgcc gcacatcctc atgcgtttct acaccgtccc
     5041 ggacgcgcgc acggcgcgga cctcggtggg atacgcgacg gcgttcatcg gctttttcta
     5101 tctgctgacg ttcgtgctcg gcttcggcgc gatggtcatc atcgggcgcg acgcaatcac
     5161 ggcgatcgac gcgggcggga acatggccgc gcccctgctc gcggagggcg tcggcggcac
     5221 gccgtttctc ggcttcattt cggcggtggc gttcgcgacg atcctggcag tcgtcgcagg
     5281 gctgaccttg tctggagcgg cgacgttgtc gcacgatctg tgggtcaacg tcgtgcgcgg
     5341 cgcgcacgcc gaccagcgcg agcagctcgt cgtggcgcgg atggccaccg tgctgctggc
     5401 catcgtctcg attgtgctcg gcatcacgtt caagggccag aacgtggcat acatggtggg
     5461 tctcgcgttt gccatcgcag ccagcgccaa tttccccgcg ttggtgctct ccatcttctg
     5521 gaagggcctg acgacggccg gcgcgcagtt cagcatgatc ctcggcacgg tgttcaccct
     5581 cgttctgatc tacctgtcgc cggccattca gatcgacatc ctcggacact ccagcgcctg
     5641 gttcccgctt cgcaaccccg gcatcgtgac cattccgctg tccttcgtcg tggcgatcgg
     5701 cgtttcagtg ctgcgtcccc aggcgtccga ggcgaaacgc ttcgcggagc tcgagcgacg
     5761 gctgcacctg ggcgtggagc atgccgactg agcccgctcg cacccttgca ccctcgcact
     5821 ctcgcacctt ccttcttgtt gttgtagtcc tgcttgccct catggccgtc gtcgtcgtgt
     5881 tgatgcggcg cggaccggcc cttccgtcgc cggggtccca ggcgtacgaa gacgtgtcgc
     5941 gcgcgttcta ctgggggctc tccgcgctcg aggtcggcct tctcgacgat gcgcggcagc
     6001 agttcagcaa ggccgcggag ctcgttcccg aagagcccgc gtcgtgggcc gacctggggc
     6061 tgacgcagct gcggctgggg gagctcgagg cggccgccgc gcccatcgaa cgcgcgcttg
     6121 cgctggcgcc cggcaacggt gacatcgtgc tgcttgccgc aaaaatggaa gccgcgcgcg
     6181 ggcgattgga cgaggccgtc gcgcggctgc gccaggcggt gacgctcgac gccggagggc
     6241 tgcgatcgcg cttcgcgctc gccgaggagc tgcagcgcct gaacacggct gaagcggacg
     6301 tcgaggcgct cgccttgctc gacgccgttc tcgagcgcgc cccgaacaac ctggcggcgc
     6361 tcgtcgatcg cgccaggatt gcggccaggc gcggcgacga accccgtctc cgcggttccc
     6421 gtgacgcgct cgcgcaattg acgtcgagct ggtcgcccca ggcgatcgag cagttca
//
LOCUS       DBNGSH010000344         2391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4273215, whole genome shotgun sequence.
ACCESSION   DBNGSH010000344 DBNGSH010000000
VERSION     DBNGSH010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4273215"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2210)
                     /locus_tag="AC16CY_08095"
     CDS             complement(<1..2210)
                     /locus_tag="AC16CY_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353786.1"
                     /translation="MRRIWCRVRAVLHRVWDDGARDQELSEELRAFVELDAASKIRSG
                     MTPADARRAALVELGGAEQVKERVREVRAGARWESILRDIRYAVRSLGRSPGFSFSVI
                     GSLSLGLAATTVAFAFINGALLRPFPGVRDQDRLVTLGILERTPFGSTLPLTAMADYP
                     DAFRALREGMTSLEGLASVTESDVAVTLPQPRSFPAEFVSPNYFDVLGVQPQIGRTFA
                     PEGGQAESAVAIIGHALWTQEFGRDPSVAGRPIQVGGQIVQIVGVAPQGFRGTAQSPT
                     QTGVELWLPISLVDRVGDPLSFGRGNIVLLNDTPGERLIRYVGRMRNGVRADLVETEL
                     AVVARRVVTPANDLTAPVVTEVSSLSRLNDRTNAGQIVAIIVSIPLLVLAIACVNAAN
                     LLLVRASRRRREMALRLALGASRLRLVRQLVIESLVLSIGAAVLALPLAWWGLQWVAA
                     FMTIPMPLDGTVAAGALVTAFLTALGFGLVPALHAAGQHPSAAVGTSPAGSGGTPAES
                     RGRRVLVAGQVALSLALLATGFQLTSALESLAEPPGTDPDRLLLASFNLGHLRFSPAE
                     SDAFYAALLDGVSRLPGVGAAGLSSRNLGQGWDPFFDPANAIRVDPGNGPDGRLLRAS
                     ASTGGDYFKALGLDLVQGREFVAADRREIPDVAIVTESLASQLFKNAPLGRSLRASTP
                     FPGAVPADLRIVGVVESPLGLSGEEVQAVFFPSHFPSPIQRGTARTLHVRADGPA"
BASE COUNT          437 a          821 c          813 g          320 t
ORIGIN      
        1 gcaggaccgt cggcacggac gtgaagcgtg cgcgcggtgc cacgctgaat tggcgacggg
       61 aagtgcgagg ggaagaaaac cgcctggact tcctcgccgg acagtcccag gggcgactcc
      121 acgacaccca cgatccggag gtcggccggc actgcgccag ggaaaggcgt cgacgcacgc
      181 aggctccggc cgagtggggc attcttgaag agttgagacg ccagactttc ggtgacgatc
      241 gccacgtcgg gtatctcgcg tcgatcagcc gcgacaaatt cccggccttg cacgaggtcg
      301 agccccaggg ccttgaagta gtcgccaccc gtagaagcac tggcccggag gaggcgcccg
      361 tccggcccat tgccgggatc cactcgaatg gcattggcgg ggtcgaagaa ggggtcccaa
      421 ccctgcccca gattgcggct cgacagccct gcggcgccga ctccgggaag ccgtgaaaca
      481 ccgtcaagaa gcgccgcata gaatgcgtcg ctctcggcgg gtgagaagcg gagatgcccc
      541 aggttgaacg acgccagcag cagacggtcc gggtccgtgc cgggcggctc cgccagagat
      601 tccagcgcgg acgtcaactg aaacccggtg gcgagcagag ccagtgagag tgcgacctga
      661 ccggcgacga ggacccggcg gccgcgggac tcggcaggcg tgccgccgct ccccgcggga
      721 gatgtgccga ccgcagcgga cgggtgctgg ccggcggcgt gaagcgccgg cacgagtccg
      781 aatcccagag ccgtcaggaa cgcggtcacg agtgcgcccg ccgcgaccgt cccgtcgagc
      841 ggcatgggaa tcgtcatgaa cgccgcaacc cactgcaggc cccaccacgc cagcggcagc
      901 gcgaggaccg ccgcgccgat ggacaggacg aggctctcga tgaccagttg ccggacgagc
      961 cggagccggg acgcgcccag cgccagacga agcgccattt cccggcggcg ccgagaggct
     1021 cgcacgagga gaagattcgc cgcgttgaca caggcgatcg cgagcacgag aaggggaatg
     1081 gacacgatga tggcgacgat ctgtccggca ttggtcctgt cgttgagtct cgacagactg
     1141 gacacttcgg tgacgaccgg cgccgtcagg tcgttcgcgg gcgtgacaac ccgccgggca
     1201 acgacggcga gctcggtctc gacgaggtcg gcgcggacac cgttccgcat gcggcccacg
     1261 tatcgaatca ggcgctcacc gggcgtatcg ttgaggagga cgatgttgcc cctcccgaac
     1321 gaaagcggat cgccaactcg atccacgaga ctgatcggca gccagagctc gacgcccgtc
     1381 tgagtcgggc tctgggccgt gccccgaaat ccctgcggcg cgacgccgac gatctggacg
     1441 atctgccctc ccacctggat gggccggccg gccactgagg gatcgcgtcc gaactcctgc
     1501 gtccacagcg cgtggccgat gatcgcgacg gcggactcgg cctggcctcc ctcgggtgca
     1561 aaggtccgtc cgatctgcgg ctgtacgccg agtacgtcga agtagttcgg tgacacgaat
     1621 tcggccggga aggagcgggg ctgaggcagg gttaccgcca cgtcggattc ggtgacgctg
     1681 gccagacctt cgagactggt catcccctcg cggagcgcgc gaaatgcgtc cgggtagtcc
     1741 gccatcgcgg tcagggggag tgtcgatccg aagggggttc tttccagaat cccgagcgtc
     1801 acgaggcgat cctgatccct gacacccgga aacggacgaa gcagcgcgcc gttgatgaac
     1861 gcgaacgcca cggtcgtcgc cgccaggccc aggctgagac tcccgatcac cgagaacgag
     1921 aacccgggtg accggccgag actgcgtacg gcgtaccgga tgtccctgag aatgctttcc
     1981 cagcgagctc cagcccggac ttcccgaaca cgctccttga cctgttcggc gccccccaac
     2041 tcgacgagcg cggcgcgccg tgcatccgcc ggcgtcattc ccgaccggat cttcgacgcg
     2101 gcgtcgagct cgacgaacgc gcggagctcc tcactgagct cctggtccct cgcgccgtcg
     2161 tcccacaccc ggtgcagcac cgcccgcacg cgacaccata tgcgtctcat gcttcctact
     2221 cctgtcgcag caccacgttg ggatccagcc gggcggcccg ccacgcgggc aggatgctgg
     2281 ccaggagcat cgccgctgca agcagcgccg ccgagccgcc cagcgccgcg aacccgactc
     2341 cggggacacc gaacacctcc gcctggatga cgaatccggc cgcgacggcg a
//
LOCUS       DBNGSH010000345         2838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2066343, whole genome shotgun sequence.
ACCESSION   DBNGSH010000345 DBNGSH010000000
VERSION     DBNGSH010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2066343"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="AC16CY_08100"
     CDS             complement(<1..652)
                     /locus_tag="AC16CY_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv0361 family membrane protein"
                     /protein_id="HIR3353787.1"
                     /translation="MRFPLLLLALALVLSCGQPTAPAIRDEDAVRQAYESYRGAMSGG
                     DLARLTTLVSRERAKELSAPDAAETLQAARAFYPEGAQITRVQLNGREATLELTARVQ
                     EGTAAGTVRLVKEDEAWKVSTEDWQVTISIRADGSPDSTAELPSDTVRPADYSTLLGT
                     WQGGEGGSREWTFTFADDFVVSAEHRSGSRYHGQAAIFWDLGTVSGTIRVPPGWSVL"
     gene            740..1246
                     /locus_tag="AC16CY_08105"
     CDS             740..1246
                     /locus_tag="AC16CY_08105"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3353788.1"
                     /translation="MSHTEGFQMNRMATGSLAVLAAFVSAGPLLAHHSLASFDTQTAV
                     RVKGTIAQVHYINPHSFIYVDEKREDGQIVRRWAVEGPSLLQLNRQGFAKDALKPGDV
                     VEVCGYLPKEALSWQIASADPTAASLAGRLLNAEVLTMPDGTQRSWGDYGFHKCFAPG
                     YTDQHSSR"
     gene            complement(1272..1931)
                     /locus_tag="AC16CY_08110"
     CDS             complement(1272..1931)
                     /locus_tag="AC16CY_08110"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006307 - DNA alkylation repair [Evidence
                     IEA];
                     GO_process: GO:0035552 - obsolete oxidative
                     single-stranded DNA demethylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HIR3353789.1"
                     /translation="MSGLLAELPRQREPVQVAPGAVWLKNWLSLDDQARLAGQCRAIL
                     DGPSGGYTPTVRGGGKMRVRMTCLGRHWNPLTYTYESTRADHDGAPVKPVPDDWVALA
                     SSLALEAGFVCRPDICLINFYGDDGRMGLHQDKDESQASLESGKPVVSISLGDTARFL
                     FGGVKRRDPVETLLLESGDAFIFGGAARLRYHGVSRIIPHTSPPAVCGGGRFNLTFRQ
                     Y"
     gene            complement(1974..2549)
                     /locus_tag="AC16CY_08115"
     CDS             complement(1974..2549)
                     /locus_tag="AC16CY_08115"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3353790.1"
                     /translation="MDDGTVVVEAIVSERMTVWEYLGGEETTRPRELAYGILREPPAP
                     GYSHQVIVGRIYRLLDRHVRRCAAGQVAISPLDVVLDRERALVVQPDVMFISEARLGI
                     CKERIWGAPDLVVEVLSPATRRHDCTMKVAWFKAYGVRECWLVDPVARTIDVLDCAAP
                     DSASRTFERDEIVQSTVLPRLRLRVDAVFAR"
BASE COUNT          510 a          923 c          930 g          475 t
ORIGIN      
        1 cgagcacgct ccagccaggc ggcacgcgga tggtcccgga cacggtgccg aggtcccaga
       61 agatcgccgc ctgtccgtga tagcggctgc cggacctgtg ctcggccgat acgacgaagt
      121 cgtcggcaaa ggtgaacgtc cactctcgcg acccgccctc gcctccctgc cacgtgccga
      181 ggagcgtcga atagtcggcg ggccgtacgg tatcggacgg gagttccgcc gtggaatcgg
      241 gcgagccatc ggcccggatg ctgatcgtga cctgccagtc ctccgtgctc accttccagg
      301 cctcgtcttc cttgacgaga cgaacggtgc ccgcggccgt gccttcctgc acgcgcgcgg
      361 tgagctccag cgtcgcttcg cggccgttca actggacgcg ggtgatctgc gcaccttcgg
      421 ggtagaaggc gcgggccgcc tggagggtct cggccgcatc tggagcgctc agctcctttg
      481 ccctctcccg cgagacgagc gtcgtcaacc tggcgaggtc gccgcctgac atcgcccctc
      541 gatagctctc gtaggcctgt cgcaccgcat cctcgtccct gatggcggga gcggtcggct
      601 ggccgcacga caggacgagc gcgagcgcca gcaggagaag ggggaatcgc acgcgcatac
      661 cttccctatc ggcgccttga tttgatggat ttagccggca ggtgacaatt gggtaacacg
      721 acaactctat gttgcccaca tgtctcacac cgaggggttc cagatgaaca ggatggcgac
      781 gggatcgctc gcggttctgg cggcgttcgt ttcagcgggt ccgttgctgg cccatcattc
      841 gctggcgagt ttcgacaccc agacggccgt ccgcgtgaag ggaacgatcg cgcaggtcca
      901 ctacatcaat cctcacagct tcatctatgt ggacgagaaa cgcgaggatg gacagattgt
      961 tcgccggtgg gccgtcgaag gcccgtcgct tcttcaactc aaccggcaag ggtttgcaaa
     1021 ggatgcgctg aaacccggcg acgttgtcga agtgtgcggg tacctgccca aggaagccct
     1081 ctcgtggcag attgccagcg ctgacccgac tgccgccagc ctggctggcc gattgctcaa
     1141 tgccgaagtg ctgacgatgc ccgatggaac acaacggagc tggggcgact acggattcca
     1201 caaatgcttt gcgcccggct acaccgatca gcattcgtcg aggtgatgcc agcgcgtgcg
     1261 tcgagcgcgg ttcagtactg cctgaacgtg agattgaatc gcccgccgcc gcacacggcc
     1321 gggggcgagg tgtgcgggat gatgcgggac acgccgtgat accggaggcg cgcggcgccg
     1381 ccgaagataa acgcgtcgcc tgattcgagc agcagcgtct cgaccggatc ccgccgcttg
     1441 acgccgccga acaggaatcg cgccgtgtcg ccaagcgaga tggacacgac cggctttcct
     1501 gactcgagcg acgcctggct ctcgtccttg tcctggtgca gccccatgcg gccgtcgtcg
     1561 ccgtagaaat tgatcaggca gatgtcgggc cggcagacga acccggcttc gagcgcgaga
     1621 gacgaggcga gcgcaaccca gtcatcaggt acgggcttca cgggcgcgcc atcgtgatcg
     1681 gcgcgggtgg attcgtacgt gtaggtgagc ggattccagt ggcggcccag gcacgtcatc
     1741 cgcacacgca tcttgccgcc accgcgaacc gttggtgtgt atccgcccga gggaccgtcc
     1801 aggatcgcgc ggcactgtcc tgcgagcctc gcttgatcgt cgagcgacag ccagttcttg
     1861 agccacaccg cgcctggcgc gacctggacg ggctcgcgct gtcgaggaag ctcggcgagg
     1921 aggccggaca tcatggacat tatgcccgcc cgggactcga ggtcgcgcgg tcgtcagcgt
     1981 gcgaacaccg cgtccacgcg caggcggaga cgcggcagca cggtcgattg aacgatttcg
     2041 tccctctcga acgtccgtga tgcggagtcg ggtgcggcac agtccaacac gtcgatggtg
     2101 cgggcaacgg ggtcaacgag ccagcactcc cgcacgccat acgctttgaa ccacgccacc
     2161 ttcatcgtgc agtcgtgtcg ccgcgtggcc ggggacagca cctcgacaac gaggtccggg
     2221 gcaccccaga tccgctcctt gcagatcccg agcctcgcct ccgaaatgaa catcacgtcc
     2281 ggctgaacga ccagcgcgcg ttcgcggtcg agcacgacgt cgagaggaga gattgcaacc
     2341 tggccagccg cgcaacggcg cacatggcgg tcgagcagcc tgtagatccg tccgacgatc
     2401 acctggtgcg agtacccggg cgcgggtggc tcacgcagga tcccgtaggc cagctcacgg
     2461 ggccgtgtcg tctcctcgcc gccgaggtac tcccacaccg tcatccgctc ggacacgatc
     2521 gcctcgacga cgacggtgcc atcgtccacg cgcggatgtc aaggctacgg acagaagccg
     2581 acgcagggcg atcaatcaga taatctgatt tacgtgaaga tgacggtcac cgacttccag
     2641 agacgatccc gcctggcacg ggaggcagcc gaccgtggag acacggtcat cgtgaccggt
     2701 gagcacgggg actaggtgtt gtttctaggg acgttgttca ctgtgctgcc cttgggaagc
     2761 ggataacggg cggccgatat gcgaggctcg catgcggccg ctctgcattg taaaaccgca
     2821 gccacggccg caacgcgc
//
LOCUS       DBNGSH010000346         1510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1557012, whole genome shotgun sequence.
ACCESSION   DBNGSH010000346 DBNGSH010000000
VERSION     DBNGSH010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1557012"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /gene="atpA"
                     /locus_tag="AC16CY_08120"
     CDS             complement(<1..1261)
                     /gene="atpA"
                     /locus_tag="AC16CY_08120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3353791.1"
                     /translation="MANLIQDIEAQIAGAKTTALKRSIGIIREIGDGVARVEGLGDVM
                     LNEMLDLGNGITGIALSLEETEVGVIILGDYTSLEEGNEVTATGKLLQVPVGKGLLGR
                     VVNMLGEPVDGKGPIASDVAYPVEKVAPGIMRRKQVSQPVQTGIMAIDAMIPIGRGQR
                     ELIIGDRSTGKTTICIDTIISQGRLNHEAEAAGNKDYRPLYSIYVAIGQKQSTIARII
                     AVLEEAGAMPYTIIIASPAADSATNQYLAPFAGAAMGEWFMDNGMDALIVYDDLSKHA
                     VAYRQVSLVLKRPSGREAYPGDVFYLHSRLLERAARVGEQYGNGSLTALPIIETQAGD
                     VSAYIPTNVISITDGQIYLETDLFYQGIRPAISVGLSVSRVGSAAQLKAMKQVAGRLK
                     GDLAQFRELAAFAQFGSDLDATTQSRID"
     gene            complement(1301..>1510)
                     /locus_tag="AC16CY_08125"
     CDS             complement(1301..>1510)
                     /locus_tag="AC16CY_08125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR3353792.1"
                     /translation="SAKVESATPLPDDVRASIEGSLTRIHGRGLTTSFSGNAALLGGV
                     RITVGSDVYDGSIRGRLAALEAKFQ"
BASE COUNT          224 a          510 c          495 g          281 t
ORIGIN      
        1 gatcgattct cgactgcgtc gtggcgtcga gatccgaccc gaactgcgcg aaggcggcca
       61 gctcgcggaa ctgcgccagg tcgcccttca gccggccggc gacctgcttc atcgccttga
      121 gctgcgccgc cgacccgacg cgcgacaccg acaagccgac ggagatcgcc gggcggatgc
      181 cctggtagaa caggtccgtc tcgagataga tctggccgtc ggtgatcgag atgacgttgg
      241 tcgggatgta ggcggacacg tcgccggcct gcgtttcgat gatgggaagc gcggtcagcg
      301 aaccgttgcc gtactgctct cccacgcgcg ctgcacgctc gagcagacgg ctgtgcaggt
      361 agaacacgtc gccgggatag gcctcacggc cggacggccg cttcagcacg agcgagacct
      421 gacggtaggc gacggcgtgc ttggacagat cgtcgtagac gataagcgcg tccatgccgt
      481 tgtccatgaa ccactcgccc atcgccgcgc cggcaaacgg cgccaggtac tggttggtgg
      541 cggaatccgc cgccggggag gcgatgatga tcgtgtacgg catcgcgccg gcctcctcga
      601 gcacggcgat gatgcgcgcg atcgtggact gcttctgccc gatggcgacg tagatgctgt
      661 agagcggacg atagtccttg ttcccggccg cctccgcttc gtggttcagc cggccctggc
      721 tgatgatggt atcgatgcag atcgtcgtct tgccggtcga acggtcgccg atgatgagct
      781 cgcgctggcc gcggccgatc gggatcatcg cgtcgatggc catgatgccg gtctgcacgg
      841 gctggctgac ctgcttgcgc cgcataatgc cgggcgcgac cttctcgacc gggtacgcca
      901 cgtcgctggc gattgggccc ttgccgtcta ccggctcccc gagcatgttg accacgcggc
      961 cgagcagccc cttcccgacc gggacttgca ggagcttccc cgtggccgtg acttcgttgc
     1021 cttcctcgag gctcgtgtaa tcgccgagga tgatgacgcc gacttccgtc tcctcgagac
     1081 tgagcgcaat ccccgtgatg ccgttcccga ggtcgagcat ctcgttgagc atcacgtcgc
     1141 ccagcccctc gacccgggcg acgccgtcgc cgatctcacg gatgatgccg atgctgcgct
     1201 tgagggcggt cgtctttgcc cccgcgatct gcgcttcgat gtcctgtatc aaattcgcca
     1261 tggatctact gccccctggc ccctgccaac tgccccctgc ctactgaaat ttcgcctcga
     1321 gcgctgccag gcgaccccgg atgctgccgt cgtagacgtc gctgccgacg gtgatgcgca
     1381 cgcctcccag cagggcggcg ttgccggaga acgaggtcgt aaggccgcgg ccgtggatgc
     1441 gcgtcaggct cccttcgatg ctcgcgcgca cgtcgtcggg cagcggcgtc gcgctctcca
     1501 ctttggcgct
//
LOCUS       DBNGSH010000347         9736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_368166, whole genome shotgun sequence.
ACCESSION   DBNGSH010000347 DBNGSH010000000
VERSION     DBNGSH010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_368166"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1156
                     /gene="dinB"
                     /locus_tag="AC16CY_08130"
     CDS             77..1156
                     /gene="dinB"
                     /locus_tag="AC16CY_08130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR3353793.1"
                     /translation="MNYRRIIHIDMDAFYASVEQRDRPELRGRPVAVGGDPDRRGVVA
                     AASYEARTFGVRSAMSMAKAVRLCPPLHIVHPDFAKYRAVSQQVFGILRTVTPLVEPM
                     SLDEAYLDVTENAWGATLGMDVARRLKEEIRATTGLTASAGVAPNKFLAKIASGWQKP
                     DGLTVVAPERVESFLQALPVDALWGVGPVTAKKLRDRGIERLVDIRTVEPERLRDTVG
                     SLADWLQQLAKGIDDRPVVSEYEPQSSGSENTFPTDLTDLGEIRVEIADMARSAAAWL
                     EKRELYARTVTIKVRYSDFTTITRSHTEAATRDDGRLAARAVALLEKTDAGQRPVRLL
                     GVSVHNLCDALAPPKPATGRLPFES"
     gene            complement(1186..2313)
                     /locus_tag="AC16CY_08135"
     CDS             complement(1186..2313)
                     /locus_tag="AC16CY_08135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3353794.1"
                     /translation="MLPLMLLVLLADPQAVRPQRPAPPPRDTFFTAQLPLDEMRGKQA
                     VLDTSEGAIVLDLLPESAPNHVAYFIDRARAGAYDGTTFHRVISMGIVQGGDPLSKDP
                     GQSAKYGTGGLGRLRFEPNAEKHTRGAVSAVLVPGNRDSAGSQFFIVVTDQPSLDGQY
                     TVFARVAEGINVAQAISTTPASNSVPAARIEIRKVTIRDKPAPEPEPFASASVEELSS
                     SRAVIATSMGDITLELFADRAPNHVRQFLRLAASGVYDGTAIHRVVRGFVAQGGHLPT
                     RRAPLGERQQGYVRTLQPEFNATPHDRGIVSMARLSDPASATSSFFIVLARATSLDGQ
                     YTVFGRVVGGLDVLDKIEAVPLDGETPVTRIEVTGVRVLRP"
     gene            2345..3277
                     /locus_tag="AC16CY_08140"
     CDS             2345..3277
                     /locus_tag="AC16CY_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR3353795.1"
                     /translation="MSASATPTRSILQYEEFYGFVQSPFTLAPDPRFLYRSSCHEDAL
                     RLLLQSVGRREGIAVLSGDIGTGKTTVCRTLLEQLDMTRFTSLLLNPFLSVEELLREI
                     LLDFGVVSREAVRSGRVTSASKHELVSALHEFLISLVPIGASGVLVIDEAQHLSTQVL
                     EEIRVLSNLETNQNKLLQIILVGQLNLLEILKQPEQRQLDQRITARAVIRPLDRPEVE
                     AYVAHRLGVAKGSAQVRFDAGALDAVQLASGGVPRLINLVCDRTLMLGAQLDLRTMSS
                     AVVGAAAESLALPADKPASPRWWQRLFRRSSSSL"
     gene            complement(3263..4339)
                     /gene="rsgA"
                     /locus_tag="AC16CY_08145"
     CDS             complement(3263..4339)
                     /gene="rsgA"
                     /locus_tag="AC16CY_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR3353796.1"
                     /translation="MTGLEQLGWDDALARAYEAWAGKQDMQPGRILIEFNYIYRAWGP
                     QGETEAVLAGRLKHRAASRGELPAVGDWVAIRKRPDEDRGAIVGVLPRRSWFSRRMAG
                     KVTGEQVVAANVDVVFLVMALDADFSLRRLERYLLLARESGASPVVLLTKPDLCADVE
                     GRVGEVSAIGGGVPVHVVSPKLNLGVDRLVDYLPSGRTGALLGSSGVGKSTIINRLVG
                     RDVQKTKEVRESDSTGRHATSHRELVFLPHGGFIIDTPGMRELQLWDVSEALTETFDD
                     IEALAASCHFGNCRHRGEPRCAVKVAVDDGRIPQERLYSYLKLQDELSHLARQQDERA
                     QLEQKRRGKIGAKALRQHLKSKRG"
     gene            complement(4336..4965)
                     /locus_tag="AC16CY_08150"
     CDS             complement(4336..4965)
                     /locus_tag="AC16CY_08150"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042734.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3353797.1"
                     /translation="MDLTDLGAVGYRAEGATRALIVLGHGAGAGQRHPFMTAVAGGFA
                     ARGIDAVTFDFPYMRARRHVPDRAPVLEKAFLDVVDAARRWSRADRTFIGGKSMGGRM
                     ATHLGAAGVDRLRGIIVFGYPLHPPGKPERLRVDHLPSVKVPVLVVQGERDTFGTPAE
                     LAPVLDTMPAGAEMLPIPGGDHSLTVRGRREGVLAEVLDAAAAWIERQR"
     gene            complement(4980..5741)
                     /locus_tag="AC16CY_08155"
     CDS             complement(4980..5741)
                     /locus_tag="AC16CY_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3353798.1"
                     /translation="MSGWLSSGFGWASSAEGWIALTTLTVLEIVLGIDNIVFISILAG
                     KLPKADRPRARRVGLSLAMLIRIALLLSITWVMSLTAPLFSLVGQEISGRDVILIVGG
                     LFLLGKSTLEIHEKLEGAEGHGSAKVAASFAAVIVQILLLDIVFSLDSVITAVGMAED
                     VAVMILAVVIAVGVMLFSASAISDFVDRHPTVKILALSFLLLIGVSLIGEGMDQHIPK
                     GYIYFAMAFSVFVEMINLRVRAKTEPVHLHQAYKE"
     gene            complement(5752..6261)
                     /locus_tag="AC16CY_08160"
     CDS             complement(5752..6261)
                     /locus_tag="AC16CY_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353799.1"
                     /translation="MAVAPEIPRPSQLWKGLSPERKLQAADAFWQDENAAAEQAEAIV
                     LIAQRIKFRPTSVYALPREKKARHLVGLGAVSDIVAARLLVAYHLRHQRPMMGAFLDA
                     LGVTHEDGLIADEQVQSPPADRLRDAAGSIAASYPPADVTLYLSTLMWQDPDTWAGLA
                     DVPQLSTPA"
     gene            6290..6640
                     /locus_tag="AC16CY_08165"
     CDS             6290..6640
                     /locus_tag="AC16CY_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3353800.1"
                     /translation="MPKLTVEGFAAVDVEQGKRLVLAIEQDAHVDILHACGGNARCTT
                     CRVEFISGEPPRMTKAEQVRMSERGLTGVRLSCQIECNHDMTVRAVSRLTGSGRPDAG
                     STPLPTIQPPPEWI"
     gene            complement(6751..7758)
                     /locus_tag="AC16CY_08170"
     CDS             complement(6751..7758)
                     /locus_tag="AC16CY_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3353801.1"
                     /translation="MSLFSHDLAIDLGTANTCVFARGAGIVLNEPSIVAVNTVNDQIE
                     AVGNDAKEMLGRTPGNITAIRPMKDGVIADFEAAEKMLGHFIKKAHRRKSFVRPRVVI
                     GVPSEITQVERRAVRDSAYRAKVSEVHLIEEAMAAAIGAGLPITEASGNMIVDIGGGT
                     TDIAVISLAGIVYSKSVRVAGNAMDEAITQYVKREHELLIGERTAERIKIELGSAAPL
                     ATRKQMEVKGRHVMEGKPTTVVLHDGQIREALAEPVRLMIQAVRDALERIPPELSADV
                     CDRGIVLSGGGALLQNFDERLRMETGLPVQVAEDPLSAVVLGAGKMLSDFNLLRKISI
                     D"
     gene            complement(7946..>9736)
                     /gene="typA"
                     /locus_tag="AC16CY_08175"
     CDS             complement(7946..>9736)
                     /gene="typA"
                     /locus_tag="AC16CY_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR3353802.1"
                     /translation="RRNVAIIAHVDHGKTTLVDGLLRQSGAFRANQQVADRVMDNTDL
                     ERERGITILAKNTAVHYKDLLINIVDTPGHADFGGEVERTLSMVDGVMLLVDASEGPL
                     PQTRFVLRKALERGLPPIVVLNKIDRADARPAEVLNEVYDLFIDLDATEDQIEFPVLY
                     TNAREGTATADPAKPGDSFEPLFEAIVDHVPPPRGDPGAPLQMLVANLDSSDYLGRIA
                     IGRIFNGAVRLNDSVSVLKLGGAEQQTRVTKLFAFDGLKRVEIDHASAGDIVCLAGIE
                     DITIGETIADLEHRVRIPPIAVDEPTVSMIFGVNTSPRAGRDGQYVTSRNLRDRLEKE
                     LLGNVSLRVEDTGSPEQLSVIGRGELQLSILIEMMRREGYEVQVSRPDIVTKTIDGVR
                     MEPVEELVIDVPEEFQGVVIAQAGTRRGVISRMVNHGSGRVRLEFRIPTRGLIGFRSQ
                     FLTDTKGTGIMNHLFSGWEPWHGAIPARSTGALVADREGVATAYAIFNLQERGEIFVE
                     PGTAVYEGMIVGENSRSSDMDVNVTKEKKQTNMRASTADEAIRLIPPRQLSLEQAIEF
                     INDDELVEITPKSIRLRKRVLAANQRPRKD"
BASE COUNT         1591 a         3270 c         3211 g         1664 t
ORIGIN      
        1 tgacttttca cctgtgacct ttgacccttg acttttcagt tttgagtttt gagttttgag
       61 tttcgagttt cgagttgtga attaccgccg catcatccac atcgacatgg acgccttcta
      121 cgcgtccgtc gaacaacgcg atcgccccga gcttcgcggc cgcccggttg ccgtcggcgg
      181 cgatccggac cgccgcggtg ttgtggccgc cgccagctac gaagcgcgca cgttcggcgt
      241 ccgctccgcg atgtccatgg cgaaagccgt ccgtctctgc cccccgctcc acatcgtcca
      301 tccggacttc gcgaagtatc gcgccgtgtc gcagcaggtc ttcggcatcc tgcggaccgt
      361 gacgccgctg gtcgaaccga tgtcgctgga cgaggcgtac ctggacgtga ccgagaacgc
      421 gtggggcgcg acgctgggga tggacgtggc tcgacggctg aaggaagaga ttcgcgccac
      481 gaccggcctg acggcatcgg ccggcgtcgc gccgaacaag tttctcgcca aaatcgcgtc
      541 gggctggcag aaacccgacg gcctcaccgt cgtcgcgccc gagcgggtcg agtcctttct
      601 gcaggcgctg ccggtcgatg cgctgtgggg cgtgggtccg gtcaccgcga agaagctgcg
      661 tgaccgcggt atcgagcggc tcgtcgacat ccggaccgtc gaacccgagc ggctgcgcga
      721 cacagtggga tcgctggcag actggctgca gcagctggcc aagggcatcg atgaccggcc
      781 cgtcgtgtcg gagtacgagc cgcaatcctc ggggagcgag aacaccttcc ccaccgacct
      841 gaccgacctg ggcgagattc gggtcgagat cgcggacatg gcgcgcagcg ccgctgcgtg
      901 gctcgagaag cgcgagctgt acgcccgcac cgtgaccatc aaggtgcgct acagcgactt
      961 caccactatc acgcgcagcc acacggaagc cgcgacgcgg gacgacggcc gcctggccgc
     1021 gcgcgccgtc gcgcttctgg aaaagaccga cgccgggcag cgccccgtcc ggctgctggg
     1081 cgtcagcgtc cacaatctgt gcgacgcgct cgccccgccg aagcccgcaa ccggcaggct
     1141 gcctttcgag tcttgagtct tgagttctga gttttgagtc ttgagttacg ggcgcagcac
     1201 ccgcaccccc gtcacttcga tccgcgtcac gggtgtttcg ccgtcgagcg gtactgcctc
     1261 gatcttgtcg agcacgtcga gcccgccgac gacgcggccg aagacggtgt actggccgtc
     1321 gagcgacgtc gcacgggcca acacgatgaa gaaagagctc gtcgcgctgg ccggatcgct
     1381 gagacgcgcc atcgagacga tcccgcggtc gtgcggcgtc gcgttgaact ccggctgcag
     1441 cgtccggacg tagccctgct gccgctcgcc gagcggcgcc cgccgcgtgg gaagatggcc
     1501 cccctgggcc acgaagccgc ggaccacacg gtgaatggcg gtgccgtcgt acacgcccga
     1561 cgcggccagc cgcaggaact ggcggacgtg gttcggcgcg cgatcggcaa agagctcgag
     1621 cgtgatgtct cccatcgagg tcgcgatcac ggctcgagac gatgacagct cctcgacgct
     1681 tgccgacgcg aacggctccg gctctggcgc cggcttgtcc cggatcgtga ccttccggat
     1741 ctcgatgcgg gccgccggca cgctgttgct ggctggcgtc gtggagatcg cctgcgccac
     1801 gttgatgcct tcagcgacgc gcgcaaacac cgtgtactgt ccgtcgagcg acggctgatc
     1861 ggtgacgacg atgaagaact gcgagccggc gctgtcgcgg tttcccggca cgagcacggc
     1921 cgagacggcc ccgcgcgtgt gtttctccgc attcggctcg aacctgagcc tgccgaggcc
     1981 gcccgtgccg tacttggctg actggcccgg atccttcgac agcggatcgc cgccctggac
     2041 gatacccatc gagatcacgc gatgaaatgt cgtcccgtcg tacgcgcccg cacgcgcccg
     2101 atcgatgaaa tacgccacgt ggttgggcgc cgactcgggc agcagatcga gcacgatggc
     2161 gccctcgctc gtgtcgagca ccgcctgctt gccgcgcatc tcgtcgagcg gcagctgcgc
     2221 agtaaagaac gtgtcgcgcg gagggggagc aggacgctga gggcggacag cctgcggatc
     2281 ggccagcagg accagcaaca tcagcggcag catgccgccg attatctgtt atattttctg
     2341 caagttgagc gcttcagcca cgccaacgcg cagcatcctg cagtacgagg agttctacgg
     2401 cttcgtacag tcgcctttca cgctggctcc cgacccgcgc tttctctatc gcagctcctg
     2461 ccacgaagac gcgctccggc tgctactgca atcggtcggc cggcgcgagg ggatcgccgt
     2521 cctgagcgga gacatcggca ctggcaagac aactgtctgc cggaccctcc tcgaacagct
     2581 ggacatgacg cggttcacgt cgcttctgtt gaacccgttc ctgtccgtgg aggagctgct
     2641 gcgcgagatc ctgctcgatt tcggcgtggt ctcgcgcgaa gccgtgcgga gcgggcgggt
     2701 cacctcggcg agcaagcacg agctcgtgag cgcgctgcac gagttcctga tctcgctcgt
     2761 gccgatcggt gcgagcggcg tgctcgtgat agacgaggcg cagcacctct cgacgcaggt
     2821 cctcgaggag attcgcgtcc tctcgaacct ggagaccaac cagaacaagc tgctgcagat
     2881 catcctcgtc ggccagctca acctcctgga gatcctgaag cagccggaac aacgccagct
     2941 cgaccagcgc atcacggcgc gtgcagtcat caggccgctc gaccgccccg aggtcgaggc
     3001 gtatgtggcg caccggctgg gcgtggccaa ggggtcggcg caggtgcggt tcgacgccgg
     3061 agcgctcgat gcggtgcagc tggcaagcgg cggcgtgcca cgcctgatca acctggtctg
     3121 cgatcgcacg ctcatgctcg gcgcgcagct cgatctccgc acgatgtcgt cggccgtcgt
     3181 cggcgcggcg gccgagagcc tggcgctgcc cgcggacaag ccggcgtccc cgcgctggtg
     3241 gcagcgcctg ttccgccgat cgtcatcctc gctttgactt gaggtgctgt ctgagcgcct
     3301 tggcgccaat cttcccgcgc cttttctgct cgagctgggc gcgctcgtcc tgttgccgtg
     3361 ccagatgtga cagttcgtcc tggagtttca ggtagctgta caggcgctcc tgcggaatgc
     3421 gcccatcgtc cactgccacc ttgaccgcgc agcgcggctc gcctcgatgc cggcagttgc
     3481 cgaagtggca cgaggcggca agcgcctcga tgtcgtcgaa ggtctcggtg agcgcctcgc
     3541 tgacgtccca caactgcagc tcgcgcatcc ccggcgtgtc gatgatgaac ccgccgtgcg
     3601 gcaggaagac gagctcgcga tgggacgtgg catggcggcc cgtcgagtcc gactcccgca
     3661 cttctttcgt cttctgcacg tcccggccga cgagccggtt gatgatggtg ctcttgccga
     3721 cgcctgagga gccgagcagc gcccccgttc ggcccgacgg caggtagtcc acgaggcgat
     3781 cgactccgag attcagcttc ggactgacca cgtgcaccgg aacgcctccg ccaatcgcgg
     3841 agacttcgcc gacccggccc tcgacatcgg cgcacaggtc cggcttggtc aacaacacga
     3901 cgggcgaggc gccgctttcg cgggcgagca gcagataccg ctcgagcctg cggaggctga
     3961 aatccgcgtc gagagccatc acgaggaaca cgacgtccac gttggcggca acgacctgct
     4021 cgccggtaac cttgccggcc atccggcgcg agaaccagct gcgccgcggc agcacgccga
     4081 cgatggcgcc gcgatcctcg tcggggcgct tgcggatcgc cacccagtcc cccacggcag
     4141 gcagctctcc acggctcgcg gcccggtgct tgaggcggcc cgccagcacg gcctccgttt
     4201 ccccctgcgg gccccaggca cggtagatgt agttgaactc gatgaggatg cggcccggct
     4261 gcatgtcctg tttgccggcc caggcttcat acgcgcgcgc gagggcgtcg tcccagccga
     4321 gctgttccag tccggtcatc gctgacgctc gatccatgcg gccgccgcgt cgagcacctc
     4381 ggccagcacg ccttcccgcc ggccgcgcac cgtcagcgag tggtcaccac ccggaatcgg
     4441 gagcatctcc gcgcccgcgg gcatcgtgtc gagcacgggg gccagctccg ccggcgtgcc
     4501 gaacgtgtcg cgctccccct ggacgacaag cacggggacc ttgacggaag gcaggtgatc
     4561 gactcgcaat cgttcgggct tcccgggagg atgcagcggg tagccgaaca cgatgatgcc
     4621 ccggagacgg tccacaccag cagcgcccag gtgcgtcgcc atccggccgc ccatcgactt
     4681 gccgccgatg aacgtcctgt cggcgcggct ccaccggcgc gccgcgtcca cgacgtcgag
     4741 gaatgccttt tccagcaccg gggcgcgatc ggggacgtgg cggcgcgccc gcatgtaggg
     4801 aaaatcgaag gtcacggcgt cgatgccccg ggccgcaaac ccgcccgcga cggcagtcat
     4861 gaatggatgc cgctgccccg cgcctgcgcc atgccccagc acgatcagcg cgcgcgtcgc
     4921 gccttcggcc cggtacccca ccgcgccgag atccgtcaga tccattgcac gagcctggct
     4981 tactccttgt acgcctggtg caggtggacc ggctcggtct tcgcgcgcac gcggaggttg
     5041 atcatctcga cgaagaccga gaaagccatc gcgaagtaga tgtagccctt tggaatgtgc
     5101 tggtccatgc cctcgccgat gagcgacacg ccaatcagca gcaggaagct cagggcgagg
     5161 atcttcacgg tgggatgccg atccacgaaa tcgctgatcg cgctggcgga gaacagcatg
     5221 acgccgacgg cgatgacgac cgccaggatc atcacggcga cgtcttccgc catgccgacc
     5281 gccgtgatca ccgaatcgag cgagaagacg atgtcgagga gcaggatctg cacgatcacc
     5341 gccgcgaacg aggcggccac tttcgcggag ccgtgccctt cggcgccctc gagcttttcg
     5401 tggatctcga gcgtgctctt cccgagcagg aacaggccgc cgacgatgag aatgacgtcc
     5461 cgccccgaga tctcctggcc caccagactg aacaagggcg cagtgaggct catgacccag
     5521 gtgatggaca acaggagcgc gatccggatc agcatcgcga gcgagagacc gacgcggcgg
     5581 gcccgcggcc ggtcagcctt cgggagcttg ccggccagga tcgagatgaa gacgatgttg
     5641 tcgatgccga ggacgatctc cagcacggtc agcgtcgtca gcgcaatcca gccctccgcg
     5701 ctcgacgccc agccgaatcc cgaggacagc cagccgctca tcgcgctgtt tctaggcggg
     5761 cgtcgagagc tgcggaacgt ccgcgagccc ggcccacgtg tcggggtcct gccacatgag
     5821 ggtcgagaga tagagagtca cgtcggccgg cgggtaggac gcggcaatgc tcccggcggc
     5881 gtcacgcagg cgatcggccg gcggcgactg cacctgctcg tcggcgatca gaccgtcctc
     5941 atgcgtgacg ccgagggcat cgagaaaagc tcccatcatc ggacgctgat ggcggaggtg
     6001 atatgccacg agcaggcgcg cagcaacgat gtccgacacg gcgccgaggc ccacgagatg
     6061 gcgggctttc ttctcgcgcg ggagcgcgta cacgctggtg ggccggaact tgatccgctg
     6121 cgcgatgagc acgatcgctt cagcctgctc ggcagcggcg ttctcgtcct gccagaacgc
     6181 gtcggctgcc tgcagcttgc gttcgggcga gaggcctttc cacaactgcg acggtctcgg
     6241 gatctcggga gcgacggcca tggagtctcg atacgttaac atagcgcgca tgccgaagct
     6301 gaccgtggaa gggttcgcag ccgtcgatgt cgagcagggc aagcgcctgg tccttgcgat
     6361 cgagcaggac gcgcacgtgg acatcctgca tgcgtgcgga ggcaacgcgc gctgcacgac
     6421 gtgccgcgtc gagttcatca gcggcgagcc gccgcggatg acgaaggcgg agcaggtgag
     6481 gatgagcgag agggggctga caggcgtccg gctctcctgc cagatcgaat gcaatcacga
     6541 catgacggtc agggccgtga gccggttgac cggcagcggg cggcccgacg cggggtccac
     6601 gccgttgccc acgattcagc cgccgcccga gtggatttga ctgacgtagt gtccggcttc
     6661 agccggacca gtggattcga ccgtcgtagt gtccggcttt agctggacca gcggattcga
     6721 cggtcgtagt gtccggcttt agccggaccg tcaatcgatc gagatcttcc gaagcagatt
     6781 gaagtccgac agcatcttgc cggcgccgag cacgacggcc gagagcgggt cctcggcgac
     6841 ctgcacgggc aggccggttt ccatgcgcag gcgctcgtcg aaattctgca ggagcgcgcc
     6901 gccgccgctc aacacgatgc cgcggtcgca cacgtcggcc gacagctccg gcggaatccg
     6961 ctcgagcgcg tcgcgcacgg cctgaatcat caggcgcacc ggctcggcca gcgcttccct
     7021 gatctggccg tcgtggagca ccaccgtcgt cggcttgcct tccatgacgt ggcggccctt
     7081 gacctccatc tgcttccgcg tcgcgagcgg cgcggccgac ccgagctcga tcttgatgcg
     7141 ctcggccgtg cgctcgccga tgagcagctc gtgctcgcgc ttgacgtact gggtgatcgc
     7201 ttcgtccatg gcgttaccgg ccacgcgcac cgacttgctg tagacgatgc cggcgagcga
     7261 gatgacggcg atgtccgtcg taccgccgcc gatgtccacg atcatgttcc ccgaggcttc
     7321 ggtaatgggc agcccggcgc cgatggcagc cgccatcgcc tcctcgatga ggtgcacctc
     7381 gctgaccttg gcacgatagg cgctgtccct gacggcgcga cgctccacct gggtgatctc
     7441 ggatggcacg ccgatgacga cgcgcggccg cacgaagctc ttccgcctgt gggccttctt
     7501 gatgaaatga ccgagcatct tctcggccgc ctcgaagtcg gcgatgacgc cgtccttcat
     7561 cgggcggatg gcggtgatgt tgcctggcgt ccggccgagc atctccttgg cgtcgttgcc
     7621 aacggcctcg atctgatcgt tcacggtgtt gaccgcaacg atggagggtt cgttcagcac
     7681 gattccggcg ccgcgcgcga agacgcaggt gttggcagtg ccaagatcga tggccaggtc
     7741 gtgggaaaat agggacattg ccccaaggaa catcagcggc ctccagttag ctcgagttgc
     7801 ggccgtccgg ggcaaggagt gtacccgcag agttgggtag gtgcccccgc ttgaattgcg
     7861 cgggaattca gcgaactgcg aagaatccgt cgtcagagcc gtggacggat ttgggccatg
     7921 gatcgcaatt acgtgatcga cgatttcaat ctttgcgtgg ccgctggttc gcggcgagca
     7981 cccgtttgcg cagcctgatg gacttcggcg tgatctcgac gagctcgtcg tcgttgatga
     8041 actcgatggc ctgctcgagg ctcagctggc gcggcgggat cagccggatc gcttcgtcgg
     8101 cggtcgaggc gcgcatgttg gtctgtttct tctccttcgt gacgttcaca tccatgtcgc
     8161 tcgaacggga attctccccg acgatcatcc cttcgtagac cgccgtcccc ggctcgacga
     8221 agatctcgcc ccgctcctgc aggttgaaaa tcgcgtacgc cgtggccacg ccctcgcgat
     8281 cggccacgag tgcgccggtc gaacgggccg gaatcgcgcc gtgccacggc tcccacccgg
     8341 aaaacaggtg gttcatgatg ccagtgccct tcgtgtcggt caggaactgg gaacggaatc
     8401 cgatcagtcc gcgcgtcgga atccggaact ccagccggac gcggccgctg ccgtggttga
     8461 ccatcctgga gatgacgccc cgcctggtgc ccgcctgcgc gatcacgacg ccttgaaact
     8521 cttccggcac gtcgatgacc agctcttcga ccggttccat ccgcacgcca tcgatcgtct
     8581 tggtcacgat atccgggcgc gacacctgga cctcgtaccc ttcgcggcgc atcatctcga
     8641 tcagaatcga gagctgcagc tcccctcgcc cgatcacgct caactgctcc ggggagccgg
     8701 tgtcctcgac acgcagcgag acgttgccca gcaattcctt ctcgagacgg tcgcgaagat
     8761 tccgcgacgt cacgtactgg ccatccctgc cggccctcgg agacgtgttg accccgaaga
     8821 tcatcgacac cgtcggttcg tccacggcaa tcggcggaat cctgacgcgg tgctcgaggt
     8881 cggcgatcgt ctcgccgatc gtgatgtcct cgatcccggc aaggcagacg atgtcgccgg
     8941 cgctcgcgtg gtcgatttcg acgcgtttca acccgtcgaa tgcgaacagc ttggtcacgc
     9001 gggtctgctg ttcggcgccc cccagcttga gcaccgacac ggaatcgttc agcctcacgg
     9061 ccccgttgaa gatgcgcccg atggcgatcc ggccgagata gtcgctcgag tccaggttgg
     9121 cgacgagcat ctgcaggggc gcgccggggt cgcctcgcgg aggaggcacg tggtcgacaa
     9181 tcgcttcgaa caggggctcg aagctgtctc ccggcttggc gggatctgcc gtcgcggtgc
     9241 cctcgcgcgc gttggtgtag aggacgggga actcgatctg atcctcggtg gcgtcgaggt
     9301 cgatgaagag gtcgtacacc tcgttgagga cttcggccgg ccgcgcgtcc gcccggtcga
     9361 tcttgttcag gaccacaatc ggggggagac cccgctcgag cgccttccgg agcacgaagc
     9421 gggtctgagg cagcgggcct tccgaggcgt cgacgagcag catcacgcca tcgaccatcg
     9481 acagcgtgcg ctcgacttcc ccgccgaagt cggcatggcc cggcgtgtcc acgatgttga
     9541 tcaggaggtc cttgtaatga accgcggtgt tcttcgccag gatcgtgatg ccccgctcgc
     9601 gctcgaggtc ggtgttgtcc atgacgcggt cggccacctg ctgattggcg cggaacgcgc
     9661 cgctctgccg cagcaggccg tccaccagcg tcgtcttgcc gtgatcgacg tgggcgatga
     9721 tggccacgtt gcggcg
//
LOCUS       DBNGSH010000348         8217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3085576, whole genome shotgun sequence.
ACCESSION   DBNGSH010000348 DBNGSH010000000
VERSION     DBNGSH010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3085576"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="AC16CY_08180"
     CDS             complement(<1..251)
                     /locus_tag="AC16CY_08180"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3353803.1"
                     /translation="MKHLTLANQLTILRIMLIPAFVLLVVYGYLGAALLVFGIAGLTD
                     ALDGLIARVTRQRTSLGAWLDPMADKLLLVSTFVVLTVPA"
     gene            complement(248..1645)
                     /locus_tag="AC16CY_08185"
     CDS             complement(248..1645)
                     /locus_tag="AC16CY_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3353804.1"
                     /translation="MRVESGRGARVRSAVRASAALLLCALAAACAPKAPPVAPGVLAH
                     PEFVYPLVPLALAGTAGASRIDVGWQYLQAGDVRNAELEFNVALKLGPRLYPARAGQG
                     YLALARRDYDRALTAFDAALKDDGKYVPALVGRGQALLALDRVAQALTAFEAALAVDP
                     SLVDVQRRVEVLRFRSLQDLIDAARQATSDGRLADARLAYDRALAASPDSAFLHRELG
                     LLERRAGNTDRALTELRRAVELDAADAASLTAIGDVLDAEGDLAGAEAAYRQAAAIDP
                     SQVLTAKIADVSKRALGASLPPEFTAALTAPQITRGDLAALIGVRLEELVRRAPVLQV
                     VMTDTQGHWAARWIADTASAGIIEPFENHTFQPGTRVRRGDLATVASRLVAMIAAADP
                     AVRARVAQRPAIADMSPTHLQYDAVVSVVAAGVMPLADGGRFQVSNQVSGAEAVDAID
                     RVRALAATMLGTSRP"
     gene            complement(1635..2918)
                     /gene="hflX"
                     /locus_tag="AC16CY_08190"
     CDS             complement(1635..2918)
                     /gene="hflX"
                     /locus_tag="AC16CY_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR3353805.1"
                     /translation="MSSPRERAALVGLVTGAARRADADQSLDELAGLADAAGADVALR
                     VLQERAKPDPATFIGSGKVTSLTFACDEADVDLVIFDNELTPAQLGQLEKRLARKVVD
                     RTQLILDIFARRARTREGKWQVELAQLKYLLPRLAGSGTALSRLGGGIGTRGPGETKL
                     ETDRRRIRMRIQAIQRDIDQMRQRRSQLRERRQKRSVPTVALVGYTNAGKTTLFNRMT
                     NESAVASDALFVTLDPLVRRVKLPDRRELLVSDTVGFIDRLPHALVAAFRGTLEEVAE
                     ADLALHVIDASSRERDRHVAAVLRVLEEVDAAAVPRIDVYNKVDVIGTDASRRLQDAH
                     PAASLVSARTGEGVADLMRMVAARLALDTRRVTITFDNSSEFDRQQVSRLYRVARVIS
                     HVNTNGRVVIEADVPRRFIERLTSSADSGDASDAR"
     gene            2942..3082
                     /locus_tag="AC16CY_08195"
     CDS             2942..3082
                     /locus_tag="AC16CY_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353806.1"
                     /translation="MKRTSAKAKRRPSRPRVPLPRQTGGIHEDKTKRPWRRRKHKHEI
                     DD"
     gene            complement(3183..4394)
                     /locus_tag="AC16CY_08200"
     CDS             complement(3183..4394)
                     /locus_tag="AC16CY_08200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3353807.1"
                     /translation="MDTWRRNQVAVTAASFVGFTGFTLVMPFLASYFQQLGVTDRGDA
                     ALWTGATLGVTPAVVAICAPLWGRVGDRFGNKLLLQRSLISCVVVMWLMAYAAHPWQL
                     FGLRALQGLAAGYGPLTISMAALSAPRDGMARAIGMVQTAQRIGPAVGPLIGGLLAAA
                     VGLRQAFLVSAAVYACAFVMVTVLYSEPPRAARRHEEGQRVSFGTVLAFESFLLLMLV
                     IFGLQLVDRSFGPVLLLHARDLGYGPGDAAVLVGALFSVLAFAGAFGNQLAAALLKRG
                     TTRAVIAGSVLVAAAALSVFALATAVWLMTGAIALFGAAVGTAMTATFTAAGSVVPRH
                     AHGVGFGFLSSASLTGSAVSPVLSGLVADQSITVVFLSGAALLLLLVLLVRRLMAERH
                     PTVESPSVDES"
     gene            complement(4402..5433)
                     /gene="glpX"
                     /locus_tag="AC16CY_08205"
     CDS             complement(4402..5433)
                     /gene="glpX"
                     /locus_tag="AC16CY_08205"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HIR3353808.1"
                     /translation="MTTTSSTHSEVDRSALFDSDLSLDFLRVVEQAAIACAHTMGQGD
                     RHKSDQVAVEAMRREMDSVPIDGTIVIGEGERDEAPMLYIGEKVGMARGIAGPYPQVD
                     IAVDPLEGTNLCATGAANALAVLAASEGGGLLHAPDIYMEKLVVGPSARDVVDLDAPV
                     RFNLRAIAQANSRKVEDLVVIVLDRPRHTKLIDEIRATGARIRLIGDGDLSAGIAAAV
                     VGSGVHAVMGTGGAPEGVLTAAAMRCLGGEIYARLVVSTPEHERRCKAMGIHDVTRTY
                     RSKDLASGTNIVFAATGVTDGTLMKGVRFFGDGIRTTSLVMQTHPHRIRFIDSIHVHA
                     KGPTAKIRF"
     gene            complement(5430..6635)
                     /locus_tag="AC16CY_08210"
     CDS             complement(5430..6635)
                     /locus_tag="AC16CY_08210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003395828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3353809.1"
                     /translation="MLNFDLSDEQRLLEQSVREWAGREVAPRIRELDRSHRFDRGLLP
                     QMAELGLLGISVPAEYGGAGMDYICLGLASEELEYVDTSLRVILSVHVGLNSLTLLSW
                     GTEDQKQRYLVPQAQGRKIATYGLTEPSAGSDARGIQTVAIKRSDRYELTGEKTWISL
                     ADVADNFLVFAWTDLDKKKQRDPSGMSAFIVERGFAGFSSGTLTEKWGILAGNTGYFK
                     MDNVAVPEENLVGREGEGFKIAMFALDQGRYTVAAGATGLIRACRDASVAYATERKTF
                     GVEIGSHQLVKELIAQMESDYQASRLLWHRAGWLKNQGRRNTRETGLAKWFSTVASER
                     AAGDAVQIHGANGYSDDYPVGRFYRNCKGAVIYEGTREIHKLMQADYLLGYRTDRPTR
                     CDLPAWEAR"
     gene            complement(6635..7321)
                     /locus_tag="AC16CY_08215"
     CDS             complement(6635..7321)
                     /locus_tag="AC16CY_08215"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3353810.1"
                     /translation="MALSDLFSRRRREHADSDTGPSDSPVYPTKVLARFIAGLSTKPQ
                     PVLLDLGPAVGANVNFFGEELGCKIIFAELSKDIDRHVREGALDQLPSFLAKRFPQDS
                     SSIDGILCWDLFDYLDKKSAKPLAEQLTRILRPEGVLLVFFSTAEPRPGTRPEYTKHV
                     VINRTHLQHRPYAAARSRQRPLPNRDIQRLFEPLRVVESFLLQTNLREVLFRKTSETS
                     SPPTAITRPA"
     gene            complement(7388..7939)
                     /locus_tag="AC16CY_08220"
     CDS             complement(7388..7939)
                     /locus_tag="AC16CY_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR3353811.1"
                     /translation="MWKRDEAVKPTSGQPAAPAPIASTQPASAPVAAQPDVRRIERDM
                     VNIGKSVVIKGELNGSEDLTVEGHVEGRIELKDHVLTIGPNGKIKAQVFAKAVIVLGE
                     VNGNVTASEKVDIRDGGSVDGDIIAPRVAIAEGAHFRGSVDMQRKGAAQPAAQPQKAV
                     STPPEKASTPPEKAQAQPQAAAH"
BASE COUNT         1360 a         2885 c         2697 g         1275 t
ORIGIN      
        1 gccggaaccg tcagcaccac gaaggtcgac acgagcagga gcttgtcggc catcgggtcg
       61 agccacgcgc ccaggctcgt gcgctggcgg gtcacccgcg caatgaggcc gtcgagcgca
      121 tcggtcaggc cggcaatccc gaacaccagg agcgccgcgc cgaggtagcc gtagacgacc
      181 agcagcacga acgccgggat cagcatgatt cgcaggatcg tcagctggtt ggcgagcgtg
      241 aggtgcttca cgggcgtgac gtgccgagca tcgtcgccgc cagtgctctc acgcgatcga
      301 tggcgtcaac cgcctcggcg ccggagacct gattcgacac ctggaaccgc ccgccgtcgg
      361 cgagcggcat cacgccggcg gcgacgaccg acacgacggc gtcgtactgc aggtgcgtcg
      421 ggctcatgtc ggcgatcgcg ggacgctggg ccacgcgcgc gcgcacggcg ggatcggccg
      481 ccgcgatcat ggccaccagc cggctggcca ccgtcgccag atcgccgcgc cgcacgcggg
      541 tgcccggctg aaacgtgtgg ttctcgaacg gttcgatgat gccggcgctg gcggtgtcgg
      601 cgatccagcg ggcggcccag tgcccctgag tgtccgtcat gacgacctgg aggaccggcg
      661 cccgccgcac cagttcttcc agccgcacgc cgatgagcgc cgcgaggtcg ccgcgcgtga
      721 tctgcggcgc ggtcaacgcc gcggtgaact cgggcggcag gcttgccccg agcgcccgct
      781 tggacacgtc agcgatcttc gcggtcagga cctgactggg gtcgatggcg gccgcttgac
      841 gataggcagc ctcggcgccc gcaaggtcgc cttccgcgtc gagcacgtcc ccgatcgccg
      901 taagagaggc ggcgtcggcc gcgtccagct cgacggcccg tcgcagctcg gtgagggcac
      961 ggtccgtgtt gccagcgcgc cgctcgagca ggcccagctc gcgatgcagg aacgcgctgt
     1021 cgggcgacgc ggcgagcgcc ctgtcgtagg cgagccgcgc gtcggcaaga cgcccatcgg
     1081 acgtggcctg acgtgcggcg tcgatgagat cctgaaggct ccggaagcgc agaacctcga
     1141 cgcgccgctg cacgtccacg agcgagggat ccacggccag cgcggcttcg aacgccgtca
     1201 gcgcctgcgc gacccggtcg agagccagca gcgcctgccc gcgcccgacg agcgccggta
     1261 cgtacttgcc gtcgtccttg agcgcagcgt cgaaggccgt cagggcacgg tcgtagtcgc
     1321 gtctagccag cgccaggtag ccctgcccgg cgcgggccgg gtagagacgc ggcccaagct
     1381 tcaaggccac attgaactcg agttcggcgt tccgcacatc gcccgcctgc aggtactgcc
     1441 acccaacatc gatccgcgac gcgcccgccg tcccggctag cgctagcggc accaacgggt
     1501 agacgaattc cgggtgcgcg agcacgcccg gcgccaccgg cggcgccttg ggcgcgcacg
     1561 ccgcggcgag cgcgcacaac agcaacgccg cagaggcccg cacggcggac cggactcgcg
     1621 cccctctgcc gctctcaacg cgcatcggaa gcatcccccg aatcggcgct cgacgtcagc
     1681 cgctcgatga accggcgcgg cacgtccgcc tcgatgacga cacgcccgtt ggtgttcacg
     1741 tggctgatga cccgcgccac acgatacagc cgcgacacct gctgccgatc gaactcgctg
     1801 ctgttgtcga acgtgatcgt gacacgcctg gtgtcgagcg cgagccgtgc ggccaccatc
     1861 cgcatcaggt cggcgactcc ctccccggtt cgggccgaga ccagcgacgc cgccgggtgg
     1921 gcgtcctgaa gacgccggct cgcgtccgtg ccgatgacgt ccaccttgtt gtacacgtcg
     1981 atccgcggaa cagcggcggc gtccacctct tcgagcacgc ggagtacggc cgccacgtgc
     2041 cgatcgcgct cccggctgga ggcatcgatg acatgcagcg cgagatcggc ctccgcgacc
     2101 tcctcgagcg tacctcgaaa tgcggcgacg agcgcgtgcg gaagccgatc gatgaagccg
     2161 acggtgtccg agacgagcag ctcacgccga tcgggcagct tgacgcggcg gacgagcgga
     2221 tcgagcgtca cgaacagggc atcggaggcc accgcgctct cgttcgtcat ccggttgaac
     2281 agggtcgtct tgcccgcgtt ggtgtagccg acgagcgcga cggtcgggac cgaccgcttc
     2341 tgccggcgtt cgcgcagctg cgagcggcgc tgccgcatct ggtcgatgtc ccgttgaatg
     2401 gcttggatac gcatccgaat acgacgccga tcggtttcca gtttcgtttc gcctggaccg
     2461 cgcgtcccga taccgccgcc gagacgcgag agcgccgtcc cgcttccggc cagccgcggc
     2521 agcagatact tcagctgcgc gagttccact tgccacttgc cttcgcgcgt ccgcgcgcgg
     2581 cgggcgaaga tgtcgaggat cagctgcgtg cggtccacga ccttgcgcgc cagccgcttc
     2641 tccagctggc cgagctgcgc cggcgtgagc tcgttgtcga agatgacgag gtcgacgtcg
     2701 gcttcatcgc acgcgaaggt cagtgacgtc accttccccg agccgatgaa cgtcgcggga
     2761 tccggcttcg cccgctcctg cagcacgcgc aacgccacgt cggcgccggc ggcatcggcc
     2821 agccccgcga gctcgtcgag cgactgatcg gcatcggccc ggcgagccgc gccagtcaca
     2881 aggcctacga gcgccgcccg ttcacgcgga gacgacatac ctgtagctgg tagaattcca
     2941 catgaagcgc acttcggcaa aggccaagcg gcgaccgtcc aggccgcggg tcccgctgcc
     3001 ccgtcagacg ggcggcatcc acgaggacaa gaccaagcgc ccgtggcggc gccgcaagca
     3061 caagcacgag atcgacgact gatcccaaag gttacctgaa tcgagcatga accccgatca
     3121 cgaactccga aactgaacac gaaccgagaa gtgagaagtg tgaacagctg atccgatcca
     3181 ggctacgact cgtccaccga cggggactcg actgtcggat gtcgctcggc catgagacgt
     3241 cgaacgagca ggacgagcag caacagcagc gccgcgcctg acagaaacac gaccgtgatg
     3301 ctctggtcgg ccaccaggcc gctcagcacc ggactcaccg ccgacccggt gagagaggcg
     3361 ctcgacagga aaccgaaccc gacgccgtgc gcgtgccggg gcacgaccga tcctgccgcg
     3421 gtgaaggtcg ccgtcatcgc cgtgcccact gcggcgccga acagcgcgat cgcaccggtc
     3481 atcagccaga ctgccgtggc gagcgcgaac acgctcaagg ccgcggcggc gacgagcacc
     3541 gatccggcga tgacggcacg cgtcgtgccc cgtttcagca gcgccgccgc gagctgattc
     3601 ccgaacgcgc cggcaaaggc gagcacggaa aacagcgctc cgaccagaac cgccgcgtcg
     3661 ccggggccgt accccagatc gcgcgcgtga aggagcagca caggaccgaa gctgcgatcg
     3721 accagctgca acccgaagat gacgagcatc agcagcagaa agctctcgaa cgccaggacg
     3781 gtcccgaagc tgacgcgttg cccctcctcg tggcggcgcg ccgcgcgcgg cggctccgag
     3841 tacaggacgg tcaccatcac gaaggcgcaa gcatagacgg ccgccgacac gaggaacgcc
     3901 tgcctcaacc cgacggccgc cgcgagcagg ccgccgatca gcggtccgac cgccggcccg
     3961 atgcgctgtg ccgtctgcac catgccgatg gcgcgggcca tcccgtcccg gggcgccgag
     4021 agcgccgcca tcgaaatggt cagtggaccg taccccgcgg ccaggccctg aagggcgcga
     4081 agcccgaaca gctgccaggg atgcgcggcg tacgccatca gccacatgac tacgacgcaa
     4141 ctgatcaggg atcgctgcag gagcagcttg ttgccgaatc gatcgccgac ccgcccccag
     4201 agcggcgcgc agatggccac caccgccgga gtgacgccga gcgtcgcgcc cgtccacagc
     4261 gcggcatcgc cgcgatcggt gacgccgagc tgttgaaagt agctcgccag gaacggcatc
     4321 accagcgtga agccggtgaa gccgacgaag gacgccgcgg tcaccgcgac ctgattgcgc
     4381 cgccaggtgt ccacgacgcg cctagaatct aatcttggcc gtgggcccct tggcgtgcac
     4441 gtggatgctg tcgatgaagc ggatccggtg aggatgcgtc tgcatgacga gcgacgtcgt
     4501 ccggatgccg tctccgaaga accgtacgcc cttcatcaac gtgccgtcgg tcacgccggt
     4561 cgccgcgaac acgatgttcg tgccggaggc caggtccttg gatcggtacg tccgggtgac
     4621 atcgtggatg cccatggcct tgcaccgccg ctcgtgctcg ggcgtgctca cgaccagccg
     4681 cgcatagatt tcgccgccca ggcatctcat cgccgcggcc gtcaacacgc cttcgggcgc
     4741 cccaccggtg cccatcaccg cgtgcacgcc gctgccgacg acggccgccg cgatgccggc
     4801 cgacaggtcg ccgtctccga tcaatcggat ccgcgcgccg gtcgcccgaa tctcgtcgat
     4861 cagcttcgtg tgccgcggcc gatcgaggac gatgacgacg aggtcctcga ccttgcggct
     4921 gttcgcctgg gcgatcgcgc gcaggttgaa ccggacgggc gcgtcgaggt ccacgacgtc
     4981 gcgcgcgctc ggcccgacga cgagcttttc catgtaaatg tcgggcgcgt gcagcagccc
     5041 gccgccctcg gacgccgcca gcactgccag cgcgttggcc gcgccggtgg cgcacaggtt
     5101 cgtgccctcg agcggatcga ccgcgatgtc cacctgcggg tacggcccgg cgatgccgcg
     5161 agccatgccg accttctcgc cgatgtacag catcggcgct tcgtcgcgtt cgccctcgcc
     5221 gatgacgatc gtgccgtcga tcggcaccga gtccatctcg cgccgcatgg cttcgacggc
     5281 gacctggtcg gacttgtggc ggtcgccctg gcccatcgtg tgcgcgcacg cgatcgccgc
     5341 ctgctccacg acgcggagga agtcgagcga gaggtcggaa tcgaagagcg ccgaacgatc
     5401 gacttcggag tgagtcgagc tcgtggtggt catcgtgcct cccacgccgg aaggtcgcag
     5461 cgtgtcggtc tatccgtacg atacccgagc aggtagtcgg cctgcatcaa tttgtggatc
     5521 tctctcgtgc cctcgtagat gacggcgccc ttgcagttgc gatagaaccg gccgacggga
     5581 tagtcgtcgg aatagccgtt ggccccgtga atctggacgg catcgccggc cgcccgctcc
     5641 gaggccaccg tcgaaaacca cttcgccagg ccggtctccc gcgtgttgcg gcgcccctgg
     5701 ttcttgagcc agcccgcacg gtgccacagg agcctcgacg cctggtaatc cgattccatc
     5761 tgggcgatca gctccttcac gagctgatgt gaaccgatct ccacgccgaa cgtcttgcgc
     5821 tcggtcgcgt aggcgacgct cgcatcacgg cacgcgcgaa tcaatccggt ggcgcccgcc
     5881 gccacggtgt agcgtccctg atcgagcgcg aacatcgcga tcttgaagcc ctcgccctcg
     5941 cggcccacca ggttttcctc tggaaccgcc acgttgtcca tcttgaagta gccggtgttg
     6001 ccggcgagaa tcccccactt ctccgtcagc gtgccgctcg aaaaccccgc aaagccgcgc
     6061 tcgacgatga acgcgctcat gccggagggg tcgcgctgct tcttcttgtc gaggtccgtc
     6121 cacgcgaaca ccaggaagtt gtcggccacg tccgccagcg agatccaggt tttctcgcct
     6181 gtcagctcgt accgatccga tcgcttgatc gccacggtct ggatcccgcg ggcgtcgctg
     6241 ccggccgagg gctccgtcag gccgtaggtg gcgatcttcc tgccctgcgc ctgcggaacc
     6301 aggtatcgct gcttctgatc ctccgtgccc cacgacagca gcgtcagaga gttcagcccg
     6361 acgtggaccg acaggatcac ccggagagac gtgtctacgt actcgagctc ctcgctggcg
     6421 aggccgaggc agatgtagtc catccccgcg ccgccgtact cggccgggac cgaaatcccc
     6481 agcagcccga gctccgccat ctgcggaaga agcccgcggt cgaaccggtg gcttcgatcg
     6541 agctcacgta tgcgcggtgc gacctcgcgg ccggcccatt cccgaaccga ctgctcgagc
     6601 aggcgttgct catctgacaa gtcgaagttg agcatcaggc tggtcgcgtg atcgccgtag
     6661 gcgggctgga cgtctcggac gtttttcgaa agaggacctc gcggagattg gtctggagca
     6721 ggaacgactc gacgacgcgc aacggttcga agagtcgttg gatgtcgcga ttggggagcg
     6781 gccgctgcct ggagcgggcg gccgcatagg gtcgatgttg caggtgcgtc cggttgatca
     6841 ccacgtgttt cgtgtactcc gggcgcgtgc ctggccgcgg ttccgccgtg ctgaaaaaga
     6901 ccagcaacac accctccggc cgcaggatcc tggtgagctg ctccgccagc ggcttggccg
     6961 atttcttgtc gagatagtcg aacaggtccc agcagaggat gccgtcgatg ctgctggagt
     7021 cctggggaaa ccgcttcgcg agaaacgacg gcaactgatc gagcgcgccc tcgcgcacgt
     7081 gccggtcgat gtccttcgac agctccgcga aaatgatctt gcaaccgagt tcttcgccga
     7141 agaaattgac gttggcgccg acggctggcc cgagatcgag caggacgggc tgcggcttcg
     7201 tgctgaggcc cgcgatgaag cgggccagca ccttggtcgg gtacacgggc gagtcggacg
     7261 gaccggtgtc cgaatccgcg tgttccctgc ggcgtcgaga gaacagatct gagagcgcca
     7321 ccgagttcgg tgagccgtgg tccgcgatcg gccttcaccg atcggcggcc acgtctccgc
     7381 aaccgcgtca gtgcgcggcc gcttggggct gagcctgtgc cttctcagga ggcgtcgatg
     7441 ccttctcagg cggcgtcgac acggccttct gcggctgcgc tgcgggctgc gcggcgccct
     7501 tgcgctgcat gtcgacgctg ccacggaagt gggcgccctc ggcgatggcc acgcgcggcg
     7561 cgatgatgtc gccgtcgacc gacccgccgt cccgtatgtc aaccttttcg gacgccgtca
     7621 cgttcccgtt gacttcgccg agcacgatga cagccttcgc gaacacctgc gccttgatct
     7681 tgccgttggg accgatggtg agcacgtgat ccttcaactc gatccgcccc tcgacgtgcc
     7741 cctcgaccgt caggtcctcg ctcccgttca gctcgccctt gatgacaacc gatttcccga
     7801 tgttcaccat atccctctcg atccgccgga cgtccggctg cgcggccacc ggcgcgctgg
     7861 ccggctgagt ggaggcgatc ggcgccggcg ctgccggctg tccgctcgtc ggtttcacgg
     7921 cttcatcacg cttccacatg tcgtgtcctc cgcctcgatg ggcaaattcc gagcctggcc
     7981 gccttatttg gcgctcggca ccagcgctgg acgcagcggt gtcgtcgcaa cgcgggcttc
     8041 cttggggcag gagcctagtg agtatatcgg tcactcgaaa tttctgtcta gtcgctttac
     8101 tgccgagagt tacgccagat gctagagtgg tagccggtaa ctggcagccg gcagcctggg
     8161 atgcagcgaa tctatctcga ccataacgcc accactcccc cctcccccgc cgtcgtc
//
LOCUS       DBNGSH010000349         6399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1896530, whole genome shotgun sequence.
ACCESSION   DBNGSH010000349 DBNGSH010000000
VERSION     DBNGSH010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1896530"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /gene="nrfD"
                     /locus_tag="AC16CY_08225"
     CDS             complement(<1..1083)
                     /gene="nrfD"
                     /locus_tag="AC16CY_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3353812.1"
                     /translation="MTPETAALLARTPAVIEPGHTFESVTETIGHVVLSKRTPLGWFL
                     GFFIAFVLLMVLNMTIGNLLMTGVGIWGNNVPVGWAFDIINFVWWIGIGHAGTLISAI
                     LLLFRQQWRTSINRFAEAMTLFAVLCAAMFPLLHTGRPWLAAYWLFPYPNSMGLWPQF
                     RSPLIWDVFAVSTYFTVSLLFWYTGLVPDMATLRDRAKSRFAQVIYGMMSLGWRGSAR
                     HWQRYEMAYLLLAGISTPLVLSVHSVVSFDFAVSVIPGWHTTIFPPYFVAGAIYSGFA
                     MVMTLAIPLRKFYHLEDFITMRHIHNMTKVMLVTGMIVAYGYANEAFFAWYSASPHEA
                     AMMANRMGGPYAFFYWSLIAINAVMIQ"
     gene            complement(1107..4202)
                     /locus_tag="AC16CY_08230"
     CDS             complement(1107..4202)
                     /locus_tag="AC16CY_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HIR3353813.1"
                     /translation="MIDPKDIQALRAKLDGTRGRDYWRSLEAVAETPEFTEFLHREFP
                     QNASEWNDPVGRRGFLKLMSASLALAGVSACTRQPEEAIVPYVRQPEELVPGKPLFYA
                     TAMPFAGSGVGLLVESHEGRPTKIEGNPDHPSSLGATDLFAQASILGLYDPDRSQTIT
                     HLGEIRPFDAFAAAVRQVLEAQREQKGGGIRVLSETLVSPTLASQVDEFLGRFPQAKW
                     VQWEPFGRHNAREGSRLAFGEYVDAKYAIENADVIFSLDSDFLCVGNGALTHARAYAS
                     RRRLEGDRSRRNRLYAVESSPTNTGTKADHRLLLRASDVGLFARAVAARLGVAGVDAG
                     STPEAGQTWLDPLVKDLQSARGRSLVVAGEGQPPAVHALAHAMNDQLGNVGSTVVYTQ
                     TAEARPSDQRAELADLVAEMNAGTVSFLLVLGGNPVYTAPPDLNFAQALDKVPLRAHL
                     GLHHDETAALCHWHVPETHFLEMWSDVRGHDGTVTIVQPLIAPLYGGHSLHEVVSTLS
                     EAGPRPGYDIVRSHWNDQAGAPPVPARPAPTPALTPLPGSVAPSAFDKSWRRWLHDGV
                     VPNTAFAPKTVALRAGAAGPAPAPAPAGLEVVFRPDPAVYDGRFANNAWLQELPKSLT
                     KLTWDNAALIAPGTAARLGLLDVRSDGDAPSRSPIVEISYNDRVVRIPLWLSPGHAPD
                     TITLHAGYGRERAGHVANGIGVNVNALRVSGGQDILSGVNVSATGDQHPLVSTQDHWS
                     IEGRNIVRVATLQEFERDPNFAAKQEHQPLTGLTMFEARKYEGYAWGMAIDQNVCTGC
                     NSCVVACQAENNVAVVGKTQVANGREMHWLRIDRYYTGDLDNPETYHQPMLCQQCESA
                     PCEVVCPVAATIHSDEGLNDMVYNRCVGTRYCSNNCPYKVRRFNFMLYSDWNTASYKL
                     ARNPDVTVRSRGVMEKCTYCVQRINHARVTAKLEDRQIRDGEILTACQSACPTQAIVF
                     GNINDPASAVSKLKASPLNYGLLADLNTRPRTTYLAVVRNPNTELEPAQSGAEREGRQ
                     G"
     gene            complement(4207..4857)
                     /locus_tag="AC16CY_08235"
     CDS             complement(4207..4857)
                     /locus_tag="AC16CY_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3353814.1"
                     /translation="MPQIFRRSANTVSRLSLAGALMLGAGLILILIMVGRSSYVTRAH
                     EYVEQPVQFSHLHHVLDDGIDCRYCHTSVETSAFAGIPPTKTCMNCHSQIWSTAPILE
                     PVRASFRDDRPLRWIRVHDLPDFVYFNHSIHVKKGMGCETCHGRVDEMPLMRQVQSLQ
                     MEWCLNCHRNPEQYVRPRSEIFTMDYRPPVPQSVQGPMLVKEYGIRGNTSCSTCHR"
     gene            5077..5523
                     /locus_tag="AC16CY_08240"
     CDS             5077..5523
                     /locus_tag="AC16CY_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353815.1"
                     /translation="MSDRKYRQRGYQDEDRDRSPRPASPRPAPEPGAPAGARRISQDG
                     PRNINMPGFREVVRCAQCGHPTAGDIGLDSRCTRCATELHACAQCASFDPGSRFECTQ
                     PVTERVSPKNARNTCTFFSPRTTVERETTTPRTNDARKAFDDLFNF"
     gene            5534..6193
                     /gene="pdo"
                     /locus_tag="AC16CY_08245"
     CDS             5534..6193
                     /gene="pdo"
                     /locus_tag="AC16CY_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_394797918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5966..5968,aa:Sec)
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HIR3353816.1"
                     /translation="MALLSDRDRQIVREHLAVIQSPVKLVLFTQTIGAPETAFIARQV
                     LDEIAGLNDHVTVEEANVVLDRERAAQYGIEHIPSIVLLAGDDDARMRFMGAPAGYEF
                     MSLIEAVVLAGTTGSGLAPENQALIASHVTAPLDLQVFVTPTUPHCPRAVTLANRMAR
                     ESPFIRATSVEATEFMDLSRKFHVTGVPKTIVNGTIEILGALPEAMFVRGVLGLPEEA
                     T"
     gene            complement(6303..6392)
                     /locus_tag="AC16CY_08250"
     tRNA            complement(6303..6392)
                     /locus_tag="AC16CY_08250"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6356..6358),aa:Ser,seq:tga)
BASE COUNT         1104 a         2062 c         2174 g         1059 t
ORIGIN      
        1 ctggatcatc accgcgttga tggcgatgag cgaccagtag aagaacgcgt agggcccgcc
       61 catgcggttg gccatcatgg ccgcttcgtg cgggctcgcg ctgtaccagg cgaagaaggc
      121 ctcgttcgcg tagccgtacg ccacgatcat gccggtcacc agcatgactt tcgtcatgtt
      181 gtggatgtgg cgcatcgtga tgaagtcttc gaggtgatag aacttgcgca gcggaatggc
      241 gagcgtcatc accatcgcga agccggagta gatcgcgccc gccacgaagt agggcgggaa
      301 gatcgtcgtg tgccagcccg gaatgaccga gactgcgaag tcgaagctca cgaccgagtg
      361 gaccgacagc acgaggggcg tcgagatccc tgccagcagc agataggcca tctcgtagcg
      421 ctgccagtgt ctcgctgaac cgcgccaacc gagcgacatc atgccgtaga tgacctgagc
      481 aaagcgcgac ttggcgcgat cccggagcgt cgccatgtcg ggaacgagac ccgtgtacca
      541 gaacaggagc gagaccgtga aatacgtgga cacggcgaac acgtcccaga tcagcgggct
      601 gcggaactgc ggccacagcc ccatcgagtt cggatacggg aagagccagt aggccgccag
      661 ccagggccgg ccggtgtgga gcagcgggaa catggccgcg cagaggacgg cgaagagcgt
      721 catcgcctcg gcgaaccggt tgatcgacgt gcgccactgc tggcggaaga gcagcagaat
      781 cgcggagatc agcgtcccgg cgtggccgat gccgatccac cagacgaagt tgatgatgtc
      841 gaacgcccag ccgaccggta cgttgttgcc ccagatcccg acgccggtca tcaggaggtt
      901 gccgatggtc atgttgagga ccatcagcag cacgaaggcg atgaagaagc cgaggaacca
      961 gccgagcggc gtccgcttcg acagcacgac gtgcccgatc gtctcggtga ccgattcgaa
     1021 cgtgtgcccc ggttcgatga ccgccggcgt cctcgccagc agcgccgcgg tctcgggcgt
     1081 catggccgcg ggatggtgcg attccttcat ccctggcgtc cttccctctc tgcgcccgac
     1141 tgggcgggct cgagctcggt gttgggattc cgcacgacgg cgagataggt ggtccgcggc
     1201 cgcgtgttca gatcagccag cagcccgtag ttcagcgggc tggctttcag cttcgacacg
     1261 gcgctcgccg ggtcgttgat gttgccgaac acgatcgcct gcgtcgggca ggccgactgg
     1321 cacgccgtga ggatctcgcc gtcccgaatc tgccggtcct cgagcttggc agtgacgcgc
     1381 gcgtgattga tccgctgcac gcagtaggtg cacttctcca tcacgccgcg gctccggacc
     1441 gtgacgtcgg ggttgcgggc cagcttgtag ctggcggtgt tccagtccga gtacagcatg
     1501 aagttgaacc gccgcacttt gtacggacag ttgttcgaac agtaccgggt gccgacacag
     1561 cggttgtaaa ccatgtcgtt cagcccctcg tcgctgtgga tcgttgccgc gacggggcac
     1621 acgacctcgc agggcgcaga ctcgcactgc tgacacagca tgggctgatg gtaggtttct
     1681 gggttgtcga gatcgcccgt gtagtagcga tcgatgcgca gccagtgcat ctcgcgcccg
     1741 ttggcgacct gcgtcttgcc gacgacggcc acgttgttct cggcctggca ggccacgacg
     1801 cacgagttgc agccggtaca gacgttctgg tcgatcgcca tgccccaggc gtagccctcg
     1861 tacttcctgg cttcgaacat cgtcagcccg gtcagcggct gatgctcctg cttcgcggcg
     1921 aagttgggat cgcgctcgaa ctcctggagc gtcgccacgc ggacgatgtt gcgcccctcg
     1981 atcgaccagt gatcctgcgt ggacacgagc gggtgctgat cgcctgtcgc gctcacgttg
     2041 acgccggaca gaatgtcctg cccgccgctg acgcgcagcg cgttcacgtt gacgccgatg
     2101 ccgttggcga cgtgacccgc gcgctccctc ccgtagccgg catgaagcgt gatggtgtcg
     2161 ggcgcatggc cgggcgacag ccagagtgga atcctgacga cgcggtcgtt gtagctgatc
     2221 tcgacgatcg gggagcggct gggggcgtcg ccgtccgatc gcacgtccag cagaccgaga
     2281 cgcgccgccg tcccgggcgc gatgagcgcg gcgttgtccc acgtcagctt cgtgagcgac
     2341 ttcggcagct cctgcagcca cgcgttgttg gcgaagcggc cgtcgtagac ggcggggtct
     2401 ggcctgaaca cgacctcgag tccggccggt gcgggggccg gcgcggggcc tgctgcgccg
     2461 gcacgcagcg cgacagtctt cggagcgaac gcggtgttgg gaacgactcc gtcgtgcagc
     2521 catcgccgcc agctcttgtc gaacgcggac ggcgcaacgc ttccagggag cggtgtcagg
     2581 gccggggtcg gcgcggggcg agcgggaacg ggcggcgcgc cggcctggtc gttccagtgc
     2641 gagcgcacga tgtcgtagcc gggccgaggg cccgcctcgc tcagcgtgga aacgacctcg
     2701 tgaagggagt gcccgccgta gagcggggcg atcagaggct ggacgatcgt gaccgtaccg
     2761 tcgtgccccc gcacgtcgct ccacatctcg aggaagtgcg tttccggaac gtgccagtgg
     2821 cacagcgcgg ccgtctcgtc gtggtgcagg cccaggtgcg cgcgcagcgg caccttgtcc
     2881 agggcctggg cgaaattcag gtccggtgga gcggtataaa ccgggttgcc gccgaggacg
     2941 agcagaaagc tcaccgtgcc cgcgttcatc tcggcaacga gatcggcgag ctctgcccgc
     3001 tggtcgctgg ggcgcgcttc ggccgtctgc gtatagacga cggtgctgcc cacgttgccg
     3061 agctggtcgt tcatcgcatg agccagcgcg tggacggccg gaggctggcc ctcgccggcg
     3121 acaaccaggc tgcggccgcg ggccgactga aggtccttga cgagcgggtc cagccatgtc
     3181 tggccggctt cgggagtcga gccggcgtcc acgcccgcga caccgagccg ggcggcgacg
     3241 gcgcgcgcaa acaggccgac gtccgacgct cgcagcagca gccggtggtc ggccttcgtt
     3301 cccgtgttgg tcggcgagct ctcgactgcg tacagccgat tgcgccgcga gcgatcgcct
     3361 tcgagccgcc ggcgcgacgc gtaggcgcga gcgtgcgtca gcgctccatt gccgacgcag
     3421 aggaaatccg agtcgagcga gaagatgacg tcggcgttct cgatcgcgta cttggcgtcc
     3481 acgtactcgc cgaaggcgag tcggctgccc tcgcgcgcgt tgtgacgccc gaacggctcc
     3541 cactgaaccc acttggcctg cgggaagcgg ccgaggaact cgtccacctg cgacgcgagc
     3601 gtgggcgaga cgagcgtctc gctgagcacg cggatgccgc cgcccttctg ctcccgctgt
     3661 gcctcgagga cctgacgcac cgccgcggcg aacgcgtcga acggccgaat ctcacccagg
     3721 tgcgtgatcg tctgagagcg gtcgggatcg tagaggccga gaatggaggc ctgcgcgaac
     3781 aggtccgtgg ccccgaggct cgacgggtga tcgggattcc cctcgatctt ggtcgggcgg
     3841 ccttcgtggc tctcgacgag caggccgacg ccggaccccg caaacggcat cgccgtcgcg
     3901 tagaagagcg gcttccctgg gacgagctct tcgggctgac ggacatacgg aacgatcgcc
     3961 tcttcaggct gccgcgtgca agcgctgacg ccggcgaggg ccagcgacgc gctcatcagc
     4021 ttcaggaagc cccggcggcc gacagggtcg ttccactcgg aggcgttctg cggaaactcg
     4081 cgatggagaa actcggtgaa ctccggcgtc tcggcaacgg cttccaggct gcgccaatag
     4141 tcgcggccgc gggtgccatc gagcttcgcg cgaagagcct gaatgtcctt cgggtcgatc
     4201 atgtggtcac cggtgacagg tggagcagct ggtgttgccc ctgatcccgt actccttgac
     4261 gagcatcgga ccctgcaccg actgcggcac cgggggacgg tagtccatgg tgaagatctc
     4321 gctgcgcggc cggacgtatt gctccgggtt gcgatggcag ttgagacacc actccatctg
     4381 cagtgactgc acctgcctca tgagcggcat ctcgtcgacg cggccgtggc aggtctcgca
     4441 tcccatcccc ttcttcacat ggatgctgtg gttgaagtac acgaagtcgg gcaggtcgtg
     4501 aacgcggatc cagcgcagcg gccggtcgtc tcgaaagctg gcccggactg gctcgaggat
     4561 cggcgcggtg gaccagatct gcgagtggca gttcatgcac gtcttggtgg gaggaatccc
     4621 ggcgaaggcg gaggtttcga ccgacgtgtg gcaataccgg cagtcgattc cgtcgtccag
     4681 gacgtggtgg agatggctga actgaacggg ttgctcgacg tactcgtggg cgcgggtgac
     4741 gtaggacgag cgcccgacca tgatcaagat caggatcagg ccagccccga gcatcaaagc
     4801 gccggctaga ctcagcctcg agacagtgtt cgcgcttcga cgaaagattt gcggcatcgg
     4861 ttattgatgg ggtgacctgg cgggcggcgt ccctccgtgg cccggtccga tttttaccac
     4921 attgtgaaaa aaggatcaaa cggcgccggg gacctgtcag gcccgaacgc gacggcgcga
     4981 ttctagcgga aacggtcggc gacttcacac ctcttcacag aatctatcgg gcctgcaaac
     5041 cagcccgacg gtatacaatc cgcccaagcc gacgccatga gcgacagaaa gtaccgacag
     5101 cggggctacc aggacgagga ccgggaccgg agtccacggc ctgcctctcc ccgccccgcc
     5161 ccggagcccg gggccccggc tggcgcccgc cgcatctccc aggacggacc ccggaacatc
     5221 aacatgcctg ggtttcgtga agtcgtccgc tgcgcgcagt gcggccaccc gactgccggc
     5281 gacatcgggc tcgactcgcg atgcacccgg tgcgccaccg agctgcacgc gtgcgcgcag
     5341 tgcgcgtcct tcgacccggg aagccgattc gagtgcacgc agccggtgac cgaacgggtc
     5401 agcccgaaga acgcccggaa cacgtgcacg ttcttttctc cgaggacgac agtcgaacgc
     5461 gagacgacga cgccgaggac caacgacgcc aggaaggcgt tcgacgatct cttcaacttc
     5521 taaggagccc cgcctggctc tcctctcaga tcgggatcgg cagattgtcc gcgagcatct
     5581 cgcggtgatc cagtcgccag tcaagctggt gttgttcacg cagacaatcg gggcgccgga
     5641 gacggcgttc atcgcccggc aggtgctcga cgagatcgcc ggtctcaacg atcacgtcac
     5701 cgtcgaagag gcgaacgtcg tcctcgatcg agagcgcgcg gcacagtacg gaatcgagca
     5761 catcccgtcg atcgttctgc tcgccggcga cgacgacgcc cggatgcgct tcatgggtgc
     5821 tccggcaggc tacgagttca tgtcgctcat cgaagccgtc gtgctcgccg gcaccaccgg
     5881 ttcgggcctg gcgcccgaga atcaggcgct catcgcctcg cacgtcaccg caccgctcga
     5941 tctgcaggtc ttcgtcacac cgacgtgacc gcactgcccg cgagccgtga cgctcgcgaa
     6001 caggatggcg cgggaaagcc cgttcattcg tgccacctcc gtggaagcca ccgagttcat
     6061 ggacctttcg cgcaagttcc acgtgacggg cgtacccaag acgatcgtca acgggacgat
     6121 cgaaattctc ggtgcgctgc cggaagcgat gttcgtgcgc ggcgtgctcg gactgccgga
     6181 agaggcgacg tgagcgtgcg tccgccttcg cgcttcgcgc tacggtggac aacattcgat
     6241 aacgctcgcg tgacgcggct tgccaaccga agccgcgagc gaagcgagcg tttggcgaag
     6301 gttggcggag ggagagggat tcgaaccccc gggaccgttt ccgatccaac ggttttcaag
     6361 accgccgcct tcaaccactc ggccatccct ccgtgcgcg
//
LOCUS       DBNGSH010000350         2778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4613963, whole genome shotgun sequence.
ACCESSION   DBNGSH010000350 DBNGSH010000000
VERSION     DBNGSH010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4613963"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="AC16CY_08255"
     CDS             <1..309
                     /locus_tag="AC16CY_08255"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3353817.1"
                     /translation="GTVTKFQWTNPHGFIELDVAQPDGTIKHWSIETTSINMMQRLGW
                     RSNMIKAGDKLKVVAAPLLNGDPAALLLEVTFPDGKKMEPGVPGANSFIRTPDPVEYK
                     "
     gene            306..1082
                     /locus_tag="AC16CY_08260"
     CDS             306..1082
                     /locus_tag="AC16CY_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353818.1"
                     /translation="MTASSRLATAIVTGFLVLGGSAGAQGVPSQARPVPDITGSWQRL
                     GATVGVRPTAKPDPKLLPPPAAQPVLKPQYQQERQQRLQAIREADAKGQPLANHNVYC
                     IPDGMPAMMNAVFPMEILQSRGQVTIIAEAYNQVRRILMDRPQKTLDEVELTFYGHAV
                     GRWEADTLVVDTIGIKENVRFQNTPHSAQMRIRERMRLVAPDILWNEITIEDPVTLEQ
                     PWTFTYAYQRMPDYTLLEYICEDNREYSDEKGLQQIRIAK"
     gene            1097..1648
                     /locus_tag="AC16CY_08265"
     CDS             1097..1648
                     /locus_tag="AC16CY_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR3353819.1"
                     /translation="MSGTAPILLLSMPQMADPNFAKTVVLLCDYTAEDGAFGLVVNRQ
                     MEEPACEVVRTEPPVRVDPDLRLWIGGPVDPQSTWVLTTDSLGPDDEQRQIAPGVLLS
                     VSRSLALRLLQEPPSSRTKVVVGYAGWRPGQLENEIAASSWLTTDVDPGLIFGVPPDQ
                     MWEKAIRRLGADPANLQTSSGVH"
     gene            1757..>2778
                     /locus_tag="AC16CY_08270"
     CDS             1757..>2778
                     /locus_tag="AC16CY_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl-peptidase 3 family protein"
                     /protein_id="HIR3353820.1"
                     /translation="MKLTTLLTLVLVAAAPACSRQQTTQPTVQTNAPPSDPETQKLQT
                     MAARFAPVDLVVDLTPMPENERQALARLVQAAKVFDALFLRQVWEGNESMLLDLVRDD
                     SPLGRARLHYFRINKGPWSRLDGNEPFIAGVPEKPAQANFYPAGASKADVEAWIKTLG
                     PAERARATGFFTTIRRGPDGRFMAVPYSLEYQGEIARVAALLREAAALTKQPTLKTYL
                     EKRAAALLGNDYYESDVAWMELDASIEPTIGPYEVYEDEWFNYKAAFEAFITLRDDAE
                     TKKLARFGAELQEIENNLPIDPKYRNPKLGALAPIRVVNTVFSSGDGNRGVQTAAFNL
                     PNDERVV"
BASE COUNT          524 a          921 c          891 g          442 t
ORIGIN      
        1 ggcacggtga cgaagttcca gtggaccaac cctcacggat tcatcgagct ggacgtcgcc
       61 cagcccgatg gcaccatcaa gcactggagc atcgagacga cgagcatcaa catgatgcag
      121 cggctcgggt ggcgttcgaa catgatcaag gcgggggaca agctgaaggt cgtcgcggcg
      181 ccgcttctca acggcgatcc ggccgcgttg ctgctcgaag tcacgttccc cgacggcaag
      241 aagatggagc ccggggtccc gggggcaaac tcgttcatcc gtacgcctga cccggtggag
      301 tacaagtgac ggcctcttcg cgtctcgcga cggcgatcgt caccgggttc ctcgtcctcg
      361 gcgggagcgc gggtgcgcag ggcgtgcctt cgcaggctcg accggttccc gacatcaccg
      421 gctcgtggca gcgccttggg gcgacggttg gcgttcgccc caccgcgaag cccgatccaa
      481 agctgcttcc tcctccggcg gcacagccgg tcttgaaacc gcaataccag caggagcgtc
      541 agcagcggct gcaggcgatc cgcgaggccg acgcgaaagg gcagccgctt gccaaccaca
      601 acgtctactg cattccagac ggcatgccgg cgatgatgaa cgcggtgttc ccgatggaga
      661 tcctgcagag ccgcgggcag gtcaccatca tcgcggaagc gtacaaccag gtgcgccgca
      721 tcctgatgga ccggcctcag aagactctgg acgaggtgga gctgaccttc tacggccacg
      781 ccgtggggcg gtgggaagcc gacacgctcg tcgtggacac gatcggcatc aaggagaacg
      841 tgcgattcca gaacaccccg cactccgccc agatgcgtat acgcgagcgc atgcgcctgg
      901 tggcgccaga cattctgtgg aacgagatca cgatcgagga tccggtgacg ctcgagcagc
      961 cgtggacgtt cacctacgca tatcagcgca tgcccgacta cacgctgctc gagtacatct
     1021 gcgaggacaa ccgcgagtac tccgacgaga agggccttca gcagatcagg attgcgaagt
     1081 aacctatcaa cagcccatgt caggcaccgc ccccatcctc ctcctgtcga tgccccagat
     1141 ggcggatccg aacttcgcca agaccgtcgt cctcctgtgc gattacacgg ccgaagacgg
     1201 cgccttcggg ctcgtcgtca accgccagat ggaggagccg gcgtgcgagg tggtccggac
     1261 ggagccgccc gtgcgcgtcg atcccgacct ccggttgtgg atcggcggac ctgtcgatcc
     1321 gcagagcacg tgggtgctga cgaccgactc gctgggacct gacgacgagc agcggcagat
     1381 cgctcccggc gtgctgctgt cggtatcccg ctcgcttgcc cttcggctcc tgcaggagcc
     1441 accgtcgagc cggacgaagg tcgtcgtggg gtacgctgga tggagacccg gacagctcga
     1501 gaacgagatt gccgcgtctt cgtggttgac gaccgacgtc gatcccggac tcatcttcgg
     1561 cgtgcccccg gaccagatgt gggaaaaggc gattcgccgt ctcggagcgg atccggcgaa
     1621 tctgcagacc agctccgggg tgcattgatc cggatcacga accacggaga cacagaggca
     1681 cggagccagg gactcttgga ttcattttct ccgtgtctcc gcgcctccgt ggtttcgtgc
     1741 gtcttcgaat gacgtgatga agctcacaac tcttctgacg ctcgtgctcg ttgccgctgc
     1801 gcccgcgtgc agtcgtcaac agaccacaca accaaccgtg cagacaaacg cccctccgtc
     1861 agatcctgaa acgcagaagc tccagaccat ggccgcgcga ttcgcgcccg tggacctcgt
     1921 ggtcgacctg accccgatgc ccgagaacga gcgccaggcg cttgccaggc tcgtgcaggc
     1981 cgccaaggtg ttcgacgccc tgttcctgag gcaggtctgg gagggcaacg agtcgatgct
     2041 gctcgacctc gtgcgcgacg attccccgct cggccgcgcg cggctgcact acttcaggat
     2101 caacaagggg ccctggtcgc ggctcgacgg caacgagccg ttcatcgccg gcgtgcccga
     2161 gaagccggcg caggccaact tctatcccgc cggcgcctcg aaggccgacg tcgaggcgtg
     2221 gatcaagacg ctcgggccgg ccgagcgggc ccgcgcgacc ggcttcttca ccacgatccg
     2281 ccggggcccg gacgggcgct tcatggccgt gccgtacagc ctcgagtacc agggagagat
     2341 cgcgcgcgtg gccgcgctgc tgcgcgaagc ggcggcgctg acaaagcagc ccacgctcaa
     2401 gacctatctc gagaagcggg cggcggcgct cctcggcaac gactactacg agagcgacgt
     2461 cgcctggatg gagctcgacg ccagcatcga gccgaccatc gggccctacg aggtgtacga
     2521 ggacgagtgg ttcaactaca aggccgcctt cgaagcgttc atcacgctgc gcgacgatgc
     2581 cgagacgaag aagctggcga gattcggcgc cgagctgcag gagatcgaga acaacctgcc
     2641 cattgatccc aagtaccgca atcccaaact cggtgccctg gcgccgattc gcgtcgtcaa
     2701 tacggtcttc tcatcgggtg acggcaatcg gggcgtgcag acggccgcct tcaacctgcc
     2761 gaacgacgag cgcgtggt
//
LOCUS       DBNGSH010000351         4990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_29443, whole genome shotgun sequence.
ACCESSION   DBNGSH010000351 DBNGSH010000000
VERSION     DBNGSH010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_29443"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="AC16CY_08275"
     CDS             complement(<1..630)
                     /locus_tag="AC16CY_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HIR3353821.1"
                     /translation="MSNESKCPFSGKAHSGTSNREWWPKQLNLKALHQNPPAGDPMGK
                     DFNYAEEFKTVDLDALKKDIEKVMTTSQDWWPADYGHYGPLFIRMAWHSAGTYRIHDG
                     RGGAGSGTLRFAPLGSWPDNANLDKARRLLWPIKQKYGRKISWADLMVFTGNCALESM
                     GFKTFGFAGGREDVWEPEQDVYWGSERTWLGDERYSGDRQLENPLAAVQM"
     gene            complement(667..1188)
                     /locus_tag="AC16CY_08280"
     CDS             complement(667..1188)
                     /locus_tag="AC16CY_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019931753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3353822.1"
                     /translation="MATDPADLLRQRGIQVTAQRLAVLRAVAGQPHAAADAVAESVRA
                     QIGAISLQSVYDCLGVLVAEGLVRRIQPSGSPARFETRVGDNHHHLICRVCGRMVDVD
                     CAVGSAPCLTAADGMGYEIDEAEVVYWGRCPDCQVKSRKPSRARQRLRADTASPAHSS
                     RRPRQRATRRRPD"
     gene            1312..1815
                     /locus_tag="AC16CY_08285"
     CDS             1312..1815
                     /locus_tag="AC16CY_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3353823.1"
                     /translation="MMRRFVLAVVLVSVASLVHAQEPVVGVKDPESLFKDADPVLNRN
                     KQAALHIMRELLQCNQWDRAGEWLTQRYIQHNPNAASGLDGVVAFFTKVMKRQRVDKC
                     DRLTTEVVAVMADDDYVTVMMPRRYPDPRKPGAEYFTTWFDTWRFVNGKADEHWDPAT
                     IAAPAAK"
     gene            complement(1819..2499)
                     /locus_tag="AC16CY_08290"
     CDS             complement(1819..2499)
                     /locus_tag="AC16CY_08290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3353824.1"
                     /translation="MNVHCGTALVLTVLVVVPAGQTAPPMRPTDEQITLADVEVRRLV
                     PLLRLEPGMRIADVGAGLGAWTLRFAQWTGPSGHVYSTDISEEQLTALRALAARERLS
                     NVTVIRGAAASTNLPAGCCDAILMRNVFHYVTEPAAMIRSLAESLKPGGRLAVVDFPP
                     RPNTAPPAGVPTNRGGQGVPPEVVESEVGALLRHVTTVPNWAPEGVPAWVPKEIAPPF
                     AAIFEKAR"
     gene            2540..3469
                     /locus_tag="AC16CY_08295"
     CDS             2540..3469
                     /locus_tag="AC16CY_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353825.1"
                     /translation="MKSLVLLVAVLAFARGAAGQDADDYRGGWRTDGGEAHTYQFSIR
                     GVAVRGIYCTYCADATTLAFVDGTLGPGGIRFEVTHVNADGSTRYKDQAVATVSQGRL
                     IVTGTSGAPGRRRFERVLIKDPRGPDPIPVKVSVLPKDRPPVPANPVRRNPDGADGLR
                     YIQPGPWKSTLTEKDVVGVWLGFGVGAPKQFFIIKKVGSRLRGMVCGTCDNPYTMAAL
                     DDFRIEGDTLTFNILHEDWGDGEIPTFDKHVTAHVGWNEMRCTTTADHQPPPKPPAPG
                     IVPGFSLQGPVPIEATSGNVWPEWPPKNTSSPR"
     gene            3543..3779
                     /locus_tag="AC16CY_08300"
     CDS             3543..3779
                     /locus_tag="AC16CY_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR3353826.1"
                     /translation="MAALNWSQCPAVERIPGKVSGAWVLKGTRMPVATIFENLEAGAS
                     IDDVLTWYEGLDRKQVQAVIEFAARSLDLAATER"
     gene            3935..4111
                     /locus_tag="AC16CY_08305"
     CDS             3935..4111
                     /locus_tag="AC16CY_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353827.1"
                     /translation="MTTDKNLKHQQDLSKRSIAIIVIGHAQWRGLEAHVHLVVAAIDR
                     AAKGSYDEVEIPVG"
     gene            complement(4127..>4990)
                     /locus_tag="AC16CY_08310"
     CDS             complement(4127..>4990)
                     /locus_tag="AC16CY_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353828.1"
                     /translation="GCALMPVEAGLQTRLPITTIDLGGATLLQGAGGNVIALPGPDGA
                     LMIDGGLTANADALVAAVLKATGSSRVHTLINTHWHPDVTGANDAVGRAGGVIFAHEK
                     TKVTLSSTVYSAIGFTSRLSPLPEAARPTRTTRGDGSMEFAGQQVDYGYMPAAHTDGD
                     LFVYFPKLNLLATGGVVSAERWPLIDHRNGAWLGGRVRALERLASLVKPDTRVVAADG
                     RMMTGKEIARHRDMYQTLFATMIGYLNMGLGPEDAVTRNPLREYEKEFGDASDFEYAA
                     LRSMMIAYVPD"
BASE COUNT          823 a         1622 c         1695 g          850 t
ORIGIN      
        1 catctgtacg gccgccaggg gattctcgag ttgacggtcg ccgctgtagc gctcgtctcc
       61 gagccacgtg cgctcagagc cccagtagac gtcctgctcg ggctcccaga cgtcctcgcg
      121 ccctcccgcg aagccgaacg tcttgaagcc catcgactcc agggcgcagt tgcccgtgaa
      181 gaccatcagg tcggcccacg agatcttccg gccgtacttc tgcttgatcg gccagagcag
      241 ccggcgcgcc ttgtcgaggt tcgcgttgtc cggccagctg ccgagcggcg cgaagcgcag
      301 cgtgcccgat ccggccccgc cgcggccgtc gtggatgcgg tacgtgccgg cgctgtgcca
      361 cgccatccgg atgaagagcg gcccgtagtg gccgtagtcc gccggccacc agtcctgcga
      421 cgtcgtcatc accttctcga tgtccttctt gagggcgtcg agatcgaccg tcttgaactc
      481 ttcggcgtag ttgaagtcct tgcccatcgg atcgcccgcg ggcgggttct ggtggagggc
      541 cttcaggttc agctgctttg gccaccactc tcgattcgac gtgccgctgt gtgctttgcc
      601 agagaaggga cacttgcttt cgttactcat ggacgttgac ctcttccagg aatgggagtg
      661 cccggctcag tccgggcgtc ttcttgtggc ccgctgacgg ggccgtcggg atgaatgtgc
      721 gggggacgcc gtgtccgccc tcaggcgttg ccgcgcgcgg gatggcttcc gcgacttcac
      781 ctggcaatcc gggcaccgac cccagtacac gacttcggct tcgtcgatct cgtagcccat
      841 gccgtcggcg gcggtgagac agggggccga gccgaccgcg cagtcgacgt ccaccatgcg
      901 gccgcagacg cggcagatca gatggtggtg gttgtcgccc acccgggtct cgaatcgcgc
      961 cggcgacccg gagggctgaa tgcgccggac gaggccttcg gcgaccagga cgccgaggca
     1021 gtcgtacacc gactggagcg agatggcccc gatctgggcc ctgacggatt cggccacggc
     1081 gtcggccgcg gcatggggct gacccgcgac agcccgcaag acagccaatc gctgggccgt
     1141 cacctgaatg ccgcgctggc gaaggaggtc cgccgggtcg gtggccacgg agccatatta
     1201 gatcaaataa ttgattcgtt caaatattgt gagcactcgt ggcgaagcgg cggcattcct
     1261 gcgctagaat cgccagcgct tcccctccct actctttgtg aggactcgac gatgatgcga
     1321 cggtttgtgt tggcagtagt gctcgtgtca gtggcctccc tggttcatgc ccaggagccc
     1381 gtggttggcg tgaaggatcc cgagtcgttg ttcaaggacg cggatccggt gctcaaccgc
     1441 aacaagcagg cggcgctgca catcatgcgc gagctgctgc agtgcaacca gtgggatcgc
     1501 gccggcgaat ggctgacgca gcgctacatc cagcacaacc cgaacgccgc gtcggggctc
     1561 gacggcgtcg tcgccttctt caccaaggtg atgaagcgcc agcgcgtgga caagtgcgat
     1621 cggctgacca cggaagtcgt cgccgtgatg gccgatgacg actacgtcac ggtgatgatg
     1681 ccgcgccggt atcccgatcc gcggaagcca ggcgccgagt acttcacgac gtggttcgac
     1741 acctggcggt tcgtcaacgg caaggccgac gagcactggg atccggcgac gattgccgcg
     1801 ccggcggcaa agtagcagct acctggcctt ctcgaagatc gcggcaaacg gcggggcgat
     1861 ttccttcggc acccacgccg gtacgccctc gggtgcccag ttgggcacgg tggtgacgtg
     1921 cctgagaagg gcgccgacct cgctctcgac gacttcgggg ggcacgccct ggccgccgcg
     1981 gttggttggc acgccggcag gcggcgccgt gttgggtctg ggtgggaaat ccacgaccgc
     2041 gagccgcccg ccgggcttca acgactcggc gaggctgcga atcatcgcgg caggttcggt
     2101 cacgtagtga aagacgttgc gcatcaggat ggcgtcgcag cacccggccg gcaggttggt
     2161 cgaggcggcg gcgcccctga tgaccgtcac gttcgacagc cgttcccgcg ccgcgagagc
     2221 ccgcagcgcc gtcagctgct cctcactgat gtcggtcgag tagacgtgcc cggatggccc
     2281 ggtccactgg gcgaagcgca gcgtccacgc gccgagcccc gcgccaacgt ccgcgatcct
     2341 catgcccggc tcgagccgga ggagcggcac gagccgccgg acttcgacgt cggcaagcgt
     2401 gatctgctcg tccgtgggac gcatcggcgg cgccgtctgg cctgccggaa cgaccacgag
     2461 gacggtgaga acgagcgcgg tgccacaatg cacgttcatc gtcgtacatg ctagcgtgtt
     2521 caggtctcag ggggcgttca tgaaatcgct ggtattgctg gtcgccgtac tggccttcgc
     2581 acgcggggcc gcgggtcagg acgcggacga ttatcgcggc ggctggagaa ccgacggcgg
     2641 cgaggcgcac acctaccagt tctcgattcg gggcgtggcg gtcagaggca tctattgcac
     2701 ctattgcgcc gatgccacaa cgctcgcgtt cgtcgatggg acgctgggcc ccggcggaat
     2761 caggttcgag gtcacgcatg tgaacgcgga cggcagcacg aggtacaagg atcaggccgt
     2821 cgccaccgtc agccagggac gattgatcgt gacgggcacg agcggcgcgc cgggccgccg
     2881 gcggttcgag cgcgtgctca tcaaggatcc gcgcggaccg gatccgatcc ctgtgaaggt
     2941 gtccgttctc ccgaaggatc gcccgccggt gccggcgaac cctgttcgtc gaaacccgga
     3001 cggcgcggac ggcctgcgct acatccaacc gggtccgtgg aagtcgacgc tgacggagaa
     3061 agacgtggtc ggcgtgtggc tgggattcgg cgtgggggcg ccgaagcagt tcttcatcat
     3121 caagaaggtc ggcagccggc ttcgcggcat ggtgtgcggg acgtgcgaca acccgtacac
     3181 gatggcggcg ctcgacgatt tccggatcga aggagacacg ctgacgttca acatccttca
     3241 cgaggactgg ggcgacgggg agatccccac cttcgacaag cacgtgaccg cgcacgtggg
     3301 ctggaacgag atgcggtgca cgacgaccgc ggaccaccag ccgcccccaa agccgccggc
     3361 cccgggcatc gtcccggggt tctccttgca gggcccggtt ccgatcgagg cgacgtcggg
     3421 aaacgtctgg cccgagtggc cgccgaagaa cacctcgtcg ccgcgctagg cggcttcaat
     3481 ccggcacgtc ccatgttcag ggagtcagcc acatccttgg cttcggtgta atctgtgacg
     3541 gtatggcagc gttgaattgg tcgcaatgtc ccgcggtgga acgtattcct ggcaaggtca
     3601 gcggtgcctg ggtgttgaag ggtacccgca tgccggtcgc gacaatcttc gagaaccttg
     3661 aggcgggagc cagcatcgac gacgtgctca cttggtacga gggcctcgac cgcaagcagg
     3721 tgcaggcggt tatcgagttc gcggcgcgga gccttgacct ggctgcgaca gagcgttagg
     3781 ccagatgcgg gtcttgctgg accaagggac acccctacca ctcaggcagc acctccttca
     3841 tcacgcggtc cggacagcct tccaagaagg ctgggcgacg ctcgccaacg gtgacctgct
     3901 aacggccgcg gagtccgctg ggttcgaggt gttcgtgacg acggacaaga acctgaagca
     3961 ccagcaggat ctctccaagc gcagcatagc aatcattgtc atcgggcacg ctcagtggcg
     4021 tgggctcgag gcccacgtcc acctcgtcgt ggcggccatc gatcgagctg ccaagggcag
     4081 ctacgacgaa gtcgagatcc ccgtcggctg aattacgcta cgcacctcaa tcgggcacgt
     4141 aggcgatcat catgctccgc agggccgcgt actcgaagtc cgacgcgtcg ccgaactcct
     4201 tctcgtattc cctgagcggg ttccgcgtga cggcgtcttc aggaccgagc cccatgttca
     4261 ggtagccaat catcgtcgcg aacagcgtct gatacatgtc gcggtgccgc gcgatctcct
     4321 tgccggtcat catgcggccg tcggcagcca ccacgcgcgt atctggcttg acgagactgg
     4381 cgagccgctc cagcgcgcga acgcggccgc ccagccacgc gccgttccgg tggtcgatga
     4441 gcggccatcg ctccgcggac acgactcccc cggtggccag caggttcagc ttgggaaagt
     4501 agacgaagag atccccgtcg gtgtgcgctg cgggcatgta gccgtagtcc acctgctgcc
     4561 ccgcgaactc catggagccg tcgccgcgcg tggtccgggt cggccgggcg gcttcaggca
     4621 gaggggacag gcggctggtg aagccgatag cggagtacac cgtgctgctc agcgtcacct
     4681 tcgtcttctc gtgggcgaag atgacgccgc ccgcccggcc cacggcgtcg ttggcgccgg
     4741 tcacgtccgg atgccagtgc gtgttgatca gcgtgtggac gcggctgctg ccggttgcct
     4801 tcaacacggc ggccacgagg gcatcggcgt tcgccgtcag gccgccgtcg atcatgagcg
     4861 cgccgtccgg accaggcagc gcgatcacgt tgccgcccgc gccctggagc agcgtcgcgc
     4921 caccgaggtc gatcgtcgta attggtaggc gggtctgaag acccgcctct acgggcatca
     4981 gcgcgcagcc
//
LOCUS       DBNGSH010000352         1517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2067015, whole genome shotgun sequence.
ACCESSION   DBNGSH010000352 DBNGSH010000000
VERSION     DBNGSH010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2067015"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="AC16CY_08315"
     CDS             complement(<1..152)
                     /locus_tag="AC16CY_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017992313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR3353829.1"
                     /translation="MSHKSLEDLLQAVGSPVKLLRNSQTGPYIYPVVPSEFSNWRDEQ
                     RAWRET"
     gene            308..733
                     /locus_tag="AC16CY_08320"
     CDS             308..733
                     /locus_tag="AC16CY_08320"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3353830.1"
                     /translation="MTSRMTSASLMIVAVAVGLLAASATARAHHGTANVYDSKAIVLK
                     GTITKFEWTNPHNQIYFDVKDEKGNVVHWIAATEPPQVMLERGWTRRSLKEGDQVTMY
                     VFAAKNGAPVGNVQRIVLADGKELTTFGPRPAEAAPPAK"
     gene            756..>1517
                     /locus_tag="AC16CY_08325"
     CDS             756..>1517
                     /locus_tag="AC16CY_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353831.1"
                     /translation="MRNTFTVLAALGVGIVTFSLVLRTPAAAAAPAQGQGASAKVPDL
                     SGDWAPDGRRGGIGQSMSIADNGGKQRGKEPDIPYQPWAREKTMSERPSTGPEPFFGS
                     TTDPQVLYCEPPGVPHIYLWPIKTKFIQTPEAVYILHEYGPFYRIVWLNSKHPEDPDP
                     QWWGHSIGWYENGDTLVVDTVGFNGKTWLDQMGHPTTEQLHLTERYKRLDANSLELKM
                     VIDDPGAYTQPWNAQRYFTLSKTGFLRYQQICSVRE"
BASE COUNT          295 a          502 c          468 g          252 t
ORIGIN      
        1 cacgtctccc gccaggcgcg ctgctcgtct ctccaattcg agaactcgct cgggaccacg
       61 gggtagatgt acggcccggt ctgcgagttg cgcagcagct tcaccgggct cccgacggcc
      121 tgcagcaagt cctccaagct cttgtggctc atttccctgt ctccctccca tctagtggaa
      181 accatgatga tctcccacag cgggacgatc gcgcaaatac gagaaggggt cagacccctt
      241 cacatggccg cgcacgcggt ataatccgcc cgatccgccg tcgccagttc cagtctggag
      301 gcaacccgtg acgagcagaa tgaccagtgc atcactcatg atcgtggccg tggccgtcgg
      361 ccttctcgcg gcgtccgcca ctgcgcgcgc gcaccatggg accgccaacg tctacgactc
      421 caaggccatc gtgctgaagg ggaccatcac gaaattcgag tggaccaacc cccacaatca
      481 gatctacttc gacgtcaagg acgagaaggg caacgtggtc cattggattg ccgccaccga
      541 gccgccccag gtgatgctcg agcgcggctg gacgaggcgc tcgctgaaag agggcgatca
      601 ggtcacgatg tacgtgttcg ctgccaagaa cggcgcgccg gtcggcaacg tgcagcgcat
      661 cgtcctcgcg gacggcaagg agctgaccac gttcgggcct cggcctgccg aagccgcgcc
      721 gcccgcgaag taactctggc gtcaggagtc attccatgcg gaacacgttc acggttctcg
      781 cggcgctcgg tgtcggcatc gtcaccttct cgctcgtgct gcggacgccg gcggctgctg
      841 cggcgccggc tcaggggcag ggtgcatccg cgaaggtgcc ggacctctcc ggcgattggg
      901 cgcccgatgg caggcgagga ggcatcggcc agagcatgag catcgccgac aacggcggaa
      961 agcagagggg caaggaaccc gacatcccgt accagccgtg ggcacgcgaa aagacgatgt
     1021 ccgagcggcc gtcaaccggt cccgagccgt ttttcggcag caccaccgat ccgcaggtgc
     1081 tgtactgcga gccgcccggc gttccccaca tctacttgtg gccgatcaag accaagttca
     1141 tccagacgcc cgaggcggtg tacatccttc acgagtacgg tccgttctac cggatcgtct
     1201 ggctgaacag caaacatccc gaagacccgg atcctcagtg gtgggggcac tcgatcggct
     1261 ggtacgagaa cggcgacacg ctcgtcgtgg acaccgtcgg tttcaacggc aagacgtggc
     1321 tggatcagat gggccatccg accaccgagc agctccatct gaccgaacgg tacaagcgcc
     1381 tggacgcgaa ctcactcgag ctgaagatgg tgatcgacga ccccggggcg tatacgcagc
     1441 cctggaacgc acagcggtat ttcacgctgt cgaagaccgg ctttctgcgc taccagcaga
     1501 tctgctcggt tcgtgag
//
LOCUS       DBNGSH010000353         2730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2916084, whole genome shotgun sequence.
ACCESSION   DBNGSH010000353 DBNGSH010000000
VERSION     DBNGSH010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2916084"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2730
                     /locus_tag="AC16CY_08330"
     CDS             <1..>2730
                     /locus_tag="AC16CY_08330"
                     /EC_number="1.14.-.-"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0019184 - nonribosomal peptide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0044550 - secondary metabolite biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HIR3353832.1"
                     /translation="QRPLGSLGLDSLKAVELQHGLERELSITLPMADVLSARHVADLV
                     SGVLEKMIAGAPSSSKTQPGAPPAPAADTSRLSRGQEALWFLQQLSPDSAAYNIAYAM
                     RVRSAVDSSKLRRTLQVLVDRHACLRTRFENRNGRPWADVRERDEVSFTEVDAAGWTD
                     ARLQQYLTEQSHRPFDLARGPLFTVHLLTRSARDHVLLICVHHISVDFWSLVVLTDEM
                     GRLYGAEDPQAEAKQLPLPAPYAEFVRWQDGMLGSDPGREHETYWLKQLSGTLPVLNL
                     PTDRPRTPVQTYRGASHTFQLGPELTRALHDLARQQQVTLYSLLLATFQSLLHRYTGD
                     ADVLVGSPVAGRSREGFGGVVGYLVNMVALRGDLSDDPTFTELLSRTRAIVLGALAHQ
                     DFPFALLVDRLEQNRDPSRSPVFQAAFVLEKPHIREELAEFVLGESGATMSLGGLQLE
                     SLALEQRVAQFDLTLMMVEAGGGLAASIQYNADLFDPSTISRLARHYQTLVEGVSADP
                     QRRLSELPLLSVEERRQLLEWKGEVADVSPAVTLQGLFEEQVARTPDAVSVAWDGGQL
                     SYRELNRRANQLAHHLRRRGVGPETLVGVCMDRSPEMIVAILGILKAGGGYFPLDPGY
                     PRDRLSFMLRDGDVRLVLGKDQLAEASGESTENPVPLSTGQNLAYVMYTSGSTGTPNG
                     VAVSQQSVIRLVADPNYVTLTPNEVVLQLAPNTFDAATFEVWASLLHGGRLVLATDDK
                     PSLEQVSQMLAKHRVSTLWLTSGLFHQLVDHDIDAFGTVRQLLAGGDVLSVSHVRNVL
                     TRFPSCRVINGYGPTENTTFTCCYPMQGDKAAPAVSVPIGRPITGTEVFVVDRHQHAV
                     PVGVSGELLTGGTGLARGYLGRPSLTAERFVPSTLSEHGGARLYKTGDLVRYL"
BASE COUNT          443 a          899 c          892 g          496 t
ORIGIN      
        1 cccagcggcc gctcggctcg ctggggctgg actcgctcaa ggccgtcgag ctgcagcacg
       61 gtctcgagcg ggagctgagt attacgctgc cgatggccga cgtgttgtcg gcccgacacg
      121 tcgccgatct ggtatccggc gtgctcgaga agatgatcgc cggggcgccg tcatccagca
      181 agacgcagcc cggcgcgccg ccggcgccgg cagcagatac gagtcgcctg tctcgaggac
      241 aggaggcatt gtggtttctc cagcagctct cgcccgacag cgcggcctac aacatcgcgt
      301 acgccatgcg cgtacgctcg gcggtcgatt catcgaagct gagacgcacc ctgcaggtgc
      361 tcgtcgatcg tcacgcgtgc ctgaggaccc gattcgagaa ccggaacgga cgtccgtggg
      421 ccgatgtccg agagcgcgac gaggtgagtt tcacggaagt ggacgccgcc ggttggactg
      481 acgcgcgcct gcagcagtat ctgaccgagc agagccacag gccgttcgac ctcgcgcgcg
      541 gccccctctt caccgtccat ctgttgaccc gttccgcgcg tgatcacgtc ctgttgattt
      601 gcgtccacca catctcggtg gacttctggt cgctcgtcgt gctgaccgac gagatgggcc
      661 gtctgtatgg ggctgaagac ccgcaggcgg aagcgaagca gctgcctctt ccggcgccgt
      721 acgccgagtt cgtccggtgg caggacggga tgctcgggag cgacccgggc cgggagcacg
      781 aaacctactg gttgaaacag ctctccggga cgctcccggt cctcaacctt ccgaccgatc
      841 ggcctcgaac gccggtacag acctaccgag gcgcctccca cacgtttcaa ctgggcccgg
      901 aactgacccg ggcgttgcac gacctcgccc ggcagcagca ggtcactctc tattcgctgc
      961 tgctcgcgac attccaatcg ttgctgcatc ggtacaccgg cgatgcggat gtgttggtcg
     1021 gctcgcccgt ggcggggcga agccgggagg gattcggcgg cgtcgtgggc tacctcgtca
     1081 acatggtcgc cctgcgtggc gatctatcgg acgatccgac gttcaccgag cttctgtccc
     1141 gcacgcgcgc gattgttctg ggagcgctgg cgcaccagga ctttcctttc gctctcctcg
     1201 ttgatcgtct cgaacagaat cgggacccga gtcggtcgcc cgtctttcag gccgcctttg
     1261 ttctcgaaaa gccgcacatc cgggaagagc tggccgagtt cgtcctggga gagagcggcg
     1321 cgaccatgtc tctgggcgga ctccagctgg agtctctggc gctcgagcag cgggttgcgc
     1381 agttcgacct gacgttgatg atggtcgaag ccggcggcgg tctcgccgcg tccatccagt
     1441 acaacgccga cctgttcgat ccctcgacga tctcccgcct cgctcgacac taccagacgc
     1501 tggtcgaggg cgtttccgcc gatccccagc gccgcctgtc cgagctgccg cttctgtcgg
     1561 ttgaggaacg tcggcagctc ctcgaatgga agggagaggt cgcggacgtc tcaccggctg
     1621 tgacgctgca gggactgttt gaagaacagg tcgcgcggac tcccgatgcg gtgtctgtcg
     1681 cctgggatgg cgggcaactg agctaccgcg agctgaatcg ccgtgccaat cagctcgcgc
     1741 accacctcag gagacgcggc gtcggtccgg aaaccctggt gggcgtctgc atggatcgct
     1801 cgccggagat gatcgttgcg atcctcggca tcctcaaagc aggcggcggc tacttcccgc
     1861 tggatcccgg ctatccacgg gaccggctgt cgttcatgct tcgcgacggc gacgtgcggc
     1921 tggtgctcgg caaggaccag ttggccgagg cctcaggcga gagcaccgag aatcccgtcc
     1981 cgttgtctac ggggcagaac ctcgcgtacg tgatgtacac gtcaggctct actggcactc
     2041 cgaacggcgt cgccgtcagt cagcagtctg tgatccgcct cgtggccgac cccaactacg
     2101 tcacgctgac acccaacgaa gttgtcctgc agctcgctcc caacacgttc gatgccgcga
     2161 cgttcgaagt ctgggcaagc ctgctgcacg gtggaagact cgtgctcgcg accgacgaca
     2221 agccgtcgct ggagcaggtc agccagatgc tcgcgaaaca ccgcgtgtcg acgctctggc
     2281 tgacgtcggg tctgttccac cagctcgtgg atcacgacat cgacgccttc ggcaccgttc
     2341 gtcagctcct ggccggcggc gacgtgctgt cggtcagcca cgtgcggaac gtgctgacgc
     2401 gattcccgtc gtgccgcgtg atcaacgggt acgggccgac agagaacacg acgttcacct
     2461 gctgctatcc catgcagggg gacaaggccg cgcctgcggt ctctgtgccg atcgggcgcc
     2521 cgatcaccgg gaccgaagtg ttcgtggtgg accggcatca gcacgcggtg cccgtcggag
     2581 tgtccggaga actgctgacc ggagggaccg ggctggcgcg cgggtacctc ggccgtccat
     2641 cgctgacggc agagagattc gttccgagca cgctcagcga acacggaggg gctcgtctct
     2701 acaagacggg cgatctggtt cgctatctgg
//
LOCUS       DBNGSH010000354         4536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2746148, whole genome shotgun sequence.
ACCESSION   DBNGSH010000354 DBNGSH010000000
VERSION     DBNGSH010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2746148"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="AC16CY_08335"
     CDS             complement(<1..657)
                     /locus_tag="AC16CY_08335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3353833.1"
                     /translation="MRVAIDTRKVHDFGIGTYIRNLLRQLARIDRETEYVLLCREQDL
                     GIAAQLGPNFRTIREPSPNYSLREQIHIPWVLRRERPDVYHAPHYVLPPAVFCRSVVT
                     IHDCIHLMFPQYLPNRAAFAYARASMWAAVHRSDRILTVSAASKRDILRLFDVPPEKV
                     VVVYNAIDEHFSTEPSPEHISRVRERYQLDHQFVLYVGNIKPHKNLVRLIEAFSRLRQ
                     D"
     gene            complement(734..2110)
                     /locus_tag="AC16CY_08340"
     CDS             complement(734..2110)
                     /locus_tag="AC16CY_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3353834.1"
                     /translation="MDASSIAAWNCPSCRRRVPRQVEECRCGFKWADLPPVDTTPAGP
                     PAAPSNKRGLILLAAGLVIGGGLMLLVLRPAPAVNVATANTPTPAAPTENALATPPGG
                     GEPVLTTSGALLLTTPTTPPAETAVPVTPPPAASLEEVVSQVVPAVASIVAGQSRGTG
                     FFVRPDQVLTNAHVVEGQTSVRLQVGDTSYTARVATIAAGSDLAVLQVYNPNPKQPTL
                     RLGSATRARVGEEVIAVGSALGVLSNTVTRGIVSAIRDVGPVRLLQTDAAINPGNSGG
                     PLVDRTGQVIGVNSMVVAAREGQGLAFAVAIDHAVPLLNGKGAGAAAQTPLNALSQAM
                     GGPGAGEEMRARGEQAYEQVLAWAAQTSQQLDAYWDRYASTCVTSSNRSGDRAWFAVL
                     EPNGVRINPAAAINCQSWLETVQSNALPIKATIDQAADAARRSGVYPGVLRDMRRKHR
                     LDWTGWER"
     gene            complement(2145..2972)
                     /locus_tag="AC16CY_08345"
     CDS             complement(2145..2972)
                     /locus_tag="AC16CY_08345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3353835.1"
                     /translation="MTRLTIVIVGYNSKDDLARVLPSLTSPPPAVDHEIVVVDNASTD
                     STAAFVRASWPGVRLIVSDTNLGFAKANNLGVRATASDLVLFLNPDTVVPPGVVDRLV
                     SALDARPDVAIVGPRIVDGRGQAELSFGALVTPWAELKQKVLVRGNDLGVPLVTALVD
                     RMTRRTRTVDWVSGACLLIRRSDLEAAGGFDERFFMYIEDVDLCASVRARGRLVLFAA
                     EPEVVHLRGRSAASAVRETSQTYRRSQIAFYAKHHPAWVPVLEAYLKIRGRWPDTSQ"
     gene            complement(2969..4036)
                     /locus_tag="AC16CY_08350"
     CDS             complement(2969..4036)
                     /locus_tag="AC16CY_08350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3353836.1"
                     /translation="MRIAIDGRELVGKPTGVGRYLSELLAAWSDLPGATAHEFFLCTP
                     EPVSARRTGALRLSQVVSPGGGTLWEQWTLPRLVSATGADVLFSPAYTCPLRCPAPIA
                     LVIHDVSFAAHPEWYSRRDGFRRRTLTWLAARRAARVITDSDFSKREIARHLGIDPSR
                     IDVIYLGASAPAAVSSRDSLVLFVGSLFNRRHIPELIEGFTRLNARHPRVRLEIVGDN
                     RTMPRLDVDALIAERGAAGAVRARSYVTDAELATLYATASAFAFLSEYEGFGLTPLEA
                     MAAGVPVVVLDTEIAREIYGPAAVYLERPDPDLIASALERLLVDERERARLAEAGALH
                     VERYSWRECGQRTLQVLLATT"
     gene            complement(4040..>4536)
                     /locus_tag="AC16CY_08355"
     CDS             complement(4040..>4536)
                     /locus_tag="AC16CY_08355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3353837.1"
                     /translation="DAYVRLLDTARRVPRLLIAGGATPAAAPWLDRIARDPLKRVVSY
                     AGYVDNADRERLYAGARAVVLPSLDEGFGLTVLEAMSAGIPVLASNRGSLPEVIGPEG
                     VLLDPHDSTAWAAAIERVSADAQWAESLGRTGLERARTFAWKETAARVHTAYTDAVAR
                     RRER"
BASE COUNT          711 a         1540 c         1595 g          690 t
ORIGIN      
        1 gtcctggcgc agccggctga atgcctcgat gagtcgcacg aggttcttgt gcggcttgat
       61 attgccgacg tacagcacga actggtgatc gagctggtac cgctcgcgca cccgcgagat
      121 gtgttcgggg gacggctcgg tgctgaagtg ctcgtcgatg gcgttgtaga cgacgacgac
      181 cttttcgggc ggaacgtcga agaggcgcag gatgtcccgc ttcgacgcgg ccgacaccgt
      241 caggatgcga tccgaccggt gcacggcggc ccacatcgac gcgcgcgcgt acgcgaaggc
      301 ggcgcggttg ggcaggtact gcggaaacat caggtggatg cagtcgtgga tcgtgaccac
      361 ggagcggcag aacaccgcgg gcggcagcac gtagtgcggg gcgtggtaga cgtcggggcg
      421 ctcccgccgc agcacccacg ggatgtggat ctgttcgcgc agcgagtagt tgggcgacgg
      481 ctcgcggatg gtcctgaagt tggggccgag ctgcgcggcg attcccagat cctgctcacg
      541 gcagaggagg acgtactccg tctcgcgatc gatccgcgcg agctggcgca ggagattgcg
      601 gatgtaggtc ccgatcccga aatcgtggac tttccgcgtg tcgatcgcga cgcgcatacc
      661 gcgtcattat atcggtgcga acctgatacc gtcggtcgtc agggacgtcg caccgaccag
      721 cgcagggagg ctctcaccgt tcccagccgg tccagtccag tcgatgcttg cgacgcatgt
      781 cccgcaggac gcccgggtag acgccgctgc gccgagcggc atcggcagcc tgatcgatcg
      841 tggccttgat tggcagcgcg ttggactgca cggtctcgag ccagctctgg cagttgatcg
      901 ccgccgccgg gttgatgcgt acgccgttcg gttcgagcac cgcgaaccac gcgcggtcgc
      961 cggacctgtt ggacgatgtc acgcaggtgc tcgcgtagcg atcccagtag gcgtcgagct
     1021 gctggctggt ctgtgcggcc cacgcgagga cctgttcata cgcctgctcg cctcgcgcac
     1081 gcatttcctc gcccgccccc ggtcctccca tcgcctgact caaggcattc agcggcgtct
     1141 gggctgccgc ccccgcgccc ttgccgttca gcaacggcac ggcgtgatcg atcgcgacag
     1201 cgaacgccag gccctgacct tcgcgtgcgg ccaccaccat cgagttgacc ccgatcacct
     1261 gtcccgtccg atccaccagc ggtccgccgc tgttgccagg attgatcgcc gcgtcggtct
     1321 ggagcagccg cacggggccg acgtcccgga tcgcgctgac aatgcctcgc gtgacggtgt
     1381 tggacaggac gccaagagcc gacccgacgg cgatgacttc ctctccgact cgcgccctcg
     1441 ttgccgatcc gaggcggagc gtcggctgtt tcgggttcgg attgtagacc tgcaggacgg
     1501 cgaggtcgct cccggcggcg atcgtcgcga cgcgagcggt gtagctcgtg tcgccgacct
     1561 gcagtcgcac ggacgtctgt ccctcgacga cgtgagcgtt ggtcaacacc tgatcgggtc
     1621 ggacgaagaa cccggtgccg cgcgactggc ccgcgacaat cgacgccacg gccggcacca
     1681 cctggctcac gacctcctcg agcgacgctg ccggcggcgg cgtgaccggc accgcggtct
     1741 cggccggcgg ggtcgtcggc gtcgtgagga gtagagcgcc ggacgtggtc aacacgggtt
     1801 cgccgccacc gggtggcgtc gcgagggcgt tctcggttgg cgccgccggc gtcggcgtgt
     1861 tggccgtcgc gacgttgacg gcaggggcag gccgcagcac gagcagcatc agtccaccgc
     1921 cgatcacgag gccggccgca agcaggatga gtccccgctt attcgacggt gccgcgggcg
     1981 gcccggcggg agtcgtgtct acgggtggca gatcggccca cttgaacccg catcggcact
     2041 cctcgacctg acgcggcaca cgccggcggc aggacgggca attccaggca gcaatcgatg
     2101 acgcgtccat gttcttcggc cggtgcgccc gggcagactc ggccttactg ggatgtatcg
     2161 ggccaacgcc cccgaatctt gaggtaagcc tcgagcaccg gcacccaggc cggatggtgc
     2221 ttcgcgtaga aggcaatctg gctgcggcgg taggtctggc tggtttcccg gacggctgat
     2281 gccgccgagc gtcccctcag atgtacgacc tccggctctg cggcaaagag gaccagccgg
     2341 ccgcgcgcgc gcacggacgc gcagaggtcc acgtcctcga tgtacatgaa gaatcgctcg
     2401 tcgaatcctc cggcggcctc caggtcggag cggcgaatca gcaggcacgc gccgctcacc
     2461 cagtccaccg tgcgcgtgcg gcgcgtcatc cgatcgacga gcgccgtgac gagcggcacg
     2521 cccaggtcgt tccctcggac gagcactttc tgcttgagct cggcccatgg cgtgacgagc
     2581 gcgccgaagg acagctccgc ctgtccgcgc ccgtcaacga tgcgaggtcc cacgatggcg
     2641 acgtcgggcc gcgcgtcgag cgccgacacc aggcggtcga cgacgcccgg cgggacgacg
     2701 gtatccggat tcaggaagag cacgaggtcg ctcgccgtgg cgcgcacgcc gaggttattg
     2761 gccttggcaa accccaggtt cgtgtccgag acgatcaggc gcacgcccgg ccacgacgcg
     2821 cgtacgaacg cagccgtcga gtcggtcgat gcgttgtcca cgaccacgat ctcgtggtcc
     2881 acggccggcg gcggactggt cagcgatggc agtaccctcg cgagatcgtc cttcgagttg
     2941 tagccgacga tgacaatggt gagccgcgtc atgtcgtggc gagcaggacc tggagcgtgc
     3001 gctggccgca ctcgcgccat gaatagcgtt ccacgtggag cgcgccggct tcggcgagtc
     3061 gggcgcgctc gcgttcgtcc acgagcagcc gctcgagcgc cgacgcgatg agatcgggat
     3121 ccggccgctc caggtacacg gcggccggcc cgtagatctc gcgcgcgatc tcggtgtcga
     3181 gcaccacgac tggaacgccg gccgccatcg cttcgagcgg cgtcagcccg aatccctcgt
     3241 actcggacag gaaggcgaag gcggacgccg tcgcatacag ggtcgccagc tcggcgtcgg
     3301 tgacgtagga tcgcgcgcgc acggccccgg cggctccgcg ctccgcgatg agggcgtcca
     3361 cgtcgaggcg cggcatggtg cgattgtcgc cgacgatctc aagtctcact cgcggatgcc
     3421 gcgcgttcag gcgcgtgaac ccctcgatga gctcggggat gtgccgccgg ttgaagagcg
     3481 agccgacaaa cagtaccagg gagtctcgtg aagacaccgc ggccggggcg gacgcgccca
     3541 ggtagatcac gtcgattcga gacgggtcga tcccgaggtg cctggcgatc tcgcgcttcg
     3601 agaagtccga atcggtgatg acgcgcgccg cacgccgggc cgccagccac gtcagcgtgc
     3661 gccggcggaa gccgtcgcgc cgcgaatacc actcaggatg cgcggcgaac gagacgtcgt
     3721 ggatcacgag cgcgatcggc gcggggcatc tgagcgggca cgtataggct ggcgagaaca
     3781 gcacgtcagc acccgtggcg gagacgagcc ggggcagggt ccactgttcc cacagcgtgc
     3841 ccccgcccgg cgagacgacc tgcgacagac gaagagcgcc cgtgcggcga gctgagaccg
     3901 gttcgggcgt gcacagaaag aactcgtgcg ccgtggcgcc gggaaggtcg ctccatgccg
     3961 cgagcagttc cgacaggtag cgtccaacgc cggtaggctt tcccacgagc tctcgcccgt
     4021 cgatggcgat tcgcatccgc taccgctccc tgcgtctcgc gacggcgtcc gtgtacgccg
     4081 tgtggacccg ggccgcggtc tccttccacg cgaacgtccg ggcgcgctcg agcccggtcc
     4141 gtcccaggct ctcggcccac tgcgcgtccg ccgacacgcg ctcgatcgcg gcggcccagg
     4201 cggtgctgtc gtgcggatcg aggagcacgc cttccgggcc gatcacctcg ggcagcgatc
     4261 cccggttcga ggcaagcacg gggattccgg ccgacatcgc ctcgagcacg gtgaggccga
     4321 acccctcgtc gagagacggc agcacgacgg cccgcgcgcc ggcgtacagc cgctcgcgat
     4381 cggcgttgtc gacgtatccg gcgtaggaca cgacgcgctt gagcgggtct cgcgcgatgc
     4441 gatcgagcca cggggccgcg gccggcgtgg cgcctccggc aatcagcagg cgaggcacgc
     4501 gccgcgccgt gtccagcagc cgcacgtagg cgtcga
//
LOCUS       DBNGSH010000355         4873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3595089, whole genome shotgun sequence.
ACCESSION   DBNGSH010000355 DBNGSH010000000
VERSION     DBNGSH010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3595089"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="AC16CY_08360"
     CDS             <1..334
                     /locus_tag="AC16CY_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3353838.1"
                     /translation="AGSGFCGMNINPVSVRGGGVTMSALAGLLVAATERLVIDRTGLT
                     GNWDLDVKYTPDRSQIPPGVEPPVPIDWNGPSLFTALEEQLGLKLRPARGPVEVLVID
                     SVQQPTPN"
     gene            542..1249
                     /locus_tag="AC16CY_08365"
     CDS             542..1249
                     /locus_tag="AC16CY_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HIR3353839.1"
                     /translation="MPNSFTTRFIRQEPAAAQTSSFHFEKPSGFQFTAGQYIDLMLPL
                     VDVPERDRVHTFSISSAPYEDHIAVTTRLRDSPFKRTLRGLTPGDAVEVEGPYGTFVV
                     SRDTPRPVAFVIGGVGITPAISIIKQAARDAELADIYLFYFNRSQAEAPFLAPLTRLQ
                     SEHAGFHLIAAMTDEPSWNGEKERVSIDMLRRYLDPLSTSFFMSGPPGMVTAVRELLA
                     SNGVPKAQIFFENFAGY"
     gene            1252..4464
                     /locus_tag="AC16CY_08370"
     CDS             1252..4464
                     /locus_tag="AC16CY_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3353840.1"
                     /translation="MGPLNAASSPLCSLGLWAGRVTGAIVGRPREVAAIEEELAAVDR
                     GLTCLTLEGEPGIGKTRLLLAVDAMARARGFVSIAVTADEEIRGPFLLARSIFACPRI
                     EEAHASDAREAIDRLTGALTDRDDPALASLPPDQKLLRIFDLAALALRLLANERPVVV
                     IVDDLQWVDGDSLRLLRYVARADASSRILLVVAIRPSEVAFVNEAVTLLADLERMGLV
                     RRLKLKRFTQSQSAEFLQQAFAGPIQPASAAVMHAQAEGVPFVLSEQAHVYRDSGLIR
                     QIDGVWTLAPNAERLLPSAVRTLIERRAVRLPEKTKIVLAEAGVLGRMFSLRDLAEVR
                     HRLGESDGLGELAEALEPAVAAGLVIEQPKDSPADYSFTHDQIRESLVVGLASSRQRA
                     IHGAIVDILMGGSGEPLRESLSLLSQHALAAGRTDLCLRFSIEAARAALEAKAPEEVL
                     SLVHRALPVASAAQDRVRLLCLQDDAFNMLRRQDQRLETLSELGALAEALADPHLAHD
                     VQLRRAAALRLSRDEESAAEIARRVLESAARQGDDAAELAACLELGQDLLKSELGAGF
                     VSSPTETDLEGAEQAYTRATVLAARLPDDSMLAAASRELGTIAVSRLRMRFISAMESG
                     AYVPLLQRVASGEHFGDILPTLPGVSERVAEAKGWFQRALEIYERLGDRQGAMTTIIA
                     MAIASWGPEIHVAGSAKRIEEIRRLMARLKSLTKESDRALANAQMLFGSHVYAMAKGM
                     PDVAIAKGEEAYRAARGIGERSLEFAAAGRTALANAHVDRKTEARKWLDLAAAVVTAS
                     PTPGRARRIELWRGAVLARAGDAAGMQQHLEHSARLAAEQDLPAARCEALALLAIEAA
                     RLGRARSDDALLSLAERSAEDVLRECSSLPGHPQWPSQALAARATVALARNDLKIAVE
                     FAWQALDHHDAAKREDLDLEIILPAADAIIAGGSKEEASAVRGRLQMLLGFQSQRIQD
                     EQVRAEWFRSETGRCLTRLAGSLAAVRPQGSGAAESGLAADDSRLLGLLVEGKTNREI
                     AHEIDADEQAVTMRLAQLFATIGASSRADATVAALVGGLI"
     gene            4483..>4873
                     /locus_tag="AC16CY_08375"
     CDS             4483..>4873
                     /locus_tag="AC16CY_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR3353841.1"
                     /translation="MLHVRIDRHRCIGVGNCITLAPTAFDWFKGDYFKAAVVDLTSID
                     EDTLREAVLSCPTQAIVLEEIEELLPWQLRGSQEAGSVRVARTFVFTDIVRSTNLVEV
                     LGDEAWAALLRWHNETLRSVFIAHQGTE"
BASE COUNT          814 a         1587 c         1623 g          849 t
ORIGIN      
        1 ggcggggtct ggcttctgtg ggatgaacat caaccctgtc agcgtcaggg gcggcggggt
       61 gactatgtcg gcgctggcag gccttctcgt ggcggccacg gagcgcctgg tgatcgatcg
      121 caccggattg acgggcaact gggacctcga tgttaagtac acacccgatc ggtcgcagat
      181 tcccccgggc gtcgagccgc cagtgccgat cgattggaac ggcccgtcgc tgttcaccgc
      241 gctcgaggaa cagctcgggc tgaagctgcg tcccgcgcgc gggccggttg aagtgctcgt
      301 catcgactcg gttcagcagc ccacaccgaa ctgaactgag atgtcgggag tcgggcggcc
      361 ccgcctgaga tggccatctg tctacatgac agtgaccagt tcgtcggcgg ccgttgcttc
      421 gagagatgcg gccatcgtca tcccttgcct gacgccacgg tccgcgagat atcatgccca
      481 tcatggattc gagccgcacg tcagcgctga cacctgacgt gcgcccatca gggaacacat
      541 cttgccgaac agcttcacca cccgcttcat ccgccaggaa ccggcggctg cgcagacgag
      601 cagcttccac ttcgagaagc catcaggatt tcagttcacg gccggccagt acatcgacct
      661 gatgttgccg ctggtcgatg tgccagagag ggacagagta cacaccttct cgatctcgag
      721 cgcaccctac gaggaccaca tcgccgttac caccaggctg cgagacagcc ccttcaagcg
      781 gaccttgcgc ggcctgacgc cgggtgacgc cgtcgaagtc gagggcccgt acggcacgtt
      841 cgtggtctcg cgggacacac cccggcctgt cgccttcgtc atcggcgggg tcggcattac
      901 acccgcgatc agtatcatca agcaggccgc ccgggacgcc gagctggccg atatctatct
      961 gttctatttc aacagaagcc aggcggaggc gccgtttctc gcgccattga cccgccttca
     1021 gtcggagcat gcaggattcc atctcatcgc cgccatgacc gacgagccgt cctggaacgg
     1081 tgagaaggaa cgcgtcagca tcgacatgct ccgccggtac ctcgacccgc tcagcacgtc
     1141 cttcttcatg tcgggaccgc ccggcatggt gaccgccgtc cgagagctgc tggcgtcgaa
     1201 cggcgttccg aaagctcaaa tcttcttcga aaactttgca ggctactgag agtgggtccg
     1261 ctgaatgccg cctcttcgcc gctctgctcg ctcggcctct gggctggccg cgtcacgggc
     1321 gccatcgtcg gccggccgcg cgaagtcgct gcgattgaag aggagctggc cgctgtcgat
     1381 cgcgggctca cctgtctgac cctcgaaggc gagccaggca tcggcaagac gcgcctgctg
     1441 ctggcggttg acgccatggc acgcgcgcga ggcttcgtgt cgattgcggt gacggccgac
     1501 gaggagatcc gggggccgtt tctcctggca cgcagcatct tcgcctgccc gagaatcgaa
     1561 gaggcgcacg cgagcgatgc tcgagaggcc atcgatcggt tgaccggtgc attgaccgac
     1621 cgggacgatc cggcactggc cagcctcccg cccgatcaga agcttctgcg catcttcgat
     1681 cttgcggcgt tggcgctccg gctgctggca aatgagcgtc ccgtcgtcgt gatcgtcgac
     1741 gacctgcagt gggtggacgg ggacagcctt cgactgctcc gctacgtggc gcgcgccgac
     1801 gcctcgagtc gaatcctgct cgtggtcgcg atcaggccgt cggaagtggc gtttgtcaac
     1861 gaggccgtca cgctccttgc ggacctggaa cgaatggggc tggttcggcg gctgaagctg
     1921 aagcgattca cccagtcgca gtcggccgag ttccttcagc aggcgttcgc cggtccaatc
     1981 cagcccgcca gtgccgccgt catgcacgct caggcggaag gcgtgccgtt cgtgctgtcc
     2041 gagcaggcac acgtctatcg cgactcgggc cttatccggc agatcgacgg cgtgtggacg
     2101 ctcgcgccga acgcggagcg gctgcttccg tcggccgtcc gaacgctcat cgagcgtcgg
     2161 gccgtgcgtc tcccggagaa gacgaagatt gtcctcgcgg aggcaggggt tctgggacgc
     2221 atgttcagcc tccgagacct cgcagaagtc aggcaccggc tcggtgaaag cgacggcctc
     2281 ggcgagctgg ccgaggccct ggagccggcg gtcgccgccg ggctcgtgat cgagcagcca
     2341 aaggattcgc ccgccgacta cagcttcacg cacgatcaga tccgagaatc gctggttgtc
     2401 gggctcgcgt catcccgcca gcgggccatc cacggagcca tcgtcgacat cctgatgggt
     2461 ggcagcggcg agccgttgag agaaagcttg tctctcctca gccagcacgc gttggcggcg
     2521 gggcgaaccg acctctgttt gcgtttttca atcgaagcgg cgcgagcggc tctggaggcc
     2581 aaggccccgg aggaggttct gagcctcgtt catcgagccc tgcccgtggc atcggctgcg
     2641 caggaccggg tgcgtctgct ctgcctgcag gatgacgcct tcaacatgct gcgccggcag
     2701 gatcagcgcc tggaaacgct ctccgagctc ggagccctgg ccgaggcgtt ggccgaccct
     2761 cacctggcgc atgatgtcca gctgcgccgt gcggcggcac tgcgcttgtc gcgcgatgaa
     2821 gagagcgcag ccgagatcgc gcgcagggtt ctcgagtccg ccgccaggca gggtgacgac
     2881 gcggcggagc tggcggcgtg cctcgaactc gggcaggact tgctcaagtc cgagctgggt
     2941 gccggattcg tttcctcacc taccgagaca gacctggaag gcgccgagca ggcgtacacg
     3001 cgggcgactg tcctagccgc gcgattgccc gatgactcga tgctcgcggc agccagccgg
     3061 gaactcggta ccattgccgt gtcccgtctt cgtatgcggt tcatctccgc catggagtcc
     3121 ggcgcgtacg ttcccctctt gcagcgggtt gcgtccggcg agcacttcgg cgacatcctc
     3181 ccaaccctcc ccggcgtttc cgaacgcgtg gccgaggcca aaggctggtt ccagcgcgca
     3241 ttggagatct atgagcgcct tggggaccgc cagggcgcca tgacgacgat catcgcgatg
     3301 gcgatcgcca gttgggggcc cgagatccat gtcgcgggct cagccaagcg aatcgaggag
     3361 attcgccgtt tgatggcgag actgaaatcg ctgaccaagg agagcgatcg tgcgctcgca
     3421 aacgcacaga tgctgtttgg atcgcatgtc tacgcgatgg ccaaaggcat gcccgacgtc
     3481 gccatcgcca agggtgagga agcgtaccgc gcggcgcgtg ggatcggtga acggtcgctc
     3541 gagttcgccg cagcgggccg gacggccctc gccaacgcgc acgtggatcg gaagactgaa
     3601 gccaggaaat ggttggatct cgccgcggcc gtggtcaccg cttcgccgac gccgggacga
     3661 gcacgccgga ttgaactctg gcgcggcgcc gttctggccc gagcgggcga cgccgccggc
     3721 atgcagcagc atctcgaaca ctccgcccgg ctggccgccg aacaggatct tccggcggcg
     3781 cgctgcgagg cgctggcgct cctcgcgatc gaagcggctc gacttggacg cgcgcgtagc
     3841 gacgacgcgt tgctgtcgct cgcggaacgg tccgccgagg atgtgttgcg cgagtgctcg
     3901 tccttgccgg gccaccctca gtggccgagc caggcgctcg cggccagggc gaccgtcgcc
     3961 ctggccagaa acgatctgaa gatcgccgtg gaattcgcct ggcaggcgct cgaccatcac
     4021 gacgccgcga aacgggaaga tctggatctc gagatcatcc tgccggccgc cgatgccatc
     4081 atcgccggcg ggagcaagga agaggccagc gctgtgcgcg gacggctcca gatgctgctc
     4141 ggattccaat cgcaacgcat ccaggacgaa caggtgcggg ccgaatggtt ccgcagcgag
     4201 acgggccgat gcctgactcg cctggctggc tcccttgccg cggtgcgtcc ccagggttcc
     4261 ggcgctgcgg agagcggtct ggcggccgac gattcacggc tgctggggct gctcgtcgaa
     4321 ggcaagacca atcgtgagat cgcccacgag atcgacgccg acgagcaggc ggtcacgatg
     4381 cgtcttgcac aactgtttgc cacgatcggc gcctcgtccc gagccgacgc cacggtggcg
     4441 gcgctcgtcg gaggactgat ctaaccccgc tgtggtgtgg tcatgcttca tgtgagaatc
     4501 gatcgtcatc gctgcatcgg cgtcgggaac tgcatcaccc ttgcgccgac ggcgtttgac
     4561 tggttcaaag gggattactt caaggccgct gttgtcgacc tgaccagcat cgatgaggac
     4621 acgctgaggg aggctgtcct ctcatgtccc actcaggcca tcgtcctcga ggagatcgag
     4681 gagctcctgc cctggcagct tcgtggcagc caggaggccg gctccgtccg ggtggccagg
     4741 acgttcgtgt tcaccgacat cgtgaggtcg acgaacctgg tcgaggtact gggtgacgag
     4801 gcgtgggccg cgctgctgcg ctggcacaac gaaaccctgc gttcggtgtt catcgctcac
     4861 cagggaaccg aag
//
LOCUS       DBNGSH010000356         2964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_199658, whole genome shotgun sequence.
ACCESSION   DBNGSH010000356 DBNGSH010000000
VERSION     DBNGSH010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_199658"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="AC16CY_08380"
     CDS             <1..825
                     /locus_tag="AC16CY_08380"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3353842.1"
                     /translation="IAWLLVEPLIKDRDPGHIAHARDVMRAMALFPGVVDRYLRSRLW
                     WRRALAVRTCGVLQITNRTAPIVAALDDANPDVRGAALDALTDLRDPASLQAVVVRMH
                     DASLHRGRRLAALASFGHLAETFVLDLAEVNPEHRVNYARALALCGSERSRPVLCEWT
                     ADARADVRAAALEALAHVGLDDHSATLAVRALDSPDALVRSMAARALRGWEGPGEAAS
                     HLARHLDDTWVVAVQAARSLQQMPRTGLMELQTLATRSDLAGLLARQMLWEASAQY"
     gene            825..2237
                     /locus_tag="AC16CY_08385"
     CDS             825..2237
                     /locus_tag="AC16CY_08385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3353843.1"
                     /translation="MATSLLIALSLLVTAYLVIWHVSQMAMGPVGALFLWRHRRRHTR
                     RARALVGDLASPPLVSVVVPALNEELTIVESVRAMLALDYESREIVVVNDGSTDGTLE
                     LLQRTFGLLPAPLAFDQPIPTAPVRGIYRSIREPGLVVIDKENGRCKSDASNAGINAA
                     SGVLVLVIDADTVLEPDALSRAVLPFLEDPTTVAVGGNIAIVNGSRIEHGRVAEVSLP
                     RSWLARFQIVEYMRAFLLFRLACASVNGVLLISGAFGLFRRDAVIAVGGFDHKAIGED
                     MDLTVRLQRHHRARRLPFRITFDPNPLAATQAPEDWRSLKSQRCRWRRGLLQVLWRHR
                     RMIGNPRYGVVGLALLPYTAFIDGLGPLIEISGYVITTVGAVSGFLDWHYYGVLIFVS
                     VTLGAASTFQAILLNDVATQRYMRGRDLLLLMAVSLLENCGYRQLNAWWAVVGTVQAM
                     TGKSGWGPMKRQSFQGEETA"
     gene            2354..2581
                     /locus_tag="AC16CY_08390"
     CDS             2354..2581
                     /locus_tag="AC16CY_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353844.1"
                     /translation="MKRQLTFGLVLLLAGLAVLATPMMRYTTTQKAFDIGPVEIVTEK
                     EKQVSLPQTLGVVSAAAGIALIATSRRHAAA"
BASE COUNT          447 a         1005 c         1002 g          510 t
ORIGIN      
        1 atcgcctggc tgctggtgga gccgctcatc aaggatcgcg atcccggtca tatcgcgcac
       61 gcacgcgacg tcatgcgcgc gatggcgctg ttcccgggtg tggtcgatcg ctacttgagg
      121 agccgcctgt ggtggcgtcg tgcgctcgcg gtgcggacgt gcggagtgct gcagatcacg
      181 aaccgcaccg cgcccattgt cgctgccctg gatgacgcga accccgacgt ccgcggcgcg
      241 gcgctcgacg cgctcacgga tctccgcgat cccgcgtcgc tgcaggcagt cgtcgtccgg
      301 atgcacgacg cgtccttgca taggggccgg cgcctggcgg cgctcgcatc gttcggtcac
      361 ctcgccgaga cgttcgtgct ggacctggcg gaggtgaatc cggagcaccg agtcaactat
      421 gcgcgggcgc tcgcgctgtg cggctccgaa cggtcgcggc cggttctgtg cgagtggacg
      481 gctgacgcgc gggccgacgt ccgcgcggcg gcgctcgagg cgctggcgca cgtgggactc
      541 gacgaccatt ccgcaaccct ggccgtccgc gcgctcgaca gccccgatgc actcgtgcgt
      601 tcgatggcgg cccgcgccct gcgcggctgg gaggggccgg gcgaggccgc gtcgcacctc
      661 gcgcgccatc tcgacgacac gtgggtggtg gcggttcagg ccgcgcggtc gctgcaacag
      721 atgccgcgta caggcctgat ggaactgcag accctggcca ctcgctccga cctcgccggc
      781 ctgctggcgc gccagatgct gtgggaggcg agcgcgcaat actgatggcc acttcgttgc
      841 tgatcgcgct gtctctgctg gtcacggcgt acctcgtgat ctggcacgtg tcgcagatgg
      901 ccatgggccc ggtcggggcg ctctttctct ggcggcaccg gcggcggcac acgcgccgcg
      961 cgcgcgcgct cgtcggcgac ctcgcgtcgc cgcccctcgt gtcggtcgtc gtgccggccc
     1021 tcaacgagga gctcacgatt gtcgagagcg tgcgggcgat gctcgcgctc gactacgagt
     1081 ctcgcgaaat cgtggttgtc aacgacggat cgaccgacgg cacgctcgag ttgctgcaac
     1141 ggaccttcgg cctcctgccc gccccgctcg ccttcgatca gccgatcccg accgcgcccg
     1201 tgcgcggcat ctaccggtcg atccgcgagc ccgggctcgt ggtcatcgac aaggagaacg
     1261 gccggtgcaa gtcggatgcg agcaacgccg gcatcaacgc cgcatcgggc gtcctcgtgc
     1321 tcgtcatcga tgccgacacg gtgctcgaac ctgacgcgtt gagccgggcg gtcctgccgt
     1381 tcctcgagga cccgacgacc gtggccgttg gcgggaacat cgcgatcgtc aacggctccc
     1441 ggatcgagca cggccgcgtc gccgaggtgt ccctgccacg cagctggctc gcgcggttcc
     1501 agatcgtcga gtacatgcgg gcgttcctgc tgttccgcct cgcgtgcgcg tcggtgaacg
     1561 gcgtgctgct gatttccggc gcgtttggat tgttccggcg cgacgcggtg atcgccgtcg
     1621 gcggcttcga ccacaaggcg atcggcgagg acatggatct caccgtccgc ctccagcggc
     1681 accaccgcgc caggcgcctg cccttccgga tcacgttcga cccgaacccg ctggccgcga
     1741 cgcaggcgcc cgaggactgg aggtcgctga aatcgcagcg ctgccggtgg cggcgcgggc
     1801 tgctgcaggt gctctggcgc catcgacgga tgatcggcaa cccccgctac ggcgtcgtcg
     1861 gactggcgct gcttccctac accgcgttca tcgacgggct cgggccgttg atcgaaatct
     1921 cgggctacgt catcacgacc gtcggcgcgg tctcgggctt cctcgattgg cactattacg
     1981 gcgttctgat cttcgtgtcg gtcacattgg gcgctgcctc gacgttccag gcgattctgc
     2041 tgaacgacgt cgcgacgcag cgttacatgc gggggcgcga cctgctgctc ctgatggctg
     2101 tctcgctcct cgagaactgc ggctaccgcc aattgaacgc ctggtgggca gtcgtcggaa
     2161 cggttcaggc catgaccggc aagagcggat gggggccgat gaaacgccag tcgttccagg
     2221 gtgaagaaac ggcctgacct cgaccgcaac aggcagctga acccgtcgct tctgcacgaa
     2281 gcgcgaaggc acgaaagatg ctcctcacct ctccacgacg gactctgctg agtcggcgag
     2341 aggagaagtg cccatgaaaa gacagttgac gttcggcctc gtccttctgc tcgcgggatt
     2401 ggccgtcctt gcaacgccga tgatgaggta cacgaccacg cagaaggcgt tcgacatcgg
     2461 ccccgtcgag atcgtcaccg aaaaggagaa acaggtctcg ctgccccaga cgcttggcgt
     2521 cgtgtcggcg gcagccggga tcgcgctcat tgccaccagc cgccgccacg cggctgcatg
     2581 agctggcggc cgtgtaggca ggtagaagtt tacagccgga ccgtgggctg acctgtcaca
     2641 cggtccggct gaagcccgca cgggtccggc tgaagccgga cactacgtac gtcagcggga
     2701 ctgttgcttc gagtaatcga ccaggtctgc tgtggcgacg ccggcctgga cgctggcgct
     2761 ccggtcacca ctctggattt cgacttcttc tttcccaaga ccccgcgcaa cccccagaaa
     2821 ctgaaggccc tcgcgcaaaa taccgtcaaa accggcctgc tgagggccgg gccccggcgg
     2881 ggtggcgtac tatctcgtgc agagctccac gcattgaggt ggcaggacgt gagggatcgc
     2941 agacggagac ttaaccccgg aact
//
LOCUS       DBNGSH010000357        11360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1558368, whole genome shotgun sequence.
ACCESSION   DBNGSH010000357 DBNGSH010000000
VERSION     DBNGSH010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1558368"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="AC16CY_08395"
     CDS             <1..301
                     /locus_tag="AC16CY_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HIR3353845.1"
                     /translation="VALRPVSRQRERSASPIVWTTGYYPSFWGYYGYGWSSVTIGPAR
                     ENTIIVVETLVYDLTRDQLVWAAVSETRNPETLQDFVKDLVKAAVSEMRKAKLVG"
     gene            524..1891
                     /locus_tag="AC16CY_08400"
     CDS             524..1891
                     /locus_tag="AC16CY_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042121 - alginic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HIR3353846.1"
                     /translation="MSTGMRVVAIAVALVAPSVVHAQSAAPSAQNQPEERSSGLPQGV
                     SWTFNFDAGWGSFGFANSLFDNPKDPVPENLSDQWFEGYVKPALSGSYRFASSSEIYG
                     KVSVVGERTYGSAPELYGPDVSSFGPEDLSIGWRSGKSIGSSENLLDFTVGRTQYRLG
                     HGFLLYDGASEGGSRGGYWTNARKAFQFAAIGRVKPGPHLGEVFYLDRDELDEGDSGT
                     RLVGGNYEYSYGENTTIGVTYMKLFAFDGFADPKPHRDGLNVFNVRAFTAPVTALPDL
                     SFEFEYAAERNGDALDSNAWTLQGAYELSKVAWTPKLSYRYALFEGDDPTTAANEAFD
                     PLLLGFYDWGTWWQGEIAGEYFLSNSNLVSHQARVHVQPSDAIGGGVIYFNFLLDQPQ
                     AVAPTVTEKDVANEIDFYTDWKVTGNFTLSFVLAFANPGTAVQQATGRTESFRYGMVY
                     IGYSF"
     gene            1875..3299
                     /locus_tag="AC16CY_08405"
     CDS             1875..3299
                     /locus_tag="AC16CY_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIR3353847.1"
                     /translation="MATASDAPARTFKFPTAYTILFGLIIAVAAATWVVPAGQYDRKK
                     NEQLDKDVPVPGTYKTVEPSPQGLRAVLIAPIAGFYNPDSNQAAAIDVALFVLVIGGF
                     LGVVTKTGAIDAGIGGAMAGLKGNEKWMIPILMALFAAGGTIYGMAEESLAFYALIIP
                     VMIAAGYDALTGVAIIMLGAGIGTLGSTINPFATVIASNAAGVPFTTGMPLRFAILGA
                     GWLAGVVYVMRYAERVRKDPSKSLVADMTKSNREHFLKSRSESGTPVLTGVRSLILVI
                     FGLSFAIMIWGVSSAGWWMAQMSALFLAASILVALIVRMGEEDFTTTFVNGARDLLGV
                     ALVIGIARGIVVIMDAGKITDTILFWSEKAVNGLGTVAFINVMFVLELVLSFFVPSSS
                     GLAVLTMPIMAPLADFSNVGRDVVVTAYQSANGLLNLVNPTFAVVMGGLAIGRVPYNR
                     WLQFTWPLVLVLAVIIVVAISLAA"
     gene            3345..4571
                     /gene="arcA"
                     /locus_tag="AC16CY_08410"
     CDS             3345..4571
                     /gene="arcA"
                     /locus_tag="AC16CY_08410"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037116983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HIR3353848.1"
                     /translation="MQQIGVHSEVGTLRTVLVCRPGLAHQRLTPGNRADLLFDDVLWV
                     HEAQKDHYDFVLKMRERGVEVLDLHELLAETVADKEARTWVLDRRVTANDVGPGMVPI
                     MRPWLDEMAAPKLAEHLIGGIAVLDLPKSEQATMLEEAFGGTDFLVPPVPNTLFQRDP
                     SCWIYNGVTCNPMFWPARQPETLLQRAVYKFHPRFKGANFTIWWGDSDESFGAATVEG
                     GDVMPIGKGVVLIGMGERTTRQAVFQVAAALFKHQGATRVIGCLMPKSRAAMHLDTVF
                     TFCDRDLCTSFREVADQVRCYSARPDGKGGVDVRQDKGHLFDVVKESIGLKTLRVVET
                     GGNAWEAQREQWDDGNNVVALSPGVVVGYDRNTHTNTLLRKAGIEVITVRGSELGRGR
                     GGGHCMTCPISRDPAY"
     gene            4615..5619
                     /locus_tag="AC16CY_08415"
     CDS             4615..5619
                     /locus_tag="AC16CY_08415"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037116981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR3353849.1"
                     /translation="MSFNLRNRSLLTVQDYTTREFRYLLDLARDLKRAKYAKTEQKHL
                     EGKEICLIFEKTSTRTRCAFEVACYDQGAHVTYLDPAGSQIGHKESFKDTARVLGRMY
                     DAIEYRGASQTGVEELAKYAGVPVFNGLTAEYHPTQMLADVLTMREHSDKPIHQIKYA
                     FMGDTRNNMGHSLMIVGCLTGMDVRICGPRKLWPADEYVEIAKKLQEQSGATLTITDD
                     PKKAVAGVDFIHTDVWVSMGEPKEVWKERIDLLLKYQVNMELMKASGNPQVKFMHCLP
                     AFHDTETTLGKQIAQDYGLKNGLEVTNDVFESEYNIAFEQAENRMHTIKAILVATMGD
                     "
     gene            5623..6558
                     /gene="arcC"
                     /locus_tag="AC16CY_08420"
     CDS             5623..6558
                     /gene="arcC"
                     /locus_tag="AC16CY_08420"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR3353850.1"
                     /translation="MLVVTALGGNALLRRGEPMTAENQRRNVRIAAEALAPIAAEPQL
                     VVGHGNGPQVGLLALQGAAYTKVETYPLDVLGAETEGMIGYMIEQELGNLLPFERPFA
                     TLLTMIEVDPGDPAFKDPTKFIGPVYGKEEADRLAAEKSWVFKKDGDKWRRVVPSPEP
                     KRIFELRPIKWLLERNTIVIAAGGGGIPTMYEPGKQRKLAGVECVIDKDFASELLARD
                     LEADLFIMLTDAPAVFVDFGKPTQKAIRRASPAALSAMSFAAGSMGPKVDAACRFAKA
                     TGKKAAIGALADLSKILAGEAGTTVSTKETGIVYA"
     gene            6551..7888
                     /locus_tag="AC16CY_08425"
     CDS             6551..7888
                     /locus_tag="AC16CY_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018896901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR3353851.1"
                     /translation="MPDPPSADRRAGASAPAKSLGLAACTAIVVGNMVGSGFYLSPSA
                     LAPYGLLAILAWIVMGAGAVCLGLTFARLASLAPATGGPYAYSRMGFGDFAGFLVAWG
                     YWISIWASLPAIAVAFTGSLFKLVPNLGGRPVAVAVTLAAMWFVVFTNLRGVKEAGVF
                     AQVTTWSKLVPFAAISIIGLLYVSPENLSQFNPSGQTLLASSAALAPLTMFAFLGLES
                     ATVPAGDVIQPERTIPRSTILGVLIAALLYVLGTTAVMGVVPREQLVKSVAPFADAAQ
                     IMWGTWAAGVVGVAVMISSLGALNGWTLLMGQVPMAAAQDRLFPPLFGKVSPRGVPAV
                     GIVCSAVLATVLVLIQAMAASGFAAFYSLVVNLATMTAVVPYAFCSLAVGLIAVKGSG
                     GRPKRGIGPIEVIAFIFSIFTLYGCGPEAVLYGFVLLMLGIPVFVWQRRASAA"
     gene            complement(7936..8334)
                     /locus_tag="AC16CY_08430"
     CDS             complement(7936..8334)
                     /locus_tag="AC16CY_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353852.1"
                     /translation="MLSFVQEALVRGWENVVGRPDGPMNFRLVMQPAMAIILAVRSGL
                     RDAREGQPPFLWTLLSNRDRRPHLLRHGWNDVRNLFFVAIGLDAIYQVIVHSMVYPLE
                     LLLTATVLALVPYFAVRGLVTRVARRRGRT"
     gene            complement(8340..9392)
                     /locus_tag="AC16CY_08435"
     CDS             complement(8340..9392)
                     /locus_tag="AC16CY_08435"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR3353853.1"
                     /translation="MALARNARFRPRGRATIAVGLVMWLGATLHGQGRTDVVTLRNGD
                     RITGEVVRLERGRLEFETDDAGTLYLEWDKLATVVTTQLVEVITVDSRRFLGSLGQAG
                     PGSIGVASPDGSVTLQMSEVTYITAIGRSFWTKLDGSVDVGFSYTRSSGVEQFNLNWD
                     SVYRKPASQVRLTASATQTSTSDGSSDDRGALEASYLINPWQRWFIVAMGRLETNESL
                     GLELRSQVGAVVGPTLFNGSHGEMAAGGGMVVNHEQGTDVEATQNLEGLLAFRASYYT
                     YDRPKTNLDVSFQYYPSISNFGRQRLQFDTSVRRELLKDFYVSLNLFDTFDSDPPNPD
                     ADRNDVGVVISLGWSY"
     gene            complement(9412..10002)
                     /locus_tag="AC16CY_08440"
     CDS             complement(9412..10002)
                     /locus_tag="AC16CY_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIR3353854.1"
                     /translation="MTVRSQMKRLAGVALLAGACTMWSTEEASAQGFISPLIGYNFSG
                     DSGCPAITNCEDKHLNWGVGFGFLGSIFGVEGEWAYIDDFFGETPTSKSSVTTFMGNF
                     LLAPKFGPVQPYGLIGAGLIKTKVDSTPPLVTDTDQNDFGWDVGGGLMIFFGSHVGIR
                     GDVRYFHSVALLNVFGIETDTKLDFGRASGAVVFKF"
     gene            complement(10098..>11360)
                     /locus_tag="AC16CY_08445"
     CDS             complement(10098..>11360)
                     /locus_tag="AC16CY_08445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3353855.1"
                     /translation="REGDTIARLGGDEFALLLNSLDDEGQANAIAFRIQDALNVPFSI
                     GGREVFTSASIGITFSASQYNNPEEIIRDADSAMYQAKASGKARHEVFDADMRSRALD
                     RLGLENDLRHAVTDTDFEVHYQPIVSLTSGMCVGFEALVRWTRNGVTVAPDKFIPVAE
                     ELGLIEPLGTWVLREACRTFADWQRRFPQNKLECITVNVSTRQLMQQNFLHIVEQAVH
                     EARMTPADLRLEITETTLMGSPFVAADALRQLQDFGVKIYLDDFGTGYSSLSHLHKLP
                     VDALKIDRSFVRSLLLPDRPAIVESILALARTLDTGVVAEGVESDVQARELERLGCRH
                     AQGYYFSPPLSREAAEELLASHRRLGDRGPSTRSRAIETQTEVFFSSAPFEWPDDANP
                     DGAPGAGPRPRGRGDSHGGRVPPASTAA"
BASE COUNT         1946 a         3689 c         3748 g         1977 t
ORIGIN      
        1 cgtggcgctg cgccccgtga gccggcagcg cgaacggtct gcctcgccga tcgtgtggac
       61 gacggggtac tacccgtcgt tctggggcta ttacggctac ggctggagca gcgtcacgat
      121 cggcccggcg agggagaaca cgatcatcgt ggtcgaaacc ctcgtctacg acctgacccg
      181 tgaccagctt gtgtgggccg ccgtcagcga aaccaggaac cccgagacac tgcaggattt
      241 cgtcaaggac ctggtgaaag ccgccgtgag cgagatgaga aaggccaaac tcgtcggata
      301 gagcagacac gcgggggctg aagcccccac tctacgccct cggtccgggg ggacccccga
      361 cctgaacgaa gggccccacg agatttcgga ggctggcgcc agcaagagag cccagtcgcc
      421 caaggaacgg cagctctcgt ctcgattctc cgaccgtgtt tgtaaagttt gtagcacgcc
      481 ccgcgtggca tcaatcctgc cagcggggcg atgaggagtg gccatgagca caggaatgag
      541 ggtcgtggcg atcgccgtgg cgctcgttgc gccgtcggtc gtccacgcgc agtcggcggc
      601 gccgtccgcc cagaaccagc ctgaagagcg atccagcggg ctcccgcagg gtgtgagctg
      661 gacgttcaac ttcgacgccg gctggggcag cttcgggttc gccaattcgc tgttcgacaa
      721 cccgaaggac cccgtgcccg agaacctgag cgaccagtgg ttcgagggct acgtcaagcc
      781 agcgctttcg ggaagctacc ggtttgcctc gtcgagcgag atctacggca aggtcagcgt
      841 ggttggggag cgcacgtacg ggtcggcccc cgagctgtac ggtcctgacg tctcgtcgtt
      901 cggtcccgag gatctgtcga tcggctggcg atccggcaag tccatcggat cgagcgagaa
      961 cctcctcgac ttcaccgtcg gccgcacgca ataccgcctc gggcacggct tcctgttgta
     1021 cgacggcgcg tcggaagggg gaagccgcgg aggctactgg accaacgccc gcaaggcgtt
     1081 ccagttcgcc gcgatcggcc gcgtcaagcc tggcccccac ctgggcgagg tcttctatct
     1141 cgatcgggac gagctcgatg aaggcgacag cggaacgcgg ctggttggcg gcaactacga
     1201 gtactcgtac ggagagaata cgacgattgg cgtcacctac atgaagctgt tcgccttcga
     1261 cggcttcgcg gatccgaaac cgcaccgcga cggcctgaac gtcttcaacg tgcgcgcctt
     1321 taccgctccc gtgaccgcgc tgccggacct gtcgttcgag ttcgagtacg cggcggagcg
     1381 caacggcgac gcgctcgact ccaacgcatg gacgttgcag ggtgcgtacg agctgagcaa
     1441 ggtggcctgg acgccgaagc tgtcctaccg gtacgccctc ttcgagggcg acgatccaac
     1501 gacggcggcc aacgaggcgt tcgacccgct gctcctcggc ttctacgact ggggcacgtg
     1561 gtggcagggc gagatcgccg gcgagtactt cctctccaac tcgaacctgg tttctcatca
     1621 ggctcgggtg cacgtgcaac cctcggacgc catcggcggc ggcgtgatct acttcaactt
     1681 cctgctcgat cagccgcagg ctgttgcgcc gacggtgacc gagaaggacg tcgcgaacga
     1741 gatcgatttt tacacggact ggaaagtgac cgggaacttc acgctcagct tcgtcctcgc
     1801 gtttgccaat ccgggtacgg cggtgcagca ggcgacggga cgcacggagt ccttcagata
     1861 cgggatggtg tacattggct acagcttctg acgctcccgc ccggaccttc aagtttccga
     1921 ctgcctacac gatcctgttc gggctgatca tcgcggttgc ggcagcaacc tgggtggtgc
     1981 ccgcgggaca gtacgatcgc aagaagaacg agcagctcga caaggacgtg cccgttcccg
     2041 gcacgtacaa aaccgtcgaa ccgagcccgc agggcctgcg cgccgtcctc atcgcgccga
     2101 tcgcgggctt ctacaacccc gattcgaacc aggcggcggc gatcgacgtg gcgctcttcg
     2161 tgctcgtcat cggcggcttt ctcggcgtgg tcaccaagac cggcgcgatc gacgccggca
     2221 tcggcggggc gatggcgggg ctgaagggca acgagaagtg gatgatcccg atcctcatgg
     2281 cgctcttcgc ggcggggggc acgatctacg ggatggccga ggagtcgctg gcgttttacg
     2341 cgctcatcat cccggtgatg atcgcggccg gctacgatgc gctcaccggc gtcgccatca
     2401 tcatgctcgg cgccggcatc ggcacgctcg gctccacgat caaccccttc gcgacggtga
     2461 ttgcgtccaa tgccgccggc gtgccgttca cgacggggat gcctttgcgg ttcgcgatcc
     2521 tgggagcggg ctggctcgcc ggcgtcgtct acgtgatgcg ctacgccgag cgcgtgcgga
     2581 aggatccgtc caagtcgctc gtggccgaca tgaccaagag caaccgcgag cacttcctga
     2641 agtcgcgaag cgagtcgggc acgccggtgc tgacgggcgt ccgatcgctc atcctcgtca
     2701 tcttcggcct gagcttcgcg atcatgatct ggggcgtctc gtcggcgggg tggtggatgg
     2761 cccagatgtc cgcgctcttc ctggctgcgt cgatcctggt ggccctcatc gtgcgcatgg
     2821 gcgaggaaga cttcacgacg acgttcgtca acggcgcgcg cgacctgctc ggggtggcgc
     2881 tcgtcatcgg catcgcgcgc ggcatcgtcg tcatcatgga tgccggcaag atcaccgaca
     2941 ccatcctgtt ctggtccgag aaggcggtca acggcctggg cacggtcgcg ttcatcaacg
     3001 tcatgttcgt gctcgagctg gttctgtcct tcttcgtgcc ttcgtcgtcc ggtcttgccg
     3061 tgctgacgat gccgatcatg gcgccgcttg ccgacttctc gaacgtgggc cgtgacgtcg
     3121 tcgtcaccgc ctatcagtcg gccaacggcc tgctcaacct ggtcaatccc acgtttgcgg
     3181 tcgtgatggg cggcctggcg atcggacgcg tgccctacaa ccgctggctc cagttcacct
     3241 ggccgctggt cctcgttctg gcggtcatca tcgtggttgc cataagcctg gcggcgtaag
     3301 gcggttcgac gcgtccgcgt cggggtccct cgaggagaag agcgatgcaa cagatcggcg
     3361 ttcacagtga agttggtacg ttacgaacgg ttctcgtctg ccggccgggg ctggcccatc
     3421 agcggctgac gccgggcaac cgtgcggacc tgctgtttga cgatgtcctg tgggtgcacg
     3481 aagcccagaa ggatcactac gacttcgtcc tgaagatgcg cgagcgcggt gtcgaagtgc
     3541 tcgacctcca cgagctgctc gccgaaacgg tcgccgacaa agaggcgagg acatgggtgc
     3601 tcgatcggcg cgtgaccgcc aacgacgtgg gtccgggcat ggtgcccatc atgcgtccgt
     3661 ggctcgacga gatggccgct ccgaagctcg cggagcatct gattggcggc atcgccgtcc
     3721 tcgacctccc gaagtcggag caggcgacca tgctcgaaga ggcgtttggc gggaccgact
     3781 tcctcgttcc gccggttccg aacacgctct tccagcgcga tccgtcctgc tggatctaca
     3841 acggcgtgac ctgcaacccg atgttctggc cggcgcgcca gccggagacg ctcctccagc
     3901 gcgcggtcta caagtttcac ccgcgcttca agggcgccaa cttcacgatc tggtggggcg
     3961 actccgacga gtcgttcggc gccgccaccg tcgaaggcgg cgacgtgatg cccatcggca
     4021 agggcgtcgt cctcatcggc atgggcgagc gcacgacccg ccaggccgtg ttccaggtgg
     4081 cggcggcgct cttcaagcat cagggcgcca cgcgcgtgat cgggtgcctc atgccgaaga
     4141 gccgcgcggc catgcacctc gacacggtat tcacgttctg cgaccgcgac ctgtgtacgt
     4201 cgttccgcga agtcgccgat caggtccgct gctacagcgc gcggcccgac ggcaagggcg
     4261 gcgtcgacgt gcgccaggac aagggtcacc tcttcgacgt ggtcaaggag tcgatcggcc
     4321 tgaagacgct ccgcgtcgtc gagactggcg gcaacgcctg ggaggcgcag cgcgagcaat
     4381 gggacgacgg caacaacgtg gtcgcgctca gccccggcgt cgtcgtcggg tacgaccgca
     4441 atactcacac gaacacactg ctgcgcaagg ccggcatcga agtgatcacc gtgcgcggat
     4501 ccgagcttgg ccgcggccgt ggaggcgggc actgcatgac ctgcccgatc tcgcgcgatc
     4561 cggcgtattg atcgcgattt caacgaagtc gaccaggagt tccgaggaca caccatgagt
     4621 ttcaacctgc gcaatcgctc actgctgacg gtacaggact acacgacccg tgagttccgc
     4681 tacctcctcg acctcgcccg ggacctcaag cgggcgaagt acgcgaagac ggaacagaag
     4741 catctcgagg gcaaggagat ctgcctgatt ttcgagaaga cctccacccg cacccgctgc
     4801 gccttcgagg tggcttgtta cgaccagggc gcgcacgtga cgtacctgga ccccgccggg
     4861 tcgcagatcg gccacaagga gtcgttcaag gacacggcgc gcgtgctggg gcggatgtac
     4921 gacgcgatcg agtatcgcgg cgccagtcag accggcgtcg aggagctcgc caagtacgcc
     4981 ggcgtcccgg tgttcaacgg cctcacggcc gaataccacc cgacgcaaat gctcgccgac
     5041 gtgttgacga tgcgcgagca ctccgacaag ccgatccacc agatcaagta cgcattcatg
     5101 ggcgacacgc gcaacaacat gggtcactcg ctgatgatcg tgggctgcct gaccggcatg
     5161 gacgtgcgca tctgcgggcc caggaagctg tggcccgctg acgagtacgt cgagatcgcg
     5221 aaaaagctgc aggaacagtc cggggccacg ctcaccatca ccgacgaccc gaagaaggcg
     5281 gtcgccggcg tggacttcat tcacaccgac gtctgggtgt ccatgggcga gccgaaggag
     5341 gtgtggaagg agcggatcga cctgctgctg aagtaccagg tcaacatgga gctcatgaag
     5401 gcgagcggca acccgcaggt caagttcatg cactgcctgc cggcctttca cgacaccgag
     5461 acgaccctcg gcaagcagat cgcgcaggac tacgggctga agaacggcct cgaggtgacc
     5521 aacgacgtgt tcgagtccga gtacaacatc gccttcgaac aggccgagaa ccgcatgcac
     5581 acgatcaagg cgatcctcgt cgccacgatg ggggactgag ccatgcttgt cgtcactgcc
     5641 ctgggcggca acgcgctcct gcggcgcggc gagccgatga ccgccgagaa ccagcggcgg
     5701 aacgtccgca tcgccgcgga ggcgctggcg ccgattgccg ccgagcctca gctcgtcgtc
     5761 ggccacggca acggcccgca ggtcgggctc ctggcccttc agggggcggc ctacaccaag
     5821 gtcgaaacgt atccgctcga cgtgctgggc gccgagaccg aggggatgat cggctacatg
     5881 atcgagcagg aactcggaaa cctgctgccg ttcgagcgtc cgttcgccac gctgctgacg
     5941 atgatcgagg tcgatcccgg cgatccggcg ttcaaggatc ccaccaagtt catcggcccc
     6001 gtgtacggca aggaagaagc cgaccggctc gcggccgaga aatcgtgggt cttcaagaag
     6061 gacggcgaca agtggcgccg cgtcgtgccg tcgcccgagc cgaagcgcat cttcgagctg
     6121 cggccgatca agtggctgct cgagcggaac acgatcgtca tcgccgcggg cggcggaggg
     6181 attccgacga tgtacgagcc gggcaaacag cggaagctgg cgggtgtcga gtgcgtgatc
     6241 gacaaggact tcgcgtcgga gctcctggcc cgcgatctcg aggccgattt gttcatcatg
     6301 ctgacggatg ccccggccgt attcgtggac ttcggcaagc cgacgcagaa ggccatacgg
     6361 cgggcgtccc cggccgcgct gtccgcgatg tcgttcgcgg ccggctcgat gggcccgaag
     6421 gtggacgcgg catgccgttt cgcaaaggcc acaggcaaga aggcggctat cggtgctctc
     6481 gctgacctca gcaagatcct ggcaggtgaa gccgggacga ccgtgtccac gaaggagacc
     6541 ggcattgtct atgcctgatc ccccgtccgc agatcgtaga gcgggggctt cagcccccgc
     6601 gaagagcctc ggcctggccg cgtgcacggc gatcgtcgtc ggcaacatgg tgggctcggg
     6661 gttctacctc tcgccgtcgg cgctggcgcc atacggtttg cttgcgatcc ttgcctggat
     6721 cgtgatgggc gccggggcgg tctgtctggg cctgacgttc gcccggctcg ccagcctcgc
     6781 ccccgcgacc ggtgggccgt atgcctactc gcggatgggg ttcggcgact ttgcgggctt
     6841 cctcgtggcg tggggctact ggatctccat ctgggcatcg ctgcccgcca tcgccgtggc
     6901 gttcactgga tcgctcttca agctcgtccc gaacctgggg ggccggcccg tcgccgtcgc
     6961 ggtcacgctc gcagccatgt ggttcgtcgt attcacgaac cttcggggcg tcaaggaagc
     7021 gggcgtgttc gcccaggtca caacgtggtc caagctggtg ccctttgccg cgatttcgat
     7081 catcgggctg ctctacgtga gtcccgagaa cctgagtcag ttcaatccca gcggtcagac
     7141 gcttctggcg tcgagcgccg cgctcgcgcc gctgaccatg tttgcgtttc tcgggctcga
     7201 gtccgccacc gtcccggccg gcgacgtcat tcagcccgag cggacgattc cgcgatcgac
     7261 gattctcggc gtgttgatcg ccgcgttgct gtatgtcctc ggcacgacgg ccgtcatggg
     7321 cgtggtgcct cgcgagcagc tcgtgaagtc ggtggcgccc ttcgccgacg cggcgcagat
     7381 catgtggggc acctgggccg ccggcgtggt gggcgtcgcg gtgatgatct cttcactcgg
     7441 ggcactgaac ggctggacgc tcctgatggg ccaggtgccg atggcggcgg cgcaggaccg
     7501 gctgtttccg ccgctgttcg ggaaagtgtc tcctcgcggc gtgccggccg tgggcatcgt
     7561 ctgttccgcc gtgctggcca cggtcctcgt cttgatccag gccatggccg cgtcgggctt
     7621 cgccgccttt tacagcctcg tcgtcaacct ggcgacgatg acggccgtcg tgccctacgc
     7681 gttctgctcg ctggcggtgg ggctgatcgc ggtgaaggga agcgggggaa ggccgaagcg
     7741 cggcatcggt ccgatcgaag tcatcgcgtt catcttctcg atattcacgc tctacgggtg
     7801 cggtcccgaa gcggtcctgt acggcttcgt gctcctcatg ctcggcatcc ccgtcttcgt
     7861 ctggcaaagg cgcgcgtcgg ccgcctgatc ctcacattac tcgagcaaga caccaggtca
     7921 cgctaaagcc ggacactacg tacgcccgcg gcggcgcgca acgcgggtga cgagaccgcg
     7981 aaccgcaaag tacggcacga gcgccagaac cgtcgcggtc agcagaagct cgagcgggta
     8041 caccatcgaa tgcacgatga cctggtagat ggcgtcgagc ccgatcgcca cgaagaacag
     8101 gttccgtacg tcattccacc cgtgccggag caagtggggt ctgcggtcgc gattcgagag
     8161 aagcgtccac aggaagggcg gctgaccctc acgcgcgtcc ctgagtccgg agcggaccgc
     8221 caggatgatc gccatcgcag gctgcatgac cagccgaaaa ttcatcggtc cgtcgggacg
     8281 gccgacgacg ttctcccacc cgcgcacgag cgcctcctgc acgaaggaga gcatcgcggc
     8341 taatagctcc agccaagcga tatgaccacg ccgacgtcgt tccgatcggc gtcggggttg
     8401 ggcgggtcgc tgtcgaaggt gtcgaagaga ttgagcgaga cgtagaagtc cttcagcagc
     8461 tcgcgcctga cgctggtgtc gaactgcagg cgctggcggc cgaagttgct gatgctgggg
     8521 tagtactgaa agctgacgtc gaggttcgtc ttcggacggt cgtacgtgta gtacgaggcc
     8581 cggaaggcga gcagaccctc gaggttctga gtggcctcga catcggtccc ctgctcgtgg
     8641 ttgacgacca tcccgccgcc ggccgccatc tccccgtgtg agccgttgaa caaggtcggc
     8701 ccgacaacgg cgccgacctg ggacctcagc tcgaggccga gactctcgtt cgtctccagc
     8761 cggcccatgg ccacgatgaa ccagcgctgc caggggttga tgagatacga cgcctcgagc
     8821 gctccacggt cgtcgctcga gccgtcgctg gtcgacgtct gggtggccga cgccgtcagg
     8881 cgcacctgcg acgcgggctt gcgatagacc gagtcccagt tcaggttgaa ctgttccact
     8941 ccgctcgaac gggtatagct gaacccgacg tcgacggagc cgtcgagttt ggtccagaag
     9001 cttcttccga tggcggtgat gtatgtcacc tcgctcatct ggagggtcac gcttccatcc
     9061 ggactcgcca ccccaatcga acccgggccg gcctggccga ggctgccgag gaaacggcgg
     9121 ctgtcgaccg taatcacttc aacgagctgc gtcgtcacga ctgtcgcgag cttgtcccac
     9181 tcgaggtaca gcgtgcccgc atcgtcggtc tcgaactcga gccggcctcg ctcgagccgc
     9241 acgacctcgc cggtgattcg atcgccgttg cggagcgtca cgacgtccgt acgtccctga
     9301 ccgtgcagcg tcgcgcccag ccacatcacg agacccacgg caatcgtcgc cctccctcga
     9361 gggcggaagc gcgcgttccg tgcgagggcc acgacggcgg taggcgctcg ctcagaactt
     9421 gaagacgacg gctccgctgg cccgcccgaa gtcgagcttc gtgtcggttt cgattccgaa
     9481 cacgttcaga agtgcgaccg agtgaaagta gcggacatcg ccgcgtatgc cgacatggga
     9541 gccgaagaag atcatcaggc cgccgccgac gtcccacccg aagtcgttct gatcggtatc
     9601 ggtgaccagt ggaggagtcg agtccacctt cgtcttgatc aggccggccc cgatcagccc
     9661 gtagggttga acgggcccga acttgggcgc gagcaggaaa ttgcccatga aggtcgtgac
     9721 gctggacttg ctcgtcggcg tttcgccgaa gaagtcgtcg atgtaggccc attcgccttc
     9781 gacgccgaag atgctcccga ggaagccgaa cccgaccccc cagttcaggt gcttgtcctc
     9841 gcagttggtg atcgccggac aaccggaatc gccgctgaag ttgtagccga tgagcgggct
     9901 gatgaagccc tgggccgacg cctcctcggt tgaccacatc gtgcacgcac ccgcgagaag
     9961 ggcaacgccg gccagccgtt tcatctgtga acgtacagtc atccagtcac ctcgaatcga
    10021 tgtaccgaca agcagtgtag aacaatgtcg cccggcgcgg tggcaaacac gcccggcaga
    10081 ggttgctgat ggcggggcta ggcggcggtc gaggccgggg gcacccgtcc cccgtgcgag
    10141 tctccgcgcc cgcgcggccg cggtcccgct cccggtgcac cgtctggatt ggcgtcgtcc
    10201 ggccactcga atggtgccga cgaaaagaac acctcggtct gcgtctcgat cgccctcgag
    10261 cgggtagacg gaccccggtc cccgagccgc cggtgcgacg ccagcagctc ctcggcggcc
    10321 tcgcgcgaca ggggaggcga gaagtagtag ccctgggcgt ggcggcagcc gaggcgctcg
    10381 agctcccgtg cctgaacatc gctctcgact ccctcggcga cgacgcccgt gtcgagcgtc
    10441 ctggccagcg cgaggatgct ctcgacgatg gccggccgat cgggcagcag caggcttctg
    10501 acgaacgagc gatcgatctt gagcgcgtcc acgggaagct tgtgcaggtg gctcaacgac
    10561 gaatagcccg tcccgaaatc gtcgagatag atcttcacgc cgaagtcctg cagctgacga
    10621 agcgcgtccg ctgccacgaa ggggctgccc atcaacgtcg tctccgtgat ctcgaggcgg
    10681 aggtctgccg gcgtcatcct cgcctcgtga accgcctgct cgacgatgtg gaggaagttc
    10741 tgctgcatga gctgacgggt agacacgttg accgtgatgc actccagctt gttctgcgga
    10801 aaccttcgct gccagtccgc gaaggtacgg caggcttccc tcagcaccca cgtgcccaga
    10861 ggctcgatga gccccagctc ctcggcgacc gggatgaact tgtccggggc gaccgtcacg
    10921 ccattgcgcg tccagcgcac cagggcttcg aacccgacgc acatcccgga cgtcagggac
    10981 acgatcggct gatagtggac ctcgaagtcg gtgtcggtca cggcgtgacg caggtcgttc
    11041 tcgaggccga gccgatcgag ggcgcgcgag cgcatgtcgg catcgaacac ctcgtgccgc
    11101 gccttgccgc tcgccttggc ctggtacatg gcgctgtccg cgtcgcggat gatctcctca
    11161 gggttgttgt actgggacgc gctgaaggtg atgccgatgc tcgccgacgt gaacacttcg
    11221 cggccgccga tcgagaacgg cacgttcagc gcgtcctgaa tcctgaacgc gatggcgttg
    11281 gcctgcccct cgtcgtcgag tgagttcagc aggagcgcga actcgtcgcc gccgagcctg
    11341 gcaatggtgt ctccctcccg
//
LOCUS       DBNGSH010000358         2385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_539620, whole genome shotgun sequence.
ACCESSION   DBNGSH010000358 DBNGSH010000000
VERSION     DBNGSH010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_539620"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1448
                     /locus_tag="AC16CY_08450"
     CDS             84..1448
                     /locus_tag="AC16CY_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3353856.1"
                     /translation="MDWANGRGQRVSVAARLNRLPPIRTHRRATIIAGIGTFFDLFDI
                     FLAGVLGTVLTREFQLDRLALPAALGSAFVGMFIGATFLGRFADHFGRRTAFLLNLSI
                     YSAFTLAGAFSTSATMLIATRFLAGIGIGAELPLVDAYLGELLPARLRGRYTAWAYTL
                     GFVGIPAAGLLGRILVPLEPLGIDGWRWMFVAGSLGAVIVWFVRTSLPESPRWLESVG
                     RTAEAEAIVARMEEEASAAGPLPPPSSADTATVARARLSVLFGTRYRSRTIMLGVFHI
                     FQAVGYYGFGTLVPLVLASKGYSVVTSLTFTTLTFIGYPVGSALSLPIVERIERKWLI
                     AGSALLMSVFGIAMGYASSPAAIVVLGFLYTAVSNVFSNGLHIFQVEIFPTYIRATAA
                     GLCYGLSRLSSAAMPFVLLPVLDDWGAGPMFTVVAAALWIVILDVALFAPRTTGRSLE
                     EAST"
     gene            complement(1576..>2385)
                     /locus_tag="AC16CY_08455"
     CDS             complement(1576..>2385)
                     /locus_tag="AC16CY_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3353857.1"
                     /translation="RLGDNLYSCSVIALDPDTGTLKWHYQFSPHNEFDWDSAQIPVLA
                     DMDWQGRPRKVMLWANRNGMYYVLDRATGQFLKGAPFVKVNWADGFDDKGRPIPVPGI
                     VPTKEGTLVYPGNQGGTNWYSPSYSPRTGLFYIPTWENTSTTYIKADTPPVFKEGQSF
                     TGLFPRGGSTADDVHSTIQAIDPRTGQKRWSHRLAAPMTEAGVLTTASDLLFSGGRDG
                     AFYALDARTGALLWQTNLGPSVAAGPMTYAVGGRQFVAVMAGGSMYAFGLR"
BASE COUNT          333 a          793 c          790 g          469 t
ORIGIN      
        1 cacgtcgagg acccggccga ggcagccgaa tgacgctata gaattggcgc ctcgctcggc
       61 aggagcctgt cgatggaccg cgcatggatt gggccaatgg acgggggcaa cgcgtatcgg
      121 ttgcggcgcg gctgaaccgg ctgccgccga tccggacgca ccggcgcgcg acgatcatcg
      181 ccggcatcgg gacgttcttc gatctcttcg acatcttcct ggcgggcgtg ctcggcacgg
      241 tcctgacgcg ggagtttcag ctggatcggc tggcgcttcc cgctgcgctc ggctcggcgt
      301 ttgtcggcat gttcatcggc gcgacgttcc tcgggcgatt cgccgatcac ttcggacggc
      361 ggacggcgtt tctcctgaat ctctccatct actccgcgtt cacgctggcg ggcgcgttca
      421 gcacgagcgc gacaatgctc atcgcgacac ggttcctggc cggcatcggc atcggcgcgg
      481 agctgccgct cgttgacgcg tacctgggcg agctgctgcc ggcgcgcctg cggggacggt
      541 acaccgcgtg ggcctatacg ctcggcttcg tcggcatccc ggccgcgggc ctgctcgggc
      601 gaatcctggt gccgctcgag ccgttgggca tcgacgggtg gcgctggatg ttcgtcgccg
      661 gatcgctcgg cgccgtcatc gtctggttcg tgcggacgtc gctgcccgag tcgccgcgtt
      721 ggctcgaatc ggtcggccgg accgccgagg ccgaggccat cgtcgcgcgc atggaggagg
      781 aagcgtcagc ggccgggccg cttccgcctc caagcagcgc cgacaccgcc acggtcgcgc
      841 gcgcgcggct gtcggtgctg ttcggtacgc ggtaccggtc ccgcacgatc atgctcggtg
      901 tcttccacat cttccaggcc gtcggctact acgggttcgg cacgctggtg ccgctcgtgc
      961 tggcctcgaa ggggtactcg gtcgtgacgt cgctcacctt cacgacgctg accttcatcg
     1021 gctacccggt cggctcggcg ctctctctgc cgatcgtcga gcgaatcgag cggaagtggc
     1081 tgattgcggg ctcagcgttg ctcatgagcg tcttcgggat cgccatgggc tacgcctcat
     1141 cgcctgccgc gatcgtcgtg ctcgggtttc tgtataccgc ggtcagcaat gtcttttcga
     1201 atggcctgca catctttcag gtcgagatct tcccgaccta catccgggcg acggcggcgg
     1261 gcctctgcta cggcctgagc cggctgtcga gcgccgcgat gccgttcgtg ctgctgccgg
     1321 tcctcgacga ctggggcgcg gggccgatgt tcaccgtcgt tgcggcggcg ctgtggatcg
     1381 tcatcctcga cgtcgcgctg ttcgcgccgc ggacgaccgg gagatcgctc gaagaagcga
     1441 gcacctgacc ctctacgctg agatcacgga cgttaatcac agagacacag aggacacaga
     1501 gtttggtctt tcagatccct ctgtgttctc tgtgcctctg tggttggcgt ccgtacaatt
     1561 tgcgctaacc ggcgcttatc gcaggccgaa ggcgtacatc gagccgccgg ccatgacggc
     1621 gacgaactgc ctgccgccga cggcatacgt catcggaccg gcagccactg aaggtccgag
     1681 gttcgtctgc cacaacagcg cgccggtgcg cgcatcgagc gcgtagaacg cgccgtcgcg
     1741 cccgccgctg aagaggaggt cggacgcggt cgtcagcacg cccgcctcgg tcatcggcgc
     1801 tgccagcctg tggctccatc tcttctgccc ggtcctcgga tcgatcgcct ggatggtgct
     1861 gtggacgtcg tcggccgtcg aaccgcctcg cggaaacagt ccggtgaacg actgtccttc
     1921 cttgaacacc ggcggcgtgt cggccttgat gtacgtggta gacgtgttct cccacgtcgg
     1981 gatgtagaac agcccggtcc gcgggctgta cgacgggctg taccaattgg tgccgccctg
     2041 gttgcccgga tacacgagcg ttccttcttt tgtcggcacg atccccggaa ccgggatcgg
     2101 tcttcccttg tcgtcgaacc cgtcggccca gttcaccttg acgaacggcg cgcccttcag
     2161 gaactgcccc gtcgcgcgat cgagcacgta gtacatgccg ttgcgattgg cccacagcat
     2221 caccttgcgc ggccgcccct gccagtccat gtcggcaagc accggtatct gggcggagtc
     2281 ccagtcgaac tcgttgtgcg gcgagaactg gtagtgccac ttgagcgtgc ccgtgtccgg
     2341 atcgagcgcg atcacagagc agctgtagag gttgtcaccc agtcg
//
LOCUS       DBNGSH010000359         4662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1388835, whole genome shotgun sequence.
ACCESSION   DBNGSH010000359 DBNGSH010000000
VERSION     DBNGSH010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1388835"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /gene="manD"
                     /locus_tag="AC16CY_08460"
     CDS             <1..1129
                     /gene="manD"
                     /locus_tag="AC16CY_08460"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HIR3353858.1"
                     /translation="GIYGLGDATLNGRELAVASYLRDHVLPLLEGRDARRIEDVWQVL
                     YKGAYWRRGPVTMCAIAAVDTALWDIKGKSLDAPVYQLLGGASRDGVRVYGHASGQTV
                     EETVEAVGRFLSRGYTAVRAQTSVPGLAKTYGVGRGDAYEPAERGALVETAWDSARYL
                     RSVPPLFERLRREFGPGPHLLHDVHHRLTPIEAAGLGKSLEPYHLFWLEDPTPAENPN
                     GFRLIRRHTTTPLAVGEVFNTIHDCRELVEEQLIDYIRTAVVHAGGITHLRKIAALAE
                     LHQVRTGCHGATDLSPVCMAAALHFDLSVHNFGIQEYMPHAADTDRVFPHQYRLDSGM
                     MHPGDAAGLGVDIDEDLAATFPYERAYLPVARLDDGTMHDW"
     gene            complement(1168..1563)
                     /gene="vapC"
                     /locus_tag="AC16CY_08465"
     CDS             complement(1168..1563)
                     /gene="vapC"
                     /locus_tag="AC16CY_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3353859.1"
                     /translation="MVIVDSTVWVDYFNGLHNPETDWLEVQLDQQRLGLTTLILCEVL
                     QGVRDERKAVAVETDLMKLEVFEMPSVVLAVQAGKHYRTLRSRGRTVRKTIDVLIATF
                     CIEEQHSLLHRDRDFDPFEELLGLSVVHP"
     gene            complement(1570..1773)
                     /locus_tag="AC16CY_08470"
     CDS             complement(1570..1773)
                     /locus_tag="AC16CY_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HIR3353860.1"
                     /translation="MRTNIDIDDRLMRQAMRSSGARTKRAVVEEGLRLLIQTRGQASL
                     RRLRGKVAWEGDLHASRLERTSE"
     gene            complement(2416..4242)
                     /locus_tag="AC16CY_08475"
     CDS             complement(2416..4242)
                     /locus_tag="AC16CY_08475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3353861.1"
                     /translation="MAKHNVVVLNSGAGGNGRHVARLLAFAIALAVPATALHHESFQP
                     LERRVELQGRTADWSFELPSAESLYLEIGVIVRTSGTGSADVAVDVNGTELSRLRPDT
                     LYAIKRSIVAVPAALTRAGTNRLGFRVQGPADATFDVRVRVHNYYGISPKFPRATVVS
                     DEAARRFFAEQGWAGLAPRFGLFVLAGLGAAVVARRLSRMTAARERLLMAAATALPLA
                     AFVGAVATPYHFWVPASTIAVLVLTPVLVTGAAMWTGGHWGLVSPYVWSAAITLVLLE
                     GGLRVFNAVYPTPILSVGADDRFRGRPGARFFDSHLNSRGFNDVEHAPAKPPGVRRVV
                     ALGDSVTVGVVPYSANYLTLLENDLGREGRVDVINMGVSATGPRDYRRMLAGEGLAFS
                     PDLVLVSFFIGNDFEADVTRAYERSSAATFFYFLWRLATAGSASVAGFDPGATEYRDD
                     APTYGEDRFLEIEVDRARIHQIGNPLLDTWMPAVLDSLREIQRTSRRAGADLAVVIVP
                     DELQVDAALQAQVVRAYRAGPGDFDFARPNRLLAEALAADGIAYLDLLPEFLKHAPTR
                     LYKPRDTHWNLAGNKLAADTIAPFLRERLGSRRSPPPAANGR"
     gene            4310..>4662
                     /locus_tag="AC16CY_08480"
     CDS             4310..>4662
                     /locus_tag="AC16CY_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353862.1"
                     /translation="MVLTKSELIGSLKNEVRILLHLASKIDRGMLDYRPTPKQRSTLE
                     LLQYLSIMGPSLIEAARRGAFDPAAWTVAEQAASARDFDQTIAAITAQTDSYARLLTD
                     LSDADFRAEITMFGDT"
BASE COUNT          826 a         1562 c         1542 g          732 t
ORIGIN      
        1 cgggatctac ggactcggcg acgccacgct gaacggacgc gagctcgccg tggcgagcta
       61 cctgcgcgat cacgtgctgc cgctgctcga gggacgtgac gcgcgccgca tcgaggacgt
      121 ctggcaggtc ctctacaagg gcgcctactg gagacgcggg cccgtcacca tgtgcgcgat
      181 cgccgcagtg gacacggcgc tgtgggacat caagggcaaa tcactggacg cgccggtgta
      241 ccagctgctc ggcggcgctt cgcgtgacgg cgtgcgcgtc tacggtcatg ccagcggcca
      301 gaccgtggag gaaaccgtcg aagcggtcgg ccgctttctc tcgcggggct acacggccgt
      361 gcgcgcgcag acgtcggtac cgggactggc gaagacctac ggcgtcgggc gaggcgacgc
      421 ctacgagccc gccgaacggg gtgcgctcgt cgagaccgcg tgggacagcg cgcgctatct
      481 gcgcagcgtg ccgccgctct tcgagcgcct ccggcgcgag ttcgggccgg gcccgcatct
      541 gctgcacgac gtgcaccatc ggctcacgcc gatcgaagcg gccgggcttg gcaagtcgct
      601 cgagccatat cacctgttct ggctcgaaga ccccacaccg gcggaaaacc cgaatggatt
      661 ccggctgatc cggcgccaca cgacgacgcc cctcgcggtg ggcgaggtgt tcaacaccat
      721 tcacgactgc cgcgagctcg tcgaggagca gctgattgac tacatccgga ccgccgtcgt
      781 ccacgcggga gggatcacgc atctgcgaaa gatcgcggcg ctcgccgagc tgcatcaggt
      841 tcgaaccggc tgccacgggg ccaccgatct cagcccggtc tgcatggcgg cggcgctgca
      901 cttcgacctc agcgtgcaca acttcgggat ccaggagtac atgccgcacg ccgccgatac
      961 ggaccgtgtg ttcccgcatc agtaccggct cgacagcggg atgatgcacc cgggcgacgc
     1021 agccggtctc ggcgtggata tcgacgaaga tctcgcggcg acgttcccct acgaacgcgc
     1081 gtacttgccg gtcgcccgcc tagacgacgg cacgatgcac gactggtaga acggttgtcc
     1141 ggggccgcag gtgtccgtga gatcagacta cgggtggacg acagacaggc ccaggagctc
     1201 ctcgaaggga tcgaaatcgc ggtcgcgatg gagaagtgag tgctgctcct cgatgcagaa
     1261 cgtcgcgatc aagacatcga tcgtctttcg cactgtccgg ccgcggctgc gcagggtccg
     1321 atagtgcttg ccggcctgga ccgccaagac cacgctgggc atttcgaaga cttcgagttt
     1381 catcaaatca gtctctacgg ccacggcctt ccgctcgtcg cgaacgccct gcaacacttc
     1441 gcacaggatg agcgtcgtca gcccgaggcg ttgctggtcc agttgaacct cgagccagtc
     1501 cgtctcggga ttgtgcaggc cattgaagta gtcgacccac acggtactgt cgacgatcac
     1561 cacgggcggt tactccgagg tgcgttcgag ccgtgacgcg tggaggtcgc cctcccaggc
     1621 taccttccct ctcagacgac gaagactggc ctggccacgg gtctgaatca acaaccgtaa
     1681 cccctcttcg accacggcgc gcttggtcct tgccccgctg ctccgcatcg cctgccgcat
     1741 cagccgatcg tcgatgtcga tgttggtccg catgatccgt atgtgtaagg atcgcacaat
     1801 cgtgtgtatc catcaacgca cgcgcgcgcg cgtcctggaa cgccctgtgt gcaattttgc
     1861 acagacgcct cacgcacggc tccgttaggg tacggctcgt ttttcgatca tcccgccatg
     1921 cattccgtaa gcccgcgcct ccagagagtc atgcacagga ccacagcgtg ttgctcgcga
     1981 ctgtggcgga ggagtccgtt atgtcaacgc ctgatgtgct tgtctcgcgc gtgaaggggg
     2041 agtaccgcga gatgcccgga ctcaagctca cgttcgcgca ggcgtgtcgc ctctggtgcc
     2101 tggagcgcga cgcctgcgag gctgtgcttc gggccctgct ggaggaacga ttccttgggc
     2161 ggacggcaga cggcgcgttc gtggcccttc catcaccggg cgacgcacgc gccactcctc
     2221 tcaaggccca gtctcccgcg ccaccgcagg ctcacccgga cgacgccgtc cgtcgcgatc
     2281 cggtttcggg gaaggtcgtg ggcgaactcg atcagcgacg acctcaaatg tcgcattctg
     2341 agccgcgatc aagaccgtcg cgactacgac tccgaagagg gtgaaggcgg cctgcggatt
     2401 gccgtgccaa cgcggtcagc gaccgttggc ggccggcggc ggacttcgcc ggctgccaag
     2461 gcgctcgcgg agaaagggcg caatcgtgtc ggccgccagt ttgttgccgg cgagattcca
     2521 gtgcgtgtcg cgcggcttgt acagccgggt gggcgcgtgc ttcaggaatt ccggcaacag
     2581 atccaggtac gcgatgccgt cggcggcgag cgcctcggcg agcagccggt tcggacgggc
     2641 gaagtcgaag tcgccgggtc cggcccgata ggcccggacc acctgggcct gaagggccgc
     2701 gtcaacctgc agctcgtcgg ggacgatgac gacggccaga tccgcgcccg ccctccgcga
     2761 cgtccgctgt atctcccgca gggagtcgag caccgcgggc atccacgtgt cgagcagcgg
     2821 gttgccgatc tggtgaatcc gtgcccgatc gacttcgatc tcgagaaagc gatcctcccc
     2881 gtaggtgggc gcgtcgtccc ggtactccgt cgcgcccgga tcgaagccgg cgacggaggc
     2941 ggacccggca gtggcgagac gccacaggaa atagaaaaac gtggccgcgg aagaccgttc
     3001 gtaggcgcgc gtgacatcgg cctcgaagtc gttcccgatg aagaaagaca cgagcacgag
     3061 gtccggcgag aaggcgagcc cctcgccggc gagcatccgt cgatagtcgc gcggccccgt
     3121 ggccgacacg cccatgttga tcacgtccac ccggccctcg cggccgagat cgttctcgag
     3181 aagcgtcagg tagttcgccg agtacggcac aacgccaacg gtcaccgagt cgccgagggc
     3241 gacgacgcga cgcacacccg gcggctttgc gggcgcgtgc tcgacatcgt tgaagccgcg
     3301 cgagttgagg tgtgaatcga agaagcgtgc gccgggccgt ccgcggaaac ggtcgtcggc
     3361 gccgaccgac aggatcggcg tcggatagac ggcgttgaaa accctcaggc cgccctcgag
     3421 caggacgagc gtgatcgccg cgctccagac atagggcgag accagccccc agtgtccccc
     3481 ggtccacatc gccgcgccgg tcacgaggac aggcgtcagg acaaggacgg caatcgtcga
     3541 ggcgggcacc cagaagtggt acggcgtcgc gaccgcaccg acaaacgcgg ccagcggaag
     3601 ggccgtcgcc gcggccatca gaagccgctc cctcgcggcc gtcatccgcg acagccggcg
     3661 cgccacgacg gccgcaccaa ggcccgccag gacgaagagg ccgaaacggg gggcgagtcc
     3721 ggcccatccc tgctcggcga agaagcgccg ggccgcctca tccgacacga ccgtcgcccg
     3781 cgggaacttc gggctgatgc cgtagtagtt gtgcacgcgg acgcgcacgt cgaaggtcgc
     3841 gtcggcgggt ccctgcacgc gaaacccgag ccggttcgtg ccggcgcgcg tgagcgcggc
     3901 aggcacggcg acgatcgagc gctttatcgc atacagcgtg tccggacgca gacgagacag
     3961 ctcagttccg ttgacgtcca ccgccacgtc cgcgctgccg gtgccggagg tgcgaacgat
     4021 gaccccgatc tcgaggtaca gcgactccgc ggaaggaagc tcgaaggacc agtcggcagt
     4081 cctgccctgc agctcgaccc ttcgttcgag cggctggaag gactcgtggt gcagggcggt
     4141 cgccgggacc gccagcgcaa tcgcgaacgc caggagccgc gcgacatggc gcccgtttcc
     4201 cccggcgcca ctgttgagca cgacgacatt atgcttcgcc acggtgagct gccgctcgac
     4261 acatccttgc agttggccgt gctatccttc cggcttttgc gagggaaaca tggtcctgac
     4321 aaagtccgag ctcattggat cgttgaagaa cgaagtacgc atcctgcttc atctggcgag
     4381 caagatcgat cgcggcatgc tcgactaccg cccgacgcct aaacagcgca gcacgctcga
     4441 actgctgcag tacctgtcga tcatgggacc gtccctcatc gaggccgcca ggcgcggagc
     4501 gttcgaccct gccgcctgga ccgtggccga acaagccgcg tcggcacgtg atttcgacca
     4561 gacgattgcc gcgattacgg cgcagaccga tagctacgcg agactgttga ccgatctgtc
     4621 tgacgccgat ttccgcgccg agatcacgat gttcggcgac ac
//
LOCUS       DBNGSH010000360         4509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2917496, whole genome shotgun sequence.
ACCESSION   DBNGSH010000360 DBNGSH010000000
VERSION     DBNGSH010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2917496"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..654
                     /locus_tag="AC16CY_08485"
     CDS             199..654
                     /locus_tag="AC16CY_08485"
                     /inference="COORDINATES: protein motif:HMM:NF037832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR3353863.1"
                     /translation="MASELGGHAERVSGHPRVYVDANVPAGLVSYMRQTLHWDVLFVL
                     EHDDLRRARDGEHFRRAKHLRRTLITLDRDYLDDRRFPPSESGGVLVLTAPAERGLAA
                     LLKRLDREMLRRTAPSDRGDVEVLAPDPLPLEGRKLHVHVDWSSEAVAP"
     gene            651..1829
                     /gene="nagA"
                     /locus_tag="AC16CY_08490"
     CDS             651..1829
                     /gene="nagA"
                     /locus_tag="AC16CY_08490"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR3353864.1"
                     /translation="MIVVSGGDLVLPGRIVTEGALVIDGERIVAIEPHRVDPADAKVV
                     DATDCYVVPGFIDVHVHGLHGHDTLDDGDAVARIAAHMPRYGVTAFCPTTVACPPAAL
                     RRVLQQVRQLRSTPSAGSARVLPAHLESNFINPEYRGAQPAACLRTPAGTHKDGEFAG
                     REVLDVIAASRPDVGIVTLAPELPGGIDLVRALVAAGHRVSLGHSGADLDVAFAAIDA
                     GARHATHLFNRMTPMTHRAPGLPGAVLARDEVAVELICDGVHVHPAMCQLAMAAKGPQ
                     GVMAITDATSGAGLPQGSASRLGGQTIHVRDGAAFLDEGTLAGSTLTMDRAFRHIVSR
                     FGGSIVDAAIMCSTTPARGLGLTGFGVLADGAVADLVVLDRGFHVVRTFIAGVEVWRT
                     "
     gene            complement(1851..>4509)
                     /locus_tag="AC16CY_08495"
     CDS             complement(1851..>4509)
                     /locus_tag="AC16CY_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3353865.1"
                     /translation="GGMGEVYRARDSRLQRDVAVKVLPDLFASDPERLARLEREAQVL
                     ASLTHPNIGHIYGLEDLPADAGPRGRALILELIEGPTLADRIVEGAIPVSDAIALARQ
                     IADALDTAHLHGIVHRDLKPANIKVREDGTVKVLDFGLAKFTENPSAGLQSPTSIPSM
                     SPTMSAAFTGAGVILGTAAYMAPEQARGRAVDKRVDVWAFGCVLFEMLTGKRTFDGTD
                     ATEMIAAVVRGEPEWSLLPAAATPRLRTLLERCLAKDPNRRLRDIGDVRYELERALEQ
                     PAAEKAAPAARPPIWRSALTYAAALIAGGGVASYAVWTLRPEPVRPLARFSVALPEGQ
                     RFTNTGRHLVAVSPDGTRIAYSANNQVFMRAIEQLEPVPVRGTAGEGNGSGRNPFFSP
                     DGQWLGFWQDGEIKKVPVSGGAPVMLCAAMAPPWGARWESDNTILFGQNSGESQGIFR
                     VSGNGGKPELLVKSGDAGALHGPQMLPGGRAVLFTQIARGAIWDQGQIAVESIDTHTR
                     KVVVNGGTDARYLPTGHIVYVVEGSLFAVPFDVDSLETTGGPVPLVEGIPQAGANFTG
                     AAHFDVAASGSLVYAAGLGSSEARTLVWVDRSGKEEPLGVPPRAYTYPRLSPDGTRVA
                     LDIRDEMRDIWIWDLARKTLTRLTVDSDLTRLPIWIDAQRVAFTVTKDGQAGSSIRVQ
                     AADGSAPSQSLLDDAKRKWLASAASPDGTRILVSPDAAPYDIGVFTIGATPSVTMLLG
                     APSNEVNGEVSPDGRWLAFQSNETGRGEIYVRPFPEVSGSRAQVSTDGGVRPAWSRDG
                     REMFYLWQGAMMAVPVQTTPSFKAGTPQVLFKGDYLAPQNGRTYDVSPDGKRFVMIKA
                     GPETEAPVEASIVVVQNWLEELKRRVPIP"
BASE COUNT          700 a         1549 c         1515 g          745 t
ORIGIN      
        1 cctgttctgc gagtacgtcg tcgggtcgcc gcgcgggcgc cggccgcgga aggaactcgt
       61 gcacaagcgt gacgagctgg tggccacact gaagaaagag gcgaaactca aaactcaaaa
      121 ctcaaaagtc acaactgagt tgtgaattca gtttgagtct tgagtttcga gtcttgagtg
      181 tcgagccgaa tgggaacatt ggcttccgaa ctcggcggcc acgcggagcg cgtatcgggg
      241 catccgcgcg tgtacgtgga cgcgaacgtg cctgcgggcc tcgtctccta catgcggcag
      301 acgctgcact gggacgtcct gttcgtgctc gagcacgacg atctccggcg cgcgcgcgac
      361 ggcgagcact tccggcgcgc gaagcacctt cgccgcacgc tgatcacgct ggaccgcgac
      421 tacctggatg accgtcgatt tcccccctcg gagagcggag gcgtgctggt gctgaccgca
      481 ccggccgagc gcggccttgc cgcgctgctc aagcggctgg atcgcgagat gctgcgccgg
      541 acggcgccct ctgaccgtgg ggacgtcgag gtgctcgcac ctgatccgct cccgctcgaa
      601 ggccggaagc tccacgtgca tgtggactgg agcagcgagg ccgtcgcgcc gtgatcgtcg
      661 tcagcggcgg cgacctcgtg ctgccgggcc gcatcgtgac ggaaggcgcg ctggtcatcg
      721 acggggagcg catcgtcgct atcgagccgc atcgcgtcga cccggccgac gcgaaggtcg
      781 ttgacgccac cgactgctac gtcgtgcccg gcttcatcga cgtgcacgtc cacggccttc
      841 acggacacga cacgctggac gatggggacg cggtggcccg catcgccgcg cacatgcctc
      901 gatacggcgt gacggcgttc tgtcccacga cggtcgcctg tcctccggca gcgctgcgac
      961 gcgtcctgca gcaggtccgg cagttgcggt caacgccgtc cgcaggcagc gcccgcgtgc
     1021 tgcccgcgca cctcgagagc aacttcatca accccgagta ccggggcgca cagcccgcgg
     1081 cgtgcctgcg cacgccggca ggcacacaca aggacggcga gttcgccggc cgcgaggtgc
     1141 tcgacgtcat cgccgcgtcg cggcccgacg tgggcatcgt gacgctcgct cccgaacttc
     1201 ccggcggaat cgatctggtg cgcgcgctcg tcgcggcggg ccaccgtgtg tcgctcggac
     1261 actcgggggc ggatctcgac gtggcgttcg ccgccattga cgcgggtgcc cgacacgcga
     1321 cgcatctctt caaccgcatg acgccgatga cgcatcgcgc tcccggcctg cctggcgctg
     1381 tgctcgcgcg agacgaagtc gccgtcgagt tgatctgcga cggcgtacac gtccatccgg
     1441 ccatgtgcca actggccatg gccgcgaagg gtccgcaggg cgtgatggcg atcaccgacg
     1501 ccacctctgg cgcaggcctg cctcagggct cggcgtcgcg cctcggaggg cagacgatcc
     1561 acgtgcgcga tggcgccgcg ttcctggacg aggggacgct ggccggcagc acgctcacga
     1621 tggaccgcgc gttccgccac atcgtgtccc gcttcggcgg atcgatcgtg gacgccgcga
     1681 tcatgtgttc aacgacaccc gctcgcgggc tcgggctgac cggattcggc gtgctggccg
     1741 atggcgccgt ggcggacctc gtcgtcctcg atcgtggatt tcacgtcgtg cggacgttca
     1801 tcgccggcgt cgaggtgtgg cgtacgtagt gtccggcttc agccggaccg ttacggaatc
     1861 ggcacccgcc tcttcaactc ctcgagccag ttctgcacca cgacgatgga tgcctcgacc
     1921 ggtgcctcgg tctcaggacc ggccttgatc atcacgaacc tcttgccgtc cggcgacacg
     1981 tcgtacgtgc ggccgttctg cggtgcgagg tagtcgccct tgaacagaac ctgcggcgtg
     2041 ccggccttga agctgggcgt cgtctggacg ggcaccgcca tcatcgcgcc ctgccagaga
     2101 tagaacatct cacggccgtc acgcgaccac gctggccgca cgccgccgtc cgtggatacc
     2161 tgcgcgcggc ttccgctgac ctccgggaac ggacgaacgt agatctcgcc tcgccccgtc
     2221 tcgttcgact ggaacgcgag ccaccggcca tctggtgaga cctcgccgtt gacctcgttg
     2281 gacggcgccc cgagcagcat cgtgacgctt ggcgtggcgc caatcgtgaa gacaccgatg
     2341 tcgtagggcg cggcgtccgg tgaaacgagg atgcgtgtgc cgtccggcga cgccgcactg
     2401 gcgagccact tcctcttcgc gtcgtccaac agggactgcg acggcgccga tccatccgcc
     2461 gcctgaacgc ggatggacga gcccgcctgc ccatccttgg tgacggtgaa cgccacccgt
     2521 tgcgcgtcga tccagatggg aagccgggtg agatcggagt cgacggtcag acgcgtcagg
     2581 gtcttacgcg ccaggtccca gatccagatg tcccgcatct cgtcgcggat gtcgagcgcc
     2641 acgcgtgtgc cgtcgggcga gagtctcgga taggtgtacg cgcgcggtgg cacacccagc
     2701 ggctcctcct tcccgcttcg atcgacccag acgagcgtgc gcgcctccga gctcccgagg
     2761 cccgctgcat acacgaggct cccgctggcg gcgacgtcga agtgcgcggc tcccgtgaag
     2821 ttggctcctg cctgcgggat cccctcgacg agtgggaccg gacctccggt ggtctcgagg
     2881 gaatcgacat cgaagggcac ggcaaacagc gaaccctcga cgacgtacac gatgtggccg
     2941 gtgggcaggt agcgcgcgtc ggtgcctccg ttcacgacga ccttgcgggt gtgggtgtcg
     3001 atcgattcga ccgcgatttg cccctggtcc catatggcac cgcgcgcgat ttgcgtaaag
     3061 agcaccgcgc gcccgccggg aagcatctgc ggaccgtgca atgccccggc gtcaccggac
     3121 ttgaccagca actccggctt gccgccgttt ccggagaccc ggaagatccc ctgactttcg
     3181 ccggagttct gaccgaacag gatcgtgttg tccgattccc atcgcgcgcc ccacggcggc
     3241 gccatggccg cgcacagcat caccggagcg ccgccgctga cgggcacctt cttgatctcg
     3301 ccgtcctgcc agaaccccag ccactggccg tcgggcgaga agaaggggtt gcgtccagac
     3361 ccgtttccct cgcccgctgt tccgcgcacc ggcacgggct cgagctgctc gatggcacgc
     3421 atgaagacct ggttgttcgc ggagtaggcg atgcgcgtgc cgtccggtga aacggccacg
     3481 aggtggcggc ccgtgttggt gaatcgctga ccctcgggca gggccaccga gaaacgcgca
     3541 agcggccgaa ccggttcggg ccgaagcgtc cagactgcgt agctggcaac gcctcctccg
     3601 gcaatgagcg cggcggcgta cgtcagcgcg ctgcgccaga tcggaggccg ggcagcaggc
     3661 gcagctttct cggctgctgg ttgctccaga gcgcgctcga gctcatagcg aacgtctccg
     3721 atgtcgcgga ggcgtcggtt cggatccttc gcgagacacc gttccagcag cgtgcgcagt
     3781 ctcggagtcg ccgcagccgg cagcagcgac cactccggct cgcctcgcac gacggcggca
     3841 atcatctctg tagcatccgt cccatcgaac gtgcgcttgc ccgtcagcat ctcgaacagg
     3901 acgcagccga acgcccagac gtcaacgcgc ttgtccacgg cccttccgcg tgcctgctcg
     3961 ggcgccatgt aggcggcggt gccgagaatc acgcccgcgc cagtgaaggc ggcgctcatt
     4021 gtgggagaca tcgacggaat cgacgtgggc gattgaaggc ctgcgctcgg attttcggtg
     4081 aacttcgcga gcccgaagtc gaggaccttg accgttccgt cctcgcgtac cttgatgttg
     4141 gcgggcttga gatcgcgatg aacgatcccg tgcaggtgcg cggtgtcgag cgcgtcggca
     4201 atctgcctgg cgagggcgat cgcgtcgctg acgggaatcg ccccttccac gatccggtcg
     4261 gccagcgtgg gtccctcgat gagctcgagg atgagcgccc gtccacgagg tcccgcgtcg
     4321 gccgggaggt cttccaatcc gtagatgtgc ccgatattgg gatgggtcag cgaggccagc
     4381 acctgggcct cgcgctcgag gcgcgccagc cgttccgggt ccgaggcaaa cagatccggc
     4441 aggaccttga ccgcgacatc ccgctggagg cgcgagtcgc gcgcgcggta cacttcgccc
     4501 atgccgccg
//
LOCUS       DBNGSH010000361         2614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2747633, whole genome shotgun sequence.
ACCESSION   DBNGSH010000361 DBNGSH010000000
VERSION     DBNGSH010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2747633"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="AC16CY_08500"
     CDS             <1..462
                     /locus_tag="AC16CY_08500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3353866.1"
                     /translation="KSCYYRTYCVGKEPTDEQKEVYATALKWLYDSIKAVKAGTTTRE
                     IASKWPSAKEAYGYEEEDQAAANLWGHGLGLAQYDPPVISRIWSLDHPIEIKPGMVFA
                     LETQHGKMYRWGVRIEEMLIVHPDRIEIISNFPVEQITVVDPMPGYGAHVR"
     gene            490..1794
                     /locus_tag="AC16CY_08505"
     CDS             490..1794
                     /locus_tag="AC16CY_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3353867.1"
                     /translation="MRSVNDTSHLLLPLSSVEATDATRFGPKAANLAALGRAGLRIPD
                     GFCLGAEAYRRQVAALGLEETARAVFAGDNPSEAKQHALRMRLGLLEEPIAPAVLEGL
                     LEAWRDLTDRTGALTVVRSSALVEDRFGSSFAGQFESYLGLEQETEFLTAVRACWAAL
                     WSTRALRYMATHNLDPADTAMAVLVQPLVVAVAAGGGLSRTADGGMLVNATMGLGASV
                     AQGEVTPDRFELDRGGSLLHVAPGRKDHALRCGHRRAPTAEAVPAALAEQPCLDEAQV
                     SELARLLRRAEDVMGMPVEIEWALDEDGFTLLQARPLHLQAPHVPDEIWLQHPRLNGH
                     PAGIGWGAGRAVVINCECELGRIAPGDVLITRVAGPALGHILRQVAGVVTERGGSTSH
                     FASLARERGIPMVLGVADATRRIPDGAQVAVDGVAGIVRWMS"
     gene            1820..2125
                     /locus_tag="AC16CY_08510"
     CDS             1820..2125
                     /locus_tag="AC16CY_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIR3353868.1"
                     /translation="MVFTKRLREGVRRGRIRCSIRIWTKPHVKAGGRYPMDEGQIVVD
                     SIVPIRLKDITQELARESGFASKKDLLEMARHGSGRNVYLIRFHYLPPGGWDDILAP"
     gene            2122..>2614
                     /locus_tag="AC16CY_08515"
     CDS             2122..>2614
                     /locus_tag="AC16CY_08515"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3353869.1"
                     /translation="MKARLSAVLSVAVLCSIPAPGQQLQSVTLNYPTRSGASWPLFIA
                     KEGGYYQKYGLDATLVFAGHPAGIAMVVSDQAHMSSYNLESVMQASSRGDAPFTVVGA
                     SLNKAYFALMARKEIASVKDLKGKTLAVSQIGDPPYNYTSAFLRKFGLTPRDVQWIAA
                     GADA"
BASE COUNT          443 a          859 c          906 g          406 t
ORIGIN      
        1 aagtcctgct actaccggac ctactgcgtg ggcaaggagc cgaccgacga gcagaaggag
       61 gtctacgcga ccgcgctcaa gtggctgtac gactcgatca aggcggtgaa ggcgggcacg
      121 acgacgaggg agatcgcctc gaagtggccg tcggcgaaag aggcgtacgg ctacgaagaa
      181 gaggatcagg cggcggccaa cctgtggggc cacgggttgg gcctcgccca gtacgatccg
      241 ccggtgatct cgcgtatctg gtcgctcgat catccgatcg agatcaagcc gggcatggtc
      301 ttcgccctcg agacgcagca cgggaagatg taccgctggg gcgtgcgcat cgaggagatg
      361 ctgatcgtgc acccggatcg gatcgagatc atctcgaact tcccggtcga gcagatcacg
      421 gtggtcgatc caatgcccgg ctacggagcg catgtcaggt gatgctgaac acgaactgag
      481 aagcgagaag tgagaagtgt gaacgacacg tcgcatctgc tgcttccgct ctcgtccgtg
      541 gaagcaacgg atgcgacgcg ctttggcccc aaggccgcga acctcgccgc gctcgggcgc
      601 gcgggactga ggattcccga cggcttctgc ctgggtgccg aggcctatcg gcgtcaggtc
      661 gcggcgctcg gtctggagga gacggcgcgc gcggtcttcg ccggcgacaa tccatccgaa
      721 gcgaagcagc acgcgctccg gatgaggctc ggcctgctcg aagagccgat cgcccccgcg
      781 gtcctcgagg ggctgctcga ggcgtggcgc gatctcaccg accggacggg cgcgttgacg
      841 gtcgtccgct cgtcggcgct cgtggaagac cgcttcgggt cgagcttcgc agggcagttc
      901 gaaagctacc tgggtctgga gcaggagacc gagttcctca cggcggtacg ggcctgctgg
      961 gcggcgctct ggtccactcg ggcgctgcgc tacatggcca cgcacaatct cgacccggcc
     1021 gacaccgcca tggccgtcct ggtgcagccg ctcgtggtag ccgtcgctgc cggcggcggt
     1081 ctgagcagaa ccgccgacgg cgggatgctc gtgaacgcga cgatgggcct gggcgcgtcg
     1141 gtcgcgcaag gcgaggtcac gcccgatcgt ttcgagctcg atcgcggcgg atcgctcctc
     1201 cacgtggctc cgggccgcaa ggatcacgcg ctccgctgcg gccatcgccg cgcgccgacc
     1261 gccgaggccg tgcccgccgc gctcgccgag cagccgtgcc tcgacgaagc acaggtctcc
     1321 gagctcgcgc ggctgctgcg cagggccgaa gacgtgatgg gcatgcccgt cgagatcgaa
     1381 tgggccctgg acgaggacgg cttcacgctg ctgcaggcgc gtccgctgca cctgcaggcg
     1441 ccgcacgtac ccgacgagat ctggctgcag cacccgcgtc tcaacggcca tcccgccggc
     1501 atcggctggg gcgcgggccg ggccgtcgtc atcaactgcg aatgcgagct cggacggatt
     1561 gcccctggcg acgtactgat cacgagagtt gccggtccgg cgctcggcca catcctgcgt
     1621 caggtcgccg gcgtggtgac cgaacgcgga ggcagcacgt cgcacttcgc ctcgctcgcg
     1681 cgcgaacggg ggatcccgat ggtgctcggc gtcgccgacg ccacgcgtcg aatcccggac
     1741 ggcgctcaag ttgccgtcga cggcgtggcc ggcatcgtcc gctggatgtc ctgaagctct
     1801 cggctccggg ctttcgccaa tggtgttcac caagcggctg cgcgaggggg tccgacgcgg
     1861 caggatcagg tgcagcattc gcatctggac gaaaccccat gtgaaggccg gcggccgcta
     1921 cccgatggac gagggacaga tcgtcgtcga ttcaatcgtg ccgatcaggc tcaaggacat
     1981 cacgcaggaa ctggcgcgcg aatcgggctt cgccagcaag aaggacctgc tggagatggc
     2041 acggcacggc agcggccgca acgtctacct gattcgattt cactacctgc cgcccggcgg
     2101 ctgggacgat atactcgccc catgaaagcc cgcctgtcgg ctgtcctctc cgtcgccgtg
     2161 ctttgcagca ttcccgcccc tggccagcag ctccagagcg tcacgctgaa ctaccccacc
     2221 cgaagcggcg cctcgtggcc gctcttcatc gcgaaggagg ggggctacta ccagaagtac
     2281 gggctggacg cgacgctggt gtttgccggg cacccggccg ggatcgcgat ggtcgtcagc
     2341 gatcaggcgc acatgagcag ctacaacctg gagtcggtga tgcaggccag ctccagggga
     2401 gacgcgccgt tcacggtcgt cggcgcctcg ctgaacaagg cgtacttcgc gctgatggcg
     2461 cggaaggaga tcgccagcgt caaggacctc aaggggaaga cgcttgcggt cagccagatt
     2521 ggcgatcctc cctacaacta cacgagcgcg tttcttcgca agttcgggct gacgccgcgc
     2581 gacgtgcaat ggatcgccgc cggcgccgac gcga
//
LOCUS       DBNGSH010000362         3018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2407999, whole genome shotgun sequence.
ACCESSION   DBNGSH010000362 DBNGSH010000000
VERSION     DBNGSH010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2407999"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="AC16CY_08520"
     CDS             <1..1431
                     /locus_tag="AC16CY_08520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR3353870.1"
                     /translation="HVLPRFDGPVYGTALTLAFVEPKLQEHGIDARDRFQVVAPRQRV
                     TVGPLSIEFIRVTHSMPDCVALAITTPIGTLIHTGDFKIDQTPIDGQHFDLHRFAELG
                     SEGVLALFADSTNIDRPGFTGPETDVVDAFEELFTSTQGKLVVAAFSSSIYRMQVLVD
                     LAAQFERKVAFVGRSVVQNSEIAQRLGYLRIPSGMQIRDVDVGNYASQDVLCITTGSQ
                     GEPQAALPRIAIDDHRYVKLGPDDTVVFSARSIPGNEKAIARTMSHVARRGANVVTPE
                     MKHVHVSGHGSAEELKLMLSLVRPRYFVPIHGEYRQLSQHARVASRVLRGAGRNVQVL
                     LAQDGDIIQFDAHGARIADKAPTGRVLIDTTRVGEVGDEVLRDRQHLAVDGVVVAVVA
                     ISRHTGAMTGEPELVARGFVVNEGEEESLFSDAARTIGDCIESAGVEERTDQGLMTEK
                     LRVELRRFFKKRSGRRPLVLPVLMEI"
     gene            1433..>3018
                     /locus_tag="AC16CY_08525"
     CDS             1433..>3018
                     /locus_tag="AC16CY_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR3353871.1"
                     /translation="MTESKLSRRISEFVGVALFALTLIWLISLVTHNPSDPVWFFTTG
                     AGHPPVNFAGRVGAFLSELSFQLFGYAAFLIPAVLGVAGWHYFWCQSPDAPYTKLTGI
                     SLLLVGSSAVLSLLFADAGASGKTFYAGGSAGSWVGGLLSEYLNRTGSLIVLLTVIVL
                     AVILSTQFSFGRMFATASVGSRTLSARGAGWLRGWIDQRRREKARRAVIAKQTRKASG
                     ADDDADAAAEDASSRQSKAVRASSADPAAVPGPRPAPVVSRKRAEAAQPLPLPEPEQK
                     APAPRRQGAFTLPPATLLDSPKVERKIDERELMESARLLEDKCREFAVEGQVAQIHPG
                     PVVTTFEFKPEAGVKYSKVTGLADDLCLAMKAESVLIDRIPGKSTVGIQIPNHVREPI
                     SLRELLESEAYQRSQSKLTLVLGKTIHGEPFVTDLAMMPHLLIAGSTGTGKSVGLNGM
                     LTSLLYRATPDDVRLILIDPKRLELGMYEEIPHLLTPVVVDPKKAANALRWAVREMEE
                     RYKTLAAEGVRNIEQYNRNAR"
BASE COUNT          499 a         1030 c          990 g          499 t
ORIGIN      
        1 cacgtgctgc cccgattcga cggacctgtg tacggaacgg cgctcacgct cgctttcgtc
       61 gagcccaagc tccaggagca cggcatcgac gcgcgagacc ggtttcaggt ggtcgcgcca
      121 cggcagcggg tcaccgtcgg gccgctctcc atcgagttca tccgcgtgac gcacagcatg
      181 ccagactgcg tggcgctggc catcacgacg ccgatcggca cgctcatcca cacgggcgac
      241 ttcaagatcg atcagacgcc catcgacggg cagcacttcg acctccatcg attcgccgaa
      301 ctgggctcgg aaggcgtcct ggcgctcttt gccgacagca cgaacatcga tcggcccggc
      361 ttcaccgggc cggaaaccga cgtcgtggac gccttcgagg aactgttcac cagcacgcag
      421 ggcaagctcg tggtcgcggc cttctcgtcg agcatctatc gcatgcaggt cctcgtggac
      481 ctggcggcgc aattcgaacg aaaggtggcg tttgtcggcc ggagcgtcgt ccagaactcg
      541 gagatcgccc agcgtctcgg gtacctgcgc atcccgtccg gcatgcagat ccgcgacgtg
      601 gacgtcggca actacgcgtc gcaggacgtg ctgtgcatca cgaccggctc ccagggcgag
      661 ccccaagcgg cgctgccgcg gattgccatc gacgatcatc gctacgtcaa gctcgggccc
      721 gacgacaccg tcgtcttctc cgcccggtcg attcccggca acgagaaagc gatcgcccgg
      781 acgatgagcc acgttgcccg gcgtggcgcc aacgttgtca ccccggagat gaagcacgtt
      841 cacgtgtccg gtcacgggtc ggccgaggag ctgaagctga tgctctcgct tgtgcggccg
      901 cgctacttcg tcccgattca cggcgagtac cggcagctgt cgcagcacgc ccgtgtcgcc
      961 tcccgggtcc tgcggggcgc cggacgcaac gtccaggtcc tgctcgcaca ggacggcgac
     1021 atcatccagt tcgacgcgca cggcgcgcgc atcgccgaca aggcgccgac cgggcgcgtg
     1081 ctgatcgaca ccacgcgggt cggcgaggtc ggagatgaag tgctgcggga ccggcagcat
     1141 ctggccgtgg acggcgtcgt cgtggccgtc gtcgctatca gccgccacac cggggcgatg
     1201 acgggcgaac ctgagctggt cgcacggggg ttcgtcgtga acgagggcga agaagaatcg
     1261 ctcttcagtg acgctgcccg tacaatcgga gactgcatcg aatcggccgg cgtcgaggag
     1321 cgcaccgacc agggactgat gaccgagaag ctgcgcgtcg agctgcggcg gttcttcaag
     1381 aagcggtcgg gccgccggcc gctcgtcctg cccgtgctca tggagatctg aggtgacaga
     1441 gtccaagctc tcgagacgga tcagcgagtt tgtcggcgtc gcgttgttcg cgctcacgct
     1501 gatctggctg atttccctcg tcacgcacaa cccgagcgat cccgtgtggt tcttcacgac
     1561 gggcgccggg cacccgccgg tcaacttcgc cggccgcgtc ggagcgttcc tgtccgagct
     1621 gtcgttccag ctgttcggct acgccgcttt cctgattccg gctgtcctcg gcgtcgcggg
     1681 ctggcactat ttctggtgtc agtcgccgga cgctccgtat acgaagctca ccggcatttc
     1741 cctcctgctg gtcggatcga gcgccgtgct gagcctgctc ttcgcggacg cgggcgcgtc
     1801 cggcaagacg ttctacgcgg gcggatcggc cgggtcctgg gtgggcggcc tcctgtcaga
     1861 gtacttgaac cggaccggct ccctcatcgt gttgttgacg gtgatcgtgc tggccgtgat
     1921 cctctccacg cagttctcgt tcggccggat gtttgccacc gcttcggtcg gctcgcggac
     1981 gctgtcggcg cggggcgcgg gctggcttcg cggctggatc gaccagcgcc gccgtgagaa
     2041 ggcgcgccgc gctgtcatcg cgaaacagac ccgcaaggcc tcgggcgccg acgacgacgc
     2101 tgatgcggcg gccgaagacg cttcctcgag acagtccaag gcggtgcgcg cgtcgtcggc
     2161 ggatccggcc gccgtgcctg gcccgcgacc ggcacccgtg gtgtcacgaa agcgcgccga
     2221 agcggcccag ccgcttccgc tccccgaacc agagcagaag gctccggcac cgcgccgcca
     2281 gggtgccttc acgcttccgc cggccacgct gctcgactcg ccgaaggtcg agcggaagat
     2341 cgacgagcgc gagctgatgg aatcggcgcg gctgctcgag gacaagtgcc gcgagttcgc
     2401 cgtcgaggga caagtcgccc agattcatcc gggacccgtc gtcacaacgt tcgagttcaa
     2461 gcccgaggcc ggcgtgaagt acagcaaggt caccggtctc gccgacgacc tctgtctcgc
     2521 catgaaggcg gagtcggtgc tgatcgatcg cattcccggc aagtccacgg tcggcatcca
     2581 gatcccgaac cacgtgcgcg agccgatctc gctccgtgaa ctgctcgagt ccgaagcgta
     2641 ccagcgatcg cagtcgaagc tgacactcgt gctcggcaag acaatccacg gcgagccctt
     2701 cgtgaccgac ctcgcgatga tgccgcatct gctcattgcg ggctccacgg gcaccggcaa
     2761 atcggtcggg ctcaacggca tgctgacaag cctgctctat cgcgcgacgc ccgacgacgt
     2821 gcggctgatc ctcatcgacc ccaagcgcct cgagctcggg atgtacgagg aaatcccgca
     2881 cctgctgacg cccgtcgtcg tggatcccaa gaaggccgcc aacgcactcc gctgggccgt
     2941 gcgcgagatg gaagaacggt acaagacgct ggccgcggaa ggggtccgca acatcgagca
     3001 gtacaaccgg aacgcgcg
//
LOCUS       DBNGSH010000363         2704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1050025, whole genome shotgun sequence.
ACCESSION   DBNGSH010000363 DBNGSH010000000
VERSION     DBNGSH010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1050025"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="AC16CY_08530"
     CDS             complement(<1..156)
                     /locus_tag="AC16CY_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HIR3353872.1"
                     /translation="MAIADYPSRIVCLTEETTETLYLLGEGHRVVGVSGYTVRPPEAR
                     RKPRVSAF"
     gene            complement(181..852)
                     /locus_tag="AC16CY_08535"
     CDS             complement(181..852)
                     /locus_tag="AC16CY_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dph6-related ATP pyrophosphatase"
                     /protein_id="HIR3353873.1"
                     /translation="MKILLSWSSGKDSAWALHLMHQRYPGAVGGLLTTMNEAVDRVAM
                     HAVRRDVLSAQARASGLPLRVVPIPHPCPNEVYEERMRAAVAWAAGDGFTHIAFGDLF
                     LEDVRRYREERLAGTGLEPLFPVWGLPTRQLAEAMIDRGLQARISCVDTRVLDRTFAG
                     RAFDRSLLEDLPPRVDPCGENGEFHTCVHAGPMFGEPFALEVGETVEREPFVWKDLKL
                     STAES"
     gene            complement(849..1751)
                     /locus_tag="AC16CY_08540"
     CDS             complement(849..1751)
                     /locus_tag="AC16CY_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3353874.1"
                     /translation="MAARSKELVARSLPRTALLAAVLLAFLAAAAGAQSQRAGARRIV
                     SLVPALTEALFAIGAGPQVVGVSSFDEFPPEVKTRPRVGALLDPDIERIFTLRPDLVI
                     VYGSQSDLQSQLSRAGIRAFNYRHGGIQTTLDTIRQLGAATSHESEAEGLISSLHRRL
                     DAVRARVKGRARPRTLLVLERQPGTLREVYASGGVGFLHEMLEIAGGRNVFADAARES
                     VQPSVETLLTSAPDVILEVRATAVTEPRAAAERQAWASLASVPAVRQQRVYLLSGDYL
                     VVPGPRLAEATEAFARTLHPDAFK"
     gene            complement(1727..>2704)
                     /locus_tag="AC16CY_08545"
     CDS             complement(1727..>2704)
                     /locus_tag="AC16CY_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR3353875.1"
                     /translation="TTVLGFGTLAVATCLLAPLVGTTSISLARAFDRSIPIEQNVDAQ
                     IFFVARLPRVLAGALVGSALASAGVVFQAILRNPLATPFTLGVSAGASLGAMLVIVFG
                     GALALGPFSTISAASLAGAAAATAMVYWLSTRQGHAMSTSVLLLAGVTLNSFFSALIM
                     FVQYLADFSQVYRAARWIMGDLDVGGLDTLYASLPLVVAAFALFALLPSSLNLLSLGA
                     DAAAARGVDVARAQRIAFFSASLATAAAVSLAGPIGFIGIVVPHLVRLIVGVDHRIVL
                     PASALFGAAFLVACDLLARVVLAPIEIPVGVVTAMIGGPFFLWLLVRKS"
BASE COUNT          500 a          964 c          901 g          339 t
ORIGIN      
        1 gaacgccgag acgcgcggtt tccggcgggc ctccggaggc cgcaccgtgt agcccgagac
       61 gccgaccaca cgatgccctt cgccgagaag atagagcgtc tcggtggtct cttccgtcag
      121 acacacgatg cgtgaagggt aatcagcaat cgccattcaa caatcaccaa cgttcaacaa
      181 tcacgattcg gcagtcgaca atttcaggtc cttccacacg aacggctcgc gctcgacggt
      241 ctcgccgacc tcgagcgcga agggctcgcc gaacatcggc cccgcgtgaa cgcaggtgtg
      301 gaactcgccg ttctcgccgc acggatcgac gcgcgggggc aggtcctcca gcagcgatcg
      361 gtcgaacgcg cgccccgcga acgtgcggtc gaggacgcgc gtgtccacgc aggagatccg
      421 cgcctgcagc ccgcgatcga tcatcgcctc ggcgagctgt ctcgtcggga gaccccacac
      481 cgggaacaat ggctcgaggc cggtccccgc cagccgctcc tcgcggtaac gccggacgtc
      541 ctcgaggaac aggtcgccga acgcgatgtg cgtgaacccg tcgccggccg cccaggccac
      601 agccgctcgc atccgctctt cgtagacctc gttcggacat ggatgcggga tcggcacgac
      661 acgaagcggc agcccggacg cgcgggcctg tgcgctgagc acgtcgcgcc ggaccgcatg
      721 catcgcgacg cgatcgacgg cctcgttcat cgtcgtcagc agtccgccca ccgcaccggg
      781 ataccgctgg tgcatcaggt gaagcgccca tgcgctgtcc ttgcccgagc tccacgacaa
      841 cagaatcttc atttgaacgc gtccggatgc agcgtgcgcg cgaacgcctc ggtcgcttcg
      901 gccagccgcg gcccgggaac gaccaggtag tcgccagaca ggagatagac gcgctgctgc
      961 ctcaccgctg gcaccgaggc caggctcgcc cacgcctgcc gctctgcggc ggcgcgcggc
     1021 tccgtgacgg ccgtcgcccg cacttccagg atcacatcgg gtgcgctcgt cagcagcgtc
     1081 tccaccgacg gctgcaccga ctcgcgcgcg gcgtccgcga acacgttacg gccgcctgcg
     1141 atctccagca tctcgtggag gaagccgacg ccgccgctcg cgtagacctc gcgcagcgtt
     1201 ccgggctggc gctcgagcac gagcagcgtt cgcgggcgcg cgcgcccctt cactctggcg
     1261 cgcaccgcat cgagtctcct gtgaagcgac gagatcagcc cctcggcctc ggactcgtgc
     1321 gacgtcgcgg cgcccagttg gcgaatcgtg tcgagcgtcg tctgaatccc tccgtggcga
     1381 tagttgaacg cccgaattcc cgcacgagac aactgcgact gcagatccga ctgcgagccg
     1441 tacacgatca ccaggtccgg ccgcagcgtg aagatccgct cgatgtccgg gtcgagcagc
     1501 gcaccgactc tggggcgcgt cttcacttcc ggcggaaact cgtcgaagct gctgacgccg
     1561 accacctggg gtccggcgcc aatcgcaaag agcgcctcgg tcaacgccgg cacgagcgac
     1621 acgatgcgcc gggcgcccgc acgctggctc tgcgcccctg ccgcggcggc gaggaacgcc
     1681 agcagaaccg cggccagcag tgcggtccgc ggcagcgagc gcgcgactag ctctttcgaa
     1741 cgagcagcca caggaagaac ggacctccaa tcatggcggt gacgacgccg accgggatct
     1801 cgatcggcgc gagcaccacg cgggccagca aatcgcacgc gacgagaaac gccgcgccga
     1861 agagcgccga cgccgggagc acgattcgat gatcgacgcc aacgatgagg cgaaccaggt
     1921 gcgggacgac gatgccgatg aagccgatcg ggccggcaag cgagacggcg gcggccgtgg
     1981 ccagcgaggc gctgaagaag gcgatgcgct gcgcgcgcgc cacgtccacg ccgcgtgccg
     2041 cggccgcgtc ggcgccgagg ctcagcagat tgagcgacga gggcagcagt gcgaacagcg
     2101 cgaaagccgc caccacgagc ggcagcgacg cgtacagcgt gtcgagaccg cccacgtcca
     2161 ggtcgcccat gatccatcgc gcggcgcggt agacctgcga gaagtccgcg aggtactgaa
     2221 cgaacatgat gagcgccgag aagaacgagt tgagcgtgac gccggccagc agcagcaccg
     2281 aggtggacat cgcgtgcccc tgccgcgtcg agagccagta gaccattgcc gtcgccgcgg
     2341 cggcgcccgc gaggctcgcg gcagaaatcg tcgagaaggg gccgagcgcg agcgcgccgc
     2401 cgaagacaat gacgagcatc gctccgagcg acgcgccggc cgacacgccc agcgtgaacg
     2461 gtgtggccag cgggttccgg aggatcgcct gaaagacgac tccggccgac gcgagcgccg
     2521 atcccacgag cgctcccgcg agaacccgcg gcagtcgcgc gacaaagaag atctgcgcgt
     2581 ccacgttctg ctcgatcgga atcgaccgat cgaacgcgcg ggccaggctg atcgacgtag
     2641 tcccgacgag cggcgccagc aggcaggtcg cgaccgccag cgtcccgaag cccagcaccg
     2701 tggt
//
LOCUS       DBNGSH010000364         1916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3087977, whole genome shotgun sequence.
ACCESSION   DBNGSH010000364 DBNGSH010000000
VERSION     DBNGSH010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3087977"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC16CY_08550"
     CDS             complement(<1..220)
                     /locus_tag="AC16CY_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR3353876.1"
                     /translation="MTDRIYYTDSECRSFDATVTRPVERDGRAAVVLDRTAFYPTSGG
                     QPFDQGRLNCLAPSHLRTLTLDVVETIDE"
     gene            complement(217..888)
                     /gene="queC"
                     /locus_tag="AC16CY_08555"
     CDS             complement(217..888)
                     /gene="queC"
                     /locus_tag="AC16CY_08555"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR3353877.1"
                     /translation="MADAVVLLSGGLDSYTAAAIARADGFRLHALTIKYGQRHAAEIV
                     AARAVAAWLGVARHVEVDVGLDAFGGSSLTTDTPVPKDRPLDSKEIPSTYVPARNTVF
                     LALALGWAEVLGVRDIVIGVNALDYSGYPDCRPEFIAAFEQLASLATARGVHGERFRI
                     HAPLQMMTKAEIVRRGTALGLDYGLTHSCYDPGPAGPCGHCDSCVLRAAGFAEAGLAD
                     PGLRR"
     gene            complement(920..1561)
                     /locus_tag="AC16CY_08560"
     CDS             complement(920..1561)
                     /locus_tag="AC16CY_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3353878.1"
                     /translation="MLTVNEIFYSVQGESTYAGRPCVFVRLTACDLRCSWCDTEYAFH
                     EGRKREVDEVLDDVDRYECPLVEVTGGEPLLQESVYPLMEGLLARGKTVLLETGGHRS
                     TAHVPADVVTILDVKCPGSGESARNCWENLERLRPRDEVKFVVKDRADYEFARDVVCS
                     RALGARAAAIHFSPVHGVLDPKTLSEWVLSDRLPVRVQLQLHKYIWSPTTRGV"
     gene            1639..>1916
                     /locus_tag="AC16CY_08565"
     CDS             1639..>1916
                     /locus_tag="AC16CY_08565"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR3353879.1"
                     /translation="MALRIGTSGWNYPSGKGTWNGVFYPATRSKRQGTADFDELRFYA
                     EHFDTVEVNTTFYGQPKPDVTRGWVSRTPPGFEFSLKLYQKFTHPRMFR"
BASE COUNT          338 a          625 c          643 g          310 t
ORIGIN      
        1 cttcgtcgat cgtctcgacg acatccagag tgagggtgcg aaggtgcgaa ggtgcgaggc
       61 aattgagccg gccctggtcg aagggctggc cgccggaggt cgggtagaag gccgtgcgat
      121 cgagcacgac cgccgctcgc ccgtcgcgct cgaccgggcg cgtcaccgtc gcgtcgaacg
      181 agcggcattc cgagtctgtg taatagatgc gatcggtcat cggcgcagcc ccggatcggc
      241 gaggcctgct tcggcaaagc cagctgcccg cagcacgcag ctgtcgcagt ggccgcacgg
      301 gccggccggg ccgggatcgt agcagctgtg cgtgaggccg tagtcgaggc cgagggccgt
      361 gccgcggcgg acgatctcgg ccttggtcat catctgcagc ggcgcgtgga ttcgaaaccg
      421 ttcgccgtgc acgccgcgag ccgtcgccag ggacgcaagc tgctcgaagg cggcgatgaa
      481 ctcgggccgg cagtccgggt acccggagta atcgagcgca ttcacgccga tcacgatgtc
      541 gcgcacgcca aggacctcgg cccacccgag agcgagcgcc agaaacaccg tgttgcgcgc
      601 gggcacgtag gtggacggaa tctccttcga gtcgagcggc ctgtccttcg ggacgggtgt
      661 gtcggtcgtg agcgaagatc cgccgaaggc gtcaaggccg acatcgactt ccacgtggcg
      721 cgcgacgccg agccatgccg cgacggcacg cgcggcgacg atctcggccg catggcgctg
      781 tccgtacttg atcgtcagcg cgtgcaggcg gaatccgtcg gcccgggcaa tggctgctgc
      841 ggtgtaggag tcgaggccac cgctgagaag aacgactgca tccgccatgt ctcagatcta
      901 accacggggt cagacccctt cacactcctc gcgtggtggg gctccagatg tacttgtgca
      961 actgcagctg cacacggacc ggcagccgat cggaaagcac ccactctgac agcgtcttgg
     1021 ggtcgagcac tccgtgcacg ggcgagaagt gaatggctgc ggcgcgcgcg ccgagtgcgc
     1081 ggctgcagac gacgtcccgg gcgaactcgt agtcggcgcg atccttgacg acgaacttga
     1141 cctcgtctcg cgggcgcagc cgctcgaggt tctcccagca gttgcgcgcg gactctccgc
     1201 tgccagggca cttcacgtcg aggatcgtga ccacgtcggc tggcacgtgc gccgtgctgc
     1261 ggtggccgcc cgtctcgagc aggacggtct tcccgcgcgc gagcaggccc tccatcaacg
     1321 gatagaccga ctcctgcaag aggggctcgc cgccggtgac ttcgacgagc gggcactcgt
     1381 acctgtcaac gtcgtccagg acctcgtcga cctcgcgctt tcgaccttcg tgaaacgcgt
     1441 actccgtgtc gcaccacgag cagcgcaagt cgcacgccgt cagccgcacg aagacgcacg
     1501 gcctgccggc ataggttgac tccccctgca cggagtagaa gatctcgttg acggtcagca
     1561 tgggaaatgg ggtcgggagt catttttctg ccgggaagaa aaatgactcc cgaccccctt
     1621 acagtattgt gtaccgacat ggctcttcgc atcggcacct ccggctggaa ctacccgtcg
     1681 ggcaagggca cgtggaacgg cgtcttctat cccgccaccc ggtccaagcg ccagggtacg
     1741 gcggacttcg acgaactgcg cttctacgcg gagcatttcg acacggtgga agtgaacacc
     1801 acgttctacg gccagccgaa gccggacgtc acgcgaggct gggtcagcag aacgccgccg
     1861 ggcttcgagt tctcgctgaa gctgtaccag aagttcacgc acccacggat gtttcg
//
LOCUS       DBNGSH010000365         5017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2238816, whole genome shotgun sequence.
ACCESSION   DBNGSH010000365 DBNGSH010000000
VERSION     DBNGSH010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2238816"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC16CY_08570"
     CDS             <1..243
                     /locus_tag="AC16CY_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HIR3353880.1"
                     /translation="WLQDRGVRFVETPHEEFDTMGTNVLALGPSRCLTLAGNPLTRRA
                     LERAGCEVMEYQGFEISVKGAGGPTCLTRPLVRDTH"
     gene            352..615
                     /locus_tag="AC16CY_08575"
     CDS             352..615
                     /locus_tag="AC16CY_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009278376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353881.1"
                     /translation="MGKFFTAVKRGFQSMGEAPTGERYTVAGKAVACLHCSHDRFVEG
                     RAQLNTAGLTFFNLDWANRSAATLTCTSCGRIEWYLTDPEEVG"
     gene            complement(676..2163)
                     /locus_tag="AC16CY_08580"
     CDS             complement(676..2163)
                     /locus_tag="AC16CY_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3353882.1"
                     /translation="MLLRTFTVAALVALLTAGTAAQNAGSVVAAASKAMGVDTLTSIT
                     YSGTARNGAFGQSKSIGDPMGPVNLTRITEYTRTINFAPPGDPAALVSRATGPTQPPT
                     VPGVAPPMPGVFNQNVTGAQASNNWNQALNIWTTPWGFLKGAAANNATVRQQGGQQLV
                     SFTPPNLKSPSGQTYSVTGYISGQNLVTKVETRVDHAVVGDLLVEFEYSNYQNMNGVQ
                     VPTRIVQKQAGLQTFDAEIKSATPNPPNLTALLTPPAPPAGRGGGPGGAPGGPPPAAA
                     GPAFEKIGDGAFKIGGNYTSLAVDMGDHVLVVESGQNDARGLAVMAAAKQAVPNKPIR
                     FVVNSHPHFDHASGLAAAVAEGATILTHNNNEQVLERLLSGPRTLIGDSLSKVTTRRT
                     DVVRGVGDRDVRKGTNGKVVELHRVPNEHSDGMLMVYLPAEKVVWSADITVVNPNPAQ
                     LGVVKSAADALGKLGLDYNGWIPAHPPQPDRPLTKADVTAALAAK"
     gene            2464..2844
                     /locus_tag="AC16CY_08585"
     CDS             2464..2844
                     /locus_tag="AC16CY_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353883.1"
                     /translation="MSHRTFLTAAALVLGLAVGLVAAQDKAQTKTAVGAVAKIDGTNL
                     SVDTGKGVTLQFVTSTTTGVKVATGSSQAREAKAEGKAGVKITDAVHVGDQVQVRYTD
                     VGGKLMASEVEVLQRRPASGLPVK"
     gene            complement(2892..4079)
                     /locus_tag="AC16CY_08590"
     CDS             complement(2892..4079)
                     /locus_tag="AC16CY_08590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3353884.1"
                     /translation="MAGAGIAGVAAAHQLLVRDGVRRVALVDPREPLSLTSSKGTEAY
                     RNYWPGPDDTMARFMNRSIDLLEALDRDSGRAFELNRRGYVFLTATLAESERLRAHEG
                     PAMEFLEGEDAIHARFPFVNERARAMLHVRRAGYMNALKLGAYLLERARAGGLDFVRD
                     EVTRLVTSDQRLVAVELASGARIDARALVFAAGPLLPEWVERLGLGVPILNELHGKIS
                     FEDEDGLIPRDAPLMIWNDPVDLGARGTFPPGVHLRPRGARSVLGIWTYDTRIETPRF
                     PPVFDGSYGEVVIRGLAEMVPGLSAYVSRAAAAVVDGGYYCKAPDNRPLIGPTSIEGV
                     FLLGALSGFGIMASQAAAELLACHLLDRPRPDYAAAFHPARFADPSYRRVLATLDARS
                     GQL"
     gene            complement(4114..4722)
                     /locus_tag="AC16CY_08595"
     CDS             complement(4114..4722)
                     /locus_tag="AC16CY_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353885.1"
                     /translation="MNSQDSFKHRSVMLALAAMVALASAAAVRAQEDNFFPKTKRWGR
                     AAIEYRDPEIQVVAAYYYSQRNHNTRWLLIQSAMSTTRNLVIDRENITLVTPSGERVI
                     TLSSQQRFASDVSRVNPVVQGAYVLRHDVLSYFLEKRRIESMRLFALESGPVLTNFVS
                     DKDHVITGDLFFESPTGLWTPGTYSLIIEREGTRAVLPIVLE"
BASE COUNT          845 a         1614 c         1714 g          844 t
ORIGIN      
        1 tggctccagg atcgcggtgt gcggttcgtc gaaacgcccc acgaagagtt cgacacgatg
       61 ggtaccaacg tgctcgcgct cgggccgagc cgatgcctga cgctggcggg caatccgctg
      121 acgcgccggg cgctcgaacg cgcaggctgc gaggtgatgg aataccaggg cttcgagatc
      181 agcgtgaaag gcgccggcgg gccgacctgc ctcacgcggc cgctggttcg agacactcac
      241 taggggcggg cgggggcgcg cccgaggaga ttccgcaacc gtcagtcggt tccggccagt
      301 caatactagt gtttgaaccg tcaggcagac caacgatttg agggaggacc agtgggcaag
      361 ttcttcaccg ctgtgaagcg tgggtttcag tcgatgggtg aggctccaac gggcgagcgc
      421 tacacagtcg caggcaaggc cgtcgcgtgt ctacactgct cgcatgaccg attcgtcgag
      481 gggcgcgcac agctcaacac ggcgggattg accttcttca atctcgattg ggccaatcgc
      541 agcgccgcaa cgctgacgtg cacatcgtgc ggccgcatcg agtggtatct caccgatccc
      601 gaggaagtcg gctgagcgga cggccgcctg acaagcgcgg gggcctcagc ccccgcgcgc
      661 atcgatgaga gaacgctact tcgcggcgag agccgccgtg acgtcggcct tcgtcagcgg
      721 ccggtcgggc tggggcgggt gcgcgggaat ccacccgttg taatcgagcc cgagcttacc
      781 gagcgcgtcg gccgccgact tcaccacgcc cagctgggca gggttgggat tgacgacggt
      841 gatgtcggcc gaccacacga ccttttccgc cggcagatag accatcagca tgccgtcgct
      901 gtgctcgttc ggaacccggt gcagctcgac cactttgccg ttggttccct tccgcacgtc
      961 gcgatcgccg acgccccgaa cgacgtcggt ccggcgggtc gtcaccttcg acaggctgtc
     1021 gccgatgagc gtacggggcc cggacagaag acgctcgagg acctgctcgt tgttgttgtg
     1081 cgtcaggatg gtggcgcctt cggccaccgc tgccgcaagg ccgctggcgt gatcgaagtg
     1141 aggatgcgag ttgacgacga accgtatcgg cttgttcgga acggcctgct tggccgcggc
     1201 catgaccgcc aggccccggg cgtcattctg gccgctctcg acgacgagga cgtgatcgcc
     1261 catgtccacg gcgagcgacg tgtagttgcc gccgatcttg aaggcgccgt cgccgatctt
     1321 ctcgaacgcc ggacctgccg ccgccggagg cggcccgccc ggcgcgccgc cggggccgcc
     1381 gccacggcca gcggggggag ccggcggcgt cagcagggcg gtgaggttcg gtggattcgg
     1441 cgtggccgac ttgatctcgg cgtcgaacgt ctgcaggccg gcttgcttct ggacgatgcg
     1501 cgtcggcacc tgcacgccgt tcatgttctg gtagttcgag tactcgaact cgaccaggag
     1561 gtcgccgacg accgcgtggt ccacgcgcgt ttctaccttc gtcacgaggt tctgcccgct
     1621 gatgtagccc gtcacgctgt acgtctgccc ggacggtgac ttcaggttgg gtggagtgaa
     1681 cgagacgagc tgctggccac cctgctgccg aacggtggcg ttgttggccg ccgcgccttt
     1741 cagaaagccc cagggagtcg tccagatgtt cagcgcctga ttccagttgt tggaggcctg
     1801 cgcgccggtg acgttctggt tgaacacgcc cggcatgggc ggtgcgacgc ctggcaccgt
     1861 gggcggctgc gtcgggccgg tcgcccggga gacgagcgcc gcggggtcgc ctggcggcgc
     1921 gaagttgatc gtacgggtgt attcggtgat ccgcgtcagg ttcacaggtc ccatgggatc
     1981 gccgatgctc ttgctctgcc cgaaggcgcc gtttcgcgct gttcccgagt aagtgatgga
     2041 agtgagcgtg tccacgccca tcgccttgga tgcggcggcg acgaccgagc cggcgttctg
     2101 cgcggccgtg cccgccgtga gcagggcgac gagcgcggca accgtgaacg ttctgagcag
     2161 catacgtgcc tccagacctc agtgaaagtc agtgtgcgga tcttatatac gcggggaagc
     2221 ggccggcgtt gaccgcgctt cctgcggaac ctcgacaaga ggcgccgccg ccgcccccgt
     2281 ccatacggcc agcggtgctc gaaggcggcc aagtaactcc ttcgtttcag gggggttgga
     2341 ccctcacggc ccgcccggcc gccgcgcatc gcccacgcat ggacacggga gcgatttcgg
     2401 cgtatatggg tgcacggcta ccagttgtcg ataccagcta ccaagctgat tgagaggtgt
     2461 ctgatgagtc acagaacgtt cctgacggcg gcggcgctcg tcctcggtct cgcggtcggc
     2521 ctcgttgcgg cgcaggacaa ggcacagacc aagacagcag tcggcgccgt cgccaagatt
     2581 gacggcacca acctgtcggt ggataccgga aagggagtga cactgcagtt cgtcacgagc
     2641 acgaccaccg gagtcaaggt cgcgacgggc agttcccagg cgcgcgaggc gaaggcggag
     2701 ggcaaggcgg gcgtcaagat caccgacgcg gtgcacgtgg gcgatcaggt ccaagtgagg
     2761 tacaccgacg tcggcggcaa gctgatggcc tctgaagtcg aggtgctgca gcgccggccg
     2821 gcgagtggac tccccgtcaa gtagagagcg cagggcagga ggggtcagat cccgatctgg
     2881 ccccgccgcg cctagagttg gccgctcctg gcgtcgagcg tggcgagcac acgtcggtac
     2941 gacgggtcag cgaagcgcgc gggatgaaac gccgccgcgt agtcgggcct cgggcggtcg
     3001 aggagatggc atgcaaggag ctctgcggcg gcttgtgagg ccatgatccc gaagccggag
     3061 agcgcgccta gtagaaagac gccctcaatc gacgtcggac cgatgagggg acgattgtcc
     3121 ggagccttgc agtagtagcc gccgtccacg acagccgctg cggcgcggct cacgtacgcg
     3181 ctcaggccgg gaaccatttc ggcaaggccg cggatcacaa cctctccgta gctgccgtcg
     3241 aacaccgggg ggaacctcgg cgtctcgatg cgcgtgtcgt aggtccagat ccccagaacg
     3301 gatcgcgcgc cgcgcggacg caaatgcacg cccggcggaa acgtcccgcg cgctccgaga
     3361 tcgacggggt cgttccagat catcagcgga gcgtcccgcg gaatcaggcc gtcctcgtct
     3421 tcgaatgaga ttttgccgtg cagctcgttg aggatcggca cgccgagccc gagtcgctcg
     3481 acccactccg gcaacagagg gccagccgcg aacaccagcg cgcgcgcgtc gattcgcgct
     3541 ccggacgcga gctccacggc aacgaggcgc tggtcggacg tgacgaggcg agtcacttcg
     3601 tcgcgcacga agtcgaggcc gcccgcgcgt gcacgctcga gcagatacgc tcccagcttc
     3661 aacgcgttca tgtagcccgc gcggcgcacg tggagcatcg cccgcgcacg ctcgttgacg
     3721 aacggaaaac gcgcatggat cgcgtcttcg ccctcgagga actccatcgc cgggccctcg
     3781 tgcgcgcgaa gccgctcgga ttccgccaga gtggctgtga ggaacacgta gccgcgtcga
     3841 ttgagctcga acgcccgacc gctgtcccga tcgagcgcct cgagcaggtc gatgctgcgg
     3901 ttcatgaacc gcgccatcgt gtcgtcgggg cccggccagt agttgcggta ggcttccgtg
     3961 cccttgctgc tcgtcagcga gagcggctcg cgcgggtcta cgagcgccac gcgccggacg
     4021 ccatcgcgca ccagcaactg gtgcgcggcg gcgacaccgg cgataccggc gcctgcgatg
     4081 acgacgtctg cggctcgcgg agcggacgcg gttctactcc agcacgattg gaagcacggc
     4141 ccgggtgcct tcccgctcga tgatcaggct gtaggtgccg ggagtccaga gtcctgtcgg
     4201 cgactcgaag aacagatcgc ccgtgatgac atggtcctta tcgctcacga agttcgtgag
     4261 caccggcccg ctctcgagcg cgaagagcct catcgactcg atccttctct tctcgaggaa
     4321 gtacgacagc acgtcgtgcc tgaggacgta ggcgccctgc accacggggt tgacccggct
     4381 gacgtcgctc gcaaaccgct gctggctcga cagcgtgatt acgcgctcac cggacggcgt
     4441 cacgagcgtg atgttctcgc gatcgatgac gaggttgcgc gtcgtggaca tcgccgactg
     4501 aatcagcagc caccgcgtat tgtggttcct ctgcgagtag tagtacgcgg ccacgacctg
     4561 gatttcagga tcccggtact cgatcgccgc ccggccccac cgcttcgttt tggggaagaa
     4621 gttgtcttcc tgcgcgcgca cggcagccgc gcttgcaagc gccaccatcg ccgccagcgc
     4681 cagcatcacc gaccggtgtt tgaaagagtc ctggctgttc atgggtctca cctcatccgg
     4741 accttcgagt tgcagaagtc gtgccgaccc atacaggcgc gttggcacat gcgccgtgcg
     4801 ttccctcgag caggcgcccg aagaaaggga caaccaagtc tacttcggcg tgagggcggg
     4861 cttcgcgaga ttgtcctcga accgctggtt ctcacgaacc gagcagatct gctggtagcg
     4921 cagaaagccg gtcttcgaca gcgtgaaata ccgctgtgcg ttccagggct gcgtatacgc
     4981 cccggggtcg tcgatcacca tcttcagctc gagtgag
//
LOCUS       DBNGSH010000366         1519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2408532, whole genome shotgun sequence.
ACCESSION   DBNGSH010000366 DBNGSH010000000
VERSION     DBNGSH010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2408532"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /gene="era"
                     /locus_tag="AC16CY_08600"
     CDS             <1..691
                     /gene="era"
                     /locus_tag="AC16CY_08600"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR3353886.1"
                     /translation="MNVRMVDVALEAIREVDVLALVVDSSVKPGPGDRYLLDLVKGAG
                     KPVLLVLSKVDLVAKPSLLPLIDEYRRAHPFAEIVPVSSADGTNVETLERLFLDYLPE
                     GEPLYPPEYLTDQTERFFVAEIVREQVLRLTHDELPFSTAVVVDRFDESERDLLKLYC
                     TILVERESQKAIVVGKGGSMIKQIGTAARLGLEAHFEQRVFLDLHVKVKSEWRDDERM
                     LDEISTQNRGG"
     gene            complement(696..>1519)
                     /locus_tag="AC16CY_08605"
     CDS             complement(696..>1519)
                     /locus_tag="AC16CY_08605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019643.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3353887.1"
                     /translation="DRAELFWALRGGGGNFGVVSSFEFTLHDIGPTIAGGLVAWPIAA
                     ARDVLRFFRDYTASIGDDVFTVCALLTGPDGATKLAAIAAAYLGPAAGTEAALAPVKS
                     FGSPVMDAMGPISYVALNGLLDGAFPKGALNYWKAPFLDRMEDRAIDAAVTAMASCPS
                     PMSQILFEHFHGAATRVPVEATAYALRSPGYNVNILGEWLDAGQTDANIAWVRGTYAA
                     LQSLASGRRYANYIGADEMTSEGLAAVYGSNLPRLRELKRKYDPENVFRENLNIT"
BASE COUNT          252 a          497 c          518 g          252 t
ORIGIN      
        1 gatgaacgtc cgcatggtgg acgtcgcgct cgaggccatc cgcgaggttg acgtcctggc
       61 gctcgtcgtg gacagctcgg tgaagcctgg tccaggggat cgatacctgc tggacctggt
      121 gaagggcgcc ggcaagccgg tactgctcgt gctgagcaag gtggacctcg tcgcgaagcc
      181 ctcactgctc ccgctcatcg acgagtaccg gcgcgcgcac ccgttcgccg agatcgttcc
      241 ggtgtcgagc gcggacggta ccaacgtcga gacgctcgag cgcctgttcc tcgactacct
      301 gcccgagggt gagccgctct atccgcccga gtacctgacc gaccagacgg agcggttctt
      361 cgtggccgag atcgtccgcg agcaggtgct tcggctcacg cacgacgagc tgccgttttc
      421 gacggcggtc gtcgtcgatc gcttcgacga gagcgagcgg gaccttctca agctgtactg
      481 caccatcctc gtcgaacgcg agtctcagaa ggcgatcgtc gtcggcaagg gcggcagcat
      541 gatcaagcag atcgggacgg cggcacgctt ggggctcgag gcgcacttcg agcagcgtgt
      601 gttcctcgac ctgcacgtga aggtgaaatc ggagtggcga gacgacgagc ggatgctcga
      661 cgagatctcg acgcagaaca gaggcggata gctgtttacg tgatgttcag gttctcccgg
      721 aacacgttct cggggtcgta cttccgcttc agctcgcgca gcctgggcag gttcgacccg
      781 tagacggcgg cgaggccttc gctcgtcatc tcgtcggcgc cgatgtagtt cgcgtaccgg
      841 cggccgctcg cgagggactg cagggcggca taggtgccgc gcacccacgc gatgttcgcg
      901 tccgtctgcc cggcatcgag ccattcgccg aggatgttca cgttgtagcc gggactgcga
      961 agtgcgtacg ccgttgcctc caccgggacg cgtgtcgcgg cgccgtggaa gtgctcgaac
     1021 aggatctgcg acatcggcga ggggcacgac gccatcgcgg tcacggcggc gtcgatcgcg
     1081 cggtcctcca tgcgatccag gaacggcgcc ttccagtagt tcagcgcgcc cttcggaaag
     1141 gcgccatcga gcagaccgtt cagcgcgacg taggaaatcg gccccatcgc gtccatcacc
     1201 ggagatccga agctcttgac cggcgccagt gccgcctcgg tgccggcggc cggtcccagg
     1261 tacgcggccg cgatcgcggc gagcttcgtc gccccgtccg gacccgtcaa cagcgcgcac
     1321 accgtgaaca cgtcgtcgcc gatcgaggcc gtgtagtccc tgaagaacct gagcacgtcg
     1381 cgcgcggcgg ctatcggcca cgccacgagc cctcccgcga tggtggggcc gatgtcgtgc
     1441 agcgtgaact cgaacgacga gacgacgccg aagttgccgc cgccgccgcg aagggcccag
     1501 aagagttcgg cgcgatcgg
//
LOCUS       DBNGSH010000367         2530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4276599, whole genome shotgun sequence.
ACCESSION   DBNGSH010000367 DBNGSH010000000
VERSION     DBNGSH010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4276599"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="AC16CY_08610"
     CDS             <1..614
                     /locus_tag="AC16CY_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353888.1"
                     /translation="PPEQIAAASERPANASAPDAGAASRRAAARAPIAPANETPAPAP
                     EPPPPAPVAAPADRRVVRGDAPDPSLVFETRALVGTLKPREQSAQLVLGEGKITVIPQ
                     DDPSKPLCSFPYGRVISISVSRNRDPLWNSPQGPAVVARAGGTIRRLGITVSRDWIAL
                     RTSTEEQFVAVRFDEILLKRVLLALEERTGRTPVFVELPREER"
     gene            777..1595
                     /locus_tag="AC16CY_08615"
     CDS             777..1595
                     /locus_tag="AC16CY_08615"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3353889.1"
                     /translation="MQLRVGAGTDTGLVRELNEDAYMSRAEQGLFVVCDGMGGAPAGE
                     VASQMAIEAIIGRLDGHGEPRECESQSFLPHTQRLADAVRHSNEFIYGQAQKDPRQAG
                     MGTTIVGAWIREHIASVAHVGDSRAYLWHDERLEPLTRDHSLAEARIAAGLDGDNLPA
                     CHQNALVRVLGREPDVDVDVTEVPVQPGDYVLLCSDGLTRMVAETELAQAITELQEPQ
                     RICDHLIAAANRNGGADNITVVVVEVKGSWWRRLLEGWKRTVRRGDDVETYAAV"
     gene            1570..2355
                     /locus_tag="AC16CY_08620"
     CDS             1570..2355
                     /locus_tag="AC16CY_08620"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3353890.1"
                     /translation="MSKLTLQFEGIGLREYAVGSGLTIGRLPDNKVVIDNPAVSGHHA
                     RVFLEGSSVVIEDLNSTNGTFVYGRPVARHVLRHGDEVLIGKHHLAFDATGEVAPVSS
                     PALQGLGDTVYLDTKQHRALRAGIESAKADAMRAAASPRPAVKAPVASSASSSARRVG
                     VLRVISGRSEHVEYELERHTSLIGRSATAQVQLQGWFKPYVAVAIARSGDSYVATLLG
                     GKTQINNQPLAARHELKDGDILNVSGLTLEFRWKDVMAAESAA"
BASE COUNT          425 a          843 c          893 g          369 t
ORIGIN      
        1 gtcctccgga acagattgcc gccgccagcg agcgcccggc caacgcgtcc gctcccgacg
       61 ccggtgccgc atcacggcga gcggcagcgc gcgcgccgat cgcgcctgcg aacgagacgc
      121 ccgctcccgc gccggagccg ccgccccccg cgccggttgc cgcgccagcc gatcggcgcg
      181 tcgtgcgcgg cgacgccccg gatccgtcgc tcgtgttcga gacgcgagcg ctcgtcggaa
      241 cgctgaagcc gcgcgagcag agcgcgcagc tcgtgctcgg cgaaggaaag atcacagtca
      301 ttcctcagga cgatccatcc aagccgctgt gctccttccc gtacggacgc gtgatctcca
      361 tcagcgtgtc gcgcaaccgg gatccgctgt ggaactcgcc gcagggaccc gcggtcgtgg
      421 ctcgcgcggg cggcacgatc aggaggctcg gcatcaccgt gtcccgagac tggattgccc
      481 tgcgcacgag caccgaggaa cagttcgtcg ccgtgcgctt cgacgaaatc ctgctgaagc
      541 gcgtgctcct cgccctggag gagcgcacgg gccggacgcc ggtgttcgtc gagctgccca
      601 gggaagagag atagttccca gccgatcccg cttctgtaac gcgtacacga aaagcgccac
      661 gggatcgcac ggggcggccg gtttgcgggt accgtcgcct gcccgcatct ctctccgaat
      721 caaggacttc agcccccagg gcgatcccgg cgtacgcctt gtaatcgatc gggctgatgc
      781 agctgcgagt cggagcgggc accgacaccg gactggttcg cgagctcaac gaggacgcct
      841 acatgtcgcg cgccgagcag gggctgttcg tcgtgtgcga cgggatgggc ggcgcgccgg
      901 cgggggaagt cgcgagccag atggcgatcg aggccatcat cggccggttg gacggacacg
      961 gtgagccgcg cgagtgcgaa tcgcagtcgt ttctgccgca cacgcagcgt ctcgcggatg
     1021 ccgtgcggca ctcgaacgag ttcatctacg gccaggcgca gaaggatccg cgccaggcgg
     1081 ggatgggtac cacgatcgtc ggcgcgtgga tccgcgagca cattgccagc gtcgcgcacg
     1141 tcggcgacag ccgtgcgtat ctctggcacg acgagcggct cgagccgctc acgcgggatc
     1201 actcgctggc ggaagcgcgg atcgcggcgg ggctggacgg cgacaacctg ccggcttgcc
     1261 atcagaacgc gctcgtccgc gtgctcgggc gcgagcccga cgtggacgtg gacgtgacgg
     1321 aggtgccggt gcaaccgggc gattacgtcc tgctgtgcag cgacggcctg acccgcatgg
     1381 tcgcggagac ggagctggcg caggcgatca ccgagcttca ggagccgcag cggatctgcg
     1441 atcacctgat cgccgcggca aacaggaacg gcggcgcgga caacatcacg gtggtggtcg
     1501 tggaagtgaa gggcagctgg tggcgccggc tgctcgaagg atggaaacgc acagtcagac
     1561 gaggcgacga tgtcgaaact tacgctgcag tttgagggaa tcggtctcag ggaatacgcc
     1621 gtggggtcgg gcctgacgat cggccgcctg cccgacaaca aggtggtcat cgataaccct
     1681 gcggtgtcgg gtcatcacgc ccgcgtcttc ctcgaaggca gcagcgtggt gatcgaggac
     1741 ctgaacagca cgaacgggac gttcgtctac ggccggccag tggcccgcca cgtcctacgg
     1801 cacggcgacg aggtcctcat cggcaagcac catctcgcct tcgacgccac gggcgaggtc
     1861 gcgcctgtct cctcgccggc gctccagggc ctcggcgaca cggtctacct cgacacgaag
     1921 cagcaccggg ccttgcgggc cgggatcgag tcggcgaagg ccgatgcgat gagagccgcg
     1981 gcgagtccgc gtcccgcggt gaaggcgccg gtcgcctcct ccgcctcttc ctccgctcgc
     2041 cgcgtcggcg tcctgcgcgt gatatccggg cgctcggagc acgtggaata cgagctcgag
     2101 aggcacacct cgctcatcgg gcgctccgcg accgcgcagg tccagctgca gggatggttc
     2161 aagccgtacg tggcggtcgc gatcgcgcgc agcggcgatt cgtacgtcgc gacgttgctc
     2221 ggcggaaaga cccagatcaa caaccagccg ctggccgcgc gccatgagct caaggacggc
     2281 gacatcctca acgtcagcgg tctgaccctg gagttccgct ggaaggacgt gatggccgcg
     2341 gaatcggcgg cgtagcggta cgcgctcggg tgcgcgggac gatcgagtct cgcgcacaag
     2401 gaagcagtaa ggccccagga atcaggcccc aggccgcagg gctgggcaag gtctgaggcc
     2461 tgagacggcc ctgaggcccg aggcctgagc cctgattcct gaagcctgat ttcttggtgt
     2521 tcggatcgac
//
LOCUS       DBNGSH010000368         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2578708, whole genome shotgun sequence.
ACCESSION   DBNGSH010000368 DBNGSH010000000
VERSION     DBNGSH010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2578708"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="AC16CY_08625"
     CDS             <1..1349
                     /locus_tag="AC16CY_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3353891.1"
                     /translation="RITERASRELMAVPGVRNFGAHIGRAEVADEVVGPNFTELWISL
                     DPSVDYEATVGRIQSIVDGYPGLYRDLLTYLRERIKEVLTGASATIVVRVYGPDLEQL
                     QVQAQAVRSAIAGVEGVADLAVQAQVMVPKVDVRFDPDRAAAVGLTPGEVRRAAATLV
                     QGTKVGEVYESQQLFDVIVWGAPETRQNLDAIRSLQVPVGNGGLVPLSSVSHVAITAT
                     PNEITRENGSRRIDVTSNVRGRDLGAVARDIRTRIAGMSFPQAYYAEILGEYAERQAS
                     STRLRLLGVTAVVAIFLILLADFGSVRVATMVFLTLPFALVGAIVAAFVTSGALSLGS
                     LVGLVTVIGISARNGIMLVSHYRHLELNEGVPFGRELVLRGAEERLAPILMTALATGL
                     ALVPIVFGGTRAGYEIEHPLAVVILGGLFTSTLLNLFIVPALYLRYGRPALTEGGA"
     gene            complement(1603..>1847)
                     /locus_tag="AC16CY_08630"
     CDS             complement(1603..>1847)
                     /locus_tag="AC16CY_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353892.1"
                     /translation="LSFYYVPSTMNEFLSEVRPDHIWLPPHLPVVPALRAAGWVPVFT
                     GPRSVWLTRRGETRPEKSEQWFLPQAEIAAGCFPGP"
BASE COUNT          300 a          593 c          634 g          320 t
ORIGIN      
        1 ggcgcatcac cgagcgcgcg agccgggagc tgatggccgt tccgggcgtt cgaaacttcg
       61 gcgcgcacat cgggcgcgcg gaagtcgccg acgaagtggt cggccccaac ttcacggagc
      121 tctggatcag cctcgatccg tcagtggact acgaggccac cgtcggtcgc atccagtcga
      181 tcgtggacgg ctacccgggg ctgtaccgcg acctgctcac ctacctccgc gaacggatca
      241 aggaggtgct caccggcgcc agcgccacga tcgtcgtacg ggtctacggg ccggacctcg
      301 agcagcttca ggtgcaggcg caggccgtgc gatcggcgat cgcgggcgtg gagggcgtcg
      361 ccgacctggc cgtgcaggcg caggtgatgg tccccaaggt ggacgtgcga ttcgatcccg
      421 atcgcgccgc ggccgtcggg ctcacgcccg gcgaggtgcg gcgggcggcg gccacgctgg
      481 tgcagggcac gaaggtcggc gaggtctacg agagccagca gctcttcgac gtcatcgtgt
      541 ggggcgcgcc ggaaacccgt cagaacctcg atgcaattcg atccttgcag gtccctgtcg
      601 gcaacggagg gctcgtgccg ctgtcgagtg tctcgcacgt cgcgatcacc gccacgccga
      661 acgagatcac acgcgaaaac gggtcgcgcc gcatcgacgt cacgtcgaac gtccgcggac
      721 gggacctggg cgccgtggca cgcgacatcc ggacgcggat tgcggggatg tcgttcccgc
      781 aggcctatta cgccgaaatc ctcggcgagt atgcggagcg gcaggcctcc tcgacccggc
      841 tgaggctgct cggcgtcacg gccgtggtgg cgatcttcct catcctgctc gccgatttcg
      901 gctcggtacg ggtggcgacg atggtcttcc tgacgctgcc gttcgcgctc gtcggcgcga
      961 tcgtcgcggc gttcgtcacg agcggagcac tctcgctcgg gtcgctggtc gggctcgtca
     1021 ccgtgatcgg catctcggcc cgcaacggca tcatgctggt gagccactac cggcatctcg
     1081 agctcaacga aggcgtgccc ttcgggcgcg agctcgtgct caggggcgcc gaggagcggc
     1141 tcgcgccgat cctcatgacg gcgctggcaa ccggcctcgc gctggtgccg atcgtcttcg
     1201 gcggcacgcg cgccggctac gagatcgagc acccgctcgc cgtcgtaatc ctgggcgggc
     1261 tcttcacgtc cacgctgctc aatctgttca tcgtgccggc gctgtacctc agatatggac
     1321 gccccgcgtt gacggagggc ggggcgtgag aaggaggggt gtcgttcgcg cttctcgctt
     1381 ctctgttcta ggttcgtgtt caatttcggg gttcgtgttc gggttctttg ttcgaggttt
     1441 gcttctcggt tcgcggcttg ctgagcatcg cggtaacggc acaatttgaa gggggtcggg
     1501 cggtgcgaac gccaaactgc gaacccggaa cctcgaatac gaacttgaaa ctgaacacga
     1561 accgagaagt gagaagcgag aagtgtgaac gactgttcct cttcaaggcc caggaaaaca
     1621 gccagcagcg atctcggctt gcggcagaaa ccattgctct gacttctccg gccgcgtctc
     1681 gccccgccgc gtcagccaga ccgatcgagg gcccgtgaac accggcaccc atccggccgc
     1741 gcgcagcgca gggacgaccg gcaggtgcgg aggcagccag atgtgatcgg ggcgcacctc
     1801 tgagagaaac tcgttcatgg tcgacggaac gtagtagaac gacaggt
//
LOCUS       DBNGSH010000369         3340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1730231, whole genome shotgun sequence.
ACCESSION   DBNGSH010000369 DBNGSH010000000
VERSION     DBNGSH010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1730231"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="AC16CY_08635"
     CDS             <1..592
                     /locus_tag="AC16CY_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3353893.1"
                     /translation="TGLSAYLERLALPNNERYYSFDCGLLHVVVLDSGASYAAPSTAM
                     TEWLENDLNASARDPEHPWIVVGFHFPPYSSGEYGSWEEGRAAWSPQFDRHGVALVLS
                     GHMHAYERSWPVDARGAVARRDYRDPGAPVYVVTGGAGRHGRLHQFPGPAPDWSAFRA
                     VAQHTLRVTVDKTRMSIMAIRPNGDVLDSFSITRGS"
     gene            838..2127
                     /locus_tag="AC16CY_08640"
     CDS             838..2127
                     /locus_tag="AC16CY_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HIR3353894.1"
                     /translation="MKTFRPYEPDQMLLLPPSLSDWVPEDHVARFVGDLVDSLDLSAI
                     EDTYDEERGYPPYHPCMMTKVLLYAYCTGTYSSRRMAAKLHDSVAYRFLAAGNQPDFR
                     TISEFRKRHEEALSGLFEQVLVICRESGLVKLGRVAVDGTKIKANASKHKAMSYGRMT
                     EKEAALKKEIKEMFRQAEAIDREEDRRYGPDRRGDELPEELARRETRLKKIREAKAAL
                     EAAAKKKAAAEGKDPEQSKPAAKDQHNFTDPESKIQKTRDGFIQGYNAQVAVDETFQV
                     IVAQHVTPMAADVNQLQPAVDSIAAALKVRPKAVLADAGYCSEENIRALETKGVEPFI
                     ATGRQKHGKTRPSPWGRAPKNMTLRQRMARKLLTARGQKAYSQRKVIVEPVFGQIKQA
                     RRFRQFLRRGVRRVRHEWSLVCTAHNILKLRTALMTI"
     gene            complement(2288..2617)
                     /locus_tag="AC16CY_08645"
     CDS             complement(2288..2617)
                     /locus_tag="AC16CY_08645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3353895.1"
                     /translation="MEKIPGWYQEQRKKHPQVIKAYETLGEACRKAGPLEGKALALVK
                     LGIAVGARLEGAVHSHTRRSVEAGATIDECRHVVLLATTTIGFPAMMAALSWVEDVAR
                     EDAGTSR"
     gene            2762..>3340
                     /locus_tag="AC16CY_08650"
     CDS             2762..>3340
                     /locus_tag="AC16CY_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353896.1"
                     /translation="MVRSAALALLLLGATVPPGAAQTPPPEIHLNVTQGPGPLDVTLQ
                     WTGGQGPFEVLRSAAPASVCAPESLLGTTDVALWFDTPPSGTFFYQVRSPFALEPPET
                     CNGIDDDCDGTIDDDATGCNAGACQACLDGACQSICGACDNCVAGACQTRCGACEVCA
                     GGICTSCSASACQTCAGGACVSTCDDTQCLACA"
BASE COUNT          577 a         1083 c         1190 g          490 t
ORIGIN      
        1 caccggtctg agcgcctatc tcgagcggct ggccctgccg aacaacgagc gttattacag
       61 tttcgattgc gggctgctgc acgtcgtcgt gctcgacagc ggcgcgagct acgcggcgcc
      121 ttcgacggcc atgacggagt ggctggaaaa cgatctgaac gccagcgccc gggatcccga
      181 gcatccctgg atcgtggtgg gattccactt tccgccctat tcctcgggag agtacggcag
      241 ctgggaggag gggcgcgcgg cctggtcgcc tcagttcgac cggcacggag tggcgctggt
      301 cctcagcggg cacatgcatg cctacgaaag gtcctggccc gtggatgcca ggggcgctgt
      361 cgcccggcgc gattaccggg atcccggggc cccggtctac gtcgtcaccg gcggcgccgg
      421 ccggcacgga cgtctgcacc agttcccggg gccggcaccc gactggtccg ccttccgggc
      481 cgtggcccag cacacactgc gcgtcactgt cgacaagaca aggatgagca tcatggcgat
      541 ccgcccgaac ggcgacgtgc tggactcctt ctccatcacc cggggctcct aggagcctgt
      601 ccgagtcatg gcatgggccg cgcgcgtggg gcttgcgcgt cccaggcgag gcgcgaggag
      661 gaggcgatgt ggtggcatcg gtgacgacga gcaacgaagc ctggggccgc aacccccatg
      721 cgccctgcgg gttcgccgcg aacgcgaccc atgcgatgac tcggacaggc tcctaggagc
      781 ctgtccgagt aatcatcttg ccattgacag tgagcggccg atgtggagac tggccgcatg
      841 aagacgttcc gaccgtacga accggatcag atgctgttgc tgccgccgtc gctgtcggac
      901 tgggtgccgg aagatcacgt ggcgcggttc gtgggcgact tggtcgacag cctggacctg
      961 tcggcgatcg aagacaccta cgacgaggag cggggctacc cgccgtacca cccgtgcatg
     1021 atgacgaagg tgctgttgta cgcctactgc acggggacct actcgtcgcg gcggatggcg
     1081 gcgaagcttc acgacagcgt ggcgtaccgg ttcctggcag cggggaacca gccggacttc
     1141 cggacgatca gcgagttccg caagcggcac gaggaggcgc tgtcggggct gttcgagcag
     1201 gtgctggtga tctgccggga gtcggggctg gtgaagctgg gccgggtggc ggtggacggg
     1261 acgaagatca aggccaacgc gtcgaagcac aaggcgatga gctacggccg gatgacggag
     1321 aaggaggcgg ctctcaagaa ggagatcaag gagatgttcc gccaggcgga ggccatcgac
     1381 cgggaggagg accgccggta cgggccggac cgccgcggcg acgagttgcc ggaggagctg
     1441 gcacggcgcg agacacggct gaagaagatc cgcgaagcga aggcggccct ggaagcggcg
     1501 gcgaagaaga aggctgcggc ggaaggcaag gaccctgagc agagcaagcc ggccgccaag
     1561 gaccagcaca acttcaccga cccggagtcg aagatccaga agacccggga cgggttcatc
     1621 cagggataca acgcgcaggt ggcagtggac gagacattcc aggtgatcgt ggcgcagcac
     1681 gtgacgccga tggcggcgga cgtgaaccag ttgcagccgg cggtggactc gatcgcggcg
     1741 gcgttgaaag tccgaccgaa ggcggtgctg gcggacgcgg ggtactgttc ggaggaaaac
     1801 atccgggccc tggagacgaa aggcgtcgag ccgttcatcg cgaccggtcg gcaaaagcac
     1861 ggcaagacgc ggccgtcgcc gtgggggaga gcgccgaaga atatgacgct acgtcagcgc
     1921 atggcgcgca agctcctgac ggcccgcggc cagaaggcct acagtcagcg caaggtcatc
     1981 gtggagccgg tgttcggaca gatcaagcag gcacgacggt tccggcagtt cctcaggcgc
     2041 ggggtgcggc gagtgcggca cgagtggtcg ttggtgtgca cggcccacaa catcctgaag
     2101 ctgcggactg cgttgatgac aatatgaaga ggacgaggag ggtcggcgcg ggcatcgaag
     2161 gccgccggcg gcatcggagc cggcaggaga ccgatctcga tcgcggtcag ccccatgtcg
     2221 tccacggcgc gtcatacgcc cgccctctca aagcgatctt tgttgggccc gttactcgga
     2281 cgggctccta gcgggacgtt ccggcgtcct ccctggcgac gtcctccacc cacgaaagcg
     2341 ccgccatcat cgccggaaac ccgatggtcg tagtggcgag caggacgacg tggcggcact
     2401 cgtcgatggt ggcgccggcc tcgaccgagc ggcgcgtgtg cgagtggacc gcgccctcca
     2461 gccgcgcccc gaccgcgatc cccagcttga cgagcgccag ggccttcccc tcgagcggcc
     2521 cggccttgcg gcaggcctcg ccgagagtct cgtaggcctt gatcacctgc gggtgcttct
     2581 tccgctgctc ctgataccag cccgggattt tctccacggc cacctccgtt cgccccgcgc
     2641 ccctggtgcg tcgggccggg tgatcataga tcgggcgcta caagaggcgt cgaatctctg
     2701 ctattctggc cgggaatcaa aagcgcagga acggacggag ggctccttga gaggaatccc
     2761 gatggtgcga tcggctgccc tggccctcct gctcctcggc gcgaccgtgc ctcccggcgc
     2821 agcccagact ccgccgccgg agatccacct gaacgtcacg cagggaccgg gcccgctcga
     2881 cgtgacgctc cagtggacgg gaggacaggg cccgttcgag gtcctccgat ccgcggcccc
     2941 ggcttcggtc tgcgccccgg aaagcctgct cggcacgacg gacgtcgccc tctggttcga
     3001 caccccgcct tcggggacgt tcttctatca ggtccgcagt ccgttcgcgc tcgagccccc
     3061 ggagacctgc aacggcatcg acgacgactg cgacggcacg atcgacgacg acgcgacggg
     3121 ctgcaacgcc ggcgcctgcc aggcgtgcct cgacggggcc tgccagagta tctgcggcgc
     3181 ctgcgacaac tgcgtcgccg gggcgtgcca gacccgctgc ggcgcctgcg aggtctgcgc
     3241 cggcgggatc tgcacctcgt gcagcgcgag cgcatgccag acctgcgccg gcggcgcctg
     3301 cgtcagcacc tgtgacgaca cccagtgcct ggcctgcgcc
//
LOCUS       DBNGSH010000370         3284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2579070, whole genome shotgun sequence.
ACCESSION   DBNGSH010000370 DBNGSH010000000
VERSION     DBNGSH010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2579070"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="AC16CY_08655"
     CDS             complement(<1..1141)
                     /locus_tag="AC16CY_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HIR3353897.1"
                     /translation="MYRLLSRTRAAVLGSVLALTALLSGGVPSLSANATNPFLWPYNQ
                     PTSVAFDQNQVYQAWQEWKVAQITSTNAGGNGRLRVMGGVGNSTTVSEGQGYGILLAS
                     IFDDQTTLDGLWLFTADHLNSKGLMDWHIGNPGQRLGTGAATDGDEDIAMGLVNACVK
                     VRQRAWPASPAGIDYCARATAVINAMYQWEVDKPGSQPAGGLSNNVGNELLPGDSWNL
                     PVEFPAGIINLSYFAPGFYTVFGKFTGNAAAWEAVNARNYDMVNLVQSKPGNCSGLVP
                     NWNQYGGDAQIVPWQPNNYSWWSYDAARFGWRIAVDKRWYGTSRARETMNEVGGFFSS
                     AGFDGIGEHTMNGQRVGSGPWPFFVANAGAAVWAADSPRAVNCGAA"
     gene            complement(1332..1688)
                     /locus_tag="AC16CY_08660"
     CDS             complement(1332..1688)
                     /locus_tag="AC16CY_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3353898.1"
                     /translation="MLTERRTAVTDHRVIRVIELIESRPSAELAVGSLAEAVRLSPSR
                     LRRLFVAHTGLTLSRYIKRAKLRRADDLVRTTFLSVKEIAAAVGLHDESHFVRDFKSL
                     YGSTPTERRRTALANR"
     gene            1921..>3284
                     /locus_tag="AC16CY_08665"
     CDS             1921..>3284
                     /locus_tag="AC16CY_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3353899.1"
                     /translation="MARVAMCQDVLMEYMGLMCISGVLKEAGHTVEVFFDDQRHEDRF
                     IAELRAFKPDVVLFSVLSPSLQWSLNVGRRVKSDIGALTVYGNVHVITSPNVIEDEGV
                     DVVCLGEGEYPMLELCDAIDRGADYSRIEGFWVKTADGIVRNTSRQDLVDVETLPFHD
                     RQMYDKYGFFRHSHYLRVMAGRGCPFHCSFCTNPVLMDHFGGKRYIRKRSPQSAIAEI
                     EHTIARHPHKVKFLFFIDEVLWVKNDWLREFFQLYKERIHLPFTGNFRFGAIMEEDIK
                     LLADAGATSLVFATESGDEAQRRGLLNKPVSNDEIIKIAAWLHKYGISFSASAFFGLP
                     GDTVEDHVKRLDFFRTINPTYLWTTFFQPYPGLALTKQEHVQLHLPKGKQFEVTLHHD
                     MYLDLPDRERLINLKKVYFLCAKFPSLTPFLVWLTKFHNPPLFTLLFGLHFTYYIFLF
                     ERVSL"
BASE COUNT          592 a         1072 c         1026 g          594 t
ORIGIN      
        1 ttgccgcgcc gcaattgacc gctcgcgggc tatcggcagc ccacacagcg gctccggcgt
       61 tggccacgaa gaagggccag ggaccgctgc ccacgcgctg cccgttcatc gtgtgctcgc
      121 cgatgccgtc aaagcccgcg ctgctgaaga agccgcctac ctcgttcatc gtctcgcggg
      181 cgcggctcgt cccgtaccag cgcttgtcaa cagcgatgcg ccacccgaat cgtgccgcgt
      241 cgtagctcca ccaggagtag ttgttcggct gccacggcac gatctgggcg tcccccccgt
      301 actggttcca gttcggcacc agtcccgaac agttgcccgg cttggactga accaggttga
      361 ccatgtcgta gttgcgggcg ttcaccgcct cccacgccgc ggcgttgccg gtgaactttc
      421 cgaagacggt gtagaagccg ggcgcgaagt acgaaaggtt gatgatgccc gccgggaatt
      481 cgacggggag gttccagctg tcgccaggca gcagttcgtt gcccacgttg ttcgacagcc
      541 cgccggccgg ctggcttcca ggcttgtcca cttcccactg atacatcgcg ttgatcacgg
      601 ctgtcgcgcg cgcgcagtag tcgatgccgg cggggctcgc cggccaggcg cgctgtcgga
      661 ctttgacgca tgcgttgacg agccccatgg cgatgtcttc gtcgccatcg gttgcggcgc
      721 cggtacccag gcgttgcccg ggattgccga tgtgccagtc catcagacct ttcgaattca
      781 ggtgatcggc ggtgaacagc cacaggccgt ccagcgtggt ctgatcgtcg aagatcgacg
      841 cgaggaggat gccgtagccc tgcccctcgg agacagtagt gctgttcccg acgccaccca
      901 tcacacggag gcggccgttt ccgcccgcgt tcgtgctggt gatctgggcc actttccatt
      961 cctgccacgc ctggtagacc tggttctgat cgaatgcgac gctggtcggc tggttgtacg
     1021 gccagaggaa cgggttggtc gcgtttgccg acaggctcgg tacgcctccg ctcaggagcg
     1081 ccgtcagcgc cagcaccgat cccagaacgg ccgcgcgtgt tctcgacaac agtctgtaca
     1141 tggctccgcc atctccgttg aggatccgga ttggcgagcg ttgggggcgc cctcacacga
     1201 gaggcgcaag accgatacgg gccgaccgcg tgctacaacc cagacaaaga aaaacgtgtg
     1261 cgtcctgaga cgccgacaca gaatcgtctc gcgcgtgcgc tgcgtcaagc gctatgacat
     1321 ggcgaatccg gctatctgtt ggcgagagcc gtccggcgac gctccgtcgg cgtggagccg
     1381 tagagggact tgaagtcgcg aacgaagtgg ctttcgtcgt gaaggcccac ggccgcggcg
     1441 atttccttga ccgacaggaa cgtggtgcgc accagatcgt cggcgcgacg cagcttcgcg
     1501 cgtttgatgt aacgcgagag cgtgagaccc gtatgcgcga cgaagagccg ccggaggcgg
     1561 gatggagaga ggcgcacggc ttcggcgagg ctcccgaccg cgagctccgc ggacgggcgg
     1621 ctctcgatca attcaatgac gcggatgaca cgatgatccg tgacggcggt acgcctctcg
     1681 gtaagcacca gctggcacca gccctctctg acccagcgta gcaccgaggt ctcggcgcgt
     1741 tagcgcggcc atggccaccg ccgagccttc gagctgaact gcatgctctg ccgaacgggc
     1801 gccggccgcg ctcgggaaag acggaaatcc gcggaaattt gcagaaccgc cctcgatgct
     1861 gtaatcatca agtcccgcat gccctcgccc gaaacgatca gtgtgagaga ctgactccgc
     1921 atggcccgcg tcgcgatgtg ccaggacgtg ctcatggaat acatgggcct gatgtgtatc
     1981 tccggcgtcc tcaaggaggc cggccacacg gtcgaggtct tctttgacga ccagcggcac
     2041 gaagaccgct tcatcgcgga gctccgggcg ttcaagcccg acgtggtgct cttttcggtg
     2101 ctcagcccca gcctgcagtg gtccttgaac gtcggacggc gcgtcaaatc cgacattggc
     2161 gcgctgaccg tgtacgggaa cgtgcacgtc atcacgtcgc ccaacgtgat cgaggacgaa
     2221 ggcgtggacg tcgtctgcct gggcgaaggc gaatacccga tgctggagct gtgcgacgcc
     2281 atcgaccgcg gggccgacta cagccgcatc gaaggcttct gggtcaagac ggccgacggg
     2341 atcgtccgca atacgagcag gcaggacctg gtggacgtcg agacgctgcc gtttcacgac
     2401 cgccagatgt acgacaagta cgggttcttc cggcacagcc actacctgcg cgtcatggcc
     2461 ggccgcggct gcccgttcca ctgctcgttc tgcaccaacc ccgtgctcat ggatcacttc
     2521 ggcggcaagc gctacatacg caagcgcagc ccccagagcg cgatagccga gatcgagcac
     2581 accatcgccc gccacccgca caaggtgaag ttcctgttct tcatcgacga agtgctctgg
     2641 gtgaagaacg actggctgcg cgagttcttc cagctctaca aagagcggat ccacctgccg
     2701 ttcacgggca acttccggtt cggcgcgatc atggaggagg acatcaagct gctggccgac
     2761 gcgggcgcca ccagcctggt attcgcgacc gaaagcggcg acgaggcgca gcgcaggggt
     2821 ctgctgaaca agccggtctc aaacgacgag atcatcaaga tcgccgcctg gctgcacaag
     2881 tacggcatct cgttctcggc cagtgcgttc ttcgggctac ccggggacac ggtcgaggac
     2941 cacgtcaaac gcctcgattt cttccggacg atcaacccca cgtacctgtg gacgaccttc
     3001 ttccagccct atccggggct ggcgctcacc aagcaggaac acgtgcagct gcacctgccg
     3061 aagggcaagc agttcgaggt gacgctccat cacgacatgt acctcgacct gcccgatcgc
     3121 gagcggttga tcaacctgaa gaaggtctac ttcctgtgcg cgaagttccc gtcgctcacg
     3181 cccttcctgg tgtggctgac gaagttccac aacccgccgc tcttcacgct cctgttcggg
     3241 ttgcacttca cgtattacat cttcctgttc gagcgggtgt cgct
//
LOCUS       DBNGSH010000371         2551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4108044, whole genome shotgun sequence.
ACCESSION   DBNGSH010000371 DBNGSH010000000
VERSION     DBNGSH010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4108044"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="AC16CY_08670"
     CDS             <1..959
                     /locus_tag="AC16CY_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIR3353900.1"
                     /translation="VFELRLKQISVTHLVGALVGGAVGLLAAKTLGAALYWANLSDGR
                     VVFLHSVILLAFPYLGLVIGARKGEWFEPENLVNMFRSAGPRKRYKVLDTSVIIDGRI
                     ADICETGFMEGTLVIPQFVLKELQLVADSSDSMKRNRGRRGLDILQKIQKMAGVEVTI
                     SDMDFPDVREVDLKLIELARALQGKIVTNDFNLNKVAQLRGVDVLNINELANSLKPVV
                     LPGELMKVFILKEGKEYNQGVAYLDDGTMVVVDNARKMIGRTIDVVVTSVLQTTAGKM
                     IFGRFLEASAVPPAQPGTASADSDSGRRPRPQSPPPAAAAGK"
     gene            956..1726
                     /locus_tag="AC16CY_08675"
     CDS             956..1726
                     /locus_tag="AC16CY_08675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3353901.1"
                     /translation="MNSPSYHWWRTVLFLIPAIGVYTIALGTLSLASSLFQRSGYFAH
                     WCARTWSRLILVTTGVRVSVAGLERLEAGKTYIFVSNHQSIYDIPILFWSLPFQLRII
                     AKQSLGHLPFLGWHLRRTGHMLVDRKHPKRSKIFGWAARLTSNGLSLIVFPEGTRSRD
                     GRVAPFKGGSFYLALQAGLPVVPLSVIGSRHVMLKGRLATYPGEVRLVVHDPIDTRPL
                     SGIDPKEFGERVRRIIAPDAESDLGAASPDVDALHARA"
     gene            1704..2336
                     /locus_tag="AC16CY_08680"
     CDS             1704..2336
                     /locus_tag="AC16CY_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B3/B4 domain-containing protein"
                     /protein_id="HIR3353902.1"
                     /translation="MPFTLAPDLAAIVRPGVLWLDEATVIERDARLNAPLAAAEAAVR
                     LSPPAEAASVRTMYKRVGLDPTKTRPSSEALLRRVRRGDPLPRINSMVDVCNWCSLEF
                     QLPYGLYDAAHIEGDVELRIGRAGESYPGIRKDIVHVEGRIALVDRLGPFGNPTSDSA
                     RTMVTKATTRAMLVVFAPCQIEATHVSKVLDATSRRMAEFTGCREAQRLL"
BASE COUNT          404 a          836 c          857 g          454 t
ORIGIN      
        1 tcgtcttcga gctccgcctc aagcagatct cggtcactca cctggttggc gcgctggtgg
       61 gcggggcggt cggattgctc gccgcgaaga cgctcggcgc cgccctctac tgggccaacc
      121 tgagcgatgg gcgcgtcgtc ttcctgcaca gcgtcatcct gctggccttc ccctatctcg
      181 gcctggtgat cggcgcccgg aagggtgagt ggttcgagcc ggagaacctc gtgaacatgt
      241 tccgctccgc ggggccgcgg aagcggtaca aggtgctcga tacgagcgtc atcatcgacg
      301 ggcgcatcgc cgacatctgc gagacgggct tcatggaggg cacgctcgtc atcccgcagt
      361 tcgtgctcaa ggagctgcag ctcgtggcgg actcctccga ttcgatgaag cgcaaccgcg
      421 gccggcgagg gctcgacatt ctccagaaga ttcagaagat ggccggcgtc gaggtgacca
      481 tctcggacat ggacttcccg gacgtacggg aggtagacct caagctcatc gaactggccc
      541 gggcgctgca ggggaagatc gtcaccaacg acttcaacct gaacaaggtg gctcagctcc
      601 gcggcgtgga cgtgctgaac atcaacgagc tggccaactc gctcaagccc gtcgtcctgc
      661 cgggcgagct catgaaggtg ttcatcctca aagagggcaa ggagtacaac cagggcgtcg
      721 cctatctcga cgatggcacg atggtcgtcg tggacaacgc acgtaagatg atcggaagga
      781 cgatcgacgt cgtggtgacg agcgtgctgc agacgacggc cggcaagatg atcttcggcc
      841 gctttctcga ggcgagcgcc gtaccgccgg cgcagcccgg cacggcgtcg gccgacagcg
      901 attctggccg gcggccgcgc ccgcagtcgc caccgccggc cgcggcggcc ggaaaatgaa
      961 ctcaccgtcg taccactggt ggcgaaccgt cctgtttctc atcccggcaa tcggggtgta
     1021 tacgatcgcg ctcggcacgc tctcgctggc gtcgagcctc ttccagcgca gcggctactt
     1081 cgcccactgg tgcgcgcgaa cgtggtcgcg gctgattctc gtgacgaccg gtgtgcgcgt
     1141 gagcgtggcc gggctggagc ggctcgaggc cgggaagacc tacatcttcg tctcgaacca
     1201 ccagagcatc tacgacatcc cgatcctgtt ctggtcgctg ccgtttcagc tgcggatcat
     1261 cgcgaagcag tcgctcggcc atctgccgtt tctcgggtgg cacctccggc ggaccgggca
     1321 catgctcgtg gaccgcaagc atcccaagcg ctcgaagatc ttcggctggg ccgcgcggct
     1381 gacgtcgaac ggactgtcgc tcatcgtgtt tcccgagggg acccggagcc gggatgggcg
     1441 cgtggccccc ttcaagggag gcagtttcta tctggcgctc caggcgggcc tgcctgtcgt
     1501 gccgctgtcg gtgatcggca gccggcacgt gatgctcaag ggacggctcg ccacctatcc
     1561 cggcgaggtg aggctcgtcg tgcacgatcc gatcgacacc aggcctctgt cgggcatcga
     1621 tccgaaggag tttggcgagc gtgtccgtcg catcattgct cccgacgccg agtccgacct
     1681 cggcgcggcg tcgcctgatg tcgatgccct tcacgctcgc gcctgatctc gcggcgatcg
     1741 tccgtcccgg cgtgctctgg ctggacgagg cgacggtgat cgagcgcgac gcccgcctca
     1801 atgcgccgct ggccgcggct gaagcggccg tgcgtctcag tccgcccgcc gaggccgcgt
     1861 cggtgcgaac catgtacaag cgcgtgggcc tggatccgac gaaaacgcgg ccgtcgtcgg
     1921 aagcgctgct gcgccgcgtc cggcgcggcg atccgctgcc tcggatcaac tcgatggtgg
     1981 acgtctgcaa ctggtgctcg ctggagttcc agcttccgta cgggctctac gacgctgcgc
     2041 acatcgaggg cgatgtcgag ctgcgcatcg gccgcgcggg cgagtcctat cccggcatcc
     2101 gcaaggacat cgtccacgtg gagggccgaa tcgcgctggt cgatcggctg gggccttttg
     2161 gaaacccgac gtccgactcg gcgcgcacga tggtgacgaa ggcgacgacc agggccatgc
     2221 tcgtggtctt cgctccctgc cagattgagg cgacgcatgt gtcgaaggtg ctcgacgcca
     2281 cgtcgcgccg catggcggag ttcaccgggt gccgcgaggc acagaggcta ctgtagactg
     2341 aggctcatgc ggagagccct cgtcgtcgtc ctcgtgatgt tcctgggcca cacaatcagc
     2401 gcgcagtggt tgaactctcc agcccccgga acgtctgggg cctgacgcct gggccctgag
     2461 gcctttcttg atgcacatct ctgccatcat cgccgctggt gggcgcggcc agcgcttcgg
     2521 aggcgggcac ctgaagcagc tgatgacgct c
//
LOCUS       DBNGSH010000372         2034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_711999, whole genome shotgun sequence.
ACCESSION   DBNGSH010000372 DBNGSH010000000
VERSION     DBNGSH010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_711999"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC16CY_08685"
     CDS             <1..246
                     /locus_tag="AC16CY_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3353903.1"
                     /translation="QCHGLRLKGDGPLAPKNPKPADLTDDKWDHGSSDGEIYAIIWNG
                     APAANSEMKPMKGTLAERDVWNVINYIRSVGPKTAKP"
     gene            254..883
                     /locus_tag="AC16CY_08690"
     CDS             254..883
                     /locus_tag="AC16CY_08690"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3353904.1"
                     /translation="MRTTHVRSWFVTTCFILASVLAGSVGCRVLDRNAPSEAPPLLPG
                     QTADVFTTARPTLAAAVNDFIGRHPVVVEQPFEFPHNIHAGKDIPCTEYCHEAVTQGP
                     VAGLPSVRTCITCHRTVATNRPRIRRITAMRDEGRDFAWRRVFAYPAPSHVRFNHAPH
                     IRASVECRTCHGDIGAQTVAQRNVEMTMGFCVTCHQERKAPLDCLTCHF"
     gene            900..>2034
                     /locus_tag="AC16CY_08695"
     CDS             900..>2034
                     /locus_tag="AC16CY_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3353905.1"
                     /translation="MDRRSFIKLTAISGTSAALASCGNPENEIIRFVPDEDIVPGIAA
                     MKPGVCTLCPSGCGLTVRVMSADADVVRKGQSGLVRINAAKKLEGSAGHPVNHGGLCA
                     RGQAAIQVTYHPDRITQPLKRAGDRGQGRYEAISWDDALAELVSRLDALAGSGNQKAL
                     AWLTRVRPGHRAALVSRFLASFGAPGPVSCELFGDDVLRRANALSFGREQLPTFDLPN
                     ARYVLGLGADFLGTWNSPTSQGYGYGLMRQGRPGIRGIFVQAESRMSQTGANADEWVP
                     VKPGTEGVLALGLAHVILAAKLRPASAGGRAAALIDGWSSGLAGYAPEQVEKVTGVAA
                     SRVDRLARDFAAISPAVAIIGGAPLAHTNGLFNALAVNALNALA"
BASE COUNT          326 a          697 c          682 g          329 t
ORIGIN      
        1 cagtgtcacg ggctgcggct gaagggcgac ggcccgctcg cgccgaagaa tccgaagccg
       61 gccgatctca ccgatgacaa gtgggatcac gggtcgagcg acggagagat ctacgccatc
      121 atctggaacg gcgcccccgc cgccaacagt gaaatgaagc cgatgaaagg cacgctggcg
      181 gagcgggacg tgtggaacgt catcaattac attcgtagcg tcggtcccaa gaccgccaag
      241 ccctgaccga ccggtgagga cgacacacgt ccgttcctgg ttcgtcacga cctgcttcat
      301 cctggcctcg gtgctcgccg gaagcgtggg ctgccgcgtg ctggatcgga acgcgccctc
      361 tgaggcaccg ccgcttctcc ccggtcagac agcggacgtc ttcacgacgg ctcgccccac
      421 gctggccgcc gcggtgaacg atttcatcgg ccggcacccc gtggtggtcg agcaaccctt
      481 cgagtttccg cacaacatcc acgccggcaa ggacatcccg tgcaccgagt actgccacga
      541 ggctgtgacg cagggtcccg tggcgggcct gccgagcgtg cggacgtgca tcacttgtca
      601 ccgcacggtg gcgacgaacc gcccgcgcat ccgccggatc acggcgatgc gcgatgaagg
      661 ccgggatttc gcctggcgcc gcgtgttcgc gtaccccgcg ccttcacatg tcaggttcaa
      721 tcacgctccc cacatccgcg ccagcgtcga atgccggacc tgccacggcg acatcggtgc
      781 gcagaccgtc gcccagcgga acgtcgagat gacgatgggg ttctgtgtga catgtcacca
      841 ggagcgcaag gcgccgctcg actgcctgac gtgccatttt tgagcgacgc gtaactcaca
      901 tggatcgccg ctctttcatc aagctcaccg ccatctcggg cacgagtgcg gcgctcgcca
      961 gctgcggcaa ccccgagaac gagatcattc gtttcgtccc cgacgaagac atcgtccccg
     1021 gcatcgcggc gatgaaaccc ggcgtgtgca cgctctgccc ctccgggtgt ggattgaccg
     1081 tccgcgtgat gagcgccgac gccgacgtcg tacggaaggg tcaatccggg ctcgtccgga
     1141 tcaacgccgc caagaagctc gagggaagcg ccgggcatcc ggtgaaccat ggcgggttgt
     1201 gcgcgcgcgg ccaggcggcg atccaggtga cctatcaccc cgatcgcatc acgcagccgc
     1261 tcaagcgtgc cggcgatcga gggcagggac gctacgaagc gatttcctgg gacgatgcgc
     1321 ttgccgagct cgtgtcgcgt ctcgacgcac tggccggctc cggcaatcag aaggcgttgg
     1381 cgtggctcac gcgcgtgcgg ccggggcatc gggcggcgct cgtcagccgg ttccttgcca
     1441 gcttcggggc tccgggcccc gtctcgtgcg agctgttcgg cgacgacgtg ctgcgccgcg
     1501 ccaatgcgct gagtttcggc cgcgagcagt tgcccacctt cgatctgccg aacgcgcgct
     1561 acgtcctcgg cctgggtgcg gacttcctcg gcacgtggaa ctcaccaacg tcgcagggat
     1621 atggctacgg gttgatgcgg cagggccgcc cgggcatcag agggattttc gtgcaggccg
     1681 aatcgcgcat gtcccagacc ggcgcgaacg ccgacgagtg ggtgccggtg aagccgggca
     1741 ctgagggcgt gctggctctc gggcttgccc acgtgatcct ggcggcgaag ctgcggcctg
     1801 cgagcgcggg cgggcgcgcc gccgcgctga tcgacggctg gtcgtcgggc ctcgccggct
     1861 acgcgccgga gcaggtggag aaggtcaccg gcgttgcggc ctcgcgcgtc gatcggctgg
     1921 cccgcgactt tgccgcgatc tctcccgctg tggcgatcat cggtggcgca ccactcgcgc
     1981 acacgaacgg ccttttcaac gcgctcgccg tcaacgcgct caatgcgctc gccg
//
LOCUS       DBNGSH010000373         5861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2070559, whole genome shotgun sequence.
ACCESSION   DBNGSH010000373 DBNGSH010000000
VERSION     DBNGSH010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2070559"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC16CY_08700"
     CDS             <1..417
                     /locus_tag="AC16CY_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3353906.1"
                     /translation="RVVDGNSVVRDLVVRGEAKVGLTDTDDVNVALEDGQPIAMVLPD
                     REGLGVPVMPNMVSLIAGAPHPVEGRLLIDYLLSADVERQLAQSEAVQIPLHRGVQGP
                     RNMPPIDTFKPMTLDYAAAAARVDDVTNRLAAILGL"
     gene            448..1980
                     /locus_tag="AC16CY_08705"
     CDS             448..1980
                     /locus_tag="AC16CY_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353907.1"
                     /translation="MWRSQRSRTIVIGVAFAAFVVCCVLPIAYLVATTLGAAGGPSAA
                     ILLDSRQRGLLSNTALLGVGAAVVATAIGAPLGVALARVPLRRKALLRLIAAAPVLLP
                     PYVVALAWIYLGVSEWTYSIPAAVLILGLLLYPISMLATEAAMRRVEGRLEEAALIVA
                     PPRRVLRRITLPLVAPSIVAAALIIFVLAISEFGVPGLLRVRVYTTEVFTAFAALYDF
                     SRAVVLAVPLLVVCAVVAVVAAVLLGERLVVTRRSSSGSPVVFGGWRRPAEVAMVGVI
                     ATAVVLPVLVLGREALAGRSLPAAVAGSAAAIVNSLSLAALGATLVVALALWLGLAQA
                     RARRAYRRAAQVVLVLLFAVPSTIVGIGLIGVWNRPGFFGALYGTDAMFLLAYLARFV
                     PVAALILAATAQTVSVSQEEAAAAGGAGWLRTIGGIVLPQMHVGIAAAWVVAFVLAFG
                     ELGVSILVAPPGEATLPIRVYTMIANAPSSQVAALALFQSLVIFTPLAAFAAAASLRK
                     VP"
     gene            1977..2678
                     /locus_tag="AC16CY_08710"
     CDS             1977..2678
                     /locus_tag="AC16CY_08710"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3353908.1"
                     /translation="MKAAPVALTNVSKRFSTHVALDDVSLAVTAGRAVVILGPSGCGK
                     TTLLRIIAGLEVPETGQVTLDGRTVSAAGRALVPPHERRVGFVFQDLALWPHLTVRQS
                     LDFVLGSTETAPGERGRRAQEALALVRIEQFSGRYPHELSGGEQQRAALARAIVGEPR
                     VLLLDEPFSSLDEELRADLRSELARLQRTLDLTTVYVTHDRDDAAALADSVVEMRAGR
                     IVAIHHDRSRTQDRA"
     gene            complement(2691..5306)
                     /locus_tag="AC16CY_08715"
     CDS             complement(2691..5306)
                     /locus_tag="AC16CY_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-alpha-glucoside-1,2-lyase-like
                     domain-containing protein"
                     /protein_id="HIR3353909.1"
                     /translation="MPAELPRLIPDPDFSQRVRVQDTEFSRDVLGRYLCNTLDEAVSS
                     TRVEAQPDVGRGERLDARKFDIVVLGGGTFGAAFAEHVWFRDTARQYRILVLEGGPFL
                     LPEHGQNLPMVGLGVADPSSVAEYNSRNEEERRNWRKEAWGLAWHSSTPFPGLAYAVG
                     GRSLYWGGWSPQLLPAEMPQSGVPLPWPSQVVSELRSLPGGDSYFRQASAEIGVNQTN
                     DFIFGTLHQALRERLFDKYAQVRHRVPLAELELHLDVDPHTPPGELARLKLEAPLAVQ
                     GRPPRAGFFPFNKFSAVPLLQKGVRAAINDANNDNVRRRLMVVPQCHVKHLQTAPGAA
                     GARRVTAIETNRGTVQLADVGSVVFALATIESTRVALESFRGVPNYSLIGRNLMAHLR
                     SNLDIRVPREALANLPAAEKELQTSALFVKGRKTIAGKVRHFHLQITASGLGRLGGNS
                     EAELWQKVPDGDHFEPFKHATDTHIVITIRAIGEMEPWDPDNPGAHNSLVRLDPDRDE
                     FQIERAFVQIQPTSHDLELWSAMDQASDDVAVLFGGGLPFEVFTPQGVKGVQPGDDLS
                     TLLFYTPKGHPDPTQRGRRDGLGTTHHETGPLWMGTDPSRSVTTPDARFHAVENAYVV
                     GPALFPSVGSPNPMLTGIALARRLGDHLGRPKPFEPEAGFAALFNGFDTSGWKMSTIK
                     NQPGRDNPGAFRVWNGTLESTAGTDLGLFWCTTPMPANFILRLDWLRWTHDSNSGVFL
                     RFPHPDSKGYNNTAYVADDFGFEVQIDELAQPDGRGIHRTGAIYRKDGREDNEVLTLK
                     PALPAGQWNAFEIRVEGGIFTVTLNSDVVCVFDNPYAGRGVPSTSTVPTFIGLQAYPR
                     PGWSVAYRKIRYRAL"
     gene            complement(5452..>5861)
                     /locus_tag="AC16CY_08720"
     CDS             complement(5452..>5861)
                     /locus_tag="AC16CY_08720"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR3353910.1"
                     /translation="DPVARNVRSLRAMTNDKRQSLGKTGEDLACAELERRGYAILARR
                     HRSRFGEIDIIASSHGAIVFVEVKARAGDEFGGSAAAVTAWKQRRIAQMAVDYLSRNG
                     LHDRPCRFDVVTVDVSGAQPHVVVFEHAFDSPI"
BASE COUNT          921 a         1901 c         1918 g         1121 t
ORIGIN      
        1 cgtgtggttg acggcaactc ggtcgtccgg gacctcgtcg tccgcggcga ggccaaggtc
       61 ggattgacgg ataccgatga tgtcaacgtc gcgttggagg atggacagcc catcgcgatg
      121 gtgctgcccg acagagaggg tctcggcgtc ccggtgatgc ccaacatggt ttcgctcatc
      181 gcgggggccc cgcatcccgt cgaaggccgc ctgttgatcg attacctctt gtcagccgac
      241 gtcgagcgcc agttggcgca atccgaagcc gttcagattc cgctgcatag aggagtgcag
      301 ggaccgagga acatgccgcc gatcgacacg ttcaagccga tgacgctcga ttacgcagcc
      361 gccgccgctc gagtcgacga cgtcacgaat cgcctcgcgg cgatcctcgg cttgtagctg
      421 ccgtcaaccg caaaagcggc ctttgtcatg tggcgaagtc aacgcagtcg cacgatcgtg
      481 atcggcgtcg cgttcgcggc gttcgtcgtc tgctgtgtgc tgccgattgc ctatctcgtc
      541 gcgacgacgc tcggagccgc tgggggcccg agcgccgcca tcctcctcga cagccggcag
      601 cggggcttgc tgtccaacac ggcgcttctg ggcgtgggag ccgccgtcgt tgcgacggcc
      661 atcggagcgc ctttgggcgt tgcgctcgcg cgtgttcccc tgcgtcgaaa ggctctgctg
      721 cgcctgatcg ccgccgcccc cgtcctgctg ccgccatacg tcgtggcgct ggcgtggatc
      781 tatcttgggg tgtcggaatg gacgtacagc atcccggctg ccgttctcat tctcggcctc
      841 ctcctgtacc cgatctcgat gctcgcgacc gaggcggcga tgcgtcgggt cgagggtcgc
      901 ctggaggagg cggccctgat cgtcgcgcca cctcgtcgtg tgttgaggcg catcacgctg
      961 ccgctcgtcg ccccgagcat cgtcgccgcg gcgctgatca tcttcgtgct tgcgatttcg
     1021 gagttcggcg tgcccgggtt gcttcgcgtg cgtgtgtata cgaccgaagt gttcaccgcc
     1081 ttcgctgccc tttacgactt cagccgcgcg gtcgtgctgg ccgtgccgct cctcgtggtg
     1141 tgcgcagtgg ttgcggttgt ggcggccgtg ttactgggcg agcgcctggt tgtcacccgc
     1201 cggtccagca gtgggtcgcc ggtcgtgttc ggtggatggc gcaggccggc ggaggttgcc
     1261 atggtgggcg tcattgcgac ggcggtcgtc cttcccgtac tcgtgctggg ccgcgaagcc
     1321 ctcgccggcc gctcattgcc tgccgccgtt gctggatccg cggcggcgat cgtgaacagc
     1381 ctgtcgttgg cggcactggg cgcgacattg gtcgtggcac tggcgctgtg gcttggtctt
     1441 gcacaggcgc gcgcgcgtcg tgcctaccgg cgtgccgcac aggtggtgct cgtcctgctg
     1501 ttcgccgttc cgagcaccat tgtcggcatc ggcctgatcg gcgtgtggaa ccggcccggc
     1561 ttctttggcg ctctgtatgg aacggatgcg atgtttctgt tggcgtacct ggcgcgcttc
     1621 gtgccagtgg ccgcactgat cctggcggca acggcgcaaa cagtatcggt gtcacaggaa
     1681 gaagcggccg ccgcaggtgg cgcgggatgg ctccggacga tcggcggtat cgttctaccg
     1741 caaatgcacg tcggcattgc ggccgcgtgg gtggtcgcct tcgtgctggc gttcggtgaa
     1801 ctgggtgtca gcatcctcgt cgcgccgccc ggcgaggcga cgctgcccat ccgcgtctac
     1861 acgatgatcg ccaacgcacc ctcctcgcag gttgccgcgc ttgcgctctt ccagtcgctc
     1921 gtcatcttta cgcccctggc ggcgtttgcc gcggccgcct cgctgcgcaa ggtcccgtga
     1981 aggcagcacc cgttgcgctg accaacgtct ccaagcgatt ctcgacacac gtcgcgctgg
     2041 acgacgtgtc ccttgccgtg acggccggaa gggcagtcgt cattcttggc ccgagcggct
     2101 gcggcaagac gacactgctc aggatcatcg ccgggctgga agtaccggaa acggggcagg
     2161 tcacgttgga cggaagaacg gtctcggcag cgggtcgcgc cctcgtgccg ccgcatgagc
     2221 gacgtgtcgg gttcgtgttc caggacttgg ccttgtggcc gcacctgacc gttcggcaga
     2281 gtctcgactt cgtccttgga tcgactgaga ctgcgcccgg cgaacgaggc cggcgagcac
     2341 aggaggccct ggcactcgtt cgcatcgagc agttctcggg gcggtacccc cacgagctct
     2401 ccggaggaga acagcagcgc gccgctcttg ctcgagccat cgttggcgaa cctcgtgtgt
     2461 tgctgctcga cgagcctttc tcgagtctcg atgaagagct tcgcgcagac cttcggtccg
     2521 aactcgcccg gcttcagcgc acgctcgatc tgaccacggt gtacgtcacg cacgatcggg
     2581 acgatgccgc cgcgctcgcc gactccgtcg tcgagatgcg cgccggacgg atcgtcgcca
     2641 ttcaccacga ccgaagtcga acgcaggacc gggcgtgaga cgcgatccct ctacagcgct
     2701 cgataccgaa tcttccggta cgccacgctc caccccggtc gcggatatgc ctgcagtccg
     2761 atgaacgtag gaacagtgct ggtcgacggg acacctcgtc ccgcgtatgg gttgtcgaac
     2821 acgcacacca cgtcgctgtt gagcgtgacg gtgaatatgc cgccctcgac gcggatttcg
     2881 aaagcattcc actggccggc cggcagcgcc ggcttcagcg tgagcacctc gttgtcctcg
     2941 cgtccgtcct tgcggtagat cgcaccagta cggtgaattc cccggccgtc cggctgcgcg
     3001 agctcgtcga tctgcacctc gaacccgaag tcgtcagcca cataggcggt gttgttgtac
     3061 cccttcgaat cgggatgagg gaaccggaga aagacgccgg agttcgagtc gtgcgtccag
     3121 cgcagccaat cgaggcgcag gatgaaattc gcgggcattg gcgtggtgca ccagaacagg
     3181 ccgaggtcgg tccccgccgt cgactccagc gtgccgttcc agacgcggaa cgccccggga
     3241 ttgtcacgac ccggctggtt cttgatcgtg ctcatcttcc agccactcgt atcaaacccg
     3301 ttgaagagcg ccgcgaagcc ggcctccggc tcgaatggct tcggacgtcc gaggtgatcg
     3361 cccagacggc gagccaacgc aatgccggtc agcatcgggt ttggcgatcc cacgcttgga
     3421 aatagtgccg gtccgacgac gtacgcattc tcgacggcgt ggaaacgcgc atcgggtgtg
     3481 gtgaccgaac gcgacgggtc ggtgcccatc cacaagggac ccgtctcgtg atgcgtcgtg
     3541 cccaacccgt cgcgacgccc ccgctgtgtc gggtccggat gccccttcgg cgtgtaaaac
     3601 aacagcgtcg acagatcgtc tccaggctgg acgcccttga cgccttgtgg agtgaacacc
     3661 tcgaagggca gccctccccc aaagagcacc gccacatcgt cagacgcctg gtccatggcg
     3721 ctccagagtt cgaggtcgtg gctggttggc tgaatctgca cgaacgcccg ctcaatctga
     3781 aactcatctc tgtcgggatc gaggcggacg agactgttgt gcgctccggg gttatcgggg
     3841 tcccaaggct ccatctcgcc aatcgcgcgg atcgtgatga cgatgtgcgt gtccgtcgcg
     3901 tgcttgaacg gctcgaagtg atcgccgtca ggaaccttct gccacagttc cgcttccgag
     3961 ttgccgccaa gccttccgag gcccgaggcc gtgatctgca gatgaaagtg acgaaccttg
     4021 cccgcaatgg tcttgcggcc cttgacgaaa agcgcggacg tctgcagctc cttctccgcc
     4081 gcgggaaggt tcgcgagcgc ttcgcgtgga acccggatgt cgaggttcga acggagatgc
     4141 gccatcagat tgcgtccgat caggctgtag ttcggaacgc ccctgaacga ttcgagcgca
     4201 acacgcgtgc tctcgatcgt ggcgagcgcg aacaccaccg atcccacgtc ggcgagctgc
     4261 accgtgccgc gattggtctc gatcgcggtg acgcgcctgg cgccggccgc acctggtgcg
     4321 gtctggaggt gcttcacgtg gcattgcggc acgaccatca gccgccgccg gacgttatcg
     4381 ttgttcgcgt cgttgatggc cgctcggacg cctttctgca ggagcggaac ggcgctgaac
     4441 ttgttgaacg gaaagaagcc ggcccgcggt ggccgaccct gcacggccag gggtgcctcc
     4501 agcttcaggc gcgcgagctc gcccggcggg gtgtgtggat ccacatccag gtgcaattcg
     4561 agctcggcga gagggacgcg atggcgaacc tgcgcgtact tgtcgaagag gcgttcgcga
     4621 agcgcctggt gcagcgtgcc gaagatgaag tcgttcgtct gattgacgcc tatctcggcg
     4681 ctcgcctggc ggaaatagct gtctccgcca ggcagcgaac ggagttccga gacaacctgc
     4741 gagggccacg gcagtggtac gcctgactga ggcatctcgg cgggcaacaa ttgaggagac
     4801 cacccgcccc agtacaagga cctcccgccg acggcgtacg ccaggccggg aaatggcgtc
     4861 gaagagtgcc acgccagtcc ccacgcctct tttcgccagt tacgtcgctc ctcttcgttg
     4921 cggctgttgt actccgccac ggacgagggg tccgccacgc cgaggcctac cataggcagg
     4981 ttctgcccat gttccgggag gaggaagggc ccgccttcga gcacgaggat tcgatactgt
     5041 cgtgccgtat cccggaacca cacgtgttcg gcgaacgccg ctccgaaggt gccgccgccc
     5101 agcacgacga tgtcgaactt cctggcatcg agtcgctcgc cgcggccgac gtcaggttgc
     5161 gcctccacac gggtgctgct gaccgcctcg tcgagtgtgt tgcagagata gcgtcccagg
     5221 acgtctcgcg agaattccgt gtcctgcacg cgcacgcgtt gcgagaagtc cggatcagga
     5281 atcagcctcg gtagctcagc tggcatgatt gcacctgcct tgtgactgcg ctgtggcgta
     5341 tgtgaagccg ccgcaccgtc ccttggcgtc agtgcgaggc cgtattgata aacgtgtgaa
     5401 ccgggaaatg cgaacgcggc ggtctacaga ggtcttcggt ctcaggttcc gtcagatggg
     5461 cgaatcgaag gcgtgctcga agaccacgac atgcggctgt gcgccgctga cgtcaacggt
     5521 gaccacatcg aagcggcagg gtcgatcgtg cagcccgttc ctcgacaggt agtccacggc
     5581 catctgagcg atgcggcgct gcttccaggc cgtcacggcg gctgcgctcc cgccgaactc
     5641 gtcgcctgcc ctggccttga cctccacgaa cacgattgcc ccgtgggacg aggcgatgat
     5701 gtcgatttca ccgaagcgcg atcgatgccg gcgcgcaaga atcgcgtacc cgcgacgctc
     5761 gagctcggca cacgccagat cttctccagt ctttccgaga gattgtcgtt tgtcgttggt
     5821 catggcccgg agcgatcgca cgttccgggc cacaggatct a
//
LOCUS       DBNGSH010000374         3240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3768702, whole genome shotgun sequence.
ACCESSION   DBNGSH010000374 DBNGSH010000000
VERSION     DBNGSH010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3768702"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="AC16CY_08725"
     CDS             <1..1110
                     /locus_tag="AC16CY_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3353911.1"
                     /translation="RIPMHMTGFPGIGKTEFTKSLEAGFTRAGVKCKVFTLVGSIREP
                     QDFGGFPVSTPDGVRLLPMAWARDARRLAEDGYLVIVFLDELTTVPPTTQAAMLRLLT
                     ENVCGELHLPDYEPGKGGVVYLCASNAVEWAAGGQEIQPPMANRLWHGEFPMDHETWC
                     DMMVSDFPAPSDLPVLPADYLYAHHHRQRSMIAAYVRAAGRDAWMAPPDDPARRSGAW
                     PSGRTWYLASRLLAAIEAVSPGNRVLQNAALAGLVGDQALPFLEYRETLNLPDPEDIL
                     RDPRSLVLPDRIDRAYAVCSSLVGAVLNNKTPDRWRAAMIALGVACRKNADIPTAAAR
                     TLCHKRNRPEGALTLPDECRPFYELMQLAKVLPRE"
     gene            1175..2545
                     /locus_tag="AC16CY_08730"
     CDS             1175..2545
                     /locus_tag="AC16CY_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3353912.1"
                     /translation="MLGRLAELIRGLDRKGAGYSRALQAARVRASYQRAYFAPALFNM
                     IPVRTDLIASMAVDSRWRLYYNDAWLAAHSVEENAAVLIHEVSHLLREHDARKQAAAV
                     QDEVLWNTAADCEINDDLAAEGLPLPGDPPQPGAYGLQTGENAETYYESMMRRREEGD
                     RGKGAGGRGTSPSLAAPSPPAPSPPAPCDCGSGAHGERRPWELPDDDGSPGGVPGVDS
                     VKAELVRRECAQKILDRTGDAGDVPFGWRRWARTVLSPKVDYMATIRHAVRKALRDST
                     LGRYDRTYRRPNRRQAAYGEFLMPSFHQPRPRPGFLIDTSASMEETQLARAVAELGGL
                     TRQLGYSTEVVVACCDAAVHDVKKVFNAKQLQLYGGGGTDIGAGLRWFTDRKGGPIDL
                     LVIVTDCVTPWPEEQPPFPVITIRVGDGAPPPWGDLGANRVITIEEPKVETTPMWNAR
                     SHRWQG"
     gene            2533..3117
                     /locus_tag="AC16CY_08735"
     CDS             2533..3117
                     /locus_tag="AC16CY_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353913.1"
                     /translation="MAGVTPPDMRWGRRPLIIPPHSARDWMQLAMWELWMGMLLCAGF
                     ALGVSLIATSPGHIAVVSDFTNCYLQPPVGSPCERIVYQGGALNAAFTALCGLTLVGA
                     AVWILWELWSAVEPKPVTDDFLRLLNDSFGRSWRNPLRWPWARILWAYGFASIGAMLT
                     VGAGLVVWTLVVATHPAKVPTPRVDTFQSFWPGR"
BASE COUNT          518 a         1088 c         1099 g          535 t
ORIGIN      
        1 cgcatcccga tgcacatgac cggctttccg ggcatcggca agaccgagtt caccaagagc
       61 ctcgaggcgg gattcacgcg cgccggcgtg aagtgcaagg tgttcacgct ggttggctcg
      121 attcgtgagc cgcaggactt cggcggcttt cccgtgtcaa cgcctgatgg tgtgcgtctg
      181 ctgccgatgg cgtgggcccg cgacgccagg cgcctggccg aggacggcta cctcgtgatc
      241 gtctttctcg acgagctgac caccgtgccg ccgacgacgc aggccgccat gctgcgtctg
      301 ctgacggaga acgtgtgcgg cgagctgcat ctgccggact acgagcctgg caagggcggt
      361 gtcgtttatc tgtgcgcaag caacgccgtt gagtgggcgg ccggcggaca ggaaatccag
      421 ccgccgatgg cgaaccggtt gtggcatggc gagttcccta tggaccacga gacctggtgc
      481 gacatgatgg tcagcgactt tccagcgccg agcgatctgc cggtgctgcc ggcggactat
      541 ctgtacgcgc atcatcatcg ccagcgctcg atgattgccg cctacgtgag agccgcggga
      601 cgcgacgcct ggatggcgcc gcccgacgat ccggcgcggc gaagcggcgc atggccgtcc
      661 ggccgcacgt ggtatctcgc gtcgcgactg ctcgccgcga tcgaagccgt gtcgccgggc
      721 aaccgcgtgc tccagaacgc ggcgctcgcc gggctcgtcg gcgatcaggc gctgcccttc
      781 ctcgagtatc gcgaaacgct gaacctgccg gatcccgagg acatcctgag ggacccgcgc
      841 agcctcgtcc tgccggaccg gatcgaccgc gcctacgccg tctgctcgtc gctcgtcgga
      901 gcggtcctga acaacaagac tccggaccgg tggcgggccg cgatgatcgc gctcggcgtc
      961 gcgtgccgga agaacgcgga cattccgacg gccgcggcgc gcacgctgtg ccacaagcgg
     1021 aaccggcccg agggagccct cacgctgccg gacgagtgcc ggccgttcta tgaactgatg
     1081 cagctggcca aggttctgcc acgggagtag agcgcacgct tccttgggcg ccgtagcgca
     1141 aagcgcgaag gcggcagcgt gcgcggacag gacgatgctc ggacgacttg cggaattgat
     1201 tcgtggcctg gatcggaagg gcgccggata ctcgcgcgcg ctgcaggccg cgcgcgtacg
     1261 cgcctcctat cagcgtgcct acttcgcccc ggcgctgttc aacatgatcc cggtcaggac
     1321 cgatctgatc gccagcatgg ccgtggactc gcgttggcgc ctctattaca acgacgcctg
     1381 gctggccgcg cactcggtgg aagagaacgc agccgtgctg attcacgagg tcagccatct
     1441 gctgcgcgaa cacgacgcgc gcaaacaggc cgccgcggtc caggacgaag tgctctggaa
     1501 caccgccgcc gactgcgaga tcaacgacga cctcgccgcc gagggactgc cgctgccggg
     1561 cgatcccccg cagcccggcg catacgggct gcagactggc gaaaatgccg aaacgtacta
     1621 cgagtcgatg atgagacgac gggaagaagg ggacaggggg aagggggcag ggggcagggg
     1681 gacttccccc tctctcgccg ccccctcgcc tcctgccccc tcgccccctg ctccgtgcga
     1741 ctgcggttcc ggcgcgcacg gcgaacgccg gccctgggag ctgcccgacg acgatgggag
     1801 ccccggtggg gtgccggggg tcgattccgt gaaggcggaa ctcgtgcgcc gcgaatgcgc
     1861 gcagaagatc ctggaccgaa ccggcgacgc cggcgacgtc cccttcggct ggcggcgatg
     1921 ggcgcgcacc gtgctgtcgc caaaggtgga ctacatggcc accatccgcc acgcggtgcg
     1981 caaggcgctg cgcgacagca cgctcggccg ctacgatcgc acctaccggc ggccgaatcg
     2041 ccgtcaggcc gcgtatggcg aattcctcat gccgtcgttt catcaaccgc ggccgaggcc
     2101 cgggttcctg atcgacacct cggcctcgat ggaggagacg cagctggcga gagccgtcgc
     2161 cgagcttggc ggcctgacac gtcagctcgg ctacagtacc gaggtcgtcg tcgcgtgctg
     2221 cgatgccgcg gtgcacgacg tgaagaaagt cttcaacgcc aaacaactgc agctctatgg
     2281 cgggggcggc accgacatcg gcgccggcct tcgctggttc accgatcgga agggcggccc
     2341 gatcgacctt ctggtcatcg tcaccgattg cgtgacaccg tggccggagg agcagccgcc
     2401 cttccccgtg atcaccatcc gcgtcggtga cggcgctccg ccgccgtggg gcgatctcgg
     2461 cgcaaaccgg gtgattacca tcgaagagcc gaaagtcgag acgaccccga tgtggaacgc
     2521 gaggtcacac agatggcagg ggtgacgccg cccgacatgc ggtggggccg ccgcccgctg
     2581 atcattccgc cccactcggc gcgcgactgg atgcaacttg cgatgtggga gctgtggatg
     2641 ggcatgctcc tctgcgcggg cttcgcgctc ggcgtctctc tcattgccac gtctccgggt
     2701 cacatcgctg tcgtgtcgga tttcacgaac tgttatctac agcctccagt cggctcgccc
     2761 tgcgaacgga tcgtctacca gggaggcgcg ctgaacgccg cgtttaccgc gctgtgtggt
     2821 ctgaccctcg ttggcgcagc tgtctggatt ctgtgggagt tgtggagcgc ggtcgagccg
     2881 aagcccgtca ccgacgattt ccttcgtctg ctcaacgact cgttcggacg cagctggcgc
     2941 aacccgctcc gatggccgtg ggcacggata ctgtgggcct acggctttgc ctcaatcggc
     3001 gcgatgctga cggtgggcgc tggcctcgtg gtctggacgc ttgtcgtggc gacgcatccc
     3061 gcgaaggtgc cgacgcccag ggtcgacacg tttcagagct tttggccggg tcgatagcga
     3121 acacgcgcgt ccatcacgca gatcgaaacc gcctcgcgta ctccccctat aatggctgcg
     3181 tgtctgccgg tgatggcgcg acccggtcgg ctgtgctgtc ccttgatacc tccgccgcca
//
LOCUS       DBNGSH010000375         2504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4277692, whole genome shotgun sequence.
ACCESSION   DBNGSH010000375 DBNGSH010000000
VERSION     DBNGSH010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4277692"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC16CY_08740"
     CDS             <1..482
                     /locus_tag="AC16CY_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3353914.1"
                     /translation="VTHAAVLDIVPTHYLYTHFKIEFVQRYFHWFNYLRPAPGPENDL
                     RAQNEAARARATSEVQIEYLRTASDPANIHAMCEDYRAGASIDLAHDEADMKKKIECP
                     LLVLWGQKGAMDGLYDVLGIWRERGTKAFGKSLPAGHNLQEDVPDLVFNEIQQLLKM"
     gene            498..1703
                     /locus_tag="AC16CY_08745"
     CDS             498..1703
                     /locus_tag="AC16CY_08745"
                     /inference="COORDINATES: protein motif:HMM:NF039593.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIR3353915.1"
                     /translation="MRALVTSVCVLILLGASSPAGQRNAPAPNLPSLPPISMTCAHHP
                     DVLELRPGTCPLCKMALVPVRLDSSWMCPVHTTIIETERGSCRLCRRQLVPVTVALTW
                     TCRGDAASHLEPGLCADGSPRIGQRTLRPHGNHNPRYGGQFFMAPDNWHHLEGVYPAA
                     RTFRLYLYDDYARPLSASAMREVEARVVTKETFDPATRKTTEVVSFPLRVSRNRAYLE
                     ARVDTRTLPAEMTAKVRFGRGQDEYRFDFTFDALTKAPAAPVSVPQPGQAAPVSPPTS
                     TSAPAAAPAQTSDAGLLPQVIPESMTEILEQLKARDGQVRDLIARGDFGAVWVPAFQA
                     RDLAIALEPHLAHLAARKREVGEPAISRVVRGAWLLDASGDVGNRQQVEAAYAAFQSA
                     VAEVVASFQ"
     gene            1700..>2504
                     /locus_tag="AC16CY_08750"
     CDS             1700..>2504
                     /locus_tag="AC16CY_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353916.1"
                     /translation="MTHRASRRTAIVLALAAAAIVVAVLPGGAHKPITSPYTFNEDVF
                     PILRDRCSRCHVTGGVAPMSLMTHADTVPWGESIRVELLAGHMPPWTVDAAPGRFRNV
                     SAMTAREMNVLLTWATGGTPLGDPEKAPPPSALNRDWPLGPPDLPLPFPREFTLPASV
                     QEDTVDVVIPAGTTERRWLRAVDLLPGSPSIVRAATVQLRAQTAPSAGAVADEPLLAM
                     WLPGDEPIMLDDAGFELPPGAELLVRVRYKKTWQFEREEMKDRSTIGLYF"
BASE COUNT          409 a          876 c          836 g          383 t
ORIGIN      
        1 cggtcacgca cgcggccgtg ctggacatcg tgccgacgca ctacctgtat acgcacttca
       61 agatcgagtt cgtccagcgc tacttccact ggttcaacta cctgcgtccg gcgcccggtc
      121 ccgagaacga cctgagagcg cagaacgagg cggcccgggc ccgggcgacc tccgaggtcc
      181 agatcgagta cctgcgaacg gcaagcgatc cggcgaacat ccacgcgatg tgcgaggact
      241 accgcgccgg cgcctccatc gacctggcgc acgacgaagc cgacatgaag aagaagatcg
      301 agtgcccgct gctcgtcctc tggggacaga agggcgcgat ggacgggctc tacgacgtgc
      361 tcgggatctg gcgtgagcgc ggcacgaaag cgttcggcaa gtcgctgccg gccggacaca
      421 atctgcagga ggatgttccg gatctggtct tcaacgagat ccagcaactc ttgaagatgt
      481 agcctgagac gtctgccatg cgcgcactgg tgacctccgt ctgcgtgctg atcctgctcg
      541 gggcatccag cccggccggg cagcggaatg cgcccgcgcc gaatctcccg agcctcccgc
      601 cgatctcgat gacgtgcgcg caccatccgg acgtcctcga gctccgcccg gggacgtgtc
      661 cgctctgcaa gatggcgctc gtccccgtac ggctcgattc gtcctggatg tgccccgttc
      721 acacgacgat catcgagacc gagcgcggtt cgtgccggct gtgccgcagg cagctcgtcc
      781 cggtcaccgt ggcgctgaca tggacgtgcc gcggcgacgc tgcaagtcat ctcgaacccg
      841 gcctgtgcgc cgacggatcg cccaggatcg gccagcggac gctgcgcccg cacggcaacc
      901 acaacccgag atacgggggc cagttcttca tggcgcccga caactggcat cacctcgagg
      961 gcgtctaccc ggcggcccgc acgttccggc tgtacctgta cgacgactac gcgcgaccgc
     1021 tgtcggcgtc ggcgatgagg gaggtggagg cgcgggtcgt gacgaaagag acgttcgacc
     1081 ccgccacgcg caagacgacc gaggtcgtct cctttccact gagggtgtcg cgcaaccggg
     1141 catacctcga ggcgcgcgtg gacaccagga cgctgcccgc cgagatgacc gcgaaggtcc
     1201 gcttcgggcg aggccaggac gagtaccgct tcgacttcac gtttgacgcg ctcaccaagg
     1261 cgcccgccgc gccggtatcg gtcccgcagc ccggccaggc tgcgcccgtc tcgccgccga
     1321 cgagcacatc agcgccggcg gccgctcccg cacagacgtc cgacgcggga cttctgccgc
     1381 aggtcattcc ggagtcgatg acggagatcc tcgagcagct caaggcgagg gacggtcaag
     1441 tgcgcgatct gatcgcgcgc ggagacttcg gcgccgtgtg ggtgcccgcg ttccaggcaa
     1501 gggacctcgc catcgcgctc gagccccacc tcgcccatct tgcggcgcgc aagcgagagg
     1561 tcggcgagcc ggccatcagc cgcgtcgtgc gcggggcatg gcttctcgat gcctcgggtg
     1621 acgtcggcaa ccggcagcag gtcgaagcgg cctacgcggc gttccagtcg gccgtggccg
     1681 aggtcgtcgc ctctttccaa tgacacaccg ggcaagccgc cgcaccgcga ttgtgctggc
     1741 gctcgccgcc gccgcgatcg tcgtcgccgt tctgccagga ggggcgcaca agccgatcac
     1801 gtcgccctac acgttcaacg aggacgtctt cccgatcctg cgcgaccggt gcagccggtg
     1861 ccacgtcacg ggcggcgtcg cgccgatgtc gctgatgacc catgccgaca cagtgccgtg
     1921 gggagagtcg attcgcgtgg agctgctcgc ggggcacatg cccccgtgga ccgtggacgc
     1981 cgcgccagga cggtttcgaa acgtcagtgc gatgacggct cgtgaaatga acgtcctgct
     2041 gacgtgggcc accggcggaa cgccgctcgg cgaccccgag aaggcgccac cgccgtccgc
     2101 tctcaacagg gactggccgc ttggccctcc cgatctgccg cttccgtttc cgcgcgagtt
     2161 cacgcttccg gcgagcgtgc aggaggacac ggttgacgtc gtgatcccgg cagggacgac
     2221 ggaacgccgc tggcttcgtg ccgtggacct gctgccggga tcgccttcaa tcgtccgggc
     2281 cgccaccgta cagctgcggg cacagactgc gccctcagcg ggcgcggtcg ccgacgagcc
     2341 gctgctcgcc atgtggctgc cgggcgacga gccgatcatg ctggacgacg ccggtttcga
     2401 gctgccgccc ggcgcggaac tgctcgttcg cgttcgctac aagaagacgt ggcaattcga
     2461 gcgcgaggaa atgaaggacc gcagcacgat cggcctctac ttcg
//
LOCUS       DBNGSH010000376         1653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1900491, whole genome shotgun sequence.
ACCESSION   DBNGSH010000376 DBNGSH010000000
VERSION     DBNGSH010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1900491"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..139)
                     /locus_tag="AC16CY_08755"
     tRNA            complement(43..139)
                     /locus_tag="AC16CY_08755"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Sec,seq:tca)
     gene            231..>1653
                     /gene="selB"
                     /locus_tag="AC16CY_08760"
     CDS             231..>1653
                     /gene="selB"
                     /locus_tag="AC16CY_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR3353917.1"
                     /translation="MQHVVVGTAGHIDHGKSSLVLALTGTDPDRLREEKERGITIELG
                     FAHTIENGIALSFVDVPGHERFVRNMLAGVAGMDLVMLHVAADESVMPQTLEHFEICR
                     LLHVPGGLVVITKSDLADPATLDVVRLEIQELVAGSFLDGAPVVAVSSRTREGIPELR
                     RTLASLASTARRRSSAGPPRMPIDRVFSMKGFGTVVTGTLLTGRLSQDDELVLEPSGR
                     VVKVRGIQVHGEAERAAVAGQRVAVNVAGAEVSDLTRGETLTVPGAVSVTRRVDVSFE
                     LLRTARPLAHGARVRFHHGAREVLGRVAISGGATVEPGTPGCARLHLEAPAVLVRGDR
                     FIMRAYSPLETIAGGTVLDPLPPRRGVRTAAGIARFARLAPPSSPLAAGTRVEGSGGD
                     ADAVMVMVEEAGVPGLPLAEIVGRAGIAPDARDPLIAELNRRGLAVSIGGVLVSAARL
                     ASVEDALVAAVGRYHSGHPLEEGI"
BASE COUNT          229 a          555 c          595 g          274 t
ORIGIN      
        1 ttcattggct tgccaaccga agctcgcgta gcgagcgaag gttggcggaa gcgcctggga
       61 gtcgaaccca gcctccctgc aaagcaggga gcagtccggt tttgaagacc gggagggcca
      121 ccgggcccca ttcgcttccg tcgtacgtag tgtccggctt tagccggacc ttctgaactc
      181 agtctatctc ctcgatcccg gtccgcctaa aggcggacac tactggccgg atgcagcacg
      241 tcgtcgtcgg aaccgccgga cacatcgatc acggcaagag ctcgctggtg ctcgcgttga
      301 cgggcaccga cccggatcgg ctgcgcgagg aaaaagagcg cggcatcacg atcgagctcg
      361 gcttcgcgca caccatcgaa aacggcatcg ccctctcgtt cgtggacgtg ccgggccacg
      421 agcgcttcgt gcgaaacatg ctcgccggag tggccggcat ggatctcgtg atgctccatg
      481 tggccgcgga cgagtcagtg atgccgcaga cgctcgagca cttcgagatc tgccggctcc
      541 ttcacgtccc cggggggctg gtcgttatca ccaagtccga tctcgcggac ccggccacgc
      601 tggacgtcgt gcggctggag atccaggagc tggttgccgg ctcgtttctc gacggcgcgc
      661 ccgtcgtcgc ggtctcgtcc cgcacacgcg agggcatccc ggaattgcgc cgcacgctgg
      721 cctctctggc ctcgacggcg cggcgacgat cctcggctgg gcctccgcga atgcccatcg
      781 accgtgtgtt ctcgatgaag ggttttggca ccgtggtgac ggggacgttg ctcaccggcc
      841 ggctctcgca ggacgacgag ctcgtgctcg agccgagcgg gcgcgtcgtc aaagtgcgcg
      901 gaatccaggt gcacggggaa gcggagcgcg ccgccgttgc cggccagcgg gtcgccgtca
      961 atgtggctgg cgccgaggtc tccgacctga cgcgcggcga gacgttgacc gtgcccggcg
     1021 cggtatcggt gacgcgccga gtggacgtgt cgttcgagct gctgcggacg gcgcggccgc
     1081 tcgctcacgg cgcgagggtc cggtttcacc atggggcccg cgaggtgctg ggccgggtgg
     1141 cgatctccgg tggcgcgacc gttgagcctg gcacccccgg gtgcgcgcgg ttgcacctcg
     1201 aagcgccggc cgtgctggtt cgcggcgacc gcttcatcat gcgtgcgtat tcgccactcg
     1261 agacgatcgc cggcggtacg gtgctcgacc cgctgccgcc ccggcgcggt gtccggacgg
     1321 cagccgggat cgcgcgattc gcccggctgg cgccaccctc gagcccgctc gcggcaggca
     1381 cgagagtcga gggaagcgga ggcgatgccg acgcggtgat ggtgatggtc gaggaggccg
     1441 gcgtgccggg tttgccgctt gccgagatcg tcggccgcgc gggcatcgcg ccggatgccc
     1501 gggatcctct gatcgcggag ctgaatcggc gcggccttgc cgtctcgatc ggcggcgtgc
     1561 tcgtctccgc cgcgcggctc gcatcggtcg aagacgcgct cgtcgccgcc gtcggcaggt
     1621 accactcggg ccacccgctc gaggagggga tcc
//
LOCUS       DBNGSH010000377         4222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3768757, whole genome shotgun sequence.
ACCESSION   DBNGSH010000377 DBNGSH010000000
VERSION     DBNGSH010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3768757"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..801
                     /locus_tag="AC16CY_08765"
     CDS             208..801
                     /locus_tag="AC16CY_08765"
                     /inference="COORDINATES: protein motif:HMM:NF019225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3353918.1"
                     /translation="MSGRARATVLAAFLLVVAGLPARSEASLIGAGPNNLTVIETITQ
                     QGLDWLYTYNITSADPSNVWHFLLYTSFATTNGTSTFPNVSPSGMSVVGLWPYDPRNV
                     NPFLTHVTNTWYAPFGGPNGLGTGGTATFSFVSSVFDASAKLFAYETAASGFAATTGR
                     VAAIGRTQSVPEPSSALLLIGGLACLAALRRRTTQTR"
     gene            934..2151
                     /locus_tag="AC16CY_08770"
     CDS             934..2151
                     /locus_tag="AC16CY_08770"
                     /inference="COORDINATES: protein motif:HMM:NF021508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase effector Tle1 domain-containing
                     protein"
                     /protein_id="HIR3353919.1"
                     /translation="MALYAFDGTWNEDDADVPDKATNVVRLRDAYDGPVEYREGVGTR
                     FGALGHVIGGLFGAGGRSRIEEMYDAAKANWARGDRVIDIIGFSRGAALALHFANILG
                     EKGLRLPDGRVEKPPIRFLGVWDIVGAFGLPLDFIINFQDINLGWTIDRVPATVQKCA
                     HGMALDERREAFDVTHLRKAPSVEEVWFRGVHADVGGGNGNVTRNNISFHWMLQQARS
                     AGLPLSPAFVELIGADVDPLASVSRNFDPMRDPRRTTKPEDMFHPSAVGRSLAVGETS
                     AFPVRAADQYNWSGVRLQKGASYRIDVAPDQRWIDGGINCDADGWTTESLPWFKEAVV
                     RHFEKERRLPGANWFALVAAVDDEDTHLFKIGNGGQFTSPRDGDLYAFANDMKGRYGN
                     NSGQIKATVRRLS"
     gene            complement(2159..2815)
                     /locus_tag="AC16CY_08775"
     CDS             complement(2159..2815)
                     /locus_tag="AC16CY_08775"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3353920.1"
                     /translation="MRVYTLVLSILVHAMAVAAVVIAPLFATTELPEPHRSSTLVVPR
                     PVTVADPDVPKERARAVEPRSEVEAPVAAAPIVAPTGISDPPPDLSIGGDTSPFPLVW
                     PGGNVDGGDPVPPPPARNEPPRHVGGVIRPPAKTVHVSPVYPAIARAAKVQGTVILEA
                     VISATGHVEDVRVLRSVQLLDDAAIEAVRQWRFTPTFLNGEPIPVVMTVTVTFTLTQQ
                     "
     gene            complement(2964..>4222)
                     /locus_tag="AC16CY_08780"
     CDS             complement(2964..>4222)
                     /locus_tag="AC16CY_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353921.1"
                     /translation="VVLDHPRRHLLRRLWQPIATMVGAVLFAAGIAASIVAVPASVLV
                     LAWLIGENVNIELTAGRTLTAREVFLAWAVATPLMFFGISRGLRLVRKGRTLVLFLRR
                     FGYDEAQSAVTFAVNSTIGRSWRVVTLDDAEIAPVGVPTGTRWLFGVVRMASALVTFA
                     VTLLFRIVPVAQLGLWIVVALDLVQSRIWERARNPQAWIAVLDPYLTIITTTFEGKLP
                     IDSIGPTLPGVFALLAVVLAGIVIGLGTALMAAPVAWAIGAVFLFLASFPAAAVFEAE
                     QGKTREIRDEGDLALAASAVTEKGRRLFGPQLVVLRVASSVWRQTVSRFASVSSVALI
                     DVSDPTENLVWEIEELTKRSRTRCVFICQRERAAQVVAGSLEGPYDDQVARLLELEEI
                     LAYTTDRRGRRRFARALRGTLMSKTT"
BASE COUNT          762 a         1369 c         1395 g          696 t
ORIGIN      
        1 cgagcgtggt acgcggctca cggcctgttc tgatttgcac gctttttcac tcacggcgtc
       61 ggacctgacg gctagccaga tggcgctcag gagccaataa attgcctgct ggattctcgc
      121 cttagtgcaa atgcccggtc tttgtgcgtg catggacctt gcacggtgtg gccatcgcgg
      181 tccattcccg cggaccaggg ggagacgatg agcggcagag cgagagcgac ggtccttgcg
      241 gcgttcctgt tggtcgtggc cgggctgccg gcgcggtcag aggcctcact gatcggggct
      301 ggaccgaaca atctcaccgt catcgaaacg atcactcagc aagggctcga ctggctctac
      361 acctacaaca tcacgagcgc cgacccttcc aacgtctggc atttcctgct gtacacgagc
      421 tttgcgacga ccaacggaac gagcaccttc cccaacgtgt cgccgagcgg gatgagcgtc
      481 gtgggtctgt ggccctacga cccgaggaat gtcaacccgt ttcttaccca cgtcaccaac
      541 acctggtacg cgccgtttgg cggaccgaac ggtctcggga ccggtggcac ggcaacgttc
      601 agctttgtgt cgagcgtctt cgacgcgagc gcgaagctgt ttgcctacga gacggcggcc
      661 tccggcttcg ccgccacgac gggcagggtg gcagcaatcg ggaggactca atccgtgccg
      721 gagccctcct cggccttgct gctgattggt ggtctcgcgt gtttggccgc ccttcgcaga
      781 aggacgacgc agacgcgcta ggaccgagcg gcctgaattc ttcctgaggg ccggccttcc
      841 tcgaggaacc gccggccctt gatccgtacg gccggttcaa ctgccactcc aatctatgat
      901 cgaggtcggc gcggaaggcc gaaaggagca cgcatggccc tgtacgcatt cgacggcacc
      961 tggaacgagg acgacgctga tgtgccggac aaagccacca acgtggtgcg cctcagggac
     1021 gcctacgacg ggcccgtcga gtacagagag ggcgttggca cgcgcttcgg tgctctcgga
     1081 cacgtcatcg gcggcctgtt cggcgccgga ggccggtcgc gcatcgagga gatgtacgac
     1141 gccgccaaag cgaactgggc acgcggcgat cgcgtcatcg acatcatcgg tttcagccga
     1201 ggggccgcac tcgccctgca ctttgccaac atcctcggag aaaaagggct ccgtcttcca
     1261 gacgggcgcg tcgagaagcc gcctatccgc tttctcggcg tgtgggacat cgtcggggcg
     1321 ttcggcctgc cgctcgactt catcatcaac ttccaggaca tcaacctcgg atggacgatc
     1381 gaccgggttc ccgcgacggt ccagaagtgc gcgcacggga tggcgctcga cgagcggcgc
     1441 gaggcgttcg acgtcacgca cctgcggaaa gcgccctcgg tcgaggaggt ctggttccgc
     1501 ggcgttcacg ccgacgttgg cggcggcaac ggcaacgtca cgcgcaacaa catcagtttt
     1561 cactggatgc tccagcaggc ccgctcggcc ggccttcccc tgtcgccggc gttcgtggag
     1621 ctcatcggcg cggacgtcga cccgttggcg agcgtctcca ggaacttcga tccgatgcgg
     1681 gacccgaggc gcacgaccaa gccggaagac atgtttcacc cgagcgcggt cggccggtcg
     1741 ctggctgtcg gcgagacgtc cgcgtttccc gtgcgcgccg ccgaccagta caactggtcg
     1801 ggcgtcaggc tgcaaaaagg cgcgtcgtat cggatcgacg tcgccccgga ccagcggtgg
     1861 atcgacggcg gcatcaactg cgacgccgac ggctggacca ccgagtcgct tccctggttc
     1921 aaggaggcgg tcgtcaggca cttcgagaag gagcgccgcc tccccggcgc caactggttt
     1981 gcgctcgtcg cggccgtaga cgacgaagac acgcatctct tcaagatcgg caacggggga
     2041 cagttcactt cgccgaggga cggcgacctc tacgcgtttg ccaacgacat gaagggccga
     2101 tacggcaaca acagcgggca gatcaaggcg acggtgagaa ggctgtcgta ggtcttgcct
     2161 actgctgggt aagtgtaaac gtcactgtca cggtcatcac gaccggaatc ggttccccgt
     2221 tcaggaacgt cggcgtgaac cgccattgac gaacggcttc aatcgcggcg tcgtcgagca
     2281 actgcaccga ccgcaggacc ctcacgtctt ccacatgtcc tgttgcggag atcaccgcct
     2341 cgaggatcac cgtcccctgg accttggcgg cgcgcgcgat cgccggatac acgggcgaga
     2401 cgtgtactgt tttggccggc ggtcgaatga cgccgccgac gtggcgcggc ggctcgttac
     2461 gagccggtgg cggcggcacg ggatcgccgc cgtccacatt accgcccggc cacacgagcg
     2521 ggaacggcga ggtgtcgccg ccgatcgaca ggtccggcgg cgggtcggaa atgcccgtgg
     2581 gcgcgacgat cggagccgcc gcgacgggcg cctcaacttc agagcgcggc tcgacggctc
     2641 tcgctcgttc tttcggcacg tcgggatccg caaccgtgac cggccgtggc accaccagcg
     2701 tgctcgagcg atgtggctcc ggcagttccg tcgtcgcaaa gagcggcgcg atcacgacgg
     2761 ccgcgacggc catcgcatgg acgagaatcg aaaggaccag tgtgtagacg cgcattgtgc
     2821 ctccctcggt tgacggcccg cgcgagcgcg ggaaactcga ccaggagagg ccttgccggc
     2881 gtagcctcgt tgcggagttg gacaccgggg gggagggatc gttccaagag tgcgaaggtg
     2941 cgagggtgcg aaggtgcgct ctgctacgtc gtctttgaca tcagcgtgcc gcgcagcgcg
     3001 cgcgcgaacc gcctgcgccc gcgccgatcc gtcgtatagg cgaggatctc ttcgagctcc
     3061 agcaggcgcg cgacctggtc atcgtacggc ccttcgagcg atccggcgac gacttgtgcc
     3121 gctctctcgc gctgacagat gaacacgcat ctggtccgcg atcgctttgt gagctcttcg
     3181 atttcccaga ccaggttctc tgttgggtcc gacacgtcga tcagcgccac cgaggatacc
     3241 gacgcaaagc ggctcaccgt ctgacgccat acgctcgagg caacgcgcag caccaccagc
     3301 tgcggaccga acagtctgcg ccccttctcg gtgaccgcgg atgcggcgag cgcgaggtct
     3361 ccttcgtcgc ggatctcgcg tgtcttccct tgctcggcct cgaaaacagc cgcggccggg
     3421 aacgacgcga ggaagaggaa cacggcgccg atcgcccagg caacaggagc cgccatcagc
     3481 gccgtgccca ggccgatgac gattcccgcg aggaccacgg cgagcagcgc aaaaactccg
     3541 ggaagagtgg ggccgatcga atcgatcggc agcttccctt cgaacgtcgt ggtgatgatc
     3601 gtgagatacg gatcgagcac agctatccac gcttgcgggt ttcgagcacg ttcccagatc
     3661 cgggattgca ccaggtccag cgccacgacg atccagagcc ccagttgcgc gacgggaacg
     3721 atgcggaaga gcagagtgac tgcgaaagtg acgagcgcag acgccatccg cacgactcca
     3781 aacagccacc gggtcccggt cggcacaccg accggcgcga tttcggcgtc gtccagcgtc
     3841 acgacgcgcc aggagcgtcc tatcgtgctg ttgacggcga aggtgacagc gctctgggct
     3901 tcgtcatagc caaagcggcg gaggaacagg acgagagttc ggcctttgcg cacgagcctc
     3961 agcccgcgtg agatgccgaa gaacatgagc ggtgtggcga ccgcccacgc gaggaagacc
     4021 tccctggccg taagcgtgcg gccggcggtg agctcgatgt tcacgttctc gccgatgagc
     4081 catgcaagta ccaggacgct cgctgggacc gcgacgatcg acgctgcgat cccggcggcg
     4141 aacagcacgg cgccgaccat cgtcgcgatg ggttgccaca ggcgtcgaag gaggtgtcgt
     4201 cgcgggtgat cgaggacgac cc
//
LOCUS       DBNGSH010000378         1936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3938706, whole genome shotgun sequence.
ACCESSION   DBNGSH010000378 DBNGSH010000000
VERSION     DBNGSH010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3938706"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="AC16CY_08785"
     CDS             complement(<1..839)
                     /locus_tag="AC16CY_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353922.1"
                     /translation="MTATRLARIALTSLVLAVLQAGLVHAQGGRASSAWTPPKTPWGH
                     PDIEGTYTNKDEANTPLERPDQYQGRSATEFSAADLAALAKERQALAAKIAGGIGGAE
                     TGAGPTHWYEHLQGNGSRAWLIIDPADGKLPALTPQGAKREAAWAALHNARNGEGRAD
                     SWLDRSLYDRCITRGLPGSMMPAIYGNAYEIVQTPEYVAIRYEMIHETRVIPLDNRPH
                     VAQGIRQYLGDPRGRWEGNTLVVETTNFTNQTHWAYNSRYNSEKYRLTERFTPTAPNT
                     LLW"
     gene            905..1771
                     /locus_tag="AC16CY_08790"
     CDS             905..1771
                     /locus_tag="AC16CY_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR3353923.1"
                     /translation="MPSPASRLRLLLGRPGLLVMPGCHDAMSARLIEEAGFELGFMSG
                     FGVSADRLGMPDTGLISYAELVDQGRNICRAVSIPLIGDGDTGFGSAQNIKRTVEGYA
                     SAGFACIMLEDQVAPKRCGHTEGKTVVGRDEALTRIHAAVDARDAGADILIMARTDAR
                     ACVSLDEAVTRCRMFRSIGADITFLEAPTGEEEMQRYCAEVDGPKMANLIEGGRTPIL
                     PPARLREIGYTIAVYPLTLLNASIVAMRTALRSLRNGETPAGIMDFEGLKEAVGFPEY
                     YAEEARYKTATG"
BASE COUNT          304 a          641 c          656 g          335 t
ORIGIN      
        1 tgccacagca gcgtgttcgg cgccgtcgga gtgaagcgct cggtgagccg gtacttctcg
       61 ctgttgtatc ggctgttgta ggcccagtgc gtctggttcg tgaagttggt cgtctccacg
      121 accagcgtat tgccttccca gcgtccgcgc ggatctccga ggtactggcg gatgccctgt
      181 gccacgtgcg gacggttgtc cagtggaatg acacgggtct catggatcat ttcgtagcga
      241 atcgcgacgt actcgggcgt ctggacgatc tcgtatgcgt tgccgtagat ggctggcatc
      301 atcgagccgg gcagcccgcg cgtgatgcag cggtcgtaga gacttcgatc gagccacgaa
      361 tcggcgcgcc cttctccgtt tcgcgcgttg tgcagcgccg cccacgccgc ctcccgcttg
      421 gcgccctgcg gcgtcagggc cggcagcttc ccgtcggccg gatcgatgat gagccaggcg
      481 cggctgccgt tgccctgcag gtgctcgtac cagtgcgtcg gtcctgcgcc cgtctcggcg
      541 ccgccgatgc cgccggcgat cttcgcggcc agggcctgtc gctctttcgc gagggccgca
      601 agatcggcgg cgctgaactc cgtcgcggat cggccctgat actgatcggg gcgctcgagc
      661 ggggtgttcg cctcgtcctt gttggtgtag gtcccctcga tgtcggggtg tccccacggc
      721 gtcttcggag gcgtccacgc cgacgacgcc cgtccgcctt gggcatggac cagcccagcc
      781 tgcagaaccg cgagtactag agacgtcagc gcgattcgcg ccaggcgagt tgctgtcatg
      841 tgcggcctcc cgatcggtca tatgttacgc gcacacagcg gcctgtgaga tacgattgcc
      901 ggccatgcca tccccggcct cgcggctgcg gttgctcctg ggccgcccgg gacttctcgt
      961 catgccgggc tgccacgatg cgatgtcggc gcgcctgatc gaggaggccg gcttcgagct
     1021 cggtttcatg agcgggtttg gcgtctcggc cgaccggctc gggatgccgg ataccggact
     1081 gatttcctac gccgagctcg tcgatcaggg gcggaacatc tgccgcgccg tgtcgatacc
     1141 gctcatcggc gacggcgata ccggattcgg gagcgcgcag aacatcaagc gcaccgtcga
     1201 ggggtacgcc agcgcgggtt ttgcctgcat catgctcgag gatcaggtcg cacccaaacg
     1261 atgcggacac acggagggaa agacggtcgt ggggcgagac gaggcgctga cgcgaatcca
     1321 cgcggcggtc gacgcgcgcg acgccggcgc ggacattctg atcatggcgc ggaccgacgc
     1381 gcgcgcgtgc gtgagcctcg acgaagccgt cacccgctgc cggatgttcc gctcgatcgg
     1441 cgccgacatc acgttcctcg aagcgccgac cggcgaggaa gagatgcagc ggtactgcgc
     1501 ggaggtggac ggtccgaaaa tggcgaacct gatcgagggt ggcaggacgc cgatcctccc
     1561 gcccgcgcgg ctccgggaga tcggctacac catcgccgtg tacccgctga ctctcctcaa
     1621 cgcgtcgata gtcgcgatgc gcacggccct gcgcagcctt cggaacggcg aaacgcccgc
     1681 cggtatcatg gacttcgagg gcctcaaaga agctgtcggc ttccctgagt actacgccga
     1741 ggaggcacgg tacaagactg caacaggata gaaaccacgg ttcgtcatcg cctcgatgct
     1801 ggcctcaccc gcatggccgc gatgaagacg ccaccgacgg cggcgaagga gagcaggccc
     1861 aggatcgtca cccacccacc gatcgccgcc gggcgcagga gccgccagag cgcggcgcca
     1921 aagtcgaatc cctgcc
//
LOCUS       DBNGSH010000379         5991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1731931, whole genome shotgun sequence.
ACCESSION   DBNGSH010000379 DBNGSH010000000
VERSION     DBNGSH010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1731931"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="AC16CY_08795"
     CDS             <1..440
                     /locus_tag="AC16CY_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025109.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein DrrA1-3 family
                     domain-containing protein"
                     /protein_id="HIR3353924.1"
                     /translation="RRGATILLSTHVMVHAEEICQQVVMIHQGRKVLDEPVAGLRRQY
                     DPHALLFEPLDPRADVTPLSRLPGVSRVEAADGTYRIALADGTDPAAFMPRVAAAVPA
                     ARVELSRVRLEDVFVRIVSGGLKLDEAARLRATLIPESDGASA"
     gene            437..1801
                     /locus_tag="AC16CY_08800"
     CDS             437..1801
                     /locus_tag="AC16CY_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3353925.1"
                     /translation="MKKVALIAGREFIAAVANKGFVIGLLLMPVVVAVLAFVGPRIMN
                     ASSTQISGEVAVIDPTGTVVAELKKTLDPAAIARRRTEATARALANAPAAVRGVVGTS
                     GTAIDTVLAGTPKLNIIEREPSADVQREKSWLTAPSTGARHLALVVVHPDAAEARAGS
                     DFGSYDVYVPPNLDERVENTLYEGFREAVLTARIRGRQLDRAQVETVMRVTRPTSVTV
                     TAENERRTNVGFNRSLPFIFGGLLVFGIMIGGQTLLTSLIEEKSSRVVEVLLSAVSPL
                     ELMAGKILGQMAVSMLVLALYLGAGLALLTSFAMIGLLDPMLIVYLLIFFVISYLVFA
                     AAFGAVGAAVNEMREAQSLMTPVMLALMSPWMLGPMIAREPNSTFAVAASFIPPVNTF
                     AMMTRLASSSPPPAWQVWATVAVGLASAFAVTWFAAKVFKVGLLMHGKAPSFSTLVRW
                     AREA"
     gene            1815..2522
                     /locus_tag="AC16CY_08805"
     CDS             1815..2522
                     /locus_tag="AC16CY_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR3353926.1"
                     /translation="MPIWVGTSGYNYPEWKGSFYPENLPAAKMLPYYAERFPTVEINY
                     TFYRAPNEKILEGWNKATPDRFKLTLKAPRRITHDARLRDCADRVRQFLETSANLGPK
                     LGALLFQLPPNLKKDLALFDLFLEAFPPRVCAAFEFRHESWLDAEVYERLRSRNLALC
                     VADSERLSTPVEITADYAYLRLRDEGYTTDDIARWASTIRERASGCKDVFVYFKHEEE
                     GKGPEFARTLIDHLGAP"
     gene            complement(2452..3486)
                     /locus_tag="AC16CY_08810"
     CDS             complement(2452..3486)
                     /locus_tag="AC16CY_08810"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HIR3353927.1"
                     /translation="MTFTASRFRPRFTNGHVQTLYAWARPRTFPRLPPPSERFFDVAP
                     DARVLAHCHWQDRAVEHPTLVLLHGLEGSSLVHYMGGMADKAWALGWNVVRLNQRNCG
                     GTEHLSRGLYHSGLTDDPRFVIRELVERDRIRAIAVAGYSLGGNLALKLAGDFGESAP
                     PELKAICAVSPTMDLAVCVKALERPANIPYQWNFVRRLKARMRRKAAIVPGLFPLEPL
                     RRIWTVRQFDEAYTAPHHGFRDAADYYFRASAMRVIDRISVPALIVTAEDDPFVPVEP
                     FAHHSVTGNPLVTTVVTRHGGHCAFLEHASGSYDGYWAEREVVRFVTAHRDGRSASAQ
                     TPAPSPLPRA"
     gene            3692..>5991
                     /locus_tag="AC16CY_08815"
     CDS             3692..>5991
                     /locus_tag="AC16CY_08815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR3353928.1"
                     /translation="MTRMATFVGALSIAASVGVGTLAQEPAPPPQDTPAQTPGQTPGP
                     PGRGGANQDPRPYEQVITKEAKSDTGVFTVHRIRDRIYYEIPKSELGKEFLWVSQIAR
                     TTLGAGWGGQAVSDRVVKWERLNNRVLLKGIAYDVVADPLSPIGRAVQAANNDTIIAS
                     FNVEAVGKDEAPVIEVTRLFTTDVPEFSARARLRGRGMDASRSFLDRASSFPQNIEVE
                     ATHTYTAGTDAPAGAAPVQPSAIRGGSGTVVMHFSMVKLPEKPMMPRLFDDRVGYFSI
                     QKYDYSLEEHRAPRRRYITRYRLEKKDPGAALSEPVKPIEYWIDPATPTKWIPFVKKG
                     IEAWQPAFEAAGFKNAIVARDAPSPAEDPDWSPEDARYSVIRWLPTTTENASGPHIHD
                     PRTGEILEADIQMHHNVMNLVRDWYFVQVGHLDPRAQKLPLPDALMGRLVEFVVAHEV
                     GHTLGFQHNMKASSTYPADKVRDPAWVKKMGHTPTIMDYSRFNYTAQPEDRIALEDLV
                     PGIGPYDKWATHWGYAPIAGAALPDDEKATLDRWSREQDSTPWLRFSTADAQGVDPGE
                     LTEAVGDADAIRASTLGMKNLQRVGAMLLTATTAKPGEPYDDLEELYGRLLGQWVLEM
                     NHVAALIGGFDTRQKHAGQDGVRFTIVPKQRQAAAVAFLNANAFTVPSFAVQPEILRR
                     IEPSGVLQRVQTAQLRVLTSVLTPARFARLIEQEALDGAAAYKATDFLRDLRRGIWRE
                     LGSSQVAIDAYRRGTQRLFLDAMAERL"
BASE COUNT          997 a         1991 c         2010 g          993 t
ORIGIN      
        1 atcgccgcgg cgccacgatt ctcctgtcca cccacgtcat ggtgcacgcc gaagagatct
       61 gccagcaggt ggtgatgatc catcaggggc gcaaggtgct cgacgagccc gtggcagggc
      121 tgcgccgtca gtacgatcct cacgcgctgc tgttcgagcc gctggacccg cgcgccgacg
      181 tgaccccgtt gtcgaggctc ccgggcgtca gtcgcgtgga ggcggccgac ggaacttacc
      241 ggatcgcgct ggctgacggt accgatccag ccgcgttcat gccgcgagtc gcggcagcgg
      301 tgccggcggc cagggtggag ttgtcgcgcg tccggctcga ggacgtgttc gtccggatcg
      361 tgtcgggtgg tctgaagttg gacgaggccg cccggctgcg cgcgaccctc attcccgagt
      421 cggatggagc ctcggcatga agaaagtcgc gctcattgct ggccgtgagt tcatcgccgc
      481 cgttgccaac aagggcttcg tcatcggatt gttgctgatg ccggtggtcg tcgccgtgct
      541 cgcgttcgtc ggaccccgca tcatgaacgc cagcagcacg cagatcagcg gggaagtggc
      601 cgtcatcgac cccactggca ccgtggtcgc cgagttgaag aagactctcg atccggccgc
      661 gatcgcccgg cggcgtaccg aggcgacggc gcgagcgctg gccaacgcgc cggccgccgt
      721 gcgcggcgtg gtcggcactt ccggcacggc catcgatacc gtgctcgccg ggacgcccaa
      781 gctgaacatc atcgagcgcg agccctctgc cgacgtgcag cgcgagaagt cctggttgac
      841 ggctccttcg acaggggcgc gccatctggc cctcgtcgtc gttcacccgg acgctgcgga
      901 agccagggcc gggtccgact tcggcagcta cgacgtgtac gttccgccga acctggacga
      961 acgcgtcgag aacaccctgt acgaggggtt ccgcgaggcc gtcctcaccg ctcgaatccg
     1021 cggacgtcaa ctggaccggg cgcaggtcga gaccgtcatg cgcgtgacga ggccgacgtc
     1081 ggtgacggtg accgctgaga acgaacggcg aaccaatgtc ggattcaatc gctcgctgcc
     1141 gttcatcttc ggcggtctcc tcgtcttcgg catcatgatt ggcggccaga cgctgctgac
     1201 ctcgctcatc gaggagaagt cgagccgcgt cgtcgaggtg ctgctctcgg cggtgtcgcc
     1261 gctcgagctc atggcgggga agatcctcgg gcagatggcc gtcagcatgc tcgttctggc
     1321 gctctatctt ggcgcggggc tcgcgctgct gacgtcgttc gcgatgatcg gactgctcga
     1381 tccgatgctg atcgtgtacc tcttgatctt cttcgtgatc tcctacctgg tgttcgcggc
     1441 ggccttcggc gccgttggcg ccgccgtcaa cgagatgcgc gaggcgcagt cgctgatgac
     1501 gcccgtgatg ctggcgctca tgtcgccgtg gatgctgggc ccgatgatcg ctcgcgagcc
     1561 caactcgacg tttgcggtgg ccgcgagctt catcccgccg gtgaatacct tcgcgatgat
     1621 gacccgcctc gcgtccagca gtccgccgcc ggcgtggcag gtctgggcga cggtcgcggt
     1681 cggcctggcc tcggcgtttg ccgtcacctg gttcgcggcg aaggtcttca aggtcggcct
     1741 gctcatgcac ggcaaggcgc ccagcttctc cacgctcgta cgctgggcga gagaggcgtg
     1801 atcgcgaggc tcgaatgccg atctgggtgg gcacgtccgg ctacaactat cccgagtgga
     1861 agggaagttt ctatcccgag aacctgcccg cggcgaagat gctgccgtac tacgccgagc
     1921 gttttccgac cgtcgagatc aactacacgt tctaccgcgc tcccaacgag aagatcctcg
     1981 agggctggaa caaggccacg cccgatcggt tcaagctgac gctgaaggcg ccgcggcgca
     2041 ttacccacga cgcccgtctg cgtgactgcg ccgatcgtgt ccggcagttc ctcgagacgt
     2101 cggcaaacct cgggccgaaa ctgggtgcgc tgctgttcca gctcccgcca aacctgaaga
     2161 aggacctcgc gctcttcgac cttttcctgg aggcgtttcc tcctcgcgtc tgcgccgcct
     2221 tcgagttccg gcacgagtcg tggctcgacg cagaggtgta cgagcggctg cggtcgcgga
     2281 acctggcgct ctgcgtggcg gacagcgagc ggctctccac tcccgtcgag atcaccgcgg
     2341 attatgccta cctgcggctc cgtgatgaag gctacacgac ggacgacatt gcccgatggg
     2401 cctcgacgat ccgggagcgg gcctcaggat gcaaggacgt gttcgtctac ttcaagcacg
     2461 aggaagaggg gaaggggccg gagtttgcgc ggacgctgat cgaccatctc ggtgcgccgt
     2521 gacgaacctg acgacttctc tctccgccca gtagccgtcg taactccccg acgcgtgctc
     2581 gagaaacgcg cagtgtccgc catgacgcgt cacgacggtc gtcacaagtg gatttccggt
     2641 caccgagtgg tgggcgaacg gctcgaccgg cacgaagggg tcgtcttcgg ccgtgacgat
     2701 cagggcgggg acgctgatcc ggtcgatgac tctcatggcg ctcgcacgga agtagtagtc
     2761 ggcggcgtcc ctgaagccgt gatggggcgc tgtataggcc tcgtcgaact ggcgcaccgt
     2821 ccagattcgc cgcagcggct ccagcgggaa cagcccaggg acgatcgcgg ccttgcggcg
     2881 cattcgcgcc ttcagccgcc ggacgaagtt ccactgatac gggatattcg ccggtcgttc
     2941 gagcgccttc acgcagacgg ccagatccat ggtgggggag acagcgcaga tcgctttcag
     3001 ctccggaggc gcgctctcgc cgaagtctcc cgccagcttg agcgcgaggt ttccgcccag
     3061 cgagtagccc gcgaccgcga tcgcgcggat tcggtcccgc tcgacgagct cacggatcac
     3121 gaaccgcggg tcgtcggtca gccccgagtg gtacaggccg cgtgagaggt gctcggtccc
     3181 tccgcagttc cgctggttga gccgcacgac gttccacccg agtgcccacg ccttgtccgc
     3241 cattccgccc atgtagtgga cgaggctcga accttcgagc ccgtggagca gcacgagggt
     3301 cggatgttcc accgcgcgat cctgccaatg gcagtgcgcg aggacgcgcg catccggcgc
     3361 cacgtcgaag aagcgttcgg aaggcggcgg aagccgcgga aacgtcctcg gtcgggccca
     3421 ggcgtaaagc gtttggacgt ggccgttggt aaagcgcggc cggaagcggg aggcggtgaa
     3481 agtcacgcag tcgtgcggcg attggccccg gaacgggccc cgatgtgagc ttatcatttg
     3541 acctaaccga ggactcgctt gtgttcggtg gtattgcata cgcccggccc gactggactg
     3601 acggtggttt cgcatagttg gaggctgaag cttccaaaca caagcgagct gtagggtaag
     3661 ctaggcagca ctttcacgca ggagactccc catgacgcgc atggctacct tcgtcggcgc
     3721 cctgtccatc gcggcttccg tcggcgtcgg cacgctggcg caggagccgg cgccgccgcc
     3781 gcaggacact cccgcgcaga cgccggggca gacgccgggg cctcctggtc gcggcggagc
     3841 gaaccaggac ccgcggccgt acgaacaggt gatcaccaag gaggccaaga gcgataccgg
     3901 cgtgttcacg gtgcatcgca ttcgcgaccg catctactac gagattccga agagcgagct
     3961 gggcaaggaa ttcctgtggg tcagccagat cgcgcgcacc acgctcggcg ccggatgggg
     4021 cggccaggcg gtgagcgacc gcgtcgtgaa gtgggaacgg ctgaacaacc gcgtcctgct
     4081 caagggcatc gcctacgacg tcgtcgccga tccgttgtcg ccaatcgggc gggcggtgca
     4141 ggccgccaac aacgacacga tcatcgcgtc tttcaacgtc gaggccgtgg ggaaagacga
     4201 ggcgccggtc atcgaggtca cgcggctgtt cacgaccgac gtccccgagt tcagcgcgcg
     4261 tgctcgcctc cgcgggcggg gaatggacgc cagccggtcg ttccttgatc gcgcgtcctc
     4321 gtttccccag aacatcgaag tcgaggcgac gcacacctac accgccggga cggacgctcc
     4381 cgccggcgcg gcgcccgtgc agccctccgc gattcgcggc gggagcggca cggtcgtcat
     4441 gcacttcagc atggtcaagc ttccggaaaa gccgatgatg ccccggctct tcgatgatcg
     4501 ggtcggctac ttctcgattc agaagtacga ctacagcctg gaagagcatc gcgccccgcg
     4561 ccgccgctac atcacgcgat atcggctcga gaagaaggac ccgggtgcgg ccctgtcaga
     4621 gccggtgaag ccgatcgagt actggataga cccggcgacg cccaccaagt ggattccgtt
     4681 cgtgaagaag gggatcgagg cgtggcagcc ggcgttcgag gcggccggct tcaagaacgc
     4741 cattgtcgcc agggacgcgc cgagccccgc cgaggacccc gactggagcc cggaggacgc
     4801 gcgctactcg gtcatccgat ggctgccgac gaccaccgaa aacgcgtccg ggccgcacat
     4861 ccacgacccg cgcacagggg agatcctgga ggcggacatt cagatgcatc acaacgtgat
     4921 gaacctggtc cgcgactggt acttcgtcca ggtcgggcac ctcgacccgc gcgcgcagaa
     4981 gctgccgctg ccggacgcgc tcatgggacg gctcgtcgag ttcgtcgtgg cgcacgaggt
     5041 cggccacacg ctcggcttcc agcacaacat gaaggcgagc tcgacctatc ctgccgacaa
     5101 ggtccgcgat ccggcctggg tgaagaagat ggggcacacg ccgaccatca tggactactc
     5161 gcgattcaat tacaccgcgc agcctgaaga ccggatcgcc ctggaggatc tggttcccgg
     5221 catcggtccg tacgacaagt gggcgacgca ctggggctac gcgccgattg ccggcgccgc
     5281 cctgcccgat gacgagaagg caacgctcga ccgctggtcg cgcgagcagg actcgacgcc
     5341 gtggctccgc ttctcgacgg ccgacgcgca gggcgtcgat cccggcgagc tgaccgaagc
     5401 ggtcggcgat gccgatgcga tccgcgccag caccctcggc atgaagaatc tccagcgtgt
     5461 cggcgccatg ctgctcaccg ccacgacggc gaagccgggt gagccgtacg acgacctcga
     5521 agagctgtac ggccgcctgc tcggccagtg ggtgctcgag atgaatcacg tggcggccct
     5581 catcggcggc ttcgacaccc ggcagaagca cgccggccag gacggcgtgc ggttcacgat
     5641 cgtgccgaag caacgtcagg cggccgcggt ggcgttcctc aacgccaacg ctttcacggt
     5701 tccgtccttc gccgtgcagc ccgagatcct gcgccgcatc gaaccgagcg gcgtgcttca
     5761 gcgcgtgcag accgcgcagc tccgcgtcct cacgtcggtg ctcacgccgg cgcgctttgc
     5821 gcgtctcatc gaacaggagg ccctcgacgg cgccgccgca tacaaggcca ccgacttcct
     5881 ccgcgatctg agacggggga tctggcgcga gctggggtcc tcccaggtgg cgatcgacgc
     5941 ctaccgccgc ggcacgcagc ggctgttcct cgacgccatg gccgagcgtc t
//
LOCUS       DBNGSH010000380         1857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2750531, whole genome shotgun sequence.
ACCESSION   DBNGSH010000380 DBNGSH010000000
VERSION     DBNGSH010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2750531"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="AC16CY_08820"
     CDS             complement(<1..525)
                     /locus_tag="AC16CY_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3353929.1"
                     /translation="MLRLLRIRNLAVIEEVEVELEPGFNVLTGETGAGKSILVEAVGL
                     LLGGRASADLVRTGAQQAVIEAVFEDASDGETIVRREVTSQGRSRAFINDSLATAAAL
                     RDLSDRLIELHGQHEHQALLDPLSHLSVLDESAGLLPLGGQVASAWESVSSLREARDR
                     ARMDEREKAARLDLL"
     gene            638..1390
                     /locus_tag="AC16CY_08825"
     CDS             638..1390
                     /locus_tag="AC16CY_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR3353930.1"
                     /translation="MYTFGSSILALQVQDAAPPAGMGGLLQIVADSSVLSQIVLGILI
                     FFSITSWAIILFKTLSYRAVARQTATFLEVFRKSQKFSDVQAVCPSLPSSPLVGVFQA
                     GYAELTAQFRLSQPATGSPGTSGARPILKSLDAVDRALIRAATTEVSRLERRLTFLAT
                     TASVTPFIGLFGTVVGITLAFQRIGATGSTNLTVVGPGISEALIATAAGLFAAIPAVV
                     SYNHFVHRVKDFAAAMDDFALEFLNIAERNFT"
     gene            1390..1851
                     /locus_tag="AC16CY_08830"
     CDS             1390..1851
                     /locus_tag="AC16CY_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3353931.1"
                     /translation="MPKVMGSAPDAGGRHQRGRRVGPTLAEINVIPLVDVMLVLLIIF
                     MVAAPMLQRGVEVSLPEAIKAQEIESEPIYIDVPLSFGKDRRVFLGDEPIRLGALPER
                     VRQLTLNQTTKRVFLRIDRGLTVQHEIDVFVELMGAGIRQVGFVTDPPKRR"
BASE COUNT          310 a          612 c          583 g          352 t
ORIGIN      
        1 gagcagatcg agacgcgccg ctttctcgcg ctcgtccatg cgcgcacgat cgcgcgcctc
       61 ccggagcgac gagaccgact cccaggccga ggcgacctgg ccgccgagcg gcaggagccc
      121 ggccgactcg tccagtacgg agagatggga caacggatcg agaagcgcct ggtgttcatg
      181 ctgtccgtga agctcgatga gccgatcgga gaggtcacga agggcggccg ccgtcgccag
      241 cgagtcgttg atgaacgccc ggctccgccc ctggctcgtg acctcgcggc ggacgatcgt
      301 ctcgccgtca gaggcgtcct cgaagaccgc ttcgatgacg gcctgctgtg ctccggttcg
      361 aaccagatcc gcagatgctc gaccgccgag caggagcccg acggcttcaa cgaggatcga
      421 cttgcccgcg cctgtctcgc ccgtcaggac gttgaagccg ggctcgagct cgacctcgac
      481 ctcctcgatc acagcgaggt tgcgaatccg caggaggcgc agcatgcacg cggaacttcc
      541 atcgtatcat acgtttgaca gccctcaggc gccgtgctag tctgccttgt ctgcgctgtc
      601 ccagcggcgc atcccccaca atggaccgga ggcttcggtg tatacgtttg gctcgtccat
      661 ccttgcactg caggtgcagg acgcggcccc gcctgctggg atgggcggcc tgctgcagat
      721 cgtcgcggac tcgagtgttc tcagccagat cgtccttggg atcctgatct tcttctcgat
      781 cacgtcttgg gcgatcattc tcttcaagac actttcctat cgcgcggtcg cgcggcagac
      841 ggcgaccttc ctcgaggtct ttcgcaagag ccagaagttc tccgacgtgc aggcggtgtg
      901 tccgtcgctt ccgagcagtc ctctggttgg agtctttcaa gccgggtacg ctgagctcac
      961 ggcacagttc aggctgagcc aaccagccac cggcagtcca ggcacctcag gagcgcgtcc
     1021 aatcctcaag agcctcgacg ccgtcgaccg cgcgctgatc cgcgctgcca cgaccgaggt
     1081 ctccaggctg gagcgacggt tgacgtttct cgcgaccacg gcgagcgtga cgcccttcat
     1141 cggcctgttc ggaaccgtgg tcggcatcac gctcgcgttt cagcgaatcg gcgcgaccgg
     1201 ttcgacgaac ctcaccgtcg tcgggcccgg tatcagcgag gcgctcattg cgacggcggc
     1261 cggcctcttc gcggcgatac ccgccgtcgt ctcttacaac cacttcgtgc atcgagtgaa
     1321 ggatttcgcc gccgccatgg atgacttcgc tctcgagttt ctcaacatcg ctgagcggaa
     1381 cttcacctga tgccgaaggt catgggctcg gcgccggatg cgggcggccg tcaccagcgc
     1441 ggccgtcggg taggcccgac gcttgcagag atcaacgtca tccccctcgt cgatgtgatg
     1501 ctggtgctgc tcatcatttt catggtcgcg gcgccgatgc tgcaacgcgg cgtcgaagtg
     1561 tcgcttccgg aagcgatcaa ggcgcaggaa atcgagagcg aacccatcta tatcgacgtc
     1621 ccgctgtcgt tcgggaagga ccgccgtgta tttcttggag acgagcccat ccgtctgggg
     1681 gcgctgcccg aacgcgtccg gcagttgacg ctcaaccaga cgacgaagcg ggtgttcctg
     1741 cgcatcgatc gaggactgac ggtgcagcac gagatcgatg tgttcgtcga gctcatgggc
     1801 gccgggatcc gccaggtcgg tttcgtcact gatccgccga aacgccgttg agatgac
//
LOCUS       DBNGSH010000381         1942 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1053194, whole genome shotgun sequence.
ACCESSION   DBNGSH010000381 DBNGSH010000000
VERSION     DBNGSH010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1053194"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="AC16CY_08835"
     CDS             complement(<1..938)
                     /locus_tag="AC16CY_08835"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3353932.1"
                     /translation="MKIDIFNHVFPKKWFERYISSGAAGREIGKRMANATVGVDLDHR
                     FRILDEFGDVRQVITLGQPPIEMLGGPAESPAVAREANDGLADLVARHPDRFIGFGAS
                     LPLNNPDASLEEMRRAIDQLGAQGVMIYTNVAGKPLDAPEFQPLFDEAARRGLPIWLH
                     PARGASMTDYKSEETSAFEIWWALGWPYETSAAMARLVFSGVFDRHPDLTIITHHLGG
                     LVPYLEGRVGYGWDLLGARTSTVDYSTLLRSMKKRPVDYFRQFHADTAMFGARAATRC
                     GIEFFGVDRVLFASDMPFDPTPGLYMRETIRVIDSL"
     gene            complement(935..>1942)
                     /locus_tag="AC16CY_08840"
     CDS             complement(935..>1942)
                     /locus_tag="AC16CY_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3353933.1"
                     /translation="TAQSVEGLAAFRALTGLGMGGAMPNAIALTSEYMPRRRRGTAVT
                     WMICGFSLGAAVGGFVAAEIIPRFGWEAVFVVGGVAPILIAGAAFLWLPDSIRFLVVK
                     GGEDERARRYLQRIAPGVALPPALSAGVDEDHSGGAFVVRQLFTERRAAGTVLIWVVY
                     FMNLLNLYFLNSWLPTIISDAGIPVETANRLTALFQLGGIGGALILGAVLDRIFSFWI
                     LAGCYLWAAGSVYLIGTAASIPALAITITCAGLAIIGGQNACHAVTAEFYPARIRSTG
                     VGWALGIGRIGSIVGPVVGGLLLAQGTPIGQVFWAATIPALVASSAAAGVAMAGRGES
                     K"
BASE COUNT          357 a          679 c          630 g          276 t
ORIGIN      
        1 tcgaggctgt cgatgacgcg gatcgtctcg cgcatgtaga ggcccggcgt cggatcgaac
       61 ggcatgtcgc tcgcaaacag cacgcggtcc acgccgaaga actcgatccc gcagcgcgtc
      121 gccgccctgg cgccgaacat cgccgtgtcg gcgtggaact ggcggaagta gtcgactggc
      181 cgcttcttca tcgaccgcag cagcgtcgag taatcgaccg tggacgtgcg cgccccgagc
      241 agatcccagc cgtagccgac gcgcccttcc aggtacggca cgagcccgcc aaggtgatgc
      301 gtgatgatcg tcaggtccgg gtggcggtcg aacacgccgg agaacaccag gcgggccatc
      361 gcggcgctcg tctcataggg ccatccgagc gcccaccaga tctcgaaggc cgacgtctcc
      421 tccgacttgt agtccgtcat cgacgcgccg cgcgcgggat gcagccagat cggaagcccg
      481 cgccgtgcgg cttcgtcgaa gagcggctgg aactcgggcg cgtcgagcgg cttgccggca
      541 acgttcgtgt agatcatcac gccctgtgcg ccgagctggt cgatcgcgcg cctcatctcc
      601 tcgagcgagg catccgggtt gttgagcggc agggacgcgc cgaagccgat gaatcgatcc
      661 ggatgccggg ccacgagatc cgcgagaccg tcgttggcct cccgcgccac ggccggagac
      721 tccgcgggcc cgccgagcat ttcgatcgga ggctgaccga gcgtgatcac ctggcgcacg
      781 tcgccgaact cgtcgagaat cctgaagcga tggtccagat cgacgcccac ggttgcgttc
      841 gccatgcgct tgccgatctc cctgcccgcg gctccgctgc tgatgtaccg ttcgaaccac
      901 ttcttcggaa acacgtgatt gaagatgtcg atcttcactt cgactctccg cgcccggcca
      961 tcgccacgcc ggccgcggcg gacgaggcaa cgagggctgg aatggtggca gcccagaaga
     1021 cctggccgat cggcgtgcct tgcgccagga gcagcccgcc gacgacgggt ccgacgatcg
     1081 atcctatccg gccgatgcca agggcccagc cgacgcccgt cgagcggatc ctggccgggt
     1141 agaactcggc cgtcaccgcg tggcacgcat tctgcccgcc gatgatagcc aaccccgcgc
     1201 acgtaatcgt gatcgccagc gccggaatcg acgcggccgt gccaatgagg tacacgctcc
     1261 ccgcagccca gagataacag cccgcgagga tccagaagga aaaaatgcga tcgagcacgg
     1321 cgccgagaat caacgcacca ccgatgccgc caagctggaa gagcgccgtc agccggttgg
     1381 ccgtctccac gggaatgccc gcgtcgctga tgatggtcgg cagccagctg ttcaggaagt
     1441 acaggttcag aaggttcatg aagtagacga cccagatcag caccgtcccg gcagcccggc
     1501 gctccgtgaa gagctgccgg acgacgaagg cgccccccga gtggtcttcg tccacgcccg
     1561 ccgacagcgc gggcggaagc gcaacacccg gggcgatccg ctgcaggtaa cgcctggctc
     1621 gctcgtcctc tccccccttc acgacgagga agcggatgga atcgggcagc cagagaaacg
     1681 cggcgccggc aatcaggatg ggcgcgacac cgcccacgac gaacaccgct tcccatccga
     1741 atcggggaat gatctcggcg gcgacgaacc ctcccacagc agcgcccagc gagaagccgc
     1801 agatcatcca ggtcacggcc gtgcctcgcc gccggcgcgg catgtactcg gacgtcagcg
     1861 cgatggcgtt cggcatggcg ccgcccatgc cgaggccggt gagcgcccgg aacgcggcca
     1921 gcccttccac cgactgtgct gt
//
LOCUS       DBNGSH010000382         2885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_34503, whole genome shotgun sequence.
ACCESSION   DBNGSH010000382 DBNGSH010000000
VERSION     DBNGSH010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_34503"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="AC16CY_08845"
     CDS             <1..183
                     /locus_tag="AC16CY_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353934.1"
                     /translation="RRQHAELVLYIRDTTDDLRGRIVERQRSDAYQWALLISTHEQRH
                     ILQIREIKGDPRYPRK"
     gene            complement(547..1521)
                     /locus_tag="AC16CY_08850"
     CDS             complement(547..1521)
                     /locus_tag="AC16CY_08850"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3353935.1"
                     /translation="MEATTLPIFRDQLLDRRQQLETVAATLGRPDEVTRLLHEVDAAL
                     GRMNAGTFGVCATCHEAIEPDRLLTDPLTQFCLDHLTPVEQRALQQDLDLASRIQREL
                     LPGPDVRLDGWEVAYHYQPAGPVSGDYCDLIPSNADQGYFLIGDVAGKGVAAAMLMSH
                     LSALLRTLITIGLPLSQLMERASRVFCESTLPTHYATLVCGRASSSGEIEICNAGHPP
                     PLLVHAGEIIRFDATGLPIGMFCSEQFSSRIVRLARGDTLLLYTDGLLEAQNAAGVEY
                     GIERLAALAAAANRQPKVIVDTCLRDLAGFRGAPGADDDLTILAISRV"
     gene            complement(1839..>2885)
                     /locus_tag="AC16CY_08855"
     CDS             complement(1839..>2885)
                     /locus_tag="AC16CY_08855"
                     /inference="COORDINATES: protein motif:HMM:NF018647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3353936.1"
                     /translation="VRYWCLGNEMDGPWQIGQMQSREYGRKARDVARQMRGIDRGVQL
                     IACGSSGTNMPQYLAWDREVLEECFDQVDGISLHAYYGNTQALTRNSAARYLAMNLDM
                     DRQIREIAAVCDYVQALRRSSKRLWLSFDEWNVWYRARSGDAVDGRRAFAPKLLEEVY
                     NLEDALLVGGFVNTLLRNSDRVRVGCLAQLVNVIAPLVTNETGVLRQSIYFPYAWALR
                     YARGRVLDVRVEAETYPITAAGLQADFARNDQVPFVDLVATHDAQNRQASVLMLNRDL
                     DAEREVVLEWGEVTPTKVLACETLTGADLKAFNTFEQPRRVAPQRLEAPAAGTRMAFR
                     LPPRSYTVAQLATM"
BASE COUNT          488 a          982 c          919 g          496 t
ORIGIN      
        1 cgcaggcagc acgccgagtt ggtcctgtac atccgcgaca ccaccgacga cctgcgcgga
       61 cggatcgtcg agcgccagcg ctccgacgcg tatcaatggg ccctgctgat ctccacgcac
      121 gagcagcggc acatcctcca gatccgcgag atcaaagggg atcccaggta tcccaggaag
      181 tagtcgtcat gccttgcgct gcagtgcgtc ggctgcatcg cactgatgcc gcttggcgat
      241 tttctagcgg cgtcgacgct cacccgcgcg ggcggcatcg ggtgaattca caacgagtct
      301 gtctgagttc ctcacgatca gccgagagca cgaactcgag gtagacgagc ggcacacctc
      361 gataaacggc gcgcccgacc tcgctgaacc ccactgccgg gccacgcctt tcgcacgttc
      421 aataatctga cgtccaatac ctgatcgctg cagccgtggc tcccggctca ccccgcactc
      481 aaccgcggcg cgcgtgatcc accgtaccgt cggttcctgc gctgagaacg cctgcgcagg
      541 cggagcctac acacggctga tggcgaggat cgtgagatcg tcatccgctc ccggggcccc
      601 gcggaacccg gccaggtcgc gcaagcatgt atcgacgatg acctttggct gccggttcgc
      661 tgccgccgcc agtgcagcca ggcgctcgat gccgtattcc acgccggccg cattctgcgc
      721 ctcgagcagg ccatcggtat atagcagcaa cgtgtcgccg cgtgcgagcc gcacgatccg
      781 cgatgagaac tgctcgctgc aaaacatgcc gatcggcaag cccgttgcgt cgaaccggat
      841 gatctcgccc gcgtggacga gtaacggcgg cggatggccc gcgttgcaga tttcgatctc
      901 gcctgacgag gacgcgcggc cacagacaag cgtggcgtag tgcgtcggca gcgtgctttc
      961 acagaagacg cggctcgcgc gttccatcaa ctggctgagc ggaaggccga tagtgatgag
     1021 cgttcgcagc agcgcgctca agtgcgacat cagcatcgca gccgcgactc ccttgccggc
     1081 gacgtcgccg atcaggaaat acccttggtc cgcattgctg gggatgagat cgcagtaatc
     1141 gccgctgact gggcccgcag gctgatagtg ataggcgact tcccacccat cgaggcggac
     1201 gtctgggcct ggcagcagtt ctcgctgaat acgcgaagcg agatcgaggt cttgctggag
     1261 cgcgcgctgc tcgacgggcg tcaagtgatc caggcagaat tgcgtcagcg ggtctgtcag
     1321 cagccggtcg ggctcgattg cctcgtggca cgtcgcgcag acaccgaacg tgcccgcgtt
     1381 catgcgtccc agcgcggcgt cgacttcgtg cagaaggcgc gtcacttcgt ccggtcggcc
     1441 aagggtggcg gccacggttt ccagttgctg acgtcggtcg agcaactgat cacggaagat
     1501 tgggagtgtg gtcgcttcca tggccagacc tctatgcgat atcgagagga gcctttggat
     1561 gaggcggtct cttcgaaagt tctcttcgca ccattcgacg cgcgagggcc cgatccgatc
     1621 aggccgccca acgccttacg gagtagaccg acacgagcct tcgccgcgta caacgaccgc
     1681 gataggcccc tcccgctgcc accatctatc aggcgcgccg gaggatagca cgcacccgtc
     1741 ttcccgtccc ggcattgacc cgcgagccgg caatgggccg ccggccacgc gggctatttt
     1801 cagccacgct cgcccgccag atggcgtcgt gactctcgtc acatcgtcgc gagctgagcc
     1861 accgtgtagg aacggggcgg cagcctgaac gccattcgcg ttcccgccgc cggcgcctcg
     1921 agccgctggg gcgcgacgcg gcgcggctgc tcgaatgtgt tgaacgcttt cagatcggca
     1981 cctgtcagcg tctcgcatgc cagcactttc gtcggcgtca cctctcccca ctccaggacc
     2041 acctcgcgct ccgcgtccag gtcgcggttc agcatcagca cgctcgcctg gcggttctgc
     2101 gcgtcgtgcg tcgcgacgag atcgacgaag ggcacctgat cgtttctcgc gaagtccgcc
     2161 tggaggcccg cggcggtaat tggatacgtc tccgcctcca cgcgcacgtc gagcacgcgg
     2221 ccccgtgcgt agcgcagcgc ccaggcgtac gggaagtaga tgctctggcg caggacaccg
     2281 gtctcgttgg tcacgagcgg cgcgatgacg ttgacgagct gcgccaggca gccgacgcgg
     2341 acgcggtccg agttcctgag cagcgtgttg acgaagccgc cgacgagcag cgcgtcctcg
     2401 aggttgtaga cctcctcgag gagcttcgga gcaaacgcgc ggcggccgtc aacggcgtcg
     2461 ccgcttcggg cgcggtacca gacgttccac tcgtcgaacg acagccagag gcgcttgctc
     2521 gatcgccgga gcgcctggac gtagtcgcac accgccgcga tctcgcggat ctgccgatcc
     2581 atgtcgaggt tcatcgccag gtaccgcgcg gcgctgttgc gcgtcagcgc ctgcgtgttg
     2641 ccgtagtagg cgtgcaggga gatgccgtcc acctggtcga agcactcctc cagcacctcg
     2701 cgatcccagg cgagatactg cggcatgttg gtgccgctcg atccgcaggc gatgagctgc
     2761 acgccgcggt cgatgccgcg catctgtctc gccacgtcgc gcgccttgcg cccgtactcg
     2821 cgggactgca tctggccgat ctgccagggg ccgtccatct cgttgcccag gcaccagtag
     2881 cgcac
//
LOCUS       DBNGSH010000383         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4279043, whole genome shotgun sequence.
ACCESSION   DBNGSH010000383 DBNGSH010000000
VERSION     DBNGSH010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4279043"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="AC16CY_08860"
     CDS             complement(<1..448)
                     /locus_tag="AC16CY_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353937.1"
                     /translation="MKRPNRAVFVVTAVVALTVTVAHGQGQQGGRGDAPGALVAGRGG
                     GRGLAPVMTRPKALIPNAAPVRSCESLASVALPNTTIESAVVDAGSQVCRVTAITTHP
                     PAGDKVRIWVAIPTTNWNGRFLGNGGGGFSGGTDTSVVAPAAAGYAA"
     gene            complement(529..1482)
                     /locus_tag="AC16CY_08865"
     CDS             complement(529..1482)
                     /locus_tag="AC16CY_08865"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3353938.1"
                     /translation="MAPKPPDLNLLPIAVALYDELSVSRAARQLGMSQPAVSKALRRL
                     RETFDDPLFVRGPSGIVPTPRAHAIVRAARPHLQHLQEDLLKGERFDPATSTRPIVLG
                     LSDIAEMAFLPSILEYLRVHAPKCPVTTMTVPDAQLAEALERGDVDVAAGYFPALAQR
                     NFRQRRLSKHGFACLMRAGHPLWASRLTVSAFLAAEHVVVRRGGRSQDILERFIERRK
                     MRRKVAMYTSNVLSVPFIVMDTQLIATLPYAVVTRFTSLTSQVAAALPPFDLTYDLKL
                     HWHRRFDNEPRSIWLREQLTAVFKDHHWLQPPTGPAPFREA"
     gene            complement(1519..>1992)
                     /locus_tag="AC16CY_08870"
     CDS             complement(1519..>1992)
                     /locus_tag="AC16CY_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine aminopeptidase domain-containing protein"
                     /protein_id="HIR3353939.1"
                     /translation="APARGRGALADVNDPPNLRELSEKDVMYVLSLVRQEFNVDERRI
                     YLMGHSMGGAGTFHLALKYPGNWAATASIAPAAFGMQPSSLSAIASMPMMIVHGDADT
                     VVPVDVGRAWAEAMKERKMTHQYIEVPKGDHGNVITIGMPDIFAFFAKHSKPVSR"
BASE COUNT          307 a          715 c          650 g          320 t
ORIGIN      
        1 ccgccgcata acccgccgcg gcgggtgcga cgacgctcgt gtcggtgccg cccgagaacc
       61 ctccgccgcc gtttccaaga aagcggccgt tccagttcgt cgtcgggatg gcaacccaga
      121 tccgcacttt gtctcccgcc ggtggatgcg tggtgatggc ggtgacgcgg cagacctggc
      181 taccggcatc gacgacggcg gactcgatcg tcgtgttcgg cagcgcgacc gacgcgaggc
      241 tctcgcacga tcgcaccggc gcggcgttgg gaatcaacgc cttcggccgg gtcatgaccg
      301 gggccaatcc ccgcccgccg ccacgcccgg cgacgagggc gcccggcgca tcgccgcgac
      361 caccctgctg gccctggccg tgtgcgacgg tcaccgtcaa cgccacaaca gccgttacga
      421 caaacaccgc tcgattcggt cgcttcatcc ttccccctgc cccctgcccc ctgccccctg
      481 cccccttccc cttgcccccg gcccccgtcc ccttgccccc ttcccccttc aagcttcccg
      541 gaacggcgcg gggcctgtcg gcggctgcag ccagtggtga tccttgaaca cggcggtcag
      601 ctgctcgcga agccagatgc tcctcggctc gttgtcgaat cgccggtgcc agtgcagctt
      661 caagtcgtac gtcaggtcga agggcggcag cgccgcggcc acctgcgagg tcagcgacgt
      721 gaatcgcgtc acgaccgcgt acggcagcgt ggcaatgagc tgcgtgtcca tgacgatgaa
      781 cggcacgctc agcacgttcg acgtgtacat cgcgaccttc cggcgcatct tccggcgctc
      841 gatgaagcgc tcgaggatgt cctggctgcg gcccccgcgg cggacgacaa cgtgctcggc
      901 ggcgaggaag gcggacaccg tcaggcggct cgcccacaac ggatgcccgg cgcgcatcag
      961 gcaggcgaac ccgtgcttcg acagccggcg ttgacggaag ttccgctgcg ccagcgccgg
     1021 aaagtatccg gccgccacgt ccacgtcgcc tcgttccagc gcttccgcca gctgcgcgtc
     1081 cggcaccgtc atggtggtga cggggcactt cggcgcgtgc acgcgcaggt actcgaggat
     1141 cgacggcaga aacgccatct ccgcgatgtc ggagagcccg aggacgatcg gccgcgtgct
     1201 ggtggccggg tcgaagcgtt cacccttgag cagatcttcc tggagatgct gcaggtgcgg
     1261 ccgcgccgcg cggacgatcg cgtgcgcccg cggcgtcggg acgatgccgc tcggcccgcg
     1321 gacgaagagc gggtcgtcga acgtctcgcg cagccgccgc agcgccttgc tgacggcagg
     1381 ctggctcatc ccgagttgtc gcgccgcgcg gctcacactc agctcgtcgt agagagcaac
     1441 cgcgatgggc aacagattga ggtcgggggg cttgggtgcc aaccgcttcc accgcccgag
     1501 agtctacggc actccgcctc agcgcgagac cggcttcgag tgcttggcga agaacgcgaa
     1561 gatatcgggc atgccgatcg tgatcacgtt gccatgatcg cccttcggca cctcgatgta
     1621 ttggtgcgtc atcttccgct ctttcatcgc ctcggcccag gcacgcccga cgtccacggg
     1681 aaccacagtg tcggcatcac cgtggacgat catcatcggc atgctcgcga ttgccgaaag
     1741 cgaggagggc tgcatgccga acgccgcggg cgcgatcgac gccgtggcgg cccagttgcc
     1801 cgggtatttc agcgccaggt ggaaggtgcc ggccccgccc atcgaatggc ccatcaggta
     1861 gatgcggcgc tcgtccacgt tgaactcctg gcggacgagc gagagcacgt acatcacatc
     1921 cttttcgctc agctcgcgaa ggttgggcgg gtcgttgacg tcagccaacg ctcccctgcc
     1981 ccgagccggt gc
//
LOCUS       DBNGSH010000384         2983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2581166, whole genome shotgun sequence.
ACCESSION   DBNGSH010000384 DBNGSH010000000
VERSION     DBNGSH010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2581166"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC16CY_08875"
     CDS             complement(<1..365)
                     /locus_tag="AC16CY_08875"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR3353940.1"
                     /translation="MALLPCLVEVLKRERVPYLTLRHPPAFTAPELSAVAHIPGRRSA
                     KVVICIADSQPVQAVLPAHYTVDLEALRRLAGAEALRLAHEEEIAALYPEFEVGAMPP
                     FGMMKGHRVFVERCFVGEP"
     gene            520..>2983
                     /locus_tag="AC16CY_08880"
     CDS             520..>2983
                     /locus_tag="AC16CY_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011129920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3353941.1"
                     /translation="MKSRPRYPGVRVTANGNQLVSYHTETRIADAGVFYPITPSTEGG
                     ELYQQAFAEGKLNAFGRNTIAIEAEGEHAAQGGAIAHSVCGKRVVNYTSGQGVVYGVE
                     QYYHAPGKCSTMVLEVAARALTKHALNVHCGHDDVYGALDTGWIMVFGKDAQQAADQA
                     LILRRVTELSLTPGMNVMDGFLTSHLERTFYKHESELIREYLGAPEDIIDCPTESQRI
                     LFGPTRRRVPKMIDLTNPVLLGPVQNQEHYMQGVVARRDNFTEPILQFFEAAYEDFAK
                     LTGRYYGLVSEYKTEDTDTVFVSLGSAAENVEAAVDYLRERRGVTVGSIHINVFRPFP
                     DAAVVKALTGKKNVIVLERTDEAMAGDNPMGRDIRTALHKALQGEAGLPKITADQMPR
                     LFGGVYGLGSRDFRPEHTIGAYEFATGQRARKDGKRASDGASFMVLGVDHPYEVKSDD
                     TPSLLPDKAIAVRFHSVGGWGAITTGKNLGAIIGDLNDLLYERDKIVDEFGNPKEIIH
                     VSANPKYGSEKKGAPTSYFMIAAPERIRVNCDLRHVNVVLCCDPKAFTHTNPLDGMSD
                     GGCLVWESDEEGEAAWERLPLWARKQIIDKHIRVFTLPGFKIAREATDRGDLQLRMQG
                     NAFLGAFFSVSPMLQDFRITPEQFRDAVHKQYVKKFGKLGEGVVNSNMEVMTKGFELV
                     REINVGALEAPDRSTLRGRPLLPVMMPESLAASEGCGTGCRTIPLPDGQNERTPLTRV
                     GEFDRMFRAKLGYNQPANAYASLGIMAAGSGDTASKYVARRETPLYIPENCTQCMECI
                     AVCPDTALPNCSQDLDTILYTAV"
BASE COUNT          555 a          995 c          973 g          460 t
ORIGIN      
        1 tccggctcgc cgacgaagca ccgttcgacg aagacgcgat ggcccttcat catgccgaac
       61 ggcggcatgg cgcccacctc gaattccggg tacaacgccg caatctcctc ctcgtgcgcc
      121 agccgcagcg cctcggcgcc cgccagccgg cgaagtgcct ccagatcgac cgtgtagtgg
      181 gcgggcagga cggcctgcac cggctgactg tcggcgatgc agatgacgac cttcgccgag
      241 cgccgcccgg ggatgtgggc gacggcagac agttccgggg cggtgaaggc cgggggatgg
      301 cgcagcgtga ggtagggtac gcgctcgcgc ttcagcacct cgacgaggca cgggagtaac
      361 gccactgctg ccccctttcg acgtggaaat atccggggac cgggcggttt tcatagcaca
      421 agtgttccct gtgcgtccac acttctttcg gctagaatcg gggcgtcagt tcggccgtcg
      481 cccgttgccg tcgcccggtg ggtcattggg ggagcgttaa tgaagtccag acctcgttac
      541 cccggcgtcc gcgtcaccgc taacggaaac cagctcgttt cctaccatac ggaaaccagg
      601 atcgccgacg ctggcgtctt ctacccgatc acgccgtcca cggaaggcgg cgagctctac
      661 cagcaggcct tcgccgaagg caagctgaac gcattcggcc ggaacacgat cgccatcgaa
      721 gccgaaggcg agcacgcggc gcagggtgga gcgatcgctc actctgtctg cgggaaacga
      781 gtcgtcaact acacgtcggg acagggggtc gtctacggcg tcgagcagta ctaccacgcc
      841 cccggcaagt gctcgacgat ggtgctggag gtggcagcgc gagccctcac caagcacgcg
      901 ctcaacgtgc actgcggaca cgacgacgtg tacggcgcgc tcgacacggg ctggatcatg
      961 gtcttcggaa aggacgccca gcaggcggcc gaccaggcgc tcatcctcag gcgcgtcacc
     1021 gagctcagcc tgacccccgg catgaacgtc atggacgggt tcctcacgtc gcacctcgag
     1081 cggacgttct acaagcacga atccgagttg atccgtgaat acctgggcgc tccggaagac
     1141 atcatcgact gcccgaccga aagccagcgc atcctcttcg gcccgacgcg ccgccgggtg
     1201 ccgaagatga tcgacctgac gaaccccgtg ctcctgggcc cggtccagaa ccaggagcat
     1261 tacatgcagg gcgtcgtcgc gcgccgggac aacttcacag agccgattct gcagttcttc
     1321 gaagccgcgt acgaggattt cgcgaagctc acgggccgct actacggtct ggtcagcgag
     1381 tacaagaccg aggacaccga taccgtgttc gtctcgctcg gctcggctgc ggagaacgtc
     1441 gaggcggcgg tcgactacct gcgggagcgg cgcggcgtga cagtggggtc gattcacatc
     1501 aacgtcttcc gtccgttccc ggacgcggcc gtggtgaagg cgctgaccgg caagaagaac
     1561 gtcatcgtgc tggagcggac cgacgaagcc atggcgggcg acaaccccat ggggcgcgac
     1621 attcgaacgg cgctccacaa ggcgctgcag ggcgaggcag gcctgccgaa gatcacggcc
     1681 gatcagatgc cccgcctctt cggcggcgtc tacggtctcg gctcccgcga cttcaggccc
     1741 gagcacacga tcggcgcgta tgaattcgcg accggccagc gcgcccggaa ggatggcaag
     1801 cgtgcctctg atggcgcgtc gttcatggtg ctcggcgtcg atcatccgta cgaggtgaag
     1861 tcggacgaca cgccgtcgct gctgcccgac aaggccatcg cggtccggtt ccactcggtc
     1921 ggcggctggg gcgccattac gaccggcaag aacctcggcg ccatcatcgg cgacctcaac
     1981 gatctgctgt acgagcgcga caagatcgtg gacgagttcg gcaacccgaa ggagatcatc
     2041 cacgtcagcg cgaacccgaa gtacggatcg gagaagaagg gcgcgccgac ctcgtacttc
     2101 atgatcgccg cccccgaacg catccgggtc aactgcgatc tgcgccacgt gaacgtcgtc
     2161 ctctgctgcg atcccaaggc cttcacccat accaacccgc tcgacggcat gtccgacggc
     2221 ggatgcctgg tctgggaatc agacgaggaa ggcgaggccg cgtgggagcg gctgccgctg
     2281 tgggcgcgga agcagatcat cgacaagcac atccgcgtct tcacgctgcc tggcttcaag
     2341 atcgcccgcg aggcaaccga ccggggcgac ctgcagctcc gcatgcaggg caacgcgttc
     2401 ctgggcgcct tcttttcggt ctcgccgatg ctgcaggact tccgcatcac gcccgagcag
     2461 ttccgcgacg ccgtccacaa gcagtacgtg aagaagttcg gcaagctcgg cgaaggggtc
     2521 gtgaactcga acatggaggt catgaccaag ggcttcgagc tggtacgcga gatcaacgtc
     2581 ggcgcgcttg aagcgcccga ccgctcgacg ctccgcggcc ggccgctgct cccggtgatg
     2641 atgcccgaga gcctcgccgc cagcgaaggc tgcggcacgg gctgccggac gattccgttg
     2701 ccggatgggc agaacgagcg cacgccgctc acgcgcgtgg gcgagttcga tcggatgttc
     2761 cgcgcgaagc tcgggtacaa ccagccggcc aacgcctacg cgtcgctcgg catcatggcc
     2821 gccggcagcg gcgacaccgc gtcgaagtac gtggcgcggc gcgaaacgcc gctctacatc
     2881 cccgagaact gcacgcagtg catggagtgc atcgccgtgt gcccggacac ggcgctgccg
     2941 aactgctcgc aggatctcga cacgattctg tacaccgcgg tga
//
LOCUS       DBNGSH010000385         2866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_713914, whole genome shotgun sequence.
ACCESSION   DBNGSH010000385 DBNGSH010000000
VERSION     DBNGSH010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_713914"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..2512
                     /locus_tag="AC16CY_08885"
     CDS             341..2512
                     /locus_tag="AC16CY_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353942.1"
                     /translation="MKRPGPKRRRPPQFKTKYPAGKPLGRIQYFQTQRGLTVARVDSG
                     RSPAPSRSAGPRLYAAAHRVKQRLTPRFAATVSLPVWRALGPNLIPRGQTYGATRPPV
                     SGRAVGIVISRTNPKHLVLCSGGGGLWATKDLGATWQPLTDEQPTLSMGAIAAAPSAP
                     NIVYAGTGEGDNESQLGVGLLRSSDGGQNWECIPAAALTGTGVYDIAVDPGDPLRVWV
                     GTMGALLESVNGGAAWRVVQRAQTWDISINRENPREIFAATKAGLIRSVNGGASWSRV
                     ALPGVASSAEFDRLEVCHAPSRPGIVYAAGAVDGRGVFWRRATTDGSFTAETIPPLDE
                     DSDLSQAWYDWCLAVSPADPNLLFWGATELFRGMRTSSGWTWTNVSSRRSGDSIHPDH
                     HHIAFDPSDPDVVYVCNDGGLFRSPNRGRNWTSLNRGLGITEFEFVIHMENSDDWLMG
                     GTQDNGTLSSESGGQWKQIAQGDGGDCGADEERNLCYHSYYEMDIERAAATGAGAFRW
                     RDVTPEVPDGYSCLFYPPMDVSGPVVAKAGVTLFVSDDSALHWTEVRFGTGGDNASAV
                     VIDSPAVIFVGTVSGKLFRIRRAAGGWSQATVTRLNTPRTGYISDVVPLSASVIWISY
                     SAFGRGHVFRSTDGGARWTNRSGNMPDIPINAIVVDPADARRLFAASDHGVYETRNSG
                     TRWTDFSNGLPNVVVGDLIFHERRRALIAGTRSRGAWEVTI"
     gene            complement(2522..>2866)
                     /locus_tag="AC16CY_08890"
     CDS             complement(2522..>2866)
                     /locus_tag="AC16CY_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3353943.1"
                     /translation="AGMRDWVVVYQSRSGRPEDPWLEPDICDYLREERAAGLKAAVVC
                     PIGFVCDHIEVLYDLDRQAADVSREIGLLMTRAESVNDDPLFLDMMADVVSATIRRYA
                     RGRPLPIVRAGV"
BASE COUNT          478 a          950 c          998 g          440 t
ORIGIN      
        1 caagttccac agcttcacgc tggagtgcct cgcgttcctc gccacctggc tcgtcttcga
       61 cgtggtggcc gagtgggtcg ctggaaagcc ggtcagcaca ggccgcgcag tcgagcgcac
      121 ctgattcgcc gctcgcaggg aaggcgccga tgaaacgacc gtcgaagccc ggggacaaga
      181 aagagtccgt tccgcccgga ggccgcgcgg cgtcgcgtct gcggcagcac cgcctcgcgc
      241 gtggcctgga ttcaccgcca aggccgatga ctctcaatct tgatccggat gccgcgccaa
      301 acgccgacag cgacgtgaag ccgacgagga ggaaagggcg atgaagcggc cagggcccaa
      361 gcgccgacga ccgccgcagt tcaagacgaa gtatccggcc ggcaagccgc tcggacgcat
      421 tcagtacttc cagacgcagc gcggcctgac cgtggctcgc gtcgattcgg gccgctcgcc
      481 ggcgccgtcc cgcagcgccg ggccgcgcct gtacgccgcg gcgcacaggg tcaagcagcg
      541 cctgacgccc aggttcgccg ccaccgtgtc gcttccggtg tggcgcgcgc tcggcccgaa
      601 cctcattccg cgaggccaga cctacggcgc cacgcggccg cccgtgtcgg gacgcgccgt
      661 cggcatcgtg atctcgagga cgaacccgaa gcacctcgtg ctctgttccg gcggtggcgg
      721 gctgtgggcg accaaagatc tgggcgccac gtggcagccg ctcaccgacg agcagccgac
      781 gctgtcgatg ggcgccatcg ccgccgcgcc gagcgcaccc aacatcgtct atgcggggac
      841 gggggaaggc gacaacgaga gtcaactcgg cgtcggcctc ttgcgttcgt ccgatggggg
      901 acagaactgg gagtgtattc ccgccgccgc cctgacggga acgggcgtgt acgacatcgc
      961 cgtcgatccc ggcgatccgc ttcgcgtgtg ggtgggaacg atgggcgcgc tcctcgagag
     1021 cgtcaacggc ggcgcggcgt ggagggtcgt gcagcgcgcg cagacgtggg acatctcgat
     1081 caaccgggag aacccccggg agatcttcgc cgcgacgaag gcaggactga tccggtcggt
     1141 caacggcggc gcgagctggt cgcgcgtggc gcttccaggc gtggcatcgt cggccgagtt
     1201 cgatcggctc gaagtctgtc acgcgccgtc gcgccccggc attgtctacg cggctggcgc
     1261 cgtcgatggg cgcggcgtgt tctggcggcg ggccacgacg gatggatcgt tcacggcgga
     1321 gacgatcccg ccccttgacg aggattcgga tctcagccag gcctggtacg actggtgcct
     1381 cgcggtgtcg ccagcggacc cgaacctgct gttctggggg gcaacggagc tgttccgcgg
     1441 catgcggacc tcgagcggat ggacctggac caacgtgtcg tcgaggcgca gtggcgattc
     1501 gatccatccc gatcatcacc acatcgcgtt cgatccatcg gatccggacg tcgtgtacgt
     1561 gtgcaacgac ggcggcttgt tcagatcgcc gaaccgtgga cgcaactgga cgtcgctcaa
     1621 tcgtggcctt ggcatcaccg agttcgagtt cgtgattcac atggagaact cggacgactg
     1681 gctcatgggc ggaacccagg acaacggaac cctgagcagc gagtccggcg gccaatggaa
     1741 gcagatcgct cagggcgacg gcggcgattg cggggccgac gaggagagga acctctgcta
     1801 ccactcgtac tacgagatgg acatcgagcg cgcggcggcg acgggggccg gcgcgttcag
     1861 atggagagac gtgacgcccg aggtccccga cggctactcg tgcctcttct atcccccgat
     1921 ggacgtgagc ggtcctgtcg tcgccaaggc cggcgtgacg cttttcgtgt cggatgacag
     1981 cgcgctccac tggacggagg tgcgttttgg caccggcggc gacaacgcgt ccgccgtcgt
     2041 catcgacagc ccggctgtca tcttcgtggg gaccgtgagc ggcaagctct ttcgaatccg
     2101 gcgcgctgcg ggcggctggt cacaggcaac cgtcacgcgc ctcaacacgc cgaggaccgg
     2161 gtacatcagc gacgtcgtgc cgctgtcggc gagcgtcatc tggatcagct attcggcgtt
     2221 cggccggggc cacgtgttca gatcgacaga cggcggcgcc cgctggacga accggagcgg
     2281 caacatgccg gacattccga tcaacgcgat cgtggttgac cccgcggacg ctcgacgcct
     2341 gttcgcggcg tcggatcacg gagtgtacga gacgcggaac tcgggcacgc gatggaccga
     2401 cttcagcaac ggcctgccga acgtcgtcgt cggcgacctg atcttccacg aacggcgccg
     2461 cgcgctgatt gccgggacgc gcagccgcgg cgcgtgggaa gtcacgatct gatcgttggg
     2521 gtcagacccc agcgcgtaca atcggcagcg gacggccgcg cgcgtagcgc cggatcgtcg
     2581 ccgacacgac gtcggccatc atgtcgagga agagcggatc gtcgttgacc gactcggcgc
     2641 gcgtcatgag gagaccgatc tcgcggctga catcggcggc ctgacgatcc aggtcgtaca
     2701 gaacctcgat gtggtcgcac acgaacccga tcgggcacac gacggccgct ttcagaccgg
     2761 cggcgcgttc ctcccggaga tagtcgcaga tgtcgggctc gagccacggg tcttccggcc
     2821 tgccgctccg gctctggtac acgaccaccc aatcgcgcat gccggc
//
LOCUS       DBNGSH010000386         3715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_373929, whole genome shotgun sequence.
ACCESSION   DBNGSH010000386 DBNGSH010000000
VERSION     DBNGSH010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_373929"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="AC16CY_08895"
     CDS             complement(<1..1323)
                     /locus_tag="AC16CY_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3353944.1"
                     /translation="MLQARTIRCSVAAVLFLAICGLSAPAGAQYFGSNKVRYKNFDFQ
                     VLRTEHFDIYFYPSEKAGVDIAARLAERWYVRLERLLQHRLRGRQPLILYGSHVDFEQ
                     TNVIGGEIGESTGGVTESLRRRIVLPLAGPLADTDHVIGHELVHAFQFDITTTSEMEA
                     GETGAHRLPLWFIEGMAEYLSIGPVDSNTAMWLRDATRREKLPTVKDLDNPEYFPYRW
                     GQAFWAYVGGRWGDEVIARMLVLAAAQGVEPALEQVLGIDAKQFSADWHAAILKAYTP
                     VLTAPTRTTELGRLVIEGEQMLANVNVAPSVSPDGKWLAYVSTRGFFSVDVYIAEVAT
                     GRVVKRLTSTATNAHYSSIQFIYSAGAWDRASRQIAVATVTSGKAALAVFDAERGSLS
                     REIEVPGVDEIMNPTWAPDGSAIAFTGLHQGLTDLFVYDLNAGTLKQLT"
     gene            1523..>3715
                     /locus_tag="AC16CY_08900"
     CDS             1523..>3715
                     /locus_tag="AC16CY_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019223.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HIR3353945.1"
                     /translation="MRRFCALVLVVLAGWTLAFAAAEPRAFSERQRNWWSLKPIVRPD
                     PPAVKARNWVATPVDAFVLAKLEAAGIAPNPRADRRTLLRRATIDLTGLLPTEQEIQQ
                     FQSDTSPDAWPKAVDRLLASPAYGERWGRHWLDVARYADSNGFKADETRPHIWRYRDY
                     VVKSFNDDKPYDQFVREQIAGDELFPGNPEALVAMGFNRHWIDETNAAGLLLRRQETI
                     DEMTNATGAVFLGMTVACARCHDHKFDPILQKDYYRLQAFFANTVAGDGPLPLSDAAE
                     RRAYDAKKAEWDAKTADIRAEMNAILEPIRISRAQSGSNTFEDEVKAAIFMEPAKRDP
                     FQAVMAKTAEPRVDLADDPDARTLRTLKPDAAARYAELKKQLASFDAIKPGQLDEGQY
                     MVDLSATAPPTYLLKGGNLQARQEEVKPGFLTILDPGDAAITPPAGLNSTGRRSALAA
                     WLTDVKNPLPARVMVNRVWHYHFGRGLAATPGDLGLMGSRPTHPELLDYLASTFQRGW
                     SIKALHRTIMLSNTYQQSSATTAASAKADPDNKLLWRYQRRRMEAEAVRDTMLQVSGL
                     LNSKMGGPGVFPPLPPGTISELSATAAAGGWRTETDPAQSNRRSVYVFVRRNLRYPML
                     QDFDVANTFESHAARTNTVTASQSLDLMNNDLMLDWARGLAGRVVKDAGPDPLAQVDR
                     AFRLAYGRRATQEEQELVTEFLERQMPILAERFAKGGKSTPALPMDLPD"
BASE COUNT          652 a         1256 c         1229 g          578 t
ORIGIN      
        1 ggtcagctgt ttaagcgtcc cggcgttcag gtcgtacacg aacagatcgg tcaacccctg
       61 atgcagtccc gtaaaggcga tggcgctgcc gtcgggcgcc cacgtcggat tcatgatttc
      121 atcgacgccc ggcacctcga tctcgcggct gagcgacccg cgctccgcgt cgaacacggc
      181 aagcgccgcc ttgcccgacg tgaccgtcgc gacagcgatc tgccggctgg ccctgtccca
      241 tgcgcccgcc gagtagatga actgaatgct cgagtagtgg gcgttggtgg cggtgctcgt
      301 cagccgcttg acgacacggc cggtcgcgac ctccgcgatg tagacgtcaa cggagaagaa
      361 gccgcgcgtg gacacgtagg ccagccactt cccgtcgggg ctgacggagg gggcgacgtt
      421 gacgttggcc agcatctgct cgccttcgat gaccagccgc cccagttcgg tggttctcgt
      481 cggcgccgtg aggacgggcg tgtaggcctt caggatcgcg gcgtgccagt ccgcggagaa
      541 ctgtttcgcg tcgatgccga gcacctgttc gagagcgggc tcgactccct gggccgcggc
      601 cagcaccagc atccgggcga tcacctcgtc gccccaacgg ccgccgacgt aggcccagaa
      661 ggcctggccc catcgatacg gaaagtactc ggggttatcg agatctttga ccgtcggcag
      721 cttctcgcgg cgggtcgcgt cgcgaagcca catcgccgtg ttggaatcga cggggccgat
      781 cgagaggtac tcggccatgc cttcgatgaa ccacagcgga agtcgatgcg cgccggtttc
      841 accggcctcc atctcggagg tcgtcgtgat gtcgaactga aacgcgtgaa ccagctcgtg
      901 gccgatcacg tggtcggtgt cggcaagcgg cccggccagc ggcagcacga tgcgccgccg
      961 gagcgattcg gtgacgccgc ccgtgctctc gccgatctcg ccgccgatca cgttggtctg
     1021 ttcaaagtcg acatgcgagc cgtacagaat cagcggctgg cggccgcgga gacggtgctg
     1081 gagcaggcgc tccagacgga cgtaccagcg ttcggccaac cgggcggcga tatccacccc
     1141 ggccttctcg ctcggataga agtagatgtc gaagtgctcg gtcctgagca cctggaaatc
     1201 aaagttcttg tagcggacct tgttgctgcc gaagtactgg gccccggcgg gagcgctcag
     1261 gccgcagatg gcgaggaata gcacagcggc gacggagcaa cgaatcgttc gggcctgtaa
     1321 catccaacac ccctccgcgg cgccttgctg caagccgcgc accagtgtga tcaggacgat
     1381 cctcgcgatt caaacgcggt tcgcgcccgg gacctgaccg gtattcagaa cagatcacta
     1441 tcttacgtct cccggggacg cccgaaaggc ggtctggccc ggccgtaata cctgttaaga
     1501 tctcggcacc tcgggaggca gcatgcgtcg attctgcgcc ctcgtcctgg tggtgctggc
     1561 cgggtggacc ttggcgtttg cggcagccga gccgcgcgcc ttctccgaac gacaacgcaa
     1621 ctggtggtcg ctcaagccga tcgtccggcc cgacccgccg gccgtcaagg cccgcaactg
     1681 ggtggcgaca cccgttgacg ccttcgtgct cgcgaaactg gaggcggcgg gcattgcccc
     1741 gaacccgcgc gccgaccggc gcacgctcct gcgccgtgcc acgatcgacc tgaccgggct
     1801 gctgccgacc gagcaggaga tccagcagtt ccagtccgac acctcgccag acgcgtggcc
     1861 gaaggccgtc gatcgcctcc tcgcctctcc ggcatacggg gagcgctggg gacggcactg
     1921 gctcgacgtc gcccgctacg cagacagcaa cggcttcaag gccgacgaga cgcgccccca
     1981 catctggcgc taccgcgatt acgtcgtgaa gtcgttcaac gacgacaagc cgtacgacca
     2041 gttcgttcgc gagcagatcg ccggcgacga gctctttccg ggcaacccgg aggcgctcgt
     2101 cgcgatgggc ttcaaccgcc actggatcga cgagacgaac gcggccggcc tgctgctccg
     2161 tcgtcaggaa acaatcgacg agatgaccaa cgccacgggc gcggtcttcc tggggatgac
     2221 cgtcgcgtgc gcgcgctgcc acgaccacaa gttcgatccg attctgcaga aggactacta
     2281 ccgcctgcag gcgttcttcg ccaacacggt cgccggcgac ggtccgctgc cgctcagcga
     2341 cgcggccgag cgccgcgcgt acgacgcgaa gaaggccgag tgggacgcca agacggcgga
     2401 tatccgggcc gagatgaatg cgatcctcga gcccattcgc atctccaggg cgcagtcggg
     2461 gtcgaacacg ttcgaagacg aagtcaaggc cgcgatcttc atggagccgg ccaaacgcga
     2521 tccgttccag gctgtgatgg ccaagaccgc ggaaccgcgc gtcgatctcg ccgacgatcc
     2581 cgacgcccgg acactgcgca cgctcaagcc ggacgcggcg gcccgctacg cggagttgaa
     2641 gaaacagctc gcctcgttcg acgcgatcaa gccgggacag ctcgacgaag gccagtacat
     2701 ggtggacttg agcgccactg ccccgccgac ctaccttctc aagggcggca acctgcaggc
     2761 gaggcaggaa gaggtgaagc ccgggttcct gacgattctc gacccgggtg atgcggcgat
     2821 cacgccgccc gccgggctga actccacggg ccgccgcagc gcgctggccg cgtggctgac
     2881 cgacgtgaag aacccgctgc cggcgcgcgt catggtgaac cgcgtctggc actaccactt
     2941 cggccgcggc ctcgcggcga cgccgggcga tctgggcctc atgggctcgc ggcccacgca
     3001 tccggagctg ctcgactacc tcgcctcgac cttccagcgc ggctggagca tcaaggcgct
     3061 gcaccggacg atcatgttgt cgaacacgta ccagcagtcg tcggcgacga cggcggcttc
     3121 ggccaaggcg gatccggaca acaagctgct ctggcggtat cagcggcgca ggatggaagc
     3181 agaggcggtc cgcgacacga tgctccaggt gtctgggctg ctcaactcga agatgggcgg
     3241 gccgggcgtc ttcccgcccc tgcctccggg gacaatcagc gagctgtcgg caacggcggc
     3301 cgcgggcggg tggcgcactg aaaccgatcc cgcgcagtcg aaccggcgca gcgtatacgt
     3361 cttcgtgcgg cgcaacctgc ggtatccgat gctgcaggat ttcgacgtgg cgaacacctt
     3421 cgagagccac gccgcgcgga ccaacacggt caccgcgtct cagtcgctcg atctgatgaa
     3481 caacgacctg atgctcgact gggcccgcgg gctggccggg cgcgtggtga aggatgccgg
     3541 accggatccg ctggcacagg tcgatcgcgc gttccgactg gcttacggcc gccgcgcgac
     3601 gcaggaggag caggagctgg tcaccgagtt cctcgagcgc cagatgccga tcctcgccga
     3661 gcggtttgcg aaaggcggca agtccacgcc cgcattgccc atggatctgc cggac
//
LOCUS       DBNGSH010000387         1545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4619457, whole genome shotgun sequence.
ACCESSION   DBNGSH010000387 DBNGSH010000000
VERSION     DBNGSH010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4619457"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="AC16CY_08905"
     CDS             <1..892
                     /locus_tag="AC16CY_08905"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3353946.1"
                     /translation="PTAGKTRERARTELVTLLGLAAARGARGVAFDYTFDGESAVDLL
                     LCTTVESIGIPVFFGYGFERFEQVLNPFPFPRSLQSCAKPDRQGHLVGFLDADRRARV
                     TPLFFNNDRQRPALSLAVARALSGESEISIPDNGLLHFIEPAQAHLRVRLEDLLDESA
                     SRESARNALNDRFVLVGEESAADSFDTPFGHKPGVIVHADAIHSLAHSHYIRKGPWWL
                     RFFPMLAFCYGLAVWSAHDGPAGRLALVCLVATACFCVTAAVGIVAGPLWFDAFYPAA
                     AVWLLLPLLLSLRRLTRARR"
     gene            complement(911..>1545)
                     /locus_tag="AC16CY_08910"
     CDS             complement(911..>1545)
                     /locus_tag="AC16CY_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR3353947.1"
                     /translation="LTETAFGVVYSRVSDGAGSFTFPGLPPGRYRLVAVLPGFATLST
                     EVTLAAGENQQSRLAMRVGSLVETITVRCETVGGALRPGARGVMARGMPEVTPLFTLS
                     QAAPAAQDRPVTRVQPIRVGGQIRAPRKVKDALPVCPAASLPASGALVILEAIVGADG
                     LVGDVKVLRSSPPFDQPSIDAVRQWEFTPTLLNNAAVPVVMTVTVSYQRG"
BASE COUNT          229 a          536 c          500 g          280 t
ORIGIN      
        1 gcctacggcg ggcaagacgc gcgagcgcgc tcgaaccgag ctcgtcaccc tgttggggct
       61 cgctgcggca cgtggcgcgc ggggtgtcgc attcgactac accttcgacg gtgagtcggc
      121 tgtcgacctg ctcctgtgca ccaccgtgga aagcatcggg ataccggtct tcttcgggta
      181 cgggttcgag cggttcgagc aggtgctgaa cccgttcccc tttccgcgca gccttcagtc
      241 ctgtgcgaag ccggatcgtc agggccacct cgtgggcttc ctcgatgccg accgccgcgc
      301 tcgcgtcacg ccgctcttct tcaacaacga ccgccagcgg ccggcgctca gtctcgccgt
      361 ggctcgcgcg ctatccggcg agtcggagat ctcgatcccc gacaatgggc tccttcactt
      421 catagagccg gcgcaggcgc acctccgtgt acgactcgaa gatctgctcg acgaatcggc
      481 gagcagggag tccgcgcgca atgccttgaa cgaccgcttc gtgctggtgg gtgaagagtc
      541 ggccgccgat tcgttcgata cgcctttcgg acacaagccc ggcgtcatcg tgcacgccga
      601 cgccatccac tccctcgcgc actctcacta catacggaaa ggcccgtggt ggctccgatt
      661 cttccccatg ctggcgttct gctatggact cgccgtgtgg tccgcgcacg acgggccggc
      721 aggccgactg gcgcttgtgt gcctggttgc gaccgcctgc ttctgtgtga ccgccgctgt
      781 cggcatcgtg gcgggcccgt tgtggttcga tgcgttctac ccagcagcgg ccgtctggct
      841 tctgctgccc ctgttgttgt ctctccgtcg tctcactcgc gcgcgccgct gaaggcgagc
      901 gggcttcggc ttacccacgc tggtaggaca cggtgactgt catgacgacc ggcaccgccg
      961 cgttgttcag cagcgtcggc gtgaactccc actgccgcac cgcgtcgatc gacggctggt
     1021 cgaacggggg gctcgagcga agtactttga cgtctcctac caggccgtcg gcgccgacga
     1081 tcgcctcgag gatgacaagg gcgcccgagg ctggcagcga agccgccggg cacaccggga
     1141 gcgcgtcttt caccttccgg ggcgccctga tctgaccgcc gacgcggatg ggctgaacac
     1201 gcgtcacggg ccgatcctgc gccgcggggg cagcctgcga cagcgtgaag agcggcgtca
     1261 cctccggcat cccgcgcgcc atgactccgc gtgcgcccgg gcgcagcgcc ccgccgacgg
     1321 tctcacagcg aacggtgatc gtttcgacga gcgatccaac gcgcatcgcg agcctgctct
     1381 gctggttttc gccggccgcg agcgtcactt ctgtcgacag ggttgcaaag ccaggcagta
     1441 cggcgacgag ccggtaacgt cccggcggca gaccagggaa cgtaaacgag cctgctccgt
     1501 cgctcacccg tgagtacacg acaccaaagg cagtttcggt cagtg
//
LOCUS       DBNGSH010000388         1903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3430241, whole genome shotgun sequence.
ACCESSION   DBNGSH010000388 DBNGSH010000000
VERSION     DBNGSH010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3430241"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="AC16CY_08915"
     CDS             <1..1707
                     /locus_tag="AC16CY_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR3353948.1"
                     /translation="VVHPEGQRRAHGVVNTLWILASVAVFAWPLLDIDAFVNRAATPG
                     AIDLAAGCLALVIVLEATRRTTGWVLPITAILFLVYAMTGPLLDRIGLSLVAHRGYGV
                     ERLVGTLFMTLEGIFGVPLDVATTYIVLFTIYGAVLEHSGAGRFFVDWTSAAVGRSGG
                     AGPGRTVTVAGYLLGAVSGSGVANTVMLGSITWPVMRRAGYPPDAAGAVLAAAGIGAI
                     LAPPVMGAAAFLIAEFLNISYLQVIITAAIPAALYYLSVFLMVEADSHRLSLQPIDSE
                     SAPLGALTKRGWYHFASMISIVVLLTQGFTTFRAVFFATLLAVALSFLDRGTALGPRR
                     LAGALASGGRSVLPVAATTATAGIIVGVITLTGLGLKVAGLIVSLAGGSLPLTIVYSG
                     IAVWMLGLAVPVTASYIIAAVMVVPALVQVGVAEIAAHMFVFYYAVLSEVSPPTALSP
                     FAAAAITGGQPVRTTMLSWKYTAPAFIVPIAFTIDPQGLGLLLQTAPGDAVWATVKAV
                     VGITALAAAASGWIGRAATPVQRGLAGAAAAMLFAPGVAPAFAGASLFALVVAIRRRS
                     QS"
BASE COUNT          234 a          649 c          658 g          362 t
ORIGIN      
        1 gtcgttcacc ccgagggcca gcgccgggcg catggcgtcg tcaacacgct ctggattctc
       61 gcgtcggttg ccgtcttcgc gtggccgctc ctggacatcg acgcgttcgt caatcgagcc
      121 gcgacgcccg gcgccatcga tctcgccgcg ggctgcctcg ccctcgtcat cgtgctcgag
      181 gcgacacggc ggaccacggg gtgggtgctt ccgatcaccg ccattctgtt cctcgtgtac
      241 gcgatgaccg ggccgctgct cgatcggatc ggcctgtctc tggtggcgca ccggggctac
      301 ggcgtcgaac ggttggtcgg cacgctgttc atgaccctcg aagggatctt cggcgtcccg
      361 ttggacgtgg ccacgaccta tatcgtgctg ttcaccatct acggcgccgt gctcgagcac
      421 agcggcgcgg gacgcttctt cgtggactgg acgagcgccg cggtcggccg ctcgggcgga
      481 gccggccccg gacgaacggt gacggtggcc ggctatctgc tgggggcggt gtcgggcagc
      541 ggcgttgcca atacggtgat gctcggctcg attacgtggc ccgtgatgcg gcgcgcgggc
      601 tatccgcccg acgcggcagg cgcggtcctc gccgccgcag gcatcggcgc catcctcgcg
      661 ccgccggtca tgggcgcagc ggcgtttctc atcgccgagt tcctgaacat ctcctacctc
      721 caggtcatca tcaccgcggc catccccgcc gccctgtact atctgtcggt cttcctgatg
      781 gtcgaggccg actcacatcg cttgtcgctg cagccgatcg actccgaatc cgcgccgctc
      841 ggcgccctga cgaagcgagg ctggtaccac ttcgcctcga tgatctcgat cgtcgtgctg
      901 ctgacgcagg gctttacgac atttcgcgcc gtcttttttg ccacgctcct ggcggtcgcg
      961 ctgagtttcc tggaccgcgg gacggctctc ggtccgcgtc gcctggcggg ggcgctcgcc
     1021 tccggggggc ggagcgtgct tccagtcgcc gcgacgaccg ccaccgccgg catcatcgtg
     1081 ggggtcatca cgctgaccgg cctcggcttg aaggtggccg gcctcatcgt gtccctggcc
     1141 ggagggagcc tgccgctgac catcgtgtac tcggggattg ccgtctggat gcttggcctg
     1201 gccgtgcctg tcacagcgtc ctacatcatc gcggcggtca tggtcgtgcc ggcgctcgtg
     1261 caggtcggcg tggcggagat cgccgcgcac atgttcgtgt tctattacgc cgtgctgtcc
     1321 gaggtgagcc cgccgaccgc gctctcgccg tttgccgcgg cggccatcac cggggggcag
     1381 ccggtgcgca cgacgatgct gtcatggaag tacacggcgc cggccttcat cgttccgatt
     1441 gccttcacga tcgacccgca gggactgggc cttctgctgc agacggcgcc cggtgacgcc
     1501 gtgtgggcca cggtgaaggc cgtcgtgggc atcaccgcgc ttgcggccgc cgccagtgga
     1561 tggattggcc gcgcggccac accggtgcaa cgaggcttgg cgggagcggc tgccgcgatg
     1621 ctgttcgcgc cgggtgtcgc gccggctttc gcaggagcgt cgctgttcgc gctcgtcgtg
     1681 gccattcgtc ggcgttcgca atcgtgatcg ttcaacggga gcatcagagc atggctcgat
     1741 acacgctcgt ctacgggatc cggttggtgc cggagggaac gctcgtcggt gtggacgagg
     1801 cccggctcga attgaagggc ggtcaggaag cggggctgac cctgcacacg atcgacggca
     1861 cgatcccgca actgcgccgc gcgctcgacc gaagcctcga tgc
//
LOCUS       DBNGSH010000389         3030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2921507, whole genome shotgun sequence.
ACCESSION   DBNGSH010000389 DBNGSH010000000
VERSION     DBNGSH010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2921507"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC16CY_08920"
     CDS             <1..357
                     /locus_tag="AC16CY_08920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3353949.1"
                     /translation="AAAWSPNPKNPPDFFDMPVKDGEPQPLVIAKWAANAPLAMIDQY
                     VTNLKKYKAITIEVGTQDGLAASNRQMDQILTDFGVPHSFETYEGDHTNRVVERIETK
                     VLPFFSSNLSFTAPRR"
     gene            complement(374..1090)
                     /locus_tag="AC16CY_08925"
     CDS             complement(374..1090)
                     /locus_tag="AC16CY_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3353950.1"
                     /translation="MSRFTQWVTFDCFGTLVDWQTGLASFLAPYAGDQAGSIVRAYHA
                     HERILERGYRPYAEVLATGLTRAAAEHGVEPVPQDASALRRAWASMRLFDDVEPMLAE
                     LRSRGYGLAVLTNCDDDLFEITHRSFKAPFDLFLTSERVRGYKPAPWHFRGFERLTGA
                     SRTGWVHVACSWYHDIEPARRLGIWRVWLDRERTGEDPSSASAVVHAAGDVVPAVARL
                     LETPRGGVPPRREVASLAGV"
     gene            complement(1201..1356)
                     /locus_tag="AC16CY_08930"
     CDS             complement(1201..1356)
                     /locus_tag="AC16CY_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353951.1"
                     /translation="MSGTSSERPVFPNAVHATFAHSVTALLVSPAVVRITKAGRLDWL
                     VPPGHSG"
     gene            1416..1979
                     /locus_tag="AC16CY_08935"
     CDS             1416..1979
                     /locus_tag="AC16CY_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3353952.1"
                     /translation="MQASDDAAVADARSGDREAFRTLVERHSRYLYGVAYRMTGNAED
                     ARDVVQDTWLKAHSQLGRFEARANVRTWLTRIAINCAIDYLRRTRRHEAPGDTSADGR
                     SWHEQPTLQPLPDRLVLAGEIGTRIEGTMRCLTTLERAAFVLRHVEGMSIDEIGNALG
                     LNTSATKHSIFRAVRKMRVALAPFKEA"
     gene            1981..2739
                     /locus_tag="AC16CY_08940"
     CDS             1981..2739
                     /locus_tag="AC16CY_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3353953.1"
                     /translation="MSSGEGRHLSDEDLLLAYYGESTPSDRARVEAHVSECEQCRALD
                     RELRDVLAAVETTPLAEPPTGFEREMWARIEPLLPVPRAQRPRWNLALPRWAFAASVA
                     VLVVGAFALGRVWDRPPEESSAPIAIDWASADRVLRAEVEDHFDRSERVLVELVNADY
                     TISDGVGDRARAADLVSAGRLYRRSAQNIGDAEMGSLLEDLERVLVDVANGPVDREPD
                     EIARLRERIDEQDLIFRLRVVKEEMRVTDGERVR"
     gene            2736..>3030
                     /locus_tag="AC16CY_08945"
     CDS             2736..>3030
                     /locus_tag="AC16CY_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353954.1"
                     /translation="MTRRRCVAVAGMLAAAFLSVSPGLAGAEQRPGTVPRVEPPEPPE
                     PPEPPEPPEPPEPFEPLEPFEPLEPLEPLAPLFELALQQTASDAERAARNAARA"
BASE COUNT          549 a         1006 c         1056 g          419 t
ORIGIN      
        1 gccgccgcgt ggtcgccgaa tccgaagaac ccgcccgact tcttcgacat gccggtgaaa
       61 gacggcgagc cgcagccgct cgtgatcgcc aagtgggccg cgaacgcgcc gctcgcgatg
      121 atcgatcagt acgtcacgaa cctgaagaag tacaaggcga tcacgatcga ggtcggcacg
      181 caggacggcc tggccgcctc gaaccggcag atggaccaga tcctcaccga cttcggcgtg
      241 ccgcactcct tcgagaccta cgaaggcgat cacaccaacc gcgtcgtcga gagaatcgaa
      301 acgaaggtgc tcccgttctt ctcgagcaac ctgtccttca cggcgccgcg gaggtagggc
      361 tccccgccga gccctacact ccggccagcg acgcgacctc acggcgcgga ggaacccctc
      421 cgcgtggagt ctcgagcaga cgggcgaccg ccgggacaac atcaccggcg gcgtgcacga
      481 cagcagacgc cgacgaggga tcctcgccgg tgcgctcgcg atcgagccac acgcgccaga
      541 ttcccagccg gcgcgccggc tcgatgtcgt ggtaccagct gcacgccacg tgcacccacc
      601 cggtccggct ggcgcccgtc aggcgctcga acccgcggaa gtgccacggc gcgggcttgt
      661 agccgcgcac gcgctccgac gtgaggaaca ggtcgaacgg cgccttgaac gagcgatgcg
      721 tgatctcgaa cagatcgtca tcgcagttgg tcagcaccgc cagcccgtag cccctcgagc
      781 gcagctccgc cagcatcggc tccacgtcgt cgaagaggcg catggacgcc cacgcgcggc
      841 ggagcgcgga agcgtcctgc ggcaccggct cgacgccgtg ctcggcggcc gcgcgcgtca
      901 gcccggtggc gagcacctct gcgtacggtc tgtagccgcg ctcgaggatg cgctcgtggg
      961 cgtgatacgc gcgaacgatg cttcccgcct ggtcgcccgc gtacggagcc agaaacgagg
     1021 caagccccgt ctgccagtcc acgagcgtgc cgaagcagtc gaaggtgacc cattgtgtga
     1081 agcgtgacac tgcaacctcc ccggtccgcc tgaaggcgga cactacgtac caaaacaaaa
     1141 gggccatcac cctgtccgga tgatggccct cgaaactcgc gaagaaggtg tggtcttaca
     1201 tcatccggaa tgacctggcg gcaccagcca atcgagccgt cccgccttcg taatgcgaac
     1261 gacggccggg ctgacaagca gcgcggtcac cgagtgcgca aacgtggcgt gaacagcgtt
     1321 gggaaacacg ggtcgctccg aagatgtgcc cgacatgata ccgcagatcg cgccgccggc
     1381 caaccaatgg ccgcggtccc cgtctaactt gaagcatgca ggcgagcgac gacgctgccg
     1441 tcgccgacgc gagaagcggc gacagggagg cctttcgtac gctcgtggag cgccacagcc
     1501 gctatctgta cggggtggcc taccgaatga cgggcaacgc cgaggacgca cgggacgtgg
     1561 tccaggacac gtggctcaag gcgcactcgc agcttgggcg tttcgaggcg cgggcgaacg
     1621 tgcggacgtg gctgaccagg atcgccatca actgcgcgat cgactacctc cgcaggaccc
     1681 ggcggcacga ggcgccgggt gatacgtcgg ctgacgggcg cagctggcac gaacagccca
     1741 cgcttcagcc gctgccggat cgtctggtgc tggccggcga gatcgggacg cggatcgagg
     1801 gaacgatgcg gtgcctgacg acgctcgagc gcgcggcgtt cgtgctgcgg cacgtcgagg
     1861 ggatgtcgat cgacgaaatc gggaacgcgc tcggcctgaa cacgagcgcc accaagcaca
     1921 gcatcttccg ggccgtgagg aaaatgcgcg tggcgctggc gccgttcaag gaggcatgag
     1981 gtgtcatcag gcgagggccg ccatctgagc gacgaggatc tgctgttggc ctactacggc
     2041 gaaagcacgc cgtccgaccg cgcgcgcgtc gaggctcacg tgtcggaatg cgagcagtgc
     2101 cgcgcactcg atcgcgaact gcgcgacgtg ctcgcggccg tcgaaacaac gccgctcgcg
     2161 gagccgccga ccgggttcga gcgcgagatg tgggcgagga tcgagccgct cctgccggta
     2221 ccgcgcgcgc agcggccgcg gtggaatctc gcgctgccgc gctgggcgtt cgcggcaagc
     2281 gtcgcggtgc tcgtcgtcgg cgccttcgcg ctgggtcgtg tgtgggatcg cccgccggaa
     2341 gagtcgtccg cgcccatcgc gattgactgg gcgagcgccg atcgggtgct ccgggcggag
     2401 gtcgaggatc acttcgaccg ttcggagcgg gtcctcgtcg agctggtcaa cgcggactac
     2461 acgatcagcg acggtgtcgg cgaccgggca cgtgcggcgg acctcgtctc ggcgggccgg
     2521 ctgtatcgcc gatcggctca gaacatcggc gacgcggaga tgggcagcct cctggaagac
     2581 ctggagcgtg tgctcgtgga cgtcgccaac ggccctgttg accgggagcc ggacgagatt
     2641 gcgcgtctgc gcgagcgcat cgacgagcag gacctgatct tcaggctgcg cgtcgtgaag
     2701 gaagagatga gagtcacaga cggtgagagg gtgagatgac aagacgacga tgcgtggcgg
     2761 tggcggggat gctggcagcg gctttcctga gtgtgagccc cgggcttgcg ggggcggagc
     2821 agaggcccgg aaccgtgccg cgggtggagc caccggaacc accggaacca ccggagccgc
     2881 ccgaaccgcc cgaaccgccc gaaccatttg aaccgcttga accgtttgag ccgcttgaac
     2941 cactcgagcc gctcgcgcca ctgttcgagc tggcgcttca gcaaaccgct tcggacgcag
     3001 agcgggcggc acgcaacgct gcgcgcgccg
//
LOCUS       DBNGSH010000390         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4449374, whole genome shotgun sequence.
ACCESSION   DBNGSH010000390 DBNGSH010000000
VERSION     DBNGSH010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4449374"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="AC16CY_08950"
     CDS             <1..623
                     /locus_tag="AC16CY_08950"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3353955.1"
                     /translation="GKLLIDMSTVGPAASRALAAKVREKGADMLDAPVSGSTSMVEQG
                     KLSIMAGGHRATFDSAKPLLEDIGQKVTYIGENGLALSMKLATNISIAVQMLALAESV
                     VLAEKAGVARETAIEVLTNSAIASPLVQYKGPCVLKMPEQPMFSANMMQKDVTLALEL
                     AREVQVTLPTGAVVNEFLSSARAQGLGEQDFAILYKVLAQLSGVKA"
     gene            620..1585
                     /locus_tag="AC16CY_08955"
     CDS             620..1585
                     /locus_tag="AC16CY_08955"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015494797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3353956.1"
                     /translation="MSIPQEKLLRLYRQMVAIRLFEERVNDLYTRAVMPGLAHLYIGE
                     EAIAVGVCEALRSDDYITSTHRGHGHCLAKGAKPHLMFAELLGKEAGYCKGKGGSMHI
                     ADPATGNLGANAIVGGSAGIATGAAYASKYLKQGRVAVCFFGEGALGQGLLYEELNLS
                     QLWKLPVIFVCENNGYNEYTHFSESTAGDVSARAAAFGMANETADGQNVREVYGVASK
                     YIERARRGEGPAFLQFKTYRYHGHHVGDVARAYYRPKAEEQEWVTQRDPITLHGQWLM
                     DNGIAARPALDAIQEELIKEMDAAVEFAVSAPYPDPSKVNQDVYV"
     gene            1578..2597
                     /locus_tag="AC16CY_08960"
     CDS             1578..2597
                     /locus_tag="AC16CY_08960"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3353957.1"
                     /translation="MSETAAPPLQATGVRELTFGEAIKEAIAEEMRRDPKVVLMGEDV
                     AEAGTTFKVLLGLVDEFGKDRIIDTPISEAGFTGIGVGAAMTGMRPIVDIMFGDFLMI
                     IMDQVANQAAKIHYMSGGKWKVPLVIRATMGATRRSAAQHSQSLHAWPSHIPGLKVVV
                     PSTPYDAKGLFKAAIRDDNPVVIFEHKISYKKVKGPVPAEDYVIPLGVADVKRAGSDI
                     TLVALSSMVQTALGAAKMLEEVGINAEVVDPRTTWPLDEKTLVESVKKTSRCIVVDEG
                     YFRYGITGELASVIQEQAFYDLDGPVRRLGAMHVPIPFSPALEDVTVPSERQVFDLAR
                     QMCNR"
BASE COUNT          541 a          883 c          966 g          440 t
ORIGIN      
        1 ccggcaagct gctcatcgac atgagcacgg tcggcccagc ggccagccgc gcgctggcag
       61 cgaaggtgcg ggaaaagggc gccgacatgc tcgacgcgcc ggtctcgggc agcacctcga
      121 tggtcgaaca aggcaagctg tcgatcatgg ccggcggaca tcgcgcgacc ttcgacagcg
      181 ccaagccgct gctcgaggac atcggccaga aagtcaccta catcggcgag aacggcctcg
      241 cattgtccat gaagctcgcg accaacatca gcatcgccgt ccagatgctc gcgttggccg
      301 agagcgtcgt tctggctgag aaggccggcg tggcccgcga aacggcgatt gaggtgctga
      361 ccaacagcgc gatcgcttcg ccgctggtgc aatacaaggg accgtgcgtc ctgaagatgc
      421 ccgagcaacc gatgttcagc gcgaacatga tgcaaaagga tgtgacgctc gcgctcgagc
      481 tggcccgcga ggtgcaggtg acgttgccca ccggggccgt ggtcaacgag ttcttgtcgt
      541 ccgcacgcgc gcagggcctt ggggaacagg atttcgccat cctgtacaaa gtgctcgcgc
      601 agctctccgg agtgaaggca tgagtatccc gcaggagaag ctcctccggc tgtatcgcca
      661 gatggtggcg atccgcctgt tcgaggagcg cgtcaacgat ctctacacgc gcgcggtgat
      721 gccgggcctg gcgcacctct acatcggcga ggaggcgatc gccgtggggg tgtgcgaagc
      781 gctccggtcc gacgactaca tcacgagcac gcaccgcggc cacggacatt gcctggccaa
      841 gggcgccaag cctcatctca tgttcgcgga gctgctcggc aaggaagcgg gctactgcaa
      901 ggggaagggc gggtcgatgc acatcgccga ccccgccacc gggaacctcg gcgccaacgc
      961 catcgtcggc ggcagcgccg ggatcgcgac cggggcggcg tacgcgtcca agtacctgaa
     1021 gcaggggcgc gtggccgtct gtttcttcgg cgagggcgcg ctcggccagg ggctgctcta
     1081 cgaggagttg aacctctcgc agctctggaa gctgccggtc atcttcgtgt gcgagaacaa
     1141 cggctacaac gagtacacgc acttctcgga atccacggcg ggcgacgtca gcgcccgggc
     1201 ggctgccttc ggcatggcca acgagacggc ggacggccag aacgtccgcg aggtctacgg
     1261 cgtggcgtcg aagtacatcg aacgggcgcg ccgcggcgag ggtccggcgt tcctccagtt
     1321 caagacctat cgctaccacg gccaccatgt cggcgacgtc gctcgcgcct actaccgccc
     1381 gaaggccgaa gagcaggagt gggtcaccca gcgggacccc atcacgctgc acggccagtg
     1441 gctgatggac aacggtattg ccgcgcgccc ggcgctcgat gccattcagg aagagctgat
     1501 caaggaaatg gatgcggcag tggagttcgc cgtgagcgcg ccgtatccgg acccgagcaa
     1561 ggtgaaccag gatgtctatg tctgaaaccg cggcaccgcc ccttcaggcg accggcgtac
     1621 gggagctgac cttcggcgag gcgatcaagg aagcgatcgc cgaggagatg cgccgcgatc
     1681 cgaaggtcgt cctcatgggc gaggacgtcg ccgaagcggg gacgacgttc aaggttctgc
     1741 tgggactggt agacgagttc ggcaaggacc gcatcatcga cacgccgatc tcggaagccg
     1801 gcttcaccgg catcggcgtc ggagcggcga tgaccggcat gcgtccgatc gtggacatca
     1861 tgttcggcga cttcctgatg atcatcatgg accaggtcgc caaccaggcc gccaagatcc
     1921 actacatgtc gggcggcaag tggaaggtgc cgctcgtgat ccgggcgacc atgggcgcca
     1981 cgcgcaggtc ggcggcgcag cactctcagt cgctgcacgc gtggccgagc cacatcccgg
     2041 gcctgaaggt cgtcgtcccc tcgacgccgt acgacgcgaa ggggttgttc aaggcggcga
     2101 ttcgcgacga caacccggtc gtcatcttcg agcacaagat cagctacaag aaagtgaagg
     2161 gcccggtgcc ggcggaggat tacgtgattc cgctcggcgt cgccgacgtc aagcgcgccg
     2221 gcagcgacat taccctcgtc gcgttgagca gcatggtgca gaccgcgctc ggcgcggcga
     2281 agatgctcga ggaggtcggc atcaacgcgg aggtcgtcga tccccggacc acgtggccgc
     2341 tcgacgagaa gacgctcgtc gagtcggtca agaagacctc ccgctgcatc gtcgtcgacg
     2401 agggctactt ccggtacggc atcaccggcg agctcgcgtc ggtgatccag gagcaggcct
     2461 tctacgacct cgacggcccg gtcaggcggc tcggcgccat gcacgtgccg attccgttct
     2521 cgccggcgct ggaagacgtc acggtgccga gcgaacggca agtcttcgat ttggcgaggc
     2581 agatgtgtaa caggtaaagg gggtcgggag tcaattaacg aggacgtgcc ggaattcgtg
     2641 acgcgcctcg ttaaatgact cccgacccca tttgaaggag atccgacagt ggcaatcaag
     2701 acttacggca cgatggcggt ggactgggag cagcggatcg acttcgatcg gctgcggcgc
     2761 gagcggctcg cccggatcaa gggcctgctg gccaagtcgg agatgggctc ggtgctctgc
     2821 ttcgacatga
//
LOCUS       DBNGSH010000391         2014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_204894, whole genome shotgun sequence.
ACCESSION   DBNGSH010000391 DBNGSH010000000
VERSION     DBNGSH010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_204894"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="AC16CY_08965"
     CDS             <1..858
                     /locus_tag="AC16CY_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353958.1"
                     /translation="GYQGQGYLNHEQSPIPHLPRPQFTFNTTEGRASPSSAYAEPYPR
                     QPIPASEGKPGQPTLVLEPTQGPVGTHATLRGGGFPAGASLQLSWQTHVGSRVSGEGF
                     APDERVLGQVTVGRDGRVDAPVTIPEDLGGLHTLTLRNGDAALARASFAIETSIVGIS
                     PTSGPVGTPVTIHLKGVGWTEYDNISVATYDNAYMGYVCGFNSQGDVVINFTASGAPG
                     VHVIDLYPGIYQGPATEPQLLYRLPQLTYADDHPGNKIPALRFTFEVTGPGAGGWGQG
                     TAVRGSTSP"
     gene            938..1282
                     /locus_tag="AC16CY_08970"
     CDS             938..1282
                     /locus_tag="AC16CY_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3353959.1"
                     /translation="MTRKTQSRVDLLQGTLDLLLLRTLSAGPQHGHAIAKHIRQTSQD
                     LLQVETGSLYPALHRLEAGGWISAEWMLSDKGKRARFYRLTASGRRQLQNEQSKWQAF
                     ARAMGLILNPAE"
     gene            1289..>2014
                     /locus_tag="AC16CY_08975"
     CDS             1289..>2014
                     /locus_tag="AC16CY_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR3353960.1"
                     /translation="MKSLLRKLGWLLRRNQKEADLREELQFHLDEETEDRTADGLPIH
                     EARRAARLDLGNLAIVGEDTRAAWGWTLLEQLTQDVRYGVRMLAGNKVFSALAILSLA
                     LGIGANTAIFSFMDAMLMRSLPVSDPQSLVTLAWHTKKREMHGSNRHNVNYDDPRGGW
                     VGGIFSYPAFEFLQRETPVFSTVFGYQGAGDLHLAVNNQAAIVNAEYVSGNYFSGLGV
                     LPASGRLIAPDDDRAGAPAVAVIS"
BASE COUNT          366 a          670 c          649 g          329 t
ORIGIN      
        1 ggctatcagg gacagggcta tttgaaccac gagcaatcgc cgatcccgca ccttccgcgc
       61 ccgcagttca cgttcaacac gactgaaggc cgcgcgagcc cgtcctccgc gtacgcggag
      121 ccgtacccgc gccagccgat tccagcctcg gaagggaagc ccggacaacc gacgctggtg
      181 ctcgagccga cgcagggtcc ggtcggcacg cacgcgacgc tgcgcggcgg aggtttcccg
      241 gccggagcgt cgctgcagct ttcgtggcag acccacgtcg gcagccgggt gagcggcgaa
      301 gggttcgcac ctgacgaacg agtactcggc caggtcacgg tcggtcgcga cggacgcgtg
      361 gacgcgccag tgaccatccc cgaggacctc gggggattgc acacgctcac gcttcgcaac
      421 ggcgacgccg cgcttgcgcg cgcgtccttt gcgatcgaga cgagcatcgt cggcatctcg
      481 ccgacatccg gtcctgtcgg cactccggtc acgattcatc tgaagggcgt cggctggacc
      541 gaatacgaca acatctccgt ggccacctac gacaacgcct acatgggata cgtgtgcggc
      601 ttcaacagcc agggcgacgt cgtgatcaac ttcaccgcga gcggcgcacc cggggtccac
      661 gtcatcgatc tctatcccgg tatctaccag gggccggcca ccgagccgca gttgctctac
      721 cgccttccgc agctgaccta cgccgacgat caccccggca acaagattcc cgcgcttcgc
      781 ttcacgttcg aggtcacggg tccgggggca gggggctggg ggcaggggac agcggttagg
      841 ggaagtactt cgccgtaggc gctgaagagc cgcgttcgaa ggcggctcga acattctgta
      901 gacatcctac gcaagtattg tgtaggatgc ctacgctatg acccggaaga cccaatcccg
      961 cgtcgatctg ctgcaaggca ccctcgatct gctgttgctg cggacgctgt ccgccgggcc
     1021 gcaacacggc cacgccatcg cgaaacacat ccgccagacg tcgcaagacc tcctgcaggt
     1081 ggagaccgga tcgttgtatc ccgcgcttca ccggctcgaa gccgggggct ggatctcggc
     1141 ggagtggatg ctgtccgaca agggaaagcg cgcccgtttc taccgcctca ccgcgtcggg
     1201 gcgcaggcag ctccagaacg agcagtcgaa gtggcaggcg ttcgcgcgtg ccatgggctt
     1261 gattctcaac ccggcggagt aaagcgccat gaaatccctc ctgcgcaagt tgggctggct
     1321 gctgcgacgc aaccagaaag aggcggatct tcgcgaagag ctgcagttcc atctcgacga
     1381 ggaaacggaa gaccgcacgg ccgacggtct cccgatccac gaggcgcgtc gcgccgctcg
     1441 tctggacctg ggcaatctcg ccatcgtcgg cgaggacacg cgcgcggcat ggggctggac
     1501 actgctggaa cagcttacgc aggatgtgcg ctacggcgtg cgcatgctgg cgggaaacaa
     1561 ggtgttcagc gccctcgcga tcctgtcact cgcgctcggg atcggcgcga acacggcgat
     1621 cttcagcttc atggatgcca tgttgatgcg gtcgctgccg gtatccgacc cgcagtcgct
     1681 cgtcacgctg gcctggcaca cgaagaagcg ggagatgcac ggctcgaacc ggcacaacgt
     1741 caactacgac gatccacgcg gcgggtgggt cggcggaatc ttctcgtacc cggcattcga
     1801 gttcctccag cgcgagactc ccgtgttctc gacggtcttc ggataccagg gcgcgggcga
     1861 cctccacctt gccgtcaaca atcaggccgc gatcgtcaat gccgagtacg tctcggggaa
     1921 ttacttcagc ggtctcggcg tgctgcccgc atcgggacgg ctgatcgctc cagacgacga
     1981 tcgcgccgga gcaccggcgg tggcggtcat cagc
//
LOCUS       DBNGSH010000392         1691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_884331, whole genome shotgun sequence.
ACCESSION   DBNGSH010000392 DBNGSH010000000
VERSION     DBNGSH010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_884331"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="AC16CY_08980"
     CDS             <1..1350
                     /locus_tag="AC16CY_08980"
                     /inference="COORDINATES: protein motif:HMM:NF040438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HIR3353961.1"
                     /translation="TLCVSTLAGQGGQLPGGGRGRAGGPPPAPSNLPDTPTAVALPTV
                     SAEITGPGPIFDSVPSLPPGKGLAAYGYQAREYFVSGTANGEPYTTRIVVRKPADASK
                     FSGLVLAEAMHGSGAAHMFEFTSIYTMSSGHAAVEILTTPPAQFVQLNEARYKDLKLT
                     GAQTNEILAQIGSLVRSGSLLGGPVRKMVLGGTSMTAGVLINYLPAHMVYRTPQMQRI
                     YDGFLPMSNGALIREVDVPLIHVPTMHEVSGNITNRQDGDEPGKQYRLYEFSGMGHID
                     TRDSVRMQPNPCVDPLSTFPVQAYMSVALDHLLKWVDAGTVPPRADRIWLDRNQFNDG
                     SMMALDEHGNPRGGVRTTYVDVPAVKYVIRPAAITPVIPNAGAYIASRGQTAANQMCG
                     LSTAQIAIAPEKLKALYKNKQGYVKAVEARLTALEKAGWSLPLYRELILSDAAKVNF"
     gene            1366..>1691
                     /locus_tag="AC16CY_08985"
     CDS             1366..>1691
                     /locus_tag="AC16CY_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3353962.1"
                     /translation="MRRSLLWLAGVLMLIHACASAPPPPNEADIQAVVEGFYGAMRKG
                     DAAAVMSLIAPDAMFVESGRLETRAEYESNHLPSDIDFETQVAGKRGPLRVTFEGNTA
                     WVIATT"
BASE COUNT          292 a          565 c          570 g          264 t
ORIGIN      
        1 acgctgtgcg tgtccacgct cgccgggcag ggcgggcagc ttcctggcgg cggccgaggc
       61 agggcaggcg ggcctccgcc ggcgccgtcg aatcttcccg acacgcccac ggccgtcgcg
      121 ctgccgacgg tgtcggctga gatcaccggg ccaggtccga ttttcgattc ggtgccctcg
      181 ctcccgcccg gcaaggggct ggccgcgtac gggtaccagg cgcgcgagta tttcgtgtcg
      241 ggaaccgcca acggcgagcc ctacaccacg cgcatcgtcg tgcgaaagcc ggccgacgcg
      301 tcgaagttca gcggcctcgt gctcgccgag gcgatgcacg ggagcggcgc cgcccacatg
      361 ttcgagttca cgtcgatcta cacgatgtca tcgggccacg cggccgtgga aatcctgacg
      421 acgccgcccg cgcagttcgt gcagctcaac gaggcgcgct acaaggatct caagctcact
      481 ggcgcgcaga ccaacgagat cctggcccag atcgggtcgc tcgtccggag cgggagcctg
      541 ctcggcggac cggtgaggaa gatggtgctc gggggaacgt cgatgacggc cggcgtgctc
      601 atcaactacc tcccggcgca catggtctac cgcacgccgc agatgcagcg gatctacgac
      661 gggttccttc cgatgtcgaa cggcgcgctg atccgcgagg tggacgtccc gctgattcac
      721 gtgcccacga tgcacgaggt cagcgggaac atcaccaatc gccaggacgg cgacgagccc
      781 gggaagcagt accggctcta cgagttctcc ggcatggggc acatcgacac gcgcgacagc
      841 gtgaggatgc agccgaaccc ctgcgtcgat cctctcagca ccttcccggt gcaggcgtac
      901 atgtcggtcg ccctggatca cctgttgaag tgggttgatg cgggcacggt gccgccgcgc
      961 gcggatcgca tctggctgga tcgcaaccag ttcaacgacg gctcgatgat ggcgctcgac
     1021 gagcacggaa acccgagggg cggggtccgg acaacctacg tggacgtgcc ggcggtgaag
     1081 tacgtgattc ggcccgcggc catcacaccc gtgattccca acgccggcgc ctacatcgcg
     1141 tcgcgcggac aaaccgcggc gaatcagatg tgcggtctca gcaccgcgca gatcgccatc
     1201 gcgccggaga agctcaaggc gctctacaag aacaagcagg ggtacgtgaa ggccgtcgag
     1261 gcgcggctca cggcgctcga gaaggccggc tggtcgcttc cgctgtaccg cgagctgatt
     1321 ctgtcggacg ccgcgaaggt caacttctag cgtcgcggtg ctttgatgcg acgctcactt
     1381 ctctggcttg ccggcgtcct gatgctgatt cacgcgtgcg cgtctgcgcc gccgcctccg
     1441 aacgaggccg acatccaggc tgtcgtcgaa gggttctatg gagcgatgcg gaagggcgac
     1501 gccgccgcgg tgatgagcct gatcgcgccc gacgcgatgt tcgtcgagtc cggcaggctc
     1561 gagacccgcg ccgagtacga atcgaaccat cttccctcgg acatcgactt cgagacgcag
     1621 gtcgcgggca agcgcggccc cctgcgcgtg acgttcgaag gcaataccgc ctgggtgatc
     1681 gccaccacag a
//
LOCUS       DBNGSH010000393         6995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1902696, whole genome shotgun sequence.
ACCESSION   DBNGSH010000393 DBNGSH010000000
VERSION     DBNGSH010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1902696"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC16CY_08990"
     CDS             <1..333
                     /locus_tag="AC16CY_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3353963.1"
                     /translation="ALMTVCLLSLGGLPPTAGFIGKWYIFSAAVSAGYYGLAIVGVLT
                     SVISVFFYLRVVVMMYMAERGGAPAPAPVSGVGIAVLALSTAAIIFLGILPAPVLDLA
                     AASISTIF"
     gene            793..1380
                     /locus_tag="AC16CY_08995"
     CDS             793..1380
                     /locus_tag="AC16CY_08995"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HIR3353964.1"
                     /translation="MDEPSDIDQELTLLESEIKKLEAQYNMFFAGRLPRPPWESRKRV
                     EILVRRLDRLHIANYGQRFKFTTLQSRFSTSVDLWDRGLRAREEGRPGPFAQPMPVAE
                     KPKPRPADHVVHVTTLRDPSKEPDKLRELYDNLAEARREVGQDAISFQKFADLIKTQM
                     TTLKEKGNREVSFRVAVKDGKVAVTARGIKGKGEK"
     gene            complement(1437..1640)
                     /locus_tag="AC16CY_09000"
     CDS             complement(1437..1640)
                     /locus_tag="AC16CY_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR3353965.1"
                     /translation="MRITGKVKWFNNAKGYGFIEREGGSDVFVHYSAISGNGFRSLEE
                     GQAVEFEIVDGPKGPQAGNVTKP"
     gene            1889..3478
                     /gene="purH"
                     /locus_tag="AC16CY_09005"
     CDS             1889..3478
                     /gene="purH"
                     /locus_tag="AC16CY_09005"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR3353966.1"
                     /translation="MPRALLTVSDKTGLVELAGGLRGRGYELVSTGGTAEALSRAGLP
                     VKRVSEITGFPEIMDGRVKTLHPLIHGGILARRSDRAHLASAAEHGITLVDVVVVNLY
                     PFVAAASNPATPFDALVEEIDIGGPSLVRAAAKNFEGVIVVVSPADYGEVLRQLDRPG
                     GPSRAFRFELARKAFEHTASYDAAIASALGDVTVGETGFVRIAPGLPARMTIHLQKVR
                     DLRYGENPHQQGALYAGFGPHPGYVLLQGKELSYTNLLDLDAAIQIVIQFREPAAALV
                     KHTNPCGVATGSSAADAYVRAREADALSAFGAAVALNRPIDVETARAIVSTRIDAVIA
                     PSVDDEARPILAAKASMRVAVPERVPADSEAPAAQDFELRSIVGGVLVQSRDVVWEGL
                     EAWPSGDLTVVTKRRPTPDEWEALRFAWRVCAHVKSNTIVFTAKDRTLAIGAGQMSRV
                     DAANTARMKASAAGSNLLHGSVAASDAFIPFRDGLDAIAAAGATAVVQPGGSVKDDEV
                     IAAADEHGLAMVFTGRRHFRH"
     gene            3575..4162
                     /locus_tag="AC16CY_09010"
     CDS             3575..4162
                     /locus_tag="AC16CY_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353967.1"
                     /translation="MDATDARDHVRMLDGIVRATDRTVHVSPIVLIGIGAVCAAITGL
                     IQARQMGWNIPPDHYIQPPAFLVMLGIIAAASWRHRARGRDTLVDGYAGVAFLAAAAL
                     GLTLNVTAQDRVIPPAGIGLVWAGSFSMALLIIGAMGSRVLFVGAVAMLAAVGAAALV
                     PGWLPGILAIAWIVGFAVPGIVLALGAPHGRTAAL"
     gene            4140..4424
                     /locus_tag="AC16CY_09015"
     CDS             4140..4424
                     /locus_tag="AC16CY_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024988.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR3353968.1"
                     /translation="MDELLLSKIRLAVIGQLVTAQWVTFSELQGSIDVTNGNLGAHLA
                     KLVEAGYVKEEKRFEGRRPQSRYQLTKTGRSALLKHVAALRALIDSTTTH"
     gene            4569..4889
                     /gene="sugE"
                     /locus_tag="AC16CY_09020"
     CDS             4569..4889
                     /gene="sugE"
                     /locus_tag="AC16CY_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HIR3353969.1"
                     /translation="MAWVILVIAGLFEIGWAIGLKYTEGFARLWPTVLTAVSMVISLW
                     LLGVAMKSLPVGTAYAVWVGVGAVGTVVLGIVLLGESANAARLISVALIVAGIVGLKL
                     ATPV"
     gene            4915..5556
                     /locus_tag="AC16CY_09025"
     CDS             4915..5556
                     /locus_tag="AC16CY_09025"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR3353970.1"
                     /translation="MISATEALARLREGNLRFASNQSSVAAFDPARRAALVERQEPFA
                     IVLGCSDSRVPAELVFDQGLGDLFVIRVAGNIVAPSQVGSVEFAATRFGTRLVVVMGH
                     SQCGAITATLEEVLGQATTQSKNLRSIVDRVRPSIDAMIAARDEVRDLDALIHDAVRA
                     NVRASTAHLRHGSPLLERLIREEGLAVVGAEYSLESGIVAFFDGVPMSGREGK"
     gene            complement(5603..>6995)
                     /locus_tag="AC16CY_09030"
     CDS             complement(5603..>6995)
                     /locus_tag="AC16CY_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3353971.1"
                     /translation="SRHGIIVFGSTAGIMQVPASGGSPSAVTKAGEREAHTMPYFLPD
                     GRHFLYLRTGAPGSRAVQVGSLDAAPNEQNATALVKTDYGAVYAPGPRGSIGQLLFVR
                     GSTLLAQSFDADRLELEGEPVPVADPIATADFGIGIAYVSPSNTGTLLYFRPASPNVQ
                     LTWFDREGQAGRPAGEPGRWGVMKLSPDGTRAAIVRNDDGNGTDLWQIDLIKGTSTRF
                     TFDPGGDTQPVWSADGSKIAWISNRDGFIGIYSKPADGSGTEELLHKFKTRPNSLTDW
                     TRDGRFLVYTDGGDIWALPVSGVPPEKREPIPVIQGERGQLGAYVSPDQRWIAYISNE
                     SGQQEMYVQPFAPGTPGAKGQPVSGKWMVSSNGTLGMARWRADGRELIFLGTDGGVMS
                     LDIAEGPVFQASAPRLLFRLPLVLLTMTTQPGAIVDVTKDHKQVLVTMPIVDAFSGLN
                     IVMNWQSALAAQK"
BASE COUNT         1096 a         2335 c         2324 g         1240 t
ORIGIN      
        1 gcgctcatga ccgtgtgcct gctctcgctc ggcggcctgc cgccgacggc gggcttcatc
       61 ggcaagtggt acatcttcag cgccgccgtc agcgccggct actacgggct ggcgatcgtc
      121 ggcgtgctga ccagcgtgat ctcggtcttc ttctacctgc gcgtcgttgt gatgatgtac
      181 atggccgaac gcggcggcgc gccggccccg gcccccgtca gcggcgtcgg cattgcggtg
      241 cttgcccttt cgacggcggc catcatcttc ctgggaatcc tgcccgcgcc ggtcctcgat
      301 ctggcagccg cctcgatctc gacgatcttc tagagcggag ctcagttgta tgtagactgt
      361 cggcaagccc gcgagccgac gcggaaaaaa cgtgcttccg gcactcccgt cggcacacgt
      421 ttacctttct tgctagtggc cacgacctcg ccgcgcggcg agcctccagc acatttttat
      481 ccgatcgctc gtcgcgtcgg ctcgaagggc ttgccgccat tccacacaca tctgagctcc
      541 ggatactgct acgaccacga catcgtgctc cgtcacgtcc cgcgtgatct ggagccgtgg
      601 gggaccgttg gtccgacgca aaaccgtagt gggtgcattg gcctagccag ttggtcggcg
      661 gcgaacgctg agatgcgtgg atcaagtcgg ttttgcggag cgacccacgg tcccccccgg
      721 cgacgcgcat gaggatcact gcgcctctaa ggttcccgac ctgactctcg gctgtgccac
      781 aatgttcccc gcgtggacga gccatcggac atcgaccagg aactgacgct gctcgaatcg
      841 gagatcaaaa agctcgaggc gcagtacaac atgttcttcg cgggccggct gccgcgtcca
      901 ccgtgggagt cgcgcaagcg cgtcgagatc ctggtcagga ggctcgatcg acttcacatc
      961 gccaattacg gccagcggtt caagttcacg acgcttcagt cgaggttttc gacctcggtg
     1021 gacctctggg atcggggact gcgcgcgcgg gaagaagggc gccccggccc gttcgctcag
     1081 cccatgccgg ttgcggagaa gccaaagccg cggccggccg atcacgtcgt gcacgtgacc
     1141 acgcttcgcg atccgtccaa ggagccagac aagctgcgcg agctgtacga caacctggcg
     1201 gaggcgcgcc gcgaagtcgg ccaggacgcg atttcgtttc agaagttcgc cgacctgatc
     1261 aagacgcaga tgacgacgtt gaaggagaag gggaaccgcg aagtgtcgtt ccgcgtggcc
     1321 gtgaaggacg gaaaagtcgc cgtgaccgcc cgcggaatca aggggaaagg ggagaagtag
     1381 gagaggccga agagaacccg gcgcctgggg cggcgccggg caggtcgtgc gtggttctaa
     1441 ggcttggtga cgttccccgc ctgcggacct ttggggccgt cgacgatttc gaactcgact
     1501 gcttgcccct cttcgaggga tcggaacccg ttgccgctga tcgcggagta gtgcacgaac
     1561 acgtcgctgc caccctcacg ttcgatgaac ccatagccct tggcgttgtt gaaccacttg
     1621 accttacctg tgatacgcat cgttcttctt tcctacgtgc aaggcgggct cccttgcgga
     1681 caaaggtccc gctcgatcgc gtgccctgcg aagctgcaga tgagcttcgg agcacaaaaa
     1741 aaagaccgcg agtgcttgcg cggcctcgac tacaacatcc ggggaacacc ccgcgaacgc
     1801 aggcatatgc taagcgcctg ttacaggcct gtcaagtctt agtcggggct aagtgctgct
     1861 ttttcctgag gatgcacaca caacgtccat gccgagagcg ctgttgaccg tttcggacaa
     1921 aaccgggctc gtcgagctgg ccggcggctt gcgcgggcgc ggttacgaat tggtatcgac
     1981 gggggggacg gctgaggcgc tgtcgcgcgc cggccttccc gtgaagcggg tgtcggagat
     2041 cactggcttt ccggaaatca tggacggccg cgtcaagacg ctgcacccgc tgatccacgg
     2101 cggcatcctg gcgcgccgat ccgatcgcgc gcacctggcg tcggccgccg agcacggcat
     2161 cacgctcgtg gacgtggtcg tcgtgaatct ctacccgttc gtcgcggcgg ccagcaaccc
     2221 cgcgacgccg ttcgacgcgc tcgtcgaaga gatcgacatc ggcggaccca gccttgtgcg
     2281 cgcggccgcc aagaacttcg agggcgtgat cgtcgtggtg tcgccggccg actacggcga
     2341 ggtgctgcgg cagctcgatc gccctggcgg tccgtcgcgc gccttccggt tcgagctggc
     2401 gcgcaaggcg ttcgagcaca cggccagcta cgacgcggcg attgcgtccg ccctcggcga
     2461 cgtgaccgtt ggcgagacgg gcttcgtccg catcgcgccc ggactgcctg cgcgcatgac
     2521 gatccacctc cagaaggttc gcgacctgcg ctacggcgag aacccgcacc agcagggcgc
     2581 tctctacgcg gggttcgggc ctcatcccgg ctacgtcctc ctgcaaggca aggagctgtc
     2641 gtacacgaac ctgctcgacc tggacgccgc catccagatc gtcatccagt tcagggaacc
     2701 tgcggcggcg ctcgtcaagc acacgaaccc gtgcggcgtc gccacgggat cgagcgcggc
     2761 ggatgcctac gtccgcgcgc gcgaggccga cgcgctctcc gcattcggcg cggcggtcgc
     2821 gctcaaccgt ccgatcgacg tcgagacggc tcgcgcgatc gtgtcgacgc gaatcgacgc
     2881 cgtgatcgcg ccgtcagtcg acgacgaggc gcgcccgatc ctcgctgcca aggccagcat
     2941 gcgcgtggcc gtgcccgagc gcgtgcccgc cgactcggag gccccggcgg cccaggactt
     3001 cgagctgcgc tcgatcgtcg gcggcgtgct ggtgcagagc cgggacgtcg tgtgggaagg
     3061 gctcgaggcc tggccgtccg gcgatctcac ggtggtgacg aagcgtcggc caacgcccga
     3121 tgaatgggag gcgctgcgct tcgcctggcg cgtgtgcgcg cacgtcaagt cgaacacgat
     3181 cgtcttcaca gcgaaggatc gcacgctggc catcggcgcg gggcagatga gccgcgtcga
     3241 tgccgccaac accgccagga tgaaagcgtc ggccgccggc tcgaatctgc tccatggttc
     3301 ggttgcggcc tccgatgcct tcatcccgtt tcgcgacggg ctcgacgcca tcgccgccgc
     3361 cggggcgacc gccgtcgtgc agccgggcgg ctcggtcaaa gacgacgagg tgattgcagc
     3421 cgccgacgag cacggcctcg cgatggtgtt caccggtcgg cggcacttca ggcactgacc
     3481 tcggaggcgc atcacgtggg gcttcaggcc cttgacgcgc caccatggtc tagattagtc
     3541 tgtatgacag attagtcttc acataggagc ccgtatggac gccactgacg cgcgcgacca
     3601 cgtgcgcatg ctcgacggca tcgttcgcgc gaccgaccgg accgtacacg tctcgcccat
     3661 cgtgctgatc ggcatcggcg ccgtctgcgc ggcgatcacc ggcctgatcc aggcgcggca
     3721 gatgggatgg aacatcccgc ccgaccacta catccagccc ccggcgttcc tggtcatgct
     3781 gggaatcatc gccgccgcct cctggcgcca tcgcgcccgc gggcgcgaca cgctggtcga
     3841 cggctacgcc ggggtcgcgt tcctcgccgc cgccgcgctc gggctcacgc tgaacgtgac
     3901 ggcgcaggac cgcgtcattc cgcctgcggg catcggcctc gtctgggcgg gcagcttcag
     3961 catggcgctg ctcatcatcg gcgcgatggg cagccgcgtc ctgttcgtcg gcgccgtcgc
     4021 gatgctcgcc gccgtcggcg cggccgccct cgtgcccggc tggctcccgg gaatactggc
     4081 gatcgcgtgg atcgtcgggt tcgccgttcc cggcatcgtc ctcgcgctcg gggcgccgca
     4141 tggacgaact gctgctctct aagatccggc tcgccgtgat cggccagctg gtcaccgcgc
     4201 agtgggtgac cttcagcgag ctgcaggggt cgattgacgt gaccaacggc aacctcggcg
     4261 cgcacctcgc aaagctcgtc gaggccggct acgtcaagga agagaaacgc ttcgaaggcc
     4321 gccgcccgca gagccgctat cagctgacga agacggggcg ctccgcgctc ttgaagcacg
     4381 tggcggcgtt gcgggcgctc atcgattcaa ccacgaccca ttgacgcgac tggcacgtcc
     4441 gtacgctggt cgctgtcccg ggagcgattg gtcgtcgact acgggcgcta tcgacgtttt
     4501 tcggtcgaca cgaagtggtt ggtccagtga tcacggcgcg gcgcttcagg cactaagatt
     4561 gaaaggtcat ggcttgggtc attctcgtca tcgcggggct gttcgagatc ggctgggcga
     4621 tcgggttgaa gtacaccgag ggcttcgccc ggctgtggcc gacggtcctg actgccgtgt
     4681 cgatggttat cagtctctgg ctgctgggag tcgccatgaa gtcacttcca gtgggcacgg
     4741 cgtacgccgt gtgggtcggc gttggagcgg tcggcaccgt tgtgctggga atcgtactgc
     4801 tcggagagtc tgcaaacgcc gcccggctca tcagcgttgc gctcatcgtc gccggcatcg
     4861 tcgggttgaa gctcgcgaca ccggtctgac cacgcgcaga cgcccgtccc tgtcatgatt
     4921 tccgcaacag aggctctcgc gcgtttgcgc gaggggaacc tccgcttcgc cagcaaccag
     4981 tcgtccgtcg cggcgttcga tcccgccagg cgtgccgcgc tggtcgagag gcaggagccg
     5041 tttgccatcg tgctgggatg ttcggactcc cgcgtgcccg cggagctggt tttcgaccag
     5101 ggactcggtg acctctttgt gatccgggtc gccggcaaca tcgtggcgcc ctcgcaggtc
     5161 ggcagcgtgg agttcgcggc cacgcgattc ggcacgcgac tggtcgtcgt gatgggtcac
     5221 tcgcagtgcg gcgccatcac ggcaacgctc gaggaggtcc tcgggcaggc gacgacccag
     5281 tcgaagaacc tgcgttccat cgtggaccgc gttcggccgt cgatcgatgc gatgattgcc
     5341 gctcgagacg aggtgcgtga cctcgacgcc ctgatacacg acgctgttcg cgccaacgtc
     5401 cgcgcgtcaa ccgcgcacct gcgacacggc tcgccgctcc tggagcggtt gattcgcgag
     5461 gagggcctgg ccgttgtcgg cgcggaatat tcgctggagt cgggcatcgt ggcgttcttc
     5521 gacggcgtac cgatgtccgg ccgcgaaggg aagtagcctt cgaccgcccc ttggcgttgc
     5581 gctggcgcgc gaccgtcggc gtctacttct gagccgccaa cgccgactgc cagttcatca
     5641 cgatgttcaa ccccgaaaag gcgtccacga tcggcatggt caccagcacc tgtttatggt
     5701 ccttggtgac gtccacgatg gcgcccggtt gggtggtcat cgtgagcagg accagcggca
     5761 gccggaacaa gagccgcggg gcgctcgcct ggaagacggg cccctcggcg atgtccagcg
     5821 acatcacacc accgtccgtg ccgaggaaga tcagctccct gccgtcggcg cgccatcgcg
     5881 ccatgccgag cgtgccgttg gatgacacca tccacttgcc cgacaccggc tgtcccttcg
     5941 cccccggcgt tcccggggca aacggctgca cgtacatttc ctgctggccc gactcgttcg
     6001 agatgtaggc gatccaccgc tggtcggggg agacgtacgc gccgagttgt ccccgctcgc
     6061 cctggatcac tggtatcggc tcgcgcttct caggcggaac gccggacacc gggagcgccc
     6121 agatgtcgcc cccgtcggtg tacacgagga agcgcccatc gcgcgtccag tccgtcagcg
     6181 aattcggcct cgtcttgaac ttgtggagca gttcttcggt gcccgatccg tcggcaggct
     6241 tcgagtagat gccaatgaag ccgtcgcggt tcgagatcca cgcgatcttg cttccgtcgg
     6301 ccgaccagac cggctgcgtg tcccccccgg gatcgaacgt gaaccgcgtg ctcgtcccct
     6361 tgatcaagtc gatctgccag aggtccgtgc cgttcccgtc gtcgttgcgg acgatcgcgg
     6421 ccctcgttcc atcgggtgag agcttcatca cgccccagcg cccgggctcg cctgccgggc
     6481 ggcccgcctg cccttcgcgg tcgaaccacg tgagctgcac gttcggagag gcaggcctga
     6541 agtacaggag cgttcccgtg ttcgacggcg agacgtaggc gattccgatt ccgaagtccg
     6601 ctgtggcgat cgggtctgca actggaactg gctcaccctc gagctcgagc cggtccgcgt
     6661 cgaaggactg cgccagcagc gtgctgccgc ggacgaagag gagctgaccg atgctcccgc
     6721 gcggtcccgg cgcgtagacc gcgccgtagt cggtcttaac gagtgccgtc gcgttctgct
     6781 cgttcggtgc cgcgtcaagc gacccgacct gaacggcgcg gctgcctgga gcgccggtcc
     6841 gaaggtagag aaagtgccgg ccgtcaggga ggaagtacgg catcgtgtgc gcctcgcgct
     6901 cgccggcctt ggtgactgcg gacggtgacc cgccagaggc gggcacctgc atgatcccgg
     6961 cggtgctccc gaagacgatg atgccgtggc gactc
//
LOCUS       DBNGSH010000394         7953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2242960, whole genome shotgun sequence.
ACCESSION   DBNGSH010000394 DBNGSH010000000
VERSION     DBNGSH010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2242960"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="AC16CY_09035"
     CDS             <1..1287
                     /locus_tag="AC16CY_09035"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF037084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR3353972.1"
                     /translation="EIAPVFENANLAVDHQMMTMEMLKKIAPKYGLSALLHEKPFAGV
                     NGSGKHNNWSLSDEFGNNLLGPGDTPHDNMQFLVFCAAVLRAVDRWQGLLRASIASAG
                     NDHRLGANEAPPAILSVFLGEMLTDIFQQIEKNGGATSTKQGGIQDLGVSVLPKLPRD
                     AGDRNRTSPFAFTGNKFEFRAVSSGQSIAFPNMVLNAAMADSLDSIANELEAAKAKGE
                     ELNKTVGKLLTKIIKEHKRIIFNGNNYAPEWEKEAGRRKLLNLKNTVDALPELVTKDA
                     TRLLDRFKILNERELHARYEIFLENYNKTINIEGQLMVLMANRYILPAALEYQKQIGQ
                     SVAAVKAGGGRSAQAKKLLGTYTKLVDRLGLETQALAGLLDHSSNGSSEKHAKFMRDK
                     IVPAMAKLRTTGDEIEVLTPHAIWPLPTYREMLFVK"
     gene            1595..4447
                     /locus_tag="AC16CY_09040"
     CDS             1595..4447
                     /locus_tag="AC16CY_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HIR3353973.1"
                     /translation="MGILEKLRPQPRWKHADPGVRAAAVYEMGPDEVDLLLGLVREDA
                     DSRVRRAAVIRLSDVEVLAEVARTDPDEDVRAEAIRGLTGLAAETDDPAPASDVVRQL
                     QALGRSKEVVVVVRENTSAEVRAAVVGLIEDAVTLGSVSRHAQDGQTRLRALGRLQDA
                     DEILNVALKSEHTDVAVSALERIDDPAALSSIAQRARNKVAARRARTKLRRLEEDSQP
                     APSASMSADDRARAVDLLHQAEALVATPDPEEASAALGRVRLAWAELQADVEVDAALV
                     QQFESASDAVREGIEARRREAVAERERERALAREQADRLAIVEEIERISGPGALDRIA
                     ELRVKWDELPPMPPEFAASLTLRFQDASRAFEDRERRRLLSEAAAVRLDTLATELEQL
                     ASSEQAVDEVVARWRGLRRDADVLREHADANVQAAERLERVVAVLEQKEVEHQQLRAK
                     REQDNLRRLQQCCRQADALAASDQLTLKAGDRALREIRAALDERAPLPSKRDRQEIQA
                     RLEDVRTRLAPRVQELREADEWQRWANLQVQESICRDMEALKAEDDVEVAARRMRELQ
                     SRWKQVALAPRAQGEVMWRRFKTAQDEVFGRTAAFFAAQQAERDANLVKKQALCERVE
                     ALAGSTDWVKTAAAIQSVQGEWKTIGPVARGHEKAVWERFRKACDEFFTRRQQDLKQR
                     KEEWAANLARKEALCASAEELADSTDWESTAARIKRLQAEWKRVGPVRKSKSDALWQR
                     FRTACDRFFERYKHRDQVELQEKAVVRETLVRELEALTTPESTNGGPDNLYQTLLQAR
                     ARWQQAPELPRAIQQDLAARYHQAVARIVSAWPAAFAGTDLDPEATRKRMERLLAKVE
                     ELASAHAGRPANLSPAELLAQQWRDRLAANTIAGGGARVAETEESRWRAIEQEVRAVQ
                     AQWMRLGPVPPHVAGPLNERFQRACRRFFDERRRAS"
     gene            complement(4462..6378)
                     /gene="speA"
                     /locus_tag="AC16CY_09045"
     CDS             complement(4462..6378)
                     /gene="speA"
                     /locus_tag="AC16CY_09045"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HIR3353974.1"
                     /translation="MPSVVTSTTRAWSVHDASELYEVGRWGKGYFSVNDLGHLQAHPT
                     KEPSRAIDLKELVDRLQLRGIGLPVLLRFTDILKHRLADIHAAFQGAIAQHQYQGGYS
                     CVYPIKVNQQRQVVEEVLNFGRPFNFGLEAGSKPELLAVVALADNATPIICNGFKDAE
                     FIETAMFGHQIGRNIIPVVEKYTELGLILDAADKLGVRPQIGMRVKLASRGSGRWQSS
                     AGFRSKFGLTVTEIMRGLEELKLRGMQDCLRLLHFHLGSQITNIRIVKGALNEAARVY
                     TELAKLGAGLRYLDVGGGLGVDYDGSQTNFESSMNYTLEEYANDVVYHIQSVCDEAGV
                     AHPTIISESGRAVVAYHSMLVFNVLGVSAFGDEQIPTTPNLEWEQPVADLIETYHNVT
                     ERNALEAFHDAQQALDMALSLFNTGYMPLVQRSIAENLFWAICTKLQRITQTMAEVPE
                     DLQNLDEQMSDTYFCNFSLFQSIPDSWAIKQIFPVMPIHRLNQKPTKYAVLGDITCDS
                     DGKLDRFVDRRDVKKTLPLHTVNGDPYYLGVFLVGAYQEILGDLHNLFGDTHAVHVSF
                     DERGAAKLDALVKGDTVREVLDYVEFDSELLLGKMRTDVETAVREGRIDNEAAGRLLR
                     FYEDGLHGYTYLEG"
     gene            6518..6925
                     /locus_tag="AC16CY_09050"
     CDS             6518..6925
                     /locus_tag="AC16CY_09050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3353975.1"
                     /translation="MTQRVPAIRVLLLPKDTNAHGTIFGGVILSNIDLASAIEARKTA
                     AHRFVTRAMREVEFHQPVYVGDIVDFFTETVRIGRTSVTVKVWVEAERWGNPPAAAGA
                     TGHGERVKVTEAEVVLVAVDGEGRPVPVRPDGG"
     gene            6954..>7953
                     /gene="thrC"
                     /locus_tag="AC16CY_09055"
     CDS             6954..>7953
                     /gene="thrC"
                     /locus_tag="AC16CY_09055"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3353976.1"
                     /translation="MKWISSRGGSPPISFIDALFAGTAPDGGLYMPERLDPLPRGTVE
                     RLRGAGIVETGTTIGAHLLADEITPEALAPLVRDALNFPIPLVQVAERTWVLELFHGP
                     TMAFKDVGARVQARLLHHFTDGTPLTILVATSGDTGSAVAQAFYRVPDARVVVLYPEG
                     KVSDVQEAQMASLGENVSALAVHGTFDDCQRLVKQAFADDRLRQHVWLTPANSINLGR
                     LLPQVFYYFVLSSLAGPDGSPAPGPIVSVPSGNFGNLTAGLIAKRIGLPIARFVAATN
                     VNDAVPAYLASGLYEPRPSIRTVANAMDVGAPSNFERVQALYGGDLAALRRDVAGFA"
BASE COUNT         1364 a         2575 c         2725 g         1289 t
ORIGIN      
        1 gaaatcgcgc cggtcttcga gaacgccaac ctcgccgtcg atcatcagat gatgacgatg
       61 gagatgctga agaagatcgc gccgaaatac gggctgtcgg cgctcttgca cgagaagccg
      121 tttgccggcg tgaacggatc gggcaagcac aacaactggt cgctctccga cgagttcggc
      181 aacaacctgc tcggccccgg cgacacgccg cacgacaaca tgcagtttct cgtcttctgc
      241 gcggcggtcc tgcgcgccgt cgatcgctgg cagggacttc tgcgcgccag catcgccagc
      301 gccggcaacg atcatcgcct tggcgcgaac gaagcgccgc cggcgatcct ctcggtcttc
      361 cttggcgaga tgctgaccga catcttccag cagatcgaaa agaacggcgg ggccacgtcc
      421 accaagcagg gtgggattca ggatctcggc gtcagcgtgc tccccaagct gccgcgggac
      481 gcgggcgacc gcaaccgcac cagcccattc gccttcaccg gcaacaagtt cgagttccgc
      541 gccgtgtcgt cagggcagag catcgccttc cccaacatgg tcctgaacgc ggcgatggcc
      601 gactcgctcg actcgatcgc gaacgaactg gaagccgcga aggcgaaggg cgaggagctg
      661 aacaagacgg tcggcaagct gctgacaaag atcatcaagg agcacaagcg catcatcttc
      721 aacggcaaca actacgcgcc ggagtgggag aaggaggctg gcaggcgcaa gctcctgaac
      781 ctcaagaaca ccgttgacgc cctcccggag ctcgtcacca aggacgcgac caggctgctg
      841 gatcggttca agatcctgaa cgagcgcgag ctgcacgccc gctacgagat cttcctcgag
      901 aactacaaca agacgatcaa catcgagggg cagctcatgg tgctgatggc gaaccggtac
      961 atccttccgg ccgcgctcga gtaccagaag cagatcggcc agagcgtggc cgcggtcaag
     1021 gccggcggcg gccgcagcgc gcaggccaag aagctgctgg gcacttacac caagctggtg
     1081 gaccgtctcg ggctggagac ccaggcactc gcggggctgc tcgatcactc gagcaacggg
     1141 tcgagcgaga agcacgcgaa gttcatgcgc gacaaaatcg ttccggcgat ggcgaagctg
     1201 cggaccacag gcgacgagat tgaggtcctg acgcctcacg caatctggcc gttgccgacc
     1261 tacagggaga tgctatttgt aaaatagaag gggtcagggg ggaagggggc agggggcaga
     1321 aggctccttt ctgagttccc ctttcccttc cccctgcccc ctatcccctg cccccttcag
     1381 agttcccttc aagttctcag attcccggat ccaaaaagtc aaatcagtct gcaacctcgt
     1441 gccttcggcc gtgcttcgcg ggcacacggc ctacgtgccg caatcatgca atttccagtc
     1501 attcctgacg aacgcgaggg cggcgatcgg tggttcttcg ccggccatgc cggctattga
     1561 aactcgccta ggtcttgcaa gggacgacag agacgtgggg atcctcgaga agctccgtcc
     1621 tcagcctcgc tggaaacacg ccgaccccgg ggtgcgtgcg gccgctgtgt acgaaatggg
     1681 tcctgacgag gtcgatctgc tgctcggcct cgtccgcgag gacgccgatt cccgagtcag
     1741 gcgcgcagcc gtaatccggc tgtcggatgt cgaggtcctc gcggaggtcg cacgaacgga
     1801 ccctgacgaa gacgtgcgcg ccgaggccat ccgggggctg accggtctgg ccgccgagac
     1861 ggacgatccc gcgccggcca gcgacgtcgt ccggcagctt caggcccttg ggcggtcgaa
     1921 ggaagtcgtg gtcgtggtgc gcgagaacac cagcgccgag gtgcgcgcgg cggtcgtcgg
     1981 cctgatcgag gatgccgtga cgcttggatc cgtgagccgc cacgctcagg acggccagac
     2041 gcggctgcgc gccctgggac gcctgcagga tgcggacgag atcctcaacg tcgcgctgaa
     2101 gtctgaacat actgacgtgg ctgtgtcggc gctcgagcgg atcgacgatc cggcggcgct
     2161 gtcgtctatt gcccagcgcg cccggaacaa agtggccgcg cgccgcgccc gtacgaagct
     2221 gcggcggctg gaggaagaca gtcagccggc gccttcggcg tcgatgagcg ccgacgatcg
     2281 cgcgcgggcc gtggacctgt tgcatcaggc cgaagccctc gtggcgacgc ccgatccgga
     2341 ggaggcctcg gccgcgctcg gccgcgtccg cctggcatgg gcggagctgc aagccgacgt
     2401 cgaggtggac gccgcgctcg ttcagcagtt cgagtcggcc agcgacgccg tgcgtgaagg
     2461 gatcgaggcg cgtcgccgcg aagcggtcgc ggagcgcgaa cgagagcgcg cgctcgcgcg
     2521 cgagcaggcc gaccgcctgg ccatcgtcga agaaatcgaa cgcatcagcg gacccggcgc
     2581 gctcgaccgc atcgccgagc tccgcgtgaa gtgggacgag ttgccgccga tgccgcccga
     2641 gttcgcggcc tcgctgaccc tgaggtttca ggacgcgtcg cgggccttcg aggaccgcga
     2701 gcgtcggcgc ctgctgtcgg aagccgctgc cgtgaggctg gacacgctcg ccacggagct
     2761 ggagcagctc gcgtcgtccg agcaggcggt ggacgaggtc gtggcgagat ggcgtggcct
     2821 gcgtcgcgac gcggacgtgc tgcgcgagca cgccgacgcc aacgtgcagg ccgccgagcg
     2881 gctcgagcgt gtcgtggcgg tgctcgagca gaaagaggtc gagcatcagc agctgcgcgc
     2941 gaaacgcgag caggacaacc tgcggcgtct gcagcagtgc tgccggcagg ctgacgcgct
     3001 cgccgcaagc gaccagctca cgttgaaggc gggggatcgc gcccttcgag agatccgcgc
     3061 ggcgctcgac gagcgcgcgc cgctgccctc caagcgcgat cgccaggaga tccaggctcg
     3121 cctcgaggac gtccgcaccc ggctggcgcc gcgcgtccag gagctccggg aggccgacga
     3181 atggcagcgg tgggcgaacc tgcaggtgca ggagtcgatc tgccgcgaca tggaggcgct
     3241 gaaggccgaa gacgacgtgg aggtcgccgc acggcggatg cgcgagctgc agtcgcgatg
     3301 gaagcaggtg gcgctggcgc cgcgtgcgca gggcgaggtg atgtggcgcc gcttcaagac
     3361 ggcgcaggac gaggtgtttg gccgcacggc ggcgttcttc gccgcccagc aggcggagcg
     3421 cgacgcgaac ctcgtgaaga agcaggcgct ctgcgagcgc gtggaggccc tggccggatc
     3481 gaccgactgg gtgaagaccg ccgccgcgat ccagagcgtg cagggcgagt ggaagacgat
     3541 cggtcccgtg gcgcgcgggc acgagaaggc cgtctgggag cgcttccgga aggcgtgcga
     3601 cgagttcttc acgcggcggc agcaggacct gaagcagcgc aaggaggagt gggccgccaa
     3661 tctcgctcgc aaggaggcgt tgtgcgccag cgccgaggag ctggccgact cgaccgactg
     3721 ggagagcacg gccgcgcgga tcaagcggct ccaggcggag tggaagagag tcggtcctgt
     3781 ccgcaagtcg aagtccgacg ccctctggca gcgcttccgg acggcgtgcg accgtttctt
     3841 cgagcgctac aagcaccgcg accaggtcga gttgcaggag aaggcggtcg tgcgggagac
     3901 gttggtccgg gagctcgagg cgctgacaac gccggagtcg accaatggcg gccctgacaa
     3961 cctgtaccag accctgttac aggcacgggc gcgatggcag caggctcctg agctgccgcg
     4021 cgcgatccag caggatctgg cggccagata ccaccaggcg gttgcgcgga tcgtctccgc
     4081 gtggcctgcg gccttcgccg gcaccgacct cgatcccgaa gccacgcgca agcgaatgga
     4141 gcggctgctc gcgaaagtgg aggaattggc ctccgcacat gcgggtcgtc ccgccaatct
     4201 ttccccggcg gagctcctgg cgcagcagtg gcgtgatcgc ctcgcggcca acaccatcgc
     4261 cggaggcggc gcccgcgtcg ccgagaccga ggagtcgcgc tggcgcgcga tcgagcagga
     4321 agttcgcgcc gtccaggcgc agtggatgcg gctcgggccc gtcccgccgc acgtcgccgg
     4381 tccgctcaac gagcggtttc aacgggcctg ccggaggttc ttcgacgaac gccggcgcgc
     4441 gtcgtagcgc cttttgaacg ttcagccttc gaggtaggtg tacccgtgga ggccgtcctc
     4501 gtagaagcgg aggagccggc cggcggcctc gttgtcgatg cgcccctctc tgacggctgt
     4561 ctcgacgtcg gtgcgcatct ttcctagcag cagctctgaa tcgaactcga cgtagtcgag
     4621 cacctcgcgg accgtgtcgc ccttgacgag cgcgtcgagc ttggccgcgc cccgctcgtc
     4681 gaagctgacg tgcacggcgt gcgtgtcgcc gaagaggttg tgcaggtcgc cgaggatttc
     4741 ctggtaggcc ccgacgagga acacgccgag gtagtacggg tccccgttca cggtgtggag
     4801 cggcagcgtc ttcttgacgt cccggcgatc gacgaaccga tcgagcttgc cgtctgaatc
     4861 gcacgtgatg tcgccaagaa cggcatactt cgtcggcttc tggttcagcc gatggatcgg
     4921 catcaccggg aaaatctgct tgatggccca gctgtcggga atcgactgga agagcgagaa
     4981 gttgcagaag taggtgtccg acatctgctc gtcgaggttc tgcagatcct ccggcacctc
     5041 ggccatcgtc tgcgtgatgc gctgcagctt ggtgcagatc gcccagaaca ggttttccgc
     5101 gatgctgcgc tgaacgagcg gcatgtaacc cgtattgaag aggctcagcg ccatgtcgag
     5161 cgcctgctgg gcgtcgtgga acgcttcgag ggcgttgcgc tccgtcacgt tgtggtaggt
     5221 ctcgatcagg tccgcgaccg gctgctccca ctccagattc ggcgttgtcg ggatctgctc
     5281 gtcgccgaac gccgatacgc caaggacgtt gaagaccagc atgctgtgat aggcgacgac
     5341 cgcgcgtcca ctctcggaga tgatggtcgg gtgcgccacg cccgcctcgt cgcacacgct
     5401 ctggatgtgg tagacgacgt cgttggcgta ctcctcgagc gtgtagttca tgctcgactc
     5461 gaagttcgtc tgcgagccgt cgtagtccac gccgagtccg ccgccgacgt cgaggtacct
     5521 caggcccgcc ccgagcttgg ccagctccgt gtagacgcgg gcagcctcgt tgagcgcgcc
     5581 cttgacgatc cggatgttgg tgatctggct accgaggtgg aagtggagga gcctgaggca
     5641 atcctgcatg ccccgcagct tcagctcctc gagcccccgc atgatttcgg tgaccgtcag
     5701 gccgaacttg gaacggaagc cggcagacga ctgccagcgg ccgctgccac gcgaggcgag
     5761 cttgacgcgc atgccgatct gcggcctcac gccgagcttg tcggccgcgt cgaggatcag
     5821 gccgagctcc gtgtacttct cgacgaccgg aatgatgttg cgcccgatct ggtgcccgaa
     5881 catcgccgtc tcgatgaact ccgcgtcctt gaacccgttg caaatgatcg gtgtcgcgtt
     5941 gtcggccagc gccacaacgg cgagcagttc gggcttcgag ccagcctcga ggccgaagtt
     6001 gaacggcctg ccaaagttca ggacctcctc gaccacctga cgctgctggt tgaccttgat
     6061 cggataaacg cagctgtacc caccctggta ctggtgctgg gcaatggcgc cctgaaaggc
     6121 tgcgtgaatg tcagccaggc ggtgcttcag gatgtcggtg aagcggagga ggacagggag
     6181 gccgatcccc cggagctgca gacgatcgac cagctccttg aggtcgatcg ctcgcgaggg
     6241 ctcttttgtc gggtgggcct gcaagtggcc gaggtcgttg acggagaagt agcccttgcc
     6301 ccatcggccg acttcgtaga gctcgctggc gtcgtggacg ctccacgctc gcgtcgtcga
     6361 cgtcacgacg ctcggcagtg gacgtccatt cgtcagagtg ctcatgcctc cagccatgat
     6421 aaacgttatc ggccgtctgt ctcagaactt tgttgcggca gacacggctc ccgcactacc
     6481 aacctgaccg cgggacgcgt cacaatgtcc tcgtcccatg actcagcgcg tgccagccat
     6541 ccgggttctc ctgcttccga aggacaccaa cgcgcacggc actatcttcg gcggcgtgat
     6601 cctgtcgaac atcgacctcg cgtcggcgat cgaagcgcgg aagaccgctg cgcaccggtt
     6661 cgtgacccgg gccatgcgcg aggtggagtt ccaccagccc gtgtacgtgg gcgatatcgt
     6721 ggattttttc accgaaaccg tgcggatcgg ccggacgtcg gtgaccgtca aggtgtgggt
     6781 cgaggccgag cggtggggca acccgccggc cgcggcgggc gccacggggc acggcgaacg
     6841 ggtcaaggtg accgaggcgg aagtcgtcct cgtggccgtg gacggcgagg gccggcccgt
     6901 gcccgtcagg ccggatggtg gatagtggat agtggaccaa ccaccgaccg accatgaagt
     6961 ggatcagcag ccggggcgga tcgccgccaa tttccttcat cgacgccctc ttcgctggga
     7021 cggctcccga cggcggcctg tacatgccgg agcggctcga cccgctgccg cgcgggacgg
     7081 tcgagagact tcgaggcgcc gggatcgtcg agacagggac caccatcggt gcccacctcc
     7141 tggccgacga gatcacgccc gaggcgctcg caccgctcgt cagggacgct ctgaacttcc
     7201 cgattcccct ggtgcaggtg gccgagcgga cgtgggtgct cgagctcttc cacggaccca
     7261 ccatggcgtt caaggacgtg ggcgcgcgag tgcaggcgcg gctccttcac catttcaccg
     7321 acgggacccc cctcacgatt ctcgtcgcca cttccggcga cactgggagc gccgtggcgc
     7381 aggcgtttta ccgggtgccg gatgcccggg tggtggtgct ctaccccgag gggaaggtca
     7441 gcgacgtcca ggaggcccag atggcgtctc tgggcgagaa cgtgtcggcg ctggccgtgc
     7501 acgggacctt cgacgactgt cagcggctcg tcaagcaggc gtttgccgac gatcgcctgc
     7561 gccaacatgt ctggctgacc cccgccaatt ccatcaacct gggccggctc ctgccgcagg
     7621 tgttctacta ctttgtcctg tcgtctctgg ccgggccgga cggctcgccg gctcccggtc
     7681 ctatcgtctc ggtgcccagc ggcaacttcg gaaacctgac ggccggcctg atcgcgaagc
     7741 ggatcggcct gccgattgcc cggttcgtcg cggccactaa cgtcaacgac gctgtccctg
     7801 catatctggc gtccggcctc tacgagcctc gtccctccat tcggaccgtg gccaacgcga
     7861 tggacgtcgg ggccccgagc aacttcgagc gcgttcaggc gctctacggc ggcgacctgg
     7921 cggcgctgcg ccgtgacgtc gccggttttg cgg
//
LOCUS       DBNGSH010000395         1655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1903118, whole genome shotgun sequence.
ACCESSION   DBNGSH010000395 DBNGSH010000000
VERSION     DBNGSH010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1903118"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="AC16CY_09060"
     CDS             complement(<1..1359)
                     /locus_tag="AC16CY_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3353977.1"
                     /translation="MSKHLVRGHLRRVLAVAGMLIAYAIAGAAEQAPPQDQPRPTFRT
                     EANYIRVDAYATTRDGAAIGDLRQEEFRLLEDGVPQKIDSFSPVVIQAGGAPTTRTDP
                     RSPEESAQAAAEPGARVFVIFLDVMHVDYAASQTIAKPLIDALRRLIGPDDLVAIVNP
                     LTPVRAITFTRRVAAIEGTLSRPWGARDRLLSDPVEQRFAACYPGLPRIPGETVAPDL
                     GIAQEMILRRREAQTFDALDALVTELREIREERRAVITITHGWRVYRPNQALVRPIGP
                     AASPIPRPGFDPRTGQLSMPAAAGGVACEGDRMRLAQLDHTMRFRQLLDRANRANVSF
                     YPVDPRGVVPFDDDIVPVAGVGQNPMIPVVEDNNRLGERNTSIRTMAEYTDGVAVVST
                     NNFAPALQRITNDLSAYYLLGYYSTGKFDGKFHSITVSVTRPGVQIRARRGYLASVES
                     AVP"
     gene            complement(1417..>1655)
                     /locus_tag="AC16CY_09065"
     CDS             complement(1417..>1655)
                     /locus_tag="AC16CY_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353978.1"
                     /translation="VLDGARRLVLAEGGIFLEPTVVHLSGATFGGSTLKLGWILVGLR
                     VEFGVGGRQITTSPVQSVTIESPSPMEVPDQRAA"
BASE COUNT          298 a          537 c          545 g          275 t
ORIGIN      
        1 cgggaccgcc gattcaacac tcgcaaggta tcctcgcctc gccctgatct ggacgccggg
       61 ccgggtcaca ctcaccgtga tggagtgaaa cttgccgtca aacttgccgg tcgagtagta
      121 acccagaagg tagtaggcgc tcaggtcgtt tgtgatccgc tgcaacgcgg gagcgaagtt
      181 gttggtcgag accaccgcca cgccgtcggt gtattcggcc atcgtgcgta tcgatgtgtt
      241 gcgttcaccg agccggttgt tgtcctcgac gacggggatc atgggattct ggcccacgcc
      301 cgcaaccggc acgatgtcat cgtcgaatgg gacaaccccg cgcggatcga cgggatagaa
      361 ggagacgttt gcgcgattgg cgcgatcgag cagctgtcgg aaccgcatcg tgtgatcgag
      421 ttgcgccaga cgcattcgat cgccctcgca ggcgacgcca cccgcggcgg ccggcatcga
      481 caactggccg gtgcgcggat cgaagcctgg acgaggtatg ggtgaagcag cagggccgat
      541 gggacgcaca agagcctggt tcgggcggta caccctccag ccgtgcgtaa tcgtgatcac
      601 ggccctccgc tcttctctga tctcacgaag ctcggtcacg agcgcgtcga gcgcgtcgaa
      661 ggtctgcgcc tcccgacggc gaagaatcat ctcctgcgcg attcccaggt ctggcgcgac
      721 cgtctccccc ggtattcgcg gaagtcccgg atagcaggcc gcaaaccgct gctcgactgg
      781 atcagagagc agccggtcgc gcgctcccca gggccggctc aacgtgcctt caatcgcggc
      841 gacccgtcgc gtgaacgtga tcgcacgtac cggcgtcagc ggattcacga tcgcgacgag
      901 gtcgtccgga ccgatgaggc gacgcagcgc atcgatgagg ggcttggcga tcgtctgcga
      961 cgccgcgtaa tcaacgtgca tgacatcgag gaagatcacg aacacccggg cgccgggctc
     1021 ggcggcagct tgagcgctct cttccggaga ccggggatcg gttcgcgtcg tcggggcccc
     1081 tccggcctgg atgacgaccg gcgagaacga atctatcttc tgagggacgc catcctcgag
     1141 caggcggaac tcctcctggc gcaggtcgcc aatcgccgcg ccgtcacgcg tcgtggcgta
     1201 cgcgtctacg cggatgtaat tggcttccgt gcggaacgtc ggacgaggtt gatcctgggg
     1261 cggagcttgc tcggcggcac cggcgatcgc atacgcgatc aacatgccag ccacggcgag
     1321 aacacgccgc agatgtccgc ggacgaggtg tttcgacatc cccttccccc gctccgaggc
     1381 gctggcaggg gaagtctatc acctaatcgc cagggctcag gccgcgcgct ggtcggggac
     1441 ctccatcggc gaggggctct cgatggtgac cgactgaact ggcgacgtgg tgatctgcct
     1501 gccgcccaca ccgaattcca cgcgaagacc gacgagtatc cagccgagct tcagcgtgct
     1561 gccgccgaac gtggcgcccg agaggtgcac cacggttggc tcgagaaaga tcccgccctc
     1621 agccagcacc agacgccggg cgccgtcaag gacgc
//
LOCUS       DBNGSH010000396         2841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_715183, whole genome shotgun sequence.
ACCESSION   DBNGSH010000396 DBNGSH010000000
VERSION     DBNGSH010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_715183"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="AC16CY_09070"
     CDS             complement(<1..725)
                     /locus_tag="AC16CY_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3353979.1"
                     /translation="MSRIRTLIVDDEPIARERVLSLLQQEEDVEVVGQCGDGAQAVAA
                     IQHQLPDLVFLDVQMPGVDGFGVIEAIGPEKMPSVVFVTAYDEYALRAFEVHALDYLL
                     KPFGRDRFRQTLSHARAHLERRRAGDLGRRLLALVSDIKPEAPKLERLIVKSGGRVFF
                     LRTEDLDWIEAAGNYVRLHMGEESHLFRETMNRMESRLDPKRFVRIHRSRIVNTERIK
                     ELQPWLGGEHVVVLRNGARLPLSR"
     gene            complement(722..1840)
                     /locus_tag="AC16CY_09075"
     CDS             complement(722..1840)
                     /locus_tag="AC16CY_09075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3353980.1"
                     /translation="MGRRPPQIRLLHVFGVATVLGILTGLQAYNYVALFEERKQPFNV
                     LLLLNLTYWYSWAVLVPGILWVARQYRFGRQTWPRAALVHLLGVVGFTAAHVVLATSA
                     RGVILTQLTERSFDWLMYFQNRFLLNFDWEMMTYWALVGLSHALEYRRESQERELTAA
                     QLQMQLAEAQLEALQRQLHPHFLFNTLNTISALMHKDVHAADDMLVRLSDLLRLTLER
                     VGTQQVTLKDEIDFLRKYLEIEQTRFGDRLQVNIDVEPDALDALVPNLVLQPLVENAL
                     RHGIAPRISGGRIDVTARDAGDRLILCVRDDGVGLPADKMSDLHSGVGLSNTRSRLET
                     LYGDRHRFEFETPSGGGLVVTVEIPLVREPEDRMESVA"
     gene            2002..2808
                     /locus_tag="AC16CY_09080"
     CDS             2002..2808
                     /locus_tag="AC16CY_09080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3353981.1"
                     /translation="MGVVRIEAIDDERLSAFRGVPDPELLTRLGLFVAEGRLVVRRLL
                     ARSRLETRAVLVTEPALASIGDLVAGRPALPVFVVTQEVMNGVAGFNFHRGCLAIGVR
                     PPARHWREVADAARRIVVLERVGNADNVGAIFRNASAFGAGGVLLQSDCADPLYRKSI
                     RTSMAASLSMPFAAAEPWPAVLDALSGAGWTTVAMTPAADASVRAAAQRLGDSRIAIV
                     LGHEGEGLTPEAMAACSHRARIPMAAGIDSVNVATAAAIALYEMGSGVIY"
BASE COUNT          457 a          904 c         1002 g          478 t
ORIGIN      
        1 cggctgagcg gcagccgcgc gccgttgcgc agcacgacga cgtgctcgcc gccgagccac
       61 ggctgcagct ccttgatgcg ctcggtgttg acgatgcgcg agcggtgaat gcggacgaag
      121 cgcttcggat cgagccgcga ttccatgcgg ttcatcgtct cgcggaacag gtgcgactct
      181 tcgcccatgt ggagccgcac gtagttgccg gcggcctcga tccagtcgag gtcctcggtg
      241 cggaggaaga acacgcgtcc gccggacttg acgatcaggc gttcgagctt gggggcttcc
      301 ggcttgatgt cgctgacgag cgcgaggagg cggcggccca ggtcgccggc gcggcggcgc
      361 tccaggtgcg cccgcgcgtg cgagagcgtc tggcggaacc ggtcccgccc gaacggcttc
      421 agcaggtagt cgagggcgtg cacttcgaac gcgcgcaggg cgtactcgtc gtaggcggtg
      481 acgaacacga ctgacggcat cttctcgggg ccgatggcct cgatgacgcc gaagccgtcc
      541 acgccgggca tctgcacgtc gaggaacacg agatcgggca gttgatgctg gatggcggcg
      601 accgcctggg cgccgtcgcc gcactgaccg acgacctcga cgtcttcctc ctgctgcaaa
      661 agcgagagca cgcgctcccg cgcgatcggc tcatcgtcaa cgatcagggt tcggatgcga
      721 ctcatgccac gctctccatg cgatcttccg gctcgcgtac caacgggatc tcgaccgtga
      781 ctaccaagcc gccgccagag ggagtctcga actcgaatcg gtggcggtcg ccatagagag
      841 tctcgaggcg cgaacgcgta ttggagagcc caacgcctga gtgaaggtcg ctcatcttgt
      901 cggccggcag gccgacgccg tcgtcgcgca cgcacaggat caggcgatct ccggcgtcgc
      961 gggcggtgac atcgatgcgg ccgccgctga tcctcggcgc gatcccgtgc ctcagcgcgt
     1021 tctcgacgag tggctgaaga acgagattgg ggacgagcgc gtcgagcgcg tcgggctcga
     1081 cgtcgatgtt cacctggagc cgatccccga agcgggtctg ctcgatttcc aagtacttcc
     1141 ggagaaagtc gatttcgtcc ttgagcgtca cctgctgcgt gccgacgcgc tcgagcgtga
     1201 ggcgcagcag gtcgctcaac cgcaccagca tgtcgtcggc ggcatgcacg tccttgtgca
     1261 tgagcgccga gatggtgttg agcgtgttga agaggaagtg cgggtggagc tggcgctgca
     1321 gcgcctcgag ctgcgcttcg gcgagctgca tctggagctg cgccgcggtc agctcgcgct
     1381 cctgcgactc gcgacggtat tcgagcgcgt ggctgaggcc gaccagcgcc cagtaggtca
     1441 tcatctccca gtcgaagttc agcaggaacc tgttctggaa gtacatcagc cagtcgaagg
     1501 accgctcggt gagctgcgtc aggatgacgc cgcgagccga cgtggccagg accacgtggg
     1561 ccgccgtaaa ccccacgaca cccaggaggt ggaccagcgc ggcgcgcggc cacgtctgcc
     1621 gcccgaaccg gtactgccgc gccacccaga ggatgcccgg gacgagcacg gcccatgagt
     1681 accagtaggt caggttgagc aggaggagta cgttgaacgg ctgctttcgc tcctcgaaga
     1741 gcgcgacgta gttgtacgcc tgcagtcccg tcaggatgcc gagcaccgtg gcgacgccga
     1801 aaacgtgaag cagccggatc tggggggggc ggcggcccat ccatgcactt tgggccgggt
     1861 tacgcttcac gtcaagccac gctcatcacc agacgtgagt gtgtctgaca cgctcatcac
     1921 gcgtttcgtc accgagaacc agcgcttcgt cacgaatttc gtcccagtgc cgagccccgg
     1981 gcccgcgagt acgatgctcc cgtgggggtt gtacggatcg aggccatcga cgacgagcgg
     2041 ctatcggcgt tccgcggcgt gccggaccct gaattgctca cgcgtctggg tctgttcgtc
     2101 gccgaagggc ggctcgtcgt caggcgcctg ctcgcccgtt ctcgactcga gacgcgcgca
     2161 gtgctggtca ccgagccggc cctggcctcg atcggcgacc tcgtcgcggg ccgcccggcg
     2221 ctgcccgtct tcgtcgtgac gcaggaggtc atgaacggcg tggccgggtt caactttcac
     2281 cgggggtgcc ttgcgattgg cgtgcgtcct cctgccaggc actggcgcga ggtggcggac
     2341 gcggcacgcc ggatcgtggt gctcgagcgg gtgggcaacg ccgacaacgt gggggcgatc
     2401 ttccgcaacg cctccgcgtt cggcgcgggt ggcgttctgc tccaatcgga ctgcgccgat
     2461 cctctctacc gcaagtcgat caggacgtcg atggccgcct cgctctcgat gccgttcgcg
     2521 gcggccgagc cgtggccggc cgtgctggac gcgcttagcg gcgcgggatg gacaacggtc
     2581 gcgatgacgc cggctgccga cgcatcagta cgagcggcgg cacagaggct cggcgacagc
     2641 cggattgcga tcgtgctggg gcatgaaggt gaagggctga cgcccgaagc gatggccgcg
     2701 tgcagtcacc gcgcccggat cccgatggcc gcgggaatcg actcggtgaa cgtggcgacg
     2761 gccgcggcga tcgcgttgta tgaaatgggg tcgggagtca tttattgaaa agggggtcgg
     2821 gagtcatttt ccctggagga t
//
LOCUS       DBNGSH010000397         2106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2412863, whole genome shotgun sequence.
ACCESSION   DBNGSH010000397 DBNGSH010000000
VERSION     DBNGSH010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2412863"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..>2106)
                     /locus_tag="AC16CY_09085"
     CDS             complement(186..>2106)
                     /locus_tag="AC16CY_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3353982.1"
                     /translation="LVHLLQRCLVKDPRDRLQAIGDARIELDEISRTSSELRPSSPRR
                     NERSRVLAWSLAALLVCSAAGAAAFVWWPREVTAPSSISRWTIQLPEDQSIAAGRLPG
                     GLGIGRPSIALSPDDRYIVYVVERGAQTMLHVRATNEFEGKPIPGTEGSFQPFFSPDG
                     QWVGFFTDTKLKKVFLQGGSPVELCDAVHVYGASWGSDDRIYFADREANRLTRIAATG
                     GTPEVVANSSEVGTTLLWPHILPGSKAVLFNSPFPGTINVLSLEDRRRRPLIAGASAA
                     YAASGHIVFSRGSDVLAATFDLERLAITGPAVPVLENVRTEALDASQFSISTRGTLAY
                     VEGGFARYANLVWLDRQGRETPVDVPAQVYGSFTLSPDGRRVAVIIPAQAEGQTNDIW
                     IYDLERRNRRPLATRVQNNPVWAPDGRSVFLSLEQNSAVNIYRQTVDGTGVAERLTES
                     RYNQFLQTVSPDGKILVYGESTASGDRDFWSLRLDGSGKPQLYLQTPAREVFAAFSPD
                     GRWLAYVNDAAGRYEVYVVAADGTGDRRLVSTEGGEEPRWSARGDLFYRNGQQWMAVA
                     VTPGAPGAAFSAGRPQTLFEGPFPNIPGYSYDVTADGQRFLMAREAVTGRSREIRIVL
                     NWFEDLKQRVRAAGQ"
BASE COUNT          371 a          688 c          693 g          354 t
ORIGIN      
        1 cctgcagtac aacttcttga tccgcttcat cgtcaagcag atctcgaaga aggagggcgg
       61 tcccaccgat acgagccggg accacgagtt gaccgactgg gcgcaggtgg agaagctggc
      121 gcacgacctg gccacgaaga tccgggaacg cgttgccgcg gtcgcttgac gaggaaggtc
      181 cgaggctact ggccggccgc acgcacgcgt tgcttcagat cttcgaacca gttgaggacg
      241 atgcggattt ccctggagcg tcccgtgacc gcctcgcggg ccatcaggaa tcgctggccg
      301 tccgccgtga cgtcgtacga ataaccggga atgttcggaa acggtccttc gaataacgtc
      361 tggggccgac ccgcgctgaa ggcggcgccg ggcgccccgg gcgtgacggc gacggccatc
      421 cattgctggc cgttgcggta gaagagatcg ccccgcgccg accaacgcgg ttcttcgccg
      481 ccttccgtgg agaccagccg cctgtcgccc gttccgtccg cggcgacgac gtaaacctca
      541 taccggccgg cggcatcgtt gacataggcg agccaccggc cgtccggtga gaatgcggcg
      601 aagacctctc gcgccggtgt ctgcagatac agctggggct tcccgctgcc gtccagcctc
      661 agcgaccaga aatcgcgatc acccgacgcc gtgctttcac cgtagacgag gatcttgccg
      721 tccggcgaaa cggtctgcag aaactggttg taccggctct ctgtcagccg ttccgcaacg
      781 ccggtcccgt cgacggtttg ccggtagatg ttcaccgcac tgttctgctc cagactcaga
      841 aacacagacc tgccgtcagg agcccatacc gggttgttct ggacgcgcgt ggccagcggc
      901 ctccggttgc gtcgctcgag atcgtaaatc caaatgtcgt tggtctggcc ctccgcctgc
      961 gcgggaatga tgacggcgac gcgacgcccg tccggcgaga gtgtgaacga tccatagacc
     1021 tgggccggca cgtccaccgg agtctctcgc ccttgccgat cgagccacac gaggttggcg
     1081 tatcgcgcga aacctccctc gacatacgca agcgtacctc gggtcgagat cgagaactgg
     1141 ctggcgtcga gcgcttcggt gcggacgttc tccagcacgg gcaccgccgg tcctgtgatc
     1201 gccaggcgct cgagatcgaa cgtcgccgcc aggacgtcgc tcccgcgcga gaacacgatg
     1261 tggccgctcg cagcgtatgc ggcgctcgcg ccggcgatga gcgggcgacg gcgccggtcc
     1321 tccaatgaca ggacgttgat ggtcccggga aacggcgagt tgaacagaac cgccttcgaa
     1381 cccggcagga tatggggcca tagcagcgtc gtgcccacct cgctggagtt ggccaccacc
     1441 tcgggtgtcc cgccggtcgc agcgattctg gtgagccggt ttgcttcccg gtcggcaaag
     1501 tagatgcgat cgtcgctgcc ccagctggcg ccgtacacgt gaacggcgtc gcacaactcg
     1561 accggcgatc cgccttgcag gaacactttc ttgagcttgg tgtctgtgaa gaagccgacc
     1621 cattgcccgt cgggagaaaa gaacggctgg aagcttcctt ccgtcccggg aatcggtttg
     1681 ccctcgaact cattcgtcgc gcgcacgtgc agcatcgtct gtgcgccgcg ctcgacgacg
     1741 tagacgatgt agcggtcatc gggggagagg gcgatggagg gcctcccgat gccgagcccg
     1801 ccgggcagcc gtccagccgc gatgctctgg tcctcgggca gctgaatcgt ccatcggctg
     1861 attgacgatg gagcagtcac ctcgcgaggc caccagacga acgctgcagc gccggcggcc
     1921 gagcagacca gaagcgcggc gaggctccag gcgagcacgc gggaacgctc gttccgtcgc
     1981 ggcgacgacg gccggagctc ggacgacgtt ctcgagatct catcgagctc gatgcgggcg
     2041 tcgccaatgg cctgcaggcg atcccgcggg tctttgacga gacagcgttg caggaggtga
     2101 acgagc
//
LOCUS       DBNGSH010000398         3691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1055144, whole genome shotgun sequence.
ACCESSION   DBNGSH010000398 DBNGSH010000000
VERSION     DBNGSH010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1055144"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="AC16CY_09090"
     CDS             <1..540
                     /locus_tag="AC16CY_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR3353983.1"
                     /translation="QVRDVIISHLHYDHCGNHDLFPRARYHLQDREMAYGTGRFMCHA
                     HQRVPFEVDDVVAMVRKVFDGRVTFHDGDDELAPGITLHRIGGHSMGLQCVRVRTRRG
                     HVVLAADATHLYSHVNEGRVFPITYSVAETLEGYATLKKLADSPDHIVPGHDPEVLKR
                     YPAARRGLEDWVVDLAGSR"
     gene            569..2965
                     /locus_tag="AC16CY_09095"
     CDS             569..2965
                     /locus_tag="AC16CY_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR3353984.1"
                     /translation="MRTILCLVLLALSVTTAFAQDVTEYRRLRDARPDGRTIPVKGLT
                     IARDGYRIELRSGVVHLLAPSGADTFGAVFIGEGVYQLNPATAAERRHLSLVTGNDRL
                     EVLTDRFTRSIFLFTDRTAADLLAHAPATSGTPDPAATRVFDDYIQKQLTGAHPNLHL
                     RVLADLLNRPARTDGVFLAFVEGQAYSPVLLVVDPLGISNVTSRFAFFGGEEVALVSF
                     NNLDGGLWYSSAVAGQAVDGRGKAIRSLADATHYDIDTVLDGGALRGTTTITFTTTVE
                     GVRVLPIHLFERLRIRTAAVERDGTLVQAGVLQEEPAQGWAARLFGDEVPAGDVAILF
                     REPLARGATIRLRLEYEGRDVLQGSDGRFVVRARESWYPNLGTFSDLATYATTFRYSQ
                     RHQLVGVGELVSERVEGGQKVALWRSQVPIRVAGFNYGEFQKQSREDAESGVQVNVYA
                     IRGSENFTAMAGNALADAVNTSRVATLFFGAAPYRHLSITQQPQMNFGQSWPSLVFLP
                     SMSFVSATDVARNTSIDPRALQGLKEFGNTVAWHEVAHQWWGHQIGWSSYRDQWLSEG
                     LAEFTSALMLEVTAGRKAADGFWELRRGEVLDRGRGVPHTEAGAITQGFRLATSRSPG
                     AARAILYSKGAYVVHMLRMMMREDRVPDPDRVFKAMMHDFVTSWSGKNPSTDDFQAVA
                     ERHMTRGTNLAGDGKLTYFFNQWVHGTAVPTLSSDLQVTDLGGRKYRISGTITQAGVT
                     EGFRTPVPIYLDFGDEKIERLGLLPLSGPVTQKVSVEIELPQRPRRVVINARHDVLSR
                     "
     gene            complement(2955..3536)
                     /locus_tag="AC16CY_09100"
     CDS             complement(2955..3536)
                     /locus_tag="AC16CY_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3353985.1"
                     /translation="MHTRIASLDCRGGFPDADVDRLAAGDSVELTGPVLTFRDASAAR
                     IARALEAGEPPPVPLGGRILYAVGPSPAKPGQIIGSAGPTTTARMARYFPALFSAGVR
                     AIIGKGELHGEEMAAFVRHRAVYFAAIGGLGALLAKQITAATVVAYTELGAEALYELD
                     LDAFPAVVIIDRAGRNFHESARARWRKSPPATG"
BASE COUNT          619 a         1230 c         1233 g          609 t
ORIGIN      
        1 caggtgagag acgtcatcat cagccacctg cactacgacc attgcggcaa ccacgacctg
       61 tttccgcggg cgcgctacca cctccaggac cgcgagatgg cgtacggcac gggacgcttc
      121 atgtgccacg cgcatcagcg cgtcccgttc gaggtggacg acgtcgtggc gatggtgaga
      181 aaggtcttcg acggccgcgt cacgttccac gacggcgacg acgagctggc gcccggcatc
      241 acgctgcacc gcatcggcgg acactcgatg gggctgcagt gcgtgcgcgt caggacgcgc
      301 cgcggccacg tggtgctcgc cgccgacgca acgcacctgt attcgcacgt gaacgaggga
      361 cgcgtcttcc cgatcaccta cagcgtcgcg gagacgctcg aaggctacgc gacgctgaaa
      421 aagcttgcag actcgcccga tcacatcgtt cccgggcacg acccggaggt gctgaaacgg
      481 tatcctgccg cgcggcgcgg gctcgaggac tgggtcgtcg atctggctgg tagccggtag
      541 ctggtagctc cgtgttagca tccgcgccat gcggacaatc ctttgccttg ttctcctggc
      601 gctgtccgta acgacggctt tcgcccagga cgtcactgag tatcgccggc ttcgcgacgc
      661 gcggcccgac ggccggacga tccccgtgaa agggctgacg atcgcccggg acggctaccg
      721 aatcgagctc cgctccggtg tggtgcatct gctcgccccg tccggcgccg acacgttcgg
      781 ggcggtattc atcggcgagg gcgtctatca gctgaacccc gcgaccgccg cggagcgccg
      841 ccacctgtcc ctggtcaccg gcaacgacag gctcgaggtg ctgacggatc gattcacgcg
      901 gtccatcttc ctcttcacgg atcggacggc cgccgacctg ctggcgcacg cgccggcgac
      961 ctcagggaca ccggacccgg ccgccacgcg cgtcttcgac gactacatac agaagcagct
     1021 gacgggagcg catccgaacc tgcacctgcg tgtgcttgcc gacctcctca accggcccgc
     1081 gcgcaccgac ggcgtgtttc tggccttcgt cgagggccag gcatactcgc ccgtcctgct
     1141 ggtcgtggat ccgctcggga tttcgaacgt gacgtcgcgt tttgcattct tcggcggcga
     1201 agaggtcgcc ctcgtgtcgt tcaacaacct cgacggcggg ctgtggtact cgagcgcggt
     1261 cgcggggcag gccgtcgacg gacgcgggaa ggccattcgc agcctggccg acgcgacgca
     1321 ctacgacatc gacacggttc tcgatggcgg cgcgcttcga ggcacgacga cgattacctt
     1381 cacgaccacg gtcgagggag tccgggtgct cccgattcac ctgttcgagc gacttcggat
     1441 ccggacggcc gcggtcgaga gggacgggac gctggtccag gccggcgtcc tgcaggaaga
     1501 gcccgcgcag ggatgggcag cgcggctgtt cggcgacgag gtccctgcag gcgacgttgc
     1561 gatcttgttt cgcgagccgc ttgcgcgagg cgcgacgatc cggctcaggc tggagtacga
     1621 gggccgtgac gtgcttcagg gcagcgacgg ccgcttcgtg gtccgggcgc gagagagctg
     1681 gtatccgaac ctcgggacgt tctccgatct ggcaacctat gcgaccacgt tccgctattc
     1741 acaacggcac caactggtgg gcgtcggaga gctggtgagc gagcgggtcg agggcggcca
     1801 gaaggtcgca ctttggcgga gccaggtccc gatccgcgtg gcgggattca actacggtga
     1861 attccagaaa cagtcccggg aagacgccga gtctggggtg caggtcaacg tgtacgccat
     1921 ccgcgggtcc gagaacttca cggctatggc ggggaacgcc ctggccgatg cggtgaacac
     1981 gtcgcgcgtc gccacgctgt tctttggcgc cgctccctat cgtcacctgt ccattaccca
     2041 gcagccgcaa atgaacttcg gtcaatcgtg gccgtcgctc gtcttcctgc cgtcgatgtc
     2101 gttcgtctcg gcgaccgacg tggcgcggaa cacgagcatc gacccccggg cgctccaagg
     2161 cctcaaggag ttcggcaaca ccgtggcgtg gcacgaggtg gcccatcagt ggtggggcca
     2221 tcagatcggc tggtcgagct accgcgatca atggctgtca gagggcctgg ccgagttcac
     2281 ctcggccctg atgctcgaag tcaccgccgg gcgcaaggcc gctgacgggt tctgggagct
     2341 gcggcgcgga gaggtgctcg accgcggccg aggcgtgccc catacggaag cgggcgccat
     2401 cacgcaggga ttccgcctcg cgacgagccg ctcgcctgga gccgcccgcg cgatccttta
     2461 ctccaagggc gcctacgtcg tgcacatgct caggatgatg atgcgcgagg accgcgtccc
     2521 cgatccggat cgcgtgttca aggcgatgat gcacgacttc gtgacgagct ggtcggggaa
     2581 gaatccatcg accgacgact ttcaggcggt tgccgaacgg cacatgaccc ggggcacgaa
     2641 cctcgccgga gacggcaagc tgacctactt cttcaatcag tgggtgcacg gcacggcagt
     2701 gccgacgctg tcgtccgatc tgcaggtgac cgacctcggc gggcgcaagt atcggatctc
     2761 gggaacgatc acccaagccg gcgtgacgga gggatttcga acgccggtgc ccatctatct
     2821 ggatttcggc gacgagaaga tcgagaggct cggcctcctc ccgctctcgg gacccgtcac
     2881 ccagaaggtc tccgtcgaga tcgagctccc ccagcgccct cgccgcgtgg tgatcaacgc
     2941 gaggcacgac gtactcagcc ggtagctggt ggcgacttcc gccaacgtgc gcgggcgctc
     3001 tcgtgaaagt ttcgtccggc ccggtcgatg atgacgacgg ctggaaacgc gtcgagatcc
     3061 agctcataga gcgcttccgc gccgagttcc gtatacgcca cgacggtcgc tgccgtgatc
     3121 tgcttcgcca gcagcgcgcc gaggccgccg atcgcggcga agtacacagc gcgatggcga
     3181 acgaaggccg ccatctcctc gccgtggagc tcgcccttcc cgatgatcgc gcgcacgccg
     3241 gccgagaaca gcgccggaaa atagcgcgcc atgcgcgcgg tcgtcgtcgg cccggcggat
     3301 ccgatgatct gccctggttt cgccggagac ggcccgacgg cataaagaat gcgccccccg
     3361 agcgggacgg gcggcggctc gcccgcctcg agcgcgcggg cgatgcgcgc cgcgctggcg
     3421 tcgcgaaacg tgagcaccgg gccagtcagt tcgacggagt cgcccgccgc gagccggtct
     3481 acgtcggcgt caggaaaacc gccgcggcaa tcgaggctcg cgattcgagt gtgcatgaac
     3541 aaacctcacg ccgttaacca cagagacaca gagtttggaa tatctctgtg ttctccgcgc
     3601 ctctgtggtt gacgtacgtg atcctcatag ttctgccgtc atgcgccgcg tcgagtgaca
     3661 cccgaggttc accgccaccg ggagcccggc g
//
LOCUS       DBNGSH010000399         2827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2243593, whole genome shotgun sequence.
ACCESSION   DBNGSH010000399 DBNGSH010000000
VERSION     DBNGSH010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2243593"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..570
                     /locus_tag="AC16CY_09105"
     CDS             109..570
                     /locus_tag="AC16CY_09105"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3353986.1"
                     /translation="MRTDVQGPRLWAQGPGDRDAKFETQERRRAARQVPDPLAPIARV
                     RLRTGRELTVIDLSPLGVLVEGSTRLLPGTHLDVHVTSAQGRIPVRARVVRCHVWSLT
                     AELVQYRGAFAFGALVELALSSRSSVEGTTSDCETREADGSPHKLRPGSVR"
     gene            complement(839..2566)
                     /locus_tag="AC16CY_09110"
     CDS             complement(839..2566)
                     /locus_tag="AC16CY_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353987.1"
                     /translation="MLSPPPPAIWTVLLAGLTFAALVLIARRSRAFAAPSTYVVSGFS
                     RTVDVVSGFSRTRWAPLLAGLLTLIAVRIVWGSFSEPGVIHDERAYLLQASIFAQGQW
                     TLPSPPLPAFFEQMHVFVEPAVFAKYPPGHALVLAFGAWLGLPGLMPVLLAALSGGLI
                     FWIARRLANEWVSLFTWWLWTTGWANLYWAASYFSETTSTAMWWLAVFGTIRWLDSGR
                     TAYLTLAAAALAFGIETRPLTIVALSLPLAWVIVRRLKDVATAATTLALPAVAALAIL
                     ALGPIWNQQTLGDWRQNPYSYYSQAYFPFDKPGFGVDATPPLRQPQPELAAVGEWSRQ
                     VHSDYVPAALPVAFVKRILSVLFWTIDGWRLWLGVLILAAVLHGSRIERLGLAAAGTL
                     FLGYLSFAHPPMWIVYYVEILPILYFLAACQMGRLFHTLSGAGRGDGPVWPAHVTAAA
                     AVTALLLVPLGVSDVWRVRGAVDQRNAFHRSARAAVEKNTPVKSIVFVRYSADHNPHL
                     ALTGYGADLASTGRWVAVDRGDRNRELLSLAPGVPAFVLDTSTLRVAPLTNESNSRAD
                     NSVDEKTRR"
     gene            complement(2560..>2827)
                     /locus_tag="AC16CY_09115"
     CDS             complement(2560..>2827)
                     /locus_tag="AC16CY_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3353988.1"
                     /translation="GYFAFWENNPWNPATRLVMSRIPFDRDAKLISPPRARSLLARAG
                     FDVLRTDFLFFFPRLLAALRPLEIRLSRIPAGAQYLVLCRKLEC"
BASE COUNT          564 a          892 c          969 g          402 t
ORIGIN      
        1 gcgtctacgt acgcggacgg gacgggaccc agtgggtcgt gcgggttctc ggggtgacgg
       61 cgcgagcgcg cgtgctgcga tacgtgccgg cgacgggcgc atgggtcaat gcggactgac
      121 gtccagggcc caaggctctg ggctcagggc ccaggagacc gggacgcgaa attcgaaaca
      181 caagagcgac ggcgtgctgc gcgacaggtc ccggatccgc tcgcgccgat cgcgcgggtg
      241 cgtctgcgca ccgggcgcga actcactgtg atcgacttgt cgccgttagg cgtcctcgtg
      301 gaggggtcca cacggctcct gccaggaact cacctggacg tccacgtgac gagcgcgcag
      361 ggacggattc ccgtccgcgc gcgcgtcgtg cgatgccacg tctggagctt gactgcggag
      421 ttggttcagt acagaggcgc gtttgcgttc ggcgcgctcg tcgagcttgc gctctcgtca
      481 cgcagttcag tcgagggcac gacatctgat tgcgagacca gagaggcgga cggatcaccc
      541 cataaactgc gaccggggtc tgtccgatag ggaggtctga acgcgcttca cccctcgcgc
      601 tttttcgggt aactgcccat gcttggtgtc attctcacgc ccctagctct cgcactcgtc
      661 gttgccggct tgcagttcat ccgaaaccga cgccggcgcc gccgtcagtc gtgggagcat
      721 ctgcataaca tcggaccggt gagcggccaa tggctcgccg atcgccgacg taccggttag
      781 aggcgactcc gccaggcaac agttcgtgca ctcgaaggca aaagagcgtc gagggaattc
      841 atcggcgtgt cttttcgtcc acagagttgt ctgcgcggga gttcgactca ttcgtcagcg
      901 gcgccacgcg gagcgtcgat gtgtcgagca caaacgccgg cacgcccgga gcgagggaga
      961 gaagctcacg gttccggtcg cctcgatcga cggcaaccca gcgtccagta gaagcgaggt
     1021 ccgcgccgta gcccgttaac gccagatgcg ggttgtgatc ggcggagtag cggacgaaga
     1081 caatcgactt gacgggcgtg ttcttttcga cggcggcgcg cgccgagcga tgaaaggcgt
     1141 tgcgttgatc gactgcgccg cgaacacgcc agacgtccga gacgccaagc ggcacgagca
     1201 gcagggccgt aacggcggcg gcggccgtca catgagcggg ccacaccgga ccgtcgcctc
     1261 gacccgcgcc gctcagggtg tggaagagac ggcccatctg gcacgccgca aggaagtaca
     1321 agatcggcag tatctcgacg tagtacacga tccacatcgg cgggtgcgca aaggacagat
     1381 aaccgaggaa cagcgtcccg gccgcggcaa gtcccaggcg ttcaatccgc gagccgtgca
     1441 ggaccgccgc caggatcaac acacccagcc acaaccgcca gccgtcgatc gtccagaaca
     1501 gcaccgacag aatccgtttc acgaaggcta ccggcaatgc agcgggcacg taatcgctgt
     1561 gcacctgacg cgaccactcg ccaacggccg cgagctccgg ctgcggctgt cggagcggag
     1621 gcgtcgcgtc tacgccaaac ccaggcttgt cgaacggaaa gtacgcctgc gaatagtacg
     1681 agtacggatt ctgccgccag tctccgagcg tctgctgatt ccagatcggg ccgagcgcca
     1741 gaatcgcgag cgcggcgacc gccgggaggg cgagcgtcgt cgcggccgtc gcgacgtctt
     1801 tcaggcgtct gacgatgacc cacgccaagg ggagggaaag agccacgatc gtcagcggcc
     1861 tcgtctcgat gccgaacgcc agcgccgcag cggcgagcgt caggtacgcc gtgcgccccg
     1921 agtcgagcca gcgaatcgtg ccaaagacgg caagccacca catggcagtg ctcgtggttt
     1981 ccgagaaata cgaagcggcc cagtacaagt tggcccagcc cgttgtccac agccaccacg
     2041 tgaacagcga gacccactcg ttggccagcc gcctcgcgat ccagaaaatc agcccgccgg
     2101 aaagcgcggc cagcagcacc ggcatcaagc cggggaggcc gagccacgcg ccgaaagcaa
     2161 gcaccagcgc gtggccgggc ggatacttgg cgaacaccgc cggctcgacg aacacgtgca
     2221 tctgctcgaa aaaggccggc agaggaggag agggcagcgt ccattgtccc tgggcgaata
     2281 tcgaggcttg cagcagatag gcgcgctcgt cgtggatgac accgggctcg ctgaacgagc
     2341 cccagacgat ccggacggcg atcagtgtca gcagccccgc gaggagggga gcccaccggg
     2401 tccggctgaa gccggacact acgtcgacgg tccggctgaa gccggacact acgtacgtcg
     2461 atggcgcggc gaacgcccgg ctgcgacggg cgatgaggac aagggcggcg aacgtcagcc
     2521 ccgcgagaag aacggtccag atcgcgggcg gaggggggct cagcattcga gcttgcggca
     2581 gagcaccaga tactgcgcgc cagccggaat tcgagagagc ctgatctcca gcggcctcag
     2641 tgcggcaagc aggcggggga agaagaacag gaagtcggtg cgcagaacat caaagcctgc
     2701 ccgggcgagc aggctgcggg cacggggagg cgagatgagc ttcgcgtcgc ggtcgaacgg
     2761 aatgcggctc atgacgagcc tcgtcgcagg gttccagggg ttgttctccc agaaggcgaa
     2821 gtatccg
//
LOCUS       DBNGSH010000400         2366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2583009, whole genome shotgun sequence.
ACCESSION   DBNGSH010000400 DBNGSH010000000
VERSION     DBNGSH010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2583009"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="AC16CY_09120"
     CDS             <1..191
                     /locus_tag="AC16CY_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3353989.1"
                     /translation="IEWTVTVSDPSTWTKPWTFSMPLTMNDAEPVLEFACHEGNYAVR
                     HILSGARAAEHRSESAKP"
     gene            complement(245..1123)
                     /locus_tag="AC16CY_09125"
     CDS             complement(245..1123)
                     /locus_tag="AC16CY_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate:acetyl-CoA acetyltransferase"
                     /protein_id="HIR3353990.1"
                     /translation="MHAQGQPCIITVAITGSVPRKENNPAVPITVSEQIESTHAAFEA
                     GAAVVHVHVRNDDQSTTSNPDRFADFQERIRPLCPGIVVQFSTGGRSGVGSERGGMLH
                     HRPDMASLATGSCNFPTRVYENSPDLIRELAEKMNQYGVKPEIEVFDLAMLYNAVDYA
                     RSGLLKAPLHMQFVFGVKHALPAARDILEFEVRKLGELMPEATWVAAGIGRDQLEVNR
                     WCLEMGGHCRTGLEDNVRWDKNRLAASNAELVSRVAGLCADYDRPVATPATARAILGL
                     NRTPDTRVRHTESPRH"
     gene            1218..>2366
                     /locus_tag="AC16CY_09130"
     CDS             1218..>2366
                     /locus_tag="AC16CY_09130"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3353991.1"
                     /translation="MCRLVVVAACLLGGWACSSSTPAPPAESGPAVTVFEGARLIVGD
                     GSDPIERSAFIVQDRSVVQAGRQGELQVPNGAKRVDLTGKTVMPALIDAHSHLGYTNV
                     KERTTSAANYTRENLVDHLRRYAFYGIAATLSMGVDRGELAYELRATAVPGAALLLTA
                     GRGIALPNAGPGAEYRRDAAYGVSNEAEARSAVRELAAKKVDFVKIWVDDRDGTVAKL
                     PPRLYRAIIDEAHKNSLRVVAHIFDLADAKALVRSGIDGFAHGVRDVDIDQEFIDLIK
                     GRPEVFVIPNLPDRDTSDDYAWLSDTVPAQEIQRLRDARAKRTPAAAKQAQTLFEVQA
                     RNLARLNAAGVKIAFGTDAGVSVGWVAHTELADMVAAGMTPAQVLTAAT"
BASE COUNT          399 a          771 c          812 g          384 t
ORIGIN      
        1 ggatcgagtg gaccgtcacc gtgagcgacc cgtcgacctg gacgaagccc tggacgttct
       61 cgatgcctct cacgatgaac gatgccgagc cggtcctcga attcgcctgc cacgaaggca
      121 actacgcggt gcgccacatc ctcagcggtg cccgcgccgc cgagcatcgc tctgaatccg
      181 caaagccgta gtgagcgatt acgacacacc gagacagtga ggcaccgaga aagactctcc
      241 agactcagtg cctcggtgac tcggtgtgac gtactcgagt atccggagtt cggttcaggc
      301 caagaattgc tcgcgcggtg gcaggcgtgg ccaccgggcg gtcgtaatcg gcgcagagcc
      361 ccgcgacgcg cgagaccagc tccgcgttcg acgcggccag gcggttcttg tcccagcgca
      421 cgttgtcttc gagccccgta cggcagtgtc cgcccatctc gaggcaccag cgattgacct
      481 cgagctggtc ccggccgatt ccggccgcca cccaggtggc ctccggcatc agctcgccga
      541 gcttgcgcac ctcgaactcg aggatgtcgc gcgcagccgg cagcgcatgc ttcacaccga
      601 acacgaactg catgtgcagc ggcgccttca gcaggcccga tcgcgcgtag tccaccgcgt
      661 tgtacagcat ggccaggtcg aagacctcga tttccggctt cacgccgtac tggttcatct
      721 tctctgccag ctcgcggatc agatccggcg agttctcgta gacgcgcgtc ggaaaattac
      781 aagagccggt ggcaagcgac gccatgtcgg gccggtgatg cagcatgccc ccgcgctcgg
      841 atccgacgcc cgatcgcccg cccgtcgaga actggacgac gatgcccggg cagagcgggc
      901 ggatgcgctc ctggaagtcg gcgaacctgt cggggttcga cgtggttgac tgatcgtcgt
      961 tgcgcacgtg cacgtgcacg acggcggcgc ccgcctcgaa ggcggcatgc gtggactcga
     1021 tctgctcgct gacggtgatc ggcacggccg ggttgttctc cttgcgcggt accgagccgg
     1081 tgatggcgac ggtgatgatg cagggctgcc cctgggcgtg cacgggatat gtataaccta
     1141 tcaacggtgc gaaggtgcga aggtgcgagg gtgcgaaggt gcgagggtgc tgaatcgggg
     1201 ctagaattca ctgccgtatg tgtcgtctcg ttgttgtcgc agcgtgtctg ctcggcggct
     1261 gggcctgttc ctcgtccact cccgcgccgc ccgcggaatc ggggccggcg gtcaccgtgt
     1321 tcgaaggtgc gcgcctgatt gttggcgatg gcagcgatcc gatcgagcgc tcggccttca
     1381 tcgtgcagga ccgatccgtc gtgcaggcgg gacggcaggg cgagctgcag gtgcccaacg
     1441 gcgcgaagcg ggttgacctg accggaaaga cggtgatgcc ggcgctcatc gacgcgcaca
     1501 gccatctcgg ctacaccaac gtgaaggaga ggaccacctc ggccgccaac tacacgcgcg
     1561 agaacctcgt cgaccatctc cgtcgctacg ccttctacgg catcgccgcc acgttgagca
     1621 tgggcgtcga tcgcggcgag ctggcctacg agttgcgcgc gacggccgtg cccggtgccg
     1681 cgctcctgct caccgccggc cggggcatcg cgctcccgaa tgccggcccg ggtgcggagt
     1741 accgtcgtga tgccgcgtac ggcgtgtcga acgaggccga ggcgcgctcg gcggtccggg
     1801 agctggccgc gaagaaggtc gacttcgtga agatctgggt cgacgaccgc gacggcaccg
     1861 tcgccaagct gccgccgcgc ctctaccggg ccatcatcga cgaggcgcac aagaacagcc
     1921 ttcgagtcgt cgcccacatc ttcgacctgg ccgacgcgaa ggcgcttgtg cgatcgggga
     1981 tcgacggctt cgcgcacggt gtgcgcgacg tggacatcga ccaggagttc atcgatctga
     2041 tcaagggcag gccagaggtg ttcgtgattc cgaacctgcc cgacagggac acgagcgacg
     2101 actacgcctg gttgagcgac acggttcccg ctcaggagat tcagcgcctg agagacgcgc
     2161 gggcgaagcg gaccccggcg gccgcgaagc aggcgcagac gctgttcgag gtgcaggcgc
     2221 gcaacctggc caggctcaac gcggccggcg tgaagatcgc cttcggcacg gatgcgggag
     2281 tatcggtcgg ctgggtggcg cacaccgagc tcgccgacat ggtggccgcg gggatgacgc
     2341 cggctcaggt gctcacggca gccacg
//
LOCUS       DBNGSH010000401        16189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1055458, whole genome shotgun sequence.
ACCESSION   DBNGSH010000401 DBNGSH010000000
VERSION     DBNGSH010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 16189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16189
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1055458"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /locus_tag="AC16CY_09135"
     CDS             complement(<1..2177)
                     /locus_tag="AC16CY_09135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF039586.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3353992.1"
                     /translation="MSLSLRQGSAPARPAAQPSRRSPAPASRRPFLDNTPLLLVGVMA
                     LVAALAGLLALGRGSSRLAPDFLTDVVLSALSATNVTILVALAFVLTRNIFKLVVERR
                     RALPFARFRAKLVAVLLGMAVVPAVLVLIVGSELIRTSVDRWFNQPVDEVLSSANSIA
                     ADYYAQQRSLVSTQAQRLARALSGVDLASAGPSNVRDLVAPDVLQERIDLVEVYRVGR
                     GSDGQVKVTPVVDVASPSMPREYSRASADRLAEQAAGAASERPLVDPLAGGGELIRIA
                     MPIRATPAGPVTGVVVASDYLTDRFAARARGMTEAFEAYQQLRVLKQPLAGVYLSFFL
                     TLTLMILVGATWMGLYLAKRITRPVQMLATAADEIGAGHLDHRVKAEAGDEFGSLIDA
                     FNRMAGDLAANRRRLERSSVELERKHRDVEGRRRYVETILDRLGTGVVSVDATGSIRT
                     WNAAASRLLGVDQRVSGLPVSTVLGASELKPLAIAIDEAARRREDLPPQEVSITREGR
                     DVHLAVMTTPLKRDDGTADGVVVVFDDVSPLIRVQKVAAWREVARRLAHEIKNPLTPI
                     QLSAERLRRHFTGAPDATRELVAECTTTIVGEVESLKGLVDEFSQFARMPAPRAVPTD
                     VHGLLDEALSLYRGLLAEVEIRTKYDGSLPRVLLDAEQFRRVILNLVDNAIEAMDRRG
                     VIDIETWHERNDNLVRIVVADNGPGIPLAERDKLFLPYYSTKKR"
     gene            complement(2234..2692)
                     /gene="nrdR"
                     /locus_tag="AC16CY_09140"
     CDS             complement(2234..2692)
                     /gene="nrdR"
                     /locus_tag="AC16CY_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR3353993.1"
                     /translation="MKCPYCGHLGDKVVDSRESKEGDVIRRRRECLDCGRRFTSYERI
                     DEIPYMVIKKDGTRERFERQKLIAGLLKACEKRPVSIAAIEAVADKAEATLQERPEKE
                     MATEEVGALVMDELKRLDKVAYVRFASVYRHFRDIGEFMTELKDLLNAKD"
     gene            complement(2689..3717)
                     /locus_tag="AC16CY_09145"
     CDS             complement(2689..3717)
                     /locus_tag="AC16CY_09145"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3353994.1"
                     /translation="MNRRTIIVSLLTLGLMAWFLRGANLGAVWLQVRQVRGDLLLVSM
                     AAMAFSFVARTIRWRYLLAPIGPTQFRTVFRTTVIGFGALGLLPVRVGDLVRPYLLAR
                     QERLPVSSALATVVMERVLDLIAVLALLAIYVWGFGVDSSLPPRLLRPIEVSAAVAAV
                     LSAAGLAIMWVLATHPERVGVLASAFARVLPGRLSGRVGRLASTFSSGFAAARDPRAL
                     VMALVWSFPVWLAISAQVWAVTLAFEIDMPYAGTFLLQSLLVIGVAMPTPGGVGSFHE
                     AYRIGVTTFFSASNDRAIAAAIVAHAISFVPPVLVGIVFMAQDGLSIGGLKDLASTAR
                     AREGSDKS"
     gene            3849..4790
                     /locus_tag="AC16CY_09150"
     CDS             3849..4790
                     /locus_tag="AC16CY_09150"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR3353995.1"
                     /translation="MPVSCAVFRNKATTLPTTAPLVETRLNGLGPHRQGKVRDIFDLG
                     DTLLLVATDRISAFDYVLGSGIPDKGKVLTQLSAFWFGQTGDIVPNHLVTMDVQAYPG
                     DLRKHADVLSGRSMLVQKTTPVLIECVARGYLSGSGWKEYQASGSVCGVALPKGLRES
                     DRLPEPIFTPATKADSGHDVNISEEEAGRLIGVDLTRRLKDLTLRMYAHGVKHAEARG
                     IILADTKFEFGIVGQGKVILIDEVLTPDSSRYWPKDQYAPGGPQPSFDKQYVRDYLEE
                     IRWNKQPPVPSLPDDVVMRTREKYLEAFHRLTGKALL"
     gene            4819..5037
                     /locus_tag="AC16CY_09155"
     CDS             4819..5037
                     /locus_tag="AC16CY_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3353996.1"
                     /translation="MTGLKEQLERLIDDMVTRGVSFEDARREFEKKFISLVLLKSDGN
                     LCQAADVLGMHRNTLSRKIAEYRLRRTA"
     gene            5195..5488
                     /gene="groES"
                     /locus_tag="AC16CY_09160"
     CDS             5195..5488
                     /gene="groES"
                     /locus_tag="AC16CY_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3353997.1"
                     /translation="MKVRPLHDRIIVQRIEEGEQKIGGIIIPDSAKEKPQQGKVIAVG
                     LGKVKEDNTRQPLDVKDGDTILFGKYSGQEIKIDGEDYLIMREEEVLAVLASK"
     gene            5566..7203
                     /gene="groL"
                     /locus_tag="AC16CY_09165"
     CDS             5566..7203
                     /gene="groL"
                     /locus_tag="AC16CY_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786183.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3353998.1"
                     /translation="MAKQIIYGEQSRQAILRGVNQLADAVKVTLGPKGRNVVLDKKFG
                     SPTITKDGVTVAKEIDLKDPLENMGAQMVREVASKTSDTAGDGTTTATVLAQAIYREG
                     ARNVVAGANPMELKRGIEKAVEVLVEDIKKMSRPVSGNMVAQVGTISANSDVTIGKII
                     AEAMDKVGKDGVITVEEARTLETSLDVVEGMQFDRGYLSAYFVTDPERMEVVLENPVI
                     LIHEKKISSMKDLLPVLEQVARLSRPLLIIAEDIEGEALATLVVNKLRGTLQAAAVKA
                     PGFGDRRKAMLEDIAILTGGRAITEDLGIKLEGIKIDDLGRAKKITIDKDNTTIVEGA
                     GTSSAIEGRVKQIRAQIEDTTSDYDREKLQERLAKLVGGVAVIKVGAATETEMKEKKA
                     RVEDAMHATKAAVEEGIVPGGGVALLRAGKALDKLKLDGDQAVGLQIIKRAIEEPMRW
                     IAMNAGQEGSIVVSKVKEMKNNDDGFNAATETYEDLVKAGVIDPAKVVRSALQNASSI
                     ASLLLTTEALVSEIPEEKKEPSGGHGHGGPGGMGGMY"
     gene            complement(7284..7859)
                     /locus_tag="AC16CY_09170"
     CDS             complement(7284..7859)
                     /locus_tag="AC16CY_09170"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3353999.1"
                     /translation="MSATPSTMLALGTRMPAFRLPDVNGAIVSSDACAGRPVLVAFIC
                     RHCPFVRHIRREFARFTSEFLARGLPTIAINSNDVEAFPEDGPEGMKQEAGEAGYTFP
                     YLYDETQEVAKVFKAACTPDLFLFDASGSLVYRGQFDSSRPRSDVPVTGADIRAAASA
                     LLENRPIPPDQRPSVGCNIKWKPGNEPDYWK"
     gene            complement(7867..8718)
                     /locus_tag="AC16CY_09175"
     CDS             complement(7867..8718)
                     /locus_tag="AC16CY_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3354000.1"
                     /translation="MAESTQQMKNEPEPGERATGHLRVRPDNFGPVELRFQFEQPRVG
                     VALGAGFLVYAVVIGIALLIMLLVPKEVIQAVVPKFENVIWLPDPGPGGGGGGGNKQP
                     DPPKPAEIVKPKPAPEPEPVQIPEEPPPIAPIIPLQTLEAVTTAPGSLESTQVSNSLG
                     SGTGAGAGPGKGDGLGPGEGGGIGGGVHRPGNGVSVPTLVKKVDPQYTADAMRAKVQG
                     VVILECVVMPDGTVGKVDVMRSLDATFGLDQEAIKAAKQWRFKPGTRQGEPVAVLVTI
                     ELTFTLR"
     gene            8938..9099
                     /gene="tatA"
                     /locus_tag="AC16CY_09180"
     CDS             8938..9099
                     /gene="tatA"
                     /locus_tag="AC16CY_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR3354001.1"
                     /translation="MPNIGIPELLIILAIVILVFGASRLPEIGRGIGKGIRNFKEATK
                     EGSEGAEKP"
     gene            complement(9096..11366)
                     /gene="rnr"
                     /locus_tag="AC16CY_09185"
     CDS             complement(9096..11366)
                     /gene="rnr"
                     /locus_tag="AC16CY_09185"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR3354002.1"
                     /translation="MSVSDNLLSLIRQHVHHPATARELARVLRVPREELPGFKRRLKT
                     LVASGDLLQIRGNRYGLPEKMDLVVGRLTTNPGGFGFVIPEQAGQAEGGDIYIAAANL
                     TEAMHGDRVVARVERQTDRGPEGRIVRVLERSQARIVGRFDVDAAGLGYVVPFDRRVL
                     TDVRVPTGQSSSAEAGDIVVTEITTWPTATRGPVGRVVEVLGRIDEPGVDTQIIIRKF
                     NIPDHHSEEAVAEAVRLGSVVKPRDIEGRTDFRNTTTVTIDGEHARDFDDAITIARLD
                     NGHYWLGVHIADVSHYVTEGSALEEEAYDRGTSVYFTERAVHMFPAELATGLCSLNPH
                     VDRLVQSCLMEVDAHGAVVRYEMHDGVINSDERMTYTAVNAVLTERDPEAIRQYGPLV
                     PMFELMRELFEILLQRRRRRGSIDFDLPEAEVVLSEIGEIEAIIAAERNVAHRIIEEF
                     MLLANETVASHLEQNGVPALHRVHEPPDAKKVEDFEAFITTLGYSLAARGRALTPKHF
                     QKLVERIHGTPEERPIAALMLRTMQKARYDAASLGHFGLAAEHYAHFTSPIRRYPDLV
                     VHRMLRESRRGPIPAGRLEELNEDLPEAARHASEMERRAAEAERELLQWKKVRFMSGK
                     VGDEYEGYITGVAPFGLFVELVEHYVEGLVHVSSMADDYYRFVEQEHTLRGEHTKKVY
                     RLGDRVGVQVVRVDLERRQIDLGLVDVLDAVRRETRGTRPIKSRVKPKKERRKEQRPG
                     RRERAARSSGRRGRRR"
     gene            complement(11370..12776)
                     /locus_tag="AC16CY_09190"
     CDS             complement(11370..12776)
                     /locus_tag="AC16CY_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR3354003.1"
                     /translation="MRRRARRPGREPRPPSVRSRGRAFCLGRSAASELTSVLDLREHL
                     RIIVGEAIAAVNAGGLVARVLGGDMADTLRSARAVDVVAAGKASAAMLDAFAAAAPVP
                     LRRVLAIGPRSPTLPPLPPGAEWCGAGHPLPDDNSLAGARRALEIARGGGPGDALVVL
                     LSGGGSALMALPAPGLTLEAKQHTARTLMQQGADIYELNTVRKHLSAIKGGRLAAAAG
                     GSVLTLAVSDVVGDDLSVIASGPTCADDTTYDDALAVLSRRGGPSAYREEVVTHLERG
                     RKGEIPETPKTGDARLERAAASVIGPQRGAIEGARRAAATLGYHVHVVNEPITGEARV
                     AGRAHIDAVSRVRSSLPRPLCVISSGETTVTVVGKGTGGRNQEFALAMAQSLEAVGPS
                     VVAASIGTDGIDGPTDAAGAVVDSTTLSRALRSGLAPETYFADNNTYAFFDRLGDLIR
                     TGPTNTNVGDLQVILVGG"
     gene            complement(12674..12985)
                     /locus_tag="AC16CY_09195"
     CDS             complement(12674..12985)
                     /locus_tag="AC16CY_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354004.1"
                     /translation="MPALRNRLSRFLTDSPAACDPVLHTLSNFYREEFVRCQRCLDAQ
                     REHYSASAIGDVERALTQVMSQLDRLCAEERADQVVSHVLRQFDLVAGLSAWADPRQV
                     N"
     gene            complement(13028..13939)
                     /locus_tag="AC16CY_09200"
     CDS             complement(13028..13939)
                     /locus_tag="AC16CY_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR3354005.1"
                     /translation="MRAIEYAFRQGAASLWRSRGASAFAVLAIALALVVLGSLLLLTW
                     NAEQVLSRWTAAAEFSVYLHDQATSEQRGAIESLIDRSGVSAGREYVSKSQALARFRR
                     EFTELAALAEGFEDNPFPASIEVRIGPAGDEAGRVDTLVREMVALPGVVDVRYDRDWL
                     ARIGSGLQGFRAAGLALAVLMAVAAAVTVAAVVRLGLFARSEELEIMELVGAPLAYIR
                     GPFVAEGFIQGGIGALLALAGLWGGLLVANAWWGAELAAALDGGTLRFLPWALCAYLI
                     AGGMFVGSAGGLVAAWRAGAGAGPGPR"
     gene            complement(13936..14595)
                     /gene="ftsE"
                     /locus_tag="AC16CY_09205"
     CDS             complement(13936..14595)
                     /gene="ftsE"
                     /locus_tag="AC16CY_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR3354006.1"
                     /translation="MIETHHLSKVYSRGLYALRDLTLTVDKAEFVFLTGPSGAGKSTF
                     LRLLLLQERPTEGQVFVNGHNLAVLSRREIQEYRRGIGFVFQDFKLIQSRTILENISF
                     VPEVLGVPAAQQRRRAFQVLKWVGLQHRMNAYPKDLSGGEQQRIAIARALVNDPALLI
                     ADEPTGNLDPDLSLEIMNLLREINAGGTTVLVATHDRELIRLVGRRTITLDQGRVVEV
                     A"
     gene            complement(14704..16146)
                     /gene="gatB"
                     /locus_tag="AC16CY_09210"
     CDS             complement(14704..16146)
                     /gene="gatB"
                     /locus_tag="AC16CY_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3354007.1"
                     /translation="MTDFEPVIGLEIHVQLGTRTKMFCSCELSFGAEPNTRTCPVCLG
                     LPGTLPVVNAQAIHYGLLMGMAFGCEIAPRSIFHRKNYFYPDLPKGYQISQYELPLAL
                     GGGLEITTEGSTKRVGLTRIHMEEDAGKSLHEGFSDSDKRSYVDYNRSGVPLIEIVSE
                     PDMRSAAEAAEFFSRLRDLLVWLGVSDGNMEEGSLRCDANVSVRPLGQSALGTKAEVK
                     NVNSFRYLEKALEYEIARQIELVESGGSVVQETRLWDTGTGRTYSMRSKEEAHDYRYF
                     PEPDLPPLVIDASRVESVKADMPELPEARSRRLAAEYGIPAYDAGVLTQSAALADYFE
                     ATARAAGNAKAASNWIMGELLRTMKERGQAIADVALTPAALGELIVLIDKGTISSSMA
                     KGVFAKMYDSGRSATSIVEEEGLAQIADEGAIAAIVADVLRGQAEAVAQFRAGKRQTF
                     GFLVGQVMKASGGQANPKLASDLLKRALDE"
BASE COUNT         2834 a         5507 c         5285 g         2563 t
ORIGIN      
        1 cgcttcttcg tcgagtagta cggcaggaac agcttgtcgc gctcggcgag cggaatgccc
       61 gggccgttgt ccgccacgac gatgcgaacc aggttgtcgt tgcgctcgtg ccacgtctcg
      121 atgtcgatga cgccccgccg gtccatcgct tcgatggcat tgtccacgag gttcaggatg
      181 acgcggcgga attgctcggc gtcgagcagc acccgtggca gcgatccgtc gtacttcgtg
      241 cgaatctcga cctccgccag cagaccgcga tagagcgaga gtgcctcgtc cagcaggccg
      301 tgtacatccg tcggcacggc gcgcggcgcc ggcatgcggg caaactgcga aaactcgtcc
      361 acgaggccct tcaacgactc gacctcgccg acgatggtgg tggtgcactc cgcgacgagc
      421 tcccgcgtcg cgtccggggc cccggtgaag tgacggcgca ggcgctccgc gctcagttga
      481 atgggcgtca gcggattctt gatctcgtgc gccagccggc gcgccacttc ccgccatgcc
      541 gccaccttct gcacgcgaat cagcggcgac acgtcgtcga agacgacgac gacaccgtcg
      601 gcggtcccgt cgtcgcgctt caagggagtc gtcatgacgg cgaggtgaac atcgcgtccc
      661 tcacgcgtga tcgacacttc ctgcgggggc aggtcctccc tgcggcgcgc ggcttcgtcg
      721 atggcgatgg ccagcggctt cagctccgag gcgccgagta cggtggagac cgggaggccc
      781 gacacgcgct gatccacgcc gagcagccgc gaggcggcgg cgttccacgt gcggatgctg
      841 cctgtcgcgt ccaccgacac cactccggtc ccgagccggt cgaggatggt ttcgacatag
      901 cgccggcgcc cctcgacgtc ccggtgcttc cgctcgagct ccaccgaaga ccgctcgagc
      961 cggcgtcggt tggccgccag gtctccggcc atgcggttga aggcgtcgat caaggagccg
     1021 aactcgtcgc cggcttcggc cttcacccga tggtcgagat gtccggcgcc gatctcgtcc
     1081 gccgccgtgg cgagcatctg caccggccgc gtgatgcgct tggccaggta aagccccatc
     1141 cacgtcgcgc ctacgagaat catcagcgtg agcgtcaaga agaaggacag gtacacgccc
     1201 gccagcggct gcttcagcac gcggagctgc tgataggcct cgaacgcctc ggtcatgccg
     1261 cgcgcgcgcg ccgcgaagcg atccgtcagg tagtcgctcg ccaccacgac cccggtgacg
     1321 ggaccggccg gcgtggcgcg gattggcatg gcgatgcgga tcagctcgcc gccgcccgct
     1381 aacggatcga cgagcgggcg ctccgacgcg gcaccggcgg cctgttcagc gagccggtcg
     1441 gccgaggcgc gcgagtactc cctcggcatc gacggcgagg cgacatcgac gaccggtgtc
     1501 accttgacct gtccatccga accccggcca acccggtaca cttcgaccag atcgatccgc
     1561 tcctgcagga cgtccggcgc aacgaggtcg cgcacgttcg atggtcctgc tgatgccaga
     1621 tcgacgccgc tgagcgcgcg cgccagcctc tgcgcctgtg tcgagaccag gcttctctgc
     1681 tgcgcgtagt agtcggcggc gatcgagttg gccgacgaga gcacctcgtc gaccggctgg
     1741 ttgaaccagc gatccacgct cgtgcgaatc agctcgctgc cgacgatcag caccagcacg
     1801 gcgggcacga cggccatgcc gagcagcacg gcgaccagct tcgcccggaa gcgcgcgaac
     1861 ggcagcgcac gccgtcgttc gacgacgagc ttgaagatgt tccgcgtcag cacgaacgca
     1921 agcgccacga gaatcgtcac gttcgtggcg gagagggccg acaggacgac gtcagtcagg
     1981 aaatccggtg ccagccgcga cgagcctcgt ccgagcgcca gcaggcccgc gagggccgcg
     2041 acgagcgcca tgacgcccac cagcaacagc ggcgtgttgt cgagaaacgg cctgcgtgag
     2101 gccggggcag ggcttcgccg cgacggctgc gccgccggcc tggctggcgc cgacccctgt
     2161 cgcagtgaaa gtgacaccgc cgccctacag cctacagcct actgcccccg gtcccctgcc
     2221 ccctgccccc tgctcagtcc ttggcattca gcagatcctt gagctccgtc atgaactcgc
     2281 cgatatcgcg gaaatggcgg tacacggagg caaaccggac gtaggcgacc ttgtcgagcc
     2341 gcttcagttc gtccatcacc agggcgccga cttcttcggt ggccatttcc ttctcgggtc
     2401 gttcctgcag tgtggcctcg gccttgtcgg caacagcctc gatggcggcg atgctcaccg
     2461 gccgcttctc gcatgccttc agcagcccgg cgatcagctt ctgccgctcg aaacgctcgc
     2521 gggtcccgtc tttcttgatc accatgtacg ggatctcgtc aatccgttcg tagctggtga
     2581 agcgccggcc gcagtcgagg cattcccggc gccggcggat cacgtcgccc tccttgctct
     2641 cgcgggagtc gacgaccttg tcacccagat gaccgcagta cgggcacttc atgacttgtc
     2701 ggacccttcc ctggccctgg cggtgctggc cagatccttc agtccgccga tgctcagccc
     2761 gtcttgagcc atgaacacga tgcccacgag caccggcggg acgaacgaga tggcgtgagc
     2821 gacaatcgcg gcggcaatgg cgcggtcgtt ggacgcgctg aagaaggtcg tgacgccgat
     2881 gcgataggcc tcgtgaaagc tgccgacgcc gccaggcgtc ggcatggcca cgccgatgac
     2941 gagcagtgat tgcaagagga acgtccccgc gtacggcatg tcgatctcga acgcgagcgt
     3001 caccgcccag acctgggccg agatcgccag ccagaccgga aatgaccaca cgagagccat
     3061 cacgagcgcg cgtgggtcgc gcgccgcggc aaagccactg ctgaacgtac ttgcaagacg
     3121 gcccacacgc ccggagagcc gccccggcag cacgcgcgcg aacgccgacg cgaggacccc
     3181 cacccgttcc ggatgagtgg ccaggaccca catgatggca agccccgccg ccgaaagcac
     3241 ggccgccacg gccgccgaga cctcgatggg acgcaggagc cgcgggggca ggctgctgtc
     3301 cacgccaaac ccccatacgt agatggcgag cagcgcgagc acggcaatca aatcgagcac
     3361 gcgctccatc acgaccgtcg ccagagcgga tgagaccgga agacgctcct gtcgagcgag
     3421 caggtacggg cggacgaggt cgcccacgcg aaccggcaac aggcccaacg caccgaatcc
     3481 gatgaccgtg gtacggaaga cggtgcggaa ctgcgtcgga ccgatcgggg caagcaggta
     3541 tcgccagcgg atcgtccgcg ccacgaacga aaaggccatc gctgccatcg acacgagcag
     3601 cagatcgccg cgcacctgtc gaacctggag ccagacagcg ccgaggttgg ccccgcgaag
     3661 gaaccacgcc atcagcccca acgtcagcag cgaaacgatg atggtacggc ggttcacgcg
     3721 ttcagggggt tcagggggtc cagaggattc agcgcttacg cgcttcgccg ccacaatata
     3781 tcaggctctc cgtcgcgcga ggcctgcgct gttgcgtaca gttgcagccg gcagcgccgc
     3841 tgggtaagat gccggtttcc tgcgcagtct tccggaacaa ggccaccacg ttgccaacca
     3901 ccgctcccct cgttgaaacg cgcctgaatg gactcggccc tcatcgccag ggcaaggttc
     3961 gcgacatctt cgatctcggc gacacgctgc tgctcgtcgc gaccgatcgc atctcggcct
     4021 tcgactacgt ccttggctcc ggcatacccg acaagggcaa ggtgctcacg caactctcgg
     4081 cgttctggtt cggccagacc ggcgacatcg tccccaatca cctcgtcacg atggacgtgc
     4141 aggcgtaccc cggcgatctt cgcaagcacg cggatgtgct gtcgggacgg tcgatgctcg
     4201 tccagaagac gacgcccgtg ctcatcgagt gtgtggcgcg gggctatctg tccggctccg
     4261 gctggaagga ataccaggcg agcggctcgg tctgcggagt ggcgctcccg aaggggctgc
     4321 gagaatcgga ccgtctgccg gagccgatct tcacgcccgc cacgaaggcc gacagcggcc
     4381 acgacgtcaa catcagcgaa gaggaagcgg gacgcctcat cggagtcgat ctgacgagac
     4441 ggctgaagga cctcacgctc cggatgtacg cgcacggcgt caagcacgca gaagcgcgcg
     4501 gcatcatcct cgctgacacc aagttcgaat tcgggatcgt cggccagggc aaggtcatcc
     4561 tgatcgacga ggtgctgacg cccgactcgt cccggtactg gccgaaggac cagtacgctc
     4621 cgggcgggcc tcaacccagc ttcgacaagc aatacgtccg cgactacctc gaggagattc
     4681 gctggaacaa gcaacccccc gtgccctccc tcccggatga cgtggtgatg cgtactcggg
     4741 agaagtacct cgaagcgttt caccggctca ccgggaaggc ccttctctga ccggccgccg
     4801 cccgctcgga ggcttcgcgt gacagggttg aaggaacaac tcgagcgcct gatcgacgac
     4861 atggtcaccc gcggcgtgtc gttcgaggac gcccggcgcg agttcgaaaa gaagttcatc
     4921 tcgctcgtcc tgctcaagtc ggatggcaac ctgtgccagg cggccgatgt cctgggcatg
     4981 caccgcaata ccctgagccg caagatcgcc gagtatcgac tgcggcggac cgcctgacca
     5041 ccggcccttg acagggtttt tcgggcttca ctacaattgg cagtcgcgcg ggttgagtgc
     5101 caacacgcag tgcccgcgcc agtgaaatca cgaggtttgc ccacggggct cccgccccac
     5161 ggcaccacta ccagacagct ggagtcgaag acacatgaaa gtcagaccgc tacacgaccg
     5221 catcatcgtt cagcgaatcg aggaaggcga acagaagatt ggcggaatca tcatccccga
     5281 cagcgccaag gaaaagccgc agcaggggaa ggtgatcgcc gtcggtctcg gcaaggtgaa
     5341 ggaagacaac acccgtcagc ctctcgacgt caaggacggc gacaccatcc tgttcggcaa
     5401 gtactccggt caggaaatca agattgacgg cgaggactac ctcatcatgc gcgaagagga
     5461 agtgctcgca gtgctcgcct ccaagtagcg cgggggcctt cagccccccg cgggggtgtt
     5521 tgcctgcgcg gaagcaaccc gcgcagccga tggagtgaga ggaacatggc aaagcagatc
     5581 atttacggcg agcagtcgcg gcaagccatc ctgcgcggcg tcaaccagct cgccgacgcg
     5641 gtgaaggtca cgctcggtcc caagggacgc aacgtcgtcc tcgacaagaa gttcggctcc
     5701 cccaccatca cgaaggacgg cgtcacggtg gccaaggaga tcgacctcaa ggacccgctc
     5761 gagaacatgg gcgcgcagat ggtgcgcgag gttgccagca agacgtcgga cacggctggc
     5821 gacggcacga cgacggcgac ggtgctggcc caggcgatct accgagaagg cgccaggaac
     5881 gtcgtggcgg gcgccaaccc gatggagctc aagcgcggca tcgagaaggc ggtcgaggtc
     5941 ctcgtcgagg acatcaagaa gatgtcacgg ccggtctcgg ggaacatggt ggcccaggtc
     6001 ggcacgatct ccgcgaacag cgacgtgacc atcggcaaga tcatcgcgga agcgatggac
     6061 aaggtcggca aggacggcgt catcacggtc gaggaagctc gaacgctcga gacctcgctc
     6121 gacgtcgtcg agggcatgca gttcgaccgg gggtatctct cggcctactt cgtgaccgac
     6181 ccggagcgca tggaagtcgt cctcgagaac ccggtgatcc tgatccacga gaagaagatc
     6241 tcgtcgatga aggacctcct cccggttctg gaacaggtcg cccggctgag ccggcccctg
     6301 ctcatcattg ccgaggacat cgagggcgag gcgctggcca ccctcgtcgt caacaagctg
     6361 cgcgggacgc tgcaggcggc tgccgtcaag gctcccggct tcggcgatcg ccgcaaggcg
     6421 atgctcgagg acatcgcgat cctcaccggc gggcgcgcca tcaccgagga tctcgggatc
     6481 aagctcgaag gcatcaagat cgacgacctc ggcagggcga agaagatcac gatcgacaag
     6541 gacaacacga cgatcgtcga gggcgccggc acgtcgagcg cgatcgaggg ccgcgtgaag
     6601 cagatccgtg cgcagatcga agacacgacg tcggactacg atcgcgagaa gttgcaggag
     6661 cggctcgcga agctcgtggg cggcgtggcg gtcatcaagg tcggcgcggc gaccgagacc
     6721 gagatgaagg agaagaaggc acgcgtcgag gacgcgatgc acgccaccaa ggcggcggtc
     6781 gaggagggca tcgtcccggg cggcggcgtg gcgttgctgc gcgcgggcaa ggcgctcgac
     6841 aagctgaagc tcgatggcga ccaggcggtg ggactgcaga tcatcaagcg ggccatcgag
     6901 gagccgatgc gatggatcgc gatgaacgcc ggccaggagg gctccatcgt cgtctcgaag
     6961 gtcaaggaaa tgaagaacaa cgatgacggc ttcaacgccg ccaccgagac ctacgaggat
     7021 ctggtcaagg cgggagtcat cgatcccgcc aaggtcgtgc gaagcgcgct gcagaacgcc
     7081 tcgtcgatcg catccctgct cctgacgacc gaagcgctcg tctcggagat cccggaagag
     7141 aagaaggaac cctccggcgg tcacgggcac ggcgggcccg gcggaatggg cgggatgtac
     7201 tgaggtcgaa actcaagact caaaactcag tcgaaactca agactcaaaa ctcaaagggc
     7261 gcgtccgcaa gggcgcgccc tttttatttc cagtagtccg gctcgttgcc gggcttccac
     7321 ttgatgttgc agccgacact cggccgctgg tccggcggga ttggccggtt ctcgagcagt
     7381 gcgctcgcgg cggcgcggat gtccgccccg gtgacgggca catcgctccg cggccggctg
     7441 ctgtcgaatt gtccccggta gacgagcgat ccgctcgcgt cgaagaggaa caagtcgggc
     7501 gtgcaggccg ccttgaatac cttcgcgacc tcctgggttt cgtcgtagag atacgggaac
     7561 gtgtagcctg cctcccccgc ctcctgcttc atcccctcgg ggccgtcctc gggaaacgcc
     7621 tcgacgtcgt ttgaattgat cgcgatcgtg ggaaggccgc gcgcgagaaa ctcgctcgtg
     7681 aaccgcgcga actcgcggcg gatgtgccgc acgaagggac agtgacggca gatgaaggcg
     7741 acgagcacgg gacggcccgc gcaggcgtcg gacgacacga tggcgccgtt cacgtcgggc
     7801 aggcggaacg ccggcatcct cgtgcccagc gcgagcattg tcgaaggggt ggcgctcatg
     7861 cgcgcctcac cgcagcgtga aggtcaattc aatcgtgacc aggacggcca cgggctcgcc
     7921 ttgccgcgtt ccaggcttga agcgccactg cttcgccgcc ttgatggctt cctgatcgag
     7981 cccgaacgtc gcgtcgagcg agcgcatgac gtcgaccttg ccgaccgtgc cgtcgggcat
     8041 cacaacgcac tcgagaatca ccacaccctg cacttttgcg cgcatggcgt cggccgtgta
     8101 ctgcgggtcg actttcttta cgagcgttgg cacggacaca ccgttgcccg gacgatgcac
     8161 gccgccgccg attccgccgc cctctccggg gccgaggccg tcgccttttc ccggtcccgc
     8221 cccggcgccg gtaccggagc ccagcgagtt ggacacctgc gtggactcga ggctgccagg
     8281 cgccgtggtc acggcttcga gcgtttgcag aggaatgatc ggtgcaatcg gcggaggctc
     8341 ctcggggatc tgcaccggtt caggctcggg agccggcttc ggcttgacga tttcagcagg
     8401 tttgggggga tcgggctgct tgttgccgcc gccgccgccg ccgcccggtc cgggatcggg
     8461 cagccagatg acgttttcga acttcgggac aacggcttga atgacttcct tggggaccag
     8521 aagcattatc agcagggcga tgccaatgac gaccgcgtac acgaggaacc cggcgccgag
     8581 cgcgacgccg acccgaggct gctcgaactg gaagcggagc tcgaccggcc cgaagttgtc
     8641 cgggcgcacc ctcaggtgcc cggtggcacg ctcgccgggt tcgggttcgt ttttcatctg
     8701 ctgcgtcgat tccgccatgg ccgcagtgtc gagatctatt gtccacgagc cgcgtcagct
     8761 gccgcaaggg cggcaatacg tcccatgtcg gctgacacgc gactgcacgc ccacacttcc
     8821 ttagacgctg ccgcagtact ccggtcttac gggagaaccg cgtcaggtgt ttctcgggcc
     8881 cttccggccc ccgtccttgc accgtccagc gtggtacaat ccgaccgaaa gggaaatatg
     8941 ccaaacattg gaattccgga gcttctcatc atcctggcca tcgtgatcct ggtctttggc
     9001 gccagccgac tgccggagat cgggcgcggg atcgggaagg gcattcggaa cttcaaggaa
     9061 gcgaccaagg aagggagcga gggcgccgaa aagccctagc ggcgccgtcc gcggcgcccg
     9121 gatgaccgtg ccgcccgctc gcggcggccc ggccgttgct ccttccgtcg ctcctttttc
     9181 ggcttgaccc ggctcttgat gggccgtgtt cctctcgttt cacgccgcac cgcatcgagc
     9241 acgtccacga gccccaggtc gatctgacgc cgctccagat cgacgcgcac gacctgtacg
     9301 cccacgcgat cccccagccg atacaccttc ttcgtgtgct ctccccggag cgtgtgctcc
     9361 tgttccacga accgatagta gtcgtccgcc atgctcgaca cgtgcaccag gccctcgacg
     9421 tagtgctcga cgagctcgac gaacaacccg aacggggcaa cgccggtgat gtagccctcg
     9481 tattcgtctc cgaccttgcc gctcatgaaa cgcaccttct tccactgaag gagctcgcgc
     9541 tcggcctcgg cggcccgccg ctccatctcg gatgcatggc gcgcggcttc aggaagatcc
     9601 tcgttcagct cttcgaggcg gccggcggga atcggaccgc gacgcgactc gcgaagcatc
     9661 cggtgcacga ccaggtcggg gtaccggcgg atgggcgacg tgaagtgcgc gtaatgctcc
     9721 gccgccagcc cgaagtgccc gaggctcgcc gcgtcgtacc gcgccttctg catcgtccgg
     9781 agcatcaacg ccgcaatcgg ccgctcctcg ggcgtgccgt gaatccgttc gacgagtttc
     9841 tggaagtgct ttggcgtgag cgcgcggccc cgggccgcca ggctgtagcc cagcgtggtg
     9901 atgaaggcct cgaaatcctc gaccttcttg gcgtcgggcg gctcgtgcac ccggtgcagc
     9961 gccggaacac cgttctgctc gagatgtgag gcgacggttt cgttggccag cagcatgaac
    10021 tcttcgatga tccggtgcgc gacgtttcgt tccgcggcga tgatggcttc gatctcgcca
    10081 atctccgaca gcaccacctc ggcctccggc aggtcgaaat cgatcgaccc gcggcggcgg
    10141 cggcgctgca gcaggatctc gaacaactcg cgcatcagct cgaacatcgg cacgagcggg
    10201 ccgtattggc ggatcgcctc cggatcgcgc tcggtcagaa ccgcgttgac ggccgtgtag
    10261 gtcatgcgct cgtcgctgtt gatcaccccg tcgtgcatct cgtatcgcac aacggcccca
    10321 tgcgcgtcga cttccatgag gcacgattgc accagccggt caacgtgcgg attgaggctg
    10381 cagagcccgg tggcgagctc tgccggaaac atgtgcaccg cccgctcggt gaagtacacc
    10441 gacgtgccgc ggtcgtacgc ctcttcctcg agcgcgctcc cctcggtcac gtagtgagag
    10501 acgtcggcga tgtggacgcc gagccaatag tgtccgttgt cgaggcgcgc gatcgtgatc
    10561 gcgtcgtcga agtcgcgcgc gtgctcgccg tcgatcgtca ccgtcgtcgt gttgcggaaa
    10621 tcggtgcggc cctcgatgtc ccgcggcttc acgacactcc cgagccgcac cgcctcggcg
    10681 accgcctctt ccgagtggtg atcggggatg ttgaacttcc ggatgatgat ctgcgtgtcc
    10741 acgccgggtt cgtcgatgcg gccgagcacc tcgacgacgc gaccgaccgg cccgcgcgtg
    10801 gccgtcggcc aggtcgtgat ctcggtcacc acgatgtcgc ccgcctcggc agacgaggac
    10861 tgccccgtcg gcacgcgcac gtcggtcagg acacgacggt cgaacggcac cacgtagccg
    10921 aggcccgccg cgtccacgtc gaaccggccg acgatgcgag cctggctgcg ctcgaggacg
    10981 cggacgatgc gcccctcggg gccgcggtcg gtctgtcgct ccacgcgggc aacgacgcgg
    11041 tcgccgtgca tcgcctccgt caggttggcc gccgcaatgt agatatcgcc tccctcggct
    11101 tgaccagctt gctcgggaat cacgaacccg aacccgcccg ggttggtcgt caggcggccc
    11161 acgacgaggt ccatcttttc cggcagcccg tagcgattcc cacgaatctg caggagatcc
    11221 cccgaggcga ccagcgtctt cagccggcgc ttgaagccgg gcagctcttc gcgcggcacg
    11281 cgcagcaccc gggccagttc gcgcgccgtc gccggatggt ggacgtgctg ccggatcagt
    11341 gagaggagat tgtcggaaac ggacactact tatccaccaa ccagtatgac ctgcaggtcg
    11401 ccgacgttgg tgttggtcgg cccggtgcga atgaggtcgc ccagcctgtc gaaaaacgcg
    11461 taggtattgt tgtcggcgaa gtacgtctcg ggagccaacc ccgatcgcag cgcgcgggac
    11521 agagtcgtcg aatcgaccac ggccccggcc gcgtcggttg gtccgtcgat gccgtcggta
    11581 ccgatgctgg ccgccacgac gctcggtccg acggcctcga gcgactgtgc catcgccagc
    11641 gcgaactcct gattcctgcc gccagtgccc ttccccacga cggtgaccgt cgtctcacct
    11701 gaagagatga cgcacagtgg ccggggcagc gacgaccgca cgcgagacac ggcgtcgatg
    11761 tgcgctcgtc cagccacccg ggcttcgccg gtgatcggct cgttgacgac gtgcacgtgg
    11821 tagccaagcg tcgccgccgc gcgtcgcgcg ccctcgatgg cgccacgctg gggaccgatg
    11881 acggatgcgg ccgcccgctc gagccgggcg tcgccggtct tgggcgtttc agggatctca
    11941 cccttccggc ctcgttcgag atgcgtcacg acctcctcgc gatacgccga agggccgcca
    12001 cgccgcgaca gcaccgccag cgcgtcgtcg taggtggtgt cgtcggcaca ggtcggccca
    12061 gaggcgatca ccgagaggtc gtcgcccacg acgtcggaaa cggcgagcgt gagcaccgac
    12121 ccaccggcag ccgcggccag gcgtccgccc ttgatcgcgg acaggtgctt ccgcacggtg
    12181 ttcagttcgt agatgtcggc gccctgctgc atcagcgtcc gcgccgtgtg ctgcttggcc
    12241 tcgagcgtga gcccgggcgc cggcagcgcc atcagtgcag agcctcctcc cgacaacagg
    12301 acgaccagcg catcacccgg gccgccgccg cgggcaatct cgagcgcacg gcgcgcaccg
    12361 gcgaggctgt tgtcgtcggg cagcgggtgt cctgcgccgc accactccgc gcccggcggc
    12421 agcggcggaa gtgttggcga tctcggaccg atggcgagca ccctgcgcag cgggacgggc
    12481 gcggccgcgg cgaaggcgtc cagcatggca gcagacgcct tgcccgccgc gacgacgtcc
    12541 acggcgcgag cgctcctcag cgtatctgcc atatcaccac cgagcacgcg cgcgacgaga
    12601 cctccggcgt tcacagccgc gatggcttcg ccgacgatga tgcgaaggtg ttccctgaga
    12661 tcgaggactg aagtcagttc acttgccgcg gatcggccca ggcagaaagc ccggccacga
    12721 gatcgaactg acggaggacg tggctcacga cctggtcggc gcgctcttcg gcgcacaggc
    12781 gatccagttg cgacatcacc tgtgtcaagg cgcgctccac gtcgccgatc gcgctcgcgg
    12841 agtagtgctc gcgctgcgcg tcgaggcagc gttgacaacg aacgaactct tctcggtaga
    12901 aattggagag ggtgtgaaga accggatcgc aggcggcagg cgagtcggtc aagaatcggg
    12961 acaggcggtt cctcagcgct ggcatatccc ccgtccaatc aagtctagcc agccccgcaa
    13021 gctcgtgtca acgcggcccg ggtccggcac ctgcccccgc cctccaggcg gccaccaggc
    13081 cgccggcact gccgacgaac atccccccgg caatcaagta ggcgcacagc gcccagggga
    13141 ggaaacgaag ggtgccgcca tcgagcgcgg ccgccagttc ggcaccccac caggcgttgg
    13201 ccaccagcag accgccccag aggccggcga gggcaaggag ggcgccgatc ccgccctgaa
    13261 tgaagccctc ggccacgaag ggtccacgga tgtaggcgag cggcgccccg accagttcca
    13321 tgatttcgag ctcctcgctc cgggcgaaga gtccgagccg gaccaccgcc gcgacggtga
    13381 ccgcggcagc cacggccatc aggactgcca gggcaaggcc cgccgcccgg aagccctgaa
    13441 ggccgctccc gattctggcc agccagtccc ggtcgtaccg gacgtccacg acgccgggca
    13501 gcgccaccat ctcacgcacc agcgtatcga cgcggccggc ctcgtcgccg gccgggccga
    13561 tccggacttc gatcgacgcg ggaaacgggt tgtcctcgaa gccctccgcc agcgccgcca
    13621 gctcggtgaa ctcgcgacgg aagcgtgcga gcgcctggga tttggagacg tactcgcggc
    13681 cggccgagac gccgctccga tcgatcaacg actcgatcgc gccgcgctgc tcggaggtgg
    13741 cctgatcgtg caggtagacc gagaactcgg ccgccgcggt ccaacgcgac agcacctgct
    13801 cggcgttcca ggtgagcagc agcagcgatc cgagcaccac gagcgccagg gcgatcgcga
    13861 gaaccgcgaa cgcgctcgcg ccccggctgc gccagaggct ggccgcgccc tgccggaagg
    13921 catactcgat tgctctcatg ccacctcgac cacacgcccc tggtcgagcg tgatcgtgcg
    13981 gcgccccacc aggcgaatca actctcggtc gtgggtggcg accaggacgg tggtgccgcc
    14041 ggcattgatc tccctcagga gattcatgat ctcgagcgac agatcggggt cgaggttgcc
    14101 tgtcggctca tcggcaatga gcagcgccgg atcgttgacg agcgcgcggg cgatcgcaat
    14161 acgctgctgt tcgccccctg acaggtcctt gggatacgcg ttcatcctgt gctgcaggcc
    14221 cacccacttg agcacctgga acgcgcgccg gcgctgctgg gccgcgggaa cgccgagcac
    14281 ctcgggcacg aaggagatgt tctcgagaat cgtccggctc tgtatcagct tgaagtcctg
    14341 gaagacgaac ccgatgccgc gccggtactc ctggatctcg cggcgcgaga gcaccgcgag
    14401 gttgtggccg ttgacgaaga cctggccctc agtgggacgc tcctgcagga gcagaagacg
    14461 gaggaacgta gacttgcccg cgccgctcgg tccggtcagg aacacgaact cggccttgtc
    14521 caccgtgagc gtcaggtcgc gcagcgcgta gagtccgcgg ctgtagacct tcgacaggtg
    14581 gtgcgtctcg atcacgccaa tacactaccc gtacttggcg gggtcagatc ttgatttttg
    14641 cacgcgaagc ggcgttcgtc ccgccgcggt gcaaaaatcc gatctgaccc cggcgtcgac
    14701 tgtctactcg tcgagcgccc ttttcaacag atcggacgcc agcttcgggt tggcctggcc
    14761 gccgctggct ttcatgacct ggccgacgag gaagccgaac gtctgacgct ttcccgcgcg
    14821 gaactgggcg acggcctcgg cctggcctcg cagcacatcg gcaacgatgg cggcgatggc
    14881 gccttcatcg gcgatttgcg cgagcccctc ttcctcgacg atcgacgtgg ccgaccgtcc
    14941 cgagtcgtac atcttcgcga agacgccctt ggccatggag ctgctgatcg ttcccttgtc
    15001 gatcagcaca atcagctcgc cgagcgcggc cggagtcagc gccacgtccg cgattgcctg
    15061 cccgcgttcc ttcatcgtcc gcagcagctc gcccatgatc cagttgctgg ccgccttggc
    15121 gttgcccgcg gccctggccg tcgcctcgaa gtagtcggcc agcgccgccg actgggtcag
    15181 cacgccggcg tcgtacgccg gaatgccgta ctccgcggcg agcctccggc tccgggcttc
    15241 gggcagctcg ggcatgtcgg ccttcacgct ctcgacccgc gacgcgtcga tcacgagcgg
    15301 tggaagatcg ggctcgggga agtaccggta gtcgtgcgcc tcttccttgc tgcgcatcga
    15361 gtaggtccgc ccggtacccg tgtcccagag gcgcgtctcc tgaaccacgc tgccgccgct
    15421 ctcgacgagc tcgatctgcc gcgcgatctc gtattcgagc gccttctcga ggtaccggaa
    15481 ggagttcacg ttcttgactt ctgccttcgt gccaagcgcg ctctggccca gcggccgcac
    15541 cgacacgttg gcgtcgcagc ggagactgcc ctcctccatg ttgccgtcgc tgacgccgag
    15601 ccacaccagc aggtcacgca ggcggctgaa gaactcggcg gcctcggcgg cggatcgcat
    15661 gtcgggctcg ctcacgatct cgatgagcgg cacaccgctc ctgttgtagt cgacgtagct
    15721 gcgcttgtcg gaatccgaga agccctcgtg cagcgactta ccggcgtcct cctccatgtg
    15781 gatcctcgtg aggccgaccc gtttcgtact gccctcggtc gtgatctcca gcccgccgcc
    15841 cagtgcgagc ggcagctcgt actgcgagat ctggtagccc ttgggcaggt cgggatagaa
    15901 gtagttcttg cggtggaaga tcgaccgtgg cgcgatctcg cagccgaacg ccatccccat
    15961 cagcagcccg tagtggatcg cctgtgcgtt gaccacgggc agcgtgcccg gcaggccgag
    16021 gcagaccggg caggtgcggg tgttcggctc ggcgccgaag ctcagctcgc acgagcagaa
    16081 catcttcgtc cgcgtcccga gctggacgtg gatctcgagg ccgatgacgg gttcgaagtc
    16141 ggtcacgcga gcgcctccgt cccgccgaag tcgatcgcct tctcgagcg
//
LOCUS       DBNGSH010000402         7817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_36607, whole genome shotgun sequence.
ACCESSION   DBNGSH010000402 DBNGSH010000000
VERSION     DBNGSH010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_36607"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC16CY_09215"
     CDS             <1..197
                     /locus_tag="AC16CY_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018938438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HIR3354008.1"
                     /translation="NDLGPHDDGLVGAMHRAAAAIAKEHGHAEDGYRTVFNCNRAAGQ
                     TVFHIHLHLLAGRGLAWPPG"
     gene            257..469
                     /locus_tag="AC16CY_09220"
     CDS             257..469
                     /locus_tag="AC16CY_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354009.1"
                     /translation="MERRSKRRLLLVVVALGAVVSAVAYWSTRSSCIEAGPGLRGEVQ
                     VLAGKTLYFNGECWTTEPMPPRDMSL"
     gene            complement(627..1316)
                     /locus_tag="AC16CY_09225"
     CDS             complement(627..1316)
                     /locus_tag="AC16CY_09225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3354010.1"
                     /translation="MSRFRAAAVFFDVDFTLIYPGPTFQGEGYARFCERYGMTIDAAK
                     FDEAVRAASSVLDAAQDHVYDADIFVCYTKRIIEEMGGAGPRLDECASEIYREWAACQ
                     HFELYDDVAPVLRELAGRGLKIGLISNSHRCLASFQEHFELRGVITAAVSSSEHGYMK
                     PHPSIFEAALALAGVEARESVMVGDSLTHDIDGARRVGMRGVLVHRSSDPAPAADVPV
                     IRSMAELPALL"
     gene            complement(1313..2611)
                     /locus_tag="AC16CY_09230"
     CDS             complement(1313..2611)
                     /locus_tag="AC16CY_09230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3354011.1"
                     /translation="MIQAHAESHTSGPGLAPARAVLANGVTVIAKETRTTPAVTLHAN
                     IRAGTDCDPAEGEGLAHFMSRAIDRGTERCSADEIAERFDGRGVTLATTVGRHVLTLV
                     CTCLVEDFEDVLGMVAEIVMRPTFPPLEVDRRRGEIVTLIRQDADNPAVVAVETLNEM
                     LYPGGHPYGRRVRGTIDNVERIDRAALLEFHAAHCVPSALSLAFVGDIETERAIDAAR
                     AAFDGWTAGAAAPVTRQPAPAPGARRARVVPMMNKSQADIAYGFVSIARSDPAYYAYW
                     LMNNILGQYSLGGRLGDRIREQQGMAYYVFSSLEASLMPGPLLIRAGVSPSNVERAVQ
                     SIDAELARLAAEGPTEQETTESKQYLIGSLPRTLETNVGIASYLQSAEFFGLGLDYDV
                     RVPGLLRAVSRQDVHEAARRAIDPARATVVVAGPYEGSLA"
     gene            complement(2608..3930)
                     /locus_tag="AC16CY_09235"
     CDS             complement(2608..3930)
                     /locus_tag="AC16CY_09235"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3354012.1"
                     /translation="MATLEHASENASAVREEVLDNGLRVLIQPVHTAPLATVWCWYRV
                     GSKDEPPGLTGVSHWVEHMNFKGTTNIPREQVKGIIEKYGGYWNGYTWIDQTTYTETA
                     TRDALDRMLFIEAERMQGCLYDPADCESERTVIISELQGGENDPEQLLDQELTATAFR
                     AHGYRHPTIGWLSDLQTMTRDDLYGHYRRFYVPNNATLVVVGDVEAANVMDAVVRHFG
                     AMKPADVGRRQRTLEPQQLGERRLTIAKKGTTAYLRIGVHAPAATDPDFFALLVLDAV
                     LTGAKGLNIMASFRTPPPQRSTRLYQALVASGLASAVNGALAPTAEPFLYTISATATD
                     GTTLDRLEEAALTELDRVRTGGVTPQELAKAKNQLRARLVFENDSITNIAHQLGFFET
                     IASWRWLLSLGQALESVTADQVDAVAAERLNPAGRTIGRFEPAKDSGL"
     gene            4129..6900
                     /gene="ileS"
                     /locus_tag="AC16CY_09240"
     CDS             4129..6900
                     /gene="ileS"
                     /locus_tag="AC16CY_09240"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR3354013.1"
                     /translation="MKASLQTAEPEALARWKAMDLYGLIREKRRGAPKFVLHDGPPYA
                     NGQIHLGTALNKVLKDLVVKSRTMAGFDAPYVPGYDCHGLPIELRVERELGAKKRDLS
                     MADFRRACRAYAERFIGVMTSEFQRLMVFGDWDHPYVTMSFQYQADIARVLGKLVERG
                     LVYKGKKPVHWCIHCRTALAEAEVQYEDHSSPSIFVEFPLNPESRPELGSRFPALAGR
                     EVSVLIWTTTPWTIPSNLAVAFHPEFDYAAYDVDGRAVIVAEGLAESVGRIVGRPFDR
                     PVARMKGAALEGIRFRHPLYDRDSVGVLADYVTLEQGTGAVHTAPGHGADDFLTGMKY
                     GLEIYAPVGPGGHFLDTVQLFGGQRVFDANPNVERALEARGRLWHREMVSHQYPHCWR
                     CHNPVIFLATSQWFVRMDGEASINGGDGSLRTLRDAARHAIDHEVRWLPSWGRDRIFN
                     MVTNRPDWCISRQRAWGVPIPAVDCVKCGEAILTTQLIARAVSVFEQYSADAWYERPI
                     EEFLPPGLVCPSCGGTSFERERDILDVWFDSGSSHEAVLPRYPELTWPSDMYLEGSDQ
                     HRGWFQSSLLVALATRGRPAYRQVLTHGFLIDLEGQKMSKSRGNAMVPQEVIKDSGAE
                     IIRLWVAMTEFTEELRVSKEILTRVVDVYRKLRNTCRILIGNLYDFDPATDLVPLAQL
                     DEVDRYALARYGEAAERMLRAYDEYDFSTVSQALNTLATVDLSAFYVDVTKDRMYTLG
                     ARSRERRSTQTAMYLISEGLARLMAPILPATADDLWRHIPGARSGSVHLEDFPKVEGL
                     VDRGVLETWQRLLDVRQQVNAALEQKRKDKVIGTSLGARVELRASGPVAELLGRYRAD
                     LPMLLNVSEVSLVAAPSAGEDVVQVEVQKAAGEKCARCWRFVPTLRTEPEWAGICDRC
                     VDALGPVGR"
     gene            6897..7391
                     /gene="lspA"
                     /locus_tag="AC16CY_09245"
     CDS             6897..7391
                     /gene="lspA"
                     /locus_tag="AC16CY_09245"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3354014.1"
                     /translation="MSQARPTHISIVVLVALLDQVVKALVRPRLDMFESIPVITGFFS
                     LTRVHNTGMAYGLMNGVDFPFKTAVIALVQTTALVGFVFYAASLAPGQRLTRIGLAFV
                     IGGAVGNLIDRLTWGYVLDFFDFYWRGWHFWAFNIADASITIGVALMILDLLTGRSRV
                     PGTV"
     gene            7372..>7817
                     /locus_tag="AC16CY_09250"
     CDS             7372..>7817
                     /locus_tag="AC16CY_09250"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3354015.1"
                     /translation="MYPALFSIGPVTIYTYGVLLAASYLLGLRLAMVRARRWGLDANR
                     VLDLGIYIIIAALVGAKLMLLVVDFDQFRQSPWDLLALARSGGVFYGGLILALAVAFW
                     FIARHRMPFWTTCDVFAPGIALGHVTGRLGCFAAGCCYGKPTDVPWA"
BASE COUNT         1239 a         2589 c         2671 g         1318 t
ORIGIN      
        1 tgaacgacct tggacctcac gatgacgggc tcgtgggggc gatgcatcgg gcggcggcgg
       61 caatcgccaa agagcacggg cacgccgagg acggctaccg cacggtgttc aactgcaatc
      121 gcgccgcggg ccagaccgtg ttccacatcc acctgcacct gctggcaggg cgcggcctcg
      181 cgtggccgcc cgggtagcac gaaggtcacg aagacgatcc gccacgaagg acacgaagga
      241 caaggacgga acgcacatgg aacgaaggtc gaagcgtcgg cttctcctcg tggtcgttgc
      301 gctcggagcg gtcgtctcgg cagtggcgta ctggagcacg cgatcttcct gcatcgaggc
      361 ggggccgggc ttacgtggcg aagtgcaggt cctcgccggt aaaaccctgt acttcaacgg
      421 cgagtgctgg acgacggaac cgatgccgcc gcgcgacatg tctctctgac ccgcagcggg
      481 aggccgaccc gcccgatgtg tcctggagcg acggcgtgtc gcaagcccgg cgcgcgggca
      541 ggacacgtca ggcggggcga gccgctccga aacgggtcaa cccccgctcc ccaatagctt
      601 gggagttgga atttgggagt tgggatttac agaagggccg gcagttccgc catcgaccgg
      661 atgaccggca catccgccgc cggagccggg tcggaggagc gatggacgag cacgccgcgc
      721 atgcccacgc ggcgggcgcc gtcgatgtcg tgcgtgaggc tgtcgccgac catcaccgac
      781 tcccgcgcct ccacgccggc gagcgccagg gcggcctcga agatgctcgg gtgcggcttc
      841 atgtacccgt gttccgagga cgacacggct gccgtgatga ccccgcgcag ctcgaagtgc
      901 tcctgaaagg acgccaggca gcggtgcgag ttggagatca acccgatctt caggccgcgc
      961 cccgccagct cgcgcagcac cggggccacg tcgtcgtaga gctcgaagtg ctggcaggcc
     1021 gcccactcgc ggtagatctc gctggcgcat tcgtccagcc gcgggccggc accgcccatc
     1081 tcctcgatga tgcgcttggt gtagcagacg aagatgtccg cgtcgtacac gtggtcctgg
     1141 gcggcgtcga ggaccgacga tgccgcgcgc acggcctcgt cgaacttcgc ggcgtcgatc
     1201 gtcatcccgt agcgctcgca gaaccgcgcg tacccctcgc cctgaaacgt cgggccgggg
     1261 tagatcaagg tgaagtcaac gtcgaagaaa acggccgcgg cccggaaccg gctcacgcaa
     1321 gcgacccttc ataagggccc gcgacaacca cggtggcgcg cgcggggtcg atggcacgcc
     1381 gcgccgcctc gtgcacgtcc tggcggctca cggctcgaag cagtcctggc acgcgaacgt
     1441 cgtagtcgag cccaagcccg aagaactcag ccgattgcag gtagctcgca attcccacgt
     1501 tggtctccag cgtcctcggc agcgagccga tcaggtactg tttggactcg gtcgtctcct
     1561 gttcggtcgg accctcggcc gccagcctgg caagctcggc atcgatggac tggactgccc
     1621 gttcgacatt cgacggactt acgccggcgc ggatcaggag cggccctggc atgagactgg
     1681 cttcgagcga gctgaagacg taataggcca tcccctgctg ctcgcggatg cgatcgccca
     1741 ggcggccgcc cagcgagtac tgaccaagga tgttgttcat cagccagtac gcatagtacg
     1801 ccggatccga tcgcgcaatg gacacaaacc cgtacgcgat gtcggcctgc gacttgttca
     1861 tcatgggcac gacacgcgcg cgacgtgctc caggggccgg cgcgggttga cgtgtcacgg
     1921 gagcggccgc cccggccgtc cagccgtcga aggccgcacg cgcggcgtca atcgcccgct
     1981 ctgtctcgat gtcgccgacg aaggcgagcg acagggcgga cggtacgcag tgagcggcat
     2041 ggaattccag caacgcagcc cggtcgattc gctcgacgtt gtcgatcgtc ccgcgcaccc
     2101 ggcgcccgta gggatggcct cccggataca gcatctcgtt gagcgtttct acggccacga
     2161 ccgccggatt gtccgcgtcc tgccggatca gcgtgacgat ctccccccgc cggcgatcga
     2221 cttcgagggg gggaaaggtg ggccgcatca cgatctcggc gaccatcccg aggacgtcct
     2281 cgaagtcctc gacgaggcac gtgcacacca gggtgagcac gtgccggccc accgtcgtcg
     2341 cgagcgtcac accgcggccg tcgaagcgtt cggcaatctc gtcggccgag caccgctcgg
     2401 tgccccggtc gatcgcgcgg gacatgaagt gcgcgaggcc ttccccctcc gccgggtcgc
     2461 agtccgtgcc cgcgcgaatg ttggcgtgca gcgtcacggc aggggtcgtc cgggtctctt
     2521 tggcgatcac cgtcacgccg ttggccagca cggcacgcgc cggcgccagg cccgggcccg
     2581 acgtgtggga ctctgcgtga gcctgtatca aagaccggag tccttggctg gttcgaagcg
     2641 tccgatcgtc cggcccgccg ggttcaggcg ctcggcggcc acggcgtcta cctgatcggc
     2701 cgtcaccgac tcgagggcct ggccgagcga gagcagccac cgccacgacg cgatcgtctc
     2761 gaagaagccg agctggtggg cgatgttcgt gatgctgtcg ttttcgaaga cgagacgcgc
     2821 gcgcagctgg ttcttcgcct tggcgagctc ctggggcgtg acgccgcccg tgcgcacgcg
     2881 atcgagctcg gtgagggccg cctcctcgag acgatcgagc gtggtgccat cggtggccgt
     2941 ggcggagatg gtgtagagaa acggttcagc ggttggcgca agagccccgt tcaccgccga
     3001 ggcaagacca gaggcgacca gtgcctgata cagccgcgtg ctgcgctgcg gaggcggagt
     3061 ccggaagctc gccatgatgt tgaggccctt ggcgccggtg aggaccgcgt cgagcacgag
     3121 cagggcgaaa aagtcggggt cggttgccgc cggggcgtga acgccgatcc gcaggtacgc
     3181 ggtcgtgccc ttcttggcga tcgtcagacg gcgctcgccg agctgctgcg gctcgagcgt
     3241 gcgctgccgc cgcccgacgt cggccggctt catcgccccg aaatgcctca cgacggcgtc
     3301 catcacgttc gccgcctcca cgtcgccgac cacgacgagc gtcgcgttgt tcggcacgta
     3361 gaaccgcctg tagtgaccgt acagatcgtc gcgcgtcatc gtctgcagat cggacagcca
     3421 gccgatcgtc ggatgccggt agccgtgcgc gcggaacgcc gtggccgtca actcctggtc
     3481 gaggagctgc tccgggtcgt tctcgccccc ttgcagctcc gagatgatga cggtgcgctc
     3541 ggactcgcag tcggccgggt cgtagaggca gccctgcatc cgctcggcct caatgaacag
     3601 catgcggtcg agcgcgtccc gcgtggcggt ttctgtgtag gtggtctggt cgatccaggt
     3661 gtagccgttc cagtatccgc cgtacttctc gatgatgcct ttgacctgct cgcgcgggat
     3721 gttggtcgtg cccttgaagt tcatgtgctc gacccagtgg gacaccccgg tcagcccggg
     3781 cggctcgtcc ttcgagccga cgcggtacca gcaccacacg gtggccagcg gtgcggtgtg
     3841 gaccggctga atgagcacgc gcagtccgtt gtcgagaacc tcctcgcgca ccgccgaggc
     3901 gttctcggac gcgtgttcga gcgtggccat ccgcgaatta tagtaggcag taagccgtga
     3961 gcagctgagc gtgggcagac ggcggtcagt tagtgtgcaa acgctgctgc cagccgcacg
     4021 aggcggactg cccactgccc tgctgcgggc cgagcgccgc ctacgatcac acaacgacca
     4081 atgccagagt ggaaggacac agtcaatctg ccccgaacgg ctttccccat gaaagccagc
     4141 ttgcagaccg ccgagccgga agcgctggcc aggtggaagg cgatggatct gtacggtctc
     4201 attcgcgaga agcggcgcgg cgccccgaag ttcgtgttgc acgacggccc gccctatgcg
     4261 aacggccaga tccacctggg gaccgcactc aacaaggtcc tgaaggatct cgtcgtgaag
     4321 tcgcgcacca tggcgggctt cgacgcgcct tacgtgcctg ggtacgactg tcacgggctt
     4381 ccgatcgaac tgcgcgtcga gcgcgagctc ggcgcgaaga agcgcgacct gtcgatggcc
     4441 gacttccgca gggcgtgccg cgcgtacgcg gagcgcttca tcggcgtgat gacctccgag
     4501 ttccagcggc tgatggtgtt cggagactgg gaccatccct acgtgacgat gagcttccag
     4561 taccaggcgg atatcgcccg cgtgctcggc aagctcgtcg agcgggggct ggtctacaag
     4621 ggaaagaagc ccgtccactg gtgcatccat tgccggacgg cgcttgccga ggcggaggtt
     4681 cagtacgagg atcactcctc accgtcgatc ttcgtcgagt tcccgctgaa ccccgagagc
     4741 cggccggagc tcggctcgcg gttcccggcg ctcgccgggc gcgaggtgtc ggtcctcatc
     4801 tggacaacca cgccctggac gatcccctcg aacctggccg tcgcgttcca tcccgagttc
     4861 gactacgccg cctacgacgt ggacgggcgg gcggtgatcg tcgccgaggg tctcgccgaa
     4921 tcggtcggcc ggatcgtggg acggccgttc gaccgtcccg ttgcgcgcat gaagggcgcg
     4981 gcgctcgagg ggatcaggtt tcgccatccg ctctacgatc gggactctgt cggcgtcctg
     5041 gccgactacg tcacgctcga gcagggcacg ggcgcggtcc acacggcgcc cggtcacggc
     5101 gcggacgact tcctcaccgg catgaagtac ggcctcgaga tctatgcgcc cgtcgggccg
     5161 ggcgggcact tcctcgacac cgtccagctc tttggcggcc agcgcgtgtt cgacgcgaac
     5221 cccaacgtcg aacgggcgct cgaggcgcgg ggccggttgt ggcaccggga gatggtgtcg
     5281 caccagtacc cgcactgctg gcgctgtcac aatccggtga tcttcctcgc aacgtcgcag
     5341 tggttcgttc ggatggacgg tgaggcgtcg atcaacggcg gcgatggatc cctccgcacg
     5401 cttcgggacg cggcgcggca cgcgatcgat cacgaggtga gatggcttcc ttcgtggggc
     5461 cgcgatcgca tcttcaacat ggtgacgaac cggcccgact ggtgcatctc gcgacagcgg
     5521 gcgtggggcg tgccgatccc cgcagtagac tgcgtgaagt gcggcgaggc gatcctgacc
     5581 acccagctca tcgcgcgggc cgtgtcggtc ttcgagcagt acagcgccga cgcctggtac
     5641 gagcgcccga ttgaagagtt cctccctccc ggcctggttt gcccctcgtg cgggggcacg
     5701 tcgttcgaac gcgagcgcga catcctcgac gtgtggttcg attccgggtc gagccatgag
     5761 gcggtcctgc cgcgttaccc cgagctgacg tggccgtcgg acatgtacct ggaaggcagc
     5821 gatcagcacc gcggctggtt ccagagctcg ctcctcgtcg cgctggccac gcgcggccgg
     5881 ccggcctacc ggcaggtgct cacgcacggg tttctcatcg acctcgaagg ccagaagatg
     5941 tcgaagtcgc gggggaacgc gatggtgccg caggaggtga tcaaggacag cggcgcggag
     6001 atcatccgcc tctgggtcgc gatgacggag ttcaccgagg agctgcgcgt cagcaaggaa
     6061 atcctcacgc gcgtcgtgga cgtgtaccgg aagctccgga acacctgccg gattctcatc
     6121 ggcaacctct acgacttcga tcccgcgacc gacctggtcc cgctggcaca gctggacgaa
     6181 gtggaccggt acgcgctggc gcgctacggc gaggccgctg aacggatgct ccgcgcgtac
     6241 gacgagtacg acttttcgac cgtgtcgcag gcgctgaata cgctggcgac ggtggatctc
     6301 agcgcgttct acgtggatgt caccaaggac cggatgtaca cgctcggcgc ccggtcccgt
     6361 gagcgccgtt cgacgcagac cgcgatgtac ctgatctccg aggggctggc gcggctcatg
     6421 gcgccgattc ttccggcgac agccgacgat ctctggcggc acatccctgg tgcccggtcg
     6481 ggctccgttc atctcgagga ctttcccaag gtggagggcc tggtggaccg gggagtgctc
     6541 gagacgtggc agcgcctgct ggatgtgcgc cagcaggtca atgccgcgct cgaacagaag
     6601 cggaaagaca aggtgatcgg cacctcgctg ggggcccggg tcgagctgcg ggccagcggt
     6661 cccgtggccg agctgctcgg ccgctaccgc gccgacctgc ccatgctgct caacgtgtcg
     6721 gaggtgtcgc tcgtggcggc gccttccgcg ggcgaggatg tcgtccaggt ggaggtgcag
     6781 aaggccgccg gtgaaaaatg cgccaggtgc tggcgcttcg tccccacgct gcgcaccgag
     6841 ccggagtggg cgggcatctg cgaccggtgc gtggatgcgc tggggccggt cgggcgatga
     6901 gccaggcccg cccgacgcac atctcgatcg tcgtgctggt cgcgctgctg gaccaggtcg
     6961 tgaaagcgct cgtccgtcca aggctcgaca tgttcgagtc gattccagtc atcacggggt
     7021 tcttcagcct cacccgcgtc cacaacaccg gcatggcata cgggctgatg aacggggtcg
     7081 acttcccttt caagacagcg gtcatcgcgc tcgtgcagac aacggcgctt gtcggtttcg
     7141 tcttctacgc ggcgtcgctg gcccccggcc aacggctgac gcgcatcgga ctggcgttcg
     7201 tgatcggcgg cgccgtcggt aacctgatcg accggctgac ctggggctac gtgctcgatt
     7261 tcttcgactt ctactggaga ggctggcact tctgggcctt caatatcgcc gacgcgtcca
     7321 ttacgatcgg agtcgcgctg atgattctgg acctcctgac aggacgttcc cgtgtacccg
     7381 gcactgttta gcatcggccc cgtcacgatt tacacctacg gcgtgctgct ggccgcgtcg
     7441 tacctcctgg ggctgcgcct cgcgatggtc cgggcccggc ggtggggact cgacgccaac
     7501 cgcgtcctcg acctcggcat ctacatcatc atcgcggcgc tcgtcggcgc gaagctcatg
     7561 ctgctcgtcg tcgacttcga tcagttccgc cagtcgccgt gggatctgct cgcgctggcg
     7621 cggtcgggcg gcgtgttcta cggcggcctc atcctggcgc tcgccgtggc gttctggttc
     7681 atcgccaggc accggatgcc gttctggacg acgtgcgacg tctttgcgcc gggaatcgca
     7741 ctgggtcacg tcacgggacg gctcggctgc ttcgcggccg gatgctgcta cggcaagccc
     7801 accgacgtcc cgtgggc
//
LOCUS       DBNGSH010000403        12444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2074132, whole genome shotgun sequence.
ACCESSION   DBNGSH010000403 DBNGSH010000000
VERSION     DBNGSH010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2074132"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC16CY_09255"
     CDS             <1..482
                     /locus_tag="AC16CY_09255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3354016.1"
                     /translation="DVRRVLAYLESVVGPAASDMPRSLTWQMIAEMGGAGFTIGSHTK
                     THASLPMETPEAAVEEIAGSKRELEAHLGRPVVHFAYPGGQFTPGVVDAVAAAGYQCG
                     YTACPHRDPRYPMVTMERLLLSEGSSIDATGRFSPPILSCQVHDLWPPARRCEWNHA"
     gene            479..1945
                     /locus_tag="AC16CY_09260"
     CDS             479..1945
                     /locus_tag="AC16CY_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3354017.1"
                     /translation="MRDLLRLDGVSRPALWLIVGRGVGLLAAVVVPLVIVRLFDQAAF
                     GTYKQLFLIYGTLFGVAQLGAAETLYYFVPRSPAEAGRLASNAVVTLAVVGVACVALL
                     GLTAAPIATWLMNPELAGTLLLLGVMLALMLLAAPFEIVLVSNQQYKAAAYTYALSEF
                     VRAGFILVPAMVFGGLRAVLVGAIVFAAVRVLAMLWSFWRHLGATFRPSLSLWRQQCV
                     YTLPFALAVGMEAVQGNVHHYVVASRFDPATFAIYAVGCLQIPLVDVITSSSANVMMV
                     KMSKDGFDSRGPAALALWHDTTRRLALVIFPLAAFLLVMARDIIVVLFTSNYLASVPI
                     FMLWTLSIVFSVPCVDALLRVHAQTRFLFGLNVLRLALVLGLTAWFLSLYGLPGAVLV
                     TLLSTAIVRVAGMARIARLLGIGLGAVMPWTRLAMTAAYAAIAAVPTFWFSHTSSLPR
                     VLVLISAAAIYGATYAALCYGLSRTPVRVAPALQDVAP"
     gene            1980..3887
                     /gene="asnB"
                     /locus_tag="AC16CY_09265"
     CDS             1980..3887
                     /gene="asnB"
                     /locus_tag="AC16CY_09265"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3354018.1"
                     /translation="MCGIAGIIRWDGRTVFERELRTMCRAMIHRGPDEEGVYLGDGVG
                     LGMRRLSIIDVDTGQQPVSNEDRTVWVVFNGEIYNYRELRRDLESRGHVFRTSSDTET
                     IVHLYEELGPRCVNLLRGMFALAIWDESRRQVLLARDRLGIKPLYYAERDGELVFASE
                     LKPILQLAHIDRSLNWEAVGHLFTFLATSPAQSIADGVKKLEPARLAIASRSRPLRTE
                     RYWDVTFEPNERATEPELVERLRELLAESVALHQVSDVPVGAFLSGGIDSSAVVATMS
                     RMNAQRVKTFSVGFREAEFDELRHARRVAALNGTDHYDLVLEPEVVPMVEDLAWYLDE
                     PFGDTSAIPTYMVSKLAAEHVKVVLTGDGGDEIFGGYDKYLKEGRERTYDRVPRPIRK
                     VASAVGDAMPEGMRGRRFLRHLALDGSERYLHAQALFHADEMRRLFHPDAFAEMAHHD
                     PMAASRSHLTGGRDWISAVQYGDLQTYLPLDVLTKVDRMTMAHSVEARPPLLDHRLVE
                     FAATIPARFRLRGGTTKYLLKQAMRGILPDDIIDRRKQGFAVPLAKWFRGPLAGFARD
                     VLLSDSCRRRGFLNTDYIEQLLRLHQRGRNLDLQLWTTLSFEMWCRRFLDRGIAQPRV
                     SHSPSPTLHVA"
     gene            3891..5012
                     /locus_tag="AC16CY_09270"
     CDS             3891..5012
                     /locus_tag="AC16CY_09270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3354019.1"
                     /translation="MTRPHVAIVAASLEILGGQGVQAHGLVGGLRQDGHSVRFIPINP
                     RFPTGLQWARRLRYLRTIVNQALYLPSLARLAGSDVVHVFSASYLSFLLAPVPAMLIA
                     RVLHKRVVLHYHSGEAGDHLAHWGLLVHPWLRLADVIVVPSEYLADVFQAHGYQTRVV
                     RNVVDLSRFEYRERQDRRPRLLSTRNLEPYYRIDVILEAFALVKAQRPDATLTVAGYG
                     SEENRLRRLAGEGVRFAGKVDPQSMPRLCDEADIFLNASVVDNQPVSILEAFAAGLAV
                     VSTPAGGIPEMVRHTHTGLIVPVGDAASLAAAVLHLITHPREASAMARQARQDVTRYT
                     WLAVRDQWSAVYDGSRDPALFDHPIRPRETEPWTPHSTQ"
     gene            5108..7249
                     /locus_tag="AC16CY_09275"
     CDS             5108..7249
                     /locus_tag="AC16CY_09275"
                     /inference="COORDINATES: protein motif:HMM:NF028199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HIR3354020.1"
                     /translation="MPVSEIAYRGRQEASKWLDRVSATARPVDPETWLKAHAPTLATP
                     DASLRLLREHLPRRFFASVAEPCTASALHTRFPDARREVVIAADTLMDGRFDLLGYRG
                     LWFGDPIDWHLDPVWSRRSPLAHWSRLDPLDPSMVGDSKIVWELNRHQWLVRLAQAWT
                     VTGDERYAAACIEAIDAWRDANPPGVGINWASSLEVAYRLMSWCWTALLIRDAPMLSG
                     SWMMRLLVAIGQHANHITRYLSYYSSPNTHLTGEALGLFYAGTLFPEYRDAARWGRVG
                     TRVLLAESRAQLLSDGAHFEQSTCYQAYTVDTYLQYLLLANRNGVTLPRELLEQVAHM
                     VAFHLAVRQPDGSIPAIGDSDGGRLLPLVARQRGDSRGLFAVAAAVFDRPDFAWAADG
                     AAPEILWLMGSSGLQAFDAVSPAPPSDGASRVFPSGGYGVMRSGWEPDAHQLIVDVGP
                     LGCPTTSGHGHADLLSVQCAIFGEACLVDAGTYCYTTERPWREFFRSTAAHSALRVDG
                     LDQTQSVGPFRWDRRPRARLRSWQSDPDRDVLDADHDAYLRLPDPVACRRRVIFVKPD
                     YWLVVDDVDGASSHQIEVAFQFAPTMQVTVGPQPWVRANTPGGRVLWMLSLSSSPVQT
                     TLNFGARDPIRGWTSSEYGQRQPAPMLVFSSSVALPWRGLTLLLPDSEGLAVPPVVTP
                     VWDREGRPTGLTFDRPRRSVRVDDSAVVITA"
     gene            7293..9269
                     /gene="asnB"
                     /locus_tag="AC16CY_09280"
     CDS             7293..9269
                     /gene="asnB"
                     /locus_tag="AC16CY_09280"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3354021.1"
                     /translation="MCGIAGIVSLDSGALIDDARLKRMRDALIHRGPDGTGLHVAGRV
                     GLAHTRLAIIDVAGGEQPMRNETGDVWIVFNGEIYNHADLRPRLEDRGHRYQSRCDTE
                     TILHLYEETGDRVVEHLRGMFAFAIWDHTRQRLLLARDRLGIKPLYYAVTDRELLFAS
                     EIKALLAAGSIRPAFNEGVLPEFLSTRFVSGDETFFRGVQKLLPGHVLTWSARERLQT
                     RRYWQIPAPAGETSSGATATPEDLRERLEAAVSSHLMSDVPLGVFLSGGVDSSALAAL
                     VARATSGPLQTFAVGFADREANELPYARLAAAHVGAEHHEVTVTPSEFFQALPHALWH
                     EDEPIAFPSSVPLYFLARLARNHVKVVLTGEGADELFLGYNRYRVTKWNARLGQPYWA
                     AVPPAVRRHVRRAVRALPTPISRLARRTFIGLEPGVRDLYLENFAVFPVALQQSLLRR
                     SDLLEQRDPYAAELQYYGEATGGLLDRISRADLQTYLLELLMKQDQMSMAASIESRVP
                     FLDDRLVEHVAAIPGDVKLPRWHTKALLRDAVRDLIPPEILTRPKMGFPVPLGRWFRE
                     GFAPIVDEFVLGPRAEARGYFHRSALQRMATAHRLGQGAHADALWLLVNLEIWLRVFC
                     DGEAPGDVMQAVRFTDAQAARQSSVQRVQCASSG"
     gene            9251..10513
                     /locus_tag="AC16CY_09285"
     CDS             9251..10513
                     /locus_tag="AC16CY_09285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3354022.1"
                     /translation="MRILWVKMGGLWPPTSGGRVRSLEMLSCLTRRHEVTVLTTHGPD
                     DDTSGLAARLPGCRRVISVPFAAPRLGSASFARALARSWLSSLPVDLWKWRVGAIRDE
                     ARALIADGAVDLCVADFLVAVPNIPRSNRVPLVLFEHNVEYMIWRRIAALESRPWRRA
                     LLEIEWRKVRRIERLACSRADITITVSEEDQRLLQELAPDGRCVAVPTGVDAAYFAPG
                     GRPEVPHRLVFTGSMDWHPNEDAILHFSRSILPRIRAQVPDVTITVAGRTPSAHLREV
                     AGQAGIVLTGTVDDVRPYIDEAAVYIVPLRAGGGTRLKIFEALAMAKAVVSTTVGAEG
                     LALTPDRDVCIADEPEAFAATVIALLRDPARRHALGRAGRQLVEDRYSWDRVAGHFDE
                     YCQSAIARADTRTGAGALDAMLGRSSSL"
     gene            10620..11942
                     /locus_tag="AC16CY_09290"
     CDS             10620..11942
                     /locus_tag="AC16CY_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR3354023.1"
                     /translation="MRVAVLGLGYVGCVTAACLARDGHEVIGVDVNADKVAMITAGQS
                     PIIEPGLEELLARVVQDGRLSATAQTENALASSDVALICVGTPDIGHGQQDLTALSHV
                     AAAIGTACLGRSRPLTVILRSTVLPGTTARVLQRILIDTAQHYSSTVHIAVNPEFMRE
                     GSSLEDFQNPPFILAGCDDDETASVLRRLYAGVDAPFLQTTVRTAEMVKYVCNAYHAL
                     KICFANEVGDLCDALYTDGQEVMRIVCEDRKLNISAAYLTPGFAFGGSCLPKDVRALL
                     SAGRIADIPLPLLGAIVSSNDGLIRRGVDAVLRTRRRRVGVVGLAFKPTTDDLRESPM
                     VALVEALIGKGCDVRIYDPTVVLASLKGANRRYIEAEIPHISSLLCDSVDALITHSDV
                     LVFGATGVQAERVMAAATDRQAIVDLTHGALVRHVERASDLVGLEVEP"
     gene            11939..>12444
                     /locus_tag="AC16CY_09295"
     CDS             11939..>12444
                     /locus_tag="AC16CY_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354024.1"
                     /translation="MTARTFVACGVAPVILLAMVAATRPEAVRPAGSPGPADSRGALQ
                     GALPEGPRESVDADTIPEPKRRIQVSASGDLQRALDQAQPGDQIELENGATYQGPFRL
                     RDKQGDGWIVVTSADPRGLPAPGERVSPSHAPRMAKLRASHGAVIAADAGAHHYRFIG
                     LEIAPSAGT"
BASE COUNT         1862 a         4227 c         4122 g         2233 t
ORIGIN      
        1 gggatgtcag gcgcgtgctg gcgtacctcg agtcggttgt cgggccggcc gcgagcgaca
       61 tgccgcgctc gctcacatgg cagatgattg ccgaaatggg tggggccggc ttcaccatcg
      121 gctcgcacac gaagacgcat gcgtcgttgc ccatggaaac gccggaggct gctgtcgaag
      181 agatcgcggg atcgaagcgc gagttggagg cgcacctcgg aagacccgtc gttcatttcg
      241 cctatccagg cgggcagttc accccggggg tcgtggacgc cgtggcggcc gccgggtacc
      301 agtgtggcta caccgcatgt ccgcatcgcg acccacggta tcccatggtc acgatggaac
      361 gcctgctgct ctcggagggg tcctcgatcg atgcgaccgg caggttctct ccccccattc
      421 tgagctgcca ggttcatgat ctctggccgc ccgcccgccg gtgcgaatgg aatcacgcat
      481 gagagacctg ctccggctgg acggcgtgtc gcggcccgcg ctgtggctca tcgtcggccg
      541 cggggtgggg ttgctggcgg ccgtcgtcgt ccccctcgtg atcgtccggc tcttcgacca
      601 ggccgcgttt gggacgtaca aacagctgtt cctcatctac ggcacgctct tcggggtcgc
      661 tcaactcgga gcggccgaga cgctctacta cttcgtgccg cggagtcccg ccgaggcagg
      721 ccggcttgcg tcgaacgccg tggtcacgtt ggccgtcgtc ggcgtcgcct gcgtggcgct
      781 gctcgggctg acggcagcgc ctatcgccac gtggctgatg aatccggaac tcgcgggcac
      841 actcctcctt ctcggcgtga tgctcgcgct gatgctgctg gcggctccat tcgagatcgt
      901 cctggtgtcg aaccagcagt acaaggcggc ggcgtacacc tacgctctct cggagttcgt
      961 gcgcgcggga ttcatcctcg taccggcgat ggtgttcggt ggtctgcgcg ccgtgctcgt
     1021 cggcgcgatc gttttcgccg ccgttcgtgt cctcgccatg ctgtggtcgt tctggcgcca
     1081 cctcggggct acgttccggc ccagcctgtc gttgtggcgc cagcagtgtg tctatacgct
     1141 cccctttgct ctggccgtcg gcatggaagc ggtccaaggg aacgttcatc actacgtggt
     1201 ggcctcccga ttcgacccgg cgacgttcgc gatctacgcc gtggggtgcc tccagatccc
     1261 cctcgttgat gtgattacgt cctcgagcgc caacgtcatg atggtcaaga tgtccaagga
     1321 cgggttcgac agccgaggtc ccgcggcgct cgccctctgg catgacacga cgcgccggct
     1381 cgccctcgtg atctttccgc tcgcggcgtt cctcctcgtg atggcccgag acatcatcgt
     1441 cgtgctgttc acgagcaact accttgcgag cgtgccgatc ttcatgctgt ggacgttgag
     1501 catcgtcttc tccgtcccct gcgtcgacgc gttgctccgg gtccatgccc agacgcgctt
     1561 cctgttcggc ctgaacgtgc tgcggctggc gctcgtgctc ggcctcaccg cgtggttcct
     1621 gtcactgtat ggactgccgg gtgcggtact ggtgacgctc ctgtcgacgg ccatcgtccg
     1681 cgtcgcgggc atggcccgca tcgcccggct cctcgggatc ggccttggcg ccgtgatgcc
     1741 gtggacgcgt cttgcgatga ccgcggcata cgctgcgatc gccgccgttc cgacgttctg
     1801 gttctcgcac acctcatcat tgccgcgtgt gctggtcctg atcagtgcgg ctgcgatcta
     1861 cggggcgacc tacgccgccc tctgctacgg cctcagtaga acaccagtgc gggtcgcgcc
     1921 ggccctgcag gacgtggcgc cgtaacgcgc gccaggtctg actctcaaag gacatcacca
     1981 tgtgcggcat cgctggaatc atcaggtggg acggccggac ggtgttcgag cgcgagctgc
     2041 gaaccatgtg cagggcgatg atccatcggg gacctgacga ggagggggtg tatctcggcg
     2101 acggcgtggg gctcggcatg cggcggctga gcatcatcga cgtggacacc ggccagcagc
     2161 ccgtgtcgaa cgaggatcgc accgtctggg tcgtcttcaa cggcgagatc tacaactacc
     2221 gcgagctgcg gcgcgacctc gagagccggg gccacgtgtt ccggacgtcc agcgacacgg
     2281 aaacgatcgt gcatctctat gaagagctcg gacctcggtg cgtgaatctg ctgcgcggaa
     2341 tgttcgcgct ggccatctgg gacgagtcac gacggcaggt gctgttggcg cgcgatcgat
     2401 tgggcatcaa gccgctctac tacgcggaac gtgacggcga gctggtgttc gcctcggagc
     2461 tcaagccgat tctgcaactg gcccacatcg accgctcgct gaactgggag gcggtcgggc
     2521 acctgttcac cttcctcgcc acctcgccgg cgcagagcat cgctgacggc gtgaagaagc
     2581 tggagccggc acgactcgcg atcgcgagcc ggagccgccc gctgcggacc gaacggtact
     2641 gggacgtcac gttcgagccg aacgagcggg caaccgagcc cgagctggtc gagcggctgc
     2701 gggagctgct ggccgagtcg gtcgcgctgc atcaggtgag cgacgtgcct gtcggcgcct
     2761 tcctgagcgg aggcatcgac tcgagcgcgg tggtcgcgac catgtcgcgg atgaacgccc
     2821 agcgggtgaa gactttctcg gtcggattcc gcgaggcgga attcgacgaa ctgcgccacg
     2881 ccagacgggt cgcggcgctc aacggcaccg accactacga cctcgtgctc gagcccgagg
     2941 tggtgcccat ggtcgaggac ctcgcgtggt acctcgacga acccttcggc gatacgtctg
     3001 ccattcccac ctacatggtg tcgaagctgg ccgccgagca cgtgaaggtc gtgctcaccg
     3061 gcgacggggg agacgagatc ttcggcggct acgacaagta cctcaaggaa ggccgggaac
     3121 gaacctacga ccgcgtgccg cggccgatcc gaaaggtcgc cagcgcggtc ggtgacgcca
     3181 tgccagaggg gatgcgcggc cgccggttcc tccgtcatct ggcccttgac ggatccgaga
     3241 ggtatctcca cgcccaggcg ttgttccacg ccgacgagat gcgcaggctg ttccaccctg
     3301 atgcgttcgc ggagatggcc catcacgacc ccatggcggc atcccggtca catctgacgg
     3361 gcgggcgtga ctggatatcc gccgtgcagt acggcgacct ccaaacctac ctgccgctcg
     3421 acgtcctgac caaggtcgat cggatgacca tggcgcactc ggtcgaagca cgaccgccgc
     3481 tgctcgatca ccggctcgtg gagtttgcgg cgacgatccc ggcccgcttc cggcttcgtg
     3541 gaggaaccac gaaatacctg ctgaagcagg ccatgcgtgg catcctcccg gacgacatca
     3601 tcgaccggcg caaacaggga ttcgccgtgc cgctggccaa gtggttccgt gggcccctgg
     3661 ccggcttcgc ccgcgatgtg ctgctgtccg acagttgccg gcgccgtggc ttcctgaaca
     3721 ccgattacat cgagcagctg ttgcggctcc accagcgcgg ccggaacctc gatctgcagt
     3781 tgtggacgac gctgtccttc gaaatgtggt gccggcgttt cctcgaccgg ggaatcgcgc
     3841 agcctcgcgt gagtcactcg ccgtcgccaa cgctccacgt ggcctgattc atgacacgac
     3901 cccatgtcgc cattgtcgcc gccagcctcg agatcctcgg cggtcagggc gtgcaggccc
     3961 acggcctggt gggcggcctc cgccaggatg ggcattccgt ccgcttcata ccgatcaatc
     4021 ccagatttcc cacgggcctt cagtgggcac gccgactccg ctacctccgc acgatcgtca
     4081 accaggcgct gtacctcccg agcctcgcca gactcgccgg ctcggatgtc gtacacgtct
     4141 tctccgcgtc gtacctgtcc ttcctgctcg cacccgtgcc ggccatgctg atcgcgcgag
     4201 tcctgcacaa gcgcgtcgtg ctccactacc acagcggcga agccggcgat cacctcgccc
     4261 actggggcct gctcgtccat ccctggttga gactcgccga cgtgatcgtc gtcccatcgg
     4321 agtacctggc tgacgtattc caggcgcacg ggtatcagac gcgggtcgtt cgcaacgtcg
     4381 tggatctgtc acggttcgag tatcgcgagc gtcaggatcg tcgtccacgc ttgctctcga
     4441 cacggaacct cgaaccgtac taccgaatcg acgtgattct cgaggccttc gcgctcgtga
     4501 aggcacagag accagacgcg acgctgaccg tggcggggta cgggagcgag gagaatcggt
     4561 tgcgtcgtct ggccggggag ggggtccggt tcgccgggaa ggtggacccg cagtcgatgc
     4621 cgcgactctg cgacgaggcc gacatcttcc tcaacgcgtc ggtcgtggac aatcaaccgg
     4681 tctccattct cgaggcgttc gcggctggcc tcgcggtggt gtccacgccg gccggcggta
     4741 ttccagagat ggttcgtcac acccacaccg ggctcatcgt cccggtcggc gacgcggcgt
     4801 ccctggcggc ggccgtcctt catttgatca ctcatccgcg cgaagcgtcc gcgatggcgc
     4861 gacaggctcg gcaggatgtg acccgatata cgtggctcgc ggtcagagat cagtggtcgg
     4921 cggtgtatga cggatctcgg gaccccgcgc tcttcgatca ccccattcga ccgcgtgaga
     4981 ccgaaccatg gacgccccat tcaacccagt gacgcccgaa tcagcgagcg gggcccgtgc
     5041 cggaccggcg tggggctcca cacgtggcgc ccgactgagt gcgcggcctc tccgcaggct
     5101 ccaggcgatg cctgtgtcgg agatcgccta ccgcggccga caggaagcct cgaagtggct
     5161 cgaccgtgtg tcggcgaccg cacgtcccgt cgatccggag acgtggctca aggcgcatgc
     5221 cccgacgctg gctaccccgg acgcatcgct ccggcttctc cgggagcacc tgccgcgccg
     5281 cttcttcgcg agcgtggccg aaccgtgcac cgcgtcggcg ctccacactc gattccccga
     5341 tgcgcgtcgc gaggtcgtga tcgcggccga caccctcatg gacgggcgat tcgatctgct
     5401 tggttaccgg gggttgtggt tcggcgatcc gatcgactgg catctcgatc ccgtctggtc
     5461 gaggcgaagt ccgctcgcgc attggagccg tctcgatccg ctcgatccga gcatggtcgg
     5521 cgacagcaag atcgtctggg agctcaaccg gcatcaatgg ctggtgcgat tggctcaagc
     5581 gtggacggtc acgggtgacg agcgctatgc ggcagcgtgc atcgaagcga tcgacgcctg
     5641 gcgagacgcg aatcccccag gcgtcggcat caattgggcc agcagcctcg aagtggccta
     5701 ccgcctgatg tcgtggtgct ggacggccct cctcattcgg gacgcaccca tgctctcggg
     5761 ctcgtggatg atgcggctcc tcgtggcaat cggccagcac gcgaatcaca tcacgcggta
     5821 cctgtcctat tactcctcgc ccaacaccca tctgacggga gaggcactgg ggcttttcta
     5881 tgccgggacg ctctttcccg agtatcgtga cgccgcgcgg tggggccgcg tcggcacgcg
     5941 ggtgctcctc gccgagagcc gggcccaact gctgtccgac ggcgcccatt tcgagcagtc
     6001 cacgtgctac caggcctaca ccgttgacac gtacctgcag tacctccttc tggccaatcg
     6061 caatggcgtg acgcttcctc gagagctcct cgagcaggtg gcacacatgg tcgcgttcca
     6121 tctcgcggtt cgccaaccgg atggatcgat tcccgcgatt ggcgatagcg acggcggcag
     6181 gctgcttcct ctggtggcgc gtcaacgggg agacagccgg ggtctgttcg ctgtggcggc
     6241 ggcggtcttc gaccgaccgg attttgcgtg ggcggctgac ggcgcggcgc ccgaaatcct
     6301 ctggctgatg ggcagtagcg gcctgcaggc cttcgatgcg gtgagtccgg cgcctccctc
     6361 tgatggggca tcccgggtgt tcccctctgg tggatacggg gtcatgcgct ccggatggga
     6421 gcctgatgcc caccaactga tcgtcgatgt cggtcccctg ggctgtccga cgaccagcgg
     6481 acatggccat gccgacctac tgagcgtcca gtgcgcgatc ttcggagaag cctgcctcgt
     6541 ggatgcgggc acgtactgct atacaacgga acgcccgtgg cgggagtttt tcaggagcac
     6601 cgcggctcat agcgccctgc gagtggacgg gctggaccag acgcagtcgg tgggaccgtt
     6661 ccggtgggat cggcgtcccc gcgcgcggtt gcgctcgtgg cagtccgatc cggaccgtga
     6721 cgtcttggac gcggatcatg atgcctatct gcgcctccct gacccagtgg cctgtcggcg
     6781 gcgcgtgatc ttcgtgaaac cggactactg gctcgttgtt gacgacgttg atggcgcgtc
     6841 gagtcatcag atagaggtgg cattccagtt cgcgcccacg atgcaggtca ccgtgggacc
     6901 acagccgtgg gtccgcgcga acacgcccgg agggcgagtg ctgtggatgc tgtccctgtc
     6961 atcgagcccg gtgcagacca cgctcaattt cggtgcgcgc gatcccattc gtgggtggac
     7021 ctcgtccgag tacggacaac gccagccggc cccgatgctc gtcttctcct cgagcgtggc
     7081 gctgccgtgg cgcgggctga cgctactcct ccccgactcg gagggattgg cagtgccgcc
     7141 ggtcgtcaca cccgtctggg atcgcgaggg tcgccccacc gggctcacct tcgatcgtcc
     7201 gcgtcgttca gtacgcgtgg acgattccgc cgtggtcatc acggcctaga ccactccgtt
     7261 ctcgtccact tcacgccaag gatctgtcct ccatgtgtgg catcgcaggc atcgtcagcc
     7321 ttgattccgg cgcgctcatc gacgacgccc gcttgaagcg gatgcgcgat gcgctgatcc
     7381 atcggggtcc ggacgggaca ggcctccacg tcgccggacg tgtggggctg gcgcacacgc
     7441 gtctggcgat catcgacgtg gccggcggcg aacagccgat gcgcaacgag accggcgacg
     7501 tctggatcgt cttcaacggt gagatctaca accacgccga cctccggccc cgcctggagg
     7561 atcgcgggca ccgctaccag tcgcgatgcg acaccgagac catccttcat ctctacgagg
     7621 agacgggcga tcgcgtcgtc gagcatctcc gcggcatgtt cgcgttcgcg atctgggacc
     7681 acacacggca gcgactcctg ctggcgcgcg accggctggg catcaagccc ctctactacg
     7741 cggtcaccga ccgcgaactg ctgtttgcgt cggaaatcaa ggcgctgctg gcggcgggat
     7801 ccattcgacc ggcgttcaac gaaggcgtcc tgcccgagtt cctctccacc cggttcgtgt
     7861 cgggtgacga gacattcttc cgaggcgttc agaagctgct ccctggacac gtcctcacat
     7921 ggtcggcacg cgagcggctc cagacgcggc ggtactggca gattcccgcc cctgccggcg
     7981 agacctccag tggcgccacc gcgacgccag aagaccttcg cgaacgcctg gaggcagccg
     8041 tctccagtca cctgatgagc gacgtgcctc tcggcgtgtt cctctcgggc ggcgttgatt
     8101 cgagcgcgct ggccgcgctc gttgcgcgcg ccacgagcgg gcccctccag acatttgccg
     8161 tcggcttcgc ggatcgtgag gccaacgagc tgccgtacgc gcggctcgcg gcggctcacg
     8221 tcggcgctga gcaccacgag gtcacggtga cgccgtcgga attcttccag gccctgccgc
     8281 acgcgctctg gcacgaggac gagccgatcg cgtttccgtc gagcgtgccc ctctattttc
     8341 tggctcgact ggcgcgcaac cacgtcaagg tcgtgctgac cggcgagggc gccgacgagc
     8401 tgtttctggg ctacaaccgc taccgggtga cgaagtggaa cgcgcgcctg ggacagccgt
     8461 actgggcggc agtgccaccc gccgtgcggc gccacgtgcg tcgcgccgtg cgcgcgctgc
     8521 ctacgcccat cagccgcctg gcgcggcgca cgttcatcgg gctcgagccc ggtgtccgcg
     8581 acttgtacct cgagaacttc gccgtcttcc cggtcgcgct gcagcagtcc ctgctccggc
     8641 ggtcagacct cctcgagcag cgggatccgt acgccgctga actgcagtac tacggggagg
     8701 ccacgggagg gctgctcgac cgcatcagcc gggcggacct ccagacatac ctgctcgagc
     8761 tgctgatgaa gcaggaccag atgagcatgg cggcgtcgat cgaaagccgc gtccccttcc
     8821 tcgacgatcg tctggtcgaa catgtcgcgg cgattccggg cgacgtcaag ctgccccggt
     8881 ggcacaccaa ggccctgctc agggacgcgg tgcgggacct gatccctccg gagatcctca
     8941 cacgcccgaa gatgggattt cccgtgccgc tcggccggtg gttccgggaa gggttcgccc
     9001 ccatcgtcga cgaattcgtc ctgggaccgc gggccgaggc ccgggggtac ttccatcgct
     9061 ctgctctgca gcggatggcc acggcccacc gcctgggaca gggcgcacac gccgacgcgc
     9121 tgtggctgct cgtcaatctc gagatctggc tgcgcgtctt ctgtgacggc gaggcgcctg
     9181 gcgacgtcat gcaggcggtt cggttcactg acgcccaggc ggcccgccag tcctcggtgc
     9241 aaagggttca atgcgcatcc tctgggtgaa gatgggcggc ctgtggccgc caacgtcggg
     9301 aggccgcgtt cgcagcctgg agatgctgtc ctgcctgacg cggcggcatg aggtgaccgt
     9361 gctgacgact catggccccg acgacgacac gagcggtctc gcggcgcggt tgccgggctg
     9421 ccgtcgagtc atatcagtcc cgttcgcggc cccacggctc ggctcggcct ccttcgcgcg
     9481 tgcgctggcc cggtcctggc tgtcgtcctt gccggtcgat ctctggaagt ggcgagtggg
     9541 cgcgattcgc gacgaggcgc gcgctctcat cgccgacggc gcagtcgatc tctgcgtcgc
     9601 ggacttcctg gtggcggttc cgaacatccc acgatcgaat cgcgtcccgc tggtgctctt
     9661 cgagcacaac gtcgaataca tgatctggcg ccgcatcgcc gcgctcgagt cgcgcccgtg
     9721 gcgccgggcc ctgctcgaga tcgaatggcg gaaggtgcgg cggatcgaac gtctggcctg
     9781 ttcgagggcc gacatcacga tcaccgtgtc cgaagaggat cagcggctgc tgcaggagct
     9841 cgcaccggac ggccggtgcg tggccgtccc gaccggtgtc gatgccgcgt atttcgcgcc
     9901 cggcgggcgg ccggaggtgc cgcaccggct cgtgttcacc ggctcgatgg actggcatcc
     9961 gaatgaggac gccatccttc atttcagccg gtcgatcctt ccgcgcatcc gcgcacaagt
    10021 gcctgacgtc acgatcaccg tggccggacg gacgccgagc gcgcatctgc gcgaggtcgc
    10081 cgggcaggcg ggcatcgtgc tgaccggcac cgtcgatgac gtgcgtccct acatcgacga
    10141 agccgccgtc tacatcgtcc cgctgcgcgc gggcggcggg acccggctga agatcttcga
    10201 agccctcgcg atggccaagg ccgtcgtctc gacgacggtg ggcgccgagg gactggcgct
    10261 cacgcctgac cgcgacgtct gcatcgcgga tgagcccgag gcgtttgccg cgaccgtgat
    10321 cgcactcctc cgggaccctg cccggcgtca cgccctcggc cgggccggcc gccagctcgt
    10381 cgaggaccgg tattcctggg atcgggttgc cggtcatttt gacgagtact gccagtccgc
    10441 gatcgcccgg gcagacacac gcactggtgc gggtgccctc gacgcgatgc tcggacgctc
    10501 gagttcgctc tagaaatcac ctcaaccgcc gtcgcggact cgcgacgcgc ccccgcgttc
    10561 gccgaggcca tcccgatggc ccgctgcatg ccctaccctc ggctcagagg aggctcgcca
    10621 tgagagtcgc agtgctcggt ctcggctacg ttggatgcgt cacggcagcc tgtctcgcgc
    10681 gcgacggcca cgaggtcatc ggcgtcgatg tcaacgccga caaggtcgcc atgatcacgg
    10741 ctggccagtc gccgatcatc gagccggggc tcgaggagct gcttgcccgc gtcgtgcagg
    10801 acgggcgcct ctcggccacc gcgcagaccg agaacgccct cgcgtcgagc gatgtcgccc
    10861 tgatctgcgt ggggactccg gacatcggac acggacagca ggatctgacg gcgctgtcgc
    10921 acgtcgcggc cgccatcggc accgcgtgtc tcggtcgctc gcggcccttg acggtcatcc
    10981 tgcggagcac ggtgctgccc ggtacgaccg ctcgcgtgct ccagcgcatc ctgatcgaca
    11041 ccgcgcagca ctactcttcg acggttcaca ttgccgtcaa cccggagttc atgcgcgagg
    11101 ggtcctccct cgaggacttc cagaatcctc cgttcatcct ggctggctgt gatgacgacg
    11161 agacagcgag cgtgctgcgc cgactctatg ccggcgtgga tgcaccgttt ctccagacca
    11221 ccgtgcgaac cgcggagatg gtcaagtacg tctgcaacgc ctatcacgcg ctcaagatct
    11281 gcttcgcgaa cgaagtcggc gatctctgtg acgcgctcta cacggacggg caggaggtca
    11341 tgcggatcgt ctgcgaggat cgcaagctca acatctcggc cgcctatctc acgcccgggt
    11401 tcgcgttcgg cggatcgtgt ctgccgaagg acgtgcgggc gctgctgtcc gccggccgca
    11461 tcgcggacat cccgctgccg ttgctgggcg cgatcgtctc gtcgaacgac ggcctcatcc
    11521 gacgtggtgt ggacgccgtg ctccgcacca gacgacgccg tgtcggcgtc gtcggactgg
    11581 ccttcaagcc caccacagac gacctgcgcg agagcccgat ggtcgcactc gtcgaggcgc
    11641 tcatcgggaa gggctgcgac gtgcgcatct acgacccgac cgtcgtgctg gcgtccctga
    11701 agggagccaa ccgccggtac atcgaggccg agattccaca catctcgtcg ctgctgtgcg
    11761 acagtgtgga cgcactcatc acgcactccg acgtgctcgt gttcggcgcg acgggcgtgc
    11821 aggcggagcg ggtgatggcc gccgcgaccg accgccaggc gatcgtggat ctcactcatg
    11881 gggccctcgt caggcacgtg gagcgggctt cggatctcgt cgggctcgag gttgagccat
    11941 gacggcccgc acattcgtcg cgtgcggcgt cgccccggtg atcctgctgg cgatggtggc
    12001 ggcgacccgg cccgaagcgg tgcgtcccgc cggctcgccg gggcctgctg attcgagggg
    12061 cgcgcttcag ggcgctctgc ccgaggggcc cagggagtcg gtggacgccg acacgatccc
    12121 cgaaccgaag cggcgcatcc aagtgtccgc atcgggagat cttcaacgag cgctcgacca
    12181 ggcgcagccg ggcgaccaga tcgagctgga gaacggcgcg acgtatcagg gtcccttccg
    12241 ccttcgcgac aaacagggtg atggctggat cgtcgtgacg tcggcggatc cgcgcggcct
    12301 tcccgcaccc ggcgagcgcg tcagtccgtc gcacgcgccg cggatggcga agctgcgtgc
    12361 ctcacacgga gccgtgattg cggccgacgc gggtgcgcat cattaccggt tcatcggcct
    12421 cgagatcgcg ccgagcgccg gcac
//
LOCUS       DBNGSH010000404         4346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2413621, whole genome shotgun sequence.
ACCESSION   DBNGSH010000404 DBNGSH010000000
VERSION     DBNGSH010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2413621"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="AC16CY_09300"
     CDS             <1..495
                     /locus_tag="AC16CY_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354025.1"
                     /translation="TMYRPAGNLNQLMRGVFFPNSNLIFSIQEVEAADLPPPPPASRP
                     DGLTTFDWGLLIYKGWPVVENSATALADASMLLLQPGLRCENGRAAPITEPDWIRFTD
                     RMIAVAKRTYRLAQTKNKDAVSEYTADLSNACNACHGTYRADGGRGRGRGGNLSTAGR
                     CMHR"
     gene            572..931
                     /locus_tag="AC16CY_09305"
     CDS             572..931
                     /locus_tag="AC16CY_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3354026.1"
                     /translation="MAVDQLSSTFAALADPTRRAILARLASGECSVTELAEPFDMSMP
                     AVSKHLRVLERAGLIARGREAQWRPCRIEAGPLKHVADWTERYRHLWEARFDRLEHYL
                     QGMKAKEKTHGRKQRGK"
     gene            906..1406
                     /locus_tag="AC16CY_09310"
     CDS             906..1406
                     /locus_tag="AC16CY_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3354027.1"
                     /translation="MAVSSVANSDTFKVTTPSDREITLTRLFDAPRRLVFEALTRPEH
                     VKRWWGILDDQYSVPVCEMDLRVGGKWRIVGRGPHGEGPEFYGEYLEIVPPERLVNTE
                     IFAPFPDAGSVVTVVLTEEDGKTRLTVTAAYPSREVRDMVLQTGMETGAALSYDRLEE
                     VARELR"
     gene            1423..1497
                     /locus_tag="AC16CY_09315"
     tRNA            1423..1497
                     /locus_tag="AC16CY_09315"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1455..1457,aa:His,seq:gtg)
     gene            complement(1522..4113)
                     /locus_tag="AC16CY_09320"
     CDS             complement(1522..4113)
                     /locus_tag="AC16CY_09320"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3354028.1"
                     /translation="MPLSNGTRLGSYEVTGRIGTGGMGEVYRARDTKLGRDVAIKVLP
                     AALADNPQRLARFQQEARVLAALNHANIAHIYGIEQGALVMELVEGKTLDGPVPAATA
                     IAYARQIADALEAAHEKGIVHRDLKPGNVMVTANGVIKVLDFGLAITPEATQDPDNSP
                     TVAVPLTEAGMVMGTAAYMSPEQASGKPVDKRADIWAFGVLLCELLTGRKLFGGETTS
                     HVLAAVLTQEPDLTAVPASLRPVIEGCLRKDPRARWHDITDVRMLLELRREDPVQSAA
                     AGRAWIPWVVAAVAIIAAIASVFLRTPPAAADRPFAQVAVEVGAQVAQPALSPDGSRI
                     VFVSGGRLAMRRLDQTEMTALPGTEGASFPFFSPNGEWVGFYAAAKLKKIAVAGGVAQ
                     SIGDVPSPRGGSWGADDVIITSRETLDGLWQFPANGGEPKRLTTPAGDPEGMTAHRWP
                     QILPDRKGVLFSATAGLRGSLRVLLPDGTVKSLLSNAPYGRYSTTGHLIYYQSGSLFA
                     ARFDLDRLEITGPAIPLVQGVAFTPNVANAEFDLSPDGTLVYVKGPASTDRVISWVET
                     SGTVTPLLAKPREYAYVAVSPDGKRVAAIFQEDGRSDLWVYDPARDLLTKLNSGDRAI
                     WPVWTPQGDFILTGNGPLIRWSRSDGGAGGEAGPKAESGVFNLGSFSKAGDRFAYSAS
                     AEGGRSWVVSVERTPEGLKFGEPEALLDGLVPALSPDGRFVAYGSRESGRAEIYVRPL
                     LTTPNGIHVAASGRQQISSEGGSYPQWSSSGHELFYQGSDRRIWVATYTVAGDQLLPK
                     KARHWSDVQISDLGSIPSFSLAPDGKRVAAILDRDQAPTPETRIHVLFNLPDELRRRE
                     ASATPSR"
     gene            complement(4160..>4346)
                     /locus_tag="AC16CY_09325"
     CDS             complement(4160..>4346)
                     /locus_tag="AC16CY_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR3354029.1"
                     /translation="FWKYLGVLSLTELPYSVATIYLGAGFVERRITLLIVVGALMVSF
                     SGLALHALHRRFREQRG"
BASE COUNT          783 a         1441 c         1370 g          752 t
ORIGIN      
        1 acgatgtacc gtcccgccgg caacctcaat cagttgatgc gcggcgtctt ctttccgaat
       61 tcgaatctga tcttctccat tcaggaggtg gaggcagcag acctgccgcc gccgcccccg
      121 gcctcgaggc ccgacgggct gacgacgttc gattggggcc tgttgatcta caagggatgg
      181 ccggtggttg aaaactccgc caccgcgctc gccgatgcat cgatgctgtt gcttcagccc
      241 ggccttcgct gcgaaaacgg ccgggccgcg ccgatcaccg agccagactg gattcgattc
      301 accgatcgca tgatcgccgt cgcgaagcgg acgtaccggc tcgcgcagac gaagaacaag
      361 gacgccgtct cggagtacac cgccgatctc tcgaatgcct gcaacgcctg ccatgggacg
      421 tatcgcgccg acggcgggcg cggacgcggg cgcggcggca acctgagcac tgccggacgc
      481 tgcatgcatc ggtaatcgcg ccgggcttga ctcggacatc ggagccgttg tattaaacct
      541 atgggttaat taactcatag gttcactaca catggccgtc gatcagctca gctccacttt
      601 tgccgccctc gccgacccca cccgccgggc catcctcgca cggctggcct ccggcgagtg
      661 ctccgtcacc gagctcgccg agcccttcga catgagcatg cccgccgtct ccaagcacct
      721 ccgcgtgctc gagcgcgcgg gtctcatcgc acggggacgc gaggcccagt ggcgcccctg
      781 ccggatcgag gccggacccc tcaagcacgt ggccgactgg accgagcgct atcgccacct
      841 ctgggaagcg cgtttcgatc ggctcgaaca ctacctgcaa ggaatgaaag cgaaggagaa
      901 gacccatggc cgtaagcagc gtggcaaata gcgacacctt caaggtcacg accccgtccg
      961 atcgcgagat caccctgacg cgcctcttcg acgcccccag gcggctcgtg ttcgaggcgc
     1021 tcaccaggcc cgagcacgtc aagcggtggt ggggcatcct cgacgaccag tactccgtgc
     1081 ccgtgtgcga gatggacctc cgcgtcggcg gcaagtggcg catcgtcggg cgaggcccgc
     1141 acggcgaggg ccccgagttc tacggcgagt acctcgagat cgtcccgccc gaacgcctgg
     1201 tcaacacgga gatcttcgcg ccgttccccg acgccgggtc ggtcgtcacc gtggtgctga
     1261 ccgaagagga cggcaagacg cgcctcaccg tcaccgccgc ctacccgtcg cgcgaggtgc
     1321 gcgacatggt gctccagact ggcatggaga cgggcgccgc cctcagctac gaccgtctcg
     1381 aggaggtggc gcgggagctg cggtaaactt gaaggctcgg tggtgggtgt agctcagggg
     1441 tagagcgcct gactgtggct caggaagtcg cgggttcgaa acccgtcacc caccccactc
     1501 ttccttcaca ttgctcgatt gtcacctgga cggcgtcgcg gatgcctcgc ggcggcggag
     1561 ttcgtcaggc aagttgaaga gcacgtgaat gcgggtctcg ggcgtggggg cctgatcacg
     1621 atccaggatg gctgcgaccc gctttccgtc cggtgcgagc gagaacgacg ggatggagcc
     1681 gagatccgaa atctgcacgt cggaccagtg gcgcgccttc ttcggcaaca actggtcacc
     1741 ggcgactgta taggtcgcga cccagatgcg ccggtcggag ccctgataga acagctcgtg
     1801 tccggaggat gaccactggg gatagcttcc cccctcgctc gatatctgtt gtcttccgga
     1861 agccgcaacg tggattccgt tcggcgtggt cagtaacggc ctgacgtaaa tctcggcccg
     1921 accggattcc cgagatccat aggcgacgaa ccgtccgtcc ggcgaaagag cagggacgag
     1981 cccgtccagc agggcctcgg gttctccgaa cttcagccct tccggagttc gctcgaccga
     2041 cacgacccac gatcgccccc cctcggcgga agcggaatag gcgaaccggt ctccggcctt
     2101 cgagaacgac cccaggttga atacgccgga ttccgccttc ggtcccgcct ctcctccggc
     2161 gcccccgtcg gagcgcgacc agcggatcaa ggggccattc cccgtgagga tgaagtcacc
     2221 ttgcggggtc cagacaggcc aaatcgccct gtcgccggaa ttgagcttcg tcaacagatc
     2281 ccgggcggga tcgtagaccc acaggtcgct tcttccgtcc tcctggaata tcgccgcgac
     2341 tcgctttccg tcgggggaga ccgcaacata tgcatactcc ctcggcttgg ccagaagcgg
     2401 cgtcaccgtg ccggacgtct ccacccagga aatcacgcga tcggtagacg cgggtccttt
     2461 cacatacacg agcgtgccgt caggtgagag atcgaactcc gcattcgcga cattcggggt
     2521 gaatgcgacg ccctgcacca agggaatcgc ggggcccgtg atctccaggc gatcgagatc
     2581 gaaccgtgcg gcaaacaacg atccggattg gtagtagatc aggtgaccgg tcgtggagta
     2641 acggccgtag ggagcgttcg agaggaggct tttgaccgtt ccgtcgggaa gcaatacccg
     2701 gagcgatccc cgtaaacccg ccgtcgctga aaagagcact ccttttctgt cgggcaagat
     2761 ctgtggccag cggtgcgcgg tcattccttc gggatcaccc gcgggagtcg tgaggcgctt
     2821 cggttcgccg ccgtttgcgg gaaactgcca cagtccgtcg agagtttcgc gggatgtgat
     2881 gatcacatcg tcggcgcccc agcttccccc gcggggggac gggacatcgc cgatggactg
     2941 cgctacgcct ccagcgacgg cgattttttt gagcttggcc gcggcgtaga aaccgaccca
     3001 ctcgccgttg ggcgagaaga acgggaagga tgcgccttcg gttccgggga gagccgtcat
     3061 ctccgtttga tccaggcgcc tcatcgccaa cctgcccccg gacacgaaga cgatccgcga
     3121 tccgtctgga gacagcgccg gctgcgcgac ttgcgcgccg acctccacag caacctgcgc
     3181 aaagggacgg tcggcggccg cgggtggagt gcgcaggaat accgacgcaa ttgctgcaat
     3241 gattgccacc gcggcgacga cccatggaat ccaggcacgc cctgcagccg ccgattgaac
     3301 cggatcctcc ctgcgaagtt cgagcagcat cctgacgtcc gtgatgtcat gccagcgggc
     3361 gcggggatcc ttccgcaggc agccttcgat cacgggacgg agactcgccg ggaccgctgt
     3421 caggtcaggt tcctgcgtga gcacggcggc caggacatga gacgtcgtct cgccgccaaa
     3481 cagcttcctc cccgtcagga gctcacacag cagcacgccg aaggcccaga tgtcggcgcg
     3541 cttgtccacg ggtttgccgg acgcctgctc cggagacatg tacgctgcgg tgcccatcac
     3601 catgcccgct tccgtcagag ggaccgcgac tgtgggtgag ttgtccggat cctgcgtcgc
     3661 ctccggcgtg atggcgaggc cgaagtcgag aaccttgatg acgccgttcg cggtcaccat
     3721 gacgtttccc ggcttcaagt cccgatgcac gatgcccttt tcgtgcgcgg cctccagtgc
     3781 gtccgcgatc tgccgcgcat acgcaatcgc cgtggccgca ggcacggggc cgtcgagggt
     3841 ctttccctcg accaattcca tcaccagggc gccctgctcg ataccgtaga tgtgcgcgat
     3901 gttggcgtgg ttcagcgccg ccagaactct ggcttcctgc tggaaccgtg cgagcctctg
     3961 cggattatcg gcgagtgcgg cagggaggac cttgatcgcg acgtcgcgcc ctaacttcgt
     4021 atctcgcgcg cggtacacct cacccatccc gccggtgccg attcgacccg tgacttcgta
     4081 gcttcccagc cttgtgccgt tggaaagcgg catattgagc gtcagcgcag tctatacgat
     4141 tgaggagccg aaggacttct cagccccgct gctcccgaaa ccgccgatgg agcgcgtgca
     4201 gcgccaggcc gctgaaggag accatcagtg cgccgacgac gatgagcagg gtgatcctgc
     4261 gctccacgaa tccggcgccg agatagatcg tcgccactga gtacggcagt tcggtcagag
     4321 aaagcacgcc gaggtatttc cagaag
//
LOCUS       DBNGSH010000405         2360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2583420, whole genome shotgun sequence.
ACCESSION   DBNGSH010000405 DBNGSH010000000
VERSION     DBNGSH010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2583420"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="AC16CY_09330"
     CDS             complement(<1..978)
                     /locus_tag="AC16CY_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3354030.1"
                     /translation="MSRPGAVAFALAAAIALGGCSPSSGTSAATRRLSIATGGTGGVF
                     YPYGGGIAKIITEHLDHVEATAEVTAASVDNLKFLEQGTSDLAFTMADMAQDALLARD
                     VFTDFGAVRVRTLAVLYSSYSHLVTTADSGITQIAHLRGRVVSTGAAGSGTTVLARRM
                     LRAAGLDPSRDIREQSLGAAQSADALKDGKIDAFFWNGGLPTGAILELVNTPGVTARF
                     VPTAGQLPDLERAFGPSLYYREIIPKAVYGTDEDIPVVGVANLLVASETMPEALAHDI
                     TQLLFDRQPELAAIHPQAKSLSLSTALTGSPIPFHPGAVRFYRERQVWKD"
     gene            997..1923
                     /locus_tag="AC16CY_09335"
     CDS             997..1923
                     /locus_tag="AC16CY_09335"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR3354031.1"
                     /translation="MADLVSLDDIRAAATRIRAIVRRTPMVEVRGLFLKCENLQPGGA
                     FKIRGACNMIAQLPPGARAAGVITYSSGNHGTAVALVADTLGIPAVVVMPENAARIKV
                     EKVRRYGAEVFFAGSTSDDRKARAEELAHQRGLTIVPPFDHPWIIAGQGTTGLEILEQ
                     LPAVRAIYVPVGGGGQIAGISAAVKRMRAHVRVIGVEPLHAARMARSIGAGHPVTLDK
                     AVSIADGLLTLRPGDLTFAHVRELVDDVVSIDDESIAGAVRWLFTEAKLVAEPSGAAA
                     VAAALRAGEGANVVAVVSGGNVDARDFAEYVR"
BASE COUNT          390 a          764 c          818 g          388 t
ORIGIN      
        1 atccttccac acctgtcgtt cgcggtagaa gcgcacggcg ccgggatgga aggggatggg
       61 agagccggtg agtgccgtcg agagagagag cgacttggcc tgcgggtgaa tcgccgcgag
      121 ctcgggctgc cggtcgaaca gcagttgtgt gatgtcgtgc gcgagcgcct cgggcatcgt
      181 ctcggacgcg acgagcaggt tcgcgacccc gaccaccggg atgtcctcgt cggtgccgta
      241 gaccgccttg ggaatgatct cgcggtagta caaggagggc ccgaacgcgc gctcgagatc
      301 gggaagctga ccggccgtcg gcacgaagcg cgcggtcaca ccgggcgtgt tgaccagctc
      361 gaggatcgct ccggtcggca atcccccgtt ccagaagaac gcgtcgatct ttccgtcctt
      421 cagcgcgtcc gccgactgtg cggcgccaag gctctgttcg cggatgtcgc gcgagggatc
      481 gagccctgcg gcccgaagca tcctgcgggc gagcaccgtc gtgcccgaac cggccgcgcc
      541 ggtcgagacc acccgtcccc gcaaatgggc aatctgggtg atgccggagt cggccgtcgt
      601 caccagatgc gaatagctcg aatagagcac cgcgagcgtg cgcacgcgca ccgcgccgaa
      661 atcggtgaag acatcccgcg cgagcagcgc gtcctgggcc atgtcggcca tcgtgaacgc
      721 gaggtccgac gtcccctgtt cgaggaactt caagttgtcg accgatgccg ccgtgacctc
      781 ggcggtcgct tcgacgtggt cgaggtgctc cgtgatgatc ttcgcgatgc cgccgccata
      841 tgggtagaag acgccgccgg tgcccccggt cgcaatcgac agacggcgcg tagccgccga
      901 cgtacccgag gacggactgc agcctcccaa cgcgatcgcc gccgccagcg cgaacgcgac
      961 cgcgcctggt cttgacatgc tgcacactat atccggttgg ctgacctcgt atcgctcgac
     1021 gacatcaggg cggcggcgac ccgcatccgc gcgatcgtgc ggcgtacgcc gatggtcgag
     1081 gtcagaggtc tgttcctcaa gtgcgaaaac ctccagccgg gaggcgcgtt caagattcgc
     1141 ggcgcgtgca acatgatcgc gcagcttccc cccggggcga gggcggccgg cgtcatcacc
     1201 tactcgtccg ggaatcatgg aacagccgtc gcactcgtcg ccgacactct cggcattccg
     1261 gccgtggtcg tcatgcccga gaacgcggca cgcatcaagg tcgagaaggt gcggcggtac
     1321 ggcgccgagg tgtttttcgc gggatcgacg tccgacgatc gaaaggcgcg ggccgaagaa
     1381 ctggcgcatc agcgcggcct cacgatcgtt ccgccgttcg atcacccctg gatcatcgcc
     1441 ggacagggga caacgggcct tgagatcctc gaacagctcc cagcggtgcg cgccatttac
     1501 gtcccggttg gcggcggcgg ccagatcgca ggcatcagcg cggcggtgaa gcgcatgcgg
     1561 gcgcacgtgc gtgtgatcgg cgtcgagccg cttcacgcag cccggatggc gcggtccatt
     1621 ggcgcaggcc atccggtcac gctcgacaag gcggtcagca ttgctgatgg ccttctgacc
     1681 ctgcgtcctg gcgacctgac gttcgcgcac gttcgcgagc tggtggacga cgtcgtgtcc
     1741 attgacgacg agtcgatcgc gggcgcggtg cgatggctgt ttacggaagc gaagctggtg
     1801 gccgagccga gcggcgctgc ggccgtggcg gcggcgcttc gagctggcga gggagccaac
     1861 gtcgtggcgg tcgtctccgg cggcaacgtg gacgctcggg acttcgccga gtatgtccgt
     1921 taaactgacc tctcctcgcg caggctgaag cctgcgcgct acgcgtgtta ggtagatcgg
     1981 gggcttcagc ccccgcggca gcgacgcatg ttacgtagag cgggggcttc agcccgcgca
     2041 gcagctacgc atgttacgta gagccggggg cttcagcccg cgcagcagcg acgcatgtta
     2101 cgtagagcgg gggcttcagc ccgcgcagca gctacgcatg ttacgtagag cgggggcttc
     2161 agcccccgcg acagcgaccg gagcccggaa ccgccgggac gtccgcctcg tcacatggag
     2221 ttgaatccta tgagaagcac ggtgcacgcg ctgggttttc tcgtcaaagg gcccatcatc
     2281 ctggcgtttc tcttcctcat caacctgatg acgggagggc cgtggtgggt gcaatggccc
     2341 gcgctcggca tcggcatcgc
//
LOCUS       DBNGSH010000406         4135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3602284, whole genome shotgun sequence.
ACCESSION   DBNGSH010000406 DBNGSH010000000
VERSION     DBNGSH010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3602284"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="AC16CY_09340"
     CDS             complement(<1..1237)
                     /locus_tag="AC16CY_09340"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3354032.1"
                     /translation="MTRLLSRLGLGRPELRAWALYDCANSAFWTTIITSVFPPFFSDH
                     ATAGAPDATARFAWTTTIAVTIVALIAPVLGAMADYRAIKKRMLAACVAIGVVATALM
                     STIARGDWIYAAVLFVVGNIAVATSLVFYDSLLPHIAAPDELDRVSTSGYAIGFLGGG
                     LLLLANLAWILSPATFGLPNTLAAIKLSFVSVAIWWLAFTIPLLRRVREPKVVLEADE
                     TGLENPVRIAVIRVWEAFHRLRTFPNAFLMLVAFLLYNDGIQTMVRMSSIYAAEVGID
                     RNAQIAAYVLVQFIGVPCTFLFGAAAGRIGAKPALFGALVVYVGISCLGYFMTATWQF
                     FALATLVGMVQGGSQALSRSLFARMIPKDKSSEYFGFFAVFEKFAHIAGPTLFAVSVT
                     LFHSSRAAVLSVIVFFVLGA"
     gene            complement(1234..2379)
                     /locus_tag="AC16CY_09345"
     CDS             complement(1234..2379)
                     /locus_tag="AC16CY_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3354033.1"
                     /translation="MQNPFVYGEIVPGEAFVDREVELDRLIGDLAAGQKVFLISPRRY
                     GKSSLIRQALAALVRRGAATVDVTVSSYSSYLSFLEGYARALAAVETKWERARGWLAK
                     AISSTRPEVRYDAEGPGPGRVSVAFPLVQSARDVNRLATEVFSLPGRLATEQKRLVVV
                     ALDEFQAIEGFDGGTVEHALRAAAERQRRVGYVFAGSEPSLMDKMIGPRRPFYKAGPV
                     MRLQKIPADVFAGAIEARFGRSGIRAEAGLGAAIVDLAGNLPYDVQRLAHETWDDVRA
                     AGARKATLEALHATLARLLDEQDTMFEAVWQRLTLAQRGVLRAVVLHVGQGLLSTDVR
                     TRYRLGGPSSIQTSLAALVKQDLVLREGGQYVVVDSLLREWIARRTF"
     gene            3600..>4135
                     /locus_tag="AC16CY_09350"
     CDS             3600..>4135
                     /locus_tag="AC16CY_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354034.1"
                     /translation="MVRLTITVCAALLAVLAFDETALLGQAQDDGVLSINLEPEAAED
                     RGTLPRSYRIYGEPHILRIRFSLSSQSDAPIVVDQAAMQKLFRIRLQAGSEIPVVGQW
                     APEIYLSGELFWIQLTNSFTPLLIEPQTGVGWTITLERADGMAFARGEYVLEYEIPGL
                     QSTIRKADDTPWIGRAPSV"
BASE COUNT          754 a         1336 c         1375 g          670 t
ORIGIN      
        1 gcgcgccaag aacgaagaac acgatgactg aaagcacggc tgcgcggctg gaatggaaca
       61 gcgtcacaga cacggcaaag agcgttggac ccgcgatgtg ggcgaacttc tcgaaaaccg
      121 cgaagaagcc gaagtactcc gacgacttgt ccttcgggat catgcgggcg aacagtgacc
      181 ggctgagcgc ctggctgcct ccctgcacca tgcccaccag cgtcgccagc gcgaagaact
      241 gccacgttgc ggtcatgaag tagccgaggc acgaaatgcc gacgtacacg acgagcgctc
      301 cgaacaaggc cggcttcgcg ccgatgcggc ccgcggctgc gccaaacagg aacgtgcacg
      361 gcacaccgat gaattgcacg agcacgtacg ccgcgatctg cgcattccgg tcgatgccga
      421 cttcggcggc atagatcgag gacatccgga ccatggtctg aatgccgtcg ttgtacagca
      481 ggaacgctac gagcatcagg aaggcgttcg ggaacgtgcg caggcggtga aacgcctccc
      541 agacgcggat cacggcgatg cggacggggt tttccaggcc ggtctcgtcg gcctcgagca
      601 caaccttcgg ctctctcacg cggcggagca gcgggatcgt gaaggcgagc caccagatgg
      661 ccacgctgac gaaggacagc ttgatcgcgg cgagggtgtt cggcaggccg aacgtcgcgg
      721 ggctcaggat ccaggcgagg ttggccaaca gcagcaaccc tccgcccagg aagccgatgg
      781 cgtagccgga cgtcgagacg cggtccagtt catcgggggc agcgatgtgg ggcagcagcg
      841 aatcgtagaa gaccaggctc gtcgccacgg cgatgttgcc cacgacgaac agcactgccg
      901 cgtagatcca gtcgccgcgt gcgatggtgg acatgagggc cgtggcgacc acaccgatcg
      961 cgacgcacgc ggcgagcatc cgcttcttga tcgcccggta gtcggccatc gccccgagca
     1021 ccggcgcgat gagcgcgacg atggtgacgg caatggtggt cgtccacgcg aatcgcgccg
     1081 tcgcatcggg ggcgcctgcg gtcgcatggt cgctgaaaaa cggaggaaag accgaagtaa
     1141 tgatggtcgt ccagaaggcg gagttcgcgc agtcgtacag cgcccacgcg cgcagctccg
     1201 gccggcccag gccgagacgc gagagcagac gggtcaaaac gttcgcctgg cgatccattc
     1261 gcggagcagc gagtccacca cgacgtactg gccgccctcg cggagcacga ggtcctgctt
     1321 gacgagcgcg gcaagcgagg tctggatgct cgacggtccg ccgagccggt agcgggtccg
     1381 gacgtcggtc gagagcaggc cctggccgac gtgcagcacc acggcgcgaa gcacaccccg
     1441 ttgagcgagc gtcagacgct gccagacggc ttcgaacatc gtgtcctgtt cgtcaagcag
     1501 ccgcgccagc gtcgcgtgca gcgcctcgag cgtggccttg cgcgcccccg ccgcgcggac
     1561 gtcgtcccac gtttcgtgag ccaggcgctg cacgtcgtac ggaagattgc ccgcgagatc
     1621 gacgatggcg gcgccgaggc cggcctcggc gcgaatgccg cttcgtccga acctcgcctc
     1681 gatggccccc gcaaagacgt cggcggggat cttctgcagg cgcatcaccg gacccgcctt
     1741 gtagaagggg cggcgcggac cgatcatctt gtccatcagg ctcggctccg agccggcgaa
     1801 cacgtatccg acccggcgct ggcgctcggc tgcggccctc agcgcgtgct cgacggttcc
     1861 tccgtcgaag ccctcgatgg cctgaaactc gtcgagcgcg acgacgacga ggcgtttctg
     1921 ctccgtcgcc aggcgtccgg gcagcgagaa gacctcggta gcaagacggt tcacgtcccg
     1981 cgcgctctgc acgagcggga aggccaccga gacgcggccg ggccccggcc cctcggcgtc
     2041 gtagcggacc tcggggcgcg tggacgagat ggctttcgcc agccagccgc gggcgcgctc
     2101 ccatttcgtc tccaccgcgg cgagcgcccg cgcgtagcct tcgagaaacg agaggtacga
     2161 gctgtaactg ctgacggtca cgtcgaccgt cgcggcgccg cggcggacca gcgcggccag
     2221 cgcctgccgg atgagggatg acttgccgta ccggcgcgga gagatcagga agaccttctg
     2281 gccggcggcc aggtcgccga tcaggcggtc gagctcgacc tcccggtcga cgaaggcctc
     2341 gccgggcacg atctcgccgt agacgaacgg gttctgcacg gcagtacgtg aagtggcgta
     2401 acggttacgt cgatgagcgt aacacaagct tgttggtgga gggtgattcg ttgttgctct
     2461 gcccggccgt cggctgccgc tgccgcctga tccacaccgg agctcagtcg tgtttgagac
     2521 ggcgaatccc ggtttccgcc tccgccaagg ctacggcgag acctcgccga agcgcgttgc
     2581 gcgaaggcgg gccggtggcc ggaccgctct ccaccccaac gcgcaggcgt gcgcgttggg
     2641 gaccccagcg ctgcgagggc gtcgccggcc gcgtgacgct cgggtcgcgc gtgctctcgc
     2701 acacgctcaa cctcgcggaa ccccccagcc ttctcgcgcg gtcaggcgac tggagtcggc
     2761 ctgacctcca caggaattcg ccatccaaac acgactgagc tccggtatgc cggggtacaa
     2821 cgtggggtgt ggcttcccgt ccccggtgtg ccgaggatcg attcagaata acttcgaagg
     2881 ataaggaccc aaagcgatgc ctacgcgcct ggttcttggg atcaccaggg atcacctgtg
     2941 tcgagcctga tctgccggaa cacgtgcggg gcgagtcggc cctgcgtatt ccgtccctgc
     3001 gttccaggcg caggcctcag cgcgttgttt tgagggtgtg actctcatcc atcagacacg
     3061 agttcgccgg ctagccgcgt ctgggctggc gccgttcgac ccggcgctcc ggtcggacgt
     3121 cgtggaaaac cgagccctcc acacggtgtt cagcgaccgg cgcagctccg tgagctgcgc
     3181 ggcggtcacg tttcgggccc ggtttacccc ttcgcgtgtc acgacgccga cagtcgcgcc
     3241 tctcgaacgg cacctgccca gacgcagctg gccggcgaaa cccgggctca cagtactcgc
     3301 ctgacatcac aattgacgtg tgcgtacgtc agttcctggg tccgcaaagc agatgaattc
     3361 acaggcccaa atcgacaacg agatcaggcg aaccatcgca tacgaggttg ggcacggaat
     3421 ccacgttaag catcgagggg gacccaacat ttgcaacggt cgtgcgacag ttggctcggt
     3481 caacagcgtc gtgagctggg gttggaagag tgggccttgg tccactagcg ccatagcgac
     3541 gtatgatcct gtgttcgacg tgccgcagat tcgcctacgt gtcccatgag ggtgaaacta
     3601 tggttcggct gacgatcacc gtctgtgctg ccctgctagc ggtccttgca ttcgatgaga
     3661 cggctctcct cggtcaggcc caggatgacg gtgtgttgtc gattaacctc gaacctgagg
     3721 cggcagaaga tcgcggcacc cttccgagat cgtaccggat atacggcgag ccccacatcc
     3781 ttcggatcag gttcagcctg tccagtcagt ccgacgcgcc gattgttgtc gatcaggccg
     3841 cgatgcagaa actctttcgc atccgcctcc aggcaggaag cgagattcca gtggtcggcc
     3901 agtgggctcc cgagatctat ctgtcgggcg aactgttttg gattcaactc acgaattcgt
     3961 tcaccccact gctgatcgag cctcagacag gtgtggggtg gacgattacg ctcgagcgcg
     4021 ctgacggcat ggcattcgcg cgtggcgagt atgtgctgga gtacgagatt ccgggcctgc
     4081 agtccacaat ccggaaggcc gacgatacgc cctggatcgg tcgagcaccc tccgt
//
LOCUS       DBNGSH010000407         3072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1565355, whole genome shotgun sequence.
ACCESSION   DBNGSH010000407 DBNGSH010000000
VERSION     DBNGSH010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1565355"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..2116)
                     /locus_tag="AC16CY_09355"
     CDS             complement(182..2116)
                     /locus_tag="AC16CY_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3354035.1"
                     /translation="MSEKRSVDRRGFLKGAAVTGAAAFAAGSAEAAALQQAAPDSRAT
                     APVAAREADPPASVEVQTIDRPGGDFMVDVIKTLDLEYVASNPGSSFRGIHESIVNYG
                     GNRLPEFITCCHEESSVAMAHGYFKAEGKPMAVLCHGTVGMQHAAMAIYNAYCDRVPV
                     YILAGNSLDATLRRPGVEWAHSVQDAAAMVRDYIKWDDQPISLPHFAESAIRAYKIAM
                     TPPMMPVVLVIDGGLQEDPISHEVTPRLRIPKLTTATPPQGDSGAVAEAARLLVNAQN
                     PLIIADRAARTPAGLTRLVELAETLQAPVVDQGGRMNFPSRHPLNQSSSARQLVANAD
                     VILGLELTDFWGTVNAVRDGVTRTSRSLARAGTKLVSITALDLATKGNYQDFQRYPEV
                     DVAMAADAEATLPALTEACRRLITPERRAVFQERGTRLAAAHAAALERARAEATYAWD
                     ASPISTARLTTEIWAQIKNEDWALVAESGIMSDWVSQLWAFDKHYQSLGGSGGAGVGY
                     GAPAAVGAALANKKRGRLSVTIQGDGDFLYAPGVWWTAAHHRIPLLAVMHNNRAYHQE
                     VMHLQRMANRHNRGITRAHIGTTIDDPNVDFAKIAQGLGVHAQGPITNPNDLAPALKR
                     AIDVVKRGEPSLVDVVTQPR"
     gene            complement(2335..2811)
                     /locus_tag="AC16CY_09360"
     CDS             complement(2335..2811)
                     /locus_tag="AC16CY_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3354036.1"
                     /translation="MAVSVFLDTSVLLAGLIDLGPQSAPAQSLMHAVAEKHVKDPATA
                     WHCCLEFFSVATRLPPEFRVSPSDAVLLLEEEVFARMAVHDLPSVDRLPMLRSAARDL
                     VAGGRVYDAHITEVARAAGAAVVVTDNRRHFLSALRYDMRVETPGEFLAGLRARRS"
     gene            complement(2802..3056)
                     /locus_tag="AC16CY_09365"
     CDS             complement(2802..3056)
                     /locus_tag="AC16CY_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3354037.1"
                     /translation="MRTTIDKAGRVVIPAGIRERAGLTAGTEIEVTEDELGVRLQRVA
                     AGPRLVKVGRRLVARPTVPRDARPSLDIATLVEEERNRWP"
BASE COUNT          506 a         1036 c         1007 g          523 t
ORIGIN      
        1 ccttgtgcct cgtagccgtg acactggtag cagccgtagc tgacgtacag cttcttccct
       61 gcttcggcat tcccggccgg cgccgccgcg ggttgcggcg acgacgtctg cgccgcgcca
      121 agcgccgccg atgcaagtaa cgcgaacaat gccgacagga gcaacagtcg atcctccccg
      181 actagcgtgg ttgcgtcacg acatcgacga gcgacggctc gcctcgcttg acgacgtcaa
      241 tcgcgcgctt gagcgccggc gccagatcgt tcgggttcgt gatcggcccc tgcgcgtgca
      301 caccgaggcc ctgcgcgatc ttcgcgaagt cgacattcgg atcgtcgatc gtcgtcccga
      361 tgtgcgcacg cgtgatcccg cggttgtgcc ggttggccat gcgctggagg tgcatgacct
      421 cctggtggta agcgcggttg ttgtgcatca cggcgagcag cggaatccgg tgatgcgccg
      481 ccgtccacca cacgccgggc gcatagagaa agtctccatc tccctggatc gtgaccgaca
      541 gccgcccgcg cttcttgttc gcaagcgccg caccgaccgc ggctggagca ccatacccga
      601 cacccgcgcc gcccgacccg ccaagcgact gatagtgctt gtcgaaggcc cacagctgcg
      661 acacccagtc gctcatgatg ccgctctcgg ccacgagcgc ccagtcttcg ttcttgatct
      721 gcgcccagat ctcggttgtc aggcgggccg tgctgatggg gctggcatcc catgcgtagg
      781 tggcctccgc ccgcgcgcgc tcgagcgcgg cggcgtgggc cgcggccaga cgcgtgccgc
      841 gctcctggaa taccgcgcgc ctctccggcg tgatgagacg cctgcacgcc tcggtgagcg
      901 ccggcagtgt ggcctcggcg tcggcggcca tcgcgacgtc gacctcggga tagcgttgga
      961 agtcctgata gttgcccttc gtcgccaggt cgagcgccgt gatgctcacg agcttcgtgc
     1021 cggcccgggc cagcgatctc gacgttcgag tgacgccgtc acggacggcg ttgaccgtac
     1081 cccagaagtc ggtcagctcc agcccaagga tgacgtcggc gttggccacg agctgccggg
     1141 cgctgctgct ctgattgagc ggatgccgcg acggaaaatt catccggccg ccctggtcga
     1201 cgacgggcgc ctggagcgtc tcggccagtt cgaccagccg cgtcaacccg gcaggcgtgc
     1261 gcgcggcgcg atcggcgatg atcagcgggt tctgcgcgtt gacgagcaac cgcgccgcct
     1321 cggccaccgc gccagaatcg ccctgcggcg gcgtcgccgt cgtcagcttc ggaatgcgaa
     1381 gacgaggcgt cacttcgtgc gagatcggat cctcctgcag ccctccgtcg atcacgagga
     1441 ccacgggcat catgggcggc gtcatcgcga tcttgtaggc gcggatcgcg gattcggcga
     1501 agtgcggcag cgagatcggc tgatcgtccc acttgatgta gtcgcggacc atggccgccg
     1561 cgtcctgcac gctgtgcgcc cactccacac ccggccgcct gagcgtcgcg tccagtgagt
     1621 tcccggccag gatgtagacc ggcacacggt cgcagtaggc gttgtagatc gccattgccg
     1681 cgtgctgcat gcccaccgtg ccgtgacaga gcacggccat cggcttgccc tcggccttga
     1741 agtagccgtg cgccatcgcc acagacgact cttcgtggca gcaggtgatg aactcgggca
     1801 ggcggttgcc accgtagttg acgatcgact cgtggatacc gcggaagctc gagccgggat
     1861 tcgatgcgac gtactcgaga tcgagcgtct tgatgacgtc gaccatgaag tcgccgccag
     1921 gcctgtcgat cgtctggacc tcgacgctcg ccggcgggtc ggcttcacgt gctgcgacag
     1981 gagccgtggc tcgactatcg ggcgccgcct gttgcagcgc agcggcctct gcgctgccgg
     2041 cagcgaatgc ggcggcaccg gtcacggcgg cgcccttgag aaagccacga cgatcgacgg
     2101 atcgcttctc tgacatcgcg tccctcgtcc acgttagcgc cgatagtcta acccactgtg
     2161 aggccaccgc ggacacaggt cattggccga ttcttgatct ttgattgttg aatcgttggt
     2221 gattggggat tgtcgattgg agatttattg tggggaatca ccaagagatc aacaagcaac
     2281 aatctgaaat caacaacgaa tcgacgatca caaatcccaa atcgacaatg cgtttcagga
     2341 ccgccgtgca cgaaggccgg cgaggaactc ccccggcgtc tcgacccgca tgtcgtaccg
     2401 cagcgcggag agaaagtggc gccggttgtc ggtcacgaca acggccgcgc cggcggcacg
     2461 ggcgacctcg gtgatgtgcg cgtcgtagac acgcccgccg gccacgaggt cgcgcgccgc
     2521 cgaccggagc atcggcaggc gatcgaccga gggcaggtcg tgaacggcca tcctggcaaa
     2581 cacttcttcc tcgagcagaa ggacggcgtc ggagggtgag acacggaatt cgggcggcag
     2641 ccgcgtggcg accgagaaga actcgagaca gcagtgccag gcggtcgccg ggtccttcac
     2701 gtgcttctcc gccacggcgt gcatgagcga ctgcgccggc gcgctttgcg gacccagatc
     2761 gatcagcccc gccagcagca cgctggtgtc gaggaagacg ctcacggcca ccggttgcgc
     2821 tcctcttcga cgagcgtcgc gatgtcgagc gacggccgcg cgtcgcgcgg gacggtcgga
     2881 cgtgccacca gccgccggcc gactttcacg agcctggggc cggcagccac tcgctgcagc
     2941 cgcacaccga gctcgtcttc ggtgacctcg atctcggtgc cggcggtcaa cccggcgcgc
     3001 tcccggatgc ctgcgggaat gaccacccgg cctgccttgt caatggtagt tcgcatacca
     3061 ttagactacc at
//
LOCUS       DBNGSH010000408         2639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3263302, whole genome shotgun sequence.
ACCESSION   DBNGSH010000408 DBNGSH010000000
VERSION     DBNGSH010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3263302"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="AC16CY_09370"
     CDS             complement(<1..502)
                     /locus_tag="AC16CY_09370"
                     /inference="COORDINATES: protein motif:HMM:NF016612.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HIR3354038.1"
                     /translation="MKRRSLVVLTIFLAAVVPRQAAGQRAERVPSLRAGAARVDITPA
                     QGEFPKNSRGILDRLYARAIVLENGASTAALITIDAGAVPDALWQAVTTQIEKELKIP
                     TASVLLTATHTHSAGGQRGPDYLQKIVESVRLAQQRLTPARVGYGTGVSYINVNRQII
                     DPKTNRW"
     gene            complement(555..1550)
                     /locus_tag="AC16CY_09375"
     CDS             complement(555..1550)
                     /locus_tag="AC16CY_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354039.1"
                     /translation="MKRRLLPAAPLFTAVMLMLPAQVPAPVTPTPEALVTAAKRAAGT
                     DHAGTFLRICVAPDNFNAGGRRGGGPGPRAVPDRATWYAKPYKVFDNLYFIGTRIHSA
                     WALTTRAGIIVIDTLFDYAIEPEMIEGMTTLGLDPRDVKYVLISHAHGDHDQGAALLQ
                     SRYGAKIVMGAADWDATLQRAASAPGGVPKRDIAVGREGLKLTLGEITVDVIATPGHT
                     SGTLSYFFPVKDAGRTLTVAYSGGTAFNFPRQAANFAIYRDSQRKMTEAAQAAGATVL
                     MSNHTEFDRAYDRARIAQLPRAVGEKHPYETDTATVMRYFEMAANCAEAQRLLVQ"
     gene            1669..>2639
                     /locus_tag="AC16CY_09380"
     CDS             1669..>2639
                     /locus_tag="AC16CY_09380"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3354040.1"
                     /translation="MKSLRVALAAGLAASLAMALQAEQGGGRAGGAATAGTGGALIGG
                     TLTPAKADARGWGWQVKASINSATPRPFYNKAKELLFQDKQITSYTIATFNPEFYCEV
                     ARHYDYIWFEMQHSTMSYDEVRRMILACPGVGAAPMIRMPDALESSIQKATDLGAIGI
                     IVPTVDDALEARDAARFSRYPPVGRRSSGGGSFGQVWPGVNYRATANDNMLVTVMIET
                     LEGVANAEEIAATHGVDALLVGNNDLSSFSGWSQNDPRYQDALVKVHDAALKYGKYFG
                     NAGAQYLNGYVLSADTRMVQNGPARDGWTPPARGGGPGRGTAPAGRGR"
BASE COUNT          435 a          850 c          906 g          448 t
ORIGIN      
        1 accagcgatt cgtcttcggg tcgatgatct ggcgattgac gttgatgtag gagacgcccg
       61 tgccgtatcc cactcgcgcc ggcgtcaggc gctgctgtgc gagccgcacc gattccacga
      121 tcttctgcaa ataatcgggc ccccgctgac cgccggcact gtgcgtgtgc gtggccgtca
      181 gcagaacact ggctgtcggg atcttcagtt ccttttcgat ctgcgtggtc accgcctgcc
      241 agagcgcgtc gggaacggca ccggcatcga tcgtgatcag cgccgcagtc gaggcgccgt
      301 tctcgagcac gatcgccctc gcatagagtc ggtcgagaat tccccgactg ttcttcggga
      361 actcgccctg cgcaggcgta atgtccaccc gggcggcccc ggcacgcagc gacggcacgc
      421 gctcggcgcg ctgccccgcg gcctggcgcg gcacgacggc cgcgagaaag atcgtcaaca
      481 ccacgagtga acggcgcttc atgacgcgga ttgtagcgcg gcgcgctgct gacgcgcgct
      541 gaacgcggcc ctgctcactg gaccagcagg cgctgtgctt cggcgcagtt cgccgccatc
      601 tcgaaatagc gcatgaccgt ggcggtatcg gtttcgtacg ggtgcttctc ccccaccgcg
      661 cgcggtagct gggcgatccg ggcgcggtcg taggcgcggt cgaactcggt gtgattggac
      721 atgaggacgg tcgcgccagc cgcctgggcg gcctcggtca tcttccgctg gctgtcgcga
      781 tagattgcga aattcgccgc ctgccggggg aagttgaacg ccgtgccgcc ggagtaggcg
      841 acggtcagcg ttcgtccggc gtctttgacc gggaagaagt acgacagcgt gcctgatgta
      901 tggcccggcg tggcaatgac gtccacggtg atctccccga gtgtgagctt cagcccttcg
      961 cggcccacgg cgatatcgcg tttcggcacg ccgccgggag cggatgcggc gcgctggagc
     1021 gtcgcatccc aatcggctgc acccatcacg atcttggcgc cgtagcggct ctgcagcagc
     1081 gccgcccctt gatcgtgatc gccgtgggca tggctgatca gcacgtactt gacgtcgcgc
     1141 ggatcgaggc cgagcgtcgt catgccctcg atcatctccg gctcgatcgc gtagtcgaac
     1201 agcgtatcga tgacgatgat gcctgctctc gtagtcagcg cccacgcgga gtggatcctg
     1261 gtgccgatga aatagaggtt gtcgaagact ttgtagggct tcgcgtacca ggtggcgcgg
     1321 tcgggcacgg cgcgtggccc tggccctccg cccctccggc cgcctgcatt gaaattgtcg
     1381 ggggcgacgc agatgcggag aaacgttccg gcgtggtcgg ttccggcggc tcgcttggcg
     1441 gccgtgacca gggcttcggg ggtgggggtg acaggcgcag gcacctgcgc tggcagcatc
     1501 aacatcaccg cggtgaacag cggcgcggcg gggagcaatc ggcgtttcat gccctcgatt
     1561 atgcgccgcc gagatgcatg gggcagcctg catctgccgg ttcgacgata atttgcgctg
     1621 agcagaaacg acgacaacga ctactatccg cacggagggg ggctggacat gaagtcactt
     1681 cgggttgcct tggctgctgg tctcgcggcg agtctcgcca tggcgctgca ggccgagcag
     1741 ggtggcggcc gcgccggtgg cgccgcgacg gcagggacgg gcggcgcatt gatcgggggg
     1801 acgctcacgc ccgccaaagc cgatgcgcgc ggctggggct ggcaggtgaa ggcctcgatc
     1861 aattcggcca cgccgcgccc gttctacaac aaggccaaag agctgctgtt ccaggacaag
     1921 cagatcacga gctacacgat cgccaccttc aacccggaat tctactgcga ggtcgccagg
     1981 cactacgact acatctggtt cgaaatgcag cacagcacga tgtcgtacga cgaggtgcgc
     2041 cggatgatcc tggcgtgccc gggcgtcggc gccgcgccga tgatccgtat gcccgacgca
     2101 ctcgagtcga gcatccagaa ggccacggac ctcggcgcca tcggcatcat cgtgccaacc
     2161 gttgacgatg cgctcgaggc acgcgacgcg gctcgattct cgcgctaccc gccggtcggc
     2221 cggcgaagct ccgggggcgg gtcgttcggc caggtgtggc cgggagtgaa ctaccgcgcc
     2281 accgccaacg acaacatgct cgtcaccgtc atgatcgaga cgctcgaagg tgtggccaac
     2341 gccgaggaaa tcgccgccac gcacggcgtg gacgcgctgc tcgttggcaa caacgacctc
     2401 tcgagcttct caggttggtc gcagaacgat ccccggtatc aggacgcgct ggtcaaggtg
     2461 cacgatgcgg cgctcaaata cggcaagtat ttcggcaacg ccggtgcgca gtacttgaac
     2521 ggctacgtcc tcagcgcgga tacgcgcatg gtccagaacg gaccggcacg cgatgggtgg
     2581 acgccgccgg cgcgcggtgg tggccccggg cgcggcaccg cgccggcagg acgcggtcg
//
LOCUS       DBNGSH010000409         4740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4282007, whole genome shotgun sequence.
ACCESSION   DBNGSH010000409 DBNGSH010000000
VERSION     DBNGSH010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4282007"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..293)
                     /locus_tag="AC16CY_09385"
     CDS             complement(150..293)
                     /locus_tag="AC16CY_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354041.1"
                     /translation="MRADHVWPQPGQSYQASTHVLSTVGTTHCPQSGQFIEAVAGSGL
                     GMP"
     gene            366..1310
                     /locus_tag="AC16CY_09390"
     CDS             366..1310
                     /locus_tag="AC16CY_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354042.1"
                     /translation="MTRTNPTPISESHPGPGSGEPPVVYWHRQLPPLEAEMIGEHILE
                     ATSGRVQGTLAHRDELWDQCYRSLIANTEERFAQEAARLGGHYAHVLDESIDSRRDDA
                     AGEAWLHGRFTYMLYRRATPDARHRVTAGLGTAVALVAGMALIPSMAVHAQKQHPPLE
                     TPEAVEASMKHFYRAANVIIVTTMDGMEHVYHFTRDLVVHGGKKPGVDALEGLREGTM
                     IVVHRNVSGPETSVAEIDLVGDEGLKITEGVVTDINRRKQEITIQYTNGTTETLKLTA
                     QAAAENDTLARSDSTRMRIVIYYADEAGRKVAHYIKGY"
     gene            1347..1874
                     /locus_tag="AC16CY_09395"
     CDS             1347..1874
                     /locus_tag="AC16CY_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354043.1"
                     /translation="MTRTSMHIGFVMIVGMWLIAGCGAPAPVEQQPAAAPPPPAAPVV
                     MSPVSINAEMVSIVDHAGHELWNVERQGRAPKARKDWENLAEHATQLAAAGALVRVAG
                     TGVNDVTWVATPGWQKWSRALSDAGLAALKATEAEDFKALVAANGQLVEACEGCHKEF
                     KPELPSEGIAHAHAH"
     gene            1957..2400
                     /locus_tag="AC16CY_09400"
     CDS             1957..2400
                     /locus_tag="AC16CY_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3354044.1"
                     /translation="MNPDVMTVADEMTTGEVARYLTEHEISGAPVVDSHGHLIGVVSM
                     TDIGRHMAEPSELESTRGSEFYRDPADEVTLEDFGQRYVEESATTVREVMTPVIHQVP
                     ATATVAEAARVMIREHIHRLVVTQGREPVGIITSMDLLRVVAELT"
     gene            complement(2467..3408)
                     /locus_tag="AC16CY_09405"
     CDS             complement(2467..3408)
                     /locus_tag="AC16CY_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR3354045.1"
                     /translation="MAMTLEYNATIVGRADLTDALSIFLIRPDRLPAKRPWFASGQYC
                     VLGLNNTDDPALGSVRRPMSIASPPEDDGPIEFYIRRVSKPESKNPLTHLLWRLGRGD
                     RIYMRTDAAGVFTIADTTGREDRRMRILVASGTGLAPFVSMVRSEVRRNPRADLSGWV
                     LLHGASRAAELGYRQELLGLSSTNRLRYWGTVSRPTETPEWVGDVGRVETFFEPARLR
                     DLERRLNLPPDGLTPHGAVVYVCGLTGTITATMDRLIDRGFIPHALPIREALGVPSDA
                     RDSLFFELYDPAPLIDFTDPSVVQPLRARMRAALASL"
     gene            complement(3495..3971)
                     /locus_tag="AC16CY_09410"
     CDS             complement(3495..3971)
                     /locus_tag="AC16CY_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354046.1"
                     /translation="MRSLILAFCLALVSPLSIPPFSAAQRFVATEPLLALPDGEESWV
                     IHIEKRGGLVDNREHLVTIASSGQLVCGGQLRSCVDRVGTEALARVAAHISKLPPQGS
                     ELVSVCSDCTFTVLTVRHRQPSSEVIDRIFSWTVESTRASNIAQQLYEIVVREVAA"
     gene            4075..>4740
                     /locus_tag="AC16CY_09415"
     CDS             4075..>4740
                     /locus_tag="AC16CY_09415"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR3354047.1"
                     /translation="MGRVWYRLQVVMRRLLDGGAGDAELSEEIRAFVAHDADSKTRSG
                     MTPDEARRAALIELGGAEQVKEHVREARAGARWEGVFRDIRYALRGLGRSPAFSFSVI
                     GNLSLGLAAMIGAFALINGSLRRPSPGIHDPDRVVEIGILENRPIGSRAARLVSADYP
                     DVARVLEEGIPSLEGLASFTTSDVAVTLPQPRSLPAAFVSPNYFDVLGVRPEIGRTLA
                     PREE"
BASE COUNT          904 a         1472 c         1597 g          767 t
ORIGIN      
        1 ggagcgctat cgcaccccat cccgcccgta acgggaacta ttccgcagcg cccgggatta
       61 atccgatacc ccgtgacccc gcctccaccg ttgccgcacc aagactgagg agcgcgggtc
      121 gaatcacgcg gcacaggcgt tgctgcaacc tatggcatgc ccaggccgct tccggcaacc
      181 gcttcgatga actgcccgct ctgcgggcag tgggttgtgc ccacggtgga cagcacgtgg
      241 gtgctcgcct ggtacgactg cccgggctgc ggccacacgt ggtcggcgcg cattcgtaac
      301 ggtcaacccg acctgccgtt ggctgccgat gcctgcctgt actcattgcc ccacgaggag
      361 aggccgtgac tcggacaaac ccgacaccaa tcagcgaatc gcatccgggc ccggggtccg
      421 gcgagccccc ggtcgtctac tggcaccggc agcttccgcc gctcgaggcg gagatgatcg
      481 gcgagcatat ccttgaagcg accagcggcc gcgtgcaggg aacgctggcg caccgtgacg
      541 agctctggga tcaatgctac cggagcctca ttgcgaacac ggaagagagg tttgcacagg
      601 aggccgcccg cctgggcggc cattacgcgc acgtgctgga cgaatcgatc gacagccgcc
      661 gtgacgacgc ggcgggggag gcgtggctgc acgggcgctt cacgtacatg ttgtaccgac
      721 gggccacgcc ggacgcgcgg caccgggtca cggccggact gggaacggcc gtcgcgctcg
      781 tggcgggtat ggccctcatc ccgtcaatgg ccgtgcacgc acagaagcag catcctcccc
      841 tcgagacacc cgaggcggtc gaagcgtcga tgaaacactt ctatcgtgcg gccaacgtca
      901 tcatcgtcac gacgatggac ggcatggagc acgtctacca cttcacgaga gacctggtcg
      961 tgcacggtgg aaagaagccc ggcgtcgacg ccctggaagg gctgcgcgag gggacgatga
     1021 tcgtcgtcca tcgcaacgtg agcggaccgg agacgtcggt tgcggaaatc gatctcgttg
     1081 gcgacgaagg attgaagatc accgagggcg tggtgaccga catcaatcgg cgcaaacagg
     1141 agatcacgat ccagtacacc aacgggacga ccgagacgtt gaaattgacc gctcaggctg
     1201 ccgccgagaa cgatacgctc gcgagatcgg atagcacgag gatgcgcatc gtcatctact
     1261 acgcggacga agcggggcgc aaggtggcgc actacatcaa gggctactga gttcgtgcaa
     1321 accgggttgc ccataaggag cgcatcatga cacgtacgtc catgcacata ggtttcgtca
     1381 tgattgtcgg catgtggctg atcgctggtt gtggtgcacc cgcgccggtc gagcagcaac
     1441 cggcggctgc accgcctcct ccagcggcgc cagtcgtcat gtctccggtc agcatcaatg
     1501 cggagatggt ctcgatcgtg gaccacgccg gccacgagct gtggaacgtc gagcgccaag
     1561 gaagggcgcc gaaagccagg aaagactggg agaacctcgc ggaacacgca actcagctgg
     1621 cggcggcggg cgctctggtc agagtcgcgg gaacgggtgt caacgacgtc acctgggtcg
     1681 cgacgccagg atggcagaag tggtcgcggg cgctgtcgga cgccgggctc gcggcgctga
     1741 aagcgacgga agcagaggat ttcaaggccc tggtcgcagc caacgggcag ctggtcgagg
     1801 cgtgcgaagg ctgccacaag gagttcaagc cggagctccc gagcgaaggg atcgctcacg
     1861 cgcacgctca ctgatgatat ttgcgcttgg ggcggcgatt gaccgcgaca cggtaggagc
     1921 gatgccatga acaaagcgag atcggtgaag gacgtaatga accctgatgt catgaccgtc
     1981 gccgacgaga tgacgacggg cgaggtggct cgatacctga ccgagcatga gatctccgga
     2041 gcaccggttg tcgacagcca cggtcacctc attggcgtcg tgtccatgac cgacatcgga
     2101 cggcatatgg ctgaaccatc tgagctcgaa tcgacacgag gttcggagtt ctaccgggac
     2161 ccggctgatg aggtgacgct cgaggatttc gggcagcgct acgtcgagga gtccgcgacg
     2221 accgtgcgcg aggtcatgac ccctgtcatt caccaggtcc cggccactgc gacggtcgca
     2281 gaagcggcgc gggtgatgat tcgcgagcac attcaccggt tggtcgtcac gcagggcagg
     2341 gagccggtcg gcatcatcac cagcatggac ctgctgcggg tggtcgccga actgacctga
     2401 cagcggtcgc aggggatggc gtcacggggg acccgcgcga cgtggcgctc agggggcgcg
     2461 aaggacctac agcgacgcca gcgccgcgcg catgcgtgcg cgcaacggct gcacgacgga
     2521 gggatccgtg aagtcaatca ggggcgcggg atcgtagagc tcgaagaaca ggctgtctcg
     2581 tgcatcggag gggacaccaa gcgcctcccg gatcggaagc gcgtggggaa tgaagccgcg
     2641 atcgatgagc cgatccatgg tggccgtgat ggtgccggtc aaaccgcaga cgtagaccac
     2701 ggcgccgtgc ggcgtcagtc catcaggcgg cagattcaga cgccgctcga gatcacgaag
     2761 tcgggcgggt tcgaagaacg tttcgactcg ccccacgtcg cccacccact ccggcgtctc
     2821 cgtcggacgg ctgaccgtac cccagtatcg aaggcgattg gtcgacgaca gtccaaggag
     2881 ctcctgacga tagccgagct cggctgcgcg ggacgcacca tgaagaagca cccagcccga
     2941 gagatccgcg cgcgggttgc gacggacttc gctgcggacc atgctgacga agggcgccag
     3001 gccggtaccc gaggcgacca ggatgcgcat tcgtcgatcc tcgcggccag tcgtatcggc
     3061 gatcgtgaag acgccggccg catcggtccg catgtagatc ctgtcgcccc gcccgagcct
     3121 ccacaacagg tgcgtgagcg ggttcttcga ttccggcttc gagacgcgac ggatgtagaa
     3181 ttcgatcggg ccgtcgtctt cgggcgggga cgcgatcgac atcggtcggc gaaccgagcc
     3241 tagcgccggg tcgtccgtgt tgttgagtcc gagcacgcag tactggcccg acgcgaacca
     3301 ggggcgcttc gccggcaacc ggtcgggccg gatcagaaag atcgacagcg cgtccgtcag
     3361 atcggctcgc ccgacgattg tcgcgttgta ctccagcgtc atggccatcg ctcatcttga
     3421 ttcacgcacg gactgacgga tcgctccacg cccgcgttcg agccgcgcgc acattgtgga
     3481 gagatggtca gccgctacgc cgcaacttcg cggacgacga tctcgtaaag ctgctgcgcg
     3541 atgtttgacg cgcgtgtgga ttcgacggtc caggagaaga tccgatcgat gacctcgctc
     3601 gagggctgac gatgtcggac tgtcagaacc gtaaatgtgc agtcgctgca gacgctcacg
     3661 agttcgctgc cttgcggtgg gagtttcgag atgtgtgcag cgactctcgc cagggcctcg
     3721 gttcccaccc ggtcaacaca cgatcgcagc tgtccaccac acaccaattg tcccgacgat
     3781 gcgatagtca ccaggtgctc gcgattgtcc acgagcccgc cacgcttttc gatatggatc
     3841 acccatgatt cctcgccgtc tggcagcgcg aggagcggct cggttgcgac gaatcgttga
     3901 gcggccgaga aaggagggat tgacagaggc gacacaaggg ccaggcagaa ggccagaatc
     3961 agggatcgca tttgtcgaaa gtattagacc acgatttgct ggattccagt gctcctgttg
     4021 acacacgata ggtctgtgtg ctctcctgac gtcatccgat aggagccgcc acacatggga
     4081 cgcgtctggt atcgcctgca ggtcgtgatg cgccggctgt tggacggcgg cgcgggagac
     4141 gcagagctca gtgaggagat ccgcgcgttc gtcgcgcacg acgccgactc gaagacccgg
     4201 tcgggcatga ccccggatga ggcacggcgc gcggcactga tcgagctcgg gggcgccgaa
     4261 caggtcaagg agcatgtccg ggaagcccgc gctggagcgc gctgggaagg cgtcttccgg
     4321 gacatccggt acgccctgcg cggtctcggt cggtcacctg cgttctcgtt ctcggtgatc
     4381 gggaatctga gcctgggcct ggcagcgatg atcggggcct tcgcgctgat caacggctcg
     4441 ctgcgccgtc cgtctccggg catccatgat ccggatcgcg tcgtggagat cgggattctg
     4501 gaaaaccgcc ccatcggatc gcgcgccgcg cggctcgtat cggcggacta cccggacgtc
     4561 gctcgcgtgc tcgaagaggg gatccccagc ctggagggtc tggccagctt caccacatcc
     4621 gacgtggcgg tgaccctgcc gcaaccgcgt tccctcccgg ccgccttcgt ctcgcccaac
     4681 tatttcgacg tgctcggagt ccggccagag atcggacgca cgttggcgcc ccgggaggag
//
LOCUS       DBNGSH010000410         1586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1224943, whole genome shotgun sequence.
ACCESSION   DBNGSH010000410 DBNGSH010000000
VERSION     DBNGSH010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1224943"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="AC16CY_09420"
     CDS             <1..238
                     /locus_tag="AC16CY_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014037.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR3354048.1"
                     /translation="TRALHPFTRCMTCNGRLAPVTRGRVAGLVPPRVYRRYRAFTRCR
                     NCQRIYWRGTHYVRLRRMVARLRSPRGPVRSNVS"
     gene            235..1029
                     /locus_tag="AC16CY_09425"
     CDS             235..1029
                     /locus_tag="AC16CY_09425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3354049.1"
                     /translation="MRCTAALVSVLVLTTACSVQSRRVEFPPGDIVDLSHTYDAQTIY
                     WPTAEGFQLRRDAEGVTPSGYYYASNSFFTSEHGGTHIDAPVHFAEGRQTVDKIPFER
                     LIGPAVVIDVTEQSGRDGDYQVTTQDLQRFEEQHGQIPPGAIVLIRTGFASRWPSAER
                     YLGTTERGAEGVRKLHFPGLHPDAARWIVANRPIGAIGIDTASIDYGQSTLFESHRAL
                     YAEDIPAFENLASLERLPATGALVIALPMKVGGGTGAPLRALAVLP"
     gene            1137..>1586
                     /locus_tag="AC16CY_09430"
     CDS             1137..>1586
                     /locus_tag="AC16CY_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3354050.1"
                     /translation="MAVANPYAEDLGDRPPLQALSETPDRIRQLVEHWSADRFERSYA
                     PGKWSARLVLVHLAQMELALGARARFALAERGYVAQPLSQDLWMSIDGAADARTALDA
                     YVALRRMNLLMWRTLTPEQLRRPFSHPEYGDLTVDWIMAQMAGHDIHH"
BASE COUNT          247 a          542 c          529 g          268 t
ORIGIN      
        1 gacgcgtgcg ctgcatccgt tcacgcgttg catgacctgc aacggccgcc tcgcgccggt
       61 gacgcgtggg cgtgtggccg gtctggtgcc tccgcgcgtg taccgcaggt atcgcgcgtt
      121 cacccgctgc cggaactgcc agcgcatcta ctggcgcgga acgcactacg tacgcctgcg
      181 gcggatggtg gcgcgtctgc gctcgccacg cgggccggtg aggagcaacg tgtcatgaga
      241 tgcaccgctg cgctggtctc ggttctggtg ctgacaaccg cctgttcggt gcagtcccgc
      301 agagtcgagt ttccgccggg cgacatcgtg gatctgtcgc acacctacga cgcgcagacg
      361 atctactggc ccacggccga aggctttcag cttcgacggg atgccgaggg cgtgacgccg
      421 tcggggtact actacgcctc gaacagcttc ttcacgtccg agcacggcgg cacgcacatc
      481 gacgcgccgg tccatttcgc agagggacgg cagacggtgg acaagattcc gttcgagcgc
      541 ctgattggac cggcggtggt gatcgacgtg acggagcaga gcggacgcga cggggattat
      601 caggtgacga cccaggatct ccagcgcttc gaggagcagc acggtcagat tccccctggc
      661 gcgatcgttc tcatcagaac cggcttcgcg agccggtggc ccagcgcgga gcggtatctc
      721 ggcacgaccg agcgcggcgc cgagggcgtg cgcaagctgc atttcccggg gctgcacccc
      781 gacgccgcac gctggatcgt cgccaatcgc cccatcggcg cgatcggcat cgacaccgcc
      841 agcatcgact acggacaatc gacgctcttc gagtcgcacc gcgccctcta cgcagaggac
      901 atcccggcct tcgagaacct cgcctcgctc gagcgtctgc ctgcgacggg ggcactggtg
      961 atcgcgctgc cgatgaaagt tggaggcggc acgggcgcac cgctgcgcgc gctggcggtg
     1021 ctcccgtgac cctggtctcc tgactgccac cgcgaggctg gccagctttg tccaccgcat
     1081 tgcgccgtgc gaaggcggac tcttgacgcg gcccggtttt cgatccctga tcgggtatgg
     1141 ccgttgcgaa cccgtacgcc gaggacctgg gcgatcggcc gcctcttcag gcgctgtcgg
     1201 aaaccccgga ccgcatccgc caactggtcg agcactggag cgccgatcgc ttcgagcgca
     1261 gctacgcgcc cggaaagtgg agtgcgcggc tggtgctcgt ccatctcgcc cagatggaac
     1321 ttgccttagg tgcccgcgcc cggttcgcac tcgctgaacg cgggtacgtc gcgcagccgc
     1381 tctcgcagga cctctggatg tccattgacg gcgccgccga tgcgagaacg gcgctcgacg
     1441 cgtacgtcgc gctgcgccgc atgaatctgc tgatgtggcg cacgttgacg cccgagcagc
     1501 tgcgccgccc tttctcgcac ccggagtatg gcgacctgac cgtcgactgg atcatggcgc
     1561 agatggcggg acacgacatt caccac
//
LOCUS       DBNGSH010000411         3078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4113081, whole genome shotgun sequence.
ACCESSION   DBNGSH010000411 DBNGSH010000000
VERSION     DBNGSH010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4113081"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="AC16CY_09435"
     CDS             complement(<1..778)
                     /locus_tag="AC16CY_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354051.1"
                     /translation="MIEGLLQDLKHGSRMLVKNPGFAFIAVLSIAIGVGANAAMFSFA
                     DTLVLRPLTVPRPDEIVTVSAVVPRSGFAPPTSGALSYPDYVDIRDQSRSFASLVAYQ
                     LIVASVADRRDEPARRTFGMAVSGNLFDALGVQPALGRAFGAEEDRVGGQNPVVVLDH
                     DAWQNEFAGDPGVLGRTIRIGGAEMTVIGVMPPGFSGPDQWVLPGYYLPIAMLPRLQG
                     IPADALTARGLRNFIVKGRLHEGVSVAQANEEVSLIGAVLQ"
     gene            complement(933..>3078)
                     /locus_tag="AC16CY_09440"
     CDS             complement(933..>3078)
                     /locus_tag="AC16CY_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354052.1"
                     /translation="NLTGIDVPIRVSAIRVTANLPALWGFTPILGRGFRPEEGQAGAT
                     RATLLSHGFWQRQFSSNAGILGQSLLLDGVPHTVIGVLPPVAGTGLFKDADVFVPLVL
                     DPLRTGRDERNVIVTGRLKPGVTRAQASADLDAIARRLQVEHPETNQRIGASVLPIIE
                     ASGFNVRLLLSLLGTIALLVLIVACANVANVMVAQSLGRRHELAIRTALGASRADRIR
                     QLMTESTLLSLTAGGVGLLLAAWGVDALRWLGGDSFGFAEIRISGRVLVAGFVTALVA
                     PLGFGLLPAIGAAAPDPQELKDGAREVSASSRTRRVRSVIIGLQAGAAMVLMVQIGLL
                     VRTTWTLSEIRPGFDPAQVLTFHVGLAGSKYEEPGTIDRFANEVMSRLRALPGIASVG
                     IVDRLPIVDSEPMVRLTVEGDAPARLEARPLVARAAIAGDYFTTLRIPIRRGRAFSEA
                     ESANTSPVAVINEEAARRYWPGRDPLGSRVALDVPGSDPTTRSDSTGLTPAWLTVVGV
                     VGNTRNSDVDQGSLPLVYVSATRQPSPQFGVVVKTSVADPLQLVPAIRAQVAGIDRDQ
                     PIHDVATMSQVLFDDLGSTYVLAAMLTVIGLVALLLSAAGIYGVVSHAVAQRRREIGV
                     RLALGARQGAIVRMVVGHGTRPVLAGGLIGLLAAVAIAYWSATALSEIEARNPLTYIV
                     VAVMIAIVSLAASYLPARRAASIDPVVALRQE"
BASE COUNT          563 a         1023 c         1008 g          484 t
ORIGIN      
        1 gctgcagcac ggcgccgatg agcgagactt cttcgttggc ctgggccacg gagacgccct
       61 cgtgcaggcg tcccttgacg atgaaattgc ggagcccccg cgcggtcagc gcgtcggccg
      121 ggatgccttg gagccggggg agcatcgcaa tcgggaggta gtaaccgggc aggacccact
      181 ggtccgggcc gctgaagccc ggcggcatga cgccgatgac ggtcatctcg gccccgccga
      241 ttctgatggt gcgcccgaga acccccgggt cgcccgcaaa ctcgttctgc cacgcgtcgt
      301 gatcgagtac gacgacgggg ttctgtccgc cgacgcggtc ttcctcggcg ccgaacgctc
      361 gaccgagagc cggctgtacc ccgagcgcgt cgaacaggtt gccgctcacg gccatgccga
      421 atgtccgccg cgccggttca tcccgtcgat ccgcgacgct ggcgacgatg agctgatagg
      481 caacgaggct cgcgaagctt ctcgactggt cgcggatgtc cacgtaatcg gggtaggaca
      541 gcgccccgga ggtcggcggc gcgaagcccg agcgcggtac cacggccgag accgtcacga
      601 tctcgtcggg gcgcgggacg gtcagcggcc gaagcaccag cgtgtcggcg aagctgaaca
      661 tcgccgcgtt ggcgccgact ccgatggcga tcgacaggac tgcaatgaag gcgaagccag
      721 ggttcttcac gagcatccgc gagccgtgct tcaggtcctg gagtaatcct tcaatcattg
      781 ttgacttacc gatctttgat tgtcgattcg ttgtcgattg ttgattcttg attggcgact
      841 catttgaacg attgaactca atcaacaatc aacaattgcc aatcaacaac gaatcaacaa
      901 tcaacaatcc caaatcccca atcaagaatg ccctactcct gtcgcaacgc caccaccgga
      961 tcgatcgatg ccgcacgcct ggccggcaga tagctcgccg cgagcgacac gatcgcgatc
     1021 atcactgcca cgacgatgta cgtgagagga ttgcgcgcct cgatttccga cagtgccgtt
     1081 gccgaccaat atgcaatcgc gacggccgcg agaagtccaa tgaggccgcc cgcgaggacc
     1141 ggcctcgtgc cgtgcccgac gaccatccgt acgatcgcgc cctggcgggc gccgagcgcc
     1201 agccgtacgc cgatctcccg ccgacgctgc gcgacggcgt gggacaccac accgtagatg
     1261 ccggccgccg agaggaggag cgcgaccagc ccgatcacgg tgagcatcgc ggccagcacg
     1321 taggtgctgc ccaggtcgtc gaagagcacc tgcgacatcg tggcgacgtc gtgaatcggc
     1381 tgatcgcgat cgataccagc gacctgagca cgtatcgccg ggacgagctg cagcgggtcg
     1441 gcgacagagg tcttgacgac gacgccgaac tgcgggctcg gttgccgggt cgccgagaca
     1501 tagaccagcg gtagcgaccc ctgatcgacg tcggagtttc gcgtattgcc gacgacgcca
     1561 acgaccgtca gccatgccgg ggtcagaccc gtcgagtcgc tccgtgtcgt ggggtctgac
     1621 cccggtacgt cgagggcgac gcgcgagccg agcggatccc ggcccggcca gtaccgcctg
     1681 gccgcctctt cgttgatgac ggcgacaggg gaggtgttcg ccgattcggc ctccgaaaac
     1741 gcgcgcccgc gccggatcgg aatccgcagc gtcgtgaagt aatcgcccgc gatggctgcg
     1801 cgcgcgacca ggggtctcgc ttcgagccgc gccggagcgt cgccttcgac cgtgagccgg
     1861 accatgggtt cgctgtcgac gatcggcagc cgatcgacga tgccgaccga ggcgatgcct
     1921 ggaagggcgc ggaggcgtga catgacctcg ttggcgaagc gatcgatggt tcccggttcc
     1981 tcgtacttcg acccggccag cccgacatgg aaggtcaaca cctgcgccgg atcaaatccc
     2041 ggacggattt cgctgagcgt ccacgtcgtt ctcacgagca gcccgatctg gaccatcaac
     2101 accattgcgg cgcctgcctg cagaccgatg atgacgcttc ggacgcggcg agtccggctc
     2161 gaggcactca cctcgcgagc accgtctttc agttcctgtg gatcgggagc cgcggcgcca
     2221 atggcgggga gcaggccgaa gcccagcggg gcgacgagcg ccgtcacgaa ccccgcgacg
     2281 agcacacgac cgctgatccg aatctccgcg aatccgaacg agtcgccgcc cagccatcgc
     2341 agggcgtcga caccccatgc ggccagcagg aggccgacgc cgccggctgt cagcgagagc
     2401 agcgtgcttt ccgtcatgag ctgacggatc cggtcggcac ggctggcgcc gagcgccgtg
     2461 cggatcgcga gctcgtgccg gcggcccagc gactgggcca ccatgacgtt cgcgacgttt
     2521 gcgcatgcca cgatcagcac gagcagcgcg atcgttccaa gaagtgacag gaggagacgc
     2581 acgttgaagc ccgaggcttc gatgatcggc aacaccgacg cgccgattcg ctggttcgtc
     2641 tccgggtgct cgacctgcag acggcgtgcg atggcgtcga ggtcggctga agcctgcgcg
     2701 cgagtcacac ccggtttgag tcttcccgtc acgatcacgt tgcgttcgtc acgcccggtg
     2761 cgcagcggat ccagtaccag cggcacgaac acgtcggcgt ccttgaagag tccggtgccc
     2821 gcgaccgggg gaagaacgcc gatcacggtg tgaggcacgc cgtcgagcag cagactctgc
     2881 ccgagaatgc cggcgttcga cgagaactgg cgttgccaga acccatggga cagcagcgtg
     2941 gcccgtgtcg cccccgcctg tccctcttcg ggccggaatc cacgtccgag aatcggagtg
     3001 aagccccaga gcgctggcag gttcgccgtc accctgatgg ccgacacccg gattggtaca
     3061 tcaatgccgg tcaggttt
//
LOCUS       DBNGSH010000412         2676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2754448, whole genome shotgun sequence.
ACCESSION   DBNGSH010000412 DBNGSH010000000
VERSION     DBNGSH010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2754448"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16CY_09445"
     CDS             <1..615
                     /locus_tag="AC16CY_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003138995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR3354053.1"
                     /translation="HGVTGRDASFVQSIDGPAIGPFLYLGAKHMVTGYDHLLFLVGVI
                     FFLYRIKDVVLYVSLFTVGHSLTLLGGVLGDIRANAYLVDAVIGLSVVYKALENMGGF
                     DRLGLRIDTRAAVMIFGLFHGFGLATKLQEFTVSQNGLVTNIVSFNVGVEIGQGLALT
                     AILIGISIWRTRPGFLRHAFATNTALMVGGFILMGYQLTGYFQS"
     gene            612..1226
                     /locus_tag="AC16CY_09450"
     CDS             612..1226
                     /locus_tag="AC16CY_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354054.1"
                     /translation="MSTAPQVSPPAPSGKALVKATLLALVVAGIVLVTAVLPAEYGID
                     PLGTGRVLGLNNLFAAEDAPAGPRTIVPDSASPVHPQLNEYRVDSRVLKIGPKEGIEF
                     KYDLDKGATMLYAWKADAFVDFDFHTEPEGKPPEASDSFEKGQAAQKRGAYTAPYDGI
                     HGWYWENPTDREVTVTLSSTGFYQSARLFLPQRPVQFIEIPATP"
     gene            1265..>2676
                     /locus_tag="AC16CY_09455"
     CDS             1265..>2676
                     /locus_tag="AC16CY_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR3354055.1"
                     /translation="MRKWLILTHRYIGIALGLLFVMWFATGIAMLYARGMPRLTADLR
                     LERLEPVDVSSVRLSAAEAAGRAEISDAPGGVTLLTVLGRPAYRFNIRGESVTVFADT
                     GDLLADVDQNVARAIGAKFAGVPEDRVHHVERLTRPDQWTLTIRRGAMHRFTVDDESR
                     TELYVSERDAEVAAVTTRGSRALAWVGAIPHFFYYAPLRLNQALWRQVVLWTSGIGIF
                     LAFLGIVLGIVQLRKARPFTLSRAVPYTGWMKWHHVTGLVFGVFTLTFVFSGWLSMEP
                     WGWATSQGRFGGGLREAFTSAPADLAAFPRLTAAGWPDIAGTRAIKEIEYVRIQDEPF
                     FVVRREPDANAGTEIRGHEPYFAARSSDAAQLVVNARTMEVHDRDFSADELLHRVRGA
                     LPGTEVSRSEMLATYDSYYYSREGLAPLPVLRVKLADPESTWLYIDPRMSRLVGRVHR
                     LDRVERWLYNGLHSLDFSFW"
BASE COUNT          428 a          877 c          896 g          475 t
ORIGIN      
        1 cacggggtga cggggcgcga cgccagcttc gtgcaatcga tcgacggtcc cgcgatcggg
       61 ccgttcctgt acctcggcgc caagcacatg gtcaccggct acgaccacct gttgttcctc
      121 gtgggcgtca tcttcttcct ctaccgcatc aaggatgtcg tgctctacgt gagcctgttc
      181 accgtcgggc acagcctcac gctgctcggc ggcgtgctcg gggacatccg tgcgaacgcg
      241 tatctggttg acgccgtcat cgggctgtcg gtggtctaca aggcgttaga gaacatgggg
      301 gggttcgatc gcctggggct gcgaatcgat acgcgggccg cggtgatgat cttcggcctg
      361 ttccacgggt tcggcctcgc caccaagctc caggagttca ccgtgtcgca gaacggcctg
      421 gtgaccaaca tcgtcagctt caacgtcggc gtggagatcg ggcaggggct cgcgctcacc
      481 gcgatcctca tcggcatcag catctggcgc acgcggcccg gcttcctgcg gcacgccttt
      541 gccaccaaca ccgcgctgat ggtgggtggc ttcatcctga tgggatacca attgacgggg
      601 tacttccagt catgagcacc gcacctcagg tctcgccgcc ggccccgtcg ggcaaggcgc
      661 tcgtcaaagc aactcttctc gccctggtcg ttgccggcat cgtgctggtc acggccgtgc
      721 tgcccgcgga atacgggatc gatcccctcg gcacggggcg cgtgctcggc ctgaacaacc
      781 tgttcgccgc cgaagacgcg cccgcgggac cgaggaccat cgtgccggac tcggcaagtc
      841 cggtgcaccc gcagctgaac gagtaccgcg tggattcacg cgtgctcaag atcggcccga
      901 aggagggcat cgagttcaag tacgacctcg acaagggcgc gacgatgctg tacgcgtgga
      961 aagcggacgc gttcgtcgat ttcgatttcc acaccgagcc cgaaggcaag ccgccggaag
     1021 cgtccgacag cttcgagaag gggcaggcag cgcagaagcg tggcgcctac acggcgccgt
     1081 acgacggcat ccacggctgg tactgggaaa acccgactga tcgtgaagtg acggtgaccc
     1141 tgtcgtcgac cggtttttat cagtcggcgc ggctgtttct cccgcaaagg cccgtacaat
     1201 tcatcgagat cccggccaca ccctgactta ggggcgcgcc ggactgtgaa agggtctgac
     1261 cccgatgcgt aagtggctca tcctgaccca ccgctacatc ggcatcgcgc tcggtctgct
     1321 cttcgtcatg tggttcgcca ccggcatcgc gatgctgtac gcgagaggta tgccccgcct
     1381 gacggcggac ctccggctcg agcgcctcga gccggtcgat gtgtcgagcg tgcgtctgtc
     1441 ggcggcggaa gccgccggac gcgcggagat ctccgatgct ccgggcggcg tgacgctgct
     1501 caccgtgctg ggcaggccgg cctaccgctt caacatccgc ggggagtcgg tgacggtctt
     1561 cgccgatacc ggtgacctgc tggcggacgt ggaccagaac gtcgcccgcg cgattggcgc
     1621 gaagttcgcc ggcgttcccg aagaccgcgt ccaccacgtg gagaggctga cccgccccga
     1681 ccagtggacg ctgacgatcc gccgcggcgc gatgcacagg ttcacggtgg acgacgagtc
     1741 gcgcaccgag ctgtacgtgt ccgagaggga cgcggaagtc gccgccgtca cgacgcgagg
     1801 cagccgtgcg ctggcgtggg tgggcgcaat cccgcacttc ttctattacg cgccgctgcg
     1861 gctcaatcag gcgctctggc gtcaggtcgt gctgtggacc tcgggcatcg gcatcttcct
     1921 cgcctttctc ggtatcgtcc tgggcatcgt ccagctgcga aaggcaaggc cgttcacgct
     1981 gtcgcgtgcg gtgccgtaca ccggctggat gaagtggcac cacgtgaccg ggctcgtctt
     2041 cggagtcttc acgctgacgt tcgtcttcag cggatggctg tcgatggagc cgtggggctg
     2101 ggcgacgagc cagggacggt tcggtggcgg ccttcgagag gcgttcacga gcgctccggc
     2161 cgatctggcg gcgttcccgc ggctgaccgc cgccggctgg ccggacatcg ccggcacgcg
     2221 cgcgatcaag gaaatcgagt acgtgcgcat ccaggacgag ccgttcttcg tcgtacggcg
     2281 cgaacccgac gccaacgcgg gcactgaaat ccgggggcac gaaccgtact tcgccgcccg
     2341 gtcgagcgac gccgcgcagc tcgtcgtgaa cgcgcgaacg atggaggtgc acgaccgcga
     2401 cttctcggct gacgaactgc tccaccgcgt caggggcgcg ctgcccggaa ccgaggtgtc
     2461 ccgttcagag atgcttgcaa cctacgattc gtactactac tcccgcgagg ggctggcgcc
     2521 actgccggtg ttgagggtga agctggcgga cccggagagc acgtggttgt acatcgatcc
     2581 gcgcatgagc cggctggtgg gccgcgtgca tcgcctcgac cgcgtcgagc gatggctcta
     2641 caacggcctg cacagcctgg acttctcgtt ctggta
//
LOCUS       DBNGSH010000413         3471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1056796, whole genome shotgun sequence.
ACCESSION   DBNGSH010000413 DBNGSH010000000
VERSION     DBNGSH010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1056796"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC16CY_09460"
     CDS             complement(<1..362)
                     /locus_tag="AC16CY_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354056.1"
                     /translation="MRAHVIALTCVVLVVIAAPRVSAQESLDAARELYSSAEYDSALA
                     MLDGLLRNGSTGEDRHSIEMYRVLCLMAMRRDGEARGAIETLVVHNPLYRPASDVPPR
                     IRNVYDETRRRVLPSAVQA"
     gene            complement(368..2566)
                     /locus_tag="AC16CY_09465"
     CDS             complement(368..2566)
                     /locus_tag="AC16CY_09465"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3354057.1"
                     /translation="MESAVARGSSSAQVTFEDGLGQRLTSVSVDGEPLEVLALRDELT
                     ANSAFELALRDRVNRLADFESEHFARVRAVERIGKGSRLAIISDRVQGVRLSEILSAA
                     ESRLLPVETDAALSLLRQLVHAISVLHDKAPELCHGAIGPERLIVTPAGRLMVVEYVL
                     GSAIEQLRLSNKHYWQRLRVPLPRTLGMPHFDRRSDVTQIGATALALVIGRPLAEDEF
                     PGRVGDMTGGALAISADGGIEPLPLPLRVWLQRALQIDSRTPFGSALEAWADLDRVLH
                     YSDPIAEVESLKSFLGRYEADASSDDVATLVAPPFAAPPAAAPPARPVDAIPPRPPVP
                     PPVTVSTQASAPPAPKMTAVPPKPAVPALAPVTAPAAAASAVSPMPSFWDRLPSPAID
                     DSAGPDDLEVRKERNAVPVIASTRLRMVAAAVLLIALTAGATLATRRYLLPSVQADDA
                     LGTLIVQTNPSGATVEIGGQQHGVTPLSLSLPPGRHTLKLTSEGNVRTMPVTIAAGKE
                     VSQFIELPRASSAFGELQVRTEPAGARVTVDGHAYGTTPVTVEGLSPGTHTVVLENEL
                     RSMTQEVKIDANTTASLVVPLTAPANAPLSGWISVDAPAELRIYEGQRLIGTSQTDRI
                     MMSVGRHTLQVANEALGYRETTVVNVAPGKVSSVQPPWPSGLVSLNAAPWAQVWIDGQ
                     SVGETPLSNIRVPIGSHEVVFRHPELGEKLVRTVVTVGTPTKVSADLRQR"
     gene            complement(2789..>3471)
                     /locus_tag="AC16CY_09470"
     CDS             complement(2789..>3471)
                     /locus_tag="AC16CY_09470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3354058.1"
                     /translation="LEKTAQEIRSESGVQVLAVPADITKSDQIDACAQAAKEAFGVVH
                     IVVNNAGGPMRRMDRQILWPDSDWIADLDNKILGALRVTRAFLPLLPGDGTGRIINIS
                     GTASTLVWAPALTHGLNNAAMNQATGYLATDLAGQRVTVNTIVPGLVGTEGREAWAEN
                     MAKQQNVTKEEFVSSFCKRMGILAGRWATMDEVADTVLFVASDRGRYYNGAKIILDGG
                     LNVNLRPA"
BASE COUNT          560 a         1169 c         1172 g          570 t
ORIGIN      
        1 gcctggacgg ctgatggcag gacgcgccgg cgcgtctcgt cgtacacgtt gcggatccgc
       61 ggaggcacgt cgctcgccgg gcggtacaac ggattgtgaa cgaccagtgt ctcgatcgcg
      121 ccccgcgcct cgccgtcgcg ccgcatcgcc atcagacaca gcacgcgata catctcgatg
      181 gagtggcggt cctctcccgt gctgccgttt ctcaacaggc cgtcgagcat ggccaacgcc
      241 gaatcgtatt ccgcggagga atacagctcg cgcgcggcgt cgagcgactc ctgagcgctg
      301 acgcggggcg cggcaatgac gacgaggaca acgcacgtga gagcgatgac gtgggcacgc
      361 atggccatta cctctgcctc agatccgcgc tcaccttggt cggcgtccca acggtcacga
      421 ccgtacggac cagcttttcg ccgagctcag ggtgacggaa caccacctcg tgcgatccaa
      481 tcggcaccct gatgttgctg agcggggtct cgccgacaga ctggccgtcg atccagacct
      541 gggcccaggg ggccgcgttg agcgacacca gcccgctcgg ccagggcggc tgcacggacg
      601 acacttttcc gggcgcgacg ttgaccaccg tcgtttcccg gtagccgagc gcctcgtttg
      661 caacctgaag cgtatggcgg ccgaccgaca tcatgatgcg gtcggtctga ctcgtgccga
      721 tgagccgctg gccttcgtag atcctcaact cggccggcgc gtccacggag atccatcccg
      781 agagcggcgc gttggccggt gcggtcagcg gcacgaccag cgacgccgtc gtgttcgcgt
      841 cgattttcac ttcctgcgtc atcgagcgga gctcgttttc gagcaccacg gtatgggtgc
      901 ccggcgacag cccctccacc gtgaccggcg tcgtgccata ggcgtggccg tccacggtca
      961 cgcgcgcgcc ggccggctcg gtacgcacct ggagctcgcc gaacgcggac gaggctcgcg
     1021 gaagctcgat gaactgcgac acctctttgc cggcggcgat cgtcaccggc atcgtgcgga
     1081 cgtttccttc gctcgtcagc ttcagcgtgt ggcgacccgg tggaaggctg aggctgaggg
     1141 gcgtgacccc gtgctgctgc cccccgatct cgacggtcgc cccgctggga ttggtctgca
     1201 cgatcagcgt gcccagcgcg tcgtctgcct ggaccgacgg caacaggtag cggcgcgtcg
     1261 ccagcgtcgc gcccgccgtc agcgcaatca ggagaacggc tgcagccacc attcgaagtc
     1321 gagtcgatgc aatgaccggc acggcgttcc tctccttcct tacctccaga tcgtccggac
     1381 cggctgaatc gtcgatcgcc ggtgacggca gtcgatccca gaacgagggc atcggagaca
     1441 cagcgctcgc ggcagcggcc ggcgcggtca ccggcgccag agccggcacc gcaggcttgg
     1501 gcggaacggc cgtcatcttt ggtgccgggg gcgcactcgc ctgtgttgac accgtcacgg
     1561 gcggcggcac cggcggcctg gggggtatgg cgtccacggg ccgagcaggc ggtgccgcgg
     1621 ccggcggcgc cgcgaacggc ggcgcgacta acgtcgcgac gtcatcggac gaggcgtcag
     1681 cctcgtaccg cccgaggaac gacttgagcg actcgacctc ggcaatcgga tcgctgtaat
     1741 ggagaacgcg atccagatcg gcccaggcct cgagcgcgga gccgaacggg gtccgcgagt
     1801 cgatctgcag cgctcgctgg agccagacgc gcagcggcag cggcagcggc tcgatgccgc
     1861 cgtcggccga gatggccagc gcgccgccgg tcatatcgcc cacccggccg gggaactcgt
     1921 cctcggcgag cggccggccg atgacgagcg ccagtgcggt cgcgccgatt tgcgtgacgt
     1981 cgctccggcg atcgaaatgc ggcatcccga gcgtccgagg cagcggcacg cgcagcctct
     2041 gccagtagtg cttgttcgac aggcgaagct gctcgatggc cgatccgagc acgtactcga
     2101 ccaccatcaa ccgccccgcg ggcgtgacga tgaggcgttc gggcccgatc gcgccgtggc
     2161 acagttccgg ggccttgtcg tgcaggacgg agatggcatg aacgagctgc cgcagcaggc
     2221 tgagggcggc atccgtttcg accgggagaa ggcggctctc ggcggccgac aggatctcgg
     2281 acagccggac accctggacg cgatccgaga tgatcgccag cctggagccc ttgccgattc
     2341 tctcgacggc gcgcacgcgc gcgaagtgct cggactcgaa gtccgcgagc cggttgactc
     2401 gatcccgcag tgcgagctcg aacgctgaat tcgcggtcag ctcgtcgcgc agagcgagca
     2461 cctcgagcgg ttcgccgtcg acgctgacag acgtgaggcg ctggccgagg ccgtcctcga
     2521 acgtcacctg cgcggacgaa gaaccacgcg cgactgcaga ctccatgtga ctcttttcgg
     2581 cgtgaaccat gccatgcagt actggtgcgc gccggaacgg gacgcctggt gcaggaaatc
     2641 gattgaaata ccgtgagatt cgtgagtacg cgcctgaccc gctcggaggc gtgcctggcc
     2701 gggcagcgcc aagccgttac agaatcgtcg cgggactctc gattttgtga gtccccgtcg
     2761 aacggacgga tggcgcgccg acgcgcgttc aggccgggcg gaggttcaca ttcaaaccgc
     2821 cgtccaggat gatcttcgcc ccgttgtagt agcgtccccg gtcggaagcc acgaacaaca
     2881 cggtgtcggc gacttcgtcc atggtcgccc agcgtcccgc caggatcccc atgcgcttac
     2941 agaagctgga gacgaattcc tctttcgtga cgttctgctg cttggccatg ttctccgccc
     3001 aggcctctcg gccttcggtg ccgaccaggc ccggcacaat cgtattgacc gtgactcgct
     3061 ggccggccag gtcggtcgcg aggtagcccg tggcttgatt catggccgcg ttgttgagcc
     3121 cgtgcgtcag agcgggggcc cacacgagcg ttgacgccgt tccgctgatg ttgatgatgc
     3181 ggcccgtgcc atcgcccggc aagagcggaa ggaacgcgcg cgtgacgcga agggcgccaa
     3241 gaatcttgtt gtcgagatcg gcgatccagt cgctgtcggg ccagagaatc tgccgatcca
     3301 tccggcgcat cggaccgccg gcgttgttca ccacgatgtg cacgacgccg aacgcctcct
     3361 tggcggcttg cgcgcacgca tcgatctgat cggacttcgt gatgtcggcg ggcacggcga
     3421 gcacctgtac gccgctctcg cttctgatct cctgggccgt cttctcgagc a
//
LOCUS       DBNGSH010000414         2299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1225444, whole genome shotgun sequence.
ACCESSION   DBNGSH010000414 DBNGSH010000000
VERSION     DBNGSH010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1225444"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2299)
                     /locus_tag="AC16CY_09475"
     CDS             complement(<1..>2299)
                     /locus_tag="AC16CY_09475"
                     /inference="COORDINATES: protein motif:HMM:NF019259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3354059.1"
                     /translation="TSARALLDKYCVTCHSDRVKTANLSLQGLDLASVADHAEVWEKV
                     IRKLRAGVMPPPDTPRPSLAEYEGLRDFLEGEIDRAVGTKPNPGSVVLHRLNRTEYAN
                     AIRDLLDLRIDATTLLPPDDSANGFDNIAGSLTLSPTLLESYTTAAARVARMAVGYWK
                     SPAEATYLTSSDASQNHRLEGMPFGTRGGIVARHDFPADGEYKFSIQNFGIGSFIPGE
                     QLALIIDGERAHVWPYRGVGLAVGMTSDTDGTLEVTVPVRAGSRLVGATFLATNYRPS
                     LDIIRHYDRQSLENNSIPQLQYFPVIGFVRIQGPFNAQRPDDSASRRKVFTCRPTLAQ
                     ENAPASAKASARSRRSSPVTEASEGGCARQILTSLARRAYRRPPAAQDLAALMTFFDE
                     GRKDATFDDGIEYALRFLLASPQFLVRAEREPANVRPGQPYRITDLELASRLSFFLWS
                     SIPDEELITVASQGRLGQPGELDRQVRRMLGDPRSDALVTNFAQQLLYLRNLPATSPD
                     GIFYPNWDDELRQSLKRESELFFESIIREDRNVVDLLTADHTFVNERLARHYGIPGVY
                     GSRFRRVTLPPEMDYRRGLLGKGSFLAVTWTQNFRSSPVKRGVWVLENILGTPPPEPL
                     PNVPALEDTKSDSGKVLTLREQMTRHRASPVCAGCHKIMDPIGFALENFDADASWRTR
                     QGGEGGTPIDVKVKLYDGQDVDGPVGLRTALLRYSPQFVRMFVEKTMTYAVGRGLDYT
                     DMPTVRAIARDAAKDDNRFSAIVAGV"
BASE COUNT          355 a          764 c          800 g          380 t
ORIGIN      
        1 acgccggcca cgatcgcaga gaagcggttg tcgtccttcg cggcgtcccg cgcgatcgcg
       61 cggaccgtcg gcatgtcagt gtagtcgagc ccgcgcccga cggcgtaggt catcgtcttt
      121 tcgacgaaca tgcgcacgaa ctgcggcgag taccgcagga gcgccgtccg caggccgacc
      181 gggccgtcca cgtcctgccc gtcgtagagc ttcaccttca cgtcgatggg cgtgccgccc
      241 tcgccaccct gcctcgtgcg ccagctcgcg tcggcgtcga agttctcgag ggcaaacccg
      301 atcgggtcca tgatcttgtg gcagcccgcg cacacggggc tcgcgcgatg gcgcgtcatc
      361 tgctcccgca gcgtgaggac cttgccgcta tcgctcttgg tatcctcgag cgcggggacg
      421 ttgggcaagg gctccggcgg cggcgtaccg agaatgttct ccagcaccca cacaccgcgc
      481 ttgaccggcg agctgcggaa gttctgcgtc caggtgaccg ccaggaagct ccccttgccg
      541 agcaggccgc ggcgatagtc catctcgggc ggcagtgtca cgcgtcggaa ccgcgacccg
      601 tagacgcccg gtatgccgta atgccgtgcg aggcgctcgt tgacgaacgt gtggtcggcg
      661 gtcagcagat cgacgacgtt gcgatcctcg cggatgatgc tctcgaagaa cagctccgac
      721 tcccgcttca gactctgccg cagctcgtcg tcccagttcg ggtagaagat gccgtcgggc
      781 gacgtggccg gcaggttgcg caggtacagc agctgctgcg cgaagttcgt gacgagcgcg
      841 tcggagcggg gatcgccaag catccggcgc acctgtcgat cgagctcgcc cggctgaccg
      901 agccgcccct gcgaggcgac cgtgatcagc tcctcgtccg ggatgctgct ccagaggaag
      961 aacgacaatc gcgacgccag ctcgagatcg gtaatgcggt acggctgacc cggacgcacg
     1021 ttagcaggtt ctcgctcggc cctcacgagg aactgcgggc tcgccagcag gaagcgcaag
     1081 gcgtattcga tgccgtcgtc gaaggtcgcg tccttgcgcc cctcgtcgaa gaacgtcatc
     1141 agcgccgcga ggtcctgcgc cgccggcgga cgacggtagg cccggcgcgc cagcgaggtc
     1201 aggatctgtc tcgcacatcc gccttcgctc gcctcggtta ccggcgagct acggcgggac
     1261 ctcgccgaag ccttggcgga ggcgggggcg ttctcctgcg ccagcgtcgg acgacaggtg
     1321 aacaccttcc gccggctcgc cgagtcgtcc ggccgctgcg cgttgaacgg accctggatg
     1381 cggacgaatc cgatcaccgg gaagtactgg agctgcggaa tggagttgtt ctcgagcgac
     1441 tgacgatcgt agtggcgaat gatgtcgagg ctggggcggt agttggtcgc caggaaggtc
     1501 gcgccgacga gccgcgagcc tgcgcggacg ggcacggtga cctcgagcgt tccgtcggtg
     1561 tccgacgtca tccccaccgc gagcccgacg ccccgatacg gccagacgtg cgcgcgctcg
     1621 ccgtcgatga tgagcgccag ttgctcgccg ggaatgaagc tgccgatgcc gaagttctgg
     1681 atcgagaact tgtactcgcc gtcggcgggg aaatcgtgcc gcgcgacgat gccgccgcgc
     1741 gtgccgaagg gcatgccctc gaggcggtgg ttctgcgagg cgtcgctcga cgtgagatag
     1801 gtcgcctcgg ccggggactt ccagtagccg acggccatgc gcgcgacgcg cgcggccgcc
     1861 gtggtgtacg actcgagcag cgtcggcgac agcgtcagcg acccggcgat gttgtcgaag
     1921 ccgttggccg aatcgtccgg cggcagcagc gtcgtggcgt cgatgcgcaa gtcgagcaga
     1981 tcgcggatgg cgtttgcgta ctccgtccgg ttcagccggt ggagaaccac cgatcccggg
     2041 ttcggttttg tcccgacggc gcgatcgatc tcgccctcga ggaagtcccg cagcccttcg
     2101 tactcggcaa gcgagggtcg cggcgtgtcc ggcggcggca tcacgcccgc gcgcagcttg
     2161 cggatcacct tctcccacac ttcggcgtga tcggcgaccg aggccaggtc gagcccctgc
     2221 agcgagaggt tcgccgtctt cacgcggtcg ctgtggcagg tgacgcagta tttgtccaga
     2281 agcgcgcgcg ctgacgtcg
//
LOCUS       DBNGSH010000415         1638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3433391, whole genome shotgun sequence.
ACCESSION   DBNGSH010000415 DBNGSH010000000
VERSION     DBNGSH010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3433391"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="AC16CY_09480"
     CDS             <1..1059
                     /locus_tag="AC16CY_09480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3354060.1"
                     /translation="AFVQDLLKRHNVPGVSIAVIRDFKILSTFVYGVADVETGAPVTA
                     DTMFQAASISKPVAAMASLKAIQDGLFMLDQDVNTILTSWKLPGGEFTKERPVTPRTL
                     MSHISGTGDGFGFPGYAPGAPLPTIPQILDGTAPSNLRAVRLERAPWTGFEYSGGAVM
                     IEQLALMDAAGKPFDRLAREWVLDPIGMTNSTFEQPLPQARAGRAARAHDRNGARMGD
                     PWHVYPEQAAAGLWTTPTDLARFATEVQLALLGRSARVLSRSTAMEMVTPVGVGPFAV
                     GFEVAKQGEGWYFRHGGSNWGFQCDLTAHRAKGYGAVIMTNGDAGGRVIQDLRKVIEK
                     EYGWDSLDSPVPRRYGPA"
     gene            1223..1447
                     /locus_tag="AC16CY_09485"
     CDS             1223..1447
                     /locus_tag="AC16CY_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354061.1"
                     /translation="MNIWMEFKNAILPWLLAGGVVAFLIAEHRRTTAALLDANTRLAD
                     AVEKQGKALDGVRGLLASQGYTLPPLTEKR"
     gene            1444..>1638
                     /locus_tag="AC16CY_09490"
     CDS             1444..>1638
                     /locus_tag="AC16CY_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354062.1"
                     /translation="MIRGLRVPVHLSSRDSRLIFDLADIWAARTGNEVVLVSANDHVH
                     NRRSAHYDGQAVDFHSSDMDG"
BASE COUNT          277 a          531 c          560 g          270 t
ORIGIN      
        1 gcgttcgttc aggacctcct gaagcggcac aacgtccctg gcgtcagcat cgccgtgatc
       61 agggacttca agatcctgtc cacgttcgtc tacggcgtcg ccgacgtcga gacgggggct
      121 cccgtcaccg ccgacacgat gtttcaagcg gcgtcgatca gcaagccggt cgcggccatg
      181 gcgtcgctca aagcgattca ggacgggctg ttcatgctgg accaggacgt caacacgatc
      241 ctcacatcgt ggaagcttcc gggcggcgag ttcacgaagg agcggcccgt cacgccgcgg
      301 acgctcatga gccacatctc ggggaccggc gacggattcg ggtttccggg ttacgcaccc
      361 ggcgcgccgc tgcccaccat cccgcagatt ctcgacggga cggccccgtc gaacctgaga
      421 gccgtccggc tggagcgcgc cccgtggacc gggttcgagt actcgggcgg cgccgtgatg
      481 atcgaacagc tcgcgttgat ggacgcggcc ggaaaaccct tcgaccggct cgcgcgcgaa
      541 tgggtgctcg atcccatcgg gatgaccaac agcacgttcg agcagccgct gccgcaggca
      601 cgggctgggc gcgccgcgcg cgcgcacgac cggaacggcg cgaggatggg cgatccctgg
      661 cacgtctatc ccgagcaggc agccgccggg ctctggacca cgccgacgga tctggcgagg
      721 ttcgcgaccg aggtgcagct cgcgctgctc ggacgatccg ctcgcgtgct ttcgcggtcc
      781 acggcgatgg agatggtcac gccggtcggt gtggggccgt tcgcggtcgg cttcgaggtc
      841 gcgaaacagg gcgaggggtg gtacttcagg cacggcggaa gcaactgggg attccagtgc
      901 gacctgaccg ctcaccgcgc caaggggtac ggcgccgtca tcatgaccaa cggcgacgcc
      961 ggtggccgcg tgattcagga cctgcgcaag gtcatcgaga aggagtacgg ctgggacagc
     1021 ctcgactcgc ccgtcccgcg gaggtacggc ccggcctgaa agtacttctc tcttcccgcg
     1081 aaggcgcggt tttcgcagta ccagactgtt tggtgtccgt ttcatggact tgatacacca
     1141 aaatcgtcag tacggcaccg cgtcttcgtc agtcgcggga gacgctgtgt gcagcggcat
     1201 gcagcgtcgg ggggaggggc acgtgaatat ctggatggag ttcaagaacg cgatcctgcc
     1261 gtggctgctc gcgggcggcg tcgtggcatt cctcatcgcg gagcatcgcc ggacgacggc
     1321 cgcgctcctc gacgccaata cgcgtttggc cgacgcggtc gagaagcaag gcaaggcgct
     1381 cgacggcgtg cgcggacttc tcgcatcgca ggggtacacg ctgccccctt taacagaaaa
     1441 gcggtgattc gcggacttcg ggtgcccgtg cacctgtcgt cgcgcgattc acggctgatc
     1501 ttcgacctgg ccgacatctg ggcggcacgg accggcaacg aggtcgtgct cgtctcggcg
     1561 aacgaccacg tccacaaccg gcgttccgcc cactacgacg gccaggcggt ggacttccat
     1621 tcgtccgaca tggacgga
//
LOCUS       DBNGSH010000416         4009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3094575, whole genome shotgun sequence.
ACCESSION   DBNGSH010000416 DBNGSH010000000
VERSION     DBNGSH010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3094575"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="AC16CY_09495"
     CDS             complement(<1..269)
                     /locus_tag="AC16CY_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3354063.1"
                     /translation="MVNGHSPALGLYLHIPFCSSICNYCNFNRGLYDAALKEQYIAAL
                     LTEVHAKADGAAADTVYFGGGTPSLLEPREIGRLVEACRQSFALS"
     gene            complement(312..1166)
                     /locus_tag="AC16CY_09500"
     CDS             complement(312..1166)
                     /locus_tag="AC16CY_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012529.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3354064.1"
                     /translation="MTCAICEDTGWKSITIDGVARVTRCECVRGKTLERRLSDARIPP
                     RYQRCTLDTFVTYPNEELLRAVESAKRFARAFPVVQKGLMLIGPPGIGKTHIAVSALK
                     HVIDRTGAKGLYYDTRALLRDIRNTYNPVTHTAEMDVIRPVMDAELLVLDDLGAERLT
                     DWVEETMSLIVNTRYNERRATIFTSNYEDLPDEGELNSLLMRVGFRLHSRLREMCEFL
                     EYDGPDYRNLPPNGGADELYMLWKAQPRRKLPARAQGQARAQLKTRKQDHPDKVDLKW
                     SGGRAGSS"
     gene            1318..2370
                     /locus_tag="AC16CY_09505"
     CDS             1318..2370
                     /locus_tag="AC16CY_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354065.1"
                     /translation="MHHAPVTRRLPLAALLAALVSTVTLMAATLGGPLPAPLPLFPRD
                     NWWNIDISAAPLDPSSSSYINFINNGSTRRLHPDFGGEASPGSVEIYGFPYVVVDGNE
                     PKQPVYFQYDDESDGVDHTTGQSYPFYPIPAEAITTPHWVEGGEPGNVDRRSSDRHLI
                     IVDRDNRHLYELFNVFYDGTRWLAGSGAFFDLSTNNRRPEGWTSADAAGLAILPGLVR
                     YDEAFGSSDIQHAFRVTVRSTNGYVYPASHRAGSTIGAPPMGARLRLKSTVSLSGYTP
                     EIQRIFRAMQRYGLIVADNGSDMYISGTFDLRWNNSVLNPAFRSLTASDFEVVQLGWR
                     PQGLPPGAPANVRITP"
     gene            complement(2394..2798)
                     /locus_tag="AC16CY_09510"
     CDS             complement(2394..2798)
                     /locus_tag="AC16CY_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3354066.1"
                     /translation="MISAVSQKYDIHPQTLRLYEREGLLKPSRTEGNTRLYSEADLEQ
                     LETILTLTRDLGVNLAGVEIILNLRRKIEQMQGEVNLFMEYVKRELARGIDDWEQRLG
                     TALVKSAPTDLVRHVPPSPPDLDEGEPKRKKS"
     gene            complement(2841..3908)
                     /gene="dnaJ"
                     /locus_tag="AC16CY_09515"
     CDS             complement(2841..3908)
                     /gene="dnaJ"
                     /locus_tag="AC16CY_09515"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3354067.1"
                     /translation="MDLYIVLGVAHGATEAEIKRAYRRLARRYHPDINPGDQSSAARF
                     RQILEAYETLIDPTRRSRYDAGHATADRASARSGFEGFDFSTRGVDYSATFGDLFADV
                     LTERSTRQAPPQRGADLHVEMQVSFEEALSGGRRAVTVTRREHCRSCTGSGRTKVSTG
                     TCPLCQGSGAVRSVRGHMVFSRSCGACGGTGQQRPRACEPCGGTGQETRSETVTARIP
                     AGVADGDRLRVPGKGHAGAAGGPAGDLYITARVAAHPLFRREGDDLHMVVPVAVHEAA
                     LGARIEVRTLDGAARLRVPQGTQSGQRFRLRGHGAPSTRDGQRGDLIVEIRIMLPKLL
                     DERSKELLREFGRINVEPVRE"
BASE COUNT          640 a         1401 c         1267 g          701 t
ORIGIN      
        1 tcagacagcg cgaacgactg ccggcacgcc tcgacgagac gcccgatttc gcgcggctcg
       61 agcagagacg gcgttccgcc gccgaaatag accgtgtcgg ccgccgcccc gtccgctttc
      121 gcgtgaacct ccgtgagcag cgcggcgatg tactgctcct tcagggcggc gtcgtacaag
      181 ccgcggttga agttgcagta gttgcagata gaggagcaga acggaatgtg gaggtagagc
      241 ccgagcgccg ggctgtgacc gttgaccatt gcactttttc gtacgtagtg tccggcttca
      301 gccggacctg attacgagga tcccgcccgg ccccctgacc acttcagatc gactttgtcc
      361 ggatggtcct gctttcgcgt cttgagttgc gcgcgggcct ggccctgggc acgagccgga
      421 agcttgcgcc ggggttgtgc cttccaaagc atgtagagct cgtcggcgcc cccgttgggc
      481 ggaaggttcc tgtagtcggg accgtcatac tcgagaaact cgcacatttc cctcagccgc
      541 gagtgcagac ggaacccgac acgcataagc agcgaattca gctcaccctc gtctggcagg
      601 tcttcgtagt tcgaggtgaa gatcgtggcc cggcgctcgt tgtacctcgt gttgacgatg
      661 aggctcatcg tctcttcaac ccagtcggtg agacgctcgg caccgagatc gtcgagcacc
      721 aggagctctg catccatcac gggtcggatg acgtccatct ctgcggtgtg agtgaccgga
      781 ttgtacgtgt tgcggatgtc gcgcaatagc gcccgggtgt cgtagtacaa ccccttggct
      841 cccgtccggt cgattacatg tttcagcgcc gagacagcga tgtgcgtctt gccgattcct
      901 ggcggcccga tgagcatcag acccttctgg acgacgggaa acgcccgcgc gaagcgctta
      961 gctgattcga ccgcgcgaag caactcttcg ttgggatacg tcacgaaggt gtcgagcgtg
     1021 caccgctggt aacggggagg gatgcgtgcg tcgctcagac ggcgctcgag cgtctttccg
     1081 cgtacgcact cgcatctggt tactcgcgcg acaccgtcga tggtgatcga cttccagccc
     1141 gtgtcctcgc agatcgcgca cgtcacgcga catggtaact cgaatcgacg cgagacggcg
     1201 cagcgcctca ggaaccgaaa tcgcggtcga atgacagaac tgtcggccgt gcgcgatctc
     1261 ctcgcgagat ttgcgagcaa agacgtcagg cacgtcggtt gcctcactcg tcgccgcatg
     1321 caccacgcac ctgtgacgcg ccgcctgccg ctggcagcgc tcctggcggc gctcgtctcg
     1381 acggtcacgc tgatggcggc gacgctcggc ggcccgctgc ccgcgccgct gccgctcttt
     1441 ccgcgcgaca actggtggaa catcgacatc tccgccgcgc cgctcgatcc ctcgtcctcc
     1501 agctacatca acttcatcaa caacggctcg acccgccggc tgcatcccga cttcggcggc
     1561 gaggcctccc cgggcagtgt cgagatctac ggcttcccgt acgtcgtcgt ggacggcaac
     1621 gagccgaagc agccggtcta ttttcagtat gacgacgaga gcgacggcgt ggaccacacc
     1681 acgggacaga gctacccgtt ctatccgatc ccggccgaag cgatcaccac gccgcactgg
     1741 gtcgagggcg gcgagcctgg caacgtcgac cgtcgatcct ccgatcgcca tctgatcatc
     1801 gtggaccgtg acaaccggca cctgtacgaa ctgttcaacg tcttctatga cggcacgcgc
     1861 tggctggctg gctccggtgc gttcttcgat ctcagcacga acaaccggag gccggagggc
     1921 tggacgtcgg cggatgcggc ggggctcgct atcctgcccg ggctcgtgcg gtatgacgag
     1981 gcgttcggat ccagcgacat ccagcacgcc tttcgcgtca cggtcagatc gacgaacggc
     2041 tacgtgtatc cggcctcgca cagggcgggc tccacgatcg gcgcgccccc gatgggcgcc
     2101 cgcctgcggc tcaagtcaac agtgagcctg tccggctaca cgccggaaat ccagcgaatc
     2161 ttccgggcga tgcagcgcta cggcctgatc gtcgccgaca acggttcgga catgtatatc
     2221 agcggaacgt tcgacctgcg atggaacaac agcgtgctga acccggcgtt ccgctcgctg
     2281 accgcaagcg acttcgaggt cgtacagctc ggatggcggc cgcagggtct gccgccgggc
     2341 gcgcccgcca acgtcaggat caccccctag aggcctcacg cgggcctggc ggctcagctc
     2401 ttcttgcgct tgggctcgcc ttcatcgagg tcgggaggcg acggcggcac gtggcgcacg
     2461 aggtcagtgg gcgccgactt gacgagcgcc gtacccaggc gctgctccca gtcgtcgatg
     2521 ccccgggcca gctcgcgctt gacgtactcc atgaacaggt tgacctcgcc ctgcatctgc
     2581 tcgatcttcc gacgcaggtt gaggatgatc tccacgccgg ccaggttcac gccgaggtcg
     2641 cgagtcagcg tcaggatcgt ctcgagctgc tcgagatcgg cctccgagta caggcgggtg
     2701 ttgccttcgg tccgcgacgg cttgagcagc ccctcgcgct cgtagaggcg gagcgtctgc
     2761 gggtggatgt cgtacttctg cgacacggcg ctgatcatgt agtacgcctt gccgcgttcg
     2821 ccgctgcgct ttgccacgtg ttactcccgc accggctcca cgttgatccg tccgaactcc
     2881 cgcagcagct ccttcgaacg ctcgtcgagc agcttcggca gcatgatgcg aatctcgacg
     2941 atcaggtcgc cccgctgtcc gtcccgcgtg gacggcgctc cgtgcccgcg caggcggaac
     3001 cgctgcccgc tctgggtccc ctggggcacg cgcagccgcg ccgcgccatc cagcgtgcga
     3061 acctcgatgc gtgcgccgag cgccgcctcg tggaccgcaa cgggcaccac catgtgcagg
     3121 tcgtctccct cccggcggaa cagggggtga gccgcgaccc tcgccgtgat gtacaaatcc
     3181 ccggcggggc cgccagccgc gcccgcgtga cccttaccgg gcacgcgcag gcgatcgccg
     3241 tcggccacgc ccgccggaat gcgcgccgtg accgtctcgc ttctcgtctc ctggccggtg
     3301 cccccgcacg gctcgcacgc cctcggccgc tgctgccccg tcccgccgca ggcgccgcag
     3361 ctgcgcgaga acaccatgtg ccctcgcacc gatcggacgg cgcccgaacc ctgacagagc
     3421 gggcacgtgc cggtgctcac cttcgtccgg ccgctgcccg tgcacgaccg gcagtgctcg
     3481 cgccgcgtca cggtcaccgc ccgccgcccg cctgacagcg cttcctcgaa gctgacctgc
     3541 atctccacgt ggagatcggc cccccgctgc ggcggcgcct gcctggtgct ccgctcggtc
     3601 agcacgtcgg caaacaggtc gccgaacgta gccgagtagt ccacgccgcg cgtcgagaaa
     3661 tcaaagccct cgaagccgct tcgcgcgctg gcgcgatcgg cggtggcatg ccccgcgtcg
     3721 taccgcgacc gacgtgtggg atcgatgagc gtctcgtacg cttccaggat ctgacggaac
     3781 cgcgcggccg acgactggtc gcccgggttg atatccgggt ggtagcgacg ggcaaggcga
     3841 cgataggccc gcttgatctc ggcctccgtt gcgccgtgcg ccacgcccaa cacgatgtac
     3901 agatccattg gccgatttac ttgtcgtcca cgacctcggc gtcgatgacg tcggagccgc
     3961 cgccaggcga acccccggaa cccgttgaac cctgcgaacc cggtgcacc
//
LOCUS       DBNGSH010000417         3183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4282663, whole genome shotgun sequence.
ACCESSION   DBNGSH010000417 DBNGSH010000000
VERSION     DBNGSH010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4282663"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1031)
                     /gene="mch"
                     /locus_tag="AC16CY_09520"
     CDS             complement(60..1031)
                     /gene="mch"
                     /locus_tag="AC16CY_09520"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR03120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HIR3354068.1"
                     /translation="MNGEAVDLGMNERAWAIADRAAERSGELRIDVHALACGARVIDA
                     GVQAPGGLAAGLMLAELCMGGLGHVEHASLTIGGDTWPGLRVWTDHPAVSCMASQYAG
                     WAINPEGYFAMGSGPLRARARVEKELFGTLEYSEDASRGVLVLEGRTLPTDDVAQWVA
                     RKSGVAAGCLTFAIAPTASLAGGVQIAARSIETGLHKMHTLGFDVRKVVSAIGTAPLP
                     TLAKNDVRAIGRTNDCVLYGAQARYMVRADDEELAALAARLPASTSSDYGTPFYDIFK
                     RYDHDFYKIDPMLFSPAEVWLTCAASGRTFHAGGLNADVLRASLYGS"
     gene            1211..>3183
                     /locus_tag="AC16CY_09525"
     CDS             1211..>3183
                     /locus_tag="AC16CY_09525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3354069.1"
                     /translation="MTSRRFSVRFSRATLLPILLASLVLGAACSRTSTPATAAVTEAD
                     VPLIPRRLLFGNPERASPSLSDDGKQLAFRAEVNGVMNVWVAPADKPGDARAVTKDTS
                     RGIRQYFWAYTNSHIVYLQDQGGDENWRVYSVDLASGATKDLTPFEKVAAQIQEVSRR
                     FPEEILVSLNNRNPQFHDVYRVNIVTGARTLLQRNDAYAGFTTDDSYKVRFAYQFRPD
                     GGLDVLRRTDAGRFEPFEQIGPDDALTTQIIGFDKTAQQIYMVDARNRDTAALFRKDL
                     ATGTQTLLAEDPKSDAQNGLIHPVEKTVQAVGFNYDRVRWQVLDPAIGEDLKYLQSVT
                     DGEIEIGSRTSDDSRWIVAYLKDDGPVRVYLYDRPARMATFLFTNRPALEKSKLARMH
                     PREIKSRDGLMLVSYLSLPVWTDPDNDGRPAAPLPTVLLVHGGPWGRDTWGYDSIHQL
                     LTNRGYAVLSVNFRASTGFGKKFLNAGNKEWGGKMHDDLVDAVNWAVASGVADAKKTV
                     IMGGSYGGYATLVGLTSTPDLFAAGVDIVGPSNLVTLLNTIPPYWAPQIELFTSRVGD
                     HRTPDGRKLLESRSPLNQVDRISKPLLIAQGANDPRVKQAESDQIVKAMQARKIPVTY
                     ALFPDEGHGFARPENNVAFMAIAEAFLARHLGGR"
BASE COUNT          549 a         1109 c         1036 g          489 t
ORIGIN      
        1 cgcaggagct cggtcgtatg cccgcccgcg cgtgcgctga ggatcacggc gcgcatccgt
       61 cagctcccgt acagcgaagc gcgcagcacg tcggcgttga gtccgcctgc gtggaacgtt
      121 cgtccgctcg ccgcgcacgt cagccagact tccgcggggc tgaagagcat cgggtcgatc
      181 ttatagaagt cgtggtcgta gcgcttgaag atgtcgtaga acggcgtgcc gtagtcggaa
      241 gaggtcgacg ccggcagacg cgccgccagc gccgccagtt cctcgtcgtc ggcgcgaacc
      301 atgtagcgcg cctgcgctcc gtacaggacg cagtcgttgg tccggccgat ggcccggaca
      361 tcgtttttcg caagcgtcgg cagcggcgcc gtgccgatcg cgctcacgac cttcctgacg
      421 tcgaagccga gggtgtgcat cttgtgaagc cccgtctcga tgctgcgggc cgcgatctga
      481 acgccgccgg cgaggctggc cgtcggggcg atcgcgaagg tgaggcagcc ggccgcgacg
      541 ccggacttgc gagccaccca ctgggccacg tcgtcggtcg ggagcgtccg cccttccagc
      601 accagcacgc cgcgcgatgc gtcctccgag tactcgagcg tgccgaagag ctccttctcg
      661 acgcgcgccc tggcgcggag cggccccgac cccatcgcga agtaaccctc ggggttgatg
      721 gcccagccgg cgtactgcga tgccatgcat gacacggccg gatgatcggt ccacaccctg
      781 aggcccggcc acgtgtcgcc gcctatcgtc aaagacgcat gctcgacgtg accgaggccg
      841 cccatgcaca gctcggcgag catcagtccg gccgcgagac caccgggcgc ctgcacgccc
      901 gcgtcgatca ctcgtgcgcc gcatgcgagc gcgtgaacgt cgatgcgcag ttcgccagac
      961 cgttccgccg cgcgatcggc gatggcccat gcgcgctcgt tcatcccgag atcgacggct
     1021 tccccattca cgcgcgagca tcctcctcgc aacagcagta gaggcgggtc tttagacccg
     1081 ccttgctgca cccagcccag gcgggtctga agacccgcct ctacgccaca cagtcgaact
     1141 acggtgtgga gccgccttgc agtctatact gtggcgccgc accctccgtc ttcagccgaa
     1201 ggagtctccg atgacctcgc gccggttcag cgtccggttc tcacgcgcaa cattgctgcc
     1261 gatcctgctc gcgagcctgg tgctcggagc cgcctgcagc cgcacgagca cgccagcgac
     1321 ggcggcagtg accgaagcgg acgtcccgct gattccccgc cggctgctgt tcggaaatcc
     1381 ggagcgcgcc agcccgtcgc tcagcgacga cggcaagcag ctcgccttcc gggcggaggt
     1441 caacggcgtg atgaacgtct gggtcgcgcc ggccgacaag cccggcgacg cccgcgccgt
     1501 gacgaaggac acgtcgcgag gcatccggca gtacttctgg gcctacacca actcgcatat
     1561 cgtctatctg caggatcagg gcggagacga gaactggcgc gtctactcgg tggacctggc
     1621 ctccggcgcc accaaggatc tgacgccgtt cgagaaggtc gccgcgcaga tccaagaggt
     1681 gagccggcga ttccccgagg agattctcgt ctcgctcaac aacaggaacc cgcagttcca
     1741 cgacgtctat cgcgtcaaca tcgtcacggg cgcgcgcacg cttctgcagc ggaacgacgc
     1801 gtacgccggg ttcacgaccg acgacagcta caaagtgcgg ttcgcctacc agttccgccc
     1861 cgacggcggc ctcgacgtgc tgcgccggac cgacgcgggc cgcttcgagc cgttcgagca
     1921 gatcggacct gacgatgcgc tgaccacgca gatcatcggg ttcgacaaga ccgcgcagca
     1981 gatctacatg gttgacgcgc gcaaccggga cacggccgcg ctcttccgga aggacctcgc
     2041 cacgggcacg cagacgctgc tggccgagga cccgaaatcc gacgcgcaga acgggctgat
     2101 ccacccggtc gagaagacgg tccaggccgt gggcttcaac tacgaccgcg tgcgatggca
     2161 ggtgctcgac ccggccatcg gcgaggactt gaagtacctg cagagcgtca ccgacggcga
     2221 gatcgagatc gggtcgcgta cgagcgacga ctcgcgatgg atcgtggcct acctgaagga
     2281 cgacgggccg gtgcgtgtct acctgtacga ccgccccgcc cgcatggcga cgttcctctt
     2341 caccaaccgc cccgcgctgg agaagtcgaa gctcgcacgc atgcacccgc gcgagatcaa
     2401 gtcacgcgat ggcctgatgc tcgtcagcta tctctcgctc ccggtatgga cggatccgga
     2461 caacgacggc cgtcccgccg cgccgcttcc gacggtcctg ctggtgcatg gcggaccatg
     2521 gggacgcgac acctggggct acgacagcat ccaccagctc ctgaccaatc gcgggtacgc
     2581 cgtactctcc gtcaacttcc gcgcatcgac cggcttcggg aagaagttcc tgaacgccgg
     2641 caacaaggag tggggcggga agatgcacga cgacctggtg gacgcggtga actgggcggt
     2701 cgcatcggga gtcgccgacg cgaagaagac cgtgatcatg ggaggctcct acggcggcta
     2761 tgcgacgctc gtcggcctca cctcgacgcc tgacctcttc gcggccggcg tggacatcgt
     2821 cggcccgtcg aacctcgtga cgctgctgaa cacgattccg ccgtactggg cgccgcagat
     2881 tgagctgttc acctcgcgcg tcggcgacca ccggactccg gatgggcgca agctgctgga
     2941 gtcgcgttcg ccgctgaacc aggtggaccg catctcgaag ccgctgctca tcgcgcaggg
     3001 ggcgaacgat ccgcgcgtga agcaggccga atcggaccag atcgtgaaag cgatgcaggc
     3061 caggaagatc cccgtgacct acgcgctttt cccggacgag gggcacgggt tcgcgcgtcc
     3121 cgagaacaac gtcgcgttca tggcgatcgc cgaggcgttc ctcgcgcgtc atctcggcgg
     3181 ccg
//
LOCUS       DBNGSH010000418         6161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4282671, whole genome shotgun sequence.
ACCESSION   DBNGSH010000418 DBNGSH010000000
VERSION     DBNGSH010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4282671"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="AC16CY_09530"
     CDS             <1..205
                     /locus_tag="AC16CY_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3354070.1"
                     /translation="HNVTHRVMLNDGSIDTGDIAPGATSRAVQMPAEGTNYHCGVHPG
                     MIGAVSSSAGPPPVCQGIYCDPY"
     gene            complement(303..1001)
                     /locus_tag="AC16CY_09535"
     CDS             complement(303..1001)
                     /locus_tag="AC16CY_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR3354071.1"
                     /translation="MTKTSLLLSVAGALLLLAGPLGTRVGVWPFVVGFLMLAVSLLLG
                     LAGASLALVAGVKTGSWAPALAGILAGLAVVSVPSFIVVSARGTPPIHDITTDPDDPP
                     LFVAILPLRAGAANPPEYGGAQVADQQRAAYPDVKPLVLQTDAGRAFDRAMTVVRDLG
                     WDVVADDKSSGRIEAVDTTFWFGFKDDVVVRVREEAGRARIDVRSKSRVGVGDVGTNA
                     RRVRELLRGIAESK"
     gene            complement(1084..1503)
                     /locus_tag="AC16CY_09540"
     CDS             complement(1084..1503)
                     /locus_tag="AC16CY_09540"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354072.1"
                     /translation="MNTRTGALFGLTLALMSSGDVQAHHSFAAQYDANKPVTLVGTVT
                     KLEWTNPHARFYVDVKDDKGVVTNWNLELASPNVLRRNGWSRTSLKVGDEVTVEGSAA
                     KNGTKMANARVVTLASGQRVFAGSSGGDAPPPSQAKP"
     gene            1618..3129
                     /locus_tag="AC16CY_09545"
     CDS             1618..3129
                     /locus_tag="AC16CY_09545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354073.1"
                     /translation="MTGVRGLAVAGMGLLAAACSSAPTARGLAQEAVNAMGGADRLRA
                     VQTLVMSGGAGTRARLGQTTRVGDPETAAKLVNVIETLDLAGGRAALDYQIELDGFMQ
                     HRQEVLTKKDGKAVGLENVAGRPLAVVSPSGLFSWGSQNSPEFLLRRNVITVALAAAQ
                     SAPEEPAQDRTLGLRTLKAAAATLPSGESATVFFEPDSKLLAGYEVLDTESMLGDVTA
                     QYILSDYKPAGDLMLPHKITIRKGGQPYAEVQFASASVNDAVALEVFEIPASANQAAD
                     TAIAAGDYSPIAIDKVAPGVYFARAYSHNSLVVEFPSWLAVVEAAYTDAQSHTLARVL
                     QQQFPGKPIRYAAVTHHHYDHTGGVRTLASYGATILVEKGHEPEMRMILETPHTNPPD
                     ALETKRKAGQTVGAIEVFEGKKVLSDGGQSLELIAISNPHVEPKVIAFVPKGGVLFQS
                     DIFFPGLGAPASPAAVQLLASVRSLKLRVMTNAGGHGGVGPFAELEKAVAAVK"
     gene            3178..>6161
                     /locus_tag="AC16CY_09550"
     CDS             3178..>6161
                     /locus_tag="AC16CY_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3354074.1"
                     /translation="MGTIAGRYQVTAKLGEGGMGVVFRAYDPPPMDREVAVKTLHEFT
                     DPLALELFYKECSALKSISHPNIVEIFDMGEFEEEGHRKPYFVMPLLPGQTLDKLIKD
                     SSHRLTVERVVEIFAQTCRGLQAAHEHGLVHRDLKPSNVFVMADDSVKIIDFGVAHAV
                     TMESRSTGFDKGTLLYMAPEQVQHKPVSVQSDIYSLGVTLYEALTRRQPFRAASEQAV
                     VHAILTQIPPPASDLNPAVSHAISRVVHKAMAKQPWNRFDTARELGDTLQKAARNEPI
                     AIFDPARTQPRIQTAMKALDKGDYQFAAEIVGELEAEGNIDPQITLLRTQIDQIARQK
                     TIAQLLESARARYEEEEDPLALSKLQEVLQLDPTNVAALGLKSKIEDRRSERQIEKWI
                     QLARQHVDNHSYGHAREALQNALVLRPKDPRATKLLKEIEGEEQEYVRLRREKTQMYE
                     SAVNAWKNGEISQALSQMRRVLDLDVKAPDASSPDAAGAYQSFFDKVRSEHDAMNAGY
                     ADAKRHLAEKRFADALRICTDFLAKYPNQALFQALKFDIDEQQRQQLSAFIAEVDRRL
                     EAEPDLEAKVALLREAVAAYPDEEHFRRLAGVIEDKRTLVNSIVERARVHEAASQISE
                     ALADLDTLRTIYGIYPGLSFERERLQKRLEQQTRDAARTNWARQIDAQLEVGNYARAH
                     ELLDMAEQEFAGEAELAELRKLAVQGQERLARADQLVADGQQLCAQGDFDQGVDLLKI
                     ALQLDDRPAVRQTLRDLFVTKAQEGLDSDWRTVEAFAERALELDPDHALAKSLRAQAL
                     DRKREEDVPRYASQARRLQAAGDLTGAMTEVEKGLSSYPGDPRLSAIADAVRKELDRT
                     APPPAPAVTAPEPAAPSLERTRLVIDRRRTPPEATAPPEAPTAAPMPTPAPVPAPKPA
                     PALRPRSGSHRLLWGALAGLAVVLLAVVVFNILGGPGEDVSQAGAPVAPASEPAATPP
                     PATPAAPATPASLALQQLPPGL"
BASE COUNT         1109 a         2088 c         2092 g          872 t
ORIGIN      
        1 ccacaacgtg acgcaccgag tgatgctcaa cgacggatcc atcgacacag gcgacatcgc
       61 tccgggagcg acgagccgcg ccgtccagat gccagcggaa ggaacgaact accactgcgg
      121 cgttcatccg ggaatgattg gcgcagtgtc gtcctcggcc ggcccgccgc ccgtctgcca
      181 gggaatctac tgcgacccgt actgaggtcg cattcgacaa ccggggccgg acccgacccg
      241 cccgcccccg ggtccgccgg acgctacgtg aactccgccc cgggtccggc tgaagccgga
      301 cactacttgg actctgcgat cccccgcagc agttcgcgca cgcggcgggc gttggtgccg
      361 acgtcgccga cgcccacgcg cgatttcgat cgcacgtcga tccgtgcgcg gcctgcttcc
      421 tcccgcacgc gcacgacgac gtcgtccttg aagccgaacc agaacgtcgt atcgacagct
      481 tcgattcgtc cggatgattt gtcgtcggcc acgacatccc agccgagatc gcggacgaca
      541 gtcatggcgc gatcgaacgc ccgtccggcg tccgtctgaa ggacgagcgg cttgacatca
      601 ggataggcgg cgcgctgttg atccgccacc tgagcgccgc cgtactccgg cggattcgcg
      661 gcgcccgcgc gcaggggcag aatcgcgacg aacaacggcg ggtcgtcggg atcggtggtg
      721 atgtcatgga ttggcggcgt gccgcgcgcc gacaccacga taaagctcgg tacggagacg
      781 acggccagcc ccgcgaggat gccggccaga gccggcgccc acgatccggt cttgacaccg
      841 gcgacgagcg cgagcgacgc gccggcgagc ccgagcaaca ggctgacggc gagcatcagg
      901 aagccgacga caaacggcca cacgccgacg cgggttccga gcgggccagc cagcaacagc
      961 agggcaccag cgacggacag aagcaaactc gtcttagtca cgggcacttc gcatcagtcc
     1021 cgagcccgaa cgtgaacacg aaccgagaag tgagaagcga gaagggtgaa ccggaggatt
     1081 ctcctatggt ttggcctgcg acggcggggg cgcatcgcca ccggaggacc ccgcgaagac
     1141 acgctggccg ctggcaagcg tgacgacgcg ggcgttggcc atcttggtgc cgttcttggc
     1201 ggccgaacct tcgacggtga cttcgtcgcc gaccttcagc gaggtcctgc tccacccgtt
     1261 gcgccgcagg acgttcgggc tggcgagctc gaggttccag ttcgtgacca cgcccttgtc
     1321 gtctttcacg tccacgtaga agcgtgcgtg cgggttggtc cactcgagct tggtgaccgt
     1381 gcccaccagc gtgaccggct tgttcgcgtc gtactgcgcc gcaaacgaat ggtgcgcctg
     1441 gacgtcccct gaagacatca gcgcgagtgt cagtccaaac agggcgccgg tacgtgtgtt
     1501 catgagtgcc ctccacggat cgccgcgcgg tctgcggtcc ctcgcggcga atggtacggc
     1561 gcgcgccgtt cacctgtaaa gacctgatag agtagcggcg ctcgaggagg aatgcacatg
     1621 acgggagttc gaggactggc tgtcgcgggg atggggcttc tggccgccgc atgctcaagc
     1681 gcgccgaccg cgcgcgggct cgcgcaggag gcggtgaacg ccatgggcgg ggccgaccgg
     1741 cttcgcgccg tgcagacgct cgtgatgagc ggcggcgccg gcacgcgcgc ccgtctggga
     1801 cagacgactc gcgtcggaga tccggagacc gctgcgaagc tcgtcaacgt gatcgagacg
     1861 ctcgatctcg cgggagggcg ggcagcgctc gactatcaga tcgagctcga cggcttcatg
     1921 cagcaccggc aggaggtcct caccaagaag gacggcaagg cggttggcct cgagaacgtc
     1981 gcgggccgcc cgctggcggt cgtgtctcca tcgggcctct tcagctgggg ctcgcagaac
     2041 tctccggagt tcctgctccg ccgcaacgtg atcaccgtgg cgctcgccgc ggcgcagtcg
     2101 gctccggagg agcccgcgca ggacaggacg ctcggactgc gcacgctgaa agcggcggcg
     2161 gcgacactgc cctccggcga gtcggccacc gtcttcttcg agccggactc gaagctgctg
     2221 gccggctacg aagtgctcga caccgagtcg atgctgggtg atgtcacggc gcagtacatc
     2281 ctgagcgact acaagccggc tggcgacctg atgcttccac acaagatcac catccgaaag
     2341 ggcggccagc cgtacgccga ggtgcaattt gcgtcagcat cggtgaacga cgctgtcgcg
     2401 cttgaggtct tcgagattcc cgcgtcggcg aaccaggcgg ccgatacggc cattgccgcg
     2461 ggcgactact cgcccatcgc gatcgacaag gtcgccccgg gcgtctattt cgcacgcgcc
     2521 tactcgcaca acagcctggt ggtcgaattt ccgtcgtggc tggcggtggt cgaggccgcg
     2581 tacaccgacg cgcagagcca cacgctcgcg cgggtgcttc agcagcagtt cccgggcaag
     2641 ccgatccgct atgcggcggt gacacaccat cactacgacc acacgggcgg cgtgcgcacg
     2701 ctggcgtcct acggggcgac gatcctggtc gagaagggac acgagcccga gatgcgcatg
     2761 atcctcgaga cgccgcacac caatcctccc gacgcgctcg agacgaagcg caaggccgga
     2821 cagaccgtgg gcgcgattga agtcttcgag gggaagaagg tactgagcga cgggggccag
     2881 agcctcgagc tcatcgccat cagcaacccc cacgtggagc cgaaggtgat tgccttcgtg
     2941 ccgaagggtg gggtgctctt ccagagcgac atcttcttcc ctggcctcgg cgcgccggca
     3001 tctccggcgg cggtgcagct gctggcatcg gtgcgcagtc tcaagctgcg cgtgatgacg
     3061 aatgccggcg gccacggcgg agtcgggccg ttcgccgagc tcgagaaagc agtcgcagcc
     3121 gtgaagtgac gcacccgggc gcgggcgacc ttcacgctag aatgaaggtc tgtgcccatg
     3181 ggaacgatcg cagggcgcta tcaggtcacg gccaagctcg gcgaaggtgg aatgggcgtg
     3241 gtgttccgag cctacgaccc gccgcccatg gaccgggaag tcgccgtcaa gaccctccac
     3301 gaattcaccg atcccctcgc gctcgagctc ttctacaagg agtgctcggc gctcaagtcg
     3361 atcagccacc ccaacatcgt cgagatcttc gacatgggcg agttcgagga agaagggcac
     3421 cggaagccct acttcgtcat gccgctcctg cccggacaga cgctcgacaa gctgatcaag
     3481 gactcgagcc accggctgac cgtcgagcgc gtggtggaga tcttcgcgca gacgtgccgg
     3541 ggcctgcagg ccgctcacga gcacgggctc gtccaccgcg acctgaagcc gagcaacgtg
     3601 ttcgtgatgg cggacgactc ggtgaagatc atcgacttcg gcgtggcgca cgctgtgacg
     3661 atggagtcgc gatcgacggg cttcgacaag ggcacgctgc tctacatggc gccggagcag
     3721 gtgcagcaca agcccgtgtc ggtccagtcc gacatctact cgctgggcgt caccctctac
     3781 gaggcactga cgcgcaggca gccgttccgc gccgcgagcg agcaggcggt cgtccacgca
     3841 atcctgacgc agattcctcc gcccgcctct gacctcaatc ccgccgtcag ccacgccatc
     3901 agccgcgtcg tgcacaaggc catggccaag cagccctgga accggttcga cacggcgcgc
     3961 gagctcggcg acacgctgca gaaggccgcg cgcaacgagc cgatcgccat cttcgacccc
     4021 gcgcggacgc agccgcgcat ccagacggcc atgaaggcgc tcgacaaggg cgattatcag
     4081 ttcgccgcag agatcgtcgg cgagctcgag gccgaaggga acatcgatcc gcagatcacg
     4141 ctcctcagga cccagatcga ccagatcgcc cgccagaaga cgatcgcgca gctcctcgag
     4201 agcgcccgcg cccgctacga ggaggaggag gatccgctcg cgctgtcgaa gctccaggaa
     4261 gtcctccagc tcgacccgac caacgtcgcg gcgctcgggc tcaagagcaa gatcgaggac
     4321 cgccggagcg agcgccagat cgagaaatgg atccagctcg cgcgacagca cgtggataac
     4381 cattcctacg ggcacgcgcg cgaggcgctg cagaacgcgc tcgtgctccg cccgaaggac
     4441 ccgcgggcca cgaagctcct caaggagatc gagggcgaag agcaggagta cgtccgcctg
     4501 aggcgcgaga agacgcagat gtacgagtcg gcggtcaacg cctggaagaa cggcgagatc
     4561 agccaggcgc tcagccagat gcggcgggtg ctcgatctcg acgtgaaggc ccccgacgcc
     4621 tcgtcgcctg acgcggccgg ggcgtaccag agcttcttcg acaaggtccg ctcggagcac
     4681 gacgcgatga acgccggcta cgcggacgcg aagcgtcacc tggccgagaa gcgattcgcg
     4741 gacgcacttc ggatctgcac ggacttcctg gcgaagtacc cgaaccaggc cctgttccag
     4801 gccctcaagt tcgacatcga cgaacagcag cggcagcagc tgtcggcctt cattgccgag
     4861 gtggaccggc gtctcgaggc ggagccggat ctcgaagcga aggtggccct gctgcgagag
     4921 gccgtcgcgg cgtatccgga cgaagagcac ttcaggcggc tcgcaggggt aatcgaggac
     4981 aagcgcacgc tggtcaattc gatcgtggag cgggccagag tgcacgaggc cgccagccag
     5041 atcagcgagg cgcttgccga cctcgacacg ctgcggacga tctacggcat ctaccccggg
     5101 ctatcgttcg agcgggagcg tctccagaag cggctcgagc agcagacgcg cgacgcggca
     5161 cgcacgaact gggcccggca gatcgacgcc cagctcgagg tgggaaacta cgcgcgcgcc
     5221 cacgagctgc tcgacatggc ggagcaggag ttcgccggcg aggccgagct ggcggagctg
     5281 cgcaagctgg ccgtgcaggg gcaggagcgc ctggcgaggg ccgatcagct cgtggccgac
     5341 ggacagcagc tgtgcgcgca gggagatttc gaccagggcg tggacctgct caagatcgcc
     5401 ttgcagctcg acgaccggcc ggccgtgcgg cagacgctgc gggatctctt cgtgacgaag
     5461 gcgcaggagg ggctggactc cgactggcgc acggtggagg cgttcgccga gagggcgctc
     5521 gagctcgatc cggatcacgc cctcgcgaag agcctccgcg cgcaggcgct cgaccggaag
     5581 cgcgaagagg acgtcccgcg gtatgcgtcg caggcgaggc gcctgcaggc ggccggcgac
     5641 ctgacgggcg cgatgacgga agtcgagaag gggttgtcct cctaccccgg cgatccgcga
     5701 ctcagcgcca tcgcggacgc cgtgcggaag gaactggatc gcacggcgcc accacctgct
     5761 ccggccgtga ccgcgcccga gcctgctgcg ccatcgctcg agcgcactcg actggtgatc
     5821 gaccgccggc gaacgccgcc ggaagcgact gctccaccgg aggcgcccac ggccgcgccc
     5881 atgccgacgc ctgcgcccgt gcccgcgccc aagcctgcac ccgcactcag gcctcggagc
     5941 gggtcgcacc ggctgctctg gggggcgttg gccgggctcg ccgtggtcct ccttgccgtc
     6001 gtcgtgttca acattctcgg cggtcctggc gaggacgtca gccaggccgg cgctccggtt
     6061 gctcccgcga gtgaaccggc agcgactcct ccgccggcga ctccggccgc gcctgccacg
     6121 cccgcctcgc tcgctcttca gcaactgccg cccgggcttc a
//
LOCUS       DBNGSH010000419         3555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_717659, whole genome shotgun sequence.
ACCESSION   DBNGSH010000419 DBNGSH010000000
VERSION     DBNGSH010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_717659"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="AC16CY_09555"
     CDS             complement(<1..1019)
                     /locus_tag="AC16CY_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR3354075.1"
                     /translation="MVGSFLVMAAATLLQQPSCDALKTLSLPHATITSAEFVAAGSAA
                     PGRGGRGGPGAPLPAHCRVAATLTPSADSHIEMELWLPTENWNGKFLALGNGGWAGTI
                     SLDAMAPALRRGYATASNDTGHKSGSASFVVGHPEKLIDFGYRAMHEMAVQSKAIIQA
                     FYKRGPQLSYYQGCSTGGRQGMMEAQRFPEDFDAIIAGAPVHNMVHLSVSQVALQVDM
                     LKNPSRLVPPEKRTLFANAVVAACDQQDGVKDGIVSEPRACKFDPAVLACKTADGPGC
                     LTASQVENARSAWAPVTRKNGTLVYPGRSPGFESGWRIPAPGAALNPLFTDMPRYVGR
                     QDPNW"
     gene            complement(1137..3221)
                     /locus_tag="AC16CY_09560"
     CDS             complement(1137..3221)
                     /locus_tag="AC16CY_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3354076.1"
                     /translation="MQVTGASGVVSAATSPVAFSETIAPTAISASYRLGLVLVTIAMI
                     LVPLLYLLLIGAVAWFLVWHLTANTWILSSGSGAQWRLLGYLTPALAGATLLFFMVKP
                     ILARRVRRGDPIQVTDAEQPALHALIREICHQVRAPLPSRVQVDCMVNASATLRGGGI
                     VGRQLDLTIGLPLVTGLSVRELSGVLAHEFGHFAQGGGMRMTALIRGINLWLYRVVHE
                     RDTWDSKLEEWSKEGDWRVVIPMALARLSVWASRQALAAVTMVGHGVSCYMLRQMEFD
                     ADSYEIKIAGTNAFTRTMVRLRELSAGSQMAYSRLKQSLDNRAIPEDLPLLFAEFSRR
                     LPDGIRERVQNVPDDPTGTFDTHPSDADRVAAAQVMAASGALVGGDEPATILFRAFDR
                     LSAVVTRHHYEDDLGLELDGIRLVAADEALRGAEDQRRTETAVRNFFNGRVSVTRPLR
                     ILVDDVATMEVADLDKALEESRAAMNASSVSDESYRAFEHYQVRRDLAFCAEHLFLAG
                     LPTVDAERFSLAEPTLDGAQSTQSWAQQEQDKLRADLDPFDALVARRLAIGALVLDRR
                     QRSDDARALTLAVNALAEAVPFTVDARRSTMAMVFLDEATPVLGTSPQLRARMASLAA
                     AVNECRTRAFEILGQATIPGGESGDAWQRLLADAPQLAPHDVLQRLMELYWGCLSQLA
                     RLVSQAEASTAA"
     gene            complement(3267..>3555)
                     /locus_tag="AC16CY_09565"
     CDS             complement(3267..>3555)
                     /locus_tag="AC16CY_09565"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564543.1"
                     /note="catalyzes the formation of protocatechuate from
                     4-hydroxybenzoate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HIR3354077.1"
                     /translation="DVLVLAAAMKAFYERGCTEGLDDYSGVCVRRVWNAQRFSWWMTS
                     TLHRFPDENAFDHQRQLADLDYLTGSRAAMTSLAEQYVGLPAEVPFESAGL"
BASE COUNT          627 a         1191 c         1157 g          580 t
ORIGIN      
        1 tcccaattgg gatcctgccg gccgacgtag cgcggcatgt cggtgaacag cgggttcagc
       61 gccgctcccg gtgccgggat gcgccagccg gactcgaaac ctggcgagcg tcccggatac
      121 acgagcgtcc cattcttcct cgtgaccggc gcccacgcac tccgggcgtt ctcgacctgt
      181 gaggcggtca ggcaacctgg gccatcggct gtcttgcacg cgagcaccgc gggatcgaac
      241 ttgcaggcgc gcggctcgct gacgatgccg tccttgacgc cgtcctgctg atcgcacgcg
      301 gcaacgaccg cgttcgcaaa cagcgtccgc ttctccggcg gaacgaggcg cgacggattc
      361 ttcagcatgt ccacctgcag cgcgacctgc gacacgctga gatgaaccat gttgtggacc
      421 ggcgcgccgg cgatgatggc gtcgaagtcc tccgggaagc gctgcgcttc catcatcccc
      481 tgccgcccgc ccgtggagca gccctggtag tacgacagct gcggaccgcg cttgtagaac
      541 gcctggatga tggctttcga ttgcacggcc atttcgtgca tggcacggta gccgaaatcg
      601 atcagcttct cgggatggcc gacgacgaac gaggcgctgc cacttttgtg gcccgtatcg
      661 ttcgaggccg tcgcatagcc gcgccgaagc gcgggcgcca tcgcgtcgag cgagatggtg
      721 cctgcccatc cgccgttgcc gagggccagg aacttgccgt tccagttctc ggtcggcagc
      781 cacagttcca tctcgatgtg ggaatcggcc gagggcgtca gcgtggccgc gacccggcag
      841 tgcgcgggca gcggcgcgcc cgggccaccc cggccgcctc gccccggcgc cgcgctgccg
      901 gccgcaacga attcagccga cgtgatcgtc gcgtgcggca gcgagagcgt cttgagcgcg
      961 tcgcatgacg gctgctgaag cagcgtagcg gcagccatca cgagaaagga accgaccatg
     1021 acgatctcct ccagcttcag ctcaaccagg cgcgcagcgc cggatgtggc tgggagatga
     1081 tactcaccat acactgccgc ctgcacgaac tctccgcagg catctccttc gaacggctac
     1141 gccgcagtcg atgcttcagc ttggctcacg agtcgcgcga gctgcgacag acacccccag
     1201 tacagctcca tcagccgctg caggacgtcg tggggcgcga gttgcggcgc atccgcaagc
     1261 agtcgctgcc atgcatcacc cgactcgccc cccgggatcg tcgcttgacc gaggatctca
     1321 aacgcgcggg ttcggcattc gttgacagcc gccgcgaggc tggccatccg cgcgcgcaac
     1381 tgtggagagg ttccgagcac cggagtggcc tcgtcgagga agaccatggc catcgtggag
     1441 cgccgagcgt ctacggtgaa cggcacggcc tcggccagcg cgttcaccgc gagcgtcaac
     1501 gcacgcgcat cgtcggaacg ctggcgtcgg tcgagaacga gcgcgccgat ggcgagtcgg
     1561 cgcgcaacca gtgcgtcgaa cggatcgaga tcggcacgca gcttgtcctg ttcctgctgc
     1621 gcccagctct gggtcgactg cgctccgtcg agcgtcggct ccgcaagcga gaaccgttca
     1681 gcgtcaaccg tcggcaggcc ggcgagaaac aggtgctcag cgcagaaagc cagatcgcgg
     1741 cgaacctggt agtgctcgaa ggcccgatac gattcgtcgg aaacactgga cgcgttcatg
     1801 gcggctcgcg attcctcgag ggccttgtca agatcggcga cttccatcgt ggcgacatcg
     1861 tcgacaagga tccgcagcgg acgtgtgacg ctgacgcgac cgttaaagaa gttgcggacg
     1921 gccgtttcgg tgcgccgctg atcttcggcc ccacgcagcg cttcatcggc tgcgacgagc
     1981 cggatgccat cgagctcgag gccaaggtcg tcttcgtagt gatgacgcgt gaccacggcc
     2041 gacaggcggt cgaacgcgcg aaacaggatc gtggcgggct cgtctccgcc cacgagcgca
     2101 ccgctcgccg ccatcacctg tgcggccgca acccggtcgg cgtctgacgg gtgtgtgtcg
     2161 aacgtcccgg tagggtcgtc agggacgttc tggacccttt cgcggatgcc atccggcagc
     2221 cgtcggctga attcagcgaa tagcagcggc aggtcttccg gaatggcccg gttgtcgagc
     2281 gactgcttca gtcggctgta cgccatctgc gagccggccg acagctcccg gagtcggacc
     2341 atcgttcgcg tgaacgcgtt cgttccggcg atctttatct cgtagctgtc ggcgtcgaac
     2401 tccatctgcc gcagcatgta gcagctcacg ccgtggccga ccatggtcac tgcggccagc
     2461 gcctgtcgcg acgcccacac cgacagacgc gccagcgcca tcgggatgac cacgcgccaa
     2521 tcgccttcct tcgaccattc ctccagcttg ctgtcccacg tgtcccgctc gtgcaccacg
     2581 cggtacagcc ataagttgat tcctcgaatc aacgcggtca tgcgcattcc cccgccctgc
     2641 gcgaaatgac cgaactcgtg tgcgagaacg ccgctcaact cgcgaaccga gagtccggtc
     2701 accagaggaa ggccgatcgt gagatcgagc tggcgaccga cgatcccgcc gccgcgcagc
     2761 gtggctgacg cgttgaccat gcaatccacc tggactcgtg acggaagtgg cgcgcgcacc
     2821 tggtggcaga tctcgcgaat gagcgcatgc agagccggtt gttcggcgtc agtcacctga
     2881 atcgggtcgc cgcgacgaac tcgccgggcc aggatcggtt tgaccatgaa aaacagcaac
     2941 gtggcgccgg cgagggccgg cgtgagatag cccagaagcc gccactgcgc gcccgacccg
     3001 ctggagagga tccaggtgtt cgccgtcagg tgccagacga ggaaccacgc cacggcgccg
     3061 atgagtaaca ggtacagcag gggcaccagg atcattgcga ttgtgacgag caccaggccc
     3121 agacgatacg acgctgagat ggcggttggg gcaatggtct ccgagaaggc aacgggactt
     3181 gttgcggcgg acacgacgcc gctggcgccg gtgacctgca tgcgcgcgca ttgtagatca
     3241 tggtgtgtgg ttcgctcagg ctggcgctac agtcctgcgg actcgaacgg cacctccgcc
     3301 ggcagcccga cgtactgctc cgcgaggctc gtcatcgcgg cgcgggagcc ggtgaggtaa
     3361 tcgaggtcgg cgagctgacg ctgatggtcg aacgcgttct cgtccggaaa gcgatgcagc
     3421 gttgacgtca tccaccacga gaaccgctgc gcattccaga cgcggcgaac acacacgccc
     3481 gagtagtcgt ccagcccctc ggtgcacccg cgctcgtaga acgccttcat cgccgctgcg
     3541 agcaccagga catcg
//
LOCUS       DBNGSH010000420         1922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3264197, whole genome shotgun sequence.
ACCESSION   DBNGSH010000420 DBNGSH010000000
VERSION     DBNGSH010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3264197"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="AC16CY_09570"
     CDS             complement(<1..1541)
                     /locus_tag="AC16CY_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR3354078.1"
                     /translation="MSGSTNSARPAGAIASLVAAMRSPRAAAVTLQMFASGLPLGLVW
                     YSIPDWMRDNGVDIRLVGLITLSQAPWTFKVMWAPLMDRYVPPFWGRRRGWMAITQIA
                     LFVLGLLLAGLGQRPEAIWVVGALATGVALASATQDIAIDAYRVEVLHKDEQGPVTGA
                     GVAMYRAAMFVSGGLSISLAARIGWPAVNVLLAFVYLTVLVVTWKSPEPDVQAPPPRS
                     LRDAVWQPFLSFLTRPRALEILSFVVLYKIADQLTQALTRPFLIDMGYGPDERGVALG
                     AVAIAATVVGAFIGGWITTIVGLGHSLWIFGFLQVFSNVGYYLLSILGRPIALAMYGA
                     TGFELLTSGLGTGAFSVLLLRLTERRFSATQYALFSSLFALPRVLAGPVTGFAVSAVG
                     WSTFFLTTMVIGIPGLLMLNRFVPLGVREPDFEREAAPAAPATAAGFLRPAVTSGVAL
                     AVAGALLLSTLDAMDSMRAAPGQGFDFGAALWRLLRPAAIGGWVTILGLLSFAAVGGV
                     FIAAMR"
     gene            1684..>1922
                     /locus_tag="AC16CY_09575"
     CDS             1684..>1922
                     /locus_tag="AC16CY_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3354079.1"
                     /translation="MQLNEYVRRGVVILEPCESLTVENEDEFKQAVRRHLDTGCVRLV
                     LDLAHVTYIDSCGLGTIAQTHLAASRLGGWLKLHNV"
BASE COUNT          378 a          630 c          666 g          248 t
ORIGIN      
        1 cgcatggccg cgatgaagac gccaccgacg gcggcgaagg agagcaggcc caggatcgtc
       61 acccacccac cgatcgccgc cgggcgcagg agccgccaga gcgcggcgcc aaagtcgaat
      121 ccctgcccgg gcgcggcccg catggaatcc attgcgtcga gcgttgacag cagcaatgcg
      181 cccgccaccg ccaacgccac cccggatgtg acggccgggc gcagaaagcc ggccgcggtt
      241 gccggcgccg ccggcgcggc ctcgcgctcg aagtcgggct cgcgcacgcc gaggggaacg
      301 aaccggttca acatcagcag gcccggtatg ccgatcacca tcgtcgtcag gaagaacgtg
      361 gaccagccga cggcgctgac ggcgaacccg gtgaccggac cagccagtac gcgcggcagc
      421 gcgaacaggc tcgagaagag cgcgtattgc gtggccgaga agcgcctttc ggtgagccgc
      481 agcagaagga ccgagaacgc gccggtgcca aggccggacg tgagcagctc gaagccggtc
      541 gcgccgtaca tcgccagcgc gatcggtctg ccgagaatcg acagcaggta gtagccgaca
      601 ttcgagaaca cctgcagaaa gccgaagatc cagagcgagt gacccagacc gacgatcgtc
      661 gtgatccacc cgccgatgaa ggcgccgacg acggttgccg caatagcaac ggcgccgagc
      721 gcgacgccgc gctcgtccgg gccgtagccc atgtcgatca ggaacgggcg cgtcagcgcc
      781 tgggtgagct ggtcggcgat cttgtagagc acgacgaacg agaggatctc gagggcgcgc
      841 gggcgcgtga gaaagctgag gaacggctgc cagaccgcgt cgcgcaggct tcggggcggc
      901 ggcgcctgca cgtcgggctc gggcgatttc caggtgacga cgagcaccgt caggtagaca
      961 aaggccagca gcacgttcac ggcaggccag ccgatgcggg cagcaaggct gatcgacagc
     1021 ccgcctgaca cgaacatcgc cgcgcgatac atggccaccc ctgcgccggt caccggacct
     1081 tgctcgtcct tgtgcagcac ctccacccta tacgcgtcga tggcgatgtc ctgcgtggcc
     1141 gaggcgaggg ccacgcccgt ggccagtgcg ccgaccaccc agatcgcctc cggccgctgg
     1201 ccgaggccgg ccaggaggag accgaggacg aagagcgcga tctgggtgat cgccatccac
     1261 ccgcggcgac ggccccagaa cggcgggaca taacggtcca tgagcggcgc ccacatcacc
     1321 ttgaacgtcc agggggcctg cgaaagcgtg atgagaccga cgaggcggat atcgacgccg
     1381 ttgtcgcgca tccagtcggg aatggagtac cagacgaggc cgagcggaag gcctgacgcg
     1441 aacatttgca gcgtcacggc cgccgcgcgg ggcgaccgca tcgccgccac gaggctcgcg
     1501 attgcgccgg ctggccgtgc tgagttcgtc gagccgctca tcgtttgctg actataaacc
     1561 ggcttctcgg gaggaagggc tgccttcaac accgacgcaa ggggtgtggc gctatttagt
     1621 caaccccagg cgttcgttcg gggcccgact ttcgttagta gagggtaggg gcggaatcga
     1681 accatgcagc tcaacgaata cgtcaggagg ggtgtcgtca ttctcgagcc gtgcgagagc
     1741 ctgacggtcg agaacgagga cgaattcaag caggcggtgc ggcgtcacct ggataccggc
     1801 tgcgtccgtc tcgtgctgga tctcgcccac gtgacctaca tcgacagctg cggcctgggg
     1861 acgattgccc agacacacct ggcggccagc cgtctcggag ggtggctgaa gctccacaac
     1921 gt
//
LOCUS       DBNGSH010000421         5003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2245742, whole genome shotgun sequence.
ACCESSION   DBNGSH010000421 DBNGSH010000000
VERSION     DBNGSH010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2245742"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="AC16CY_09580"
     CDS             <1..203
                     /locus_tag="AC16CY_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354080.1"
                     /translation="SVNYWRTIHPATTVVPRLPVSMGFPLWYCVIAFLLLFVLLLKLR
                     SRVAEQAARLDALYLALDEQRS"
     gene            200..481
                     /locus_tag="AC16CY_09585"
     CDS             200..481
                     /locus_tag="AC16CY_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354081.1"
                     /translation="MMLLRALVVSLLMSVVSTPPLLAQTKPTQQEEFTPISELPPEEQ
                     LAAAPLLIGAYAFVLAVLFGYLVTVSRRLSAIARDINRLTAEVKTAGRG"
     gene            481..651
                     /locus_tag="AC16CY_09590"
     CDS             481..651
                     /locus_tag="AC16CY_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354082.1"
                     /translation="MPTAAHFIYIPGVLLLGIVIGWILGSRAAADAYAARLRRQDARP
                     DQGPRTKDGPRT"
     gene            complement(691..1425)
                     /locus_tag="AC16CY_09595"
     CDS             complement(691..1425)
                     /locus_tag="AC16CY_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3354083.1"
                     /translation="MKYLVISDIHANLEALEATLASAPSHDATLVLGDLVGYGADPNA
                     VIERVRALPGATIIRGNHDKVGAGIEDVEAFNHLAKQAIAWTASALTPANRTWLAALP
                     AGPINIDPLVQICHGSPFDEDAYIFDDLDARRALLVTERPLCLFGHTHVPAVYLFDND
                     LRHLGPPRGKEFSVPMQGKARYLVNCGAVGQPRDGDPRAAFGVLDTADRTLTVLRVPY
                     DVAGAQSKIVTAGLPEVLAQRLAIGR"
     gene            complement(1430..1927)
                     /locus_tag="AC16CY_09600"
     CDS             complement(1430..1927)
                     /locus_tag="AC16CY_09600"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR3354084.1"
                     /translation="MKADKLYLVGFMGAGKSTVARALGRRTGWRVEDIDERIEAREHR
                     TVAAIFGQQGEAHFRHLERMELGELVSARHVVVATGGGTFVEADNRALMLADGVVAWL
                     DLPLAGVLERVPVDGRRPLAADRAQMEQLFTRRQLAYAHAHVRVDASRPVEEIVGRLL
                     EWIDF"
     gene            complement(1924..3228)
                     /gene="aroA"
                     /locus_tag="AC16CY_09605"
     CDS             complement(1924..3228)
                     /gene="aroA"
                     /locus_tag="AC16CY_09605"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR3354085.1"
                     /translation="MPPPRTVTVHPTPCVRGQVRPPGDKSISHRYALLSALADGTSTI
                     RGYSTGGDCRSTLNCLRALGVSIEPVDQTPAGLTLEIAGLGLGGLRPPGAALDAGNSG
                     STMRMLAGVLAAHPFSATIEGDESLRRRPMKRVAAPLERMGARIESNDGWPPLTIHGT
                     ASPRPIDFEPDVPSAQVKSAVLLAGLHADGRTTVTERTGTRDHTERALVEFGVAVTRE
                     GNKVAIQGRQRLTARALEVPGDLSSAAFWMVAAASLSGSEIVIRQVGLNPTRTGILEI
                     LQRMGAAVVVQADSDDGGEPVGSVTVRHGALGSAEVAPHEVPGVIDELPVLGALATHG
                     GEIRVTGAGELRVKESDRISALADGFRRMGGEIEEFPDGFHVRGHRRLRGGDVDARGD
                     HRLAMAFAIAALGASGPTHIHDAGAAAVSYPEFFTVLESLRA"
     gene            3350..3826
                     /locus_tag="AC16CY_09610"
     CDS             3350..3826
                     /locus_tag="AC16CY_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3354086.1"
                     /translation="MSRAKTVCLEFHSSYEILDFVQVASDHLGRLAGLDDEALHWVGV
                     AVRESVINAIKHGNAGDEGKYVRVEFTPLNSHTPSGIAIRVRDEGAGFDPDKIPDPLA
                     PENMLKSSGRGIFIIRNFMDEMVLQRAPEGGMEVVMVKRVTATQTDSDSRSSLPGE"
     gene            3823..4656
                     /locus_tag="AC16CY_09615"
     CDS             3823..4656
                     /locus_tag="AC16CY_09615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3354087.1"
                     /translation="MSAPDPVLLATAAEIVLRAGDIQMSRRQSGFHVEKKGTIDLVTE
                     VDLECERMCRSVLAERFPDHDVLAEELSSGPAEPASSRYRWVFDPLDGTTNYAHGLPI
                     FCASLALEIDGEAEVGAIYDPTRKELFTAERGEGAYVNGTRLHVSDTAGLGDSLLVTG
                     FPYDVHERVHDLVALFGAYLGRARAVRRLGSAALDLCYIAAGRFDGFWEQRLKPWDVA
                     AGGLIVTEAGGRVTGMDGTRFDPAAGHLVASNGRIHDEMLGVIATHQGAGGKGRGAGE
                     R"
     gene            4663..>5003
                     /locus_tag="AC16CY_09620"
     CDS             4663..>5003
                     /locus_tag="AC16CY_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR3354088.1"
                     /translation="MRAFIDALYAFALAIGGPGLFVVTFLDSSFVPLPQINDLLVVLM
                     VIGHKSWVVYYAVMATAGSVAGCYVIYYLADKGGAAFVRKRLKPGHLERGLKAYQRYG
                     VLALVVPALLPP"
BASE COUNT          810 a         1690 c         1668 g          835 t
ORIGIN      
        1 tctccgtcaa ctactggaga acgattcacc cggcgacgac ggtcgtgccc aggctgccgg
       61 tgtcgatggg gtttccgctc tggtactgcg tcattgcatt cctgctgctg ttcgtgctgc
      121 tcctcaaact ccgctcgcgc gtcgccgagc aggccgcgcg gctcgacgcg ctgtatctcg
      181 ctctggacga gcaacgctca tgatgctgct gcgtgcgctg gttgtcagtc tcctgatgtc
      241 ggtcgtgtcc acgccgccgc ttctggcaca gacgaagccg acacaacagg aggagttcac
      301 tcccatcagc gagcttccac cggaggagca gctggcggcc gcgccgctgc tcatcggcgc
      361 gtatgcattc gtgctcgcgg tgctcttcgg ctacctggtg accgtctcac gccgattgag
      421 cgctatcgcg cgcgacatca accggctcac cgctgaagtg aaaacagccg gcaggggctg
      481 atgccgaccg ccgcccactt catctacatt ccgggagtcc tgctcctcgg gatcgtgatc
      541 gggtggatcc tgggctcgcg tgccgccgcc gacgcgtacg cggccagact cagacgccag
      601 gacgcgcgcc cggaccaagg accaagaacc aaggacggac caaggaccta gaacggacca
      661 agaaccaagc acgaagaacc aaggaccatg ctagcgtcca atcgcgagac gctgagcgag
      721 gacttccggc agtcccgcag tcacgatctt ggactgggcg ccggccacgt cgtagggcac
      781 acgcaatacc gtgagcgtcc ggtcggcagt atcgagcact ccgaaggctg cacgcgggtc
      841 gccgtcgcgc ggctgaccga ccgcgccgca gttgaccagg tacctggcct tcccctgcat
      901 cggcacgctg aactccttgc cccggggagg gccgaggtgc cggagatcgt tgtcgaagag
      961 gtacacggcg ggcacgtgcg tgtgcccgaa caggcagagc ggacgctcgg tcacgagaag
     1021 agcccgcctg gcatcgaggt cgtcgaagat gtaggcatcc tcgtcgaacg gcgagccgtg
     1081 gcagatctgc acgagcggat cgatgttgat cgggccggcc ggcagcgcgg cgagccacgt
     1141 cctgttcgcg ggcgtcagtg cggaggcggt ccacgcgatt gcctgcttcg ccaggtggtt
     1201 gaacgcctcg acgtcttcga tgccggcccc caccttgtcg tggttgcctc gaatgattgt
     1261 ggccccggga agggcacgga cgcgttcgat gaccgcgttc gggtccgcgc cgtagccgac
     1321 gagatcgccg agcacgagcg tggcatcgtg gctcggagcg ctggcgagcg tcgcctcgag
     1381 cgcctcgagg tttgcgtgta tgtcgctgat gaccaggtat ttcatgccgt cagaagtcga
     1441 tccattcgag cagtcgcccc acgatttctt cgaccggccg gctcgcgtcc acccgcacgt
     1501 gggcgtgcgc gtaggccagc tgccggcgtg taaacagttg ttccatctgc gcacgatccg
     1561 ccgcgagcgg acgtcgtccg tcgaccggca cgcgctcgag cacgccagcc agcgggaggt
     1621 ccagccacgc caccacaccg tcggcgagca tcagcgcgcg gttgtccgct tcgacgaagg
     1681 tgccgcctcc ggtcgccacc acgacgtgtc tggcggagac aagctcgccg agctccatcc
     1741 gctccaggtg tcgaaaatgc gcctcgcctt gctggccaaa gatcgcagcc accgtccggt
     1801 gctcgcgcgc ctcgatgcgc tcgtcgatgt cttccacacg ccagccggta cgccggccga
     1861 gagcgcgggc caccgttgac ttcccagccc ccatgaagcc gacgagatag agcttgtccg
     1921 ccttcacgcg cgaagtgact cgagcacggt gaagaactcg gggtaggaca cggcggccgc
     1981 cccggcatcg tggatgtggg taggcccgga cgcgcccagc gccgcgatgg caaaggccat
     2041 cgccagccga tgatcgcctc gcgcgtcgac gtcgccgccg cgcaggcgcc gatgcccgcg
     2101 cacgtggaac ccatcaggaa actcctcgat ctcgcccccc atgcgtcgaa aaccgtccgc
     2161 gagggcagag atccgatcgc tctctttcac tctcagttcg ccggctcccg tcacgcggat
     2221 ctcgccgccg tgcgtggcca gcgcgcccag cacgggcagt tcgtcgatga ccccgggaac
     2281 ctcgtgcggg gcgacctcgg cggagccgag ggccccatgc ctgaccgtga cgctccccac
     2341 gggctcaccg ccgtcgtcgc tgtcagcctg gaccacgacc gcggcgccca tccgctgcag
     2401 gatttccaat atccccgtgc gtgtgggatt gagccctact tgccttataa cgatctcgga
     2461 ccctgaaagc gacgcggccg cgaccatcca gaacgccgcc gaggacaggt cccccgggac
     2521 ctcgagtgcc cgggcggtca gacgctgcct cccctggatg gccactttgt tgccctccct
     2581 cgtcacggcc accccgaact cgaccagcgc gcgctccgta tgatcccgcg tgcccgtccg
     2641 ctcggtgacg gtcgttcgcc cgtcggcgtg aagaccggcc agcagcacgg cgctcttgac
     2701 ctgcgcgctg ggcacatcgg gctcgaagtc gatcgggcga ggggacgcag tgccgtgaat
     2761 cgtcaacgga ggccagccgt cgttcgactc gattcgtgcc cccatgcgct cgagcggtgc
     2821 cgcgacgcgc ttcatggggc ggcgcctcag cgactcgtcg ccttcgatcg tcgcggaaaa
     2881 agggtgcgcc gcgagcacgc cggccagcat acgcatcgtg ctgccggaat tgccggcgtc
     2941 gagcgcggcg cccggcgggc gaaggcctcc gaggccgaga cccgcgatct cgagggtcag
     3001 gccggcgggg gtctgatcga ccggctcgat cgacacgccg agcgcgcgaa gacagttcag
     3061 tgtcgaccgg cagtcgccgc cggtggagta gccccggatc gtggacgttc catcggcgag
     3121 ggccgataac agggcgtaac gatgcgagat tgacttgtct cccggagggc ggacctgtcc
     3181 ccgtacacac ggtgtgggat ggacagtgac ggtccgcggc ggcggcatgc agttagtcta
     3241 gcttgtcgct ggtggccggt cgccggtggc tggttccggg ttcacgattt tcgctaccgg
     3301 cgaccagcta tcgggtacga gctatggcta taatttcctg ttagggttca tgagccgcgc
     3361 caaaaccgtc tgtctcgagt tccacagcag ctacgagata ctcgacttcg tgcaggtggc
     3421 gagcgatcat ctcggccggc tggccgggct cgacgacgag gcgctccatt gggtgggcgt
     3481 ggccgtccgg gagtcggtca tcaacgcgat caagcacggc aacgccggcg acgagggaaa
     3541 gtacgtgcga gtcgagttca cgccgctcaa ctctcacacg ccatccggca ttgccatccg
     3601 cgtccgcgat gaaggggccg ggttcgatcc cgacaagatt cccgatccac tggcgccaga
     3661 gaacatgctg aagtccagcg gccgcggcat tttcatcatc cgcaacttca tggacgagat
     3721 ggtgctgcag cgggcgcccg agggcggcat ggaagtggtc atggtcaagc gcgtgacggc
     3781 gacgcaaacc gactctgact cccgatccag tctgcccggc gagtgagcgc ccccgatccg
     3841 gtactgctcg ccacggcagc cgagatcgtc cttcgcgccg gcgacatcca gatgtcgcgc
     3901 cggcagtccg ggttccacgt cgagaagaaa ggaacgatcg atctcgtcac cgaggtcgac
     3961 ctggagtgcg aacgcatgtg ccgcagcgtg cttgcggaac gctttcccga tcacgacgtg
     4021 ctggccgagg agttgagcag cggaccggcg gagccggcgt cctcgcgcta ccgatgggtg
     4081 ttcgatccgc tcgacggcac gacgaactat gcgcacggcc tgccgatctt ctgcgcctcg
     4141 ctcgccctcg agatcgacgg cgaggccgag gtgggcgcga tctacgaccc cacgcggaag
     4201 gagctgttca ccgccgagcg cggcgagggc gcctatgtga acgggacgcg ccttcatgtc
     4261 tccgacacgg ccggactcgg ggactcgctg ctcgtgaccg ggtttcccta cgacgtccac
     4321 gagcgggtgc acgacctggt cgcactgttc ggcgcctacc tgggccgtgc gcgggcggtg
     4381 cgccgcctcg gttcggccgc tctcgacctc tgctacatcg cggcagggcg cttcgacggg
     4441 ttctgggaac aacgcctcaa gccctgggac gtcgctgccg gggggctgat cgtgaccgaa
     4501 gcgggcggcc gcgtgaccgg gatggacggc acgcgcttcg atccggcggc cggccacctc
     4561 gtggcgtcca acgggcgcat tcacgacgag atgctcgggg tgatcgcgac acatcagggg
     4621 gcagggggca aggggcgggg ggcaggggaa aggtgacgta gagtgcgcgc cttcatcgac
     4681 gcgctctacg cgtttgccct cgcgattggc ggcccaggtc ttttcgtggt cacgttcctg
     4741 gattcgtcat tcgtcccgct gccgcagatc aacgaccttc tcgtcgtgct gatggtcatc
     4801 ggccacaagt cgtgggtcgt gtactacgca gtgatggcca cggccggctc ggttgccggc
     4861 tgctatgtga tctactacct ggccgacaaa ggcggcgcgg cgttcgtccg taaacgcctc
     4921 aagccggggc acctcgagcg aggcctgaag gcgtaccaac gctacggcgt gctcgcgctc
     4981 gtcgtgccgg cgctgctgcc gcc
//
LOCUS       DBNGSH010000422         1577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1057482, whole genome shotgun sequence.
ACCESSION   DBNGSH010000422 DBNGSH010000000
VERSION     DBNGSH010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1057482"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="AC16CY_09625"
     CDS             <1..522
                     /locus_tag="AC16CY_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354089.1"
                     /translation="PDARTSRWPPLLRWLDGLGLEMLERLVLRSPRLQRFVLESHRRA
                     LATLVSRLPRIDRVAIVGGGLFPRTALILRDLLPGARLVVIDADENNLETARSLIGGA
                     AEFVRRRYRATEEAPGFDLVVIPLSLDEGRGAIYAHPPARAVLVHDWIWRVRGESAIV
                     SPLLLKRLNLVRA"
     gene            519..>1577
                     /locus_tag="AC16CY_09630"
     CDS             519..>1577
                     /locus_tag="AC16CY_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3354090.1"
                     /translation="MKAASLLAVLIVAKLAMLNGRAIPLSIWDPIAYFWQDVLVALVF
                     GLAGFLSYVVSGFSRTVRSRLETAIYWIVVLYAAANIPVGRALSTPLTWPMVRAAGGP
                     LADSILQYLTWSNVLLVALTIAAAAVLPRVPPLRGRASQVAVACALAVVALGPFAGSR
                     VDASGLDRNILVALVRSALPQIRSRPSDDDWRASRFDTDHIDDLSPLTGLAAGRNVVV
                     VVLESTAAQYLAPYGGEADVMPSLTALSEHALVFDNAYAVYPESIKGLFSILCSTSPA
                     FDVEAEAYAGVACNPLPGVLRQAGYRTAMFHSGRFAYLGMEAVIGNRGFDVLEDAGHI
                     GGNHESSFGVDEPSTVRRM"
BASE COUNT          205 a          540 c          526 g          306 t
ORIGIN      
        1 cccgatgcgc gcacgagccg atggccgccg cttctcaggt ggctcgacgg cctcggcctc
       61 gagatgctcg aacggctcgt cctccgctcg cctcgcctgc agcgcttcgt tctcgagagc
      121 catcggcgcg cgctcgcgac actcgtgtcg cggctgccgc ggatcgatcg cgtggcgatc
      181 gtcggcggag ggctctttcc gcgaacggcg ctcatccttc gcgacctgct gcccggcgca
      241 cgcctggtcg tcatcgatgc cgacgagaac aacctcgaga cggcacgttc gctgattggc
      301 ggcgccgcgg agttcgttcg tcggcggtat cgtgcaacgg aagaagcgcc aggcttcgat
      361 ctcgtcgtca ttccactctc gctggacgaa gggcgcggcg ccatctacgc gcatccgccg
      421 gcaagagcgg ttctcgtcca tgactggatc tggcgggtgc gcggcgagag cgccatcgtg
      481 tcgcctctgc tgctcaagcg cctgaacctg gtgcgcgcat gaaagcggcc agtctcctgg
      541 ccgtcctcat agtcgccaag ctcgcgatgc tgaacggccg ggcgattccg ctttcgatat
      601 gggacccgat cgcgtacttc tggcaggacg tcctcgtcgc cctcgtgttc ggacttgccg
      661 ggttcctttc gtacgtagtg tccggcttta gccggacggt tcgttctagg ctcgaaacgg
      721 cgatctactg gatcgtcgtg ctgtacgcgg cggccaacat cccggtgggg cgcgccctgt
      781 ccacgccgct gacgtggccg atggtgcgtg ccgcgggtgg cccgctcgcc gactcgattc
      841 tgcagtatct gacgtggtcg aacgtgctcc tcgtggcgct gacgatcgcc gctgcggccg
      901 tccttccacg ggtgcccccc ctgcgcggcc gcgcgtcgca ggtggccgtc gcgtgcgcgc
      961 tggcggtcgt ggctctggga ccgttcgcgg gcagccgcgt tgacgcgtct ggcctcgacc
     1021 ggaacatcct cgtggccctc gttcgaagcg ccctgccgca gatccgctca cggccgtctg
     1081 acgacgactg gcgggcgtct cgcttcgaca ccgatcacat cgacgatctg tccccattga
     1141 cgggactagc cgccggtcga aacgtcgttg tcgtcgtgct cgagtccacg gcggcgcagt
     1201 atctcgcgcc gtacggtggg gaagccgacg tgatgccgtc gctcacggcg ctgtccgagc
     1261 acgcgctggt gttcgacaac gcgtatgccg tctatcccga gagcatcaag gggctcttct
     1321 cgatcctctg ctcgacgtcg cccgctttcg acgtcgaagc cgaggcgtac gccggcgtcg
     1381 cgtgcaaccc gctgccgggt gtgctccggc aggcgggcta tcgcacggcg atgtttcatt
     1441 ccggccggtt cgcctatctg ggcatggagg ccgtcatcgg caatcgcgga ttcgacgtcc
     1501 tcgaggacgc gggccacatc ggaggcaatc acgagtcgag cttcggcgtg gacgagccgt
     1561 ccacggttcg gcggatg
//
LOCUS       DBNGSH010000423         5071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4283565, whole genome shotgun sequence.
ACCESSION   DBNGSH010000423 DBNGSH010000000
VERSION     DBNGSH010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4283565"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="AC16CY_09635"
     CDS             <1..1434
                     /locus_tag="AC16CY_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3354091.1"
                     /translation="DAANDYGITPLLAASRTGDAAMVDLLLKSGANPSLAHPEGETPL
                     MAASRSGSVPAVRLLLARGVQVNTAESFQKTTALMWAAAEGHVDVVDVLIEAGADPNL
                     QGHVTSLTKRHNADHPTGGFTALMYAARSGNEALVRRLVARGANVNLKNGDGASAAMV
                     AMYNDRFDLAATLIELGSDANDGSLYVAVEMRDATTDQFAFDGSRRRPDHVNTLTALD
                     LIRILLERGADPNKNFAGQFHSTSMPNTDRFDNTPLFRAAIAADVEALKLLAARGADL
                     EQTPAVVAAAKGDTDETPPAEGRGRGNPNAGRTVAMVAMSGGRGPAMTGGPAYIRDGP
                     APYREPGSRKPEDAFAALLAAGANPNAAGPDGAPLLHQAVRMRNLEMIRALAGAKVDF
                     TQKNKEGFTALDVAEGKQQPAGRAGGRGGPPPGGGRGRGGRGASQQDVAKLLRELMGL
                     PPAEPSTAPDSAAPDSAPDSPDAGDAN"
     gene            1440..3914
                     /locus_tag="AC16CY_09640"
     CDS             1440..3914
                     /locus_tag="AC16CY_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3354092.1"
                     /translation="MLFAAAIAFLAATVSATTQELAAQPSAKAAAPVAYQAMVGKYCV
                     GCHNTRNPLPAGAPLALDTASLVDPGADAATWERVVKKLGVGAMPPQGSPTPGREELA
                     RFRSSLAASLDAAAARRSSPGRFVLHRLNRTEYANAVRDLVGVTVDVTDLLPSDGGDF
                     GFDNIATALTTSPLLLERYLSAGLRISELAVGDAAAEPGTATYTISTVVTQNQHVDGL
                     PLGTRGGILVRHPFPADGDYVFSGRLLKTVAEGLAGVEGHETPHLFIVTLDGKQVFSS
                     PIGGKNDHDRATENKPVAREEFDRRMMSPRIKVTAGLHEVGFTFVERPGQEQNMWQPS
                     LRATQEAHNPSGMPRLRNAIIEGPYGVSGVSSTESRQRLFVCTPRSASEEPGCAEKIL
                     STVARRAFRRPVTKADVEAPLSLYRDERSAGGDFNAGIRAGLARILTSPAFLFRSESD
                     PAGLPAGAAHPITDLELASRLSFFLWSSIPDDELLDAAIDGKLRKRGVLDAQVRRMIA
                     DTRADALMSAFTGQWLQLRNLDKVTPDVLLFPDFDDNVRQALRRETELFFGSIVRENR
                     SALDLLQADYTFVNERLARHYGIGGVYGSRFRRVPVADPNRRGLLGHGSILAMTAVAT
                     RTSPVLRGKYIISNLLNTPPLPPPAVVPDLEESAHKDKPSTVREQLERHRANPVCGSC
                     HRNIDPVGFALENFDAVGQWRTTTREGLAIDAAGVLADGTKVDGPQALRQALLSRPDV
                     FAGTITEKLLIYALGRGLEPVDMPVVRSVVRNAAVQNYAMQAIVLGIVKSDPFQMRTK
                     LSDTVGAPTATLAAARPAEGGPRDKE"
     gene            3918..>5071
                     /locus_tag="AC16CY_09645"
     CDS             3918..>5071
                     /locus_tag="AC16CY_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3354093.1"
                     /translation="MIVTKKHLPRRTFLRGAMGAVVAMPFLDAMIPALTAQAKRPFRF
                     GAIYVPNGIYPQLWHPETTGSTFELKQVMQPLEAYRSHLVTISGMKAPDGNPDMGGVH
                     MGASAAWLNGTGPVTVMAKFVTQSKKSIDQYIADAVAGDTPLRSLEVGTEDMGTSAGA
                     CDGYPCVFFNTISWRDDKSPLPVAVNPRVTFERIFGEAGSTGRRRANMRRKQSLLDSV
                     AAEAGTMRRTLGPADGAILDEYLTNIRDVEQQLTRMDARAAATPEGAVAPLGIPDNVD
                     DHLTVTYNLMLLAFQADISRVFTFMVGHEGSGRSYAHIGIPEPHHPVSHHGDTPEGIA
                     KYAKLTTYHVAKLAEFIGKLQETADGDGTLLDRSLIYFGSGMGNGNAHDR"
BASE COUNT          852 a         1751 c         1757 g          711 t
ORIGIN      
        1 gacgccgcaa acgactacgg catcacgccg ctgctcgctg ccagccgcac gggcgacgcg
       61 gcgatggtcg atctcctcct gaagtcgggc gccaatccgt cgctcgcgca tcccgaaggc
      121 gagacgccgc tgatggccgc gtcgcggtcg ggcagcgttc ccgcagtgcg cctgctgctg
      181 gcccgcggcg tccaggtgaa tacggcggag tcgttccaga agaccacggc cctgatgtgg
      241 gcggcggcgg aaggccatgt cgacgtggtt gacgtcctga tcgaggcggg cgcggatccc
      301 aacctccagg ggcacgtcac ctcgctgacc aagcgccaca acgccgatca tccgacgggc
      361 ggcttcaccg cgctgatgta tgccgcgcgg agcgggaacg aggcgctggt gcgccggctg
      421 gtcgcccgcg gtgcgaacgt caatctgaag aacggcgacg gcgcgtcggc ggccatggtc
      481 gcaatgtaca acgacaggtt cgacctggcc gccacgctga tcgagctcgg ctcggacgcc
      541 aacgacgggt cgctctacgt cgccgtggag atgcgcgatg cgacgacgga tcagttcgca
      601 ttcgacggat cgcggcggcg gcccgatcac gtgaacacgc tgacggcgct cgacctgatt
      661 cgcatcctcc tcgagcgcgg ggccgatccc aacaagaact tcgccggcca gttccactcg
      721 acgtcgatgc ccaataccga tcggttcgac aacacgccgc tcttccgcgc ggcgatcgcg
      781 gccgacgtcg aggcgttgaa gctactggcg gcgcgcggcg cggacctcga gcagacgccg
      841 gcagtcgtcg cggcggcgaa aggcgacaca gacgagacgc cgcccgccga ggggcgagga
      901 cggggcaatc ccaacgctgg caggaccgtg gccatggtgg cgatgagtgg agggcgcggg
      961 cccgcgatga cgggagggcc ggcctacatc cgtgacggcc cggcgcccta ccgcgagccg
     1021 ggaagccgca agcccgaaga cgccttcgcc gcgctgctcg ccgcgggagc gaacccgaac
     1081 gcggcgggtc ccgatggtgc tccgctgctg catcaggccg tgcgcatgcg caacctggag
     1141 atgatccgcg cgctcgcggg cgccaaggtc gatttcacac agaagaacaa ggagggtttt
     1201 accgccctcg atgtcgccga aggcaagcag cagcccgccg gaagggcagg cgggcgaggc
     1261 ggtccgcctc cgggaggcgg acgcggtcgc ggcggtcgcg gcgcaagcca gcaggacgtc
     1321 gcgaagctgc tgcgcgagtt gatgggcctt ccgccggccg agccgtcgac ggcgccggac
     1381 tcggcagcgc cggactcggc accggattcc cctgacgcgg gagacgcgaa ttgaggaagg
     1441 tgctgttcgc ggccgcgatc gcgttcctcg cggccaccgt gtcggcgaca acgcaggagt
     1501 tggccgcgca gccgtcggcg aaggcggcgg cgcctgtcgc gtatcaagcg atggtgggca
     1561 agtactgcgt cggctgccac aacacgcgca acccgcttcc ggccggagcg cctctcgcgc
     1621 tcgacacggc cagcctcgtc gatccaggcg ctgacgcagc cacgtgggag cgcgtcgtga
     1681 agaagctcgg cgtgggcgcg atgcctccgc agggatcgcc gacgcccggg cgagaggagc
     1741 tggccaggtt ccggtcgtca ctcgcggcca gcctcgacgc ggccgccgcg cggcggagca
     1801 gcccgggccg gttcgtcctc caccgcttga atcgcaccga gtacgccaac gcggtccgcg
     1861 acctggtggg cgtcaccgtg gacgtcacgg atctgctgcc gagcgacggc ggggacttcg
     1921 ggttcgacaa catcgcgacc gcgctcacga cgtcaccctt gctgctcgag cgctatctca
     1981 gcgcgggcct tcggatctcc gagctcgccg tcggcgatgc cgccgccgag ccgggaacgg
     2041 caacctacac gatcagcacc gtcgtgacgc agaaccagca cgtggacggg ctgccgcttg
     2101 gcacgcgcgg cggcatcctg gtccggcatc cgttcccagc cgacggcgac tacgtcttct
     2161 cgggacgcct gctcaagacg gtggccgaag ggctcgccgg cgtcgaaggg cacgagacgc
     2221 cgcatctgtt catcgtgacg ctggacggca agcaggtctt ctcgtcgccg atcggcggca
     2281 agaacgatca cgaccgcgcc accgagaaca agccagtggc gcgcgaagag ttcgacaggc
     2341 ggatgatgtc gccccgcatc aaggtcaccg ccggccttca cgaggtggga ttcaccttcg
     2401 tcgagcgccc ggggcaggaa cagaacatgt ggcagccgtc gctgcgcgcg acgcaggagg
     2461 cgcacaaccc gtcggggatg ccgagacttc gcaatgccat catcgagggg ccgtacggcg
     2521 tgtccggcgt gagcagcacg gagtcgcggc agcgcctgtt cgtctgcacg ccgcgatcgg
     2581 cctccgagga accgggctgc gccgagaaga tcctctccac ggtggcgcgc cgggcgttcc
     2641 ggcggcctgt cacgaaggcc gacgtggagg cgccgctgtc gctctatcgc gacgaacgca
     2701 gcgccggcgg tgacttcaac gcgggcatcc gcgcgggcct cgcgaggatt ctgacgagtc
     2761 cggcgttcct gttccgctcc gagtcggatc cggctggcct tcccgcgggg gcggcgcatc
     2821 ccattaccga cctcgagctg gcgagccggc tgtccttctt cctgtggagc agcattccgg
     2881 acgatgaact cctggacgcg gcgatcgacg gcaagctgcg caagcgcggc gtgctggacg
     2941 cgcaggtccg ccggatgatc gccgatactc gtgccgacgc cctgatgtcg gcgttcaccg
     3001 gccagtggct gcagctgcgc aacctggaca aggtcacccc ggacgtgctg ctgttccccg
     3061 acttcgacga caacgtccgg caggcgctcc gccgcgagac cgagctcttc ttcgggagca
     3121 tcgtccgcga gaaccgcagc gcgctcgatc tgctccaggc cgactacacg ttcgtcaacg
     3181 aacggctggc gcgccattac ggcatcggcg gcgtctacgg ctcgcgattc cgccgcgtgc
     3241 ccgtggccga tccgaaccgg cgcggcctgc tgggccacgg cagcattctc gcgatgacgg
     3301 cggtggccac gcgcacctca cccgtcctgc gcgggaagta catcatctcg aacctgctga
     3361 acacgccgcc gctcccgccg ccggcggtcg tgcccgatct cgaggagagc gcccacaagg
     3421 acaagccgtc caccgtccgc gagcagctcg agcggcatcg cgcgaacccc gtctgcggtt
     3481 cgtgccatcg caacatcgac ccggtcggct tcgcgctgga gaatttcgac gccgtggggc
     3541 agtggcggac gacgaccagg gaggggctgg ccatcgacgc ggccggcgtg ctggccgatg
     3601 gcaccaaggt cgacgggccg caggcgctgc gccaggcgct gctctccagg ccggacgtgt
     3661 ttgccgggac gatcaccgag aagctgctga tttacgcctt gggccggggg ctggagccgg
     3721 tggacatgcc ggtcgtccgg agcgtggtca ggaacgcggc cgtgcagaac tacgccatgc
     3781 aggccattgt gctgggaatt gtgaagagcg acccattcca aatgcgcacc aagttgtctg
     3841 atactgtagg ggcgccgaca gcgaccctgg ccgccgcccg gcccgccgaa ggcgggccaa
     3901 gggataagga gtagcggatg atcgttacca agaagcacct gcctcgacgg acgttcctcc
     3961 gtggcgccat gggcgccgtg gtcgcgatgc cgtttctgga tgcgatgatt ccggcgctca
     4021 ccgcgcaggc gaagcggccg ttccggttcg gcgcgatcta cgttcccaac ggcatctacc
     4081 cgcagctctg gcatccggag acgaccggca gcaccttcga gttgaagcag gtcatgcagc
     4141 cgctcgaggc gtaccgcagc cacctggtga cgatcagcgg gatgaaggcg cccgacggca
     4201 acccggacat gggcggcgtg cacatgggcg ccagcgcagc gtggctcaac ggcaccggac
     4261 cggtcacggt catggcgaaa ttcgtgaccc agtcgaaaaa gagcatcgat caatacatcg
     4321 cggacgcggt cgccggggac acgccactgc gatcactcga ggtcggcacg gaagacatgg
     4381 ggacctccgc cggcgcgtgc gacgggtacc cgtgcgtgtt cttcaacacc atctcgtggc
     4441 gcgacgacaa gagcccgctg cccgtggctg tcaacccgcg cgtgaccttt gagcgcattt
     4501 tcggcgaagc cggttcgacc ggccggcgcc gcgcgaacat gcggcggaag cagagcctgc
     4561 tcgactccgt cgccgccgaa gcgggcacca tgcgccgcac gctcggcccc gctgacggcg
     4621 ccatcctcga cgagtacctc acgaacatcc gggacgtgga gcagcagctc acacgcatgg
     4681 acgcgcgcgc tgccgcgacg cccgaaggcg ccgtggcgcc actcggcatt cccgacaacg
     4741 tggacgatca cctgacggtg acctacaacc tgatgctgct ggccttccag gccgacatct
     4801 cgcgcgtctt caccttcatg gtggggcacg agggcagcgg ccggagctac gcgcacatcg
     4861 gcatcccgga gccgcaccat cccgtatcgc accacggcga cacgcccgag ggcatcgcga
     4921 agtacgccaa gctgacgacg taccacgtcg cgaagctcgc cgagttcatc ggcaagctgc
     4981 aggagacggc cgacggcgac gggacgctgc tggatcgatc gctgatctac ttcggcagcg
     5041 gcatgggcaa cggcaacgcg cacgatcgca a
//
LOCUS       DBNGSH010000424         4852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2585758, whole genome shotgun sequence.
ACCESSION   DBNGSH010000424 DBNGSH010000000
VERSION     DBNGSH010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2585758"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2678)
                     /locus_tag="AC16CY_09650"
     CDS             complement(<1..2678)
                     /locus_tag="AC16CY_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354094.1"
                     /translation="MRRLWFRSWAALRRALDGGARDQELSEELRAFVERDAESKIRSG
                     MTPEEARRAALIELGGAEQVKEHVRDARAGARVEGTVRDIRYAIRSLGRSPGFSSSVI
                     GSLSVGLAATIAAFAFINGAMLRPFPGVRDQDRLVEVGILANTPFGIPRPSRTALADY
                     PDVVRALGEVTSLDGLASFTDSDVAVTLPQPRSLPAAFVSPNYFDVLGVQPEIGRTFA
                     PEEGRAESAVVIIGHSLWTREFGRDPSVVGRPVQVAGQLFEIIGVAPRGFAGTTQNPI
                     QTGAELWLPIALANVVGKDERLIRYVGRMRDGVRVDRVEAELGVVAGRLGIARPADNA
                     PGRVVTSAANPSEPVLVEVSGLSRLGSDDAVVLVALILPVPFLVLVLACVNAANLLLV
                     RASGRGREVAVRLALGASRLRLVRQLVIESFVLAVGAAVLALPLAWAGLQVGAAYMTF
                     PMPLDGTVVAGTLVAAFVTALGFGLAPALQASGQRPSAALGTAHAGSGGTRSESRARR
                     ALVAGQVALSLGLLAVGFQLTAGLESLAEPPGSDPDGLLMASFDLEQLRFSSDESGAF
                     YEALVDGASRIPGVEAAGLADRELLWTWEREGNNSVVVDPRGAAALAASEPVNGLPGV
                     FAFQEGQRFALGGSAGGDLLRAAGLDLLEGREFVAADRRTIPEVAIVTERLAAQIFQG
                     AALGRTLGVRTTSMGAEVRIVGIVESPAELSDPNLAVIFFPSPVHEGIARTLYLRADG
                     PAGRLAPALRDLVARLDPEVPILDIATLDQKIRADTVFERTLARAAAVLGIVALLLAG
                     MGLYGVTSYSVAMRRREIAVRMALGARADRVVTMVLRQGLALAIAGSVLGGVVAVAAG
                     FVIQAEVFGVPGVGFAALGGSAALLAAAMLLASILPA"
     gene            complement(2683..>4852)
                     /locus_tag="AC16CY_09655"
     CDS             complement(2683..>4852)
                     /locus_tag="AC16CY_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3354095.1"
                     /translation="YFDVLGVRPEIGRTFAPGEGGAAAPVAIISRALWMREFGGDPSV
                     IGRPIQAGGRQTLDVIGVAPPGFAGTTGRPAVDLWLPIAFVDRVAVDDPFGRSGTRLI
                     RYVGRMRDGVGVARVEAELGVVARRLGVGRPAVNPGSGRPVNPVSGQLIASADASVER
                     VTVEVSGLSRRDVDELALMVAVILPVPLLVLTLACVNAANLLLVRASGRSREVAVRLA
                     LGASRSRLVRQLVIESLVLALGASVVALPLAWSGVQLIAAFVMLPMPLDGTVVAGALV
                     TAFLTALGFGLVPALHATRRHPSAALGTSPAGSGGTRAEARGRRVLVAGQVALSLGLL
                     AAAFQLTSPLESLAEPPGTDPDRLLMASFDLAQLRFSSGESDAFYEALLDRASRLPGA
                     EGAALSSHDFAAGWISNLVPVDTGDGPRDPLGRPIGLLVSASSAEGDFFKVLGLDLRQ
                     GREFVAADRRDIPEVAIVTEHFAAQIFGGAALGRSLRVSTSLIGEAEAEVRIVGIVES
                     PVERNIDNVRGPSLVKPSASGEVAAIFFPSPLQDGTARTLSVRADGPAGRLAPAIRDI
                     VAQIDPRVPILELATLDQRIPDITQIIRGLARAAAVLGIVALLLASIGLYGVTSYGVA
                     MRRREIAVRMALGAQADRVIAMVLRQALTVATIGAVLGGLIAIAAGVVIQAQLFGVPG
                     VDVATLGGSAGLLAVAMLTASILPAQRAARLDPNLVLREE"
BASE COUNT          896 a         1725 c         1630 g          601 t
ORIGIN      
        1 gcgggcagga tgctggccag gagcatcgcc gctgcaagca gcgccgccga gccgcccagc
       61 gccgcgaacc cgactccggg gacaccgaac acctccgcct ggatgacgaa tccggccgcg
      121 acggcgacca cacctccgag aacggaaccg gcgatcgcga gggccaggcc ctgcctgagg
      181 accatcgtga cgacgcggtc ggcccgtgca cccagcgcca tccgaaccgc gatctcccgc
      241 cgtcgcatcg cgacgctata ggaggtcact ccgtataggc ccatgccggc gagcaaaagc
      301 gcgacgatgc ccagtacggc cgcggccctc gccagcgtgc gttcgaacac agtgtccgcg
      361 cgaatcttct ggtccagagt ggcgatatcc aggattggca cttcggggtc gagccgggcc
      421 accagatcgc ggagggctgg cgcaagcctg ccagccggac catcagcacg gaggtagagc
      481 gtccgtgcga tgccttcgtg gacgggcgag gggaagaaga tgaccgcaag gttcgggtct
      541 gaaagctccg cgggagactc cacgataccc acgatccgga cctcggcccc cattgaagtc
      601 gttcggacgc cgagggtccg gccgagggcc gcaccctgaa agatttgtgc ggccaggcgc
      661 tcggtgacga tcgccacttc cggtatggtg cgtcggtctg cggcgacaaa ttcgcggccc
      721 tcgaggaggt cgagcccagc ggccctgagc aagtccccac cggcagaccc gccgagtgcg
      781 aaccgctgcc cctcctggaa agcaaaaact cctggaagtc cgttgaccgg ctccgaggcc
      841 gccagagcgg ctgctccgcg tgggtccacg acgaccgagt tgtttccctc gcgttcccag
      901 gtccacaaga gttcgcggtc cgcgaggccc gcggcctcga ctcctggaat ccgtgaggca
      961 ccatccacaa gcgcctcata gaacgcgccg ctttcgtcgg atgagaatcg gagttgctcc
     1021 aggtcgaacg acgccatcag cagaccgtcc ggatccgagc cgggcggctc cgccagcgat
     1081 tccagcccgg cggtcaactg aaacccgacg gccagcagac ccagcgagag cgcgacctga
     1141 ccggccacga gtgcccggcg ggcgcgggac tcggaacgcg tgcctccgct tcccgcgtga
     1201 gcggtgccga gcgcagcgga cggacgctgg ccgctggcct gcaatgcggg ggcgagtccg
     1261 aatcccagag ccgtcacgaa cgcggccacg agcgtccctg cgacgacggt cccgtcgagc
     1321 ggcatcggaa acgtcatgta cgcggcgccc acctgcaggc cggcccacgc cagcggcagc
     1381 gcgagaaccg cagcaccgac ggccaggacg aagctttcga tgaccagttg cctgacgagc
     1441 cggagccgtg acgcgccgag cgccaggcgg accgccacct cacggccgcg cccggaggca
     1501 cgcaccagga ggaggttcgc ggcgttgaca caggcgagca cgagaacgag aaagggaacg
     1561 ggcaggatga gggcgaccag cactacggca tcatcgctcc cgagtctcga caggcccgac
     1621 acctcgacca ggaccggctc cgacgggttg gctgcggacg tgacaacccg cccgggggcg
     1681 ttgtcggcag gacgagcgat gccgagccgc ccggcaacga cgccgagctc ggcctcgacg
     1741 cgatcgacgc gaacaccgtc gcgcatgcgg cccacgtatc gaatgaggcg ctcgtccttc
     1801 ccgacaacat tcgcaagagc gatcggcagc cagagctcgg cccccgtctg aattgggttc
     1861 tgagtcgtgc ctgcgaatcc ccgcggcgcg acgccgatga tctcgaataa ctgccctgcc
     1921 acctggaccg gccggccgac gacggacgga tcgcgcccga actctcgcgt ccacagactg
     1981 tgaccgatga tcacgaccgc agactcggcc cggccttcct cgggcgcgaa ggtccgtccg
     2041 atctccggct ggacgccgag cacgtcgaag tagttcggcg acacgaaggc ggccggaagg
     2101 gagcgaggct gcggcagggt caccgccacg tcggagtcgg tgaagctggc cagaccgtcg
     2161 agactggtca cctcaccgag agcgcgaacg acgtccggat agtccgccaa tgcggtccgg
     2221 gagggtctcg ggatgccgaa gggcgtgttc gccagaatcc ctacctccac gaggcgatcc
     2281 tgatcccgga cacccggaaa cgggcggagc atcgcaccgt tgatgaacgc gaatgccgct
     2341 atggtggccg ccaggcccac gctgagactc ccgatcacgg aggacgagaa cccaggtgac
     2401 cggccgagac tgcggatggc gtagcggata tcccggaccg tgccttccac acgggcgccc
     2461 gcccgcgcgt cccggacatg ctccttgacc tgttcggcgc cgcccaactc gatgagggcc
     2521 gcgcgccgtg cctcctccgg ggtcatgccc gaccggatct tcgactcggc gtcgcgctcg
     2581 acgaacgcgc ggagctcctc gctgagctcc tggtccctcg cgccgccgtc cagcgcgcgg
     2641 cgcaacgcgg cccacgagcg aaaccacaga cgtctcatcc gcctactctt ctcgcagcac
     2701 cagattcggg tccagccgcg cggcccgctg ggccggcagg atgctggcgg tgagcatggc
     2761 cacggcaagc agccctgccg atccgcccag cgtcgcgaca tcgactccgg ggacgccgaa
     2821 caactgcgcc tgaatgacga ccccggccgc gatcgcgatg aggcccccga gaaccgcgcc
     2881 gattgtcgca acggtcaggg cctgccgcag gaccatggcg atgacgcggt cggcctgcgc
     2941 gccaagcgcc atccgaacgg cgatctcccg gcgtcgcatc gcgacgccat aggaggtcac
     3001 tccgtagagg ccgatgctgg cgagcaggag cgccacaatg cccaggacgg ccgccgccct
     3061 cgccagcccg cggatgatct gcgtgatgtc gggaatccgc tggtccagag tggccaattc
     3121 caggatgggc actcgaggat cgatctgcgc cacgatgtcg cggatcgccg gtgcaaggcg
     3181 gccggccgga ccgtcggcgc ggacagaaag cgtccgcgcg gtgccatcct gtaggggcga
     3241 gggaaagaag atggccgcga cctcgccgga agcggacggc ttcacgaggc tggggccgcg
     3301 gacgttgtcg atgttgcgct ccacaggcga ttccacgatt cccacgattc ggacctcggc
     3361 ctccgcttcc ccgatcaacg atgtcgacac acggaggctt cggccgagtg ccgcgccgcc
     3421 gaagatctga gccgcaaagt gttcggtgac gatcgccacc tcggggatgt cgcgccggtc
     3481 cgccgcgacg aattcgcggc cttgcctgag gtcgagcccc aggaccttga agaagtcgcc
     3541 ttctgcagac gacgcgctca ccagaaggcc gatcggtcgt cccaacggat cacgcggccc
     3601 gtcgccggta tccactggaa cgaggttgct gatccaaccc gccgcgaaat cgtgactcga
     3661 cagcgcggcg ccctcggctc cgggaagcct cgacgcacgg tcgagaagcg cctcatagaa
     3721 cgcgtcgctc tcgccggatg aaaagcggag ctgcgccaga tcgaacgagg ccatcagcag
     3781 gcggtccgga tccgtgccgg gcggctccgc caaggattcc agcggggacg tcaactggaa
     3841 cgcggctgcc agcaggccca gcgagagcgc gacctgaccg gcgacgagga cccggcggcc
     3901 gcgggcctcg gcacgcgtgc cgccgctccc cgcgggagag gtgccgagcg ccgcggacgg
     3961 gtgtcgacgg gtggcatgaa gcgcggggac gagcccgaat cccagagccg tcaggaacgc
     4021 ggtcacgagt gcgcccgcca cgaccgtccc gtccagcggc atgggaagca tcacaaacgc
     4081 tgcgatcaac tgcacacccg accacgccag cggcagcgcg acgaccgagg caccgagggc
     4141 caggaccaag ctttcgatga ccagttggcg gacgagccgg gaccgggacg cgccgagcgc
     4201 cagacggacc gccacctcac ggctgcggcc ggaggcacgc accaggagga ggttcgcggc
     4261 gttgacacag gcaagcgtca gaacgagaag gggaaccggc aggatgacgg cgaccatcag
     4321 ggccagttcg tccacgtcgc gtctcgacag accagacacc tcgaccgtga cccgctccac
     4381 cgaggcgtcc gcggacgcga tgagttgccc ggacacaggg tttacgggcc gcccggaccc
     4441 agggtttacg gcaggacgac cgacgccgag ccgacgggcc acgacgccga gctcggcctc
     4501 gacgcgggcg acgccgacgc cgtcccgcat tcggcccacg tatcgaatca aacgagtgcc
     4561 ggaccgcccg aacggatcgt ccaccgcaac acgatcgacg aaagcgatcg gcagccacag
     4621 atcgacggct ggccgcccgg tcgtgcctgc aaagcccggt ggcgcgacgc cgatgacgtc
     4681 gagcgtctgc cgcccccccg cctggatcgg ccgtccgatc actgaagggt ctccgccgaa
     4741 ttcccgcatc cacagcgcac gactgataat cgcgaccgga gctgcagccc cgccctcccc
     4801 gggcgcaaag gtgcgtccga tctctggccg gactccgagc acgtcgaagt ag
//
LOCUS       DBNGSH010000425         6678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4453300, whole genome shotgun sequence.
ACCESSION   DBNGSH010000425 DBNGSH010000000
VERSION     DBNGSH010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4453300"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="AC16CY_09660"
     CDS             <1..566
                     /locus_tag="AC16CY_09660"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3354096.1"
                     /translation="MSVAVSRRRFTADDYQRMGQAGILSDDDRVELIDGEVVAMTPLG
                     PRHGASIDRATRALVNAAGDRAIVRVQGAVRLDLFHEPEPDIVLLRPRTDYYATRQPG
                     PADILLIIEVAESSIDYDRDVKSRIYARSGIPEYWLVDLNDDVLVCHDEPKDGAYQRI
                     RQYRRDQAIAPELLPECMISADDLLGI"
     gene            572..1405
                     /gene="ttcA"
                     /locus_tag="AC16CY_09665"
     CDS             572..1405
                     /gene="ttcA"
                     /locus_tag="AC16CY_09665"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIR3354097.1"
                     /translation="MSYRSPLESRIAKKTTKAITDFNLIEDGDRIMVGLSGGKDSWAL
                     MQTLDNLRQRAPIRFSLVAVNVDSGYKDFKHGAIASACEERGWEYRIEHTSIGEVMDD
                     LLEANATPCSLCARLRRGVLYRIATEVGATKIALGHHLDDFVETLLLNLFFAGALKAM
                     PARLVSDNGEHVVIRPLVYVGEDEARLYCKEVGLPIIGCCCPACGDLSLQRQRVKRLI
                     LDLEREHSGVKQSMVKALGNVMPRHLLDTRLNPSGELRDSAALRLEDEARSPSVIQIQ
                     H"
     gene            1444..1896
                     /gene="dtd"
                     /locus_tag="AC16CY_09670"
     CDS             1444..1896
                     /gene="dtd"
                     /locus_tag="AC16CY_09670"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR3354098.1"
                     /translation="MRAVIQRVTSARVCVGDRVVGEIGRGLLVLVGVGRDDTGEDVTY
                     LAGKIRDARIFESEDGKPMDRSVADIGGSVLVVSQFTLYGDMRKGRRPSFDAAGSPEL
                     ARAHYEALVRELRAAGLTVSTGEFRAMMQVELVNDGPVTILLDSRRQS"
     gene            1896..2738
                     /locus_tag="AC16CY_09675"
     CDS             1896..2738
                     /locus_tag="AC16CY_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354099.1"
                     /translation="MKRACIFAAALLLGQAPAFGQQRPLLTEDPEPIGAGRILVEGGL
                     DYGRDVNYPVSGLDGHLFRGPLIGVSVGLSSIAELQIDGGFFNHLSITTRNPAPLSDM
                     VTATGDGTSSVEDFVIATKIRIAQETEGRPAFGLRFATKLPNSSNESGLGLDTTDFYA
                     SVIGAKTVRSVRFVGNIGLGILGDPTRGDRQNDVVTYGASFARALTDAAEIVGELNGR
                     ISTQGASTPPGTESRGTARIGARYTISSWRADAAVFFGLTSTDPSVGFAGGFTYVFNA
                     FQIP"
     gene            2738..3535
                     /gene="dapF"
                     /locus_tag="AC16CY_09680"
     CDS             2738..3535
                     /gene="dapF"
                     /locus_tag="AC16CY_09680"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR3354100.1"
                     /translation="MPQARVTKAHAYGNDFLCVPAGDAGSDRPALARAMCHRHHGIGA
                     DGVLIYTLRDGGAGMTLLNADGSPSELSGNGLRCLAALVAHVQAVPPGGIVTIDTDAG
                     LKMLKLLARDGERYTFRAALGHPTGPRRATIEVSGEQITASVLEVGNPQCVVLGPLPD
                     QARFNRLGPALSTHPMFAAGTNVEFAEIEARDRIRILIWERGVGPTTSSGTGSAAAAV
                     AAAAHGGAARSLDVVAPGGTQRVEWLDDGIYLTGWAEILFEGIWTGS"
     gene            3536..5053
                     /locus_tag="AC16CY_09685"
     CDS             3536..5053
                     /locus_tag="AC16CY_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3354101.1"
                     /translation="MRTFHGAVAAVETYSPAEERFNARRRAVGLWLGPLVLLAMLAAP
                     LPVPLPAHRLAAILAMMVVLWVTEALPLAVTALLGPVLAVLFGVAPVRTAFAAFADPV
                     IFLFIGGFMLAEAMFVHGVDRRIAYTALSWKWIGTSAPRVLLVYGAVAMLISMWMSNT
                     ATTAMMFPIGLSIVAHLARAGASGARQFGLVVMLITSFAASTGGLGTPVGTPPNLIGI
                     GMLEREANVHITFFQWMAIGVPAAVLMFAFVAAQFYFAGARNVRLGEESARLLRDELD
                     KLGRLSRGQINVLVAFGITVVLWVLPGLLALARLDETPFARAFADAVPESIAALVGAL
                     LLFVIPIDWRGKRFTLTWDEALRIDWGIVLLYGGGLAMGGLAFSTGLAEAMGRGITAW
                     LPSHGTVALTILFTATAIVLSEATSNTAAANMIVPVAIAVSQVAGVRPIEPVLGATLG
                     ASMGFMMPVSTPPNAIVYSSGFVPITAMMRYGIMLDLVGFVVIISLVLLLGPVIF"
     gene            complement(5122..5709)
                     /locus_tag="AC16CY_09690"
     CDS             complement(5122..5709)
                     /locus_tag="AC16CY_09690"
                     /inference="COORDINATES: protein motif:HMM:NF025704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HIR3354102.1"
                     /translation="MSLSTWRAGGAGLVVALLAVSCAAPPIKELDQARNAITTARDAG
                     AERFAPDEIAAADRELKLASQAVDARDYRLALNHALESREQAEAAAKSSTDTRTRLRQ
                     DGERSIEESRALIASTKARLDGPKAAQIPRRTRQAARQTLTDLENALQEADAALRKED
                     FKAAERTLASVKQRLARVVASLPKEPTAQSSRRSG"
     gene            5726..6400
                     /gene="radC"
                     /locus_tag="AC16CY_09695"
     CDS             5726..6400
                     /gene="radC"
                     /locus_tag="AC16CY_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR3354103.1"
                     /translation="MKGVAPHDRPREKLERLGLAALGDNELVALVLGSGSRGRNALQV
                     ANRLLEHVGGLHGLTRVTVDELRRVTGVGRVRAAQVLAAVEMGRRTLVRGDVDRPDLG
                     SPDRLAAYLLPLYGSGAIEHFGLVMLDSKSRLIRVKLVAGGSIDVTVVHPREVFREAA
                     AAAAAAIVLFHNHPSGDPTPSRDDFVLTARMVGAGKVMGIDVVDHLILADRRYYSLVE
                     AGQMPA"
     gene            6442..>6678
                     /gene="larC"
                     /locus_tag="AC16CY_09700"
     CDS             6442..>6678
                     /gene="larC"
                     /locus_tag="AC16CY_09700"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HIR3354104.1"
                     /translation="MKTLYFDCFSGASGDMVLGALIDAGVTLEDLRRALGDLAIAPDA
                     VWTERVTRAGISATKFNVRGEALPLDHADARHLGH"
BASE COUNT         1009 a         2217 c         2284 g         1168 t
ORIGIN      
        1 ccatgtcagt cgcagtcagc agacggcgtt tcaccgcgga cgactaccag cgaatggggc
       61 aggccgggat tctgtcggac gacgaccggg tcgagctcat cgacggcgag gtcgtggcga
      121 tgacgcctct cgggccgcgc cacggcgcga gtatcgaccg cgcaactcgc gccctcgtga
      181 acgcggccgg ggaccgagcg atcgtccggg tgcaaggggc ggttcgtctg gacctcttcc
      241 acgagcctga accggacatc gtgctcctgc ggccgcgaac cgattattac gccacgcgac
      301 agccggggcc cgctgacatt ctgctcatta tcgaggtggc cgagtcgtcg atcgactacg
      361 accgcgacgt caaatctcgt atctacgcgc gatccggtat tccggagtac tggctcgtcg
      421 acttgaacga cgacgtgctc gtctgccacg acgagccgaa ggacggcgcg tatcagcgca
      481 tcaggcagta tcggcgagat caggccattg cacccgaatt gctcccggag tgcatgattt
      541 cagcagacga cctgctcggc atctgaaccc catgtcctac cgcagccccc tcgagtctcg
      601 catcgccaag aagacgacca aggccatcac cgacttcaac ctgatcgaag acggggaccg
      661 gatcatggtc ggtctctcgg gtggcaagga cagctgggcg ctgatgcaga cgctcgacaa
      721 cctccgccag cgcgcgccca tccggttctc gctcgtcgcg gtcaacgtcg attcggggta
      781 caaggacttc aagcacggcg cgattgcgag cgcgtgcgag gagcgcggct gggaataccg
      841 gatcgagcac acctctattg gcgaggtgat ggacgacctt ctcgaggcca atgcgacacc
      901 ctgctcgctg tgcgccaggc tccggcgcgg cgtgctgtac cggatcgcca ccgaggtcgg
      961 cgccaccaag atcgctctcg gccatcacct cgacgacttc gtcgagacgc tgctgctgaa
     1021 tctcttcttc gccggcgcgc tcaaggcgat gcccgcccgg ctcgtctccg acaacggcga
     1081 gcacgtcgtc atccggccgc tcgtgtacgt cggcgaagac gaggcgcgtc tctactgcaa
     1141 ggaagtgggg ctcccgatca tcggctgttg ttgtccggcc tgcggcgacc tcagcctgca
     1201 gcgtcagcgc gtgaagcgcc tgatcctcga cctcgagcgc gagcattccg gcgtgaaaca
     1261 gtcgatggtg aaggcgctcg gcaacgtgat gccccggcat ctgctcgaca ctcgcctcaa
     1321 ccccagcggc gaactgcggg acagcgccgc gctgcgactc gaggacgagg ctcgatcccc
     1381 cagcgtgatt caaatacagc actgaacggc agtcctcggt ccacggggct gcaggctacc
     1441 ggcatgagag cagtcattca gcgcgtcacc tcggcgcggg tctgcgtcgg cgaccgggtc
     1501 gtcggcgaga tcgggcgcgg gctgctggtg ctcgtcggcg tcggccggga cgatacgggc
     1561 gaggacgtga cgtatctggc cggcaagatc cgcgatgccc gcatcttcga gagtgaagac
     1621 ggaaagccga tggatcgatc cgtcgccgat atcggcgggt cggtgctggt cgtctcgcag
     1681 ttcacgctct acggcgacat gcgcaaggga cgacggccgt cgttcgatgc cgcgggatcg
     1741 cctgagctcg cacgcgcgca ctacgaggca ctcgtgagag aattgcgcgc agccggtctg
     1801 acggtctcga cgggcgaatt ccgcgcgatg atgcaggtgg agctcgtcaa cgacggcccg
     1861 gtcaccatcc tcctcgacag caggcggcag tcctgatgaa acgtgcgtgc atcttcgcgg
     1921 cagccctgct gctcggtcag gcgccggcgt tcggccagca gcggccgctc ctcaccgagg
     1981 atcccgaacc gattggcgcg ggccgcatcc tcgtcgaggg cgggctcgac tacggccgcg
     2041 acgtgaacta tcccgtgtcc gggctcgacg gccatctgtt tcgtggcccg ctgatcggcg
     2101 tgagcgtggg cctgagctcg atcgccgagc tccagatcga cggaggcttc ttcaaccacc
     2161 tcagtatcac gacgcgcaac ccggcaccgt tgtccgacat ggtgaccgcg accggtgacg
     2221 gaacatcgag cgtcgaggac ttcgtgattg ccaccaagat ccggattgcg caggaaacag
     2281 agggccgccc ggcgttcggc ttgcgctttg ccacaaaact gccgaactcg tcgaacgaga
     2341 gcggcctcgg cctggatacg acggacttct acgccagcgt gatcggcgcc aagaccgtgc
     2401 ggtcggtcag gttcgtcggc aacatcggcc tcgggatcct gggagacccg acgcgtgggg
     2461 accggcagaa cgatgtcgtg acctacggcg cgtcgttcgc gcgcgccctg accgacgcgg
     2521 cggagatcgt cggagagctc aacggccgaa tcagcaccca gggcgcctcg acaccgcccg
     2581 gcacggagtc gaggggaacc gcgcgcatcg gcgcccgcta cacgatcagc agctggcggg
     2641 cggacgccgc ggtgttcttc ggcctcacct ccacggatcc gagcgtcggg ttcgctggcg
     2701 ggttcaccta cgtcttcaac gcgttccaga tcccctgatg ccgcaggctc gcgtcacgaa
     2761 ggctcacgcc tacggcaatg acttcctgtg cgtgcccgcc ggcgatgccg ggagcgatcg
     2821 tccggcgctg gcgcgcgcga tgtgccaccg gcaccacggc atcggcgccg acggcgtcct
     2881 gatctacacg cttcgcgacg gcggcgcggg catgacgctc ctcaatgccg atgggagccc
     2941 gtccgagctc tcggggaacg ggttgcggtg cctggccgcg ctcgtcgcgc acgtgcaggc
     3001 tgttcccccc ggcggcatcg tcacgatcga cacggacgca ggcctcaaga tgctgaagct
     3061 gctggctcgc gacggagagc ggtacacgtt ccgcgcggcc ctgggtcatc cgacgggccc
     3121 gcgccgcgcg accatcgagg tctccggcga acagatcacg gcgtcggtcc tcgaagtggg
     3181 caacccgcag tgcgtcgtgc tcggaccgct gcccgatcag gcccggttca accggcttgg
     3241 accggcgctc tccacgcatc cgatgtttgc cgctggaacc aacgtcgagt tcgccgagat
     3301 cgaggcgcgc gatcgcatcc ggatcctgat ttgggaacgg ggcgtgggtc cgacgacgtc
     3361 ctccggtacc gggtcggctg ccgcggctgt tgcggccgcg gcgcacggcg gcgcggcacg
     3421 gtctctcgac gtcgtcgcgc ctggagggac ccagcgcgtc gagtggctcg acgacgggat
     3481 ctacctgacc ggatgggccg agattctttt cgaagggatc tggacgggct cgtagatgcg
     3541 gacattccac ggcgcggtag ccgctgtcga gacctactcg cccgccgaag agcgcttcaa
     3601 cgcgcggcgg cgcgccgtcg gactctggct cggccccctg gtgctcctgg cgatgctcgc
     3661 ggctccgctg ccggtgccgc tgccggccca tcggctcgca gcgattctcg cgatgatggt
     3721 cgtgctctgg gtcacggagg cgctgccgct ggccgtcacc gcgctgctcg gcccggtgct
     3781 ggctgttctg ttcggtgtgg cgccggtccg caccgccttt gccgcctttg ccgatccggt
     3841 catcttcctg ttcatcggcg gcttcatgct tgccgaggca atgttcgtgc acggcgtcga
     3901 ccgccggatc gcctacacgg cgctttcgtg gaagtggatt ggcacgagcg caccacgggt
     3961 gctgctggtc tacggcgccg tggccatgct gatctccatg tggatgagca acacggcgac
     4021 aacggccatg atgttcccga tcggtctgtc gatcgtcgcg cacctggccc gtgccggggc
     4081 gagcggcgcc aggcagttcg gactcgtcgt catgctcatc acgtcgtttg cggcctcgac
     4141 cgggggactc ggcaccccgg tcggcacccc gcccaacctg attggcattg gaatgctgga
     4201 gcgtgaggcc aacgtgcaca tcacgttctt tcaatggatg gcgatcggcg tgccggcggc
     4261 cgtcctgatg ttcgcgttcg tggcggcgca gttctatttc gcaggagcca ggaacgtgcg
     4321 cctgggcgag gagagcgccc ggctcctgcg cgacgagctg gacaagctgg gccggctgtc
     4381 gaggggacag atcaacgttc tggtggcctt cggcatcacc gtcgtcctgt gggtgctgcc
     4441 ggggctgctg gcgctcgccc gtctcgacga aacgccgttc gcgcgcgcct tcgccgacgc
     4501 ggttcccgag agcatcgcgg cgctcgtcgg tgcgctgctc ctgtttgtca tcccgatcga
     4561 ctggcggggc aagcgattca cgttgacctg ggatgaagca ctccggatcg actggggcat
     4621 cgtgcttctg tacggcggcg ggctcgcgat gggcgggctc gcgttttcaa ccggcctggc
     4681 cgaggcgatg ggccgcggca tcaccgcctg gctgccgtcg cacggcacgg tggcgctgac
     4741 gattctcttc acggcgacgg cgatcgtgct gtcggaggcg acctcgaaca cggcagccgc
     4801 aaacatgatc gtgccggtgg ccatcgccgt ctcgcaggtg gcgggcgtcc gccccatcga
     4861 gcccgtgctc ggtgccaccc tgggcgcgag catggggttc atgatgcccg tttccactcc
     4921 gccgaacgcg atcgtttaca gctccgggtt cgtgccgatc acggccatga tgcgctacgg
     4981 catcatgctc gacctggtcg gcttcgtcgt gatcatcagt ctggtactcc tgctcgggcc
     5041 ggtgatcttc taggcgcgcc ttcagatcga tgtagcggac caggagccgg ctgcgggcgt
     5101 agcagttttt ccgtacccag gctatccact gcgtcgcgaa gattgcgcag tcggctcctt
     5161 cggaagcgag gcgacgaccc gcgccagacg ctgtttcacc gaggccaacg tgcgttccgc
     5221 ggccttgaag tcctctttcc gcagggctgc gtccgcttct tgcaatgcgt tttcgaggtc
     5281 ggtcaacgtc tgccgtgcgg cctggcgggt gcggcggggg atctgcgcgg ccttgggccc
     5341 gtcgaggcgc gctttggtgc tggcgatcaa ggccctcgac tcttcgatcg accgttcgcc
     5401 atcctgacgg agccgggttc gagtgtcggt ggacgacttg gcggcggcct cggcctgttc
     5461 gcggctttcg agagcatggt tgagagccag gcggtagtca cgcgcgtcaa ccgcctggct
     5521 cgccagcttg agctcgcggt cggccgcggc gatctcgtca ggggcgaagc gttcggcgcc
     5581 cgcgtcccgg gccgtagtga tggcgttccg ggcctgatcg agctccttga tcgggggcgc
     5641 ggcgcacgaa accgcaagca gcgcgacgac gagtccggcg ccgccggctc gccacgtcga
     5701 gagagacatg aaggattgag tatagatgaa gggtgtcgca cctcacgacc gtccgcgcga
     5761 gaagctggaa cgactcggac tcgcggcgct cggtgacaac gagctggtcg cgctcgttct
     5821 cggcagcggc tcgcgcgggc gaaatgcgct gcaggtcgcc aatcgcctcc tcgagcacgt
     5881 gggcggcttg cacggactga cgcgcgttac cgtggacgag ctgcgccgcg tcaccggcgt
     5941 cggcagggtg cgggcggcgc aggtgctcgc cgcggtcgag atgggccgcc ggacgctggt
     6001 gcgcggcgac gtcgatcggc ccgatctcgg atcgccggac cggctggcgg cgtatctgct
     6061 tcccctgtac ggttcgggcg cgatcgagca tttcgggctc gtgatgctcg acagcaagag
     6121 ccggctgatc cgggtgaagc tggtcgcggg cggatcgatc gatgtcacgg tcgtgcaccc
     6181 gcgcgaagtg ttcagggagg ccgccgcggc ggcggccgcg gcgatcgtct tgttccacaa
     6241 ccacccgtcc ggcgacccca cgccgagccg cgacgatttc gtgctgacgg ccaggatggt
     6301 tggcgccggc aaggtgatgg ggatcgacgt cgtcgatcac ctgatcctgg ccgaccgccg
     6361 gtactactcg ctggtcgagg ctggccagat gccggcctga gcgcgatcgc ccgtcgtacg
     6421 actgctataa gattggaagt cttgaagaca ctctacttcg actgcttctc tggcgcgtcc
     6481 ggcgacatgg ttctgggtgc gctcatcgac gccggcgtca cactcgaaga cctgcgccgc
     6541 gcgcttggcg acctggcgat cgctcccgat gcggtctgga ctgagcgcgt gacacgcgcc
     6601 ggcatcagcg cgacgaagtt caacgttcgc ggcgaggcgc tcccgctgga ccatgccgac
     6661 gcccgtcacc tggggcac
//
LOCUS       DBNGSH010000426         2422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_548481, whole genome shotgun sequence.
ACCESSION   DBNGSH010000426 DBNGSH010000000
VERSION     DBNGSH010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_548481"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC16CY_09705"
     CDS             complement(<1..190)
                     /locus_tag="AC16CY_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354105.1"
                     /translation="MTVLAGAGLAAQAPPILPSTTLVQAGRASLYPTVSLQDVGTDSN
                     VYLDSVAPKEDFTYTLTPR"
     gene            complement(386..1072)
                     /locus_tag="AC16CY_09710"
     CDS             complement(386..1072)
                     /locus_tag="AC16CY_09710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3354106.1"
                     /translation="MALFDREETDLGSDVASRALSVTPVENDFVEGVYEKLAKVYDLT
                     FGPTLHPGRLVALETMGLRPGDRILEVGVGTGINAPLYPRNCHVTGVDFSASMLERAR
                     ERVVRKGLRNVQLFEMDAASLAFPDNSFDVVYAPYVISVVSDPVQVVREMRRVCRPGG
                     KFIVLNHFRSPNRLLARVERAISPFTVHIGFKSDLDLPGFLAQAQLRPVSVKKVSFPR
                     IWSLVTCIKE"
     gene            complement(1229..>2422)
                     /locus_tag="AC16CY_09715"
     CDS             complement(1229..>2422)
                     /locus_tag="AC16CY_09715"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR3354107.1"
                     /translation="MSAVTGSHLSAQPNAGQPRELDGRTIYLTSPEYMASYARRFVER
                     RVRLVGGCCGTTPAHVEQIRSAMSRVSPRVFTGSAGPPTDRRAAPAGESTPRETVQPR
                     AEKSDLSRALADRRWVTLVEVIPPKGHDADAAIEQARVLRERGVNAVLVADGPSGPRL
                     SGLSLAALIARHARIDVVLQYSSRDKHLLGMQSELLGAYAMGLRNVVVVTGDPQPVGD
                     YADATAVIDVDSVGLVNAVSRLNRGLDVGGQAIGRPTAFFLGVAVNPGADDLDGELRR
                     FDHKVEAGAEYVICRPVFDVKTFERAHRRLEASGLPIVLSLRPFASVLEAEYLANEVP
                     GVRVPQDVIERMRRASGEDAAAAEGVAIAGEVGRALKSLVGGVSISSPSVRFDLGLAL
                     LQRLH"
BASE COUNT          441 a          789 c          825 g          367 t
ORIGIN      
        1 ggcgcggcgt gagcgtatag gtgaagtcct ctttcggagc gacgctgtcc aggtacacgt
       61 tggaatcggt gccgacgtcc tgcagcgaaa ccgtcgggta cagcgacgcg cgcccggctt
      121 gcaccagcgt ggtcgagggg aggatcggcg gggcctgtgc ggctaggcct gcgcccgcca
      181 gcaccgtcac gacggcggtg acgagggtgc gagggtgcga gggtgcgagg gtgcgagggt
      241 ggtaagtcac tgatcggatg aagcttcgcc ccctggctca cacacggagg cctgaaaggg
      301 cgcgagttgc tcagctgatg tatcgacccc gagggacggg agccttaggc gaaaacggcg
      361 gtgacgggcc gtgaaaccgg cgaagtcact ccttgatgca ggtcacgagc gaccagatgc
      421 gcggaaagct cactttcttc acggatacgg gacgcaattg agcctgggcc aggaagcctg
      481 gcaggtcgag atccgactta aacccgatgt ggaccgtaaa tggcgagatg gcccgttcca
      541 cgcgagccaa tagacgattt gggctacgga agtgattaag gacgatgaac tttccaccgg
      601 gccggcagac gcgccgcatt tcgcgcacga cctggaccgg gtcggacacc acgctgatga
      661 cgtacggggc gtacacgacg tcgaaggagt tgtcgggaaa cgcgaggctg gcggcatcca
      721 tctcgaacaa ctggacgttt cgaaggccct tgcgcaccac gcgctcgcgg gctctttcga
      781 gcatcgacgc cgagaaatcc actcccgtga cgtggcaatt ccggggatag agaggcgcat
      841 tgatgcccgt gccgacgccg acctcgagga tccgatcgcc gggcctcagc cccatggttt
      901 cgagggcgac gaggcgcccg gggtgcagcg tcggaccgaa cgtcaggtcg tagaccttgg
      961 ccagcttctc gtagacgccc tcgacgaaat cgttctcgac gggcgtcacc gacagcgcgc
     1021 gtgacgctac gtcggaccct aaatccgtct cttcgcgatc gaacagcgcc attcgggact
     1081 cctcacggcc agtcgtgacg tctcggggcc gtcggcacta tacagggccg gggaggcccc
     1141 gacaagcgat gtttacaagg ggcaagggtg cgagggtacg aaggtgcgag ggtgcgaggg
     1201 cacggggtgc ggcagcgtgg gtacccaatc agtgcagtct ctgcagtagg gccagcccga
     1261 gatcgaagcg aacagaaggc gatgagatgc tcacgccgcc gacgaggctc ttgagcgcgc
     1321 gaccgacttc gccggcaatc gccactcctt cggccgccgc ggcgtcctcg ccggaagcac
     1381 ggcgcattcg ctcgatcaca tcctgcggga cgcgcacgcc aggcacctcg ttggccaggt
     1441 attcggcttc gagaacgctc gcaaacggcc ggaggctcaa tacgatcggc agcccggacg
     1501 cctcgagccg ccgatgcgct cgctcgaacg tcttcacgtc gaacacgggc ctgcagatga
     1561 cgtactcggc gcccgcttcg accttgtggt cgaagcggcg gagctcgccg tccagatcgt
     1621 cggcgccagg gttcacggcg acgccaagga agaacgccgt cggacgtccg atggcctgcc
     1681 ctccgacgtc caggcctcta ttcaggcgcg acacggcgtt gaccagacca accgagtcca
     1741 cgtcgatgac cgccgtcgcg tcggcatagt cgccgaccgg ctgcgggtcg cccgtgacga
     1801 cgaccacatt gcgcagtccc atcgcgtacg cgccgagcag ctccgattgc atgcccagaa
     1861 gatgcttgtc gcgcgacgag tactgcagca cgacgtcgat gcgcgcgtgc cgcgcgatca
     1921 acgcggcgag cgacagcccg ctcaggcgcg gtccgctcgg accatccgcg acgaggaccg
     1981 cgttcacacc ccgctcccgc agtacgcgag cctgctcgat cgcggcgtcg gcgtcgtgtc
     2041 ccttcggagg gatcacctcg acgagcgtga cccagcgccg gtcggcaagt gcacggctga
     2101 gatccgactt ctccgcgcgc ggctggaccg tctcacgcgg ggtggactcc cctgccggcg
     2161 ccgcgcgacg atccgtcgga gggccagccg atccggtgaa cacgcgcggc gacacccgag
     2221 acatggcgga tcggatctgc tcgacgtgcg cgggcgtcgt gccgcagcag cctccaacca
     2281 gccgcacacg cctctcgacg aagcggcggg cgtacgacgc catgtattcc ggcgaggtca
     2341 ggtagatcgt ccgtccatcg agctcgcgcg gctgaccggc attcggctgc gccgacaggt
     2401 gcgacccggt cacggctgac at
//
LOCUS       DBNGSH010000427         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2755977, whole genome shotgun sequence.
ACCESSION   DBNGSH010000427 DBNGSH010000000
VERSION     DBNGSH010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2755977"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC16CY_09720"
     CDS             <1..342
                     /locus_tag="AC16CY_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354108.1"
                     /translation="GQVGLPTSGSALRVTWLATQAGTALTGPATLVKPAVNVPATGTL
                     SGTLNGSQLALAYLAPGGSVPGFPACEVSGTGTATFQGDTITGTLALNFTACAGSGLE
                     PTGSDLLALTK"
     gene            complement(374..1615)
                     /gene="ispG"
                     /locus_tag="AC16CY_09725"
                     /gene_synonym="gcpE"
     CDS             complement(374..1615)
                     /gene="ispG"
                     /locus_tag="AC16CY_09725"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3354109.1"
                     /translation="MISEVPTARLHRTVGVRVGRVQVGGGAPVAVQSMTMTDTADAAA
                     TARQCIELAEAGSELVRVTVNLPEAAAAVPEIKRRMLDAGCTAPLIGDFHYNGHLLLT
                     RFPDCAAALDKYRINPGNVGTGRRRDEQFATICSVAIDHGKPVRIGVNGGSLNQELVV
                     SKMQENTSRALGRTSEEILNECMVMSAIESTALALDTGLRKDQIIISCKVSRPRDLLA
                     IYRELARQTDQPLHLGLTEAGMGTKGLVWSASAIGILLNEGIGDTIRVSLTPRPGGDR
                     REEVYAACELLQALGLRSFAPSVTACPGCGRTTSSTFQELAERTQDYIRAQMPEWKKK
                     YEGVETLTLAVMGCVVNGPGESKAANIGISLPGTGESPNCPVFIDGEHYTTLRGTYDE
                     LAEGFRALVDNYVATRYAARN"
BASE COUNT          320 a          663 c          651 g          358 t
ORIGIN      
        1 gggcaggtcg gcctgccgac gtcgggatcg gcgctccggg tgacgtggtt ggcgacgcag
       61 gcgggaacgg ccctcaccgg tccagcaacg ctcgtcaagc ccgcggtgaa cgtgccggcg
      121 actggtacac tgagcggaac attgaacggc agccagctcg cgctggccta ccttgcgccg
      181 ggcggcagcg tgcccggatt ccccgcctgc gaagtctccg gcacgggaac cgctaccttt
      241 caaggcgaca ccatcacggg gacgctcgcg ctgaacttca cggcgtgcgc cggctccggc
      301 ctcgagccga ccggcagcga tttgctcgcg ttgacgaagt agcggggtca gaccccttca
      361 cgggtctgcg cgactaattc ctggcggcgt agcgcgtcgc gacgtagttg tccacgaggg
      421 cgcgaaagcc ttcggccagc tcgtcgtagg tgccgcgcag cgtcgtgtag tgctcgccgt
      481 cgatgaacac gggacagttc ggagactcgc ccgtgccagg caggctgatg ccgatgttgg
      541 ccgccttgga ttcgcccggc ccgttgacga cgcagcccat gacggcgagg gtgagcgtct
      601 cgacgccctc gtatttcttt ttccactccg gcatctgcgc gcggatgtag tcctgcgtcc
      661 gttcggccag ctcctggaac gtggagctcg tcgtgcggcc gcagccaggg cacgcggtca
      721 cgctcggggc gaacgagcga aggccgagcg cctgcagcag ctcgcacgcc gcgtacactt
      781 cttcacgccg gtcgccgccg ggccgcgggg tcaacgacac gcggatcgtg tctccgatcc
      841 cttcgttgag caggatgcca atcgccgacg ccgaccagac gagccctttc gttcccatgc
      901 ccgcctcggt gaggcccagg tgcagcggct ggtccgtctg ccgcgcgagc tcgcggtaga
      961 tcgccagcag atcgcgcggg cgcgacacct tgcacgagat gatgatctgg tctttgcgaa
     1021 gccccgtgtc gagcgcgagg gccgtcgatt cgatcgcgga catcaccatg cactcgttga
     1081 ggatttcctc ggacgtcctc ccgagtgccc gcgacgtgtt ctcctgcatc ttggagacca
     1141 ccagctcctg gttgagcgag ccgccgttga cgccgatgcg aacgggcttg ccatggtcga
     1201 tcgcgaccga gcagatcgtc gcaaactgct cgtcccgccg ccggcccgtg ccgacgttgc
     1261 cggggttgat gcggtacttg tccagcgcag cggcgcaatc aggaaagcgc gtcagcagga
     1321 ggtggccgtt gtagtggaaa tcgccgatga gaggtgccgt gcagcccgcg tccagcatgc
     1381 gccgcttgat ctcgggaacg gcggcagccg cttccggaag gttgaccgtg acgcggacca
     1441 gttcggagcc tgcctccgcg agctcgatgc actgacgcgc cgtcgccgcg gcgtccgccg
     1501 tatcggtcat cgtcatcgac tggacagcca ccggcgcgcc gccgccgacc tggacccggc
     1561 caacgcgcac accaacggtc ctgtgcagcc ttgcagtagg cacctcagag atcatagccg
     1621 ttcacgcttc tcgcttctca cttctcggtt cgtgttcagt ttcaggttcg tgttcaatgt
     1681 tcggggttct gggttcggcg ttccttccgg gctcttcgca gggccgggcg cgccgaaccc
     1741 gaacctgaac ctcaaagctg aacacgaacc ccgaaattga agacgaaccg agaagcgaga
     1801 agtgagaagt gtgaactgag ggatcttcag gcttctttcc acccgtcctc gaggagaatc
     1861 cctcggattt gatccgctcg cgccatcgca agatcgagcg tgtcgtagtt ctccgcccgg
     1921 tccatcaccc cgtcgaagaa ccacgtcacg gtggtgtgcc tgtcgccagg gaagacggtc
     1981 gcatgagcgg tg
//
LOCUS       DBNGSH010000428        35952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_208926, whole genome shotgun sequence.
ACCESSION   DBNGSH010000428 DBNGSH010000000
VERSION     DBNGSH010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 35952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 35952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35952
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_208926"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..8836)
                     /locus_tag="AC16CY_09730"
     CDS             complement(<1..8836)
                     /locus_tag="AC16CY_09730"
                     /EC_number="1.14.-.-"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0019184 - nonribosomal peptide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0044550 - secondary metabolite biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HIR3354110.1"
                     /translation="MTRPSKSPATRGRVADIYPLTPLQRGLLFHTLYSPESGVYVERL
                     GYTIEGKLDAAAFQRAWQLVVDRHAVLRTAFALEGEKGPLQLVRSRVDLPWINEDWRD
                     VPAAEHQSRLADFVADERQRPLRLDRAPLMRMALIRLSEDAYRFFWTFHHVLLDGWSV
                     AVVLHEVQVFYDALRRGVEPQVPPPRPFRDFVAWLMARNPTAAETFWRERLKGFEAPT
                     PLGVNRRRATVGTREAFGSERVQISAARTAALQAFARRHNLTLNTVVQGAWAILLSRY
                     GGTDDVVYGTTSSGRPADLSGSESMVGLFITMLPVRARVLADVPVLSLLTSIQRQQVE
                     QREYEYASLVDIQRWSGVPSATPLFESAFVFENYPVRPFSDGNAGGLAVREDVAESRT
                     NYPLIVVVGPGERLSLRIDYDGSRFDAAAVGRMLAHLEMLLDGLLSDANPRIDDIPML
                     TTAERQQLLVDWNDTQVSLTGPRCVHQMFEEQAAKTPDSVALVFEDRSVTYSVLDRWA
                     NRLAVRLAELGVKPNALVGLFMERGPEMLAAILGVLKAGGAYVPLPRDYPKQRLQYML
                     EDCGAELVLTQEHLREEIAAGSATVECVDHGGRDDADYTVNRRTDVTPRHLAYVMYTS
                     GSTGRPKGTMIEHASVANYLDWCTRTYPLAEGSGAPVQSSLGFDATITSLISPLIAGR
                     RVVLLPEAREVEALGEALASGSDFGLVKLTPAHLKMLAATIPPEQAAGGTRAFVIGGE
                     ALTATDIDFWRTHAPRTRLINEYGPTETVVGCCVYEVSEDTPTSGAVAIGRPIINTQL
                     YVLDRRMQPTPVGIAGELYIGGAGLGRGYLKRPELTAEKFVPHPFSELPGERLYRTGD
                     LSFHGSDGELEFLGRIDDQVKLRGFRIELGEIEEVLRQQPGIGDAVVVVYTAAPGDDR
                     LVAYVVAAEGAAAASGGALREALKDRLPDYMVPTTFVTLAALPLTPNGKVDRRALPAP
                     EPQAIESTYVAPRSPMEEILADVWASVLGVPQVGAHSDFFELGGHSLLATQVGARVRA
                     LFGVDLPLRTLFEQPTVAAFARVIEAAHQGEATPVAPPMARRDGEGDAPLSYAQERLW
                     FLDQLEPGGSAYVVPSALRLTGPLHLAAFAAAYREVIRRHEGLRAVVGRDGEGRPVQR
                     ITPVSRISIELADLSRLAPDARHETASRLATQAAQQPFDLATGPLVRAIVVRLSDADH
                     MVLFSAHHIVTDGWSAGLLVGEVGTLYEEFIDGRPSRLQEPTLHYADYAVWQRDWLAG
                     GAMDAQLAYWRTRLAGAPRVLELPTDRPRPAVQSFAGGSVPVEISTETAAALGALSRR
                     EGATLFMTLLAVFQTLLYRYAGQPDVLVGTPIAGRTRAETEGMIGLFVNTLVLRAELG
                     SDPSFRALLAQVRETALGAYAHQDVPFERLVDELKVERDLSRTPLFQVMFALQNVPAA
                     ALHIRDVSLAPVVVEQGTSKFDVALSLFESRKGIVGALEYNPDLFDAATIQQMAGHFQ
                     ALVAGVVANPDARVSALPLLNADERARLLVEWNDTATAPPSATIPEVFEAQVARTPDA
                     VAVVAETTTLSYRELNTQANRLARRLRDLGVGPGVRVAIWLERSADLVVAILGVLKAG
                     AAYVPVDPAYPTDRVRLMLEDSAAAAVVTGKAFASMVPEGTAVVHIGAVTSAGETESV
                     ENLSLAVGPTALAYVIYTSGSTGRPKGVCVPHGCVTNFLASMARTPGLAADDLLLAVT
                     THSFDISVLELLLPLTVGARVWVAAAATVSDGQQLEAAISRSGATLMQATPATWRLLF
                     EANWPGARGLTVLCGGEPLPAALADRLNACVGALWNMYGPTETTVWSTCGPVASSAPI
                     TVGRPIANTQVYVLDRQLEPAPVGVAGELYIGGAGVTAGYWQRPDLTAGPFVPDHLSG
                     AAGARLYRTGDLARWRPNGTLEHLGRADRQVKVRGHRIEPGEIEAVLERHVLVEACAV
                     EPREVSGDVQLVAYVVGDLATADSVVSLRDFLRASLPDYMVPARFVTLDALPLTPNGK
                     VDRRALPEPDQSRTELDEFEPPRTPTERALAALWSEVLGIETIGVRDDFFRLGGHSLL
                     ATRMVSRVRALFNVEYPLRAVFDEPTVAGQARYVERLLTDGDHQLEIPLSPVGRDVDL
                     PLSHAQERLWFLDRLDSGSAAYNIPSAVRLKGPLHLAALSHAVDEILRRHAILRARFA
                     TGKDGPRQDIGAATPGALAVVDLSALPDAERTTQALRLAADEANRSFDLTRGPLMRAT
                     VATLAESEHVLLLTIHHIVSDGWSMGIVIGELSRIYAAFARGETSPLADLNVQYVDVA
                     VWQRQWLDAGALDQQLGYWRNRLSGAPQSLELLTDRSRPPVQSFRGASRPVTLSKELS
                     EAVRALSATQGATPFMTLLAGFFSLLFRYTGQDDILVGTPIAGRSRTETENLVGLFVN
                     TLVMRADLAGDPSVREIVTQVRDTALDAYAHQHLPLEKLLDELDVPRDLSRTPLFQVM
                     FALQNAPAGGVEVAGLSMSPVEVEQKTAKFDLTLNLAESPGGFYGVLEYNTDLFDAST
                     IERMAGHFVAVLEGMVTDAGRRISEIPLLSADERARQLREWNDATVDYATDMCLHEWF
                     ERQVVRTPDRVAVILDGHQITYGALSTRANQLAHHLRSLGVGPEVLVGLYMERSIELV
                     VGILGILKAGGAYVPLDPVHPPERLAFMVADARIDVLVTEDRLSAGLPETKAHVLSLD
                     GAWEAISRERATVPAVSMSVDNVAYVIYTSGSTGTPKGTPVTHRNVARLFAATDDWFE
                     FGAQDVWSLFHSYAFDFSVWELWGALCYGGRLLVVPYGVSRAPDEFIDLLARERVTVL
                     NQTPSAFRQLVAAEGKRRLDGAMSALRYVVFGGEALDVRHLAQWMSRHGECPRLVNMY
                     GITETTVHVTYRVITQADLDTPHVDSPAGRPIPDLQVYVRDEHGELLPAGLPGEIYVA
                     G"
     gene            complement(8837..10603)
                     /locus_tag="AC16CY_09735"
     CDS             complement(8837..10603)
                     /locus_tag="AC16CY_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HIR3354111.1"
                     /translation="MRAPAILRAAVPCIALAAGCGRATGPDVPLSQVVASETSLKFTI
                     TLPDGAAREALQGRLLVLVSAAAAGEPRFQVNDGPQSAQVFGVDVESWMPGSRRTVDG
                     AAFGYPVSGLAGLPPGNYRVQALLNRYRTYTRADGHTVSLPPDMGEGQQWARKPGNLY
                     SLPIERRVDPAKDDVVELTLDQTIPPLDPPKDTPWVKHVRIKSTLLSKFWGTDVYLGA
                     HVLLPAGYAEHPEVRYPLAVFHGHFTRDFDGWRTDPPDEKAPCVYNARYGVDCYNRVR
                     EEAAYALYKDWISPGFPRMLVVQIQHANPYYDASYAVNSANLGPYGDAITHELIPYIE
                     KQFRGIGEGWARFLYGGSTGGWEAMAAQVFYPDQYNGAFVACPDPIDFRAYTVVNLYE
                     DANAYWIDGPFRRTPRPGRRNYLGHVSGTLEQLNHLELALASRGRSGGQWDIWQAVYS
                     PVGNDGYPRPIWDKRTGAIDRSVADYWKENYDLSWILRRDWSTLGPKLRGKLHIYVGD
                     MDNYYLNNAVYLTEQFLKSATPPYAGVVDYGDRAEHCWNGDHERGIAYSQLRYVQMYV
                     PRILERIKETAPPGADPSSWRY"
     gene            complement(10623..11927)
                     /locus_tag="AC16CY_09740"
     CDS             complement(10623..11927)
                     /locus_tag="AC16CY_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR3354112.1"
                     /translation="MLCLGGLSIATGVLFNKWVIEAAFVSDHHIANSTMLAGIAVAQI
                     VLVALGAYLVVRRHSIRLSVKVPGRGEAVLLLASIVTPLLVIEAVLRVFDYNPFGELA
                     NGRELILRPSSVKERIYEATPFAEGYAWGTHVRINSRGFRDREYELQKPAGTSRVVVV
                     GDSVAFGNSLGPNESFARQLDDMVRLKGRAVEVLNMALGGYDTLQEVATLKDIGIQYK
                     PDVVVVGYVVNDVEVASTNVEYILRARRYGSPIYRIRLFQFVRSRLDTAGLGRDNMRA
                     NLEANFAADNRDYIADTTGDAELGAIMSRLKTEMETNGSAASRFRVANYLSHARIGKL
                     RYALGELGRLQRQHGFRVLVVIVPYLVEEKRYESTYRLIYDIVEHESTRAGFEVIRLE
                     GSFEAAGYDQLVIAGWDRLHPNALGHTLIAKALYDQLYDHDR"
     gene            complement(11991..21104)
                     /locus_tag="AC16CY_09745"
     CDS             complement(11991..21104)
                     /locus_tag="AC16CY_09745"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIR3354113.1"
                     /translation="MRFAASFAQERLWFLDRLEPGSAAYNIPAAIRLSGPLRAEALNG
                     ALREVVRRHEALRTTFTESEGRAQQVIDAGEPCALCRVDVSELPETGREKEVRRLARQ
                     EAARPFDLSRGPLMRATLIRLADQEHALLLTMHHIVSDVWSIRVLLSELSSVCETFSR
                     GRPSAFPELPIQYADYAHWQRQWLQGDVLEAQLSYWRDRLRGAPRLLELPADRPRPAV
                     QSTAGATEPVWLPRALSEALVALSRREGATLFMTLLAAFQALLHRYADQEKVLVGTPI
                     AGRTRTETEGLIGLFVNTLVLRADVRHDMSVQELLAEVRVAALGAYANQDLPFEKLVE
                     ELEPIRDLGKMPLFQAMFIFQNTPQMAIDLAGLKASALDDTSAHVRSDLDLYAWESGD
                     GIRGTFVYRTDLFEAPTIARLCRRFITLLESIVRDPAAPIDALRLDPDLELASIPLSA
                     SHDSRGSLSYHQERMWFIDQFETGNVYESHPVYHNLPLVLHFQGGVDADLLQRAINLV
                     TGRHDVLRTRFVTRDGHAYQEVVPHTDLELNVRDVAGDPDRDASELVVQFALEASQIP
                     IALDRDRLIRATLFRLADATAVLVVTVHHSAADTWSLRVLAQEIAESYNAQTEKRPAR
                     FADLTVRYIDHANWQRTLPEDVVEQLLFYWKRQLAGPLQALTLPTRVGRPAVHTYSVG
                     RRTFAFSGRLSSQIDARGRAEHTGTFEVLLAAFNVLLHRYSGHEEIVVGTSVPCRRHA
                     AVRNAVGPFANLLVLRSNLGGRPTFGAFLKQIIRTVDDARRHEDMPFDWLVKELNPQV
                     DMSRTALFDVLFRFDDAPPAAITVGQTKAILVDTNLGHGKYDVNLSVQRTAEGFVGTW
                     VYNTDLFDEFMIEQLAGHFEALLECFASGSDVPVNDVDLLSQAEKRTQLVDWNRTAAE
                     YPREKTIHSIFEEQVARYPDRVAVVFQHTQLTYRQLDEESNRLANYLREQGVGRETLV
                     ALCLARSPDMIVAILGVLKAGGAYLPIDPDYPDERRSYIVNDSGVSHAISTSSSIGIL
                     ADSVSSLILLDRDREAIGSRSSQPPAAHAVPDNIAYCIYTSGSTGRPKGVLLEHRQVV
                     RLMVNDHMQFAFTEEDVWSVFHSYCFDFSVWEMYGALLYGGRLVIVSEGVNKDPQQFL
                     GLILSEEVTVLNQTPSAFQQLMHHALSQRRTSVALRYIIFGGEALAPRQLQPWNRAYP
                     EVKLVNMYGITETTVHVTFKGLEAEDLEKDGSNIGVPIPTLQTYLVDSGMRLLPVGVP
                     GELHVGGAGLARNYLNRPELTAERFIPHPFGGPAGARLYKSGDLGLYLPNGEMEYLGR
                     RDHQVKIRGFRIELGEIEGALSRHPALRAVAVVAREDTPGQVRLVAYTVAEAEPPGVT
                     ELRDFLKTSLPDYMVPAAFVALDALPLTSNGKVDRRALPAPETQALESAHVAPRSPVE
                     EILAGVWASVLGVPQVGVQSDFFELGGHSLLVTRVMSRVAQVFEVDLPLRTLFEHPTV
                     AGLAAQVEAAKQGGAVVRAPSIHRRVDRGSAPLSFAQERLWFLDQLEPSGSAYVVPSA
                     LRLTGTLHVASFAAAYREVVRRHESLRTVIVTTGDGRGEQRITDGESIALEFVDLSSL
                     APIAREEEMRRLALLMARRPFDMASGPLVRATVLRLEDETHVALFSAHHIVTDGWSMG
                     LIVRELTTLYPAFITGHPSPLAEPPIQYADYAVWQRNWLDAGAMDAQVSYWSDRLAGA
                     SGLLELPTDRPRPPLQGLAGASEPVRIAKDLSEALQVLSRREGVTPFMTLLAAFQTLL
                     YKYSGQKTVLVGTPIAGRTRAEAEGLIGLFVNTLVMRADFDGALTFRDVLAQVRETAL
                     GAYAHQDLPFEKLVDELEVERDLSRTPLFQVMFALQNTPTETLSLPGLSLLPVEIEQA
                     TSKFDMTLSLAESPSGIRGTLEYSSDLFDAATIRRMVGHFTQLLEAAVAEPTRPVATL
                     ELLSTAERTQLLETWNATEEMYRSDACIPDVFQAQVALTPDAVAVVFEGRQLTYRQLD
                     DRAARLASYLQSVGVGPEVRVGLCLDRSLDLVVGMLGILNAGGAYVALDPSYPEQRLR
                     VILEDADVALVLTQAHLAHRFGADVQVLCIDADWPANAPTADCSASHSLDPRNLAYVM
                     FTSGSTGRPKGVQIAHGSVVNFLETMRAVPGLDRDDTFVSVTTISFDISVLELLLPLT
                     TGARLVVAGPDDVRDGGQLAALVERHQATAMQGTPATWRLVLDNGWSGGASLKALCGG
                     EALSQDLASRLLSGARSVWNMYGPTETTVWSTLDHVEDRDAPTSIGHPIANTKVYVLD
                     RETRPLPIGVPGELHIGGAGVSRGYRKRPDETAARFVPNPFDVAGGSRLYRTGDRVRY
                     RPDGRIEFLGRIDHQVKIRGHRIELGEVETVLGQHQSIRDAVVVAREDAPGHTRLVAY
                     MLASGDQPLSVTELQRHIRASLPEYMIPAAFVTLDAWPFTPNGKIDRRALPAPEQSRP
                     ELETAFIAPRDPREKTLAAIWSRVLGVDQLGVHDSFFHVGGDSILAIQIVARANEAGL
                     RLTPRQIFEHQTIAELAAVAGVGEVAAEQGAVTGAVALTPIQRWFFESPPAEPHHFNQ
                     ALLLEARARLDPACLGQSVASLFLHHDALRHRFELRDGEWHQESMTPDGRSPFSTVDL
                     SSLAPAERSAEMTRQIEALHTSFDLGRGPLFHVRHFACGDEPDRVFLIAHHLVVDGVS
                     WRILLEDLLTAYDQAIRGVSATLPRKTTALQHWARKLYEYAQSGALNEEVAYWQQQVT
                     GEAPRLPVDRTDGPNTIASRAVVSATLTEEDSHALLTEVPEAYGTQINDVLLTALLQA
                     FAPWTGSRALVVDLEGHGRESPFDDLDVSRTVGWFTTIFPVRLELPAKEDPGSALTAI
                     KEQLRQIPQRGIGYGLLRYSEPARIAQLGDAPAEVIFNYLGRFDQTLAANTPFRFATG
                     SSGPAQSPRARPRHLMEVSGIIVDGRLQLSLQYSENVHARATMQGLVDGIADALRGII
                     AHCLSPDAGGASPSDFPLADLDQATLDRLLQGQEY"
     gene            complement(21101..22999)
                     /locus_tag="AC16CY_09750"
     CDS             complement(21101..22999)
                     /locus_tag="AC16CY_09750"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0019184 - nonribosomal peptide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0044550 - secondary metabolite biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HIR3354114.1"
                     /translation="MTREGIVDWFDRAAESFSETTAIECGERRLTYGELRRKADRLAR
                     LFLSAGATKGSVVAILVEDRVQVIVGMLASLKAGLAFAPMDSTMPPKRLEGIVSELAP
                     AWSLLACGTGAHVREALNDRHPGSRVVCLDEACERAHGVIHLEDESPGDARLPIEVAP
                     DDMCYVYFTSGSTGRPKGIAGRRKGLDHFIGWEIKTVGAGHGTRVSQLTTPSFDPFLR
                     DVFVPLCAGGTVCVPAERETLLDGRKLLDWIDHRRINLVHCVPSLFRLLLNEQLHPGR
                     LAHLKHVLLAGEALLPSDVRRWTEVYDDRIQLVNLLGPTETTLAKFCYFVRPSDAGRR
                     SIPIGKPIEGASAIVVDAKGNPCPPGMVGEIYIRTPYRSLGYYNRPDLTGEVFIKNPW
                     SNDPEDIVYKTGDMGRVLEDGNFEFLGRKDHQVKIRGFRVELGEIESLLRDHPSIRDV
                     VVVTGEEAGETFLCAYIVRNDDVEPGVLKQYLSEYLPIWMIPAAFIQLEELPHTLSGK
                     VDRRALPAPRRLSEKPDAELVAPRSATEEIVAGIWSKVLRLERLSVLDRFFDLGGHSL
                     IATQILARVREVLEVEMPLKDFFEAPTVAGVAAYVDTAKQVGRTIHVPAIERLPREVK
                     HTQGGERA"
     gene            complement(23014..24885)
                     /gene="asnB"
                     /locus_tag="AC16CY_09755"
     CDS             complement(23014..24885)
                     /gene="asnB"
                     /locus_tag="AC16CY_09755"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3354115.1"
                     /translation="MCGLTGFVNLDRHEGVDRAVLTRMTDRLVHRGPDSCGYFVEDNV
                     GLGFRRLSIIDLQTGDQPIYSEDRSLVLLCNGEIFNYAELRDRLRDRHVFRTNSDVEV
                     ILHLYEEHGVELLQELNGQFAFVLYDRRKRTLFLARDHFGVAPLFYTLADGVFVFGSE
                     IKAILEHPMVRREVDLTGLDQVLSFPGLVSPRTMFKGIESLKSGHYVTLEDSRVAVRR
                     YWDLDYPRMSDGIDERPERYYASRVDELLLQSVRYRLQADVPVGFYLSGGLDSSLIAA
                     LIKRASPEVTRHSFSIAFSDENRELSEKRFQQMMVESNQSIHHELMFDWSEIQQRLPG
                     MIYHCECPVRETYNTCSMALSRATKKNGITVVLTGEGSDETFGGYIGYRFDQAGLRAE
                     RKHDLDSVLEDELRERLWGDRDLFYEQHQYALRDVKQALYSAGVNECFEQFDCTSFEL
                     VDKEQLRGRHPLHQRSYLDYKLRLADHLLSDHGDRMALAHAVEARYPFLDINLVQFAR
                     EIPPGMKVNGLTEKYIIKKVAEGQVPREIIDREKFGFRAPGSPYLLQQNIEWVNDMLS
                     PGRIKRQGYFNPDTIERLKTQYSRPGSKINPHLESDWLLIVLTFGILLDTFDLPSRN"
     gene            complement(24888..29486)
                     /locus_tag="AC16CY_09760"
     CDS             complement(24888..29486)
                     /locus_tag="AC16CY_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIR3354116.1"
                     /translation="MSARNESAGMQEDVAGFRLSPQQDRLWRLQQIGDWQNRRSACTV
                     LVDGAFDEAIFADTVRHVVERHEILRTTFRCVSGVTVPVQVPDAGQSPVIRQYDLSDR
                     AASQTHEIEQIVTDQLNRRLDLVHGPLLEIAVIRLAPGRRTLFLTLPGLCADWRGLGN
                     LVRELARHYSARVRQDVLSDEPLQYADLAEWQNQLLETADTEAGRTYWKNHRPRPTGE
                     IALPFPKESIEEGGFHPKVIAVRIDSQLSADIERLSSRLEAPTSALLLAAWRVLLGRL
                     TGQAEIPIGTAFDGRNYEQLEGTIGLFARYLPLSCSVVDDDPFSEVLRRVRHDVEEIA
                     EWQEYFDCRDMLAAADADIEQAHYPLCFDFEEIPPVLSVGDDTTFTIDRLSACTDQFR
                     IKLSSARRQGALSIELHYDHRYFAEGDIERLASQLQTLLTSIAKQPSAPIGDLEIVGE
                     SERHRLLLEWNRPPEVAAVVPNGCVQEQFEAQVDRTPDAVAVASSATTLDYRELNARA
                     NQLARYLRRAGVGTDTLVGLCVERSPEMVVGILGILKAGGGYVPLDPGYPRQRLMFMI
                     EDAGVSLLVTQQLLRDRFSPPTGSTVCLDADWEAIARESRENLSEPGMPESLAYVIYT
                     SGSTGSPKGVMVSHRALAQYLSWSTREYRVSEGRGAPLHSPLGFDLTITSVFPQLLTG
                     RCVSVLPDDLPVDELGEALSRAGGWSLLKITPSHLALLNQQLSHDRVPGLANVIVIGG
                     EALHGETLRPWRTHAPNTRIVNEYGPTEATVGCCIYEVPGGDGAADGSGAVPIGRPTV
                     GTQIHLLNSRLRLMPTGAAAELYLGGSQLARGYLGRPDSTAAAFVPDAFGDSPGARLY
                     RTADLARYRADGNLEFLGRVDHQVKIRGFRIELGEIEAVLGTYRGVRDVAVLAREDTP
                     GDVRLVAYLGAEADVDVGELRAWLNERLPDYMIPATFLRLESLPLTRNGKVDREALPA
                     PDSLRRSAKTKIAPRTPREEILATIWSQVLRLDEVGVEDDFFDLGGHSLLALQILTRL
                     NAAFQVNLSYRDLFESPTVASLAARLETAASTGLGAETRAIERTSRQGVLPLSFAQER
                     LWFMHQVEPDNPFYSMVRIVRFNGALDLAAFEQTIAEIVRRHETLRTAFLAEGGQPRQ
                     CILEPGRFPAPIADLSSLTLAEREPEARRVLSDEMGRPFDLARAPLLRVKVLRLTPRE
                     HIVVFTMHHIVSDGWSIERMIAEFTHLYAAFSQGGLSPLRELGIQYADFASWQRHWLQ
                     GEVLEAHLSYWTKQLQGEPAPLLLPTRHPRPAAPTRRGATQSIRVSGDLYRDLAELGR
                     SEGVTSFMLLLAAFQTLLYRYSGQDDIVVGTPVSGRTRSETDPLIGFFVNTLVLRTDF
                     SGPPSFREVLARVRRVCLDAYSHESLPYQKLVEQLRPLRTSNRALFQVWFQLDEPAPE
                     RASLHGLEVSSFKIDNVATEFDLWMSISHRDGGLDVTVHYGVDLFDADTVDQMLRRFE
                     RILADVAARPDLSVSALSMAAESETQQLLNDFNAPVQG"
     gene            complement(29547..>35952)
                     /locus_tag="AC16CY_09765"
     CDS             complement(29547..>35952)
                     /locus_tag="AC16CY_09765"
                     /EC_number="2.3.1.-"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0034081 - polyketide synthase complex
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HIR3354117.1"
                     /translation="GDPCEISALTQAFHAGTDRKRFCAIGSVKTNIGHLDVAAGVAGL
                     IKTVLALENRRIPASLHFERPNTEIDFDSTPFFVNDKLAEWPEGPTPRRAGVSSFGIG
                     GTNAHLVLEEAPQLESAPGSRARQLVVLSAKSAAALARATSNLADHLRAGSETALADV
                     AYTLQVGRSVLPHRRMFVCENREDAIALLEKPNHKPVSSHTHDGTDPVVVFMFTGQGA
                     QRANMGAELYRTEATFRRHLDECCDAFRAHLGLDLRDLLYPAAERTEEAARQLDRTQF
                     TQPAMFAFEYALARLWISWGIKPQAMIGHSIGEYTAACLAGVFTLEDAVAIVARRGRL
                     MGEMPTGDMLSIPLSESELEPLLGDGLSLAAINGPRLCVASGPKAAVDVLEATLEKQG
                     CATQRLRTSHAFHSAMMDPIVEPFADLMRRVHLQPPAIPFISNLTGDWIQASDATDPS
                     YWARHLRQAVRFSQGVEQLLAEPNRILLEVGPGTTLRSLAAAHQAKHERTTILASLPH
                     VKDKESDLEHMLQSLGQLWMRGCRPDWPALYPGEKRRRVPLPTYPFERQRYWIAPGSS
                     TVEEKPAPRRDVTDWLFEPSWKRSTLRPGPPLEKETWLLFVDDSGVGRQMEQMLASQG
                     SDAICVSIGSGFERRSERTFAIDPGRRADYVRLFTELSKGAGVPSHMVHMWSVDRDAP
                     YHTSTELFEQRQQRGFYSLLYLVQALSEAEATSPMQLSVVTSQVQDVTGDEQLCPDKA
                     TVVSAAMTIGTEFPHIRRRCVDISLAGPDDVRTQARNVLAEATSNATDLVVAWRGNYR
                     WLQSFEPMPLAAGAEPPARLRERGTYLITGGLGGVGLALAEHLARTVRARLVLVGRTE
                     LPDRKKWDELAGSAGPADPVGRRIRSIRSMEANGAEVLVCQADVSDKAQMSDVLALAN
                     ARFGPVNGVVHAAGLPGGGVMLRQTPETVRRVFAAKVSGTQVLDELLRTQHLDFFVLF
                     SSQTAFLGLPGRSEYSAANRFLDVYARHRAKARDGFVTSINWDTWQDVGMAVQSRKDD
                     DGSTREVFPEFGLKNSQGVDIFSRILASDAAQVLVAIRGVEAAASDERKTAAPVQAAA
                     PDARGSRPSAHPRPELQTEYEAPRSDSERSLTRIWQDVLGIEPIGIHDNYYELGGDSV
                     GGIQVVAKANQAGLALSIKHILQHQTIAELASVASGASRIEAEQGVVTGPVLLTPIQH
                     WFFERQFAAPHHFNQSVLLEEVQPLDVARVRDVLTRLEAHHDALRARFTRDESGWRQI
                     VAKPGAEAVVRQVDLSTVPDGEVAAAIEAAAAQSQGALDLSRGPLMQATHFDLGAGRR
                     GRWLVCIHHLAVDNLSWRFLLEDFEAIYQALGRGENVTLPPKTVSFKDWSARLNEHAR
                     SETVARELPYWLSVGEAGVEPLPRDVPDGENTYGSKATVSSSLTASETQLLLDDARKA
                     WNARVDELLLTATTQAFCKWSGQSALLLELEGHGREEFIEGIDVSRTAGWFTTIYPVA
                     LQIRPGARPGDAIRTVKEQLRAVPNNGMNFGLLRYLSPESGPRLERLPHAEIVFLYLG
                     TADQAEPGSSLFTQAAESRGPAHAPANARPHLMELAAGVAADQLTVSLTFGGKHRRET
                     AQALVTGLLESLRVLIRQRPSAAAGKLTPADFPLADLDQRTLDTLLGDDSRIEDIYPL
                     SPLQQGLLFHSLYDRDSGGYFEQLSCTLEGDLDLEAFQRAWQQVVDRHPALRTTFRYE
                     NLDRPLQIVHGQVALPWVVEDWRSLAQTDQQRQLTSLLAADRDRGLALTEAPLMRVTL
                     ARLGERTFQFVWSYSHMLLDGWSGPLIIQEVMSLYEGFRRSEAIELPPARPFRDYIAW
                     HAGQSQTAAESYWRGVMAGFTVPTPVGGSLTAAESTATGRSYRRRPLPLPSSTTDALR
                     DYARRHHVTLNTVLQGAWGLVLACISGSDDVVFGVSVSGRPPQLEGVDSIVGLFINTL
                     PLRVRLNRDDGVVPWLTAIQAGQVRQRDYEYSSLVDVHGWSDVPRGKPLFESLLVFKN
                     HPVGTLKGESALVIRDLHADDASHYALTLDVTLRPAVLCRVNYDARRFDDGEISDILS
                     YLEAAITTLVSSGEAESRTVGDILVSTDASHRGKQKSLAKSASREKLSAIARGRAARH
                     RG"
BASE COUNT         6410 a        12015 c        11358 g         6169 t
ORIGIN      
        1 cgccggcgac atagatttcc ccaggcaggc ccgcaggcaa gagctcgccg tgctcgtcgc
       61 gaacgtagac ctgaaggtcc gggatcggcc gccccgccgg gctgtccacg tgcggcgtat
      121 cgagatccgc ctgcgtgatc acgcgatagg tcacgtgcac cgtcgtttcg gtgatcccgt
      181 acatgttgac caggcgcggg cactcaccgt ggcggctcat ccactgcgcc aggtgacgca
      241 cgtcgagcgc ctcacccccg aagacgacgt accggagcgc ggacatcgca ccatccagac
      301 ggcgcttgcc ctcggctgct acgagctggc ggaacgccga cggtgtctgg ttcaggacgg
      361 tgacgcgctc gcgcgccagc aggtcgatga attcatctgg cgcacgcgag accccgtacg
      421 gaacgaccag cagccgcccg ccgtagcaga gggcccccca cagctcccag acagagaaat
      481 cgaaggcata cgagtggaag agcgaccaga cgtcttgcgc cccgaactcg aaccagtcgt
      541 cggttgcggc gaacagacgg gccacgttgc gatgcgtgac cggtgtgcct ttcggcgtcc
      601 cggtcgagcc cgacgtgtag atcacgtacg caacgttgtc gacgctcatg cttacagccg
      661 ggaccgtcgc tcgttcccgg gagattgcct cccacgcgcc atcgaggctg agcacatggg
      721 ctttcgtctc ggggaggcct gctgagaggc gatcctcggt caccagcaca tcgatcctcg
      781 cgtcagccac catgaacgcg agccgctcgg gcgggtgtac aggatcgagc ggtacgtacg
      841 ccccaccggc cttgaggatg ccgagtatgc cgaccactaa ctcgatcgac cgctccatgt
      901 acagcccgac caggacctcc ggacccacgc ccaatgaccg caggtggtgt gccagctgat
      961 tcgcacgggt gctcagtgcg ccatacgtga tctggtgtcc gtccaggatt acggccaccc
     1021 gatccggagt ccgcacgact tgccgctcga accactcgtg caggcacatg tcggtggcgt
     1081 aatcgaccgt cgcgtcgttc cactcccgca gttgccgggc tcgctcgtcc gcactgagga
     1141 gcggtatctc cgaaatccgc cgcccggcgt cggtgaccat gccttccagc acggcgacga
     1201 aatgccccgc cattcgttcg attgtcgacg cgtcgaagag atcggtgttg tactcgagga
     1261 cgccgtagaa tccgcccggc gactcggcaa gattgagggt cagatcgaac ttcgccgtct
     1321 tctgctcgac ctcgaccggc gacatcgaga gcccggcgac ctccacacct cccgccggcg
     1381 cgttctggag cgcgaacatc acctggaaca gcggggtccg gctcaagtcc ctcgggacat
     1441 ccagctcgtc gagaagcttc tccagcggga gatgctggtg ggcgtacgca tccagggcgg
     1501 tatcccggac ctgcgtcacg atctcccgga cgcttgggtc gccggcgaga tccgcacgca
     1561 tgacgagcgt attgacgaag agtccgacga ggttttccgt ctcggttcga ctgcggccag
     1621 cgatgggcgt cccgaccagg atgtcgtcct gtcccgtgta acggaacagc agactgaaga
     1681 agcccgccag gagcgtcatg aacggcgtgg cgccctgcgt ggcgctcagg gcccgcaccg
     1741 cctcgctcaa ctccttcgag agcgtgaccg gccggctcgc gccgcggaac gactggacag
     1801 gcggacgcga gcggtcggtg agcagctcga gcgattgggg cgcccctgaa aggcggtttc
     1861 gccagtatcc gagctgctgg tcgagtgccc cggcatcgag ccactgccgt tgccagaccg
     1921 ccacgtcaac atactgaaca ttcagatcgg ccagcggcga ggtttcaccg cgggcgaacg
     1981 ccgcatagat cctggacagt tcgccaatca cgatccccat cgaccatccg tccgagacga
     2041 tgtggtgaat cgtcagcaag aggacgtgct cggactcggc gagcgttgcc accgtcgctc
     2101 gcatcaacgg gcccctggtc agatcgaacg atcgattggc ctcatcggca gcgaggcgca
     2161 gcgcctgggt cgttctctcg gcgtccggca gcgcgctcag atcgacgacc gcgagcgctc
     2221 caggtgttgc tgcaccgatg tcctgccgag gaccgtcttt accagtcgca aaccgggcgc
     2281 ggaggatcgc gtgccgtctg agaatctcgt ccacagcgtg cgacaacgca gcgaggtgga
     2341 gcggcccctt cagccgcacc gcagacggaa tgttgtaggc cgcactacca gagtccaacc
     2401 gatccaggaa ccagagccgc tcctgggcgt gtgagagtgg cagatcgaca tcccggccaa
     2461 ccggagacag cggtatctcc aactggtggt cgccgtctgt cagcaagcgc tcgacgtagc
     2521 gggcctgccc agccaccgtc ggctcgtcga agactgcgcg aagcgggtac tcgacgttga
     2581 agagcgcgcg aactctcgac accatacgag tcgcgagcag ggagtggccg cccaaccgga
     2641 agaaatcgtc ccggacaccg attgtctcga tgcccaacac ttctgaccag agggcagcga
     2701 gcgcgcgctc ggttggtgtg cgcggtggct cgaactcgtc gagttcggtg cgactctgat
     2761 cgggttcagg cagcgcccgc cgatcgacct ttccgttggg tgtgagcggc agcgcgtcca
     2821 gcgtgacgaa cctggccggc accatgtagt cgggaagact cgccctcagg aaatctctga
     2881 gggaaacaac gctgtccgcc gtcgcgagat cgcccaccac gtacgccacg agctgcacat
     2941 ccccggacac ctcacgcggc tcgacagcgc acgcctccac taagacatgc cgctccaaaa
     3001 cggcttctat ctctcccggc tcgatccggt gcccccgtac cttcacctgt cggtcagcgc
     3061 gaccgagatg ctcgagcgtc ccgttgggcc gccatcgcgc cagatcgccg gttcgataga
     3121 gacgtgcgcc ggcggcgccg ctcaagtggt ccgggacaaa cggcccggcg gtcagatcgg
     3181 gtcgttgcca gtagcccgcc gtcacgccgg cgcctccgat gtacagctcg ccagctactc
     3241 ccacgggcgc gggttcgagc tggcggtcca ggacgtacac ctgagtattc gcgatgggcc
     3301 gtccgaccgt aatcggcgcg ctgctcgcga cgggcccgca cgtcgaccag actgtcgtct
     3361 cggtcgggcc gtacatgttc cagagcgcgc cgacacaggc gttcagacga tcggccagcg
     3421 cggccggcag cggctctccg ccgcacagca cggtcagtcc gcgcgccccc ggccagttgg
     3481 cttcgaacag gagacgccat gtcgccggtg tcgcctgcat gagggtggcg cccgaccgcg
     3541 agatcgctgc ctccagttgc tggccgtcgc tcaccgtcgc tgccgccgcc acccacaccc
     3601 gcgcacccac tgtcaggggc aacaacagct cgagcacgga aatatcgaac gagtgcgtcg
     3661 tcactgcgag cagcaggtcg tcagccgcga gccctggcgt gcgtgccatg ctcgcgagaa
     3721 agttcgtcac gcacccatgc ggcacgcaca cgcctttcgg tcggcccgtg gatccggacg
     3781 tgtagatcac atacgccagc gccgtcgggc cgaccgccaa cgacagattc tccacggact
     3841 cggtctcgcc cgcggacgtc actgcgccga tatgcacgac ggccgtgccc tcgggcacca
     3901 tcgaggcgaa ggcctttccg gtcaccaccg cagccgcagc cgagtcttcg agcatcaacc
     3961 tgactcggtc ggtgggatac gcggggtcga cgggcacata cgcagctccc gccttgagca
     4021 caccaaggat cgccacgacg aggtctgccg accgttcgag ccagatcgcc actcgcacgc
     4081 cgggaccgac acccagatct ctcagccggc gcgccagacg gttcgcctgc gtgttgagtt
     4141 cccggtagct gagcgtcgtc gtctccgcaa cgacggccac cgcatcgggt gtccgtgcga
     4201 cctgcgcttc gaacacctcg ggaatcgtcg ccgacggagg cgcggtcgcc gtgtcgttcc
     4261 actcgacgag cagccgtgca cgttcgtcag cgttcagcag cggcaatgcc gagacgcgcg
     4321 cgtcggggtt cgcgacgacg cccgcgacca gtgcctggaa gtgccccgcc atctgctgga
     4381 tcgtggcggc atcaaacaga tccgggttgt attcgagcgc ccccacgatg cctttgcgcg
     4441 actcgaagag gctcaacgcc acgtcgaact tcgaggtccc ctgttcgacg acgaccggtg
     4501 ccagcgagac gtcccgaatg tgcagcgcag ccgcgggtac gttctgaagc gcgaacatca
     4561 cctggaacag cggcgtgcgg ctcaagtccc gttccacctt cagctcgtcc accagcctct
     4621 caaacggcac gtcctggtgc gcgtatgccc cgagcgcggt ctcccggacc tgtgccagca
     4681 acgcgcggaa cgacgggtca gaaccgagtt ccgcccgcag gaccagcgtg ttgacgaaca
     4741 gcccgatcat cccttccgtc tcggcacgcg tgcgtcccgc gatcggcgtc ccgacaagca
     4801 catccggctg gcccgcgtac cgatacagca acgtctggaa gaccgccagc agcgtcatga
     4861 acagcgtcgc gccctcccgc cgactgagcg ctcccagcgc cgccgccgtt tcggtcgaga
     4921 tctcgactgg cacgcttcca ccggcaaaac tctgaacggc cggccgcggt cgatccgtgg
     4981 gcagctcgag gacgcgcggc gcgcccgcca gccgtgtccg ccagtaggcg agctgcgcgt
     5041 ccatggcgcc gccggccagc cagtcgcgct gccagacggc gtagtcggcg tagtgaagcg
     5101 tgggttcctg caaccgcgag gggcgcccgt cgatgaattc ctcgtacagc gtgcccacct
     5161 cgccgaccag gagtccggcc gaccatccgt cggtcacgat gtgatgcgcg gagaacagca
     5221 ccatgtgatc cgcgtccgac agacgcacca cgatggcccg caccaggggc cccgttgcca
     5281 ggtcgaacgg ctgctgagcg gcttgcgtgg ccagccggct cgctgtctcg tgacgggcat
     5341 ccggcgcaag acgactcagg tccgccagct cgatcgagat cctcgagacc ggggtaatgc
     5401 gctgcacggg gcggccttcg ccgtctcgcc cgacgacagc gcgcaggccc tcgtgccgtc
     5461 gtatgacctc acggtacgcg gccgcgaacg cggcgaggtg tagcgggccg gtgagtcgca
     5521 gcgcactggg cacgacatac gcgctccctc ccggctcgag ctggtcgagg aaccacaggc
     5581 gctcctgggc gtatgaaagc ggcgcatccc cctcaccgtc gcgcctggcc atcggcggcg
     5641 cgactggcgt ggcctctcct tgatgcgctg cctcgatcac ccgggcgaaa gcggcgaccg
     5701 tcggctgctc gaacagcgtc cgcagcggta gatcgacgcc gaacagcgcc cgcacacgcg
     5761 cccccacctg ggtcgccagc agtgaatgcc ccccgagctc gaagaagtcg ctgtgcgcac
     5821 ccacctgcgg aacgcccagc acgctcgccc agacatcggc gaggatctct tccatcgggc
     5881 tccgtggcgc cacgtaggtc gactcgatcg cctgaggctc cggcgccggc agcgcccgtc
     5941 ggtcaacctt gccgttcggt gtcaggggca acgcggccaa cgtcacgaac gtcgtcggca
     6001 ccatgtaatc ggggagccgg tccttcagcg cctcgcgcag ggcgccgccg ctggccgcgg
     6061 cggctccctc ggccgccacg acataggcca cgagacggtc gtcacccggg gctgcggtgt
     6121 acacgacgac gaccgcgtct ccgatgccgg gctgctgtcg caggacctct tcgatctccc
     6181 ccagctcgat gcggaacccc cgcagcttca cctgatcgtc gatgcgtccc agaaactcca
     6241 gctcgccgtc ggaaccgtgg aagctgagat ctcccgtcct gtagaggcgc tccccgggca
     6301 gctcgctgaa cggatggggc acgaacttct cggcggtcaa ctccgggcgt ttcaggtagc
     6361 ctcgacccag gccagcccca ccgatgtaga gctcgcccgc gatacccact ggcgtcggct
     6421 gcatgcggcg atcgagcacg tagagctgcg tattgatgat cggccggcca atcgcgacgg
     6481 caccggaggt cggcgtgtct tcggacactt cgtagacgca gcacccgacg acggtctccg
     6541 tcggaccgta ctcgttgatg agtcgcgttc gcggcgcgtg ggtcctccag aagtcgatgt
     6601 cggtcgccgt cagggcctca ccgccgatga cgaaggcccg cgtgcctccc gccgcttgct
     6661 ccggagggat cgtcgcggca agcatcttca ggtgagccgg cgtgagcttc accagtccaa
     6721 agtcgctacc cgaggccagg gcctcaccga gcgcctcgac ctcgcgcgct tccggcagga
     6781 gcaccacgcg tcgtccggcg atgagcggtg agatcaggct cgtgatggtc gcatcgaatc
     6841 caagcgagga ttgcaccggg gccccactcc cctcggcgag gggatacgtc cgcgtgcacc
     6901 agtcaagata gttcgctacg ctcgcatgct cgatcatcgt ccccttgggc cgtccggtcg
     6961 accctgaggt gtacatcacg tacgccagat ggcgtggcgt cacgtcggtt cgacgattca
     7021 cggtgtaatc ggcgtcgtcc cgcccaccgt gatcgacgca ttccaccgtg gccgaaccgg
     7081 cagcaatctc ctcgcgcagg tgttcctgtg tcaggacaag ctcagcccca cagtcctcga
     7141 gcatgtactg cagacgctgt ttcgggtagt cgcgaggcaa tggtacgtac gcgccaccgg
     7201 ccttgaggac gcccaggatg gcggccaaca tctccgggcc tcgctccatg aacaggccca
     7261 ccagcgcgtt gggcttcact ccgagttccg ccagacgaac agccagccga ttggcccagc
     7321 gatccaatac cgaatacgtc accgaccggt cttcgaagac cagggccacc gagtcgggag
     7381 tcttagccgc ctgctcctcg aacatctgat gaacgcaacg cgggccagtg agcgacacct
     7441 gcgtgtcatt ccagtcgacc aggagttgct ggcgctccgc cgtggtcaac atcgggatgt
     7501 cgtcgatgcg cggattggcg tcgctcagca ggccgtcgag cagcatctcg aggtgcgcca
     7561 gcattcgccc gacggccgcc gcgtcgaaac ggctgccgtc gtagtcgatg cgcagcgaca
     7621 atcgctcgcc cgggccaacg accactatga gcggatagtt ggtacgcgac tcggccacat
     7681 cctcccgaac cgccagtccg cccgcatttc cgtccgagaa aggccgcacg gggtagtttt
     7741 cgaagacgaa cgcactttcg aagagcggag tcgccgacgg cacgccgctc cagcgttgaa
     7801 tgtcgacgag agacgcgtac tcgtactcgc gttgttcgac ctgctggcgc tgaatgctcg
     7861 tcaacaagga gagcacgggc acatctgcca acactcgcgc gcgcacgggc agcatcgtga
     7921 tgaacagacc gaccatcgat tcagaaccac taaggtcggc cggacgaccc gacgatgtgg
     7981 tgccgtagac gacgtcatcg gtgccgccat aacggctcag gaggatcgcc caggccccct
     8041 gcacgaccgt attgagcgtc aggttgtggc ggcgagcgaa cgcctgcagc gcggctgtcc
     8101 tggccgcgga gatctggacg cgttcgctgc cgaacgcttc tcgcgtcccg acagtcgcac
     8161 ggcgtcggtt gactccgagc ggcgtcgggg cctcgaaccc cttgagcctt tcacgccaga
     8221 aggtctcggc cgctgtcggg ttccgtgcca tgagccaggc gacgaagtcc ctgaaaggcc
     8281 gtggcggtgg cacttgtggc tcgacgccgc ggcgcagtgc gtcgtagaag acctgcactt
     8341 cgtgcagcac gacagcgacg ctccagccgt ccaggagcac gtgatggaac gtccagaaaa
     8401 acctgtaggc gtcctccgac agccggatga gcgccattcg catcagcggc gcgcgatcga
     8461 gtcgaagcgg tctctgccgc tcgtcagcga cgaaatcagc aagacgggat tggtgttccg
     8521 ccgcaggcac gtcgcgccag tcctcgttga tccacggcag gtccacgcgc gaccgcacca
     8581 gctgcagcgg ccccttctcg ccttccaacg caaacgccgt gcgcagcacg gcatgcctgt
     8641 cgaccacgag ctgccacgcg cgctggaacg cggccgcatc gagcttccct tcgatcgtgt
     8701 agccgagccg ttcgacatag acgccggact ccggcgagta cagagtgtgg aacagcaggc
     8761 cacgctggag cggcgtcagc ggatagatgt ccgcgaccct ccctcgcgta gccggcgatt
     8821 tcgacggacg cgtcactcag tacctccagc tgctcggatc cgcccccggg ggagccgttt
     8881 ccttgatgcg ctcgagaatc cggggcacgt acatctggac gtagcgtagt tggctgtagg
     8941 caattccgcg ttcgtggtcg ccgttccagc aatgctccgc gcggtccccg taatcgacga
     9001 cgccggcgta ggggggagtc gcggacttca gaaactgctc tgtcaggtac accgcgttgt
     9061 tcaggtagta gttgtccatg tctccgacgt agatatggag cttcccccgg agcttcgggc
     9121 cgagagtgga ccagtcgcgc cgcaggatcc aggaaaggtc gtagttctcc ttccagtagt
     9181 ccgccacact tctatcgatc gcccccgtgc gtttgtccca gatgggccgg ggatagccgt
     9241 cgttccccac gggggagtag acggcctgcc agatgtccca ttgaccgccc gagcggcccc
     9301 ggcttgcgag ggccagctcg agatggttca actgctcgag cgtgccgctc acatggccga
     9361 gatagttgcg gcgcccggga cggggtgtcc ggcggaacgg tccgtcgatc cagtaggcgt
     9421 tggcgtcctc gtagaggttc accacggtgt aagcccggaa atcgatcgga tcagggcagg
     9481 caacgaacgc gccgttgtac tggtccggat agaagacctg cgccgccatc gcctcccacc
     9541 cgccggtgga tccgccgtag aggaaccggg cccatccctc tccgatccct cgaaactgct
     9601 tctcgatgta aggaatcaac tcgtgggtga ttgcgtctcc gtacggtccg aggttcgccg
     9661 agttcacggc gtaggaagcg tcgtagtacg gattggcgtg ctggatctgc acgaccagca
     9721 tgcgcggaaa gcccgggctg atccaatcct tgtacagcgc gtacgccgcc tcctctcgca
     9781 cgcggttgta gcagtccacg ccgtaccggg cgttgtacac gcacggggcc ttctcgtcgg
     9841 gcggatcggt gcgccaacca tcgaagtctc tcgtgaagtg accgtgaaag acggccagcg
     9901 gatagcgaac ctccgggtgc tctgcgtagc cggccgggag cagcacgtga gctcccagat
     9961 agacatccgt gccccagaac tttgacaaca gcgtgctctt gatgcgcacg tgcttcaccc
    10021 acggcgtgtc tttcggcgga tcgagcggcg gaattgtctg atcgagggtc aactcgacca
    10081 cgtcgtcctt tgcaggatcc acgcgccgct cgatcggcag cgaatagaga ttgcccggct
    10141 tcctcgccca ctgctgcccc tctcccatgt ccggcggcag cgacaccgta tggccatcgg
    10201 cgcgtgtgta ggttcgatag cggttgagca gcgcctgcac gcgatagttc ccggggggca
    10261 gccctgcgag gccggacacg ggatacccga atgccgcccc gtcaaccgtt cgcctgctgc
    10321 ccggcatcca gctctctacg tccacaccaa atacctgggc agactgaggg ccgtcgttca
    10381 cctggaacct cggctcaccg gcggcagccg cggataccaa cacgagaagg cggccctgga
    10441 gcgcttcgcg ggccgcaccg tcaggcaaag tgatcgtgaa tttcagcgag gtctccgacg
    10501 cgacaacctg agacaagggc acgtcggggc ccgtcgcacg tccgcatccg gcagccaaag
    10561 cgatgcacgg caccgccgct cgaaggatcg ccggggctct cacgccgatt gatgtcctca
    10621 gcctaccgat cgtggtcgta cagttggtcg tagagtgcct ttgcgatgag ggtgtggccc
    10681 aacgcattag ggtgaagccg atcccaccct gcgatgacca gttggtcata tcccgctgct
    10741 tcgaaggaac cttccagtcg aatcacttcg aaccccgccc gcgtcgactc gtgctcgaca
    10801 atgtcgtaga tcagccgata cgtgctctcg tacctcttct cttccaccaa gtaagggacg
    10861 atcaccacca gcacccggaa tccatgctgg cgctgcagcc tgccgagttc tccaagggcg
    10921 tatcgcagct tgccgatcct ggcatgagac aagtagttcg cgacacggaa ccgggatgca
    10981 gcggaaccat tcgtctccat ttcagtcttc aggcggctca tgattgcgcc cagttccgcg
    11041 tcacctgtcg tgtcagcgat gtagtccctg ttgtcggcgg cgaaattggc ctcgaggttg
    11101 gcgcgcatgt tgtctcgacc gagcccggca gtgtccaggc gcgacctgac gaactggaag
    11161 agccggattc tgtagatcgg cgacccgtat ctgcgcgccc tcagaatgta ctcgacgttg
    11221 gtactggcga cttccacatc gttcacgaca taaccgacca cgacgacgtc tggcttgtac
    11281 tgaatgccga tgtccttcag cgtcgcgacc tcctgcagcg tatcgtagcc gccgagagcc
    11341 atattcagga cctcgacggc gcggcccttt agacgcacca tgtcgtctaa ctgtctcgca
    11401 aaagactcgt tgggtcccag ggagttgccg aacgccactg agtcgccgac gacgacgacg
    11461 cggctggtgc cggcgggctt ttgcagttcg tactccctgt ccctgaatcc gcgggaattg
    11521 atcctgacgt gggtgcccca tgcataccct tccgcaaatg gcgtcgcctc gtagattcgc
    11581 tccttgaccg acgacggccg cagaatcagc tctcgcccgt ttgccagttc tccgaaaggg
    11641 ttgtagtcga acacgcgcag gacggcctcg atgaccaaca acggcgtcac gatggacgcc
    11701 agaagaagaa cggcctcgcc tctccctgga accttcaccg agagccggat ggagtgacgc
    11761 ctgacgacca gatacgcccc gagcgccacc aacacgatct gcgccacggc gataccggca
    11821 agcatcgtgg aattggctat gtggtggtcg gacacgaagg ccgcttcgat gacccacttg
    11881 ttgaacagga cgcccgtcgc gatcgacagg cctcccaagc agagcatcgc cgtatgtcgt
    11941 tgtaccttca tgacgccggg gtggagtgac gatcgaacgg accggaacgg tcagtattcc
    12001 tggccctgga gcagtcggtc gagcgtggcc tgatcgaggt cggcgagagg gaaatccgag
    12061 ggagatgcgc cgcccgcatc tggagacagg cagtgcgcga tgatgccccg aagagcatcg
    12121 gcgataccgt cgaccagccc ctgcatcgtc gcgcgcgcgt gcacgttctc gctgtactgc
    12181 agcgagagct ggagccgacc gtcgacgatg atcccggaca cctccatcag atggcggggc
    12241 cgggctcgag ggctctgcgc tggaccgctg gacccggtcg caaagcggaa cggcgtgttc
    12301 gctgcgagcg tctggtcgaa ccgaccgagg tagttgaaga tcacttctgc cggcgcatcc
    12361 ccgagttggg cgatgcgggc cggctcgctg tagcgaagaa gcccgtagcc gatgccccgc
    12421 tgtggaatct gccgaagctg ctccttgatc gccgtgagtg cgctccccgg atcctctttc
    12481 gcaggcagct cgagccgcac ggggaagatc gtcgtgaacc acccgacggt ccgggacacg
    12541 tcgagatcat cgaacggcga ctcgcgcccg tgtccttcga gatcgacgac cagcgcccgc
    12601 gatcccgtcc acggggcaaa cgcctgcagc agcgcggtga gcaggacgtc gttgatctgg
    12661 gtgccgtagg cctcggggac ctcggtgagc agcgcgtgcg agtcctcctc ggtcagcgtg
    12721 gccgacacga ccgcccggga ggcgatcgtg ttgggtccgt ccgtgcggtc cacgggcagc
    12781 cgcggcgcct cgcctgtcac ctgctgctgc cagtacgcca cttcctcgtt cagcgctccg
    12841 ctctgagcgt actcgtacaa cttccgtgcc cagtgctgga gggccgtcgt cttccgcggc
    12901 agcgtcgccg acaccccccg gatcgcctgg tcgtaggcgg tcaacaggtc ttcgagcaga
    12961 atccgccacg acacgccgtc gacaaccagg tggtgagcga tgaggaatac acgatccggc
    13021 tcgtcgccgc atgcgaagtg acgcacgtgg aagagcggcc cacggccaag gtcgaagctc
    13081 gtgtgaagcg cttcgatctg gcgggtcatc tcggcggatc gctcggccgg agcgagggag
    13141 gacagatcca ccgtcgagaa cggagatcgt ccgtcaggcg tcatgctctc ctgatgccat
    13201 tctccgtccc gcagctcgaa gcggtggcgg agcgcgtcgt gatggaggaa cagtgacgcg
    13261 acgctttggc ccaggcatgc cggatccagc cgcgcgcggg cctcgagcaa cagggcctga
    13321 ttgaagtggt gaggctcggc cgggggagac tcgaagaacc accgttggat gggcgtcagg
    13381 gccacggctc cggtcaccgc cccctgctcc gccgccactt cgcccactcc cgccacggcg
    13441 gcgagctcgg cgattgtctg atgctcgaaa atctgccttg gcgtgagccg cagacccgct
    13501 tcgttcgcgc gggccacgat ctgaatcgcg aggatcgagt ccccgcccac gtggaagaag
    13561 ctgtcgtgca cgccgagctg gtccacccca agcacacgag accagatcgc cgccagcgtc
    13621 ttctccctgg ggtcgcgcgg cgcgatgaag gccgtctcca gctccggcct cgattgctcc
    13681 ggagccggaa gcgcacgacg gtcgatcttc ccgttcgggg tgaacggcca ggcatcgagc
    13741 gtcacgaacg ccgccggaat catgtactcg ggcagcgagg ctctgatgtg cctctgaagc
    13801 tcggtgacgc tcaggggctg gtccccgctc gccagcatgt aggccaccag tcgcgtgtgg
    13861 cccggcgcgt cttccctcgc cacgaccacg gcatcccgga tcgactggtg ctggcccagc
    13921 accgtctcca cctcacccag ctcgatccgg tggcctcgga tcttcacttg atggtcgatc
    13981 cggccgagga actcgatccg accgtccggc cgataccgca cgcggtctcc ggtgcgatag
    14041 agtcgcgatc ctccggccac atcgaaagga ttcgggacga accgcgccgc tgtctcgtcc
    14101 gggcgtttac ggtacccgcg tgagacgcca gccccgccga tgtgaagctc gccgggcacg
    14161 ccgatcggga gcggtctggt ttcgcgatcg agcacgtaca ccttggtgtt ggcgatcgga
    14221 tgcccgatcg aggtcggcgc gtcacggtcc tctacgtggt cgagcgtcga ccagaccgtg
    14281 gtctccgtcg gaccgtacat gttccagacc gatcgagcgc cggacagcag ccggctcgca
    14341 agatcctgcg agagagcctc accgccgcag agcgccttca gtgacgcgcc gcccgaccac
    14401 ccgttgtcaa gcaccaggcg ccatgtcgcc ggagttccct gcatggccgt ggcctggtgg
    14461 cgttcgacga gcgcagccag ctgcccgccg tcgcgcacgt catcgggacc tgcgacgacc
    14521 agtcgggccc cggtggtgag cggcagaagc agttcgagca cggagatatc gaacgaaatc
    14581 gtcgtgaccg acacgaatgt gtcgtcgcga tcgagtccgg ggaccgcacg catggtttcg
    14641 aggaagttca cgacggaacc gtgcgcgatc tgcacgccct tcgggcggcc ggtcgaaccg
    14701 gacgtgaaca tcacgtacgc caggttgcgg ggatcgagac tgtgagaggc actgcagtct
    14761 gccgtcggcg cgttggccgg ccaatcggcg tcgatgcaaa ggacctgtac gtctgctcca
    14821 aaccggtgtg ccaggtgggc ctgtgtgaga acgagcgcca cgtcggcatc ctcgaggatc
    14881 actcgaagac gctgctcggg atacgacggg tcgagagcaa cgtaagcgcc gcccgcgttg
    14941 aggatcccga gcattcccac gacgagatcg agggaccgat ccaaacacag tccgacgcgc
    15001 acctccggcc cgacgccgac actctgcaga taactcgcca gccgggccgc ccggtcgtcg
    15061 agctgccggt acgtcagttg ccgcccctcg aacacgaccg cgacagcgtc cggcgtcagg
    15121 gcgacctgtg cctggaacac atcgggaatg cacgcatcgc tccggtacat ctcctccgtc
    15181 gcgttccagg tctccagcaa ctgcgtgcgt tctgccgttg agagcagctc cagcgtggcg
    15241 accggacgcg tgggctcggc gacagccgcc tcgagcagct gggtgaagtg cccgaccatc
    15301 cgccggatcg tcgccgcgtc gaacaaatcc gagctgtatt ccaacgtccc ccgaattccc
    15361 gatggcgatt ccgccaggct cagggtcatg tcgaatttcg acgtcgcttg ctcgatttct
    15421 accggcaaca aggacaggcc gggaagcgac agggtttcgg tgggcgtgtt ctgcagcgcg
    15481 aacatgacct gaaacagcgg cgtgcgactc aagtcccgtt ccacttccag ctcgtccacc
    15541 agtttctcga acggcaggtc ctgatgcgcg taggccccca acgctgtttc gcgtacctgg
    15601 gccagcacgt cccggaacgt cagtgctcca tcgaagtctg cgcgcatgac cagtgtgttg
    15661 acgaacagcc caatcagccc ctcggcttcc gcgcgcgtgc gcccggcgat cggcgtcccg
    15721 acgaggaccg tcttctgacc cgagtacttg tagagcagcg tctggaaggc ggccagcagc
    15781 gtcatgaacg gcgtcacgcc ttcgcgacgg ctcaacacct gaagtgcttc ggatagatcc
    15841 ttcgcgatcc tgaccggctc gcttgcaccg gcgaggccct ggagtggtgg cctgggcctg
    15901 tcggtcggca gctcgagcaa cccggacgca cccgccagtc gatcgctcca gtaagagacc
    15961 tgcgcgtcca tcgcgccggc gtccagccag ttccgctgcc agactgcgta gtccgcatac
    16021 tgaatgggcg gctccgcaag tggcgagggg tggccggtga tgaacgctgg atacagcgtc
    16081 gtgagctccc gcacgatgag tcccatcgac cacccgtcgg tgacgatgtg gtgcgccgag
    16141 aacagcgcga cgtgggtctc gtcctcgagc cgaagcacgg tcgcccgcac cagcggcccg
    16201 gacgccatgt cgaatgggcg cctggccatg agcagggcca gccggcgcat ttcctcttcg
    16261 cgcgcaatcg gggcgagaga gctgagatcg acgaactcga gggcgatcga ctcgccatcg
    16321 gtgatgcgct gttcgccgcg cccgtcaccc gttgtgacga tcaccgtccg caaggattca
    16381 tggcgccgga cgacttcgcg gtaggcagcc gcgaacgagg ccacatggag cgttcccgtc
    16441 agccgcagcg cgctgggtac gacatacgcg ctcccggagg gctcgagctg gtcgaggaac
    16501 cacagacgtt cctgggcgaa agagagcgga gcgctgccgc ggtcgacgcg acggtgtatc
    16561 gatggagcgc gaacgaccgc gcctccttgt ttcgccgctt ccacctgcgc agccagtcct
    16621 gcgaccgtcg ggtgctcgaa gagcgtgcgc agcgggagat cgacttcgaa cacctgtgcg
    16681 acgcggctca tcacccgggt caccagcagc gaatgcccgc cgagctcgaa gaagtcgctt
    16741 tgcacgccga cctgcggcac gcccagcacg ctcgcccaca cgccggcgag gatctcttcc
    16801 accgggctcc gcggcgccac gtgggccgat tcgagcgcct gggtctccgg cgccgggagg
    16861 gcccggcggt cgaccttgcc gttcgacgtg aggggcaggg catccagcgc gacgaacgcc
    16921 gccggcacca tgtagtcggg caacgacgtc ttcagaaaat ctctgagctc ggtcactccc
    16981 ggcggttccg cctctgccac cgtgtaggcg acgagtctca cctgacccgg cgtgtcctca
    17041 cgcgcgacga cggccaccgc ccgcaacgcc gggtgtcggc tcagggcccc ttcgatctcc
    17101 cccaactcaa tgcggaagcc tcgaatcttc acctgatgat cccgccgccc gagatactcc
    17161 atttcgccgt tcggcaggta aagccccagg tctcccgatt tgtacaggcg tgctccggcc
    17221 gggccgccaa agggatgtgg aatgaaccgt tccgccgtga gctccggccg attgagatag
    17281 ttccgagcca gtcccgctcc gcctacgtgc agctcgccag gcacaccgac cggcagcagt
    17341 ctcatacccg aatcaacgag atacgtctgc agagtaggaa tcggcacgcc gatgttgctg
    17401 ccgtcctttt cgagatcctc agcctcgaga cccttgaacg tcacatgaac ggttgtttca
    17461 gtaatcccgt acatgttgac gagcttcacc tctgggtatg cccggttcca cggctgtaac
    17521 tgccttgggg ccagtgcctc gccgccgaaa atgatgtagc gcagggccac gctcgtcctt
    17581 cgctggctca gcgcgtggtg catgagctgc tggaaggcgc tcggtgtctg gttgagaacc
    17641 gtcacctcct cggacagtat gagacccaga aactgctgcg gatccttgtt gacgccttcc
    17701 gagacgatga ccagtctgcc tccatagagc agggcgccgt acatctccca gacggagaag
    17761 tcgaaacagt aggagtggaa caccgaccac acgtcttctt ccgtgaacgc aaactgcatg
    17821 tggtcgttga ccatcagccg gaccacctgc cgatgctcga gcagaacgcc cttgggtctt
    17881 ccggtcgacc ccgatgtgta gatgcagtac gcgatgttgt cgggaacggc atgagccgct
    17941 ggtggctggg aagatctgct gcctattgcc tcgcgatcgc ggtcgagcag gatcagtgag
    18001 gaaacgctgt cggccaggat accgatactg ctggatgtcg aaatggcatg cgagacaccg
    18061 gaatcgttga cgatgtagga gcgccgttcg tccggataat ccgggtcaat cggcagatac
    18121 gccccgccgg ccttcagaac gcccagtatc gccacgatca tgtccggaga tctggccaga
    18181 cacagggcca ccagtgtttc ccgaccgacg ccctgttcgc gaaggtaatt cgcgagccgg
    18241 ttgctctcct cgtccagctg gcggtaggtc aactgcgtgt gctggaagac gaccgccact
    18301 cgatcgggat accgggcaac ctgctcctcg aagatcgagt gaatggtctt ctcgcgcgga
    18361 tactcggcgg ccgttcgatt ccaatcgacg agctgagttc gtttttcggc ctggctgagc
    18421 agatcgacgt cgttcaccgg aacatcggaa ccggacgcga agcactcgag cagcgcttca
    18481 aagtgcccgg ccagctgctc gatcatgaac tcgtcgaaca ggtcggtgtt atagacccac
    18541 gtcccgacga acccctcggc cgtacgctga actgacaggt tgacgtcgta cttgccgtga
    18601 ccgagattcg tatcgaccag aatggctttc gtctgtccga ccgtgattgc ggcgggaggg
    18661 gcgtcgtcga atcgaaagag cacgtcgaac agcgccgtcc ggctcatgtc gacctgggga
    18721 ttcagctcct tgacaagcca atcgaagggc atgtcttcat gccgccgcgc atcgtcgaca
    18781 gtccggatga tttgcttcag gaacgcgccg aaggtcgggc ggccaccgag attgctgcgc
    18841 aggaccaaca ggtttgcgaa gggcccgacg gcgtttcgaa cggcagcatg acgacgacag
    18901 gggacactgg taccgacgac gatctcctcg tgacccgaat agcggtgcag gagcacattg
    18961 aatgcggcca gcaggacctc gaacgttccc gtgtgctcag cacggccacg cgcgtctatc
    19021 tggctggaca ggcggccgct gaacgcgaag gtccgccgac cgaccgaata ggtatgaacg
    19081 gccgggcggc cgacgcgcgt tggcagtgtc agtgcctgaa gtgggccggc cagctggcgc
    19141 ttccaataga acagcagttg ctcgacaacg tcttccggca aagtgcgctg ccaattcgca
    19201 tggtcgatgt acctgacggt caggtccgcg aaccgggcag gccgcttttc ggtctgagcg
    19261 ttataagact ctgcgatctc ctgtgcgagc acgcgtaacg accacgtgtc ggccgcacta
    19321 tggtgcacgg tcacgaccaa tacagcggtg gcgtcggcaa ggcgaaagag cgtggcccga
    19381 atcagtcggt cacgatccag cgcaatcgga atctgcgacg cctccaacgc gaactggacc
    19441 accaactcgg acgcatctcg gtccgggtct cctgcgacgt cgcgaacgtt cagttcgaga
    19501 tcggtatgcg gaacgacttc ctgatacgcg tgaccgtcgc gcgtgacgaa tcgcgtgcgc
    19561 aggacgtcgt gtcggccagt gacgagattg atggcccgct gcagcaggtc cgcatcgacg
    19621 cctccctgaa aatgcaggac cagcggcagg ttgtgataga ccggatgcga ttcatagaca
    19681 ttgccggtct cgaactggtc gataaaccac attcgctcct ggtggtagga cagcgaacct
    19741 cgggagtcgt gggacgctga aagcggaatg ctcgccagct cgagatccgg atcgagtcgc
    19801 agggcgtcga tcggcgccgc ggggtcgcga acaatactct cgaggagcgt gataaaccgg
    19861 cggcacaggc gcgcgatcgt tggcgcctcg aacagatcgg tgcggtacac gaacgtgcca
    19921 cgaatgccgt ctccagactc ccacgcgtac agatccaggt ctgatctgac gtgcgcgctc
    19981 gtgtcatcca gtgcagacgc cttgaggccg gcgaggtcaa tcgccatctg cggggtgttc
    20041 tgaaagatga acatcgcctg aaacaacggc atctttccga gatcccggat cggctcgagc
    20101 tcctcgacca gcttctcgaa aggcagatcc tggttggcat acgccccgag cgcagccacc
    20161 cgcacctccg ccagtaattc ctggaccgac atgtcatggc gcacgtcggc ccgcagcacc
    20221 aacgtgttca cgaacaaccc gatcaaccct tcagtttcgg tccgggtacg cccggcgatc
    20281 ggcgtcccga cgaggacctt ctcctggtcc gcgtaccgat ggagcaacgc ctggaaggcg
    20341 gcgagcagcg tcatgaagag cgtcgcaccc tcccggcggc tcagcgccac cagcgcctcc
    20401 gacaacgcgc gcgggagcca gaccggctcg gtcgcgcccg cagtgctctg taccgccggc
    20461 ctcggtcgat ccgcgggcaa ctccagcaac cgtggagcac cgcgcagtcg atccctccag
    20521 tacgagagct gggcctctag gacatcaccc tggagccatt gccgctgcca gtgggcgtag
    20581 tccgcatact ggatgggcag ctccgggaag gccgacggac gtccccgcga gaaggtctcg
    20641 cacacgctgc tcagctcact gagcaacacg cggatcgacc acacgtcgga gacaatgtgg
    20701 tgcatcgtca gaagcagcgc atgctcctgg tccgccaggc ggatcagcgt cgcccgcatc
    20761 agcggacccc gcgacaggtc gaaggggcga gcggcctcct ggcgcgccag acgtcggacc
    20821 tccttttccc ggccggtttc cggcagctcg ctcacgtcga cccgacagag cgcgcacggc
    20881 tcccctgcgt cgatgacctg ctgtgcccga ccctcggact cggtgaacgt cgtccgcaac
    20941 gcctcgtgcc gacgaacgac ctctcgaagc gccccgttca gcgcttcggc tcgcagtgga
    21001 cccgacagcc gtatggccgc cggaatgttg tacgcagcgc tccccggctc cagtcgatcc
    21061 agaaaccaca ggcgttcctg agcgaaggaa gctgcaaatc tcacgctcgc tctcctccct
    21121 gcgtgtgctt cacttcgcgc ggcaagcgtt caattgcggg tacatgaatg gtgcgtccaa
    21181 cctgcttcgc ggtgtcgacg taggcggcga cccccgccac cgtgggcgcc tcgaagaagt
    21241 ccttgagcgg catctctacc tcgagcacct cacgcacgcg cgcaaggatc tgcgttgcga
    21301 taagggagtg cccacccaga tcgaaaaacc tgtcgagcac actcagccgc tccaggcgca
    21361 gcacctttga ccagatgccg gcaacgatct cttcagtcgc ggatctcggt gcgacgagct
    21421 cagcgtcggg cttctccgag agcctcctgg gcgccggaag cgcccgcctg tcgaccttcc
    21481 cgctgagggt gtgcggcagc tcctccagct ggatgaaggc cgcagggatc atccagatgg
    21541 gcaggtattc ggacaggtac tgtttgagca cgccgggctc gacgtcatcg ttgcggacga
    21601 tgtacgcaca cagaaacgtc tcgcccgcct cttctccggt gaccacgaca acgtctctga
    21661 tggacgggtg gtcgcgcagg aggctctcga tttctcccag ttcgacgcgg aacccgcgaa
    21721 tcttgacctg gtgatctttc cttccgagaa actcgaagtt cccatcctcc aggacacgtc
    21781 ccatgtcgcc ggtcttgtag acgatgtcct ccggatcgtt actccatgga ttcttgatga
    21841 acacctcgcc ggtcagatcg ggacggttgt aatagcccag cgagcggtac ggtgtgcgta
    21901 tgtagatctc tcccaccatt ccgggtggac acggattccc cttcgcatcg acgacgatcg
    21961 ccgacgctcc ttcgatcggc ttcccgatgg gaatcgagcg cctgcccgcg tcggacggcc
    22021 gtacgaaata gcagaacttc gccagcgtgg tctcggtggg gccaagcagg ttgaccagct
    22081 ggatccggtc gtcgtacacc tcggtccatc gcctcacgtc cgagggaagc agcgcctcac
    22141 cggccagcag cacgtgcttc aggtgcgcga gccggcccgg gtgcagttgc tcgttgagaa
    22201 gcaggcggaa cagcgacggc acacagtgca cgagattgat ccgccgatgg tcgatccagt
    22261 cgagcagctt tcgcccatcg agcagcgttt cgcgctcggc agggacgcac accgtaccgc
    22321 cggcacacag tggcacgaag acgtccctca gaaacggatc gaatgacggc gtcgtcagct
    22381 gactgacccg cgtgccgtga ccagcgccga cggtcttgat ctcccacccg atgaagtgat
    22441 cgagtccctt gcgacgcccc gcgatcccct tcggccggcc cgtggagccc gacgtgaagt
    22501 agacgtagca catgtcgtct ggcgcgactt cgatcggcag cctcgcgtcg cccgggctct
    22561 cgtcctcgag gtggatgacg ccatgagctc gttcgcacgc ctcgtcgagg cacacgaccc
    22621 tcgacccggg gtgacggtcg ttcagcgcct cgcgaacatg cgcgcccgtt ccgcaagcca
    22681 acagcgacca cgccggcgca agctccgaca cgattccctc gagccgtttc ggcggcatcg
    22741 tcgagtccat cggggcgaac gcaaggccgg ccttcagcga cgcgagcatc ccgacgatca
    22801 cctgcacacg gtcctcgacg aggatggcga cgaccgaccc tttcgtggca ccggcggaga
    22861 ggaaaagcct ggcgagccgg tccgccttcc ggcgaagctc gccgtaggtc agcctccgtt
    22921 cgccgcactc gatcgccgtc gtctcactga aagactcggc ggcgcggtcg aaccaatcaa
    22981 cgatgccttc gcgtgtcatg taatgctgtt acctcagttg cggctgggca gatcgaacgt
    23041 atcgagcaga atgccgaacg tgagcacgat cagcagccag tcgctctcca ggtggggatt
    23101 gatcttggac ccgggccgtg aatactgcgt cttcagcctc tcgatcgtgt ccgggttgaa
    23161 gtacccctgc ctcttgatcc ggccgggcga cagcatgtcg ttcacccact cgatgttctg
    23221 ctggagcaga tacgggctgc ccggcgcacg gaacccgaac ttttccctgt cgatgatctc
    23281 tcgcgggacc tgaccttcgg cgactttctt gatgatgtac ttctcggtca ggccgttgac
    23341 cttcatgcct ggcgggatct cccgcgcgaa ctgcaccagg ttgatgtcca gaaagggata
    23401 tctcgcctcg accgcgtgtg cgagcgccat cctgtccccg tgatcggaca gcaggtgatc
    23461 ggcaagcctc agcttgtaat cgaggtacga acgctggtgc aacgggtggc ggccccggag
    23521 ttgttccttg tccacgagct cgaagctcgt gcagtcgaac tgctcgaagc actcgttgac
    23581 accggcggag tacagggcct gcttcacgtc cctcagcgcg tactgatgct gttcgtagaa
    23641 gaggtcgcga tcgccccaca gcctctcgcg caactcgtcc tcgaggacgg agtcgaggtc
    23701 gtgcttcctc tccgcgcgca gccctgcctg gtcgaagcgg tagccgatgt agccgccgaa
    23761 cgtctcgtcg ctaccttctc ccgtgagcac gacggtgatg ccgttcttct tggtcgcacg
    23821 cgacagcgcc atcgagcagg tgttgtacgt ctccctgacg gggcattcgc agtggtagat
    23881 cattcccggc aggcgctgct ggatctcgga ccagtcgaac atcagctcgt ggtgaatcga
    23941 ctggttggac tccaccatca tctgttgaaa gcgcttctcg gacagttccc ggttttcgtc
    24001 gctgaacgca atcgagaacg aatgacgcgt cacctccggt gaggcccgtt tgatcagcgc
    24061 tgcgatcagc gacgagtcga gtccgccgct cagatagaac cccaccggca cgtcggcctg
    24121 caggcggtat cggaccgact gcaggagcag ttcatcgacg cggctcgcat agtaacgttc
    24181 cggcctctcg tcgatgccgt cgctcatcct gggatagtcc agatcccagt agcgtctcac
    24241 cgcgacgcgt gaatcctcga gggtgacata gtggccgctc ttcaggctct cgataccctt
    24301 gaacatcgtc cgcgggctga ccaggccagg gaacgagagg acctggtcga ggcccgtcag
    24361 gtccacttca cggcggacca tcggatgctc caggatggcc ttgatctccg agccgaacac
    24421 gaagacgcca tccgcgagcg tgtagaacag aggagcaacg ccgaaatggt ccctcgcgag
    24481 gaagagcgtc cgctttcgcc gatcgtacag gacgaaggcg aactggccgt tcagttcctg
    24541 caacaactcg acgccgtgtt cctcgtacag gtggaggatc acctccacgt cgctgttggt
    24601 gcggaagacg tggcgatcgc gaaggcgatc ccgcaactcg gcgtaattga atatctcgcc
    24661 gttgcacaac agcacgagcg accgatcctc gctgtagatc ggctgatcgc cggtctgcag
    24721 gtcgatgatg ctgagccttc gaaatccgag ccctacgttg tcctcgacga agtatccaca
    24781 cgagtcgggg ccgcgatgca cgaggcggtc ggtcatcctc gtgagcaccg cacggtcgac
    24841 accctcgtgc cgatccaggt tgacgaaacc ggtgagcccg cacatggcta cccctgcacc
    24901 ggcgcgttga agtcgttgag cagttgctgg gtctccgact cggcggccat cgacagagcg
    24961 gacacgctca ggtcgggacg ggcggccacg tcggcgagaa tgcgttcgaa gcggcgcagc
    25021 atctgatcga cggtgtcggc gtcgaacagg tcgactccgt agtgcaccgt gacgtcaagg
    25081 cctccgtctc tatgggagat gctcatccat agatcgaact ccgtggcgac gttgtcgatc
    25141 ttgaagctgc tgacctcgag tccgtggagc gacgcccgct ccggggccgg ttcatccagc
    25201 tgaaaccaga cctggaacag cgcgcgattc gatgtccgca gtggacgcag ctgttccacc
    25261 agcttctgat atggaagcga ctcgtgactg taggcgtcga ggcaaaccct ccgcacacgc
    25321 gccaggacct cgcgaaagga gggcgggccg gagaagtccg ttcgcaggac gagggtattc
    25381 acgaagaaac caatgagcgg atcggtctcg gatcgcgtcc ggcccgagac gggcgtgccg
    25441 acgacgatgt cgtcctgacc gctgtagcgg tacagcagcg tctgaaacgc cgcaagaagg
    25501 agcatgaagg aggtcacacc ttcgctccgg cccagctcgg ccaggtcccg atacaggtcg
    25561 ccggacactc tgattgactg ggtggcgccg cggcgggtcg gcgccgccgg tcgaggatgc
    25621 cgcgtcggga gaagcagggg cgccggctcc ccctggagct gtttcgtcca gtaagacagg
    25681 tgcgcctcca ggacttctcc ctggagccag tgtcgttgcc aactggcgaa gtcggcgtat
    25741 tgaatgccca gctcgcgcag cggcgagaga ccaccctggc tgaacgcggc gtagaggtga
    25801 gtgaattcgg cgatcatccg ctcgatcgac cagccgtccg acacgatgtg atgcatcgtg
    25861 aacacgacga tgtgttcccg cggagtcaac cggaggactt tcacgcggag taatggcgct
    25921 cgagccaggt cgaaggggcg tcccatttcg tccgacagga cgcgccgtgc ctcgggttcg
    25981 cgctcggcga gcgtcaggct tgacagatcc gcgatcggag ccgggaatcg acctggttcc
    26041 agaatgcact gacgcggttg gccgccctcc gcgaggaacg cggtgcggag cgtttcgtgc
    26101 cgcctgacga tctcggcgat cgtctgctcg aatgcggcga ggtccaacgc gccattgaaa
    26161 cggacgatcc tgaccatgct gtagaacgga ttgtctggtt cgacctggtg catgaaccag
    26221 agacgttcct gtgcgaacga gagaggcaga accccttgcc ggctggtacg ctcgatagcc
    26281 cgcgtctctg cacccagtcc agtgctggcc gcagtttcga gacgcgccgc caggctggcc
    26341 accgtgggag actcgaacag atcgcggtac gacaggttca cctggaatgc cgcgttcagc
    26401 cgggtcagga tctggagcgc caggagagaa tggcccccca ggtcgaagaa atcgtcttcc
    26461 acgccgacct cgtcgaggcg cagcacctgc gaccagatgg ttgccagaat ctcttcgcgc
    26521 ggagtacgtg gcgcaatctt tgtcttcgcc gatcgcctca gcgaatcagg cgcaggcagt
    26581 gcctctcgat cgacctttcc gttgcgcgtc aacggcaggg actccagcct gaggaacgtg
    26641 gccggaatca tgtagtctgg caggcgctcg ttcagccacg ctcgcagctc gccgacgtcg
    26701 acatccgctt cggcacccag atacgcgacg aggcgcacat cgcctggcgt gtcctcgcgg
    26761 gcaaggacgg ccacgtcccg cacgccccgg tacgtgccga gcacggcctc gatctcgccg
    26821 agctcgatgc gaaagcctcg aatcttgacc tgatggtcga cccgtcccag gaactcgagg
    26881 tttccgtcgg cgcggtaccg cgccaggtcg gcggtccggt acagtcgcgc accggggctg
    26941 tcgccaaacg catcgggaac aaaggccgcg gctgtcgagt ccggacgccc aaggtagcct
    27001 cgcgcaagct gtgagcctcc gaggtacagc tccgcggcgg cgcccgttgg catcagcctc
    27061 aaccggctgt tcagcaggtg gatttgcgta ccgaccgtcg gtcgaccgat gggcaccgcc
    27121 cccgagccgt ctgcagcgcc gtctccgccg ggcacttcgt agatgcagca gcccacggtg
    27181 gcttcagtgg gaccgtactc gttgacaatc ctggtgttgg gcgcgtgtgt cctccagggg
    27241 cggagagtct cgccatgcaa cgcctcgccg ccaatgacga tcacgttggc caatccggga
    27301 acccggtcgt gagacaactg ctgattcaag agtgccaggt gggatggcgt gatcttcagc
    27361 aggctccagc cgcccgcccg actgagtgcc tcaccaagct cgtccacggg taagtcgtcc
    27421 ggcagcaccg acacgcaccg gccggtgagt aattgaggga acacactcgt aatcgtcagg
    27481 tcgaagccga ggggggaatg tagcggggca ccacgcccct ctgacacccg gtattcccgg
    27541 gtgctccagc tcaagtactg cgcgagagcc cgatgggaga ccatcacccc cttgggcgac
    27601 ccggtcgatc cagatgtgta gatgacgtac gcaaggctct cgggcattcc cggctctgac
    27661 aggttctcgc gactctcgcg ggcaatcgcc tcccagtccg cgtccagaca aaccgtcgaa
    27721 cccgtcggcg gtgaaaaccg gtcgcgcagc agctgctgtg tcactaacag ggataccccc
    27781 gcgtcctcga tcatgaacat caaccgctgt cgcgggtaac cgggatcgag tgggacgtac
    27841 cctccgccgg ccttgagaat gccaagtatc cctacgacca tctcgggcga gcgctccacg
    27901 cacaggccaa cgagggtgtc cgtgccgacg ccggccctcc tcaagtagcg agccagttgg
    27961 ttcgcgcggg cattcagttc ccggtagtcg agcgtcgttg cactcgacgc cacggccact
    28021 gcgtctggag tgcggtcgac ttgtgcctcg aactgttcct gcacgcagcc gttcgggaca
    28081 acagcggcaa cctcgggcgg gcggttccac tcgagcagca gcctgtgacg ctcggattcg
    28141 cccacgattt ccagatcgcc gatcggcgcg ctcggctgct tcgcgatgct ggtcaggagc
    28201 gtctgaagct gcgacgccag ccgctcgatg tcgccctcgg caaagtagcg atggtcgtag
    28261 tgcagctcga tcgagagcgc tccctgccgc cgcgcggagg acaacttgat ccggaactgg
    28321 tcggtacacg cgctcaggcg gtcgatggtg aacgtcgtat cgtcaccgac cgacaggact
    28381 ggcgggatct cttcgaagtc gaaacagaga gggtaatgcg cctgctcgat gtcggcatcg
    28441 gccgcagcga gcatgtccct gcaatcgaaa tactcctgcc actccgcgat ctcttcgaca
    28501 tcgtgtctga cgcgtcgaag aacctcgctg aacgggtcgt cgtcgacgac ggaacagctg
    28561 agcgggaggt atcgtgcgaa caggccgatg gtaccctcga gctgctcgta gttccgcccg
    28621 tcgaaggctg tgccgatggg gatttccgcc tgcccggtca atctccccag caacactcgc
    28681 catgctgcga gcagcagtgc cgaggtcggc gcttccagtc ggctgctgag ccgctcgatg
    28741 tctgcgctca actgcgaatc gatccgaact gcgatcacct tcgggtgaaa gcctccttcc
    28801 tctatcgact ccttcggaaa gggcaacgcg atctcgccgg tcggccgagg cctgtgattc
    28861 ttccagtacg tcctgccggc ttcagtgtca gcggtctcga ggagttgatt ctgccattcg
    28921 gccaggtccg cgtactgcag gggttcgtcg gacagtacat cctggcgcac tcgggcagag
    28981 tagtgccggg ccagttcacg tacgaggttg ccaagacccc tccagtccgc acacaggccc
    29041 gggagagtca ggaacaacgt gcggcgacca ggcgccaggc ggatcacggc aatctcgagc
    29101 aggggtccat gcacgagatc caaccgcctg ttcagctggt ccgtgacgat ctgctctatt
    29161 tcgtgcgtct gactggcggc ccggtcgctc aagtcgtact ggcgaatcac cggcgactgg
    29221 cctgcgtcag gaacctgaac gggaacggtg acgccagaca cgcagcggaa cgtggtgcga
    29281 aggatctcgt gtctctcgac gacgtgtctc accgtgtcgg cgaagatcgc ctcatcgaac
    29341 gctccgtcta ccaggacggt gcacgccgag cgacgattct gccagtcgcc gatctgctga
    29401 aggcgccaca accgatcctg ttgcggcgag agccggaaac cggcgacatc ttcctgcatg
    29461 cctgcggact cgtttcgagc gctcattcgc tcaccggcca ggtgtgtcga ttcgttcgcg
    29521 aggggaagca agaacgcatc gcgcgatcaa ccacggtggc gtgctgctct gccgcgtgcg
    29581 atcgcagaga gcttctctcg gctggcggac ttcgccagcg atttctgttt gccgcgatga
    29641 ctcgcgtcgg tgctgacgag aatgtcgccc accgtcctcg attcagcctc accggagctc
    29701 accagcgtcg tgatggccgc ttccagatag ctcaagatgt cggaaatttc tccgtcgtcg
    29761 aagcgacgtg cgtcgtagtt gacgcggcac aagacggccg gtcgcaaagt cacgtccagc
    29821 gtcagcgcgt agtgcgacgc gtcgtcggcg tgcaggtcgc ggatcacgag agccgattct
    29881 cccttcagcg tgccaaccgg gtggttcttg aacacgagga ggctttcgaa caggggcttg
    29941 ccacgcggca cgtcactcca gccgtgcacg tctaccagtg aggagtattc gtagtctcgt
    30001 tgcctgacct gacctgcctg tatcgccgtg agccatggca ccacgccgtc atcacggttc
    30061 agccgtacgc gcagcggcag tgtgttgatg aagagcccta cgatggaatc caccccctcg
    30121 agttggggag gacgcccgga cacggacacg ccgaacacga cgtcgtcgct gccggagatg
    30181 cacgccagga ccagccccca tgctccctgg agaacggtgt tcagcgtcac gtggtgccgc
    30241 cgggcgtagt cgcgcagcgc gtcggtggtc gacgacggca gcggcaacgg ccggcgtcga
    30301 taggaacgcc cggtcgcggt cgattcagcc gcggtcagcg acccgcccac gggagtcgga
    30361 acggtgaagc ccgccatcac gccgcgccag taggactcgg ctgccgtctg actctgcccg
    30421 gcgtgccagg cgatgtagtc gcggaacggg cgcgccggcg gcagctcgat cgcctcactc
    30481 cggcggaatc cctcgtacag gctcatgacc tcctggatga tcaaggggcc ggaccaccca
    30541 tccagcagca tgtggctgta gctccagacg aactggaacg ttcgctcccc gagcctcgcg
    30601 agcgtgacgc gcatgagagg ggcctcggtc agggcgaggc cccgatcgcg atcggctgcc
    30661 agaagagatg tcagttgcct ctgctggtca gtctgcgcaa gcgatcgcca atcctccacg
    30721 acccaaggca gcgcgacctg tccgtggacg atctggagtg gcctgtcgag attctcgtag
    30781 cggaaggtcg ttcgcaatgc gggatgccga tcgacgacct gttgccaggc ccgctgaaat
    30841 gcctcgaggt ccagatcccc ctcgagcgtg caactgagct gctcgaagta gcctcccgaa
    30901 tcacgatcgt agagcgagtg gaacagcaga ccctgctgaa gcggcgacag cggatagatg
    30961 tcctcgattc gcgagtcatc tcccaggagc gtatcgagcg tgcgctggtc caggtcggcc
    31021 agcggaaagt cggcgggcgt cagcttgcct gccgcggccg agggacgctg acgaatcagc
    31081 acacgcagcg attcgaggag gccggtcacg agggcctgcg cggtctcgcg ccgatgtttg
    31141 ccgccgaagg tgagtgagac ggtcagctga tccgccgcaa cgcccgccgc cagctccatc
    31201 aggtgcgggc gcgcgtttgc gggggcgtgc gccgggccgc gagactcggc cgcctgcgtg
    31261 aacaacgatg agccgggttc ggcttgatcg gccgtcccga gataaagaaa cacgatctcg
    31321 gcgtgcggca ggcgttccag ccgcgggccc gactccgggc tgagatagcg gagcaggccg
    31381 aaattcattc cgttattcgg aacggctcgg agctgctctt tcaccgtcct gatcgcgtca
    31441 ccgggtcgag cgcccggcct gatttgcagc gccacgggat agatcgtcgt gaaccacccg
    31501 gccgtacgag acacgtcgat cccctcgatg aactcctcgc ggccgtggcc ctcgagctcc
    31561 aggagcaatg ccgactggcc actccactta cagaaggcct gcgtggtggc tgtcagaagc
    31621 agttcatcga cgcgcgcgtt ccacgctttc cgtgcgtcgt cgagcagcag ctgcgtctcc
    31681 gacgcggtca gggacgacga gacagtggcc ttcgacccgt aggtgttctc gccatccggg
    31741 acgtctcgtg gcaacggttc cacgcccgcc tctcccaccg acagccagta cgggagctcg
    31801 cgagccacgg tctccgaccg ggcatgttcg ttgaggcgcg ccgaccagtc cttgaacgac
    31861 accgtctttg gcggcagcgt gacgttttcg ccccgaccga gcgcttgata gatcgcctcg
    31921 aagtcctcca gcaggaaccg ccaggacagg ttgtccacgg ccaggtggtg aatgcacacg
    31981 agccagcgcc cgcgccgccc ggcacccagg tcgaaatgtg tggcctgcat gagagggccg
    32041 cgcgacagat ccagcgcgcc ctgcgactgc gccgcggccg cctcgatggc ggccgccacc
    32101 tcgccatccg gcacggtact cagatcgacc tgacgcacga cggcttcggc gcccggcttg
    32161 gcgacgatct gccgccaacc cgactcgtcc cgcgtgaagc gcgcgcgaag ggcgtcgtga
    32221 tgcgcctcga ggcgcgtgag cacgtcgcgt acgcgggcca cgtcgagcgg ctggacttct
    32281 tccagaagaa cggattgatt gaagtgatgc ggcgccgcga actgacgctc gaagaaccag
    32341 tgctggatcg gcgtcaacag cacggggccc gtgaccacgc cctgctcggc ctcgatcctc
    32401 gaggcgccgg aggcgaccga tgccagctcc gcaatggtct ggtgctgaag gatgtgcttg
    32461 atcgagagcg ccaggccagc ctggttggcc ttggcgacga cctgaatgcc gcccaccgaa
    32521 tcgccaccaa gctcgtagta gttgtcgtgg atgccgatcg gctcgatgcc cagcacgtcc
    32581 tgccagatcc gggtcaacga ccgttccgaa tcgctgcgcg gcgcctcgta ttcggtctgc
    32641 agttccggcc gcgggtgtgc cgagggcctg gaccctcggg cgtcgggcgc tgccgcctgc
    32701 accggcgcgg cagtcttgcg ctcgtcggac gccgcagcct ccacgccgcg aatcgccacg
    32761 aggacctgtg ccgcgtcgct cgccaggatg cgcgagaaga tgtcgacacc ctggctgttc
    32821 ttcaacccga actcggggaa cacctctcgc gtcgaaccgt cgtcgtcttt cctcgactgc
    32881 acggccatgc cgacgtcctg ccaggtgtcc cagttgatcg acgtgacgaa gccgtcacgc
    32941 gccttggccc ggtggcgagc gtacacatcc agaaatcggt tggcggccga gtactccgag
    33001 cggccgggca gccccaggaa cgccgtctgc gaggagaaga ggacgaagaa gtccaggtgc
    33061 tgggtgcgca gcaactcgtc gagcacctgc gtgcccgaga cctttgcggc aaacacacgc
    33121 cgcactgttt ccggtgtctg ccggagcatc actccaccgc ccggcagccc ggcggcgtga
    33181 acgacgccgt tcacgggtcc gaagcgagcg ttcgccaatg cgaggacgtc ggacatctgc
    33241 gccttgtcgg agacgtccgc ctgacatacc agcacttccg ccccgtttgc ctccatggac
    33301 cggatactgc gtatgcgccg gcccacgggg tcggccggac cggcgctgcc ggcgagctca
    33361 tcccacttct tcctgtcagg cagctccgtg cgtcccacca gcacgagtct tgcgcgcacc
    33421 gttcgagcga gatgctccgc cagagcgaga ccaacgccgc cgagaccacc ggtgatcaga
    33481 taggtgccgc gctcgcgcag ccgcgcaggc ggctcggccc ctgccgcaag cggcatcggc
    33541 tcgaaggact ggagccatcg gtagtttcca cgccacgcca ccacgaggtc ggtcgcgttc
    33601 gaggtcgcct ccgccagtac gtttcgcgcc tgcgttcgaa cgtcgtcggg gcccgcgagc
    33661 gagatgtcca cgcagcgccg tcgaatgtgc ggaaactccg tgccgatcgt catcgccgcg
    33721 ctgacgaccg tggccttgtc cgggcagagc tgctcgtcgc ccgtgacgtc ctgcacctgg
    33781 ctcgtcacta ccgagagctg catgggcgag gtcgcctccg cctcgctcaa tgcctgcacg
    33841 agatacagaa ggctgtagaa gcctcgctgc tgccgctgct cgaacagctc ggtgctcgtg
    33901 tggtacggcg cgtcccggtc tacggaccac atgtggacca tgtggctggg aacgccagcg
    33961 cccttcgaga gctcggtgaa gagacggacg tagtccgccc gccggcccgg atcgatcgcg
    34021 aacgtccgct cggacctgcg ctcgaagccg cttccgatcg acacacagat cgcgtcggag
    34081 ccttgcgagg ccagcatctg ctccatctgc cggccgacgc cgctgtcgtc cacgaacagg
    34141 agccatgtct ctttctcgag cgggggacca ggtcgcaggg tggatcgctt ccacgacggt
    34201 tcgaacagcc agtctgtgac gtcgcgcctc ggtgcaggct tctcctccac tgtgctgctg
    34261 ccaggagcaa tccagtaccg ctgtcgctcg aacggatacg tcggcagcgg cacccggcgc
    34321 cgtttctcgc ctggatagag tgccggccag tccggccggc agccgcgcat ccacagctgg
    34381 ccgaggctct gcagcatgtg ctcgaggtcg gactccttgt ccttgacgtg gggaagcgac
    34441 gccaggatgg tcgttcgttc gtgcttcgcc tggtgcgccg ccgccaggga acgcagagtc
    34501 gttccaggcc cgacttcgag cagaatcctg ttcggctcgg caagcagctg ctcgacaccc
    34561 tgtgaaaacc gaacggcctg tcgcaggtgg cgcgcccaat acgacgggtc tgttgcgtcg
    34621 ctcgcttgta tccaatcacc cgtgagattg gaaatgaagg gaatcgcggg cggctgcaag
    34681 tgcacgcggc gcatcagatc ggcgaacggc tcgacgatcg ggtccatcat cgccgagtga
    34741 aacgcatggg acgtgcgcag ccgctgggtc gcgcagccct gcttctcgag cgtggcttcc
    34801 agtacatcaa ccgcggcctt cggtcccgac gcgacacaaa gtcgagggcc gttgatcgcc
    34861 gcgagcgaca gtccatcacc gaggagaggc tcgagctcgg actcggacag cgggatcgac
    34921 agcatgtcgc cggtcggcat ctcgcccatc agccggccgc ggcgggcgac gatggccacg
    34981 gcatcctcca gcgtgaacac gccagccagg cacgcggccg tgtactcgcc aatgctgtgc
    35041 ccgatcatcg cctgcggctt gatcccccaa ctgatccaga gcctggccag ggcgtactcg
    35101 aacgcgaaca tcgccggctg cgtgaactgc gttcgatcga gctgcctggc cgcctcctcc
    35161 gtccgctccg cagccgggta gagaaggtct cgcaagtcga gcccaaggtg cgcccggaac
    35221 gcgtcgcagc actcgtcgag gtgccggcgg aacgtggctt cggtgcgata gagctcagcg
    35281 cccatattgg cgcgctgcgc gccctgaccg gtgaacatga agacgaccac cgggtccgtg
    35341 ccgtcgtggg tgtgactgct gaccggcttg tggttcggct tctcgagcag tgcaatcgcg
    35401 tcctctcggt tctcgcagac gaacatccga cggtgaggga gcacgctgcg cccgacctgc
    35461 agcgtatacg cgacgtcggc gagggccgtc tcggaacccg ctcgcaggtg gtcggcaagg
    35521 ttgctcgttg ctcgagccag cgccgccgcg ctcttcgccg acaacaccac gagctggcgc
    35581 gcgcgggaac cgggggccga ctcgagctgc ggcgcttctt ccagcacaag gtgggcgttg
    35641 gtgccgccga tgccgaacga actgaccccc gcccgtcggg gtgtcggccc ctcgggccat
    35701 tcggcgagct tgtcgttgac gaaaaacgga gtggagtcga aatcgatctc cgtattcggc
    35761 cgctcgaagt gcaggctcgc cgggatgcga cgattctcga gtgccagtac cgtcttgatc
    35821 agacccgcga cgccagcggc gacgtcgagg tgcccgatgt tggttttcac cgacccgatc
    35881 gcacagaacc gcttcctgtc cgtcccggcg tgaaacgcct gtgtcaatgc gctgatctca
    35941 cacggatcgc cc
//
LOCUS       DBNGSH010000429         3902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_718705, whole genome shotgun sequence.
ACCESSION   DBNGSH010000429 DBNGSH010000000
VERSION     DBNGSH010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_718705"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="AC16CY_09770"
     CDS             <1..969
                     /locus_tag="AC16CY_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3354118.1"
                     /translation="WTGVPVGRMVKNEIEAVLNLAETLNQRVIGQRHALEMIAKRIQT
                     SRARLDNPNKPIGVFMLCGPSGVGKTETALTLAEALYGGEQNVITINMSEFQEAHTVS
                     TLKGSPPGYVGYGEGGVLTEAVRRRPYSVVLLDEVEKAHSDVHELFFQVFDKGWMEDG
                     EGRRIDFRNTIILLTSNVGTDLIMGMCKDPELMPEPEGIAKGLREPLLKKFPAALLGR
                     LVVIPYYPLGDAMLANIVRLQLGRIARRVTEHHKIPFHYDDEAVKLIVSRCTEVESGG
                     RMIDAILTNTVLPAISREFLARTMAGGGLNGVRLAVADGDFAYRFD"
     gene            1033..2367
                     /locus_tag="AC16CY_09775"
     CDS             1033..2367
                     /locus_tag="AC16CY_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3354119.1"
                     /translation="MEQSPTKDRAVTLEEAVSLAIGLQKKEQWSAARDLYKRVLEVAP
                     DHADALHFSGVLAHQEGHSDEAVALIRRSLELQPHQADWHSNLGVVLRDRLELDEAVA
                     ACRRAIAIDPDHANAHNNLGVLLRAQGDLAGAEDAYRAAIRLSPEHSDAYTNLGVLLN
                     AQKRTHEAVICFCKVITFRPKHPEARRLLALAHCTLGDAEAAVRVFDEWLAEEPGHPI
                     AVHMRAACSGKDVPARASDGFVEATFDGFAASFDSKLAKLLYRAPELVSGMVADSSAV
                     ASKQFDVLDAGCGTGLCGPLLSPYARRLVGVDLSAGMLTQARARKIYDELVKAELTAY
                     LREQTGAFDVIVSADTLVYFGPLQDVAAAAAQALRPGGRLIFTVEALADGESSAGYLL
                     SNHGRYCHTRRYLECVLTGARLQPEIVPAELRLEAGDPVEGFVVRATKAARN"
     gene            2371..>3902
                     /locus_tag="AC16CY_09780"
     CDS             2371..>3902
                     /locus_tag="AC16CY_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HIR3354120.1"
                     /translation="MPRVMEITTPLGDDVLLFHGMHAREEMGRLSELQLDLLSPKNDI
                     NLDEILGKSVTVKLALPDDSTRYFNGIVSRFAQGGKYGRYHRYFAAVRPWLWLLTRTS
                     DCRIFQDMAVPDIIKAVFGDEKTADFKFELTGKYRKWTYCVQYRETDFNFVSRLMEEE
                     GISYYIKHTDGHNTVVLTDSTSKHTPFPGYDKLPFIAPDQLVRPELEHVSSWDFSREI
                     QPGVYVHDDYDLERPSVKLKTSKVLSRGYSPSDYEVYDYPGHYLQKADGEQYAGVRID
                     EYGSQFEIAQAATNARGVCVGSLFTLEDCPRDDQNCEHLIVAANHDLEFSDYEAMPEG
                     SGTSYRCSFVAMSSKQQFRPRRRTPKPFVQGPQTAVVVGPAGDQIYTDKYGRVKVQFH
                     WDRYGKKDENSSCWIRVSHPWAGKGWGAVSTPRIGQEVIVDFLEGDPDQPIITGRVYN
                     AENQPPFGFPAGAVISGMKSDTHKGGGYNEMSFDDTAGKEKITVHGQYDMNSVIEHDQ
                     TLT"
BASE COUNT          727 a         1244 c         1331 g          600 t
ORIGIN      
        1 tggacgggcg tgccggtcgg ccgcatggtg aagaacgaga tcgaggccgt gctgaacctg
       61 gccgagacgc tcaaccagcg cgtgatcggc cagcgtcatg cgctcgagat gatcgccaag
      121 cgcatccaga cgtcgcgagc ccggctcgac aacccgaaca agccgatcgg cgtcttcatg
      181 ctctgcggtc cctccggcgt cggcaagacc gagacggcgt tgaccctggc cgaggcgctc
      241 tacggcggcg agcagaacgt catcacgatc aacatgagcg agttccagga ggcgcacacc
      301 gtctcgacgc tcaaggggtc tcctccgggc tacgtcgggt acggcgaggg cggcgtgctg
      361 accgaagcgg tgcggcgccg cccgtacagc gtcgtgctgc tcgacgaggt cgagaaggcg
      421 cactcggacg tgcacgagct gttcttccag gtcttcgaca agggctggat ggaggacggc
      481 gaggggcgcc gcatcgattt caggaacacg atcatcctgc tgacctcgaa cgtcggcacg
      541 gacctgatca tgggcatgtg caaggatccc gagttgatgc cggagccaga ggggattgcg
      601 aagggcctgc gcgagccgct cctgaagaaa ttcccggctg cgctgctggg gcggcttgtg
      661 gtgatcccct attacccgct cggcgatgcg atgctggcca atatcgtccg gctgcagctc
      721 ggccggattg ccaggcgggt gaccgagcac cacaagatcc cgtttcacta cgacgacgag
      781 gcggtcaagc tcatcgtcag ccggtgtacc gaggtcgaga gcggcggacg tatgatcgac
      841 gccatcctca cgaacaccgt gctccccgca atcagccgcg agtttcttgc tcgcacgatg
      901 gcgggaggcg ggctgaacgg cgtacggctg gcggtggccg acggcgattt cgcgtatcga
      961 ttcgactagg ggtcagatct tgatttttgc acgccgcgca aaaatcaaga tctgacccca
     1021 tgctgaggca gcatggagca atcgccgacc aaggatcgcg ccgtcacgct ggaggaggcg
     1081 gtgtcgctcg cgatcgggct gcagaagaaa gagcagtggt cggccgcgcg cgacctctac
     1141 aagagagtgc tggaggtcgc cccggaccac gccgacgccc tgcacttttc gggagtgctg
     1201 gcccatcagg aagggcacag cgacgaagcc gttgcattga tcaggcggag cctggagctt
     1261 cagccgcatc aggcggactg gcacagcaac ctgggcgtcg tgctgcgcga tcgcctcgag
     1321 ctggacgagg ccgtcgccgc gtgccgccgc gcaatcgcga tcgacccgga tcacgccaac
     1381 gcgcacaaca atctcggcgt cctgctcagg gcacagggcg atctcgccgg tgccgaggac
     1441 gcctatcggg ctgcgattcg cctcagcccc gagcactccg atgcctacac gaacctgggc
     1501 gtcctgctga acgcccagaa gcggactcac gaagcggtca tctgcttctg caaggtcatc
     1561 accttccggc cgaagcaccc cgaagcccgc aggctgctcg ccctggcgca ctgcaccctg
     1621 ggtgatgccg aagccgcggt gcgcgtattc gacgagtggc tcgcggaaga gcccggccat
     1681 ccgatcgcgg tgcacatgag ggccgcgtgc tcggggaagg acgtgccggc ccgcgcctcc
     1741 gacggcttcg tcgaggcgac cttcgacggc tttgccgcca gcttcgattc caagctcgcc
     1801 aagctcctct atcgcgcgcc cgagctcgtg tcgggcatgg tggcggactc cagcgccgtg
     1861 gcgtcgaaac agttcgacgt gctcgacgcc ggctgcggca ccggattgtg cggaccgctg
     1921 ctctcgccgt acgcgcgccg gctggtgggc gtggatctgt cggcgggaat gctcacccag
     1981 gcccgtgcgc gcaagatcta cgacgagctc gtcaaagctg aattgactgc gtacctgcgc
     2041 gaacagacgg gggccttcga cgtgatcgtg tcggcggaca cgctggtcta cttcgggcca
     2101 ctccaggacg tggcggcggc ggcggcgcag gcgctgcgtc cgggcggccg gctcatattc
     2161 accgtggagg cgctggccga cggcgagtcg tctgcggggt acctcctcag caaccacggg
     2221 cggtattgcc acacgcgccg gtacctcgaa tgcgtgctga cgggtgcgcg gcttcagccg
     2281 gagatcgtgc cggcggagct gcgcctcgaa gccggcgatc cggtcgaggg attcgtcgtg
     2341 cgggcgacga aagcggcgag gaattagacc atgccgagag tcatggaaat cacgacgccg
     2401 ctcggggacg acgtgctgct gttccacggc atgcacgcgc gcgaggagat gggacggttg
     2461 agcgagctcc agctcgacct gctcagcccg aagaacgaca tcaacctcga cgagatcctc
     2521 ggaaagagcg tcacggtgaa gctggcgctg cccgacgaca gcacgcgcta cttcaacggc
     2581 atcgtgtcgc ggttcgcgca gggcggcaag tacggccgct atcaccgcta cttcgccgcg
     2641 gtgcgcccgt ggctctggct cctgacccgg acgagcgatt gccgcatctt ccaggacatg
     2701 gcggtgcccg acatcatcaa ggcggtgttc ggcgacgaga agacggccga tttcaagttc
     2761 gagctgaccg gcaagtaccg gaagtggacc tactgcgtgc agtaccggga gaccgacttc
     2821 aacttcgtca gccggctgat ggaggaggag ggcatctcgt actacatcaa gcacaccgac
     2881 ggccacaaca cggtggtgct caccgactcg acgagcaagc acacgccgtt tccgggctac
     2941 gacaagctcc ccttcatcgc cccggatcag ctcgtgaggc cggagctcga gcacgtgagc
     3001 agctgggact tctcgcgcga gatccagccg ggcgtctacg tccacgacga ctacgacctc
     3061 gagcggccca gcgtcaagct gaagacgagc aaggttctgt cgcgcggcta ctcgccgagc
     3121 gactacgagg tctacgacta ccccgggcac tacctgcaga aggcggatgg ggagcagtac
     3181 gccggcgtgc ggatcgacga atacggcagc cagttcgaga tcgcgcaggc cgccaccaac
     3241 gccagggggg tgtgcgtcgg ctcgctgttc acgctcgagg actgcccgcg cgacgatcag
     3301 aactgcgagc acctcatcgt ggcggcgaac cacgacctgg aattcagcga ctacgaagcg
     3361 atgccggagg gctcgggcac gagctaccgg tgcagcttcg tggcgatgtc gagcaagcag
     3421 cagttccggc cgaggcgtcg cacgccgaag ccgttcgtgc agggtccgca gacggcggtg
     3481 gtcgtgggac cggccggtga ccagatctac acggacaagt acgggcgggt gaaggtgcag
     3541 ttccactggg accggtacgg caagaaggac gagaacagct cgtgctggat ccgcgtgtcg
     3601 cacccgtggg cggggaaggg atggggggcg gtgtcgacgc cgcgcatcgg ccaggaagtg
     3661 atcgtcgact tcctcgaggg cgatcccgat cagccgatca tcaccgggcg cgtgtacaac
     3721 gccgagaacc agcccccgtt cggcttcccg gcgggcgccg tcatcagcgg catgaagtcg
     3781 gacacccaca agggcggcgg ctacaacgag atgtcgttcg acgacaccgc gggcaaggag
     3841 aagatcacgg tccacggcca gtacgacatg aacagcgtga tcgagcacga ccagacgctg
     3901 ac
//
LOCUS       DBNGSH010000430         5344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3604912, whole genome shotgun sequence.
ACCESSION   DBNGSH010000430 DBNGSH010000000
VERSION     DBNGSH010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3604912"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="AC16CY_09785"
     CDS             complement(<1..514)
                     /locus_tag="AC16CY_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3354121.1"
                     /translation="MPAPRFKTAIYEQIARIGKAASSPGRLELLELLTQGPKTVEALA
                     RQTNQSLANASHHLKVLRTSRLVEAEKAGLYVTYRLADANVGEFLLHLRTLATTRLAE
                     IELVTRQYLEHRGALEAVRDEELLRRVRAGEVTLLDVRPREEYLAGHIPGAMSVPLAD
                     LKARARRLPKS"
     gene            complement(595..972)
                     /locus_tag="AC16CY_09790"
     CDS             complement(595..972)
                     /locus_tag="AC16CY_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354122.1"
                     /translation="MTSSGRPKPQGPVSAQVFLRSVSGRSIHELGSATDVGDLAPFRA
                     PAAARAAAVREFERLGFSVYEDEMGLAPSIQAPPSVFRLVFGVPEAEGRAPSARESIR
                     LRVPDDLRDIVEDILLLPPPEFH"
     gene            complement(1148..2632)
                     /locus_tag="AC16CY_09795"
     CDS             complement(1148..2632)
                     /locus_tag="AC16CY_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3354123.1"
                     /translation="MAKKSRRPSRPSRAPAQTVQKVDRELSLPRTETVSFEVVCRPRS
                     GAGLPQLMGELSMDTLNDSTPDDRTLFDVSKRLQEQGFTVFVDDASSSVSAEGPADLF
                     EKTFKTRLRKRGRTLKAATREHSFEFIDTAKDAPEPDPVNVPGSLFVSIQRPPIFFAS
                     PLPPPIRYFHLRVPGDVAMLMHASATHRRTLPLGTAGATGHGVKVAMLDTGFNVQPYF
                     ARHGYRLNPIAAADAPPPANLDANGHGTGEVANIFACAPDAQVFGVKMGGNAVLSFDR
                     AMSVNPRVISCSWGFHLPGVTTLPPALVPLRLRIMSVVAAGVTVVFSAGNGHVGFPGM
                     MPEVISVGGVFADQNVNLRASDYASSFTSLIFPGRRVPDFCGLVGMRPKATYIMLPIP
                     PSCEIDADLGGVPQPNKDETATNDGWGVFSGTSAAAPQVAGICALLLQKRPTLTPAQV
                     KAILRGTATDVTVGVSAMGQPAGPGVDAATGSGLVHALNAWLSA"
     gene            3284..4618
                     /locus_tag="AC16CY_09800"
     CDS             3284..4618
                     /locus_tag="AC16CY_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3354124.1"
                     /translation="MARRLLVLGMLCFSFHADRAALAQAPDHLRSTDARRVDAGPPLT
                     LAGAIEEALARNPTLLVLRRQFEAARHRPAQARALAAPTFEAQIWQWPVDTINPLNTN
                     MYMFTVQQELGRGSKRDLAAAVAEKDVELTSIEIALRARDVINEVTRAYTDVSLARRA
                     IDIHLESVALLRQFADLSSVKYAAGRTTQQDVLKAVVEVSMLHDDLVMHEESEANAAA
                     RLNTLLDRDPHAGIGPLAEPREAIALPSSDELQRQAMEHQPELAAAKLQVERAKAALA
                     AVDGDYKPDFFVGGGYMLTPRQAGAWTASVGMSWPNAPWSRGRLVARKAEAAADVETA
                     AMRVKAAERSIQLAVHDAYVRTVAAGRRALLLESTIVPQSVQTLEVSRVAYQTDRSDL
                     RALIDNQRTLLEARLSFYRALGERELAFADLERAVGAPIHSAAAHVPATEVR"
     gene            4621..>5344
                     /locus_tag="AC16CY_09805"
     CDS             4621..>5344
                     /locus_tag="AC16CY_09805"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3354125.1"
                     /translation="MAMTTRRSVGIALGVVIAAALLVVAFAFRDRLRPQPEVSGAADR
                     PPEPPAQAGAGGDIVARGDVTIDPQRQQLIGVRLAAATRSRMATVVRTTGVVRIDETR
                     LSDVNVKLDGWIRDLHVDFTGQAVQKGQPLFTLYSPDLLTTENEYLLALKTRDQVKDS
                     AIADAREYADRLVDAGRQRLRLWDLPAEDIARLEETREAPEIMTFRSPVSGVVVEKQA
                     VRGMHIVPGQTLYKVADLSVVWV"
BASE COUNT          877 a         1754 c         1851 g          862 t
ORIGIN      
        1 ggctcttcgg caggcggcgc gcgcgcgctt tgaggtccgc cagtggcacc gacatcgccc
       61 ccgggatgtg gccggcgaga tactcctccc gcggacgcac gtcgaggagc gtgacctcgc
      121 cggcgcgcac gcgccggagg agttcctcat cgcggacggc ctcgagcgcg ccgcgatgtt
      181 cgaggtactg ccgcgtgacg agctcgatct cggcgagacg ggtcgtagcc agagtccgca
      241 agtgaaggag gaactcgcca acattggcgt cggcgagccg gtaggtgacg tagagcccgg
      301 ccttctcggc ctcgacgagc ctggaggttc gcagcacctt caagtggtgc gacgcgttgg
      361 cgagggactg attggtctgg cgggcgagcg cctcgacggt cttgggcccc tgggtgagca
      421 gctcgaggag ctcgagccgt cccgggctcg aggcagcctt gccgatgcgg gcgatctgct
      481 cgtagatggc ggtcttgaag cggggagcgg gcatattcaa atactcaaac agatatttga
      541 atagtgctcc cgggggcggg ctgaagtcaa gccggcggcg atcgcccgcc ggaatcagtg
      601 aaattccggc gggggaagca gcaggatgtc ttcgacgata tccctgaggt cgtcgggtac
      661 gcggagccgg atcgactctc gcgccgaggg cgcgcgcccc tcggcctcag gcaccccgaa
      721 aaccagtcga aacaccgatg gcggcgcctg tatcgacggc gccagcccca tctcgtcctc
      781 gtagaccgag aacccgagac gctcgaattc ccgcaccgcc gcggcgcgcg cggcggccgg
      841 ggcgcgaaac ggggcgagat cgccgacgtc ggtggcgctg cccagttcgt ggatcgaccg
      901 gccggagacc gagcgcagga acacctgggc ggagacgggg ccctgcggct tcggtcgtcc
      961 ggaggaagtc attcgtgtct catcatacgc ggcggcacga tggggcattg cagcaagtcg
     1021 ccccgtgaac aacgaacccc gaaccccgaa cacagaacac gaacctgaaa aactgaacac
     1081 gaaccgagaa ccgggaagag agaagcgtga acggcaagat ctctgttcaa ttcatgtccc
     1141 tccgggttca ggcgctgagc caggcgttga gcgcgtgcac caggcccgag ccggtggcgg
     1201 catcgacgcc gggccccgcg ggttgtccca tcgcgctcac gccgaccgtc acgtcggtgg
     1261 ccgtgcccct cagaatggct ttgacctgcg ccggcgtcaa cgtcggccgc ttctgcagca
     1321 gcagcgcgca gatgccggcc acctgcggag cggccgccga cgttccactg aagacgcccc
     1381 agccgtcgtt ggtggctgtc tcgtccttgt tgggctgcgg cacgccgcca agatcggcgt
     1441 cgatctcgca cgacggcggt atcgggagca tgatgtaggt cgccttcggc cgcatcccga
     1501 ccagtccgca gaagtccggc acgcgccgtc cggggaagat caggctcgtg aagctggagg
     1561 cgtagtcgct cgcgcgcaga ttcacgttct ggtccgcgaa cacgccgccc accgagatca
     1621 cttcgggcat catccccggg aatcccacgt gcccgttgcc cgccgagaag acgacggtga
     1681 cgccggcggc caccacgctc atgatccgca ggcggagcgg caccagcgcc ggaggcagcg
     1741 tcgtcacgcc cggcagatgg aagccccagc tgcacgagat gacgcgcggg ttgactgaca
     1801 tcgccctgtc gaagctgagg acggcgttgc ctcccatctt cacgccgaac acctgcgcgt
     1861 cgggggcgca cgcgaagatg ttggccactt cgcccgtgcc gtggccgttc gcgtccaggt
     1921 tcgccggggg cggcgcgtcg gccgccgcga tcgggttgag ccggtagccg tgcctcgcga
     1981 agtacggctg cacgttgaat cccgtgtcga gcatggcgac tttgacgcca tggcccgtcg
     2041 caccggcagt tccgaggggg agcgtgcggc ggtgcgtcgc cgaggcgtgc atcagcatgg
     2101 cgacgtctcc ggggacccgc aggtggaagt agcggatcgg aggcggcagc ggcgaggcga
     2161 agaagatcgg aggccgctga atcgacacga agagagatcc aggaacgttg accgggtccg
     2221 gctccggcgc gtccttggcc gtgtcgatga actcgaacga gtgttcgcgc gtggcggcct
     2281 tcagcgtgcg gccccgcttg cggagcctgg tcttgaacgt cttttcgaac aggtctgcag
     2341 gtccttccgc ggacacgctc gagctggcgt cgtcaacgaa cacggtgaag ccctgttcct
     2401 gcagccgctt cgagacgtcg aacagcgtgc ggtcgtcggg cgtcgagtcg ttcagcgtgt
     2461 ccatgctgag ctcgcccatc aactgcggca ggcccgcccc ggacctgggg cggcagacca
     2521 cttcgaagga aacggtttcc gtgcgcggga gagacagctc gcggtccacc ttctggacgg
     2581 tctgggcggg cgcgcgcgac ggtcgcgacg ggcgacggga tttctttgcc atagggtcct
     2641 ctttcgtacg tagtgtccgg cttcagccgg accgttcggc gagaatgcag gttcgtgcat
     2701 tcatcaacga cggcggacgg caatgcgaac agggccgggt ttgacttccg tctttctagg
     2761 cgagaagatg agttgacgat ggttcgaggg atgacgacca gccgactcat ggcggcagcg
     2821 ctgtcgctgg cactctttgc gtcttccgtc tcggttgcct acccgtccga agtgagggaa
     2881 gcgcggaagg cctgctgcgc ggcgatgcac agcgactgtg gggctgcagt gacagcgcga
     2941 aagtgctgcg cgccggatga tgcaggttcg gccgcgctga taccagacac ctctcagtca
     3001 cgccagcgtg cggtcgtggc tgttggcccc ccgctcgatc tagccttctc gactctcgcc
     3061 ggcactgtcc ggaccgcacg tacgagcccg ccgcgatcat cgcgcacgcc cacgtacctc
     3121 tccgactcgg tcgtccgcat ctagtctttt cgctgtttgt tcgtgcggcg ccgcgcggca
     3181 tttgcccgcg tgcgtgtcgc gtgacgcatc ccttgtttct tctgcagtgt ccggcttccc
     3241 tggcctgagc gaggtcgaag gcaaccggac gatggattgc gatatggcgc gaagactgct
     3301 cgttctcgga atgctctgct tctcgttcca cgcggaccgt gccgcccttg ctcaggcgcc
     3361 ggatcacctg cgcagcaccg acgcgcggcg cgtcgacgcc gggccgcctc tgacactggc
     3421 gggagccatt gaagaggcgc tggcgcggaa cccgacgctc ctcgtcctcc gtcgccagtt
     3481 cgaggccgcg cggcacaggc ccgctcaggc ccgcgccctg gcggctccca ccttcgaagc
     3541 gcagatctgg cagtggccgg tcgatacgat caatccgctg aacacgaaca tgtacatgtt
     3601 cacggtgcag caggaactcg gccggggcag caagcgcgac ctcgctgcag ccgtggccga
     3661 gaaggacgtg gagctcacct cgatcgaaat cgcgctcagg gcccgggacg tgatcaatga
     3721 agtgacgcgc gcgtataccg acgtgtcact cgcccggcgc gcgatcgaca tccatctcga
     3781 gagcgtggcg ctgctgcggc agttcgccga cctctcgagt gtcaagtacg ccgccggccg
     3841 cacgacacag caggacgtcc tcaaggccgt cgtcgaagtg tcgatgcttc acgacgatct
     3901 cgtgatgcac gaggagagcg aggccaacgc cgcggcgcgg ctgaacacgc tgctcgatcg
     3961 cgacccgcac gccgggatcg gcccgctcgc cgaaccgcgc gaagcgatcg cactgccctc
     4021 gtccgacgag ttgcagcgcc aggcgatgga gcatcagccg gagcttgctg ccgcgaagct
     4081 ccaggtggaa cgggcgaagg cggcgctcgc ggccgtggat ggcgactaca agcccgactt
     4141 cttcgtcggc ggcggttaca tgttgacgcc gcgccaggcg ggcgcctgga cggcgtcggt
     4201 cgggatgtcg tggccgaacg cgccatggtc cagaggccgt cttgtcgcca gaaaggccga
     4261 ggccgcggcc gacgtcgaga cggccgcgat gcgggtcaag gctgcggagc ggtcgattca
     4321 actcgccgtg cacgacgcct acgtccgcac ggtcgccgcc gggcgccgcg cgctcctgct
     4381 cgagagcacc atcgtgcccc agtcggttca gacgctcgaa gtctcgcgag tcgcgtatca
     4441 gaccgaccgc tcggacctcc gcgcgctgat cgacaaccag cggacgctgc tcgaggcgcg
     4501 cctgagcttc taccgcgcgc tgggggagcg cgagctcgcg tttgccgatc tcgagcgcgc
     4561 agtcggcgca ccgattcatt cggccgcggc tcacgtgccc gccaccgagg tgaggtagtc
     4621 atggcgatga caactcggcg gtcggtcggg atcgcgttgg gcgtggtgat cgcggcggcg
     4681 ctgctcgtcg tcgcgtttgc cttccgggat cgcctccgcc ctcaacccga agtcagcggc
     4741 gccgcggatc gaccgccgga accgccggcg caggccggtg cgggcggcga catcgtcgcc
     4801 cgcggggacg tcacgatcga tccacagcgc cagcagctca ttggcgtccg cctggccgcg
     4861 gccacgcgct cacgaatggc aacagtggtg cgcacgaccg gcgtcgtccg gatcgacgag
     4921 acgcgcctgt cggacgtcaa cgtgaagctc gatgggtgga ttcgagatct ccacgtcgac
     4981 ttcacgggac aagctgtcca gaaaggacaa ccgctgttta ccttgtacag tcccgacctg
     5041 ctgacgacgg agaatgagta cctgctcgcg ctgaagacgc gagaccaggt gaaggattcg
     5101 gcgattgccg atgcgcgcga gtacgccgac cgcctcgttg acgccgggcg ccagcggctc
     5161 aggctgtggg atctgccggc ggaggacatc gcgcggctgg aggagacgcg cgaggctccg
     5221 gagatcatga cgttcaggtc tccggtcagc ggagtcgtcg tcgagaagca ggccgtgcgc
     5281 ggcatgcaca tcgtgcccgg gcagacgctg tacaaagttg ccgatctctc ggtcgtgtgg
     5341 gtcg
//
LOCUS       DBNGSH010000431         1579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_378937, whole genome shotgun sequence.
ACCESSION   DBNGSH010000431 DBNGSH010000000
VERSION     DBNGSH010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_378937"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="AC16CY_09810"
     CDS             complement(<1..820)
                     /locus_tag="AC16CY_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3354126.1"
                     /translation="MTTRVALVIAVVLVALGAAARAQVTSDRLLRAAQEPQNWVTYSG
                     DYASRRHSPLTEITPGNVRNLELKWILPNQVFGAWQSTPLVVDGIMYVTQRPNDVLAV
                     DAKTGRVFWQYRYTVSPDARVCCGANNRGVAILGDMLYLGTLDAHLVAIDRTSGRSLW
                     DVPVADPKLGYSITMAPLVVKDKVLVGSGGGEYGIRGVVAAFDAKTGKELWRFHTIPG
                     PGEKGNETWGGDSWKTGGGSIWVTPSYDPALNLTYWGVGNPGPDWNPDQRPGDNL"
     gene            complement(820..>1579)
                     /locus_tag="AC16CY_09815"
     CDS             complement(820..>1579)
                     /locus_tag="AC16CY_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3354127.1"
                     /translation="LLTIAVLCVLAAPADAQLLSLDHPGQYNQEDIARGSLLYTAQCA
                     HCHGRFGEEVSGVDLRRGLFRRSRSDEDVAQVITRGTPGGMPPFKLDAAELTGIVAFI
                     RAGFDTSASVKVGDAARGRTIFQGKGECTTCHRVEGRGPRSAPDLSDIGLARAAVVLE
                     RTVRDPTSSLLPINRPVRVVTADGRTIRGRRLNEDTHTVQIIDDRERLLSLAKKDLRS
                     FEVAKESAMPAYAGRLTSDEIADVVGYLLTLRKQ"
BASE COUNT          244 a          532 c          541 g          262 t
ORIGIN      
        1 acaggttgtc gcccgggcgc tgatcgggat tccagtccgg gccggggttg ccgacgcccc
       61 agtaggtgag gttgagcgcg ggatcgtacg agggcgtcac ccagatcgac ccgccgccgg
      121 tcttccacga atcgcctccc caggtctcgt tccccttttc gccggggcct ggaatcgtgt
      181 ggaagcgcca cagttccttt ccggtcttcg catcgaacgc ggcgacaacg ccgcggatgc
      241 cgtattcgcc gccgccggag ccgacgagga ccttgtcctt gacgacgagc ggagccatcg
      301 tgatcgagta gcccagcttt ggatcggcga cggggacgtc ccagagcgat cgtccgctcg
      361 tcctgtcgat cgccacgagg tgcgcgtcga gcgtgcccag gtacagcatg tcgccgagaa
      421 tggcgacgcc gcggttgttc gcgccgcagc acacgcgcgc gtccggcgag acggtgtagc
      481 ggtactgcca gaacacgcgg ccggtcttcg cgtccacggc caggacgtcg ttgggacgct
      541 gcgtgacgta catgatcccg tccaccacga gcggcgtgga ctgccacgcg ccgaacacct
      601 ggttgggcag gatccatttc agctcgaggt ttctgacgtt gccgggagtg atctcggtca
      661 gcgggctgtg ccggcggctc gcgtagtcgc cggaataggt gacccagttc tgcggctcct
      721 gcgccgcgcg cagcagccga tcggaggtga cctgcgctcg cgcggcggcg cccagcgcta
      781 cgagcacgac cgcgatgacg agcgcgacgc gtgttgtcac tactgcttcc tcagcgtgag
      841 cagatacccg acgacgtccg caatctcgtc gctggtcaac ctgccggcat acgccggcat
      901 cgcggactcc tttgcgacct cgaagctccg caggtccttc ttcgcgagag acagcagccg
      961 ctcgcgatcg tcgatgatct gcacggtgtg cgtgtcctcg ttgagacggc gtccgcgaat
     1021 cgttctgccg tcggccgtga cgacgcgaac gggccggttg atgggaagca gcgaggaggt
     1081 gggatcgcga accgtgcgct ccagcacgac agccgcgcgc gcgagcccga tgtcgctcag
     1141 gtcgggcgcc gaccgtgggc ctcttccctc cacgcggtgg cacgtcgtgc actccccctt
     1201 gccttgaaag atcgtgcggc cgcgggcggc gtcgccgact ttcacggagg cgctcgtgtc
     1261 gaacccggcg cgaatgaagg cgacgatgcc cgtcagctcg gcggcgtcca gcttgaatgg
     1321 cggcatcccg cccggcgtgc cccgcgtgat cacctgcgcg acgtcttcgt ccgaccgcga
     1381 gcggcggaag agaccgcgcc tgagatcgac gccggagacc tcctcgccga accggccatg
     1441 gcagtgagcg cactgcgcgg tgtacaggag gctgccgcgc gcgatgtcct cctggttgta
     1501 ctgacctggg tgatcgagag acagcaactg cgcgtccgcc ggcgcggcga gaacacacag
     1561 aacggcgatc gtcagcagg
//
LOCUS       DBNGSH010000432         2241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4284068, whole genome shotgun sequence.
ACCESSION   DBNGSH010000432 DBNGSH010000000
VERSION     DBNGSH010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4284068"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /gene="ilvA"
                     /locus_tag="AC16CY_09820"
     CDS             complement(<1..1160)
                     /gene="ilvA"
                     /locus_tag="AC16CY_09820"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HIR3354128.1"
                     /translation="MLVTAELTQLLRDVLTSRVYDVASETPLDAAPRLSERLDNEVLL
                     KREDLQPIFSFKLRGAYNRIAHLTDRERGLGVIAASAGNHAQGVAFSAKKLELRAVIV
                     MPQTTPRIKVDAVRSMGAEVVLAGDSYNEAQAHCDTLIAETGMTFIHPFDDPLVIAGQ
                     GTIGAEILRQSQQRLSAVFVPVGGGGLIAGIAGYVKSLRPDVKVIGVEPFEADAMYRS
                     MALGRRVQLEHVGIFADGVAVREVGERTFAIAQQTVDEIVRVTNDEICAAIKDVFDDT
                     RSVMEPAGALATAGVKAWVEREGARNQSIVALLSGANINFDRLRFVAERAELGESREA
                     LIAVTIPERPGAFREFCAAIGRRTVTEFNYRLSGRDQAHIFVGFQIQSRQDGA"
     gene            complement(1239..1982)
                     /locus_tag="AC16CY_09825"
     CDS             complement(1239..1982)
                     /locus_tag="AC16CY_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3354129.1"
                     /translation="MLKVELHTHTADDPVDLIPHSTHELIDRAAALGYQALALTLHNL
                     QLDPRPFVSYAAERGVTLIPGIERTIEGRHVLLLNFSTRSMDVDSFEALARLKAREPG
                     LVVAPHPFFPMGNCLRDMMDRHADLFDAVECNAMYTSTLNFNEKAERWARERGKPMVG
                     NGDVHRLRQLGTTWSMVDAEPTADAICDAIKAGRVRVESRPLSWVSAATTMSRLFSSD
                     LRTMIGHRQGAGDRGRGAEDTRHGAGVRG"
BASE COUNT          340 a          789 c          739 g          373 t
ORIGIN      
        1 gcgccgtcct gcctcgactg gatctggaag ccgacgaaga tatgcgcctg gtcgcgcccg
       61 ctgagccggt agttgaactc ggtgacggtc cgccgtccga tggccgcgca gaactcgcgg
      121 aacgcgccgg gccgctcggg aatggtgacg gcgatcagcg cctcgcgcga ctcccccagc
      181 tcggcgcgct cggccacgaa ccggaggcga tcgaagttga tattggcgcc gctcaacagc
      241 gccacgatcg actggttgcg cgccccctcg cgctccaccc acgccttcac cccggccgtc
      301 gccagcgcgc ctgccggctc catcacgctg cgagtgtcgt caaagacgtc cttgatggcc
      361 gcgcagatct cgtcgttggt cacgcggacg atctcgtcca cggtctgctg cgcgatcgcg
      421 aacgtccgct cgcccacttc acgcacggcg acgccgtcgg cgaagatgcc gacgtgctcg
      481 agctgcacgc gccggccgag cgccatcgat cggtacatgg cgtccgcctc gaacggctcg
      541 acgccgatga ccttgacgtc gggccggagc gacttgacgt agccggcgat gccggcaatc
      601 aggccgccgc cgccgacggg cacgaagacg gccgagagac gctgctggct ctgacggagg
      661 atctcggcgc cgatggtccc ctgcccggca atcacgagcg gatcgtcgaa gggatggatg
      721 aaggtcatcc cagtctcggc gatcagcgtg tcgcagtgcg cctgcgcctc gttgtagctg
      781 tcgccggcca gcacgacctc ggcgcccatc gaccgtaccg cgtccacctt gatcctcggg
      841 gtggtctggg gcatgacgat cacggcgcgc agctcgagct tcttcgcgga gaaggcgact
      901 ccctgcgcgt ggttgcccgc gctggccgca atcacgccga gtccgcgctc tctgtcggtg
      961 aggtgggcga tgcggttgta ggcgccccgc agcttgaagc tgaagatcgg ctgcagatcc
     1021 tcgcgcttga gcagcacttc gttgtcgagc cgctccgaga gccgaggggc cgcgtcgagc
     1081 ggcgtctcgc tcgccacgtc gtacacgcgg ctggtcagga cgtcgcggag aagctgcgtg
     1141 agttcggcgg tgacgagcac gagggaatct tatcctgtcg ctcttcccct gccccctgcc
     1201 ccctgccccc ctgccccctg cccccctgcc cctgccctct aacccctgac ccctgccccg
     1261 tgcctcgtgt cctctgcccc ccgccccctg tcccctgccc cctgcctatg accgatcatg
     1321 gtccgcagat cgctcgagaa gaggcggctc atggtcgtcg ccgcggacac ccaggagagc
     1381 ggacgggact cgacgcgcac gcggcccgcc ttgatcgcgt cgcagatggc gtccgcggtg
     1441 ggctcggcgt cgaccatcga ccacgtcgtc ccgagctgcc ggagtctgtg aacgtcgccg
     1501 ttgccgacca tcggcttgcc gcgctcacgc gcccaccgct ccgccttttc gttgaagttc
     1561 agcgtcgagg tgtacatcgc gttgcattcg acggcgtcga agaggtcggc gtgccggtcc
     1621 atcatgtcgc ggagacagtt tcccatcgga aagaacgggt gcggcgcgac gaccagcccc
     1681 ggctcgcgcg ccttgagacg cgcgagcgcc tcgaagctgt cgacgtccat gctgcgggtg
     1741 ctgaagttga gcagcaggac gtgccgtccc tcgatcgtgc gctcgatgcc gggtatgagc
     1801 gtgacgcctc gctccgcagc gtaggacacg aacggccgcg gatcgagctg caggttgtgc
     1861 agcgtgaggg cgagcgcctg atagccaagc gccgccgccc gatcgatcag ctcgtgcgtc
     1921 gagtgcggaa tcaggtcgac cggatcgtcg gccgtatgag tgtgcagttc gaccttgagc
     1981 acctcccgga cctactcaag atccttgccg ggcccatggg ccggacgggc ccgatcctgc
     2041 agggatttga ggggttcttg acgaagcggc ggggacgcgt ttccgagaaa acccagaaaa
     2101 aggtccgctt tgggaacgcg ggccttacga cgagatcgcc cggactgcca ggtatccgta
     2161 caagagcagc gcggcgccca gcaccgcgtt cacgacgaca ccgttgcgcg cggcgctcac
     2221 gtggcgcgag ttgttgagcc a
//
LOCUS       DBNGSH010000433         1509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3435350, whole genome shotgun sequence.
ACCESSION   DBNGSH010000433 DBNGSH010000000
VERSION     DBNGSH010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3435350"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="AC16CY_09830"
     CDS             <1..819
                     /locus_tag="AC16CY_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3354130.1"
                     /translation="PFVEVNCAAIPEELIESELFGHVRGAFTGAVADRRGRFEGAHGG
                     TIFLDEIGDMSLKTQAKVLRVLQEQVIQAVGGSALIRVDARVLAATNKDLPAEIRTGR
                     FREDLYFRLNVVPIFVPPLRERYEDVPLLADHFMAILAREYGRRPKTFDADAVAAMQR
                     YRWPGNVRELRNLVERLMIMVPGDRVSSRDLTFLEQGGAPEAVAAARPASFAPLHHAR
                     DEFEKQYILRALASQQGNMSRTAEALGVERSNLYRKMRAFGIMPARRGDDDEVA"
     gene            803..>1509
                     /locus_tag="AC16CY_09835"
     CDS             803..>1509
                     /locus_tag="AC16CY_09835"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3354131.1"
                     /translation="MTRLPEPAANAPGPRTRGAIPTPALLLDLAALDRNITRMAAFFA
                     RGTCRLRPHVKAHKTLEIARRQLAAGSCSGLTCATVSEAEMVAPICDDLLIANEIVSA
                     SKCERVAVLARRTRVTVAVDSLAGLDALSKAARAGGVRVGVLVDVNVGQNRCGVEPGE
                     AALTLARRAVQSDALDLRGVMGYEGHLQPVRDRTERESRTRHAMQGLLHAAGQFRAAG
                     FPCDVVSAGGTGTYDISG"
BASE COUNT          229 a          513 c          538 g          229 t
ORIGIN      
        1 ccgttcgtgg aggtcaactg cgcggccatt ccggaagagc tgatcgaaag cgagctgttc
       61 ggccacgtca ggggcgcgtt caccggcgcc gtggcggacc gccggggcag gttcgagggc
      121 gcccacgggg gcaccatctt cctcgatgaa atcggcgaca tgagcctcaa gacccaggcg
      181 aaggtcctgc gcgtcctgca ggagcaggtg atccaggcgg tcggcggctc ggcgctgatc
      241 cgcgtggacg cgcgcgtgct ggccgcgacc aacaaggacc ttcccgccga aatcagaacc
      301 gggcgcttcc gcgaagactt gtacttccgg ctgaacgtcg tgccgatctt cgtcccgccg
      361 ctccgcgagc gctacgagga cgtgccgctg ctggcggatc acttcatggc gattctcgcg
      421 cgggaatacg gccggcggcc caagacgttc gacgccgatg ccgtcgccgc gatgcaacgc
      481 taccggtggc ccggcaacgt ccgcgagctg cggaacctgg ttgagcgcct gatgatcatg
      541 gtgcccggcg atcgcgtctc gtcgcgggac ctgacgtttc tcgagcaggg aggagcgccg
      601 gaggccgtgg ccgcggcacg gcccgcgtcc ttcgctccgc tgcaccatgc gcgcgacgag
      661 ttcgagaagc agtacatcct gcgcgcgctc gcgagccagc agggcaacat gtcccgcacg
      721 gcggaggcgc tcggggtcga acgcagcaac ctttatcgca agatgcgcgc cttcggcatc
      781 atgccagccc ggcgcggaga cgatgacgag gttgcctgaa ccggcggcga acgcgcccgg
      841 gcctcgcacg cgcggcgcga ttccgacgcc ggcgctgttg ctcgacctcg ctgcgctcga
      901 tcgaaacatc acgcgcatgg cggccttctt cgcccgcggc acgtgccggc ttcgcccgca
      961 cgtcaaggca cacaagacgc tcgagattgc acggcgccag ctcgcggccg gctcgtgttc
     1021 tgggctgacc tgcgccacgg tctcggaggc ggagatggtc gcacccatct gcgacgatct
     1081 gctcatcgcc aacgagatcg tcagcgcatc gaagtgcgag cgcgtggcag tgctggcgcg
     1141 acgcacccgg gtcactgtcg cggtggattc gctcgcgggc cttgacgcgc tgtcaaaggc
     1201 ggcgcgagcc ggcggcgtcc gggtgggcgt gctcgttgac gtcaacgtcg gccagaaccg
     1261 gtgcggcgtg gagcctggcg aggccgcctt gacactggcc cggcgtgccg ttcagtcgga
     1321 cgcattggac ctgcgcgggg tcatggggta tgagggacac ctccagcctg tgcgcgacag
     1381 gaccgagcgc gagtcgcgaa cgcgccacgc catgcagggc ttgctgcacg cggccggcca
     1441 gttccgggcg gccgggtttc cgtgcgacgt cgtctcggcc ggcgggaccg gcacctacga
     1501 catcagcgg
//
LOCUS       DBNGSH010000434         2494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1227643, whole genome shotgun sequence.
ACCESSION   DBNGSH010000434 DBNGSH010000000
VERSION     DBNGSH010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1227643"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="AC16CY_09840"
     CDS             <1..1899
                     /locus_tag="AC16CY_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354132.1"
                     /translation="ALAAALHKLVHAAVPVNDDFMHLTYSRQLLAGEWPVRDFFDYGM
                     SLMYTISALAQLAFGYRLLSEGIVVAVMVGISTFLVYDISRRATGSTAAAVLAALLLV
                     VSVPRGYAYPKWIVYAAAASLWWRYVWEPGRGRALALGVWTAVAYYWRPDHGAYVAIG
                     VVSAMLAAHGVSALTLMRSLQIGVVAVALAAPSVVYSATQSGGIVAFVQSGINSAVGE
                     HLGTGQAIPRWTISRPSDILSVDPAEKYAPTIGIRWTKGSSAESRQQVIQQYGLTTVS
                     VRDDVTQSVRLSERSVRGLRELMGEPILEDTDGVDRAAGRVSSSTWPASQRWRFNWWW
                     LRVRVLPGLDDQAAAGEAATLLLIAVPLIAVGASVWLGQYLPVQVGRLPLAAFAVFAL
                     VVDFGLLRTPFSVRAAEAVALPAIAFGLLMATSFRIARIHHGWRRWPAGAAAAVLTLL
                     VLKSLAGAGQSGERIRWLAGDGNVERARIAWADASSRLGSSPPIEHWREQGGSTLRLA
                     VYAHDCLSSSDRIFVNWFAPDIYYYADRLMAGRQLFFPPVLGALAHERRMEQEKFERM
                     PAQMVFLRSMADNAAEKTFPEFVEIFARDYRVAGVIGDDGERYSILVRKDRVPVRTYR
                     DSGWPCYR"
     gene            1974..>2494
                     /locus_tag="AC16CY_09845"
     CDS             1974..>2494
                     /locus_tag="AC16CY_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3354133.1"
                     /translation="MGIYRFVLAALVVLFHFGGLGWIVGRMAVFAFYSVSGFLIFQVL
                     DRVYLNEPRGSWRFLFNRFVRLAPLYLAYTLLTLLMVRLLGPASYLNPGGGSAILAGM
                     DRSTLDLLFNGTTLAPRLAIDGFMPVLVFEPLLIPQGWSIGVEASFYLLAPLVVLSTR
                     RRTWGMAIWIAAGV"
BASE COUNT          323 a          842 c          833 g          496 t
ORIGIN      
        1 gcgctcgcgg cggcgcttca caagctcgtg cacgccgcgg taccggtcaa cgacgacttc
       61 atgcacctga cgtactcgcg tcagctgctt gccggcgagt ggccggtccg cgacttcttc
      121 gattacggca tgagcctgat gtacacgatc agcgctctgg ctcagctcgc cttcggttac
      181 cgcctcctgt cggagggcat cgtggtcgcc gtgatggtgg gtatcagcac ctttctcgtc
      241 tacgacatca gccgccgcgc caccggctcc accgcggccg ccgtcctggc ggccctcttg
      301 ctcgtcgtct ccgtgccgcg cggctacgcg tatcccaagt ggatcgtcta cgccgcggct
      361 gcgtccttgt ggtggcgcta cgtctgggag ccgggccgcg gcagggccct ggccctgggc
      421 gtctggacgg cggtcgccta ctactggcgt ccagaccacg gcgcctacgt cgccattggt
      481 gtggtttccg cgatgcttgc ggcacacggc gtcagcgccc tcaccctgat gcgatctctc
      541 cagatcggcg tcgtcgctgt cgcgctggcg gcgccgtccg tggtgtactc agcgacgcag
      601 agcggcggga tcgtggcgtt cgtccaaagc gggatcaact ccgccgtcgg ggaacacctc
      661 ggcacggggc aggccattcc gcgatggacg atttctcgcc cgagcgacat tctcagcgtc
      721 gatccggccg agaagtacgc accaaccatc ggcattcgct ggacgaaggg cagttcggcc
      781 gaatcgcgcc agcaggtgat ccagcaatac ggcttgacga ccgtatcggt ccgggacgac
      841 gtcactcaga gcgtgcgcct ctccgagcga tcggtccgcg ggttgcgcga gctgatgggc
      901 gagccgatcc tcgaggacac cgacggcgta gaccgggcgg cgggccgcgt gtcttcctcg
      961 acctggccgg cctcgcagcg atggcgattc aactggtggt ggctcagggt cagggtgctg
     1021 ccgggactcg acgaccaggc cgcggcaggc gaagcggcca cccttctgct gatcgcggtg
     1081 ccgctcattg cggtgggtgc gtccgtgtgg ctcggtcagt acctgccggt ccaggtcggg
     1141 cgtctgccgc tggcggcgtt cgctgtgttc gccctggtcg tcgatttcgg actgctcagg
     1201 acgcccttca gcgtccgtgc ggcagaggcc gttgcgctgc ccgcgatcgc ctttggcctg
     1261 ttgatggcga cgtctttccg gatcgcgcga atccaccacg gatggcggcg ttggccggct
     1321 ggtgcggcgg cggccgttct cacgctcctg gtgctcaaaa gcctcgccgg cgcgggtcaa
     1381 tccggcgagc gaatccgctg gctggcgggc gacggcaatg tcgagcgggc ccggatcgcc
     1441 tgggccgacg cctcgagccg gctcggatcg tcgccgccca tcgagcactg gcgggagcag
     1501 ggagggagca cgctgcgcct cgcggtctac gcccacgact gcctgtcctc gtccgaccgg
     1561 atcttcgtga actggttcgc tccggatatc tactactacg ccgatcggct catggccgga
     1621 cgtcaactgt tctttcctcc cgtcctcggc gcgctggcgc acgagcggcg gatggaacag
     1681 gagaagttcg aacgaatgcc cgcacagatg gtcttcctga gatcgatggc cgacaacgcc
     1741 gcggagaaga cgtttccgga gttcgtcgag atcttcgctc gtgactatcg cgtcgcgggc
     1801 gtcatcggcg acgacggcga acgctattcg attctggtca ggaaggatcg cgtgccggtc
     1861 cgcacgtacc gcgactccgg gtggccgtgc taccggtagg ccctgccccc tgcccctgcc
     1921 ccctgccccc tgccccctga tccctgcttc agctatcctg tggcggccgg gcgatgggaa
     1981 tttaccggtt cgtgctcgcg gccctcgtgg tgctctttca ctttggaggg ctgggctgga
     2041 tcgtcggccg gatggccgtc ttcgccttct actcggtcag cgggttcctc atctttcagg
     2101 tgctcgaccg cgtctacctg aacgagccgc gcgggtcctg gcgcttcctc ttcaaccggt
     2161 tcgtgcggct ggcgccgctg tacctcgcct acacgctgct gacgcttctg atggtccggc
     2221 tgcttggtcc tgcgtcgtat ctgaatcccg gcggagggag cgcgattctg gccggaatgg
     2281 atcgaagcac gctcgatctg cttttcaacg gcacgacgct cgctccccgg ctcgcgatcg
     2341 acggcttcat gccggtcctc gtattcgaac cgctgctgat tccccaggga tggtcgatcg
     2401 gggtcgaggc gtcgttctac ctcctcgcgc cgctcgtggt gctctcgacg agacgccgca
     2461 cctggggcat ggcgatctgg attgcggctg gggt
//
LOCUS       DBNGSH010000435         2709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_40403, whole genome shotgun sequence.
ACCESSION   DBNGSH010000435 DBNGSH010000000
VERSION     DBNGSH010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_40403"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..803)
                     /locus_tag="AC16CY_09850"
     CDS             complement(288..803)
                     /locus_tag="AC16CY_09850"
                     /inference="COORDINATES: protein motif:HMM:NF023123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HIR3354134.1"
                     /translation="MAARIAFFTLGVLKAPIGDGVVQGFVDRLASVYAAAEGSAGFFA
                     RSIRDVQTWEHSWGPVLAPTCVPADLGLNQLAMTLSLWRDLESVAAFAYRGVHAEALS
                     KRGDWFERGPWPSFVAWWVEEHHQPNWTEAVERIDRLHAQGSTPAAFNFRQPFDSAGK
                     PTKLNRALVGP"
     gene            complement(932..1894)
                     /locus_tag="AC16CY_09855"
     CDS             complement(932..1894)
                     /locus_tag="AC16CY_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354135.1"
                     /translation="MTALIVLVALAGSPASSQTRTPWGDPDLQGTWDFTTITALQRPP
                     ELAGKEFLTEREAAALEREINQRRFDTEADSPGSLGGTPRPATDPGIYNLGWWWEPNG
                     RKLVGTRRTSLVIDPPDGRLPALTPLAQKTQRAREQARERAAGPADLPLAERCVMGFN
                     SGPPMVPGPYNNLVQLFQSPGYVVIVNEMVHDARIVPLGKSAHVGDGIRLWMGDSRGR
                     WEGNTLIVETRNFTHHGTGTFGPPGLVDRNLHLVERFTRLDDRRLLYQFTIDDPTVWT
                     RPWTAEVPMTRVDGFVMEYGCHEGNYGLANILSGARADERVESR"
     gene            complement(1924..>2709)
                     /locus_tag="AC16CY_09860"
     CDS             complement(1924..>2709)
                     /locus_tag="AC16CY_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3354136.1"
                     /translation="AGAGFRGSLEGWARAGIKYVELTNTLLDGFLKSDSLPAARRVVT
                     DLGLTPVCCACGVFGLWEPHPKRAALLDEFRKRCEQFAALGLTRIYSPTPTTERFTED
                     DYKTGVENMREVGNIGKQFGMSVSVEATRSSTFIASLPTMLRMTRAAGNVSPLLDFYH
                     FWSGPNKLADLDAIRPGEIGHVHFQDVPDMPRELLDQTTRVIPGDGVAPVVGILRKLD
                     EKKYTGPLSVELFLPKFQQGNAFEIAREIRQKSETVMRRAKVL"
BASE COUNT          453 a          921 c          876 g          459 t
ORIGIN      
        1 agattcagcc cgatgccgac ggccagtacg agcgtcgccg acgcagcgaa gccgggattc
       61 cgccagagcg cgcgagcgcc gtagcgaatg tcgtgcacca ggtcgtccat ccatcgtctg
      121 aagcgggaca tgcaggcctt tcgggagggg cggcgcccct cgatgcgttc agcgcgattc
      181 tacgcgctca tcggcgcgga cccgttcatg atcagcgtgg gagttggccc gccgagatcg
      241 ccacgaccat cgaaacggcg ccgcgcacac acagctgagc gccggcgtca cggcccaacg
      301 agcgctcggt tcaacttggt gggcttgccc gctgagtcga agggctgccg gaagttgaac
      361 gccgcgggcg ttgagccttg cgcatggagt cggtcgatgc gctcgacggc ttcagtccag
      421 ttcggctggt gatgttcttc gacccaccaa gcaacgaagc tcggccacgg gccacgctcg
      481 aaccagtccc cgcgcttgga gagggcctca gcgtggacgc cccgatacgc gaaggcggcg
      541 accgactcga gatctcgcca caggctcaac gtcatcgcga gctggttgag tccaaggtcc
      601 gccggcacgc acgtgggcgc aagcaccgga ccccacgaat gctcccaggt ctgcacgtcc
      661 cggatggatc gtgcgaagaa cccggcgcta ccctcggcag ccgcataaac ggacgccagt
      721 cggtccacaa aaccttgcac aacgccgtcg ccaataggcg ccttcaggac cccgagtgtg
      781 aagaaggcaa ttcgagcggc cacgcgtgca cattagcatc gaaggcgact gctcgggaac
      841 tcctgagccg cacattcccg caggaggcga atcgctctgt cgccctgacg tctcatcgag
      901 tagaccgaca cgagccttct ggctaggaaa gtcagcgcga ttctacgcgc tcgtcggcgc
      961 gcgcgccgct gaggatgttt gccaggccgt agttgccttc atggcagccg tactccatga
     1021 cgaagccgtc aaccctcgtc atcggaacct ccgccgtcca gggacgcgtc cacaccgtgg
     1081 gatcgtcgat cgtgaactga tacaggagcc ggcggtcgtc gaggcgggta aaccgctcga
     1141 ccaggtgaag gtttctgtcc accagcccgg gagggccgaa cgtgcccgtg ccgtggtgcg
     1201 tgaagttcct cgtctccacg atcaacgtgt tcccctccca tcgcccgcgc gagtccccca
     1261 tccagagccg gatgccgtcg ccgacatgcg cggacttgcc gagcggaacg atgcgcgcgt
     1321 cgtgcaccat ctcgttgacg atgacgacgt agccgggact ctggaacagc tggacgagat
     1381 tgttgtaggg gcccggcacc atcggcggtc ccgagttgaa tcccatgacg cagcgttcgg
     1441 cgagagggag atcggcgggg ccggccgcgc gctcgcgggc ctgctcgcgg gcgcgctgcg
     1501 tcttctgcgc gagcggcgtc aacgccggca gccttccatc cgggggatcg atgacgagcg
     1561 acgtccgcct ggttcccacc agcttcctgc cattcggctc ccaccaccac cccaggttgt
     1621 agatgccggg atcggtcgca ggacgcggcg tcccgccgag cgacccggga ctatcggcct
     1681 cggtgtcgaa ccgacgttga ttgatctcgc gctcgagggc tgccgcctcg cgctcggtca
     1741 ggaactcctt tcccgcgagc tcgggcgggc gctgcagcgc cgtgatggtc gtgaagtccc
     1801 acgtgccctg caggtccggg tctccccagg gcgtgcgcgt ttgactcgag gctggcgagc
     1861 ccgccagcgc gacgagcacg atcaacgctg tcactccagc ctttcgcatc ctctccctgc
     1921 cccctacagc acctttgctc tccgcataac ggtctccgac ttctgtcgga tctcccgtgc
     1981 aatctcgaac gcgttccctt gctgaaactt cggcaggaac agttcgaccg agagcgggcc
     2041 cgtgtacttt ttctcgtcga gcttgcggag gatccccacc accggtgcaa cgccgtctcc
     2101 cggaatcacg cgggtcgtct ggtcgagaag ctcgcggggc atgtcgggca cgtcctggaa
     2161 atgcacgtgc ccgatttcgc cggggcggat cgcgtcgagg tccgcgagtt tgttcgggcc
     2221 ggaccagaag tggtagaagt cgagcagcgg cgacacgttc cccgccgcgc gcgtcatcct
     2281 gagcatggtc ggcagcgacg cgatgaacgt cgagctgcgc gtggcttcga cggacacgct
     2341 catgccgaac tgcttgccga tgttccccac ctcgcgcatg ttctccacgc ccgtcttgta
     2401 gtcgtcctcg gtgaatcgtt ccgtggtggg cgtgggcgaa tagatgcgcg tcagcccgag
     2461 cgcggcaaac tgctcgcacc gcttcctgaa ctcgtcgagc agcgccgcgc gcttcgggtg
     2521 tggctcccag aggccgaaca cgccgcaggc gcagcagacg ggtgtcaggc cgaggtcggt
     2581 caccacgcgc ctggcggcgg gcagcgaatc ggatttcaga aacccgtcga gcagcgtgtt
     2641 cgtgagctcg acgtacttga tgccggcgcg cgcccaccct tcgagcgatc cgcggaagcc
     2701 ggcgccggc
//
LOCUS       DBNGSH010000436        11751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1907871, whole genome shotgun sequence.
ACCESSION   DBNGSH010000436 DBNGSH010000000
VERSION     DBNGSH010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11751
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1907871"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="AC16CY_09865"
     CDS             complement(<1..243)
                     /locus_tag="AC16CY_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HIR3354137.1"
                     /translation="MSNTSRVPPPVNDINTTYLPGSPERAELKARLAQMSAERIEIPI
                     VIDGCAIRTGRVEPAVMPHDHRHVLGDWHAADPSHVQ"
     gene            317..3031
                     /locus_tag="AC16CY_09870"
     CDS             317..3031
                     /locus_tag="AC16CY_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3354138.1"
                     /translation="MLIAALAAPAGHAAQDAQRSTETFQSRVDLIALDVAALDGSGRP
                     VEDLRLRDFVVKIDGRERPVVSASLVKVERGAPTAPAAAPASPLVTTNVTPANARRVV
                     IAVDQTLIAPKAITPLLNTASKFVDGLTPIDYAAFLAFPEPGPRVDFTTDKALVRKAL
                     QGFVGQPRQQRLKLFDISTTEAFEVANKERSTRGETVGPTTSRILERGCTTVVIVCLL
                     QIEMQALQMVQDFRAEATTSLRRLESFLTELIPIEGSKTLVLVSAGLVVEDHSLLNEV
                     VRLAALARTSINVIAVEPERDEEIRDQPNSQPKTTLQDRSFEQQGLETAADGSGGFFV
                     RASGGRSEGIFDRVANEMSAWYVVAVNRQPGDLERQTVDVQVKRRGVTVRMNRRFVTA
                     PAAVRRPGEEVLREALSSPIAISGVPLRVSTFAQRDGAAGKYRLHLAAQVGQPGTPPR
                     EYSVGYVVMNEQNRPVTSLGRRLQLAPAAGGGPNEPLHFDTALLIDPGAYSLRFGVVD
                     PDGRRGTVIRRVELEPLPGEGLRTSDLIIGNAVAEGELLHPSVEPRVRGQMTGYLELY
                     LPQADPGALTVALEIAEGEASPALATKMLNVAGGSQPDWRVASGIVDVNVLPGKYVAR
                     ASIRRDGKAVHTVSRPFVLERTDPAERRQAERRQTEKPAVPITPEMQRQTASYVAQVV
                     GNLMNVVAQEDFELTSPDRRIRSEFLLVPYPGSNRDLLSFRHVTHVNGKPAPNREERL
                     AELFQKPIDLVLNRVRQIRLAAVNYVPSVFDPILGLAFLQANYQGRFEWTVNDAGAEW
                     QPQVKAVTFVERARPTLLRNWPLGDQDVPARGTAWIEQGTGRLLQSELQIGSGRSATK
                     ALTRFKVDEHLQIMVPDTMRTENPKGVATYSNFSRTGIDTRFVLPDPK"
     gene            3125..5920
                     /locus_tag="AC16CY_09875"
     CDS             3125..5920
                     /locus_tag="AC16CY_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3354139.1"
                     /translation="MRTHLACLMLIAALAAPAGRAAQDAQRSTETFQSRVDLIALDVV
                     AVDGGGRPVEDLRARDFTVKVDGRERPVVSAELVKVERGQPGEVPVLLPEGFVTTNVA
                     TANSRRVAVAVDQTLITPGSIAPLLRSASGFVEKLTPQDYAALITFPEPGPRVDFTTD
                     KARVRKGVQDVVGQPAKLTTGFFTISLTETVDIADKERVQLSSPPTWDTAGPTMRRVM
                     ERGCRGRSLDELMMPENAMELEQCLRDLANQSVTDALIARQDGNISLRRLESFLTELI
                     PIEGPKSMILLSAGMLVENLQLLDEAARLAAAARTSISVIAVELEREQEIKSLPNSQG
                     STKLFDRTLELQGLETVADRTGGTFTRFRAGTGEGIFDRLESAMSAWYIVAVERRPGD
                     PERQRIDVEVKRKGVTARTSRMVVSTSAVNASRPKEEVLRDALSSPIAVSGVPLRVST
                     FAQRDATMGKYRLHIAAQVGQPGSPSVEYSVGYVVMNADNRPVTSLGRRLQLSPAPGG
                     GSSESLHFDTALLIDPGTYSLRFGVVDPDGRRGTVVRRVELEPLPGEGLKTSDLIVGD
                     VVAEGDVLHPSVEPHVRGQVASYLELYLPDADPGGLTVALEIAEGEASPALATKLLNI
                     GAGSQPDWRVATGIVDVSVLPGKYVARASIRRDGKAVRTVSRPFVLEKTDPAVRRQTD
                     RRQNEKPGVPITPEMQRQTASYVAQVVGNFTNVMGTEDFALKGPDRRVTSNVLLVPYP
                     GSQRDLLFFRNVTQVNGVAVPDRLERLSDLFQKPVALVRDRVRQITLAAENHVPPIFN
                     PIFVLAFLQADLQGRYEWTVTDAGAEWPTQVKAVTFVERARPTVLRAGPLGDQDVPTR
                     GTAWIEEGTGRVLQTELQVGSGRSTIKALTKFKLDDRLQIMVPDTMRTENPSGVATYS
                     DFERFSVETQFVVPDPK"
     gene            complement(5931..7325)
                     /locus_tag="AC16CY_09880"
     CDS             complement(5931..7325)
                     /locus_tag="AC16CY_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3354140.1"
                     /translation="MAEPRGAQRKFDRSIVEGPIGPAVWKLAWPTMLQNVIAGLQGII
                     DHAMVGHFVGYTANAAIGVSWQIILVVIVFISSLFTGMAVLVARFAGANEPGKVNRVV
                     YQAFLTAFALSVVLAAVGYLAAPSLLGLVNAAPDVQAEALPFLRAMFVGIFGMMMFFM
                     LSGAFRAAGDPQTPLRLGVVMTVLTIVFNVVLIPIFGTVGAAFGTIASSTSVSVYGLW
                     QLTRPKSVIHFERGMDLRPDFGIVRSLFRFGLPTGVQGIAMNIGGVLLLRFIGSLEHS
                     AAAQAAYAVGYTELFSLITWTSVGLMGASATIAGQNLGAGNAERAMEGVRVASRYGLG
                     VAAVVGAMFLLIPGYLLAIFGMTDPLVLSLGRTLLGFLTVSGFFITVALSYTGGLQGT
                     GDTRSPLYISIVSQVIVPLGLCAMLESLYGLEAVHIWTAIVIGHATRATLSVLRFRQG
                     KWRGIAVDIEPARS"
     gene            7389..8354
                     /locus_tag="AC16CY_09885"
     CDS             7389..8354
                     /locus_tag="AC16CY_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354141.1"
                     /translation="MTHRRCASVSLAIAVAIVWVWSANAVAQAPALSPRAASAAPAWS
                     HGRTPDGQPDLQGIWINFDSTPFETPAVQPAAQPAPAAGGAAAAARVGPASEFADHNH
                     KISARRASMVIDPPDGRVPVMKWAEDRRDYDLAHIPDAPEHETPWVRCITRGYPAGMF
                     PAGYNNAYQIIQIPGYVVMVFEMIHETRIIPIDGRPRLGEKIRQWNGEPRGRWEGNTL
                     VVETTNYNNKGSIATSAATGRMRGIPQTEAMRVVERFTRIDANTINYSVTIDDPKVYT
                     RPWTVSLPLNRDDSYEMFEYSCHEGNYAMANALSFGRVRDKEAGQ"
     gene            8556..9011
                     /locus_tag="AC16CY_09890"
     CDS             8556..9011
                     /locus_tag="AC16CY_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354142.1"
                     /translation="MSPDGTITAWVRQDFNIDPPDDHIYIRRQGHPVRVIKDLPPDGE
                     WCRVIVWSPDSTKVGFVINDARLDIFDVTHDRWLAPVALVFDGYNEARRVQFSPDGRA
                     ILYDVVQRAVVRLTYRDGRVIEAPLSRLSDKSRSSIREEVVLGAARVEL"
     gene            9239..10480
                     /locus_tag="AC16CY_09895"
     CDS             9239..10480
                     /locus_tag="AC16CY_09895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR3354143.1"
                     /translation="MRIRELALIGFVSVVSGWTVAAQRGPAPAPAGQEAIVPFRIQVP
                     DAVLSDLRQRLARTRFPGEIAGSEWDYGTNLGYLKELVAYWREKFDWRAAERRLNQFD
                     QFTTKIDGLDIHFIHQRSKDPKALPLAVTHGWPGSVAEFTKIIGPLTNPAAHGGSAAD
                     AFHVVAISLPGFGFSGKPAERGYGPERIAGIIAKLMARLGYTRYGLQGGDWGSAISRF
                     AALNDASHVAGLHVNFCLAGPPPGVKDPNEGVTPVELERSRARQAFFETERGYFLEQS
                     TKPQTIGSALDDSPAGLAAWIVEKFRSWSDSDGNVEKSFTKDDLLTNITIYWVTQTAT
                     SSARIYYENQRAKPAQGRVQVPTACAVFPKEISIPPRRWVEAQYNVTRWTEMPRGGHF
                     AALEEPELLVEDIRAFFRMLR"
     gene            10505..11221
                     /locus_tag="AC16CY_09900"
     CDS             10505..11221
                     /locus_tag="AC16CY_09900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3354144.1"
                     /translation="MRYQLLEWDSDFFGIGVARIIPARLSREELTETLTQLQQQRVTL
                     AYWFADQEFDHDVITSLGGVFVDRKTTFVVDLRTLHFDRIPLADRVEPFGDPMPVADL
                     ESLAVQSGEFSRFAVDPNLPKDKFVALYKVWIDRSIRKDLASEVLVIRDRDTIAGMVT
                     LGEKNARGAIGLIAVDSRYRGRKYGEALVRAAQRWFATHGYEYGQVVTQGQNVAACYL
                     YEKCGYSVERVECVYHFWIP"
     gene            11218..>11751
                     /locus_tag="AC16CY_09905"
     CDS             11218..>11751
                     /locus_tag="AC16CY_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354145.1"
                     /translation="MTVCVRACASVILVGAAALAVSTQGFHAFQGPPSIARPGATRMS
                     SNRVSSTVAASYVAREGRLVLLVLWRGSPAWLERGGGDSSRGGGASGRTWLQDAIGGV
                     TLDVEIDDATRVATVLGHRVPLEDGNVVLVDRVDTEPAVLRVLHVDPAVPLTPDAMYV
                     VMKREPELVEFLQCDLFR"
BASE COUNT         2021 a         3809 c         4012 g         1909 t
ORIGIN      
        1 ctggacgtgc gacgggtccg cggcgtgcca gtcgccgagg acgtggcgat ggtcgtgcgg
       61 catgacggca ggctcgacgc ggccggtccg gatggcgcac ccgtcgatga cgatgggaat
      121 ctcgatccgc tcggccgaca tctgtgcaag acgtgccttc agctccgccc gttcgggcga
      181 acctggcagg tatgtcgtgt tgatgtcgtt gacgggcggg ggcacgcgtg aggtattcga
      241 catggcccga tcttacgaca cggtccccgc gataaactgg agtcggcatt ctctgcggac
      301 tcatctcgcc tgtctcatgc tcatcgcggc gctcgcggcg ccggccggcc acgcggcgca
      361 ggacgcgcag cgctcgaccg agacgttcca gtcgcgcgtc gatctgatcg cgctggacgt
      421 cgccgccctc gacggcagcg gacggcctgt cgaggacctt cgcctacgag acttcgtcgt
      481 caagatcgac ggccgggagc gcccggtcgt ctcggccagc ctcgtcaagg tcgagcgcgg
      541 cgcgccgacg gcgccggcag cagctcccgc cagtccgctc gtgacgacca acgtcacgcc
      601 ggccaacgct cgccgcgtgg tgatcgctgt cgaccagacg ctcatcgccc cgaaagcaat
      661 cacgccgctc ctcaatacgg catcgaagtt cgtcgacggg ctgacaccga tcgattacgc
      721 ggcgtttctc gccttccccg agccggggcc gcgcgtcgat ttcacgaccg acaaggctct
      781 ggtgcgcaaa gccctgcagg ggttcgtggg gcagcctcga cagcagcgcc tgaagttgtt
      841 cgacatctct acgacggagg cgtttgaggt ggccaacaag gagaggtcga cgcgcgggga
      901 gacggtcggc ccgacgacga gtcggattct ggagcgcggt tgcacgaccg tagtcatcgt
      961 ctgcctcctg cagatagaga tgcaggcact gcagatggtg caggactttc gggcagaggc
     1021 gaccacctcg ctgcgccggc tggagtcgtt cctcaccgag ctcattccaa tcgagggctc
     1081 caagacgctc gtcctcgtgt cggcgggtct cgtagtcgaa gaccactcgc tcctgaacga
     1141 ggtggtccgg ctcgcagctt tggcgcgcac gtcgatcaac gtcatcgccg tcgaaccgga
     1201 gcgtgacgag gaaatcaggg accagcccaa cagtcagccg aaaacgacgc tccaggatcg
     1261 gtcgttcgag cagcaggggc tggagaccgc ggccgatggg agcggcggtt tcttcgtccg
     1321 cgcgagcggg gggcgcagcg aagggatctt cgaccgggtc gcgaacgaga tgtccgcctg
     1381 gtacgtcgtg gccgtcaacc ggcagcctgg agatctggag cggcagacgg tcgatgttca
     1441 agtgaagcgg cgcggcgtga cggtgcgaat gaaccggagg ttcgtgaccg cgccggccgc
     1501 ggtcagacgc cccggagagg aagtcctccg cgaggcgttg tcgtcgccga tcgcgatttc
     1561 gggggtcccg ctgcgcgtgt cgacgtttgc gcagcgcgac ggcgcagccg gcaaataccg
     1621 attgcatctg gcggcgcagg tcggacagcc gggcacgccg ccccgcgagt actcggtcgg
     1681 ctacgtggtg atgaacgagc agaaccgccc ggtgacctcg ctcgggaggc gcctgcagct
     1741 cgcgccggca gccggtggcg gaccgaatga gccgctgcac ttcgacacgg cattgctcat
     1801 cgacccgggc gcctactctc tgcgcttcgg cgtcgtcgat ccggacgggc gccgcggcac
     1861 cgtgatacgc cgtgtcgagc tcgagccgct gccgggcgag ggcctgagga caagtgacct
     1921 gatcatcggc aatgccgtgg ccgagggcga gctgctgcat ccgagcgtcg aaccgcgcgt
     1981 ccgcgggcag atgaccggct acctcgagct ctatctgccc caggccgatc cgggcgcgct
     2041 cacggtcgct ctcgagattg ccgaaggcga ggcctcgccg gcgctggcga cgaagatgtt
     2101 gaacgtcgcg ggcggatcgc agccggactg gcgcgtggcc tcgggcattg tcgatgtgaa
     2161 tgttctgccg ggcaagtatg tcgcccgcgc gagcattcgc cgcgacggca aggccgtcca
     2221 cacggtctcg cgcccgttcg tgctcgagcg gaccgatccg gccgagcgcc gccaggcgga
     2281 gcgccgccag accgagaagc ctgccgtccc gatcactcct gagatgcagc ggcagacggc
     2341 gtcgtatgtc gcccaggtcg ttggcaacct gatgaacgtg gtggcgcagg aggacttcga
     2401 gttgacgagt ccggaccggc ggatcagatc ggagttcctg ctggtgccat acccggggtc
     2461 caatcgcgat ctgctttcgt ttcgccacgt aacccacgtc aacggaaagc cggcgccgaa
     2521 ccgcgaggag cggctcgcgg agctgtttca gaagccgatc gatctggtgc tcaaccgcgt
     2581 gaggcaaatc aggctcgcgg ccgtgaacta cgtgccgtcg gttttcgatc cgattctcgg
     2641 gctcgcgttc ctccaagcca actatcaggg gcgcttcgag tggaccgtga acgacgccgg
     2701 cgccgagtgg cagccccagg tgaaagccgt taccttcgtc gaacgggcca ggccgacgct
     2761 gctgcgcaac tggccgctgg gcgatcagga cgtcccggcg cgcgggaccg cgtggatcga
     2821 acaggggacg gggcgtctgc tgcagagcga gctgcagatc ggcagcggac ggtctgcaac
     2881 gaaggctctg acgcggttca aggtggacga gcacctgcag atcatggtgc ccgacacgat
     2941 gcgcaccgag aacccgaaag gggtagcgac gtacagcaat ttcagccgga ccggaatcga
     3001 tacgcggttc gtgctcccgg atccgaaatg aggcgtcgcg cacgtatggt gccccccagt
     3061 cgccttctcg tcttgacggc ctcacgtcac gctcccccgc gatagactgg agcgcggtac
     3121 tcgtatgcgg actcacctcg cctgtctcat gctcatcgcg gcgctcgcgg cgccggccgg
     3181 ccgcgcggcg caggacgcgc agcggtccac cgaaaccttc cagtcgcgcg tcgatctgat
     3241 cgcgctcgat gtcgtcgccg tcgacggcgg cggacggcct gtcgaggacc ttcgcgcgcg
     3301 cgatttcacc gtcaaggtgg acggccggga gcgcccggtc gtgtcggcgg agctcgtcaa
     3361 ggtcgagcgc ggccagccgg gagaagtacc ggtgctcctc cctgaggggt tcgtcacgac
     3421 gaacgtcgcc acggcgaaca gccgtcgtgt cgcggtggcc gtggaccaga cgctgatcac
     3481 cccgggatcc atcgccccgt tgctccgttc ggcgtccggg ttcgtagaga aactgacgcc
     3541 gcaggactac gcggcgctga tcacgttccc ggaaccggga ccgcgcgtgg acttcacgac
     3601 cgacaaggcg cgcgtgcgca agggcgtcca ggacgtcgtc gggcagcctg cgaagctgac
     3661 gaccgggttc ttcaccatct cgctgacgga aacggtggac atcgcggaca aggaacgcgt
     3721 ccaactcagc agccccccca cttgggacac ggccggaccc acgatgcgtc gcgtcatgga
     3781 acgcggctgc cgcgggcgtt ccctcgatga gctcatgatg cccgagaacg cgatggagct
     3841 cgagcagtgc ctccgcgacc tggccaacca gtcggtgacg gacgcgctga tcgcccggca
     3901 ggacgggaac atttcgctgc gccggctgga gtcattcctc accgagctca tccccatcga
     3961 aggaccgaag tcgatgattc tcctctcggc gggcatgctc gtcgagaacc ttcagctcct
     4021 cgacgaggcg gcccggctgg ccgccgccgc gcggacgtcg atcagcgtca tcgcggtcga
     4081 gttggagcgc gagcaggaga tcaagagcct gcccaacagc caggggtcga cgaaactgtt
     4141 cgatcggacg ctcgagctgc aggggctcga gaccgtggcc gacaggaccg gcggaacgtt
     4201 cacgcgcttc agggccggca ccggcgaggg catcttcgat cggctcgaga gcgcgatgtc
     4261 ggcgtggtac atcgtggccg tcgagcggag gcccggcgac ccggagcggc agcgcatcga
     4321 cgtcgaggtg aagcgtaagg gcgtgacggc ccgaacgagc cgcatggtcg tgtcgacgtc
     4381 ggcggtcaac gcgtcccgcc cgaaggaaga ggttctgcgt gacgcgttgt cgtcgcccat
     4441 cgcggtgtcg ggggtgccgc tgcgcgtctc gacctttgcc cagcgggacg ccacgatggg
     4501 gaaatatcgc ctgcacatcg ccgcccaggt cgggcagccg ggctcgccgt cggtcgagta
     4561 ctcggtcggc tacgtggtga tgaacgcgga caaccgcccg gtcacgtcgc tcgggaggcg
     4621 cctgcagctc tcgcccgcgc ccggcggcgg atcgagcgag tcgctgcatt tcgacacggc
     4681 attgctcatc gacccgggca cctactccct gcgcttcggc gtcgtcgatc cggacgggcg
     4741 ccgcggcacc gtggtacggc gtgtcgagct cgagccgctg ccgggcgagg gcctgaagac
     4801 gagcgacctg atcgtcggcg atgtcgtggc cgaaggcgac gtcctgcatc cgagcgtcga
     4861 gccgcacgtc cgggggcagg tcgccagtta cctggagctc tacctgccgg acgccgatcc
     4921 gggcggactc acggtcgctc tcgagattgc cgagggagag gcctcgccgg cgctggcgac
     4981 gaagctgttg aacatcgggg ccggatcgca gccggactgg cgcgtggcca cgggcatcgt
     5041 ggacgtgagc gtcctgccgg gcaagtatgt cgcccgcgcg agtattcgcc gcgatggcaa
     5101 ggccgtccgc accgtctcgc gccccttcgt gctcgagaag accgatccgg ccgtgcgccg
     5161 ccagacggac cgccgccaga acgagaagcc cggcgtcccg atcacgcccg agatgcagcg
     5221 gcagacggcg tcctacgtcg cccaggtcgt cggcaacttt acgaacgtga tgggcacgga
     5281 agacttcgcg ctcaagggcc ccgatcggcg ggtgacgtcg aacgtcctgc tggtccccta
     5341 tccgggctcc cagcgcgatc tactcttctt ccgtaatgtc acgcaggtca acggagtggc
     5401 ggtgccggac cgcctggagc ggctctcgga tctgttccag aagccggtcg cgctcgttcg
     5461 cgaccgcgtc cggcagatca cgctcgcggc cgagaaccac gtgccgccga ttttcaaccc
     5521 gatcttcgtg ctggcattcc tccaggccga cctgcagggg cgctacgagt ggaccgtgac
     5581 cgacgccggc gccgagtggc cgacccaggt gaaagcggtc accttcgtcg aaagggcgag
     5641 gccaacggtg ctgcgcgcgg ggccgctggg cgatcaggac gtaccgacgc gcggcaccgc
     5701 gtggatcgaa gaagggactg gtcgtgtgct tcagaccgag ctgcaggtgg gcagtggacg
     5761 ctcgacgata aaagcgttga cgaagttcaa gctggatgac cggctgcaga tcatggtgcc
     5821 cgacaccatg cgcacggaga acccgtccgg cgtagcgacc tacagcgact tcgagaggtt
     5881 ctcggtcgag acgcagttcg tggtgcccga tcccaagtga actgtgtgca tcaggatctg
     5941 gccggctcga tatccaccgc aatcccccgc cacttcccct gccggaaacg aagcacgctg
     6001 agcgtggccc gcgtggcgtg gccgatcacg atcgcggtcc agatgtgaac cgcctcgagg
     6061 ccgtacagag actcgagcat ggcgcacagg ccgagcggga cgatcacctg cgacacgatc
     6121 gagatgtaga gcgggctgcg cgtgtcgccc gtcccctgga ggccgccggt atagctgagc
     6181 gccacggtga tgaagaagcc tgacacggtc aggaacccga gcagcgtccg cccgagtgac
     6241 agcacgagcg ggtcggtcat gccgaagatt gccagcagat agcccggtat gagcaggaac
     6301 atcgcgccga cgaccgcggc gacgcccagc ccgtacctcg acgcgacgcg cacgccctcc
     6361 atcgcgcgct cggcgttgcc ggcgccgagg ttctgcccgg cgatggtcgc cgaggccccc
     6421 atcagcccga ccgatgtcca ggtgatgagc gagaacagct ccgtgtagcc aaccgcgtag
     6481 gccgcctgtg ccgcggcgct gtgctcgagc gagccgatga agcgcagcag cagcacgccg
     6541 ccgatgttca tcgcgattcc ctgcacgccg gtcggcaggc cgaagcggaa gagcgatcgc
     6601 acgatcccga agtcgggccg caggtccatg ccgcgctcga agtggatgac cgacttcggc
     6661 ctggtcagct gccacagccc gtacacggac acgctggtgc tgctggcaat ggttccaaac
     6721 gcggcgccga ccgtgccgaa gatcggaatc agcacgacgt tgaacacgat cgtcagcact
     6781 gtcatgacga cgccgaggcg caggggcgtc tgcggatcgc cggcggcgcg gaaagcgccg
     6841 ctcagcatga agaacatcat catgccgaag atgccgacga acatcgcgcg caggaacggc
     6901 agtgcttccg cctgcacgtc cggcgcggcg ttgacgagcc cgagcagcga cggcgcggcc
     6961 aggtagccca ccgccgcgag gacgaccgac agcgcgaagg cggtcaggaa cgcctggtag
     7021 acgacgcggt tgaccttgcc gggctcgttc gccccggcaa agcgcgccac gagcacggcc
     7081 atgccggtga acagcgagct gatgaagacg atgacgacga ggatgatctg ccagctgacg
     7141 ccgatggccg cgttggcggt gtagccgacg aagtggccga ccatcgcgtg atcgatgatg
     7201 ccctgcagcc cggcgatgac attctgcagc atcgtcggcc aggccagctt ccagaccgcc
     7261 ggtccgattg gaccttcgac gatggaccgg tcgaattttc tctgcgctcc ccggggctcg
     7321 gccacagaag aggaagtgta ctacctgctc gcggaaagca gtaagatggc ggccagcgag
     7381 gttcggatat gacgcatcga cgctgtgctt ccgtgtcgct tgccatcgcc gtcgccatcg
     7441 tgtgggtctg gtccgcgaac gccgtcgcgc aagcgcccgc cctctcgccc cgcgccgcca
     7501 gcgccgcgcc cgcctggagc cacggccgga cgccggacgg ccagcccgac ctgcagggga
     7561 tctggatcaa cttcgacagc acgccgttcg agacgcccgc ggtccagccc gcggcccagc
     7621 ccgcgccggc cgccggcggc gccgcggctg ccgcgcgcgt gggccccgcc tcggagttcg
     7681 ccgatcacaa ccacaagatc agcgcgcgac gagcgtcgat ggtcatcgat ccgccggatg
     7741 gccgcgtgcc ggtgatgaag tgggcggagg acagacgcga ctacgatctg gcgcacattc
     7801 ccgacgcgcc cgagcacgag acgccgtggg tgcgctgcat cacgcgcggg tacccggccg
     7861 gaatgtttcc cgcggggtac aacaacgcct atcagatcat tcagatcccc ggctacgtcg
     7921 tcatggtgtt cgagatgatc cacgagacgc gcatcatccc gatcgacggc cggccgcgcc
     7981 tcggcgagaa gatccggcag tggaacggcg agccgcgcgg acgctgggaa ggcaacacgc
     8041 tcgtcgtcga gacgaccaac tacaacaaca aaggctctat tgccacgagc gcggccaccg
     8101 gccgcatgcg cggcatcccg cagaccgagg cgatgcgcgt cgtcgagcgc ttcacgcgaa
     8161 tcgacgcaaa cacgatcaac tactcggtga ccatcgacga ccccaaggtc tacaccaggc
     8221 cgtggaccgt ctcgctcccg ctcaatcggg acgacagcta cgagatgttc gagtactcct
     8281 gccacgaggg gaactacgcc atggccaacg ccctcagctt cgggcgcgtg cgcgacaagg
     8341 aagccgggca atagacgtcg aggcactcga atcgagcagg agatcgtgtg ttcacgcttc
     8401 tcagttctcg cttctcggtt cgtgttcacc ttcgttcgtc agttcggtgt ctcctgcggc
     8461 tttcgattgg gacgtgcgcc atcgccggga cggcactcgt ggtcacgagc tgtcgtcagt
     8521 gggatcattt tcgcccgcgc actcacacgg tcgcaatgtc gcccgacggc acgatcaccg
     8581 cgtgggtgcg acaggacttc aacatcgatc ctccagacga ccacatctat atccggcgcc
     8641 agggccaccc cgtacgtgtg atcaaggatc tcccgccgga cggggaatgg tgtcgcgtga
     8701 tcgtatggtc gcccgacagc acgaaagtgg gcttcgttat caatgacgcg cggctcgata
     8761 tcttcgatgt cacgcacgat cggtggctgg cgcccgtggc gctcgtgttc gacggctaca
     8821 acgaagcgcg gcgagtgcag ttcagccccg acgggcgcgc gatcctgtac gacgtcgtcc
     8881 agcgcgctgt cgtccgactg acgtaccgtg acggccgcgt catcgaggcg ccgctctccc
     8941 ggttgagcga caaatcaagg tcttccatcc gcgaggaagt agtccttggt gccgctcggg
     9001 ttgagctttg acgacagcaa tcccgtgagt cgaagcttgc gggtgcgaac gtggtacacc
     9061 gaacgcggat gaagtccgtc acccgcctat aatgccttcg tttccatttc ggactgacga
     9121 ttattgtcgc ctttcaaccg tttgcacccg tgacgtcgcc acggaggcac ggagacagaa
     9181 atcgaaactc cgtgtctccg cgtctccgtg gttcgtggag atcggtgatt ggtgaaacat
     9241 gcgaatcaga gagctggcgc tgatagggtt cgtgagcgta gtgagcggct ggacggtcgc
     9301 cgcccagcgg gggccggctc ccgcgccggc cgggcaggag gcgatcgtcc cgttcaggat
     9361 ccaggtgccc gatgccgtgc tgtccgatct caggcagcgg ctggcgcgca cgcgcttccc
     9421 cggcgagatt gccgggtccg agtgggacta cggcaccaac ctgggatacc tgaaggagct
     9481 cgtcgcctac tggcgcgaga agttcgactg gcgggcggcc gagcgccgcc tcaatcagtt
     9541 cgaccagttc acgacgaaga tcgacgggct cgacattcac ttcatccacc agcgctcgaa
     9601 ggatccgaag gcgctgccgc ttgcggtgac ccacgggtgg cccggctcgg tcgccgagtt
     9661 cacgaagatc atcggtccgc tgacgaaccc ggccgcgcac ggcggcagcg cggccgatgc
     9721 gtttcacgtc gtcgcgatct cgcttcctgg cttcggcttc tcgggcaagc cggccgaacg
     9781 gggttacggc cccgagcgca tcgccggcat catcgcgaag ctgatggcgc ggcttgggta
     9841 cacgcggtac gggttgcagg gcggcgactg gggcagcgcg atcagccggt tcgccgcgct
     9901 gaacgacgcg agccacgtgg cgggactgca cgtcaacttc tgcctggcgg ggccgccgcc
     9961 cggcgtcaag gatcccaacg agggcgtgac gcccgttgag ctcgagcgct cgcgcgcgcg
    10021 gcaggcgttc ttcgagaccg agcgcggcta cttcctggag cagagcacca agccgcagac
    10081 catcggctcc gcgctggacg actcgcccgc ggggctggct gcgtggattg tcgagaagtt
    10141 ccggtcgtgg agcgactcgg acgggaacgt ggagaagtcc ttcacgaagg acgacctgct
    10201 gaccaacatc acgatctact gggtcacgca gacggccacc tcgtcagcgc ggatctacta
    10261 tgagaaccag cgcgccaagc cggcgcaggg gcgggtgcag gtgccgacgg cgtgcgccgt
    10321 gtttcccaag gagatcagca tcccgccccg gcgctgggtc gaggcgcagt acaacgtgac
    10381 gcgctggacg gagatgccgc gcggcgggca tttcgccgcg ctcgaggagc ccgagcttct
    10441 cgtcgaggac atcagggcgt ttttcaggat gttacggtga cggtgcgaac cggagccggt
    10501 tcgcatgcga tatcagctgc ttgagtggga ctcggacttc ttcggaatcg gggtggctcg
    10561 aatcatcccg gctcggttga gcagggagga gttgacggag acgctcacgc agttacagca
    10621 gcagcgcgtg acactggcct attggttcgc cgatcaggaa ttcgaccatg acgtgattac
    10681 ctcgctcggc ggcgtctttg tggacagaaa gacgacgttt gtcgtggatc tccgcacctt
    10741 gcatttcgac aggattcctc ttgccgatcg tgtcgagcca tttggggatc cgatgcctgt
    10801 agcggatctc gagagcctgg ccgttcaaag cggcgaattc tcgcggttcg ccgttgaccc
    10861 caacctgcca aaagacaagt tcgtcgccct gtacaaagtg tggatagatc ggtccattcg
    10921 gaaggacctc gcctccgagg tcctggtcat tcgagatcgc gacacgattg cgggtatggt
    10981 gacgcttggc gaaaagaacg cgcgaggcgc catcggcctc attgccgtcg acagtcgtta
    11041 ccggggcaga aagtatggtg aggcgcttgt ccgtgccgcg cagagatggt tcgcgacgca
    11101 cggatacgag tacggacagg tcgtcacgca ggggcaaaac gtcgccgcgt gctatctgta
    11161 cgaaaagtgc ggttactcgg tcgaacgggt ggaatgtgtc tatcatttct ggattccatg
    11221 actgtatgcg tgcgagcctg tgcgagcgtg atcctcgtcg gtgcggcagc gcttgccgtc
    11281 agtactcagg gattccatgc gttccagggc ccgccgtcca ttgcgcggcc gggtgccacc
    11341 cggatgtcgt ccaacagggt ttcgtccacc gtcgccgcga gctacgtggc tcgagagggg
    11401 cgactggtcc tgctcgtgtt gtggcgcgga agtccggcat ggctcgagcg cggcggtgga
    11461 gattcgagcc gcggaggcgg cgcgagcggg cggacgtggc tgcaggacgc gatcggcggg
    11521 gtgacgctcg acgtcgagat cgatgatgca actcgggtcg ccacggtgct cggacaccgg
    11581 gtgcccctcg aggacgggaa cgtcgtcctg gtcgatcgcg tggacaccga acccgcggtg
    11641 ctgcgggtgt tgcacgtcga cccagccgtg ccgctcactc ccgatgcgat gtacgtcgtc
    11701 atgaaacggg aacccgagct cgtggagttt ctgcagtgtg atctgtttcg g
//
LOCUS       DBNGSH010000437        18756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_889408, whole genome shotgun sequence.
ACCESSION   DBNGSH010000437 DBNGSH010000000
VERSION     DBNGSH010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 18756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18756
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_889408"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="AC16CY_09910"
     CDS             <1..560
                     /locus_tag="AC16CY_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3354146.1"
                     /translation="VLVGSIEPGSAASRAGLQRRDVITTLNGEKLADSNALRNRIAAA
                     KPGSTVTLGIIRDGRNQTLSATLSEREAAQNRASAAEERSSGNREYGAFGMTVEPLTP
                     DLARELGLPSRKEGVVVRSVNPDGAAADAGLQPGDVVTEVNGQAVTTPSELKSALSAS
                     GDRPALLLITRDNAEFYRPLRRPRS"
     gene            624..1097
                     /locus_tag="AC16CY_09915"
     CDS             624..1097
                     /locus_tag="AC16CY_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354147.1"
                     /translation="MLIKVTIWAALLAYTAGEWGRVRTPRASWARVVWTVGALLYAAH
                     VAAAFTFAYRWSHAAAYAHTARQTADLAGLDWGGGIWVNYAFTVVWLAEAIWWWAAPS
                     RYLSRPRPLDLIVRAVFLFMIVNGAVVFVAGPAKWMGVAIVVALCAIWRTPVRRV"
     gene            complement(1138..1986)
                     /locus_tag="AC16CY_09920"
     CDS             complement(1138..1986)
                     /locus_tag="AC16CY_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HIR3354148.1"
                     /translation="MNDRSHGEAPLACSVRRCGRPLARRDRAFICQAGHSFDIARSGY
                     VNLLQPQDRRSPSAGDSKAVVEARVELLAAGVGKTLIDEVARRVASLGLARGAVAVDL
                     GSGSGDALAAIGSRLEVTGVGIDVSAAAVEHAARCFPTLTWVAANADRRLPLLDRSVD
                     VAISIHGRRNPREAARVLGTGGCLIVVLPAPDDLIELRAIVQGEGSIRERTERMLAEH
                     DPFFAPAERASIRETLEVDRRSLLNLLRITYRGARASAAERVKSLQAMRITFSSDLFV
                     LRLRRV"
     gene            complement(2157..3683)
                     /locus_tag="AC16CY_09925"
     CDS             complement(2157..3683)
                     /locus_tag="AC16CY_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR3354149.1"
                     /translation="MLKRSIVVIHRWLGVALCLLFLLWFPSGIGMMYFPMPSVSPADR
                     LERAPSLDASTVVLSPAEAASKLGVGPGALRLSSFDGRPAYRTGGRDGGAVLYADTGE
                     EQGDVSMDMVRRLASAWTGKPANDAAVHEVTEVDQWTLQSRLGAIAPVWKFVWPGGEH
                     VYVSQSLGEVVQYTTTASRIGAYLGPIPHWFYFTPLRKHGPEWSRVVIWSSAVGTVSA
                     ILGLVIGVWMYSPSRRYRYDGAPTSVPYRGQKRWHMVLGLVFGVATATYAFSGMLSMD
                     PFPSLTERPRARGRDGEELNIARVLRGRVDPGAYDAKHPRQVLEQLGELKVKEIELIS
                     FAGEPFYLARLDDQSTRIVPLTGDPARAFETTRIIDVVTAAGKGRVTTRVLEQYDRYY
                     LDRERLLPLPVVLAQATTDDETRYYIDPNTARVVRTYDSSNWVGRWLYNGLHSLNFPW
                     LYNYRPLWDIVVITLMLGGTALCVTSLVLAWRVLGRKLARAVRTRSAAEEMGLSEDLA
                     "
     gene            complement(3779..4624)
                     /locus_tag="AC16CY_09930"
     CDS             complement(3779..4624)
                     /locus_tag="AC16CY_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354150.1"
                     /translation="MNAKRAMTALAVVACTLAAPAPSAAQSVPASEREALVRLIVDRG
                     GRAEDADALVDQANAAGAKGLPVTPLTNKIREGIAKGHEPARIEGLIRQLTGHLESAD
                     RLIRDLPQPPGAAGREASVTLLADAFFSGVTSDEVNEIRRLSQAPGGAPMSPDGLAGA
                     AKGLSFIKEARLSAAEGAAVMSEAARGGFRSLDMVDLGREIKRRERDFREGRATLRAL
                     REAIARGSRPEQLLRERRARNADRPATPRRDPAARPEPAARPERPRPPDRPDRPERPE
                     ATRAR"
     gene            complement(4614..4982)
                     /locus_tag="AC16CY_09935"
     CDS             complement(4614..4982)
                     /locus_tag="AC16CY_09935"
                     /inference="COORDINATES: protein motif:HMM:NF027875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HIR3354151.1"
                     /translation="MRLRLSIVLMAAACASGSCATATGPMPPVATPAGVRFVFEDDEA
                     RSVALVGSFNHWSISSHPMSRAGSSGPWTAVVALPPGEHLFMYVVDGARWISPPLAED
                     YADDGFGARNGIVVVRSNER"
     gene            complement(5124..5816)
                     /locus_tag="AC16CY_09940"
     CDS             complement(5124..5816)
                     /locus_tag="AC16CY_09940"
                     /inference="COORDINATES: protein motif:HMM:NF027875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HIR3354152.1"
                     /translation="MREYDPSSGSELLVQRFIDQELSGEERIELLVQLGRDQALRERL
                     IELERLMLGVADLPRPAATHEFVARVMERTAERPSAWQRAIDALWAPHAFRWNLAGAT
                     ALAALLVAGALSARSLMVPPASTPAPVAAAPAGPSPVLVRLVVLQPDAMSVALAGDFN
                     GWDPMRTPLEQTSSGAWTVTIPLEPGRYEYMFVVDGKEWIADPFAVEQSDDGFGSRNA
                     VLDVRPPMEASL"
     gene            complement(5813..6481)
                     /locus_tag="AC16CY_09945"
     CDS             complement(5813..6481)
                     /locus_tag="AC16CY_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3354153.1"
                     /translation="MAQDEAADDASLVRASQRGDRDAFGILVSRHASAVLSVTTRMLG
                     STADAEDVAQEAFVAAYKALQGFQLESKFSTWLYRIAVNKCTDVLRTRRPGTVSIDAG
                     AEEGAAWEPADEDTPDRALEQGELAGQLDQAVQALPPLYRESFVLRHVEGLGYDEMST
                     ILGVHRDTLKMRVYKARHILCRTLAHLVDRANDSPSTEISRADQREVRSRSRKDTPVD
                     GARR"
     gene            complement(6488..7384)
                     /locus_tag="AC16CY_09950"
     CDS             complement(6488..7384)
                     /locus_tag="AC16CY_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transporter family protein"
                     /protein_id="HIR3354154.1"
                     /translation="MKTSGCILTAVLLLLLVPASSRAQDEQWQVGSTPSFTSGTYGTS
                     SRTDVFYTPFTARRLFTDGDLTLVFPIMCLRGTGAVTIVDGTPMPIDTSRTGATSSGT
                     TTRTGTTTSDPATRTGAAGATTTAAAPDTATPPGTTCGAGDIGVRGRYYVLDERSWWP
                     TIAVRAHLKVPTAAPGKGLGTGRPDEGVGVEISRMVGGGFVTMLDAGYTIIGKPTGVE
                     YRNGWWYDVGVGQDLADGAVNVSVFFEEYSTIVPGLENARDILAAVTVKGALWRLQVS
                     GGFGLSDGAPDHGFMLGASRRF"
     gene            complement(7381..7635)
                     /locus_tag="AC16CY_09955"
     CDS             complement(7381..7635)
                     /locus_tag="AC16CY_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354155.1"
                     /translation="MDATGIGIAIAGGSIGARGAMTGESIAASVATNGAPIGRHDLIG
                     RSGASARPGRSVRNGRNVLPDPIGRNESSARRNRIEGDDP"
     gene            complement(8083..9117)
                     /locus_tag="AC16CY_09960"
     CDS             complement(8083..9117)
                     /locus_tag="AC16CY_09960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3354156.1"
                     /translation="MVADVAVAGGGPAGAALAIELGRRGVRVNLYEKAQHPRLKACGE
                     GLLPHGVAALAGIAGLPDFPRIRGLRFVAGGASVEADFPDAFGLIVRRDRFDAWLFER
                     AAATPNVDARMKTPYRDGGERFTVGADGRHSVFHKRLSAHRPARRRVGLSSHAGGLDG
                     LGDRAEVFYHRDGEVYVAPTGGGEALVAALFHQERFRRDGLTHLLKAIPELRERTSRI
                     EWTTPVLAAAPLGLRVPRVVDHNLLLIGDAAGAPDPITGDGIALALASVRPAADAIVS
                     GDLLSYERTRLAMGRTADRLGRVLLAISRAEGRAARALLRRPSLVPTFMDVALGRREL
                     NVSTVLRAFL"
     gene            complement(9111..10160)
                     /locus_tag="AC16CY_09965"
     CDS             complement(9111..10160)
                     /locus_tag="AC16CY_09965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012421.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HIR3354157.1"
                     /translation="MPHIQALATAVPPHRLDQSFARREMARICEGQPHLERLLPVFDH
                     AGVETRHLVRPPEWYLAPRSFEERNDEYAARALELAERAARQCLASSGTAPDQIDHIY
                     FVTTTGLATPSLDALLAGCLGLRVDVRRSPLFGLGCAGGAGALVRACDVLTAKPRERA
                     LVVSVELGSMIFSPRAQTPTDLVGVALFGDGAAAALVGGDDTAPDGIRVVGTRSRLFP
                     DARHLMGWRFTSDGMRLILSRDIPSLVRERVAPLVDEFLGNAGMTVKEITHHVLHPGG
                     PKVMATYRSAFGLPDDALAVARDCMRRYGNLSSAAVLVMLSNLVASGKPRAGDTGLVL
                     ALGPGFATELLALSW"
     gene            10394..10915
                     /locus_tag="AC16CY_09970"
     CDS             10394..10915
                     /locus_tag="AC16CY_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3354158.1"
                     /translation="MDNSRRLIVSFGPSQDPPSTSSRRQFCAHACQAVSLVAVGALIP
                     ACGGEGDPNGPDPVPDGNSPLPLLSGTATGNTVSVPTDGALAAVGSAALVSSPVGSFL
                     VARTAQTTFSVLTAVCTHEGCTVDLFNGALFVCPCHNSKYTTAGAVANGPATQALRSF
                     PSTLTGTTLTFTV"
     gene            complement(11129..12430)
                     /locus_tag="AC16CY_09975"
     CDS             complement(11129..12430)
                     /locus_tag="AC16CY_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3354159.1"
                     /translation="MTTAPASAARNNPVEARRVALAGGVGTTIEWYDFFIYGTAAAVV
                     FAPQFFPQASDLAGRLAAFATFAIGFIARPLGGIAMGHYGDRLGRKSMLVWSLMLMGL
                     ATLGIGLLPNYERIGVWAPALLVILRFVQGFALGGEWGGAVLMSVEHAPGGRRGFFGS
                     FVALGLPVGIILSNLVFLVISGLFEPDEFAAWAWRIPFLASAALVVVGLWVRLGLSES
                     PVFAEVLRTASARRMPILDVLRNDWRAVLIAAGSYVGISANGYVVIVYFVSYATRELG
                     LPLPTTLTLLLTGAVLFAGSIVVFARWSDRIGRRRITQWGLGALVLWSLVFFPLADTK
                     SIPLIALALCGMFIVQGAYIGPQPAVFSELFPAAIRYSGASLSLTLGTILGGALAPVI
                     ATTLYNAAGNSRLVTAYITVMSLLSFVSSLWLKETYRRELR"
     gene            12481..13719
                     /locus_tag="AC16CY_09980"
     CDS             12481..13719
                     /locus_tag="AC16CY_09980"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3354160.1"
                     /translation="MSSTPSPQRRLWFVLVIATLVAVGVHTGAGQQTVPFQGGIPVAP
                     SGLAGRKLPNLPMEFPTAEGHRIRVVAVTRALEFPWSLATLPDGTMLVTERSARLRII
                     RNGVLDPQPVPGGPTGYFTGESGLPGAVHGYMDVVLHPQFAQNRLVYLSYTKPIDEKR
                     RTVGIARGRFDGRALTDVKDIFVLDQAGTSRLAFGRDGMLYVTTTGQDPQNANTQGGK
                     TLRLRDDGTIPKDNPFAGRANHKPEIFTLGHRNSLGLAMHPGTGEMWQNENGPNGGDE
                     INILKPGANYGWPVVSYGRTYPGPWQSERPGHVGFEPPIVYWMPAIAVSGMAFYTGDR
                     FPKWKGDVFVGGLRMGEIPGTGHIERILFNEKMEELRRESLLVELHQRIRDVRQGPDG
                     LLYALTDEKEGTLLRIEPVN"
     gene            13745..14182
                     /locus_tag="AC16CY_09985"
     CDS             13745..14182
                     /locus_tag="AC16CY_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3354161.1"
                     /translation="MRSKPIDGWRWRIHACAFLVAGLAWAAHAQQTPANPDTSSNFTG
                     GKVTTLMGEGRISYYVFESGARTRWHSHEGGQLLLAEEGMGRTQVRGGAVRDLRPGDT
                     AWSPPGITHWHGASPGHSAKMYQVSRGMTTWMEAVSEGDYAGK"
     gene            complement(14242..15123)
                     /locus_tag="AC16CY_09990"
     CDS             complement(14242..15123)
                     /locus_tag="AC16CY_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3354162.1"
                     /translation="MIRHAAAIVVALCLFTAPVAAQNTEITINAASASVHKSPSAGSP
                     VIGRANRGTTLEVTREVGDWVKVTWPSAADGSGYVRTNAGSIGKAAPVAARAASSASR
                     PAAQPASAPAPRLTTSANAEPQRVGTVPPRPQSSSNISTPRHQIGLGARLGGSALGLG
                     ASARAWSRGPLGVQFDVSRFSQTDPTSTVMTSTQFGPSALYTFGDRISDYIWLRPYAG
                     AGATFNRSTMTPFGGLPVSDSNAGFQAFGGGEFSVASLPQLAVSADMGYHWFESPFAG
                     HELGGFSVSVAAHWYIK"
     gene            15295..16113
                     /locus_tag="AC16CY_09995"
     CDS             15295..16113
                     /locus_tag="AC16CY_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3354163.1"
                     /translation="MIEARNLTKQYGRVTAVDGLSLTVAEGELVVLLGGSGSGKTTTL
                     KMLNRLIEPTSGRVVIDGQDALSVAPHELRRRVGYVFQRVGLFPHMNVGENVAITLTL
                     MGWDRGRAAARVEELLELVELDPAAMRERRPAELSGGEQQRVGVARALAASPRLMLLD
                     EPFGALDPLTRDRLQQSFLRIRRTLRLTAIFVTHDMVEALLVGDRIGVMHHGRLVQVG
                     TPSQLLQAPADDYVRQLMNTPRRHAALVDSLLARPSTVAQGVPSSVEGRDSESS"
     gene            16110..17630
                     /locus_tag="AC16CY_10000"
     CDS             16110..17630
                     /locus_tag="AC16CY_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3354164.1"
                     /translation="MKEQLALLPQYVTAHLQLTLFALAAGMAFSIPAGIAITRSPRFE
                     RPVLAVASVIQTVPSLALLAIMVPALSALGLRSIGFLPALIGLSLYSVLPILRNTVTG
                     LSGLDPALTEAARGVGMTPRQQLWRVELPLAMPVILAGVRTATVWTVGMATLSTPVGA
                     ASLGNYIFSGLQTRNLTSVLVGCVGAAALALVLDGLVRALGAALVGRRRSPLFVVLAT
                     LGVLYAYSSVTFVQGLRRSGPDRIVVGAKTFTEQYILGRILALQIEKSTGSPVEVVSS
                     LGSTVVFDALRAGEIDAYVEYSGTIWATIMHRDSAPTTRAALLSEVSRFLESQHGITV
                     AGPLGFENAYALAMRRGQATGLAVKSISDLARHAPRLAIGGDYEFFDRAEWRAIRDIY
                     GLEFRDQRSMDPSLMYQAVANGEVDVIGAFSSDGRIAALDLTVLQDERGAIPPYDAII
                     LAGPRLVRERRDVLAALSALAGRIDADEMRRMNLAVDQEGAAPDDVAARFVRAESP"
     gene            17678..18700
                     /locus_tag="AC16CY_10005"
     CDS             17678..18700
                     /locus_tag="AC16CY_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3354165.1"
                     /translation="MSDPILHVKPLGHRWQTEDPFLFCVHHIDLYPKGNEQFGPAAPL
                     EGRNIGQDFDGLDGWRMYHGDVVPGFPQHPHRGFETVTIVRSGFIDHSDSLGAAARFG
                     SGDVQWLTAGAGVVHSEMFPLLDREGPNTLELFQIWLNLPSKDKLVDPYFTMFWDRDV
                     PRRRFTDAAGGEAEVTIIAGTLDGTTPPVPPPHSWASRPESDVAIWSIVLEKGASWTA
                     PPAKTGSTLRTLNFFAGKSLRAGDRVVSGLSAIQVRADQPVGIEAVDGRAEILLLQGR
                     PIGEPVAQYGPFVMNTRDELQRAFADYQRTQFGGWPWKESAPVHGGRDAGRFARHADG
                     RVERVG"
BASE COUNT         3087 a         6414 c         6288 g         2967 t
ORIGIN      
        1 gcgtgctcgt cggaagcatc gagcccggaa gtgcggcgtc gcgtgccgga ctccagcggc
       61 gcgacgtcat cacgacgctc aacggtgaaa agctcgccga cagcaacgcc ctgcgcaatc
      121 gcatcgcggc ggccaagccc ggatcgactg tgacgctcgg catcatccgt gacgggcgca
      181 accagacgct cagcgccacg ctcagcgagc gcgaggcggc gcagaaccgt gcatcggccg
      241 ccgaggagcg cagctccggc aatcgggagt acggcgcctt cggcatgacg gtcgaaccgc
      301 tgacgccgga cctcgcgcgc gagctcggct tgccgagccg caaggaaggc gtcgtcgtgc
      361 gatcggtgaa ccccgatggc gccgccgcgg acgcgggtct ccagccgggc gacgtcgtta
      421 cggaagtcaa cggccaggcg gtcacgacgc ccagtgagtt gaagtccgcg ttgagcgcgt
      481 cgggcgatag accggcgctc ctgctcatca cgcgggacaa cgctgagttc tacaggcctc
      541 tccggcgccc ccgatcttag acatccggtc tccagtcttc ggtctcccgt cctgagcagg
      601 accggagacc gtagactgaa accgtgctga tcaaggtcac gatctgggcc gcgctcctcg
      661 cttacacggc gggcgaatgg ggccgggtcc ggacgcctcg tgcctcctgg gcccgtgtcg
      721 tctggaccgt gggagcgctg ctttacgccg cccacgtcgc agccgccttc acgttcgcgt
      781 accggtggag tcacgctgcg gcgtacgcac acacggccag gcagaccgcg gatctcgccg
      841 gactcgactg gggcggaggc atctgggtca actacgcctt cacggtcgtg tggctggcgg
      901 aagcaatctg gtggtgggca gcaccgtctc gatacctgag ccgcccgcgt ccactggacc
      961 tcatcgtgcg agcggtattc ctgttcatga tcgtcaacgg cgccgtcgtc ttcgtggcgg
     1021 gtcccgcgaa gtggatgggc gtggcgatcg tggttgccct gtgcgcaatc tggcggaccc
     1081 ccgttcggcg agtctaaaga ctcgccgcta ctgcaggact ccggtagagg cgggtcttta
     1141 gacccgcctg aggcgcagca cgaagaggtc ggagctgaag gtgattctca tcgcttgcag
     1201 cgacttcaca cgctcggctg cgctggcgcg cgcgccgcga taggtgattc gcagcaggtt
     1261 gagcagcgag cgtcgatcga cctccagcgt ctcgcgaatc gacgcgcgtt cggccggggc
     1321 gaagaacggg tcgtgctcgg cgagcatccg ctcggtccgc tcacgaatcg agccttcccc
     1381 ctggacgatc gctcgcaact cgatgaggtc gtccggcgcc ggaagcacga cgatcaggca
     1441 gccgccggtg ccgagcacgc gggcggcttc gcgtggattc cgccggccgt ggatcgagat
     1501 cgccacgtca accgagcgat ccagtagagg cagcctgcgg tccgcatttg ccgcgaccca
     1561 cgtgagggtg ggaaagcagc gcgccgcgtg ctcgacagcg gcggccgaca cgtcgatgcc
     1621 gacgcccgtg acctcgagcc ggctcccgat ggcggcgagt gcgtcgccgg agcctgagcc
     1681 gaggtcgacc gcgactgcgc cgcgggcgag gccgagggag gcgaccctcc gggcgacctc
     1741 gtcgatcagg gtcttaccta cgccggcggc caggagctcg acgcgagcct cgacgacggc
     1801 cttcgaatcg cctgccgagg gcgatcgccg gtcctgaggc tgaagcaggt tgacgtatcc
     1861 gctgcgcgcg atgtcgaagg agtgaccggc ctggcagatg aacgcgcgat cgcggcgggc
     1921 gagcggacgc ccgcagcgcc gtacgctgca cgcaagcggg gcctctccat gagatctgtc
     1981 gttcacactt ctctcttctc gggttctcgg ttcgtgttca attgcgaggt tcgtgttcga
     2041 ggttcagggt tgactgttcc ccgcgcgata ctcgaacccg gaacagcgca cctgaacgcg
     2101 aacgtgaaac tgaacacgaa cggagaagtg agaaccgaga agagtgaaca cgacgatcag
     2161 gccaggtcct cggacaggcc catctcctcc gcggcgcttc gggtgcgcac ggctcgcgcg
     2221 agcttgcgtc cgagcacccg ccatgccagg acgagcgacg tgacgcagag cgccgtaccg
     2281 cccagcatca gcgtgatgac gacgatgtcc cacagcgggc ggtagttgta cagccacgga
     2341 aagttcagcg agtggaggcc gttgtacagc catcggccga cccagttgct cgagtcatac
     2401 gtacgaacga cccgggccgt gttgggatcg atgtagtagc gcgtctcgtc gtcggtcgtc
     2461 gcctgcgcca ggaccacggg gagcggcagc aaccgttcgc ggtccaggta gtacctgtcg
     2521 tactgctcga gaacgcgggt cgtcacgcgg cccttgccgg ccgccgtcac cacgtcgatg
     2581 atgcgcgtgg tctcgaaggc gcgggccgga tcgccggtga ggggcacgat ccgcgttgac
     2641 tggtcatcca acctggccag gtagaacggc tcgccggcaa acgagatcaa ctcgatctcc
     2701 ttgaccttca gctcgccaag ctgctcgagc acctgacgcg gatgcttcgc gtcgtacgcc
     2761 cccgggtcta cccggccgcg caagacccgg gcgatgttca gctcctcgcc gtcccgcccc
     2821 cgcgccctcg gccgttccgt caacgagggg aacggatcca tcgacagcat cccgctgaac
     2881 gcgtaggttg ccgtcgcgac gccgaacacg aggccgagca ccatgtgcca gcgcttctgc
     2941 cctcgatagg gcacgctcgt cggagcaccg tcgtaccggt atcgcctcga gggcgaatac
     3001 atccagacgc cgatcaccag gccaaggatc gccgagaccg tgcccacggc cgacgaccag
     3061 atgaccaccc ggctccactc cggcccgtgc ttgcgcagcg gcgtgaaata gaaccagtga
     3121 ggaatcggtc caaggtacgc accgatgcgg gaggcggtgg tcgtgtactg caccacctcg
     3181 cccaggctct gagacacgta cacgtgctcg ccgcccggcc acacgaactt ccacacggga
     3241 gcgatcgctc cgagccggga ttgaagcgtc cactgatcga cctcggtgac ctcgtgcacg
     3301 gccgcgtcgt ttgccggctt gcccgtccac gccgaggcaa gccgcctgac catgtccatc
     3361 gacacgtcgc cttgctcctc gccggtgtcc gcgtagagga cggcaccgcc gtcgcggccg
     3421 ccggttcgat acgcggggcg tccgtcgaag gacgagaggc gcaaggcgcc agggccgacg
     3481 ccgagcttgc tggccgcctc cgcgggcgac aggacgactg tcgaggcgtc gagcgacgga
     3541 gcccgctcga ggcgatccgc cggactgacg ctgggcatcg ggaagtacat catcccgatg
     3601 cccgacggga accacaggag gaacaggagg cacagcgcca cgccgagcca gcggtggatc
     3661 acgacgatcg atcgcttgag catttctctg ctgcggatgc taccccgcgc aagggcccgg
     3721 gtgctctgaa aagatcctga attcttcgtc aggaaacgcg cagcgccgcg cggcgcgctc
     3781 agcgtgcgcg cgtcgcttcg gggcgttcgg gtcgatccgg ccgatcgggg ggacgaggcc
     3841 gctcgggccg cgcggccggc tccggccgag cggccggatc ccgtcgcggc gttgcgggac
     3901 ggtccgcgtt ccttgcgcgc ctctcgcgga gcaactgctc gggacggctt ccgcgcgcga
     3961 tcgcttcgcg caacgcccgg agtgtggcgc gtccctcacg gaagtcgcgc tcgcggcgct
     4021 tgatctcgcg cccaaggtcc accatgtcga gcgatcggaa accgcctctg gccgcttcgc
     4081 tcatcaccgc cgcgccttcg gccgcggaga gcctggcctc cttgatgaac gacagcccct
     4141 tggccgcgcc ggcaaggcca tccggcgaca tcggcgcgcc gccgggcgcc tgggacagac
     4201 gccggatctc gttcacctcg tcgctcgtca cgccgctgaa gaacgcgtcg gcgagcagcg
     4261 tcaccgacgc ctcgcggccc gccgcgcccg gcggctgcgg aagatcacgg atcaggcgat
     4321 cggccgattc gagatggccg gtcagctgtc gaatcagccc ctcgatgcgc gccggctcat
     4381 gccccttggc gatcccctct cgaatcttgt tggtgagcgg cgtgacgggc agtcctttcg
     4441 ctccggccgc gttggcctga tcgacgagcg cgtccgcatc ttccgccctg ccgcctcgat
     4501 ccacgatgag gcgcaccaac gcctcgcgct cggatgccgg caccgactgg gcggcgctgg
     4561 gcgcgggcgc ggcaagagta cacgcgacga cggccagcgc cgtcatcgcc cgcttagcgt
     4621 tcattcgatc gaaccaccac gattccattt cttgccccaa agccatcgtc ggcgtagtcc
     4681 tcggccagcg ggggactgat ccaccgcgcg ccgtccacga cgtacatgaa gagatgctcg
     4741 ccgggcggca gcgccacgac cgcggtccac ggcccggatg atcccgctct ggacatcgga
     4801 tgcgacgaga tcgaccagtg attgaagctg cccacgagcg ccacgctgcg agcctcgtcg
     4861 tcctcgaaga cgaatcgcac ccccgcaggc gtcgcgacag gcggcatcgg ccccgtggcc
     4921 gtcgcgcacg acccgctcgc gcacgccgcg gccatcagca cgatcgacaa ccgcagacgc
     4981 atccgcgttg gagactaacg catttttcac tctgctgcgc cccaagtccg gctgccgcct
     5041 tcgcgcgtga agcgcgctac ggcgagcctc gctgaagcct tcggcggaag cgggaagccg
     5101 gacactacgt ccaacgtccg cgatcacagc gaggcctcca tcggcggccg cacgtcgagc
     5161 accgcgttcc gcgagccgaa cccgtcgtcg ctctgctcga cggcgaacgg gtcggcgatc
     5221 cactccttgc cgtccacgac gaacatgtac tcgtagcggc cgggctcgag cgggatggtc
     5281 accgtccacg cgccgctcga cgtctgttcg agcggcgtcc gcatcggatc ccatccgttg
     5341 aagtcgccag ccagcgccac cgacatggcg tcgggctgga gcacgacgag cctcacgagc
     5401 accggcgatg gcccggccgg cgccgcggcg accggcgccg gagtcgaggc aggcggcacc
     5461 atcagggatc gagccgagag cgctcccgcg accagcagcg cggcgagggc cgtggctccc
     5521 gccaggttcc agcggaacgc atgtggcgcc cagagcgcgt cgatcgcccg ctgccaggcc
     5581 gacggcctct ccgccgtccg ctccatcacc cgcgccacga actcatgggt cgcggcgggc
     5641 cggggcagat ccgcgacgcc gagcatcagc cgctccagct cgatcagccg ctcgcgcagc
     5701 gcctggtcgc gtccgagctg aacgagcagc tcgatgcgct cttcgccgga gagctcctgg
     5761 tcgatgaatc gttgaacgag cagctccgag cctgaagacg ggtcgtactc tctcatcggc
     5821 gtgctccgtc tactggtgtg tccttgcgac ttctggagcg gacctcccgt tggtcggccc
     5881 gcgaaatctc ggtcgagggg ctgtcgttgg cacggtccac cagatgggcc agcgttcggc
     5941 agagaatgtg ccgcgccttg tagacgcgca tcttgagcgt atcgcgatgc acgccaagaa
     6001 tggtgctcat ctcgtcgtac cccagacctt ccacgtgtct cagcacgaac gattcgcggt
     6061 agaggggcgg cagcgcctgg actgcctggt cgagttgccc ggccagctcg ccctgctcga
     6121 gcgcccgatc gggcgtgtcc tcgtcggcag gctcccaggc ggcgccttcc tcggcgccgg
     6181 catcgatcga caccgtaccc ggacgccgtg tccgaaggac gtccgtgcat ttgttgaccg
     6241 cgatccgata gagccaggtc gagaacttgg actcgagctg gaacccttga agggccttgt
     6301 atgccgccac gaacgcctct tgcgcgacat cctctgcatc ggccgtcgag cccagcatac
     6361 gggtcgttac gctgaggact gccgatgcgt gcctcgagac gaggatgccg aacgcatccc
     6421 ggtctccacg ttgcgacgct cgcacgagcg acgcgtcgtc ggcggcctca tcctgcgcca
     6481 tcgataacta gaaccttcgg ctcgctccaa gcatgaaccc gtgatcgggg gccccgtcgg
     6541 acaacccgaa cccgcccgac acctgcaggc gccagagtgc gcccttcacg gtaacggcgg
     6601 ccaggatgtc acgggcattc tcgaggcccg gcacgatggt gctgtactcc tcgaagaaca
     6661 cgctcacgtt gaccgcaccg tcagcgaggt cctggccgac gcccacgtcg taccaccaac
     6721 cgttcctgta ctcgactccc gtcggctttc cgatgatcgt gtacccggcg tcgagcatcg
     6781 tcacgaagcc gcctcccacc atgcggctga tttcgacgcc gacgccctca tcgggccgcc
     6841 ccgtaccgag gcccttgccg ggcgcggctg tcggcacctt gaggtgggct cgaacggcaa
     6901 tcgtcggcca ccacgatcgt tcgtcgagga cgtagtagcg gccgcggacg ccgatgtcgc
     6961 ccgcaccgca ggtcgtgccg ggcggcgtgg cggtgtctgg ggcggccgcc gtcgtcgtgg
     7021 cgcccgccgc tccagttctc gtggccgggt cgctcgtcgt tgttcctgtt ctcgtcgtcg
     7081 tgccggatga cgtcgcgcca gttcgcgagg tgtcaattgg catcggcgtg ccatcgacga
     7141 tggtcaccgc accggtgccc ctgaggcaca tgatcgggaa cacgagcgtg agatcgccgt
     7201 cggtgaacag ccgtctcgct gtgaacggcg tgtagaacac gtccgtgcga gagctggtgc
     7261 cgtaggtgcc gctcgtgaag ctcggcgtgc tgcccacctg ccactgctcg tcctgggcgc
     7321 ggctagacgc cgggacgagc agcagcagga ggaccgccgt caggatgcat ccggaagtct
     7381 tcaaggatcg tcaccctcga ttcggttgcg ccgtgcgctc gattcgttcc ggccgatcgg
     7441 gtctggcagg acgttccggc cgttccggac gctccggcct ggccgcgcgc tcgcgccgct
     7501 ccggccgatc aggtcgtgcc ggccgatcgg ggcgccgttc gtcgcgacgc tcgcggcgat
     7561 cgactcgccg gtcatcgcgc ctctcgcgcc gatcgatccg ccggcgatcg cgatcccgat
     7621 cccggtcgcg tccatcgtcg gcagtcggct tgggttcggc ctgcgtcgtt ggctcggtct
     7681 gtgacgtctg cgcgaacgct gacgctccac tcatcgccgt gatgcacccg gccgcgaaca
     7741 gcgccgggat gatgcctcgt cgcttcatga ctcctcctcg tttatcggtg tcgcctcgct
     7801 cgacccgcac gctccagtgg gatccgcacg tgcaacgatg agcaggtcgc agcagaacct
     7861 ctccaccccg tgaaggatgg agcgtgtccg tcacttgatt ggacgaggtg ggaggcagtg
     7921 cgtaaacggc cgggccgtga agatggcgcg attcacgaac tcggaacagt gaacccggaa
     7981 cccggaaccc ggaacacgaa cgtgaaactg aacacgaaca gagaagtgag aactgagaaa
     8041 ggtgaacgac agatctctgg ctcagttcag gtgctgcgac gttcagagaa acgcacgaag
     8101 aaccgtggag acgttcaact cgcgccgccc gagcgcgacg tccatgaacg tcggaacgag
     8161 cgacggccgg cggagcagtg cgcgcgccgc gcgaccttcc gcgcgcgaga tggccagcag
     8221 cacgcgcccg aggcgatcgg ccgtgcggcc catcgcgagc cgtgtgcgtt cgtacgacag
     8281 gagatcgccg gacacgatgg cgtccgccgc cggccgcacg gaggcgagtg ccagcgcgat
     8341 gccgtcgccg gtgatcggat ccggggcgcc tgccgcatct ccaatcagca gcagattgtg
     8401 atcgactacg cgcggcacac gcagcccgag cggcgccgcc gcgagcactg gcgtcgtcca
     8461 ctcgatgcgc gacgtccgct ccctcagctc ggggatcgcc ttcagcagat gcgtgaggcc
     8521 gtcgcgacgg aagcgctcct gatgaaagag cgcggcgacc agcgcttcac cgccgcctgt
     8581 cggcgccacg tacacttcgc cgtcgcgatg ataaaaaacc tcggcgcgat cgccgaggcc
     8641 gtcgagcccg ccggcgtgag aggagagtcc gacgcggcgg cgcgccggcc ggtgagcgct
     8701 cagccgcttg tgaaacacgg aatgcctccc gtcggcgccg accgtgaagc gctcgccgcc
     8761 gtccctgtat ggcgtcttca tacgggcgtc cacattcggc gtcgccgcgg ctctctcgaa
     8821 cagccacgcg tcgaagcgat cccgccggac gatcagcccg aaggcgtccg ggaagtcggc
     8881 ctcgaccgac gcgcctccgg ccacgaacct gaggcctcga atgcgcggga agtccgggag
     8941 ccccgcgatc ccggcgagcg cggcgactcc gtgcgggagc aagccctcgc cgcacgcctt
     9001 gagccgagga tgttgtgcct tctcgtagag gttgacgcgt acgccgcgtc gtccgagctc
     9061 gatcgccagg gccgccccgg caggtccgcc gccggccacg gcgacgtcgg ctaccacgac
     9121 aaggccagca gctccgtcgc gaacccggga ccgagcgcca gcacgagacc ggtgtcgccg
     9181 gcgcgaggct tgccgcttgc gacgagattg ctcagcatca cgagtacagc agcgctcgac
     9241 aggttgccgt accggcgcat gcagtcgcgc gccacggcaa gggcgtcgtc agggagcccg
     9301 aacgccgatc gatacgtcgc catgaccttc ggcccgcccg ggtgcaggac gtggtgcgtg
     9361 atctccttca ctgtcatccc ggcattgccg aggaactcgt ccacgagcgg cgcgacgcgc
     9421 tcgcgaacga gggatgggat gtcgcgcgag aggatcagcc gcatgccgtc gctcgtgaag
     9481 cgccagccca tgagatgacg cgcgtccgga aagagtcgag agcgcgttcc gacgacgcgg
     9541 atgccgtccg gcgcggtgtc gtctccgccg acgagcgccg ctgccgcgcc gtcgccgaac
     9601 aacgcgacgc ccacgagatc ggtgggcgtc tgtgctcggg gtgagaagat catgctcccg
     9661 agctcgaccg acacgacgag cgcgcgctct cgcggctttg ccgtgaggac gtcgcacgcc
     9721 ctgaccagcg cacccgcgcc cccggcgcaa cccaggccga agagcggcga ccgccggacg
     9781 tccacgcgca ggccgaggca gcctgcgagc agcgcatcca ggctcggcgt ggcgagcccc
     9841 gtcgtcgtca cgaagtagat gtggtcgatc tgatcgggcg cggtcccgga gctggccagg
     9901 cactggcgcg cggcgcgctc ggcgagctcg agcgcgcggg cggcgtactc gtcgttgcgt
     9961 tcctcgaacg atcgcggcgc cagataccac tcgggcggac gcaccaggtg ccgcgtttcg
    10021 acgccggcgt gatcgaagac cgggagcagc cgctcgaggt gcggctgtcc ctcgcagatg
    10081 cgcgccatct cgcgccgggc gaacgactgg tcgagacgat gcggcgggac ggcggtggcc
    10141 agggcctgta tgtgtggcat cgggcgcgcg ggagtcgacg accgcgtcga atgccgacga
    10201 ttctttcaca ctattcggca gtgcaccccc acacgcccgc accctcgcac ccccgctatg
    10261 catctttttg gacagaaggt cggtagcttt ggagggcgcg gcccgatctc gactgttttg
    10321 ctcgcctccc gagaagcaga acgcaggcac gagtattgcc acagccccct cgcccctctt
    10381 caaggagagc tacgtggaca attcacgccg tctgatcgtg tcgttcggcc cgagccagga
    10441 cccgccttct accagctctc gacggcagtt ctgcgcgcac gcctgccagg cggtttcgct
    10501 cgttgcggtc ggtgcgctca tccccgcatg cggcggtgag ggcgatccga atggacctga
    10561 ccctgtgccc gacggcaact cgccgctgcc gctcctgagc ggaacggcta ccggaaacac
    10621 cgtctcggtg ccgaccgacg gtgctctggc cgccgtcggc agcgcggcgc tcgtgtcgag
    10681 cccggtcggc agctttctcg tcgcccgcac cgcgcagacg acgttcagcg tcctgactgc
    10741 cgtgtgcacg cacgagggat gcaccgtcga tctcttcaac ggcgcgctgt tcgtgtgccc
    10801 gtgccacaac tcgaagtaca cgaccgccgg cgcggttgcg aacggaccgg cgacccaggc
    10861 actccgatcc ttcccgtcaa cgctgacggg cacgacgctg acgtttactg tctgatcttc
    10921 gatgctgcct gaatcgagaa atgagatctg ccgttcaccc ttctcccttc tctgttctcg
    10981 gttcgtgttc aatttcaggg ttcgtgttta aggttcgcgg ttcggggttg acttgttccc
    11041 ggtgcgtcgg tgaacccgga acagcgaacc cggaacacga acctgacact gaacacgaac
    11101 cgagaagtgc gaacgacagc tcgcctgctc aacgcaactc tcgacggtac gtctccttca
    11161 accacaacga tgacacgaag gagagcagcg acatgacggt gatgtatgcg gtcacgaggc
    11221 gggagttgcc ggcggcgttg tagagcgtcg tggcgatcac cggtgcaagc gcgccgccga
    11281 ggatcgtgcc gagcgtcagg ctgagcgacg cgccgctgta gcggatcgcg gccgggaaca
    11341 actccgagaa gaccgcaggc tgcgggccga tgtaggcgcc ctgtacgatg aacattccgc
    11401 acagcgccag cgcaatcagc ggaatcgact tcgtgtcggc gagcggaaag aacaccaacg
    11461 accagaggac gagggctccc aggccccact gcgtgatcct gcgtcggcct atgcgatcgg
    11521 accagcgcgc gaagacgacg atcgagccgg cgaacagcac ggcacccgtc agcagcaagg
    11581 tcagcgtcgt cgggagcggg agacccagtt cccgcgtcgc gtacgagacg aagtacacga
    11641 tgacgacgta cccgttggcg ctgatgccga cgtagctgcc cgccgcgatc aggaccgcgc
    11701 gccagtcgtt ccgcaggacg tcgaggatcg gcatgcggcg tgccgatgcc gttcgcagca
    11761 cctccgcgaa gaccgggctc tccgacagcc ccaaccgtac ccacaggccg acaaccacga
    11821 gcgccgcgct tgcgaggaag gggatacgcc aggcccaggc cgcgaactcg tctggttcga
    11881 agaggcccga gatgacgaga aacaccaggt tcgacaggat gatgcccacc ggcagcccca
    11941 gggcaacgaa gcttccgaag aacccgcggc gtccacccgg tgcgtgctcc accgacatca
    12001 gaacggcgcc gccccactcg ccgccgagcg cgaacccctg gacgaaacgg aggatcacca
    12061 gcagtgccgg ggcccagacg ccgatgcgct cgtagtttgg aagcaggccg atgccgagcg
    12121 tggcgagccc catcagcatc agcgaccaca ccagcatcga cttgcgcccc agacggtccc
    12181 cgtagtgacc catggcgatg ccgccgagcg gccgtgcgat gaatccgatg gcgaaggtgg
    12241 caaacgccgc gagccgcccg gccagatccg aggcctgcgg aaaaaactgc ggcgcgaaca
    12301 cgacggcggc cgccgtgccg tagatgaaga agtcgtacca ctcgatggtc gtgccgacgc
    12361 ctcccgcgag cgcgacgcgc cgcgcttcga ccgggttgtt acgtgccgca gacgccggtg
    12421 ccgtggtcat gtgccgggat attacccgta tactgatcgt tcctggaggg aatagccggc
    12481 atgagcagca caccttcgcc gcagcgtcgt ctctggttcg tccttgtcat cgcgacgctg
    12541 gtcgccgttg gagttcacac gggcgccggt caacagacgg tgcccttcca gggtggcatt
    12601 ccggtcgcgc cctcggggct ggccggtcgg aagctgccga acctgccgat ggagtttccg
    12661 acggcggaag gacatcgcat ccgcgtcgtg gccgtcacgc gtgcgctcga attcccgtgg
    12721 agcctcgcca ctctcccgga cggaacgatg ctcgtcacgg aacgttccgc gcgcttgagg
    12781 atcatccgga acggcgtact cgatccccag ccggtgcccg gaggtccgac ggggtatttc
    12841 accggcgagt cggggctgcc cggagccgtc cacggctaca tggatgtcgt gctgcacccg
    12901 cagttcgcgc agaaccggct cgtgtatctc tcctacacga agccgatcga cgagaagcgg
    12961 agaactgtcg gcattgcgcg cgggcgattc gacgggcgcg cgctcaccga cgtcaaggac
    13021 atcttcgtgc tcgaccaggc cggcacgtcc cgcctcgcgt tcggccgaga cggcatgctc
    13081 tacgtcacca cgaccggcca ggatccgcag aacgccaaca cacagggcgg aaagacactc
    13141 agactccgag atgatggcac catcccgaag gacaacccgt ttgccgggcg cgccaaccac
    13201 aagccggaga tctttacgct cggccaccgg aactcgctgg gcctggcgat gcatcctggc
    13261 accggcgaga tgtggcaaaa cgagaacggc ccgaatggcg gcgacgagat caacatcctc
    13321 aagccgggcg ccaattacgg ctggccggtt gtgagctacg ggcgcaccta tcctggcccg
    13381 tggcaatccg agcgcccggg acatgtgggg ttcgagccgc cgatcgtgta ctggatgccc
    13441 gcgattgccg tgtcgggcat ggcgttctac accggcgaca gattcccgaa gtggaagggc
    13501 gacgtcttcg tgggcgggct gcgcatgggc gagatcccgg gaaccggcca catcgagcgg
    13561 atcctcttca acgagaagat ggaggaactg cggagggaat ccctgctcgt cgagctgcac
    13621 cagcgtatcc gcgacgtgcg gcaagggccg gacggattgc tctacgcgct gacggacgag
    13681 aaggaaggca ccctgcttcg aatcgagccc gtgaactgag ctttcccgag gaggtatcgt
    13741 cgtgatgcgc agcaagccga tcgatggttg gcggtggcgg atccacgcat gcgcgttcct
    13801 ggtggccggg ctcgcctggg cggctcatgc ccagcagacg ccggcgaacc cggatacgtc
    13861 gagtaacttc accggcggaa aggtcacgac gctgatggga gaaggacgca tttcctatta
    13921 cgtcttcgag tcgggtgcgc ggaccaggtg gcacagccac gagggagggc agctgctgct
    13981 ggccgaagag ggtatgggac gcacgcaggt caggggcggc gccgttcgcg acctgcgccc
    14041 cggcgacacc gcgtggtcac cgcccggcat cacgcactgg cacggcgcct cgccaggtca
    14101 ctccgcgaag atgtaccagg tcagccgcgg tatgacgacg tggatggaag ctgtcagcga
    14161 aggagactac gcggggaagt aggcagtgag gtcgactcac tgcctactcc ctactgcccc
    14221 ctgccccctg tcccctgccc cctacttgat gtaccagtgc gctgcgacgc tgactgagaa
    14281 tccgccgagc tcgtggccgg caaacggcga ctcgaaccag tggtagccca tgtcggcgct
    14341 gacggcgagc tgcggcaggc tcgccacgct gaattcgcct ccaccgaacg cctggaaccc
    14401 ggcgttggaa tccgacactg gaagcccgcc gaacggcgtc atcgtcgagc gattgaacgt
    14461 cgcgccggcg cccgcgtacg gacgcagcca gatgtaatcg gagatgcgat caccgaacgt
    14521 gtacagcgcg ctcgggccga actgcgtcga cgtcatgacc gtgctggtcg ggtcggtctg
    14581 cgagaatcgc gacacgtcga actgaacgcc gagcggcccg cgcgaccagg ctcgagcgct
    14641 ggcgccgagg ccgagggccg atccgcccag gcgcgcgccg agtccgatct ggtgcctggg
    14701 tgtcgagatg ttcgaggacg actgcggacg cggcggcacg gtgcccactc gctgcggctc
    14761 ggcgttcgct gaggttgtca gtcgaggcgc cggagcggac gcaggctggg cggccggtcg
    14821 ggacgcgctc gaggcggcac gcgccgcgac aggagccgcc ttgccgatcg agcccgcgtt
    14881 cgtgcggaca tacccgctgc cgtcggccgc cgacggccag gtcactttca cccagtctcc
    14941 gacttcccgc gtcacctcga gcgtcgtgcc ccgattggca cggccgatca cggggcttcc
    15001 cgcgctcggg gacttgtgca cgctcgccga cgcggcgttg atcgtgattt cggtgttctg
    15061 cgcggcgacc ggcgccgtga acagacacag agcgacgacg atggctgcag cgtgacgaat
    15121 catcttgcct acctcctgct cttgcgaccc ccgaatgtgt cgccggaatg accgttccgt
    15181 gatcgccggt acgcgtggat gcacggaaat ggccgtccgg ggccacctct cgaacgaggg
    15241 agcaaatgcc atgcttggcg cgccggaaag agggaggggc tgtactattc tggcgtgatc
    15301 gaggcccgga atctcacgaa gcaatacggc cgggtcacgg ccgttgacgg cctgtcgctc
    15361 acggttgccg aaggagagct cgtcgtcctg ctcggcggtt cgggctccgg caagacgacg
    15421 acgctcaaga tgctcaaccg gctcattgag ccgacctcgg gtcgcgtggt catcgacgga
    15481 caggacgcgc tttccgtcgc gccgcacgag ttgagaaggc gcgtcggcta cgtgttccag
    15541 cgcgtcggcc tgtttccgca catgaacgtc ggcgagaacg tggcgatcac gctgacgctc
    15601 atgggatggg atcgcggccg tgcggccgcg cgcgtggagg agctgctgga gctcgtggag
    15661 ctcgacccgg cggcgatgcg cgaacgccgc cccgccgagc tctccggagg cgagcagcag
    15721 cgcgtgggcg tcgcgcgagc gctcgcggcg tcgccgcgcc tgatgctgct cgacgagccg
    15781 ttcggcgccc tcgatccgct gacccgcgat cggctgcagc agtcgttcct gcgcatccgc
    15841 cgtacgctgc ggctgacggc catcttcgtc acgcacgaca tggtggaggc gctgctcgtc
    15901 ggcgatcgca tcggcgtgat gcaccacggt cgcctcgtgc aggtcggcac gccctcgcaa
    15961 ctgctgcagg cgcccgccga cgactacgtc cgtcagttga tgaacactcc gaggcgccac
    16021 gccgctctgg tggacagcct gctcgctcgc ccttcgactg ttgctcaggg cgtcccgagc
    16081 tccgtcgagg gacgtgattc ggagtcctcg tgaaggaaca gctcgcgctg ctgcctcaat
    16141 acgtcaccgc gcacctgcag ctgacgttgt tcgctctcgc ggccggaatg gcgttcagca
    16201 tcccggccgg catcgccatc acccggtcgc cgcggttcga gcggcccgtg ctcgcggtgg
    16261 cgagcgtcat ccagaccgtt cccagcctgg cgctgctcgc catcatggtg cccgccctgt
    16321 cggcgctcgg gctcaggagc atcggcttcc tgccggcgct gattggcctg agcctctaca
    16381 gcgtgctgcc gatcctgaga aacaccgtga ccgggttgtc agggctggat ccggcgttga
    16441 ccgaagccgc acgcggcgtc gggatgactc cgcgccagca gttatggcgc gtcgagctcc
    16501 cgctggcgat gcccgtcatc ctggccggcg tgcgaacggc gacggtctgg accgtcggca
    16561 tggcgacgtt gtccacgcct gtcggcgcgg cgagcctggg caactacatc ttctccggtc
    16621 ttcagacacg caacctcacg tcggtcctcg tcggctgcgt cggcgccgct gcgctcgccc
    16681 tcgtgctcga cgggttggtg cgcgcgctcg gggccgctct cgtcggccgg cgtcgttcgc
    16741 cgctcttcgt cgtcctggcc acgctgggcg tgctctacgc gtactcgtcg gtcaccttcg
    16801 tccaggggct tcgtcgaagc ggtccggacc ggatcgtggt cggcgccaag acgttcaccg
    16861 agcagtacat ccttggccgg attctggctc ttcagatcga gaagtccacg gggagccctg
    16921 tcgaggtcgt gtcgtcgctc ggttcgacgg tcgttttcga tgcgcttcgc gcaggcgaga
    16981 tcgacgccta tgtcgaatac tcggggacga tctgggccac gatcatgcat cgcgattcgg
    17041 cgccgacgac gcgggccgcg ctgctgagcg aggtcagccg ctttctcgag tcgcagcacg
    17101 gcattactgt ggccggccca cttgggttcg agaacgctta cgctctggcc atgcgacgcg
    17161 gacaggcgac cgggctcgcc gtgaagagca tcagcgatct ggcgaggcac gcaccgaggc
    17221 tggcgatcgg cggcgactac gagttcttcg accgtgccga gtggcgcgcg attcgcgaca
    17281 tctacgggct cgagttccgc gaccagcgaa gcatggatcc gtcgctgatg taccaggcgg
    17341 tcgccaacgg cgaggttgac gtgatcggcg cgttctccag cgacggccgc atcgcggcgc
    17401 tcgatctcac ggtgctgcag gacgagcgcg gcgccattcc accgtacgac gccatcatcc
    17461 tcgcggggcc gcggctcgtc cgcgagcgtc gggacgtgct ggccgccctg agcgcccttg
    17521 ccggacggat cgacgcagac gaaatgcggc ggatgaatct ggccgtcgat caggaaggcg
    17581 ccgccccgga tgacgtggcg gcgcgatttg tccgcgccga atcaccataa ggcgccgtag
    17641 agcgcgggct tcagcccgcg cggactaagc tgtgatcatg agcgatccga tccttcacgt
    17701 gaaacccctc gggcatcggt ggcagacaga ggacccgttc ctgttctgcg ttcaccatat
    17761 cgacctctat cccaagggaa acgaacagtt cggtcccgcg gccccgctcg aaggccgcaa
    17821 catcggacag gactttgacg ggctcgacgg ctggcggatg taccacggcg acgtagtacc
    17881 cgggtttccg cagcatcccc atcgcgggtt cgaaaccgtc acgatcgttc gcagcggctt
    17941 catcgatcat tccgattccc tcggcgcggc ggcgcggttc gggtcgggcg acgtgcagtg
    18001 gctgaccgcg ggggcgggcg tggtccattc ggagatgttc ccgctgctcg accgcgaggg
    18061 cccgaatacg ctcgaactgt ttcagatctg gctgaacctc ccgtccaagg acaagctcgt
    18121 ggacccctac ttcacgatgt tctgggaccg cgacgtcccg cggcggcgct tcacggacgc
    18181 cgcgggcggc gaggcggaag tgacgatcat tgcgggcacc ctcgatggca ccacgccgcc
    18241 ggtgccgccg ccgcactcat gggcgtcgag gcccgagtcg gacgtcgcga tctggtcgat
    18301 cgtgctcgag aagggcgcga gctggacggc gccgcccgcg aagaccggat cgacgctccg
    18361 cacgctcaac ttctttgccg gtaagtcgct gcgcgccggc gaccgtgtgg tgtccggcct
    18421 ctcggcgatt caggtgaggg cggatcagcc agtagggatc gaggctgttg acggccgtgc
    18481 cgagatcctg ttgctccagg gccgtccgat aggcgagccg gtggcgcagt acggtccgtt
    18541 cgtcatgaac acgcgcgacg agctccagcg cgcgttcgcc gactatcagc ggacgcagtt
    18601 cggaggttgg ccgtggaagg aaagcgcacc cgtacacggc ggtcgcgacg ccggccgatt
    18661 cgccaggcac gccgacggcc gcgtcgagcg ggtgggataa atggggtcgg gagtcatttc
    18721 ttgaggacgc ctcgcactca agaaatgact cccgac
//
LOCUS       DBNGSH010000438         4178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1228134, whole genome shotgun sequence.
ACCESSION   DBNGSH010000438 DBNGSH010000000
VERSION     DBNGSH010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1228134"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC16CY_10010"
     CDS             complement(<1..529)
                     /locus_tag="AC16CY_10010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3354166.1"
                     /translation="MSTRLFRSFVCCLTTALSLLTVAVEARVQARTVRMAGPYGGQRV
                     FAILLPQSYDTSEARYPVVYLFHGGGQDHTAWMARTAFAPMARRHEFIVVMPAADRGY
                     SGLGSEAQGQYHDYVAGDLVDYVDSHYRTIPTREGRAIAGLSMGGRIAAMTALRHPDR
                     FAAVGAFSAALRPDVE"
     gene            complement(589..1056)
                     /locus_tag="AC16CY_10015"
                     /pseudo
     CDS             complement(589..1056)
                     /locus_tag="AC16CY_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            complement(1157..>4178)
                     /locus_tag="AC16CY_10020"
     CDS             complement(1157..>4178)
                     /locus_tag="AC16CY_10020"
                     /inference="COORDINATES: protein motif:HMM:NF019291.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HIR3354167.1"
                     /translation="SIAIVTPWGARLEGVWRSAVVEAIGADARWCFCSNGLVLRLVDA
                     RRTWSRDYLEFDLQTLGRDVEAQAVLWAVARGEAMASEPPLLDRAVDLSIRHGVEVCR
                     ALGVGVLEALEVLLGAMARGGSRSASVLWEQSLTVLYRVLFLLFAEARGLVPLWHPVY
                     RERYSVETIAATLLDGRPCRGLWRAMQAISRLAHSGCTAGELRVTAFNGRLFAPAQAA
                     AFDGRRVSDEALSRALMSVSSTPVDRRGGRARIAYRDLDVEQLGAVYERVLEYEPSTC
                     RPARHSIASSGPVSLTRTRDARKSSGTFYTPRAVTAFLVRRTLEPLVNGRDADGILTL
                     RVLDPAMGSGAFLVAACRYLASAAEQALVREGRWHAHDVTPADRASLRREIASRCLFG
                     VDLNPMAVQLARLSLWLATLAADKPLSFLDHHLLAGHSLVGATPDVIHRQPGGGRSTS
                     GRHRRPLPLFDDTGLPSALEHAARVLTKVSAEPDDSAAIVRAKERTVFELHARESSLG
                     KWTEVLDAWCASWFWSHGTPPDRGTFLDLMNRALGRPCALPHHVAGRLLDQSRDVASR
                     LRFVHWPLAFPDVFLDLDASNRPAGFDAVIGNPPWDMIRGDSGEGDARADRRAEARAL
                     TGFVRGSGIYQVASRAHLNLYQLFLERSLQLVAPGGRIGFVLPSGLVSDTGAAPLRRH
                     LFDRAEVDEITGFDNQGGIFPVHRSVRFVLLTCSTGRPTDAVRCRFGLTRPDQLDAPG
                     RPLVLSRRFLARLSGEDDLGIPELASERDLAIVEGISAVTPRLGSNDGWHAEFGRELN
                     ASDDRGLFVPISRGAPRRPVIEGKQIEPFRALVGDCRFELRPGAQTGRRVGRRARLAY
                     RDIASATNRLTLIAAIVPAHAVTTHTLFCLRTPLPEAEQHVLCALLNSYVANYLVRFR
                     VNTHVTVSLVSRLPVPIVRRGDAFFERVSALAGALVDGAGPVEDREEHAELQAIAARL
                     YGLTEADFEHVLTTFPLVPERVRHAAREKFSGFD"
BASE COUNT          726 a         1427 c         1399 g          626 t
ORIGIN      
        1 tctcgacgtc cggccgaagc gccgcgctga aggcgccgac tgccgcgaac cggtccggat
       61 gccggagcgc ggtcatcgcg gcgatgcggc cgcccatcga caatcccgcg atcgcgcgtc
      121 cctcccgcgt gggaatcgtc cggtagtgcg agtccacata gtccaccagg tcgccggcaa
      181 cgtagtcgtg atattgcccc tgagcctcgc tgccgagtcc gctgtagccc cggtccgcgg
      241 cgggcatcac cacgatgaac tcgtggcgtc gcgccatggg cgcaaacgcc gtccgcgcca
      301 tccaggcggt gtgatcctgg ccgccgccgt gaaacaggta gacgaccggg tagcgcgcct
      361 cggaggtgtc gtacgactgc gggagcagga tggcgaagac ccgctgcccg ccgtaggggc
      421 cggccatcct gaccgtgcgg gcctgaacgc gtgcctcgac agccacagtc aagagcgaga
      481 gagccgtcgt cagacaacag acgaaggatc tgaacaagcg agtgctcatc tcccagagtc
      541 tacgccttct ctcgtcacga accacggagg cacggagaca cggaggttct acaacattac
      601 tcggcggatt ccgtgcttca tcaccgctgt cttgaaattc aagagcaggc ccactcgcaa
      661 tttgagcacg cgcaagtagg taatcacctg cgcaacgtag actggttcca ggtgctgcac
      721 gcacttcact tcgacgacaa cgcgatccgc caccacgaga tcgggccggt gctccgagat
      781 gagccgccct ttgtaataga ggggcacgcc ttactgtcgc ctgtacgaca gaccgcattc
      841 ttccagttcc agacagagtg cagactcgta gactgattcc agaagaccag gcccgagttc
      901 cctgtggact tctaccgcgc atccaatgat ctgatccgtc aattcgttgt cgggaccgta
      961 aagcaaagaa tcctccgtgt ctccgtgtct ccgtgcctcc gtggttcgtg atacgtgccc
     1021 gtctcgtgat tgcgaggagc ggctcccgtc gcgcatgaat cgaattcctc tgtgtcctct
     1081 gtgcctctgt ggttgacgtc cgtgatcagt aatgtctgtg aattcctcgg tgtctccgtg
     1141 cctcggtgtg tcgggatcag tcgaagccgc tgaacttctc gcgcgccgcg tgtcgcacgc
     1201 gctcggggac taacggaaac gtcgtcagca cgtgctcgaa gtcggcctcg gtcagcccgt
     1261 aaagccgcgc cgcgatcgcc tgcagttccg cgtgctcctc ccggtcctca acgggccccg
     1321 caccgtcgac gagcgcgccg gcaagggcgc tcacacgttc gaagaaggcg tcccctcgcc
     1381 gcacgatcgg aaccggcagg cgcgacacga gcgagacggt gacgtgcgtg ttcacgcgaa
     1441 agcgaacgag gtagttggcg acgtagctgt tgagcagcgc gcacaacacg tgctgctcgg
     1501 cctccggaag aggcgtcctc aaacagaaga gcgtgtgagt ggtgacggcg tgagccggga
     1561 cgatggcggc gatcagcgtc aggcggttcg tcgcgctggc gatgtcgcga taggcaaggc
     1621 gggcgcgccg gcccacccgc cggcctgtct gggcgcccgg ccgaagctcg aagcggcagt
     1681 cgccgacgag ggcgcgaaac ggctcgatct gcttgccctc gatgacgggc cgcctcggag
     1741 caccgcgcga gatcggcacg aacaggccgc ggtcgtcgct ggcgttcagc tcgcggccga
     1801 actcggcgtg ccagccgtcg ttcgacccga gccttggcgt tacggcgctg atgccctcga
     1861 caatcgcgag gtcgcgctcg ctcgcgagct ccgggatgcc gaggtcgtcc tcgcccgaca
     1921 gcctcgcaag aaagcgccgc gacaggacaa gcggacggcc gggcgcgtcg agttgatccg
     1981 ggcgcgtgag cccgaaccgg catcgcaccg cgtccgtcgg ccggcccgtc gagcatgtga
     2041 gcagcacgaa ccgcacgctg cgatgaacag ggaatatccc gccctgattg tcgaagccgg
     2101 tgatctcgtc cacctccgcg cgatcgaaga gatgccgccg cagcggcgcg gcgcccgtgt
     2161 cgctcacgag gcccgaggga agcacgaacc cgattcgacc gccgggggcg acgagctgga
     2221 gcgagcgctc gagaaacagc tgatagaggt tgagatgtgc gcggctcgcg acctggtaga
     2281 tcccggaccc gcgaacgaag ccggtgagcg cgcgcgcctc ggcgcgtcgg tccgccctcg
     2341 cgtcaccctc accgctgtcg ccccggatca tgtcccacgg cggattccca atcaccgcat
     2401 cgaaccccgc cggcctgttc gaggcgtcca ggtcgaggaa gacgtcggga aacgcgagcg
     2461 gccagtgcac gaaccggagc cgcgaggcga cgtcgcggga ctggtcgagc agccgtcccg
     2521 ccacgtggtg cgggagcgcg cacggccggc cgagcgcgcg attcatgaga tcgagaaacg
     2581 tgccgcggtc cggaggcgtg ccgtggctcc agaaccagct ggcgcaccag gcgtcgagga
     2641 cctcggtcca ttttccgaga gacgattccc gcgcgtgcag ctcgaacacc gtccgttcct
     2701 ttgcccggac gatcgccgcc gaatcatcgg gctcggcgga aaccttcgtc aggacgcgcg
     2761 cggcgtgttc cagcgccgac gggaggcccg tgtcgtcgaa gagcggcaga ggccgccgat
     2821 gccgtccgga ggtcgagcgc ccgccgcccg gttgtcgatg aatgacgtcc ggcgtcgccc
     2881 cgacgaggct gtgaccggcg agcaggtgat ggtcgagaaa cgacagcggc ttgtcggcgg
     2941 cgagtgtggc gagccagagc gacaggcggg caagctgcac cgccatcgga ttgagatcga
     3001 cgccgaacag gcagcgtgag gcgatttccc ggcgcagcga cgcgcgatcg gccggcgtga
     3061 cgtcgtgggc gtgccagcgt ccctcgcgga cgagcgcttg ctccgcggcc gacgccagat
     3121 accggcacgc ggcgacgaga aacgcgccgc tgcccatggc cgggtccagt acgcgcagcg
     3181 tcaggatgcc gtccgcatcc cggccgttga ccagcggttc gagcgtacgc cgtaccagga
     3241 acgctgtcac cgcacgtggc gtgtagaacg tgccgctcga ctttcgcgcg tcgcgggtgc
     3301 gggtcagcga caccggtccg gacgaagcta tcgaatgcct tgccggtcta cacgtagacg
     3361 gctcgtactc gagcacgcgc tcgtacaccg ctccgagctg ttcgacgtcc agatctcgat
     3421 aggcgattcg cgcccggccg ccgcggcggt ccaccggagt gctgctcacc gacatcaggg
     3481 cgcggctcag cgcctcgtcg ctcacgcgac ggccgtcgaa ggcggccgcc tgtgccgggg
     3541 cgaacagccg tccgttgaat gcggtgacgc ggagctcccc ggccgtgcat cccgaatggg
     3601 ccaggcgcga gatcgcctgc atggcccgcc agagcccccg gcacggccgc ccgtcgagca
     3661 gcgtcgcggc gatggtttcg acgctgtatc gctcgcgata gaccggatgc cacagcggca
     3721 cgagcccgcg cgcttcggca aagagcagga acaggacacg gtagagcacc gtgagcgact
     3781 gctcccatag caccgacgcg gaccgcgatc cgccgcgcgc cattgcgccg aggagtacct
     3841 cgagcgcctc gagcacgccc acgccaagcg cgcggcagac ttcgacgccg tgacggatcg
     3901 acagatcgac ggcgcgatcg agaagcggtg gctcgctcgc catcgcctcg cctcgtgcca
     3961 cggcccagag gacggcctgt gcctcgacgt cacggccgag cgtctggaga tcgaattcca
     4021 ggtagtcgcg cgaccaggtg cgcctggcgt ccaccagccg cagaacgagg ccgttggagc
     4081 agaaacacca tcgagcgtcc gcgccgatcg cttccacgac tgccgatcgc cacacgcctt
     4141 ccagccgtgc gccccacggc gtgacgatcg cgatggac
//
LOCUS       DBNGSH010000439         2273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2417638, whole genome shotgun sequence.
ACCESSION   DBNGSH010000439 DBNGSH010000000
VERSION     DBNGSH010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2417638"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="AC16CY_10025"
     CDS             complement(<1..614)
                     /locus_tag="AC16CY_10025"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3354168.1"
                     /translation="MIPLRDVIPSRTTPFVTIALVVTNVLVFLYEFSLGPAVQDFMFT
                     FGLVPSLFSWPTVFTSMFLHGGFLHVGGNMLYLWIFGDNVEDRMGHGRFLVFYLLCGT
                     AAAFGQMVTGPDSRVPMVGASGAVAGVMGAYFVLYPHSRIVTLLPFIFIQIIEVPAVF
                     FLGIWLVIQFLSGVGSLATASSEEGAGGIAFWAHVAGFAAGLIGV"
     gene            692..901
                     /locus_tag="AC16CY_10030"
     CDS             692..901
                     /locus_tag="AC16CY_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354169.1"
                     /translation="MICKHCQTEIADKALICYRCGRSTTEPRVAPPAEGSLFEQRGRS
                     RAAIVVAVIMAVVVALATWYLFQTP"
     gene            902..1792
                     /gene="rarD"
                     /locus_tag="AC16CY_10035"
     CDS             902..1792
                     /gene="rarD"
                     /locus_tag="AC16CY_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR3354170.1"
                     /translation="MAGIWYGLAAYSLWGFFTLYWSLLPNVRALEVVCHRIVWAFLLL
                     AAIGARSWRTRGVSLSPRVVGLYGAAALLIGVNWFLYVYAVNAGLVVESSLGYFITPL
                     VNVLLGVAVFRERLRAMQWAAVLLALAGVVRLTAAYGAVPWIALCLACSFGSYGLVKK
                     KAPLSSLDGLTLETAVLVLPAALYLAVVQSTGTGAFLVGGAFTSLLLVGAGVITVGPL
                     LLFATAVRRVPLSVIGILQYISPTIQFLLGVFVFREPFTSAQLSGFVLVWVGLVVFGV
                     DGALARKTTRDLAVLDEGAI"
     gene            1807..>2273
                     /locus_tag="AC16CY_10040"
     CDS             1807..>2273
                     /locus_tag="AC16CY_10040"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3354171.1"
                     /translation="MPTTVRQAQRDDVHVVEELLIEAARWVDALGEVMWEEGELSPGR
                     IAAEVEKGQFFLAVVDGEIGGAIRFQLEDRLFWPDLAQSDSAFVHRLVVGRRHKGLGV
                     STVLLQWAVDRARAMGKRYLRLDCDASRPKLRALYERFGFRVHSFRQVGAYYVA"
BASE COUNT          354 a          719 c          771 g          429 t
ORIGIN      
        1 acgccgatca agccggccgc gaagcccgcg acgtgcgccc agaacgcgat gcccccggcg
       61 ccctcttcgc tcgaggcagt ggcaagcgag ccgaccccgc ttagaaactg gatcacgagc
      121 cagatcccga ggaagaagac cgcaggcacc tcgatgatct ggatgaagat gaacggcagc
      181 agcgtgacga tgcgcgagtg gggatagagg acgaagtatg cgcccatgac ccccgcgacg
      241 gcgccgctgg ccccgaccat cggcacccgg gagtcggggc cggtcaccat ctggccgaac
      301 gccgcggccg tgccgcacag caggtagaac accaggaaac ggccgtgccc catgcggtct
      361 tcgacgttgt cgccgaagat ccagaggtag agcatgttgc ccccgacgtg caggaaccct
      421 ccgtgcagga acatcgaggt gaagacggtt ggccacgaaa agagcgacgg caccaggccg
      481 aatgtgaaca tgaagtcctg gaccgccggg cccagcgaga actcgtacag gaacacgagc
      541 acgttggtca ccacgagcgc gatggtcacg aacggtgtcg tgcgcgatgg aatgacgtcg
      601 cggagaggga tcacagagta cagttaggtt actcctgatt cctgagaccg ccctgaggcc
      661 tgaggcctga ggcctgggcc ctgaggcctg aatgatctgc aagcattgtc aaaccgagat
      721 cgccgacaag gccctcatct gttatcgttg cggacgctcg acgaccgagc cgcgcgtcgc
      781 gccgccggct gagggctccc tcttcgagca gcgcggccgc tcgcgcgccg ccatcgtcgt
      841 cgccgtgata atggccgtcg tcgttgcact ggctacctgg tatctctttc aaaccccctg
      901 aatggcgggc atctggtacg gcctggccgc ctactcgctc tgggggttct ttacgctcta
      961 ctggtcgctg ctcccgaacg tccgcgcgct cgaggtggtg tgccaccgca tcgtatgggc
     1021 gttcctgctg ctggccgcaa tcggcgcgcg atcgtggcgc acgcgcggag tctccctgtc
     1081 gcctcgggtc gtcggtctct acggcgcagc cgcgctgctg atcggagtga actggtttct
     1141 gtacgtgtac gccgtcaatg ccggcctggt ggtggagagc agtctcggct acttcatcac
     1201 gccgctcgtc aacgtcctgc ttggcgtggc cgtcttccgg gagcgtttgc gcgcgatgca
     1261 gtgggcggcc gttctgctgg cccttgccgg cgtcgtgcgg ctgacggcgg cgtacggggc
     1321 ggttccatgg atcgcgttgt gcctggcctg cagcttcggc agctacggcc tggtcaagaa
     1381 gaaggcgccg ttgtcgtccc tcgacggcct cacgctcgag acggcggtcc tcgtcctgcc
     1441 ggcggcgctg tatcttgccg tcgttcagag caccggaacc ggcgcattcc tcgtcggcgg
     1501 ggcgttcacg tcgttgctgc tcgtgggcgc cggcgtgatc accgtcggac cgctgctgct
     1561 ctttgccacg gccgtgcgcc gcgtgccgtt gtcagtcatc gggatcctcc agtacatttc
     1621 gcccacgatc cagttcctgc tgggtgtctt cgtcttccgc gagccgttta cgtcggccca
     1681 gttgagcggc ttcgtcctcg tctgggtggg gctcgtcgtg ttcggcgtcg atggcgcgct
     1741 cgcacggaag acgacacgcg atcttgccgt cctggacgaa ggcgcgatct aagcttcggg
     1801 cgtcagatgc cgaccaccgt gcgtcaggcg cagcgggatg acgtgcacgt cgtcgaggag
     1861 ttgctgatcg aggccgcgcg ctgggtggat gcgcttggag aggtgatgtg ggaagaggga
     1921 gagctgtcgc ccgggcgcat cgcggcggag gtcgaaaagg ggcagttctt cctggccgtt
     1981 gtcgatggcg aaatcggagg ggcgatcagg tttcagctcg aagatcgcct cttctggccc
     2041 gacctggctc agtctgattc agctttcgtc catcgtctcg tcgtcggccg gcggcacaag
     2101 ggcctcggcg tgtccacggt actgctccaa tgggctgtcg atcgcgcaag ggcgatgggg
     2161 aagcgctacc tgcgtctcga ttgcgacgcc tcgcggccaa agcttcgcgc gctgtacgag
     2221 cggttcggtt tccgcgtaca cagctttcgc caggtcggcg cgtactacgt ggc
//
LOCUS       DBNGSH010000440         7750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_210361, whole genome shotgun sequence.
ACCESSION   DBNGSH010000440 DBNGSH010000000
VERSION     DBNGSH010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_210361"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="AC16CY_10045"
     CDS             <1..1232
                     /locus_tag="AC16CY_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR3354172.1"
                     /translation="FRRIQVEPLAHGGEARRDVLVEVEESDATVLDIGGGVEGRFLLR
                     TGAGGIAEEHFELAPRGYFQIGRRNLWGKNRTANLFTRVSLRSREETTVPISPTEAPE
                     VSYGFHEYRVWATFREPRAFNTQAEVLFSAIIEQAIRSSFDFNRREIRAEAGLRLSPV
                     YTVSGLYSFQNTKLFNVNIAPEDEPVIDRLFPRVRIAKVSGSIIRDSRDDPLDASRGS
                     LFIVNADLAARALGSEVGFIKTFAQGFFYRQLPSPRRLVLALGARVGAAYGFLRTVEG
                     ETVQDLPASERFFAGGDTSVRGFSLDRLGNERTITPTGFPTGGNSVVVLNSELRFNLT
                     SKLQTIGFLDAGNVFPSASDLSVIDVRPAAGFGLMYRSPFGPVRVDLGFNLDPQEFVP
                     GVTERGNVLHILFGEAF"
     gene            1229..1861
                     /locus_tag="AC16CY_10050"
     CDS             1229..1861
                     /locus_tag="AC16CY_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3354173.1"
                     /translation="MRARVLLLSPVLCLLCGGGQLDAQQLLDKVLARVGTRAVTLTEV
                     RAAIGLGLVEVRRGEDADAAGLQRVIERQLVLNEVGRFPTPDPTAAELAEQVAAMKSH
                     AGASYERLAASTGLDEAKLQELARQTLRIRAYTTLRFGTTAQVTEEEARKYFDDHPEQ
                     FTREGARLSFEQAAPAARQRASAERLRATTDEWVRDLRARADVVIVGSGR"
     gene            complement(1883..2629)
                     /locus_tag="AC16CY_10055"
     CDS             complement(1883..2629)
                     /locus_tag="AC16CY_10055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3354174.1"
                     /translation="MSSEPLPATPGPGLSRSQQRVVAVIEVLMCSSVPTQVLIAGLLG
                     ALGLAPRTPDDQLSLPFVFALSLADSALLIALMVVFMRAHGESPWLLWRGRHRLVPEV
                     SFGLALVPLTVVAAGLLMTSLLQFFPSLHNVPMNPFETLAAGGYGDAAMMGVIAIVAG
                     GLREELQRAFLLRRFEQHLGGAAVGVVVLSTAFGLGHVMQGWDAAVTTGLLGAFWALL
                     YLWRRSSIAPILSHAGFNSIEVLRVAIGVS"
     gene            2766..4169
                     /locus_tag="AC16CY_10060"
     CDS             2766..4169
                     /locus_tag="AC16CY_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3354175.1"
                     /translation="MPQTNSDRVLIVEDEPSTRVGLTELVRTWGFTTDSASDGEEALQ
                     RITAFRPSIIISDLVMPRCDGIELLRALKEDGGDYTVVILTAQGTVETAVEAMKEGAY
                     DYLTKPIEPQRLKIVLDKIVERQDTLREVKVLRKQLRDHGTYGKMIGSSPQMRKVYQV
                     IEQAAPTSASVLISGESGTGKELVAQTIHQLSPRAQMPFVPINCAAIPETLLESEIFG
                     HEKGSFTGAVDRRAGCFELADRGTLFLDEIAEMTPSTQVKLLRVLQERKFRRLGGRNE
                     ETVDVRVIAATNQNPGDAVKNGKLREDLYYRLNVFAIELPPLRLRKEDLPLLVQSFLG
                     DFNSRNGKKVSALDQAAMRILEQYNWPGNIRELRNVIERAVILSAGEFIDPKLLPPLV
                     TESPDVVKPTLSLTPGTTVEEAETRLILMTLEHTRDNKTRAAEILGISLKTLHNKLNK
                     LRGRIPGKRSEEESVPQ"
     gene            4176..6143
                     /locus_tag="AC16CY_10065"
     CDS             4176..6143
                     /locus_tag="AC16CY_10065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3354176.1"
                     /translation="MRLSIRVKQIAGVTAIVGLAVVALSAFYTARLANVVLEESHSRG
                     QMLANTILHRAGSIVTGDVDPYAALRDDPGLRSILESSIYGESISYAAIGDTDGRAIV
                     HSITSMVGNTLPVRTDLVTIVADSPVEKMRVIFAGDGQTLEVRVPLTRGGEAFGSIRI
                     GVSTLFLRGHLRKLLTDAGFTALGALAVAIFVAGVLSQLLLRPIHVLRGGLTRLGKGE
                     FGVTLDMPSGDEFGELGDFFNTVSQQLSADRTLLAGQKANLQSAVEHLEDAVALFNPS
                     GELLFSNPAMQHTLPGDAIGRVLDGLLPTGHPYRTIVEETLATRKSRGPVQIPGHSTA
                     QIVVPGRQPPETAGAQALAGTTARDGRPADEELILTNAIAGTHGELVGVLLVSRNLAS
                     LSRMQSTLAYSRKLVALGRLTAGIAHEVKNPLNAMMIHLELLRTKIRGTFVPAMQPEP
                     VAAGGTLGLGPARAAPLPPPVQGALEHVEIIESEIRRLDEVVQGFLKFTRPEDLRLQP
                     VRAAALFEEIAPVIETEAGKHGVKVVIDVSAGTPNVNGDASMLRQVFLNLAINACQAM
                     PSGGTLRLVAAAASRNRVEVRVEDTGVGIKPEHLSRIFDLYFTTKERGTGIGLSMVYR
                     IIQLHDGEIEVQSTPGRGTTFRVLLPRAQGM"
     gene            6145..6732
                     /locus_tag="AC16CY_10070"
     CDS             6145..6732
                     /locus_tag="AC16CY_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354177.1"
                     /translation="MAVSSRLSGWAAVLLGVAMTASLSAGCAKARAATVPDGPPLAMP
                     LPPPRVFSPLEEEPLAASPAVAEPAASGAPQVTQKQDRAANRPSREPEKAEAPPPPAP
                     APVVEPARELRAASTPADAEADRKIRLLLNAAKGNLEKVNYGKLSTAGKEQFDQARNF
                     ATQGDEALTQRNYVFAETLADRAAKLATELLGLSR"
     gene            complement(6797..>7750)
                     /locus_tag="AC16CY_10075"
     CDS             complement(6797..>7750)
                     /locus_tag="AC16CY_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR3354178.1"
                     /translation="HAAVSPDDLTPSPIKRFFASEQWLPVLNYEDGYGLTYGVQFGFA
                     NSLGDRSRLSIPLTWGGERRAALEVERSFDRGPFSLVSGALSIDRRVNPHYDASDLRQ
                     GARVAVERSLHPWLRVGADGRVEHVDFGGIYDARHWAGGVHVTLDTRIDPSFPRNAIH
                     VRVGWERLAFPDGSAGRWLADARGYVGVGGSAVLALRAQVATSNHPLPAPEQVLLGGK
                     ESLRGYRAGYAANDNLSAFSAEVRYPLTSPLNVGRFGVKAFVDAGTTWASDGPGLKEQ
                     RFERGIGGGVYFGIAVLILDLDVAWPESGNPRVHFGFGITF"
BASE COUNT         1346 a         2574 c         2653 g         1177 t
ORIGIN      
        1 tgttccggcg catacaggtc gagccgctgg cccacggtgg cgaggcccgg cgcgacgtgc
       61 tcgtggaagt ggaggagtcc gacgcgacgg tgctcgacat cggcggcggc gtcgaaggtc
      121 gcttcctgct caggaccggc gcgggaggca tcgccgaaga gcacttcgag ctcgcgccgc
      181 gcggctactt ccagatcggc cggcggaatc tgtggggcaa gaaccggacc gcgaatctgt
      241 tcacgagggt gagcttgcgt tcgcgcgagg agacgacggt cccgatctcg ccgaccgagg
      301 cgccggaggt gtcgtacggc tttcacgagt accgtgtgtg ggcgacgttc cgcgagccgc
      361 gtgcgttcaa cacgcaggcc gaggtgctct tctcggccat catcgagcag gcgattcgat
      421 cgagcttcga cttcaaccgc cgcgagatcc gggccgaggc gggcctgcgc ctctcgcccg
      481 tgtacaccgt gtcgggcctc tattcgttcc agaataccaa gctgttcaac gtgaacatcg
      541 cgcccgagga cgagccggtc atcgaccgcc tgttcccgcg ggtgcgcatc gcgaaggtgt
      601 cagggtcgat catccgcgac agccgggacg acccgctcga tgcgtcgcgc gggtcgctgt
      661 tcatcgtcaa cgccgacctg gcggcccgcg cgctcggatc ggaggtcggc ttcatcaaga
      721 ccttcgcgca gggcttcttc tatcgccagc tgccgtcgcc gcgccggctg gtgctcgcgc
      781 tcggcgccag ggtgggagcg gcgtacgggt tccttcggac ggtcgaggga gagaccgtac
      841 aggacctgcc agccagcgag cgcttcttcg ccggcggcga cacgtccgtg cgcgggttct
      901 cgctcgatcg gctcggcaac gaacgcacga tcacgcccac cggtttcccc accgggggaa
      961 acagcgtcgt cgtgttgaac agcgaattgc gcttcaacct cacctcgaag cttcagacca
     1021 tcggcttcct cgacgccggc aacgtctttc cgagcgcgag cgacctgagc gtgatcgacg
     1081 tgcgtcccgc ggccggcttc ggcctcatgt atcgatcgcc gttcggcccc gtccgcgtcg
     1141 atctcggttt caacctcgac ccccaggagt tcgtccctgg agtcacggag cgggggaacg
     1201 tcctgcacat cctgttcgga gaggcatttt gagagcgagg gtgttgctgc tgtctcccgt
     1261 cctttgcctg ctgtgcggcg gagggcagct cgacgcgcaa cagctcctcg acaaagtgct
     1321 cgcgcgagtg ggtacgagag ccgtgacgct gacggaagtc agggcggcga tcggtctggg
     1381 tctggtagag gtcaggcgcg gcgaggacgc cgacgcggcc gggctgcagc gcgtgatcga
     1441 gcggcagctc gtgctcaacg aggtcggccg gtttccgaca ccggatccca ccgctgccga
     1501 gctcgccgaa caggtggctg cgatgaagtc gcacgccggt gcgtcatacg agcgcctggc
     1561 ggcgtcgacc ggtctggacg aggcaaagct gcaggaactg gcgagacaga cgctgcgtat
     1621 tcgcgcctac acgacgctgc ggttcgggac gacggcgcag gtgaccgagg aggaggcccg
     1681 gaagtacttc gacgaccatc cggagcagtt cacgcgagaa ggcgcgcgcc tctcgttcga
     1741 gcaggcggcg cccgccgcgc gccagcgggc atccgccgag cggcttcgcg ccaccaccga
     1801 cgaatgggtc cgcgaccttc gggcgagagc cgacgtcgtc atcgtcggga gtggacgata
     1861 gagtcgggca gaagagcggt cgtcaggaga ctccgatcgc gacgcgaagc acctcgatcg
     1921 aattgaaccc tgcatggctg aggatcggcg cgatgctgct gcgccgccac agatagagga
     1981 gggcccagaa cgcgccgagg aggcccgtcg tcactgccgc gtcccagccc tgcatcacgt
     2041 ggcccagccc gaacgcggtg ctgaggacca cgaccccgac cgccgccccg cccaggtgct
     2101 gttcgaaccg ccgcaggaga aacgcccgct gaagctcctc tcgcagacca ccggcgacga
     2161 tcgcgatcac gcccatcatt gcggcatctc cgtatcctcc ggcggcaagc gtttcgaagg
     2221 gattcatcgg cacgttgtgg agcgaaggga agaactggag gagcgacgtc atcagcaggc
     2281 ccgcggcgac gacggtgagc gggacgaggg ccagcccgaa gctcacttcg ggcacgaggc
     2341 ggtgccgccc ccgccacagc agccacgggc tttcgccgtg cgcccgcatg aacaccacca
     2401 tcagtgcgat caggagggcg ctgtcggcaa gcgacagtgc gaagacgaac gggagcgaaa
     2461 gctggtcatc cggcgttcgg ggcgccaggc ccagagcccc gagcaggccg gcgatgagaa
     2521 cctgcgtcgg cacgctcgag cacatcagga cctcgatgac cgcgacaacc cgctgctggc
     2581 tgcggctcag ccccggcccg ggagttgctg gcagaggttc gctcgacacg agcggctagt
     2641 ctagctggta gctggtcccc cagagctggg acccgctacg ggccaccagc ctcggctaca
     2701 ggcttaccgg ctaccggctg gctaaccgtg taagaatgac ctatcggcaa tgtcaacgat
     2761 acgccatgcc tcaaacaaac agtgaccgcg ttctcatcgt ggaggacgag cccagcacga
     2821 gagtcggcct gaccgagctc gtgcgtacgt ggggatttac gaccgattcg gccagcgacg
     2881 gggaagaggc cctgcagcgg atcaccgcct tccgcccctc gatcatcatc agcgacctcg
     2941 tcatgccgcg ttgcgacggt atcgagctgc tgcgcgcgct caaggaggac ggcggggact
     3001 acaccgtcgt aatcctcacg gcgcagggca cggtcgagac ggcggtggag gcgatgaagg
     3061 agggcgccta cgactacctg acgaagccga tagagcccca gcgcctgaag atcgtgctcg
     3121 acaagatcgt cgagcggcag gacacgctgc gcgaggtgaa agtgcttcgc aagcagctgc
     3181 gggaccacgg cacctacggc aagatgatcg gcagcagccc gcagatgcgc aaggtctatc
     3241 aggtcatcga gcaggcggcg ccgacctcgg cttcggtgct catctccggg gagtcgggca
     3301 ccggcaagga gctggtggcg cagaccattc atcagctcag cccgcgcgcc cagatgccgt
     3361 ttgtccccat caactgcgcc gccattcccg aaacgctgct cgagagcgag atcttcggac
     3421 acgagaaggg ctcctttacc ggcgccgttg atcgacgcgc cggctgcttc gagctggccg
     3481 atcgggggac gctcttcctg gacgagatcg ccgagatgac gccgtcaacg caggtgaagc
     3541 tgctgcgcgt gctccaggag cggaagttcc ggcggctggg cgggcgcaac gaggagaccg
     3601 tggacgtccg cgtcatcgcc gcgaccaacc agaatcccgg cgatgccgtc aagaacggca
     3661 agctccgcga ggacctctac tatcggctga acgtgttcgc catcgagctg ccgccgctgc
     3721 gtctgcggaa ggaagatctg ccgctgctgg tgcagtcgtt cctcggcgat ttcaactcgc
     3781 ggaacggcaa gaaggtgtcg gcgctcgatc aggcggccat gcggatcctc gagcaataca
     3841 actggccggg caacatccgc gagctgcgga acgtgatcga gcgagcggtg atcctgagcg
     3901 cgggcgagtt catcgatccc aagcttcttc cgccgctggt caccgaatcg cccgacgtgg
     3961 tcaagccgac gctctcgctg acgccgggca cgactgttga agaagcggag acgaggctga
     4021 tcctcatgac gctcgagcat acgcgcgaca acaagacgcg tgcggccgag attcttggca
     4081 tcagcctgaa gacgcttcac aacaagctca acaagctccg cggccgcatc ccgggaaagc
     4141 gcagcgaaga ggaatcggtg ccgcagtaac acaacatgcg actgagcatc cgggtcaagc
     4201 agatcgccgg agtgaccgcc atcgtcggcc tggccgtcgt ggcgctgagc gcgttctaca
     4261 cggcccggct tgccaacgtc gtgctcgaag aaagtcactc acgggggcag atgctcgcca
     4321 acacgatcct gcatcgcgcc ggcagcatcg tcaccggcga cgtcgatccc tatgcagcgc
     4381 tgcgtgacga cccgggcctg cggtcgatcc tcgagtcgag catctacgga gaatccatca
     4441 gctacgcggc aatcggggac accgacggcc gcgcgatcgt tcacagcatc acgagcatgg
     4501 tcggcaacac gctcccggtg cggaccgatc tggtaacgat cgtcgccgac tcgccggtgg
     4561 agaagatgcg ggtgatcttc gctggagatg gccagacgct ggaggtgcgg gtgccgctca
     4621 ctcggggcgg cgaggctttc ggctcgattc gcatcggcgt atccacgttg tttttgcgcg
     4681 gtcacctcag gaagctgctc accgatgcgg ggttcacggc gctcggtgcg ctggccgtgg
     4741 cgatcttcgt ggccggcgtg ttgtcgcagc ttctgctgag gccgattcac gtgctgcgcg
     4801 gcggactgac gcgcctgggc aagggcgagt tcggcgtgac gctcgacatg ccgtccggcg
     4861 acgagttcgg cgagctggga gacttcttca acacggtcag ccagcagctg tccgcggatc
     4921 gaacgctgct ggcaggacag aaggccaacc tgcagtcggc cgtcgagcac ctcgaggacg
     4981 ccgtggcgct cttcaacccg tcgggcgagc tgctgttcag caatcccgcg atgcagcaca
     5041 cgctcccggg cgacgccatc ggccgcgtgc tggatgggct gctcccgacc ggccatccgt
     5101 accgcacgat cgtcgaggag acactggcga cgcgaaaatc ccgcggcccc gtgcagattc
     5161 cgggtcacag caccgctcag atcgtcgtgc ctggacggca gccgccggag accgctggcg
     5221 cgcaggcgct cgccggcacg acggcgcggg atggccggcc cgccgacgag gagctcatcc
     5281 tgacgaacgc gatcgcgggc acgcacggcg agctcgtcgg tgtgctgctc gtgtcccgca
     5341 atctggcgag cttgtcgcgc atgcagtcca cgctggccta ttcacgcaag ctcgtcgcgc
     5401 tcggccggct gacggccggc atcgcgcacg aggtgaagaa cccgttgaac gcgatgatga
     5461 ttcacctgga gctgctgcgc acgaagatcc gcggcacctt cgtcccggcg atgcaacctg
     5521 agccggtggc ggccggcggc acgctgggtc tcgggccggc cagggcagca ccactgccgc
     5581 ctcccgtcca gggcgcgctc gagcacgtcg aaatcatcga gagcgaaatc aggcggctgg
     5641 atgaggtcgt gcagggattc ctgaagttca cgcgaccgga agacctgagg ctccagcccg
     5701 tgcgggcggc ggcgctcttc gaagagatcg cgccggtcat cgagaccgag gccggcaagc
     5761 acggcgtgaa ggtggtcatc gacgtgtcgg ccgggacgcc caacgtgaac ggcgatgcct
     5821 ccatgctgcg ccaggtcttc ctcaacctgg cgatcaacgc gtgtcaggcg atgccgagcg
     5881 gcgggacgtt gcgtctggtg gccgcggccg cgtcgcgcaa ccgggtcgag gtgcgcgtcg
     5941 aagacaccgg cgtcggcatc aagcccgagc acttgagccg gattttcgac ctgtatttca
     6001 cgaccaagga gcgcggcacc ggtatcggac tctcgatggt ctaccgtatc atccagttgc
     6061 acgacggcga gatcgaggtt caatccacgc caggccgtgg cacgacgttt cgcgtgttgc
     6121 tgccgcgcgc gcaggggatg tgagatggca gtcagttccc ggctctccgg ttgggcggcg
     6181 gttctcctgg gcgtcgcgat gacggcttcg ctctcggccg ggtgtgcgaa ggcgcgcgcc
     6241 gcgaccgtac cggacggtcc gccgctcgcg atgccgctgc cgccgccgcg cgtgttctcg
     6301 ccgctagaag aggagccgct ggccgcctcg cccgccgtcg ccgagccggc ggcgtcggga
     6361 gcgccccagg tgacgcagaa gcaggaccga gctgcgaacc ggccgtctcg cgagcccgag
     6421 aaggccgaag ccccgccccc accggcgccc gctccggtcg tggagcccgc gcgggagctt
     6481 cgtgccgcgt cgacgccggc cgacgccgaa gccgaccgga agatccgcct tctgctcaat
     6541 gccgccaagg gtaatctgga gaaggtcaac tacggcaagc tctcgacggc gggcaaggag
     6601 cagttcgacc aggcgaggaa cttcgcgaca cagggcgacg aggcgctgac gcagcgaaac
     6661 tacgtgttcg ccgagacgct cgccgacagg gcggcaaagc tcgccacgga gctactgggt
     6721 ctgagtcgct aatccgccac aaggatcacg acgctcgtgg ccgcgacctt cgtgtgttcg
     6781 tggccagcga agcggttcag aaggtgatgc cgaatccgaa gtgcactctg gggtttccgc
     6841 tctcgggcca cgcgacgtcg aggtccagga tgagcacggc gatgccgaag tagacgccgc
     6901 cgccgatacc gcgctcgaac cgctgctcct tcaagcctgg tccatctgac gcccatgtcg
     6961 tgccggcgtc cacgaaggcc ttcacgccaa atcggccgac gttgagcggc gaggtgagcg
     7021 gataccgcac ttcagccgag aaggcggaca ggttgtcgtt ggcggcgtac cccgcgcggt
     7081 agccgcgcag cgactccttc ccgccgagca gcacctgttc gggcgcgggc agcgggtgat
     7141 tgctcgtggc gacctgcgcg cgcagcgcga gcaccgccga tccgcccacg ccgacgtatc
     7201 cgcgggcatc ggcgagccac cgcccggcgg agccgtcggg aaacgccagc cgctcccacc
     7261 ccacccggac gtgaatcgcg ttccgcggaa aggacggatc gatgcgcgta tccagcgtga
     7321 cgtgcacgcc gccggcccag tgacgcgcgt cgtagatccc gccgaagtcg acgtgctcga
     7381 cgcggccgtc ggcgcccacc cggagccacg gatgcagaga gcgttcgacc gcgacgcgcg
     7441 cgccctgccg caggtcgctc gcgtcgtagt gcgggttcac ccgacggtcg atcgacaggg
     7501 cgccgctcac gagcgagaag gggccgcgat cgaacgagcg ctcgacctcc agggccgcgc
     7561 ggcgctcgcc gccccacgtc agcggaatcg acaggcggct gcgatcgccg aggctgttcg
     7621 cgaagccgaa ctgcacgccg taggtcagtc cgtatccgtc ctcgtagttg aggaccggca
     7681 gccactgttc cgaggcgaag aagcgcttga tcgggctggg cgtcagatcg tcggggctga
     7741 ccgccgcatg
//
LOCUS       DBNGSH010000441        19238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2757533, whole genome shotgun sequence.
ACCESSION   DBNGSH010000441 DBNGSH010000000
VERSION     DBNGSH010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 19238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19238
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2757533"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="AC16CY_10080"
     CDS             <1..646
                     /locus_tag="AC16CY_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354179.1"
                     /translation="SYLVTYAVFRLFFNYGFMAEAPEYVASAPAGMYNAVSALVFYVT
                     ALAVMFLVLCFDLWPMTTVAGVMKQPVLGMVWTVVTLAGAAVAMWIGVERQGIDPMSF
                     LTGVSAPFIFGSIIVLNMLQNSLFAKIPQPLKGVMNSIAAAAIGLVLAQLYVSLSPTV
                     TGRLASGPPGYEREIWLANALLSVTFPFLIFHAAFFGYWPIPIRGSDGKHPGKA"
     gene            804..1436
                     /locus_tag="AC16CY_10085"
     CDS             804..1436
                     /locus_tag="AC16CY_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3354180.1"
                     /translation="MRIRRAMVLLPLLLVAPATTYAGPIVAGDTVVLTDGPGTTGGGE
                     FNLFVNGSTTSFVTFCLQRTEFISYNTPFRVGSVTNYADDAAGNDPIESQTAWLYTQL
                     RLNPNSIGYQHNQTEANAFQTAIWYFENEITLTNAQKLANKYIVGANQAVANGYSGIG
                     NVRVVNLFYMNGKAAQDQLILQVAEPATLTMFASGLLAFGAFRRRFSTRA"
     gene            complement(1442..2104)
                     /locus_tag="AC16CY_10090"
     CDS             complement(1442..2104)
                     /locus_tag="AC16CY_10090"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HIR3354181.1"
                     /translation="MTEQATGIRPQASEALALIAEALAAAEKPCLTSSFQAECVVLTH
                     MLREVRPDIPVLFLDTFHHFPQTLAYRDEMTARWGLNLVTLKAASPRVGLWNVESTDA
                     CCRRHKVEPLFSALEAYDTWFTALRRDQSPSRAHLKETEPFKLPGRTINRISPLAGWT
                     MRDVWKYAKDHDIPLLPLYEAGFTSIGCEPCTAVPFDPENPRSGRWQGQKLECGIHIQ
                     VS"
     gene            complement(2101..3444)
                     /locus_tag="AC16CY_10095"
     CDS             complement(2101..3444)
                     /locus_tag="AC16CY_10095"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HIR3354182.1"
                     /translation="MEAGLLRICTAGSVDDGKSTLIGRLLYDSQSVYEDQVSSVEKAS
                     KNRNAGPIDFSLFTDGLRAEREQGITIDVAYRYFATARRKFILADTPGHEQYTRNMAT
                     GASTADVAILLIDARNGVRTQSKRHARIARLLGINDFVLAINKMDLVDFDQDVYERIQ
                     DEFSEFLAGATVHAIPLSALHGDNVITRSSRTPYFDGAPLLEFLETVRVHRNPAAAPF
                     RFPIQMVVRPDDEFRGYAGQIVSGTIKPGDRVAAWPSGRSARVKRVVTYDGDLALAFA
                     PMSVTLTLDEEIDISRGDVLSSPQEAPGQEGSRSSHPLVGQTFEAEVVWMDERPLDPG
                     RVYILKHGTRTVTTEINHGLVLNQIGVVQVSTARPLVFDRYGDSRSTGSFILIDPATQ
                     FTCGAGMIREPVREASPVHSAPLSFAERLAHLARRAASDDEAAEAVRKALEEMLT"
     gene            complement(3444..4385)
                     /gene="cysD"
                     /locus_tag="AC16CY_10100"
     CDS             complement(3444..4385)
                     /gene="cysD"
                     /locus_tag="AC16CY_10100"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR3354183.1"
                     /translation="MPTMTTSPDPTLATLRLDHLRALEAESIHILREVVAEFERPVML
                     YSIGKDSSVLLRLAQKAFYPGPIPFPLLHVDTTYKFREMIQFRDDMARAVGARLIVHT
                     NEEAIAEGTQPFKVGTQRCCGLLKTKSLLDALQAGTFDAAIGGARRDEERSRAKERIF
                     SVRDPNGQWDPKRQRPELWTLLNAKLRPGESIRVFPLSNWTEIDVWHYIHEERIPIVP
                     LYFAKEREVIVRGNSLIVQEQPFVVTMPGEKPQTVKCRMRSLGCSPCTGAIRSDADTV
                     PKIIDELIATRQSERQLRIIDHDQDGSMEVKKREGYF"
     gene            complement(4651..5568)
                     /locus_tag="AC16CY_10105"
     CDS             complement(4651..5568)
                     /locus_tag="AC16CY_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3354184.1"
                     /translation="MDSLQLALALITFGAAIVNGALGYGFSSITVPIALLFLTNRVLN
                     PALVPIEVVLNAYVLWVNRDALPTVWRRVLPIVLGLVPGVVLGTALVSQVNPGWLKFG
                     TFIALLPLILVQAAGYRRPIRSERSVGLVFGGGVGVLYSVTTISGPPLAVMLSNQGLT
                     KRDFRAALGFIRLAESSFTAVAYAYAGLYTAQSMALIPYIVPSILIGVPIGSYLIQRI
                     RPETFRRVCMSFDAWIVGFGLSALLKDLGLVESNLAYLVLAGVALLDTWLLYRFFAVQ
                     LPMVKRTEALVAAPPGPSPPAGAPPPGAF"
     gene            5622..6080
                     /locus_tag="AC16CY_10110"
     CDS             5622..6080
                     /locus_tag="AC16CY_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3354185.1"
                     /translation="MLTKKSKYGLKALLMLAEEPDRRPVLIADLADRQRLPRKFLEAI
                     LLELKRRGILQSKKGKGGGYFLGRDPAHVTLGEVIRVLDGPLALIPCVSQTAYMKCEE
                     CLDEQTCGIRLAMKDVRDATARILDNTSLVALNTQVARARRRARSSRRSS"
     gene            6203..8794
                     /locus_tag="AC16CY_10115"
     CDS             6203..8794
                     /locus_tag="AC16CY_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3354186.1"
                     /translation="MSPRSLPAVVVLFLALLPAAVSGQAGTGTIAGIVKDSTNAPLPG
                     VTVTVVGDALGATLEAVSDERGAYRADALAPGAYRVQTTLDGFEPSVIRVVLEAGQTA
                     AVDVTLTPARLTENVVVTARRVEEAIQEVPIPVSVLSGELVESAGAFNVNRVKELIPT
                     VQFYSSNPRNSAVTIRGIGSPFGLTNDGIEPGVGLYIDGVFYARPAAATLDFLDVAQV
                     EVLRGPQGTLFGKNTTAGAITVTSRRPTFTPDANVEVAYGSYDFIQTKASVSGPFGNK
                     VAARASFSGTGREGFVHNTATRTDTNTLNNLGFRGQALIAPSRSVAINVSVDHTRQRP
                     EGYAQVVAGVAPTLRPANRQYAQIAADLGYAPPSFNAFDRLTDTDTSWQSDQDLGGVS
                     GTVDWSAGRGRVTSVTAYRYWHWDPSNDRDFIGLPVTSVSAAPSNQSQWTQEVRYAGA
                     LSPSLNLVTGVFAFKQALDSNPSFRQEQGSAAARFLLAPTPAAATPGLLDGYGFDQDL
                     RYRNLSAAAFGQLEWLVTDRLRVLPGLRFNYDQKDVDFNQTVYGGLQTTDPVLVALKL
                     SVLAPQAYTADVDDTNTSGQLTVAYKVSPSVNAYGTYSTGFKSVGLNLNGVPTDALGN
                     PILSAATVKPEDARHVEFGVKTEFLPGVTANFTVFNTAIKDYQTQVVNSQVGVLRGYL
                     ANAEKVRVRGAEFDGSARINPRLSFYGAVAYTDGIYVSFPDAPPPLEDTGGLPVKDIS
                     GSVLPGISKWALTFGGEYNKPATVFRQAGEFFGAVDTSYRSEFSSSPSASRYLVVDGY
                     GLVNLRAGFRWSDGWSLFLWSRNLLDEQYFEFLTAQPGNSGLYVGLPGDPRTIGLTLR
                     MKLGNGQ"
     gene            8869..9372
                     /locus_tag="AC16CY_10120"
     CDS             8869..9372
                     /locus_tag="AC16CY_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354187.1"
                     /translation="MDPLFAWIETTALSTFLRESPSLWVFPFVLILHTVGLAFFVGAN
                     VAWDVRLLGFSTGIPVETLRRYFLVMWIGLVINGVSGVLLLIAYPTKALTNPLFYVKL
                     VLIGAGIWAALRLRHDVQGRGSGGDALPTRIRALAGASLACWVAVIFAGRFLAYTCTR
                     LTVDTIC"
     gene            9366..9863
                     /locus_tag="AC16CY_10125"
     CDS             9366..9863
                     /locus_tag="AC16CY_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354188.1"
                     /translation="MLDETLRSIQMFLYPMPQGELLRETRWAWPIFESLHFLGMSLLL
                     GTVGIFDLRLLGFARRIPVAALHKLIPIGIAGFAMNALTGISFLSATPDQYLFNRAFY
                     WKVAFFVVAGVNVMVFYARTFRRLLDTEPGAPPPLGARIAGGVSLIAWIGVMSAGRLL
                     TFFRP"
     gene            complement(9876..11450)
                     /locus_tag="AC16CY_10130"
     CDS             complement(9876..11450)
                     /locus_tag="AC16CY_10130"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3354189.1"
                     /translation="MPSEHRLHPSSMLFSFAGTLKTFALPALVVYFTAGRSQEPAPFV
                     GRWMPWADISNGEFWMLLLLVPGAIAAVARYLSFRVRYEGTELVIRSGILFRNERHVP
                     YLRIQNLDTSRNVVHRLLGVTNVRVETGGGNEPEATISVLDDRVFDEMRRRVFEGRAR
                     AGGPREATADEAPVAAPDEALTLLHLPLRELLLHGFLENRGLILIGAAYGALWQFGLP
                     TALWQRLTGGWDAPRLVNEALQEVESGHAVTIVQVAILIAGLVGILVLVRLVSMAWAV
                     TRLYDFRLSLAGQDLRAEYGLLTRVSTRIPLPRVQTLTIRETPLQRLVGRMSVRAETA
                     GGHGPAEGGARPFERERLAPIVETDRVPALVRQVLPGFDIGALAWQPIHPRAFRRALK
                     PYLFVALAPVVPLVFFLSVWWLAATPITIAWAVVASRKHVEHMAWAITDTAVVFRSGW
                     LWRHVTVAPIAKIQAVACSESPFDRRAAMRSVRVDTAGGNERSHRVDIPYLGRDTADV
                     LHLRLAEQISRTAFRW"
     gene            complement(11440..11961)
                     /locus_tag="AC16CY_10135"
     CDS             complement(11440..11961)
                     /locus_tag="AC16CY_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3354190.1"
                     /translation="MDSDSDAVESIADGVDRKLDPRVIPLQRTLGWIPTAILSAISLV
                     FVTFVWADSGILALGLLLMPLWLAGVVALAWQFQRWPAIAYRFASYRIDELGIEIRRG
                     VYWRTITNVPRSRVQHTDVSQGPLERRYGLGTLVIYTAGMAHSRVNLEGLDHEVARRI
                     RAHLLPKDEGDAV"
     gene            complement(12008..12862)
                     /locus_tag="AC16CY_10140"
     CDS             complement(12008..12862)
                     /locus_tag="AC16CY_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354191.1"
                     /translation="MSIAIASRVAAACVFAAATTASPSAQWPAYPTPNVPRTADGRPN
                     LLAPAPRASDGKPDLSGIWSNPGWREIGAGTGVSGTGGAPGTPSVLPRGPGLFFNIAS
                     GVAGGLPLQPWAAELLKQRMAGNSKDNPDAHCLPMGNMQLYTHPQPRKIIQTPALIVI
                     LYEGNAGIRQIFTDGRPLPRNDPQPWWFGYSTGRWMGDTLVVQTSGFRDGGWLDVNGS
                     PLTDAATMTERFTRVNYGALDIEVTVDDPKAYTKPWTVTIRNEVMLDTELIEFVCLEN
                     EQSTKHFR"
     gene            complement(12933..14114)
                     /locus_tag="AC16CY_10145"
     CDS             complement(12933..14114)
                     /locus_tag="AC16CY_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3354192.1"
                     /translation="MAIETGRAGAAVGVMLIASLAACDGSEPGLQSNSSTPPTTATTQ
                     TYNMRLMARVDLNALMSAPPLHALHHDEPIEGLGALSGSGNWGYTAPNGRRFALTGTS
                     AGLSIVDVTRPDQPRNITLIAGPASQWREVKTWGPYAYVTTEAPHGLDIVDLRNPDHP
                     EKLRTWSDTFFSAHTLCVDDARGLLFVNGTADHNGERIGMRVLDVQRDPADPREAGSF
                     TDFYIHDCYVRGNYLFASAIYDGFVAVLDISNPARITELTRFFTGGRFTHNSWLTADG
                     RYLFTTDERAGRPLEGWDLADMRNPRKVSEYIAQPGTIPHNVMIDGSRMVVSHYTEGV
                     HLLDVSNPAQLRVLGFFDTYEGSSSQFAGNWGAYIFPSSDLIVASDLQGGLFVIQYTG
                     N"
     gene            14216..14479
                     /locus_tag="AC16CY_10150"
     CDS             14216..14479
                     /locus_tag="AC16CY_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3354193.1"
                     /translation="MAYIICEPCVGTKDSACVDVCPVDCIHPRKDEPGFESAEMLYIH
                     PDECIDCGACVPACPVEAIFALDETPQKWANYIPMNAAFYQKS"
     gene            14542..16056
                     /locus_tag="AC16CY_10155"
     CDS             14542..16056
                     /locus_tag="AC16CY_10155"
                     /inference="COORDINATES: protein motif:HMM:NF025341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HIR3354194.1"
                     /translation="MSSSLSRPAQALTCALSLLVGSSLSLRAGQDADASLQLHLATLL
                     YDETRYLEALDAFTDATKTTDPRLGVAARKGTVRTSLKVAEFKRAREEAETLRGLAPD
                     DPEALTLHADALWSMGLFDEAEREYRDALARTPGSARARYGLARSLAASSRLSDALDS
                     ALAASAAAPRDGEIHALIGDIYERLHQFDQAANAYRNYINLLPNKDRSEKAAWAKAQV
                     SFLDSFSGVVPVEIDRLDLETLHTLPFRLVDDKIIVQARVNGGQSREFILDTGSEETT
                     LSRETAQREGVRGVTFTLSAGVGEIGLRGLQLAQAESLDLGTLQIRNLPVLIKNPALR
                     GIPKREGNSFSPLSMGMSLRIDYERRLLTIGKRLPPVEADIRLPMRINRLAMVRGLVN
                     STHPAYFVVDTGGQVISISAETASALAPSQFRRIPLKVWGTSGWDRDAFLLPGVDLDF
                     DEIEYRNFSLVVLNLRAPSILLGFQLGGIVGHNFLAPFRVTMDLENSELLLERL"
     gene            complement(16155..>19238)
                     /locus_tag="AC16CY_10160"
     CDS             complement(16155..>19238)
                     /locus_tag="AC16CY_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354195.1"
                     /translation="TLLRVVSAAAALAYAIPATAQTPYVPYYGKNQIRYDNFEWYHYE
                     TDHFIMYYYPEIEPHLERMASYAESAYQHVSSELKHDLAHKVPLILFQTSSEFQQQNV
                     IPGAAQEGVGAFAEPTRQRIVMPMDEPPDLLYRLIVHELTHQFDYDIIPASLVRRTTP
                     LWLHEGLADYMTGFWRPLDLMSVRDAAVADIIPKMTRLEGYSDVGGPRMIYNLGHAIF
                     EFIEARWGKEGIRQYLFSLRKSVIGGGEDAYEETFKITADEFDSEFDKYLKEKFKPFR
                     DKERPADYGRNLVPNPERSPYRGAYTVEPSPSGDLLAIFTGNARDQEGDIVIISARDG
                     SEFRNLTQGFDYKLGFEYIATSGNRWVTVPQLSWSSDDTLGYFARSEKSRSLILQNVI
                     TREIVKRIDMKTVDDPESPDFSPDGRRVAFAALQGGTGDIFVVDIQTEEVTNITKDGF
                     ADSGPTWAPDGRSIVYLARISGNEKLFRVDLATGEKTQLTFGTHDDATAHFLDDNTLV
                     FPSTATDPAQPIDPDVARNGQIYNVWMLDLKTGELRQYTDAVGGNLYTAVLKGDTATP
                     RLIVVTYHKGEYELHLIDRRDPIVTAASSDFGAPGPIIDFQAPLSHTIVASNKKKKGK
                     FENMFLDGRPPVSVGVTSSGDVFGGTAVTFSDVLGDQQFSVYVASISQYRTMSFAYTN
                     LASRFNYMLQAYSQTEFFYGNMAGVFYDPVYSGLVDRDLAIATRSVRGGSVYGIWPLS
                     RYRRLEVTAGVANYNESFNDPVLDEYSEQYQQDNYGRQLFRSGTFVPMGVNFIQETTV
                     FREFGPVSGSTMRLSYELAPPIGNTLTRQTFDVDARYYQRLASTGVLALRARAFRSRG
                     DSPDFMYFGGNSEMRGYEYLQFAGDTAAFFNAELRFPLINAMLTPIGVLGGVRGVFYA
                     NFGGGYFNGQPFTWAKNDSQLYAPVVGFVPGPGLGQQTPVYGVPQVISGLRLVDSRAS
                     YGMGIQTMALGFPVHLDWSWKTLFNREWEDALFRPYGGSEEFRRVQFKIWIGYDF"
BASE COUNT         3248 a         6241 c         6327 g         3422 t
ORIGIN      
        1 gtcttatctg gtcacgtacg ccgtgttccg gctcttcttc aactacggtt tcatggcgga
       61 ggcgccggag tacgtggcct cggctccagc cgggatgtac aacgccgtgt cggccctcgt
      121 cttctacgtt acggccctcg cggtcatgtt cctcgtgctg tgcttcgact tgtggccgat
      181 gacgacggtt gcgggcgtca tgaaacagcc ggtgctcggc atggtctgga ccgtcgtcac
      241 gctggccggg gcggccgtcg cgatgtggat tggcgtcgag aggcagggca tcgatccgat
      301 gtcgttcctc accggcgtga gtgctccctt catttttggc tcgatcatcg tcctgaacat
      361 gctgcagaac tcactgttcg cgaagatacc gcagccgctg aagggcgtga tgaatagcat
      421 tgccgccgcc gccatcggcc tcgtgctggc gcaactgtac gtgtcgcttt ccccaacggt
      481 gaccgggcgg ctggcctccg gcccgcccgg ctatgagcgg gagatctggc tggccaacgc
      541 gctcctgagc gtgaccttcc cgttcctgat cttccacgcc gcgttttttg gctattggcc
      601 tattcccatt cggggttccg acggcaagca tcccggcaag gcatgagtgg aaggccgcca
      661 ggccgagtac aaaccgcgcc gcttggacaa acgtctgccc gcccgggtat ttagcgctga
      721 gcttggccgg ctaaagcttt caaaacagca agttacagct tggcccaggt cttgtaacgg
      781 ttgaggtcga gggaaactgc tcaatgcgca tccgtcgggc catggttctt ctgccgttgc
      841 tccttgtcgc tcccgcaacc acgtacgccg ggccgattgt ggctggcgac accgtggtgt
      901 tgactgacgg ccccggcacg accggtggcg gcgagttcaa cctcttcgtg aacggcagca
      961 cgacgtcgtt cgtcaccttc tgcctgcaga ggaccgagtt catcagctac aacacgccct
     1021 tccgcgtggg cagcgttacc aactatgccg acgacgctgc cggcaacgac ccgatcgagt
     1081 cgcagaccgc gtggctgtat acgcagttgc gcctgaatcc caattcgatc ggctaccagc
     1141 acaaccagac ggaggcgaat gccttccaga cggcgatctg gtatttcgag aacgagatca
     1201 ccctgacgaa cgcgcagaag ctggccaaca agtacatcgt gggggcgaac caggccgtgg
     1261 ccaacggata ctccggcatc ggcaacgtgc gcgttgtcaa cttgttctac atgaacggga
     1321 aagcggccca ggaccagctg attctccagg tggcggaacc agcaacactg acaatgttcg
     1381 cgtcaggcct gctggccttt ggagcgttcc ggcgtcgctt ctcgacgcgc gcctaggtct
     1441 tctaactcac ctgaatgtgg atcccgcatt cgagcttctg gccctgccag cggcccgagc
     1501 gggggttctc tggatcgaag ggcacggcgg tgcagggctc gcatccgatg ctcgtaaagc
     1561 cggcctcgta cagcggcagc agcgggatgt cgtggtcctt cgcgtacttc cacacgtcgc
     1621 gcatcgtcca ccccgcgagt ggcgagatcc ggttgatcgt tcggccagga agcttgaacg
     1681 gctcggtttc cttcagatgg gcgcgcgacg gggactggtc ccggcgaagg gccgtaaacc
     1741 acgtatcgta cgcctcgagc gccgaaaaga gcggctccac cttgtgccgc cggcagcagg
     1801 cgtcggtgct ctcgacgttc cacagcccga ctctgggact cgccgccttg agcgtgacga
     1861 gattgagccc ccaacgtgcc gtcatctcgt cgcgataggc gagcgtctgc ggaaagtgat
     1921 ggaacgtgtc gaggaagaga acggggatgt cgggccgcac ctcccggagc atgtgtgtga
     1981 gaaccacgca ctcagcctga aagctcgagg tcaggcaagg cttctccgca gcagccaatg
     2041 cctcggcgat cagcgccagt gcttctgagg cctgaggcct gattcctgtg gcctgctctg
     2101 tcatgtaagc atctcctcga gggcctttct gacggcttcg gcggcctcgt cgtcggacgc
     2161 cgcccggcga gccaggtgcg ccagccgctc ggcgaagctg agaggcgcgc tgtgcaccgg
     2221 cgaggcctcg cgtaccggtt cgcggatcat ccctgccccg caggtgaact gcgtcgccgg
     2281 atcgatcagg atgaagctcc ccgtgcttcg gctgtccccg tatcgatcga acacgagggg
     2341 cctggcggtg gagacctgga cgacgccaat ctgattcagg acgagcccgt ggttgatctc
     2401 cgtcgtcacg gtgcgcgtgc cgtgcttcag gatgtagaca cggccggggt cgagtggccg
     2461 ttcgtccatc cagaccacct ccgcctcgaa cgtctgcccg accagcgggt ggctgctgcg
     2521 ggaaccctcc tgcccgggtg cctcctgcgg cgagctcagc acgtcaccgc ggctgatgtc
     2581 gatctcctcg tccagggtga gcgtgacgga catgggtgcg aaggcgagcg cgaggtcgcc
     2641 gtcgtaggtg acgactctct tcactcgggc gctgcggccg gacggccagg cggcgacgcg
     2701 atcgccgggc ttgatcgtcc ctgacacgat ctgtcccgcg tagccccgga actcgtcgtc
     2761 gggccgtacg accatctgga tcgggaagcg aaacggcgcg gcggccgggt tgcggtgcac
     2821 ccgcacggtt tcgaggaatt cgagcagcgg cgcgccgtcg aagtacggcg tacggctgct
     2881 gcgggtaatc acgttgtcgc cgtgcagcgc gctgagcggg atggcgtgca ccgtcgcgcc
     2941 cgcgaggaac tcggagaact cgtcctgaat cctttcgtat acgtcctggt cgaagtccac
     3001 caggtccatc ttgttgatcg cgaggacgaa gtcgttgatg ccgagcagcc gggcgatccg
     3061 cgcgtggcgc ttcgactgcg tccggactcc gttgcgcgcg tcgatgagga gaatggccac
     3121 gtccgccgtt gacgcgcccg tggccatgtt gcgagtgtac tgctcgtgtc cgggcgtgtc
     3181 tgccaggatg aacttgcgcc gcgcggtcgc gaagtaccgg tacgcgacgt cgatcgtgat
     3241 cccctgttcg cgctcggccc tgagcccgtc cgtgaagagc gagaaatcga tcggcccggc
     3301 gttgcggttc ttcgacgcct tctcgaccga cgagacctga tcctcgtaga cggactgcga
     3361 gtcgtacagc agccgcccga tcagtgtgct cttgccgtcg tccacgctcc ctgccgtgca
     3421 gatgcgaagc agtccggctt ccatcagaag tagccttccc gcttcttgac ctccatcgag
     3481 ccgtcctggt cgtggtcgat gatgcggagc tgacgttcgg actgccgcgt cgcaatgagc
     3541 tcgtcgatga tcttgggcac ggtatccgcg tcggatcgga tggccccggt gcagggcgag
     3601 cagcccagcg atcgcatgcg gcatttcacc gtctggggct tctcgccggg catcgtgacg
     3661 acgaagggct gctcctgcac gatcagggag ttgccccgaa cgatgacctc gcgctctttg
     3721 gcgaaataca gcggaacgat cgggatgcgc tcctcgtgga tgtagtgcca gacgtcgatt
     3781 tcggtccagt tcgagagggg gaacacgcgg atgctctccc ccggcctgag cttggcgttg
     3841 agcagcgtcc agagctcggg acgctggcgc ttcgggtccc actggccgtt cggatcgcgc
     3901 acggaaaaga tccgttcctt ggcccgcgat cgctcctcgt cgcgcctggc tcctccgatc
     3961 gcggcgtcga acgtgcctgc ctgcagcgcg tcgagcagcg acttggtctt gagcagcccg
     4021 cagcagcgct gcgtgccgac cttgaacggc tgcgtgccct cggcgatggc ttcctcgttg
     4081 gtgtggacga tcaggcgcgc gccgacggcc cgcgccatgt cgtcgcggaa ctggatcatc
     4141 tcgcggaact tgtaggtcgt gtcgacgtgc agcaggggga acgggatggg gcccggatag
     4201 aaggccttct gcgccagccg cagcaggacc gaggagtcct tgccaatcga gtagagcatg
     4261 acgggccgct cgaattccgc gacgacctcc cgaaggatgt gaatgctctc cgcttcgagc
     4321 gcgcgcaggt ggtcgagtcg cagcgtggcg agcgtcgggt ccggcgatgt ggtcatcgtt
     4381 ggcatgtctg gcgaaaccgg ccggcgggtc taaggacccg ccgctacata cgcccgtagg
     4441 ggcgggtctt cagacccgcc caaaaaaaaa gcccggggcg ttggccccgg gcttcgatcc
     4501 ctcagcgtgt tctgggttgt ttttttctgc tgtttacccc agtagccgga tcgtcaccgc
     4561 ggggaccaga aaggtcctca tgcaacaaca cacgcaggga cagcacccgg atgtcacgac
     4621 cctgaaattg taccctctga gcgccccaag tcaaaacgcg ccgggagggg gggcgccggc
     4681 aggcggggag gggccgggag gggccgccac gagggcctcg gtccgcttga ccatggggag
     4741 ctggaccgcg aagaaccggt acagcagcca cgtgtccagc agggccacgc cggccaggac
     4801 gagatacgcc aggttgctct cgaccaagcc aagatccttg agcagcgcag acagtccgaa
     4861 cccgacgatc caggcgtcga agctcataca gacacgcctg aaggtctccg gccggatgcg
     4921 ctgtatcagg taggagccga tcggtacccc gatcaggatg ctcgggacga tgtaggggat
     4981 gagggccatg ctttgtgcgg tgtacaggcc cgcatacgca tacgcgactg ccgtgaagct
     5041 ggactccgcc aggcggatga agccgagagc ggcccggaag tcgcgcttcg tcagcccctg
     5101 attgctcaac atcacggcca ggggcgggcc cgagatcgtg gtcacggaat agagcacgcc
     5161 cacgccgccg ccaaacacca ggccgacgga tcgctccgag cggatcggcc gccggtagcc
     5221 cgcggcctgg acgaggatca gcggcagaag ggcgatgaag gtgccgaatt tcagccagcc
     5281 cggattgacc tgcgacacga gtgccgttcc cagcacgacg ccgggcacga gaccgaggac
     5341 gatcgggagc acgcgcctcc agaccgtggg gagcgcgtcg cggttgaccc acagcacgta
     5401 ggcgttgagg acgacctcga ttggcacgag cgccgggttc agcacccggt tcgtcaggaa
     5461 caggagggca atcgggacgg tgatcgacga gaagccgtat cccagcgcgc cattgacgat
     5521 ggcggcgccg aaggtgatca gggcaagggc cagctgaagg gaatccatgg cgattcaggc
     5581 gtcgtgaata ataaccgaca aacactatag actgttaggt gatgctgacg aagaagtcca
     5641 agtacggtct caaggcgctg ctgatgctcg ccgaggaacc cgaccggcgc ccggtgctga
     5701 tcgccgacct ggccgacagg cagcggcttc cccggaagtt tctcgaggcc attctgctcg
     5761 agttgaagcg ccgcggcatt ctgcagagca agaaagggaa gggcggcggg tacttcctcg
     5821 gccgcgatcc cgcccacgtc acgctcggcg aggtcattcg cgtcctggac ggcccgctgg
     5881 cgttgattcc gtgcgtcagt cagacggcct acatgaagtg cgaggagtgc ctcgacgagc
     5941 agacgtgcgg catccggctg gccatgaagg acgtccgcga cgcgaccgcc cggattctgg
     6001 acaacacgag cctcgtggcg ctcaacaccc aggtggcccg cgccaggcgg cgggcgagat
     6061 cctcgaggcg ctcctcctaa gctgcacccg atgtcccggc cccggcaggg cccttgtcgt
     6121 cgatatttaa actctatata gtttataggc taaatcggca tgatggggcg ctcggtcgct
     6181 ccgtcgcgca agaggagccc gaatgtcacc caggtctctg ccggccgtcg tcgtcctgtt
     6241 cctcgccctg ctgccggccg cggtgtccgg ccaggcaggc actggcacca tcgccggcat
     6301 cgtcaaggat tccaccaacg ccccgcttcc cggcgtgacc gtgaccgtcg tcggcgacgc
     6361 gttaggtgcc acgttggagg ccgtcagcga cgagcgaggg gcgtatcgcg ccgacgcgct
     6421 cgctccgggg gcctaccgcg tccagacgac gctcgacggg ttcgagccgt ccgtcattcg
     6481 cgtcgtgctc gaggcggggc agacggcggc ggttgacgtg acgctcacgc ctgcccgcct
     6541 gaccgagaac gtggtcgtga ccgcccgccg cgtcgaagag gccattcagg aagtgccgat
     6601 tcctgtatcg gtgttgagcg gcgagctcgt ggagagcgcc ggcgcgttca acgtgaaccg
     6661 cgtgaaagag ctgattccca cggtccagtt ctactcgtcg aatccccgca actccgccgt
     6721 cacgattcgc ggcatcgggt caccgttcgg tctcacgaac gacggcatcg agcccggcgt
     6781 cggcctgtac atcgacggcg tcttctacgc tcgcccggcg gcggcgacac tcgacttcct
     6841 cgacgtcgcg caggtcgagg tgctgcgggg gccccagggg acgctcttcg ggaagaacac
     6901 gaccgcggga gccatcaccg tcaccagcag acggccgacc ttcacgcccg acgcgaacgt
     6961 ggaagtggcg tacggcagct acgacttcat tcagacgaag gcgtcggtct cgggtccgtt
     7021 cggcaacaag gtggctgcac gggcgtcctt ctcaggcacg gggcgagagg gattcgtcca
     7081 caacacggcg acacgaaccg acaccaacac gctgaacaat ctcgggtttc gcggtcaggc
     7141 gctgatcgcc ccgtcgagga gcgtcgccat caacgtgtcg gtcgatcaca cgcggcagcg
     7201 gccggagggc tatgcacagg tggtcgcagg cgtcgcgccg actctgcgcc cggcgaatcg
     7261 ccagtacgcg cagatcgcgg cagatctcgg atacgcgcca ccgagtttca acgcgttcga
     7321 tcggctcacg gataccgaca cgagctggca gtcggatcag gatcttggcg gtgtatccgg
     7381 gacggtggac tggagcgccg gccgtgggcg ggtgacctcg gtgacggcct accgctactg
     7441 gcactgggac ccgtccaacg atcgtgactt catcggcctg cccgtcacgt cggtctcggc
     7501 ggcgccgtcg aaccagagtc agtggacgca ggaggttcgc tacgctggag ccctgtcgcc
     7561 cagcctgaac ctcgtcacgg gcgtcttcgc gttcaaacag gccctcgact cgaatccctc
     7621 cttcaggcag gagcagggct cggcggcggc acgcttcctc ctggcgccga ctcctgccgc
     7681 cgcgacgccg ggactgctcg acgggtacgg gttcgatcag gacttgaggt accgaaacct
     7741 cagcgcggcg gccttcggcc aactcgagtg gctcgtcacc gatcgcctgc gcgtgctgcc
     7801 tggtctccgc ttcaactacg accagaaaga cgtggacttc aaccagaccg tgtacggcgg
     7861 cttgcagacg accgaccctg tgctcgtggc gctgaagttg tcggtgctgg cgcctcaggc
     7921 ctataccgcc gatgtcgatg acacgaacac ctccgggcag ctgacggtgg cgtacaaggt
     7981 ctctccctcc gtcaacgcgt acggcacgta ctcgaccggc ttcaaatccg tcggtctcaa
     8041 cttgaacggc gtgcccaccg acgcgctcgg caacccgatc ctctcggcgg caaccgtcaa
     8101 gcccgaagac gcacggcacg tcgaattcgg tgtgaagacg gagttcctcc ccggtgtgac
     8161 cgccaacttc accgtgttca acaccgcgat caaggactat cagacgcagg tcgtcaactc
     8221 gcaggtcggc gtgctgcgcg gctacctcgc caacgccgaa aaagtgcgcg ttcgcggcgc
     8281 cgagttcgac ggcagcgctc gaatcaaccc acggctgtcg ttctacggcg ccgttgcgta
     8341 taccgacggc atctacgtct ccttcccgga tgcgccgccg ccgctcgagg acaccggcgg
     8401 actgccggtc aaggacatct cggggtccgt tctgccgggc atctccaagt gggcgttgac
     8461 cttcggcggg gaatacaaca agccggccac ggtgttcaga caggccggcg agttctttgg
     8521 agccgtggac acgagctatc gatcggagtt ctcgtcgagc ccgtcggcgt cgcgctacct
     8581 ggtcgtcgac ggctacggcc tggtcaacct gcgggcaggc tttcgttgga gcgacggctg
     8641 gtcgttgttc ctgtggtccc ggaacctgct cgacgagcag tatttcgagt tcctcacggc
     8701 gcagcctggc aactcgggtc tgtatgtcgg actgccgggg gatccacgca cgatcgggct
     8761 cacgttgcga atgaagctcg ggaacggcca gtgagcccgg cgcagggaga gaccactcgg
     8821 taacaaacct gggacaaatc tccggtagga tgtgcaggcc gaccgacgat ggatccgctg
     8881 tttgcctgga tcgagacgac cgcgctcagc acgtttctcc gagagtcacc ctccctgtgg
     8941 gtcttcccgt tcgtactgat tctgcacacc gtcggtctcg cctttttcgt gggggcgaac
     9001 gtagcatggg acgtccgcct gctcgggttc agcaccggca ttcccgtcga gacgctgcgc
     9061 cgctactttc tcgtgatgtg gatcggcctc gtgatcaacg gggtatcggg cgttcttctt
     9121 ctgattgcct acccgaccaa agcgctcacc aaccctcttt tctacgtgaa gctcgtgctg
     9181 atcggcgcgg gcatctgggc ggcgctgcgg ctcaggcatg acgtccaggg ccgggggagc
     9241 ggcggcgacg cgctcccaac gcgcatccgc gcgctcgcgg gcgcgtcgct ggcctgctgg
     9301 gtggcggtga tcttcgccgg ccgtttcctc gcgtacacct gcacgcgact cacggtggac
     9361 accatatgct agacgagact ctgcggtcga ttcagatgtt cctgtacccg atgccgcagg
     9421 gtgagttgct gcgcgaaact cgctgggcct ggcccatctt cgagtcgctg catttcctcg
     9481 gcatgtcgct gctgctcggt accgtcggca tcttcgatct gcgcctgctc ggcttcgcac
     9541 ggcggatccc tgtcgccgcg cttcacaagc tcatcccaat cggcatcgcc gggttcgcga
     9601 tgaacgccct caccgggatc tccttcctgt cggccacccc ggatcagtac ctcttcaatc
     9661 gtgccttcta ctggaaggtg gcgttcttcg tcgtggccgg cgtgaacgtg atggtgttct
     9721 acgcccgcac gttcaggcgc ctgctcgaca ccgagcccgg cgctccgccg ccgctcgggg
     9781 cgcgcatcgc cggaggcgtc tcgttgatcg cctggattgg cgtcatgtct gcggggcgcc
     9841 tgctgacgtt cttccggccc tgaggggctg accctttacc agcggaaagc cgtccgggag
     9901 atctgttccg ccagccgcag gtgaaggacg tcggccgtgt cccggccgag atagggaatg
     9961 tccacgcggt gggaacgctc gtttcctccc gccgtgtcta cgcggacgct tctcattgcc
    10021 gctcggcggt cgaacggtga ctcgctgcac gcgaccgctt gtatcttcgc gatgggcgcc
    10081 acggtcacgt gtcgccacag ccacccgctg cgaaagacga cggcagtgtc ggtgatggcc
    10141 cacgccatgt gctccacatg tttccgggac gcgacgacgg cccacgcgat ggtgattggc
    10201 gtcgcggcca gccaccagac agacaggaag aagaccaaag gcacgaccgg ggcaagcgcc
    10261 acgaacaggt atggcttgag ggcccgccga aatgccctgg ggtggatggg ctgccacgcg
    10321 agtgcgccga tgtcgaatcc cgggagcacc tgccggacca gcgccggcac acggtccgtc
    10381 tcgacgatcg gggccagccg ctcgcgttcg aagggacgcg cgccgccttc agcgggtccg
    10441 tggccgccgg ctgtttcggc ccggaccgac atgcggccca ccaggcgctg cagcggcgtt
    10501 tcccggatcg tcagcgtctg gactcgcggc agcgggattc tcgtgctcac gcgcgtcagc
    10561 aaaccatact cggcgcgaag gtcctgtccc gcgagcgaga gccggaaatc gtagagccgc
    10621 gtgacggccc acgccatcga gaccaggcgg acgagcacga ggatgccgac gagacccgcg
    10681 atcaggatcg cgacctggac gatcgtcacg gcgtgccccg attccacctc ctgcagcgcc
    10741 tcgttgacca ggcgcggtgc gtcccagccc ccggtcagcc gttgccacag cgctgtgggc
    10801 agtccgaact gccacagcgc tccgtatgcg gctccgatga ggatgaggcc gcggttttcg
    10861 aggaagccgt ggagcagcag ctcgcgcagg ggcaggtgca gcagcgtgag cgcttcgtcg
    10921 ggcgccgcga ccggcgcctc atccgccgtg gcctcgcggg gcccgcctgc ccgcgcgcgc
    10981 ccctcgaaca cgcggcgccg catctcgtcg aacaccctgt cgtcgagcac gctgatggtg
    11041 gcttccggct cgttgccccc gcctgtctcc acgcggacgt tggtcacgcc caggagccgg
    11101 tgcacgacgt tgcgggaggt atcgaggttc tgaatgcgca ggtacggcac gtgccgctcg
    11161 tttcgaaaga ggatgcccga gcggatgacc agctccgtcc cctcgtagcg cacgcgaaac
    11221 gacaggtagc gcgcaaccgc ggcgatggcg cccggcacga gaagcaggag catccagaac
    11281 tcgccattcg agatgtcggc ccacggcatc catcgcccga cgaagggggc cggctcctgc
    11341 gatcgtccgg cggtgaagta gaccaccagc gcgggaagcg cgaacgtctt caaggttccc
    11401 gcgaacgaaa agagcatcga cgaggggtgg agacggtgct cagacggcat cgccttcgtc
    11461 tttcggcagc aggtgggcgc ggatgcgccg cgccacctcg tgatcgaggc cttccaggtt
    11521 gacgcgtgag tgagccatgc cggcggtgta gatgacgagc gtgccaagtc cgtagcgacg
    11581 ctccagcggc ccctgcgaca cgtcggtgtg ctggacgcgg gagcgaggca cgttggtgat
    11641 cgtccgccag tacacgccgc ggcggatttc tatgccgagc tcgtcgatgc ggtacgacgc
    11701 gaagcgatag gcgatcgcag gccagcgctg gaactgccag gcgagcgcga cgacgccagc
    11761 cagccacagc ggcatgagca gcaggccgag cgccagtatc ccgctgtcgg cccagacgaa
    11821 ggtgacgaag accagcgaga tcgcggacag gatggccgtc gggatccagc cgagcgtgcg
    11881 ttgcagcggg atcacgcgtg gatcgagctt gcgatccacg ccgtcggcaa tcgattcgac
    11941 cgcgtcggaa tccgaatcca tccctgaaga gacacagtaa cagacgagga gccggcccgt
    12001 ctggttccta ccggaagtgc ttcgtggact gctcgttctc gaggcagacg aactcgatca
    12061 gctccgtgtc gagcatcacc tcgttccgta tggtcaccgt ccacggcttc gtgtacgcct
    12121 tcggatcgtc cacggtgact tcgatgtcga gcgcgccgta gttcacacgc gtgaagcgct
    12181 ccgtcatcgt cgcggcatcg gtcagcggac tgccgttgac gtccagccac ccgccgtcgc
    12241 ggaacccgct cgtctggacg accagcgtgt cgcccatcca tcgtcccgtc gagtagccga
    12301 accaccaggg ctgcggatcg ttgcgcggga gggggcgtcc gtccgtgaag atctgccgga
    12361 tcccggcgtt gccttcgtag aggatcacga tgagagccgg ggtctggatg atcttcctcg
    12421 gctgaggatg ggtgtacagc tgcatgttgc ccatcggcag gcagtgcgcg tctgggttgt
    12481 ccttgctgtt gccggccatc cgctgcttga ggagctccgc tgcccagggc tgcagcggca
    12541 ggccgccggc aacgccggaa gcgatgttga agaagagccc gggacctcgc ggaagcacgc
    12601 tgggcgtgcc gggcgcgccg ccagtcccgc tgacgcccgt tcccgcgccg atctcccgcc
    12661 agccggggtt cgaccagatg ccggagagat ccggtttgcc atcagaggct cggggcgcgg
    12721 gcgcaagcag gttcggcctg ccgtcggcgg tgcgcggcac gttcggtgtg gggtacgcgg
    12781 gccactgagc cgagggggat gccgtggtcg cggcggcgaa cacacacgcg gccgcgacac
    12841 gactggcgat cgcgatcgac atcagtcccg gattatagcg tcgccccgag gcccgagccc
    12901 tcgccctgag gcctgagccc tgggccctga tcctagttac ctgtgtactg gatgacgaac
    12961 aagccgccct gcaggtcgct cgccacgatc aggtcgctcg acggaaagat gtaggcgccc
    13021 cagttcccag cgaattgact cgagctcccc tcataggtgt cgaagaagcc cagcacgcgc
    13081 agttgtgccg ggttgctgac gtcgagcagg tgcacaccct ccgtgtagtg cgacacgacc
    13141 atgcgactgc cgtcgatcat gacgttgtga ggaatcgttc ccggctgagc gatgtactcc
    13201 gacacttttc tcgggttccg catgtctgcg agatcccacc cttcgagcgg cctccccgcg
    13261 cgctcgtcgg tcgtgaagag gtaccggccg tcggccgtca gccacgagtt gtgggtgaag
    13321 cggccccccg tgaagaaccg cgtgagctcg gtgatccgcg ccgggttcga gatgtcgagc
    13381 acggccacga atccgtcgta gatcgccgac gcgaacaggt agttgccccg gacgtagcag
    13441 tcgtggatgt agaagtcggt aaagctgccg gcctcgcgag ggtcggccgg gtcgcgctgc
    13501 acgtcgagca cccgcatgcc aatccgctcg ccgttgtgat cggcggtgcc gttgacgaac
    13561 agcaatcccc gggcatcgtc cacgcacagc gtgtgagcgg agaagaacgt gtcgctccac
    13621 gtgcgcagct tctcgggatg gtccgggttg cgaaggtcca cgatgtccag gccgtggggc
    13681 gcctctgtcg tgacgtaggc atagggtccc cacgttttca cctcgcgcca ctggctggcc
    13741 gggccggcga tcagcgtgat gttgcgcggc tggtcgggac gcgtcacgtc cacgatcgac
    13801 agtccagccg aagtgccggt gagggcgaac cggcgtccgt tgggggctgt gtagccccag
    13861 ttgccagagc cggagagcgc ccccagacct tcaatcggct cgtcgtgatg gagggcgtgc
    13921 agcggcggcg ccgacatcag cgcgttgagg tccacgcgag ccatcaagcg catgttgtac
    13981 gtctgcgtcg ttgccgtggt cggcggtgtc gatgaattcg actgcagccc cggttcggaa
    14041 ccgtcgcacg ccgccaggct cgcgatcagc atgacgccga ctgcggcccc ggcgcggccc
    14101 gtctcgatcg ccatacgcag gtggattata gcgacgccag gaatccagaa tcccgagccg
    14161 gtatactaac cgtcggtcgc tcgcatcgca gcgggagtaa gggagggagt gccggttggc
    14221 ctatatcatt tgcgagccgt gcgtcggcac gaaggactcg gcgtgcgtgg atgtctgtcc
    14281 cgtcgactgt atccacccca ggaaggatga gcccgggttc gagtcggcgg agatgctgta
    14341 tattcacccc gacgagtgca tcgactgcgg cgcctgcgtg ccggcgtgcc cggtcgaggc
    14401 gattttcgcg ctcgacgaaa cgccgcagaa gtgggcgaac tacattccca tgaacgccgc
    14461 cttctatcag aagtcgtagc cggatacggc aatcgggtgg gtcgagtcca cattcacggt
    14521 taggggtttg ggacaatgcg gatgtcgagt tcgctgtcac gtcccgcgca ggccctgacc
    14581 tgcgcgttga gcctcctcgt cggatcctcc ctgtcgcttc gcgccgggca ggacgccgat
    14641 gccagcctcc agcttcacct cgccaccctg ctctacgacg agacacggta cctggaagct
    14701 ctcgatgcat tcaccgacgc aaccaagacg acggatccgc ggctcggtgt cgcggcccga
    14761 aagggcaccg tccggacgtc cctcaaagtc gccgagttca agcgcgcgcg cgaggaagcc
    14821 gagacgcttc gcgggctcgc tcccgacgac cccgaggcgc tgacgctcca tgccgacgcg
    14881 ctctggtcga tgggcttgtt cgacgaggcc gagcgggaat accgggacgc cctggcccgc
    14941 acgccgggat cggctcgcgc ccggtacggc ctggcacggt cgcttgctgc cagcagccgg
    15001 ctgagcgacg cgctcgactc ggcgcttgcg gcgtcggcag ccgctccacg cgacggcgag
    15061 attcacgcac tcatcggcga catctacgag cggctgcatc agttcgatca ggcggccaac
    15121 gcgtaccgca actacatcaa cctgctgccc aacaaggatc gcagcgagaa ggccgcgtgg
    15181 gcaaaggcgc aggtgagctt cctcgattcg ttcagcggcg tggtgcccgt cgagatcgac
    15241 cggctggacc tcgagacgct ccacacgctg ccgttcaggc tcgtggacga caagatcatc
    15301 gtgcaggcca gggtcaacgg cggccagtcc cgcgaattca ttctcgatac cggctccgag
    15361 gagaccacgc tctcgcgcga aacggcgcag cgcgaggggg tccggggggt gaccttcacg
    15421 ctcagcgcag gtgtcgggga aatcgggctt cgcgggctgc agctcgccca ggcggagtcg
    15481 ctcgacctgg gcactcttca gatccggaat cttccggtgc tgatcaagaa ccctgcgctt
    15541 cggggcatcc ccaagcggga gggcaattcg ttctcaccgc tctcgatggg gatgtcgctg
    15601 cggatcgact acgagcgacg gctgctcacc ataggcaagc ggctcccccc ggtcgaggcc
    15661 gacatccgcc ttccgatgcg gatcaatcgc ctggccatgg tgcggggtct cgtcaactcg
    15721 acccaccccg cctacttcgt cgtcgacacg ggcggccagg tgatctcgat cagcgcagaa
    15781 accgcgagcg cgctcgctcc gtcccagttc cggcgtattc cgttgaaggt gtgggggaca
    15841 tccggctggg accgcgatgc gttcctcctg ccgggcgtgg acctggactt cgacgagatc
    15901 gaataccgca atttctcgct cgtcgtgctg aacctgcgcg cgcccagcat cctgctcgga
    15961 tttcagctgg gcggcatcgt ggggcacaac ttcctggcgc cgttccgcgt cacgatggac
    16021 ctcgaaaaca gcgagctgct gctcgagcga ctgtgagacg ttcactcttc ttgacatcgt
    16081 aggggccggt ctttagaccg gcccaggcgg gtctgaagac ccgcctctac gtcaacggaa
    16141 gtgaactccg gtggttagaa gtcgtacccg atccagatct tgaactgcac tcggcggaac
    16201 tcctcgctcc cgccgtacgg cctgaagagc gcgtcctccc attcccggtt gaacagcgtc
    16261 ttccacgacc aatcgaggtg caccgggaag cccaaggcca ttgtctggat gcccatgccg
    16321 tacgaggccc gtgagtccac gagccggagc cccgaaatca cctgcgggac gccgtagacc
    16381 ggcgtttgct gaccgaggcc ggggcccgga acgaagccga cgacgggcgc gtacagctgc
    16441 gagtcgttct ttgcccaggt gaacggctgg ccgttgaaat agccgccgcc gaagttcgca
    16501 tagaagacgc cgcgaacgcc gccgaggacg ccgatcggcg tgagcatggc gttgatgagc
    16561 gggaagcgaa gctccgcgtt gaagaacgcc gccgtgtcgc cggcgaactg gagatactcg
    16621 taaccgcgca tctcggagtt gccgccgaag tacatgaagt caggcgagtc gccccggctc
    16681 ctgaacgcgc gggcgcgaag cgcgagcacc cccgtcgagg ccaggcgctg gtagtaccgg
    16741 gcatcgacgt cgaaggtctg ccgcgtcagc gtgttgccga tcgggggcgc caattcgtac
    16801 gacagccgca tcgtgctgcc ggacacgggc ccgaactccc ggaacacggt ggtctcctgg
    16861 atgaaattga cgcccatcgg cacgaaggtc cccgaacgaa acagctgacg cccgtagttg
    16921 tcctgctggt actgctcgga gtattcgtcg aggaccggat cgttgaagct ctcgttgtaa
    16981 ttggccaccc cggccgtcac ctccagccgc cggtagcggc tgagcggcca gatcccgtag
    17041 accgatccgc cgcggaccga ccgcgtcgcg atcgccaggt cgcggtccac caggcccgag
    17101 tacacggggt cgtagaacac gcccgccatg ttcccgtaga agaactccgt ctgcgagtac
    17161 gcctgcagca tgtagttgaa gcggctcgcc aggttcgtgt acgcgaacga catcgttcgg
    17221 tactgtgaaa tcgatgcgac gtagacgctg aactgctggt cgccgagcac gtcgctgaag
    17281 gtcaccgccg tgccgccgaa cacatccccg ctgctcgtca cgcccacgct cacgggggga
    17341 cggccgtcga ggaacatgtt ctcgaacttc cccttcttct tcttgttcga ggcgacgatc
    17401 gtgtgcgaga gcggggcctg gaagtcgatg atcggccccg gggccccgaa atcggaggag
    17461 gccgccgtga cgatcggatc gcgcctgtcg atgaggtgca gctcgtactc gcccttgtga
    17521 tacgtcacga cgatcaaccg gggcgtcgcg gtgtcgcctt tcagcacggc cgtatagaga
    17581 ttcccgccca cggcatccgt gtactgccgc agctcgcccg tcttcagatc gagcatccag
    17641 acgttgtaga tctgcccgtt cctcgccacg tcggggtcga tcggctgcgc cgggtcggtg
    17701 gccgtggacg ggaagacgag cgtgttgtcg tcgaggaagt gcgcagtcgc gtcgtcgtgc
    17761 gttccgaacg tgagctgtgt cttctcgccg gttgcgaggt ccacccggaa cagcttctcg
    17821 ttgccgctga tgcgggccag gtacacgatc gagcggccgt ccggcgccca cgtcggcccc
    17881 gaatcggcaa aaccgtcctt cgtgatgttg gtgacctcct cggtttggat gtccaccacg
    17941 aagatgtctc ctgttccgcc ctgcagggcc gcgaacgcca cgcgccgtcc atcaggagaa
    18001 aaatcgggcg actcggggtc gtccaccgtc ttcatgtcga tgcgcttcac gatttccctg
    18061 gtgatcacgt tctggaggat caggctgcga gacttttcgg accgcgcgaa atagcccagg
    18121 gtgtcgtccg acgaccagga cagctgcgga accgtcaccc agcggttgcc cgacgtcgcg
    18181 atgtattcga acccgagctt gtagtcgaac ccctgggtca ggttccggaa ctcgctgccg
    18241 tcccgcgcgg agatgatgac gatgtctcct tcctggtcgc gggcattgcc cgtgaagatt
    18301 gccagcaggt cacccgaggg ggacggttcg accgtgtagg cgccccggta aggcgacctc
    18361 tccgggttgg gtacgagatt ccggccgtag tcggccggac gctccttgtc cctgaacggc
    18421 ttgaactttt ccttcaggta cttgtcgaac tccgaatcga actcgtccgc cgtgatcttg
    18481 aaggtctctt cgtaggcgtc ttcgcctccg ccaatcaccg acttgcgcag cgagaagagg
    18541 tactggcgga tgccttcctt accccaccgc gcctcgatga actcgaagat ggcatgaccc
    18601 aggttgtaga tcatgcgcgg cccgccgacg tcgctgtatc cctcgagcct cgtcatcttc
    18661 gggatgatgt cggcgacggc cgcgtcgcga accgacatca ggtcgagcgg gcgccagaac
    18721 cccgtcatgt aatccgcgag tccctcgtgg agccacaggg gcgtcgtcct gcgcaccagc
    18781 gacgccggaa tgatgtcgta atcgaactga tgggtgagct cgtggacgat cagccggtag
    18841 agcaggtcgg gcggctcgtc catcggcatc acaatgcgct gacgggtcgg ctccgcaaac
    18901 gcgcccacgc cttcctgggc cgcgcccggg atcacgttct gttgctggaa ttcgctgctc
    18961 gtctgaaaca ggatgagcgg gaccttgtgc gcgaggtcgt gcttgagctc cgagctgacg
    19021 tgctgatacg cgctctccgc gtaggacgcc atgcgctcga gatgcggctc gatctcgggg
    19081 tagtagtaca tgatgaagtg gtccgtctcg tagtggtacc actcgaaatt gtcgtaacgg
    19141 atctggtttt tcccgtagta ggggacgtac ggagtctggg ccgtggcggg gatcgcatac
    19201 gccagggccg cggctgccga cacgacccgg agcagggt
//
LOCUS       DBNGSH010000442         6727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4116186, whole genome shotgun sequence.
ACCESSION   DBNGSH010000442 DBNGSH010000000
VERSION     DBNGSH010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4116186"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..484)
                     /locus_tag="AC16CY_10165"
     CDS             complement(119..484)
                     /locus_tag="AC16CY_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3354196.1"
                     /translation="MPVRLAAADPQRQGRRGVTRGGHACAGSLFRWRPAPGVRPAVGR
                     HNISNIVIKVDGNKATGRAYWFHYSNDNPKRSGVFDGFGHYEDEMVKVKGQWYFTKRR
                     IYNENRDEWAYKAGKNPAW"
     gene            complement(430..1542)
                     /locus_tag="AC16CY_10170"
     CDS             complement(430..1542)
                     /locus_tag="AC16CY_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HIR3354197.1"
                     /translation="MSDRVVLTIGTKKGVFVAESKKPRRSFSLRGPFGMGIPVYATFI
                     DTRGTPRIFAGSCNPFFGMKVLRSTDMGKSFKETKSAPAFSKEDGRALANIWSIEAGA
                     GKKELLSGVEPASLFRSPDGGDTWEIVPGISDHEHARKWSPGNGGLCMHTIIRDGNRV
                     HLGISTGGHYLSEDGGQTFQASNQGVGAGFAPDPYPEFGQCVHKIVRHDDAPGRMYMQ
                     NHGGWAKWTGPGGPRPDIGVLRSDDYGHSWRSIAKGLPSDFGFPIVVHPHDADTVYVM
                     PLEPETRTCPGGAPAVWRSENGGDSWGRLSKGLPKKQTYYTVLRDAMSIDDLKSPALY
                     FGTTTGQLWIGRDGGEKFECLFDSLPPIHNVKVAVV"
     gene            complement(1545..1832)
                     /locus_tag="AC16CY_10175"
     CDS             complement(1545..1832)
                     /locus_tag="AC16CY_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3354198.1"
                     /translation="MGSPPTVTIYIVGPLRPYCAGRSQLSIEAQTVRDALADLERKEP
                     TLYSNLCDETGAVRRHLNVFVNSDNVRDRNGIDTTLTPGDVLTFLPAVSGG"
     gene            2027..2968
                     /locus_tag="AC16CY_10180"
     CDS             2027..2968
                     /locus_tag="AC16CY_10180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3354199.1"
                     /translation="MKSSALRQPPDDDLLDAYSSAVVSAVETVGPSVVRVERKRSGGS
                     GIIFTPDGFVLTNSHVVDGAATVSVTLADGRTVRGDVAGDDPSTDLAVLRIEATSLPW
                     AGLGDSRGLRVGQIAIAIGSPFGFHHSATAGIISGVGRSLRARTGRLMDDIIQTDAAL
                     NPGNSGGPLVTSRAEVIGVNTATILPAQGLCFAVASNTARLVAAHLISDGRIRRSYIG
                     VAGQKVPVPRLPGVQALTAVSSGVMVLSVEKGSPARKAGIVSGDVIIAFGDRPVAGID
                     DLQRALTSERIGAPGVLTILRNGVRQELPIVPAEAPG"
     gene            3038..3271
                     /locus_tag="AC16CY_10185"
     CDS             3038..3271
                     /locus_tag="AC16CY_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3354200.1"
                     /translation="MAKPHSIHAAKTQLSRLIERAERGEEVVIARGSTPVARLTRLRA
                     ERPARRFGAMRGRARVTRAFFEPLPESELKGWK"
     gene            3275..3661
                     /locus_tag="AC16CY_10190"
     CDS             3275..3661
                     /locus_tag="AC16CY_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3354201.1"
                     /translation="MRLLLDTHALLWWLDGDRRLSLKARRAIANQSNTILVSAASAWE
                     IATKARLGKLPGATDVAADVAACVAGQGFLPLDITMLHAQKAGRLPGHHRDPFDRMLI
                     AQSQMEDIALLSDDEAFDNFDVRRFW"
     gene            complement(3686..4174)
                     /locus_tag="AC16CY_10195"
     CDS             complement(3686..4174)
                     /locus_tag="AC16CY_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR3354202.1"
                     /translation="MAQPGVVVAFSSRCGSTETIAHAAAVGAVNARALPRLRRLADDG
                     PVAAECAETLRRMKKEYVPPTEADIAGASALMIVPTAGMTPASGVWQPLIALLDKLSS
                     AGQLVGKVGAVIDTGDSETVAGFSSALAARGFAVIAATGDARAHGRAVGAAIVAQSRP
                     KQ"
     gene            complement(4182..4880)
                     /locus_tag="AC16CY_10200"
     CDS             complement(4182..4880)
                     /locus_tag="AC16CY_10200"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008127 - quercetin 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3354203.1"
                     /translation="MITIRRSADRGKADLGWLNSHATFSFGSYYDPNFMGFRALRVLN
                     EDRIAPGKGFGPHSHRDMEILTYMVDGRLAHRDSLTGAHILGRNEVQVMAAGDGIVHS
                     EFNASDTESSHSLQIWIEARAEDLTPSYQQIAFTPEDKRGRLHLLAAPEAAEPARTAV
                     INQDARVYVTEVADNDRVTHELPDGRHAWIQVVRGAIDVNGTPLQQGDGAAISDERAV
                     VVSGRGEALLFDLP"
     gene            complement(4943..>6727)
                     /locus_tag="AC16CY_10205"
     CDS             complement(4943..>6727)
                     /locus_tag="AC16CY_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3354204.1"
                     /translation="SRRAKGVTVVGRLKPGVTVAAAERDLASVTSRLTDLYPDANYAI
                     FGFSLHATGLVSRVSGNLRQPLLFLLAAVGVLMLIACANVSNLLIARASARRKEMSVR
                     AALGAGRLRVIGQLMTESLVIAALSGALGLALAFVLVKLFELYGPAGLVPVAGLGING
                     WVVAFAIGISSVTSVLFGLIPALTTSAGLNDVLKESARGATAGRRRFRESMVAFQVAA
                     SLVLLISAGLLIGSFIRVQQADLGFDSRNVLTFELQLPVSHYGEPERRMFLYESLQSR
                     LRAIPGVVSVGAADRIPFGLQGGSTFRVVGRVVDPGSPQPTVRPARILPGYFESLGVP
                     VRKGRDFTAADTRDAPRVAIIDEATARRFFPNGEDPIGQQVNDVEPGLTATIVGVVGS
                     VKRRDPATPPEMSVYHAATQTAGAAMTFTVKTATDPLAVIPAIRQQLSELDPLLPLIR
                     TLTMEQRVSDSMARRRLSMQLMSFFGLAALLLAATGLYGVLSYVVNQRRREVVIRVAL
                     GARPRQVIELVARQGMLSVAIGLSVGLASAVAVARLLRTGLYEMSPTDPVVYMSVTGL
                     LVLIAIAAIAVPARRAARVNPVVALREE"
BASE COUNT         1149 a         2264 c         2172 g         1142 t
ORIGIN      
        1 accgagcggt acctgctcca gagcggcgag gagaacaccg cccgcgccga gtacgcgaac
       61 tatcgccgct tccgcgtcga gaccagcgac gacgtgagcc cgaagtaggc cgcgccgcct
      121 accacgcggg gttcttgccc gccttgtacg cccactcgtc gcggttttcg ttgtagatcc
      181 gccgcttggt gaagtaccac tggcccttca ccttcaccat ctcgtcttcg taatggccga
      241 acccgtcgaa gacgcccgag cgcttcgggt tgtcattcga gtaatggaac cagtaggcgc
      301 gcccggtggc cttgttgccg tccactttga tcacgatgtt cgaaatgttg tgtcggccca
      361 cggctggacg caccccgggg gcgggccgcc agcggaacag agagcctgcg catgcgtgtc
      421 ctcccctcgt cacaccacgg cgaccttgac gttgtggatc ggcggcagcg agtcgaacag
      481 gcattcgaac ttctcgccgc cgtcacgacc gatccagagc tgaccggtcg tcgtcccgaa
      541 gtagagcgca ggcgacttga gatcgtcgat cgacatcgcg tcgcgcagca cggtgtagta
      601 ggtctgcttc ttcggcagcc ccttcgagag ccgtccccac gaatcgccgc cgttctcact
      661 gcgccacacc gcgggcgcgc caccgggaca cgtgcgcgtt tccggctcga gcggcatcac
      721 gtacacggtg tcggcgtcgt gcgggtggac cacgatcgga aacccgaagt cggacggcag
      781 gcccttggcg atcgatcgcc acgagtggcc gtagtcgtcg ctgcgcagca cgccgatgtc
      841 cgggcgtggc ccgccgggcc cggtccactt cgcccatccg ccgtgattct gcatgtacat
      901 ccggccgggt gcgtcgtcgt gcctgacgat cttgtgtacg cactgcccga actcggggta
      961 gggatcgggc gcgaagccgg cgccgacgcc ctggttcgac gcctggaacg tctggccgcc
     1021 gtcctcgctc aggtagtgcc cgcccgtcga gatgccgaga tgcacgcgat tgccgtcgcg
     1081 aatgatcgtg tgcatgcaga ggccgccgtt gccgggggac cacttccgcg cgtgctcgtg
     1141 gtcgctgatg ccgggcacga tctcccacgt atcgccgcca tcggggctcc tgaacagcga
     1201 tgccggctcc acgccggaca gcagctcctt cttgccggcg cccgcctcga tggaccagat
     1261 gttggccagg gcccggccgt cctctttgga gaatgccggg gccgatttcg tttccttgaa
     1321 gctcttgccc atgtcggtcg agcgcaggac cttcatgccg aagaacggat tgcagctccc
     1381 ggcgaaaatg cgcggcgtgc cccgcgtgtc gatgaaggtc gcatacaccg ggatgcccat
     1441 cccgaacggc ccgcgcagcg agaagctgcg ccgcggcttc ttggactcgg cgacgaacac
     1501 acccttcttg gtaccgatgg tgagaacaac tcgatccgac atcttcagcc tcctgagacc
     1561 gccggcagga aggtgaggac gtctcccggc gtcaaggttg tgtcgatccc gttccgatcg
     1621 cgcacgttgt ctgagttcac gaacacattc aggtgccggc gaacggcgcc tgtttcgtcg
     1681 cagaggttgc tgtacagggt gggctctttt cgctccaggt ccgcgagcgc gtcccgcacc
     1741 gtctgtgcct cgatggagag ctgggaccgg ccggcacaat acgggcgcaa tgggcccacg
     1801 atgtaaatcg tgacggtcgg cggcgaaccc atggcgaaaa tatcgagctt accaccaccc
     1861 gttgtcagcc gtgttgcttg tacgtatgac gccgacgagc tttgcctcat caactctcat
     1921 ctgccccctg ccccctgccc cctgccccct gcttttgggt gatgcgcggg ccctcgccac
     1981 ccgcgtacga ttcatgtgtc tcggtccctc gataacggca ggccatatga agtcctctgc
     2041 tcttcgccaa ccgccggacg acgacctgct cgacgcctat tcgagcgcgg tggtctcggc
     2101 cgtggaaacc gttggtccat ccgtcgtgcg cgtggaacga aagaggagcg gagggtccgg
     2161 catcatcttc acgcccgacg gcttcgtgct gacgaacagc cacgtcgtgg acggcgcagc
     2221 cacggtcagc gtcacgcttg ccgacggccg gaccgtacga ggcgatgtcg ccggcgacga
     2281 tccgtccacc gatcttgcgg tgctgcggat tgaggccacg tcgcttccgt gggccggtct
     2341 cggcgattcc cgcggtctcc gcgtgggaca gattgcgatc gcgatcggaa gccccttcgg
     2401 cttccatcac tcggccaccg ccggcataat cagcggcgtc gggcgctcgt tgagggccag
     2461 gaccgggcgc ctgatggacg acatcatcca gaccgacgcg gcgctcaacc cgggaaactc
     2521 cggcggaccg ctggtgacgt cacgggccga ggtcatcggc gtgaatacgg cgacgattct
     2581 gccggcgcag ggtctctgct tcgcggtggc cagcaacacg gcgcgtctgg tggcggcgca
     2641 tctcatcagt gacggccgca tccgccgcag ctacatcggc gtggcggggc aaaaggtccc
     2701 cgtgccgcgg cttcccggcg tccaggcgct gacggccgtg tcgtcgggcg tcatggtgct
     2761 gtcggtcgag aaaggcagcc cggcgcgcaa ggcgggcatc gtgtccggcg acgtcatcat
     2821 cgcgttcggc gatcgtcccg tggcgggaat cgacgatctt cagcgcgcgc tgacgagcga
     2881 acggatcggt gcgccgggtg tgctgacgat cctgcgcaac ggcgttcgcc aggagctgcc
     2941 gatcgttccg gcggaagcgc ctgggtagtg tccggcttcc ctacgccagg cgctcggctt
     3001 gcttagctaa gctgacctag ctaagatcgt agggaccgtg gcgaagcccc attccattca
     3061 tgccgccaaa acccagctct cgcgcctcat cgaacgcgcg gaacgcggag aagaagtcgt
     3121 catcgcgcgc ggatcgacac ccgtcgcgcg tttgacccgc ctgcgggcag aacggcccgc
     3181 ccgccggttc ggcgccatgc gcggccgcgc ccgcgtcacg cgggccttct tcgagccgct
     3241 tcccgaatcc gagctcaagg gttggaagta gcgggtgcgc ctgctgctgg atactcatgc
     3301 actgctctgg tggctggatg gcgaccgccg gttgtcgctg aaggcacgcc gtgcgatagc
     3361 caatcagtcg aacaccatcc tggtcagcgc cgcctccgcg tgggagattg ccacgaaggc
     3421 gcgcctcggc aagctgcccg gcgcgacgga tgtcgcggcg gacgttgcgg cgtgcgttgc
     3481 tggccaaggc ttcctgccgc ttgatatcac gatgctccac gcacagaaag cgggtcgact
     3541 gcctggccac caccgagacc cgttcgaccg aatgctgatc gcgcagagcc agatggaaga
     3601 catcgcgctt ctcagcgacg acgaggcctt cgacaacttc gacgtccgcc gattctggta
     3661 gcggtaccgg cggggccggg ttcctctact gcttcggcct cgattgagcc acgattgcgg
     3721 cgccgaccgc gcgtccgtgc gcccgggcgt cgccagtggc ggcaatcact gcgaacccgc
     3781 gcgccgcgag cgctgacgag aagcctgcca ccgtttcgct gtcgcccgtg tcgatcacgg
     3841 cgccgacctt tccgaccaat tggcctgcgg acgacagctt gtcgagcagg gcgatcaacg
     3901 gctgccagac accggacgcc ggtgtcattc cagccgtcgg cacgatcatc agggccgacg
     3961 cacccgcgat gtccgcctcg gtgggtggca cgtactcctt cttcatccgg cgcagcgtct
     4021 cagcgcattc cgccgcgacc gggccatcgt cggcgagccg gcgcaggcgt gggagtgcac
     4081 gcgcgttcac ggcgccgacg gcggccgcat gggcgatcgt ttcggtgctg ccacaccgcg
     4141 acgaaaaggc gacaacgaca ccgggttggg ccatgagcgt ctcaggggag atcgaagagc
     4201 agggcctcgc cccgtcccga cacgacgacc gcgcgctcgt cgctgatggc ggcaccgtcg
     4261 ccctgttgca gcggcgtccc gttgacgtcg atggcgcctc gaacgacttg aatccatgcg
     4321 tgccgcccgt ctggcaattc gtgggtcacg cgatcgttgt cggcgacttc ggtcacatac
     4381 acgcgcgcgt cctggttgat caccgccgtc cgcgcgggct ctgcggcctc gggcgccgcc
     4441 agcaggtgca gccgcccacg cttgtcttcc ggggtgaacg cgatctgctg gtacgacggc
     4501 gtgagatcct cggctcgtgc ctcgatccag atctggagcg aatgcgacga ctccgtgtcg
     4561 gaggcgttga actcgctgtg cacgatgcca tcgccggcgg ccatcacctg gacctcgttg
     4621 cgtccgagga tgtgcgcgcc ggtgagactg tcgcggtgcg ccaggcggcc gtcgaccatg
     4681 taggtcagga tctccatgtc gcgatggctg tgcggcccga accccttgcc gggcgcgatg
     4741 cgatcctcgt tgagcacgcg cagggcgcgg aagcccatga agttcggatc gtagtagctg
     4801 ccaaacgaga acgtggcgtg ggaattcagc cacccgaggt cggccttgcc gcggtctgcg
     4861 ctcctgcgaa tcgtaatcat gcggtgatct caatcgtacg gcgagaaaaa agcaagcggg
     4921 gtcggccggg tgattgcgga cctcactctt cccgcagagc aaccaccgga ttgacgcgtg
     4981 ccgcccgacg tgctggaacc gcaatggcgg cgattgcgat gagcaccagc agtccggtga
     5041 ccgacatgta cacaactggg tccgttggac tcatttcata gaggccggtg cgtagcagcc
     5101 gcgcgaccgc gacagccgac gccagtccga cgctgagtcc gatcgccacc gacaacatcc
     5161 cttgcctggc gacgagctcg atcacctgtc tcggccgcgc ccccagcgcg acgcggatca
     5221 ccacctcgcg acggcgctgg ttgacgacgt acgacagcac tccatagagg cccgtcgcgg
     5281 cgagcaggag agcggccagc ccgaagaagg acatcaactg catcgacaac cgtcgtctcg
     5341 ccatggaatc ggagacgcgt tgctccatcg tcagcgtgcg gatcaacggg aggagaggat
     5401 ccagctcgga cagttgttgc cggattgccg gaatgactgc gagcgggtcg gtcgcagtct
     5461 tcacggtaaa cgtcatcgcg gcgcccgccg tctgtgtggc cgcgtgatag acagacatct
     5521 ccgggggagt ggcgggatct cgccgtttca ccgatccgac cacgccaacg atggtggcgg
     5581 tcagaccggg ttcgacgtca ttcacctgct ggccaatcgg gtcctcgccg tttggaaaga
     5641 acctgcgtgc ggtggcttca tcgatgatcg cgacccgggg ggcgtccctg gtgtcagccg
     5701 cggtgaagtc gcggccttta cgtacgggca ctccgagcga ctcgaaatac ccgggtagaa
     5761 ttcgcgcagg acgcaccgtc ggttgaggcg acccggggtc aaccacgcgg ccgacgacgc
     5821 gaaacgtgct tccaccctga agcccgaacg gaattcgatc ggcggccccg acggacacaa
     5881 cgccaggaat ggcccgaagc cgcgactgca acgattcata caggaacatt ctccgctcgg
     5941 gctcaccgta gtgagacacc ggtagttgca gttcgaaggt caacacgttc ctggaatcga
     6001 atcccagatc cgcctgctgc acgcgaatga agctgccgat gaggaggccg gcgctgataa
     6061 gcagaacgag cgaggccgca acctggaacg cgaccatcga ctcgcggaac cggcgacgcc
     6121 cggcggtggc tccacgcgcc gactccttga gcacgtcatt caatcccgca ctcgtggtga
     6181 gcgccgggat caggccaaag agaacgctcg tcaccgacga gatgccgatc gcgaaggcca
     6241 ccacccaccc gttgattcca agccccgcga cggggaccag gccagcgggc ccatagagtt
     6301 cgaagagctt cacgaggacg aacgccaacg caagtccaag agctccagac agtgcggcga
     6361 tcacgaggct ttcggtcatg agctgaccga tcacgcgcag ccggccggcg ccgagagccg
     6421 ctcggacgct catctccttt cttcttgccg aggcccgcgc gatcagcagg ttcgacacgt
     6481 ttgcgcacgc gatcaacatc aacacgccca cggctgccag cagaaacagc aaaggctgac
     6541 gaaggttgcc ggacaccctg gacacaagcc cggtcgcgtg gagggaaaac ccgaagatcg
     6601 cgtagttcgc atctggatag agatcggtca gcctcgacgt cacgctcgcc aaatcccgct
     6661 cggcagctgc caccgtcacg ccaggcttga gccgcccgac cacggtcacg cccttggcgc
     6721 ggcggct
//
LOCUS       DBNGSH010000443         4645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3776302, whole genome shotgun sequence.
ACCESSION   DBNGSH010000443 DBNGSH010000000
VERSION     DBNGSH010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3776302"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="AC16CY_10210"
     CDS             <1..577
                     /locus_tag="AC16CY_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR3354205.1"
                     /translation="VTMLAFGREALAYASVYFVTSATFTYTGGILLAASGRRSVRSAI
                     AGLVRIPAVYGAAAAAVVIGLGLPLPAAVQTPISLLGDAAVPMMILVLGMQLERAALP
                     RRPAVVATAVVISLVMTPLAALGIGRLVGLQGAAFQAGVLQASMPTAVITTILALEFD
                     ALPDFVTSVVFVSTLLSPFTVTLMIAYLKGL"
     gene            813..2525
                     /locus_tag="AC16CY_10215"
     CDS             813..2525
                     /locus_tag="AC16CY_10215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3354206.1"
                     /translation="MRAGLLAVTGVLCALLIFPDLFYWGRQGATGAQTSLALPDEVAS
                     LIQGRLTSDTGPELASLSPVEQARVRSLYESAGYAPRWVNRSGRLGGPARDGLTLLGK
                     AALDGLDPADYGRPRLGALATTLEAAHAPRAVDVATFDLAMSAGMLRYLAHLHRGRVD
                     PRATGLRLSRPPERLDVVMSLRSALEGGQITEMAERLTPPFAQYRRLREALARYRALA
                     GDTRFQSLPSVSRAVHAGEPYAGLVALYHRLVLLGDLSGDTPAPAPGAVYEAPLVEGV
                     KRFQARHGLEADGVIGHTTVAALLVPLDWRVRQIELALERLRWLPDLRDRRFVVLNIP
                     MFTLWAWDSLSASETPSLEMRAIVGRAGHTPTPVFSKPMHALIFRPYWNIPRSILRDE
                     ILPAVARDRGLLSRENMEIVRGAGDDAQPVDATDENLALLRQGSLRLRQRPGPVNALG
                     LVKFVFPNEENVHLHGTPAQRLFLRPRRDFSHGCVRVEDPVALAEWVLAGEPEWTRER
                     ILAAMSDIENVSQLVPLRRPIDVLLFYTTAMVPADAGAVRFAADIYGHDTRLDRALTR
                     GSAR"
     gene            complement(2624..3154)
                     /gene="mepK"
                     /locus_tag="AC16CY_10220"
     CDS             complement(2624..3154)
                     /gene="mepK"
                     /locus_tag="AC16CY_10220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan L,D-endopeptidase MepK"
                     /protein_id="HIR3354207.1"
                     /translation="MRTLSRRAFVATLATVPLAVPRRIHAGSAASRVLSFIHLHTGER
                     LVVEYFNRGSYLSDALGSINQFLRDFRTNEVHEIDQRLLDLLYGLNDVTGSRLPYQVI
                     SGYRSPATNQMLRQRSEGVAAGSLHMRGQAIDIRLPDVPLARLREAALSLRQGGVGYY
                     PASDFVHVDTGRVRTW"
     gene            3455..3889
                     /locus_tag="AC16CY_10225"
     CDS             3455..3889
                     /locus_tag="AC16CY_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR3354208.1"
                     /translation="MTTPQRDLAGNRASIRLWPGVVAAVLLWVLRFGVKAVVPGFTGF
                     SLGLLGAFAAAFVILVWWMVFSISLVLTPLAALGIGRLVGLQGAAFQAGVLQASMPTA
                     VITTILALEFDALPDFVTSVVLVSTLLSPFTVTLMIAYLKGL"
     gene            complement(3944..>4645)
                     /locus_tag="AC16CY_10230"
     CDS             complement(3944..>4645)
                     /locus_tag="AC16CY_10230"
                     /inference="COORDINATES: protein motif:HMM:NF047446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpL47-type beta-barrel domain-containing
                     protein"
                     /protein_id="HIR3354209.1"
                     /translation="PTQLDTNGNGVGDACDTSTPADTQPPVTTATLTPEPNAAGWLSI
                     QQAQVALNAIDDTSGVREIVYRLTGATVLASTAIPGTAAMVPIVAEGETIVRYFAVDN
                     AGNAGAEQSLTVRIDRTAPTVSCSAMPNVLSPPDRRLVTVSVAVVATDPLSGVSGFTL
                     VAATSDQPHDAAIQGWTIGTADTTGQLRAVLGTGGVARGYSLRYQVADRAGNRASCIA
                     NVSVPPLDQRRDSDR"
BASE COUNT          711 a         1540 c         1573 g          821 t
ORIGIN      
        1 ggtgacgatg ctcgcgttcg gccgcgaggc gctcgcgtac gccagcgtgt atttcgtgac
       61 gagcgcgacg ttcacctata cggggggaat ccttctggcc gcgagcggcc ggcgaagtgt
      121 acgcagcgcg atcgccggtc tggtcaggat tccggctgtc tacggagcgg cggccgcggc
      181 agtggtgatc ggcctggggc tccccctgcc ggcggccgtc cagacgccga tctcgctgct
      241 cggcgacgcg gccgtgccga tgatgattct cgtgctgggc atgcagctcg aacgtgcggc
      301 cctgcccaga cggccggcgg tcgttgcgac ggccgtcgtc atctcacttg tcatgacgcc
      361 gctcgccgca ctgggaatcg gccggctcgt cggcctgcag ggtgccgcgt tccaggccgg
      421 ggtgctgcag gcatcgatgc cgacggcggt gatcacgacg atcctcgccc tcgagttcga
      481 cgcgctgcca gacttcgtca ccagcgtcgt cttcgtctcg acgctgctca gccccttcac
      541 cgtcacactg atgatcgcgt atctgaaggg cctgtagacg cgtcgcccca agcgccacga
      601 cgccgacggc gggtgattcg acttttcatt caccgactcg agcaggtgcg caccggctag
      661 atcaaaggcc cgacagtgct gtctttacct gtacgggggg tccgcgcgtc cgatccagct
      721 tggcgcggag ggtgcatcga accttgagga gctcccccag gccgatccgt atgcggaaaa
      781 ctcggcaatc ggcgcctgtt ttctcccagg cggtgcgtgc aggccttctg gccgtcaccg
      841 gcgtgctgtg cgcgctgctc atcttccccg acctcttcta ctggggccgc cagggagcca
      901 ctggagctca gacgtcacta gcgcttcccg acgaggtcgc ctccctcatc caaggccggc
      961 tgacgagtga cactggtcca gagctcgcct cactgagccc ggtcgagcag gccagagtga
     1021 gaagcctgta cgagagcgcg ggatacgcgc ctcggtgggt taaccggtct ggccggctag
     1081 ggggtcccgc gcgagacggc ctgacgctgc tcggcaaagc cgcgcttgac ggtctcgatc
     1141 ccgctgacta cggccgcccc cggctcgggg ccttggcgac gacgctcgag gccgcgcacg
     1201 caccacgggc cgttgacgtc gccaccttcg atctcgccat gagcgccggc atgctgcggt
     1261 atctcgctca cctgcatcga ggacgcgtcg atccgcgggc gaccggcctt cggctgagcc
     1321 ggccaccaga gcgcctcgac gtcgtaatgt cgcttcgcag cgcgctggag ggcgggcaaa
     1381 tcaccgagat ggccgagagg ctcacgccac ctttcgcgca gtaccgccgg ctgcgcgagg
     1441 cgctggcccg gtaccgggcg cttgccggcg acacacgttt tcaatcactg ccgtcagtga
     1501 gccgagcggt acacgccggc gaaccctacg cggggcttgt cgctctctac caccgacttg
     1561 ttcttctcgg cgacctttcc ggcgatacac cagctcccgc gccgggcgcc gtgtacgagg
     1621 ctcctctcgt cgagggcgtg aagcgattcc aggcccgcca cgggctcgag gccgacgggg
     1681 tcatcgggca cacgacagtt gcggcgttgt tggtcccgct ggactggcgc gtgcgccaga
     1741 tcgagctcgc cctggagcgg ctgcggtggc ttcccgatct gcgtgatcgg cgtttcgtcg
     1801 ttctcaacat cccgatgttc acgctgtggg cgtgggactc actgtcggcg agcgagacgc
     1861 cgtcccttga gatgcgagcg atcgtgggcc gcgcgggcca caccccgacg cccgtgttca
     1921 gcaagccgat gcacgctctg atcttcaggc cctactggaa cattccgcgt tcgatccttc
     1981 gcgacgagat cctccctgcc gtcgcgcgcg acagaggcct cctcagccgg gagaacatgg
     2041 agatcgtgcg aggggccggc gatgacgcgc agccggtgga cgcgacagac gagaacctcg
     2101 cgctgctcag gcaaggctcg ctccgccttc ggcagcgccc tggaccggtc aacgcgctgg
     2161 gcctcgtcaa gttcgtgttc cccaacgaag agaacgtcca cctgcacggc acgccggcgc
     2221 agcggctgtt cctgcgaccc cgccgggact tcagccatgg ctgtgtgcgc gtggaggatc
     2281 cagtcgcgct ggcggagtgg gtcctcgcgg gcgagccgga atggaccaga gaacggatcc
     2341 tcgctgccat gtccgacatc gagaacgtct cgcaactggt accgctcagg cgcccgatcg
     2401 acgtgcttct cttctacacg acggcgatgg tgccagccga tgcgggcgcg gtccgtttcg
     2461 cggcggacat ctacgggcac gacacgcggc tggatagggc tctgacccgc gggtccgcgc
     2521 gttagggttc tgttgcggat tcactcgtgg agccagtctg gcgatctgac aatgacactc
     2581 atagacggct tcagccgccg cgtgagcctg gtctaccggg tgactaccag gtgcgtactc
     2641 gcccggtgtc gacgtggacg aagtcggacg cggggtagta gccgacgcct ccctgacgca
     2701 gggaaagcgc ggcctcgcga agtctggcga gcggcacgtc cgggaggcgg atgtcgatcg
     2761 cctgtccccg catgtgcagg ctgccggccg caacgccctc gctccgttgc ctgagcatct
     2821 ggttcgttgc tggagatcgg tacccggaaa tgacctgata gggcaggcgg ctgccggtca
     2881 cgtcattcag cccgtaaagc aggtcgagca ggcgctgatc gatctcgtgc acctcgttgg
     2941 tgcggaaatc ccggaggaac tgattgatcg agccaagcgc gtccgacagg tacgagccgc
     3001 ggttgaagta ttcgacgacg aggcgttcgc cggtgtgcag gtggatgaag ctcagcaccc
     3061 gactcgcggc ggatcccgca tggatccggc gcggtaccgc gagcggtacg gtggcgagcg
     3121 tggcgacgaa agcgcggcga gagagggtcc gcacgcagga aacgattata ggctacagcg
     3181 ctcgcattcg tcgtatcgtc gcctgttgcg gggccgcagc gactcgatca catggagcat
     3241 gcgtgaggac cgtcagcctc gacccgaacg cggccctttg cacacggcac atcccggcac
     3301 caagcggttc cacccgacag ccgctggtaa agggtcgcga ggctgatcgg tcgtcacgtc
     3361 aaccattgaa gacaaccacg ttgcgtatag caggcgcttc gttcggcgta aacgcgacgg
     3421 gaacccgccg cagccgacac cgtattggct tcatatgacg accccgcaac gggacctggc
     3481 gggcaatcgc gcatcgattc ggttgtggcc cggggttgtt gccgcggtgc tcctgtgggt
     3541 gttgcgcttt ggggtgaagg cggtcgtgcc cgggttcacg ggcttcagcc ttggcctcct
     3601 gggcgccttt gccgccgcgt ttgtcatcct cgtctggtgg atggtgttca gcatctctct
     3661 tgtcctcacg ccgctcgccg cactgggaat cggccggctc gtcggcttgc agggtgccgc
     3721 gttccaggcc ggggtgctgc aggcatcaat gccgacggcg gtgatcacga cgatcctcgc
     3781 cctcgagttc gacgcgctgc cagacttcgt caccagcgtc gttcttgttt cgacgctgct
     3841 cagcccgttc acggtcacgc tgatgattgc ctacctgaaa ggcctgtaga agccggaatc
     3901 aggaggccgc cgaccgagag cggcctccta accccagaga gcgtcagcga tcggaatcgc
     3961 ggcgctggtc gagcggcggc acgctgacat tcgcaatgca cgacgcccta ttgccagccc
     4021 ggtcggcgac ctggtagcgc agcgaatacc cgcgggctac ccctccagtc ccgaggacgg
     4081 cgcgcagctg tccagtggtg tcggcggtgc cgatcgtcca tccctggatc gcggcatcgt
     4141 gcggctgatc gctggtcgcg gcgacgagcg tgaagcccga gacgcccgac aggggatcgg
     4201 tggcgacgac ggcaacgctc acggtgacca gccggcgatc gggcggcgac aatacgtttg
     4261 gcatcgcgct gcaggacacc gtgggcgcag tgcgatcgat gcgaacggtc agcgactgct
     4321 cggccccggc gttgccggcg ttgtccacgg cgaagtagcg gacgatcgtc tcaccttcgg
     4381 cgacgatcgg caccatcgcc gctgttccgg ggatcgccgt ggacgcaagc accgtcgcac
     4441 cggtcaggcg atacacgatc tcgcggaccc cggaagtgtc gtcgatcgcg ttcaaggcga
     4501 cctgcgcctg ctggatcgac agccatcccg cggcattcgg ctcgggcgtc agcgtcgcgg
     4561 tcgtgaccgg gggctgtgtg tcggccggcg tgctcgtgtc gcacgcatcg ccgacgccgt
     4621 tgccgttggt gtccagttgg gtggg
//
LOCUS       DBNGSH010000444         6861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3776676, whole genome shotgun sequence.
ACCESSION   DBNGSH010000444 DBNGSH010000000
VERSION     DBNGSH010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3776676"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="AC16CY_10235"
     CDS             <1..798
                     /locus_tag="AC16CY_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR3354210.1"
                     /translation="GDGVNDAPALKKADVGIAMGIAGTDVSKEAAGMTLLDDNFATIV
                     AAVEEGRIVFGNIKKYLMYLLSCNVGEIVLLAGSVIAGFPLPLTAVQVLYVNLATDGL
                     PALALAVDPPERDLMRRKPRDPRVGIFTRPVVTMLLVAGLWSGLVNVTLFTTLLRNGR
                     PLEEVMALTFVSLVIIQFFNAYNCRSDRLSVFRNPFANRWLNLAVAWELLLLGVIIYV
                     PFFHRPFGTFSFAPADWLLTVVLAFSIVPVLEVVKWMARRGWFGELV"
     gene            818..1663
                     /locus_tag="AC16CY_10240"
     CDS             818..1663
                     /locus_tag="AC16CY_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354211.1"
                     /translation="MNSLTARSTIVAAGVFALVAALPPSLVAQWPSYPTAGIPRTADG
                     KPDLGAPAPRTADGKPDLSGIWNYAGILGFPGRGRPPDPPPGTPPEATFWNIELGMKE
                     GLPLRPWAAELRKQRMAVESKDNPDAACLPLGHMQLHTHSQPRKMVQTKDLIVIVYEA
                     NANVRQIFLDGRAAPPNDPQPWWYGYSRGWWEGDTLVVETTHFRDGGWLDVDGAPLTS
                     EGKLVERFRRPTFGRLEIDVTIDDPKAYTRPWTVRVNQRLLVDTELIEFVCLENERFS
                     SRLPK"
     gene            complement(1709..2125)
                     /locus_tag="AC16CY_10245"
     CDS             complement(1709..2125)
                     /locus_tag="AC16CY_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354212.1"
                     /translation="MRNSHSAAVPIHGFEDDAPERRARIEDSLADFASQAGLSRGVSL
                     PSLAAGTTVRVETQNSHYRIVVQDGRRGLVLIHGGRVFPEDTVANVEGATRGGSALRV
                     GWIGEGLRLELSTDHGRVVTSPVESVTVEDDAEDVH"
     gene            2300..3679
                     /locus_tag="AC16CY_10250"
     CDS             2300..3679
                     /locus_tag="AC16CY_10250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3354213.1"
                     /translation="MRAFVRFLVFLILVAGLVGGGAWFWAGRMDGPTIEIRQPGEFIG
                     QATMLELMVSSPGGEFSSVQVTVEQEGRSFEVFNLDQPAQGSLKQDTAERIYVMRPVG
                     KRAIGDLKEGQARIVVTASRPVLFGIRDAASTTTRDVKVRLQPPQAAVLSTFHYVNHG
                     GSEFVVYRAAPEDVQSGVRVGSIEYAGYPARAVGITSDPALRVAFFALLFDQDLNTPI
                     QVYARDPAGNETLVPFEYKVFPKPFSKSRIELDERFIGRVVPSIAAGSPDAQIPTDDV
                     LAGFLKINGDLRRKNNQYLTELARKSSPEKLFSAAFQQLGNSQVEARFADARTYVYQG
                     KEVDRQVHLGFDLAVTANVPIVAAERGTVVHASDLGIYGNCVVIDHGLGVQSLYGHLS
                     SIGVKVGDMVAKGAEIGRSGMTGLAGGDHLHFTMLVGGQQVTPVDWWSKQWYEDRVRR
                     KIVAAGGVG"
     gene            3767..4486
                     /locus_tag="AC16CY_10255"
     CDS             3767..4486
                     /locus_tag="AC16CY_10255"
                     /inference="COORDINATES: protein motif:HMM:NF026037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3354214.1"
                     /translation="MAASFAACSGSAPEESAPPPAPVANPVDATSAGSLTGSITLEGT
                     PPASETINRRSDSYCEGLGTATTQVFVVTNGGLQNVFVYIKDGLGGVKFPVPATPVVL
                     DQQGCTYLPRVFGIQAGQPLEIRNSDDTLHNIHALPRNNREFNRGQALKGIKHTHVFT
                     TAEVMVPFNCDVHKWMNAWVGVLDHPFHAVSGPGGAFEIQGVPPGTYTIEAWHEKLGT
                     QTQTVTIGPKEAKNLSFTFKI"
     gene            4512..5435
                     /gene="cyoE"
                     /locus_tag="AC16CY_10260"
     CDS             4512..5435
                     /gene="cyoE"
                     /locus_tag="AC16CY_10260"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR3354215.1"
                     /translation="MKTGTHVLDSPRIEGSAPPRALDRALDFLTLTKPRLNLLVLLTT
                     LAGLYMAAPDRVTLALAFHTLVGTALVAGGAAALNQVWERETDRKMRRTSGRPVPRGR
                     LRVAEGASFGIALSLAGLAELALGATAAAAVVAGLTLVSYVLVYTPLKTRTSLATLVG
                     AVPGALPPVIGWAAATGDVTLPALVLFGIVFFWQMPHFLAIAWMYKDDYANAGIPLLP
                     VIEPDGRRTGRQTVLYTAALWPVSLLPALVGLAGARYSAVATFLGILFFWLSVVFARN
                     RSAGTARRLFLFSITYLPLLLAALVADRLWL"
     gene            5426..5845
                     /locus_tag="AC16CY_10265"
     CDS             5426..5845
                     /locus_tag="AC16CY_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HIR3354216.1"
                     /translation="MALSISDLPALNATLNGLSAVCLVSGYAFIKRGDRLMHQRCMIA
                     ALVLSSLFLVSYVVYHANAGSRPFPGQGVIRPIYFLILITHVVLAAAVVPLALVTATR
                     GLRSQFDRHVRIARWTFPIWLYVSVTGVVIYLMLYQL"
     gene            6040..6696
                     /locus_tag="AC16CY_10270"
     CDS             6040..6696
                     /locus_tag="AC16CY_10270"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3354217.1"
                     /translation="MTRRLSIVVAAIMLIGSAVPAFAQGRAIGTVKDTAGKPLKGATV
                     RAVNPDMTPSQFTSTTDDKGRWVMLGLRVGTWTFVVEAPGFGTIQTEAPVRAGPMPQM
                     SFVLIRDPGPIPGSLEKTVIQDVAAANGLRDRGQYEQALNAYQQIRERNPKLTSVSLV
                     IASVYRKQASAEPDLATKRALLDKAITTYSQLLDDEAVGGRARAELETTRAEVQALSR
                     "
BASE COUNT         1137 a         2300 c         2246 g         1178 t
ORIGIN      
        1 ggagacggcg tcaacgatgc gccggccctc aaaaaggcgg acgtcggcat cgcgatgggc
       61 atcgcgggga ccgacgtaag caaggaagcg gcggggatga cgctgctcga cgacaacttc
      121 gcgaccattg tcgccgccgt cgaggaaggg cgcatcgtct tcgggaacat caagaagtat
      181 ctgatgtatc tgctctcctg caacgtcggc gagatcgtcc tcctggccgg ctccgtcatc
      241 gcagggtttc cgctgccgct gacggcggtg caggtgctct acgtgaatct cgcaaccgac
      301 ggcctgccgg cgctggcgct cgcggtcgat ccgccggaac gggacctgat gcggcggaag
      361 ccgagagacc cgcgcgttgg catcttcacg cggccggtgg tcacgatgct gctggtggcg
      421 gggctgtggt ccgggctggt gaacgtcacg ctgttcacga cgctgctgcg gaacgggcgg
      481 cccctcgaag aggtgatggc gttgacgttc gtgtcgctcg tcatcattca gttcttcaac
      541 gcctacaact gccgctccga tcgcctgtcg gtgtttcgaa acccgtttgc caaccgctgg
      601 ctcaacctgg ccgtggcctg ggaactgctg ctgctcggcg tcatcatcta cgtccccttc
      661 ttccaccgcc ccttcggcac gttcagcttc gcgccggcag actggctgct gaccgtcgtc
      721 ctggcgttct cgatcgtgcc cgtgctcgag gtggtgaaat ggatggcgcg gcgcggctgg
      781 tttggagagc tggtgtagac gataatttca gaccgtcgtg aactccctga cagcccggtc
      841 aacgattgtc gcagctggcg tgttcgcgct cgtcgcggcg ctgccgccct cgctcgtggc
      901 gcagtggccc tcgtatccca ccgcgggcat tccccgtacg gcggacggca agccggacct
      961 tggcgcgccc gcgccgcgca ccgccgacgg caaaccggac ctctccggca tctggaacta
     1021 cgctggcatc ctcggctttc caggccgcgg ccggccgccg gatccgccgc cgggcacgcc
     1081 acctgaagcc accttctgga acatcgagct cgggatgaag gaaggcctgc ccttgcgccc
     1141 gtgggcggcg gagcttcgca agcagcgcat ggccgtcgag agcaaggaca accccgatgc
     1201 ggcatgcctg ccgctcgggc acatgcagct gcacacgcac tcgcagccgc gcaagatggt
     1261 ccagacgaag gacctgatcg tgatcgtgta cgaggcgaac gccaacgtgc ggcagatctt
     1321 tctcgacgga cgcgccgcgc cccccaacga tccacagccg tggtggtacg gctactcgag
     1381 aggatggtgg gagggcgaca cgctggtggt cgagacgacc cacttcaggg acggaggctg
     1441 gctcgacgtg gacggcgccc cgctgacatc cgagggaaaa ctcgtcgagc gattccgccg
     1501 gcccaccttc ggcaggctgg aaatcgacgt gacgatcgac gatcccaagg cctatacccg
     1561 tccgtggacg gtacgggtca atcagcgtct gctcgtagac acggagctga tcgagtttgt
     1621 ctgtctcgag aacgaaagat tcagcagccg cctcccgaag taagcggctg ccccctgccc
     1681 cccgtcccct gccccctgtc ccctgtcgtt aatgcacgtc ctcggcgtcg tcttcgacgg
     1741 tgaccgactc gaccggcgag gtgacgacgc gaccgtgatc ggtcgagagc tcgagcctga
     1801 ggccttcgcc gatccaaccg accctcaatg cgctgccccc gcgcgtcgcg ccttcgacgt
     1861 tggccactgt gtcttccgga aacacgcggc cgccgtggat gagcaccaga ccgcgccgcc
     1921 cgtcctgcac gacgatccga tagtgcgagt tctgcgtctc gacccggacg gtggtacccg
     1981 ccgcgagcga agggagggat acgcctcgcg acaagccggc ctgggaggca aaatcggcga
     2041 gcgagtcctc gatccgcgcc cggcgttcgg gcgcatcgtc ctcgaacccg tgaatgggaa
     2101 cggcagcgct atgcgagttc cgcatggctt ggtacccccc tctgccgtag ataggtagca
     2161 acaggtctgc cagcgcaaaa atgggccatt tcgaccgggt ggcccgagaa gcactgctgt
     2221 ggggtggcaa cttttgcaca ctgcctcaac cgtggagggg cgaccggttc gtccgcgcgc
     2281 tcgcgctatc atgcgaaaca tgcgcgcttt cgtcaggttt ctcgtgttcc tcatcctcgt
     2341 tgccgggctc gtgggcggcg gtgcgtggtt ctgggccgga cgaatggacg gccccacgat
     2401 cgagattcgc cagccgggcg aattcatcgg ccaggcgacc atgctcgaac tgatggtgag
     2461 ctcgcccggt ggggagttct catcagtcca agtgacggtg gaacaggaag ggcggagctt
     2521 cgaggtcttc aacttggacc agcccgcgca aggcagcctg aagcaggaca ccgcggagcg
     2581 catctacgtc atgcgcccgg tgggcaaacg cgcgatcggc gacctgaaag aagggcaggc
     2641 gaggattgtc gtcaccgcct cgcggccggt gctcttcggg attcgagacg ccgcctcgac
     2701 gacgactcga gacgtgaagg tccggctcca gccgccgcag gcggccgtgc tctcgacgtt
     2761 ccattacgtc aatcacggcg gcagcgaatt cgtcgtctac cgggcggcgc ccgaagacgt
     2821 gcaatcgggt gtcagagtgg gcagcatcga gtacgccggt tatcccgcac gcgccgtcgg
     2881 cattacgtcc gaccccgcgc tgcgcgtcgc gttcttcgcg ctgctcttcg accaggatct
     2941 gaacacgccc atccaggtct acgcccgcga cccggcgggc aacgaaaccc tcgttccttt
     3001 cgagtacaag gtctttccga agccgttttc gaagagccgc atcgagctgg acgagcgctt
     3061 catcggcagg gtcgtgccat cgatcgcggc cggctcgccc gatgcgcaga tccccaccga
     3121 cgacgtgctg gcgggattcc tgaagatcaa tggcgacctg cggcgcaaga acaatcagta
     3181 cctcaccgag ctcgccagga agtcgtcacc cgagaagctc ttctcggcgg cgtttcagca
     3241 gcttggcaat tcgcaggtcg aggcgaggtt cgccgacgcg cgcacctacg tgtaccaagg
     3301 caaggaggtg gacaggcagg tgcacctcgg gttcgacctc gctgtcacgg ccaacgttcc
     3361 gatcgtggcc gctgaacgcg gcacggtcgt gcacgcgtcg gacctgggca tctacggcaa
     3421 ctgcgtggtg atcgatcacg gcctgggcgt gcagtcgctc tacggtcatc tctcgtcgat
     3481 cggagtaaag gtcggcgaca tggtggcgaa aggcgccgaa atcggccgaa gcgggatgac
     3541 cgggctggcc gggggcgacc acctgcactt cacgatgctc gtcggcggac agcaggtgac
     3601 gccggtggac tggtggagca agcagtggta cgaagaccgc gtccgcagga aaatcgtcgc
     3661 cgccgggggc gtgggataat cagggggcag gggtcagggg gcaggggtca gggctcgggc
     3721 ttccggttcc aggttctgca tgcgttccct tgccttcacg ctaatcttgg ccgcgtcctt
     3781 cgccgcctgc tcgggcagcg ctccggagga atccgcgccg ccgccggcgc cggtggccaa
     3841 ccctgttgac gcgacatcgg caggcagcct gacgggatcg atcacgctcg aaggcactcc
     3901 gccggcgtcg gagacaatca accgtcgatc ggactcctac tgcgagggcc tcggcacggc
     3961 cacgacccag gtcttcgtcg tgaccaacgg cgggctgcag aacgtgttcg tctacatcaa
     4021 ggatggcctc gggggggtga agtttcccgt ccccgcgaca cccgtggtgc tggaccaaca
     4081 gggctgcaca tatttgccgc gcgtcttcgg gatccaggcc ggtcagccgc tcgagattcg
     4141 caacagcgac gacacgctgc acaacatcca cgcgcttccg aggaacaacc gcgagttcaa
     4201 ccgtgggcag gcgctcaagg ggataaagca cactcacgtc ttcacaacag ccgaagtgat
     4261 ggtcccgttc aactgcgacg tgcacaagtg gatgaacgcg tgggtcgggg tgctcgacca
     4321 cccgttccat gccgtgagcg gtccgggcgg cgcgttcgag atccagggcg tgccgccggg
     4381 cacgtacacg atcgaggcgt ggcacgagaa gctcggcacg cagacgcaaa cggtgaccat
     4441 cggcccgaag gaggccaaga acctgtcctt caccttcaag atttagccag gatgatggca
     4501 agaggccgcg cgtgaagacg ggaacccacg ttcttgactc gccccggatc gaggggtctg
     4561 cccccccgcg ggcgctcgat cgggcgctcg acttcctcac gctcaccaag cccaggctga
     4621 acctgctcgt gctcctcacg acgctcgcgg gcctctacat ggccgcgccc gatcgcgtga
     4681 cgctcgcatt ggcgttccac acgctggtcg gaacagcgct cgtcgcgggc ggcgccgcgg
     4741 ccctgaacca ggtctgggag cgcgaaacgg accggaagat gcggcggacg agcggaaggc
     4801 ccgtaccgcg cggcaggctg cgcgtcgcag agggcgcgtc gttcggcatc gcgctgtcgc
     4861 tcgcggggct cgcggaactg gccctgggcg cgacggcggc ggccgcagtc gtcgcgggcc
     4921 tcacactcgt cagctacgtg ctcgtctaca cgcctctcaa gacgcgcacg tcgctggcca
     4981 cgctcgttgg cgccgtgccg ggggccttgc cgcccgtgat cggatgggcc gcggcgacgg
     5041 gcgacgtcac gctccccgcc ctcgtgctct tcggcatcgt gttcttctgg cagatgccgc
     5101 acttcctcgc catcgcgtgg atgtacaagg acgactacgc gaacgccggc attcccctcc
     5161 tgccagtcat cgaacccgac ggcaggcgca ccggccgcca gacggtcctc tacacggctg
     5221 cgctctggcc agtgagcctg ctgcccgccc tggtcggcct ggcgggagcg cgctacagcg
     5281 ccgtcgccac gttcctcggg atcctgttct tctggctgag cgtcgtcttt gcgcgcaacc
     5341 gatcggccgg caccgcgaga cgcttgttcc tcttctcgat cacgtacctg ccgttgctgc
     5401 tggctgccct cgtggcggat cggctgtggc tctgagcatc tcagaccttc ccgcgctcaa
     5461 cgccacgctg aacggactct cggcggtgtg tctcgtctcg gggtacgcgt tcatcaagcg
     5521 cggcgaccgc ctgatgcatc agcgatgcat gatcgcggcg ctcgttctgt cgagcctctt
     5581 cctcgtgagc tacgtcgtct accacgcaaa tgccggatcc cggccgttcc ccggtcaagg
     5641 agtcattcga ccgatctatt tcctgatcct gattacacac gtcgtgctcg cggcggccgt
     5701 tgtgcccctt gcgctcgtca cggcgacccg cggtttgcgg tcgcagttcg atcgacacgt
     5761 ccggattgcc cggtggacgt tcccgatctg gctctatgtc tcggtcacgg gcgtcgtgat
     5821 ctacctgatg ctctaccagc tctagtggac cgtatcaact gaacttgtag cgagtgggac
     5881 agccccgcga cgccgatttc gcgggccaac caacgggcgg cccgccctac atttcgttga
     5941 tacgctccac tagtgacgcc gagcctcacg ggaccttctg ggataagatc agacgctcca
     6001 cggggacggg cccgagacac ctcggcccgg gaggatcatg tgacgcgccg cttgtcgatc
     6061 gtcgtcgccg cgatcatgct gatcggctca gccgtaccgg cattcgccca ggggcgcgcc
     6121 atcggaacgg tcaaggacac cgcggggaag cccctcaagg gggcgacggt tcgcgcggtc
     6181 aacccagaca tgacgccgtc gcagttcacc tcgaccaccg acgacaaggg gcgctgggtc
     6241 atgctcggtc ttcgggtcgg cacgtggacg ttcgtcgtcg aggcgccggg attcgggacg
     6301 atccagacag aagccccggt ccgcgccggc cccatgcccc agatgagctt cgtgctgatc
     6361 cgcgatcccg gtccgattcc gggttcgctc gagaagaccg tgatccagga cgtggccgcg
     6421 gccaacgggc tccgcgatcg cggccagtac gagcaggcgc tgaacgccta tcagcagatt
     6481 cgcgagcgca atccaaagct gacatccgtg agcctcgtga tcgcgagcgt ctaccggaag
     6541 caggcgtcgg ccgagcccga cctcgccacg aagcgggcgc tgctcgacaa ggcgatcacc
     6601 acctacagcc agctgctcga cgacgaggcc gtcggcggcc gcgccagggc cgaactggaa
     6661 accacacgcg ctgaagtcca ggcgctgtcg cgctgacgat ggcgagccac gcgcgcggtc
     6721 aggctcgccg atggcgggca tcgatcatca tcgcgatgtt cttcgccgcc gtcgcaggcg
     6781 cggctgcggt cggatggtgg tatgcccgcg agtcgcctcc ccaccagggg ccgatcgtgg
     6841 tcctctcgat cgacggcgtg g
//
LOCUS       DBNGSH010000445         3206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3098122, whole genome shotgun sequence.
ACCESSION   DBNGSH010000445 DBNGSH010000000
VERSION     DBNGSH010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3098122"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..759
                     /locus_tag="AC16CY_10275"
     CDS             256..759
                     /locus_tag="AC16CY_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354218.1"
                     /translation="MRKFVMHVVTAAVLLVPSSLYAQDDPAFGLVTGYPANIGVIWHV
                     SERVAVRPDFNFTWFSTEGAFSDSDTTAIGIGISVLFYARATDQLRLYFVPRFGYGRT
                     STSTPNIDPTETTQGSGSFGAQYSLHRRFAVFGEAGVLFSHATGEFTTSSNSWGTRTG
                     VGVIVYF"
     gene            complement(768..2021)
                     /locus_tag="AC16CY_10280"
     CDS             complement(768..2021)
                     /locus_tag="AC16CY_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014135.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HIR3354219.1"
                     /translation="MPEINPALLSEARTLAAYLIFFGSYFVFALGKFPWMKIDRPGAA
                     IIGAVLMVALRIVGTQEALQSIDFATIVLLFSMMLVVANLRVGGFFERVAEWIIERLQ
                     PHHLLPTVIFTSGLLSAFLVNDIVCLVMTPFVLHVARRLGLPPVPYLVAVATASNIGS
                     AATITGNPQNMLIGSVSGIAYLDFIVHLGPVAVGGLILDWAVIHHLCLRGAVDRVTVA
                     PALAAPEFRHEPMRKKPVVVLALVLGGFLAGVPPALMAAVGAALLLITRTVEPRKVYD
                     EVDWGLLVFFVGLFVIVAGAERAGLTASLLQPIAAWDLHRLAIFVPFTAALSNLVSNV
                     PAVMLLRTLVDRFPDAHQGWLALSMASTLAGNLTITGSVANIIVVERAAAEGVRIGFR
                     EYFRIGLPVTAATLALGSVWLWISV"
     gene            complement(2055..2837)
                     /locus_tag="AC16CY_10285"
     CDS             complement(2055..2837)
                     /locus_tag="AC16CY_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354220.1"
                     /translation="MKPLAIAGAAILFSATLSAQWPLHPVGVPKGPDGKPNLSAPAPR
                     TPDGKPDLSGIWYPQGGRGFATLGNVAGGIPGGLPFQPWAAELQRTRAAADSWDNPDG
                     LCKPQGPLQYHTDPQPRKIVQTPTQILMIFESNYGLRTIYLDGRSLPPQGVPQPFWHG
                     YSVGRWEGDTLVVESNNFHDDGWLDARGSPYTEALKLTERIRRVNYGRLEIEVTIDDP
                     KAYTKPFTVRINQQIAADGQELIEFICHENQMFLKLTGREKR"
     gene            2931..>3206
                     /locus_tag="AC16CY_10290"
     CDS             2931..>3206
                     /locus_tag="AC16CY_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354221.1"
                     /translation="MRILMNRMTAVLALTLLVLAPVTNASAQLVAAKDGPIVYGHHHV
                     NATNVAEHMKFWVDALGGVKTTFGANKLEIVKFPNALLFMRAQAPKGG"
BASE COUNT          570 a         1075 c         1047 g          514 t
ORIGIN      
        1 gagacgatgt atcccgagta ccgcaagagg ctcaaggaca agtacacgcc gccgccgccc
       61 gtcgcgcccg caggacgggg caggggagcg ggagcaggag cggcgccgcc cgcaggggcc
      121 ccggcaggac ggggcggggg caagtaagca gcgcgaaccc gatcccggcg ttgctgaatt
      181 cctcgcaacg gattcgctca gccagtcgta tcagctgcag attgcggccg cctgccagcg
      241 ccgtggagat gtgtcatgcg gaagttcgtc atgcacgtgg tcaccgcagc ggtgctcctg
      301 gtaccctcgt cgctttacgc acaagacgac cccgccttcg gcctggtgac cggctatccg
      361 gccaacatcg gcgtcatctg gcacgtgtcg gaacgcgtgg ccgtccggcc ggacttcaac
      421 ttcacctggt tctcgaccga aggagccttc agcgattccg acacgaccgc aatcggaatc
      481 ggcatcagtg tcctgttcta cgcccgcgca acggatcaac tccgcttgta cttcgttcca
      541 cgattcggat acgggcggac atccacatcg actccgaaca tagaccccac tgaaacgacg
      601 caaggatccg gatcgttcgg cgcgcagtat tcattgcacc ggcgattcgc cgttttcggc
      661 gaggccggcg tgctcttcag ccacgcaacc ggagagttca ccacgagctc caactcgtgg
      721 ggcacgcgca ccggggttgg tgtcatcgtc tacttctgaa gatcgagtca taccgagatc
      781 cacagccaca cggatccgag cgcgagcgtc gccgccgtaa ccggcaggcc gattcggaag
      841 tactcccgga acccgatgcg cacgccctcg gccgccgcgc gctcgacgac gatgatgttg
      901 gcgaccgagc cggtgatcgt gaggttgccg gccagcgtcg aggccatgga aagcgccaac
      961 cagccctgat gtgcatcggg aaacctgtcc acgagcgttc gcagcagcat cacggccggg
     1021 acgttgctga ccaggttcga gagcgcggcc gtgaatggga cgaagatcgc gagccggtgc
     1081 aggtcccacg ccgcaatcgg ctgaaggagg ctggccgtca gccccgcgcg ctcggcgccc
     1141 gcgacgatca cgaaaagccc cacgaagaaa acgagcaggc cccagtccac ttcgtcgtag
     1201 accttccgtg gttccacggt ccgggtgatg aggagcagtg cggccccgac ggccgccatc
     1261 agggccggcg gcacgcccgc gaggaagcca ccgagcacga gggccagtac gacgaccggc
     1321 ttcttccgca tcggctcgtg ccggaactcg ggtgccgcca gcgccggcgc gacggtcacg
     1381 cgatcgacag ccccgcgcag acagaggtga tggatcacgg cccagtcgag gatcagcccg
     1441 cccacggcga ccggtcccag gtggacgatg aagtcgagat acgcgatccc ggacaccgac
     1501 ccgatcagca tgttctgcgg gttgccggtg atcgtcgccg cgctgccgat gttcgacgcg
     1561 gtggcgacgg cgacgaggta cggcacgggc ggcagcccca gccggcgcgc cacgtgcagc
     1621 acgaacggcg tcatcaccag gcagacgatg tcgtttacga gaaacgccga gagcaggccg
     1681 ctcgtgaaga tcaccgttgg cagcaggtgg tgcggctgca gccgctcgat gatccattcg
     1741 gcgacgcgct cgaagaagcc gccgacccgc aggttggcga cgacgagcat catcgagaac
     1801 agcagcacga tggtggcgaa gtcgatcgac tgcagggcct cctgcgtgcc gacgatgcgg
     1861 agcgcgacca tcagcacggc cccgatgatc gccgcgccag gccggtcgat cttcatccag
     1921 gggaacttgc cgagcgcgaa gacgaagtag ctgccgaaga agatcaggta ggcggcgagg
     1981 gtccgcgcct cggagaggag cgcggggttg atctcgggca tgttctcggc agcgggaacg
     2041 ccggcgacgc gccgctaccg tttttcccgg ccggtcagct tcaggaacat ctggttctcg
     2101 tggcagatga actcgatcag ctcctggccg tccgccgcga tctgctggtt gatccggacg
     2161 gtgaacggct tcgtgtacgc cttcgggtcg tcgatcgtga cttcgatctc gagccgcccg
     2221 tagttcacgc gccggatccg ctcggtcagc ttgagcgcct ccgtgtacgg gcttcctctc
     2281 gcgtcgagcc agccgtcgtc atggaagttg ttcgactcga cgaccagcgt gtcgccttcc
     2341 cagcgcccga cggagtagcc gtgccagaac ggctgcggga ctccctgggg cggcagcgag
     2401 cggccgtcca ggtagatcgt gcgaaggccg tagttcgact cgaagatcat gaggatctgc
     2461 gtcggcgtct ggacgatttt ccgcggctgc ggatcggtgt ggtattgcag cgggccctga
     2521 ggcttgcaca gcccgtccgg gttgtcccag ctgtcggccg ccgcgcgcgt ccgctggagc
     2581 tcggccgccc acggttggaa gggcagcccg cccggtattc cccctgcgac gttgccgagc
     2641 gtcgcgaaac ctctccctcc ctgcgggtac cagatcccag atagatccgg cttgccatcc
     2701 ggtgttcggg gcgccggcgc gctcagattg ggcttcccgt caggtccctt cggaacgccg
     2761 accggatgga gaggccactg cgccgacagc gtggccgaga agaggatcgc agcgccggcg
     2821 atggccaggg gcttcatgac gggaaactct accgcaattc ctgtattcac tcgcaccctc
     2881 gcaccctcgc accgtcgcac tctcgcaccc tcgcaccgta tactcccccg atgcgcattc
     2941 tgatgaaccg gatgactgcc gtccttgccc tgactcttct cgtgctcgcg cccgtgacca
     3001 acgcctcggc gcagctcgtc gctgccaagg acggaccgat cgtctacggc caccatcacg
     3061 tgaacgccac gaacgtcgcc gagcacatga agttctgggt ggacgccctt ggcggcgtca
     3121 agacgacgtt cggcgcgaac aagctcgaga tcgtcaagtt cccgaatgcg ctgctgttca
     3181 tgcgcgcgca ggcgcccaag ggcggc
//
LOCUS       DBNGSH010000446         4788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1908984, whole genome shotgun sequence.
ACCESSION   DBNGSH010000446 DBNGSH010000000
VERSION     DBNGSH010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1908984"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1888
                     /locus_tag="AC16CY_10295"
     CDS             80..1888
                     /locus_tag="AC16CY_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3354222.1"
                     /translation="MRALLAPALAAAVVALSASVALAQEPDGRSLFAQRCAACHTGEA
                     NSRAPGLDALAVRTPQAIVESLMTGAMRAQGSRMSGAERRAVAVFVTGKTIAGDVRGA
                     LTGRCTASAPLRDEAGGPSWSGWSPSAANTRFQLSTQAGLAAADLPRLKLRWAFGFPD
                     ASVAWAQPTFAGGRIFVGSQNGTVYSLDARTGCIRWTFSASGGVRTAIAIDHDSSRSR
                     FVAYFGDTAANVYAVDADTGQQVWVRSVDDHPLARITGSPTLHDGRLYVPVASYEESQ
                     GADPQYPCCTFRGSLAALDTRSGTIVWKTYMIDSPAARRGTSSAGVPLWGPSGAAIWS
                     APTVDATRGSLYVATGNAYTGPAPPTSDAVVALDLSTGAIRWTRQITPNDVYVSGCRA
                     GNPNCPETLGPDHDFGSPPVLARAGERDVIVIGQKSGVAFAMDPDRGGEVLWQYRAGQ
                     GGVLGGIEWGAAVDGERAYFAVSDITTPQPGGLHAVSLTTGQRAWYAPPRPPSCGSGR
                     GCNGAQSAAVTAIPGAIFSGSNDGSLRAYSSATGEILWEFDTNRSFTTVNGEPARGAS
                     MIGPGAIVAGGMVYVNSGYGAFGGRPGNVLLAFGLE"
     gene            1892..3250
                     /locus_tag="AC16CY_10300"
     CDS             1892..3250
                     /locus_tag="AC16CY_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3354223.1"
                     /translation="MRLHTCRALAFVAAIGVWSAAMPSVAVDAAQGPSARAAAPTFSR
                     DVAPIFYSKCVSCHRPGEVAPMSLITYRDVRPWAGAIREKVTSRVMPPWHADRQYGSF
                     RNDLSLTQREIDTIAAWVGAGASEGSPSELPALPKFPDGWQIGTPDKVFEMLTEYQVP
                     ASGTIDYQYFEVPTNFTEDRWMQAGEVRAGDRAHVHHIIVYVREPQPRARPAVVAIRP
                     ILPAGQAPAPAPARPPGEAETRGGRTVATGGSDAMLVNWAVGEDAPVFLPGTAKRIPA
                     GSTLVFQVHYTTNGTPGTDRSRLGVIFAKEPPAREIRTGAIANPRFAIPAGAANHEVE
                     AEATFSEDVKVWTMHPHMHLRGKDMTYTVVYPDGRNEIVLRVPKFDFGWQTDYWLSEP
                     LALPKGSKLHVRAHFDNSAANRANPDPAATVRWGDQTWEEMMIGFFTYTVEGPAPPRS
                     AP"
     gene            3399..4025
                     /locus_tag="AC16CY_10305"
     CDS             3399..4025
                     /locus_tag="AC16CY_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3354224.1"
                     /translation="MTQSVALLRGYPTARGVAGSIVRVTDEELVALARRGDTDAFDQL
                     VGRHQAAVYRAALAALRVPEDAEEAAQDAFVRAWSSLGRVRGDSSLRTWLLTIAWNRA
                     VSRRRTVSGWLRRKAPLDDAAGIAAAGGTPHDDLRARELRGRAARAIEALSPKLRDAL
                     LLTASGEYDYGEIAAMLKIPTGTLKWRVAEARKQVRARLAALGCIDAR"
     gene            4015..4572
                     /locus_tag="AC16CY_10310"
     CDS             4015..4572
                     /locus_tag="AC16CY_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354225.1"
                     /translation="MLVDHSDDLDALIDDTARSLSDVSPRASLREGVRVRIAGTREWR
                     RVPVWQPALTGALVVALVVLLWPRQRPDAPAGRIAPSIAPAGVRLRAASARQVGPGLA
                     EPAAAVESQVPATTANVESDAVSAVGERVMPPPVPGATIVAPLEIEPIAPELIVVEAI
                     SLPMPVALRPVEIERLTFQRRGELP"
     gene            4569..>4788
                     /locus_tag="AC16CY_10315"
     CDS             4569..>4788
                     /locus_tag="AC16CY_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354226.1"
                     /translation="MKRPLIAALVMAVVLAAGLVGAQVPPAPTAPPAAAAPAPAPRTA
                     PPAPAPRTVPPAPAPRPVPPPQAVTPRPA"
BASE COUNT          719 a         1656 c         1672 g          741 t
ORIGIN      
        1 ctgtccacgg tattcgagat cttccacgac gaagacgagg ccctgcggag cttttgcgtc
       61 ggattagaga gggcgtgacg tgcgcgcgct cctggcgccg gcgctcgcgg cagccgttgt
      121 cgccctcagc gcgagcgtcg cgctggcgca ggagcccgat ggccggtcgt tgttcgcgca
      181 gcgctgcgcg gcctgtcaca ccggcgaggc caactcacgc gcgccgggtc tcgacgcgct
      241 tgccgtccgc acaccccagg cgatcgtcga gagtctcatg accggcgcga tgcgcgcgca
      301 ggggtcgcgc atgagcggcg ccgagcggcg tgcggtcgcc gtgttcgtca ccggcaagac
      361 gatcgctggc gacgtccggg gcgccctcac cggacgctgc actgcgtcgg cgccccttcg
      421 cgacgaggcc ggcgggccct cctggagtgg atggagcccg tcggcggcca atacccgctt
      481 tcaactctcc acacaggcgg gacttgccgc ggctgacctg cctcgcctca agctcagatg
      541 ggcgttcggc tttccggacg cgtcggtcgc ctgggcgcag ccgacctttg ccggcggccg
      601 catcttcgtc ggcagccaga acggcaccgt gtactccctc gacgcacgaa caggctgcat
      661 tcgctggacg ttcagtgcct cgggaggagt ccgaaccgca atcgccatcg atcacgacag
      721 cagccggtcc cgattcgtcg cgtatttcgg cgacaccgcc gcgaacgtct acgccgtcga
      781 tgccgacacg gggcagcagg tctgggtccg ctccgtggac gatcatccgc tggcgcgaat
      841 caccggctcg ccgacgctgc acgatggccg actctacgtg ccggtggcgt cgtacgaaga
      901 gtcgcaaggc gcggacccgc agtacccgtg ctgcacgttc cgtggaagcc tcgcggcgct
      961 cgacacgcga tcgggaacga tcgtctggaa gacgtacatg atcgacagcc ccgccgcgcg
     1021 gcgtggaacg agcagtgcgg gtgtgcccct ctggggaccc tccggcgcgg ccatctggtc
     1081 ggcgccgacg gtcgatgcca cgcgcggatc gctctacgtg gctaccggca acgcatacac
     1141 cggcccggcg ccgccgacga gcgatgcggt cgtcgcgctc gatctctcga ccggcgccat
     1201 ccggtggacg cggcagatca cgccgaacga cgtctatgtg tcaggttgtc gcgccggcaa
     1261 ccccaactgc cctgagactc tcggtcccga ccacgacttc ggaagcccgc ccgtcctcgc
     1321 gcgggcgggg gagagggacg tgatcgtgat cgggcagaag tcgggcgttg ccttcgccat
     1381 ggacccggac cgcggcggcg aggtgctctg gcagtaccgc gcgggacaag ggggagtgct
     1441 tggcgggatc gagtgggggg cggcagtcga cggcgagcgt gcgtacttcg ccgtgtccga
     1501 catcacgacg ccgcagccag gcgggcttca cgcggtgagc ctgaccacgg gtcagcgggc
     1561 ctggtacgcg ccgcctcgcc cgccgtcgtg cggcagcggg cgcggatgca acggcgcgca
     1621 gtcggcggcg gtcaccgcga tccccggggc gatcttctcc gggtccaacg acggctcgct
     1681 ccgcgcgtac tcctcggcga caggcgagat tctatgggaa ttcgacacga accggtcgtt
     1741 caccaccgtc aacggcgagc cggccagggg cgcatcgatg atcggccccg gagcgattgt
     1801 ggctggtggt atggtgtatg tgaactcggg atacggcgcc ttcggcggaa gacctggtaa
     1861 cgtgctgctg gcctttggcc tggagtagct catgagactt cacacgtgtc gggcgctcgc
     1921 cttcgtcgcg gcgatcggtg tctggtccgc cgccatgccg tcagtcgcgg tggacgccgc
     1981 gcagggcccg tcggcccgcg ccgccgcgcc aaccttcagc cgcgacgtgg cgccgatctt
     2041 ttacagcaag tgcgtgagct gtcatcggcc tggcgaggtg gcccccatgt cgctcatcac
     2101 gtaccgcgac gtccgcccct gggccggggc gatccgcgag aaggtcacct cgcgcgtgat
     2161 gccgccgtgg catgcggatc gccagtacgg gagcttccgc aacgacctca gcctcacgca
     2221 gagggagatc gacacgatcg cggcgtgggt gggcgcaggg gcgagcgagg gcagtccatc
     2281 ggagttgccc gcgctgccga agttcccgga cggctggcag atcggaacgc ccgacaaggt
     2341 gttcgagatg ctgaccgagt accaggtgcc ggcgtcgggc acgatcgact accagtactt
     2401 cgaggtgccg accaatttca ccgaggaccg atggatgcag gccggtgaag tgcgcgccgg
     2461 cgatcgcgcg cacgtgcacc acatcatcgt ctacgtgagg gagccgcagc cgcgcgcgcg
     2521 gcccgccgtc gtcgccatca ggccgattct tccggccggc caggcgcccg cgccggctcc
     2581 ggcgcgcccg ccaggcgagg ccgagacccg cggcgggcgg accgtcgcga ccggcggttc
     2641 cgacgcaatg ctcgtcaact gggcggtggg cgaggatgct cccgtgttcc tgcccgggac
     2701 cgcgaagcgc atcccggctg gctcaacgct cgtgttccag gtgcactaca cgacgaacgg
     2761 aacgcccgga accgatcgat cgaggcttgg agtgattttc gcgaaggagc cgccggcacg
     2821 ggagatccgg acgggcgcga tcgccaatcc gcggtttgcg atccccgccg gggcggcgaa
     2881 ccacgaagtc gaggccgagg ccaccttcag cgaggacgtc aaggtctgga cgatgcaccc
     2941 gcacatgcac ctgcggggca aggacatgac ctacacggtt gtgtatcccg acggcaggaa
     3001 cgagatcgtg ctgcgtgtgc cgaagttcga tttcggatgg cagactgact actggctgtc
     3061 agagccgctc gcccttccga agggatccaa gctccacgtg cgcgcgcact tcgacaactc
     3121 ggctgccaat cgcgccaacc ccgatccagc ggcgaccgtg aggtggggcg atcagacgtg
     3181 ggaagagatg atgatcggct tcttcaccta cacggttgaa ggaccggcac cgccgcgttc
     3241 agcgccgtag ggagcgcatc gatccgatca cgaaccacgg agacacagag acacggagga
     3301 ttccgctttc atggttctcc gtgactccgt gtctccgtgg tttcgtcgtt gaaaatggac
     3361 gaggaattcc ctcgtcatat ccactcaacc tgacgcgaat gacccaatcg gtcgctctcc
     3421 tccgcggcta cccaaccgcc cgcggggtgg ccgggtctat tgtgcgtgtg accgacgagg
     3481 agctggtcgc cctcgcgcgc cgcggagaca cggatgcgtt cgaccaactc gtcggccgcc
     3541 atcaggcggc ggtctacagg gcggcgctcg cggctctgcg cgtgccagaa gacgccgagg
     3601 aggctgcgca ggacgcgttc gtgcgggcct ggtcgagcct gggccgagtc cgcggcgact
     3661 cctcgcttcg cacctggctg ctgacgatcg cctggaaccg cgcggtcagt cgccgccgga
     3721 ccgtgagcgg ctggctgcgg cggaaggcgc ctctcgatga cgccgcgggg atcgcggccg
     3781 ccggcgggac tccgcacgac gacctgcgcg cgcgtgagtt gaggggccgt gcggcacgag
     3841 ccatcgaggc gctgtcgcca aagctgcgcg atgcgctgtt gctcacggcg agcggagagt
     3901 acgactacgg cgagattgcg gcgatgctga aaattccgac gggcacgttg aaatggcgcg
     3961 tcgctgaagc gaggaagcag gtgcgggcgc gcctcgctgc gcttggatgc atcgatgctc
     4021 gttgatcact ctgatgacct cgacgcgctg atcgacgaca ccgcgcggtc gctctccgac
     4081 gtttcgccgc gcgcgtcgct gcgggaaggc gttcgagtcc ggatcgccgg aacgcgcgag
     4141 tggcggcgcg taccagtctg gcaaccggcg ctcacgggcg ctctcgtcgt ggcgctcgtc
     4201 gtactgctct ggcctcgcca aaggcccgac gcgccggccg gcagaatcgc gcccagcatc
     4261 gctccggctg gggtccgcct tcgcgcggct tcggcgagac aggtcggccc gggcctggcg
     4321 gaaccggccg ccgcggttga atcgcaagtc cccgcgacga ctgcgaatgt ggagagtgat
     4381 gccgtgagtg ccgtcgggga acgcgtgatg ccgcctcccg tgccgggggc cacgatcgtc
     4441 gcgccgctcg agatcgagcc gatcgccccg gaattgattg tggtcgaggc gatctctcta
     4501 ccgatgccgg tggccttgcg acccgtcgag atcgaacgcc ttaccttcca gcgaagagga
     4561 gagctcccat gaagcgcccg ctgattgctg cccttgtgat ggccgtcgtg cttgccgcag
     4621 gcctcgtggg cgcccaggtt ccacccgccc ccacggctcc gcccgccgcc gcggctcccg
     4681 cccccgcgcc tcgcacggcg ccgcctgccc ccgcgcctcg cacggtgccg cccgcccccg
     4741 cgcctcgccc ggtgcccccg ccgcaggccg tgacgcccag gcccgccc
//
LOCUS       DBNGSH010000447         3648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3267607, whole genome shotgun sequence.
ACCESSION   DBNGSH010000447 DBNGSH010000000
VERSION     DBNGSH010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3267607"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="AC16CY_10320"
     CDS             <1..744
                     /locus_tag="AC16CY_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3354227.1"
                     /translation="GSEIGLHGIDAWRDSNAGRTEMREIASITGRQKTGVRMHWLYFS
                     EQAPRQLEAAAFDYDSTWGYNDAVGFRAGTSQVFAFPGVPTLLELPLSIMDTALFAWD
                     RWAMTRADARRHCDVVLAHAAQTGGTVVVNWHCRSLAPERLWHSFYRELLDRLSGEGR
                     AWFATAGEAVEWFRWRRSISFQEASRDGTVRIRVAAASHSPMAGAIRVSLPVGGRMGF
                     DDRVFDGSGDIDIELPARALAASGSRATA"
     gene            762..1961
                     /locus_tag="AC16CY_10325"
     CDS             762..1961
                     /locus_tag="AC16CY_10325"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3354228.1"
                     /translation="MTQQTIPFLDLVSLHRDIEEELIAACRSAIRSAHFIGGAEVEGF
                     EREFAAYCGSKYCVGLSSGTDAVRFALMASGVRTGDAVITVPHTFIATVEGISQAGAS
                     TEFVDIDGRTYNMSPEALEAYLKACRIDAVSGRPLGQRTGKPIKAVVPVHLYGQPADM
                     DAINDIAGRYGLVVVEDACQAQGAEYVKGSSRRAGTLGKAAAFSFYPGKNLGACGEAG
                     AVTTDDEGVAAYIRMIRDHGQAQKYYHDIEGYNGRLDAIQAAFLRVKLKHLDQWNSQR
                     RAAAARYNELLAPLASRGGLVVPYEPEWSRAVYHLYVIRTADRDALGKHLRAENIHTG
                     LHYPVPLHQQKCYAAWGYRTGSFPVTERVAAEIVSLPMFPGLHDDQQHRVAQGLERFV
                     RLETAGR"
     gene            1999..3003
                     /locus_tag="AC16CY_10330"
     CDS             1999..3003
                     /locus_tag="AC16CY_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354229.1"
                     /translation="MNRGYADVQVRGKRVAVPCLRWGAHTVVVHGRWLRIASIHDEVL
                     AEGDVVSDPAQLISHMATNGLRADILTFSQKIYQQEPNHRHPFEWDNAAVADTTNFEK
                     WWEALPQESRKNVRRAARRDVSVRVATFDDAFASGVKAIYDETPIRQGRRFWHYGKAL
                     ETVKRENSTYSDRSEFLGAYCGDELIGFMKFVYVDRVAVMMQILSKAAHYDRRPMNAL
                     VAKAVEVCANKGIRYLMYSKFTFGNKRDSQIAEFKRRNGFVQIDYPRYYVPLTLRGKL
                     AVRLRLHRGLLGLLPPSIISLLSRVRASVLSAAIRGPRASGSSDASRLPLSSSSGESR
                     "
     gene            3025..3096
                     /locus_tag="AC16CY_10335"
     tRNA            3025..3096
                     /locus_tag="AC16CY_10335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3057..3059,aa:Thr,seq:tgt)
     gene            3317..>3648
                     /locus_tag="AC16CY_10340"
     CDS             3317..>3648
                     /locus_tag="AC16CY_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3354230.1"
                     /translation="MPYVSFTFDDFPRSALIEGGAILRRHDIRGTYFLSMQLLGRPSP
                     SGPIASSDDVKRLLDEGHELGCHTYEHLDGRHSSVEAFERSIQANRAALQVVVPGVEF
                     QVFAYPLNG"
BASE COUNT          646 a         1105 c         1219 g          678 t
ORIGIN      
        1 gggagcgaga tcggccttca cggtatcgat gcctggcgcg actccaacgc gggtcggacc
       61 gaaatgcggg agatcgcatc gattaccggc cgccagaaga cgggcgtcag gatgcactgg
      121 ttgtacttct cggaacaggc cccgcgacag ctggaggcgg ccgcgttcga ctacgactcg
      181 acgtggggct acaacgacgc cgtggggttt cgggccggga cgtcacaggt cttcgcgttc
      241 ccgggtgtgc cgacgctgct cgagctgccg ctctcgatca tggacacggc gctgtttgcg
      301 tgggatcgtt gggcgatgac gcgcgccgat gcacggcggc actgtgacgt ggtgctggcc
      361 cacgcggctc agaccggcgg caccgtggtc gtcaattggc actgccgcag cctcgcgccc
      421 gaacgcctct ggcactcgtt ctatcgcgag ctgctcgaca ggttgtcggg agagggccgc
      481 gcgtggttcg ccacggcggg agaagccgtc gaatggtttc gctggcggcg ctcgatctca
      541 ttccaggagg cctcgcgcga tgggaccgtg cggatccgtg tggcggcggc ttcacacagc
      601 ccgatggccg gagccatccg cgtgagcctg ccggtcggcg gccgcatggg attcgacgac
      661 cgcgtgttcg acggctcggg cgacatcgac atcgaactgc ctgctcgtgc gcttgccgca
      721 agcggctcgc gcgcgactgc ctaactccac gaagaggtga aatgactcaa cagacgattc
      781 cgtttctcga tctcgtgtcg ctccatcgag acatcgaaga ggagctcatc gcggcctgcc
      841 gatcggccat ccgctcggcc cacttcatcg gcggagcaga ggtcgagggc ttcgagcgcg
      901 aatttgcggc gtactgcggc agcaagtact gcgtcggtct gtcgagcggc accgacgcgg
      961 tgaggttcgc gctcatggcg tccggcgtgc ggacgggcga tgccgtgatc actgtgccgc
     1021 acaccttcat cgcgaccgtc gagggcatca gtcaggcggg ggccagcacc gagttcgtcg
     1081 atatcgatgg acgaacctac aacatgagcc ccgaggccct cgaggcctac ctgaaagcat
     1141 gccggatcga cgccgtcagc ggacggccac ttggccagcg gaccgggaag cctatcaagg
     1201 cagtcgttcc agtgcatctc tacggtcaac ctgccgacat ggacgcgatc aacgacatcg
     1261 ccgggcggta tggcctggtc gttgtcgagg atgcgtgcca ggctcaaggg gccgagtacg
     1321 tgaaaggctc gtcgcggcgc gccggcacgc tcggcaaagc ggcggcgttc agcttctatc
     1381 ccgggaagaa cctgggcgcg tgtggtgaag cgggggcggt gaccactgac gacgagggcg
     1441 tcgcggcgta catccgcatg atccgggatc acgggcaggc gcagaagtac taccacgaca
     1501 tcgagggcta caacggccgg ctcgacgcca tccaggcggc gttcctgcgc gtcaagttga
     1561 agcacctcga ccagtggaac tcccagcggc gggcagcggc cgcgcgctac aacgagctgc
     1621 tggcgccgct cgcgtcaaga ggcgggctcg tggtccctta cgagccggag tggagtcgcg
     1681 cggtctatca cctctacgtc atccggaccg ccgatcgcga tgcgctcggc aagcacctca
     1741 gggcagaaaa catccatacc gggttgcact atccggtgcc gctccatcag cagaaatgtt
     1801 atgccgcatg ggggtaccga acgggcagct tcccggtcac ggagcgggtc gcggccgaaa
     1861 tcgtctcgct cccgatgttt cccggcttgc atgacgatca gcagcaccgc gtcgcacagg
     1921 ggctcgaacg tttcgtgcgt ctggaaacgg ccggccgcta gcgtgggaga acggggaagt
     1981 ggagaacggg gaagtactgt gaaccgtggt tacgctgacg ttcaggtgcg gggcaagcgc
     2041 gtggccgtgc cgtgccttcg gtggggtgct cacactgttg tcgtccacgg acgatggctc
     2101 aggatagcca gcatccacga cgaagtgctc gctgaaggcg acgtcgtcag cgatcccgcc
     2161 cagttgatct cacacatggc gacgaacggt ctgcgagcgg acatcctgac gttctctcag
     2221 aagatctacc aacaggaacc gaaccaccgc catcccttcg agtgggacaa tgccgccgtt
     2281 gcagacacca cgaacttcga gaagtggtgg gaggcattgc cccaggagtc gagaaagaac
     2341 gtcagacggg ccgcgcggcg tgatgtatcc gtccgtgtcg ccaccttcga cgacgcgttc
     2401 gcgagcggcg tgaaggccat ctacgatgag acgccgattc gtcagggccg caggttctgg
     2461 cactacggca aggctctcga aacggtgaag cgggagaaca gcacctactc ggatcggagt
     2521 gagttcctgg gcgcgtactg tggagacgag ctcattggat tcatgaaatt cgtctacgtc
     2581 gaccgcgttg ccgtgatgat gcagattctg tcgaaggccg cgcattacga ccggcggccg
     2641 atgaacgcgc tggtagcgaa agccgtcgag gtgtgtgcga acaagggtat tcgctacctc
     2701 atgtacagca agttcacgtt cggcaacaag agggacagcc agatcgccga attcaagcgg
     2761 cgcaacggtt ttgtgcagat cgactatccg cgctactacg tgccgctcac gctgcgagga
     2821 aagctggcgg tcaggctgcg attgcaccga gggttgctcg ggctcttgcc gccctcgatt
     2881 atttcgctcc tgtcgcgcgt gcgggcgtct gtgctcagcg ccgcgattcg aggtccgcgt
     2941 gcgagcggct cctcagacgc gtcacgtctc ccgctgtcct cgtcgagtgg tgagtcgaga
     3001 tgagttcccc acgccaacct ttgtgccggt gtagctcaat ggtagagcgg ccgatttgta
     3061 atcggctgat ggagcgttcg aatcctcccg ccggctctca tacgtggcaa tggctgcagc
     3121 gcatttctga ggctttggtg gctggcatct tcggtgtaat cgatacggcg cccgtgggag
     3181 ctggaggtca acgggctgcg gtgttcaatc agcgcgagct cgcgtcgtga caaacgtgat
     3241 tcgccggctt tcggggaaat ggcgacgctt cagcactgag tcgttcggcc gtcgcgactt
     3301 caagctctcg acggatgtgc cgtacgtgtc gtttacgttc gatgactttc ctcgctctgc
     3361 cttgatcgag ggtggggcga ttctgcgtcg gcacgatatc cgcggcacat actttctctc
     3421 gatgcagctg ctcgggcggc ccagtccttc cggtcctatc gcgtcatccg acgacgtgaa
     3481 gaggctcctc gacgagggtc atgagctggg atgccacacc tacgagcacc tcgacggccg
     3541 tcactcgagc gtcgaggcgt tcgagcggtc gattcaggcc aatcgcgccg cgctccaagt
     3601 tgtagtgcct ggcgtcgagt ttcaggtgtt cgcctatccg ctgaatgg
//
LOCUS       DBNGSH010000448         4867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4456169, whole genome shotgun sequence.
ACCESSION   DBNGSH010000448 DBNGSH010000000
VERSION     DBNGSH010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4456169"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /gene="ligA"
                     /locus_tag="AC16CY_10345"
     CDS             <1..1625
                     /gene="ligA"
                     /locus_tag="AC16CY_10345"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR3354231.1"
                     /translation="KLAQPVPGAIEVRGEVYLPRRAFEKMNRERMDADEPLFANPRNA
                     AAGALRNLDPALVARRGLQAFVYQLILGPAQTRLGSHAGTLDQLSRWALPVEPHWKKC
                     AGIDAVASFCQEWAEKRRDLGFDTDGVVIKLDSLDERRRLGSTSKFPRWAIAFKFPAE
                     QKTTLLKAIEVNVGRTGAVTPFAVLEPVFVGGTTVSMATLHNADDIARKDIREGDWVI
                     VEKAGEVIPRVVGPVVSRRPADSRPWIMPATCPRCGSQLHRAEEEAVVRCENVSCPAK
                     LQRGLEHFASRGAMNIEGLGESLVSQLIGSGLVRDYADLYSLTTDQLAALTSTSERGD
                     GKMIERRVGEKNAGKVVEQIARSRTNEFWRVIYALGIRHIGERGAQVLARAFGTMEAL
                     EAASREALQSTHEIGPVLAESMRTWLDEPRNRTLVQRLKAAGVRMEVPESERALAARP
                     QPFAGRTYVITGTLASMTREEATGALERLGAKVAGSVSKKTTAVIVGEEAGSKADKAR
                     ALGVPMLDEAAFRALIEQASGPGPQASDGTEA"
     gene            1657..3144
                     /locus_tag="AC16CY_10350"
     CDS             1657..3144
                     /locus_tag="AC16CY_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3354232.1"
                     /translation="MPRRFLIVTMCLIGTVAFLIGLIVAGSLTPTPAHSAPERKGAVV
                     RPAKTSPAGPALASFADVADRINPAVVNIDATTRGSYRDRSPSAAPRSETPGQFGRAP
                     ERERQGPRRGAGAGFIIDPDGYILTNHHLVEDAERLTVRLADGRSLRAEAVGSDPDTD
                     IALIKVSAPRPLPYATLGDSDTLRVGDWVLAIGNPLAYEHTVTAGVVSFIGRKLFDSS
                     LDRYIQTDAAIGFGSSGGPLVNSRGEVIGINAAISSRASNIGFAVPINQATEILPQLR
                     EAGRVTRGYIGVGLRDVDPDLQRSLGLKSSHGAMVVDVRDGSPGARAGLRPYDVIVAV
                     DGKPVGGNDELIQVVASGQPGTTATLNVVRDGRALNVVVKLAERASVRESEGQPSDFS
                     LPVRRPGSLLGLSVREIDAEFAERYDLPKDMGGVLVARVDPVGPAIDADIERGQVLLE
                     INRRPVRSVDDFRRLTANARPGDVLALYLFKPEISRRELRTVRVD"
     gene            3182..4585
                     /locus_tag="AC16CY_10355"
     CDS             3182..4585
                     /locus_tag="AC16CY_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3354233.1"
                     /translation="MKSRILVIDDEPAIRDSLRMILEYEDYQFVGAASGQEALDVLHR
                     ERPDVVLLDIKMPGVDGMEVLRKLRAIDETLPVIMISGHGTTATAVEAIRSGAIDFLD
                     KPLSSERVIVTLRNVLRQSELTTENRDLRVAMEAKYELVGQSPALRRVLEAVTRAAPT
                     NATVLLLGESGVGKELVARTIHRNSPRASQRFVQVNCAAIPEELIESELFGHEKGSFT
                     GATEKQVGKFEQADRGTIFLDEIGDMSQKTQAKVLRVLQEQEVERLGSAKTIKVDVRV
                     IAATNKDLEGAIGRGEFREDLYFRLNVIPIVVPPLRDRREDIPKLVQHFAKRMAEEHN
                     LKAKRIEPAAMDALQRHRWRGNIRELRNTVERLLIMTPSDSIRVDDLPGEIRVADAGP
                     RAPADAEGAARAADSAPAGAGTLREFKDASERAYLVQKLRETNWNISRTAEIIDTPRS
                     NLYKKLEQYGIKQETDG"
BASE COUNT          873 a         1583 c         1700 g          711 t
ORIGIN      
        1 tgaaactggc tcagcccgtg ccgggagcga tcgaggtgcg cggcgaggtc tatctgccgc
       61 gacgggcgtt cgagaagatg aaccgcgagc gcatggacgc cgacgagccg ctctttgcga
      121 atccgcgcaa cgccgcggcg ggagcgctga ggaatctcga ccccgccctc gtggcgcggc
      181 gcgggctgca ggcgttcgtg taccagttga tcctcggtcc cgcccagacc cgcctgggat
      241 cacacgccgg tacgctcgat cagttgagcc gatgggccct cccagtggag ccacactgga
      301 agaaatgcgc gggcatcgat gcagttgcat cgttttgcca ggagtgggcg gaaaagcgaa
      361 gggacctcgg attcgacacc gatggcgtcg tcatcaagct cgattcgctc gacgagcggc
      421 ggcggctggg cagcacgagc aagtttccgc gctgggcaat cgcattcaag ttccccgccg
      481 agcagaagac gaccctgctc aaggcgatcg aggtcaacgt tggccgcacc ggcgcagtga
      541 ctccatttgc cgtgctcgaa ccggtattcg tcggcggcac gacggtgtcg atggcgacgc
      601 tgcacaacgc cgacgacatc gcacgcaagg atattcgcga aggcgattgg gtgattgtcg
      661 aaaaggctgg tgaggtcatt ccgcgcgttg tgggccccgt cgtgtcgagg cggcccgcgg
      721 attccaggcc atggatcatg cccgccacct gtccgaggtg cggcagccag ctgcaccggg
      781 cggaggagga ggccgtcgtc cgctgcgaga acgtgtcgtg cccggcgaag ctgcagcgcg
      841 gtctcgagca cttcgcctca cggggagcga tgaacatcga agggctcggg gaatcgctcg
      901 tttctcaact catcgggagc ggcctcgtgc gggactatgc ggatctctac agcctgacga
      961 cggatcagct cgcggccctc acgagcacct cggaacgcgg cgacggcaag atgatcgagc
     1021 gccgcgtggg cgagaagaac gccgggaaag tcgtcgagca gatcgcgcgc agcaggacga
     1081 acgagttctg gcgcgtgatc tatgccctcg gcatccgcca catcggcgag cggggggcac
     1141 aggtgctggc gcgggcgttc ggaacgatgg aagcgctcga agcggcctcc cgcgaggcgc
     1201 tccaaagcac gcacgagatc ggcccggtcc tggccgaatc catgcgcacg tggctcgacg
     1261 agccgcgaaa ccggacgctc gtccagcggt tgaaggcggc gggggtccgc atggaggttc
     1321 cggaatcgga acgggcgctg gcggcgcggc cccagccgtt tgccggccgg acctacgtca
     1381 tcaccgggac gctggcgtcg atgacgcgcg aagaggcgac cggtgcactc gagcgtctcg
     1441 gggcgaaggt cgccgggtcg gtcagcaaga agacgacggc cgtcatcgtc ggcgaagaag
     1501 cggggagcaa ggccgacaag gcgagggccc tgggcgttcc gatgctggac gaggcggctt
     1561 ttcgggctct gatagaacag gcgtcagggc ccgggcccca ggcctcagat ggcactgagg
     1621 cctgaggcct gagccctggg gcctataatg atctcgatgc ctcgacggtt tctgatcgtg
     1681 acgatgtgcc tcatcggcac cgtcgcgttc ctcatcggct tgatcgtcgc cgggtcgctg
     1741 acgccgacgc cggcgcactc ggctcccgag cgcaaaggag cggtcgtccg gcccgcgaag
     1801 acgtcacctg ccggccccgc gctcgcctcc ttcgccgacg ttgcggaccg catcaacccc
     1861 gccgtcgtga acatcgacgc gaccacgcga ggatcgtacc gggatcgatc cccctccgcc
     1921 gcgccacgat cggagacccc gggccagttc ggccgcgcac ccgagcgcga gcggcagggg
     1981 ccgcgccgcg gcgctggcgc tggcttcatc atcgatcctg acgggtacat cctgacgaac
     2041 catcacctcg tcgaggacgc ggagcgcctg accgttcgcc tggccgacgg ccggagcctc
     2101 agagccgaag cggtcggctc ggaccccgac accgacatcg cgctcatcaa ggtatccgcg
     2161 ccgcgtccgc tgccgtacgc gacgctcggc gattccgaca cgttgcgcgt gggggactgg
     2221 gtgcttgcga ttggcaaccc cctcgcgtac gagcacaccg tcaccgccgg cgtcgtcagc
     2281 ttcatcggac ggaagctgtt cgactcgtcg ctcgaccgct acatacagac ggatgccgcg
     2341 atcggcttcg gcagcagcgg cgggccgctc gtcaattccc gcggcgaggt gatcggcatc
     2401 aacgcggcga tcagctcgcg ggcgtccaac atcggcttcg cggttccgat caatcaggcc
     2461 accgagatcc ttccgcagtt gcgcgaagcc ggacgggtca cccgcggcta catcggcgtg
     2521 gggctgcgcg acgtcgaccc ggatctgcag cgatcgctcg ggttgaaatc gtcacacggc
     2581 gcgatggtcg tggacgtcag agacggctcg cccggcgccc gagcaggact tcggccctac
     2641 gacgtgatcg tggccgttga cggcaagcct gttggcggca acgacgagct gatccaggtg
     2701 gtggcgtcgg ggcaacccgg aacgacggcg acgctcaacg tcgtccgaga cggccgggcg
     2761 ctcaacgtcg tcgtgaagct ggccgaacgg gcgtcggtcc gcgaatcgga ggggcagcca
     2821 tcggacttct cgctgccagt ccgcaggccc gggtcccttc tgggcctgtc agtccgcgaa
     2881 atcgacgccg agttcgccga gcgctacgac ctgcccaagg acatgggcgg cgtcctcgtc
     2941 gccagggtcg atccggtggg accggcgatc gacgcggaca tcgagcgcgg gcaggtcctg
     3001 ctcgagatca atcgccgccc ggtccgttcc gtggacgatt tccggcgcct gacggccaac
     3061 gcgcggcccg gcgacgtgct ggcgctctat ctgttcaagc ccgagatctc gcggcgcgag
     3121 ctgcgcacgg tgagggtcga ctagaccgtt ccgtgttccc aggaaccgga acagtccgtt
     3181 catgaagtcc cgcatcctgg tcatcgacga cgagccggcc attcgcgatt cgctgcggat
     3241 gattctggag tacgaggact accagttcgt cggcgccgcc agcggacagg aggcgctgga
     3301 cgtcctgcac cgggagcggc ccgacgtcgt gctcctcgac atcaagatgc caggcgttga
     3361 cggcatggaa gtgctgcgga agctgcgtgc gatcgacgag acgctgcccg tgatcatgat
     3421 ctccggccac gggacgaccg ccacggcagt cgaagcgatc cgctccggcg ccatcgactt
     3481 tctcgacaag cctctgagca gcgagcgcgt catcgtcacg ctgcggaacg tcctccggca
     3541 gtcggaactg acgacggaga accgcgacct gcgcgtggcg atggaggcga agtacgagct
     3601 ggtcgggcag agcccggcgc tgcgccgcgt cctggaggcg gtcacgcgcg ccgcgccgac
     3661 caacgcgacg gtgctgctgc tcggcgagag tggcgtcggc aaggagctgg tggcacgcac
     3721 cattcaccgg aacagcccgc gggcgagcca gcgcttcgtc caggtcaatt gcgccgccat
     3781 cccggaggag ctgatcgaat cggaactctt cggccacgag aaaggctcgt tcacgggggc
     3841 gaccgagaaa caggtcggca agttcgagca ggccgaccgc gggacgatct tcctcgacga
     3901 gatcggcgac atgagccaga agacgcaggc gaaagtgctg cgtgtgcttc aggagcagga
     3961 ggtcgagcgg ctcggatcgg cgaagacaat caaagtggac gtccgcgtca tcgcggcgac
     4021 gaacaaggac ctcgaggggg cgatcggccg gggagagttc cgtgaggact tgtacttccg
     4081 gctgaacgtc atcccgatcg tcgtgccgcc gctgcgcgat cggcgcgagg acattccgaa
     4141 gctcgtgcag catttcgcga aacggatggc cgaggagcac aacctgaagg ccaagcggat
     4201 cgagccggcg gcgatggacg cgttgcagcg ccatcgctgg cgcgggaaca tcagggagct
     4261 gcgcaacacc gtcgaacggc tgctcatcat gacgccgtcg gattccatcc gtgttgacga
     4321 cttgcccggg gagattcgcg tcgccgatgc cgggccgcgc gcgcccgccg acgccgaagg
     4381 tgccgccagg gcggccgact cggcgccggc cggtgcgggg acgctccgcg aattcaagga
     4441 cgcctccgag cgcgcctatc tcgtgcagaa gctgcgcgag acgaactgga acatctcacg
     4501 gacggcggag atcatcgata cgccccgcag caacctgtac aagaagctcg agcagtacgg
     4561 gatcaaacag gagacagacg ggtagaggat ccgcgcacgc tgaagcgtgc gctctacaga
     4621 cccgccgcgc ggcggcttca gccgccgcgt gaacgacatg cgacaataga ggcgtcgcag
     4681 tcagccaaga cgaccgccgg ccttcgcacc cggatttcag cgggctcgcc tgctgaggcc
     4741 cgaaggacgg aggcgggagg aaagtccgaa ctccgaaggg cagtgtgccg ggtaacgccc
     4801 gggcagggca acctgacgga cagtggcaca gaaaacatac cgccccgagc gaggcgcgtc
     4861 cgcaagg
//
LOCUS       DBNGSH010000449         3431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_381580, whole genome shotgun sequence.
ACCESSION   DBNGSH010000449 DBNGSH010000000
VERSION     DBNGSH010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_381580"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="AC16CY_10360"
     CDS             complement(<1..545)
                     /locus_tag="AC16CY_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3354234.1"
                     /translation="MAIPRLPVVALVVLSTAPAWGQSVRDHTRLAPEHRTKVTEAQAT
                     ELTLTVTPVAVRPVQVWLRTAGAIDAARRIVRAEVPAADGRRLRAGQRVRAFSPESRA
                     RMHQGTVSQVVRRAATAAFTVSLMGTALETTRHYVLEVVTDEGDFLSVPNEAIIDTGG
                     RTIVYVQESGGGYVSRDIKPGV"
     gene            complement(602..1801)
                     /locus_tag="AC16CY_10365"
     CDS             complement(602..1801)
                     /locus_tag="AC16CY_10365"
                     /inference="COORDINATES: protein motif:HMM:NF044870.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3354235.1"
                     /translation="MSTPASLRTFVIGGAITVALLASIVAGLKAQDVKITDPRSVVMI
                     VNRDSNDIGFLDIKTKKMIGRTFLGNNVNPHMVMMSPDGRYVVTGGTRANKAFIIDTR
                     TLQLVKVIPTDIAPEHLAFSPDSRWYYQGNPDGDSVSVIDMQSLSKIKTIPGFAEPLN
                     VTFTPDGTKAYVGNYGAHWVGVIDVRRHELLKKIQIAPVPAVAKLDPAKYLGEIKGIN
                     IAAPSNDGRYLYAADSDLAIVGVIDTREDRVIKTIRVGRDPWRIYMSHDGKYGITANN
                     GDETISIIDTASNTVAATLEAGPDMTGINFAAGKAFVISSTSGFVYVYDMKSLTSAGR
                     IKIGTNINLETATTDTADEKLYLAESTNHEVVIIDARTHALERVRNVGLFPWGTHIMD
                     SKDNYCH"
     gene            complement(1811..>3431)
                     /locus_tag="AC16CY_10370"
     CDS             complement(1811..>3431)
                     /locus_tag="AC16CY_10370"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3354236.1"
                     /translation="GWRKRLARGTGAAMLVFWCGSAAAQTPAAQAPMNMADHRGGIRG
                     VVRNDAGAPVAEAAVTAVNAENGAKFTATSDAQGAYSFGALPVGRYDITITSAGQTAF
                     RRAGVAVEMDRTAPLDITLNAASAAAAAEFERQQLLERIATLEQRITDLESNTVLSDP
                     ETRVRRVEKFVDASGNETDTQVPGSTPVVTYERERVYRRQTINEKIEEALEEAAEHSV
                     SVGVDAAVSTQFANLTQGTMDPTSGAYALASADLFFTAGIAQYTVFFADIVGLSGSPP
                     DTEIPTLSLLNGYTARLVAQNQLNLREAWLRTELFGQRLALTAGRLDLTNYFDQNALA
                     NDESTQFLSDALVNNQMLGLSSNGTGVAAEYDTKRGFRVKFGFQQSNDEASNLSDSIY
                     TLSEAGYTFTPFTLPEGTYRVWFRTDNTTDEITHGAGFSIDQKISPALGIFARYGTSA
                     LTVGRDNFYSVGVGISNGLVFNPQDTWGVGYAQMDLESGEAEKLVEGYYNFMLTEKLR
                     LSLHLTGVLDHPDTDSEFAYVLPSIRFQAAF"
BASE COUNT          516 a         1093 c         1152 g          670 t
ORIGIN      
        1 acgcccggct tgatgtcgcg cgagacgtaa ccgccgccgc tctcctggac gtacacgatc
       61 gtcctgcctc ccgtgtcgat gatcgcctcg tttggcacgg agagaaagtc cccttcgtca
      121 gtgacgactt cgaggacgta atgccgcgtc gtttcgaggg ccgtgcccat cagcgagacc
      181 gtgaaggcgg cggtggcggc ccggcggacg acctgcgaga ccgttccctg gtgcatgcgc
      241 gcgcgcgact cgggcgagaa ggcccgcacg cgctggccgg cccggagacg ccggccgtcc
      301 gcggccggca cctcggcccg gacgatcctg cgggcagcat cgatggcacc tgccgtcctg
      361 agccacacct gcaccggcct gacggcgacc ggcgtcacgg tcagcgtgag ctcggtggcc
      421 tgcgcctcgg tgaccttcgt cctgtgctcg ggcgcgaggc gcgtatggtc gcgcacactc
      481 tggccccagg ctggtgccgt ggagagcacg accagtgcga cgacgggaag tcggggaatc
      541 gccactcgct agtcttgagt tttgagtctt gagttttgag ttttgagtta cctggcgtct
      601 cctagtggca gtaattgtcc ttgctgtcca tgatgtgggt gccccacggg aagaggccga
      661 cgttgcggac gcgctcgagc gcgtgcgtcc tggcgtcgat gatcaccacc tcgtggttgg
      721 tggactcggc gaggtagagc ttctcgtcag ccgtgtcggt ggtcgccgtt tccaggttga
      781 tgttggtgcc gatcttgatg cgccccgccg acgtcaggct cttcatgtcg tagacgtaga
      841 cgaagccgct cgtggagctg atgacgaacg ccttgccggc cgcgaaattg atgccggtca
      901 tgtccgggcc cgcttcgagc gtcgcggcga cggtgttgct ggcggtgtcg atgatcgaga
      961 tcgtctcgtc cccgttgttg gccgtgatgc cgtacttgcc gtcgtggctc atgtagatgc
     1021 gccatggatc gcgcccgacg cgaatcgtct tgatcaccct gtcctcgcgc gtgtcgatca
     1081 cgccgacgat cgccagatcg ctgtcggcgg cgtagaggta gcggccgtcg ttggacggcg
     1141 cggcgatgtt gatccccttg atctcgccga ggtacttcgc cggatcgagc ttggccaccg
     1201 cgggcaccgg cgcgatctgg atcttcttca gcagctcgtg gcggcggacg tcgatgacgc
     1261 cgacccagtg ggccccgtag ttgccgacat acgccttcgt gccgtccggc gtgaacgtca
     1321 cgttcagcgg ctcggcgaag ccggggatcg tcttgatctt gctgagcgac tgcatgtcga
     1381 tgaccgacac cgagtctccg tcggggttcc cctggtagta ccacctgctg tcgggcgaaa
     1441 acgccaggtg ctccggcgcg atgtcggtcg gaatgacctt gacgagctgc agcgtgcgcg
     1501 tgtcgatgat gaaggccttg ttggcgcgcg tcccgccggt cacgacgtac cggccgtcgg
     1561 gcgacatcat caccatgtgc gggttgacgt tgttgcccag gaacgtccgg ccgatcatct
     1621 tcttggtctt gatgtccagg aatccgatgt cgttcgagtc gcggttcacg atcatgacga
     1681 ccgatcgcgg atcggtgatc ttgacgtcct gcgccttcag gcccgcgacg atcgatgcga
     1741 gaagggcaac cgtgatcgct ccaccaatga cgaacgtgcg cagggatgca ggcgtagaca
     1801 tgacggtctc ctagaacgcg gcctggaagc ggatggacgg caagacgtac gcgaactcac
     1861 tgtcggtgtc cgggtggtcg aggacgcccg tgaggtgcag cgagaggcgc agcttctccg
     1921 tcagcatgaa attgtagtag ccctcgacca gcttctcggc ctcgcccgac tcgagatcca
     1981 tctgcgcgta gcccacgccc cacgtatcct gcgggttgaa tacgagcccg ttggagatgc
     2041 ccaccccgac gctgtagaag ttgtcccggc cgaccgtcaa cgccgacgtg ccgtagcgcg
     2101 cgaatatgcc gagcgccggt gagatcttct gatcgatgct gaaccccgcg ccgtgggtga
     2161 tctcgtcggt ggtgttgtcg gtccggaacc agacgcggta ggtgccctcg ggcagggtaa
     2221 acggcgtgaa cgtgtagccc gcctcgctca gcgtgtagat tgagtccgac aggttgctcg
     2281 cctcgtcgtt gctctgctgg aagccgaact tgacgcggaa gccgcgcttg gtgtcgtact
     2341 cggcggccac gccggtgccg ttgctgctaa gaccgagcat ctggttgttc acgagcgcgt
     2401 cgctcagaaa ctgcgtgctc tcgtcgttgg ccagggcgtt ctgatcgaag tagttggtca
     2461 ggtcgagcct gccggctgtc agcgccagcc gctgtccgaa gagctcggtc cggagccacg
     2521 cctcgcgaag gttcagttgg ttctgcgcga ccaggcgcgc cgtgtatccg ttcaacagcg
     2581 acagtgtagg aatctcggtg tcgggcggcg acccgctgag ccctacgatg tcggcgaaga
     2641 agaccgtgta ctgggcgatc cctgccgtga agaagaggtc cgccgacgcc agggcatacg
     2701 cgccgctggt ggggtccatc gtcccctgag tgaggttggc aaactgcgtt gacacggcgg
     2761 cgtcaacgcc gaccgagacg ctgtgctcgg cggcttcctc gagcgcctcc tcgatcttct
     2821 cgttgatcgt ctggcggcgg tagacgcgct cacgctcgta cgtcacgaca ggcgtcgagc
     2881 ccggcacctg ggtgtcggtt tcgttgccgc tcgcgtccac gaacttttca acacgccgga
     2941 cgcgtgtttc cggatccgac agcacggtgt tcgactcgag atcggtaatg cgctgctcca
     3001 gcgtcgcgat ccgctcgagg agctgctggc gctcgaactc ggctgccgcc gccgcggatg
     3061 ccgcattcag ggtgatgtcg agcggcgcag tccggtccat ctccaccgcg acgccggccc
     3121 gtctgaacgc cgtctggccg gccgaggtga tggtgatgtc atatctcccg accggcagcg
     3181 cgccgaacga gtacgcaccc tgggcatcgc tcgtcgcggt gaacttggcg ccgttctcgg
     3241 cgttcacggc cgtcacggcc gcttcggcaa ccggcgcacc ggcatcgttc cggacgacgc
     3301 ctcgaatgcc gccgcggtgg tcggccatgt tcatcggcgc ctgggcagcc ggcgtctgcg
     3361 ctgcggcgga tccgcaccag aacacgagca tggcggcgcc agtgcctcgc gccagccgct
     3421 ttctccagcc a
//
LOCUS       DBNGSH010000450         8613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_721787, whole genome shotgun sequence.
ACCESSION   DBNGSH010000450 DBNGSH010000000
VERSION     DBNGSH010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8613
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_721787"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="AC16CY_10375"
     CDS             complement(<1..548)
                     /locus_tag="AC16CY_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3354237.1"
                     /translation="MRSLLLVFALLAQAPALPSEIPLFPLPETTLFPGVSRPFVIFEP
                     RYRDMIGDALKGDKIIGMVRLRPGFEKDYEGRPPIYGIGCAGTIEKYELLPDGRYLIL
                     LRGLATFRVLSEDQRKPYRLARVEAVPELLGDEDRVTLGRLRNQIEDLLFKILPLDAE
                     PPDPSLDDAELVNIVAQNLEMP"
     gene            complement(567..1322)
                     /locus_tag="AC16CY_10380"
     CDS             complement(567..1322)
                     /locus_tag="AC16CY_10380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3354238.1"
                     /translation="MKKSRTTPDARGWEELAQLDPLWAVASSPQLRHGKWNEEEFYAV
                     GRRKVRKLMKRLDALGVPARTARALDFGCGAGRLTLPLADHFAMVVGVDIAPSMLERA
                     RTRAAGRPGLEFRLDETGDQSLLAGESFDLVYSGLVLQHLPSIGHALACLERLCATVG
                     PGGALVVQVPVWLREQGLRRIRQRLYILLRRLGVPARVLYTGAGLHPMTMICVPRERV
                     ESCLQAAGLAIVHADERTDDDIVSLTIYAARRG"
     gene            complement(1557..3113)
                     /locus_tag="AC16CY_10385"
     CDS             complement(1557..3113)
                     /locus_tag="AC16CY_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acido-empty-quinoprotein group A"
                     /protein_id="HIR3354239.1"
                     /translation="MRKLLPHPLVTTLFIALAVSRVTSQGLDPAALLKPTSDSWPTYH
                     GDYTGQRHSRLTQITPANVHQMTLAWAFQTGQTQQIKATPILVNGVVYVTTPDNVWAI
                     DARSGRQIWRYAYPANDGFHIGHRGVAVYKDFVYLTTPDARLVALDARDGKVRWNIAI
                     ADSKRGYWSTNAPLVIRNHLIVGVSGDFDNLPGILRSFDADTGKPQWTFYSTPPPGTP
                     DSISGGATGGQMWMTGTYDPGLNLLYVGTGNPTPVLNGAMRPGDNRWTGSIVALNPDT
                     GALVWGFQVSPHDTHDWDAAEVPVLVDRDFGGTPRKLLLQASRNGYYFVLDRTNGKNL
                     LTAPFATVNWAKEIDKEGRPIPNPDKEPARDGRLVAPDEAGGTNYRSPSFDPATGLLV
                     VSAHDGYGLYFFKPEHGEYGWAGASPAVHGRGVLRAIEYQTGRVRWSHDLHGGAGSAG
                     VLTTASGLTFTGDSAGSALALRTSDGATLWHSGIGRVGNSPITYELDGRQYVIVGGGS
                     ALYAFALAAP"
     gene            complement(3145..4044)
                     /locus_tag="AC16CY_10390"
     CDS             complement(3145..4044)
                     /locus_tag="AC16CY_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3354240.1"
                     /translation="MQSRHLHTSAPSRLRRCAASAWQAHLRTACALWLLSLLVLVPLE
                     AQRGQASPAAQRPPQTTTPQQYPREQVDAGRVRFAAQCGFCHGRDAAGGEGGTDLTRS
                     ALVAEDVRGNRIIPLVRSGRTDKGMPAFTLPDAELAAIVAFIHDRKSQADSVAGGRRV
                     VAASDLQSGNVEAGRRYFEGACVKCHSANGDLAGIASRYQGLALLQRMLNPGAGRGGG
                     SARAVPKVTVKTASGETVSGTLTYRDEFTIAITDASGWHRSWPTRLVTFTVDDPLQAH
                     VEQLGKYTDADMHNVLAYLQTLK"
     gene            4148..5014
                     /locus_tag="AC16CY_10395"
     CDS             4148..5014
                     /locus_tag="AC16CY_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3354241.1"
                     /translation="MSRFSTTILPLLLTLCVMWRVELGSAHAAAPQAASAPAFDAASI
                     RRREPGDRPPATRMLVTPGRISFQAVLLRDCLQWAFGVAEWQIADGPAWIGRGVRWDI
                     EARAEGAATDAELRQMFRALLAERFGLRASRDARETRVYLLTVANAGPRLRRAADNAP
                     KSDDWRRSPLFQQSKAGPGSVTIRELTTERVSMRYFTEYLSRQLGAPVLDRTGLEGDF
                     AFALEWQADAPPRDAQGPAGVPPAPRQPGNEMFGSAGIEALRDQLGLALAFTRAPVDM
                     LVIEAVEEAREN"
     gene            complement(5122..5619)
                     /locus_tag="AC16CY_10400"
     CDS             complement(5122..5619)
                     /locus_tag="AC16CY_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HIR3354242.1"
                     /translation="MPDVITSSILPLRGQKVLLDSTLASLYRVRTKALNQAVRRNRAR
                     FPADFMFRLTKPELADLRSQFVTSSSRAHGGRRTRPYAFTEQGVAMLSSVLKSSRAIA
                     VNVEIMRAFVRLRSMLAAHAELAARIDELEQKYDGQFRAVFQTIRQLIEPAPLPRRRI
                     GFRKT"
     gene            5912..6949
                     /locus_tag="AC16CY_10405"
     CDS             5912..6949
                     /locus_tag="AC16CY_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354243.1"
                     /translation="MKPLITRYRATAGAGLVAIAIAIALTGVTERAVAQQPAADSLDV
                     VQLRPNFHVIAGAGGNIVVQLGPDGAILVDSGSTDKVEVVLAAIRKLTPLPIRYIINT
                     SMDADHVGGNDRLAKAGLSILPGAVAAGAGLNDDLVSNSGRASVLAHENVLTRMTAAT
                     GPQALPFAHFPTKTFAFRQYSMYLNGEGIQVIHMPAAHTDGDTIVFFRRGDVIATGDI
                     IDTTRWPFIDVKRGGSVQGELAALNRLMDMSILNVPLQWHPDRTLLVPGHGFVYDKFE
                     LLEYRDAVTVVRDRVQNLIDEGKTLAQVKAADPTLGYKSQYGADKGAWTTDMFVETVY
                     NELAANKGKKE"
     gene            6946..7746
                     /locus_tag="AC16CY_10410"
     CDS             6946..7746
                     /locus_tag="AC16CY_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354244.1"
                     /translation="MSRVLVVVASAIIVCGLARIEAQRGGAPAPPRAPRAGAPVDLTG
                     YWVSIVTQDWRWRMVTPAKGDYGGVQMTPAARKIADAWDPARDEAAGEQCKSYGAPAL
                     MSVPGRLHITWQDDTTLKVDADAGTQTRLLRFGKIDTPADAPRTWQGISQAQWLTPRP
                     NVPLQLRPADRAADTPPLRPSGGSLQVVTRHLRSGYLRKNGVPYSENAVLTEYWDYYK
                     RPNGEEWLTITTQVEDPEYLPNVRLTAPIFKKEPNGSKWDPTPCSARW"
     gene            7797..>8613
                     /locus_tag="AC16CY_10415"
     CDS             7797..>8613
                     /locus_tag="AC16CY_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354245.1"
                     /translation="MPHSNMRYVGFSALVAAALTAAPASAQIDLTGTWQRVAANDNGA
                     SREPVDLLGMPLSADGRAKALSYDIAALSATERQCQMYTPYYALVGPFPLQFVMVPDP
                     ITQKLLAWKILGWGDRDETVIWMDGRPHPSKYAPHPHGGFTTGVWEGDTLTAVTTHFK
                     LGDIKRHRGFNSDRATLTYRFMRHGDTLTVTGILEDPVYLAEPYPLTEVYRLTTNPNH
                     FPLTACEPIEELPRLHEDPTLAPAYLPGKHPALNEVTEKHNIPLDAVLGGPETM"
BASE COUNT         1456 a         2815 c         2931 g         1411 t
ORIGIN      
        1 tccggcatct ccaggttctg cgcaacgatg ttgacgagct cggcgtcgtc gagcgaggga
       61 tccgggggct cggcgtcgag cggcagtatc ttgaagagca aatcctcgat ctggttgcgc
      121 agcctgccga gcgtgacgcg gtcctcgtca ccgagcagct cgggcaccgc ctcgacgcgt
      181 gccaggcgat agggcttgcg ctgatcctcg ctgaggacgc ggaaggtggc gaggccgcgg
      241 agcaggatga ggtaccggcc gtcgggcaac agctcgtact tctcgatcgt gccggcgcag
      301 ccgatgccgt agatcggcgg gcggccctcg tagtccttct cgaatcccgg acgcagtctc
      361 accatgccga tgatcttgtc gcctttcagg gcgtcgccga tcatgtcgcg gtaccgcggc
      421 tcgaagatca cgaacggcct cgacacgccc gggaagagcg ttgtctcagg cagcggaaac
      481 agcgggatct cggaaggaag cgccggtgcc tgggcgagca gcgcgaagac caacagtaat
      541 gagcgcacgg ccacagtcta gaacgttcat cccctccgtg ccgcgtagat cgtcaggctg
      601 acgatgtcgt cgtctgtgcg ctcgtcggca tgcacgatcg cgagcccggc agcctgcagg
      661 cacgactcga cgcgttctcg cggcacgcag atcatcgtca tcgggtgaag gcccgcgccg
      721 gtgtacagca cgcgtgccgg aacgcccagg cgccgcagca ggatgtagag acgctgccgg
      781 attcgccgca gcccctgttc ccggagccag actggaacct ggacgaccag cgcaccgccc
      841 gggccgaccg tggcacaaag ccgttccagg caggcaagcg cgtggccgat cgatggcagg
      901 tgctgcagca cgagcccgct gtagacgagg tcgaaggact cgccggccag cagcgattgg
      961 tcgcctgtct cgtcgagcct gaactcgagt cccggccgcc ccgccgcgcg cgtgcgcgcc
     1021 cgctcgagca tcgacggggc gatgtccacg ccgacgacca tggcgaagtg gtcggcgagg
     1081 ggcaacgtca ggcggccggc gccgcatccg aagtcgagcg cgcgcgccgt gcgggcgggg
     1141 acgccgagcg cgtccagacg cttcatcagc ttgcgcacct tccggcgccc gacggcgtag
     1201 aactcctcct cgttccactt gccatgacgc agctggggag aggaggcgac tgcccacagg
     1261 ggatcgagct gggcgagctc ttcccaccca cgcgcatccg gcgtcgtgcg cgatttcttc
     1321 aagtgatggg aagcctagtc ccagaggtca cttgaatcga ccatgagatc cgtcgttcac
     1381 ccttctctgt tctcgcttct ccgttcgtgt tcacgttcgg gggtgatacc gctgacgtcg
     1441 cgaagaaccc cagagggaac gccgaactcc ccaaccgaag gtgaacacga acagagaagg
     1501 tcagaaccga gcagcgtgaa cacacgatct cttgctcaac tcgagtgatt cgacgttcag
     1561 ggcgccgcga gcgcgaatgc gtatagcgcg ctcccgccgc cgacgatcac gtactgccgc
     1621 ccgtcgagct cgtaggtgat cggcgagttg ccgacgcggc cgatgccgga atgccacagc
     1681 gtcgcgccgt cgctcgtgcg cagcgcgagc gcgctgccgg ccgaatcgcc cgtgaacgtc
     1741 aggcccgatg ccgtcgtcag cacgcctgcg gagccggcgc cgccatggag gtcgtgactc
     1801 cagcgcacgc gtcccgtctg atactcgatt gccctgagca cgccgcggcc gtgcacggcc
     1861 gggctcgctc ccgcccagcc gtactcgccg tgctcgggct tgaagaaata gaggccgtac
     1921 ccgtcgtgcg cgctgaccac cagcagaccg gtcgcgggat cgaagcttgg cgatcggtag
     1981 ttggtcccgc cggcttcgtc aggcgcgacg aggcgcccgt cgcgggccgg ctccttgtcg
     2041 gggttgggga tcggacggcc ttccttgtcg atctcctttg cccagttgac ggtcgcgaac
     2101 ggtgccgtca gcaggttctt cccgttcgtg cgatcgagca cgaagtagta gccgttgcgc
     2161 gacgcctgca gcagcagctt gcgcggcgtg ccgccgaagt cgcggtccac gagaacgggc
     2221 acttccgcgg cgtcccagtc gtgcgtgtcg tgcggcgaca cctgaaatcc ccagacgagc
     2281 gcgccggtgt ccgggttgag cgccacgatg ctgcccgtcc agcggttgtc gccgggacgc
     2341 atggcaccgt tgaggacggg agtcggattt cccgttccaa cgtagagcag gttcagcccg
     2401 ggatcgtagg tgccggtcat ccacatctgg ccgcccgtcg cgccgccgct gatcgaatcc
     2461 ggcgttcccg gcggaggcgt gctgtagaag gtccattgcg gcttgcccgt gtcggcatcg
     2521 aaggaccgca ggatcccggg aaggttgtcg aagtcgcccg agacgccgac gatcaaatgg
     2581 ttccggatga cgagcggcgc gttggtcgac cagtagccgc gcttgctgtc ggcaatcgcg
     2641 atgttccacc tgaccttgcc gtccctggcg tcgagcgcga ccagacgggc gtctggcgtg
     2701 gtgaggtaca cgaagtcctt gtagaccgcg acgccgcgat gcccgatgtg aaagccgtcg
     2761 ttggcggggt aggcgtagcg ccagatctgc cggcccgatc gggcgtcgat cgcccacacg
     2821 ttgtcgggcg tcgtgacgta cacgacgccg ttcaccagga tcggcgtggc cttgatctgc
     2881 tgcgtctgtc ccgtctggaa cgcccaggcc agcgtcatct ggtgcacgtt ggccggcgtg
     2941 atctgcgtca ggcggctgtg ccgctgcccc gtgtagtcgc cgtgatacgt cggccaactg
     3001 tcggaggtcg gcttcaacag ggcggctgga tcgaggccct ggctggtgac gcgcgacacc
     3061 gcgagcgcga tgaagagggt cgttaccaga ggatgaggga gcagttttcg catcgttgtc
     3121 ctcgggtccg gctcaagccg gacactactt cagcgtctgc agatacgcga gcacgttgtg
     3181 catgtccgcg tcggtgtact tgccaagctg ctcgacgtga gcctgcaacg gatcgtcaac
     3241 ggtaaacgtg accaaccgcg tgggccagga tcggtgccag cccgacgcat cggtgatcgc
     3301 gatcgtgaac tcgtcgcggt acgtcagggt gcccgagacg gtctctcctg aagcggtctt
     3361 cacggtgacc ttcggcacgg cgcgagccga tccgcctccc cggcccgcgc ccgggttgag
     3421 catgcgctgc aggagcgcga gcccctggta tcgactcgcg attccggcga ggtccccgtt
     3481 cgcggaatgg cacttcacgc acgcgccctc gaagtaccgg cggcccgctt cgacattccc
     3541 gctctgcagg tccgacgcgg cgacgacgcg gcggccgcct gccaccgagt ccgcctgcga
     3601 cttccggtcg tggatgaagg cgacgatggc cgcgagctcg gcgtcgggga gtgtgaacgc
     3661 gggcatgccc ttgtcggtcc gcccggaccg gaccagcggg atgattctgt tcccgcgcac
     3721 atcttcagcc accagcgcgg acctcgtcag gtccgttccg ccttcgccgc cggccgcgtc
     3781 gcgtccgtga cagaagccgc actgcgcggc gaaacgcaca cggccggcgt cgacctgctc
     3841 ccgcgggtac tgttgcggcg tcgtcgtctg cggcgggcgc tgcgccgccg ggctggcctg
     3901 gccgcgctgt gcctcgagcg gcacgaggac gaggaggctg aggagccaga gggcgcaggc
     3961 ggtacgaagg tgcgcttgcc acgccgaagc cgcgcagcgg cgaaggcggg agggtgcgga
     4021 ggtgtgaagg tgccgtgact gcatgccgcg atgagtattg tcgtcctgcc caggcgcgtc
     4081 aattgcccgg atgacggcaa gagcgctgga gcacttacga tacgattgaa aacgggcagc
     4141 atccagcgtg tccagattct cgacgacgat cctaccgctg ctgttgacgt tgtgcgtgat
     4201 gtggcgcgtc gagcttggct ccgctcatgc ggccgcgcct caggccgcat ccgcgccggc
     4261 cttcgacgcc gcgtccatca gacgcagaga gccgggcgat cggccgcccg cgacgcgcat
     4321 gctcgtcaca ccagggcgaa tcagctttca ggccgtgctc ctcagagact gcctccagtg
     4381 ggcctttggt gtcgcggaat ggcagatcgc cgacggaccg gcgtggatcg gccggggagt
     4441 gcgatgggac atcgaggcca gggccgaagg ggctgcgacg gatgccgagc tccggcagat
     4501 gtttcgcgcc ctgctggccg agcggttcgg gcttcgcgcg agccgcgacg cgcgggagac
     4561 gcgcgtctac ctgctcaccg tcgccaacgc cgggcccagg cttcggcgcg ccgctgacaa
     4621 cgcaccgaag agcgacgatt ggcgcaggtc tccgctcttt caacagtcga aggccggccc
     4681 aggctcggtg accattcggg aactgacgac cgagcgcgtg tcgatgcggt acttcaccga
     4741 gtatctgtcg cggcagctcg gcgcgccggt tctcgatcga accggactgg aaggcgactt
     4801 cgcgttcgcg ctcgagtggc aggcggacgc gccgccgcgc gacgcgcaag ggcctgcggg
     4861 agttcctccg gcgccccggc agcctggcaa cgagatgttc gggtccgccg gcatcgaggc
     4921 gcttcgcgat cagctcggcc tcgcgctggc attcacgcgc gccccggttg acatgctcgt
     4981 catcgaagcc gtcgaagaag ccagggagaa ctgaacacgt cctggctgat gggccgaaaa
     5041 cttgagatca cagaacgtga tctcgagtgc ggaagcactg gcgccaatga ccgtgcgcgt
     5101 cagacggcgt gaagatgggg cctacgtctt ccgaaagccg attcgtcggc gcgggagggg
     5161 cgcgggttcg atgagctgcc ggatcgtctg gaagacggct cggaattgac catcgtattt
     5221 ctgctcgagt tcgtcgatcc gcgccgccag ctcggcgtgg gccgcgagca tcgatcgcag
     5281 ccgtacgaag gcacgcatga tctccacgtt caccgcaatg gctcgcgagc tcttgagcac
     5341 gctcgacagc atggccacgc cctgctcggt aaacgcgtac ggtcgcgtgc gtcggccgcc
     5401 gtgggcacgg gaacttgagg tcacaaattg tgacctcaag tcggcgagtt cgggcttggt
     5461 gagccggaac atgaagtcgg cgggaaagcg cgcgcgattt cgtcgaacgg cctggttcaa
     5521 cgccttggtc ctgactcgat acaacgacgc cagcgtcgag tccagcagca ccttctggcc
     5581 gcgcaggggc agaatcgaac tcgtgatgac gtcgggcatc ggggtcttcg ccggagcggt
     5641 cttgttcact gcgcccgagc ctggtgcaag cgggatgcct tgcgcgcctg cgtccgaatc
     5701 ggtcgatcgt catcgcgatc caacggcccc gcggcttcga gacgtgcaag gaatgcgcgt
     5761 tcaccacgct gttcacgtag cacggattgc gtacccggtg ccccggagag gtggccggtc
     5821 ccctaccctc gtgtcagcgc gcggccttga cttgtcgtat ctctcctgag cagaatccga
     5881 aatccacaag acggggcggg gagcccacga cgtgaagcca ctcatcacac gttacagagc
     5941 gactgcagga gcggggctcg ttgcgattgc catcgcgatc gcgctcaccg gagtgaccga
     6001 gcgagcggtt gcccaacaac cagcggccga ctctctcgac gtcgtccagc tgcggccgaa
     6061 cttccacgtc atcgccggag cgggcggcaa catcgtcgtg cagctcggcc ccgatggcgc
     6121 catcctcgtc gattcgggat cgacggacaa agtggaggtg gtgctggcgg cgatccggaa
     6181 gctgacgcct cttccgatcc gctacatcat caacacgagc atggacgccg atcacgtcgg
     6241 cgggaacgac aggctcgcca aggccggact cagtattctc ccgggcgctg tcgcggcggg
     6301 ggcggggttg aacgacgacc tggtctccaa ctcgggcagg gccagcgtgc tggcgcacga
     6361 gaacgtgctg acgaggatga cagcggcgac gggtccgcag gccctgccgt tcgcacactt
     6421 tccgacgaag accttcgcct tcaggcagta ctccatgtac ctgaacggcg aaggcattca
     6481 ggtcattcac atgccggcgg cgcataccga cggggacacg atcgtcttct tccgacgtgg
     6541 cgacgtgatt gcgaccggcg acatcatcga cacgacccgc tggcccttca tcgacgtcaa
     6601 gcgcggcggg agcgtccagg gcgagctcgc cgcgctgaac cgtctgatgg acatgagcat
     6661 cctcaacgtg ccgctgcagt ggcaccccga ccgcacgctc ctggtgcccg ggcacggttt
     6721 tgtgtacgac aagttcgagc tgctggagta ccgcgacgcg gtgacggtcg tccgcgacag
     6781 agtgcagaac ctgatcgacg aagggaagac gctcgcgcag gtgaaggccg ccgatcccac
     6841 actcggatac aagagccagt acggggcgga caagggtgca tggaccacgg atatgttcgt
     6901 cgagaccgtc tacaacgagc tggccgcgaa caaggggaag aaggaatgag ccgagtgctg
     6961 gtggtggtgg cgtcggcgat catcgtgtgc ggcctcgcgc gcatcgaggc ccagcgcggg
     7021 ggcgccccgg cgccgccacg cgcgccgcga gcgggggcgc ccgtcgatct cacgggttat
     7081 tgggtgtcga tcgtcacgca ggactggcgc tggcgcatgg tgacgccggc caagggcgac
     7141 tacggcggcg tgcagatgac tcccgccgcc agaaagattg ccgatgcctg ggatccggcc
     7201 cgggatgaag ccgccggcga gcagtgcaag tcctacggcg cgcccgcgct gatgagcgtc
     7261 ccggggcggc ttcacatcac gtggcaggac gacaccacgc tcaaggtcga tgccgatgcg
     7321 ggaacgcaga cgcgactcct gcgctttggc aagatcgaca cgcccgcgga tgcgccgcgc
     7381 acatggcagg gcatctccca ggcgcagtgg ctgacgccgc gcccgaacgt gccgctgcag
     7441 ttgaggccgg ccgaccgtgc cgcggacacg cctccgcttc gcccgtctgg cggctctttg
     7501 caggtcgtca ccaggcacct tcgctcggga tacctgcgga agaacggcgt tccctacagc
     7561 gagaacgccg tgctgaccga gtactgggat tactacaagc ggccgaacgg cgaggagtgg
     7621 ctgacgatca cgacgcaggt cgaagatccc gaatatctgc cgaatgtgag actgacggcg
     7681 ccgatcttca agaaggagcc taacgggagc aagtgggatc ccacgccctg ttcggcgagg
     7741 tggtagttac ggattcgtaa gggggtcggg agtcattttt cgaggacggt catctcatgc
     7801 ctcatagcaa catgcgctac gttggattct ccgcactcgt tgcggcagcg ctcacggcgg
     7861 ctcctgcgtc cgctcagatc gatctcaccg gtacctggca gcgcgtggct gcaaacgaca
     7921 acggcgcctc gcgggaaccg gtcgatctgc tggggatgcc gctcagcgcc gatggccgcg
     7981 cgaaggcgtt gagctatgac atcgccgccc tctcggcgac cgagcgccag tgccagatgt
     8041 acacgccgta ctacgcgctc gtgggcccgt tcccgctcca gttcgtcatg gtgccggacc
     8101 cgatcacgca gaagctgctg gcctggaaga tcctcgggtg gggcgaccgt gacgagacgg
     8161 tgatctggat ggacggacgg ccgcatccgt cgaagtacgc gccgcatccg cacggcggct
     8221 tcaccaccgg cgtgtgggag ggcgacacgc tgaccgccgt gacgacgcac ttcaagctcg
     8281 gtgacatcaa gcgccatcgc ggtttcaaca gcgatcgagc gacgctgacc tatcgcttca
     8341 tgcggcacgg cgacacgctg accgtcaccg gcatcctcga ggaccccgtg tatctggcgg
     8401 agccgtatcc gctgacggag gtctaccgcc tcacgacgaa cccgaaccac ttcccgctga
     8461 cggcatgtga gccgatcgag gagctgccgc gcctgcacga ggacccgacg ctcgcgcctg
     8521 cctatctgcc cggcaagcat ccggcgctga acgaggtcac cgagaagcac aacattccgc
     8581 tcgacgccgt gctcggcggt cccgagacga tgt
//
LOCUS       DBNGSH010000451        19742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2249755, whole genome shotgun sequence.
ACCESSION   DBNGSH010000451 DBNGSH010000000
VERSION     DBNGSH010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 19742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19742
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2249755"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="AC16CY_10420"
     CDS             complement(<1..385)
                     /locus_tag="AC16CY_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIR3354246.1"
                     /translation="MTTPVSYTNASVKHPRIAGVKTKPLRLIPDERGWLMEILRADEA
                     ELFTRFGQVYVSATYPGVVKAWHYHKVQVDNFACVAGMVKLVLVDTRPDSPTNGAINE
                     FFLGTQNPTLVQVPNLVYHGWKCISP"
     gene            complement(393..1460)
                     /locus_tag="AC16CY_10425"
     CDS             complement(393..1460)
                     /locus_tag="AC16CY_10425"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HIR3354247.1"
                     /translation="MKGLILSGGKGTRLRPLTYTSAKQLVPIANKPVLFYGIEAIADA
                     GIKDIGIVVGDTHAEIRAAVGDGSRWGVRVTYIAQDAPLGLAHAVKISRDFIGADPFV
                     MYLGDNLLDKGITHFVEEFRRERPAAQILLTKVPDPQMFGVAELRNGHVVRLVEKPKE
                     WIGDLALVGVYMFGPQVFDAVNRIKPSFRNELEITDAIQDLIDHGLTVRPHIVEGWWK
                     DTGKLEDMLEANRLILDTFETRVEGSVDAESRIEGKVVIEAGAVVERSTIRGPAIIGQ
                     GARIVQAYVGPFTSIMSGVEIQETEIEHSIVLEGSSLRNLANRVTDSLIGKNVRIYRE
                     PVKPSAYRFMLGDNSEVGIRW"
     gene            complement(1467..3764)
                     /locus_tag="AC16CY_10430"
     CDS             complement(1467..3764)
                     /locus_tag="AC16CY_10430"
                     /inference="COORDINATES: protein motif:HMM:NF040057.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR3354248.1"
                     /translation="MARVILQSCVVCLLGAAPALAQQGGQPDPAAETQGKPGGSLIDR
                     LRSFGGPMEIVSATHVRWTGGVEQEWDGQTKFFAEQIDLYLDTNRLVATGNVVFTNRT
                     GHIAAESAEFDLDRNTAVFHVASGLMPMGAAGAIRQAGSPDTEVYFWGKTIEKVGDTE
                     YRISGGGFTTCVQPTPRWDMATDDFVLKVDDYAVARNTVFRVKGVPLLYLPVVYYPIQ
                     DDRATGFLLPTYSASRVQGNTISNAFFWAINRSQDATFFYDWFSRSGYGLGTEYRYVA
                     APESSGNLRLQRLILNEVRVTDGGITTTLPASQSFVLRGNMNHAITPRIRARARFDYF
                     SDIVSQQLYQQNFYMASQNSRYIEAGLTAAYGPVSSGVLYQRNELFTSQTDSYVYGST
                     PRLTSSVAPQRLFGSPVYASVNAEYAFLPDRTVTDGVVVRDEGYGRVDVTPTVRVPLS
                     KLTYLSVNTSASYHTTYYTRSVDSTGNTVEDPYHRRFMSVRSDIVGPVLTRIWDRPDS
                     VFAERLKHVIEPAFSVDVTSQIADYASTPVVGDVSDFVVGGSTRFTFGVTNRLFARGK
                     PREQARGQTREFLTVGLQQTYYSNPESARYDSTYQSTYGYGRPQDLSPVALTARVSPS
                     VALDGNLRLEYDVAGLGLQTLSAGGNVNLRTLSVNANYSHRRSLPTYSGSDYLSAATT
                     SRWLDGRATGTYSLSWDIAQSKIVSQTVAGSYVAQCCGLQLELQKFDYPEGFGYPSDV
                     RFNVGFTLAGLGTFSNFFGALGGQR"
     gene            complement(3777..5081)
                     /locus_tag="AC16CY_10435"
     CDS             complement(3777..5081)
                     /locus_tag="AC16CY_10435"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3354249.1"
                     /translation="MFERPVFERLLALETYGIKLGLDNISRLCEALNHPERAFRSLHV
                     AGTNGKGSVTAMAHAVLRAAGVRAARYTSPHLSNLAERFVIGDEPVHTALLESAVDDV
                     LRCADRLRAEGILSVQPTFFEATTAAAFELFRRGGVEVGVVEVGLGGRFDATNVIAPL
                     AGAITTIHFDHQQHLGDTLGAIAFEKAGIIKPRMKVVMGNLAGEAAAVIRRCAEEREA
                     TLVEAMAGVRAETEMVDGRARLTLTTPAGSYGPVMLGLRGEHQVGNAIVAVRLLEAAA
                     ESSVTIPRTAVEQGLAAAEWPARLELLSLEGGRRVLLDAAHNPEGARALADYLRRWHP
                     ERPPLVIGVMKDKNVADIARALLPVVSSVVATAAPTPRAIPAPDLARQLRAAGASDVR
                     TEPDPNVAIETALERTDVVCVAGSIFLAGAVRDRLRQRAILR"
     gene            complement(5088..5996)
                     /gene="accD"
                     /locus_tag="AC16CY_10440"
     CDS             complement(5088..5996)
                     /gene="accD"
                     /locus_tag="AC16CY_10440"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR3354250.1"
                     /translation="MAWFKKTRKPIASSTAENPSRVPEGLWVKCPDCSQLIFKKDLDS
                     NLSVCPKCSHHFRLRAAERLAALLDEGQYEEHFPDLTSTDPLRFTDTKPYRERLQKTM
                     EATGLKDSVIVATGQLDGLDVVVAAMEYTFIGGSLGVVAGEKITRAIEMALARRIPAI
                     VVSCSGGARMMEGALSLMQMAKVSAALSRLDRAGLPFISVLTDPTTGGVTASFAMLGD
                     LNIAEPKALIGFAGPRVIEQTIRQKLPEGFQRSEFLLEHGMLDLVVDRRELKATLARA
                     LRFMRATASAAPEPAPALAGAAGETT"
     gene            complement(6032..6670)
                     /locus_tag="AC16CY_10445"
     CDS             complement(6032..6670)
                     /locus_tag="AC16CY_10445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR3354251.1"
                     /translation="MSFQYIAIEGPIGVGKTSLADRLAVRLDAATLFEERENPFLDDF
                     YAGRPGAALQAQLFYLLNRHRQQLSMRQGDLFAQTTVSDYLFDRDKIFAYLNLDDNEL
                     FIYQRLYDLLAADVPSPDLVIYLQAPTDVLVRRLKDRAAKTDGETLDPSVEYLRELNE
                     AYQHFFFHYSATPLLVVETSQFDLAGSDEALDDLIRQINAMGRGTQYYVPRA"
     gene            6819..8885
                     /locus_tag="AC16CY_10450"
     CDS             6819..8885
                     /locus_tag="AC16CY_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3354252.1"
                     /translation="MPPAPVVPVTTWEEKIGWILRLEDERLIRDPNPPPPRVIQAATS
                     RLPEILAPPPPSDLILLLGDQQARVRRRAALALGRVGLAEAVEPLAGLLTDEEFEVRQ
                     MAAFALGLVGESAARPALLAALNNDPELVVQGRAAEALGAIGDRADADAIAAMVQRQV
                     SAGVLGGLDPDDIGYPKAGGVEAVRLGVYALARLRAYDALAGAILDAQGKPVSSWWPI
                     AYALQRCGDDRAAPALMALLPTPGRFTASFAIRGLAAFKAVHAADALREIVTERTRDP
                     AIVIQSVRALSQFGDRASVPVLTRMLADRSVTGALELEVMSALGNLADESDTDVLLEL
                     VSDSKPSVRATAFRALARVEPATFLAALAGLDADPDWTVRVAVANALGGLPAEQSIPK
                     LNQMLQDRDGRVIPAVLTALGATKSPGVDRLLLDRLRSDDFMVRASAANALAEMKAAG
                     AAGPLAQAYRAADGDATYVARAAVLSALSRLDPAAARPVLEDALKDRDWAVRVRAVAL
                     LREQGATGLDQAIRPAPAGRVVQEAERQALATPQFSPHAFIETDKGTVEIELAVLDAP
                     ETVGNFMSLARKGFFNAVAIHRVVPDFVVQDGDPRGDGEGGPGYSIRDEINQRPYLLG
                     TVGMALDWEDTGGSQFFITLSPQPHLDGRYTVFGHVVNGMDVADRLLPGDVVRRVRIW
                     DGVKEQ"
     gene            complement(9509..10588)
                     /locus_tag="AC16CY_10455"
     CDS             complement(9509..10588)
                     /locus_tag="AC16CY_10455"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3354253.1"
                     /translation="MKIRVGVIYGGRSGEHEVSVVSAASIFRHLDRSRYDLVPIRIAR
                     DGRWLLGTREPSALTAAEVEDENPAGGALQVAEPTRAVSGAVDVVFPVLHGPYGEDGT
                     VQGLLELANVPYVGAGVLGSALGMDKAMAKAVFAAHGLPLAPHVTILAHEWEADSGKI
                     SAEIARKLRFPVFVKPANLGSSVGISKADADTELGAAMSLALEFDRKVVVEAAVPHAR
                     EIECGVLGNDDPQASLPGEVVVTHRDGFYSYEAKYLDPDGAATKIPADLSPELTDRVR
                     RLSVEAFKALDLCGMARVDFLLDDRGGGLYLNEVNTIPGFTAISMFPKMWEATGLAYP
                     DLLDKLVTLALERHERKQRLRTSRT"
     gene            10672..11838
                     /gene="tgt"
                     /locus_tag="AC16CY_10460"
     CDS             10672..11838
                     /gene="tgt"
                     /locus_tag="AC16CY_10460"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR3354254.1"
                     /translation="MTPDPFRFELLRTDGAARLGEVHTPHGVVHTPAFMPVGTQGSIK
                     GALFRDVEDAGAEIVLGNTYHLYLRPGDERICRLGGLHRFIGWNRPILTDSGGFQVFS
                     LSERRRITEDGAEFRSHLDGSLHHLSPERSVDIQARLGSDVAMVLDECLAYPSTHEQT
                     AESVGRSLRWAERCRRRLEALRSSPPPDVAVMNPGQAQFGIVQGGVFADLRRESAEAT
                     IAIGFDGYAIGGLSVGEPVDVMYDMVEGTARHLPEREPRYLMGAGTPEDLVECVGRGI
                     DLFDCVLPTRNARNGQLFTSQGRINIKNARYADDDAPLDANCRCYTCRRHSRAYLRHL
                     YLAGEMTAGALNTLHNLTFYLDTMKRIREAIVFGRFDMFRQEFLQSVARLSYES"
     gene            11835..12173
                     /gene="yajC"
                     /locus_tag="AC16CY_10465"
     CDS             11835..12173
                     /gene="yajC"
                     /locus_tag="AC16CY_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR3354255.1"
                     /translation="MNLLDPFLVAVAPQGSPSPWLQIVPFILILGIFYFVMVLPMRKR
                     QQKVQAFLAALKVGDKVITSGGIFGSITRVSDQAVQLQIANNVRIDVSRQAIVGYQGQ
                     EPVVPEGNQQ"
     gene            12170..13723
                     /gene="secD"
                     /locus_tag="AC16CY_10470"
     CDS             12170..13723
                     /gene="secD"
                     /locus_tag="AC16CY_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR3354256.1"
                     /translation="MIKNLQWRLGIIAVVIGLSVWAFVPPREKVKLGLDLKGGVHLVL
                     RVKTDDALRVETETTVERLRDSLAREGVQVSKVEVTSPTEFRVEGIQDDAGFRAQAAA
                     DAESVFDRSPEAGGYTYRIKPNVANQLRDQTVAQALETIERRVNELGVAEPIVARYTE
                     QDQIVVQLPGVSDVRRAKDIIKSTARLELRLMDQGPFPSREAALQAVNNALPPDAEIL
                     PGRSEGTGTAGTAGTSYYLVKRVAAVAGNDLRNAQQSLDEYNRPAVAFTLKQDAAERF
                     GKFTQSNLGRPMAIVLDERVMSYATIQGRITDNGQITGITRDEMLDTVITLKSGALPA
                     SMDYLEERTVGASLGEDSIRSGVTAAVGGLLLVMVFMLFYYKLAGLNALTSIIVNLII
                     LLGLMASIDASMTLPGFAGFILTIGIGVDSNVLIFERIKEELATGKSVKSAINGGFDR
                     VWWTIVDTHVASLIAAAFLFQFGTGPIRGFATTLAIGLAANVFTAVFVSRTMFELVLS
                     RRRAATVSI"
     gene            13844..14839
                     /gene="secF"
                     /locus_tag="AC16CY_10475"
     CDS             13844..14839
                     /gene="secF"
                     /locus_tag="AC16CY_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR3354257.1"
                     /translation="MRILTNVNIDWLRWRWHTLALSWLIIVAGLWVIFTRGLPLGIDF
                     SGGTLMVVQFERSVTVGDVRQAIASLPGEEVVQSYGTPDENQILIRLPQAEGATDADS
                     LERGSQQVADALQKAGLPKSEIVKRELVSAVIGADLQRKGIYATIASLVGIMGYIALR
                     FRLSFGLGAMTASIHDVLITLSIMTFAGYELSLNVVAAILTLVGYGVNDQIVIFDRVR
                     ENLRSNRRQPMRDIINLSVNQTLPRTVITAGTVVLAVLSLYLFGGEVLEPFAFAMLVG
                     VITSTYSTVFIASAVAVLLSPRPLASTVAAAPAPASQSPRQAERSGRKAGRVGAR"
     gene            14918..15727
                     /locus_tag="AC16CY_10480"
     CDS             14918..15727
                     /locus_tag="AC16CY_10480"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR3354258.1"
                     /translation="MLPVTYLHAALLGIVQGLTEFLPISSTAHLLIGGRLLGYDDPGG
                     VFTVMIQLGSILAVIWLYRQRIVKVVRGLSSKPEARRFSAMLLVAFLPAVPAGALLAD
                     YVGRVLYETPAVYAWAFIGGGIVMLLVERSARPAVVRSVDDTPVWRAFMIGLAQLAAL
                     VPGVSRSGATIVGGLLMRLDRAAAAEFSFFLAMPTMTAAFVYKLFEVRDQLAPERAGE
                     IAVGFVMAFLAALVVVKPFLRLVGRSGFAPFAWYRIGAGVLILAALRAGWL"
     gene            15724..16359
                     /locus_tag="AC16CY_10485"
     CDS             15724..16359
                     /locus_tag="AC16CY_10485"
                     /inference="COORDINATES: protein motif:HMM:NF016270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR3354259.1"
                     /translation="MIGWFRRSFLAGLFVTVPLVISVAALVWIFGIIDSFVAPLATTA
                     LETALRRKPDPGSLEDYVARFAGLLFTLLLVLVVGAVATNVIGRRLVARAESWLMMIP
                     VFRTIYSPVKQLVAAFSPDNKSGFKRVVMIDDPSRGWVMGFLTREFTVVRDGRTDTMM
                     AVYVPTNHLYLGDVVMYPRERAIFPDLTVEEGVRIFLTGGMSLPARIEGKS"
     gene            16379..18895
                     /locus_tag="AC16CY_10490"
     CDS             16379..18895
                     /locus_tag="AC16CY_10490"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR3354260.1"
                     /translation="MIESSIHPIARVFQAGTPRRYGRYAALLAVAAGLILALAAPAAA
                     QGQQEPASAAATEHRAGGEVNLVLPDLELVDVGGYNGRTLLLGGVFVSIAGLLFGLLI
                     LRQIKNLPVHRSMAEISDLIYETCKTYLVTQGRFLAILEIFIAVIIVLYFGFLSGLEP
                     FRVVIILLFSVVGILGSYAVAWFGIRVNTYANSRTAFASLEGRPHPVYAIPLKAGMSI
                     GMLLISVELLIMLIILLFIPGDYAGPCFIGFAIGESLGASALRIAGGIFTKIADIGSD
                     LMKIVFNIKEDDARNPGVIADCTGDNAGDSVGPSADGFETYGVTGVALIMFISLAVPE
                     AATQVKLLVWIFVMRIMMVIASGASYLINEAIAKVRYGSATRMNFEAPLTQLVWLTSI
                     VSVVLTYIVSNMLIADLGDGTLWWKLASIISCGTLAGAIIPELVKVFTSVESSHVKEV
                     VSSSREGGASLNILSGLVAGNFSAYWIGLSIVTLMSLAYLVSAMGVSMSSLMLAPAVF
                     AFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSVIEGIPGIQQELKKDFGLTVQF
                     DRAKALLEENDGAGNTFKATAKPVLIGTAVVGATTMIFSTIVALTQGLTENTDKLSIL
                     HAPFLLGLVTGGAIIYWFTGASMQAVTTGAYRAVEFIKKHIKLEGVEKASVEDSKKVV
                     EICTQYAQKGMFNIFLTVFFATLAFAFIEPYFFIGYLISIALFGLYQAVFMANAGGAW
                     DNAKKIVETELKMKNTPLHAATVVGDTVGDPFKDTSSVALNPIIKFTTLFGLLAVELA
                     VSLTRDAGPGVSHFLAALFLGVSVIFVYRSFYGMRIGAAR"
     gene            19154..>19742
                     /locus_tag="AC16CY_10495"
     CDS             19154..>19742
                     /locus_tag="AC16CY_10495"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3354261.1"
                     /translation="MPREERMFGDIVDPSIKLGNNQGKSVGVTVLIETTVILAMVIIP
                     LMAADILPTPPSMMAFVAAPPPPPPPPPPPPPPKVEVKPVEIVNPNAAPVEVPQEIKP
                     EPIDTNFDRSVGVEGGIPGGVVGGVVGGLPEAPPPPPPPAAPVRVGGQIKPPTKTKHV
                     NPQYPTIAQSARVQGVVIIEAIIGPNGKVQDAKVLR"
BASE COUNT         3221 a         6416 c         6557 g         3548 t
ORIGIN      
        1 cggggctgat gcatttccac ccgtggtaga cgagattcgg cacctgcacg agcgtcgggt
       61 tctgcgtccc gaggaagaac tcgttgatcg cgccgttggt cggcgagtcc ggcctcgtgt
      121 ccacgagtac cagcttgacc atgcccgcga cgcaggcgaa gttgtccacc tgcaccttgt
      181 ggtagtgcca ggcctttacc acgcccgggt aggtcgccga cacgtagacc tgcccgaacc
      241 gcgtgaagag ttccgcctcg tcggccctga ggatctccat gagccacccg cgctcgtcgg
      301 ggatgagccg cagcggcttc gtcttgacgc cggcaatgcg cgggtgtttg acagaggcgt
      361 tcgtgtacga cactggcgtc gtcatacgcg tcctaccatc gaataccaac ctcggagttg
      421 tcgcccagca tgaagcgata ggcgcttggt ttgaccggtt cccgatagat gcgcacgttc
      481 ttgccgatca ggctgtcggt gacgcgattg gcgagattcc gcagcgagga gccctccagc
      541 acgatgctgt gctcgatctc ggtttcctgg atctcgacgc cgctcatgat cgaggtgaac
      601 gggcccacgt aggcttggac gatcctcgca ccctggccga tgatggcggg tccgcggatc
      661 gtcgagcgct cgacaaccgc cccggcctcg atcacgacct tgccctcgat gcggctctcg
      721 gcatccacgg atccttccac ccgcgtctcg aacgtgtcca ggatcagccg attggcttcg
      781 agcatgtcct cgagcttgcc cgtgtccttc caccagccct cgacgatgtg agggcggacc
      841 gtcagaccat gatcgatcag atcctggatc gcgtcggtga tttccagctc gttccggaaa
      901 cttggcttga tccggttcac ggcgtcgaag acctgcggcc cgaacatata gacgccgacc
      961 agggccagat cgccgatcca ctccttcggc ttctcgacca gccggacgac gtgtccgttg
     1021 cggagctcgg ccacgccgaa catctgcgga tcggggacct tcgtgagcag gatctgggcg
     1081 gccggccgct cccgcctgaa ctcttccacg aaatgcgtga tgcccttgtc gaggaggttg
     1141 tcgccgagat acatcacgaa cggatccgcc ccgatgaagt cccggctgat cttcacggca
     1201 tgcgccagcc cgagcggcgc gtcctgcgcg atgtaggtga cgcgcacccc ccaccgggaa
     1261 ccgtcgccga cggccgcgcg gatctccgcg tgcgtatcgc cgacgacgat gccgatgtcc
     1321 ttgatgcccg catccgcgat ggcctcgatg ccgtagaaga gcaccggctt gttggcgatg
     1381 ggcacgagct gcttggcgct ggtataggtg agcgggcgca ggcgcgtgcc cttgccgccg
     1441 ctgagaatca gtcctttcac gtatcgttac cgctggccgc cgagcgcgcc gaagaagttc
     1501 gagaacgtcc ccagccccgc gagcgtgaac ccgacattga acctcacgtc cgaggggtat
     1561 ccaaacccct cggggtaatc gaacttctgc agctcgagtt gcaggccgca gcactgggcg
     1621 acgtacgagc cggcaaccgt ctgactgacg atcttcgact gcgcgatgtc ccagctgaga
     1681 gaatacgtcc cggtggcgcg cccgtccagc caccgcgacg tcgtcgcggc cgagaggtag
     1741 tccgagccgc tgtaggtcgg cagcgatcga cgatggctgt agttggcgtt caccgacagg
     1801 gtcctcagat tgacgtttcc tcccgcggac agcgtctgca gcccgagccc ggccacgtcg
     1861 tactcgaggc gcaggtttcc gtcgagcgcg acgctcggag acacgcgcgc cgtcagcgcc
     1921 acgggggaca gatcctgcgg ccgtccgtac ccataggtgc tctggtacgt gctgtcatag
     1981 cgcgcggact cgggattgga atagtacgtc tgctgaagac cgacggtgag gaactcccgt
     2041 gtctgtccac gggcctgctc ccgcggcttg ccgcgcgcga agagacggtt ggtcacgccg
     2101 aacgtgaagc gcgtcgaacc gccgaccacg aagtcggaca cgtcgccaac gaccggcgtg
     2161 ctggcgtagt cggcgatctg gctcgtgacg tccacgctga aggccggctc gatgacgtgc
     2221 ttcaaccgct cggcgaacac gctgtcgggg cggtcccaga ttcgcgtcag cacggggccc
     2281 acgatgtcgg agcggaccga catgaagcgg cggtggtagg ggtcttccac ggtgttcccg
     2341 gtcgaatcga cgctgcgcgt gtagtaggtg gtgtgatagc tggcgctggt gttgaccgac
     2401 aggtacgtca gcttcgacaa cggcacgcgc acggtcggcg tgacgtcaac cctcccgtaa
     2461 ccctcgtcgc gcacgacgac tccgtcggtg acggtgcgat ccggcaggaa cgcgtactcg
     2521 gcgttcaccg acgcgtacac cggcgagccg aacagccgct gcggcgcgac gctcgacgtc
     2581 aaccggggcg tgctgccgta gacgtacgag tcggtctggc tcgtgaagag ctcgtttcgc
     2641 tggtagagga cgccgctcga caccggtccg tacgccgcgg tgaggcccgc ttcgatgtac
     2701 cggctgttct gcgacgccat gtagaagttc tgctggtaga gctgctggct gacgatatcg
     2761 gagaagtagt cgaatcgggc ccgcgcgcga atgcgcgggg tgatcgcatg attcatgttc
     2821 ccccggagca cgaagctctg gctggccggc agcgtcgtcg tgatcccgcc gtcggtcacg
     2881 cgaacctcgt tcaggatcag ccgctgcagc cggaggttgc ccgacgactc cggcgcggcg
     2941 acgtatcgat actcggtgcc gagaccgtac cccgaccgcg agaaccagtc gtagaagaac
     3001 gtcgcatcct ggctgcggtt gatggcccag aagaaggcgt tgctgatcgt gtttccctgc
     3061 accctcgagg cgctgtaggt ggggagcagg aagccggtgg cgcggtcgtc ctggatcggg
     3121 tagtacacga cgggcaggta cagcagcggg acgcccttga cgcgaaacac cgtgtttcgc
     3181 gccacggcgt agtcatccac cttcaggacg aagtcgtcgg tcgccatgtc ccatcgcgga
     3241 gtgggctgca cgcaggtggt gaacccgccg cctgaaatgc ggtattcggt gtcgccgacc
     3301 ttttcgatcg tcttccccca gaaatagacc tcggtatcag ggctgcccgc ctgccggatg
     3361 gcgccggcgg cgcccatcgg catcaggccg ctggcgacgt gaaagacagc ggtgttgcgg
     3421 tcgagatcga actcggcgct ctcggcggcg atatgcccgg tccggttcgt gaacaccacg
     3481 ttgcccgtcg cgaccagacg gttggtgtcg aggtacagat cgatctgctc ggcgaagaac
     3541 ttcgtttgtc cgtcccactc ctgctcgacg ccccccgtcc agcgcacatg agtcgccgac
     3601 actatctcca tcggccctcc gaacgagcgc agccgatcga tcagcgagcc tccaggcttc
     3661 ccttgcgtct ctgcggcagg atccggctgc ccgccttgct gcgcgagtgc aggcgccgca
     3721 cccagcagac acacgacgca cgactgaaga atgacgcgag ccatcggctg tcggggtcag
     3781 cgcagaatag cacgctgacg gagacggtcg cgaacggcgc cggccaggaa gatcgacccc
     3841 gcgacgcaca cgacgtcggt cctctcgagc gccgtctcga tcgcgacatt cgggtccggt
     3901 tcggtgcgca cgtcggacgc gcccgccgcg cgcagctgcc tcgccagatc tggggccgga
     3961 atggcgcgag gcgtcggggc agcggtcgcc acgaccgacg acacgacggg cagaagcgcg
     4021 cgcgcgatgt cggcaacgtt cttgtccttc atcacgccga tgaccagcgg cgggcgctcg
     4081 ggatgccacc ggcggaggta gtcggccagc gcacgcgcgc cctcggggtt gtgcgcggcg
     4141 tccagcagca cgcggcggcc gccctcgaga gagaggagct ccaggcgcgc tggccactcg
     4201 gcggcggcga ggccctgctc gacggcggtc ctgggaatcg tcacactgga ttcggcggcg
     4261 gcctcgagca gccggaccgc gacgatagcg ttgccgactt gatgttcccc gcgaaggccc
     4321 agcatgacag ggccgtacga tcccgccggc gtcgtcaagg tgaggcgcgc ccggccgtcg
     4381 accatctccg tctccgcgcg gacgcccgcc atcgcctcga ccagcgtcgc ctctcgctcc
     4441 tcggcgcatc ggcgaatgac ggctgccgcc tcgccggcca ggttgcccat caccaccttc
     4501 atgcgcggct tgatgatgcc ggccttttcg aacgcgatcg ctcccagggt gtcgcccagg
     4561 tgctgctgat ggtcgaagtg aatggtggtg atcgcgcccg ccagcggagc gatgacgttg
     4621 gttgcgtcga atcgcccgcc gagccccact tccacgacgc caacctccac gcccccacgc
     4681 ctgaagagct cgaaggcggc tgccgtcgtc gcctcgaaga aggtcggctg gacgctcagg
     4741 atgccctcgg cgcgaagccg atcggcgcac cgcaatacgt cgtccactgc cgattcgagg
     4801 agcgccgtgt ggacgggctc gtctccgatc acgaagcgct ccgcgagatt gctcaagtga
     4861 ggggatgtgt accgggccgc gcgcaccccg gccgcccgca ggacggcgtg agccatggcc
     4921 gtgacggatc cctttccgtt cgtgccggcc acgtgcagcg agcggaacgc ccgctccgga
     4981 tggttcaggg cctcacacag gcgcgagatg ttgtcgaggc ccagcttgat gccgtaggtc
     5041 tcgagcgcta ggagacgctc gaacacggga cgctcgaaca cgggacctta ggtcgtctcg
     5101 ccggcggcgc cggcgagcgc cggggccggt tcgggggcgg cagatgcggt cgcgcgcatg
     5161 aatcgcagtg cgcgagccag cgtcgctttc agttcgcgac ggtcgacgac gagatcgagc
     5221 attccgtgct cgagcaggaa ctcgcttcgc tgaaatcctt cggggagctt ctgccggatc
     5281 gtctgctcga tcacgcgcgg ccccgcaaac ccgatcagcg ctttcggctc ggcgatattg
     5341 agatcaccca gcatcgcgaa actggccgtc accccccccg tcgtcggatc ggtcaggacc
     5401 gaaatgaatg gaagacccgc ccggtcgagg cgggacaggg ccgccgacac tttggccatc
     5461 tgcatcagcg acagcgcgcc ttccatcatg cgcgcgcccc ccgagcacga gacgacgatg
     5521 gcgggaatgc gccgcgccag cgccatctcg atggcccgcg tgatcttctc gccggcgacc
     5581 acgcccaggc tgccgccgat gaacgtgtac tccatggcgg cgacgacgac gtcgaggccg
     5641 tcgagctggc cggtcgcgac gatcaccgaa tccttgagtc cggtggcctc catcgtcttc
     5701 tggagccgct cgcggtaggg cttggtatcc gtaaagcgca gcggatcggt ggacgtcagg
     5761 tccgggaagt gttcctcgta ctggccctcg tcgaggagcg ccgccagccg ctcagcggct
     5821 ctgaggcgga agtgatgcga gcacttcggg cacacgctga ggttggaatc gaggtccttc
     5881 ttgaagatca gctgggagca gtcaggacat ttgacccaga gcccttcagg aacccggctc
     5941 gggttctcgg ccgtggagga ggcgatgggc ttccgggtct tcttgaacca ggccatcggg
     6001 gggactttag cacggccgca gggagtcgcg ttcaggcccg cgggacgtag tactgcgtgc
     6061 cccgtcccat cgcgtttatc tgacggatga ggtcgtcgag ggcctcgtcg cttccggcga
     6121 ggtcgaattg cgaggtctcc accaccagga gcggcgtggc cgagtagtga aagaagaagt
     6181 gctggtacgc ctcgttcagc tcgcgcaggt attcgacgct cgggtcgagc gtctcaccgt
     6241 ccgttttcgc cgcgcgatcc ttcagccggc ggacgaggac gtcggtcggg gcctggagat
     6301 aaatgacgag atcgggtgag ggcacgtccg ccgccagcag gtcgtacagc cgctgataaa
     6361 tgaacagctc gttgtcgtcg aggttgaggt aagcgaagat cttgtccctg tcgaagaggt
     6421 agtcgctcac cgtcgtctgc gcgaacagat caccttgccg catcgacagc tgctgccggt
     6481 ggcgattgag caggtagaag agctgcgcct gcagagccgc acccggacgg cccgcgtaga
     6541 aatcgtcgag gaacggattc tctctctcct cgaacagcgt cgccgcgtcg agacgcacgg
     6601 ccagacgatc agccagcgag gtcttgccga cgccgatggg cccttcaatg gcgatgtact
     6661 gaaacgacat gcatgacggc tggggtgcgc cggatccgct gcatgctatc ttaacgatcg
     6721 acccgatgac caatgcgcaa tgcgcgattg ttgctttgtg ccttgttgtg accgcctgcg
     6781 cgtcggcgcc ggtcgcgccc attgccgcac ccgctgcctt gcctcccgcg cctgtcgttc
     6841 cggtcacgac ctgggaagag aagattggct ggatccttcg actcgaggat gagcgtctca
     6901 tccgcgaccc gaacccgccg cccccgcgtg tcattcaggc tgcgacgtcg cgtctcccgg
     6961 aaatcctcgc cccacctccg ccgtcggatc tgatcctgct gctcggcgat caacaggccc
     7021 gcgtgcgccg tcgcgccgcg ctcgcgctcg gccgcgtcgg tcttgctgaa gcggtcgagc
     7081 cgctcgcggg cctgctcacc gacgaggagt tcgaggtgcg ccagatggcg gccttcgcgc
     7141 tgggcctggt gggcgagtcg gctgcgaggc ccgcgctgct ggcggcgctc aacaacgacc
     7201 cggagctggt cgttcagggg cgggcggctg aggcgctcgg ggccatcggc gatcgcgcgg
     7261 acgccgacgc catcgccgcg atggtccagc ggcaggtgtc ggcgggcgtg ctgggaggcc
     7321 tcgaccctga cgacatcggc tacccgaagg ccggcggagt cgaggcggtc cggctcggcg
     7381 tgtacgcgct ggcgcggctg agagcctacg atgcgctggc gggtgccatc ctcgacgcgc
     7441 agggcaaacc cgtctcgtcg tggtggccga tcgcgtatgc gttgcaacgg tgcggcgacg
     7501 accgcgcggc gccggccttg atggccctgc ttccgacgcc aggacgcttc accgcgtcct
     7561 tcgccatccg cggcctcgcc gcgttcaagg ccgttcacgc cgccgacgcc cttcgagaga
     7621 tcgtgaccga gcgcacgcgc gatcccgcca tcgtcattca atcggtccgc gccttgtcgc
     7681 agttcggcga cagggcatcg gttcccgtcc tgaccaggat gctggcggat cggtctgtga
     7741 caggagcgct cgagctcgag gtgatgtcgg cgctcggaaa cctggcggac gagagcgaca
     7801 cggacgtgct gctcgaactc gtctccgatt cgaagccctc cgtgcgcgca acggcgttcc
     7861 gcgcgctggc tcgcgtcgag ccggcgacgt ttctggccgc cctggcaggc ctggacgcgg
     7921 atcccgactg gactgtccgc gtcgcggtgg cgaacgcgct cggcggtctg ccggcggaac
     7981 aaagcatccc caagctgaat cagatgctgc aggatcgcga cggcagggtg attccggccg
     8041 tgctgacggc gcttggggct accaagtcgc ccggcgtgga ccggctgctc ctcgatcgcc
     8101 tgcgctccga cgacttcatg gtccgcgcaa gcgctgccaa tgcgcttgcg gagatgaagg
     8161 cggccggcgc cgccgggccg ctcgcgcagg cataccgcgc ggctgacggc gatgcgacct
     8221 acgtggcgcg cgcggccgtg ctgagcgccc tctcgcggct cgacccggcg gcggcacgtc
     8281 cggtcctgga ggacgcgctg aaggatcgcg attgggcagt ccgcgtgcgc gccgtcgctc
     8341 tgctccggga acagggcgca accgggctgg accaggcgat tcggcccgcc cctgccggcc
     8401 gcgtcgtgca ggaggcggaa agacaggcgc tcgcgacgcc acagttctct ccgcacgcct
     8461 tcatcgagac cgacaagggg acggtggaaa tcgaattggc cgtcctcgat gccccggaaa
     8521 cggtgggcaa cttcatgtca ctggcccgga agggattttt caacgccgtc gccattcatc
     8581 gcgtcgttcc cgatttcgtg gtgcaggacg gggatccgcg cggcgatggc gaaggcggac
     8641 ccggttacag cattcgcgac gagatcaatc agcggccgta tctgctcggg acggtcggca
     8701 tggcgctcga ctgggaagac accggcggga gccagttctt catcacgctc tcaccgcagc
     8761 cgcacctcga cgggcggtac accgtcttcg gacacgtggt gaacggcatg gacgtcgcgg
     8821 accggctgct gccgggcgat gtcgttcgtc gtgtgaggat ctgggacggt gtgaaggaac
     8881 agtaggggct gctccaactc ccaactccca gatgactgga agttgggagt cgggattagt
     8941 ggcgggctat ctcttcttcg ccgccttctt cttcgccttc tttgccatgg ttgtaatccc
     9001 ccctttcgct ggttggattg cgatttgctc ctcgccatcc agcgcactct gccgcctggc
     9061 acgtcggccc ggatgacggt catttcgcct tgaggctcac accaacgcga cagcgtcagg
     9121 agagcagcaa ctcagagcca aaaaaaaatg gggtgctcca tcgagcaccc cgtgaagctg
     9181 cggaaaacct accgcttctt cgcggccttc ttcttggcct tcttcgccat tgttttctcc
     9241 tcaggattag cggctcagcc tccgacggac cgcttggacc gtcacaacgg gtgggctgag
     9301 ccatccaccc tcataccaga tgtagattat gggtgagatt tggctgctgt caagcacaaa
     9361 atcgttcttc ttgtcaaaag atcgcgtcga cgcgataccc gacgacacgc tcgattgcac
     9421 ccatcatgtc aagtgtcggt tgaagtgcgc gagtgctgca ctccaactgc aacggtgcga
     9481 gggtgcgagc gtgcgatggt gcggtgttct aggttcgcga cgtgcgaagg cgctgctttc
     9541 gttcgtggcg ttcgagcgcg agggtcacga gcttgtcgag caggtcggga tacgcgagac
     9601 ccgtggcttc ccacatcttc gggaacatcg agatggccgt gaacccgggg atggtgttga
     9661 cctcgttcag gtagagaccg ccgccgcggt cgtcgagcag gaagtccaca cgggccatgc
     9721 cgcagagatc gagcgccttg aaggcctcga cgctgaggcg gcgaacccgg tcggtcagct
     9781 cgggagacag atccgccgga atcttcgtcg ccgcgccgtc gggatccagg tacttcgcct
     9841 cgtacgaata gaagccgtct cgatgcgtca cgacgacctc acccggaaga gacgcctgcg
     9901 ggtcgtcgtt gccgagcacg ccgcactcga tttcccgcgc atgcggcacg gccgcttcga
     9961 cgacgacctt gcgatcgaac tccagcgcaa gtgacatcgc cgcgcccagt tccgtgtcgg
    10021 cgtccgcctt cgagatcccg acactcgagc cgagattggc gggcttgacg aagaccggga
    10081 aacgcagttt ccgggcgatt tccgctgaaa tcttccccga atcggcctcc cattcatggg
    10141 ccaggatcgt cacgtgcgga gcgagcggca ggccgtgggc ggcgaagacg gctttggcca
    10201 tcgccttgtc catcccgagc gccgagccga ggacgccggc gccgacgtac ggaacgttgg
    10261 ccagttccag caggccctga acggtgccgt cttcgccgta cggcccatgc agcactggaa
    10321 agaccacgtc aacagcgccg ctgacggccc tggtgggttc ggcgacctga agtgcgcctc
    10381 cggcggggtt ctcgtcctcg acctcggctg ccgtgagcgc cgacggttcc ctggttccga
    10441 gcagccaccg cccgtcgcgg gcgatgcgga ttggcacgag gtcgtatcgg ctgcgatcga
    10501 ggtgccggaa gatcgacgcg gccgacacga ccgacacctc gtgctcgccc gatcgcccgc
    10561 cgtagatgac gccgacgcgg attttcatcg cgcccataat aatggggtcg ggagtcattt
    10621 cttgaggaca cttccaagga tcagtacctg tgcggggagt gtcctcgaga aatgactccc
    10681 gacccctttc gcttcgagct gctccgcacc gacggagctg cccgcctcgg cgaggtccat
    10741 acgccgcacg gcgtcgtcca tacgccggcc ttcatgcccg tgggcacgca gggctcgatc
    10801 aagggcgcgc tgtttcgcga cgtggaggac gccggcgccg agatcgtgct cggcaacacc
    10861 tatcacctct atctcaggcc cggtgacgag cgcatatgca ggctgggagg gttgcatcgc
    10921 ttcatcggct ggaaccggcc gatcctgacc gacagcggcg gctttcaggt gttcagcctc
    10981 tccgagcggc ggcggatcac cgaggacggc gccgagttcc gctcgcacct cgatgggtct
    11041 ctgcatcacc tctcgccgga acgatcggtg gacatccagg cgcggctcgg gtcggacgtg
    11101 gcgatggtgc tcgacgagtg ccttgcgtac ccgtcaacac atgaacagac cgccgaatcc
    11161 gtggggcgat cgcttcgctg ggccgaacga tgccgacgcc ggctggaggc actgcggtcc
    11221 tcgccgccgc ccgatgtggc cgtgatgaat cccggccagg cgcagttcgg gatcgtgcag
    11281 ggcggtgtgt ttgccgacct ccgtcgtgag agcgccgaag cgacgatcgc gatcggcttc
    11341 gacggttacg cgatcggcgg cctcagcgtc ggggaaccgg ttgacgtgat gtacgacatg
    11401 gtcgagggta ccgcgcgcca tctgccggag cgcgagccga gatacctcat gggcgccggg
    11461 acgcccgagg atctggtcga gtgcgtggga cggggcatcg atctgttcga ctgcgtcctg
    11521 cccacgcgca acgcgcgcaa cggacagctc ttcacgagcc agggccggat caacatcaag
    11581 aacgcccggt atgccgacga cgacgcgccg ctcgatgcca actgccggtg ctacacgtgc
    11641 cgccgtcatt cgcgggcata tctccggcac ctgtaccttg cgggcgagat gacggccggc
    11701 gcccttaaca cgttgcataa tctgaccttc taccttgaca cgatgaaacg gatcagggag
    11761 gctattgtgt tcgggcgctt cgacatgttc aggcaggagt tccttcagtc ggttgcccgc
    11821 ctgtcttacg aatcatgaat cttctcgatc cttttctcgt ggccgtggcc ccgcagggga
    11881 gcccgagccc gtggcttcag atcgtcccgt tcatcctgat tctcgggatc ttctatttcg
    11941 tgatggtgct gccgatgcgg aagcgccagc agaaggttca agcgtttctg gcggcgctga
    12001 aggttggcga caaggtcatc acgtccggcg gcatattcgg gtcgatcacg cgcgtgagcg
    12061 accaggcagt gcagctccag atcgccaaca acgtgcgcat cgacgtgtcc cgccaggcca
    12121 tcgtcggcta ccaggggcag gagcccgtgg ttcctgaggg caaccagcaa tgatcaagaa
    12181 tctccagtgg cggctcggga tcatcgccgt cgtcatcggc ctctcggtgt gggcgttcgt
    12241 tccccctcgg gaaaaggtga agctgggtct cgacctgaaa ggcggcgttc acctcgtcct
    12301 gcgcgtcaag accgacgacg ccctgcgcgt cgagacggag acgacggtcg aacggcttcg
    12361 agactcactt gcgcgggagg gcgtgcaggt ctcgaaggtc gaagtgacca gtcccaccga
    12421 gtttcgagtc gaaggcatcc aggacgatgc cgggttccga gcgcaggccg ccgccgatgc
    12481 cgagtcggtc ttcgaccgct cgcccgaggc ggggggatac acctaccgga tcaagcccaa
    12541 cgtcgcgaac cagcttcgcg atcagaccgt ggcccaggca ctcgaaacga tcgagcgtcg
    12601 cgtcaacgag ctgggcgtgg ccgagccgat cgtggcccga tacacagagc aggatcagat
    12661 cgtcgtgcag ctcccgggcg tgagcgacgt ccgccgggcg aaggacatca tcaagtcgac
    12721 ggcccggctc gagttgaggc tcatggacca ggggcccttt ccaagccgcg aggcggccct
    12781 gcaggcggtc aacaacgcct tgccaccgga cgcggagatc cttccaggac gcagcgaggg
    12841 aaccgggacg gcgggcacgg ccggcacctc gtactatctc gtgaagagag tggcggccgt
    12901 cgcgggaaac gacctgcgca acgcccagca gtcgctcgac gagtacaacc ggcccgccgt
    12961 tgcgttcacg ctgaagcagg acgcggcgga gcgtttcggc aagttcaccc agtcgaacct
    13021 cgggcgcccg atggcgatcg tgctcgacga gcgcgtcatg tcctacgcca cgattcaagg
    13081 gcgcatcacg gacaacggac agatcaccgg catcactcgc gacgaaatgc tcgatacggt
    13141 gatcacactg aagtccgggg cgctcccggc gtcgatggac tacctcgaag agcggacggt
    13201 gggagcctcg ctcggcgagg actcgattcg gtcgggcgtc acggccgccg tgggcggcct
    13261 gctgctcgtc atggtcttca tgctcttcta ctacaagctc gcgggcctca acgcgctcac
    13321 ctccatcatc gtgaacctga tcatcctgct gggactcatg gccagcatag acgcgtcgat
    13381 gacgctgccg ggctttgcgg gcttcatcct gacgatcggg atcggcgtgg actcgaacgt
    13441 gctgatcttc gagcgaatca aggaggagct ggcgacgggc aagagcgtga aatccgcgat
    13501 caacggcgga ttcgaccgcg tctggtggac gatcgtcgat acgcacgtcg cctcgctgat
    13561 tgcggcggcg ttcctcttcc agttcggcac cgggccgatt cgcggctttg ccacgacgct
    13621 ggcgatcggg ctcgcggcca acgtcttcac ggccgtgttc gtgtcgcgga ccatgttcga
    13681 gttggtgctc tcgcgccgcc gtgcggcgac cgtcagcatc taatgggctg tatcaactgg
    13741 aagagtaccg cgaacaacga gcttcgaacc gcgatttccg gccgcgacaa cggagcggcc
    13801 ggcagtaatg ctcgaatgac ataccactag tgggcgaatc atcatgcgca tcctgaccaa
    13861 cgtcaacatc gactggctcc gctggcgctg gcacacgctg gccctgtcgt ggctcatcat
    13921 cgtcgccggc ctgtgggtga tcttcacgcg cggcctgccg ctcggcatcg atttctccgg
    13981 cgggaccctg atggtggtcc agttcgagcg gagtgtcacg gtgggcgacg tgcgccaggc
    14041 catcgcctcg cttccgggcg aggaagtcgt gcagtcgtat ggcacacccg acgagaacca
    14101 gattctgatt cgcctgccgc aggcggaggg cgcgacggac gccgacagcc tggaacgcgg
    14161 atcgcagcag gtcgccgacg ccctccagaa agcggggctg ccgaagtccg agatcgtcaa
    14221 acgcgagctc gtgagcgccg tgatcggcgc ggacctgcag cggaagggga tctacgcgac
    14281 gatcgcgtcg cttgtgggca tcatgggcta catcgccctg cgcttccggc tcagtttcgg
    14341 actcggcgcg atgacggcga gcatccacga cgtgctcatc acgctctcga tcatgacgtt
    14401 tgcgggctac gagctgtcgc tcaacgtggt cgcggccatc ctgacgctgg tcggctacgg
    14461 cgtcaacgac cagatcgtca tcttcgatcg cgtgcgcgag aacctccgct cgaaccgccg
    14521 gcagccgatg cgggacatca tcaacctcag cgtgaaccag acgctgcccc ggaccgtgat
    14581 caccgccggg accgtcgtac tggccgttct gtcgctctac ctgttcggcg gcgaggtcct
    14641 cgagccgttt gcgttcgcga tgctcgtggg ggtgatcacc agcacctact cgacggtgtt
    14701 catcgcctcg gcggtcgccg tgctgctcag cccgcgtccc ctcgcctcca ccgtggccgc
    14761 ggcgccggcg ccggccagtc agtctccgcg gcaggcggaa cgatcgggcc gtaaggcagg
    14821 gcgcgtgggc gcacgctagt cggcggcagg ccggctcccg cgggccggcc gctccgcgcg
    14881 cgactgcgcg accaatgccg caacggccaa ccgctgactg ctgcctgtga cgtacctcca
    14941 cgccgcgctg ctcggaatcg tgcaaggcct gaccgagttc ctgccgattt cctccacggc
    15001 gcacctcctc atcggggggc ggctgctggg gtatgacgac cccggtggcg tcttcacggt
    15061 catgattcag ctcgggtcga ttctcgccgt gatctggctc taccggcagc ggatcgtgaa
    15121 ggtcgtgcgc ggcctgtcct cgaagcccga ggcccggcga ttctcggcga tgctgcttgt
    15181 cgccttcctg ccggcggttc cggcaggggc cttgctggcc gactacgtcg ggagagtgct
    15241 gtacgagacg ccggccgtgt acgcctgggc gttcatcggc ggcgggatcg tgatgctcct
    15301 cgtggagcgg tccgcccgcc cggccgtcgt ccggagcgtg gacgacacgc cggtgtggcg
    15361 cgccttcatg atcggtctcg cgcagctcgc tgccctcgtc ccgggagtgt cccgatcggg
    15421 cgcgaccatc gtcggcgggc tgctcatgcg cctcgaccgg gcggccgcgg ccgagttctc
    15481 gttttttctc gcgatgccga ccatgaccgc ggcgttcgtg tacaagctct tcgaggtgcg
    15541 cgaccagcta gcgccggagc gcgccggcga aatcgccgtc ggcttcgtga tggcgttcct
    15601 ggcggccctg gtggtggtga agccgttcct tcggctggtc ggccgctcgg ggttcgctcc
    15661 ctttgcctgg tatcgaatcg gggcaggcgt gctgattctc gccgccctgc gggcgggctg
    15721 gctatgattg gctggtttcg gcgcagcttt ctggcgggcc tcttcgtaac ggtgcccctg
    15781 gtcatcagcg ttgcggccct ggtctggatc ttcggtatca ttgatagttt cgtggcgccg
    15841 ttggcgacga cagccttgga gacggccctg cgccggaaac ccgatccggg ctcgctcgag
    15901 gactacgttg cgcggttcgc cggtctgctg ttcaccctgc tcctggtgct ggttgtcggg
    15961 gccgtcgcga ccaacgtcat cggtcgccgg ctggtcgcga gggccgagag ctggttgatg
    16021 atgatcccgg tgttccggac gatttattcg cccgtgaagc agctcgtggc ggcgttctcg
    16081 ccagacaaca agtccggctt caagcgcgtc gtgatgatcg acgacccgag ccgcggctgg
    16141 gtcatggggt ttctgacgag ggagttcacg gtggttcgcg atggtcgcac cgacacgatg
    16201 atggcggtct acgtgccgac gaaccatctg tacctgggcg acgtcgtgat gtacccgcgc
    16261 gagcgcgcga tcttccccga cctgaccgtc gaggaaggag tgcggatttt tcttaccggc
    16321 gggatgtcgc tgcccgcccg gatcgaaggc aagtcgtaag agaggagcag acagagagat
    16381 gatcgagtcg tcgattcacc ccatcgcgcg agtgttccag gcggggaccc cacgaagata
    16441 cggccggtat gcggccttgc tggcggtggc cgccgggttg atcctcgcgc tggcggcgcc
    16501 ggccgcggcg caaggacaac aggagcctgc gtctgcggcg gcgaccgagc atcgcgccgg
    16561 cggcgaggtg aacctggtgc tgccggacct ggagctggtc gacgtcggcg gctacaacgg
    16621 ccggacgctg cttctgggtg gcgtgttcgt atcgatcgcg ggtctgctgt tcgggctgct
    16681 catcctcagg cagatcaaga acctccccgt gcaccgctcg atggcggaga tctccgatct
    16741 catttacgag acgtgcaaaa cgtatctcgt tacccagggt cgcttcctcg cgattctgga
    16801 gattttcatc gcggtcatca tcgtcctgta tttcggcttc ctgtccgggc tcgagccctt
    16861 ccgggtcgtc atcatcctgc tcttcagcgt ggtcggcatc ctgggcagct acgccgtcgc
    16921 ctggttcggc atccgggtca acacgtacgc gaactcgcgg acggcgttcg ccagcctcga
    16981 aggccggccg caccccgtgt acgcgatccc gctcaaggcc ggcatgagca tcggcatgct
    17041 gctcatcagc gtcgagctgc tgatcatgct gatcatcctg ctgttcattc ccggcgacta
    17101 cgcgggaccc tgcttcatcg ggttcgccat cggcgagtcg ctgggcgcct cggccctgcg
    17161 catcgcgggg ggcatcttca cgaagatcgc cgacatcggg tcggacctga tgaagatcgt
    17221 cttcaacatc aaggaggacg atgcgcggaa tcccggcgtg atcgccgact gcacgggcga
    17281 caacgcgggc gactcggtgg ggccgagcgc ggacgggttc gagacctacg gcgtcaccgg
    17341 cgtggcgctc atcatgttca tttcgctggc ggtgccggaa gcggccacgc aggtgaagct
    17401 gctcgtctgg atcttcgtga tgcgcatcat gatggtcatc gcgagcggcg cgtcgtacct
    17461 gatcaacgaa gccatcgcca aagtgcgcta cggaagcgcg acacggatga acttcgaggc
    17521 gccgctcacg cagctcgtct ggctgacctc gatcgtctcg gtggtgctga cctacatcgt
    17581 gtcgaacatg ctcatcgcgg acctgggcga cggcacgctc tggtggaagc tggcgagtat
    17641 catctcgtgc ggcacgctgg cgggcgcgat catccccgag ttggtgaagg tgttcacctc
    17701 ggtggaatcg agccacgtaa aagaggtcgt cagctcatcg cgcgagggcg gggcgtccct
    17761 gaacattctc tcgggcctgg tagccggcaa cttcagcgcc tactggatcg gcctgagcat
    17821 cgtcaccctg atgtcattgg cctacctggt cagcgcgatg ggcgtctcga tgtcgagcct
    17881 gatgctggcc ccggcggtgt tcgccttcgg gctggtggcg ttcggcttcc tcggcatggg
    17941 acccgtcacg attgccgtcg actcatacgg cccggtcacc gacaacgccc agtcggtcta
    18001 cgagctgtcg gtcatcgaag ggattcccgg catccagcag gagctgaaga aggacttcgg
    18061 gctcaccgtg cagttcgaca gggcgaaggc gctgctcgag gagaacgacg gcgcgggcaa
    18121 cacgttcaag gccaccgcca agccggtgct catcggcacg gcggtcgtcg gcgcgacgac
    18181 gatgatcttt tcgacgatcg tcgcgctgac gcaggggctg acggagaaca ccgacaagct
    18241 ctcgatcctc catgcgccgt tcctgctcgg cctcgtgacg ggcggggcga tcatctactg
    18301 gttcacgggc gcgtcgatgc aggcggtgac gacgggcgcc taccgggccg tcgagttcat
    18361 caagaagcac atcaagctcg aaggcgtcga gaaggcgtcg gtcgaggaca gcaagaaggt
    18421 ggtcgagatc tgcacgcagt acgcgcagaa gggcatgttc aacatcttcc tgaccgtctt
    18481 cttcgccacg ctggcgtttg ccttcatcga gccctacttc ttcatcgggt acctcatctc
    18541 aatcgcgctc ttcggcctgt atcaggcggt cttcatggcc aacgccggcg gcgcgtggga
    18601 caacgcgaag aagatcgtcg agaccgagct caagatgaag aacacgccgc tgcacgccgc
    18661 gacggtcgtg ggggatacgg tcggggaccc gttcaaggac acctcgtcgg tggcgctcaa
    18721 ccccatcatc aagttcacga cgctcttcgg gttgctggcc gtcgagttgg ccgtaagcct
    18781 gacgcgggac gcgggccccg gagtcagcca cttcctggcc gcgctgtttc tgggcgtgtc
    18841 ggtgatcttt gtgtaccgct cgttctacgg aatgcgcatc ggcgcggcga gataatcgag
    18901 tgttcggagt cgcgcgcgac ggcgccgttt gtcgcccttg taacggactt ccggcgcggc
    18961 actttcgggc ttgcgttgga tgagttccgg gatagaatgc cgcctctcgc gatcaggggt
    19021 acggagcaca ttggggaaac cggtgtctaa gctagtgctg ggcgatccag gtggatctcg
    19081 cggttcgttg acaccaattt ttgggcctag ctataatcgg ccgccccgcg gggattccag
    19141 ggagggatga ccggtgccac gcgaagaacg catgttcggc gatatcgtcg atccgtccat
    19201 caagctcggg aacaaccagg ggaagtcggt cggggtgacg gtgctcatcg agacgaccgt
    19261 cattctcgca atggtcatca ttccgttgat ggcggccgac attctgccga cgccgccgtc
    19321 gatgatggcg ttcgtcgcag cccctccgcc gcctccaccc ccacccccac caccgccgcc
    19381 gccaaaggtt gaagtcaagc cggtcgagat cgtcaacccg aatgcggccc cggtcgaggt
    19441 accgcaggag atcaagccgg aaccgattga cacgaacttc gatcgcagcg tcggcgtgga
    19501 agggggaatc cccggcggcg tcgtgggcgg cgtcgtaggc ggcttgcccg aagcgccccc
    19561 gcctccgcct ccgccggcgg cgccggtccg cgtcggcggg cagatcaagc cgccaaccaa
    19621 gaccaagcac gtcaacccgc agtacccgac gatcgcgcag tcggcgcgcg tgcagggtgt
    19681 cgtcatcatc gaggccatca tcgggccgaa tggcaaggtc caggacgcga aggtcctgcg
    19741 tt
//
LOCUS       DBNGSH010000452         7464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_212008, whole genome shotgun sequence.
ACCESSION   DBNGSH010000452 DBNGSH010000000
VERSION     DBNGSH010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_212008"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..946)
                     /locus_tag="AC16CY_10500"
     CDS             complement(140..946)
                     /locus_tag="AC16CY_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3354262.1"
                     /translation="MRTSLLLIALVAGASTGAAQQPQVPFEPTVGQAGKDVVWVPSPE
                     VTVEKMLEVGKVTAQDFAIDLGSGDGRNVIAAAKRGARGLGVEYNPDMVALSKRLAAQ
                     AGVADRAQFVEADMFTADISKADVMLLFLLPSNLLRLRDTLFNLRPGARIVLNTFGIQ
                     DWEADETFTVEDCTQWCTVYLIIVPAKVAGNWRIGSDTLELKQEFQKVTGTLTSGGQS
                     VPVTGSLRGTEISLKAGTREITGQVRGNEISGNAKNGSATSSWRATRAGS"
     gene            complement(950..1633)
                     /locus_tag="AC16CY_10505"
     CDS             complement(950..1633)
                     /locus_tag="AC16CY_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3354263.1"
                     /translation="MPAVSETALARHHDDGLASAPRTLTSAPTRAEREAAAIELRRAF
                     CEELRAARESRGISLLRIAEVTKVSESLFAELERGHLSRWPTGIYRRAFFREYAVHVG
                     LPAESTLSEFIRLFPEDRDSDTSRDALVPGPLRLTMPRPTWRQLSTIYFQTAVLDLVI
                     VLLGSGALSWLTAVGPWISIAIVALGYHATGTIVLGCSPVAWWARGRLHRKLDKQPAR
                     PINLFPQDE"
     gene            complement(1716..2987)
                     /locus_tag="AC16CY_10510"
     CDS             complement(1716..2987)
                     /locus_tag="AC16CY_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354264.1"
                     /translation="MQVPVPNHMFNIRILAGVLAWAGSLVVLSGDVHPSLLRNHPAIA
                     YATTPVTDAVARLNGRLQRGEVALDRGGPSGYLKAVLDALDVPIESQVLVFSKTSFQA
                     TKIGPANPRAIFFNDTVAVGWVRSGPVLEFVSHDPKQGAIFYTLEQTHTAVPQFERND
                     ACVMCHTSDATHNVPGMFVGSVFPSVDGTTMYGPAFTTDHRSPFELRWGGWFVTGSHQ
                     ASRHMGNAVATDPTDLGAMITPESVHVARLDGRFDMTGYLSPHSDIVALLVLEHQVQM
                     LNLITRVGWEARVGAEARRPLEAAAAELVDYLLFVDENPLPGPISGPTAFAKAFGARG
                     PRDSKGRSLRDLDLERRLLKYPCSYLVYSEPFDGLPPSAKAAVYRRMWDVLSGRETGD
                     RYSTLSRADRTAIIDILRETKADLPEYWAVP"
     gene            complement(3113..4138)
                     /locus_tag="AC16CY_10515"
     CDS             complement(3113..4138)
                     /locus_tag="AC16CY_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HIR3354265.1"
                     /translation="MRKRPLPDTWPDLDDEALLDVRLSDLPLEIEGTLAARIEQIREE
                     LRARGLGFPLHFYLSDEWFTPDGSTAVAIPFYLAHPRLENLERKQMLEVEGGEHEWCM
                     RILRHEAGHAIDNAYRLRRRQKRRQIFGSPRKPYPEFYTPKPYSKSFVLHLDAWYAQS
                     HPDEDFAETFALWLTPNSEWRPRYTGWKALTKLEYMDALMSSLRDVPPPFQNRNEVDP
                     LTAIRTTLRQHYKNKRRHYGVDYPNFYDRDLRRLFSDAPEFSSHPTAAQFISRIRPTV
                     RKAVAGWTGIYQYTIDKVLEDMMRRCRELKLRLAVPEEHARQEFTVLLTVQAMNYLHS
                     GGHRLFL"
     gene            4190..6058
                     /gene="pepF"
                     /locus_tag="AC16CY_10520"
     CDS             4190..6058
                     /gene="pepF"
                     /locus_tag="AC16CY_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIR3354266.1"
                     /translation="MRAVWAGLAVIGMTVLMTGQERDRAKIAQDYKWNVAEVYPDDAA
                     WRARKEALTKELSSVRGFQGKLGSSPATMAEALETMSRLDKELARLYVYASMRSDEDT
                     RVSLTQGMQQEMQQIAAKFGAEASFIEPEVLKVGSASIEEAIASERRLAPYAFYLRDI
                     ARREPHTLSDPEEKLLADVAPLAGSPTNIFTILSNADFPYPTITLADGRQAKVDQAGY
                     TELRTAPSRADRQAAMSAFFGALGAFSRTLGVTMNSSVQKTLFYSRARKHSSNLEASL
                     NGPNVPVSVYTRLVEGVNRHLPTFHRYLRLRKRMMGLTDDLHYYDLYAPLVASVDLRY
                     TPEEAQQHVIGAMAPLGTDYVGVLRRAFRERWIDWYATEGKVSGAYSNGAVYDVHPYM
                     LLNYLGQYNDVSTLAHELGHTMQSYYSNKVQPYPTASYPTFVAEVASTFNEALLIDYM
                     LKQIKDTPTRLSLLGNYLEGIKATLFRQTQFAEFELRMHEMGQKGEPITGEGLARLYL
                     DITRKYYGHDQKVTVVDDYIAHEWSYIPHFYRDFYVFQYATSFTAAEALSAKVLAGDQ
                     SATQRYLTFLSAGGSKYPIDLLKDAGVDMTTDEPLDLAVKRMNVVMDEMEKLIASR"
     gene            complement(6116..7024)
                     /gene="rocF"
                     /locus_tag="AC16CY_10525"
     CDS             complement(6116..7024)
                     /gene="rocF"
                     /locus_tag="AC16CY_10525"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000050 - urea cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HIR3354267.1"
                     /translation="MKPVHIIGVPLDLGAGRRGVDMGPSAFRIAGLGPQIAALGRTVS
                     DLGDVPAPIAETRPETDPRKKYVREIAGVCAGVYSAVAGALDAGAVPLVLGGDHSLAA
                     GSVAASADWVRRMTSYPMGLIWIDAHGDMNTPETTTSGNVHGMPLATLLGQGPEELAS
                     IGASPSVLADRTVLVGIRNLDEREKDRIRESGVHVFTMKDIDRHGIAVVAEKAIALAS
                     AGTGGIHVSFDLDACDPAVAPGVGTPVRGGLDYREAHMVMELVADSQRLVALDLVEVN
                     PTLDIRNTTAEFGAELALSALGKGIL"
     gene            complement(7120..>7464)
                     /locus_tag="AC16CY_10530"
     CDS             complement(7120..>7464)
                     /locus_tag="AC16CY_10530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen/coproporphyrinogen oxidase"
                     /protein_id="HIR3354268.1"
                     /translation="PDGHVLIRGFLGGGRDPGRLDGSDEELVETARREMHELLGISGS
                     ALLTRVFRWTRQSPQYETGHLQRVAAIERRLGGLPGVFVTGSGFRAIGIPDCISDGRA
                     TAVRAVEYLASL"
BASE COUNT         1259 a         2419 c         2499 g         1287 t
ORIGIN      
        1 ggctgcacag ccggctcagc gcatctcttt gactgtgaag atcttcgtcg ccgtcgacgg
       61 atccgtcatt ccttgacgcc ggctggaata aaggcggtac ttgcccagac ttccagccgg
      121 tgccgcccca ggtgtgtgat cagctgcctg cgcgggtcgc gcgccaggag gacgtcgcgc
      181 tgccgttctt ggcgttccca gagatctcgt ttccacggac ttgaccggtg atctcccgcg
      241 tcccggcctt gagtgagatc tccgttccac gaaggctgcc agtgacgggt acggactgtc
      301 cgcctgaggt cagcgtccct gttaccttct gaaattcctg cttcaactcg agcgtgtcgc
      361 tcccgattcg ccaattgccg gccactttgg cgggcacgat gatcaggtag accgtgcacc
      421 actgggtgca atcctccacg gtgaacgtct cgtcggcctc ccagtcctga atgccaaagg
      481 tattcaggac gatgcgggcg ccggggcgga ggttgaacag cgtgtcgcgc agccggagca
      541 ggttgctcgg cagcaggaac agcagcatca cgtcggcctt cgagatgtcg gcggtgaaca
      601 tgtcggcttc gacgaactgc gcacggtcgg cgacgccggc ctgtgcggcc aggcgtttgg
      661 acaacgcgac catgtccggg ttgtactcga cacccagccc gcgggcgccg cgcttggcag
      721 ccgcaatgac gttgcggccg tcgccggagc cgagatcgat cgcgaagtcc tgcgcagtga
      781 ccttcccgac ctcgagcatc ttctcgaccg tcacttcggg cgacggcacc cacacgacgt
      841 ccttgccggc ctgtccgacg gtcggttcaa acggaacctg cggttgctgc gcggcgccgg
      901 tactcgcgcc ggcaacgagg gcaatcaaga gcagtgatgt gcgcatggtc tattcgtcct
      961 gggggaacag gttgatgggt cgggcgggct gcttgtccag tttgcggtgg agccggccgc
     1021 gcgcccacca cgcaacggga ctgcagccga ggacgatcgt gcccgtggcg tggtagccca
     1081 gcgccacgat cgcgatcgag atccacggcc cgacggcggt cagccacgag agcgcgcccg
     1141 agccgaggag cacgatgacg agatcgagaa cggccgtctg gaaatagatt gtcgacagct
     1201 gccgccacgt cgggcggggc atcgtcaggc gaagcggtcc cgggacgagc gcgtcccggc
     1261 tcgtgtcgct gtcgcgatcc tcggggaaca agcggatgaa ctcgctcagc gttgactcgg
     1321 ccggcaggcc gacgtgcact gcgtactcac ggaagaacgc ccggcggtag atgccggtcg
     1381 gccagcggga caggtggccg cgctcgagct cggcgaagag ggactcgctg accttggtga
     1441 cctcggcgat cctcaagagg ctgatgccgc ggctctcgcg tgccgccctg agctcctcgc
     1501 agaacgcccg gcggagctcg atcgcggcgg cctcacgctc ggcgcgagtg ggcgcgctcg
     1561 tcagcgttcg tggcgccgat gccagaccat cgtcgtggtg gcgggcgagc gcggtttcgg
     1621 aaacggcagg catccctggg gatcgagtta ggaaggggcg atcttactcc gactcgcacc
     1681 gccggtgcgt gcgtttttga cgactgccgg ccggcctacg gcaccgccca gtattcgggc
     1741 aggtccgctt tcgtctctct caggatatcg atgatggccg tgcgatcggc gcggctgagc
     1801 gtcgagtatc ggtcgcccgt ctcccgcccg ctgaggacgt cccacattct ccggtagacc
     1861 gcggccttgg cggacggggg cagtccgtcg aagggctctg agtagaccaa gtagctgcag
     1921 ggatacttga gcagccggcg ctcgagatcc aggtcccgta gcgaccgccc cttgctatcg
     1981 cggggccccc gcgcgccgaa ggccttcgcg aaagccgtgg gaccgctgat cggtccaggc
     2041 agggggttct cgtccacgaa gaggaggtag tccacgagct ccgcggccgc cgcttcgagc
     2101 ggcctccgag cctcggcacc gaccctggct tcccacccta cgcgggtgat gaggttcagc
     2161 atctgcacct ggtgctccag taccagcagg gccacgatgt cgctgtgggg tgagaggtat
     2221 cccgtcatgt cgaagcggcc gtcgagccgg gcgacgtgca cgctttccgg cgtgatcatc
     2281 gctcccaggt cggtcggatc ggtcgcgacg gcgttcccca tgtgcctgga tgcctggtga
     2341 ctgccggtga cgaaccagcc cccccaacgc agttcgaatg gacttcgatg gtcggtcgta
     2401 aaggccggtc cgtacatcgt cgttccgtca acggaaggaa acacgctgcc gacgaacatg
     2461 ccgggaacgt tgtgcgtggc atcggaggtg tggcacatca cgcacgcgtc gtttcgctcg
     2521 aattgcggta cggccgtgtg agtctgctcc agcgtgtaga agatggcgcc ctgcttggga
     2581 tcgtgcgaca cgaactccag cacgggtccg gagcgcaccc acccgaccgc gaccgtgtcg
     2641 ttgaagaaga ttgcgcgcgg gttcgcgggg ccgatcttcg tcgcctggaa gctggttttc
     2701 gagaagacga ggacctgcga ctcaatcggc acgtcgaggg cgtcgagcac ggctttcaga
     2761 tacccggagg gcccgccccg gtcgagcgcg acctcgccgc gctgcagccg gccgttcaac
     2821 cgggcgacgg catcggtcac cggcgtcgtg gcgtaggcga ttgccgggtg attgcggaga
     2881 agagacgggt gcacgtcgcc ggaaagaacg acgaggctgc cggcccaggc gagaacgccg
     2941 gccaggattc gaatgttgaa catgtgattc ggcaccggaa cctgcaaagg gtgctcccga
     3001 tgcgcatcgc gggatttcgt tgaaaacgcc ttgatcgatg ggcgcagtgc gtaattgtcc
     3061 gcacccgttg ggcaaaaccc cgcggtcggc cggcgcctgc ccgcgacaac gtttacagaa
     3121 agagccgatg gcccccgctg tggaggtaat tcatcgcctg cacggtgagc aggacggtga
     3181 actcctgccg cgcgtgttcc tccggcaccg cgagccggag cttgagctcg cggcagcgcc
     3241 gcatcatgtc ctcgagcact ttgtcgatgg tgtactggta gatccccgtc catccggcaa
     3301 ccgccttgcg caccgtcggc ctgatacgcg aaatgaattg ggcggcggtt ggatggctcg
     3361 agaattccgg cgcgtcggag aacagccggc gcaggtcgcg gtcgtagaag ttcgggtagt
     3421 ccaccccgta gtgccgccgc ttgttcttgt agtgctgcct cagcgtcgtg cggatggcgg
     3481 tcaacgggtc cacctcgttg cggttctgaa acggcggtgg aacgtcgcga agcgagctca
     3541 tcaacgcgtc catgtattcc agcttggtga gcgccttcca gcccgtgtag cgggggcgcc
     3601 attcgctgtt cggcgtcagc cagagcgcga acgtttccgc gaagtcctcg tcagggtggc
     3661 tctgcgcgta ccacgcgtcc agatggagca cgaaactctt gctgtacggc ttcggcgtgt
     3721 agaactcggg atacggcttg cgaggcgacc cgaagatctg cctgcgcttc tgccgtcttc
     3781 ggaggcggta cgcgttgtcg atcgcgtgcc ccgcctcgtg gcggaggatg cgcatgcacc
     3841 actcgtgctc gccgccttcg acttcgagca tctgcttcct ctcgaggttc tcgagccgcg
     3901 ggtgcgcgag gtagaacggg atcgcgactg ccgtcgatcc gtccggcgtg aaccactcgt
     3961 ccgagaggta gaagtgcagc ggaaagccga gcccgcgcgc gcgcagctct tcccggatct
     4021 gttcgatccg cgcggccagc gttccctcga tctcgagcgg caaatccgac aggcggacgt
     4081 ccagcagcgc ctcgtcgtcg aggtccggcc aggtgtccgg cagggggcgc ttgcgcacgg
     4141 ctggatatag actagctcgt agacggtagc tggtagccag ggagtccaga tgcgagccgt
     4201 gtgggcgggt cttgcggtga taggtatgac ggtgctgatg acagggcagg aacgcgatcg
     4261 ggcgaagatc gcgcaggact acaagtggaa cgtcgccgag gtctatcccg acgatgccgc
     4321 ctggcgtgcg cgcaaggagg ccctcacgaa ggagctgtcg tcggtgcgcg gtttccaggg
     4381 gaagctgggc tcctcaccgg ccacgatggc cgaggcgctc gagacgatgt cgcgcctcga
     4441 caaggagctg gcccgcttgt acgtctacgc gagcatgcgg tccgacgagg acacgcgcgt
     4501 gtcactcacg caaggcatgc agcaggagat gcagcagatc gccgcgaagt tcggcgcgga
     4561 ggcctccttc atcgagcccg aggtcctgaa ggtcggcagc gcgtcgatcg aggaggcgat
     4621 tgcctcggaa cggcgcctcg cgccgtacgc gttctacctg cgcgacatcg cgcgacgcga
     4681 gccgcatacg ctgtcggacc ccgaggagaa gctgctggcc gacgtggcgc cgctcgccgg
     4741 atcgcccacc aacatcttca cgattctgtc caacgcggac tttccctacc ccacgatcac
     4801 gctcgccgac ggacgccagg ccaaggtcga tcaggccggg tacaccgagc ttcgcaccgc
     4861 gccgagccgg gccgatcgtc aggcggcgat gtcggccttc ttcggcgcgc ttggcgcgtt
     4921 cagccgcacg ctgggcgtca ccatgaactc gagcgtgcag aagacgctgt tctactcgcg
     4981 ggcgcgaaag cattcctcca atctcgaggc gtcgctcaac gggccgaacg tccccgtgtc
     5041 ggtgtacacg cgcctggtcg agggagtgaa ccgtcacctg ccgacgttcc accgctacct
     5101 tcggctccgg aagcgaatga tggggctgac cgacgatctg cactactacg acctgtatgc
     5161 gccgctggtc gcgtccgtcg atctgcgcta cacgcccgag gaggcgcagc agcacgtgat
     5221 cggcgccatg gcgccgctcg gaaccgacta cgtcggagtc ctgcggcgcg cgttccgcga
     5281 gcgctggatc gactggtacg cgaccgaggg caaggtgtcg ggcgcctact cgaatggagc
     5341 ggtctacgac gtccacccgt acatgctgct caactacctc gggcagtaca acgacgtgag
     5401 cacgctggcc cacgagctcg ggcacacgat gcagagctac tactcgaaca aggtccagcc
     5461 gtatccgacg gcgagttacc cgacgtttgt cgccgaggtg gcgtccacct tcaacgaggc
     5521 gctgctcatc gactacatgc tgaagcagat caaggacacg cccacgcggc tctcgctcct
     5581 cggaaactac ctggagggca tcaaggcgac tctcttccgc cagacgcagt ttgccgagtt
     5641 cgagctgcgg atgcacgaga tggggcagaa gggcgaaccg atcaccggcg aaggcctcgc
     5701 caggctgtac ctggacatca cccgcaagta ctacgggcac gaccagaagg tcaccgtcgt
     5761 cgacgactac atcgcccacg agtggagcta catcccgcac ttctaccgtg acttctacgt
     5821 gttccagtac gcgacgtcgt tcaccgccgc cgaggcgctg tcggcgaaag tgctggcagg
     5881 cgatcagtcg gcgacgcagc ggtacttgac gttcttgtcg gccggcggat cgaagtaccc
     5941 gatcgatctc ctgaaagacg ccggcgtgga catgacgacc gacgagcccc tcgatctggc
     6001 cgtcaaacgg atgaacgtcg tgatggacga gatggagaag ctcatcgctt cgcgataacg
     6061 tccaactccc gattcccaac tcccaatgaa tgtcttgggg gttggtggtt gggagttaca
     6121 ggattccttt tcccagtgcg gacagcgcca gctccgcgcc gaactcggcc gtcgtattcc
     6181 ggatgtcgag cgtcgggttg acctccacga gatcgagcgc caccaggcgc tgggaatcgg
     6241 ccaccagctc catcaccatg tgcgcctcgc ggtagtcgag cccgccacgt accggcgtgc
     6301 cgacgcccgg ggccaccgcg gggtcgcatg catccaggtc gaacgacacg tggatgccgc
     6361 cggtgcccgc cgaggcgagc gcgatcgcct tttcggcgac gacggcaatg ccatgccgat
     6421 cgatgtcctt catcgtgaac acgtgcacgc ccgattcgcg tatccggtcc ttttcgcgct
     6481 cgtcgaggtt gcggatgccg acgagcaccg tgcgatcggc gagcaccgac ggcgaggcgc
     6541 cgatcgacgc gagttcctcc ggcccctggc cgagcagcgt cgcgagcggc ataccgtgga
     6601 cgttgccgct cgtcgtggtc tcgggagtgt tcatgtcgcc gtgcgcgtcg atccagatga
     6661 gacccatcgg gtacgacgtc atgcgacgga cccagtcggc gcttgccgcc accgatccgg
     6721 ccgccagact gtgatcgccg ccgaggacca gcggcacggc gcctgcgtcg agcgcgccgg
     6781 cgacggcgga gtaaacgccg gcgcacactc ccgcgatctc gcggacgtat ttcttccgcg
     6841 ggtccgtttc ggggcgtgtt tcggcaatcg gcgccggcac gtctccaagg tcggacaccg
     6901 tcctgccgag cgccgcgatc tgcgggccga gcccggcaat cctgaaggca gagggaccca
     6961 tatcgacgcc ccggcgcccg gcgccaaggt cgagcgggac gccgatgatg tgaacgggct
     7021 tcacgtcaac tcccaattcc caactcccaa caggagttgc catgttatgc cacgacgggg
     7081 gatggaggcc tttgggagtt gggcgttggg cgtcgggagt tagagcgaag cgagatactc
     7141 caccgctcgg acggccgtgg cgcgtccgtc gctgatgcag tccgggatcc caatcgccct
     7201 gaacccgctt cccgtgacga agacgcctgg cagccctcca aggcgtcgct cgatcgcggc
     7261 gacccgttga aggtgaccgg tctcgtattg cgggctctgc ctggtccatc tgaacacacg
     7321 cgtcaggagc gcggagcccg aaatgccgag cagttcgtgc atctcgcggc gggccgtctc
     7381 gaccagctcc tcgtccgatc cgtcgaggcg cccgggatcg cggccgccgc cgaggaagcc
     7441 gcgaatcagg acgtggccgt ccgg
//
LOCUS       DBNGSH010000453         1974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3099178, whole genome shotgun sequence.
ACCESSION   DBNGSH010000453 DBNGSH010000000
VERSION     DBNGSH010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3099178"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="AC16CY_10535"
     CDS             complement(<1..340)
                     /locus_tag="AC16CY_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3354269.1"
                     /translation="MRRLLACGALALAASVAPSWTVSGQRADAGDVTYTRDVAPILYQ
                     NCAYCHRPGEVAPFSLLSYRDARPWAKAIERQVVERRMPPWNADPHYGEFRNPRRLSD
                     RDIATIVAWVE"
     gene            complement(415..783)
                     /locus_tag="AC16CY_10540"
     CDS             complement(415..783)
                     /locus_tag="AC16CY_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3354270.1"
                     /translation="MGESKLRVEEREAGDVTILVLSGEMLLDDGDLVFRKVIHDKVEK
                     GRVKILVDLAGVTYIDSSGVGMMAAKLKTVRDRGGDIRLMHLTSRGQRLLSLLKLRST
                     FETFEDETLALRSFERRPHG"
     gene            complement(794..1765)
                     /locus_tag="AC16CY_10545"
     CDS             complement(794..1765)
                     /locus_tag="AC16CY_10545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3354271.1"
                     /translation="MSKRIPEWTVATEDRNIRLDKFLAAADRLGSRSRVGQALERGRV
                     FLNETERAPKDAALRLRPGDVVRVWLDRPGSAKRRTGVFRTGDLQILYEDDVIVVINK
                     PAGLLTVPLPVRDAATSVYDQLTAHLGGGSRRRPYIVHRIDRDTSGLVLFAKTARGQR
                     ALKDQFMRREPQRVYAALVHGHPDPPAGTWRDVLAWDRKALVQKKARQADPRGREAVC
                     DYRVLEAFADSALLEVRLHTGKQNQIRIQARLHGHVLVGERKYVRHTDAGPSIEFSRQ
                     ALHAQRLSFRHPADGRPVSFEAPLPDDFSRLLRLLRSKPGTPRDGQI"
BASE COUNT          315 a          664 c          663 g          332 t
ORIGIN      
        1 cttcgaccca ggcaacgatc gtggcgatgt cgcggtcgct caaccggcgc gggttgcgga
       61 actcgccgta gtgcggatcc gcgttccacg gcggcatccg ccgctcgacg acctgtcgct
      121 cgatggcctt ggcccacggc cgggcatctc gataggacag cagcgagaac ggggcgacct
      181 cgccgggtcg atggcagtat gcgcagttct gatagaggat cggggcgacg tcgcgcgtgt
      241 aggtcacgtc gcccgcgtcg gcgcgctgcc cggagacggt ccaggacgga gccacggatg
      301 ccgccagcgc gagcgctccg caggcgagca gtcttcgcat acgcccctcc tcgtgggggc
      361 aattctagcc tgaatcgcag cccgcgattt tcggcctttc aagacgtggg cgactcaccc
      421 gtggggacgg cgttcgaagc tgcgcagcgc cagcgtctcg tcctcgaagg tctcgaacgt
      481 cgagcggagc ttcagcaggg acagcaggcg ctgacccctc gaggtgaggt gcatgagccg
      541 aatgtctccc cctcggtccc tgaccgtctt gagcttggcg gccatcatgc ccaccccgga
      601 gctgtcgatg taggtcacac ccgcgagatc gaccaggatc ttgacccggc ccttctcgac
      661 cttatcgtga atcaccttcc ggaaaaccag atcgccgtcg tcgagcagca tctcgcccga
      721 gaggacgagg atggtgacgt ccccggcctc ccgctcctcg acccgcagct tggattcgcc
      781 catgcgggcc gcattatatc tgcccgtcgc gcggcgttcc cggcttcgag cgcaagaggc
      841 gcagcaggcg cgagaaatcg tctgggagcg gcgcctcgaa gctgaccggc cgcccgtcgg
      901 cgggatggcg gaaggacaag cgctgcgcgt gaagggcctg gcgcgaaaac tcgatcgacg
      961 ggccggcgtc ggtgtgacga acatatttgc gctcgccgac gagcacgtgg ccgtgcaacc
     1021 gcgcctgaat gcgaatttga ttctgtttcc ctgtgtgaag acgcacttcg aggagggcgc
     1081 tgtcggcaaa cgcttccagc acacggtagt cgcagaccgc ttcgcgtccg cgtggatcgg
     1141 cctggcgggc cttcttctga acgagcgcct tccggtccca cgcgagcacg tcccgccacg
     1201 tgccggccgg cggatctgga tggccgtgca ccagggcggc atacactcgc tgcggctcgc
     1261 ggcgcatgaa ctgatccttc agcgcccgct ggccgcgcgc cgtcttcgcg aacagcacga
     1321 gaccggacgt gtctcggtcg atgcggtgga cgatgtacgg cctgcgccgg ctgccgccgc
     1381 cgaggtgagc cgtgagctgg tcgtacacag acgtggccgc gtcccgaacc ggaaggggga
     1441 cggtcagcag tcccgccggc ttgttgatca caacgatgac gtcgtcttcg tagaggatct
     1501 gcagatcgcc cgtccggaag acgccagtcc gccgctttgc gctgccaggc cggtcgagcc
     1561 agacgcgcac gacgtcgccc ggccggaggc gcagcgccgc gtccttcggc gccctctcag
     1621 tctcgttgag gaacactctg ccgcgctcga gtgcctggcc gacgcggctg cgcgacccca
     1681 gccgatcggc ggcggcgagg aatttgtcga gccggatgtt ccggtcctct gtggcaaccg
     1741 tccattcggg aattcgcttg gacacactaa gcggccccgg cctcgcaggg ttcgcccgcg
     1801 cagaggatac cggaaggcga ggcatggccc ttgcaccacg cctgtctatg aagacgccaa
     1861 ctctgctgca caacggtgag ttcgaggtac tcaaggcagg agtccacagt gtggtgctgg
     1921 gcctggcggc cctctgtgcc gcctacaact ttgccgcatg gctggtgcgc cggc
//
LOCUS       DBNGSH010000454         8247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4627285, whole genome shotgun sequence.
ACCESSION   DBNGSH010000454 DBNGSH010000000
VERSION     DBNGSH010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4627285"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="AC16CY_10550"
     CDS             complement(<1..215)
                     /locus_tag="AC16CY_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR3354272.1"
                     /translation="MTRVADFDFELPPELIAQRPPDERGRSRLLVLPRREGPVEHTTF
                     ARFPDYVRPGDLLVLNDTRVFPARLLGR"
     gene            complement(212..1153)
                     /gene="ligD"
                     /locus_tag="AC16CY_10555"
     CDS             complement(212..1153)
                     /gene="ligD"
                     /locus_tag="AC16CY_10555"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HIR3354273.1"
                     /translation="MTPDPITIPSHINDVTLKVDGRDVRLTNLRKPFWPELGITKGGL
                     IQYYADVAPVLLPHIRDRAMVMRRYPDGAAGASFFMKEAPSPRPPWIRICPIDHGDGK
                     VVNFPVIDDLPSLLWLVNLGCIDLNQWYARCDDVHRPDYLHFDLDPGEGATFDQVLDC
                     GLIVRDALTTLGMTPFVKTSGSRGLHVYVALVRGPLQEVVLTFAKALATELAARNPKL
                     MTVGYRVSARPRGRVLVDYKQNAWGQTLASIYSVRPRPTATVSTPLTWDEVARGARLE
                     DFRFDNVRARIAKAGDLWKPILAKKGRTNLDRFFGRT"
     gene            complement(1195..2211)
                     /locus_tag="AC16CY_10560"
     CDS             complement(1195..2211)
                     /locus_tag="AC16CY_10560"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR3354274.1"
                     /translation="MVSASGLPCRARISSLATCVPPRVLTNADLEKMVDTSDEWILQR
                     TGIRERHIVDSGVATSDLAAGAARTAIARAGLTPEDIDFIVVGTTTPDMLFPSTACLV
                     QHKIGATRAWGFDLFAACSGFTYALTTAAQMVSNGSSRHALVIGADVMSSIIDYTDRT
                     TCVIFGDGAGAAVVEASGDPDIGILDFENYVDGSGGDALCMPAGGSLRPASHDTVQQR
                     LHFVKQDGQAVFKFAVRNTEEACRRILQRNNLSADSIDLFVSHQANRRIILSAAEKLG
                     LPEDKVVINIERFGNTTAATIPLALSDAVDAKRLRKGDLLLMVSVGAGFTVGALLLKW
                     GLKE"
     gene            complement(2205..3221)
                     /gene="ruvB"
                     /locus_tag="AC16CY_10565"
     CDS             complement(2205..3221)
                     /gene="ruvB"
                     /locus_tag="AC16CY_10565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR3354275.1"
                     /translation="MDERLITPGRMDEDTQYEVGLRPRTLAEYIGQDRLRDNLHVAIA
                     AARGRREALDHVLLYGPPGLGKTTLAYVIGNEMGAPVRATAGPVIEKPGDLAGMLTSL
                     QEHEVLFIDEIHRMAPAIEEILYPAMEDYELDIVIGQGPGARSVKVTLQRFTLIGATT
                     RAGLLTSPLRARFGIVHRLDFYESQHIEEIVRRSARILDVAIDGEATAEIARRSRGTP
                     RIANRLLRRVRDYAQVRADGRITSEVAQAALTMLEVDDNGFDEVDRKLLRTIIDKFSG
                     GPVGVNSLAAAINEEKDAIEDIYEPFLIQAGFLDRTPRGRVATSRAYEYFGLTAPGRD
                     TRLW"
     gene            complement(3298..3900)
                     /gene="ruvA"
                     /locus_tag="AC16CY_10570"
     CDS             complement(3298..3900)
                     /gene="ruvA"
                     /locus_tag="AC16CY_10570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR3354276.1"
                     /translation="MIALLRGRVLDKQPNRVIVDVGGVGYDVHVPLSTFYDVGDEGAD
                     VTLRVHLHVREDALQLYGFLTELERQLFERLIGISGIGPKLAISVLSGMEPRDLIAAV
                     QRGDVARLTGIPGIGKKTAERIVLELRDRLARLVPPAAGGQAAEAPTGDRLRDDLVSA
                     LQNLGYHRPQADKAIQAVLAASPESTFEQALRGALRDLMK"
     gene            complement(3962..4516)
                     /gene="ruvC"
                     /locus_tag="AC16CY_10575"
     CDS             complement(3962..4516)
                     /gene="ruvC"
                     /locus_tag="AC16CY_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR3354277.1"
                     /translation="MFGIDPGSERTGYGCVETDGRRHRLVICGAIDAAGESFPRRLAN
                     IHRELSALLSRCRPECVVVENLFYANNARSALKLGHARGVAILAAVEAGCEVVEYSPA
                     EVKRAIVGYGRADKRQMQQMVKILLGLDKPPSPHDAADALAVAICHVHSGFGLRASGL
                     GPRDSSRIAQTPKTLKSWRHYRPA"
     gene            complement(4519..5268)
                     /locus_tag="AC16CY_10580"
     CDS             complement(4519..5268)
                     /locus_tag="AC16CY_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3354278.1"
                     /translation="MSGHSKWHTIKHKKGAADAKRGKIFTRLIKELTVAARNGGGDPG
                     TNPRLRTIIAEAKANNMPRENIERAIRRGTGEEPGVSYEEITYEGYGPGGVALLIQSL
                     TDNKNRTVGEIRHLLSKYNANLAAENSVAWMFTRKGQIVIEKGKADEDQILNVALDAG
                     ADDMNDDGGAWEIVSAPESFESVRDAVKGLGIEPASAEVAMIPQNYVKLQGKDAQVML
                     KLMEALDDHDDVQHVWANFDIEEKEIEASLA"
     gene            complement(5324..6112)
                     /locus_tag="AC16CY_10585"
     CDS             complement(5324..6112)
                     /locus_tag="AC16CY_10585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3354279.1"
                     /translation="MPQGHEGWDDYAPFYDWENARTFGRRDVSFWRNLALRCGGPVLE
                     LGSGTGRISLPLGRAGVPLVGIDRSLPMLLRARARVRRARLAGRVRLVRGDIRHLPFA
                     ASSTGGHGFSMVIAPYGLLQSLLRERDLAATLAEVRRVLRPGGSFGMELVADLPSWKE
                     YQKKTSLRGWRSRRGGVHVTLVESVRQDRPRRLTIFDQQFTERRGRSRRVHRFSLAFR
                     TLSVPHMARRLEKAGFDVDALLGDYHGAPWDARADVWVILAKAR"
     gene            complement(6160..7002)
                     /locus_tag="AC16CY_10590"
     CDS             complement(6160..7002)
                     /locus_tag="AC16CY_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3354280.1"
                     /translation="MTAAIDALISPTLKHLREAWWDDEFTEFLVETLKPRPGNRILGV
                     GCGEGLAEVAIGRRHISQVRLVGVDLAPSKVAAARQETASHNQRAQFAAGDASRLPFR
                     DAVFDSLYSVAVLQHVKDVQAAVIEFARVTAPRGRVVAVEPDNSARYGFSSTPAGRRT
                     FELSVPFFNALSGLAPDGRAPAVGPNLPRLFSEHGIEPLDVRLFPVSKTTLGAPDAAF
                     WEGRRNAVRRAFADGASEAARWLGREYLEALDAYEAEARQAGAAFVEIQNTMLFATVG
                     QKLE"
     gene            complement(7110..8225)
                     /locus_tag="AC16CY_10595"
     CDS             complement(7110..8225)
                     /locus_tag="AC16CY_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018764041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354281.1"
                     /translation="MSRELSFVTRCVGTVVCALIAATSAAAQGKAPTPQLVVVAATVS
                     HQSGLAASSAGDVLVLAGQNFGSGPTVYVAGVPVPVTSVSPDGTLLTAQLDGPLDPGS
                     YLVQVSRGPAETQNATFIVVAGVGGSGGGGTGPKGDKGDPGPAGPQGPTGPAGATGPA
                     GPTGPIGPTGATGPIGPIGPTGPTGATGPIGPIGPTGPTGATGPIGPAGPTGPIGPIG
                     PTGAIGPAGPTGPAGPAGVDMFTKFGRGNDPSVALAFLADPVEVAIGGPTQKVLVTSS
                     KGLGTLAALSGIWAGATDLDLWICFQESAGALTKVGVGVLDLSAAMDTRQLYTLSATS
                     TGLAAGTYKFGLCGSTGDPNGHWNWNEFSYTTALVTQ"
BASE COUNT         1368 a         2761 c         2718 g         1400 t
ORIGIN      
        1 cggccaagga gtcttgccgg aaagacccgc gtgtcgttga gtaccagaag atcgccgggg
       61 cgcacgtaat ccgggaagcg tgcgaaggtg gtgtgctcga ccgggccttc gcggcgcggg
      121 agcaccagca ggcgcgagcg gccgcgctcg tccgggggcc gctgcgcgat gagctccggc
      181 gggagctcga aatcgaaatc ggcaacccgt gtcatgtccg gccaaagaaa cgatcgagat
      241 tggtgcgtcc cttcttcgcc aggatcggct tccagagatc gcccgccttc gcgatgcgcg
      301 cgcgcacgtt gtcgaacctg aagtcctcga ggcgggcgcc gcgtgcaacc tcgtcccagg
      361 tgagcggggt cgagacggtg gccgtgggcc gcgggcgcac ggagtagatg ctggccagcg
      421 tttgccccca ggcgttctgt ttgtaatcca ccagcacgcg acccctggga cgcgccgaga
      481 cgcggtaacc gaccgtcatg agcttcgggt tgcgcgcggc cagctcggtt gccagcgcct
      541 tggcaaacgt cagcacgacc tcctgcagcg gaccccgtac gagcgccacg tacacgtgca
      601 ggccgcggga cccgctcgtt ttcacgaacg gcgtcatgcc cagcgtcgtc agcgcgtcgc
      661 gcacgatgag gccgcagtcg agaacctggt cgaaggtggc gccctcgccg gggtccagat
      721 cgaagtgcag gtagtccggc cgatgcacgt cgtcgcagcg ggcgtaccac tggttcaggt
      781 cgatgcagcc cagattgacg agccagagca gggacggcag gtcgtcgatg acgggaaagt
      841 tgacgacctt tccgtccccg tggtcgatcg gacaaatacg gatccagggc ggccggggcg
      901 acggagcttc cttcatgaag aacgacgcgc cggctgcgcc gtcgggatac cggcgcatga
      961 ccatcgcgcg gtcgcggata tgcggcagca gaaccggcgc gacgtctgcg tagtactgaa
     1021 tcagcccgcc cttggtgatg ccgagctcgg gccagaacgg cttgcggagg ttggtcagcc
     1081 gcacgtcacg cccgtcgacc ttcagcgtga cgtcgttgat gtgcgacgga attgtaatgg
     1141 ggtcgggagt catttgttgg cgagtgtcct caacaaatga ctcccgaccc ccttttactc
     1201 cttcagtccc cacttgagca gcagagcacc cacggtgaac ccggcgccga ccgacaccat
     1261 cagcaggagg tcgcccttcc gcagccgctt ggcgtccacg gcatcgctca acgcgagcgg
     1321 aatcgtcgcc gccgtcgtgt tcccgaaccg ctcgatattg atcacgacct tgtcttccgg
     1381 caggcccagc ttctcggccg ccgacaaaat gatgcgccgg ttcgcctgat gggacacgaa
     1441 caggtcgatg ctgtccgcgc tgaggttgtt gcgctgcagg attctccggc acgcctcctc
     1501 ggtgttgcgc acggcgaact tgaagaccgc ctgcccgtcc tgtttcacga agtgcagccg
     1561 ctgctggaca gtgtcgtgcg aggccgggcg gaggcttcct ccagcaggca tgcacagcgc
     1621 gtcgccgccg ctcccgtcca cgtagttctc gaaatcaagg atgccgatgt ccggatcgcc
     1681 agacgcctcg acgaccgccg cgccggctcc gtcgccgaag atcacgcacg tggtgcggtc
     1741 ggtgtagtcg atgatgctcg acatcacatc ggccccgatc accagggcat gccggctgct
     1801 gccgttcgac accatctgcg ccgcggtcgt caacgcgtag gtgaagccgg agcaggcggc
     1861 gaacaggtcg aagccccagg cccgcgtcgc gccgatcttg tgctggacaa ggcaagccgt
     1921 gctcggaaac agcatgtccg gcgtcgtggt gccgacgacg atgaagtcga tgtcctcagg
     1981 cgtcaggccg gcgcgcgcga tcgccgtcct cgcggccccg gcggccagat ccgaggtcgc
     2041 gacgccggag tcgacgatgt gccgttcgcg gataccggta cgctgcagga tccattcgtc
     2101 gctcgtgtcg accatcttct cgaggtcggc gttggtcagc acgcgtggcg gcacacacgt
     2161 cgcaaggctg gaaatgcgag cacgacacgg aaggccagaa gcacttacca caggcgagta
     2221 tcccttccag gggcggtgag cccgaaatat tcgtaggcgc gcgacgtcgc gacgcggccc
     2281 cgcggcgttc gatcgagaaa gccggcctga atcaggaacg gctcgtagat gtcctcgatg
     2341 gcgtcctttt cttcgttgat ggcggccgca aggctgttga cgcccacggg accgccgctg
     2401 aacttgtcga tgatcgtgcg cagcagcttc cggtccactt cgtcgaagcc gttgtcgtcc
     2461 acctcgagca tcgtcagggc ggcctgcgcc acctcgctcg tgattcgacc gtcggcgcgc
     2521 acctgtgcgt agtcgcggac ccgacgcagc aggcggttgg cgatgcgcgg cgtgccgcgc
     2581 gagcgccgcg cgatttcagc cgtcgcctcg ccgtcgatgg ccacgtcgag gatccgcgcc
     2641 gagcggcgca cgatctcctc gatgtgctgg ctctcgtaga aatccagccg gtggacgatg
     2701 ccgaagcgcg cgcgcagcgg cgaggtcagc aatcccgctc tcgtggtggc cccgatgagc
     2761 gtgaaacgct gcagcgtcac cttcaccgat cgcgcgccgg gaccctgccc gatgacaatg
     2821 tccagctcgt agtcctccat cgcgggatag aggatctcct cgatcgccgg cgccatgcgg
     2881 tggatctcgt cgatgaagag gacttcgtgt tcctgcaggc tcgtcaacat gccggcgagg
     2941 tcacccggct tttcgatgac cggcccggcg gtggcacgaa cgggcgcgcc catctcgttc
     3001 ccgatcacgt aggcgagcgt cgtcttgcca agcccaggcg gaccgtagag cagcacgtga
     3061 tcgagcgctt cgcggcgtcc ccgcgccgcg gcaatcgcga cgtgcaggtt gtctcgaagt
     3121 cgatcctggc cgatgtactc ggccagggtc ctcgggcgca ggccaacctc gtactgggtg
     3181 tcctcgtcca tccgacccgg agtgatcagt cgctcgtcca ctgtgtcaca tgctcgtccg
     3241 gctgaccacc ttcgccaagg ctacggcggt caaaaagccg gacactacgt acgtctgcta
     3301 cttcatcaaa tcccgcaacg cgccacgcag ggcttgctcg aacgtggatt ccgggctcgc
     3361 ggccagcacg gcctggatgg ctttgtcggc ctgcggtcga tgatagccga ggttctgaag
     3421 agccgagacg aggtcgtcgc gaagacggtc acccgtcggc gcctctgccg cctggcctcc
     3481 tgcggcaggc ggtacgagcc gggcgaggcg gtccctcagc tcgagcacga tgcgctcggc
     3541 tgtcttcttc ccgatgccgg gaattccggt caggcgtgcg acgtcgcctc gctgcacggc
     3601 cgcgatcagg tcgcgcggct ccatgcccga aagcaccgag atcgccagct tggggccgat
     3661 tccgctgatg ccgatcagtc gctcgaagag ctggcgttcg agctcggtga ggaacccgta
     3721 cagctgcagt gcgtcctcgc gcacgtgcag gtgaacgcgc agcgtgacgt cggcgccttc
     3781 atcgcccacg tcgtagaagg tggacagcgg cacatggacg tcatagccga cgccgccaac
     3841 gtccacaatc acccggttcg gctgcttgtc cagcacccgt ccgcgcagca gggcaatcat
     3901 ctcgcgggct tcgggctacc ggatacgggc tccggggctt cgccacacat tgccggatgt
     3961 actacgcagg tcggtagtgg cgccacgact tgagcgtctt cggagtctga gcgatgcgag
     4021 aggaatctcg cggcccgagg ccggaagccc gcagcccgaa gcccgaatgc acgtggcaaa
     4081 tggcgacggc gagcgcgtcg gcggcatcgt gcggggaagg cggcttgtcg aggccgagca
     4141 ggatcttgac catctgttgc atctgtcgct tgtctgcccg tccgtatccg acgatcgcac
     4201 gcttcacctc ggcgggggag tactccacca cctcgcagcc ggcctccacg gccgcaagaa
     4261 tcgccacgcc acgggcatga ccgagcttga gggcgctccg cgcgttgttc gcgtagaaga
     4321 ggttctcgac gacgacgcac tccgggcggc accgcgaaag gagcgcggag agttctcggt
     4381 ggatgttggc gagccgccgg gggaaggatt cgccggcagc gtcgatggcg ccgcagatca
     4441 ccaaacgatg tcggcggccg tccgtctcca cgcagccgta gccggtgcgc tcggagccgg
     4501 ggtcgatgcc gaacaccctc acgccaggga ggcctcgatc tccttttcct cgatgtcgaa
     4561 gttcgcccag acgtgctgca cgtcgtcgtg gtcgtcgagg gcttccatca acttcagcat
     4621 gacctgcgcg tccttgccct gcagcttcac gtagttctgc ggaatcattg cgacttcggc
     4681 gctggccggc tcgataccga gccccttcac cgcgtcgcgg acgctttcga agctttcagg
     4741 agcggacacg atctcccagg cgccgccgtc gtcgttcatg tcgtcggcgc cggcatcgag
     4801 cgccacgttg aggatctgat cctcgtcggc cttgcccttc tcgatgacga tctgcccctt
     4861 cctggtaaac atccaggcca cgctgttttc cgccgcgagg ttggcgttgt acttcgacag
     4921 caggtgccgg atctcgccga ccgtccggtt cttgttgtcc gtcagcgact gaatcagcag
     4981 ggcgactccg ccgggtccgt acccttcgta ggtgatctcc tcgtaggaga cacccggctc
     5041 ttcacccgtg ccgcgccgaa tcgcgcgctc gatgttctcg cggggcatgt tgttggcctt
     5101 ggcttccgcg atgatcgtcc gcaggcgcgg gttcgtgccg gggtcgcccc cgccgttcct
     5161 ggcggcgacg gtcaactcct tgatgaggcg cgtgaagatc ttgccgcgct tggcgtcggc
     5221 ggcgcccttc ttgtgcttga tcgtgtgcca cttggaatgg cctgacatgg cggaaactcc
     5281 aggaaatccg gggaagctgg accggtcatt ctagcacgtc ggactaccgc gccttcgcaa
     5341 ggatgaccca gacatcggct cgcgcgtccc atggcgcacc gtggtagtcg cccagcagag
     5401 cgtccacgtc gaagccggcc ttttcgagac gccgggccat gtgcggcacg ctcagcgtac
     5461 gaaacgcaag ggagaagcga tgaacgcggc ggctcctccc tcggcgctcc gtgaactgct
     5521 ggtcgaagat cgtgagccgg cgaggccggt cctgccgcac cgactccacc agggtcacgt
     5581 ggacgccgcc gcgtcgactc cgccagcctc tcaggctcgt cttcttctga tactccttcc
     5641 acgacggcag atcggcgacc agctccatgc cgaacgatcc gcccggccgc agtactcgcc
     5701 ggacctcggc aagcgtcgcg gcaaggtccc gttcgcgcag cagtgactgc agaagaccgt
     5761 atggcgcgat caccatcgag aagccgtgtc ctccggtcga ggacgccgcg aacggaaggt
     5821 gcctgatgtc gcctcgaacc agtcgcaccc ggccggccag ccgggctcgg cgcacgcgcg
     5881 cgcgagcacg cagaagcatg gggagggaac ggtcgatgcc caccagagga acccctgcgc
     5941 gcccgagggg gagcgagatg cgacccgtgc cgctgccaag ctccaggaca gggccgccgc
     6001 aacgaagcgc gaggttgcgc cagaaggaca cgtcgcgccg tccgaacgtc cgtgcgttct
     6061 cccagtcgta gaacggtgcg taatcgtccc agccttcatg accttgcggc atgtgatcca
     6121 gttttgagtt ttgagtcttg agttttgagt tacagagttc tactcgagct tctgtcccac
     6181 cgtggcaaag agcatcgtgt tctggatctc gacgaaggcc gctcctgcct gtcgcgcttc
     6241 agcctcgtat gcgtcgagcg cctcgaggta ctcgcgtccg agccatcggg ccgcctcgga
     6301 cgctccgtcc gcgaacgcgc gccggacggc gttccgccgg ccctcccaga acgccgcgtc
     6361 gggggcgccg agcgtcgtct tcgacaccgg gaacagccgg acgtcgagcg gctcgattcc
     6421 gtgctccgag aacagacgcg gcagattcgg gccgacggca ggcgcacggc cgtcaggtgc
     6481 aaggccggac agcgcattga agaagggaac cgacagctcg aacgtgcgcc ggccggcggg
     6541 agtcgagctg aacccgtatc gtgcgctgtt atccggctcg acggcgacga cacggccgcg
     6601 cggcgccgtg acacgggcga actcgattac ggcggcctgg acgtccttga cgtgctgaag
     6661 gacggcgacc gagtagaggg aatcgaagac ggcgtcccga aacggcagcc gggaggcgtc
     6721 gccggcggca aactgcgcgc gctggttgtg tgatgccgtc tcctggcgcg ctgccgcgac
     6781 cttcgagggc gcgagatcta caccaacgag ccggacctgc gagatatgac gacggccgat
     6841 cgcgacctcg gcgagcccct cgccgcagcc gacgccgaga atgcggttgc ctggtctcgg
     6901 cttcaacgtc tcgacgagaa actccgtgaa ctcatcgtcc caccacgctt cacggaggtg
     6961 cttgagcgtg ggcgagatga gagcgtcgat cgccgctgtc acggggaaga attataggcc
     7021 ccagggccca ggccccagga atcagggctg ggccaggcct gaggccttgg acagctctga
     7081 ggcctgaggc ctgggccctg gggcctgctt tactgagtga cgagcgccgt cgtgtagctg
     7141 aactcgttcc agttccagtg cccgttcggg tcgcccgtcg agccacagag cccgaacttg
     7201 tacgtgccag ccgcgagccc ggttgacgtc gcgctcagcg tgtagagctg gcgtgtgtcc
     7261 atcgccgcgc tcagatccag cacgccgaca cctacctttg tgagagcgcc cgcggactcc
     7321 tggaaacaga tccacagatc gagatcggtc gcgccagccc atatcccgct gagggctgcc
     7381 aaggtcccga gccccttgct ggatgtgacc agcaccttct gagtcgggcc gccaatggcg
     7441 acttcaacag gatccgctag aaatgccaga gcaacgcttg gatcgttgcc gcgaccgaac
     7501 ttcgtgaaca tgtcgacacc cgccggcccg gcgggtcccg tgggcccggc aggtccgatt
     7561 gccccggtcg gtccaatcgg cccgatcggt ccagtgggtc cagccggtcc aatcgggccg
     7621 gttgcgcccg tcggtcccgt gggtccaatc ggtccaatcg ggccagtcgc gcccgttggt
     7681 cccgtgggtc caatcggtcc aatcggaccg gtcgcgccgg ttggccctat gggcccggtc
     7741 ggtccggccg gtcctgtcgc tcctgcgggg cctgtgggcc cctggggacc ggcagggcct
     7801 ggatcgccct tatcgccctt gggacccgta ccgccgccgc cactgccacc aacgccagcc
     7861 acgacgatga aggtggcatt ctgggtctcc gcagggccgc gggagacctg cacgaggtac
     7921 gaaccgggat cgagtggacc gtccagctgc gccgtcagaa gcgtgccgtc aggcgatacg
     7981 cttgtgaccg gcaccggcac tcccgcgaca tagaccgtgg gaccgctgcc gaaattctgg
     8041 ccagccagga ccaggacatc accggccgac gacgcggcca gcccggactg gtgcgaaaca
     8101 gtggcggcga ccaccacgag ctgcggagtg ggggccttcc cttgcgccgc tgccgacgtc
     8161 gcggcgatca gcgcgcacac gaccgttccg acgcagcgag tgacaaacga aagctcacga
     8221 gacatacgtc accttccttg gatccag
//
LOCUS       DBNGSH010000455        11162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3099183, whole genome shotgun sequence.
ACCESSION   DBNGSH010000455 DBNGSH010000000
VERSION     DBNGSH010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 11162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11162
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3099183"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="AC16CY_10600"
     CDS             complement(<1..866)
                     /locus_tag="AC16CY_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3354282.1"
                     /translation="MTLLGDARARLTALVSRCVAILAGHPHQAETARQLADFKRALDH
                     AAIVATTDVTGRITYVNDTFCRISEYSREELLGQDHRIVNSGFHDKEFIRDLWRTIAA
                     GHVWQGEIRNRAKSGRIYWVDTTIVPFLNERGKPYQYIAIRSDITARREAEEKLAQQA
                     TLARVGQLAAVVAHEVRNPLAGIKGAVQVLMSRRSAGDAELPVLRDIVGRIDSLSELI
                     NDLMLYARPRTPKLGATELRSLVSDAVSIVKRDPSAQSIEVAVDGGDVSASVDAEMVR
                     ATVLNLLLNAAQA"
     gene            953..2311
                     /locus_tag="AC16CY_10605"
     CDS             953..2311
                     /locus_tag="AC16CY_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3354283.1"
                     /translation="MSAATLLIVDDESLLRWSLRERLAREGYDILEAGTAAEALAAAS
                     NGIDLVLLDFKLPDGDGLSVLRQIKDQTPDALVILMTAFSTVENAVEAMKLGAYHYVN
                     KPFNLDEVALLVEKALETSQLRREVKALRASQGREYGFDAIIGSSPAMTAIKSLLVRV
                     AKSPASTVLLTGETGTGKDLAAKAIHYNSDRAGRAFLNITCSALPEQLLESELFGHER
                     GAFTDARQQKRGLFETADGGTIFLDEIGEMTPGLQAKLLRFLEEKSFKRVGGLADIRV
                     DVRVVAATNRDLEKEVKSGKFREDLFYRLQVMPIVLPPLRERHGDVVLLASYFIDRFN
                     REFRKKIRGLKPAATALLEQYQWPGNVRELRNAIERAMLLVDRDHLEPTDFNLTRTLT
                     PVEFALPAEGVSLDEVERQLLIQALERSRGNQTQAAQLLGINRDQVRYRIEKFGLAKA
                     NA"
     gene            complement(2313..2816)
                     /locus_tag="AC16CY_10610"
     CDS             complement(2313..2816)
                     /locus_tag="AC16CY_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3354284.1"
                     /translation="MRFTRILVPTDFSPASEAALAAAMELADRFGASIHLLHVLEDPY
                     STSAYATEVYGFLPPGLRETWQENAEKRLDALLPRERGTRSAGSCAVVFGSPARAIVD
                     HAHGAGIDLIVMGTHGRSGVAHLLLGSVAERVVRTATCPVMTVRGVTRPDAPAARETA
                     TPVGAGR"
     gene            3014..3349
                     /locus_tag="AC16CY_10615"
     CDS             3014..3349
                     /locus_tag="AC16CY_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3354285.1"
                     /translation="MNRIGVLALSFSLLVIPNLASAQSAAERGQKVYAAQKCNICHSV
                     AGVGNKRGALDEVGSKLSADDIRRWIVDATAMAAKAKAERKPPMKSYMLSKEDLDALV
                     AYMASLKKK"
     gene            3377..4714
                     /locus_tag="AC16CY_10620"
     CDS             3377..4714
                     /locus_tag="AC16CY_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HIR3354286.1"
                     /translation="MSTPVQLPDAVRNPVSLAGMALVTAAAVLFLVLFLLESFGFLVN
                     PYLGLLVFVTLPALFLLGLALIPFGARRAARRRRLHPETPEWPVFDLRQPRQRTILVA
                     VLALTFVNIVIVSLAAYGSVHYMESNAFCGQVCHETMAPEFEAHQAWPHASVECTACH
                     VGPGVDSFVESKLSGMRQLYHLATGQVPRPIPAPATSLGRTTDTCGGCHWPERSYGTV
                     VKESREYADDEPNSETVTRLLMNVGSPTVGGIHRHLAFDIEYVAADERRESIPLVRVR
                     NDRGEVKEFRADGATNDQIAGGTTRRMDCLDCHNRPAHTFSSSPQRAVDRALALNRVA
                     RQVPFVRREAAAALGGEYADRAAALDAIAKRLNEFYRSRPDVDARTVARVVTATQQVW
                     AQNVFPSMRVTWGTYPRHDGHVDTPGCFRCHDDRKAADGTTISQDCELCHSIQ"
     gene            4764..5735
                     /locus_tag="AC16CY_10625"
     CDS             4764..5735
                     /locus_tag="AC16CY_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HIR3354287.1"
                     /translation="MSRPRFFLFVAGSLFLLAVALNAAGVDRESPPAPRAAVAPLASA
                     QAKPQTPPAKYMGSDICLGCHSDMEKGLTGTMHGNAAHPRSPAAAQGCESCHGPGEFH
                     LEDPSVETSIRKFTRMAPREVSEACLSCHTKSSHQLWQGSAHDARNLACTTCHSVHTP
                     KAPRAQLKTATEAELCAGCHKAQAVKIRRVSHMPVFEGKLECSSCHNAHGATNVRLLR
                     VGNWINETCVSCHTEKRGPFLFEHAAGRESCVTCHDPHGSTNDRMLVARPPMLCQRCH
                     IGTKHPSTIYDNPDVVARSNRIIGRGCVNCHQQIHGSNHPSGSTFLR"
     gene            5745..7904
                     /locus_tag="AC16CY_10630"
     CDS             5745..7904
                     /locus_tag="AC16CY_10630"
                     /inference="COORDINATES: protein motif:HMM:NF023282.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HIR3354288.1"
                     /translation="MRFQHTLLLAAFISINGAPSAAQSSASTSTAQAQPAAGSATGVG
                     EIDVGARFTSGFDDPGRFNSFVDPSSGATLERLRYTRTRDAWIFDAAIDNAGYRDQRY
                     AASIERAGRLKASFEWNQVPLWYSAVGAGPYTVEGPGVLRLSDAGQSAVQGGATVSSA
                     FGSLVEPFDTRSRREIADARLVYSATRDLDLSVQFRSMARKGEMPYSASFGFSNAIEV
                     PATVDTRTNDLNVAAQWSNPRGAFRVAYDASMFNNSVDTLVWDNPLRITDRVDGGGAG
                     ASQGRLPLWPDSSAHTVSASGSLNLPARSKAVAYVSIGSWLQDAELLPHTINTAIQPI
                     PLARSTAEGDARIVSMMYRLSSRPTPTLWLNGQYRLYDYDNQMPVFAYSQYVRMDGAA
                     YSSPTGANDLFSMTRQFADVDASFTPFRFVAFRAGWGFENDDRSHRYTESTTENTARF
                     SIDSTSFSWGSVRLQYEFSTRTGEGLDEQVFSDLGEQASLRQFDISDRNRHRVAGILQ
                     VAVSNAVSVSASVSVGQEHRPETEMGLQDNDLTGFSVGVDFVPSDTVDFGVMYGLDNI
                     STLQKSRQADPGPQFNDPTRDWTTNMDEDVHTLSASLNLPRITDRAGFYAMYDYVYSN
                     TLYVYGLAPNSTLAPVEQLPALTSGFHRGSVGFDYRLTGRATLGVGYRLDSWEVSDFS
                     RSPATLNTPLINAFVNTLYQWAPYNVHTGFVRVRYRW"
     gene            7982..8626
                     /locus_tag="AC16CY_10635"
     CDS             7982..8626
                     /locus_tag="AC16CY_10635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3354289.1"
                     /translation="MPPIDPVQVLLAFLVLIVSLSVHEAAHAWSADRLGDNTARLLGR
                     VSVNPMVHIDVIGTVVFPLVSMVSGLPLIGWAKPVPVDLRNLGIDWRRKWMLVAAAGP
                     ASNLVIAAAAAIVFRLMPGGGLDGEAFASGSPVSRVLLRLVDLNVLLAVFNMIPVPPL
                     DGGNVLSGLLGGPAAAAFDRLRPYGFLILYGLLLTGLLWTLIDPLHTAALRWLL"
     gene            8653..9630
                     /gene="trpS"
                     /locus_tag="AC16CY_10640"
     CDS             8653..9630
                     /gene="trpS"
                     /locus_tag="AC16CY_10640"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR3354290.1"
                     /translation="MVSGMRPTGKLHLGHLVGALRNWAALQERYDCYYFVADWHALTS
                     EYADTGPLAANALDNVADWIAAGVDPERSTIFVQSLVPEHAELSLLLSMVVPIPWLER
                     VPTYKEQQEQLADKDLSTLGFLAYPLLQTADIAIYDGRFVPVGEDQVAHLELSREAIR
                     RFHQFYGEVLVEPQPLLTATPRLPGIDNRKMSKSYGNTIDLTDDDETVVKKVRQMYTD
                     PKRIRADIPGTVEGNPVFMYHDAFNDDAGEIEDLKARYRAGKVGDVEVKTKLARALNS
                     TLEPLRQRRREVNARPDRIREILVEGSRKARVVAQATMERVRDAVRLRY"
     gene            9630..10493
                     /locus_tag="AC16CY_10645"
     CDS             9630..10493
                     /locus_tag="AC16CY_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR3354291.1"
                     /translation="MKADGHEGLSAEALGAKVERDHAREHPDFESSPDAYRVKLEAFE
                     GPLDLLLHLIRTNEMNIYDIQISLITEQYLAYVELMQDLDLDVAGEFLVMAATLIHIK
                     SRTLLPRPDPAQDDEPEEDPREALVRRLLEHQKYKAAAGLLHERELLRSAQFMRPDGR
                     VAEIAGEEYEPELEVDLFSLLAAFKGVLERANRRGAMVLPAEQTSIEDRIKQLLARLS
                     ETEACGFEDLFNDGDGTRAFVIVTFLAMLEMIRLKLIRVFQSGGAGPIRVYKRARPVD
                     APHPIDDPLGP"
     gene            10501..11145
                     /gene="scpB"
                     /locus_tag="AC16CY_10650"
     CDS             10501..11145
                     /gene="scpB"
                     /locus_tag="AC16CY_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3354292.1"
                     /translation="MPNDVFESDPPASAVVSAEVKAVVEALIFASPEPITPKMLFKLL
                     AEEPKEDVLAAIDALRTDYENRPGLQMLEVAGGYQIVTRPDLNDWVRRLFHERSTQKL
                     TAQALESLAVIAYKQPVTALEIGEIRGVNTAGVLSTLLERHLIKIVGRKNVVGRPFLY
                     ATTREFLIRFGLKDLNDLPKVEDMAEALGFDPPAGLAETSPPEEMLPIEDDGES"
BASE COUNT         1959 a         3640 c         3714 g         1849 t
ORIGIN      
        1 gcctgcgccg cgttgagaag caggttgagc accgtcgcgc gcaccatttc cgcgtcgacc
       61 gacgccgaga cgtcaccgcc gtcgacggcg acctcgatcg actgggccga tggatcgcgc
      121 ttcacgatgc tgaccgcgtc gctcacgagc gatcgaagct ccgtggcgcc gagcttcggg
      181 gtccgcggtc tcgcatacag catcaggtcg ttgatcagct cgctcagcga gtcgatgcgc
      241 ccgacaatgt cgcgcaggac ggggagctcc gcgtctccgg cgctccgccg tgacatcagg
      301 acctgaacag cacccttgat cccggcgagg gggttgcgta cttcatgcgc cacgacggcc
      361 gccagctgtc caacccgtgc gagcgtagct tgttgcgcga gtttttcctc cgcctcccgg
      421 cgcgcagtga tgtccgatcg gatagcgatg tactggtaag gctttccgcg ctcgttgagg
      481 aacggaacga tggtcgtatc gacccagtag atgcggccgc tcttggcgcg attgcggatc
      541 tcgccctgcc agacgtgccc ggccgcgatc gtccgccaca agtcccggat gaattccttg
      601 tcgtgaaagc cggagtttac gatccgatgg tcctggccga gcagctcctc gcgcgagtat
      661 tccgagatgc ggcagaacgt atcgttgacg taggtgatgc gaccggtcac gtcggtcgtg
      721 gccacgatgg cggcatggtc gagggcgcgc ttgaaatcgg cgagctgccg ggcggtctcg
      781 gcctggtgag ggtgtccagc aaggatcgcg acacaccgtg acacgagtgc cgtgaggcgg
      841 gcacgggcgt cgcccagcag ggtcattcgt gcgagctccc cggagatcgt agtggcgccg
      901 gaggcccggc gcaatgggct caccgagccg cgcgggcagg tatacttgcc gtgtgtcagc
      961 cgccacgctc ctgatcgtgg acgacgaatc gctgctccgt tggtcactgc gcgagcgcct
     1021 ggcgcgtgag gggtacgaca tcctcgaggc cgggacagcc gccgaagccc tcgccgccgc
     1081 atcgaacggt atcgatctgg tgctgctgga cttcaagctc cccgacgggg acgggctgag
     1141 cgtcctgcgt caaatcaagg accagacgcc cgacgccctc gtcatcctga tgacggcgtt
     1201 ctccacggtc gagaacgccg tcgaggcgat gaagctcggc gcgtaccact acgtgaacaa
     1261 gcctttcaat ctcgacgagg tcgcgctgct ggtcgagaag gcgctcgaga cgagtcagct
     1321 ccgacgcgag gtgaaggcgc tccgggcgtc tcagggacgc gagtacgggt tcgacgccat
     1381 catcgggagc tcgcccgcga tgacagcgat caagagcctc ctggtgcggg tggcgaagag
     1441 ccccgcgtcg acggtgctcc tgactggaga gacgggaacc ggaaaggatc tcgccgccaa
     1501 ggccatccac tacaacagcg atcgggccgg ccgggcattt ctcaacatca cctgctcggc
     1561 gcttcccgag caactgctcg agagcgagtt gttcgggcac gagcggggcg cgttcaccga
     1621 cgcgagacag cagaagcgcg gcctcttcga gacggccgac ggcgggacga tcttcctcga
     1681 cgagatcggc gagatgacgc caggcctgca ggccaagttg ctgcggttcc tcgaggaaaa
     1741 gagcttcaag agagtaggcg gtctcgccga catccgcgtg gacgtccggg tcgtcgcggc
     1801 gaccaaccgc gacctggaga aggaggtcaa gtccggcaag ttccgtgagg acctgttcta
     1861 ccggctccag gtgatgccga tcgtgctgcc gccgctgcgc gaacggcacg gcgacgtcgt
     1921 gctgctcgcc agctacttca tcgaccggtt caaccgcgag ttcaggaaga agattcgcgg
     1981 actcaagcct gccgccaccg cgctgctcga acagtaccaa tggcccggca acgtccgcga
     2041 gcttcgaaac gccatcgagc gcgcgatgct gctcgtcgat cgcgatcacc tcgagccgac
     2101 cgacttcaac ctgacgcgta ccctgacccc cgtcgagttc gcgctgcccg ctgaaggcgt
     2161 gagcctggac gaggtcgagc gtcagctcct catccaggcc ctcgagcgct cacgggggaa
     2221 ccagacccag gcggcgcaat tgctcggtat caaccgcgat caggtcaggt accgcatcga
     2281 gaagttcggc ctcgcgaagg cgaacgccta gactagcggc ccgccccgac cggtgtggcc
     2341 gtctcgcgtg cggcgggagc gtcaggcctg gtgacgcccc gtaccgtcat caccgggcag
     2401 gtcgccgtgc gcaccacccg ttcggcgacg ctcccaagca gcagatgcgc gacgccgctg
     2461 cggccgtgcg tgcccatgac gatgagatcg atgccggcgc cgtgggcgtg atcgacgatc
     2521 gctcgggcgg gggatccgaa gaccaccgcg cagctgccgg cggatcgcgt cccgcgctct
     2581 cgcggcagga gcgcgtccag tcgcttttcc gcgttctcct gccacgtttc cctcaacccg
     2641 ggaggcagaa agccgtagac ctcggtggcg tacgcgctcg tcgagtacgg atcttcgagc
     2701 acgtggagca ggtgaatcga cgccccgaaa cgatcggcca gttccatcgc cgcggccagg
     2761 gcggcctccg aggccgggct gaagtcggtc ggcacgagga tgcgagtgaa gcgcatgacg
     2821 tcctccagtt ccacataaac gcgtcacaga aacgacgcgg tcatcaccga cagcgagagc
     2881 acgagtcgtg ccgcgcacga cgtgagtgtc tgccccggtt tcaagctcga ccgcaggggt
     2941 cgggtgcgaa ttaattcccg gttgcgtgag atcgttcgag gagtagactg atagccgttt
     3001 tggaggcaaa tggatgaatc ggatcggcgt gcttgccctt tccttttcac ttctcgtgat
     3061 ccccaatctc gcgtcggcgc agtcggccgc cgaacgcggc cagaaggtgt acgccgctca
     3121 gaagtgcaac atctgccact cggtggccgg tgtcggtaac aagcggggcg ccctcgacga
     3181 ggtcggctcg aagttgtctg cggacgatat ccggcggtgg attgtggacg cgacggccat
     3241 ggctgccaag gcaaaggccg agcggaagcc gccgatgaaa tcgtacatgc tctcgaaaga
     3301 ggacctcgac gccctggtgg cgtacatggc gagtctcaag aagaagtaac cgcaatcgcg
     3361 ccggtcgaaa gtcagcgtgt ccacgcccgt ccagcttccc gacgccgtca ggaacccggt
     3421 gtctctggcc ggcatggcgc tcgtcactgc ggcagcagtc ctgtttctgg tcctcttcct
     3481 gctcgaatcg ttcgggtttc tcgtcaaccc gtacctgggc ctgctcgtat tcgtcacgct
     3541 gcctgcgctc ttcctgcttg gcctggccct gattcccttc ggcgcgaggc gtgccgccag
     3601 acgccggagg cttcacccgg agacgccgga atggccggtc ttcgacctgc gccagcctcg
     3661 acagcggacc atcctcgtcg cagtcctcgc cctcacgttc gtcaacatcg tgatcgtgtc
     3721 gctcgccgcg tacgggagcg tgcactacat ggagagcaac gccttctgcg gccaggtctg
     3781 tcacgagacg atggcccctg aattcgaggc gcatcaggcg tggccgcacg cgagcgtcga
     3841 gtgcacggcg tgccacgtgg gacccggcgt cgattccttc gtcgagtcga agctcagcgg
     3901 aatgcgccag ctctatcacc tggcgaccgg gcaggtcccg aggccgattc cggcgcccgc
     3961 gacgtcgctc ggcaggacca ccgacacctg cggagggtgt cactggcctg agcgctccta
     4021 cggcacggtc gtcaaggaga gccgggagta tgccgacgac gagccgaaca gcgaaacggt
     4081 gacacggctt ctgatgaacg tcggaagccc gaccgtcggc ggcatccatc ggcatcttgc
     4141 tttcgacatc gagtacgtgg ccgctgacga gaggcgcgaa tcgatcccgc tggtccgtgt
     4201 ccggaacgac cggggagagg tcaaggagtt cagggccgac ggcgcgacca acgatcagat
     4261 agcgggcggg acaacgcggc ggatggactg cctcgattgc cacaaccgcc cggcgcacac
     4321 gttttcctcc tcgcctcagc gtgccgtgga ccgcgccctc gcgctcaacc gcgtggcgcg
     4381 gcaggtcccg ttcgtccgcc gcgaagcggc ggcggcgctc ggcggcgagt acgcggatcg
     4441 cgcggcggcg ctcgatgcca tcgcgaagcg cctgaacgag ttttaccgat cgcgtccgga
     4501 cgtcgatgca cggacggttg cgcgcgtcgt cacggcgacg cagcaggtgt gggctcagaa
     4561 cgtgtttccg tcgatgcgcg tgacctgggg gacctacccg cgccacgacg ggcacgtgga
     4621 cacgccggga tgtttccgtt gtcacgacga ccgcaaggcc gcggacggca cgacgatctc
     4681 gcaggactgt gagctgtgtc actcgattca gtaggcagta ggcggtggag accggagacc
     4741 gaagaccgaa gacggagctc ggaatgagtc gaccacgttt ctttctgttc gtcgcaggat
     4801 ccctctttct gttggcggtg gcgctcaacg ctgccggcgt cgacagagag tcaccaccgg
     4861 cgccgcgggc agccgtcgcg ccgctcgcga gcgcgcaggc aaagccgcag accccgccgg
     4921 cgaagtacat gggcagcgac atctgcctcg gctgtcactc ggacatggaa aaaggactga
     4981 ccggcacgat gcacggcaac gccgcgcacc cgcgctcgcc ggctgccgcc cagggctgcg
     5041 agagctgcca cggcccgggc gagttccacc tcgaggatcc atcggtcgag acctcgattc
     5101 ggaagttcac gcgaatggcg ccgcgcgagg tgagcgaggc gtgcctgtct tgtcatacga
     5161 agtcgtcgca ccagctctgg caagggagcg cccacgatgc gcggaacctg gcgtgcacga
     5221 cctgccacag cgttcacaca ccaaaggcgc ctcgggcgca gctgaagacg gcgacggaag
     5281 cggagttgtg cgccggctgc cacaaggcgc aggcagtgaa gatcaggcgc gtctcgcaca
     5341 tgccggtgtt cgaaggcaag ctggagtgct cgtcatgcca caacgcgcac ggggcgacca
     5401 acgtgaggct gctccgggtg gggaactgga tcaacgagac ctgcgtgtcc tgtcacactg
     5461 aaaagcgggg gccgttcctc ttcgagcacg ccgcggggcg tgagagctgc gtcacctgcc
     5521 acgacccgca cgggtccacc aacgatcgga tgctcgtcgc gcgtccgccg atgctctgcc
     5581 agcggtgtca catcggtacc aagcacccgt ccacgatcta cgacaaccct gacgtcgtgg
     5641 ccaggagcaa ccggatcatc ggccgcggct gcgtcaactg ccatcaacag atccacgggt
     5701 ccaaccaccc gtccggcagt acgttcctgc gataggagga tgtgatgcga ttccaacaca
     5761 cactcctgct cgcagcgttc atctcgatca atggtgcgcc ttcggcggcc cagtcgagcg
     5821 catccacgtc gaccgcgcaa gcgcagccgg cggcgggctc ggccaccggc gtgggcgaga
     5881 tcgacgtggg ggcgcgattc acgagcggct tcgacgaccc gggccggttc aacagcttcg
     5941 tcgatccgag cagcggagcc acgctggagc ggctgcggta cacgagaaca cgcgacgcgt
     6001 ggatcttcga cgcggccatc gacaacgccg ggtaccgcga ccagcgctac gcggcgtcga
     6061 tcgagcgcgc cggccgcctg aaggcgtcgt tcgagtggaa ccaggtgccg ctgtggtaca
     6121 gcgccgtcgg cgccggaccc tacacggtcg aagggcctgg cgtcctgagg ctgagcgacg
     6181 ccggccagtc cgcagtccag ggaggcgcca ccgtctcgag cgctttcggc agtctggtcg
     6241 aaccgttcga cacgcgatcg cgccgcgaga tagccgacgc gcgcctcgtg tattcggcga
     6301 cgcgcgacct ggatctgagc gtgcagtttc gaagcatggc ccggaaaggc gagatgccgt
     6361 acagcgccag cttcggtttc agcaacgcca tcgaggttcc ggccacggtt gacacgcgca
     6421 ccaacgacct caacgtggcc gcccaatggt cgaacccccg gggcgcgttc cgggtcgcgt
     6481 acgacgcgtc gatgttcaac aacagcgtcg acacgctcgt ctgggacaac ccgctgcgca
     6541 tcacggaccg ggttgacggg ggcggagccg gcgcgtcgca agggcgcctg ccgctctggc
     6601 ccgacagcag tgcgcacacc gtcagcgcgt cgggctcgct gaacctgcct gccaggagca
     6661 aggccgtggc gtatgtctcg atcgggagct ggctccagga cgccgagctc ctgccgcaca
     6721 cgatcaacac cgcgatccag ccgatcccgc tggctcgcag tacggccgaa ggcgacgctc
     6781 gcatcgtgtc gatgatgtac cgcctctcct cgcggcccac gccaacgctc tggctgaacg
     6841 gacaataccg cctctacgac tacgacaacc agatgccggt gttcgcctac agtcagtacg
     6901 tgcgtatgga cggcgccgcc tatagctcgc cgaccggagc aaacgatctg ttctcgatga
     6961 cccggcagtt tgcggacgtg gacgcgtcgt tcacgccgtt caggttcgtc gcgttcaggg
     7021 ccggatgggg attcgagaac gacgatcgct cccaccgcta caccgagtcg acgaccgaga
     7081 acacggcgcg cttttcgatc gactcgacca gcttctcgtg gggatcggtg cggctgcagt
     7141 acgagttctc gacccgcacc ggggaggggc tcgacgagca ggtgttcagc gacctcggcg
     7201 agcaggcctc gctgcgtcag ttcgacatct cggaccgcaa ccggcatcgc gtcgccggga
     7261 tcctccaggt cgcggtctcg aacgcggtca gtgtgagcgc ctccgtctca gtcggccagg
     7321 agcaccgtcc ggagacggag atgggcctgc aggacaacga cctgaccgga ttcagcgtgg
     7381 gcgtggactt cgttcccagc gatacggtgg acttcggggt gatgtacgga ctcgacaaca
     7441 tcagcacgct gcaaaagtca cgtcaggccg accccgggcc gcagttcaac gacccgacgc
     7501 gcgactggac gaccaacatg gacgaggacg tgcacacgct gtcagccagc ctcaatcttc
     7561 cgcgaatcac ggatcgggcg gggttctacg ccatgtacga ctacgtgtac tccaataccc
     7621 tgtacgtgta cggactggcc ccgaacagca cgctggctcc agtcgagcag cttccggcct
     7681 tgacgagcgg cttccaccgg gggtcggtgg ggttcgacta ccgactcacg gggcgggcca
     7741 cgcttggcgt cggctaccgg ctcgattcgt gggaggtgag cgacttctct cgctcgcctg
     7801 cgacactgaa cacgccgctc atcaacgcgt tcgtcaacac gctctatcag tgggcgccct
     7861 acaacgtcca cacggggttt gtgagagtca gatatcgctg gtaaaggtgc gagggtgcga
     7921 gggtgcgaag gtgcaccttc gcaccgtcgc accgtgttga tgtgctactc tcgacttcgt
     7981 cttgcctccc atcgatccag tccaggtcct gctggcattc ctggtcctga tcgtttcctt
     8041 gtcggtacac gaagcagccc acgcctggtc ggccgaccgg cttggtgaca acaccgcccg
     8101 cctcctcgga cgcgtgtcgg tcaatccgat ggtgcacatc gatgtgatcg gcaccgtcgt
     8161 ctttccgctc gtatcgatgg tgtccggcct gccgctcatc gggtgggcca agcctgtgcc
     8221 cgtcgatctg cggaatctgg gcatcgactg gcggcggaaa tggatgctcg ttgcggcggc
     8281 gggaccggcc agcaatctgg tgattgccgc cgccgcggcg atcgtcttcc ggctgatgcc
     8341 gggcggcggg ctcgacggcg aggcatttgc gagcgggtcg ccggtgtcgc gcgtcctcct
     8401 gcggctcgtg gacctgaacg tgctgctggc tgtgttcaac atgatcccgg tgccgccgct
     8461 cgacggcggc aacgtgctgt ccggcctgct aggcgggccg gcggcggcag ccttcgacag
     8521 actgcgtccc tacggtttcc tgattctcta cgggctgctg ctgaccggcc tgctgtggac
     8581 gctgatcgat ccgttgcaca cggcggccct gcgctggtta ctgtgacgga tacgaacgtt
     8641 ggacgtcccc gtgtggtgtc aggcatgcgg ccgaccggga agctgcacct cggtcacctg
     8701 gtcggcgcgc tcaggaactg ggcggcgctg caggagcgat acgactgcta ttactttgtc
     8761 gccgactggc acgccctgac gagcgagtat gcggatacag ggccgctcgc cgccaacgcg
     8821 ctcgacaacg tggccgactg gatcgccgcc ggcgtcgatc ccgagcggag cacgatcttc
     8881 gtccagtcgc tcgtgcccga gcatgccgag ctctcgctgt tgctgtcgat ggtcgtgccg
     8941 attccgtggc tcgagagggt tccgacctac aaggagcagc aggaacagct ggccgacaag
     9001 gacctgtcga ccctcggctt tctggcgtat ccgctcctgc agacggccga cattgcgatc
     9061 tacgacgggc ggttcgtccc cgtgggtgaa gaccaggtcg cgcacctgga gctctcgcgc
     9121 gaagcgatca ggcgcttcca tcagttctac ggcgaggtcc tggtcgagcc gcagccgctg
     9181 ctgacggcca ccccgcgcct tcccggcatc gacaaccgga agatgagcaa gagctacggc
     9241 aacacgatcg acctcaccga cgacgacgag acggtggtga agaaggtccg gcagatgtac
     9301 accgatccga agaggatcag ggccgacatt cccggcaccg tcgaggggaa tccggtcttc
     9361 atgtaccatg atgcgttcaa cgacgacgcc ggagagatcg aggatctgaa ggcgcggtac
     9421 cgcgccggca aggtcggtga cgttgaagtg aagaccaagc tggcccgcgc cctgaactcg
     9481 acgctcgagc cgctgcgcca gcggcgtcgt gaggtcaatg cgcggccgga tcgtatccgc
     9541 gagatcctgg tcgaagggtc gcggaaggcg cgcgttgtgg cccaggcaac gatggagcgc
     9601 gtccgggacg cggtgagatt gaggtactga tgaaggccga cggccacgag ggcttgtccg
     9661 ccgaagccct cggggcgaag gtggagcgcg accacgcgag ggagcatccc gacttcgaat
     9721 cgtctccaga cgcctatcgc gtcaagctgg aggcgttcga gggaccgctc gacctgctgt
     9781 tgcatctcat tcgcacgaac gagatgaaca tctatgacat tcagatctcg ctcatcacgg
     9841 agcagtatct cgcctatgtg gagctgatgc aggatctcga cctcgacgtg gcgggcgagt
     9901 tcctcgtgat ggcggccacg ctcatccaca tcaagtcgag gacgctcctc ccgcggccgg
     9961 atcccgctca ggacgacgag ccggaagagg acccgcgcga agcgctggta cgccggctgc
    10021 tggagcatca gaagtacaag gccgcggcgg ggctgctcca cgagcgcgag ctgctgcgca
    10081 gcgcgcagtt catgcggccg gacggccgcg tggccgagat cgccggagaa gagtacgagc
    10141 cggagctgga ggtggacctc ttcagcctgc tggcggcgtt caagggagtg ctcgagcgcg
    10201 ccaaccgccg gggcgccatg gtgctgccgg cggagcagac gtcgatcgag gaccggatca
    10261 agcagctgct cgcacggctg tccgaaacgg aggcctgcgg gttcgaggat ctcttcaacg
    10321 acggcgacgg gaccagggcg ttcgtgatcg tgacgttcct cgcgatgctc gagatgatcc
    10381 ggctgaagtt gatccgcgtg ttccagtcgg gcggggccgg gccgattcgc gtctacaagc
    10441 gtgcaaggcc cgtggacgcg ccgcatccga tcgacgaccc gctgggtccc tgacacagac
    10501 atgcctaacg acgtattcga atcagacccg ccggcctcgg ccgtcgtctc cgccgaggtg
    10561 aaggccgtcg tggaggcgct catcttcgcg tctcccgagc ccatcacgcc gaagatgctc
    10621 ttcaagctcc tggcggagga gccgaaggag gacgtcctcg ccgcgatcga cgcgctgagg
    10681 accgactacg agaaccgacc cggcctgcag atgctggagg tggcgggcgg ataccagatc
    10741 gtcacgcggc cggatttgaa cgactgggtg cgccggctgt tccacgagcg ctcgacgcag
    10801 aagctgaccg cgcaggcgct cgagtcgctc gccgtgatcg cctacaagca gccggtgacg
    10861 gcgctcgaga tcggcgagat ccgcggcgtg aacacggccg gcgtgctctc gacactgctc
    10921 gagcgccact tgatcaagat cgtcggccgc aagaacgtcg tcggccgtcc attcctctac
    10981 gccaccacga gagagttcct gatccggttc gggctgaagg acctgaacga cctgccgaag
    11041 gtggaggaca tggccgaggc gctcggcttc gatccaccgg ccggtcttgc cgagacgtcg
    11101 ccgcctgagg aaatgctgcc catcgaagac gatggcgaga gttgaaacct cgaagactta
    11161 ct
//
LOCUS       DBNGSH010000456         4968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_212202, whole genome shotgun sequence.
ACCESSION   DBNGSH010000456 DBNGSH010000000
VERSION     DBNGSH010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_212202"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="AC16CY_10655"
     CDS             <1..528
                     /locus_tag="AC16CY_10655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3354293.1"
                     /translation="PIVPREIRRVYEGRIFTVWIETIPLPKGGELQAEIIRHPGSVVI
                     IPVTASGDIVLVRQYRHAIGRRAWELPAGSLKPGEDVERAARRECQEETGSIPSRIER
                     LGSFFPTPGYCDEEMTFYKATGLRPPGPGDEDAHQDEDEDLEARTFAPDVLRQMVASG
                     EIVDLKTIAGLWLLG"
     gene            complement(536..2980)
                     /gene="lon"
                     /locus_tag="AC16CY_10660"
     CDS             complement(536..2980)
                     /gene="lon"
                     /locus_tag="AC16CY_10660"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3354294.1"
                     /translation="MSDRNDLLDLGEGPAADRPFVVPDELPILPLRDTVLFPNSFMPL
                     AVARESSVRLIDEAMSGGKLIGVFTQRDGAVEEPRQRDLHVIGTASHVHKMFKLPDGS
                     LRLIVQGLSRLTLDEITEARPYLRGRVTPAVEALDEGDRLEIDALLRNIKTNFQQVVS
                     LSPLLSDDLQTLASNMSEPGRLADFIASNLTTITTAVKQEVLETLDVRARMDALNRVL
                     IKELEVLELGSKIQSQVQSEVGRNQREYFLREQLKAIQKELGEGDDQTKEIEELREKI
                     DASGMPDGVRREALRELDRLSRMPVAAAEYTVSRTYLDWLVMLPWQKRTEEVIDLPRT
                     KDVLDVDHSGLEKVKDRILEYLAVRKLKPEVKGPILCFVGPPGVGKTSLAKSIARALG
                     RKFVRVSLGGMRDEAEIRGHRRTYIGALPGQIVQGLRRAESRNPVFILDEIDKLGSDF
                     RGDPASALLEVLDPEQNNTFRDHYLDVAFDLSEVLFITTANVLDPVPPALRDRMEVLE
                     IPGYTEEEKLAIARDHLVQKQVDNHGLTREQITITDEAIRTVIRGYTREAGVRNLERG
                     IGALCRKAARRRAEGDQSALVITTPVVTELLGAATFLDEEIEERTKDPGVAIGLAWTP
                     AGGDVLFVEASRMPGTGTLTLTGQLGEVMKESARAALSWFRAHAAAYGADPDFFKNAE
                     IHLHVPSGAIPKDGPSAGVTITVALASELSKRPVRGDIAMTGEVTLSGRVLPVGGIKE
                     KVLAARRAGIREVILPKHNDRNVKEDLSEDLRRDVTIHYVQTVDEALLLALLPATKVR
                     GDRKTARRMPAPAPVQ"
     gene            complement(3136..3585)
                     /locus_tag="AC16CY_10665"
     CDS             complement(3136..3585)
                     /locus_tag="AC16CY_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3354295.1"
                     /translation="MATTTKRKAAAKASRYDELKRMLEDRRRELLNAVHDKIRDARAD
                     GNKDRDVLDQGESSEVDIQEDIEFALIQLKSETLTKVDAALRRLEEGTYGNCFECGDE
                     IAEARLRALPFAMRCKDCEEARETAERRERMLAQKRGSSALFYDLPN"
     gene            4180..4731
                     /locus_tag="AC16CY_10670"
     CDS             4180..4731
                     /locus_tag="AC16CY_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354296.1"
                     /translation="MEIACPADCAYLSAAREHPAAVVKKQQERDVAMVLPAISHLTER
                     QHQLFFLFHSVVARHKPEGFARLIDEDVAEAARAVAATLETAARGVLYEHHPTSATAQ
                     RLARDLTAMLAELRSQGAKVYDGEAAIALRAVERAARDVRTSATQDDAYISLMARLLR
                     TSSLQRPDDGDAQAKPAGTLILP"
     gene            4843..>4968
                     /gene="rpmB"
                     /locus_tag="AC16CY_10675"
     CDS             4843..>4968
                     /gene="rpmB"
                     /locus_tag="AC16CY_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR3354297.1"
                     /translation="MAKRCDVCNKGPVVGRTISHAHNVGPRRFEPNLQTVRAIVNG"
BASE COUNT          824 a         1660 c         1581 g          903 t
ORIGIN      
        1 ccgatcgtcc cgcgcgagat tcgccgggtc tacgagggac ggatcttcac ggtttggatt
       61 gaaacgattc cgctgccgaa gggcggcgag ctgcaggccg agatcatccg tcacccgggc
      121 tcggtcgtga tcatcccggt gaccgcgagt ggcgacatcg tgctggtcag gcagtaccgt
      181 cacgcgatcg gccgccgcgc gtgggagctg ccggccggga gcctgaagcc tggtgaagac
      241 gtggaacggg ccgcgcgccg ggagtgccag gaggagaccg gctccatccc ttcgcgaatc
      301 gagcggctcg gctcgttctt cccgaccccc gggtattgcg acgaggagat gaccttctac
      361 aaggcgacgg gactgcggcc gcccggcccc ggcgatgaag acgcgcacca ggacgaggac
      421 gaggacctcg aggcacggac cttcgcgccc gacgtccttc gacagatggt ggcatcgggc
      481 gagatcgtcg atctcaagac gatcgccggg ctctggctcc tcgggtgagg caaggtcact
      541 gcacgggggc gggggcaggc atcctccgcg ccgtcttccg gtcgccccgg accttggtgg
      601 ccggcaggag ggcaagcagc agcgcctcgt ccaccgtctg cacgtagtgg atggtgacgt
      661 cgcgccgcag atcctccgag aggtcctcct tcacgttccg gtcgttgtgc ttcgggagga
      721 tgacctcgcg gattcctgca cgccgcgccg ccagcacctt ctccttgatg ccccccacgg
      781 gaagcacgcg gcccgacagc gtgacttctc ccgtcatcgc gatgtcgccg cgtaccggcc
      841 gcttcgacag ctctgatgcg agcgccaccg tgatcgtcac tccggccgac gggccgtcct
      901 tggggatggc gcccgacggc acgtgcagat ggatctcggc gttcttgaag aagtcggggt
      961 ccgcgccgta ggcagccgca tgcgcgcgga accacgacaa ggcggcgcga gccgactctt
     1021 tcatcacctc gccgagctgc cccgtcagcg tgagcgttcc ggttcccggc attctcgatg
     1081 cctcgacaaa cagcacgtcg ccccccgccg gcgtccaggc gagcccgatc gcgacgcccg
     1141 gatccttcgt gcgttcctcg atctcctcgt ccaggaacgt cgcggccccg agcagctcgg
     1201 tcacgacggg agtggtgatg accagggccg actgatcgcc ttccgcccgg cggcgagcgg
     1261 ccttccggca caaggcgccg atcccgcgct ccaggttgcg gacgcccgcc tcgcgcgtgt
     1321 agccgcggat gactgtacgg atcgcctcgt cggtaatggt gatctgctcg cgcgtcaagc
     1381 cgtgattgtc cacctgcttc tggacgagat gatcacgcgc gatggcgagc ttctcttcct
     1441 ccgtgtaccc ggggatctcg agcacctcca tccggtcacg gagcgcgggc ggcaccgggt
     1501 cgagcacgtt cgccgtcgtg atgaacagca cttccgacag gtcgaaggcc acgtccaggt
     1561 agtgatcgcg gaacgtattg ttctgctccg ggtcgagcac ctcgagcagc gccgatgccg
     1621 ggtcgccgcg gaagtccgag ccgagcttgt cgatctcgtc caggatgaag accgggtttc
     1681 gagactcggc gcggcgaagg ccctgcacga tctgaccggg caaggcgccg atgtaggtgc
     1741 ggcgatggcc gcgaatctct gcttcgtcgc gcatcccgcc gagcgacacg cggacgaact
     1801 tgcgtccgag cgcccgcgcg atcgacttgg ccagcgacgt cttgccgact ccgggagggc
     1861 cgacaaagca gagaatcgga cccttcacct cgggcttcag cttccggaca gcgaggtact
     1921 cgaggatgcg gtccttgacc ttctcgaggc cggagtgatc gacgtccagc acgtccttcg
     1981 tgcggggaag atcgatcact tcctcggttc gcttctgcca cgggagcatc acgagccagt
     2041 ccaggtacgt tcgcgagacg gtgtactcgg cggcggcaac cggcatccgc gagaggcggt
     2101 cgagctcgcg cagcgcctcc ctgcggacgc catcgggcat tcctgacgcg tcgatcttct
     2161 cgcggagctc ctcgatctcc ttcgtctggt cgtcgccttc gccgagctcc ttctgaatcg
     2221 ccttgagctg ctcgcggagg aagtactcgc gctggttgcg cccgacttcc gactggacct
     2281 gcgactggat tttcgacccc agctcgagga cttcgagctc cttgatgagg acgcggttca
     2341 gcgcgtccat cctggcgcgc acgtccagcg tctcgaggac ctcctgcttc accgccgtcg
     2401 tgatcgtcgt cagattggag gcgatgaaat ccgccagccg gccgggctcc gacatgttgg
     2461 aggcgagcgt ctgcagatcg tccgacagga gcggcgagag cgagacgacc tgctgaaaat
     2521 tcgtcttgat gttccgcagc agtgcgtcga tctccagcct gtccccttcg tccagcgcct
     2581 cgacggcggg cgtgacgcgg ccccggaggt acgggcgtgc ttccgtaatc tcgtcgagcg
     2641 tcagccggga caggccctgc acgatgagcc gcaggctgcc gtcgggaagc ttgaacatct
     2701 tgtgcacgtg actcgccgtg ccgatcacgt ggagatcgcg ctgccgcggc tcttcaacgg
     2761 cgccgtcgcg ctgcgtgaac acgccgatca gcttgccacc cgacatggcc tcgtcgatca
     2821 ggcggaccga ggattcgcgc gcgaccgcaa gcggcatgaa ggagttggga aacagcaccg
     2881 tgtccctgag cgggaggatg ggcagctcgt cgggcacgac gaacgggcgg tcggccgccg
     2941 gaccttcgcc cagatccaga agatcgttgc gatcgctcat ggcagggatg ctctcctact
     3001 tcggtgagac gcgcaagaaa aagcccaact cccaatggct gggagttggg cctcgaaaac
     3061 ccggagtgct cagctggggc ctgggccact gaggcctggg ccactgaggc ctgggccctg
     3121 aggcctgagg ccttcttagt tgggcaggtc gtaaaacagc gccgacgaac cgcgcttctg
     3181 cgccagcatg cgctcgcgcc gctcggcagt ctcgcgcgct tcctcgcagt ccttgcagcg
     3241 catcgcaaac ggcagggcgc gcaggcgcgc ctcggcgatt tcgtcgccgc actcgaagca
     3301 gttgccgtag gtcccctctt ccaggcgacg cagcgcggca tccaccttcg tcaacgtctc
     3361 ggacttcagt tggatgaggg cgaactcgat gtcttcctga atatcgacct cggagctctc
     3421 cccctgatcg aggacgtcac gatccttgtt gccgtccgct cgcgcgtcgc gaatcttgtc
     3481 gtgcacagca ttcagcaact ctcgacggcg gtcctcgagc atccgcttga gctcgtcgta
     3541 gcggctcgcc tttgccgctg ccttcctctt cgtcgttgtc gccatgatgc ccaccgctcc
     3601 cttcctacgc cgctcgatta tcgttatcta gtattgccgc cgctatgcaa tcgtcgtacc
     3661 gtgcaatctt gcctcgaggc caatggatga atccgaggct cggtcagccc agaagaacga
     3721 cgaacggtaa agtgagttat gtcgctaacc tgttccctta tctaggattt agtctgacga
     3781 ccccggttcc cttcgagccc gtcagctgat tagacgctcg ccgggttgca caggttttcg
     3841 gaagctgtcc tcaagcgacg acagaccgaa tctggcgcaa tcgttgtcgt acactcacaa
     3901 atacgtcgtg tagccatgga gaaatttcca gtcctccggt ccaggcacgt cctcgggacc
     3961 agatccccaa cgtcgagtgt agaaaaacag acactgtgat gaaatatcgc cggagcttcc
     4021 cgggacatct tcgggcgtca accgcgcgtt gcttcggccg cggaggtgtg caaagctcgc
     4081 ctcgtggtct gcccctcgtg cgggacgcgg aagggtcggc gcgagtgtcc ggccctcggc
     4141 cggcgcatct gcacgatctg ctgcggaacc aaacgcctgg tggagattgc gtgccccgcc
     4201 gactgcgcct atctctcggc ggcgcgcgag caccccgccg ccgtcgtgaa aaagcagcag
     4261 gaacgcgacg tcgcgatggt tcttcccgcg atcagccacc tgacggagcg gcagcatcaa
     4321 ctcttctttc tcttccacag tgtggtcgcc cgccacaagc ccgagggatt tgcccgcctc
     4381 atcgacgaag acgttgccga agccgctcgc gccgtcgcgg caacgctcga gacggctgcg
     4441 cgaggcgttc tgtacgaaca ccaccctact tcggctacgg cgcagcggct cgcgcgagac
     4501 ctgacggcaa tgctggccga gctccgttcg cagggcgcga aggtgtacga cggcgaagcg
     4561 gcgatcgccc tcagggcggt cgaacgggcg gcacgggacg tccgcacgtc ggcgacgcag
     4621 gacgacgcct atatctcgtt gatggcgcgc ctgctccgca cctcgtctct tcaacgcccc
     4681 gacgacggtg acgctcaggc caaaccggcc ggaacgctca ttctgccgta ggtctggtac
     4741 aatttgcggt ttgcaactcc cgacctccaa ctcccaactc ccaaacgccg tttcttggga
     4801 attggaggtt gggcggtggg agttgactct tgagaggatc ccatggcgaa acggtgcgac
     4861 gtctgcaaca agggtccggt cgtcgggcgg acgatttccc acgctcacaa cgtgggtccc
     4921 aggcggtttg aacccaacct tcagaccgtc cgagccatcg tcaacggc
//
LOCUS       DBNGSH010000457         1822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3268649, whole genome shotgun sequence.
ACCESSION   DBNGSH010000457 DBNGSH010000000
VERSION     DBNGSH010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3268649"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="AC16CY_10680"
     CDS             <1..743
                     /locus_tag="AC16CY_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354298.1"
                     /translation="RMDGPPEEVRPGLTHAQYATDPGRFVADGNRTSLVVDPPDGRIP
                     PITAEGRARQARAPARNNTVNASWLDRGNQERCITYGLPSASLPTLYNNNIQIVQSPD
                     SAVIVHEMIHEARVVPLDGRSRLNENVRGWIGFTRGWWEGDTLVVETTNFNGKNSYRG
                     STTGLHLVERYTRVAEDRLELRLTVSDPTTWERPWTVLLPMRPTEGELIEYACHEHNL
                     SMFNLLEVARDAEKAAASGRAPVSQPER"
     gene            774..1193
                     /locus_tag="AC16CY_10685"
     CDS             774..1193
                     /locus_tag="AC16CY_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354299.1"
                     /translation="MDRAPVIAFAAIMVASLASPVRGHHSFAAEFDANQPITLKGTVT
                     KMEWVNPHTWMHIDVKNPDGTVTKWMIEGGTPNTLVRRGFTKASLLPGTEITIQGYRA
                     KNGANRANGADMILPDGRRLFLGSSGTGAPKDPGDGR"
     gene            1233..>1822
                     /locus_tag="AC16CY_10690"
     CDS             1233..>1822
                     /locus_tag="AC16CY_10690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR3354300.1"
                     /translation="MSAIDRSSRRRLLQLIASSSLLPYLGLPDAWSRAAAAQLSPSSA
                     TGDLITSAAEALSVLDFEPAARAKVPAWHWAWLSTGGDDGGTIRANREGFERYQLRAR
                     RLVDVSKTDMSVRLFGRSWETPIFLCPVAGHRTYNAQGELTTARAARSRRHLQVLSTM
                     TTTAVEQVNEARGEPVWFQLYHRDDWGQTRELLKRV"
BASE COUNT          322 a          608 c          619 g          273 t
ORIGIN      
        1 ggcggatgga cggcccgccc gaggaggttc gcccgggcct cacgcacgcg cagtacgcga
       61 ccgatccggg gcgcttcgtc gccgacggca atcgcacgtc gctcgtcgtc gatccgccag
      121 acggaaggat tccacccata acggccgaag gccgcgcccg gcaggctcgc gcgcctgccc
      181 gtaacaatac cgtcaacgcg tcgtggctcg accgtggcaa ccaggagcgc tgcatcacct
      241 acgggttgcc gagcgccagc ctgccgacgc tgtacaacaa caacatccag atcgtgcagt
      301 cgccggacag cgccgtgatc gtgcacgaga tgattcacga ggcacgggtc gttccgctcg
      361 atggccgttc gcggctcaac gagaacgtcc gcgggtggat cggattcacg cgcggctggt
      421 gggaaggcga cacgctggtc gtcgagacga cgaatttcaa cggcaagaac agctaccggg
      481 ggtcgacgac cggcctccac ctggtcgagc gttacacacg cgtcgccgag gaccggctcg
      541 agctccggct gacggtgagc gacccgacga cctgggagcg gccgtggacc gtgctgctgc
      601 cgatgcggcc gaccgaaggc gagttgatcg agtacgcgtg ccacgaacac aacctgagca
      661 tgttcaacct cctcgaggtt gcacgtgacg cagagaaggc agccgcctcg ggccgggcac
      721 cggtgtcgca accggagcgt tgatccgggt ttactagcag gggaccagga gccatggata
      781 gagcaccagt catcgcgttt gccgccatca tggtcgcgag cctcgcctcg ccggttcgcg
      841 ggcatcattc gtttgccgcg gagttcgacg ccaaccagcc gatcaccctc aagggtacgg
      901 tgacgaagat ggagtgggtg aaccctcaca cctggatgca catcgacgtc aagaacccgg
      961 acggtacggt gaccaagtgg atgatcgagg ggggcacgcc caatacgctc gtgcgccgcg
     1021 gcttcacgaa ggcgtcgctg ctgccgggca ccgagatcac gatccagggg tatcgcgcga
     1081 agaacggcgc caatcgcgcc aacggcgcgg acatgatcct gcccgacggc cggcggctct
     1141 tcctgggctc gtcgggcacg ggcgcaccga aggacccggg cgacgggcga tagagcgggg
     1201 ttttcaccgg cgcgttcgcg gcgaaacgac tgatgtctgc catcgacagg agcagccggc
     1261 ggcggctgct ccagctgatt gcctccagct ctcttctccc gtatctcggc ctgcccgacg
     1321 cctggtcgcg cgccgcggcc gcacagcttt caccgtcgtc cgcgacaggc gacctgatta
     1381 cgagcgccgc tgaagcgctg tccgtcctcg acttcgagcc ggcggcccgc gcgaaggtcc
     1441 ccgcgtggca ctgggcgtgg ctgtcgaccg gcggcgacga cggcggaacg atccgggcaa
     1501 accgtgaggg cttcgagcgc tatcagctcc gcgcccgtcg tctcgtcgac gtcagcaaga
     1561 cggatatgtc ggtgcgcctc ttcggtcgct cctgggagac gccgatcttt ctctgcccgg
     1621 tggccggcca tcgcacctac aacgcgcagg gcgagctcac cacggcgcgc gcggccagat
     1681 ccaggcgcca cctccaggtc ttgtcgacga tgacgacgac cgcggtggag caggtgaacg
     1741 aggcgcgtgg agagcccgtg tggtttcagc tgtatcaccg ggacgactgg ggccagacgc
     1801 gcgagctgct gaagagagtc ga
//
LOCUS       DBNGSH010000458        10263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2589692, whole genome shotgun sequence.
ACCESSION   DBNGSH010000458 DBNGSH010000000
VERSION     DBNGSH010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2589692"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="AC16CY_10695"
     CDS             <1..474
                     /locus_tag="AC16CY_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3354301.1"
                     /translation="YDRHAGKVLGLARRILRDDGDAEDVVQEVFSQAWRTATRYAAVR
                     GTVAGWLLMIARARAIDRLRARRARPDVTAEPLADTVPSETVPLTDQLVAAEQAARVR
                     AALTALPPEQRIALELAYFEGLTQSEIAERLRTPLGTVKTRMRTALAALRRSLRP"
     gene            471..1313
                     /locus_tag="AC16CY_10700"
     CDS             471..1313
                     /locus_tag="AC16CY_10700"
                     /inference="COORDINATES: protein motif:HMM:NF052375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3354302.1"
                     /translation="MTMNPHDTIAARLDEYALGQLSPEERHDVEVHVRECETCSADLR
                     ELTLVMEHLARTAGESTPPAALRTRVLDGLASQPQEPPLSAGGGPDARVVALPPARTA
                     ERSRPAIGWLAAAAAVVVALGGLLYYSSASRQRLASELRGAEVTVTDLERRLAEYTGQ
                     ADLAVSILTAGDMRSMALARPDAAGVSTARAYWSPTRGLLVVADQLPAPPPGRIYQVW
                     LIGSGQQPLSAGLLGAQGTARGMLIAPPPAGLAPGAVTVAVTDEPPGGLAAPTGSMSL
                     VGSI"
     gene            1429..3252
                     /locus_tag="AC16CY_10705"
     CDS             1429..3252
                     /locus_tag="AC16CY_10705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3354303.1"
                     /translation="MSASQSVPARLSADKGRGTLQDETLAGVAAPIPTLRPSASALIA
                     QEAFGADPSLYAIAVVDDHGTPLGLINRFRFAETLSQQADPDTLANRPVGMIMDRTPL
                     VLDEHTPIDQLSLILSDDHSKYIFDGFIVTQNGRYLGVGTGYSLMRRLTERRQSALAQ
                     LAYHDTLTGLPNRQLFLDRLTQALATAFRNRRRVAVLYLDIDRFKSINDEFGHTIGDL
                     LLQQTADRFRAVVRAQDTVARLSGDEFAFILTELQAAEHAELVAQKLTNLVRQPYALD
                     GHEVNICCSVGVAVYPEHTENQPTLMRMADDALYCAKRTRNTVQAYSAAMARPVSSGP
                     LLFNSVRKAIDLGQLEVHYQPQAELRGGALCGLEALVRWRQPGGDLVSTHELIQIAEE
                     AGLISEITDFVCGASMNQLRQWHVEGLARGLRLSINISGAEVQDGVLSPMLRGHVQAS
                     GLSMSMIELELTESGLMRSDAYATQLLSELRGEGVRVSVDDFGTGYSSLGRLQRLPVD
                     VLKIDKTFVDDIDAGAKQGALVRAIVVMAHSLGLTVVAEGVETELQRAFLEEHGCDIY
                     QGHLLSPPLPAQEMARYLRLNTPSASKPASPARPRRRSAQR"
     gene            3380..5524
                     /gene="glgX"
                     /locus_tag="AC16CY_10710"
     CDS             3380..5524
                     /gene="glgX"
                     /locus_tag="AC16CY_10710"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR3354304.1"
                     /translation="MKLKPGAPYPLGATWDGSGVNFAIFSEHATKVELCLFDSPDSVR
                     ESTRLTLTEQTDLIWHGYLPGILPGQLYGYRIHGPYDPEHGHRFNPNKVLLEPYAKAI
                     GRPIRWSDEMHGYEYGDPKEDLAIDTRDSAPFAPLAAVIDPAFTWGEDRRPRTPWHET
                     VIYELHVKGFTRRHPRLPEGMRGTYTGLASDEAIRHLQNLGVTSVELMPVHHHAYDRR
                     LVERHLSNYWGYNTSAFFAPDTRFAMNHSSSGSVREFKTMVRNLHAAGIEVILDVVYN
                     HTAEGDHLGPTISLRGVDNAAYYRLKAGCPRFYEDFTGCGNTLNMQHPRVLQLIMDSL
                     RYWILEMHVDGFRFDLASALARELHAVDRLGAFFDIIHQDPVISQVKLIAEPWDLGEG
                     GYQVGNFPVGWAEWNGRYRDSIRRFWRGDPGLISELATRIAGSSDLYDHSGRRPHASV
                     NFVTCHDGFTLHDLVSYNEKHNEANLEGNHDGEVHNHSFNYGVEGPTSDPKIRAVRER
                     QKRNFLATVFFSLGVPMLYAGDEMGRTQRGNNNAYNQDNEISWLDWQRSADQDDLLRF
                     TEYVIALRRSQPVLKRRRFFQGEAVRGGPTKDITWFAPDGSEMSDDDWRQEDLRSLGV
                     RLDGRLIDETDAQGNPIVGDTLVLLFSADEVGMDFTLPAPGPDQHWERLLDTSDPRWS
                     KRTVHDEPRYPLQPRSVVVFRLAGEPPNGASE"
     gene            5521..7179
                     /gene="treS"
                     /locus_tag="AC16CY_10715"
     CDS             5521..7179
                     /gene="treS"
                     /locus_tag="AC16CY_10715"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HIR3354305.1"
                     /translation="MNGAANGSADPDLLWYRDAVIYQLHVKTFADSNSDGIGDFKGLT
                     GKLDYLRDLGVDCLWLMPMYPSPFRDDGYDIADYYSIHPSYGTLDDFREFLDAAHARR
                     LRVVTELVLNHTSDQHAWFKQARSARDDPRRDWYVWSDTDDRYSGVRIIFLDTERSNW
                     AWDPQSKAYYWHRFFHHQPDLNYDNPAVREEMWKVMSFWLELGVDGFRVDAVPYLIER
                     EGTSCENLPETHAVIRWLRSQLDAHFPGRMLLAEANQPPELVRPYFGEGDEFHMAFHF
                     PLMTRMFMAVRLEDRKPLVDVVERTPEIPATCQWGIFLRNHDELTLEMVTEIEREFMY
                     EEYARDPVSRLNLGIRRRLAPLLEGDRRRIELMNGIVMSMPGSPIIYYGDEIGMGDNI
                     YLGDRNGVRTPMQWTGGWNAGFSTADPERLAQPLISNPLFGYQAVNVESQLRADHTLL
                     NWMRRLVSVRKSSHVFSRGSTTFLKPANHRVLAYLRTLGKEQVLVVCNLSSSAQAVEL
                     DLRSLAGALPIEMFGKSMFPRIGELPYILTLGPYDFFWFRLRRV"
     gene            7261..8544
                     /locus_tag="AC16CY_10720"
     CDS             7261..8544
                     /locus_tag="AC16CY_10720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3354306.1"
                     /translation="MEEALAEGSLLSDELLRHLMAVGQVDVLVGVPTLNNASTIGGVV
                     NAVHQAFGKYFPRDRTVLINSDGGSVDGTQDVIRGRSQDQTETVTVSHALRTTHSIST
                     PYHGVPGKGSALRQIMTAADLAQAKAVAVLDADVASATPEWVASLVQPVRNQQFDYVA
                     PIYSRHPLDSPLITQLVRPLFRAASGWQVREPLAAEFGCSNRFLAHCLEQNVWDNELA
                     RYGIDLWLTATALYGGFRCCQTMVGPRVAAAAPGRPGFREVFQQVVGSVFLLLEQQAS
                     SWVTRVGSEQIPIAGPAADGKGDGQAPGVSRLGSTFRDDLRNLESVLRAILGEGTLRE
                     LNAIAEAGVEEIRYPDALWVRTVYEFMGAFHRGVMRREHVTQALIPLYLGRTGSFLVE
                     QSSRNPAEVEGALEALCVEFERAKPSLVQYWNQAT"
     gene            8608..9309
                     /locus_tag="AC16CY_10725"
     CDS             8608..9309
                     /locus_tag="AC16CY_10725"
                     /inference="COORDINATES: protein motif:HMM:NF017373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3354307.1"
                     /translation="MAQMIADAVSQATRNFVDSLAIFLPKLVTTISIIVVGWLIAIVL
                     RTAVRTVLRWLSFNAWAERSGVASLLKSAGLPVADVAVGAMVFWLVFLGFLLSGIDVL
                     GFPALQGLLAGFAAFVPRLLVALVILIVAFVVANFAWRATLLAAVNARIPSPRLLSGA
                     VRWLILILAGAMALEQIAVARTVVLTALAIAFGAVMLGIAIAFGLGGRGIARRILEHQ
                     FPERERSGVDEISHL"
     gene            9306..9500
                     /locus_tag="AC16CY_10730"
     CDS             9306..9500
                     /locus_tag="AC16CY_10730"
                     /inference="COORDINATES: protein motif:HMM:NF025614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3354308.1"
                     /translation="MREDTDTFTVQCPYCGEMVEIYVERDVRGTFVQDCEVCCSPWRV
                     RVFREGGQRQVELGRADGSE"
     gene            9523..9720
                     /locus_tag="AC16CY_10735"
     CDS             9523..9720
                     /locus_tag="AC16CY_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354309.1"
                     /translation="MAGTSDASRSDGVRKPVSRQRATGIAGVGGAGEERGRQTIATAE
                     RRRTIRRALEMPVAGRSDPPR"
     gene            complement(9779..>10263)
                     /locus_tag="AC16CY_10740"
     CDS             complement(9779..>10263)
                     /locus_tag="AC16CY_10740"
                     /inference="COORDINATES: protein motif:HMM:NF019157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR3354310.1"
                     /translation="NDPNWDARTFDLDRDLKVVEEKVGAIVDAVNPDLRAFKARGGKL
                     LMYHGWNDTAISAGNAVNYYSSVLEKMGQKQDDWIRLFMAPGMQHCGGGPGPSQANWM
                     GVLERWRESGKAPDRIDAARVANNRVDMTRPLCPYPQVATYTGVGSTNDAANFVCKAP
                     "
BASE COUNT         1722 a         3507 c         3372 g         1662 t
ORIGIN      
        1 tacgatcgcc atgcgggcaa ggtgctcggc ctggctcgac ggatactccg cgacgacggc
       61 gacgccgagg acgtggtgca ggaggttttt tcgcaggcat ggcgcacggc gacgcgatat
      121 gccgccgtac gcggaacggt cgcgggctgg ctgctcatga tcgcccgcgc gcgcgcgatc
      181 gatcgccttc gcgcgcgccg cgcgcgccca gacgtgacgg cggagccgct ggccgacacg
      241 gttccgagcg agacggtccc gctgacagat caactcgtgg ccgccgagca ggccgcccgc
      301 gtgcgcgcgg cgctgaccgc ccttccaccc gaacagcgga tcgcgctcga gctcgcctac
      361 ttcgaggggc tgacacaatc ggagatcgcg gagcggctga ggacgccgct gggcaccgtg
      421 aagacgcgaa tgcggaccgc gctcgctgcg ctgcgacgga gcctgcggcc atgaccatga
      481 acccgcacga cacgatcgcc gcaagactcg acgaatacgc cctcggacag ctgtcgcccg
      541 aagagcgtca cgacgtcgag gttcacgttc gtgaatgcga gacctgttcg gcagacctgc
      601 gcgagctgac gctcgtgatg gagcacctgg cgcgtacggc gggcgaatcg acgccgcccg
      661 ccgcattgag gacacgcgtc ctggacggcc tcgcctcgca gccgcaggag ccgccgctct
      721 ccgcaggagg agggccggat gcccgcgtcg tggcactccc gcccgccagg acggctgaac
      781 gatcgcgccc ggccatcgga tggcttgccg cggctgccgc ggttgtcgtc gcgctcggcg
      841 gcctcctcta ctactcgagt gcctcgcggc agcgcctggc gtcggaatta cgcggcgcgg
      901 aggtgacggt caccgacctc gagcgtcgtc ttgccgagta caccggtcag gccgatctcg
      961 ccgtgtcgat cctcacggcc ggcgacatgc gttccatggc gctggccaga ccggatgccg
     1021 ccggtgtctc caccgcgagg gcctattgga gtccgactcg cggacttctc gtcgtcgcag
     1081 atcagcttcc ggcaccgccg ccagggcgca tctaccaggt gtggttgatc ggcagcggcc
     1141 agcagcccct cagcgccggg ctgctcggcg cacagggaac cgcgcgcggg atgctgatcg
     1201 cgccgccgcc cgccggtctg gcacccgggg ccgtgaccgt tgcggtgacc gacgagccgc
     1261 cgggcggcct tgccgcccct accgggtcga tgagcctcgt cggctcgatt tgagccaggc
     1321 gggtctgccg tcacaggagt gcgctcgttt tcactcgaca atatatccgt ctttcacgcg
     1381 gctgcccttc tgatcggcgg ctctgccgtc gatacgatgg tcgtacatat gtcagcgtcc
     1441 cagagcgtgc cggctcgact cagcgccgac aagggccgcg gcactcttca agacgaaacg
     1501 ctcgccggcg tcgcagcgcc aatcccgacc ctgcgtccga gcgcatcggc actgattgcg
     1561 caggaagcct tcggcgcgga cccgagcctg tacgccatcg cagtcgtcga cgatcacggc
     1621 acgcccctcg ggctcatcaa ccgcttcagg ttcgctgaaa cgctgtcgca gcaggccgac
     1681 ccggatacgc tcgcgaaccg gcccgtcggc atgatcatgg accggacgcc gctggtcctc
     1741 gacgagcaca ccccgatcga tcagctcagt ctcatcctca gcgacgacca cagcaaatac
     1801 atcttcgacg gcttcatcgt cacgcagaac gggcgatacc tcggggtggg caccggctac
     1861 agcctgatgc ggcggctcac cgagcggcgc cagagcgcgc tggcccagct ggcctaccac
     1921 gacacgctca ccgggcttcc gaaccgccaa ctgttcctcg atcgactcac gcaggcactg
     1981 gcgaccgcgt tccgaaatcg ccggcgcgtc gcggtcctgt acctggacat cgatcgattc
     2041 aagtcgatca acgacgagtt tggacacacc atcggcgacc tgctcctcca gcagaccgcc
     2101 gatcgattcc gcgccgtcgt acgagcgcag gatacagtcg cgcggctgag cggcgacgag
     2161 tttgcgttca tcctgacgga gcttcaggcc gccgagcacg cggagctcgt cgcccagaag
     2221 ctcacgaacc tggtgcgcca gccgtacgcg ctcgacggcc acgaggtcaa catctgctgc
     2281 agcgtcggcg tcgccgtcta ccccgagcac acggaaaacc agcccacgct catgcgcatg
     2341 gccgacgacg cgctgtactg cgcgaagcgg acgcggaaca cggtgcaggc ctactccgcc
     2401 gccatggcgc gccccgtgtc gtccggcccg ctgctcttca actcggttcg caaggcaatc
     2461 gacctcgggc agctcgaggt gcactaccag ccccaggcgg agcttcgcgg cggcgccctg
     2521 tgcggcctcg aagcgctcgt gcgctggagg cagccgggcg gcgatctcgt ctcgacgcac
     2581 gagctgattc aaatcgccga agaggccggg ctgatctcgg agatcaccga cttcgtctgc
     2641 ggcgcgtcga tgaatcagct ccggcagtgg catgtcgaag ggctcgctcg cggacttcgg
     2701 ctgtcaatca atatctccgg cgccgaagtg caggacggcg tgctgtcgcc gatgctcagg
     2761 ggccacgttc aagcctcggg cttgtcgatg tcgatgatcg agctggagct caccgaaagc
     2821 gggctgatgc gttcagacgc ctacgcgacg cagctcctgt cggagctgcg cggagaaggc
     2881 gtgcgcgtct cggtggacga cttcggcacg gggtactcct cgctcggacg gctccagcgc
     2941 ctgcccgtgg acgtgctgaa gatcgacaag accttcgtcg acgacatcga cgccggcgcc
     3001 aaacagggcg cgctcgtgcg cgccatcgtc gtcatggcgc attcgctggg gctgaccgtc
     3061 gtggccgaag gcgtcgaaac cgagctccag cgcgcgttcc tcgaggagca cggctgcgac
     3121 atctatcagg gtcatctgct gagcccgccg cttcccgcgc aggagatggc gcgctacctg
     3181 aggttgaaca cgccctccgc gtccaagccc gccagcccgg cccgcccccg ccgacgttcc
     3241 gcccagcgct gactcacggt ccggctgaaa ccggacacta cgagcgtcta cgggcagcgg
     3301 gcagacgtcg tgtccggctt tagccggacc aattcggcga tgttaggatc accccggccc
     3361 ctatgccgtt cgccagtcga tgaagctcaa gcccggagcc ccctacccac tcggcgccac
     3421 ctgggacgga tcaggcgtca actttgccat cttctccgag cacgccacga aggtggagct
     3481 ctgcctcttc gactcgccgg acagcgtgcg cgaatcgacg cggctgacgc tgaccgagca
     3541 gaccgacctg atctggcacg gctacctgcc cggcatcctg ccgggtcagc tgtacggcta
     3601 ccggatccac ggtccgtacg atccggagca cgggcatcgg ttcaacccga acaaggtcct
     3661 gctcgagccg tacgcgaagg ccatcggccg ccccatccgg tggagcgacg aaatgcacgg
     3721 ctacgagtac ggcgacccga aggaggatct cgcgatcgac acacgcgaca gcgcgccgtt
     3781 tgcgccgctg gcggcggtga tcgatccggc cttcacgtgg ggagaagacc gccggccgcg
     3841 cacgccctgg cacgagacgg tgatctacga gctgcacgtc aagggattca cgagacgcca
     3901 tccccggctc cccgaaggga tgcgcggcac ctacaccggg ctcgcgtcgg acgaggcgat
     3961 ccgtcacctg cagaacctgg gcgtgacctc cgtcgagctg atgccggtcc accatcacgc
     4021 ctacgatcgg cgcctggtcg agcgtcacct gagcaattac tgggggtaca acaccagcgc
     4081 gttcttcgct cccgacacgc gcttcgcgat gaaccactcc tcgtccgggt ccgtgcgcga
     4141 gttcaagacg atggtgcgga acctgcacgc ggcgggcatc gaggtcatcc tcgacgtcgt
     4201 gtacaaccac accgccgaag gcgatcattt ggggccgacg atctcgctgc gcggcgtgga
     4261 caacgcggcc tactaccgcc tcaaggccgg gtgcccgcgc ttctacgagg acttcaccgg
     4321 ctgcggcaac acgctgaaca tgcagcatcc gcgcgtgctg cagctcatca tggacagcct
     4381 ccgctactgg atcctggaaa tgcacgtgga cggcttccgc tttgacctgg cgagcgcgct
     4441 cgcccgggag ctgcatgccg tcgatcggct cggagcgttc ttcgacatca tccaccagga
     4501 tccggtcatc tcgcaggtca agctgatcgc cgaaccgtgg gacctgggcg aaggcggcta
     4561 ccaggtgggg aacttccctg tcggctgggc ggagtggaac gggcgctatc gcgactcgat
     4621 tcgccgcttc tggcgcggcg accccggcct catctcggag ctggccacgc ggatcgcggg
     4681 cagcagcgac ctctacgacc acagcggccg caggccgcac gccagcgtga acttcgtcac
     4741 gtgccacgac ggcttcacgc ttcacgatct cgtcagctac aacgagaagc acaacgaagc
     4801 caacctggaa ggcaaccacg acggcgaggt ccacaaccac agcttcaact acggcgtcga
     4861 aggcccgacg tccgacccga agatccgcgc ggtacgcgag cggcagaagc ggaacttcct
     4921 cgccacggtg ttcttctcgc tgggcgtgcc gatgctctat gccggcgacg aaatgggcag
     4981 gactcagcgc ggcaacaaca acgcctacaa ccaggacaac gagatcagct ggctcgactg
     5041 gcagcggtcg gccgatcagg acgacctgct gcggttcacc gagtacgtca tcgcgctgcg
     5101 ccggagccag cccgtgctca agcgccggcg gttctttcag ggcgaggcgg ttcggggcgg
     5161 gcccaccaag gacatcacgt ggttcgcgcc cgacggcagc gagatgtccg acgacgactg
     5221 gaggcaggag gatttgcgga gcctcggagt ccggctggat ggcagactga tcgacgagac
     5281 cgatgcccag ggcaacccga tcgtcggcga caccctggtc ctgctgttca gcgccgacga
     5341 agtcggcatg gatttcactc tgccggcccc aggccctgac cagcactggg agcgcctgct
     5401 cgacacctcg gacccgcgct ggtcgaagcg gaccgtgcac gacgagccgc gctacccgct
     5461 gcaaccgagg tcggtcgtcg tgtttcgact cgccggggag ccgcccaacg gagcctcgga
     5521 atgaacgggg ccgccaacgg ctcggccgac ccggacctcc tgtggtaccg cgacgccgtc
     5581 atctaccagc tccacgtcaa gacgttcgcc gacagcaaca gcgacggcat cggcgacttc
     5641 aaggggctga ccgggaagct cgactacctg cgcgatctcg gcgtggactg cctctggctc
     5701 atgcccatgt acccgtcccc gtttcgcgac gacggctacg acatcgccga ttactacagc
     5761 atccacccga gctacgggac gctcgacgac ttccgggagt ttctcgacgc cgcgcacgcg
     5821 cgccggctcc gcgtcgtcac cgagctggtc ctcaaccaca cgtcggacca gcacgcctgg
     5881 ttcaagcagg ccaggagcgc gcgcgacgac ccgcgccgcg actggtacgt gtggagcgac
     5941 acggacgacc gctacagcgg cgtgcgcatc atctttctcg acaccgagcg gtcgaactgg
     6001 gcctgggacc cgcagtcgaa agcctactac tggcaccgct tctttcacca tcagcccgac
     6061 ctgaactacg acaacccggc cgtgcgcgag gagatgtgga aggtgatgtc cttctggctc
     6121 gagctcggcg tggacgggtt ccgggttgac gcggtgccgt atctcatcga gcgcgaaggc
     6181 acctcctgcg aaaacctgcc cgagacgcac gccgtcatcc gatggctccg gtcgcagctc
     6241 gatgcccact ttccgggacg gatgctgctg gccgaggcca accagccgcc cgagctcgtg
     6301 cgtccgtact tcggcgaggg cgacgagttc cacatggctt ttcacttccc gctgatgact
     6361 cgcatgttca tggcggtgcg gctcgaggat cgcaagccgc tcgtggacgt cgtcgaacgg
     6421 actcccgaaa tcccggcaac gtgccagtgg ggcatctttc tgcgcaacca cgacgagctc
     6481 accctcgaga tggtcacgga gatcgagcgt gagttcatgt acgaggagta cgcccgcgac
     6541 ccggtgtcgc ggctgaacct cggcatccgc cgccggctgg ctccgctgct cgagggcgat
     6601 cgcaggcgaa tcgagctgat gaacgggatc gtgatgtcga tgcccggcag ccccatcatt
     6661 tactacggcg acgagatcgg catgggcgac aacatctatc tcggcgatcg caacggcgtt
     6721 cgcacgccga tgcagtggac gggaggatgg aacgccgggt tctcgacggc ggacccggag
     6781 cggctggccc agccgctcat ctcgaacccc ctttttggtt accaggcggt caacgtggaa
     6841 tcgcagttac gcgccgacca cacgctcctc aactggatgc ggcgcctcgt gagcgtccgc
     6901 aagtcctccc acgtgttcag ccggggatcg acgacgttcc tcaagccggc gaaccatcgc
     6961 gtgctggcct acttgaggac gctcggcaag gaacaggtgc tcgtggtctg caacctgtcg
     7021 agcagcgccc aggccgtgga gctcgacctg cgctcgctgg ccggggccct cccgatcgag
     7081 atgttcggca agagcatgtt ccctcgaatc ggggagctgc cgtacatact gacactggga
     7141 ccgtacgact tcttctggtt caggctcagg cgcgtgtagg tcggggtcag atcttgattt
     7201 ctgcgctcgc ggacacgaca acccagctgg ccatcagcgc aaaaatcaag atctgacccc
     7261 gtggaggaag cattggccga aggcagcctg ctgtcggacg aactgctccg tcacctgatg
     7321 gccgtaggcc aggtggacgt cctcgtcggc gtgccgacac tcaacaacgc atccacgatc
     7381 ggcggcgtcg tcaacgcggt gcaccaggcg ttcggcaagt acttcccccg cgaccggacg
     7441 gtgctgatca actcggacgg agggtcggtg gacggcacgc aggacgtcat caggggccgc
     7501 tcccaggacc agactgagac cgtcacggtg tcgcacgcgc tgcggacgac gcattcgatc
     7561 agcacgccgt atcacggggt gcccggcaag gggagcgcgc tgagacagat catgacggcc
     7621 gccgaccttg cgcaggcgaa ggcggtggcg gtgctcgatg ccgacgtggc cagcgccacg
     7681 cccgagtggg tggcctcgct cgtccagcct gtgcggaacc agcagttcga ctacgtcgcc
     7741 ccgatctact cgcgacatcc gctcgacagc ccgctcatca cgcagctggt ccggccgctc
     7801 ttccgggcgg cgtccggctg gcaggttcgc gagcccctgg cggccgagtt cggctgctcg
     7861 aatcgttttc tcgcgcactg cctcgaacag aacgtctggg acaacgagct ggcccgctac
     7921 ggcatcgatc tgtggctgac ggccaccgcg ctctacggcg gctttcgctg ctgccagacg
     7981 atggttggtc cgcgcgtggc cgcggcggcg cccggccgcc cgggattccg ggaagtgttc
     8041 cagcaggtcg tgggatcggt cttcctgctg ctcgaacagc aggcctcgtc ctgggtgacg
     8101 cgtgtcggat ccgagcagat accgatcgct ggcccggcgg ccgacggcaa gggcgacggc
     8161 caggcccccg gcgtgagccg gctgggaagc acgttccggg acgacctgcg caacctcgag
     8221 tcggtgctcc gggccatcct cggcgagggc acgctgcggg agttgaacgc catcgcggag
     8281 gcgggcgtcg aggaaattcg ttacccggat gcgttgtggg tacggaccgt gtacgagttc
     8341 atgggcgcgt ttcaccgcgg ggtgatgcgg cgcgagcacg tgacacaggc cctgatcccg
     8401 ctctacctcg ggcgaacggg atcgtttctg gtcgagcaat cgtcgaggaa tccggcagag
     8461 gtcgaaggcg cgctcgaagc gctgtgcgtc gaattcgagc gagccaagcc gtcgctggtc
     8521 cagtactgga accaggcaac gtaaacgtgt ccggcttccc cggcctgagc gcagtcgaag
     8581 gcagccggac cgagcgcgag gtgagtcatg gcccagatga tcgcggacgc cgtcagtcaa
     8641 gccaccagga acttcgtgga ctcgctggcg atcttcctcc cgaagctggt caccacgatc
     8701 agcatcatcg tcgtcgggtg gctcatcgcc atcgtgctgc ggaccgccgt ccggaccgtg
     8761 ctcaggtggc tgtcgttcaa cgcctgggcc gagcggagcg gcgtggcgtc gctcctcaag
     8821 agcgcgggac tgcctgtcgc cgacgtcgcg gtgggagcga tggtcttctg gctggtgttt
     8881 ctcggcttcc tcctctccgg catcgacgtg ctcgggttcc cggcgctgca gggactgctg
     8941 gccgggttcg ccgcgttcgt ccctcggctg ctggtggcgc tcgtcatcct gatcgtcgcg
     9001 ttcgtcgtcg ccaacttcgc ctggcgcgcc acgctgctcg ccgccgtcaa cgcgcgcatc
     9061 ccgtcgccgc ggctcctgag cggcgcggtc cggtggctga tcctgattct ggccggcgcc
     9121 atggcgctcg agcagatcgc cgtcgcgcgc acggtggtcc tcacggcgct cgccatcgcg
     9181 tttggcgccg tgatgctcgg gatcgccatc gccttcggcc tcggcggacg aggaatcgcc
     9241 aggcggattc tcgaacacca gttcccggaa cgggaacggt ccggagttga cgagatatcc
     9301 cacctgtgag agaggacacc gacacgttca ccgttcaatg cccgtactgc ggcgagatgg
     9361 tcgagatcta cgtcgaacgg gacgtccgcg gcacgttcgt ccaggactgc gaggtgtgct
     9421 gcagtccctg gcgggtgcgc gtgtttcgcg aaggcggaca gcgccaggtg gagctcgggc
     9481 gcgctgacgg atccgaatag attcgcgtca gtaaccggac aaatggccgg cacgtctgat
     9541 gcgtccagaa gcgacggcgt tcgcaagccc gtctcgcgac agcgcgccac cggcatcgca
     9601 ggcgttggag gcgcggggga ggagcgaggg cgccagacga tcgcgacggc ggagcgccga
     9661 cggaccatca gacgtgcgct ggagatgccg gtggccgggc gcagcgaccc gccgcgatga
     9721 aggacgcatc aggcggggcg gcgatcagaa cgctgccgaa gtcggaagtt gtcgtgagct
     9781 acggagcctt gcaaacaaag ttggccgcgt cgttcgtgct gccaacgcct gtgtacgtcg
     9841 cgacctgtgg ataggggcac aggggcctgg tcatgtccac gcggttgttg gcgacgcgcg
     9901 cggcgtcgat gcgatccggc gcctttcccg actcgcgcca gcgctcgagc acgcccatcc
     9961 agttcgcctg gctcgggccg ggtccgccgc cgcagtgctg catgccgggc gccatgaaca
    10021 ggcgaatcca gtcgtcctgc ttctggccca tcttctccag caccgacgag tagtagttca
    10081 cggcattgcc ggccgaaatc gccgtgtcgt tccagccgtg gtacatgagg agctttccgc
    10141 cgcgcgcctt gaacgcgcgc agatccgggt tcacggcatc gacaatggcg ccgacctttt
    10201 cctcgacgac cttgagatcg cgatcgagat cgaaggtgcg ggcgtcccag ttgggatcgt
    10261 tgt
//
LOCUS       DBNGSH010000459         5161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1230493, whole genome shotgun sequence.
ACCESSION   DBNGSH010000459 DBNGSH010000000
VERSION     DBNGSH010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1230493"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /gene="mreC"
                     /locus_tag="AC16CY_10745"
     CDS             complement(<1..435)
                     /gene="mreC"
                     /locus_tag="AC16CY_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3354311.1"
                     /translation="MAVLDIRQRTGWLFLAVIVGHILLISAQVNTNRGVPVLEEATVG
                     VFAEVQRAASGLIGAVRSGWTDYVALRGVNEENARLREEIASLKIGLQQQRALAEQSE
                     SLQALLDLRSTTKLSTTAAAVIAGGASPEFRTITIDKGTGDGM"
     gene            complement(470..1495)
                     /locus_tag="AC16CY_10750"
     CDS             complement(470..1495)
                     /locus_tag="AC16CY_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3354312.1"
                     /translation="MPLGSFFSLFSSDLAIDLGTANTCVYARGKGIVVNEPSIVAINK
                     VNGRVEAVGKEAKDMLGRTPGNIVAIKPMKDGVIADFEVTEKMLTYFIKKAHNRNVWV
                     RPRIVIGVPSEITQVEKRAVKDSAYRAKASEVHLVEEAMAAAIGAGMPITEPSGNMIV
                     DIGGGTTDIAVISLAGIVYSKAVRVAGNEMDEAIIAYIKKAYNLLIGERTAEQIKMEI
                     GSAFPLEEKMTMEIKGRHQIEGVPKTITISDEEIREALTETVNVIVDAVRVALERTPP
                     ELSADIVDRGIVLTGGGSLLKNLDKRLREETGLPLAMAEDPLSSVVLGAGKMLSDFNL
                     LRKISID"
     gene            complement(1498..2598)
                     /locus_tag="AC16CY_10755"
     CDS             complement(1498..2598)
                     /locus_tag="AC16CY_10755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3354313.1"
                     /translation="MRSVARRDPSPRAARGTQRQDLASRLRSRVRTLRARADRRDAFV
                     EAIREANANYPPDPRKVAEWIVRQGQGWLPAQCWAVIAHDVNGHLNVLADEGLVPNLG
                     PSLWSAANWVMRHGTEFFSGDMAKDSRGGPGALGSAVALPLMSRSRSIGVLACLDSTP
                     SRSAPSMGPSLAMSLRTLLEPSAIALDNALALQKADLLSVTDDLTRLHNSRYLNSVLR
                     KEFKRAVRNGSALSVLFLDLDSFKQVNDQHGHLAGSKVLMEAAAVIRSCARETDVVAR
                     FGGDEFSLILPDTDTEGAVSVAERIRDRLRASRFLTSDGLSVHQTVSIGVATLQRDNP
                     KSADDLIKAADVAMYRVKAAGKDGILVAQEDW"
     gene            2895..4832
                     /locus_tag="AC16CY_10760"
     CDS             2895..4832
                     /locus_tag="AC16CY_10760"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR3354314.1"
                     /translation="MTMLDRMRRHKNWLKWSLAVVVLTFVLLYIPDFVQTTPTGTGAG
                     PREVIAEVDGRTLTAGDFQQRYLTQLQAYRNQLGGNVSDQLLRQLGIDQQILSQMIDE
                     QVALIEADRNGISVSDDELAQQIFAIPGLQENGRFIGEARYEQLLLNQVPPMTKAQFE
                     ESLRRSMVLDKLRGALTDWMAVSEADVEREYHQRNEKVKMQVVALTADRFRSGVSVTD
                     ADVAAHFEAHKAEYRIGEQRKIKYLVLDRDQARLKVSVPATDIQRYYNDNIQQYQTPE
                     QVRASHILLKTDGKDEAVVRKQAEAVLQQVKSGADFAGLAKKFSEDEASKANGGDLDY
                     FPRGRMVPEFETVAFTLQPGQVSDLVKSQFGFHIIKVVDKRAAVTRSLDEVREQIQNQ
                     LAVQMSDQRVTAQANEFTSRITDAASLERAARELQQTVQESGFFKRDDPVPGLGAAPQ
                     VAAAAFALNGAAAGGPVSSARGPVFFVVSERKDPYVPMLDEVKDRVREDLIRSKAAEL
                     SRQRAAAIASALKPAKDFAAAAKAQGLEAKPTELIARGSALPDVGVSPEVDRVAYGLP
                     VGGVSDPIQTTDGTVIVRVSERDDVTPDELRQAREAFRAELLSERRGRFFAAYMGKAK
                     ERMKIEVNNEVLSRLLAAYRL"
     gene            complement(4923..>5161)
                     /locus_tag="AC16CY_10765"
     CDS             complement(4923..>5161)
                     /locus_tag="AC16CY_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354315.1"
                     /translation="AKERARIPEDEDVQLVVYPARRSLYDALSQEFGGVSGVGLWAMF
                     AGPNERRAMAALSAPVRLFRRGEPLALMPFTLVR"
BASE COUNT          915 a         1696 c         1689 g          861 t
ORIGIN      
        1 catgccgtcc cccgtcccct tgtcgatcgt gatcgtccgg aactcgggac tggcgccgcc
       61 ggcaatcacg gcggcggccg tcgtggacaa cttggtggtc gaccgcaggt cgagcagggc
      121 ctgcagcgac tccgactgct cggccagcgc gcgttgctgc tgcaggccga tcttgagcga
      181 ggcgatctcc tcgcggaggc gcgcgttctc ttcgttgacg cctctgagcg cgacgtagtc
      241 ggtccagccc gagcgaacgg cgccaatcag accgctggca gctcgctgaa cctcggcaaa
      301 gacaccgacc gtggcttcct cgagcactgg aacaccccgg ttggtattca cctgcgcgga
      361 gatcaggagg atgtggccga cgatcacggc caggaagagc cagcccgttc gctgtcggat
      421 atcgagaacg gccatgtgca cacaggcctc aggccacagg gcccaggcct cagtcgatcg
      481 agatcttgcg aagcaggttg aaatccgaga gcatctttcc cgcacccagc accaccgacg
      541 acaggggatc ctcggccatc gccagcggca gccccgtttc ctcgcggagc cgcttgtcga
      601 ggttcttgag gagcgacccg ccgccggtca acacgatgcc ccgatcgacg atgtcggccg
      661 acagttccgg cggcgtgcgc tccagcgcca cgcgcaccgc gtcgacgatc acgttcaccg
      721 tctcggtcag cgcctcgcgg atctcttcgt cgctgatggt gatcgtcttc ggcacgccct
      781 cgatctggtg gcgccccttg atctccatcg tcatcttctc ttcgagcgga aaggcggatc
      841 caatctccat cttgatctgc tccgccgtgc gctcgccgat gaggaggttg tacgccttct
      901 tgatgtacgc gatgatggct tcgtccatct cgtttccagc cacgcggacg gccttgctgt
      961 agacgatgcc ggccagggag atcaccgcga tgtcggtcgt gccgccaccg atgtccacga
     1021 tcatgttgcc cgagggctcg gtgatcggca tcccggcgcc gatggccgcc gccatcgcct
     1081 cttccacgag atgaacctcg ctggccttgg cgcggtacgc gctgtccttc accgcgcgct
     1141 tctccacctg cgtgatctcg gacggcacgc cgatgacgat ccgcggccgg acccagacgt
     1201 tgcgattgtg ggccttcttg atgaagtagg tcagcatctt ctcggtgacc tcgaagtcgg
     1261 cgatgactcc gtccttcatc ggcttgatcg cgacgatgtt gccaggcgtg cggcccagca
     1321 tgtccttggc ttccttgccg accgcctcga cacggccgtt gaccttgttg atcgccacga
     1381 tcgacggctc gttgacgacg atgcccttgc cgcgcgcgta cacgcaggtg ttggcagtcc
     1441 cgaggtcgat ggcgagatcg cttgaaaaca gggaaaaaaa cgagccgagc ggcaacgcta
     1501 ccaatcctcc tgggccacaa ggataccgtc cttccctgca gccttcacgc gatacatggc
     1561 gacgtcggcc gccttgatca ggtcgtcggc cgacttggga ttgtcgcgtt ggagcgtagc
     1621 gaccccgatc gagaccgtct ggtgcaccga cagcccgtca gaggtcagga agcgcgacgc
     1681 gcgcagccgg tcccggatgc gctcggcaac cgacacggcg ccctccgtgt cggtatcggg
     1741 aaggatgagc gagaactcgt cgccgccgaa ccgggccacg acgtctgttt cacgagcgca
     1801 gctacggatg acggccgctg cttccatcag caccttgctg cccgccagat gtccgtgctg
     1861 gtcgttcacc tgcttgaagc tgtccagatc gaggaacagc accgacagcg cgctgccatt
     1921 ccgcacggcg cgcttgaatt ccttgcgcag caccgaattc aggtaccggg aattgtgcaa
     1981 gcgtgtcagg tcgtcggtca ccgacagcag gtcggccttc tggagcgcca gggcgttgtc
     2041 gagagcgata gccgaaggct cgagcagcgt gcgcagcgac atcgcgagcg atggccccat
     2101 cgacggcgcc gatctcgacg gcgtcgaatc gaggcacgcc agcacgccga tgctccggct
     2161 gcgggacatc agcggcagcg cgacggccga gccgagagcg cctggtcctc cccgcgaatc
     2221 cttggccatg tctcccgaga agaactcggt gccgtgacgc atcacccagt tggcggcgga
     2281 ccacagcgac ggcccgaggt tcggcacgag accctcgtcg gccagcacgt tcagatgtcc
     2341 attgacgtca tgcgcgatga cggcccagca ctgggccgga agccagcctt gtccctgacg
     2401 gacgatccac tcggcgacct tccgggggtc gggcgggtag ttggcgttgg cctcgcggat
     2461 agcctccacg aaggcgtccc gtcgatcggc cctggcacgg agcgtccgaa cgcggctgcg
     2521 caggcgcgag gcgaggtcct gccgctgcgt cccgcgcgcc gcacggggcg acgggtcacg
     2581 tcgggcaacg gatctcatgg ctcgctggga ggaggctggc attggcgtcg gtcaaaaggg
     2641 gcgaggcgag ccttaagtcc ttctaaatgt gagaaataat accatgaccg tggtgacggt
     2701 cctgcggcac ggtctgtagt tttcatcgga ttcggaggcc tcaaactgta ggattggtcg
     2761 gccccccgcc ggaccgaact tcaacagggc tccggttggg cttcccgccg gctgccgctt
     2821 cggcaactgg cggtaaatga ttgccgtagt gtcatttcct gacgtacgat cctcgtttcg
     2881 aggactgctc ccctatgacg atgctcgacc gcatgcggcg gcacaagaac tggctcaaat
     2941 ggagcctggc cgtcgtcgtg ctgaccttcg tgctccttta catccccgac ttcgtccaga
     3001 ccactccgac cggtacgggc gccggaccgc gcgaggtcat cgccgaggtg gacggccgga
     3061 cgctgaccgc gggcgacttc cagcagcggt atctgacgca gctgcaggcc taccggaacc
     3121 agttgggtgg caacgtgagc gaccagctgc tccggcagct cgggatcgat cagcagatcc
     3181 tgtcacagat gatcgacgaa caggtcgcgt tgatcgaggc cgatcggaat ggcatctcgg
     3241 tgagcgacga cgagctggcg cagcagatct tcgcaattcc cggtctgcag gagaacggac
     3301 gtttcatcgg cgaggcgcgc tacgagcagc tgctcttgaa ccaggtgccg ccgatgacca
     3361 aggcgcagtt cgaagagagc ctgcgccgca gcatggtcct cgacaagctc cgcggagcgc
     3421 tgaccgactg gatggccgtt tcggaagccg acgtcgagcg cgagtaccac cagcgcaacg
     3481 agaaggtgaa gatgcaggtc gtcgcgctga cagcggaccg tttccgcagc ggggtgtcgg
     3541 tgaccgatgc ggatgtggcc gcccacttcg aggcgcacaa ggcagagtac cggatcggag
     3601 agcagcgcaa gatcaagtac ctggtgctgg atcgcgatca ggcccgcttg aaggtctccg
     3661 tcccggcgac cgacatccag cgctactaca acgacaacat ccagcagtac cagacgcccg
     3721 agcaggtgcg ggcgagccac atcctgctga agacggacgg caaggacgag gccgtcgtcc
     3781 ggaagcaggc cgaagccgtc ctgcagcagg tcaagagcgg cgccgacttc gcggggcttg
     3841 caaagaagtt ctccgaggac gaggcgtcga aagccaacgg cggagacctc gactacttcc
     3901 ctcgcggccg catggtgccg gagttcgaga cggtggcgtt cacgctccag ccaggccagg
     3961 tgagcgacct ggtcaagtcg cagttcggat tccacatcat caaggtggta gacaagaggg
     4021 cggccgtcac tcgttcgctc gacgaagtgc gtgagcagat ccagaaccag ctcgccgtgc
     4081 agatgtcgga tcagcgggtc accgcccagg ccaacgagtt cacgtcgcgg atcacggacg
     4141 ccgccagcct ggagagggcc gcgcgcgagc tccagcagac cgtgcaggaa tcagggttct
     4201 tcaagcgaga cgatcccgtg cccggtctcg gagccgcccc ccaggtcgcg gcggcggcct
     4261 ttgcgctgaa cggcgccgcg gcgggcgggc cggtcagctc tgcccgcggt cccgtgttct
     4321 tcgtcgtgtc cgaacgcaag gacccgtacg tgccgatgct cgacgaggtg aaagatcggg
     4381 tgcgtgagga cctgattcgc tcgaaggcag ccgaactgag ccggcagcgg gcagcggcca
     4441 ttgcctccgc gctgaagccg gctaaggatt tcgcggcggc ggccaaggcg cagggcctgg
     4501 aggccaagcc gaccgaactg atcgcccgcg ggtccgcgct gcccgacgtc ggggtcagcc
     4561 ccgaggtcga tcgcgtggcg tatgggctcc ccgtgggcgg cgtcagcgac ccgatccaga
     4621 cgaccgacgg caccgtcatc gttcgcgtca gcgaacgcga cgacgtcacg ccggacgaac
     4681 ttcggcaggc acgcgaggcg ttccgcgcgg aactgctctc ggaacgtcga ggccgcttct
     4741 ttgccgccta catgggaaaa gccaaggagc ggatgaagat cgaggtgaac aacgaggtgc
     4801 tcagccggct cttggcagcg tatcgcctct gagtgactcg ccgcgcacgc tgaagcgtgc
     4861 gctctacgac cgaacggcgc tgcgatcgta gagcggcggc ttcagccgcc gcgtgacggc
     4921 cttcatctca ccaacgtgaa tggcatgagc gccagcggct ctccgcgccg gaagagacgc
     4981 acgggcgccg acagcgcagc catcgcgcgg cgctcgttgg ggcctgcaaa cattgcccac
     5041 aagcccacgc cgctgacgcc gccgaactcc tgagaaagcg cgtcgtacag gcttcggcgc
     5101 gccggataga cgacgagctg cacgtcctcg tcctccggaa tgcgggcgcg ctccttggcg
     5161 a
//
LOCUS       DBNGSH010000460         3767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2589846, whole genome shotgun sequence.
ACCESSION   DBNGSH010000460 DBNGSH010000000
VERSION     DBNGSH010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2589846"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="AC16CY_10770"
     CDS             <1..1018
                     /locus_tag="AC16CY_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3354316.1"
                     /translation="RRIELIVEDDEAIPAVGLTKARKLVERDEVHVMAGALLSSTGYA
                     LAPYIDSMRIPMVYPVVSADDLTQRRRSQWIVRTGWTGSQPNHAFGEYAYRALGLRSV
                     VTIALDYAFGWESVGGFERTFVEQGGRVTRKIWVPVSVHDFAPYLAQVSRDVDAVYAL
                     VLGRSALQFMRQYEEFGLKGRVPLIGGGTTTDEHVLPFMGDEAIGTVTALHYSAALDT
                     SANRVFAEAYRARFKKVPSYYSESMYTGGKWLVAAIDAVGGNVENRAALVEALRRVKP
                     TGLPRGPVELDTYGNPIENVYIRRVERVNGELQNTVIETFPKVSQFWKYNPAEYLRSP
                     LYAR"
     gene            1737..2432
                     /locus_tag="AC16CY_10775"
     CDS             1737..2432
                     /locus_tag="AC16CY_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354317.1"
                     /translation="MKGYIKALQYFREAYSILDYHCPRMQSRSEWNMAARAAYEAMEK
                     VVKAVELLADVHLTTQHDVTLHQQVLDRLPLVAWRVDDDPMTSISLCAVKKHSPDLHV
                     TLSKVSRGMFTSLASGRWDGSSPLSMTMSGSTIQVLSGTTAVFGVTDTTAGVSLEGHW
                     IQVLISQPQVELLNDFLKSVSKGRNVSAYYGKVFDAQSAEAVRGNGMSLFVPVKRRVG
                     HRIHGSPLFDGAA"
     gene            complement(2509..2778)
                     /locus_tag="AC16CY_10780"
     CDS             complement(2509..2778)
                     /locus_tag="AC16CY_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParE family toxin-like protein"
                     /protein_id="HIR3354318.1"
                     /translation="MTSLTTPRFWAAYRELPIEVRDAARKAYALFRENPQHPSLQFKK
                     VHEREPVHSVRVTIAYRAVGLLENDTITWFWIGSHADYDRLLKSL"
     gene            complement(2775..2993)
                     /locus_tag="AC16CY_10785"
     CDS             complement(2775..2993)
                     /locus_tag="AC16CY_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354319.1"
                     /translation="MTRMLEAAIAKLATLPPDEQDRVGRWLLDELRDEDHWARQFAVS
                     QDILSKLAAEARADLAAGRTTPLDPEKL"
     gene            3013..3156
                     /locus_tag="AC16CY_10790"
     CDS             3013..3156
                     /locus_tag="AC16CY_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354320.1"
                     /translation="MLVTRFQRLRWQDGGELQDTVIETFLKVSQFWTYDPENYLKEPL
                     YVR"
     gene            3169..>3767
                     /locus_tag="AC16CY_10795"
     CDS             3169..>3767
                     /locus_tag="AC16CY_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3354321.1"
                     /translation="MPETAAAACLDLRGVSRHFGGLRAVDDVSLSVPPGARHAVIGPN
                     GAGKTTLFNVISGELLPTSGAIYLSGADITRLAPHRRAARGISRTFQITRLFPDLTVM
                     ENVLLACAAQDRRRFRMLRPVPSYSDLVERATDLLERFALSPLSQERAKHIAYGDQRR
                     LEVALSLAGRPRLLLLDEPMAGLSTPERAAMRELLEDLDP"
BASE COUNT          681 a         1162 c         1234 g          690 t
ORIGIN      
        1 ccggcgaatc gagttgatcg tcgaggacga cgaggcgatt cccgcagtcg gcctcacgaa
       61 agcgaggaag ctggtcgagc gggacgaagt gcacgtcatg gccggcgcgc tgctgtcctc
      121 caccggatac gccctggcgc cctacatcga ctcgatgcgg atcccgatgg tgtaccccgt
      181 ggtgtcggca gacgacctga cgcagcggcg gcgcagccaa tggatcgtgc gcacggggtg
      241 gaccggcagc cagccgaatc atgccttcgg cgaatacgcc tatcgggcgc tcggacttcg
      301 gagcgtggtg acgatcgcgc tggactacgc gttcggatgg gaatcagtcg gcggtttcga
      361 gcggacgttc gtggagcagg gcggccgcgt cacacggaag atctgggtgc cggtgtcggt
      421 gcacgacttc gcgccgtacc tggcgcaggt ctcgagggac gtggacgcgg tgtacgcgct
      481 ggtgctcgga aggtcggcgc tccagttcat gcggcagtac gaagagttcg ggctgaaggg
      541 acgggtgccg ttgattggcg gcggcacgac gacagacgag cacgtcctgc ccttcatggg
      601 tgacgaggcg atcggcacgg tgaccgcgct gcactacagc gcggcgttgg acacttcggc
      661 caacagggtc ttcgcggagg cctaccgcgc gcgcttcaag aaggtgccga gctactactc
      721 ggagagcatg tacaccggcg gcaagtggct cgttgcggcc atcgacgccg tcggcggcaa
      781 cgtggaaaac cgcgcggcgc tcgtcgaggc gctccggcgg gtgaaaccga ccggcctgcc
      841 gcgcgggcca gtagaactcg acacttacgg caatcccatc gagaacgtct acatccgccg
      901 ggtcgagcgc gtgaacggcg agctgcagaa caccgtcatc gagacgtttc cgaaggtgtc
      961 acaattttgg aagtacaacc ccgccgagta tctgcgctca ccgctgtacg cgagatgagt
     1021 gcacgctcga ggttctatct caccgccctc atcgcgtcgc gacggagtca ggagccccgt
     1081 cgatcgggtg tggatctcat tgtgagcaat tgacgacaat ttccgcgggc cacccgtgtc
     1141 tggtcagccg ccattcgacc cggcgctccg gtcggacgtc gtggaaaacc aggccctcca
     1201 catggtttcg gcgacccgcg cagctccgcg agctgcgcgg ccctctcgtt tcgggcccgg
     1261 tttaccccgc tctcgcgtgt cacgacgccg acagtcgcgc ctcccgaacg gcggctgacc
     1321 agacacgggt ggcccgcgag aaccggttcc ctgtgatcag gatgagagtc acaccccgtc
     1381 gatccatgct gacggacggc tgctgataac ggcgtcgaat tagcccgcgt ggcgcgcggg
     1441 ttatttggga tcagcgcgac gcgccgcatc ggcaacacgc ttcagcgcgc tcgacacttg
     1501 ccgccgccgg caccgcaggt tggcccgcct cataacacgc ggggcccgcg cgtattcctg
     1561 ccggccgcgc gttcacacgg cgaggagagc gggcctgtcc cgcgtgttat catcccgcgt
     1621 tcacaggctt tagtatctgg gcggtgtgcg tgcccagttg tacgcggcga gcgagcgctc
     1681 cggtctactc cagagactgt tagaccggcc gttgctcgcc atccaacgca agaatcatga
     1741 aagggtacat caaagcctta cagtacttcc gcgaggctta cagcatactg gactaccatt
     1801 gcccaagaat gcagtcgcgc agtgagtgga acatggcggc acgcgcagct tacgaagcga
     1861 tggaaaaggt ggtgaaggct gtcgagcttc tcgctgatgt tcatctgaca acccaacatg
     1921 atgtcacact gcatcagcaa gtattggatc gactgccgtt ggtcgcgtgg cgtgtcgacg
     1981 atgatccgat gacgagcatc agcctgtgcg ctgtcaagaa acacagtccg gacttgcacg
     2041 tcactctgag caaggtgtct cggggaatgt ttaccagcct ggcaagcgga cggtgggacg
     2101 gctcgagccc gctcagcatg acaatgagcg gctcgaccat ccaggtattg tctggtacga
     2161 cggcggtctt tggggtgact gatacgactg ctggagtcag ccttgaaggc cattggatcc
     2221 aagtactgat cagccagccc caagtggaac tgttgaacga ctttctcaag tctgtgtcca
     2281 aaggccgaaa tgtcagcgct tactatggca aagtgtttga cgctcagtcg gccgaggccg
     2341 ttcgcggcaa tggaatgagc ctcttcgtac ccgttaaacg tcgggttggc catcggatcc
     2401 acggttcacc gctgttcgat ggggcagcct aacaactccg cttgcaaccc acgacgcttg
     2461 gtgcgattgg gaagcgccgc ggctgaagcg gatcgttggg ccaacgaact acagactttt
     2521 gagcagacgg tcgtagtcag cgtggcttcc gatccagaac caggtgatcg tgtcgttctc
     2581 gagcaggccg acggcacggt acgcgatggt gacgcgcacg gagtgcaccg gttcccgttc
     2641 gtgaaccttc ttgaattgga ggctggggtg ctgcgggttc tcgcgaaaga gagcgtaggc
     2701 tttccgagcg gcatcccgaa cttcgatggg caactcacgg tacgccgccc agaagcgcgg
     2761 cgtcgtgagc gacgtcacaa cttctcggga tcgagtggtg tcgttcggcc ggccgcgaga
     2821 tcggctctgg cctccgccgc gagcttgctc aggatgtcct gcgacacggc aaactgccga
     2881 gcccagtggt cctcatctcg gagttcgtcg agcaaccagc gtccgacgcg gtcttgttcg
     2941 tctggaggca gtgtcgccag cttggcaatg gccgcctcca gcattcgagt cataggaaaa
     3001 gtataacgga caatgctcgt tactcgcttc cagcgacttc gatggcagga cggcggcgag
     3061 ctgcaggaca ccgtcatcga gacgttcctc aaggtttcac agttctggac gtacgacccc
     3121 gagaactact tgaaagaacc gctgtatgtg aggtaagtac gcgcggccat gcctgaaaca
     3181 gcggccgctg cgtgcctgga tctccgcgga gtctcgcgcc acttcggcgg cctgagggcc
     3241 gtcgacgacg tgagcctgtc agtgccgccg ggggcgcggc acgccgtcat cggtccaaac
     3301 ggcgccggca agacaacgct gttcaacgtc atcagcggcg agctgctgcc gacgagcggc
     3361 gccatctacc tctccggtgc ggatatcacg aggctggcgc ctcatcggcg cgcggcgcgc
     3421 ggcatctcgc gcacgtttca gatcacccgg ctctttcccg atctcacggt gatggagaac
     3481 gtcctcctgg cgtgcgccgc acaggaccgg cggcggttca ggatgctgcg gccggtgccc
     3541 tcgtattccg atctcgtgga gcgggccacg gacctgctcg agcggtttgc gctctcgccg
     3601 ctttcgcagg agcgggcgaa gcacatcgcc tacggcgacc agcgccggct cgaggtggct
     3661 ctctcgctgg ccgggcggcc gcggctgctg ctgctcgacg aaccgatggc ggggctgtcc
     3721 acgcctgaac gcgcggcgat gcgggagctt ctcgaggacc tcgatcc
//
LOCUS       DBNGSH010000461         2426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3948023, whole genome shotgun sequence.
ACCESSION   DBNGSH010000461 DBNGSH010000000
VERSION     DBNGSH010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3948023"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="AC16CY_10800"
     CDS             complement(<1..309)
                     /locus_tag="AC16CY_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354322.1"
                     /translation="MAIGRFAFVVVVASIGLGTLSAGRADAQTSPARFVTVDQGADEF
                     AADEVLARRLDTPLIGKPERLSYEAVINALLDDARRDTPLARAGGVVARVTPYAFVVA
                     E"
     gene            551..1231
                     /locus_tag="AC16CY_10805"
     CDS             551..1231
                     /locus_tag="AC16CY_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3354323.1"
                     /translation="MQTGHRTWINMLSVAVVLIGGAQVGSAGASSVSKEASHGSAVEH
                     PAAVSAKTLRLREDMRKLWSDHVIWTREYIVSAIDGSPDANAAATRLLKNQDDIGGAV
                     AAFYGKDAGDTLTRLLKEHILIAVDLIAAAKAGDTAKYSATDQRWTRNGEEIADFLSQ
                     ANPNWPRAALADMMAAHLATTTKEVVARMNKKWDEDTAAFDEAYAHILHMSDALSEGI
                     VKQFPHKF"
     gene            1270..>2426
                     /locus_tag="AC16CY_10810"
     CDS             1270..>2426
                     /locus_tag="AC16CY_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011045570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3354324.1"
                     /translation="MKLIGTASTVFVLLLSSIVFGPGQSTTAQQRDRDGQAIFRYDTF
                     GDEQLWTEVLRMQEPIATVDPATALAVGLKVDVDALPPRIVGALRAGQVDLTSPAVTT
                     ELLRLNAVVGVKGTVNETGQLTSVGVTCALCHSTVDNSFSPGIGRRLDGWANLDLNVG
                     AIVALSPALPDTLKAEFRTWGPGKYDPRHHAFDGANLVPLNGPSLPIVIPPIYGLKGV
                     GFETYTADGPISYWNSYVGVGQMGGHGSFSDPRIGLFITQTPDLVTPKLAALLDYQLS
                     LRVPDPPAGSFDKEAAKRGKQLFRHEAGCATCHRSPNYTDVLSGPVRTVPFLHDPAEV
                     GTDPAYAARSATGQYRTTPLRGLWQHAPYFHDGSAPDLLAVVNHYNRLFAL"
BASE COUNT          468 a          790 c          758 g          410 t
ORIGIN      
        1 ttcggcgacc acgaatgcat aaggcgtcac ccgcgccacg acgccgcccg cccgcgccag
       61 cggcgtgtcg cggcgcgcgt cgtcgagcag cgcgttgatc acggcctcgt aggacagccg
      121 ctccggcttc ccgatcagcg gcgtgtcgag gcggcgggcc agaacctcgt ccgcggcgaa
      181 ctcgtcggcg ccctgatcga cggtcacgaa gcgggccgga gacgtctgtg cgtcggcgcg
      241 gccggcgctc agcgttccga gcccgattga cgccacgacg acgacaaacg cgaatcgccc
      301 gatggccata ttcaatggga tcaacgatgg cggcccgaga tctgaacgcg ctgtcgcggc
      361 ctcggcgctc ttacctcatc ctttgagcga atcctgcaca ggaattccct gcgaaatcat
      421 gccgcgggaa cgttttcatc ttgtctgact ctgtacggtc aggagcgaga tgacgccggc
      481 ggcgcaagga tactcgcaat cgcgagactc gttgccggtg gctccagcat cgcgcgaagg
      541 agaggactgc atgcagactg gacaccggac gtggatcaac atgttgagcg ttgccgtcgt
      601 gctgatcggt ggcgctcagg tcggcagtgc gggcgcatcg tctgtctcga aagaggcgag
      661 tcacggatcg gcagtcgagc accccgccgc cgtatccgca aaaacgctac gactccgcga
      721 ggacatgagg aagttgtggt cggatcacgt catctggacg cgagagtaca ttgtgtcggc
      781 aatcgacggc tcgcccgatg ccaacgccgc cgcaactcga ctcctcaaga atcaagacga
      841 catcggcgga gcggtggcgg cgttctacgg caaagacgcg ggcgatacgc tcacgcgctt
      901 gctcaaagag cacatcctga tcgccgtcga tctgattgcg gcggccaaag ccggcgacac
      961 ggccaagtac tccgcgaccg accagcggtg gacgaggaac ggcgaggaga tcgcagactt
     1021 cctcagccag gcgaacccga actggcccag ggccgcactg gccgacatga tggccgcgca
     1081 cctggccacc accaccaagg aagtcgtggc gcggatgaac aagaaatggg atgaagatac
     1141 cgcggccttc gacgaagcct acgcccacat cctgcacatg tccgacgcgc tgtccgaggg
     1201 gatcgtcaag cagttccctc acaagttctg agacgccgcg ccatcctcgc gtgatgcaag
     1261 ggagccgcca tgaaactcat cgggaccgcg tcaaccgtat tcgttcttct cttgtcgtcc
     1321 atcgtcttcg gacctggtca atcgacaacc gcgcagcagc gtgatcgaga cgggcaggcc
     1381 atcttccgct acgacacgtt cggcgacgaa cagctgtgga ccgaggtgct ccgcatgcag
     1441 gagcccatcg cgaccgtcga tcccgccacg gccctcgcgg tgggcctcaa ggtggatgtg
     1501 gatgccctcc cgccgcgcat cgtcggcgca ctccgagcgg gacaggtcga tctcacgagt
     1561 cccgccgtga ccacggaatt gctacggctg aatgcggtcg tcggtgtcaa aggtaccgtg
     1621 aacgagacgg gccagttgac cagcgtcggc gttacgtgcg cgctgtgcca ctcgacggtc
     1681 gacaactcct tctcgcctgg gatcggaagg aggctggatg gatgggcgaa tctggacttg
     1741 aatgtggggg cgattgtcgc gctgtcgccg gcgttgcccg atacactcaa ggccgagttc
     1801 cgcacgtggg gccccggcaa atacgatccg cgacatcacg cgttcgatgg cgcgaacctc
     1861 gtcccgctga acggtccatc gctgccgatc gtgatcccgc cgatctacgg attgaaaggc
     1921 gtcggcttcg aaacctacac cgccgacggg ccgatctcgt actggaacag ctacgtggga
     1981 gtcggacaaa tgggcggtca cggcagcttc agtgatccac gcatcggtct tttcatcaca
     2041 cagacaccgg atctggtcac tccgaagctc gcggcgctgc tggactatca gctgagtctg
     2101 cgcgtccccg atccgccagc cggctcgttc gataaagaag ccgcgaagcg cggcaagcaa
     2161 ctctttcgtc acgaggcggg atgcgccacg tgccatcggt cgccgaacta cactgacgtc
     2221 ctgagcggtc ccgttcgaac ggtgccgttc ctgcacgatc ccgccgaggt cggcaccgat
     2281 ccggcttatg ccgccaggtc ggcaaccgga caataccgga ccacgccatt gcgcggactc
     2341 tggcagcatg cgccgtactt tcatgatggc agtgcccccg acctcctcgc ggtggtgaat
     2401 cactacaacc gattgttcgc gctcga
//
LOCUS       DBNGSH010000462         2450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_212690, whole genome shotgun sequence.
ACCESSION   DBNGSH010000462 DBNGSH010000000
VERSION     DBNGSH010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_212690"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1037
                     /locus_tag="AC16CY_10815"
     CDS             72..1037
                     /locus_tag="AC16CY_10815"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3354325.1"
                     /translation="MRAVILSARAGGHTTELLRALTERGHSGDVRPYEGLVARVGNVP
                     VEAGLTWPEQSRRQTCLTSDHTSILDADAVFARIIPGGSLEQIIYRVDALHWIEDRGV
                     LVVNTPRAIERCVDKFYTTTLLHDAGLPTPETVVCERLDEAVAAVEAMGDVVIKPIFG
                     SLGHGMIRVSEPELARRVLRPLDQVGSVFYVQRAIDHGGRDVRAFVVGGRVLGAIERR
                     APEGEWRTNVAIGGTARAIDLTPEMEQMAQRAAAVVGAASAGVDLLPSRDGGVFVLEV
                     NGIPGWQGLQEATGLDVATAIVAHAERRLESRRSSSMRAEEPLPA"
     gene            1034..1975
                     /locus_tag="AC16CY_10820"
     CDS             1034..1975
                     /locus_tag="AC16CY_10820"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HIR3354326.1"
                     /translation="MSVYRDLAAALLAVDRAHAPVNTGLAAQLACLLEVSAPKPGNVS
                     PGRNFADLRYEDFLASAVAIGEPLVGAGSRGVGETVRLCIESTSRWTRSNTNLGIVLL
                     FAPIAKAKWGRESFLDVPREMTPDPISLRDAVRAVLESTTVADARETYAAIRLAGPGG
                     LGQVESQDVSAEPTMTLVEVMRLAAHRDTIAAEYATGFAITFETAVPALERARRDGLP
                     WDDAVVETFLTVLASVPDTHIARRSGAGRSIEVTERAQRVVAAGGVRTPEGRRAIEEM
                     DMALRDERNSGNPGTTADLTAAAIFVVLLGGGFHAAR"
     gene            1962..>2450
                     /locus_tag="AC16CY_10825"
     CDS             1962..>2450
                     /locus_tag="AC16CY_10825"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR3354327.1"
                     /translation="MQRGEFRVSVTKDHLVFSSAHFITFAGHRCEGLHGHNYRARVTI
                     EGALNQESWFVFDFVELKKIMKKLCDEIDHLVLLPTRSERIQVAESGETVSVSVDGKP
                     RYLFPRKDCALLPVPNTTVEMLAEMLTDRLRTELATAGAQGMTAIEMEVEENFGQSAV
                     YRV"
BASE COUNT          379 a          806 c          889 g          376 t
ORIGIN      
        1 gcggacgaac gttccacgca ggcggactca acgccgacgt gctgcgcgct tcgctgtacg
       61 ggagctgacg gatgcgcgcc gtgatcctca gcgcacgcgc gggcgggcat acgaccgagc
      121 tcctgcgcgc cctgaccgag cgagggcaca gcggtgacgt gcgcccctac gaaggcctgg
      181 tcgcgagggt tggcaacgtt ccggtagagg cgggtcttac ctggcctgaa caaagtcgaa
      241 ggcagacctg cctgaccagc gaccacacct ccattctcga cgccgacgcc gtgtttgccc
      301 ggatcattcc cggcggatcg ctggagcaga tcatctaccg cgtggacgcg ctgcactgga
      361 tcgaggatcg cggcgtcctc gtcgtgaaca caccgcgcgc aatcgagcgg tgcgtggaca
      421 agttctacac gaccacgctg ctgcacgacg ccgggctgcc cacgcccgag accgtcgtct
      481 gcgagcgctt ggacgaggcc gtggcggcgg tcgaggcgat gggcgacgtg gtgatcaagc
      541 cgatattcgg gtcgctcggg cacgggatga tccgcgtgag cgagccggag ctggcgcgtc
      601 gcgtgctgcg cccgctcgat caggtcggct ccgtgttcta cgtccagcgc gcgatcgatc
      661 acggcggccg cgacgtgcgc gcgttcgtcg tcggcgggcg ggtgctcggt gcgatcgagc
      721 gccgcgcgcc cgaaggggaa tggcgcacca acgtcgccat cggcggaacc gcgcgcgcca
      781 tcgatctgac tcccgagatg gagcagatgg cgcagcgggc cgcggccgtg gtgggcgccg
      841 cctccgccgg cgtggacctg ctgccgtcgc gcgacggggg cgtcttcgtc ctcgaggtca
      901 acggcatccc gggatggcag ggcctgcagg aggccaccgg cctcgacgtc gcgacggcca
      961 tcgtcgcgca cgccgagcgg cgcctcgagt cgaggcgctc ctcgtcgatg cgcgcggagg
     1021 agccgctgcc ggcatgagcg tctatcgcga cctggcggcc gccctgctcg ccgtggaccg
     1081 tgcgcacgcg ccggtgaaca ccggcctcgc ggcgcagctc gcctgcctgc tcgaagtcag
     1141 cgcgccgaag cccgggaacg tgtcacccgg ccgcaacttc gccgacctcc ggtacgagga
     1201 ttttctggcg agcgccgtgg ccatcggcga gccgctcgtc ggcgcgggat cgcgcggcgt
     1261 cggcgagacg gtccgactgt gcatcgagtc cacgtcccgc tggacgcgtt cgaacacgaa
     1321 ccttggcatc gtgctgctgt tcgcgccgat tgcaaaagcg aaatggggtc gggagtcatt
     1381 tcttgacgtc ccacgagaaa tgactcccga ccccatttcc ctccgcgacg ccgttcgggc
     1441 ggttctcgag tccaccacgg tcgctgatgc gcgcgagacg tatgcggcca tcaggctggc
     1501 aggcccggga gggctggggc aggtggaatc gcaggacgtg tccgcggagc cgacgatgac
     1561 gctcgtcgag gtgatgcggc tggccgcgca tcgcgacacg atcgcggccg agtatgcgac
     1621 cggcttcgcg attacgttcg agaccgccgt gccggcgctc gagcgtgccc gccgcgacgg
     1681 cctgccctgg gacgatgccg tcgtcgagac gttcctgacg gtgctggcgt cggtgcccga
     1741 cacgcacatc gcccgccgga gcggcgccgg acgatcgatc gaggtgaccg agcgcgcgca
     1801 gcgcgtcgtc gcggccggag gcgtgcgcac gcccgagggg cggcgggcga tcgaagagat
     1861 ggacatggct ctccgcgacg agcggaacag cggcaacccg gggacgaccg ccgacctgac
     1921 ggcggccgcg atcttcgtcg tgctgctcgg cggaggattc catgcagcgc ggtgagttcc
     1981 gcgtgtcggt caccaaggat cacctggtgt tttcgtcggc gcacttcatc accttcgccg
     2041 ggcaccgctg cgagggtctg cacggacaca attaccgtgc gcgcgtgacg attgaaggcg
     2101 ccctcaacca ggagagctgg ttcgtgttcg acttcgtcga gctcaagaag atcatgaaga
     2161 agctgtgcga cgagatcgat cacctcgtgc tgctgccgac caggagcgag cgcattcagg
     2221 tggcggagag cggcgagacg gtgtcggtgt cggttgacgg gaagccgcgc tacctgttcc
     2281 cgcgcaagga ttgcgcgctg ctgccggtcc cgaacacgac ggtcgaaatg ctggccgaga
     2341 tgctgaccga tcgcctgcgg accgagctcg ccacggcggg cgcacaaggg atgacggcca
     2401 tcgagatgga ggtcgaggag aatttcgggc agtcggcggt gtatcgtgtg
//
LOCUS       DBNGSH010000463         2798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1910628, whole genome shotgun sequence.
ACCESSION   DBNGSH010000463 DBNGSH010000000
VERSION     DBNGSH010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1910628"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="AC16CY_10830"
     CDS             complement(<1..1321)
                     /locus_tag="AC16CY_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR3354328.1"
                     /translation="MIIRTTLGSMCVVLAVSAVPAFAQAFANASTSLANYTAADSTPR
                     KACESLASFKGEGIVSISARVVPAASGTPQHCRVTGMIAPEVAFEVSLPDRWNRRFYM
                     TGNGGLAGDAVDGPTNADRTAGLSNGFVHARTNTGHDARKEPSGSFILSNPQKALDYA
                     YRAVHVTADTAKKIAADYYAQPIQFSYWNSCSNGGRQGLLEAQRYPDDFDGIVANAPW
                     VDQTGFAVGAIWNQKALTEAPVSPAKMVLVGQKVMDKCDAADGLKDGLIDDPRACRFS
                     AARDVPACSAGVDAEDCLTAAQAAAIDKIYAGPTSNGKPFIAGFMPGSEAVTTSANGT
                     SNSGWVGAIVPAQPGAKPADFNLAEGVVRYLILEPPQPEYDSLTFDYNRAAALVARWS
                     KLADAKEADLSGFRKSGGKLLMTYGWADQILQPMMGVNYYEAVVAKNR"
     gene            complement(1366..2646)
                     /locus_tag="AC16CY_10835"
     CDS             complement(1366..2646)
                     /locus_tag="AC16CY_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3354329.1"
                     /translation="MSLPDKPDLDWLRKQAKRRLAEMREADPAAQLSDAQLDLARAHG
                     FSSWRALKAHIDGLTVEGKLFEAARVGDVGTLRTLLDAHPEKLHARKQPYEGSLLHHA
                     ARHLAAVDLLLERGLDPNAREKGDNTYAMHWAASAGRLDVVRRLADAGGDVIGEGDDP
                     ELGVIGWATCWNDCDDDDHRAVADFLVSRGATHHIFSAIAMNLGDEVRRIVAADASAL
                     NKRQSRNENHRTPLHFAVAMNRQEMISLLLDLGADPLAVDGSGQPVAAYVTSPGTDRR
                     VMEKIVEMTSAEFVSASRGHRAPRGSPMDLAALVSLGDWDAAARLLRENASLIEPGGG
                     VLHLMAKRNDATALRWLLDHGADVNGRWGDADVTPLHLAASQGHVEAARVLLTAGADS
                     TIRDSQHDSDALGWASFFQKPDIVRLIVEQRGKA"
BASE COUNT          434 a          947 c          914 g          503 t
ORIGIN      
        1 tcctgttctt cgccacaacg gcttcgtagt agttcacgcc catcatcggc tgaagaatct
       61 ggtccgccca cccataggtc atgaggagct tgccgccgct cttgcgaaac ccggacaggt
      121 cggcctcttt cgcatcggcc agcttgctcc agcgcgcgac gagcgcggcg gcgcggttgt
      181 aatcgaacgt cagcgagtcg tactccggct gcggcggctc gaggatcaga tagcgcacca
      241 cgccttcggc caggttgaag tcggcgggct tcgcgccggg ctgcgccggc acgattgcgc
      301 ccacccatcc gctgttcgag gtgccattcg ccgacgtcgt caccgcctcg ctgcccggca
      361 tgaaaccggc aatgaacggt ttgccgttgc tggtcggacc ggcgtagatc ttgtcgatcg
      421 ccgctgcctg ggccgcggtc aggcagtcct ccgcgtcaac accggcgctg catgccggta
      481 cgtcgcgcgc agcgctgaag cggcacgcgc gcgggtcgtc aatcaatcca tccttgaggc
      541 cgtcggccgc gtcgcatttg tccatcacct tctgcccgac cagcaccatc ttcgcgggtg
      601 agaccggcgc ctccgtcagc gccttctgat tccagatcgc tccgacggcg aacccggttt
      661 gatcgaccca gggcgcgttg gccacgatgc cgtcgaaatc gtcggggtag cgctgcgctt
      721 ccagcagtcc ctgacggccg ccgttcgaac aggagttcca gtacgagaac tgaatcggct
      781 gcgcgtagta gtcggccgcg atcttcttcg ccgtgtcggc cgtgacgtgc acggcacgat
      841 acgcgtagtc gagggctttc tgcgggttgc tgaggatgaa cgacccgctc ggctcctttc
      901 gcgcgtcgtg gcctgtattg gtccgcgcgt gcacgaagcc gttgctcagt ccggcggtcc
      961 tgtcggcgtt ggtcggaccg tccacggcat cgcccgcgag gcccccgttg ccggtcatgt
     1021 agaagcggcg gttccagcga tccggcaggc tcacctcgaa cgcgacctcg ggcgcaatca
     1081 tgccggtcac ccggcagtgc tgcggtgtac ccgacgcggc cggtacgacg cgggcactga
     1141 tcgacacgat cccctcgcct ttgaacgacg ccaagctttc acacgccttt cgcggcgtgg
     1201 aatccgcggc ggtgtaattg gcgagcgatg tgctggcatt cgcaaacgcc tgcgcgaatg
     1261 ccgggacggc ggagaccgcc aggacgacac acatggagcc gagagtcgtt cgaatgatca
     1321 cgtctggttc tccttcgctc gggcagtata cggcttccag ccgtctcacg ccttcccgcg
     1381 ctgctcgacg atgagccgga cgatgtccgg cttctggaag aaactggccc atccgagcgc
     1441 gtcgctgtcg tgttggctgt cgcggatggt cgaatcggcg ccggccgtca gcaggacgcg
     1501 cgcggcctcc acgtgtcctt gagaggctgc cagatgcagc ggcgtcacgt cggcgtcccc
     1561 ccatcgtccg ttcacgtcgg cgccgtggtc gagcagccat ctgagggccg tcgcgtcgtt
     1621 gcgtttggcc atcaggtgca gcacaccgcc gcccggctcg atcagactgg cattctcgcg
     1681 aaggagccgc gcagccgcgt cccagtcgcc gagcgacacg agagcggcga gatccatcgg
     1741 actgccacgg ggtgcgcgat gtccacgaga cgcgctgacg aactccgccg acgtcatctc
     1801 gacgatcttc tccatcacgc gccgatcggt cccgggcgag gtcacgtacg ccgcgaccgg
     1861 ctggccagag ccgtcaaccg ccagaggatc ggcgccaaga tcgagcagaa gcgaaatcat
     1921 ctcctgccgg ttcatcgcca cggcaaagtg gaggggcgtt cggtgattct cgttccggct
     1981 ctgtcgctta ttgagggccg acgcgtcggc ggcgacaatc cggcgcacct cgtccccgag
     2041 gttcatcgcg atcgcggaaa agatgtgatg cgtcgcgcca cggctcacta ggaagtcggc
     2101 gacggcgcga tgatcgtcgt cgtcgcagtc gttccaacag gtcgcccagc cgatcacgcc
     2161 gagctcgggg tcgtcgccct ccccgatcac gtcgcccccg gcgtcggcca gacgccgcac
     2221 gacgtcgaga cggcccgcgg aggcggccca gtgcatcgcg taggtgttgt cgcccttctc
     2281 acgggcgttt gggtccaggc cgcgttcgag cagcaggtct accgccgcga ggtgtcgagc
     2341 cgcgtggtgc aggagcgacc cttcgtacgg ctgcttccgc gcatggagct tctctggatg
     2401 cgcgtcgagg agcgtgcgca gcgtgccgac gtcgcccacg cgcgccgctt cgaacagctt
     2461 tccctcgacg gtcaacccat cgatgtgggc tttgagcgcc cgccagctcg aaaacccgtg
     2521 cgccctggcc agatcgagct gggcgtctga gagctgcgcg gcggggtcgg cctctcgcat
     2581 ctccgcgagg cggcgtttcg cctgcttgcg gagccaatcg aggtcgggct tgtcgggcag
     2641 agacatcgga caccttcctt ccttagaaag ggagcgatcc ctttcagccg cccgttgtcc
     2701 gcagaaaggg cgaggaacag aaggttatga acgaacgacg aagggcgggt tgaacccttt
     2761 acgcggaccg tcggccgccc tcgcggcgct ggctgaat
//
LOCUS       DBNGSH010000464         3462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3608770, whole genome shotgun sequence.
ACCESSION   DBNGSH010000464 DBNGSH010000000
VERSION     DBNGSH010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3608770"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="AC16CY_10840"
     CDS             complement(<1..199)
                     /locus_tag="AC16CY_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR3354330.1"
                     /translation="MSKSFATQIRQTRPFASPEQEAMLAIRRTAASVLAPWEKFLKAK
                     FGLSTSLFNVLRILRGSHPTAL"
     gene            complement(430..2865)
                     /locus_tag="AC16CY_10845"
     CDS             complement(430..2865)
                     /locus_tag="AC16CY_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354331.1"
                     /translation="MALFAPELADALRSLRQHPGFLLTAAGTLAIGICANVTVFSMVN
                     GVLLRPMPFGERTDRVMTLHSTHRLQAEDLDDSGVSSADLLDVRGETRSFEEVGGYVV
                     RNFTIASAIDTERLTGLSVTPNLFSMLGIEPALGRTFTPADAAAVGLESSVILTHGVW
                     QNHYGGDPNIIGRVVIINDRPQTVVGVMPPGFRFPQRAQLYAPLVLEGDTARTIRNVS
                     GIALLKPGVTRQQAQADVDAVAARLESAFPTTNRGYGIRVLTFRDSQVAPQTRIAMGA
                     LMTAVVFVLLIACANLANLLFIRGAARQRDMAIRAAMGATRARLIGHVFAESAIIAGA
                     GTAVGLSGALWCIGFLPSVWPEEFPYWLRLDPDVRLVAFTIGLMIFTTLAVGLLPAIR
                     MSRPSVTDALSHGGRTSSLGRSSHRVQRALAVGQVALCLALLVGAHLMIRSFLSLQRA
                     NLGFDERPLLSLRVFATGDAFDPIPARAALFARALDSLRALPGVAAVAATSAVPGDDG
                     GAMIRVTKDGAEGPFVGAQAITVTPGLFDTLAVRLEQGRTFTENEFANPDSDAVVINE
                     GLARQMWPGQSALERRVGIATADPRTDGVSWRRVVGVAPDLVYEELGEQTEQARLNVY
                     FPYARSPLRTMALLIRGDGNPATLITPARQALRQVHRGFAAFDVATMSERRRLTTGAQ
                     GALGTMMGGFAAAALLLACLGIYGLLADTARQRTQEIGVRMALGATPRGIVALFVGQA
                     AIIGAAGLVMGAVLAVLVAQALSGVLFGIDPLAVTPMVLSAATLIVVVILAAYVPARR
                     ASKVAPLVALRTL"
     gene            complement(2994..>3462)
                     /locus_tag="AC16CY_10850"
     CDS             complement(2994..>3462)
                     /locus_tag="AC16CY_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354332.1"
                     /translation="EPPKNPSPAAAALLARGEQVFGREGCVNCHVPPNYTSGRLTVTQ
                     GWEPPANHPNGEDIANVSVGTDPGLALKTRKGTGFYKIPSLRGVWYRPRLLHDASVTS
                     LEEMFDPARTSPEYEPKGWNPPGVTKRAVPGHAFGLSLNAEDRSALLAFLRSL"
BASE COUNT          671 a         1126 c         1111 g          554 t
ORIGIN      
        1 tcaaggccgt gggatgactg ccccggagaa tgcgcagcac attgaatagg ctggtggaca
       61 agccgaactt ggccttgagg aacttctccc acggcgcaag tacgctcgcc gccgtccggc
      121 gaatggccag catcgcttcc tgctccgggg aggcgaaagg gcgggtctgc cggatctggg
      181 tggcaaagct cttggacacc ggcagaacat tatctgttcc aacgactatt gtcaagcgga
      241 gttaaaactc ccgaacggta cctacggccg atggcgtaca cacttgccgt ccccgtggcg
      301 tctgaacgtc aggatcacgc ggcgatcccg acaaattcca caatctccgt ggaacaccct
      361 ttgtcataac cccgtaccgg taccaagaaa cactccgcat tcacactcaa gaccatttcg
      421 ggtagcggcc tataaggttc tcagtgcaac gagcggcgcg accttcgatg ctcggcgggc
      481 cgggacgtac gccgccagga tcactaccac gatcagggtc gcagcgctga gcaccatggg
      541 cgtcaccgcg agcggatcga tcccgaacag cacgccgctg agcgcctgtg cgacgagcac
      601 tgcgagtacg gcccccatga ccagaccggc cgcgccgatg attgcggctt gcccgacgaa
      661 cagggcgacg atgccccgcg gcgtggcgcc gagtgccata cgcacaccga tctcctgtgt
      721 gcgttgtcgc gccgtgtcgg caagcaatcc gtagatgccg aggcaggcca gcagcagtgc
      781 ggcggccgcg aagccgccca tcatcgtgcc gagggcgcct tgcgccccgg tcgtcagccg
      841 acggcgttcg gacatcgtcg cgacgtcgaa cgccgcgaat cccctgtgga cctggcgaag
      901 cgcctgccgc gccggtgtta tgagcgtcgc ggggttgccg tccccgcgaa tcagcaacgc
      961 catcgttcgc agtggactgc gcgcgtacgg gaagtagaca ttcagccgtg cttgttccgt
     1021 ctgctcgccg agttcttcgt agacgagatc tggggcgacg ccaacgacgc gccgccatga
     1081 cacgccgtcc gtccgcggat ctgcggtggc tatgccgacg cggcgctcga gagccgactg
     1141 gcctggccac atctgtctcg cgagcccttc attgatcacg acagcatccg agtcaggatt
     1201 cgcgaattcg ttctccgtga acgtacgccc ctgttcaagg cgaacggcaa gcgtgtcgaa
     1261 cagcccggga gtcaccgtga tcgcctgagc gccaacgaac ggcccttcgg cgccatcctt
     1321 cgtcacgcgg atcattgctc cgccatcgtc tccaggcacg gcactggtcg ccgccaccgc
     1381 cgcgactcct ggcagtgccc tcaaggagtc cagcgcgcgg gcgaacaacg ccgcgcgagc
     1441 gggaatcgga tcgaaggcgt cacccgtcgc gaatacgcgc agtgacagca gtggacgttc
     1501 gtcgaagccc agattggcgc gctggagcga caggaaactc cgaatcatca agtgcgcgcc
     1561 gacgaggagc gccagacaca gtgccacctg ccccacggcc agcgcccgct ggacgcgatg
     1621 cgaggagcgt cccagcgagg aggtccggcc gccgtgcgac agcgcgtcgg tgacactcgg
     1681 acgcgacatc cggatcgccg ggagcaggcc gaccgctaac gtcgtgaata tcatgaggcc
     1741 gatggtgaac gcgaccagtc ggacgtcagg atccagtcgc agccaatacg ggaactcctc
     1801 gggccaaaca gaaggaagga agccaataca ccatagcgcg ccggagagac cgaccgccgt
     1861 gccggcgcct gcaatgatcg cgctttcagc gaacacgtga ccgatcaggc gggcgcgggt
     1921 cgcacccatc gcggcgcgga tggccatatc gcgttgccgc gcggcgccgc ggatgaacag
     1981 caggttcgcc agattcgcgc acgcgatcag cagcacgaat acgacagcgg tcatcagggc
     2041 gcccatcgcg atgcgcgtct gtggggccac ttgcgagtcg cggaacgtga gcacgcgaat
     2101 gccgtagccg cggttcgtcg tcgggaacgc cgattccagc cgcgccgcga cggcgtccac
     2161 gtccgcctgc gcctgctgcc gggtaacccc gggcttcagc agagcgatgc ccgatacgtt
     2221 gcgaatcgtt cgcgcggtat cgccctcgag aaccagtggc gcatagagct gcgcgcgctg
     2281 gggaaagcgg aatccgggcg gcatgacgcc cactaccgtc tgagggcggt cgttgatgat
     2341 cacgacacgg ccgatgatgt tcgggtcgcc cccgtagtgg ttttgccaga caccatgcgt
     2401 aaggatgacg gagctttcaa ggcccacggc ggccgcatca gcgggcgtga aggtgcgtcc
     2461 cagcgcgggc tcgattccca acattgaaaa gaggttgggt gtcaccgaga gccccgtcaa
     2521 gcgctcggta tcgattgctg acgcgatggt gaaatttcgg acgacatagc caccgacctc
     2581 ctcgaacgat cgggtctcac cacggacgtc caggagatcc gccgaggaga cgccagagtc
     2641 gtccaagtcc tctgcctgaa ggcgatgcgt cgaatggagc gtcatcacgc ggtcagtacg
     2701 ctcgccgaac ggcatcgggc ggagtaggac gccattgacc atgctgaaca cggtcacgtt
     2761 cgcgcaaatg ccgatggcga gagttccggc agcggtcaag aggaatccag gatgctgacg
     2821 gaggctacga agcgcgtcgg caagttcggg cgcgaacagg gccatggccg cctcacgggc
     2881 aaggcccggg ccaagagctg cgcgtcggtc gacgggccga accatcgtcg gaaggctaca
     2941 agttgtgtcg ccagtgggat gacgtgtccg ataccggacg cgcgcgcgct cacttaaagg
     3001 cttcgcaaga acgccagcaa tgcgcttcga tcttcggcat tcaacgacag accaaaggca
     3061 tgcccgggca cggcgcgctt cgtcacgccg ggcggattcc agcccttcgg ttcgtactcg
     3121 ggtgaggtac gcgccggatc gaacatctct tcgagactcg tcaccgaggc atcgtgaagc
     3181 aagcgcggcc ggtaccagac gccgcgcaac gagggaatct tgtagaagcc cgttcccttg
     3241 cgtgtcttga gtgcgaggcc gggatcggtt cccaccgaga cgttcgcaat gtcctcacca
     3301 ttgggatgat ttgccggtgg ctcccagccc tgggtgactg tgagcctgcc gctcgtgtaa
     3361 ttcggcggca cgtgacaatt cacgcaacct tccctgccaa aaacctgttc acctcgcgcc
     3421 agcagtgccg ccgcagccgg gctcggattc ttcggcggct cg
//
LOCUS       DBNGSH010000465         7357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2929671, whole genome shotgun sequence.
ACCESSION   DBNGSH010000465 DBNGSH010000000
VERSION     DBNGSH010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2929671"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="AC16CY_10855"
     CDS             complement(<1..214)
                     /locus_tag="AC16CY_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354333.1"
                     /translation="MTRHLAVVPMLLLVLGSGGQSQSAPTLRFVSPTDDTYLSGPVLL
                     KVVIEGEAGPSLVEDVTFFANGREVCV"
     gene            complement(211..3195)
                     /locus_tag="AC16CY_10860"
     CDS             complement(211..3195)
                     /locus_tag="AC16CY_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354334.1"
                     /translation="MPVPPASLALLDVGVEQGRSLAAQRAIRVLHSSARDADRHARAV
                     EFEQLLTALDAFEVQMLRTGTRGLSLAMATNSSERDVLRDVLEVMGIRLRERRGAYSA
                     EPDDDDEAVSLRKRLDSAGIDTSAVVKRLNAGETVTPSSTVVDLPSPLPLETWTSVVF
                     ERSLPPRSVFSAIARDRQASLLFYGLQTMTPRTRAFVAGNRELLQRLYRDLAGSVAAF
                     GRSFRVDDRGRVVVPGGLEAEDVWKELVGEPLERADRFARALFDRDAGRLAYFFDAIA
                     HLDAPRQRFALGLWVSNPEIRLDRFRALYRTFVDVDSPWVAPERPFMRPLYDPATLLA
                     LAAVGQEGEPASPSYRRLWERAFEAVDLPDPGDRLLGDVSEDGVVDAAWLVEHAMRDA
                     FSERRALFERLMSGHRVFGGVREQELEDVLVALRGFGRFPALMLTLERIGIRRPALFA
                     LVARQARHTEGVSDQALAVRLLTQFQGALALLDRLARTGAVAAPRLEELVSSLAALPI
                     DDRGYRGALAAWVNNSLLPSLPARDPDGARPLESSLLRALAETGEDGSPFAWEGESYV
                     TDIASASLRELVAIRSKQGGNSLDDVLALFGHAAALGQNGLTIESIKPHAASIASTSA
                     KLSAARPWPDVPDEVPNIKRILDRAARDLGRIQKPQDVSRAPRIAEPIVALAEDLLGE
                     TLVALAYAPFLGDPEELLGPQSDVSHRHRFGLAARPNAPSTERQAWRRPVVDGDGVGL
                     GYTGSLLGLDLALANRRLRRLSTNTVPAAPRISPNDGAALVESLALLNSRSLATADLR
                     SIGEAVTRGRERVRTSRTNPAVLDELANAVQMSDARRQLLAWTQEKEPDRIEALFAPS
                     ELLRLGLADRQLERIDAWGGSYEAQSGCYCSRFPPAGAWDRLAGRPGTGQLGPAVASE
                     LILRVAEHLAALGVPAGVATAVLAIAAQDYIDGAPPIFEDDWLGLVGHAQLVSRESVE
                     DYVAAAVAAGPARAAAPRESSK"
     gene            complement(3463..4491)
                     /locus_tag="AC16CY_10865"
     CDS             complement(3463..4491)
                     /locus_tag="AC16CY_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3354335.1"
                     /translation="MSVLGWDIGGVNTKVAHVARGGVVAVRSQPYEMQREPRAFVPLL
                     RGLAAGIGSGEQAPHALTMTAELSQMFRTKREGVRFVLDGVEAAFPSALIRVYTVTGQ
                     FLTPARARERPLEVAAANWSATARAVATQHPDALLIDIGTTTTDIIPIAGGRVAATGR
                     TDPERLASSELVYTGALRTPAEAIASHVPLGDEPAGVSAEGFALAGDVHVWRGDLDPA
                     DYTCPTPDGRPSSREFAGERLARLVCADREMLDEAAISRIAETLARAQIRRIQAAVQR
                     VLDRHPSIRVAVVTGLGAFLGASAVQGTGLGVVPLSTRLGDAGARCAPAVSVALLLEG
                     ELAGPAAG"
     gene            complement(4652..>7357)
                     /locus_tag="AC16CY_10870"
     CDS             complement(4652..>7357)
                     /locus_tag="AC16CY_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3354336.1"
                     /translation="TRRQTVIDDELLAAIPSARSYGGLIMLMPNTQNFAGSAADTQVV
                     PQMVVFGGAGGRSNEGRLSIDGLSVGSAFNGAGVSAYVADVGNAQEIALTSSGGLGEA
                     EVGGPSLNVVPREGGNTVRGSFYGSGVTEGMVGSNFTQELEDRGMSTPGAYQQIWDFN
                     LGVGGPIASDRLWYFGTFRNEGSHRTIPSMFANANAGDPTKRTYVQDASRRAVAAVSF
                     QNIALRLTAQVTPRNKVTFFWDEQMPCEGAALAGLGDDADACRHSDSDEIIAGPGIPP
                     TPTASATAAPETAAYRDYGQRVRQAKWTSPMTNRLLIEGGVGQYRSRYGGSPMPGANT
                     QDLIRVVEQCAGAAGCPLNGGIPNLAYRSPNWSSNVNQNVNWNAAGSYVFGGQSLKVG
                     YQGALLYDNRKAFSNSHFLQYRVNNAVPDQLTMTINQYETRQRVRFDAFYVQDQWTMG
                     RITLQGALRYDHAWSYFPEQTVGPVRFFPTPVTYPETPGVEGYHDLTPRGGFAMDLFG
                     NGKTSLKVNFGRYLEAAQNMGYFTALNPTARLSTTTTRSWTDSDDDFVADCDLNNPGA
                     QDLRPVGGDLCGANTNTNFGKELFESTLDPALVSGWGVRTGDWQWGASIQHEVFPRVS
                     VEGGYQRRWLVNFTATDNFSRAPEDHTEFGVNVPSDVRLPDGGGYVLGGLYNVTPTAA
                     LRLNNNVLTQASGYGDWSQVTDSVNFNVTARPRNGLTLQGGFNLGTTNVDYCQVRAEL
                     PEMNVILTTISPTSRWCAYSTEFLRFTALGSYVIPKIDVQIGGAMRSDQGGDLAANWA
                     APNSATVGLNRPFAGVGGQTVTVNLVEPGTLYGDRVNQIDLRIAKILRFGRTRTNVGV
                     DIYNIGNVNPVLTYNQAYNPATTAWLTPNSVLQPRFFKISAQVDF"
BASE COUNT         1223 a         2512 c         2443 g         1179 t
ORIGIN      
        1 ccacgcacac ctctcgaccg ttggcgaaga aggtcacgtc ttcgaccagc gagggtccgg
       61 cttcgccctc gatgacgacc ttcaagagca ccggtccgct gaggtacgtg tcgtcggtcg
      121 gcgaaacaaa gcgtagtgtc ggcgcggact gcgactgccc tcctgacccg agaacgagca
      181 acagcatcgg cacgacggcg agatgacgcg tcatttcgac gactcccgcg gcgcggcagc
      241 ccgtgctggt cctgcggcca ctgcggctgc gacgtagtct tcgaccgatt ccctcgagac
      301 gagctgtgcg tgaccgacga gaccgagcca gtcgtcttcg aagatcggcg gcgcgccgtc
      361 gatatagtcc tgcgcggcga tcgccagcac cgcggtggcg acaccagcgg gaacgccgag
      421 cgcggccaga tgctcggcca ctcgcaggat caactcgctg gccacggctg ggccgagctg
      481 ccctgttccc gggcgtccgg cgagacggtc ccaggcgccc gccgggggaa agcgcgagca
      541 gtagcagccc gactgcgcct cgtatgaccc tccccacgca tcgatgcgct ccagttggcg
      601 atcggcgaga cccagtcgca gcaactccga cggcgcaaag agcgcttcga tccgatcggg
      661 ctccttctcc tgcgtccatg cgagcagctg ccggcgcgca tcgctcatct ggacggcgtt
      721 ggcgagctcg tcgagcacgg caggattggt tctcgaggtc cgcacccgct cgcggccgcg
      781 tgtcaccgcc tcgccgatcg agcggaggtc ggcggtggcc aggctccggg aattcaagag
      841 ggcgagagac tcgacgagcg ccgcgccgtc gttcggactg atccgcgggg cagcgggaac
      901 cgtgttcgtg gaaaggcgcc gcagacgcct gttggccagt gcgaggtcca gaccgagcaa
      961 cgaaccggtg taccccaggc cgacgccgtc gccgtcgacg accggccggc gccaggcctg
     1021 acgctcggtg gaaggcgcat tcggccgcgc agcgagtccg aacctgtggc gatgggacac
     1081 gtcggactgc gggccgagca gctcctctgg atctccgagg aacggcgcgt aagcgagcgc
     1141 cacgagcgtc tcgccgagaa gatcctccgc gagcgcgacg attggctccg cgatgcgcgg
     1201 cgccctcgac acatcctgcg gcttctgaat cctgcccagg tcgcgtgccg ctcgatcgag
     1261 gattctcttg atgttcggaa cctcgtccgg gacgtccggc cacggacgcg cggcagacag
     1321 tttcgcgctc gtgctcgcga tcgacgccgc gtgcggcttg atcgactcga tcgtgaggcc
     1381 gttctgcccg agcgccgccg cgtggccgaa cagcgcaagc acgtcgtcca ggctgttgcc
     1441 gccctgcttc gaccggatgg ccacgagctc gcgaagcgag gcggacgcga tgtcggtcac
     1501 gtaggactcg ccttcccacg caaacggcga accgtcctct cccgtctctg ccagcgcgcg
     1561 aagaaggctc gattcgagcg gacgggcgcc atcgggatcg cgcgcgggga gcgacggcag
     1621 gaggctgttg ttgacccagg ccgcaagggc accgcggtag cctctgtcgt cgatcggcag
     1681 agccgccagc gacgagacga gctcttcgag ccgcggcgcc gcgaccgccc ccgtccgcgc
     1741 caggcggtcc agcagcgcga gcgcgccctg aaactgcgtc agcagccgca cggccagcgc
     1801 ctggtccgac acgccttcgg tgtgacgggc ctggcgcgcg acgagcgcaa acagcgcggg
     1861 ccggcggatg ccgattcgct cgagcgtcaa catcaacgct gggaatcgcc cgaagccgcg
     1921 gagcgccacg aggacgtcct cgagctcctg ctcgcggacg ccgccgaaca cgcggtgtcc
     1981 agacatcagc cgctcgaaga gcgcgcggcg ctccgagaac gcgtcgcgca tcgcgtgctc
     2041 gacgagccag gcggcgtcaa cgacgccgtc ttcggacacg tcgcccagga ggcgatctcc
     2101 gggatcgggc agatccaccg cctcgaaggc gcgctcccac agtcgccggt acgaggggga
     2161 cgccggttcc ccctcctgtc cgaccgccgc cagcgccagg agcgtcgcgg gatcgtagag
     2221 cggcctcatg aacgggcgct ccggagccac ccacggcgag tccacatcga cgaacgtgcg
     2281 atacagcgcg cgaaatcgat cgagccggat ctcgggattg ctgacccaca gtccgagcgc
     2341 gaaccgctgg cgaggagcgt cgagatgcgc aatcgcgtcg aagaaatacg ccagccggcc
     2401 cgcgtcacga tcgaacagcg cgcgtgcgaa ccgatcggca cgctccagag gctcgcccac
     2461 gagctccttc cacacgtcct ccgcctcgag acctcctggc acgacgacgc gtccccggtc
     2521 gtcgacgcgg aacgagcggc cgaaggcggc caccgatccg gcgagatcgc gatagagacg
     2581 ctggagcagc tcgcggttgc ccgccacgaa ggctcgcgtt cgaggcgtca tcgtctgcag
     2641 cccgtagaag agcagagacg cctgtcgatc gcgcgcgatg gcgctgaaca cggaacgcgg
     2701 cggaagagat cgctcgaaca ccacgctcgt ccacgtctcg agcggcagcg gcgacggcag
     2761 gtcgacgacg gttgacgacg gcgtcaccgt ctcccccgcg ttgagccgct tcacgacggc
     2821 cgacgtgtcg atgccggctg aatcgagccg ctttcgaagc gacacggctt cgtcgtcgtc
     2881 gtccggttct gctgaatacg ctccgcgccg ctctcgaagg cggatcccca tgacctcgag
     2941 cacgtccctg aggacgtcgc gttcggaaga attcgtcgcc atcgcgaggc tgaggccgcg
     3001 tgtcccggtc cgcagcatct ggacctcgaa ggcgtccagg gccgtgagca actgctcgaa
     3061 ctcgacggcg cgcgcatgcc ggtcagcgtc gcgggcgctc gagtgcagaa cgcgaatcgc
     3121 gcgttgggcg gcaagcgatc gcccctgctc cactccgacg tcgagcaagg cgagcgacgc
     3181 gggcgggacg ggcatgtcga accaggcaag gggctccgcc ctttgatatc ccgcggagcc
     3241 ctttgcctgg tcgacagtgt ctctttggcg tgctacgcgc gccgggaccg ctatgcggaa
     3301 acgcaccgag tgcaggattc gcgtttcggg ccgcatagca gtccataccg gctgcgcccc
     3361 tgccgccgtc gtcacacaca gcaagaacag cgcgagaacg agcgtcttca tcgagcccgc
     3421 agattctacc cgttcggggt caccgacagt ggatctcgcg cgtcaacccg cggccgggcc
     3481 ggccagctct ccttcgagta ataacgcgac ggagaccgcc ggcgcacacc gcgcgccggc
     3541 gtcgccgagg cgagtcgaaa gcggaacgac ccccaacccg gtcccctgca ccgccgacgc
     3601 gcccagaaac gcgccgaggc cggtcaccac cgcgacgcga atggacggat gccggtcgag
     3661 cactcgctgg accgcggcct ggatgcggcg gatctgtgcc cgggccagcg tttcggcaat
     3721 cctcgatatc gcggcttcat ccagcatctc gcggtcggcg cacaccagcc gcgccagccg
     3781 ctcccccgcg aattcgcggc tggaggggcg gccgtcaggc gtcgggcagg tgtagtcggc
     3841 cggatccagg tcgccgcgcc agacgtggac gtcgcccgcg agcgcgaacc cttccgccga
     3901 cacgccggcc ggttcgtcgc ccagcggaac gtggctcgca atcgcctcgg caggtgtccg
     3961 cagcgcgccg gtgtagacca actcggacga ggccaggcgc tcggggtcgg tgcgccccgt
     4021 cgcggcgacc cggccacccg cgatcgggat gatgtcggtg gtcgtcgtgc cgatgtcgat
     4081 cagcagcgcg tccggatgct gcgtggcaac cgcgcgcgcg gtcgccgacc agttcgcggc
     4141 cgcgacctcg agcggacgct cgcgagcgcg ggcgggcgtc aggaactgcc ccgtcaccgt
     4201 gtagacgcgg atgagggccg acgggaacgc cgcctcgact ccgtcgagca cgaaccgtac
     4261 gccctcgcgc ttggtccgga acatctgcga cagttccgcg gtcatcgtca acgcgtgcgg
     4321 agcctgctcg cccgatccga ttccggcggc gagccctcgc agcagcggga cgaaggcccg
     4381 gggctcgcgc tgcatctcgt acggctgcga gcggacggcc acgacgccgc cacgagccac
     4441 gtgggcgacc ttggtgttca cccccccgat gtcccagccg aggacggaca cacggtcaat
     4501 ctaccgcatg aatcggattc cgaagcgtcg cgaagccacg aagatcacga cggtttcgcc
     4561 acgaagcgca cgaagatcac gaagggattt cgtgtttcgt gttgtttctt cgtgtcttcg
     4621 tggccaggtc ttcgtacttc gtggctctgc ctcagaagtc gacctgcgcg ctgatcttga
     4681 agaagcgagg ctgcaggacg gaattcggcg tcagccacgc ggtggtggca ggattgtagg
     4741 cttggttgta ggtcagtact ggattgacgt ttccgatgtt gtagatgtcg acgccgacgt
     4801 tggtgcgcgt gcggccgaac cgcaggatct tcgcaattcg caagtcgatc tgattcacac
     4861 ggtcgccgta aagcgtgccc ggctcgacga ggtttaccgt taccgtctgg cctcctactc
     4921 ccgcaaaggg gcgattgagg cccaccgtgg ctgaattggg cgcagcccag ttggcggcca
     4981 gatccccacc ctgatcgctg cgcatcgcgc cgccgatctg cacatcgatc ttgggaatca
     5041 cgtaggagcc cagcgccgtg aagcgcaaga actccgtcga gtacgcacac cagcggctgg
     5101 tcgggctgat cgtcgtgaga atgacgttca tctccgggag ctcggcccgg acctggcaat
     5161 agtcgacgtt cgtcgtgccg aggttgaagc ccccttgcag cgtgagtccg tttcgaggcc
     5221 gtgccgtgac gttgaagttg accgagtctg tcacctgcga ccagtctcca tacccgctgg
     5281 cctgcgtgag gacgttgttg ttgagccgca gagccgcggt tggcgtgacg ttgtacaacc
     5341 cgccgaggac gtagcctcca ccgtcaggga gccgtacgtc gctcgggacg ttgacgccga
     5401 actcggtgtg atcttccggc gcgcggctga aattatcggt cgccgtgaag ttcaccagcc
     5461 agcgccgctg ataaccaccc tcgaccgaca cgcgaggaaa cacctcgtgc tggatggagg
     5521 cgccccactg ccagtcgccg gtccggaccc cccatcccga gacgagcgcc gggtcgagcg
     5581 tcgactcgaa cagctccttg ccgaagttcg tgttcgtgtt ggcgccacag aggtcccccc
     5641 cgacggggcg gagatcctgc gcaccgggat tgttgaggtc gcagtcggcg acgaagtcat
     5701 cgtctgaatc ggtccatgat ctcgtggtcg tcgtggagag acgggcggtg ggattgagcg
     5761 ccgtgaagta ccccatgttc tgggcggcct ccagataccg gccgaagttg accttcagcg
     5821 atgtcttccc gttgccaaac aggtccatcg cgaagccgcc gcgcggggtc aggtcgtgat
     5881 acccctcgac gcctggcgtt tcgggatagg tgacgggcgt tggaaagaac cggaccggtc
     5941 caaccgtctg ctcggggaag tagctccagg cgtggtcgta gcgcaacgcg ccctgaagag
     6001 tgatgcgtcc catcgtccac tggtcctgca cgtagaacgc atcgaaacgg acgcgctgcc
     6061 gtgtctcgta ctggttgatg gtcatcgtca gctgatcggg gacggcgttg ttcacgcggt
     6121 actgcaggaa atggctgttc gagaaggcct tccgattgtc gtacagcaac gcgccctggt
     6181 agccgacctt caggctctgc ccgccgaaga cgtacgatcc ggccgcgttc cagttgacgt
     6241 tctgattcac gttcgagctc cagttcggcg atcgatacgc caggttcggg atgccgccgt
     6301 tcagcgggca tcccgcggct cccgcacact gctcgacgac gcgaatgagg tcttgagtat
     6361 tggcacccgg catcgggctg ccgccgtaac ggctccggta ctgcccgacg ccgccttcga
     6421 tgagcaggcg gttcgtcatg ggagacgtcc acttggcctg ccggacccgc tgtccgtagt
     6481 cccgataggc cgccgtttcg ggcgccgccg tggccgacgc ggtcggcgtc ggcgggatgc
     6541 ccgggcccgc gatgatctcg tctgaatcgg agtgcctgca cgcgtcggca tcgtcgccca
     6601 gccccgccag tgcggctccc tcgcacggca tctgctcgtc ccagaagaag gtgaccttgt
     6661 tgcgcggtgt gacctgcgcc gtcaaacgca gggcgatatt ctgaaacgat acggcagcga
     6721 cggcccgtcg tgaggcgtcc tgcacatagg tccgcttggt cggatcaccg gcgttggcat
     6781 tcgcgaacat gctgggaatg gtccggtggc tgccttcgtt tcggaacgtc ccgaagtacc
     6841 acagcctgtc gctggcgatc gggcccccga cgccgaggtt gaaatcccag atctgctgat
     6901 acgcgcctgg cgtcgacatc ccgcggtcct ccagctcctg ggtgaagttg ctgccgacca
     6961 tcccctcggt cacgcccgat ccgtagaagc tgccgcgaac ggtgttgccc ccctctctcg
     7021 gtacgacgtt caacgacgga ccgccgacct ccgcctcgcc gagcccgccc gaggaggtca
     7081 gggcgatttc ctgggcattg ccgacgtcgg ccacatacgc cgacacgccg gcgccgttga
     7141 aggccgaacc gacgctgagg ccatcgatgc tgaggcgccc ctcgttgctc ctgccgcctg
     7201 caccgccaaa gacgaccatc tgtggcacca cctgcgtgtc ggcggccgag cctgcaaaat
     7261 tctgcgtgtt cggcatcagc atgatgagtc caccgtagga cctggccgac gggattgcgg
     7321 cgagcaattc atcatcgatg accgtttgcc gcctggt
//
LOCUS       DBNGSH010000466         5033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_212872, whole genome shotgun sequence.
ACCESSION   DBNGSH010000466 DBNGSH010000000
VERSION     DBNGSH010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_212872"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..716)
                     /locus_tag="AC16CY_10875"
     CDS             complement(96..716)
                     /locus_tag="AC16CY_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR3354337.1"
                     /translation="MARFRLIPREERFFEDFVGMAEEIRGGARLLKQMLASDPPDAAK
                     ADAIKEVEHVCDGRTRAIIDRLNRTFVTPLDREDIHALAISLDDVMDAIDAAAAVVRL
                     YKITQVRAGARRLTEIICDSMDRLCEALAVLEKQHGVRELAARVDQLEREADRVHQDA
                     IVALFDEERDPIVVIKWKEIFDFLEAATDRCEDVANLLEGVVVKHG"
     gene            821..4522
                     /locus_tag="AC16CY_10880"
     CDS             821..4522
                     /locus_tag="AC16CY_10880"
                     /inference="COORDINATES: protein motif:HMM:NF015194.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HIR3354338.1"
                     /translation="MIARLLKPFVQIRSGEAATVLLMFAYSFLAMAGYNMIKPVTRGL
                     FIEKYSAEDLPWVQLGAGIVIGFIMQGYSRVIARVPRRWMIPITLSAITVVTAAFYGM
                     FDAWPANRGIAVGFYLFGLIVGVLLISQFWTLANDVYDPRQAKRVFGFIGAGASLGGY
                     AGGKATQEILERIGDNVNPVLLVSAVILAVCTFTAWAIIAREPNAGQSDASKTGEEGG
                     VSGSEAIRLLRSSRHLQTIAMVIGFAAIGAAIIEQQLNMAAAQQKGAANVGAVTAFLS
                     QIGAYTSLIGFVIQILLTSRIHRYLGIGFALLIIPVVDASTGLLMLLTGALWTSGLAR
                     VMDTSLRYTVDKTTREILFLPLPVDIKYQAKPFIDVTVDRLAKAVGALVLLVLVQPWG
                     LRLGWPQLSYAGVTMMAIWATFALTARREYLRSFRRSIEQQSVKPSDIRLESADLSSI
                     ETLVTELAHPDPRRVIYAIDMLDALDKRQLVTPLLLHHNSAEVRARALHVAKASGADG
                     AERWLPMVEQALTDEDGDVRLAAARALATIHGGSAADVMRPFLASPDPNLVITAAAAL
                     ASSDNEADVAAAEGTFKRYASDTRAAAASLRQQVARALGLVQNARLRSLLVPLMHDPD
                     YVVARAAIESARTIAGDDFLFVPTLVSLMRNRRLKSAARQVLIEHGEAVVAPLAYFMS
                     DQDEDIWVRRHVPEALALIPTPSSVAALIAALDADDGFLRFKAVAALQHLRRDHPGLA
                     VDRSVIERRVLREAARAFDTLTLYTNLFVAGDLNRDSLLARALQEKRGHALNRTFRLL
                     SLIHPAGDIAAVQSSLAMADARTRSGAIELLDNLLAGEVRKRVMLLVDEMPSDERVRK
                     GNTLYKTRPRDIEDTLAQLIHDENQVISAAAISVVEERRLWSLSADLEHVLAHRDPRD
                     WYCFEAASWALAAQRMPIDRRKQLWQEPLPAVELADRLRKLAIFRFTSVDELFRMAAV
                     GRQVRHETNRLIYDRGAAPDSLQLLLDGRVAVSGLSQEELMPPAPLAFEAVLEGRPVA
                     SEVRALEPSICLSLTNDEFLSVLAENIELTEGIFRWIIESRKLRSRGAVSRGTLTPEL
                     KRKMSAGLQPVDRALLLQVSPVLSCATGAQVLRLAGIARPVSFKAGSTPFAGYAEPGM
                     LALLTGAVEARSPDGRIERAEQGDMIGVLETLGGLPLDGTLVAASDGTALRVNRSDLF
                     DLFADDTTLVQGIASGLLSAGERVEARPR"
     gene            complement(4527..>5033)
                     /locus_tag="AC16CY_10885"
     CDS             complement(4527..>5033)
                     /locus_tag="AC16CY_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354339.1"
                     /translation="ADERSLLRHTLATLAYRAEKVLRDPPTGFADHRLAPDSRSPLEI
                     VGHLGDLLEWAEQLAQGHWVWKAASAGTWDVDVERFFATLARLDAHLASDTPLGHPAA
                     IIFQGPIADALTHVGQLAMLRRLAGSPVRPESYGRAQIHAGRVGRQQSSERREFDGDA
                     SALPARTR"
BASE COUNT          810 a         1657 c         1696 g          870 t
ORIGIN      
        1 ttgatgtagt cgaagatcag cgcaaccgcg atgagggcga gaacggcgag cagggtgtga
       61 tccatggagt ggagacgcgt cgctctggcg aagccctagc cgtgcttgac gacgacgcct
      121 tcgagcaggt tggcgacgtc ctcgcaccgg tcggtggcgg cctcgaggaa gtcgaagatc
      181 tccttccact tgatcacgac gatcgggtca cgctcctcgt cgaagagcgc gacgatggcg
      241 tcctgatgca cgcggtcggc ctctcgctcg agctgatcga cccgcgccgc cagctccctg
      301 acgccgtgct gcttctcgag gacggccagc gcctcgcaga ggcggtccat cgagtcgcag
      361 atgatttcag tgagccgccg cgctccggcc ctgacctgcg tgatcttgta caggcgcacc
      421 accgccgctg ccgcgtcgat cgcatccatc acatcgtcca gcgagatggc cagcgcgtgg
      481 atatcttcgc ggtcgagcgg cgtgacgaag gtccgattga ggcggtcgat gatggccctg
      541 gtgcgcccgt cgcagacgtg ctcgacctcc ttgatggcgt cggccttggc ggcatccggc
      601 ggatcgctcg ccagcatctg cttgagcagc cgcgcgccgc cccggatctc ctcggccatg
      661 ccgacgaaat cctcgaagaa gcgttcctcg cggggaatca ggcgaaatcg tgccatgtca
      721 cctcggtacc ggttcgggag cccactcgca cctggcgggt gggagagagc cctcggatcg
      781 tagcagaatt acggttcagc ttcgtcgtaa gatccgctcc gtgatcgcgc gcctcctgaa
      841 gcctttcgtg cagatccgca gcggcgaagc cgcgacggtg ctgctgatgt tcgcctactc
      901 gttcctggcg atggccggct acaacatgat caagccggtc acgcgcggat tgttcataga
      961 gaagtacagc gcggaggacc tcccctgggt ccagttgggc gcgggcatcg tcatcggatt
     1021 catcatgcag ggctactcgc gggtgattgc ccgcgtgccg cgccgctgga tgattccgat
     1081 tacgctgtcg gcgatcacgg tggtgacggc ggcgttctac gggatgttcg atgcgtggcc
     1141 cgccaaccgc ggcatcgccg tcggcttcta cctgttcggt ctcatcgtcg gggtcctctt
     1201 gatcagccag ttctggacgc tggccaacga tgtctacgac ccgcgtcagg cgaaacgcgt
     1261 gtttggcttc atcggcgcgg gcgccagcct cggcgggtac gctggcggga aggcgacgca
     1321 agaaatcctc gagcgaatcg gtgacaacgt caaccccgtt ctgctggtca gcgcggtgat
     1381 actcgcggtg tgcacgttca cggcatgggc gatcatcgcc cgtgaaccga acgcaggcca
     1441 gagcgacgcg agcaagacgg gcgaggaagg cggtgtcagc gggtcggaag cgatccggct
     1501 tctgcgctcg tcgcgtcacc tccagacgat tgcgatggtg atcggttttg ccgcgatcgg
     1561 cgcggcaatc atcgaacagc agttgaacat ggcagccgct cagcagaagg gcgcagcgaa
     1621 cgtcggcgcc gttacggcgt ttctctcaca gatcggcgcc tacacgtcgc tgatcgggtt
     1681 cgtcatccag atcctgctga cgagccgcat ccaccggtat ctcgggattg gctttgcgct
     1741 gctgattatt cccgttgtgg atgcgagcac gggtctcctc atgctgctga ccggcgccct
     1801 ctggacctca ggcctcgcgc gcgtgatgga cacgtcgctc cggtacaccg tggacaagac
     1861 cacgcgcgaa atcctgttcc tgccgctgcc ggtggatatc aagtaccaag ccaagccgtt
     1921 catcgatgtc accgtcgatc gtctggccaa ggccgtcggc gcgctcgtcc tcctcgtcct
     1981 ggtgcagccg tgggggctga ggctgggctg gccacagttg agctacgccg gggtcacgat
     2041 gatggcgatc tgggcgacgt ttgcgctgac tgcgcgacgc gagtacctgc gatcgttccg
     2101 ccgcagtatc gaacagcagt ccgtgaaacc gtcggacatc cgtctcgagt cggcggatct
     2161 gtcgtcgatc gagacgctcg tcaccgaact ggctcacccg gacccgcgcc gcgtgattta
     2221 cgccatcgac atgctcgacg cgctggacaa gcggcagttg gtgacaccgc tgctgcttca
     2281 ccacaactcg gccgaggtcc gcgcacgcgc cctgcacgtg gcgaaggcct cgggagccga
     2341 cggcgccgag cgctggctgc ccatggtgga gcaggcgctc accgacgagg acggcgacgt
     2401 gaggctcgcc gcggcgcgcg cgctcgccac gatccacggc ggtagcgccg cggacgtcat
     2461 gcggccgttt ctcgcgagcc ccgatccgaa tctggtgatc acggcggcgg ccgcgctggc
     2521 ctcgagcgac aacgaagccg atgtcgccgc tgcggaaggc acgttcaagc ggtatgcctc
     2581 cgacacgcgc gcggcggcag ccagcctccg ccagcaggtg gcgcgcgcgc tggggctggt
     2641 ccagaacgcg cgcctgcgat cgctgctcgt gccgctgatg cacgaccctg actacgtcgt
     2701 tgcgcgcgcg gcgatcgaga gcgcacggac gattgcgggc gacgattttc tgttcgtgcc
     2761 cacgctcgtg tcgctgatgc gcaatcggcg gctgaagagc gccgcgcgcc aggtgctgat
     2821 cgagcacggc gaggcggtgg tggcgccgct cgcctacttc atgagcgacc aggacgagga
     2881 catctgggta cggcgccacg tgccggaggc attggcgctc atcccgacac cgtcgtcggt
     2941 ggccgctctg atcgcggcgc tggatgcaga cgatggattc ttgcgcttca aagccgttgc
     3001 ggcactgcaa cacctgcgga gggatcatcc cgggctggca gtcgaccgat cggtgatcga
     3061 acggcgcgtg ctgcgcgagg ccgcacgcgc gttcgacaca ctgacgctct acacgaacct
     3121 gttcgtcgcc ggcgacctca accgcgactc cctgctggca cgcgcgctcc aggaaaagcg
     3181 aggccacgcg ctgaaccgca cattccggtt gttgtcgctc atccaccccg caggcgacat
     3241 cgccgccgta cagtcatcgc tcgcaatggc cgacgcccgc acgcgttcgg gcgccatcga
     3301 gctgctcgac aatctgctgg ctggcgaggt gcgcaagcgg gtgatgctgc tggtcgacga
     3361 gatgccctca gacgagcgcg tgcgcaaggg aaatacgctg tacaagacac gcccacgcga
     3421 catcgaggat acgctggcgc agctcatcca tgacgagaac caggtgatct ccgcagcagc
     3481 catctccgtg gtggaggagc ggcggctgtg gtcgctctca gccgacctcg agcacgtgct
     3541 cgcgcaccgc gatccgcgcg actggtactg cttcgaggcg gcgtcgtggg cgctggcggc
     3601 ccagcgcatg ccgatcgatc gccgtaagca actctggcag gagccgctgc ccgccgtgga
     3661 gctggccgac cgcctgcgca agctcgcgat cttcaggttc acgtcggtcg acgagctgtt
     3721 ccgcatggca gccgtggggc ggcaggtacg acacgaaaca aatcggttga tctacgaccg
     3781 cggcgcggcg cccgattcgc tgcagctgct gctcgatgga cgcgtcgcgg tcagcggcct
     3841 ctcgcaggag gaactgatgc caccggcgcc gctggcgttt gaagccgtcc tcgaaggacg
     3901 ccctgtggcg tccgaggtcc gcgcgctcga gccgtcgatc tgcctgtcgt tgaccaacga
     3961 tgagttcctg tcggtgcttg ccgagaacat cgagctcacc gaaggcatct tccgctggat
     4021 catcgaatcg cggaagctgc gatcgcgtgg cgcagtgtcg cgcggcacgt tgacgcccga
     4081 actgaagcgc aagatgagcg cgggactgca gcccgtggat cgcgcgcttc tgttacaggt
     4141 gagtccggtg ctgtcgtgcg ccacgggtgc gcaggtgttg cgcctcgccg gcattgcgcg
     4201 cccggtgagc ttcaaggccg gcagcactcc gttcgccggc tacgccgaac caggcatgct
     4261 tgcgctcctg accggtgccg tcgaggcgag aagtcccgat ggccgcatcg agcgcgccga
     4321 gcaaggcgac atgatcggtg tgctcgaaac ccttggcgga ttgccgctcg acgggaccct
     4381 cgtcgccgcc agcgatggca ccgcgctgcg cgtcaaccgc agcgacctct tcgatctgtt
     4441 cgcggacgat acgaccctgg tgcaggggat tgcgtcgggc ctgctctcgg ccggcgaacg
     4501 ggtcgaggcg cggccgcgct gagcgatcag cgggtgcgtg ccggcaacgc gctcgcgtcg
     4561 ccatcgaatt cgcggcgctc cgatgactgc tgccgtccga cgcgtccggc gtggatctgc
     4621 gctcgcccgt agctctcggg cctgacgggg gagccggcca ggcggcgcag catcgccaac
     4681 tgtcccacgt gcgtcaacgc atccgcgatc ggcccctgga agatgatcgc ggccggatgt
     4741 ccgaggggcg tgtccgaggc caggtgcgcg tccagcctgg ccagggtcgc aaagaagcgc
     4801 tcgacatcga cgtcccaggt tccggcactc gccgccttcc agacccagtg tccctgcgcg
     4861 agctgctcgg cccattcgag gagatcgccg agatggccca cgatctcgag cggactacgg
     4921 gagtcaggcg cgagacgatg gtcggcgaag ccggttggcg gatcccgaag aaccttttcc
     4981 gcacgatagg ccagcgtggc cagcgtgtgc cgaagcagtg accgttcgtc cgc
//
LOCUS       DBNGSH010000467         9377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2760053, whole genome shotgun sequence.
ACCESSION   DBNGSH010000467 DBNGSH010000000
VERSION     DBNGSH010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2760053"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /gene="mraY"
                     /locus_tag="AC16CY_10890"
     CDS             <1..734
                     /gene="mraY"
                     /locus_tag="AC16CY_10890"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3354340.1"
                     /translation="TRRSHHGLIPRYKVAGQVLVATGVGLAVLYLSEQTPSLYNTRLI
                     FPFFKNLIPDLGYFYVLFAVIVLVCASNGVNLTDGLDGLAISTFGIAAAAFTALAYVT
                     GHAVLADYLLLVRFPPAGELTIFCGALVGASLGFLWYNSYPAEVFMGDVGSLALGGAI
                     GTVAILIKQELLLPIVGGVFVLEGLSVIIQVAWFKLTGKRVFKMAPLHHHFELMGWSE
                     PKVIARFIILAVIFALFALTTLKLR"
     gene            739..2106
                     /gene="murD"
                     /locus_tag="AC16CY_10895"
     CDS             739..2106
                     /gene="murD"
                     /locus_tag="AC16CY_10895"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR3354341.1"
                     /translation="MGDGDFSVRGRRVVVVGAASSGTAAAELLVRRGARVILTERRGQ
                     LDDAAKLRGAGIELELGGHREATFEAADLIVTSPGVPPEEPALATARRRGVEVIGEVE
                     LASRWLQGPILAITGTKGKSTTTTLLGRVAAASGRKVLVGGNIGVPLSSQVDGSTPDT
                     IHVVEVSSFQLETTTTFRPWIAVWLNFAADHLDRHHSVEAYAAAKARIFSSQRAGDWA
                     VVNVDDPVVMAASEGIAATRVGFSPFGSTADGFSVEGDWIVRRSATASERLVPVSAVE
                     LTGRHMLGNVVAAAAVATVAGMPRTAITGALHGFRGLEHVMEPAGRVGGVAFVNDSKA
                     TNVEAARLSIESFPRGVVALVGGRFKGGDLRDLRPALAARGRAVVALGESAHLVREAL
                     SDLLPVVEASTMREAVERGYEAARPDGVVLLAPACASFDWFRDYAERGRVFKEEVDRL
                     RKRGG"
     gene            2481..3575
                     /gene="ftsW"
                     /locus_tag="AC16CY_10900"
     CDS             2481..3575
                     /gene="ftsW"
                     /locus_tag="AC16CY_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR3354342.1"
                     /translation="MARKLKSDRILFTATALLICVSVVMVYSASAAVAFDKFGQASMF
                     LTKQALWTVLGLAGVAVAMRIDYRTYRNEAFIWSLTGVVGVLLVLVLFSTPVKGASRW
                     FNLGGLGIQPSELAKIAAILFAALTLERRMHRIDELSYSLLPIGIIVGAMSGLILLQP
                     DLGTALSLLLIVTTMVFAAGLNYRYLLGAALAILPVLYFVIVSVPYRQRRLLAFWNPW
                     ADPRDEGYQVIQSLIAVGTGGVFGRGLMGGVQKLHFLPEPHNDFIYAVIGEELGLIGA
                     TTVLICFCVIAWRGLRIAMRAPDAFGSFLALGLTTMIAVQALVNISVVLGLLPTKGIP
                     LPLVSSGGSSLLISLLGVGVLLNVSQHETG"
     gene            3641..4726
                     /gene="murG"
                     /locus_tag="AC16CY_10905"
     CDS             3641..4726
                     /gene="murG"
                     /locus_tag="AC16CY_10905"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR3354343.1"
                     /translation="MALSVLIAGGGTGGHLYPGIAVARALMARVPDAQVVFVGTAAGV
                     ESRVVPHEGFPLEVIRSAGLKGKSFRSLLRGLLLLPMSAVDAWSVLSRRRPSIVIGVG
                     GYSSGPVVALAALRGLPTLLMEQNAVPGFTNRLLAPLVSAAAVTYEDSVGVFGRKAFV
                     AGNPVRPEFLRKESHGEHKSPPGAARVLVFGGSQGAHALNMAMVEAAARLAAAAPAVG
                     VTHQSGERDLEVVRDGYRRAGLEARVEPFLFTMDREMRSADLVVCRSGATTLAELTAS
                     GRASILVPFPGATDDHQRKNAEALARAGAARMIDQRELNGDRLAAEILALLGDPAERR
                     RMGEAAARLARPDAASVIVDKALELAR"
     gene            4743..6146
                     /gene="murC"
                     /locus_tag="AC16CY_10910"
     CDS             4743..6146
                     /gene="murC"
                     /locus_tag="AC16CY_10910"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3354344.1"
                     /translation="MLGGTRRVHFVGIGGIGMSGIAELLANLGYTVTGSDVKRSEVTA
                     RLERAFGVKVFEGHAAEHVGDADVIVYSSAVRPGNLEVAAARRRGIPVIPRAEMLAEL
                     MRLRFSIAVAGSHGKTSTTAMIALVLERAGLDPTAVIGGRLAAFDGSARLGRGDYMVA
                     EADESDRSFLMLWPSIAVVTNIDDEHMESYEGLADLQQAFVDFANKVPFYGAVVACVD
                     DSNVRAVVPRITRRVTTYGLASPDAHVQGSDVELGAFGARCVVRRRTKDLLETLGPLD
                     LTVPGRHSLQNALAAVAVGDHVGLAFDRVAAALAGFHGAERRFERHGEAGGVLVVDDY
                     GHHPTEIAAVLAAARVTLGRRLVVAFQPHRYSRTAQLMDAFGPALSGADEIVLTDIYA
                     ASEEPIAGVTLERLAESIRRGSGRPVHLAPTLNDVVEAILRVARPGDAVMTLGAGSIG
                     TLPPRLLKALEPRGARS"
     gene            6143..7006
                     /locus_tag="AC16CY_10915"
     CDS             6143..7006
                     /locus_tag="AC16CY_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3354345.1"
                     /translation="MTPVAAPSDKRFRRSRVSPSRKRWRPSWRMLARVVSICLLAGLG
                     LYGAVDFVLASDALTVTRITVQGNQRMSRGEVLALLDGLTGSSLMTVELEAWRTKLRG
                     SPWVADAAIRRMFPGTLAVAISERQPMGIGRIKGALYLIDSTGAVIDEFGPNYADLDL
                     PIVDGLGAGRGADVDQARAALAGRVMSELQMWPSLAAQVSQIDVTDARNAVVMLKGDT
                     ALVRIGHERFSERLRSYLDLMPALKERIPEIDYVDLRYDARVYVRPQGSGLRNQRPQA
                     SGPRPQKAPGG"
     gene            7053..8279
                     /gene="ftsA"
                     /locus_tag="AC16CY_10920"
     CDS             7053..8279
                     /gene="ftsA"
                     /locus_tag="AC16CY_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3354346.1"
                     /translation="MTRREQYVVGLDVGTSTVICVVGESLEDGSLHIVGTGVSESRGI
                     KRGVVVNLEAAVDSIKKAIEEAELMAGIEIDHVHLALSGPHIKGFNSRGVVAVAGKNR
                     EVTRDDVRRAIDAAKAVALPSGREILHVLPQDFVVDEQDGIGAPEGMTGARLEVNVHV
                     VTGSVSSTQNIVACVNRAGVQVVDTVIEQLAASEAVLTADEKELGVALVDVGGGTVDL
                     AIYERGHLWHTAVVAVGGDHFTSDIAVGLRTPIPDAEKVKRKYGCALSSMVDEDETIE
                     VASVGGRKPRLLARRILSEILQPRAEEIFHLVWDEIRRAGYEKSLNSGIVLTGGGAIL
                     EGMPEIAEQIFDLPIRRGTPVGVAGLADHINTPVCATPVGVALYAHRNRVPETRGSGA
                     LNRVVSQLRSIFKEFF"
     gene            8332..>9377
                     /gene="ftsZ"
                     /locus_tag="AC16CY_10925"
     CDS             8332..>9377
                     /gene="ftsZ"
                     /locus_tag="AC16CY_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3354347.1"
                     /translation="MVDRHTADSLRLTLAPEQRTGARIKVIGVGGGGSNAVNRMVEVG
                     LGGVEFIVANTDVQALDHNRAGVKIQIGRKLTKGLGAGADPNIGRQAALEDTDAILQS
                     LSGADMIFVTTGLGGGTGTGGAPVIASLAAELGALTIAVVTKPFRFEGRKRALQAEAG
                     LDALREAVDTVITIPNERLLTIIDRKTSLTEAFTLADDVLRQAIQGISDLILVPGLIN
                     LDFADVKTIMSGMGVAMMGTGMAEGEGRAMQAAQRAVSSPLLEDSSVHGARGVIINVT
                     GGPDLSLSEVSEASCVIQEAAHEDANIIFGAVVDPQLTGRVKLTVIATGFDREVSRGA
                     ASETPVDLNIYTAHL"
BASE COUNT         1416 a         2986 c         3399 g         1576 t
ORIGIN      
        1 tgacgcgccg ttcccaccac ggcttgattc cgcgctacaa ggtcgccggg caggtcctcg
       61 tggccacggg agtcggcctc gcggtgctct acctctcgga gcagacgccg tcgctgtaca
      121 acacgcggct gatcttcccg ttcttcaaga acctgattcc ggatctcggg tacttctacg
      181 tgctgttcgc cgtgatcgtg ctggtgtgcg cgagcaacgg cgtcaacctg acggacggcc
      241 tggacgggct ggcgatcagc accttcggca tcgcggctgc cgcgttcacg gcgctggcct
      301 acgtgaccgg ccacgccgtg ctcgccgact acctgcttct cgtccgcttc ccgccggctg
      361 gcgagctgac gatcttctgc ggtgcgctcg tcggggcgag cctcggtttc ctctggtaca
      421 actcgtaccc cgccgaggtc ttcatgggcg acgtcggctc gctggcgctc ggcggcgcga
      481 tcgggaccgt cgcgatcctc atcaagcagg agctgctcct gccgatcgtc ggcggcgtct
      541 tcgtgctcga agggctctcg gtcatcatcc aggtcgcctg gttcaagctc acgggaaagc
      601 gcgtgttcaa gatggctccg ctgcatcacc acttcgagct gatgggatgg agcgagccca
      661 aggtgatcgc gcggttcatc atcctcgccg tgatcttcgc gctgttcgct ttgacgacat
      721 tgaagctgcg gtgatggtgt gggagatgga gacttctcgg ttcgcggacg ccgcgtggtc
      781 gtggtgggag cagccagcag cggcacggcg gcagcggaac tgctggtccg aaggggtgcg
      841 cgcgtgatcc tgaccgaacg ccgcgggcag ctcgacgacg ccgcgaaact tcgcggggcc
      901 ggcatcgagc tcgaactggg cgggcaccgg gaggcgacgt tcgaggccgc cgacctgatc
      961 gtgacgagcc ccggcgtgcc tcccgaagag ccggcgctcg ctacggcccg gcggcgcggc
     1021 gtggaagtga tcggggaagt ggagctcgcg tcgcgctggc tgcaaggtcc catcctcgcc
     1081 atcaccggga cgaaagggaa atcgaccacg acgacccttc tcgggcgggt ggcggcggcg
     1141 agcggacgga aggttctcgt cggaggcaac atcggcgtgc cgctttcatc gcaggtagac
     1201 ggatcgacgc cggacaccat tcacgtggtc gaggtcagca gctttcagct cgagaccacc
     1261 acgacattcc gcccatggat tgcggtgtgg ctcaacttcg cggccgatca tctcgaccgg
     1321 caccactcgg tcgaggccta tgcggcggcc aaggcacgca tcttctccag tcaacgggcg
     1381 ggagactggg ctgtcgtcaa tgtcgacgat ccggtggtga tggccgccag cgaaggcatc
     1441 gcggccacgc gcgttggttt ctcgccgttc ggctctacgg ccgacggctt ctcggtcgag
     1501 ggtgactgga tcgtgcgcag gagcgcgacg gcgtccgagc ggctcgttcc ggtatcagcc
     1561 gtcgagctga caggacgcca catgcttggc aacgtcgttg ccgccgctgc ggtcgcgacc
     1621 gtggcgggca tgccgaggac ggcgatcacc ggtgcgctcc acggattccg cggcctcgag
     1681 cacgtgatgg aaccggcggg ccgcgtcggc ggcgtcgcgt tcgtgaacga ctcgaaggcc
     1741 acaaacgtcg aggcggcgcg cctgtcgatc gagagttttc cgcgcggggt cgtggcgctc
     1801 gtcgggggac gcttcaaggg aggcgacctt cgcgacctgc ggcctgcgct cgccgcgcgc
     1861 ggccgcgcgg tcgtcgcatt gggtgagtcc gctcacctgg tgcgagaggc gctctccgat
     1921 ctccttccgg tcgtcgaggc gtcgacgatg cgcgaggccg tggaacgggg gtacgaggcc
     1981 gcacgacccg atggcgtcgt gctcctggcg ccggcatgcg cgagcttcga ctggttccgc
     2041 gactacgcgg agcggggacg agtgttcaag gaggaggtgg atcgtttgcg gaaacgcggc
     2101 ggctgaagcc gccgctctac aggtagagcg cacgcttcag cgtgcgcggc gaggaaacgc
     2161 ggctacaggt agagcgcacg cttcagcgtg cgcggcgagt gaggtgtgag ctgtgagcag
     2221 tcgtcagtcg gctggcgcga gtgggagcta accgctggga acgcaggcta ccccggtctg
     2281 cgaggttggt gtcccgcccc acggccgact gatgactgct gaccgcttac gcctacgctg
     2341 ctgcgcagct tcgggcgcgc ggggatcggg atcttaatcg atctcgaccc gtccagctcg
     2401 tgcaaggact ttcggtcgct gggagatggg cctgtagggg gcaagggggc agggggcagg
     2461 gggcaggggg aagctggcaa atggcgcgga agctcaaatc tgatcggatc ctcttcacgg
     2521 cgacggcgct gctcatctgc gtcagcgtcg tgatggtgta cagcgcctcg gccgccgtgg
     2581 ccttcgacaa gttcggccag gcctccatgt ttctgacgaa gcaggcgctc tggaccgtgc
     2641 tcgggctggc cggggtggcc gtcgccatgc ggatcgacta ccgcacgtac cggaacgagg
     2701 cgttcatctg gtccctcacc ggcgtcgtgg gcgtcctgct ggtgctcgtc ctgttcagca
     2761 ccccggtcaa gggcgcgagt cgatggttca acctcggggg cctcggcatt cagccgtcgg
     2821 agctcgcgaa gatcgcggcg atcctgttcg cggcgctcac gctcgagcgc cggatgcatc
     2881 gcatcgacga gctctcctat tcgctgttgc ccatcggcat catcgtcggg gcgatgagcg
     2941 gcctcatcct gcttcagccg gatctgggca ccgcactctc actgctcctc atcgtcacca
     3001 cgatggtgtt tgccgccgga ttgaactacc gctacctcct tggcgcggcg ctcgcgatct
     3061 tgcccgttct gtacttcgtc atcgtgagcg tgccctatcg ccagcgcagg ctccttgcat
     3121 tctggaatcc atgggcggat ccccgcgacg aggggtatca ggtcattcag tccctcatcg
     3181 ccgtcggcac gggcggcgtg ttcgggcgcg gcctgatggg cggcgtccag aagctccact
     3241 tcctccccga gccccacaac gatttcatct acgccgtcat cggcgaagag ctcgggttga
     3301 tcggcgccac cacggtcctc atctgtttct gcgtcatcgc gtggcgcggc ctgcgaatcg
     3361 cgatgcgggc gccggacgcg ttcggatcgt ttctcgccct cgggctcacg acgatgattg
     3421 ccgtgcaggc gctcgtgaac atcagcgtcg tgctcggctt gctgccgacc aaggggattc
     3481 cccttccgct cgtcagttcg ggcgggtcat cgctgctcat cagtctgttg ggtgtgggcg
     3541 tgctcctcaa cgtgtcgcag catgaaacgg ggtgatgcgg gtgcgagctc gacaaatgtc
     3601 ggtgcgaggg tgcaagggtg cgaaggtgcg agggtggtga gtggcactgt ccgtgttgat
     3661 cgccggtggc gggacaggag ggcatctgta tccgggcatc gccgtggcgc gggcgctcat
     3721 ggcgcgagtg cccgacgcac aggtggtgtt tgtcggaacg gcggccgggg tggagtcgcg
     3781 cgtggtgccg cacgaggggt ttccgctgga ggtcatccgc agcgcggggc tgaaggggaa
     3841 atccttccgg tcgctgctgc gcggcctgct gctcctgccg atgagcgccg tggacgcgtg
     3901 gtcggtgtta tcgcgccgcc ggccatcgat cgtgatcggc gtcggcggct acagctcggg
     3961 gccggtcgtc gcgctggccg cgttgcgcgg ccttccgacg ctgctcatgg agcagaacgc
     4021 cgtgccaggt ttcaccaacc gcctgctcgc cccgctggtg agcgcggctg ccgtcacata
     4081 tgaggacagc gtcggcgtgt tcgggagaaa ggcgtttgtc gcgggtaatc cggtccggcc
     4141 ggagtttctt cggaaggagt cacatgggga acacaagtcg ccgccagggg cggcccgggt
     4201 tctagtcttt ggcggctctc agggcgcgca cgcgctcaac atggccatgg tggaggcagc
     4261 ggcgcggctg gctgccgctg cacctgcagt gggcgtcacg catcagtcgg gagaacgcga
     4321 tttggaggtg gtcagggatg ggtaccggcg tgccgggctc gaggcccgcg tggagccgtt
     4381 cctgttcacg atggatcggg agatgaggtc ggcagacctc gtggtctgcc gatcgggggc
     4441 gacgacgctg gcggagctca cggcgtcggg acgggcgtcg atcctcgtcc cgtttccggg
     4501 ggccaccgac gaccaccagc ggaagaacgc cgaggcgctc gccagggcgg gtgcggcgcg
     4561 aatgatcgac cagcgcgagc tgaacgggga tcggctggcg gcggagatcc tggcgctgct
     4621 cggggacccg gcggagcgcc ggcgaatggg tgaggcggct gcgcgattgg cgcggccgga
     4681 cgccgcgagc gtcatcgtcg acaaggcgct ggagctggcg cggtgaatcg tacgtggcgg
     4741 tgatgctcgg cggcacccgg cgggttcatt tcgtcgggat cggcggcatc gggatgagcg
     4801 ggatcgccga gctgctggcg aacctgggct acaccgtcac cggatcggac gtgaagcggt
     4861 cggaggtgac ggcgcggctc gagcgggcgt tcggcgtcaa ggtgttcgaa ggacacgcgg
     4921 cggagcacgt cggggacgcc gacgtcatcg tgtactcctc cgccgtccgt ccgggcaacc
     4981 tggaggtcgc cgccgcgagg cggcgcggca tcccggtgat cccgagggcc gagatgctgg
     5041 ccgagctgat gcggctgcgc ttctcgatcg ccgtggcggg ctctcatggc aagacgagca
     5101 ccaccgcgat gatcgcgctg gtcctcgagc gggcagggct ggatccgacc gccgtcatcg
     5161 gcgggcggct cgcggcattc gacggcagcg cgcgtctggg tcggggcgac tacatggtgg
     5221 cagaggccga tgaaagcgac aggtcgtttc tcatgctgtg gccgtcgatc gccgtcgtga
     5281 ccaacatcga cgacgagcat atggaaagct atgagggcct cgccgatctt cagcaggcct
     5341 tcgtggactt cgccaacaag gtccccttct atggcgccgt cgtggcgtgc gtggacgaca
     5401 gcaacgtccg cgcggtcgtg ccgcggatca cccgccgcgt gacgacctat ggcctggcca
     5461 gccccgatgc gcacgtgcag ggatccgacg tcgagctcgg cgcgttcggc gcccgttgtg
     5521 tcgtgcgccg acggacgaag gacctgctgg agacgctcgg gccgctcgat ctgaccgtgc
     5581 ccggccggca cagcctgcag aatgcgctcg cggcggtcgc cgtcggcgac catgtcggcc
     5641 tcgcgttcga tcgcgtcgcc gccgcgctcg ccggctttca tggtgccgag cggcgtttcg
     5701 agcgccacgg cgaggccggc ggcgtgctcg tggttgacga ttacgggcat cacccgaccg
     5761 agatcgccgc cgttctggcg gcggcccgcg tcacgctcgg gcggcgcctc gtcgtcgcgt
     5821 tccagccgca tcgatactcc cgcacggcgc aactcatgga tgccttcggc cccgcgctga
     5881 gcggcgcgga cgagatcgtc ctcaccgaca tctacgccgc gagcgaagag ccgatcgcgg
     5941 gcgtcactct cgaacggctc gccgagtcga tccggcgcgg gtcgggccgg cccgtccacc
     6001 tggcgccgac gctgaacgac gtcgtcgagg cgatcctgcg ggttgcgcga cccggcgacg
     6061 cggtcatgac gctcggcgcg ggctcgatcg gcacgctgcc gccgcgtctg ctgaaggcgc
     6121 tcgaaccgcg gggggcgcga tcatgacgcc agtcgctgcg ccgtcggaca agcgtttcag
     6181 gcgatcccgc gtgagcccgt cgcggaagcg gtggcggccg tcgtggcgca tgcttgcccg
     6241 cgtcgtgtcg atctgcctgc tggctggcct gggcctctat ggcgcggtgg acttcgtgct
     6301 ggcgtcggac gcgctgaccg tgacgcgcat cacagtacag ggcaaccaac gcatgtcgcg
     6361 tggagaggtc ctcgcgctgc tcgacgggct gacgggatcc agcctgatga ccgtcgagct
     6421 cgaggcctgg cgcacgaagc tgcgcggctc gccgtgggtg gccgacgccg cgattcggcg
     6481 catgttcccc gggacgctgg cggtcgccat ctcggagcgg cagccgatgg gcatcgggcg
     6541 aatcaaggga gcgctgtatc tgatcgactc gacgggcgcc gtcatcgacg agttcggccc
     6601 caattacgcc gacctggatc tgccgatcgt cgacgggttg ggcgccgggc gcggcgccga
     6661 cgtcgatcag gcgcgtgcgg cgctggcggg gcgcgtgatg agcgagctgc agatgtggcc
     6721 gtcgctggcc gcgcaggtat cgcaaatcga cgtgaccgac gcgcgcaacg ccgtcgtgat
     6781 gctcaagggc gataccgcgc tcgtgcggat tggtcacgag cggttttccg agcgcctgcg
     6841 ctcgtacctc gacctcatgc cggcattgaa ggagcggatt ccggagatcg actacgtgga
     6901 tctgcggtac gacgcacgcg tgtatgtcag gcctcagggg tcagggctca ggaatcagag
     6961 gccccaggcc tcgggcccca ggcctcagaa ggccccgggc gggtaggaaa caagaggcct
     7021 gggccctggg ccctgaggcc tcgaaggagt ttatgacgcg cagagagcag tacgtggtgg
     7081 ggctcgacgt cggcacgtcg accgtcatct gcgtggtcgg cgagagcctc gaggacggca
     7141 gcctccacat cgtcggcacc ggtgtctccg agtcccgcgg catcaagcgt ggagtcgtcg
     7201 tcaacctcga agctgcggtc gattcgatca agaaggcgat cgaagaggcc gagctgatgg
     7261 ccgggatcga gatcgaccac gtgcacctgg cgctctccgg tccgcacatc aaggggttca
     7321 acagccgcgg cgtcgtggcg gtcgccggaa agaaccgcga ggtcactcga gacgacgttc
     7381 ggcgcgcgat cgacgcggcc aaggcagtgg cgcttcccag cgggcgcgag atcctgcacg
     7441 tgctgccgca ggacttcgtg gtggacgagc aggacggcat cggcgctccg gagggcatga
     7501 cgggcgcccg gctcgaggtc aacgtccacg tcgtgaccgg ctcggtgtcg tccacgcaga
     7561 acatcgtggc gtgcgtaaac cgagccggcg ttcaggtggt tgacacggtg atcgagcaac
     7621 tggcggcgtc cgaggccgtg ctgaccgccg acgagaaaga gctcggcgtc gcgctggtgg
     7681 acgtgggggg cggaacggtg gacctggcca tctacgagcg gggccacctc tggcacaccg
     7741 cagtcgtcgc ggtcggcggc gatcacttca ccagcgacat cgccgtcggc ctgcgcacgc
     7801 cgatccccga cgccgagaag gtcaagcgca agtacgggtg cgcgctttcg tcgatggtgg
     7861 acgaggacga gaccatcgag gtcgccagcg tcggcggccg caagccccgg ctgctcgcgc
     7921 gccgaatcct ctccgagatt ctgcagcccc gggcggagga gatctttcat ctggtgtggg
     7981 acgagattcg acgcgccggc tacgagaagt ccttgaactc gggcatcgtg ctgacgggcg
     8041 gcggggccat tctcgagggc atgccggaga tcgccgagca gatcttcgac ctgccgatcc
     8101 ggcgcggaac gcccgtcggg gtcgcgggtc tcgccgatca catcaacacg ccggtgtgcg
     8161 ccacgccggt cggcgtggcg ctctacgccc accgcaacag ggtgccggag acgcgcggca
     8221 gcggggcgct caaccgggtg gtgtcgcagc tgagatcgat attcaaggag tttttctaga
     8281 aatgcgcgcc ttcggcgcct cgacgaggtt cgaacctgag gaggtcttga catggttgat
     8341 cgacacacgg ctgacagcct gcggctgacg ctggcgcccg agcagagaac cggcgcccgg
     8401 atcaaggtta ttggcgtcgg cggcggcggg agcaacgccg tgaaccggat ggtggaagtc
     8461 ggcctcggcg gggtcgagtt catcgtggcc aacacggacg tccaggcgct ggaccacaac
     8521 agggccgggg tgaagattca gatcggccgc aagctcacaa aaggactcgg cgccggcgcc
     8581 gatccgaaca tcggcaggca ggcggcgctc gaggacacgg acgcgatcct ccagtcgctg
     8641 tcgggcgccg acatgatctt cgtgacgacc gggctcggcg gcggcaccgg gaccggcggc
     8701 gcccccgtca tcgcgagcct cgccgcggaa ctgggggcgc tcacaattgc cgtggtcacc
     8761 aagccgttcc ggttcgaagg aaggaagcgc gccctgcagg ccgaagccgg gctcgacgcg
     8821 cttcgcgaag cggtcgacac cgtcatcacg attcccaacg aacgcctgct gacgatcatc
     8881 gaccgcaaga cgtcgctgac cgaggccttc acgcttgccg acgacgtgct gaggcaggcg
     8941 attcagggca tctcggacct catcctggtg ccgggcctca tcaacctcga cttcgccgac
     9001 gtgaagacca tcatgtccgg catgggcgtg gcgatgatgg gcaccggcat ggccgagggc
     9061 gaaggccgag cgatgcaagc cgcgcagcga gccgtgtcga gtccgttgct cgaggacagc
     9121 tcggtccacg gcgcgcgcgg cgtcatcatc aacgtcaccg gcgggcccga cctgtcgctc
     9181 tcggaagtca gcgaagcgtc gtgcgtaatc caggaggcgg cgcacgaaga tgccaacatc
     9241 atcttcggtg ccgtcgtcga tccgcagctg acgggacgcg tgaagctcac cgtgatcgcg
     9301 accgggttcg atcgcgaggt ctcgcgcggg gccgcgtcgg agacgccggt ggacctgaac
     9361 atctacacgg cacacct
//
LOCUS       DBNGSH010000468         7814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3778686, whole genome shotgun sequence.
ACCESSION   DBNGSH010000468 DBNGSH010000000
VERSION     DBNGSH010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3778686"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="AC16CY_10930"
     CDS             complement(<1..138)
                     /locus_tag="AC16CY_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354348.1"
                     /translation="MKILVIGGGAREHAIAEALCRTGRAELLVVAQNAHPGLARLATA
                     RV"
     gene            378..575
                     /gene="rpsU"
                     /locus_tag="AC16CY_10935"
     CDS             378..575
                     /gene="rpsU"
                     /locus_tag="AC16CY_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR3354349.1"
                     /translation="MAEVKIQEGESIESALRRFKRKVQQEDIIKDIKKHSFYLKPGDK
                     RRAKQALARKRNRKKLRRETE"
     gene            611..922
                     /locus_tag="AC16CY_10940"
     CDS             611..922
                     /locus_tag="AC16CY_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HIR3354350.1"
                     /translation="MEFQDKSLKCVDCGSEFIWTAGEQLFFADKQFKNEPKRCKTCKA
                     KRANRASAGPAPARDRVETQATCSACGKETTVPFRPTQGRPVFCKECFQQRKFAGAGG
                     A"
     gene            1042..1401
                     /locus_tag="AC16CY_10945"
     CDS             1042..1401
                     /locus_tag="AC16CY_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3354351.1"
                     /translation="MQIEERSAGDVTILDLKGKMTLGEGDEALKDKINSLVLQGRKKL
                     VLNLAEVPYIDSAGLGEIVRTFTTVSRQGGSLKLLSLTKRITDLLAITKLLTVFETHD
                     NEADAIRSFQPSANVQS"
     gene            1637..2614
                     /locus_tag="AC16CY_10950"
     CDS             1637..2614
                     /locus_tag="AC16CY_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354352.1"
                     /translation="MREIRSRISERPGVDLSPQQIQELAARRLEAILDPRAIKPSLID
                     ELRRVAGLPVEPAPEAPETGTGFDATSFYDSSNGLLRIVRRLLRPLLKLFFEPDAVIQ
                     ALGAQARVNTSAAVRDAEQQRRQTEWNGLHYEILRRLVIEIARTSIDAQHLALRVESL
                     AAKVDFNERQVRGLEQTLLQSKSAGRGPELAAPPAAPPPAREGATVQERRPPDGSPEA
                     QGQRRRRRRRRGRRSGGGAPPFDVQGGGVAGLPHESRNESQEPAEEDMGGEPVGGDVE
                     HEQAAAPDSGFDEPPAEAPRTESPQHSTPTDEPAGTDVVPRNDDVAGRQ"
     gene            2611..3879
                     /locus_tag="AC16CY_10955"
     CDS             2611..3879
                     /locus_tag="AC16CY_10955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3354353.1"
                     /translation="MKLAFITPRYGADVSGGVEHACRLLAEQVARRHDVDVLTTTARD
                     PHTWHNDCTEGSDRVRGVLVRRFAVTAVDDRAGLDALSRRLREGPRSRPEELAWVRHL
                     GPWSAGLLEYIKRQHRSFDALVFFGLWHPLTVHGLEVAPERSIVFPYLQLRPELRFDL
                     WAALLSSARAVGYFSAVERPLVRSYIGVMPQYEELIGIGVDPPPRLSYPRHQQDPADV
                     ITADDDPGAETEELRDDSYLAGQGIPFRRRHRLYGDFAFYGGRVESDNGCEEMLEYFD
                     TFAAGADDMMLVLMGVKMITVPDAPYLRLAGVLPDRERMTAYEAATVTVAPGSDDLLG
                     QSLLESLAVGTPVLASARNGAAIEHLRRANAGLYYASRAEFVGAMSELRAPELRDRMG
                     HNGRRYIRQHHQWDAVLGRFERLVSRIRVQ"
     gene            complement(3883..5340)
                     /locus_tag="AC16CY_10960"
     CDS             complement(3883..5340)
                     /locus_tag="AC16CY_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3354354.1"
                     /translation="MRRTGSILVVDDEEVMREILEALLTREGYHVRLAASGEEGLDLA
                     RSVPFDAAIVDVMMPGLDGLGTLDELKKLDDELPVMMVTAFASVETAITAMKRGAFDY
                     ITKPFKHDEVLVVVRNAVERRQLMAENAALRQNLQAQYQKFAGIIGRSPRMKQVFDLI
                     IQAAPSRSTILITGESGTGKELVARAIHSNSPRAERAFVTVNSGNLPPDLLESTLFGH
                     VKGAFTGAIYPKKGLCDLADKGSIFFDEIGNIPPDTQAKLLRVMQEREFMRLGGMETI
                     KVDARIIAATNCDLRLMVGEGRFREDLYYRLHVINVYLPPLRERKDDIPYLAQHFLEK
                     YTAENNKPPLDLTADALDLLMEYDWPGNVRELENVVERAVVLSASAHIGPELIPDHVR
                     SAPMFHIPKFVVPPEGISFKDVITNVEKRLIESTLEAAGGVQKKAAELLKIKPTTLNE
                     MIKRYEIGTRRRKNGSEEAAAARSRPFPAAELSRE"
     gene            complement(5409..>7814)
                     /locus_tag="AC16CY_10965"
     CDS             complement(5409..>7814)
                     /locus_tag="AC16CY_10965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR3354355.1"
                     /translation="WVFYWGDAVSMLALPPIFLHFTRGFPERRQRWNLGPATRARLAL
                     VYLPAVVFGIARIYAVATSSGNPEAFEQQELQLDRLGFMYFALCLIGGLITLLRALSQ
                     VGTMTARRQLRWIAWGTALGAGTFAVGYAMPYALGVQPSLPMELSAVPLSLIPLAFAS
                     AIVRYRLMDIEVIVKRVLVYATALAAIVAIYAVLLEAVRQVLLKGDGSNEWVLALLVL
                     LVAVVLTPPIKDFVQNLLDRAFYRDRYDYRRALVGFARDLNSDLDLHRLAKRLVSRVV
                     ETLLVDRMTLMLEDEVAPHFGSVRSSGFGDAHPPALPKRSAIGRRLVDGHIVALDDPT
                     AVGRFALEEIEFWRDAGLSYFVPCVSKEGTIAVLAVGRKDTGDPLSSEDTGLLAAVAG
                     QIATALENARLYRQLHVKAVEVDRMRAFNENILESLDNGLLVVGLDDRVVRWNRALEQ
                     LYGVSRANASGGALDDLFDPPFVEAIRAARRDTPDGATLSRVHLVARADRAGETLVVN
                     ATVMPLLEAQGEPTAASGTIVIVEDVTARVQLEEQLRLSEKMASIGLLAAGVAHEVNT
                     PLTGISSFTQMLLEGANPQDPRTHLLEKIERQTFRAAKIVNGLLMLARPASADRSSFD
                     LNVVVTDVLGLLEHQFETHRIRVRRELSETPVHVLGLEHKLQQVFLNLFLNARDAMPK
                     GGWLSVTTRVDGQKVVAEVSDTGSGIPSEYLARIYDPFFTTKAIGQGTGLGLSITYGI
                     VREHGGSIDCESAVGQGTRFIMSFPAVSERAVQTGRSERQRPEVSRAGTRAAGHDPGR
                     TYH"
BASE COUNT         1380 a         2570 c         2565 g         1299 t
ORIGIN      
        1 gacccgcgcc gtggccagcc gagcgagacc ggggtgggca ttctgggcga ccacgagcag
       61 ctcggcgcgg cccgtgcggc agagcgcctc ggcgatggcg tgctcgcgcg cgccgccgcc
      121 gatgacgagg atcttcatga ggaacaggct aactgcctgc cggacaggag gttgaccgac
      181 cggacagggt tggatgagct cgcatccgtt ggtcaacgca ttgtgcgcag aactcaggtc
      241 cggcacacga ctatttcgtt gacgtaacgg cctcttgcgc ctagaatccg cgcctgcctg
      301 tgatagccta acatattcgc cacttttcct aggcgatcac agggagccac caggccgttg
      361 aggggggtgt tgtaggcgtg gccgaagtca agattcagga gggcgagtcg atcgaaagtg
      421 cactccgccg cttcaagcgc aaggttcagc aggaagacat catcaaggac atcaagaagc
      481 actctttcta cttgaagccg ggtgacaagc gcagagccaa acaggcgctg gcgcgcaagc
      541 ggaaccgtaa gaagctccgt cgagagacgg aatagcggtc ccgcgatcga cgagcacgag
      601 gtggcgaccg atggagttcc aagacaagag cctgaagtgc gtggactgcg gctcggaatt
      661 catctggact gcgggggagc aactcttctt cgctgacaag cagttcaaga acgagccgaa
      721 gcggtgcaag acgtgcaagg ccaaacgggc gaatcgggcc agcgcaggcc cggcgccggc
      781 gagggaccgg gtcgaaacgc aagccacgtg ctcggcttgc ggaaaagaga cgacagtccc
      841 cttccggccc acgcaaggcc gcccggtgtt ctgcaaggag tgcttccagc agcggaagtt
      901 cgcaggggct ggaggcgcct gattcagagg gaattcgcgg tcgtctccac ccaaaatcgc
      961 cgcagggcca gacgcgagtc tggccttgtt tgtctatgct agaatccgtg cttttacggt
     1021 gccaagttca ttcgagggcc tatgcagatc gaggaacgat ccgccggtga tgtgaccatc
     1081 ctggacctca agggcaagat gaccctcggc gaaggagacg aggcgctgaa ggacaagatc
     1141 aacagcctgg tgctccaggg taggaagaag ctcgtcctga atctggctga ggttccgtat
     1201 atcgacagcg ccggcctcgg agaaatcgta cgaaccttca cgaccgtcag tcgtcagggc
     1261 ggcagcctca agctgttgag cctgacaaag cggatcaccg acttgctggc aatcacgaaa
     1321 ctgctcactg tgttcgagac ccacgacaac gaagcggacg cgatccggag ctttcagccg
     1381 tcggcgaacg ttcagtcgta gggtcgcgtg ggcatcgcgc gacgtcccgc ttttcccgac
     1441 ttcatcccgg ttcgcctcgc ggagccccgc gatgaagcat cgctgatgga cgatgcctga
     1501 tcacaaccca caacgcccgc gtcgacccca ctatcaccgc ggccggcgcg gtcccgaccg
     1561 gcgcggtccc gaccggcgtc cggcgccgca gcagtccgag gcaccagcgc gagaccaact
     1621 cgacatcgag cagctcatgc gcgagattcg gtcgcgcatt tccgaacggc ccggcgtcga
     1681 tctcagccct cagcagattc aggagctcgc ggcgcgccgc ctcgaagcca ttctcgaccc
     1741 tcgcgccatc aagccgtcgc tcatcgacga attgcgccgc gtggcgggcc ttccggttga
     1801 accggccccc gaggcgccgg agacgggaac cggcttcgac gcgacctcct tttacgattc
     1861 gtcgaacgga ttgctcagga tcgtccgccg gctgttgcgc ccgttgctga aactgttctt
     1921 cgagcccgac gccgtgatcc aagcgctcgg cgcgcaagca cgcgtcaaca cgtccgcggc
     1981 cgttcgagac gcggaacaac aacgccggca gacggaatgg aacggcctgc actacgagat
     2041 cctccgtcgt ttggtcatcg agatcgcgag gaccagcatc gacgcgcagc atctcgcgct
     2101 gcgggtcgag tcgctcgctg caaaggtcga cttcaacgag cgccaggtgc gcggactcga
     2161 gcagacactg ctccagtcga agtccgccgg ccgcggcccc gagcttgcgg ctccgcctgc
     2221 cgcgccgccg ccagcgcgcg agggcgcgac cgtgcaggaa cgccggcccc ccgacggttc
     2281 gccggaggca caaggccagc ggcggcggcg gcgacggcgg cgggggcgca ggtccggagg
     2341 cggagcgcct ccattcgatg ttcaaggcgg cggagtcgcc ggtctccctc acgagagccg
     2401 caacgaatcg caggaaccag ctgaagaaga catgggcggt gagccggtcg gcggcgacgt
     2461 ggagcacgag caggccgctg cgccggattc aggcttcgac gaacctccgg ccgaggcgcc
     2521 ccgtacggaa tccccacagc actccactcc gaccgacgag ccggccggta cggacgtcgt
     2581 tccgcgaaac gatgatgtcg cgggccgtca gtgaagctgg cattcatcac ccctcgctac
     2641 ggtgccgacg tcagcggcgg cgtagagcac gcctgccgtc tgctggcgga gcaggtcgcg
     2701 cgacggcacg acgtggacgt gctgacgacc acggctcgcg atccgcacac gtggcataac
     2761 gactgtaccg agggttcgga ccgcgtccgt ggcgtgctcg ttcgtcgatt cgcggtcacc
     2821 gccgtggacg atcgcgccgg gctcgacgcc ctatcgcgcc ggctgcgcga gggtccgcga
     2881 tcgcggccgg aggaactggc gtgggtccgc cacctcgggc catggagcgc gggtcttctc
     2941 gaatacatca agcgccagca caggtccttc gatgcgctgg tgttcttcgg cctctggcat
     3001 ccgctgacgg tgcacggtct cgaggtcgcg cctgaacgaa gcatcgtgtt cccatacctg
     3061 cagttgcgtc ccgagctgcg cttcgacttg tgggccgccc tgctttcgtc ggcgcgcgcg
     3121 gtcggatact tttcggcggt cgagcgtccg ctggtccgct cgtacatcgg cgtcatgccg
     3181 cagtacgagg aactgatcgg catcggcgtc gatccgccgc ctcgcctgag ctatccgagg
     3241 caccagcagg atccggccga tgtgatcacg gcggacgacg atcccggcgc cgagacggag
     3301 gaacttcggg acgacagcta tctggcgggc cagggcatac cctttcgtcg ccgccatcgc
     3361 ctctacggag acttcgcttt ctacggaggc cgcgtcgagt ccgacaacgg gtgcgaagag
     3421 atgttggagt acttcgacac ctttgccgcg ggcgccgacg acatgatgct ggtgctgatg
     3481 ggagtgaaga tgatcacggt gccggacgcc ccgtacctcc ggctggccgg cgtcctcccc
     3541 gatcgcgagc ggatgacggc ctacgaagcc gccacggtca cggttgcgcc cgggtcggac
     3601 gacctgctcg ggcaatcgct cctcgagagc ctggccgtcg gtacgcctgt cctggcgagc
     3661 gcgcgcaacg gcgccgcgat cgaacatctc cgacgcgcca acgccgggct ctactacgcg
     3721 agccgcgcag aatttgtcgg cgcgatgagc gagcttcgag caccggagct ccgcgatcgg
     3781 atggggcaca acgggcggcg gtacattcgc cagcaccacc agtgggacgc cgttctcggc
     3841 cgcttcgagc ggctcgtctc gagaatccgg gtgcagtagg gtctattcgc gagagagttc
     3901 ggcggccgga aacggacgcg agcgcgccgc ggccgcctct tccgatccgt tcttgcggcg
     3961 ccgcgtcccg atctcgtatc gcttgatcat ctcgttcaac gtcgtcggct tgatcttcag
     4021 cagttcggcc gccttcttct ggacgccgcc ggcggcttcg agcgtcgatt cgatcagccg
     4081 cttctccacg ttggtaatga cgtccttgaa ggagattccc tctggcggga cgacgaactt
     4141 cgggatgtgg aacatgggag ccgatcggac gtggtccggt atgagctcgg gaccgatgtg
     4201 tgcgctcgcg ctcagcacga cggcacgctc cacgacgttt tccagctcgc gcacgttgcc
     4261 cggccagtcg tactccatca gcaggtccag cgcgtcggcg gtcaggtcga ggggaggctt
     4321 gttgttctcc gcggtgtact tctccagaaa gtgctgcgcc aggtacggga tgtcgtcctt
     4381 acgctcgcga agcggcggca ggtagacgtt gatgacgtgc agccggtagt acaagtcctc
     4441 gcgaaaccgc ccctctccta ccatcagacg caggtcgcaa ttggtcgcgg cgatgatcct
     4501 ggcgtcgacc ttgatcgtct ccattccccc cagccgcatg aactcgcgct cctgcatgac
     4561 tcgcagcagc tttgcctggg tgtcgggggg gatgtttccg atctcgtcga agaatatgct
     4621 ccccttgtcg gcaaggtcgc agagcccttt cttcggatag atcgcgcccg tgaacgcgcc
     4681 cttgacgtgg ccgaacaggg tggattccag gaggtcgggc ggcaggttgc ctgagttcac
     4741 cgtgacgaac gcccgctccg cgcgcggcga gttcgagtgg atcgcccgcg cgacgagctc
     4801 cttgccggtg ccgctctcgc cggtgatcag gatggtcgaa cggctcggcg cggcctggat
     4861 gatcaggtcg aagacctgct tcatgcgcgg gctccggccg atgatgccgg cgaacttctg
     4921 gtactgcgcc tggaggttct gcctgagcgc ggcgttctcc gccatgagct gccgacgctc
     4981 gacggcattg cggacgacga ccagcacctc gtcgtgcttg aacggcttgg tgatgtagtc
     5041 gaaggcgccc cgcttcatcg ccgtaatggc ggtctccacg gacgcgaacg ccgtgaccat
     5101 catcaccggc agctcgtcgt ccagcttctt cagctcgtcg agcgtgccga gaccgtcgag
     5161 acccggcatc atgacgtcca cgatcgcggc atcgaagggc accgatcgtg cgagatcgag
     5221 gccttcttcg ccgctcgccg cgagacgcac gtgatagccc tcgcgcgtga gaagcgcctc
     5281 gaggatctcg cgcatcacct cttcgtcgtc gacgacaagt attgaaccgg tgcggcgcat
     5341 ttactggtat gcctttcgcg ggttacgaag ctggttgttc gcggtactct tcctgttgac
     5401 accgtccact agtgataggt ccttcccggg tcgtgaccgg ccgctctcgt ccccgcccgg
     5461 ctgacttccg ggcgctggcg ctcggaccgg ccggtttgta ccgcccgctc gctcaccgcc
     5521 ggaaacgaca tgatgaaccg ggtaccctga ccgactgcgc tctcgcagtc gatgctgccg
     5581 ccgtgctcgc ggacgatgcc gtaggtaatc gacagtccaa ggccggtccc ctggccgatg
     5641 gctttcgtcg tgaagaacgg atcgtagatt cgcgcgaggt attcactcgg tattcccgaa
     5701 ccggtatcgg agacctccgc gaccactttc tgtccgtcga cgcgagtggt gaccgacagc
     5761 catccgcctt tcggcatcgc gtcgcgcgcg ttcaggaaca ggttcaggaa cacctgctga
     5821 agcttgtgct cgagcccgag cacgtggacg ggcgtctcgc tcagctcgcg gcgaacccgg
     5881 atgcggtggg tctcgaactg gtgctccagg agcccgagga cgtcggtgac cacgacgttg
     5941 agatcgaagg acgaacggtc ggcggacgcg ggacgcgcca gcatcagaag cccgttgacg
     6001 atcttggcgg cgcggaatgt ctgccgctcg atcttctcga gcaggtgcgt ccggggatcc
     6061 tgagggttgg cgccctccag cagcatctgc gtgaagctcg aaatcccggt cagcggcgtg
     6121 ttgacctcgt gcgcgacgcc ggctgccagc aggccgattg acgccatctt ctcggacagc
     6181 cgcagctgct cctcgagctg gacgcgggcc gtcacatcct cgacgatgac gatcgtcccg
     6241 gaagcggcgg tgggttcccc ctgcgcctcg agcagcggca tcacggtcgc gttgaccacg
     6301 agcgtctcgc cggctcgatc ggcgcgggcg accaggtgga cccgcgacag ggtggcgccg
     6361 tccggcgtgt cgcggcgggc cgcccggatg gcttcgacga acggaggatc gaagaggtcg
     6421 tccagcgcac cgccggacgc attcgcgcgg gatacgccgt acagctgctc gagcgcgcga
     6481 ttccagcgga caacgcgatc gtcgagaccg accaccagca ggccgttatc gagcgactcg
     6541 aggatgttct cgttgaacgc cctcattcga tccacttcga cggctttcac gtgcagctgg
     6601 cgatagaggc gcgcgttctc gagcgcggtt gcgatctgac cggccactgc ggcgagcagg
     6661 cccgtgtcct cgctgctgag cgggtcgccg gtgtcctttc gtccgacggc gagcacggca
     6721 atcgtgccct ccttcgacac gcaaggcacg aagtaggaca agccggcgtc gcgccagaac
     6781 tcgatctcct ccagggcgaa ccggcccacg gccgtcggat cgtcgagcgc cacaatgtgg
     6841 ccgtccacca gccgcctgcc gatcgccgat cgcttcggca gcgcgggcgg atgagcgtcg
     6901 ccgaagccgg acgaccgcac ggagccgaag tgcggggcca cctcgtcttc cagcatcagc
     6961 gtcatccgat cgaccaggag cgtctcgaca acccgggaca cgagccgctt ggcgagccgg
     7021 tgcagatcca aatcgctgtt gagatcgcgc gcaaagccaa cgagcgcgcg acgatagtcg
     7081 taccggtcgc ggtagaaggc gcggtccagg agattctgga cgaagtcctt gatcggcggc
     7141 gtcagcacga ctgcgaccag cagcaccagc agggcgagca cccactcatt cgacccgtcg
     7201 cccttgagaa gcacctgccg caccgcctcg agcagaacgg cgtagatagc gacaatggcg
     7261 gcgagcgccg tggcgtacac cagcacgcgc ttgacgatga cctcgatgtc catcaggcgg
     7321 tagcggacga tcgccgacgc gaacgccagc gggatgaggc tcagcggcac cgccgacagt
     7381 tccatcggga gcgaaggctg cacgccgagc gcgtacggca tggcgtagcc gacggcaaac
     7441 gtgccggcgc caagcgccgt tccccaggca atccaccgca gttgacggcg cgcggtcatc
     7501 gtgcccacct gcgacagcgc gcgcagcagc gtaatcaacc cgccgatgag gcacagcgcg
     7561 aagtacatga acccgagacg atccagttgc aattcctgct gctcgaacgc ctcgggattg
     7621 ccggagctcg tcgccacggc gtagatgcgc gcgattccaa acacgaccgc cggcaggtac
     7681 acgagcgcca ggcgcgcgcg cgtcgccggc ccgaggttcc atcgctgccg gcgctccggg
     7741 aatccacggg tgaaatgcag gaagatcggc ggcagcgcga gcatcgacac cgcgtcgccc
     7801 cagtagaaca ccca
//
LOCUS       DBNGSH010000469         2120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1910850, whole genome shotgun sequence.
ACCESSION   DBNGSH010000469 DBNGSH010000000
VERSION     DBNGSH010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1910850"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="AC16CY_10970"
     CDS             <1..1184
                     /locus_tag="AC16CY_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354356.1"
                     /translation="ALINSNNWPDLFDTIVRDNSSYYMLGFQPGYDRDDGRYVRLEVR
                     VARPDVHVRTRGGYLAPLREAQREPDGRNRSRERPAPTAATARLASPISTDGIPMRVF
                     ATPFKGLSDDMATVALVMELDASSLGRAVSRGESTVTLDVSYIANGAGNRDLYPEVRH
                     VARIPAAAIPAGSATARVRLFSDVELREGRYQLRVASALAGVAGSVVYDFEIPDFDDV
                     DLALSGIALLSPSETEVLTLRPDAGAGKLRPAECDASWCEPPLGPGSSALRPMGDSND
                     KPLVNGVLSRAPTAKRTFATTEDLVVVAELYEKRQRREQPHTLAVSAALRADDGRVIP
                     LSSEERPSTTTRGASGGHLFTLRLPLGAVPPGDYALQVDARSDAGQGRAVTREIPIEI
                     R"
     gene            1222..1629
                     /locus_tag="AC16CY_10975"
     CDS             1222..1629
                     /locus_tag="AC16CY_10975"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354357.1"
                     /translation="MSRHLSAAALVVSLGLLSAIPLRAHHSEAAEFDATKPVRVTGVV
                     KKVEWTNPHIWFYVEGTDEVSGRKAVWGFSGAAPNGLRRRGITKESLKVGDTVKVQGI
                     LAKDGSPNAASRGVTFADGRQVFTAAPETPVAR"
     gene            1651..>2120
                     /locus_tag="AC16CY_10980"
     CDS             1651..>2120
                     /locus_tag="AC16CY_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354358.1"
                     /translation="MRNAFDVRARVIGLLLAAGALVAVASMAGAQGAKPAPRLSNGKP
                     DFTGVWDHPRVGDITTNVKGPCAAGGRGCSNVVTGELLFTPAGKAEWDKNNKPTTYDY
                     GSHCQPWGFVRAYNTPYPHAYVHHPDRLVILWEQDNRYHMVPTDGRELPKDLEPT"
BASE COUNT          347 a          716 c          738 g          319 t
ORIGIN      
        1 ccgcgctgat caactccaac aactggcccg acctcttcga caccatcgtg cgcgataaca
       61 gctcctacta catgctcggg ttccaacccg gctacgaccg cgacgatggc cgctacgtgc
      121 ggctcgaagt gagggtcgcg cggcctgacg tgcacgtcag gactcgcggc gggtacctcg
      181 cgccgctgcg cgaggcgcag cgggagccgg acgggcgcaa tcggtcgcgc gagaggcccg
      241 cgccgactgc cgcgacggcg agactggcga gcccgatctc gacggacggc atcccgatgc
      301 gcgtgtttgc cacgccgttc aagggtctct cggacgacat ggccacggtg gcgctcgtca
      361 tggagctcga cgcttcgtcg ctcggcaggg ctgtttccag gggcgaatcg accgtcactc
      421 ttgacgtgtc gtacatcgcg aacggtgccg gcaacaggga cctctatccg gaggtgcgcc
      481 acgtcgcccg cattccggcg gcggccatcc ccgcaggaag cgcgacggcg cgcgtgcggc
      541 tcttctcgga cgtcgagttg cgcgagggcc gctatcagct gcgcgtcgcc tcggcgttgg
      601 caggagtcgc cgggagcgtc gtctacgact tcgagatccc cgacttcgac gacgtggatc
      661 tggcgttgag cgggatcgcg ctgttatcgc cgtcggaaac agaggtgctg acgctgcgcc
      721 cggatgccgg ggccggaaag ctccggccag ccgagtgcga tgcgtcgtgg tgtgagccgc
      781 cgctcggacc cggctcgtcg gccctgaggc cgatgggcga ctcaaacgac aaacccctgg
      841 tcaacggtgt gctgtcgcga gcgccgacag cgaagcgcac gtttgcaaca accgaggacc
      901 ttgtcgtcgt ggccgagctg tatgagaagc gccagcggcg cgagcagccg cacacactgg
      961 ccgtgtcggc cgcgctgcga gccgacgacg gccgggtgat ccccctgtcg tcggaagagc
     1021 gtccctcgac gacgactcgc ggcgcatcgg gcgggcacct gtttactctg cgcctgccgc
     1081 tcggtgccgt tccgccgggc gactacgcgc tgcaggtgga cgcacgctcg gacgccggcc
     1141 aggggcgcgc cgtcacgcgc gagattccca tcgagatccg gtaaagtggg acctgcgaaa
     1201 tcgaagtcag ggaggacgtt gatgtcacga catctttcag cggccgcgct cgtcgtgagc
     1261 ctcgggctcc tgagtgccat cccgcttcgc gcgcatcact ccgaggctgc ggagttcgac
     1321 gccaccaagc cggtccgcgt gacgggcgtc gtgaagaagg tggagtggac caatcctcac
     1381 atctggttct acgtcgaggg gaccgacgag gtctccggcc gcaaagccgt ctgggggttc
     1441 tcgggtgcgg cgcccaacgg actgcgccgg aggggcatca cgaaggagtc gctgaaggtg
     1501 ggcgacacgg tcaaggtgca aggcatcctg gccaaagatg gatcgcccaa cgcggccagc
     1561 cgcggcgtca cgtttgccga cggccgccag gtgttcaccg ccgcgccgga gacgccggtc
     1621 gcccgctagc ccgccggggg agagatcatc atgagaaacg cgttcgacgt ccgcgcccgc
     1681 gtcatcgggc tgctgctcgc agccggtgcg ctcgtggcgg tcgcgtcgat ggccggtgct
     1741 cagggcgcca agcccgcccc gcggctgtcg aacggcaagc ccgacttcac cggcgtctgg
     1801 gaccacccgc gcgtcggcga catcaccacg aacgtcaagg gaccgtgcgc ggctgggggc
     1861 cgcggctgct cgaacgtcgt gaccggcgag ctgctgttca cgccggcggg gaaggcggaa
     1921 tgggacaaga acaacaagcc cacgacctac gactacggat cgcactgcca gccgtgggga
     1981 ttcgtgcgcg cctacaacac gccgtacccg cacgcctacg ttcaccatcc ggatcggctc
     2041 gtgatcctgt gggagcagga caaccggtat cacatggtgc cgaccgacgg ccgtgagctg
     2101 ccgaaggatc tcgagcccac
//
LOCUS       DBNGSH010000470         3123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_552922, whole genome shotgun sequence.
ACCESSION   DBNGSH010000470 DBNGSH010000000
VERSION     DBNGSH010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_552922"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..684)
                     /gene="rdgB"
                     /locus_tag="AC16CY_10985"
     CDS             complement(85..684)
                     /gene="rdgB"
                     /locus_tag="AC16CY_10985"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR3354359.1"
                     /translation="MRRLLIATTNEGKLGEIRLLLKASPVSLVTLRDLPPVEEPEETG
                     ATFEENARLKARYYARYSGLVTVAEDSGLVIDALNGEPGVRSARFLRPEASYSERFAF
                     IFQRLAERPDRARTARFVCAVAVADNDRIIFETTGKVEGVIAAAPSGSGGFGYDPIFH
                     YPPYGTTLANVTDEAKLAVAHRGHAFRALARWLSLESSS"
     gene            complement(757..1488)
                     /gene="rph"
                     /locus_tag="AC16CY_10990"
     CDS             complement(757..1488)
                     /gene="rph"
                     /locus_tag="AC16CY_10990"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR3354360.1"
                     /translation="MRSDGRLPEQLRPTRITPNYLMHAEGSVLVEAGKTKVICTASVE
                     DRVPPFRRNTGKGWVTAEYGMLPRATTTRTQRESSSGRVGGRTQEIQRLIGRSLRAVT
                     KLEELGERTITLDCDVIQADGGTRTASISGAFVALVLAAQKMREQDMIRTMPVTDFVA
                     ATSVGIVGGEPLLDLAYAEDSKADVDMNVVKTGAGLYIELQGTAEALPFGREALNRLL
                     DLADTGVRQLIALQRGLVGQYLASK"
     gene            complement(1532..2122)
                     /locus_tag="AC16CY_10995"
     CDS             complement(1532..2122)
                     /locus_tag="AC16CY_10995"
                     /inference="COORDINATES: protein motif:HMM:NF022112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR3354361.1"
                     /translation="MSLKTWWLALFGGLAILVLIAVSAALIYGPRREAPAEPTAAGTA
                     APPSQTAHITATLFYGSEDGQALVPVRRDVPLAGNVADQGREILTAALAEQPPEPYLA
                     VIPAGTMLRAFYITDKGDAFIDLSAEVASGHPGGSLNELLTVYALVNAVTANLPAVTR
                     VQILVDGKEVDTLAGHVDLRRPLTRNEELVRKEDTK"
     gene            complement(2119..>3123)
                     /locus_tag="AC16CY_11000"
     CDS             complement(2119..>3123)
                     /locus_tag="AC16CY_11000"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR3354362.1"
                     /translation="EPGRLVVRIDADGLDLATLPGGAGLIERIRPGDQPNTVAVLLGD
                     AAGQARASVATANNVTRVVIEVPAASESRVPSPDPRVPSPDPGVPNPDPRSPIPDPRS
                     PASRAWQTIAIDPGHGGDDVGVRGARGAEEKQLTLDVARRLRAMVEARLGLRVILTRD
                     DDRALSLDERAAVANNGKANLLISLHVNGSRGPVSSGAEVFHLQLDRESAEVVRAATR
                     EGVVLPSLGGGQRRLDLVPWDLAQARYLDDSARLAEVLQEELQRQQVPMSEHPLRQAP
                     LRLLAAANMPAALVEVAYLTNPQQEARLGTDEFKAAIAQAIFTGIVRFRAWSEEPRRP
                     "
BASE COUNT          471 a         1047 c         1058 g          547 t
ORIGIN      
        1 atgtcagagg ttctcgtgcg gatcggaagg ctgcttgctg gaggtctttg ccgccgccag
       61 cggggtagcc ggcagcacca ggcgtcaact agacgattcg agcgacaacc atcgcgcgag
      121 tgcacgaaac gcatggcctc gatgggccac ggcgagcttt gcctcgtctg tcacgttggc
      181 aagcgtcgtc ccatacggcg ggtagtggaa tatcgggtcg tagccaaatc cgccgctgcc
      241 gctcggcgct gcggctatca cgccttcgac ctttcctgtc gtctcgaaga tgattctgtc
      301 gttgtccgcg acggccaccg cgcagacgaa tcgtgcggtg cgcgctcgat cgggccgctc
      361 ggccagacgc tggaagatga acgcgaagcg ctccgagtag gaggcctccg gccgcaggaa
      421 cctggccgat cgcacacccg gctcgccgtt cagcgcgtcg atgacgaggc cggagtcctc
      481 ggccaccgtc acgaggccgg agtagcgcgc gtaatagcgc gccttcagac gcgcgttctc
      541 ttcgaacgtc gcgcctgttt cctccggctc ctcgactggc ggaaggtctc gcagcgtcac
      601 gagcgagacc gggcttgcct tcaacagcaa ccggatctca ccgagcttgc cttcgttggt
      661 cgtggcgatg agcaggcgcc gcactcgttt gagtcttgag tcttgagtct tgagtcttga
      721 gttgtgagtt gtgagttgtg agtcttgagt ctcgagttac ttgctcgcca agtactgccc
      781 cacgagcccc cgctgcagcg cgatgagctg ccgcacgccc gtgtcggcga gatcgagaag
      841 acgattgagc gcctcgcgcc cgaacggcag cgcttcggcg gttccctgaa gctcgatgta
      901 caggccggcg cccgtcttga cgacgttcat gtccacgtcg gccttcgagt cctcggcgta
      961 ggcaagatcg aggagcggct ctcctccaac gatcccgacg cttgtcgcgg cgacgaaatc
     1021 ggtgacgggc atcgtgcgga tcatgtcctg ctcgcgcatc ttctgggccg ccagcaccag
     1081 cgcgacgaac gcgccggaga tcgacgcggt ccgcgtcccg ccgtcagcct gtatcacgtc
     1141 gcagtcgagc gtgatcgtgc gctcgcccag ctcttcgagc ttcgtcaccg cccgcagcga
     1201 gcggccgatg aggcgctgga tttcctgcgt gcgcccgcca accctgcctg acgaggattc
     1261 gcgctgcgtg cgcgtggtcg tcgcgcgggg cagcatcccg tactcggcgg tgacccagcc
     1321 ctttccggtg ttgcgccgga acgggggcac gcggtcctcg acgctggccg tgcagatgac
     1381 cttcgtcttg ccggcttcga cgagcacgga gccctcggcg tgcatcaggt agttgggagt
     1441 gattcgtgtc gggcgaagtt gttcgggaag tctgccgtcg gatctcattg gagaacactg
     1501 taattcaaaa ctcaaaactc aaaactcaaa actacttcgt gtcctccttc ctgacgagct
     1561 cttcgttcct cgtgagcggc cggcgcaggt cgacgtgacc ggccagcgtg tcaacctcct
     1621 ttccgtcaac gagtatctgc acgcgcgtca ccgccggcag gttcgccgtg acggcgttga
     1681 cgagcgcgta caccgtcagc agctcgttca gcgagccgcc cggatggccg ctggcgacct
     1741 cggcgctcag atcgatgaac gcatccccct tgtcggtgat gtagaaggcc cgcagcatcg
     1801 tgccggcggg aatgacggcc aggtacggct cgggcggctg ttcggcgagc gccgccgtca
     1861 ggatctcgcg tccctggtca gcgacgttgc cggccagcgg cacatcgcgc cgaaccggta
     1921 cgagcgcctg gccgtcctcc gagccgtaga acaacgtcgc cgtgatgtgc gctgtctgcg
     1981 acggcggggc agccgtgcct gcggcggtcg gctcggccgg agcttcgcgg cgtggaccgt
     2041 agatgagcgc ggcgctcacg gcgatgagca ccaggatcgc cagccctcca aagagggcca
     2101 gccaccaggt tttcaggctc atggcctgcg cggctcctcg gaccaggcgc ggaaccgcac
     2161 gatgccggtg aagatcgcct gcgcgatcgc cgccttgaac tcgtcggtgc ccaggcgggc
     2221 ttcctgttgc ggattggtga ggtaggcgac ctcgacgagc gccgccggca tgttggccgc
     2281 ggcaagcagg cgcagcgggg cctgcctgag cgggtgctcg ctcatcggca cctgttgccg
     2341 ctgcaactcc tcctgcagaa cctccgccag gcgcgccgag tcgtccaggt atcgcgcctg
     2401 cgccaggtcc cacgggacga ggtccaggcg acgctgcccg ccaccgagcg acggcaggac
     2461 gacgccttca cgcgtcgcgg cgcggaccac ttccgcgctc tcgcgatcca gctgcaggtg
     2521 aaacacctcc gcgccggacg acaccgggcc gcgggagccg ttgacgtgca ggctgatcag
     2581 caggttggcc ttcccgttgt tcgccacggc cgcccgttcg tccaggctca gcgcgcggtc
     2641 gtcgtcgcgc gtgaggatca cgcgcagccc gagccgggcc tcgaccatcg cgcgcagccg
     2701 gcgcgccacg tcgagcgtga gctgtttctc ctcggcgccc cgagcgccgc ggacccccac
     2761 gtcgtctccg ccatgaccag gatcgatcgc gatcgtctgc catgcgcgcg aggcggggga
     2821 tcggggatca gggattgggg atcggggatc gggattcggg actccggggt cgggactcgg
     2881 gactcgggga tcgggactcg ggacgcgcga ctcgctggcg gcgggtactt cgatgaccac
     2941 gcgcgtcacg ttgttcgccg tggcgacgct tgcgcgagct tgtcccgcgg cgtcgccgag
     3001 gagcacggcg accgtgttgg gttgatctcc agggcggata cgctcgatga gaccggcgcc
     3061 ccccgggagc gtcgcgagat cgagcccatc ggcatcgatg cggacgacca gccgcccggg
     3121 ctc
//
LOCUS       DBNGSH010000471         1905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2760173, whole genome shotgun sequence.
ACCESSION   DBNGSH010000471 DBNGSH010000000
VERSION     DBNGSH010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2760173"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1905
                     /locus_tag="AC16CY_11005"
     CDS             <1..>1905
                     /locus_tag="AC16CY_11005"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3354363.1"
                     /translation="GLSTGSAFNGGGVSGYIIDTANSQEMQLTLSGGLGESEVGGTIV
                     NFVPKTGGNTFSGTFFGSTAGEWSQGNNIDADLEAKGLSQPAALYKNWDAQASVGGPI
                     FRDRLWFLGNYRDFGTHEDILGMYGNLNAGNPSSWRYEADPNLKSRNAVSRTVTSLRL
                     TAQITPRNKVGFFFDNQKACDGSAMIADAGNCRSAGEGWVASGLTAGIFPYAPEAASG
                     AQGSQGGAFGYADSWQRVWQATWTAPVTSRLLLEAGASTYISKWGWMEQPGAILHLNQ
                     VQEQTSQTGVFADGTRWTMPANMFYRGLDWNFNNMQNPTTWRAAASYVTGAHSFKFGY
                     IAAYNRTDNQNHYNDTRVNYRFSGGLPNQLTMNLGEWLIMDRSQYHAFYAQDQWTIDR
                     LTLQGAVRYDRAWSWSPEGQGATGTDRFRATPITFPYTEGVPGFNDITTRVGGAYDVF
                     GNGKTSLKVNLGKYLQSANNQDRYTLMNPAQGTRFQRTTNRTWNDRAGLGVDNDYIPQ
                     CDYMNPLANGECGPWQSPSFGQALSAAPINPEILHGWGVRPSDWQLGISVQQEVLPRT
                     SVEVGYHRRWFDGFTITDNRASTAADYTEYTFTAPVHPQLPGGGGYPVTSLNPRTNFS
                     AANNYTTFAS"
BASE COUNT          367 a          623 c          629 g          286 t
ORIGIN      
        1 cggcctgagc acgggctccg cgttcaatgg cggcggcgtg tcggggtaca tcatcgacac
       61 cgcgaactcg caggaaatgc agctgacgct ctcgggcggc ctcggcgaga gcgaggttgg
      121 cggcaccatc gtcaacttcg tcccgaagac cggcggcaac acgttctccg gcacgttctt
      181 cggcagcact gccggggaat ggtcgcaggg caacaacatc gacgcggacc tggaggcgaa
      241 ggggctgagc cagccggcgg cgctgtacaa gaactgggac gcgcaggcct cggttggcgg
      301 cccgatcttc agggacaggc tgtggttcct cggcaattac cgggacttcg gcactcacga
      361 ggacatcctc gggatgtacg gcaacctgaa cgccggcaat ccgagcagct ggcgctacga
      421 agccgatcca aacctcaagt cgcgcaacgc ggtctcgcgc acggtcacgt cgttacggct
      481 gactgcgcag atcactccgc gcaacaaggt cgggttcttc ttcgacaacc agaaggcgtg
      541 cgacggctcg gccatgatcg ctgatgcggg caattgccga tccgcgggcg agggctgggt
      601 ggccagcggc ctcacggccg ggatctttcc gtacgcgccg gaggcggcca gcggtgcgca
      661 aggcagccag ggcggcgcat tcgggtacgc cgactcgtgg cagcgcgtgt ggcaggccac
      721 gtggacggcg ccagtcacga gccgcctgct gctcgaggcg ggtgcctcga cctacatcag
      781 caaatgggga tggatggagc agccgggcgc gatcctgcat ttgaaccagg tgcaggagca
      841 gacgtcacag actggcgtct tcgcagacgg cacgcggtgg acgatgccgg ccaatatgtt
      901 ctaccggggg ctcgactgga acttcaacaa catgcagaac ccgacgacgt ggagggcggc
      961 ggcgtcgtac gtgaccggtg cgcacagctt caagttcggg tacatcgcgg cgtacaaccg
     1021 gaccgacaac cagaaccact acaacgacac gcgcgtcaac taccggttct caggcggcct
     1081 cccgaaccag ctcacgatga accttggcga gtggctgatc atggaccgca gccagtatca
     1141 cgcgttctac gcgcaggatc agtggacgat cgaccgtctg accctgcagg gcgccgtgcg
     1201 ctacgaccgc gcgtggagct ggtcgccgga agggcagggt gcgacaggca ccgatcggtt
     1261 ccgcgcgacc cccatcacgt tcccgtacac cgagggcgtg cccggcttca acgacatcac
     1321 cacgcgcgtg ggaggcgcct acgacgtgtt cggcaacggg aagacgtcgt tgaaggtgaa
     1381 cctcgggaag tacctgcaga gcgccaacaa ccaggaccgg tacacgttga tgaacccggc
     1441 ccagggcacg cgcttccagc gcaccacgaa ccggacctgg aacgatcgcg ccggcctcgg
     1501 ggtcgacaac gactacatcc cgcagtgcga ttacatgaac ccgctcgcca acggcgaatg
     1561 cggcccgtgg cagagtccgt cgttcggcca ggcgctctcg gcggctccga tcaacccgga
     1621 gatcctccac ggctggggcg tccgcccgtc cgactggcag ctcggcatct cggtgcagca
     1681 ggaggtcctg ccgaggacgt cggtggaggt cggttaccac cgccggtggt tcgacggttt
     1741 cacgatcact gataaccgcg cctcaaccgc tgccgattac acggagtaca cgttcacggc
     1801 gccggtgcac ccgcagcttc caggcggagg cggatacccg gtgacgtcgc tcaatcctcg
     1861 gacgaacttc tccgccgcca acaactacac gacgtttgcc agcga
//
LOCUS       DBNGSH010000472         1652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2420617, whole genome shotgun sequence.
ACCESSION   DBNGSH010000472 DBNGSH010000000
VERSION     DBNGSH010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2420617"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..947
                     /locus_tag="AC16CY_11010"
     CDS             78..947
                     /locus_tag="AC16CY_11010"
                     /inference="COORDINATES: protein motif:HMM:NF052887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8021 domain-containing protein"
                     /protein_id="HIR3354364.1"
                     /translation="MTRYIPALLVAIAAIASPARANAQAPCDRACLIGFISQYVGALV
                     ARDSSKLPLAPGVKFTEDTKVMKVGEGLWQTASGLRGYRQDIIDVRQGVAGAYLVVEE
                     KGSPVELVLRLRIVNRRITEIETVVVRSAAEGMIFAVDQLKAANPAMTLRPAPSSLNT
                     REAAIRIAMTYPEGLRIGSFVKANTPFAPDAYRFENGRLMAGKECTFIPGCNDIKEQR
                     IPTLAEITARVAAVDEDMGIVWLRMDFGRGSVKDPESSLTVWEAFKIYGAEIHAVEAF
                     MEVMPRGASSGWD"
     gene            complement(1117..>1652)
                     /locus_tag="AC16CY_11015"
     CDS             complement(1117..>1652)
                     /locus_tag="AC16CY_11015"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3354365.1"
                     /translation="LDRYGQITRRLFEHLNTRFYQSSGMSKFVSLIYGEISDDAKFRF
                     LSAGQPYPSVFSRHHDRFMEVSPHLCLSFPPLGMLPSFNVIDRSTTPSLLGFKERYEM
                     NEWTLMGRGDILLLHTDGLTEHEGPAGRYFPARLEECLRRVKHLDARQIYDAINADLV
                     EFAPPIDDVSVVVIKVT"
BASE COUNT          321 a          505 c          530 g          296 t
ORIGIN      
        1 atgacacggt actccgccgt atgacacggt actccaacgc cgtagagcgg aggcttcagc
       61 ctccgcggag acagcgtatg acgcggtaca ttccggcgtt gctcgtggcg atcgccgcga
      121 tcgcttcgcc tgcgcgagcg aatgcgcagg cgccctgcga ccgggcctgt ctgatcggct
      181 tcatctcgca gtacgtcggc gcgctcgtcg ctcgcgactc gagcaagctg ccgctggcgc
      241 ccggcgtgaa gttcaccgag gacacgaagg tcatgaaggt gggcgaaggg ctctggcaga
      301 cggcgtccgg cctgaggggc taccgccagg acatcatcga cgtccgtcag ggcgtcgccg
      361 gcgcctacct cgtggtcgaa gaaaaagggt cgcctgtgga actcgtgctc cggctcagga
      421 tcgtgaatcg gcgcatcacc gagatcgaga ccgtggtcgt gcgaagcgcc gccgagggca
      481 tgatcttcgc cgtcgaccag ctcaaggcgg cgaacccggc catgacgctg aggccggctc
      541 cctcgtcgtt gaacacgcgc gaggccgcca tccgcatcgc gatgacgtac cccgaggggt
      601 tgaggatcgg cagtttcgtg aaggccaata cgcccttcgc ccccgatgcc tatcgcttcg
      661 agaacggccg gctgatggca gggaaggaat gcacgttcat cccgggctgc aacgacatca
      721 aggaacagcg cattcccacg ctcgccgaga tcacggcgcg cgtcgctgcc gtggacgagg
      781 acatgggcat cgtctggctg cgcatggact tcggccgcgg ctcggtcaag gatcctgagt
      841 cgtcgctgac cgtgtgggag gccttcaaga tctacggcgc cgagattcac gccgtcgagg
      901 cgttcatgga agtgatgccc cggggcgcct cttccggctg ggattaatgg acatttgtga
      961 ttcttgatta gtgattgttg aatcgttgtt gattgaagat tgttgattgg cgattgggca
     1021 cccaaccagc gatcaatcac caataaatca ccaatctcga aacaccaatc aacaacgaat
     1081 caagaatcaa caatcaccaa tcccaaatcg acaagttcac gtgactttga tgacgaccac
     1141 gctcacgtcg tcgattggcg gcgcgaactc cacgaggtcc gcgttgatcg cgtcgtagat
     1201 ctgccgcgcg tccagatgct tgacgcgacg caggcactcc tcgagacgag ccgggaagta
     1261 ccggccggcc ggtccctcgt gctcggtgag gccgtcggtg tgcagcagca ggatgtcgcc
     1321 gcgccccatc agcgtccact cgttcatctc gtaccgctcc ttgaacccca gcaggctcgg
     1381 cgtcgtgctg cggtcgatga cgttgaagga cgggagcatg ccgagcggcg gaaacgagag
     1441 gcacaggtgc gggctgacct ccatgaaccg gtcgtgatgc cgcgagaaca ccgacgggta
     1501 cggctgcccc gcggagagga agcggaactt cgcgtcgtcg gagatctctc cgtagatgag
     1561 cgagacgaac ttgctcatcc ccgacgactg gtagaaccgg gtgttgaggt gctcgaacag
     1621 gcggcgcgtg atctggccgt agcgatccag ct
//
LOCUS       DBNGSH010000473         2492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3439245, whole genome shotgun sequence.
ACCESSION   DBNGSH010000473 DBNGSH010000000
VERSION     DBNGSH010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3439245"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /gene="sppA"
                     /locus_tag="AC16CY_11020"
     CDS             complement(<1..1652)
                     /gene="sppA"
                     /locus_tag="AC16CY_11020"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR3354366.1"
                     /translation="MAVRRGIGLVLALIGLAVIVSLASLALMFVVMSSAPSVPSSATL
                     VLRPAGDLAEMQPDNVVGQVLGREVATVRGFVESLRKARRDPRVRTVLLSPSILELPY
                     WGKVQELRDAVLDFRKSGKTVVAFLEYGGEREYYLASAADRVFLMPTSLLDLTGVASY
                     EIFLRGTLDKLGAYPDFVHTGDYKTAANQLTERGFTPEHREMTQSLNREMYNQLVKGI
                     AEGRKKTEAEVRALLDQGPLVAKQALEMGLVDELAYEDQLADLVPELADDDRSRIDGL
                     DYQRVKPESVGFRPRSRIAVLYAVGAIASGRSTYDVLNGPVVGSDTLVEEIKKIREDD
                     SIKAIVVRIDSPGGSAVASDVIWRELMITREDNPSRPIVTSMSDLAASGGYYIAIPGE
                     VIVAQPGTLTGSIGVIVGKIVIGGSLDKIGAAGEEVINGVNAGIYSPFERFTPGQRTR
                     IEAYMNDVYDGFVMRVAESRKATPEKIRAVAGGRVWTGRQAMEHGLVDALGGLDTAIA
                     IAKERARIPEDEDVQLVVYPARRSLYDALSQEFGGVSGVGLWA"
     gene            1822..2184
                     /locus_tag="AC16CY_11025"
     CDS             1822..2184
                     /locus_tag="AC16CY_11025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3354367.1"
                     /translation="MGQSNITELVKTDNVTGSGNEAVAGRQVTVHYTGWLYDQARADH
                     KGKQFDSSRSSGQPFQFRLGAGRVIQGWDEGVAGMKVGGRRTLTIPPHLGYGSQGFGA
                     DIPPNSTLLFEVELLDVR"
     gene            complement(2240..>2492)
                     /locus_tag="AC16CY_11030"
     CDS             complement(2240..>2492)
                     /locus_tag="AC16CY_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354368.1"
                     /translation="LPRTTSARSPAFCAGTEMSARRLNSIRGRPEHRNEYSDHRAGTH
                     RPGTNAHALPGDGEWNGHHAPSVTGNAVTRGVERFNLEV"
BASE COUNT          425 a          829 c          795 g          443 t
ORIGIN      
        1 gcccacaagc ccacgccgct gacgccgccg aactcctgag aaagcgcgtc gtacaggctt
       61 cggcgcgccg gatagacgac gagctgcacg tcctcgtcct ccggaatgcg ggcgcgctcc
      121 ttggcgatcg cgatcgccgt gtcgagcccg ccgagggcgt caaccagccc gtgctccatc
      181 gcctgccgtc ccgtccacac gcgaccgccc gccacggcgc ggatcttttc gggcgtggcc
      241 tttcgagact cggcgactct catgacgaag ccgtcataga cgtcgttcat gtaggcctcg
      301 atcctggtcc gttgccccgg cgtgaacctc tcgaagggcg agtagatgcc ggcgttgact
      361 ccgttgatca cttcctcgcc cgccgccccg atcttgtcga gcgacccgcc gatcacgatc
      421 ttgcccacga tcacgccgat cgagccggtg agcgttccgg gctgcgcgac gatgacctcg
      481 cccgggatcg cgatgtagta cccgcctgac gccgccaggt ctgacatcga ggtgacgatc
      541 ggcctcgacg ggttgtcctc gcgcgtgatc atgagctcgc gccagatgac gtcggacgcc
      601 accgccgatc cgccagggct gtcgattcga accacgattg ccttgatcga gtcgtcttcg
      661 cggattttct tgatctcctc cacgagcgtg tcggagccga cgaccggccc gttcaggacg
      721 tcgtaggtgc tcctgcctga cgcgatggcc ccgacggcgt acagaacggc aatccgcgat
      781 cgagggcgga aaccgaccga ctcgggcttg acacgctggt agtccaggcc gtcaatgcgc
      841 gaccggtcat cgtcggcaag ctccggcacg aggtcggcga gctggtcctc gtacgcaagc
      901 tcgtcgacga gccccatctc gagcgcctgc ttcgccacga ggggcccctg gtcgaggagg
      961 gcgcggacct ccgcttccgt tttcttccgg ccctcggcga tccctttcac gagctggttg
     1021 tacatctcgc gattgagcga ctgcgtcatc tcgcgatgct ccggggtgaa ccctctctcg
     1081 gtcaactgat tggccgccgt cttgtaatcg ccggtgtgga cgaaatcagg ataggcgccg
     1141 agcttgtcca gggtgccgcg caggaagatc tcgtaggagg caaccccggt cagatcgagc
     1201 aggctggtcg gcatgaggaa cacacggtcg gccgcgctgg caaggtagta ctcgcgctcg
     1261 cctccgtatt cgagaaacgc gacgacggtc tttcccgact tccggaagtc gaggaccgcg
     1321 tcgcgaagct cctggacctt gccccaatac ggaagctcca ggatcgacgg cgacaggagg
     1381 accgttcgga cccgcgggtc gcgtctggcc ttgcggagcg attcaacgaa tccacgcacc
     1441 gtggccacct cccgtccgag cacctgcccg acgacgttgt cgggctgcat ctcggcgagg
     1501 tcgccggcgg gccgcagtac gagcgtcgcg gatgatggca ccgatggcgc gctcgacatg
     1561 acgacgaaca tcagcgcgag gctggccagc gacacgatga ccgccaatcc gatgagtgcc
     1621 aacacgagac cgataccccg tcgtacagcc acgatgccct cctggaaggt gagatgaatt
     1681 ccattgtacg gcttcgggct tcgtgcttcg ggctccaagc accggccgca gggtctataa
     1741 tcgcgagccg tgtcgaaatt ctggacggtc atcccacttg tcctgctcgc agcgtatggc
     1801 tgtcagtcgg agcccgcgaa catgggtcaa tcaaacatca ccgagctcgt caagaccgac
     1861 aacgtgacag gcagcggcaa cgaagcggtc gccggacgcc aagtcaccgt gcactacaca
     1921 ggctggctgt acgaccaggc acgcgcggac cacaagggga agcagttcga cagctcgcgc
     1981 agctccggtc agccatttca gtttcgcctc ggggccggac gggtcattca agggtgggac
     2041 gagggcgtgg ccggcatgaa ggttggcggc cgccgtacgc tcaccattcc accgcatctc
     2101 ggctacggca gccagggctt cggcgccgac attccaccga actcgacgct gttgttcgag
     2161 gtcgagctgc tggacgtgag atagccacga agatcacgaa gaacagccac gtgatcgtcg
     2221 tgccttcgtg gctgacttct tagacctcga gattgaatcg ctcgacgccg cgggtgacgg
     2281 cgttgcccgt cacggacggc gcgtgatgac cgttccattc accatccccg gggagcgcgt
     2341 gcgcgttcgt cccgggccgg tgcgtcccgg cgcgatggtc gctgtactcg ttgcggtgct
     2401 ccgggcgtcc ccgtatcgag ttcaggcggc gtgcgctcat ttcggtcccg gcgcagaagg
     2461 cgggcgaacg tgcggaggtt gttcgtggca gc
//
LOCUS       DBNGSH010000474         1584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3778989, whole genome shotgun sequence.
ACCESSION   DBNGSH010000474 DBNGSH010000000
VERSION     DBNGSH010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3778989"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="AC16CY_11035"
     CDS             <1..440
                     /locus_tag="AC16CY_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3354369.1"
                     /translation="LVSRFEQGRITRRELIQAIAALAALSTTASAAAPLRAGSINHVS
                     VLVSDLQRSIDFYNRVFSLSVISEDKANKIVRLGIDKKILVSIRLEPPPGLVDHFAIG
                     VENFNRDAVTRDLKEMGLTPQENIEFGFHVKDPDGANVQITGI"
     gene            complement(452..1240)
                     /locus_tag="AC16CY_11040"
     CDS             complement(452..1240)
                     /locus_tag="AC16CY_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3354370.1"
                     /translation="MDRRIRLVAGAWTLMAAALAAQRDGPQFEVVSIKLSPPDAPGGQ
                     AGLRPGGRYAMTNGPVRILIGAAYPSQTNELVGAPDWVTYERYSVTAVAGRSVSEDEF
                     RAMMRNMLAERFRLSARYDSVDRPVYALVRARDDGTLGPRMRRTTANCDSAPSEPPPP
                     TGPVPPCTARFTNGSIVATGFSMSALAANLARAAGRTVVDRTGLTDSFDFSLEYATDP
                     LAGGAGDAPSLFTALEEQLGLRLQASRAVIPVVVIDHIERPTPD"
     gene            1368..>1584
                     /locus_tag="AC16CY_11045"
     CDS             1368..>1584
                     /locus_tag="AC16CY_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354371.1"
                     /translation="MRALGLLALFLGVVAPLGAQTVQNPITPPPVARAVNLSGPRIGF
                     TALSDGVVEKLHERLIDVRPTISQFGWQ"
BASE COUNT          279 a          527 c          511 g          267 t
ORIGIN      
        1 aactggtgag ccgcttcgag cagggccgga tcacgcggcg agagctgatt caggcgatcg
       61 cggccctcgc ggccctgagc acgacggcct cggccgctgc ccctcttcgc gccggcagca
      121 tcaatcacgt ctcggtgctc gtcagcgacc tgcagcgctc gatcgacttc tacaaccgcg
      181 tgttcagcct gtcggtcatc agcgaggaca aggccaacaa gatcgtcagg ctgggaatcg
      241 acaagaagat tctcgtctcg atccgcctgg agccgccgcc cgggctcgtc gaccacttcg
      301 ccatcggggt ggagaacttc aaccgggacg cggtcacgcg cgatctgaag gagatggggc
      361 tgacaccgca ggagaacatc gagttcgggt tccatgtaaa ggatcccgac ggcgcaaacg
      421 tgcaaatcac cgggatctag ccgcgtaccc atcaatcggg cgtgggacgt tcgatgtgat
      481 cgatgacaac gacggggatg accgcgcgcg aggcctgaag cctcagaccg agctgctcct
      541 ccagggccgt gaacagcgaa ggggcgtcac cggcaccacc cgccagcggg tccgtcgcat
      601 attcgagaga gaagtcgaaa gagtccgtca gcccggttcg atccaccacc gttctccccg
      661 ccgcgcgcgc cagattcgcc gccagcgctg acatcgagaa accagtcgcg acgatcgatc
      721 cgttcgtgaa tcgggctgtg cacggcggga cgggcccggt cggcggcggg ggttcggatg
      781 gcgccgagtc gcaattcgcc gttgtgcggc gcatccgggg tccgagcgtc ccgtcgtcgc
      841 gcgcgcgcac gagagcgtac accggtcgat cgacgctgtc gtatcgcgcc gacaggcgaa
      901 accgttcggc gagcatgttc cgcatcatcg cgcggaactc gtcttcgctc acgctccggc
      961 ccgcgacagc ggtgacgctg tagcgttcgt aggtcaccca gtctggagca ccgacgagtt
     1021 cgttcgtctg gcttggatac gccgcgccaa tcaggatgcg gaccgggccg tttgtcattg
     1081 cgtaacgccc gccgggccgc agtccagcct gtccgcccgg cgcatccggc gggctcagct
     1141 tgatcgagac cacttcgaat tgcggcccat ctcgctgcgc cgcaagggcc gcggccatca
     1201 acgtccacgc gccggcgacc agtcgaatcc ggcggtccat gcggttcagg gtagcactgt
     1261 gccagcagtt cctgaatcgt gcaggaccag agcgatcagg gtttttcagg aactccgtca
     1321 agacatccaa tggtgcccgc gcacaccatg cctcatggag gtgacgtatg cgggcactcg
     1381 gtcttctcgc gctgttcctc ggtgtcgtgg cgccgctcgg cgcgcagacg gttcagaacc
     1441 ctatcacccc gccgcccgtg gcgagggcgg tcaacctgtc gggtccccgg atcggcttca
     1501 cagccctcag cgacggcgtc gtcgagaaac tgcacgagcg gttgattgac gttcggccga
     1561 ccatcagcca gttcggatgg cagt
//
LOCUS       DBNGSH010000475         3343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2760595, whole genome shotgun sequence.
ACCESSION   DBNGSH010000475 DBNGSH010000000
VERSION     DBNGSH010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2760595"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="AC16CY_11050"
     CDS             complement(<1..479)
                     /locus_tag="AC16CY_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354372.1"
                     /translation="MTATSPLIRVRPLSFDSRLVFRVYMWTTLVTGCAIVGTGIRGIP
                     GSPHPRSSLIWIGGALLIAASSAASGLSLINDAADRRRALYRFGAGHLLLGLVAWMQW
                     ALLWDERGVPLLPVLIPIVAGIPFIGAALAMSIAAEETSPSPTLHSRASYDEHILQIA
                     "
     gene            complement(609..998)
                     /locus_tag="AC16CY_11055"
     CDS             complement(609..998)
                     /locus_tag="AC16CY_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3354373.1"
                     /translation="MVVQAGAIAVRQRGKEPEVVLVRSRRTGDWIFPKGHIEEGESAD
                     ETAVRELEEEAGVTGEALTVVGSSRFRLDGEQFEVTYYLVRSLSSQRSPEMREVKWCR
                     FDEARTLLEFADARRLLDKAEKLIPEV"
     gene            1046..1783
                     /locus_tag="AC16CY_11060"
     CDS             1046..1783
                     /locus_tag="AC16CY_11060"
                     /inference="COORDINATES: protein motif:HMM:NF016430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3354374.1"
                     /translation="MSDDSPRRDAFPTTRISLILAARGESEPVAREALAVLCQSYWYP
                     IYAYLRRLGHTQDAAEDLTQGFFARVLEKHYLDDFERQRGRFRSYLLTALKHYVANER
                     DRDHAQKRGGAVATLSLDTVLAAAETRYRLEPSTELTPERIFEQQWALAVLARVQERL
                     AGEEADGGRGERYDRLKVFLTGEDSDVRYRTLAGELKTTEGAIKVLVHRLRRRFKDLL
                     REEISHTVAHADDVGDELRHLLTALRG"
     gene            1965..>3343
                     /locus_tag="AC16CY_11065"
     CDS             1965..>3343
                     /locus_tag="AC16CY_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3354375.1"
                     /translation="MTDANPPEATRCRNCGTEFSGRSTPLGLCPACLIKLGMSDPNWT
                     PPALPPETPLDTPAAAASAATMVASRRFPRLPRLPVSVWIAAGALLALLFVLSQFLMA
                     PGPGYNTPSGPVVRFTIALPDDVELADGAQFAVSPDGRQIVAVARDPRGRQQLWVRSF
                     ASPEWRELRQSDGASHPFWSPDSRQVGFFADRRLKRTDVASGLTSVVCDAPSGRGGAW
                     TAGGVIVFAPGSGPLMRVPAAGGTPEQVTTVDEPRGALAHAWPGVLPDPQRFVYSASG
                     GIGEKNESPTFARSMDTGESRVIVQDGWSAAFARGYLFFVQGASLIAQPFDARRLEAT
                     GDVRRVPGADEVGGAPSEGAAFSVSDDVLAFRTGRPSLSHLVWFDRRGEAVADAQEPG
                     DFQQFSISPDGTRVAIARRDERDGSSNIWALELQRGTNSRVTLGRARDSSPVWSPDAT
                     RIAFASDRG"
BASE COUNT          515 a         1172 c         1132 g          524 t
ORIGIN      
        1 gcgatctgca ggatgtgctc gtcatagctc gcacgcgagt gcagcgtcgg cgacggcgat
       61 gtctcctcgg ctgcgatcga catcgcgagt gccgcgccga taaaagggat gcccgcgacg
      121 atcgggatca gcaccggcaa cagcggcacg ccgcgctcgt cccagagcag tgcccactgc
      181 atccaggcga ccaggccgag cagcagatgt ccagcgccga accggtaaag agcgcggcgg
      241 cggtccgcgg cgtcgttgat gagtgacagc ccggatgcgg cgctgctcgc cgcgatcagc
      301 agggcacccc caatccagat caacgacgag cgcgggtgcg gcgaccccgg tatcccgcgg
      361 atacccgtgc ccacgattgc gcagccggtg acgagggtgg tccacatgta gacccggaac
      421 accagcctcg aatcgaagga gagcggccgg acgcgaatga gaggagatgt ggcggtcatg
      481 gcaagagtct aggccgtcag cgacgggttc acatccctcg gctggtggca tccgtatccc
      541 ccattcgggg gaaaggcatc gttcggggaa aggcatcgta gagcgggggc ttcagccccc
      601 gcggacacct acacctcggg gatcagcttc tctgctttgt ccaggaggcg tcgtgcgtcc
      661 gcgaactcga gcagcgtgcg cgcctcgtcg aagcggcacc acttgacctc gcgcatttcg
      721 ggcgagcgct gcgacgacag cgaccgaacc aggtaatacg tcacctcgaa ctgctcgccg
      781 tccaggcgga accgcgacga gccgacgacc gtcagcgctt cgccggtgac gcctgcttcc
      841 tcctccagct cgcgcacggc cgtctcgtcg gcgctctcgc cctcctcgat gtgccccttg
      901 ggaaagatcc agtcgccggt ccggcgcgag cggaccagca ccacctcggg ctccttgccg
      961 cgctgccgaa ccgcgatggc tcccgcctgt acgaccatcc gcaatgcttc tggcctcccg
     1021 gtcccgacta ctctatcctg ccaatgtgtc ggacgattca ccccgtcgtg acgcattccc
     1081 gacgacgcga atcagcctga tcctggcggc gcgcggcgag tccgagcccg tggcgcgaga
     1141 ggcgcttgcc gtgctctgcc agtcgtactg gtatccgatt tacgcctacc tccgccggct
     1201 tggccacacg caggacgcag ccgaggacct gacgcagggc ttcttcgcgc gcgtgctcga
     1261 gaagcattac ctcgacgact tcgaacggca gcgcggacgg ttccggtcgt acctgctgac
     1321 cgcgctgaag cactatgtgg cgaacgagcg ggaccgggac cacgcgcaga agcgcggcgg
     1381 cgccgtggcg acgctgtcgc tcgacaccgt gctcgcggcc gccgagacgc gctatcgcct
     1441 cgagccttcg acggagctca cgcccgagcg gatcttcgag cagcagtggg cgcttgccgt
     1501 gcttgcgcgc gtgcaggagc ggctggcggg ggaagaagcc gacggcggca ggggcgaacg
     1561 ctacgaccgc ctgaaagtgt tcctcaccgg ggaagacagc gacgtccgct accgcacgct
     1621 tgccggggaa ttgaagacga ccgaaggcgc catcaaggtg ttggtccacc ggctgcggcg
     1681 gcgattcaag gacctgctgc gcgaagagat ctcgcacacg gtggcccatg ccgacgacgt
     1741 gggcgatgag ctccggcatc tgctcacggc gctgcggggg tgaccgcgcc aggtccgcct
     1801 gaaggcggac actacgtacg tcgacttgcg cagtgtccgg cttcagccgg acccgtgacc
     1861 cgtggcgtac gtagtgtccg gcttcagctg gaccgtgatc cgccggacct tctgtgtaac
     1921 gattccgccc gaatgcttca gtacggatcg agcgcagacc acccatgacc gacgccaatc
     1981 cccctgaggc gacccgctgc agaaactgcg ggaccgagtt ctccggccgc agcacgccgc
     2041 tcgggctgtg cccggcctgc ctgatcaagc tgggcatgag cgatccgaac tggacgccgc
     2101 cggcattgcc tcccgaaaca cctctcgaca cacccgccgc cgcggcctcc gccgcgacga
     2161 tggtcgcgtc acgccgtttt ccccgcctgc cgcgcctccc ggtttcggta tggattgccg
     2221 cgggcgcgct cctggcgctg ctcttcgtcc tctcgcaatt cctgatggcg cctggccccg
     2281 gatacaacac cccgtccggc ccggtcgtcc gcttcaccat cgccctgccc gacgatgtcg
     2341 agcttgccga cggcgcgcag ttcgccgtct cgccagacgg caggcagatc gtcgccgtcg
     2401 cgcgcgaccc ccgtggacgc cagcagctct gggtccgctc gttcgcatcg cccgagtggc
     2461 gcgagcttcg tcagagcgac ggtgcgtcgc atcccttctg gtcacctgac agccgccagg
     2521 taggcttctt cgccgacagg cgactgaagc ggaccgacgt cgccagcggc ctcacctccg
     2581 tcgtctgcga cgcgccgtct ggacgcggcg gcgcctggac tgctggcggc gtgatcgtct
     2641 ttgcacccgg gtccgggccc ttgatgcgcg tgccggccgc gggcggaacg ccggagcagg
     2701 tgacgacagt cgatgagccg cgaggagcgc tcgcgcacgc ctggccgggg gtgctgcccg
     2761 acccgcagcg cttcgtgtac agcgcgtctg gcgggatcgg agagaagaac gaatcgccca
     2821 ctttcgcgcg ctcgatggac accggcgaga gccgcgtgat cgtgcaggac gggtggtcgg
     2881 ccgccttcgc gcgcggctat ctcttcttcg tgcagggcgc ctcgctcatc gcgcagccgt
     2941 tcgatgcacg tcgtctcgag gcgacgggcg acgtgcggcg ggttcccggc gcggacgagg
     3001 tgggcggggc gccgtcggag ggcgcggcgt tctcggtgtc ggacgacgtc ctcgcgttcc
     3061 ggaccgggcg cccgagtctc agtcacctcg tctggttcga tcggcggggg gaggcggtcg
     3121 ccgatgcgca ggagcccggc gacttccaac agttttccat ctcgcccgac ggaacgcgcg
     3181 tggcgatcgc gcgccgcgac gagcgggacg gatcgtcgaa catctgggcg ctcgagctgc
     3241 agcggggaac caattcgcgc gtcacgctcg ggcgcgcgcg cgattcatcg cccgtgtggt
     3301 cgccggacgc gacgcggatc gcgttcgcat ccgaccgcgg cga
//
LOCUS       DBNGSH010000476         6218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3100552, whole genome shotgun sequence.
ACCESSION   DBNGSH010000476 DBNGSH010000000
VERSION     DBNGSH010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3100552"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..674
                     /locus_tag="AC16CY_11070"
     CDS             219..674
                     /locus_tag="AC16CY_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3354376.1"
                     /translation="MVTFTVNGTTRRFDGDEDMPLLWYLRDELRLTGTRFGCGAGLCG
                     ACTVHLDGVAARSCQTPLRTLQNKRVVTIEGLSPDGRHPVQRAWVTHDVSQCGYCQTG
                     QIMQAASLLSRTPNPTDEDIDRNMAGNICRCGTYQRIRAAIKTAAKERA"
     gene            671..2935
                     /locus_tag="AC16CY_11075"
     CDS             671..2935
                     /locus_tag="AC16CY_11075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3354377.1"
                     /translation="MSTIENVSRRRFIEGLFSTGAFVLAARVLPESAWAQTPAVRTNA
                     DAAALNPSVYLGIQPDGTVFIVTHRSEMGTGIRTTLPMVAADELDADWSRVRIEQGIG
                     DARYGDQNTDGSRSIRDFYEAFRRAGASARSMLVSAAAAQWGVPASECSTRNHEVVHQ
                     ASGRRLAYGTLVPAAAKLAVPKPEELRFKPKADRRLVGQETSTYDQQDIVTGKAQFGL
                     DVFRDGMVHASIEHPPVLGGTVRSVNDKEALAVKGVQQTVTLDPFKPPHLFQPLGGVA
                     VIADNTWAATQGRRRLKVDWNDGPHASFESEAFRKQLLQTVSQPVKVARNLGNVDAEF
                     GKGGKVLEATYYTPLAAHASMEPPAAVADFRDGKVTIWAPTQNPQAVQDACAAVLGID
                     KQNVICHVTLLGGGFGRKSKPDYAAEAAVLSKKLGKPVKVVWTREDDIRFDYYHTTAA
                     VYHKASVDGRGRPTAWLHRSAFPPIASTFVPGAREPLGFELDLGLVDLPFDVPNIRAE
                     NGPADAHVRIGWFRAVSNNFHAFAAHSFADEMARAAGRDSLEYLLDLIGPGKILDLKS
                     QGVDYSNYGAPIDKYPIDTRRLRRVLEIAGERSGWGKRKPGNGWGLGIAAHRSFNTYV
                     ASVVEVQVDAKGVVKVPRIDQVVDAGVIVNPNRVRAQMEGAAVMAVGLARTGEITVAG
                     GRVRQSNFNDFRVARMNDAPYQVNVHFVDSDALPTGVGEPGLPPVLAALGNAIHAATG
                     TRVRELPLSRQKLA"
     gene            3026..4246
                     /locus_tag="AC16CY_11080"
     CDS             3026..4246
                     /locus_tag="AC16CY_11080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3354378.1"
                     /translation="MKRLMVVAAVLLLVVNAPAAAPPAIDAQGTAAISSFLRDAITRG
                     DVPGVVVMVVSPDRTLYHEAFGRMNAQGVAMQKDTIFNIASMTKAITSFGVMMLVDEG
                     KLGLDDEVSKYLPEFKSRQVISKVDLAAGTFETRPATRPITIRQLLTHTSGIGYSWSD
                     PGLALVQKKTGAADLDLPLVHEPGEKWTYGASTRVLGHVIEKISGQRIDAFLEARILG
                     PLGMRDTTYAVPSDKHSRVVTRHQKTDGKIVEIPNPNPIPATVRGDGGLYSTATDYAR
                     FVQVILNHGQLGGVRLLKEQTVRDMTRNHTGSVKVRLQPSAEPQRAKPYPLGAGEDVW
                     ALGFQLAAPAKPNPNMRRPGSLNWAGINNTYFWIDPERQIGAIVLMQMLPFYDDAAIR
                     ILQGVETRIYQHVN"
     gene            4373..6013
                     /locus_tag="AC16CY_11085"
     CDS             4373..6013
                     /locus_tag="AC16CY_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3354379.1"
                     /translation="MRKLLVACLMALVGASALLLAQERPDLDVFWKIRQEGTSRSQIM
                     RTLHVLTDVHGPRLTGSPNLKAAGEWAVEQMHAWGLKNGRLEPWNFGHPGWTNERLAA
                     YIVSPVKDSLVAEVLAWTPGTSGLVRGAAIQMTPPRQPTQEELTAYLEPLKTVVKGKM
                     VLVNPPQQVPVTFNPVPLRREDSDLLAQLNAPPAAPAGRGGGPQQAGQPGPTGQPARR
                     PVAPRQVEEQLNAFLVANGASVRLNDAGRDHGQIRAFDNRTYDVTKAPPTLVMRNEDY
                     GRIWRLLDDKRTVELEFDIVNRTYPEGRTSYNVVAEIPGTDKADEVVMLGGHLDSWHA
                     ATGSTDNAIGCAVMMEAARILSAIGVRPRRTIRVALWSGEEQGLLGSQAYIREHFGSF
                     EEQKPAFATLAAYFNIDNGTGRTRGMTVFGPNEAATVLREAVASFADLGVLGVNSTRS
                     RQAQSTDAFSFAAAGLPGINALQDPIQYQTYTWHTNLDTYERVIEDDVKKSAIVLAAA
                     VYHVAMRDQMLPRFTAEEMPRRPAPQPQQIPAETPRSN"
BASE COUNT         1122 a         2047 c         2104 g          945 t
ORIGIN      
        1 aggcacgccc gcaaagctcg tgcctcgggc aacccttatg ctggcttaga caccgctgag
       61 cgccgcatgg tctcgaggcg ccggccgacg attgtctgga tcgataatgt tgtccggttg
      121 gatccgggcc cggctcggac gcttatggct acgcggagtg gtaccatgcg gccggttggg
      181 cgttgggcgt tgggagttgg gagttggggg gttcgtaaat ggtgaccttt acagtcaatg
      241 gaacgacccg ccggttcgat ggcgacgagg acatgccgct gctgtggtat ctccgcgacg
      301 agcttcgcct gaccggcacg agattcggct gcggcgccgg cctctgcggc gcctgcacgg
      361 tgcatctgga cggcgtggcc gcgcgctcgt gtcagacgcc gctccgtacg cttcagaaca
      421 agcgcgtcgt caccatcgag ggcctgagcc ctgacggccg gcacccggtt cagcgggcgt
      481 gggtgacgca cgacgtgtcg cagtgcggct actgccagac cgggcagatc atgcaggccg
      541 cgtcgctcct gtcgcgcacg cccaacccga cagacgagga catcgatcgc aacatggccg
      601 gcaacatctg ccgctgcggc acctaccagc gcatccgcgc cgcgatcaag acggcggcga
      661 aggagcgggc atgagcacca tcgaaaacgt cagccggcgc cggttcatcg aaggcctgtt
      721 ctccacgggg gcgttcgtgc tcgccgcccg cgtgctgccc gaatccgcct gggcgcagac
      781 gcccgcggtg cgcacgaacg cggacgccgc ggcgctcaac ccgagcgtgt acctcggcat
      841 ccagcccgat ggcaccgtgt tcatcgtgac gcaccgatcc gagatgggta ccggcatccg
      901 caccacgctg ccgatggtcg ccgccgacga gctcgacgcc gactggagcc gcgtccgcat
      961 cgagcagggc atcggcgatg cccgctatgg cgaccagaat accgacggct cgcgatcgat
     1021 ccgggacttc tacgaggcgt tcagacgcgc cggcgcctcc gcgcgatcga tgctggtgag
     1081 cgcggccgcc gcccagtggg gcgtgccggc ttccgagtgc tcgacgcgca atcacgaggt
     1141 cgtgcaccag gccagcggac gacggctcgc ctatggcacg ctcgtgcccg ccgcggcgaa
     1201 gcttgcggtg ccgaaacccg aggagctccg cttcaagcca aaggcggatc ggcggctcgt
     1261 cgggcaggaa acctcaacct acgatcagca ggacatcgtc accggcaagg cgcaattcgg
     1321 gctcgacgtg ttccgcgacg ggatggtgca cgcgtcgatc gagcatccac ccgtactcgg
     1381 cgggaccgtg cgcagcgtga acgacaagga agccctggca gtgaagggcg tccagcagac
     1441 ggtcacgctc gacccgttca agccgccgca cctctttcag cctcttggcg gcgtcgccgt
     1501 gatcgccgac aacacgtggg cggcgacgca gggacgcagg aggctcaagg tcgactggaa
     1561 cgacggcccg cacgcctcct tcgaatccga ggcgttcagg aaacagctgc tgcagaccgt
     1621 cagccagcca gtcaaggtgg ctcgcaatct cggcaacgtg gatgccgagt tcggcaaggg
     1681 cggcaaggtg ctcgaggcga cctactacac gccgctcgcg gcgcacgcgt cgatggagcc
     1741 gccggccgcg gtggccgact tcagggacgg caaggtcacc atctgggcgc cgacgcagaa
     1801 tcctcaggcc gtacaggatg cgtgcgcggc cgtcctgggc atcgacaagc agaacgtcat
     1861 ctgtcacgtg acgctgctcg gcggcggctt cggacggaaa tcgaagccgg actacgcggc
     1921 tgaagcagcc gtgctttcga agaagctcgg gaagcccgtg aaggtggtgt ggacgcgcga
     1981 ggacgacatc cgcttcgact actaccacac gacggccgcc gtgtatcaca aagcatcggt
     2041 ggacggccgc ggccgtccga cggcctggct gcaccgctct gcgtttccgc cgatcgcctc
     2101 gacgttcgtc ccgggcgcgc gggagccgct cggcttcgag ctcgatctcg gcctcgtcga
     2161 tctgcccttc gatgtgccga acatccgcgc cgagaacgga cccgccgacg cgcacgtccg
     2221 tatcggctgg ttccgcgccg tgagtaacaa cttccatgcg ttcgccgcgc acagctttgc
     2281 cgacgaaatg gcgagggccg cgggccggga ttcgctggag tacctgctcg acctcatcgg
     2341 accagggaag atcctggatc tgaagtcgca gggcgttgac tactcgaact acggggctcc
     2401 gatcgacaag tacccgatcg acacccgccg gctcaggcgc gtgctcgaga tcgccggcga
     2461 gcgatccggc tggggcaagc gaaaacccgg caatggctgg ggcctcggca tcgccgcgca
     2521 ccgcagcttc aacacctacg tcgcatccgt cgtggaggtg caggtcgacg ccaagggcgt
     2581 cgtgaaggtg ccgcgcatcg atcaggtcgt ggacgccggg gtcatcgtca atcccaatcg
     2641 cgtgagggcg cagatggaag gggcggcggt gatggctgtg gggctggcgc ggacggggga
     2701 gatcaccgtc gcgggcggcc gggtccgtca gagcaacttc aacgacttcc gcgtggcgcg
     2761 gatgaacgac gcgccgtacc aggtgaacgt gcacttcgtg gacagcgacg cgctgccgac
     2821 cggcgtcggc gagccggggc ttccgccggt gctcgcggcg ctcggcaacg cgattcacgc
     2881 ggccaccggc actcgagtga gggagttgcc gttgtcgagg cagaagctgg cgtaaaacgc
     2941 gggggctgaa gcccccgctc tacggtgtcc tctcgacgcc tgcgtagagc ggaggcttca
     3001 gcctccgcgc gcgaaaggat cgacgatgaa gcgtctgatg gttgttgcgg ccgttctact
     3061 cctcgtcgtc aacgctccgg cggcagcgcc accggcgatc gacgcccagg gaacggcggc
     3121 aatatcgagc ttcctgcgcg atgccattac ccgcggcgac gtgccagggg tcgttgtgat
     3181 ggtcgtgagc ccggatcgga cgctgtacca cgaggccttc ggcaggatga acgcccaggg
     3241 cgtcgccatg cagaaggaca cgatcttcaa catcgcgtcg atgaccaagg cgatcacctc
     3301 gttcggcgtg atgatgctgg tcgacgaggg aaagctcggc ctggacgacg aggtgtcgaa
     3361 gtacctgccc gagttcaaga gccggcaggt gatcagcaag gtcgatctgg cagcgggtac
     3421 cttcgagacg cggccggcca cgcgtccgat cacgattcga cagcttctga ctcatacatc
     3481 cggcatcggc tattcgtggt cggacccggg gctcgccctg gtgcagaaga aaacgggcgc
     3541 ggccgatctc gacctgccgc tcgtccacga gccgggggag aagtggacct acggcgccag
     3601 cacgagagtc ctgggccacg tcatcgagaa gatttccggg cagcgcatcg acgcgtttct
     3661 cgaggccaga atcctcgggc cgctcggaat gcgcgacacg acctacgccg ttccttccga
     3721 caagcactca cgagtggtca cgcgacatca gaagacggac gggaagatcg tcgagatccc
     3781 caacccgaac ccgatcccgg cgacggtccg tggcgatggc gggctctatt ccacggcgac
     3841 cgactacgcg cgcttcgtgc aggtgatcct caaccacgga caactgggtg gcgtgcgtct
     3901 gctgaaggag cagaccgtcc gcgacatgac gcgaaaccac acgggcagcg tcaaggtccg
     3961 gctgcagcca tcagcggagc cacagcgcgc gaagccctat ccccttggtg cgggagaaga
     4021 cgtctgggcc ctcggcttcc agcttgcggc gccggccaaa ccgaatccga acatgcggcg
     4081 gccgggaagc ctgaactggg cgggcatcaa caacacctac ttctggatcg atcccgagcg
     4141 acagatcggc gcgatcgtgc tgatgcagat gctgccgttc tacgacgacg ccgcgattcg
     4201 gatcctgcaa ggggtggaga cgcgcattta tcagcacgtg aattgattca atggactgtc
     4261 acgaacgcta accacagaga cccggagcac accgagccca tatcgcttct ctgtgttctc
     4321 tgagcctctg tggtcaacgt ccgtgatgtg aatcgatcgt gggaggttgt gcatgcggaa
     4381 gctgctcgta gcgtgtctga tggcgctcgt cggtgcaagt gcgctgctcc ttgcccagga
     4441 gcgtccggat ctcgacgtct tctggaagat ccggcaggag ggcacgagcc gatcgcagat
     4501 catgcggacg ctgcacgtgc tgaccgacgt gcacggcccg cggctgacgg gttcgcccaa
     4561 cctcaaggcc gccggcgaat gggccgtcga gcagatgcac gcctggggcc tgaagaacgg
     4621 ccgcctggag ccctggaact tcgggcatcc gggttggacc aacgagcggc tcgcggcgta
     4681 catcgtgtcc ccggtcaagg actccctggt cgcggaggtg ctcgcgtgga cgccgggaac
     4741 cagcggcctc gtgcgtggcg cggccataca gatgacgccg ccgaggcagc cgacgcagga
     4801 agagctgacc gcgtacctcg agccgctgaa gaccgtcgtg aagggaaaga tggtgctggt
     4861 caaccctcct cagcaggtgc cggtgacctt caatcccgtt ccgcttcgac gcgaggacag
     4921 cgacctgctc gcgcagttga acgcgccgcc ggccgcgcct gccgggcgag gtggggggcc
     4981 gcagcaggca gggcagcccg ggccaactgg tcagcccgcg aggcgcccgg tggcgccacg
     5041 ccaggtcgag gaacagctga acgcgttcct ggtcgccaac ggcgcttccg tccgtctcaa
     5101 cgacgcggga cgggaccacg gacagatccg cgccttcgac aaccgaacct acgacgtcac
     5161 caaggcgccg cccacgctcg tgatgcgcaa cgaggactac ggccgcatct ggcggctgct
     5221 cgacgacaag cggaccgtcg agctcgagtt cgacatcgtg aacaggacgt acccggaagg
     5281 acgaaccagc tataacgtcg tggccgagat tcccgggacg gacaaggccg acgaagtggt
     5341 catgctcggg ggccatctcg actcctggca cgcggccacc ggatcgaccg acaacgcgat
     5401 cggctgcgcc gtgatgatgg aagccgcccg catcctgtcg gcgatcggcg tcaggccgcg
     5461 gcgcacgatc cgcgtcgcgc tctggagcgg agaggagcag gggctgctcg gctcgcaagc
     5521 gtacatccgg gagcacttcg gcagcttcga ggagcagaag ccggcgttcg ccacgctcgc
     5581 tgcctacttc aacatcgaca acgggacggg ccgcacgcgc gggatgacgg tcttcggccc
     5641 caacgaagcc gccacggtgc tgcgcgaagc ggtcgcctcg ttcgcggatc tcggcgtgct
     5701 tggcgtcaac tcgacgcgca gccgccaggc ccagagcacc gacgcgttct cgttcgccgc
     5761 cgccggactg ccggggatca acgcgttgca ggacccgatc cagtaccaga cctacacttg
     5821 gcacaccaac ctcgacacct acgagcgagt gatcgaagac gacgtgaaga agtcggcgat
     5881 cgtgctggcg gccgcggtgt accacgtggc gatgagggac cagatgctgc cgcgcttcac
     5941 ggccgaggag atgccgaggc gtcccgcccc gcagccgcag cagatacccg cggagacgcc
     6001 acggagcaat tgatcccggt gacagtcgca gtgtccgccg tcagccggac cctgaaccta
     6061 tgacgctcga cacgccgggc gaggagctca gacgccgctt cggcgacatc gacatctacc
     6121 tgttcgacca gatcctgcgc ggacgcttcg acgcgaggcg gcgtgtgctc gacgccggct
     6181 gcggcgacgg acgtaacctg atctacttcc tgcagcac
//
LOCUS       DBNGSH010000477         2458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1063155, whole genome shotgun sequence.
ACCESSION   DBNGSH010000477 DBNGSH010000000
VERSION     DBNGSH010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1063155"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2091
                     /locus_tag="AC16CY_11090"
     CDS             <1..2091
                     /locus_tag="AC16CY_11090"
                     /inference="COORDINATES: protein motif:HMM:NF036531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR3354380.1"
                     /translation="AGDRSSRFGSGDTEVRTNKPLTLRPTFPRFLAVGDSARFGAAVG
                     SQLPSDGTAVVSMRSLDPRVLEIVGGAEQRVKIPSGGSLEVRFSGSAKAIGRARIQMR
                     ARIGSESDAFEDVIPVEVLASPETVSAYGEAAAPNTVARETIRLPSGVIRGFGGLNIE
                     LSSTAMVGLGEGARYLVEYPYGCAEQKASASLALVLAADLGDAFTLPGMDPARMRPVA
                     QKTLKELETFQCGNGGFAYWPGACWTTSPYLTAYILHVFKTASDLKYDVDRGVRDRGY
                     SYLERELAASPPDVNEGWWPAYTAWQAFAVKVLAEGGRSQDSHLTRLYGYRDRMPVFA
                     LAYLHDAMLARGEAAGDRITDLRRRMANAILPEGGSAHVEELADPYLLWFWNSNVRST
                     AIVLNSLVNGSVNDAPMRQIVRWMMAARKDGRWGNTQENAHAMQALVAYYRKYESAVP
                     DFRAVVSLGARELARDEFRGRSTESSATTVPLARLISAGPAGTDVPLTFRRDGSGTLF
                     YAARLQYAVDNLYQEGLDSGFHIERRYEPYVDPSTALGAGPSTALGARPPSTTFAAGD
                     MVRVTLTFRLTKERRFVAVTDPLPAGFEAVESWFATTARDVAEQQDRQRNDGGDASDW
                     RNWWRHSGFDRVERHDDRVRLFATRLSEGLHEFSYIVRATTAGSFRTAPTRAEEMYEP
                     EVFGRTSTTVIQVK"
     gene            2135..>2458
                     /locus_tag="AC16CY_11095"
     CDS             2135..>2458
                     /locus_tag="AC16CY_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3354381.1"
                     /translation="MKGTGGRGKGIVVAAVVILALVWLRMGPLPAGLLDEAPNPSTVV
                     VDRNGVPLHEALSSDDTRVVQLTAAALPPLLVAATLAAEDRRFWRHPGVDPIAILRAL
                     KTNVAE"
BASE COUNT          388 a          809 c          885 g          376 t
ORIGIN      
        1 gcaggcgatc gcagctcgcg cttcgggtcc ggcgacaccg aagtccgtac gaacaagccg
       61 ctgacgctcc ggcccacgtt cccgcggttc ctcgcggtcg gcgacagcgc ccgttttgga
      121 gcggcggtgg gaagtcagct tccctcggat gggacggccg tcgtctcgat gaggtcgctc
      181 gatccgcgcg tgctcgagat cgtcgggggg gccgagcagc gcgtgaagat tccctcgggt
      241 ggatcgcttg aagtgcgctt ctccggttcg gcgaaggcga tcggccgcgc gcgaatccag
      301 atgcgcgccc ggatcggcag cgagagcgac gcgttcgagg atgtgattcc ggtcgaggtg
      361 ctcgcctctc ccgagacggt ctcggcgtac ggcgaggcgg ccgcaccgaa caccgtggcc
      421 agggagacca tccgccttcc atcgggcgtg atccgcgggt tcggcggcct gaacatcgag
      481 ctgtcgtcca ccgcgatggt gggtctcggc gagggcgcgc ggtacctcgt cgagtatccg
      541 tacggctgcg ccgagcagaa ggcgtccgcc tcgctcgccc tggtgctggc ggcggatctg
      601 ggcgacgcgt tcacgctgcc gggaatggat ccggccagga tgcggccggt cgcgcagaaa
      661 acgctgaagg agctcgagac gttccagtgc ggcaacggcg gcttcgcgta ctggccgggc
      721 gcctgctgga cgacgtcccc gtacctcacg gcgtacatcc ttcacgtgtt caagaccgcg
      781 tcggacctga aatacgacgt cgatcgcggc gtgcgcgatc gcgggtacag ctacctggaa
      841 cgcgagctcg ccgcgagccc gccggacgtc aacgagggat ggtggccggc ctacaccgcg
      901 tggcaggcgt ttgcggtgaa ggtgctcgcc gaaggcgggc gcagccagga ctcgcacctc
      961 acgaggctct acggctaccg ggatcgcatg cccgtcttcg cgctggccta cctgcacgac
     1021 gccatgcttg cgagaggcga ggcggccggc gatcgcatca ccgacctgcg gcgccgcatg
     1081 gccaacgcga tcctgccgga aggcggcagc gcccacgtcg aggagctggc cgatccctac
     1141 ctgctctggt tctggaactc gaacgtgcgc tcgacggcga tcgtgttgaa ttcgctcgtc
     1201 aacggcagcg tgaacgacgc gccgatgcgc cagatcgtgc gctggatgat ggccgcacgg
     1261 aaggacggcc gctggggaaa cacgcaggag aacgcgcacg ccatgcaggc gctcgtcgcg
     1321 tattaccgca agtacgaatc ggccgtgccc gacttccgcg cggtcgtgtc gctcggcgcg
     1381 cgcgagctgg cgcgcgacga gttccgcgga cgctcgaccg aatcgtccgc gacaaccgtt
     1441 ccactggcgc gcctgatatc agcgggtccg gccggcacgg acgttccgct gaccttccgg
     1501 cgcgacggga gcggcacgct cttctacgcc gcccggctgc aatacgccgt ggacaacctc
     1561 taccaggagg ggctcgattc gggattccac atcgagcgcc ggtacgagcc gtatgtcgac
     1621 ccctcgactg cgctcggggc aggcccctcg accgcgctcg gggcaaggcc gccgtccacg
     1681 accttcgccg cgggcgacat ggtccgcgtg acgctgacgt tccgcctgac aaaggagcgg
     1741 cgctttgtcg ccgtcaccga tccgcttccg gccgggttcg aggcggtcga atcgtggttc
     1801 gcgacgaccg cgcgcgacgt ggcggagcag caggatcgcc agcgcaacga cggcggcgac
     1861 gccagcgact ggcgcaactg gtggcgtcac agcggattcg atcgcgtgga gcgtcacgac
     1921 gatcgcgtcc ggctgtttgc caccaggctc agtgaaggcc tccacgagtt cagctacatc
     1981 gtccgcgcca cgaccgcggg atcgttccgg acggcgccga cgcgagccga ggagatgtac
     2041 gagccggagg tcttcgggag aacttcgacg acggtgatac aggtcaaatg aaggggacag
     2101 ggggcagggg gcagggggca gggggaaggg gacagtgaag gggacagggg gcagggggaa
     2161 ggggatagtg gtggcggccg tcgtcatcct tgccctggtg tggctgcgca tgggtccgct
     2221 gccggcaggc ctgctcgacg aagcgcccaa cccgtcaacc gtcgtcgtcg atcgaaatgg
     2281 cgtgccgctt cacgaggcgc tctcgagcga cgacacgcgc gtcgtgcagc tcacggccgc
     2341 ggcgcttcca cccctgctcg tcgcggcgac attggctgcc gaggatcggc gcttctggcg
     2401 gcaccccggc gtcgatccga ttgcgatcct gcgtgcgctc aagacgaacg tggccgag
//
LOCUS       DBNGSH010000478         2711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3609815, whole genome shotgun sequence.
ACCESSION   DBNGSH010000478 DBNGSH010000000
VERSION     DBNGSH010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3609815"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="AC16CY_11100"
     CDS             <1..181
                     /locus_tag="AC16CY_11100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3354382.1"
                     /translation="LLATAGLAAVTADLVSIGVAATTWVSLPVQARLEEEFLLARYPK
                     EYPAYAGTTGRFWPK"
     gene            296..838
                     /gene="pyrR"
                     /locus_tag="AC16CY_11105"
     CDS             296..838
                     /gene="pyrR"
                     /locus_tag="AC16CY_11105"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR3354383.1"
                     /translation="MPVVMDADRIGRALTRIAHEIVERNKGTDNLALVGVRTRGVPIA
                     RRIARTLREITGQEVPTGALDITMYRDDLMRHAVGPQPVVRRTEIQFSIDNFKILLVD
                     DVLYTGRTTRAALDALIDYGRPKAIQLIVLVDRGHRELPIKADYVGKNLPTAPEQSVQ
                     VRLQEIDGEDEVVLEGGGAA"
     gene            835..1815
                     /locus_tag="AC16CY_11110"
     CDS             835..1815
                     /locus_tag="AC16CY_11110"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR3354384.1"
                     /translation="MSAISHETDQKPAVTALRHKDLLGIAELEPDEIELILDTAEAMK
                     EVGTRPIKKVPALRGKTVVNLFFEPSTRTRTSFEIAEKRLSADTLNIAIGTSSVVKGE
                     TLADTALNLEAMSPDMIVLRHASSGACHLLSRICRSAIINAGDGMHEHPTQALLDAFT
                     IRERRRQLTGLKVAIVGDLLHSRVLRSNIHLLTKMGADVWVCGPPTLFPTDIRRFGVT
                     ATSVVDDAVRDADVIMLLRIQLERMEGAFFPSLREYFRVFGMTESRVRLAKPDVMIMH
                     PGPMNRGVDIASEVADGPYSVILDQVANGVAVRMAVLYLLAGGAEHEEAA"
     gene            1799..>2711
                     /locus_tag="AC16CY_11115"
     CDS             1799..>2711
                     /locus_tag="AC16CY_11115"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3354385.1"
                     /translation="MKRLLKGGRVVDPANGVDAVCDVLIEDGRIARVGVGLPVNGADV
                     VEIPAGLVVCPGFIDMHVHLREPGQEHKETVATGVASAVAGGFTAVACMPNTVPVNDN
                     ANVTALILAKAAEANLAHVYPIGAVSKASQGELLADIAELKQAGCVAITDDGRPVSTA
                     LLLRRALEYAGMFGMPVIEHCEDPTLKGDGVAHEGYHAATLGLRGIPGAAEALGVERG
                     VLLSELTGSAFHVAHMSARASIRAVRKGKDAGVRVTCEVAPHHFTLTDQALTAPIPYD
                     TNVKMNPPLREAADRDAMLAGIADGTVD"
BASE COUNT          468 a          912 c          926 g          405 t
ORIGIN      
        1 gctgctcgcg acggccggtc tcgcggccgt gactgccgac ctcgtctcga ttggcgtcgc
       61 cgcgaccacc tgggtgagcc tgcccgtgca agcccgcctg gaagaggaat tcctgctggc
      121 gcgctatccg aaggagtacc cagcctacgc ggggaccaca ggacgtttct ggccgaagta
      181 aacccctgct acaatagatt cgcttcttta acgcggccca gagagtccgc aaagaggtgc
      241 acatgcccgc acgcgtcttc ctcgatacag gctccacacc caccgcgagg cccgcatgcc
      301 agtcgtaatg gatgcggatc gcattggccg tgccctgacg cgcatcgcgc acgaaatcgt
      361 cgagcgtaac aagggaaccg acaatctcgc gctcgtcggc gtccggacgc gcggtgtgcc
      421 gattgcccgc cgcattgccc gcacgctccg ggagatcacc gggcaggagg tccccaccgg
      481 cgcgctcgac atcaccatgt accgcgatga tctgatgcgc cacgccgtcg gaccccaacc
      541 cgtggtccgc cggacggaga tccagttctc catcgacaac ttcaagattc ttctcgtcga
      601 cgacgtgctc tacacgggcc ggacgacgag ggcggcgctc gacgcgctca tcgactacgg
      661 ccggccgaag gccatacagc tcatcgtgct ggtggatcgc gggcatcggg agctgccgat
      721 caaggcggac tacgtgggga agaacctgcc gaccgctccc gaacagagcg tgcaggtgcg
      781 gctgcaggag atcgacggcg aggacgaggt cgttctggaa gggggaggtg cggcatgagc
      841 gcgatcagcc acgagaccga tcagaaaccg gcggtcacgg cccttcgcca caaggacctc
      901 ctcggcatcg ccgagctgga gcccgacgag atcgagctga ttctcgacac ggccgaagcg
      961 atgaaggaag tgggcacccg gcccatcaag aaagtccccg cgctccgcgg caagaccgtg
     1021 gtcaacctgt tcttcgagcc gagcacgcgg accaggacct cctttgagat cgccgagaag
     1081 cgcctcagcg ccgacacgct gaatatcgcg atcggcacct cgagcgtcgt caagggggag
     1141 acgctggcgg ataccgcctt gaacctcgag gcgatgtcgc cggacatgat cgtcttgcgc
     1201 cacgcgtcct ccggcgcgtg ccacctgctg tcgcggatct gccggtcggc gatcatcaat
     1261 gccggcgacg gcatgcacga gcatcccacg caggccctgc tcgacgcctt caccatccgc
     1321 gaacgtcgcc ggcagctgac cggactcaag gtcgcgattg tgggcgacct gctccacagc
     1381 cgcgtgctgc gctcgaacat tcacctgctg accaagatgg gcgccgacgt ctgggtgtgc
     1441 ggcccgccga cgctgttccc gaccgacatc cgccggttcg gcgtcaccgc caccagcgtg
     1501 gtggacgacg cggtgcggga cgcggacgtg atcatgctgc tcaggatcca gctggaacgc
     1561 atggaaggcg ccttcttccc gtcgctgcgc gagtatttcc gcgtgttcgg catgaccgaa
     1621 tcgcgcgtgc ggctggccaa gccggacgtc atgatcatgc accccggtcc gatgaatcgc
     1681 ggcgtcgata tcgcgtcgga agtcgccgac ggcccgtact cggtcatcct ggaccaggtg
     1741 gccaacggcg tggccgtgcg gatggcggtg ctgtacctcc tggcgggcgg agcagagcat
     1801 gaagaggctg cttaagggcg gacgggtcgt cgatcccgcc aacggggtgg acgccgtgtg
     1861 cgacgtcctg atcgaagacg ggcgcatcgc gcgcgtcggc gtcggcctgc cggtcaacgg
     1921 ggcggacgtc gtggagattc cggcggggct cgtggtgtgt ccaggcttca tcgacatgca
     1981 cgtgcacctg cgggagccgg gacaggagca caaggaaacc gtggccaccg gcgtcgcgtc
     2041 cgcggtggcc ggcggattca ccgccgttgc gtgcatgccg aatacagtgc ccgtcaacga
     2101 caacgccaac gtgacggcgc tcatcctggc caaggcggcc gaggcgaacc tcgcgcacgt
     2161 gtacccgatc ggcgccgtgt cgaaagcatc gcagggggag ctgctggccg acatcgcgga
     2221 gctgaagcag gccgggtgcg tcgcgatcac cgatgacggc cggccggtct cgacggccct
     2281 gctgctgcgg cgcgcgctcg agtacgcggg catgttcggc atgcccgtga tcgagcactg
     2341 cgaggacccg acgctcaagg gcgacggcgt cgcgcacgag ggctatcacg ccgcgacgct
     2401 cggcctgcgc ggcatcccgg gcgcggccga ggcgctcggc gtggagcgcg gcgtcctgct
     2461 gtcggagctg accgggtcgg ccttccacgt ggctcacatg agcgcgcggg cgtcgatcag
     2521 ggcggtgcgc aagggcaagg acgccggcgt gcgtgtgacc tgcgaggtgg cgccgcacca
     2581 cttcacgctg acggaccagg cgctcaccgc gccaatcccc tacgacacga acgtgaagat
     2641 gaatccgccg ctgcgcgagg cggccgaccg cgacgccatg ctcgcgggaa tcgccgacgg
     2701 gacggtcgac g
//
LOCUS       DBNGSH010000479         4890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1572849, whole genome shotgun sequence.
ACCESSION   DBNGSH010000479 DBNGSH010000000
VERSION     DBNGSH010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1572849"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..3131
                     /locus_tag="AC16CY_11120"
     CDS             258..3131
                     /locus_tag="AC16CY_11120"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HIR3354386.1"
                     /translation="MERGPAREAAAGQQVTQGGPLDPSTRVRKVESKPTLVPGLEFPR
                     YFTLPGVDPFDEIEWEIRSAVIGNEKGKVVFEQRDVEIPQFWSQQATNIVVSKYFRGQ
                     IGTPERERSVKQLVGRVVDTITGWARVQKYFASEDALVSFSSELKHLLVYQKAAFNSP
                     VWFNCGFEKAPQCSACFINSVQDTMDSILTLAKTEGMLFKYGSGTGSNLSAIRSSKEL
                     LAGGGTASGPVSFMKGYDAFAGVIKSGGKTRRAAKMVILNADHPDVMDFINCKVEEEK
                     KAWALIDAGYDGSFTGTAYTSIFFQNSNNSVRVTDDFMRAVLDDGNWHTKAVRDGSVM
                     ETYKARDLMHGIAEGTWVCGDPGMQFDTTVNEWHTCPNTARINASNPCSEYMFLDDSA
                     CNLASINLMKFVKEDGEFDVPAFKAACRTLITAQEIIVPNSSYPTKSIEKNSHEYRPL
                     GLGYANLGALLMSRGLPYDSDEGRAYAAAITAVMTGEAYAQSAKVARDHGGPFAGFDA
                     NREPMLRVMRKHRDAVSEIAPKYVPADLYAAARQAWDEAVELGEDFGYRNAQATVLAP
                     TGTIGFMMDCDTTGVEPDIALVKYKKLVGGGLMKIVNTTVPMALAKLGYTKPEIEAIV
                     HHIDEQETIEGAPFLRDEHLPVFDCAFKPANGERSIQYMGHIRMMGATQPFISGAISK
                     TVNVPREASVEDIERAYLESWRLGAKAISIYRDGSKRTQPLNTSKAQVTDTRNVETVR
                     EVVKEVVKIVERPQRRKLPDERNSITHKFDVSGHEGYITVGLFEDGTPGEIFLVMAKE
                     GSTISGFADAFAQAVSYCLQYGVPLQTLVDKFSHSRFEPSGMTKNPEVRFAKSIVDYI
                     FRWMATKFLSPEAQFRAGVNMREEPDQVSGSMPGSTSSTGAMFSAIPEVKVEADRRAK
                     KTSEFGALRNQEDAPPCSTCGSIMIRSGSCYKCGNCGTTSGCA"
     gene            3286..4119
                     /locus_tag="AC16CY_11125"
     CDS             3286..4119
                     /locus_tag="AC16CY_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354387.1"
                     /translation="MSALLASIPVSAQWFKYPTAGVPRKADGSVNMSAPAPRMADGKP
                     NFSGIWMTAEPNRRAEGLSSPGNPVSPREAKPIDEKPGDPTAIGASRQMANIGVDLPG
                     GLPYQPWLVPIVKERTANDAIDDPHIRCLPDNFLRAYGLPHLLKFVHTPGLLVVLNEM
                     NATYRQVFTDARPLPDDPNPSWQGYSSAKWSGDTLVIDTIGVRDDTWIDWNGSVLTQS
                     AKVREEIRRPDFGHLEVRATVDDPKAYTKPWTVTLRQRIVVDTELVDEICLEHQKFLV
                     R"
     gene            4121..4852
                     /locus_tag="AC16CY_11130"
     CDS             4121..4852
                     /locus_tag="AC16CY_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3354388.1"
                     /translation="MGFIGFLYPWGILLQVLAVIHFFRRRPETFWIYVIFFLGPPGAL
                     IYIVMEVVPDLGLLRQSFDSFGRRKRIRLLEALIVQNPSAGNYEELGDLYLEEKKFAK
                     ARECYDKAISPRTAHLDPIYRRAIAEIQLGDFAAAATDLEQVTSRDAKYDSHRAVGLL
                     AHAFANTGRAEEAEALFRQATERSTLTETQVNYATFLASQNRPQEARQWAEAVLERKP
                     TMPRYLQRRERPWFRKAKAVLKRVK"
BASE COUNT          951 a         1558 c         1598 g          783 t
ORIGIN      
        1 ggtagaatgg aaccaacata ttgcgcttgc cctaaaagaa aaacgcaata tgttgtggtt
       61 agcgtcttga caaagctgta aaccgggcgc actatgaccg ggcgctcaac gaggtgtaga
      121 caaggcagga agctcccagc ctccggccga tcgggttagg cccacaggac gtctcgcgcg
      181 ccaaccacaa acagaattcc ggacgccacc gaaggggtag gtgcgtcctt gagggaagcg
      241 tcatgtcgga ggagagcatg gaaagaggcc ctgcccgcga agctgccgcc ggccagcagg
      301 tgacacaggg tggccccctc gacccctcga cgcgggttcg caaggtcgag tcgaagccga
      361 cgctcgtgcc cggcctcgag tttccacgct atttcaccct gccgggcgta gacccgttcg
      421 acgagatcga atgggaaatc cgctccgcgg tgatcggcaa tgaaaagggc aaggtcgtct
      481 tcgagcagcg cgacgtcgag attccccagt tctggtccca gcaggccacc aacatcgtcg
      541 tctcgaagta tttccgcggg cagatcggga cgccggagcg ggaacggagc gtcaagcagc
      601 tggttggccg cgtggtggac acgatcaccg gctgggcgcg ggtgcagaag tacttcgcca
      661 gcgaggacgc gctcgtttcc ttcagcagcg agctgaagca cctgctcgtc tatcagaagg
      721 cggcgttcaa cagcccggtc tggttcaact gcggcttcga gaaggcgccc cagtgctcgg
      781 cgtgcttcat caactccgtg caggacacga tggactcgat cctgacgctg gccaagacgg
      841 aggggatgct cttcaagtac ggctccggca ccggcagcaa cctctcggcg atccgttcgt
      901 cgaaggagct gctcgccggc ggcggcacgg cgtccggccc ggtctcgttc atgaagggct
      961 acgacgcgtt tgccggcgtg atcaagtcgg gcggcaagac gcggcgcgcc gcgaagatgg
     1021 tgatcctcaa cgccgaccat cccgacgtga tggatttcat caactgcaag gtcgaggagg
     1081 agaagaaggc ctgggcgctc atcgacgccg gctacgacgg atccttcacc ggcacggcct
     1141 acacctccat cttcttccag aactccaaca acagcgtgcg cgtcaccgac gacttcatgc
     1201 gcgcggtgct cgacgacggg aactggcaca cgaaggcggt gcgggacgga tcagtgatgg
     1261 agacctacaa ggcgcgcgac ctcatgcacg ggatcgcgga gggcacgtgg gtgtgcggcg
     1321 atcccggcat gcagttcgac accaccgtca acgagtggca cacctgcccg aacacggcgc
     1381 gcatcaatgc gagcaacccg tgctcggagt acatgttcct ggacgactcg gcgtgcaacc
     1441 tcgcctcgat caacctgatg aagttcgtca aggaggacgg cgagttcgac gtccccgcgt
     1501 tcaaggcggc gtgccgcacc ctcatcaccg cgcaggagat catcgttccg aactcgagct
     1561 acccgaccaa gtcgatcgag aagaactcgc acgagtaccg tccgctcggg ctcggctacg
     1621 cgaacctcgg cgccctgctc atgtcgcgcg ggctgccgta cgacagcgac gaagggcgcg
     1681 cgtacgcggc cgccatcacc gcggtgatga ccggcgaggc gtacgcccag tcggcgaaag
     1741 tcgcacgcga ccacggcggc cccttcgccg gcttcgacgc caaccgcgag ccgatgctgc
     1801 gcgtcatgcg gaagcaccgg gacgccgtgt cggagatcgc cccgaagtac gtgccggccg
     1861 acctgtacgc ggcggccagg caggcatggg atgaagccgt ggagctcggc gaggacttcg
     1921 gctaccggaa cgcgcaggcg acggtgctcg cgccgaccgg gacgatcggc ttcatgatgg
     1981 actgcgacac cacgggcgtc gagcccgaca tcgcgctcgt caaatacaag aagctcgtcg
     2041 gcggcgggtt gatgaagatc gtcaacacta ccgtgccgat ggccctcgcc aagctcggct
     2101 acaccaagcc ggagatcgag gcgatcgtgc accacatcga cgagcaggag acgatcgaag
     2161 gggcgccgtt cctcagggac gagcacctgc cggtgttcga ctgcgcgttc aagccggcca
     2221 acggcgagcg gtcgatccag tacatgggtc acatccggat gatgggcgcc acgcagccgt
     2281 tcatctcggg cgccatcagc aagaccgtca acgtgccccg cgaagcgagc gtcgaggaca
     2341 tcgagagggc gtacctggag tcgtggcgtc tcggcgcgaa ggcgatctcc atctaccgcg
     2401 acggcagcaa gcggacgcag ccgctcaaca cgtcgaaggc gcaggtcacc gacacgcgca
     2461 acgtcgagac cgtgcgggag gtcgtgaagg aggtcgtgaa gatcgtcgag cggccgcagc
     2521 ggcgcaagct ccccgacgag cgcaactcca tcacgcacaa gttcgacgtc tccggccacg
     2581 agggctacat cacggtcggc ctcttcgagg acgggacgcc cggcgagatc ttcctggtga
     2641 tggcgaagga ggggtccacg atctcgggct tcgccgacgc gttcgcgcag gcggtctcct
     2701 actgcctgca gtacggcgtc ccgctccaga cgctggtgga caagttcagc cactcgcgtt
     2761 tcgagccgtc ggggatgacg aagaaccccg aggtgcgctt cgccaagtcg atcgtcgact
     2821 acatcttccg gtggatggcc acgaagtttc tgtcgcctga agcccagttc cgcgcgggtg
     2881 tgaacatgcg cgaggagccg gaccaggtgt caggcagtat gcctggttca accagttcaa
     2941 cgggtgcgat gttcagcgcg atcccggagg tcaaggtcga agccgacagg cgagcaaaga
     3001 agacgagcga gttcggcgcg ctgcggaacc aagaggacgc gccgccgtgc agcacgtgcg
     3061 ggtcgatcat gatccgcagc ggcagctgct acaagtgcgg caactgcggc accacgagcg
     3121 gctgcgcata gaaggcctca gggcccgggc ctcaggcctc agggctgggc agggaatcag
     3181 tgggcgcaga gaaacggtag gggcgggtct tcagacccgc ccgacaacga ccgttgaagt
     3241 atccttcccg gcatgttctt tcagcgttca gccgttctcg tcgttgtgag cgctctcctg
     3301 gcttcgattc ctgtctccgc tcaatggttc aagtacccga cggcgggagt tccacggaag
     3361 gcggatggca gcgtgaacat gtcggctccc gcgccccgga tggccgacgg caagccgaac
     3421 ttctccggaa tctggatgac ggccgaaccc aatcggcgag cggaggggct gtcgagccca
     3481 ggcaatccgg tcagtccacg ggaggcgaag cccatcgacg agaagcccgg tgatcccact
     3541 gcgatcggcg cgtcccgcca gatggcgaac atcggcgtcg atcttccggg aggcttaccg
     3601 tatcagccct ggctcgtccc catcgtcaaa gagcgcacgg ccaacgacgc gatagacgac
     3661 ccgcacatcc gatgcctgcc tgacaacttc ctgcgcgcgt acgggctgcc gcacctgctg
     3721 aagttcgtcc acacgccggg gctcctggtg gtgctcaatg aaatgaatgc cacgtaccgc
     3781 caggtgttca ccgacgcccg ccccctgccc gacgatccga acccgtcgtg gcaggggtat
     3841 tcgtcggcga aatggtccgg cgataccctt gtgatcgaca cgatcggggt gcgcgacgac
     3901 acgtggatcg actggaatgg aagcgtgctg acgcagtcgg ccaaggtgcg ggaggaaatc
     3961 cggcgtcccg acttcggaca cctggaggtc cgggccacgg tagacgatcc caaggcgtac
     4021 acgaagccgt ggacggtcac gttgcggcag cgcatcgtcg tcgacacgga gttggtggac
     4081 gaaatctgcc tggaacacca gaagttcctc gtgcgctgac atgggcttca tcggcttcct
     4141 gtatccgtgg ggcatcctgc tgcaggtgct ggcggtcatc cacttcttcc gccgccgccc
     4201 ggagacgttc tggatctacg tcatcttctt tctcggtcca cccggcgcgc tgatctacat
     4261 cgtcatggag gtcgtcccgg atctgggcct gctccggcag tcctttgatt cattcggacg
     4321 gcgcaagagg atccgccttc tcgaggcgct catcgtgcag aacccgtcgg ccgggaatta
     4381 cgaggagctc ggcgacctgt acctcgaaga gaagaagttc gcgaaggcac gcgagtgcta
     4441 cgacaaggcc atctcgcccc gcacggccca cctcgatcca atttatcgcc gcgcgatcgc
     4501 cgagatccag cttggcgact ttgctgccgc cgccacggat ctcgagcagg tcacgtcgcg
     4561 cgacgccaag tacgattccc accgcgccgt ggggctgctg gcccatgcct tcgcgaacac
     4621 ggggcgtgcc gaagaggccg aggcgctctt ccgtcaggcc acggagcggt ccacgctgac
     4681 cgagacccaa gtgaactacg cgacgtttct cgcgtcgcag aaccggccgc aggaggcgcg
     4741 ccaatgggcc gaggccgtcc tggagcgcaa gccgaccatg ccaaggtatc tgcagcggcg
     4801 ggagcggccg tggtttcgga aggccaaggc cgtcctgaag cgcgtcaagt aaatggggtc
     4861 gggagtcatt tcttgaggac actctggtgc
//
LOCUS       DBNGSH010000480         1844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2252284, whole genome shotgun sequence.
ACCESSION   DBNGSH010000480 DBNGSH010000000
VERSION     DBNGSH010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2252284"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..622)
                     /locus_tag="AC16CY_11135"
     CDS             complement(101..622)
                     /locus_tag="AC16CY_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3354389.1"
                     /translation="MFTHHHDERPYSFCPLCGGELDLRSIKATEPDRLVCRRCGFIFY
                     QDPKVAVGTIIRDDHGQIVLVRRAIEPGYGRWVFPGGFVDRGETVLAAAVREAREEAG
                     LDIQIERLLNVYSYPGRAPVIIVYVAKMTGGTLTCDDESLEGGFFARGDIPWNELAFQ
                     STHEALEEFFRAG"
     gene            643..1170
                     /gene="def"
                     /locus_tag="AC16CY_11140"
     CDS             643..1170
                     /gene="def"
                     /locus_tag="AC16CY_11140"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3354390.1"
                     /translation="MSILKVARMGHPVLRERAREVSKSDLLNPLVQKLVDDMIDTMRE
                     YHGVGLAAPQVHEGLRLFVALLDEEPGASSDVVAVVNPEIVPASPAQEEGWEGCLSIP
                     DIRGRVPRFTDVTVSALDREGKPLELKLKGFPARVAQHETDHLDGILFFDRMRSMESL
                     TFLDEYSRFHEKDED"
     gene            1170..>1844
                     /locus_tag="AC16CY_11145"
     CDS             1170..>1844
                     /locus_tag="AC16CY_11145"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR3354391.1"
                     /translation="MPQTTLALWTGSPVVTLRTAPQAPYDGAIAAARTCYSPRVIGAG
                     EVTESQRDSIGALTFDAGHHTVYQHAHFEFGLENISRQFVWTFLHSYPFYNSEQSSQR
                     YVKLKEPRAYVPPLSGEALGVYEQAVVRAWDRYAELAALLEGDALAILKELRYVRPNT
                     SPERLKLIEKDAGKRAIETARYVIPIGAFTSMVHTVSGIVLHRLHRMMNTGDAPHETC
                     HVISEMV"
BASE COUNT          326 a          595 c          605 g          318 t
ORIGIN      
        1 tggcttcgtg gaagttccgg gcgaaacgcg ctcgttcgag tcccccgttg agccacggat
       61 acgaaccccg agtatgtaat ttggtaatgg gcgaagaggg tcagccagcc cggaagaatt
      121 cctcgagtgc ctcgtgcgtg ctctgaaacg cgagctcgtt ccaggggatg tcgccccggg
      181 cgaaaaaccc gccctcgagc gattcgtcgt cgcacgtcag cgtgccgccg gtcattttcg
      241 cgacgtagac gatgatgacc ggcgcgcggc cgggatagga gtagacgttg aggagccgct
      301 cgatctgaat gtcgagcccg gcttcctccc gcgcctccct gactgccgcc gccagcaccg
      361 tctcgccccg atcgacgaaa ccgcctggga acacccagcg gccgtacccc ggttcgatcg
      421 ccctgcgcac gaggacgatt tggccgtggt cgtcgcggat gatcgttccg acggcgactt
      481 ttggatcctg gtagaagatg aagccacagc ggcggcagac cagtcgatcg ggttcggtcg
      541 ccttgatcga gcgcagatcc agctctccgc cgcaaagagg gcagaagctg tacgggcgct
      601 cgtcgtgatg atgcgtgaac aagcctttat aatctaccct ctatgtcgat cctgaaagtc
      661 gcgcgcatgg gtcatcccgt tctgcgggag cgcgcgcgag aggtgtcgaa gtccgatctg
      721 ctgaacccgc tggtgcagaa gctcgtggac gacatgatcg acaccatgcg cgagtatcac
      781 ggcgtcggcc tcgccgcgcc ccaggtccac gagggtctgc gtctgttcgt ggcgctgctc
      841 gacgaggagc cgggggcgtc cagcgatgtg gttgcggtgg tgaaccccga gatcgttccc
      901 gcgagccccg cacaggagga aggctgggag ggctgtctca gcatccccga catccgcggc
      961 cgcgtgccca ggttcacgga cgtgaccgtg tcggcgctgg atcgcgaggg caagccgctc
     1021 gagctgaagc tgaaagggtt tcccgcgcgc gtcgcacagc acgagacgga ccatctcgac
     1081 ggcatcctgt tcttcgatcg gatgcgatcg atggagtcgc tcacgtttct cgacgagtac
     1141 tcgcgttttc acgagaaaga cgaagactag tgccacagac cacgctggcg ctctggaccg
     1201 gctcgcccgt cgtgaccctc cggaccgcgc cgcaggctcc ctacgacggc gcgatcgcgg
     1261 cggcgcggac ctgctactcg ccgcgcgtga tcggcgcggg cgaggtcacc gagtcgcagc
     1321 gcgattcaat cggggccctc accttcgatg ccggccatca cacggtgtac cagcacgcgc
     1381 acttcgagtt cgggctggag aacatctccc gccagttcgt gtggacgttc ctgcactcgt
     1441 accccttcta caactcagag cagtccagcc agcgctacgt gaagctgaag gagccgcgcg
     1501 cgtacgtgcc gcctctcagc ggtgaggcgc tgggcgtcta cgaacaggca gtggtccggg
     1561 cgtgggatcg ctacgcggag ctcgctgcac tgctcgaagg cgatgccctg gcgatcctga
     1621 aggagctgcg ctacgtccgg ccgaacacca gccccgagcg gctgaagctg atcgagaaag
     1681 acgccgggaa gcgcgcgatc gagacggcgc gctacgtcat cccgatcggc gcgttcacct
     1741 ccatggtgca cacggtgtcg ggcatcgtgc tgcatcgcct ccaccggatg atgaacacgg
     1801 gcgacgcgcc gcacgagacg tgccacgtga tctcggagat ggtc
//
LOCUS       DBNGSH010000481         4074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2421922, whole genome shotgun sequence.
ACCESSION   DBNGSH010000481 DBNGSH010000000
VERSION     DBNGSH010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2421922"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="AC16CY_11150"
     CDS             <1..758
                     /locus_tag="AC16CY_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HIR3354392.1"
                     /translation="VARQVDWVYDFALPPLVLHTLYTRDATPLKRWLEMRPRNAVTVL
                     DTHDGIGLQDAGADDRRPGVPGLLPAEAIDALGETIHQRSGGESRQASGTAASNVDEH
                     HINCAFYDALGRRDDEYLVARAIQCFVPGIPQVYYVGLLAGGNDMELLRRTGVGRDIN
                     RHYYTGAELQRDLSRPVVRALLALLRFRNSHAAFEGACDLAPSAGNSVVLEWRNGAEW
                     ARLDVDLTRMTASITCSRPEAGGHAQVWRSILD"
     gene            765..2009
                     /locus_tag="AC16CY_11155"
     CDS             765..2009
                     /locus_tag="AC16CY_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3354393.1"
                     /translation="MTHGAPVDRRNMAAIKGLTYVMFAMFAMTTDSVGLIIPEVIRTF
                     GLSLTAAGAFQYATMGGIALAGLLLGQLADRFGRRPILVAGLTLFAGASFAFAAGDTF
                     LFFTVLMGLSGVAIGVFKTAALALIGDISTSTAQHTAIMNTAEGFFGVGSIAGPAILA
                     RLLGAGVSWQWLYVLAGGICVLLIVTALQVRYPESGKPAARTGMSGTAAAVKDRFVLA
                     FSIGVFLYVAVEAAIYVWMPTLLGAGASRAWLVTYSLSIFFLLRAAGRFLGAWMLMRF
                     RWQAVLAVFSGGIFASFVLSVTMGAAWAVYLLPLSGLFMSVIYPTLNSKGISCLPRKD
                     HGAAAGVILFFTCVSAVLAPLAMGAVGDATGHIVYGFWLAAALAGLLFVGLLLNWVFN
                     PTRAVLDRLDSTDYGHEEPAST"
     gene            2165..2545
                     /locus_tag="AC16CY_11160"
     CDS             2165..2545
                     /locus_tag="AC16CY_11160"
                     /inference="COORDINATES: protein motif:HMM:NF047450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-PEP-CTERM-1 domain-containing protein"
                     /protein_id="HIR3354394.1"
                     /translation="MRTPRLVLFAAVALSMTAISVPSAQQSGGKKPPKQTPDKGVDDA
                     TASPGQERRTGERDIAAVTIVTRANGTTMAILDESFEDALVATVNPDGSITYKCVHGL
                     AAGSKHVASPAQPVSVPRQTAEEK"
     gene            2548..>4074
                     /locus_tag="AC16CY_11165"
     CDS             2548..>4074
                     /locus_tag="AC16CY_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HIR3354395.1"
                     /translation="MRALLRIVFAVCTLAALLTTTPAFANSTVTILNGNLPGVGFNDP
                     TPAAPVGGNAGTTLGAQRLIAFQHAANLWAARLDSAVEIRVLATFEALTCTATGAVLG
                     SAGTLFIFSDFPGASLANTWHHSALADKLALFELNPDPFLGFTPAPDIRARFNSSIGT
                     VPGCLTGTTWYHGLDANEAPNQIDLVAVVLHELGHGVGFSQFASVTSGAQPANLTDVY
                     GVNLLDTTTFKTWNQMTNAERAASAINSRHLVWDGPQVTADAPSVLAAGTPILRVTAP
                     AAIAGIYDVGTASFGPPLTSAGVAGQVVQALDVAEPAAPPLLAGTTTDGCSPILNAAA
                     IAGKIALVDRGLCGFVVKVKNAQNAGAIAVIVADNVAGSPPAGLGGADPTITIPSVRI
                     TLAAGNTIKPHLAGGVTGMLTLDLTLLSGADLSGRVFMNAPNPVQPGSSVSHWDTLAF
                     PNQLMEPAINANLTHSLVPPQDLTMSLLRDIGWYPDADVDGFADDVDECDASDLSPTV
                     V"
BASE COUNT          647 a         1364 c         1332 g          731 t
ORIGIN      
        1 aggttgcacg acaggtggac tgggtgtacg acttcgccct gcctccgctc gtgctgcaca
       61 ctctctacac ccgtgacgcc acgcccttga agcgatggct cgagatgcgg ccgcgcaacg
      121 cggtcaccgt actggatacg cacgacggga tcggtctgca ggacgccgga gcggacgatc
      181 ggcggccggg cgtgccaggc ctgcttccgg ccgaagccat tgacgcgctc ggggaaacga
      241 ttcaccagcg cagtggcggc gagagcaggc aggcaagcgg cactgcggca agcaacgtcg
      301 acgagcacca catcaactgc gcgttctacg atgcgctcgg ccgccgcgat gatgagtacc
      361 tcgtcgcacg cgcaatccag tgcttcgtgc ccggcattcc gcaggtctat tacgtggggc
      421 tgcttgccgg cggcaacgac atggagctgc tgcggcgaac gggcgtcggc cgtgacatca
      481 atcgccacta ctacacgggt gccgagctgc agcgggacct gagccggccc gtggttcgtg
      541 ccctgctcgc gctgctgcgg ttccggaaca gccacgcggc gttcgagggc gcttgtgacc
      601 ttgcgccgtc agccgggaac agcgtcgtcc tcgaatggcg gaacggtgcg gagtgggcga
      661 ggctcgacgt tgacctcaca cggatgacag cgtcgatcac ctgctcgcgg cccgaggcag
      721 gcggtcatgc acaggtctgg cgatcgatcc tggactgaac gaacgtgacg cacggcgccc
      781 cggtcgatcg ccgcaacatg gccgccatca aagggctcac ctacgtgatg ttcgccatgt
      841 tcgcgatgac gactgactcg gtggggctga tcattccgga ggtcatcagg acgttcgggt
      901 tgagcctgac cgcggcgggg gcgtttcagt acgcaacgat gggtgggatc gccctggccg
      961 gtctccttct cggccaactc gcagaccggt tcggccggcg gccgatcctg gtcgccgggc
     1021 tcacgctgtt tgcgggcgcc tcgtttgcgt tcgctgccgg cgatacgttc ctgttcttca
     1081 ccgtgctgat ggggctgtcc ggcgtcgcga tcggcgtatt caagacggcc gccctggcgc
     1141 tcatcggcga catctcgacc tcgacggcgc agcatacggc gatcatgaac acggccgagg
     1201 gcttcttcgg cgtcggatcg attgccgggc ccgcgatcct ggcccgcctg ctgggtgcag
     1261 gcgtgtcgtg gcagtggctc tacgtgctgg ccgggggcat ctgcgtgctc ctgatcgtca
     1321 ccgcgttgca ggtcaggtat ccggaatccg ggaagccggc ggcacgcacc ggcatgagcg
     1381 gtaccgcggc cgccgtgaaa gaccgcttcg tgctggcgtt ctccatcggt gtgttcctct
     1441 acgtggcggt cgaggccgcg atctacgtct ggatgccgac gctgctgggg gcaggcgcgt
     1501 cgcgcgcgtg gctggtgacg tacagcctct ccatcttctt cctcctccgc gccgcagggc
     1561 gttttctcgg cgcgtggatg ctgatgcgct tcaggtggca ggcagttctt gccgtcttca
     1621 gcggaggcat cttcgcgagc ttcgttctca gcgtgacgat gggtgcggcc tgggccgtct
     1681 atctgcttcc gttgtccggg ctcttcatgt ccgtgatcta tccaacgctc aattcaaagg
     1741 gcatcagctg cctgccccgg aaggatcacg gcgccgcggc cggagtgatc cttttcttca
     1801 cgtgcgtctc ggcggtgctg gcgccgctgg cgatgggcgc cgtcggcgat gcgacgggcc
     1861 acatcgtgta cggcttctgg ctggcggctg cccttgccgg cctcctcttc gtcggtctgt
     1921 tgctgaactg ggtgttcaat ccgacccgcg ccgttttgga ccggctggat tccacggatt
     1981 acggccacga ggaaccggca tcgacctgaa cgcgaccgtc aaccaaaagt gtaatctcgt
     2041 cattgccaac ggacagccca tccctggtta tcagcagttg atcacatgcg agggatggta
     2101 gtatcctccc cgctccaatc gaatctcgag aactgacatc cgttgcagaa accaggagga
     2161 cggaatgcgc accccccgtc tcgttttgtt cgccgcggtc gctctctcca tgacagcgat
     2221 cagcgtcccc agtgcccagc agtccggagg caagaaacca ccaaagcaga cgccagacaa
     2281 gggtgttgac gacgcgaccg cgtctccggg acaggagcga cgaaccggcg agcgcgacat
     2341 cgccgcggtg acaatcgtca cgcgggccaa cggcacgacc atggccatcc tggacgagtc
     2401 gttcgaagac gcactcgtcg caaccgtcaa cccggacggg tcaatcacct acaagtgcgt
     2461 gcacggcctg gcggccggca gcaagcacgt ggcatcgccg gcgcagccgg tcagcgtccc
     2521 gcgccagacc gcggaggaga agtagtcatg cgcgcgctcc ttcgcatcgt atttgcggtg
     2581 tgcaccctcg ccgcgctcct gaccacgacg ccggcgttcg cgaactcgac cgtcacgatc
     2641 ctcaacggta acctgccagg tgtggggttc aacgacccga cgccagcggc gcccgttggc
     2701 ggcaatgccg gcaccactct cggcgcgcag agattgatcg cgtttcaaca cgcggcgaat
     2761 ctctgggccg cgcggctcga cagcgccgta gagatacgcg tcctggcgac gttcgaggcg
     2821 ttgacgtgca cggcgacagg cgccgtcctc ggctcggcgg ggacgctctt cattttcagc
     2881 gacttcccgg gcgcgtcgct tgccaacacc tggcaccatt cggcgctcgc ggacaagctt
     2941 gccctcttcg agctcaatcc ggatccgttc ctcgggttca cgccggcgcc ggatatccgc
     3001 gcgcggttca atagcagcat cggaacggtg cctggctgtc tcacgggtac gacctggtac
     3061 cacgggctcg acgcgaacga ggccccgaat cagatcgatc tggtcgccgt cgtgctccat
     3121 gaactgggcc acggcgtcgg attctcccag tttgccagcg tcacgtccgg agcgcagccg
     3181 gccaatctga ccgatgtgta cggcgtgaac ctgctcgaca ccacgacgtt caagacctgg
     3241 aatcagatga ccaacgcgga gcgcgccgcc tcggcgatca actccaggca tctcgtgtgg
     3301 gacggccctc aggtgaccgc cgatgcgccc tccgtcctcg ccgcggggac cccgattctg
     3361 agagtcaccg cgccggccgc catcgcgggg atctacgacg tgggcacggc ctcgttcggc
     3421 ccgccgttga cgtccgcggg agtcgccggc caggtcgtcc aggcgctgga tgtggccgag
     3481 cctgccgcgc cgcctctgct ggcgggcacg accacggacg ggtgctcgcc gatcctgaac
     3541 gccgcggcga ttgcggggaa aatcgccctc gtggatcgtg gcctgtgcgg cttcgtcgtc
     3601 aaggtcaaga acgcccagaa cgccggcgcg atcgccgtca tcgtggcgga caacgtcgcg
     3661 ggaagccctc ccgcgggcct tggaggcgcc gatccgacca tcacgattcc atcggtgcgc
     3721 atcacgctgg ccgcgggcaa tacgatcaaa ccgcatctcg ccggtggtgt gaccgggatg
     3781 ctgacgctcg atctcaccct gctgtcgggc gcggacctct cgggccgggt cttcatgaac
     3841 gcgcccaatc ccgttcaacc gggctcgtcc gtgtcgcact gggacacgct ggcgttcccg
     3901 aaccagttga tggagccggc gatcaacgcc aacctcacgc actcgctcgt gccgccgcag
     3961 gacctcacga tgtcgctgct gcgtgacatc ggctggtatc cggacgcgga cgtggacggc
     4021 ttcgcggacg acgtggacga atgcgacgca tccgatctca gtccgacggt ggtc
//
LOCUS       DBNGSH010000482         1819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_45278, whole genome shotgun sequence.
ACCESSION   DBNGSH010000482 DBNGSH010000000
VERSION     DBNGSH010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_45278"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="AC16CY_11170"
     CDS             complement(<1..116)
                     /locus_tag="AC16CY_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3354396.1"
                     /translation="MSLVWFLIVGIVAGWLAGVLVKGGGFGVIGDLVVGVVGA"
     gene            complement(203..>1819)
                     /gene="ilvD"
                     /locus_tag="AC16CY_11175"
     CDS             complement(203..>1819)
                     /gene="ilvD"
                     /locus_tag="AC16CY_11175"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3354397.1"
                     /translation="LGLSDADMLKPKIAVVNSSSELAICFSHLDGIAKVVKEAVREAG
                     GLPFEIRTAAPSDFIISAGAGGGYMLASRDLIVNDMEIAIEGAQLDGIICLSSCDKTP
                     PAHLMAAGRFNIPSILVICGYQPSGHYEGKHVDIEEVFIGSVQAMFGKLSKDSLAGMC
                     DNAITGPGVCAGMGTANSMHIVCEALGMTLPGAAPVLANSPKMFDLARQSGRRIVEMV
                     WEDLTPRQILTPGAFRNAAACVLAVSGSINCVKHLQATAVEAGVDVDVFGLFNDLGRQ
                     VPVSSAVAPNGPDTIDAFEAAGGARALLKQLSTLLDLDALTVTGRRLRDNLAEAVVHN
                     ADVIRPLERPFARHASIVVLRGTLAPDSAIIRPGLHRPGVDGRFEGTALVFDGVPEAI
                     AAVQRGELTRGQVLVLRGCGPKGGPAMAGSASRVVFAIYAAGLENDVAFVSDGQLSGL
                     CNKGMTVAEVSPESAVGGPLALVENGDRIRIDIDGRSLDLDVAEAELTARRARRGDVA
                     LPRSTGYLSIYQRSVQPMSTGAVLVEKGRR"
BASE COUNT          303 a          652 c          591 g          273 t
ORIGIN      
        1 gcgccgacga caccgacgac gaggtcaccg atcactccga agccgccgcc cttgaccagg
       61 acgccggcca gccagccggc cacgataccc acgatgagaa accacaccag gctcatgtca
      121 gtctcccttc ttcgcgagaa cgatgacgac gaggagccag agtctccccc gaaccggccc
      181 gccaatcaag gtcagcccaa gattagcgcc ggcccttctc gaccagcacg gcgccggtgg
      241 acatcggctg aacgctccgc tgatagatcg agaggtaccc ggtcgagcgg gggagggcca
      301 cgtcgccgcg gcgtgcccgt cgtgccgtga gctccgcctc cgcgacgtcg agatcgagcg
      361 agcgtccgtc gatgtcgatg cgaatgcgat cgccgttctc gacgagcgcg agcggtccgc
      421 cgacggccga ttcgggcgac acctccgcga ccgtcatccc cttgttgcag aggcccgata
      481 gctggccatc ggacacgaac gcgacgtcgt tttcgaggcc ggcggcgtag atggcgaaca
      541 ccactcgcga cgccgacccg gccattgccg gtccgccctt cggaccgcag ccgcgaagga
      601 cgaggacctg cccccgtgtc agctcgcccc tctgcaccgc cgcgatcgcc tcagggacac
      661 cgtcgaagac gagcgccgtg ccctcgaagc gtccgtcaac gcccggccga tgcaggccgg
      721 gccggatgat cgcgctgtcc ggcgcgagcg tgccgcgcag cacgacgatc gacgcgtggc
      781 gcgcgaacgg ccgttcgagc ggccggatca cgtccgcgtt gtgcaccacg gcttccgcca
      841 ggttgtcgcg gagcctccgc cccgtcaccg tgagcgcgtc gaggtcgagc agcgtggaga
      901 gctgcttcag cagcgcccgc gcgccgccgg ccgcctcgaa ggcatcgatc gtgtcggggc
      961 cgtttggcgc gaccgcgctc gacaccggca cctgccgccc gaggtcgttg aaaagaccga
     1021 agacatccac atcgacgccg gcctctacgg cggtcgcctg caggtgcttc acgcagttga
     1081 tcgacccgct gacggccagc acgcacgccg cggcattgcg aaaggcgccc ggcgtcagaa
     1141 tctgccgcgg cgtgaggtcc tcccacacca tttccacgat gcggcgtccc gactgacgcg
     1201 ccaggtcgaa catcttcggg ctgttggcga gcaccggcgc tgctccggga agcgtcatgc
     1261 ccagcgcctc gcagacgatg tgcatggagt tggcagtgcc catgccggca cagacgccgg
     1321 ggcctgtaat cgcgttgtcg cacatgccgg cgagcgagtc cttcgacagc ttgccgaaca
     1381 tcgcctggac cgagccgatg aacacctctt cgatgtccac gtgcttgccc tcgtagtggc
     1441 cgctcggctg gtagccgcag atgacgagga tcgacgggat gttgaagcgt ccggccgcca
     1501 tgaggtgagc cggcggcgtc ttgtcgcacg acgacaggca gattatcccg tcgagctgcg
     1561 cgccctcgat cgcgatctcc atgtcgttga cgatgaggtc gcggctggcc agcatgtagc
     1621 cgccgccggc gcccgcgctg atgatgaaat cgctcggcgc cgccgtccgg atctcgaacg
     1681 gcagcccgcc ggcctctcga acggcctcct tcacgacctt cgcgatgccg tcgagatgac
     1741 tgaagcagat ggccagctcc gaggagctgt tcacgacggc gatcttcggc ttgagcatgt
     1801 cggcgtcgct cagcccgag
//
LOCUS       DBNGSH010000483         2114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3101844, whole genome shotgun sequence.
ACCESSION   DBNGSH010000483 DBNGSH010000000
VERSION     DBNGSH010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3101844"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="AC16CY_11180"
     CDS             complement(<1..398)
                     /locus_tag="AC16CY_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354398.1"
                     /translation="MPPVTIIDNPYATLWYHPEKRIVHHQIHQFISGDTFRELLLAGT
                     NVLIKHHAQKWLSDDRANAVLRPEDVEWSHEFWFPQTAKAGWKYWAIVQPVKTVGQVT
                     MKGLASTYGSHGITSKNFTDPSDAMWWLES"
     gene            596..>2114
                     /locus_tag="AC16CY_11185"
     CDS             596..>2114
                     /locus_tag="AC16CY_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3354399.1"
                     /translation="MAFKQLLRGSLGDPRDPGPPKDQGRPRTAVLYLTADARESRLVN
                     GAFNFAHPHLQLDLSVALKDARAHLAQPGKYDALVIGWSVPESDGASLIAHVREQRYP
                     LAVVASGESLDSYREAGADQCVPRGGSFLARLPVAIEEAVRHRRVASPAPAPPASPAP
                     AAGKGLRIAFAGDLDQFKADLTTAASPLQVVPLSQALAEADVRAPNAALDFDLVLIDH
                     GAPNTNTASVLADVRARNLSVPIVLLVSPMEERSALQIFGPTVDEYLAKTTGWLARMS
                     IRLDGVLARQAQVRELAALRVKEARLRSMVDRLPACILRLSVEGTVLAGNAVALSMLN
                     AASPNALVRKPFETFVRDEDRNQWSDFFTRVCGGEQRSIEVRVSTVDGTQRVLEVSAV
                     PVPAELGRDSSVLMVLRDVSDRKRLEAAVEQITTNSDAGAAAATASGPDPALLRELES
                     DLHRLSGQARTTFEELGALLRNAEARHDEAFARQADEYARLKAAQLEHWRSYEEFV"
BASE COUNT          330 a          722 c          701 g          361 t
ORIGIN      
        1 tgcgactcga gccaccacat cgcgtcggac gggtcggtga agttcttcga cgtgattccg
       61 tgcgagccgt aggtggacgc caggcccttc atcgtcacct gaccgaccgt cttcacgggc
      121 tgcacgatcg cccagtactt ccagcccgcc ttcgccgtct gcgggaacca gaactcgtgg
      181 ctccactcca catcctcggg gcgcagcacc gcgttggccc gatcgtcgga cagccacttc
      241 tgcgcgtggt gcttgatcag cacgttggtc ccggccagca gcagctcgcg gaaggtgtcg
      301 ccgctgatga actggtgaat ctggtggtga acgatccgct tctcagggtg gtaccagagc
      361 gtcgcgtacg ggttgtcgat gatcgtcacc gggggcattg cgttggactc ccgaaaccgc
      421 tgttgacgcc tcgattctgc ggttgggtta tcggcagcgc cgaaaggcga ctgtaacgag
      481 gccgacactc gtgtgaggga gtgtgccgac acgcgcaact gactgactgg tatggccgaa
      541 acggaccaac ctgagctata tcgttttttt gtgacaccgc cgacacgtac cgtggatggc
      601 gttcaagcag ctcttgcggg ggtcgctggg cgaccccagg gatcccggtc cgccgaagga
      661 tcagggcagg ccgcgtacgg ccgtcctcta cctcacggcg gacgctcgcg agagcaggct
      721 ggtgaacggc gcgttcaact tcgcgcaccc tcaccttcag ctcgacctgt cggtcgcgct
      781 gaaagacgcc cgtgcccatc tcgcacagcc ggggaaatac gacgcgctcg tgatcgggtg
      841 gagcgtgccg gagagcgacg gcgcgtcgct gatcgcgcac gtccgcgagc agcgatatcc
      901 gcttgccgtc gtcgcttcgg gcgagtcgct cgactcgtat cgcgaggccg gcgccgacca
      961 gtgcgtgccg aggggcgggt cgttcctggc acgcctgcct gtcgccatcg aggaagcggt
     1021 caggcacagg cgtgtcgcct cgccggcgcc cgcccctccc gcatcccccg cgcctgccgc
     1081 cggtaaaggc ctgcgcatcg ccttcgccgg agacctggac cagttcaagg ccgacctgac
     1141 gaccgcggcg tcgcccctgc aggtcgttcc cctgtcgcaa gcgctcgcgg aggcggacgt
     1201 ccgggccccg aacgccgcgc tcgacttcga tctcgtcctc atcgatcacg gcgcgccgaa
     1261 cacgaacacc gcctcggtgc tcgccgacgt tcgcgcgcgg aatctcagcg tgcccatcgt
     1321 gctgctcgtc agcccgatgg aagagcggtc ggcgctccag atcttcgggc caaccgtaga
     1381 cgagtatctc gcgaagacga ctggctggct cgcgcgcatg tccatacggc tggacggcgt
     1441 cctcgcgcgc caggctcagg ttcgcgagct ggccgccctt cgcgtcaagg aagcgcggct
     1501 ccgctcgatg gtggaccggc tgcccgcctg cattcttcgg ctctcggtgg agggcaccgt
     1561 gcttgccggc aacgcggtcg ccctctcgat gctgaacgcc gcgagtccca acgcgctcgt
     1621 gcgcaagccg ttcgaaacct tcgtccgcga cgaagaccgc aatcaatgga gcgacttctt
     1681 cacgcgcgtc tgcggcggcg agcagcgctc gatcgaggtt cgtgtctcga cggtggacgg
     1741 aacacagcgg gtcctcgaag tgtccgccgt gccggtcccg gccgagctcg gccgggactc
     1801 ctccgtgctg atggtgctcc gtgacgtgag cgaccggaag cggctagagg ccgcggtcga
     1861 acagatcacg acgaattccg acgccggtgc ggccgccgcc acagcgtccg gtccggaccc
     1921 ggctctcctt cgcgagctcg aaagcgatct tcatcgcctg tcgggtcagg ctcgcacgac
     1981 gttcgaggag ctcggcgcac tcctgcggaa tgcggaagcg cggcacgacg aggcgttcgc
     2041 ccggcaggcc gacgaatacg cgaggctcaa ggcggcgcaa ctcgagcact ggcggtcgta
     2101 cgaggagttc gtgc
//
LOCUS       DBNGSH010000484         6091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_894119, whole genome shotgun sequence.
ACCESSION   DBNGSH010000484 DBNGSH010000000
VERSION     DBNGSH010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_894119"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC16CY_11190"
     CDS             <1..375
                     /locus_tag="AC16CY_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3354400.1"
                     /translation="PGQPPAAGAGRGNLASRFTGKIEVGTTTGTNMSRIRFEAGARTN
                     WHVHTAGQLLLVEEGRGRLQELGSDIVDIPVGQPVLTKPNLLHWHGAAPDQAALQFSV
                     YSGDLEWKEAVTDDQYLGKAKR"
     gene            372..2084
                     /locus_tag="AC16CY_11195"
     CDS             372..2084
                     /locus_tag="AC16CY_11195"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR3354401.1"
                     /translation="MRAIGTRLQAQGERLRTRLAAAALAVVVAVSFVAGQQSSDTRLP
                     PLPPPKLPLVTVQDLRDGLKNPTRWLMYSGDYTGQRHSPLKQITPDNVRRLAAQWTFQ
                     AEGMAINRGYETTPLILDGVMYVTGNNNLAWAVDMKSGRLIWRYRRALPAGLTYGGAN
                     PSNRGFAALGDRLFMGTVDAHLIALNRNTGEVVWDVPVEDYKVGHAITAAPIVVKDKV
                     ITGNAGGDLPTRGFIDAYDAATGKRAWRFYTIPAVGEPGSETWSDPAVLPRGGGATWM
                     TGSYDPELNLIYWGTGNPNPDYYGGDRQGDNLYTASLVAVDADTGKLRWHFQFTPHDL
                     HDWDSTHVPVLADLTVAGQPRKVVMVANRNGFFYVLDRVTGKLISGKPFTGTKWAREL
                     GPDGKPIVLNSGAMMPGDTKEPQCVPDLRGGTNYNPPSYDPGLELFFVMARESCAIYT
                     PVKQEMVPARSFMSGGMKKLPDPDYSAVRAIDPKSQTIKWEYKLPTPSLAGVMSTATG
                     LVFAGDNEGNFNAFDSRTGKLLWTYRMGSLIWGAGATSVMLDGRQLVLLPSGNTIVAF
                     GLPE"
     gene            2141..2965
                     /locus_tag="AC16CY_11200"
     CDS             2141..2965
                     /locus_tag="AC16CY_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3354402.1"
                     /translation="MLAVLLCAVPASAQLAEPNAAGVSMGHLHYQVRDVEANKRFWMA
                     LGGQPAAAGDAVIVKFPDVIVFLAAAPSSGGTEGSVLNHVAFRVPSLSSVEAAGLSVA
                     RLAQFPGVASVRSPEGERIELFENAATNLTFTQDAGHSDTAAERHNRPLASPVAFHHV
                     HLYLPSGQVGEAKAWYTRMFGGVPGKRSNYDAVDLPGINLNFSEAPKPVVPTSGRMLD
                     HIGFEVRGLEAFCRRLQSMGVIFDRPYGKDGSGIASARLTDPWGTSIELTEGLARL"
     gene            2985..3938
                     /locus_tag="AC16CY_11205"
     CDS             2985..3938
                     /locus_tag="AC16CY_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354403.1"
                     /translation="MTTRTPLIAAAAAVFVWAASSTVVGQHAAPRTPWGDPDLQGTYT
                     NTYENGTPLERPDQFAGRRLEDVKGEELASIRREIQKRTIGQFLGPIHAPENWWQDNL
                     YLDRGSQAWFVIDPPDGKVPPTTPEAQRRAAARAAARKASTRGPADSYEDRSLYDRCI
                     TRGLPGSMMPAIYGNQYQIVQAPGYVAILYEMIHETRVIPLDGRAQLGTRIVQDMGDA
                     RGRWDGDTLVVTTTNFKERSVYRNANPDTLTLVERFQRVSPDKVRWTVTVDDPATWTR
                     PWTFSLPLTADPNPLPLYECHEGNYGLKNILSAARAEERAR"
     gene            3975..5342
                     /locus_tag="AC16CY_11210"
     CDS             3975..5342
                     /locus_tag="AC16CY_11210"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3354404.1"
                     /translation="MSETHALASYIVSNCWQDIPDDVRHEAKRAILNFVGCAIGGSPH
                     PAVTTAIRALGPFSGDRTASVLGRSERLDPLHASLMNGISSHVEDYDDTTPKNYSHTS
                     SPVASALFAYASVTGVRGRDFVEAFVLGFEAASRAGNAVYPSHYDVGWHMTGTIGVFG
                     AAAAIGKLIGLSVEQMVWALGLAATQSAGLREMFGSMGKAFHPGRSAQNGYAAALLAQ
                     AGFTAGERGIEGPRGFAHVLAASRDLSKITARLGADFDLRENTYKPFPCGIVNHPTID
                     GAIQIHDEERPDPASIAAVRLRVAPLVLDLCNQQDITRGLQGKFSVYHGAAVGLVRGK
                     AGLREYTDEAVNDPAIKRVRELTTAVGDASVTEDQSRIEVELKDGRRLTRFVEQSLGN
                     IHRPLSDTQLEDKFRDQAVMVLPAGDVDALIQSCWRIDQLDDVRDLVTLAVPLSARAA
                     SSVRL"
     gene            5399..>6091
                     /locus_tag="AC16CY_11215"
     CDS             5399..>6091
                     /locus_tag="AC16CY_11215"
                     /inference="COORDINATES: protein motif:HMM:NF019533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HIR3354405.1"
                     /translation="MKLTATTAVIIASTLVACSRWPDPPAVDQATYKAGHATWLADQD
                     ALVSEVLPIVGIWPLAEGETAFGADADLPIVLPAAGTGKAGVFHRQGSTITVVPAAGA
                     ALRTADGKPIDQATEVSEEVWLGSLRMSIAGAPDGRLWVSASDEQHPAVKNPPTVEAY
                     PLDPRWRIAARFDAFDSPKPVRVPDVRGGTMDFTAMGQLVFRVNGQEMRLTALGEPGS
                     EQFFVMFKDPTNQ"
BASE COUNT         1045 a         2080 c         2024 g          942 t
ORIGIN      
        1 ccgggacagc cgccggcggc gggcgcaggg cggggcaacc tagcgtcgcg cttcaccggc
       61 aagatcgaag taggcaccac gacgggcacg aacatgtcgc gcatccgctt cgaggccggc
      121 gcgcgaacga actggcatgt ccacacggcg ggtcagttac tgctggtcga ggaaggacgc
      181 ggacgcctgc aggagttggg aagcgacatc gtcgatatcc ccgtcgggca gcccgtgctc
      241 accaaaccaa acttactgca ctggcacggc gccgctcctg atcaggccgc gctgcagttc
      301 agcgtctaca gcggggacct cgaatggaag gaagcggtta ccgacgatca gtacctgggg
      361 aaggcgaaga gatgagggcg ataggcacaa ggctccaagc gcaaggcgaa agacttagga
      421 cgcgacttgc cgcggcggcc ctggccgtcg tcgtcgccgt ttcgttcgtc gccggacagc
      481 agtcatcgga cacgcgcctt ccgccgctgc cgccgccgaa gctcccgctc gtgacggtgc
      541 aggatctgcg cgacgggctg aagaacccga ctcgctggct gatgtactcc ggcgactaca
      601 cggggcagcg ccacagcccg ctgaagcaga tcacgccaga caacgtccgg cgcctggccg
      661 cgcagtggac gtttcaggcc gagggcatgg cgatcaaccg cgggtacgag acgacaccgc
      721 tcatcctcga cggcgtgatg tacgtcaccg gcaacaacaa ccttgcatgg gccgttgaca
      781 tgaagagcgg gcgcctgatc tggcgctacc ggcgcgcgct ccccgccggc ctcacctatg
      841 gcggcgcgaa tccctcgaat cgcgggttcg ccgcgcttgg cgaccggttg ttcatgggta
      901 ccgttgacgc gcatctgatc gctctcaaca ggaacaccgg cgaggtcgtg tgggacgtgc
      961 ccgtcgaaga ctacaaggtc ggccatgcga tcaccgccgc gccgatcgtc gtgaaagaca
     1021 aggtgatcac cggtaacgcg ggcggcgacc tgccgacgcg cggcttcatc gatgcctacg
     1081 atgcggcgac gggcaagcgc gcctggcgct tctacacgat ccccgcggtc ggcgagccgg
     1141 gcagcgaaac ctggtcggat cccgccgttc tcccgcgcgg cggcggcgcg acgtggatga
     1201 caggcagcta cgatcccgag ttgaacctga tctactgggg aaccggcaat cctaatcccg
     1261 attactacgg cggcgatcgc cagggcgaca acctctatac ggcgtcgctc gtcgccgtgg
     1321 acgccgatac cgggaagctc cggtggcatt ttcagttcac gccgcacgat ctgcacgact
     1381 gggactcgac ccacgtaccc gtgcttgcgg atctcaccgt cgccggccag ccgcgcaagg
     1441 tcgtgatggt ggccaaccgc aacggtttct tctacgtcct cgaccgcgtc acgggaaagc
     1501 tcatttcagg gaagccgttc accggcacga agtgggcgcg cgagctcggg ccagacggca
     1561 agccgatcgt gctgaacagc ggcgccatga tgccgggcga cacgaaggaa ccgcagtgcg
     1621 tccccgactt gcgcggcgga accaattaca acccgccgtc gtacgatccc ggccttgagc
     1681 tgttcttcgt gatggcccgg gagtcgtgcg cgatctacac gccggtcaag caggagatgg
     1741 ttcccgcccg ctccttcatg agcggtggga tgaagaagct gcccgacccc gattacagcg
     1801 ccgtccgcgc catcgatccg aagagccaga cgatcaagtg ggagtacaag ctgccgaccc
     1861 cgtcgctcgc gggcgtcatg tccacggcca cgggcctcgt ctttgccggc gacaacgagg
     1921 ggaacttcaa cgcgttcgac tcgcgcaccg ggaagctgct gtggacgtac cggatgggat
     1981 cgctcatctg gggggccggg gccacgagcg tcatgctcga cggacgtcag ctcgtcctgc
     2041 tgccgtccgg gaacaccatc gtcgcgttcg gcctgccaga gtgatgcgcg cggtagggcg
     2101 gccggatcct cgcaagacac gactgctgtc cgccggttcg atgctggccg ttcttctgtg
     2161 cgccgttccc gcgtcggcgc agctggcgga gcccaacgcc gccggtgtct cgatgggaca
     2221 cctgcactac caggtccgcg acgtcgaggc gaacaagcgg ttctggatgg cgctcggcgg
     2281 gcagcccgcc gctgccggcg acgccgtcat cgtgaagttc ccggacgtga tcgtgttcct
     2341 cgcggcagcg ccttcgtcgg gcggcacgga aggatcggtt ctcaatcacg tcgccttccg
     2401 ggtgccgtcg ctctcgtcgg tcgaagccgc cgggctgtcg gttgcgcgcc tcgcgcagtt
     2461 cccgggcgtg gcgtccgtcc gatcgcctga aggcgagcgg atcgagctgt tcgagaacgc
     2521 ggccacgaac ctgaccttca cccaggacgc aggccatagc gacaccgcag ccgaacggca
     2581 caatcggccg cttgcctcgc cggttgcctt ccaccacgtg cacttgtacc ttccatccgg
     2641 acaggtcggc gaagccaagg cgtggtacac gcgcatgttc ggcggcgttc cgggcaagcg
     2701 ttcgaactac gacgcggtcg atctgcccgg gatcaacctg aacttcagcg aggcgccaaa
     2761 gccggtcgtg cccacgagcg gccggatgct ggatcacatc ggcttcgagg ttcgcggcct
     2821 cgaagcgttc tgccggcgcc tccagtcaat gggtgtaatc tttgatcgcc cgtacgggaa
     2881 agacgggtcg ggcatcgcaa gcgcgaggct caccgacccg tgggggacgt cgatcgagct
     2941 gactgaaggg ctcgcacgcc tgtagctcct gaatcggagt gatcatgacg actcgcacgc
     3001 cactcatcgc tgccgctgcg gccgtgttcg tctgggcggc gtcgtccacc gtggtgggcc
     3061 agcacgccgc gccccggact ccgtggggcg atcccgatct gcagggcacc tacaccaaca
     3121 cctacgagaa cggcacgccg ctcgagcggc cggaccagtt cgcgggacgc cggctggagg
     3181 acgtcaaggg agaagagctc gcctccatcc gccgggaaat tcagaagcgc acgatcgggc
     3241 agttcctcgg ccccatccac gctccggaga actggtggca ggacaacctc tatctcgatc
     3301 gcggcagcca ggcgtggttc gtcatcgatc cgcccgacgg caaggtcccg ccgaccacgc
     3361 ctgaagcgca gcgtcgcgcc gccgcgcgag cggcagcccg caaggcgagc acgcgcggtc
     3421 cagccgactc ctacgaggat cgttcgctct acgatcgttg catcacacgc ggcctgccgg
     3481 gctcgatgat gccggcgatc tacggcaacc agtatcagat cgtccaggcg ccggggtacg
     3541 tggcgatcct ctacgagatg atccacgaga cgcgcgtcat cccgctcgac ggacgcgcgc
     3601 agctgggcac gcgcatcgtg caggacatgg gcgacgcccg cggacggtgg gacggcgaca
     3661 cgctggtcgt gaccacgaca aacttcaagg agcgcagcgt ctaccgcaac gcgaacccgg
     3721 acacactgac gctcgtcgaa cggttccagc gcgtcagccc ggacaaggtt cggtggacgg
     3781 tgacggtgga cgaccccgcc acgtggacca ggccgtggac cttctcgctg ccgctgaccg
     3841 ccgacccgaa tccgctgccg ctctacgagt gccacgaagg caactacggc ctgaagaaca
     3901 tcctgagcgc cgcgcgcgcc gaggaacgcg cgaggtaacc gtagcgcgcc ccctttcggg
     3961 gggcgcgacc acacatgtct gaaacccacg ccctcgcctc ctacatcgtt tcgaattgct
     4021 ggcaggatat ccccgacgat gtccgtcacg aggcgaagcg cgcgatcctg aacttcgtcg
     4081 gctgcgcgat cggcggcagc ccgcatccgg ccgtgacgac ggccatccgc gccctcggcc
     4141 ccttctcggg cgaccgcacg gcgtcggtgc tcggccgatc cgagcggctc gacccgctgc
     4201 acgcgtcgct gatgaacggc atcagctcgc acgtcgagga ctacgacgac acgacgccga
     4261 agaactacag ccacaccagc tctccggtgg catccgcgct ctttgcctac gcgagcgtta
     4321 cgggggtgcg gggccgcgac ttcgttgagg cgttcgtcct cggtttcgaa gccgcctccc
     4381 gcgcgggaaa cgccgtctac ccgtctcact acgacgtcgg atggcacatg accggaacga
     4441 tcggcgtgtt cggcgcggcc gccgccatcg gcaagctgat cgggctgtct gtggagcaga
     4501 tggtctgggc gctcgggctc gcggcgacgc agtccgcggg cctgcgcgag atgttcggct
     4561 cgatgggcaa ggcgtttcac ccgggacgct cggcgcagaa cggttacgcc gcggcgctgc
     4621 tggcgcaggc gggtttcacg gccggcgagc gaggcatcga gggcccccgg ggcttcgcgc
     4681 acgtcctcgc tgcctcacgc gacctgtcga agatcacggc gcgcctcggc gccgatttcg
     4741 acctgcgcga gaacacctac aagcccttcc cgtgcggcat cgtgaaccat ccgacgatcg
     4801 acggcgccat ccagattcac gacgaggagc gcccggatcc ggcgtcgatc gccgccgtcc
     4861 gcctgcgcgt cgccccgctc gtcctcgacc tgtgcaatca gcaggacatc acgcgcggcc
     4921 tgcaggggaa gttctccgtc taccacggcg ctgccgtcgg gctggtgcgg ggcaaggcgg
     4981 gccttcggga gtacaccgac gaggcggtga acgacccggc gatcaagcgc gtgcgcgagc
     5041 tgacgacggc cgtcggcgac gcctcggtca ccgaggacca gtcgcgcatc gaggtggagc
     5101 tgaaggatgg ccgccggctc acccggtttg tcgagcagtc gctcggcaac atccatcgtc
     5161 ccctgagcga cacgcagctc gaggacaagt tccgcgacca ggccgtcatg gtgctgcccg
     5221 cgggggacgt ggacgcgctc atccagtcgt gctggcgcat cgatcaactg gacgatgtgc
     5281 gagacctggt gacgctggca gtgccgctct ccgcgagggc ggccagtagt gtccggcttt
     5341 agccggacct gttgacgtag tgtccggctt tagccggacc cgtgaccgac caagagacat
     5401 gaaactcacc gccacgaccg ccgtcatcat tgcttcgact ctcgtcgcgt gcagccgctg
     5461 gcccgatccg cctgccgttg accaggccac gtacaaggcc gggcacgcca cgtggctcgc
     5521 cgatcaggac gcactcgtct ccgaggtgct tcccatcgtc ggtatctggc cgctcgcgga
     5581 gggcgagacg gcattcggag cggacgcgga tctgccaatc gtcctgcccg cggctgggac
     5641 aggaaaggcc ggcgtctttc accggcaggg atcgacgatc accgtcgttc cggcagcggg
     5701 cgccgcactg cggaccgccg acggaaagcc catcgatcaa gcgacggaag tcagcgaaga
     5761 agtctggctg ggctcgttgc ggatgtccat cgcgggcgca ccggacggcc gcctctgggt
     5821 gagcgcgagc gacgagcagc atccggcggt caagaatcca ccaaccgtcg aggcgtatcc
     5881 gctcgacccg cgctggcgga tcgccgcacg cttcgacgcg ttcgactcgc cgaagcccgt
     5941 gcgcgttccc gacgtgcgcg gcgggaccat ggatttcacc gcgatgggcc agctcgtgtt
     6001 ccgcgtgaac gggcaggaga tgcgcctcac cgcgctgggc gagccgggca gcgagcagtt
     6061 cttcgtgatg ttcaaggatc cgaccaacca g
//
LOCUS       DBNGSH010000485         4282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4459473, whole genome shotgun sequence.
ACCESSION   DBNGSH010000485 DBNGSH010000000
VERSION     DBNGSH010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4459473"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC16CY_11220"
     CDS             <1..311
                     /locus_tag="AC16CY_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354406.1"
                     /translation="KGTYLELGLGGPGFSRSKWSLTVPVRVGLSLGDYYESLAGDDTF
                     GFLSVAGVVTIPLSSAPTKVGTWNIHGGVEYLLLGDRNEAVYGDSSHVIGSVGIGLSY
                     "
     gene            418..2943
                     /locus_tag="AC16CY_11225"
     CDS             418..2943
                     /locus_tag="AC16CY_11225"
                     /inference="COORDINATES: protein motif:HMM:NF019935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase family
                     protein"
                     /protein_id="HIR3354407.1"
                     /translation="MALTEPGRGGSTVVAAFGAARSELEADVRRGAGGRAALERYSDR
                     IDAMVRQLFAEAGGAREQAVVLALGGYGRRHLCLHSDIDLLVLFGGRIGAEQEGFLRA
                     FLNPLWDLGVIVGHQVRELDDFARLEADNPEFLLALLDSRPVAGLRVLFDRFGAAFHT
                     ADTHAHILRSLLELVDDRHARFNSTLYQLEPDVKDAPGALRDLMAARTIALLTDPVLL
                     RRGPADPARVDQAEDFLLRVRSTLHLDAGRNQNTLSHEMQERTADLLGYPGAEPRQQV
                     ERLMSDYFRHARTVHRSLAWARKGAPVPVGPNLGVSRDGIRFLDPIQAARNPASWIGA
                     FHAAIETGVDVSEEALSCIQQHVERFRADDFFPDARDRSALLSLLKPRPGLYDRLSEM
                     HDCGLLGRVFPEFQAISWRVVRDFYHKYTVDEHTLLTIRNLERISTTDVPYRIRFRNL
                     LGEVDRPELLVLALLLHDVGKWRDDDHALESVRMATEALERLELDAGARETVLFLIRH
                     HLRMSLVAFRRDTEDPDIVKEFAGFVGTEDRLKMLCLLTLVDVEAVSPETLTPWKEEL
                     LWRLYVDTYNHLTQRYGDELIERNQAGLDDLLKQRPADLSVPEITRFLEGLPQRYLQL
                     FPRDAIYRHVRLASDIHPDEVHASLERGEDAVWSLAVATLDKPFLFSNICGVLSSFGM
                     NIIRGHALTNPNGLVLDVFQFTDDERYLELNRDAQEQVVHVLEDVVSARADVAERLRP
                     REQGVLHVKGAPRFPPVVRADNHASGRYTIVDIVASNALGLLYRISRVISRHGCDVDL
                     VLIATEGERAIDVFHITKGGSKLTEAEQQALTSDLYRTLEGSI"
     gene            2940..3269
                     /locus_tag="AC16CY_11230"
     CDS             2940..3269
                     /locus_tag="AC16CY_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3354408.1"
                     /translation="MKLIKCIIRPNKVDEVKDALAKLSIAGMTVTEVRGHGKQKGHTA
                     IYRGKEYNVSLLPKMEIEIVVPDNMADDVVKAVVQAARTGEIGDGRVFVLPVLESYRI
                     RTGEKES"
     gene            3333..>4282
                     /locus_tag="AC16CY_11235"
     CDS             3333..>4282
                     /locus_tag="AC16CY_11235"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3354409.1"
                     /translation="MSSHRIPFEASEWAEYSRLQADNDRLRHLLLEQHRAADLAGHSV
                     AIRRVRELIAQVAPSPHAVCLRGEPGVGKSRAARAIHAASSRSAAPFVTVACAAMPDD
                     VADDQLFGPGWAETSPRGPAAGCVGRAAGGTLFFDEVAALGPLAQRRLASLARDRAVT
                     LAGRRVPVDVRLIAASSRNIDELTASGVFGRDLRDEIAVVAIPIPALRDRKVDLPLLA
                     DLFVELFSRTHVRAVRSVSGRAMDVLMNYGWPGNVTQLRQAIEWAGAAAAGPVIHHHH
                     LPAEIQAAGESVGPFAPGLGAALEAYERELLQDALSRARGV"
BASE COUNT          653 a         1432 c         1489 g          708 t
ORIGIN      
        1 acaaggggac gtacctcgag ctcggtttgg gggggccagg cttctcgcgg tcgaagtgga
       61 gcctgacggt tccagtcagg gtcggcttga gcctcggcga ttactacgag agcctggctg
      121 gtgatgacac gttcgggttc ctgagcgtcg ccggggtcgt gacgatcccg ctctccagcg
      181 caccgacgaa agtcggtacg tggaatatcc acggcggggt cgagtacctg ctgctgggcg
      241 accggaacga ggcggtctac ggcgacagtt cgcatgtgat tggttccgtc ggcatcggtc
      301 tcagctacta gcggcgccgc cgtgtcgccg cggaagaccc acgggacgcg cgcgtctcgt
      361 cctgtgtcac gtccacttgc gcgtatgctt gagaccacga cgggagacga tcacacgatg
      421 gcactcaccg agccgggccg cggcgggtcc acggtcgtcg ccgccttcgg ggccgctcga
      481 tccgagctcg aggccgacgt gcggcgcggg gcgggcggcc gcgcggcgct cgagcgctac
      541 tccgatcgca tcgacgcgat ggtgcggcag ctcttcgccg aggccggcgg cgcgcgcgaa
      601 caggccgtgg tgctcgcgct cggcgggtac ggccggcggc atctctgcct gcattcggac
      661 atcgacctgc tggtgctgtt cggcgggcgc atcggggccg aacaggaagg gtttctccga
      721 gcgtttctca acccgctgtg ggacctcggc gtcatcgtcg ggcaccaggt gcgggagctc
      781 gacgacttcg cgcggctcga ggcggacaac ccggagttcc tgctggcgct cctcgactcg
      841 cgcccggtcg ccgggcttcg cgtcctgttc gaccggttcg gggccgcgtt tcacactgct
      901 gatacgcacg cccacatcct gaggtcgctg ctcgagctcg tggacgaccg gcacgcgaga
      961 ttcaactcga ctctgtacca gctcgagccg gacgtgaagg acgcgccggg ggcgcttcgc
     1021 gacctgatgg ccgcgcgcac catcgcgctg ctgaccgacc ccgtgctcct gcggcgcggc
     1081 cccgccgacc cggcgcgcgt ggaccaggcg gaagacttct tgcttcgcgt tcgatcgacg
     1141 ctgcacctgg atgcgggacg gaatcagaac accctcagcc acgagatgca ggaacggacg
     1201 gcggacctcc tcggctatcc aggcgccgag ccccgccagc aggtcgagcg gctgatgagc
     1261 gattacttcc gccacgcgcg tacggtccac cggtcgctcg cctgggcgag gaagggcgcg
     1321 cccgtgccgg tggggcccaa tctgggcgtg tcgcgcgacg gcatccgctt cctcgatccg
     1381 attcaggcgg cgagaaaccc ggcctcgtgg atcggcgcgt tccatgccgc gatcgagacg
     1441 ggcgtcgatg tgtccgagga ggccttgtcg tgcatccagc agcacgtcga gcggttccgg
     1501 gccgacgact tcttccccga tgcccgcgac cggagtgcgc tgctgtcgct gctcaagccg
     1561 cgtccgggat tgtacgaccg gctgtccgag atgcacgact gcgggctgct cggccgcgtc
     1621 tttcccgagt tccaggccat ttcctggcgc gtggtgcgcg acttctacca caagtacacg
     1681 gtggacgagc acacgctgct gaccattcgc aacctggagc gcatctcgac gacggacgtg
     1741 ccctacagga tccgatttcg caacctgctc ggcgaggtcg atcgcccgga gttgctcgtc
     1801 ctcgcgctcc tgcttcacga cgtcgggaaa tggcgcgacg acgatcacgc gctggagagc
     1861 gtgcgcatgg ccaccgaggc gctggagcgc ctggagctgg acgccggcgc gcgcgagacg
     1921 gtgctgttcc tgatccgcca ccacctgcgc atgtcgctcg tcgcgttccg ccgcgacacg
     1981 gaggaccccg acatcgtcaa ggagttcgcc gggttcgttg gcaccgagga tcgcctgaag
     2041 atgctgtgcc tgttgacgct cgtggacgtc gaggcggtga gtccagagac gctcacgccc
     2101 tggaaggaag agctgctctg gcggctctac gtggatacct acaaccacct cacgcagcga
     2161 tatggcgacg agctgatcga gcgcaaccag gccgggctcg acgacctgct gaagcagcgg
     2221 cccgcggatc tgtcggtccc cgagatcacg cggttcctcg agggcctgcc gcagcgctac
     2281 ctgcagctgt ttccgcgcga cgcgatctac aggcacgtgc gcctggccag cgacatccat
     2341 cccgacgagg tccacgcctc gctcgagcgc ggcgaggatg cggtctggtc gctggcggtg
     2401 gcgacgctcg acaagccgtt cctgttttcg aacatctgcg gcgtcctctc gtcgttcggc
     2461 atgaacatca tccgcgggca tgcgctcacc aacccgaacg gcctcgtcct cgacgtattc
     2521 cagttcaccg acgacgagcg ctatctcgag ctgaaccgtg acgcccagga gcaggtcgtg
     2581 cacgtgctcg aggacgtcgt gtcggcgcgc gccgacgtgg ccgagcgcct tcgtccgcgg
     2641 gagcaggggg tcctgcacgt caagggcgcg ccgcgcttcc cgcccgtggt gcgggccgac
     2701 aatcacgcgt cgggccgcta cacgatcgta gacatcgtgg cgagcaacgc gctgggactg
     2761 ctgtaccgca tcagccgcgt catctcgcgg cacggctgtg acgtcgatct cgtgctcatc
     2821 gccacggaag gcgagcgggc gatcgacgtc tttcacatca ccaaaggcgg gtcgaagctc
     2881 accgaggcgg agcagcaggc gctgacgtcc gacctctatc gcacactgga ggggtcgata
     2941 tgaagttgat caagtgcatc attcgtccga acaaggtcga cgaggtcaag gacgcgctgg
     3001 ccaagctgag catcgccggc atgaccgtga ccgaggtccg ggggcacggg aagcagaagg
     3061 ggcacacggc catctaccgc ggcaaggaat acaacgtcag cctgctgccg aagatggaga
     3121 tcgagatcgt ggtcccggac aacatggccg acgacgtcgt gaaggccgtc gtgcaggccg
     3181 cccgcacggg cgagatcggg gacggacggg tgttcgtgct gccggtgctc gagagctacc
     3241 ggatccggac cggcgagaag gaatcgtgat cctgaccacc ccaatctggc atcggcgttg
     3301 cgagcgatcg acgtggacgt tcgagtccgg ccatgtcatc gcaccgtatt ccgttcgagg
     3361 cctccgagtg ggccgagtac agccgcctcc aagctgacaa cgatcgtctg aggcacctgc
     3421 tgctcgagca gcaccgtgcg gccgatctgg ccggacacag cgtcgcgatt cgacgggtcc
     3481 gcgagttgat tgcccaggtc gcgccctcgc cgcacgccgt gtgtcttcga ggggagccgg
     3541 gtgttggaaa gagccgcgcc gcgcgcgcca ttcacgcggc gtcgtcccgg tccgccgccc
     3601 ccttcgtgac ggtggcgtgt gcggcgatgc cggacgacgt ggctgacgat cagctcttcg
     3661 gtcccggttg ggccgagacg tcgccgcgag gcccggcggc cggatgcgtc ggccgcgcgg
     3721 ccggcggcac gctgttcttc gacgaagtcg cggcgctggg gccgcttgcc cagcgcaggc
     3781 tggccagtct ggcgcgggat cgcgcggtga ccctcgccgg ccggcgggtt cccgtggacg
     3841 tgcgtctcat cgcggcgtcc agccggaaca tcgacgagct cacggcatca ggcgtgttcg
     3901 ggcgcgatct ccgcgacgag atcgccgtcg tcgcgattcc gatccccgcg ttgcgcgatc
     3961 ggaaggtcga tcttccactc ctggcggatc tcttcgtcga gctcttctca cggacgcatg
     4021 tgcgcgcggt gagaagcgtg tccgggcgcg cgatggacgt gctgatgaac tacggctggc
     4081 ctggtaatgt cacgcagctg cgtcaggcca tcgaatgggc gggcgccgcg gcggccggac
     4141 ccgtgatcca ccaccatcac ctgcccgcgg agatccaggc ggccggcgaa tcggttggac
     4201 cgttcgcgcc cgggctcggc gccgcgctcg aggcgtacga gagggagctg ctgcaggacg
     4261 cgctctcgcg cgcccgcggc gt
//
LOCUS       DBNGSH010000486         1610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3271691, whole genome shotgun sequence.
ACCESSION   DBNGSH010000486 DBNGSH010000000
VERSION     DBNGSH010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3271691"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1610)
                     /locus_tag="AC16CY_11240"
     CDS             complement(<1..>1610)
                     /locus_tag="AC16CY_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3354410.1"
                     /translation="RGGSSDPPGAIPIDEALAIARQIADALEAAHEQGIVHRDLKPTN
                     IKVRPDGAVKVLDFGLAKLTQASGPRAQASGLHLTASPTLTTPAMTQIGVIMGTAAYM
                     SPEQAKGREADKRSDIWAFGAVLFEMFTGRRAFDGEDMTDTLASVLKSDPDWTLLPAD
                     VPPALRTLMQRCLAKDRRQRVSDVSAAKFVLTELESLGLSTPLVSPRTAAAPLPGWRR
                     VLPVAATVVVTAVIVGAAAWALRPMPSPPVVVQFAFAPEGQSFTGTSAQVVAISPDGT
                     RLAYAANNRIYVRLIAELEARPVTDQSSGPSPYSVAFAPDGRSILFSMASEGGPVIRR
                     VSVDGGPVSTVTSLEGAPSSSGMSWSREGILIASAGGNGGILRVAAGGGRPERVVSIE
                     QGEIMHGPQMLPDGRTVLFTVARNTGDDRWDKAQIVARSLDGGPRRVLVEGGSDGRYV
                     KTGHLLYVVGGTVYAAPFDAGALVVTGAAVPVVSGVRRATGGQTGATQFAVSDTGTLV
                     YVPGPATMLSTARSLVIGDARGDPVPLK"
BASE COUNT          273 a          572 c          524 g          241 t
ORIGIN      
        1 ccttcagcgg gacggggtcg ccacgcgcgt cgccgatgac gagactgcgg gcggttgaga
       61 gcatcgttgc ggggccaggt acgtacacga gcgtgccggt gtcggagaca gcgaactgcg
      121 tggcgccggt ctgaccgccg gttgcgcgcc ggacgcccga tacgacgggc acagccgcac
      181 ccgtcaccac aagggcgcct gcgtcgaacg gcgccgcgta gacagtgccg ccgaccacgt
      241 acagcaggtg accggtcttc acgtaccggc catccgagcc cccttccacc aggacgcgcc
      301 gcggacctcc gtcgagcgac cgggcaacaa tctgcgcctt gtcccaccga tcgtcgcccg
      361 tattcctcgc caccgtgaag agaacggtcc gcccatcagg cagcatctgc ggcccgtgca
      421 tgatctcgcc ttgctcgata ctcacgaccc gttccggccg gccgccgccc gcggccactc
      481 gcagaatccc gccgttcccg ccagccgagg cgatgaggat gccctcgcgg ctccaactca
      541 tccccgaaga acttggcgcc ccctcgagcg aggtaaccgt cgacaccggc ccgccatcaa
      601 cagacacgcg tctgatcacc ggaccaccct cagaggccat cgagaacagg atggaccgtc
      661 cgtccggcgc gaaagcgacg gaatacggag acggtccact ggactggtcg gtcaccgggc
      721 gcgcttccag ctcagcgatc aagcggacgt agatccgatt gttggccgcg taggccagcc
      781 tcgttccgtc gggagagatg gcgacgacct gtgccgaggt tccagtgaac gactggcctt
      841 caggtgcgaa cgcgaactgg acgaccaccg gggggctcgg catcgggcgc aacgcccacg
      901 cggcggcacc gacgatgacg gcggtcacga ccaccgtcgc cgcgacggga agcacacgcc
      961 gccatcctgg aagaggagcg gcagcggtcc gcggcgatac cagcggcgtc gagagaccaa
     1021 ggctctccaa ctcggtcagc acgaacttcg ctgcggatac atccgaaaca cgctggcggc
     1081 gatccttcgc gaggcaccgc tgcatgagcg tgcgcagcgc aggcggcacg tcggcaggca
     1141 gcagcgtcca gtcaggatcg ctcttgagca ccgaggcgag cgtgtcggtc atgtcctcgc
     1201 cgtcgaaggc gcgcctgccg gtgaacatct cgaagagcac ggcgccgaac gcccagatgt
     1261 cgctgcgctt gtccgcctcg cgccccttcg cctgctccgg cgacatgtag gccgccgtgc
     1321 ccatgatcac gccgatctgt gtcatggctg gcgtcgtgag cgtgggcgac gccgtgagat
     1381 ggagccctga ggcctgagcc ctgggccctg aggcctgtgt cagcttggcc aatccgaagt
     1441 ccagcacctt caccgcgccg tccggccgta ccttgatgtt cgtcggcttc aggtcgcggt
     1501 ggacgatgcc ctgttcgtgc gccgcctcga gcgcgtcggc gatctgtctc gctatcgcca
     1561 gcgcctcgtc gatgggtatt gcgccaggcg ggtctgaaga cccgcctcta
//
LOCUS       DBNGSH010000487         3095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_555407, whole genome shotgun sequence.
ACCESSION   DBNGSH010000487 DBNGSH010000000
VERSION     DBNGSH010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_555407"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="AC16CY_11245"
     CDS             <1..880
                     /locus_tag="AC16CY_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3354411.1"
                     /translation="PSQPMGSGPIRIGGGAQGDRFVMTSATLRMLLQTAYQRASNTSL
                     SGQLQIIGGPGWMESDRYDVQARADCSGGAIPRERLQLMMQSMLEDRFQLKAHLETRE
                     LPIYNLVVAKDGPKIKLSDDQTPLPFMAAGPPRPCDSTSTAAAPPAPPPPPPGGRGFP
                     FDPGSPRPRGSVMMSSSLTGMTLQATAMPIANLVGLLQQQLGRTIVDKTDLKGLFDFK
                     LTYSPEGLSDPLFGRGGSLPPPGGAPAGPSPVPAAAEPVPSIFTAIQELGLKLESSKG
                     PVEVLVIESVQKPTEN"
     gene            complement(885..>3095)
                     /locus_tag="AC16CY_11250"
     CDS             complement(885..>3095)
                     /locus_tag="AC16CY_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3354412.1"
                     /translation="RAAAMGVAGAAAATLGSLALPADAAALAQQTAASNKIPLDLAEW
                     SYMWVNVKRAETARGAFVGGQQMYVEYMIPTQVRHPFPVVLVHGGGGQGTDWLGTPDG
                     RPGWFQYLVQEGFKVYVVDRPGHGRSPIHPELHGGFPAQALTLESIAGRFTPPNANPA
                     NVPNEYQKNHTQWPGPGNVGSDDLGRLTAGMGGSYVVQAAAPGRGAGAPGAPGRQGGA
                     AGAAPPPGRGAPPGGPPAPANQGPPGQINVQHLAWRQAGAELLDKIGPAIIMTHSAGG
                     PFGLLVAEARPNLVKATIIVEGAGGGAFAGANRWGLSTVPVTYDPPVADPSEIRTKWV
                     ANPEAGVNGYFLQEEPARKLPNLKNTKVLVATADASFASPGSPGTVAFLKQAGVQAEE
                     LRWGALGMHGNGHAMMMEKNSRQVLQPLIDWMHKSVTGSNSQPSRRASSDSLALKLAD
                     TGMFWIGTSHTKKAAYGTIHVAPMFVQYLEPAQQRFRFPVVLVHGGGGQMVHYMGLGG
                     MSGWAHHLVQAGFKVYLVDRPGHGRSVYHPDGLGEIGPLVTYDLLTRDTVTSARTPNK
                     QWPGTTGDVGDPLVDQLVAAANSAPRDNQLAQMLWRQYGSELIDRIGPSIIVTHSAGG
                     PFGWLVANERPALVKAVVSFEGATAPLVGQGGAAGTPLPNLKDMPIMYLLAERGGRDG
                     KPILDALTQSGAKAELINLKDRGILGNSHFAMFETNRRQVLDVIKGWIEQRVTS"
BASE COUNT          489 a         1063 c         1029 g          514 t
ORIGIN      
        1 gccgagccag ccgatgggca gcgggccgat acggatcgga ggcggcgcgc agggtgaccg
       61 gttcgtcatg accagcgcga ccctgcggat gctgctgcaa accgcgtatc agcgcgccag
      121 caacacgtcg ctgtcggggc aactgcagat catcggcgga ccaggctgga tggagtcgga
      181 tcgctacgac gtccaggcga gggcagactg cagcggtgga gcgattcccc gagagcgctt
      241 gcaactgatg atgcagtcga tgctggaaga tcggtttcag ctgaaggcgc acttggaaac
      301 gcgggagctc ccgatataca acctggtggt cgcgaaagat gggccgaaga tcaagctgtc
      361 cgacgatcag acgccgctgc ctttcatggc tgcaggtcca ccgcgtccgt gtgattcaac
      421 atcgacggcg gccgcccccc ctgcacctcc accgccgcct ccgggcgggc gtggcttccc
      481 cttcgacccg gggagtccgc gtccacgggg ctcggtaatg atgtcgtctt ccctcaccgg
      541 aatgacactt caggcaacgg caatgccgat tgccaatctg gtcggcttgt tgcagcaaca
      601 attaggacgc acgatcgtag acaagaccga tctcaagggc ttgttcgact tcaagctgac
      661 gtatagcccc gagggtctct cagacccgtt gttcggacga ggcggctctc tcccaccgcc
      721 tggcggggcg ccggcaggac cttcacccgt ccctgccgcg gcggagcccg tgccgtcgat
      781 cttcacggcg attcaggagc tcggactgaa gctggaatcc tccaagggcc cggttgaagt
      841 gctcgtcatc gagtccgtgc aaaagcccac ggagaactaa gcgatcacga cgtgacgcgc
      901 tgctcgatcc agcccttgat gacgtcgagc acctgacggc ggttggtctc gaacatcgcg
      961 aagtggctgt tgccgagaat gccgcgatcc ttcaggttga tgagctcggc cttcgccccc
     1021 gactgcgtca acgcatccag gatcggcttg ccgtcgcgcc cgcctcgctc ggcgaggaga
     1081 tacatgatcg gcatgtcctt caggttcggc agcggcgtcc ctgccgcgcc gccctggccg
     1141 acgagcggcg cggtcgcgcc ctcgaacgac accacggcct tgacgagagc gggacgctcg
     1201 ttggccacga gccagccgaa cggtccaccg gcggagtggg tcacgatgat cgagggtccg
     1261 atgcgatcga tgagctcgct gccgtactga cgccacagca tctgcgccag ctggttgtcg
     1321 cgcggcgccg aattggccgc ggccacgagc tgatccacca gcggatcgcc gacatcgccg
     1381 gtcgtccccg gccactgctt gttgggcgtg cgagccgagg tgaccgtgtc gcgcgtcaat
     1441 agatcgtagg tgacgagcgg gccgatctcg ccaaggccat ccgggtggta caccgatcgc
     1501 ccgtggccgg gacgatcgac cagatacacc ttgaaaccgg cctgcacgag gtgatgggcc
     1561 cagcccgaca tcccgccgag ccccatgtag tggaccatct gaccgccgcc gccgtgcacg
     1621 agcacgacag ggaaccgaaa gcgctgctgc gcaggctcga ggtactgcac gaacatcggc
     1681 gctacgtgga tcgtgccgta cgccgccttc ttggtgtgcg aggtgccgat ccagaacatg
     1741 ccggtgtcgg ccagcttgag cgccagagag tcgctcgacg cccgacgcga cggctggctg
     1801 ttgctgccgg tcacgctctt gtgcatccag tcgatgagcg gctgtagcac ttgacggctg
     1861 tttttctcca tcatcatcgc gtggccgttt ccgtgcattc cgagcgcgcc ccaccgcagt
     1921 tcctcggcct gcacgccggc ttgcttcagg aacgcgaccg tgcccggact gcccggcgat
     1981 gcgaacgagg cgtcggccgt cgccaccagc actttcgtgt tcttcaggtt cggcagcttg
     2041 cgcgcgggct cctcctgcag gaagtagccg ttgacacccg cttcgggatt ggcgacccac
     2101 ttcgtcctga tctcggaagg atcggcgacc ggcggatcgt aggtgaccgg aacggtcgag
     2161 agcccccagc ggttggcgcc ggcgaatgcg ccgccgcccg cgccctcgac gatgatggtc
     2221 gccttcacga ggttgggacg cgcctcggcg accagcaggc cgaagggccc gccggccgag
     2281 tgcgtcatga tgatcgcggg accgatcttg tccagcaact cggcgcctgc ctgcctccac
     2341 gcgaggtgct gcacgttgat ctgccctggg gggccctggt tggccggagc cggcggaccc
     2401 cctggcgggg cgccgcgacc gggcggaggc gccgcgccgg ctgctccacc ctgccgcccg
     2461 ggcgcgcccg gcgcacccgc gccgcggccg ggagcggccg cctgcacgac gtagctgccg
     2521 cccatgccgg cggtcagccg gccgagatcg tccgacccga cgttgcccgg tcccggccac
     2581 tgtgtgtggt tcttctgata ctcgttcggg acgttggcgg gattcgcgtt cggcggtgtg
     2641 aacctgccgg cgatgctctc gagcgtgagc gcctgcgccg ggaacccgcc gtgcagctcg
     2701 gggtgaatcg gcgatcgtcc gtgtccaggc cgatcgacga cgtacacctt gaagccttcc
     2761 tggacgagat actggaacca tcccggccgg ccgtccggcg tgcccagcca gtcggttccc
     2821 tgtcccccgc cgccgtgcac cagcacgacc gggaagggat gacgcacctg cgtcggaatc
     2881 atgtactcga cgtacatctg ctgcccgccg acgaacgcgc cgcgcgccgt ctcggcgcgc
     2941 ttgacgttca cccacatgta actccactcg gcgaggtcga gcgggatctt gttcgaggcc
     3001 gccgtctgct gcgccagcgc cgcggcatca gccggcagcg cgagcgatcc gagcgtcgcg
     3061 gctgcagcgc cggcgacgcc catcgcggcg gcccg
//
LOCUS       DBNGSH010000488         1823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_385890, whole genome shotgun sequence.
ACCESSION   DBNGSH010000488 DBNGSH010000000
VERSION     DBNGSH010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_385890"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1385
                     /locus_tag="AC16CY_11255"
     CDS             75..1385
                     /locus_tag="AC16CY_11255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3354413.1"
                     /translation="MPFASLKLHPALLKAVRELGFVRPTPIQADAIPPALAGRDVLAC
                     AMTGSGKTAAFLLPIVHTLIDRPRRTTRALVLTPTRELAAQILEDLNALAVHTPVTAA
                     AVYGGVGMGPQEHAFRSGVDVIIATPGRLLDHFRSAYAKLAGIEHLVLDEADRMLDMG
                     FLPDIRRVLRHIPARRQTMFFSATMPPPIGKLASEMLKNPAMINQQRQAAPAVGIAQA
                     VYPVPQDLKGPLLVQLLERNILKEALVFTRTKHRANRLADLLVQHRLNAARIHGNRSQ
                     AQRTEALAGFKSGKYRVLVATDIAARGIDVEALSHVLNFDVPAVPEDYIHRVGRTGRA
                     ELTGDAFTFVAPDEEGDLRAIERAIGKTLPRVTLPDFDYRARPHTRLEIPLGERIAAI
                     RRRKAEERARGKAKAQRPAAHGHGHVAVPARMSRPRRGPRRRPF"
     gene            1567..>1823
                     /locus_tag="AC16CY_11260"
     CDS             1567..>1823
                     /locus_tag="AC16CY_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354414.1"
                     /translation="MLPSSRTLDGFARESRAHDGLDITTLDAGVTLLVRTRHSSYRLR
                     VLDGARRLVLAEGGIFLEPTVVHLSGATFGGSTLKLGWILVG"
BASE COUNT          305 a          629 c          611 g          278 t
ORIGIN      
        1 gtcggaacag tcatcgaagg gagtgtactc caccggaccg gggacgatcc gttgacaacg
       61 actaagatgg cttcatgccc ttcgcgtcac tcaagctcca cccggctctt ctgaaggccg
      121 ttcgtgaact cggcttcgtg cggcccacgc cgatccaggc cgatgcgatt cctccggcgc
      181 tcgccgggcg cgacgtgctc gcctgcgcca tgacggggag cgggaagaca gccgcgttcc
      241 tcctgccgat cgtccacacc ctcatcgatc gcccgcgacg gacgacgcgc gcgctcgtgt
      301 tgacgccgac gcgcgagctg gccgcgcaga ttctggagga cctgaacgcg ctcgcggtcc
      361 atacgccggt gacggcggcg gccgtgtacg gcggggtcgg catgggaccg caggagcatg
      421 cgttccgcag cggcgtggac gtcatcatcg ccacacccgg ccggctgctc gatcacttcc
      481 gctcggcgta tgcgaagctg gccggcatcg agcacctcgt tctggacgaa gcagatcgca
      541 tgctcgacat gggcttcctg cccgacatcc gccgcgtgct ccgccacatt ccggcgcgcc
      601 gccagacgat gttcttcagc gcgacgatgc cgccgcccat cgggaagctg gcgtcggaga
      661 tgctgaagaa tcccgcgatg atcaatcagc agcgacaggc cgcgcccgcc gtcggcatcg
      721 cgcaggcggt gtacccggtg ccgcaggacc tgaagggccc gcttctcgtc cagctcctgg
      781 agcggaacat cctgaaggaa gcgctggtgt tcacgcgcac caagcatcgc gcgaaccggc
      841 tggcggacct gctggtgcag catcgcctca acgcggcgcg catccatggc aaccggtcgc
      901 aggcgcagcg gaccgaagcg ctcgcgggct tcaagagcgg gaagtaccgc gtgctggtgg
      961 ccaccgatat cgcggcccgc ggcatcgacg tcgaggcgct cagccacgtc ctcaatttcg
     1021 acgtgcccgc ggtgcccgag gattacatcc atcgtgtggg ccgcaccgga cgcgccgagc
     1081 tcacgggcga tgcattcaca ttcgtggccc cggacgagga gggcgacctg agggcgatcg
     1141 agcgcgcgat cggaaagacc ctgccgcgcg tcacgcttcc cgatttcgac taccgcgcca
     1201 ggccgcacac gcggctcgag attccgctgg gcgagcgcat cgcggcgata cggaggcgga
     1261 aggccgagga acgtgcgcgc gggaaggcga aagcgcagcg gccggcggca cacggacacg
     1321 gccacgttgc cgtgcccgcg cgcatgtcgc gtccccgccg cggcccgaga cgccgtccgt
     1381 tttgacgaca gcagcgccct gacccgaatg gctcaggtgg ggggactctc ccagtggtgc
     1441 gggaaactcc ccacgccaaa cctggtgcgc acggaattgt ttgagaaata cgagcatacc
     1501 gacgccgatc tggctctcgt cggctggcag cggaattgcc ccactcgcgc ccaggtggag
     1561 gacgcgatgt taccaagtag ccgcacgctg gatggcttcg cacgcgagtc gagggcacat
     1621 gacgggctgg acatcacgac gctcgacgcc ggggtcacac tccttgtgag gacccggcac
     1681 tcatcctacc gactccgcgt ccttgacggc gcccggcgtc tggtgctggc tgagggcggg
     1741 atctttctcg agccaaccgt ggtgcacctc tcgggcgcca cgttcggcgg cagcacgctg
     1801 aagctcggct ggatactcgt cgg
//
LOCUS       DBNGSH010000489         1523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_215912, whole genome shotgun sequence.
ACCESSION   DBNGSH010000489 DBNGSH010000000
VERSION     DBNGSH010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_215912"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="AC16CY_11265"
     CDS             <1..1215
                     /locus_tag="AC16CY_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR3354415.1"
                     /translation="TFKEGEWMSIDGTAGKVYAGQVKTASSEIVAGLIDGDASARKNE
                     KFRSFQQLMKWCDKATRMNVRTNADTPEQARQAIAFGAVGIGLCRTEHMFFEGDRIDA
                     MREMILAETTEARKAALAKILPFQRDDFAGIFRALDGYPASMRFLDRPLHEFLPNTPE
                     QQKDLAESMGVAADAIEARVNQLHEANPMLGFRGCRLGIGYPEISAMQARAVFEAAAI
                     VQKEGIKVRPEIMIPLVAFKKELDLQIEVVHQVAREVQAERKVKLNYLVGTMIEIPRG
                     ALTADEIAQSADFFSFGTNDLTQTGLGMSRDDSGSFLPKYSELEIVKKNPFATIDQTG
                     VGQLVDIAVTKGRKTKPGLKLGICGEHGGDPASIHFFERVGLDYVSCSPYRVPVARLA
                     AAQAALAAKVEE"
     gene            1245..>1523
                     /locus_tag="AC16CY_11270"
     CDS             1245..>1523
                     /locus_tag="AC16CY_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HIR3354416.1"
                     /translation="MVSVYVHEGGETKLTQTVEREWLEATSGVTFWLDLSAPGDDEFR
                     LLSDLFKFHPLSIDDARSALQFPKVEPYPEYLYLVLHGIDAEESTRRFS"
BASE COUNT          290 a          470 c          511 g          252 t
ORIGIN      
        1 acgttcaagg agggcgagtg gatgtcgatc gatgggaccg cgggaaaggt ctacgcgggc
       61 caggtcaaga cggcgtcgtc ggagatcgtt gccggcctca tcgacggaga cgcgtcggcc
      121 cggaagaacg agaagttcag gagtttccag cagctcatga agtggtgtga caaggcgacg
      181 cggatgaacg tccgcaccaa cgccgatacg cccgagcagg cgcggcaggc gatcgcgttc
      241 ggcgcggtcg gcatcggcct gtgccgcacc gagcacatgt tcttcgaggg ggatcgcatc
      301 gacgcgatgc gcgagatgat cctcgccgag acgaccgagg cgcggaaggc ggcactggcg
      361 aagatccttc cgttccagcg ggacgacttt gccggcatct tccgcgcgct cgacggctat
      421 ccggcgtcca tgcgcttcct cgatcggccg ctgcacgagt tcctgcccaa tacgcccgag
      481 cagcagaaag atctcgcgga gagcatgggg gtcgccgctg acgccatcga ggcacgcgtc
      541 aaccagctcc acgaggccaa tccgatgctc ggcttccggg gctgccgtct cggcatcgga
      601 taccccgaga tttcggcgat gcaggcccgc gccgtcttcg aggccgcggc gatagtccag
      661 aaggaaggga ttaaagtccg gcccgagatc atgattccgc tcgtggcgtt caagaaggag
      721 ctcgacctgc agatcgaagt ggtgcaccag gtggcgcgcg aggtgcaggc cgaacggaag
      781 gtcaagctga attacctggt cggcacgatg atcgaaattc cgcgcggggc gttgactgcc
      841 gatgagatcg cccagtcggc agacttcttc agcttcggca ccaacgacct cacccagacc
      901 gggctcggga tgtcgcgcga cgattccggc tcgttcctcc cgaagtactc ggagctcgag
      961 atcgtgaaga agaacccgtt tgcgacgatc gaccagacgg gagtcggcca gctggtggac
     1021 atcgcggtga cgaaagggcg caagacgaag cccggactga agctcggcat ctgcggcgag
     1081 cacggcggcg atccggcgtc gattcacttc ttcgagagag tcggcctcga ctacgtcagc
     1141 tgctcgccct atcgcgtgcc cgtggcccgg ctggcggccg cgcaggcggc cctggctgcg
     1201 aaagtggagg agtgaggacg ccctcctcac cgcggcatta acgcatggtc tccgtctacg
     1261 ttcacgaggg cggcgagacg aagctgacac aaacggtgga acgggagtgg ctcgaggcaa
     1321 cctcgggcgt cacgttctgg ctcgacctgt cggcgcccgg cgacgacgag ttccgtctgc
     1381 tgagtgatct cttcaagttc caccctctct cgattgacga cgcgcggagc gcgctgcagt
     1441 ttccgaaagt cgagccatat cctgagtacc tgtacctcgt gctgcacgga atcgacgccg
     1501 aggagagcac ccggcgcttc tct
//
LOCUS       DBNGSH010000490         5002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2253890, whole genome shotgun sequence.
ACCESSION   DBNGSH010000490 DBNGSH010000000
VERSION     DBNGSH010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2253890"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC16CY_11275"
     CDS             complement(<1..190)
                     /locus_tag="AC16CY_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIR3354417.1"
                     /translation="MILRYNELAQYRKKVAMVDGAFDPLHHGHVEYFSRARQVGTPLL
                     CNIAPDFYTSAKHPPLLPA"
     gene            complement(222..2432)
                     /locus_tag="AC16CY_11280"
     CDS             complement(222..2432)
                     /locus_tag="AC16CY_11280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3354418.1"
                     /translation="MPRVIIGSPLYNHANDFREAIESILGQTYADFALVLVDDCSTDA
                     TPEIAREYAGLDPRVTYLANPQRLGLVDNARRAFAVACERHPEAEYFAWASDHDLWHP
                     RWLQALVEALDRHPEVVLAYPLNRRIGPSGELLARKPWSFDTLGITDSWKRLNLSIVK
                     MSAGNMVYGLYRIPLLARAGVYRRVLVPDRLLMTELAVYGQFKQVPEVLWFRRWYGRI
                     FSLGRQRKTFFPEGRPLYMYAPWWVSHGVSLFWTFGVQKRGMPAVTGAAGAILGLKYL
                     VFSGLFHAWQTLRAWRIGLLERLGAIRPYERRLRLVTREVVRRGVVDWTSSHTKHYVG
                     AKARRRAMSRVKKGVKRAAFQSVRRPGLALLRRLREIPLVRSRVVPSLLRQELDQIPA
                     APIVAALERELDRVGKSSAPILVGPWVSEVGFELLYWIPFLTWAVKAFGLQDRRMVVV
                     SRGGARLWYQHLTTEYVDVFDLMTVEEYRAYSEERWSDKGHQKQFDVTDVERRILGLT
                     RTRLGLGETQLLHPSLMYKLLRYYWYEKAPAGLLTKHTEYRRLPAVERGDALKDLPSD
                     YVAVRFYFRPSFPDTPENRRFAAGVIRALSRDVPVVLLNTGLDLDDHEDLQVPGGMGV
                     HTVERLMTPQGNLEMQTRIVANARAFVGTYGGLAYLGPLYGVPSVGFYSHESELVPAH
                     LDVGWRLGKVTGAPLSTIDVKGAGMLRLLFGAGELDWAGGSRQRTAGGSAVGRL"
     gene            complement(2466..3500)
                     /locus_tag="AC16CY_11285"
     CDS             complement(2466..3500)
                     /locus_tag="AC16CY_11285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar O-methyltransferase"
                     /protein_id="HIR3354419.1"
                     /translation="MNTATAAPTFRERLEALRASPAFRNYLMVRERVVEMLAQVASMP
                     DYRPSAYWEEELANFEYMLDASPFVIDKLRHHTFHVTGLRVHDYRTHRDKARTKLAEK
                     LHALQQVAPPGLLVPEWRELGGFGFDIDGQLYNVDTLKFFEVLIALHKGSVLQEFLDN
                     GERRLVWEIGAGWGGFPYQFKSLCPNVTYVISDFPELFLFSATYLMTAFPGAKVAFWG
                     EQPIERLFERWREYDFILLPNTALADLKPERLDLTINMVSFQEMTAGQVTAYVERAHA
                     LGCPFLYSLNREKSPYNPEIESVSEIISRTYWPREVSVLSVSYQKMIGDRPTPTDYKH
                     IIGWRRIAKQ"
     gene            complement(3529..>5002)
                     /locus_tag="AC16CY_11290"
     CDS             complement(3529..>5002)
                     /locus_tag="AC16CY_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354420.1"
                     /translation="AEGAEALEGLAGSVEVLGQTPVREGPWATVAGAVRGTIDYVRYL
                     HPTFADAPYLRDRMRKALPPVFSFFARWDTASRGTTRALVRVLTTMERAIPSSRTIET
                     FIDSRRPDAVLVTPLVTDRCTQVDLVKGAQALGIPAALCVASWDHLTTKGLIRVEPDL
                     VSVWNEEQKKEAVEFHGTPAGSIAVTGAQPFDRWFERVPTDRAAFCRKVGLAAERPYV
                     LFAGSTASISSPDAELIFVRRWIEALRREPGLAGVGVLVRPHPYNSTHWSDVDFADLH
                     GVVVYPRAANPVNEADRQDYFDSLFHSEAVVGVNTTAMVEAAIVGRTVHTVLADEFKD
                     TQRGTLHFRYLLSENGGFLRVAPDLAAHARQLAETLRTPEIGREACARFVATFIRPHG
                     IGVASTPILVDALVRLASGRRSARRVPWRLYPLSAFLWSIGVADLMRHPDLFRRTVRK
                     QFKIVRKRYRNAQKRRRRRAVASVHVSDETRPEIPRRKVG"
BASE COUNT          873 a         1667 c         1669 g          793 t
ORIGIN      
        1 gggccggaag cagcggcggg tgcttcgcgc tcgtgtagaa atcgggcgcg atgttgcaga
       61 gcagcggcgt gccgacctgc cgcgccctgc tgaaatactc gacgtgcccg tgatggagcg
      121 gatcgaaagc gccatcgacc atggccacct tcttccgata ctgcgccagt tcgttgtatc
      181 tgaggatcat ccggtctgtt ttcggcctac aagtctacgg tctacagtct gcctactgcg
      241 ctgccgcctg ctgtccgctg cctgctcccc ccggcccagt ctagctcgcc agcgccgaag
      301 agcagccgca gcattccggc ccccttcacg tcgatcgtcg agagcggagc gccggtcacc
      361 ttgcccagcc gccatccgac gtcgagatgc gcgggcacga gctccgattc gtgcgaatag
      421 aagccgaccg acggcacgcc gtacagcggc ccgaggtagg cgagcccccc gtaggtgccg
      481 acaaacgcgc gcgcgttcgc aacgatgcgg gtctgcatct ccaggttgcc ctgcggagtc
      541 atcagccgtt caaccgtgtg cacacccatg ccgccgggaa cctgcagatc ctcgtgatcg
      601 tccaggtcga gccccgtgtt gaggagcacc accgggacat cgcggctgag cgcgcggatc
      661 acgccggcgg caaagcgccg gttctcgggc gtgtcgggga acgacgggcg gaaatagaac
      721 cggacggcca cgtagtcgct cggcagatcc ttcaacgcgt cgcctcgctc gaccgccggc
      781 agccggcggt attcggtgtg cttggtgagg aggccggcgg gcgccttctc gtaccagtag
      841 tagcgcagga gcttgtacat cagcgacggg tgcagcagtt gggtctcgcc gaggccgagc
      901 ctggtgcggg taagaccgag gatcctgcgt tcgacgtcgg tgacgtcgaa ctgcttctgg
      961 tggcccttgt ccgaccagcg ctcctcgctg taggcgcgat actcctcgac ggtcatcagg
     1021 tcgaacacgt ccacgtactc ggtcgtcagg tgctgatacc acaggcgggc gccgccgcgc
     1081 gacacgacga ccatccggcg gtcctggagg ccgaaggcct tcaccgccca ggtgaggaac
     1141 ggaatccagt acaacagctc gaagccgacc tcggagaccc agggcccgac gaggatcggc
     1201 gcggacgact tgccgactcg atcgagctcc cgttcgagcg ccgcgacgat cggggcggca
     1261 ggaatctgat ccagctcctg cctgaggagc gaggggacga cgcggctgcg cacgaggggg
     1321 atttcgcgca ggcgccggag cagcgccagg ccgggacgcc ggacggactg gaacgccgcg
     1381 cgcttcacgc ctttcttgac gcgcgacatg gcgcgcctcc gtgccttcgc gccgacgtag
     1441 tgcttcgtgt gactcgacgt ccagtcaacg accccgcgcc tgacgacctc gcgcgtcacg
     1501 agccgcaggc gacgctcata cggcctgatg gccccgagcc gttcgagcag gccaatccgc
     1561 cacgcgcgca gcgtctgcca ggcatggaac aggccgctga acaccaggta cttcagcccg
     1621 aggatcgcgc cggcggctcc cgtgacggcc ggcatgcccc gcttctgcac gccgaaggtc
     1681 cagaagagac tcacgccgtg gctgacccac cagggcgcat acatgtacaa cggccggcct
     1741 tcggggaaga acgtcttccg ctggcggccc aggctgaaga tccggccgta ccagcgccgg
     1801 aaccacaaga cttccgggac ctgcttgaac tggccgtaca ccgccaactc ggtcatcagc
     1861 agccgatccg gcaccagcac acggcgatac acgcccgccc gggcgaggag cgggatgcga
     1921 tacaagccgt acaccatgtt ccccgccgac atcttgacga tgctgagatt gagccgcttc
     1981 caggaatcgg tgatgccgag ggtatcgaag ctccagggct ttctggccag cagctcgccg
     2041 gaggggccga tgcgccggtt caagggatac gcgagaacga cctcgggatg tcgatcgagc
     2101 gcctccacca gcgcctgcag ccagcgtgga tgccacaggt cgtgatcgct cgcccaggcg
     2161 aaatactcgg cctcgggatg gcgctcgcac gcgacggcaa aggcgcggcg cgcgttgtcc
     2221 acgagcccga gccgctgcgg gttggcgaga tacgtgacgc gagggtcgag ccccgcgtac
     2281 tcgcgcgcga tctcgggagt ggcatcggtc gagcaatcgt cgacgagaac cagcgcgaag
     2341 tcggcgtagg tctgaccaag gatcgattcg atcgcctcac ggaagtcgtt ggcgtggttg
     2401 taaaggggcg acccgatgat gacgcggggc atactgcctg ctgcgcgcgg gctcaagccc
     2461 gcgctctact gctttgcaat ccgccgccag ccgatgatgt gcttgtagtc ggtcggcgtc
     2521 ggccggtcgc cgatcatctt ctgataggac acgctcaaga cggagacctc gcgcggccag
     2581 taggtacgcg agatgatctc gctgacgctt tcaatctcgg ggttgtaggg cgacttttcc
     2641 cggttcaggc tgtagaggaa cgggcagccc agcgcgtgag cgcgctcgac gtaggccgtc
     2701 acctgccccg ccgtcatttc ctgaaacgag accatgttga tcgtcaggtc gaggcgctcc
     2761 ggcttcaggt cggcaagcgc cgtgttgggg agaaggatga agtcgtactc ccgccatcgc
     2821 tcgaacagcc gctcgatcgg ctgctcgccc cagaacgcca cctttgcgcc ggggaacgcc
     2881 gtcatcaggt aggtcgccga gaacaggaac agctcgggaa aatcgctgat gacgtaggtc
     2941 acattcgggc acagcgactt gaactgatag ggaaacccgc cccacccggc gccgatctcc
     3001 cagacgaggc ggcgttcgcc gttgtccagg aactcctgca gcaccgagcc cttgtggagc
     3061 gcgatgagca cctcgaagaa cttgagcgtg tcgacgttgt acagctggcc gtcgatgtcg
     3121 aagccgaagc cgccaagctc gcgccattcc ggaacgagca gaccgggtgg agccacctgc
     3181 tgcagggcgt gcagcttctc ggcgagcttg gtgcgggcct tgtcgcgatg cgtccggtag
     3241 tcgtggacgc ggaggccggt gacgtgaaag gtgtggtgcc ggagcttgtc gatgacaaac
     3301 ggagacgcgt cgagcatgta ctcgaagttc gcgagctcct cttcccagta cgcgctcgga
     3361 cggtaatccg gcatcgaggc gacctgcgcc agcatctcca cgacccgctc gcgcaccatc
     3421 aggtagttcc tgaaggccgg cgatgcgcgg agcgcctcga gccgctcgcg gaaagtcggc
     3481 gcggcggttg ctgtattcac gactggccta tccaccatcc actatccact aaccaacttt
     3541 gcggcggggg atctccggac gcgtctcgtc cgacacgtgc actgacgcga ccgccctgcg
     3601 cctccgccgc ttctgcgcgt tccggtaccg cttgcggacg atcttgaact gcttccgaac
     3661 cgtcctgcgg aacagatccg gatgacgcat caggtcggcg acgccaatcg accacaggaa
     3721 cgcgctgagc gggtacagcc gccacggcac gcgccgggca gaccgcctgc cggaggcgag
     3781 ccggacgagc gcgtccacga gaatgggagt ggaggcgacg ccgatgccat gcggacggat
     3841 gaacgtggcg acgaaccgcg cgcacgcctc gcgcccgatc tcgggtgtcc tcagcgtctc
     3901 ggcgagctgc cgcgcgtgcg cggcgaggtc cggcgccacg cggaggaacc cgccgttttc
     3961 cgacaggagg tacctgaagt ggagcgtccc gcgctgcgtg tccttgaact cgtcggccag
     4021 cacggtgtgg accgttcgtc ccacgatggc ggcttcgacc atcgccgtcg tgttgacgcc
     4081 gacgacggcc tcgctgtgaa agagggagtc gaaataatcc tggcgatccg cctcgttgac
     4141 cgggtttgcc gctcgggggt acacgacgac gccgtgtaga tccgcgaagt ccacgtcgct
     4201 ccagtgcgtg gagttgtacg gatgagggcg gacgagaacg cccacccccg ccagcccggg
     4261 ctcccgccga agggcctcga tccagcggcg cacgaatatc agctcggcat caggcgatga
     4321 gatcgacgcg gtcgatccgg caaagagcac gtacggacgc tcggccgcca gcccgacctt
     4381 gcggcagaaa gccgcccggt cggtggggac gcgctcgaac cagcgatcga agggctgcgc
     4441 gccggtgacg gcgatggagc ccgccggcgt gccgtgaaac tcgacggcct ctttcttctg
     4501 ctcctcgttc cagaccgaga cgaggtccgg ttccacgcgg atcaggcctt tggtcgtcag
     4561 gtgatcccag ctcgctacgc agagcgcggc cggaatcccg agcgcctgcg cacccttgac
     4621 cagatccacc tgcgtgcacc ggtcggtgac gagcggcgtc accagcaccg catcggggcg
     4681 gcgggaatcg atgaaggttt cgatcgtccg gctgctcgga atcgcgcgct ccatcgtcgt
     4741 cagcacgcga acgagggcgc gggtcgttcc gcggctcgcc gtgtcccacc ttgcgaaaaa
     4801 cgagaacacc ggtggaagcg ccttccgcat tcgatcgcgg aggtagggcg cgtcggcaaa
     4861 ggtcgggtgc agatagcgga cgtagtcgat cgtgccgcgc acggcgcctg ccacggtggc
     4921 ccacgggcct tcgcgcacgg gagtctgccc gaggacttcg accgagcccg ccaggccctc
     4981 gagcgcctcg gcgccctccg ct
//
LOCUS       DBNGSH010000491         3532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2253984, whole genome shotgun sequence.
ACCESSION   DBNGSH010000491 DBNGSH010000000
VERSION     DBNGSH010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2253984"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="AC16CY_11295"
     CDS             complement(<1..1325)
                     /locus_tag="AC16CY_11295"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR3354421.1"
                     /translation="MNTKKLPSRITVEGLDRAPHRAFLRALGLTEIDLQKPFVGVAST
                     DGRVTPCNALLGEFAREACAAVREAGGVAFDFASASVADSMSMNHGGMRYSLVSREII
                     ADGVEAVVRGHAYDALVGFAGCDKTLPGMMMAMVRLNLPSVFMYGGAALPGTWRGRDV
                     TVLDTYEAVGAVLAGDMAEKELNELERVCLPTLGSCPGQFTANTMAMVSETLGLALAG
                     SATLPAVDSYRRQFSRRAGETVMRILRDGGPLPRELVTKKSLENACAAVAATGGSTNA
                     ALHLPAIAHEAGIRFTLDDFARVAKRTPLIADMKPGGRFLAKDLHAVGGVEAVLKELL
                     KRGALHGSCLTLSGSSLEQELAEHPGADGSVVRKEPIMKSGGLVVLKGNLAPDGALIK
                     VAGLKSLVFEGPARVFDSEEACAEVVKQRRYKQGEVLVIRYEGPKGGPG"
     gene            complement(1288..2184)
                     /locus_tag="AC16CY_11300"
     CDS             complement(1288..2184)
                     /locus_tag="AC16CY_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3354422.1"
                     /translation="MRILVTGAAGYIGSALVKALDGHDVLATDQSAPAPIIGNLAYPQ
                     FARSLITRDVQAVFHLASLVSGGAEQSFELGTKVNLDATRDLLEACRRAGHRPKFIFA
                     SSIAVYGGVSEVIDETPVAPRLSYGAQKLVCEILIDDYTRRGYIDGRALRLPAVLVRP
                     GGPNTAVSGWSSAVMREPLAGRDYVCPVAPETRLACISLRKVVQSFLHALALPFERIG
                     PRRTMLLNGISASAQEMWDAVKFKAAGNVRFSPDPTVQSVMDGAPKSTFSKRSAELGF
                     PASASIQEIVREYEEATLAHHG"
     gene            2213..3169
                     /locus_tag="AC16CY_11305"
     CDS             2213..3169
                     /locus_tag="AC16CY_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3354423.1"
                     /translation="MVRALVALVLASFSVASWSQAYPSRPVKIIVPYGVGGSADVYAR
                     FLGAKLQESLGQSVVVENRPGGGSIVGTDVVAKSDPDGYTLLMMSNTHTVNETLIPKK
                     PFDLMRDFAPITGVSYSDLLMVVHPSVPASSVKEFVALAKSKPGALNYASSGNGTPYH
                     MAGELFKAMAGVDIVHVPHKGSDQARAAVMGGQVQMMFDAVPTMAAQAKAGKVKALGT
                     TGMKRSSVTPDLPTLDEAGIKGYESGLWLGLMAPAKTPRPVLEKLNVEVNKLLDSANV
                     KQTMAKQGTTTMGMSIEQFDKFLRDEIVKWAKIVKTSGAKVD"
     gene            3166..>3532
                     /locus_tag="AC16CY_11310"
     CDS             3166..>3532
                     /locus_tag="AC16CY_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3354424.1"
                     /translation="MTPDQLAIVEVVHNWALWRDAGDWERFATVWHDDGWMTATWFQG
                     PAREFIEVSKAGFEKGVSILHFLGGSSVDLKGSRAICQTKMTISQRGSCDGEVVDVVC
                     TGRFYDFFEKRAGKWAIVRR"
BASE COUNT          628 a         1155 c         1172 g          577 t
ORIGIN      
        1 atgcccggcc cgcccttggg tccttcgtag cggatgacga gcacttcccc ctgtttgtat
       61 ctgcgttgct tgacgacttc ggcgcacgcc tcttcggagt cgaacacgcg cgccggcccc
      121 tcgaacacga gcgacttgag gcccgcgacc ttgatcaacg caccgtcggg cgccaggttg
      181 cccttaagga ccacgaggcc gccgcttttc atgatcggtt ccttgcggac cacgctcccg
      241 tcggcaccgg gatgttctgc gagctcttgt tccagtgaac tgccggagag cgtcaggcaa
      301 gagccgtgca acgcgccccg tttcagcagt tctttcaaga ctgcctcgac gccccccacc
      361 gcatgcaggt ccttcgcaag gaagcggccg ccgggcttca tgtcggcgat gagcggcgtg
      421 cgctttgcga cgcgcgcgaa atcgtcgagc gtgaaacgga tgccggcttc gtgtgcgatc
      481 gcgggaagat gcagcgcggc gttggtcgag ccgccggtgg ccgcgaccgc ggcgcaggcg
      541 ttttcaagac tcttcttcgt gaccagctca cgcggcagcg gaccgccgtc cctgaggatg
      601 cgcatgacgg tctcgcccgc tctccgggaa aattgcctgc gataagaatc gaccgcgggc
      661 aaggtcgcgg agccggcgag cgcgagaccg agagtctcgg acaccattgc catggtattc
      721 gcggtgaact gtccggggca ggaaccgagc gtcggcaggc aaacccgctc gagctcgttc
      781 agctcctttt cggccatgtc gccggcaagg acggccccca cggcttcgta agtgtcgagc
      841 accgtgacgt cccggccgcg ccaggtgccg ggaagcgcgg cgccgccgta catgaacacc
      901 gagggcaggt ttagccgcac catcgccatc atcatgccgg gcagggtctt gtcgcagccg
      961 gcgaaaccca cgagcgcgtc gtacgcatgg ccgcgcacga ccgcctccac gccatcggcg
     1021 atgatctctc gcgatacgag cgagtagcgc atcccgccgt ggttcatcga catcgaatcc
     1081 gcgaccgacg cggaggcgaa gtcgaacgcc acgccgcccg cctcgcgcac cgcggcgcac
     1141 gcttcgcgcg cgaattcccc gagcagcgcg ttgcacggcg tcacgcggcc gtccgtggag
     1201 gcgacgccga cgaacggctt ttgcagatcg atctccgtta atcccaacgc gcgcagaaac
     1261 gcacggtgcg gcgcccggtc gagaccctca accgtgatgc gcgagggtag cttcttcgta
     1321 ttcacgcacg atttcctgga tgctcgcgct cgcgggaaag cccaattccg cgcttctttt
     1381 cgagaacgtc gacttgggcg cgccgtccat gaccgactgg accgtcggat ccgggctgaa
     1441 gcgcacgttg ccggccgcct tgaacttgac tgcgtcccac atctcctggg cgctcgcgct
     1501 gatgccattc agcagcatgg tgcgccgcgg gccgatcctc tcgaacggga gggcgagcgc
     1561 gtgcaggaac gactgcacga ccttcctgag cgagatgcag gcgagccgcg tctcgggggc
     1621 gaccgggcac acataatcgc ggccagcgag cggctcgcgc atcaccgcgc tcgaccaccc
     1681 cgacaccgcg gtgttcgggc cgccgggacg caccagcacc gccggcagac gcagagcgcg
     1741 tccgtcgatg tagccgcggc gggtgtagtc gtcgatcaga atctcgcaca cgagcttttg
     1801 cgcgccgtac gagagacgcg gcgctacagg ggtttcatca attacttcac taaccccacc
     1861 ataaaccgcg atcgacgagg cgaaaatgaa cttcggccgg tggccggctc gccggcaggc
     1921 ttcgagaagg tcgcgcgtcg cgtccaggtt caccttggtc ccgagctcga agctctgctc
     1981 ggccccaccc gagaccagcg aggcgagatg gaatacggcc tgcacatcgc gcgttatcag
     2041 ggaccgggcg aactgcgggt aggccaggtt gccgatgatc ggcgccggcg ccgactggtc
     2101 ggtggcgaga acgtcgtgcc cgtcgagcgc cttcaccagt gcggaaccga tatagcccgc
     2161 cgcgccggtg acgagtattc tcatgggaac aagcattcta aaaggaggag gtatggttcg
     2221 cgcgcttgtc gcactcgttc tagcgtcgtt cagcgtcgcc tcgtggtcgc aggcgtatcc
     2281 gtcgcggccg gtgaagatca tcgtgcccta cggggtcggc ggctcggccg acgtctacgc
     2341 gcgcttcctc ggcgcgaagc tgcaggaaag cctcggccag agcgtcgtgg tcgagaaccg
     2401 cccggggggt ggctccatcg tcggcaccga cgtcgtggcc aagtccgatc ccgacggcta
     2461 tacgctgctc atgatgtcga acacgcatac cgtgaacgaa acgctaatcc cgaagaagcc
     2521 gttcgacctg atgcgcgact tcgcgcccat caccggcgtg agctactccg atctcctcat
     2581 ggtggtgcat ccctcggtgc ccgcgagttc ggtgaaggag ttcgtcgccc tcgcgaagtc
     2641 gaagcccggc gcacttaact acgcgtcctc gggcaacggc acgccctacc acatggcggg
     2701 cgagctgttc aaagccatgg ccggcgtgga catcgttcac gtgccacata agggcagcga
     2761 tcaggcgcgc gccgcggtga tgggcggcca ggtgcagatg atgttcgacg cggtaccgac
     2821 gatggcggcg caggcgaaag ccggcaaggt gaaggcgctc ggcaccacgg gcatgaagcg
     2881 ctcgtcggtg acgcccgacc tgccgacgct cgacgaagcc ggcataaagg gctacgagtc
     2941 gggcctctgg ctcggcctga tggcgccggc gaagacgccg cggccggtgc tcgagaaact
     3001 gaacgtcgaa gtgaacaagc tcctcgactc ggccaacgtc aagcagacca tggccaagca
     3061 gggcaccacg accatgggca tgtcgatcga gcagttcgac aagttcctgc gcgacgagat
     3121 cgtgaagtgg gcgaagatcg tgaagacctc cggagcgaag gtcgattgac gccggatcag
     3181 cttgcgatcg tcgaagtggt ccacaactgg gcgctgtggc gcgacgccgg cgactgggag
     3241 cgcttcgcca ccgtctggca cgacgacggc tggatgaccg ccacctggtt ccagggcccg
     3301 gcgcgcgagt tcatcgaggt gagcaaggcg gggttcgaga aaggcgtgag catcctgcac
     3361 ttcctcggcg gctcgagcgt ggacctgaag ggtagccgtg cgatctgcca gacgaaaatg
     3421 acgatcagcc agcgcgggag ctgcgacggt gaggtggtcg acgtggtgtg caccggccgg
     3481 ttctacgatt tcttcgagaa gcgcgccggc aagtgggcga ttgtgcggcg cc
//
LOCUS       DBNGSH010000492         1961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_725908, whole genome shotgun sequence.
ACCESSION   DBNGSH010000492 DBNGSH010000000
VERSION     DBNGSH010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_725908"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="AC16CY_11315"
     CDS             complement(<1..962)
                     /locus_tag="AC16CY_11315"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3354425.1"
                     /translation="MAPEHVRIFDTTLRDGEQAPGFSLRPSEKLQLARQLDALGVDII
                     EAGFPIASPADAEAVRQISTEIRRPVIACLARCHKADLEKAAWAIKPAEQGRIHTFIA
                     TSDLHLKAKLRMSREQCLEVAVDSVRYARQHTSDVQFSAEDATRSDFDFLCKVVESVI
                     AVGATTINLPDTVGITTPDETLDFFTRIRSRVPNADGVVFSAHCHDDLGLAVANSLSA
                     IQGGARQVECTVNGVGERAGNAALEEIVMALRVRADRMPFTTRVNTKEIYPSSDMLTK
                     LTGQGVQVNKAIVGRNAFAHEAGIHQDGMLKDRRTYEIMRAEDVG"
     gene            1147..>1961
                     /locus_tag="AC16CY_11320"
     CDS             1147..>1961
                     /locus_tag="AC16CY_11320"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3354426.1"
                     /translation="MKLHRTQPAVSQSVRRLEDSVGERLFDRATKDASLTEAGRLLRD
                     YAERLLRLSEEAETAVKDLRDLRRGRVLVGANEASVHAVLPLIARFRDGHPLVHVDVR
                     RIPARQLGAEVAQGSLDFGVLTFQPAEPGLSSIVLGNDELVMLVHPSHELASRRDVML
                     SECARQTVIAHNDPSHVRERVLRLFEQHHIPANILVSLPSLEGIKRAVAMQMGVALLP
                     RRCAESEIERGELVALAMPEVRIKRQVRLIFRKSGERSHASAAFLAMLEEKKKP"
BASE COUNT          314 a          659 c          659 g          329 t
ORIGIN      
        1 cccacgtcct cggcgcgcat gatctcgtag gtgcggcggt ccttgagcat cccgtcctgg
       61 tgaatccccg cctcgtgagc gaacgcgttc ctccccacga tcgccttgtt cacctgcacg
      121 ccctggccgg tgagcttggt gagcatgtcg ctcgacggat agatctcttt cgtgttgacg
      181 cgggtcgtga acggcatgcg atcggcgcgg acgcgcagcg ccatgacgat ctcctcgagc
      241 gcggcgttgc cggcgcgctc gccgacgccg ttcaccgtgc actcgacctg gcgcgcgccg
      301 ccctggatgg ccgacagcga attggcgacg gcaaggccga ggtcgtcgtg gcagtgtgcg
      361 ctgaagacga cgccatcggc gttcggcacc cgcgatcgaa tccgcgtgaa gaagtcgagc
      421 gtctcgtcgg gcgtcgtgat accgaccgtg tcgggaaggt tgatcgtcgt ggcgccgacg
      481 gcgatcaccg attcgacgac cttgcacagg aagtcgaagt cgctgcgcgt cgcgtcctcg
      541 gccgagaact gcacgtcgga ggtgtgctgc cgggcgtagc gcacggagtc gaccgcaacc
      601 tccaggcact gttcgcgcga catccggagc ttggccttca ggtgaaggtc cgacgtcgcg
      661 atgaaagtat ggatccggcc ctgctccgcc ggcttgatcg cccatgccgc cttctcgagg
      721 tcggccttgt gacagcgcgc caggcaggcg atgaccgggc ggcggatctc ggtggagatc
      781 tgacggaccg cctcggcatc ggcgggagag gcaatcggga agccggcctc gatgatgtcc
      841 acgccgagcg cgtccagctg acgcgccagc tgcagcttct cggacgggcg gagagagaac
      901 ccgggcgcct gttcgccgtc gcgcagcgtc gtgtcgaaaa tgcggacgtg ctcgggtgcc
      961 atgggaaacc tccccacgcc agtagacgcg acccgtcgcg ataatgtcaa accgataatt
     1021 attctctttg aatcaccttt tgttatacgc taccggacct ccgcccgctg gcaggacacc
     1081 gtggaactca gtgaattgcg cgtatttctg aaggtcgcct cagaacgcag cttctcgaag
     1141 gcggccatga agcttcaccg gacccagccc gccgtcagcc agtcggtccg gcggctcgag
     1201 gacagcgtcg gggagcgcct gttcgaccgc gcgacgaagg acgcgtcgct gaccgaggcg
     1261 ggccgcctgc tgcgggacta cgccgagcgt ctgctgcgcc tgtccgagga ggcggaaacc
     1321 gcggtcaagg acctccgcga cctgcggcgg ggccgggtcc tggtcggggc gaacgaggcg
     1381 tcggtccacg cggtgctccc tctgatcgcg cgtttccgcg acgggcatcc gctggttcac
     1441 gtggacgtcc gccgcattcc cgcccgccag ctcggagccg aggtcgccca gggcagcctc
     1501 gatttcggcg tgctcacctt tcagccggcc gagccgggcc tcagctcgat cgtgctcggc
     1561 aacgacgagc tcgtcatgct ggtgcacccg tcgcacgaac tggccagccg gcgcgacgtc
     1621 atgctgtcgg aatgcgcgcg acagacggtc atcgcccaca acgacccgtc gcacgtgcgt
     1681 gaacgcgtcc ttcgcctgtt cgagcagcac cacatcccgg ccaacatcct ggtgtcgctc
     1741 ccgagcctcg aaggcatcaa gcgcgccgtc gcgatgcaga tgggcgtagc gctgctcccc
     1801 aggcgctgcg ccgagtcgga gatcgagcga ggggagctgg tcgcgctggc gatgcccgag
     1861 gtccgcatca agcgccaggt gcggctgatc ttccgcaaga gcggagagcg atctcacgcc
     1921 tctgccgcct tcctcgcgat gctcgaggag aagaagaagc c
//
LOCUS       DBNGSH010000493         5106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4631473, whole genome shotgun sequence.
ACCESSION   DBNGSH010000493 DBNGSH010000000
VERSION     DBNGSH010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4631473"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="AC16CY_11325"
     CDS             <1..1058
                     /locus_tag="AC16CY_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3354427.1"
                     /translation="GADVNRRESWHGETALMWAAAENHAEAVTLLASRGADLEARSNV
                     PEFPKVKVDAATMVATALPRGGMTALLLAARQGAGAGVDALARAGADLDAADPDGTTP
                     LVMAIINSHYDVAARLVEHGAGLDRADASGMTPLYAAIDMRHQEPLVNRPLAKPSGRL
                     LPDEVIRLLLDRGANPNARLKTPLLMRQHNGGDPQLGDGATPLMRAAKVSDIATMTAL
                     LDKGADPNLSLRNGTTALMIAASRQGRGAGPEQATVESMRLLIDRGAQVNAVNQNGES
                     ALHVAVARGDALVRFLAERGAALDLKDGSGRTPLDVAMGVPAGGGRGRGRGAAPPGAG
                     VVRESTAALLKELMKGP"
     gene            1086..>5106
                     /locus_tag="AC16CY_11330"
     CDS             1086..>5106
                     /locus_tag="AC16CY_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3354428.1"
                     /translation="MLDQLLGASPALAAVREELARVLARPAAPLKRLPPVLIQGETGT
                     GKGLVAHLIHQMGPRADAAFVDVNCAAIPETLLEAELFGFERGAFTDARQSKAGLLQV
                     AHRGAIFLDEIGLMPEVLQVKLLKALEDRSVRRLGGTRAEPADAWVLAATSEDLVAAI
                     ATRRFREDLYHRLAVVTVQLPPLRERGTDVLLLARHYLDRACHEYGLAARNLSPDAEA
                     ALLAYAWPGNVRELANLMERVALVSEGEQIKADALRLPRAPRIPSASRTGESVDEQIA
                     RLERGRIEEALRAEGGNISRTAARLGLPRNTLRYRMERHGLLDADASSPRRKPEASSQ
                     SAPVQWQRTRVTILQAQLDDALTEPGHERSLALEEFATKIGGFGGRIIELGAAFVRFA
                     FGLDIVEDAPRHAAHAAFAVQRAASAARGSRPPVTVRIALHTEEMPVGRLEDRVELDA
                     DARRTTQGVLDRLLAMTSPEPIVASAATKPFLERGFDLEPVSHDVLCWRVRGLLDGDR
                     HASPFVSRTREIALLEDLLSQVEDGRGQTVLLAGDAGIGKTRLLQELHRRTRGRAAWY
                     QGSAVSFGGSLPLHPLIDLLKDVFSIRVSDDNEVIGERIDQTTDAFGEPLRGSIGFLR
                     SLLSIEAGDPSLAQLDPKLRRAGIFEAIGQLLHALAGVRPVIVVLEDVHWMDQATGEF
                     LALMVESLASSRILLCLTHRTGYTLPFASAAIGTQLTLSPVAPIDSAAMACSILGAHG
                     LSADLQQFIDQKTEGNPFFVEEVLRLLQERGLIVRHGEQAGLLQPTGKVDVPDTIRDV
                     LHGRMGRLDRASRDLLRVAAVIGREFPRRVLERVVDDAALEERLRALRSADLIQNARV
                     WPEVVYAFKHAVTQEVAYAAASEDQRRAEHARIGDAMEQVYADRLSEHVGVLAHHFTQ
                     AQRWHKALEYLLAAAQQAENNYATREALALYDEARRAAEQMAGGVGDPDTIMRIHEAK
                     ARLYFVTSDFQRSAAEGERILPLARLTGNRAREAEALAIIAWASTWGRDLDGAIRFSK
                     EAIAVGEAAGALAVQGRAHFTIGFVRGVTGVLDDSRAALDRAVEISTAAGDAFTRSLS
                     LSGAGLLRNWTGDYGEAERLQSEGRRVALESRQLFPLVFSCFLRGLTLTGKGDYDEAL
                     ASLNEGLSFAERVGDEAIHHRLLNCFGWLYADLGDLEHAEELNATSALVGRRRKDPGT
                     QPNAELNLAEIFAARGERERAQDQYDGVFRYWADPDGSLWMRYRYSIRMFYGMGQLAL
                     ARGDLATARSHCAECLGLATRTGSRKNLVKAWRLAGQIAQAERAWDTAEGHLRTSLDL
                     AVALGNPVQHWKSEIALGQF"
BASE COUNT          790 a         1753 c         1823 g          740 t
ORIGIN      
        1 ggggcgccga cgtcaatcgc cgcgagagct ggcacggcga gaccgcgctg atgtgggccg
       61 ccgccgagaa ccacgccgag gccgtgaccc tgctggcctc gcgcggcgcc gatctcgagg
      121 cgcgctcgaa cgtgccggag tttcccaagg tcaaggtcga tgccgcgacg atggtcgcga
      181 cggcgctgcc acgcggcggc atgaccgcgc tgctcctcgc cgcgcggcag ggagcggggg
      241 ccggcgtgga cgccctggcg cgggcaggcg ccgacctcga cgccgccgac ccggacggca
      301 cgacgccgct cgtcatggcg atcatcaact cgcactacga cgtggcagcg cggctcgtcg
      361 agcacggcgc cggcctcgat cgggccgacg cgtcgggtat gactcctttg tatgcggcga
      421 tcgacatgcg gcaccaggag ccgctcgtca accggccgct cgcgaagcca tcgggccgtc
      481 tgctgccaga cgaggtgatc cggctgctcc tcgatcgcgg cgccaacccg aacgcgcgac
      541 tgaagacgcc gctgctgatg cggcagcaca acggaggcga cccgcagctc ggcgacggcg
      601 ccacgccctt gatgcgcgcg gccaaggtga gcgacatcgc cacgatgacg gccctcctcg
      661 acaagggcgc cgatccgaac ctgtcgctgc gcaacgggac gacggcgctg atgatcgccg
      721 cctcgcgcca gggacgcggc gcgggcccgg agcaggcgac cgtcgagtcg atgcggctcc
      781 tgatcgatcg aggcgcgcag gtgaacgcgg tgaaccagaa cggcgaatcc gcgctgcacg
      841 tcgccgtggc ccgcggtgac gcgctcgtca ggtttctcgc cgagcgcggc gcggcgctcg
      901 acctcaagga cgggtcgggc cgcactccgc tcgacgtcgc gatgggcgtg ccggcgggtg
      961 gtggacgagg ccgcggccgg ggagcagcgc cgccgggagc aggagtcgtg cgcgagagca
     1021 cggcggcgct gctcaaggaa ctgatgaagg gaccctgagg ctccgcactt cgataccata
     1081 agcgcgtgct ggatcaactg ctcggcgcga gcccggcgct ggcggccgtt cgcgaagagc
     1141 tggcgcgtgt actggctcga cccgcggcac cgttgaaacg cctgccgccc gtgctcattc
     1201 aaggcgagac gggaacgggc aaggggctcg tcgcgcacct gatccatcag atgggtccgc
     1261 gcgccgacgc cgcgtttgtc gacgtcaact gcgcggcgat tcccgagacg ctgctcgaag
     1321 cggagctctt cggattcgaa cgcggcgcgt tcaccgacgc caggcaatcc aaggccgggc
     1381 tgctccaggt cgctcatcgc ggcgcgatct tcctcgacga gatcggcctc atgccggagg
     1441 tccttcaggt caagctgctc aaggcgctcg aggatcgatc cgtccggcgc ctgggcggca
     1501 cgcgcgccga gccggcagac gcctgggtgc tcgcggcgac gagtgaagac ctggtggccg
     1561 caatcgccac caggcggttt cgcgaggacc tctaccatcg gctcgccgtc gtgaccgtgc
     1621 agctgccccc gctccgcgag cgcggaacgg acgtcctgct gctcgcgcgc cactacctcg
     1681 accgcgcgtg ccacgagtac gggctggcgg ccaggaacct gtcgccggac gcggaggcgg
     1741 ccctgctggc ctacgcgtgg cccggcaacg ttcgcgagtt ggccaacctg atggagcgcg
     1801 tggcgctcgt ctcggagggc gagcagatca aagccgacgc gctgcggctg ccgcgcgcgc
     1861 ctcgcatccc gtcggcgtcg cgcacgggcg agagcgtgga cgagcagatc gcccggctcg
     1921 agcgcggtcg catcgaggag gcgctgcgag ccgaaggcgg aaacatctcg cgcacggccg
     1981 cccggctcgg cctgccgcgc aacacgctcc gctaccggat ggagcgccac ggtctactcg
     2041 acgccgacgc ctcgagcccg cgccgcaagc ccgaagcgtc gagccagtcc gcgccggtgc
     2101 agtggcagcg cacccgtgtc acgattctgc aggcacagct cgatgacgcg ctcaccgagc
     2161 ccggtcacga gcgaagcctg gcgctggagg agttcgccac gaagattggc ggcttcggcg
     2221 gccgcatcat cgagctcggc gccgctttcg tccggttcgc cttcggcctg gacatcgtcg
     2281 aggacgcccc acgccacgcg gctcacgcgg ccttcgcggt gcagcgcgcg gcttccgccg
     2341 cccgcggctc gcgcccgccg gtgacggtgc gaatcgccct gcacaccgag gagatgcccg
     2401 ttggccggct cgaggatcga gtcgagctcg acgccgacgc gcgccggacc acgcaaggcg
     2461 tcctggaccg cctcctcgcg atgacgtccc ccgagccgat cgtcgcgtcg gcggcaacca
     2521 agccgttcct ggagcggggg ttcgacctcg agcctgtttc tcacgacgta ctctgctggc
     2581 gggtcagggg gctgctcgac ggcgaccgtc acgccagtcc gttcgtgtcg cgcacccggg
     2641 aaatcgcgct gctcgaggat ctgctctcac aggtcgaaga cggacggggc cagacggtgc
     2701 tgctcgccgg cgatgccggc atcggcaaga cgcgtctcct gcaggagctt caccggcgga
     2761 cgcgggggcg cgccgcctgg tatcagggat cggccgtgtc cttcggcggc tcgctgccgc
     2821 tgcacccgct catcgatctg ctgaaagacg tgttctcgat tcgcgtcagc gacgacaacg
     2881 aggtcattgg cgagcggatc gaccagacga cggacgcgtt cggagagccg ctgcgcggat
     2941 cgatcggctt cctgcgatcg ctgctgtcga tcgaggcagg cgacccttcg ctggcccagc
     3001 tcgatccgaa gcttcgccgg gccggcatct tcgaggcgat cggccagctg cttcatgcgc
     3061 tcgcgggcgt tcgcccggtg atcgtcgtgc tcgaggacgt gcactggatg gatcaggcca
     3121 cgggagagtt cctggcgttg atggtcgaga gcctcgcgtc cagccggatt ctgctgtgcc
     3181 tcacgcacag gacgggatac acgctgccgt ttgcctcggc ggcgatcggc acgcagctga
     3241 cgctgtcgcc ggtcgcgccc atcgacagcg cggcgatggc ctgctccatc ctgggtgcgc
     3301 acggcctgtc ggccgacttg cagcagttca tcgaccagaa gaccgaaggc aatccgttct
     3361 tcgtcgagga ggtcctgcga ttgctgcagg agcgcggact gatcgtccgg cacggcgagc
     3421 aggccggcct gttgcagcca acgggcaagg tcgatgtccc cgacacgatc cgggacgtgc
     3481 tgcacgggcg catgggccgg ctcgatcggg cgtcgcgcga tctgctgaga gtcgccgccg
     3541 tcatcggccg ggagttcccg cgtcgagtcc tcgagcgtgt cgtggacgat gcggctctcg
     3601 aagagcgcct gcgcgcattg cgatcggccg acctgataca gaacgcacga gtctggccgg
     3661 aggtcgtcta cgcgttcaag cacgcggtga cccaggaagt ggcctacgcc gccgcgagcg
     3721 aggatcagcg tcgagccgag cacgccagaa tcggcgacgc gatggagcag gtgtacgccg
     3781 accgcctctc cgaacacgtc ggcgtgctgg ctcatcactt cacgcaggcg cagcgctggc
     3841 acaaggcgct cgaatacctg ctggccgcgg cgcagcaggc ggaaaacaac tacgccaccc
     3901 gcgaggcgct cgcgctgtac gacgaggccc gtcgcgcagc cgagcagatg gcgggcgggg
     3961 tgggcgaccc ggacaccatc atgcggatcc acgaggccaa ggcgcgactg tacttcgtga
     4021 ccagcgattt ccagcgttcc gcggccgaag gggagcgaat ccttcccctg gcgcgcctca
     4081 caggcaatcg cgcccgcgaa gccgaggcgc tcgcgatcat cgcctgggcc tcgacctggg
     4141 gccgcgacct cgacggggcc atccgcttct cgaaggaggc gatcgcggta ggagaggccg
     4201 ccggcgcgct ggcggtacag gggagagcgc acttcacgat cggcttcgtc cgcggggtca
     4261 cgggcgtgct cgacgacagc cgggccgctc tcgaccgcgc cgtggagatc agcactgccg
     4321 ccggagatgc gttcactcgc tccctgtcgc tgtcgggcgc ggggctgctc cgcaactgga
     4381 ccggcgacta cggggaggcc gaacgcctgc agagcgaagg ccgccgcgtg gcgctcgaga
     4441 gccgtcagct ctttcccctg gtgttcagtt gtttcctgcg cgggctgacg ttgacgggga
     4501 agggcgacta cgacgaggcg ctcgcctcgt tgaacgaagg gttgtcgttt gccgagcgcg
     4561 tcggagacga agcgattcat catcggctgc tcaattgctt tggatggctc tacgcggacc
     4621 tcggcgatct cgagcacgcc gaggagctca atgcgacgag cgcgctggtc ggccgccgcc
     4681 gcaaggatcc gggaacgcag ccgaacgcgg agctgaatct ggccgagatc ttcgcggcca
     4741 ggggcgaacg cgagcgcgcg caggatcagt acgacggtgt cttccgctac tgggccgatc
     4801 ctgacggcag cctgtggatg cgctaccgct attcgatccg gatgttctat ggcatgggac
     4861 agctggcgct cgcgcggggc gacctcgcga cagcacggtc gcactgcgcg gaatgtctcg
     4921 ggctggccac tcgaacaggg tcgcgcaaga acctcgtgaa agcgtggcgg ctcgccggcc
     4981 agatcgcgca ggccgagcgg gcgtgggaca cagcggaagg ccacctgcgg acgtcgctcg
     5041 atctggcagt cgcgctgggc aaccccgtcc agcactggaa atcggagatc gcgctgggac
     5101 agttcc
//
LOCUS       DBNGSH010000494         8225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1744760, whole genome shotgun sequence.
ACCESSION   DBNGSH010000494 DBNGSH010000000
VERSION     DBNGSH010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1744760"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2910
                     /locus_tag="AC16CY_11335"
     CDS             <1..2910
                     /locus_tag="AC16CY_11335"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3354429.1"
                     /translation="GTYTVTFTLSGFSGIRREGIELAGAFVATVNAEMRIGGVTETIT
                     VSGEAPVVDVTSTRNQQVISNETISEIPSSRQYSAFTHLIPAINVQQNDFSGSNPALY
                     SVFQIHGGRRNEGQVLVDGMNGGYQGMGVSGYVPEVGTAQEVVFSLAGGLGEATTGGP
                     QMNIIPKQGGNNFSGGIFISGTGDALQGSNLTPEIQAQGLTATNSIKQLWDINPSFGG
                     PIVRDKLWFYGTYRYQVSRQNVASMWVNRNAGDPTSWAYDPVLEDQAVADGNWNNGSA
                     RITWQVTPRNKFSVWSSVQYHCLYCEGGGDGTGLGFGAMITSPEATATNENHPSWLTQ
                     LSWTSPFTNRVLLEANAQLGPYFWWGSRQKNSYDDTMIPVQETAGPYPGINYRAPVGL
                     GAGAWSEHTGLTNILQGAVSYITGSHSLKVGYRFHHNLSTFPANFYNNTGLRYTLTGG
                     IPSQVTAYAEGGSQQEQHQTMNAFYAQDRWTVNRLTVQAGLRLEHLSDYFPEQVMGPN
                     RFLPTAVVFPAEEGPLNQKDLMPRFGVSYDLFGNGKTALKFFTGRYVTTFNTVDEWAN
                     FSPAGISRFQSTDQRGWDDFAYPVGDPRRGDFVADCNFLDPAANGECGLGSAFFGKAL
                     PSNSIDPAFVDGWNTREYSWDLNAGITQELVPRVSLQVDYIRRSWGNLGVTVNDAWTA
                     ADFDTFHYTVPQDSRLPGGGGYTLTYRDVKPGKIQQISNRLTFANEVGGAFNNFNGVD
                     VTVNARLRDVTIQGGTSTGNVVEDSCGVVAENPEYYIFGPWGGTGGFLDTFLGGIGQW
                     PQEFCHRETGWKTNIKGLASYTVPKIDVLLSGTFKSLPYPGNEFPSVQSQSLGGQTLG
                     LFLGIPGVDSTDLGRPLSSGIPIQFLNLVHPGESYGDRLTSIDLRFGKILRYGNSRAS
                     INFDIYNLFNSNTTEVYQRNYSAPTAPGAVPRSTYLDPLQIMSARFFKISGQIDF"
     gene            3102..3431
                     /locus_tag="AC16CY_11340"
     CDS             3102..3431
                     /locus_tag="AC16CY_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3354430.1"
                     /translation="MARTPAALLQGTLDLLILKALSTGELHGLGVSRRIDQMTGGTFV
                     VQPGSLFPALHRLEEAGWLEASWDASEHNRRAKFYALTKAGRRQLSQETADWNRVSLA
                     IARALKA"
     gene            3440..6196
                     /locus_tag="AC16CY_11345"
     CDS             3440..6196
                     /locus_tag="AC16CY_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3354431.1"
                     /translation="MSRLWSLWRNLWRKEQVERDLDDEMRATLDLLAEEKVRAGHAPD
                     DARRIASLELGGVESVKQQVRDARRGAVLDTVVRDVRHAARLLWRNPLFTLTAALSLA
                     IGIGATTAIFTVANGLLLRAATGVADPTSLIDIVRRDPRQGPGVEEMSYPDLVDVRQH
                     ATLLDEVFGYQLTLTPASLNVDDSSSAVFASVVTTNYFRALGVTAAAGRVFDAGDVEQ
                     AGASPIVVLSHEFWLRRFDGDSGVVGRGVRINNVPMTIAGVAAGGFRGVSVVAPDLWV
                     PVSAIAALAPEGGGVELTDRRVPWLMLGARLKPGVSRAQASAELAGIGAAIQRDTPSN
                     PFIPEGAHEVDSSSLVWSAEVASPIPYGLRVLAAGFLGLLMTLVAVVLVIACANLGGV
                     LLAKATGRRREIAVRTAVGAGRARLVRQLLTETVLLFALGGIAGLALARGITTLLISL
                     LPEFPLPVSLSVPLDLRVVAFALVLSFIAAILAGLAPALHASKSDVVTALKDDVQGPV
                     DRLRLRNTFVVAQVAFSVLLVVVAALLVRAFDNVISVKHGFDPRDVEVASLDLTMGGY
                     TNATGGEVVRRLRERVRAIPGVARASIADHAPAPGGRSFGSISVPGAVQPGGRSDTFT
                     NWTLVEPDYFRTVGIPLMAGRDFTDADGPGAELVVIVGQRTAKRLWPDRDPIGQLVSV
                     RPPQGPQAATLQPRDPGTRVPDRQLRVVGVVGDLNFGTQGDSAPLSIYVPFQQKYLAQ
                     ITILARRKTEGQSLAQELRSAVASIDPNLPVLTTELLERQNNGPVQTQLRVAATVAGS
                     VGIIGLFLAGIGIYGVTAYAVTRRTREIGIRLSLGASSREVIGLVLRQGMRLVVIGSA
                     IGLALGIGAGKLLSGRRFGIPQADPAVLIGAGLLFAVVGLVACYIPVRRAASIRATEA
                     LRYE"
     gene            6278..7765
                     /locus_tag="AC16CY_11350"
     CDS             6278..7765
                     /locus_tag="AC16CY_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HIR3354432.1"
                     /translation="MNDDARLREHVRPPGWRNPSPAPRYDLVVIGGGTAGLVCAAGAS
                     GLGARVALVERALLGGECLNTGCVPSKSLLRSARAASEMHGAASLGMTAAGPPDFGAV
                     MARLRARRADLAPHDSAERLKSLGVDVFFGTAAFSSPRSVTVRSNGSNAADDSGVVLR
                     FRKAVIATGSRPLVPDIPGLAGTPFLTSDTLFDLTDRPKSLIVIGAGPTGCEMAQAFA
                     GLGTRVTLVESSPHVLPNEDAEAGAILGDRLRKDGVELRLGAAIGAVRWSQGCFVLDG
                     AGPAMSAEAVLVATGRTPCADGLDLDAAGVAHDAKGVTADDFLRTTNRRVFAAGDVCS
                     RLKFTHMADAMARIVIQNALFFGRRRVSALVVPSCTFTSPQVAHVGRTVAEASASGAD
                     TITIPLSSVDRAVVDEATAGFLRIHHTGGRIVGATIVAPEAGEMIGTIVLAMQHGHGL
                     ADLASVSFPYPTVSLALRQAGDAYRRSRLTPAVRALLRYYLSAFR"
     gene            7762..>8225
                     /locus_tag="AC16CY_11355"
     CDS             7762..>8225
                     /locus_tag="AC16CY_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354433.1"
                     /translation="MTALLVALILWQPSACAEDSVLARAAQRADAFDLDGAIASLAAS
                     REAGCRAADVPYWYLRGLLAAREAYRYGGSPESLEPVRAAIAALNARPSRTSDVAQSV
                     LEAAAAAAQSEREEMALRLDHALRLETQLRAGGAPGAPIVSAHEAAGDLWLRV"
BASE COUNT         1316 a         2692 c         2786 g         1431 t
ORIGIN      
        1 gggacctata cggtcacgtt tacgctgtcc gggttcagcg ggatcaggcg cgaagggatc
       61 gaactggccg gcgcgttcgt cgcgaccgtc aacgcagaga tgaggatcgg gggcgtcacg
      121 gagacgatta ccgtgagcgg cgaagcgccg gtcgtcgacg tgacgagcac gcgcaatcag
      181 caggtgatca gcaacgagac gatctccgag attccctcgt cccggcagta ttccgcgttc
      241 acgcatctca tcccggcgat caacgtccag cagaacgact tctcgggctc caaccccgca
      301 ctctacagcg tgtttcagat ccacggcggg cgcaggaacg aggggcaggt cctcgtggac
      361 ggcatgaacg gcggctacca gggcatgggc gtgtcgggct acgtgcccga ggtcggtacc
      421 gcgcaggaag tcgtgttcag cctggcgggc gggcttggcg aggcgaccac cggcggcccg
      481 cagatgaaca tcattcccaa acagggcggc aacaacttct ccggcggcat cttcatcagc
      541 ggcacgggcg acgccctcca gggcagcaac ctcactccgg agatacaggc ccaggggctc
      601 accgcgacga actcgatcaa gcagctgtgg gacatcaacc cgtcgttcgg cggcccgatc
      661 gtccgtgaca agctgtggtt ctacgggacg taccgctacc aggtcagccg gcagaatgtc
      721 gccagcatgt gggtcaacag gaacgccggc gatccaacga gctgggccta tgaccctgtc
      781 ctggaagacc aggccgtcgc cgacggcaac tggaacaacg ggtccgcgcg gatcacgtgg
      841 caggtgacgc cgcgaaacaa gttcagcgtc tggtcgagcg tgcagtatca ctgcctgtac
      901 tgtgaaggcg gcggcgacgg caccgggctt ggcttcgggg cgatgatcac ctcgcccgag
      961 gcgacggcga cgaacgaaaa ccatccgagc tggctgacgc aattgagctg gacgtcgccg
     1021 ttcaccaatc gcgtgctgct cgaggccaac gcgcagctcg ggccctactt ctggtggggc
     1081 agccgtcaga agaactccta cgacgacacg atgattccgg tacaggagac cgcaggtccc
     1141 tatccgggca tcaattaccg ggcgccggtc ggtctcggtg cgggggcgtg gtccgagcac
     1201 accggattga cgaacatcct ccagggggcg gtctcgtaca ttaccgggtc gcactcgttg
     1261 aaggttggtt accgtttcca ccacaacctg tctaccttcc cggcgaactt ctacaacaac
     1321 accggactca ggtataccct cacgggcggc attcccagcc aggtgacggc gtacgctgaa
     1381 ggtggatcgc agcaggaaca gcatcagacg atgaatgcgt tctatgccca ggatcgctgg
     1441 acggtcaatc gcctcacggt gcaggccggg ctccgtctcg agcacttgag cgattacttc
     1501 cccgagcagg tgatgggtcc aaaccgcttc ctgcccaccg ccgtggtctt cccagccgaa
     1561 gaaggcccgc tcaatcagaa ggacctgatg cccaggttcg gcgtgtcata cgacctgttt
     1621 ggcaacggga agacggccct caaattcttc acgggccgat acgtgacgac gttcaacacg
     1681 gtggacgagt gggcgaactt cagccccgcc ggaatcagcc gtttccagag cacggaccag
     1741 cgaggctggg acgacttcgc gtatccggtc ggcgatcccc gcagggggga tttcgtcgcc
     1801 gattgcaact tcctggatcc ggcggccaac ggcgagtgcg gactcgggag tgcgtttttt
     1861 ggaaaagccc tcccttcgaa ctcgattgac cccgcgttcg tggatggctg gaacacgagg
     1921 gagtacagct gggatctgaa cgccggcatc acgcaggagc tcgtgccgag agtgtcgctc
     1981 caggttgact acatccgccg gagctggggg aacctggggg tcaccgtcaa cgatgcgtgg
     2041 acggcggcgg atttcgacac cttccactac accgttccgc aggacagccg cctgccgggc
     2101 ggcggcggct acacgctgac ctaccgcgac gtgaagccgg ggaagatcca gcagatcagc
     2161 aaccggctca ccttcgccaa cgaagtcggc ggagccttca acaacttcaa cggcgttgac
     2221 gtgaccgtga acgcgcgtct gcgcgacgtc acgatccagg gcggcacgag caccggcaac
     2281 gtcgtcgagg actcgtgcgg cgtggtcgcg gagaaccccg agtactacat atttggtccc
     2341 tggggcggca cgggcgggtt cctcgacacg ttcctcggtg gcatcgggca gtggccgcag
     2401 gagttctgcc atcgcgagac gggatggaag acgaacatca aggggctggc ctcgtacacg
     2461 gtgccgaaga tcgacgtgct gctcagcggg acgttcaaga gcctgccgta ccccgggaac
     2521 gaattcccgt ccgtgcagag tcagagcctc ggtggccaga cgctcgggtt gttccttggc
     2581 attccggggg tggacagcac ggaccttggc cgcccgctgt ccagtggcat tcccatccag
     2641 ttcctgaacc tcgtccaccc cggcgagtcg tatggcgatc gcctgacctc gatcgatctc
     2701 cggttcggca agatcctgag gtacggcaac tcgcgggcgt cgatcaactt cgacatctac
     2761 aatctgttca attcaaacac gacggaggta taccagcgga attactcggc tcccacggct
     2821 ccgggagcgg taccgcggtc cacctatctc gatccgctgc agatcatgtc cgcgcggttc
     2881 ttcaagatca gtggtcagat cgatttctaa gcgggaatag ggcactccga atccccgcct
     2941 ccggccttcg agccgggggc ggggccgttc cgcggtccat ccttcgcccg cctcacattc
     3001 ctattgacat tcgataggaa ccagccaacg atacgtcttc gtatagaacg tctgtacgta
     3061 gcgtccggct ttagccggac ctgaccacgg agcgcgatcc catggcacgc acccccgcgg
     3121 ccctcctgca gggcacgctc gacctgttga tcctgaaggc cctgtccacc ggcgagctgc
     3181 acggactagg cgtctcccgt cgcatcgacc agatgaccgg gggcaccttc gtcgtgcagc
     3241 cgggctcgct ctttccggcg ctgcaccggc tcgaggaggc cggttggctc gaggcgagct
     3301 gggacgcgtc ggaacacaac cgccgcgcca agttctacgc gttgacgaag gcgggccgcc
     3361 gccaactgag tcaggagacg gccgactgga atcgcgtctc gttggcaatt gcgcgtgccc
     3421 tcaaagccta ggagcaacga tgtcccgtct gtggagcctg tggcgaaatc tctggcggaa
     3481 ggaacaggtc gagcgcgatc tcgacgacga gatgcgggcg acgctggacc tgctcgctga
     3541 agaaaaggtc cgcgcggggc acgcccccga tgacgcgcga cgaatcgcgt cgctcgagct
     3601 gggcggcgtg gagtcggtca agcagcaggt gcgcgacgcg cggcgcggtg cggtcctcga
     3661 cactgtcgtc cgggacgttc ggcacgccgc ccgcctgctc tggcgcaatc cgctgttcac
     3721 gctcacggct gcactctcgc tcgccattgg catcggcgcc acgacggcca tcttcaccgt
     3781 cgccaacggc ttgctgttgc gcgcggccac gggcgttgcg gatcccacga gtctcatcga
     3841 catcgtccgg cgcgatcccc gtcaagggcc tggcgtcgaa gagatgtcgt acccggacct
     3901 ggtcgacgtg cgtcagcacg ccacgctgct cgacgaggtg ttcgggtacc agctcacgct
     3961 gacgccggcc agcctgaatg tggacgactc gtcttccgcc gtctttgcga gcgttgtcac
     4021 gacgaactat ttcagggcgc tcggtgtgac ggcggccgcg gggcgcgtct tcgacgcggg
     4081 cgacgtcgag caggcgggtg ccagtccgat tgtcgtcctg agccacgagt tctggctgcg
     4141 ccggttcgac ggcgattccg gcgtcgtggg tcgtggcgtg cggatcaaca acgtgcccat
     4201 gacgatcgcc ggtgtcgcgg ccggcggctt tcgcggcgtc agcgtggttg ccccggatct
     4261 gtgggttccc gtctcggcga tcgcggcgct cgcgcccgag ggcggcggag tcgaattgac
     4321 cgatcgacgc gtcccgtggc tgatgctggg ggcgcggctg aagccgggtg tgtcacgtgc
     4381 ccaggcctcg gcggaactgg cgggcatcgg cgcagccatc cagcgggaca cgccttcgaa
     4441 ccccttcatt ccggagggcg cccacgaggt cgattcatcc tctctcgtct ggagcgccga
     4501 ggtcgcctcc ccgattccct acggcttgcg agtgctggcg gctgggtttc tcgggctgct
     4561 gatgacgctc gtggccgtcg tcctcgtgat cgcctgcgcg aatcttggcg gtgtcctgct
     4621 cgcgaaagcc accggccgcc ggcgggagat cgccgtgcga accgcggtcg gggcggggcg
     4681 cgccaggctg gtgcgccaac tgctcaccga gacggtgctc ctcttcgcgc tcggaggcat
     4741 cgcgggcctc gcgctggcgc gtggcatcac gacgctcctg atttcgctgc tgcctgaatt
     4801 cccgcttccg gtcagccttt cggtgccgct cgaccttcgc gtcgtggcgt tcgcgctcgt
     4861 gctctccttc attgccgcca tcctggccgg cctggccccg gcgctccacg catcgaagtc
     4921 cgatgtcgtg acggcgctca aggacgacgt gcaggggccg gtcgatcgct tgcgcctccg
     4981 caatacgttc gtcgtcgcgc aggttgcctt cagcgtcctg ctcgtcgtgg ttgcggcgct
     5041 cctcgttcgc gcgttcgaca acgtcatctc cgtcaaacac ggcttcgatc cgcgggacgt
     5101 cgaggtcgcc tcactcgatc tgacgatggg gggctatacc aacgcgaccg gcggcgaggt
     5161 ggtgcgccgc ctgcgcgagc gggtccgcgc catcccgggc gtcgcacgtg catcgatcgc
     5221 cgatcacgcc ccagccccgg ggggacgaag tttcggaagc atctcggtcc cgggcgccgt
     5281 gcagccgggc gggaggtccg acacgtttac gaactggacg cttgtcgagc ccgactactt
     5341 ccggaccgtc ggcatcccgc tcatggcggg ccgtgacttc accgacgccg atggtcctgg
     5401 cgccgagctc gtggtgatcg tcggccagcg gacggcgaag cgcctctggc ccgaccgcga
     5461 tccgatcggg cagctcgtct ccgtccgccc ccctcagggc ccgcaggcgg cgacgctgca
     5521 gccgcgcgat ccgggcacgc gcgtaccgga caggcagctc cgtgtcgtcg gcgtcgtggg
     5581 tgatctgaac ttcggcacac aaggcgattc ggcaccgctc agcatctatg tccccttcca
     5641 gcagaagtac ctcgcccaga tcacgatact ggctcgccgg aagacagaag ggcaaagcct
     5701 ggcgcaggaa ctccggtcgg ccgttgcctc catcgatccc aatctccccg tgctcacaac
     5761 cgagttgctc gagcggcaga acaacgggcc ggtgcagaca cagcttcgcg ttgcggcgac
     5821 agtggccggt agcgtcggca tcattggtct gttcctggcc ggcatcggaa tctacggtgt
     5881 cacggcctac gcagtgacac ggcgcacgcg tgagatcggg attcgtcttt cgctgggtgc
     5941 atcgagcagg gaagtgatcg gcctcgttct gcgtcagggc atgcgcctcg tcgtcatcgg
     6001 ttcggcaatt ggccttgccc tcgggatcgg agccgggaag ctgctgtccg ggcgacggtt
     6061 cggcatcccg caggccgacc ctgccgtcct catcggtgcg ggcctgctgt ttgccgtcgt
     6121 cgggctcgtc gcgtgctaca taccggtgcg tcgcgccgcc agcattcgag cgacggaggc
     6181 gctgcgatac gaataagggg gaagggggaa gggggaaggg gcaggggcaa gggggcaggg
     6241 ggcagggggc agggaggagt cgagagaaga taggcgtgtg aacgacgacg cgcgtctgcg
     6301 cgagcatgtc cgtccaccgg gatggcgcaa tccatctccg gcccccaggt acgacctcgt
     6361 cgtcatcggc ggcgggacgg ccggcctcgt ctgcgcggcc ggcgcgtcag ggctcggcgc
     6421 ccgcgtggcg ctcgtcgagc gagccttgct cggcggcgag tgcctcaaca ccggctgtgt
     6481 cccgtcgaaa tcgttgttgc gatcggcacg agcggcgtcg gaaatgcacg gggccgcctc
     6541 actcggcatg acggcggcgg ggccgcctga tttcggcgcg gtcatggcac ggctccgtgc
     6601 caggcgcgcg gatctggcac cgcacgactc cgccgagcgc ttgaaatcgc tcggcgtgga
     6661 cgtgttcttc gggacggccg cattctcgag tccgcggtcg gtgacggtcc gctcgaacgg
     6721 atcgaacgcg gcggacgatt cgggggtcgt gcttcgtttt cgcaaggccg tcatcgccac
     6781 cggaagccgg ccgttggtgc cggacatccc gggattggcc ggcacgccgt tcctgacgag
     6841 cgacacgctc tttgacctca ccgatcggcc gaagtcgctg atcgtgatcg gcgccgggcc
     6901 gacgggctgc gagatggcgc aggcgttcgc ggggctgggc acgcgcgtca cgctcgttga
     6961 atcgtcgccg cacgtgctgc cgaacgagga cgccgaggcc ggtgccattc tgggcgatcg
     7021 tctgaggaaa gatggcgtcg agctccggct gggggccgcg attggagcgg tacgatggtc
     7081 gcaaggctgt ttcgtgctgg atggcgcggg acctgcgatg tcggcagagg cggtactcgt
     7141 cgcaaccggg cgaacgcctt gcgctgacgg cctggatctc gacgcagccg gggtggcgca
     7201 cgacgcgaaa ggcgtgacgg ccgatgactt tcttcgaacc accaaccgtc gcgtcttcgc
     7261 ggccggcgac gtctgctcga ggctcaagtt cacacacatg gccgatgcca tggcgcgcat
     7321 cgtcattcag aacgcgctgt tcttcgggcg ccggcgtgtc tccgcgctcg tcgtcccctc
     7381 gtgcacgttc acgtcgccgc aggtcgccca tgtcggacgt accgtcgccg aggcttccgc
     7441 gagcggggcc gacacgatca cgattccgct gtcgtcggtc gatcgggcgg tcgtggacga
     7501 ggccaccgcc ggcttcttgc gcatccatca cacgggcggg cgtatcgtcg gcgcgacgat
     7561 tgtcgctccg gaggctggcg agatgattgg cacgatcgtg ctggcgatgc agcacggcca
     7621 cggcctggct gatctcgcgt ccgtctcctt tccctatcca acagtgtcgc tggcgctccg
     7681 acaggcgggt gacgcctacc gacggtcgcg tctgacgcca gcagtccgtg cgctgctgcg
     7741 ctactatctg agcgccttcc gatgacggct ctgctcgtgg cgctgatcct gtggcagcct
     7801 tcggcgtgcg cggaggattc cgtgctcgcc agggcagcac agcgcgccga cgcgttcgat
     7861 ctcgatggag ccatcgccag cctggcggcg agccgcgagg ctggctgccg cgcagctgat
     7921 gttccgtact ggtatctgcg cgggttgctc gccgcgaggg aggcgtatcg ctacggggga
     7981 tcgcctgaat cgctcgagcc cgtacgggcg gcgatcgccg cgttgaacgc gcggccgtca
     8041 cgaacctccg acgtcgcgca atccgtgctg gaggcggccg ccgctgcggc ccaaagcgag
     8101 cgcgaagaga tggcgctgcg gctcgaccac gcgcttcgcc tcgagacgca gctgcgtgcc
     8161 gggggggctc cgggggcgcc gatcgtcagc gcccacgaag ccgcgggcga cctgtggctt
     8221 cgcgt
//
LOCUS       DBNGSH010000495         3973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3951945, whole genome shotgun sequence.
ACCESSION   DBNGSH010000495 DBNGSH010000000
VERSION     DBNGSH010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3951945"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="AC16CY_11360"
     CDS             complement(<1..1170)
                     /locus_tag="AC16CY_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3354434.1"
                     /translation="MRRQGCIAILLIAMVASVSGQSGQKPKKFLGGPLVIEDQGSFFI
                     GGVPKITDFAGAPGPPAPGAPPAQAPTPQQITIGQMYVQFQIPAKKYDGGWPVIMVHG
                     STHTGACLEATPDGREGWYPYFVRNGVPSYVVDQAGRGRSGFDQSVIHEAEARLASGD
                     TKTAADLLPGFGRITDNGAWTAWFGHLVPQGSTVLNGTLVPHGHPQDPNPKPDAYQHV
                     APLFPIDSVDPSIAARTGAIGPAPGGSSAYYALDYYKQLVPNAEVTLPGSTCASCNPT
                     ALSPANTWTPQNLAALVERLGGAIVVTHSQSGIMGHHMTRILKERGHLDLLKGLITLE
                     GSCSLSNSGLTAADFDAIPYMALKGDYAGTSMVCQETVDAINARRAAKQGTAKAEY"
     gene            1407..2186
                     /locus_tag="AC16CY_11365"
     CDS             1407..2186
                     /locus_tag="AC16CY_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354435.1"
                     /translation="MKGLWAVVALVCLMVAAPAAQWLKHPTPGIPRMPDGRADLSAPA
                     PRAHDGKPDLSGLWAPAAILIGDIASRLPPGSVPFQPWAEKLYKERLANQGLDDPTAN
                     CIVGGVPRSDLVPYPFKVLQQPGLTVILYEAVHSYRQIFSDGRPLPTDPNPAWFGYSV
                     GRWEKDTFVVESSGFNDRGRLDNNGHPITEKLRVTERFTRKDFGHMDVVITIDDPGAY
                     TRPWDVTLPLILRPDTELLEYICNENNKFFEIIPKQPAGSR"
     gene            2284..2775
                     /locus_tag="AC16CY_11370"
     CDS             2284..2775
                     /locus_tag="AC16CY_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3354436.1"
                     /translation="MRTAIVFVVGLVIGSALAPGFAQDTRIPGVNNMNHVAIVVPNLV
                     DAIAFYTQKMGFKEGFTVKNDQGQPTLSYIQVSRDTFIELQPANANRQPGLSHFGLHV
                     ADIKTYVAELKKRGVTVTDPRQGRGDSMAANATDPGGMNIEIFQFGPDSLQGQAISSW
                     KQP"
     gene            2772..>3973
                     /locus_tag="AC16CY_11375"
     CDS             2772..>3973
                     /locus_tag="AC16CY_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354437.1"
                     /translation="MKSLAGAVAVLAGSLSLVLVAQSPDVLENAARSMGAPGLQSIQY
                     SGRGSVYLLGQAPAPGAPWPRFELAKYIASVDYARNAMREEQVRRDVERPPRGGGAGP
                     FNPATGQGGIRPIPGDVSQNIVRDGRTEAGLIQIALTPHGFLKAAVANKASVTTSTAR
                     GRTSHAISFAIGRHVLTGTIDDRGLVERVETRIANAVLGDMLVEALFADYKDHGGVKF
                     PSRITHRQGRHAVLDLSVFDVQPNGAAAAPVNTPVPPAAGTAAAPGGADAEKIADGVW
                     FLTGGNPMSVLVEFSDHVVVIEAPQGERRSEATIAAVKTLLPSKPIRYVVNTHHHFDH
                     AGGIRGFVAAGIPVVTHQANKPYFERIFKNAFTLEPDRLARAPRPAVLETVADKRVFA
                     DAAMTLEL"
BASE COUNT          674 a         1317 c         1323 g          659 t
ORIGIN      
        1 atactccgcc ttcgccgtgc cctgcttggc tgcgcgccgc gcgttgatgg cgtcaacggt
       61 ctcctggcac accatgctgg ttcctgcgta gtcgcccttg agcgccatgt acgggatggc
      121 gtcgaagtcg gccgccgtga ggccgctgtt ggacagcgag caggagcctt cgagcgtgat
      181 caaccccttg agcagatcga ggtggccccg ctccttcaga atgcgggtca tatgatgccc
      241 catgatcccc gattgcgagt gggtcaccac gatggcgccg cccagccgtt ccaccagcgc
      301 cgccaggttc tgcggcgtcc aggtgttggc cggtgagagc gcagtcgggt tgcacgaggc
      361 gcaggtcgag ccgggcagcg tgacctcggc attagggacg agctgcttgt aatagtcgag
      421 cgcgtaatac gcgctggagc cgccgggtgc gggtccgatg gcgccggtgc gcgcggcgat
      481 gctcgggtca accgagtcga tggggaagag cggcgccacg tgctggtagg cgtccggctt
      541 cgggttcggg tcctggggat gaccgtgcgg caccagcgtg ccgttgagga cggtcgagcc
      601 ctgcgggacg agatgcccga accacgccgt ccaggcgccg ttgtcggtaa tccggccgaa
      661 gcccggaagg agatccgccg cggtcttggt gtcgccgctt gccagcctcg cctcggcctc
      721 gtgaatcacc gattgatcga atccggagcg tccacgtcct gcctgatcga ccacgtagct
      781 cggcacgccg ttgcggacga agtacggata ccagccctca cgcccatccg gcgtcgcttc
      841 gagacaggct cccgtgtgcg tcgacccgtg caccatgatg accggccatc cgccgtcgta
      901 cttcttcgcc gggatctgga actggacgta catctgcccg atcgtgatct gctgcggcgt
      961 cggcgcctgc gccggcgggg cgccgggcgc gggtggccct ggagcccccg cgaagtcggt
     1021 gatcttcgga acgccgccga tgaagaagct tccctggtcc tcgatgacga gtgggccgcc
     1081 caggaacttc tttggcttct ggcctgattg ccccgagaca ctcgcgacca ttgcgatcaa
     1141 caggatggca atacacccct gacgtctcat tgagtccccc ttgcccgcgc tggctgaagc
     1201 cagcgctcta cgatcggccc cgtcttcaac gcgcatccgt agagcggggg cttcagcccc
     1261 cgcgaacggt ctacgatcgg cctcgtcttc aacgcgcatc cgtagagcgg gggcttcagc
     1321 ccccgcgaac ggtcgcgacg gacccagagt gtatgaggtt tgacaaccct tgcgccacag
     1381 gcataatcgg tcgggaggaa tcgcgaatga aaggcctttg ggcagtggtt gcgcttgtct
     1441 gtctgatggt tgccgcgccg gcggcgcagt ggctcaagca tccaaccccg ggtattcccc
     1501 ggatgccgga cggcagagcc gacctgtccg cgccggcgcc gcgggcccac gacggcaagc
     1561 ccgatctgtc ggggctctgg gcgcccgccg cgatcctgat tggcgacatc gcgtccaggc
     1621 tgccgcctgg ctccgtgccg ttccagccct gggccgagaa actctacaaa gagcgcctcg
     1681 ccaaccaggg cctcgacgac ccgacggcca actgcatcgt cggcggcgtg ccccggtcgg
     1741 atctcgtccc gtatccgttc aaggtcctgc agcagccggg cctgacggtg atcctgtacg
     1801 aggccgtgca ctcgtaccgg cagatcttca gcgacggccg tccgctgccc acggacccga
     1861 acccggcgtg gttcggctac tccgttggac gatgggagaa ggacaccttc gtcgtcgagt
     1921 cgtcaggctt caacgacagg ggccggctgg acaacaacgg ccacccgatc actgaaaagc
     1981 ttcgagtgac ggagcggttc acgcgcaagg atttcggcca catggacgtc gtcatcacga
     2041 tcgacgatcc gggggcctac accaggccgt gggacgtgac gctcccgctg atactgcggc
     2101 cagacaccga gctgctggag tacatctgca acgagaacaa caagttcttc gagatcattc
     2161 ccaagcagcc ggcaggcagt cggtagcaag cagagaccgt agagcgcagg cttcagcctg
     2221 cgcgccaggg agagaagaga ggcgtagagc gcgggcttca gcctgcgcga gaaggagctg
     2281 gccatgcgta ctgcgatcgt gttcgtcgtc ggtctcgtga ttggatcggc actggctccc
     2341 ggcttcgcgc aggacacgag gattccgggc gtcaacaaca tgaaccacgt ggcgatcgtc
     2401 gtcccgaacc tcgttgacgc cattgcgttc tacacgcaga agatgggatt caaggaaggg
     2461 ttcaccgtca agaacgacca ggggcagccg acgctttcct acattcaggt cagccgcgac
     2521 accttcatcg agctgcagcc cgcgaacgcg aaccgacagc ccggtctgag ccacttcggg
     2581 ctgcacgtcg ccgacatcaa gacgtacgtc gccgagctga agaagcgcgg cgtcacggtg
     2641 acggatccgc gccagggccg aggggactcg atggccgcca acgcgactga tccgggcggc
     2701 atgaacatcg agatcttcca gttcggaccg gactctctcc agggccaggc gatctccagc
     2761 tggaagcagc cgtgaagtcg ctcgccggcg ccgtcgcggt gctggcgggc tcgctctcgc
     2821 tggtgcttgt tgcgcagagc cccgacgttc tggagaacgc cgcgcgatcg atgggcgcgc
     2881 cgggcctcca gtcgattcag tactctggcc gtggatccgt gtacctgctc gggcaggccc
     2941 ccgcgcccgg cgctccctgg cctcgtttcg agctcgcgaa gtacatcgcg tcggtcgatt
     3001 acgcccgcaa cgcgatgcgc gaggagcagg tgcgccgcga cgtggagagg ccgcctcgag
     3061 gaggaggcgc ggggcccttc aaccctgcga cggggcaggg cggcatccgg ccgattccgg
     3121 gcgacgtgtc ccagaacatc gtccgcgacg gacggaccga ggccggcctg attcaaatcg
     3181 cgctgacgcc gcacgggttc ctcaaggcgg ctgtggcgaa caaggcgtcg gtcacgacct
     3241 ccaccgctcg gggacggacg agccacgcga tctcgttcgc catcggccgg catgtcctga
     3301 ccggcacgat cgacgatcgc ggcctcgtcg agcgggtcga gacgcgcatc gccaacgccg
     3361 tgctgggtga catgctggtc gaagcgcttt tcgccgacta caaggatcac ggcggcgtca
     3421 agttcccctc gcgcatcacg catcgccagg gccggcatgc cgtgctcgat ctctcggtgt
     3481 tcgacgtgca gccgaatggc gccgcggcgg cgcctgtcaa cacccccgtt ccgccggcag
     3541 ccgggacggc agcggcgccc ggcggagcgg atgccgaaaa gatcgccgac ggcgtgtggt
     3601 tcctgacggg aggcaacccg atgagcgtcc tcgtcgagtt cagcgaccac gtggtggtca
     3661 tcgaggcgcc tcaaggggag aggcgctcgg aagccacgat tgccgcggtg aagacgctgc
     3721 tgccctcgaa accgattcgg tacgtcgtga acacgcatca tcatttcgac cacgcaggcg
     3781 ggatccgcgg cttcgtggcg gcgggcatcc ccgtcgtcac gcaccaggcg aacaagccgt
     3841 acttcgagcg aatcttcaag aacgcgttca cactcgagcc ggatcgactg gctcgcgcgc
     3901 ctcgccccgc ggtcctcgag accgtggccg acaagagagt gttcgcggat gccgcgatga
     3961 cgctggaatt gca
//
LOCUS       DBNGSH010000496        11839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_726377, whole genome shotgun sequence.
ACCESSION   DBNGSH010000496 DBNGSH010000000
VERSION     DBNGSH010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11839
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_726377"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="AC16CY_11380"
     CDS             <1..1098
                     /locus_tag="AC16CY_11380"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3354438.1"
                     /translation="SLLSSREFAASNFAIDALNHPDPLPFFRTSVGVEQLLYDGGRAR
                     SEASAAALRRDALSHQSDQASADLALAVSQTYGRVLAAQAAERAAAAGVEAAREDLTR
                     AERRRDVGMATDADVLSLRVHLTGLEQRQIEAGGEAAIARAELNRLMGAPLDRAYDIV
                     EPQLEADSRQPIDTAAFVREALASRPSLKSQEAETRLANTLRAESRSGLIPRIAVQGG
                     VDVSGTSFGDRTSAWAVGGELRWTFSTGGAEIARIKSAAEASSRARLEHEDARAAVEV
                     EVVSAVRRLETALARQRVGAAAVDQARESQRIIRDRFEVGMAGTDDVLRASTAVVDAE
                     ARRTAAVVDALVSRAGLERAVGRIPRSRGSL"
     gene            1095..2312
                     /locus_tag="AC16CY_11385"
     CDS             1095..2312
                     /locus_tag="AC16CY_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3354439.1"
                     /translation="MKSLASGRLKLALYVTAASSFVAAAGCRSTATPEPSDSPAAITV
                     STDRIASIDLPARIDAGGIVQAGATAQITSRVIAPIQDVLVRAGDRVTRGQPLVVLDS
                     REVTANAERADATLAAATEASRAAESRIAAAQSTLRLAQATHARVRALHEKRSATAQE
                     LDQAVASLEAAEAQALTARSESAAAASARETARAAATAASVARSYAVLTAPFDGVVAE
                     RSADPGSLATPGSPLLIVEESGPARLDVRLDDSRAAWVRQGQSAAIRLDDDESASWTT
                     TSIVEVGRSDPASHTFLVKLALPPNVTARTGSFGRARFTGESRRTLTAPAASVVRRAG
                     LTFVFVVGADTQARLRPVIVGARDDNRTEVLAGVSDGDVVIVSPPPAVTDGSRVIVGS
                     RAPSPHAGEAHRE"
     gene            2305..5460
                     /locus_tag="AC16CY_11390"
     CDS             2305..5460
                     /locus_tag="AC16CY_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3354440.1"
                     /translation="MSEPRIGVAGRLAAALLHSKLTPLAILASLLLGLYAVVALPREE
                     EPQIVVPMVDVFVDMAGASPAEVEQRVTRPLEKLLWEIPGVEYVYSTSSPGRSLVIVR
                     FLVGQDEDRALVRLHQKLAANAALLPPGASSPVVQARSIDDVPIMALTLWGAAYDDVT
                     LRQMAAQLQETLKEVPNISEIALIGGRPRQISIEIDPPALSARGLDPLAVQQAVSRAN
                     VRSDVLRVVNGNRSTRVDSGGWAESVDDLRRVVVGTIAGGPVHLGDVATVRDGGGEPT
                     SYVVQHPRRGEAYPAVTLSIAKRKGTNATELADMVERKIETVRGYLLPADLHVSVTRN
                     YGETAERKSNELLWHMLLAVVSVSILIWLVLGLRESVVVLTAIPVTLALTLFVFYLYG
                     YTLNRITLFALIFSIGILVDDAIVVVENIVRHARQSGAGRLEDVAVQAVDEVGNPTIL
                     ATLAVIAAILPMAFVGGLMGPYMRPIPVGASAAMVFSLLVAFVVTPWAAVRLLGTSHS
                     PHTDRQEDGLTAMYRRVMNRLITIPSARWRFLGLVAFLLVAAVALIPSGLVTVKMLPF
                     DNKSELQVVVHMPEQTPLETTAGVAEALADAALADPSVESVQSYSGTSSPYTFNGLVR
                     HYFLRRDANMADLQVMLVDPERRDEQSHDIAKRLRTALGPVAARLDATVQVVEVPPGP
                     PVLQTIVGEIYGPDPERRLELARQVRAVFEKTTGVVDVDWYVESDRPRLQMVFDEDKA
                     ASAGVTAADVSAVASMAGEGAPVSLLHDPIARETVPVVMRLPRQQRGSLDAIGSIRLG
                     RPATSVAELTRFGEAREEQSLYHKNLLPVTYVTADVAGAIESPVYAILQMNRVLSGIR
                     FPEGYGLEIFNARQPFDSTKYAMKWDGEWHITYEVFRDLGIAFAAVLVLIYILVVGWF
                     QSFWTPLTIMLAIPFSLVGILPAHALLGAFFTATSMIGFIAGAGIVVRNSIILVDFIE
                     LRIQEGMSLEEAVVDAGAVRFRPMAMTAAAVIVGSAVILFDPIFQGLAISLMAGEVAS
                     LLLSRVTVPIAYYLTHRRLAH"
     gene            5472..5690
                     /locus_tag="AC16CY_11395"
     CDS             5472..5690
                     /locus_tag="AC16CY_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR3354441.1"
                     /translation="MNVERMLRLIAGVFVAASVLLGLYVHPSFLWFTLFVGLNLTQSA
                     FTNWCPMMTILRTSGVPDSTAPRARKAA"
     gene            5919..7112
                     /locus_tag="AC16CY_11400"
     CDS             5919..7112
                     /locus_tag="AC16CY_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354442.1"
                     /translation="MGRLWVGTLAVAIAVSVFALTPVTGQQQQAQAMPRPYGTPPPLV
                     ITAFGGEPITYKAPRTPWGDPDLQGVWSSDDMEGVGIAAGGGRGRGGAPPAAAAAPAA
                     GPPPMYLTGEQLEARRKQVETSASNRDNNSIGTFRNDYARRAFPQTRLLVDPPDGRMP
                     TVLPSARTMPRGTNGVGPLDSWLDFPLYERCITRGIGGSVLRVIYGNGNQIVQGPGVV
                     AFSYEMLPDTRLFHLDGRPHANQAISMYMGDSRARWEGEELVVETTNLTDQTAFGING
                     LGTQHSKAMKITERFRRVADDIIQYQATWDDPLTYSAPFTVSFPLTPLVGNVLLPYEC
                     HPGNKAIEMSLSAERDEDRTMAEAAAKGIKRERRAINQNAGGGGRGGGRGGRGQADTG
                     AEAEK"
     gene            7236..8354
                     /locus_tag="AC16CY_11405"
     CDS             7236..8354
                     /locus_tag="AC16CY_11405"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3354443.1"
                     /translation="MHRRLRVPTSRSAALVILGLTLWAAADVAGQGRRDVARLASEGS
                     AALNERRFGDAHEALSAALALRPQEASLHLGAGVAAFMMGRDGEAQRRFERALEIDPG
                     YQTASLWLGHLHNRAGRLDEAIAVYERALERSPGTPALERPLADWRRSAALQSGLSVV
                     HGAHFSVLFEGPADNALARRVVERLEAAYWRVGAALAAYPTEPVTVVLYSLEQYSDVT
                     RLPQWTAAAYDGRIHLPIGAALQQTEDLDRVLGHEFVHAVVAMLGGRNVPAWLNEGLA
                     TALESEGAEEPGHDLSAASSWPRLSDLHYSFLKFDTDQAHAAYAVSTRAVRRMQELRG
                     AGALVALLRDLSSGVPFASAFHQRMAMRYEDFQTTLTR"
     gene            8465..9562
                     /locus_tag="AC16CY_11410"
     CDS             8465..9562
                     /locus_tag="AC16CY_11410"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR3354444.1"
                     /translation="MPSFAVAPPIEPMLAKLAGELPIGNFLYEPKWDGFRAIVFRAGS
                     DLFIQSRDLRPLDRYFPELHDALLAGLPDDSVVDGEIVIATPRGLDFDALQLRLHPAA
                     SRVAKLAQETPSSFVAFDAIALEGRDLRRAPQEERRALLERALADARPPLHLTPMTRD
                     PAEAKEWLSHFEGAGLDGVVAKAARGTYEPGKRAMIKIKHARTADCVVAGFRWHKAGR
                     NELVGSLLLGLYDSDGRLQHVGVTSAFTMTVRRQLAQELAPLRENALDQHPWCEWAEA
                     GEMARMPGGHSRWSAGKDLSWEPLRVERVCEVKYDHMQGPRFRHAAVFERWRPDKRPL
                     DCRYDQLDVTPPYELAKVFGAARRDSDDRNR"
     gene            9695..9925
                     /locus_tag="AC16CY_11415"
     CDS             9695..9925
                     /locus_tag="AC16CY_11415"
                     /inference="COORDINATES: protein motif:HMM:NF046164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HIR3354445.1"
                     /translation="MPTLSFKTRPAEAKAIRAAARSRGLSVSEYLRRAVLARPVRAKR
                     VHVRFKPGRVVIEGASDAPTLTSADVAVALYE"
     gene            9929..10345
                     /locus_tag="AC16CY_11420"
     CDS             9929..10345
                     /locus_tag="AC16CY_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3354446.1"
                     /translation="MRHLLDNDVFFAAMYEKHVLHKQCRAWLDRAKPGGWAVAAETYL
                     AAVRLLMNPLVLRSGHLTGPEAVEAVEAELAGKHPGRVVIARSRPQADMLKRATGHKQ
                     VMDFWLVQIASEHGCKLATNDAALVAAWPDLVERIT"
     gene            10342..10803
                     /locus_tag="AC16CY_11425"
     CDS             10342..10803
                     /locus_tag="AC16CY_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HIR3354447.1"
                     /translation="MTAPMRYQWSTLNRQQKGTFGEHFAKMEFAMYGFLVFSAEIDDR
                     GIDFVARTDSGKHFDVQVKTITRRNYTYVKKSKFADRLVVCLVVLAEGVGPETYLFRE
                     SDWSKKTDGLLKVNSFPNAKEPEYGINVTKSRQALLEQYRFDRVVATLVKR"
     gene            complement(10832..>11839)
                     /locus_tag="AC16CY_11430"
     CDS             complement(10832..>11839)
                     /locus_tag="AC16CY_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HIR3354448.1"
                     /translation="LELHPHPVRADDLDHPDELRVDLDPVPGVSWDQIREVARVIRVT
                     LAEFALAGWPKTSGSRGMHVNVRIERRWTFDQVRRAALAFAREVERRAPALATSKWWK
                     EERHGVFLDYNQNAKDRTVASAYSVRPTPDARVSAPLTWDEVDECDPRDFTLATMPAR
                     FAAVGDRHDGIDAHPCSLESLLELSARQEREGLGDAPWPPHYRKQSGEPTRVAPSRAR
                     PDGGSPVTRASGRRTPTRPLIEIGRARKKSDALEGLERWKARHPDAAAHLEPADVLVD
                     AMRGRFHTWSRIRVNLQHVPEALRPAQEALDPNDEPDDWGDVMSRGGPHRRPSRARKA
                     E"
BASE COUNT         1881 a         3964 c         4028 g         1966 t
ORIGIN      
        1 tcgcttctgt cgtcgcgcga atttgcggcc tccaacttcg cgatcgacgc cctcaatcac
       61 cccgatccac tccccttctt cagaaccagc gtgggcgtgg agcagctgct gtacgacggc
      121 ggacgggcgc ggtcggaagc ctcggcggcg gcgttgcgcc gtgacgcgct gtcgcaccag
      181 tcggatcagg cgagcgcgga tctcgcactc gccgtgtcgc agacctacgg tcgtgtgctc
      241 gcagcacagg cagccgaacg tgccgccgcg gcgggcgtcg aggccgcccg ggaagacctg
      301 acgcgggccg aacggcgacg ggacgtgggc atggctaccg atgccgacgt actcagcctg
      361 cgcgtgcatc tgaccgggct cgaacagcgt cagatcgagg ctggcggcga ggctgccatc
      421 gcgcgtgccg aactgaatcg cctcatgggc gcacctctcg atcgcgccta cgacatcgtc
      481 gaacctcagc tcgaggccga ctcacgtcag ccgatcgaca ccgctgcctt cgtccgcgaa
      541 gcgctcgcat cgcgcccctc gctcaagagc caggaggcgg aaacacgcct ggccaacacg
      601 ctgcgcgccg agtcacgcag cgggctcatt ccgcggatcg ccgttcaggg aggtgtcgac
      661 gtgagcggca cgagtttcgg ggatcgcacg tcggcgtggg ccgtcggtgg cgaactccgg
      721 tggaccttct cgacaggggg cgccgagatc gcgcgcatca agagcgcggc cgaagcctcg
      781 tcgcgggccc ggctcgagca cgaggatgcc cgggccgcgg tggaggtgga ggtcgtcagc
      841 gcggtgcggc gccttgaaac cgcgctggcg aggcagagag tcggcgcggc tgcggtggat
      901 caggctcgcg aaagccagcg gatcattcgc gaccgattcg aggtcgggat ggcgggaacc
      961 gacgacgtgc tccgcgcgtc gacggccgtc gtcgatgccg aggcgcggcg gaccgccgcg
     1021 gtcgtcgatg ccctcgtcag ccgcgccggg ctcgagagag ccgtcggccg gattccacgt
     1081 tccagaggat cgctgtgaag agtctcgctt cgggccggct gaagctggcg ctctacgtca
     1141 ccgccgcatc gagttttgtg gccgccgcgg gctgccgatc gacggcgacg cccgagccgt
     1201 cggattcccc cgcggccatc accgtctcga cggatcgcat cgcgtcgatt gacctgcccg
     1261 cgcgaatcga tgcgggaggc atcgtgcagg ccggcgcgac ggcccaaatc acgagccgcg
     1321 tgattgcgcc aatccaggac gtgctggttc gggcgggcga ccgcgttaca cgaggccagc
     1381 cgctcgtcgt gctcgacagc cgtgaggtca ccgccaacgc cgagcgggcc gacgccacgc
     1441 tggcggccgc caccgaggcg tcgcgcgccg ccgaatcgcg catcgccgcg gcccagtcca
     1501 cgctccggct ggcgcaggcc actcacgcgc gcgtcagggc actgcacgag aaacgatcgg
     1561 cgacggcgca ggagctcgat caggccgtgg ccagcctcga ggctgccgag gcgcaggcct
     1621 tgaccgctcg ctccgagtcg gcagcagcgg cctccgcgcg cgagacggct cgagccgcgg
     1681 cgaccgcggc gtcggtcgcc agatcgtacg cggtgctgac cgcgcccttc gatggcgtgg
     1741 tcgccgagcg atcggccgac cccgggtctc tcgcgacgcc gggatcgccg ctgttaatcg
     1801 tcgaagagtc cggtccggca cgactcgacg tgcgtctgga cgattccagg gccgcgtggg
     1861 tgcggcaggg tcaatcggca gccatccgcc tcgatgacga cgagagcgcg tcatggacaa
     1921 ccacgtcgat cgtcgaagtg ggtcgatcgg atccggcgtc gcacaccttc ctggtgaagc
     1981 ttgccttgcc gccgaacgtc accgcgcgaa ccggatcctt tggtcgagcg cgcttcacag
     2041 gcgagtcgcg ccggaccctg accgcgcccg cggcgagcgt ggtccggcgc gccgggctca
     2101 ccttcgtctt cgtcgtgggc gcagacacgc aggcacgcct gcgccccgtc atcgttggag
     2161 cacgggacga caaccgcacc gaggttctgg cgggcgtcag cgacggcgac gtggtgatcg
     2221 tgagcccgcc tcccgccgtg accgacggga gtcgcgtcat cgtcgggtcg cgggcacctt
     2281 cccctcacgc cggagaggcg caccgtgagt gaaccccgaa tcggcgtcgc tggacggctg
     2341 gccgcggccc tcctgcattc caagctgacg ccactggcca tcctggcgtc attgctgctc
     2401 ggcctctacg ccgtcgtcgc gcttccccgc gaggaagagc cgcagatcgt cgtgccgatg
     2461 gtcgacgtgt tcgtggacat ggccggcgcc tctccagcgg aggtcgagca acgcgtgacg
     2521 cggccgctcg agaagctcct gtgggagatc ccgggtgtcg agtacgtcta ctcgacgtcg
     2581 agccccggcc gctcgctcgt gatcgtccgg ttcctcgtgg gccaggacga ggatcgcgcg
     2641 ctggtgcgcc tgcatcagaa gctcgccgcc aacgcggcgc tgcttccgcc cggcgcgtca
     2701 tcaccggtcg tgcaggcgcg ctcaatcgac gacgtgccga tcatggcgct cacgctgtgg
     2761 ggcgccgcct acgacgatgt gaccctcagg cagatggcgg cgcagctcca ggagacgttg
     2821 aaggaagtcc cgaacatctc cgagatcgcg ttgatcggag gacgtccgag gcagatctcg
     2881 atcgagatcg atccgcccgc actctcggcg cgaggcctcg acccgctcgc cgtgcagcag
     2941 gcggtgtcgc gggccaacgt cagaagcgac gtgctgcgcg tggtcaacgg gaaccggtcg
     3001 acccgggtcg acagcggggg ctgggccgag tcggtggacg atctccggcg cgtcgtggtg
     3061 gggacaatcg ccggcgggcc ggttcacctg ggcgacgtgg ccacggtgcg tgacggcggc
     3121 ggcgaaccca cctcctacgt ggtgcagcat ccgcgacgcg gcgaggccta tccggccgtc
     3181 acgctgtcga ttgccaagcg caaagggacc aacgcgacgg agctggccga catggtggaa
     3241 cggaagatcg agaccgtgcg cggctacctc ctgccagcgg atctgcacgt gtccgtcacg
     3301 cgcaactacg gcgagacggc cgagcgcaag tcaaacgagc tgctgtggca catgctcctc
     3361 gccgtcgtgt cggtgtcgat cctcatctgg ctcgtcctcg gactgcggga gtccgtggtg
     3421 gtgttgacgg cgatccccgt cacactggcg ttgaccctct tcgtcttcta cctctacggc
     3481 tacacgctca accgcatcac gctgttcgcg ctcatcttct ccatcgggat cctcgttgac
     3541 gatgcgatcg tggtcgtgga gaacatcgtc cggcacgcgc ggcagtcggg cgccggcagg
     3601 ctcgaagacg tcgccgtgca ggcggtggac gaggtcggca acccgacgat cctggcgacg
     3661 ctggccgtga tcgcggccat cctgccaatg gcgttcgtcg gcggcctgat gggtccctac
     3721 atgcggccga ttccggtcgg cgcctcggcg gcgatggtgt tctcgctgct ggtcgcgttc
     3781 gtcgtgacgc cctgggcggc ggtacgtctg cttggcacgt cacactcgcc gcacacagac
     3841 aggcaggagg acgggctcac ggcgatgtac aggcgcgtga tgaaccgtct catcacgatc
     3901 ccttcggcgc ggtggcggtt cctcgggctg gtcgccttcc tgctcgtggc cgccgtggcg
     3961 ctgattccgt caggcctcgt cacggtgaag atgctcccct tcgacaacaa gagcgagctg
     4021 caggtcgtgg tccacatgcc ggagcagaca ccgctcgaga cgacggcagg cgttgccgag
     4081 gcgctcgccg atgccgcgct ggccgatccg tcggtcgaga gcgtgcagag ctattcgggc
     4141 acgtcgtcgc cctacacgtt caatggactg gtgcggcact acttcctgag acgcgacgcc
     4201 aacatggccg acctccaggt gatgctcgtc gacccggagc ggcgcgacga gcagagtcac
     4261 gacatcgcca agcgcctgcg cacggcgctc ggacctgtgg cggcacgtct ggacgctact
     4321 gtgcaggtag tcgaggtccc gccgggcccg cccgtgctcc agacgatcgt cggtgagatc
     4381 tacggccctg accccgagcg ccggctggag ctcgctcgtc aggtgcgagc cgtcttcgag
     4441 aagaccacag gcgtggtgga cgtcgactgg tacgtcgagt cagaccgacc caggctgcag
     4501 atggtcttcg acgaggacaa ggccgcctcc gccggcgtca cggcggcaga cgtgtcggcg
     4561 gtcgcgagca tggcaggcga gggagcgccg gtcagcctcc tgcacgaccc gatcgcgcgc
     4621 gagaccgtcc ccgtcgtgat gcgtcttccg aggcagcagc gcgggtccct cgacgccatc
     4681 gggagcatcc gccttgggcg cccggccacc tccgtcgccg agcttacgcg attcggagaa
     4741 gcacgtgagg agcagagcct gtatcacaag aacctcctcc cggtgaccta cgtgacggcc
     4801 gatgtggctg gcgcgatcga gagccccgtg tacgcgatcc tccagatgaa ccgggtcctc
     4861 tcgggcattc ggtttcccga aggctacggt ctcgagatct tcaatgcgcg tcagccgttc
     4921 gacagcacga agtacgcaat gaagtgggac ggcgagtggc acatcaccta cgaggtgttc
     4981 cgcgatctcg ggatcgcgtt tgctgccgtc ctcgtgctca tctacatcct cgtggtcggc
     5041 tggttccagt cgttctggac gccgctgacc atcatgctgg cgatcccctt ctcgctcgtc
     5101 ggcattctgc ccgcgcacgc gctgctcggt gccttcttca cggcgacgtc gatgattggt
     5161 ttcatcgccg gcgcggggat cgtggttcgg aactcgatca tcctcgtgga cttcatcgag
     5221 ctgcggatcc aggaggggat gtcgctcgaa gaggcggtcg tcgatgccgg cgccgtccgt
     5281 ttccgcccga tggcgatgac ggcggcggcg gtcatcgtcg ggtcggcggt gattctgttc
     5341 gacccgatct ttcaagggct ggctatctcg ctgatggccg gtgaggtggc ctcgcttctg
     5401 ctttcacgtg tgaccgtgcc gattgcgtac tacctcacgc atcgccggct tgctcattga
     5461 ggagaacgga catgaacgtc gaacgcatgc ttcgcctgat tgccggagtg ttcgtagcag
     5521 ccagcgtgct gctcggcctc tacgtgcatc cgtccttttt gtggttcacg ctgttcgtcg
     5581 gtctgaacct gacacagtcg gccttcacca actggtgccc gatgatgacg atcctgcgca
     5641 cgagcggcgt accggacagc acggctccgc gcgcaaggaa agcagcgtag cgcagacccg
     5701 gtgctggatg ggcccgtcat gcgaccatgg cgccttgcga tgtcgatcga cgccgcctgg
     5761 gccattgcaa gcccggcgcg tcgactggat ctcgggaact tgggtcgtgc aaccgatagc
     5821 gaccgtcctt gaacaacgcc gcgcaggagc ctagaattcc cgacagtttc tgccgctctc
     5881 attcacgctg ccttcgcagc aggcgcaagg agttcatcat gggtaggctc tgggttggca
     5941 cgctcgcggt ggcgatcgcc gtgtcggtct tcgcccttac tccggtcacg gggcagcagc
     6001 aacaggctca ggcaatgccg aggccgtacg gcacgccgcc accgctcgtc atcaccgcct
     6061 tcggcggaga gccgattacc tacaaggcgc cgcgcacgcc gtggggcgac ccggatctgc
     6121 agggggtatg gtccagcgac gacatggaag gtgtcggcat cgccgccggc gggggaaggg
     6181 gtcgaggcgg cgctcctccc gctgctgcag ccgcgccggc tgccggtccc ccgccgatgt
     6241 atctgacggg cgaacagctc gaggcgcgga gaaagcaggt ggagacctcc gcctcaaacc
     6301 gcgacaacaa ctccatcggc acgttccgta acgactacgc gcggcgcgcc ttcccgcaga
     6361 cgcgcctgct ggtcgatcca ccggacggcc gcatgcccac cgtgcttccg agtgcccgga
     6421 cgatgccgcg cggcacgaac ggcgtcggcc cgctcgactc gtggctggac ttcccgctct
     6481 atgagcgctg catcacgcgc ggcatcggcg gctcagtcct ccgcgtgatc tacggcaacg
     6541 gcaaccagat cgtgcaggga ccgggcgtgg tggcgttctc ctacgagatg ctcccggaca
     6601 cgcgcctctt ccacctggat ggccgtccgc atgcgaacca ggcgatctcg atgtacatgg
     6661 gcgactcacg cgcgcgctgg gaaggcgaag agctcgtcgt ggagacgaca aacctcacgg
     6721 accagaccgc gttcggcatc aacggcctcg gaacgcagca cagcaaggcg atgaagatca
     6781 ccgagcgttt ccgccgcgtt gccgacgaca tcattcagta ccaggcaacg tgggacgatc
     6841 cgctcaccta ctcggcgccg ttcaccgtct cgttcccgct gacgccgctc gtcggcaacg
     6901 tgctgctgcc gtatgaatgc catccaggca acaaggcgat cgagatgtcg ctctcggcag
     6961 aacgcgacga ggatcggacg atggcggaag cggccgccaa ggggatcaag cgcgaacgcc
     7021 gggccatcaa ccagaacgcc ggcggcggtg gacgcggcgg cggccggggg ggacgcggcc
     7081 aggcggatac cggcgcagaa gcggaaaagt aataggcccc ggaagatccg atccaggcgg
     7141 cgttttgtct gcgcgcgaag ggacttcgga cgaccctgac tcgcccgatc tcactgtcct
     7201 gttggggttt cgcagatcag gaccgggggc cgatcttgca ccggcggctg cgcgtgccca
     7261 ccagcaggtc ggcggcgttg gtcatcctcg ggctcaccct gtgggccgcc gcggacgttg
     7321 ccggccaggg cagacgtgac gtcgcaaggc tcgcctccga gggttcagcc gcgctcaacg
     7381 agcgccggtt cggcgacgcg cacgaagcct tgagcgcggc gctggccttg cggccgcagg
     7441 aagccagcct gcatctcggc gccggcgtgg cggcattcat gatggggcgc gacggggagg
     7501 cgcaacgccg tttcgaacgc gccctcgaga tcgatcccgg ttatcagacc gcgtccctct
     7561 ggctgggaca cctgcacaat cgagccggtc gtctggacga ggccatcgct gtgtacgagc
     7621 gggcactcga gcgctcgccg ggaacgccgg cgctcgaacg gcccctcgcc gactggcgcc
     7681 gctcggccgc gttgcagagc ggtttgtccg tggtgcatgg ggcgcatttc agcgtgctgt
     7741 tcgagggacc ggccgacaac gccctcgcgc gccgcgtcgt cgagcgtctc gaggcggcct
     7801 attggcgagt gggcgccgcg ttggccgcct atccaaccga gcctgtaacc gtcgtcctgt
     7861 actccctgga acagtacagc gacgtcacga ggctgccgca atggactgct gccgcctacg
     7921 acgggcgtat ccatctgccc atcggcgcgg cgttgcagca gaccgaggac ctggaccggg
     7981 tcctcggaca cgagttcgtc cacgccgtgg tcgcaatgct cggcggccgc aatgtcccgg
     8041 cgtggctgaa cgaagggctc gctacggcgc tcgaaagcga aggcgcggag gagcccgggc
     8101 acgatctgtc ggcggcgagc tcgtggccgc ggctgagcga tctccactac agcttcttga
     8161 aattcgacac ggaccaggcg cacgccgcct acgctgtcag cacacgggcc gttcgccgga
     8221 tgcaggaatt acgcggggca ggggccctgg tcgcgcttct ccgcgatctg tccagcggcg
     8281 tacccttcgc ctccgcgttt caccagcgca tggcgatgcg gtacgaggac tttcagacga
     8341 cgctcacgcg ctagtcggtc ctttgtcctt ggttcctagt gcttggtccg tcctgtgggt
     8401 ccttggtccg tccttggtcc ttggtcctcg gtccgccgaa ctatagttga cggctagact
     8461 cttcatgccg tcgttcgcgg tcgcgccccc gatcgagccg atgctcgcca agcttgccgg
     8521 cgagctgccg atcggtaact tcctgtacga gccgaagtgg gacggctttc gcgcgatcgt
     8581 ctttcgcgcg gggagcgacc tcttcatcca gagccgcgat ctgcgcccgc tcgatcgata
     8641 ctttcccgag ctgcacgacg cgctgctcgc cggcctgccc gacgactcgg tcgtggacgg
     8701 cgagatcgtc atcgccacgc cgcgcggcct cgacttcgac gccctccagc tccgccttca
     8761 ccccgcggct tcccgagtcg cgaagctggc tcaggagacg ccgtcgtcat tcgtggcctt
     8821 cgatgcgatc gcactcgagg ggcgcgatct gcggcgggcg ccacaggagg aacggcgcgc
     8881 gctgctggag cgtgcccttg ccgatgccag accgccgctg cacctgacgc cgatgacgcg
     8941 cgaccccgcg gaagcgaaag aatggctgtc ccatttcgag ggggccggcc tcgacggcgt
     9001 ggtcgccaag gccgcgcgcg ggacgtacga gccgggcaag cgcgcgatga tcaagatcaa
     9061 gcacgcgcga acggctgact gcgtcgtggc cggcttccgg tggcacaagg ccgggcggaa
     9121 cgagctcgtc ggatcgcttc tgctcgggct gtacgactct gacgggagat tgcagcacgt
     9181 cggcgtgacg tcggcgttca cgatgacggt ccgccgccaa ctggcacagg agctcgcgcc
     9241 actgcgcgag aacgcgctcg accagcatcc gtggtgcgag tgggcggaag cgggcgagat
     9301 ggcccgcatg ccgggcggcc acagccgctg gagcgcaggc aaagacctgt cgtgggaacc
     9361 gcttcgcgtc gagcgggtgt gcgaagtgaa atacgaccac atgcagggac cgcgattccg
     9421 gcatgcagcg gtgttcgagc ggtggcggcc cgacaagcgt cccctcgatt gccgctacga
     9481 tcagctcgac gtcactccgc cttacgagct cgcgaaggtc ttcggcgcgg cccgccgcga
     9541 ctcagacgat cgcaaccgtt agagagccgt tccgcagagg tcgcggaggg gcccgtacga
     9601 cccgcgcggg gcacggcgat cgccgactac tcgggtcgac actactcgag caggtcactt
     9661 gacgatggtg agttgtggta ataccgtatt atccatgccg acgttgtcct tcaagacgcg
     9721 gcccgccgaa gctaaggcga tccgggcggc cgcccgcagc cgcgggttga gcgtatctga
     9781 gtacctgcgc cgtgcggtgc tcgcccgtcc cgtgcgcgcg aaaagagtgc acgtgcggtt
     9841 caagcccggg cgcgtcgtca tcgagggcgc gagcgacgcg ccaaccctga cttcggcgga
     9901 tgtggccgtc gcgctctacg agtagcgcgt gcgccacctg ctcgacaacg atgtcttctt
     9961 cgcagctatg tacgagaagc acgtactcca caagcaatgc cgtgcatggc tcgatcgggc
    10021 gaagccggga ggctgggcgg tggcggctga aacatacctg gcggccgtgc gactcttgat
    10081 gaatccgctg gtgctcagat cgggtcatct caccggtcct gaggcggtcg aggcggtcga
    10141 ggccgagttg gccgggaaac atccgggacg cgtcgtcatc gcgaggagca ggccgcaggc
    10201 agacatgctc aagcgcgcaa ccggtcacaa acaggtcatg gatttctggc ttgttcaaat
    10261 cgcatccgaa cacggttgca aactggccac gaatgacgcc gcgctggtag cggcctggcc
    10321 ggatctggtt gagcgaatca cttgacggca ccgatgcggt atcaatggtc gacgctgaat
    10381 cgtcagcaaa agggcacatt cggcgaacat ttcgcaaaga tggaattcgc gatgtacgga
    10441 ttcctcgtct tctcggccga gatcgacgac cgtggcatcg acttcgtcgc acgaactgat
    10501 tccggcaaac actttgacgt ccaggtgaag actattaccc ggcgaaacta cacgtacgtg
    10561 aagaagagca agttcgctga ccgcctcgtg gtctgcctcg ttgttctcgc agaaggtgtg
    10621 ggccctgaga cgtatctgtt tcgcgagtct gattggagta agaagacgga cggattactg
    10681 aaagtgaatt cgttcccgaa cgcgaaggag cctgaatacg gcatcaacgt gaccaagtcg
    10741 cgtcaggccc tattggagca ataccgattt gaccgcgttg tagccaccct cgtgaaacgc
    10801 tagcctgcca ctaaacatgc tgccgaggcg gtcactccgc cttacgagct cgcgaaggtc
    10861 ttcgatgcgg cccgccgcga ctcatgacgt cgccccaatc gtccggctcg tcgttggggt
    10921 cgagcgcctc ctgggccggc cgcagggcct cgggcacgtg ctggaggttc acgcgaatcc
    10981 gcgaccacgt atgaaaccgc ccgcgcattg cgtcgaccag cacgtcggcg ggctcgaggt
    11041 gggcggccgc gtcgggatga cgcgccttcc agcgctcgag cccctcgagg gcgtcgctct
    11101 tcttccgggc gcggccgatc tcgatgagcg gccgcgtcgg cgtccttcgg cccgacgccc
    11161 gggtaaccgg gctcccgccg tccggacgtg cgcggctcgg cgcgacccgc gtgggctcgc
    11221 cggactgctt gcggtagtgc ggcggccagg gcgcgtcgcc aaggccctct cgctcctggc
    11281 gtgccgacag ctccagcagg ctctcgagcg agcatggatg cgcgtcgatt ccgtcgtgcc
    11341 gatccccgac ggccgcgaag cgcgcgggca tcgtggcgag ggtaaagtcg cggggatcgc
    11401 actcgtccac ctcgtcccac gtcaagggcg cagagacgcg cgcatcaggt gtcggacgaa
    11461 cggagtacgc gctcgcgacg gtgcggtcct tcgcgttctg gttgtagtcg aggaagacgc
    11521 cgtggcgctc ctccttccac cacttgctgg tggccagcgc aggcgcccgt cgctcgacct
    11581 cgcgcgcgaa cgcgagcgcg gcgcggcgca cctggtcgaa cgtccagcgc cgctcgatcc
    11641 gcacgttgac gtgcatgccg cgcgatccgg acgtcttcgg ccagccggcg agcgcgaact
    11701 cggcgagagt cacccgaatc acgcgcgcga cctcgcgtat ctgatcccag ctcacgccag
    11761 gtacggggtc gaggtcgacc ctcagctcgt cgggatgatc gagatcatcc gcgcgaacag
    11821 ggtgcgggtg gagctcgag
//
LOCUS       DBNGSH010000497         3887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_216667, whole genome shotgun sequence.
ACCESSION   DBNGSH010000497 DBNGSH010000000
VERSION     DBNGSH010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_216667"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="AC16CY_11435"
     CDS             <1..161
                     /locus_tag="AC16CY_11435"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3354449.1"
                     /translation="AVESLDGMTADWARLPHELLAAISSRIVNEVRGINRVVYDISSK
                     PPSTIEWE"
     gene            307..3882
                     /locus_tag="AC16CY_11440"
     CDS             307..3882
                     /locus_tag="AC16CY_11440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3354450.1"
                     /translation="MSEFVHLHLHTEFSLLDGACRIDELLDEAVKLKMPALAVTEHGN
                     MFSSVAFHDHARERGLKPILGCEVYVAQGSRFDKSGPQTETNHLVLLAETDQGYRNLI
                     KLVSAGYTEGFYYRPRIDKDLLAQYSTGLIGLSSCLKGEVASALKVEQARQALDAAGR
                     LRDILGKDNFFLEMQYQGIEEQKVVNRGIVPLARELGLKLVATNDVHYLRQGDYQPHD
                     ILLCIGTGKTVNDAQRLRYTGDQFFLKTADQMAAVFGDYPEALRNTLLISERCNVTIP
                     QGQSHLPSFGVPAPFTLDEYFEHVAREGFAQRLPRLQQLSAAGRLQHPLEEYESRLEY
                     EIAMIRKMGYSGYLLIVWDFIRYAREAGIPVGPGRGSAAGSLVAWCMRITDVDPIDFD
                     LIFERFLNPERVSLPDIDVDFCERRRGEVIEYVTRKYGRENVAQIITFGTMKAKAVVR
                     DVGRALDMPYADVDRIAKQIPPALDMTLEKALAENPALTEMASRDARVKDVIEIGKRL
                     EGLSRHASVHAAGVVIAPGPITDYAPLYKGARDEITTQWNMKEVERIGLLKMDFLGLS
                     TLTLIHDCLEEIKRTEGADLDIDAIPLDDPKTYRVFGDGAAFGIFQFESSGMRDLLRK
                     AKPSRLDDLIALNALYRPGPLKSGMVDDWVARKQGKTEVKYELPQLEPILSGTYGVIA
                     YQEQVMRIAQALAGFSLGQADVLRKAMGKKDPKVMAMQREAFMEGARAKGVNEKKATK
                     IFDLMEYFAGYGFNKSHSTAYALLAYQTAYLKANYPWHFAAALLTIEAQNTDKLALYL
                     AESRERGVPVLPPDINESQLAFSVEPGKGVRFGLTAIKGLGEGAIRAIIDARTALGGR
                     IPSLPALCEILDLRIANKRVFEALVKSGACDSLAAEGPLTSGTPRNAIRARLFASVDA
                     ACEHGSRLQRNKDLGQVDLFGGGDGAGPTIAPLPDAAPWTEIEQLNFEKEALGLYWSG
                     HPIDRHAALLREYGAKTTLDLVPRRETVDAGEENGVEPAARAENGNGGGRAAEDVAIG
                     GIVFGLRALKTRKGDRMCVFSLEDATGSIEVVVFPDAFKQHGHVVENGRSVLVHGRYE
                     RDEDSIRVLASEIVPIEMVQERLARLVAIRVSMPPHGRATFERLLELLAQHKGDRRVA
                     FVILEQDRHLRVTADVSGIKVRPSERLVSEVEKICGAGSVNLRSS"
BASE COUNT          734 a         1205 c         1311 g          637 t
ORIGIN      
        1 gggcagtcga aagccttgac ggcatgacgg ctgactgggc gcgcctgcct cacgaactgc
       61 tcgcggccat ctcgtcacgg atcgtcaacg aggttcgtgg tatcaaccgg gtggtgtacg
      121 acatctcatc gaagccaccg tcgacgattg agtgggaata gcggagatca ttgttgattc
      181 atggatcttt gattgttgat tcgttgtcga ttgaagatcc gcgattgttg attggaacca
      241 cgacaatcac gaatcaacaa tcgacagtca ccaacgaatc gacaatcacc aatcaaagat
      301 cagtagatgt ctgaattcgt ccacctccac ctgcacacgg agttctcgct gctcgacggc
      361 gcctgccgga tcgacgagct gctggacgag gccgtgaagc tgaagatgcc cgcgctggct
      421 gtgaccgagc acgggaacat gttctcgtcg gtggcgtttc acgaccacgc gcgggagcgg
      481 gggttgaagc cgatcctggg ctgcgaggtg tacgtcgcac aggggagccg cttcgacaag
      541 agcggcccgc agaccgaaac gaaccacctc gtcctgctgg cggagacgga ccagggatac
      601 cgaaacctga tcaagctggt ctcggcgggc tataccgagg ggttctacta tcggccacgc
      661 atcgacaagg atctgctcgc gcagtactcg accggactca tcggactgag cagctgcctg
      721 aagggcgagg tcgccagcgc actcaaggtg gagcaggcac gccaggcact cgacgccgcc
      781 gggcggctgc gggacattct ggggaaggac aacttcttcc tcgagatgca gtaccaggga
      841 atcgaggaac agaaggtcgt caaccggggg attgtgccgc tggcgcgtga actcggcctg
      901 aagctcgtcg cgaccaacga cgtgcactac ctgcgtcagg gggattacca gccgcacgac
      961 atcctgctgt gcatcggcac gggcaagacg gtcaacgacg cgcagcgcct ccgctatacc
     1021 ggcgatcagt tcttcctgaa gaccgccgat cagatggcag ccgtgttcgg cgactatccg
     1081 gaggcgctgc ggaacacgct cctcatcagc gagcgctgca acgtcaccat tccgcagggg
     1141 cagagccacc tcccgtcgtt cggcgtgccg gccccgttca cgctcgacga gtacttcgag
     1201 cacgtcgcgc gggaagggtt cgcccagcgc ctcccgcgcc tgcagcagct ctcggcggcg
     1261 ggccggcttc agcacccgct cgaggagtac gagagccgcc tcgagtacga aatcgcgatg
     1321 atccgaaaga tgggctactc ggggtacctg ctcatcgtgt gggacttcat tcgctacgcg
     1381 cgggaggcgg gaattccggt cggcccgggc cgtggatcgg cggcaggttc gctggtggcc
     1441 tggtgcatgc gcatcaccga cgtcgatccc atcgatttcg acctcatctt cgagcgcttc
     1501 ctgaaccccg agcgcgtgtc gctgcccgac atcgacgtgg acttctgcga gcggcggcgg
     1561 ggcgaggtga tcgagtacgt cacccggaag tacggccgcg agaacgtcgc ccagatcatc
     1621 acgttcggga cgatgaaggc gaaggccgtc gtgcgggacg tggggcgcgc gctcgacatg
     1681 ccgtatgcgg acgtagaccg gattgccaag caaatccccc cggcgctgga catgaccctc
     1741 gagaaggcgc tcgcggagaa ccccgcgctg acggagatgg cttcccggga cgccagggtc
     1801 aaggacgtga tcgagatcgg caagcgtctc gagggcctgt cgcggcacgc gtcggtgcac
     1861 gccgccggcg tggtcatcgc gccagggccg attaccgact acgcgcctct ctacaagggg
     1921 gcgagggacg aaatcaccac gcagtggaac atgaaagagg tcgagcgcat cggcctcctg
     1981 aagatggact tcctcgggtt gagcacgttg acgctcatcc acgactgcct cgaggagatc
     2041 aaacgtaccg agggggccga cctcgacatc gacgccatcc cgctcgacga cccgaagacg
     2101 tacagggtct tcggcgacgg cgcggcgttc ggcatcttcc agttcgagag ctcggggatg
     2161 cgcgacctgc ttcgcaaggc caagcccagc cggctcgacg acctcattgc gctcaacgcg
     2221 ctctaccggc cgggtccgct caagtcggga atggtggacg actgggtcgc gcgcaagcag
     2281 gggaagaccg aggtcaagta cgagcttccg cagctcgagc cgatcctctc gggcacctac
     2341 ggcgtgatcg cctaccagga acaggtcatg cgcatcgcgc aggcgctcgc cggcttcagc
     2401 ctgggacagg ctgacgtgct tcgcaaggcc atggggaaga aggatcccaa ggtcatggcc
     2461 atgcagcgcg aggcgttcat ggaaggggcc cgcgccaagg gcgtcaacga gaagaaggcc
     2521 accaagatct tcgatctgat ggaatacttc gccgggtacg gcttcaacaa gtcgcactcc
     2581 acggcgtacg cgctcctggc gtaccagacg gcctacctga aagcgaatta cccctggcac
     2641 ttcgccgcag cgctcctgac gatcgaggcg cagaacaccg acaagctggc gctgtacctc
     2701 gccgagagcc gggagcgcgg cgtcccggtg ctgccgcctg acatcaacga aagtcagctg
     2761 gcgttctccg tcgagccggg gaagggagtt cgcttcgggc tgacggcgat caagggcctt
     2821 ggcgaaggcg ccatcagggc catcatcgac gcgcggacgg cgctgggcgg acgcatcccg
     2881 tcgctgcctg cgctgtgcga gatactcgac ctgcgcatcg cgaacaagcg ggtgttcgag
     2941 gcgctggtga agtcgggtgc ctgcgattcg ctggcggccg agggccccct gacgtccggg
     3001 acgcccagaa acgccatccg ggcgcggctc ttcgcctcgg tcgacgccgc ctgcgagcac
     3061 ggcagccgcc tccagcgcaa caaggatctc ggccaggtcg atctgttcgg tggaggcgac
     3121 ggcgccggtc cgacgatcgc gccgctgccc gatgcggcgc cgtggacgga aatcgagcag
     3181 ttgaatttcg agaaggaagc ccttggactg tactggagcg gtcatccgat cgatcggcat
     3241 gccgccctgc tgcgcgagta cggcgccaag accactctcg atctcgtgcc ccgccgggaa
     3301 accgttgacg caggcgagga gaatggcgtc gagccggcgg ccagggcaga gaatgggaat
     3361 ggaggcgggc gggccgccga ggacgtcgct attggcggca tcgtcttcgg gctgcgggcg
     3421 ctgaagacac ggaaaggcga ccgtatgtgc gtattctctc tcgaggatgc gaccgggtcg
     3481 atcgaagtcg tggtgttccc ggacgccttc aagcagcacg gccacgtcgt cgagaacggc
     3541 cgaagcgtgc tcgtgcacgg gaggtacgag cgcgacgagg actcgattcg tgtcctggcg
     3601 tcggagattg tcccgatcga gatggtccag gagcgcctgg cccgtttggt ggccatccgg
     3661 gtgtccatgc cgcctcatgg ccgtgcgacg ttcgagcgcc tgctggagct gcttgcccag
     3721 cacaagggcg accggcgcgt ggcgttcgtg atcctggagc aggatcgcca cctgcgcgtc
     3781 acggccgacg tcagcggcat caaggtccgt ccttcggagc gcctcgtgtc ggaggtcgaa
     3841 aagatctgcg gggcagggtc ggtgaacctg cggtcgtcgt gagcgaa
//
LOCUS       DBNGSH010000498         2846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1234798, whole genome shotgun sequence.
ACCESSION   DBNGSH010000498 DBNGSH010000000
VERSION     DBNGSH010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1234798"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..554
                     /locus_tag="AC16CY_11445"
     CDS             42..554
                     /locus_tag="AC16CY_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354451.1"
                     /translation="MGTQGIQIAVLATAMTIGGPVLGLAQSSQTKGDTYTWHGELVSS
                     DTTARTMTVKARVAYHEAISELKHFKAGDRVWIVWSGVHDSSDAVRQVRRSDTGRTID
                     ENLVLPAELVSTEAQNQYLTIRVKVPESGLAAIKTVKPGEWVTVTSRHRPSTEDDAVV
                     AIRPYGATTR"
     gene            564..1196
                     /locus_tag="AC16CY_11450"
     CDS             564..1196
                     /locus_tag="AC16CY_11450"
                     /inference="COORDINATES: protein motif:HMM:NF016825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR3354452.1"
                     /translation="MSPPGPGDISLRDFIEAVGSGEAAHGAVSTAAVAGGLGSSLLIM
                     VATLPHSRSDSVADRAKLVEAAAALTGLRQQLIETIETETAVKLFIARNMPQTSAAQR
                     SERHAAIQLALRASADVPLEVMRLCARGAELGATVAAHGARAASADVQLGAALLQAGF
                     GGARSNLEGKLSSFIDAAYITSIVDEIGRLTGEIAASTRAGESLLKAPPA"
     gene            1293..2369
                     /locus_tag="AC16CY_11455"
     CDS             1293..2369
                     /locus_tag="AC16CY_11455"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3354453.1"
                     /translation="MTAALALGGWAVAAQRAAAKATPRTVTARGPLLPGEQSTVDLFE
                     KARASVVYISTQARVMDAWTRNVFSVPRGTGSGFIWDSNGHIITNRHVVAGATGGARV
                     RLSDGRDVEATVVGVSAAHDLAVLKVNLASLPPPLPLGSNSDLRVGQSTFAIGNPFGL
                     DWTLTTGIVSALDRSLPTEDGRGLIEHLIQTDAAINPGNSGGPLLDSAGRLIGVNTVI
                     FSPSGASAGVGFAIPADTVNRVVPQLIASGRYLRPALGIEIDDGLNQLISQRLGVAGV
                     AILRTSPGSAAATAGLRGLRTERDGAILPGDIIVAVNGQAIDSVARLQSRLDDYNVGD
                     TVRLTVLRDKQRVDVPVRLQASTQ"
     gene            2373..2537
                     /locus_tag="AC16CY_11460"
     CDS             2373..2537
                     /locus_tag="AC16CY_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354454.1"
                     /translation="MRCARLVRECCRRGLLVLGAGQNAIRLLPPLVLTASQADTAIGI
                     LDQALAAIRL"
     gene            2582..>2846
                     /locus_tag="AC16CY_11465"
     CDS             2582..>2846
                     /locus_tag="AC16CY_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354455.1"
                     /translation="MRSIVVALVIVTGVTAMAHAETRTVTFNQDPVNQPPKGFEFGLT
                     AAVGKPGAWVVQAEGSNRFLAQTAPDSTRSRFPLAVLSDVTAGD"
BASE COUNT          489 a          872 c          986 g          499 t
ORIGIN      
        1 caggcacgcg taggaggcgc gacaagcaaa aggaggaacg tatgggcaca caggggattc
       61 agatcgccgt cttggcaacc gccatgacga tcggcgggcc ggtgctcggc ctcgcgcaat
      121 cgtcgcagac gaagggcgac acgtatacat ggcatggcga actggtgtcg agtgacacga
      181 ccgcgaggac catgacggtg aaagcccgcg tcgcgtatca cgaggcgatt tcggagctga
      241 agcacttcaa ggccggcgac cgagtgtgga ttgtctggtc cggggttcat gactccagcg
      301 acgctgtgcg acaggttcga cggtccgaca ccggccgcac gattgacgag aacctcgtgc
      361 tccctgccga gctggtatcg acggaggcgc agaatcagta cctcacgatt cgcgtcaagg
      421 tgccagagag cgggttggcg gcaatcaaga ccgtgaagcc cggggagtgg gtcaccgtga
      481 cgtcccgaca ccggccctcg accgaggatg acgccgtggt cgcgatcagg ccgtacggcg
      541 cgaccacgag gtaaggtatg gccatgagtc cgccaggtcc gggcgacatt tcattgcgag
      601 acttcatcga ggctgtcggt tccggcgagg cggctcacgg cgcagtgtcg acagccgccg
      661 tggcaggagg cttagggagt tcgcttctca taatggtcgc gacgctgcct cattcgagat
      721 cagactcggt tgccgatcgg gcgaagctgg tggaagcggc ggctgcgttg accggtctgc
      781 gacagcaatt gattgaaacg atcgaaacgg aaactgcagt aaagctcttc atcgcgcgca
      841 acatgccgca aacgagcgcg gcgcaacgct cggaacggca cgcggcgata cagctcgcgc
      901 ttcgtgcctc tgcagacgtg cctctcgagg tgatgcgctt gtgtgcgcgg ggagcggagc
      961 tcggggccac agtcgcggcg catggtgctc gcgcggcatc ggccgacgtt caactcgggg
     1021 cggcgctctt gcaggcgggc ttcggcggcg cgcgttccaa tctcgagggc aaactgagca
     1081 gcttcatcga cgcggcgtac atcacatcga tcgtggacga gatcggtcgc ctgactggtg
     1141 agattgcagc ttcgacgcgt gccggagagt cgttgttgaa ggcaccgcca gcctaaactg
     1201 tttcgtgagg taggggactt gacgtgcgtt cgtcattact gaagacaacg ggtttctcgt
     1261 atccctcgcg tgcccaactc gcggtcgccg gattgactgc ggcgcttgcg cttggaggct
     1321 gggcggtcgc ggcgcagcgt gcggcggcca aggcgactcc acggaccgtg acggctcgag
     1381 gtccgctgct gcccggtgag cagtcgacgg tcgatctgtt cgagaaggcg cgcgcatcgg
     1441 tcgtctacat cagcacgcag gcgcgggtga tggatgcgtg gacgcgcaac gtcttcagcg
     1501 tcccgcgcgg taccggctca ggattcatct gggactcgaa cggacacatc atcaccaacc
     1561 gccacgtcgt cgccggtgcg acgggcggtg cgcgtgttcg cttgagcgac ggccgcgacg
     1621 tcgaggcgac ggtcgtcggc gtgagcgccg ctcacgacct cgcggtgctg aaagtgaacc
     1681 tcgccagcct gccgccgcca ttgccgctgg gcagcaactc ggatctgcgc gtcggtcaga
     1741 gcacgttcgc catcggcaat ccgttcggtc tcgactggac gctgacgact ggcatcgtat
     1801 cggcgctgga tcgatcgttg ccgaccgagg acgggcgcgg cctgatcgag cacctgattc
     1861 agaccgatgc agcgatcaat ccgggcaact ccggcggacc gctgctcgac agcgcggggc
     1921 ggctgatcgg cgtcaacacc gttatcttca gtccgtcagg agcatcggcc ggcgtcggct
     1981 tcgcgattcc ggcggacacg gtgaatcgtg tcgtgccgca gttgatcgcg tcaggccgct
     2041 accttcgtcc ggcgctcgga atcgagatcg atgacggact gaatcagctc atctcgcagc
     2101 gtctgggcgt tgctggcgtc gcgatcctgc gaacgtcacc gggctcggct gccgcgacgg
     2161 cgggcctgcg ggggctgcgc accgagagag acggcgcgat tcttcctggc gacatcatcg
     2221 ttgccgtgaa cggccaggcc atcgacagcg tcgcgcggct gcaaagccgg ctggacgact
     2281 acaacgtcgg tgacaccgtg cggctcaccg tgctgcgcga caagcagcgc gtggacgtgc
     2341 cggtgcggct gcaagccagc acccagtaga gtatgcgctg tgctcgtctc gtgcgcgagt
     2401 gctgccggcg cgggctgctc gtcctcggcg cgggacagaa cgccatccgg ctgttgccgc
     2461 cgctcgtgct gaccgcctcg caggcggata ccgcgatcgg gatcctcgat caggcgctgg
     2521 cagcgattcg gctctaggct ctcggctttg gactctcggc tctggcctcg gggaggacgg
     2581 tatgcgcagc atcgtagtgg ccctcgtgat cgtgacagga gtcacggcta tggcccatgc
     2641 cgagactcga accgtgactt tcaatcagga tccggtgaat cagccaccga aaggattcga
     2701 gttcgggctc accgccgctg tgggcaagcc gggcgcatgg gtcgtgcagg cggagggtag
     2761 caacaggttc ctggcacaga ctgcccccga ttcgacgcgt tcgcggttcc ctctcgccgt
     2821 tctgtccgac gtgactgccg gagacg
//
LOCUS       DBNGSH010000499         5242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3273448, whole genome shotgun sequence.
ACCESSION   DBNGSH010000499 DBNGSH010000000
VERSION     DBNGSH010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3273448"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..899
                     /locus_tag="AC16CY_11470"
     CDS             258..899
                     /locus_tag="AC16CY_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3354456.1"
                     /translation="MLKRGLLTVSLVGVLLGVNVLPAEAGPITGGFSITGNFLPVNGV
                     TGAVTSLSQATGIDFLALFGSTPTPGTAGQFLVNSAAGDFGGLLGGLGTIRDISFAGP
                     GSGNFPNPNVVGPLLAFQNVLGTTFTLTSVGPASVQSFGSLSFLTFTGQGYFSMAGFS
                     NTNGTFGFTGNGAGGTFSFSASNVTVPEPASAALLGSGLVMLFGLRNRRRKTA"
     gene            1017..1862
                     /locus_tag="AC16CY_11475"
     CDS             1017..1862
                     /locus_tag="AC16CY_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354457.1"
                     /translation="MRSLLLFGCLSGAACLSGACAAATSGPAVLSIPDVRAPPVRSGE
                     LIMGYDLAVVSIAAIMERDLGLPKVRASLYFYSNRTAFRTALEGQGYTPEFARDAAAT
                     LSGIGGFRRVLLNDQSLRWLEWPYRIGLLAHELTHTVQYEFSGGRRSTSEQWLREGFA
                     EWVEVSVLEALDLTTWEQARSITVQRVRDAARRGSLPTFPEMVTYPDWVRVIMRGSEE
                     GVYAQALMAVDLLISRHGRAAAIDYFRLFAQSEDRLANFQSAFGEELSAFDRAFREHL
                     ARTLR"
     gene            1884..2417
                     /locus_tag="AC16CY_11480"
     CDS             1884..2417
                     /locus_tag="AC16CY_11480"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HIR3354458.1"
                     /translation="MVGAIREWLGLGSVETPEQAPLRDLVDALDQLEPDRARYLARFA
                     YLLGRVAHADRHVSPGETQAMEALVMEEGGITPDQAMLVVSLAKTSNLLFGGTADFEV
                     AREFADAASHDDKRALARCVFAVAASDEAISMSEETEIHRILNQLRIEPAELTALRVA
                     HRRFLPGVSKSRGDVRS"
     gene            2443..4071
                     /locus_tag="AC16CY_11485"
     CDS             2443..4071
                     /locus_tag="AC16CY_11485"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR3354459.1"
                     /translation="MASRTRPLYLGLDSSTQSLSAVVVEVQGDERRVVLETSLGFDQS
                     LPHYRTTHGVLPRSDPAVALSPPLMWVEALDVMFARLIESGLDMNRLVAVSGSAQQHG
                     SVYLNAESAGRLNALDPLRPLAEQIRPALARQVSPIWMDSSTGEQCAQIAAAVGGQGA
                     LARHTGSRAFERFTGPQIRKFFQDDPAGYSATYRVHLVSSFLSSLLLGRHSPVDPGDA
                     SGMNLMDIRSKSWWTSALEATAPGLLSKLPLIAPSWTVAGTIAPYWRRRYGLPEVKVV
                     VWSGDNPCSLVGVGLVREGRVAVSLGTSDTVFGLMKAPAADPGGHVFGAPTGDYMGLT
                     CFSNGSLARERIRDTFGLTWQEFSRALDATPPGNRARILLPWFEPEITPSVTRPGVHR
                     YGLAPDDAAANVRAVVEAQQMAMALHSGWMSVPIDTIHATGGAAVNMQILQVMADVFG
                     AEVRRLEVGNSAALGAALRAVHADGLSEGRETSWDDVVRGLAEPAADTRLKPDPRRHA
                     MYRELMPAYAACEAHALGRGIDPSPMLQRLRLGR"
     gene            complement(4093..4563)
                     /locus_tag="AC16CY_11490"
     CDS             complement(4093..4563)
                     /locus_tag="AC16CY_11490"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3354460.1"
                     /translation="MSLIDPGKKAPAFTLKDQHGKPHRLADYAGRPVVLYFYPKDDTP
                     GCTQETCDFQSRLQTFTPKKAVVLGVSILDEKSKAKFAAKHGITFPLLADADHETAEK
                     YGVWQEKSRYGRTYMGIVRTTYLIGPDGKIARRWDKVKVDGHADEVATAAAALQ"
     gene            4633..>5242
                     /locus_tag="AC16CY_11495"
     CDS             4633..>5242
                     /locus_tag="AC16CY_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3354461.1"
                     /translation="MTASRRAIIAADGVFTYDDLDESSRRVSGALLADNEDLKETRVA
                     FLVTPGFAYAAVQRGIWRAGGVAVPLAVSHPPAELDYVIRDSDASLVVGDATTAAALM
                     PLARAARARFVRAEDAMRAAPAEGLPHLGSNRRAMIIYTSGTTGRPKGVVTTHQAIGA
                     QIASLVEAWRWTPADHLLLVLPLHHVHGIINGLGSALAVRATC"
BASE COUNT          782 a         1753 c         1739 g          968 t
ORIGIN      
        1 tcgttgtcga tctgttgctc ggcgttgtgg actgaggcga gggccgactt aggcccggca
       61 gggcccctaa tgtcagcccg gggcgcgact aagtgaggat tttcccggtg acagatttct
      121 ctgccgatct atcaaatcac tgattctgat ggagttgtgt ctagcttgcc attacggcag
      181 agcctcggca gggagcttgc tatctcatgt tgcggaacgc gacagtatcg gtgcaccaaa
      241 gggacgtcgg aggacaaatg ttgaagcgcg ggttgctcac agtttcgtta gtgggtgtgc
      301 tgcttggagt gaacgtgctc ccggcggaag caggcccaat cactggggga ttctccataa
      361 ccggcaattt cctgccggtc aatggtgtca cgggtgccgt cacttcgctc agccaggcga
      421 ccggcatcga ctttctcgcg ctcttcggat ccacgcccac gccaggtacc gcaggtcagt
      481 tcctggtcaa ctccgccgcc ggcgactttg ggggactgct gggcgggctg ggaacgattc
      541 gcgacatctc gttcgccggg cctggaagcg ggaacttccc gaacccgaac gttgtcggtc
      601 ctctgctcgc gtttcagaac gtcctgggta cgacgttcac gctcacatcg gttggtccgg
      661 cgtcggtcca gagcttcgga agcctcagct tcctgacgtt taccggtcag ggctacttct
      721 cgatggccgg cttcagcaac accaacggta cgttcggttt tacgggtaac ggtgccggtg
      781 gcaccttctc gttctccgct tcgaatgtca cggtccccga gcccgcgtct gcggcgctgc
      841 tcggcagcgg cctcgtcatg ctcttcggcc ttcggaaccg gcgaaggaag accgcctagg
      901 ccccgggtcg ctcgcctctt caacggccgc ctccatcagg ggcggccgtt ttcttttttt
      961 gccgtagacc ggcgtagctc tgacggcggc caggtgcgag aattcggacc tgtcccatgc
     1021 gaagcttgct gctcttcggc tgcctgtccg gcgccgcctg cctgtcgggt gcctgcgccg
     1081 cggccacgtc cggcccggcg gtcctgtcga ttcccgacgt gcgcgcgccc ccggtccgtt
     1141 ccggggaact catcatgggc tacgaccttg cggttgtctc gatcgcggca atcatggaac
     1201 gtgacctcgg ccttccgaag gtacgggcct ccctgtactt ctattccaat cgaaccgcgt
     1261 tccgcaccgc cctcgagggc cagggctaca cacctgaatt cgcgcgcgat gccgccgcga
     1321 cgctgtcagg catcggcgga ttccgccgcg tgctgctgaa cgaccagtcg ctgcgctggc
     1381 tcgagtggcc gtaccgcatc gggctgctgg cgcacgagct gacccacacc gtgcaatacg
     1441 agttctcggg aggccggcga agcacctccg agcaatggct ccgcgaggga tttgccgagt
     1501 gggtcgaggt gtcggttttg gaggcgctgg atctcacgac gtgggagcag gcccggtcga
     1561 tcaccgttca gcgcgttcgc gatgccgcgc ggcgcggatc gcttccgaca tttcccgaga
     1621 tggtgaccta tcccgactgg gtgcgcgtca tcatgcgggg gagcgaggaa ggggtctacg
     1681 cgcaagcctt gatggctgtt gacctgctga tctcgcgtca cggccgcgcc gccgcgatcg
     1741 actacttccg gctcttcgcc caatcggaag accggctcgc caactttcaa tcggcattcg
     1801 gcgaggagct gtcggcgttc gaccgtgcgt tccgcgagca cctcgcgcgg acgctgcgat
     1861 gagaacggga gtacgatgac cccatggtag gcgcgatcag ggagtggctc ggcctgggca
     1921 gtgtcgaaac gcccgaacag gcaccgctgc gcgacctggt cgacgcactc gaccagctcg
     1981 agccggatcg cgcgcgctac ctcgccaggt tcgcctacct gctgggccgc gttgcgcatg
     2041 ccgaccgcca cgtcagccct ggcgagaccc aggcgatgga ggcgctggtc atggaggagg
     2101 ggggaatcac tcccgaccag gcgatgctgg tcgtgagcct cgcgaagacg agcaacctcc
     2161 tgtttggcgg tacggcggac ttcgaggtgg cgcgggagtt cgctgatgcc gcctcgcacg
     2221 acgacaagcg cgcgttggcc cgctgcgtgt tcgccgtggc cgcaagcgac gaggcgatct
     2281 cgatgtccga ggagaccgag atccaccgga tcctgaacca gctccgcatc gagcccgccg
     2341 agctcacggc cctccgcgtt gcccaccggc ggttcctgcc cggggtttcg aaatcgcggg
     2401 gagacgtgcg atcatgacgg cccccctcga ttgaccgttc gggtggcaag ccgcacacga
     2461 cctctctatc tcgggctcga ctcgagcacg caaagcctca gcgccgtcgt cgtcgaagtg
     2521 cagggcgacg agcggcgcgt cgttctcgaa acctcgctcg gcttcgacca gtcgctgccg
     2581 cactaccgca cgacgcacgg cgtcctgcct cgcagtgatc ccgctgttgc gctctcgcca
     2641 ccgctcatgt gggtggaggc gctcgacgtg atgttcgcgc gccttatcga gagcggcctc
     2701 gacatgaacc gcctggtggc cgtttcggga tcggcgcagc agcacggcag cgtgtacctc
     2761 aacgccgaat cggcgggccg cctgaacgcc ctcgatccgc tgcgtccgct cgccgaacag
     2821 atccggccgg cgctggcacg acaggtgtcg cccatctgga tggattcgag caccggggag
     2881 cagtgcgcgc agatcgccgc ggccgtcggc ggacagggcg cgctcgcgcg ccacaccggg
     2941 tcccgcgcgt tcgaacgctt cacggggccg cagatcagga agttcttcca ggacgatccg
     3001 gcgggctact cggcgacgta tcgcgtgcac ctggtcagtt cgttcctgtc gtcgctgctc
     3061 ctgggccggc acagcccggt cgatcccgga gacgcgtccg gcatgaacct gatggacatc
     3121 cgctcgaagt cctggtggac gtccgccctc gaggccacgg ccccagggct gctgtcgaag
     3181 ctgcccctca tcgctccctc gtggacggtg gcagggacca ttgcaccgta ctggcggcgg
     3241 cgttacggcc tgccagaggt gaaggtcgtc gtgtggtcgg gggacaaccc gtgcagcctt
     3301 gtcggcgtcg gcctcgtgcg cgaaggccgc gtcgccgtgt cactgggaac gagcgatacg
     3361 gtcttcggtc tgatgaaagc gccggctgcc gatcctggcg gccacgtgtt cggcgcgccg
     3421 accggcgact acatgggcct gacgtgcttc agcaacggat cgctcgcgcg cgaacggatc
     3481 cgcgacacat tcggactcac gtggcaggaa ttctcgcgcg cgctcgacgc gacgccgccg
     3541 ggcaaccgcg cgcggatcct cttgccgtgg ttcgagcccg aaatcacgcc ttccgtgacg
     3601 cggcccggcg tgcatcgata cggcctcgcg cccgacgatg cggcagccaa cgtccgagcg
     3661 gtcgtggaag cgcagcaaat ggccatggcg cttcactcgg gctggatgtc ggtgcctatc
     3721 gacacgatcc atgcgacagg gggcgccgcc gtgaacatgc agatcctgca ggtgatggcc
     3781 gacgtgttcg gggccgaggt ccgtcggctc gaggtgggca actcggcggc gctgggcgcg
     3841 gcgctccgag ccgttcacgc cgacggcctt tcggagggac gtgaaacctc ctgggacgac
     3901 gtcgtgcgcg gccttgcgga gccggccgcc gatactcgat tgaagcccga cccgcgccgc
     3961 cacgcgatgt atcgcgagct catgccggcc tacgcggcct gcgaggcgca cgcccttggc
     4021 cgcggcatcg atccgtcgcc gatgctgcag cgcctccggc tcggtcgctg atctgtcgcc
     4081 tgcctgttca ggctactgca acgcggccgc ggccgtggcg acttcgtcgg cgtggccgtc
     4141 caccttcacc ttgtcccagc gcctggcgat cttgccgtcg ggtccgatga gatacgtcgt
     4201 gcgaacgatg cccatgtagg tacgtccgta ccgcgacttc tcctgccaca cgccgtattt
     4261 ctcggccgtc tcgtgatcgg cgtctgccag cagcggaaat gtgatgccgt gcttggcggc
     4321 gaacttcgcc ttgctcttct cgtcgaggat gctcacgccg aggacgacgg ccttcttcgg
     4381 cgtgaacgtc tggagccgcg actggaaatc gcacgtctcc tgcgtgcagc cgggcgtgtc
     4441 gtccttcggg tagaagtaga gcacgacggg acgtcccgcg tagtcggcca gccgatgtgg
     4501 ctttccatgc tggtccttca acgtgaacgc cggggccttc ttgccgggat cgatcaggga
     4561 catgtcgaga tctccaagag caactgtagg ggtctacccc tgcagatagt tgcacatagt
     4621 attgtgttct cgatgacagc ttcccgccgg gcgatcattg ctgccgacgg cgtcttcacc
     4681 tacgacgatc tggacgagtc ctcgcgtcgc gtgtcgggtg cgctgctcgc cgacaacgag
     4741 gacctcaagg agacgcgtgt cgcgtttctc gtgactcctg gcttcgcata tgccgccgtg
     4801 cagcgaggca tctggcgagc aggcggcgtg gcggtcccgc tggccgtttc gcatccgccg
     4861 gccgagctcg actacgtgat ccgcgactcg gacgcgtcgc tggtggtcgg cgacgcgacc
     4921 acggcggcgg cactgatgcc gctggcgcgc gcggctcgcg ccaggttcgt gcgcgccgag
     4981 gacgcgatgc gcgccgcacc ggcagagggt cttccgcatc tcggctcgaa ccgccgagcg
     5041 atgatcatct acacgagcgg cacaacaggc cgcccgaagg gggtggtgac gacgcaccag
     5101 gcgatcggcg cgcaaatcgc gtcgctcgtc gaggcctggc gctggacgcc cgccgaccac
     5161 ctgctcctgg tgctcccgct tcaccacgtg cacggcatca tcaacgggct cgggtcggcc
     5221 ctcgccgtgc gcgccacctg cg
//
LOCUS       DBNGSH010000500         7467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1745543, whole genome shotgun sequence.
ACCESSION   DBNGSH010000500 DBNGSH010000000
VERSION     DBNGSH010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1745543"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2004
                     /locus_tag="AC16CY_11500"
     CDS             <1..2004
                     /locus_tag="AC16CY_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3354462.1"
                     /translation="FAQGYSTRRTEVRAAAGAPSIDLQVDFDLHFEEVVSVSGDARSQ
                     FESFQATSVLAGQELSKQLEMSLGATLENQPGVAVRSFGPAPSRPVVRGLDGDRVLIL
                     QDGQRLGDLSSQSGDHGVAINPASAQKIEVVRGPATLLYGSNAIGGLVNIITDEIPTR
                     PQMGARGNFTFDLGSAANEGGAAGDVNIGNGSVALHMGGGGHRSSDVGTPEGELANSQ
                     SRNGFGSVGLSWTGSKGYVGGSYGYDDTKHGVPIVEEGQVQLTPRRHAFSLRGGAQGL
                     EGAFDSYRATLAVRRYKHEELEADEVGTAFANDTEEVEVMASHRTLGRLKGSVGGWFL
                     NRAFDAVGEEALSPAVDQRGFAAFLYEEVTWPHVTFQFGARVDDATYEPNGEPARDFT
                     TGSGSLGLLLRPGAADDRITFAVSLAYASRIPALEELFYFGPHPGNFAFEVGNPGLDP
                     EKALGLDVALRWRTARASGEVAYFRNDINDFVFRAPLTEEEFVARIDEFAARFPGRGI
                     EDEIPSTEGFPIIENVAADSLLQGLEAHSDLYVTTNVIVELGLDYVRGTLKATDQPLP
                     RMPPLRFRTGLRYQRNAFQAGGDVTIAATQDRVFENETPTDGYQLLRLFGSYSFGRGP
                     VAHTITARLDNATNELYRNHLSLIKDLAPEMGRNFKLLYNVRF"
     gene            complement(2045..2764)
                     /locus_tag="AC16CY_11505"
     CDS             complement(2045..2764)
                     /locus_tag="AC16CY_11505"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3354463.1"
                     /translation="MSAEIQTSRSDLRRRPQAEEGALTILYEDDRIIVLDKPPGVVVH
                     PTYKNTSGTLLNAVLWHVRGHAGVQPGILTRLDKNTSGLVIVALTPEVHALMQKHAAS
                     GRVSKHYLALVRGVPEPRTGRIDLPLARDAGDRRRVVVTPAGASCETRYDVMGTDGGV
                     SLVRCEPVTGRTHQIRVHLSASGWPIVGDAVYGAPDDSIARQALHAWRVSLPHPVTGD
                     HLQFESPLPPDMLRLCPRRYL"
     gene            2797..3447
                     /gene="msrA"
                     /locus_tag="AC16CY_11510"
     CDS             2797..3447
                     /gene="msrA"
                     /locus_tag="AC16CY_11510"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3354464.1"
                     /translation="MAIFSSPMKLRMPLPDQALPGRDTPMAVPVTHAVNGARLTPPFP
                     DGLERAVFAMGCFWGAERKFWQAPGVYSTAVGYAGGYTPNATYKEVCSGMTGHTEVVL
                     VVFDPKTNSYDEMLKVFWENHDPTQGMRQGNDVGTQYRSAIYCFSPAQRRAAEASRAA
                     FQDRLTAAGFDRITTEIADAPPFYYAEDYHQQYLHKNPDGYCGLGGTGVSCPVGVG"
     gene            3497..3928
                     /locus_tag="AC16CY_11515"
     CDS             3497..3928
                     /locus_tag="AC16CY_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HIR3354465.1"
                     /translation="MVTMTQIELVQTSFAVIAPIADDAAALFYRRLFEIDPSLRQMFP
                     GDLSEQRKKLMQMLTAAVKGLPRLDRLVPVVEDLGRRHTGYGVRDEHYETVGAALLWT
                     LEKGLGDAFSPDVREAWTAVYGVLASTMKRAAASQAAAAAA"
     gene            complement(3968..5290)
                     /locus_tag="AC16CY_11520"
     CDS             complement(3968..5290)
                     /locus_tag="AC16CY_11520"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3354466.1"
                     /translation="MNDSIGPAEAIFLSLADLGADEREAELRERCGDDSRLRAEVDAL
                     LAAIDSPDDGFLDPARVPSLDMGAVDGPLQPGTVLGGFLVLHAIGSGGMGVVYAAEQD
                     RPRRTVAIKVLRRNFRHPEILRRFEREAEVLGRLQHPNIARVFAFHHGDRRIPAHLVM
                     ELVDGPPITEYVQAQALGHEARIDLMIAVCEAVQHAHERGVVHRDLKPANVLVSREGR
                     PTVLDFGIARATGLDIHSTVHTRHGQLLGTLAYMSPEQLRGAKGEVDGRSDVYALGAM
                     LYRLLAGRLPFDVGGLSLLEAAQQILHAEVPSLGTVDATLAGSIEQVVGRAMSQDRER
                     RYQSAADLAADLRACLDGRGPTQSDAADRPSARVLVAESADHRFVALGLRTGAVVVLD
                     AASGARLTAVEASGRLERLAFNAESRLVIGRSNGRVQVVLLEDLRPEP"
     gene            complement(5287..5853)
                     /locus_tag="AC16CY_11525"
     CDS             complement(5287..5853)
                     /locus_tag="AC16CY_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3354467.1"
                     /translation="MTSVPRDPDQPGTSGEMLPEVYDELRKLARARLARERQPHQTLQ
                     PTALVHEAYLRVSDQPREWERRGHFFAAAALAMRRILVERARHYRRIKHGAGGEHVEI
                     DEALAAADPDLTNVLAIDEALSRLEVNDPRKAKIVTLRYFAGLSIEETAAALDLSPAT
                     VKNEWTFARAWLFRTLGSSGDTRADDPE"
     gene            5868..6311
                     /locus_tag="AC16CY_11530"
     CDS             5868..6311
                     /locus_tag="AC16CY_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012272.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HIR3354468.1"
                     /translation="MTPESIARLEASFAEVSARPRALATRFYQELFSAAPALRPLFPA
                     DMTSLQGHFEAALALVIKNLEDMSALQESLRDLGAQHVHWGAKPGDYFVVRGALVRAI
                     RASSASWTPELEADWHRAITAIAVPMLQGAAVHTAVVAEQMAEKP"
     gene            6341..6766
                     /locus_tag="AC16CY_11535"
     CDS             6341..6766
                     /locus_tag="AC16CY_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354469.1"
                     /translation="MSRVVYSDLQSTADQESLRLAHQVRIVNTAEEGTGVNALPSGVY
                     GFTYSPGLPSAPLFAVRRYRSYETHKLASGEVYVIGFASADAARTLSTTAEEFTLQIH
                     PQPENEYDVLVTIPYSSMRRHRQYAAPNQDGFTVTVMPV"
     gene            6923..>7467
                     /locus_tag="AC16CY_11540"
     CDS             6923..>7467
                     /locus_tag="AC16CY_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354470.1"
                     /translation="MTRMALLVLGALAVSSSAMAQTPAETIERALAAAPRQMREGAAV
                     IKWKPDFTYDTLKPGTNRLVCYDRSGEPGEQPFAVQCTSVGNLERVAQNRRIEVEQDR
                     AKRQAAFDAAEANGTRVKPEYGSVFYSLDGKDQATARLHTTIAVPGATTQSTGLPDNG
                     KAGGAWIMNAGTTTAHIMTPG"
BASE COUNT         1266 a         2463 c         2551 g         1187 t
ORIGIN      
        1 ttcgcgcagg ggtactcgac caggcgcacc gaggtgcgtg ccgccgcagg cgcgccgtcg
       61 atcgatctgc aggtggactt cgacctgcac ttcgaggagg tcgtgtcggt gagcggtgac
      121 gcgcgcagtc agttcgagtc gttccaggcg acgtcggtgc tggcggggca ggagctgtcg
      181 aagcagctcg agatgtcgct cggcgcgacg ctcgagaatc agcccggtgt cgccgttcgc
      241 agcttcggtc cagcgccttc gcggcccgtc gtgcgcggcc tcgacggcga tcgcgtgcta
      301 atcctgcagg acgggcagcg gctcggcgac ctttcgtcgc agtccggcga ccacggcgtg
      361 gcgatcaacc cggcgtcggc ccagaagatc gaggtcgtcc gaggtcccgc cacgctcctg
      421 tacggctcca acgcgatcgg aggcctcgtc aacatcatca ctgacgagat tccgacgcgc
      481 ccgcagatgg gagctcgcgg caacttcacc ttcgatctcg gatcggcggc gaacgaggga
      541 ggcgctgcgg gagacgtcaa catcggcaac gggtcggtcg cgctgcacat gggcggcggc
      601 ggccatcgat ccagcgacgt ggggacaccc gaaggtgagc tcgccaactc gcaatcccga
      661 aacggcttcg gcagcgtcgg tctctcgtgg acgggctcga aagggtacgt cggcggcagc
      721 tacggctacg acgacacgaa gcatggcgtc ccgatcgtcg aggaagggca ggtgcagttg
      781 acgccgaggc ggcatgcttt ctcgctgcgc gggggcgcgc aggggttgga aggcgcattc
      841 gactcctacc gcgccacgtt ggccgtgcgg cgatacaagc acgaggaact cgaggccgac
      901 gaggttggga ctgcgttcgc gaacgacact gaagaagtcg aggtgatggc ctcgcatcgc
      961 acgttggggc gcctcaaagg gagcgtgggc ggctggttcc tgaaccgggc gttcgatgcc
     1021 gtcggcgagg aggcgctgtc tccggcggtc gatcaacgcg ggttcgccgc gttcctgtac
     1081 gaggaagtga cctggcccca cgtgaccttc cagttcggcg cccgcgtcga tgacgcgacc
     1141 tacgagccga acggcgagcc cgcgcgcgac ttcacgacgg gatcggggtc gctcggcctg
     1201 ctgttgcggc ccggcgcggc cgacgatcgg atcacgttcg cggtcagcct ggcatacgcc
     1261 tctcgaatcc ccgcgctcga ggagctcttc tacttcgggc cgcatcccgg caacttcgcc
     1321 ttcgaggttg gcaacccggg gctcgatccc gagaaagcgc tcggcctcga cgtggcgctg
     1381 cgctggcgca cggcaagggc ttcgggcgag gtcgcctact tccgcaacga catcaacgac
     1441 ttcgtgttca gggcccctct gacggaagag gagttcgttg cgcgcatcga cgagttcgcg
     1501 gcccgctttc ccggccgcgg tatcgaagac gaaataccgt caaccgaagg gttccctatc
     1561 atcgagaatg tcgccgccga cagcctcctg cagggactcg aggcgcactc ggacctctac
     1621 gtgaccacga acgtgatcgt cgagctcggt ctcgactacg tccgtggcac gctcaaggcc
     1681 acagaccagc cgctgccgcg gatgcccccg ctgcgcttcc gaaccggtct ccgttatcag
     1741 cgaaacgcct ttcaggcagg aggtgacgtc actatcgccg cgacgcagga tcgagtgttc
     1801 gagaacgaga cgccaactga cggataccag ctgctgcggc tcttcggctc gtactccttc
     1861 ggccgcggtc cggtggcgca cacgatcacc gcgcggctcg acaacgcgac gaacgagctg
     1921 taccggaacc atctgtcgct catcaaggat ctggctccgg agatggggcg gaacttcaag
     1981 ttgctgtaca acgtgaggtt ctgaggtgac gacctgtgaa ggggtctgac cctttcacgc
     2041 gctttcacag atatcgccgc gggcagaggc gaagcatgtc cggcgggagc ggtgactcga
     2101 actgcaggtg atcgcctgtc accggatgcg gcaagctgac ccgccacgca tggagcgcct
     2161 gtcgggcgat cgagtcgtct ggcgcgccgt aaaccgcgtc gccgacgatt ggccagccgc
     2221 tcgccgacag gtgaacacgg atttgatggg tgcggccggt gacaggttcg caccgcacga
     2281 gcgacacgcc gccatcggtc cccatgacgt cgtagcgcgt ctcgcacgag gcccccgcgg
     2341 gcgtgacgac cacgcgcctg cgatcgccgg cgtcccgcgc gagcggcagg tcgatgcgcc
     2401 cggtgcgcgg ctcgggcaca ccgcgcacga gcgccaggta gtgcttgctg actcgccccg
     2461 acgcggcgtg cttctgcatc agggcgtgca cctcgggcgt gagcgccacg ataacgaggc
     2521 cggacgtgtt cttgtcgagc cgggtcagga tgcccggttg tacgccggcg tggccgcgca
     2581 cgtgccagag cacggcgttg agcagggtgc ccgacgtgtt cttgtaggtc ggatggacga
     2641 cgacgccagg cggcttgtcg aggacgatga tccggtcgtc ttcgtagagg atggtgagcg
     2701 cgccttcctc agcctgtggc cgccgcctga ggtcggaccg cgacgtttga atctcggcgc
     2761 tcatttcagg catcttaacg gagacaagag gaccacatgg cgatattcag cagcccgatg
     2821 aagcttcgaa tgccgttgcc cgatcaggca ttgcccgggc gcgacacgcc gatggccgtc
     2881 cccgtgacgc atgcggtgaa cggcgcacgg ctcacgccgc ccttccccga tggactcgag
     2941 cgggcggtgt ttgcgatggg ctgcttctgg ggtgccgagc ggaagttctg gcaggcgccc
     3001 ggcgtctatt cgacggctgt cggctatgca ggaggctaca ctccgaacgc gacctacaaa
     3061 gaagtctgct cgggaatgac cgggcacacc gaggtcgttc tggtcgtttt cgatccaaag
     3121 accaacagct acgacgagat gctcaaggtg ttctgggaga atcacgaccc gacgcagggc
     3181 atgcgtcagg gcaacgacgt aggcacgcaa taccgatcgg cgatctattg tttctcgccg
     3241 gcccagcggc gcgcggccga agcatcgcgg gcggcgtttc aggaccggct gactgcggcc
     3301 ggctttgacc gcatcacgac ggaaatcgcc gacgccccgc ccttctacta cgcggaggac
     3361 tatcaccagc agtacctgca caagaacccg gacggctact gtgggctggg cggtaccgga
     3421 gtaagctgtc cggtcggggt cggttagcca ctttccgccg gaacgtcgca ttgggtatca
     3481 gagaaggaga tatccaatgg tgacgatgac ccaaatcgag ctggttcaaa cgagcttcgc
     3541 cgtaatcgcg ccaatcgccg acgacgcggc ggcgctcttc tatcggcggc tcttcgagat
     3601 cgatccgagc ctccggcaga tgttccccgg cgacctgtcc gagcagcgca agaaactgat
     3661 gcagatgctg acggctgcgg tcaaggggct cccgcgcctc gatcggctcg tgcccgtcgt
     3721 cgaggatctc ggccgccggc acaccggcta cggcgtgcgc gacgagcact acgagacggt
     3781 gggggcagcc cttctctgga cgctcgaaaa ggggctcggc gacgcgttct cgcccgacgt
     3841 gagggaagcg tggaccgcgg tgtacggcgt gctggcctcc accatgaagc gggcagccgc
     3901 cagccaggcg gccgctgccg cggcctagga ggacagggcc cagggctcag ggctcagggc
     3961 ccagtgctca gggctcgggc ctcaggtcct cgagcaggac gacttggaca cggccgttgg
     4021 accggccgat gaccagtcgc gactcggcgt tgaatgccag ccgttcgagc cttccgctgg
     4081 cttcaacggc tgtgagccgc gcgcccgatg ccgcatcgag gacgacgacc gcccccgtcc
     4141 tcaacccgag ggcaacgaac cggtggtcgg cgctttcggc gacgaggacc cgtgccgacg
     4201 gccggtcagc ggcatcggac tgcgtcgggc cgcggccatc gagacaggcg cggagatcgg
     4261 cggccagatc cgcggcggac tgatagcggc gctcacggtc ctgtgacatt gcccgtccga
     4321 caacctgctc gatcgatccg gcgagcgtgg catcgacggt gccgagcgac ggtacctcgg
     4381 cgtgaagaat ctgctgagcc gcttcgagaa gcgagagccc gccgacgtcg aacggcagac
     4441 ggccggccaa cagccggtag agcatcgcgc ccagcgcgta cacgtcgctc cggccgtcga
     4501 cctcgccctt ggcgccgcgc agttgctccg gactcatgta ggcgagcgtg ccgaggagct
     4561 gtccgtgccg ggtgtgaacg gtggaatgaa tgtcgagccc cgtggcgcgc gcgatgccga
     4621 agtcgagcac ggtcggccgc ccctctctcg acacgagcac gtttgcgggc ttcaggtctc
     4681 gatggacgac gccgcgttcg tgcgcgtgct gcaccgcctc gcacaccgcg atcatcagat
     4741 cgatgcgcgc ctcgtgaccg agtgcctgcg cctggacgta ctccgtgatt ggcggcccgt
     4801 cgaccaactc catgacgagg tgcgccggga tccgccggtc tccgtgatgg aacgcaaaca
     4861 cccgtgcgat gttcgggtgc tggaggcggc cgagcacctc ggcttcgcgc tcgaatcgcc
     4921 tcaggatctc gggatgacgg aaattccgcc gcagcacttt gatcgcgacg gtcctgcgag
     4981 ggcggtcctg ctcggcggcg tagacgacac ccattccccc ggacccgatt gcgtgaagga
     5041 cgagaaagcc gccgaggacg gtgcccggct ggagcgggcc atcgacggcc cccatgtcga
     5101 gcgacggcac ccgcgccgga tcgagaaatc catcgtcagg ggaatcgatt gccgcgagca
     5161 gggcgtcgac ctccgcgcgc agccgggagt cgtcaccgca tctttcccgg agctccgctt
     5221 cgcgctcgtc ggcgccgaga tcggcgagcg agaggaagat ggcctcggcg ggaccgatgg
     5281 agtcgttcat tccgggtcgt ctgcacgcgt gtcacctgac gagccgagcg tgcgaaagag
     5341 ccaggcgcgc gcaaacgtcc actcgttctt gacggtggcg ggcgacaggt cgagcgccgc
     5401 ggcggtttcc tcgatgctga ggccggcgaa gtaccgcagc gtgacgatct tcgcctttcg
     5461 cgggtcgttg acctcgaggc gggacaaggc ttcgtcgatg gcgaggacgt tggtcagatc
     5521 cgggtccgcg gccgcgagcg cttcgtcgat ctccacgtgc tcgccgcctg caccgtgctt
     5581 gatgcggcgg tagtggcgcg cgcgttccac gaggatgcgg cgcatggcga gggcggcggc
     5641 cgcgaagaaa tgaccgcgcc gctcccactc gcggggctga tccgacacgc ggaggtacgc
     5701 ttcgtggacg agggccgtcg gctgcagcgt ctggtggggc tggcgctcgc gggcgaggcg
     5761 cgccctcgcg agcttccgca gttcgtcata cacttccggc agcatctcgc cggaggtgcc
     5821 cggctggtcg ggatcgcggg ggacggatgt cacaaagcgg cgcaattatg acacccgaaa
     5881 gcatcgctcg gctcgaggcc agcttcgccg aggtatctgc gcggccgcgc gccttggcca
     5941 cgcgcttcta tcaggagctc ttcagcgcgg cgccggcgct ccggccgctg tttcccgccg
     6001 acatgacgtc gctccaggga cacttcgaag cagcgctggc gctggtcatc aagaacctcg
     6061 aagacatgtc cgcgctgcag gaatcgctca gagacctcgg cgcgcagcac gtgcactggg
     6121 gcgccaagcc tggagactac ttcgtcgtgc gcggtgcgct cgtccgcgcc atccgcgcct
     6181 cgtccgcctc gtggaccccc gagctcgaag ccgactggca tcgcgcgatc acggccatcg
     6241 ccgtcccgat gctgcagggc gccgccgtgc atactgcggt cgttgccgag cagatggcag
     6301 aaaagccctg agcgcggggt gaacggtata atcgttcccc atgtcccgcg tcgtctattc
     6361 cgacctgcag tcaaccgccg accaggagtc cctgcgactg gcccatcagg tgcggatcgt
     6421 gaacacggcc gaggaaggga ccggcgtcaa cgcgctgcct tccggcgtct acgggttcac
     6481 ctactctccg ggcctcccga gcgcgccgct gttcgcggtg cgtcgctatc gcagctacga
     6541 gacgcataag ctggcgagcg gcgaggtcta cgtgattggc ttcgcgagcg cggacgccgc
     6601 acgcacgctc tcgacgacgg ccgaggaatt cacgctgcag atccatcccc agccggagaa
     6661 cgagtacgac gtgctcgtga cgatcccgta ctcgagcatg cgccggcatc gccagtacgc
     6721 cgcgccgaac caggacgggt tcaccgttac cgtgatgccc gtctagtccc atccctcgtc
     6781 cctggtcctc tacggaccca ggaccaacca ccaaggacgg actcgggacc aagaacggac
     6841 acaggaccaa gcccgaagga ccagggacca ggcacgcgtg tatactccgt cctgtcgccg
     6901 acattcaccg gaggtcacca acatgactcg catggctctt ctcgttctcg gcgcgctggc
     6961 ggtctcttcc agcgcaatgg cgcagacgcc ggcagaaaca atcgagcgcg ccctggcggc
     7021 cgcgccgcgc cagatgcgcg aaggggctgc cgtcatcaag tggaagcccg acttcaccta
     7081 cgacacgctg aagccgggca cgaaccgtct ggtctgctac gaccgttccg gcgaacccgg
     7141 cgagcagccg ttcgcggtcc agtgcaccag cgtcggcaac ctggagcgcg tggctcagaa
     7201 ccggcggatc gaggtcgagc aggaccgggc gaagagacag gccgcgtttg acgccgccga
     7261 ggcgaacggg acgcgggtga agcccgagta cgggtcggtc ttctactcgc tcgacggcaa
     7321 ggatcaggcg acggcccgcc tgcacacgac aatcgcggtg ccgggtgcga cgacgcagtc
     7381 aaccggactg cccgacaacg ggaaggctgg cggcgcctgg atcatgaatg cggggacgac
     7441 gaccgcgcac atcatgacgc cggggca
//
LOCUS       DBNGSH010000501         4684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1235185, whole genome shotgun sequence.
ACCESSION   DBNGSH010000501 DBNGSH010000000
VERSION     DBNGSH010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1235185"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="AC16CY_11545"
     CDS             complement(<1..1151)
                     /locus_tag="AC16CY_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3354471.1"
                     /translation="MPFGYHGSYLRIDVSSGRAERVPLSETVLRQYIGGSGLGARLLL
                     ENGVGSHFPTDVPLENDSRPHFPDPLSPEAPLVFAFSPLVGSPLTTSAKFAVVSRSPL
                     TERFNDSLASSGFALAGKRCGCDAIVIVGRAREWSVLVVDDGEVRLEPAGEILGDPCH
                     VAEEKLRARLGADHRIASIGPAGERAVRYATISHDGRHAGRGGSGAVLGSKAVKAIAV
                     RGTKYCEWAQPRELTELSRDLSKRSFGPATAKYRELGTATNLLTFNRFGTLPTRNFQS
                     GTFEEAPNLSPDQLSIAHARTRSSCVACTIGCEHIYGIGGRGVRLEYESMFALGSLCG
                     VGDGDAVLRASQRCDELGIDTISTGGTIAFAMECVERGLLDEPWLTFGSG"
     gene            complement(1162..1416)
                     /locus_tag="AC16CY_11550"
     CDS             complement(1162..1416)
                     /locus_tag="AC16CY_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3354472.1"
                     /translation="MHVEFLGIPRQRAGVSELEVEARTLGQLLDALALRCPALGELIT
                     PQGLHPSLSANLNGDAFVRDPDTPLAKGDTLLILSADVGG"
     gene            1484..3205
                     /locus_tag="AC16CY_11555"
     CDS             1484..3205
                     /locus_tag="AC16CY_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HIR3354473.1"
                     /translation="MSDRTPTSKAVASPKGKRSRVRSTPKGRAVNPQALEEVRELLGN
                     APRRRDLLIEHLHKVQDRFGCLSAAHLVALASEMKMAMAEVYEVATFYHHFDVVKEGD
                     TAPPAITVRVCDSIACDMAGSHDLLKTLPGRLGAGVRVLHAPCVGRCESAPVAVVGQN
                     PVPHATAEKVVSLVKAGAVRHSPSEIAITPGHIDYAAYRAAGGYALAASCVGGARTAG
                     DVMALMEDSGLRGLGGAGFPAGRKWKIVSAEPSPRLMAINIDEGEPGTFKDRYYLERD
                     PHRFLEGAIIAAWAVGIDAVYVYLRDEYHGCRAILERELAALRADPPCPIPEFHLRRG
                     AGAYICGEESAMIESIEGKRGEPRLRPPYVAQVGLFGRPTLEHNMETLHWVREIIEKG
                     PGWFAGQGRNGRKGLRSFSVSGRVRKPGVHLAPAGITLLELIDEYCGGMLEGHTLYGY
                     LPGGASGGILPASMANVPLDFDTLNQYGCFIGSAAIVVLSDKDTAAAAARNVMKFFAD
                     ESCGQCTPCRVGTEKALHLMKARTWDGPLLQELSQAMMDASICGLGQAAPNPMLTVMK
                     YFPGEVD"
     gene            3205..>4684
                     /locus_tag="AC16CY_11560"
     CDS             3205..>4684
                     /locus_tag="AC16CY_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3354474.1"
                     /translation="MHKMLDTKMVTFTLNGQTVTAPANQTILETADDHGIDVPRLCYM
                     EGMRADGNCRTCMVEIKGERVLAASCCRYPKDGMEVTTDSARAVISQKMSIELLLSDI
                     PETSYTLDSELDLWAKKMGVGKPRFGARPQPVEDLSHPAMAVHLDACIQCKRCVRACR
                     EEQVNDVIGYAFRGGRSKIVFDLDDPMGDSTCVACGECVQACPTGALMPARDVARIVP
                     DKTVDSVCPYCGVGCQLTYNVKDNRILYVQGRDGPANSSRLCVKGRYGFDYVQHRHRL
                     TRPLIRKKGVPKHKDFTVDPEKWHEVFREVTWDEALDFAAKGLREIRDTHGKRALAGF
                     GSAKGTNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAALMEGINSGAVSNQVRDVA
                     NAEVIFVIGANPTVNHPVAATWIKNAAKAGARLIVADPRRGDLARHATYNLQFNADTD
                     VALLNAMLHVIVEEGLVDEAFIRDRTSGYAELAENVKKFSPEA"
BASE COUNT          801 a         1592 c         1581 g          710 t
ORIGIN      
        1 ccgctgccga aggtgagcca cggttcgtcg agcaggccgc gctcgacgca ctccatggcg
       61 aaagcaatcg tgccacccgt gctgatcgtg tcgatgccaa gctcgtcgca ccgctgcgac
      121 gcgcgcagca cagcgtcgcc gtcgccgacg ccgcacagcg agccgagcgc gaacatgctc
      181 tcgtactcca gccgcacgcc gcggccgccg attccgtaga tgtgctcgca gccgatcgtg
      241 cacgccacgc agctcgatcg ggtgcgcgca tgcgcgatgc tcagctgatc gggcgagagg
      301 ttcggcgcct cctcgaatgt cccgctctgg aagttgcgcg tcgggagggt gccgaagcga
      361 ttgaacgtca gcaggttcgt cgccgtgccg agctcgcggt acttcgccgt ggccgggcca
      421 aacgatcgct tcgacaggtc ccggctcagc tcggtcaact cgcgcggctg cgcccactcg
      481 caatacttcg taccgcgcac cgcgatcgcc ttgaccgcct tgctgccgag gaccgctccg
      541 ctcccgcctc ggccggcgtg ccgcccgtcg tgcgagatcg tcgcgtagcg cacggcgcgt
      601 tcaccggccg ggccaatcga ggcaatgcgg tgatcggccc ccagccgcgc ccgcagcttc
      661 tcttcggcga catggcaggg gtcgcccagg atctcgccgg ccggctcgag ccgcacctct
      721 ccgtcgtcga cgacgaggac agaccactcg cgcgcgcggc cgacgatgac gatggcgtcg
      781 cagccgcagc gcttgccggc cagggcaaag ccgctgctcg cgagcgagtc gttgaaacgc
      841 tccgtcagcg gactccggct gacaacggca aactttgcgg aggtagtgag cggactgccg
      901 acgagcggac tgaaggcgaa cacgagaggc gcctcgggcg agagcggatc tggaaaatgg
      961 ggtcgggagt cattttccag ggggacgtca gttggaaaat gactcccgac cccattttcc
     1021 aggagcagcc gcgcgcccag gccgctgccg ccgatgtact gcctcagcac cgtctcagag
     1081 agcggcacgc gctccgcgcg tccgctcgag acgtcgatgc gcagatagct cccgtggtac
     1141 ccgaaaggca tggcctggcg gctagccgcc gacgtcggcg gaaaggatca gcagcgtgtc
     1201 tcccttcgcc aggggcgtgt cgggatcgcg cacgaacgcg tctccgttga ggttcgcgga
     1261 gagcgaggga tgcaggccct gcggcgtgat gagctccccc aatgccgggc accgcagcgc
     1321 cagcgcgtcg agcaactggc cgagcgtccg cgcctcgacc tccagttcgg agacaccggc
     1381 gcgttggcgg ggaatgccga ggaattcgac gtgcatctca ggtcagatag cggaccgtaa
     1441 cgcgatgata acatggggtg ttaaccccaa tcccgggtcc tgaatgagcg atcgcactcc
     1501 cacatcgaag gccgttgcct cacccaaggg caagcgctcg cgcgtgcgct cgacgcccaa
     1561 gggacgggcc gtcaaccccc aggccctgga ggaagtgcgc gaactcctgg gcaacgcgcc
     1621 gcgccgtcgc gacctgctga tcgagcacct gcacaaggtt caggaccgct tcgggtgcct
     1681 gtcggccgcg catctcgtcg cgctcgcctc cgagatgaag atggccatgg ccgaggtgta
     1741 cgaagtggcc acgttctacc accacttcga cgtcgtgaaa gagggcgata ccgcgccgcc
     1801 cgccatcacc gtgcgcgtct gcgactcgat cgcgtgcgac atggccggat cgcacgacct
     1861 gctgaagacg ctcccgggcc ggcttggcgc gggcgttcgg gtcctccatg ccccgtgcgt
     1921 cggacgctgc gagtcggcgc ctgtcgccgt ggtcggtcag aaccccgtgc cgcacgcgac
     1981 ggccgagaag gtcgtgtcgc tggtaaaggc cggcgccgtc aggcactccc cctccgagat
     2041 cgcgatcacg cccgggcaca tcgactacgc ggcataccgc gcggccggcg ggtacgcgct
     2101 cgccgcatcg tgcgtcggcg gcgcgagaac ggcgggcgac gtcatggccc tgatggaaga
     2161 ctcggggctg cggggccttg gcggcgcagg cttccccgcc ggccgcaagt ggaagatcgt
     2221 ctcggctgaa ccgtcgccgc gcctgatggc gatcaacatc gacgaaggcg aacccggcac
     2281 cttcaaggat cgctactacc tcgaacgcga tccccaccgc tttctcgaag gcgcgatcat
     2341 cgccgcctgg gccgtcggca tcgacgctgt ttacgtctac ctccgcgacg agtatcacgg
     2401 ctgccgggcg atcctggaac gcgagcttgc cgcccttcgg gctgacccgc cgtgcccgat
     2461 tcccgagttc catctccgcc ggggcgcggg ggcctacatc tgcggcgaag agtcggcgat
     2521 gatcgagtcg atcgagggca agcgcggcga gccgcgcctg cgtcctccgt acgtcgcgca
     2581 ggtcgggctg ttcggccgcc ccaccctcga gcacaacatg gagacgctgc actgggtgcg
     2641 cgagatcatc gagaagggtc caggctggtt tgcagggcag ggacggaacg gccgcaaggg
     2701 cctgcgctcg ttctcggtga gcggacgcgt caggaagccg ggtgttcacc tggcgccggc
     2761 cggcatcacg ctgctggagc tcatcgacga atactgcggt ggcatgctcg agggccacac
     2821 cctctacggc tatctcccgg gcggcgcctc cggcgggatc ctgccggcat cgatggcgaa
     2881 cgtgccgctc gacttcgaca cgctcaacca gtacggctgc ttcatcggct cggccgcgat
     2941 cgtcgtgctg tcggacaagg acacggcggc ggcggcggcc aggaacgtga tgaagttctt
     3001 cgcggacgag tcctgcggtc agtgcacgcc gtgccgggtc ggcacggaga aggcgctgca
     3061 tctgatgaag gcgcgcacgt gggacgggcc gctcctgcag gagctgtctc aagccatgat
     3121 ggatgcctcg atctgcgggc tgggccaggc ggcgcccaac ccgatgctga cggtcatgaa
     3181 atacttcccg ggggaagttg actgatgcac aagatgctcg acacgaagat ggtcacgttc
     3241 accctcaacg ggcaaacggt cacggcgccc gcgaaccaga ccatcctcga gaccgccgac
     3301 gaccacggca ttgacgtacc gcgcctctgc tacatggagg gcatgcgcgc cgacggcaac
     3361 tgccgcacgt gcatggtgga gatcaagggc gagcgggtgc tcgcggcctc ctgctgccgc
     3421 tatccgaagg acgggatgga ggtcacgacg gacagcgccc gcgccgtgat cagccagaag
     3481 atgtcgatcg aactgctgct ctcggacatc cccgagacgt cctacacgct cgactcggag
     3541 ctcgatctgt gggcgaagaa gatgggcgtt ggcaaacccc ggttcggcgc ccgtccgcag
     3601 cccgtcgaag atctctcgca cccggccatg gcggtccacc tcgacgcctg cattcagtgc
     3661 aagcgctgcg tgcgcgcgtg ccgagaggag caggtgaacg acgtcatcgg gtacgcgttc
     3721 cgcggcggcc gctcgaagat cgtgttcgat ctcgacgacc cgatgggcga ctcgacgtgt
     3781 gtggcctgcg gcgagtgcgt ccaggcgtgc cccaccggcg cattgatgcc ggcgcgcgac
     3841 gtggcgagga tcgttcccga caagacggtg gactcggtct gcccgtactg cggcgtgggc
     3901 tgccagctca cctacaacgt gaaggacaac cggatcctgt acgtgcaggg ccgggacggc
     3961 cccgccaact cgagccggct gtgcgtgaag gggcgctacg gcttcgatta cgtccagcac
     4021 cgccatcggc tcaccaggcc gctgattcgc aagaagggcg tgccgaagca caaggacttc
     4081 acggtcgatc ccgagaagtg gcacgaggtg ttccgcgagg tgacgtggga cgaggcgctc
     4141 gacttcgccg cgaaggggct gcgcgagatc cgcgacacgc acgggaagcg cgcgctcgcc
     4201 ggcttcggct cggcaaaggg gaccaacgaa gaggcgtacc tcttccagaa gctcgtgcgc
     4261 accggcttcg gatccaacaa cgtcgaccac tgcacgcgcc tctgtcatgc gtcgagcgtc
     4321 gctgcgctca tggaagggat caactcgggc gccgtgtcca accaggtgcg cgacgtggcg
     4381 aacgccgagg tgatcttcgt catcggcgcg aacccgacag tcaaccaccc agtggccgcg
     4441 acctggatca agaacgccgc gaaagcgggc gccaggctga tcgtcgccga tccgcggcgg
     4501 ggcgatctcg cccgccacgc cacctacaac ctgcagttca acgccgatac cgacgtcgcc
     4561 ctcctcaacg cgatgctcca cgtcatcgtc gaggaaggcc tcgtggacga ggcgttcata
     4621 cgcgaccgga cctcggggta tgccgagctg gccgagaacg tgaagaagtt ctcgcccgag
     4681 gcga
//
LOCUS       DBNGSH010000502         6333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3104350, whole genome shotgun sequence.
ACCESSION   DBNGSH010000502 DBNGSH010000000
VERSION     DBNGSH010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3104350"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..545
                     /locus_tag="AC16CY_11565"
     CDS             357..545
                     /locus_tag="AC16CY_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR3354475.1"
                     /translation="MNFIARLRALLRDTSGQDLLEYALLVALIALVCVAVITSTGTGV
                     QTIFQRISTALTTAAPAP"
     gene            590..778
                     /locus_tag="AC16CY_11570"
     CDS             590..778
                     /locus_tag="AC16CY_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR3354476.1"
                     /translation="MNFIARLRALLRGTSGQDLLEYALLVALIALVCVAVITSTGTSV
                     QSIFQAISTALTTAAPAP"
     gene            889..1086
                     /locus_tag="AC16CY_11575"
     CDS             889..1086
                     /locus_tag="AC16CY_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR3354477.1"
                     /translation="MMRQAANTLVTWSILADESGQDLLEYALLVALIALVAVGAVTAV
                     GTNINGVFWTYISGVIGAVSP"
     gene            1070..1579
                     /locus_tag="AC16CY_11580"
     CDS             1070..1579
                     /locus_tag="AC16CY_11580"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HIR3354478.1"
                     /translation="MLSAPEILALTIVGTGTGLAAVIDLRTRRIPNALTAALAVIGIG
                     IAAAKLGPIGLGGALLGCALGLAFMLPGHFFGATGAGDVKLFAAAGALLGPATTVQAF
                     LYTAIVGGILAVAIAIRRRRLGRTLGATARLVTDGSAGAAAIESPEADNRFAYAPAIA
                     VGVVMAALL"
     gene            1597..2043
                     /locus_tag="AC16CY_11585"
     CDS             1597..2043
                     /locus_tag="AC16CY_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR3354479.1"
                     /translation="MQTRLRRMRQERGAALLEMALTLPLLLLVCVGILEFGRAYQTWQ
                     VMTNAAREGARIAVLPGTTNTDVTNRVQTYLTNGQLPNAASATVNIDRTQTISIGTST
                     APAAQVTVNYPFDFIVLDPIARLATGQGSATPSTLNMVAQALMRTE"
     gene            2074..2910
                     /gene="cpaB"
                     /locus_tag="AC16CY_11590"
     CDS             2074..2910
                     /gene="cpaB"
                     /locus_tag="AC16CY_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HIR3354480.1"
                     /translation="MRVFIVLALAIAAGGTFAFGTYRYVQNVQPTTVGVPTSGVVVAA
                     ADLQLGAELRSEDLRVIQWPTGAQPAGAFSTPAELVGRGLVQPIAMNEPFLPSKLAPK
                     EAGAGLPPVIAPGFRALSVRVNDVVGVAGYVLPGTRVDVVATVNPTQQPTDVTSKVIL
                     TNVLVLAAGTKIERDVEGNKPIAVSVVTLLVNPDAAEKLTLASTEGKIQLALRNPMDQ
                     TAPATPGIRPAALLGASAAAPVRRVATSAAPRTESRPAPAFPTVEIIRGDKRAQEVVA
                     QE"
     gene            2915..5029
                     /locus_tag="AC16CY_11595"
     CDS             2915..5029
                     /locus_tag="AC16CY_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HIR3354481.1"
                     /translation="MRMTSQTARIASLGVHMFIIGVFGTHLALAQVPGARPEGTVAAA
                     MRPGSAPAAENESPRAVQLLVGRSTLVDVGSPIARVSLTSADVADALVTGPTQLLVHG
                     KLPGAISMFVWDRSGAITRYEIAVQRDLDRLNTQVSQLFPGERIQVHSNGKSVVLSGL
                     VSSKDVADKAVAVAAGYVDNKADVVTLLQIQPGQRSNQVLLRVRFAEVSRTAMSEWGM
                     SIFTSATGVNNTIGRSTTQQFAAPGYQDLAWTKAGSGFGDDVTSASGKLVFSDFLNIF
                     LLNQQYDLGILIKALQQRGLFQSLAEPNLIAESGKEASFLAGGEFPIPVAQGSAANMA
                     VSVQFKEFGIRLNFTPTVSGDRVHLKVKPEVSTLDYGNAVVLNGFRIPALSTRRTETE
                     IELNNRQTFAIAGLMNNQVSQTMQKIPGLGDIPILGYLFKSKAAQRDQSELVVMITPE
                     ILPNNSPGVTPSLPNYPEQFLPPLSSEKARPAPAPAFTTGTRSQASAPAAAPAAANAR
                     NDIERAQDRAQQALEGGTSQPVAAQPITSAPVVAAQAPVQAVAPLAAQPVVPQPQARA
                     EQSEDQRQLDEAAREQAERDAKAAEKARKDAAKLAREQAERDAVAAREQAKRDAQAAR
                     EQAKRDEAAAKEQAKRDAVAAREKAKADQLAQEEAKRQAEVARREAAREQERQQAIEA
                     AAERLRAAEAEYQAELARRSTR"
     gene            5059..6204
                     /locus_tag="AC16CY_11600"
     CDS             5059..6204
                     /locus_tag="AC16CY_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR3354482.1"
                     /translation="MRIRLRQLRRDERGMSFVFVGLGFMAFFAATTLAIDVGMFMTAR
                     SQAQNAADAGALAGATALVFNDFNDRTPGGPAVQSAINTAMANAVMGSAASVQPGDVT
                     FPLGPTGANDRVKVEVFRTSTRSNPVTTLIGPIFGINNVDIWATATAEAAPANAMTCV
                     KPFTIPDRWEENSVPPNNTFDRYDNHGNVLPNADVYYPPTSPQYMGWNAENDKGTTMT
                     IRAGTGNNIEPSFYFSWKMPGASIGANFYEENITGCNPTVISRPYTMTQEPGNMMGPT
                     TTGIDDLIAQDPNAYYDSAAGKVVSSMSPSPRVFPIPMFDPDQYQAGITNGRNATLEM
                     VNWVGFFLLRRSGNNVEGIIVPITGVIDPNAGPAPAGSFPKAIRLVQ"
BASE COUNT         1106 a         2102 c         2114 g         1011 t
ORIGIN      
        1 cgctgacgcc gatgacgcgg tgtgcagtct cgtcctgtgt gtcgatcccg cgtgcgcttc
       61 gtgtcgatcc gggacacgcc gctggagcca cggaatcgac cggttcgtta cgaacctgac
      121 gactcgaacg tcgtgccgcg ggccgccgcg ggcggccagt ccgccgtccg gcacgacctc
      181 caagtcgctg ccgcatcagc tacttcgact cgaatggacg gttatcagtg gctggcctgg
      241 catgcccgtt gcgcaatgac ccgtgtggtc gcggtcatgc cccctcgaag tggccgcttt
      301 cggaacattg gcgtgtgcga tcgggaaacc accgatcgaa ccaaggaagg gatgtcatga
      361 acttcattgc tcgtttgcgt gcgctgcttc gcgacacttc tggtcaggac ttgctggaat
      421 acgcgctcct cgttgcgctg attgcactgg tgtgcgtggc ggtcatcacg tcgaccggca
      481 cgggcgtcca gacgatcttc cagcgcattt cgacggcact gacgacggcg gcgccggctc
      541 cgtaagtggc tggtagtcag cagatcgagt tgctaaagga agggatgtca tgaacttcat
      601 tgctcgtttg cgtgccctgc tgcgcggaac gtcaggtcag gacctcctgg agtacgcact
      661 cctcgtggcg ctgattgcgc tggtgtgcgt ggcggtcatc acgtcaaccg gcacgagtgt
      721 tcagtcgatc tttcaggcca tctcgactgc cctgacgact gcggcgccgg ctccgtaaca
      781 agggcctgca ctcagctgac caacgggagg gcggaccttc gggtccgccc tcttctaatc
      841 gtcgacgcgg ttcggaaggg ttcggttaca caacagagca cgacgaacat gatgcgacag
      901 gcagccaaca cactcgtgac gtggtcgatt ctggcggacg agtccggcca ggacttgctc
      961 gagtacgcat tactcgtcgc actgatcgcg ctcgtcgccg tcggcgcggt gacggcagtg
     1021 ggcaccaata tcaacggcgt gttctggaca tacatctcag gagtgatcgg tgctgtcagc
     1081 ccctgaaatc ctcgcgctca cgatcgtcgg cacgggcacc gggctcgccg ccgtcatcga
     1141 tctgcgcact cggcgcatcc cgaatgccct gactgcggcg ctggccgtga tcggcatcgg
     1201 catcgcggcg gccaagctcg ggccgattgg tctgggcggc gccctgctgg ggtgcgcgct
     1261 ggggctcgcc ttcatgctgc cgggacactt tttcggcgca acaggcgcgg gcgacgtaaa
     1321 gctttttgcg gcggccgggg ccctgctggg cccggccacg accgttcagg cgtttctcta
     1381 caccgcaatc gtcggaggca ttctggccgt ggcgatcgcc atccggcgcc ggcgtctcgg
     1441 gcgcacgctt ggcgcgacag ctcgcctggt caccgacgga tccgccggtg ccgcggcgat
     1501 tgaatctccc gaggctgata accgcttcgc ctacgcgccg gcgattgccg tcggcgtcgt
     1561 gatggcggcg ctgctgtgaa ggcgagggac gcacacgtgc agacacgact tcgacggatg
     1621 cgtcaggaac ggggcgcggc gcttctcgag atggcgctca cgctgccgct gttgctcctc
     1681 gtctgcgtcg gcatcctcga gttcggccgc gcgtaccaga cgtggcaggt gatgaccaat
     1741 gccgcccgcg aaggcgcgcg gattgccgtg ctgccgggca ccacgaacac cgacgtgacc
     1801 aaccgcgtgc agacctactt gaccaacggc cagctgccga acgccgcgtc cgcgaccgtg
     1861 aacatcgaca ggacccagac gatctcgatc ggcaccagta cggcccccgc tgcacaggta
     1921 accgtgaact acccgttcga tttcatcgtg ctcgacccga tcgccaggct ggcgacaggt
     1981 caggggagcg ccacaccctc gacgttgaac atggtcgccc aggccttgat gcgtactgaa
     2041 taggaaacag ggagcttgct gcttatggct cgcatgcgcg tatttatcgt ccttgccctg
     2101 gcaatcgccg ccggcggcac cttcgcgttc ggcacgtatc gatacgtgca gaacgtgcaa
     2161 ccgaccacgg tcggcgtgcc gacgtccggg gtggtcgtcg ccgcagccga tctgcagctc
     2221 ggcgccgagc tccgcagcga ggacctgcgc gtcatccagt ggccgaccgg cgcccagccc
     2281 gccggggcgt tctcgacgcc ggccgagctc gtcggccgcg gcctcgttca accgatcgcg
     2341 atgaacgagc cgttcctgcc gagcaagctg gcgccgaaag aagccggcgc cggattgccg
     2401 ccggtgattg ccccagggtt ccgcgcgttg tcggtccggg tcaacgacgt ggtgggcgtg
     2461 gctggctacg tgctgccggg cacgcgcgtc gacgtcgtgg cgaccgtcaa cccgacacag
     2521 cagccgaccg acgtcacgtc gaaggtgatt ctgaccaacg tgctcgtgct cgccgcgggc
     2581 accaagatcg agcgcgacgt cgaaggcaac aagccgattg cggtcagcgt ggtcacgctt
     2641 ctggtcaatc cggatgcggc cgagaagctg acgcttgcca gcaccgaagg aaagattcag
     2701 ctggcgcttc gcaaccccat ggatcaaacg gctccggcga cgccgggcat caggcccgcc
     2761 gcgctgctcg gtgcgtcggc cgctgcgccc gtccgccggg tcgcgaccag cgccgctccc
     2821 cgtacggaga gccgaccggc accagcgttt ccgacggttg aaatcatccg gggcgacaaa
     2881 cgggcacaag aagttgtggc ccaggagtag cgagatgcgt atgacatcgc agacggcacg
     2941 gatcgcctcg ttgggcgtcc acatgttcat catcggcgtg ttcggcaccc acctggcgct
     3001 cgcgcaggtg ccgggggccc gacccgaggg gaccgtcgcg gccgcgatgc ggcccggctc
     3061 ggcgccagcc gccgagaacg agagcccgcg ggcagtgcag ctgctcgtcg gacgctcgac
     3121 gctggttgac gtcggctcgc cgatcgcgcg cgtgtcgctg acgagcgccg acgtggcgga
     3181 cgcgctcgtg accggcccca cccagctgct cgtgcacggc aaactgccgg gcgcgatctc
     3241 gatgttcgtg tgggatcgca gcggcgccat cacacggtac gaaatcgccg tgcagcgcga
     3301 cctcgaccgc ctgaacaccc aggtgagtca gctgttcccc ggtgaacgca tccaggtcca
     3361 cagcaacggc aagagcgtcg tgctttcggg actggtgtcg agcaaggacg tcgccgacaa
     3421 ggctgttgcc gtcgccgccg gctacgtcga caacaaggct gacgtcgtga cgctcctgca
     3481 gatccagccg ggacagcgca gcaaccaggt gctgctgcgc gtgcggttcg cagaggtcag
     3541 ccggaccgcg atgtcggaat ggggcatgtc catcttcacc agcgcgaccg gcgtcaacaa
     3601 cacgatcggc cggtcgacga cgcagcagtt cgcggcgccc ggctaccagg atctcgcgtg
     3661 gaccaaggcg ggctccggct tcggagacga cgtgacgagc gcgtcaggaa agctcgtgtt
     3721 cagcgacttc ctgaacatct tcctgttgaa ccagcagtac gaccttggca tcctgatcaa
     3781 ggcgctccag cagcgcgggc tcttccagag cctggccgag ccgaacctca ttgccgagag
     3841 cggtaaggag gcgagcttcc tggcgggcgg cgagttcccg attccggtgg cgcagggctc
     3901 ggcggccaac atggccgtgt cggtccagtt caaggagttc ggcatccgcc tgaacttcac
     3961 gccgacggtg agcggcgatc gcgtgcacct gaaggtcaag cccgaagtca gcacgctgga
     4021 ctacggaaat gccgtggtgc tgaacggctt ccgcattccc gccctgtcca cgcggcggac
     4081 cgagaccgag atcgagctga acaaccggca gacgttcgcg atcgcgggcc tcatgaacaa
     4141 ccaggtgtcg cagacgatgc agaagattcc gggcctcggg gacatcccga tcctcggcta
     4201 tctgttcaag agcaaggccg cgcagcggga ccagtccgag ctcgtggtga tgatcacccc
     4261 ggagatcctg ccgaacaact cgccgggcgt gaccccgtcg ctgccgaact atccggagca
     4321 gttcctgccg ccgctctctt cggagaaggc gcgtccggca cctgcgccgg cgttcacgac
     4381 gggcacgcgc tcgcaggcga gcgctccggc ggcagcgccc gccgcggcga acgcgcgcaa
     4441 cgacatcgag cgcgcgcagg atcgggcgca gcaggcgctc gaaggtggga cgtcacagcc
     4501 cgtggccgcc cagccgatca cctcggcccc ggtggttgcg gcgcaggctc cggtgcaggc
     4561 cgtcgctccg ctggcggcgc agcccgttgt cccgcagccc caggccagag cggaacagtc
     4621 cgaggatcag cggcagctcg acgaggccgc gcgcgaacaa gccgagcgcg atgccaaggc
     4681 tgccgagaag gcgcggaagg acgccgcgaa gctcgcgcga gagcaggccg agcgcgatgc
     4741 cgtcgcggcc agagagcagg ccaagcgtga tgcgcaggcg gccagggagc aggccaagcg
     4801 cgatgaggcc gccgcgaagg aacaggccaa gcgggacgcc gtggctgcca gggagaaggc
     4861 gaaggccgac cagctcgccc aggaagaagc gaagcggcag gcagaggttg ccaggcgtga
     4921 ggccgcgcgc gagcaggagc gtcagcaggc catcgaggcg gcggccgagc ggctgcgcgc
     4981 ggccgaggct gaataccagg ccgagctggc tcgtcggtcc acacggtaag cgcggcgtcc
     5041 ggagaaaggg agcacgttat gcgaatccgt cttcgccagc tgcggcggga cgagcgcggc
     5101 atgtcgttcg tgttcgtcgg gctcggcttc atggcgtttt ttgcggccac gacgctagcg
     5161 atcgacgtcg gcatgttcat gaccgcccgc tcgcaggcgc agaacgccgc cgatgcgggc
     5221 gccctcgccg gcgcgacggc ccttgtcttt aacgacttca acgaccgaac cccggggggg
     5281 ccggccgttc agagcgccat caacacggcg atggcgaatg ccgtcatggg aagcgccgcg
     5341 tccgtgcagc ctggcgacgt gacgtttccg ctcgggccga ccggtgcgaa tgaccgggtg
     5401 aaggtcgagg tcttccgcac gtcgactcgc agcaatcccg tcacgaccct gatcggcccg
     5461 atcttcggca tcaacaacgt ggacatctgg gccacggcga ccgccgaggc ggctcccgcc
     5521 aacgcgatga cgtgtgtcaa gccgttcacg atccccgacc ggtgggagga gaactccgtt
     5581 ccgccgaaca acaccttcga caggtatgac aaccacggaa atgttctgcc gaatgccgac
     5641 gtctactacc cgcccacgtc ccctcagtac atggggtgga acgcggaaaa cgacaagggc
     5701 accacgatga cgatccgggc gggcaccggc aacaacatcg agccgtcgtt ctacttctcg
     5761 tggaagatgc ccggcgcgag catcggcgcc aatttctacg aggagaacat caccggttgc
     5821 aacccgacgg tgatcagccg tccctacacg atgacgcagg aaccgggcaa catgatgggc
     5881 ccgacgacga cgggcatcga cgatctcatc gcgcaggatc ccaacgcgta ctacgactcg
     5941 gccgcaggca aggtggtcag ctccatgagt ccgagcccgc gcgtgtttcc tattccgatg
     6001 ttcgatcccg atcagtatca ggccgggatt acgaacgggc gaaacgcgac cctcgagatg
     6061 gtgaactggg tcggcttctt cctgctgcgc cggtccggga acaacgtcga agggatcatt
     6121 gtcccgatta cgggcgtcat cgatccgaac gcgggacccg cgcctgcggg ttcgtttccc
     6181 aaggctatcc gattggtgca atagaccatg gcccgactca caggctccct ctacaccccc
     6241 gacgagacgt ttcgaaccga cgtcgccacg atgctgcggc tcaccgcggt gcaggtgtcg
     6301 ctcggcgacg atcgagcgcc gcgccagggc ggc
//
LOCUS       DBNGSH010000503         2876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_217225, whole genome shotgun sequence.
ACCESSION   DBNGSH010000503 DBNGSH010000000
VERSION     DBNGSH010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_217225"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="AC16CY_11605"
     CDS             complement(<1..1311)
                     /locus_tag="AC16CY_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HIR3354483.1"
                     /translation="MNPLDASDWFAARHIGPSAEDLAGMLQAVGASSLDALVGEAIPD
                     SIRLRRPLSLPPGESEHQYLERLQRLSEGNKVFRSYIGLGYHDTITPSVILRMVMENP
                     GWYTPYTPYQSEIAQGRLESLLNFQTMVTDLTGMEVANASLLDEATAAAEAMALMRRV
                     YTSKPAAGQRPVCLVSDRCLPQTIDVLHSRAEPLGIALDVVPIDRMTFDGHAFGAIVQ
                     YPDARGELYDLAPLVDRAHKAGALVAVGADLLSLALVTPPGEMGADVVFGNAQRFGVP
                     LGYGGPHAAFFAVRQAFVRQLPGRLVGVSVDVHGHQAYRMTLATREQHIRRERATSNI
                     CTAQVLLANMAAMYAVYHGPQGLRAIAARVHTLARVLEAELAALGVKQLNANYFDTLR
                     LEVSAGAEAVARLARAAKMNFHAVDARTVSVSLNETTSASDVRDI"
     gene            complement(1311..1688)
                     /gene="gcvH"
                     /locus_tag="AC16CY_11610"
     CDS             complement(1311..1688)
                     /gene="gcvH"
                     /locus_tag="AC16CY_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR3354484.1"
                     /translation="MAYPADRKYTKDHEWIRISGSDAEIGITDFAQEQLGDVVYVELP
                     EPGRTITAGESFGSIESVKAVSELFAPMSGTVVAVNDALKDHPETVNAKPHDTWMVKI
                     RLTDPKAAGTLLDSAQYEAVIQA"
     gene            complement(1711..2823)
                     /gene="gcvT"
                     /locus_tag="AC16CY_11615"
     CDS             complement(1711..2823)
                     /gene="gcvT"
                     /locus_tag="AC16CY_11615"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR3354485.1"
                     /translation="MTSTTTELRATPLRARHVASGARMVPYAGWEMPVEYSGITNEHL
                     AVRTRAGLFDVSHMGEIEVAGKGALEAVQKISSNDAGALKVGQAHYAALTSPQGTFLD
                     DMLVYRMAPSHFMLVVNAANTASDFEWIGEQVKAAGDAAVVNSSSRYALIAIQGPAAR
                     EVLQPLTGVDLGELRYYWFGHGEVAAARVTISRTGYTGEDGYEIFVPPAMADRVWQAL
                     LESGRPADVVPCGLGARDTLRLEAAMRLHGNDIDQSTTVLEADLGWTIAWNKGDFVGR
                     QALLEQKEKGLTRKLVGFEMIDPGIARHGYAVMSSGAKVGEVTSGTQTPFLKKAIGMA
                     YVPVALATQGTELSIDIRSRASKARVVPLPFYRRKP"
BASE COUNT          459 a          954 c          965 g          498 t
ORIGIN      
        1 gatatcgcgg acatcggagg cgctcgtcgt ctcgttgaga gagacggaga ccgtgcgggc
       61 gtcgacagcg tggaagttca tctttgccgc acgcgccagg cgcgcgaccg cctcggcgcc
      121 ggcggacacc tccagccgca gcgtgtcgaa gtagttcgcg ttgagctgtt tcacgccgag
      181 cgctgcaagc tccgcttcga ggactcgcgc cagcgtgtgg accctggcgg cgattgcgcg
      241 cagcccctgc ggaccgtgat agacggcgta catcgccgcc atgttggcca gcagcacctg
      301 cgccgtgcag atgttcgacg tcgccctttc gcggcggatg tgctgctcgc gcgtggccag
      361 cgtcatccgg taggcctgat gtccgtggac gtccaccgac acgccgacga ggcggccggg
      421 caactgccgg acgaatgcct ggcgcaccgc gaagaacgcc gcgtgcggcc cgccgtaccc
      481 gagtggcacg ccaaaccgct gcgcgttccc gaagacgacg tcggcgccca tctctcccgg
      541 gggcgtgacg agcgccagcg acaacagatc ggccccgacg gcgacgagcg ccccagcctt
      601 gtgagcgcgg tcaacgagcg gcgccagatc gtacagttcg ccgcgcgcgt ccgggtactg
      661 gacgatggcg ccgaatgcgt gtccgtcgaa cgtcatccga tcgatcggga cgacgtcgag
      721 cgcaatcccg agcggctcgg ccctcgagtg gagcacgtcg atcgtctgcg gaaggcagcg
      781 gtcgctgacg aggcacacag ggcgctggcc tgcggctggt ttcgacgtat acacccgtcg
      841 catcagggcc atcgcttcgg ccgccgccgt cgcctcgtcg agaagtgagg cgttggccac
      901 ctccatcccc gtcagatcgg tcaccatcgt ctggaagttg aggagcgatt cgagccggcc
      961 ttgcgcgatc tcggactgat agggcgtgta tggcgtgtac cagccggggt tctccatcac
     1021 catgcgtagg atcacgctcg gcgtgatggt gtcgtggtag ccgaggccga tgtaggacct
     1081 gaagaccttg ttgccctctg acaggcgctg caggcgctcg aggtactggt gctcgctctc
     1141 gccgggtgga aggctgagag gcctgcgcag gcggatggaa tcggggatcg cctcgccgac
     1201 gagggcgtcg agtgacgagg ccccgaccgc ctggagcatg cccgcgagat cttctgcgga
     1261 cggtccaata tggcgggcgg caaaccagtc cgacgcgtcg agcgggttca tcaggcctgg
     1321 atcacggctt cgtactgggc gctgtcgagc agcgtgccgg cggccttcgg gtccgtgagg
     1381 cgaatcttca ccatccaggt gtcgtgcggc tttgcgttca cagtctccgg gtggtccttg
     1441 agcgcgtcat tgacggcaac gaccgtaccg ctcatgggcg cgaagagctc ggagaccgcc
     1501 ttgaccgact cgatcgagcc gaacgactcc cccgccgtga tcgtccgacc cggttcggga
     1561 agctcgacgt agacgacgtc gccgagctgc tcctgggcga agtcagtgat gccgatctcg
     1621 gcgtcgctgc ccgagatacg aatccactcg tggtccttgg tgtacttgcg atctgccgga
     1681 tacgccatca ataaatgact cccgacccca ttacggtttt cgtctgtaga acggcagcgg
     1741 caccacgcgc gccttcgacg cgcggctgcg gatgtcgata ctcagctcag ttccctgggt
     1801 ggccaacgct acgggcacgt acgccatccc gatcgccttc ttcaggaacg gcgtctgcgt
     1861 gccactcgtc acctcaccga ccttcgctcc gctcgacatg acagcgtagc cgtggcgggc
     1921 gatgcccggg tcaatcatct cgaagcccac gagcttgcgc gtcagcccct tttccttctg
     1981 ctccaataga gcctggcggc cgacgaagtc gcccttgttc cacgcgatcg tccagccaag
     2041 atcggcttcg aggacggtgg tcgattgatc gatgtcgttc ccgtgaagcc gcatggcggc
     2101 ttcgagccgg agcgtgtcgc gggcgccaag gccgcagggg acgacatccg ccggccggcc
     2161 tgactcgagg agcgcctgcc acacgcggtc ggccatcgcg gggggtacga agatctcgta
     2221 gccgtcctcg cccgtatagc cggttcggga aatcgtgacg cgcgccgccg ccacctcgcc
     2281 gtgcccgaac cagtagtagc ggagctcgcc gaggtcaaca cccgtaagcg gctgaagcac
     2341 ttcgcgcgcg gcaggtccct ggatcgcaat cagcgcgtag cggctgctcg aattcacgac
     2401 cgccgcgtcg cccgcggcct tcacctgctc gccgatccac tcgaagtcgc tggcggtgtt
     2461 ggccgcgttg acgacgagca tgaagtggct gggcgccatg cggtagacca gcatgtcgtc
     2521 gaggaaggtg ccctgcggag acgtcagcgc cgcatagtgt gcctgcccga ccttcagcgc
     2581 cccggcgtcg ttgctcgaga tcttctgcac ggcctcgagg gcgcccttgc cggccacttc
     2641 gatctcaccc atgtggctga cgtcgaagag gcccgcgcgg gtgcgcacag cgagatgctc
     2701 gttggtgatc ccggagtact cgacgggcat ctcccagccc gcgtaaggga ccatgcgggc
     2761 ccctgaggcg acatggcgtg cgcgcagcgg cgtggcgcgg agttctgtag tcgtactggt
     2821 catgaagccg cgcgtaaagc ggtggggccc aacgccttga ctgtactcgt cgcgcg
//
LOCUS       DBNGSH010000504         2380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2424804, whole genome shotgun sequence.
ACCESSION   DBNGSH010000504 DBNGSH010000000
VERSION     DBNGSH010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2424804"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="AC16CY_11620"
     CDS             complement(<1..590)
                     /locus_tag="AC16CY_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354486.1"
                     /translation="MIGPMARIEEQPDLKETLQGLEKTMAKQRQLLERLAAYAARREW
                     RGLLAGITNPLRLEHSGRKVYSQNDEDGIIEEIFRRIGIAPARGIFIEFGAGHGLENN
                     THYLLRKGYAGVWLEASAELVERALRSFRTYVARDQLQIERVTVTAEIIDDHLSRMAR
                     GRDVALLAIDLDGNDYWVWKAIRSFSAAVVAIEYNA"
     gene            644..1630
                     /locus_tag="AC16CY_11625"
     CDS             644..1630
                     /locus_tag="AC16CY_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354487.1"
                     /translation="MIRARALAIAACVSAAGPALAQQGPVASMFRDDQQRGQGTGLSF
                     ATLEMAPTAAANFPAQIYLTAGDLERAFDRPQFRPDGAIVPTNTELAITAAAPATQRV
                     LIDRVRRSSGVMRDLQDQIEARRKQAKPASGGQAGLLEIGVDTFTAQLPRNAGSKQSG
                     AFPRAVCLIATDFPKGGAIDRRELFAQDRVRKGVAACLDSLDASGVQSLVLPLMGAAS
                     SGTQAKDPRYEGQNILKECRLLNSVAGIALGIHDFAPGRRRIREIGIVQWDQEIREMF
                     SLQQGTRLSQTAQNTYRIYAEQIASAFRRGLAGEKTTSSDIDGSCTAILSAQ"
     gene            1676..>2380
                     /locus_tag="AC16CY_11630"
     CDS             1676..>2380
                     /locus_tag="AC16CY_11630"
                     /inference="COORDINATES: protein motif:HMM:NF023321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HIR3354488.1"
                     /translation="MRIPLIRALGWFVLANAVLFKAISAVFWTSVVESPESASAGLYR
                     TLANVVHWPMLAGLLVGLPLLTLIALWPRRRWVLVAAAVIGGLATFFLIVNAVVFAQY
                     RTHLGPWVWGLLFGGSGRETFLFSTLMRAIIVALFAGGLALQAGLFWSCARVADRWSG
                     RRYGARLWAAWTAAFVLVNGWHVWADATYRADITQENGNLPYFYPLTMKRDLHAWGLV
                     DRVEAHEAAAPVTGAGT"
BASE COUNT          361 a          800 c          807 g          412 t
ORIGIN      
        1 ctggcgttgt actcgatggc gacgacagcg gcgctgaacg atcgaatggc cttccagacc
       61 cagtaatcgt tgccatccag gtcgatcgcg agcagcgcga cgtcgcgtcc gcgagccatc
      121 cgcgagagat ggtcgtcgat gatttcggcc gtcacggtga cgcgctcgat ctggagctgg
      181 tcgcgcgcga catacgtccg gaacgaacga agcgctcgct cgaccagttc ggcggacgcc
      241 tcgagccaca cgccggcgta ccctttgcgc agcaggtagt gcgtgttgtt ctcgaggccg
      301 tgtccggcgc cgaactcgat gaagatgccg cgcgcgggtg cgatgccgat gcggcggaag
      361 atctcctcga tgatgccgtc ttcgtcattc tggctgtaca ccttgcggcc cgagtgctcg
      421 agccggaggg gattggtgat gccggcgagg aggccgcgcc actcccggcg cgcggcgtag
      481 gcggcgagcc gctcgagcag ctggcgctgt ttcgccatgg tcttctcgag cccctgcagc
      541 gtctctttca ggtccggttg ctcttcaatc ctcgccatcg ggccgatcat aggtcaaagc
      601 tcaaaggtca aaggtaagat gccttatggt tcaacgacga cttttgattc gcgcacgcgc
      661 gctcgccatc gcggcgtgcg tgtcggcggc cggcccggcg ctggcgcagc aggggcccgt
      721 cgcctccatg ttccgcgacg atcaacagcg gggacagggt accgggctca gcttcgcgac
      781 cctggagatg gcgccgaccg cggcggcaaa tttccccgcg cagatctatc tcaccgccgg
      841 cgacctcgag cgtgcgttcg acaggcctca attccggccc gacggcgcca tcgtgccgac
      901 caacaccgaa ctggcgatca cggcggcggc tcccgcaaca cagcgggtgc tcatcgaccg
      961 cgtccggcga agctcgggcg tgatgcgcga cctgcaggat cagatcgagg cgcggcggaa
     1021 acaggccaag cccgcctccg gcggtcaggc cggcctgctc gagatcggcg tggacacgtt
     1081 cacggcgcag ttgccccgca acgccggcag caagcagagc ggcgcgtttc cgagggccgt
     1141 ctgcctgatt gccacggact tccccaaggg cggcgcgatc gaccgccggg aattgttcgc
     1201 tcaggatcgc gttcgcaagg gcgtcgccgc gtgtctcgac tcgctcgacg cctccggcgt
     1261 tcagtccctc gtcttgccgc tgatgggcgc ggcctcgtcc ggcacgcagg ccaaggaccc
     1321 gcgctacgag ggccagaaca tcctcaagga gtgccgcctg ctgaactccg tcgcaggaat
     1381 cgcgttgggg attcacgatt tcgcgccggg gcgccgacgc atccgtgaga tcggcatcgt
     1441 tcagtgggac caggaaatca gggagatgtt cagcctccaa cagggaacgc gcctctcgca
     1501 gacggcgcag aacacctatc gaatctacgc ggaacagatc gcctcggcgt tccgcagagg
     1561 gcttgccggc gaaaagacga catcgagtga catcgacggc agctgcaccg cgatcctcag
     1621 cgcccaataa gacaccgccc gcggtgccgg ttgctggcgg cctgtatcat tcggaatgcg
     1681 catcccgctg atccgcgcgc tcggctggtt cgtcctggcc aatgccgtgc tgttcaaggc
     1741 gatctcggcg gtgttctgga cgtcggtcgt cgagtccccc gagtcggcgt ccgcgggtct
     1801 ctatcgcacg ctggcgaacg tcgttcactg gcccatgctc gcgggcttgc tcgtcggcct
     1861 cccgctgctg acgctcatcg ccctgtggcc gaggcgccga tgggtcctcg tcgcggccgc
     1921 cgtgatcggc ggcctcgcca cgttcttcct gatcgtcaac gcggtcgtgt tcgcgcagta
     1981 ccggacgcac ctcggaccgt gggtgtgggg gctgctgttc ggcggctccg gccgggagac
     2041 gttcctcttc tcgacgctca tgcgcgcgat catcgtggcc ctcttcgccg gcggcctggc
     2101 gctccaggca ggcctgttct ggtcgtgcgc gagagtcgcc gaccgctgga gcggccggcg
     2161 ttacggcgcg cgcctgtggg ccgcctggac cgcggcattc gtgctggtga acgggtggca
     2221 cgtctgggcg gatgcgacct accgcgcgga catcacgcag gagaacggca acctcccgta
     2281 tttctatccg ctgacgatga agcgggacct gcacgcgtgg ggactggtgg accgcgtgga
     2341 ggcgcacgag gcggcggccc ccgtaacggg cgcgggcacc
//
LOCUS       DBNGSH010000505         2303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_48276, whole genome shotgun sequence.
ACCESSION   DBNGSH010000505 DBNGSH010000000
VERSION     DBNGSH010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_48276"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC16CY_11635"
     CDS             <1..252
                     /locus_tag="AC16CY_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3354489.1"
                     /translation="GPSLQDVNVPAHAPPLFIAVGSSHFNVTNGCLALFAAWKAAGKP
                     AEIHVYDQVSAGFGMTKRGLPVDSWNDRLLEWLMARKLR"
     gene            281..535
                     /locus_tag="AC16CY_11640"
     CDS             281..535
                     /locus_tag="AC16CY_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354490.1"
                     /translation="MREPKQERTFFWRSAPNSNRTQQAVRQGDWKLVVDANHLLLFNL
                     RDDIGERHDRTAQRPEIVGRLRTLLGDWARSVDADAKALK"
     gene            574..1596
                     /locus_tag="AC16CY_11645"
     CDS             574..1596
                     /locus_tag="AC16CY_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3354491.1"
                     /translation="MTVRHVSGLAVSAALVLGPGLVFSPVAAQNAAPAAATPARILPV
                     TANLRGAPPTGPHAVVIEHDQALATHTIYRPATLGSSKHPVLVWGEGGCAKDGLQFPE
                     FLNEIASHGVVVIADGPPVARTAPGGGAAPGPRGGGAAPPAAGAAPAPGARAGAGGQG
                     RGGVPRVMTDGTALIAAIDWIAKEGNDPASRFYQKVEASRVAAMGMSCGGLMSYGASA
                     DPRVATVGIWNSGLIQPDEKIFAGLHSPVIIITGGEKDIAYANGKRDFETMPSKIPVF
                     YGVFPSVGHGGTYNEDNGGAFGVAAVAWLKWQLLNDSGARTFFVGRDCRLCTDPQWQT
                     ASRSLE"
     gene            1630..>2303
                     /locus_tag="AC16CY_11650"
     CDS             1630..>2303
                     /locus_tag="AC16CY_11650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3354492.1"
                     /translation="MANLQTRISKVPLAALIGWTMCTVALLAQTTQTPTFPNAPPGFD
                     GPRQGIATGRVERVEYASSVTGGKKPAMVYTPAGYSSSQKYPVLYLLHGIGGNETHWP
                     GPGAAAAILDNLIADRKTVPMIVVMPHGRSSNEPETSLFGGRGGRGARGATPDAATGG
                     GQARSGAPPAAGDGREGGRGGASMAVEFQAYAAFERELLSDLIPFIESKYSVAADREH
                     RALAGLS"
BASE COUNT          380 a          762 c          800 g          361 t
ORIGIN      
        1 gggccctcgc tgcaggacgt caacgtgccc gcgcacgcgc cgccgctctt catcgcggtc
       61 ggttcgtccc acttcaatgt gaccaacggc tgcctggcgc tcttcgccgc gtggaaggcc
      121 gccggcaagc ccgcagaaat ccacgtctac gatcaggtca gcgccggctt cggaatgacg
      181 aaacgcgggc tgccggttga ttcctggaac gaccggctgc tcgagtggct catggcccgg
      241 aaactcagat gaccacacga acgctgattc gcgttgtgac gtgcgcgagc caaagcagga
      301 gcgaacgttc ttctggcggt cggcgcccaa ctccaaccgt acgcagcagg ccgtccgtca
      361 gggcgactgg aagctcgtgg ttgacgcgaa ccacctgctg ctcttcaatc tgcgcgacga
      421 catcggcgag cgtcacgatc gaacggcgca acggccggag atcgttggtc gtcttcgaac
      481 cctgctcggt gactgggcgc gcagcgtgga cgccgacgcg aaggccctga agtagaaggc
      541 agatactcga ccatgtagtg aggagacaac accatgacag tcagacacgt gagcggactt
      601 gccgtttcgg cggccctcgt tcttggcccg ggcctcgtct tcagcccggt tgccgcgcag
      661 aacgcggcac ccgcggccgc cacaccggcg cgcattctgc cggtgacggc caatctgcgg
      721 ggcgcgcccc cgacaggacc gcacgcggtc gtgatcgagc acgatcaggc gctcgccacg
      781 cacaccatct atcgccctgc gacgttgggg tcgtcgaagc atccggtcct ggtctggggc
      841 gagggcggct gtgcgaagga cggcctgcag tttcccgagt tcctcaacga gatcgcgtcg
      901 cacggcgtcg tcgtgatcgc cgatggccct ccggtcgcac gaacggcgcc gggtggcggt
      961 gcagcgcctg ggccgcgtgg gggcggggcg gctccgccgg cggctggcgc ggcgccggcc
     1021 cctggcgcca gagcgggtgc aggcggacag ggacgaggcg gtgtgccgcg cgtcatgacc
     1081 gacggcacgg cgttgatcgc ggcgatcgac tggatcgcca aagaaggcaa cgaccctgca
     1141 agccgcttct atcagaaggt cgaggccagc cgcgtggccg cgatggggat gtcgtgcggc
     1201 gggctcatgt cgtacggcgc ctcggccgat ccgcgcgtgg ccacggtcgg catctggaac
     1261 agcggcctga ttcagcccga cgagaagatc ttcgccggcc tgcactcgcc cgtgatcatc
     1321 atcaccggcg gcgagaagga catcgcctat gccaacggca aacgcgactt cgaaacgatg
     1381 ccctcgaaga ttcccgtgtt ctacggcgtg tttccgtccg tcggtcacgg cggcacgtac
     1441 aacgaggaca acgggggcgc cttcggcgtt gcggccgtcg cgtggctcaa atggcagctg
     1501 ctgaacgaca gcggggcccg cacgttcttc gtgggacgtg actgccggct ctgcaccgac
     1561 ccgcagtggc agaccgcgtc acgctcgctg gagtagagtg gggacgtcat ccaattgagg
     1621 agagtcgaga tggccaattt gcagacccga atctcgaagg taccgcttgc ggcgctcatc
     1681 ggctggacca tgtgcaccgt ggctctgctc gcacagacga cgcagacgcc gacgttcccg
     1741 aacgcgccgc cgggcttcga cggaccacgg cagggcatcg ccaccggtcg cgtcgaacgc
     1801 gtcgagtacg cctcgtcggt gaccggcggg aagaagccgg ccatggtgta caccccggcg
     1861 ggctattcct catcgcagaa gtacccggtg ctctacctgc tgcacgggat cggcggtaac
     1921 gagacgcatt ggccgggacc gggggccgcc gccgccatcc tcgacaacct gatcgccgat
     1981 cggaagacgg tgccgatgat cgtcgtgatg ccgcacggcc gctcctcgaa cgagccggag
     2041 acatcactct ttggtggacg cggtggtcga ggtgcgcgcg gtgctactcc cgacgcggct
     2101 acaggaggcg ggcaggctcg ctcgggagcg ccgcccgcgg ccggcgacgg tcgcgagggc
     2161 gggcgcggcg gcgcgtcgat ggcggtcgag ttccaggcgt acgcggcgtt cgagcgcgaa
     2221 ctgctctcgg atctgatccc gttcatcgag tcgaagtact cggtggcagc tgatcgcgag
     2281 caccgcgcgc tcgccggtct gtc
//
LOCUS       DBNGSH010000506         1839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1576346, whole genome shotgun sequence.
ACCESSION   DBNGSH010000506 DBNGSH010000000
VERSION     DBNGSH010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1576346"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..1000)
                     /locus_tag="AC16CY_11655"
     CDS             complement(311..1000)
                     /locus_tag="AC16CY_11655"
                     /inference="COORDINATES: protein motif:HMM:NF025699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HIR3354493.1"
                     /translation="MSPKRREPSILRGSSWLRDERGVALIITLLTMMLLSALGLALTL
                     TTSTEMRVSSAYAGSAEVFYAADAAFERSTQDLLLIPDWNLVLGGVVRSSLVDGVPGP
                     RQRPDGSVLDLQRETDRLNCGGATCGPWGANNPRWQLFAHAPLADVSPAQAINSRIYV
                     VVWVGDDATENDGQPLVDGDTTAGPNPGEGLIQLLVHAYGPGPTRREVEGTLKRQDAR
                     MRVLSWREVRQ"
     gene            complement(997..>1839)
                     /locus_tag="AC16CY_11660"
     CDS             complement(997..>1839)
                     /locus_tag="AC16CY_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354494.1"
                     /translation="TASQSTLASPLPPGALDLQLDAPPNCATPTINQLCGLGAGDRLL
                     IFDQRSQWDVFSVDQVGLGGAVLQHRGAPSAADFRAGAIAAHVRLGTYHLKSDDAAMV
                     YQLMRHDGWTTDLPVVDDVVALRFQYFGDPEPPRRTVTALDDPAGPWTTYGPAPPPVD
                     DIRGNWPAGENCAFFVVDRDHVPRLGTLVGGGLASVELTPAMLTDGPWCPDGLAPNRF
                     DADLFRVRKIRVTLRVQSALASLRGPAGVLFLKGGTARTGYRYVPDLEVQFDVTPRNM
                     NLDR"
BASE COUNT          337 a          586 c          618 g          298 t
ORIGIN      
        1 cgaaggtttt gtctcactgg ccgtggactc agcaatgacg ctgccattcg atcgtccaga
       61 ttcctggacg gctcgtaacg tgcttcgtca taaacacttg gaggtggaaa gtggcccgga
      121 cacggcccgg tatggagggg cctgtgcgcc agtttggaac actagatgac gttcttgcaa
      181 ccgcgaagtg cgacctgacc gcgccgccac agactgtccg gtcgctccca cgatctcggt
      241 cggagcggag tggaacgata gccccgccgg tgcgagggtg cgagagtgcg agggtacggg
      301 tagacgtgga ctactgacga acctctcgcc atgacagcac gcgcatgcgc gcgtcctggc
      361 gtttcagagt gccttctacc tcccggcggg tggggccagg gccgtaggcg tgcacgagaa
      421 gctggatgag gccttcacca gggtttgggc cagccgtcgt gtcgccatcg accagcggct
      481 ggccatcgtt ctcggttgcg tcgtccccaa cccacacgac cacgtagatt cgtgagttga
      541 tcgcctgggc cggactcacg tcggccagcg gcgcgtgagc gaacagctgc cagcgcgggt
      601 tgttcgcgcc ccacggcccg cacgtcgcgc caccgcagtt caaccgatcg gtctcgcgct
      661 gcaggtcgag gacggaaccg tcgggccgct ggcgcggccc cgggacaccg tcgacaagag
      721 acgagcgcac gactccgccg agcaccagat tccaatcggg aatcagcaac agatcctggg
      781 tcgagcgttc gaaggcggcg tcggcggcat agaacacttc ggcgctgccg gcatacgcgc
      841 tcgagactcg catttcggtc gacgtcgtca gcgtcagtgc caggcccagc gccgatagga
      901 gcatcatcgt caacagggtg atgatcaggg cgacgccgcg ctcgtcgcgc agccatgaag
      961 agccacgaag gattgacggc tcacgtctct tggggctcat cgatcgaggt tcatgttccg
     1021 cggcgtcaca tcgaactgca cttccaggtc cggcacgtag cggtatccag ttcgtgcggt
     1081 gccccctttg agaaacagca ctccggccgg gccgcgcagt gacgcgaggg ccgactgcac
     1141 gcgcagcgtc acgcggatct tgcggacgcg gaacagatcg gcgtcgaatc tgttcggcgc
     1201 gagcccgtcc ggacaccagg gaccgtctgt cagcatggcc ggtgtcaatt caacggaagc
     1261 aaggccaccg ccgacgaggg taccgaggcg tggcacatga tcacgatcga cgacgaaaaa
     1321 cgcgcagttc tcgcccgccg gccagttgcc tcgaatgtcg tcgacaggag gcggagccgg
     1381 tccataggtc gtccatggac ccgctggatc gtcgagggcc gtgaccgtac ggcgaggagg
     1441 ctcggggtcc ccgaagtact ggaaccgcag tgcaacgacg tcgtccacca cgggcagatc
     1501 agtggtccac ccgtcgtgcc gcatcaattg gtacaccatt gccgcgtcgt ccgacttgag
     1561 gtggtacgtc cccaaccgga cgtgcgcggc aattgcaccg gcgcggaaat ccgcggccga
     1621 gggagcgccg cggtgctgaa gcacggcgcc gcctagtcca acctggtcga cgctgaagac
     1681 gtcccattga ctgcgctggt cgaagatcaa gagccggtca ccggcgccca gcccgcacaa
     1741 ctggttgatc gttggagtcg cgcagttcgg cggcgcgtcg agctgcaggt cgagcgcccc
     1801 cggaggcagc ggcgaagcga gcgtggactg cgatgcggt
//
LOCUS       DBNGSH010000507         1774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3783552, whole genome shotgun sequence.
ACCESSION   DBNGSH010000507 DBNGSH010000000
VERSION     DBNGSH010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3783552"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..1208)
                     /locus_tag="AC16CY_11665"
     CDS             complement(411..1208)
                     /locus_tag="AC16CY_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354495.1"
                     /translation="MYTNLKRGRAVSMALCGLATALVVAAIAIDRAQAQSTSGQIPRQ
                     ADGKPDFSGMWDNPKEPGAKGPATVFNRSRMSPFVPGGEALFYEPRTGDPRHDEPRAF
                     CMPSGFPSAFLGPYPIQLIQTPQYLVMSTEFMNVTRIIPLDGRPHKTDIEPTFYGEPV
                     GRWEGDTLVIEARNYKRWSLDDWYYQNPKEYRMHTEALRTIERLRRVDLNTISYQFTV
                     DDPKIFTKPWTVDWEMKRHPEWEKTGLYEMVCNENNRCEGGKCRDSK"
     gene            complement(1220..1633)
                     /locus_tag="AC16CY_11670"
     CDS             complement(1220..1633)
                     /locus_tag="AC16CY_11670"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354496.1"
                     /translation="MQNPKGKMQNAAAVFVFLLATAPCIQAHHSFTAEYDSNQPVKVT
                     GVVTKVEWTNPHIWFFVDVKDAQGRVTNWGFSGGPPGVLQRRGITKNAMKTGDVIVVE
                     GFRARDGSNNASGGRVTFPDGRSVFTASNEDKAPK"
BASE COUNT          302 a          576 c          550 g          346 t
ORIGIN      
        1 gccccgccga tcaggatcga gcggacgaac ttcgagagga cgaagccttc ccacggcgga
       61 tctttgtagc cctgccagct cgacacgatc gtgccggcaa ccaggccgat gagcggatat
      121 gtccacatga tggccaggat cagcttgaca ccgcgacgga cggagcgtat tggtagctca
      181 gagccccgtc actcgaagac gaggaggttc gtatggagga accaccgggc tagttggccg
      241 catcgattgg gcgcccgcgg gcgtcttcat cgatgcgcga gtgacaatcg aaacgttgtg
      301 caatccatcg atcccggctc ctgccgggat gcgtgcccgt gcacgcagag taaaacgccc
      361 gacactgagg tcttcctcag gtcgggcgtt gccgtctcga agccgaggca ctacttcgaa
      421 tcgcggcact tgccgccttc gcatcgattg ttctcgttgc acaccatctc gtagagcccc
      481 gtcttctccc attcggggtg ccgcttcatc tcccagtcga cggtccaggg cttggtgaag
      541 atcttcgggt cgtccaccgt gaactgatac gagatggtgt tcagatccac ccggcgcagg
      601 cgctcgatcg tgcggagcgc ctcggtgtgc atccggtact ctttcgggtt ctggtagtac
      661 cagtcgtcga gcgaccaccg cttgtagttc ctcgcttcga tgaccagcgt gtcgccttcc
      721 cagcgcccga cgggctcccc gtagaaggtc ggctcgatgt ccgtcttgtg aggccggccg
      781 tcgagcggga tgatgcgcgt cacgttcatg aactcggtgg acatcaccag atactggggc
      841 gtctgaatca gctggatcgg atacgggccg aggaacgccg acggaaaccc cgagggcatg
      901 cagaaggcac gcggctcgtc gtgccgcggg tcccccgtcc tcggctcgta gaacagcgcc
      961 tcgccgcccg gaacgaacgg agacatcctg ctccggttga acacggtcgc cggccccttc
     1021 gccccgggct ccttcgggtt gtcccacatg ccggaaaagt cgggcttgcc gtcggcctgg
     1081 cgtggaatct gaccggacgt ggattgtgcc tgcgcgcgat cgatcgcgat ggcggcgaca
     1141 acgagcgccg ttgccaaccc acacaacgcc atcgagacgg ctcggcctcg cttcagattc
     1201 gtgtacatga ctacgtgcct catttcggag ccttgtcttc gttggaggcc gtgaacacgc
     1261 tacggccgtc gggaaacgtc acgcggccgc ccgaggcgtt gttcgacccg tcgcgggcgc
     1321 ggaacccctc gacgacgatc acgtcgccgg tcttcatcgc gttcttggta atgccgcggc
     1381 gctgcaggac gccaggaggt ccgccggaga atccccagtt cgtgacgcgc ccctgcgcgt
     1441 ccttcacgtc aacgaagaac cagatgtgcg ggttcgtcca ctcgaccttc gtgaccacgc
     1501 cggtcacctt gaccggctga ttcgagtcgt attccgccgt gaacgaatga tgtgcctgga
     1561 tgcacggcgc cgtggcgagc aggaacacga acacggcagc ggcgttttgc atcttgcctt
     1621 ttggattttg catttcgatc gtccggttca gttcgccggc gccatcatga cctggccggt
     1681 cttctggtta cagccgcccg ccgggaacgt ctcgccacac ttgatgagcg caccgccaat
     1741 ggccccgaac ggcttgccat cgcgcagcgg gtgc
//
LOCUS       DBNGSH010000508         1983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1406466, whole genome shotgun sequence.
ACCESSION   DBNGSH010000508 DBNGSH010000000
VERSION     DBNGSH010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1406466"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="AC16CY_11675"
     CDS             <1..807
                     /locus_tag="AC16CY_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3354497.1"
                     /translation="SSEAAIAQRIVRAKKAIAKAGLTFEVPRGAEREARLPSVLEVIY
                     LIFNEGYAATAGDDLIRPGLCLEAQRLGRILAGLMPEDAEVLGLLALMEIQASRLTAR
                     TGPDGASIPIIEQDRARWDYLLIRRGLDALARAEALGGDGPYVLQAALAACHARARRA
                     GDTDWPRIAALYDRLRVVAPSPVVDLNRAIAHSMAFGPDAGLKLVDEIADAAALRDYA
                     PLPAARGDFLFRAGRLAEARSEFTAAATLTRNARERAFLLARADACDCKH"
     gene            966..1913
                     /locus_tag="AC16CY_11680"
     CDS             966..1913
                     /locus_tag="AC16CY_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006436356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3354498.1"
                     /translation="MKLSLFAALAAGLAIPAFGLSRRYRRYLNAARARLAAVDRHVVS
                     TPWGVVEYAERGSGDPVLVVHGIFQNCLSGLLSVRDFSDRRFIAPSRFGYLGSSMPLD
                     ATPAHQADAFAALLDALDIDQIDVIGESAGATSALQLALRHPERVKHLALLVGNLPGS
                     PTAIVQPSWTKRVNRQFVMWALRTVAPSTTARLVAAVPRGFAMSSDDVRYVNEFIDSL
                     FPMSPEGYNFDLFVSNADVTNYDLEAIRVPTLIAHTKDDQLASHEASKRAAERIPGAR
                     FVSFESGGHLMLGQMKIVGDQLDEFFAERRDRRADRVAS"
BASE COUNT          319 a          679 c          635 g          350 t
ORIGIN      
        1 tcgagcgagg ccgcgatcgc gcagcggatc gtccgcgcga agaaggcgat cgcaaaggca
       61 ggattgacct tcgaggtgcc gcggggcgcc gaacgggagg cgcggctgcc ctcggtgctc
      121 gaggtcatct acctcatttt caatgaaggc tatgcggcca cggcgggcga cgatctgatc
      181 cggccgggcc tgtgtctgga ggcgcaacgg ctcggccgca tattggccgg gctgatgccc
      241 gaggatgccg aagtgctcgg cctcctggcc ctgatggaga tccaggcgtc gcgcctcacg
      301 gcgcgcaccg ggccggacgg cgcctccata ccgatcatcg agcaggatcg cgcgcgctgg
      361 gactatttgc tgatccgccg gggcctcgat gcgctggccc gcgccgaagc gctgggcggt
      421 gacggcccct acgtgctgca agccgcgctc gccgcctgcc acgcccgcgc gcgccgggcc
      481 ggcgataccg actggccgcg cattgccgcg ctctatgacc ggctgcgcgt cgtggcgccc
      541 tcgcccgtgg tcgatctcaa tcgcgcgatc gcccacagca tggccttcgg gcccgacgcg
      601 ggcctcaagc tcgtggacga gatcgccgac gccgccgccc tgcgcgacta cgcgccattg
      661 ccggcggcga gaggcgactt tctgttccgg gccggccggc tggccgaggc gcgttcagaa
      721 ttcacggctg cggccacgct gacccgcaac gcgcgggaaa gggcctttct cctcgcacgg
      781 gcggacgcct gcgactgcaa gcactgaatc cggtttccga gggcgccgcc gcgaaaggag
      841 acggcacgcg ccgaaattcc accagcaccc gtggaatttt ccatcgcacc aacgcaacga
      901 aacattgacg cagccgcgcg tttgctgggc agcggccggc acaatcgttg cccttcagca
      961 ggctcatgaa gctgtcgctc ttcgcggcac tcgctgccgg cctggcgatc cccgcattcg
     1021 gcctctcccg gcgttaccgc cgttatctga acgctgcgcg cgcgcgcctg gccgcagtgg
     1081 accgccacgt cgtctcgacg ccatggggtg tcgtcgagta cgcggaacgg ggctccggcg
     1141 atccggtgct ggtcgtgcat gggatcttcc agaactgcct cagcggactc ctttccgtcc
     1201 gcgacttttc cgaccgtcga ttcatcgctc cgtcgcgatt cggttatctc ggctcgagca
     1261 tgccgctcga cgcgacaccg gcacatcaag ccgacgcgtt cgccgctctg ctcgacgcgc
     1321 tcgatatcga tcagatcgac gtcatcggcg aatcagccgg tgcgacatcg gcgctgcaac
     1381 tggccctgcg gcatcccgag agggtgaagc atcttgctct gctcgtgggg aacctgccag
     1441 gcagtccaac cgccatcgtt caaccgtcct ggacaaagcg cgtcaacagg caattcgtga
     1501 tgtgggcact caggacagtg gccccgtcaa cgacggcccg cctggtggct gcggttccga
     1561 gagggttcgc gatgtccagc gatgatgtcc ggtacgtcaa cgagttcatc gacagtctgt
     1621 tccccatgag ccccgagggt tacaacttcg acctgttcgt atccaacgca gacgtcacca
     1681 attacgacct cgaggccatt cgcgtgccca ccctgatcgc ccacaccaag gatgaccagc
     1741 ttgcctctca cgaggcgagt aagcgcgcgg ccgagcgcat ccccggcgcg cggtttgtca
     1801 gcttcgaatc gggaggtcac ctcatgcttg gtcaaatgaa gatcgtcggc gaccaactcg
     1861 acgagttctt tgccgaacgg agggatcgcc gcgcggaccg ggtggcatct tgagcgcagg
     1921 cggaaggagg atctcgtgct tctcacactc tttgtgattc tcacgttctc cgtgctgtat
     1981 tgg
//
LOCUS       DBNGSH010000509         2973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2935371, whole genome shotgun sequence.
ACCESSION   DBNGSH010000509 DBNGSH010000000
VERSION     DBNGSH010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2935371"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2432)
                     /locus_tag="AC16CY_11685"
     CDS             complement(<1..2432)
                     /locus_tag="AC16CY_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3354499.1"
                     /translation="MFERYTERARRVLFFARYEASQLGSVSIETEHLLLGLIREGKGL
                     TSRIFARSHLSLENIRKEIEGRTVFREKVSTSVEIPFSVETKRVLSCAAEEADRLLHN
                     YIGTEHLLLGILREERSVAASILMEKGMRLHTVREDIVQLLNEKTTTTRAKETPLLAE
                     FSRDLTEAAMKNQLDPLVGRDYELERVQQVLCRRTKNNAVLIGEPGVGKTAIVEGLAQ
                     KVVYGDVPHFLADKRLLALDISLIVAGTKYRGQFEERLKAIMKELTENPNIIVFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIRCIGATTPSEYRKYIEKDRSLERRFQAIKV
                     DPPAEKETIEILKGVKDRYEQFHRVEYTPDAIEAAVYQSSRYITDRFLPDKAIDLVDE
                     AGARAKLKEAGYSDEFGEINKSIRVAVEQMENAVSQKDFEKAQFYRDQEASARENLQF
                     ARERFDVKSSGRRVVVSKSDIDEVVSKWTGVPISSINQDEGDRLLRMESDLHRRVISQ
                     DKAISAISRAIRRSRAGLKNPNRPVGSFIFLGPTGVGKTELARALANVLFGSDGALIR
                     FDMSEYMEKHSVSKLIGSPPGYVGHEEGGQLTEKVKRNPYSVVLLDEIEKAHPDIFNI
                     LLQVFEDGHLTDGLGNRVNFKNTIVIMTSNIGARFIQKKASLGFQASDTGTIDKNVTD
                     MVLGEVKRTFNPEFINRIDEIIVFEALSDDDLRRIMRLLLDQLNANLSDRRMKVVLAR
                     EVVDWIIDVTCKDRSYGARPLRRAIQRYVEDPLSEELIRGNLRDGEIEVYLEGGQISY
                     RPAGELVAGRRL"
     gene            2704..>2973
                     /locus_tag="AC16CY_11690"
     CDS             2704..>2973
                     /locus_tag="AC16CY_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3354500.1"
                     /translation="MPDTIGFIGLGLMGRPMAKNLLKAGHPLVVHSRSEGPVAELVAA
                     GAERAASPADVARRAARIITMVPDSPDVELVLEGPNGVFSAIQKGT"
BASE COUNT          474 a          962 c          931 g          606 t
ORIGIN      
        1 ttgagccttc gcccggccac cagttccccg gccggacgat aggagatctg gccgccttcc
       61 aggtagacct cgatctcgcc gtcccggagg ttgccgcgga tgagttcctc ggagagcggg
      121 tcttccacgt agcgctgaat ggcccgccgg agcggacgcg cgccgtacga ccggtccttg
      181 cacgtgacgt cgatgatcca gtccaccact tcccgcgcga ggacgacctt catgcgccgg
      241 tcggacaggt tcgcgttgag ctgatcgagc agcagcctca tgatccgccg caggtcatcg
      301 tcggagagcg cctcgaagac gatgatttcg tcgatgcggt tgatgaactc ggggttgaac
      361 gtccgcttga cctccccgag caccatgtcg gtgacgttct tgtcgatcgt ccccgtgtcg
      421 cttgcctgga acccgagcga cgccttcttc tggatgaagc gcgccccgat gttcgaggtc
      481 atgatgacga tcgtgttctt gaagttcacc cggttgccga gaccgtcggt caggtggccg
      541 tcttcgaaca cctgcagcag gatgttgaag atgtccgggt gcgctttctc gatctcgtcg
      601 agcagcacga cggagtacgg gttccgcttc accttctccg tgagctgacc gccctcctca
      661 tggccgacgt agccgggggg cgagccgatc agcttcgaca ccgagtgctt ctccatgtac
      721 tccgacatgt cgaagcgaat cagggcaccg tcgctgccga agagcacgtt ggccagcgcc
      781 cgggcgagtt ccgtctttcc gacaccggtg gggccaagga agatgaagct gcccacgggg
      841 cggttcgggt tcttgagtcc ggcacgggac cggcgaatgg cgcgtgagat cgccgagatc
      901 gccttgtcct ggctgatgac ccggcggtgc aggtccgact ccatccgaag gagcctgtcg
      961 ccttcgtcct ggttgatcga cgaaatcggc acgccggtcc acttcgagac gacttcgtcg
     1021 atgtccgact tgctgacgac cacgcggcgg ccgctcgact tcacgtcgaa gcgctcgcgc
     1081 gcgaattgca ggttctcgcg cgccgacgct tcctggtcgc gatagaactg cgccttctcg
     1141 aagtccttct gcgagacggc gttctccatc tgctcgacgg cgacgcggat gctcttgttg
     1201 atctcgccga actcgtcgct gtagcccgcc tccttgagct tggcgcgggc tcccgcctcg
     1261 tcgaccagat cgattgcctt gtcaggcagg aaccggtcgg tgatgtagcg gctcgactga
     1321 tagacggccg cctcgatcgc gtcaggggtg tactcgacgc gatggaactg ctcgtagcgg
     1381 tccttgaccc ccttgaggat ctcgatcgtc tctttctcgg cgggcgggtc caccttgatc
     1441 gcctggaacc tgcgctcgag cgaccggtcc ttctcgatgt actttcggta ctcggacggc
     1501 gtcgtcgcgc cgatgcagcg gatctcgccc cgggacagcg ccggcttgag gatgttggcc
     1561 gcgtcgagcg atccttcggc cgacccggcg cccaccagcg tgtggagctc gtcgatgaac
     1621 acgatgatgt tcgggttctc ggtgagctcc ttcatgatcg ccttgaggcg ttcctcgaac
     1681 tgcccgcggt acttggtccc ggcgacgatg agagagatgt cgagcgccag cagccgcttg
     1741 tcggcgagga agtgaggcac gtccccgtac acgaccttct gcgccaggcc ttcgacgatg
     1801 gccgtttttc cgacacccgg ctcgccgatg agcacggcgt tgttcttggt ccgccggcac
     1861 agcacctgct gcactcgctc gagctcgtag tcccgtccga ccagcgggtc cagctggttc
     1921 ttcatcgccg cttcggtcag gtcgcggctg aactcggcca gcagcggcgt ttccttcgcg
     1981 cgggtcgtcg tcgtcttctc gttgaggagc tggacgatat cctcccggac ggtgtggagc
     2041 cgcatcccct tctccatgag aatggaggcg gcgacgcttc gctcttcgcg caggatgccc
     2101 aggagcaggt gttcggtccc gatgtagttg tgcagcagcc gatcggcctc ctcggcggcg
     2161 cacgacagca cgcgcttggt ctcgacgctg aaggggatct cgaccgacgt ggacactttc
     2221 tcgcggaaaa ccgtgcggcc ttcgatttcc ttgcggatgt tttcgagcga cagatgcgag
     2281 cgcgcgaaga tccggctggt cagtcccttg ccttcccgga tcaggcccag cagcagatgc
     2341 tcggtctcga tggacacgct accgagctgg ctggcctcat atcgggcaaa aaagagaact
     2401 cgtcgcgccc gttctgtgta ccgttcgaac atccggcctg ccctggggtt atgcggttgc
     2461 cagcaaagag cttgttgacg ccattataag gcaaagcgct gagccgacca acgctgtcac
     2521 ccgcctaagt cctacgtcct ctatggtttc gacgtgcgct tcggtcagat gcctgaggtg
     2581 ctgactgcca cacacgccag cttatcggca ggtgcacgct tgcacctcac tgcccagcac
     2641 ccaacattct ttcgatgaga cgccacagaa gttagtagtt gaccggccca atatactcgg
     2701 cgcatgccag acacgattgg attcattggc ctcggcttga tgggacggcc gatggcgaaa
     2761 aacctcttga aggccggcca tccgctcgtc gtccacagcc gcagcgaggg accggtcgcc
     2821 gagctcgtcg ccgccggggc ggagcgtgcc gcgtctcctg ccgacgtcgc ccggcgcgcg
     2881 gcacgcatca tcacgatggt ccccgactcg cccgacgtgg agctggtgct cgagggtccg
     2941 aacggcgtgt tcagcgcgat ccagaagggc acg
//
LOCUS       DBNGSH010000510         9791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2765717, whole genome shotgun sequence.
ACCESSION   DBNGSH010000510 DBNGSH010000000
VERSION     DBNGSH010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2765717"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="AC16CY_11695"
     CDS             <1..1350
                     /locus_tag="AC16CY_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3354501.1"
                     /translation="LLAEDALAAVDPLAPKTPHVTPRAKSVIWCFMEGGPSQVDLFDP
                     KPALERYAGQPIPPSYHPETLITAFGTSKNGLFPTRRVFTQRGQSGLWVSDWIPHIAE
                     HADDLAVIRSCQSDAVNHVGGVCQMNTGNILAGRPSMGAWVTYGLGSAQRNLPTFVVM
                     QDDKEILGGVQNYGSGFLPATFQGSLFRQGDTPILNLKPQAGTTDAQQRSKIELLRSL
                     NERFQRQREDDSDLDARTRSYELAYQMQSAAPEAIDLSSETDATRRLYGMDQTETQVF
                     GANCLMARRLVERGVRFVEVYSGSGSGWDAHSDLASNHDKWCRASDKPVAGLLADLKS
                     RGMLKDTLVVWGGEFGRTPFAQIGAGAPPEKYGRDHNPWGFTIFMAGGGVKGGQTIGT
                     TDEIGLRAEEEPYHVNDIHASILYLLGLDHLKTTYLHNGRQERPTGTGGKLITKLWT"
     gene            1422..1898
                     /locus_tag="AC16CY_11700"
     CDS             1422..1898
                     /locus_tag="AC16CY_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3354502.1"
                     /translation="MSPWSVVVGAFALLSMASPGVTAPVEVQAAQFPENPGREIFEII
                     CSSCHQPTVVLDKRWTKAAWQAKVTEMLQEEIDVTETERTQIVGYLAKSFPDRVNVNT
                     ATAAELERIAQIPGDSASAIVAHRASNGMFKTVEDVKQVPGVDAARVEERKARLEF"
     gene            1939..3672
                     /locus_tag="AC16CY_11705"
     CDS             1939..3672
                     /locus_tag="AC16CY_11705"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HIR3354503.1"
                     /translation="MRTLTRTSLIVALTLVAVVAAPGPRTQALQRAADVPLLSILRSE
                     LRRNFDALNKGGAPAYFIGYTVHDEHSGQIVASFGALERSDESQGRFGSVEVRVGKYS
                     LDNTHPIRGEAGGGTPRVGRISLPLADQDKPIALAFWRTTDRAFKVATEALTRVTANV
                     AAKAIQANPAPDFSLEAPHTSIGTPVTYTLDTRQWEARLRRVSAVFADDPLIFDGSAS
                     LGVRATTRYYVNSEGSQILTGDLQYRVFLQAMTKASDGMELPLYNSYFSRSADGLPDE
                     KTLLADARDMKDLLGRLRRAPLVDPYSGPAILSGRAAGVFFHEIFGHRVEGHRHRNSD
                     DGQTFGGKINEAVLPSFLSVAFDPTATTRANVELMGHYRFDDEGVEARRVTVVDQGIL
                     KTFLLGRAPLLEFPRSNGHGRGEPGVSPVSRQSNLMVESSRAVTSQALVSMLKAEARK
                     QGKPFGLLFDNIEGGFTTTGRSNPNAFNVMPNVVYRIYTDDRPPELVRGVDLIGTPLA
                     AFGKFIAASDAISVFNGVCGAESGNVPVSASSPELLVSEVEVQKKAQSQEPLPILPAP
                     TRTTRSTLQRK"
     gene            3669..5360
                     /locus_tag="AC16CY_11710"
     CDS             3669..5360
                     /locus_tag="AC16CY_11710"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HIR3354504.1"
                     /translation="MRACQRLFWLAVVAVVLAPSLRPAVAQDTPILAAMADELARSMA
                     SLRLAGQPAPYFIEYEVEDRISTRATARLGALLEDLHARVRTLRVGVRVGDYDFDSSL
                     FGAPGAPFQDATTTAPLDEDYDAMRRQIWLATDAAYRRAISLFARKKAAFQNRAASDV
                     VPDFSREAPVETVLTGLPATRAASDWPERARQVSAAFAPVRGLDSSEVSVADTSGTRY
                     YLNSEGFKVVAPIQIASLRASAETQASDGMTLRDAFTIVERNLQDLPPVPEIASRARQ
                     MAEQLQAQRAAPIGEEYTGPVLLEGQASAEIVMQGLVPAMLARRAPEFAGRGGGRGGR
                     AGGGGDVTPFLRRIGLRVLSEPFTASDTPSLREFEGRPVPGAYAVDDYGIRAKDVTLV
                     EKGRLVTLLAGRAPLRGLLQSSGHTRGGDVQAGVFQLRSADGVSAAELRRKYLQLLAT
                     QGMPFGYIIRRLGSPGDGPGAGPGAGPMIAQAVKVSPDGREHVVRGVRLGAVPPATFR
                     DLLDASREQTLYSARSANTSAVSVIVPNLIFEELEIQRTREILQKPPVVPSPLGD"
     gene            complement(5550..9287)
                     /locus_tag="AC16CY_11715"
     CDS             complement(5550..9287)
                     /locus_tag="AC16CY_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3354505.1"
                     /translation="MATLSHAWRSWKGAKGVALLAILAFAIGIGSATAIFTVINGVLL
                     RPLPYAQSERFVSLYGARFTEPGQYSSSSTADLREYQSATTSFDVFGWFRLGNFNLTS
                     PGEPQYVAGAAVTPSLAQNLGVPPALGQWFNDESGAVISNSLWRRLGGSPDIVGTALT
                     LDERQLTITGVMPPEFRLPIQGTTTGRLQSDVWIYLDPSGANQPEDSFYFAYARRKPG
                     VPLQQAQADAARVAADIARREPATHPSYTATVVDLRRDTLSDLSETLLLLFAAAGLLL
                     LIACANVATLLLARSVTRARETAVRLALGAGRGHLALKYLVEAAAVSLVGAAAGVGLS
                     AALVRVVLVVGSEYLPDADDIAIDWKVVSFGLAMAFLASALASLAPLWHALRTPPNAV
                     LTEGVRATAGASVRRLSQALVVAEIALAFTLLAVSTILVVHLGNLRRVPTGFNPDNVL
                     AFELTAPARIVNSKTLVPFQKRLLEALEAVPGVTGAALVNQLPLDGCCVGGTVYPEGV
                     PPGEDARRVAFLFVTPGYMTAMGIPLRAGRFLTDADTNDKELFAVVNQAAVNRYWPDR
                     NPVGAFGRLNRPDGNRFQVVGVVGDIRNDGLNAAPEAEVYLLATMIPVNPMNIIVRSP
                     LPTGQLLLEVRRALQSVERTLTIRNARAMTDVVSDSLQLERVSSVMMSGFALAALLMA
                     TLGIYGVVSYAVRQRTVEMGTRMALGAVSRDLFALVVGGGFRMAGLGLAVGVPALAAS
                     VWLLAQFMEIRDIGWVPFASSTVVVALIASVASYVPAWRASLLSPMVAIRDESTSAWS
                     ARRRFLSAVRGAGNAVSAPAAAPPTLLTEFVAAARAADSFDEALAKALGTLCRSLGVD
                     AAVLLEAAGGAHRARIAIGPFEPIAWAVPADGFLASRLAAYPLPLPFDPGEFDALREW
                     AAAHRPDRLEEIRLLASADVRMAVPLRTRTEIMGVLLMGASRDRAHFGPVEKQILANC
                     ADQFALMIENARLTDRVVGQEALRRDLALAAEVQRRLLPADAPSADIAEFAAVSLPAR
                     SIGGDYYDFIQVGEQRIGIALADVSGKGITAALIMSVVQASLRIIASDGDISLPRLAA
                     RMNDFLYRTTPGNKYATFFYARVDGDRRQLRYVNAGHNPPYLVRMRRPGADESEADPP
                     MEELTIGGAVVGMLPGMSYEEATVDLCSGDVLLAYTDGVTEAHNPDNVEFGEDRLKAL
                     LRGVVHLSAEEIRARIEQELKDWIKDAEQYDDLTFVVMKVH"
     gene            complement(9358..>9791)
                     /locus_tag="AC16CY_11720"
     CDS             complement(9358..>9791)
                     /locus_tag="AC16CY_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354506.1"
                     /translation="LVDVDVPKFSDAPLSMSGVVLASFLELPQTAVKRDDPLLRLLGS
                     AYPTANRSFARNDRLVVYLEVYTNGKTRLTDTVATIAGVNQRRTRTLDLPRIIVEPQR
                     AAFRQFLPLRAYEAGNYVLSVEATAGREKVSRQVPFTIREQ"
BASE COUNT         1685 a         3300 c         3315 g         1491 t
ORIGIN      
        1 ctgctggccg aggatgcgct cgccgccgtg gacccgctcg cgccgaagac gccgcacgtc
       61 acgccgcgcg ccaagtcggt catctggtgc ttcatggaag gcggaccgag ccaggtggat
      121 ctgttcgacc ccaagcccgc gctcgagagg tacgcgggcc agcccatccc gccgtcctat
      181 caccccgaaa cgctcatcac ggccttcggc acgtcgaaga acggactgtt tccgacacgg
      241 cgcgtgttca cgcagcgagg ccagagcggc ctctgggtct cggactggat tccgcacatc
      301 gccgagcatg ccgacgatct cgcggtcatc cgttcctgcc agtccgacgc cgtcaaccac
      361 gtgggcggcg tgtgccagat gaacaccggc aacatcctgg ccgggcgccc gtcgatgggt
      421 gcgtgggtca cctacggcct cggcagcgcg cagcgcaacc tgccgacctt cgtcgtcatg
      481 caggacgaca aggagatcct cggcggcgtg cagaactacg ggtccgggtt cctgccggca
      541 accttccagg gctcgctctt ccgccagggc gacacgccga tcctgaacct gaaaccgcag
      601 gctggaacga ccgacgcgca gcagcggagc aagatcgagc tgctgcggtc gctcaacgaa
      661 cgattccagc gccagagaga ggacgactcc gatctcgatg cgcgcacgcg gtcctacgag
      721 ctcgcgtatc agatgcagtc ggccgcgccc gaagcgatcg acctgagcag cgagaccgac
      781 gccacgcggc gtctctacgg catggaccag accgagacac aggtgttcgg cgccaactgc
      841 ctgatggccc gccggctcgt cgagcggggc gtgcgcttcg tggaggtcta cagcggctcc
      901 gggtccggct gggacgcgca cagcgacctc gccagcaacc acgacaagtg gtgccgcgcc
      961 tcggacaagc cggtggccgg attgctcgcc gatctgaaat cgcgcgggat gctgaaggac
     1021 acgctcgtcg tgtggggcgg cgagttcggc cgcacgccgt ttgcgcagat cggcgccggc
     1081 gcgccgccgg agaagtacgg ccgcgatcac aacccgtggg gcttcacgat cttcatggcc
     1141 ggcggcggcg tcaagggcgg ccagacgatc gggacgaccg acgagatcgg cctgcgcgct
     1201 gaagaagagc cgtaccacgt caacgacatc cacgcctcga tcctgtacct gctcgggctc
     1261 gaccacctga agacgaccta cctgcacaac ggccggcagg agcgcccgac gggcacgggc
     1321 gggaaactga ttactaaact gtggacctag gcagtcagat ccacgaacca cggaggcacg
     1381 gagccacgga gaacgttttc aagaattgtc tccgtgtctc cgtgtctccg tggtctgtgg
     1441 tcgtgggcgc cttcgcgctg ttgtcgatgg catcgcctgg ggtcacggca ccggtcgagg
     1501 tccaagctgc ccagttccct gagaatcctg gtcgagagat cttcgagatc atctgcagct
     1561 cctgccacca gccgaccgtc gtgctcgaca agcgctggac gaaagcggcg tggcaggcga
     1621 aggtgaccga gatgctgcag gaagagatcg acgtcaccga gacggaacgc acgcagatcg
     1681 tcggctacct cgcgaagagc tttcccgacc gcgtcaacgt caacacggcg acggccgccg
     1741 agctcgagcg catcgcgcag atccctggcg acagcgcctc ggccatcgtc gcgcatcggg
     1801 ccagcaacgg gatgttcaag acggtcgagg acgtgaagca ggttccaggc gtggacgcgg
     1861 cgcgcgtcga ggagcgcaaa gcgcgcctgg agttctgagc cgcggccgcg aagtcacgaa
     1921 gaggatggcc atacgcgcat gcggacactg acacggacgt cgctcatcgt cgccctgacc
     1981 ctcgtcgccg tcgtggctgc gcccggccct cgcacgcagg cgcttcagcg ggcggcggac
     2041 gtgcctctct tgagcatcct gcgatcggag ctgcgacgga atttcgacgc gctcaacaag
     2101 ggcggcgccc ccgcctactt catcggctac acggtccacg acgagcactc cgggcagatc
     2161 gtggcgtcgt tcggcgccct ggagcgaagc gacgaaagtc agggacggtt cggcagcgtc
     2221 gaagttcgag tcggcaaata ctcgctcgac aacacgcacc cgatccgcgg cgaggccggc
     2281 ggggggacgc cgcgcgttgg ccgtatctcg cttccgctgg ctgatcaaga caaaccgatt
     2341 gcgctcgcct tctggcgcac aacggaccgc gcgttcaaag tcgccacgga agcgctcacg
     2401 agggtcacgg ccaacgtcgc cgcgaaggcg atccaggcga acccggcgcc ggatttctcg
     2461 ctggaggcgc ctcacacgtc catcggaaca ccagtgacct acaccctgga tacccggcag
     2521 tgggaagcgc gccttcgcag ggtgtcggcg gtgttcgccg atgacccgct catcttcgac
     2581 ggctcggcgt cgcttggcgt ccgggccacc acccgctatt acgtgaacag cgaaggctcg
     2641 cagatcctca cgggcgatct tcagtaccgc gtgttcctcc aggcgatgac gaaggccagc
     2701 gacgggatgg agctgccgct ctacaacagc tacttctccc ggtcggcgga tgggctgcca
     2761 gacgagaaga cgctgctcgc cgacgcacgg gacatgaagg atcttctggg gcggctgcgc
     2821 cgcgcgccgc tcgtcgaccc gtactcggga cccgcgatcc tgtccggccg cgccgccggc
     2881 gtgttcttcc acgagatctt cggccatcgc gtcgaagggc accgtcaccg gaactcggac
     2941 gatggccaga ccttcggcgg caagatcaac gaggccgtgc tgccgtcgtt tctgagcgtg
     3001 gccttcgatc ccacggcaac cacgcgcgca aacgtggagc tgatgggcca ttaccgcttc
     3061 gacgacgagg gcgtcgaggc acgacgggtg acggtcgtcg accaggggat cctcaagacg
     3121 ttcctgctgg ggcgtgcgcc gctcctcgag ttcccgcggt cgaacggtca cgggcgcggt
     3181 gagccgggcg tctcccccgt gtcccgtcag tcgaacctga tggtggagtc gagtcgtgcc
     3241 gtcacctcgc aggcgcttgt ctcgatgctg aaggcggaag cgcgcaaaca ggggaagccg
     3301 ttcggcctgc tcttcgacaa catcgagggc ggcttcacga cgaccggccg cagcaatccg
     3361 aacgccttca atgtcatgcc gaacgtcgtc taccgcatct acaccgacga ccggccgccg
     3421 gagctcgtgc gcggcgtgga tctgatcggc actccgctgg ccgcgtttgg caagttcatc
     3481 gcggcaagcg acgcgatcag cgtgttcaac ggcgtgtgtg gcgccgaaag cggcaacgtg
     3541 ccggtgtcgg cgagctcgcc ggaactgctc gtcagcgaag tcgaagtgca gaagaaggcc
     3601 cagtcgcagg agccgctgcc catcctgccg gcgcccacgc ggacaacgcg cagcaccctc
     3661 cagcggaaat gagagcctgt cagcgcctgt tctggctcgc tgtcgtggcg gtcgttctgg
     3721 cgccgtctct gcggccggcg gtcgcgcagg atacgccgat cctggcggcc atggcggacg
     3781 agctcgcgcg gtcgatggcg tcgctccggc tcgcgggcca gcccgcgccg tacttcatcg
     3841 agtacgaagt cgaggatcgg atctcgacgc gggcgacggc gcggctgggt gcgctcctcg
     3901 aggatttgca cgcgcgagtg cggacgctcc gtgtcggcgt gcgtgtgggc gactacgact
     3961 tcgacagctc gttgttcggt gcgccgggcg ctcctttcca ggatgcgacg acgaccgcgc
     4021 cgctcgacga ggactacgat gccatgcgcc ggcagatctg gctggccacc gatgccgcct
     4081 acaggcgggc catcagcctg ttcgcccgga agaaggcggc cttccagaac agagcggcga
     4141 gcgacgtcgt gccggacttc tcccgggagg ctccggtcga aactgtcctg accgggctgc
     4201 ctgccacgcg tgcggcgtcc gactggccgg agcgggccag gcaggtgtcc gccgctttcg
     4261 caccggtccg aggcctcgac agctcggagg tctccgtcgc cgacacgagc ggcacgcgct
     4321 actacctgaa cagcgaaggc ttcaaggtcg tcgcgccgat ccagattgcg tcgctacgcg
     4381 cgtcggccga aacgcaggcg agcgacggca tgacgctgcg cgacgcgttc accatcgtcg
     4441 aacggaatct gcaggacctg ccgccagtgc ccgagattgc gtcgcgcgcg cggcaaatgg
     4501 ccgagcagct gcaggcgcag cgcgcggcac cgattggcga ggagtacacg ggtccggtgc
     4561 tgctcgaagg acaggccagc gcggaaatcg tgatgcaggg gctcgtgccc gccatgctgg
     4621 cgcggcgggc gcccgagttt gcgggccgcg ggggtgggcg tggaggtcgc gcgggcggcg
     4681 gaggcgacgt cacaccattc ctcaggcgca ttggcttgcg cgtgctgagc gagcccttca
     4741 cggcgtccga cacaccatcg ctgcgtgagt tcgaaggccg gcccgtaccc ggggcgtacg
     4801 ccgtggacga ctacggcatc cgcgcgaagg acgtgacgct ggtcgagaaa ggccggctgg
     4861 tcacgctgct ggccggccgg gctcccctgc gcgggcttct gcagtcgagc ggccacacac
     4921 gcggcggcga cgtgcaagcg ggcgtgttcc agttgcggag cgcagacggt gtgtccgcag
     4981 cggagctgcg gcggaagtat cttcagttgc tcgcgactca gggcatgccc ttcggataca
     5041 tcatccgccg gctgggcagc cccggagacg gacccggcgc cggtccgggc gccgggccga
     5101 tgatcgcgca ggcggtgaag gtgagccccg atgggcgcga gcacgttgtt cgcggcgttc
     5161 gcctcggcgc cgtgcctccg gccacgttca gggatctgct cgacgcgtcg agggaacaga
     5221 cgctctacag cgcacgatcg gccaacacga gcgccgtctc cgtcatcgtc ccgaatctga
     5281 ttttcgagga gctcgaaatt cagcggacga gggagatcct ccagaagcca cctgtcgtcc
     5341 cctccccgct cggcgactga ccgggtccgg ctgaacgcta cgtctgatgc ttcgtgtcac
     5401 cgagcggtcg tagtgtccgg cttcagccgg acccgggaac cgtcaacctt gaacctcgaa
     5461 cacgaacccc gaaactgaac acgaaccgcg aagcgagaag agagaagtgt gaacggctga
     5521 tctcgtgctc aattcatgtg acctctgggt caatgcacct tcatcactac gaaagtgagg
     5581 tcgtcgtact gctcggcgtc cttgatccag tccttcagct cctgctcgat gcgcgcgcgg
     5641 atctcctccg ccgacaagtg gacgacgccg cgcagcagcg ctttgagccg gtcctccccg
     5701 aactcgacgt tgtccgggtt gtgcgcctcg gtgacgccgt cggtgtaggc cagcaggacg
     5761 tcgcccgaac acagatcgac cgtcgcctcc tcgtagctca ttcctggcag catgccgacg
     5821 actgcgcctc caatcgtcag ctcctccatc ggagggtcgg cttcggactc gtcggcgccg
     5881 gggcggcgca ttcgaacgag gtagggcgga ttgtgcccgg cgttcacgta tcgcaactgg
     5941 cgccgatcgc cgtccactcg ggcgtagaag aacgtcgcgt acttgttccc gggcgtcgtc
     6001 cggtacagga aatcgttcat gcgcgcggcc agccgcggga gcgagatgtc gccgtcggac
     6061 gcgatgatcc gcagcgacgc ctggaccacg gacatgatca gggcggccgt aattcccttc
     6121 cccgagacgt cggcgagcgc gatcccgatg cgctgttccc cgacctggat gaagtcgtag
     6181 tagtcgcccc cgatgctccg cgccggcagg ctgacggcgg cgaactcggc gatgtcggca
     6241 gagggcgcgt cggcgggcag gagccgcctc tggacttcgg cggcgagggc gagatcgcgc
     6301 cgcagcgctt cctgcccgac gaccctgtcc gtcaggcgcg cgttttcgat catcagcgcg
     6361 aactgatccg cgcagttggc gaggatctgc ttctccaccg ggccaaagtg cgcgcgatcg
     6421 cgtgaggcgc ccatcagcag cacacccatg atctcggtcc gcgtacgcag cggcaccgcc
     6481 atgcggacgt cggcggacgc caggagacgg atttcttcca gcctatcggg cctgtgcgcc
     6541 gccgcccatt cccgaagtgc gtcgaactcg ccagggtcga agggcagcgg cagcgggtat
     6601 gccgcgaggc ggctggcgag aaacccgtcg gcaggcactg cccaggcgat cggctcgaag
     6661 ggcccgatgg caatccgagc ccggtgtgcg ccgccggcgg cctcgagcag tacggccgca
     6721 tcgacgccca ggctccggca caacgtccca agcgccttgg ccagcgcctc gtcgaacgag
     6781 tccgcggcgc gcgcggcggc cacgaactcg gtcaacaacg tcggaggagc ggccgctgga
     6841 gctgaaacgg cgttgccggc tcctcttacc gcgctcagga agcgtcggcg agccgaccac
     6901 gccgaagtag actcgtcgcg aattgccacc atcggcgaca gaaggctcgc gcgccaggca
     6961 ggtacgtagg acgctaccga cgcgatcagc gcgaccacga ccgtagacga ggcaaacggc
     7021 acccatccga tgtcgcgaat ctccatgaac tgggcaagga gccagacgga cgccgcgagc
     7081 gcgggcactc cgacggcaag tccgagcccc gccatcctga acccgccgcc gacgacgagc
     7141 gcgaagagat cgcggctcac ggcgccaagc gccatccgcg tgcccatctc gactgtccgt
     7201 tgacgaacgg cgtacgacac cactccgtag atgccgagcg tggccatcag cagcgccgcc
     7261 agcgcgaagc cgctcatcat cacggagctg acccgctcga gctgcagcga gtcgctcacg
     7321 acgtcggtca tcgcccgcgc attgcgaatc gtcagcgtcc tttccacact ttgaagggcg
     7381 cgcctcacct ccaacaagag ctgccctgtc ggcagcgggg accgcacgat gatgttcatc
     7441 gggtttaccg ggatcatcgt ggccagcagg tacacttcgg cttcgggcgc ggcgttcagg
     7501 ccgtcgttgc gaatgtcgcc gacgacgccc acgacctgga agcgattgcc gtccggcctg
     7561 ttcagccgtc caaacgcgcc cacgggattc cgatcgggcc agtaacggtt cacggcagcc
     7621 tggttcacca ccgcgaagag ttccttgtcg ttggtgtcgg cgtcggtgag aaaccggcca
     7681 gcccgcagcg gaatgcccat cgcggtcatg tacccgggcg tgacgaagag gaaggcgacc
     7741 ctgcgtgcgt cctcgcccgg agggacgccc tccggataca cggtcccgcc cacgcagcag
     7801 ccgtcgagcg gcagctggtt gacgagcgcc gcgcccgtca cgccgggaac cgcctccagc
     7861 gcttcgagca gccgcttctg aaacggcacg agcgtcttcg agttgacgat gcgcgccggt
     7921 gccgtgagct cgaaggcgag cacgttgtcg ggattgaacc cggtcgggac tcgacggagg
     7981 ttcccgagat gaacgaccag gatcgtcgag acggcgagca gcgtgaacgc gagcgcaatt
     8041 tcggcgacca cgagcgcctg cgacagccgc cgcacggacg cgccggccgt ggcgcggacg
     8101 ccttcggtca gcaccgcgtt gggcggcgtt cgcagtgcgt gccagagcgg ggcgaggctg
     8161 gccagcgcgc tcgccaggaa cgccatcgcg agtccgaacg agacgacctt ccagtcgatg
     8221 gcgatgtcgt cggcgtcggg caggtactcg gacccgacca cgaggacgac ccgcacgagc
     8281 gccgcgctca gcccgacgcc cgcggccgct ccgacgagcg agacggcagc tgcctcgacg
     8341 aggtacttga gcgcgaggtg cccgcggccc gctccaagcg cgaggcggac cgccgtctcc
     8401 cgggcacggg tcaccgatct cgccagcagc agggttgcga cgttggcgca cgcaatcagg
     8461 agcagcaggc cggctgccgc aaacagcagg agcagcgtct ctgaaagatc gctgagcgtg
     8521 tcccggcgca gatcgacaac ggtcgcggtg taggacggat gagtggccgg ctcgcggcgc
     8581 gcgatgtcgg cggccacgcg tgcggcgtcg gcctgggcct gctgcagcgg gacgcccggc
     8641 ttgcggcggg cgtacgcgaa gtagaagctg tcctccggct ggttcgcacc actcgggtcg
     8701 aggtagatcc agacgtcgga ctgcagacgt ccggtcgtgg tcccctggat cggcaggcgg
     8761 aattccgggg gcatcacacc cgtgatcgtg agctgacgtt cgtcgagggt gagcgccgtt
     8821 ccgacgatgt ccggactgcc gccaagccgg cgccagagcg agttcgagat gacggcgccg
     8881 ctctcgtcgt tgaaccactg gcccagcgcg ggagggacgc ccaggttctg cgccagcgac
     8941 ggcgtgacgg ccgctccggc cacgtattgc ggctcgcccg gcgatgtcag gttgaagttg
     9001 ccgagccgga accagccgaa gacgtcgaaa ctggtggtcg cgctctggta ctcacggaga
     9061 tcggccgtcg aactggagct gtactgacca ggctcggtga atctggctcc gtacagcgac
     9121 acgaaccgct cgctctgggc gtagggcagc ggcctcagca gcacgccgtt gatgacggta
     9181 aagatggcgg tggccgagcc gatgccgatc gcaaacgcca ggatcgcgag cagcgcgaca
     9241 cctttcgcgc ccttccacga ccgccacgcg tgcgacaggg tcgccacgcc gcgcacgata
     9301 acacgcgcag gcgcgtacgt agtgtccggc ttcagccgga cccgcgaagg ctccggttca
     9361 ctgttccctg atcgtgaacg gtacctggcg gctgaccttc tctcgtccgg cagtcgcctc
     9421 gacgctcagg acgtagttgc cagcctcgta agcacggagc ggcaggaact gccgaaaggc
     9481 ggcgcgttgg ggttcaacga tgatccgcgg cagatccagt gtccgtgtcc ggcgttgatt
     9541 cacgccggca atcgtcgcga cagtgtcggt caatcgcgtt ttgccgttcg tgtacacctc
     9601 gaggtagacg accagccggt cgtttcgcgc gaacgaccga ttggctgtcg gatatgcgct
     9661 gccaagaagc cgcaacagcg ggtcgtcccg cttcaccgcc gtctgcggca gctcgagaaa
     9721 cgaggcgagc acgacgccgc tcatcgacag cggcgcatcc gagaatttcg gcacgtcgac
     9781 atcaacaagg a
//
LOCUS       DBNGSH010000511         2239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3275061, whole genome shotgun sequence.
ACCESSION   DBNGSH010000511 DBNGSH010000000
VERSION     DBNGSH010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3275061"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="AC16CY_11725"
     CDS             complement(<1..367)
                     /locus_tag="AC16CY_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3354507.1"
                     /translation="MLVRTFVRTTAMLAVALVLSHQAAAQDGVPLAGRLISSLNGSPL
                     AGATVQIDELKRQAVSAADGTFSFDGVPPGTYHLSVFAQGYSTRRTEVRAAAGAPSID
                     LQVDFDLHFEEVVSVSGDAR"
     gene            complement(419..784)
                     /locus_tag="AC16CY_11730"
     CDS             complement(419..784)
                     /locus_tag="AC16CY_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354508.1"
                     /translation="MLAWFRSAARPISSAALASILTLTVWSATPHPDDCHETECLSAP
                     HDPTGHSLGRKQGGGEQPFHCVFCHWTRSVRPPSPTTYQLAPSVEDRARPFVVVSRVL
                     PQFSAAQPPLRAPPASSVA"
     gene            880..>2239
                     /locus_tag="AC16CY_11735"
     CDS             880..>2239
                     /locus_tag="AC16CY_11735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR3354509.1"
                     /translation="MRLSRRQFIHTAATATAAAWPLTVRAQQAAAPRQGGLFRHGVAS
                     GDPLTDRVMLWTRVTPPDANAITPIDVRWVIAADERLTQVVSRGSAQAPAERDFTVKV
                     DAGDLRPGRTYFYAFDTRGEQSPIGRTKTLPDEGASRVRIAQVSCSNYPTGYFNVYRC
                     LANRPDLDVVLHLGDYIYEFAQGRYSDPSLKRTVLPAGETTTLQDYRTRYALYRADVD
                     LQDVHRLHPFIVVWDDHELANNAWARGAGNHRPAQGDWKTRQEAAYRAYLEWMPIRES
                     RTSGIRLFRSFRFGRLVDLLMLDTRGLRDEQVDVKNAAVLSDARRTLLGAEQESWLFD
                     ALRGSQRSGSRWRLLGQQVLFAPMTPPGTPVQNTDVWDGYPAARERVFDMLASERVAD
                     VAILTGDIHSSWALDVPRTPWSGYNPGTGAGSRAVEIVTPAVSSPPMFSSAPLRDAAK
                     LLL"
BASE COUNT          390 a          754 c          757 g          338 t
ORIGIN      
        1 tgcgcgcgtc accgctcacc gacacgacct cctcgaagtg caggtcgaag tccacctgca
       61 gatcgatcga cggcgcgcct gcggcggcac gcacctcggt gcgcctggtc gagtacccct
      121 gcgcgaatac cgacaagtgg taggtgccag gaggcacgcc gtcgaacgaa aacgttccgt
      181 cggcagccga cacggcctgc cgcttgagct cgtcgatctg cacggtcgcg cccgcaagcg
      241 ggctcccgtt caacgagctg atgagtcgtc ccgcaagagg cacgccgtcc tgcgcggcag
      301 cctgatgcga caggaccagg gccacagcga gcatggccgt cgtcctgacg aacgtacgta
      361 caagcatgag gtgtctcccg aatcgagtga gccgatggcg gagtagatcc gccgtggact
      421 acgcgaccga agacgcggga ggcgctcgaa gcgggggctg cgctgccgag aactgcggca
      481 gcacacggga gacgacgacg aagggccgcg cgcgatcttc gaccgagggc gcgagctgat
      541 atgtcgtggg cgacggtgga cgcaccgagc gcgtccagtg gcagaagacg cagtggaacg
      601 gctgctcgcc cccgccctgt ttccgtccga ggctgtggcc agtcggatca tggggcgccg
      661 acagacactc ggtctcgtgg caatcgtcag gatgcggcgt cgcggaccag acggtcagcg
      721 tgaggatcga ggcgagcgct gcgctcgata tcggccgggc cgcggagcgg aaccaggcga
      781 gcatcgaaca gccagcgtaa cacgagtgaa atggggtcgg gagtcatttt ccgaaaatgc
      841 acctgggaaa atgactcccg accccttagg atctctcccg tgcgtctttc gcgccgccag
      901 ttcatccata ctgcggccac ggccacggcc gcagcgtggc cgctcaccgt ccgcgcacag
      961 caggctgccg ccccgcgtca gggcggcctc tttcgtcacg gggtggccag cggcgatccg
     1021 ctgaccgacc gcgtgatgct ctggactcgg gtcacgccgc ccgacgcgaa cgcgatcacg
     1081 ccgattgacg tgcggtgggt gatcgcggcc gatgagcgac tgacccaggt ggtctcgcgc
     1141 ggcagcgcgc aggcgccggc ggagcgggac ttcacggtca aggttgacgc cggagacctg
     1201 cgtccggggc gcacgtactt ctacgcgttc gacacgcgcg gcgagcagtc gccgatcggc
     1261 cgcacgaaga cgctgcccga cgaaggcgca agccgcgtcc gcatcgcgca ggtgtcctgc
     1321 tcgaactacc cgaccggcta cttcaacgtc taccgctgcc tcgccaaccg ccccgacctg
     1381 gacgtcgtgc tgcatctcgg cgactacatc tacgagttcg cccaggggcg gtacagcgac
     1441 ccctctctca agagaaccgt gctgccagcg ggtgaaacga cgacgctgca ggattaccgc
     1501 acgcgctacg cgctgtaccg cgccgatgtc gatcttcagg acgtgcaccg cctccacccc
     1561 ttcatcgtcg tctgggacga tcacgaactg gcgaacaacg cgtgggcgag aggcgccggc
     1621 aaccatcgtc ccgcccaggg cgactggaaa acgcgtcagg aggcggccta ccgcgcgtat
     1681 ctcgaatgga tgccgatccg tgaatcccgc acctcgggga tacggctgtt tcgcagcttc
     1741 cgcttcggcc gcctggtcga tctgctgatg ctggacacgc gaggccttcg cgacgagcag
     1801 gtggacgtca agaatgcggc cgtgttgagc gacgcgcgac gaacgctgct tggagccgag
     1861 caggaatcgt ggctcttcga cgccttgcgg gggtcgcagc gatcaggcag tcgctggcgg
     1921 ctgcttggtc aacaggtcct cttcgcgccc atgactcctc ccggaacgcc ggtccagaac
     1981 accgacgtgt gggacggcta tccggcggcc cgggagcgcg tcttcgacat gctcgccagc
     2041 gagcgcgttg cagacgtcgc catcctgacg ggagacatcc acagctcctg ggccctggac
     2101 gtgccgagga caccgtggtc cggttacaac ccgggcacag gcgcgggatc gcgggcggtt
     2161 gaaatcgtca cgccggccgt gagctcgccg ccgatgtttt cgagcgcgcc cctgcgcgac
     2221 gcggcgaagc tcctgctgg
//
LOCUS       DBNGSH010000512         7362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3444620, whole genome shotgun sequence.
ACCESSION   DBNGSH010000512 DBNGSH010000000
VERSION     DBNGSH010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3444620"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="AC16CY_11740"
     CDS             <1..1677
                     /locus_tag="AC16CY_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3354510.1"
                     /translation="ALGNYYWALGRLTETEQTFRSALKVKPHDPSANRAMAALSMTTG
                     RTAEAEQYLRTIADTFGDSASVVALANYYLLAGRSKDAIDKLTPLATGKEKVPGTPQL
                     LARAYALSGDSGKARELVDTILEAERGDLDAQLLKAQLLYQDGKRDAALALVAAAASA
                     NPTSAEAQLMLGKLHVARGDVAPAEEALREVLRLNPRVTAAQVELSRLLLSTGRTADS
                     LRTAEEAARNEPRSPEARLALVRSLLVAQDAARAQREIATLLADHPEVAEVQAMAGVL
                     AVLKNDVAGGRAAFERALSANANSLEALSGLVALDLRAKDTAGAMKRIDSYVASNPRP
                     EAMLLAARTHASAGDTAGAERYLRRAIEADASMLPAYAMLGELYLSQKRLDEARKEFD
                     SLATRQTKPIAALTMSGIILMTQGQNAQARERFEKALAIDPRAPVAANNLAWMQVEAG
                     ENLDVALQLAQAAASASPDTPEITDTLGWVYYKKNLPQQAIPQFERSLSKAPGNPMFH
                     YHLGLAYLQAGDADRGRASLRRALSAGADAGTAAEIKRVLSAPQAASAKK"
     gene            complement(1697..3193)
                     /locus_tag="AC16CY_11745"
     CDS             complement(1697..3193)
                     /locus_tag="AC16CY_11745"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR3354511.1"
                     /translation="MIRKILLITAAGIVVLLLAAAAGVFWLFSGDGMRLALESQATSW
                     LGHPVRIGGARGQIFPRIGIRLSDVRLGEPARVTLSGVQLSTDLRALLDRRIEDARIV
                     ISDSRLEMPIPFGIPSGSGSSGGSRGKSTGASQPAVKFVSIGEIALRNIRVLSRGREV
                     VVSADSTLEGDTLVVRRFSAQSGGTALEVSGKVDLEPRIDAQLKATANKIDVDELLAL
                     AAAFTPAGAPASRKQVSTPARIAARIESKTAKVGDLEVTQFSTGMEVDGNRIALTPIA
                     FQIFGGRYEGSLNARLGERLTATLQSRVEDVDVAQLAAFGGSPDTVTGRLTANGTFGS
                     QGADMDAIMGSANGKGTASIVDGSIRRLDLVRTVVLFFGKPAAETPAASDRFERFDAS
                     FSLANQVFRADSLSLRSRDADLTGTGTLTLGNEALDGRVNMVLSEELTSQAGTDLVRY
                     TREGNRVVLPARISGTLAKPRVMIDASAAIKRGLINEGSRRLLELFGK"
     gene            3268..4173
                     /locus_tag="AC16CY_11750"
     CDS             3268..4173
                     /locus_tag="AC16CY_11750"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR3354512.1"
                     /translation="MPELPDILLYLHALRSRVVGKRIETVRLVSPFLLRSIDPPLSAV
                     EGRVVTDLHRLGKRIAIETERELFLVFHLMIAGRFRWKPAGTRVPGKVGLLALDFEHG
                     SLILTEAGTKRQASLHVVDGADALARHNPGGLEVMETGAEPFEQALRRENHTLKRALT
                     DPRIFSGIGNAYSDEILHAARLSPFKQTSTLSQEEAHRLYDATRGTLAAWTERLQSET
                     GDGFPEKVTAFREGMAVHGRFGKPCPVCGSAVQRIVYASNEANYCVTCQTGGRLLADR
                     SLSRLLREDWPRTLEDLERRKRGDG"
     gene            4178..5653
                     /locus_tag="AC16CY_11755"
     CDS             4178..5653
                     /locus_tag="AC16CY_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941956.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3354513.1"
                     /translation="MRRPTLTAIVLCLCVGAAAAGQERSELRWGGDAEGGAPFVEADP
                     NDLTRVVGFEVEIAQLLASGLGRAPRFVQSGFTSLDAAAARGDFDVGLSGIEDSPARR
                     SRLAVTIPYYQFREVLTVREGDAGRFRGLGDLAGRRVATLGATLAYDLLLAAQTKFGV
                     VPVTYEDDVHPYTDLVLGRVDAVLLDEVLARRGIRRNPGLTNQPTDVGVGYYVGITAP
                     ANAPLRDRMNEILLQAMADGRLEAIFRRWSMWNDDQPQLYARLARGGQGEIAQAPSPQ
                     QPSTFQAAVRYLPSLFRAAAITLVLSCLSMSLAVALGLAIASGRVYGPPAVRMLLTGW
                     VELIRGTPLLLQLFVLYFGLAAIVQLPAFLAALIGLGLNYAAYESEIYRGALEAVPAG
                     QLDAARTLGFTERQTLVLVRGPQAFRLALAPMTNDFVALLKDSSLVSVITVVELTKQT
                     SILAANIGSWVIPGILCAAMYLVLSLPLARAARRLEGKWKR"
     gene            5722..6381
                     /locus_tag="AC16CY_11760"
     CDS             5722..6381
                     /locus_tag="AC16CY_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR3354514.1"
                     /translation="MSALTIVRGARTLADDVSFSATRGEVVVLRGPSGAGKTTVLRAI
                     AGLDPIAAGRITVDGVAIGPGRTPRARALRELHRRVGMVFQFHHLFEHLTAFENVWLA
                     PVHVLRQPRAEAERRAHELLEQLGIGARARALPRELSGGEAQRVAIARALAVGPQVLL
                     MDEPTASLDTERRDSLAGTLRQLAAGGCTLVLASHDEEFIRACSHRVILMQAGRVVGP
                     S"
     gene            6397..7128
                     /locus_tag="AC16CY_11765"
     CDS             6397..7128
                     /locus_tag="AC16CY_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354515.1"
                     /translation="MPGPQIPTDSLFVELDTDRKIRHRNKLYYRFNHWPIWVFVFFIA
                     PGPLTFDLFERGFDSRLVVWLGIVLVGTAVAGLLGKLPGCEPRPYIIRFTEDRPNPLY
                     RRICYTTAWGEVVAFAVLNIAGLAYAVATGQWRLRQMYEVAYFPLAGTVWVLGALGQL
                     PRVKASTKGEGHERRYFYGSVWAVVIAQPILWLLWKVVPASRTGDALKLAVFVGVLAI
                     VGHMSRRGLLPRTRPIVPGEWAVSD"
BASE COUNT         1198 a         2459 c         2448 g         1257 t
ORIGIN      
        1 gccctcggca attactactg ggcgcttggc cgcctcaccg aaaccgagca gacgtttcgg
       61 agcgcgttga aggtcaagcc acacgacccg tcggcgaatc gtgcgatggc ggccctttcc
      121 atgacgacag gacgaaccgc cgaggccgag caatacctcc gaacgatcgc cgacacattc
      181 ggcgattccg cgtcggtggt cgcgctcgcg aactattacc tgcttgccgg ccggagcaag
      241 gacgcgatcg acaagctgac gccgctcgcc accggcaagg agaaagttcc gggaacgccg
      301 cagcttctgg cccgtgcgta cgcgctctcg ggcgacagcg gcaaggctcg ggagctcgtg
      361 gacacgattc tcgaggccga gcgcggggat ctggatgcgc aactcctcaa ggcgcagctc
      421 ctgtaccagg acggcaagcg agatgcggct ctcgcgctcg tggcagcggc ggccagcgcg
      481 aacccgacgt cggcggaagc gcagttgatg ctcggcaagc ttcacgtggc tcgcggcgat
      541 gtcgcgccgg ccgaggaggc ccttcgagaa gtgctcaggc tcaatccgcg cgtgacggca
      601 gcgcaggtcg agctgtcgag actgctgctc tcgacaggcc gcacggccga ctcgctccgc
      661 accgccgaag aagctgcgcg caacgagccc cgcagccccg aggctcgcct ggcgctggtg
      721 cgcagtctcc tggtggcgca ggacgcggcc agggcccaga gggagatcgc gaccctgctc
      781 gctgatcatc cggaagtcgc cgaggtacag gcgatggccg gagtcctcgc ggtcctgaag
      841 aacgacgtcg cgggcggccg ggcggcgttc gaacgcgcgc tatcggcgaa cgccaactcg
      901 ctcgaagcac tctccggcct cgtcgcgctc gatctgcgcg cgaaggacac tgccggcgcc
      961 atgaaacgga tcgacagcta cgtggcctcg aacccaagac ccgaggccat gctgctggct
     1021 gcccgcacgc acgcctccgc cggcgacacg gccggggcgg aacgctacct gcgccgcgcc
     1081 attgaagcgg atgcgtcgat gcttcccgcc tacgcgatgc tgggggagtt gtacctgtcg
     1141 cagaaaaggc tggacgaagc gcggaaagag ttcgacagcc ttgcgacgcg tcagaccaag
     1201 ccgatcgccg cgttgacgat gagcggcatc atcctcatga cccagggtca aaacgcgcag
     1261 gcccgagagc gattcgagaa ggcgctcgcg atcgatccgc gtgctcccgt cgccgccaat
     1321 aatctcgcct ggatgcaggt tgaagcgggc gagaatcttg atgtggcgct tcagctcgcc
     1381 caggcggcgg cgtccgcctc gcccgacact cccgagatca cggacacgct gggatgggtc
     1441 tactacaaga agaaccttcc ccagcaggcc attccccagt tcgagcggag cctgtccaag
     1501 gcgcccggga acccgatgtt tcactaccac ctcggactgg cgtatctgca ggccggcgac
     1561 gccgaccgcg gtcgagcgtc cctgcggcgg gccctttcag cgggcgctga cgccgggacg
     1621 gcggcagaga tcaagcgggt gctctcggca ccccaggccg cgtcggcgaa gaagtaagct
     1681 ccggctcacc ctcgttctat ttaccgaaaa gctccaacag gcgcctcgac ccctcgttga
     1741 tcaacccgcg cttgatcgcg gctgacgcgt caatcatcac gcgcggctta gcgagcgttc
     1801 cgctgattct cgcgggcagc accacgcggt tgccctcgcg cgtatagcgc acaaggtcgg
     1861 ttcccgcctg cgacgtcagc tcctccgaga gaaccatgtt gaccctcccg tcgagcgcct
     1921 cgttgccgag cgtcagcgtc cccgtgccgg tcagatcggc gtcccgcgag cggagcgaca
     1981 acgagtccgc acggaagact tggttggcga gcgagaacga ggcgtcgaat cgctcgaacc
     2041 ggtcgctcgc ggcgggtgtc tcagcggccg gttttccgaa gaacaagacg accgtccgca
     2101 cgagatcgag ccggcggatc gatccgtcca cgatcgaggc agtgcccttg ccgttcgcgc
     2161 tccccatgat ggcgtccatg tcggctccct gggagccgaa ggtaccgttg gccgtcaggc
     2221 gtccggtgac cgtgtcgggc gagcctccga atgccgccag ctgcgccacg tccacgtcct
     2281 ctacgcggga ctgcagcgtc gccgtgagtc tctccccgag tctggcgttc agcgatccct
     2341 cgtagcgccc gccgaagatc tgaaacgcga tcggcgtcag cgcgatccgg ttgccatcga
     2401 cctccatgcc ggtcgagaac tgcgtcacct cgaggtctcc gaccttcgcc gtcttcgact
     2461 cgatgcgcgc ggcgattcgc gcgggtgtcg agacctgctt gcgcgacgcg ggtgctccgg
     2521 ctggtgtaaa cgcggccgcc agtgccagca gctcgtcgac gtcgatcttg ttggcggtgg
     2581 ccttcaactg cgcgtcgatg cgcggctcga gatccacctt gccgctgacc tcgagggcgg
     2641 tgcctccgga ttgagcggag aaccggcgca cgacgagcgt gtcgccttcg agcgtcgaat
     2701 cggccgacac gacgacctcg cgtccgcggc tcaacacacg gatgtttcgg agcgcgatct
     2761 caccgatcga cacgaatttc accgctggct gagatgcgcc ggtggacttg ccgcgcgatc
     2821 caccgctcga tccactgccg gagggaatgc cgaatggaat cggcatctcg agccggctgt
     2881 ccgagatgac gatgcgcgca tcctcgatgc gcctgtccag cagcgcgcgc aggtcggtcg
     2941 agagctgaac gccggacagc gtgacgcgcg ccggctctcc gagccgtacg tcgctcagcc
     3001 ggatgccgat tctggggaag atctgcccgc gcgctccgcc gatgcgcacg ggatgtccca
     3061 gccacgacgt ggcctggctc tcgagggcca gccgcatgcc gtctcccgag aagagccaga
     3121 acactccggc cgctgcggcc aggagaagaa cgacgatacc ggcggccgtg atgagaagga
     3181 ttttgcggat catggcatca gcctactcct ccttttcggc ctcgagcgtc tcgcacagca
     3241 gcggctcagc agtagacttc gaggagcgtg cccgagctgc cagacatcct tctttatctc
     3301 cacgcgcttc gatcccgagt cgtggggaag cggatcgaga cggttcggct cgtgtcgccg
     3361 ttcctgcttc gctcgatcga tccacctctg tccgccgtcg aagggcgtgt cgtcaccgat
     3421 ctgcaccggc ttggaaaacg catcgcgatc gagacggagc gagaactgtt tctcgtcttt
     3481 catctcatga ttgccggccg tttccggtgg aagccggccg gcacgcgagt gccgggcaag
     3541 gtcggcctgc tcgcgctcga cttcgagcac ggctcgctga tactgacgga ggcaggtacc
     3601 aagcggcagg cctcattgca cgtcgtcgat ggggccgacg cgctcgcgcg gcacaatcct
     3661 ggcggactcg aagtgatgga aaccggcgcc gagccgttcg agcaggcgtt gcggcgcgag
     3721 aaccatacgc tgaagcgcgc gctcaccgat ccgcggatct tcagcggaat cgggaatgcc
     3781 tattcggacg agatcctcca cgccgcgcgt ctgtcgccct tcaagcagac gtccacgctg
     3841 agccaggagg aagcgcaccg cctttatgat gcaacgcggg gaacgctcgc cgcatggacc
     3901 gagcggctgc aatcggaaac aggcgacggg tttccggaga aggtgacggc gtttcgagag
     3961 gggatggcgg ttcacggccg cttcggcaag ccgtgcccgg tctgcggctc ggccgtgcag
     4021 cgaatcgtgt acgcgtcgaa cgaggccaac tattgcgtca cgtgtcagac aggcgggcgg
     4081 ttgctggccg acaggtcttt gtcccggctg ctccgcgagg actggccgcg cacgctcgag
     4141 gatctcgagc ggcgaaagcg gggagacggg tgaccgcatg aggcgaccca cgctgaccgc
     4201 gatcgtgctg tgtctctgtg tcggcgcagc cgccgcaggg caggaacgga gcgagttgcg
     4261 ctggggaggc gacgccgagg gcggcgctcc gtttgtcgag gcggacccga acgacctgac
     4321 tcgcgttgtc ggattcgaag tggagatcgc ccagctgctg gcgagcggcc tcggccgcgc
     4381 tccgcggttc gtgcagagcg gcttcacgag tctcgatgca gccgcagccc gcggcgactt
     4441 cgacgtcggc ctgagcggca tcgaggacag cccggcccgc cgatcccggc tggcagtcac
     4501 gattccctac taccagtttc gcgaagtcct cacggtgcgc gagggggacg cgggcaggtt
     4561 tcgcgggctc ggcgacctcg ccggccggcg cgtggcgacc ctaggcgcca ccctcgccta
     4621 cgacttgctc ctcgccgcgc aaacgaagtt tggagtcgtc ccggtcacgt acgaggacga
     4681 tgtccatccg tataccgacc tcgtcttggg acgcgtggac gccgtcctgc tcgacgaggt
     4741 gctcgcgcgg cgcggcatcc gccggaaccc cgggctgacc aatcagccga ccgacgtcgg
     4801 agtcggttac tacgtcggca tcaccgcgcc agccaatgcg ccgctgcgcg atcggatgaa
     4861 cgagatcctg ctccaggcca tggccgacgg ccggctcgag gcgatcttcc ggcgatggag
     4921 tatgtggaac gacgaccagc cgcagctgta cgcgaggctg gctcgcggag gtcaaggcga
     4981 gatcgcacag gcgccgagtc cgcagcagcc cagcacgttc caggctgctg ttcgctatct
     5041 gccctcgctg ttccgcgccg ccgcgatcac gctcgtgctg tcgtgccttt cgatgagtct
     5101 ggctgtagcg ctgggcctgg cgatcgcgag cggcagggtc tacggcccgc cggcggttcg
     5161 aatgctgctg acaggctggg tcgagctcat tcgaggcacg ccgctcctcc tgcagctgtt
     5221 cgtgctctat ttcgggctgg ccgcgatcgt gcagcttccc gcgttcctgg cggcgctcat
     5281 cggtctcggc ttgaactacg cggcctacga gagcgagatc tatcgcggcg ccctcgaagc
     5341 cgtgcctgcg ggtcagctcg acgcggcccg cacgctggga ttcacggaga ggcaaacgct
     5401 ggtgctcgta cgggggcctc aagcctttcg tctcgcgctg gcaccgatga ccaacgactt
     5461 cgtggccctc ctcaaggact cttccctcgt gtcggtcatc acggtcgtgg agctgacgaa
     5521 gcagacgtca atcctcgccg ccaacatcgg cagctgggtg atcccgggca tcttgtgcgc
     5581 ggcgatgtac ctcgtgctct cgctcccgct ggcaagggcc gctcgacgtt tggagggcaa
     5641 gtggaagcgg taaggtgcga cggtacgacg gtacgagggt gcgaaggtgc aaaagtgaat
     5701 aatgcgacat ccatactcga cgtgtcggct ctgaccatcg tgcgtggagc caggacgctt
     5761 gcggacgacg tgtcgttcag cgccacacgc ggagaggtcg tcgtcctcag gggtccgtcc
     5821 ggcgccggga agacgacggt cctgcgcgcg attgccggtc tcgatccgat cgcggccggg
     5881 cgcatcaccg ttgacggcgt cgcgatcggt ccgggccgga ctccgcgcgc gcgagcgctg
     5941 cgcgagctcc accggcgcgt cgggatggtc ttccagttcc atcacctgtt cgagcacctc
     6001 accgcgttcg agaacgtgtg gctcgctccc gtccacgtgc tccggcagcc aagggccgag
     6061 gccgagcgcc gggcgcacga gcttctcgaa cagctgggca ttggcgctcg cgctcgagcg
     6121 ctgccgcgcg agctgtcggg cggcgaggcc cagcgcgtgg cgatcgcacg agcgctcgcg
     6181 gttggaccgc aggtgctcct gatggacgag ccgacggcgt cgctcgacac cgagcgtcgc
     6241 gacagtctcg ctggcacgct gcggcaacta gccgccggcg gctgcactct cgtgctcgcg
     6301 agccacgacg aggagttcat tcgcgcctgc tcgcatcgcg tcattctcat gcaggccggc
     6361 cgcgtcgtcg gaccctcctg atagaatccc tcgtccatgc ccgggccgca gatccccacc
     6421 gactccctgt tcgtcgagct cgacaccgat cgcaagattc gccaccgcaa caagctgtac
     6481 taccggttca accattggcc gatctgggtg ttcgtgttct tcattgcacc tggaccgctg
     6541 accttcgacc tgttcgagcg cgggttcgac agtcgattgg tggtctggct cggcatcgtg
     6601 ctggtcggca cggccgtcgc cgggctgctg ggaaagttgc caggctgtga accccgcccg
     6661 tacatcattc gcttcaccga ggaccggccg aacccgctct accggcgcat ctgctacacg
     6721 acggcctggg gtgaggtcgt ggcgttcgcg gtgctgaaca tcgcggggtt ggcctatgcg
     6781 gttgcgacgg gacagtggcg cctcaggcag atgtacgaag ttgcgtattt cccgctggcg
     6841 ggcaccgtct gggttctcgg cgcactcggt cagttaccgc gcgtgaaggc gtccacaaaa
     6901 ggcgaaggcc acgagcggcg ctatttctac gggtcggtgt gggccgtcgt gattgcccag
     6961 ccaatcctgt ggctgctgtg gaaagtggtg cccgcgtcgc gaaccggcga cgcgctcaag
     7021 cttgccgtgt tcgtcggcgt gctcgcgatc gtcggtcaca tgtcgcggcg cggccttctg
     7081 cctcgcacgc gcccgatcgt ccccggcgag tgggcagtgt ccgactgacg ccgtcactgc
     7141 ggctttagcg acagggatcg acagatctga gagcacctgt cacgtttcag aacggcacac
     7201 cccggtacgc acagtttcgt cagtcagaac gcacacctct gcgcgaaagt tcataattgg
     7261 ccgcgcgtca ccggccccct attctggatc caaggaaggt gacgtatgtc tcgtgagctt
     7321 tcgtttgtca ctcgctgcgt cggaacggtc gtgtgcgcgc tg
//
LOCUS       DBNGSH010000513         3705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3784438, whole genome shotgun sequence.
ACCESSION   DBNGSH010000513 DBNGSH010000000
VERSION     DBNGSH010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3784438"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC16CY_11770"
     CDS             <1..228
                     /locus_tag="AC16CY_11770"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354516.1"
                     /translation="GPGGQPQRWSLEATSRAALERMGVTADYVKAGDIIKARCHPLRD
                     GSRGCLLGFLKARDGSVKDWDGNNLPIPSDF"
     gene            313..1245
                     /locus_tag="AC16CY_11775"
     CDS             313..1245
                     /locus_tag="AC16CY_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3354517.1"
                     /translation="MTRFLLYGVLVACVLGAAVTAAQQPILRGGGAIVRVFVTVTDRD
                     GRLATTLTQNDFEVRDEGKAQPITLFDNSPQPIRMIVMLDVSGSMMGNMPLLRASSEQ
                     LFVRLRQDDLAKVGVFGHQVTISPTFTRNSRELLAALPQGIAEDAPTPLWRGIDDAMK
                     AFGDVTDARKVVVVLSDGRDSGPMFRQPYVSQVEVIERARANDVMVYAIGMRSRMVGG
                     NLSNPFQTLSSDLPDPGLAKVAEESGGGFLEISPRQDLGAAFAQVADELHSQYLLGFV
                     PPKPDGKVHDLDVRVVGQKGMKPRGRKSYVAPKE"
     gene            1254..2021
                     /gene="tpiA"
                     /locus_tag="AC16CY_11780"
     CDS             1254..2021
                     /gene="tpiA"
                     /locus_tag="AC16CY_11780"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR3354518.1"
                     /translation="MRTPFVCANWKMHKTVADAVKFVKELRGLAKDVADVEIVVAPAF
                     TAIHAAAEAARNSNVAIAAQDLHWEREGALTGEVSGAMLREAGAEYAIVGHSERRTLA
                     GETDAMVNRKTAAAFATGLIPIVCIGETLDQRERRETFDVLDRQITQGLDGLTSEHLA
                     QLVLAYEPVWAIGTGRNATPAQAAEAHGHIRQRLRQWFGGDAAEGCHIIYGGSVKPEN
                     IGDLASQSDVDGALVGGASLDVRAFFEIVSRSRPAAV"
     gene            2109..2447
                     /gene="secG"
                     /locus_tag="AC16CY_11785"
     CDS             2109..2447
                     /gene="secG"
                     /locus_tag="AC16CY_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR3354519.1"
                     /translation="MLYYVVAALFVLNCFVLMLVILLQQGKGGDIASAFGGGSSQAAF
                     GARSGATVLSRATTVCAVLFVVGAMALGILGQRGPGSVIGGRSQRGPTSAPAAPAPAV
                     PAPAAPAPAK"
     gene            2519..2605
                     /locus_tag="AC16CY_11790"
     tRNA            2519..2605
                     /locus_tag="AC16CY_11790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2553..2555,aa:Leu,seq:gag)
     gene            3014..>3705
                     /locus_tag="AC16CY_11795"
     CDS             3014..>3705
                     /locus_tag="AC16CY_11795"
                     /inference="COORDINATES: protein motif:HMM:NF021665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HIR3354520.1"
                     /translation="MLLVGAFAVVHAQEGKSTAAKPARWSDPATWPDRKVPGADGAVT
                     IGRDMNVVLDVSPPALRSLTIEGKLSFADNRDLELTTEWIMLHGELEVGTEAKPHARK
                     ATITFTDNVKGENIMAGMGDRGIMISGGTLNLHGDRTHTWSKLSATANAGATSIQVLD
                     ARGWTVGDEIVLASTDFDPRQAERRTIAAIRGNTITLDRKLDFMHFGKITFGVDERGE
                     VGLLTRNIKIQAS"
BASE COUNT          674 a         1175 c         1268 g          588 t
ORIGIN      
        1 ggcccggggg ggcagccgca gcgatggtcg ctcgaggcca ccagccgggc cgcgctggag
       61 cgcatgggcg tgacagccga ctacgtcaag gccggcgata taatcaaggc tcggtgtcac
      121 ccgcttcggg atggctcgcg cggctgcctg ctcgggttcc tgaaggcgag ggatggatcg
      181 gtgaaggact gggacgggaa caacctgccg atcccgagcg atttctaagt cggagacacg
      241 tcgcccagga ccaggcacca agcacgaagg accaaggacc aagctgccag ccgcctgcca
      301 cgagggagtt gcatgacccg gtttctgctt tacggagttc tcgtcgcgtg cgtgctcggc
      361 gccgccgtca cggccgcgca gcagccgatc ctcagaggcg ggggcgcgat cgtccgcgtc
      421 ttcgtcacgg tgaccgatcg cgacggccgc ctcgcgacca ccctcacgca gaacgacttc
      481 gaggtgcgcg acgaggggaa ggcgcagccg atcacgctct tcgacaacag ccctcagccg
      541 atccgcatga tcgtcatgct ggacgtctcg ggcagcatga tgggcaacat gccgctgctg
      601 cgcgcctcat ccgagcagtt gttcgtgcgc ctgcgccagg acgacctcgc gaaggtcggc
      661 gtcttcggcc accaggtcac gatcagcccc acgttcacga gaaattcgcg ggagctgttg
      721 gcggcgctgc cgcaaggaat cgccgaggac gcgcccacgc cactctggcg cgggatcgac
      781 gatgcgatga aggcgtttgg cgacgtgacg gacgcgcgga aagtcgtggt cgtgctgagc
      841 gacggcaggg acagcgggcc gatgttccgc cagccatacg tcagccaggt cgaggtgatc
      901 gaacgcgcgc gcgccaacga cgtgatggtg tatgcgatcg ggatgcgcag ccgcatggtg
      961 ggtgggaacc tgtcgaatcc gttccagacg ctgtcgtcgg acctccccga cccgggcctc
     1021 gcgaaggttg ccgaggagag cggcggcggc ttcctggaga tcagccccag gcaggatctc
     1081 ggcgccgcct tcgcgcaggt ggccgacgag ctgcacagcc agtacctgct cgggttcgtt
     1141 ccgcccaaac ccgacggcaa ggtgcacgac ctcgacgttc gcgtcgtcgg ccagaagggc
     1201 atgaagccgc ggggccgcaa gagctacgtg gcgcccaagg agtagcgggg gccgtgagaa
     1261 cgcccttcgt ctgcgccaac tggaagatgc acaagacggt cgccgacgcg gtgaagttcg
     1321 tcaaggagct tcgcgggctg gcgaaggacg tggccgacgt cgagatcgtc gtcgcgcccg
     1381 cgttcaccgc gattcacgcg gcggccgaag cggcgcgcaa cagcaacgtg gcaatcgccg
     1441 ctcaggatct gcactgggaa cgggaaggcg cgctcaccgg tgaagtgagc ggggcgatgc
     1501 tgcgtgaggc aggggccgag tacgcgatcg tcggacactc cgagcggcgg acgcttgcgg
     1561 gagagaccga cgccatggtg aaccgcaaga ccgcggccgc cttcgccacc ggcctcatcc
     1621 ccatcgtgtg catcggcgag acgctcgatc agcgcgagcg ccgcgagacg ttcgacgtgc
     1681 tcgatcgaca gatcacacag ggcctggacg ggttgacctc ggaacacctg gcgcagctcg
     1741 tgctggcgta cgagccggtg tgggcgatcg ggaccggtcg caatgccacg cccgcccagg
     1801 cggccgaggc ccacgggcac atccggcagc gcctgcgcca gtggttcggc ggcgacgcgg
     1861 ccgaggggtg ccacatcatc tacggcggga gcgtcaagcc cgagaacatc ggcgacctcg
     1921 cctcgcagag cgacgtggac ggggcgctgg tcggcggagc gagcctggac gtgcgggcat
     1981 ttttcgagat cgtctcgaga agccggccag cggctgtgtg aacggtgtga ctcgtaactc
     2041 aagactaaaa actcaagact caaaactaca caaggtataa tcaggttttg cgttttcgag
     2101 ggattcagat gctgtactac gtagtcgcgg cgctcttcgt gctcaactgc ttcgtgctga
     2161 tgctcgtcat cctgctgcag caggggaagg gcggcgacat cgccagcgcg ttcggcggcg
     2221 ggtcgagcca ggcggcgttc ggcgcacgga gcggggcgac ggtgctgtcg cgggcgacga
     2281 cggtgtgcgc ggtgctgttc gtggtggggg cgatggcgct cgggattctg ggccagcgcg
     2341 ggcccgggtc ggtcatcggc gggcggtcgc agcgcgggcc cacgtcagcg cctgccgcac
     2401 ctgcgccagc ggtgccggct cccgcggctc cagctccagc gaagtagatc gtacgtagtg
     2461 tccggcttcc ttgggcgccg aagcgcgaaa cgcgaaggcg gcagccggac ccctccacgc
     2521 ggaagtggcg gaattggcag acgcaccagc ttgaggggct ggcggtagca ataccgtggg
     2581 ggttcgagtc cccccttccg caccacattt acaatcaaca agttgcaaga aagtgaggcc
     2641 gccagacatg gcggcctctg ttgtcatgtt gtcaagactg ttgtcaacat gtcagcaatc
     2701 ttcggcttcc ggtcgatact ccaacaggtc gagcgctctt cggacgcacg agcgacgagg
     2761 ctcggcgaca atcacctcat ccgatgtcga gaaaactaga ctaggaaagc tctcggacga
     2821 cgagagcggg tcgcgctgga atgaatggat cgaacgcttg ggcgcgagct gcactacttg
     2881 tacggcgccc gccgccctgc ccgattctca cggtatgctg tgccgctgcg gttcttggcc
     2941 atcttcgagt gcggaggttg attccgtgag taagcgacat cgctcgttcc ttcttgcttc
     3001 gctccttcca gcgttgctgc tcgtcggcgc cttcgctgtc gttcacgccc aggagggaaa
     3061 atcgacggcg gccaagccgg cgcgctggtc tgatccggcc acctggcctg accggaaggt
     3121 accaggcgca gacggcgcag tcaccatcgg ccgtgacatg aacgtcgtcc tcgatgtcag
     3181 cccgccggcg ctgcgcagtt tgacgatcga gggcaaactg agcttcgccg acaatcggga
     3241 cctcgaactg accaccgagt ggatcatgct gcacggggag ttggaggttg gcaccgaagc
     3301 caagccccac gcccgcaagg ccaccatcac cttcaccgac aacgtgaagg gcgagaacat
     3361 catggccggc atgggcgacc gcggcatcat gatttcgggc ggcacgctca acctgcatgg
     3421 ggatcgcacc cacacctggt ccaagctgtc cgcgacggcc aacgccggcg ccacctcgat
     3481 ccaggtgctg gatgccaggg gctggacagt cggcgacgag atcgtcctcg cctcgaccga
     3541 tttcgatccc cgccaggccg agcggcgcac gattgccgcc atcagaggca acacgatcac
     3601 gctcgatcgc aagctggact tcatgcactt cggcaagatc accttcggcg tcgatgaacg
     3661 cggcgaagtc ggattgctga cgcgcaacat caagatccag gcatc
//
LOCUS       DBNGSH010000514         1930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2086924, whole genome shotgun sequence.
ACCESSION   DBNGSH010000514 DBNGSH010000000
VERSION     DBNGSH010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2086924"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="AC16CY_11800"
     CDS             complement(<1..1178)
                     /locus_tag="AC16CY_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3354521.1"
                     /translation="MPHHPKPPIPLDQRLAAYGALLQRVETLRDCPTQEDFLDRLSAH
                     VRELVPFDAMSLFQYESADQMLVLKLMEPQGMGRRMTAVPMDFGPGGRAISSQRPVVL
                     RSDGGPAPPIAVIAFRAGFKVLAAVPASTPEAHWGVLVIASRDLDDYPAELLAVVQHV
                     GAAAARTLSVLPSDERSRVTSIDTSEFGGRTRLLFSITSAVMLHRSVPDVLLGISKLL
                     RETIPHEWASIAIWDDAVQGLRRWAAVQPVATELIPEGEILDKTEPPCLAFDTGDVIE
                     IPHESLSRLRTKLTGALIARGVQSGVCLPLKTPRGKYGVLSVGSPRPDAFPTAEILLL
                     WQIAQQLALAIENATYFERVERYRREAADERDRFKLLLNVNNALVSHLSAHSLWSAVL
                     "
     gene            1481..>1930
                     /locus_tag="AC16CY_11805"
     CDS             1481..>1930
                     /locus_tag="AC16CY_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3354522.1"
                     /translation="MHARVRRPQSADDAGAGDAAGSSQGASILPFEGSVDSRERFFRA
                     LVEQTRDLITVVDRNGTIMFKNRPTEETLGYTTSELVGRNVFEFIHPDDEPQVRRAFE
                     QAHDGVEVPAIEHRFRNKDGSWRVFESIGRYSRDFPFGPIGIVNSRDV"
BASE COUNT          340 a          639 c          637 g          314 t
ORIGIN      
        1 tcgagcaccg cggaccagag cgaatgcgcg ctgaggtgcg acaccagcgc gttgttgacg
       61 ttgaggagca gcttgaaccg gtctcgctcg tctgccgcct cgcggcggta ccgctccacc
      121 cgctcgaagt acgtcgcgtt ctcgatcgcg agcgcgagct gctgcgcgat ctgccacagg
      181 aggaggatct cagcggtcgg gaaggcgtcc ggcctggggc tgccgaccga gagcacgccg
      241 tacttgcccc ggggcgtctt gagcgggagg cacacgcccg actgcacgcc gcgggcgatg
      301 agggcacctg tcagtttggt acgcaggcgg ctgagcgatt cgtgcggtat ctcgatcacg
      361 tccccggtgt cgaatgcgag gcaagggggc tcggtcttgt cgaggatttc accttccgga
      421 atgagttccg tcgcgaccgg ctgaaccgcc gcccaccggc gcaatccctg cactgcgtcg
      481 tcccagatcg cgatgctcgc ccactcgtgc gggatcgtct cgcgcagcag cttcgagatg
      541 ccgagcaaca catccggaac actccgatgc agcatgaccg cgctggtgat gctgaacagc
      601 aacctcgtgc ggccaccgaa ctcggaggtg tcgatgctcg tgacgcggct ccgctcgtcg
      661 gacggcagga cgctgagtgt gcgcgccgcc gcggccccga cgtgctgcac caccgccagc
      721 agctccgccg ggtagtcatc caaatcgcgg ctggcgatga ccagaacccc ccaatgcgct
      781 tcgggcgtcg atgccggcac ggcggcgagc accttgaaac ccgcgcggaa ggcgatcacg
      841 gcgatcggcg gggcgggtcc gccgtccgag cgcaggacga cgggtcgttg gctggagatg
      901 gcacgtcctc caggcccgaa gtccatgggc acggcggtca tccgccggcc catcccctgc
      961 ggctccatca gcttcagcac gagcatctgg tctgccgact cgtactgaaa gagtgacatc
     1021 gcgtcgaacg gcacgagctc gcgcacgtgc gcggaaaggc gatcgaggaa gtcttcctgg
     1081 gtcgggcagt cgcgaagcgt ctcgacgcgc tgcaggagcg cgccgtacgc cgccagacgt
     1141 tgatcgagtg gtatcggggg cttgggatgg tgaggcattg cagcccctga ggcccatgtt
     1201 tgaaccccga agtgcccgct gtcaagtcag gacgtgacga tcgtcatgtg acgacatgcc
     1261 gtcgctcgac cgaagtgggg acgaatcgac gacacctcgg ccattcgacg aaacccaggc
     1321 gttacaagtg tttccggaga atcaacggaa tggctgaaac gggcacggat cctgcgatga
     1381 acggccgatt ggaatgcact gttcgtcggg gccactacac gaaagggtcg tgtccgcctc
     1441 cttactccct ccccgcaccg agccagtacc aaagggttct atgcacgcac gtgtacgccg
     1501 tccgcaatcc gccgacgacg ccggcgcagg tgatgccgcc gggtcctccc agggcgcgtc
     1561 gattctgccg ttcgagggaa gcgtcgacag ccgtgaacgc ttctttcggg cgctcgtgga
     1621 gcagacgcgc gatctgatca ccgtcgtcga tcgcaacggc acgatcatgt tcaagaaccg
     1681 accgaccgag gagaccctcg gctacacgac gtccgagctc gtcggccgca atgtcttcga
     1741 gttcatccac cccgacgacg agccgcaggt ccgccgcgcc ttcgagcagg cgcacgacgg
     1801 cgtcgaggtg ccggccatcg agcatcgctt ccggaacaag gacgggagct ggcgcgtgtt
     1861 cgagtcgatc ggccggtact cgcgcgactt cccgttcgga ccgatcggca tcgtcaactc
     1921 ccgcgacgtc
//
LOCUS       DBNGSH010000515         1682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1916702, whole genome shotgun sequence.
ACCESSION   DBNGSH010000515 DBNGSH010000000
VERSION     DBNGSH010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1916702"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC16CY_11810"
     CDS             complement(<1..257)
                     /locus_tag="AC16CY_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354523.1"
                     /translation="MAWFVLGRVLFTAAVVYSAYQLRPLNGGTAPNLVFGAVLGLLIV
                     VFELRLKQISVTHLVGALVGGAVGLLAAKTLGAALYWANLS"
     gene            complement(330..>1682)
                     /gene="radA"
                     /locus_tag="AC16CY_11815"
     CDS             complement(330..>1682)
                     /gene="radA"
                     /locus_tag="AC16CY_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR3354524.1"
                     /translation="CQECGAQAPKWMGRCSECGAWNSLVEERAAEPGSAGTNRFAQFS
                     TTSAAKLYAEVDIAQGVRLSTGIGEFDRVLGGGVVPGSLILLGGEPGIGKSTLLLQAA
                     AFFAREVGPVLYASGEESEHQIKSRGDRLGVGEAPLYLLSETCIERILEEAARLKPAL
                     VVVDSVQTVWSAKFQSAPGSIGQVREAATQFLFAAKSQNVPTVLVGHVTKDGSLAGPK
                     VLEHVVDTVLYFEGERHHAHRVVRAVKNRFGAVSELGVFEMTGSGLLPVPNPSKLFLS
                     ERASGAPGSAVLCCVEGSRPILVEVQALVSTSSYGNAKRMAIGIDQNRLSLLLAVLEK
                     RAGLSLAGDDVYVNIAGGMSVDEPAADLSVVAAVASSVRNRSLPPATAVLGEVGLSGE
                     IRGVPQAGLRVREAAQMGFTRIVLPAANLDRSGQDPGAERCELVGVRTVGEALDQLIA
                     "
BASE COUNT          295 a          601 c          545 g          241 t
ORIGIN      
        1 tcgctcaggt tggcccagta gagggcggcg ccgagcgtct tcgcggcgag caatccgacc
       61 gccccgccca ccagcgcgcc aaccaggtga gtgaccgaga tctgcttgag gcggagctcg
      121 aagacgacaa tcaggaggcc gaggaccgcg ccaaaaacaa ggttgggggc ggttcctccg
      181 ttgagcgggc gaagctgata cgcggagtag acgacggccg cggtaaacag aacacgtccg
      241 agcacgaacc aagccatgat cactccagcg ccagtacgat ccagccgccg atatgaatgc
      301 tggaacctta aaaccgctgg cgctgaaaat caagcgatca actggtcgag cgcctcgccc
      361 actgtccgga cccctaccag ctcgcatcgc tccgcaccgg ggtcctggcc ggaccgatcg
      421 agattggccg ccgggagcac gatccgggtg aaacccatct gcgccgcctc gcggacgcgc
      481 agccccgcct gcggaacgcc ccgaatctcc ccgctcaggc cgacctcgcc cagcaccgcg
      541 gtcgccggcg gaaggcttct gttgcggacg ctcgacgcaa ccgccgccac cacgctcagg
      601 tccgcggccg gctcgtcaac gctcatgccg cccgcgatgt tcacgtagac gtcgtctccc
      661 gcaagcgaga ggccggcccg cttctcgagg accgccagca gcagcgaaag gcggttctgg
      721 tcgatgccga tcgccatccg cttcgcattg ccgtacgagc tcgtgctcac gagcgcctgc
      781 acctccacga gaatgggccg cgacccttcg acgcagcaca gcacggcgga accaggcgcg
      841 ccggaggcgc gctcggagag gaaaagcttc gacgggttcg ggacgggcag gaggccgctg
      901 ccggtcatct cgaagacgcc gagctcgctg acggcgccga aacggttctt cacggccctg
      961 accacgcggt gagcatggtg ccgctcgccc tcgaagtaga gcaccgtgtc cacgacgtgc
     1021 tcgagcacct tcggccccgc aaggctgccg tccttggtga cgtggccgac gaggacggtg
     1081 ggcacgttct ggctcttggc cgcgaacagg aactgggtgg ccgcttcgcg gacctggccg
     1141 atgctgcctg gcgcggactg gaacttggcg gaccagaccg tctgcacgga atcgacgacg
     1201 acgagagcgg gcttgaggcg cgccgcttcc tcgaggatgc gttcgatgca cgtctccgac
     1261 agcaggtaga gcggcgcctc gccgacgccg agccgatcgc cccggctctt gatctggtgc
     1321 tcggattctt cgcccgatgc gtagagcacc ggaccgacct cccgggcgaa gaacgcggcg
     1381 gcctgcagca gcagcgtgga cttgccgatg ccgggctccc ctccgagcag gatgagcgag
     1441 ccgggcacca ctcctccgcc cagcacgcgg tcgaactcgc cgatccccgt agacagccgc
     1501 acgccctgcg cgatgtcaac ttctgcgtac aacttcgcgg cggatgtcgt cgaaaactgc
     1561 gcgaaccggt tggtgccggc gctcccgggc tcggccgccc tttcctcgac gagcgagttc
     1621 caggctccgc actcgctgca gcggcccatc cacttgggcg cctgcgcgcc gcactcctgg
     1681 ca
//
LOCUS       DBNGSH010000516         1702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3784783, whole genome shotgun sequence.
ACCESSION   DBNGSH010000516 DBNGSH010000000
VERSION     DBNGSH010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3784783"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="AC16CY_11820"
     CDS             <1..913
                     /locus_tag="AC16CY_11820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3354525.1"
                     /translation="EVPAIEHRFRNKDGSWRVFESIGRYSRDFPFGPIGIVNSRDVTE
                     RKSIEAQFRRAQRMEVLGRMASTVAHDFRNVLAVINASADRLLEGDPSPNVRRRATEL
                     VDAVQHADAWTRRLLMFSRGEAVAAPQVVSVNQVVIELAGFLHQLVGTNIGLALALGA
                     SSSWITIDRVLLEQVIVNLVLNARDAMPSGGVVTIGTRNVERPDAAGPELPCVAIEVT
                     DTGVGMTQEVRERIFEPFFTTKGSEKGSGLGLSAVFSVVHQAGGRIDVETQQGRGTTF
                     RVTLPAHPRRREGERTCNNPSGSPRAW"
     gene            919..>1702
                     /locus_tag="AC16CY_11825"
     CDS             919..>1702
                     /locus_tag="AC16CY_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3354526.1"
                     /translation="MHDHEHVLVVENDERFAVDMVGTVLELGFTASSAASASQAMELA
                     QRSRPSVLVADLDLGGELGGVRLAQTVTSRWGSAVILMTTRTDRETAAAIAEVESTDV
                     LCRPFHWRQFEMTLRLALERRARAASSPAGPTLAVVPHETSRLEAELTLRRIAAELSR
                     AGYGPPVPGISPHHEWLAAMRPREREVVKLLLQHQRVPAIARTLSIRPATVRNHLKNV
                     FRRMGIHSQQELLLLLQQTPDQSGGANRPTLAEVPKPNGTDED"
BASE COUNT          275 a          566 c          608 g          253 t
ORIGIN      
        1 cgaggtgccg gccatcgagc atcgcttccg gaacaaggac gggagctggc gcgtgttcga
       61 gtcgatcggc cggtactcgc gcgacttccc gttcggaccg atcggcatcg tcaactcccg
      121 cgacgtcacg gagcgcaagt cgatcgaggc gcagttccgg cgggcgcagc gcatggaagt
      181 gctcggccgg atggcgagca ccgtagcgca cgacttccgc aacgtgctgg cggtgatcaa
      241 cgcctccgcc gaccggctgc tcgagggcga cccctcgccg aacgtccggc ggcgcgcgac
      301 cgagctggtc gacgccgtgc agcatgccga cgcgtggacg cgccggctgc tgatgttttc
      361 gcgcggcgag gccgtcgcgg cgccgcaggt cgtcagcgtc aatcaggtcg tcatcgagct
      421 ggcggggttt cttcaccagc tcgtcgggac caacatcggc ctcgcgctcg cgctgggcgc
      481 gtcctcctcg tggatcacca tcgaccgggt gctgctcgag caggtgatcg tgaacctcgt
      541 cctcaacgcg cgcgacgcga tgccgtcggg cggcgtcgtg acgatcggca ccaggaacgt
      601 ggagcggccg gatgccgccg gtccggagct cccgtgcgtc gcgatcgagg tgaccgacac
      661 gggggtcggc atgacgcagg aagtcaggga gcggatcttc gagcccttct tcaccacgaa
      721 gggctcggaa aaaggcagcg ggcttgggct gtcggcagtg ttcagcgtcg tccaccaggc
      781 cggagggcgc atcgacgtag aaacgcagca ggggcgcggg accacattcc gggtcacgct
      841 ccctgctcat ccacgcaggc gagaagggga aagaacatgc aacaacccca gcggctcacc
      901 gcgagcatgg taagggaaat gcacgatcac gaacatgtgt tggtagtcga aaacgacgag
      961 cggtttgccg tcgacatggt gggcaccgtc ctcgaactcg ggttcacggc gtcctcggcg
     1021 gcgtcggcgt cgcaggccat ggagctcgcg cagcgctcgc gtccgtcggt gctcgtcgcc
     1081 gatctcgacc tgggcggcga actggggggt gtgcgtctcg cgcagaccgt caccagccgg
     1141 tggggctcgg cggtgatcct gatgaccacg cgtacggatc gcgagacggc ggcggcgatc
     1201 gccgaggtcg agtccaccga cgtgctgtgc cggccgttcc actggcgcca gttcgagatg
     1261 acgctgcggc tcgcgctgga gcggcgcgcg cgtgcggcca gctcgccggc cgggcccacc
     1321 ctggcggtgg tccctcacga gacgtcgcgg ctcgaggcgg aactcacgct caggcggatt
     1381 gcggcggagc tgtcgcgcgc cggctacggt ccgccggtcc ccggcatctc gccgcaccac
     1441 gagtggctgg cggcgatgcg cccgcgcgag cgggaagtcg tcaagctcct gctccagcac
     1501 cagcgcgtgc cggccatcgc gcgcacgctg tcgatcaggc cggccaccgt gcgcaatcac
     1561 ttgaagaacg tcttcaggcg gatgggcatc cactcgcagc aggaactcct gctcctgctg
     1621 cagcagacgc cggatcagtc gggcggagcg aatcggccaa ctcttgccga agtgccgaag
     1681 ccgaacggta cggacgaaga ca
//
LOCUS       DBNGSH010000517         1866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4634299, whole genome shotgun sequence.
ACCESSION   DBNGSH010000517 DBNGSH010000000
VERSION     DBNGSH010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4634299"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="AC16CY_11830"
     CDS             complement(<1..316)
                     /locus_tag="AC16CY_11830"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354527.1"
                     /translation="MRQNLRTAALCALVGGVVALAPTRGLAHHSATMFEEKRTITVEG
                     VVKEFQYTNPHSWLLVEVKDKTGKVTTWGFEAEGPSTLQRAGIRPSDFRPGTKLTITG
                     RPM"
     gene            complement(316..1245)
                     /locus_tag="AC16CY_11835"
     CDS             complement(316..1245)
                     /locus_tag="AC16CY_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354528.1"
                     /translation="MTKTRVLAVAPVLLAALTAACSGTGSAPQPAQSQQRERSKEEKI
                     ELGRQHKTAADLYQALRAEAKGGQPLALTALPDWSGVYSRRPAAGFAFDQDQQPGSLP
                     TAKLTPEYQAKMMQRIEAVKKGIEWDPISTCAPPGHPRWLTEPFLREFIVTPNQTWLI
                     NEMVNDVRRIYTDGRDHPPEADRYPLYNGDSIGFWDGDRLVIHTNQLMAGIYQRSHPD
                     YTEQVETVEIWHKADAKTLVADVWVYDPPALAEPWYTLQSYVKLDDPDRSLRIRYWHC
                     GENQNNSVVQTGTGSTRFQDFTFTRQDDKQGGK"
     gene            complement(1386..>1866)
                     /locus_tag="AC16CY_11840"
     CDS             complement(1386..>1866)
                     /locus_tag="AC16CY_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354529.1"
                     /translation="PMLPEPYNNNYRIIQSPGHVTILVEMNHDARIIPLDRRPHLSSA
                     VQQWTGDSRGRFEGNTLIVETRNLKFNEKSRFGVGYLNGLSDENLRVVERFTRTDANT
                     LTYQATIEDPTVFVKPWTVEISMDRTEGPLYEVACHEGNYGMFNILSGHRAEERRAH"
BASE COUNT          307 a          590 c          589 g          380 t
ORIGIN      
        1 tcatcggccg gcccgtgatc gtcagcttcg ttccggggcg gaagtcactg gggcggatgc
       61 cggcgcgctg aagcgtgctg gggccttcgg cctcgaatcc ccaggtcgtg accttgccgg
      121 tcttgtcctt gacctcgacg agcagccacg agtgggggtt ggtgtactga aactccttca
      181 ccacgccctc gacggtaatc gtccttttct cctcgaacat ggtggcggaa tgatgcgcca
      241 ggccacgtgt tggcgccaag gccaccacgc cacccacgag cgcgcagagg gcggccgtgc
      301 gtaggttctg cctcactact tgcctccttg cttgtcgtcc tgcctggtga acgtgaagtc
      361 ctgaaaccgg gtcgagccgg ttccagtctg gacgaccgag ttgttctggt tttcgccgca
      421 gtgccagtac cgtatacgca aactgcggtc cggatcgtcc agcttcacgt aggactgcag
      481 cgtgtaccac ggctcggcga gtgcgggagg atcgtagacc cagacatcgg ccacgagcgt
      541 cttggcgtcg gccttgtgcc agatctccac cgtttccacc tgctcggtgt agtcaggatg
      601 cgatcgctgg taaatgccgg ccatcaactg attcgtgtga atcacgagcc ggtcgccgtc
      661 ccagaagccg atcgaatcgc cgttgtagag cgggtaccga tcggcctcgg gcggatgatc
      721 gcgcccgtcg gtgtagattc tccggacgtc gttgaccatc tcgttgatca gccacgtctg
      781 attcggggtg acgatgaact cgcgcagaaa cggctcggtc agccacctcg ggtgtccggg
      841 cggagcgcac gtggagatgg gatcccactc gatgcccttc ttcactgcct cgatccgctg
      901 catcatcttc gcctggtatt caggcgtgag cttggcagtc ggcaggctgc ccggctgttg
      961 atcctgatcg aatgcgaaac cggcggcggg ccgccggctg tacacgccgc tccagtcggg
     1021 cagtgccgtc aaggccagag gctgcccgcc cttcgcctca gcgcgcaacg cctgatagag
     1081 atcagcagcg gtcttgtgct ggcggcccag ctcgatcttc tcttccttgg agcgctcgcg
     1141 ctgctgcgat tgagcaggct gcggggcgga gccggtcccc gaacacgccg cggtgagcgc
     1201 cgccagcagc accggggcaa cagcgagcac tcgtgtcttc gtcatcctga cgccctccgt
     1261 cgcgaggccg aaactatttt agcggactgt atcgactgga agagtacccc gaacaacgag
     1321 cttcgaccgc gattctccgg ccgcgacaac ggagcggccg gcaataatcc gcgaaggaca
     1381 taccactagt gggctcgccg ttcctcggcc cgatgccccg agaggatgtt gaacatcccg
     1441 tagttgcctt cgtggcacgc gacctcgtag agcggacctt ctgtccgatc catcgagatc
     1501 tcgaccgtcc agggcttcac gaacacggtg ggatcctcga tcgtcgcctg ataggtcagc
     1561 gtgttcgcat cggtccgcgt gaagcgctcg acgacgcgca ggttttcgtc gctcaggccg
     1621 ttgagatagc ccacaccgaa gcggctcttc tcgttgaact tcagattgcg cgtctcgacg
     1681 atcagtgtgt ttccctcgaa ccggccgcgt gagtcgccag tccactgctg caccgcagac
     1741 gacaggtgcg gacgtcggtc gagcgggatg atccgggcgt cgtggttcat ctcgacgagg
     1801 atcgtcacgt ggcccggact ctggatgatg cggtagttgt tgttgtaggg ctccggcagc
     1861 atcggc
//
LOCUS       DBNGSH010000518        20131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2087186, whole genome shotgun sequence.
ACCESSION   DBNGSH010000518 DBNGSH010000000
VERSION     DBNGSH010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 20131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20131
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2087186"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="AC16CY_11845"
     CDS             complement(<1..179)
                     /locus_tag="AC16CY_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HIR3354530.1"
                     /translation="MPPVLDRDLVLNALRVVIDPDIRKDIVSLGFVKDLAVAGSRVSF
                     TIELTTPACPVKDQMR"
     gene            complement(191..571)
                     /locus_tag="AC16CY_11850"
     CDS             complement(191..571)
                     /locus_tag="AC16CY_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354531.1"
                     /translation="MERHVRLLGILAGLWGALSILFGLSMLLLAAGALVELGESLGTS
                     LEFAAGLTAAVFATIGAFALLWGGAHVWASALLRRRQPLGRILCLGLALVNLLVLPFG
                     TALGVYALWVLLTNEGRRLFEPTK"
     gene            complement(575..1030)
                     /locus_tag="AC16CY_11855"
     CDS             complement(575..1030)
                     /locus_tag="AC16CY_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354532.1"
                     /translation="MSREPFGRHGVNRVAIVPVDGDHVEARCFADEVVIDFPSVALAV
                     DRIRRGFLADESPARWKTTVRLSGEEARRGSTVPLEVPVRLTCHHCGGRGESWTEQCR
                     DCMGSGSRLMHHVLQVSIPAGVLNGDRFRFTVVPRWHAPTPIELQVLVG"
     gene            1072..1245
                     /locus_tag="AC16CY_11860"
     CDS             1072..1245
                     /locus_tag="AC16CY_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354533.1"
                     /translation="MAGKECPMCGDVMQLREHEVTDRVPGTLQTRTSKAMEWVCPECD
                     YFEDWESDDDEGE"
     gene            complement(1258..1479)
                     /locus_tag="AC16CY_11865"
     CDS             complement(1258..1479)
                     /locus_tag="AC16CY_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354534.1"
                     /translation="MALTCPSCGNDQNFLVKTLQMHVVHQDQSRLEASEEGRPAVLEV
                     QCDECETALNFDEIDEGIRRELLLTLGAR"
     gene            complement(1673..5038)
                     /locus_tag="AC16CY_11870"
     CDS             complement(1673..5038)
                     /locus_tag="AC16CY_11870"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3354535.1"
                     /translation="MTDLPLPFEEEPGASALLDARDRAGRERAVDPRFNVALEASAGT
                     GKTRVLVDRYVNLLRAGVDPSEILALTFTRKAAAEMRDRIVATLRDAAAKGEIPPARW
                     RELRDRTADVAISTIDAFCLSLLREFPLEADLDPGFQMADETEVPRLVDEALDRALRI
                     CREVAREDEHVALVFAQLGDRRARAGLSVLLGRRIVAPAVLARYLSLGPPDLTVQEAA
                     RRGAAALAGVFVSMRGGLDRFLDTGPLHAKFSLFARELRRLEAGIEAGAVEPAFVQSA
                     FVHAREHFLTQEGDPRKVLPQRYGTSAFASAADRHAHREFVVGHARAIADAYAAYRRD
                     LNVLVSRGVWRMFMIAQAEYRRTLEARAALDFPDLLLKSLDLLRQMEEFAQSRFRLES
                     RYHHVLVDEFQDTNRAQWELVSLLIQSWGEGAGLAHTGSLAPTVFIVGDRKQSIYAFR
                     DADVSLFRDASRYMSGLRPGGDVRRSISRSFRSVPPLLAFVNDLCAAIAKSPGRRDAF
                     AYDEQDRFPVDEAAEPSGDAISLIAAGTLESCARMTADEIGRLMAEGALVRDRDTGVR
                     RPMRPADVAILFRTRESHREYEAALEQRGISAYVYKGLGFFEADEIQDVLALLRHLAD
                     PASDLSAAAWLRSRFIRMSDEGLRRLGPRFADALASTAPPRADLDASDTEALLMASRA
                     SVRWCSLADRLPPAELLDRAIQETAYMAELRGRRLQQGRENLKKIRAYIRRIQNRGYA
                     TLERIVTHLERLAVGDEANAVIDAGDAVSLMTVHAAKGLEFPVVFLVNLARGTGNRRD
                     SIRVAVESDRDVSVAVGDFESPADEDAAARDREESKRLLYVALTRARDRLYLGSVVKD
                     GRFQPGRGSLGEVMPDSLQALFAEAARGTAVLYWAHASSSSHAIRVCTPASVVEAPEP
                     RAADAATAARVRDFAVLEDDSVPRIAASTQADTVEDEAARPAPPKPGAKAGPSNGSGS
                     HRLIGLVVHRLLQHEGLVPDAPDERLRRLALDAMRGQSVEEVDLASLAAEAIARFRAL
                     SAQRDLRETYLSGQPFHEVPFVLTVGDRIVRGAIDCLVAAPDRVTVLEFKTGRPRDGD
                     RAQTELYRLAAQALFPDALVETRLVYTSASRTS"
     gene            complement(5031..7898)
                     /locus_tag="AC16CY_11875"
     CDS             complement(5031..7898)
                     /locus_tag="AC16CY_11875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR3354536.1"
                     /translation="MPDLQSFREAVVDLAIAGPPLDARDRIVVVPTHAAAAHLVRSIE
                     DEVLHPGRSAVVLPDLVTSRELTLRFSQRLREPPRLLTDAEREALLGVACRAARDRGD
                     EPPFRLRPGLIAAILHFYDELRRRQNGVDDFERRVLDLLEPGAATDRGAERLVRQTRF
                     LVAAFREFETRSRDYGVDEHGLREHTTDTASPRPFRHVVLAVADEAFDPYGLHPAHWD
                     FLTRVPGLTRLDVVVTDVMLAGALHERIHRMLPGIEEVRYQRREPPVRPALLVPPRGG
                     LFHVARDREEEVAAVARRVKADVRQGALSSPGRAALVVRQRLPYVYVAREVLRSGGVR
                     CQMFDALPLAGEPYAAALDLVLTCVGSDFARVPAVALLRSPHFRFATAHDASLLDRAL
                     AEAGYLGGITALERLLDRWRAERSQPALRSGELLLEVARQLVPLRSGAPVADHLGVLL
                     AFLAAHEAPPVADDDLRARHLRARGAIVATLGALRDGYASIDSAPVHGDEVAALVRRW
                     IEAQTFAPRSGESGVHLVDGDSARFGRFEYVHIAGVVDGEWPDRPRRSIFYSPAVLRE
                     LGWPSELDRLAGARGAFAELLGLPALRLSVSAFALEADALVSPSPLTDELDQAGLETV
                     EEAASSDRIFEYEALCGEPGDPALLALESRDWTDRRFRPIDLPPGRFHGFTMPPPARP
                     YSLSAFERYQDCPFKFFAADVLGLKEGPEDESTPSPRARGRFIHEVFERFFEAWDSRV
                     GGAITTERLDQARALLEEVAEPLLARLTEADAALERTRLLGSAISTGSFDVVAGHEAA
                     RPAEVQQRWLEHRFEGEFGLGFEDRRVALRGVADRVDLLRGNRLRLIDYKSGGAPNTS
                     RALQVPIYALCAQETLQARDGMPWAIDEAAYLALSAKRPYVPVVAAGGDRGEGLEAAR
                     RRLRTILDGIAGGQFPPRPHDRMICDYCAYPAVCRKDYVDD"
     gene            complement(7945..9321)
                     /locus_tag="AC16CY_11880"
     CDS             complement(7945..9321)
                     /locus_tag="AC16CY_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354537.1"
                     /translation="MSRAVRVFIVLACLILPQLVSAATTPTLFRLFLLDGSVLVSYGE
                     FVRIDDSVLFSMPIGGPSDAPRLQVATLRAELVDWPRTERYAASVRYQQYAQTRGEED
                     YQLLSNEVADALNAIAASPDRQQALMMAERVRQRVAEWPQSHFGYRHNDIREIVSVLD
                     QAISSLRAAPGNRFELSFVAMAGPPDLEPVLGMPNARQQLDQVLRLAKETKTPSDRVA
                     MLQAALMMIHESGSSIPAAEQAALRRTTERLIRVELDDDRNYANLSRRMMTEATSAAR
                     RADPAAVERLAARVPKEDAKLGRKRPLVVSALQSSMQARLADARHLRLLTDQWQLRRV
                     TYRQYQSSVGAELLQLVKSTPSLEAIRNLNGPETDQLIALQHRFTGGAERLERMRAPE
                     YLRSIHEQLIGAWRFAENAAKARFRAVSDADSAAAWEASSSAAGALLMLTRVQQDIKA
                     LLTPPKLQ"
     gene            9586..9804
                     /locus_tag="AC16CY_11885"
     CDS             9586..9804
                     /locus_tag="AC16CY_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354538.1"
                     /translation="MNTYHFEHDIAGRLYRIEVTPVGGRWRAQLRRAAGMATALMPFY
                     GSTPEEAASHMTDWLNLAHRRQPAQTAR"
     gene            9927..12302
                     /locus_tag="AC16CY_11890"
     CDS             9927..12302
                     /locus_tag="AC16CY_11890"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR3354539.1"
                     /translation="MSLKSCHGVGLALAALVFFLSACTRPEAPATTATGGGLDRTVLP
                     IHEPAVPAIKELDARNAAAPPRFDVKAPNGAPNVVIVLIDDIGFGSSSSFGGPINMPT
                     LEKMAGSGLRYNRFHTTALCSPTRVALLTGRNHHVNNAGAIMELATAFPGNTGIRPQS
                     VTPLAQILRMNGFSTAAFGKYHETLPWEVSVSGPYDRWPTGSGFDKFYGFIGGETNQW
                     APAIYDGVTRVEHDMSPNYHFTTDMTNKAIAWVSAQHSLTPDKPFYVYFATGATHAPH
                     HVPKEWIDKYRGKFAGGWDTLREETFERQKAMGIIPADAKLTPRPKEIPAWDGMSANQ
                     KRLFERQMETFAGFAEHTDHEVGRLVAQLEALGELDNTLFFYIVGDNGASAEGGPEGA
                     YNEMMALNGIVGTYDQMAVHIDDWGGPATFPHYAIGWAWASNTPFQWTKQIASHFGGT
                     RNGMVMQWPARIKAKGEIRSQFHHVIDVAPTALEAAGLPEPKMVNGVAQRPMDGVSML
                     YTVDDAKAADRRKTQYFEMFGNRAIYHEGWVAASRHSIPWLLVQNPPLANDVWELYDV
                     GTDFSQADDLAAKNPAKLKELQAVFMQEAERNHVLPIDDRRAERFDPKIAGRPDLLGG
                     RTTMTVFDGMTGIMENAFINVKGVRHSVTAEVTVPDSKTEGVIIAQAGYFGGWALYMK
                     AGRVVHDYNFFGLTHTKVASKSPLAAGRHTIVYEFVPDAAKPGTGGKSILRVDGQVVA
                     EGHIKQTQPFMFSADEGVDVGSDNETMVSDDYKPGATDFKGEIVKVIVSQL"
     gene            12374..12449
                     /locus_tag="AC16CY_11895"
     tRNA            12374..12449
                     /locus_tag="AC16CY_11895"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12407..12409,aa:Ala,seq:ggc)
     gene            complement(12688..13548)
                     /locus_tag="AC16CY_11900"
     CDS             complement(12688..13548)
                     /locus_tag="AC16CY_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354540.1"
                     /translation="MKARVSVAIAAVVLILTGAPAGSQTITPIEFPGAAQTRPSGINP
                     SGEITGNYVNPDGSIHGFLLDRNGFTSIDVPGAIQTNASGINPQGDIVGFYIPPGGRG
                     RGFLWSRGTFYDIFFPGAFLTSALGINAQGDIVGQYNDAAGPPRHGFLLRNNSFVAID
                     FPGAVRSVTVDINERGDIVGTYVDAGGKSYMYLLSSGVFTTIDFPGALGTSPAGGINA
                     KGEIVGSYLGTDLKTHGFLLNASGFSTIDLPETIVTDFTRPIGINAQGDIVGFYRDLA
                     NLAHGFLIRR"
     gene            13650..14291
                     /locus_tag="AC16CY_11905"
     CDS             13650..14291
                     /locus_tag="AC16CY_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3354541.1"
                     /translation="MGAERVRTPNRDALAEGGRVAPEAGVVTGLLRAWGEGDLQARDT
                     LMPLVYKELRRQATAYLRRERADHTLQPTALVHEAYLRLIGQKRVLWQNRAHFFGIAA
                     QMMRRILVDHAKGHRRGKRFGAAVKVMLDERVGAAGPPDVDLLLLDQALDELTAIDVR
                     QGKIVELRYFGGLSESEVAEVLAISRSTVTREWQIARGWLYRRVTTGRVQDRA"
     gene            14291..17200
                     /locus_tag="AC16CY_11910"
     CDS             14291..17200
                     /locus_tag="AC16CY_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3354542.1"
                     /translation="MANPSWARVKEVIEAALDRAPGERVEFVLRTCGEDGALRAEVQS
                     LLAAIEQAGTFIARPALQPLAPSARSSASAIPDSAPRALDPGDALGPYEVLEFLGAGG
                     MGEVYRAGDSNLHRDVALKVLPETFASDSDRLARFKREAHVLASLNHPNIAAIYGFEE
                     ADGVQALVLELVEGPTLAGRIAQGAIPVNEALSIAKQIAEGLKAAHERGVMHRDLKPS
                     NIKVRSDGAVKILDFGLAKALNPSDSGSAVPEVATITSPATTHAGAIFGTAAYMSPEH
                     ARGKPMDKRTDIWAFGCVLYETLSGRQAFRGETIQDTLAAVLGDAPDWNLLPADTPAS
                     VVRLLRRCLEKDPDRRLHDIADARIEIEDADSARPIAVLVKRARVAFWPLVAAAAIAI
                     VVAVWNWMRPASPTPAATQMVSRLLIGLPEAQPLARAPSMPLGLGQVSIAISPDGTRL
                     AYVMERQGVTQLYLRALDQLEPAAIPRTEGAFGPFFSPDGRWIGFFAGNKLKKVAASG
                     GAPIDLCAAPNPHGGSWGTNGTIVFAADEGRRPATIRDTGGVPQRVGVKDDRGSWRQP
                     HMLPGGKAAIVSHVGDVGVLWLETGEYRRLVENGSDGRYAPSGHLIFARAGALLAAPF
                     DLERMAVSGPATAVLDGVRAEGPVAQAVFSRDGTLIYVPGVANDATRPVWVDRQGKVQ
                     PVGMPPRSYRSFSLSPDGRRLAIAIGDGDPNNDIWVQDLERGTLTPLTSSGRNVQPKW
                     TPDGKRVVFIERTGGGATPFWVAADGSSEPERMFKGDHEGGVGSFSPKGDLVAFVRRA
                     PGTGLDLWVQTVHGAQTEQPFLRTRFTEGGPKFSPDGRWIAYGSDESGQYEVYVRPYP
                     PGPGEWQVSTQGGEHVIWSRDGKELFYRNGNKWMVVVVHLKPEFTPEAPRLLFEGPYV
                     MVGGQSYDVTPDGQRFLVLEPAEQEAAQVTHLNVVLNWFEEVKQKAGSGVASLPR"
     gene            17344..18657
                     /locus_tag="AC16CY_11915"
     CDS             17344..18657
                     /locus_tag="AC16CY_11915"
                     /inference="COORDINATES: protein motif:HMM:NF019421.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HIR3354543.1"
                     /translation="MLVYNHFMPSVSDELGRGVLHAADLRERLGVSPATLMRMVRDAG
                     PDVVRIGRGRATQYALRQQWPNLDSSRFPLFRITGTGAAVSAGELTTLAARQSVWMPA
                     GRVSVGLPIELADARPSGFLGRHFASTHADLRLPLRLTDWSDHHILLAMSRRGEDLPG
                     NLIVGEESFARWQALKAGAVTRNDYPSLANDTIAGRPPGSSAGGERPKFGVLVDGRHV
                     LVKFAARGGATDIVARRWCDLLILEGVALDVVGSRGISAAPTNVLETPSHWFLESERF
                     DRVGARGRRGVLSLAAIHDDAADPWARAATSLREAGRLTDEDARRLRWLDAFGALIGN
                     TDRHQYNILFFTEEGIPRLAPAFDQVSMLYAPTVDGQVPPRVFSVPNVTSDTLDVWDD
                     ARDAARQFWVRGSEDARVSDDVRAICASNAQLLARNGHPGSTSPS"
     gene            complement(18652..19110)
                     /locus_tag="AC16CY_11920"
     CDS             complement(18652..19110)
                     /locus_tag="AC16CY_11920"
                     /inference="COORDINATES: protein motif:HMM:NF046631.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6968 family protein"
                     /protein_id="HIR3354544.1"
                     /translation="MNKSFAAPPRIRRIAARRVFRAPSGKRVVVTLGVPQAVPGSDWG
                     CPLQITGLNTTWRRPKYVFGIDGLQALHLAMKCADTVLQSAKLKLEWLGQTEDLGMPK
                     FLPALPKPEQDRLEAMVEREATKFWRSVERARKAKNSQRRNGPRTADKTS"
     gene            19210..>20131
                     /locus_tag="AC16CY_11925"
     CDS             19210..>20131
                     /locus_tag="AC16CY_11925"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HIR3354545.1"
                     /translation="MTLTRTRWHATCLVALVALTAADIVLDGQSRTIGAFFDDFSAEW
                     MRRRPSQSTGSRYFAGPEQDRLDRQLTPETLEWRRETVAQARRGLTELASFDRSRMTD
                     DERVSADVMQWQLQIIVDGEPFSDHDFPLDQFAGTNVGIPNLLTVVHPIRTENDARNY
                     VARLREVDDRMAEASEEAARLLAKGIRPPRFILRSTIAQMQQFTGTPAGQNPLVATFV
                     DRMNGVQSLTASVRQQLRDEATKIVESEVYPAWRKAVSLLEAQLQSATDDAGLWRYKD
                     GAAVYAHRLKQFTTTNMTAAEIHELGLREVK"
BASE COUNT         3641 a         6502 c         6732 g         3256 t
ORIGIN      
        1 cgcatctgat ccttcacggg acacgcgggc gtcgtcagct cgatcgtgaa agagacgcgc
       61 gaccccgcga cggcaaggtc cttcacgaat cccagcgaga cgatgtcctt tcggatgtcg
      121 ggatcgatga cgactcgcag ggcgttgagg acaagatccc ggtccagcac agggggcata
      181 tcctccgatc ttacttcgtg ggctcgaaga gccgcctccc ctcgttggtc agcaataccc
      241 agagcgcgta gacgccgagg gccgtcccga acggaaggac caggaggttc acgagcgcga
      301 gaccgaggca caggatgcgg ccgagcggct ggcggcgccg gagcagtgcc gacgcccaca
      361 cgtgggcgcc gccccagagc aaggcgaagg cgccaatcgt cgcgaaaacg gccgcggtga
      421 ggccggccgc gaactcgagc gacgtgccga gcgactcgcc gagctcgacc agggccccgg
      481 cggcgagcag cagcatggac agtccgaaca ggatggacag cgcgccccac aggcccgcga
      541 gaatgcccag gagcctgacg tgccgctcca cggtctagcc gacgagaacc tgaagctcga
      601 taggcgtggg cgcgtgccag cgcgggacga ccgtgaagcg gaaccggtcg ccgttgagca
      661 ctcccgcggg gatgctgacc tgcaacacgt gatgcatgag acgcgatccg ctgcccatgc
      721 agtcgcggca ctgctcggtc cacgactctc cccgcccgcc acagtgatga cacgtgagcc
      781 ggacgggtac ctcgagcggc acggtcgagc cccggcgtgc ctcctctccc gacagacgca
      841 ccgtcgtctt ccacctcgcg ggcgactcgt cggcgagaaa cccgcggcga atccgatcga
      901 cggcgagtgc caccgaaggg aaatcgatca cgacctcatc ggcgaaacag cgcgcctcga
      961 cgtgatcgcc gtcgaccgga acgatcgcga cgcgattgac gccgtgacgg ccgaagggct
     1021 ctctgctcat cgaactgctg gtacgatagg agccactcga ggagatgtca aatggccggc
     1081 aaggagtgtc cgatgtgcgg cgacgtcatg caattgcggg aacacgaggt caccgatcgg
     1141 gtgccgggca cactccagac gaggacgtcg aaagcaatgg aatgggtgtg tcccgaatgc
     1201 gactacttcg aagattggga gagcgacgac gacgaaggtg agtaaggggc gggcgcctca
     1261 gcgggcgccg agcgtgagca gcagctctct ccggatgccc tcgtcgatct cgtcgaaatt
     1321 cagcgccgtt tcacactcgt cgcactgcac ttccaaaacg gccggacggc cctcctccga
     1381 cgcctcgagc ctcgactgat cttgatggac gacgtgcatc tgcagcgtct tcaccaggaa
     1441 gttctggtcg ttcccacagc tgggacaggt cagggccacg cgaatatcct ctttcgtcgt
     1501 caatctttgc tctccgaatc ctgattttat gagatggatt ccggcaagcg caatgcataa
     1561 aagcacttgc gccaagctgg accgctcggc tcccgacggc gccgggccca aaacaaacgg
     1621 aaaaacctgc gatcgtgtga aagcgggccc ttgcgcagaa tcgcgcacgg actcacgaag
     1681 tccgcgatgc ggaggtgtaa acgagccggg tttccaccag ggcatcggga aagagcgcct
     1741 gcgccgcgag ccgatagagc tcggtctggg cccgatcccc gtcgcgtggc ctgccggttt
     1801 tgaactccag gaccgtgacc cgatcggggg cggcgactag gcagtcgatc gcgccgcgga
     1861 cgattcgatc gccgacggtc agcacgaacg gaacctcgtg aaacggctga ccggacaggt
     1921 atgtctctct tagatctcgc tgcgcggaca gcgctcgaaa cctggcgatc gcttcagccg
     1981 caagagaggc gaggtctacc tcctcgacgc tttgcccgcg catggcgtcc agagccaacc
     2041 gccgcagcct ctcatccgga gcgtcaggaa cgagcccttc gtgctgcaga aggcgatgga
     2101 ccaccagccc gatcaaccga tgcgatccgg agccgttcga cggacctgct ttcgcgccag
     2161 gcttcggcgg ggcaggccga gccgcctcgt cctcgactgt atcggcctgc gtcgatgccg
     2221 caatccgtgg aaccgagtcg tcctcgagca cggcgaagtc gcgcacgcga gcagcagtgg
     2281 cggcgtctgc cgcccgaggc tccggcgcct cgacgacgct cgccggtgtg cagacgcgga
     2341 tcgcgtggct cgagctcgaa gcgtgggccc agtacaacac ggcggtgccg cgggcggcct
     2401 cggcgaacag tgcctgaagg gaatcgggca tcacctcgcc gaggctgccc cggcctggct
     2461 ggaagcggcc gtccttcacg accgatccga gatagagcct gtcgcgggcg cgggtgagtg
     2521 cgacgtagag aagccgcttg ctctcctcgc ggtcgcgcgc tgccgcatct tcgtcggcgg
     2581 gagactcgaa gtcgccgacg gccacggaca cgtcgcgatc gctctcgacc gccacgcgaa
     2641 tggaatcgcg gcggttgccg gtgcctcttg ccaggttgac gaggaacacg accgggaact
     2701 cgagaccttt cgcggcgtgc accgtcatca ggctcaccgc gtcacccgcg tcgatgacgg
     2761 cgttcgcctc gtcgcccacg gccaatcgct cgaggtgcgt gacgatccgc tcgagagtgg
     2821 cgtagccccg gttctgaatc cgccggatgt aggcacggat cttcttgagg ttctcgcgtc
     2881 cctgctggag gcggcggcct cggagttcgg ccatgtaggc cgtttcctgg atggcgcgat
     2941 cgagaagctc ggccggcggc agccgatccg ccagcgagca ccaccgcacc gatgcccggc
     3001 tggccatcaa cagcgcctct gtatcgctcg catcgaggtc cgcgcgaggc ggggccgttg
     3061 acgcgagggc gtcggcgaag cgagggccga gccgcctgag cccctcgtcg gacatcctga
     3121 tgaaacggga gcgcagccag gcggcagccg acaggtccga ggcggggtcg gccagatgcc
     3181 tcaacagcgc cagcacgtcc tggatctcgt cggcctcgaa aaagccgagg cccttgtaga
     3241 cgtaggcgga gatgccccgc tgctcgagcg cggcttcgta ctctcggtgg ctctcgcgcg
     3301 tcctgaacag gatggcgaca tcggcgggcc gcatgggccg cctgacgccg gtatcgcgat
     3361 cccggacgag cgcgccctca gccatcagcc tgccaatctc atccgcggtc atccgggcgc
     3421 acgactccag cgtgccggcg gcgatgagcg agatcgcgtc gcctgaaggc tccgccgctt
     3481 cgtcaaccgg gaagcgatcc tgttcgtcgt acgcaaacgc gtcgcgccgc ccgggtgact
     3541 tggcgatcgc cgcgcacaga tcgttgacga aggcaagaag cggcggcacc gatcggaagc
     3601 tgcgcgaaat cgaccgccgc acatcgcctc ccggcctgag cccgctcatg taccggctgg
     3661 cgtcgcggaa caacgagacg tccgcgtcgc ggaacgcgta gatcgactgc ttccgatcgc
     3721 cgacgatgaa gaccgtcggc gcgagcgatc cggtatgggc caacccggct ccctcacccc
     3781 atgactggat cagcagcgag acgagctccc actgcgcgcg gttcgtgtcc tggaattcgt
     3841 ccacgagcac gtggtggtag cgcgactcga ggcggaagcg gctctgcgcg aactcctcca
     3901 tctgccgaag cagatcgagc gacttgagca ggagatcggg gaaatcgagc gcggcgcgcg
     3961 cttcgagcgt cctccggtac tcggcctgcg caatcatgaa catgcgccag acacccctcg
     4021 aaacgaggac gttcagatcg cgccggtagg ccgcgtaggc gtcggcaatc gcccgcgcgt
     4081 gacccacgac gaattcgcgg tgcgcgtgac ggtccgctgc cgacgcaaac gccgacgtcc
     4141 cgtatcgctg cgggaggact ttccgcgggt cgccctcctg ggtcagaaag tgctcccgcg
     4201 cgtggacgaa cgcggactgt acgaacgcgg gctcgacagc cccggcctcg atgcccgcct
     4261 cgagacggcg aagctcgcgc gcgaacagcg agaacttcgc gtggaggggg ccggtgtcga
     4321 ggaagcggtc cagccctcca cgcatcgaga cgaacacgcc ggccagcgcc gcagccccgc
     4381 ggcgggcggc ctcctgcacg gtgagatcgg gcggaccaag agacaggtac cgcgccagga
     4441 cagcgggagc gacgatccgt cgtccgagca gtaccgacaa gcccgcgcgg gctcgccgat
     4501 cgccgagctg cgcgaacacc agggcgacgt gctcgtcctc gcgcgccacc tcgcggcaga
     4561 tgcgcagcgc ccgatcgagc gcctcgtcca ccagtcgcgg cacttcggtc tcgtcggcca
     4621 tctggaagcc gggatcgaga tcggcttcga gggggaattc gcgcagcagg gacaggcaga
     4681 acgcatcgat cgtgctgatc gccacgtccg ccgtccggtc gcgcagctcg cgccagcggg
     4741 caggcgggat ttctcccttc gcggcggcgt cgcggagcgt cgcgacgatg cggtcgcgca
     4801 tctcggcggc ggcctttcgt gtgaaggtca gcgccaggat ttccgaagga tcgacgcccg
     4861 cgcggagaag gttgacgtag cggtccacga gcacccgcgt ctttccggtg ccggccgacg
     4921 cttccagcgc cacgttgaag cgcggatcca ccgcccgctc gcgtcccgcc cggtcgcgcg
     4981 cgtcgaggag ggcgctcgcc ccgggctcct cctcgaaggg cagcggcagg tcagtcatcg
     5041 acgtagtcct tccggcacac ggccgggtag gcgcagtagt cgcaaatcat ccggtcgtgc
     5101 ggcctgggcg ggaactgccc accggcgatg ccgtcgagaa tcgtccgcag cctccggcgc
     5161 gcggcctcga gaccctcgcc tctatcgccc ccggccgcga caacgggaac gtaggggcgc
     5221 tttgcgctga gggccaggta cgccgcctcg tcgatcgccc acggcatgcc gtcgcgagcc
     5281 tgcagcgtct cctgcgcgca gagcgcgtag atcggcacct gcagcgcgcg cgacgtgttc
     5341 ggggccccgc ccgacttgta gtcgatgagg cgaagccggt tgccgcgaag aaggtccacg
     5401 cgatcggcca cgcccctgag cgcgacgcgc cggtcctcga acccgagacc gaactcccct
     5461 tcgaatcggt gctcgagcca gcgttgctga acctcggctg gacgggcggc ttcgtggccg
     5521 gcgacgacgt cgaaggaacc agtcgagatc gcggacccca ggagacgggt tcgctcgagc
     5581 gccgcatcgg cctccgtgag acgtgcgagc agcggctcgg ccacttcttc gagcagcgcc
     5641 cgcgcctggt cgagacgttc ggtcgtgatg gctcccccga cccggctgtc ccaggcctcg
     5701 aagaagcgtt cgaacacctc gtgaatgaac cggccgcgcg cgcgcggcga cggggtcgat
     5761 tcgtcttccg gcccttcctt caagccgagc acgtcggccg cgaagaactt gaacggacag
     5821 tcctggtagc gttcgaacgc actgagcgag tacggccgtg ccggaggagg catcgtgaag
     5881 ccgtgaaacc tcccgggtgg cagatcgatc ggccggaacc ggcggtcggt ccagtcacga
     5941 gattcgagcg cgagaagcgc cgggtccccg ggctcgccac acagcgcttc gtattcgaag
     6001 atgcgatcgc tcgaggcggc ttcttcgacg gtctcgagcc cggcctgatc gagttcatcc
     6061 gtcaacggag acgggctgac gagcgcatcg gcctcgaggg caaaagcgga gaccgacagc
     6121 cgcaatgccg gcagcccgag caactccgcg aacgccccac gcgcgccagc caggcggtcc
     6181 agctcggagg gccagcccag ctccctgagg acggccggcg aatagaagat gcttcgccgc
     6241 ggacgatcgg gccactcgcc gtccacgaca ccggcgatgt gaacgtattc gaatcgcccg
     6301 aagcgggcgc tgtcgccgtc gaccagatgc acgcccgact cgccgctgcg aggcgcgaag
     6361 gtctgcgctt caatccagcg gcgcacgagg gcggcgacct catcgccgtg aacgggcgcc
     6421 gaatcgatgc tggcataacc gtcgcgcaag gcgccaagcg tcgccacgat cgcgccacgg
     6481 gcgcgaaggt gcctggcgcg caggtcgtcg tcggccaccg gcggcgcttc gtgcgccgcg
     6541 agaaacgcga gcagcacgcc aaggtgatcg gccaccggcg cgccggatct gagcgggacc
     6601 agctgacgag ccacctcgag cagcagctct ccacttcgaa gagcgggctg cgatctctcc
     6661 gcgcgccatc gatcgagcag acgctcgagg gccgtgatgc cgcccaggta gcctgcctcg
     6721 gcgagcgcgc gatcgagcaa cgaggcgtca tgggccgtgg cgaaccggaa gtgcggcgat
     6781 cgaagcagcg ccacggcggg gacgcgcgcg aagtccgagc cgacgcacgt gaggaccagg
     6841 tcgagcgcgg ccgcatatgg ttcgcccgcc agcggcagcg cgtcgaacat ctggcagcgc
     6901 actccgcccg agcgcaggac ctcgcgtgca acgtagacgt acggcagccg ctgacgaacg
     6961 acgagcgccg cgcggcctgg agaagagagc gcgccttgcc ggacatccgc cttgacgcgc
     7021 ctcgcgactg cggccacctc ttcctcgcgg tcgcgcgcga cgtggaagag gccgccgcgc
     7081 ggaggaacca ggagcgcggg gcgcacgggc ggttctcgcc gttggtaacg aacctcttcg
     7141 atgccgggca gcatgcggtg gatgcgctcg tggagagcgc ccgcgagcat gacgtcggtg
     7201 accacgacat cgagccgcgt caggccggga acgcgcgtca ggaaatccca gtgggccgga
     7261 tgaagaccgt atggatcgaa ggcctcgtcg gcgacggcga gcaccacgtg ccggaacggg
     7321 cgcggggatg ctgtgtccgt cgtgtgctcg cgaagaccat gctcgtcgac gccgtaatcg
     7381 cgagagcgtg tctcgaactc ccggaacgcg gccacgagaa agcgcgtctg acggacgagc
     7441 cgctcggcgc cgcgatcggt ggcagcccct ggttcgagca ggtcgagtac ccggcgctcg
     7501 aagtcgtcca cgccgttctg gcggcgccgc agctcgtcgt agaagtgaag aatcgccgca
     7561 atcagacccg gccgcaggcg gaacggaggc tcgtcgccgc gatcacgggc ggcgcggcag
     7621 gcgacgccga ggagtgcctc ccgttcggcg tccgtcaaca gccgaggcgg ctcgcgaaga
     7681 cgctggctga accggagcgt cagctcgcgg gaggtgacca ggtcgggcag gacgacggcg
     7741 ctccggcccg gatgaagaac ctcgtcttcg atgctgcgaa ccaggtgcgc ggccgcggca
     7801 tgcgtaggga ccacgacgat ccggtcccgc gcgtcgagcg gcggcccggc gatggcgaga
     7861 tcgaccacgg cctcgcgaaa cgactgaaga tccggcacgc ggatcagacg cgtggtgcgg
     7921 ggtgttggag acagagacag aggtctactg caatttcggt ggcgtcagca atgccttgat
     7981 gtcctgctgc acgcgggtga gcatgagcag ggcgcccgcg gctgacgacg aagcctccca
     8041 cgcagccgcc gagtcggcgt ccgacacggc gcggaaccga gctttcgcgg cattctccgc
     8101 gaaacgccac gcgccgatca gctgctcatg gatcgaacgg aggtattccg gcgcgcgcat
     8161 gcgctcgaga cgttcggcgc cgccggtgaa ccggtgctgc agggcgatga gctgatccgt
     8221 ctcggggccg ttcaggttgc gaatcgcctc gagcgacggc gtggacttca cgagctgcag
     8281 cagctccgcg ccgaccgagc tctggtactg ccggtaggtg acccggcgca gctgccattg
     8341 atcggtgagc agacggaggt gccgtgcatc ggccagccgc gcctgcatcg atgactgcag
     8401 cgccgacaca accagcggac gcttccggcc cagcttggcg tcctccttcg gaacgcgcgc
     8461 ggcgaggcgc tcgacagcgg ccgggtcggc gcgccgggcc gcagacgtcg cctcggtcat
     8521 catgcgccgc gacagattgg cgtaattgcg gtcgtcgtcc agctcgacgc ggatcagccg
     8581 ctcggtcgtc ctgcgaagcg cggcctgctc ggcggcggga atcgacgagc cggactcgtg
     8641 gatcatcatc aacgcggctt gcagcatcgc tacgcgatcc gaaggggtct tcgtttcctt
     8701 cgcgaggcgc aacacctggt cgagctgctg gcgcgcgttg ggcatgccga gcacgggctc
     8761 caggtcgggc ggcccggcca tcgcgacaaa cgagagctcg aagcggtttc caggagcggc
     8821 cctcaagctc gagatggcct ggtcgaggac ggagacgatc tcgcgaatgt cgttgtggcg
     8881 gtagccgaag tgcgactgcg gccactcggc cacgcgctgc cggacgcgct cggccatcat
     8941 cagcgcctgc tgccgatcgg gagacgccgc gattgcgttc aacgcgtcgg cgacttcgtt
     9001 gctgagcagc tggtagtctt cctcgcccct cgtctgcgcg tactgctgat agcgcacgga
     9061 ggccgcgtag cgctcggtcc tcggccagtc caccagctcg gcccggagcg tcgccacctg
     9121 cagtcgaggc gcgtcggacg gtccgccgat gggcatcgag aagagcacgc tgtcgtcgat
     9181 tcggacgaac tccccgtagc tgaccaggac gctgccgtcg agcaggaaca gccggaagag
     9241 agtcggggtg gtcgccgccg acaccagctg cggcaggatc aggcacgcca ggacgatgaa
     9301 gactcttaca gcgcgagaca caaggaagac tctcacaaag cgagacacaa cgttgagacg
     9361 cgccattgta tccgctggct gggggggcag atcgaggcct atcttggcgc tgactgtgga
     9421 aaagttgtgc acgagccgtg caaaacctgt gtagaagctg acagccgaca ttttttttcg
     9481 tcggaatccg ccgacgatcg gtttatcgga cgctgtggat gcgggcttcg cgccgggctt
     9541 cgcttgaccc cgtttgacgc ccgcgtagaa tcggcgcttc cccccgtgaa cacttatcac
     9601 ttcgagcacg acatcgcggg ccgcctctac cggattgaag tgacgccggt tggcggccga
     9661 tggcgcgcgc agctgcggcg cgcggccggc atggcgaccg cgctgatgcc attctacggc
     9721 tccactcccg aagaagccgc cagccatatg accgactggc tcaacctggc gcatcgtcgt
     9781 cagccggcgc agaccgccag gtagacaact caagactcaa aactcaaaac tcaaaactca
     9841 aggctcgagc tcgcgcgaag ccctgcggtt ggcacaagat ggatcgttcc gtctcaaccg
     9901 actgacgctt gcttgccgga gaaacaatga gtctcaagtc ttgccacggt gtcggcctcg
     9961 cgctcgcggc gcttgtcttc ttcttgagtg cctgtactcg tccggaggcg ccggcgacaa
    10021 cggcgactgg tggtggactc gaccggacgg tcctgcccat tcacgaaccg gctgttccgg
    10081 caatcaagga actggacgcc cgcaacgccg cggcaccgcc ccgcttcgac gtgaaggcgc
    10141 caaacggcgc gccaaacgtc gtgatcgtgc tgatcgacga tatcggcttc gggtcctcga
    10201 gctcctttgg tggcccgatc aacatgccga ccctggagaa gatggccggc agcgggctgc
    10261 gctacaaccg cttccacacg acggcgctct gcagcccgac gcgcgtggcg ctcctcaccg
    10321 gacgcaacca tcacgtgaac aacgccggcg cgatcatgga gctggccacg gcgttcccgg
    10381 ggaataccgg catccggccg cagagcgtga cgccgcttgc gcagatcctg cgcatgaacg
    10441 gcttcagcac ggcggccttc gggaagtacc acgagacgct gccctgggag gtgtccgtct
    10501 ccggtcccta cgaccgctgg ccgaccggct ccggtttcga caagttctac ggcttcatcg
    10561 gcggcgagac caaccagtgg gcgccagcga tctacgacgg cgtcacccgc gtcgagcacg
    10621 acatgtcgcc gaactatcac ttcacgaccg acatgacgaa caaggcgatc gcatgggtga
    10681 gcgcccagca ttcgctgacg ccagacaagc cgttctacgt gtacttcgcg accggtgcga
    10741 cccacgcgcc gcaccacgtg ccgaaggagt ggatcgacaa gtacaggggc aagttcgcgg
    10801 gcggatggga cacgctccgc gaggaaacct ttgagcgtca gaaagccatg ggcattatcc
    10861 ctgccgacgc gaagctgacg ccgcggccca aggagattcc ggcctgggac ggtatgagcg
    10921 ccaaccagaa gcggctcttc gagcgccaga tggaaacctt tgcgggtttc gccgagcaca
    10981 cggatcatga ggtcgggcgg ctcgtcgcgc agctcgaggc gctcggcgag ctcgacaaca
    11041 cgctcttctt ctacatcgtc ggcgacaacg gcgcgagcgc cgagggcgga cccgagggcg
    11101 cgtacaacga gatgatggcg ctgaatggca tcgtgggcac ctacgatcag atggcggtgc
    11161 acattgacga ctggggaggc ccggcgacct tcccgcacta cgcgatcggc tgggcctggg
    11221 caagcaacac gccgttccag tggaccaagc agatcgcgag ccacttcggc ggcacgcgaa
    11281 acggcatggt gatgcagtgg ccggcgcgca tcaaggcgaa aggcgagatc cggagccagt
    11341 tccatcacgt gatagacgtg gcaccgacgg cgctcgaggc cgcgggcctg ccggaaccga
    11401 agatggtcaa cggtgtcgcg cagcgtccga tggacggcgt ctcgatgctg tatacggtgg
    11461 acgacgcaaa ggccgcggac cggcggaaaa cgcagtactt cgagatgttc ggcaaccgcg
    11521 ccatttatca cgagggctgg gtcgcggctt cgcgccattc gattccgtgg ctgctggtcc
    11581 agaacccgcc gctcgcaaac gacgtgtggg agctgtacga cgtcggcacg gacttcagcc
    11641 aggccgacga cctggctgcg aagaacccgg cgaagctcaa ggagctgcag gccgtgttca
    11701 tgcaggaggc cgagcgcaac cacgtgctgc cgatcgacga tcgacgcgcc gagcggttcg
    11761 atcccaaaat cgcgggccgg ccggacctcc tcggcgggcg cacgacgatg accgtgttcg
    11821 acggcatgac gggcatcatg gagaacgcct tcatcaacgt caagggcgtg cgccactccg
    11881 tgacggcgga ggtgacggtg ccggattcca aaaccgaggg cgtcatcatc gcgcaggccg
    11941 ggtacttcgg cggctgggcg ctctacatga aagccggccg cgtggtgcac gactacaact
    12001 tcttcggcct cacgcacacg aaggtcgcct cgaagtcgcc gctcgccgcg ggcagacaca
    12061 cgatcgtcta cgagttcgtg ccggacgcgg cgaagcccgg aacaggcggc aagtcaatcc
    12121 tgcgcgttga cggccaggtg gttgccgagg gccacatcaa gcagacgcag cccttcatgt
    12181 tctccgcgga cgagggtgtg gatgtcggct cggacaacga gaccatggtg tccgacgact
    12241 acaagcccgg cgccaccgac ttcaagggcg agattgtgaa ggtgatcgtc tcgcagctct
    12301 gacactggag cggccggatt ggaaaccgcg caggtccggc ttacaattga ggttcacacc
    12361 tgcacccaac gatgggcctg tagcgcagtt gggagcgcgc ctgaatggca ttcaggaggt
    12421 cacgggttcg actcccgtca ggtccaccat ccttcgctcg cctttcaggc gagcttcgga
    12481 tggcaagcca agcgagctaa tcgaccggcg aaggatgccc accgtagcgc aaagcgcgaa
    12541 ggtgggccta ctttagatcc gaacgagctt cggatggcaa gccacgactc gattacaggc
    12601 gaaggttgcc caccagaacc ttgggcccgc caagcgacat cggctcgaac tgacatacgt
    12661 ctccttaaca gcccaagagg tgggccgtca gcgacgaatc agaaagccgt gggcgaggtt
    12721 ggcgagatcg cgatagaaac ccacgatgtc cccttgtgcg ttgattccta tcgggcgggt
    12781 aaagtcggtc acgatcgtct caggaagatc gattgtgctg aagccgctcg cgttgagaag
    12841 aaagccatga gtcttgaggt cagtgcctag gtaggacccg acaatttccc ccttcgcgtt
    12901 gataccaccc gcggggctcg ttcccagcgc tccgggaaag tcgatcgtgg tgaatacacc
    12961 ggacgagagc aggtacatat aggacttgcc gcccgcatcg acataggtcc caacaatgtc
    13021 gccacgctcg ttgatgtcca cggtgaccga acggaccgcg cccggaaaat caatcgccac
    13081 gaaactgttg ttgcgcagaa gaaagccgtg ccgtgggggg ccggccgcgt cgttatactg
    13141 gccaacgata tctccctggg cattgatgcc cagcgcactt gtcaggaagg cccctggaaa
    13201 gaaaatgtcg tagaacgtgc ctcggctcca cagaaaaccg cgacccctgc cccccggggg
    13261 gatgtagaag ccgacgatgt ctccctgtgg gttgattccc gatgcattcg tttggatggc
    13321 gccgggaaca tcgatgctcg tgaatccatt gcggtcgagc aggaacccat gaatactgcc
    13381 gtcaggattc acgtagttgc ctgtgatctc cccgctagga ttgattccgc tgggcctggt
    13441 ttgcgcggcc cccgggaatt caatcggcgt tatcgtttgt gacccggcag gtgcgccggt
    13501 caggatcagc acgactgcgg caatcgcgac actcactctc gctttcatca cgtcctcctc
    13561 aggcactacc ttgtagccgg tggcagagat agaatcgacg ccaccatccc agtcgagagc
    13621 taacgcgaca agacggagcc aattgcgtca tgggggccga acgggtgcga acgccgaacc
    13681 gggacgcttt ggcagaaggg ggacgcgtcg cccctgaggc cggtgtggtc acgggattgc
    13741 tgcgcgcgtg gggcgaaggc gaccttcagg cgcgggacac actgatgccg ctggtctaca
    13801 aggaactgcg gcggcaggcg actgcctatc tccgccgcga acgggcggac cacacccttc
    13861 aaccgacggc actcgtccac gaggcatatc tgcgcttgat cgggcagaag cgcgtcctct
    13921 ggcagaatcg cgcacacttt ttcggaattg cggcgcagat gatgcgccgc atcctggtcg
    13981 atcacgcgaa aggacaccgg cgcggaaagc gcttcggtgc cgcggtgaag gtgatgttgg
    14041 atgaacgcgt gggcgcggcc ggacctccag acgtcgacct gctgctgctc gaccaggcgc
    14101 tcgatgagct cacggcgatc gacgtccgcc aaggcaagat cgtggagctc cgatatttcg
    14161 gcggtctgtc ggagagcgag gtcgccgagg tcctcgcgat ctcgcgatca acggtcacgc
    14221 gggaatggca gatcgcgaga ggatggctgt accgtcgagt gacgaccggc cgagtacagg
    14281 atcgggcgta atggccaatc cctcttgggc gcgcgtcaag gaagtgatcg aggctgccct
    14341 cgatcgggcg cccggtgagc gcgtcgagtt tgttctgcgg acgtgcggcg aggacggcgc
    14401 cttacgagcg gaggtacagt ccctgctcgc agcaatagag caggcgggca cctttatcgc
    14461 tcggccggcg cttcaacccc tcgcaccgtc agcccgctcg tccgccagcg cgatacccga
    14521 ctcggcgccg cgcgctctgg accctggcga tgctctcggt ccgtatgagg ttctcgagtt
    14581 tctcggcgcg ggcggcatgg gtgaggtcta tcgtgcaggt gattccaatc tccatcgcga
    14641 cgtggccctc aaggtcctgc ctgaaacctt cgcctctgat tccgatcgcc tggcccgttt
    14701 caagcgcgaa gcgcacgttc tcgcctcgtt gaaccacccg aacatcgcgg cgatctacgg
    14761 gttcgaggaa gctgatggcg tccaggcgct cgtgttggag ctggtcgagg gaccaacgct
    14821 ggccggtcgc attgcccagg gggccattcc ggtaaacgag gctctgtcaa tcgcgaaaca
    14881 gatcgctgaa gggttgaaag cggcgcacga gcgaggggtc atgcatcgcg acttgaaacc
    14941 ctcgaacatc aaagtgcgct ccgacggcgc ggtgaagatt ctggacttcg ggttggcgaa
    15001 agcgctcaac ccgtccgatt ccggttcagc cgttcccgag gtggccacaa tcacgagtcc
    15061 ggccacgact cacgcaggtg cgatctttgg caccgcggcg tacatgagcc cggaacatgc
    15121 gcgcggaaag ccgatggaca agcgcaccga catctgggcc ttcggttgcg tcctctacga
    15181 gaccctgagc gggcgacagg cctttcgcgg cgagaccatc caggacaccc tcgccgctgt
    15241 gctcggcgac gcgccggatt ggaacctgct gccggccgac actccagcga gcgtcgtgag
    15301 gctgttgcgc cgatgcctcg agaaggatcc cgaccgccgg ctgcacgaca ttgccgatgc
    15361 aaggattgag atcgaggacg ccgattcagc gagaccgatt gccgtcctcg tgaagcgcgc
    15421 ccgcgtggcg ttctggcctc tggttgcagc cgccgcgatc gcgatcgtcg tcgccgtctg
    15481 gaattggatg cgtccggcga gtccaacccc cgcggccaca cagatggtga gccgactcct
    15541 gatcgggctg cccgaggcac agccgctggc gcgcgcaccg tcgatgcctt tgggactcgg
    15601 gcaggtgtcg attgcgatct ctccggacgg gacacgcctg gcctatgtga tggaacgcca
    15661 gggcgtcaca cagctctatc tgcgcgcgtt ggaccagctg gagccggcgg cgatcccccg
    15721 gacggaaggc gcgttcggtc ccttcttctc tcctgatggc cggtggatcg gattcttcgc
    15781 cggcaacaag ctcaagaaag tcgctgcatc gggcggcgcc ccgattgatc tctgcgccgc
    15841 ccccaatccg cacgggggca gctggggaac caacggaacc atcgtctttg ccgccgacga
    15901 ggggcgtcgt ccagcaacga tccgagatac cggtggcgtc ccccagcgtg tcggtgtcaa
    15961 ggatgatcgt ggttcctgga ggcagccgca catgctgcct ggcgggaagg cggcgatcgt
    16021 ttcacatgtg ggtgacgttg gggtgctttg gctggaaacc ggcgagtacc gcaggctggt
    16081 cgaaaacggc agtgatgggc gctatgcgcc gagtggccac ctgatttttg cgcgcgcggg
    16141 cgcgctgctg gcggcgccgt tcgatctcga gcggatggcc gtgagtgggc cggcgacggc
    16201 ggtcctcgac ggggtcagag cggagggccc agtggcgcag gctgtctttt cgcgtgatgg
    16261 cacgttgatt tacgtcccgg gggtcgccaa cgacgcgacg aggccggtat gggtggatcg
    16321 tcaggggaag gtccaacccg ttggcatgcc gccgcggtcg tacagatcat tcagcctctc
    16381 gcccgacgga cgacgcctgg cgatcgcaat cggcgacggc gaccccaaca acgatatttg
    16441 ggttcaggat ctggaacgcg gtacgctgac gcccctcacg tcgagcggga gaaacgttca
    16501 accgaagtgg acacccgatg gtaagcgcgt ggtgttcatc gaacgcacgg gcggcggagc
    16561 gacgccgttc tgggtagcgg ccgatggcag cagtgaacct gaacggatgt tcaagggcga
    16621 ccacgaaggc ggagtggggt cgttctcgcc gaagggcgac ctcgtcgcat tcgtgagacg
    16681 ggccccaggc accggcctgg atctttgggt gcagaccgtg cacggggctc agaccgagca
    16741 gccgttcctg cgcacccgct tcacggaagg cggacccaag ttctctcccg acgggcgctg
    16801 gattgcctac ggctcggatg aatccggcca gtacgaggtc tacgtgcgcc catatccacc
    16861 gggtcccgga gagtggcagg tctcaacaca gggcggcgaa catgtgatct ggtcgcggga
    16921 cggcaaggaa ctcttctatc gaaacgggaa caagtggatg gtggtcgtgg ttcatctcaa
    16981 accggagttc acacctgaag cgccgcgcct gctgttcgaa ggaccctacg tcatggtcgg
    17041 tggtcagtca tacgacgtga cgcctgatgg ccagcggttc ctggtgctcg agcctgccga
    17101 gcaggaagcg gcacaggtga cacatctcaa cgtggtgctg aactggtttg aagaagtgaa
    17161 gcagaaggca ggctcaggcg tcgcttcctt gcctcggtga tccattggct gtcttcgcgc
    17221 ctgcggtgga tggcaactgg gctcatcata cagttcggcc ctagataatc gtctcctcaa
    17281 tcatttctat aatcgtttcg tacataggca cacaagccgt aattaggcca tactttggca
    17341 atcgtgctcg tctataatca tttcatgccg agcgtgtcag atgagctggg ccgtggagtc
    17401 ctccatgcgg ctgacctccg ggagcggttg ggcgtttcgc ccgcgacgct catgcggatg
    17461 gtgcgggatg ctggaccgga tgtcgtccgg attggtcgcg gccgcgccac gcagtatgcc
    17521 ctgcgacaac agtggcccaa tctcgacagt tcgcgctttc cactgttccg aatcaccggc
    17581 accggcgcgg cagtctcggc aggcgaactg acgacgcttg cggcgcggca gtcggtttgg
    17641 atgccggccg gcagggtctc agtcggcctt ccgatcgagc ttgccgacgc tcgcccttcg
    17701 ggattccttg ggcgacactt tgcctcgact catgccgacc ttcggctgcc gctgcgactg
    17761 accgactggt ctgatcatca catcctgctc gcgatgtcac gtcggggtga ggacctgcct
    17821 gggaacctca tcgtcggcga ggagtctttt gctcggtggc aagcgctaaa agctggcgcc
    17881 gtcacacgca acgattaccc ttcgctggcc aacgacacaa tcgctggacg tcctccaggt
    17941 tcgtcggcgg gaggcgagcg gccgaagttt ggtgtgctcg ttgacggacg gcacgtgctc
    18001 gtcaagtttg ctgcgcgcgg aggtgcgacc gacatcgttg cgcggcgttg gtgtgatctg
    18061 ctcatcctgg agggagttgc gcttgacgtt gtcggctcga gagggatctc ggctgcaccc
    18121 acgaacgttc tcgaaacgcc ttctcattgg tttctcgaga gcgagcgttt tgaccgtgtg
    18181 ggagcacgcg gaaggcgtgg cgtcttgagt cttgcggcta ttcacgacga cgccgctgac
    18241 ccctgggctc gtgccgcgac gtcgctcaga gaagctggcc gtctaaccga tgaagatgcg
    18301 cgacgactgc gctggctcga tgcgtttggc gcacttatcg gaaacaccga tcggcatcag
    18361 tacaacatcc tctttttcac ggaagagggc atcccgcgac tggcgccggc cttcgatcaa
    18421 gtctcgatgc tgtacgcgcc aaccgtggat ggtcaggtgc cacctcgcgt tttttcggta
    18481 ccgaacgtca catcggacac cctggatgtc tgggatgatg cgcgggacgc cgcgcgacag
    18541 ttctgggtcc ggggaagcga ggacgcacgt gtgtcagatg atgttcgggc catttgcgcg
    18601 agcaatgcgc agttgcttgc gaggaacggg caccctgggt ccacctcacc ttcatgaggt
    18661 cttgtcagcc gttcttggcc cattgcggcg ctgcgagttc ttcgccttac gagcgcgctc
    18721 gacactgcgc cagaacttgg tcgcttcgcg ctcgaccatc gcttcgagac gatcctgttc
    18781 tggcttcggt aacgctggca ggaacttggg catacccagg tcctcggtct gtcccaacca
    18841 ctccagcttc agcttcgcgg attggagcac cgtatcggca cacttcatgg cgaggtgaag
    18901 cgcttgaagc ccgtctatgc cgaacacgta tttgggcctg cgccaggtcg tgttcaggcc
    18961 ggtaatctgc agggggcagc cccagtccga accgggaacg gcctggggca cgccaagtgt
    19021 cacgacgaca cgcttgccac tgggcgccct gaaaacccgg cgcgcggcaa tccgacgaat
    19081 tctgggtggc gcagcgaagg atttgttcat accgccgagc gcgatcgtat caggccgcag
    19141 ccggctaggc ctctactcgg cgcgtcgcgc cctcgcgttc gcgtgccgtt cagggttagc
    19201 attcgcgacg tgaccctgac gcgaacacgt tggcacgcga cgtgcctcgt cgctctcgtc
    19261 gccctgaccg cggctgacat cgtcctcgac ggccagagcc ggacgatcgg cgcgttcttc
    19321 gacgactttt cggccgagtg gatgcgacga cggcccagtc agtccacggg cagccggtac
    19381 tttgccggac ctgagcagga tcgcctcgac cgccagctca cgccggaaac actggagtgg
    19441 agacgcgaga cggtggcgca ggcgcggcgc ggcttgacag agctcgccag cttcgaccgc
    19501 tcgcgcatga ccgacgacga gcgcgtgtcg gccgacgtca tgcagtggca gctgcagatc
    19561 atcgtggacg gcgaaccgtt cagcgaccac gacttccctc tcgaccagtt cgccgggacg
    19621 aacgtgggga tcccgaatct gctgacggtc gtgcacccga tccgcaccga gaacgacgcg
    19681 cgcaactacg tggcgcggct gcgtgaggtg gacgaccgga tggcggaagc cagcgaggaa
    19741 gccgcgcgcc tcctggcaaa agggattcgg ccgccgcgct tcatcctgcg ctcgacgatc
    19801 gcgcagatgc agcagttcac cggcacgccg gctgggcaga atccgctcgt ggccacgttc
    19861 gtggatcgca tgaacggcgt gcagtcgttg actgcctccg tgcggcagca gctgcgtgac
    19921 gaggcgacga agatcgtcga gagcgaggtc tatccggcat ggcgtaaagc cgtgtcgctg
    19981 ctcgaagccc agctccagtc ggccaccgac gacgccggcc tgtggagata caaggacggc
    20041 gcggccgtct acgcgcaccg gttgaagcag ttcacgacga cgaacatgac cgccgccgag
    20101 atccacgagt tggggctgcg cgaggtgaag c
//
LOCUS       DBNGSH010000519         7552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_49894, whole genome shotgun sequence.
ACCESSION   DBNGSH010000519 DBNGSH010000000
VERSION     DBNGSH010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_49894"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="AC16CY_11930"
     CDS             complement(<1..659)
                     /locus_tag="AC16CY_11930"
                     /inference="COORDINATES: protein motif:HMM:NF019255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HIR3354546.1"
                     /translation="MRTGIAAGVVLLCLAFAVNLRSQEQAPAPSTPRTVAPSTAAPST
                     PQRALINQYCVACHNDKLKSGNLTLSALNLESVGESAEVAEKVIRKLRGGLMPPAGAK
                     RPDNRAAAEFVSWLETGIDSGVTESRPGRVPLRRLNRREYGNAVRDLLGLNINPAAWL
                     PDDNVKGNFDNNAAALQVSPNFVDQYIHAARAVALEAIGNPRAPAETVTYGDVANMVI
                     SL"
     gene            822..2162
                     /locus_tag="AC16CY_11935"
     CDS             822..2162
                     /locus_tag="AC16CY_11935"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3354547.1"
                     /translation="MNRSLWRLAAIALVAVTVPLAGQQEVPFRGDTPIAPQGLKVPPL
                     PEAPVRYETAEGQNIRVSVYARGLRQPWSLASVSDDTMLVSERGGGGAIRVVRNGVVD
                     PQPVAGAPMVRGQGGIDLALHPNFDTNHYVYISYSKPVDTPAGRGAQPGAAPTQPGAA
                     PAQPGAAPAQPGAAAAGRGGRGGAGATTLAVARAVWDGKALTGLTDVFVGEGGGGGPI
                     TFGGDGMLYISSGGGGGGGRGGQPATQNPNTIGGKILRLTDTGAVPPDNPFVGRTDAR
                     PEVFTMGHRNTLRMAKHPGTGAIWQLEMGPNGGDEINILVPGANYGWPIVSLGRTYAG
                     PWQGPFSKEGFRDAVVLWVPSIASSSLTFYTGDKLPKWKGDVFVSGMRYGEIPGTGRL
                     DRILINKNLEELRRETLLGDLRRRIRDVKQGRDGLLYLLTDEADAVMLRIEPAE"
     gene            2249..4045
                     /locus_tag="AC16CY_11940"
     CDS             2249..4045
                     /locus_tag="AC16CY_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354548.1"
                     /translation="MNFGRRIHWMQVALVAALAIGVPMAAAAQAPQAPAGGAGAQGRG
                     GAGGGGGRGGGAPYTPAAGAKDLRAVLFNWMWHQGMLKGADERDMVATLEYQAKGGTI
                     QVDGQPCTISRLRESTNYQTFSQRVDYTCTRPNKQAVSNIEVVSWHYAWNEDTRGAEI
                     GGHKGKATATPAAVQERLIRIWANPQGAAKSALAGAMTEWRLGANPSTVIPDGVMKAG
                     NTTLSFDAAGKAVFTFPIPGVAGATGTATLDAKYLVEKVVVTHGGNTTEFTYSDYQDW
                     NNPLHKIEVFYAGRLVERKNGAVVRDITTRETETGNVYVVVPVPPSVQKAMNVAEKAP
                     TIVWAKNEPAANRTAPTPRLGKVPDLTGVWQFNDWIGNYGSRRCGPSQVACLRQTNQT
                     EDFALYSPSRFGQLTRPLYKVEHWDKVIELDMWTNKYDPVMTCQPMGLPRQGPPRRIF
                     QTENDITFLYGQFPDAGGGFPEFRVIPIDGREHGPDARYANRYFGDTVGRWEGDTLVL
                     DSIGFVDTTWIGRGGFFHSDKMHIVEKLRREGDVILYDLTLEDPEVLVEPLVFPTRAL
                     RRQTGTGASLIPERGNCETDFETEAAASQIRH"
     gene            4116..4517
                     /locus_tag="AC16CY_11945"
     CDS             4116..4517
                     /locus_tag="AC16CY_11945"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354549.1"
                     /translation="MTKWTLRLAVLSAFVLASASLVQAHHSLAGVYALGKEAKVTGAF
                     KAFRLVNPHSTMKIDVKNKDGSVTEWTFLGGSVGQIARLGLKEGPNALKPGDEITVTI
                     VPAIDGKSPVGLLSAITYADGHTVRFRSPDE"
     gene            4605..4940
                     /locus_tag="AC16CY_11950"
     CDS             4605..4940
                     /locus_tag="AC16CY_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3354550.1"
                     /translation="MPQRRNDVLQGTLALLVLKTLASTSRMHGYAITTHIQQISHDLL
                     RVEEGSLYPALHRMEQQGWLRSEWGLTDTNREARFYAITARGRRQLAAEEETWEKLTE
                     GVARVLRYA"
     gene            4948..7536
                     /locus_tag="AC16CY_11955"
     CDS             4948..7536
                     /locus_tag="AC16CY_11955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3354551.1"
                     /translation="MMSWLRRLSNTFRTRRLSSDIDREIAFHVAERAAHLLRQGVSED
                     EARRRARLQFGNPLAQRERARDVDVAEWLDALLRSVRHACRGLARTPGFTATVVLTLA
                     VGIGANTAVFSVIDAVLLRELPFPDPDRLVRLTQQTEAQGETSETNTAAVRLDEWSRL
                     SSTFVAITHFVWEDVSDTTGETPQSVRRATVGPGFLEVVGVPPALGRGFTDAEHRLGG
                     PDVALISDHYWRNRFGADPQVLGRAIRMANRPYTIVGVLPPGFTFPADVDWWVPQYLD
                     APWTRSRQFRYEGVGRLERGVSLEQAMADLERVQAQLARQFPETDRDLRPRVTPLKDV
                     YVRDVRASLWLLFGSVSLLLLIACTNIAALLLTRGAQRQQELAIRQALGSSRRTLALH
                     LLTESTVLAVAGAAAGLVLAVAMAAGLKALAPTFPRLDTVTLSGPVLIYLAVVTTAVA
                     VLSGLVPAFRITKLHTLRTGTHARATPRQPLHWWLVGVQVALSVTLLAGAGLLVRSLD
                     RLSRVDAGFDAAGVVTFRITGSFGEERDYTRIVQRINRTLDELAALPQIEAAATTLAL
                     PGLPGGVASEVDVLELGDDGKARSAELRVVSPGYFDTMQIPILEGELCRRPEDARGVT
                     EVMVNRAFAGRYIRGRAAVGLHLAATSPDRIAGVVGDAREIGIDRDPVPTVYQCFSAG
                     TPIPWFLARSAARRGVPIEDIRRTLRNVDPQRSVYDVMPLDERIGGVYAPTRVRTILL
                     SLFAITALLLACLGVYGALSYVVSLRQREAGLRLALGASRLGIIRQFCSHALRVIGAA
                     CVFGLLLSAAFTRALSGMLFGVSPLDPLTLSGVVALVLAVASIAALLPSARAAFAEPA
                     QVLRGE"
BASE COUNT         1248 a         2507 c         2569 g         1228 t
ORIGIN      
        1 agcgagatca ccatgttggc gacgtcgccg taggtcaccg tctcggcggg agccctgggg
       61 ttgccgatcg cctcgagcgc aacggcgcgc gcggcgtgga tgtactggtc aacgaaattg
      121 ggcgagacct gcagggcggc agcgttgttg tcgaagttac ccttgacgtt gtcgtcgggc
      181 agccaggctg cggggttgat gttgagcccg agcagatcgc gaacggcgtt cccgtactcc
      241 cggcggttga ggcggcgcag gggcacgcgt cccggcctgc tctcggtcac gccgctgtcg
      301 atcccggtct cgagccacga gacgaactcg gcggccgcgc ggttgtcggg ccgcttggcg
      361 ccggccggcg gcatgaggcc gccgcgcagc ttccggatca ctttttcggc gacctcggcg
      421 ctctccccga ccgactcgag attcaaggct gacaacgtca ggttgcccga cttgagcttg
      481 tcgttgtggc aggcgacgca atactgattg atcagtgcgc gctgcggggt cgagggcgcg
      541 gcggtcgaag gtgcaacggt gcgaggagtc gaaggcgcgg gcgcctgttc ctgcgagcgc
      601 agattgactg cgaacgcaag acacaacaac accaccccgg ccgcgattcc ggttctcatt
      661 cctgatccta attgtcccac ccagagggct cctgcaaagg atagactcgc ggacgcccaa
      721 gatcaatgca taatcatgtg tccgttgggg ttatcccggt aaacacgggc aagtccggga
      781 ggccgtatcc cggctgttcg tcattccctg gagatcgacg tatgaatcgc tccctctggc
      841 gcctcgccgc tatcgcgctc gtcgccgtca ccgtgccgct cgcaggccag caggaggtgc
      901 cgttccgcgg cgacacaccc atcgcgccgc agggtctcaa ggtgcccccg ctgcccgagg
      961 cacccgtccg ctacgaaacg gcggaggggc agaacatccg cgtctcggta tacgcccgcg
     1021 gcttgcgcca accgtggagc ctcgcgtcgg tctccgacga cacgatgctc gtctccgaac
     1081 gcgggggcgg gggcgcgatt cgagtcgtgc gcaacggcgt tgtcgatcca caaccggtcg
     1141 ccggcgcccc gatggtgcgc gggcaggggg ggattgatct ggcgctgcac ccgaacttcg
     1201 acacgaacca ctacgtgtat atcagctatt cgaagccagt cgacacgccg gccggccgcg
     1261 gcgcgcaacc aggcgctgct cccacgcaac caggcgctgc tccagcgcaa ccaggcgctg
     1321 ctccagcgca gccaggcgcc gccgcagcag gacggggtgg tcgcggcggc gccggcgcca
     1381 cgacgctggc cgtcgcgcga gctgtgtggg acggcaaggc actcactggt ctcaccgacg
     1441 tcttcgtcgg cgagggtggc ggcggtggtc cgatcacctt cggcggcgac ggcatgctgt
     1501 acatctcttc cgggggcgga gggggaggcg gccggggcgg ccagcccgcg acgcagaatc
     1561 ccaacaccat cggcggcaaa atcctgcggc tcaccgatac cggcgccgtg ccgcccgaca
     1621 atccgttcgt cgggcgcacg gacgcgaggc ccgaagtctt caccatgggc catcgcaaca
     1681 cgctgcgcat ggcgaagcat cctggcacgg gcgccatctg gcagctcgag atggggccga
     1741 acggaggaga cgagatcaac atcctcgtgc caggggccaa ctatggctgg ccgatcgtca
     1801 gtctcgggcg cacctacgcc ggcccgtggc aaggaccgtt ctcgaaggaa ggcttccgcg
     1861 atgcggtcgt cttgtgggtg ccctcgatcg catcgtccag cctcacgttc tacacaggtg
     1921 acaagctgcc caagtggaag ggtgacgtct tcgtcagcgg catgcgctac ggcgagattc
     1981 ccggcacggg acggctcgac cgcatcctga tcaacaagaa cctggaggaa ctgcgccggg
     2041 aaacgctgct cggcgacctg cgacggcgga ttcgcgacgt gaagcagggg cgggacggcc
     2101 tgctctacct gctcaccgac gaggcagacg ccgtgatgct gcgcatcgag ccggcggagt
     2161 gattcgcctt gaccttgtcc ctcggggggg ccatgctcac ccgaaatatt cggccgggcg
     2221 ccactgtata gatacgggga ggaccatcat gaactttggt cgacggattc attggatgca
     2281 ggtcgcgctc gtagccgccc tggcgatcgg cgtgccgatg gcagccgccg cgcaggctcc
     2341 acaggcgcca gccggcggcg ctggtgcgca gggtcgcggc ggcgccggcg gcggtggcgg
     2401 tcgcggcggc ggcgcaccct acacgcccgc cgcaggcgcg aaggatctgc gggccgtgct
     2461 cttcaactgg atgtggcacc agggcatgtt gaagggcgcc gacgagcgcg acatggtcgc
     2521 gacgctcgag taccaggcca agggcggcac cattcaggtg gacggccagc cgtgcacgat
     2581 ctcgaggctt cgcgagagca cgaactacca gacgttcagt cagcgcgttg actacacctg
     2641 cacgcgtccg aacaagcagg cagtgtcgaa catcgaagtg gtgagctggc actacgcgtg
     2701 gaacgaggac acgcgggggg ccgagatcgg cgggcacaag ggcaaggcca ctgcgacgcc
     2761 ggctgccgtg caggagcggc tgattcgcat ctgggccaat ccgcagggcg cggcgaagtc
     2821 ggcgctggcg ggggcgatga ccgagtggag gcttggcgcc aatccgagca ccgtcatccc
     2881 ggacggcgtg atgaaggccg gcaacacgac cctctccttt gacgccgcgg gcaaggccgt
     2941 cttcaccttc ccgattcccg gcgttgccgg cgccaccggc accgcgacgc tcgacgcgaa
     3001 gtatctggtc gagaaagtcg tcgtcacaca cggagggaac accaccgagt tcacctacag
     3061 cgactatcag gactggaaca acccgctgca caagatcgag gtgttctacg ccggccgatt
     3121 ggtcgagcgg aagaacggcg ccgtggttcg cgacatcacg acgcgggaga ccgagacggg
     3181 caatgtgtat gtggtcgtgc ccgtgccgcc cagcgtgcag aaggcgatga acgtcgccga
     3241 gaaagctccc acgatcgtgt gggcgaagaa cgagccggcc gccaacagga cggcgccgac
     3301 gccgcgtctc ggcaaggttc cggacctgac cggcgtctgg cagttcaacg actggattgg
     3361 caactacggc agccggcgat gcggcccctc gcaggtggct tgcctgcgac agaccaacca
     3421 gacggaggac ttcgcgctct actcgccgtc gcgcttcggc cagttgactc gcccgctgta
     3481 caaggtggag cactgggaca aggtcatcga gctcgacatg tggacgaaca agtacgatcc
     3541 cgtgatgacg tgccagccga tggggcttcc gcgtcagggc ccgccgcggc gcatcttcca
     3601 gacggaaaac gacatcacgt tcctctacgg acagtttccc gatgcgggtg gcggcttccc
     3661 ggagttccgc gtgatcccga ttgacggccg ggagcacgga ccagacgcca ggtacgcgaa
     3721 caggtacttc ggcgacaccg tcgggcgctg ggagggcgac acgctcgtgc tcgactccat
     3781 cggcttcgtg gacacgacct ggatcgggcg tggagggttc ttccattccg acaagatgca
     3841 catcgtggag aaactcaggc gcgagggcga tgtcatcctc tacgatctga cgctcgaaga
     3901 tccggaggtg ctcgtggagc ctctggtgtt cccgacacgg gcgctgcggc ggcagacggg
     3961 cacgggcgcg agcctcatcc cggaacgcgg caactgcgag acggacttcg aaaccgaagc
     4021 ggccgcatca cagatccgac attaagcgta gtgtccggct gcagccggat cggaggtcgt
     4081 gtccggccct ggccggacct gttggagaga gacacatgac caaatggact ctgcgactcg
     4141 ccgtcttgtc cgcgttcgtc ctggccagcg cctcgctggt gcaggcgcat cactccctgg
     4201 ccggcgtcta cgcgctcggc aaggaagcga aggtcaccgg ggcgttcaag gcgttccggc
     4261 tcgtgaatcc gcactcgacg atgaagatcg acgtcaagaa caaggacggc tcggtcacgg
     4321 agtggacgtt cctcggcggc agcgtcgggc agattgcgag gctcggactg aaggaggggc
     4381 ccaacgccct caagcccggg gacgaaatca cggtcacgat cgtgccggcc atcgacggca
     4441 agagcccggt cgggctgctg tcggcgatta cctacgccga cgggcacacg gtccgctttc
     4501 gctcgcccga cgagtaagcc accctcgtca cgctcgcggc accgtcgccg acggcgccgg
     4561 cttgacacat acataggata ctaggtatat acacctagta tcctatgcct cagcgcagga
     4621 acgacgtcct ccagggcact ctcgccctcc tcgtgctcaa gacgctcgcc tcgaccagcc
     4681 ggatgcacgg ctacgcgatt acgacgcaca tccagcagat ctcccacgat ctcctccgcg
     4741 tcgaggaagg ctcgctctat ccggcgctcc accggatgga gcagcagggc tggctgcgat
     4801 cggagtgggg cctgacggac acgaaccggg aggcgcgctt ctatgcgatc accgcccggg
     4861 ggcgcaggca gctcgccgcc gaagaggaga cctgggagaa actcaccgaa ggcgtcgctc
     4921 gcgtgcttcg atacgcgtga cggagccatg atgtcctggc tgcggcgcct gtcgaacacg
     4981 tttcggaccc gcagactctc gagcgacatc gatcgcgaga tcgccttcca cgtggccgaa
     5041 cgcgcggcgc acctgttgcg gcaaggtgtc agcgaggacg aggcgcggcg gcgcgcgcgt
     5101 ctccagttcg gcaacccgct cgcgcaacgg gaacgggccc gcgacgtcga cgtcgccgag
     5161 tggctcgacg cgctgctccg cagcgtccgc catgcctgcc gcgggctggc tcgcacgccc
     5221 gggttcacgg cgaccgtcgt cctgaccctg gccgtcggta tcggggcgaa taccgccgtc
     5281 ttctccgtca tcgacgccgt cctgctgcgc gagcttccct ttccggatcc cgatcgcctc
     5341 gtacggctca cgcaacagac cgaagcccag ggcgaaacca gcgaaaccaa cacggcggcg
     5401 gttcgcctcg acgaatggag ccgcctcagt tcgacgttcg tcgccattac gcacttcgtc
     5461 tgggaagatg tctcggatac gaccggggag actccgcaat cggtccggcg cgcgaccgtg
     5521 gggccggggt ttctcgaggt cgtcggcgtt ccgccggcgc tcgggcgcgg attcaccgac
     5581 gccgagcatc gcctcggggg accggacgtc gcgctgatca gcgaccatta ctggcggaat
     5641 cggttcggcg ccgatcccca ggtccttgga cgggcgatcc gcatggcgaa ccgtccctac
     5701 acgatcgtcg gcgtgctgcc tcccggattc acgtttcccg ccgacgtgga ctggtgggtg
     5761 ccacagtatt tggatgcgcc gtggacccgg tcacggcagt tcaggtatga gggcgtggga
     5821 cgactcgagc gcggcgtgtc gctggaacag gcgatggccg acctcgagcg cgtgcaggcg
     5881 caactcgcgc gacagttccc ggagacggac cgcgaccttc gaccgcgcgt gaccccgctg
     5941 aaggacgtct acgtcagaga tgtgcgcgcc tcgctgtggc tgctgtttgg atcggtttcg
     6001 ctgctcctgc tgatcgcgtg cacgaacatc gccgcgctgc tgctcacgcg cggtgcgcag
     6061 cgacagcagg agctggccat tcgtcaggcg ctcgggagct cgcggcgaac cctcgcgctg
     6121 cacctgctga cggagtcgac cgtgctggcc gtggcgggcg ccgccgccgg cctggtactg
     6181 gccgtggcga tggcggcagg gctcaaggcg ctggccccga cgttcccgcg cctcgacacc
     6241 gtgacgctga gcgggccggt cttgatatac ctggccgtcg tcacgaccgc cgtcgcggtg
     6301 ctctctggcc tggtgcccgc ctttcggatc acgaagctgc acacgcttcg aaccggcacg
     6361 cacgcgcggg ccacgcctcg tcaaccgctt cactggtggc tcgtgggcgt gcaggtcgca
     6421 ctctccgtca cgctgctggc gggcgcgggg cttctcgttc gaagcctcga ccggctgtcg
     6481 cgcgtcgatg ccggcttcga cgccgcaggc gtggtgacgt tcaggatcac cggcagcttc
     6541 ggcgaggagc gcgactacac gcgcatcgtc cagcgcatca atcgcactct ggacgagttg
     6601 gctgcgctgc cccagatcga agcagccgcg acgacactcg ccctcccggg cctgccgggt
     6661 ggtgtcgcct ccgaagtcga cgtgctcgaa cttggtgacg acggcaaagc aagaagtgcc
     6721 gaattacgcg tcgtgtcgcc cggctacttc gacacgatgc agatcccgat tctggagggc
     6781 gagctctgtc gccggccgga ggacgcgcgg ggcgtcaccg aggtcatggt gaaccgtgcg
     6841 ttcgccgggc ggtacattcg cggtcgagcg gccgtgggcc tgcatctggc tgcaacgtcg
     6901 ccggaccgaa ttgcgggcgt cgttggcgac gcgcgagaga ttggcatcga tcgggacccg
     6961 gtgccgaccg tttaccagtg cttcagtgca gggactccga ttccatggtt tctcgcccgc
     7021 tctgcagccc gtcgaggcgt ccccatcgaa gacatccgcc gtacgctccg gaacgtcgac
     7081 ccgcagcggt cggtctacga cgtcatgccc ctcgacgagc ggattggcgg cgtctatgcg
     7141 ccgacccggg tccggacgat cctcctgtcc ctgttcgcga tcacggcgct gctgctcgcg
     7201 tgcctcggcg tctacggcgc gctgagctac gtcgtcagcc tgagacagcg agaagcgggc
     7261 ttgcggctcg cgctgggagc gtcgcgcctg ggcatcatca ggcagttctg cagccacgcc
     7321 ctgcgtgtga tcggcgccgc ctgcgtattt gggttgctgc tgtcggcggc gttcacgcgc
     7381 gcgctctcgg gaatgctgtt cggagtgtcg ccactcgacc cgctgacgct gtccggcgtc
     7441 gtcgccctcg tactggccgt cgcttccatc gcagcgctgc tgccgtccgc gcgggcggca
     7501 ttcgcagaac cggcgcaggt tcttcgcggt gaatagctcc gtctgacagt aa
//
LOCUS       DBNGSH010000520         3209 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1917028, whole genome shotgun sequence.
ACCESSION   DBNGSH010000520 DBNGSH010000000
VERSION     DBNGSH010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1917028"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="AC16CY_11960"
     CDS             <1..309
                     /locus_tag="AC16CY_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR3354552.1"
                     /translation="EGAARKIQSLMAKQGIHDGGLRVGVKGGGCSGLSYTFAWEKEPR
                     LGDEVFEQHGAKIFVDRKSYLFLKGTTLDYDTALLTKGFAFSNPNAKQTCGCGSSFGA
                     "
     gene            340..1065
                     /gene="hscB"
                     /locus_tag="AC16CY_11965"
     CDS             340..1065
                     /gene="hscB"
                     /locus_tag="AC16CY_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HIR3354553.1"
                     /translation="MMSPEKIPVTAVSTFECRNCGAGAPVDEHFCPSCSRILALGRHG
                     DYFSFLGIARRLTIDQADLERRFRDLSRRFHPDYFYNAPPAERLASLERSSYLNDAYR
                     ALRSPMSRIEHLLAIEGLPPAKSENGTAKVPPALLEEVFTLNEELDDIRQSRESGADP
                     SALRRRLESARRPIEEKREEHEREVESLCAQWDRQLEAGGDADKRATLEALRERLLER
                     NYINNLLAAIDRETASLDVTRAG"
     gene            1134..2981
                     /gene="hscA"
                     /locus_tag="AC16CY_11970"
     CDS             1134..2981
                     /gene="hscA"
                     /locus_tag="AC16CY_11970"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HIR3354554.1"
                     /translation="MGKVVGIDLGTTNSLVAYVRDGVPEVIRDASGDALVPSVVSLGE
                     DGKTFYAGREAQRRLLSAPSRTVYSVKRFMGRGIDDVHEETGLLPFAVVGEPGGVVRI
                     GLGGREFAPPEISSFVLRELKQRAEEHFRDRGEFDFEVDRAVITVPAYFNDAQRTATR
                     DAGRLAGLEVLRIVNEPTAASLAYGLDRRDRGIIAVYDLGGGTFDISILKVEDGVFQV
                     LATNGDTHLGGDDVDRLLVELVLREAGGPRSAESIQAIRKAVIQAKWDLSELDEAEIR
                     VEPSPEFASGFRRRVSRAEFEQMIRPLVDRTLQPVRQAMADAALEPSDVDEVILVGGS
                     TRIPLVRRLVAELFGRTPHSELNPDEVVALGAAVQADILISGRREMLLLDVTPLSLGI
                     ETMGGVVSKIILRNSTIPATGREMFTTGVDNQTSVDIHVLQGERELAADCRSLARFRL
                     RGIPPMPAGLPRIEVRFQIDANGILSVGASELRTDIAQTIEVKPSYGLTDEEVERMLI
                     ESFEHAQADFDARLLIEARNEAETVIHATEKALRPGFAEIAAAELEPGERQRIESALA
                     DLKQVMAGSDREVIQDRTHALNHATRHLAELMMNRSVREALAGRNVKEM"
     gene            3001..>3209
                     /locus_tag="AC16CY_11975"
     CDS             3001..>3209
                     /locus_tag="AC16CY_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3354555.1"
                     /translation="MPKVTFIKDGKETTVEFEHGKLEYQHHGLPESFLDVAMNFGIPL
                     EHACGGSCACTTCHLIIRKGKENLS"
BASE COUNT          572 a         1037 c         1099 g          501 t
ORIGIN      
        1 gaaggcgccg cgcggaagat tcagtcgctg atggccaagc agggcatcca cgacggcggt
       61 cttcgtgtgg gcgtgaaggg tggaggctgc tccggcctga gctacacgtt cgcctgggag
      121 aaagagcccc ggctgggcga cgaggtgttc gagcagcacg gcgcgaagat cttcgtggac
      181 cggaagagtt acctgtttct caaagggacg acgctcgact acgacaccgc gctcctgacg
      241 aaaggcttcg cgttcagcaa tccgaacgcg aagcagacgt gcggctgcgg cagctcgttt
      301 ggagcgtaga tggagtaggc ggcggcccgc tgcctgctga tgatgagtcc agaaaagatt
      361 cccgtgacgg ccgtgtcgac gtttgaatgc cgcaactgcg gcgcgggcgc tcccgtggac
      421 gagcacttct gcccgagctg cagccggatc ctcgcgctcg gccgccatgg cgactatttc
      481 agctttctgg gaatcgcgag gcggctgacc atcgaccagg cggatctcga gcgccgcttc
      541 cgcgatctga gccgcagatt tcacccggac tacttctaca acgcgccgcc ggcagagcgt
      601 ctggcgagtc tcgagcgctc ctcgtacttg aacgacgcct accgcgcgct tcgcagcccg
      661 atgtcgcgca tcgagcacct gctggccatc gagggtctgc cgccggcgaa gtcagagaat
      721 ggcacggcga aagttccgcc cgccctgctc gaggaagtgt tcacgctgaa cgaggaactg
      781 gacgacatcc ggcaatcgcg cgagtcgggt gccgatccgt ctgcgcttcg ccgcaggctc
      841 gaatcggctc gcagaccgat cgaggagaag cgcgaggaac acgagcggga ggtcgagtcg
      901 ctctgcgcgc agtgggatcg acagctcgaa gcgggcggcg acgccgacaa gcgggcgacg
      961 ctggaggcgc tgcgggagcg cctgctcgag cgcaactaca tcaacaacct gctcgccgcc
     1021 atcgatcgcg agacggccag cttggacgtg acacgcgcgg gctgaagccc gcgctctacg
     1081 acggcgagaa ggacgagagg gtagagcggg ggcgtcagcc cccgcggacg agaatgggca
     1141 aagtcgttgg catcgacctc gggacgacga acagtctggt tgcctacgtg cgcgacggcg
     1201 tgcccgaagt catacgggac gcgtcgggcg acgcgctcgt cccgtcggtc gtgtctcttg
     1261 gcgaagacgg caagaccttc tacgccgggc gggaagccca gcgccggctg ctctcggctc
     1321 cctcgcggac cgtgtattcg gtcaagcggt tcatgggccg cggcatcgac gacgtgcacg
     1381 aggaaaccgg gctgctgccg ttcgcggtgg tcggcgagcc tggcggcgtc gtgcggatcg
     1441 ggctcggcgg ccgcgagttc gcgccgcccg agatttcctc cttcgtgctc cgcgagctga
     1501 agcagcgggc cgaagagcac ttccgcgatc gaggcgagtt cgatttcgag gtcgatcgcg
     1561 ccgtcatcac ggtgcctgcc tacttcaacg acgcgcagcg gacagccacg cgcgacgccg
     1621 gaaggctggc cggcctcgaa gtgctgcgga tcgtgaacga gccgaccgcc gcctcgctgg
     1681 cctacggcct cgaccgccgc gaccgcggca tcatcgcggt ctacgacctc ggcggcggca
     1741 cgttcgacat ctcgatcctg aaggtcgagg acggcgtctt ccaggtgctg gccaccaacg
     1801 gcgacacgca cctcggcggc gacgacgtcg atcgcctgct cgtggagctc gtgcttcgcg
     1861 aggcgggcgg gccgcgatcc gccgaatcga tccaggcgat ccgcaaggcc gtgatccagg
     1921 cgaagtggga tctctcggag ctggacgagg cggagatccg agtggagccg tcccccgagt
     1981 tcgcgagcgg cttccgacga cgagtgtcgc gcgcggagtt cgagcagatg attcgtccgc
     2041 tcgtagaccg gacgctgcag cccgtccgcc aggcgatggc ggatgccgct ctcgagcctt
     2101 ccgacgttga cgaagtcatc ctcgtcggcg gctcgacgcg aattccgctc gtgcgcaggc
     2161 tcgtggcgga gctcttcggc cgtacgcctc acagcgagct caatcccgac gaggtcgtcg
     2221 cgctcggcgc cgccgtgcag gcggacatcc tgatcagcgg ccgccgcgag atgctcctcc
     2281 tcgacgtcac tcccttgagc ctcggcatcg agacgatggg cggggtcgtg tcgaagatca
     2341 tccttcgcaa ttcgacgatt ccggcgaccg gccgcgagat gttcacgacg ggcgtggaca
     2401 accagacctc agtggacatc cacgtcctcc agggcgaacg ggaactggcg gccgactgcc
     2461 ggtcgctcgc ccgtttcagg ctgcgcggca ttccgcccat gccggcgggc cttccgcgca
     2521 tcgaggttcg cttccagatc gacgcaaatg gaatcctcag cgtcggcgcc agcgagctcc
     2581 gcaccgatat tgcgcagacc atcgaggtga agccgtccta cggcctgacc gacgaggagg
     2641 tcgagcgcat gctcatcgag tcgttcgagc acgcgcaggc cgacttcgac gcgcggctgc
     2701 tcatcgaggc caggaacgag gccgagacgg tcattcatgc caccgagaag gccctgcgcc
     2761 caggctttgc cgagatcgcc gccgcagagc tcgagcccgg agagcgccag cgcatcgaga
     2821 gcgcgctggc cgacctgaag caggtcatgg ctggatcgga ccgggaggtg attcaggaca
     2881 ggacgcacgc gctcaatcac gcgacgaggc acctggccga gctgatgatg aatcgcagcg
     2941 tgcgcgaggc gctcgctggc cgcaacgtga aagagatgtg aatcgccggt tgacgcacag
     3001 atgcccaagg tcactttcat caaggacggc aaggagacga cggtcgaatt cgagcacggg
     3061 aagctcgagt accagcacca tggcctgccg gagtcgtttc tcgacgtcgc catgaacttc
     3121 ggcattccgc tggagcacgc ctgtggcgga agctgcgcct gcacgacgtg tcacctcatc
     3181 attcgcaagg gcaaggagaa cctgagcga
//
LOCUS       DBNGSH010000521         1775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3106466, whole genome shotgun sequence.
ACCESSION   DBNGSH010000521 DBNGSH010000000
VERSION     DBNGSH010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3106466"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1242
                     /locus_tag="AC16CY_11980"
     CDS             160..1242
                     /locus_tag="AC16CY_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3354556.1"
                     /translation="MRSAVAILCGAILAGCSGSDVPAQPPAAPRAATAPSPEAAARTR
                     TIRWSGTLEAVRSTPVTVPDVTGQTFRMTLTRLVRNGATVAKGDIVAEFDPIEQLDQA
                     RDRANRFEDLSYQVRQRQADNAANAERRRSEREQAEADLAKAKLEVSKAPVLSDIDAK
                     QNAIRAEKAQARLESLRRTQPQEERSDHATLRVLELQRDRQKAALDRIRANLERLSVR
                     APLGGLVAHSTFYNNGAMVRPQEGDQMTRNNAIMRIFDPSEMLVRVSVAEPDGALLDE
                     GLEAMVYVDAYPDLSLPALFVSASPVAASTGLGRGTKSFMAVFRLKGSDPRLMPDLSA
                     AVVFEERETPAKPAQPDLASSTRAAR"
     gene            1239..>1775
                     /locus_tag="AC16CY_11985"
     CDS             1239..>1775
                     /locus_tag="AC16CY_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354557.1"
                     /translation="MTGFRGLVAIVMTASVIAAIWIGTRQFVQAERSELPIAPVRSGE
                     FVAVIRTRGQIQAQRSVPIYAPLVQDLRIAWMAPASERIEQGAPLIRFDSSTVERDLI
                     QRRATAERARAALDQAIADGKAAAEHDQRDLQDARLAVEIAQLDTKDSEFVARLDAER
                     GRIDLRVAEQKLRQLEAEV"
BASE COUNT          324 a          613 c          582 g          256 t
ORIGIN      
        1 ctggatatgt tcgagcgcga tgtttacggg catatcgttc ctcccgtccc ccgaagcccc
       61 tcgagactgt cctgttaaga tccgccagtc agcccgaatg ggggccgacg gcgtctaagc
      121 aaacgacccg gccgccacgt atttggcatg aggaccagca tgcgctctgc cgtcgcgatt
      181 ctgtgtggcg ccatcctggc tggttgctcg ggatcggacg tccctgccca gccccccgct
      241 gcgcccaggg cggcgacggc gccgtccccc gaggccgccg cgcgaacacg gaccatccgg
      301 tggagcggca cgctcgaagc agtcaggtcc acaccggtca ccgtaccgga tgtgacaggc
      361 caaacgttca ggatgacgct cacacgcctc gtcaggaacg gcgcaacggt cgccaagggg
      421 gacatcgtcg ccgagttcga tcctatcgaa caactcgatc aggcgcgcga tcgagcgaac
      481 cgcttcgagg atctctccta tcaggtcagg cagcggcagg ccgacaacgc ggccaacgcc
      541 gaacggcgcc gctccgaacg cgagcaggcc gaggcagacc tcgcgaaagc gaagctcgag
      601 gtgagcaagg ccccggtcct gagcgacatc gacgccaagc agaacgccat ccgggccgag
      661 aaggcccagg cgcgcctcga gagcctgcgg cgcacgcagc cgcaagagga gcgctccgac
      721 catgcgacgc tccgggtcct cgaactgcag cgcgaccggc agaaggcggc gctcgatcgg
      781 atacgcgcca atctggaaag gctgagtgtc agggcgccgc tcggtggatt ggtcgcgcac
      841 tcgaccttct acaacaacgg cgccatggtt cgcccgcagg agggcgatca gatgacgcgc
      901 aacaacgcga tcatgagaat cttcgatccc agcgagatgc tcgtgcgcgt aagcgtcgcc
      961 gagcccgacg gagccctgct ggacgagggc ctcgaagcca tggtgtacgt ggacgcctat
     1021 cccgacctgt cactgccggc gctcttcgtg agcgcgagcc ccgtggccgc gtccaccggg
     1081 ctgggccgcg ggaccaagag tttcatggct gtctttcgac tcaagggctc ggatcctcga
     1141 ctcatgccgg atttgtcggc cgccgtcgtg ttcgaggaac gcgagacgcc ggcaaagccc
     1201 gctcaacccg acctcgcttc gtccacgcga gccgcgcgat gacgggattc cgcggcctgg
     1261 tcgcgatcgt catgacggct tccgtcatcg cggccatctg gatcggcacg cgtcagttcg
     1321 tccaggccga acgatccgag ctgccgattg ctccggtgcg gtcgggagag ttcgtcgccg
     1381 tcatccggac gagaggacag attcaggccc aacgatccgt gccgatctac gccccgctcg
     1441 tgcaggactt gaggatcgcg tggatggcgc ccgccagcga gcgcatcgag cagggggcac
     1501 ccctgatccg cttcgattcg agcacggttg aacgcgacct gatccagcgc cgcgcaacgg
     1561 ccgaacgcgc gcgcgccgcc ctggaccagg ccattgccga cggcaaggcc gccgccgagc
     1621 acgaccagcg agaccttcag gacgcccgac tcgccgtgga gatcgcgcag ctcgacacca
     1681 aggacagcga gttcgtggcg cgcctggatg ccgagcgcgg ccgcatcgac ctgcgcgtcg
     1741 ccgaacagaa gctgcggcag ctcgaggcgg aggtc
//
LOCUS       DBNGSH010000522         7031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1578020, whole genome shotgun sequence.
ACCESSION   DBNGSH010000522 DBNGSH010000000
VERSION     DBNGSH010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1578020"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="AC16CY_11990"
     CDS             complement(<1..910)
                     /locus_tag="AC16CY_11990"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3354558.1"
                     /translation="MGVSKMRPFGKTIALDEARAIVEGAIRPIERTEAVPLLEANGRV
                     LAQDVVSNADVPPFSRAAMDGYAVRAGDTAGATRASPRRLQRIETIFTGQVPRRRVEA
                     GQCAEVATGAPMPDGADAVVIVEDTDIDESGRVAVFVSVEPRQNIGQQGADIRKGQRV
                     LEPGAALNASRVGALAALGMTVVRVYGKPRVAILSTGNEIVDPGQTLAPGQIYDINRV
                     TLSSVVDDNGGVPVPLRTAADTIEDLMRAVDECLEQDLMVFSGGSSVGERDLILDAIA
                     ARGEVLFHGIAVKPGKPTAFGRIDGKL"
     gene            complement(998..1501)
                     /locus_tag="AC16CY_11995"
     CDS             complement(998..1501)
                     /locus_tag="AC16CY_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR3354559.1"
                     /translation="MSVGEHKARAPRAVPCFVLTVSDTRTPDTDTSGRAIRDLLAGAG
                     HSITGHDLVRDEPDHVTARVRERLSDPGTQAIITTGGTGISARDGTFEAIDRLLEKRL
                     DGFGELFRMLSFEEIGAAAMLSRATAGTVGRKAVFALPGSENAVRLAMTRLVLPELGH
                     VIQQLLK"
     gene            1612..2232
                     /locus_tag="AC16CY_12000"
     CDS             1612..2232
                     /locus_tag="AC16CY_12000"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR3354560.1"
                     /translation="MTEHNHAGQRPTAAEALARLREGNERFTRGEARFPTIQKEILAD
                     LAKGQQPWATILGCSDSRVPPELVFDAGFGELFIVRVAGNVVSPEVLGSLQYAAVHLR
                     TPLFVVLGHEGCGAVKAALAATFQGARDRSRIERLLENITPGLTGIDADSDPEEMLKA
                     GVEANVRWSMHQLLETPEAKQRQQEGLLKLVGAVYELTTGRVRFLA"
     gene            complement(2271..3413)
                     /locus_tag="AC16CY_12005"
     CDS             complement(2271..3413)
                     /locus_tag="AC16CY_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR3354561.1"
                     /translation="MGVLGTIALLLACPCAALAQEPAAAAASAERTPSRFRSPDDGWL
                     DISQFLDTRYGFLPVAQLITEPAVGFGAAGGLAFIKQPPGSMRPNITVVGGLGTENGT
                     KGAIAADLRYWLDGRLQTLAGVVFASVNLDYYGIGHDSVLAENPLRYELEPTGGVVEA
                     KIRIGRSPVWVGARYVYAQTGVRFDAPEGTPGLPETARWSAEAGITPSVTVDARDNLF
                     TPTRGTYIEAGAGLFSPTLGGDDSFQRLRLIAMQFVPLGTRFFLGVRGEAAAALGDAP
                     FYVKPFIYQRGVPAMRYLGEEMAQIETELRWQFWKRFSVVGFGGAGRTWAGSEEVDLS
                     DKVAAGGGGFRYELARKHGLHVGVDVAGGPAGGVFYIQFGSAWAKP"
     gene            3460..3699
                     /locus_tag="AC16CY_12010"
     CDS             3460..3699
                     /locus_tag="AC16CY_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354562.1"
                     /translation="MEFGSLVIVAVMVLFVAAVVKARRRRTHTGGPGPGATGFIYELL
                     SEDRRNAIAIIAEEKAEARDPETADGNLPELERPK"
     gene            complement(3705..3890)
                     /locus_tag="AC16CY_12015"
     CDS             complement(3705..3890)
                     /locus_tag="AC16CY_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354563.1"
                     /translation="MKDYTARPIDLDPAMVEYLEGAAREFSLSDIGKAVRCLINYARE
                     NPDRRRDIFDEVRCVGC"
     gene            4027..4103
                     /locus_tag="AC16CY_12020"
     tRNA            4027..4103
                     /locus_tag="AC16CY_12020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4061..4063,aa:Arg,seq:acg)
     gene            4376..5698
                     /locus_tag="AC16CY_12025"
     CDS             4376..5698
                     /locus_tag="AC16CY_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3354564.1"
                     /translation="MRCLTVAVSVCLSLGLAACRSADAAAGGAEAAAASQPCAMALAP
                     HQGTGDVDQGIARSQQAAREGANPAAALNDLGQRYIARARASQAPTYYAMAEFAALCA
                     ESKDPGNAQALLLRGHVLHQQHRFKEAEVVARAVAAKRGLYLDYGLLGDVLLELGHVE
                     EGGQAYQRMMDLKPFFHSYTRASHYRWLKGDLPGALELMHQAVRTASPRDPESVAWAF
                     SRLAAYELQAGRTAEALRDSEAALQHQPNYAAALLIRGRTLLSDGKHGEAVDALRRAA
                     LASPLPEHEWALADALRAAGDLSEARRVEERLNQTGAVNDPRTFALYLATRRERPDRA
                     VALAEQELRNRSDVFTFDALAWSLASAGRTMEAEQAMTRALAEGTKDARLFYHAGVIA
                     ASGGHVTEARRWLREARSVQQTLLPSERQDLTEQITSLTRAQNRRKAI"
     gene            5695..>7031
                     /locus_tag="AC16CY_12030"
     CDS             5695..>7031
                     /locus_tag="AC16CY_12030"
                     /inference="COORDINATES: protein motif:HMM:NF025585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HIR3354565.1"
                     /translation="MRKLTPTVAGALVAASLALAPPPAVASSHMDAPLITLDDAANTT
                     DVYAFITMYNGTKSLAVALSVYPFEEPGIGPNKYNFDDDVLYEIHVAAGNADVQAGRA
                     TYTYQFRFSTTFRNRNTILQSYLGVIQDVGDAAQNLVQRYTVTKVDRRSGQRFTLGSG
                     VVPPNNQGNATPFYNQNNNGESPARDGVATDSELDRYTSQSIGFLADGYTAFAGQRDD
                     GFYADVQAVFDLLKLRSPGSDSQGGYNVHTMSLNIPLDELGGDQQVLGVYATASRRTL
                     TILSEGRQAPIKLGSFVQVARQGNPLFNEALVAIEDKDLYSRTSPTSDATLFRKYALN
                     PELAVLLNALVFGSNVAPTTNRTDIAGIFIPDLIKVDASTEPARLAGGGPSHPTNPDD
                     AGFSRLSIFGNDVLFSPLKKGFQPGGWPNGRRFGDDVLDIAVSALISDLRGTSP"
BASE COUNT         1173 a         2419 c         2350 g         1089 t
ORIGIN      
        1 ccagcttccc gtcgatgcgg ccgaaggccg tgggcttgcc cggcttgacc gcaatcccgt
       61 ggaagagcac ctcgcccctg gccgcgatcg cgtcgaggat caggtcgcgt tcgccgacgg
      121 aactgccgcc cgagaacacc atcaggtcct gctcgagaca ttcgtcaacg gcgcgcatga
      181 ggtcttcgat ggtgtcggcc gccgtgcgca gcgggaccgg cacgccgccg ttgtcgtcga
      241 cgaccgagga gagagtgacg cgattgatgt cgtagatctg tcccggggcg agcgtctgcc
      301 cgggatcgac gatctcgttg cccgtcgaga ggatcgcgac gcgcggcttg ccgtacacgc
      361 ggacgacggt catcccgagc gccgccagcg cgccgacgcg gctcgcgttg agcgccgcgc
      421 ccggctcgag cacgcgctgt cccttccgga tgtcggcgcc ctgctggccg atgttctgac
      481 ggggctcgac cgacacgaac actgcgacgc ggcccgattc gtcgatgtcg gtgtcttcga
      541 ctatcacgac ggcgtccgcg ccatcgggca tgggcgcgcc ggtcgccacc tcggcgcact
      601 gtccggcttc gacgcgccgc cgaggtacct gccccgtgaa gatcgtctcg atgcgctgga
      661 gacggcgggg agaagcgcgc gtggcgcccg cggtgtcgcc ggctcgaacg gcgtagccgt
      721 ccatcgccgc tcgcgagaac ggaggcacgt cggcgttcga gacgacgtcc tgcgcgagga
      781 cgcgcccgtt ggcctcgagc agcgggacgg cttcagtccg ttcgatcggc cggatcgcgc
      841 cctcgacgat ggcgcgcgct tcgtccaacg cgatcgtctt gccgaacgga cgcattttcg
      901 aaacgcccaa ctcccaaact cccaactccc aacagctcga ttcacggcgt tggaagcagt
      961 tcgtacgtag tgtccggctt cagccggacc gtgacgttca tttcaacagc tgctgaatca
     1021 cgtgccccag ctccggcagc acgaggcgcg tcatcgccag ccgcacggcg ttctccgacc
     1081 ccgggagcgc gaacacggcc ttgcgtccca cggtgccggc cgtcgcgcgg ctgagcatcg
     1141 ccgcggcgcc gatctcctcg aagctgagca tgcgaaagag ctcgccaaag ccgtcgagac
     1201 gcttctcgag cagccgatcg atcgcctcga acgtgccgtc gcgcgcgctg atgccagtgc
     1261 cgccggtcgt gatgatcgcc tgtgtccccg ggtccgacag ccgctcgcgc actcgagccg
     1321 tgacgtgatc cggttcatcg cgcacgaggt cgtgcccggt gatgctgtgg ccggcgcccg
     1381 cgagcagatc gcggatggcg cggccgctcg tatccgtgtc cggcgtgcgc gtatcgctga
     1441 cggtcaacac gaagcacgga acggcacgcg gcgcgcgcgc tttatgttcg ccgacgctca
     1501 cgtcgggatt ataggcccca ggccccaggg ctcaggcctc agcagggccc agggctcagg
     1561 cctccgcagg gctcaggggt tcagtcccca gcggtcataa tggagttccc ggtgactgag
     1621 cacaatcacg ctggacagcg tcccaccgcg gcagaagcgc tggcacggct gcgcgaggga
     1681 aacgaacgat tcactcgcgg cgaagcgcgc tttccgacca tccagaagga gatcctcgcc
     1741 gacctggcga agggtcagca gccgtgggcg acgatcctcg ggtgcagcga ctcccgcgtg
     1801 ccgcctgaac tcgtcttcga tgcgggattc ggagagctgt tcatcgtgcg cgtcgccggg
     1861 aacgtcgtct cgccagaagt gctggggagc ctgcagtacg cagccgtcca tctgcgcact
     1921 ccgcttttcg tcgtgctcgg ccacgaaggg tgcggcgcgg tcaaggcggc cttggcggcg
     1981 acgttccaag gcgcccggga tcgaagccgg atcgagcgcc tcctcgagaa catcacgccc
     2041 ggtctgaccg gcatcgatgc ggattcagat ccggaggaga tgctcaaggc gggcgtcgag
     2101 gccaacgtgc gctggtcgat gcaccagcta ctggaaaccc cggaggcgaa acaacgtcag
     2161 caggaaggcc tcttgaagct ggtcggcgcc gtgtacgagc tgaccaccgg gcgcgtgcgc
     2221 ttcctggcgt gacgagcggg cggccggcgc ctgcgcccgc gtacgatgaa ctacggcttc
     2281 gcccaggcgc tgccgaactg gatgtagaag acgccgccgg ctggtccgcc ggcgacatcg
     2341 acgccgacgt gaaggccgtg cttccgcgcc agctcgtagc ggaagccgcc tccgcccgcg
     2401 gccaccttgt ccgacaggtc gacttcctct gagccagccc aggtgcgccc ggcgcccccg
     2461 aagccgacca cgctgaatcg tttccagaac tgccatcgaa gctcggtctc gatctgcgcc
     2521 atctcctcgc cgagataccg cattgcgggt acaccgcgct ggtagatgaa cggcttcacg
     2581 tagaacggcg cgtcgccgag cgccgcggcc gcctctccgc gcacgcccaa gaagaacctg
     2641 gtgcccagcg gcacgaactg catcgcaatc aggcgcagac gctggaacga atcgtcgccg
     2701 ccgagcgtcg ggctgaacag gccggccccg gcctcgatgt aggtgccgcg agtgggcgtg
     2761 aacaggttgt ctcgcgcgtc gacggtcacg ctcggcgtga tccccgcctc ggccgaccag
     2821 cgcgccgtct cgggcagtcc cggcgtgccc tccggcgcat cgaagcgaac gcccgtctgc
     2881 gcgtagacgt acctcgcgcc gacccagacg ggcgatctac caatacggat cttcgcttcg
     2941 accacgccgc ccgtgggctc gagctcgtag cgcaggggat tctcggcaag gacgctgtcg
     3001 tggccgattc cgtagtagtc caggttgacc gacgcgaaga cgacacctgc aagcgtctgc
     3061 agccggccgt cgagccagta gcgaagatcg gcggcgatcg cgcccttcgt gccgttctcc
     3121 gttccgagcc cgccaaccac ggtgatgttg ggccgcatcg aacccggagg ctgcttgatg
     3181 aacgccagcc ccccggcggc gccgaagccg acggcgggtt cggtaatcag ctgggcgacc
     3241 ggcaggaacc cgtagcgtgt gtccaggaac tggctgatgt ccagccaccc gtcgtcgggg
     3301 gatcggaacc tggagggcgt acgctccgcg ctcgccgcgg cggccgctgg ttcctgcgcc
     3361 agcgccgcac acgggcacgc cagcaggagg gcgatcgtgc ccaggacccc caatgcgcgc
     3421 gaccgtcgcc taagcgtgag cccgtgagag gatcgcctca tggagtttgg cagtctcgtc
     3481 atcgttgccg tgatggttct gttcgtcgcg gcggtggtca aggcccggcg tcggcggacc
     3541 cacacgggag gccccggccc gggcgcgacc ggcttcatct acgagctgct gagcgaagac
     3601 aggcgcaacg cgatcgcgat cattgccgag gagaaagccg aggcgcggga tcccgagacg
     3661 gccgacggca acctgcccga gctcgagcgg ccgaagtagg ctcgtcagca gccgacgcag
     3721 cgcacctcgt cgaagatgtc gcggcgtctg tcggggttct cgcgcgcgta gttgatcagg
     3781 caccgtacgg ccttaccgat gtccgagagc gagaactccc tggcggcgcc ttcaaggtat
     3841 tcgaccatgg cgggatcgag atcgatcggt cgggcggtgt agtccttcat gggcctcctc
     3901 gtggctggcc accgatgata gctgctctcg cgccgtcggg ctgcgccatg acgccgtgat
     3961 atagtcaatg attgcgcgca cttggaccga tgctcgagtg cgcaccgcga tcgattcaac
     4021 ccatccgcgc ccttagctca gctggataga gcgtctggct acgaaccagg aggtcgcacg
     4081 ttcgagtcgt gcagggcgca ccaaccttcg ctcacgctta gcaacgcgcg cgtgagctac
     4141 ggttggcaag ccacgcgaag gttgtccgcc gcagcgcacg tctcagtgac tccgcttctc
     4201 gccttcggct cgcagcgagc gggctcgagt cgtgcacggc cctttcgcgc tgaacgagct
     4261 caaacctcgc ctctgagacc gacaccatcc gattccgctc cgcgtccgta gtaatcgttg
     4321 cggagccgga aacgccacga cggcgagtcc aaaacaatcg ttcgagcgag gtcccatgag
     4381 atgcctgacg gtagcggtgt cggtgtgtct gtctctcggc cttgcggcct gccgcagcgc
     4441 tgacgcggcg gccggaggcg ctgaagccgc ggccgcgtcc cagccgtgcg cgatggccct
     4501 ggcgcctcat cagggaacgg gcgacgtcga tcagggcatc gcgcgatcgc agcaggcggc
     4561 gcgcgagggg gccaatcccg ccgcggcgct caacgatctc ggccagcggt acatcgcgcg
     4621 ggcgcgcgcg tcccaggcgc ccacctacta cgcgatggcc gaattcgcgg cgttgtgcgc
     4681 cgagtcgaaa gacccgggca acgcgcaggc gctgctgctg cggggccacg tgctgcacca
     4741 gcagcatcgc ttcaaggaag ccgaggtcgt ggcccgtgcg gtcgccgcga agcgggggct
     4801 gtatctcgac tacggtctgc tcggcgacgt gttgctcgag ctgggccacg tcgaggaggg
     4861 cggccaggcc taccagcgga tgatggacct caagcctttt ttccactcct acacgcgagc
     4921 ctcgcattac cggtggctca agggcgacct gccgggcgcg ctcgagctga tgcaccaggc
     4981 cgtcaggacg gcgagcccgc gcgatcccga atcggtggca tgggcgttct cgcggctggc
     5041 cgcgtacgag ctgcaggccg ggcgcaccgc agaggccctg cgcgactcgg aagccgccct
     5101 tcagcatcag ccgaactacg ccgccgcgct cctgattcga ggccgcacgc tgctctcgga
     5161 cggaaaacac ggcgaggcgg ttgacgcgct ccggcgcgcc gcgcttgcga gcccgctccc
     5221 cgagcacgag tgggccctgg ccgacgcgtt gcgcgcggcc ggcgatctct cggaggcgcg
     5281 aagggtcgag gagcggctca accagaccgg cgccgtcaac gatccgcgga cgttcgcgct
     5341 gtatctggcc acgcggcgcg agcggcccga tcgggccgtc gcgctcgcgg agcaggagct
     5401 gcgaaaccgg agcgacgtgt tcaccttcga cgcgctcgcc tggtcgctcg cctcggccgg
     5461 ccggacgatg gaagccgaac aggcgatgac gcgtgcactc gctgagggga cgaaggacgc
     5521 gcggctcttc tatcacgccg gcgtcatcgc cgcgtcaggc ggacacgtga cggaggcacg
     5581 gcgctggttg cgcgaggccc ggtcggttca gcagacgctg ctgccgtccg agcgacagga
     5641 cctgaccgaa cagatcacat cattgactcg ggctcagaac aggagaaagg cgatatgagg
     5701 aagctcaccc caacggttgc cggcgcgctc gtggccgcgt ccctcgcgct ggcgcctccg
     5761 ccggccgtcg cgtccagcca catggacgcg ccgctcatca cgctggacga tgccgcaaac
     5821 accaccgacg tctacgcgtt catcacgatg tacaacggca ccaagtcgct ggccgtcgca
     5881 ctgtccgtct atccattcga ggagccgggc atcgggccga acaagtacaa cttcgacgac
     5941 gacgtgctct acgagattca cgtggccgcg ggcaacgccg acgtccaggc cggccgcgcc
     6001 acctacacct atcagttccg cttctcgacg acgttcagga acaggaacac gatcctccag
     6061 tcgtacctgg gcgtcatcca ggacgtcggg gacgccgcgc agaacctggt gcagcgatat
     6121 acggtcacga aggtggaccg gcgctcgggg caacgcttca cgctcgggag cggagtcgtc
     6181 ccgccgaaca accaggggaa cgccacgccc ttctacaacc agaacaacaa cggcgagagt
     6241 cccgcgcggg acggagtggc caccgattcc gagctcgaca gatacacgag ccagtcgatc
     6301 ggattcctgg cggacggcta caccgcgttc gcgggccagc gcgacgacgg cttctacgcc
     6361 gacgttcagg ccgtgttcga cctgctgaag ctcagatcgc cgggcagtga ctcgcagggc
     6421 ggctacaacg ttcacaccat gtcgctcaac atcccgctcg acgagctcgg gggcgatcag
     6481 caggtgctcg gcgtatacgc gaccgcgagc cgcaggacgc tgacgattct gagcgagggc
     6541 cggcaggctc caatcaagct cggctcgttc gtgcaggtgg cccgtcaggg caatccgctg
     6601 ttcaacgagg cgctggtggc gatcgaagac aaggatctct acagccggac ctcgcccacc
     6661 tcggatgcga cgctcttcag gaagtacgcc ttgaacccgg agcttgcggt gctgctcaac
     6721 gcgctggtgt tcggcagcaa tgtcgcgccc acgaccaatc gcaccgacat cgccgggatc
     6781 ttcatccccg atctgatcaa ggttgacgcg tcaacggagc cggcgaggct ggccggcggc
     6841 ggaccgagcc atcccaccaa tcccgacgat gcgggctttt cgcgcctcag cattttcggc
     6901 aatgacgtcc tgttcagccc cctcaagaaa ggcttccagc cgggcggatg gccgaacggc
     6961 cgccggtttg gagacgacgt attggacatc gcggtatcgg ccctgatcag cgatctgcgc
     7021 gggacctcgc c
//
LOCUS       DBNGSH010000523         5565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_559524, whole genome shotgun sequence.
ACCESSION   DBNGSH010000523 DBNGSH010000000
VERSION     DBNGSH010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_559524"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="AC16CY_12035"
     CDS             complement(<1..535)
                     /locus_tag="AC16CY_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3354566.1"
                     /translation="MHPHLAEVLGRLDAAGGAVSAAVAGVPEGLRRTRPAPGRWSVAE
                     VLEHLALVDRSFAQRMADAIAEARVAGLGPEDRQRDPLPPAIAHRMADRGEAREAREA
                     LRPAGGLDAAAAAAELDAARDAVRQAVSPADGLALGSVSAEHRIFGSLTMYQWVELTA
                     AHEMRHADQIREIAGALG"
     gene            complement(545..1177)
                     /locus_tag="AC16CY_12040"
     CDS             complement(545..1177)
                     /locus_tag="AC16CY_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIR3354567.1"
                     /translation="MGKYVVECVGTFFLVLTIGMTVIEPGAGSLAPLAIGAALMVMVF
                     AGGHVSGAHYNPAVTLAVWLRGKCPSRDVPIYMGVQVVGGLIAAAAVGVLKGSAMAQV
                     TERSLDVAQALLAEFLFTFALAWVVLNVATAKGTSGNSFYGLAIGFTVMTGAFAVGGV
                     SGGAFNPAVAVGATAMGLAAVSNLWIYLVADFAGAAVAAVTFKTVNPQDK"
     gene            1312..2238
                     /locus_tag="AC16CY_12045"
     CDS             1312..2238
                     /locus_tag="AC16CY_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3354568.1"
                     /translation="MHVRRLVSLTLLPLLAFLTCGPTAAQSDGTQATFKSGVDLVSVA
                     ATVRDKKGRVVRSLTSKDFIVTDEGQRRTIIGMQADDGSPANLALLVDGSGSMKVGTS
                     GDASRLICHMILTRLRRDQDRAALMSFDTRLLTLREFTAEFDHIQTGLGEVAPFGSTS
                     IYDAIAGTAAAVAKGTQKRRAVVVITDGWDNASTYTPQQVAAIASTIDVPVYVFAVGD
                     LNPETVANEKTVAERRSDLFDLARATGGDHFFSGSPSIVHQAATRLLDELHHQYLIAF
                     EPATASGLRRIEVRTRNDDHNVRSRRWYAAAN"
     gene            2370..3461
                     /locus_tag="AC16CY_12050"
     CDS             2370..3461
                     /locus_tag="AC16CY_12050"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 5 family protein"
                     /protein_id="HIR3354569.1"
                     /translation="MFWHGCNYPWSTDGTSSCYGLDFGANVWGTHAGVSTRRQRIESD
                     FSRMASLGFTVARWFVFADGRSGITYDEGGRPAGPDPHLFSDVDAALEIARGANMRLN
                     LVLLDHRWMFDEVPDVIADPATGLLLETRLPHGRAHVLRVQEGRDALMRTVLEPLVRR
                     YGRKGERADLGPHVFAFEFMNEPDFVVEEWERDVSAHVRRPLPFESLAELVAGLSAIV
                     HEHTGAFTTIAAARLHNLWAWDDDQLGLDVLQVHSYPDTRHPERDADVLGLPAASLGV
                     RRPVILGEFPANGPRQHPADSARPPTELERYLEFALTAGYAGAWPWSFSGTDAYGRLP
                     EDPLRAFARRHPDLVNPRALLEPGGPAPT"
     gene            3486..4079
                     /locus_tag="AC16CY_12055"
     CDS             3486..4079
                     /locus_tag="AC16CY_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024937.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR3354570.1"
                     /translation="MDLITLGRTLGFSFAAGVNLYATVAILGLASRYGWVELPAQFQV
                     FNNDVVIGAAIVMYAVEFFADKIPYFDSVWDMIHTFIRPVGGALIAVATLGDASTPVE
                     GLVALLGGTIAASSHLTKTSTRAMANTSPEPLSNWALSLGEDVFVVGLGYLALKHPIA
                     ALVVAAVLLGAIAVFAAVIIRTVRRWFFRRQIPSPAG"
     gene            4126..5265
                     /locus_tag="AC16CY_12060"
     CDS             4126..5265
                     /locus_tag="AC16CY_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sensor domain DACNV-containing protein"
                     /protein_id="HIR3354571.1"
                     /translation="MSRHKHPAAQLVAPKVEAYFAQHLEAARQRGDEHVAPLPTADAI
                     ETMIDAAFWASLRREEGYVPKISLAFLPPDLDPYPMLLERPLALDPGVLTKVAPVVDR
                     AGIHLGVWPRNGELSVWGTTHTIPPLCFVLEVVEPGLLVVKHHRGEQSGKYVNVAVLE
                     GDQIKIVDETASSLPDCPALLTSLLGFDSPASWAGPVNVLVQLAVSMRTHRRGGTLLI
                     VPAGTDRWRESIVSPIPYAIEPPYRELADLIREGRSERRSDTWQEALRETVDAIAGLT
                     AVDGATLLTDSYELLAFGAKIARRRGAPRVDEITLTEPIEGGTASVISPVQLGGTRHL
                     SAAQFVHDQRDAIALVASQDGRFTVFAWSPCAVGVHAHRVEALLL"
     gene            complement(5275..>5565)
                     /locus_tag="AC16CY_12065"
     CDS             complement(5275..>5565)
                     /locus_tag="AC16CY_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354572.1"
                     /translation="NYNNKGWISTSAAGGRIKGIPHTDKLRVVERFRRISAKEISYSA
                     TIEDPDIYTRPWTVSFPLTADPEYRIFEYACHEGNYAMRNSLSASRADERAR"
BASE COUNT          903 a         1919 c         1838 g          905 t
ORIGIN      
        1 ggccgagcgc gcctgcgatt tcccgaatct gatccgcgtg ccgcatctcg tgggcggccg
       61 tcagctcgac ccactggtac atcgtgagcg atccgaagat gcgatgttcc gcgctgaccg
      121 agccgagggc cagtccgtct gcgggggaga cggcctgccg gacggcgtcg cgcgccgcgt
      181 ccagttcggc agccgccgcc gccgcgtcaa ggccgcccgc cggccgcaac gcttcccgcg
      241 cctcgcgcgc ctcgccgcgg tcggccatgc gatgcgcgat ggccggcggg agcggatccc
      301 gctgccggtc ctccgggccg agcccggcga cgcgcgcctc cgcgatcgcg tcggccatcc
      361 gctgggcgaa ggaacgatcg acgagcgcca gatgctcgag cacctcggcc accgaccagc
      421 gcccgggcgc cgggcgagtg cggcgcaggc cttctggcac gccggcaacg gcggcgctga
      481 ctgcgccgcc cgccgcgtcg agacgcccga ggacctctgc tagatgcgga tgcacgccag
      541 aatgttactt gtcctgcggg ttgacggtct tgaaggtcac cgcggcaacg gccgcgcctg
      601 cgaaatcggc cacgagatag atccagaggt tggacacggc ggccagaccc atcgccgtcg
      661 ccccgacggc cacagccgga ttgaacgctc cgccggacac accgccgacg gcgaacgctc
      721 cggtcatcac ggtgaaaccg atcgcaaggc cgtagaagga gttgccgctc gtccccttcg
      781 cggtcgccac gttcagcacc acccaggcca gggcgaaggt aaacaggaat tccgccagga
      841 gcgcctgagc tacgtcgagc gaccgttccg tgacctgggc cattgccgaa cccttcagca
      901 cgccgacggc ggcggcggca atcaggccgc cgacgacctg cacccccatg tagatcggca
      961 cgtccctgga gggacatttc ccgcgcagcc agacggccag cgtgacggcc gggttgtagt
     1021 gggcgcccga cacgtgcccg ccggcaaaca ccatcaccat caaggccgcg ccgatcgcaa
     1081 gcggcgcgag cgaccctgca ccgggttcaa tcaccgtcat gccaatggtg agcaccagga
     1141 aaaacgtgcc gacacactcg acgacgtatt tgcccatacg cgtgcaacct cctcggccca
     1201 ttccgggcgg cgcaggctac catagaggaa gtcctggctg ctaaagattt gcgccggaac
     1261 ggccgagacg aaagatcgag ccctctactc gaagtgactc tgggaagaac catgcacgta
     1321 cgccggctcg tgagtcttac gcttctaccg ctgctcgcgt ttctgacgtg cgggccgacg
     1381 gccgcgcagt ccgacgggac gcaggccacc ttcaagtccg gcgttgacct cgtctcggtc
     1441 gcggccaccg tccgcgacaa aaaggggcgc gtcgttcgat ccctcacgtc caaggacttc
     1501 atcgtcacgg acgaggggca gcggcggacg atcatcggga tgcaagccga cgacggatct
     1561 cccgccaacc tcgcgttgct cgtcgacggc agcgggagca tgaaggtggg gacgtcaggc
     1621 gacgcgtcgc ggctgatttg tcacatgatc ctgaccaggc tgcggcgcga tcaggatcgg
     1681 gcggcgctca tgagcttcga tacgcgcctg ctcacgctcc gcgagttcac ggccgagttc
     1741 gaccacatcc agacgggact cggagaagtc gccccgttcg gatcgacatc gatctacgac
     1801 gcgatcgccg gcacggcggc tgccgtggcc aagggtaccc agaaacgtcg cgccgtcgtc
     1861 gtcatcaccg acgggtggga caacgcgagc acgtacacgc cgcagcaggt ggcggcgatt
     1921 gcgagcacga tcgacgtgcc cgtgtacgtc tttgccgtgg gcgatctgaa cccggagacc
     1981 gtggcgaacg agaagaccgt ggccgagcga cgcagcgacc tcttcgacct ggcgcgcgcg
     2041 acgggcggag accacttctt ctccggcagc ccgtcgatcg ttcaccaggc ggcgacgcgc
     2101 ctgctcgacg agctccatca tcagtacctg atcgccttcg agccggcgac ggccagcggc
     2161 ctgcggagaa tcgaggtccg gacgcgcaac gatgatcaca acgtccgctc ccgccggtgg
     2221 tacgcggccg cgaactgatc ccgatcattc cagacacgta cgctaaccac agagtcacag
     2281 agagcacagg ggttgctctc tcgcaagaga gcatctctgt gtcctctgtg tctctgtggt
     2341 tggcgtccgt gacgtagact ccgaacgtca tgttctggca cggctgcaac tacccgtggt
     2401 caaccgacgg gacctcgagt tgctacggcc tcgacttcgg ggccaacgta tggggcaccc
     2461 acgccggcgt ctccacccgg cgtcaacgga tcgagtccga tttctcgcgg atggcgtcgc
     2521 tcggcttcac cgtggcgaga tggttcgtct ttgccgacgg tcggtcgggc atcacgtatg
     2581 acgagggcgg ccggccggcc ggccccgacc cccatctgtt ctcggacgtc gacgccgcgc
     2641 tcgagatcgc ccgcggcgcg aacatgcgcc tgaatctcgt gctgctcgac catcgctgga
     2701 tgttcgacga ggtccccgac gtgatcgcgg atccggcaac cggcctgctt ctcgagacgc
     2761 ggctgcccca cggccgcgcg cacgtgctcc gggtgcagga gggtcgcgac gcgctgatga
     2821 gaaccgtcct cgaaccgctc gtgcggcggt acggccgcaa gggggaacgc gccgatctgg
     2881 gtccgcatgt cttcgccttc gagttcatga acgaacccga cttcgtcgtc gaggagtggg
     2941 aacgcgatgt cagcgctcac gtccggcgcc cactcccctt cgaatcgctc gcggagctcg
     3001 tcgcggggct gagcgcgatc gtccacgagc acaccggcgc gttcaccacg atcgccgccg
     3061 cgcggctgca caacctgtgg gcctgggacg acgaccagct cggtctcgac gtgttgcagg
     3121 tgcactcgta cccggacacc cggcatccgg aacgcgacgc cgacgtgtta ggcctgccgg
     3181 cggcctcgct cggcgtgagg cgcccggtca tcctgggcga gtttcccgcc aacggcccgc
     3241 ggcagcatcc cgctgactcg gctcgtccac cgaccgaact cgaacggtac ttggagtttg
     3301 ccctgacggc ggggtacgcc ggcgcgtggc cctggagctt cagcggaacc gacgcctacg
     3361 ggcgtcttcc cgaggaccct ctgcgcgcgt tcgcgcggcg ccaccccgac ctcgtgaacc
     3421 cgcgagcgct cctggaaccg ggggggcccg caccgacgta agtacattgt cggaggcttt
     3481 accgcatgga cctgatcacg ctggggcgca cgctcggctt ctccttcgcc gctggcgtga
     3541 acctctatgc cacggtggcg atcctggggc tggcgtcgcg gtacggctgg gtcgagcttc
     3601 cggcgcagtt tcaggtattc aacaacgacg tcgtgatcgg cgcggccatc gtcatgtatg
     3661 ccgtcgagtt cttcgccgac aagatcccgt acttcgattc cgtgtgggac atgatccaca
     3721 cgttcatccg cccagtcggc ggagcgctca tcgccgtcgc cacgctgggc gacgcgtcaa
     3781 cacccgtcga aggtctcgtc gcgctgctcg gcggcacgat cgccgcgagc agtcacctta
     3841 ccaaaaccag cacgagggcc atggccaaca cgagtcccga gccgctgtcg aactgggcgc
     3901 tcagcctcgg cgaggacgtg ttcgtggtgg ggctggggta cctggcgctg aagcacccga
     3961 tcgcggcgct cgtcgtcgcg gccgtcctgc ttggagcgat cgcggtcttt gccgccgtca
     4021 tcatccggac ggtgcggcgc tggttcttcc gacggcagat cccgagcccg gcgggctgag
     4081 cccgggacac gtgggcgaga gcgtagcccg aggtatgctg gccgtgtgag ccgccacaag
     4141 catcccgcgg cgcaactggt cgcgccgaag gtcgaggcct acttcgctca acacctcgag
     4201 gccgctcgac aacgcggtga cgagcacgtg gcgccgctcc ccacggcgga cgcgatcgag
     4261 accatgatcg acgcggcgtt ctgggcgagc ttgagacgcg aggaaggcta cgtcccgaag
     4321 atctcgctcg cgttcctccc tcccgacctc gacccgtacc cgatgctcct cgagcgtccg
     4381 cttgccctcg accccggcgt tctgaccaag gtggctccgg tggtcgatcg agccgggatc
     4441 catctcggcg tgtggccccg aaacggggag ttgagcgtgt ggggaacgac gcacacgatt
     4501 cctccgctct gtttcgtcct cgaggtggtc gagccggggc ttctcgtggt gaaacatcac
     4561 cgcggcgagc agtccgggaa gtacgtcaac gtggcggtac tggaaggcga tcagatcaag
     4621 atcgttgacg agacggcctc gagccttccc gactgccccg cgctcctgac gtcgctcctc
     4681 ggcttcgatt caccggcatc gtgggccggt ccggtcaacg tgctggtgca gcttgccgtc
     4741 tcgatgcgca cccaccgccg cggcgggacg ctgctcatcg ttcccgccgg caccgatcga
     4801 tggcgcgaat cgatcgtgag cccgatcccg tacgccatcg agcccccgta ccgcgagctt
     4861 gccgacttga tccgcgaggg gcgcagcgaa cgccgctccg acacgtggca ggaagcactt
     4921 cgggagacgg tggacgcgat tgcggggctg acggccgtcg acggcgcgac gctcttgacc
     4981 gactcctacg agctcctggc attcggcgcg aagatcgccc ggcggcgggg agcgccacgg
     5041 gtggacgaga tcacgctgac cgagccgatc gaaggaggta cggcgtcggt gatctcaccc
     5101 gtgcagcttg gcgggacacg gcatctttcg gcagcacagt tcgtgcacga tcaacgggac
     5161 gctatcgcgc tcgtcgcctc gcaagacggg cgattcaccg tgttcgcgtg gtcgccgtgc
     5221 gcggtgggcg tgcacgctca ccgggtggag gcgttgttgt tgtgatctgg cccgctagcg
     5281 cgcccgctcg tcggcgcgcg acgccgagag cgagttccgc atcgcgtagt tcccctcgtg
     5341 gcacgcgtac tcgaagatcc ggtactccgg atccgcagtc agcgggaacg acaccgtcca
     5401 cggcctcgtg tagatgtcgg gatcttcgat ggtggctgaa tagctgatct ccttggcaga
     5461 gatacgccgg aagcgctcga cgacgcgaag cttgtcggta tgcgggatgc ccttgatgcg
     5521 tccgccggcc gcgctggtcg aaatccaccc cttgttgttg tagtt
//
LOCUS       DBNGSH010000524         4792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2426871, whole genome shotgun sequence.
ACCESSION   DBNGSH010000524 DBNGSH010000000
VERSION     DBNGSH010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2426871"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="AC16CY_12070"
     CDS             complement(<1..229)
                     /locus_tag="AC16CY_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354573.1"
                     /translation="MSTGQLLVRVVMFVDISGSSRLYKEMGDAAAVLRVRGCLSRLSQ
                     VVDEHGGRTVKNMGDGLMCDFPAADKALLAAE"
     gene            460..978
                     /locus_tag="AC16CY_12075"
     CDS             460..978
                     /locus_tag="AC16CY_12075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3354574.1"
                     /translation="MGDDERSLIEAAQADPKRFLELYDRHVDRVYAYVSRRVRSRAAA
                     EDITSAVFEQALANLLSFEFRGAPFAAWLFRIAANALTDHWRRDGRDVHDPPTDVPDE
                     REHRDLELRVSLFQLVDRLPEMQRRVIELRFIEGRSIRDVAAALDRSEGAVKQLQLRA
                     LENLRKSMGHHG"
     gene            971..1921
                     /locus_tag="AC16CY_12080"
     CDS             971..1921
                     /locus_tag="AC16CY_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3354575.1"
                     /translation="MAEEPLIDRLDRIIDALVAGGDATAGLRDDEVAPLTRVAADLRH
                     YPRREFTRELRAQLEGRTRMATALTAPNIRTGFTTVTPYVWVKDRGFADFVVRVFGAV
                     ETSVADNPGHGIHRELRVGGSMLMIGETGEGTTPARPVAFHVFVDDTDAVFAKALQAG
                     GTSLGEPADRPYGERSGFIRDPYGNHWYIATSLGPSAVPAELRTVTPFLHAQNVGEYI
                     GFLERAFGAVEEARHEVGGGAVPYARVRIGDAAVEMGGPADPMPGAYFLYTADADLAY
                     ERAVAAGAKPLSPPAVRPSGDRIGFVEDPVGNHWYIAKSS"
     gene            1958..4630
                     /locus_tag="AC16CY_12085"
     CDS             1958..4630
                     /locus_tag="AC16CY_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3354576.1"
                     /translation="MTLSPGSRLGPYQITTQLGAGGMGEVYRASDTKLGREVAIKVLP
                     ESVARDPDRLARFDREARALAALNHQNIAHIYGLEDAEGMKALVMELVEGPTLADRIA
                     AGPIPVDEALPIAKQIADALEAAHEQGIIHRDLKPSNVKVRPDGAVKVLDFGLAKALE
                     PTGALPTSQSMSPTITTPAMTQAGMILGTAAYMSPEQARGRPVDRRADIWAFGCVLFE
                     MLSGRSAFGDASVTETLAAVLERQVDWSVLPLSTPPGVRRVLRRALEKDPKRRLHDIA
                     DARIEIDDNYVAPVSTSSTSARGRGAMLPWMTVTGVIAILVGGTAWYLGRRAPVTAPA
                     AVTRFVVETEGPVPTDVEGVVALSPDGRRLAYIAAPAGQRRLYLHELDQFESRPVPGT
                     EGAADPAFSPDGNWIAFTAAGRVKKVSVTTNTVESLTEARYDRGAYLSWESNDSLLIG
                     SAGRATGIWRVPATGGVPTAVTRMHAGETHKNPAILPGGKGLLYSAEIEQGGQLIAES
                     LETGERHNLGTGGRARYLSTGHLVYTRGSGLFAVPFDPVRLEPLGKPTAVLQGIGGTT
                     QESGAVALSDSGSIAYLPDGAADRQSAFVWVDRGGVERPTSITDLSLWRPRLAPDGRR
                     VAVVIRSDAGQGQNLGDIWIYDLVRGTSNRITFDGRSTFPAWSPDGGRLAYSADGGDG
                     RYQLHIKTFSGAGSDVTIATNRGSNHPFSWSPDGRFLATVSVDTTTANDIWVLPVDDP
                     SRWRPFVNSMQGEGGPTFSPDGRWIAYASDQSGRTEIYMRPYPGPGEEVTISTDGGNE
                     PIWVHKTGELFYRRGDAVMVVGITTTPTVSVGKPRVVVERPYMRSGAFFPNYSVSSDG
                     QRLLMIKATGREVPARFHVVLNWAEELKSLVPAK"
BASE COUNT          821 a         1555 c         1647 g          769 t
ORIGIN      
        1 cctccgcggc cagcagcgcc ttgtcggcgg ccgggaaatc acacatgagg ccgtcgccca
       61 tgttcttgac cgtcctgccg ccatgctcgt ccaccacctg gctcagccga ctcaggcagc
      121 cgcgcactcg gagaactgcc gcggcgtctc ccatctcttt gtacaggcgg gagctgccgc
      181 tgatgtcgac gaacatcacg acgcggacga gaagctggcc cgtgctcacg tggggcgggt
      241 catccagggc atggggatct attggactgc atcaactgaa cctgtaacgc caacgacagc
      301 ctttcgaccg cggtttcgcg ggccaaccaa cgggcggccc gcctggaaat cacaaggacg
      361 tcccacgagc tgcatgatag ccagccacat ttgcccgcca caagggccgt aaccttcggg
      421 tgggttcgtc cgttattgtg atcgagcgat gcaccggacg tgggagacga cgagcggtcg
      481 ctgatcgagg cggcacaggc ggatccgaag cggtttctcg agctgtacga ccgccacgta
      541 gaccgcgtgt acgcgtatgt cagccgccgg gtcaggagcc gcgcggcggc ggaggacatc
      601 acgtccgccg tcttcgagca ggcgctggcg aacctgctgt cgttcgagtt tcgcggggcg
      661 ccgtttgcgg cctggctctt tcgcatcgcc gcgaatgcgc tgaccgacca ctggcgccgg
      721 gacgggcgcg acgtccacga cccgcccacc gacgtgcccg acgaacggga gcaccgcgat
      781 ctcgagctgc gcgtaagcct ctttcagctc gttgaccgcc tgcccgagat gcagcgccgg
      841 gtcatcgagc tgcggttcat cgagggccgg agcatcaggg acgtcgccgc ggcgctcgac
      901 cgctcggagg gggcagtcaa gcagctgcag ctacgcgcgc tcgagaacct gcgcaagagc
      961 atgggacacc atggctgaag agcccctgat cgatcgtctc gaccggatca tcgacgccct
     1021 ggtcgcgggg ggcgacgcca ccgccgggct gcgcgacgac gaggtcgcgc cgctgacacg
     1081 ggtggctgcg gacctgcggc attacccgcg ccgcgagttc accagggaac tgcgggcaca
     1141 gctcgaaggg aggaccagga tggccacggc acttactgca ccgaacatcc gcaccggctt
     1201 cacgaccgtc acgccttacg tgtgggtcaa ggaccgcggt ttcgccgact tcgtcgtgcg
     1261 cgtgttcggc gcggtcgaga cgagcgtcgc cgacaaccct ggccacggga tccatcgcga
     1321 gctgcgggtc ggcgggtcga tgctcatgat cggggagacc ggggagggca ccacgccggc
     1381 gcgcccggtc gcgttccacg tgttcgtcga cgatacggat gctgtctttg ccaaggcgct
     1441 gcaggccggc ggtacgtcgc tcggtgaacc ggccgatcgt ccctacggcg agcgatcggg
     1501 attcatccgc gatccctacg gcaaccactg gtacatcgcc accagtctcg ggccgagcgc
     1561 cgttcctgcg gagttgcgca cggtcacgcc gttcctgcac gcgcagaacg tcggcgagta
     1621 catcgggttt ctcgagcgcg cgttcggcgc ggtcgaggag gcgcgacacg aggtgggggg
     1681 cggggcagtg ccctatgcgc gcgtccgcat cggcgacgcc gcggtcgaaa tgggagggcc
     1741 cgccgatccg atgccgggtg cgtatttcct ctacaccgcc gatgccgacc tcgcgtacga
     1801 gcgcgcggtg gcggccgggg cgaaaccgct ctcaccgcca gccgtacggc cgtccggcga
     1861 tcgcatcggc ttcgtggagg acccggtggg caaccactgg tacatcgcga aatcgtcctg
     1921 aggcgcgggg tgtcgcgccc gtataatccc gtgtgtcttg acgctctctc ccggctctcg
     1981 cctcggcccg taccaaatca cgacgcagct cggcgcaggt ggaatggggg aggtgtaccg
     2041 cgccagcgac acgaagctcg gacgagaggt cgccatcaag gtgctcccgg agtccgtggc
     2101 acgcgaccct gaccgcctgg cccgcttcga ccgcgaggcc cgcgccctcg cggcgctcaa
     2161 tcaccagaac atcgcacaca tctacgggct ggaagatgcc gaggggatga aggcgctggt
     2221 aatggaactg gtcgagggcc ccacgctcgc cgaccgcatc gcggcagggc ccattcctgt
     2281 agacgaggcg ctgccgatcg cgaagcagat tgccgacgcg ctcgaggctg cccacgagca
     2341 ggggatcatt cacagagatc tgaaaccgtc caacgtgaag gtgcggccag acggcgcggt
     2401 gaaggtgctg gacttcggcc tggcaaaagc cctcgaaccg accggcgcgc tgccgacaag
     2461 ccagtcgatg tcgccgacga tcacgacacc cgcgatgacg caggccggga tgattctcgg
     2521 taccgcggcc tacatgagcc ccgaacaggc gcgcggcaga ccggtggaca ggcgcgccga
     2581 catctgggcg ttcggctgcg tgctgttcga gatgctctcc ggccggagcg cattcggcga
     2641 cgcgagcgtc accgagacac tggccgccgt tctcgagcgg caggttgact ggagcgtgct
     2701 tcccctttcg acgccaccgg gcgtacgcag ggtattgcga cgcgcgctcg agaaggaccc
     2761 caagcggcga ctgcacgaca tcgcggatgc gcggatcgag atcgacgaca actacgtcgc
     2821 gcccgtgtca acgtcgtcca cgagtgcacg cgggcgcgga gcgatgctgc catggatgac
     2881 agtgacgggc gtgattgcca tcctggttgg agggacagcc tggtacctgg ggcgacgggc
     2941 accggtaact gcccccgccg ctgtcactcg tttcgtcgta gagacagagg ggcctgtgcc
     3001 gaccgacgtc gagggcgttg tcgccctctc tcccgacggg aggcggctcg cgtatatcgc
     3061 agcgcctgcc ggccagcgcc gactctatct ccacgagctg gatcaattcg agagcaggcc
     3121 ggttcccggc acggagggcg ccgccgatcc cgccttctct cccgatggga actggatcgc
     3181 cttcaccgcg gcaggccggg tgaagaaggt ttcggtgacg accaatacgg tggagtcgct
     3241 gacggaagct cgatacgacc ggggtgccta cctgagctgg gaatcgaacg actcgctgct
     3301 cattggctcg gcgggtcgcg caacggggat ttggcgagta ccggcgacgg gaggtgtgcc
     3361 gacggcggtg accaggatgc acgcaggaga gacacacaag aacccggcca tccttccggg
     3421 cggcaagggg ctcttgtaca gcgcggagat tgaacagggg ggccagttaa tcgccgagtc
     3481 gctggagact ggggagcggc ataacctggg cactggcggc cgcgcccggt acctctcgac
     3541 gggacacctc gtgtataccc gtggcagcgg gctcttcgcc gtgccgttcg acccggttcg
     3601 actcgagcct ctcggcaagc ccactgccgt actccaaggc atcggtggga cgacgcagga
     3661 gtcgggggcg gtagcgctgt ccgacagcgg atcgattgcg tatcttccgg atggtgccgc
     3721 ggatcgccag agcgctttcg tgtgggtgga tcgaggcggt gtggagcgcc cgacctctat
     3781 cacggatctc agcctgtgga ggccgcggct ggcccccgac ggtcgacgcg tggccgttgt
     3841 aatccgcagc gacgccgggc aggggcagaa tctcggggac atctggatct acgacctggt
     3901 gcgcggtacg agcaatagga tcaccttcga cgggcgcagc accttccccg cgtggtcgcc
     3961 ggacggaggc cggctcgcct acagcgccga cgggggagat gggcgatacc aattgcacat
     4021 caagacgttc agtggcgcgg gctcggatgt gacgatcgcg acgaaccggg gcagcaacca
     4081 tccattttcc tggtcgcccg acgggcgctt cctggccacg gtgtccgtcg ataccacgac
     4141 tgccaatgac atctgggttc ttccggtgga cgatccgtct cggtggcggc cattcgtgaa
     4201 ttctatgcag ggcgagggcg ggccgacctt ttcacccgat gggcgatgga tcgcctacgc
     4261 ctcggatcag tcgggtcgca ccgaaatcta catgcgtcca tacccgggtc ccggtgaaga
     4321 agtgacgatc tcgaccgacg gcggcaacga gccgatctgg gtgcacaaga ccggagagct
     4381 gttctatcgc cgtggcgacg cggtgatggt cgtcggcatc acgaccacac cgacagtgtc
     4441 agtgggcaag cctcgcgtag tcgtcgagcg cccgtacatg cggagcggcg ccttctttcc
     4501 gaactacagc gtgtcatcgg atggtcagcg cctcctgatg atcaaggcga cgggaaggga
     4561 ggtgcccgcg cgcttccacg tcgtcctcaa ctgggccgaa gagctgaaga gtctggtacc
     4621 cgcgaagtga cgcgcggcta cgttgctatc gtcccggctt tccggagacg agtaactcga
     4681 tcgctcgctg gtgcgcagcc acgaccggcg tgacgagcga ggcgtccacg cgctcgagcg
     4741 tgtcttccac ggtgtggaac caggaatgga cgccgagcac cgcgaatgcc gt
//
LOCUS       DBNGSH010000525         2496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2257775, whole genome shotgun sequence.
ACCESSION   DBNGSH010000525 DBNGSH010000000
VERSION     DBNGSH010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2257775"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC16CY_12090"
     CDS             complement(<1..209)
                     /locus_tag="AC16CY_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354577.1"
                     /translation="MRRALPLAAAVLMGSILGAQGRGTPPGLEPRIVLFEAKPAAVRA
                     GQPVLLVWHVENPTGISIEPGIGAVT"
     gene            complement(223..1083)
                     /locus_tag="AC16CY_12095"
     CDS             complement(223..1083)
                     /locus_tag="AC16CY_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354578.1"
                     /translation="MKRDVIRAIATAAIALACAAPAIVGLTAEQGGQGGAAAATPADA
                     PAGPVPRMADGRPDLSGVWWGGADVGAARGRGGGGRGGARGGGARGGTPPATFTSLYR
                     PEAAAKAKTLSDKDDPTLRCVPTAFGTLNVSLYDVGAVGQIISTPKMVVMLTETYHGF
                     RLIPTDGRPHRDDVPPAYRGESVGRWEGDTFVVDTRNFTDNTWMSAEGRVSHHSDQLR
                     IVERYRRVDANTLEIEATVEDPGVLTAPWTVPKQTLVLAPFDRIMSLNCSGIETQALM
                     DGAARQSTVK"
     gene            complement(1093..1503)
                     /locus_tag="AC16CY_12100"
     CDS             complement(1093..1503)
                     /locus_tag="AC16CY_12100"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354579.1"
                     /translation="MTRRVRTALAWGVLSLGTAAAAWAHHSFAAAFDMQKPVTVQGTI
                     VQVRLENPHSWFFLDVKDAGGKVERWAFEAGTPSGMIRNGFKPNEIKKGVEVTIRGFR
                     AKDPTQNMGMLRELETSDGKKYGMFGSQQGPGAQ"
     gene            1665..>2496
                     /locus_tag="AC16CY_12105"
     CDS             1665..>2496
                     /locus_tag="AC16CY_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3354580.1"
                     /translation="MAQNGDRESLEWLVRRHSPWIYNIALRMLYRPDDAQDASQEILV
                     KVITRLSTFGGRSSFRTWLYRIAVNHLLTMKQLRQERWTFEAYGLALEALRESELVDR
                     TTPAADTQLWLEEAKIGCTTGLLLCLDREQRLIYILGDIFGLSDRLGAELLELSRDNF
                     RKKLSRARHDLQSFAQDKCGLINPANPCRCSRKTKAFIEAGYVNPQNLLFVRERVQAI
                     RDVAPAVYDNLETAQREYAELHRAHPFQVPPDFVARLRSLLGIMLMVLFVQACRAPAV
                     P"
BASE COUNT          397 a          849 c          800 g          450 t
ORIGIN      
        1 gtgaccgcgc cgatccccgg ttctatggag atgcccgtcg ggttctcgac gtgccagaca
       61 aggagcaccg gttgacccgc tcgcacggcg gcgggtttcg cctcgaacaa cacaatccgc
      121 ggttcgaggc ccggcggggt gccgcgcccc tgcgcaccga ggatcgaacc catgaggacg
      181 gccgccgcca gcggaagtgc gcgtcgcatg tcgccgtctc ccctacttga cggtgctctg
      241 cctggccgcg ccgtccatca gcgcctgtgt ctcgatcccc gagcagttga gggacatgat
      301 gcgatcgaag ggcgccagca ccagcgtctg cttcggcacg gtccacggag ccgtgagcac
      361 gccgggatcc tcgacggtcg cttcgatctc cagggtgttc gcgtccacgc gccggtaacg
      421 ttccacgatc cgcaactggt cggagtggtg agagacgcgt ccttccgccg acatccaggt
      481 gttgtcggtg aaattcctcg tgtccacgac gaacgtgtcg ccctcccaac ggccaaccga
      541 ttcgccccgg taggccggag gcacatcgtc gcggtgcggc cgtccgtcgg tgggaatcag
      601 gcggaagccg tgataggtct ccgtcagcat caccaccatt ttcggcgtcg agatgatctg
      661 ccccacggcg ccgacgtcgt agaggctgac gttcagcgtg ccgaaggcgg tcgggacgca
      721 gcgcaaggtc gggtcgtcct tgtcgctcag cgtcttcgcc ttcgcggccg cttccggccg
      781 atagaggctg gtaaacgtcg ccggcggggt tccgccgcgc gcgccgccgc cacgcgcgcc
      841 gccgcgtccg ccgcctcctc gtcctctggc tgcgccgacg tctgcgccgc cccaccacac
      901 cccgctgagg tccgggcgcc cgtcggccat tcgcggcacc ggccctgcgg gagcgtccgc
      961 aggagttgct gccgccgcgc caccctgccc accctgctcg gccgtcaggc cgacgatggc
     1021 gggcgcggcg cacgcgagcg cgattgcggc cgttgctatg gcgcgaatga catcacgctt
     1081 catggtcgac tttcactgcg cgccagggcc ttgttgagat ccgaacatgc cgtacttctt
     1141 tccgtcggac gtctcgagct cccgcagcat gcccatgttc tgcgtggggt ccttcgcgcg
     1201 gaagccccgg atggtgactt cgacgccctt cttgatctcg ttcggcttga agccgttgcg
     1261 gatcatgccg ctcggcgtcc cggcctcgaa ggcccaccgc tcgaccttgc cgccggcgtc
     1321 cttcacgtcc aggaagaacc acgagtgcgg attctcgaga cggacctgca cgatcgttcc
     1381 ttgaaccgtg acaggcttct gcatgtcgaa ggctgccgca aacgagtgat gcgcccaagc
     1441 cgccgctgcg gtgccgagcg aaaggacgcc ccacgcaagg gccgtgcgga ctcttcgcgt
     1501 catacagacc tccatcgaga gccggtgtcg tcagtattct gccatgaaca tgcggtctcc
     1561 gctgtcacac ttggcttgcg ccgatgtcta aacaggacat gaggacagaa agcccgttct
     1621 tggacgaggc cgccgtcaac cacgatgagg agcggagtgt ccgcatggcc caaaacggcg
     1681 accgggagtc gctggaatgg ctggttcgcc gccactcgcc atggatctac aacattgcgc
     1741 tccggatgct ctatcgcccg gacgatgcgc aggacgcgtc gcaggagatc ctcgtcaagg
     1801 tgatcacgcg cctctccacg ttcggggggc gcagcagctt tcgcacgtgg ctgtatcgca
     1861 tcgcggtgaa ccatctgctg acgatgaaac agctgcgaca agagcgatgg accttcgagg
     1921 cgtacggact cgcgctcgag gcgctgcggg agagcgagct ggttgaccgc acgacaccag
     1981 cagcggatac gcagttgtgg ctcgaggagg caaagattgg ctgcacgacc gggctgttgt
     2041 tgtgtctcga ccgcgagcag cgtctgatct acatcctcgg tgacattttc ggcctctcgg
     2101 atcgcctcgg cgcggagctg ctcgagctgt cacgggacaa cttccgcaag aagctctcgc
     2161 gcgcccgcca cgatcttcag agcttcgcgc aggacaagtg cggcctgatc aatccggcga
     2221 acccgtgtcg gtgctcgcgc aagacgaagg cgttcatcga ggccgggtat gtcaacccgc
     2281 agaacctgct cttcgtccgc gagcgcgtgc aggcgattcg tgacgtcgcg cccgcggtgt
     2341 atgacaacct cgagacggcg cagcgcgagt atgccgagct gcatcgcgcg catcccttcc
     2401 aggtgccgcc cgacttcgtt gccaggcttc gatcgctgct cggcatcatg ctgatggtgc
     2461 tcttcgtcca ggcgtgtcgc gcgccggcag tacctg
//
LOCUS       DBNGSH010000526         5974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4464491, whole genome shotgun sequence.
ACCESSION   DBNGSH010000526 DBNGSH010000000
VERSION     DBNGSH010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4464491"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1437)
                     /locus_tag="AC16CY_12110"
     CDS             complement(226..1437)
                     /locus_tag="AC16CY_12110"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3354581.1"
                     /translation="MSTIVQKYGGSSVADVGKLKYVAERVMQTARRGHRVVVVVSAMG
                     DTTDELLAMAKQVSPNPDRRELDMLLSAGERISMALLSLAIRELGGDAISFTGSQSGI
                     ITNDRHVDARIIEVRPFRVQDELARGRIVVVAGYQGVSYKREVTTLGRGGSDTTAVAM
                     AAALDAEYCEICSDVDGVYSADPRVVPAARRIGTLSYEETQEMAESGAKVLNAQAVEF
                     AKEKGIAIYARATQGPLPGSDPSADGTVVRKSPPRMPGTVVGVVSERDVLVLDGTAPP
                     ADILALLAERGVPGKQLHVFNDRTTVVISRENLHDEGQLRDALQARFGGRARLADGLA
                     AVSVIGAGINATYENVRTGAGTLGTLGIVPAGTATSSFRTTWMVPDDRVKEAVRALHA
                     RFIEQPRPLLP"
     gene            1685..2344
                     /locus_tag="AC16CY_12115"
     CDS             1685..2344
                     /locus_tag="AC16CY_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354582.1"
                     /translation="MSTRALFGILIAAAMTVMTLAPASAVTHDKLAYLTFTGPVQVPG
                     AILNAGTYQFRLTNPDVSRNVLQVRSNDGSIVYGMFNTIPDSRTAMTRDTVVTFRETP
                     AGVPAAVKTLFYGGENVGYEFVYPKGGPIMRVGVAPQPPITYARTPAVEEPEVAAPVE
                     PVWEPEPEITVETEPMPEPEPVPPAELPRTASPLPLAAAGGLTSLLVGLGLGWLRRRQ
                     G"
     gene            2402..3313
                     /locus_tag="AC16CY_12120"
     CDS             2402..3313
                     /locus_tag="AC16CY_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3354583.1"
                     /translation="MQTLRRAGLLAGLCVVCVSASAQQTVERPPVLSVETDLVTLAVT
                     VVDRHGVFVAGLRQEDFTVYDEGEPRSIQFFTSEDLPATIGLVIDSSGSMRRRSNDVA
                     AAVTAFAALRHPFDELFTVNFNEAVWAGLPPAVAFTSDPDELRSALTTAPPQGMSALY
                     DAVDRALAHLGLGTRDRKALIVVSDGGDNASTQTFDAVLEHARRSKATIYTLSLFDPD
                     DGDARPRVLRALARETGGASFAPRRPEDVTGAFMEIARELRSGYTIGFRPPGTPGGGF
                     RSIRVVVDTDARRRLIVRTRAGYYAGP"
     gene            3300..4316
                     /locus_tag="AC16CY_12125"
     CDS             3300..4316
                     /locus_tag="AC16CY_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HIR3354584.1"
                     /translation="MRDPSDRQTRNRGATVLHWAQRLLVTVGAVTLAWCALIVTDATL
                     AQRRARHSLEVASRAAVASPSAMATPLPALPAMVRPTALRRGAAIAGLSIPRVELSAV
                     VLHGSDTETLRRGPGHLENTALPGEPGNAVIAGHRDSFFRPLRHVEIGDDIFVDTPHA
                     RLRYRVTSLHVVDSHDLSVLQSTDDATLTLITCYPFQVFGAAPDRFVVRASLVTDVSE
                     ARTTHVPAPRHPMTMTTIEAQAARRPAVLNGPATHDDAMAVRHAVERFRRTYNARLVS
                     HQDVRPGGPLDLQSCDVEIADNRATATCGTAPSPGGHDDSSAWTVALERTGQGWAIRS
                     IVAD"
     gene            complement(4373..>5974)
                     /locus_tag="AC16CY_12130"
     CDS             complement(4373..>5974)
                     /locus_tag="AC16CY_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3354585.1"
                     /translation="VGIAYAEASGVPGIYGLYATIVPLLVYAIFGPSRILVLGPDSSL
                     AAVILAVVAPLSAGDPQRALALAGTMAVVSGVVCVGAGLARLGFVTELLSKPIRYGYM
                     NGIALTVILSQLPKLLGFSIEADGPLRQVQAIAEGMMDGRTNVPTLAIGVGALILILV
                     LKRRPRVPGILISVVGATIVVAALDLSERAGVTVLGPLPQGLPRFAFPLIAFDDLVPV
                     LTGGIAVALVSFADTSVLSRTYAARLRTVVDPNREMVGLGAANLAAGFFQGFPISSSS
                     SRTPVAEAAGSRSQLTGVVGACAIALLLVLAPNLLQDLPNTALAAVVIASAIGLAEVK
                     DLRRIYRIQQWEFWLSMVCFVGVAVLGAIPGIALAIVIAVIEFLWDGWRPHSAVLGRV
                     DRVKGYHDVTRYPQARLIPGLVLFRWDAPLFFANAELFHDRAMAAVSGSPTPVRWLVV
                     AAEPVTSVDVTAADVVAELDDALHASGIELCFAEMKDPVKDKLKRFGLFTRLGEEQFF
                     ATVGEAVTAYLARHQVAWEDWQQRP"
BASE COUNT         1026 a         1999 c         1995 g          954 t
ORIGIN      
        1 tcaaatggcc ttcggcctcg atcgtgtccg tgactgacat caggccatca ttctagacca
       61 cggcgtgaca ttggtgattt ctgattggtg atttctgaat cgttgttgat tgaagattgt
      121 tgattggcga ttgggctctc aatcaccaat ctcaaatcac aaatcatcaa cgaatcgaca
      181 atcaccaaaa tcaccgatca caaatcgact ggcgtctcgt tctgttcagg gcagcagtgg
      241 ccgcggctgt tcgatgaaac gcgcgtggag ggcgcgtacc gcctctttca cgcgatcgtc
      301 aggcaccatc caggtggtgc ggaaggagga tgtcgccgtg cccgcgggaa cgatgccgag
      361 cgtgcccagc gtcccggctc cggtgcgaac gttctcgtag gtcgcgttga tcccggcgcc
      421 gatcacgctg acggcggcga gcccgtcggc gagccgcgcc cgccccccga accgcgcctg
      481 caaggcgtcc ctgagctgtc cctcgtcatg caggttctct cgcgagatca cgacggtcgt
      541 ccggtcgttg aagacgtgca gctgtttgcc tggaaccccg cgctcggcga gcagcgcgag
      601 gatgtcggcg ggcggggcgg ttccgtccag gacgagcacg tcgcgctcgc tgacgacgcc
      661 gacaacggtg ccaggcatac gcggcggact cttgcggacg acggtcccat ccgccgaggg
      721 atccgagccc ggaagcggcc cctgtgtggc gcgcgcgtag atggcaatac ccttctcctt
      781 cgcgaattcg accgcctgcg cgttcagaac cttggcgccc gactccgcca tttcctgcgt
      841 ctcttcatac gagagcgtcc cgatccgccg ggcagccggg acgacccgcg gatccgcgga
      901 gtagacgccg tccacgtcgg agcagatctc gcaatactcc gcgtcgaggg ccgccgccat
      961 ggccaccgca gtcgtgtcgc tgccgccgcg accaagtgtg gtgacttccc tcttgtacga
     1021 aacgccctga taaccggcga ccacgacgat acggccgcgc gccagctcgt cctgcacgcg
     1081 gaacgggcgg acctcgatga tgcgcgcatc gacgtggcga tcgttggtga tgatgccgga
     1141 ctggctgccg gtgaaactga tggcgtcgcc cccgagctcg cggatggcga gcgacagcag
     1201 cgccatcgaa atccgctcgc cggccgagag cagcatgtcg agttcacgac gatcggggtt
     1261 cggcgacact tgcttcgcca tcgccagcag ctcgtccgtc gtgtcgccca tggccgacac
     1321 gacgacaacc acccgatgcc cgcgccgcgc cgtctgcatc acccgctcgg ccacgtattt
     1381 gagcttcccg acgtccgcaa cgctgctgcc gccgtacttc tggacgatgg ttgacatgac
     1441 aaatggtacg acgccggaga ggaagacgtg acgcgtcggc cccggcttca gccacggacc
     1501 ggagtgcaat ttgccgcggg gtccgcgcaa aaagtcgcgc cggcccccgc ccccatgggc
     1561 ggatccctgt cgcgcgatgc ggccgggcgc ggccacccgg aggcagggga aatgcatcac
     1621 ttgggttcaa acacgctgga gccgaagggc gccgtctgtc cttcggggaa gctcggaggg
     1681 ttgtatgtct accagagcgc ttttcggcat tctcatcgcg gcagcgatga ccgtgatgac
     1741 gctcgcgccc gcgagcgcag tgacccacga caagctggcc tacctgacct tcaccgggcc
     1801 ggtgcaagtc cccggcgcga ttctgaacgc cggcacgtat caattccggt tgacaaaccc
     1861 cgatgtcagc cgaaatgtcc ttcaggtgag gagcaacgac ggctcgatcg tgtatgggat
     1921 gttcaacacg attccggaca gccgcacggc gatgacgcgt gacacggtcg tgaccttccg
     1981 cgaaacgccg gccggtgtgc ctgccgccgt aaaaacgctg ttctacggcg gcgagaatgt
     2041 cgggtacgaa ttcgtctatc cgaagggcgg gccgatcatg cgtgtcggcg ttgcaccgca
     2101 gccgccgatc acgtatgccc ggacgccggc tgtcgaggag ccggaggtcg cggctccagt
     2161 agagccggtc tgggagcctg aaccggaaat caccgtggag acggagccga tgccggagcc
     2221 ggagccggtt cctcctgcgg agcttccgcg gacggcgagc ccactgccac tcgctgcggc
     2281 tggcgggttg acctcgcttc tcgtgggact cgggctcggc tggcttcgcc gccggcaggg
     2341 ctgagtgagc gggcggcacg gccgggcgga accgcccggc cgtcgctgct gggggttgga
     2401 catgcaaacg ctgcgacgtg cggggctgct tgcgggctta tgcgtcgtgt gtgtctcggc
     2461 ctccgcgcag cagaccgtcg agcgccctcc agtgctttcg gtcgagacgg acctggtcac
     2521 gctggccgtg accgtcgttg accggcatgg tgtgttcgtt gccggccttc gtcaggagga
     2581 tttcacggtc tacgacgaag gtgagccacg gtcgattcag ttcttcacga gtgaggatct
     2641 tccagcgacg atcggcctgg tgatcgacag tagcggcagc atgcgacgcc gatccaacga
     2701 cgtcgcagcc gcggtgacgg catttgccgc actgcgtcat cccttcgacg agctcttcac
     2761 ggtgaacttc aacgaggcgg tctgggcggg ccttccgcct gctgttgcgt tcacgtcaga
     2821 tcccgacgag ctccgttcgg cgctcacgac ggctccaccg caggggatga gcgcgctgta
     2881 cgacgcggtg gatcgtgccc tcgcccatct cgggctgggc acgcgcgacc ggaaggcgtt
     2941 gatcgtggtc agcgacggtg gagacaacgc gagtacgcag acgttcgacg ccgtgctgga
     3001 gcatgcgcgc cggtcgaagg ccacgatcta cacgctaagc ctgttcgacc ccgacgacgg
     3061 cgatgcccgg cctcgcgtgt tgagagcact cgctcgcgaa actggcggtg cctccttcgc
     3121 gcccaggcgc ccggaggacg tgacgggcgc cttcatggag attgcgcggg agctcagaag
     3181 cggatacacc atcggcttcc gccctcccgg cacgccagga ggcggctttc gatcgattcg
     3241 cgtggtcgtc gacacggacg cccggcgccg gctgattgtc cgcacgcggg cgggctacta
     3301 tgcgggaccc tagcgaccgg cagacacgaa accggggcgc gaccgtgctg cactgggcac
     3361 agcgcctcct cgtcacggtc ggcgccgtga cgctggcatg gtgcgcgctt atcgtgaccg
     3421 acgcgacgct tgcacagcgc cgcgcacgcc actcgctcga ggtggcgtcg cgcgccgcag
     3481 tcgcatcgcc ttccgcgatg gcgactccgt tgcctgcact gcctgcaatg gttcggccga
     3541 cggcccttcg tcgaggcgcg gctatagccg gtctgtcgat cccgcgcgtt gagttgtctg
     3601 ccgtcgtgct gcacggctcg gataccgaga cgctccgccg cggccccggg cacctcgaaa
     3661 acaccgccct tcccggcgag cccggcaatg cggtcatcgc ggggcatcgt gattcgttct
     3721 tccgaccgct gcggcacgtc gaaatcggcg acgacatctt cgtggataca ccgcacgctc
     3781 gtctccggta ccgcgtcaca tcgctgcatg tggtcgactc acacgacctc agcgtcctcc
     3841 aatcgaccga cgacgccacg ctgacgttga ttacctgtta tccgtttcag gtattcgggg
     3901 ccgcgccgga tcgattcgtc gtgcgtgctt cgctcgtgac cgacgtgtcg gaggcccgca
     3961 cgacgcatgt gccggcgccg cgccacccga tgacgatgac gaccattgaa gcgcaggccg
     4021 cacgccgccc ggcggtcctg aacggcccgg caacccacga cgacgcgatg gccgtacggc
     4081 acgccgtcga acggttccgg cgcacgtaca acgcacgcct cgtcagccac caggacgttc
     4141 gccctggtgg accactggac ctgcagtcct gcgacgtgga gatagcggac aatcgtgcga
     4201 cagcgacctg cggaacagcg ccttccccag gcggccatga cgactcgagc gcctggaccg
     4261 tcgcactgga gcgcaccggc cagggctggg cgatcagatc gatcgtcgcg gattgatccc
     4321 gcctgcgcgc gacggctgtc gagggtgttc cgcgtcgttg gcgcggcaaa ggctacggcc
     4381 gctgctgcca gtcctcccac gcgacctgat gacgggcgag gtaggcggtg accgcctcgc
     4441 ccaccgtcgc gaagaactgc tcctcgccga gcctcgtgaa cagcccgaag cgtttcagct
     4501 tgtccttgac gggatccttc atctccgcaa aacagagctc gatcccggac gcatgaaggg
     4561 catcgtcgag ttcggcgacg acgtcggcgg cggtgacgtc cacgctcgtc acgggctcgg
     4621 cagccacgac gagccagcgc acgggcgtcg gggaaccgga cacggcggcc atcgcgcggt
     4681 cgtgaaagag ctcggcgttg gcgaagaaca acggtgcatc ccaccggaac agcacgagcc
     4741 cgggaatcag gcgggcctgc ggatagcggg tgacgtcgtg atagcccttc acgcgatcga
     4801 cgcggccgag caccgctgag tgcggccgcc agccgtccca gagaaactcg atcacggcga
     4861 tcacgatcgc cagcgcgatg ccggggatgg cgcccaacac cgccactccg acgaagcaga
     4921 ccatcgagag ccagaactcc cactgctgga tgcggtagat acgccgcaga tctttcacct
     4981 cggcgaggcc gatcgccgaa gcgatcacga cggcggcaag cgccgtgttc ggcaggtcct
     5041 gcaggaggtt cggcgccagt acgagcagca gcgcaatcgc gcacgcgccg acgacgccgg
     5101 tcagctgact cctggagccg gccgcttcgg cgacgggtgt tcgcgacgaa ctgctgctga
     5161 tcgggaagcc ctggaagaag ccggccgcca ggttggcggc tccgagaccc accatctctc
     5221 gattgggatc gacaacggtg cggaggcggg ccgcgtaggt gcgggagagg acgctcgtgt
     5281 cggcaaatga cacgagcgcc acggcgatgc cgccggtgag aacgggcacg aggtcatcga
     5341 aagcgatcag cgggaaggcg aagcggggca agccctgggg caacgggccg agcaccgtga
     5401 ctcccgcccg ctcggacagg tcgagcgcgg caacgacgat ggtcgcgccc acgacgctga
     5461 tcaggatccc cggcacacga gggcgtcgct tgagaacgag gatcaggatc agcgcgccaa
     5521 cgccgatcgc cagcgtcggg acgttcgtcc tgccgtccat catcccctcc gcgatcgcct
     5581 ggacctgccg caggggaccg tccgcctcga tcgagaagcc gagcagcttg ggaagctggc
     5641 tgaggatcac cgtcaacgca atgccgttca tgtagccgta gcggatcggc ttcgagagca
     5701 gctccgtcac gaaaccaagc cgggccaggc cggcgccgac gcagacgacg cccgagacaa
     5761 cggccatcgt gccggccagc gcgagcgcgc gttgagggtc gcccgcagag agcggcgcga
     5821 cgacagccag gatcacggcg gcgagcgagg agtcggggcc gagcaccagg atccggctcg
     5881 ggccgaagat cgcgtagacg agcagcggca cgatcgtggc gtacaggccg tagattcccg
     5941 ggaccccgga ggcttcggcg taggcgatgc cgac
//
LOCUS       DBNGSH010000527         7942 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1748379, whole genome shotgun sequence.
ACCESSION   DBNGSH010000527 DBNGSH010000000
VERSION     DBNGSH010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1748379"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /gene="rpsB"
                     /locus_tag="AC16CY_12135"
     CDS             complement(<1..728)
                     /gene="rpsB"
                     /locus_tag="AC16CY_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR3354586.1"
                     /translation="MSGIAMKDLLEAGVHFGHQTKRWNPKMREYIFGERNGIYIINLN
                     KTVKKFREAEEFVTNLAADGRTLLFVGTKRQAQDVIAEEAVRCGMFFINQRWLGGLLT
                     NFTTIQRSLGRLRDLEAMVTDGRYDTLTKKEQARAEKEKRKLLKNLEGIRQMSRLPDA
                     VFVVDTRKEKIAVDEARKLKIPVIGVVDTNCDPDEVDYVVPGNDDALRAIRLFASKIA
                     DAVTAGRGMRESRIAEDEAARAEEA"
     gene            complement(743..1135)
                     /gene="rpsI"
                     /locus_tag="AC16CY_12140"
     CDS             complement(743..1135)
                     /gene="rpsI"
                     /locus_tag="AC16CY_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3354587.1"
                     /translation="MASIEYYGTGRRKTSTARVFLRPGTGAITVNHREFDRYFTTDAL
                     RTVVRRPLLLTETGDKFDILATVGGGGVNGQAGAMRLGIARALVEFNAELRKQLKKDG
                     LLTSDSRVKERKKYGLAGARKRFQFSKR"
     gene            complement(1137..1568)
                     /gene="rplM"
                     /locus_tag="AC16CY_12145"
     CDS             complement(1137..1568)
                     /gene="rplM"
                     /locus_tag="AC16CY_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010547342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR3354588.1"
                     /translation="MRTFIASAKRAEREWHLIDAQGQVLGRIATLTARLLQGKHKAVY
                     TPFIDTGDHVIVINAGTVKLTGQKEKDKIYRRHSGYEGGLREERATDVRKRRPVRLVE
                     EAVRGMLPKTKLGDAMYRKLKVYAGTDHPHKAQQPKRIEVS"
     gene            2043..3113
                     /gene="pilM"
                     /locus_tag="AC16CY_12150"
     CDS             2043..3113
                     /gene="pilM"
                     /locus_tag="AC16CY_12150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HIR3354589.1"
                     /translation="MFGMLGKNKSVVGLDIGSSAVKAVELKPSGKGFRVVAYGAEPVP
                     ADAIVDGAIIDAGAVADSIRNVFEHNRTFKSKDVCASLSGNAVIVKKITLPVMTQSEL
                     DESIYWEAEQYIPFDIQDVNLDYQILDAGTGPDARGNMEVLLVAAKKEKIADYTGVIT
                     QAGRTPVIVDVDAFALQNAYDANYGLDAGQVVVLLNAGASAININILQGEQSMFTRDI
                     SMGGNAYTEAVQKELDLPHESAEQLKKGIPVDGASFEEAQPVLRAVTENVLLEVQKTF
                     DFFKATASSDRIDRIMLSGGASRVDGFRDMLAERFGATVEEFDPFRTIAWDPKSLGRE
                     AVDHASTAAVAVGLALRKVGDR"
     gene            3110..3751
                     /locus_tag="AC16CY_12155"
     CDS             3110..3751
                     /locus_tag="AC16CY_12155"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR3354590.1"
                     /translation="MIRINLLGVERQKARKAPTFDVGQRLTVACSLILVAAVVGIGWW
                     YWALSKNSARVDAEIAAAQQEAARLQAVIAEVLKFEAQRARLQQRVQLIEQLRSGQSI
                     PVQLLDHVSRSLPEMLWLTDMAQDGGAVTIQGKSTTLVALSDFVGALGNGALLQKPIE
                     IVNSVVENAAAGGGQQQAGIDLINFTVRAQLAASGRGAAAGAAGRGASPPPAK"
     gene            3804..4418
                     /locus_tag="AC16CY_12160"
     CDS             3804..4418
                     /locus_tag="AC16CY_12160"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR3354591.1"
                     /translation="MAINLSLSKLPWYSQVGVFVVIALAGAGAFWNFYARDVQESIDA
                     REAQLAKLRSDIDRGLQTARRLPEFRREVGQLEAQLERLRAVLPEEQDVADLLRRVQA
                     MATQSRLTIRGFTPQPVTKRQMHAEWPIGLQLEGTYHDLGMFLERVSKFPRIINVGDI
                     HIKAETRSDGSTISAECTATTFVLLDQQASAAPAGAASKPEQRP"
     gene            4415..4963
                     /locus_tag="AC16CY_12165"
     CDS             4415..4963
                     /locus_tag="AC16CY_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354592.1"
                     /translation="MTRVLTSILAGLLATQAVAAQAPQAGSAPSAPASSGAAPAENTQ
                     KPAEAQKPAEAQKPADTKPDAAVPQGFNYDPQGRRDPFVSMLRRGNDNSGPRLSGLAG
                     LSTSEVSLRGVVRSEGSFVGILQGVDSRNYIVRAGDKLADGTIQRITADTMVILQQIN
                     DPLSLEKQREVRKLLRQVEEAK"
     gene            4984..7287
                     /locus_tag="AC16CY_12170"
     CDS             4984..7287
                     /locus_tag="AC16CY_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR3354593.1"
                     /translation="MSATYRYSMLLGIGLALALGALNPLRAAGGADPAMLKRIASSVD
                     DRTGVIAIEASTPVAYVASQPDPNTFVVELRDVVSAGFQNQFAADPRHPISAVQVENG
                     RASDGAVVARVRMTLEQPMRPRVRSARNVIYVEADRPGNRPASGAPPISLGGPAAAIR
                     DVRVLRRGNATAVTLLGTSRLVASSIAEPKTGPRRVVVTLVNTTSAVPNTTPVGQGPV
                     ERVRIGIDPVQPLLTMVNVDLSRPAPYRVESSPDGNDLTLVFDEPVADPFSALQSTTA
                     SVSREPATASASRESALDTPAPDAAVPPRVVAAPPRVAAAQAPPAAPQTTAAPQPAPQ
                     APAAQAIQPQGPRYTGNPVTLDFQGADLRAVLRTFSEISGLNLVIDPTIQGTVDVALR
                     DVPWDQALDVILRANKLGYVVDGSIVRIAPLTVLADEEAQRRKLSDEQALAGELRVMT
                     RALSYARAEDLRQLLTATVLSQRGSIQFDERTNTIIVNDLPERLERAAALLNNLDRAQ
                     PQVEIEARIVTTTREFARNIGVQWGVGARASTALGNTMPLAFPNQGSITGRTGGIQGP
                     PSPDPQTDSAATVVNMGVSPATSGIGLALGAVNGAVNLDVALTALERSGQGRILSTPR
                     VSTQNNIEAEITQGVQIPIQTVANNTVTVTFRDAALTLRVTPQITATNTVIMRISVEN
                     ASPDFSRAINGIPPIDTQRAITQVLVGDGDTTVIGGIYVSKEQAAQDRTPGLYRIPLL
                     GWLFQRNQLTDDSRELLIFITPRIARL"
     gene            7300..7926
                     /locus_tag="AC16CY_12175"
     CDS             7300..7926
                     /locus_tag="AC16CY_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354594.1"
                     /translation="MTAKPVKFVALAMLVALAAGCGEFTRQGQAPVVVVVERIEAAKG
                     NAPGNLSGTLNSDVVVFRTTPAPCTAAVPCPTIFNDVAEVEMRVRSKDPGVPGLASNP
                     SFLNEVTINRYHVEYIRPDGRNTPGVDVPYGFDSALTFTIPNDGTATAGFEIVRHVAK
                     EEAPLKALAFNGNLIYTIAYITFYGRDQAGNDVSVTANIGINFGDFAD"
BASE COUNT         1370 a         2664 c         2599 g         1309 t
ORIGIN      
        1 gcttcctcgg cgcgcgccgc ctcgtcctcg gcgatgcgcg actcacgcat gccgcgtccc
       61 gccgttacgg cgtcggcgat cttcgacgca aacaggcgga tggcccgcag cgcgtcgtcg
      121 ttgcccggca cgacgtagtc cacttcgtcg ggatcgcagt tggtgtccac cacgccgatg
      181 accgggattt tcagcttgcg cgcctcgtcc acggcgatct tttccttgcg cgtatcgacc
      241 acgaagacgg cgtcgggcag tcgagacatc tgccggatgc cttcgaggtt cttgaggagc
      301 ttgcgcttct ccttctcggc gcgggcctgc tccttcttcg tgagcgtgtc gtaccggccg
      361 tcggtgacca tcgcttcgag atcccggagg cgtccgaggc tgcgctggat cgtggtgaag
      421 ttggtgagca gtccgccgag ccaccgctgg ttgatgaaga acatcccgca gcgcaccgct
      481 tcttcggcga tgacgtcctg ggcctgccgc ttggtgccga caaagagcag cgtacgaccg
      541 tcggcggcca ggttggtcac gaactcctcg gcctcgcgga acttcttgac cgttttgttg
      601 aggttgatga tgtagatgcc gttccgctcg ccgaagatgt actcccgcat cttcgggttc
      661 caccgcttcg tctggtggcc gaaatgcacg ccggcctcca gtagatcctt catcgcaatc
      721 ccggacaaga gcgctccctt ccttagcgtt tgctgaactg gaagcgcttc cgcgcaccag
      781 ccaggccgta cttcttgcgt tccttcacgc gtgaatccga cgtgaggagg ccgtccttct
      841 tcagttgctt ccgcagctcc gcgttgaact cgacgagcgc ccgcgcgatc ccgaggcgca
      901 tggcgccggc ctgtccgttc acgccgccgc cgccgaccgt cgccaggatg tcgaacttgt
      961 cgccggtctc ggtgagcagc agcggacgac ggacgaccgt gcgcagcgcg tcggtcgtga
     1021 agtatcggtc gaactctcga tggttgacag tgattgcccc tgtgccgggc cggaggaaca
     1081 cacgcgcagt ggacgtcttg cggcggccgg ttccgtagta ttcgatgctt gccacgctag
     1141 ctgacctcga ttctcttcgg ctgctgcgcc ttgtgcggat gatcggtgcc cgcgtagacc
     1201 ttcagcttgc ggtacatcgc gtcacccagc ttcgtcttcg gcagcatgcc gcgaaccgct
     1261 tcctccacca gacgcaccgg ccggcgcttc cgcacgtcgg tcgcgcgctc ctcgcgcaac
     1321 ccgccttcgt atccgctgtg ccggcggtag atcttgtcct tttccttctg gcccgtcagc
     1381 ttcacggtgc ccgcgttgat cacgatcacg tggtcgcctg tgtcgatgaa gggcgtgtac
     1441 accgccttgt gcttgccctg gagcagccgc gccgtgaggg tcgcgatgcg tcccagcacc
     1501 tgcccttgag cgtcaatgag atgccactct ctctcggcgc gcttcgcgct cgctatgaac
     1561 gtacgcatga aaacagggag tccttcgcgc ccgccgccat ggcagggaac cgggttggca
     1621 cgctgcccag ggcccgcgcc ctcatcggct ggcgggtccc tctggacgaa cgtggccaac
     1681 gaccgcaaac cctgatcata cggacgctgc ctaaagctgt caagccacag atgcagaggg
     1741 acttcgcggt ctatcgagga gcgcgcgcca aggcgggaat cgctttcgtc aggcctttgt
     1801 atcgtcccta ccaattctgc cgataacaac tttgtgagtc gcgggccgtg catgtcggcc
     1861 gcgacagaag tgtcagtcga agacggtttt gtcgagcgcg gccggagccg ggcgtcgacg
     1921 tgcggtcgat ccgaaccgca gagtttggcc gaaatcggcc gaaggatgga tcgacgggag
     1981 cgtggcatgg cggttgcgcc gctgctcccg gatctcgatc gagatcgaaa agagacctcc
     2041 ttatgtttgg catgcttggg aaaaacaagt cggtcgtcgg gctcgacatc gggtccagcg
     2101 ccgtgaaggc ggtcgagttg aagccgtcgg gcaagggctt tcgcgtcgtg gcatacggcg
     2161 ccgagccggt cccggccgac gccatcgtgg acggcgcgat catcgacgcc ggggcagtcg
     2221 ccgattccat ccggaacgtg ttcgagcaca accgcacctt caagtcgaag gacgtgtgcg
     2281 catcgctctc cggcaacgcc gtgatcgtca agaagatcac gctgccggtc atgacccagt
     2341 cggagctgga cgagtccatc tactgggaag ccgagcagta catcccgttc gacatccagg
     2401 acgtcaacct cgattaccag atcctcgacg cgggcaccgg gcccgacgcg cgcggcaaca
     2461 tggaagtgct gctcgttgcc gccaagaaag aaaagatcgc ggactacacg ggtgtcatca
     2521 cccaggcggg acgcacgccg gtcatcgtcg acgtggacgc cttcgcgctg cagaacgcgt
     2581 acgacgccaa ctacggcctg gacgcgggcc aggtcgtcgt gctgctgaat gccggcgcca
     2641 gcgcaatcaa catcaacatc ctgcaaggcg agcagtcgat gttcactcgc gacatctcga
     2701 tgggcggcaa cgcctacacg gaggccgtgc agaaagagct cgacctgcct cacgagtcgg
     2761 ccgagcagct caagaagggc attcccgtag acggcgcctc tttcgaggaa gcgcaacccg
     2821 tcctccgggc ggtgaccgaa aacgtcctcc tcgaggtgca gaagaccttc gacttcttca
     2881 aggcgaccgc gtcttccgac cggatcgacc ggatcatgct gagcggcggc gcctcgcgtg
     2941 tggacggatt ccgcgacatg ctcgccgagc gcttcggcgc cacggtcgaa gagttcgacc
     3001 cgttccgcac gatcgcatgg gacccgaaga gccttggcag ggaagcggtt gaccacgcat
     3061 ccacggccgc cgtcgccgtc ggcctcgcgt tgcgcaaggt gggggatcga tgattcgcat
     3121 caacctcctc ggcgtcgagc gccagaaggc gcgcaaggcg cccaccttcg acgtcgggca
     3181 gcggctgacc gtcgcctgca gcctgatcct cgtggcggcg gtcgtcggca tcgggtggtg
     3241 gtactgggcc ctcagcaaga actcggcgcg cgtcgatgcc gagatcgcgg ccgcacagca
     3301 ggaggccgcg cggctgcagg ccgtcatcgc cgaggtcctg aagttcgagg cgcaacgcgc
     3361 gcgccttcag cagcgggtcc agctgatcga gcagctccgc tccggccagt ccattcccgt
     3421 gcagctgctc gaccacgtga gccgcagcct gccggagatg ctgtggctga cggacatggc
     3481 gcaggatggc ggcgccgtca ccattcaagg caagagcacg acgctcgtgg cgctgtcgga
     3541 cttcgtaggg gccctgggga acggcgcgct cctgcagaag ccgatcgaga tcgtcaacag
     3601 cgtggtcgag aacgccgccg cgggcggcgg ccagcagcaa gcaggcatcg acctgatcaa
     3661 tttcaccgtg cgcgcgcagt tggccgcgag cggacgaggc gcagcggccg gcgctgccgg
     3721 gcgcggagcg tcaccgcccc cagccaagtg agcggcggcg acatcggtgg gtacaaggcg
     3781 agacggatcg gagtgagggt cgagtggcaa tcaacctgag tctcagcaag ctgccgtggt
     3841 acagccaggt tggcgtcttc gtggtgatcg ccttggcggg cgccggcgct ttctggaatt
     3901 tctacgcgag agacgtgcag gagagcattg acgcgcggga agcgcaactg gcgaagctgc
     3961 gcagcgacat cgaccggggc cttcagaccg cgagacgcct cccggagttc cggcgcgaag
     4021 tcgggcagct cgaggcgcag ctcgaacggc tgcgcgcggt gctccccgaa gagcaggacg
     4081 tggcagacct tctgcgccgc gtgcaggcga tggccacgca gtcgcggttg accatccgcg
     4141 gcttcacgcc tcagccggtc acgaagcgcc agatgcacgc cgagtggccg atcgggcttc
     4201 agctcgaggg cacctaccac gacctcggga tgtttctcga gcgtgtgagc aagttccccc
     4261 gcatcatcaa tgtcggcgac attcacatca aggcggagac acggtccgac ggatcgacga
     4321 tcagcgccga gtgcaccgcg actacgttcg tccttctcga ccagcaggcg tcggccgctc
     4381 cggccggtgc ggcgtcgaag ccggagcagc gcccgtgacc cgcgtcctga cgtcgattct
     4441 tgcgggtctg ctggcgacgc aggccgtggc agcacaggcg ccgcaggccg ggtcggctcc
     4501 gtcggccccg gcgtcttccg gggcagcgcc ggcagagaac acgcagaagc cggcagaggc
     4561 acagaagccc gcagaggcac agaagcccgc cgacacgaag ccggacgccg cggtacctca
     4621 gggattcaac tacgacccac aaggccgccg cgacccgttc gtcagcatgc tccggcgcgg
     4681 aaatgacaac tcaggcccgc gcctctccgg attggcgggg ctgtcgacgt ccgaggtaag
     4741 cctgcgcggg gtggttcgca gcgaggggag cttcgtcggc atcctccagg gcgtggacag
     4801 caggaactac atcgtacgcg cgggcgacaa gctggccgac ggaacgattc agcgcatcac
     4861 agccgacacg atggtcattc tgcagcagat caacgatccg ctttcgctcg agaaacaacg
     4921 ggaagtacgc aagttgctca gacaggtgga agaggccaag tagtggagag gtttgggcag
     4981 aacatgtcag ccacgtaccg atattcgatg ctgctcggga tcggactcgc cctggcgctc
     5041 ggagcgctga acccgctccg cgcggccggc ggcgccgacc cggcaatgct gaagcgaatt
     5101 gcgtccagcg tggacgatcg cacgggagtg attgccatcg aggcgtccac tcccgtggca
     5161 tacgtcgctt ctcagcccga cccgaatacg ttcgtcgtcg aactgcgcga cgtggtgtcg
     5221 gccggcttcc agaatcaatt cgcggccgat ccccgccacc cgatctcggc cgtgcaggtc
     5281 gagaacgggc gagcgagcga cggcgcggtc gtcgcccggg tcaggatgac gctcgagcag
     5341 ccgatgcgcc cgcgggtacg gagcgcccgc aatgtgattt acgtggaggc cgacagaccc
     5401 ggcaatcgcc cggcgtctgg ggcgcccccg atcagcctgg gtggcccggc agcggccatc
     5461 cgtgacgtcc gggttcttcg ccggggcaat gccacggcgg tcacgctgct gggcacatcg
     5521 cgcctcgtgg cgtcgagcat tgccgagccg aagactggcc cgcggcgggt ggtcgtcacc
     5581 ctcgtgaaca cgacgtcggc ggtgccgaac accacaccgg tcgggcaggg gcccgtggag
     5641 cgcgtgcgca tcggcatcga tccggtccag ccgctgctga ccatggtgaa cgtggacctg
     5701 tcgcggcccg ctccgtaccg cgtcgagtcg tctccggacg gcaacgacct gacgctcgtg
     5761 ttcgatgagc cggtcgccga ccccttcagc gccctgcagt ccacgaccgc ttccgtttcc
     5821 cgcgagcccg cgacggcttc ggcttctcgc gagtccgcac tggacacgcc cgccccggac
     5881 gcggccgtgc ccccgcgcgt tgtcgccgcg cccccgcgcg tggcggccgc tcaggcccct
     5941 cccgcggccc cgcaaacgac cgccgctccc cagcccgctc cccaggctcc tgcggcgcag
     6001 gccattcaac cgcagggccc ccggtacacg ggaaacccgg tcaccctcga ctttcaaggc
     6061 gcggacctgc gcgccgtgct gcggacgttc tccgagatca gcgggctgaa cctcgtgatc
     6121 gatccgacga tccagggcac ggtggacgtg gccctccgcg acgtgccgtg ggaccaggcg
     6181 ctcgacgtca ttctgcgcgc caacaaactc gggtacgtcg ttgacggctc aatcgtccgg
     6241 atcgcgccgc ttacggtgct ggccgacgag gaggcgcagc gccggaagct gtccgacgaa
     6301 caggcgctgg ccggcgagct gcgagtcatg acccgggcgc tgagctacgc gcgcgccgag
     6361 gatctgaggc agttgctgac ggcgacggtt ctctcgcagc ggggatcgat tcagttcgac
     6421 gagcggacca atacgatcat cgtcaacgac ctgcctgaac gtctcgagcg agctgcggct
     6481 ctcctcaaca acctggaccg cgcgcagccg caggtcgaga tcgaagcccg cattgtgacg
     6541 accacgcgcg agttcgcccg gaacatcggt gtccagtggg gtgtgggtgc gcgcgcgtcc
     6601 acggctctcg gcaacacgat gccgctcgcg tttcccaacc aggggtcgat tacgggccgc
     6661 accggaggca tccagggccc gccctcgccc gatccgcaaa cggactcggc ggccacggtg
     6721 gtgaacatgg gcgtcagccc ggcgaccagc ggaatcggcc tcgcgctcgg ggcggtcaac
     6781 ggcgcggtga atctcgatgt cgcgctcacg gcgctcgaac ggagcggcca gggccggatc
     6841 ctctcgacgc cccgtgtctc gacgcagaac aacatcgagg ccgagatcac gcagggcgtg
     6901 cagattccaa tccagacggt ggccaacaac accgtgaccg tcacgttccg cgatgcggcg
     6961 ctgacgctgc gcgtgacccc gcagatcacc gcgaccaata ccgtgatcat gcgcatctcg
     7021 gttgaaaacg cctcgcccga tttcagccgc gcgatcaacg gcattccgcc gatcgacacg
     7081 cagcgcgcca tcacgcaagt gctcgtggga gacggggaca cgacggtcat cggcggcatc
     7141 tacgtgagca aggagcaggc ggcccaggac cgcaccccgg ggttgtaccg aatcccgctg
     7201 ctcggctggc tgttccagcg gaaccagttg accgatgaca gccgcgagct gctcatcttc
     7261 atcacgccac ggatcgcccg gctttaggag agaacaccag tgactgcgaa acccgtcaag
     7321 ttcgtcgcgc tcgcgatgct ggtggcactc gccgccggat gcggcgagtt cacacggcaa
     7381 ggtcaagcgc cagtcgtcgt cgtcgtcgag cggatcgagg cggcaaaggg caacgcgccg
     7441 ggcaacctgt ccggcacgct gaactcggac gtcgtggtct tcaggacgac cccggcgcca
     7501 tgcacggcgg ccgtgccgtg cccgacgatc ttcaacgacg tcgccgaagt ggagatgcgt
     7561 gtgagatcca aggaccctgg cgtgccgggg ctggcgtcca acccgagctt cctgaatgag
     7621 gtcacgatca atcgctacca cgtcgagtac atccgtccgg acggccggaa cacgccaggc
     7681 gtggacgtgc cgtacgggtt cgactccgcc ctgacgttca cgatccccaa cgacggcact
     7741 gcaacggccg gtttcgagat cgttcggcac gtcgccaagg aagaggcgcc cctgaaagcg
     7801 ttggccttca atggaaacct catttacacg attgcgtaca tcacctttta cggccgcgat
     7861 caggcaggta acgacgtcag cgtgaccgcc aacatcggca tcaatttcgg cgacttcgcg
     7921 gactaggaca tggctatgca ca
//
LOCUS       DBNGSH010000528         2690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_560053, whole genome shotgun sequence.
ACCESSION   DBNGSH010000528 DBNGSH010000000
VERSION     DBNGSH010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_560053"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC16CY_12180"
     CDS             complement(<1..518)
                     /locus_tag="AC16CY_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354595.1"
                     /translation="MTDAAVGRLLVASLHQSIADLLPMRLEFYESWLNPSGLREGRIG
                     IAPLIAVMSFLRLEGEPYHLITARAGEYTAEWTVADLSRPYKRFVLAMPPAIRKRMVV
                     SVAEWMVQSTYGATSITVRWRQLRGTIALRGSLFCEVRDKVDHPLCEFYAAAFRRLMT
                     LFQLEADVVTEQ"
     gene            782..>2690
                     /gene="argS"
                     /locus_tag="AC16CY_12185"
     CDS             782..>2690
                     /gene="argS"
                     /locus_tag="AC16CY_12185"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR3354596.1"
                     /translation="MILPVHRQLRDHLSRILASLYSLDPASLPPLVLDSPPNRDLGDL
                     GTPAAFELARRLRKAPRAIAQEIAGAFGALQGVRQVTAAPNGYLNFFLERPAFLLQRL
                     GPSAPQARGDAGKAIVEHTAINPNKAAHIGHLRNAALGDTLVRVLRFNSVPVEVQNYI
                     DDTGVQVADVVVGFRVLEGKTLEEVRAIADTTRFDYYCWDLYARVTEWYGQDKARGDV
                     RARTLHDIEHGGNENAAIAAFIADRIVRCHLATMARMNVDYDLLTWEGDILRLKFWAQ
                     AFEVLKAKGAVYLRQDGRLAGCWVMPIQEDIESTPNSQGPTPKAQLPTPNSQPPTPDA
                     QLEASNAAGEDGDDEEREKVIVRSNGVVTYVGKDIAYQFWKLGLLGRDFQYRVFAPRP
                     HGPLWATSSTDGAAEHPAYGGASYVYNVIDVRQSYLQKLLKQALIAVGHPEGAERSHH
                     FSYEMVALSHATARELGFAPPPDSEDAKRPFVEVSGRKGLGVKADDLLDTVIRKAGTE
                     VSKRNPELPEAEVARTAGLIGIAAVRYFLIKFSRGKVIAFDLDEALSFEGESGPYIQY
                     AVVRANNIFQKLQQRDGLSETALLTALKEVPPGELAGENGNHELWGLVLEASRLDEIV
                     EQVIRSLEFSVL"
BASE COUNT          491 a          898 c          872 g          429 t
ORIGIN      
        1 cactgctcgg tcacgacgtc ggcctcgagc tggaacagcg tcatcaggcg gcgaaacgcg
       61 gcggcgtaga actcgcacag cggatgatcg accttgtcgc gtacctcgca gaagagcgag
      121 ccgcgcagcg cgatcgtccc gcgcaactgc cgccagcgca ccgtgatgct ggtggcgccg
      181 taggtgcttt gcaccatcca ctccgccacg ctgacgacca ttcgcttgcg aatggccggc
      241 ggcatggcga ggacgaaccg cttgtacgga cgcgacaggt cggccacagt ccattcggcg
      301 gtgtactccc cggcgcgcgc ggtgatcaga tggtacggct cgccttcgag ccgcaggaag
      361 ctcatgaccg cgatgagggg cgcaatcccg atgcgaccct cgcgcaaccc cgaggggttg
      421 agccacgact cgtagaactc gagccgcatc ggcagcaggt ccgcgatact ctggtgcagg
      481 cttgcgacga gcagacgccc tactgccgca tcggtcatac actaaccccg gagctcgctt
      541 gtgtcggtga tttcggcaag atttgtgtga gcacgagcgg ccgtctcgtc agcgtcgcgc
      601 tggccggagt ccccccaaca caagcgagtt tgcagagtag aatcgaggcc tgtgcgtcga
      661 cgctgcactc gagcgtctga gatagagaga ttgatccgga agcgtggaca ccgtatggcc
      721 ggcggctgaa agggtaccgc acctcgaacg ccaatcgcaa atcgctgacc gtgacacctg
      781 aatgatcctc cccgtccaca gacaactccg cgaccacctc tcgcgcatcc ttgcatcgct
      841 gtactcgctc gatccggcgt cgctgccgcc gctcgtgctc gactctcccc cgaatcgcga
      901 tctgggggac ctgggcacgc cggccgcctt cgagcttgcc cgccggctcc ggaaggcgcc
      961 gcgcgcgatc gcgcaggaga tcgccggcgc cttcggcgcc cttcagggcg ttcgccaggt
     1021 gacggccgcg cccaacgggt atctcaactt cttcctggaa cgtcccgcgt tcctactgca
     1081 gcgcctgggc ccttcggcgc cgcaggcgcg aggcgatgcg ggcaaggcga tcgtcgaaca
     1141 cacggccatc aacccgaaca aggcggccca catcgggcac ctgcgcaacg ccgcgctcgg
     1201 cgacacgctg gtgcgcgtgc tccggttcaa ttccgtgccc gtcgaggtcc agaactacat
     1261 cgacgacacg ggcgtgcagg ttgccgacgt cgtcgttggc ttccgcgtcc tcgagggcaa
     1321 gacgctcgaa gaggttcgtg cgatcgccga caccacgcga ttcgactact actgctggga
     1381 tctctacgcg cgggtgaccg agtggtacgg gcaggacaag gcgcgcgggg acgtcagagc
     1441 acggaccctc cacgacatcg agcacggcgg caatgaaaac gccgcgatcg ccgcgttcat
     1501 cgccgatcgc atcgtccggt gccacctggc gacaatggcg cggatgaacg tggactacga
     1561 cctgctgacg tgggaaggcg acatcctgcg cctgaagttc tgggcccagg cgttcgaggt
     1621 gctcaaggca aaaggggctg tgtatcttcg tcaggacggg cgcctggcgg gctgctgggt
     1681 gatgcccatt caggaagaca tcgagtcaac tcccaactcc caaggcccaa ctcccaaggc
     1741 ccaactcccg acgcccaact cccaaccacc aactcccgac gcccaactcg aggcgtccaa
     1801 cgccgccggc gaggatggcg acgacgagga gcgggagaaa gtgatcgtca ggtccaacgg
     1861 cgtcgtcacc tacgtgggca aggacatcgc ctatcagttc tggaagctcg ggctgctcgg
     1921 gcgggacttt cagtatcgcg tcttcgcgcc ccgcccgcac gggccgttgt gggcgacctc
     1981 cagcacggac ggcgcggcgg agcaccccgc gtacggcggg gcgtcctacg tctacaacgt
     2041 catcgacgtc cgccagtcgt acctgcagaa gctgctgaag caggcgctca tcgcggtggg
     2101 acatccggaa ggcgccgagc gctcgcacca tttctcatac gagatggtgg cgctgtcgca
     2161 tgcaaccgcg cgtgagctcg gcttcgctcc gccgcccgac tcggaagacg ccaagcggcc
     2221 gtttgtcgag gtgtcgggcc ggaaagggct cggcgtcaag gccgacgatc tactggacac
     2281 ggtgatccgg aaggctggca ccgaggtctc caagcggaac cccgagctgc ccgaagccga
     2341 ggtggcgcga acggccggcc tcatcggcat cgcggcggta cgctacttcc tcatcaagtt
     2401 ctcgcggggc aaggtgatcg ccttcgatct ggacgaggcg ctcagcttcg agggtgagag
     2461 cggaccctac attcagtacg cggtcgtacg ggccaacaat atcttccaga aactgcagca
     2521 gcgggatggg ctgagcgaga cggccctgct gacggctctc aaggaggtcc ccccgggcga
     2581 gcttgccggc gagaacggca accacgagct ctgggggctg gtgctcgagg cctctcgact
     2641 ggacgagatc gtcgagcagg tgatacggtc actcgagttc tcggtcctgg
//
LOCUS       DBNGSH010000529         3098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_729880, whole genome shotgun sequence.
ACCESSION   DBNGSH010000529 DBNGSH010000000
VERSION     DBNGSH010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_729880"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC16CY_12190"
     CDS             complement(<1..466)
                     /locus_tag="AC16CY_12190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3354597.1"
                     /translation="MLAQTVAELYEAYGPRMYRYAVLLLADRAAAEDAIQEVFLQIAR
                     ASDRSGFDVTFAYLATTVRNTCYSALRRRRRRNEVGDPVLERASPDATEEERMMIDQA
                     LKTLPAEQREVVYLKVFEGLTFQEIADRCDVSINTAGSRYRYAMQSLRRALSP"
     gene            complement(469..900)
                     /locus_tag="AC16CY_12195"
     CDS             complement(469..900)
                     /locus_tag="AC16CY_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354598.1"
                     /translation="MRKRQAAFVVLYLAFAAGTADAQECLHGPTETAEQAARRFQALA
                     ATRAVNTMQANEAGLRGGLYMRHEELANSAAASRMRESTNATMKRISLIPGTDILPDW
                     ELTLDVTERGYWFMIKDKVDPCGFAFVSNQVGLIFNAEPIR"
     gene            1064..1315
                     /locus_tag="AC16CY_12200"
     CDS             1064..1315
                     /locus_tag="AC16CY_12200"
                     /inference="COORDINATES: protein motif:HMM:NF022603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HIR3354599.1"
                     /translation="MPRAPRPPAPTPVSLPQPKLIRKTRNAPSRAPAAVPHELIALHA
                     YWLFLERGCVHGHDLDDWFMAERELLESQAAPVRKAASA"
     gene            complement(1392..2654)
                     /locus_tag="AC16CY_12205"
     CDS             complement(1392..2654)
                     /locus_tag="AC16CY_12205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR3354600.1"
                     /translation="MLLTLDIEKATAGGRMLARHQGQVVLVSGAIPGERVSARLERTG
                     KGVAFAEVVEVLSPSADRRSVAADARCGGNVLAHVNYARQLRLKGEIIQDALGRIGRM
                     PIPAPPDVIGSPERGYRMRARFHVQGWRIGYYLEGTHRLCDAAATGQLLAATADWIAA
                     AERTLASARFTGVSAIELAENVAGDERACHLELVAGADPRPLAALSEGLSGLSAQRAD
                     RADVVRIAGTPAVTDVVRVSHVDAPIVRLRRDVRSFFQGNRFLLEPLVRCVAGLVEQG
                     PVIDLYAGVGLFGLSLAAAGRDPVTLVEADPVSGADLQHNAEPFAPRVTVARRKVETF
                     LAEARRSDAADATVIVDPPRTGLSKEAAADIIRLSPPQIVYVSCDVATFARDTRTLAD
                     AGYRLDSLKGIDLFPNTAHVETIASFTR"
     gene            complement(2772..>3098)
                     /locus_tag="AC16CY_12210"
     CDS             complement(2772..>3098)
                     /locus_tag="AC16CY_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354601.1"
                     /translation="RAGADRTLDDVLDAIVRELDAARASAGRLRGDARQALLARLAQL
                     DVELVAAARNRCDPSALAALGAEADAELAPFRSRMSSELFEQSHRAAVDRLIRERDRL
                     PTLTFD"
BASE COUNT          527 a         1063 c          989 g          519 t
ORIGIN      
        1 caggactcaa ggctcgtcga agcgactgca tggcgtaccg ataccggctg cccgcagtgt
       61 tgatgctgac gtcgcaccgg tccgcgatct cctgaaacgt aagtccttcg aagaccttca
      121 gatacaccac ctcacgctgc tccgccggca gcgtcttgag cgcctgatcg atcatcatcc
      181 gctcttcctc ggtggcgtcc ggcgacgccc gttcgaggac ggggtccccg acctcattgc
      241 gccggcgccg tcgtcgaaga gcgctgtagc acgtgttccg aaccgtcgtg gcaaggtacg
      301 caaacgtgac gtcgaacccg ctacgatcgg aggcccgagc gatctgcaga aacacctcct
      361 gaatggcgtc ctcggcggcc gcccggtcgg ccagtaacag caccgcatat cggtacatcc
      421 gcgggccgta ggcctcgtac agctcggcga cggtttgtgc aagcaaccct agcgaatggg
      481 ttcggcgttg aagatcagac ccacctggtt gctcacgaac gcaaacccgc acggatcgac
      541 cttgtctttg atcatgaacc agtaaccccg ttcggtgacg tcgagtgtca gctcccagtc
      601 cggcaggatg tccgtgccgg gaatcagcga aatgcgcttc atcgtcgcgt tcgtcgactc
      661 acgcattctg gatgcggcgg ccgagttagc gagttcctcg tgacgcatgt agagcccgcc
      721 gcgcaatcca gcctcattgg cctgcatggt gttgacggcc ctcgtcgctg ccagcgcttg
      781 aaaccgtctc gcagcctgtt ccgcggtctc cgttggacca tgcagacatt cctgcgcgtc
      841 agccgttccg gcggcgaacg caaggtacag aactacgaat gccgcctgcc gctttcgcat
      901 cgccttcctc cttgcagcac ctgatgtgtc gtctgactcc agtcgagtct ctgcggcttg
      961 tcgactaaag aacgctcgag gccccctttc ttgtctgctt agtgaaaaaa gccgcccgtg
     1021 ttgctcgaaa cgcgcgatga ttaggtcaac gggaggtgtt tgcatgccac gtgctcccag
     1081 gccaccggcg ccaacgccgg tcagtctccc ccaacccaag ctgattcgta aaacacgaaa
     1141 tgccccctcg agggcgccgg cggcggttcc gcacgagctg atcgcacttc atgcctattg
     1201 gctgttcctc gaacggggct gcgtgcatgg acacgacctc gacgactggt tcatggccga
     1261 gcgcgaactg ctcgagtcgc aggccgcccc cgtcaggaaa gcggccagcg cctgagcgtt
     1321 tgacgggact cgggctcttt cgcgtccgtg gaggcttgcc ggcgagtccg cgaattctca
     1381 gacgttcttc gtcagcgcgt aaacgacgcg atcgtctcga cgtgcgccgt attcgggaaa
     1441 aggtcgatcc ctttcagcga gtcgagccga taacccgcgt ccgcgagcgt gcgcgtgtcg
     1501 cgcgcgaacg tggccacgtc gcacgacacg tagacgatct gcggcggact cagcctgatg
     1561 atgtccgcgg ctgcctcttt cgagagaccc gtgcgcggcg ggtcgacgat gaccgtggcg
     1621 tccgcggcgt cggaacgccg cgcctcggcc aggaacgtct ccaccttgcg gcgtgcgacg
     1681 gtgacgcgcg gagcgaacgg ctcggcgttg tgctggagat cggcgccgct gacgggatcc
     1741 gcttcaacga gcgtcaccgg atcccttcct gccgcggcca gcgacagccc gaacaggccg
     1801 actccggcat aaaggtcgat caccggcccc tgctcgacga gcccggccac gcagcggacc
     1861 agcggttcga gcaggaagcg attgccctga aagaacgacc tgacatcgcg gcgcagccgg
     1921 acaatcggcg cgtccacgtg gctgacgcgg acaacgtcag tcacggccgg tgttccggca
     1981 atgcgcacga cgtcggcgcg atcggcgcgc tgcgcggata ggccggagag cccctccgac
     2041 aacgcggcga gcggtcgagg atcggcgcct gccacgagct ccagatgaca cgcgcgctcg
     2101 tcgcctgcca cgttctcggc aagctcgatc gcgctgacgc cggtgaaccg tgccgaggcg
     2161 agcgtacgct cggccgccgc aatccagtcg gccgttgccg cgagcagctg gccggtcgcg
     2221 gccgcgtcgc acaaccggtg agtgccctcg aggtaataac cgatgcgcca gccctgcacg
     2281 tgaaagcggg cgcgcatgcg atacccgcgc tcgggcgagc cgatgacgtc gggcggcgcc
     2341 ggaatcggca tccgcccgat gcggcccagc gcgtcctgga tgatctcgcc cttgagccga
     2401 agctgccgcg cgtagttgac gtgcgcgagg acgttgcctc cgcaacgcgc atcggccgcc
     2461 acggaccggc ggtcagccga gggcgacagc acctcgacga cctcggcgaa ggcaacgccc
     2521 ttgcccgtgc gctcgaggcg ggccgagacc cgctctccgg ggatcgcgcc cgagacgagc
     2581 acgacctgcc cctgatgacg cgcgagcatg cggccgccgg cggtcgcctt ctcgatgtcg
     2641 agcgtcagaa gcatgtcacc ggttcttcgt ccgacacacc gaggcaccga gacaccgagg
     2701 cgatcgtcgt atttgaactc ggtgcctcaa tgcctcggtg tgtcgtgatc gtcgatgtaa
     2761 gcgaatcacg ttcaatcgaa cgtcagcgtc ggcagtctgt cacgctcgcg gatcaggcga
     2821 tcgacagccg cgcggtgcga ctgctcgaac agctcggagg acatacggct tctgaacggc
     2881 gccagctcgg cgtccgcctc ggcgccgagc gcggccagcg ccgacgggtc gcagcggttt
     2941 cgagcggccg ccaccaactc gacgtcgagt tgagcgaggc gagccagaag cgcctgccgc
     3001 gcgtccccgc gaagccgccc tgcgctcgcg cgcgccgcgt ccagttctct cacgatcgcg
     3061 tcgagcacgt cgtccagggt tcgatcggcg ccggcgcg
//
LOCUS       DBNGSH010000530         1912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3276828, whole genome shotgun sequence.
ACCESSION   DBNGSH010000530 DBNGSH010000000
VERSION     DBNGSH010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3276828"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="AC16CY_12215"
     CDS             complement(<1..1437)
                     /locus_tag="AC16CY_12215"
                     /inference="COORDINATES: protein motif:HMM:NF019255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HIR3354602.1"
                     /translation="MRRIGVLCVALWILAAASSSLRADPAQSTATGPAATLSADQALV
                     QKYCVTCHNPRARTGGLSLEGMNPADAASHAEVWEKAAMKLRGGMMPPQGMPRPDEAA
                     LRSFAASLEKTIDERALRSPDPGHKPVHRLNRTEYGNAVRDLLDLRVDVTGLLPADDE
                     SHGFDNIAGVLRVSPSLLEQYLASARTISSLAVGTDKELVRSTYRLPPDDSQEDHVEG
                     LPLGTRGGLAFRHNFPQDGDYEFSIKLLRNIVGYMVGLEFGHLVEVTVDGERVFATQV
                     GGEEDNLASDRNMSETADKIDERLKARVPVKAGPRMVGVAFVRRNRAESDEPLQPHER
                     NHDLQDMNGIPLIDFVSVTGPTNPTGPGDTPSRRRIFTCRPSKAADEGGCARAILTNL
                     ARRAYRRPVTSADMNPILERYADGRTKGTFDQGIEQGLRLILASPKFLFRTEDAPAGA
                     AAQRVTDLELATRLSFFLWSSIPDDELLD"
     gene            1524..>1912
                     /locus_tag="AC16CY_12220"
     CDS             1524..>1912
                     /locus_tag="AC16CY_12220"
                     /inference="COORDINATES: protein motif:HMM:NF048868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vgb family protein"
                     /protein_id="HIR3354603.1"
                     /translation="MTVKWLCALGILICTGLAAMAQQRWLEPGQRLTTDGVPTEFPLP
                     AAMSGPTTLALAPDGTVWFTEGAGNRIGRMNPDGSGLKEFPLPNPGSSPRIIALGADG
                     NMWFSEHSGNRIGRITPDGAISEFPIPT"
BASE COUNT          299 a          661 c          625 g          327 t
ORIGIN      
        1 atcgagcagc tcgtcgtcgg ggatcgagct ccacaggaag aacgacagcc gcgtggccag
       61 ctccaggtcg gtcacgcgct gcgccgctgc ccccgccggt gcgtcctcgg tacggaagag
      121 gaacttcgga ctcgccagga tcaggcgcag gccctgctcg atgccctggt cgaaggtccc
      181 cttcgtccgg ccgtcggcgt accgctccag gatcggattc atgtcggcgc tcgtcaccgg
      241 ccggcggtac gcgcgacgcg cgaggttcgt gagaatcgcc cgggcgcatc cgccctcatc
      301 ggccgccttc gaggggcggc acgtgaagat tcgacgccgg ctcggcgtgt cgccggggcc
      361 ggtcggattg gtcgggcccg tcacgctgac gaagtcgatg agcggaatcc cgttcatgtc
      421 ctgcagatcg tgattgcgct cgtgcggctg gagcggctcg tccgactcgg cgcggttgcg
      481 ccgcacgaac gccacgccca ccatccgcgg cccggccttg accggcactc gcgccttgag
      541 ccgctcgtcg atcttgtcgg ccgtctccga catgttgcgg tctgaggcca ggttgtcctc
      601 ctcgcccccg acctgcgtcg cgaacacgcg ctctccgtcg accgtcacct cgaccaggtg
      661 tccgaactcc agtcccacca tgtagccgac gatgttgcgg agcagcttga tgctgaactc
      721 gtaatcgccg tcctgcggga agttgtgcct gaaggcaagg cctccgcgcg tgccgagcgg
      781 cagcccctcg acgtgatctt cctgtgagtc gtccggcggc agccgatagg tgcttcggac
      841 gagttccttg tcggtaccga cggcaagact gctgatcgta cgggcggacg cgaggtactg
      901 ctccagcagg gaaggcgaca cgcgcagcac gcccgcgatg ttgtcgaaac cgtggctctc
      961 gtcgtcggcg ggcagcaggc cggtgacgtc cacccggaga tcgagcaggt cgcggacggc
     1021 gtttccgtac tcggtgcgat tcagcctgtg gaccggcttg tgccccggat ccggagagcg
     1081 cagcgcgcgc tcgtcgatcg tcttctccag cgacgcggca aacgatcgaa gggccgcttc
     1141 gtcgggacga ggcatccctt gaggcggcat cattccgccg cgaagcttca tcgccgcctt
     1201 ttcccacacc tcggcgtgcg aggcggcatc ggccggattc atcccctcga gcgacagccc
     1261 tccggtcctg gctcgcgggt tgtggcaggt cacgcagtac ttctggacga gtgcctgatc
     1321 ggctgagagc gtcgcggccg ggcccgtcgc cgtggactgc gcgggatccg cccgcaggct
     1381 gctggaggcc gcagccagaa tccacaaggc gacgcacaga accccgatcc ttcgcacgct
     1441 cgacacactc ctacctacgc cggaacggca tgggctataa catacgcacc cgcgctcgat
     1501 tcgcggggcg acgagacttt attatgaccg tcaagtggct gtgcgcgcta gggatactga
     1561 tttgcacagg cctcgcggcg atggctcaac agcggtggct ggaaccgggt cagagactca
     1621 ccacggacgg ggttccgacg gagtttccgc tgccggccgc gatgagcggt cccaccactc
     1681 ttgcgctcgc gcccgacggc actgtgtggt tcaccgaagg ggccggcaac cgcatcggac
     1741 gcatgaaccc cgatggctcg ggcctcaagg aattcccgct tcccaacccc ggcagctccc
     1801 cgcgaatcat cgcgctcggc gctgatggca acatgtggtt ttcggagcac agcggcaacc
     1861 gcatcggacg gatcacgccc gacggcgcca tctctgagtt cccgatcccg ac
//
LOCUS       DBNGSH010000531         2274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3276912, whole genome shotgun sequence.
ACCESSION   DBNGSH010000531 DBNGSH010000000
VERSION     DBNGSH010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3276912"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC16CY_12225"
     CDS             complement(<1..217)
                     /locus_tag="AC16CY_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354604.1"
                     /translation="MTRVLPGILLLGLCASSVSCHRGPRPAIPGAAALTPVVSNQVDQ
                     VNMPPWEGGWSIIGRGSPVVRFSQTFTP"
     gene            complement(265..765)
                     /locus_tag="AC16CY_12230"
     CDS             complement(265..765)
                     /locus_tag="AC16CY_12230"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3354605.1"
                     /translation="MKTLAALTFLVTLAACTQAPAPVADTREADAAAIRATEEAWKAA
                     WSTKVADNITPYWAENATLMAPGLPAVKGSAAIKPVIAELVADPNFSLSLQTVTVEAS
                     RGGDFGYTQGTYTVTATDPATKKAVTEKGKYLTLYMKQPDGSWKSVQDIWNADGPATP
                     VEAVGK"
     gene            885..2078
                     /locus_tag="AC16CY_12235"
     CDS             885..2078
                     /locus_tag="AC16CY_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354606.1"
                     /translation="MSTTRMTVVAVALLASMAIGVAGDQVIAQGQGGGRGRGGGPPTS
                     TTPPTGVTPLPADMFTTKNFYLDRKSWIDKRYARCNTPRQITDMWTRSNRVAHWGDCS
                     VDYPVEKIVSPYPYKTAAEHYDALMAEAKKAGGPTRHTRQTLPDWDGWYQRGAAQDQW
                     TYGSNLQTSTMVSLLTPEYQKRMIQTAYHEGVSNSPQWMASFCYPEGFIRWWAQASHG
                     GPIEVMVTPHQVQFLTGIADNLLRRVLIGRTHVQKVPQWYGETVGFWNGNTLVAWTAN
                     VQGWTLSHAMFEISNSMETIEVFRPAPDGRTIEVETTFYDPEAFVRPLRQVTPWTRQR
                     TLDDAEQRFTFVECRVQSTIVNGSDGRPTQLTPVDDGYIDYFGRPWALNWEKHFEQGW
                     EKPEN"
BASE COUNT          418 a          719 c          772 g          365 t
ORIGIN      
        1 tcggcgtgaa cgtctgcgag aacctgacga ccggcgatcc acgtccgatg atgctccatc
       61 cgccctccca gggcggcatg ttgacctggt cgacctgatt gctgacgacg ggggtgagcg
      121 cggccgctcc agggatcgcg ggccgtgggc cccgatggca actgacactg gaggcgcaca
      181 agccgaggag cagaataccg ggcagaacac gcgtcatgta tcgttacgtc gacgctgact
      241 tcagccgctg gccggagcga cgttctattt tccgacggct tccacgggcg tcgccggacc
      301 gtcggcgttc cagatgtcct gcacggactt ccaggatccg tcaggctgct tcatgtacag
      361 ggtcaggtac ttgcctttct cggtgacggc cttcttggtg gcgggatcgg ttgctgtcac
      421 cgtgtaagtg ccctgggtgt atccgaagtc gcctcccctg gacgcttcca cggtcacggt
      481 ctgcaggctg agcgaaaaat tcggatcggc gacgagctcg gcgatcaccg gcttgatggc
      541 ggcggagccc ttcactgccg gcaggccggg cgccatgaga gtggcgttct cggcccagta
      601 gggcgtgatg ttgtcggcga ccttcgtgga ccacgcggcc ttccaggcct cctcagtcgc
      661 gcggatggcc gcggcgtcgg cctcgcgtgt gtcggcgacc ggcgccggcg cctgcgtgca
      721 ggcggccaga gtcacgagga atgtgagagc agcgagtgtt ttcatggcgc cgatcgtatc
      781 tcgaggcgca agccgcaagt gccgcctctg aagcgtggta gcgtattcgg tagccgcgac
      841 gcgcgttcga ccgcgctcgc tcgcgagacg aggagtcagc attcatgagt acgacgcgca
      901 tgaccgttgt cgcggtggca ctgctcgcct cgatggccat cggcgtagcc ggagaccagg
      961 tcatcgccca ggggcaaggc ggcggccggg gccgcggcgg tgggcccccg acgtcaacga
     1021 cgcctcccac gggagtcacg ccgctgccgg ctgacatgtt cacaaccaag aacttttatc
     1081 tcgatcgcaa gagctggatc gacaagcgct acgccaggtg caatacgccg cgccagatca
     1141 ccgacatgtg gacgcgctcc aaccgtgtgg cccactgggg cgactgcagc gtggactacc
     1201 cggtcgagaa gatcgtcagc ccctacccgt acaagacggc ggctgagcac tacgacgcgc
     1261 tcatggccga agccaagaag gcaggcggcc cgaccaggca cacgcggcag acgctcccgg
     1321 actgggacgg gtggtaccag cgaggcgcgg cccaggatca atggacctac gggagcaacc
     1381 tgcagacgtc gacgatggtg tcgctgctca cccccgagta ccagaagcgc atgatccaga
     1441 cggcgtatca cgagggcgtc agcaactcgc cccagtggat ggcctcgttc tgctatccgg
     1501 aaggcttcat ccggtggtgg gcccaggcgt cgcacggcgg gccgatcgag gtgatggtga
     1561 cgccgcatca ggtgcagttc ctgaccggca tcgccgacaa cctgctgcgc cgcgtcctca
     1621 tcggccgcac acacgtgcag aaggtgccgc agtggtacgg tgaaacggtc ggcttctgga
     1681 acggcaacac gctggtcgcc tggacggcca acgtccaggg atggacgctc tcgcacgcga
     1741 tgttcgagat cagcaacagc atggagacga tcgaggtctt ccgcccggct cccgacggga
     1801 ggacgattga ggtcgaaacg acgttctacg accctgaagc gttcgtccgg ccgctgcggc
     1861 aggtcacgcc gtggacgcgc cagcggacat tggacgacgc ggagcagcga ttcacgttcg
     1921 tggagtgccg cgtgcagagc acgatcgtca acggatccga tggcaggccc acacagctga
     1981 cgccggtgga cgacgggtac atcgactact tcgggcggcc ctgggcgttg aactgggaga
     2041 agcatttcga gcagggctgg gagaagccgg agaactgacc gtcatgagac acggagcgac
     2101 tcgaatgacc acacgcacac ggcgcgcatg gatgagggca ggactcgcaa ccagtctcgt
     2161 gctgggcgtc gctgccgccg cggccgcgca tcactccttc gcgatgtaca acatgacgga
     2221 gacgaagacg ctgacgggca agctcacgcg gttcatcccg gggagcaacc acgc
//
LOCUS       DBNGSH010000532         6094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3107596, whole genome shotgun sequence.
ACCESSION   DBNGSH010000532 DBNGSH010000000
VERSION     DBNGSH010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3107596"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="AC16CY_12240"
     CDS             <1..620
                     /locus_tag="AC16CY_12240"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF013811.7"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR3354607.1"
                     /translation="GRVIARPFVGAAGSFLRTSNRHDYALTPPGDTLLDLIARRGDPV
                     VSIGKGRDLFAGRGLTRTGHTASDQEGIAALRDAMDIEPRGLIVANLVDFDTVFGHRN
                     DVAGYAANLERFDAGLPALLERLRGDDLLAITADHGNDPTTPSTDHSREHVPLLLTGS
                     RVRPGANLGTRRTFADLGQTLATNFGVGPLANGTSFLEDMRVEHP"
     gene            604..1662
                     /locus_tag="AC16CY_12245"
     CDS             604..1662
                     /locus_tag="AC16CY_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR3354608.1"
                     /translation="MSSIREALEQRERETLAPQAAKSSQSRGRLKSEPEDDVRPAFQH
                     DRDRIIHSKAFRRLKHKTQVFFAPEGDHYRTRLTHTLEVSQIARTIAKVLRLHEELTE
                     SVALGHDLGHTPFGHAGERVLDSLVPGGFRHYEQSLRVVDVLENDGRGLNLTWEVRDG
                     IGRHSKGREGSPVGLDPSKRAATLEGQIMRVADLIAYVNHDIDDAVRAGVLQDADLPA
                     DAVAVLGRTSSARIARMVKDVVMETQAGGLAEIRMSEQVLSATLRMRAFLFEAVYENE
                     AATAEFKKATGILGGLWERVRERPAGLLDERIIEADGLDVAARDFLAGMSDRFAVSLF
                     ERLFIPKPWVGPMAWNQL"
     gene            1714..2226
                     /locus_tag="AC16CY_12250"
     CDS             1714..2226
                     /locus_tag="AC16CY_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR3354609.1"
                     /translation="MQLDLTRYRQPVSHFGRTFEPVEVGQDDDAYRVVAPVQVDLDIH
                     KDKDRFRLAGRAQTVLELACSRCLEPFRMPFDGSFDLRYLPASELANEDEREVAEEDL
                     DTSYYRDDQIDLNELLREQFYLALPMKPLCQDGCVGLCPQCGRNLNTGTCQCTTQWED
                     PRLAPLKELK"
     gene            2263..2445
                     /gene="rpmF"
                     /locus_tag="AC16CY_12255"
     CDS             2263..2445
                     /gene="rpmF"
                     /locus_tag="AC16CY_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR3354610.1"
                     /translation="MPNPKRRHSKTRTAKRRTHDALRAATTGLCPQCQEPKAPHRICP
                     HCGYYNSRPVRAVEEE"
     gene            2473..3420
                     /gene="fabD"
                     /locus_tag="AC16CY_12260"
     CDS             2473..3420
                     /gene="fabD"
                     /locus_tag="AC16CY_12260"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3354611.1"
                     /translation="MIAFIFPGQGSQKVGMGKALVEAYPEARAAFEEADAAFADAAHP
                     SPALSELCFQGPEDRLTLTEIQQPAILTVSIAAYRVLETRGVRPSLVAGHSLGEYSAH
                     VSAGTFPLGDAVRVVRRRGRFMQEAVPVGEGAMAAILGASLDVVRQACEESAENEVVS
                     PANINGQGQVVIAGHARAVQRASARAKALGARRVIPLSVSAPFHCALMKPAEVRLEPE
                     LRALTVQKPRMPIVANIDGTPKRDAAPAIDALVKQVSAPVQWEAVVHCLASEGVTTYV
                     EVGPGNVLGGMVKKIHADARIVRFGSPDDLDSVMAACSN"
     gene            3408..4148
                     /gene="fabG"
                     /locus_tag="AC16CY_12265"
     CDS             3408..4148
                     /gene="fabG"
                     /locus_tag="AC16CY_12265"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR3354612.1"
                     /translation="MFELGEKVIVVTGASRGIGRGIAEALASRGAHVVAAARGDHASP
                     VVEGIRQAGGHAEGATVDVTDGASVEALVAGVIDRHGRVDGLVNNAGIARDQLMLRMK
                     REEWDEVIATNLTAAFTCVHAVLKPMIKRRAGRIISISSVVGQMGNAGQANYAASKAG
                     LIGFSKALAREVASRNITVNVVAPGLVDTDMTRAVSDKAQAEWTSLIPLQRLGTPQDI
                     AAAVCFLASDEAAYITGQVLNVNGGMYM"
     gene            4241..4480
                     /gene="acpP"
                     /locus_tag="AC16CY_12270"
     CDS             4241..4480
                     /gene="acpP"
                     /locus_tag="AC16CY_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3354613.1"
                     /translation="MAVSDKVKGIIVEQLGVDEEEVTPDASFVDDLGADSLDTVELVM
                     AFEEEFGIEIPDEDAEKITRVKEAIDYIEAHGKKK"
     gene            4492..5733
                     /gene="fabF"
                     /locus_tag="AC16CY_12275"
     CDS             4492..5733
                     /gene="fabF"
                     /locus_tag="AC16CY_12275"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3354614.1"
                     /translation="MSRRVVITGIGLVSSLGIGTDANWKALVAGTSGVDRISKFDVTG
                     FAAQIAAEVRGFDPLDYIDKKDVKKMDVFIQYAVAASQFAMDDSRLEITPSNAPDIGV
                     FIGSGIGGFQTIEREHSALLNGGPRKISPFFIPSAIINLAAGQVSIRFGAKGPNLATC
                     TACSASAHAIGESFEIIRRGDAEAMIAGGSEAAITPMSVGGFGALRALSTRNDNPRGA
                     SRPFDKDRDGFIIGEGAGILILEELDFARKRGARVYAEVVGYGMSGDAYHITAPSEDG
                     DGGVRVMRKAVCKAGIPPDEVDYINAHGTSTPYNDRLETLAIKKLFGDHAFKMAVSST
                     KSMTGHLLGGAGGLEAGISALAVHHQVAPPTINLDDPDPECDLDYVPHKGRPMSIRYA
                     LSNSFGFGGTNAALLFKKFEG"
     gene            5771..>6094
                     /locus_tag="AC16CY_12280"
     CDS             5771..>6094
                     /locus_tag="AC16CY_12280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3354615.1"
                     /translation="MKIAVCIKQVPTREWQPRLREDKAWIREQDASFELNEPDAFALE
                     EALRLREKQGGEVVACAAGPARVQQVIREALARGADRALHVDDEALASADASITSAAL
                     ASAMAD"
BASE COUNT         1073 a         1925 c         2121 g          975 t
ORIGIN      
        1 tagggcgcgt catcgcgcgt cccttcgttg gagcagccgg atcctttctg cggacgtcca
       61 accgccacga ctacgcgctg acgccccccg gtgacaccct gctcgatttg atcgcccgac
      121 ggggggaccc ggtcgtgtcc atcggaaaag ggcgggacct ctttgctggt cgcgggctca
      181 cgcgtaccgg acacacggcc agcgaccagg aggggatcgc cgccctgcgc gacgcgatgg
      241 acatcgagcc ccggggactc atcgtcgcaa acctggtgga cttcgatacg gtgttcggcc
      301 accggaacga cgtggcgggg tatgccgcca acctcgagcg cttcgatgcg ggcctcccgg
      361 ctttgctcga acggctccgc ggcgacgacc tgcttgcgat tacggccgac cacggcaatg
      421 atccgacgac accgagcacc gatcattcgc gagaacacgt gccgctgctg ctgaccgggt
      481 cgcgcgtgcg ccccggcgct aatctgggga cgcgccggac cttcgcggat ctcgggcaga
      541 cgcttgccac caacttcggc gtgggtccgt tggcaaacgg caccagcttc ctggaggaca
      601 tgcgtgtcga gcatccgtga ggccctcgag cagcgcgagc gcgagacgct cgcgccgcag
      661 gcggcgaaga gctcccagtc gcgcggccgt ctcaagagcg agccggagga cgatgtcagg
      721 ccggcgtttc agcacgaccg cgaccggatc attcacagca aggcgttccg ccggctgaaa
      781 cacaaaacgc aggtcttctt tgcccccgag ggcgaccatt accgtacgcg gctcacgcac
      841 acgctcgagg tctcgcaaat cgcccgcacg atcgccaagg tgctccgtct tcacgaggag
      901 ctgaccgagt cggtcgcgct cggccacgac ctgggccata cgccgttcgg gcacgcaggc
      961 gagcgcgtcc tcgattccct cgtgccgggc ggattccgcc actacgagca gagcctgcgg
     1021 gtcgtggacg tcctcgagaa cgacggccgg ggtctcaacc tcacgtggga ggtccgcgac
     1081 ggcatcggcc ggcattcgaa ggggagggaa ggatcgccgg tcggactcga tccctcgaag
     1141 cgggcggcca ctctcgaggg gcagatcatg cgtgtggccg atctcatcgc ctacgtgaac
     1201 catgacatcg acgatgccgt gcgtgccggc gtgctgcagg acgccgacct tccggcagat
     1261 gcggtcgccg tccttggaag gacctcgtcc gcccgaatcg cacggatggt caaggacgtg
     1321 gtcatggaaa cgcaggccgg cggccttgcc gagatccgca tgagcgagca ggtgctgtcc
     1381 gctacgctgc gcatgcgggc gtttctcttc gaggctgtct acgaaaacga ggctgcgacc
     1441 gcggagttca agaaagccac gggaatcctt ggagggctgt gggagcgcgt ccgggagcgt
     1501 cccgcgggcc ttctcgacga gcggatcatc gaggccgatg ggctcgacgt cgccgcccgc
     1561 gattttctcg ccggcatgag cgatcgcttc gccgtctcgc tgttcgagcg cttgttcatc
     1621 cccaagccgt gggtcgggcc catggcctgg aatcagctct aagctataat tgcaggtcgc
     1681 ctttcgcgga aatttcgcgg gaggggggcg gccatgcagt tggacctcac ccggtatcgc
     1741 cagcctgtca gccacttcgg gcgcacgttc gagcccgtcg aggtcgggca ggacgacgat
     1801 gcataccggg tcgtggcgcc ggttcaagtg gacctcgaca tccacaagga caaggacagg
     1861 tttcgtctcg ctggacgcgc gcagaccgtg ctcgagctcg cgtgcagccg gtgcctggaa
     1921 ccgttccgga tgccctttga cggatcgttc gacttgaggt acctgccggc gtcggagctc
     1981 gccaacgaag acgagcgcga agtggccgag gaagatctcg acacgagcta ctaccgggac
     2041 gatcagatcg acctgaacga gttgcttcgc gagcagttct acctggcgct tccgatgaag
     2101 ccgttgtgcc aggacgggtg tgtcgggctg tgcccgcagt gcggcaggaa cctgaacaca
     2161 gggacgtgcc agtgcacgac gcagtgggag gatccgcgcc tcgcgccgct caaggagttg
     2221 aagtaaccgg agaccgtaga ccgtagactg aagaccgaga tcatgccaaa tccgaaacgc
     2281 cgacactcca agacgcgcac cgcgaagagg cgcacgcacg acgccctgcg tgccgcgacg
     2341 acggggctgt gcccgcagtg ccaggagccg aaggcgccgc atcgcatctg ccctcattgc
     2401 ggctactaca actcgcggcc ggtcagggcc gtcgaggaag aatagcgctc agacgtcctc
     2461 tcgacgcgcg acgtgattgc tttcatcttc cccggacagg ggtcccagaa ggttggaatg
     2521 gggaaagcgc tcgtcgaggc gtatccggag gcgcgagcgg ccttcgagga ggccgacgcg
     2581 gcgtttgccg acgccgcgca cccgtcgccc gcgctgagcg agctgtgttt tcagggccca
     2641 gaggaccgtc ttaccctcac cgagattcag cagccggcga tcctgacggt cagcatcgcc
     2701 gcgtaccgcg tgctcgagac gaggggcgtg cggccgtcgc tcgtggccgg ccacagcctg
     2761 ggtgagtact cggcccatgt gtcggccggc acgttcccgc ttggcgacgc cgtgcgcgtg
     2821 gtccggaggc gcggccgatt catgcaggag gccgtgccgg ttggtgaggg cgccatggcg
     2881 gcgatcctcg gcgccagcct cgacgtggtg cgtcaggcct gtgaggagtc cgcggagaac
     2941 gaggtcgtca gccccgcgaa catcaacggt cagggccagg tcgtcattgc cgggcacgcc
     3001 cgtgccgtgc agcgcgcgtc ggctcgcgcc aaggcgctcg gcgccaggcg ggtgattccg
     3061 ctgtcggtca gcgcgccgtt tcactgcgcg ctcatgaagc ccgcggaggt gcggctcgag
     3121 ccggaactgc gggcgttgac ggtgcagaag cctcgcatgc cgatcgtcgc caacatcgat
     3181 ggaacaccga agcgtgacgc ggctccggcc attgatgcct tggtgaaaca ggtgtcggcg
     3241 ccggtgcagt gggaggcggt cgtccactgc cttgcgtcgg aaggcgtcac cacgtatgtt
     3301 gaggtgggcc ctggcaacgt gctcggcggc atggtcaaga agatccacgc cgacgcgcgt
     3361 atcgtgcgtt ttggttctcc cgacgacctc gattcggtca tggcggcatg ttcgaactag
     3421 gtgaaaaagt gatcgtggtg acgggtgcgt ccaggggtat tggccgcggc atcgccgagg
     3481 cgctggcctc gcgcggcgcg cacgttgttg cggcggcacg cggggatcat gcctcgccgg
     3541 tcgtcgaagg catccgccag gccggcgggc atgcggaagg ggcgacggtg gacgtcaccg
     3601 acggcgcctc ggtcgaggct ctcgtggctg gggtcatcga ccgccacggc cgagtggatg
     3661 ggctggtcaa caacgccggc atcgcccgcg accagttgat gcttcggatg aagcgagagg
     3721 agtgggacga ggtgatcgcg accaacctca cggccgcgtt cacgtgcgtt catgcggtcc
     3781 tgaaaccgat gatcaagcgc cgggccggcc gcatcatcag tatcagctcg gtcgtcggac
     3841 agatgggcaa cgccggccag gcgaactacg cggcatccaa ggccgggttg atcggcttca
     3901 gcaaggcctt ggcgcgggaa gtcgcctcgc ggaacatcac ggtcaacgtg gtcgccccgg
     3961 ggctggttga caccgacatg accagggccg tgtcggacaa ggcccaggcg gaatggacgt
     4021 cgctgattcc gcttcaacgt ctgggcacgc cgcaggacat cgccgccgcc gtctgctttc
     4081 tcgcgtcgga tgaggcagcg tatattacag gtcaggtcct gaacgtcaac ggcggcatgt
     4141 atatgtagct cggttgatgg atggtggagg gtggatggtg ggaggatccc acgcgccgcc
     4201 aaccaccaac caccaactac caaccgaacg ggaggattga atggcggttt cagacaaggt
     4261 gaagggcatc atcgtggagc agcttggcgt ggacgaagag gaagtgacgc cggacgcgtc
     4321 gttcgtcgac gaccttgggg ccgactcgct cgataccgtg gagctcgtga tggcgttcga
     4381 ggaagagttc gggatcgaga tcccggacga ggacgccgag aagatcaccc gggtcaagga
     4441 agccatcgac tacatcgagg ctcacggcaa gaaaaagtag gcggaggggc agtgagcagg
     4501 cgggtcgtca tcaccggcat cgggttggtg tcgtcgctcg gcatcggcac cgacgccaac
     4561 tggaaggcgc tcgtggcggg gacgagcggg gtcgatcgga tctcgaagtt cgatgtcacg
     4621 gggttcgcag cgcagatcgc ggccgaagtc aggggattcg accctctcga ctacatcgac
     4681 aagaaagacg tcaagaagat ggacgtcttc atccagtacg ccgtcgccgc gtcgcagttc
     4741 gcgatggacg actcgcggct cgagatcacg ccatcgaacg cccccgacat cggcgtcttc
     4801 atcggctccg ggatcggagg cttccagacc atcgagcgtg agcacagcgc cctgctgaat
     4861 ggcggaccgc ggaagatctc gccgttcttc attccctcgg ccatcatcaa cctggcggcg
     4921 ggccaggtct cgatccggtt cggcgccaag gggccgaacc tggcgacgtg cacggcctgc
     4981 tcggcctccg cgcacgccat tggagaatcg tttgaaatca tcagacgcgg cgacgccgaa
     5041 gcgatgatcg ccggcggatc cgaggccgcg atcacgccga tgagcgtcgg cgggttcggc
     5101 gcgctgcgcg cgctgtcgac gcgcaacgac aacccgcgcg gcgcgagccg gcccttcgac
     5161 aaggatcgcg acgggttcat catcggcgag ggcgccggga tcctcatcct ggaggagctc
     5221 gactttgcgc gcaaacgagg ggcccgcgtg tacgccgagg tcgtcggcta cgggatgtcg
     5281 ggcgacgcgt atcacatcac ggcgccctct gaggacggcg acggcggcgt gcgtgtcatg
     5341 aggaaggcgg tctgcaaggc cgggattcca ccggacgagg tggactacat caacgcgcac
     5401 gggacgtcca cgccgtacaa cgatcgtctc gagacgctcg ccatcaagaa gctgttcggg
     5461 gaccacgcct tcaagatggc cgtgtcgtcg accaagtcga tgacggggca cctgctcggc
     5521 ggcgccggcg ggctggaggc cggcatctcg gcgcttgccg tgcaccatca ggtcgcgccg
     5581 cccaccatca acctcgacga tccggatccg gaatgcgacc tggactacgt cccccacaag
     5641 ggccggccga tgtcgattcg gtacgcgctg tcgaattcct tcggtttcgg cggcaccaac
     5701 gccgcgctgc tgttcaagaa attcgaaggg tagcgactcc gtggctgcgt ggagctgaga
     5761 gacccacgat atgaagatcg ccgtgtgcat caagcaggtc cccacgaggg aatggcagcc
     5821 gcggctgaga gaggacaagg cctggatccg cgagcaggac gccagcttcg agctgaacga
     5881 gcccgatgcg ttcgcgctcg aagaggcgct caggctgcgc gagaagcagg gcggggaggt
     5941 cgtcgcctgc gctgccggcc ccgcgcgggt gcagcaggtg atacgggagg cgctggcgcg
     6001 cggggctgac cgcgccctgc acgttgatga cgaggcgctc gcgagcgcgg acgcctcgat
     6061 cacgtcggcc gccctggcgt cggccatggc cgac
//
LOCUS       DBNGSH010000533         3217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2258539, whole genome shotgun sequence.
ACCESSION   DBNGSH010000533 DBNGSH010000000
VERSION     DBNGSH010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2258539"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="AC16CY_12285"
     CDS             complement(<1..1054)
                     /locus_tag="AC16CY_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR3354616.1"
                     /translation="MAFEAARPNEGSAFSRPIAGPVRLTPGLFDASPSTPASLTYEHY
                     YGLKEKAFSLSSDPRFLYKSPSHAPAFEELLNGIRRREGLIVLTGEIGTGKTTLIRSV
                     LNHLDRRTFTAFVPDPFVSREDLLKMLLIDFGVVSVTDLKSGSLSGASRPDLSYPLYE
                     FLDSLVPLQAFAVLIIDEAQNLPLPLLEEIRILSDLEHQEKLLQVVLVGQPELKSHLK
                     LPEMRQVDQRVSVRCELSALSHEDVAVYIRHRLAVASGGESRVDFSDDAIAAVFRGSG
                     GVPRVINLICDRALQRGQAAGSVRVERPLVLEAIRSLGLVPPIQVATTTGEAVPPPID
                     VEMRAHSVPVPAVDAPA"
     gene            complement(1476..2606)
                     /locus_tag="AC16CY_12290"
     CDS             complement(1476..2606)
                     /locus_tag="AC16CY_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3354617.1"
                     /translation="MSTTHRAECSAATLYVALELASREWLLTMSVGPEAKRQRARVRA
                     GECAGIQRALVQAKERFGLAADAAVCSCYEAGRDGFWPARLLTRLGVANLVVDSSSIE
                     VPRRARRAKTDRLDGEKLLRMLLRYWGGERQVWAVVRVPTPAAEDARHASRSLTTLQG
                     ERTRYRNRIHSLLALHGVARVRLDGRFPERLAAARDWAGEGLGPELQARVLEQWRVLQ
                     AVDAERRTRRRVERARVRATRTAGRTIAQRLAQVRGVAARAATILSDELFTRDLTSRR
                     EVGALSGLVSAPYQSGARRLDQGLSRSGIPAVRRIAVQLAWAWLRYQRTSAWSQWYQQ
                     RFGGGGPGLRKVGIVALARRLLIALWRYVTTGVVPDGALLKA"
     gene            complement(2745..>3217)
                     /locus_tag="AC16CY_12295"
     CDS             complement(2745..>3217)
                     /locus_tag="AC16CY_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease family protein"
                     /protein_id="HIR3354618.1"
                     /translation="ASVAAVGSAILTTALCAQLTKLLPLEDMGVSLRLGPDYFGYVLL
                     AAVPLCLFTAALQATVATMSRSFKEAQSYMGVLMLGPMLPGLVASLYPMGSTLWLNGV
                     PMLGSFLLLSDTLSGRPPGAAEFLTSAAVSFALTVGLLRVTTRLFRSEHIIFGR"
BASE COUNT          565 a         1154 c         1010 g          488 t
ORIGIN      
        1 ccgcgggcgc gtcgacggcc ggaaccggca cgctgtgcgc gcgcatctcg acgtcaatcg
       61 gcggtgggac ggcttctccc gtcgtcgttg cgacctggat gggaggcacg agccccaggc
      121 ttcggatcgc ctccagtacc agcggccgct ccacgcgcac cgaccccgcg gcctgaccgc
      181 gctggagcgc gcggtcgcag atcagattga tcactcgagg cacgccgccc gatccgcgga
      241 agaccgccgc gatcgcatcg tccgagaagt ccacgcggct ctcgcctccc gaagcgacgg
      301 caagccggtg acggatgtag acggcgacgt cctcgtgact cagcgccgac agctcgcatc
      361 gcaccgagac gcgctgatcg acctggcgca tttcgggcag cttcaggtgc gacttcagct
      421 cgggctgtcc gacgaggacg acctgcagaa gcttttcctg atgctcgagg tcggacagga
      481 tccggatttc ctcgagcagc ggcagtggca ggttctgcgc ctcgtcgatg atgaggacgg
      541 cgaacgcctg cagcggcacg agcgaatcga gaaactcgta cagcggatag ctcaggtcag
      601 gccgggacgc gccagagagg ctcccgctct tcaggtccgt gacggagacc acgccgaagt
      661 cgatcagcag catcttcagc aggtcttcgc gcgagacgaa cgggtcgggc acgaacgcgg
      721 tgaacgtgcg tcgatcgaga tgattcagca ccgagcggat cagcgtcgtc tttcccgtgc
      781 cgatctcgcc cgtcaacacg atcaacccct cgcggcgccg gatgccgttc aggagctctt
      841 cgaaggcggg ggcgtgcgac gggctcttgt agaggaaccg cggatcggac gagaggctga
      901 acgccttttc tttcagcccg tagtagtgct cgtaggtcag agatgcgggc gtcgagggcg
      961 atgcgtcgaa gagcccgggc gtcaggcgga ccggccccgc gatcggtcgg gaaaacgcgc
     1021 tgccttcgtt cgggcgcgca gcctcgaatg ccatgatgca caacctatcg gcagcaatgg
     1081 cttgcgactg cacacaacgt gcacattggc tacgttgtct ggcctggtcc ggctaaagcc
     1141 ggacactacg tacgcctcac gtccggcttc tatgagccgg actgatgtca agtctcctac
     1201 gtttcgacag acgtcgccgg agcaggcctc ctatcagcga tgaggtctgc ggaggtgacg
     1261 tctgtgttcg tcccccgcgc gcgatttcgt tcatcggcga gtggcctcgc acgagcttct
     1321 atgcgtgtcg gtcgagccga gcaccagatc ctctatgcgc ccgaggcgca gggacgaaat
     1381 cgtttcacac gcgccccttc taaagcccgg gtcacgcggt gaccctatcc ggaccacggg
     1441 cgacgtggtc gcgcgacgac tcgaggcccg cgcggctacg ctttcagcag ggccccgtcc
     1501 ggcaccacgc cggtggtcac ataccgccac agggcaatca gcaagcgtcg cgcgagcgcc
     1561 acgatgccga ctttgcgcag ccccgggccg ccccccccaa accgctgctg ataccactga
     1621 ctccaggcac tcgtgcgctg atagcgcagc cacgcccacg ccagctggac ggcgatgcgg
     1681 cgcaccgccg gaatcccact ccgactgagg ccctgatcga gccgccgcgc cccactctga
     1741 tacggcgcgg acacgaggcc gctcagcgcc cccacctcgc ggcgcgacgt cagatcacgc
     1801 gtgaacaatt cgtcgctgag gatcgtggcc gcgcgcgccg ccacgccccg tacctgcgcc
     1861 aagcgctgcg cgatcgtgcg ccccgcggta cgcgtcgcgc gcacccgcgc ccgctcgacg
     1921 cgccgccgcg tgcggcgctc cgcgtcgacc gcctgcagta cgcgccactg ctcaagcacc
     1981 cgcgcctgca gctccggccc gaggccctcg cccgcccaat cgcgcgcggc cgcaaggcgt
     2041 tcgggaaacc gcccgtcgag tcgcacccgc gcgaccccgt ggagcgcgag caaactgtga
     2101 atgcgatttc gatagcgcgt ccgctccccc tgcaacgtcg tcaggctgcg gctcgcatgc
     2161 cgcgcgtcct cggccgccgg cgtcggcact cgcaccaccg cccacacctg gcgctcgccg
     2221 ccccagtagc gcagcagcat ccgcagcagc ttctcgccgt cgagccgatc ggtcttggcg
     2281 cgccgggccc gccgcggcac ttcaatgctc gacgaatcga ccaccagatt cgcgacgccc
     2341 aggcgcgtca acaagcgcgc cggccagaac ccgtcgcgcc cggcttcgta acaactgcac
     2401 accgccgcgt ccgcggccag gccgaatcgc tccttcgcct gcaccaacgc ccgttggatc
     2461 cccgcgcact cgccggctcg gacacgtgcg cgctgccgct tcgcctcggg gcccacgctc
     2521 atcgtcaaca accactcccg gctggcgagc tccagtgcca catacaacgt cgctgcgcta
     2581 cactccgctc gatgggtcgt gctcatgtcg aactcctttc gaattagctc ttcaaaagag
     2641 tttcaacata cagccgaccc atcgtctgcc ctacataggg tctaaagccg gacactacgt
     2701 acgtttcagg tccagcgaaa gccgtagcct tccgcgctca ggaatcagcg cccaaagatg
     2761 atgtgttcgc ttcggaagag cctcgtcgtc actcgaagaa ggcccacggt cagggcgaac
     2821 gagacggccg ccgacgtcag gaattcggct gcgccaggcg gacggccgct cagggtatcc
     2881 gagaggagca ggaaggatcc gagcatcggt acgccgttca gccagagcgt gctgcccatc
     2941 ggatagagcg aggcgaccag gcccggcagc attgggccga gcatgaggac gcccatgtag
     3001 ctttgcgcct ccttgaaaga gcgcgacatt gtcgccaccg tcgcctggag ggcggccgtg
     3061 aacaggcaca gcgggacggc cgccaacagg acgtacccga agtaatcggg gccgaggcgc
     3121 aggctcacgc ccatatcctc gagcggcagg agctttgtca actgcgcgca caatgccgtc
     3181 gtcaggatgg cgctgccgac cgcggccacc gaagccg
//
LOCUS       DBNGSH010000534         2211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_220751, whole genome shotgun sequence.
ACCESSION   DBNGSH010000534 DBNGSH010000000
VERSION     DBNGSH010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_220751"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="AC16CY_12300"
     CDS             <1..812
                     /locus_tag="AC16CY_12300"
                     /inference="COORDINATES: protein motif:HMM:NF023431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HIR3354619.1"
                     /translation="MVKAMTEPQRKAAHELLKTGLSDRGYTTYTQIMQLESILRETEK
                     GSGPVRDPENYRFSIFGTPAAKGTWGWRVEFHHISLHFSVVNGTAISSTPSFAGANPA
                     EVREGPQKGHRTLGLLEDTARTLVTALDAGQRKTAIFNEVALNDIVTGNTLDINPLSP
                     AGLPAFAMTPAQREMLSKVLDAYAGLMAPEIAADRMAKIKIAGMDNITFAWAGSIERG
                     QRHYYRVQGPTFLIEFDNSQNNGNHVHSIWRDFRGDFGRDLLREHLRASAH"
     gene            850..1974
                     /locus_tag="AC16CY_12305"
     CDS             850..1974
                     /locus_tag="AC16CY_12305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3354620.1"
                     /translation="MRILSTILVTVALAVSAAADDGERILTIDHFVRVTSAVPALAGQ
                     PAQLYVRERVQAGMLARNARAADRVVLFVHGRGTPAEVAFDVPYEDYSWMAYLARAGF
                     DVFSVDMTGYGRSTRPAAMNDPCNLSANQQAAFVGRLIAAPCTASYPHAMTTIASDWA
                     DIGAAVDYIRALRGVERVSLVAWSLGGPRAGGYAARNPQKVHRLVFLAPAYGRASAAE
                     APAKPAVGTVFDTQSRADFDANWDRQIGCPAQFDRAVSDVVWSSMLESDPVGATWGSG
                     VRRAPNTTTWGWNAAAVARSQAPTLIVAGIHDKQVPPERPKELYEDLGAKQKVLIDLG
                     CASHNAMWEKNHTVLFRASLEWLEKGTVNGSESGVLRLGY"
     gene            1979..>2211
                     /locus_tag="AC16CY_12310"
     CDS             1979..>2211
                     /locus_tag="AC16CY_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3354621.1"
                     /translation="MPLRGRDHRTADHPIDALFLDRWSPRAMSGEPLSHDELMSLFEA
                     ARWAPSAGNTQPWRILYAHRDSEQWPSFFGLLV"
BASE COUNT          362 a          744 c          753 g          352 t
ORIGIN      
        1 tgatggtcaa ggcgatgacc gagccgcagc gcaaggccgc gcacgagctg ctgaagaccg
       61 gcttgagcga tcgcggctac acgacttaca cgcagatcat gcagctcgag agcatcctgc
      121 gagagaccga gaagggcagc ggcccggtcc gggatcccga gaactaccgc ttctcgatct
      181 tcggcacgcc ggcggccaag ggcacctggg gctggcgcgt cgagttccac cacatctcgc
      241 tgcatttctc ggtcgtgaac gggacggcga tttcgagcac gccctcgttt gccggcgcca
      301 accccgccga agtcagggag ggtccgcaga aggggcatcg gacgctcgga ctgctcgagg
      361 acacggcgcg cacgctcgtc accgcgctcg atgcggggca gcggaagacc gcgatcttca
      421 acgaggtcgc gctgaacgac atcgtgacgg gcaatacgct cgacatcaac ccgctgtctc
      481 cggccgggct gccggccttc gcgatgacgc cggcgcagcg cgagatgctg tcgaaggtgc
      541 tcgacgccta cgccggcttg atggcgcccg agatcgccgc cgaccggatg gcgaaaatca
      601 agatcgccgg catggacaac atcacgttcg cgtgggcggg gtcgatcgag cggggacagc
      661 ggcactacta ccgcgtgcag gggccgacgt tcctgatcga gttcgacaac agccagaaca
      721 acggcaatca cgtccactcg atctggcgcg acttccgagg cgacttcggc cgcgacctgc
      781 tgagggagca tctgcgggcg tcggcgcact gatcacggtc cggctgaagc cggacactac
      841 gtctcccgta tgagaattct ttccaccatc ctcgtgacgg ttgcgcttgc ggtgtcggcg
      901 gctgccgatg acggcgaacg catcctgacc atcgatcact tcgtacgcgt gacgtcggcc
      961 gtaccggctt tggcaggcca gccggcgcag ctctacgtgc gtgagcgtgt ccaggcgggc
     1021 atgctggcgc ggaatgcgcg ggcggccgac cgcgtcgtgc tcttcgtgca tggcagaggc
     1081 acgccagccg aggtcgcctt cgacgttccg tacgaagact acagctggat ggcgtacctg
     1141 gcgcgggccg gattcgacgt cttctcggtg gacatgaccg gctacgggcg atcgacgcgc
     1201 cccgcggcga tgaacgatcc gtgcaacctg tcggcgaacc agcaggcggc gttcgtcggc
     1261 cgcctcatcg cggcgccctg tacggcgtcc tatccccatg cgatgacgac catcgcgtcg
     1321 gactgggcgg atatcggggc cgccgtcgat tacatccgcg ccctgcgggg cgtcgagcgc
     1381 gtgagcctcg tggcctggtc gctcggcggc ccgcgcgccg gtggatacgc cgcccggaat
     1441 ccacagaagg ttcaccgcct cgtcttcctg gcgccggcct acggccgcgc gagcgccgcc
     1501 gaggcgccag ccaaacccgc cgtcggcacg gtgttcgaca cgcagtcgcg cgccgacttc
     1561 gacgcgaact gggatcgcca gatcggatgt ccagcccagt tcgaccgggc cgtgagcgac
     1621 gtcgtctggt cgtcgatgct cgagtcggat cctgtcggcg ccacgtgggg atcgggcgtt
     1681 cgccgcgcgc cgaacacgac gacgtgggga tggaacgccg cggccgtcgc caggagtcag
     1741 gcgccgacgc tcatcgtggc cggcatccac gacaaacagg tcccgcccga gcggccgaaa
     1801 gagctgtacg aggatctggg cgcgaagcag aaagtcctga tcgatctcgg ttgcgcctcg
     1861 cacaacgcga tgtgggagaa gaatcacacg gtgctcttcc gcgcctccct cgagtggctc
     1921 gagaagggga cggtgaacgg gtcagagtcg ggcgtcctcc ggttgggtta ctgatcccgt
     1981 gccgcttcgc ggacgcgatc accgcaccgc cgatcatccg atcgacgcgc tttttctgga
     2041 ccgctggtcg ccgcgcgcca tgtcgggcga gccgctctct cacgacgagc tgatgtcgct
     2101 cttcgaggcg gcgcggtggg cgccgtcggc cggcaacacg cagccgtggc gcatcctgta
     2161 cgcccaccgc gactccgagc agtggccgtc gttcttcggc ctgctcgtcg a
//
LOCUS       DBNGSH010000535         2195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_561000, whole genome shotgun sequence.
ACCESSION   DBNGSH010000535 DBNGSH010000000
VERSION     DBNGSH010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_561000"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="AC16CY_12315"
     CDS             <1..629
                     /locus_tag="AC16CY_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR3354622.1"
                     /translation="QAMAAVLGGTQSLHTNALDEALALPTEAAATLALRTQQIIAHET
                     GVADTVDPFGGSYFVEKLTSDLEDEAMAYFEKIDGMGGMVAAIERGFPQREIAESAYR
                     FQRAVEGKQQIVVGVNEFVSQEEASIGTLYVDDSVGERQLARLDLMRRRRDKARVERA
                     LDGLKTNAEHGANLMPALIEASRASATVGEMCDGLREVWGEYVEDPII"
     gene            637..1053
                     /locus_tag="AC16CY_12320"
     CDS             637..1053
                     /locus_tag="AC16CY_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR3354623.1"
                     /translation="MPAKIRVVIAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQTP
                     EQIVSAALQEDADVIGLSILSGAHMHICPRVMELLREQGLTDVLVVLGGIIPDADIPR
                     LNEIGIRGIFPPGTAMQEIVDFINRHARTRVGIGEA"
     gene            1057..2079
                     /locus_tag="AC16CY_12325"
     CDS             1057..2079
                     /locus_tag="AC16CY_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3354624.1"
                     /translation="MHKLLLADDSVTIQRVVELTFSGEDVQVVTVGDGEQAIARIPAE
                     LPDIVLADIGMPKRSGYDVSAFVKGRSDLAHIPVLLLAGAFEPVDEQRAQQVQSDGVL
                     VKPFEPQQVIARVRELIRGHKSGVPSPAAVAHAARPDAVRPHPDGALRSEPADWSHAP
                     AVETLPLRAAGPTTGGGETSGGDYFDRLDAAFAQLEIDPVRPAPAHPATEDEDRAHGV
                     PTVSKLLSETSLPTSLAPEPPPAEAPRPEPQPASDRNLVADMFAALFAMEQGESVTPV
                     PRQAAPGEAPMVTDALVDEVTRRVLARLAPDAANALVTHIVSEVAERLIRDEIARIKS
                     SVGSKL"
BASE COUNT          386 a          721 c          764 g          324 t
ORIGIN      
        1 tgcaggcgat ggcggcggtg ctgggcggca cgcagtcgct gcacaccaac gcgctggacg
       61 aagcgctcgc gctgccgaca gaagcggccg ccacgctggc gctgcggacc cagcagatca
      121 tcgcgcatga gacgggcgtc gccgacaccg tcgatccgtt cggcggatcg tatttcgtgg
      181 agaagctgac cagcgacctc gaggacgagg cgatggccta cttcgagaag atcgacggca
      241 tgggaggcat ggtcgccgcc atcgaacggg ggttcccgca gagggagatt gccgagagcg
      301 cgtaccggtt ccagcgggcg gtcgaaggca agcagcagat tgtcgtcggc gtcaacgagt
      361 tcgtgagcca ggaggaggcg tcgatcggca cgctctacgt ggacgactcg gttggcgagc
      421 gtcagctggc ccgcctggac ctgatgcggc gcaggcgcga caaggcgaga gtggaacggg
      481 cgctcgacgg gctgaagacg aacgcggagc acggcgccaa tctgatgccc gcgctgatcg
      541 aggcgtcacg ggcgtcggcg acggttggcg agatgtgcga cggtctccgg gaagtgtggg
      601 gcgagtacgt cgaggatccg ataatctgaa cctgtcatgc ccgccaagat ccgcgttgtg
      661 atcgcgaaac cgggactcga tggccacgac cgtggcgcca aggtgatagc ccgggcgctg
      721 cgcgacgccg gcatggaggt catttatacg ggtctgaggc agacgccgga gcagatcgtg
      781 tcagcggccc tgcaggagga cgccgatgtg atcggcctgt ccatcctctc cggcgcccat
      841 atgcacatct gcccacgggt catggagttg ctccgggagc agggtttgac cgacgtcctc
      901 gtcgtcctcg gcggcatcat tccggacgcc gacatcccaa ggttgaatga aatcgggatt
      961 cggggcatct ttccccccgg cacggcgatg caggagatcg tagacttcat caacaggcat
     1021 gcccggaccc gtgtgggcat cggagaggca tagggaatgc acaagctcct gctcgccgac
     1081 gacagcgtca cgattcaacg cgtcgtcgag ctgacgttct cgggcgagga cgtccaggtc
     1141 gtgaccgtcg gggacggcga acaggcgatc gcgcgcattc cggccgagtt gcccgacatc
     1201 gtgcttgccg acatcggcat gccgaagcgc agcggctacg acgtctccgc cttcgtgaag
     1261 ggccgatccg atctggccca catccccgtg ctccttctcg cgggggcgtt cgagccggtg
     1321 gacgagcagc gcgcgcagca ggtgcagagc gacggcgtgc tcgtgaagcc tttcgagccg
     1381 cagcaggtca tcgctcgtgt ccgggagctc atcagaggtc acaagtccgg cgtgccgtcg
     1441 ccagcggccg tcgcccacgc ggcgcgcccg gatgccgtcc ggccgcaccc ggatggcgcc
     1501 ctcagatccg agccggcaga ctggagccac gcgccggcgg tcgagaccct gccgttgaga
     1561 gccgcaggcc ccacgaccgg aggcggagaa acgagtggcg gtgactactt cgatcggctc
     1621 gacgccgcgt ttgcgcagct cgagatcgac cccgtccgtc cggcgccggc tcacccggcg
     1681 accgaggacg aggatcgcgc acacggggtc ccgaccgtct cgaagctgct gtcggagacc
     1741 tcgctgccga cctcgcttgc acccgaaccg ccgccggcag aggcgccgcg cccggaaccg
     1801 caacccgcct cggaccgcaa cctcgtagcc gacatgtttg ccgcgctgtt cgcaatggag
     1861 cagggcgaat cggtcacgcc cgtgccgcgc caggcggcgc ccggcgaggc gcctatggtg
     1921 accgacgcgc tcgtggacga ggtgacccgc cgcgtcctgg ctcggctcgc cccggacgcg
     1981 gccaatgcgc tcgtcacgca cattgtctcg gaagtggccg agcgcctgat tcgcgacgag
     2041 atcgcccgaa tcaagtcctc cgtgggcagc aagctctgag cacattggat caacacccga
     2101 tcaccctccc cgagaaaccg gcgctcgaag gcctcgaggc gaagtggacc gcccgatggg
     2161 aagccgatgg cacgtaccgg ttcgatcgga atcac
//
LOCUS       DBNGSH010000536         1782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2258952, whole genome shotgun sequence.
ACCESSION   DBNGSH010000536 DBNGSH010000000
VERSION     DBNGSH010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2258952"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="AC16CY_12330"
     CDS             complement(<1..1254)
                     /locus_tag="AC16CY_12330"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3354625.1"
                     /translation="MFVTKKHLPRRTFLRGMGVSLALPLLDSMIPARTLLAQTAARPV
                     PRLGFIYVPHGAIMDKWTPAAIGDGFEFTPILKPLEPFRDRLNVVSGLGHRAADTTAV
                     HSLSPTTWLSGVRPKATQGVDAYAGVTADQVAAQAIGQDTILPSMELASEDHSGLIGS
                     CDRDYGCIYLNTLSWRTPTTPMPMEINPRKVFERMFGQGGSASERLARIQEDRSILDA
                     ITKDAASLELKLGASDRQVMSQYLENVREIERRIERAESSQGDQDLLLPARPAGVPFD
                     YEEHIKLMYELMALAYQADVTRVITFMISREVSNRTYPQVGVTDGHHAISHHQNRAEK
                     MEKNVRIQTYNIGQFSYFLEKLRDTPDGDGSLLDHALLLYGSNMSNSNAHDHYPLPNV
                     VIGGAAGRMKGGRHLKYADHTPMTNL"
     gene            complement(1324..>1782)
                     /locus_tag="AC16CY_12335"
     CDS             complement(1324..>1782)
                     /locus_tag="AC16CY_12335"
                     /inference="COORDINATES: protein motif:HMM:NF019253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HIR3354626.1"
                     /translation="DVPDLTENEPGQEAQSLRVRLEAHRRNPTCATCHRVMDPLGFAL
                     ENFDGVGEWRVTEPGGRVDPVGQLADGAAVDGPIGLRRAVLQHPEMFVRTVTQKLMTY
                     GLGRGVEYEDMPLVRRIARDAAQREYKWSAIVLGLVRSAPFQMKKAQQGT"
BASE COUNT          264 a          611 c          586 g          321 t
ORIGIN      
        1 caggttcgtc atcggcgtgt gatcggcgta cttcagatgc cggccgccct tcatccggcc
       61 cgccgcgccg ccaatcacca cgttgggcag cgggtagtga tcgtgggcgt tgctgttgct
      121 catgttgctg ccgtagagca gcagggcgtg atccagcagc gatccgtcac cgtccggcgt
      181 gtcgcgcagc ttctcgagga agtagctgaa ctgcccgatg ttgtaggtct ggatccggac
      241 gttcttctcc atcttctccg cgcggttctg atggtgcgag atcgcatgat ggccgtcggt
      301 cacgccgacc tgcggatacg tccggttgct cacctcgcga gaaatcatga aggtgatgac
      361 gcgcgtgacg tcggcctgat acgccagcgc catcagctcg tacatcagct tgatgtgctc
      421 ctcgtagtcg aacggcacgc ccgcggggcg ggccggcagc agcaggtcct gatcgccctg
      481 gctcgactcg gcgcgttcga tgcgccgctc gatctcgcgg acgttctcga ggtactggga
      541 catcacctgc cgatcggagg cgccgagctt gagctccagg ctggcggcgt ccttcgtgat
      601 ggcgtcgagg atgctgcgat cctcctggat ccgcgcgagc cgttcggagg cgctgccgcc
      661 ctggccgaac atccgctcga acaccttgcg cggattgatc tccatcggca tgggcgtcgt
      721 cggcgtccgc cacgacagcg tgttcaggta aatgcagccg tagtcgcggt cgcacgagcc
      781 gatgaggccc gaatggtcct cgctggccag ctccatcgac gggaggatcg tgtcctgccc
      841 gatcgcctgc gcggcaacct ggtcggcggt gacgccggcg tacgcgtcca cgccctgtgt
      901 cgccttgggc cgcactccgc tcaaccacgt cgtcgggctg agcgagtgga cggcggtcgt
      961 atcagcggcg cggtggccga gcccgctgac gacgttcagc cggtcgcgga acggctcgag
     1021 cggcttgagg atcggggtga actcgaagcc gtcgccgatg gcggccggcg tccacttgtc
     1081 catgatggcg ccgtggggca cgtagatgaa gcccaggcgc ggcaccgggc gcgcggcggt
     1141 ctgcgccagc agcgtgcgcg ccgggatcat cgagtcgagc agcggcagcg ccaggctcac
     1201 gcccatccct cgcaggaacg tccttcgcgg cagatgcttc ttcgtgacga acatgtcagt
     1261 taccctcacg gtccggctga agccggacac tacgtacgct cacggtccgg ctgaagccgg
     1321 acactacgta ccctgctgcg ccttcttcat ctggaagggc gcgcttcgca cgaggcccag
     1381 cacaatcgcc gaccacttgt actcgcgctg tgccgcgtcg cgggcaatcc ggcgaacgag
     1441 cggcatgtcc tcgtactcta ctccgcggcc aagaccgtag gtcatcagct tctgcgtcac
     1501 cgtgcgcacg aacatctccg ggtgctgcag caccgccctg cgcaggccga tcggcccgtc
     1561 aacggcggcg ccgtcggcca gttggcccac cgggtcgacg cggccacctg gctccgtgac
     1621 acgccactct ccgactccat cgaagttctc gagcgcaaac ccgagcggat ccatcacgcg
     1681 gtgacaggtc gcgcacgtcg ggttccgccg gtgcgcctcc agccggacgc gcagcgactg
     1741 tgcttcctgc cccggctcgt tctccgtcag gtccgggacg tc
//
LOCUS       DBNGSH010000537         2744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_221571, whole genome shotgun sequence.
ACCESSION   DBNGSH010000537 DBNGSH010000000
VERSION     DBNGSH010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_221571"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="AC16CY_12340"
     CDS             <1..725
                     /locus_tag="AC16CY_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354627.1"
                     /translation="QGVTAAYEGWFKNADGSFSLLVGYFNRNQKQTLDIPAGPNNRIE
                     PGGPDQGQPTHFMPRRQWGVFTVRVPADFGDRKLTWTIVANKHTTSVAMGLHPDYQVE
                     PLRDAAEGNTPPLVRLEPGGRAFQGPPLEVARALTTATARPLTLTLWATDDGKVNSGQ
                     RATDSPITVSWSKFRGPGDVSFANPRPPVDKADGRAETRATFGAAGEYILRGQVNDVS
                     GDGGGGFQCCWTNVHVKVTVKP"
     gene            complement(709..2484)
                     /locus_tag="AC16CY_12345"
     CDS             complement(709..2484)
                     /locus_tag="AC16CY_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3354628.1"
                     /translation="METELFRFATFELDCRSRELRDGARRVRLQDQPYEILRLMLDRP
                     GDVVTRDELRQRLWPDGTFVDFEHSLNAAIKRLRAALGDEADQPRFVETVPRRGYRFI
                     ARLERGQAVAGPPAPPLHRLLVLPFSNLSDDSSHEYFSDGLTEELIAQLGPLCRGKVA
                     VIARWSSMVYKGSLQRAREIGEALQADYLLEGSARKDGPRVRITARLVEAASEAYLWS
                     ETYERTANDWLGVQADVAGRVARSLIKELVPGPTPIRTAEEQPAAYQAYLRGRYHWAR
                     PGDVGLQDSLRSFEEATRLAPDFAAAHGGLARVRVGRAEYYREVPRLALTSAREAAMR
                     ALALDPSVSEAYCVIGDARRMLDMDWPGAEAAYVQALALNPSNEVATRQYGILLALRS
                     RAEEACWQIDRACELDPLCLVANTAAAWARYTDGRVGLAIDRCKYTLDLDPEFGPALR
                     ILAAARLAEGRTADARAALESAVAADASDPVRLSWLAYVRAIEGAHREARDLVARARA
                     LGAERYVSAYHLAVAFVGLEDHNEAFAQLDRAWLDRDPALAGIVIEPLFEPIRKDSRY
                     AALTARLNGGSAERGLQPARPTASP"
BASE COUNT          431 a          965 c          943 g          405 t
ORIGIN      
        1 gccagggcgt caccgcggcg tacgaaggct ggttcaaaaa cgctgacggc agcttttcgc
       61 tgctcgtcgg atacttcaac cggaaccaga agcagacgct cgacattcca gcgggaccga
      121 acaatcgcat cgagccgggg ggccccgacc agggccagcc cacgcatttc atgccccgcc
      181 gccaatgggg cgtcttcacc gtccgcgtgc cggccgattt cggcgatcgc aagctcacct
      241 ggacgatcgt ggccaacaag cacacgacgt cggtggcgat gggcctgcac cccgactacc
      301 aggtggaacc gcttcgcgac gcagccgaag ggaacacccc gcccctcgtc cggctcgagc
      361 ctggcggccg cgcgtttcaa ggcccgccgc tggaggtcgc gcgggcgctg acgacggcca
      421 cggcaaggcc gttgacgctc acgctgtggg cgaccgacga cggcaaggtc aactccggcc
      481 agcgggcaac cgacagtccg atcactgtga gctggagcaa gttccgcggc cccggcgacg
      541 tctcgttcgc caatccacgg ccgccggttg acaaagccga cggccgcgcg gagacgaggg
      601 cgacattcgg cgccgccggc gagtacatcc ttcgcgggca ggtcaacgac gtgtcaggcg
      661 acggcggcgg cggctttcag tgctgctgga cgaacgtcca cgtgaaggtt acggtgaagc
      721 cgtaggacgc gcgggctgaa gcccgcgctc tgcgctgccg ccgttcaacc gcgcggtcag
      781 cgcggcgtac ctcgagtcct tgcgaatcgg ttcgaacagc ggctcgatca cgatgcccgc
      841 cagcgccgga tcgcgatcga gccacgcgcg atcgagctgg gcgaacgcct cgttgtggtc
      901 ctcgaggccg acaaacgcca cggccaggtg gtaggcggac acgtaacgct cggctccgag
      961 cgctcgcgcg cgcgcgacga gatcgcgcgc ctcgcgatgc gccccttcaa tcgccctgac
     1021 gtacgccagc cacgacaagc gaaccggatc gctggcgtct gccgccacgg ctgattcgag
     1081 cgccgctcga gcgtcggccg tcctgccctc ggcgaggcgg gcggcggcaa ggatccgcag
     1141 cgccgggccg aactcggggt ccaggtcgag cgtgtacttg cagcggtcga tcgccaggcc
     1201 gacgcggccg tcggtgtacc gcgcccaggc ggccgccgtg ttcgcgacga ggcacagcgg
     1261 gtcgagctcg cacgcgcgat cgatctgcca gcacgcttcc tcggcccgcg agcgaagggc
     1321 cagcaggatg ccgtactgcc gcgtggcgac ttcgttgctc ggattgagcg cgagtgcctg
     1381 gacgtaggcg gcctcggcgc ccggccagtc catgtcgagc atgcgccgcg cgtcgccgat
     1441 gacgcagtac gcctcggaga ccgacggatc gagcgcgagc gcgcgcatcg ccgcctcgcg
     1501 agccgaggtc agggcaaggc gggggacctc gcggtagtac tccgccctgc cgaccctgac
     1561 gcgcgccagc ccgccgtggg cggcggcaaa gtcgggggcc agccgcgtcg cctcctcgaa
     1621 gctccggagc gagtcctgca gtcccacgtc gcccggcctc gcccagtgat agcgtccccg
     1681 caaataggcc tgatatgcgg ccggctgctc ctccgccgtc ctgatcggag tgggaccggg
     1741 caccagttcc ttgatcagcg agcgggcgac gcgcccggcg acgtcggcct ggacaccgag
     1801 ccagtcgttc gcggtgcgct cgtaggtctc agaccagagg tacgcctcgc ttgccgcttc
     1861 gacgagccgg gccgtgatgc gtacgcgcgg cccgtccttc cgggcgctgc cttcgaggag
     1921 atagtcggct tgcagagcct cgccgatctc tcgcgcgcgc tgcaggctgc ccttgtagac
     1981 catcgaggac caccgcgcga tcaccgccac cttgccgcgg cacagcggcc cgagctgggc
     2041 gatgagctcc tcggtgagcc cgtcgctgaa gtactcgtgg ctgctgtcgt cgctcaggtt
     2101 gctgaacggg agcacgagca ggcgatggag cggcggggcg ggcggaccgg ccacggcctg
     2161 tccacgctcg agcctggcga tgaagcgata cccccgccgg ggcacggtct cgacgaacct
     2221 gggctggtcg gcctcgtcgc cgagcgccgc tcgcagccgc ttgatggcgg cgttgaggct
     2281 gtgctcgaag tcaacgaagg tcccgtcggg ccacaaccgc tgcctgagct cgtcgcgtgt
     2341 gacaacgtcg ccggggcggt cgagcatcag gcggaggatc tcgtacggct gatcctgcag
     2401 gcggaccctg cgggccccgt cgcgcagctc gcgggaacgg cagtccagct cgaaggtcgc
     2461 gaatcgaaac agctcggtct ccatgcctca cgcctcgtac cgtcccacct attctcgacc
     2521 gaaacgggga cgtttccgaa tgtggcgatg acaagtcacc aacatgtcac ctcgggttac
     2581 ctcctggtgc attgaggcac ttccgaccgg attccagaat cgactcgtct ccgcgcggtg
     2641 cgcggatcga ccttccacag gagcccggtc atgtccgctc gcgttgtcct cgcggtggct
     2701 ttctcgacgg tagtggcgac gccgtgcgcg caagcggcgg aaac
//
LOCUS       DBNGSH010000538         2767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1750140, whole genome shotgun sequence.
ACCESSION   DBNGSH010000538 DBNGSH010000000
VERSION     DBNGSH010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1750140"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="AC16CY_12350"
     CDS             <1..1244
                     /locus_tag="AC16CY_12350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HIR3354629.1"
                     /translation="HYASREHALEERAVLSREAWTAFFGERDGFLVALTSIHWREAWK
                     YGERAFRYCQHDAGHAIGALRYAAALFGWTMRVLPEWSDEQIAALLGVDREGDRARDA
                     DREEPECLALVAPGPGRAGDPAALVAAARHAAWHGRANRLSAAHVDWPVIDRVAEATR
                     YPGSTPNTPTPNSQSRPVEELGVGNWELGVAERPNARDVILRRRSAVAFDGRSALSKE
                     RFVAMLSRLCPAGPPWDAIDWPPHVHLAVFVHRVEGLVPGVYAYLRDDSVLDVWRSAM
                     RPEFLWERVSNDRLFLLLPHDVTWAAARVSCDQDIAGAGFFSLGMIARLASSLQERGE
                     WFYRRLFWECGVIGQVLYLEAEAAGARATGIGCFYDDPVHELLGLSGPEWQSLYHFSM
                     GVPVEDDRLTTEPGYAWERSL"
     gene            1379..1774
                     /locus_tag="AC16CY_12355"
     CDS             1379..1774
                     /locus_tag="AC16CY_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3354630.1"
                     /translation="MDGWLGVVIMIALGVGFAGVMIGLSLLLGPKNPTPEKLAPYECG
                     MPAVGDARERHSVKFYLVAMIFLLFDIEIAFLYPWAVALPSLGWAGFVQIVTFFLVLA
                     VGYVYVWRKGVLDWGPARRARTRPEQKAA"
     gene            1777..2256
                     /locus_tag="AC16CY_12360"
     CDS             1777..2256
                     /locus_tag="AC16CY_12360"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3354631.1"
                     /translation="MAETHEHGLDPELPLLTTTVERLVQWARRSAIWPAQFGLACCAI
                     EMMAMAASRYDIARFGAEVFRGSPRQSDLMIVAGRLSRKMAPAMRRVYDQMPDPKWVI
                     SMGACASVGGVFDNYAIVQGVDQVVPVDVFVPGCPPRPESLIYGIVLLQQKIDRSRA"
     gene            2271..>2767
                     /locus_tag="AC16CY_12365"
     CDS             2271..>2767
                     /locus_tag="AC16CY_12365"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3354632.1"
                     /translation="MDSSSIINTLQPLVPGAALEAGRSVDFPTLYVPADRLVETCLAL
                     RDGPSLRFNVLTEITAADYLPRDPRFEVVYHLLSIPHRARLRLKVRVNAAGYVPTVQG
                     VWRGAAWPEREVWDMFGIVFDGHPDLRRLLMPEDWEGHPARKDYPVQIRKAAQTYEPL
                     EVSEE"
BASE COUNT          411 a          896 c          962 g          498 t
ORIGIN      
        1 gccactacgc atcccgcgag cacgcgctcg aagagcgcgc cgtcctgtcg cgggaggcct
       61 ggacggcctt cttcggcgag cgcgatggct tcctcgtggc gctcacctcc attcactggc
      121 gagaggcttg gaaatacggc gagcgcgcgt tcaggtactg ccagcacgac gcgggacacg
      181 cgatcggcgc gctgcgttac gccgccgcgc tcttcggctg gacgatgagg gtgctgccgg
      241 agtggtcgga cgagcagatc gccgccctgc tcggagtcga cagagaaggc gatcgagcga
      301 gagatgccga tcgggaagag ccggagtgtc tcgcgctcgt ggctcccggc ccaggccgcg
      361 caggcgatcc agcggcactt gtcgccgcgg cgcgtcacgc cgcatggcac ggcagggcga
      421 accggctgag cgccgcccac gtagactggc cggtcatcga tcgagtcgcg gaggcgacga
      481 ggtatcccgg ttcaaccccc aacactccaa ctcccaactc ccaaagccga ccggtcgaag
      541 agttgggagt tgggaattgg gaattgggag ttgcggagcg accgaacgcc cgcgacgtga
      601 tcctccgtcg ccgcagcgcc gtcgccttcg acgggcgatc ggcgctgtcc aaagagcggt
      661 tcgttgcgat gctgtcgagg ctctgcccgg ctggaccgcc ctgggacgcg atcgactggc
      721 cgccgcacgt ccacctcgcc gtgttcgtgc accgcgtcga ggggctcgtg cccggggtgt
      781 acgcttacct tcgcgatgac tccgtgctcg acgtctggcg atcggccatg cgccccgagt
      841 tcctctggga gcgcgtgtcg aacgatcggc tgttccttct gctcccccac gacgtgacgt
      901 gggctgccgc acgcgtttcc tgcgatcagg acatcgcggg cgccggattc ttcagcctcg
      961 gcatgattgc gaggctggca tcgtcgctcc aggagcgggg cgagtggttt tatcgccgtc
     1021 tgttctggga gtgcggcgtg atcggccagg tgctgtatct cgaagccgag gcggccggcg
     1081 cacgcgccac gggcatcggc tgcttctacg acgatccggt tcacgagctg ctgggtctgt
     1141 caggccccga gtggcagagt ctctatcact tctcgatggg ggtgccggtc gaggacgatc
     1201 ggctcacgac ggagcccggc tacgcgtggg agagaagcct ctagcaggct ctctcccaaa
     1261 ttgacgtttc cgggcggctt ctatagcatg aacaggtttg cgaaactctt cacaagcgcg
     1321 gctgtcgcgc gcggcggcgg gtgtgatttc tgtcaccgcc cgtcgtaccg agggataaat
     1381 ggacggctgg cttggcgtgg tgatcatgat cgccctcggc gtgggattcg ccggggtgat
     1441 gatcgggttg tcgctcctgc tcggccccaa gaatccaacc cctgagaagc tggcgcccta
     1501 cgagtgcggc atgcccgccg tcggggacgc gcgcgagcgc cactcggtca agttctacct
     1561 cgtggcgatg atcttcctgc tcttcgacat cgagattgcc ttcctctacc cgtgggcggt
     1621 cgcgctgccg agcctggggt gggccggctt cgtgcagatc gtcacgttct tcctggtgct
     1681 ggcggtcggc tacgtgtacg tgtggcgcaa gggcgtgctc gactggggcc ccgcgcgccg
     1741 cgcgcgcacc cggccagaac agaaggccgc ctgaacatgg ctgagaccca cgaacacggc
     1801 ctcgatccgg agcttccgct cctgaccacg accgttgagc ggctcgtcca gtgggcgagg
     1861 cgatcggcga tctggccggc gcaattcggt ctcgcctgct gcgcgatcga gatgatggcg
     1921 atggcggcca gccggtacga catcgcgcgg ttcggcgcgg aggtgttccg gggctccccg
     1981 cgccagtccg acctgatgat cgtcgcgggc cgcctgtcca ggaagatggc gcccgccatg
     2041 cggcgcgtct acgaccagat gccggacccg aagtgggtga tttcgatggg cgcctgcgcg
     2101 tcggtcggcg gcgtgttcga caactacgcg atcgtccagg gcgttgacca ggtcgtcccc
     2161 gtggacgtgt tcgtgcccgg gtgtcctccg aggcccgagt cgctcatcta cggcatcgtg
     2221 ctgttgcagc agaagatcga ccgatcgcgg gcataagact cttcgtcagc atggattcct
     2281 cgtccatcat caacacgttg cagccgctcg tgccgggggc ggcgttggaa gccggacgga
     2341 gcgtggactt cccgacgctg tacgtgccgg ccgatcggct cgtcgagacg tgcctcgcgc
     2401 tgcgcgacgg cccgtcgctg cgcttcaacg tgctgaccga gatcaccgcg gccgactacc
     2461 tgccgcgcga tcccaggttc gaggtcgtct atcacctgtt gtcgatccct cacagggcgc
     2521 gcctgcggct gaaagtgcgc gtgaacgcgg ccggctacgt tccgacggtc cagggtgtct
     2581 ggcggggcgc cgcctggccg gaacgggaag tgtgggacat gttcggcatc gtgttcgacg
     2641 gccatcccga cctgcggcgg ctgctgatgc ccgaggattg ggaagggcat ccggcgcgca
     2701 aggactatcc cgtgcagatt cgcaaggccg ctcagaccta cgagccgctc gaggtcagcg
     2761 aggaaga
//
LOCUS       DBNGSH010000539         6864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2259811, whole genome shotgun sequence.
ACCESSION   DBNGSH010000539 DBNGSH010000000
VERSION     DBNGSH010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2259811"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="AC16CY_12370"
     CDS             <1..999
                     /locus_tag="AC16CY_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354633.1"
                     /translation="SISVFRRPRPVANTKYTTHQNFGVGPFIAKSGLPTATDPITGRP
                     TSMNLMADARVPTLAVQAADAAAQHLEVTGPLTASQIARIVEFEGQVYVAQVSSTAAG
                     SLIEPDGPPAFGPRHLAAGDAGVLGNNTTRYVFPMGGKWAQLPRPGGEVENARNAARE
                     SIARGHDVFMFRTFWIRDSMHLNTVGLGNPAKRTCATCHGMHMTGMDTANGWMDIGTT
                     NLPWASEVPLNPWNAAKPQMPLFRITCKPEVKPHPFLGRVIYTQDPGRALISGRCNDV
                     GTIVMQQFRGLAARAPYFSNGSAATLRELVDFYDRRYNIGYSEQDKQDLVNFLGAL"
     gene            999..1799
                     /locus_tag="AC16CY_12375"
     CDS             999..1799
                     /locus_tag="AC16CY_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354634.1"
                     /translation="MTVGRARRGSVLLVGWLAAGAVGVAQQRTNFVSCPIVRDTASVP
                     CWLAEYEGELYFLTLQTDVSAPVTPPWLGHRVLVEGTVSAEPRICGGIVLQPVHLSVL
                     DRDASCNTMLPAEERYNLTFEPPRPPGPSRGRLAFDNAPAASPSPATDSTREFVVSYP
                     FDGLVGFNHAGLLTQVLEFARAANASEIEIVGYRGAVRLSNGQTMVEESAIGKKRAEQ
                     VAMLLQGASLTAPSYKVAWRDEAASASGVDDHLSRRVVITVRPLQSRR"
     gene            complement(1806..3431)
                     /locus_tag="AC16CY_12380"
     CDS             complement(1806..3431)
                     /locus_tag="AC16CY_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354635.1"
                     /translation="MYNRVVVLSLVAAISTALAIRLAGQSAAPERQLAARAAAALGGR
                     DRVMALKTLQVVGYGELAYFNGGGNITGDPAAPQKWQTVLEYTRTIDLEHWRTRVQQR
                     LKMDFVFASTIGQLGLNRTNETLDGDVAYNFGGGFLAAPQSGTPQPQLAGAAAVRQRR
                     VELLAHPITIVRAALDATSKLSNLRKQGNLQLVDVTVRQGDTLTLAVDATTSLPAWVS
                     YVGPNANLGDLTYRTAFAGYVPEKGIQLPTGFATTIDFRNVVQSKLYVDRNIVDAPID
                     DLSAPASVRSAAPQGAPPGGNANLKPMKVADHVWYMNGNTFFEFDDHITMVEANRPDA
                     ALQAILQVVNALVPGKRVTQVIQSHHHFDHSVGLRAAVAQGLTLISRRGNEGIFREMV
                     ARPAKLFPDALGRSPKPLKFIPVDDHLELKDSTNEIDIYHIVGNYHMADGVIVHVPAS
                     NLLIEADLTTQDWDFNWWGDSLMNNIEYRKIKVDTNLAVHAQKPFPLAEVVSAIERQV
                     RNAQAFCRRAAEAQFFQPGCPIQYNRPLPPSPN"
     gene            3629..4462
                     /locus_tag="AC16CY_12385"
     CDS             3629..4462
                     /locus_tag="AC16CY_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354636.1"
                     /translation="MIVVVVGASGSSGAQWFKVPSPRAPRTATGDVDLTAAPPRLANG
                     RPDLSGVWMTAEPLCVIRGTAPISELLKMNPPSRKCPPRTASFSRQSINIGIDMPGGL
                     PYQPWLATLVDERTANQAVDDPHIRCLPDFFLRAYGLPHYLKFVQTPDLLVMLNEYNG
                     TYRQVFTDGRQLPEAPNPSWQGYSTATWADDTLVVDSIGFRDDLWIDWDGSMVTGTAK
                     VRERIRRPAFGHLEIEVTVDDPKAYTKPWSVTLRQQFAADTELIDEICAEGERFVETL
                     K"
     gene            complement(4471..5457)
                     /locus_tag="AC16CY_12390"
     CDS             complement(4471..5457)
                     /locus_tag="AC16CY_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354637.1"
                     /translation="MMMSTRCAVIVLMCAAAVRLAAQQSQPPAPAKPDSPAIIEQLDR
                     LKTSVGPRWADAVHFWCEAPRANRADDPVIQPAKVFDNLYAIGNVGTVAYVIQTSAGL
                     MMIDALGANQVESDLVPGFAKLGLDPAQVKVILVGHGHADHFGGSAYFQQKFGAKVYV
                     SAADWTLMETPPRGRGPGAPPPAAPPKRDGELRDGESVTLGNVSVRAVAVPGHTPGSM
                     GFVIPVVDRGERHVAALFGGSWLTPGLLNDEAMKTYIASVSHFRQATVDAGVDAWLQN
                     HPLMVPFQGWLTRLSSRGRNDANPFVVGRAEYQKFLDVMDGCSRIALARRAL"
     gene            complement(5471..>6864)
                     /locus_tag="AC16CY_12395"
     CDS             complement(5471..>6864)
                     /locus_tag="AC16CY_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354638.1"
                     /translation="YAPHSWMGFSTGRFVGNALEVQTTHLKQGWYRRNGTPESDQAML
                     VEFFVRHGDRLTHTVVVTDPVYLEEPLVTTTDFFRQPADHQNWLFPCDDGEQVLGRAP
                     DDVPSYLFGQNPFVKEYRTKYTLAAVGHLGGAASIQPEIVSMLAAAKDDSSLERPSRG
                     PQATSKALPVEPADGDIHILPVRGNVYMLVGDGGNIVAQVGNEGVFVVDTGTGARAAR
                     TLAAIRTLSDKPVQFIANTSFLPDRTGGNEALRKAGADPSVRGTFFSLQFRDAGVGAT
                     IMAHQNVQNRLNAAKRPADALPTDTFLEERRRTFHNDDAIEMLWQPNAITDGDSLVHF
                     RRADVIVTGNIFTTTQYPFIDVADGGTVEGEIRALNQILERTVFEHQGQGGTLVVPGH
                     GYLSDEHEVVEYRDMVVIVRDRIRALVRAGGTLEQVKAARPTADYDTRYGATTGPWTT
                     DMFVEAVYRTIRR"
BASE COUNT         1179 a         2219 c         2234 g         1232 t
ORIGIN      
        1 tcgatttcgg tgttccgccg cccgcgcccg gtcgcaaaca cgaaatacac cacccatcag
       61 aacttcggcg tcggtccgtt catcgccaag agcggcctgc cgacggccac cgatccgatc
      121 acgggcaggc cgacgagcat gaacctgatg gccgatgcgc gcgtgccgac gctggccgtg
      181 caggcggcag acgcggcggc acagcatctc gaggtcacgg gcccgctcac cgcctcgcag
      241 atcgcgcgca tcgtcgaatt cgaaggacag gtctacgtcg cgcaggtctc gagcacggcc
      301 gccggcagcc tgattgagcc cgatggccct ccagcgttcg ggccacgaca tcttgcggcc
      361 ggtgatgctg gcgtgctcgg gaacaacacc acgcgttatg tcttcccgat gggcggcaaa
      421 tgggcgcagc tgccgcgccc ggggggcgag gtggagaacg cgcgcaacgc cgcgcgcgag
      481 tcgatcgccc gcgggcacga cgtgttcatg ttccgcacgt tctggatcag ggactcgatg
      541 cacctcaaca cggtgggact gggcaacccg gccaaacgaa cctgcgccac ctgtcatggg
      601 atgcacatga ccggcatgga cacggccaac ggctggatgg acatcggcac gacgaatctc
      661 ccctgggcct cggaggtgcc gttgaacccc tggaacgcgg cgaaaccgca gatgccgctg
      721 ttccgcatca cgtgcaaacc ggaagtgaag ccgcatccgt ttctgggccg cgtcatctac
      781 acacaggacc ccgggcgggc gttgatctcc ggccggtgca acgacgtcgg cacgatcgtc
      841 atgcagcagt tccgggggct cgcggcgcgg gcgccctatt tctcgaatgg gtcggcggcg
      901 acgctgcggg aactggtgga cttctacgat cgccgctaca acatcggcta ctccgagcag
      961 gacaagcagg acctggtcaa ctttctgggg gcactctgat gactgtcggt cgcgcgcgtc
     1021 gcggtagtgt gctgctggta ggctggttgg cagcaggggc ggtcggcgtt gcccagcagc
     1081 ggacgaactt cgtgagctgt ccgatcgttc gggacacggc gtcggtcccg tgctggcttg
     1141 cggagtacga gggcgagctg tacttcctca ctctccagac cgacgtcagt gcgccggtca
     1201 caccgccatg gctcggccat cgcgtgctgg tcgaaggcac ggtgtctgcc gaacctcgga
     1261 tctgcggtgg catcgtgctg cagccggtgc acttgtccgt gctcgaccgc gacgcctcgt
     1321 gcaacacgat gctcccggcc gaagagcgct acaacctcac gttcgagccg ccgcgccctc
     1381 ccgggcccag caggggtcgc ctggccttcg acaacgcccc ggctgcctcc ccctctccgg
     1441 ccaccgactc aacaagggag ttcgtcgtgt cctacccctt cgacggcctg gtcggattca
     1501 accatgcggg cctgctcacc caggttctgg agttcgcccg ggcggcgaac gcgagcgaga
     1561 tcgagatcgt gggttatcgc ggcgccgtgc gtctctcgaa cggacagacg atggtcgagg
     1621 aatccgccat cgggaagaag cgcgccgaac aagtggcgat gctgctgcag ggcgcgagtc
     1681 tgactgcacc ctcctacaag gtcgcctggc gtgacgaggc cgccagcgcc agcggcgtcg
     1741 acgaccatct gtcccgtcgc gtcgtcatca cggtgcgtcc gcttcaatct cggcgttgac
     1801 gacgctcagt tcgggctcgg cgggagcggc cggttgtact gaatcgggca gcctggctgg
     1861 aagaactgcg cctcagcggc acggcggcag aatgcctgcg cgttgcgaac ctgtctctcg
     1921 atggccgaga cgacttcagc gagtggaaac ggcttctggg cgtgcacggc cagattcgtg
     1981 tcgaccttga ttttgcggta ctcaatgttg ttcatcaggc tgtcgcccca ccagttgaag
     2041 tcccagtcct gtgtcgtgag gtcggcctcg atgagcaggt tcgatgcggg gacgtggacg
     2101 atcacgccat ccgccatgtg atagttgccg acgatgtggt agatgtcgat ctcgttggtc
     2161 gagtccttca gctcgagatg atcgtcgacc gggatgaatt tcaacggctt cgggctgcgc
     2221 ccgagggcat ccgggaacaa cttggcggga cgcgccacca tttcgcggaa gatgccctcg
     2281 tttcctcgac gagagatgag cgtcaacccc tgagccacgg cagcgcgaag gccgacgctg
     2341 tggtcgaaat ggtgatgcga ttggatgacc tgcgtgacgc gcttgccggg cacgagggca
     2401 ttgacgacct ggaggatcgc ctggagtgcg gcatccggac ggttggcctc gaccatcgtg
     2461 atgtggtcgt cgaattcgaa gaacgtgttg ccgttcatgt accacacgtg gtcggcaacc
     2521 ttcatcggct tcaggttggc gttgcctccc gggggcgcgc cctgtggtgc cgccgagcgc
     2581 actgatgccg gcgcgctcag atcgtcaatc ggagcgtcga cgatgttgcg atcgacgtag
     2641 agcttgctct gcacgacgtt gcgaaagtcg atggtggtgg cgaagccggt gggcagttga
     2701 atgcccttct ccggcacgta gccggcaaat gccgtgcgat aggtgaggtc accgagattg
     2761 gcattcgggc cgacgtaact gacccaggcg gggagacttg ttgtcgcatc caccgccaac
     2821 gtcagggtgt cgccctgacg cacggtgacg tcgacgagct gcagattccc ctgcttgcgg
     2881 agattcgaca gcttgctggt cgcatcgagc gctgcgcgca cgatcgtgat cggatgggcc
     2941 agcagctcaa cacgtcgctg gcggaccgcc gctgcccctg cgagttgagg ctgcggcgtc
     3001 cctgattgag gggccgcgag gaatccgccg ccgaagttgt aggcgacgtc gccgtccagc
     3061 gtctcattcg tccgattgag cccgagctgc ccgatcgtcg aggcaaagac gaaatccatc
     3121 ttcaggcgct gctgcacgcg cgtgcgccag tgctcgagat cgatcgttcg cgtgtactcg
     3181 agcactgtct gccacttctg gggggcggca ggatctccgg tgatattccc gccgccgttg
     3241 aagtatgcga gctcaccgta gccgacgacc tgcagcgtct tgagcgccat cacgcggtcg
     3301 cggccgccga gcgcggcggc ggcgcgtgcc gcgagctgcc gttcgggagc cgcagactgg
     3361 ccagcgagcc gaatggcgag cgccgtggag atcgctgcca ccagtgaaag aacgacgacc
     3421 ctgttgtaca tcgcaactcc ctcttcgtct agcgcgacaa gcttaacggc agtcgttgat
     3481 cgtgcgcgat ccaatccgta gtcatcgagt tggccgatag caggtatcgt attcggcgat
     3541 acacgctgag cgtcactctt gtgcgagtct ctcctatatg cagaaccttg cgtggccaca
     3601 cattcacaga cgggcgaggg cgttcctggt gatcgtcgtc gttgttggag catccggatc
     3661 gtcaggcgct cagtggttca aggtcccgag cccgagagcg ccgcgcacag cgactggcga
     3721 cgtcgatctg acggcggcgc ccccgcggct ggcgaatgga agaccagacc tgtctggcgt
     3781 gtggatgacg gcggagcccc tctgtgtcat ccgggggaca gcaccgatca gcgagctgct
     3841 gaaaatgaac cctccgtccc gaaagtgccc gcctcgtacg gccagcttct cgcggcaaag
     3901 tatcaacatc ggcatcgaca tgcccggcgg gctgccgtac cagccatggc tcgccacact
     3961 cgttgacgag cgcacggcaa accaggcggt cgacgacccg cacatccgat gcctgcccga
     4021 cttctttctc cgagcgtacg ggctccctca ctatctgaaa ttcgtccaga cgccggatct
     4081 gctggtgatg ctgaacgagt acaacggcac ctaccgtcag gtctttacgg acggccgcca
     4141 gctgccggaa gccccgaatc cgtcgtggca gggctattcg acggctacat gggccgacga
     4201 cacgctggtc gtggacagca tcggatttcg cgacgacctg tggatcgact gggacggcag
     4261 catggtcacg gggaccgcaa aggtccgtga gcgaatccgg cggcctgcct tcggacacct
     4321 cgagatcgaa gtgacggtgg acgatcccaa ggcctatacg aagccctggt cggtgacctt
     4381 gcgtcagcag tttgccgcgg acaccgagct cattgatgaa atctgtgccg agggcgaacg
     4441 gttcgtggag actctcaagt aacgaacggc tcagagcgcg cggcgtgcga gggcgatccg
     4501 gctgcatccg tccatgacgt cgaggaactt ctgatactcg gctcggccaa cgacgaaggg
     4561 attcgcgtcg ttgcgcccgc gcgaggagag ccgcgtgagc caaccctgaa acgggaccat
     4621 cagcggatgg ttctgcagcc acgcatcgac gccggcgtcc accgtcgcct gcctgaagtg
     4681 actgactgag gcgatgtagg tcttcatcgc ctcgtcgttc agcagtcccg gcgtcagcca
     4741 cgatccgccg aagagcgcgg ccacgtgccg ctcgcctcga tcgaccactg gaatcacgaa
     4801 gcccatcgag ccgggcgtgt ggcctggaac ggccacggcg cgcacgctga cgtttccgag
     4861 cgtgactgac tctccatccc gcagctcgcc gtcgcgcttg ggcggcgcgg cgggcggcgg
     4921 cgctcccggg ccgcggccgc gcggcggcgt ctccatcaac gtccagtccg ccgccgacac
     4981 atagaccttc gcgccgaact tctgctgaaa atacgcagag ccgccaaagt gatcggcatg
     5041 cccgtggccg accagaatca ctttgacctg agccgggtcg aggccgagct tcgcgaatcc
     5101 tggcacgagg tccgactcga cctggttggc gcccaacgcg tcgatcatca tcaggcccgc
     5161 cgacgtctgg atcacgtagg cgactgtgcc aacgttgccg atcgcgtaca ggttgtcgaa
     5221 gaccttcgcg ggctgaatga ccggatcgtc cgctcgattc gcgcgcggcg cctcgcacca
     5281 gaagtgcacg gcgtcggccc agcgcgggcc aaccgacgtc ttgagccgat cgagctgctc
     5341 gatgatggct ggggaatcag gcttcgctgg tgctggcggc tggctctgct gcgcggccag
     5401 acgaaccgcc gcggcgcaca tcagcacgat gactgcgcag cgtgtcgaca tcatcatcgc
     5461 ggccctcctt ttatcgccga atggttcgat agacggcctc gacgaacatg tccgtcgtcc
     5521 acggaccggt cgtcgctcca taccgcgtat cgtaatccgc cgtcggccgc gccgccttca
     5581 cctgttcgag cgtgccaccg gctcgaacga gcgcgcgaat gcgatcgcga acgatcacca
     5641 ccatgtcgcg gtattcgacg acctcgtgct cgtccgagag atacccatgg cccgggacga
     5701 ccagcgtgcc accctggccc tgatgttcga acacggtccg ttccagaatc tggttgagcg
     5761 ccctgatctc gccctcgacg gtgccgccgt cggctacgtc gatgaacggg tactgcgttg
     5821 tcgtgaagat gttgcccgtg acgatcacat cggcgcgccg gaagtgtacc aggctgtcgc
     5881 cgtcggtgat cgcgttcggc tgccacagca tctcgatcgc gtcatcgttg tgaaacgtgc
     5941 ggcgcctctc ttcgaggaac gtgtcggtcg gcagcgcatc ggccgggcgc ttcgccgcgt
     6001 tcaagcggtt ctgcacgttc tgatgcgcca tgatcgtcgc gccgacgccg gcatcgcgga
     6061 attgcagcga gaagaacgtc ccgcgaacac tcgggtcggc cccggccttg cgcagcgctt
     6121 cgttgccgcc ggtgcgatcg ggaaggaaac tggtattggc gatgaactgc accggcttgt
     6181 cgctcagcgt ccggatggca gcgagcgtcc tggccgcccg cgcgccggtg ccggtgtcga
     6241 cgacgaacac gccttcgttg ccgacctgcg ccacgatgtt gccgccgtca ccaacgagca
     6301 tgtagacatt cccgcgcacc ggcaggatgt gaatgtcacc gtcggcaggc tccacaggta
     6361 aggccttgct cgtcgcctgc ggaccgcgtg acgggcgctc gagcgacgag tcgtctttcg
     6421 ccgcggccag catggacacg atctcgggct ggatgctcgc cgcgccgccg aggtgtccca
     6481 ccgccgcgag cgtgtacttg gtccgatact ccttgacgaa cgggttctga ccgaacagat
     6541 agctcggcac gtcgtccggc gctcggccga ggacctgttc gccatcgtca cacggaaaca
     6601 gccagttctg atgatcggcg ggctgacgga agaagtcggt ggtcgtgacc agcggctcct
     6661 cgagatagac cggatccgtg acgaccaccg tgtgcgtgag ccgatcgcca tggcggacga
     6721 agaactcgac gagcatggcc tgatcgctct ccggcgtgcc gtttcgtcga taccatcctt
     6781 gcttcagatg cgtcgtctgt acttccagtg cgttgccgac gaagcgaccg gtcgaaaacc
     6841 ccatccacga gtgcggggcg tacg
//
LOCUS       DBNGSH010000540         1907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2769119, whole genome shotgun sequence.
ACCESSION   DBNGSH010000540 DBNGSH010000000
VERSION     DBNGSH010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2769119"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="AC16CY_12400"
     CDS             complement(<1..325)
                     /locus_tag="AC16CY_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354639.1"
                     /translation="MSLSAPLVARAQVPHATFERGDLILSLESGPILWRLPDGTPRGT
                     LVSRVAGTGEGMAFDSSGRLFVARWRTDSFGITHDAVEVFNTFGVSSGEAGGGYNCGP
                     HAIVFD"
     gene            530..1903
                     /locus_tag="AC16CY_12405"
     CDS             530..1903
                     /locus_tag="AC16CY_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3354640.1"
                     /translation="MISRRSLLQAALAPLALPGVGHAQARVSAKAKPLPAGAVTHDWA
                     SFLGPTHNAVSTETKLRRALPPTLLWEYTKGAGYSSPAIAGGRLVFIHRVGDEEIVEC
                     LDAESGSKHWQFRYATAYEDRYGYNNGPRSSPVIDGDLVYTVGAESKLHCLELGSGKV
                     SWKRDLRGEYRRPRDFFGTASTPLVEGGLLVINVGAAGGPCVVGLDKATGKEVWRAGN
                     KWGPSYATPVPAVVHGQRRIFVFAGGESDPPVGGLMSIDPATGKVDFEFSWRSRAFES
                     ASAACPVVFDNKVFITASYRTGGALVEIQPDFTHKVLWTTQEFALHFNTPIYRGGYLY
                     GFDGRNEPDASLACLDAATGKIVWRVSPEWMDTLQGRELSLSTFRGSLLAADGHFLGL
                     GELGHLLWMDLSPKGYKELSRAWLFPARESWTPLALSRGLLYVMQNAVDVVRATRPRV
                     LCYDLRA"
BASE COUNT          342 a          621 c          626 g          318 t
ORIGIN      
        1 catcgaagac aatcgcatga ggaccgcagt tgtagccgcc gccagcctcg cccgacgaga
       61 cgccaaaggt gttgaacacc tccacggcgt cgtgggtaat gccgaaggaa tcggtgcgcc
      121 agcgggccac gaacaggcgg ccgctcgagt cgaatgccat cccctcaccc gtaccggcta
      181 ccctggaaac aagggtgcct cgcggcgtcc cgtctggaag acgccacagg atcggccccg
      241 actcgagaga caggatgagg tcacctcgct cgaacgtcgc gtggggcacc tgggcgcgcg
      301 cgacgagagg ggctgagagg ctcactatca agagaatgag tgaccacctg gccatggaat
      361 cagtgccttt cgtgcgccgt tctttgaggc gcaatgcccg atggtaggga ctcgggctgg
      421 agagcgccat caggtgattc acccctgtag gacagaaaaa agcccggtga ggtctggtac
      481 gtaactcacc gaggccgtca gaagacgtgc gacactctca gaggctctga tgatttcccg
      541 ccgctcgctg cttcaggctg ccctcgcgcc gctggcgctt ccgggcgtcg gccacgcgca
      601 ggcccgtgtg tccgccaagg cgaagccgct gccggccgga gcggtcacac acgactgggc
      661 ctcgttcctg gggccgaccc ataacgccgt ctccaccgaa acaaagcttc gccgcgcgct
      721 tccgccgact ctcctgtggg aatacacgaa aggcgccggc tattcctctc ccgcaatcgc
      781 cggcggacgg ctggtgttca tccaccgggt cggggacgag gagatcgtcg agtgtctcga
      841 tgcagagagc ggatcgaagc actggcagtt ccgctacgcg accgcgtacg aggatcgcta
      901 cgggtacaac aacgggcctc gatcgagtcc ggtcatcgac ggagatctcg tgtacaccgt
      961 cggtgccgag agcaagcttc attgcctcga gcttggttcg ggcaaggtgt cctggaaacg
     1021 ggaccttcgt ggcgaatacc gccggccgcg cgacttcttc gggaccgcat cgacaccgct
     1081 cgtggaaggc gggctgctcg tcatcaacgt cggcgccgcc ggcggcccgt gcgtcgttgg
     1141 actggacaag gcaaccggaa aggaagtctg gcgcgcgggc aacaaatggg ggcccagcta
     1201 cgcgacgccg gttcccgcgg tcgtccacgg ccagcgccgg atcttcgtct tcgccggcgg
     1261 tgaatcggat ccccccgtcg gcgggctgat gtcgatcgac ccggcgacag gcaaagtgga
     1321 cttcgagttc tcctggcgca gtcgggcctt cgaatccgcc agcgccgcgt gcccggtcgt
     1381 gttcgacaac aaggtcttca tcaccgcgag ctatcgcacg ggaggcgcgc tcgtcgaaat
     1441 ccagccggac ttcacgcaca aggtcctctg gacgacgcag gaattcgccc tgcacttcaa
     1501 cacgccgatc tacaggggcg gctacttgta cggattcgac ggccggaacg agccggacgc
     1561 ctcgctggcg tgcctggacg ccgccacggg caaaatcgtc tggcgcgtga gtcccgagtg
     1621 gatggacacg ctccagggac gcgagttgtc gctgagcacc tttcgcgggt cgctcctcgc
     1681 ggcggacggc cacttcctcg gtctcggcga actcggacat ctgctgtgga tggacctgtc
     1741 gcccaaggga tacaaagagc tgtcgcgcgc gtggttgttc ccggcccgcg agtcgtggac
     1801 cccgctcgcc ctcagccgcg ggctgttgta cgtgatgcag aatgcggtcg acgtcgtgcg
     1861 agccacgcgt ccgcgcgtgc tgtgctacga cttacgcgca tgaaagg
//
LOCUS       DBNGSH010000541         5404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_221875, whole genome shotgun sequence.
ACCESSION   DBNGSH010000541 DBNGSH010000000
VERSION     DBNGSH010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_221875"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..311)
                     /locus_tag="AC16CY_12410"
     CDS             complement(81..311)
                     /locus_tag="AC16CY_12410"
                     /inference="COORDINATES: protein motif:HMM:NF018404.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HIR3354641.1"
                     /translation="MFWPFGLACSFCHRSNDEVAKLVAGPRALLVGPPVYICDACVAI
                     AAKIMEQPVMPAPRPQAAGWRRWFSRTQVASS"
     gene            complement(347..1195)
                     /locus_tag="AC16CY_12415"
     CDS             complement(347..1195)
                     /locus_tag="AC16CY_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3354642.1"
                     /translation="MNARLFAVLAVIFGTALAAAQGPAFEVVSVKPQPQRPVRGARAA
                     PNRYYQPDTVLRSVIEFAYDLPSFRLENGPGWIESDRWAIDARAAVPARTSEMRLMVR
                     RLLADRFKFTAHVETRELPIYELYLAREDGKIGPNARPAALDCAPYLDGVRSVQELKD
                     TAAKDCSGSGIVMAGIGASGVVAVSHTTLRGVPMSALTTHLERALRRGVRDKTGVSGP
                     LDLELTYATDNPWPERPSLAFLAQLKESPPLFTALREQLGLELRASRGPVDVLVIDSV
                     ERPTPD"
     gene            complement(1266..2045)
                     /locus_tag="AC16CY_12420"
     CDS             complement(1266..2045)
                     /locus_tag="AC16CY_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3354643.1"
                     /translation="MVARIHVTLLALLAMTPTSPAQTVVSKGNVLRAAYLATNPAHAV
                     RDPATGETRGVAVDLARELGRSVGVGFALMPLENPQRVIDAVRNGDADIGFVAYNPER
                     AGPVEFSKPYLLVQQTFLVRADSRLRSVADADRAAVKLGATRNDSIALYLRRTLKQAL
                     LVEFDDSSRDAVHGMLREGTLDAFGANRQRLTDMQRGAADLRLLPDDLYGVEQTIIVP
                     SGRRDALDRVNRFIDDVRASGFLQTSIDRSGVVGIAVAPAR"
     gene            complement(2057..3877)
                     /locus_tag="AC16CY_12425"
     CDS             complement(2057..3877)
                     /locus_tag="AC16CY_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3354644.1"
                     /translation="MKALASLLLVLASSVPAFAQAVSGRAVYERRCASCHERPADGRT
                     PGRESLQKMTSSRILRTLDFGAMMTIAYQLRRDEREAVAGFLGTSAGDPVPRPEAFCR
                     DRTVTIDTSVSPVWNGWSPSPDNARFVPAPLARLGPDEVPRLKLKWAFGFEGDISAFA
                     QPTVIGNQVFVGSAGGVVHALRADSGCLQWTFQAVGPIRSAIVAAQSEGKPVLLFGDL
                     TGWFYALDAATGKQIWRKRPDEHEAVRLSAPPVVHEGLAFVVDSSWEESRSLNPEYPC
                     CNFRGSVTALRVRDGSIAWKTYMIPRVAERTGKTPAGAETWGPSGAGIWSAPTLDLKR
                     RRLYVTTGNNYSLPASPMSDAVVALDVDTGRIAWSKQLLPEDVYNSGCSTTPRGPSCP
                     EGNGPDYDFGSPAILVATSGGRELLLAGQKSGIVWALDLARDGELMWEARVGQGGVNG
                     GVQWGMAADGEHVFAAVSDAVSTRTATERTLDPKLGGGLTALRIADGSKAWYAAPTPC
                     EASRARCSPAQSAALTAIPGVVFSGSLDGHVRAFSSRDGSILWDFDTARDYQTVNGVK
                     GAGGAIDGPGVVVVGGMVFVNSGYMRFGGLPGNVLLAFAP"
     gene            complement(3874..4308)
                     /locus_tag="AC16CY_12430"
     CDS             complement(3874..4308)
                     /locus_tag="AC16CY_12430"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3354645.1"
                     /translation="MMRVLVLSTLVVVGLSSAVWSQATQPAAPPPAPNPANFTGKVTP
                     HPTTDIRTLRYSFEPGARTNWHSHEGGQVIHIEQGNMLAQEAGGPVKEYVPRQTMTTA
                     PGVKHWHGALPNQPLTQVALSFGTTNWMEKVTDAQYSAGPRK"
     gene            complement(4448..>5404)
                     /locus_tag="AC16CY_12435"
     CDS             complement(4448..>5404)
                     /locus_tag="AC16CY_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3354646.1"
                     /translation="WKTGGGSIWVTPSYDPALNLTYWGVGNPGPDWNPDQRPGDNLYT
                     DSVVALDPDTGALKWHFQFSPNDPYDYDAVQIGVLADLNWRGMQTKAILWANRNGFFY
                     VLDRTNGRFLSGTPFVKVNWASGLDPNGRPIPTPQPEGQPTWPGIQGGTNWYSPSFSP
                     RTGLLYVSAWEGYASVFKKEKVEYVPGRNFLGGGGTPFTGVPGAPGVRVGRTSPLNTW
                     TDAAGNGAVIALDPQTGQRKWTFKQFDVTDSGILTTATDLLFTGGREGYFYALDARTG
                     AELWKASLGGQIVNGPISYAVDGKQYVATISGHSLVVFALRD"
BASE COUNT          912 a         1835 c         1785 g          872 t
ORIGIN      
        1 tcgtacctcg cgaagaagag ggttcggcgg gctggctccg tgtaacgctc gaacatggcg
       61 atcagcgggt cgatcaagca tcaggaactt gcgacctgcg tgcggctgaa ccatcgccgc
      121 cagccggcgg cctggggtct gggcgccggc atcacgggct gttccatgat ctttgcggcg
      181 atagcgacgc aggcatcaca gatgtagacg ggcggaccga cgagcagcgc acgcgggccg
      241 gcaacgagct tggcgacctc gtcgttgctc cggtggcaga acgagcaggc aagaccgaac
      301 ggccagaaca tggggcacct ccgcctcgaa gtttacgccc ttccgctcaa tccggcgtgg
      361 gccgctcgac ggagtcgatc acgaggacgt ccacaggacc tctgctggct ctgagctcca
      421 atccgagctg ttctcgaagc gccgtgaaca ggggcggact ctctttcagt tgcgcgagga
      481 aggcgagcga cggccgctca ggccatggat tgtcagtcgc atacgtgagc tccagatcca
      541 agggaccgct cacgcccgtc ttgtctctga ccccacgacg aagcgccctc tcgaggtggg
      601 tcgtcagggc agacatcgga acgccccgta acgtcgtgtg agaaacggcc acaacaccag
      661 aagctccgat gccggccata acgattccgc taccactaca gtccttcgcg gcggtgtcct
      721 tcaactcctg aaccgagcgc acaccgtcga gatacggggc gcagtcaagt gcggcgggcc
      781 tggcattcgg cccgatcttg ccatcctcgc gagccagata gagctcatag atcggcagct
      841 cgcgagtctc gacgtgagca gtgaacttga atcgatcggc caggaggcgg cgcaccatca
      901 gacgcatctc gctagtacgt gccggcacgg cggccctcgc gtcgatcgcc caccgatccg
      961 actcgatcca tcccgggcca ttctcgagtc ggaatgatgg caggtcgtag gcgaactcaa
     1021 tcacagaccg caggaccgtg tcgggctggt agtagcgatt gggggctgcg cgggctccgc
     1081 ggacaggccg ttggggctgc ggcttgacgg aaacgacctc gaacgcagga ccttgagctg
     1141 cggccagagc ggtaccgaat atgactgcga ggacggcgaa cagtcttgcg ttcatacgac
     1201 cttggtccgg ctgaagccag acactacgta ctcacggtcc ggctgaagcc ggacactacg
     1261 cctgatcacc gtgccggggc aacggcgatc ccgacgacgc cgcttcgatc gatcgatgtc
     1321 tgcaggaacc ccgacgcgcg cacgtcgtcg atgaagcgat tcacgcggtc gagcgcgtca
     1381 cgtctgccag atggcacgat gatcgtctgc tcgaccccgt acagatcgtc gggcagcagc
     1441 cggagatcgg cggcgccccg ttgcatgtcg gtgaggcgct gacgattggc gccgaaggcg
     1501 tcgagcgttc cctcgcggag catcccgtgc acagcgtcgc ggctggaatc atcgaactcg
     1561 acgagtagcg cctgcttgag cgtccgccga aggtacagcg cgatcgagtc gttgcgtgtc
     1621 gcgccgagct tcacggccgc gcgatcggcg tcggcaaccg accggagccg cgagtcggct
     1681 cgtaccagaa acgtctgctg gacgagcagg tagggcttgg agaactcgac cggcccggcg
     1741 cgctcggggt tgtaggcgac gaacccgatg tcagcgtcgc cgttgcgcac cgcgtcgatg
     1801 acgcgttgcg ggttctcgag cggcatcagc gcgaacccga cgcccacgct ccgtcccagt
     1861 tcccgcgcca gatcgacggc gacgccgcgc gtctcgccgg tcgcgggatc cctgacggcg
     1921 tgagcaggat tggtggcgag gtaggcggct cgcagcacat tccccttcga tacgacggtt
     1981 tgcgccgggc tcgtcggggt catcgccagg agcgcgagca gcgtgacgtg gatgcgcgca
     2041 accatctgcg gccaggtcag ggcgcgaacg cgagcagcac gttgcccgga agaccgccga
     2101 accgcatgta acccgagttc acgaagacca ttccccccac gacgaccacg ccgggaccgt
     2161 cgatggcgcc gcccgccccc ttcacgccgt tgaccgtctg gtagtcgcgg gccgtgtcga
     2221 aatcccagag gatcgatccg tcccggctcg aaaaggcgcg cacgtggccg tcgagagagc
     2281 ccgagaacac gactcccgga atcgccgtca gcgcggccga ctgcgcgggg ctgcagcgcg
     2341 ccctcgacgc ctcgcacggc gtcggcgccg cgtaccacgc cttgctgccg tcggcgatgc
     2401 gcagcgccgt gagtccgccg cccaacttcg gatccagcgt tctctcggtg gcggtcctcg
     2461 tcgagacggc atcggaaacc gcggcgaaca cgtgctcgcc gtcggcggcc attccccact
     2521 gcacgccgcc gttcacgccc ccctggccca cgcgcgcttc ccacatcagc tcgccgtccc
     2581 tggcgagatc gagcgcccag acgatgcccg acttctgccc ggcgagcagc agctcgcggc
     2641 cgccggacgt ggcaaccagg atcgccggcg acccgaagtc gtagtctggc ccgtttccct
     2701 cggggcaact gggtcccctc ggcgtcgtcg agcagccgga gttgtacacg tcttcgggca
     2761 gcagctgctt cgaccacgcg atgcggcccg tgtccacgtc gagtgccacg acggcgtcgc
     2821 tcatcggcga cgcgggcagc gagtagttgt tgccggtggt cacgtagaga cgccgccgct
     2881 tcagatcgag cgtcggcgcc gaccagatgc cggcgcccga tggtccccac gtctcggcgc
     2941 ccgccggggt cttgccggtg cgctccgcca cgcgcggaat catgtaggtc ttccacgcga
     3001 tgctgccatc gcgaacccgg agggcggtga cgcttccgcg gaagttgcag cacgggtatt
     3061 cgggattgag cgaccgcgac tcctcccacg aggagtccac cacgaaggcg agcccctcgt
     3121 gcacgaccgg aggcgcgctc aggcgcactg cctcgtgctc gtcgggccgc ttccgccaga
     3181 tctgctttcc ggttgccgca tcgagcgcgt agaaccatcc cgtgagatcg ccgaagagca
     3241 gcaccggctt cccctccgac tgcgccgcca cgatcgccga tcgaatcggg cccacggcct
     3301 gaaacgtcca ttgcaggcag ccgctgtcgg cgcgcagcgc gtgcacgact cccccggcgc
     3361 ttcccacgaa gacctggttg ccgatcaccg tcggctgagc gaacgcggag atgtcccctt
     3421 cgaatccgaa cgcccacttc agcttcagcc gcggcacctc gtcgggaccg agcctggcga
     3481 gtggggcggg cacgaaacgc gcgttgtctg gcgaagggct ccacccgttc cagacgggcg
     3541 agaccgaggt gtcgatcgtg acggtgcgat cccggcagaa ggcctccgga cgcggcaccg
     3601 gatcaccggc ggacgtgcca agaaagccgg caaccgcttc gcgctcgtcg cgccgaagct
     3661 gatacgcgat ggtcatcatt gcgccgaaat cgagcgtgcg caggatgcgc gaactcgtca
     3721 tcttctgcag cgactcccgc ccgggcgtcc ggccgtcggc gggccgctcg tggcagctcg
     3781 cgcagcgccg ttcgtacacc gcacgccccg aaacggcctg cgcgaacgcc gggaccgacg
     3841 aggcgaggac cagaagaagg ctggcgagcg ctttcacttc cgggggcctg ccgagtactg
     3901 ggcgtcggtc accttctcca tccagttggt ggtgccgaag ctcagcgcca cctgcgtcag
     3961 cggctggttc ggcagcgcgc catgccagtg cttgacgcca ggggcggtcg tcatggtctg
     4021 gcggggaacg tattccttaa cgggtccgcc ggcttcctgc gcgagcatgt tgccctgctc
     4081 gatgtggatg acctgaccgc cttcgtggct gtgccagttg gtgcgcgccc cgggttcgaa
     4141 gctgtagcgc agcgtccgga tgtcggtcgt cggatgcggc gtgactttgc ccgtgaagtt
     4201 cgcgggattc ggggccggcg gcggcgcggc gggttgcgtg gcctgactcc agaccgcgga
     4261 tgaaagaccg acgacgacga gcgtcgagag gacgagcaca cgcatcatga gaacaccacc
     4321 ttcgatgtac acctgcacgc gcgagctgaa gctcgcgctc tacgcgttca cgccactgaa
     4381 gctcgcgctc tacgtgtcac gccaccaacg tcgtagagcg caggcttcag cctgcgcgga
     4441 tctccgttca atcgcgaagc gcgaaaacaa ccagcgagtg tcccgaaata gtcgcgacgt
     4501 actgtttccc gtccaccgcg tagctgatcg gcccgttcac gatctggccg ccgaggctcg
     4561 ccttccacag ctcggcgccc gttctggcat cgagcgcgta gaagtagcct tcgcgcccac
     4621 ccgtgaacag cagatccgtg gccgtcgtca gaatgccgct gtcggtgacg tcgaactgtt
     4681 tgaaggtcca cttccgctgg ccggtctgcg gatcgagagc gatgacggcg ccgttgcctg
     4741 cggcatcggt ccaggtgttg aggggcgacg tgcgtccgac gcgcacgccg ggcgcgccag
     4801 gcactccggt aaagggcgtg ccacctccgc ccaggaagtt gcgcccgggc acgtactcca
     4861 ccttctcctt cttgaagacc gacgcatacc cttcccacgc cgagacgtac aacaaccccg
     4921 tgcgcggact gaatgacggc gagtaccagt tggtgccccc ctggatcccc ggccacgtcg
     4981 gctgcccttc cggctgcggc gtcgggatgg gcctgccgtt cgggtcgaga ccgctcgccc
     5041 agttgacctt gacgaaggga gtgccggaga ggaaccggcc gttcgtccgg tcgagcacgt
     5101 agaagaatcc gttccggttg gcccagagga tggccttcgt ctgcatccct ctccagttga
     5161 gatcggcgag cacaccgatc tggaccgcgt cgtagtcgta gggatcgttc ggcgagaact
     5221 ggaagtgcca cttcagagcg cccgtatcag ggtcgagcgc cacgaccgag tcggtgtaca
     5281 ggttgtcgcc cgggcgctga tcgggattcc agtccgggcc ggggttgccg acgccccagt
     5341 aggtgaggtt gagcgcggga tcgtacgagg gcgtcaccca gatcgacccg ccgccggtct
     5401 tcca
//
LOCUS       DBNGSH010000542         1570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2599422, whole genome shotgun sequence.
ACCESSION   DBNGSH010000542 DBNGSH010000000
VERSION     DBNGSH010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2599422"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="AC16CY_12440"
     CDS             complement(<1..709)
                     /locus_tag="AC16CY_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3354647.1"
                     /translation="MKSAAALAVLLACAAAIASAQSPSSAFARGNSLQSWQNPGLKSV
                     LAKCKTPPAPFAIPGAGAANAKPAPPPEPPAPPASTAIPGVIAAGQTWKVVWSWEGNN
                     ADGIIAGDEGTMLFANNDASNVMKLDPATGLASIIHTDTNTGGALSRSRNGALFLASR
                     GLNAGILQLEPQRKSFATSFRGEPLDCAGGVVIAFDVGPDGALTRQREFGKLRGGQGG
                     DGSAVDAQGRLYVSTGAS"
     gene            complement(729..>1570)
                     /locus_tag="AC16CY_12445"
     CDS             complement(729..>1570)
                     /locus_tag="AC16CY_12445"
                     /inference="COORDINATES: protein motif:HMM:NF024151.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050525 - cutinase activity [Evidence
                     IEA];
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="poly(ethylene terephthalate) hydrolase family
                     protein"
                     /protein_id="HIR3354648.1"
                     /translation="GVTREMIASALPVEGAPLAEPGPYAVTSEAAFGSPGHIVFRPTN
                     LDAFPKKDTLPVLVWGNGGCAINSRRYSGFFTTIASHGFLVLSTVAQEGAQQRQQNAD
                     DLRAAIDWAEKENARAGSPLNGKIAVDKVAVMGQSCGGFLSIGLGADPRVDTIGVFNS
                     GVQAAGSGGGRGGVSTDALAKLHGPVLFINGHERDFMMAASLANYEAIDRLPAFYGAR
                     HNAGHTATVDHPGGGEFANVASNWVRWTFKGDKRAGAMFVGKSCSLCTNPNWDVKSKR
                     LPE"
BASE COUNT          233 a          543 c          538 g          256 t
ORIGIN      
        1 ccgatgcccc ggtggacacg tacagacgcc cctgcgcatc gacggccgac ccgtctccgc
       61 cctggccgcc gcgcagcttt ccgaactccc gctgcctcgt cagcgcgccg tcgggcccga
      121 cgtcgaaggc gatgaccacc ccgccggcgc agtcgagcgg ctcgccgcga aaggaggtcg
      181 caaacgattt gcgctgcggc tcgagctgca ggatgccggc gttcagcccc ctcgacgcaa
      241 gaaacagcgc gccattcctg ctgcgcgaga gggcgccgcc ggtgttggta tccgtgtgga
      301 tgatcgacgc gaggcctgtc gcggggtcga gcttcatgac gttgctcgcg tcgttgttgg
      361 cgaacagcat cgtgccctcg tcaccggcga tgatgccgtc ggcgttgttg ccctcccacg
      421 accacacgac cttccacgtc tgcccagcgg cgatcacgcc gggaatggct gttgacgcgg
      481 gcggcgccgg cggctctggc ggcggcgccg gcttggcgtt cgccgccccg gcgcccggaa
      541 tcgcgaaggg cgccggaggg gtcttgcact tggcgaggac gctcttcagt ccgggattct
      601 gccaggactg caagctgttg ccgcgggcaa aagcagaaga cggactctga gcagaggcga
      661 tggctgccgc gcaggcgagg agcacggcga gcgctgccgc tgatttcatg accgacactc
      721 cgagcgcctt actcgggaag gcgcttcgat ttcacgtccc agttgggatt ggtgcagagg
      781 ctgcagctct tcccgacgaa catcgccccg gcgcgcttgt ctcccttgaa cgtccaccgg
      841 acccagttcg acgcgacgtt ggcgaactcg ccgccacccg ggtgatcgac tgtcgccgtg
      901 tgtccggcgt tgtgccgcgc gccgtagaac gcgggcaggc gatcgatcgc ctcgtaattc
      961 gccagcgacg cggccatcat gaaatcgcgc tcgtggccgt tgatgaacag gacgggaccg
     1021 tgcagcttgg cgagcgcatc cgttgaaaca ccgcctcggc cgccgccgga gcctgccgct
     1081 tgcacgcccg agttgaacac gccgatcgta tcgacgcgcg ggtcggcgcc gaggccgatc
     1141 gacaggaagc cgccgcacga ctgtcccatg acggccacct tgtccacggc gatcttgccg
     1201 ttcagcggcg agcccgcgcg cgcgttctcc ttttcggccc agtcgatcgc cgcgcgcagg
     1261 tcgtcggcgt tctgctgacg ctgctgcgcg ccttcctggg ccaccgtgct gagcacgagg
     1321 aagccgtgcg aggcgattgt cgtgaaaaag cccgagtagc gtctgctgtt gatcgcgcag
     1381 ccgccgttgc cccacacgag cacgggcagc gtgtccttct tcgggaacgc gtcgaggttc
     1441 gtcgggcgga acacgatatg gccgggcgag ccgaaggccg cttcggaggt gacggcgtac
     1501 ggcccgggtt cggcgagcgg cgcgccctcg acgggcagcg ccgacgcgat catctcgcgc
     1561 gtgacgcccg
//
LOCUS       DBNGSH010000543         2704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3618382, whole genome shotgun sequence.
ACCESSION   DBNGSH010000543 DBNGSH010000000
VERSION     DBNGSH010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3618382"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="AC16CY_12450"
     CDS             complement(<1..460)
                     /locus_tag="AC16CY_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3354649.1"
                     /translation="MLALLCAVTVVALGAQGRGAPRPECVNPKIPAAECNIGATAIQW
                     ADPPLGDGPFLIESALPQHRNLRVVVMARLEQPWSIAFLPDGGIFVTERGGRLRIIRN
                     GVLDPNPVAGVPAVRAGGLQGLMDVVLHPRFAENRWVYLAYHKPVTVPGQT"
     gene            complement(676..1566)
                     /locus_tag="AC16CY_12455"
     CDS             complement(676..1566)
                     /locus_tag="AC16CY_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3354650.1"
                     /translation="MQRQTTADFHPEVLRLFDRYVHGLIDRRGFLEGAGKYAAVGTTA
                     VGLLEALSPKYAEAQQIAKDDKRIKAEYAEYQSPQGHGKMRGYLVRPANAKGRLPGIV
                     VVHENRGLNPHTEDVARRAALENFVAFAPDALTPLGGYPGTDEAGVEMQRKLEQPKIQ
                     EDFLAAVNWLRAHQETTGRVGIVGFCFGGGISNAMAVRVPELGAAVPFYGGQPRAEDV
                     PKINAPLMLQYAEMDERINAGWPAYEAALKANNKRYTVHIYPGTQHGFLNDTTPRYNE
                     AAAKLAWQRTIEFFNKNLRT"
     gene            complement(1643..>2704)
                     /locus_tag="AC16CY_12460"
     CDS             complement(1643..>2704)
                     /locus_tag="AC16CY_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354651.1"
                     /translation="VRPAPGGATFTTSAANQPPLEQTVIPDGANHPIVQQDCRGTQQS
                     EWSRDGLMLFTRATLTCAEGQETRHVSGLSRVGPGSWVDVQAVDVDGQESIRVRRYRR
                     ASDSARTTRRARPLSIEEVTEASRKVPSGVLEATLVETYSTFWLSGQLLRSLQKDGVP
                     PNVTDLMIALSYPDRFDVGRRGSDDRAAGTTTTTIDEGLAAVGWGVGLPPPWYADPFY
                     SGYYPYFYSPYYYPYVDHYYYPPYYGSSVVVIEDDGKLGTGAARAVNGVGYTRVRPRN
                     DGGGQRPEPRGAFVSSPSSGGSSSSASSGPSSSGSSSSGSSSSSGSAPSSSGGSTVSS
                     QGHSSGGSSGDTGRTAQPR"
BASE COUNT          440 a          889 c          892 g          483 t
ORIGIN      
        1 tcgtctggcc cggcaccgtg acgggcttgt ggtacgccag gtacacccag cggttttccg
       61 cgaagcgggg gtgcagcacc acgtccatca gtccctgcag tccccctgcg cgcaccgcgg
      121 gaacgccggc gacgggattg gggtcgagca cgccgttccg gatgatgcgg agccggccgc
      181 cgcgctcggt gacgaaaata ccgccgtcag ggagaaacgc gatcgaccat ggctgctcaa
      241 gccgcgccat gacgacgact cggagattgc gatgctgggg cagcgccgat tcgatgagga
      301 acggcccgtc gccgagaggc gggtcggccc actgaatcgc ggttgcgccg atgttgcatt
      361 cggcggcggg aatcttcggg ttgacacatt cagggcgcgg cgcgccacgc ccctgggcgc
      421 ccagggcgac gactgtgacg gcgcacagca acgcgagcac gctcgccaat cctttccatc
      481 ctcgatacat cttcgactcc ctatgcccac tgcccgtaga ggcgggtctt cagacccgcc
      541 ttggattgcc ccgtgttcaa cggcgggtct gaagacccgc cgctacaccg tcgtggaagt
      601 gttcaacggc gggtctgcct tcgaccacgc ccaggccagg aaagacccgc cgctacaccg
      661 tcgtggaaac gactgctacg tgcgcaggtt tttgttgaag aactcgatcg ttcgctgcca
      721 cgccagcttg gcggccgcct cgttgtagcg cggagtcgtg tcgttcagga acccgtgctg
      781 cgtgcccggg tatatgtgga ccgtgtagcg cttgttgttc gccttgagcg ccgcttcata
      841 cgcgggccag ccggcgttga tgcgctcgtc catctccgcg tactggagca tcaacggagc
      901 gttgatcttc gggacgtcct cggccctggg ctgtcctcca tagaagggga ctgcggctcc
      961 cagttccggg acgcgcacgg ccatcgcatt ggagatgccg cccccgaaac agaacccgac
     1021 gatacctacg cgtcccgtgg tctcctgatg ggctcgcagc cagttgacgg ctgccaggaa
     1081 atcctcctgg atctttggct gctccagctt gcgctgcatc tcgacgccgg cttcgtcggt
     1141 tccggggtat ccgccaaggg gcgtgagcgc atccggcgcg aacgcgacga aattctcgag
     1201 cgctgcgcgg cgcgccacgt cctcggtgtg tggattcagg ccccggttct cgtgaacgac
     1261 gacgatgccg ggcaatctgc ccttcgcgtt ggcgggacgc acgagatagc cccgcatctt
     1321 gccgtggcct tgtggcgact ggtactccgc gtactcggcc ttgattcgct tgtcgtcctt
     1381 ggcgatctgc tgtgcctcgg cgtacttggg gctgagcgct tccagcagcc caacagccgt
     1441 cgtcccgaca gctgcgtact tgccggcgcc ctccaggaac cctcggcgat cgatgaggcc
     1501 gtgaacgtaa cggtcgaaga gtctcaggac ttccgggtgg aagtcggcag tggtctgtcg
     1561 ttgcatggcg gcgctcctct acactgcagc tcgctcgtgc tggcacgagc gagtcctggg
     1621 ttaaagggcc gccagaatac cactatctcg gttgggccgt ccgtcccgta tccccgctcg
     1681 aaccgccgct cgaatgtccc tggctcgaca cggtcgagcc cccggatgag gatggcgccg
     1741 agccggacga agacgacgaa ccggacgacg atgagccgga cgaggacgga ccagacgatg
     1801 ccgacgacga gctcccgccc gaggatggtg agctgacgaa ggcacctctc ggctccggtc
     1861 gctggccgcc gccgtcgtta cgaggccgca cgcgcgtgta tccaacgccg ttcaccgcac
     1921 gcgcagctcc ggtgcccagc tttccgtcgt cttcaatcac gacgacagac gagccgtagt
     1981 agggcgggta gtagtaatgg tcgacgtagg ggtagtagta cggcgaatag aagtacgggt
     2041 agtacccgga gtagaaagga tctgcgtacc agggcggcgg aaggccgact ccccagccca
     2101 cggctgcaag accctcatcg atcgtcgtcg ttgtcgtccc tgcggcgcga tcgtccgatc
     2161 ccctgcggcc cacgtcgaag cggtccggat aggacagcgc gatcatcagg tcggtcacat
     2221 tcggcggcac gccgtccttc tgaagagagc ggagcagctg cccggacagc cagaacgtgg
     2281 aataggtctc gacgagcgtg gcttcgagca cgccggacgg aaccttacgg ctggcctccg
     2341 tcacttcctc gatcgacagg ggacgtgccc tgcgggtcgt tcgagcgctg tccgaggcgc
     2401 ggcggtagcg ccggacgcga atcgattcct gcccgtccac gtccacagcc tggacgtcta
     2461 cccacgaccc ggggccgact cgtgaaaggc ccgagacgtg gcgggtttcc tgaccctcgg
     2521 cgcacgtgag cgtcgcccgt gtgaagagca tcaagccgtc ccgggaccat tcgctttgct
     2581 gcgtgccccg gcaatcctgc tggacgattg gatggttggc gccgtccggg atgaccgtct
     2641 gctcgagggg cggctggttt gcggcgctgg tcgtgaacgt cgcgccaccc ggggccggcc
     2701 gcac
//
LOCUS       DBNGSH010000544         4643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_901985, whole genome shotgun sequence.
ACCESSION   DBNGSH010000544 DBNGSH010000000
VERSION     DBNGSH010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_901985"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="AC16CY_12465"
     CDS             complement(<1..1019)
                     /locus_tag="AC16CY_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354652.1"
                     /translation="MLTLERIWQDLRHGLRVFVKAPAFAAIAVVSIAFGTGANVAIFS
                     AVDALLLRPPPVERPDELITVGTRVDRGLGSMLRMSHADYVDVRTRARTFEGLLAFGF
                     RWVGFSAAPGQAARVTVAALVSANYFQLLGIEPQLGRTFLPDEDRVPGRDAVVILSDD
                     VWQQEFARDPAIVGRIVRISGIDFTVVGVAPRSFTGLDLYNRHGVYIPLAMWNQVMNS
                     PLANPLEDRSLRVLTVDGRLRSGVSLVTARAELSAIGDDLSRLHPATNTGRAITAQTE
                     LQMRFERRPLDTHTLVLLSALSLAVLCVACANVAGLLASRAPERAREIALRLSIGASR
                     PRIITG"
     gene            complement(1073..3880)
                     /locus_tag="AC16CY_12470"
     CDS             complement(1073..3880)
                     /locus_tag="AC16CY_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3354653.1"
                     /translation="MSISRLLRVLRQRLRALFRRTSVDEELNRELAFHLEQLVQEFAE
                     RGMPLEDARHAARRAIGNIPSLEEQCRDHRSVTWIHDVRRDVVYGVRMLRKNPGFTLV
                     AVLSLAMGIGANTAIISVMDAVFRDNLPIPDDERVVVARTFPLDDPRQETHAPISDYY
                     EWRDASHSFDLMGVSLGHQADFGADAAGTPAERVNGQLATAEALAVLGVQPIIGRVFT
                     AAEAHIEAPERLIVISHRLWQRRFGGRAGILGEQVRLDRVNWTIVGVMPEGFRFPNQG
                     ADYWIPLRLDRSQRQSPQRFYVVTARLKDGVTISQAQSDLDIIAARLARDDPDWHEGW
                     GVRVKPLREAMLGWSLAPLVTLGSAVALVLLVACANLAGLLLSRGLVRMPEMAMRTAL
                     GAGRSRIVRQLLTESILLSVAGGALGLLVAWGGIRSLVGMTPPPGGVEIVAVTLNLRS
                     LIVTALISIAAGILFGLAPALMGTRTGVTATLKESPIASVSGLPPRFRGALVATQIAV
                     TFVLLIGSGLLMKSFVQLVSRDLHFDPERLLTFEYHTPLGDYMQRRGSVGGLPYFAID
                     PPPSLALERVHRGLQALPGVESVAGASFPLLNSVVLPTMSVSVENLSELAASPGSAGL
                     SAPLAIGVGSVGAHLASDRRGLSAAYFLVTPGFFTSIKGRVVEGRDFAESDTSSSEWV
                     AIVNETAAARFWPGESPLDQRLRLIIVPEERPRRVIAVVRDIPPTRQSGPTPVIYTSY
                     LQQPLHYPQPGANMLGRMTFMVRTTGDDPMALLPAARRVVADVDPDRPLANVVTMEQQ
                     LASVVPRRGYLVVAMVAFAFMAMLLASIGIYGVISYSVTQRTREIGIRVALGAGARDV
                     VGLVGGRALVLIAIGLSAGLGGALALTRFLQSQLWGVTPTDPATFAAAILLLVVVALG
                     ACVFPLRRATGVDPTIALRCE"
     gene            complement(3941..4270)
                     /locus_tag="AC16CY_12475"
     CDS             complement(3941..4270)
                     /locus_tag="AC16CY_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3354654.1"
                     /translation="MPKPSDLLQGTLDLLILKTLVRESLHGWAISKRIQLLSNEVLAV
                     QQGSLYPALHRLEQQGWISAEWRDTEQGRSGKFYSLTRQGKRQLERELESWKRLSSAV
                     GLLLERA"
     gene            4322..>4643
                     /locus_tag="AC16CY_12480"
     CDS             4322..>4643
                     /locus_tag="AC16CY_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354655.1"
                     /translation="MIDTSSTPVAPLLRRGVLLLGAVVCAGAVLSAGDGRARLQMNAV
                     TGGTITPFAPAGKAGVVFFVATDCPVSNSYAPEIQRLCREYGPRGVGCSLIYEDVDVH
                     PTSAH"
BASE COUNT          805 a         1577 c         1578 g          683 t
ORIGIN      
        1 ccggtgatga tccgcggccg gctggctccg atcgagagcc ggagggcgat ctcgcgcgcg
       61 cgctccggcg cccggctggc gagcagcccg gccacgttcg cgcacgcgac gcagaggacc
      121 gcgagcgaga gggcgctcag cagcaccagc gtgtgtgtgt cgagagggcg ccgctcgaac
      181 cgcatctgca gttccgtctg ggccgtgatg gcccgccccg tgttcgtcgc cggatgcagg
      241 cgcgacaggt cgtcgccaat ggccgagagc tccgcgcgcg ccgtgaccag ggaaaccccg
      301 ctcctgagcc gcccgtccac cgtgagcacc ctgaggctcc gatcctcgag aggattcgcg
      361 agcggtgagt tcatcacctg gttccacatg gcgagcggga tgtacacacc gtgccggttg
      421 tacagatcga ggccggtgaa cgacctgggc gcgacaccga cgactgtgaa atcgatgccc
      481 gagatgcgga cgatccggcc gacgatggcc gggtcccggg cgaactcctg ctgccacacg
      541 tcgtcgctca gaatgacaac ggcatcgcgc cccggcacgc ggtcctcgtc aggtaagaac
      601 gtacgtccga gctgcggctc gatccccaga agctgaaagt agtttgcgct caccagggcg
      661 gcaaccgtga cgcgggccgc ctggccagga gcagccgaaa agcccaccca gcggaacccg
      721 aacgccagga ggccttcgaa cgtgcgtgcg cgggtgcgga cgtccacgta gtcggcgtgc
      781 gacatccgga gcatgctccc gagcccgcga tcgactcgcg tgccgaccgt gatgagctcg
      841 tcgggacgtt cgaccggcgg tggcctcagc agcagcgcgt ccacggcgct gaagatcgcg
      901 acgttggcgc ccgtgccgaa cgcgatggac acgacggcga tcgccgcaaa ggcgggcgcc
      961 ttgacgaaca cgcggagtcc gtggcgcagg tcctgccaga tccgctcgag ggtcagcatg
     1021 atgcgcaagc cacgcttctg cgtacgttag tgtccggctt tagccggacc cgttactcgc
     1081 atcgcaacgc gatcgtcggg tccacgccgg tggccctgcg gagggggaag acgcacgccc
     1141 cgagcgccac taccacgagc agcaggatcg ccgcggcgaa tgtggccgga tcggtcggcg
     1201 tgacgcccca gagctgggac tggaggaagc gagtcagcgc cagcgctcca cccaggcccg
     1261 ccgacagccc gatcgcgatc agcaccagcg cgcggccgcc gacgagccct acgacgtcgc
     1321 gcgcaccggc gcccagtgcc acgcggatgc cgatttcacg cgtgcgctgc gtcacggagt
     1381 aggagatgac gccgtagatg ccgatggacg cgagcagcat cgccatgaac gcgaacgcga
     1441 ccatcgcgac gaccagatac ccccgccgcg gtaccacgga ggccagttgc tgctccatcg
     1501 tcacgacatt ggccagtgga cggtccggat cgacgtcggc gaccacacgc cgcgcagcgg
     1561 gaagcagggc catcgggtcg tcgccggtcg tccgcaccat gaacgtcatc cggcccagca
     1621 tgttcgcgcc cggttgcggg tagtgcagcg gctgctgcag gtacgacgtg tagatcaccg
     1681 gcgtgggacc gctctgtcga gtcggaggaa tgtcccgcac gacggcgatc acccggcgcg
     1741 gacgctcctc gggcacgatg atcaatctga gccgctgatc gagagggctt tcgcccggcc
     1801 agaatcgggc cgccgcggtt tcgttgacga tggccaccca ctcggaggag gaggtatcgc
     1861 tctcggcgaa atcacgtcct tccacgacgc gccctttgat cgacgtgaag aaaccgggcg
     1921 tcaccaggaa gtaggccgca ctcagcccgc gccgatccga ggcgaggtga gccccgacgc
     1981 tccctacgcc gatggcgagg ggcgccgaca gtccggcact cccgggtgag gcggcgagct
     2041 cggacaggtt ctcgacgctg accgacatgg tgggcaggac cacgctgttc aggagaggga
     2101 aggacgcgcc ggccaccgac tccactccgg gcagggcctg cagtccccgg tgcacgcgtt
     2161 cgagcgcgag cgacggcgga ggatcgatcg cgaaatacgg cagcccgccc accgaacccc
     2221 ggcgctgcat gtaatcgccc aacggcgtgt gatactcgaa cgtcagcagc cgctcgggat
     2281 cgaagtgcag gtctcgcgag acgagctgca cgaagctctt catcagcagc ccggatccaa
     2341 tcagcaggac gaatgtcacc gcgatctgcg ttgcgacgag ggcgcctcga aagcgcggag
     2401 gcagccccga gacgctggca atcggcgact ccttcagtgt cgccgtgacg cccgtccgcg
     2461 ttcccatcag cgccggcgcg aggccgaaca gaatgcccgc cgcgatcgag atcagcgccg
     2521 tcacgatcag gcttcgcaga ttcagagtga cggcgacgat ttcgacgcct ccgggcgggg
     2581 gcgtcatccc caccagcgac cggatgcctc cccaggccac cagcagcccg agtgccccgc
     2641 ccgccaccga caggaggatg ctttcggtga gaagctgccg cacgattcgg ctgcgtccgg
     2701 cgccgagggc ggtgcgcatc gccatctcgg gcatgcgcac gaggccgcgc gacagcagca
     2761 gcccggccag gttcgcgcac gcgacgagca ggaccagcgc cacggcagac ccgagcgtca
     2821 cgaggggcgc caggctccag ccgagcatcg cctcccgcaa cggcttcacc cggacgcccc
     2881 acccctcgtg ccagtccgga tcgtccctgg cgagccgtgc agcaatgatg tccaggtcgg
     2941 attgggcctg ggagatcgtg acgccgtctt tcaggcgggc ggtgacgaca taaaagcgct
     3001 gcgggctctg ccgctgtgat cgatccaggc gaagggggat ccagtagtcg gcgccctgat
     3061 tggggaaccg gaatccctcg ggcatcacac cgacgatcgt ccagttgacc cggtcgagtc
     3121 gtacctgctc tccgaggatg ccggctcgcc cgccgaagcg gcgctgccag agccgatggc
     3181 tgatgacgat gagacgctcg ggagcttcga tatgcgcttc agccgcagtg aagacacggc
     3241 cgatgatcgg ttgcaccccc aggacggcaa gcgcttcggc cgtggccagt tgcccgttga
     3301 cccgctcggc aggcgtgccg gcggcgtcgg ctccaaagtc cgcctgatgg ccgagcgaga
     3361 cgcccatcag atcgaacgag tggctcgcgt cccgccattc gtagtaatcc gagatcggcg
     3421 catgcgtttc ctgacggggg tcgtcgagag ggaacgtgcg cgccacgacc acgcgctcgt
     3481 cgtccggaat gggcaggttg tcgcgaaaga ccgcgtccat cacgctgatg atcgcggtgt
     3541 tggcgccgat gcccattgcg agcgagagca cggccacgag cgtgaacccc gggttcttcc
     3601 tgagcatgcg cacgccgtag accacgtcgc ggcgcacatc gtggatccac gtgaccgatc
     3661 ggtggtcgcg gcactgctcc tcgagagacg gaatgtttcc aatggcccgg cgcgcggcgt
     3721 ggcgcgcgtc ctcgagcggc atgccccgct cggcgaactc ctggaccagt tgctcgagat
     3781 ggaaggcaag ctcccggttc agctcctcgt caacggaggt gcggcggaac agcgcgcgaa
     3841 ggcgctggcg caggacgcga agcagacgcg atatcgacat gatctgagcc tgagccgtgc
     3901 agtctgaggc ctgggccctg ggccctgagg ccttctttgt tcaggcccgc tcgagcagca
     3961 gtccgacggc ggaggacagg cgcttccagg attcgagctc gcgctcgagc tgccgcttgc
     4021 cctggcgcgt cagggagtag aacttcccgc tgcggccctg ctcggtgtcc ctccactcgg
     4081 cggatatcca cccctgttgt tcgagccggt gcagcgcggg gtacagcgat ccctgctgga
     4141 ccgccagcac ctcgttggac agaagctgga tgcgtttgga gatcgcccag ccgtggagcg
     4201 attcccggac cagcgtcttg agaatgagca ggtcgagcgt gccctggagc agatcggatg
     4261 gtttcggcat gaagccctcg cttgacaagc atacctcgat tatcgacaat tcgataccta
     4321 gatgatcgac actagtagca cccccgtcgc ccccctcctg aggagaggcg tcctgctcct
     4381 cggggccgtc gtatgcgccg gcgccgtgct gtcggccggg gatggccgcg cgcgtctgca
     4441 gatgaacgcc gtgacgggcg ggacgataac gccctttgcc ccggcaggga aggccggcgt
     4501 ggtcttcttc gtggccaccg actgtcccgt gtcgaactcg tacgcgccgg agatccagcg
     4561 cctctgccgc gagtacgggc cccggggcgt cggctgctcg ctgatctacg aggacgtgga
     4621 cgtccatccc accagcgcgc atc
//
LOCUS       DBNGSH010000545         1662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1920760, whole genome shotgun sequence.
ACCESSION   DBNGSH010000545 DBNGSH010000000
VERSION     DBNGSH010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1920760"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="AC16CY_12485"
     CDS             <1..225
                     /locus_tag="AC16CY_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3354656.1"
                     /translation="LTYPMSTGRNFDEILRLVDSCRLTAARKVATPVNWKQGEDVIIL
                     PAVSDEDAKKTYPNGWKSPLPYIRIVPQPR"
     gene            complement(319..1098)
                     /locus_tag="AC16CY_12490"
     CDS             complement(319..1098)
                     /locus_tag="AC16CY_12490"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3354657.1"
                     /translation="MRREVGLIVSGVWLAAIPAAAQVSLGSGQVPGITITRDSPSPSP
                     PPPPSPQHPIAGQPSRPIAGPMVPTGGDQFQADPLTYAPRYQTRGWRNDRRRGIPGYG
                     YGFGYSSYGPGYVPYVGVETPYSQPEAEGRLYLRVIPGTAQVFVDGLYVGVADDFSGG
                     GLSLEAGPRRVELRADGYETVTFDVRIVPGEPVNYRKNLARMEARPEAPRVAAIPKTF
                     YVIPRCYAGDSPPQAEDLPRGCRVANLRTIPPIVSSASPQR"
     gene            1155..>1662
                     /locus_tag="AC16CY_12495"
     CDS             1155..>1662
                     /locus_tag="AC16CY_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3354658.1"
                     /translation="MTLRVSLAVIGGFVVGVAAQEPSPKPAAPAAPAFEVASVKPNRT
                     ARNNAMSMGSPPGRFTATNIPLRWIIQNAYPDVVRMSSQLVGGPGWIDSDRFDITAKL
                     PDGALPEQTGLMLQSLLAERFKLVVHKETRETQIYALVLARDDGRLGPRLKRTTVDCA
                     AIKAERRAA"
BASE COUNT          294 a          556 c          543 g          269 t
ORIGIN      
        1 ctgacgtacc ccatgagcac cggccgcaac ttcgacgaga ttctccggct ggtggactcg
       61 tgccgcctga cggccgcgcg gaaagtcgcg acgccggtca attggaagca gggcgaggac
      121 gtgatcatcc tgcccgccgt gtcggatgag gacgcgaaga agacgtaccc gaatggctgg
      181 aagtcgcccc tgccgtatat caggatcgtg ccgcagccgc gctgacgcgc ggctcaactc
      241 ccgacctcca actcccaacg cccaaaacgg agggcgagtg agctgggagc tgggagttgc
      301 gaattgggag ttgaagcgtc agcgttgtgg cgatgcgctg ctgacgatcg gtgggatcgt
      361 gcgcaagttg gcgacgcgac agccgcgggg cagatcctcg gcctgcggcg gcgaatcgcc
      421 cgcgtagcat cgcggaatga cgtagaacgt tttcgggatg gcggcgaccc ggggtgcttc
      481 gggccgcgcc tccatcctgg ccagattctt ccggtagttc accggctcgc ccggaacgat
      541 tcgcacgtcg aaggtcaccg tctcgtatcc gtccgcgcgc agctccaccc gacgtggacc
      601 cgcctccagg ctcagtccgc cgccgctgaa gtcgtccgca acgccgacgt agagcccgtc
      661 aacgaacacc tgcgccgttc cagggatcac gcgtagatag agacgccctt cagcttccgg
      721 ctgagagtac ggcgtttcca caccaacgta cgggacgtac ccgggtccgt atgacgagta
      781 cccgaagcca tacccgtatc caggtatgcc gcgccggcgg tcgttcctcc accccctcgt
      841 ctgatagcga ggcgcgtagg tcaacggatc ggcctggaac tgatcgccgc ctgtcgggac
      901 catcggtcct gcgatcggcc gcgacggctg gcctgcgatc gggtgctgcg gcgacggcgg
      961 cggaggaggc gacggggagg gcgaatcccg cgtgatcgta atgccgggaa cctggccgct
     1021 gccgagcgat acctgagccg ccgccgggat cgcggccagc cagacacccg acacgatgag
     1081 accgacctct cgtctcatgc cacctttata cacccacggc cattccgttg tataaccagc
     1141 cagggcagtc gcccatgacg ctccgagtct cgctcgcggt gatcggcgga tttgtcgtcg
     1201 gcgtcgctgc gcaggagccg tctcctaaac cggccgcccc tgctgctccc gctttcgaag
     1261 tggcgtcggt caagccgaac aggacggcgc gcaacaacgc gatgtcgatg ggttcgccgc
     1321 ccgggcgctt cacagccacg aacattccgc tgcggtggat catccagaac gcgtaccccg
     1381 acgtcgtgcg gatgtcttcc cagctcgtgg gcgggcctgg gtggatagac tcggatcgct
     1441 tcgacatcac ggcaaagctt cccgacggcg cgctgcccga gcagaccgga ctgatgctgc
     1501 agtcgctgct cgccgagcga ttcaagctgg tcgtccacaa ggaaactcgc gagacgcaga
     1561 tctacgcgct cgtccttgcc cgcgacgacg gcagacttgg ccccaggttg aaacgcacga
     1621 cggtggactg cgcggcaatc aaggcggagc ggcgggcggc ac
//
LOCUS       DBNGSH010000546         3687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1920907, whole genome shotgun sequence.
ACCESSION   DBNGSH010000546 DBNGSH010000000
VERSION     DBNGSH010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1920907"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="AC16CY_12500"
     CDS             complement(<1..643)
                     /locus_tag="AC16CY_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3354659.1"
                     /translation="MASKRSRKSTPSLFDLADADAAGSPPPPGGAQGVALHEAAQSRY
                     LNYALSVITARALPDVRDGLKPVQRRILFTMWQQNLTADAKHRKCAKVVGDTMGSYHP
                     HGEVALYETLVRMAQSFSLRYPLVDGSGNFGSLDGDSAAAMRYTECRLARISDEMLTE
                     IEQRTVPFRPNYDGTKSEPVVLPARVPNLLVNGATGIAVGMATNVPPHNLGEVC"
     gene            858..3449
                     /locus_tag="AC16CY_12505"
     CDS             858..3449
                     /locus_tag="AC16CY_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3354660.1"
                     /translation="MIDQTVSHYRIVEKLGSGGMGVVFKAEDLRLGRLVALKFLSETF
                     ARDQRALERLKREARVVAALEHENICAIYDIGEHEGTCFIVMQLLDGMTLADRLGGGP
                     LEVDEVLTFGIEIASALDKAHRTGIIHRDLKPGNVFITSDGHVKLLDFGLAKQHLESA
                     DQSVLPTIAPPLSTPGMAVGTAAYMSPEQARGEDVDERTDLFSFGTVLYEMVTGKPAF
                     GGSTAAVVFDSVLNKTPESAMRINPAVPSLLGDVIGKALDKDRETRYQSAAEMRVDLK
                     RLQRASMSSQALPPAVVPRRAGRLARWAALAGAAAVIAVVGFAIRERGTPDRERPDYR
                     LRLFLSTSEVADRASLASDARMIAYTSVENGQSDIFLSRAAGGGRVRVTNDAATESGP
                     QLSPDGETILFTRLQPGREDEPEICTTPAFGGAIVPIVRGGRAPAWSPDGMRIAFVRQ
                     QPGMAPAVVVSSANGGSVQVVLEADATYIGFGQLAWSPDGSQLAVERSMGGVSGEIWI
                     VPASGGQARRVWQDSPEVFSHSPVFTPDGSGIVHSSNRGGATNLWLMNTRGEAPVRLT
                     TGPGPDEFPSMSRDGTIAFSNSRYRYLLLLQDTELGKARELEGHSSYLWAPASSPTQP
                     EAAVSRLEADGSWHIWIVPLTGGMSRRLTSGTLPEIYPRFTPDGNTVIYSTWSSQPDR
                     VWRVSRAGGPPAPVTPAREEDDAYADVSPDGKWVAFARTETNVTRVHVAPLDGGPARR
                     LTDTPSTTPRWSPDGRWIAYSPERSAGAGIFVVAADGTGARRLAPTGGWPVWWPDGTR
                     VAYWTVGPSGSQEIYVVPIAGGAPARLTQATFTGTNQPFDITADGQLLRTDGVHMNTE
                     VWLMEPQ"
     gene            complement(3459..>3687)
                     /locus_tag="AC16CY_12510"
     CDS             complement(3459..>3687)
                     /locus_tag="AC16CY_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015032184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3354661.1"
                     /translation="ARGRSGLNVWIPVREETTTVQALAGRGWAVAAGERFRLQSPPAI
                     RVTTAALTPEDARRFAADLAASARPAASVHA"
BASE COUNT          606 a         1213 c         1280 g          588 t
ORIGIN      
        1 tacacacctc gccgaggttg tgcggcggca cgttggtggc catgccgacc gcgatgccgg
       61 tggcgccgtt gacgagcagg ttcggcaccc gcgccggcag gacgaccggc tcggacttcg
      121 tgccgtcgta gttgggccgg aagggaacgg tgcgctgttc gatctcggtc agcatctcgt
      181 ccgagatccg cgcgagccgg cactcggtgt agcgcatggc cgccgcgctg tcgccgtcga
      241 gcgacccgaa gttgcccgag ccgtcaacga gggggtaccg caacgaaaag gactgggcca
      301 tgcggaccag cgtctcgtag agcgcgacct ctccgtgcgg gtggtagctc cccatcgtgt
      361 cgccgacgac cttcgcgcac ttgcggtgct tggcgtcggc cgtgaggttc tgctgccaca
      421 tggtgaagag gatgcggcgc tgaacgggct tgaggccgtc gcggacgtcg gggagcgcgc
      481 gcgcggtgat gaccgagagc gcgtaattca ggtaccggct ctgggctgcc tcgtgcagcg
      541 cgacgccctg ggcgccgccg ggcggcggcg ggctgcccgc cgcgtcggcg tcggcaagat
      601 cgaagagcga aggtgtcgat ttcctggatc gcttggaggc cactagttgc tcgagaactc
      661 gccggggtcg gtcaaaggta gcacggctgt cgagaggtgt tctctgctac tcgcttgtgt
      721 tgggggactg cggccaccgc cgcgctcact aaacggccga tcgtgctcat gacaagtctt
      781 ggcgaaatca ccgacacaag cgagcttcgg aggtagactt cgggggcatt gcgcacgaga
      841 cagcgtgagg ccctccggtg atcgaccaga cggtctcgca ctaccgcatc gtcgaaaaac
      901 tcggcagcgg cggcatgggc gtcgtgttca aagccgagga cctgcggctc ggacggctcg
      961 tggcgctgaa gttcctgtct gagacgttcg cccgggacca gcgtgccctc gagcggctga
     1021 agcgggaagc ccgggtggtc gccgccctcg agcacgagaa catctgcgcg atctacgaca
     1081 tcggagagca cgagggcacc tgcttcatcg tgatgcagct gctcgacggg atgactctcg
     1141 cagaccggct cggtggcgga ccactcgagg tcgacgaagt cctgacgttc ggaatcgaga
     1201 tcgccagcgc gctcgacaag gcccaccgca ccggcatcat tcatcgcgac ctcaagccgg
     1261 gaaacgtgtt catcacgtcg gacgggcacg tcaaactgct cgacttcgga ctggccaagc
     1321 agcatctgga aagcgccgac cagtcagtgc tgcccaccat tgctccgccg ctcagtactc
     1381 ccggcatggc cgttggcacg gctgcctaca tgtcgcccga gcaggcacgc ggcgaggatg
     1441 tggacgagcg caccgacctg ttctcgttcg gcactgtcct ctacgagatg gtgacgggca
     1501 agccggcgtt tggcggctcg acagccgccg tggtgttcga ttcggtcttg aacaagacgc
     1561 ccgaatcggc catgcggatc aatcccgccg ttccatcgct gctcggtgac gtcattggga
     1621 aggcgctcga caaggaccga gagactcgat accagagcgc ggcggagatg cgggtcgatc
     1681 tgaaacgcct gcagcgcgcg tcgatgtcga gtcaggcgct tccgccggcg gtggtccctc
     1741 gacgcgccgg ccggctggcg cgatgggcgg cgctggcggg ggccgccgcg gtcatcgcgg
     1801 tcgtcgggtt cgccattcgg gaacgcggca cccccgatcg tgaaaggccc gactaccgcc
     1861 tgcgactctt tctgtccacg agtgaggtcg ccgatcgcgc ctcgcttgcg tccgacgcga
     1921 gaatgatcgc gtacacctcc gtcgagaacg ggcaatcgga catcttcctc agccgcgccg
     1981 ccggaggcgg ccgtgttcgc gtcaccaatg acgcggccac cgagagcggg ccgcagctgt
     2041 ctcccgacgg tgaaacgatt ctcttcacca ggctgcagcc gggcagagag gacgagccgg
     2101 agatttgcac gactcctgcg ttcggcggcg ccatcgtgcc aatcgtgcgc ggtggcaggg
     2161 ccccggcgtg gtctcctgac ggcatgcgca tcgcgttcgt acgtcagcag cccgggatgg
     2221 cgccagccgt cgtcgtctcg agcgccaacg gaggcagcgt gcaggtcgtc ctggaggccg
     2281 atgcgaccta catcgggttc ggccagctgg cctggtcgcc cgacggttcg cagctcgccg
     2341 tcgaacgcag catggggggc gtgagcggag agatctggat cgttccggcc tcgggcgggc
     2401 aggccaggcg cgtatggcag gactcacccg aggtcttcag ccacagccct gtcttcacgc
     2461 cggacggatc cggcatcgtg cactcctcga accgtggagg ggccacgaac ttgtggctga
     2521 tgaacacgcg cggcgaggca ccggttcggc tgacgacagg acccggcccc gacgagttcc
     2581 ccagcatgtc gcgcgacgga acgatcgcct tttcgaattc gcggtaccgg tatctcctgc
     2641 tgctccagga cacagaactc ggaaaggcgc gggagctcga gggacattcg tcgtacctct
     2701 gggcgccggc ctcctcgccc actcagccgg aagcggcggt gagccgactc gaggcggacg
     2761 gatcgtggca catctggatc gtgccgctga ccgggggcat gtcgcgacgc ctgacatccg
     2821 ggactctccc ggaaatctac ccgcggttca cacccgacgg aaacacggtg atctactcga
     2881 cgtggagctc ccagcccgat cgcgtctgga gagtgtcgcg cgcaggtggt cctccagcgc
     2941 cggtcacgcc ggctcgcgag gaagacgacg cctatgccga tgtgtcgcct gacggcaaat
     3001 gggtggcatt cgccaggacc gagacgaacg tgacccgcgt gcatgtggcg ccactggatg
     3061 gagggccggc acgccggctc acggatactc catccaccac gccccgctgg tcgccggacg
     3121 gacgatggat cgcgtacagc ccggagcgat ccgcaggcgc cgggatcttc gtcgtggccg
     3181 ccgacggcac gggcgcgcgc cggctggcac cgacgggcgg gtggcccgtc tggtggccgg
     3241 atggaacgcg ggtcgcgtac tggacggtgg gaccgtcggg cagccaggag atctacgtcg
     3301 ttccgatcgc cggtggcgcc ccggccaggc tcactcaggc gaccttcacc ggcacgaatc
     3361 agccgttcga cattacggcc gatggccagc tgctcagaac cgacggtgtt cacatgaaca
     3421 cggaagtgtg gttgatggag ccgcagtagc ccggcgcctc aggcatgcac cgacgcggcc
     3481 gggcgagccg acgccgcaag atcggccgcg aagcgtcgag catcctccgg cgtgagcgcc
     3541 gccgtcgtca ccctgatggc cggcggactc tgaagacgga accgctcgcc cgctgccacg
     3601 gcccatccgc ggccggccag cgcctgaacg gtcgtcgtct cttcacggac tggaatccac
     3661 acgttcaggc cggatcgtcc gcgcgcg
//
LOCUS       DBNGSH010000547        13738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4297983, whole genome shotgun sequence.
ACCESSION   DBNGSH010000547 DBNGSH010000000
VERSION     DBNGSH010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 13738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13738
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4297983"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="AC16CY_12515"
     CDS             complement(<1..1219)
                     /locus_tag="AC16CY_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3354662.1"
                     /translation="MPQVDHADVLTAVARLVAEAPSLRDVVSRLAGALRAAIPFERLH
                     LLRLDRADSFVLYVARDTGEIDVAKHLVGDPATQVDQVDAAVQSRIVCTIRQGARVHG
                     ALWLTSSRESAFCDAHQALMDGVVDLLGLALEHNAIVERETLRRERIDSLRGLLHTMA
                     GALDIRHVFDKVSEVVRGGLPHDMLVITSWGEDGASFRFYAMAGATVEDPEFWEPQAI
                     TGDDLAQLQRDAYVVHDADEEIPADSLRGKVFRRFGLRSALRVLMPLGTEVFGSLFFL
                     SRQTDRYSEDDVDFARRVADHLALALSHERLAEAARTQAKSLETAARLEAQVATLTRE
                     LESRTGQRRVVGQSRQWREVLAHAARVAHTETTVLLTGESGTGKEVVARFIHHASRRG
                     QGPFVAINCAALPD"
     gene            1252..2181
                     /locus_tag="AC16CY_12520"
     CDS             1252..2181
                     /locus_tag="AC16CY_12520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354663.1"
                     /translation="MASSDAANALGPGLSWIDLKFQGRTRVIATALVIGRGGVALIDP
                     GPTSCLETLELGLQAHGIRWPDVRQILLTHVHLDHAGATGTIIRDHPHLKVLVHERGA
                     KHLADPTRLIDSATRIYRDQMDRLWGEIAPVPAQNLEPLAGGERVEAGGRHLEVAYTP
                     GHASHHVAFFDESSGVAFVGDTAGVCIDEGYVLPPTPPPDIDVEAWSASVDRILSWSP
                     GTLLLTHFGPVGRVRPHMAALLENLEAAAAMVRALLNGPGTDEDRTAAFAEEMPRRLR
                     QHMTEEQIGAYLIASGFEHAFAGLARYWRKKSP"
     gene            complement(2209..2502)
                     /locus_tag="AC16CY_12525"
     CDS             complement(2209..2502)
                     /locus_tag="AC16CY_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354664.1"
                     /translation="MSVVTRDVSEVDAFVECQVPASIPLYRLVHLQLERPTQDADVPT
                     ALRDGKVLSAVYRVGPYKTSTGTPTGYALRLLVDKASAAAASRPSGARLAVAN"
     gene            complement(2817..5762)
                     /locus_tag="AC16CY_12530"
     CDS             complement(2817..5762)
                     /locus_tag="AC16CY_12530"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3354665.1"
                     /translation="MPAPPLSSTLARIASALERGRGSEVLPLLQPLLRKGSLSRDDEL
                     AVRAALAEAWLLQDDLTQAAAVLGRPPDSLREPVSDARLSTLWRLHGRVAFARGEQSR
                     AIALHTRALRHADLAHESRAIGLANYELALCYKQVGDSGIVREHLTQAASALHAVGDR
                     RHLALIHSLSAVLLAQSGRCDEAAAALRQGERLAMAVPADDVLAGIVHNQANVALMQH
                     KHEQALALAERSVALHQSRGSGHGLAVALATLGQIFVQLGDLERAEQVLTRTLEVRST
                     IQFHETTGAVFDTLTQIHLMRGSYDRAEQYLRLAAEAYGGYGTQTMRWYEWSLKVLGV
                     KLATRRQAYDEAVTMADALASAPGVPQSDIIQAELAACDALITAGRIQEAERRLEACE
                     GRLDPRGAPANWGEFLRIRGLIREQSGRPDAARHDFAQSSNVFDLLGERYQAALSQLA
                     LGRLTARLGLSAVAERHLDHAAAEFKTLGAQRDLDEAVAIRSRLAAAPASSPVAQTTD
                     AHEGIVRRLVDAAIFKDLLARETASALLEAVDASAAVVFVESSGGDVRVVAAAGCDAV
                     AAAGLAHAARQGQSAHAGGPLLVDSLGKETDGARRCAIALTRQLDGVSDARLRMFTAI
                     ARQGFELCGARERPVPSVDQLIERPIEPLLPGFICAGPAMHRLADQVQRLQGHNLTVL
                     ITGESGTGKDLVARAIHVGSPRSAAMFLPYNCTTTTRDLADSQLFGHRRGSFTGAITD
                     QQGLIRSAAGGTLFLDEIGDLPLDIQPKLLRFLEQGEVMPVGETRPQAVDVRVLAATN
                     ADLEQRVSEGKFREDLYYRLSVIRIQVPPLRDRREEVPHLSTFFLRDACERLGKPDIQ
                     LGAATLDLFARYWWPGNVRQLRNEIQRAVAMSPPGGTIEPDNLSPDLTAPERQQAPGV
                     PALPDRAPAQPGNLAAAVERVERDMIMATLDRTRGNVSETARVLGLTRRGLYLKIRRL
                     GLESVAADI"
     gene            5814..6536
                     /locus_tag="AC16CY_12535"
     CDS             5814..6536
                     /locus_tag="AC16CY_12535"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3354666.1"
                     /translation="MPLAASGVTHQGRRSTNEDTWLVDLDLGLLVVADGMGGHNAGEV
                     ASTLAVEVIREFLKSRSVPPTRAMLEEAVESANERILSDAARYPDYAGMGTTVVAVVV
                     SGDRVFYTSVGDSRLYLWHRGQLTQLTRDDSWLAEALGQDEAMRSEHVGAHPMRHVLT
                     RVVGLRPELQAKAAEYTFDADDCLLLCSDGIHGVVPHEMLADILASEHNVADIAQGVV
                     RSAIARGATDNATVVVARLATG"
     gene            complement(6544..8226)
                     /locus_tag="AC16CY_12540"
     CDS             complement(6544..8226)
                     /locus_tag="AC16CY_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR3354667.1"
                     /translation="MTAPLAFPVTARSLTESFEAACARLDTFGFADALSFRRLDVWSA
                     DAETQRLIGNRLGWMQAVAFATSQVPRLRAFADSVKRDGFTDVVLLGMGGSSLAPAVL
                     ADVFGPAPGSPRFRVLDSVDPDAVRPAMVRAATTLFVLASKSGSTIEPNAMAEEARRR
                     VIGHGHESWGTRFVAITDEDTALHRRAAAETFRDVFVNPADIGGRYSALSLFGMVPAA
                     LMGIDLDALLAGARVMEAACRTPRAKDNPGLALGAFIGAGVLAGRDKLTLQLPPELQS
                     FGLWVEQLVAESTGKQGTGVVPILGESRESKLGPDRLVVTISLGTPPSGDSGLDAGVP
                     HMSLGMPNLEALGGEFFRWEVATATAGLLLGVNPFDEPNVQQAKSATGALLDTYRQRR
                     ELPSPEPHAVLGGIPVTLSGPARARLSGHPAQSFLRLARGTDYVAVLAYLPPEDPAWD
                     QALRRFRAGVTARTGCAATLGYGPRYLHSTGQLHKGGPPSGVFLIVTAETHEDLAVPN
                     APFSFGVLEMAQAHGDFTSLERTGRRVMLARLPRRDVGMLQQFCDELLAGEW"
     gene            8260..8970
                     /locus_tag="AC16CY_12545"
     CDS             8260..8970
                     /locus_tag="AC16CY_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR3354668.1"
                     /translation="MTRFLTRLVLLLVVASCACAQPGPAAERTPPPSGPVAVAPAAST
                     APRIVFLGDSLTAGLGLPMEASVPALIERRLQDEGYEYRVVNAGVSGDTSAGGLTRLE
                     WSLEGDVKVLVIELGANDGLRGLPVTNLKTNLAEVIVRARSRGIRVVLTGMEAPPNFG
                     AVYTSEFRDVYRQLAREHDVIFVPFFLEGVAGIPALNQSDGMHPNAQGARVIEQTIWR
                     ALEPLLTKATHGRGNGTR"
     gene            8967..9668
                     /locus_tag="AC16CY_12550"
     CDS             8967..9668
                     /locus_tag="AC16CY_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3354669.1"
                     /translation="MIELRGVSKTVRSGDHPLTILHPLDYTIPSGQFIAIVGPSGSGK
                     STLLGLLAGLDAPSTGQIVIDGTDITGLSEDALARLRGDKIGFVFQFFHLVPSLTALE
                     NVLIPMEIARRKDAMARAKQLVDEVGLAERAHHYPSQLSGGEQQRIAIARALANDPPI
                     VLADEPTGNLDSTTGRHIMDLLLNVRRSRRSTLVLVTHDAELASLADTRLVLRDGRQV
                     PQASAVTAAGAEVAR"
     gene            9665..12220
                     /locus_tag="AC16CY_12555"
     CDS             9665..12220
                     /locus_tag="AC16CY_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354670.1"
                     /translation="MTFIVRMAMREIRASWRRLLFFFVCIAVGVAAIVAIRSLIQSVR
                     EGLTQEARAMTGADVVVRSDRPLGDAVRAAVARERASGRVGVVYEAVEMATMVRPSGA
                     PSARMVELRAVQDAFPLYGTMTLSEGTYSHSLLRGHGALVRPELLTQLGLRVGDDLLI
                     GTERFQIRGVIESEPGRNLGAFSLGSRVFIDLADLASTKLLSFGSRASYELLLQVPGP
                     PPVPGRKDVSTVMASELSDAFVNDFVRVRSYRQNEGRMNQNLTRAENYLSLVGLVVLI
                     LGGIGVSSVTRVFVQQKVRAIAILKCVGSTTRQVLAVYLTQVVLLGTAGSMLGVGIAS
                     AALAALPVFVGDLAAIFKVDYGLTAGAVTQGLAVGLLVSLLFSIVPLLEVRNVKPSLL
                     LRQDVPPSPGVDWLKWIVATAVASSLVAVASWQAGSLRVGLVLSGGFVAVAFVLHLAG
                     AALIRAVQPLRHSRSFALRQAVLHVARPGNQTRIILLAVGLGAFFILGARALQVNLLK
                     DFSLQVGEGAPDMFLMDIQATQRQSLLALIDRENGPDGPPKLIPVLRARIVGVRGRDV
                     NLESYEDVRGRGGLSREFTVTYRSTLEPNEKLVDGTWWDETPAPDAAEVSIEEGLRAR
                     YQIQLGDEMRFDVLGRIVTARVTSFRAVDFRDFRAGGFMIVFRPGPFDGAPHSFIAAV
                     HGGGDRPARARLQGLLVASHPNVSVIDLREALDSIKSIVDNVTLAVTVVGALVLLSGS
                     LILIGAVSMTKFRRVYEAAILKTLGASSRLIAAMLLLEYGVLGAIAGTVGALGAIALS
                     WAVARYALDMSWEPTPILTIAGIVVTAIAVAAIGVLASLDVLRHKPLSTLRAE"
     gene            12276..12611
                     /locus_tag="AC16CY_12560"
     CDS             12276..12611
                     /locus_tag="AC16CY_12560"
                     /inference="COORDINATES: protein motif:HMM:NF044994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3354671.1"
                     /translation="MDVDHYRDALLRKRGELLGGSASKPLQWTMENNSGRQGDMADQA
                     AGNNEVHIQLKLKQTDAKILQAIEGALRRIEEGTYGVCRDCGEPIAEARLNAIPWTRA
                     CISCKEKQG"
     gene            12614..13123
                     /locus_tag="AC16CY_12565"
     CDS             12614..13123
                     /locus_tag="AC16CY_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354672.1"
                     /translation="MSPDELRSLLTSFTADRLALLSRHEAGAKVVSHYDFNNAYQYVI
                     AREETQLSWLQSALEELGASALPPAAAVIPVPPGPKPARRVSASAFRGILDDDVRHLA
                     DFVKRWRAPVAAMTQARHRTMLQVILGESVEHQRLFEQAAAGLEDVIGRRTTGAERVG
                     AVLPTRWME"
     gene            13162..13632
                     /gene="folK"
                     /locus_tag="AC16CY_12570"
     CDS             13162..13632
                     /gene="folK"
                     /locus_tag="AC16CY_12570"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3354673.1"
                     /translation="MTPDPLFPVAIALGSNLGDRHTSLRAAIDQLRPLLQSMRASSFH
                     ETAPVGVGDQPMFLNAAVVGETKLPAHDVLRALLDIEARHGRERPYPGAPRTLDLDLI
                     LYGGEVIDSPELVVPHPRFRQRRFVLEPLSEIAADWRDPITGRTVSELLLTLSA"
BASE COUNT         2261 a         4572 c         4639 g         2266 t
ORIGIN      
        1 gatcgggcaa cgccgcgcag ttgatcgcga caaacggtcc ctgaccgcgc cgcgacgcgt
       61 ggtggatgaa ccgggcgacg acttccttgc cggtgccgga ttcgccggtc agcagaaccg
      121 tggtttcggt gtgcgctacc cgggcagcgt gcgcaagcac ctccctccat tgcctggact
      181 gaccaaccac acgccgttgt cctgtgcggg attcgagctc gcgcgtcagc gtcgcgacct
      241 gcgcctcgag ccgagcggcg gtctccaggg acttcgcctg agtgcgcgcc gcctcggcca
      301 ggcgctcgtg cgacagcgcc agggccagat gatccgccac gcgccgcgcg aaatcgacgt
      361 cgtcctcgct gtagcgatcc gtctggcgcg agagaaagaa gagcgaccca aagacctcgg
      421 tgccgagcgg catcagcacg cgaagcgcgg atcgcaaacc aaagcgtcgg aagacttttc
      481 cgcgaaggct gtcggccggg atctcctcgt cggcgtcgtg gacgacgtag gcgtcgcgct
      541 gaagctgcgc gaggtcgtcg cccgtgatcg cctgaggctc ccagaactcg gggtcctcca
      601 cggttgcgcc cgccatcgcg tagaagcgga acgacgcgcc gtcctctccc caggacgtga
      661 tgacgagcat gtcgtgaggc agcccgccgc gaacgacctc ggacaccttg tcgaacacgt
      721 gacggatgtc gagggcaccc gccatcgtgt gcagcagccc gcgaagactg tcgatacgtt
      781 cccggcgcag cgtctcgcgt tcgacgatcg cattgtgttc gagagcgagg ccgagcaggt
      841 ccacgacgcc gtccatcagc gcctggtgag cgtcgcagaa ggcgctctct cgcgacgatg
      901 tgagccagag cgcgccgtgc acgcgggcgc cctggcggat cgtgcagacg atgcgcgact
      961 gaaccgccgc gtccacctga tcgacctgcg tcgccgggtc cccgacgagg tgcttggcca
     1021 cgtcgatctc gccggtgtcg cgcgcgacgt agagcacgaa ggagtcggca cggtcgagtc
     1081 gcagcagatg caggcgttcg aagggaatcg cggcgcggag ggcgccggcc aggcgggaca
     1141 ccacgtcgcg aaggctcggc gcctccgcga ccagccgcgc aaccgccgtc agcacgtcgg
     1201 catgatcgac ctgaggcatg gagtgggccc accagtataa tcccgcgcac catggccagt
     1261 tccgatgccg ccaatgcgct cggccccggg ctctcgtgga tcgatctgaa gtttcaggga
     1321 cgaacccggg tcattgccac cgcgctcgtc atcggcagag gaggcgtggc gctgatcgat
     1381 cccggaccga ccagctgtct cgagacgctc gagctcgggt tgcaggccca cggcatacgg
     1441 tggcccgacg tgcgccagat cctgctgacg cacgtgcacc tcgaccatgc cggtgccacg
     1501 ggcacgatca ttcgcgatca cccgcacctc aaagtgctcg tgcacgagcg aggggcgaaa
     1561 cacctggcgg accccacgcg gctgatcgac agcgccacga gaatctatcg cgatcagatg
     1621 gaccgtctgt ggggtgagat cgctccggtg ccggcacaga atctcgagcc gctggcagga
     1681 ggcgaacgtg tcgaagcggg cgggaggcac ctggaggtcg cctacacacc cggacacgcg
     1741 tcacaccacg tcgccttctt cgacgagtcc agcggcgtcg cgttcgtcgg agacacggcg
     1801 ggcgtgtgca tcgacgaggg ctacgtgctc ccgcctacgc cgccgccaga catcgacgtg
     1861 gaggcctggt cggcgagcgt cgatcgaatc ctgtcctggt cgccgggaac gcttctgctg
     1921 actcacttcg gacccgtggg ccgcgtgcgg ccgcacatgg cggccctcct cgagaatctc
     1981 gaggcggcag ccgcgatggt tcgagcgctg ttgaatgggc ctggaaccga tgaagatcgc
     2041 actgcggcgt tcgccgagga gatgccccgc cgcctgcggc agcacatgac cgaagagcag
     2101 atcggcgcct acctgatcgc gtccggattc gaacacgcgt tcgccgggct ggcgaggtat
     2161 tggaggaaga agagtccgta gtcccggtcg gtcagccgtc gtgaatgttc agttggcgac
     2221 agccagacgt gcccctgatg gcctggacgc ggcggcagcc gacgccttgt ccacaaggag
     2281 gcgaagggca tagcccgttg gcgtgcccgt tgacgtcttg tatgggccga cccggtagac
     2341 ggccgatagc actttgccgt cgcgcagggc cgtcggcacg tcagcgtcct gggtcggccg
     2401 ctcgagctgc agatgaacca gccgatacag cggaatcgac gcggggacct ggcactccac
     2461 gaacgcatcg acctcgctca cgtcgcgagt gaccacggac acgaatcgga gagtgccact
     2521 ggcgtcgcgc catgtcagcc gagcaggaag gctgagcttg gtgcggtttc caattcgcga
     2581 ttgctcggtt gtggtctgtt gaatcgctag cgatcgaatc aaggctgccc cccgtcgaat
     2641 gcgttccttg ccggctctac gattcaatgc gcaggcctga gcaattggtg ttactgccgg
     2701 gaaggcagac gtagctggaa agtattgata acaaaatgga tagtttgagt gagtcctctc
     2761 cagcggagga ccgctgaatg gatactcagt atccataccc cctgtataca tggttactag
     2821 atatccgcgg cgacgctctc gagcccaagg cggcggatct tcaggtacag gccgcgccga
     2881 gtcaggccaa ggacccgggc ggtctcggag acgttccctc tggttcgatc cagagtggcc
     2941 atgatcatgt ccctctcgac ccgctccact gccgctgcca gattccccgg ttgagcaggg
     3001 gctcggtcgg gaagcgcagg cacccctggg gcctgctgac gttcgggtgc tgtcaggtcg
     3061 ggggacaggt tatctggctc gatcgttccg ccgggcgggc tcatcgccac ggcgcgctga
     3121 atctcgtttc tcaactggcg cacgttgcct ggccaccagt acctggcgaa gagatccagc
     3181 gtcgccgcgc ccaactggat gtcgggcttt ccgagccgtt cgcacgcgtc gcgaaggaag
     3241 aacgtactca ggtgcgggac ttcctcgcgc cggtcacgca ggggaggcac ctggattcga
     3301 atcacgctca gccgatagta gaggtcctcg cggaactttc cctccgagac acgctgctcg
     3361 agatcggcgt tggtggcggc gagcacgcgg acgtctaccg cctggggccg cgtctctccg
     3421 accggcatca cttcgccctg ctcgaggaag cgcagcagct tcggctggat gtcgagaggg
     3481 aggtcgccga tctcgtcgag gaacagcgtc ccgcctgcgg ccgatcgaat cagcccctgc
     3541 tggtccgtaa tggctccggt aaagctgccg cgacggtggc cgaagagctg actgtcggcg
     3601 agatcgcgtg tcgtggtggt gcagttgtac ggcaggaaca tcgccgcgct tctcggcgag
     3661 ccgacgtgga tggcgcgagc cacgagatcc ttgccggttc cgctctcccc ggtgatgagc
     3721 acggtgaggt tgtgtccctg caggcgctgg acctggtccg cgaggcgatg catcgccggt
     3781 ccggcacaga tgaatccagg caggagcggc tcgatcgggc gctcgatgag ctgatcgacg
     3841 ctcggcacgg gacgctcgcg ggcgccgcac aactcgaagc cctgtctcgc gatggccgtg
     3901 aacatgcgca ggcgggcgtc ggagacgccg tccagctggc gcgtcagtgc gattgcgcaa
     3961 cgccgcgcgc catcggtctc ctttccgagc gaatccacga gcagcggacc accggcatgc
     4021 gccgactgtc cctggcgagc ggcgtgcgcc agaccggctg ccgccaccgc gtcgcacccg
     4081 gcggcggcga ctactcgcac gtcgcctccg gacgattcga cgaagacgac ggcggcggac
     4141 gcatcgacgg cttcgagcag ggccgacgcc gtttcacgcg cgagcaggtc cttgaagatc
     4201 gccgcgtcaa cgaggcggcg cacgatgccc tcgtgcgcat ccgtagtctg agccacgggt
     4261 gagctggcgg gcgccgcggc cagccggctg cggattgcga cggcttcgtc gaggtcgcgc
     4321 tgcgccccca gcgtcttgaa ctcggcagcg gcgtggtcaa gatgccgttc ggcaaccgcg
     4381 gacaagccca ggcgcgccgt gagccgcccc agcgcgagct ggctcaaggc ggcctgatac
     4441 cgctcgccga gcaggtcgaa gacgttcgag ctctgcgcga aatcgtgacg cgcggcatcg
     4501 ggccggcccg actgctcgcg gatcaagccg cggatgcgca ggaactcccc ccagttggcg
     4561 ggtgcgcctc gcgggtccag ccggccctcg caggcttcga ggcgccgctc ggcttcctgg
     4621 atgcgaccgg cggtaatcag ggcgtcgcag gccgcaagct ccgcctgaat gatgtcgctc
     4681 tgtggaacac ccggtgccga cgccagcgca tcggccatcg tgacggcttc gtcgtaggcc
     4741 tgccgtcgcg tcgccagttt cactccgagt accttgagcg accactcgta ccagcgcatc
     4801 gtctgcgtgc cgtagccgcc gtacgcctcg gcggccaggc gcaggtactg ttccgcgcga
     4861 tcgtaagacc cgcgcatcag gtgaatctgc gtcaacgtgt cgaagactgc tcccgtcgtt
     4921 tcgtggaact ggatcgtgct ccgcacttcg agcgtgcgcg tcagcacctg ctcggcgcgt
     4981 tcgagatccc cgagctgcac gaagatctga ccgagcgtgg cgagcgcgac cgccaatccg
     5041 tgacccgacc cgcgggactg gtgcaaggcg acgcttcttt cggcgagcgc aagcgcctgc
     5101 tcgtgcttgt gctgcatcag cgccacgttg gcctggttat gcacgatccc cgcgaggacg
     5161 tcgtcggccg gaaccgccat cgcgagccgt tctccctgac ggagcgctgc cgccgcctcg
     5221 tcacaccggc ccgactgcgc gagcagcacc gccgacagag aatgaatgag ggccagatga
     5281 cgacggtccc ccacggcgtg tagcgccgac gcggcctggg tcaggtgctc gcggacaatg
     5341 cccgagtcgc caacctgctt gtagcagagg gcgagttcgt aattcgccag cccgatggcc
     5401 cgcgactcgt gagcgagatc ggcgtggcgg agcgcgcgcg tgtgaagcgc gatggccctg
     5461 gattgctcgc cgcgggcgaa tgcgacccgt ccgtgaaggc gccacagcgt cgagagcctg
     5521 gcgtcggaaa ccggttctcg cagtgaatcg ggcggacgac caaggaccgc agcagcctgg
     5581 gtcaggtcgt cctgcagcag ccaagcctcg gccagagccg cccggacggc cagctcgtca
     5641 tcccggctca acgagccctt tcgcagcaag ggctgcagca gcggaaggac ctctgatcca
     5701 cggccgcgct cgagtgcgct cgcaatgcgc gccagggtcg aggacaacgg cggtgcaggc
     5761 acgtaaggag tatacaaagt atacacgcga aggaaagcta taatcggtgg cccatgccgt
     5821 tggcggcgtc tggcgtgacc catcaggggc gccgctccac aaacgaagac acttggcttg
     5881 tcgacctcga cctcggcctc ctcgtggtgg ccgacgggat gggcggtcac aatgcgggcg
     5941 aagtcgcctc gacgttggcg gttgaggtga tacgtgaatt cctgaagagc cgcagcgtcc
     6001 cgccgaccag agcgatgctg gaggaggccg tcgagtccgc gaacgagcgc attctgagcg
     6061 acgcagcgcg gtatcccgac tacgcaggga tgggtacgac ggtcgtcgcc gtggtcgtat
     6121 ccggcgatcg cgtcttctac accagcgttg gcgacagccg cctgtacctg tggcatcgcg
     6181 ggcaactcac gcaattgaca cgggacgact cgtggcttgc cgaggcgctc ggtcaggacg
     6241 aagcgatgcg gtcggagcac gtcggcgccc atcccatgcg ccacgtgctc acccgtgtgg
     6301 tcggcttgag gcccgagctg caggcaaagg cggctgagta caccttcgac gccgacgact
     6361 gtctgctgct gtgcagcgac ggcattcacg gcgtcgtccc gcatgaaatg ctcgccgata
     6421 tcctggccag cgagcacaac gttgctgaca ttgcccaggg cgtggtgcgg tccgcgattg
     6481 ccaggggcgc gaccgacaat gccacggtcg tcgtcgctcg attggctacc ggctagctac
     6541 cggctaccac tcaccggcga gtagctcgtc acagaactgt tgcaacatcc cgacgtcgcg
     6601 gcgggggagc cgcgccagca tcacgcgtcg gccagttcgc tccagcgacg tgaaatcgcc
     6661 gtgcgcttga gccatttcga gcacgccgaa tgagaacggc gcgtttggga cagccaggtc
     6721 ttcgtgggtt tcggccgtga cgatgaggaa cacgccgctc ggcggaccgc ccttgtgcaa
     6781 ctgaccggtg gaatggagat accgcggacc gtatccgagt gtcgcggcac acccggtccg
     6841 ggccgtgacg ccggcgcgga agcgccgcag cgcctggtcc caggccggat cctccggtgg
     6901 cagatacgcc agcacggcga cgtaatcagt tccgcgcgcg agccgcagga acgattgagc
     6961 cggatggcca gagaggcggg cacgcgcggg accgctgagc gtgaccggta tgccgcccag
     7021 aacggcgtgc ggctcgggag agggaagctc ccgccgctga cgataggtgt cgagcagcgc
     7081 tccagtcgca ctcttggcct gctgcacgtt gggttcgtcg aacgggttga cgccgagcag
     7141 caatcccgct gtcgccgtcg ccacctccca tctgaagaac tcgccgccga gtgcttcgag
     7201 attgggcatc ccgaggctca tgtgaggcac gccggcatcc agcccgctat caccagacgg
     7261 gggagtcccg agcgatatcg tgacgaccag acgatcgggt ccgagcttcg actcccggga
     7321 ctcgcccagt atcggtacga cgcccgtgcc ctgcttccct gtgctctccg ccacgagttg
     7381 ctcgacccac agcccgaatg attgcagctc cgggggtagt tgcagcgtca gcttgtcgcg
     7441 gccggcgagg actcccgcgc cgatgaaggc gccgagcgcc aggcccggat tgtctttcgc
     7501 ccgtggagtc cggcacgcag cctccatgac ccgggcaccg gccagcaacg cgtcgaggtc
     7561 gattcccatc agcgccgccg gcaccattcc gaacaatgaa agcgctgaat accgtccccc
     7621 gatgtccgcc gggttcacga acacgtcgcg aaatgtctcc gcggcggcgc gacggtgaag
     7681 cgcggtgtcc tcgtcggtga tggcaacgaa ccgcgttccc cacgattcat gtccgtggcc
     7741 gatcacgcga cgccgggctt cctccgccat cgcattcggc tcgatggtcg atccggactt
     7801 gctggccaga acgaacagcg tcgtcgcggc gcgtaccatc gccgggcgca cggcatcggg
     7861 atcgaccgag tcgagcacgc gaaaccgtgg tgatccaggt gccggtccaa acacgtcggc
     7921 cagaactgcc ggagcaaggc tggagccgcc catgccgagg agcacgacat ccgtgaagcc
     7981 gtcccgcttc accgagtcgg caaacgcacg aagccgcgga acctgtgagg tcgcgaatgc
     8041 aaccgcctgc atccaaccga gcctgttgcc gatgagccgt tgcgtttccg cgtcagcgct
     8101 ccacacgtcg agacgcctga acgacagcgc gtccgcgaat ccaaacgtgt ccagccgcgc
     8161 gcatgccgcc tcgaatgact ccgtcagcga tcgagcggtg acaggaaacg ccaacggggc
     8221 agtcatccga tgaatctata acataatgat ccgagcgaca tgacacggtt cctgacgcgc
     8281 ctcgttctgc ttctcgtggt cgcgtcgtgc gcgtgcgcgc agccggggcc cgcagcggag
     8341 cgcacgccgc cgccctccgg cccggtggca gtggcgccgg ccgcctcgac ggctcctcga
     8401 atcgtgtttc tcggagatag cttgacagcg ggcctgggcc tgccgatgga agcgtcggtg
     8461 ccggcgctga tcgagaggcg cctgcaggac gagggctacg aatacagggt tgtcaacgcg
     8521 ggcgtgtcag gcgacacgtc cgccggcggc ctcactcggc tggagtggtc gctcgaggga
     8581 gacgtgaagg tcctcgtgat cgagctcggc gccaacgacg gtcttcgcgg gttgccggtg
     8641 accaacttga aaacaaacct cgccgaggtc atcgtcaggg cgcgaagccg cggcatccgc
     8701 gtcgtgctga cgggcatgga agcgccgccg aacttcggtg ccgtctatac gtctgagttc
     8761 cgtgacgtct accggcagct ggccagggag cacgacgtga tctttgtccc gttctttctc
     8821 gaaggcgttg ccggcatccc ggccttgaac cagtcagacg gcatgcaccc caacgcccag
     8881 ggcgctcgcg tgatcgaaca gacgatctgg cgcgcgctcg aaccgctgct cacgaaggcg
     8941 acccatggcc gggggaacgg tacgcgatga tcgagctgcg gggggtgtcg aaaacagtcc
     9001 ggagcggcga ccatcccctg acgatcctgc atccgctcga ctacacgatc ccctcgggac
     9061 agttcatcgc catcgtcggt ccctccggga gcggcaagtc cacgctgctt ggcctcctcg
     9121 cgggactcga cgcgccgtcc acgggacaga tcgtcatcga cggcaccgac atcacgggac
     9181 tgagcgagga tgcgctggcg cggctgcgcg gcgacaagat tgggttcgtc ttccagttct
     9241 tccatctcgt gccgtccctg acggcgctcg agaacgtgct gattccgatg gaaatcgccc
     9301 gccggaagga cgcgatggcg agagcgaagc agttggtgga cgaagtcgga ctggccgagc
     9361 gcgcgcatca ctatccctcc caattgtccg gcggcgagca gcagcgaatc gccatcgcac
     9421 gcgcgctggc caacgatccg ccgatcgtcc tggccgatga gccgaccggc aacctcgact
     9481 cgacgaccgg acgccacatc atggacctgc tgttgaacgt ccggcgatcc agaaggtcca
     9541 cgctcgtgct ggtgacgcac gacgccgagc tggcctcgct ggccgacaca cgtctcgtgc
     9601 ttcgtgacgg gcgacaggtg ccgcaggcct ccgccgtgac ggcggccggc gcggaggtgg
     9661 cacgatgacc ttcatcgtcc gcatggccat gcgggagatc cgcgcctcgt ggcggcggct
     9721 cctcttcttc ttcgtctgta tcgccgtcgg cgtggccgcg atcgtcgcga ttcgctcgct
     9781 catccagagc gtgcgcgaag ggctgacgca ggaggcgcgt gccatgacgg gcgccgacgt
     9841 ggtcgtgcga agcgatcgcc cgctcggcga cgccgtccgc gcggccgttg cccgcgagcg
     9901 cgcgagcggg agagtcggag tcgtctacga ggcggtcgag atggccacga tggtcaggcc
     9961 gtccggcgcc ccatcggccc gcatggtcga gcttcgcgcg gtccaggacg cgttccctct
    10021 gtacgggacg atgacgctca gcgagggcac ctattcgcac tcgctgctgc gtgggcacgg
    10081 cgcgctcgtt cgtcccgagc ttctcacgca gctcgggctg cgcgtgggcg acgacctcct
    10141 catcggaacg gagcggtttc agatccgcgg cgtcatcgag agcgagccgg gccgaaacct
    10201 cggagcgttc tccctgggct cccgcgtctt catcgacctg gccgatctgg cgtccacgaa
    10261 gcttctgtca ttcggcagcc gcgcgtccta cgagctgctg ctgcaggtgc ctggcccgcc
    10321 gcccgtcccc ggccgaaagg acgtgagcac ggtcatggcg tccgagttgt cggacgcgtt
    10381 cgtgaacgac ttcgtgcgtg tgcggtcgta ccggcagaac gaaggccgca tgaaccagaa
    10441 cctgacgcgc gccgagaact acctgagcct cgtcgggctc gtggtgctga ttctcggcgg
    10501 catcggcgtc tcgagcgtga cgcgggtgtt cgtccagcag aaggtgcgag cgattgctat
    10561 cctcaagtgc gtcgggagca cgacccgcca ggtgctcgcc gtctatctca cgcaggtcgt
    10621 cctgctcggc acggcaggca gcatgctcgg agtcggcatc gcgtcggcgg cgcttgccgc
    10681 cctgccggtg tttgtcggcg acctggcggc gatcttcaag gtcgactacg ggttgacggc
    10741 gggcgccgtc acccagggcc tcgcggtggg gctgctcgtc tcgctgcttt tttccatcgt
    10801 gccgctcctc gaggttcgca acgtcaagcc gtcccttctg ctgcggcagg acgtgccgcc
    10861 atcgccgggg gtagattggc tgaagtggat cgtcgcgacc gccgttgcct cgtcgctcgt
    10921 cgcggttgca tcgtggcagg ccggttcgct tcgtgtgggg ctcgtgctgt cggggggatt
    10981 tgtcgccgtg gctttcgtgc tgcacctcgc cggcgccgcc ctcatccgcg cggtacagcc
    11041 gcttcgacat tcgcggtcgt tcgcgctgcg tcaggccgtg ctgcacgtcg cccgcccggg
    11101 gaatcagacg cgcatcatcc tgctcgccgt cggcctcggg gccttcttca tcctgggtgc
    11161 gcgggcgttg caagtgaacc tgttgaagga tttctcactc caggtcggcg aaggcgcccc
    11221 ggacatgttc ctgatggaca ttcaagcgac ccagcggcag agcttgctgg cgctgatcga
    11281 ccgtgagaac ggccctgacg gcccgcccaa gctcatcccg gtcctgcgtg cgcggatcgt
    11341 cggcgtgcgg gggcgggacg tgaatctcga gagctacgag gacgtgcgcg gccggggcgg
    11401 gctgtcgcgc gagttcacgg tgacgtaccg ctcgacgctc gagcccaacg aaaagctcgt
    11461 ggacgggacg tggtgggacg agacgccggc tccggacgct gccgaggtct ccatcgagga
    11521 gggcctccgg gcgcgctacc agatccagtt gggcgacgag atgcgcttcg acgttctcgg
    11581 gcgcatcgtc accgcgcgcg tgaccagctt ccgggcggtg gactttcgtg acttccgcgc
    11641 tggcggcttc atgatcgtgt tcaggccggg gccattcgac ggcgcgccgc actcgttcat
    11701 cgcggcggtg catggaggag gggatcggcc cgcccgggcg cgcctgcagg gcctgctcgt
    11761 cgccagccat cccaacgtct cggtgatcga tttgcgcgag gcgctcgaca gcatcaagag
    11821 catcgtcgac aacgtcacgc tcgcggtgac ggtcgtgggg gcgctcgtgc tcctcagtgg
    11881 aagcctgatc ctcattggcg ccgtctcgat gacgaagttc cgccgcgtgt acgaggccgc
    11941 catcctgaaa acgctcggcg cgagcagccg gctgatcgcc gcgatgctgc tcctcgagta
    12001 cggcgtcctc ggcgctatcg ccggcaccgt cggcgcgctc ggggccatcg cgctgagttg
    12061 ggccgtcgcg cgctacgcgc tcgacatgtc gtgggaacca acgccgatcc tgaccattgc
    12121 gggcatcgtc gtgaccgcaa tcgccgtcgc cgcgatcggc gtgctcgcca gcctggacgt
    12181 gctgcggcac aagccactct cgacgctgcg tgcggagtag cggtacaatc gctatttgag
    12241 ccgggcgcga aagggtccgc aggggagttc gatccatgga cgtcgaccac tatcgtgacg
    12301 cgctgctgag gaagcgaggc gagctgctgg gtggctctgc ttcgaagccg ctgcagtgga
    12361 cgatggagaa caactccggg cgtcagggag acatggcgga tcaggcggcc ggcaacaacg
    12421 aagtccacat ccagctcaaa ctgaaacaga ccgacgccaa gattcttcaa gcgatcgaag
    12481 gcgcgctgcg ccggatcgag gagggcacct acggcgtctg ccgcgactgc ggcgagccca
    12541 tcgccgaggc ccgcctgaac gcgatcccgt ggacgcgggc gtgcatcagc tgcaaggaga
    12601 aacaggggta gccgtgtcgc cggacgagct tcgatcgctg ctgacctcgt ttaccgccga
    12661 tcggctggcg ctgctttcgc gtcacgaggc gggcgcgaag gtcgtcagcc actacgactt
    12721 caacaacgcg tatcagtacg tcatcgcccg ggaagaaacg cagctgtcgt ggctgcagag
    12781 cgcgctcgaa gagttgggtg cgtccgcgct cccgccggcg gccgccgtca ttcccgttcc
    12841 gccaggcccg aaaccggccc gccgcgtcag cgcgtcggcc ttccgcggca tcctcgatga
    12901 cgacgtccgt caccttgccg acttcgtcaa gcggtggcgg gcccccgtgg ccgcgatgac
    12961 gcaggcgagg catcgcacga tgctccaggt catccttggc gagtcggtcg agcatcagcg
    13021 cctgttcgag caggccgccg ccggactcga ggatgtgatc ggccgccgca ccacgggtgc
    13081 cgagcgcgtc ggcgcggtct tgccgacacg ctggatggag taaaaggggg tcgggagtca
    13141 ttttcggcgc gtccaagaat aatgactccc gacccccttt ttcccgtcgc gattgcgctc
    13201 ggcagcaacc tcggcgatcg ccacacgtcc ctccgcgcgg cgatcgacca actgcgcccc
    13261 ctcctccagt cgatgcgcgc gtcgagcttt cacgaaacgg cgcccgtcgg agtaggcgat
    13321 cagccgatgt tcctcaacgc agccgtcgtg ggcgagacca agctccccgc gcatgacgtg
    13381 ctcagagccc ttctggacat cgaggcgagg cacggcaggg agcgcccata tccgggggcg
    13441 cctcgaacgc tcgatctgga cttgattctg tacggcggag aggtgatcga ttcgccggaa
    13501 ctcgtcgtgc cgcacccgcg gttcaggcag cgccggttcg tcctggagcc gctgtcggaa
    13561 atagcggccg actggcgcga cccgatcacg ggccgcacgg tgtccgagct gctgctgaca
    13621 ctgagcgctt gacgcagggc cccagggctc aggcctcggg gcccagtctg atctactcgg
    13681 ccgttctcgc cgttcgccgg aagatccccg agaagccttc ctcgaagagc gtgtacac
//
LOCUS       DBNGSH010000548         1998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3619164, whole genome shotgun sequence.
ACCESSION   DBNGSH010000548 DBNGSH010000000
VERSION     DBNGSH010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3619164"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1306)
                     /locus_tag="AC16CY_12575"
     CDS             complement(38..1306)
                     /locus_tag="AC16CY_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3354674.1"
                     /translation="MRKTANFLTLGVFAVLVSTSAPGPVPALRAQEQQAPAQPPVVFR
                     GGVDLVTSDVIVRDLRNDQFVSDLKPEEFEVYEDGVKQQIVSVVLTHGGRVFNVKTPP
                     RAPIQEGIILPTNRPTNDAAGRVFLIFIDDLHLDFRSTPRTRDLIKRMLRLLIHEGDM
                     FGVVSSGYSSISEQLTYDRQILDSLISKITGGGLRPQEIIKGMQTSQGPTELRHRAHV
                     AFKTAYDLMNNLQRLQNRRKAVIYISSGYDFNPFEQSRLSEQAARANVEVSQLESDPF
                     FRNQQSQQMLAEADLVGELTELTRAANRANATFYTIDPRGLVAGPDVDEEVQTPEWNA
                     YVRDTQDSLRVLAEETGGIAVVNQNDFDKALKRIDNETSDYYVLGYYSSNPDPLKRRR
                     RVEVRTTRQGLNVWSRGEYTLRPMPPTDQR"
     gene            1389..1847
                     /gene="rpiB"
                     /locus_tag="AC16CY_12580"
     CDS             1389..1847
                     /gene="rpiB"
                     /locus_tag="AC16CY_12580"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR3354675.1"
                     /translation="MRIALSADHAGFELKQHLFAHLVRQGHDPLDLGTHSTSPSDYPD
                     AAEAVADALRDGQAERGVIVCGSGAGVSVAANKFRGIRAAVCHDTYTAHQAVEHDDLN
                     VLCLGSRVVGSALASEIVDTFLAARFSGEPRHRRRLDKLLAIEGRFAKQG"
BASE COUNT          327 a          646 c          657 g          368 t
ORIGIN      
        1 cgagtctccg tccgtcaagg acggcgtgcg tgatgtttca gcgctgatcg gtcggcggca
       61 tcggtcgcaa cgtgtactcg ccgcgcgacc agacgttgag cccctgccga gtggtgcgca
      121 cctcgacccg gcggcggcgc ttcagaggat ctggattgga cgaatagtac ccgagcacgt
      181 agtagtcgct cgtctcgttg tcgatccgct tgagcgcctt gtcgaaatcg ttctggttca
      241 cgacggcgat gccgccagtc tcctcggcga gcacgcggag gctgtcctgc gtgtcgcgca
      301 cgtaggcgtt ccactcggga gtctggactt cctcgtcgac gtccggcccg gcgacgaggc
      361 cgcgtggatc aatcgtgtag aacgtggcgt tggcgcgatt ggcggcacgc gtcaactccg
      421 tcagctctcc gacgaggtcc gcctcggcca gcatctgctg gctctgctgg ttgcggaaga
      481 acgggtcgct ctcgagctgc gacacctcga cgttcgcgcg cgctgcctgc tccgagagcc
      541 gcgactgttc gaacgggttg aagtcgtagc cgctgctgat gtagatgacg gccttccggc
      601 ggttctgcaa ccgctgcagg ttgttcatca ggtcgtacgc cgtcttgaaa gcgacgtggg
      661 cccgatgccg gagctcggtt ggcccctggg aggtctgcat gcccttgatg atctcctgcg
      721 gcctgagccc gccgccggtg atcttcgaga tcaacgagtc gaggatctgg cgatcgtagg
      781 tcaactgttc ggagatcgac gagtagcccg agctgacgac cccgaacatg tcgccttcgt
      841 gaatcagcaa ccgcagcatc ctcttgatca agtcccgggt tcggggagtg ctcctgaagt
      901 ccaggtgcag gtcgtcgatg aagatcagga acacgcggcc cgcggcgtcg ttggtcggcc
      961 ggttggtcgg cagaatgatg ccttcctgga tcggcgcccg cggaggcgtc ttcacgttga
     1021 agacccggcc gccgtgcgtc agcacgaccg agacgatctg ctgcttgacg ccgtcttcgt
     1081 agacctcgaa ctcctccggc ttcaggtcgg acacgaactg gtcgttgcgc aggtcacgga
     1141 cgatgacgtc cgaggtcacc aggtccacgc cgcctcggaa gacgactggc ggctgggcgg
     1201 gggcctgctg ttcctgtgcc cggagcgcgg gcacggggcc aggcgccgat gtcgacacca
     1261 gcaccgcgaa cacaccaagg gtcaagaagt tagcggtctt tcgcataggg ccaaagtata
     1321 ctcctttgac gaacagcccc gcgattcggc cgggtacccg acggccattc ttcgagagcc
     1381 gatatttcat gcgaatagca ctgagcgccg accacgccgg cttcgaactc aaacagcacc
     1441 tgttcgccca tctcgttcga cagggccacg atcctctgga cctcgggact cactcgactt
     1501 cgccgtccga ctaccccgat gcggccgaag cggtggccga tgcgctcaga gacggacagg
     1561 ccgagcgggg cgtgatcgtt tgtggcagcg gcgcgggcgt gtcggtggcg gccaacaagt
     1621 tccgcggcat tcgagctgcc gtttgccacg atacctacac ggcgcaccag gcggttgaac
     1681 acgacgacct caacgtgctc tgcctcgggt cgcgagtcgt gggaagcgcc ctggcgtccg
     1741 agatcgtcga cacctttctg gccgcccgct tctccggcga gccgcgacac cggcgacggc
     1801 tcgacaagct cctcgccatc gaggggcgtt tcgcgaaaca ggggtaggcg ccggtcggct
     1861 caccatggca ggcgatcagc acctcgacga cctggacgcg ctcgtcaccg agctccatcg
     1921 gagcctgccg gccggatcgg cgtccgatgc cgacgacgcc ctgacgctgg ccacgtggct
     1981 gggtcaggtg cgcacgat
//
LOCUS       DBNGSH010000549         3282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_903044, whole genome shotgun sequence.
ACCESSION   DBNGSH010000549 DBNGSH010000000
VERSION     DBNGSH010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_903044"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..708)
                     /locus_tag="AC16CY_12585"
     CDS             complement(19..708)
                     /locus_tag="AC16CY_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354676.1"
                     /translation="MPSIQLDLVWTVFAVFCGYSIYTPVVTFMWAEKGQRVFQNLRVP
                     VIVFVYFLVPCAALLLLIMAFDGIREPTWQFVAASGLVLSLSFIVMLYILTVPDWPIG
                     RFLQIDRVPVGAIRALISVNFGASLLIAIVCATIVVQAPQAIEQVGQRGQELQQLEAQ
                     TQRLDEQKDQSAERISGIRAEAVRKVIDDNVKTVRGVLAALASGVGLVGSLRALGDKK
                     KRRRRSNRDRD"
     gene            complement(775..1746)
                     /locus_tag="AC16CY_12590"
     CDS             complement(775..1746)
                     /locus_tag="AC16CY_12590"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3354677.1"
                     /translation="MAITVLVTGARGLLGQHLMRALLESGRSVRALVRSGGDRDFLPD
                     GVAMAVGDITNLAEVRRAMAGCDAVIHACSTHDYKLPPRRFWEVNVGGTHNVCTAAAE
                     RPSVKVVFTSTISTLASGGTVDGFPSAVPARKLMSLSKKAAEEEVLAHARRGLPAIVV
                     NPPYFIGPLDYRPSPFRLWAPLAVRMPIRLVPGGGFNVMSARDVARAHVWALERGRAA
                     TRYPLVGRNVSLVEYASLLNAAAGRRVVPREIPAALLRSVAVGKVFDPYAVDLITRLN
                     YVFEQDAVPIERESLEDLVSGTVRWFRDERHLAGVRVLAAYAWRRCF"
     gene            complement(1827..2624)
                     /locus_tag="AC16CY_12595"
     CDS             complement(1827..2624)
                     /locus_tag="AC16CY_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3354678.1"
                     /translation="MNRGDAETRRRILIAAGVAFAQAVRGFAGSAQTPQAAALVDDLV
                     IANHVLAMEGVLDAMGHISVRHAARPDRFLLARSMAPELVAVADIMEYDLGAQAVDAR
                     GRTSYLERFIHSEIYRSRPDVNAIVHCHTPSLIPFASSSVPLRAMYHMAAFVAEGVPV
                     FDIRKAAGVTDLLVRDAALGRALAQTLGSRSAALMRGHGAVVVAGSIPTAVGRSVYLD
                     INARAQSQAIALGGTITYVEADEAKLRMGDANEYARAWELWKRRIAR"
     gene            2685..>3282
                     /locus_tag="AC16CY_12600"
     CDS             2685..>3282
                     /locus_tag="AC16CY_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354679.1"
                     /translation="MNTRGAAHGLLALIAIIVGMLPGFSSIAGQARPPGTNGVTTSYW
                     KIPKSDNVVRMERQPSGEMRFWAHQLDTNHDGTRKALATRGLEIDVRDMTPEYVLRFC
                     GFGYDNGLKYTVIESKPGDPVFQVVVAIGFDLFRGVEHPKYGDTRWRVKAWHPDGDTL
                     RIEGRLIGWRGLLTFGQIKDDVTMTRLDVPGPACTQSPI"
BASE COUNT          580 a         1115 c         1049 g          538 t
ORIGIN      
        1 cagcgccaat ccatgacttc agtcacggtc gcgattcgat cgtcggcgcc gcttcttctt
       61 gtcgcccagc gcacgaaggc tccccaccag cccgacccct gacgccagcg cggcaaggac
      121 ccctcgcacg gtcttcacgt tgtcatcgat cactttgcga acggcctcgg ctcggatgcc
      181 ggatatcctc tccgcggact ggtctttctg ctcgtccagt ctctgggttt gcgcctccag
      241 ttgctgcagt tcctgccctc gctgtccgac ctgctcgatc gcctgcgggg cttgaacgac
      301 gatcgtggcg cacacgatgg cgatgaggag cgaggcgccg aagttgacgc tgatcagcgc
      361 ccggatcgcg ccgacgggca cccggtcgat ctgcaggaac ctgcctatcg gccagtcggg
      421 aacagtgagg atgtagagca tcacgatgaa cgacaggctc agcacgagcc cgctggctgc
      481 gacgaactgc catgtgggtt cacgaatgcc gtcgaaggcc atgatgagca ggagcagcgc
      541 cgcgcacgga acgaggaagt agacgaacac gatcaccggg actctcaggt tctggaatac
      601 gcgctgtccc ttctcggccc acatgaacgt gacgacgggc gtgtagatcg agtagccgca
      661 gaagacggcg aacacggtcc acaccaggtc gagttgtatc gaaggcatct ggcatccctc
      721 cgtcgcgcgg tccacgccgg cgaacaacag aacgactcgg acttcagtcc cgggctagaa
      781 acagcggcgc catgcgtagg ccgccagcac tcgcacgccc gcgagatgcc gctcgtcgcg
      841 gaaccatcgc acggtgccgg agacgaggtc ttccagcgac tcccgttcga tgggaacggc
      901 gtcctgctcg aacacgtagt tcaaccgcgt aatcagatcg acggcatacg ggtcgaacac
      961 cttgcccacc gccaccgatc ggagcagcgc cgccggaatc tcccgcggca cgacgcgccg
     1021 cccggcggcg gcattcagca gcgaggcata ctccaccagg ctgacgttcc tgccgacaag
     1081 cgggtagcgc gtcgcggcgc gtccgcgctc gagggcccac acgtgcgcgc gtgcgacgtc
     1141 gcgcgcgctc atgacgttga agccgccgcc aggaaccagc cggatcggca tccggacggc
     1201 cagcggcgcc cacaaccgga acggcgacgg tcgatagtcc agcggcccga tgaagtatgg
     1261 cgggttgacg acgatcgccg gcagccctcg ccgggcgtgc gccagcacct cttcctccgc
     1321 cgctttcttg ctgagactca tgagttttcg cgccggcacg gccgagggaa acccgtcgac
     1381 ggtgccgccc gacgcaaggg tggaaatcgt gctggtgaag acgactttca ccgacggccg
     1441 ttccgccgct gcggtgcaga cgttgtgcgt gccgccgacg ttgacctccc agaagcggcg
     1501 cggcgggagt ttgtagtcat gtgtgctgca ggcgtggatg acggcgtcac atccggccat
     1561 cgcccgacgc acctcggcaa ggttggtaat gtcgccaacc gccatcgcca ctccgtcagg
     1621 gagaaaatct cgatccccgc cgctccgcac gagcgcccgg acggatcgtc ctgattcgag
     1681 cagtgcccgc atcaggtgct gccccagcag gccgcgagcg ccggtcacga gcactgtgat
     1741 cgccatgact ggtcccagtt tcgtgcggct cgtccatcct gggggtgagc tgctcctggc
     1801 tcgccgcagg tcgcggctgg actcgatcat cgcgcaattc tgcgcttcca caactcccac
     1861 gcacgggcgt attcgttggc atcgcccatc cgaagcttcg cttcgtccgc ctcgacgtag
     1921 gtgatcgtgc cgccgagcgc gatcgcctgt gactgggccc tggcgttgat gtcgaggtag
     1981 acgctgcgcc ccacggcggt cgggatcgat ccggcaacga cgacggcgcc gtgcccgcgc
     2041 atgagggctg ccgatctgga gccaagcgtt tgcgcgagcg cgcgaccgag cgccgcgtcc
     2101 ctcacgagca ggtccgtcac gcctgcggct ttcctgatgt cgaacaccgg cacgccctct
     2161 gccacgaacg ccgccatgtg atacatggca cggaggggca cgctcgagct ggcgaacggg
     2221 atcagcgagg gcgtgtggca gtggacgatg gcattgacgt cgggccgcga gcggtagatc
     2281 tcgctgtgta tgaaacgctc gaggtaggag gtccggccgc gcgcgtcaac cgcctgcgcc
     2341 ccgaggtcgt actccatgat gtcggcgacg gccacgagct ccggcgccat cgaccgcgcc
     2401 agcaggaagc ggtcgggccg cgccgcatgg cgcacactga tatgccccat cgcgtcgagc
     2461 acgccctcca tggccagcac atgattggcg atgacgagat cgtcaacgag agcggccgcc
     2521 tggggcgtct gcgcggagcc ggcgaaccct cgcaccgcct gcgcaaacgc cacgccggca
     2581 gcgatcagga tccgtctccg cgtctcagcg tctccgcggt tcatcgtgcc ttctccctaa
     2641 tctatactct ttgtacttcc caccctcagg acggataccg atccatgaac acacgcggtg
     2701 cggcccatgg gctgctcgca ctgatcgcga tcatcgttgg aatgcttccg ggattctcgt
     2761 cgatcgccgg ccaggcccgg cctccaggca cgaatggcgt gacgacctcg tactggaaga
     2821 ttcccaagag tgacaacgtg gtgcgcatgg agcggcagcc cagcggggag atgcggttct
     2881 gggcgcacca actggacacg aaccatgacg gcacgcggaa ggccctggcc acgcgcggcc
     2941 tcgaaatcga cgtcagggac atgaccccgg aatacgtatt gcggttctgc ggattcggct
     3001 acgacaacgg gttgaagtac acggtgatcg agagcaagcc cggcgacccg gtctttcagg
     3061 tcgtcgtcgc catcggcttc gatctctttc gaggcgtgga gcaccccaag tacggcgata
     3121 cgcgatggcg cgtcaaggca tggcatcccg acggcgacac cctgcgcatc gagggccgcc
     3181 tgatcggctg gcgcggcctt ctgacattcg gtcagatcaa ggacgacgtc accatgaccc
     3241 ggctcgacgt gccggggccg gcctgcacgc aatcgccgat ct
//
LOCUS       DBNGSH010000550         2301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2091471, whole genome shotgun sequence.
ACCESSION   DBNGSH010000550 DBNGSH010000000
VERSION     DBNGSH010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2091471"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="AC16CY_12605"
     CDS             <1..1050
                     /locus_tag="AC16CY_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HIR3354680.1"
                     /translation="AHKLFRADFTVMTYDGAIDAVTFLGLGLFLWAGAHEVLGGRLTI
                     GGLVAFNSLLTLATVPISNLLLLWDSLQRATVLLNRLSDVFEQEPEQGHDRSRLRSVR
                     DLTGQITFRRVGFRYGGPEAPPVLQDITLDVPAGKTVAIVGRSGSGKTTLVKCLAGLL
                     EPTEGAILFDGLDLKTLNYRDLRRQIGFVLQDAYVFADTIARNIAFGESEPDMNRVKW
                     AAQMASADDFIERLPFGYDTKIGETGLALSGGQRQRIAIARALYDKPPILVFDEATSS
                     LDTESEQAVQRNMDTLLRGRTTFVIAHRLSTVRNAGLIVVLEQGRLVEQGTHDELMKR
                     QGLYYYLASQQLGIS"
     gene            1047..2297
                     /locus_tag="AC16CY_12610"
     CDS             1047..2297
                     /locus_tag="AC16CY_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354681.1"
                     /translation="MTTTKAEEPPPFLDEEPPPWAVRALATVLIALFMTALVALFVVQ
                     VPETVSASFVLEPMRGTDPVRALHPGIVSTVNVTEAQSVAEKDVLFVLTSELVGDRVS
                     ERQAVGTRLSGERGRLTNERTKYENQQRADEQERVRLEQRLVTLQRQADVKEQQLALT
                     KNIAAQREREYKEGLLSLLDANRAKLEVEQLAGELERVRTEIVDSRNTMARLAFEMES
                     RRAAFTEIERTIAEEMNTFRARKSVLDRDTLREGNALSIGAPCNGTVVKLHVRQPGAI
                     VDESDLMAELVCAGEPLQAELMVPERGMALVRVGQSVKLLYDAFPYERYGVQYGTLKW
                     LSPASTAGPRGASFRAFAELGSEAVGVQGRPRAVLPGMTGRAAVVVGRRSLASYALEP
                     LRQMRESLAAGPLAAGNAPATPSR"
BASE COUNT          367 a          745 c          820 g          369 t
ORIGIN      
        1 gcgcacaagc tgtttcgtgc cgacttcacg gtcatgacct acgacggggc catcgacgcc
       61 gtgaccttcc tgggtcttgg cctgttcctc tgggcgggtg cgcacgaggt cctcggcgga
      121 cggctgacga tcggcggtct cgtggcgttc aactcgctcc tcaccctcgc cacggttccg
      181 atctcgaacc tgcttctgct ctgggacagc cttcagcggg cgacggtgct gctcaatcgg
      241 ctcagcgacg tgttcgagca ggagccggag caggggcacg accgttcgcg gctgcgctct
      301 gtccgcgacc tgacgggaca gattacgttc cgtcgcgtgg gtttccggta cggtggcccg
      361 gaggcgccgc cggtcctcca ggacatcacg ctcgacgtgc ccgcagggaa gaccgtggcg
      421 atcgtggggc gcagcgggtc gggaaagacc acgctggtca agtgcctggc cgggttgctc
      481 gagccgaccg aaggcgccat cctgttcgac ggcctcgacc tgaagacgct gaactaccgc
      541 gacctgcggc gtcagatcgg cttcgtgctg caggacgcct acgtctttgc cgacaccatc
      601 gcgcgcaaca tcgcgttcgg tgagagcgag cccgacatga atcgggtcaa gtgggccgcg
      661 cagatggcga gcgccgacga cttcatcgag cggctgccgt ttggctacga cacgaaaatc
      721 ggcgagacgg ggctggcgtt gtcgggcggg cagcgccagc ggatcgccat cgcgcgcgcc
      781 ctgtatgaca agccgccgat tctcgtgttc gacgaggcca cgagctcgct cgacaccgaa
      841 tcggagcagg ccgtgcagcg gaacatggat acgctccttc gcgggcgcac cacgttcgtc
      901 atcgcccacc ggctcagtac cgttcgaaac gcggggctca tcgtcgtgct cgagcagggc
      961 cggctcgtcg agcaggggac gcacgacgag ctgatgaaac gtcagggcct ctattactat
     1021 ctggcgagcc agcagttggg gatttcatga cgacgacgaa ggcggaggaa ccgccaccgt
     1081 tcctggacga ggagccgcct ccctgggccg tgcgcgcgct ggccactgtg ctgatcgcgc
     1141 tgttcatgac ggcgctcgtg gccttgttcg tcgtgcaggt gcctgaaacg gtgtcggcgt
     1201 cgttcgtgct cgagccgatg cgcggcaccg acccggtgcg cgcgctccac cccgggatcg
     1261 tctcgacggt caacgtgacg gaagcccagt cggtcgccga gaaggacgtc ctcttcgtcc
     1321 tgacctccga gctggtcggc gatcgcgtct cggagcgtca ggccgtcggc acgcggctgt
     1381 cgggtgagcg cggcaggctc accaacgagc ggacgaagta cgagaaccag cagcgcgccg
     1441 acgaacagga gcgcgtccgg ctggaacagc ggctcgtgac gctgcagcgg caggccgacg
     1501 tcaaggagca gcagctggcg ctgacgaaga acattgcggc ccagcgtgag cgcgagtaca
     1561 aggaggggct gctgagcctg ctcgacgcca atcgcgcgaa gctcgaggtg gaacagctcg
     1621 cgggcgagct ggagcgcgtg cgcaccgaga tcgtcgattc gcgcaacacg atggcgcggc
     1681 tggcgttcga aatggagtcc cggcgcgccg cgttcacgga gatcgagcgc acgatcgcgg
     1741 aagagatgaa cacattcagg gcgcgcaagt cggtgctcga tcgcgacacg ctgcgcgagg
     1801 gcaacgcact gtcgatcggg gcgccctgca acggcacggt cgtgaagctg cacgtccggc
     1861 agccgggcgc catcgtggac gagagcgatc tgatggccga gctcgtgtgc gcgggcgagc
     1921 ctcttcaagc agagctgatg gtgcccgaac gcgggatggc gctcgtgcgg gtggggcagt
     1981 ccgtgaagct gttgtacgac gcctttccct acgagcggta cggcgtgcag tacggcacgt
     2041 tgaagtggct gagtcccgcg tccaccgccg ggccgcgggg cgcctcgttt cgagccttcg
     2101 ccgagctcgg ctcggaggcg gtcggcgtgc agggacggcc ccgtgcagtg cttccgggca
     2161 tgacgggccg cgccgcggtc gtcgtcggcc gccggtctct cgccagttac gcgctcgaac
     2221 cgttgagaca gatgcgcgag tcgctggcgg ctggtcccct ggccgccggg aacgcgccgg
     2281 ccacgccctc ccgctgaccc g
//
LOCUS       DBNGSH010000551         9941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1241457, whole genome shotgun sequence.
ACCESSION   DBNGSH010000551 DBNGSH010000000
VERSION     DBNGSH010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1241457"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="AC16CY_12615"
     CDS             <1..194
                     /locus_tag="AC16CY_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end joining protein Ku"
                     /protein_id="HIR3354682.1"
                     /translation="AKIAGEEIVAPEVQEPPKVVDLMEALRKSLDSVSADKKKLAKAA
                     LGKSAAKAKAANGRKRKAS"
     gene            complement(776..1543)
                     /locus_tag="AC16CY_12620"
     CDS             complement(776..1543)
                     /locus_tag="AC16CY_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354683.1"
                     /translation="MSDSTEAARVLSELSTFGPVLAVKVYRIALDPVPAVPLLPCLDH
                     EAMLHEGVAPHVAAYLQEASSGDLHEIVLTPAARRIDIDAVSTWGECSPESQARLSNV
                     LARTAPGFRIAIAGPSWWRGDRRVAAACRAQVSLRDVLLGADMAGVRAGIDRLLTISA
                     LMEKQSRVASWGARTVTGPLLAAAGVMSYFVVGSLAGQLGDAGVTTLRYALISLLGAC
                     FLYYGLKAVQLTGMSNRVWKRTAEYNLILSERRRLGK"
     gene            complement(1679..2449)
                     /locus_tag="AC16CY_12625"
     CDS             complement(1679..2449)
                     /locus_tag="AC16CY_12625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3354684.1"
                     /translation="MTFTGFIGTVCMFPLRAIIGVSVALGVHPNLLTLTGLLINVVAA
                     CDLGLDRFRIAGVIMIVANIFDFIDGKVAHITNTVSRFGAFWDSTLDRFSDIVLFLGL
                     IYLYADLHRTDYVMITALAMMFTIMTSYARARAESLIEKCKVGFMERPERIVLFMIGA
                     FTNRMAAVMWVILILSIVTVANRIHYTYLALNNKPIPAGSNFVTAVFWRAFFWRDERT
                     TLPYDLWVIAILAFVWLTPPDWLGDPTATGMGIWGLGH"
     gene            2503..4317
                     /gene="uvrC"
                     /locus_tag="AC16CY_12630"
     CDS             2503..4317
                     /gene="uvrC"
                     /locus_tag="AC16CY_12630"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3354685.1"
                     /translation="MPVQDLKDQIARLPEQPGVYLWGNAAGQTIYVGKARSLRDRVRS
                     YLGAFGMSPRHDALLDDIARLEVIVTDSVMEALALETNLIKQRMPKYNILLRDDKNYP
                     YLRLTTSEPFPRILVARSVDKTGDFYAGPFLPAKLARRTMTLAHRLFGIRSCNEVITG
                     QRGRPCLEYDIKRCVAPCVREICTTNQYAAAVADTKLLLEGRNDELVELLRDRMTEAS
                     GRQYYEEAAHLRDAMRTVQALRDREQKIATPAFGDRDVFGLKVGPSGVAIQVFQVRGG
                     RVVERVDLGTDPGAPAAGEADVLHAALAQFYEHRAAAPEINLPVAIEDADVMEEWLSP
                     RAGRRVRILVPLRGDKRALVELATRNAELSYRSRFNETTAAHFDALETLRSQLGLPAT
                     PRRIDCFDISTIQGSETVASMVVCEDGRMKKAEYRKFRIKEEPHRGSDDFAAMREVVQ
                     RRYRAVLERGGPFPDLIVVDGGRGQLSAAYEALEQIGLANLVVVGIAKKEELLFTRNS
                     AEPLALAEDDPALLLVERIRDEAHRFAVTFHRKARSMRDLRSELDQIPGVGPRRRRAL
                     LGAFGSLAGVRRATREELAAIVGAKTAGAVIDYFARSA"
     gene            4456..5577
                     /locus_tag="AC16CY_12635"
     CDS             4456..5577
                     /locus_tag="AC16CY_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3354686.1"
                     /translation="MRNCGWRWLLVAFVFTLTWQGSASQAGAEQTPARSAREVYQSSC
                     AACHGIDGRGAPAAQVGFDTPLPDFTDCSFATREPDADWFAIAHDGGPVRAFHRRMPA
                     FGEAIDAGEIQQSIDYIRGFCGEPAWPRGDLNLPRPLVTEKAFPEDEAVFSTVVGTGA
                     AGTVTNEFLYERRFGARNQIEIAVPVAMAESSGGAWSRGLGDVAVALKRVLFHSLNSG
                     SILSATGELVLPTGDDAEGLGKGVGVVEPFLTYGQLFPRNAFLQAQAGAELPFDRAVA
                     SNEAFWRVAFGRTYEQGRFGRAWSPMIELLGARELVAGEPATWDLVPQIQVSLSKRQH
                     VLINGGVRIPLNERGERHVSVLTYVLWDWFDGGLFDGWR"
     gene            5577..7196
                     /locus_tag="AC16CY_12640"
     CDS             5577..7196
                     /locus_tag="AC16CY_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354687.1"
                     /translation="MRRLAVIAASLAWLLAALVQSPRAQEPQAGPHAGVAFDTSDNCL
                     ACHNGLTASSGEDVSIGVAWRASMMANASRDPYWLAGVRRETIDHPTKRSVIEDECSI
                     CHMPMATTLARAAGRPGEVFRLVPGAGDTEEHRLAADGVSCTLCHQISPDRLGTRESF
                     VGGFTIAPPENGERRMFGPFDVTAGRAALMRSATGVRPTEGEHIRRSEVCATCHTLIT
                     EAFGPSGEVIGSLPEQMPYIEWRHSALAEERSCQSCHMPAAASAPVASVVGEPRERLA
                     RHTFLGGNAFMLRMLNRFRAELGVTAPSQELDAAAHATLRQLAEDTASVSIARAALVG
                     GRLEADVVVSNRTGHKLPTGYPSRRAWLHVVVRDARGRTLFESGATQPSGAIAGNDND
                     ADAARFEPHYEEVRTADQVQIYEAIMGDRTGGVTTGLLQATSYLKDNRLLPRGFDKAT
                     ATSEIAVRGPAAADRNFTGDGDRVTYVIDLADGSGPFEMSVELRYQTIAFRWADNLRR
                     YNAAEPRRFVSFYDAMAESSTAVLARASARF"
     gene            complement(7413..8147)
                     /locus_tag="AC16CY_12645"
     CDS             complement(7413..8147)
                     /locus_tag="AC16CY_12645"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3354688.1"
                     /translation="MFDLIAGRVKHIPSHSGMPVLVSGTAQAAAIGLLVVLPALFVAD
                     QLPQVPTMLAFVAAPPPPPPPPPPPAPAKPKPPSEDRPATIDRQLVAPLEAPSKILPL
                     PDDEGVDFGVPGGVEGGIPGGVIGGVVGGLPEAPPPPPPARERGPVRVGGQINPPQLV
                     RRVEPDYPKFAVDARLHGVVILEAIVDEDGKVVDVTVLRSAHKLLDREAEMAVRQWRY
                     SPLVLNGTPVRFVLSVTLVFRLQPAE"
     gene            8394..8891
                     /locus_tag="AC16CY_12650"
     CDS             8394..8891
                     /locus_tag="AC16CY_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3354689.1"
                     /translation="MFTHKELARVEPRAFVRRMLRDFDRFFEEGWTPFTRAGGTFGEF
                     GWVPALEVVEKENRLVARLDLPGVKKEEVSVSITDEALTIEGERKFEEEEKKNNWYRS
                     ERTYGKFVRTVPLPAGVKTADIKATFLNGVLEIVVPLPIEAAAAPPRKVEIAGGEEKK
                     TVRAA"
     gene            8987..>9941
                     /locus_tag="AC16CY_12655"
     CDS             8987..>9941
                     /locus_tag="AC16CY_12655"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3354690.1"
                     /translation="MTKGTVLIVDDEELVRWSLRERFIRDGYTVVEAGTAAGAIEHLT
                     PATDLVLLDNRLPDGDGLTTLRRIKEIAPDTPVILMTAFSTVENAVEAMKHGAYHYLN
                     KPFNLDDVSAMVENALETSRLRREVRLLRGSQSREFGFDAIVGDSPAMVAAKALLERI
                     AASPATTVLLTGETGTGKDLAAKAIHYNSERAAKPFLNITCSALPEQLLESELFGHER
                     GAFTDARQQKRGLFETADGGTIFLDEIGEMTPGLQAKLLRFLEDRTFKRIGGLADIRV
                     DVRVVAATNRDLEDEVSAGKFREDLFYRLQVMPIVLPPLRER"
BASE COUNT         1666 a         3290 c         3431 g         1554 t
ORIGIN      
        1 acgccaagat cgccggcgag gagatcgtcg cgcccgaggt gcaggagccg ccgaaggtcg
       61 tcgacctgat ggaggcgctg cgcaagagcc tcgactcggt gagcgccgac aagaagaagc
      121 tggcgaaggc cgcgctgggg aagtcggcgg cgaaggcgaa ggcagcgaac ggaagaaagc
      181 gcaaggccag ttagccgttc ggaacccgca acgccacggc caagaccctc cggccgccga
      241 ttgattgagt gggtgtcgcg ccgtcccggc tgacgtgtcg cttctgcgcg ccgggtcgcg
      301 gcgcccggtc accggcatct acggcgcgcg tctatcggtt cgtcggcgct ccgccgtcgc
      361 aatcgcctgc cgcccttgct cctccttcgc gcctctattc gccggcgatg ccgatggcga
      421 cctgtcgcga gacgggcttg cgacacgccg tcgcttcagg acccttcagg cgggccggcg
      481 gcttgtccgc tcgagacgca gtctgtcgcc acacctgcct gtcgggtcgg ccggcgcctg
      541 cgcaagcgcc actcagggcg aagcgggaaa tcggctgacg cgagccttcc gcggcgagtt
      601 cggcttccgc gttctggttg tgccgagtga ccacggggtt tcgtgccgtg taacagacgt
      661 cgtgtcgaat cagctcctac tcgccgcgtc agcgaaggag cgacgagcag aagccgattt
      721 ccggcgtgca gcgccggcgc cgccggaccg acaggcaggt gtggcgacgg atcggtcact
      781 ttccgaggcg tcgacgttct gacaggatca ggttgtactc cgccgtgcgc ttccacacgc
      841 ggttcgacat cccggtcagc tggaccgcct tcaggccgta gtacagaaag caggcgccca
      901 gcaggctgat gagcgcgtag cgcagcgtcg tcacgcccgc gtcgccgagc tgtccagcca
      961 gcgacccgac cacgaagtac gacatcaccc cggccgcggc cagcagaggt cccgtcacgg
     1021 tgcgcgcgcc ccacgacgcg acgcgcgact gtttctccat cagcgccgag atcgtcagca
     1081 ggcggtcgat tcctgcgcgg acgccggcca tgtcggcgcc gagcagcacg tctctcaagc
     1141 tcacctgggc gcggcatgcc gcggccacgc gcctgtcgcc gcgccaccac gacggtcccg
     1201 cgatcgcgat tcgaaaacct ggagccgtcc tggcgagcac gttcgagagc cgcgcctgcg
     1261 actccggcga gcactcgccc caggtggaca cggcgtcgat gtcgatgcgg cgcgccgccg
     1321 gcgtgagcac gatctcgtgg agatcgccgg atgacgcctc ctgcagatac gccgcgacgt
     1381 gcggcgccac gccttcgtgg agcatggcct cgtgatcgag gcaggggagc agcgggacgg
     1441 ccgggacggg atcgagagcg atgcgataga ccttgacggc gagaacgggc ccgaacgtgc
     1501 tcagctcgct cagcacgcgg gcggcttcag tcgaatcaga catcgatcgc gccccggcca
     1561 gcggtcccgt ctagtggacc gtctgcgctg aagtggtacc acttacaacc agccccggac
     1621 tgcggtttcc cggccgcgac aacggagcgg ccggtagtga tcgacgaagg acataccgtt
     1681 agtggcccaa gccccagatc cccatgcccg tcgccgtcgg atcgccaagc cagtccggag
     1741 gcgtcagcca cacgaaggcc agaatcgcga tcacccacag gtcgtagggg agcgtcgtcc
     1801 gctcgtcgcg ccagaagaac gcccgccaga acaccgccgt gacgaagttc gagccggccg
     1861 gtatcggctt gttgttcagt gcgagatagg tgtagtgaat cctgtttgcc accgtcacga
     1921 tcgacaggat gaggatgacc cacatcacgg cggccatgcg gttggtgaac gccccgatca
     1981 tgaacaggac gatccgctcg ggccgttcca tgaacccgac cttgcacttt tcgatgagcg
     2041 actcggccct ggcgcgggca tagctcgtca tgatcgtaaa catcatggcg agcgccgtga
     2101 tcatcacgta gtccgtgcgg tggagatcgg cgtagaggta gatgagaccg aggaacagga
     2161 cgatgtcgga gaagcgatcg agcgtcgagt cccagaacgc gccgaagcgc gagacggtgt
     2221 tggtgatgtg cgccaccttg ccgtcgatga agtcgaagat gttggcgacg atcatgatca
     2281 cgccggcgat gcggaaccga tccagcccga ggtcgcaggc ggcgacgacg ttgatcagca
     2341 ggccggtcag cgtgagcagg ttggggtgga cgccgagggc cacgctcacg ccgatgatgg
     2401 cgcggagcgg gaacatgcac acggtgccga tgaagccggt gaacgtcatt cgttggtaca
     2461 attgggggtt cttcgtcgcg gatggcctcg gcatagtagc ccatgcccgt tcaggacctc
     2521 aaggatcaga tcgcccgcct gcccgaacag cccggtgtgt acctgtgggg caacgccgct
     2581 ggccagacca tctatgtcgg gaaggcgcgc tcgctgcggg accgggtccg cagctacctg
     2641 ggcgccttcg gcatgagccc gcgccacgac gcgctgctcg acgacatcgc ccgcctcgag
     2701 gtcatcgtga ccgactcggt gatggaagcg ctggcgctcg agacgaacct gatcaagcag
     2761 cggatgccga aatacaacat cctcctgcgc gacgacaaga actaccccta ccttcggctg
     2821 accacgagcg agccgttccc caggattctg gtggcgcgca gcgtggacaa gaccggcgac
     2881 ttctatgcgg gaccctttct tccggcaaag ctcgcccgca ggacgatgac cctggcgcac
     2941 cggctgttcg gcatccggtc ctgcaacgag gtgatcacgg ggcagcgcgg ccgcccgtgt
     3001 ctcgagtacg acatcaagcg ctgcgtcgcg ccgtgcgtca gggagatctg tacgacgaat
     3061 cagtacgccg ctgccgtcgc cgacacgaag ctgctgctcg aggggcggaa cgacgagctg
     3121 gtcgagcttc tgcgcgatcg catgacggag gcgtcggggc ggcagtacta cgaagaggcg
     3181 gcccacctgc gcgacgcgat gcgcacggtg caggcccttc gcgaccggga gcagaagatc
     3241 gcgacgcccg ccttcggcga tcgcgacgtc ttcggactca aagtgggccc gagcggcgtg
     3301 gcgatccagg tgttccaggt ccgcggcggg cgcgtcgtcg agcgggtcga tctgggcacc
     3361 gaccccgggg cgccggccgc cggcgaggcc gacgtcctgc acgccgcgct cgcccagttc
     3421 tacgagcacc gcgcggccgc gcccgaaatc aacctgcccg tggccatcga ggacgccgac
     3481 gtgatggaag agtggctgtc gcccagggcc ggacgccgcg tgcggatcct cgtaccgctg
     3541 cgcggcgaca agcgcgcgct cgtcgagctg gcgacgcgaa acgcggagct ctcgtaccgc
     3601 tccaggttca acgagaccac ggcggcgcac ttcgacgcgc tggagacgct ccgcagccag
     3661 ctcgggttgc cggcgacgcc gcgccggatc gactgcttcg acatttcgac gattcagggg
     3721 agcgaaaccg tcgcctcgat ggtggtgtgc gaggacgggc gcatgaagaa ggccgagtac
     3781 cggaagttca ggatcaagga ggagccgcat cgcggctcgg acgattttgc ggccatgcgc
     3841 gaggtcgtgc agcggcggta tcgggccgtg ctcgagaggg gagggccttt ccccgatctg
     3901 atcgtggttg acggcggccg cggccagctg tcggctgcct acgaggcgct cgagcagatc
     3961 gggctcgcga atctcgtggt ggtcggcatc gcgaagaagg aggagctgct gttcacgcgc
     4021 aacagcgccg agcctctcgc gctggccgag gacgatcccg ccctgctgct cgtggagcgg
     4081 atccgcgacg aggcccatcg cttcgcggtg accttccacc ggaaggcgcg ctcgatgcgc
     4141 gatctgcgct ccgagctcga tcagattccc ggcgtcggac cgcgccgccg gcgggcgctg
     4201 ctgggcgcct tcgggagcct cgccggcgtc aggcgggcga cgcgcgaaga gctcgcggcc
     4261 atcgtcggcg cgaagacggc cggggcggtg atcgactact ttgcgaggtc tgcgtaacgc
     4321 ccgccttcgc cgctgcgcgg cttcggcgcg gctagcgcac cctcgcacct tcgcaccctc
     4381 gcaccgttag attgagtctc agttcgattc tcaggttcgg cgcctgcgcg cgcgccgtgc
     4441 tagggtcgcc acgcgatgcg caattgcggt tggcggtggc tgctcgtggc gttcgtgttc
     4501 accctgacgt ggcaggggag cgcgagccag gcgggcgcgg agcagacacc cgcgcgctcg
     4561 gcgcgcgagg tctatcaatc gtcctgcgct gcctgccatg ggatcgacgg tcgtggcgcc
     4621 cccgcggcgc aggtcggctt cgacacgccg cttcccgatt tcaccgactg cagcttcgcc
     4681 acccgcgaac ccgatgccga ctggttcgcg atcgcgcacg acggcgggcc cgttcgcgcc
     4741 ttccaccgcc ggatgcccgc cttcggcgag gcgattgacg ctggcgagat ccagcaatcc
     4801 attgactaca ttcgcgggtt ttgcggcgag ccggcgtggc ctcgaggcga tctcaacctg
     4861 cccagaccgc tggtgaccga gaaagccttc cccgaagacg aagccgtatt cagcacggtc
     4921 gtcgggaccg gggccgccgg gacggtgacg aacgagtttc tctacgagcg gcgtttcggc
     4981 gcgcgcaatc agatcgaaat cgccgtgccg gtggcgatgg cggaaagctc gggcggcgcg
     5041 tggagccgtg ggctcggcga cgtggccgtg gcgctcaaac gcgtgctctt tcacagcctg
     5101 aacagcggga gcatcctgag cgcgaccggc gagctcgtgc tgccgacggg cgacgatgcc
     5161 gaagggctgg gtaagggcgt cggcgtcgtc gagccgttcc tcacctacgg gcagctgttt
     5221 ccacgaaacg cgttcctgca ggctcaagcc ggagccgaac tgccgttcga ccgggccgtc
     5281 gcgtccaacg aggcgttctg gcgtgtcgcc ttcgggcgga cctacgagca aggccggttc
     5341 ggccgcgcct ggtcgccgat gatcgagctg ctcggcgcgc gtgagctcgt ggcgggcgag
     5401 cccgccacgt gggatctcgt accgcagatc caggtctcgt tgagcaagcg gcagcacgtc
     5461 ctgatcaacg gcggcgtgcg catcccgctc aacgagcgcg gagagcgtca cgtctcggtc
     5521 ctgacctacg tgttgtggga ctggttcgac ggcggtctct tcgacgggtg gcggtgatgc
     5581 gccgtctcgc tgtcatcgcg gcgtccctcg catggctcct ggcggcgctc gtgcagtcgc
     5641 cccgcgcgca ggagccgcag gccggtccac acgccggcgt tgccttcgac acatccgaca
     5701 actgcctcgc ctgccacaac ggcctcacgg cctcgtcagg cgaggacgtg tcgatcggcg
     5761 tcgcatggcg tgcgtcgatg atggccaatg cctcgcgcga cccgtactgg ctggccggcg
     5821 tgcgacggga aacgatcgat caccctacga aaagaagtgt catcgaggac gagtgttcca
     5881 tctgccacat gccaatggcg acgacgctgg cgcgggcagc gggccggccg ggtgaggtgt
     5941 ttcgactcgt gccgggcgcg ggcgacaccg aagagcaccg cctggccgcc gacggggtgt
     6001 cgtgcacgtt gtgccatcag atctctccgg atcggctcgg cacgcgcgag agcttcgtcg
     6061 gcggcttcac catcgcaccg cccgagaacg gcgagcggcg gatgtttggt cccttcgacg
     6121 tgacggccgg gcgcgccgcg ctgatgcgat cggccacggg cgtgcggccg accgaagggg
     6181 agcacatccg ccgctcggag gtgtgcgcca cctgccacac gctgatcacg gaggcattcg
     6241 gtccttcagg cgaggtgatc ggctcgctgc cggagcagat gccgtacatc gaatggcgcc
     6301 acagcgcgct ggcggaggaa cgaagctgcc agtcctgcca catgccggcg gcggcctccg
     6361 cgccggtcgc atcagtggtg ggggagccgc gcgagcggct ggcgcgccat accttcctcg
     6421 gcggcaacgc gttcatgctg cggatgctga accgctttcg cgccgagctc ggcgtgaccg
     6481 ctccgtcgca ggagctcgac gccgcggcgc acgccaccct tcgacagctc gcggaggaca
     6541 cggcctcggt ctcgatcgcg cgcgcggccc tggtcggcgg ccgcctcgag gccgacgtcg
     6601 tcgtgagcaa ccgcaccggc cacaagcttc ccaccggtta tccgtcgagg cgtgcctggt
     6661 tgcatgtcgt cgtgcgcgat gcgagggggc ggacgctctt cgagtccggc gcgacccagc
     6721 cgagcggcgc catcgccggt aacgacaacg acgccgatgc cgcacgcttc gagccgcact
     6781 acgaggaggt ccgaacagcc gatcaggtgc agatctacga agccatcatg ggcgatcgga
     6841 ctggaggcgt gacgacgggc ctgctgcagg cgaccagcta cctcaaggac aaccgcctcc
     6901 tgcctcgcgg gttcgacaag gcaacggcca cgagcgagat cgcggtgcgc ggccccgcag
     6961 cggccgatcg gaacttcact ggagacggcg atcgcgtcac gtacgttatc gacctcgcgg
     7021 atgggtcggg gccgttcgag atgtccgtcg aattgcgcta ccagacgatc gctttcagat
     7081 gggccgacaa cctgcggagg tacaacgccg ccgagccgcg ccgcttcgtg tcgttctacg
     7141 acgcgatggc ggagtcgtcc accgcagtcc tcgcccgggc gagcgcgcgt ttctgacgct
     7201 actgcggtcg aaactcgtcc gcctcccagt gtgggcaccg ctcacatcga tcgtcggttg
     7261 ccggctgacg ctcgcctgtc cgcacgcaaa cccaggtggt ttccggctga agcgactcgt
     7321 cgacgcccgc aagcctgacg ccggggcggc tccacttgca ctcccacgcc ggctgcttcg
     7381 tagcaatggc ggtcttaatc atggaacacc cctcattccg ctggctggag gcggaacacc
     7441 aacgtcacgc tgagcacgaa gcgcacgggc gtgccgttga gcaccagcgg cgaatagcgc
     7501 cactgccgca cggccatttc tgcttcccga tcgagcagct tgtgcgccga cctcaacacc
     7561 gtcacgtcca cgaccttgcc gtcctcgtcc acgatggcct cgaggatgac gacaccgtgg
     7621 agtcgcgcgt cgacggcgaa cttcggatag tcgggttcga cccgtcgcac cagctgcgga
     7681 ggattgattt gccctccgac acgaacgggc ccgcgttcac gcgcgggcgg agggggaggc
     7741 ggcgcctcgg gcagtccgcc cacgactccg ccgatcacgc cgccgggaat cccgccttcc
     7801 actcctccgg gaacgccgaa gtcgacccct tcatcgtcgg gcaggggaag aatcttcgac
     7861 ggcgcttcga gaggcgcgac cagttgcctg tcaatggtgg caggtcgatc ctcggagggc
     7921 ggctttggtt tggcgggcgc cggcggcgga ggcggcggag ggggcggagg cggcgcggcc
     7981 acgaacgcca gcatcgtcgg gacctgcggc agctggtcag cgacgaacag cgcaggcagg
     8041 acgacaagca gaccaatcgc ggcggcctgc gccgtacctg acaccagcac cggcatgccc
     8101 gagtggctgg ggatgtgctt cacgcgtcct gcgatcagat cgaacatggc cggctcctcg
     8161 acggcagagt gtgcaagggc ctcgccagtt gtgcggatcg ctcgccttgt gtgcttccgt
     8221 cgtgtgtcgc ccgcacctgc tctcgcctgg tcgggaataa ttcgcggctc cgcgcggaaa
     8281 gccccacgcg cctggtccct ggtggagagg cgcggcccga aactccgccg gagtcgcgat
     8341 tcgcgcgagg cacggccgtt gctcaaacca cttcggatag caggaggtgc cccatgttta
     8401 cccacaagga attggcacgt gtcgagccca gggccttcgt gcgccgcatg ctgcgcgact
     8461 tcgaccggtt cttcgaggag gggtggaccc cgtttaccag agccggaggg acgttcggtg
     8521 aattcggatg ggtgccggcg ctcgaggtcg tcgagaagga gaatcgactg gtcgcccgtc
     8581 tcgatctgcc cggagtgaag aaggaagaag tctcggtctc gatcaccgac gaggcgctga
     8641 cgatcgaagg cgagcggaaa ttcgaggagg aagagaagaa gaacaactgg taccgaagcg
     8701 aacgcaccta cgggaagttc gtccgcaccg tcccgctgcc tgcgggcgtc aagacggcgg
     8761 acatcaaggc gacgttcctc aacggcgtcc tcgaaatcgt cgttccgctg cccatcgaag
     8821 cagcggcggc gccgccacgt aaggtggaga tcgcgggagg tgaggagaag aagacggtca
     8881 gggcggccta gaagttgctc tacggacgat cagagggccg gcggggccgt gacccgtggg
     8941 aaaacttccc gccacgcggg gattctttcc caaccggtgc atgacgatga caaaaggaac
     9001 cgtgctgatc gttgacgatg aagagttggt ccgctggtcg cttcgggagc ggttcattcg
     9061 ggacgggtac acggtcgtcg aggccgggac ggccgccggc gccatcgaac acctgacgcc
     9121 ggcgaccgat ctcgttctcc tggacaatcg ccttcctgac ggcgacggct tgaccacgct
     9181 ccggcgcatc aaggagatcg cgccggacac gccggtcatc ctcatgaccg cgttctccac
     9241 cgtggagaac gccgtcgaag cgatgaagca cggcgcgtat cactacctga acaagccgtt
     9301 caacctcgac gacgtgtcgg cgatggttga aaacgcgctc gagacgagcc gcctgcgccg
     9361 cgaggtccgc ctgctgcgcg gcagccagag ccgcgagttc ggcttcgatg cgatcgttgg
     9421 cgactcgccg gcgatggttg ccgcaaaggc gctcctcgag cgcatcgccg ccagccccgc
     9481 gacgacggtc ctgctgacgg gcgagaccgg aaccggcaag gacctcgcgg ccaaggcgat
     9541 tcactacaac agcgagcggg cggcgaagcc gtttctcaac atcacctgct cggcgcttcc
     9601 cgagcagctg ctcgaaagcg agctgttcgg gcacgagcgg ggcgcgttca ccgacgcgag
     9661 acagcagaag cggggtctct tcgagacggc tgacggcggg acgatctttc tcgacgagat
     9721 cggcgagatg acgcccggcc tccaggccaa gctcctgagg tttctcgagg atcggacatt
     9781 caagcgtatc ggggggctgg ccgacatccg cgtggacgtg cgcgtcgtcg ccgcgaccaa
     9841 tcgcgacctg gaggacgagg tctcggccgg aaagttccgt gaagatctct tctaccgtct
     9901 gcaggtgatg cccatcgtgc tgccgccgct gcgggagcgg t
//
LOCUS       DBNGSH010000552         4455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1751960, whole genome shotgun sequence.
ACCESSION   DBNGSH010000552 DBNGSH010000000
VERSION     DBNGSH010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1751960"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="AC16CY_12660"
     CDS             <1..1061
                     /locus_tag="AC16CY_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354691.1"
                     /translation="TALVFDSSVEGVKHKAHTTGELHDAVRSLGASPLNCVYITQDRG
                     FEREYREHCSSIGFDPVLAILNHDYWVWYAFAQYETDGERIFEQRLEAFRSRPAQRAR
                     RFLSLNRTAREAKLIFLLSLLRDGLWDKGFVSFGGFQRPDQPKGEKPRPTPEQLARSI
                     PGFDDKVAELAPFLDALDARGRVLLGMERHGWTRLELWNAGLAADLAEYDQSWFSVAT
                     ETEMRPRLSRITEKSVKPLANFHPMLVLGNPGSLQMVRSYGFVTFEEIFDESYDEELD
                     PRRRFDMVYGELTRLCRLDEKELFAMERRVEDKLIFNARWGFTEFPGAYRRQRDTALV
                     DEILAAVSRTPLPPAHGT"
     gene            1102..1431
                     /locus_tag="AC16CY_12665"
     CDS             1102..1431
                     /locus_tag="AC16CY_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3354692.1"
                     /translation="MPRDAGLPPGTLMMLVLRVLTTQPLHGYAIAQRLHQLSREELSV
                     EEGSLYPALQKLLVKGWVRAAWRESESGRQVREYRITASGRRQLDAEMAEYRRTASAI
                     GWLLDEA"
     gene            1437..4022
                     /locus_tag="AC16CY_12670"
     CDS             1437..4022
                     /locus_tag="AC16CY_12670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354693.1"
                     /translation="MGIIGEWIRRLGYLLRRGAREEELRREMEAHRAMLQEPRAFGNT
                     LRLREEARDAWGWGWLDDLARDTRLAWRTLRRSPAFALTAIVTLALGIGATGAIFTLV
                     RSVLLRPLPFPDPDALYVISYASPGPAWLYPGMSDRGYLDFRGANRTLESVATFSAAE
                     STMTGAGNATRVSGAAVTTDFFRVLGVDAAIGRTFGPEDDQSGDGRVVLIADGLWRRH
                     FGADPLFVNKTVVLNGVPHRVIGVLPPGFSYPADAAYWIPLTVRINPNLGFMRPVIGR
                     VKAGVTGEQAAADLDVWVRSLPLDPNRPRDLVAQVTPLHEAMVRDVRLPVLVFGSAVV
                     LVLVIVCANVMHLLLMRAVSRRQEIATRLALGGSRVRVVRQFLAEGALLSLAGGSVGA
                     GVAFLGGPALLSLIPPGRLPVDIAVRADGWVLAFTAGLSVCTALVVGLAPIVQISGDS
                     PYVALRGGTASATRQSHRLRHALVISELALTLVLLVGAGLLVRSVIGLRSVPLGFTPG
                     RVMTMTIDLPVTRYPKAEQATTLHSRLLQSLSASPGVLSVAAVNWLPLGDMVSWGDVQ
                     AEDRPDLAGKYNATKVAVSPDYFRTIGIRLLRGRPFTEADLPGRPPVAIVSESVARRL
                     WPGVDPSGRRMALVDRPKPEDWLTVVGVVEDVRQGGFRSAPTHAVYQPYVQVTNRYFV
                     GYMTFLVRTHGDPLQVAPTMRTALARIDEDEAPRSLATLETVVDRTVAEPQFHARVLT
                     LFSLVALLLAAIGIYGVLASAVLERRSEIGVRMALGADRTSVIRMILSHTLRLTAIGI
                     TIGLAGSLAAADAIASLLFGVTPTDTATFVIAVGVLLGAAVVAAVVPARRAARLDPIT
                     VLRAE"
     gene            complement(4042..>4455)
                     /locus_tag="AC16CY_12675"
     CDS             complement(4042..>4455)
                     /locus_tag="AC16CY_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR3354694.1"
                     /translation="FKGALFLNTLRSVVDDDAVWWTLLRDFYQQFKYRNILSEDVVRF
                     FNTRLERDLTPLFDQYLRRADLPTLELAFGEPGTLSYRWKAEERAFSMPVRVGSPGNW
                     TLLQPTTEWKSMKTTLQKDALEVATDLYYINVVKQ"
BASE COUNT          673 a         1437 c         1526 g          819 t
ORIGIN      
        1 cgaccgccct cgtgttcgac tcctcggtcg agggcgtgaa gcacaaagcg cacacgaccg
       61 gcgagcttca cgatgccgtg aggagcctgg gcgcgtcgcc gctcaactgc gtctacatca
      121 cgcaggatcg agggttcgag cgcgagtacc gcgagcactg ttcgtcaatc ggtttcgatc
      181 ccgtgctcgc catcctgaac cacgactact gggtctggta cgcctttgcg cagtacgaga
      241 ccgacggcga gcgcatcttc gagcagcggc tcgaagcctt ccggtcgcgg cccgctcaac
      301 gcgcccgccg gtttctgtcg ctcaatcgca cggcgagaga ggcgaagctc atcttcctgc
      361 tcagcctgct ccgcgacgga ctctgggaca agggcttcgt ctccttcggc ggattccagc
      421 ggcccgatca gccgaagggg gagaagccga gaccgacacc cgagcagctt gccaggtcga
      481 tcccgggttt cgacgacaag gtggccgagc tcgcgccgtt tctcgatgcc ctggatgcgc
      541 gcggacgcgt actgctcggc atggagcgcc acggatggac gcggctcgaa ctgtggaacg
      601 caggcctggc ggccgatctg gccgagtacg accagtcctg gttcagcgtc gccaccgaga
      661 ccgagatgcg gccacggctc tcgcgcatca ccgagaagtc ggtcaagccg ctcgccaact
      721 tccatccgat gctcgtgctc ggcaacccgg gatcgctgca gatggtcaga agctacgggt
      781 tcgtgacctt cgaggagatc ttcgacgagt cctacgacga ggagctcgat ccgcgcaggc
      841 gcttcgacat ggtctacggc gagctgaccc ggttgtgccg gctggacgag aaggagctgt
      901 tcgcgatgga gcggcgcgtg gaggacaagc tgatcttcaa cgccaggtgg gggttcaccg
      961 agtttcccgg agcctaccgg cgccagcgcg acacggcgct ggtcgatgag atcctggccg
     1021 cagtcagccg gaccccatta ccgcccgctc acggtacttg accgggagtc gggcggcgac
     1081 cacaataggc atagttgacc tatgcctagg gacgctggac tcccgccggg gacgctcatg
     1141 atgctggtgc tgcgcgttct gacgacgcag ccgctgcacg ggtacgcgat cgcgcagcgc
     1201 ctccatcagc tgtcgcgcga ggagctcagc gtcgaggaag gctcgctgta ccctgcgctc
     1261 cagaagctgc tcgtcaaagg ctgggtgaga gccgcgtggc gcgaatcgga gtccgggcgt
     1321 caggtgcggg aataccgcat caccgccagc ggacgccggc agctcgacgc cgagatggcg
     1381 gagtaccggc ggacggcgag tgcgattggc tggctgctgg atgaggcgtg aggttcatgg
     1441 gcatcatcgg tgagtggatc cgtcgtctcg gctacctgct ccgacgaggc gcgcgagagg
     1501 aggagctgcg ccgggagatg gaggcacacc gcgcgatgct gcaggagcca agggcgttcg
     1561 ggaacacgct gcgcctgcgc gaggaggcgc gggatgcgtg ggggtggggg tggctcgacg
     1621 acctcgcgcg tgacacgcgc ctcgcctggc ggacgttgcg ccgcagtccc gcgttcgcgc
     1681 tcaccgcgat cgtgacgctt gcgctcggca tcggagcgac gggcgccatc ttcacgctcg
     1741 ttcgcagcgt gctgctgcgg ccgctgccgt tcccggatcc tgacgcgttg tacgtgattt
     1801 cgtacgcctc cccaggaccc gcctggctct accccggtat gtcggatcgc ggttatctcg
     1861 attttcgtgg agcgaaccgc accctcgagt cagtcgcgac tttctcagcc gccgagtcca
     1921 cgatgaccgg cgcgggaaat gcgactcggg tgtccggcgc agcggtcacg accgatttct
     1981 tccgtgtcct cggcgtcgac gccgcgatcg gacggacgtt tggaccggaa gacgatcaat
     2041 cgggagacgg cagggtcgtc ctgatcgccg acggcctgtg gcgacgccat ttcggtgccg
     2101 atcccttgtt cgtcaacaag accgtcgtgc tcaatggcgt gccgcacaga gtgatcggcg
     2161 tgcttcctcc gggcttctcg tatccggcgg acgcggcgta ctggattccg ctgacagttc
     2221 gtatcaatcc aaacctcggc ttcatgcgtc ccgtgatcgg ccgcgtgaag gccggcgtca
     2281 caggcgaaca ggccgcagcg gacctcgacg tgtgggtacg gagtctgccg ctcgatccga
     2341 accggcctcg cgatctcgtg gcgcaggtga cgccgctgca cgaggccatg gtgcgggatg
     2401 ttcgattgcc ggtgctggtc tttggcagtg cggtggtcct ggtcctggtg atcgtgtgcg
     2461 ccaacgtcat gcacctgctg ctgatgcgcg ctgtgtcgcg ccgtcaggag atcgcgacac
     2521 ggctggcatt gggtggaagc cgagttcgtg tcgtacgaca gttcctggcc gagggtgcgc
     2581 tgctgtcgct cgccggcggg agcgtgggcg cgggtgtggc gttcctcggc ggtccggcgc
     2641 tgctatcgct gattcccccg ggtcggctcc ccgtggacat tgcggttcgc gcggacggct
     2701 gggtgctcgc gtttacggca gggctgtcgg tgtgcacggc tctcgtcgtg gggctcgcgc
     2761 ccatcgtgca gatatcgggc gacagtccgt acgttgcctt gcgcgggggg acggcatccg
     2821 ccacgcgtca gtcgcatcgg ctgcggcatg ccctcgtgat ctccgaactc gcgctgacgc
     2881 tcgtgctgct cgtgggcgca ggcctgctcg tcaggagcgt cattggactg cgcagtgttc
     2941 cgctcggatt cacaccgggg cgtgtgatga cgatgacgat cgatctcccg gtgacgaggt
     3001 acccgaaagc tgagcaggcg acgacgctgc actcgcgctt gctgcagtcg ctctcggcct
     3061 ctccgggcgt gctctccgtt gccgccgtga actggctccc gctcggcgac atggtgagct
     3121 ggggcgacgt gcaggccgag gatcgccccg atctcgccgg aaagtacaac gcgacgaagg
     3181 tcgccgtcag tcccgattac ttccgaacga tcggcatcag gctgctgcgc ggccgcccgt
     3241 tcacggaagc cgacctcccc ggccggccac ctgtggcgat cgtcagtgaa tcggttgcgc
     3301 gacgcctgtg gcccggcgtc gatccgtcgg gcaggcgaat ggcgctcgtg gatcggccga
     3361 agcccgagga ctggctgacc gtggtcggtg tcgtcgagga cgtcaggcag ggcggattca
     3421 ggtcggctcc cacgcacgcg gtctaccagc cctacgtgca ggtcacgaac cggtatttcg
     3481 ttggttacat gacgtttctc gtccgcacgc acggggatcc ccttcaggtg gcgccgacga
     3541 tgcgaaccgc gttggctcgc atagacgagg acgaggcacc gcggtcgctt gcgacgctcg
     3601 aaacggtcgt cgatcgaacg gttgccgagc cacaatttca cgcgcgcgtg ctcacgctct
     3661 tttcgctcgt cgcgcttctg ctcgcagcca tcggcatcta cggcgtgctt gcgtcagccg
     3721 tgctcgagcg ccgatccgag atcggtgtcc gcatggcgct cggcgccgac cgcacctcgg
     3781 tcatccgcat gatcctgtcg cataccctgc ggctcaccgc gatcggcatc acgattggcc
     3841 tggcgggctc gctggccgcg gcagacgcca tcgcttcgct gctctttggc gtgacgccga
     3901 cggatacggc gaccttcgtc atcgcggtgg gcgtgctcct tggcgcggcc gttgtcgctg
     3961 ccgtcgtgcc ggcgcggcgc gcggcgcgcc tggatccgat caccgtcctg cgagcggagt
     4021 aggccagcgc tagttggcgc gtcactgttt gacgacgttg atgtagtaga ggtcggtggc
     4081 gacttcgaga gcgtccttct ggagagtcgt cttcatggac ttccactccg tggtcggctg
     4141 gagcagcgtc cagttgcctg gcgagccgac ccgcacgggc atcgagaacg cccgttcctc
     4201 ggccttccag cgatacgaga gcgttcccgg ctcgccgaac gcgagctcga gcgtcgggag
     4261 atccgcccgg cgaaggtact ggtcgaacag cggagtgaga tcgcgctcga gcctcgtgtt
     4321 gaagaaccgc acgacgtctt cgctcaggat gttccggtac ttgaactgct ggtagaagtc
     4381 gcgcagcagc gtccaccaca ccgcgtcgtc gtccaccacg ctcctcaacg tgttcaggaa
     4441 aagcgcgccc ttgaa
//
LOCUS       DBNGSH010000553         7421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_733606, whole genome shotgun sequence.
ACCESSION   DBNGSH010000553 DBNGSH010000000
VERSION     DBNGSH010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_733606"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="AC16CY_12680"
     CDS             <1..371
                     /locus_tag="AC16CY_12680"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3354695.1"
                     /translation="LFVRTGVWARRKAVGPWLRGRAEGGQSAGLHPSVIPWLKARDIA
                     LLGSDHPQYVSPGTPGAPRGAVHDFALVYLGVHLFDNCDLEALADAAAGRKRWEFLLT
                     AAPLPIRGGTGSPLNPIATF"
     gene            449..694
                     /locus_tag="AC16CY_12685"
     CDS             449..694
                     /locus_tag="AC16CY_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3354696.1"
                     /translation="MKRAISAADANREFSAVLRHVRRGHSFVVTSHGRPVARIVPFAA
                     GEHVTATARATLFKRLAAAPVVAAGGWTRDELYEDSR"
     gene            691..1149
                     /locus_tag="AC16CY_12690"
     CDS             691..1149
                     /locus_tag="AC16CY_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3354697.1"
                     /translation="MNVAVDTNVLAYAEGMNGAARKGTALHVLESLSPESTFIPVQVL
                     GELFAVLVRKAGWSRTKAGTAILGWGDVFPLIDSSNDVLLGATDLVSVHQLGFWDAVI
                     LSAAADARCRLLLSEDLQEGFTWRGVTVTNPFSRSRHPLLEAVLGASKAR"
     gene            complement(1206..1694)
                     /locus_tag="AC16CY_12695"
     CDS             complement(1206..1694)
                     /locus_tag="AC16CY_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354698.1"
                     /translation="MLGDRMLVLAVAALLTVNAVAAQGPVVPPPQSPPVQPTLKAEEH
                     PVVVSGCVRGSRLKRSGRVTDSAADALNASEFVLEGPRELMQQIRREHDGHYDELTGI
                     AILPPSRNDGSSLVGTKKIGPGQVSIGARREAGQQLEAPAPVRIRVSSFRHVDEACAG
                     RG"
     gene            1789..3963
                     /locus_tag="AC16CY_12700"
     CDS             1789..3963
                     /locus_tag="AC16CY_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3354699.1"
                     /translation="MTRNAVRLLVALVAVVVGVAALMGGVGAGQVATRPVAIDSDDIG
                     GIVTGPKGPEAGVWVVAETTQLPTRLIKSVVTDDQGRYVLPDLPKADYDVWVRGYGLV
                     DSPKVKATPGSQMPLKAVPAPNSRVAAEYYPAQYWLSLLQVPPKSDFPGTGPSGNGIS
                     SDVRSQGEWIRNIVNTDGCTGCHQMGGKATREIPEELGRFESSKAAWDRRIQSGQAGR
                     GMSQRFSQVGRERALAMWADWTDRIAGGELPATAPARPQGRERNVVVTMWDWADPKAY
                     LHDEIASDKRNPTVNANGPIYGALEASADYMPVVDPVRNTATQVKLVVRDPATPSEAK
                     TPPAQPSPYWGSEPIWTSQANAHSFAMDRQSRVWIAARIRPNQTASFCQKGSTHPSAA
                     AFPIAQSGRQMQMYDPKTKTVTTIDTCFGTHHLNFDDNDVLWFTGGGAVEGWFDTRIY
                     DKTKDETKAQGWTVFVLDTNGNGKRDEYVEPDQPVDPAKDKRVNAPFYGVAPSPVDGA
                     IWGSVLGMPGMLVRLTLGSNPPATALSEVYEVPWNNAKAPAQGFAPRGMDVDSNGVVW
                     TVLSSGQLASFDRRKCKGPLNGPNATGQHCAEGWALYALPGPNYKGAADSASADTAYY
                     NFVDRFDMLGIGKNVPLATGNGSEGLLALVDGKFLTFRVPYPMGFYSKGLDGRIDDVR
                     GGWKGKGIWTTYATRAPFHAEGGKGTTSKLVKFQVRSSPLDK"
     gene            complement(3993..5003)
                     /locus_tag="AC16CY_12705"
     CDS             complement(3993..5003)
                     /locus_tag="AC16CY_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354700.1"
                     /translation="MARRGFGSCFIAAGAAALTVLLSVPAISQTYTVPRTPWGDPDLQ
                     GIWPGNMGVPLQRPMDFGERQVLTEGEFAKREAQAAAQAKADAEIYARTGGTGIGGPS
                     HWVERGRPTRQASLVVDPPNGRLPALTPEAERRRKEARGGRGFTGEWRGEADSFEDLN
                     IYYRCITRGVLGSVIPVIYSNGNEIVQGPGYVVFRNEMIHESRVIPLDGRPHVGSALR
                     MYMGDSRGRWEGDTLVVETTNLTDKVAIGSNGAGYPGDPGYHSEALRLTERFTRTAEN
                     TLEYRVTVNDPQTWTKPWTLLIVLARSSDPHLHEYACHEGNYAMRNILSAARADERAR
                     QR"
     gene            5087..5554
                     /locus_tag="AC16CY_12710"
     CDS             5087..5554
                     /locus_tag="AC16CY_12710"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HIR3354701.1"
                     /translation="MANCANCGAPMHPDTPRGVFVCGHCGSVDEGPSVAAHLDIGGQS
                     STKCPACATPLADSRLDGNSVLFCRRCEGLLVAMACFVDVAEALRSREDRARVILPRE
                     QVPGARELSCPRCGGPMLNHVYGGPGNLVIDTCESCRLNWLDPGELRRLARAL"
     gene            complement(5690..6040)
                     /locus_tag="AC16CY_12715"
     CDS             complement(5690..6040)
                     /locus_tag="AC16CY_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR3354702.1"
                     /translation="MKLLFDENVSPKLTTRLASEYPGSAHVRDVGLRGAEDRQVWDYA
                     RTHGFVIASKDTDFRERSYVEGAPPRVIWLDVGNAGTDAIAQLLRAQVERARQFDSLE
                     GASLLILSISTAAV"
     gene            complement(6037..6261)
                     /locus_tag="AC16CY_12720"
     CDS             complement(6037..6261)
                     /locus_tag="AC16CY_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR3354703.1"
                     /translation="MSWQDRISVNPAIRSGKPCINGTRITVYDVLEYLAGGMTEEQIL
                     GDFPDLTREDIRAVLAFAAARERRLSNSVA"
     gene            complement(6465..6992)
                     /locus_tag="AC16CY_12725"
     CDS             complement(6465..6992)
                     /locus_tag="AC16CY_12725"
                     /inference="COORDINATES: protein motif:HMM:NF025292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR3354704.1"
                     /translation="MVMRKSKRAAIFGLLWLAVIPSTSAQRGTSDSGLVGTWRLVSAE
                     ERTEDGRTIPLWGSAPAGRLMYDGRGRMSAQLMDPRRKPFASEDRLGGTVAEMRGAFE
                     GYDAYFGSYVVDANAGVVVHHVMGSLFPNLIGTDQRRFFGLSGNRLTLTTPPFLRGAR
                     RSTYVLVWEREGQSE"
     gene            complement(7240..>7421)
                     /locus_tag="AC16CY_12730"
     CDS             complement(7240..>7421)
                     /locus_tag="AC16CY_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3354705.1"
                     /translation="ASSMNIGAHLVDMSPLSTTGAMCIAGITDVSQVRPTYNKLLAWG
                     LSMTVIGAVGCYLFL"
BASE COUNT         1271 a         2419 c         2451 g         1280 t
ORIGIN      
        1 cgctgttcgt gcgtacgggc gtctgggcca ggcgcaaggc cgttggacca tggcttcgcg
       61 ggcgggccga aggcggccag tcggcgggcc tgcatccgtc ggtcattccg tggctcaagg
      121 ctcgcgacat cgccctgctc ggcagcgatc acccgcagta tgtgtccccg gggacgcctg
      181 gcgcaccgag gggcgcggtc catgacttcg cgctcgtgta tctcggcgtg cacttgttcg
      241 acaactgcga cctcgaagca ctggccgacg ccgcggccgg gcgtaagcga tgggagtttc
      301 tcctgaccgc ggcaccgctg ccgattcgcg gcggcaccgg gtcgccgctc aatccaatcg
      361 cgactttctg aggcgccgag ctcgcccttg cctttccggc gccagttctg cgatagtagg
      421 taatatcact acatcaaggg agacggctgt gaagagggcg atctctgccg ccgatgcgaa
      481 tcgcgagttt tccgccgtgc tccgtcacgt ccgccgcggg cacagtttcg tggtgacctc
      541 acacggtcgg cccgtcgccc gcatcgtccc cttcgcggca ggggagcacg tgacggcgac
      601 cgccagggcg acgctcttca agcggctggc cgctgctccc gttgtcgcgg ccggcgggtg
      661 gacgcgcgac gagctgtacg aggattcccg gtgaatgtcg cggtcgatac caacgtgctg
      721 gcctatgccg aggggatgaa cggcgcggcg cggaagggga ccgcgcttca tgtattggag
      781 agtctgtcgc ccgagtcaac gttcatcccg gtacaggtcc tgggcgaact gttcgccgtc
      841 ctggtacgca aggcaggatg gtcgcggacg aaggccggca cggccatcct cgggtggggc
      901 gacgtcttcc cgttgatcga ttcgtccaac gacgtgcttc ttggcgcgac ggacctcgtg
      961 agcgtgcatc agttgggctt ctgggacgct gtgattctct cggcagccgc agacgcacgc
     1021 tgccgcctgc ttctgtcgga agatcttcag gaagggttca cgtggcgggg cgtcacggtg
     1081 acgaacccgt tctcgcgttc gaggcatccc ctgctcgagg cggtgctcgg cgcttcgaag
     1141 gcgaggtagc gaaagctccg acacacggct tcggctcggc tgacaatagg gccggagact
     1201 gccgctcagc ctcgccccgc gcaggcctcg tcaacgtgcc ggaaggacga cactcggatg
     1261 cggacgggcg ccggtgcctc gagttgctgg cccgcctccc ggcgagcgcc gatcgaaacc
     1321 tgtccgggcc cgatcttctt ggtgccgacg aggctcgagc cgtcgtttcg cgaaggaggc
     1381 aggatcgcga tccccgtgag ctcgtcgtag tgcccgtcgt gctcccgtcg aatctgttgc
     1441 atcagctcgc ggggaccttc gagcacgaac tcggacgcat tcagcgcatc ggccgcgctg
     1501 tccgtcaccc ggccagagcg cttgagccgg ctgcctctca cgcagccgga cacgacgacg
     1561 gggtgctcct ctgccttgag cgtgggctgc accggcggtg actgaggcgg tgggacgacc
     1621 gggccctggg cggcgacggc gttgaccgtc agaagagcag cgaccgcgag aacgagcatg
     1681 cggtcgccca gcataaggcc agtatacgcc tccgataacg cgcgtataat ccggccgatc
     1741 ccgccgcccg ttatccgggc agctcacatc gggcaggagg ccgctctcat gacgcgaaac
     1801 gccgttcgtc tcctggttgc actcgtggcc gttgtcgtgg gtgttgcagc gttgatgggc
     1861 ggtgtcggcg cgggccaggt cgcgaccagg ccggtggcga tcgacagcga cgacatcggt
     1921 ggaatcgtga ccggtcccaa gggaccggag gccggcgtgt gggtggttgc cgaaacgacc
     1981 cagctgccaa cgcgtctcat caagagcgtg gtgaccgacg atcagggccg ctacgtgctg
     2041 ccggacctgc ccaaggcgga ctacgacgtc tgggttcgcg ggtacggcct ggtggactcg
     2101 ccgaaagtca aagccacgcc cgggagccag atgcccctca aggcggtgcc cgcgccgaac
     2161 tctcgcgtgg ccgccgagta ctatcccgcg cagtactggc tgtcgctgct gcaggtgccc
     2221 ccaaagagcg actttccagg caccgggccg tcgggcaacg gaatctcgtc ggacgtccgc
     2281 agccagggcg agtggatccg caacatcgtg aacaccgacg gatgcacggg ctgccatcag
     2341 atgggcggca aggctacgcg cgagatcccg gaagaactgg gccggttcga gtcatcgaag
     2401 gccgcctggg atcgtcgcat tcagtcgggg caggccggcc gcggcatgag ccagcgcttc
     2461 agtcaggtcg gccgcgagcg cgcactcgcg atgtgggccg actggacgga ccggatcgcg
     2521 ggcggcgaac tgcccgcgac ggctccggca cggccgcaag gccgcgagcg caacgtcgtc
     2581 gtgacgatgt gggactgggc cgatccgaag gcgtacctgc acgacgagat cgcgagcgac
     2641 aagcggaacc cgacggtgaa cgcgaacgga cccatctacg gggcgctcga ggcgagcgcc
     2701 gactacatgc cggtggtcga tcccgtgcgc aacaccgcga cccaggtgaa gctcgtcgtg
     2761 cgcgatcccg cgacgccgag cgaagccaag acgccgcccg cgcagccgtc gccgtactgg
     2821 ggttccgaac ccatctggac cagccaggcc aatgcgcaca gcttcgcgat ggatcggcag
     2881 tcgcgcgtct ggatcgccgc gcgcatccgt ccgaaccaga cggcgtcgtt ctgccagaag
     2941 ggctcgacgc acccctcggc cgcggcgttt ccgatcgccc agagcggccg gcaaatgcag
     3001 atgtacgacc cgaagaccaa gacggtcacg acgatcgaca cctgcttcgg gacgcatcac
     3061 ctcaacttcg acgacaacga cgtgttgtgg ttcacaggcg gcggcgcggt cgagggatgg
     3121 ttcgacacgc gcatctacga caagacgaaa gacgaaacaa aggcccaggg ctggaccgtc
     3181 ttcgtcctcg acaccaacgg caacggcaaa cgcgacgagt acgtcgagcc agaccagccg
     3241 gtcgatccgg cgaaggacaa gcgcgtcaac gcgccgttct acggtgttgc gccaagcccg
     3301 gtcgatggcg ccatctgggg atcggttctg ggcatgccgg ggatgctcgt tcgactgacg
     3361 cttggatcga acccgcctgc aacggcgctc tcagaggtct acgaagtccc gtggaacaac
     3421 gcaaaggcgc cggcgcaggg gtttgcaccg cgcggcatgg atgtcgacag caacggcgtg
     3481 gtctggaccg tgctgtcgag cggacagctc gcgagcttcg atcgccgcaa gtgcaagggg
     3541 ccgctcaacg ggccgaacgc gacgggacag cattgcgccg agggttgggc gctctacgct
     3601 ctgccgggac cgaactacaa gggcgccgcc gactcggcga gcgccgacac ggcgtactac
     3661 aacttcgtcg atcggttcga catgctcggg atcgggaaga acgtgccgct cgcaacgggc
     3721 aacggatccg agggcttgct cgcactcgtg gacggaaagt ttctgacctt ccgcgtgccg
     3781 tatccgatgg gcttttattc gaaaggcctc gacggccgca tcgacgacgt ccgcggcggc
     3841 tggaagggca aggggatctg gacgacctac gcgacgcgcg cgccgttcca tgccgaaggc
     3901 ggcaagggga cgacgagcaa gctggtgaag ttccaggtcc gcagcagtcc gctggataaa
     3961 taggttccgg gcttcgggct acaggtcccg ggctatcgct gtctggctcg ttcgtcggcg
     4021 cgggccgcgc tgaggatgtt gcgcatcgcg tagttgccct cgtgacacgc gtactcgtgc
     4081 agatgcgggt cgctgctgcg cgccagcacg atgagcagcg tccagggttt cgtccacgtc
     4141 tgcggatcgt tcaccgtaac ccgatactcc agcgtgttct cggctgtccg tgtgaagcgc
     4201 tcggtcaggc gcagggcctc gctgtggtag ccgggatcac cgggatagcc ggcgccgttc
     4261 gatccgatcg cgaccttgtc ggtgaggttg gtcgtctcca ccaccagcgt gtcgccctcc
     4321 catcgtccgc gtgagtcgcc catgtacatg cgaagcgcgg aaccaacgtg cgggcgtccg
     4381 tcaagcggga tgacgcggct ctcgtggatc atctcgttcc tgaaaacaac gtatccgggg
     4441 ccctgtacga tctcgttccc gttgctgtag atcaccggga tcaccgagcc aaggacgccc
     4501 ctggtgatgc agcggtagta gatgttcagg tcctcgaacg agtcggcttc gccgcgccac
     4561 tccccggtaa agccgcggcc gccgcgggct tccttgcgac ggcgttcggc ctcgggcgtc
     4621 aaggccggca gccgcccgtt cggaggatcg acgacgagcg acgcctggcg ggtcggccgt
     4681 ccgcgctcga cccagtgcga gggtccaccg atgcccgtgc ccccggttct tgcgtagatc
     4741 tcggcatcgg ccttcgcctg tgccgcggcc tgtgcctcgc gcttggcaaa ctctccctcg
     4801 gtcaggacct gtcgctcacc gaagtccatt gggcgctgca agggcacgcc catgttgcct
     4861 ggccagatgc cctgaagatc cgggtctccc catggtgttc ggggcaccgt gtaggtctga
     4921 ctgatggctg gaacgctgag gagcaccgtg agagccgctg cccctgcagc gatgaaacag
     4981 gatccgaagc ctcgacgcgc catgtgagcc tccgctgtga tccgcttgaa tccgcgcgac
     5041 gggaccaagg gtaactcagg tcaggggtct ggccgataag gctttcgtgg ccaactgcgc
     5101 gaattgcggc gcaccgatgc acccggacac tcccaggggc gtgttcgtgt gcggccactg
     5161 cggatcggtt gacgaagggc cgtcggtggc ggcacatctc gacatcggcg gtcagagctc
     5221 gacgaaatgc cctgcctgtg ccacgccgct ggccgacagc cgtctcgacg ggaacagcgt
     5281 gctgttctgc cgccgatgtg agggcctcct ggtcgccatg gcgtgcttcg tcgatgtcgc
     5341 cgaggcgctg cgctcgcgtg aagatcgcgc gcgagtgatt ctgcctcgag agcaggtgcc
     5401 cggagcgcgc gagctgtcct gcccgcggtg cggcgggccc atgttgaatc acgtctatgg
     5461 cgggccaggc aatctcgtga tcgacacctg cgaaagctgc cgtctgaact ggctcgaccc
     5521 cggtgagctg cgccgcctcg cgcgggcgct gtgatgtacg gttggctatc cgcgaaggcg
     5581 gtcttctcga cgtgcagaaa gccgcatgac gaattagggc tgacccgcgg cggtcgctca
     5641 ccgctctgaa gcagcgcgat cgatccgcgg ccgcctacga gtgcccctgt cagaccgccg
     5701 ccgtcgagat cgacagaatc agcaacgagg ccccttcaag tgagtcgaac tgccgagcgc
     5761 gttcgacttg tgcacgaagc aactgtgcga tcgcgtcggt tcccgcattt cctacatcga
     5821 gccagatgac cctcggcggc gcaccctcaa cgtagcttcg ctcgcggaaa tccgtatcct
     5881 tcgacgcaat cacgaagccg tgcgtgcgag cgtagtccca cacctgccgg tcctcggcac
     5941 cacgcaaccc gacatctctg acgtgagcgc taccgggata ctcgctcgcg aggcgggtgg
     6001 tcagcttggg actgacgttc tcatcgaaga gcagcttcac gccactgagt tcgacagacg
     6061 tcgctcacgc gcagccgcga aggccaaaac ggctcgaatg tcctcccgag tgaggtcggg
     6121 aaagtccccg aggatctgtt cctccgtcat gccgccggca aggtattcca gcacgtcgta
     6181 taccgtgatt cgtgttccgt tgatacacgg cttgccgctg cgtatggcgg ggttcaccga
     6241 gatccgatcc tgccagctca tatccgggat tcgacctctg gcgccagacg ctctgagacc
     6301 gatctttcac gggcgtcacg aaatgcggtc aacgtctgcc gctcagccgc gagcggcgtg
     6361 gacgaggttc gagttacttc agcgtccccg caccactcgg cgactgcagc ggctgttcag
     6421 tggctccatt ccgatcacga tggtcgtagt cgtaatctcc tggcctattc gctttgccct
     6481 tctcgctccc acacgagcac ataggtggac cgtcgagcac ctcggagaaa cggaggcgtc
     6541 gtcagcgtca ggcggtttcc ggacagtccg aagaagcgac gctggtcagt cccgatcaga
     6601 tttgggaaca gagatcccat tacgtggtgg actacgacac cagcgttcgc atccactaca
     6661 taggaaccga agtatgcgtc atagccttcg aacgcccctc tcatctccgc tacagtcccg
     6721 cccagacgat cctcagacgc aaatggcttc cgtctagggt ccatgagttg ggcggacatt
     6781 cttccgcgac cgtcgtacat gagtcggccc gcgggagcgc tgccccagag cggtatcgtt
     6841 ctgccgtcct ctgtccgttc ttcagctgat acgagtcgcc atgtacccac aagccccgag
     6901 tcgctcgtgc cgcgctgtgc ggacgtgctg gggataacgg caagccagag aagaccaaag
     6961 atcgcggcgc gcttgctctt tcgcatcacc attcatgtcc gccgaacagt ctgtggagta
     7021 gagcgaacgg atcgctcgcg ggggtacaac gtcccaaact caccttaccg gtcacggcta
     7081 tctccaacga acgccggatg ataacacgca cgcgtggctc gtccccctcg ggtttagccc
     7141 gcaggccgag cggatgcgcg cggcccccgc gagtatcacg acggccacat ggtgatcagc
     7201 ctctcaaggg gctggcgtta tggccggcgt tcgcccggcc tacaggaaca gataacaacc
     7261 tacggcgccg atgacggtca tcgacaagcc ccacgcgagg agcttgttgt aggtcggccg
     7321 cacctgagag acgtccgtga tccccgcgat gcacatggcg ccggtcgtgg acagcggcga
     7381 catgtccacg aggtgcgccc cgatgttcat cgacgaggcg a
//
LOCUS       DBNGSH010000554         2943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_393870, whole genome shotgun sequence.
ACCESSION   DBNGSH010000554 DBNGSH010000000
VERSION     DBNGSH010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_393870"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC16CY_12735"
     CDS             complement(<1..518)
                     /locus_tag="AC16CY_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354706.1"
                     /translation="MRIPILRPVVACAVTVAFVSFAFAQRGPQQPPMVKEGVTEKISE
                     HVYVIPDGNVGMVPNVSIIVGTTGTFVVDTGLGARNGQAVMREVDKVSKNADLYLAST
                     HFHPEHDLGANGFPPRTKMIRSRDQLAELQEGGLDMAKRFASFSPAIAELLEGADFRK
                     ADILFDKEHVVDL"
     gene            607..1533
                     /locus_tag="AC16CY_12740"
     CDS             607..1533
                     /locus_tag="AC16CY_12740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3354707.1"
                     /translation="MRTRRLPDAAVRRLYGRARAEHWDVPFETFAAALERSAQHAASG
                     DLDAHALDAFLESLHLEDLALACACAAGHEGAWEHFIRQYRPVLYRAADAIDPSGDAR
                     EAADSMYGELFGLSEREGERRSLFHYFHGRSSMATWLRAVLAQRYVDRVRSVRRFEPL
                     PGDEALDRARAMAPGAPPPPAPGENAARQGYVAAIRRAVTAVVASLAPRDRLRLACYY
                     AQDLTLAQIGRALGEHEATVSRHLARARREIRHGVERHLREHEHMSEEAVAECFHSVV
                     EDAGSLDISEMLATSKESGNPRSKERRERANA"
     gene            1526..2536
                     /locus_tag="AC16CY_12745"
     CDS             1526..2536
                     /locus_tag="AC16CY_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3354708.1"
                     /translation="MREDDRDRAVDRLLRDSLKAQALTPAPKACVDGETLAAWTSGSL
                     RAERARDVERHLSDCTRCQALLAMFLRSMPEEPDVHPFWWRARLRWFVPLATAATAAA
                     IWLAIPVASPTRPARDQAQQLERPEAAATAALDQAADQEPPARLAKQEPPARPPEAPS
                     GTVAPADVGNRVAEERQETFSRESRLESSTRALGRQARADAGKEAAAVAQPRSVTGLE
                     ATEIEIPGPGVRWRIRAGRVVERSTSGGAAWDGVVIPVGPDALTAGSAVSSTVCWLVG
                     RGGTVYITADGVQFVRVAFPEAADLVAVRAIDERSATVTAADGRSWRTTDRGQSWTGV
                     GR"
     gene            2673..>2943
                     /locus_tag="AC16CY_12750"
     CDS             2673..>2943
                     /locus_tag="AC16CY_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3354709.1"
                     /translation="MTQVSITVNGKARKAHVEPRTLLVHFLREQLNLTGTHIGCDTTQ
                     CGACTVLIDGRSAKSCTIFAVQVDGAEVTTIEGLAKDGRLHPMQTG"
BASE COUNT          478 a          979 c         1005 g          481 t
ORIGIN      
        1 aggtccacga cgtgctcctt gtcgaacagg atgtcggcct tccggaagtc ggcgccctcg
       61 agcagttccg cgatcgccgg cgagaagctc gcgaagcgtt ttgccatgtc caggccgcct
      121 tcctgcagct cggccagctg atcgcgcgat cggatcatct tcgtgcgcgg cgggaacccg
      181 ttcgcgccga ggtcgtgctc ggggtgaaag tgcgtcgacg ccaggtagag gtcggcgttc
      241 ttgctcacct tgtccacttc gcgcatgacg gcttgaccgt tgcgcgcacc caggcccgtg
      301 tcgacgacga acgtgccggt ggttcccacg atgatcgaca cgttgggcac catcccgaca
      361 ttaccgtccg ggatgacgta gacgtgctcg gagatcttct ccgtcactcc ctccttgacc
      421 atcggcggct gctggggccc gcgctgggcg aaggcgaaag agacgaacgc gacggtcacg
      481 gcacaggcaa cgacaggacg caggatgggg attcgcatgt agcctctcgg gtatgaagtg
      541 tacgtcgtca tataccacac cgaaagtcat acagccgcaa gggaggcgtg agcccataat
      601 gtggccatgc ggacccggcg gctcccggac gctgccgtcc ggcgcctgta cggtcgagcg
      661 cgcgcggagc actgggacgt gccattcgaa acgtttgccg ctgccctcga gcggagtgcg
      721 cagcacgccg cgtccggcga tctcgacgcg cacgcgctcg acgcgtttct cgaatctctg
      781 cacctggagg atctggcgct ggcgtgtgcc tgcgccgcag gccatgaagg tgcgtgggag
      841 cacttcatcc ggcagtaccg gcctgtgctg taccgcgcgg cggacgccat cgacccgtca
      901 ggcgatgcgc gcgaggcggc cgactcgatg tatggcgagc tcttcgggtt gagtgagcgc
      961 gagggcgagc ggcgatcgct gttccactac tttcacggcc gcagcagcat ggcgacatgg
     1021 cttcgagccg tccttgccca gcggtatgtg gaccgcgttc gcagcgtgcg gcgcttcgag
     1081 cctcttcctg gggacgaggc cctcgacaga gctcgggcca tggcgcctgg cgcgcctccg
     1141 ccgcccgcgc caggcgagaa tgcggctcga cagggttacg tggcggcgat ccgtcgagcc
     1201 gtcacagccg tcgtcgcgag cctggccccg cgggatcgac tccgcctcgc ctgctactat
     1261 gcccaggacc tcacgctggc ccagatcggg cgggccctcg gcgaacacga ggccacagtc
     1321 tcacgccacc tggcgcgcgc ccgccgcgag atcaggcacg gggtcgaacg ccaccttcgc
     1381 gagcacgagc acatgagcga ggaggccgtt gccgaatgtt tccattcggt cgtcgaggat
     1441 gccggatccc tcgatatctc ggagatgctg gccacgagca aggaatccgg caatccccgt
     1501 tctaaggaga gacgcgagag agccaatgcg tgaagacgac cgcgatcggg cggtggatcg
     1561 ccttcttcgt gactcgctga aggcgcaggc cctcacgccg gcgccaaagg cctgcgtgga
     1621 cggcgaaacg cttgcggcgt ggacgtctgg ctcgcttcgg gctgagcgcg cgcgcgacgt
     1681 cgagcgtcat ctctctgact gcacacggtg tcaggccttg ctcgccatgt tccttcgatc
     1741 gatgccggaa gagcccgatg tccacccgtt ctggtggagg gccaggcttc ggtggttcgt
     1801 tccgctggcc actgcggcga cggcggccgc gatctggttg gcgatccccg tcgcctcgcc
     1861 aacccggccc gcgcgcgatc aggcgcagca actcgagcgc cctgaagcgg ccgcaaccgc
     1921 cgcgctcgat caagctgccg atcaggaacc cccggcccgg cttgccaaac aggagcctcc
     1981 ggctcggcct cccgaggcgc cgagcggaac agtcgcgcca gccgacgtcg gcaaccgcgt
     2041 tgccgaagag aggcaggaga cattcagccg agagagccgg ttggagagca gcactcgcgc
     2101 tcttggccgt caggcgaggg ccgacgcggg caaggaggcg gcggcggtcg cccaaccacg
     2161 gtccgtgaca ggcttggagg cgaccgagat cgagatcccc gggccgggcg tgcgctggcg
     2221 aattcgcgcc ggccgtgtgg tcgagcggtc cacgtctggc ggcgccgcct gggacggggt
     2281 cgtcattcca gttggaccgg atgcgctgac cgccgggtcc gcggtgtcgt ccaccgtctg
     2341 ctggctcgtc ggccggggcg gcaccgtcta catcacggcc gacggcgtcc aattcgtgcg
     2401 ggttgccttc cctgaagcgg cggatctcgt cgccgtgcgc gccatcgacg agcgcagtgc
     2461 gactgtgacg gcggccgacg gccggtcctg gcgcaccaca gatcgaggcc agagctggac
     2521 gggcgttggc cggtgaagcc ttcgaactac caactcccaa ttcccaactc ccaatgtctg
     2581 acttgtcgct tacgtcgatc ccacgcccct gggatttgga agttggaggt tgggagtttc
     2641 ctgtgtaggc tatgtggccg acgaggacac caatgaccca ggtttcgatc acggtgaacg
     2701 gcaaagcgcg caaggcccac gtcgagccgc ggacgctgct cgttcacttc ctgcgagaac
     2761 agctcaacct gacagggacg cacatcggct gcgacacgac gcagtgcggc gcctgtacgg
     2821 ttctcatcga cggccgcagc gcgaagtcct gcacgatctt cgcggtgcag gtggacggcg
     2881 ccgaggtgac gaccatcgaa ggcctggcca aggacggccg cctgcatccg atgcagacgg
     2941 gct
//
LOCUS       DBNGSH010000555        13546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3959608, whole genome shotgun sequence.
ACCESSION   DBNGSH010000555 DBNGSH010000000
VERSION     DBNGSH010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3959608"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..908
                     /locus_tag="AC16CY_12755"
     CDS             342..908
                     /locus_tag="AC16CY_12755"
                     /inference="COORDINATES: protein motif:HMM:NF033662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354710.1"
                     /translation="MTLNKIVATTSLLVACALFVVACTSQTSSLNPLGPTGVGSPALS
                     DVPSTESCTDGQDNDGDKLIDCADPDCAGDPSCGEGSKEVCTDGQDNDGDKLTDCADP
                     DCAEDPSCKTPPPPEGVPCSPGYWKNHESDFNEWCDEAAAIAGDPFTSCAQIWTAITC
                     SGGPRTGCTGERRQAAASALNAVSGCTE"
     gene            1004..1996
                     /locus_tag="AC16CY_12760"
     CDS             1004..1996
                     /locus_tag="AC16CY_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HIR3354711.1"
                     /translation="MTKYAFRVMLIAGSMVFLAAHATSAQVTEMLPNLSPFDAVDIRF
                     DSNGSATLQFSTISRNLGAGPMEIVGGPTDPVAQKQVVYQRIYLTDGTFYDRVAGTFE
                     YHDGHFHFHLNDYAKYVLRPLGPSGSGDVESQKTSFCLLDNQVVNLTLPGAPQQPVYT
                     TCNPDRQGISVGWGDKYGYQLPGQSFDVSGKPDGDYQLIIEIDPLNHLRETNNSDNTS
                     CVVVRLSVAARTVSNRGPCTSTNVTVSSITPNNITSGSIINVVIKGSGFATGMAVGFE
                     NGSGPAPVASAVQVVDGNTITARVSVKNGGPRRSRVWDVRVGPAVLSRGFTVTP"
     gene            2104..3018
                     /gene="cysD"
                     /locus_tag="AC16CY_12765"
     CDS             2104..3018
                     /gene="cysD"
                     /locus_tag="AC16CY_12765"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR3354712.1"
                     /translation="MNSLHARLTHLQRLEAEAIHIMREVVAECERPVMLYSIGKDSSV
                     MLHIALKAFHPAPPPFPFLHVDTTWKFREMIAFRDEAARRHGLDLIVHINQEGLASGI
                     NPFTHGSNVHTDVMKTQALRQALDRYGFDAAFGGARRDEEKSRAKERVFSIRSAQHRW
                     DPKRQRPELWRVYNTRTRDGESLRVFPLSNWTELDVWQYIYVEQIPIVPLYFARERPV
                     VRRAGTWIMVDDNRMPLEPGETPQSKMVRFRTLGCYPLTGAIESDASSLPDILREMLA
                     TTMSERQGRVIDHDQSASMEKKKVEGYF"
     gene            3030..4925
                     /gene="cysN"
                     /locus_tag="AC16CY_12770"
     CDS             3030..4925
                     /gene="cysN"
                     /locus_tag="AC16CY_12770"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HIR3354713.1"
                     /translation="MEAFDADLEGYLRSHERKDLLRFITCGSVDDGKSTLIGRLLYES
                     KMIFEDQLAALAADSRTMGTRGGELDFALLVDGLSAEREQGITIDVAYRFFATEKRKF
                     IVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSHVVALLGIRRVVLAV
                     NKIDLVDYSSEVFERIERDYREFARRIGLDDVTCIPISAIHGDNVVRRADATRWYRGP
                     TLLEHLETVPVGEDLAARPFRMLVQWVNRPSQDFRGFAGVVVSGGVRVGEAVRILPSG
                     RPSTVARVLVGDTDAQAAVAGQSITLTLTDEIDISRGDVLAAPDAAPPVADQFEATVV
                     WMHEQPLLQGRSYLMKIGTKTVPATVSPIKYKLNVDTLEHVAARTLELNDIGVCALEL
                     AQPVVFEPYADSRDLGGFILIDRVSNATVGAGLLRFALRRSQNVHWQALDVDKVARAG
                     LKGQKPCVIWFTGLSGAGKSTIANLVEKRLLALGRHTYLLDGDNVRHGLNKDLGFTDA
                     DRVENIRRVAEVARLMVDAGLIVLVSFISPFRSERRMARELLGEGEFLEVFVDTPLDV
                     AEGRDPKGLYKKARRGELRNFTGVDSPYEMPEHPDVRVDTTRLSAEDAAGVILERLGR
                     TGVIDVE"
     gene            complement(4946..6847)
                     /locus_tag="AC16CY_12775"
     CDS             complement(4946..6847)
                     /locus_tag="AC16CY_12775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR3354714.1"
                     /translation="MRYRPEVDGLRAVAVLPVILYHAGLSVARGGFVGVDVFFVISGY
                     LISSIIIDQHQRGRFSLLDFYARRARRILPALFFVILCTMPFAWLWMVPGQLRDSSQS
                     VAAVSVFASNFLFWFHSGYFDPLAAERPLLHTWSLGVEEQFYVLFPVTFLLFWRFGIR
                     WLSALVVTVTIASLALAEYWQPLDASAAFYLLPTRAWELSIGVLCALSQLKQGQLKNE
                     ALSALGLALIVTSVVLYDETVPFPSLFALVPVGGAALVILFAAGQTGTARLLSAKPLV
                     VIGLISYSAYLWHHPLFALARIRSFEPPGTFSMLALGGLSLLLAYVSWRFVEQPIRLF
                     RGWSAPTVLAAAAGVSVLFFSVGTSGYLTDGFRSVKTTPEQRALLDMAQNSPKRPECH
                     TRGRDYLKPADACEYFDGAVRWAVLGDSHAVELALAVAEQGRQVGVTVKHLSFSACGP
                     VLDHTAADADCRNWTREAVAFLAGNSGIGTVIVNYRLHLYLYGDHAGVFPRLPNTVEE
                     NVRNAYWKDLVRILGRLTDAHKKVVLVLQAPELRRHVEELIMEPGSRGDRIGVTRLWW
                     DQRKAFVDTHLSELPRDVLVVDPTDLFCDRDNCYAVRDGQTLYYDRDHLSVTGASFVA
                     RRLFEILLP"
     gene            7319..8341
                     /locus_tag="AC16CY_12780"
     CDS             7319..8341
                     /locus_tag="AC16CY_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354715.1"
                     /translation="MDRLARWVEVEQDGYLWWKEEWMQLFGAENFVDRGGDRILLAGS
                     SEVREGFLFDEFEAELQGFEVYNNAFSNQTLETLVIVLQYIETVYGPSAMPRKIVVGF
                     TPQTLLNVPTLEASYWPRVIDRYSPQVKLDVGSRPARLIRKGRREAVTARYRYFTHQA
                     RRYQGALRGVMRASILSVSPGMADRYWLRLGLVPSLYHHLPRRDQREQLQAFRRVLPS
                     PPDPVAMAATVRAQWAVLRDLMAAHDVELYVVNVPQSSLLRDDYYSAVYDDYEQLLRS
                     VVGNTPYLDLARYLRDDEFFDLTHPTLAAARRTSRRVAQFVREADTGRLPYQAARRSV
                     NVSGRS"
     gene            8381..10135
                     /locus_tag="AC16CY_12785"
     CDS             8381..10135
                     /locus_tag="AC16CY_12785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3354716.1"
                     /translation="MPPAVTESRHLFRYARPFLVLISIATILSVGARGLVLVLPQLAS
                     RIIDTAQGQLPPSLLSTTGALLLGALLLAALLQFAGAVMFSYVGERVVKNLRYDLFSH
                     LLSLSMSFFEQRRVGEMISRVAADATTVREIATSLPQLVVNHGVIAVGAVAIILVRHP
                     TLTLIMLAFMPFIALSGAIAGRRVRRLAVRSQDDLAQTNVGLEEALSGIATVKAFAQE
                     ETETSRYTTRLSALFRSSMRVFVAQESMRLTSLLIVYGGLVTVVWYATTQILAGALTT
                     GELVAFMMYALFAGNSFRSLVDVWSRFERLRGITLRLVQIFHERPSVVDRPDAVGFDG
                     SHRRIAFHDVSFSYPTDPGRQVLVDLDFSFERGEHIALVGESGAGKSTVAALLLRLYD
                     VTGGSITIDGRDIRDLSQKDLRRAIAVVPQDIVVFGRSVRENIGYGRRDATDEQIMAA
                     ARAAKALEFIERLPQGFDTVLGVRGVTLSGGQRQRIAVARAIVKDPAILILDEATSAL
                     DKPSEALVREALANLMKGRTTIVIAHRLATIEGADRVLVLHEGRLVESGTPHDLLRAG
                     GRYAWLHNSGVWEGGPAA"
     gene            10132..12201
                     /locus_tag="AC16CY_12790"
     CDS             10132..12201
                     /locus_tag="AC16CY_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3354717.1"
                     /translation="MSTLVDLVGRRAAVDPDRTAYRYLDDGEGNERRFSYRELDASAR
                     AIAGRLQEQGVRQGDRVLLLYPSGLDYIAAFFGCLFAGAVAVPAYAPRPKGPDGRLDA
                     IVKDAGAAVALTTEEVLSVIRQLPAGDFHREVKTWIATDHVDANDAGLWRLPNLDDDT
                     LAFLQYTSGSTSTPKGVMVSHGNLMANERMLQVALEQDQDSTWVGWLPMFHDMGLIGN
                     VIQPLYVGAACVLMSPMAFVQEPIRWLRAITKYRAHTSGAPNFAYDLCVAKTTPEERE
                     GLDLSSWKLAFNGSEPIRAETVDRFSHAFEPFGFRRSAFYPCYGLAEATLFVSGPVKS
                     SVPEVRTFEAAGLEQRQAISTPEGDEGGRTLVGCGRTWLDQTIVIADPDTSNRCPDGR
                     IGEIWISGPNVTKGYWGREEATTATFRARLAHGGEGPFLRTGDLGFFHGGSLFISGRI
                     KDLIIIRGRNHYPQDIELTVESSHPILRPGCGAAFAIDVDGDERLVVVQEVKRNFKDA
                     NLHDVVDVIRRAVSENHELAAHAVVLIKPGRIPKTSSGKIQRHACRQGYLAGALDPLL
                     SSAADAAAEGATPVRPAAASLLQQTLAVLGPAGSRPVMESHLRHRVSEVAAANGAGLD
                     AMNAEARLRLGRVLETDLGVSVPDALVFEATSIEALAANLIDLVIGRGESGESGARPD
                     PTRRVVA"
     gene            12237..13301
                     /locus_tag="AC16CY_12795"
     CDS             12237..13301
                     /locus_tag="AC16CY_12795"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR3354718.1"
                     /translation="MNDSKRAATPEPPLRRRARPQAVAFSEMVLTKTRTLSPELTLPL
                     VIEPAIDNVDLVAWARDNSEYLDRELLKHGALLLRGFETNLDVFEAAAGAVSKELFRD
                     YGDLPQEQGKDAVYHSTPYPENEMIDFHNESSHLRSWPEKQFFMCVLVSEEGGVTPIA
                     DCREVYRLLSPELRDKFHSKGLKYVRNFIPKLDVSWQQFFRTENRAEVEAKCRETGTD
                     FTWKDGDRLKIESWAPGVITHPRTGELVFFNQVRLHHVSCLPAAYRESTLSMFAEDDL
                     PRNVVFGDGTPITDDEIAQVGLAFDKAVVDAPWQKGDIMMLNNMLVAHGRRPFKGKRK
                     IVVAMADMWKVEDVQSRPKA"
     gene            13344..>13546
                     /locus_tag="AC16CY_12800"
     CDS             13344..>13546
                     /locus_tag="AC16CY_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3354719.1"
                     /translation="MSLLKTAFAALDKVQREQLLEAHLRDRVARVLGVSVAEVDAQRP
                     LGSLGLDSLKAVELQHGLERELS"
BASE COUNT         2506 a         4226 c         4375 g         2439 t
ORIGIN      
        1 caacgtccag ccgagaccgt cttgaacgcc gtccccaggt cagcaaactc cgatcctacc
       61 gactcccgag cgaagcccca catgatcgat ggttgcacga ttgtactgcc ccttcttcgg
      121 agaccaaggt ctccctcaac cggtactttg ggtcggttgg ggattgacgc acgagcgtgg
      181 aggtttccag cgaacctctt tatcgccgca acttgcccgc aagtcttggt gaacaccacg
      241 gcgtggcagc cggattgctt tgtgagcgcg cccatgtatt ggcgacaagc tgatttttgt
      301 ctccccactt tccccccttc aaggttctac ggaggctaag catgactctc aacaagatcg
      361 tcgctacgac cagtctgctc gtagcgtgcg cactgtttgt cgttgcgtgt acgagccaga
      421 cgtcgtcgct gaatccgttg ggtcccacgg gtgtcggcag cccggcactc agcgacgttc
      481 cttcaacgga gtcctgcacc gacggacagg acaatgacgg cgacaagctg attgattgtg
      541 cggaccctga ttgcgctgga gatccgtcct gcggcgaggg ttcaaaggag gtctgcaccg
      601 acgggcagga caacgacggc gacaagctga ccgattgtgc ggaccctgat tgcgccgaag
      661 atccgtcctg caagacgccg ccaccccctg agggagttcc ctgctcgccc ggctactgga
      721 agaatcacga gagtgatttc aatgagtggt gcgacgaggc agcggcaatc gccggagatc
      781 cattcaccag ctgcgcccag atatggacgg ccatcacctg cagcggtggc ccaaggactg
      841 ggtgcaccgg tgaaaggcga caggctgccg ctagtgcgtt gaacgcggtt tctggttgca
      901 ccgagtgacg ggcaacacga agtgagtcag ctcttcccgg tcagtgccga ctggccggga
      961 agagttgttt ttccagtacc ggcttcgtgt ctgggacact gcaatgacga aatacgcttt
     1021 tcgtgtgatg ttgatcgcgg gctccatggt cttcctcgca gcgcacgcga cgtccgctca
     1081 agtcacggag atgctgccaa acctgtctcc gttcgacgcc gttgacatca gattcgactc
     1141 gaatggatcg gccacgttgc agttctcaac gatcagccgc aacctcggcg ccggcccgat
     1201 ggagatcgtt ggcggaccga ccgatcccgt cgcccagaag caggtcgtgt atcagcggat
     1261 atacctcacc gacggcacct tctacgatcg agtggcggga acgttcgagt atcacgacgg
     1321 acacttccac ttccacctga acgactacgc aaagtacgta ttgcggcctc tcggcccctc
     1381 gggaagtgga gacgtcgaga gtcagaagac cagtttttgc cttctcgaca accaggtggt
     1441 gaatctgacc ctgccgggcg ctccgcagca gcccgtctat acaacctgta atcccgacag
     1501 gcaagggatc tcggttggct ggggagataa gtacggctat cagctcccgg ggcagtcgtt
     1561 tgacgtgagc ggcaaaccgg atggtgatta ccagctcatc atcgaaatcg atccgctcaa
     1621 tcatcttcgc gagacgaata acagcgacaa cacgtcttgt gtggtcgtcc gattgagcgt
     1681 cgcggcacgc acggtcagca atcgcggtcc ctgcacgtcc acgaacgtaa ccgtgtcatc
     1741 gatcactccc aacaacatca cgtctggttc gatcatcaac gtggtgatca aaggcagcgg
     1801 ctttgccacg gggatggcgg tcggcttcga aaacggctcc ggtccagcgc ccgtcgcgag
     1861 cgctgtgcag gtcgtcgatg gcaataccat cacggcacgt gtgtcagtca agaacggtgg
     1921 gcctcgccgg tcgcgggttt gggacgtgcg ggtcggtcct gctgtcttgt cgcgaggttt
     1981 tactgtgacg ccctgactcg atgccggata agtgatgagc gcccgtttcg tctacgtgac
     2041 ggagcgggcg ttcgcatgta cagccacatg caaccgggag agcgaccgat atcattaaag
     2101 gcagtgaatt ccttacacgc gcggctgaca cacctgcagc ggctcgaggc cgaggccatc
     2161 cacatcatgc gcgaggtggt cgccgagtgc gagcgcccgg tcatgctgta ttcgatcggc
     2221 aaggacagct cggtgatgct gcacatcgcg ctgaaagcgt tccatcccgc cccgcccccc
     2281 tttccgtttc tgcacgtcga caccacctgg aaattccgcg agatgatcgc cttccgcgac
     2341 gaggccgccc gccggcacgg gctcgatctc atcgtccaca tcaatcagga ggggctcgca
     2401 tccggcatca atccgtttac gcacggatcc aacgttcaca ccgacgtcat gaagactcag
     2461 gccctcaggc aggcgctgga ccggtacggc ttcgacgccg ccttcggcgg cgcgcgccgg
     2521 gacgaggaga agtcgcgggc caaggagcgc gtgttctcga tccggtcagc ccagcaccgg
     2581 tgggacccga aacggcaacg gccggagctc tggcgggtgt acaacacccg cacgcgcgac
     2641 ggcgaaagcc tccgtgtctt tccgctgtcg aactggaccg agctcgacgt ctggcagtac
     2701 atctacgtcg agcagattcc catcgtcccg ttgtactttg ccagggaacg gccggtcgtg
     2761 cgccgggcag gcacctggat catggttgac gacaaccgga tgccgctcga gccaggcgaa
     2821 accccccagt ccaagatggt ccgcttccgg acgctgggat gctatccgct gactggcgcc
     2881 atcgaaagcg acgcgtcgtc gctgcccgac atcctccgag agatgctggc gacgacgatg
     2941 tcggagcgtc agggacgggt catcgatcac gatcagtcgg cgtctatgga aaagaagaag
     3001 gtcgagggat acttttgacc ttcagggtca tggaggcttt cgacgccgat ctcgaagggt
     3061 acctgcgctc gcacgagcgc aaggacctgc tgcgcttcat cacgtgcggc agcgtggacg
     3121 acgggaagtc gacgctgatt gggcggctgt tgtacgagtc gaagatgatc ttcgaggatc
     3181 agctcgcggc tctggcggcc gactcgcgga cgatggggac tcgcggcggc gagctggact
     3241 tcgcgctgct ggtcgacggg ttgtctgccg agcgcgagca gggcatcacg atcgacgtcg
     3301 cgtatcgttt ttttgccacc gagaagcgga agttcatcgt ggccgacacg ccgggtcacg
     3361 agcagtacac gcggaacatg gtgacggggg catccacggc ggacctcgcc gtcatcctca
     3421 tcgacgcgcg gaagggcgtc ctgacccaga cacgccggca cagccacgtc gtggcgctgc
     3481 tcggcatccg ccgcgtggtg ctggccgtca acaagatcga cctcgtcgac tattcgtcgg
     3541 aggtgttcga gcgcatcgag cgcgactacc gggaattcgc ccgacggata ggcctcgatg
     3601 acgtcacctg tatcccgatc tcggcgatcc acggcgacaa cgtcgtgcgg cgcgccgacg
     3661 ccacgcgatg gtaccgcggg ccgacgcttc tcgagcatct cgagaccgtg ccggtgggcg
     3721 aagacctcgc cgccagaccg ttccgcatgc tcgtgcagtg ggtgaaccgg ccgagccagg
     3781 actttcgtgg attcgccggc gtcgtggtga gcggcggcgt tcgcgtggga gaggcagtcc
     3841 ggattctccc ctcgggccgg ccgagcaccg tggcccgagt gctcgtcggt gacaccgatg
     3901 ctcaggcggc ggtcgccggc cagtcgatca cgctgacgct gaccgacgag atcgatatca
     3961 gccgcggcga tgtgctggct gcccccgatg ctgctccgcc ggtcgcggac cagttcgagg
     4021 ccaccgtcgt gtggatgcac gagcagcctc tgctccaggg tcggtcctat ctgatgaaga
     4081 tcggtacgaa gacggtgccg gcgacggtgt ccccgatcaa atacaagctc aacgtcgaca
     4141 cgctcgagca cgtggcggcc aggacgctcg aactgaacga cattggcgtc tgcgccctgg
     4201 agctcgcgca gccggttgtg ttcgagccgt acgcggacag tcgcgacctt ggcgggttca
     4261 tactgatcga tcgcgtcagc aacgcgacgg tgggtgccgg gctgctccgc ttcgcgcttc
     4321 ggcggtcgca gaacgtccac tggcaggcgc tcgacgtcga caaggtggcg cgtgccggtc
     4381 tcaagggcca gaagccgtgc gtcatctggt ttaccgggct ttcgggcgcc gggaaatcca
     4441 ccatcgccaa cctggtcgag aagcggctgc tcgccctcgg ccggcacacc tatctgctcg
     4501 acggcgacaa cgtgcgtcac gggctcaaca aggacctcgg tttcacagac gccgaccgtg
     4561 tggagaacat ccgccgcgtc gccgaggtcg cccgcctcat ggtggatgcg ggcctgatcg
     4621 tgctcgtctc gttcatctcc ccgttccggt cggaacgacg catggcgcgc gagctccttg
     4681 gggaaggtga gttcctcgag gtgttcgtgg acacgccgct cgacgtcgct gaaggacgcg
     4741 atccgaaggg tctctacaag aaagcccggc gcggcgagct gcgcaacttc accggcgtgg
     4801 actcccccta cgagatgccc gagcaccccg acgtgcgagt ggacaccacg cggctgagcg
     4861 cggaggacgc cgcgggcgtg atactggagc ggctcgggcg cacgggcgtc atagacgtcg
     4921 agtagaacgc agccgacgcc cccggctacg ggaggaggat ctcgaacaga cggcgagcga
     4981 caaatgaggc gccggtcacc gacaggtgat cgcggtcgta atacagggtc tgcccgtccc
     5041 gcactgcgta gcagttatca cgatcgcaaa acaggtctgt cggatcgacg acgagcacgt
     5101 ccctgggcag ctccgaaagg tgcgtatcga caaaggcctt gcgttggtcc caccatagac
     5161 gtgtgacgcc gatccggtcg cctcgagagc caggttccat gatgagctct tcgacatgcc
     5221 gcctgagctc gggcgcctgc agcaccaaca cgaccttctt gtgagcgtcc gtgaggcgcc
     5281 cgagaatccg caccagatct ttccagtacg cattgcgcac gttctcttcc accgtgttcg
     5341 gcagccgggg aaacacgccg gcgtgatcgc cgtacaggta caagtggagt cggtagttga
     5401 cgatcacggt gccgattccc gaattgcccg cgaggaacgc cacggcttcg cgcgtccagt
     5461 tgcgacagtc cgcgtcggct gccgtgtgat cgaggacggg tccgcaggcg ctgaatgaga
     5521 gatgcttcac cgtgacgccg acctgtctgc cttgttcggc aacagcgagt gccagctcga
     5581 ctgcgtgact gtcgccgagt acggcccagc ggacggcgcc atcgaaatac tcgcaggcat
     5641 ctgccggttt gagatagtcc cttcctctcg tgtggcactc tggtcgcttc ggactgtttt
     5701 gcgccatgtc cagcagggct cgttgctccg gtgtcgtctt gacgctccgg aagccgtccg
     5761 tgagatatcc agacgtaccc acagagaaga acaggaccga tactccggcg gccgcggcga
     5821 gaaccgtcgg ggctgaccat ccacggaaca gtcgaatggg ctgctcgacg aatcgccaag
     5881 atacgtacgc cagcagcaac gagaggccgc ccagtgcaag catcgagaag gtgcctggtg
     5941 gctcgaacga acgtatgcgt gcgagggcga agagcggatg gtgccacaag tacgcgctgt
     6001 agctgatgag gccgatgacg acgagcggct ttgccgagag aagcctggcc gttcccgtct
     6061 ggcccgccgc aaagagaatc accaacgctg cgccacccac gggaaccaac gcgaacagcg
     6121 atgggaacgg cacagtctcg tcatagagaa cgacggaggt cacgatgagc gcgagaccaa
     6181 gcgcagacag ggcttcgttc ttcaattggc cttgtttgag ctggctcagc gcgcagagca
     6241 cgccgatcga cagctcccag gcacgcgtcg gcaggagata gaaggccgcg gatgcgtcca
     6301 acggctgcca gtactcggcg agtgccagac tcgcgatggt gacggtcacg accagagcgc
     6361 tcaaccagcg aatgccaaat cgccagaaaa gcaggaacgt taccggaaac aagacgtaga
     6421 attgttcctc tacaccgaga ctccaggtgt gcaacagcgg tcgttccgcc gcgagcgggt
     6481 cgaagtaccc gctgtgaaac cagaacagga agttggatgc gaagaccgag accgccgcaa
     6541 cgctttgcga ggagtcccgg agctggccag ggaccatcca cagccacgcg aacggcatag
     6601 tgcacaagat gacgaagaag agggccggca gtattctgcg cgcgcgccgg gcgtagaagt
     6661 cgagaaggga gaatctgcct cgctggtgct ggtcgatgat gatcgacgaa atgaggtaac
     6721 cgctgatcac gaagaacaca tcgacgccga cgaagccgcc gcgagccacc gacagcccgg
     6781 cgtgatagag gatgacgggg aggacggcga cggctcgcaa tccgtccact tcagggcggt
     6841 atctcatgct tcggcgagcg cttggggctc atcccctgac gggtaggtgg gagtttccga
     6901 atgcaatcct gttgccccgc ggtggcgcca gtattgagag gcctctggct cacaactgcc
     6961 aggataatca ccaggcgtca acaggccagt taaggaagtc agccagaagg aaacaaaagt
     7021 ctggggcgga gtagaactat catcatttga gatttctttt ggaagtccta actactatgt
     7081 ttgtagctac ttacggctac agacgccctc taccgtgaag tattcacgcg gcacgattct
     7141 tttataggtc ggtccagagg tctgagtgct cgtgtgcgta ctgtcgccac gcgccttctg
     7201 tgactgacgg ggcgggagct tgccccgata ttcaaaaaca gtggatttca agcgacttcg
     7261 actgattgcc ggggcgtcga tatgcgttct tgtcgcatat gtcggcgtga acgctgcgat
     7321 ggatcgtctg gcccgttggg tggaggtgga acaggacggg tatctctggt ggaaggaaga
     7381 gtggatgcag ctcttcggag cggagaactt cgtcgaccgt ggcggggacc ggatcctgct
     7441 ggccggctca tcggaggttc gcgaagggtt tctgttcgac gagttcgaag ccgagctcca
     7501 aggattcgag gtctacaaca acgcgttctc caaccagacc ctcgagactc tcgtcatcgt
     7561 ccttcaatac atcgagacgg tgtacggccc gtcggcgatg ccccggaaga tcgtggtcgg
     7621 gttcactcct caaacactgt tgaacgtccc gacgctcgaa gcgtcgtact ggccgagagt
     7681 catcgaccgt tacagtccgc aggtgaagct ggatgtcggg agccgaccgg cacgactgat
     7741 ccgcaaggga cgacgcgaag cggtcactgc ccgctaccgg tacttcacgc accaggctcg
     7801 acggtaccag ggagccctgc gtggcgtcat gcgggcgagt atcctcagcg tgtcccctgg
     7861 gatggcggac cgctattggc tgcggctggg gctcgtgcca tccctctacc atcaccttcc
     7921 gcggcgggat cagcgggagc aactgcaggc gttccgacgc gtcctcccgt caccaccgga
     7981 tccggttgca atggcggcga cggtacgagc gcagtgggcc gtgttacggg acttgatggc
     8041 cgcccacgac gtcgagctct atgtggtgaa cgtgcctcaa tcctcccttc tgcgggatga
     8101 ctactacagc gctgtgtacg acgactacga gcaattgctg cgcagcgtcg tgggcaacac
     8161 tccttatctc gacctcgcgc gttatctccg cgacgatgag ttcttcgacc tgacccaccc
     8221 gactctcgcg gccgctcgac ggacctcgag acgtgtcgcg cagttcgtgc gcgaggccga
     8281 cactggacgg ctcccgtacc aggcggcccg acgttccgtt aatgtttccg gacggtcttg
     8341 acgtgcgacc ctctcggtac cctttccgcc aatccctgag atgcccccgg ctgtaaccga
     8401 atctcgtcat ctgtttcgat acgcgaggcc gttcctcgtc ctgatctcga tcgccacgat
     8461 cctgtccgtg ggcgctcgag gcctggtcct ggtcctgccg cagcttgcat cccggatcat
     8521 cgacacggcc cagggtcagc tgcctccctc gctgttgagc acgaccggcg cgctgttgct
     8581 gggagcgctg ttgctggcag cgctacttca gttcgccggc gccgtcatgt tctcctacgt
     8641 cggtgaacga gtcgtgaaga acctgcgata cgacctgttc tcgcacctgc tgtcgctgtc
     8701 gatgagtttc ttcgagcagc ggcgtgtcgg tgaaatgatc tcgagggtcg ccgcggacgc
     8761 gacgacggta cgggagatcg cgacctcact tccacagctg gtcgtgaatc acggcgtgat
     8821 cgctgtcggg gcggtcgcca tcatcttggt ccgccatcca acgctcacgc tcatcatgct
     8881 ggcgttcatg ccgtttatcg cgctcagcgg tgcgatcgcc ggacgtcgcg tgagacgttt
     8941 ggcggtgcgg tcgcaagacg acctcgcgca gacgaacgtc ggcctcgaag aggcgctgtc
     9001 gggcatcgcg acggtgaagg cgttcgctca ggaggagacg gagaccagtc ggtacacgac
     9061 gcggctgtcg gcactcttcc ggtcgtcaat gcgtgtcttc gtcgcacagg agagcatgag
     9121 gctgacctcc ctcctgatcg tgtacggcgg actcgtgacg gtggtctggt acgcgacgac
     9181 acagatcctt gccggcgcgc tcacgacggg tgaactggtg gcgttcatga tgtacgccct
     9241 gttcgccggc aacagcttcc gctcgctcgt cgatgtctgg tcgcgatttg aacgactgcg
     9301 cggcatcacg ctgcggctgg ttcagatctt ccacgagcgc ccatcggtcg tggatcgacc
     9361 ggacgcggtt gggttcgacg gctcccatcg gagaatcgcg ttccacgacg tgagcttctc
     9421 gtatcccacc gatcccggcc ggcaggtcct ggtcgacctc gacttctcct tcgagcgcgg
     9481 tgagcacatc gccctggtcg gagagagcgg cgccggcaag tccaccgtcg ccgcccttct
     9541 gctccgtctc tacgacgtga caggcgggtc gatcacgatc gacggccgcg acattcgcga
     9601 cttgtcgcag aaggaccttc gccgggcgat tgcggtcgtt ccacaggaca tcgtggtgtt
     9661 cgggcgatcc gttcgagaga acatcggcta cggccggcgg gacgcgacgg acgagcagat
     9721 catggcggcg gcgcgcgcgg cgaaggcgct cgagttcatc gaacggcttc cgcagggttt
     9781 cgacacggtg ctcggtgtgc gcggcgtgac cctctcgggc ggacagcgtc agcgcatcgc
     9841 ggttgcccgc gcgatcgtca aggatccggc gattctgatt ctcgacgagg ccacgagcgc
     9901 gctggacaag ccgagcgagg cgctggtgcg cgaggcgctg gcaaatctga tgaagggccg
     9961 cacgaccatc gtgatcgcac accgtctggc cacgatcgag ggcgccgatc gtgtgctcgt
    10021 ccttcacgag ggaagactcg tcgagtcggg cacgccgcac gatctgcttc gcgccggagg
    10081 ccgctacgcc tggcttcaca actcgggagt atgggaagga ggaccggcgg catgagcacg
    10141 ctcgtcgatc ttgtcggacg gcgagcggcc gttgaccccg accggacggc atatcgctac
    10201 ctggacgacg gcgagggcaa cgagcgtcgg ttctcgtacc gtgagctcga cgccagtgcg
    10261 cgggcgattg ctggccgtct gcaggagcag ggagtccgtc agggcgatcg ggtgctgctg
    10321 ctgtacccgt ccgggctcga ctacatcgcg gccttcttcg gctgcctgtt tgcgggggcc
    10381 gtggctgttc cagcgtatgc accgcggccg aaggggcccg atggccgtct ggacgccatc
    10441 gtgaaggacg cgggggccgc ggtcgcgctg acgaccgaag aggtcctgag cgtgatccga
    10501 caactgccgg ctggcgattt ccatcgcgaa gtcaagacgt ggatcgccac cgatcacgtt
    10561 gacgccaacg atgccgggct ctggcggctg ccgaacctgg acgacgacac gctggcgttt
    10621 ctccagtaca cgtcgggctc gacgtcgaca cccaagggcg tcatggtcag ccacggcaac
    10681 ctgatggcca acgagcgcat gctgcaggtg gccctcgaac aggaccagga ctcgacctgg
    10741 gtcgggtggc tgccgatgtt tcacgacatg ggcctgattg gcaacgtcat ccagcccctg
    10801 tacgtcggcg cggcctgcgt cctcatgtcg ccgatggcct tcgtgcagga gccgatccgg
    10861 tggctgcggg ccatcacgaa gtaccgagcc cacacgagcg gggcgccgaa cttcgcctac
    10921 gacctctgcg tggcgaagac gacgcccgag gagcgcgagg ggctggacct gtcgagttgg
    10981 aaactggcct tcaacgggtc agagcccatc cgcgcggaaa ctgtcgatcg gttctctcac
    11041 gcgttcgaac ctttcggctt ccggcgatcc gcgttctatc cctgctacgg gctcgccgaa
    11101 gccacgttgt tcgtatctgg tccggtgaag tcttccgtcc ccgaggttcg cacgttcgag
    11161 gcggccggcc tggagcagcg ccaagcgatc tctacgccgg agggcgacga gggtggtcga
    11221 acgctggtcg gctgcgggcg tacgtggctc gatcagacga tcgtgatcgc agaccccgac
    11281 acatcgaacc ggtgtccgga cggacggatc ggcgagatct ggatctcggg gcccaacgtc
    11341 acgaaaggct actggggtcg cgaagaggcg acgacggcga ccttccgtgc acgtcttgcc
    11401 cacggtggag agggaccgtt cctgcgcacc ggagatctcg gcttctttca cggcggaagc
    11461 ctgttcatca gcggacgcat caaggatctg atcatcattc gaggtcgcaa ccattatccg
    11521 caggacatcg agctgactgt ggagtcgagc caccccatct tgcgccccgg atgcggagcc
    11581 gcattcgcga tcgacgtcga cggtgacgaa cggttggtcg tcgtccagga agtcaagcgc
    11641 aatttcaagg acgcgaacct tcatgacgtg gtcgacgtca tccgcagggc cgtgagcgag
    11701 aatcacgaac ttgcggcgca tgccgtcgtg ctgatcaagc cggggcggat tccgaagacc
    11761 tcgagcggaa agattcagcg gcacgcgtgc cggcagggtt atctcgccgg cgcgctcgat
    11821 ccactcttgt ccagcgcggc ggacgccgcg gccgagggag cgacaccagt caggccggcg
    11881 gcagccagtc tcctgcaaca gacgctcgcc gtgctgggac cggcgggcag ccgccccgtc
    11941 atggagtcgc atctccggca ccgtgtaagc gaggtagccg cggcaaacgg cgccgggctc
    12001 gacgcgatga atgccgaagc caggctccgc ctcgggcgag tcctcgaaac agacctcggc
    12061 gtgtcagtgc cggatgccct cgtgttcgag gcgacaagca tcgaggccct ggccgccaac
    12121 ctgatcgacc tggtcatcgg acgcggcgaa tcgggcgagt cgggcgcccg gccggacccg
    12181 acgcgacgcg tggtcgcata gccactggaa cgaacgtacc gtcaaggaga gctgccatga
    12241 acgattcgaa gcgcgccgca acgcccgagc caccactgcg ccggagagcg cgcccccagg
    12301 ccgtcgcttt ctcggagatg gtcctgacca agaccaggac gctctctccc gagctcacgc
    12361 tgccgctcgt gatcgagccc gcgatcgaca acgtcgatct cgtggcgtgg gcgcgggaca
    12421 acagcgagta cctcgacagg gaactgctga agcacggcgc cctactcctt cgcggcttcg
    12481 agaccaacct cgacgtcttc gaggcggctg ccggcgccgt gtcgaaggag ctgttccggg
    12541 actacggcga cctgccgcag gagcagggca aggacgccgt gtaccactcc acgccgtacc
    12601 ccgaaaacga gatgatcgac ttccacaacg agagctctca tctcaggagc tggccggaga
    12661 agcagttctt catgtgcgtg ctggtttctg aggagggcgg cgtgacgccg attgcggact
    12721 gccgcgaggt gtaccgcctc ctcagcccgg agctgcggga caagttccac tcgaaggggc
    12781 tcaagtacgt ccggaacttc atccccaaac tggacgtgtc ctggcagcag ttcttccgga
    12841 ccgagaaccg ggccgaggtg gaggccaagt gccgggagac tggcacggac ttcacgtgga
    12901 aggacggcga tcggctcaag atcgagagct gggcgcccgg cgtcatcacg caccccagaa
    12961 ccggtgagct ggtgttcttc aaccaggtgc ggctccatca cgtctcgtgc ctcccggccg
    13021 cgtaccgcga gtcgacgctg agcatgttcg ctgaggacga cctgccacgg aacgtggtgt
    13081 tcggtgatgg cacgccgatc accgacgacg agatcgcgca ggtcggtctg gccttcgaca
    13141 aggctgtggt cgacgcccca tggcagaagg gcgacatcat gatgctcaac aacatgctcg
    13201 tcgcgcacgg gcgcaggccg ttcaagggca agcgcaagat cgtcgtggcg atggccgaca
    13261 tgtggaaggt cgaggacgtc caatcgcggc cgaaggcctg aacccttgcc ccctgccctc
    13321 tgccccctgc ccccttcttt acgatgagcc tgctgaagac ggcgttcgcg gcgctggaca
    13381 aggtacagcg cgagcaactg ctggaggctc atctccgcga tcgcgtggca cgggtcctcg
    13441 gtgtcagcgt cgccgaggtg gatgcccagc ggccgctcgg ctcgctgggg ctggactcgc
    13501 tcaaggccgt cgagctgcag cacggtctcg agcgggagct gagtat
//
LOCUS       DBNGSH010000556         2259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1922231, whole genome shotgun sequence.
ACCESSION   DBNGSH010000556 DBNGSH010000000
VERSION     DBNGSH010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1922231"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="AC16CY_12805"
     CDS             <1..865
                     /locus_tag="AC16CY_12805"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3354720.1"
                     /translation="GFASMGESHEEMLRHYEPGGLLPRIVAGLQALGKDLDSVTHEDL
                     APADEFHSAGRSATRALVELAKIPPGSRVLDVGSGLGGPARYLAATLGCDVTGIDLSP
                     EFCGVANALSRMTRLSDRTRFQAGDALELPFAASSFDVVWTIQMQMNIRDKRRLYSGI
                     ARVLRPGGLFVCQEICAGNGEPIELPVPWASRPNQSHLADAESLRELIRGAGLLERTW
                     RDITADIVAARKAQQAKAQASGASGSSAPPPLGMHLVLGEQAAVKMSNSGRNTDAGRT
                     VFIQGVFDKPG"
     gene            complement(1021..1311)
                     /locus_tag="AC16CY_12810"
                     /pseudo
     CDS             complement(1021..1311)
                     /locus_tag="AC16CY_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1366..1641)
                     /locus_tag="AC16CY_12815"
     CDS             complement(1366..1641)
                     /locus_tag="AC16CY_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354721.1"
                     /translation="MSRLVIGTDVIRVERQRAGLHQAEHHHVRAVLRVDVLTELQAAP
                     AAVGTPALDRQHHPGVGLIGMQIAGRHTPGKVTIAACTSMSTPNDGQ"
     gene            complement(1773..2048)
                     /locus_tag="AC16CY_12820"
                     /pseudo
     CDS             complement(1773..2048)
                     /locus_tag="AC16CY_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083047449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          367 a          717 c          780 g          395 t
ORIGIN      
        1 aggatttgcg tccatgggcg agtcgcacga ggaaatgctt cgtcactacg agccgggcgg
       61 acttctgccg cgaatcgtgg cggggttgca agctctcggc aaggacctgg actcggtcac
      121 gcacgaagat ctcgcgccgg ccgacgagtt tcactccgcg ggacgttcgg ccacgcgcgc
      181 attggtggag cttgcaaaga ttccgccagg atcgcgagtg ctcgacgtgg gcagcgggtt
      241 gggcggcccg gcgcgttacc tcgccgcgac tttgggttgc gacgtgacag gcatcgatct
      301 ctcgcctgaa ttctgcgggg tggccaacgc cctttctcgg atgacgcggt tgtccgatcg
      361 cacccgcttt caggcgggtg atgccctgga gcttccgttc gccgcgtcca gcttcgacgt
      421 ggtgtggacg atacaaatgc agatgaacat ccgggacaag cgccgcctgt attccggaat
      481 cgcccgggta ctcaggccgg gcgggctgtt cgtctgccag gaaatctgcg cggggaacgg
      541 cgagccgatc gagcttccgg tcccatgggc gagccgcccc aaccagagcc atctggccga
      601 tgcggaatcc ctccgcgagt tgatccgcgg tgcaggtctc ctggagcgca cctggcgcga
      661 cattaccgcc gacatcgtgg cggcgcgcaa ggcacagcag gcgaaggcgc aagcgtcggg
      721 tgcgagcggc tccagcgccc ctcctccgct tggaatgcat ctcgtgctgg gagaacaggc
      781 agccgtcaag atgagtaact ccgggcgcaa caccgacgcc gggcgcacgg tgttcatcca
      841 gggcgtattc gacaagcctg gctagtggtc cgatccgctg atcccgctgg aaaactcggc
      901 cgaaaatcaa aggccccgcg cgacgctgaa acgggaggca aatcgatcgc ttgtcgtccg
      961 ttgttcacag ggagtgatct cggcgatcgc gcgttcggac gcaaaggagt tgctcggcga
     1021 tcaacgcggt gatcttgtgg cggtcatgtc gatcggcacg atgcccgccg actggctgac
     1081 gtcgacgacg agacgaatgc cggcctcgcg acatcgcttg ccgatctccg cgaccggctg
     1141 cacggtgccg agcacgttcg acgcgtggtt gatgatgacc atgcgagtgt gcttgcggaa
     1201 cgcgcgcttg acgtcgtcgg gatcgatgta tccggcggca tcgttggcga cgtgcgtgac
     1261 ctcgatatca ccgtcgcggg ctcggtggcg cagcgggcgc agcaccgagt tgtgctcgag
     1321 cctgcgtctg gcctgggttc acttcgccac ccccgggccg ggccgctact ggccgtcgtt
     1381 cggtgtagac atcgacgtgc aggcggcgat ggtgaccttc cccggggtgt ggcggccagc
     1441 gatctgcatg ccgatgaggc caacaccggg gtgatgttgg cgatcgagtg ctggtgtgcc
     1501 aacggctgcc ggggccgcct ggagttccgt cagcacgtcg acgcggagga cggcgcggac
     1561 gtggtggtgc tcagcctggt ggagaccagc cctctgccgt tcgacccgga tgacatcggt
     1621 cccgatgacg agcctggaca cgacggcctg gtcctcaccg gggcacggac ccgaacgagc
     1681 cgcccttctg acaggcggcg cctgcggcaa ccaacgacgc cgggcggcgg cttcagcagc
     1741 gaagtcaccg cccggcgtcg gtcttggatg gctcaggcat cctcggtggc ggtggccttg
     1801 ggcacgcggc cgaggtcgcg gttgcgcaac ctgtacgaat cgcccttgag cgagatgacc
     1861 tcggcatgat ggaccagccg gtcgatcatc gcggcggcga ccacgtcgtc gccgaacacc
     1921 tcaccccagc ggccgaacgg tttattgctc gtgacgatca agctggccct ctcataccgg
     1981 gccgagacca gctggaagaa caggttcgcc gcctccgcct cgaacgggat atatccaacc
     2041 tcgtcgacct atgccgacct cggcataggt cgacttatgc cgaccccggg actatgccga
     2101 ccggccggtc gcgtgagccg gcgatccctt gccccggtgg gcttcgcgtg taccggctcg
     2161 tcatcgtggt cggcataatc acagcgcctc gaggaacgcc agcagcgggt cgggtgggcg
     2221 gtatcgacct ggcggcgagg tggtcggtgt gactcgggc
//
LOCUS       DBNGSH010000557         3984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_734289, whole genome shotgun sequence.
ACCESSION   DBNGSH010000557 DBNGSH010000000
VERSION     DBNGSH010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_734289"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="AC16CY_12825"
     CDS             <1..773
                     /locus_tag="AC16CY_12825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR3354722.1"
                     /translation="VLSTMTTTAVEQVNEARGEPVWFQLYHRDDWGQTRELLKRVESA
                     GCPALVFTVDLLGGRNMEQFNRVFRQNRQQCAACHVNGEPLTDLSHRPMVNALAKPAT
                     PRPEIGTPTWDYVKRLKDATGMKLLVKGIVTREDAELAIQNGVDGVYVSNHGGRAENS
                     LRATIECVADVAAGVKGRAPVLVDGGFRRGTDVFKALALGATAVGVGRPYMWGLASFG
                     QEGVEAVLDILRRELQLIMRQSGTTSVAAIGSNYIVQR"
     gene            933..1214
                     /locus_tag="AC16CY_12830"
     CDS             933..1214
                     /locus_tag="AC16CY_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3354723.1"
                     /translation="MSEAMMTVEQARRALGVSRATVWRLIQRGELPSVRRSGRRLVQA
                     SAIRTRTVRRSTRDIPAFSHDHPIFRLVGAGRGGGRTPGARDKHAVLDR"
     gene            1211..1618
                     /locus_tag="AC16CY_12835"
     CDS             1211..1618
                     /locus_tag="AC16CY_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3354724.1"
                     /translation="MTRSVLWDSSAVLALLDADDADHARAVSVARDIASDRRPSFITN
                     YVEAEAHALLVRKLGRVVARQWLLTGGLPVVRVLPSEEQRAREILARYADKDWTLCDA
                     ISFAVLDVRRIRRAFTFDHHFRQYGRIQVLGLE"
     gene            1645..1806
                     /locus_tag="AC16CY_12840"
     CDS             1645..1806
                     /locus_tag="AC16CY_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354725.1"
                     /translation="MSGSMDTIVFATMARIAQLVYQTVFSIANDERALVRDNKGPRTG
                     FGSKADVIR"
     gene            1871..2440
                     /locus_tag="AC16CY_12845"
     CDS             1871..2440
                     /locus_tag="AC16CY_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354726.1"
                     /translation="MLVYGAIAVFGFLVLLILLVAGDFSGDHDIGGHDIGGHEAPGDL
                     GHDGGGMSVFSVRVMASFVTAFGVGGLVARFYGLSHPLSSGVGAVSGVAMAGLVYQFA
                     RVLYSQQASSEIRMAGLVGRSAEVSVAIPVSGVGQVSLTYGGERSEHIARTSDGRALP
                     RGTAVVITSIRGDSVVVGPSSDAPTGVSR"
     gene            2437..3957
                     /locus_tag="AC16CY_12850"
     CDS             2437..3957
                     /locus_tag="AC16CY_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIR3354727.1"
                     /translation="MNFFGIEFSAIAVALAILLGLLGIFAAMALFARNYIKVPPSMVA
                     IFYGRKHTIVDDRGGRSTVGFRVIRGGAALRVPVLEEVQYLSLNIISIPLKISRAYTK
                     EGVPVTVEAVANVKIAGDDMSLRGAAERFLGMTTDKIKDVIFLTLEGHLRAILGTLTV
                     EEINADRQAFAQKMTDEAAVDLKKMGVNIDILTIQQISDEQGYLDALGKKRTAEVKRD
                     AIIGEAQATRDAMIKSALADQEGKTKRYEADVAIALSLRDKETRQAEFDAAVQAKQAE
                     SEQAGPLATAIAKQKVTEQETRIDQVRKTQEVLVQEQEAARREKELQATVIKPAEAER
                     QASILRAEGEKQSTIIRAEATQKELEFEGAGEAAKIERIGRAEAAKVLAVGEAEAEVI
                     KKKLLAEAEGLQSKAEAWKNFNEAAVINLVVDKMPELAQAFATQLAGIDKINIIEMGN
                     GSGGAGGVGKVMSTVGGGMTAMLGMLKDQFGIDVARLMQAKTEAAADEAERKTGKQ"
BASE COUNT          721 a         1245 c         1401 g          617 t
ORIGIN      
        1 aggtcttgtc gacgatgacg acgaccgcgg tggagcaggt gaacgaggcg cgtggagagc
       61 ccgtgtggtt tcagctgtat caccgggacg actggggcca gacgcgcgag ctgctgaaga
      121 gagtcgaatc agcgggctgc ccggcgctgg tcttcaccgt ggacctgctg ggcggccgca
      181 acatggagca gttcaaccgc gtcttccgcc agaaccggca gcagtgcgcc gcgtgtcatg
      241 tgaacggcga accgctcacg gacctgagcc accggccgat ggtcaatgcg ctcgcgaagc
      301 ccgcgacacc gcggccggag atcgggacgc ccacctggga ctacgtcaag cgcctgaaag
      361 acgcgaccgg catgaagctg ctcgtcaagg ggatcgtcac gcgcgaagac gcggagctgg
      421 cgatccagaa cggcgttgac ggcgtgtacg tctccaatca tggcgggcgt gccgagaaca
      481 gcctgcgcgc gacgatcgag tgcgtggccg acgtggcggc cggcgtcaag ggccgcgcgc
      541 ccgtgctggt ggacggcggc ttcaggcgcg gaacggacgt cttcaaggcg ctcgcgctcg
      601 gtgcaacggc cgttggcgtg ggccgcccgt acatgtgggg cctggcctcg ttcggtcagg
      661 aaggcgtcga ggccgtgctc gacatcctgc gccgggaact gcagctcatc atgcggcagt
      721 ccggcacgac ctccgtcgcc gccatcggct cgaactacat cgtccagcga tagagccgtt
      781 tggtgggttg gctgaccctt cccgggttcg taggtgaacg taatccggct cgcgtcacca
      841 tccagggaca tgtgaaagcg cctcgtccag gaccggtgcg cgcccgagcg tgagacgatt
      901 gagatggtga gacagtcatg atacgatgag ccatgtccga ggcgatgatg acggtggaac
      961 aagctcgccg tgcgctcggc gtctcgcgag cgacggtctg gcggctcatc cagcgcggtg
     1021 agttgccgag cgtccgccgc agtgggcgcc gcctcgtaca agccagcgcg atccgcacgc
     1081 gcacggtccg ccgcagcacg cgcgatatcc cggccttctc acacgatcat ccgatcttcc
     1141 gcctcgtcgg cgccgggcgg ggcggcggca ggacacctgg cgcacgcgac aagcacgcgg
     1201 tcctcgaccg gtgacccgga gcgtcctctg ggactcgagc gccgtcctgg ccctgctgga
     1261 cgcagacgac gccgatcatg cccgggccgt ctccgtggct cgcgacatcg cgtccgacag
     1321 gcggcccagc ttcatcacca actacgtcga agccgaagcg cacgcgctgc tcgtgcggaa
     1381 actgggaagg gtggttgccc gtcagtggct gctgacgggc gggttgccgg tggttcgtgt
     1441 gcttccctcg gaggagcagc gagccaggga gattctggct cgatacgccg acaaggactg
     1501 gacgctgtgc gacgcgatct cgtttgccgt gctcgacgtc cgccggatca gacgggcgtt
     1561 caccttcgac caccacttcc ggcagtacgg ccggattcag gttctcggcc tggaatgaac
     1621 tggcctccag cggacgatca caacgtgagc ggctcgatgg acacgatcgt cttcgcgacg
     1681 atggcgcgca tcgcgcagct cgtctatcag accgtctttt cgatcgcgaa cgacgagcga
     1741 gcgctcgtgc gcgacaacaa ggggccgcga accggcttcg ggtcgaaggc agacgtgatc
     1801 cgctgacggt caaggaaccc tcccgccgct cattgacaac ctcggcggcg agaatgtagc
     1861 gtcacctcgc atgctcgtct acggggccat cgcggtcttc ggcttcctcg tgctcctcat
     1921 tctgctcgtt gccggcgatt tctctggcga tcacgacatc ggcggccacg acatcggcgg
     1981 ccacgaggcg ccgggcgacc tcggacacga cggcggaggc atgagcgtct tcagcgtccg
     2041 cgtcatggcg tcgttcgtga cggcgttcgg cgtcggtggc ctcgtcgcgc gcttctacgg
     2101 gttgtcgcat ccgctgtcgt ccggcgtggg cgccgtgtcg ggcgttgcca tggcggggct
     2161 cgtgtaccag ttcgccaggg tcttgtattc acagcaggcc tcgagtgaga tccgcatggc
     2221 ggggctggtc ggccggtccg ccgaggtgtc ggtggcgatt ccggtgtctg gcgtgggcca
     2281 ggtgtctctc acctatggcg gcgagcgatc ggagcacatc gcgcggacct ccgacggccg
     2341 ggcgctgcct cgcgggacgg ccgtcgtcat caccagcatc agaggcgatt ccgtcgtcgt
     2401 gggcccttcg agcgacgcac ctactggagt gtcccgatga acttcttcgg catcgaattc
     2461 tccgcgatcg ccgtggcgct cgcgatcctg ctcgggctgc tgggcatctt cgcggccatg
     2521 gcgctcttcg cgcgcaacta catcaaggtg ccgccgtcga tggtggcgat cttctacggg
     2581 cgcaagcaca ccatcgtcga tgatcgcggc ggccgctcca cggttggctt ccgggtcatc
     2641 cgcggcggcg cggcgctgcg cgtcccggtg ctcgaggagg tgcagtatct ctccctcaac
     2701 atcatctcga tcccgctgaa gatctcgcgc gcctacacga aggaaggcgt gccggtcacg
     2761 gtggaagcgg tcgcgaacgt gaagatcgcg ggcgacgaca tgtcgctgcg cggcgcggcg
     2821 gagcgattcc tgggcatgac gaccgacaag atcaaggatg tcatcttcct gacgctcgaa
     2881 ggccacctgc gggcgattct cggcacgctc accgtcgagg aaatcaacgc cgaccgccag
     2941 gccttcgcgc agaagatgac agacgaagcc gccgtcgatc tcaagaagat gggcgtgaac
     3001 atcgacatcc tgacgattca gcagatcagc gacgaacagg gctatctcga cgccctcggc
     3061 aagaagcgca ccgccgaagt caagcgcgac gcgattatcg gcgaggcgca ggcgacgcgc
     3121 gacgcgatga tcaagtcggc gctggcggac caggagggga agacgaagcg ctacgaggcc
     3181 gacgtcgcga tcgcgctgtc gctgcgcgac aaggagacga ggcaggccga gttcgacgcc
     3241 gccgttcagg cgaagcaggc cgaatccgag caggccggac cgctggccac ggcgatcgcg
     3301 aaacagaagg tcaccgagca ggagacgcga atcgatcagg tgcgcaagac gcaggaggtg
     3361 ctggtccagg agcaggaggc ggcgcgaagg gaaaaggagc tgcaggcgac cgtcatcaag
     3421 cccgccgaag ccgagcggca ggcgtcgatc cttcgagcag agggcgaaaa gcaatccacg
     3481 atcatccggg ccgaggccac gcagaaggag ctggagttcg aaggcgcagg cgaggccgcg
     3541 aagatcgagc ggatcggcag agcggaggcg gccaaggtcc tggccgtcgg cgaagcggaa
     3601 gccgaggtga tcaagaagaa gctgctcgcg gaagccgaag ggctccagag caaggcagag
     3661 gcgtggaaga acttcaacga ggctgccgtc atcaatctgg tcgtcgacaa gatgccggag
     3721 ctggcgcagg cgttcgcgac gcagcttgcc ggcatcgaca agatcaacat catcgagatg
     3781 ggaaacggat cgggcggcgc cggcggcgtc ggcaaggtga tgagcactgt gggcggcggc
     3841 atgacggcga tgctcgggat gctgaaggat cagttcggga tcgacgtcgc ccggctgatg
     3901 caggccaaga cggaggccgc cgccgacgag gcggaacgca agaccggaaa acagtgagat
     3961 ggccaccgat gccgtctacg agag
//
LOCUS       DBNGSH010000558         2854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2941443, whole genome shotgun sequence.
ACCESSION   DBNGSH010000558 DBNGSH010000000
VERSION     DBNGSH010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2941443"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC16CY_12855"
     CDS             <1..411
                     /locus_tag="AC16CY_12855"
                     /inference="COORDINATES: protein motif:HMM:NF042488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HIR3354728.1"
                     /translation="SHVISMCIFAGLIVMMDLRLLGVGNMRTPFSEVQKRLFPWQMTG
                     MTLSAITGLTLTYADPMRFYVNIFFWTKMLVMVLAGVNALAFHFITYNSVVKWDSAET
                     TPFGAKLSGAVSIVMWAIVIVSGRLIPYNWFQST"
     gene            427..894
                     /locus_tag="AC16CY_12860"
     CDS             427..894
                     /locus_tag="AC16CY_12860"
                     /inference="COORDINATES: protein motif:HMM:NF042488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HIR3354729.1"
                     /translation="MLQPFFEWLGALPISQFIGSSIWIYPLVQAFHLVCLALLAGAIL
                     VVDMRLLGLGLTTRPVSQVARDARPWLIAGLIGMVCTGLPQLLQNASREYFSEYFWYK
                     MYLIAFGLILMVTVRRKATQAAEPSAFGKVVAVVSIAAWGGAVWNARLIGLFT"
     gene            941..>2854
                     /locus_tag="AC16CY_12865"
     CDS             941..>2854
                     /locus_tag="AC16CY_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3354730.1"
                     /translation="MPLTAGTRLGAYEILSSLGAGGMGEVYRARDTKLDRAVAIKILP
                     EAFAADTARIARFEREAKTLASLNHPNIAHIHGLEESRGVRALVMELVEGEDLAQRIA
                     RGAIPVAEALLIARQVAEALEAAHEQGIVHRDLKPANIKVRPDGTVKVLDFGLAKAME
                     PTGVASPSVSQSPTITTPAMTEAGMILGTAAYMSPEQARGTPVDKRSDIWAFGCVLFE
                     MLAGRPAFAGETTAETLAGVLHHEPPWAALPSETPADARIVVSRCLEKDLKQRFRDIG
                     DVRLALAGAFTSDAAAAVPRPAVGALPLWRRAVPVVAASVIVAAVTAIAMRLQESRPA
                     LQVSRWSLEATGPTALNVNGVEHDLTITPDGSRVIYVGDRGRGIFVRALDNLEPRPLV
                     KGTATLRSPFVSPDGRWVGFEEDLTSLKKVPIAGGPAIPVAQIDGILRGAAWLPDNTI
                     VFATNRIAGLQRVSAEGGKPATLTKPDDARNEFAHHWPQLLPDGSALLYTALARTGGL
                     DAAKVRLLDLETNRSIDVLDGASNALYVSSGHLAYVAGATLWAVPFDLDRRATFGTAV
                     ALSNQVVTTTNGAGLFAVSTTGTLVYAHASGFDPLARTLNWIDRQGRVEMIPAPPHAY
                     AQPRLSHDGTRVVA"
BASE COUNT          455 a          928 c          962 g          509 t
ORIGIN      
        1 tcgcacgtga tcagcatgtg catcttcgcg ggactgatcg tgatgatgga cctgcggctg
       61 ctcggcgtcg gcaacatgcg cacgccgttc tcggaggtac agaagcggtt gttcccgtgg
      121 cagatgacgg ggatgacgct cagcgccatc accgggctga cgctgaccta cgccgacccg
      181 atgcggttct acgtgaacat cttcttctgg acgaagatgc tcgtgatggt gctcgccggc
      241 gtcaacgcgc tggcgttcca cttcatcacc tacaactccg tcgtcaagtg ggattcggcg
      301 gaaaccactc cattcggggc gaagctctcg ggtgcggtgt ccatcgtcat gtgggccatc
      361 gtcatcgttt cgggccggct catcccgtac aactggttcc agagcacctg atccggagtc
      421 gcacgaatgc tgcagccgtt cttcgagtgg cttggcgccc tgccgatcag tcagttcatc
      481 ggcagctcaa tctggatcta cccgctcgtg caggccttcc acctcgtgtg cctcgcgctg
      541 ctcgcgggcg ccatcctggt cgtggacatg cggctgctgg gcctggggct gacgacgagg
      601 cccgtgtcgc aggtggcgcg cgacgcccgg ccctggctga ttgcggggct catcggcatg
      661 gtgtgcacgg gactgccgca gctgctgcag aacgcgtcgc gcgagtactt cagcgagtac
      721 ttctggtaca agatgtactt gattgccttc gggttgatcc tcatggtgac cgtccgccgg
      781 aaggccacgc aggccgccga gccgagcgcg ttcggcaagg tcgtggccgt cgtctcgatc
      841 gccgcgtggg gcggggcggt gtggaacgcc cggctgatcg gtctgtttac ctaaagcgcg
      901 taacaaacta ggagcgggcc gctagaatca gggtcgcccc atgcctctaa ccgccggcac
      961 acgcctcggc gcgtacgaga tcctgtcttc gctcggcgcc ggcggcatgg gtgaagtcta
     1021 ccgcgcgcgg gacacgaagc tggatcgcgc cgttgcgatc aagatcctgc cggaggcgtt
     1081 tgccgccgac acggcgcgga tcgcgcggtt cgagcgcgag gccaagaccc tggcctcgtt
     1141 gaaccacccg aacatcgccc acattcacgg tctcgaagaa tcacgtggcg tccgcgcgct
     1201 cgtgatggaa ctcgttgagg gcgaggacct cgcacagcgc atcgcgcgcg gggcgatccc
     1261 cgtcgccgaa gcactactca tcgcgaggca ggtggctgag gcgctggaag cggcgcacga
     1321 gcaaggcatc gtccatcgcg acttgaagcc ggcgaacatc aaggtgcgac cggacggtac
     1381 ggtgaaggta ctggacttcg gcctggcgaa ggcgatggag ccgacgggcg tcgcatcacc
     1441 gagcgtctcg cagtccccaa ccatcaccac acccgcgatg accgaggcgg ggatgatcct
     1501 tggcacggcc gcctacatga gcccggagca ggcgcgtggc acgccggtgg acaagcgttc
     1561 cgacatctgg gcgttcggct gcgtgctgtt cgagatgctg gcggggcgac ccgcgtttgc
     1621 cggcgagacc actgccgaga cgctggccgg ggtgctccat catgaacctc cgtgggcggc
     1681 gctgccctcc gagaccccgg ctgacgccag gatcgtggtg tcgcgttgcc tcgaaaagga
     1741 cctgaagcag cgctttcggg acatcggtga tgtccggctg gcgctggcag gcgcgtttac
     1801 gtcggatgcg gctgccgccg tgccgcgacc ggctgtcggc gcgctgccct tgtggcgacg
     1861 cgctgttccc gttgtggctg cctcggtcat cgttgcggcg gtcacggcaa ttgcgatgcg
     1921 ccttcaagag tcgcgtccgg cgcttcaggt ctcgcggtgg tcgctcgagg ccacgggtcc
     1981 caccgcactg aacgtgaacg gcgtcgaaca cgatctgacc attactcccg atgggtcgcg
     2041 tgtgatctac gttggcgacc gcggccgggg gatcttcgtg cgagcgctcg acaacctgga
     2101 gccgcgaccg cttgtcaagg gaaccgccac gctgcgaagc cccttcgtgt cacctgatgg
     2161 ccgatgggtg ggttttgaag aggatctcac gtctctaaag aaggtgccga tcgccggcgg
     2221 gccggcgatc ccggtggcac agatcgacgg gatcctcagg ggcgccgcct ggctgccaga
     2281 caacacgatc gtgttcgcga ccaaccgaat cgccgggttg cagcgtgtgt cggcagaggg
     2341 cggcaagccg gcgacgttga cgaagccgga cgatgcccgc aatgagttcg cccatcactg
     2401 gccccagctc cttccggacg gcagcgctct cctgtacacg gcgctcgccc gcaccggcgg
     2461 gctcgacgcg gcgaaggtcc gcctcctcga tctggagacc aacaggtcca tcgatgtcct
     2521 tgacggagcc agcaatgccc tgtacgtgtc cagcggacat ctggcctacg tcgcaggagc
     2581 gacgctgtgg gcggtgccgt tcgatctgga ccggcgcgcg acgttcggca cggcggtcgc
     2641 gctatccaac caggttgtca ccaccaccaa cggtgcgggt ctgttcgcgg tatccacaac
     2701 cggcacgctg gtctacgcgc acgccagcgg cttcgatcct ctggcgcgca ccttgaactg
     2761 gatcgaccgg cagggccggg tggagatgat tccggcgccg ccgcacgcct atgcgcagcc
     2821 gcgactgtcc cacgacggta cacgcgtggt cgcc
//
LOCUS       DBNGSH010000559         5204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2602132, whole genome shotgun sequence.
ACCESSION   DBNGSH010000559 DBNGSH010000000
VERSION     DBNGSH010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2602132"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="AC16CY_12870"
     CDS             <1..249
                     /locus_tag="AC16CY_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354731.1"
                     /translation="NEMYGERVTSFDFKASKIIRFGNRRVTIGGDLYNVFNSDAVQNY
                     DNTYTLDNPATPANEQAWGTPTGLISPRFWRISASFDF"
     gene            complement(646..1074)
                     /locus_tag="AC16CY_12875"
     CDS             complement(646..1074)
                     /locus_tag="AC16CY_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354732.1"
                     /translation="MLKTSLPLAQVLATPTVDGRITIDLPSGTRITIPVADLHIDLSK
                     ALEAARANQTAAPAQTAPSDPTSIINRKCEAEWKTDFQMQAFCRKRQLEAAATLSKRS
                     MTSPNEQAIRKQCATEWTEDFSMWNFCEERQLKALKELGR"
     gene            complement(1275..2231)
                     /locus_tag="AC16CY_12880"
     CDS             complement(1275..2231)
                     /locus_tag="AC16CY_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3354733.1"
                     /translation="MVKVRVLVMLTFCGLVAAGLASSTGVLRAQANQLQFVVGATDEK
                     GEPVTDLKPEEVIMSENGKPGKTVSLEPFHIPVKLTIGVDNSPDSREALSHYRNGLKA
                     MVEGLPPEIEVTLITTAPQPRMVVRPTTNREQILRGINGFAPGVDTEDRPRFTDTMVE
                     YSQRLEKEAKGGKGIDFVPVLLMISTTANEQTSYQVPEVEKALTFLGLRKARVMVVMT
                     STKTGDATAVADLNTNRQAIIAIPVTKSTRGRYESIGVSSRLVTLLPEFGKDIAALHA
                     RHYNQYKVTVERAGGLTGPLQNPTVELTRTSVKGSVSPDGLP"
     gene            complement(3002..4711)
                     /locus_tag="AC16CY_12885"
     CDS             complement(3002..4711)
                     /locus_tag="AC16CY_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3354734.1"
                     /translation="MTRVAALLIAIGGAISAQTPSVGESPVRFSDITRQAGIDFRHIS
                     GTSADKHLVETIGSGGLFFDFDNDGWIDVFLVDGGSVADPALAKQARHRLYRNRGNGT
                     FEDVTERSLIRHRDYGMGACAGDYDNDGWVDLYVTNVGPNVLYRNGGNGVFADVTRTA
                     RVGSPLWGTGCAFADLDRDGDLDLFVTNYVAADPKDSPFCGNARLKTRFYCHPLNYDP
                     LPNVVYRNDGNGAFTDISAQSGVAAYRANGLGVVITDYDDDLWPDVFVANDSMPNFLF
                     RGTDGTRFVETALRAGVAVATDGKARAGMGTDTGDYDGDGQIDLVVTNLDLETFSLYR
                     GLGGGLFAYATPESGIGPATLPFVGFGAVFLDFDNDTGLDLAFANGHIFDNAPEFRPG
                     ATYAQRNLLFRYLAPRRFVEVGRNAGPGFALEKVSRGLVSGDIDNDGDLDLLVTNNGQ
                     TADLLRNDGGNGGNALLVRAIGGSASLTASKKSNRDGVGARLRLKVGTRTLIRDVKAG
                     SSYLGQNDLRQHFGLGTAKSADRLEVRWPSGRTDVLQNLPANQIITIREGAGLVNSVP
                     FVR"
     gene            complement(4846..>5204)
                     /locus_tag="AC16CY_12890"
     CDS             complement(4846..>5204)
                     /locus_tag="AC16CY_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354735.1"
                     /translation="LADRRRNMTLDVLREEAALRAAEGAYDRAAALWQQVIDQQPDRS
                     SDHLGLAAALAGAGRIDAAIREYEAAAQLDGAPDVYRQLAAAYARAGRTEDSARARIM
                     YDRALQGLPTGQGPRP"
BASE COUNT          832 a         1712 c         1725 g          935 t
ORIGIN      
        1 aacgagatgt acggcgagcg ggtgacctcg ttcgatttca aggcgtccaa gatcatccgg
       61 ttcgggaaca ggcgcgtgac gatcggagga gacctgtaca acgtgttcaa ctccgacgcg
      121 gtccagaatt acgacaacac ctacaccctc gacaaccccg caacgccggc caacgagcag
      181 gcgtggggca cgccgacggg cctcatctct cccaggttct ggcgcatctc ggcgtcgttc
      241 gacttctgat cgtcgaactc aggggtacac aagaaatccc gcgggtcagc gagtcccgcg
      301 ggctttttcg tgtaccccca gttcgctctt ctcgcttcac acttctcagt tcgtgttcag
      361 tttcggggtt cgtgttcgag gttcgaggtt cgtggctcgg cggcaacggg ctaccagcta
      421 caggcgagaa gaggtaggga aacgtgcgct ttggctcgag gcgtgattgg cacctcgaag
      481 cccggaacat cgaacagcga actcagaaca cgtgatcgcc ttcccgctcg gcccgcggac
      541 gatccagcag ggctcgagca gcgtcggcgc cggcggccgc gggacgatgg ttccaagtgg
      601 cccagtccaa cccggtcaag ctcctggtgc agacgtctgc actcgctaac gcccgagttc
      661 cttgagggct ttcagctgtc gttcctcgca gaaattccac atcgagaagt cctcggtcca
      721 ttccgtcgcg cactgcttgc ggatcgcctg ctcgttcggt gatgtcatcg atcgcttcga
      781 gagcgtggcg gcggcctcca gttgtcgttt tcggcagaag gcctgcatct ggaagtcggt
      841 cttccattcc gcctcgcact tgcggttaat gatcgaggtg ggatccgacg gcgccgtctg
      901 cgctggcgcg gctgtctgat tcgcgcgcgc ggcctccagg gccttcgaca agtcgatgtg
      961 gagatccgcg acggggatgg tgatccgcgt gcccgatggc aagtcgatgg tgatgcgacc
     1021 gtccaccgtt ggtgtcgcca gcacctgcgc cagcggcagc gatgtcttga gcaccactta
     1081 ctgcgcgtgg accgatgtcg cggtgagcac accgagcagc acgacgagtc gcttcagcat
     1141 atcgggtccc tccatcacag catccccgcc tctctccatg ggggcggcag ttggcccgct
     1201 agtgatcaac gcgaacctga aactgaacac gaacagagaa cggagaagcg agaagcgtga
     1261 actacaggtt caccttacgg cagcccgtca ggcgagactg agcctttcac cgacgtgcgg
     1321 gtgagctcca cggtggggtt ttgaagcggc ccggtcaagc cgcctgcccg ctcgaccgtg
     1381 accttgtact ggttgtagtg ccgcgcgtgc agcgccgcga tgtccttgcc gaactccggc
     1441 aacagcgtca cgaggcggct cgaaaccccg atcgactcgt agcggccccg cgtcgacttc
     1501 gtaacgggga tcgcgatgat ggcctgacga ttcgtgttga gatccgccac ggcggtcgcg
     1561 tcaccggtct tggtcgaggt catcaccacc atcacgcgcg ccttccgaag cccgagaaag
     1621 gtcagcgcct tctcgacctc gggcacctgg tagctggtct gctcgttggc cgtcgtcgag
     1681 atcatcagca gcacgggcac gaagtcgatg cccttgccgc ccttggcctc cttctcgaga
     1741 cgctgcgagt actccaccat ggtgtcggtg aagcgcgggc ggtcctccgt atcgacgcca
     1801 ggggcgaacc cgttgatgcc acgcaggatc tgttcgcggt tggtcgtcgg ccgcacgacc
     1861 atgcgcggct ggggcgccgt ggtgatgagc gtgacttcga tctcgggcgg cagcccctcc
     1921 accatcgcct tcaggccgtt ccggtaatgg ctcagcgcct cgcggctgtc gggcgagttg
     1981 tcaaccccga tggtcagctt gacgggaatg tggaagggct cgaggctgac ggtcttcccg
     2041 ggcttgccgt tctcgctcat gatgacctct tcgggcttga ggtccgtcac cggctctccc
     2101 ttctcatcgg tggcgccgac cacgaattgc aattgattcg cctgagcgcg caggacgcca
     2161 gtcgacgacg ccagcccggc agccacgagc ccgcagaacg tgagcatgac caacactcga
     2221 accttcacca tgacagcccc tccgaaaagt ggccgatgat accactccca atccccaact
     2281 cccaatctcc aacgcccaac tccgaatcaa cttccaaacc tcgtgggcgc ttgggaattg
     2341 ggagttggga ggggtacgtt tagcgaggcg catgtgctca ccacgggcag gcatgccggc
     2401 cggcggccgc gggcgctgcg gccgggaaac cggctctccg ctcataccct gccgccgcca
     2461 cgtcgactaa acgctgtctc gaggcatggt ccgtcacgcg gcacaaaacg cccgtgtcgc
     2521 cacgcgcttc tcaagcgcgt ggacggaagt cgacgtgagg aatcgcgtcg cccggtttcc
     2581 ggcttcgcgg cgtggtcggc cttgcttacg cgtccgctgt ccggcacgcc tgcccgtggt
     2641 gagtggcgcg cgcccggtgg gcgacctgcg cgaggagacg cggaccgggc cgcgaccgcg
     2701 tacgccaggc cgcaggcagt gcgcagcagg aaagtggccg gcgcgattca gccgcgcgtc
     2761 ttccggccat gcgttcgagt tgacggaatc aggctcggag gttttgagcc ggcggcggat
     2821 gtcgcctcgc attggcgagt agacgcgcgg ggagcgattg ggtccgagcg ccggtcactt
     2881 tcccggccgc cgcgtccgcg gtcgtggccc ggtctgcgtc tccgccgcgc agccgaggcc
     2941 acggttgcga gcggtgcgaa cccgctaaac aaagacgttg ggagttggga gtcgaggcac
     3001 gtcagcggac aaacggcacg ctgttcacga ggcccgcgcc ctcgcggatg gtgatgatct
     3061 gattggccgg caggttctgg agcacgtcgg tccgcccgct gggccaccgc acttcgagac
     3121 ggtcggcgct cttcgccgtc ccgagcccga aatgctgccg caggtcgttc tgtccgaggt
     3181 agctcgaccc ggccttgacg tcgcggatca gcgttcgtgt ccccaccttc agtcgaagcc
     3241 ttgcgccgac gccgtcgcga ttgctctttt tgcttgcggt gagcgaagcc gaaccaccga
     3301 tcgcgcggac gagcagcgcg ttccccccgt tgccgccgtc gttgcgcagg aggtcggctg
     3361 tctggccgtt gttggtcacc agcaggtcca gatcgccgtc gttgtcgatg tcgccggaca
     3421 cgagcccccg gctcactttc tccagcgcga agcccgggcc ggcgttgcgg ccgacttcga
     3481 cgaaccgcct tggggcgagg taacggaaca ggaggttgcg ctgcgcgtac gtcgccccgg
     3541 ggcggaactc cggggcgttg tcgaagatgt ggccgttggc aaaagcgaga tcgaggccgg
     3601 tgtcgttgtc gaagtcgagg aacacggcgc cgaacccgac gaaggggagc gtcgccgggc
     3661 cgatgccgct ttctggcgtc gcgtacgcga atagaccgcc gcccagcccg cgatagaggc
     3721 tgaacgtctc gaggtcgaga ttggtcacga ccaggtcgat ctgcccgtcg ccgtcgtagt
     3781 ctccggtgtc ggtgcccatc ccggcgcgcg ccttcccgtc ggtggcgacg gcgacgcccg
     3841 cgcgcagcgc cgtctcgacg aaacgcgtgc cgtcggtgcc gcgaaacagg aagttcggca
     3901 tgctgtcgtt ggccacgaac acgtccggcc acaggtcgtc gtcgtagtcc gtgatgacga
     3961 ctccaaggcc gttggcccga taggcggcga cgcccgactg cgcgctgatg tcggtaaacg
     4021 cgccgttgcc gtcattgcga tagacgacgt tcggcagcgg atcgtagttg agcgggtggc
     4081 agtagaagcg cgtcttcagc ctcgcgtttc cgcagaaggg gctgtccttc ggatcggcgg
     4141 cgacgtagtt ggtcacgaac aggtcgagat cgccgtcgcg atcgaggtcc gcgaacgcac
     4201 acccggtgcc ccacagcggc gatcccaccc gcgcggtgcg cgtcacgtcg gcgaacaccc
     4261 cgtttccgcc gttgcggtac aggacgttcg ggccgacgtt ggtgacatac aggtctaccc
     4321 agccgtcgtt gtcgtaatcg cccgcgcacg cccccatgcc gtagtcgcga tgccggatga
     4381 gcgatcgttc ggtgacgtct tcgaacgtgc cgttgccccg attccggtac agccggtggc
     4441 gcgcctgctt cgcgagtgcg ggatcggcca ccgatccacc gtccaccagg aagacgtcga
     4501 tccatccgtc gttgtcgaag tcgaaaaaga gcccgcccga gccgatcgtt tcgacgaggt
     4561 gcttgtcggc gctcgtgccg ctgatgtgac ggaagtcgat gccggcctgt cgagtgatgt
     4621 cggagaagcg cactggcgac tccccgacgg acggggtctg ggcgctgatc gctcctccga
     4681 tcgcgatcaa cagcgcggcg acacgcgtca ccgcgccgtg ccccgagggg accgctatgc
     4741 ggctcgttgc gtggaatttg tgccttttgt cactcgcaga gcggtcccgg cgcgcgcagc
     4801 gcgccaagag acactgccaa ccggccaagg gcttcggccg ggacatcaag ggcggggccc
     4861 ttggccggtt gggagtccct gaagggcccg gtcgtacatg attcgcgcgc gcgcgctgtc
     4921 ctcggtgcgc ccggctcgcg cgtacgccgc ggcgagctgg cggtacacgt ctggcgctcc
     4981 gtcgagctgg gcggcggctt cgtactcacg gattgccgcg tcgatccgtc ccgcacccgc
     5041 aagagccgcg gcgaggccga ggtgatcgga ggaccggtcc ggctgctgat cgatcacctg
     5101 ctgccagagc gcggcggcgc gatcgtaggc gccctcggcg gcacgcagcg ccgcctcttc
     5161 cctcagcacg tcgagcgtca tgttgcgccg ccggtcggcg agca
//
LOCUS       DBNGSH010000560         7335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_905019, whole genome shotgun sequence.
ACCESSION   DBNGSH010000560 DBNGSH010000000
VERSION     DBNGSH010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_905019"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="AC16CY_12895"
     CDS             <1..209
                     /locus_tag="AC16CY_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354736.1"
                     /translation="RLRNQIEDLLFKILPLDAEPPDPSLDDAELVNIVAQNLEMPEAT
                     RQDLLERPNALERARALVALLAIK"
     gene            350..1525
                     /locus_tag="AC16CY_12900"
     CDS             350..1525
                     /locus_tag="AC16CY_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354737.1"
                     /translation="MRTTIRRLLVGSCLAWPLLAGVEARAQSGLSEAILALANGPGSS
                     IREVDGRLFGAIVDATIINRNNDDSDFAFDDEINFQISRGNDSLVFDLADRTRTGFEQ
                     WARDHATQLLQIIFPSSLSSATLGRDAAQLYAQQLMLATILDVDPPGEPGSPRRSHAG
                     GLVEFEWFGQANSPGDPGGTAWQGLYSFGRFVSVQGRYASRRDGLATSGWAASADYHP
                     YKEIPDLPVVLRVGASARAGFLYSRSSSLTPSSNSIDLGTIDVGGGGWASVRKDFDRF
                     RVGGGTLFQGTRSTVPFSNEEWGFIAELIEERGMSWDISYGGTIAVDTTRSAAVIGKY
                     LETRPVAASFDRPVLRMGLIGYSFALGPGTALNAGYKIVSSGGVRSGGVFLQGHFGW"
     gene            1537..3009
                     /locus_tag="AC16CY_12905"
     CDS             1537..3009
                     /locus_tag="AC16CY_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3354738.1"
                     /translation="MQARRRRVLCVTAALAVLTAADARAQQGIPPMKARVLVGAAGEF
                     DADLQYYDPSDLFLRVKDAQVLPFWVSAKNASPQPALISPRAFTLRVGNGKAVTPLAA
                     MDASAAEDALKKATSLNPLLRILAAQGCEYCPDPLRRAFREETVRPGETVSGWIYFPR
                     PAGLDFNGFMEFVSTGHRPSLLPTASVAISGPPEGTRAGPRLPAAVVRAINAATKIGE
                     EIRYGSPPFERSVAVLFGISEYDRRNDTWAHRDLENMSKALTAQGFDPVIKVANRDVT
                     ADTLRNIQGHFKNDLRPDDRLLVYYSGHGERSPKGDTGYIVLAASNPGASSRKTDVSM
                     TEFMSWMRNLPVKHLLVILDACYSGLAIGDRSKGQGPVLDALTRQQLYELSSRSGRFV
                     ITAGDDAQLAHEHNRWDGGLFTRGLLQALATRDGSRRPDNRLVTAWELFVRAKEYVRE
                     QVRTYRLSPQAPLFRDLGEAPSPDPPRVVSRGEFVFVNMP"
     gene            3024..3521
                     /locus_tag="AC16CY_12910"
     CDS             3024..3521
                     /locus_tag="AC16CY_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354739.1"
                     /translation="MSEILGALLTMSVLALGAGLTYRMVARGQTRRLALAVVLGLAIA
                     VVTLIVPGRAETKGDISEIASVVFCYLCMLAGMMAEYFYKQGERGRRRFKFELVTFLM
                     PIFASPIVFIPLLTLTTDDALAGGAFTRAKLMVYLVAFQNGFFWRNFFEQRRLPPIPE
                     VNPGT"
     gene            complement(3627..4367)
                     /locus_tag="AC16CY_12915"
     CDS             complement(3627..4367)
                     /locus_tag="AC16CY_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR3354740.1"
                     /translation="MKLKLITVLAVFTLASSASAQDLPNCSAQGLPNHALLTSTLQSV
                     LGANGGFNLPMWASIISRRGEVCIVTRSGGRGEQWPGSRVISAQKANTANAFSLPGLA
                     LSTANLYTAVQPGGSLYGLQHSNPVDPAVAYAGNANTYGGPGDPMVGSKIGGVNVFGG
                     GLALYNENGTLVGAIGVSGDTSCADHVIAWKVRDALGLDHVPGGVSGDDDRPDNIIYD
                     IGEPMPSGFGHPHCLPSDVDPASLPTAP"
     gene            complement(4571..6604)
                     /locus_tag="AC16CY_12920"
     CDS             complement(4571..6604)
                     /locus_tag="AC16CY_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3354741.1"
                     /translation="MRKTLLALSAALVSITASPAAQQAPRATFRSDVELVVVDVVVRD
                     RSGNAVRGLTAADFEVREDNRPQQIVSFDVEEVTTERAPDSPGPPVLAVGTQGVTTER
                     APAPEPEPVKREDLAGRRLIVLLFDLSSMQPEELTRAGKAAVEYLDTQMAEADLVAVA
                     TVDVALNVVADFTGDREQVKSAMARFSAVDGVAFETPAAETAATDEAAAETAATSAEY
                     DLFNNDARLRAIRTLVEMLGTIEQKKSVLYFSGGMSRSGGDNQVELRAATSAAVRANV
                     SLYPVDTRGLQATVPGGDASRPSARGVGAFSGRGVQQQFNSLFASQETLQTLAADTGG
                     RAFTDTNNFGDAFAQVQRDTSIYYLLGYNSTATANDGRFRRISVRVKRSDLRVDHRAG
                     YYAERDFSHTGRQDRERQLQEQLTSPVSATDLPVVVSAGWFRMAPDRYYIPLSVAVPG
                     SALREGRDRNTLDVMGLIRDEQGRSVGRMRETITLASSGSAGLATQQVLYQSGLMLPP
                     GRFAVKVVVRENVDGRMGSFETGVFVPDLRQAPVKVSSVTLSTQVRPAGDKRSASPLV
                     REGLELLPSLTHVVDRTQKMYFYYEVYDPQVADGGSALLKTSLAFYRGAVKVFETPLV
                     ERSDLDAADRRAALFQFEVPAEAFKPGLYTCQVNIIDDVAGRFAFPRLALYVR"
     gene            complement(6604..>7335)
                     /locus_tag="AC16CY_12925"
     CDS             complement(6604..>7335)
                     /locus_tag="AC16CY_12925"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3354742.1"
                     /translation="EGSSLLHAGTIRLRRQQSRGIAGQINYTIARSMDDASSIGGGGR
                     VVAQNDRDLEAEWGRSSFDQLHQLSGNLSVELPFGANRQWLNQGGPWAAMLTDWTLSA
                     SFTAASGNPFTARILGAASDVARGTYGTLRADYSGDQIGIDDPDLLQFFNTSAFALPP
                     PGRFGSAARNTIVGPANMQFDASIGRDVRLTNNQTISIRVQGTNLLNTVRFGGIDTTV
                     NSPTFGQVVSIRPMRSVGLNVRFRF"
BASE COUNT         1176 a         2472 c         2465 g         1222 t
ORIGIN      
        1 gcaggctgcg caaccagatc gaggatttgc tcttcaagat actgccgctc gacgccgagc
       61 ccccggatcc ctcgctcgac gacgccgagc tcgtcaacat cgttgcgcag aacctggaga
      121 tgccggaggc aacgcgacag gatctcctcg agcggccgaa cgccctcgaa cgcgcccgcg
      181 cgctggtggc gctgctcgca atcaagtagc ggccagtcgt agtgtccggc ttcagccgga
      241 ccaataatgt acgcttcccc acctagctga gctgtctgtt cgatccctcc tgagcgggcg
      301 tttatatcag tggcaactga aagcggcgtc ggggtgcggg agggctgcca tgagaacaac
      361 cattcggcga ctcctcgttg gttcgtgtct cgcgtggcct ctgctggccg gcgtcgaagc
      421 gcgggcccaa agcggtctct ctgaggcgat cctggcgctc gccaacggac cggggagcag
      481 catccgggag gtcgacggcc gcctgttcgg cgcgatcgtc gacgccacga tcatcaatcg
      541 gaacaacgac gacagcgact tcgccttcga cgatgagatc aactttcaga tctctcgggg
      601 gaacgattcg ctcgtattcg acctggccga ccgtacgcga accggattcg agcaatgggc
      661 gcgtgaccac gcgacgcagc tgcttcaaat catttttcct tccagtctgt cgtcggcgac
      721 tctcggccgc gacgcggcgc agctctacgc ccagcagctg atgctcgcca cgatcctcga
      781 tgtcgatcct ccgggcgagc cggggtcccc ccgccgatcg catgcgggcg gcctggtcga
      841 gttcgagtgg ttcgggcagg ccaactcgcc cggcgacccc ggtggcacgg cgtggcaggg
      901 tctgtattcg tttggccggt tcgtctcggt gcagggacgg tatgcgagcc ggcgcgacgg
      961 tctcgcgacg agcgggtggg ccgcgtcggc ggactatcac ccgtacaagg agatcccgga
     1021 cctccccgtg gtcctgcgcg tcggcgcctc ggcgcgcgcc gggtttctgt actcccgatc
     1081 gtcttcgctg acgccctcct cgaacagcat cgatctgggg acgatcgacg tcggcggcgg
     1141 cggatgggcg tcggtgcgca aggacttcga ccggttccgt gtcggcggcg gaacgctctt
     1201 tcagggaaca cgcagcacgg tgccgttctc caacgaggaa tggggcttca tcgcggagct
     1261 catcgaggag cgcgggatga gctgggatat cagctacggc ggcacgatcg ccgtcgatac
     1321 gaccaggtcg gctgccgtga tcggcaagta cctcgagacg cggcccgtgg ccgcgagctt
     1381 cgatcggccg gtgttgagga tgggcctcat cggctactcg ttcgcgctgg gtccgggcac
     1441 ggccctgaac gccggctaca agatcgtgtc cagcgggggc gtgcgatcgg gcggcgtgtt
     1501 cctgcagggt cacttcggct ggtaggggcg gcggccatgc aggctcgacg acgtcgtgtt
     1561 ctgtgtgtga cggcggcgct cgccgttctc acggccgccg acgcgcgcgc gcagcagggc
     1621 atcccgccga tgaaagcgcg cgtgctcgtc ggcgcggcgg gggaattcga cgccgacctg
     1681 cagtactacg atccgtccga tctcttcctg cgcgtcaagg acgcgcaggt gctgccgttc
     1741 tgggtctcgg cgaagaacgc gtcgccgcag ccggcgctca tctcgccgcg cgcgttcacg
     1801 ctccgcgtcg gcaacggaaa ggccgtcacg ccgctcgcgg ccatggacgc gtccgccgcg
     1861 gaagacgcgc tgaagaaggc caccagcctg aacccgctcc tcaggatcct ggcagcgcag
     1921 ggttgcgagt actgccccga cccgctgcgg cgcgcctttc gcgaggagac ggtgcggccc
     1981 ggcgagacgg tgagcgggtg gatctatttt ccgcggccgg ccggcctcga cttcaacggc
     2041 ttcatggagt tcgtctccac gggccaccgg ccgtcacttc tgccaaccgc gtcggtcgcc
     2101 atcagcggtc cgccggaggg gacgcgggcc ggaccgcgcc ttcccgccgc ggtcgttcgt
     2161 gccatcaacg cggcgaccaa gatcggggaa gagatcagat acggctcacc cccgttcgaa
     2221 cggagcgttg cggtgctctt cggcatctcc gagtacgacc gccgcaacga tacctgggcg
     2281 caccgcgacc tggagaacat gtcgaaggcg ctcacggctc aaggattcga tccggtcatc
     2341 aaggtcgcca acagggatgt caccgccgac acgctgcgca acatccaggg gcatttcaag
     2401 aacgaccttc ggccagacga tcggctgctc gtctactact cgggccacgg cgagcgatct
     2461 ccgaagggcg acacgggcta catcgtcctc gccgccagca atcctggtgc gtcgtcccgc
     2521 aagaccgacg tttcgatgac cgagttcatg tcgtggatga ggaatctgcc cgtgaagcat
     2581 ctgctcgtca ttctggatgc ctgttacagc ggcctggcca tcggcgaccg gtcgaagggt
     2641 caggggccag tcctcgatgc tctgacccgc cagcagctct acgagctcag cagccggagc
     2701 ggccggttcg tcataaccgc cggcgacgac gcgcagctcg cgcacgagca caaccggtgg
     2761 gacggcgggc tcttcacgcg cggactgctg caggcgcttg ccacccgcga tggtagccgc
     2821 cggcccgaca acaggctcgt caccgcgtgg gagctctttg tacgcgcgaa agagtatgtc
     2881 cgcgagcagg tcaggacgta ccggctcagc ccgcaggccc ctctgtttcg ggatctgggc
     2941 gaagcgccgt cacccgaccc gccgcgtgtg gtgagcaggg gcgagttcgt cttcgtcaac
     3001 atgccgtaac acaggagcca gacatgtcag agatcctcgg tgccctcctg acgatgtcgg
     3061 tgctcgcgct cggcgccggt ctcacgtacc ggatggtggc gcgcggccag acccgccgcc
     3121 tcgccctggc ggtggtcctc ggtctcgcca ttgccgtcgt cacgctgatc gttccggggc
     3181 gagccgagac gaagggcgac atcagcgaga tcgcctccgt cgtcttctgc tacctgtgca
     3241 tgctggccgg catgatggcc gagtatttct acaagcaggg cgagcgaggc agacggcggt
     3301 tcaagttcga gctcgtgacg ttcctgatgc cgatcttcgc gtcgccaatc gtcttcatcc
     3361 cgcttctcac cctgacgacc gacgatgcgc tggccggcgg ggcattcacg cgcgcgaagc
     3421 tgatggtcta cctcgtggcg tttcagaacg gcttcttctg gcgaaacttt ttcgagcagc
     3481 gccggctgcc ccccattcca gaggtgaacc cgggaacgtg agggatgccc cccgtcagcg
     3541 cacgcggagc gagaggcggt cggctcgggt caagaggcgg gtccccgatc ggacccgcct
     3601 caattcccga ccgtgcgagt cctgactcag ggcgcagtcg gcaacgatgc cggatcgacg
     3661 tccgaaggaa ggcaatgagg gtgtccgaat ccgctgggca tcggctcgcc gatgtcgtag
     3721 atgatgttgt cgggccgatc gtcgtcaccg ctcacgccgc cgggcacgtg atccaggccc
     3781 aacgcgtctc ggaccttcca cgcgatgacg tgatcggcgc acgatgtatc gccgctgact
     3841 ccgatcgcgc cgacgagcgt gccgttctcg ttgtagagcg caaggccgcc gccaaacacg
     3901 ttgaccccgc caatcttgct cccgaccatc ggatctccgg ggccgccata cgtgttcgcg
     3961 ttgccggcat acgccacagc cgggtcgact ggattgctgt gctgcagtcc gtacagcgac
     4021 ccgccgggct ggacggcggt gtagaggttg gcggtcgaca acgccaggcc cggaaggctg
     4081 aaggcgttgg ccgtgttggc tttctgcgcc gagatgaccc tgctgcctgg ccactgctcg
     4141 ccgcgcccgc ccgaacgcgt cacgatgcac acctcgccgc gacggctgat aatgctcgcc
     4201 cacatcggga ggttgaagcc cccgttggcc ccgagcacgc tctgcagcgt cgatgtcaac
     4261 agcgcgtgat tcggcagtcc ctgtgccgag cagttgggaa gatcctgcgc ggacgcagac
     4321 gaagcgagag tgaacaccgc cagtaccgta atcagtttca gtttcatggt tgacctccag
     4381 gacaggccgg cccgaatcgc actcgggcct tgtttctccg cgcacgtgac gagaaggcgt
     4441 cccgcacgcg tcgccgggaa gtattatccg atcgcgcgtc ggcggggtaa cggccgttta
     4501 tcgtgaggaa gatcggactc gcactgacgc gacttcagac gcaccggcag cgaccggaac
     4561 tcgctccccg tcaacgcacg tagagcgcga gacgcggaaa cgcgaaccgc cccgcgacgt
     4621 cgtcgatgat gttcacctgg caggtgtaga gaccgggctt gaacgcctcg gccggcacct
     4681 cgaactggaa gagggcggcg cggcggtcgg ccgcatcgag gtcggatcgc tccacgagcg
     4741 gcgtctcgaa caccttcacg gcgccgcgat agaacgcgag gctcgtcttg agcagggcgg
     4801 acccgccgtc agcgacctgc ggatcgtaga cctcgtagta gaaatacatc ttctgcgtgc
     4861 ggtccacgac gtgcgtgagg ctcggcagca gctcgagccc ctcgcggacg agcgggctcg
     4921 ccgatcgctt gtcgccggca ggacggacct gcgtgctgag cgtgaccgag ctgaccttga
     4981 ccggcgcctg acgcaggtcc ggcacgaaca cgcccgtctc gaaggaaccc atccggccgt
     5041 ccacgttctc gcgcacgacg accttcaccg caaacctgcc aggcggcagc atgagcccgg
     5101 attgatagag gacctgctgc gtggcaaggc ccgccgagcc gctcgaggca agcgtgatcg
     5161 tctcgcgcat ccggccgacg gaccgtccct gttcgtcgcg aatgagaccc atcacgtcga
     5221 gggtgtttcg atcgcgcccc tcgcgaaggg ccgacccggg cacggcgacc gagagcggga
     5281 tgtagtagcg gtccggcgcc atccggaacc agcccgccga gacgacgacg gggaggtcgg
     5341 tagccgagac gggtgaggtg agctgctcct gcagttgacg ctcgcggtcc tgccgcccgg
     5401 tgtggctgaa gtcgcgctcg gcatagtacc cggcgcgatg atcgacgcgg aggtcggacc
     5461 gtttcacgcg aaccgagatc cggcggaagc ggccgtcgtt cgcggtggcc gtgctgttgt
     5521 agccgagcag gtagtagatc gaggtgtctc gctggacctg cgcgaacgcg tcgccgaagt
     5581 tgttcgtgtc ggtgaaggcg cgtccgccgg tgtcggccgc cagggtctgc agcgtctcct
     5641 gggaagcgaa caggctgttg aactgctgct gtacgccgcg tcccgagaac gcgcccacgc
     5701 cgcgcgcgct cggtcgagac gcgtcaccgc cgggcaccgt cgcctgcagc ccccgcgtat
     5761 cgaccggata cagagacacg ttggcacgca cggccgcgct cgtcgccgcc cgcagctcga
     5821 cctggttgtc gccgccgctg cgcgacatcc cgccgctgaa gtagagcacc gacttcttct
     5881 gctcgatcgt gccgagcatc tccacgaggg tccggatggc gcgaaggcgc gcgtcgttgt
     5941 tgaacaggtc gtactcggcg ctcgtggccg cggtttcggc cgcggcctcg tcggtggcgg
     6001 cggtctcggc cgccggcgtt tcgaacgcga cgccgtccac ggcgctgaac cgcgccatcg
     6061 ccgacttcac ctgctcgcgg tcgccggtga agtcggcgac gacgttcaac gcgacatcga
     6121 cggtggcgac ggccaccaga tccgcctccg ccatctgcgt gtcgaggtac tcgaccgccg
     6181 cctttccggc gcgcgtcaat tcctccggct gcatcgaact caggtcgaac aacagcacga
     6241 tgagccggcg ccccgcgaga tcctcgcgct tgactggctc gggttcgggc gccggggcac
     6301 gctcggtcgt cacgccctgc gttcccacgg cgagcaccgg cgggcccggc gagtcgggcg
     6361 cgcgctccgt cgtcacttcc tcgacgtcga acgagacgat ctgctgcggc cggttgtcct
     6421 cccgcacctc gaaatccgcg gccgtcaggc cgcgaacggc gttgccggac cgatcgcgga
     6481 ccaccacgtc cacgacgacg agctcgacgt ccgatcggaa cgtggcccgg ggggcctgct
     6541 gtgccgccgg ggatgcggtg atcgagacga gcgccgccga gagagccagg agggtcttgc
     6601 gcatcagaac ctgaacctca cgttcagccc gacgctgcgc atcggccgga tcgagacgac
     6661 ctgcccgaag gtcggcgagt tcaccgtcgt atcgatgccg ccgaaccgga ccgtgttgag
     6721 caggttggtc ccctgcaccc ggatcgaaat cgtctgattg ttcgtcagcc ggacgtcgcg
     6781 tccgatcgac gcgtcgaact gcatgttggc cggcccgacg atcgtgttgc gcgcggcgct
     6841 gccgaaccgg ccgggcggcg ggagggcaaa tgccgacgtg ttgaagaact ggagcaggtc
     6901 gggatcgtca atgccgatct gatcgccgct gtagtcggcg cgcagcgtgc cgtacgtgcc
     6961 gcgcgcgacg tccgaggcgg ccccgaggat ccgcgccgtg aacgggttgc cggacgcggc
     7021 cgtgaacgag gcggagagcg tccagtcggt cagcatcgcg gcccacgggc cgccctgatt
     7081 cagccactga cgattcgcgc cgaacggcag ctcgaccgac aggtttccgg agagctggtg
     7141 cagctggtcg aagctcgagc gtccccattc ggcctcgaga tcgcgatcgt tttgcgcaac
     7201 cacgcgaccg ccgccgccga tcgacgaggc atcgtccatc gacctggcga tcgtgtagtt
     7261 gatctgcccc gcgatgccgc gcgactgctg ccgccgcaga cggatggtcc cggcgtgcag
     7321 gagcgagctc ccctc
//
LOCUS       DBNGSH010000561         7721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2772671, whole genome shotgun sequence.
ACCESSION   DBNGSH010000561 DBNGSH010000000
VERSION     DBNGSH010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2772671"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="AC16CY_12930"
     CDS             complement(<1..1139)
                     /locus_tag="AC16CY_12930"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354743.1"
                     /translation="MNAVGFAWRSLVRQPARAGLGVLGVAAVGALLFDMLLLSRGLIV
                     SMEDLLERGGWDVRVSAADLPGSGLRLPNARETTSAITALPVVQAALMIRGATAQIDH
                     ASGRPLRATFQGVAAHGSPRGGTRLRAPWTILRGRDAEHPGEVVIDEALAAEGYGIGR
                     DVTVRATCVADLESLPPTRLAVVGIAEFPFATNAHTIGGTFDALETACGGNIGDEADL
                     ILVRSTGDASGTAAAIRAARPNVRAATNSDVVGRLQQTGFTYFRQISTVLATVTVGFA
                     VLLITVLLTVSVNQRIGEIAALRALGFSRARVVKDVLSESALIVGIGGLASLPLGLLL
                     ASLLDRILKSLPGIPAALHFFVFEPRTLWIHVGLLTATAIVAAAYP"
     gene            complement(1136..1987)
                     /locus_tag="AC16CY_12935"
     CDS             complement(1136..1987)
                     /locus_tag="AC16CY_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354744.1"
                     /translation="MKCGSILLALALAASPGLASAQQRAIPDILISRHLAEAERLAVG
                     STVTLASEVAGTQPREFRVAGIYEPTPDPERLGVAPLELRMHLPDLLDMTRPIDVPAG
                     AEYVTSINVALTDPDDAAAFARDVNVRVPGIVARPAGDATGSAGTFRVLERFHLAIAV
                     VTIVAATVFLLALSIMLVDERRETVGVLRLIGLPVRRVLLQILLEGLLIAAAGAAFGL
                     VLAVISQKAINDFFQWRYDTALVFVRVTKDVALTCITIAVPLGASATVLASWALLRRS
                     GLRLARR"
     gene            complement(1984..2400)
                     /locus_tag="AC16CY_12940"
     CDS             complement(1984..2400)
                     /locus_tag="AC16CY_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR3354745.1"
                     /translation="MAQWLVKEEPEHYGYDKLEKDGKTVWAGVKNPLAQKHLRAIRSG
                     DRIFYYHTGKEKAVVAIARAASHAYPDPRDRSEKLSVVDVVPEKKLTRPVTLKEIKAD
                     KAFASFPLVRMSRLSVMPVTDAEWARIEAISRSDRS"
     gene            2518..3312
                     /locus_tag="AC16CY_12945"
     CDS             2518..3312
                     /locus_tag="AC16CY_12945"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3354746.1"
                     /translation="MPHSTTRQGIIATTSGAITGVAAGFIGVGGGEFRIPVLVSLLGF
                     PLRLAGGVNLVVGLFTVALGVLRRWGQQPLTRDDLILVGIMGVVSLAGAAAGVLGRKR
                     MPVRPLRVVFCAYLIVAGLWMLYESIAHVEHVLLEPTGMLRWTLAAAMGFAIALVSGV
                     LGVAGGEMRIPALLYLFGVPIVEAGTLSLAVSIPTVAAGAFTDHRTGGIPRPAMRLAL
                     LMGIASAVGVLAGAALVPYASREVIKGVLGVVLLLATVRLAVGPSH"
     gene            complement(3325..6420)
                     /locus_tag="AC16CY_12950"
     CDS             complement(3325..6420)
                     /locus_tag="AC16CY_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3354747.1"
                     /translation="MKQLIQWSIDHRWIVLGLSILLAAAGAWTAWTMPVDVFPDLTAP
                     TVTILTEGRGMAPEEMETLVTFPIEAAINGASDVRRVRSATAVGIAVVWVEFDWGTDI
                     FRARQVVAEKLALVGGSLPPQVEPPILAPISSIMGEILFFAISSKQEDPLALRTIADT
                     LVRRRLLAVAGVSQVTSIGGAERQYQVIAHPDQLRANGISLSELLDGVRGASQNTSAG
                     IYMEGPQEYVLQTIGRVRTPEEIGDSVVALRGDRSVLIRDIADVREGAALKRGEGSRN
                     GEPAVIVGVQKQPDANTVELTARLDRELDSLQRELPPGMTIDRLIFRQSDFIEVAVGN
                     VIAALRDGGLLVIAIVVLFLANMRAAAITLTAMPLSLAAAVLVLRFFDATINTMTLGG
                     MAIAIGALVDDAIIDVENVVRRLRENAARAPADQRPAADVVRDATLEIRTSIVFATII
                     IVLVFLPVFGLSGVEGRLLSPLAFAYIVALLASLGVAIVVTPALSCAFLPGARSILRG
                     HDGWLARVLKARFALALPRALDHPRLVAAASVVLLFAAAAAMTRMGTAFLPEFHEGSL
                     TIQANTLPGTSLAKSDEIGRRIEQILLAQREVRATARRTGRAEYDEHVQGVEAAEIDV
                     GLREAERPRAELLAELRRGFSTLPGTNVTIGQPISHRIDHMLSGTRANIAVKVFGEDL
                     PTLRRLGERVRDVMTAVPGAVDVSLEQQTDVPFVRFVLNRPAIARYGLRASDVAEAVE
                     TALAGAVVGRIFDRATAVALVVKFNPASQADFDRLADLPIDTPNGGPVPIRLLADVRR
                     ETGPNMVLRENVQRRIVISSNVAGRDLGSVVDDMRSAIGRDVPMPPGYRVEYGGQFES
                     QQSAAERLLLLGAAVVAGLFMLLVLAFGRPRDALLVMLNLPLALIGGVAGVYLAGGVL
                     SVASMIGFITLFGIATRNGIMLVSHIRHLTEHEGVTDFREAVERGARERLIPILMTAM
                     AAGLALIPLALGGGKAGSEIQTPMAIVILCGLTTSTLLNMVVVPTMYLRYASGGSWRR
                     G"
     gene            complement(6429..>7721)
                     /locus_tag="AC16CY_12955"
     CDS             complement(6429..>7721)
                     /locus_tag="AC16CY_12955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3354748.1"
                     /translation="ESGGAPRALTGPEPSRPGAFRIEEAPPAPGRYRWALVLEGAALS
                     DRHDLGVITIFADEATARADAEKQPAEDPSAIAYLKEPQWTNPFATAVVREGEVRTSV
                     RAPATIHPLPGGEAIVAAPASGRFQADTLLSIGERVSPNQILGRLEPRLASGADRATL
                     DADAAEARAGLEAARVEQGRAERLLADRAVPARRVEDARRAVAVAEARLRAADARLAQ
                     RDETLRTGGGAAAGNAFALRAPIAGRLAEVSATLGAAYEEGAPLFRIVRTDRVELEVQ
                     VPAADVAAARQTAGLALEIPGLPAPLTLDPQHVHDAGVIDPRTRALPLQMEIANPGEQ
                     LLIGQVGTAVLFGRERRRVPVVPAAAVLMEAGRPYVFVQTGGERFARRFIEIASRDGD
                     LVGIRSGVTPGERVVTRGAYDVQLASAARGLPAEGHVH"
BASE COUNT         1172 a         2707 c         2697 g         1145 t
ORIGIN      
        1 gggtacgcgg cggcgacgat cgcggtggcg gtgagcaggc cgacgtgtat ccacagcgtc
       61 ctcggctcga acacgaagaa gtgcagcgcc gccggaatgc cgggcaggct cttcaggatg
      121 cggtcgagca gcgaggccag cagcagcccg agcggcagcg acgccagccc gccgatcccg
      181 acgatgagcg ccgattccga gaggacgtcc ttgaccacgc gtgcccgcga gaagccgagc
      241 gcgcggagcg ccgcgatctc gccgatccgc tggttgaccg acaccgtcag cagcaccgtg
      301 atgaggagca cggcgaaccc gacggtgacc gtggcgagca ccgtcgagat ctggcggaag
      361 taggtgaagc ctgtctgctg gagacggccg acgacatcgc tgttggtggc cgccctgacg
      421 tttgggcgcg ccgcgcggat cgccgccgcg gtgcctgacg cgtcgcccgt cgatctgacc
      481 aggatgagat cggcctcgtc tccgatgttg ccgccgcatg ccgtttcgag cgcgtcgaat
      541 gtccccccga tcgtgtgggc attggtggcg aacgggaatt cggcgatgcc gacgacggcg
      601 aggcgcgtgg gcgggagcga ctccagatcg gccacgcacg tcgcccgtac ggtgacgtcg
      661 cgtccgatgc catacccttc ggcggcgagc gcctcgtcga tgacgacctc gccggggtgt
      721 tccgcatccc ggccgcgcag gatcgtccac ggcgcgcgga gccgcgtccc gccgcgcggg
      781 gagccgtgcg ccgcaacgcc ctggaacgtc gcgcggagag gacggccgga cgcgtggtcg
      841 atctgggcgg tcgctccgcg gatcatgagc gccgcctgca cgacaggcag cgccgtgatc
      901 gcgctcgtcg tctcgcgcgc gttcggcagg cggagcccgc tgcccggaag gtccgcggcg
      961 ctcacgcgga cgtcccaccc gcctcgctcg agcagatcct ccatcgacac gatgaggccg
     1021 cgcgacagca gcagcatgtc gaagagcagc gcgccgacgg ctgcgactcc cagcacgccc
     1081 agcccggcgc gcgccggctg ccggacgagg ctgcgccacg caaaccccac cgcgttcatc
     1141 gtcgagccag gcgcaggccg ctgcggcgaa gcaaggccca cgacgccagg accgtggccg
     1201 acgccccgag cggcaccgcg atcgtgatgc aggtgagcgc cacgtccttc gtcacgcgga
     1261 cgaagacgag cgccgtgtcg taccgccact ggaagaagtc gttgatcgct ttctgcgaga
     1321 tcaccgccag caccaggccg aacgccgcac ccgcggcggc gatgagcagc ccctcgagca
     1381 ggatttgcag gagcacgcgg cgcacgggga ggccgatcag gcgcagcacg cccaccgttt
     1441 cgcggcgctc gtccacgagc atgatcgaga gcgccagcag gaacaccgtc gccgcgacga
     1501 tcgtgacgac ggcgatcgcg agatggaagc gctcgagcac gcggaacgtg cccgccgatc
     1561 cggtcgcgtc gcccgccggc ctggcgacga tcccggggac gcgcacgttg acgtcccgcg
     1621 cgaacgcggc ggcatcgtcc gggtcggtca gcgcgacgtt gatcgacgtc acgtactcgg
     1681 cgccggcggg tacgtcgatc gggcgcgtca tgtcgagcag gtccggcagg tgcatccgca
     1741 gctcgagcgg ggcgaccccc aggcgttcgg gatccggcgt aggctcgtag atgcccgcca
     1801 cgcggaactc gcgcggctgg gttccggcga cctcgcttgc cagcgtgacg gtgctgccga
     1861 cggcgagtcg ctcggcttca gcgagatggc gggagatcag gatgtcgggg atggcgcgct
     1921 gctgcgccga ggccagtcca ggcgacgccg ccagcgcgag cgcgagcagg atcgatccgc
     1981 acttcacgaa cggtccgacc gactgatcgc ctcgatgcgc gcccattccg cgtcggtgac
     2041 cggcatgacc gacagcctcg acatgcggac gagcggaaac gaggcgaacg ccttgtccgc
     2101 cttgatttcc ttgagcgtca ccgggcgggt cagcttcttc tcggggacga cgtccacgac
     2161 cgacagcttc tccgatcggt cgcgtggatc cggatacgcg tggctcgcgg ccctggcgat
     2221 cgcgaccacc gccttttcct tgcccgtgtg gtagtagaag atccggtcgc cgctgcggat
     2281 ggcgcgcagg tgcttctgcg cgagcggatt cttcacgccg gcccacaccg tcttgccgtc
     2341 tttttcgagc ttgtcgtacc cgtaatgctc gggttcttct ttcacgagcc actgcgccat
     2401 gtgcggatca taacgtagtg tcccgggtcc gcctgaaggc ggacactact tcggacgtac
     2461 gtagtgtccg gcttcagccg gaccgggata ccattcgacg ctcgacctcc gcgacgcatg
     2521 ccacactcga ccacccgcca gggaatcatc gcgaccacgt ccggcgccat caccggggtt
     2581 gcggccggct tcatcggcgt cgggggcggc gagttccgga tcccggtgct cgtctcgctg
     2641 ctcggattcc cgctgaggct cgcgggcggc gtgaatctcg tcgtcggcct cttcaccgtc
     2701 gcgttgggcg tgcttcggag atgggggcag cagccgctca cgcgcgacga cctgatactg
     2761 gtcggcatca tgggcgtcgt gtctctcgcc ggcgcggcgg ccggcgtgtt gggccgcaag
     2821 aggatgcctg ttcgcccgct ccgcgttgtc ttctgcgcgt acctgatcgt cgcgggcctc
     2881 tggatgctgt acgagtcgat cgcacacgtc gagcacgtgc tgctcgagcc caccgggatg
     2941 ctgcgctgga cgctggcggc tgcgatgggt tttgccattg cgctcgtcag cggtgtgctg
     3001 ggtgtcgccg gcggcgagat gcgcattccg gcgctgctgt acctgtttgg cgtgccgatt
     3061 gtcgaggcgg gcaccctcag cctcgcggtg tcgatcccca cggtcgcggc cggcgcgttc
     3121 acggatcaca gaaccggcgg cattccaaga cccgcgatga ggcttgcgct gctcatgggc
     3181 atcgcatccg ccgtcggcgt gcttgccggc gcggcgctcg tgccctatgc cagtcgtgag
     3241 gtgatcaaag gggtgctcgg cgtcgtcctg ttgctcgcga cggtccggct ggccgtcggg
     3301 ccttcgcact gacccgcgcc cgcctcaccc ccgacgccac gagcccccgc tcgcgtaccg
     3361 caggtacatc gtcggcacca ccaccatgtt cagcagcgtc gacgtcgtca acccgcacag
     3421 aatgacgatc gccatcggcg tctgaatctc gctccccgct ttgccgccgc ccagggccaa
     3481 cgggatcagc gccaggccgg ccgccatcgc ggtcatgagg atcggaatca ggcgctcgcg
     3541 ggcgccgcgc tcgaccgctt cgcggaaatc ggtcacgcct tcgtgctccg tcagatgtcg
     3601 gatgtgcgag acgagcatga tgccgttgcg cgtggcgatc ccgaagagcg tgatgaatcc
     3661 gatcatggac gccacgctca ggacgccccc ggccaggtag acacccgcga cgccgccgat
     3721 cagcgcgagc ggcagattga gcatgaccag cagcgcatcc cgtggacgcc cgaacgccag
     3781 gacgagcagc atgaacagcc cggccacgac cgccgcgccg agcaggagca ggcgctcggc
     3841 tgcgctctgc tggctttcga actgtccgcc gtactcgaca cggtacccgg gcggcatggg
     3901 cacgtcacgg ccgatcgccg atcgcatgtc gtcgacgacg cttccgaggt cgcgtccggc
     3961 gacattcgac gagatgacga tccgacgctg cacgttctcc cgcagcacca tgttgggacc
     4021 cgtttcgcgg cgcacgtcgg ccaggagccg aatggggacc ggaccgccat tgggggtgtc
     4081 gatcggcagg tccgccaggc gatcgaagtc ggcctgtgac gccggattga acttgacgac
     4141 cagggcgacg gccgtcgcgc gatcgaagat ccgccccacg acggcgccgg ccagggcggt
     4201 ttcgacggcc tcggcgacat cgctcgccct cagcccgtag cgcgcgatgg cgggccggtt
     4261 cagcacgaag cgcacgaacg gcacgtccgt ctgctgctcg agagacacgt caaccgcccc
     4321 gggcaccgct gtcatcacgt cgcggacgcg ttcgcccagc cggcggagcg tgggcaaatc
     4381 ctcgccgaag accttgacgg cgatgttcgc gcgagtgccc gacaacatgt gatcgatccg
     4441 gtgcgagatc ggctggccga tcgtcacgtt ggtcccgggc agcgtggaga agcctcggcg
     4501 gagctccgcc agcagctcgg ctctcggccg ttcggcctcc cgcagaccga cgtcgatctc
     4561 ggcggcctct acgccctgga cgtgctcgtc gtattccgcc cgtccggtgc ggcgggcggt
     4621 cgcccggacc tcgcgctgcg ccagcaggat ctgctcgatg cggcgtccga tctcgtcgga
     4681 cttggcaagg gacgtccccg ggagggtatt ggcctgaatc gtgagcgacc cttcatggaa
     4741 ctccggcagg aaggccgtcc ccatgcgggt catggccgcc gctgccgcga acaggagcac
     4801 gaccgacgcg gccgccacga gccgcgggtg atcgagggca cgaggcagag ccagggcgaa
     4861 gcgggccttg agcacccggg ccagccagcc gtcatggcct cgcaggatcg atcgggcacc
     4921 gggcaggaag gcgcagctca gcgcgggcgt gaccacgatc gcgacgccca gcgacgccag
     4981 cagcgcgacg atgtaggcaa aggccagcgg cgaaagcagc cgtccctcga cgcccgacag
     5041 accgaacacc ggcagaaaga ccagcacgat gatgatcgtg gcgaagacga tcgacgtgcg
     5101 gatctcgagc gtggcgtcgc gcaccacatc cgcagcgggg cgctgatcgg caggcgccct
     5161 cgcggcgttc tcgcgcaggc gccggaccac gttctcgacg tcgatgatgg catcgtcgac
     5221 gagcgcgccg atggcgatcg ccatgccccc gagcgtcatg gtgttgatcg tcgcgtcgaa
     5281 gaagcgcagg accaggaccg ccgcagccag cgacagcggc atcgccgtca gggtgatcgc
     5341 cgcagcccgc atgttcgcga ggaagagcac gacgatcgcg atgacgagca ggccgccgtc
     5401 ccgcagcgcg gcgatcacgt tgccgacggc gacctcgatg aagtccgact ggcggaagat
     5461 caggcgatcg atcgtcatgc cgggcggcag ctcccgctgg agcgagtcga gctcccgatc
     5521 gagccgcgcg gtcagttcca cggtattcgc gtcgggctgc ttctgcacgc cgacaatcac
     5581 ggccggctcg ccgttccggg agccttcgcc gcgcttgagt gcggcgcctt cgcggacgtc
     5641 ggcaatatcg cgaatcagca ccgaccggtc gccgcgaagc gcgacgacgc tgtccccgat
     5701 ttcctctggc gtgcgcacgc ggccgatcgt ctggagcacg tactcctgcg gcccctccat
     5761 gtagatgccg gcggacgtgt tctggctcgc cccgcgcacg ccgtcgagca gctcgctgag
     5821 cgagatgccg ttggcgcgca gttgatccgg atgggcgatc acctggtact ggcgctcggc
     5881 gccgccgatc gacgtgactt gcgagacgcc ggctaccgcg aggagccgcc gccgcacgag
     5941 cgtgtcggcg atcgtacgaa gcgccagcgg atcctcctgc ttcgacgaga tggcgaagaa
     6001 gaggatctcg cccatgatgg acgagatcgg cgccaggatc ggcggttcga cctgcggcgg
     6061 aaggcttccg ccaaccagcg cgagcttttc ggcaacgacc tgccgtgcac ggaagatgtc
     6121 ggtcccccag tcgaactcga cccacaccac ggcgatgccg acggcggtgg cggatcggac
     6181 gcggcggacg tcggaggcgc cgttgatggc ggcttcgatc ggaaacgtga cgagggtctc
     6241 catctcttcc ggcgccatgc cgcgcccctc ggtcaggatc gtgaccgtcg gcgccgtcag
     6301 atcggggaag acgtcgaccg gcatcgtcca cgcggtccac gcgccggcgg cggcgagcag
     6361 gatcgacagc cccaggacga tccagcgatg gtcgatcgac cattgaatca gctgtttcat
     6421 gctgaccttc agtgcacgtg gccttccgcg ggcaatcctc ttgccgcgga ggcgagttga
     6481 acgtcgtagg cgccgcgcgt gaccacgcgc tcgccgggcg tgacgccgct cctgatgccc
     6541 acgagatcgc cgtcgcgcga cgcgatctcg atgaaccgcc gcgcgaaccg ctcgcccccg
     6601 gtttgcacga acacgtaggg ccgcccggct tccatcagga cagccgcagc cggcaccacg
     6661 ggcactcgcc gccgctcgcg cccgaagagc accgccgtgc ccacctggcc gatgagcagc
     6721 tgttcgccgg ggttggcgat ttccatctgc agcggcagtg cgcgggttcg cggatcgatc
     6781 acgcccgcat cgtgcacgtg ttgcggatcg agcgtcagcg gcgccgggag ccccgggatc
     6841 tccagggcga gtccggccgt ctgcctggcg gcggccacgt cggcggccgg cacctgcacc
     6901 tccagctcga cgcgatcggt cctcacgatt cggaacagcg gcgcgccctc ttcgtaggcc
     6961 gctccgagcg tggccgagac ttcggcgagc cgtccggcga tcggggcccg gagggcgaac
     7021 gcgttcccgg ccgccgcgcc tccgccggtg cggagtgttt cgtcgcgctg cgcgagacgg
     7081 gcatccgcgg ctcgcaggcg cgcttcggcg acggcgaccg cccggcgcgc atcctccacg
     7141 cggcgcgcgg gcacggcgcg gtcggccagc agccgctcgg ctcgtccctg ctcgacgcga
     7201 gccgcctcga gtcctgcgcg ggcttcggcg gcgtctgcgt ccagcgtcgc gcggtcggcg
     7261 ccagaggcga gacgtggctc gagccgtccg aggatctgat tcgggctgac gcgctccccg
     7321 atcgagagca gcgtgtcggc ctggaagcgg ccggaggcgg gagcggcaac gatcgcttcg
     7381 ccgcccggaa gcgggtggat ggtcgccggc gcgcgcaccg acgtgcgcac ctcgccttcc
     7441 cggaccaccg ccgtcgcgaa cggattcgtc cactgcggct ccttcagata cgcaatggcc
     7501 gacggatcct cggccggctg tttctccgca tcggctctgg cggtggcctc gtcggcaaag
     7561 atggtaatga ctccgaggtc gtgccgatcg gagagggccg ctccttcgag gacgagcgcc
     7621 cagcgatatc ggccgggcgc cggcggcgcc tcctcgatcc ggaacgcgcc aggccgggac
     7681 ggctcgggac cggtcaacgc gcgtggcgcg ccgccggact c
//
LOCUS       DBNGSH010000562         6809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2263348, whole genome shotgun sequence.
ACCESSION   DBNGSH010000562 DBNGSH010000000
VERSION     DBNGSH010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2263348"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="AC16CY_12960"
     CDS             complement(<1..1100)
                     /locus_tag="AC16CY_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3354749.1"
                     /translation="MPRLKTRVAATVLCATLGLAAASADDNWPRFRGAEGAGVAADHP
                     ALPDAWGPSQNVVWTIDVPGRSWSSPVVWGDHVFVTTAINTVEAESLLPASSYVARSS
                     GGPMTFKDIATSSAPHRWVVYDIDLKTGRVRWERDVHKGVPAKSRHLKNSYAAETPVT
                     DGERVYAYFGNVGLFALDMSGKPVWSKPMDAFEMLTGFGHAKSPVVDDRNVYIVNDNE
                     EASFIAAFDKRTGNQIWRTDRKETSNWTTPLVWKNERRTEIVTAATGGVRSYGVDGTL
                     LWQLTGMSNFDVPSPLAVNGLLYVMSGYTSNPLRPAYAIKPGASGDISLKPNETSNDF
                     IVWSDPTLGTFHPSPLVYRGCFYILHDRGFLT"
     gene            complement(1287..4109)
                     /locus_tag="AC16CY_12965"
     CDS             complement(1287..4109)
                     /locus_tag="AC16CY_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3354750.1"
                     /translation="MTALAAGARLGPYEIQSALGAGGMGEVYKARDSRLDRTVAIKVL
                     AASWVGSPEMKQRFDREAQTIASLNHPNICVLHDVGRQEGTDFLVMEYLEGETLASRL
                     EKGPLGVDEALKAAAAIADALDKAHRRGVVHRDLKPANVMLTSTGPKLLDFGLAKWKV
                     LPNTGSGSGSVLQTRTDLSSAGTVLGTLQYMSPEQLEGSEADARTDIFAFGALVYEMV
                     TGKRAFEGRSRVLLISAIATSHPEPLSKLQPAASPALDHVVRTCLAKDPADRWQTARD
                     LLAELQWAADAGAGNQGGLAVGSPWWRTRPGLVRIGLAAAALLVVGFTVPALLALQGR
                     GGPGETRFRVPLQLSAQADGLNIAGVAAGALFRPAGFAVSPDGRWLAFVARGNTGDAF
                     SLYVRPIGSVTPQRLAGTDDASQPFWSADSRSIGFVAGGKLKKVERTGGPPQELAPAP
                     DFSGGTWNRAGAIVFGTGQGLFRVPAEGGTPERITTVEKSETGHLWPSFLPDGRRFIY
                     TVWSGEPSGRALIAGSLDSADRRRLMPAESNAGYADAGYLVFSRGGTIYAQPFDEDGL
                     TLSGEPSRVADDVVYDTANGRSHYSLSENGVLVYFFNRNTGSTGGGSQSDMGEWQYSW
                     VNRTGQPGRSVGPWGPYRGVDVSPDTTRVAVHRHDPKGGDIWILEPRGSETNLTFDAS
                     RHNTMPVWSPDGKEIVYASQQKGKWGLYKTLSSGSGTEELLHESDLPKAPMSWSPDGK
                     RIVFWVQDPKTAGDLWVLELDGEKKAAPFIMNSPFNETHAQISPDGKWIAFTSNLRDN
                     RNEIYVRPFPTGSGQWRVSEAGGDWPRWKGDSKEIYFHSIGNNAAPSTSFGNGAFAGP
                     LYAATVSATGGVFEPGEPRQILNFPALNVTHSGGDYHNYAVSPDGERFLVPQYVQSTG
                     GAGGQLGADLLSGLTVAVNWVASIER"
     gene            complement(4106..>6809)
                     /locus_tag="AC16CY_12970"
     CDS             complement(4106..>6809)
                     /locus_tag="AC16CY_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3354751.1"
                     /translation="HICTLHDIARERPVVGATSETVRLKADTTGEAAAEDVATPEADV
                     APSVEVEELDFLVLEYVEGQTLAARLEKGPLSLRQAVEVAIAIADALDKAHQRSIVHR
                     NLTPSNVMLTADGVKLLEFGLTRPLRASAQDISATARTMAADATGVAALPDTLQYLAP
                     EQVEGKDGDARSDIFALGAILHEMVTGAKAFEGKNQTLLIAAIVSLDLDPLSKLRPGT
                     PPALDHVANRCLEKDPEARWQTAHDLGVQLRWIAEGGAVLPSTGRNLDRLVQAGVALA
                     IVLAVVTSVSAFIYARGANTPEPFQFRVPVYGITPSDIAISPDGETIAAVARPDPPEA
                     AALYVRAVREPTFRRVAGTDDASQPFWSPDSKSVAFVAGGRLKRVEAAGGPPENIAEA
                     SGITGGTWAADGTILYGSSKGVFRVSAQGGGKPEAVTAVDKGETGHYWPSFLPDGRHF
                     TYLAWSTESGGRAVFAGALGSQERRRLVAAESNALYASGYLVYHREATLFAQAFDPDR
                     LEPTGDALHIADELAFNATNGRGSFDVSQNGVLLYYQGQGGPSGRGQTQTGAQFGWRD
                     RGGGQLGVAGDPGQYGDFDLSPDAKFVAVTRQDPGAPGADIWVTEWQRGATTRLTLDP
                     ADDINPVWSPDGLRIAFTTFRKGNADIYVKNANGVGPETPLVDTPANEFVEDWSKDGK
                     YIAYRLGREAVEDIHVIPLSGDKPMTIPVVVGSFKKDEAQFSYDGKWIAYTSDESGAF
                     QVYVIDFPGLKERRQVSTTGGGQPRWDDTGKRIFYRSPDGLIMEVALELGTPVVPGVP
                     RVLVPAFLSAQSVAAERHQLSVTRDGNRFLLRYPNAGQGGFAGAAAAQGRGSGGGVAF
                     NYSPPGLAGGGPGAIGRGNAFSNAPPLGLTVIRDWTALFREVGK"
BASE COUNT         1094 a         2342 c         2217 g         1156 t
ORIGIN      
        1 cacgtcagga aaccgcgatc gtgcaggatg tagaagcagc cgcgatacac cagcggagac
       61 gggtgaaagg taccaagcgt cggatccgac cagacgatga aatcgttgct cgtttcgttc
      121 ggcttcagtg agatgtcgcc ggacgccccg ggcttgatcg cgtatgccgg gcgaagcggg
      181 ttcgacgtgt acccggacat cacatacagg aggccgttga cggccagcgg gctcggcacg
      241 tcgaagttcg acatgccggt gagctgccag agcagcgtgc cgtcaacgcc gtaggagcgg
      301 acgcccccgg tggccgcggt cacgatctcg gtgcgccgct cgttcttcca caccaggggc
      361 gtcgtccagt tgctcgtctc cttccgatcg gtgcgccaga tctggttgcc cgtccgtttg
      421 tcgaacgcgg caatgaagga cgcctcttcg ttgtcgttga cgatgtagac gttcctgtcg
      481 tccacgaccg gcgacttcgc gtggccgaac cccgtgagca tctcgaaggc gtccatcggc
      541 ttcgaccaga ccggcttgcc cgacatgtcg agcgcgaaga gcccgacgtt gccgaagtac
      601 gcgtacaccc gctctccgtc ggtgaccggc gtctcggcgg catagctgtt cttcaggtgc
      661 ctcgacttgg cgggcacacc cttgtgcacg tcgcgctccc atcgaacccg gccggtcttg
      721 agatcgatgt cgtagacgac ccagcggtga ggcgccgacg aggtcgcgat gtccttgaag
      781 gtcatcggcc cgccgctcga gcgcgccacg taggacgagg ccggcagaag cgactccgcc
      841 tcgacggtgt tgatggcggt ggtcacgaag acgtgatcgc cccagacgac gggcgagctc
      901 caggaccggc ccggcacgtc gatcgtccag acgacattct gggacggccc ccacgcgtcg
      961 ggcagcgcgg ggtggtcggc cgcgacgccg gcgccctccg ctccccggaa tctgggccag
     1021 ttgtcgtcgg ccgatgcagc cgccaatccg agcgtcgcgc aaagcaccgt ggcggcaact
     1081 cgcgtcttca gtctgggcat ggtgggcgta atcatgtgct gcgatcggag ccgcgtcaat
     1141 gccgctacac gaaggacacg aaggattcgg ccacgaaggg cacgaagatc aggaggcatt
     1201 cagacgatgt ggattcgtga ccgtcgtgtt cttcgtggct gatcggtctt cgtgttcttc
     1261 gtggctgact tcgtgatctt cgtggcttag cgttcgatgg acgcgaccca gttgaccgcg
     1321 accgtcagcc cgctcagcag atccgcgccg agctgacctc cggctccacc ggtcgactgc
     1381 acgtactgag gcacgaggaa acgttctcca tccggcgaca cggcgtagtt gtggtagtcc
     1441 ccaccgctgt gcgtcacgtt gagcgcggga aagttgagaa tctggcgcgg ctcaccgggc
     1501 tcgaacacac cgcccgtggc gctgactgtt gccgcataga gcgggcctgc gaaggcgccg
     1561 ttgccgaagc tcgtggaggg cgccgcgttg ttgcctatcg agtgaaagta gatctccttg
     1621 ctgtcgccct tccaacgcgg ccagtcgcct cccgcttctg acacccgcca ctgccccgag
     1681 ccggtgggaa acgggcgcac atagatctcg ttccggttgt cgcgcaggtt cgaggtgaac
     1741 gcgatccact tcccgtctgg cgagatctgt gcgtgcgtct cgttgaacgg ggagttcatg
     1801 atgaatggtg cggccttctt ttcgccatct aactccagca cccacaaatc gccggccgtc
     1861 ttgggatcct gcacccagaa gacgatgcgc ttgccatcgg gcgaccacga catcggagcc
     1921 ttcggcaggt cggattcgtg gagcaactcc tccgtgcccg acccgctcga gagcgtcttg
     1981 tacaagcccc acttgccctt ctgctgcgag gcgtacacga tctcttttcc gtcgggcgac
     2041 cagacgggca tcgtgttgtg gcgcgaggcg tcgaaggtga ggttggtctc cgagccgcgc
     2101 ggctcgagga tccagatgtc gccccctttc gggtcgtgcc tatgcacggc cacgcgcgtc
     2161 gtgtcgggcg acacgtcgac gccccgatag ggcccccacg gcccgacgct gcgaccgggt
     2221 tgcccggtcc ggttcaccca cgaatactgc cactcgccca tgtccgactg tgagccaccg
     2281 ccggtggaac ccgtattgcg attgaagaag tacaccagca ccccgttctc cgacagcgaa
     2341 taatggctcc gtccgttggc ggtgtcgtac acgacgtcgt ccgcgacgcg cgacggctct
     2401 ccggacagcg tgagtccgtc ctcgtcgaat ggctgggcgt agattgtgcc tcccctgctg
     2461 aacacgaggt agcccgcatc cgcgtaaccg gcgttcgatt cagccggcat caggcgcctt
     2521 ctgtccgcgg agtcgagcga acctgcaatg agggcgcgcc ccgaaggctc gcccgaccac
     2581 acggtgtaga tgaatcggcg tccatcgggc aggaagctcg gccagaggtg accggtctct
     2641 gacttctcca cggtcgtgat tcgctccggc gtgccaccct cggcaggcac gcggaacaga
     2701 ccctgcccgg tgccgaacac gattgcgcca gcccggttcc acgtgccgcc ggagaaatcg
     2761 ggcgcggggg ccagttcctg cggcggacca ccggtccgct ccaccttctt caacttcccc
     2821 ccggcaacga agccaatcga acggctgtcc gccgaccaga acggctggct ggcatcgtca
     2881 gtacccgcga gccgctgcgg cgtcacgctg ccgatcgggc ggacgtacaa ggagaaggcg
     2941 tcgcctgtgt tcccccgggc cacgaacgcg agccaacgcc cgtccggcga cacggcaaag
     3001 cctgccgggc ggaaaagcgc gccggcggcg acgccggcga tgttgaggcc gtcggcctgc
     3061 gccgaaagct ggagcggcac gcgaaaccgc gtctcgcccg ggcccccgcg tccttgaaga
     3121 gcgagcagcg cgggcacggt gaatcccacc accagaagcg cggcggccgc gaggccgatg
     3181 cggaccagtc cgggacgcgt tcgccaccac ggcgagccga ctgccagacc tccctgattg
     3241 ccggcgcccg catccgcagc ccattgaagc tccgcgagga gatcgcgtgc cgtctgccac
     3301 ctgtccgcgg gatcctttgc cagacaggtg cgcacgacgt gatcgagcgc tggagacgcg
     3361 gcgggctgga gcttcgagag cggctcgggg tgggacgtgg cgatcgccga gatcagaagc
     3421 acgcggctcc gcccctcgaa ggccctcttc cccgtgacca tctcgtagac gagcgcgccg
     3481 aacgcgaaga tgtccgtgcg ggcgtcggcc tcgcttccct cgagctgctc cggcgacatg
     3541 tactgaagcg tcccgagcac cgtgcccgcc gaggacaggt ccgtcctggt ctgcagcacg
     3601 gatcccgatc ccgagccggt gttcggcagc accttccatt tggccagtcc gaaatcgagc
     3661 agcttcgggc cggtcgacgt gagcatgacg ttggccggct tcaggtcacg gtgcacgacg
     3721 ccgcgccggt gcgccttgtc gagcgcatcg gcgatcgccg ccgccgcctt cagcgcctcg
     3781 tccacgccaa gcgggccttt ctcgaggcgc gacgccagcg tctcgccctc gaggtactcc
     3841 atcaccagga agtcggtgcc ctcctggcga ccgacgtcgt gcagcacaca gatgttggga
     3901 tggttcagcg acgcgatcgt ctgcgcctcg cggtcgaagc gctgcttcat ctcggggctg
     3961 ccgacccagg aagccgcgag gaccttgatc gccaccgtgc ggtcgaggcg agagtcgcgc
     4021 gctttgtaga cctctcccat gccgcctgcg cccaaggcgg attggatctc gtacggtccg
     4081 agcctcgcac cggcggccag cgccgtcact tgcccacctc ccgaaagagc gccgtccaat
     4141 cgcgaatcac ggtaagaccg agcggagggg cgttgctgaa ggcattgccc cggccgatcg
     4201 cgccggggcc gccccccgcc aggcccggcg ggctgtagtt gaaggcgacg cctcctcccg
     4261 agcctcgacc ctgggcggcc gccgcccccg cgaacccgcc ttgtcctgcg ttcgggtacc
     4321 ggagcagaaa gcggttcccg tcgcgcgtca cggataactg atggcgctcg gcagccacgg
     4381 attgggcgct caggaacgca gggaccaaca cgcgaggtac gccgggaacg acgggcgtcc
     4441 cgagctcgag cgccacctcc atgatcagcc cgtctggcga ccggtagaag atgcgcttcc
     4501 ccgtgtcgtc ccatcgaggc tgaccgccgc ctgtcgtgga gacctggcgc ctctctttga
     4561 gaccggggaa gtcgatcacg tacacctgga acgctcccga ttcgtcggac gtgtaggcga
     4621 tccactttcc gtcgtaggaa aactgcgcct cgtctttttt gaacgaaccc acgacgacag
     4681 gaatcgtcat gggcttatcg ccagacaggg gaatgacatg gatatcttcg acggcttccc
     4741 gccccagcct gtaagcgatg tacttgccgt ccttcgacca gtcctcgacg aactcgttcg
     4801 ccggcgtgtc gacgagtggc gtctcgggcc cgacgccgtt ggcgttcttg acgtagatgt
     4861 ccgcattgcc cttgcggaac gtcgtgaagg cgatgcgcag gccgtcggga gaccacaccg
     4921 ggttgatgtc gtcggccggg tcgagcgtga gcctcgtcgt ggcgccgcgc tgccactccg
     4981 tcacccagat gtcggcaccg ggcgcgcctg gatcctggcg ggtgacggca acgaacttcg
     5041 cgtccggcga caggtcgaag tctccgtact gcccgggatc tccggccact ccgagctggc
     5101 cgcctccccg gtctctccag ccgaactggg cgccggtctg agtctgccct ctgccggatg
     5161 gaccgccctg accctggtag tagagcagca cgccgttctg cgagacgtcg aagctgccgc
     5221 gaccattggt cgcgttgaac gccagttcgt ccgcgatgtg gagcgcgtcg ccggttggct
     5281 cgagccgatc ggggtcgaac gcctgcgcaa agagcgtcgc ctcccggtgg tacacgaggt
     5341 accccgacgc gtacagggcg ttcgattccg cagccaccag gcgccttctc tcctgcgagc
     5401 cgagcgcgcc ggcaaacacc gcacgtccgc ccgattcggt cgaccatgcc aggtacgtga
     5461 aatgccgccc atcgggcagg aaggacggcc agtagtgacc ggtctcgcct ttatcgaccg
     5521 cggtgaccgc ctccggcttt ccgcctccct gagccgacac gcggaagacg cccttcgagg
     5581 acccgtagag gatcgttccg tcggccgccc acgtgccgcc cgtgattccc gacgcctcgg
     5641 cgatgttttc cggggggccg cccgcggcct cgaccctctt cagacgcccg ccggccacga
     5701 acgcgaccga cttgctgtcc ggcgaccaga acggctggct ggcatcgtcg gtgccggcaa
     5761 cacggcggaa cgtcggctca cgcaccgcgc gcacgtagag cgccgcagcc tccggcggat
     5821 cgggcctcgc gaccgccgcg atggtttctc cgtccggcga gatggcaatg tccgacgggg
     5881 tgatgccgta caccgggacg cggaactgaa acggctccgg cgtattcgcg cctcgcgcat
     5941 agatgaacgc ggacacggac gtcacgacgg cgagcacgat cgcaagagcg acgcccgcct
     6001 ggacgagacg atcgagattc cggccggtcg agggcagcac ggcgccccct tccgcgatcc
     6061 atcggagctg cacgcccagg tcgtgcgccg tctgccagcg tgcctcggga tccttttcga
     6121 ggcaccggtt cgcgacgtga tcgagcgcgg gcggcgtgcc cggcctcagc ttcgacagcg
     6181 gatcgaggtc gagcgacaca atcgctgcga tgagcagcgt ctggttcttg ccctcgaacg
     6241 ccttcgctcc cgtgaccatc tcgtgcagga tcgcgccgag ggcgaagatg tccgagcggg
     6301 cgtcgccgtc ctttccctcg acttgctcgg gcgccaagta ctgcagcgtg tctggcagcg
     6361 ccgccacgcc cgtcgcatcc gcggccatcg tccgcgcggt tgcggagatg tcctgcgcgg
     6421 acgcacgcag cggcctcgtc aatccgaatt cgagcagctt gacgccgtct gccgtgagca
     6481 tcacgttcga cggcgtcaga ttgcgatgga cgatgctcct ctgatgcgcc ttgtccagtg
     6541 cgtcggcgat cgcgatggcg acctcgactg cctgacgcag cgacagcgga cccttctcga
     6601 gtcgtgcggc gagcgtttgt ccttccacgt actcgaggac gagaaagtcg agctcctcga
     6661 cctcgacgga gggagcaacg tccgcttcgg gagtagccac gtcctccgcg gcggcctcac
     6721 ccgtagtgtc cgccttcagg cggaccgtct ccgacgtcgc gccgaccacc ggacgttcgc
     6781 gggcgatgtc gtgcagcgtg cagatgtgc
//
LOCUS       DBNGSH010000563         2890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1584286, whole genome shotgun sequence.
ACCESSION   DBNGSH010000563 DBNGSH010000000
VERSION     DBNGSH010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1584286"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..2246)
                     /locus_tag="AC16CY_12975"
     CDS             complement(222..2246)
                     /locus_tag="AC16CY_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3354752.1"
                     /translation="MAFIRRLMFVMLLVAAVSWPFIGRGQTGAKNNEWRAYRGEEAST
                     GYSPVDQINRDNIKTLEVAWTWRFDNYGTAAQTATTETTPLMVGGVLYFTAGQRRTVV
                     AADAGTGETLWTWRLDEGARFDAAPRKVHRGVAYWTNGQEERIVLVTPGFQLVALNAK
                     TGRPVQGFGQGGIVDLFKELDLDVPLDPMGRIGNSSPVVISNDVIVVGPALQPGGRVN
                     ISNVKGDVMAFDVRTGRKLWTFHTIPREGEKGSETWLGDSAQYTGNAGVWGPSSADPQ
                     LGYVYLNIESATNDVYGGHRPGNNLFSDSIVCLDIKTGKMIWYQQLVHHDIWDYDLPP
                     APILIDLTVDGKPVRAVVQLSKQAIAYVFDRTNGQPVFGWTERKVPQTDVPKEWTSPT
                     QPFPNKPPPFDQHGITIDDLIDFTPALRAEAVKAIEQFKIGPIYTPGTVVVEGKHRGT
                     IVVPGLGGGANWPGGAADPETGFVYVGSATNPGVIGLTPNAEPKTSGVDTDYTFGGGL
                     PTVQGLRLMKPPYGRITAYNMNRGEIAWQIPNGDTPPAVKNNPALKGLTIPKTGSGSQ
                     AGLLVTKTLLFAGEGSGGQAFFHAYDKATGAEIWQTAIPGPQTSLPMTYLHQGRQYVV
                     VGTRGPTGSGAQLVAFALPREDQGGRGGRGGRGGAPGGGGRGGGRGGDAN"
     gene            complement(2354..>2890)
                     /locus_tag="AC16CY_12980"
     CDS             complement(2354..>2890)
                     /locus_tag="AC16CY_12980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3354753.1"
                     /translation="HKETRETQIYALVLARDDGRLGPRLKRTTVDCAAIKAERRAAPG
                     QGSPAVPAGPPRLDEPPECGTRMMGRPDPNGGGINLWLGLGGTTMSNLAGLVTSYAGR
                     IVVDKTGLAGEFDLVLQFAPPGGLSTNPAGNAVPVAVEESPSVFTALQEQLGLKLESQ
                     RGPVEYLVIDSVEQPTPD"
BASE COUNT          439 a          990 c          923 g          538 t
ORIGIN      
        1 ggcccggtgg ccctcccggt cttcaaaacc ggactgctcc ctgctttgca gggaggctgg
       61 gttcgactcc caggcgcttc cgccaacctt cgctcgctac gcgagcttcg gttggcaagc
      121 caatgaacgg cttgcccgcc atagcgcgca gcgcgccggc gggtggcaag ccacgcgaag
      181 gttgcccgcc gaagccttgc aggcgaaggc gggccgcgcc ttcagttcgc gtctcctccg
      241 cgcccgcctc cgcggccgcc gccgcccggt gctccgccgc ggccgcctcg cccgccacgg
      301 ccaccctgat cttcacgcgg cagcgcgaac gcgacgagct gcgcaccaga gcccgtcggg
      361 ccgcgcgtgc caaccacgac gtactgccgg ccctggtgaa ggtacgtcat gggcaggctc
      421 gtctgcggtc ccggaatcgc cgtctgccag atttcggcgc cggtcgcctt gtcgtacgcg
      481 tggaagaacg cctgtccgcc ggagccttcg cccgcgaaga gcagcgtctt ggtgacgagc
      541 agcccggcct gcgacccact gcccgtcttc gggatcgtca gccccttgag cgccgggttg
      601 ttcttgaccg cgggcggcgt gtcgccgttc gggatctgcc acgcgatctc gccgcggttc
      661 atgttgtacg ccgtgatgcg gccgtatggc ggcttcatca gccgaagccc ctgaaccgtc
      721 ggcagcccgc cgccgaaggt gtagtcggtg tcaaccccgc tcgtcttcgg ttcggcgttc
      781 ggcgtcagtc cgatcactcc cggattggtt gccgagccga cgtacacgaa acccgtctcg
      841 ggatccgcgg cgccgcctgg ccagttggcg ccaccgccga gacccggcac tacgatggtg
      901 cccctgtgct tcccttccac gaccacggtc cccggcgtgt agatcggtcc aatcttgaac
      961 tgctcgatcg ccttgacggc ttcggcccgg agcgcgggcg tgaagtcgat cagatcgtcg
     1021 atcgtgatgc cgtgctggtc gaacggcggt ggcttgttcg gaaacggctg tgtcggggag
     1081 gtccactcct tcgggacgtc ggtctgcggc actttccttt cggtccagcc gaacacgggc
     1141 tggccgttcg tgcgatcgaa gacgtatgcg atcgcctgct tgctcagctg cacaacggcg
     1201 cggaccggct tgccgtccac cgtgaggtcg atcaggatcg gggcgggcgg cagatcgtag
     1261 tcccagatat cgtggtgcac gagctgctgg taccagatca tcttgccggt cttgatgtcc
     1321 aggcagacga tggagtccga gaagaggttg ttgcctggac ggtgaccgcc gtagacgtcg
     1381 ttggtcgccg attcgatgtt caggtacacg tagccaagct gcgggtcagc cgaggagggg
     1441 ccccacacgc cggcgtttcc cgtgtactgc gccgagtcgc ccagccatgt ctcggatcct
     1501 ttttcaccct cacgcgggat cgtatggaaa gtccacagct ttcgcccggt gcggacgtcg
     1561 aacgccatca cgtctccctt gacgttcgag atgttcacgc gcccgcccgg ctgcagggcc
     1621 ggacccacca cgatcacgtc gttcgagatc acaacgggcg agctgttgcc gatgcggccc
     1681 atcggatcca gcggtacgtc gaggtcgagc tccttgaaca gatcgacgat gcccccttgc
     1741 ccgaagccct gaaccggcct gcccgtcttc gcgttcaagg cgaccagctg gaatcccggc
     1801 gtgacgagga cgatgcgctc ctcctggccg ttggtccagt aggcgacacc gcggtgcacc
     1861 ttgcgcgggg ccgcatcgaa cctcgcgccc tcgtcgagcc gccaggtcca gagcgtctca
     1921 ccggtgccgg catcggcggc cacgaccgtg cggcgctgcc cggccgtgaa atagagcacg
     1981 ccgccaacca tcagcggcgt cgtctcggtg gtcgccgtct gcgccgctgt tccgtagttg
     2041 tcgaacctcc acgtccaggc aacctcgagg gtcttgatgt tgtccctgtt gatctggtcg
     2101 acgggcgagt acccggtact cgcctcttct ccgcggtagg cgcgccactc gttgttcttg
     2161 gcgccggtct gcccgcgacc gatgaatggc cacgatacgg ccgccacaag caacatgacg
     2221 aacatgagcc gacgtatgaa tgccatcgag gactccttcg gtgcgggatg ttgtgcttat
     2281 cagtggcgct ctgtcaattg aaaccgatag cgctggagac cggagactgt agaccgtagc
     2341 ccggagaccg tgctcagtcc ggtgtcggtt gttccacgct gtcgatcacc aggtactcaa
     2401 ccggcccccg ctgagactcg agcttgagcc caagctgctc ctgaagcgcg gtaaagaccg
     2461 agggactttc ttcgacagcg accgggacgg cgttgccggc cgggttcgtg gatagcccac
     2521 cgggcggagc aaattgcagt acaaggtcga actcccctgc cagaccagtc ttgtccacga
     2581 cgatgcgacc ggcgtaggac gtcaccaacc ccgcgaggtt cgacatcgtc gttccaccta
     2641 aacccagcca aaggttgatt cctccgccat tggggtccgg acgtcccatc attcgggtgc
     2701 cgcactcggg cggctcatcg agcctcggcg ggcccgccgg gacggctgga gaaccctggc
     2761 cgggtgccgc ccgccgctcc gccttgattg ccgcgcagtc caccgtcgtg cgtttcaacc
     2821 tggggccaag tctgccgtcg tcgcgggcaa ggacgagcgc gtagatctgc gtctcgcgag
     2881 tttccttgtg
//
LOCUS       DBNGSH010000564         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3112875, whole genome shotgun sequence.
ACCESSION   DBNGSH010000564 DBNGSH010000000
VERSION     DBNGSH010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3112875"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..>1992
                     /locus_tag="AC16CY_12985"
     CDS             255..>1992
                     /locus_tag="AC16CY_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354754.1"
                     /translation="MTDRSPRSTRWFGVRAIEDIARDVRHSIRGFGRNPGFALAVALT
                     LALGIGGNTAIFSVVDQVLLRTLPYPDGDRLVRVYESRLDRPGIRYSNGVSPANWLDW
                     QRQSRTLGSIGIWFNVTQTLTGLGEPERINAQAVSWEFFRALGVTPLLGRGLSPEDDG
                     PNAPPVAVISHRLWQRLFGGDTAVIGRKIQLNDVAVEVVGVMPPSFRFIYQDTDLWGP
                     FRLDRNLPWRQVAGRFIDVVARLAPGATLSSAEAEMRSIADTLAATYPFNKNTSVALV
                     PLREELTGQVRSSLIVLYLAVGVLLSIACFNVANLLLARAASRRHEIAIRMSLGAGRA
                     PIVRQLLVESVLLAVAGGLVGLLLARWSLDALLALAPASLLRVPELYVDQRVLLYALS
                     VSVLTGGIVGLVPALSVARRSLVARIHESGHRVSHSPWLRQSLVVAQVAMTIVLMSAA
                     GLLVRTVIALNSTNNGLDKTNLLTMAVSLPNSRYADQRATEFISRAVESLRSLPGVES
                     AGAANSPPVVGSVRGGTGFHRLGTPLPPPQEMPSTLVRVVAPGYFRTLRIPIVRGREF
                     DDSDQTAGAAPGF"
BASE COUNT          300 a          675 c          662 g          355 t
ORIGIN      
        1 cctacacgaa gccgtggacc gtgacgcagc cgctcgcctt tcaggccgac acggacctgc
       61 tggagtacat gtgcaacgag aacaacaagt acttcgacat cattcccaag gcagagggca
      121 ggggaagtaa gtaaaggggg cagggggcag ggggcagggg gcagggggta gggggcaggg
      181 ggcagccccg ccgccctcga gttcatcgtc ccaactctcc agagcgcatt cacgtctaag
      241 gcggcagata gccgatgaca gaccgctcgc cgcgctccac gcgctggttc ggcgttcgcg
      301 ccatcgagga tatcgcgcga gatgtgagac acagcatccg cgggttcggt cgaaacccgg
      361 ggtttgcgct cgccgtcgcg ctgacgctgg cgcttggcat cggcggcaac acggccatct
      421 tctccgtcgt cgatcaggtc ctgctgcgca cgttgcccta tcctgacggc gatcggctcg
      481 tgagagtcta cgagtccagg ctcgacaggc ccggcattcg gtactcgaac ggcgtatcgc
      541 cggccaactg gctcgactgg cagcgtcaga gccgcaccct cggctcgatc gggatctggt
      601 tcaacgtcac ccagacgctg acgggcctgg gcgagccgga acggataaac gctcaggcag
      661 tctcctggga gttcttccgc gcgctcggcg tgacgccgct cctcgggcgc gggctctcgc
      721 ctgaagacga cgggcccaac gcccctccag tcgcggtgat cagccaccgg ctgtggcagc
      781 gtctctttgg cggcgatacc gccgtgatcg gccgcaagat tcagctgaac gacgtcgccg
      841 tcgaagtggt cggcgtgatg ccgccatcgt tccgcttcat ctaccaggac acggatctgt
      901 gggggccgtt ccgtctcgat cgaaatcttc cgtggcggca ggtcgccggg cggttcatcg
      961 acgtcgtcgc ccggctggcc cccggggcga cgctctcgtc ggctgaggcc gagatgcgat
     1021 cgatcgcgga cacgctcgcg gcgacgtacc cgttcaacaa gaacacgtcg gtcgccctgg
     1081 tgccgcttcg ggaggagctc accggccagg tgcggagctc gctcatcgtc ctgtacctcg
     1141 ctgtcggcgt cctgctgtcg atcgcctgct tcaacgtcgc caaccttctt ctggcccggg
     1201 cggcatcgcg gcgacacgag atcgccatcc gcatgtcgct cggcgccgga cgtgcgccga
     1261 tcgtccggca gcttcttgtc gaaagcgtgc ttctggctgt cgcaggcggc ctcgtcggtc
     1321 tcctcctggc gcggtggagt ctcgacgcgc tgctcgcgct cgcgccggcg agcctgcttc
     1381 gcgtcccgga gttgtacgtg gaccagcggg ttctcctcta cgctctcagc gtctccgttc
     1441 tgaccggagg catcgtcggc ctcgttccgg ccctgtcggt ggcgcgccgc tcgctggtgg
     1501 ctcgcattca cgagagtggg caccgagtct cacattcgcc gtggctgcgt cagtctctcg
     1561 tcgttgccca ggtggcgatg acgatcgtcc tcatgtcggc cgccgggctg ctcgttcgaa
     1621 cggtgatcgc actcaacagc accaataacg ggctcgacaa gaccaacctg ctgacgatgg
     1681 cggtgtcact gccgaactca cgatacgcgg accagcgtgc gacggaattc atcagccggg
     1741 ccgtcgaatc gctgcgttcg ctgccgggcg tcgaatcggc gggcgccgcc aacagcccgc
     1801 ctgtcgtggg ctccgttcgg ggaggcacgg gctttcatcg gctcggcaca ccgctgccgc
     1861 cgccgcagga gatgccatcg acgctcgtgc gcgtcgtcgc ccccgggtac ttccggacgc
     1921 tgaggatccc gatcgtgcgc ggccgcgagt tcgacgacag cgaccagaca gcgggcgcag
     1981 cgcctgggtt ca
//
LOCUS       DBNGSH010000565         6318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1584291, whole genome shotgun sequence.
ACCESSION   DBNGSH010000565 DBNGSH010000000
VERSION     DBNGSH010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1584291"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="AC16CY_12990"
     CDS             complement(<1..326)
                     /locus_tag="AC16CY_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR3354755.1"
                     /translation="MTPVRSVRLQPDPRFADVHHAIVSCERCPRLREYCRRVAEEKRR
                     AYRDEQYWGRPVPGFGDPAARLLLVGLAPAAHGANRTGRVFTGDGIGGSGDFLMSALH
                     RTGFAN"
     gene            366..3044
                     /gene="aceE"
                     /locus_tag="AC16CY_12995"
     CDS             366..3044
                     /gene="aceE"
                     /locus_tag="AC16CY_12995"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR3354756.1"
                     /translation="MRNTAPKDAAEVEALETREWLESLDYVLQSGGPARVGRLLAALG
                     DHARRNGVAQPFTANTPYINTIPFTDQPPFPGSREIERRIKSLVRWNALAMVVKANKL
                     EDGIGGHISTYASAATLYEVGFNHFFKGKGDNHDGDIIYFQGHAAPGIYARAFLEGRL
                     SAEKLANFRRELKEGGGLSSYPHPWLMPEFWEFPTVSMGLGPIKAIYQARFMRYLEDR
                     GLKKRSDSKVWAFVGDGETDEPESLGAITLASRERLDNLIFVVNCNLQRLDGPVRGNG
                     QIIQELEAIFRGAGWNVIKVIWGCEWDALLEKDQDGLLVKRMGEVVDGEYQKYAVESG
                     EYVRRHFWGADPRLLEMVAHLSDEQLKKLSLGGHDPTKVHAAYKAAVEHTGQPTVILA
                     RTIKGYGIGEAGEGKNITHQQKKLNEDELRAFRTRFGIPIGDEDVAAAPFYRPPDNSP
                     EVSYMVERRKQLGGFVPSRKVQVEPLKTDTLPELFAEFHQGSEGRKASTTMAFVRMLA
                     KLLRDKEIGELVVPIVPDEARTFGMEALFRQVGIYSHVGQLYEPVDMDTLLYYKEAKE
                     GQILEEGITEAGSISSFIAAGSAYATHGINTIPFFIYYSMFGMQRVGDLVWAAADTRT
                     RGFLLGGTAGRTTLAGEGLQHQDGHSHLLSLAVPNLISYDPAFAFEIAVIIEDGIRRM
                     YVNGESIFYYITVMNEQYEMLPMPPGSREGILKGLYLLRPSQKKSARRAQLLGSGAIL
                     NEALKAQQVLDEKYDVAADVWSVTSYQELYRDGHAAERWNRMHPGETARVPYVARCLA
                     GTDGCIVAASDYVKALPDAIDRWTGRHVTTLGTDGFGRSEGRAALRDFFEVDARHIVL
                     ATLNDLAREGKIEAAVARKAVKDLGINPEKVNPATA"
     gene            3121..4518
                     /locus_tag="AC16CY_13000"
     CDS             3121..4518
                     /locus_tag="AC16CY_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR3354757.1"
                     /translation="MTEFKIPELGENVASGDVTRVLVNVGDTVALEQPVLELETDKAT
                     IEVPSSVAGVVRDIKVKKGDKVKVGAVVLTVDGGSTAPAPQPKAAAKAADPPQAAAPA
                     PAGPPRVVPMPARPAPEASPPPAAPAVKSNGGRSAGPAAAASPAVRRLAREIGVDVNE
                     VQGTGPDGRISLEDVKEHARRILSSVPSTLLGASGAGGAAAGGAAARATRPLPDFQRW
                     GEIERQPWTNIRRSTAERLSHAWTTIPHVTQFDKADVSALEKLRKEYKDQVAAAGGNL
                     TITAMLVMVLATAVKKFPQFNASLDGERGEIVYKKYVNVGVAVDTEHGLLVPVIRNAD
                     SKNLTEIAVELHKAAQRARDRKLSLEDMSGGGITISNLGGIGGTYFTPIVNWPEVAIL
                     GVSRAAIEPVWRPSTSSGRPELDEGREDAWRPRLTLPLALSYDHRVIDGADAMRFLRW
                     VVDAIEQPFLLSLVG"
     gene            4518..5930
                     /gene="lpdA"
                     /locus_tag="AC16CY_13005"
     CDS             4518..5930
                     /gene="lpdA"
                     /locus_tag="AC16CY_13005"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3354758.1"
                     /translation="MADSVQVAVLGAGPGGYAAAFYAADLGMQVALIDEEKNPGGVCL
                     YRGCIPSKALLHVAKVIDEARHAANWGVSFGEPSVDVNKLRSFKQGVVDKLTSGVGSV
                     AKLRKVKYLQGRATLTGPASLRVAGEAGSTDLQFQHLILATGSHPTRIPSLALESARM
                     MDSTSGLEIPDVPGSLLVVGGGYIGLELGSVYATLGSKVSVVEMTSGLLPGADRDLVG
                     VLEKRLKKLFASIMLNTKVVKVAEEGRGIRVTFEGEAAEKEQTFDRVLVAVGRRPNSK
                     IPGLETTRVKVDAKGFIETDGQRRTAEPTIFAIGDVAGEPMLAHKASHEARVAVEAIA
                     GHKAVFEPQAIPAVVFTDPEIAWCGLTEAQAQATGTEFEVAKFPWGASGRAITVDRPE
                     GLTKLLVEPKTGRILGVGIAGSGAGELIAEGVLAIEMGATAEDLKLVIHPHPTLSETV
                     MESAEVFFGQSTHVYRPRRK"
     gene            5933..>6318
                     /locus_tag="AC16CY_13010"
     CDS             5933..>6318
                     /locus_tag="AC16CY_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354759.1"
                     /translation="MRRSHFSFLGSRFVFSFGVRVLVLGSGFGVLGSLAAAQAPAAVE
                     KHIAAARAAAGTEHAGMVTRLCPASAPAATPAARGGGARRGGGPPGPPPRESWHAEPV
                     KVFDNFYFVGMTEYSTWAITTSQGIIV"
BASE COUNT         1118 a         2061 c         2195 g          944 t
ORIGIN      
        1 atgttcgcga agccggtccg gtgcagcgcg gacatcagaa aatcgcccga cccgccgata
       61 ccatcgccgg tgaagacgcg gcccgtacgg ttggcgccgt gcgctgccgg cgcgagaccg
      121 acgagcagca gacgcgccgc cggatccccg aagccgggga cgggacggcc ccagtactgc
      181 tcgtctcggt atgcccgccg tttctcctcg gcgactcgac ggcagtactc gcgaaggcgc
      241 gggcatcgct cgcatgacac gattgcatga tgaacatcag cgaaacgcgg gtccggctga
      301 agccggacac tacgtacggg tgtcatgaga gcgcagcgga gactacaata gagtctagag
      361 gatcgatgcg aaacaccgca cccaaggacg cggccgaagt cgaagcgctc gagacgcgcg
      421 agtggctcga gtcgctcgac tacgtgctcc agtccggagg cccggcccgc gtcggccgcc
      481 tgctggcggc gctcggcgat cacgcccggc gcaacggcgt cgcgcagccc ttcaccgcga
      541 acacgcccta catcaacacg attccgttca ccgaccagcc tccgtttcca ggcagccgcg
      601 agatcgagcg ccgcatcaag agcctcgtgc gctggaacgc gctcgcgatg gtcgtcaagg
      661 cgaacaagct cgaggacggc atcggcgggc acatttccac ctacgcgtcg gcggccacgc
      721 tgtacgaggt cggcttcaac cacttcttca agggcaaggg cgacaatcac gacggcgaca
      781 tcatctactt ccaggggcac gccgcgccgg gcatctacgc gcgcgcgttt ctcgaaggcc
      841 gcctgtcggc ggagaaactc gcgaacttca ggcgcgagct gaaggaaggg ggcggcctct
      901 cgtcctatcc gcacccctgg ctcatgccgg agttctggga gttccccacc gtgtcgatgg
      961 ggctggggcc catcaaggcg atttaccagg cgcgcttcat gcgctacctc gaggaccgcg
     1021 ggctcaagaa gcggtcggac tcgaaggtgt gggcctttgt cggcgacggc gaaaccgacg
     1081 agcccgagtc gctcggcgcc atcacgctcg catcccgcga gcgcctcgac aacctgatct
     1141 tcgtcgtcaa ctgcaacctg cagcggctcg acggcccggt gcgcggcaac gggcagatca
     1201 tccaggagct cgaggcgatc ttccgcggcg cggggtggaa cgtcatcaag gtgatctggg
     1261 gatgcgagtg ggacgcgctc ctcgaaaagg accaggacgg cctgctcgtc aagcgcatgg
     1321 gcgaggtcgt ggacggcgag taccagaagt acgccgtcga gtccggcgag tacgtccgga
     1381 ggcatttctg gggcgcggac ccgcggctgc tcgagatggt ggcgcacctg tcggacgagc
     1441 agctcaagaa gctgtcgctc gggggccacg acccgaccaa ggtgcacgcg gcctacaagg
     1501 ccgcagtcga gcacacggga caacccacag tgattctggc ccgcaccatc aagggctacg
     1561 ggatcggcga ggcgggtgag ggcaagaaca tcacccacca gcagaagaag ctgaacgagg
     1621 acgagctcag ggcgttccgc acgcgcttcg ggatcccgat cggagacgag gacgtggcgg
     1681 ccgccccctt ctacaggccg cccgacaaca gccccgaagt cagctacatg gtcgagcggc
     1741 gcaagcagct cggcggcttt gtcccctccc gcaaggtcca ggtcgaaccc ctcaagacgg
     1801 acacgctccc cgagctcttc gccgagttcc atcaaggcag cgagggccgc aaggcctcga
     1861 cgacgatggc gttcgtgcgg atgctggcaa agctccttcg cgacaaggag attggcgagc
     1921 tggtcgtccc gatcgttccc gacgaggcgc ggacgttcgg gatggaggcg ctgttcaggc
     1981 aggtcggcat ctactcgcac gtcggccagc tctacgagcc ggtcgacatg gacacgctgc
     2041 tgtactacaa ggaagccaag gaagggcaga tcctcgagga agggatcacg gaagcggggt
     2101 cgatctcgtc gttcatcgcg gccgggagcg cgtacgcgac gcacggtatc aacacgatcc
     2161 cgttcttcat ctactactcg atgttcggga tgcagcgcgt cggcgatctc gtgtgggccg
     2221 cggccgacac gcgcacgcgc ggattcctgc ttggcggaac ggccggccgg accacgctcg
     2281 cgggcgaggg gctgcagcac caggacggcc acagccatct gctgtcgctg gccgtcccga
     2341 atctgatctc gtacgacccg gcgttcgcct tcgagatcgc cgtcatcatc gaggacggga
     2401 tccgccggat gtacgtgaac ggcgagagca tcttctacta catcaccgtc atgaacgagc
     2461 agtacgagat gctgccgatg ccgccaggct cgcgcgaagg catcctcaag gggctctatc
     2521 tgctgcgacc ttcgcagaag aagagcgcca ggcgcgcgca gctgctcggg agcggcgcga
     2581 ttctcaacga ggcgctcaag gcgcagcagg tcctcgacga gaagtacgac gtggctgccg
     2641 acgtctggag cgtgacgagc tatcaggagc tctatcgcga cgggcacgcg gccgagcggt
     2701 ggaaccggat gcaccccggc gagaccgcgc gggtgccgta cgtcgccagg tgcctcgccg
     2761 gcaccgacgg ctgcatcgtc gcggccagcg attacgtgaa agcgctcccg gatgcgatcg
     2821 accggtggac cgggcggcac gtgaccacgc tcgggaccga cggcttcggg cgcagcgagg
     2881 ggcgggctgc gctccgggac ttcttcgagg tggacgcccg gcacatcgtg ctggccacgc
     2941 tgaacgatct ggcgcgcgag ggaaagatcg aggccgccgt tgcccgcaaa gcggtcaagg
     3001 accttgggat taacccggag aaagtgaatc cggcaacggc gtaggccccg gggcccaggc
     3061 ctcgggcctc agacagccct gaggcctgag gcctgattcc cgaggcctgc gaggagcagt
     3121 gtgaccgagt tcaagattcc cgagctggga gagaacgtcg cctccggtga cgtgacgcgc
     3181 gtgctcgtca acgtgggcga caccgttgcc ctcgagcagc cggtactcga gctcgagacc
     3241 gacaaggcca cgatcgaggt tccttcgtcg gtggccgggg tcgtccgcga catcaaggtc
     3301 aagaaaggcg acaaggtcaa ggtcggcgcc gtcgtgctga cggttgacgg aggctcgacc
     3361 gctcccgcgc ctcaacccaa ggctgcggcg aaagccgcgg atccgccgca ggccgccgct
     3421 cccgcgccag cggggccacc gcgcgtcgtg ccgatgccgg cgaggccggc gcccgaggcc
     3481 tcgccgccgc cggccgctcc cgccgtgaag tcgaacggcg ggcgctcggc cgggcccgcg
     3541 gccgcagcct cgcccgccgt caggcgcctt gcccgcgaga tcggcgtgga cgtaaacgaa
     3601 gtgcagggaa caggtccgga cggacggatc tcgctcgagg acgtcaagga gcacgcgcgg
     3661 cgcattctga gcagcgtccc ctcgacactg ctcggggcaa gtggcgccgg cggggccgcg
     3721 gccggcggcg cggccgcgcg agccacgcgg ccgcttcccg acttccagag gtggggagag
     3781 atcgagcggc agccgtggac caacatccgg cgttcgacgg ccgagcgcct cagccacgcc
     3841 tggacgacga tcccgcacgt cacgcagttc gacaaggcgg atgtaagcgc gctcgaaaag
     3901 ctccggaagg aatacaagga ccaggtcgcg gcggcgggcg gcaacctgac gatcacggcg
     3961 atgctggtga tggtcctggc gaccgccgtg aagaagttcc cgcagttcaa cgcctctctc
     4021 gacggcgaac ggggcgagat cgtctacaag aagtacgtca acgtgggcgt cgccgtggac
     4081 accgagcacg gccttctcgt cccggtgatc cgcaacgcgg acagcaagaa cctcaccgag
     4141 attgccgtgg agctccacaa ggcggcgcag cgcgcccgcg atcgcaagct ctcgctcgag
     4201 gacatgagcg gcggcggcat cacgatctcc aatctcggcg gcatcggggg cacctacttc
     4261 acgccgatcg tgaactggcc cgaagtggcg atcctcggcg tctcgcgggc ggcgatcgag
     4321 cccgtctggc gcccttcgac aagctcaggg cgtcccgagc ttgacgaggg acgcgaggac
     4381 gcgtggcggc cccgtctgac gctgccgctg gcgctgtcct acgatcatcg cgtgatcgac
     4441 ggcgcggatg cgatgcgctt cctccggtgg gtcgttgacg cgatcgagca acccttcctt
     4501 ctttcactgg ttggctgatg gcggattcgg tacaagtagc agtcctcggc gcgggtcccg
     4561 gcgggtacgc agccgcgttc tacgcggccg acctcggcat gcaggtcgcg ctcatcgacg
     4621 aagagaagaa tccgggcggc gtctgcctct accggggatg cattccctcg aaggcgctcc
     4681 tccacgtcgc gaaagtaatc gacgaagcgc gccatgcggc caactggggg gtcagcttcg
     4741 gcgagccctc ggttgacgtg aacaagctcc gatcgttcaa gcagggcgtg gtggacaagc
     4801 tcacgtccgg cgtgggcagc gtcgcgaaac tgcggaaggt gaagtacctg cagggccgcg
     4861 cgacgctgac cggcccggca tcgctcaggg tggcgggcga ggccggctcg accgatctgc
     4921 agttccagca cctcatcctg gcgaccggtt cgcacccgac gcggataccg tcgctcgcgc
     4981 tcgagtctgc ccgcatgatg gactcgacgt ccggcctcga gattcccgac gttcccggct
     5041 cgctgctcgt cgtcggcggc ggctacatcg gcctggagct cggatcggtg tatgcaacgc
     5101 tcggcagcaa ggtgtccgtc gtcgagatga cgtcgggcct gctgcccggg gccgatcgcg
     5161 atctcgtcgg cgttctcgag aaacggctga agaagctctt cgcctcgatc atgctcaaca
     5221 cgaaagtcgt gaaggtcgcc gaggagggac gcggcattcg cgtgaccttc gagggcgagg
     5281 cggccgagaa agagcagacc ttcgaccgcg tgctcgtcgc cgtgggacga cggccgaact
     5341 cgaagattcc gggcctcgag acgacgcgcg tgaaggtgga cgcgaagggc ttcatcgaga
     5401 ccgacggaca gcgcaggacc gcggaaccca cgattttcgc gatcggcgac gtggccggtg
     5461 agccgatgct ggcgcacaag gcgtcgcacg aggcgcgcgt ggccgtcgag gcgattgcgg
     5521 gtcacaaggc cgtgttcgag ccgcaggcga tcccggccgt cgtgttcacc gatccggaaa
     5581 tcgcctggtg cggcctcacc gaagcgcagg cgcaggcgac gggaaccgaa ttcgaagtgg
     5641 ccaagttccc gtggggggcg tcgggccgcg cgattacggt cgatcggccg gaggggttga
     5701 ccaagctcct ggtcgagccg aagaccgggc gcattctcgg cgtgggcatt gccggatccg
     5761 gagcggggga gctcatcgcc gaaggcgtgc tggccatcga gatgggcgcc acggcagaag
     5821 acctcaagct cgtcatccat ccgcacccga cgctgtcgga gacggtcatg gagagcgccg
     5881 aggtgttctt cgggcaaagc acgcacgtct acagaccgag gaggaagtag agatgcgtcg
     5941 ttcacacttc tcgtttctcg gttctcggtt cgtgttcagt ttcggggttc gtgttctcgt
     6001 actcggttcg gggttcggtg ttctgggttc gctcgcggcg gcgcaggcgc ccgctgcagt
     6061 ggagaagcac atcgcggcgg ccagggccgc ggcagggacc gagcatgcgg gcatggtcac
     6121 gaggctgtgt cccgcgtctg cgcctgctgc gacgccggcc gcccgcggtg gcggtgcccg
     6181 gcgcggcggc ggaccgccgg ggcctccccc tcgggagtcg tggcacgccg aaccggtcaa
     6241 ggtcttcgac aacttctatt tcgtcggcat gacggagtac tccacgtggg ccatcacgac
     6301 ctcgcagggc atcatcgt
//
LOCUS       DBNGSH010000566         2589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4300711, whole genome shotgun sequence.
ACCESSION   DBNGSH010000566 DBNGSH010000000
VERSION     DBNGSH010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4300711"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="AC16CY_13015"
     CDS             <1..776
                     /locus_tag="AC16CY_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR3354760.1"
                     /translation="AALSLASMALLAQAPMTNLGYTDTPMLPGAPYRVHDPARPHPVV
                     VTPGAQAGAPPSDAIVLFDGRDLSRWTAAKQAWKVENGYVEVVPDSGDMRTKDTFGDS
                     QLHVEWAAPAEVRGSSQNRGNSGIFLQGRYEVQVLDSFDNPTYADGQAGAIYGQWPPL
                     VNPVRKPGEWQSFDIVFEAPRLDGARLVTPAYLTVFLNGVLLHHRREVMGPTVHRALA
                     KYAAQPSEDALVLQDHQQPVRYRNIWIRRLRGYDSAAPR"
     gene            complement(773..2284)
                     /locus_tag="AC16CY_13020"
     CDS             complement(773..2284)
                     /locus_tag="AC16CY_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3354761.1"
                     /translation="MRCSSVRRARLGLAALVLASVAAQPASAVSWTRLRSQNFLFVGD
                     APEGAIHRVARSLEQFRNVMALVLPGASDSSAVPPVVMVFSSDRAFTPFKPRYKGRPI
                     ENVGGYMHLSDDVTYIAMNVERSRRAVFHEYTHFLIANTIGVVPPWLNEGLAEFYQMY
                     EDDGGRAAIIRPSFSHLRLLQRGPLMPLSELLAVEQDSPHYNEGDRRGIFYAQSWALV
                     HYLAVGSASRSGQLSDYLDRVERGTPLDEAFRGAFKTEPGVLEQELRNYLRNLPLRTL
                     RLNFPEKLEAAPVARGDAIAEPEAQGYLGDLLARVTPDAGRAYLQTLVSANGQNARAS
                     ASLGLLDLREGRIDDALARLERAVAIEPQNGAFQGALGRALAEQLQGKQKDLDMVVAI
                     LARARAVLTRAAELEPSSAHVLATLGYIEYVDGADLVRAAALLEHAIRLAPARDPYRL
                     MLAEVLVQQKRYDRAVSVLRALLARGDDEAIRAEAQALLGRMGTKDQAPSTKD"
     gene            complement(2298..>2589)
                     /locus_tag="AC16CY_13025"
     CDS             complement(2298..>2589)
                     /locus_tag="AC16CY_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354762.1"
                     /translation="AEMLKIIVENPQFTWLRPLSELIARIDALLDNVEPDVVVDFSAI
                     VGQARLLTSPDEEGTPYARSYHAAMQQVPDVVFAHRRVTSLLRDVPTRETRH"
BASE COUNT          422 a          879 c          856 g          432 t
ORIGIN      
        1 ttgccgcgct gtcgctcgcc tcgatggccc ttctcgcgca ggcgcccatg acaaaccttg
       61 gctacaccga cacaccgatg ctgcccggtg ctccgtatcg cgtgcacgac cccgcgcggc
      121 cccaccctgt ggtcgtaacc cctggcgctc aggccggggc gcctccgtcc gacgcaatcg
      181 tgctcttcga cggccgcgac ctttcccggt ggaccgcggc gaagcaggca tggaaggtgg
      241 agaacggcta cgtcgaggtc gtcccggact caggcgacat gcggacgaag gacacgttcg
      301 gcgactcgca gctgcacgtg gagtgggcgg ccccggctga ggtacgcggg tcgagccaga
      361 atcgcggcaa cagcggaatc ttcctgcagg ggcgctacga agtacaggtc ctcgactcct
      421 tcgacaaccc gacctacgcc gacgggcagg cgggcgccat ctacggccag tggccgccgc
      481 tcgtcaatcc cgtccggaag ccgggcgagt ggcagtcctt cgatatcgtg ttcgaggcgc
      541 cacggctgga cggcgcgcgt ctcgtgacgc ccgcctatct cactgtgttc ctcaacggcg
      601 tgctgcttca tcaccgccgg gaagtcatgg gaccgacggt ccacagagcg ctggcgaagt
      661 acgcagcgca gccctccgag gatgcgctgg tccttcagga tcaccagcag ccggtccggt
      721 atcgcaacat ctggattcgg cgcctgcgcg ggtacgacag cgccgcgcca cgttagtcct
      781 tggtgcttgg tgcctggtcc ttggtcccca tgcggccgag aagcgcctgt gcttcggcac
      841 gaatggcctc gtcgtccccc cgggcaagca gcgccctgag caccgagacg gcgcggtcgt
      901 accgcttctg ctggacgagg acctcggcca gcatcaggcg gtacggatcg cgtgcaggcg
      961 ccagtcgaat ggcatgctcg agcagcgccg ctgcgcgtac gagatcggcg ccgtcaacgt
     1021 actcgatgta tcccagggtg gccaggacgt gtgccgagga tggctcgagc tccgccgccc
     1081 tggtcaacac cgctcgcgcg cgggcgagaa tcgcgacgac catgtccagg tccttctgtt
     1141 tcccctggag ttgctcggca agcgcgcgtc cgagcgcgcc ttgaaatgcg ccgttctgcg
     1201 gctcgatcgc gaccgctcgc tccagtctcg cgagtgcatc gtcgatccgc ccctccctca
     1261 gatcgaggag ccccagactc gctgacgctc tggcgttctg cccgtttgcc gacacgagcg
     1321 tctgcaggta ggcacggccc gcgtccggtg tcactcgtgc cagcaggtcg ccgagatatc
     1381 cttgcgcttc cggctccgcg atcgcatcgc cgcgcgcaac cggcgccgcc tccagcttct
     1441 cgggaaagtt caaccgcaag gtcctcagcg ggagattgcg aagatagttt cgaagctcct
     1501 gctcgagcac gcccggttcc gtcttgaaag cgcctcgaaa ggcctcgtcc agcggagtgc
     1561 cgcgttcgac gcgatcgagg tagtcggaca gctgccccga gcgcgacgcg ctgcccaccg
     1621 caaggtagtg aacgagcgcc caggattgcg cgtagaagat cccgcgccga tcgccttcgt
     1681 tgtagtgggg cgagtcctgc tcaaccgcga ggagctcgct cagcggcatg agcggacctc
     1741 gctggagcag gcggaggtgg ctgaacgacg gcctgatgat cgccgccctg ccgccgtcgt
     1801 cctcgtacat ctggtagaac tcggccagcc cttcgttgag ccacggcggg acgacgccga
     1861 tcgtgttggc gatcaggaag tgcgtgtatt cgtggaagac cgcacggcgt gaccgttcga
     1921 cgttcatcgc aatgtaggtc acgtcgtcgc tgaggtgcat gtagccgccc acgttctcga
     1981 tgggacggcc cttgtaccgc ggcttgaacg gcgtgaacgc gcggtccgac gaaaacacca
     2041 tcacgacggg cggcaccgcg gagctgtccg acgctccggg aagcacgagc gccatcacgt
     2101 tcctgaactg ctcgaggctg cgcgcgacgc ggtggatcgc gccttccggc gcgtcgccga
     2161 cgaagaggaa gttctgagat cgaagccgtg tccagctgac ggccgaggcc ggctgcgccg
     2221 cgacgctcgc gagcaccagc gcggccaggc ccaggcgcgc acgccgcacg gacgagcatc
     2281 gcattggggc gtccgtgtca gtggcgggtt tcgcgcgtcg ggacgtcccg gagcaggctg
     2341 gtcacgcgac ggtgagcaaa aaccacgtca gggacctgct gcatcgccgc gtgatagctc
     2401 cgggcatagg gcgtgccttc ctcgtccgga gaagtgagca gccgcgcctg cccgacgatc
     2461 gcactgaagt caacaacgac gtcgggctct acgttgtcca gcagggcgtc aattcgtgcg
     2521 atgagctccg acagcggccg cagccacgtg aattgcgggt tctcgacgat gatcttgagc
     2581 atctccgcc
//
LOCUS       DBNGSH010000567         8269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2942533, whole genome shotgun sequence.
ACCESSION   DBNGSH010000567 DBNGSH010000000
VERSION     DBNGSH010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2942533"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC16CY_13030"
     CDS             <1..190
                     /locus_tag="AC16CY_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354763.1"
                     /translation="SCRTSTACGSGEAGAGVERLAGMPRLRAAPLAPHRGETPVAAVL
                     PEPVEDNAEAEAVAEACS"
     gene            181..744
                     /locus_tag="AC16CY_13035"
     CDS             181..744
                     /locus_tag="AC16CY_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354764.1"
                     /translation="MQLTPKAEALRAAYRAPEDSETANCLPPGMPGIMNQPYPMEFLL
                     TPGKVTIVIEAYTQVRHIYTDGRPLPADPNPEFHGTSIGRWEGDTLVVETIGFSPLTR
                     ITGGVPPSDKMRIVERFSLTGPDTMSIETTITDPENLLAPYVTTGTLRRHREWTITEY
                     ICEENNRNSVDAKGKAGIDLTVPASPR"
     gene            756..1145
                     /locus_tag="AC16CY_13040"
     CDS             756..1145
                     /locus_tag="AC16CY_13040"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354765.1"
                     /translation="MNIRTTLLAGLVLAGFSATVLPVAAHHSFSVFNTSETKTVTGTV
                     KEIEWTNPHIWLWVNVTNDKGGVDVYAFEGMSPNFLERRGWTRTTLKAGDKITVDYNP
                     LRDGKNGGMFRTGRMENGKTLTMGGSQ"
     gene            complement(1182..2975)
                     /locus_tag="AC16CY_13045"
     CDS             complement(1182..2975)
                     /locus_tag="AC16CY_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3354766.1"
                     /translation="MHTNPTKLRLWPGVAIVILQWVARFVIPLIVPDFTQYGVIAGLA
                     GGLALIVWWVFFSRARWADRLGALVVMVVAMAATWPFLDASIATGAMGFIFPILAVPG
                     LCLAFVLWAVTCGHLADGPRRLTMAVAVLLACGVWTLVRTGGFTGSFDNDLAWRWTAT
                     PEQRLLAQSEETVPAPQQSVADARQAPQASSTATPAESAAVEASARPAPSRAESRDWP
                     GFRGRNRDGVVHAVRINTNWSASPPVELWRRPIGPGWSSFAVAGDLLYTQEQRGDEEI
                     VSAYRVSTGAPVWKHRDAARFWESNGGAGPRGTPTVSNGRVYAFGATGILNALDAADG
                     RVIWSRNVAADCQRTVPEWGFSSSPLIVGDSVVIAAEGKLVAYDVATGVPRWFGPEGR
                     LGYSSPHLLTIDGVAQILLLNGGGASSVTPDGRTLWEHHWTSASLSAPIIQPASTEDG
                     DVLIGDGQATGLRRVAVRPGEGEWTVAERWMSSGLKPVFNDFVVHKGRAFGFDGSILA
                     CIDLSNGTRCWKGGRYGNGQLVLLPDQDLLLVLSEEGDVALVAAAIDRFTEIARFKAI
                     DGKTWNHPVLAGDVLLVRNGEEMAAFRLSLE"
     gene            3111..3899
                     /locus_tag="AC16CY_13050"
     CDS             3111..3899
                     /locus_tag="AC16CY_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354767.1"
                     /translation="MICRLRIRRLGLAAAAVVTFAFAGTAAAQWVYRNPGVPRTKDGK
                     PDLNTPVPRTAWGTVDLSGVWQTDIKYNANLAVDLKPGDVAMLPAGRVLFDERQGNLG
                     KDDPEGYCLAPGVPRVNGVPFPQKIVQTPALIVILYESRTMFRQIFLDAQHTLATDPQ
                     PAWMGYSKGRWDGDTLVVETTGFNGKTWLDDAGHPQSERARVSERIRRPDFSRLLVEI
                     TIDDPVMYAKPWTVTQEFQLEPDGELIEYICGENNRDIPHLVGK"
     gene            4020..4988
                     /locus_tag="AC16CY_13055"
     CDS             4020..4988
                     /locus_tag="AC16CY_13055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3354768.1"
                     /translation="MLLTTMVSVAGCGGNPVAPSAPTALPVNVPAAAPGKVAIISIDG
                     LRPDALLTAGAPNILALANRGAYSWQAQTVMPSRTLPSHVSMLSGFTPDAHGIVWDDY
                     IQERGPIPVPTLFSVARSLRRRTAVMAGKQKFRYFCEGAGSDVCAITANGDDEVTSGA
                     VSNRAADLLFVHLPDVDVNGHNTGWMSDAYLTAVRRADLAVGRIVSSLPADTTVILSA
                     DHGGHLSEHGSSQASDMTIPWIIAGPGIAQGRRLSSAVRTVDTAATAAYVLGFPLQPG
                     AAGRPVLEAFATPNQPVRAQGEAAVAQKTAAGLQTGQRRAAFLPDR"
     gene            complement(4897..6138)
                     /locus_tag="AC16CY_13060"
     CDS             complement(4897..6138)
                     /locus_tag="AC16CY_13060"
                     /inference="COORDINATES: protein motif:HMM:NF020337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HIR3354769.1"
                     /translation="MSDHHATSRVIRAACLLALLLLLRTGSAGAQTAATLFDDGVLHT
                     LKIAIHSRDWARLRSAYLENTHYPADLTWNGLRVTNVGVRSRGRFSRNGVKPGLTIDF
                     AHYSTRGQFLGLRALVLDNLVEDKSMIRERVAMAFLRRFGVPAPRAAFARVFVNGQYA
                     GLYAMVEEVDRVFAQHALGDANGALFEYRWLRPYFGEYLGDDLDLYRPLFEPRTREYD
                     SVFSLYDPIRELIRAINYAPAESFREAVGMRLDLAAMLRLLAAEVLLGEADGIASGTG
                     MNNFYLHRHSTNQRHQLLPWDRDLAFFQWNYPVTDGTSAHTLIRRALEDPELRSLYAG
                     FLVEAVQSATDADWLGQEVQRAYGQIREAVLADPVKPYTNEEFEAEAAVVIDLARRRP
                     AFVLSEVQRLFFGRQPPLPEP"
     gene            complement(6144..7370)
                     /locus_tag="AC16CY_13065"
     CDS             complement(6144..7370)
                     /locus_tag="AC16CY_13065"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR3354770.1"
                     /translation="MRNLACAGAFIVALALPAGVRAQSTSDDQATSKGASTQDGEAGV
                     FRMGDSPSLRLGDQARIDLHARVQADVGLQEEPGVAGDRFAWGNRRIGVDGVLFKRVE
                     FQIEREFSGVEPWRDVFADVRAHPALRIRAGHFKVPFSMERTTSVFDLDFIARAASVT
                     DISPGRETGIMLHGRDPRRLFEYEFGVFRLAEPPLQPLVESGTDGAMLTAARVTFTPG
                     TASIGIAFTGSRLPESLHAVSGHFDDDGLLSSEDFYVSGRRRRLAVEGRWTAGRLSIK
                     GELLRQADSREGESVSNSDLSDLIVGGGYVSGVWRLVGSPRRSRRAVDVAVRFERLGF
                     RSADQSDAPSLGPRADHIAPLRQDGWTFGGTWLVHRWVKVQVNAIRESFVDETGIRPE
                     IPQARWRSLMRLQFAM"
     gene            complement(7373..8083)
                     /locus_tag="AC16CY_13070"
     CDS             complement(7373..8083)
                     /locus_tag="AC16CY_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354771.1"
                     /translation="MRHFGSNAPTVGMKLVTPVADPPVAGQPPDPLSADEIKRRKKEW
                     KRHQDAARTLDAWERYRALVDASEEAFEVIDIANREARFALIVMGALNAVPLVVVSRA
                     DAFSVLSLGEQYVIGGLFLGYVVLVLTFVLGAIEALRPGRPHPALSDWPADTTDRPVG
                     IRYYEDIIRRSARENWAAWQTVRVSQLNAELAVQVHSLALKNHAKHVALRQLYAGLRV
                     MALALAGLSLLFVFLAWT"
BASE COUNT         1569 a         2728 c         2646 g         1326 t
ORIGIN      
        1 aagctgccgg acttcaacgg cgtgtgggag cggggaggcg ggggcggggg tggagcggct
       61 cgcggggatg ccgcgcctgc gcgcggcgcc gctggcgccg caccgcgggg aaacgccggt
      121 ggccgcggtg ctgccggagc cggtggagga caacgcggag gcggaggccg tggcggaggc
      181 atgcagctga cgccgaaggc cgaagcactg agagcggcgt accgcgcacc ggaggacagc
      241 gagactgcga actgtcttcc gccagggatg ccgggcatca tgaaccagcc ctatcccatg
      301 gagttccttc tgacgcccgg aaaagtcacg atcgtcatcg aggcctatac gcaggtccgg
      361 cacatctata cagacggcag accgctgccc gccgatccca accccgagtt ccacggcacg
      421 tcgatcgggc gttgggaggg tgacacgctg gtcgtcgaaa caatcggttt ctctccactg
      481 acacggatca ccgggggcgt gccgcccagc gacaagatga ggattgtcga acgtttcagc
      541 ctgacgggtc cagataccat gtcgatcgaa acgacgatca ccgatccgga gaatcttctc
      601 gctccctacg tcacgacggg cacgctccga aggcaccgcg agtggaccat caccgaatac
      661 atctgcgagg agaacaatcg gaactccgtg gacgccaagg ggaaagccgg aatcgatctg
      721 actgttcccg cgtctccccg atagcaggct caatcatgaa cattcgaacg acgctgctcg
      781 ccggactcgt cctagccggt ttctcagcca cagttttacc tgtcgccgcg catcactcgt
      841 tttcggtctt caacaccagc gaaacgaaaa cggtcacggg tacggtcaaa gagatcgaat
      901 ggacgaatcc ccatatctgg ctctgggtca acgtcaccaa cgacaagggt ggagtggacg
      961 tgtacgcctt cgagggcatg agtcccaact tcctcgagcg ccgcggctgg acgcggacca
     1021 ccttgaaagc gggcgacaag atcacggttg actacaaccc gctccgcgac ggaaagaacg
     1081 gcggtatgtt ccggaccgga cgcatggaga acggcaagac gttgacgatg ggcggcagcc
     1141 agtaggccga ttacctgtga gaccagaggc gcccggacac ctcattcgag cgacagcctg
     1201 aacgcggcca tctcttcgcc gttgcgaaca agcagcacgt cgccggcgag cacgggatgg
     1261 ttccacgtct tgccgtcgat cgccttgaac cgggcgatct cggtgaaccg atcgatggct
     1321 gccgcgacga gcgcgacgtc cccttcctcg gatagcacca gcagcaggtc ctgatcgggc
     1381 aggaggacga gctgaccgtt gccatagcgc ccgcccttcc agcaacgcgt gccgttcgag
     1441 aggtcgatgc acgccaggat gctgccgtcg aagccgaacg cgcggccctt gtggaccacg
     1501 aagtcgttga agaccggctt cagcccgctc gacatccagc gttccgcaac cgtccactca
     1561 ccctctccgg gccgaacggc aacgcggcgc agcccggtgg cctgaccatc gccgatcagg
     1621 acatcgccgt cctccgtcga agcgggctgg atgatgggag ccgacagact ggctgaggtc
     1681 cagtggtgct cccagagcgt acggccgtcc ggcgtcacgc tgctggcgcc gccgccgttc
     1741 aacagcagga tctgcgcgac gccgtcgatc gtcagcagat gcggcgagct gtaacccagc
     1801 cggccttccg gcccgaacca acggggcacg cccgtcgcga catcgtaggc aaccagcttg
     1861 ccctccgcgg cgatgacgac cgaatcgccg acgatgaggg gcgacgatga gaacccccac
     1921 tcggggaccg ttcgttggca gtcggccgcg acgttgcgcg accagatgac gcggccgtca
     1981 gcggcgtcga gcgcgttcag gattcctgtc gcgccaaacg cgtagacgcg gccgttgctg
     2041 accgtcggcg tcccgcgagg acccgccccg ccgttcgact cccagaaccg cgccgcatcg
     2101 cggtgcttcc acaccggggc gcccgtgctg acccggtatg ccgagacgat ctcctcgtct
     2161 cctcgctgct cctgtgtgta gagaagatcg ccagcgaccg cgaaggacga ccagccgggg
     2221 ccgatcggcc ggcgccacag ttcgaccggt ggtgaggccg accagttcgt gttgatccgt
     2281 acggcatgaa cgaccccgtc gcgattgcgt ccccgaaagc ccggccagtc ccgcgactct
     2341 gcccgtgatg gagcggggcg tgccgaagcc tcgaccgcag cgctttcggc aggagtcgca
     2401 gttgatgacg cctgaggcgc ctggcgcgcg tcagccactg actgctgagg agcgggcacc
     2461 gtctcctctg actgagccag cagccgctgt tccggcgtcg ccgtccaccg ccatgcgaga
     2521 tcgttgtcga aactgccggt gaaaccaccg gttcgaacga gcgtccacac gccgcaggcc
     2581 agcaggacgg ccacggccat tgtcagtcgt cgaggcccat ccgcaaggtg accgcaggtc
     2641 accgcccaga ggacgaaggc gagacagagg cccggaacgg cgagaatcgg gaagatgaag
     2701 cccatggccc cggtggcgat cgacgcatcg aggaagggcc acgtcgcggc catggcgacg
     2761 accatcacga cgagcgcgcc aagacgatcg gcccatcgcg cccggctgaa gaacacccac
     2821 cagacgatga gcgccagccc gccggcaagc ccggctatca cgccgtactg cgtaaagtcg
     2881 ggaacgatga gggggatgac aaaccgggcc acccactgca ggatcacgat ggccacgcca
     2941 ggccagagcc tgagcttcgt cgggttcgtg tgcacgccat catggacgca tctcaggagc
     3001 ggattgttcg ttacctccgc ctctgatctg caccagggct gcgtggctgt ggatcaactc
     3061 cccgtcgccc agtggcgtat tcttcgagaa atggtcgaag gtgcccgtct atgatttgcc
     3121 gacttcgcat ccgccggctg ggtcttgctg ccgccgccgt cgtgacgttc gcgttcgctg
     3181 ggaccgcggc ggcccagtgg gtttatcgca acccaggcgt tccacgaacg aaggatggca
     3241 agccggacct gaacacgccg gtaccgcgca ccgcctgggg caccgtggat ctctccgggg
     3301 tctggcagac tgatatcaaa tacaacgcga acctcgccgt cgatctgaag ccgggcgatg
     3361 tcgccatgtt gccggcagga cgcgtgttat tcgacgagcg ccaggggaac ctgggcaaag
     3421 acgaccccga gggctattgt ctggcgcctg gcgtgccgcg agtcaacggc gtgccatttc
     3481 cgcagaagat cgttcagacg cccgcgctca tcgtgatcct gtacgaatca cgaacgatgt
     3541 ttcgacagat ctttctcgat gcgcaacaca cgctggcgac cgacccgcaa cctgcctgga
     3601 tgggctactc gaaggggcgt tgggacggcg acacccttgt cgtcgagacg accggcttca
     3661 acggaaagac gtggctggac gatgccgggc atccacaatc ggagcgcgcc agggtgagcg
     3721 agcggatccg gcgtccggat ttctcgcgtc tcctcgtgga gatcaccatc gacgatcccg
     3781 tgatgtacgc gaagccgtgg accgtgacgc aggagtttca gctcgagccc gacggcgagc
     3841 tgatcgagta catctgcggc gagaacaacc gcgacattcc acacctggtc ggcaagtagg
     3901 cagcagcggc tggacaaccg ttcctttacg gcattcgacg attctccgta taatcgcggc
     3961 gggtcttcgt gggtctgaca cctggcactc gccgcggccc ttatgaagtc agcgccgcga
     4021 tgctgctcac gaccatggtg tcggtcgcgg gatgcggcgg gaatccggtt gcgccctccg
     4081 cgcccactgc actcccggtg aacgttcctg ctgcggctcc cggcaaagtc gccatcatct
     4141 cgatcgatgg actccgcccc gacgcgctgc tgaccgctgg cgcaccgaac attctggccc
     4201 tggccaatcg aggcgcgtac tcgtggcagg ctcagacagt catgccctcc aggacgttac
     4261 cgtcccacgt gtcgatgctc tcgggcttca cccccgatgc gcacggcatc gtgtgggacg
     4321 actacataca agagcgcgga ccgatcccgg tgccaacgct gttcagcgtc gcccgaagtt
     4381 tgcgacgccg cacggccgtg atggccggca aacagaagtt caggtacttc tgcgaagggg
     4441 ccggaagcga cgtctgcgcg atcacagcca acggagacga cgaggtgacg tcgggcgcgg
     4501 tgagcaatcg cgcagccgac ctgctcttcg tccatctccc cgacgtggac gtgaacgggc
     4561 acaacactgg atggatgtcg gatgcgtatc tgacggccgt tcgtcgggca gatctcgcgg
     4621 tgggccggat tgtgtcgtcg ctgcccgccg acaccacggt gattctctcg gccgaccacg
     4681 gcggacacct cagcgaacac ggatcgagcc aggcgtcgga catgacgatt ccgtggatca
     4741 tcgccgggcc tggaatcgcg caggggcgcc ggttgtcctc cgcggttcgc accgtcgaca
     4801 ctgcggcaac ggcggcctac gtcctcggct ttccgcttca accgggcgcg gcgggtcggc
     4861 ccgtgctcga agcgttcgcg acgcccaatc agcccgtcag ggctcaggga gaggcggctg
     4921 tcgcccaaaa aacagccgct ggacttcaga caggacaaag gcgggccgcc ttcttgccag
     4981 atcgatgacc acggccgcct ccgcctcgaa ctcctcgttc gtatagggct tgaccggatc
     5041 ggcgagcacc gcctcgcgaa tctgcccgta cgcgcgctgg acctcctgcc cgagccagtc
     5101 ggcatcggtc gccgactgta ccgcctcgac gagaaagccg gcgtagagac tgcgcagttc
     5161 cggatcttcg agcgcccgcc gtatcaatgt gtgagcagac gtaccgtccg tgaccgggta
     5221 gttccattga aagaaagcaa ggtccctgtc ccacggcagc aactgatgcc gctggttcgt
     5281 gctgtgacgg tgcaggtaga agttgttcat gcccgtacca ctggcgatgc cgtcggcctc
     5341 gcccaggagc acctcggcag caagaaggcg cagcatcgcg gcgagatcga ggcgcatccc
     5401 gacggcctcg cgaaagctct ccgctggcgc atagttgatc gcgcgaatca actcccggat
     5461 cggatcatag aggctgaaaa ccgagtcgta ttcccgggtt cggggttcga agagcggccg
     5521 gtacagatcc aggtcgtccc cgaggtactc gccgaagtac ggccgcagcc accgatattc
     5581 gaagagggct ccgtttgcgt cgccgagcgc gtgctgggcg aacacacggt ccacctcttc
     5641 gaccatcgcg taaagtccgg catactggcc gttgacaaat accctggcga acgccgcacg
     5701 aggagccgga acgccaaagc gccggaggaa cgccatggcc acgcgttccc gaatcatcga
     5761 cttgtcctca accagattgt cgagaacgag cgcccgcagt cccaggaact gacctcgcgt
     5821 gctgtagtga gcgaagtcga ttgtcagacc aggcttgacg ccgttgcgtg aaaagcggcc
     5881 gcgcgaccgc acgcccacgt tggtcacacg gaggccattc cacgtcagat cggccggata
     5941 gtgggtgttc tcgagatacg cgctgcgcag ccgagcccag tcgcgggagt gaatggcgat
     6001 cttcagcgta tgcagaaccc cgtcgtcgaa cagggtggcc gcagtctggg cgcccgctga
     6061 ccccgtgcga aggagcagga ggagcgcgag cagacaggca gcgcggatga cgcgagacgt
     6121 tgcgtggtgg tctgacatag gagtcacatc gcgaactgaa gtcgcatcag gctcctccac
     6181 cgagcctgcg ggatctcggg tcgtatccct gtttcgtcga cgaatgactc gcggatcgcg
     6241 ttgacttgca ccttgaccca gcggtgcacc agccaggtgc cgccgaacgt ccacccgtcc
     6301 tgacgcagcg gtgcgatgtg atcggctcga ggaccgaggg atggtgcgtc ggattgatcg
     6361 gcgctgcgga agccgagacg ctcgaatcgg acggccacgt ccaccgctcg tctcgaccgt
     6421 cgcggggacc cgacaaggcg ccacacaccg ctgacgtatc cgccgccgac gatgagatcc
     6481 gacaagtcgc tgttcgagac agattcaccc tctcgtgaat cagcctggcg gagaagttcg
     6541 cctttgatcg aaagcctccc ggccgtccat cggccctcga ccgccagccg ccgccggcgg
     6601 ccgctgacgt agaagtcctc ggaggacagc aggccatcgt cgtcgaagtg gcccgatacg
     6661 gcatgaaggc tctcgggaag acggctcccg gtgaacgcga ttccgatgct cgcggtgccg
     6721 ggcgtaaaag tgacgcgcgc ggccgtgagc atggcgccgt ccgtcccgct ctcgacgagt
     6781 ggctggagtg gaggctcggc gagcctgaac acgccgaact cgtactcgaa gagccggcgc
     6841 ggatctcgcc cgtgcagcat gatgcccgtc tcacgccccg gcgagatgtc agtgaccgag
     6901 gcggcccgag cgatgaaatc caggtcgaat acactcgttg tccgttccat gctgaacggc
     6961 accttgaaat gtccggcccg gatgcgcaat gcggggtgcg cacgcacgtc ggcaaacaca
     7021 tcgcgccatg gctcgacgcc cgagaactcc cgctcgattt gaaactcgac ccgcttgaac
     7081 aggacgccat caacgccgat gcgacggttc ccccacgcga agcggtcgcc tgcaaccccg
     7141 ggctcttcct gcaggcccac gtcggcctga acgcgagcgt gcaggtctat tcgcgcctga
     7201 tcgcccagac gcaatgaggg gctgtcgccc atcctgaaca cgccggcctc gccgtcttgt
     7261 gtcgacgctc ccttggacgt cgcctggtcg tcggacgtgg actgcgcacg caccccggca
     7321 ggtagggcaa gcgctacgat gaatgccccg gcgcaagcga ggttccgcat ggctacgtcc
     7381 acgccaggaa cacgaagagc aggctcagcc cggcaagtgc cagagccatg acgcgaagcc
     7441 cggcgtagag ctggcgcagt gcgacgtgct tggcgtggtt cttcaatgcg aggctgtgca
     7501 cctgtaccgc cagttcagcg ttgagctgcg acacgcggac cgtctgccac gccgcccagt
     7561 tctcgcgagc cgaccggcgg atgatgtcct cgtagtacct gatcccgact gggcggtccg
     7621 tggtgtcggc gggccagtcc gagagcgctg gatgaggacg cccgggcctg agcgcttcga
     7681 tcgcgcccag cacgaacgtc agcacgagca cgacataacc caggaagagc cccccgatca
     7741 cgtactgctc gcccagagac agcacggaaa acgcgtctgc ccgtgagacc acgacaagcg
     7801 gtaccgcgtt gagggcaccc atcacaatca aggcgaagcg tgcctcgcga ttcgcgatat
     7861 caatgacctc gaaggcctcc tcgctcgcgt cgaccaaggc acgatagcgc tcccaggcat
     7921 cgagcgttcg ggcagcatcc tgatggcgtt tccactcctt cttccggcgc ttgatttcat
     7981 cggccgacag cggatcggga ggctggcccg ccaccggcgg gtcggcgacc ggcgtcacga
     8041 gcttcatccc cacagtgggg gcattcgaac caaagtgcct catctttaat cccctgccgg
     8101 gctagcggct ccccgagggc cgttcgccgt tggagccctg tacagaggac taacgttgcg
     8161 cgtgggactt gcgaacggag gagccattgc cgtgcggacg ggaggaccta atcacataga
     8221 atctccccgg ccacgtgccc gacaggagat aacaacatgc gacggttct
//
LOCUS       DBNGSH010000568         2887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_56525, whole genome shotgun sequence.
ACCESSION   DBNGSH010000568 DBNGSH010000000
VERSION     DBNGSH010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_56525"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="AC16CY_13075"
     CDS             complement(<1..569)
                     /locus_tag="AC16CY_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate family dehydrogenase"
                     /protein_id="HIR3354772.1"
                     /translation="MSTVCIIGAGDLGGAVAHALASGERVARVVLIDDAAGVASGKAL
                     DIQQAGAIEGFHTRLEGTSDRARVAGCSVCVIADRSGRPSSEWQGEEGLAMIRRLAAH
                     LGDSPLVFAGVSQADLLLPSWWEAGLQRERLIGSAAEALASAVRSMVALEARCSPREI
                     GLTVLGAPPRGFVVPWSEASIGGYALERVL"
     gene            622..1326
                     /locus_tag="AC16CY_13080"
     CDS             622..1326
                     /locus_tag="AC16CY_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3354773.1"
                     /translation="MQLSGKVAFVTGGSRGIGLATAKALIGQGASVAITGTDQARLNG
                     AASELGANALGIRADVRDYPQVERAISTAVSRFGGLDILVNNAGVGVFRPVAETTVDE
                     WRNVIDTNLSGVFYCCHAALPHLKARGGGWIINISSLASKNAFVNGAAYCASKSALNS
                     FSEALMQEVRYDGVRVAYVLPGSVNTGFGGLSNTKSQWALMPDDVAEVIVDLIAHPSR
                     SLPSRVEIRPAQPPRK"
     gene            1355..1609
                     /locus_tag="AC16CY_13085"
     CDS             1355..1609
                     /locus_tag="AC16CY_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354774.1"
                     /translation="MPRDAFDKHHDELEKHETMMGQARGRLAVALDLLTDALAMVGQH
                     GVYCQSARTPGRPALDIAMVIEQIGDAKELVQSVIDAERA"
     gene            1606..2517
                     /locus_tag="AC16CY_13090"
     CDS             1606..2517
                     /locus_tag="AC16CY_13090"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3354775.1"
                     /translation="MNSPAPAPIGVVAHYNLLERLEPAGPGDLYRARDTRLGRTVAVR
                     VLPADFASDRRSLIEGARGLSALSHPNTTALFDVGEHEGRVYLVFEFQKGQSLRAESA
                     GRAMNVRRAVEVAIQIADAVADAHAHGFGHGGLSPDSVVISAKGRAKIPAFELASQSG
                     FNRNGNDGRLIDYDAPEEARGERPDDRSDVYSIGAILYETLTTRRPLHRGASAPSASN
                     PKVPTEIDDIVLKALAPNPDARYQSAVAFAGALRGSIAVLDALGVAGEEEELVQAQST
                     SVGRVAAMAGVMLLVAAAFVWWFFMRS"
     gene            complement(2504..>2887)
                     /locus_tag="AC16CY_13095"
     CDS             complement(2504..>2887)
                     /locus_tag="AC16CY_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354776.1"
                     /translation="GLRASCDYPINMIVCVVDDLIFSIKISTAAKRLGVEMYFERSKD
                     NVLPTVREKQPSLVIFDLNSGRLAPMDAIAAMKADPALKTIRTLGYVSHVDSDAIAAA
                     RRAGIDQVLARSAFSDGLGEILTSA"
BASE COUNT          486 a          978 c          979 g          444 t
ORIGIN      
        1 agaacgcgct ccagagcgta gcccccgatc gaagcctcgc tccacgggac gacgaagcct
       61 cttggcggag caccgagaac cgtcagaccg atttcgcgtg gcgagcagcg cgcctcgagc
      121 gcaaccatcg acctgaccgc agatgcgagc gcttcagcgg cagaaccaat cagccgctcc
      181 cgctgaagac cagcctccca ccacgatggc aacaggagat cggcctggga tacaccggcg
      241 aacacgagcg gactgtcgcc gaggtgcgcc gccagtctgc ggatcatcgc caggccctct
      301 tcgccctgcc attcagacga gggcctgccg ctgcgatcgg cgatgacgca gaccgagcag
      361 ccggcgaccc tggcgcgatc gctcgttcct tcgaggcgtg tgtgaaaccc ttcgatcgct
      421 cccgcctgct ggatgtcgag cgcctttccc gatgccacac ctgcggcgtc gtcgatgaga
      481 acgacccgtg ccacacgctc gccgctcgcg agcgcgtgcg cgacagcccc gccgagatcc
      541 ccggccccga tgatgcagac ggtcgacatg agttgctgga gtattctagc tagctggaag
      601 gtcgtggctg gtagccggta gatgcaactc tcaggaaagg tcgcgttcgt cacgggtggc
      661 tcgcggggta ttggtctggc cacggccaag gcgctgatcg ggcagggagc ttcagtggcg
      721 atcacgggaa ccgatcaggc gcggctgaac ggggcggcgt cggagcttgg cgccaacgcg
      781 ctgggcatcc gcgccgatgt acgggattac ccgcaggtcg agcgtgcgat ctcgacggcg
      841 gtgtcccggt tcggcggcct cgacatcctc gtcaacaacg ccggcgtcgg cgtgtttcgg
      901 ccggtggccg agacgaccgt cgatgaatgg cgcaacgtca tcgacaccaa cctgagcggc
      961 gtcttctact gctgtcacgc cgccctgccg cacctgaagg cccgcggggg agggtggatc
     1021 atcaacatca gcagcctggc cagcaagaac gcgttcgtga acggcgcggc gtactgtgcg
     1081 tcgaagtccg cgctgaactc gttctccgag gcgctcatgc aggaagtgcg ctacgacggc
     1141 gtgcgcgtcg cctacgtgct gccgggctcg gtaaacaccg gcttcggggg gctttcgaac
     1201 acgaagtcgc agtgggccct gatgccggac gacgtcgcgg aggtcatcgt cgatctgatt
     1261 gcgcacccgt cgaggagcct tcccagcaga gtcgagatcc ggcccgcgca gccgccacgg
     1321 aagtaggccg ttcacagttc acagcaggca gcggatgccc agagacgcct tcgacaagca
     1381 ccacgacgaa ctcgagaagc acgaaacgat gatggggcag gcgcgcggcc ggctggccgt
     1441 ggcgctcgac ctgctcaccg acgcgctggc gatggtgggg cagcacgggg tgtattgcca
     1501 gagcgcgcgg acgccggggc ggccggcgct ggacatcgcg atggtgatcg agcagatcgg
     1561 cgacgcgaaa gagctcgtgc agagcgtgat cgacgccgaa cgcgcatgaa ctccccggcg
     1621 cccgctccga ttggcgtcgt cgcgcactac aacctcctcg agcggctcga accggcgggg
     1681 ccgggcgatc tctatcgcgc gcgcgacacc cgcctcgggc ggacggtcgc cgtccgggtg
     1741 ctgccggcgg acttcgcgtc cgaccggcgc tcgctgatcg agggcgcgcg gggcctgagc
     1801 gcgctgtcgc atccgaacac gaccgcgctg ttcgacgtcg gcgagcacga aggccgcgtg
     1861 tacctcgtct tcgagtttca gaaaggacag tcgctccgcg cggagtcggc gggccgcgcg
     1921 atgaacgtcc gccgcgcggt cgaagtggcg attcagatcg cggacgccgt cgccgacgcg
     1981 cacgcccacg gcttcgggca cggcgggctc agccccgact cggtcgtcat cagcgcgaag
     2041 ggacgcgcca agatccccgc cttcgagctc gcgtcgcaga gcgggttcaa tcgcaacggc
     2101 aacgacggcc gcctgatcga ctacgacgcc cctgaggagg ctcgcgggga acgacctgac
     2161 gatcgctccg acgtctactc gatcggcgcc atcctctacg agacgctcac cacccgtcgt
     2221 ccgctgcacc ggggcgcgtc ggccccgagc gcgtcgaacc cgaaggtgcc gacggagatc
     2281 gacgacatcg tgctgaaggc gctggccccc aaccccgacg cgcgatacca gagcgcggtc
     2341 gcgtttgccg gcgcgctgcg cggatcgatc gccgtgctcg acgcgctcgg cgtggccggc
     2401 gaggaagagg agctggtcca ggctcagtcc accagcgtcg ggcgcgttgc ggccatggct
     2461 ggcgtcatgc tgctcgtggc ggccgcgttc gtctggtggt ttttcatgcg ctcgtgagga
     2521 tctcgcccag accgtccgag aacgccgatc tcgccagcac ctgatcgatc cccgcccgcc
     2581 gcgccgccgc gatggcgtcg gagtccacgt gcgagacgta gccgagcgtc cgaatcgtct
     2641 tgagcgcggg gtcggccttc atggcggcga tcgcgtccat gggcgcgagc cgcccgctgt
     2701 tcagatcgaa tatcacgagc gacggctgct tctccctgac ggtcggcagc acgttgtcct
     2761 tcgagcgttc gaagtacatc tccacgccca ggcgcttggc cgccgtcgag atcttgatcg
     2821 agaagatcag gtcgtccacc acgcacacaa tcatgttgat aggatagtcg catgatgctc
     2881 gaagtcc
//
LOCUS       DBNGSH010000569         5036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2093919, whole genome shotgun sequence.
ACCESSION   DBNGSH010000569 DBNGSH010000000
VERSION     DBNGSH010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2093919"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="AC16CY_13100"
     CDS             complement(<1..872)
                     /locus_tag="AC16CY_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR3354777.1"
                     /translation="MSDDRSTNKRSLPGAGVTRRQVPLSAHDIVTEQPLASGRWFPRV
                     VRPALDGVDLVAWLGQNRQQIQEKLQRDGAILFRGFDVSSVDKFEQVITAACGQALRY
                     TERSSPRSEVKGNVYTSTDYPPAYRIFLHNENSYARTWPQKLLFCCIKPSTERGETPI
                     ADTRGVYNRIDPKIRDRFIEKKVMYVRNFSPGLGLAWSTVFGTTDRGEVEAYCRTAGY
                     ETEWLDSERLRTRRVGQAVARHPKTGDTTWFNHATFFHVSTLEPAVRDALLAQFAEDE
                     LPNNTYYGDGTPIEA"
     gene            complement(869..>5036)
                     /locus_tag="AC16CY_13105"
     CDS             complement(869..>5036)
                     /locus_tag="AC16CY_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIR3354778.1"
                     /translation="QYADFAAWQRNWLSGAVLADQIEYWKSQLSGAPPSLDLPTDRPR
                     PAVQTFRGARQRVRVSGPITRALKSLNRRQGTTLFMTLLTAFQALIHRWTDQTDILVG
                     VPIAGRQQAETQGLIGFFVNTLVMRGRVRPDAPFQELLAETRDVAMQAYAHQDLPFEK
                     LVEGLGGERDLSRTPLFQVVFALQNAPFKPLDLAELRLEPFEVEQVTAKFDLTLNLAE
                     SQDGIQGSLEYNTDLFDAATMERMAGHFETLLEGIVANPAQRVSELPLMSQPERQRLL
                     VEWNATEAALPEDGLIHELVEAQAEKRPDAVAVAYQGRLLGYRELNARANQLARHLRK
                     LGVGPEVPVGILMERSSAMVEGMLGVLKAGGTYLPLDPEAPSDRLQFMIGDAGIRVVL
                     TVESLESDLPAVAHVVCLDRDRRAISQQGKENLESRVDNGNLAYVIYTSGSTGRPKGV
                     GVPHGGLRNLVAWHQKVFGLTPSSRATQIAAPTFDASVWEIWPYLAAGSSVHIPDDVT
                     RNSPADLALWLTTENVTHTFMPTPLAEEVLQEPWPLQTSLEFFLTGGDELRRVPKQAL
                     PFRLTNQYGPTENTVVTTWTPVESTRTVEKPPPIGRPIDNVRVYLLDRRLNAVPVGVN
                     GELYIGGSSLSRGYASRADLTAERFVPDPFSAKPGARLYRSGDLCRFSPVGDLEFQGR
                     IDYQVKIRGFRIELGEIEAVLGQFPGVRQAVVLARPDTRGSARLVAYVVPHDGSPDVM
                     ALRSYLKASLPEYMVPTAFVTLDTLPLTSNGKVNRRALPAPQVSATEETYLAPRNHHE
                     KRLADMFAEVLGMDRVGVHDNFFEIGGHSLLATKLVSRIRAAFDTRFPLRRVFESPTV
                     FELAKSLTDEETPSRVTPGEELPLLERTRQEGPLPLSFAQQRLWFLDRMEPGNPYYNM
                     PTALRVSGDLRVSALEQAIDEIIRRHEVLQNCYVLQQGQPRQVRMPRRDLTLPIVDLS
                     SLQGASREEHERLLAAADARRPFDLATGPVVRAALLKKGEGSYTILFAMHHVVSDGWS
                     VAIVVRELSALYEALGAGRPSPLPELPIQYADFAVWQRRWLESDGLKSQLSYWKARLG
                     GHLPTLAIRTDRPRPEIESFRGADEILLIPQALAGALNRLSAREHVTLFMTLVAAYKT
                     LLYRNVGQDDIVVGTDLAGRNQAELEGLIGFFVNLLVLRTDLSGNPTFRALLARVRET
                     AMGAFAHQDVPFDRLVEELQPKRHRSRTPLFQMLFVMENIPMETLRLPGLTMTPMPNQ
                     ADTARFDLALFVREQDGGLVTKWTYKTDLFDRGTIQRMANQYVALLEHIAAAPDTRVN
                     ELALQTQAEIRDQALEEQRRREAGMSSLVPGRRRARKLPETPGGTETNRAGAEDDAAV
                     GDASKQVRTR"
BASE COUNT          909 a         1631 c         1580 g          916 t
ORIGIN      
        1 gcctcgatcg gggttccgtc gccgtagtag gtgttgttcg gcaactcgtc ttccgcgaac
       61 tgggccagca gcgcgtcgcg gactgccggc tcgagcgtgg acacatggaa gaacgtcgca
      121 tggttgaacc aggtcgtatc gcccgtcttc ggatggcgcg cgacggcctg gccgacgcgg
      181 cgggttctca ggcgctcgga gtccagccac tccgtctcat agcctgccgt cctgcagtac
      241 gcctccacct cgcctcgatc cgtcgtcccg aagaccgttg accaggccag ccccagcccc
      301 ggactgaagt tgcggacgta catcaccttc ttctcgatga aacgatcccg gatcttcggg
      361 tcgatccggt tgtagacacc gcgagtatcg gcaatgggtg tttcgcctct ctccgtagag
      421 ggcttgatgc agcagaacag cagcttctgg ggccaggtcc gcgcgtatga gttctcgttg
      481 tggagaaaga tccggtaggc agggggatag tcagtggacg tgtagacgtt gcccttgact
      541 tccgatcgcg gcgacgagcg ctccgtatat ctcagcgcct gaccgcaggc ggccgtgatg
      601 acctgctcga acttgtccac cgaggacacg tcgaacccac ggaacaggat cgcgccgtct
      661 cgctgcagct tctcctggat ctgttgccgg ttctgcccga gccaggcgac gagatcgacc
      721 ccgtcgagcg ccggtcgcac gacgcgggga aaccaccgac cggacgcgag gggctgctcg
      781 gtcacgatat cgtgggccga cagaggcacc tggcggcggg tgacgcccgc gcccggcagg
      841 ctccgtttgt tcgtggaccg atcgtcgctc atcgagtcct cacctgcttg cttgcatcgc
      901 cgacggcggc atcgtcctcc gccccggcgc ggttagtttc tgtaccgccc ggtgtctccg
      961 ggagctttct ggctcgacgg cggccgggca ccaacgaaga catcccggct tctcgacgac
     1021 gttgctcttc gagcgcctga tcgcggattt cggcctgcgt ctgcaacgcg agctcgttca
     1081 cccgcgtgtc cggagcagcg gcaatgtgtt cgagcaacgc gacatattgg tttgccatcc
     1141 gctggatcgt gccgcggtcg aacaggtctg tcttgtaggt ccacttcgtg acgaggccgc
     1201 catcctgttc ccgcacgaac agcgccaggt cgaacctggc cgtatcggcc tggttcggca
     1261 tgggcgtcat cgtcaggccc ggcagccgca acgtctccat cggaatgttc tccatcacga
     1321 aaagcatctg gaagagcggc gtccggctgc gatgccgctt gggctgcagt tcctcgacaa
     1381 gcctgtcgaa gggaacatcc tgatgagcga aggcccccat tgccgtctcg cggactcgcg
     1441 cgagcaatgc tcgaaaggtc ggattcccgc tcaagtcggt tcgcagcacc aataggttga
     1501 cgaagaagcc gatcaagcct tcgagttccg cctggtttct gccggcgagg tcggtgccaa
     1561 cgacgatgtc gtcctgaccg acgtttcgat acaggagagt cttgtaggcc gccaccagtg
     1621 tcatgaacag cgttacgtgc tcgcgagccg acagacgatt caacgctcca gccagcgcct
     1681 gtgggatgag cagtatctcg tcggctcctc ggaacgactc gatctccggc ctgggccgat
     1741 cggtgcgaat ggcgagcgtg ggaaggtgac cgccgagtcg ggccttccag taggacaact
     1801 gagacttcag cccgtcgctc tccagccacc tgcgttgcca gacggcaaaa tctgcgtact
     1861 gaatcgggag ctccggcaat ggcgacggac gtccagcacc aagggcctcg tacaacgcgg
     1921 acaattcgcg aacgacaatg gcaaccgacc agccatcaga cacgacatgg tgcatggcga
     1981 agagaatggt gtaggagccc tcgcccttct tgagcagcgc ggctctcacg actggcccgg
     2041 tggccaaatc gaagggccgt cgggcatcgg cggcggcgag aagccgctcg tgttcctcac
     2101 gcgaagcccc ttgcaacgag ctgagatcaa caatgggcag cgtgaggtcc cggcgcggca
     2161 tccggacttg cctgggctgt ccctgctgaa gcacgtaaca gttctgcagc acctcgtgcc
     2221 ggcggatgat ttcgtcgatc gcctgttcga gcgcggagac ccgcaagtcg ccgctcacac
     2281 gcaacgccgt cggcatgttg tagtacggat ttccaggttc catccggtcg aggaaccaca
     2341 gccgctgttg agcgaaggag agcgggagcg gtccttcctg gcgggtccgt tcgagcagcg
     2401 gcagctcctc cccaggcgtg acgcgcgacg gcgtctcttc atcggtgagg ctcttggcca
     2461 attcgaagac ggtgggactc tcgaagacgc gacgcaaagg aaaccgcgtg tcgaacgcgg
     2521 cacggatacg ggacacgagc ttcgtggcca gcagggaatg cccgccgatc tcgaagaagt
     2581 tgtcgtggac acccacgcga tccatgccga gcacttcggc aaacatgtcc gcgagccgct
     2641 tctcgtggtg attgcgcggg gcgaggtacg tctcctcggt ggcgctgact tgcggtgccg
     2701 ggagggcacg ccggttcacc tttccgttgg atgtcagcgg caacgtatcg agcgtcacga
     2761 acgccgtcgg taccatgtac tcgggcagcg acgccttcaa gtaactcctc agcgccatca
     2821 cgtccggtga cccatcgtgg ggcaccacgt aggcgacgag ccgcgccgac ccacgcgtgt
     2881 ccggtctggc caatacgacc gcctgccgaa caccggggaa ttgtcccagg accgcctcga
     2941 tctcgcccag ttcgatacgg aagccccgga tcttgacctg ataatcgata cggccctgaa
     3001 actcgagatc gcccacgggt gaaaagcggc agaggtcgcc acttcgatac aggcgagccc
     3061 ccggcttcgc gctgaacggg tcggggacga atcgttcggc ggtgagatcc gcacgggacg
     3121 cgtaccctcg gctgaggctg gacccgccaa tgtacagctc tccgttgacc ccgacgggga
     3181 cggcattcag gcgccgatcc agaaggtaaa cacgaacgtt gtcgatgggg cgcccgattg
     3241 gcggcggctt ctcgaccgtc ctggtgctct cgacgggcgt ccacgtggtc acgacagtgt
     3301 tttctgtcgg tccatactga ttcgtgagtc gaaacggcaa tgcctgcttc ggaacacgtc
     3361 ggagctcatc gccaccggtc aggaagaact cgagggatgt ctgcagcggc catggctcct
     3421 gcaggacttc ctcggccagc ggcgtcggca tgaacgtgtg ggtcacgttc tccgtggtga
     3481 gccacagcgc gagatcggcc ggtgaattgc gggtcacatc gtccggaata tggacgctcg
     3541 aaccggcggc cagatacggc cagatctccc agacggaggc atcgaatgtc ggcgcagcga
     3601 tctgcgtcgc acggctcgaa ggcgtgaggc cgaacacctt ctggtgccag gctacgaggt
     3661 tgcgcagccc gccgtgcggc acaccgacgc ccttgggtct tccagtggac cctgacgtgt
     3721 agatgacgta cgccagattg ccattgtcga cgcggctttc caggttctcc ttgccctgct
     3781 gcgagatggc tcgccggtct ctatccagac aaacgacgtg ggccacggcc ggcagatccg
     3841 actcgaggct ctcaacggtg agaacgactc gtatacccgc atcgccgatc atgaactgca
     3901 ggcggtctga cggggcctcc ggatccaaag gcaggtaggt tccgccagct ttcagcacgc
     3961 ccagcattcc ctccaccatc gccgacgacc gctccatcag aatcccgacg ggcacctccg
     4021 ggccaactcc cagctttctc aaatggcggg cgagttgatt ggcccgagcg ttcagctcgc
     4081 gatagccgag cagccgccct tggtacgcca cggccacagc atccggcctc ttctcggctt
     4141 gtgcctcgac caactcatgg atgagaccat cttcaggtag agccgcttcg gtcgcattcc
     4201 actccaccag caaccgctgc cgctcaggct gggacatcag cggcagttcc gagacccgct
     4261 gcgccggatt cgccacgatc ccctcgagca gggtctcaaa gtgtcccgcc atgcgctcca
     4321 tcgtcgccgc gtcgaacaga tcagtgttgt attccagcga tccctgaatg ccgtcctgtg
     4381 actcggcgag gttcagcgtc aagtcgaact tcgccgtcac ctgctcgact tcgaacggct
     4441 ccagccgcag ctcggcgaga tcaagcggct tgaacggagc gttctggagc gcgaacacca
     4501 cttggaagag cggtgtccgg ctcaagtccc gttctcctcc cagtccctcc accagcttct
     4561 cgaacggcag gtcctggtgc gcataggcct gcatcgcgac gtcgcgcgtc tcggcgagca
     4621 gttcctggaa gggcgcatcg gggcgcacgc gcccgcgcat gacgagcgtg ttgacgaaga
     4681 agccgatcag cccctgcgtt tcggcctgct gccgcccggc aatgggaacg ccaacgagga
     4741 tgtcggtctg gtccgtccat cgatgaatga gcgcctgaaa agccgtcagg agcgtcatga
     4801 acagcgtcgt gccctgccgc cggttcagtg acttcagggc tcgagtgatc ggaccggaga
     4861 cgcggacgcg ctgacgcgcg ccgcggaagg tctgaacggc cggccggggc cgatcggtcg
     4921 ggagatctag cgagggcggc gcaccgctca actgcgactt ccagtactcg atctggtctg
     4981 cgagcacggc tcccgacagc cagttccgct gccaggcggc aaaatcagca tactga
//
LOCUS       DBNGSH010000570         1838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1754543, whole genome shotgun sequence.
ACCESSION   DBNGSH010000570 DBNGSH010000000
VERSION     DBNGSH010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1754543"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC16CY_13110"
     CDS             complement(<1..307)
                     /locus_tag="AC16CY_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354779.1"
                     /translation="MSRAFVTCVCTVIALNLTILAQAPPARGTGDQRRQYVFAPTGQQ
                     MPYRVYVPKTWDGKASLPIILMLHGAGANEGTYLDQAEGLLMKLAEQHRYIVVSPLGF
                     "
     gene            complement(304..1509)
                     /locus_tag="AC16CY_13115"
     CDS             complement(304..1509)
                     /locus_tag="AC16CY_13115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3354780.1"
                     /translation="MTTTVLPAVSTNWLLPFGATLIAMFTLQLSNLGFSPLLPSIQQE
                     FGMSYTQLGFFTGLYGLLAMLLSVPAGVSARRFGEKRVLGFGLLGVAAGSVLLGDAWS
                     FGSATAFRGLTIFGYRFAFVSVLIAVALTAPPSLRGRTMGVLGATSALASVVGAPLGG
                     VLVGEFGWRLAILGYAAMAVLGATVFWLFYRPTSDDPTSAGHAGHGAGSGSAFLSPVV
                     WMLALVVGLGGFGQFTVTYFVPSVAAALYGLDATAAGLIISTGYLTAIAVNLGVGLLA
                     DRFNKLVVLGAVFILLAVASASMAVESLAIFRVATAAVIGFGFTAANQLYGLAGLVMP
                     RHEAGHAMGVVSLGAGLFGYFGPQMLGILRDQTGSFAAGFYMVAVADVITLSLIVVLY
                     RMTRGVTNT"
     gene            complement(1506..>1838)
                     /locus_tag="AC16CY_13120"
     CDS             complement(1506..>1838)
                     /locus_tag="AC16CY_13120"
                     /inference="COORDINATES: protein motif:HMM:NF017828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HIR3354781.1"
                     /translation="PDGYIRYGLDDPMAALASRTASFNEYLHNPPWAAEWIVHQAREH
                     RIDGALVLRPRSMKPAATGRLFIERALEDAGIPVLPIEADVVDARGWDADGARAGVRS
                     FLETRLLQ"
BASE COUNT          348 a          622 c          627 g          241 t
ORIGIN      
        1 tgaatcccag cggcgagacg acgatgtagc ggtgctgctc ggcgagtttc atcagcaggc
       61 cttcggcctg atccagatac gtgccctcgt tcgccccggc gccgtgcagc atcaggatga
      121 tcggcagcga cgccttccca tcccacgtct tcgggacgta gacgcgatac ggcatctgct
      181 gccccgtcgg cgcaaagaca tactgccggc gctggtcgcc ggtgccgcga gctggcggcg
      241 cctgggccag gatggtgagg ttcagcgcaa tcaccgtgca gacacacgtc acgaatgctc
      301 gactcatgtg ttggttactc ctcgcgtcat ccggtagagc actacgatga gagagagcgt
      361 gatgacgtcg gctacggcca ccatgtagaa gccggccgca aacgacccgg tctggtcgcg
      421 caggatgccg agcatctgag gaccgaagta tccgaacagg ccagcaccga ggctgacgac
      481 gcccatcgca tggcccgcct cgtggcgcgg catcaccagg ccggcaaggc cgtacagctg
      541 gttcgccgcg gtgaagccga agccgatcac cgccgccgtt gccacgcgga aaatcgccag
      601 gctctcgacg gccatcgacg cggaggcgac ggccagcagg atgaacacgg cgccgagcac
      661 cacgagcttg ttgaaccggt ccgccagcag gccgacgccg agattcaccg cgattgcggt
      721 gaggtaaccg gtgctgatga tgagtcccgc cgccgtcgca tcgagtccat acagcgcagc
      781 cgccacgctc gggacgaagt aggtcacggt gaattggccg aaaccgccga gcccgacgac
      841 cagcgcgagc atccacacga cgggcgagag gaaggcgctc ccgctgccgg cgccgtggcc
      901 agcgtggccg gcgctcgtcg ggtcatcgga cgtgggcctg tagaacagcc agaacacggt
      961 tgcgccgagc accgccatcg ccgcgtagcc gaggatggcg aggcgccagc cgaactcgcc
     1021 gacgagcaca cccccgagcg gagcgccgac gacagacgcc agcgccgatg tcgcgccgag
     1081 cacgcccatc gtacggccgc gcagcgatgg gggagcggtg agcgcgacgg cgatgagcac
     1141 gctgacgaac gcaaaccggt agccgaagat cgtcaggccg cggaacgcgg tcgcggaccc
     1201 gaagctccag gcatctccga gcagcacgct gcccgcggcc acgccgagca gcccgaagcc
     1261 gagcacgcgc ttctcgccga agcgcctggc gctgacgccg gccggcacgc tgagcagcat
     1321 ggccagcagg ccatacagcc cggtgaagaa tccgagctgc gtgtagctca tgccgaattc
     1381 ctgctgaatg gacggcagga gcggcgagaa ccccaggttc gacaactgca gcgtgaacat
     1441 ggcgatgagc gtcgccccga acggcaggag ccagttcgtc gagaccgccg gcagaacggt
     1501 cgtcgtcact gcagcagccg cgtctcgaga aacgagcgga cgccggcgcg cgcgccgtcc
     1561 gcatcccacc cacgcgcatc gacgacgtca gcttcgattg gcagaaccgg gatacctgcg
     1621 tcttcgagcg cacgctcgat gaagaggcgc ccggtagccg ccggcttcat gctgcgcgga
     1681 cgcaggacga gggcgccgtc gatccggtgc tcgcgtgcct ggtgcacgat ccactcggcg
     1741 gcccacggcg gattgtgcag gtactcgttg aagctcgcgg tgcggctggc cagcgcggcc
     1801 atcgggtcgt caaggccgta gcggatgtag ccgtcggg
//
LOCUS       DBNGSH010000571         8994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2094411, whole genome shotgun sequence.
ACCESSION   DBNGSH010000571 DBNGSH010000000
VERSION     DBNGSH010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2094411"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="AC16CY_13125"
     CDS             complement(<1..410)
                     /locus_tag="AC16CY_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354782.1"
                     /translation="MSAAGRRMGRVAVTLAVIVWALPTRAQTVPAPADSTRGVEFLPR
                     TLFHMTAERLSGDDPRLVWDAHLGGELDLVEYGSGRFTFVGDYEAILGEELRTFDPNQ
                     GSYILAAAASARARGTEFAAVFHHQSRHLSDRAK"
     gene            complement(407..1453)
                     /gene="lpxD"
                     /locus_tag="AC16CY_13130"
     CDS             complement(407..1453)
                     /gene="lpxD"
                     /locus_tag="AC16CY_13130"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR3354783.1"
                     /translation="MKLRDLADRLECRLDGDGDLDVTRVAGIQDAQPGDVTFLANPKY
                     EKALSTTHASAVILKEGAPAAPCAMLRAPDPYLAFARAVRLFNPESRPAPGVDRLAAV
                     ASDVRLGADVSIGPFAAIGEGVSIGDRTVIYANVTIGPGSRLGSDCVVHSNVAIRERC
                     SIGDRVILQNGVVVGSDGYGFARRGDGTHEKIPQVATVVIEDDVELGANSAVDRPAIG
                     ETRIKSGTKIDNLVHIAHGVTVGHNVLMAAQVGIAGSTDVEDDVVFGGQVGVVGHLTI
                     GRGAVLGAQAGVTNSIDAGAFVAGYPAIDTRVWRKGSVVFKRLPELKSRIERLEEQVA
                     ELTRALTQSERSSG"
     gene            1490..1789
                     /locus_tag="AC16CY_13135"
     CDS             1490..1789
                     /locus_tag="AC16CY_13135"
                     /inference="COORDINATES: protein motif:HMM:NF021193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HIR3354784.1"
                     /translation="MWIQEPIMFDVAYRLARRIVIAIVGATVLAVGIAMMVLPGPAFI
                     VIPIGLGILGLEFAWARRWLKAAKDKAEAALAMARSATSTGPGPGSAPPPGTSPQ"
     gene            complement(1800..2684)
                     /locus_tag="AC16CY_13140"
     CDS             complement(1800..2684)
                     /locus_tag="AC16CY_13140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C/P1 nuclease family protein"
                     /protein_id="HIR3354785.1"
                     /translation="MFRVIAFTSLAAVAASAPVSAWGYDAHKYIVERAIPLLPSALRP
                     LFEANRAVVVEHAIDPDLWRELFGDEEGPNHYLDYDAEGYGPYPFAALPRDYDEAVKK
                     FGKAHVDKYGRIPWRVEEIFERLKDSFAAYERRGSFGRYDILFYSSWLAHYVGDSHVP
                     FHAVVDSDGQRTGQRGIHTRFESLMFERYRGQLRVAPVAIAPIQNPRDFTFDALRVGT
                     TLVPGILDADRRAIGDRDIYDDRYYDAFFRSSQAVLERRLGEASAAVAAMIAGAWEAA
                     GKPAVPVSARDGVERRRR"
     gene            complement(2719..3537)
                     /locus_tag="AC16CY_13145"
     CDS             complement(2719..3537)
                     /locus_tag="AC16CY_13145"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR3354786.1"
                     /translation="MDVKALAQELTQAYAERRILPAPLSTREPGFDLEAAYAIESELT
                     RQRRSSGRTTVGRKVGYANKAMWRALKLETLVWAHMYDDTVTMAAQNTATVSLGKMCS
                     PKIEVEIVFKLRHPVDTANLDPAAILASVEWLALGFEIVDSVFAEWKFQPADFVAAFG
                     LHAALVVGEPRPVGADLIPSLAEQLPQCPLRLFKNGELVAQGSGRNSLRSPALCLGEL
                     ASAIARRAGEEPLAAGDLVSTGTLTEPQPILPGESWKASVDGIGLPDLTLVTGR"
     gene            complement(3563..4858)
                     /gene="oxlT"
                     /locus_tag="AC16CY_13150"
     CDS             complement(3563..4858)
                     /gene="oxlT"
                     /locus_tag="AC16CY_13150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0019531 - oxalate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA];
                     GO_process: GO:0019532 - oxalate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HIR3354787.1"
                     /translation="MSINNRWFQLCASVIAMIMIANLQYAWTLFVDPMQKQTGWLNSD
                     IQVAFSLFILFQTWVQPLDGWLVDRLGPRGFITAAGVLCGLGWAGMGYATSLPMLYTM
                     YCAAGIGAAFVYSCSIGSALKWFKERRGLASGIMAAGFGGGTALFIPTISRMIASDGY
                     QATFVATGLFQGLVILVVAQFLRHPPAQAQAAPSGAFRAGQPVRPGSAGGSVSPAPVS
                     PARAEARQYTTFEVLRTPRFYWMYAAFVLMATGGLLVTANAGPMSRAWGFEPAVLTLA
                     ATLSPLANGGARIFWGWASDYLGRENTMILTFVLQAVCLFMVIALGQTSAAWFVATLV
                     AVYFTWGQIYALFPATSGDYFGSRNATSNYAVLYTAKGVAAVIGGWFAALLAERSGSW
                     TLAFYGSALMALAAAGIAVYLRSVSAAAKARAARIPATA"
     gene            complement(4855..5862)
                     /locus_tag="AC16CY_13155"
     CDS             complement(4855..5862)
                     /locus_tag="AC16CY_13155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3354788.1"
                     /translation="MRIVIAGAGAIGGYIGARLARAGADVVLFARGPHLRAMQARGLR
                     VTSAEGDSFDVRPQTTGDLAAIGKADVVFLGVKAHSLTSLVPQLPPLFGPDTVVVSTQ
                     NGIPWWYFQSYGGELDGLRLERVDPGGVIASTIEPRRILGSLAYFATDLAEPGVIHHT
                     EGNRISFGEPDGTKSERAKSIAEALIAAGFRCPVTPRFRHEIWVKLLGNVAFNPISAL
                     TGGTLEELVRHPDTSRLVRELMIETETVAGKLGIELPISIDQRMAGAEKVGAHKTSML
                     QDYEAGRPMELEAVVGAVVELGDRLGVPMPCTRAVYACAKLLDEHRARRVMPKAEGVA
                     S"
     gene            complement(5859..7376)
                     /locus_tag="AC16CY_13160"
     CDS             complement(5859..7376)
                     /locus_tag="AC16CY_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HIR3354789.1"
                     /translation="MTADATPTTLIGLLSHVPDDLTAIIQPEQNVRVSYGDLKRQVQA
                     VAESLAAAGVGRGDRVGIALPNGLPNIVTFLAASVAGTAAPLNPGYKEEEFRFYLEDT
                     NAKVLVLPPDGLDEARRAAGDKVPVLTVDMDAAGTVKLSGVTGTRAVAPPSVDDVALI
                     LHTSGSTGRPKRVPLTHANLSISSRNVARSYTLSKDDVSLCVMPLFHVHGLVASTLAT
                     LATGGTVVVPQKFSPLSFWRVARDHGATWYSAVPTIHQLLLARADKGGARPAGAEKLR
                     FIRSCSAALPPQVMQDLETAFGAPVLEAYGMTEAAHQMASNPLPPAARLPGSVGRGTD
                     VRISIMDETGKHLASGERGEVVIQGPNVVSGYENNPEANATSFTSGWFRTGDQGFLDE
                     NGYLTLTGRLKEMINRGGEKISPREIDEVLLTHPNIAEAVCFGTPHATWGEEVAAAVV
                     VREATTEQDLLAFCKERLADYKRPKQIHIVDAIPRTATGKIQRRVVAQAFASQAS"
     gene            complement(7513..>8994)
                     /locus_tag="AC16CY_13165"
     CDS             complement(7513..>8994)
                     /locus_tag="AC16CY_13165"
                     /inference="COORDINATES: protein motif:HMM:NF015980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3354790.1"
                     /translation="RTRRVPLLVVAGVALTAAVALGGWLWSTRGGTGEAIDSVAVLPF
                     INDSGNTELDYLSDGLGESISNSLARIETLRVVPRSLVTKYKNQPVDFQTLARDLGVR
                     AVVTGRIVQRGDRLAVQAELIDAVAVAQIWGEQFERSALDVLGLQRDISTAIADNLRP
                     RLSDEEERRLTSGGTESVEAYQAYLRGRFEWNKRTREGLEAATSYFNEAIRIDPKYAL
                     AHAGLAYVHIVQAFYGYVPAGEGLREAQRAATNAVKLDDRSAEAHAALGWTSLRYEWD
                     WPKGQREFDRASALGAENATVFNWSAGVPLSRGQFDDAVAAARRAERLDPLSPGFYIG
                     MGYLMTYARRYNEAIDALTAALRIQPELGAAHRYLASVYRLTGRLDMAIAADQKAIQL
                     GDPHGTVDLATSLAAAGLTAEANQQLGPVIAHARQVRDGAFYIALAYTTLGRIDEAFA
                     WLEEAYSNRDPWLPFLAVHPEFDRLHGDPRFDALVRRIGIPIR"
BASE COUNT         1472 a         3056 c         3029 g         1437 t
ORIGIN      
        1 tccttggcac ggtccgacag gtgacgcgac tggtggtgaa agacagcggc gaattccgtg
       61 cctcgggcgc gcgcggatgc cgccgccgcg agtatgtagc ttccctggtt cggatcgaac
      121 gtgcgaagct cttcgcccag gatcgcctcg tagtccccga cgaaggtaaa ccggcccgag
      181 ccgtactcga ccaggtccag ctcgccgccg aggtgtgcat cccacaccag acgcgggtcg
      241 tcgccggaca gacgctccgc cgtcatgtga aacagcgttc gcggcaggaa ctcgacgccc
      301 cgggtcgagt cagccggagc ggggacggtt tgcgcccgtg tggggagcgc ccacacgatg
      361 acggccagcg tcacggcgac gcggcccatc cgtcggccgg ccgcgctcac ccagaggacc
      421 tttccgattg ggtcagcgcg cgcgtcaact cggcaacctg ctcctcgagc cgctcgatgc
      481 gcgacttcag ctcggggaga cgcttgaaga cgaccgaacc cttccgccac acgcgggtgt
      541 cgatcgcggg gtagccagca acgaacgcgc cggcgtcgat cgagttggtc acgcccgcct
      601 gtgcgccgag cacggccccg cggccgatcg tcaggtgtcc gacgaccccc acctgccccc
      661 cgaacacgac gtcgtcttcg acgtcggtgc tgccggcaat acccacctgc gcggccatca
      721 gcacgttgtg accgacggtg acgccatgag cgatgtggac caggttgtcg atcttggtgc
      781 ccgacttgat ccgcgtctcg ccgatcgccg ggcgatcgac ggcgctgttg gcgccaagct
      841 ccacgtcgtc ttcgatgacg acggtggcga cctgcgggat cttctcgtgc gtgccgtcgc
      901 cgcggcgggc aaacccgtag ccgtcgctgc cgacgacgac gccgttctgc aggatgacgc
      961 ggtcgccgat cgagcagcgc tcgcggatcg ccacgttcga gtggacgacg cagtcgctgc
     1021 ccaggcgcga gccgggaccg atcgtcacgt ttgcgtagat caccgtgcgg tcgccgatgc
     1081 tgaccccctc gccgatggca gcgaacggtc cgatcgacac atctgcgccc agccggacgt
     1141 ccgacgcgac cgccgccagg cgatcgaccc cgggcgcggg gcgcgactcg gggttgaaga
     1201 gacggacggc cctggcaaac gcgagatagg ggtcgggcgc gcgcagcatc gcgcacgggg
     1261 ccgcgggcgc accctccttc aggatgacgg ccgacgcgtg cgtggtactc agcgccttct
     1321 cgtatttcgg gttggccagg aacgtgacgt cgcccggctg cgcgtcctga atgccggcga
     1381 cgcgagtcac gtcgagatcg ccgtcgccgt cgagccggca ctccagacgg tccgctagat
     1441 cgcgcagttt cacagcaggg acgattgtaa tgcgtcccga tatgggatca tgtggatcca
     1501 ggaaccgatc atgttcgacg tcgcctatcg tctagctcgg cgcatcgtca tcgctatcgt
     1561 cggcgcgacc gtgctcgccg tcgggatcgc gatgatggta ctgcccgggc cggccttcat
     1621 cgtgattccg atcggcctcg ggattctcgg tctcgagttc gcatgggcgc ggcggtggct
     1681 caaggccgcc aaggacaagg ccgaggcagc cttggccatg gcgcgctcgg ccacgtccac
     1741 cgggcctgga cccgggtcgg cgccgcctcc aggcaccagc ccacagtaaa gggtatgcac
     1801 taccgccggc ggcgctctac cccgtcgcgc gcgctgacgg gcacggcggg ctttcctgct
     1861 gcctcccagg cgccggcaat catggcggcg accgccgcgc tcgcttcgcc gaggcgccgc
     1921 tcgagcacag cctggctcga cctgaagaac gcgtcgtaat accggtcgtc gtagatatca
     1981 cggtcgccga tggcccgccg atcggcgtcg agaatcccgg gcacgagcgt cgtccccacg
     2041 cgaagcgcgt cgaacgtgaa gtcgcgcgga ttttgtatcg gcgcgattgc gaccggggcc
     2101 acccgaagct gaccgcggta gcgctcgaac atcagcgact cgaagcgcgt gtgaatcccc
     2161 cgctgccccg tccgctggcc gtcggaatcg accacggcgt gaaacggcac gtgcgaatcg
     2221 ccgacgtagt gcgcgagcca ggacgagtag aacaggatgt cgtagcggcc gaacgagcct
     2281 cgccgctcgt aggccgcaaa cgaatccttg agccgttcga agatctcctc gacgcgccac
     2341 ggaatccgtc cgtacttatc gacatgggcc ttgccgaatt tcttgacggc ttcgtcgtag
     2401 tcgcggggca gcgcggcaaa gggataggga ccgtagcctt cggcgtcgta gtcgaggtag
     2461 tggttggggc cctcctcgtc gccgaaaagc tcgcgccaca ggtccgggtc tatcgcgtgc
     2521 tcgacgacga ccgcgcggtt ggcctcgaac agcggccgaa gcgccgacgg cagcagaggg
     2581 atggcgcgct cgacgatgta cttgtgcgcg tcgtagcccc atgcggacac cggagctgac
     2641 gcggccacgg cggcgagact ggtgaaagcg attactcgga acatgagggg attatcacct
     2701 cgggctaccg gctaccagct accggcccgt gaccagggtg agatcaggca gcccaatgcc
     2761 gtcaacgctt gccttccagc tctcacctgg caggatcggc tgcggctcgg tcagcgttcc
     2821 agtgctcacg agatcgcccg cggccagcgg ctcctcgccc gcccgccggg caatcgccga
     2881 ggccagctcg cccaggcaga gcgcggggct tcgcagcgag ttgcggccgg atccctgcgc
     2941 gacgagctcg ccgttcttga agagacgcag aggacactgc ggcagctgct cggcgaggga
     3001 cgggatgaga tcggcgccga ccggtcgcgg ttcgccgacg accagggcgg cgtgcaggcc
     3061 gaacgccgcg acgaaatcgg ccggctggaa tttccactcc gcgaacacgc tgtccacgat
     3121 ctcgaaaccg agcgcgagcc attcgaccga cgcgaggatg gccgccggat cgaggttcgc
     3181 ggtatccacg ggatggcgca gcttgaagac gatctccacc tcgattttcg gggagcacat
     3241 ctttcccagc gacaccgtcg cggtattctg cgcggccatc gtcacggtgt cgtcgtacat
     3301 gtgcgcccag acgagcgttt cgagcttgag cgcgcgccac atcgccttgt tggcgtaacc
     3361 gaccttgcgg ccgacggtgg tccggccgct cgaccgccgc tgtcgcgtca gttccgactc
     3421 gatcgcgtag gccgcctcca ggtcgaagcc cggctcgcgt gtcgagagag gggccgggag
     3481 gatccgccgc tctgcgtacg cctgcgtcaa ctcctgcgcc agcgccttca cgtccatctg
     3541 gttggtggct cgtggatggt cgtcatgccg tagcagggat tcgagccgcg cgtgcttttg
     3601 ccgcggccga gacagagcgc aggtaaacgg cgatgccggc cgccgcgagc gccatcaggg
     3661 cgctgccgta gaaggcgagc gtccagctgc ccgatcgctc cgcgagcagc gcggcgaacc
     3721 agccgccgat gacggcggcg acgcctttcg ccgtgtagag cacggcgtag ttcgacgtgg
     3781 cgttgcggga accgaagtaa tcgccggagg tggccgggaa gagcgcgtag atctgccccc
     3841 aggtgaagta gaccgccacg agcgtcgcga caaaccacgc ggccgacgtc tgaccgaggg
     3901 cgataaccat gaacagacag acggcttgca ggacgaaggt caggatcatg gtgttctcgc
     3961 gcccgaggta gtccgatgcc cagccccaga aaatgcgtgc gccgccattt gccagcgggc
     4021 tgagcgtggc ggccagcgtc aggacggccg gttcgaatcc ccacgcgcgg gacatcggtc
     4081 ccgcgttggc cgtgacgaga agaccgccgg tggccatcag gacgaatgcg gcatacatcc
     4141 agtagaaccg gggcgttcgc agcacctcga atgtcgtgta ctgacgcgcc tccgcgcgcg
     4201 ccggcgacac cggcgccgga ctcacgctgc ccccggcgct gccgggacga accggctgcc
     4261 ccgcgcgaaa tgcgccgctc ggagccgcct gcgcctgcgc gggtggatgc cgcaggaact
     4321 gcgccacaac gaggatgacg agcccctgga agaggccggt cgcgacaaag gtggcctggt
     4381 acccgtcgga ggcgatcatt ctcgagatgg tcggaatgaa gagcgccgtg ccgccgccga
     4441 agcccgccgc catgatgccg gaggcgaggc cccggcgctc cttgaaccac ttcagcgcgg
     4501 aaccgatcga gcagctgtag acgaaggccg ctccgatgcc ggcggcgcag tacatcgtgt
     4561 aaagcatcgg gagcgacgtc gcgtatccca tcccggccca gccgagaccg cacagcacgc
     4621 ccgcggccgt gatgaacccg cgcggaccga gccggtccac gagccagccg tcgagcggct
     4681 gcacccacgt ctggaagagg atgaagagcg agaaggccac ttgaatgtcg gagttcagcc
     4741 agccggtctg cttctgcatc ggatcgacga agagcgtcca ggcgtactgc aggttcgcaa
     4801 tcatgatcat cgcgatgacc gaggcgcaca gctggaacca gcgattgttg atgctcacga
     4861 tgcgaccccc tctgctttcg gcatgacgcg acgtgcccgg tgctcgtcga gcagcttcgc
     4921 gcaggcgtag accgcgcgcg tgcacggcat cggcacgccg agccgatcac ccagctccac
     4981 gaccgcgccc accacggcct cgagctccat cgggcggccg gcctcgtagt cctgcagcat
     5041 cgacgtcttg tgcgcgccga ctttctcggc cccggccatt cgctggtcga tcgagatcgg
     5101 cagctcgatg ccgagcttcc ccgccacggt ctcggtttcg atcatcagct cccgtacgag
     5161 gcggctcgtg tcgggatggc gaacgagctc ctcgagcgtg cctccggtca gcgcactgat
     5221 cgggttgaac gcgacgttgc cgagcagctt cacccagatc tcgtgacgga agcgcggagt
     5281 gaccggacac cggaagccgg cggcgatgag cgcctcggcg atcgacttgg cccgttcgga
     5341 cttggtcccg tcgggctctc cgaagctgat gcgattgcct tcggtgtgat gaatcacgcc
     5401 cggctcggcc aggtcggtgg cgaagtaggc aagggacccg agaatccgcc gcggctcgat
     5461 cgtcgaggcg atcacgcctc ccggatcgac gcgctcgagc cgcagaccgt cgagctcgcc
     5521 cccgtagctc tggaagtacc accacgggat gccgttctgc gtgctcacga cgacggtgtc
     5581 gggaccgaac aggggcggca gctgcggcac gagcgacgtg aggctgtggg ccttgacacc
     5641 gaggaacacg acgtcagcct ttccgatcgc cgcaagatcg cccgtcgtct gcggcctgac
     5701 gtcgaagctg tcgccctccg cgctcgtgac acgcagcccg cgggcctgca tggcccgcag
     5761 gtgcggaccg cgcgcgaaca gcacgacgtc cgcgcccgcc cgcgcgagcc gcgcgccgat
     5821 gtagccgccg atcgctccgg cgccggcgat gacgatcctc acgaagcctg cgatgcgaac
     5881 gcctgcgcca cgacgcgacg ctggatcttg ccggtggccg tccgcggaat ggcgtccacg
     5941 atgtggatct gcttcggccg cttgtagtcg gccagccgct ccttgcagaa cgcaaggaga
     6001 tcctgctcgg tcgtcgcttc tctcacgacg accgcggcgg cgacctcttc tccccacgtc
     6061 gcgtggggcg tgccgaagca gaccgcttcc gcgatgttgg gatgggtgag cagcacctcg
     6121 tcgatctcgc gcggcgagat cttctcgccg cccctgttga tcatttcctt cagccgcccg
     6181 gtcagcgtca ggtagccgtt ctcgtcgagg aacccctggt ctcctgttct gaaccagccg
     6241 ctcgtgaacg acgtggcgtt ggcttcgggg ttgttctcgt agccgctgac cacgttgggg
     6301 ccctgaatga cgacctctcc ccgttcgcca gaggccaggt gtttccctgt ctcgtccatg
     6361 atgctgatgc gcacatcggt tccccggccg accgatcccg gaagacgcgc agcgggcggc
     6421 agcgggttcg acgccatctg atgcgccgcc tccgtcatgc cgtaagcctc gagcaccggc
     6481 gcgccgaacg ccgtctccag atcctgcatc acctgcggtg gaagcgccgc gctgcaggat
     6541 cggatgaacc gcagcttctc ggcgcccgcc ggcctcgctc cccctttgtc cgcgcgagcc
     6601 aggagcagtt gatggatcgt cggcacggcc gaataccacg tggcgccgtg atcgcgggcg
     6661 acgcgccaga acgagagcgg gctgaacttc tgaggcacga ccacagtgcc accggtcgcg
     6721 agcgtcgcga gcgtggacgc cacgaggccg tgcacgtgaa acagcggcat cacgcacagc
     6781 gacacgtcgt ctttcgagag cgtgtagctt cgcgccacgt tgcgcgagga gatcgacaga
     6841 ttggcgtgcg ttagcggcac gcgcttgggg cggcccgtgc tgccgctcgt atgaaggatc
     6901 aacgccacat cgtccaccga cggcggcgca accgcacgcg tgccggtcac gcccgacagc
     6961 ttcaccgtgc cggcggcgtc catgtcgacc gtcaggacgg gcaccttgtc gccggccgcg
     7021 cgcctggcct cgtcgagacc gtcaggcggg agcaccagca ccttcgcgtt ggtgtcctcg
     7081 agatagaagc ggaactcctc ttccttgtat ccgggattga gcggagcggc ggtcccggca
     7141 acggacgcgg cgagaaacgt gacgatgttc gggaggccgt tcgggagcgc gatgccgacg
     7201 cgatcccccc gcccgacacc cgcggcggcg agcgactccg ccacggcctg aacctgccgt
     7261 ttgagatcac cgtaggagac gcggacgttc tgctcgggct ggatgatggc ggtcagatcg
     7321 tcgggaacgt ggctcagcaa tccgatcagc gtggtgggtg tggcatccgc agtcatggtc
     7381 gtgtctcctc acgtggtgtc cggcctttgc cggacccgtg gtgcgtcgta aggcggtaag
     7441 tctgccacga atggcctgat gctggcgacc gggacggcgc gcggctgtac tgatggaacg
     7501 cgcacccgcg actcagcgga tcgggatccc gattcggcgc acgagggcat cgaaccgggg
     7561 gtcgccgtgc agccgatcga attccggatg gaccgcgagg aagggcaacc acggatcccg
     7621 gttcgagtag gcctcctcca gccacgcaaa cgcctcgtcg atacggccga gcgtcgtgta
     7681 ggccagggcg atgtagaagg cgccgtcccg gacttgccgc gcgtgcgcga tgaccggccc
     7741 caactgctga ttggcttcag ctgtgagtcc ggccgctgcg aggctcgtgg ccaaatccac
     7801 ggttccgtgg ggatcgccca gctggatggc tttttgatcg gcggcgatgg ccatgtcgag
     7861 ccgtccagtc aatcgataca cggacgccag gtatctgtgg gcggcgccga gctcgggctg
     7921 gatccgcaga gccgcagtga gcgcgtcgat ggcctcgttg taccggcgcg cgtaggtcat
     7981 gaggtagccc ataccgatgt agaagccggg cgacagtgga tcgagccgct cggcgcgcct
     8041 ggcggccgca acggcgtcgt cgaactgccc tctcgagaga gggacgcccg ccgaccaatt
     8101 gaaaacggtc gcgttctccg cgccaagcgc cgatgcccgg tcgaactcac gttggccctt
     8161 tggccagtcc cactcgtaac gcaagctggt ccacccgagc gccgcgtggg cctcggccga
     8221 tcgatcgtcg agcttgacgg cattggtcgc ggcacgctgg gcctcgcgaa gcccctcgcc
     8281 cgccggcacg taaccgtaga acgcctgcac gatatgcacg tacgccagcc ctgcgtgggc
     8341 gagggcgtac ttcgggtcga ttcggatcgc ctcgttgaag tagctcgtgg cagcctcgag
     8401 gccctcgcgc gtgcgcttgt tccactcgaa gcgtcccctg agataagcct ggtaggcctc
     8461 gacgctctcc gtgccgccgc tggtcaggcg gcgttcctcc tcgtcactca ggcgggggcg
     8521 caggttgtcg gcgatcgcgg tggagatgtc gcgctgcagc cccagcacgt ccagcgcgct
     8581 tcgctcgaac tgctcgcccc agatctgcgc gacggcaacg gcatcgatga gctctgcctg
     8641 caccgcgagc cggtcgccgc gctgcacaat ccggccagtg acgacggctc tgacgcccag
     8701 gtcgcgggcg agcgtctgga agtcgaccgg ctgattcttg tactttgtga cgagggagcg
     8761 agggacgacg cgaagtgtct cgatgcgcgc gagactgttc gagatcgatt cccccagacc
     8821 atcgctcaga tagtcgagct ccgtgttgcc gctgtcgttg atgaacggca gcaccgccac
     8881 cgagtcgatc gcctccccgg tgccgcctcg ggtcgaccag agccaaccgc caagcgcgac
     8941 ggccgccgtc agtgccacgc ctgccacgac gagtaaaggt acacgtcgtg tgcg
//
LOCUS       DBNGSH010000572         7756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1585434, whole genome shotgun sequence.
ACCESSION   DBNGSH010000572 DBNGSH010000000
VERSION     DBNGSH010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1585434"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..1032)
                     /locus_tag="AC16CY_13170"
     CDS             complement(322..1032)
                     /locus_tag="AC16CY_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354791.1"
                     /translation="MLLLVAACAKSFEIPIETPIKPKLDTSPFQRVLVAGFIAGGTDD
                     VDGNLETTRLLRSQLRTKSQLRVIDSDALPLVEVANEGRDAPADAPPPPIKEEKDLEG
                     YEHVFANAEYWKRIGEEHQEPLIVTGTVLFTTMQRNGVVQQNREVYDSFGRRSVVPVR
                     MFVERKGFSLDGRFIFIDGRSGARLHTENFNEQILYNANQTTPALSSYFELMDRLIPA
                     FLSTLSSQKIKGTRFLLQ"
     gene            complement(1052..1393)
                     /locus_tag="AC16CY_13175"
     CDS             complement(1052..1393)
                     /locus_tag="AC16CY_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3354792.1"
                     /translation="MRIVVSTVALLVALAVAPLAADARSDAKQQVEFGILAAKRGLWR
                     EAIFRWERAAKIDPGYAAAHNNLAIAYELEGELMKARVAYERALELEPDNEMIKQNYE
                     LFREINDRQSR"
     gene            1738..2760
                     /gene="dprA"
                     /locus_tag="AC16CY_13180"
     CDS             1738..2760
                     /gene="dprA"
                     /locus_tag="AC16CY_13180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR3354793.1"
                     /translation="MTLDQAVALSLLADLSRVGLTERLKRCDPDLLVRAAPLLERAKQ
                     VRELGARQGIHAVPWNDPRFPAVLASLTDLPPVLWYQGHLESADAPSVAIVGSRMASA
                     AAKETAARLGADLAASGFTVVSGLARGVDSAAHRGALERGRTIAVLGSGLDRVYPAEH
                     VPLARQVAESGLVLSEFPPGTPPFPRHFPMRNRLISGLSLGVVVIEASEKSGSLITAS
                     CALEQGREVMAVPGSVLNGRNRGGHLLLRDGAKIVETADDIVEELGCGRRVVATGQGL
                     LSRYGSSTSADPVLRVMERGQAYDLDELACGSGLDLARLLPRLMDLELRGLVRRAGGG
                     RFLRAS"
     gene            2808..5525
                     /gene="topA"
                     /locus_tag="AC16CY_13185"
     CDS             2808..5525
                     /gene="topA"
                     /locus_tag="AC16CY_13185"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015147956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR3354794.1"
                     /translation="MAAKPLVIVESPAKAKTLGRFLGNKYRVEASYGHIRDLPESASE
                     VPKEIKAKEWGRLGVDVEQDFTPYYVVPTEKRKQVAHLKTAVKEASELLLATDPDREG
                     ESISWHLTQVLKPRIPVRRIVFHEITESAVKEALDNPAKVDENLVRAQESRRILDRLY
                     GYTLSPLLWKKVQTGLSAGRVQSVAVRLIVEREEERRAFRSAEYWDLEARLKGDGREF
                     VATLVRLNDQRVATGKDFDPQTGALKNQGVRLLDEAATSALVDAVRANVPWTVTSVEE
                     KPGVERPAPPFTTSTLTQEASRKLGFSTERTMQVAQRLFQGVDVGNDEMEGLITYHRT
                     DSTTLSDKALNESARVIRQMFGDEYYDGPRRYQTRVKNAQEAHEAIRPSDFRLAPSQL
                     EGVLDPDDLRVYELIWKRTMASQMVDARVLRTSLEISATGSKGDVAVLTASGRAIEFA
                     GFRRAYVEGSDDPAAELEEQEAILPQCRVGDLIHRDGSTPVTLLDAEPKRHETLPPAR
                     FTEASLIKELERLGIGRPSTYAPTIATIVRRGYVFRQNKALLPSFTAFAVTQLLREHF
                     GDFVETDFTAEMEDDLDEISRGERESVAFLKQFYYGDRKHRGLLPAVEKGAEKADYPV
                     LDLGVDSESGESVRVRIGRFGPFVQVADGGPGRTASLPEDVAPADLTVERAMELVRAK
                     AEGPRALGVDPATTQAVYVMNGRYGAYVQLGETPEAKGKGQKAQKPKRASLQSHMSES
                     TVTLEEALKLLSLPRVVGLHPDDNGEILTNLGRFGPYVKHGDEFRSLESEEDVFTISL
                     DAALALLRAPKQSRRRPSAQRKMLRELVENGTTIKVLEGRYGPYVTDGKTNASIPKTT
                     SPDALTYAQAADLLEARKGAVPSIRRARPRRRTVKAAAAQGSRRKAVGA"
     gene            5563..7002
                     /gene="trmFO"
                     /locus_tag="AC16CY_13190"
     CDS             5563..7002
                     /gene="trmFO"
                     /locus_tag="AC16CY_13190"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR3354795.1"
                     /translation="MIHIIGGGLAGSEAAWQAASLGVEVVLHEMRPVRPTAVHKTDRL
                     GELVCSNSFRADKLDNAVGLLKEEMRRLGSLVMRAADANRVPAGAALAVDRERFAQAL
                     TEAVHGHPLITVAREEVTSIPRDPSMYPLIIATGPLTSEALSDEIAALVGAGHLYFYD
                     AISPIVLAETLDMTKVFRASRWGRSLRGGSVATESPSRGGSLDPPQSDAPGVACSVDD
                     GEGDYLNCPLTGGEYDAFYDALATAESASVHDLDNERFFEGCLPIEVMARRGRDTLRF
                     GPMKPVGLVDPRTGRMPHAVVQLRQDNLAGDHFSLVGFQTQLKWGEQARVLRLIPGLE
                     RAEFVRFGLVHRNTYINGPTVLRETWQTKARPDLFFAGQISGVEGYVESAASGLIAGR
                     NAAALVRGAPPGIPPRTTAIGALAYYVSHATARDYQPTNITFGIMSPPDAPRGRRLTR
                     EERKLATSARALAALEEWIAGSLVPAAAR"
     gene            6999..>7756
                     /locus_tag="AC16CY_13195"
     CDS             6999..>7756
                     /locus_tag="AC16CY_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3354796.1"
                     /translation="MKSHLRDFVEYLRLNRNASAHTVAAYDGDLAQFLDFAARDLGKK
                     ELEPSDVQLATIRAFMADLYRRGHARASVARKLSALRAFGRYLRREGWIEADPATLAA
                     GPKRERKVPAHLSVDEMTRLLESPDTSGPLGRRDKAMLEVFYASGLRLSELVGLDLED
                     VNLRARMVRVMGKGAKERLVPFNSAAEQSLRSWLKDRAVLRSAAAPKARSGEPLFVNA
                     RGARLTGRSVQRLVARYVSSCCTRFGISPHALRHS"
BASE COUNT         1321 a         2528 c         2615 g         1292 t
ORIGIN      
        1 caccggcccc gacgacacca acccttagcc gtggctctgt ggcatctgcg ggatagcggt
       61 agtaccacgc ctttggccca tcgaccttga cggtcgcagt gctgacctct cggtcagcgg
      121 ccaacacgat tccgtccttg cacatgaacc cggcaacaac agtcatcgcg cgcttcctaa
      181 gaggaggccg ccttggagtg ttgcgcggaa tgcgccactg gagaggcggc gcgctagcat
      241 agcgtgtgcc gccggtgctt gctcgggcag gatcggcccg cgaaactaga ccattaccgg
      301 gcctcaccag gccaggacgt gctactgcag caggaagcgc gtgcccttga tcttctggct
      361 gctgagcgtg ctgagaaacg ccggaatcag ccggtccatc agctcgaaat aggacgagag
      421 cgccggcgtc gtctggttgg cgttgtacag gatctgttcg ttgaagtttt ctgtatggag
      481 cctcgcgccg cttcgtccgt cgatgaagat gaagcgaccg tcgaggctga accccttgcg
      541 ctcgacgaac atccggaccg gcaccacgct gcggcggccg aacgaatcgt acacttcgcg
      601 gttctgctgc acgacgccgt tgcgctgcat ggtcgtgaac agcacggtgc ccgtgacgat
      661 cagcggctcc tggtgttcct cgccgatccg cttccagtac tcggcgttgg cgaagacgtg
      721 ctcgtagcct tcgagatctt tctcttcctt gatcgggggc ggaggagcgt cggccggcgc
      781 gtcgcgcccc tcgttggcga cctcgaccag cggaagggca tcggagtcga tcacgcgaag
      841 ctgggacttg gtgcgcagct ggctgcggag cagccgcgtc gtctcgaggt taccgtccac
      901 gtcgtcggta cctccggcga tgaatccggc gacgaggacg cgctgaaagg gcgacgtatc
      961 cagttttggc ttgatcggcg tctcgatcgg gatctcgaac gacttcgcgc aggcggcaac
     1021 caggagaagc aacgcgagac taccgagcgt gctagcgact ctggcggtca ttgatttccc
     1081 tgaacagctc gtagttctgc ttgatcatct cgttgtcggg ctcgagctcc agcgcccgct
     1141 cgtaggccac gcgcgccttc atgagctcgc cctcgagctc atacgcaatc gcgaggttat
     1201 tgtgcgccgc cgcgtagccg ggatcaatct tcgccgcgcg ctcccaccgg aagatcgcct
     1261 ccctccacaa accacgcttc gcggcgagaa tgccgaactc gacctgctgt ttggcatcgg
     1321 accgcgcatc cgcggccagt ggagccacgg cgagagcgac gagcagcgcg acggtcgaaa
     1381 cgacgatgcg catgtcgcga gataacctca ctatagccca ctggttcctg caccgtgcaa
     1441 gtaacttgcc tgtcgcggaa tccaaattgc ggagcaaggc acgtaccacg ataaggcccg
     1501 tgaattcgtt ggcgaaaccg agactctggc tgagcaacag tcctgtgttt ggatcgatcc
     1561 tcgttgcgga ttcgtgggtc gctgcctata attcgtgaca tcgggctggg tgcaccaggc
     1621 agcgttgcga cgctcgcgtg ggccccggcc cttttccacg tacggggtcc gttttcgtca
     1681 tcgtcgcagc cctcctggct tggacgcgcg cgcgggcgcg ggctggagga actgttcatg
     1741 accctcgatc aggcagtcgc gctgtccctg ctcgcagacc tgtctcgagt ggggttgacg
     1801 gaacgactca agaggtgcga ccccgacctc ctggttcggg cggcccctct gctcgaacgc
     1861 gcgaagcagg tcagggagct cggcgcgcgt cagggcatac acgccgtgcc ctggaacgac
     1921 ccccggtttc cggccgttct cgcgtcgttg accgatcttc cgcccgtgct ctggtaccaa
     1981 ggacatctcg agtccgccga cgcgccgtcg gtcgcgatag tcggatcgcg aatggcttcg
     2041 gccgcggcga aggagacggc cgcgcgcctc ggagccgatc tcgccgcgtc aggatttacc
     2101 gtcgtcagcg ggctcgccag gggggtggac tccgccgcgc atcgcggcgc cctcgaacgg
     2161 ggccgcacga ttgccgtcct tgggtccggt ctcgaccggg tctatcccgc ggagcacgtg
     2221 ccgctggctc gtcaggtggc ggagtccggg ttggtgctga gcgagtttcc gcccggtacg
     2281 cctcccttcc cccgccactt cccgatgcga aacaggttga tcagcgggct gtcgctcggc
     2341 gtggtcgtga ttgaagcctc cgagaagtcg gggtcgctca tcacggcctc ctgcgccctc
     2401 gaacagggcc gcgaggtgat ggcagttccg ggaagcgtcc tcaacgggcg caaccgcggg
     2461 ggccatctcc ttctccggga cggagcaaag attgtcgaga ctgctgacga tatcgtagag
     2521 gagctgggct gtggacggcg ggtcgtcgcg accggtcagg gcctgttaag tcgctatggg
     2581 tcttcgactt cggccgaccc agtgctacgc gtgatggagc ggggtcaggc ctacgatctg
     2641 gacgagctgg cctgtggatc cgggctcgat ctcgcgcgcc tgctcccgcg gttgatggac
     2701 ctcgaactgc gcggactcgt gcgccgcgct gggggcggcc gtttcttgcg cgcctcctga
     2761 acgtgttagt gtcatcgctg ggaacaggga actggtaacg ctttgaaatg gctgcaaaac
     2821 cgctggttat tgtcgaatcg cccgccaagg cgaagacgct cggccgcttc ctcggtaaca
     2881 aataccgtgt cgaggcgagc tacggccaca tccgcgacct gcctgagtcg gcctccgaag
     2941 tgcccaagga aatcaaggca aaggagtggg gacggctggg ggtggacgtc gagcaggatt
     3001 tcacgcccta ctacgtcgtc ccgaccgaga agcgcaagca ggtggcccac ctgaaaactg
     3061 cggtcaagga ggcatcggag ctgctgcttg ccacggaccc ggaccgcgag ggcgagtcaa
     3121 tcagctggca cctgacacag gttctcaagc cgcggattcc ggttcgccgg atcgtgtttc
     3181 acgagatcac tgaaagcgcc gtcaaggaag cgctcgacaa tccggccaag gtggacgaga
     3241 acctcgttcg ggctcaggag agccggcgga ttctcgatcg gctgtacggc tacacgctgt
     3301 cgccgctgct gtggaagaaa gtgcagaccg gactgagcgc cgggcgtgta caaagcgtag
     3361 ccgtccgcct gatcgtggag cgcgaggagg agcggcgcgc gtttcgatca gcggagtact
     3421 gggacctcga ggccaggctg aagggtgacg ggcgcgagtt cgtcgccacg ctcgttcgac
     3481 tcaacgacca gcgggtcgcg accggaaagg acttcgaccc gcagaccggt gcgctcaaga
     3541 accagggcgt gcgactgctg gatgaagcgg cgacgagcgc gctcgttgac gccgttcgcg
     3601 ccaacgtgcc gtggacggtg acctccgtgg aggaaaagcc gggcgtcgag cggccggcac
     3661 cgcccttcac cacgtccacc ttgacgcagg aggcaagccg caaactcggc ttctccaccg
     3721 agagaacgat gcaggtggcg cagcgcctct tccagggcgt tgacgtcggc aacgacgaga
     3781 tggagggcct catcacgtat caccgcaccg actcgacgac gctgtccgac aaggcgctca
     3841 acgagtcggc gcgagtcatc cggcaaatgt tcggagacga gtactacgac gggccgcgcc
     3901 gttatcagac ccgcgtgaag aacgcccagg aagcccacga agcgattcgg ccgagcgact
     3961 tccgactggc gccgagccag ctcgagggcg tgctcgatcc ggacgacctc agggtctacg
     4021 agctgatctg gaagcgcacg atggcgtcgc agatggtcga tgcccgagtg ctcaggacca
     4081 gcctcgagat ctccgcgacg ggctccaagg gcgacgtcgc cgtgctgacc gccagcggca
     4141 gggcgatcga gttcgccggc ttccgccggg cgtatgtcga aggcagcgac gatcccgccg
     4201 cggagctcga agagcaggaa gccatcctgc cgcagtgccg ggtcggcgat ctgatacatc
     4261 gggacgggtc cactccagtg accctgctcg acgccgagcc gaagcgccac gagacgttgc
     4321 cgccggcgcg attcaccgag gcgtctctca tcaaggagct cgagcgcctc gggatcgggc
     4381 gcccgtccac ctatgccccg acgatcgcga ccatcgtcag gcgcggctac gtcttccgcc
     4441 agaacaaggc gctgctgccc agcttcacgg cgtttgccgt gacacagctg ctgcgagagc
     4501 acttcggcga cttcgtcgaa accgacttca cggcggagat ggaagacgat ctcgacgaga
     4561 tttcccgggg cgagcgcgag tcggtcgcgt tcctgaagca gttctattac ggcgaccgca
     4621 aacatcgagg tctgctcccg gctgtcgaga agggcgccga gaaggccgac tacccggtgc
     4681 tcgatcttgg cgtcgattcc gagagcggcg agtccgtgcg cgtgcgcatc ggccgcttcg
     4741 ggcctttcgt ccaggtcgct gatggaggtc ccggccgcac ggcgtcgctg ccggaggacg
     4801 tcgcgcccgc cgacctcacg gtcgaaagag cgatggagct cgtgcgggcg aaggcggaag
     4861 gtccgcgcgc gctcggcgtg gatccagcga cgactcaggc tgtctacgtc atgaacgggc
     4921 gctacggcgc ctacgtgcag ctcggcgaaa cgcccgaggc caaggggaag gggcagaagg
     4981 cccagaagcc gaagcgggcg tctctgcagt cgcacatgag cgagtcaacc gtgacgctcg
     5041 aggaagcgct caagctgctc tcgctgcccc gtgtcgtggg tctgcacccc gacgacaatg
     5101 gggagatcct gaccaacctc gggcggttcg gcccgtacgt caagcatggc gacgagttca
     5161 ggtcgctcga gtctgaggag gacgtcttca ccatctccct cgacgcggcg ctggcgctgc
     5221 ttcgtgcacc gaagcagtcg cggcggcgac cgtcggcgca gcggaagatg ctgcgcgagc
     5281 tcgtggagaa cgggacgacg atcaaggtgc tcgagggtcg atacggtcca tacgtgaccg
     5341 atggcaagac caacgcgtcg attccgaaga ccaccagccc tgacgctctg acctacgcgc
     5401 aggcggcgga tctgctcgaa gcccgcaagg gcgcggtccc ctcgattcgg cgggcccggc
     5461 cacgccggcg gacggtgaaa gcggccgcgg cacagggctc ccgccgaaaa gcagtcgggg
     5521 catagggcgt aagatcacag ggctcaggaa tcagggccct tcatgattca catcatcggc
     5581 ggaggccttg ccggcagcga ggctgcgtgg caggctgcat cgctcggcgt cgaagtggtg
     5641 ctgcacgaga tgcggccggt gcgccccaca gccgtgcaca agacggatcg gctcggcgag
     5701 ctggtctgct cgaactcatt ccgggccgac aagctcgata acgccgtcgg cctcctgaaa
     5761 gaggagatgc gacggctcgg ctcgctggtg atgcgggccg ccgatgcgaa ccgcgttccg
     5821 gcaggcgccg cgctggccgt cgatcgtgag cgcttcgcgc aggcgctgac cgaggctgtt
     5881 cacggccatc cgctcatcac tgtcgcccgc gaggaagtga cctcgattcc ccgcgacccg
     5941 tcgatgtatc cgctgatcat cgcgacgggc ccgctgacct ccgaggcgct gtcggacgag
     6001 atcgccgcgc tcgtcggcgc cgggcacctg tatttctacg atgcgatcag cccaatcgtc
     6061 ctggccgaga cgctcgacat gacgaaagtg tttcgcgcgt cacgctgggg gcgcagcctt
     6121 cgcggcggca gcgtcgcgac cgagtctccc agtagaggcg ggtctttaga cccgcctcag
     6181 tcagacgcgc ccggcgttgc ctgcagcgtg gacgatggcg aaggggacta tctgaattgt
     6241 ccgttgaccg gcggcgagta cgacgcgttc tacgacgcgc tggcgactgc cgaatcggcc
     6301 agcgtgcacg acctcgacaa cgagagattt ttcgaagggt gcctgccgat cgaggtgatg
     6361 gcgcgcaggg gccgggacac gctgcgcttc ggtcccatga agccggttgg tctcgtcgat
     6421 ccgcggaccg gtcgcatgcc gcacgccgtg gtccagctca gacaagacaa cctggccggc
     6481 gaccacttca gcctcgtcgg ctttcagacg cagttgaagt ggggagaaca ggcgcgcgtg
     6541 ctgaggctga ttcccgggct cgagcgcgcg gagttcgtcc ggttcggcct ggtgcacaga
     6601 aacacctaca tcaacgggcc aacggtgctg cgggaaacgt ggcagacgaa ggctcgcccc
     6661 gacctgttct tcgccggcca gatctcgggc gtcgaaggat acgtggagtc ggccgcctcc
     6721 ggcctcatcg ccggccggaa cgccgccgcg ctcgtccgtg gagcgccgcc gggaattccc
     6781 ccgcggacga cggccatcgg ggcgctcgcg tactacgtat cgcacgcgac cgcccgcgac
     6841 taccagccca cgaacatcac cttcggcatc atgtcgccgc ccgacgctcc ccgcggacgt
     6901 cgtctcaccc gcgaggagcg caagctggcg acctcggcgc gcgcgctcgc ggcgctcgag
     6961 gagtggatcg cgggctcgct ggttccggcg gcggcgcgat gaaatcgcac ctccgggact
     7021 tcgtcgagta cctccgcctg aatcgcaacg cctcggcgca taccgtcgcc gcgtacgacg
     7081 gcgacctcgc gcagttcctc gacttcgcgg cgcgagacct cggcaagaaa gagctcgagc
     7141 cgagcgacgt gcagctcgcc acgatacggg cgttcatggc cgatctctac cgccgcggtc
     7201 acgcgcgcgc gtcggtggcg cgcaagctgt ccgccctccg ggcctttggc cgctatctgc
     7261 gccgcgaagg ctggatcgag gcggatccgg caacgctcgc cgccggaccg aagcgcgagc
     7321 ggaaggtgcc ggcgcatctc tcggttgacg agatgacccg tctgctcgag tcgccggaca
     7381 cgtccggccc gctcgggcgg cgcgacaagg ccatgctgga agtgttctac gcgtccgggc
     7441 tgcggctgag cgagctggtg ggcctggatc tcgaggacgt caacctccgc gcgcggatgg
     7501 tccgcgtgat gggcaagggc gccaaggaaa ggctggtccc gttcaattca gccgccgagc
     7561 agtcgctgcg ctcctggctc aaggatcgag cggtgcttcg atcggccgcc gcgccgaaag
     7621 cccgcagcgg cgagccgctg ttcgtcaacg cgcgcggcgc gcggctcacg gggcggagcg
     7681 tgcagcgcct cgtcgcacgc tacgtctcgt cctgctgcac gcgcttcggc atcagcccgc
     7741 acgcgctgcg gcattc
//
LOCUS       DBNGSH010000573         7945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_736698, whole genome shotgun sequence.
ACCESSION   DBNGSH010000573 DBNGSH010000000
VERSION     DBNGSH010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_736698"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="AC16CY_13200"
     CDS             <1..993
                     /locus_tag="AC16CY_13200"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR3354797.1"
                     /translation="TDDAGLWRYKDGAAVYAHRLKQFTTTNMTAAEIHELGLREVKRI
                     EGDMDAILRTLGRTGGTVKQRMEKLAADLSYPNTSEGRAGIMADIDAMIRDADKRSSA
                     LFDLRPKSPVIAQPYPEFRWASAAASYTAPPLDGSRPGIFQMPLRPSSLTKASLRTLV
                     YHETIPGHHTQVAFGVENRQLPKFRQVRALGGISAISEGWALYAERMVAQEGWYEGDL
                     EGLLGQLDDELFRARRLVVDTGLHAMRWTRQQAIDFGIEASEVERYVVNPGQACSYKI
                     GQLEIFRLRDKARAALGQRYAIKEFHNVVLSAGSVPLTVLEGEVDRYIARKKAA"
     gene            996..2783
                     /locus_tag="AC16CY_13205"
     CDS             996..2783
                     /locus_tag="AC16CY_13205"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR3354798.1"
                     /translation="MRFQRLLPMLVFACAVPFVAAQSLRIDDAALGRAGQTGDDWLTY
                     GLNQSETRFSPLKEIDSTNVSRLGLAWSFDLKSGGGGQEATPLVANGVIYAVTNWSVV
                     MAVDARTGREKWRWDPWVNQTAVRPEICCGVVNRGLAIYQGMVFVPVIDGRLQALDAE
                     TGKVIWEARVAFPQDHYTLTMAPRIAKGKVFIGASGGDRPTRGFFDAYDAMTGRRAWR
                     FWTVPGDPSKGFENAAMKKAAETWDREWWKNGGGGAVWDGFAYDAEAGLVYVGTGNAQ
                     PWAWQHRSSRDKDNLYAASILAVDADSGELKWHYQVVPGDNWDFDSVQHLVLAELTIE
                     GRRRKVIMQANKNAFYYVIDRITGQFISAGPFSQITWARGIDPKTGRPIVNEEAFYGT
                     DAILISPGGGGAHNWAPMSFSPLTGLAYIPTSTANSFSYAAEPTFNPRPGGTTGTVRP
                     APTPVRPPPPAIGPPPIEGPAGRGALVAWDPVAQQMRWRMPGGGGIGGGTLATAGNLV
                     FQVLRDGRLVAYSADTGVKLHEIDTGLRSGMGPPITYRIDGKQFVAVMGGVGTVTAGS
                     AGPGNQATPFSPKLMTFVLDGSAMPSSAQ"
     gene            complement(2790..3824)
                     /locus_tag="AC16CY_13210"
     CDS             complement(2790..3824)
                     /locus_tag="AC16CY_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR3354799.1"
                     /translation="MTSRRAQFSQAVLTALRVAIVCTVLLPLQAWSQGSQPLRLVSTV
                     WSPFTNAAGEARFALDLVEEALKRIGVTSDTTFVQDADFTSSLMTGRADGSAAAWKDA
                     ERERVLLFSDPYLENRLILVGRRGSDVTATTLAALAGKRLVIVGGYSYGDAVKNGSGP
                     TFVQSSGEEDSLAKLLAGEADYTLMDELVVQYLVRNYPQQTRERLQLGTTPLIRRSLH
                     LAIRRTRTDAISVIARFNAELRRMIADRTYHRLLHVDWLRADIDGDGRPELISRTDQA
                     GLAPPTSSYDLFLSSPSTSPAPKPTGPRFYFGGNIYEDWASVPKNYKVPESNWNQPGR
                     STASIFRFTW"
     gene            complement(3891..5180)
                     /locus_tag="AC16CY_13215"
     CDS             complement(3891..5180)
                     /locus_tag="AC16CY_13215"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HIR3354800.1"
                     /translation="MSRAVSLEGFDTAFEMSAAAVRFGVGVTREVGMDLADRGARRAL
                     VVTDPVIATLPPLRAVVDALDANGVSAIVYDRVRVEPTEESWLDAIAFAGTQEFEAIV
                     AVGGGSSIDTAKAVNLYTTHPPADFLDYVNPPIGRGRAVPGPLKPLYAIPTTAGTGSE
                     TTGVAIFDLTRLHVKTGIASRLLRPTLGLLDPDHTRTLPPAVTASAGLDVLCHALESF
                     TAIAFTERPRPARPSVRPPYQGTNPISDVWALQALRMVSPHLVRAAEDPQDDEARASM
                     MLAASFAGIGFGNAGVHLPHAMAYPVAGGVKTYRAPGYPDHPLVPHGFSVILNAPAVF
                     RYTASASPERHLEAAAALGADVSRCRREDAGRALADRIAWFMDRLGVPMGLRAVGYSS
                     SDIPALVEGTLAQERLTKLAPRPADREALAAMFDESL"
     gene            complement(5276..5773)
                     /locus_tag="AC16CY_13220"
     CDS             complement(5276..5773)
                     /locus_tag="AC16CY_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354801.1"
                     /translation="MKLVAILCLAGGALAAVACADGPGIPTSPSATAGAAGASLAAAP
                     RSGDLHVTKECTEYTGLAGSFCTITSSNVKAIEVGSRVVYAQALGATTLDSDVVLDTP
                     GPGNNKANGHCALDLVTGLGLCTFDGGTGKFNGFTATAHVSPPTAADPENWHWDGTYS
                     FDPRD"
     gene            5969..6541
                     /locus_tag="AC16CY_13225"
     CDS             5969..6541
                     /locus_tag="AC16CY_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3354802.1"
                     /translation="MVSPRDVTVLLGEWSRGNRTALNQLLPLVYAELRRVAARQLRRE
                     RADHTLQPTALVHEAFIRLVDQRQGDWQNRAHFFGVSANVMRRILVDHARRHGASKRG
                     EGVRCVSIDGARDVAAPNEIPILALDQALDRLETVDAELARIVELRAFGGLTIEEAAH
                     VLSVSPSTAKRDWRTAKAWLTRELGAEVRS"
     gene            6538..>7945
                     /locus_tag="AC16CY_13230"
     CDS             6538..>7945
                     /locus_tag="AC16CY_13230"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3354803.1"
                     /translation="MSDERWRRVKALFQAAVERPAEERAAFLAAATGDDAALRREVES
                     LLRSDTSDTGFLDRLPVASASVLADPLGTTSMDPTRSHSFLAAGRRVGLYEIVAPLGA
                     GAMGEVYRARDTKLHRDVALKVLPERFALDPDRSARFTREAHLLATLNHPNIATIYGL
                     EESSTTQALVLELIDGPTLADLIAGASEDAPLPTRRGGSLDPPGRAIPVGQALAIAKQ
                     IADALEAAHEKGIIHRDLKPANIKIAGTGVVKVLDFGLAKVWDGGPQSDLLASQKLTV
                     TDLGGRTVLGTPAYMSPEQARGQPLDKRTDIWSFGCVLYEMLTGREPFAGGTVSDTLA
                     AILEHQPDLTRLPADTPVPIRRLLRRCLEKDRRKRLDSASDARLEIDDAIAFPAADTL
                     ASAATPSRRVMPAAIAALLVVIAALAAWILMRPAPPVPVPARFAIVPPPGLPLNVSSL
                     TRDLTLSPDGRSLVYRAGG"
BASE COUNT         1324 a         2675 c         2715 g         1231 t
ORIGIN      
        1 accgacgacg ccggcctgtg gagatacaag gacggcgcgg ccgtctacgc gcaccggttg
       61 aagcagttca cgacgacgaa catgaccgcc gccgagatcc acgagttggg gctgcgcgag
      121 gtgaagcgca tcgaaggcga catggacgcg atcctccgca cgctgggtcg caccgggggc
      181 accgtcaagc agcgaatgga gaagctggcc gccgacctca gttacccgaa cacgagcgag
      241 ggccgcgcgg gcatcatggc ggacatcgac gcgatgatcc gcgacgccga caagcggtcg
      301 agcgcactct tcgatctgcg tcccaagtcg ccggtcatcg cgcagcccta tcccgagttc
      361 cgctgggcga gcgcggcagc gagctacacg gcgccgccgc tcgacggctc gcggccgggg
      421 atcttccaga tgccgctgcg gccctcgagc ctgacgaagg cgagcctgcg cacgctcgtc
      481 taccacgaga cgattccagg gcatcacacg caggtggcgt tcggcgtcga gaaccgacag
      541 ctcccgaagt tccggcaggt gcgcgcgctc ggcggcatct ccgcgatctc cgaaggctgg
      601 gcgctctatg ccgaacggat ggtggcgcag gaaggctggt acgaaggcga cctcgaggga
      661 cttctcgggc aattggacga cgagttgttc agggcccgcc gcctggtcgt cgataccggg
      721 ttgcacgcga tgcggtggac gcgtcagcag gcgatcgact tcggcatcga ggccagcgaa
      781 gtcgagcgct acgtcgtgaa tcccggccag gcctgctcgt acaagatcgg ccagctcgag
      841 atcttcaggc tccgagacaa ggcgcgcgcg gcgctcggcc agcggtacgc catcaaggag
      901 ttccacaacg tggtgctgtc ggcaggctcc gtgccgttga cggtcctcga gggcgaggtt
      961 gacaggtaca tcgccaggaa gaaggcggca tagagatgcg attccagcgc ctgctgccga
     1021 tgctcgtttt tgcgtgcgcc gtgcccttcg tggccgccca gagcctgcgc atcgatgacg
     1081 ccgcgctcgg gcgcgccggc cagaccggcg acgactggct cacctacgga ctcaatcaat
     1141 cggagacgcg cttcagcccg ctcaaggaga tcgactcgac gaacgtgtcg cggctcggtc
     1201 tcgcctggtc gttcgatctc aagtcgggcg gcggcggaca ggaagccacg ccgctcgtcg
     1261 ccaacggcgt gatctacgcc gtcaccaact ggagcgtcgt catggccgtg gacgcgcgca
     1321 cgggacggga gaagtggcgg tgggatccgt gggtcaacca gacggcggtg cgcccggaga
     1381 tctgctgcgg cgtggtcaat cgcggcctgg cgatctatca gggcatggtc ttcgtgcccg
     1441 tcatcgacgg ccggctgcag gcgctcgacg ccgaaacggg caaggtgatc tgggaggcgc
     1501 gcgtcgcgtt cccgcaggat cactacacgc tgaccatggc gccgcgcatc gcgaagggga
     1561 aggtgttcat cggcgcgagc ggcggcgatc gaccgacgcg cgggttcttc gacgcctacg
     1621 acgcgatgac cggccggcgc gcgtggcgct tctggaccgt tcccggggat ccgtcgaagg
     1681 gcttcgagaa cgccgcgatg aaaaaggcgg cggagacctg ggaccgtgag tggtggaaga
     1741 acggcggcgg cggcgccgtg tgggacggct tcgcctacga cgccgaagcg ggactggtgt
     1801 acgtcggcac cggcaacgcg cagccgtggg cctggcagca ccgctcgtcg cgcgacaagg
     1861 acaacctgta cgccgcctcg attctcgccg tggacgccga cagcggcgag ctgaagtggc
     1921 actaccaggt cgtgcccggc gacaactggg acttcgacag cgtccagcac ctcgtcctcg
     1981 ccgagctgac gatcgagggc cgtcggcgca aggtgatcat gcaggccaac aagaacgcct
     2041 tctattacgt gatcgatcgc atcacgggac agttcatctc ggccgggccg ttctcgcaga
     2101 tcacgtgggc caggggcatc gatccgaaga ccgggcgccc gatcgtcaac gaggaagcgt
     2161 tctacgggac cgacgcgatt ctgatctccc ccggcggcgg cggcgcgcac aactgggcgc
     2221 cgatgtcgtt cagcccgctc accggcctgg cctacattcc tacgtcgacg gcgaactcgt
     2281 tctcatacgc ggccgagccg acgttcaatc cgcgcccggg cgggacgacg ggtacggtgc
     2341 ggcccgcgcc gacgccggtg cggccgccac ctcctgcaat cggaccgccg ccgatcgaag
     2401 gcccggctgg acgcggcgcc ctcgtcgcct gggaccccgt cgcgcagcag atgcgatggc
     2461 ggatgccggg tggcggcggc atcggcggcg gcacgctcgc cacggccggc aatctcgtct
     2521 ttcaggtgtt gcgcgacgga cggctggtcg cttacagcgc cgacacgggc gtcaagctgc
     2581 acgagatcga cacaggattg cggagcggga tgggaccgcc gatcacttac cggatcgacg
     2641 gcaagcagtt tgtcgcggtg atgggcggcg tcggcacggt gacagccggc agcgcgggac
     2701 cgggaaatca ggccactccc ttctcgccga agctgatgac gttcgtgctc gatgggagcg
     2761 cgatgccgtc atcggcgcag tagtgtcgat caccaggtga aacggaagat ggacgcggtc
     2821 gagcggccgg gttggttcca gttggattcg ggaaccttgt agttcttcgg caccgaggcc
     2881 cagtcttcgt agatgttgcc gccgaaatag aagcgcgggc cggttggctt cggcgccggc
     2941 gacgtcgagg gacttgacag gaacagatcg tagcttgacg tcggtggcgc aaggcctgcc
     3001 tgatccgtgc gggaaatcag ttccgggcgg ccgtccccgt cgatgtccgc gcgcagccag
     3061 tccacgtgca gcaggcggtg ataggtccgg tcggcaatca tgcggcgcag ttccgcgttg
     3121 aaacgggcga tgacagagat cgcatcggtc cgcgtgcgtc gaatggcgag atgcagcgag
     3181 cgcctgatga gcggtgtcgt acccagctgc aaacgttcgc gtgtctgctg cgggtagttc
     3241 cggacgagat actgaaccac gagttcgtcc atcaacgtgt agtccgcttc gccggcgaga
     3301 agcttcgcaa ggctgtcctc ttcgccgctc gactgcacga acgtcggccc ggatccgttc
     3361 ttcacggcgt cgccgtacga gtagccgccg acaatcacga gccgcttccc ggcgagtgcg
     3421 gccaacgtgg tcgccgtcac gtcgctgcca cgccggccga cgaggatcag ccggttctcg
     3481 aggtacgggt cggaaaacag cagcacgcgc tcgcgttcgg catccttcca tgccgcggcg
     3541 ctcccgtcgg cgcggccggt catgagcgat gacgtgaagt cggcgtcctg tacgaacgtg
     3601 gtgtccgacg tcacgccgat gcgcttcagg gcctcctcga ccaggtcgag agcgaagcgt
     3661 gcctcgccag cagcattcgt gaatggcgac cacacggtcg ataccaggcg cagtggctgc
     3721 gagccctggc tccacgcctg cagtggcagc agaacggtgc agacgatcgc gacgcgtaaa
     3781 gcggtcagaa ctgcctggga aaactgcgct cgccgcgaag tcatcacgaa gaacctctca
     3841 aggctcgatc atacttcgtg gccggccctt cgcgatcctc gtggctgagg tcagaggctc
     3901 tcgtcgaaca tcgcggccag cgcctcccga tccgcgggcc gcggcgccag cttcgtcagc
     3961 cgctcctgcg ccagcgtccc ttcgaccagc gccgggatgt cggacgagga gtaccccacg
     4021 gcgcggagcc ccatgggcac gcccagacga tccatgaacc acgcgatgcg gtccgcgagc
     4081 gcgcgtcccg cgtcctcccg gcggcagcgc gacacgtcgg cccccagtgc ggccgcagcc
     4141 tcgagatgcc gctcgggact ggctgaggcg gtataacgga acaccgcggg cgcgttgagg
     4201 atcaccgaga agccgtgcgg cacgagcgga tggtccggat agcccggcgc ccggtacgtt
     4261 ttgacgccgc ctgccacggg atacgccatc gcgtgcggca ggtgcacgcc ggcattgccg
     4321 aacccgatgc ctgcaaacga tgcggccagc atcatgctcg cgcgcgcctc atcgtcttgg
     4381 gggtcctccg ccgcacggac aagatgcggc gacaccatcc gcaacgcctg cagcgcccag
     4441 acgtcactga tcgggttggt gccttgatac ggcggacgca ccgaaggccg cgcaggccgt
     4501 ggccgctcgg taaaggcaat ggcggtgaac gactccagcg catgacagag gacgtcgagc
     4561 ccggccgacg cggtcacggc cggcggcagc gtgcgcgtgt gatcggggtc cagcagcccc
     4621 agcgtcggcc tgagcagccg gctcgcgatg ccggtcttca cgtgcagccg cgtcaggtcg
     4681 aagatcgcca cgccggtcgt ctcgctgccg gtgcccgcgg tcgtcgggat ggcatacaga
     4741 ggcttgagcg gccccggcac ggcccggccc cggccgatcg gcgggttcac gtagtccagg
     4801 aagtccgccg gcgggtgtgt cgtgtagagg ttcaccgctt tcgccgtgtc gatggacgac
     4861 ccgccgccga ccgcgacgat ggcctcgaac tcctgcgtcc cggcgaaggc gatcgcgtcc
     4921 agccacgatt cctccgtcgg ctccacgcgc acgcggtcgt acacgatcgc cgacacgcca
     4981 ttggcatcga gcgcgtcaac gacggctctc agcggcggca gcgtcgcgat gaccggatcg
     5041 gtgacgacga gcgcgcggcg ggcgccgcga tcggccaggt ccatgcccac ctcgcgcgtg
     5101 acgccaacgc cgaaacgtac ggccgccgcc gacatctcga atgcggtgtc gaaaccctcg
     5161 agtgagactg cccgggacat cgcgcgactg tagcccggac gtggccgctc acgccacggt
     5221 caactcgttg gcgcggtgat gaccgtggcg tcgggggaga gagacaattc ccggctcaat
     5281 cacgcggatc gaagctgtac gtcccgtccc agtgccagtt ttccgggtct gcagccgtgg
     5341 gcggtgagac atgggccgtc gcagtgaacc cgttgaactt cccggtcccg ccatcgaacg
     5401 tgcacagccc gaggccggtc accaagtcga gtgcgcaatg gccgttcgcc ttgttgttgc
     5461 cgggccccgg cgtgtcgagg acgacgtcac tgtccagcgt ggtagcacca agcgcctggg
     5521 catagacgac cctcgagccg acctcgatcg ccttgacgtt cgaggacgtg atcgtgcaga
     5581 aggaacccgc gaggcccgta tactccgtgc actccttggt cacatggaga tctccgcttc
     5641 gcggggccgc cgccaacgat gcgccggccg cacctgcagt cgcgcttgga cttgtcggaa
     5701 tcccgggtcc gtcggcacac gcgacggcgg ccagcgcccc accagcgaga cacagaatgg
     5761 ccacgagttt catacaccct ccttcggcgt tgccgcccgt tttcgtactg ccgcctgccc
     5821 ggcgcactga cgcgctcgag agcgccgtca ataggagatc gcgcaagcgg cggccgtaac
     5881 gggtcaaggc ggcagggaaa cctgtgatcg acgtctcccg gtgctatgct ttccccccga
     5941 ttcgactggg tcgagagagc aggctccgat ggtctccccg cgcgacgtca cagtgctgct
     6001 cggcgagtgg agtcgcggca accggacggc cctcaaccag ttgctcccac tcgtctatgc
     6061 ggagctccgc cgcgttgcgg cccgccagct tcggagggag cgggccgacc acaccctgca
     6121 gccgacggct ctcgtgcacg aggcgttcat ccggctcgtc gatcaacggc agggcgactg
     6181 gcagaatcgg gcgcatttct tcggcgtctc cgcgaacgtc atgcgccgca tcctggtcga
     6241 ccacgcccgt cgtcacggcg cgagcaagcg gggcgagggt gtgcgctgcg tgtcaatcga
     6301 tggagccaga gacgtcgccg cgcccaacga gatacccatc ctcgcgttgg atcaggcgct
     6361 cgatcgcctg gaaacggtcg acgctgaact ggccaggatc gtggagcttc gcgcgttcgg
     6421 cgggctcacg atcgaggaag cggcacacgt gctcagcgtg tccccctcga ccgccaaacg
     6481 cgactggcgc accgcgaaag cgtggctcac tcgtgagctc ggcgccgagg ttcgatcgtg
     6541 agcgatgagc gatggcggcg ggtgaaggcg ctgttccagg ccgccgtgga acggccggcc
     6601 gaggaacgcg ccgccttttt ggccgcggca accggggacg acgcggcgct gcgccgtgaa
     6661 gtcgagtcgc tgttacggtc ggacacctcg gacaccggct tcctcgatcg gctgcccgtc
     6721 gccagtgcgt ctgtgctggc cgatccgctc ggcacaacgt cgatggatcc cacgcggtca
     6781 cactcgtttc tcgcggcggg gcgtcgcgtc ggtctctacg aaatcgtcgc gccgctcggc
     6841 gcgggcgcca tgggcgaggt ataccgcgcg cgcgacacga agctgcatcg cgacgtcgcc
     6901 ctgaaggtac tgcccgagcg gttcgccctc gatccggatc gctcggcgcg gttcacgcgc
     6961 gaggcgcacc tgctggccac cctcaatcac ccgaacatcg ccaccatcta cggcctcgaa
     7021 gagtcgagca ccacgcaggc gctggtgttg gagctgatcg atggaccaac gctcgccgac
     7081 ctgattgccg gcgcgtctga agacgcgccg ctaccgacac gtagaggcgg gtctttagac
     7141 ccgcctggac gcgcgattcc ggtgggccag gcgctggcga tcgcgaaaca gatcgccgac
     7201 gcgctggagg cggcccacga gaagggcatc atccatcgcg acctcaagcc ggcgaacatc
     7261 aagatcgccg gtaccggagt ggtgaaggtg ctcgacttcg ggctggcgaa ggtctgggac
     7321 ggcggcccgc agtccgatct cttagcctcc cagaagctga ccgtcacgga tctcggcgga
     7381 cggaccgtcc tgggcacacc ggcctacatg agcccggaac aggcgcgggg ccagccgctg
     7441 gacaagcgga cggacatctg gtcgttcggc tgcgtgctct acgagatgct cacgggccgc
     7501 gagccgttcg ccggcggcac cgtctccgac accctcgctg cgattctcga acaccagccg
     7561 gacctgacga ggctgcccgc cgacacgccg gtaccaatcc ggagattact gcggcggtgt
     7621 ctggaaaagg atcgcaggaa acgactcgat tcggcgtccg atgctcgact ggaaatcgac
     7681 gacgcgatcg cgtttccagc cgccgacacg ctcgcatctg cggcgacgcc gtctcgtcgc
     7741 gtgatgccgg cggcgatcgc ggcgctcttg gtcgtcatcg ccgcgcttgc cgcttggatc
     7801 ctgatgcggc ccgcgccacc ggttccggtg ccggcgcggt tcgcgatcgt gccgccgccc
     7861 ggacttccgc tcaacgtgtc aagcctcact cgcgacctca ccttgtcgcc cgacggccgc
     7921 agcctcgtct accgcgccgg cgggt
//
LOCUS       DBNGSH010000574        13292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1585517, whole genome shotgun sequence.
ACCESSION   DBNGSH010000574 DBNGSH010000000
VERSION     DBNGSH010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 13292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13292
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1585517"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="AC16CY_13235"
     CDS             complement(<1..143)
                     /locus_tag="AC16CY_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIR3354804.1"
                     /translation="MTLQQLVGIELPIVQAPMAGVQGSALAVAVCNAGGLGSLPCAML
                     ALD"
     gene            complement(199..1320)
                     /locus_tag="AC16CY_13240"
     CDS             complement(199..1320)
                     /locus_tag="AC16CY_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3354805.1"
                     /translation="MRAGFILVFALLLAPSHTAQTPAPAAAPASEWKQFRGTPSLTGV
                     SSGTLPAALKVVWTYDARDLVDSSAAIVDGVVYVGGGNGDLLALDLATGKLRWKYTTG
                     NLIGESSPAVGGGVAYIGDLAGLLHAVNVHDGSKRWTFTTGSEIKSSPVIVGDVVLIG
                     SYDAHLYGLDAKSGQLKWKFETNGMVHATPAIHDGMAFIAGCDSVLRAIRVADGKEVY
                     QIEAGAYTGASPLITNNRAYFGTFNNEVLAFDLGRRRRLWRYAPKDRQFPFYSSAALG
                     SGRIVLGGRDKFVHALDIATGNPIWTFATRARVDSSPVIAGGRVYVGSGDGRLYVLDL
                     ASGKKLFEFDAGSGITTSPAIAGGRIVISSQDGIIYALG"
     gene            complement(1326..2783)
                     /locus_tag="AC16CY_13245"
     CDS             complement(1326..2783)
                     /locus_tag="AC16CY_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3354806.1"
                     /translation="MATFKRPRLHLVFLALLAALACALPTAYLSAADPGTNDWPMWGG
                     TPDRNLVSNMKNVPSEWDVKSKKNIKWVAELGSQSYGNPVVAGGMVFVGTNNELVRDP
                     KQPGDRGVLMAFRESTGEFLWQHTHAKLEAGRANDWPFQGVASSPAVEGERLYYVSNR
                     GVVLCLDIQGFRDKENDGPVTDEKFVGQADADVIWSFDMLEEVGSYPHNLSNSSPVVF
                     GNLIYVSTSNGQDESHVNIPSPRAPSIIALDKNTGKLAWEDNSVENRILHGQWSTPSV
                     GRIGGVLQVVSAQGDGWVRSYEAETGKKLWEFDTNPKDSVWPRTRNELIATPVIYQDH
                     VYIANGQDPEHGEGVGHMYAIDATKRGDITKTGLVWHFDKIRRSISTAAIADGLLYIA
                     DFSGYLHCLDVKTGQEYWVHDMLAAVWGSPFVIDSKVYLGDEDGDVTVLAHSKEKVVI
                     AEMNMGSAVYATVVPANGTLFLNNRSQLFAIGATK"
     gene            complement(2822..5044)
                     /locus_tag="AC16CY_13250"
     CDS             complement(2822..5044)
                     /locus_tag="AC16CY_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3354807.1"
                     /translation="MRKTCWLVATVVMALSVVLPAQGPRRVRVGDWPELRGPNRDGIS
                     RETGLISSWKLNGENFLWRVPSGGRSTPVVMGDRVYVQNPVGRGASLQERVMALDAAT
                     GKVIWEYHINLFQSDVPAHRIAWASPAVDPETGNIYVLSGGAEAIALSPDGKRLWSRS
                     FGEEFAAFTTHGGRTMSPVVDGDLVIVSAAVSNWGAAAARAHRLIALDKRTGDVIYVA
                     NPGGRPYDTAYAPPSIATINGMRLLIDGLGDGAVYAIQSQTGKKVWGFPVSKRAINTG
                     VAIRGNTVFLSHGDENLEGVELGLIAAIDGSQTGDIKTATWAIRGTEFGYSSPIIDGT
                     RLYQLDNASTLRAYDTATGKQIWTLPMGLAQKASPVLADGKIYVGTDGGTFFIVRPQA
                     DRGEILSQVELPNSTNSCCGSEGTPEQILGNAAISRGRIFFMSSDAIYAIGAKSATSP
                     TGLAVDEPAVAGSGAPAHLQVSPTELNLVPGQTVKLRARLFDAAGRFLREEKATWSLE
                     GLEGTIADGALTVANKPVGQAGVIKAAAGGLTGEARARVAHPLPWTETFDGYADGAIP
                     PGWANTVPGRLVVATLDGQKVLQKEPLNTIFQRGRTFIGPPDWSNYTFQADVRAPERR
                     RQMADVGITAQRYSLVLYGTAQQLKLEPWEPETVRTVTVPFQWKPDTWYRLKVRVENL
                     PNGQVRARGKAWAVGQAEPAAWMIDKTDPIGNREGAPGLFINAPFGAYLDNFELTRNQ
                     "
     gene            5242..7425
                     /locus_tag="AC16CY_13255"
     CDS             5242..7425
                     /locus_tag="AC16CY_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3354808.1"
                     /translation="MTHRRVASYPQQLAARATALSVASALLLTPSAARGQSTQSQTTQ
                     PAATFQLPTLIVTAQKEPADAQTLPLSLTAVPKTTLENAGVNIVSEAGAYGPNLHFTE
                     FTARKLSNARFRGIGGSPANPSVTTLYDGVPQLHANTASLDFLDVEQAEFVRGPQGTL
                     FGRNTLGGLVNITSIRPSLADWSGTFSVPFANNASRDVRGSINGPLADGKMGVALSMH
                     YGRRDGYTVNDLTGNDLDSRSGFAAKGQFLWTPTTAWETRVIVNGERDRDGDYALSDL
                     EGLRQNPFHTARDFEGHTNRDVLATTIVNRHEGQRVAVSSTTGFVKWDTEDATDLDYS
                     PLPLLTRNNVEESFQFTQEVRLSSAAKAPVRLGGDSRLAWQAGVFLFTQNYDQNAVNT
                     LGPIFTSLPFDVANTAPLAALDDFGVGVYGQGTATLATRLDLTAGVRVDYEQKDANLS
                     TFYTPALPFLPPQRDIVADESYANVSPQFAAAFHVQPGKTIYAAVANGYRAGGFNPSS
                     PVGSEAYGEEHTWSVEGGVKTAWAGERVTANASVFWIDWSDLQTNVPNPQVPGEFYIA
                     NIGGAASSGVEVEVNARVHPGVDVFGSVGYTHARFDEGSTSSGAPVGGNIIPNTPDYT
                     GTIGVQISHDVRQGVSLYGRAEGIFSGAFMYDDFNLEGQDAYSLANFRAGVRSRWVFV
                     EAWIRNAFDTRYIPTAFAYGPLSPSGFIGEMGNPRTFGVSAGVGF"
     gene            7482..8663
                     /locus_tag="AC16CY_13260"
     CDS             7482..8663
                     /locus_tag="AC16CY_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3354809.1"
                     /translation="MAQTPSTDWPQWRGPDRNGVSKETGLLKQWPKSGPALVWSASQL
                     GGGYGSVAVRGDRVYLQGMKNRQSIVTSLDRATGKFVWSAQLGSSGNNDRGSGPRSTP
                     TVDGDRVFVLTENGDLASLQAADGKIVWRRNILQEFRGRNINWLISESPLVDRDLVIV
                     SPGGRNAGMVALEKATGKTVWISKELSEEAGYASPVVADVQGVRTIMTLTSEAAVGVR
                     ASDGKLMWRYGNVANGTANITTPVYADGKVFFTSAYGTGGALLGLRAAGEENRAQEIY
                     FTSNMQNHHGGVVLVDGYLYGFSNSILTCMEFATGKVMWRDRSVGKGSVAYADGHLYI
                     LSENNVVGLVEASPTGYREKGRFPVPDQGWSSWAHPVVSGGRLYIRNQEILASYDVRA
                     R"
     gene            8755..9996
                     /locus_tag="AC16CY_13265"
     CDS             8755..9996
                     /locus_tag="AC16CY_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3354810.1"
                     /translation="MSSSSRISVALVVLAALVMPVSAQRASNDWTQWRGPSRDGAAPA
                     VADVSAWPEKLTQKWKVEVGQGYATPLVVGNRIYQFARKGEREVMMAIDADSGKVLWE
                     TGHTATFTMMPATKQHGPGPKSTPLFANGRLYSIGMTGIVTAYDAASGKQLWQKPGST
                     VVPMFTTHSFSPVIDRGLVVFHLGGHDEGALTAFDASTGDQRWSWKGDGPGYGSPVLA
                     DFGGTRQIVTITQGKLVGVDVATGALLWERPFVSPNNTNSNTPVVAGDLLIVSSNGPP
                     TSALRVSKQGDKWSVETVWENADVPMRLSDAVLAGDVLFGLSTRNSGQYFGVDVKTGK
                     TLWTSEGRQGGHAAIARAGNLLFSLEDDGELVIVQNSRTAFEPLRRYKVADVATWGQP
                     SYSGNRVFVKDETNLTLWTTK"
     gene            complement(10502..11338)
                     /locus_tag="AC16CY_13270"
     CDS             complement(10502..11338)
                     /locus_tag="AC16CY_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354811.1"
                     /translation="MTAIAIWLNDEEPKHPTLWIVADSRISGASAAPLIEDGAKIFSL
                     PVVCRAPGQDGFFSRISYTHSFGYCFAGSTLMGQNAYLALVPLLGNLAARADYVPSMA
                     DVASYVHRYLLHTFDDFKSKVGESAMFEVAIFGHCGRTNRLTAFHVEPGRDEAGTWTL
                     KCSEREDIQVGHPLYLGADKSRMMERFAAALGSAAPGRPLSRMPRYVIQDCISDESLP
                     SIGGDVQLGIANSFGFRALALVKPRVYGKPEAYISYLGRELTPDLAQLGEALVAGPAM
                     VG"
     gene            complement(11657..12499)
                     /locus_tag="AC16CY_13275"
     CDS             complement(11657..12499)
                     /locus_tag="AC16CY_13275"
                     /inference="COORDINATES: protein motif:HMM:NF039679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6188 family protein"
                     /protein_id="HIR3354812.1"
                     /translation="MPSSDLSWMIGARVRAISFVEPSSWHFEFVGGGTLRTETLWRII
                     SKGKVQATSVDHGHQFGLQQPVDAAALAKGSLAEATVRHASIARETGDVVVDFANGSR
                     LEVLTTSSSYEGWSIVSPRGDEVIGLGGGSIDVRRREANRPLHLTAAGPARRQFLVAA
                     GALIAADGVRVTAAQITGGTMFGLIGKITAVAGQREALASILIDGTGGMPGCLSYVVA
                     ADTADRDALWVTEVWEDQAKHQASLKLPAVQAAIAKGRPLIAGFSNRTETTPLGGYGI
                     PAAR"
     gene            complement(12731..>13292)
                     /locus_tag="AC16CY_13280"
     CDS             complement(12731..>13292)
                     /locus_tag="AC16CY_13280"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR3354813.1"
                     /translation="GFAVVMTRRDDSFIPLEERTAIANREGADLFLSIHANASTDTAA
                     RGIETYFLNLTTNPAAEAVAARENASSGRGMGQLPDIVKAIALNNKIDESRDFASMIQ
                     GSLFTQVRKADKNVRNLGVKQAPFMVLLGATMPSVLAEISFITNRQDAALLKTDKHRQ
                     RIAEALYGGVVRYQQSLKNVRVAAAQ"
BASE COUNT         2266 a         4290 c         4380 g         2356 t
ORIGIN      
        1 tcgtcgagcg cgagcatggc gcacggcagc gatcccaggc cgcccgcgtt gcacacagcc
       61 acggccaggg cgctcccctg gacgcccgcc atcggcgcct gaacgatcgg cagctcgatt
      121 ccgacaagct gctgcagtgt catccccgac gtcagcctgt cgctgattca gccgcttgtc
      181 aaccgagagg acgcgttgtc agcccagagc gtagatgatc ccgtcctgcg acgagatgac
      241 gatgcgtccc ccggcgatgg cgggggaggt tgtgatcccg gacccggcgt cgaactcgaa
      301 gagcttcttg cccgaggcga ggtccaggac gtagaggcgg ccgtcgccgg agccgacata
      361 aacgcggccg ccggcgatca cgggcgacga gtcgacgcgc gcccgcgtcg caaacgtcca
      421 gatcggattt cccgtcgcga tgtcgagcgc gtgaacgaac ttgtcacgcc cgccaagcac
      481 gatgcgtccg ctgccgagcg ctgccgagga gtagaagggg aactgccggt ccttgggtgc
      541 ataacgccag agacgccgcc gccggcccag gtcgaaggcc agcacctcgt tgttgaacgt
      601 gccgaagtag gcccggttgt tggtgatgag cggcgaggcg cccgtgtacg cccccgcttc
      661 gatctggtag acctctttac cgtcggccac cctgatcgcc cggagtaccg agtcgcagcc
      721 ggcgatgaac gccatgccgt cgtggatggc cggagtggcg tggaccatgc cgttggtctc
      781 gaacttccac ttgagctggc cggacttcgc atcgaggccg tacaggtgcg cgtcgtagga
      841 gccgatcagc acgacgtcgc cgacgatcac cggagaggac ttgatctccg agccggtcgt
      901 gaacgtccaa cgcttgctgc cgtcgtggac gttcaccgcg tgaagcagtc cggcaaggtc
      961 accgatgtac gccacgccgc cgccgacggc cggcgacgac tctccgatca gattcccggt
     1021 cgtgtacttc cagcgcagct tgccggtggc gaggtcgagc gcgaggagat ctccgtttcc
     1081 accgccgacg tacacgaccc cgtccacgat cgcggcggag gaatccacga gatcgcgcgc
     1141 atcgtaggtc cacacgacct tgagcgccgc cggaagcgta ccggaggaca cgccggtcag
     1201 gctcggcgtt cccctgaact gcttccattc gcttgccggc gccgccgcgg gcgcgggcgt
     1261 ttgcgcggta tgcgagggcg ccagaagaag cgcgaagacc aggatgaagc ccgcgcgcat
     1321 cgtcgttact tcgtggcgcc gatggcgaac agttggctgc ggttgttgag gaacagcgtg
     1381 ccgttggctg gcacgaccgt cgcgtacacg gcgctcccca tgttcatctc agcgatgacg
     1441 accttttcct tgctgtgcgc cagcacggtg acgtcgccgt cttcgtcgcc gagatacacc
     1501 ttgctgtcta tgacgaaggg tgagccccag acggcggcca gcatatcgtg aacccagtac
     1561 tcctggccgg tcttgacgtc gaggcagtgg aggtagccgc tgaagtcggc gatgtagagc
     1621 aggccgtcgg cgatcgcggc cgttgagatc gagcgccgga tcttgtcgaa gtgccacacc
     1681 agcccggtct tggtgatgtc gccccgcttg gtggcgtcga tggcgtacat gtgaccgacg
     1741 ccctcgccgt gctccggatc ctgcccgttg gcgatgtaga cgtgatcctg atagatgacc
     1801 ggcgtggcga tcagctcgtt gcgcgtcctc ggccacaccg agtcctttgg attggtgtcg
     1861 aactcccaga gcttcttccc ggtttcggcc tcgtagctcc ggacccagcc gtcaccctgc
     1921 gccgagacga cctgcagcac gccgccgatc cgccccaccg acggcgttga ccattggccg
     1981 tgcaggatgc ggttctcgac cgagttgtcc tcccaggcca gcttcccggt gttcttgtcg
     2041 agcgcgataa tcgacggcgc ccgcggcgac ggaatgttca cgtggctttc gtcctgaccg
     2101 ttcgacgtgc tcacgtagat caggttgccg aagacgaccg gcgaggagtt cgacaggttg
     2161 tgcgggtagg agccgacctc ttcgagcatg tcgaagctcc agatcacgtc cgcatccgcc
     2221 tgcccgacga acttctcgtc ggtgaccggg ccgtcgtttt ccttgtcccg gaagccctgg
     2281 atgtcgaggc acaacacgac gccccggttg ctcacgtagt acagccgctc gccctcgacg
     2341 gccggcgacg aggccacgcc ctgaaacggc cagtcgttgg cccgtccggc ttcgagtttc
     2401 gcgtgggtgt gctgccacag gaactcgccg gttgactccc ggaaggccat cagcacgccg
     2461 cggtcaccag gctgcttcgg gtctcggacg agctcgttgt tggtgcccac aaacaccatc
     2521 ccgcccgcga cgacagggtt gccgtagctc tgcgacccga gctcggcgac ccacttgatg
     2581 ttcttcttcg acttgacgtc ccactccgac gggacgttct tcatgttcga caccaggttg
     2641 cggtcgggcg tgcctcccca catcggccag tcgttggtgc cgggatcggc cgccgacagg
     2701 tacgcggtcg gcaaagcgca cgccagagcc gccagcaagg cgaggaagac gaggtggagt
     2761 cgcggtcgtt tgaaagtcgc catatctagt tgtaagtcct tgtgattcat cgtcctcgcg
     2821 gctactggtt tcgcgtgagc tcgaagttgt cgagatacgc gccgaacggg gcgttgatga
     2881 agagccctgg ggcgccctcc ctgtttccaa tcggatccgt cttgtcgatc atccaggcag
     2941 ccggctcggc ctggccgacg gcccacgctt tccccctggc gcgcacctgg ccgttcggca
     3001 ggttctcgac gcggaccttc aaccgatacc acgtgtctgg cttccattgg aacggcactg
     3061 tcacggtgcg cacggtttcc ggttcccacg gctcgagctt caactgctgg gccgtgccgt
     3121 agagcaccag cgaatagcgc tgggcggtga tgccgacgtc ggccatctgc cgcctgcgct
     3181 cgggcgcgcg cacgtcggcc tggaaggtgt aattcgacca gtcgggcggg ccgatgaacg
     3241 tacggccgcg ctggaagatc gtgttgagcg gctccttctg caggaccttc tgtccgtcga
     3301 gcgtcgccac gacgaggcgt cccggaacgg tgttggccca tcccggcggg atcgcgccgt
     3361 ccgcgtaacc gtcgaaggtc tcggtccacg gcagcgggtg agcgacgcgc gcgcgcgctt
     3421 cgccggtaag cccgcccgca gccgccttga tcacgccggc ctgtccaacg ggtttgttgg
     3481 cgaccgtcag cgcgccgtcg gcgatcgtgc cctcgagccc ctcgagcgac cacgtcgcct
     3541 tttcctcgcg caggaacctg ccggccgcgt cgaagagccg ggcgcgaagc ttcacggtct
     3601 gtcccggcac gaggttgagc tcggtgggcg acacctgcag gtgcgccggt gcgccggagc
     3661 cggccacggc cggttcatcg accgccaggc cggtgggcga agtcgcgctc ttcgcgccga
     3721 tggcgtagat ggcatcgctc gacatgaaga agatgcggcc gcgcgagatg gccgcgttgc
     3781 cgaggatctg ttccggggtc ccctcggatc cgcagcagct gttcgtgctg ttcggcagct
     3841 cgacctggct caggatctcg ccgcgatcgg cctgcggcct gacgatgaag aacgtgccgc
     3901 cgtcagtgcc gacgtagatt ttgccgtcgg cgagtactgg cgacgccttc tgcgcgagcc
     3961 ccatcggaag cgtccagatt tgcttgcccg tggcggtgtc gtacgccctg agcgtggacg
     4021 cgttgtcgag ctgatagagg cgcgtcccgt cgatgatcgg cgaggagtaa ccgaactcgg
     4081 tgccgcgaat cgcccatgtc gccgtcttga tgtcgccggt ctggctgccg tcgatggcgg
     4141 cgatgagtcc gagctcgacg ccttcgaggt tctcgtcccc gtgcgacagg aagacggtgt
     4201 tgccccggat ggcgacgccg gtattgatgg cgcgcttgga cacagggaag ccccacacct
     4261 tcttcccggt ctgagactga atggcgtaga cagcgccgtc tcccagtccg tcgatcagca
     4321 ggcgcatccc gttgatcgtc gcaatgctcg gcggggcata ggcggtgtcg tacgggcggc
     4381 cgcccgggtt ggcgacgtag atgacgtccc cggtgcgttt gtcgagagca atcaacctgt
     4441 gcgcccgcgc cgcggccgct ccccagttgg agacggcggc gctcacgatc acgaggtcgc
     4501 cgtcgacgac gggcgacatc gtccggccgc cgtgcgtcgt gaaggcggcg aactcctcgc
     4561 cgaacgatcg gctccacagg cgcttgccgt cggggctgag cgcgatcgcc tcggccccgc
     4621 cgctgaggac gtagatgttg cccgtctcgg gatcgacggc gggtgacgcc cacgcgatgc
     4681 gatgggcggg cacgtcgctc tggaagagat tgatgtggta ttcccagatg accttgccgg
     4741 tggcggcgtc gagcgccatg acccgttcct gcaggctggc gccgcgtccc acggggttct
     4801 gcacgtagac gcgatcgccc atcacgacgg gagtcgagcg cccgccggac gggacgcgcc
     4861 agaggaagtt ctccccgttc aacttccatg agctgatgag ccccgtctcc ctggagatgc
     4921 cgtcgcggtt gggtccgcga agctcgggcc aatctccgac ccgcacgcgg cgaggcccct
     4981 gggcgggcag caccacgctc aacgccatca cgacggtggc caccaaccaa catgtcttcc
     5041 gcatcaaggc accctcagcc cgtccgttac gccagcagac gtaatcgagt caatacgctg
     5101 ccaacccgag cgctgtttac gaatggccgc gttcccccgc gaccaggcgc tccctagatc
     5161 ctagcatcgg cctgcatgcc gtcgccccac gattcgtccc tctcgttgcg cggactggga
     5221 ggccgtgcat aatggacggt catgactcat cgccgcgttg catcgtaccc tcagcagttg
     5281 gccgcgcgtg cgaccgcgtt gtcggtcgcg tcggctctcc tgcttactcc gtcagccgct
     5341 cgaggacaat cgacccagtc gcagaccacg caacctgcag cgacgtttca gctgccgacg
     5401 ctcatcgtga cggcacagaa agagccggcc gacgcccaga cgcttccgct cagcctgacc
     5461 gccgttccga agacgacgtt ggagaacgca ggcgtgaaca tcgtgagcga ggcgggtgcg
     5521 tacggtccca atctgcactt cacggagttc accgcgagga agctcagtaa cgcgcgcttc
     5581 cgcggcattg gcggcagtcc ggccaacccg tcggtcacca cactctacga cggcgtgccc
     5641 caactgcacg ccaacacggc aagcctcgac tttctcgacg tcgagcaggc cgagttcgtg
     5701 cgcggcccgc agggcacgct cttcggccgg aacacgctgg gcggtctcgt caacatcacg
     5761 agcattcgtc cgtctctcgc cgactggtcc ggcaccttct cggttccctt cgccaacaac
     5821 gcgtcccggg atgtgcgagg cagcatcaac ggtcccctcg cggacgggaa gatgggcgtc
     5881 gccctctcga tgcactacgg ccgccgtgac ggctacacgg tcaacgatct cacggggaac
     5941 gatctcgact ctcgttccgg cttcgccgcg aagggccagt tcctctggac gccgacgacc
     6001 gcctgggaga cgcgcgtcat cgtcaacggc gagcgcgatc gggacggcga ctacgcgctg
     6061 agcgacctcg agggcctgcg gcagaacccg tttcacaccg cccgcgactt cgagggccac
     6121 accaaccggg acgtgctcgc gacgacgatc gtgaaccggc acgagggaca gcgggttgcc
     6181 gtgtcgtcca cgaccggctt cgtgaagtgg gacaccgagg acgcaaccga cctggactac
     6241 tcgcctctgc cgctcctgac gcgcaacaac gtcgaggaga gctttcagtt cacgcaggaa
     6301 gtacgtctgt cttcagcagc caaggcgccg gttcgactgg gcggcgactc gaggctggcg
     6361 tggcaggccg gagtgttcct gttcacgcag aactacgacc agaacgccgt caacacgctc
     6421 gggccgatct tcacctcttt gccgttcgac gtcgccaaca cggcccccct ggctgcgctc
     6481 gacgatttcg gcgtcggcgt gtacggacag gggacggcca ctctggcgac gcggctcgat
     6541 ctgacggccg gtgttcgcgt ggactacgag cagaaggacg cgaatctcag cacgttctac
     6601 acgcccgctc tgccgttcct tccgccgcag cgggacatcg ttgcggacga gagctacgca
     6661 aacgtctcgc cccagtttgc cgctgcgttt cacgtccagc cggggaagac gatctacgcc
     6721 gccgtggcta acggatatcg cgcggggggc ttcaaccctt cgtctccggt tggcagcgag
     6781 gcgtacggcg aagagcacac gtggagcgtc gaggggggag tgaagacggc gtgggcaggc
     6841 gaacgcgtga cggccaacgc atcggtgttc tggatcgact ggagcgacct gcagacgaac
     6901 gtgccgaacc cccaggtccc gggagagttc tacatcgcca acatcggagg cgcggcgagc
     6961 tccggcgtcg aggtcgaggt caacgcccgg gtgcatcccg gcgtagatgt gttcgggtcg
     7021 gtgggctaca cgcatgcgcg gttcgacgag gggagcacgt cgagcggcgc ccccgtcggc
     7081 ggcaacatca tcccgaatac tcccgactat acgggaacga tcggcgttca gatctcccac
     7141 gacgtgcgtc agggcgtgtc gctgtatggt cgggccgaag ggatcttctc gggcgcgttc
     7201 atgtacgacg acttcaatct cgaagggcag gacgcgtact cgctcgccaa cttccgggcc
     7261 ggcgtccgga gccggtgggt gttcgttgaa gcgtggatcc ggaacgcgtt cgacacccgg
     7321 tacattccga cggcgttcgc gtacggtccg ctctcgccat ccggcttcat cggcgagatg
     7381 ggcaacccga ggacgttcgg cgtcagcgcg ggggtgggat tctgagaagt acctcgattg
     7441 tcgcgacggc cggcgccttg attctcgggt gcatggcctt gatggcgcag acgccatcga
     7501 ccgactggcc gcagtggcgc ggaccggacc ggaatggcgt gtcgaaggag accgggcttc
     7561 tcaagcagtg gccgaagtcg ggccctgcgc tcgtgtggtc cgcgtctcag ctcggtggcg
     7621 ggtacggctc ggtggccgtc cgcggcgatc gcgtgtacct ccagggcatg aagaaccgtc
     7681 agagcatcgt gacgagcctg gatcgcgcga cggggaagtt cgtctggtcg gcccagctcg
     7741 gatcatcagg gaataacgac cggggatccg gcccgcgcag cacgccgacg gttgacggcg
     7801 accgcgtgtt cgtcctcacc gagaacggcg atctcgcgtc gctgcaggcg gccgacggga
     7861 agatcgtgtg gcgccggaac atccttcagg agttccgcgg gcgcaacatc aactggctga
     7921 tcagcgagtc gccgctcgtc gatcgcgacc tcgtcatcgt cagccccggc gggcggaatg
     7981 ccggtatggt ggcgctggag aaggcgaccg gcaagaccgt gtggatcagt aaggagttga
     8041 gcgaggaggc ggggtacgcg tcgccggtcg tggccgacgt gcagggcgtc cggacgatca
     8101 tgacgttgac gagcgaagcc gccgtcggcg tccgtgcctc ggacggcaag ctgatgtggc
     8161 gctacggcaa cgtggccaac gggacggcca atatcacgac gcccgtatac gcggacggga
     8221 aggtgttctt cacgtcggcc tacggcaccg gcggcgccct tctcgggctt cgagcggccg
     8281 gcgaagaaaa tcgcgcccag gagatttact tcacctcgaa catgcagaac catcacggcg
     8341 gcgtggtcct cgtcgatggt tacttgtacg gcttcagcaa ctccatcctg acgtgcatgg
     8401 agttcgcgac gggcaaggtc atgtggcggg acaggagcgt agggaagggg tcggtcgcgt
     8461 acgccgacgg ccatctctac atcctgagcg agaacaacgt cgtcgggctc gtcgaagcgt
     8521 cgccaaccgg ataccgcgag aaagggcgct tcccggttcc cgatcaaggc tggtcaagct
     8581 gggcgcaccc ggtcgtcagc ggtggccggc tctatatccg gaaccaggag atcctcgcga
     8641 gctatgatgt gcgggcgagg tagacgagag tgtagggggc agggggcagg gggcaggggg
     8701 cagcggcaag ggggaagtca gagtcacgat ttcattccgc aggaggctca tcgcatgtcg
     8761 tcttcatctc gtatctcggt cgcgctcgtc gtgctcgcag ccctcgtgat gcctgtgtcg
     8821 gcgcagcgcg cctcgaatga ctggactcag tggcgcggcc ccagccgcga tggcgccgcc
     8881 ccggccgttg ccgacgtgag cgcgtggccg gaaaaattga cgcagaagtg gaaggtcgag
     8941 gtcgggcagg gatacgcgac gccgcttgtg gtcggcaacc ggatctacca gttcgcgcgc
     9001 aagggcgagc gcgaggtcat gatggcgatc gacgccgaca gtggcaaggt gctctgggag
     9061 acggggcaca ccgcgacctt caccatgatg cctgccacga agcagcacgg ccccggcccg
     9121 aaatcgacgc cgctattcgc caacggccgc ctctattcca ttgggatgac gggcatcgtg
     9181 acggcctacg acgccgcctc gggcaagcag ctctggcaga agcccggttc gaccgtcgtg
     9241 ccgatgttca cgacacattc gttctcgcct gtcatcgacc gcgggctcgt cgtgtttcac
     9301 ctcggcgggc acgacgaagg cgccttgacc gcgttcgacg cgagcacggg cgaccagcgg
     9361 tggagctgga agggcgacgg tccgggatac ggctccccgg tcctggccga cttcggaggg
     9421 acccggcaga tcgtgaccat cacacagggc aagctcgtgg gcgttgacgt ggctacgggc
     9481 gcgctgctct gggagcggcc gttcgtcagc ccgaacaaca cgaactcgaa cacgccggtc
     9541 gtcgcgggtg atctgctgat cgtgtcgagc aacggcccgc cgacgtccgc gcttcgggtc
     9601 agcaagcagg gcgacaagtg gtcggtggaa accgtgtggg agaacgccga cgtgcccatg
     9661 cgcctgagcg acgcagttct cgccggcgac gtgctgtttg gcctctcgac gaggaactcc
     9721 ggccagtact tcggggtcga tgtgaaaacg gggaagacgc tctggacgtc ggaagggcgc
     9781 cagggcgggc acgccgcgat tgccagggcc ggaaacctgc tcttcagtct cgaagacgac
     9841 ggcgagttgg tgatcgtgca gaacagccgg acagcgttcg agccgctgcg caggtacaag
     9901 gtcgccgacg tcgcgacgtg ggggcagccg tcctattcag gcaaccgtgt gttcgtgaag
     9961 gacgagacga acctgacgct ctggacgacc aagtagctgg tagccggtag cttcgcacct
    10021 actgccttgg ggcggtggcc caccacgcgt gtcacgaacc acggagacac ggagacgcgg
    10081 aggtgggttc tcggtgacgc cgccttcggc accgcggcct cgcaagacgc cgggcggtcg
    10141 actggtctgc gccatgcact tccgttaggt ggaagtgtct actttatcga ggcaagatct
    10201 gacctccggt tgtctcgcgg gttagcctgg cggccgcacc gctgcatggt tcgcagcgct
    10261 gtgttcccgg aggtcagcca tggccaagtc tactccgctg ttcgtcgggt tggacgtgca
    10321 caaggattcg gtcgcggtcg cgctatgcga cgggtgggcg gcctgatccg ccggtgttcg
    10381 tggaacgttg cgaatgagcc gcgcgccgtc gcagttcagc ggcgcgtcga gacagacgcc
    10441 tcggccaggc ctgagaagac gttgacctgc gcttccatcg aggtgacgct gcgccgcata
    10501 ctcacccaac catcgctggg cccgcgacta gcgcctcacc aagttgcgcc aagtcaggcg
    10561 ttagttcccg gccgaggtag cttatgtagg cctctggctt gccatacacc cgcggcttga
    10621 ccagtgcgag cgcccggaag ccgaacgaat tagcgatacc tagctgcacg tctccgccga
    10681 tgctcggtaa cgactcatcg gaaatgcaat cttggatgac gtacctaggc attcgactca
    10741 gcggccggcc cggcgccgcg ctgccaagag cggccgcgaa ccgctccatc attcgggact
    10801 tgtcggcgcc caagtaaaga ggatgcccca cctgaatatc ttcccgttca gagcatttca
    10861 gagtccaggt accggcttca tcacggcccg gttcaacatg aaacgccgtg agccgattcg
    10921 tgcggccgca atggccgaag atggcgacct cgaacatggc gctctctccg actttggatt
    10981 tgaagtcgtc gaacgtgtgg agtaggtagc ggtgaacgta gctggccaca tcggccatcg
    11041 aaggcacgta gtccgctcga gcagccaaat tgccgagcag tggcacgagg gccaaatacg
    11101 cgttctggcc catcagcgta ctgccggcga agcaatatcc gaatgagtgg gtgtagctaa
    11161 tcctagagaa gaagccatct tgaccaggcg ctcgacacac taccggcaac gagaagatct
    11221 ttgcgccgtc ctctatcaac ggcgccgcag acgctccgct gattctgctg tcggcgacga
    11281 tccacagcgt cggatgcttc ggctcttcgt cgttgagcca gatggctatc gctgtcattc
    11341 ttgtatccct aactactagt gaccgccggg ctgcgcgtaa ccaactggta ctgctggcgc
    11401 ggtccacgca gtaacccatc gaatcgaacg ggattctagc agacactctt ctttgggtga
    11461 cgcgtggtta ggcgcgaatg tctggcggaa acgttctccg tgtctccgcg tctccgtggt
    11521 tcgtggaggg ggacgttgga ctactgcgcc gcggccaccc gaacgttctt gagcgaccgc
    11581 tggtagcgga cgacgtcgct gtagagcgcc tcggcaatcc gctggcggtg tttgtcggtc
    11641 ttgagatagc cggttgctac cgtgcggcgg gaatgccgta accgccaagg ggcgtcgttt
    11701 ccgtgcggtt gctgaaacca gcaatgagcg gacggccctt tgcaatggcc gcctgcactg
    11761 ccggtagctt cagcgaagcc tggtgcttcg cctggtcttc ccacacctcg gtcacccaga
    11821 gcgcatctcg gtccgccgtg tcggccgcga ccacgtagct gagacagcca ggcatgccgc
    11881 cagtgccatc aatcagaata ctggcgaggg cctcacgctg gccagccacg gccgtgatct
    11941 tgccaataag accgaacatc gtgcctccag taatctgggc cgctgttacc cgcacaccat
    12001 cggcggcaat cagcgcgccg gccgccacca ggaactggcg ccgcgcgggc cccgctgccg
    12061 tcaggtgcag cggccggtta gcctcacgcc gccgaacgtc gatagaccca cctccaagtc
    12121 cgatgacctc gtcacccctc ggtgagacaa tcgaccagcc ttcgtaactg ctcgacgttg
    12181 tgagaacctc gagtctcgag ccgttggcga agtccaccac cacatcacca gtttcccgag
    12241 cgatcgacgc gtgccgtact gttgcttcag caagagagcc tttcgctagg gcggctgcat
    12301 caacgggctg ctgtagcccg aattgatgcc cgtgatcgac gctagtcgcc tgtacctttc
    12361 ccttgctaat gatccgccac agcgtctctg tgcgaagcgt gccgccacct acgaattcga
    12421 aatgccaaga gcttggttcg acgaacgata tcgctcggac tctggcgcca atcatccagc
    12481 tgagatcaga agaaggcata ccccgtgatg aatagctgct agtagatcgc cgggccgcgc
    12541 gtaatcagca ggtgctgccg gagcggtcca cgtcgtaccc gctcgaatcg gacgggattc
    12601 tagcgtgctt ttcagatcgg tgtagctcgc agtttgggtc cgcagaggac caaggacgag
    12661 ggaccaagga cggaccaagc accgcggacc atgaactaag gacgacgcta caggaaaagg
    12721 aaaagagtcc ctactgcgcc gcggccaccc ggacgttctt gagcgactgc tggtagcgga
    12781 cgacgccgcc gtagagcgcc tcggcaatcc gctggcggtg cttgtcggtc ttgagcagcg
    12841 cggcatcctg cctgttcgtg atgaacgaga tctccgccag gacgctgggc atggttgcgc
    12901 cgagcagcac catgaacggc gcctgcttga cgccgaggtt tcggacgttc ttgtcggcct
    12961 tccgcacctg cgtgaagagt gagccctgga tcatcgacgc gaagtcgcgc gactcgtcga
    13021 tcttgttgtt gagcgcgatc gccttgacga tgtcgggcag ctggcccatg cctcgccccg
    13081 acgacgcgtt ctcgcgcgcc gcgactgcct cggcggccgg gttggtggtc aggttgagga
    13141 agtaggtttc gatgcctcgc gcggccgtgt cggtgctggc gttcgcgtga atcgacagga
    13201 agaggtcggc gccctcgcgg ttggcgatcg ccgtccgctc ttcgagcggg atgaacgagt
    13261 cgtcgcgcct cgtcatcacg accgcgaatc ct
//
LOCUS       DBNGSH010000575         3109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2265009, whole genome shotgun sequence.
ACCESSION   DBNGSH010000575 DBNGSH010000000
VERSION     DBNGSH010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2265009"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="AC16CY_13285"
     CDS             complement(<1..731)
                     /locus_tag="AC16CY_13285"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3354814.1"
                     /translation="MKSVRLVTISLAAALAGLPLARPSAQAGASASMWQALVRVPLPA
                     DSEAVISVNGLMMPADPVAEHSHAGPTIGYIVAGEIENQVAPDPPATLRPGGHFYEAP
                     RQLHKMMRNLSAEPAKLLVFHAGRTGVPASLLKALPAEPTTLSFATPTQWQVPLPSTA
                     NQELRLIRLTLPVGSRTEVAPHTGPGMVYVLEGSMSVVGGGSPQPQTYSAGDLFLDPA
                     NREGLIYRNLTGKEPAKLLLYHVSDP"
     gene            complement(728..1138)
                     /locus_tag="AC16CY_13290"
     CDS             complement(728..1138)
                     /locus_tag="AC16CY_13290"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3354815.1"
                     /translation="MESRRTFLELAAAALLPVGAPSQAAAQTVEGDQPLGVIARHALT
                     EALDGYEIVLTRSRMRYFPGSSTPVHRHPGVVLAYVLEGRVRFAINNEPEQIVSAGST
                     FFEPPGAVHTTSGSASAAENTRILVFEVRPKEQV"
     gene            complement(1156..>3109)
                     /locus_tag="AC16CY_13295"
     CDS             complement(1156..>3109)
                     /locus_tag="AC16CY_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3354816.1"
                     /translation="GSQVWLSGVPYTLIGVMDPAFRFVRHAALGPPQEPDLYLPFAFH
                     LADQDPRDPNHTTFAALMRVRAGTHSDRVAAAVDAVERVVNARNRQTPGVRLQAIRLQ
                     DDLIAPIRPVLVTLGLAGVFLLLVLTVNLASLLLARAGAREREFAVSRALGANGAAVL
                     RAMVIEGGALGLMGGVAGALTGWWGGRVLVSLAPLDLPRRSEIALDWRIAGVIVAAGV
                     GVGVIAAALPAVWISRALLAVSLVTASGRGAGAPQRWRRGIVVAQVALTVVLLCAGGL
                     VVHSFLRLLATDPGFSPANVLTFRVTMDPRLFPAAADAFAFQDRVESALTALPGVTSV
                     GATAALPLTTSGPQNSVWSWQEKVRFPGAPGNTGDAQRDTVLVDVIAARGGYPGAMGM
                     RFVDGAPFARSRSQGVREALIDRQLAERFFPTGTPIGASIPFVQGKALRVVGVVTQTR
                     LASLHEDGRPQLFIRAEDWVRYMPVWVIKTTGDPRALAADVRQMIHRIDPRIPVSAVQ
                     TMDEIVRDALRQQRISTVMIAGFALGALLLAAMGLFGTISGSVVRRRGEIAIRLALGA
                     THQRMVRLALGEGALLVMAGMVIAVPGVYAAGDVIRSLLVDVSPWDPLTLSAVALGLL
                     VVTLTACYVPARRVLRIDPASLLRQE"
BASE COUNT          543 a         1088 c         1051 g          427 t
ORIGIN      
        1 ggatcgctca cgtggtacag gagcagcttc gcaggctctt tcccggtgag gtttcgatag
       61 atgaggccct ctcggttggc tgggtccagg aacagatcgc cggcactgta ggtctgtggt
      121 tgcggcgagc cgccgcccac gaccgacatc gatccttcca gcacgtagac catacccggg
      181 cccgtgtgtg gggcgacttc cgtacgactg ccaacgggaa gcgtgaggcg aatcaatcgc
      241 agttcctggt tggccgtcga cggcagcggc acctgccact gagtcggcgt ggcgaacgac
      301 agtgtcgtgg gttcggccgg cagcgccttc aggagtgacg cgggcacgcc ggtgcggccc
      361 gcgtgaaaga cgagcagctt cgcgggttcg gcgctcaagt tgcgcatcat cttgtgcagt
      421 tgcctgggcg cctcatagaa gtgcccgccg ggcctgagcg tggctggcgg atccggcgcg
      481 acctggttct cgatctcacc ggccacgatg tagccgatgg tcggaccggc gtgcgaatgc
      541 tcggcaacgg gatcggcggg catcatcaga ccattgacgc tgatgaccgc ttcgctgtcc
      601 gcgggcagcg gcactcgcac gagggcttgc cacatcgaag cgctcgcgcc cgcttgcgcc
      661 gacggccgtg ccaatggcag accggccagt gcggcggcca ggctgatggt gaccaatcgt
      721 acggacttca tacctgctcc ttcggccgca cttcgaacac caggatgcgc gtgttctcgg
      781 cggcgctcgc ggaaccagac gtcgtgtgca cggcgccggg cggctcgaag aacgtgctgc
      841 cggcagagac gatctgttcc ggctcgttgt tgatcgcaaa ccgcacccgc ccctcgagca
      901 cgtacgcgag cacgacgccc ggatgacggt gtacaggggt ggacgacccc gggaagtagc
      961 gcatccggct cctggtgagc acgatctcgt agccgtcgag cgcctcggtc aatgcgtgcc
     1021 gagcgatgac gccaagcggt tggtcaccct cgactgtctg cgcggcggcc tgagaaggcg
     1081 ctcccacggg cagcagtgcc gccgccgcga gttcgagaaa cgttcgacga ctctccatga
     1141 tgacggtctc tcctcttatt cctggcgcaa cagcgaggcg ggatcgatcc tcagtacgcg
     1201 gcgcgccggc acgtagcacg ccgtcagcgt gacgaccagc aggcccagcg ccactgccga
     1261 caacgtcagc ggatcccacg gcgagacgtc caccagcagg ctgcgaatga cgtcaccagc
     1321 ggcatagacg cccggaaccg cgatgaccat gccggccatc acgagcagcg cgccctcgcc
     1381 cagcgcgaga cggaccatgc gctggtgcgt tgcgccaaga gccagacgga tggcgatctc
     1441 gccgcgccgg cggaccacgg agccgctgat cgtgccgaag agtcccatcg ccgcgagcag
     1501 cagcgccccg agcgcgaaac cggcgatcat caccgtgctg attcgctgct ggcgcagcgc
     1561 gtccctgacg atctcgtcca tcgtctgcac cgcagaaaca ggaatgcgcg gatcgatccg
     1621 gtgaatcatc tggcgcacat ccgcggcgag tgcccgcgga tcgccggtgg tcttgatcac
     1681 ccacaccggc atgtagcgaa cccaatcttc ggcacggatg aacagttgcg gccggccgtc
     1741 ctcgtgcagg ctggccagac gcgtctgcgt caccaccccg accacgcgca gcgcctttcc
     1801 ctggacgaag gggattgacg cgccgatcgg agtcccggtc ggaaagaagc gctccgcgag
     1861 ttgccgatcg atcagcgctt cacggacgcc ctgggagcgg ctgcgcgcga acggagcacc
     1921 atcgacgaag cgcatgccca tcgcgccggg atagccgccg cgggcggcga tgacgtccac
     1981 gagcacggtg tcgcgctgcg cgtcgccggt gttgccgggc gcacctggaa agcggacctt
     2041 ctcctgccag gaccacacgc tgttctgcgg cccggacgtc gtcagcggca acgcggcggt
     2101 ggcgccgacg ctcgtcacgc cgggcagcgc tgtcaacgcg gattcgacgc gatcctggaa
     2161 cgcgaaggca tccgccgccg ctgggaagag ccggggatcc atcgtcacgc ggaacgtcag
     2221 cacgttggct ggcgagaacc cgggatccgt ggcgagcagc cgcaggaagc tgtgcacgac
     2281 gagcccgccg gcgcagagca gcaccaccgt cagggcgacc tgcgccacca cgatcccgcg
     2341 ccgccaccgc tgcggcgcac cggcgccgcg gccgctggcc gtcacgagtg aaacggccag
     2401 caaggcgcgc gatatccaca cggccggcag tgccgcggca atgacgccaa ctccgacacc
     2461 cgcggcgacg atcacgccgg cgatgcgcca gtcgagcgcg atctcgctgc gacgaggcag
     2521 atccagcggc gcgagtgaga cgagcacacg gcccccccac caacccgtga gcgcgccggc
     2581 cacgcccccc atcaggccca acgccccgcc ttcaatcacc atcgcccgca gcacggccgc
     2641 gccattggca cccagcgccc gcgacaccgc gaactcccgc tcgcgtgcgc ccgcccgcgc
     2701 cagcagcagc gaggcgaggt tcacggtgag cacgaggagc aggaacacgc ccgcaagacc
     2761 aagcgtgacc aacacgggtc ggattggcgc gatcaggtcg tcctgcaggc ggatcgcctg
     2821 gagccgcacc cccggggtct gccggttgcg cgcgttgacg acgcgctcga cggcatcgac
     2881 cgccgccgcc acgcggtccg agtgggtgcc ggcgcgcaca cgcatcaggg cggcaaacgt
     2941 ggtgtggttc ggatcgcgcg ggtcctgatc ggcgagatga aacgcaaacg gcaggtacag
     3001 atccggttcc tgcgggggac cgagcgcggc gtgccgcacg aatcggaagg ccgggtccat
     3061 gacgccgatc aaggtgtagg gcacgccgct cagccacacc tgcgacccg
//
LOCUS       DBNGSH010000576         8969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3962452, whole genome shotgun sequence.
ACCESSION   DBNGSH010000576 DBNGSH010000000
VERSION     DBNGSH010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3962452"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="AC16CY_13300"
     CDS             <1..1383
                     /locus_tag="AC16CY_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354817.1"
                     /translation="RRSALVAATVFGFYPYLWKAAGWDQHDGVGNVYYLLTTAFLTLA
                     AASRVPSRPYLCCAGVTCACAVYCNLTWAFLLPSFGPYWLAARKARGLPVTLGLTLLW
                     SGAGFLLLTLFFAIVNYGLGGSLNFYLPSITYALEAVKTPSTYTAPDAAWMTSSPWLF
                     LPAAAVLAGPVALVRARQRQSSYHARMAVALYVNLLFCAGVLILWELAGEPMLQTSYY
                     GSYLLAPTFLFLGVTTFSVPETLRPSRFFALLAGVVGVSALVWWDSTGGTWLWLLGTG
                     WGPVLAIAVVVAAGLVAGRTRAVAMTVLGVSILSLNSRLMPAGVGWGRPSQAEDAFLR
                     IVEGIEIAMDAARAQPDRFIRFWYEASDVRSGEFSSINSAYFYLYSKFPDYPMPPDWR
                     VEGLTAVLSSLPDEKRGEMFATAQSLLRQRGFRARVAADHVIDRGGVRYVMTIIEIEQ
                     DAQADRDPAA"
     gene            complement(1356..2525)
                     /gene="rffA"
                     /locus_tag="AC16CY_13305"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(1356..2525)
                     /gene="rffA"
                     /locus_tag="AC16CY_13305"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose:2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HIR3354818.1"
                     /translation="MTSIPFNRVHLLGSEIDYMRAALDNMQICGDGAFTKRCQTLLEE
                     SLGVPKALLTTSCTHALEMSALLLDLQPGDEVIVPSFTFVSTANAFVLRGARPVFADI
                     RPDTLNLDESRLEPLITKRSKVIVPVHYAGVGCEMDAIVEIAARHGLAVVEDNAHGLF
                     GRYKGKYLGTFGCLATQSFHETKNFTCGEGGALLINDRALVERAEILREKGTDRSRFF
                     RGLVDKYSWVDVGSSYLPSDLLAAFLCAQLEQRERVQEDRRRAWNLYSTSLKVWAAEH
                     NVRLPVVPPHCEQTYHMFYMLLPSLEDRQALITHLRNRSIHGVFHYTPLHTSTMGRKF
                     GGKEGACPVTEDVSDRLLRLPLYNRFPEQQQMEVIEAVSSFAEWKRGQAAGSRSA"
     gene            complement(2522..3400)
                     /locus_tag="AC16CY_13310"
     CDS             complement(2522..3400)
                     /locus_tag="AC16CY_13310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3354819.1"
                     /translation="MKRCLACEGTFDRDDWRCGRCGWRPAESDGFVAFAPDLAQSNDG
                     FSADYFDQLRAVEARHFWFRARNRLLSWTFDSYFPGARSFLEIGCGTGFVLAGMRDRF
                     PDVRLTGSEIFTGGLRIAQQRVPGVTLLQMDARKLPFDAEFDVIGAFDVLEHVDDDQR
                     VLGEMFRSCRPGGGVMLTVPQHQFLWSRMDDYAHHKRRYGRRDLVDKVERAGFQTIRV
                     TSFVSILLPLMLISRWQRNRATADFDPLSEFRIGPLLNGTLEGILAAERLLITAGVSF
                     PAGGSLLLAARKKEAP"
     gene            3474..4019
                     /locus_tag="AC16CY_13315"
     CDS             3474..4019
                     /locus_tag="AC16CY_13315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3354820.1"
                     /translation="MGKLTLRADVAIGSLEKSHAADMLAWMQDSEISANIGLTRSPTM
                     EATEAWIASALSGTSTWPYAIFHAGAHVGNVVLDQRDTHLGSARLSVYVGPATSRGRG
                     VGVTGMYLALRDAFASRGLHKVWLTVHARNSRAISAYTRLGFEVEGVLRDGFLLAGER
                     VPALYMGLLRGDFLALQTETV"
     gene            4016..4705
                     /locus_tag="AC16CY_13320"
     CDS             4016..4705
                     /locus_tag="AC16CY_13320"
                     /inference="COORDINATES: protein motif:HMM:NF020463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HIR3354821.1"
                     /translation="MTCVILQPSYIPWRGYFHQIQKADYFVFYDDVQYDAHGWRNRNR
                     IKTPQGPQWLTIPVRTKGHLTEQRAICQMEIDWDRPWNRKHLASFRQAYRHAPFYNEW
                     DPFLAEVYGRTPSHLAPFTIDVTIELAHRLGIAHTHFLRSSELRVPGQRTDRILAIVR
                     HLGCDRYVSGPSARRYLEEDRFADAGVALEYMQYDYPEYPQLHSPFDADVTILDLLLM
                     TGPDAPKYIWG"
     gene            4717..5682
                     /locus_tag="AC16CY_13325"
     CDS             4717..5682
                     /locus_tag="AC16CY_13325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3354822.1"
                     /translation="MPKLSIVVPIYQDGYLVKPFCEELQKQMRLMLRADDISRDVEVI
                     FVNDGSPDDSQALLRDAARAFPFVKVIQLSRNFGQHVAVTCGYRFASGDYVAMMNVDM
                     QDPPDQIPRLLERLEQGDCDIVIGLRQHRRESWHEDLTSHAFNLLLNTLTGSRSPLNT
                     ASLRMMNRRFVDAYNTLSERTPFIPGLEDWLGFRRDYVPVRNQARAMGRSSYTFHKRL
                     SMAVESIVGFSDLPLRIAAWTGFVITGIGAVLNVYLLSHWLFFRDTLPGFTSTFSIIV
                     LLGGANLMLLGLVGRYVGRILREVQGRPRYVIQSFENLLPRRDEP"
     gene            complement(5659..6519)
                     /locus_tag="AC16CY_13330"
     CDS             complement(5659..6519)
                     /locus_tag="AC16CY_13330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR3354823.1"
                     /translation="MDATAEPRYQQVLEANRKVHTALAGVYNTTEPHFRPENVAYVER
                     TLRKIASETGARKLLDLGCGTGFVIGIAKKFVAEIDGVDITEAMIQQVDRSGGADIRL
                     HRCDTGTFPVEAGAYDMVTAYSFLHHLYDIEPTLATAARALRRGGKFYADLEPNRDFW
                     NAVKSLDPGVVYDPIVERERAAVLEKDTEIQDKYDIDREVFNNAEYNKNVTGGFAEEY
                     LRERLIAAGFASIEFFYYWFLGQAALVNDERYEREQRLLAADLCADTLTRGLPLTRHL
                     FKYLGFVATR"
     gene            complement(6521..7306)
                     /locus_tag="AC16CY_13335"
     CDS             complement(6521..7306)
                     /locus_tag="AC16CY_13335"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR3354824.1"
                     /translation="MAASTTKEAFTRAIESQGYATLPGVIPEALLAPLRAELEAAIAT
                     ERELTERRYPGAHDGMVLLCSLYGGLLVDVLGEPSLMAPFEWILGEGCIIYAYTSSSM
                     PPDGTNYSTRIHVDCPRMIPGYITNVGATILLDDFTEQNGATWFLPGSHTRALEPTEK
                     EFYAKAQRVLAPAGSVFYFDARVWHAGGRNETDRWRHALTINMCRPYMKQRIDIPRAM
                     AHMDLSGTSERVLQKLGFLAQVPANYDEYYAPPAERKYRQKVE"
     gene            complement(7318..8238)
                     /locus_tag="AC16CY_13340"
     CDS             complement(7318..8238)
                     /locus_tag="AC16CY_13340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3354825.1"
                     /translation="MIPVYNSEAGLPALLDRLRPVLQGLNTTFEIILINDGSRDRSWA
                     AIESLSRRFPEIAGIDLMRNYGQHNALLCGIRASRHDIVVTLDDDLQQPPEEIPRLLS
                     KLAEGYDVVYGRPAQQQYGLWRNVGTRIAKAALGTATGGAVAPMVSPFRAFRASVKPA
                     FQEYRSPLLSIDVLLTWATTSFASVAVRSDPRRSGPSNYRFRSLVRHAMNLMTGFSLI
                     PLQLATIVGFAFTLFGLAVLAYVLGRYLVAGTVVAGFAFLASIIAIFSGAQLFALGII
                     GEYLGRMHLRTMERPAYIVRSATPPRGAST"
     gene            complement(8265..>8969)
                     /locus_tag="AC16CY_13345"
     CDS             complement(8265..>8969)
                     /locus_tag="AC16CY_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354826.1"
                     /translation="LVQTLVRGSPDSRLPDVANPIAVVAAWVSARCLRAAVGAKPWVR
                     RATMTLVAAGIVITAWSVGTFAETMANVRASRILSEGIGRRVIATAELLQARPIDAWP
                     REASGLGGLMRYVFECTADTDRLFVTWFGPDVYFTTERRFAGGQAYLVAGWHASPEDQ
                     RLTVSRLQQQRVPIVIEKTSVDYEAAFPLVAEYVRSRYGEVPVKSDSMRDYRVLVDKG
                     LTPTGTYEPLGTPCYR"
BASE COUNT         1479 a         2964 c         2885 g         1641 t
ORIGIN      
        1 cgccgctcgg cgctcgtggc cgccaccgtg ttcggtttct atccgtacct gtggaaggcg
       61 gcaggctggg atcaacacga cggcgtcgga aacgtctact acctgctgac gacggcgttc
      121 ctcacgctgg cagcggcgag cagggttccg tcgcggccct acttgtgttg cgcgggggtt
      181 acgtgcgcct gcgccgtgta ctgcaatctc acctgggcgt tcctgctgcc ttccttcggg
      241 ccgtattggc tggcagcgcg gaaggctcgg ggcctgccgg tgacgctcgg gctgacgctg
      301 ctctggtctg gcgccggatt cctcctgctc acgctgttct tcgccatcgt gaattacggc
      361 ctgggcggct ccctcaactt ctacctcccg tcgatcacgt acgccctgga agccgtcaag
      421 acgcccagca cgtacacggc gcccgatgcg gcgtggatga cctcatcgcc gtggctgttt
      481 ctgcccgcgg cggcggttct ggcgggcccg gtcgcgctgg tgcgtgctcg acagcggcaa
      541 agcagctacc acgcgcgaat ggccgtcgct ctctacgtaa acctgctgtt ttgcgccggc
      601 gtgctgatcc tgtgggagct ggccggtgaa ccaatgctgc agacgtcgta ctacggcagt
      661 tacctgctgg cgccgacatt cctctttctg ggtgtcacca cgttctccgt gcccgagacg
      721 ctccggccgt cccgattctt cgcgctgctc gccggggtcg tgggcgtgag cgcgctggtg
      781 tggtgggact ccacgggagg gacgtggctg tggctgcttg gcacaggttg gggtcccgtg
      841 ctggccattg ccgtcgtggt cgcggcagga ctcgttgccg gccggacccg agccgtggcg
      901 atgacggtgc tgggcgtcag catcttgtca ctgaattccc ggctcatgcc ggccggtgtg
      961 ggctgggggc ggccgtctca agcggaggac gccttcctgc gcatcgtcga gggcatcgag
     1021 atcgccatgg acgccgcccg cgcccagccc gacaggttca ttcgcttctg gtacgaggcc
     1081 agcgacgtgc gctcgggtga gttcagctcg atcaacagcg cgtacttcta cctttactcc
     1141 aagtttcccg actacccgat gccgcccgac tggcgcgtgg aagggctgac ggcggtgctg
     1201 tcgagtctgc ccgacgagaa gcgcggggag atgttcgcga cggcgcagtc cttgctccgg
     1261 cagcgcggat tccgggcgcg cgtggccgcc gatcacgtca tcgaccgcgg cggggttcgc
     1321 tacgtcatga ccatcatcga gatcgaacag gacgctcagg ctgaccgtga tccggccgct
     1381 tgaccgcgct tccattccgc gaagctcgag acggcctcga tgacttccat ctgctgctgc
     1441 tcgggaaacc tgttatagag gggcaggcgg agcagccggt cgctcacatc ctctgtgacg
     1501 gggcacgcgc cctcctttcc tccaaacttc cggcccatgg tggaggtgtg cagcggggtg
     1561 taatgaaaaa cgccgtggat ggatctgttc cgcaaatgcg tgatcaacgc ctgccggtcc
     1621 tcgagcgatg gcagcagcat gtagaacatg tggtaggtct gctcgcagtg tggcggcacg
     1681 accggcagcc ggacgttgtg ctccgccgcc cagaccttca ggctggtcga gtacaggttc
     1741 cacgcccggc gccgatcctc ctgcacgcgc tcccgttgct ccagttgggc gcacaggaac
     1801 gccgcgagca ggtccgacgg caggtagctc gatcccacgt ccacccagct gtacttgtcc
     1861 accagcccgc gaaagaagcg gctccggtcc gtgcccttct cccgaagtat ttcggcccgc
     1921 tcgacgagcg ctcgatcgtt gatcaggagg gctccgccct caccgcacgt gaagttcttg
     1981 gtttcgtgga aactctgtgt cgccagacac ccgaacgtcc ccaggtactt tcccttgtat
     2041 cgcccgaaca acccgtgcgc gttgtcctcg acgaccgcca ggccatggcg ggccgcgatc
     2101 tcgacgatgg catccatctc gcaccccacc ccggcgtagt ggacggggac gatgaccttg
     2161 ctccgcttcg tgatgagagg ttccagccgc gattcgtcga ggttcagcgt gtcgggccgg
     2221 atgtcggcga aaactgggcg cgcgccgcgc agcacgaacg cattcgccgt cgagacgaag
     2281 gtgaacgacg ggacgatcac ctcgtccccc ggctgcagat cgagcaggag cgccgacatc
     2341 tccagagcgt gcgtgcagga cgtcgtcagc agcgccttcg gtacgccgag tgattcctcg
     2401 agcagcgtct ggcagcgctt ggtgaatgcg ccgtcgccgc agatctgcat gttgtcgagg
     2461 gcggcgcgca tgtagtcgat ctcgctgccc aggaggtgga cacggttgaa agggatcgaa
     2521 gtcatggtgc ttccttcttg cgggccgcca gcagcagcga gccgccggcg ggaaaggaga
     2581 ctccagcagt gatcaagagc cgctcggctg ccaggatccc ctccagcgtg ccgttcaaca
     2641 gcggtccgat cctgaactcc gacagcggat cgaagtccgc ggtggcgcga ttccgctgcc
     2701 accgtgagat cagcatcagt ggcagcagta tcgacacgaa cgacgtcacc cggatcgtct
     2761 gaaagcctgc ccgctcgacc ttgtccacca gatcccttcg gccgtaccgc cgcttgtgat
     2821 gcgcgtagtc gtccatccgg ctccacagga actggtgctg cggcaccgtg agcatcacgc
     2881 cgccgccggg gcggcatgac cggaacatct cgcctagtac gcgctggtcg tcgtcgacgt
     2941 gctccagcac gtcgaacgcg ccgatcacgt cgaactccgc gtcgaacggc agtttcctgg
     3001 cgtccatctg aagcagtgtg acgcccggca ccctctgctg cgcgatgcgc aacccgcccg
     3061 tgaagatctc gctgcccgtg aggcggacgt ccgggaatcg atcgcgcatc ccggcgagca
     3121 cgaagcccgt gccgcacccg atctcgagga agctccgggc gccggggaag tagctgtcga
     3181 atgtccacga cagcagacgg ttgcgcgcgc gaaaccagaa gtggcgcgct tccacagccc
     3241 tcagctggtc gaagtagtcg gcggagaagc cgtcgttgct ctgcgcgaga tcgggcgcga
     3301 aggcgacgaa cccgtccgac tccgccggcc tccagccgca gcggccgcaa cgccagtcat
     3361 cgcggtcgaa ggttccttca cacgcgaggc agcgcttcac ggcgagatac ctcgagggct
     3421 gacatcggaa cggacggggc gagtcgccgt atcattacgc tcagcattct gtcatgggca
     3481 agctcacctt gcgggccgac gtcgccatcg gcagcctcga aaagagtcat gcggccgaca
     3541 tgctggcgtg gatgcaggac tccgagatca gcgccaacat cggcctgacc cgctcgccaa
     3601 caatggaagc caccgaggcc tggatcgcgt ccgcgttgtc ggggacatcc acgtggccgt
     3661 atgcgatctt ccacgccggc gctcacgtgg gcaacgtcgt cctcgaccag cgcgataccc
     3721 atctggggag cgcccgcctc tccgtgtacg ttggaccggc cacgtcgcgg ggccgcggtg
     3781 tgggcgtcac ggggatgtat ctcgccctgc gcgacgcgtt cgcgtcccgc gggctgcaca
     3841 aggtctggct gacagtgcac gcgcgcaaca gccgcgccat ctctgcctac acgcggctcg
     3901 gcttcgaggt ggaaggggtc ctccgcgacg gattcctgct cgcaggggag cgcgtcccgg
     3961 cgttgtacat gggcctgttg cgcggcgact ttctggccct ccagaccgaa accgtatgac
     4021 ctgcgtgatc ctgcagccct cctatattcc gtggcggggt tactttcatc agattcagaa
     4081 ggccgactat ttcgtcttct acgacgatgt ccagtacgac gcgcatggtt ggcgcaaccg
     4141 caaccggatc aagacacccc agggaccaca gtggctcacc atccccgtca ggacgaaggg
     4201 ccatctcact gagcagcgcg ccatctgcca gatggagatc gactgggatc gtccctggaa
     4261 ccggaagcat ctggcgtcgt ttcgccaggc ctaccggcac gcgcccttct acaacgagtg
     4321 ggatcccttc ctggccgagg tttacgggcg cacaccgtcg catctcgcgc cgttcaccat
     4381 cgacgtcacg atcgagctgg cgcaccggct cggcatcgcc catacccact tcctgcggtc
     4441 gtccgaactg agggtccccg gccagcgcac ggatcgcatc ctcgccatcg tgcggcacct
     4501 gggttgcgac cgctacgtca gcggaccgtc ggcgcgccgg tatctggagg aggaccgttt
     4561 tgccgacgcg ggcgtcgcac tcgagtacat gcagtacgac tatcccgagt atccgcaact
     4621 ccactcgccg ttcgacgcgg acgtgactat cctggacctc ctgctgatga cggggcccga
     4681 cgcgccgaag tacatctggg gctgacagag agacggatgc cgaagctctc catcgtggtt
     4741 ccgatctacc aggacgggta cctggtcaag cctttctgcg aggaactgca gaagcagatg
     4801 cgcctgatgc tgcgtgccga cgacatctcg cgcgatgtcg aggtgatttt cgtcaacgac
     4861 ggcagccctg acgactcaca ggccctcctt cgcgacgcgg cgcgcgcctt cccgttcgtc
     4921 aaggtcattc agctcagtcg gaacttcggt cagcacgtcg cggtgacctg cggctaccgc
     4981 ttcgcctctg gcgactacgt ggccatgatg aacgtggaca tgcaggatcc tccggaccag
     5041 atcccgcggc tgctggagag gctggagcaa ggcgactgcg acatcgtgat cggccttcgc
     5101 cagcaccgcc gcgaatcgtg gcacgaggat ctgacgtcgc acgcgttcaa cctgctgctc
     5161 aacacgctga ccgggtcccg cagcccgttg aataccgcct cgctccgtat gatgaaccgg
     5221 cggttcgtcg acgcctacaa caccctgagc gagaggacgc cgttcatacc ggggttggag
     5281 gattggctcg ggttccgccg agattacgtt cccgttcgta accaggcgag ggcgatgggc
     5341 aggtcgagtt acaccttcca caagcggctg tcgatggcgg tcgaatcgat cgtcggcttc
     5401 tcggatctgc cgctgcgcat tgccgcgtgg accggtttcg tgattacggg catcggtgcg
     5461 gtgctgaacg tctatctgct ctcccactgg ctgttcttca gggatacact gcccgggttc
     5521 acgtcgacct tctcgatcat cgtgctgctg ggtggcgcga atctgatgct tctggggttg
     5581 gtcgggcggt acgtgggccg tatcctgcgt gaagtgcagg gccgcccgcg ctacgtcatc
     5641 cagtccttcg agaatctcct accgcgtcgc gacgaaccct aagtacttga acagatggcg
     5701 ggtgagcggc aggccccgcg tcagcgtgtc cgcgcagagg tccgccgcca gcagccgctg
     5761 ctctcgctcg tagcgctcgt cgttgacgag cgcggcctgc ccgaggaacc agtagtagaa
     5821 gaactcgatc gacgcgaacc ccgccgcaat gaggcgctcc cggaggtact cctcggcgaa
     5881 tccaccggtg acgttcttgt tgtactccgc gttgttgaac acctcgcggt cgatgtcgta
     5941 cttgtcctgt atctccgtgt ccttctcgag caccgccgcc cgttcgcgct cgacgatcgg
     6001 gtcgtagacc acgcccggat ccaggctctt gaccgcgttc cagaagtcgc ggttgggctc
     6061 gagatcggcg tagaacttcc cgccccgccg cagcgcccgc gcggccgtcg ccagcgtcgg
     6121 ctcgatgtca tagaggtgat gcaggaagga gtaggcggtc accatgtcgt aggcgcccgc
     6181 ctccaccgga aaggttcccg tatcacaacg gtgcagccgg atgtccgctc cgccgctccg
     6241 gtctacctgc tggatcatcg cctcggtgat gtccacgccg tcgatttccg cgacgaactt
     6301 cttggcgatg ccgatcacga accccgtgcc gcagcccaga tccagcagct tcctcgcgcc
     6361 ggtctcggac gcgatcttcc gcagtgtgcg ctcgacgtac gcgacgtttt cgggacggaa
     6421 gtgcggctcc gtcgtgttgt agacgcccgc cagcgccgtg tgcaccttcc gattggcctc
     6481 gagcacctgc tggtaacgtg gctctgccgt ggcgtccatg ctattcgacc ttctggcggt
     6541 actttcgttc ggcgggaggc gcgtagtatt catcgtaatt ggcggggacc tgagccagaa
     6601 agcccagttt ctgcagcact cgttccgacg tgccggacag gtccatgtgc gccatggctc
     6661 gcgggatgtc gatccgctgc ttcatgtagg gacggcacat gttgatcgtc agggcgtgcc
     6721 gccatcggtc ggtttcgttg cggccgcccg catgccacac gcgcgcatcg aagtagaaca
     6781 cgcttcctgc gggcgccagc actcgctgcg ccttggcata gaactccttc tcggttggct
     6841 cgagcgcacg cgtgtgcgac ccgggcagaa accacgtggc cccgttctgc tccgtgaaat
     6901 cgtccagcag aatcgtggcg ccgacgttcg tgatgtatcc gggaatcatc ctcgggcagt
     6961 ccacatgaat tcgagtggag tagttcgtcc cgtcgggcgg catgctcgac gaggtgtagg
     7021 cgtagatgat gcaaccctct cccaggatcc attcgaaggg cgccatcagc gacggctcgc
     7081 ccaggacgtc caccagcaga cccccgtaca aggagcacag gagcaccatg ccgtcgtgcg
     7141 ctcccggata gcgccgctcc gtcagttccc gttccgttgc aatcgccgcc tcgagctcgg
     7201 cgcgcagcgg agcgaggagc gcctcgggaa tgacgcccgg cagtgtcgcg tatccctgcg
     7261 actcgatggc gcgggtgaac gcctctttgg tggtggaagc cgccatcgcc gtggtcgtca
     7321 ggtcgaagcg cctcgcgggg gcgtcgccga ccggacgatg taggccggcc gctccatcgt
     7381 tctcaggtgc atgcggccga ggtattctcc gatgatgccc agcgcgaaca gctgcgcgcc
     7441 cgagaagatg gcgatgatcg acgcgagaaa tgcgaaaccg gccaccaccg tcccggccac
     7501 gagataccgt cccaacacat aggccagtac ggccagtccg aagagcgtaa aggcgaaccc
     7561 gacgatcgtt gccagctgca gcgggatcag gctgaagccc gtcatcaggt tcatcgcgtg
     7621 ccgaaccagc gagcggaacc ggtaattgga cggaccggac cggcgagggt cgctgcgcac
     7681 cgcgacggac gcgaagctcg tggtggccca ggtgagcagc acgtcgatcg agagcaacgg
     7741 gctgcggtat tcctgaaagg cgggtttgac cgacgcccgg aacgcccgga acgggctgac
     7801 cattggagcc acggcaccgc cggtggccgt gccgagcgcg gccttggcga tgcgcgtgcc
     7861 gacgttgcgc cagagaccgt actgttgctg cgccggtctt ccatacacga cgtcatatcc
     7921 ttcggcgagc ttcgacagca ggcggggaat ctcctccgga ggctgttgaa ggtcgtcgtc
     7981 cagcgtgacg acgatgtcgt ggcggcttgc tcgaatgccg cacagcagcg cgttgtgctg
     8041 cccgtagttc cgcatgaggt cgatgccggc gatctccggg aatcggcgcg aaagcgattc
     8101 gatcgcggcc cagctccggt cacggctgcc gtcgttgatg aggatgatct cgaaggtcgt
     8161 gttcaggccc tgcagaacag ggcggagcct gtcgaggagc gccggcaggc cggcctcgct
     8221 gttgtacaca ggtatcacga ctgaaatcga ctcggacacg gctgtcaacg gtagcacggc
     8281 gtgccgagcg gttcgtaggt gcccgtgggc gtcaggccct tgtcgaccaa cacacggtag
     8341 tcgcgcatcg agtcggattt gacgggcacc tccccatagc ggctgcggac gtactcggcc
     8401 acgagtggaa aggccgcttc gtagtcgacg ctggtcttct cgatcacgat cggcacccgc
     8461 tgctgctgga gcctcgagac ggtgaggcgt tgatcctcag gcgaggcgtg ccagcccgcg
     8521 acgagatagg cctggccgcc cgcgaaacgg cgttcggtcg tgaagtacac gtcgggcccg
     8581 aaccacgtca cgaagagccg gtccgtgtcg gccgtgcact cgaacacata tcgcatgagg
     8641 cccccgagac cggaagcctc gcgcggccac gcgtcgatgg gccgcgcctg gagcagttcg
     8701 gccgtcgcga tcacccggcg accgatgccc tcgctgagaa tccggctggc tctcacattc
     8761 gccatcgtct ccgcgaatgt cccgacgctc cacgccgtga tcacgatccc ggcggcgacg
     8821 agagtcattg tcgcgcggcg cacccatggc tttgctccga cggccgcccg aagacatctg
     8881 gcgctcaccc aggctgccac gacggcaatc gggttcgcca cgtcgggaag ccgcgaatcg
     8941 ggcgaaccgc gcacgagcgt ctggacaag
//
LOCUS       DBNGSH010000577         3150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2774559, whole genome shotgun sequence.
ACCESSION   DBNGSH010000577 DBNGSH010000000
VERSION     DBNGSH010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2774559"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="AC16CY_13350"
     CDS             <1..498
                     /locus_tag="AC16CY_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354827.1"
                     /translation="SADSLASGGDDPDGILPLRALTWIGIFGYPPARVDHRFKDGDTI
                     RVGPIALTAHITAGHTRGCTSWSFPVRDGDRVLNVVSACDLGVLATSRYPEQAADRER
                     SIRVLRSLPADIWVTCHARWWGRYRKFVASTTAKNPVDPFIDPEGYHAYLDAAEAELR
                     QGRVH"
     gene            763..1383
                     /locus_tag="AC16CY_13355"
     CDS             763..1383
                     /locus_tag="AC16CY_13355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3354828.1"
                     /translation="MLYVSDIHLRNGRSDRLCTQVLDAVAGCRPDVVLLGGDLVDSSS
                     ELARLSDLVGRLREIASVLAIGGNHDRRIGMDRVREAVEHGGGHWIHDATARVTHGGR
                     VIAVSGPDAAGPGDGDVRVLCAHNPHIWKTCHHAGYDLVLAGHLHGCQIVACVYRDRL
                     FPGAMIYPYCFLSHHRDSTRLVVSRGVSDLLPIRWRCPREVVLCYV"
     gene            1449..2198
                     /locus_tag="AC16CY_13360"
     CDS             1449..2198
                     /locus_tag="AC16CY_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354829.1"
                     /translation="MSITCLLRGVPLFFSAAPRTPLRVLCIVALDTIHVLRHSQSLSR
                     QRRQELATFLDFQACTNAVWDRKPLCAEEYQAQRQRLEKAGLGPWIAEFVVRLRELET
                     RRPPAGGDRRCFDAVRSYREAVARLSLATITAIALNAKGVDEGIRATHGDGDVAALFR
                     MAMQCQIIDDVVDYEEDLSAGLPSFLTASASLLQALSWTAEAARSYALVPGHSAGPSV
                     LPVRVALHLVTTVTKLVIAVAGRGESRVSGG"
     gene            2207..2566
                     /locus_tag="AC16CY_13365"
     CDS             2207..2566
                     /locus_tag="AC16CY_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3354830.1"
                     /translation="MSAQTLALILAAIAFSATGQLLLKSGAQHLAGLGRLEFLLAAAR
                     DVRVLAGLAAWVAWTMCWLHVLRVAPLSRAYALTSLTYVLIPLASIYVFGEQVRRLHV
                     AGIILIAAGIACVLADG"
     gene            2559..>3150
                     /locus_tag="AC16CY_13370"
     CDS             2559..>3150
                     /locus_tag="AC16CY_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR3354831.1"
                     /translation="MVERARELAARAYFGLCLASGRMVRSATYSKVWVVREGGQRVVR
                     KRRLFYAPLLIWMSGLLVWILDAGVRVLRQRDWEERERRMYGSLRGSSIAAHADGVLV
                     LPFLAGDTLATLLEDPQLEESSRKRAIEHATVALAEFHRLGFTHADAMAENVMVDLDA
                     GVARWFDFETLHDASRPLTWQRADDVRALLATCLLRT"
BASE COUNT          492 a         1015 c         1062 g          581 t
ORIGIN      
        1 agcgccgact ccctcgcctc cggcggcgac gaccccgatg ggatcctgcc gttgagggcc
       61 ctcacctgga tcgggatctt cggctatccg cccgcgcgcg tcgaccaccg gttcaaggac
      121 ggcgacacga tccgtgtggg gccgatcgcg ctcaccgcgc acatcacggc cggtcataca
      181 cgcggctgta cgtcgtggtc attccctgtc cgcgacggtg accgcgtgct gaacgtcgtc
      241 agcgcgtgtg acctcggagt gctggcaacc tcgcggtatc cggaacaagc ggcagaccgc
      301 gagcgcagca tccgcgtgct ccgcagcctc ccggccgaca tctgggtgac gtgtcatgca
      361 cgatggtggg gcaggtaccg caagttcgtc gcgagcacca cggcgaagaa ccctgtagac
      421 ccgttcatcg accccgaggg ctaccacgcc tacctcgacg ccgccgaagc cgaattacgc
      481 caagggcggg tgcactagca cccgcggcac agttcgcatc ggcgaaaggc ggcatctcat
      541 tcgaatctca cgagccgctc acgaaccggt gaggacttcc accgcgcgct ccgtcacagt
      601 caagggctac gttcagaggc cgtgcatttg atcggaagcg cgaggtgtca gcagctctcg
      661 aactcgagac gggcgtggtc cggcggagcg ctggaaatcg gactgcgtcg agccttgcac
      721 gttcgcgagg agcgtctcgc gacgcggcac gacgcgtatc gaatgctgta tgtcagcgac
      781 atccacctcc gaaacggtcg ctccgacagg ctatgcacgc aagtcttgga tgcggtcgca
      841 ggctgccgtc ctgatgtggt gctgctgggc ggcgaccttg tggacagttc ctccgaactg
      901 gccaggctga gtgacctggt tggcaggctc cgcgaaatcg catcggttct tgcgatcggc
      961 ggcaaccatg accggcggat tggcatggat cgcgtccggg aggcagtcga gcatggcggc
     1021 ggccactgga tccatgacgc cacggcgcga gtcacgcatg gcgggcgggt gatcgcggtc
     1081 tcaggccccg acgcagcggg ccctggcgat ggggatgtcc gtgtactgtg cgctcacaat
     1141 ccgcacatct ggaagacatg ccaccatgcc ggctacgacc tggtcctggc cggacacctg
     1201 cacgggtgcc agatcgtcgc gtgcgtgtat cgagaccgcc tgtttccggg cgcgatgatc
     1261 tacccctact gcttcttgag tcatcaccgc gattcgacgc ggctcgtggt cagccgcggc
     1321 gtgagtgatc tcctgccgat tcggtggaga tgtccgcgcg aggtcgtgct gtgctacgtc
     1381 tgaccggagc cgagcgaggc gacacacgcg cccgacgcgt tcctcctgga ccgctgcgtg
     1441 ccgggctcgt gtccatcacg tgcctgctcc gcggtgtgcc gctcttcttt tccgcggcgc
     1501 cgcgaacgcc gctacgagtc ctgtgcattg ttgcgctcga caccattcac gtgcttcgac
     1561 actcgcagtc gttgtcacga caacgccgtc aggagttggc aacgtttctg gattttcaag
     1621 cgtgcacgaa cgcggtgtgg gaccgcaagc cgctgtgcgc ggaggaatat caggcgcagc
     1681 ggcagcgttt ggagaaggcc ggcctcggac cgtggatcgc agaattcgtc gtgcggcttc
     1741 gtgagctgga aacccgacgc ccgccagccg gcggggatcg gcggtgcttc gacgcagttc
     1801 gttcgtaccg cgaggcagtc gcgcggctgt cgctggcaac gattacagcg atcgcgctca
     1861 acgccaaagg tgtcgacgag ggaatccggg cgacgcatgg cgatggcgac gtggcagcgc
     1921 tgtttcgaat ggcgatgcaa tgccagatca tcgacgatgt cgtcgattac gaagaagacc
     1981 tgtcggcggg cctgccgagt ttcctgaccg catccgcgtc gctgctgcag gcgctgtcgt
     2041 ggactgccga agcggcacga tcctatgccc tggtccccgg gcattccgca ggtcctagtg
     2101 tgttgccggt gagagtggcg ctccatctgg tgaccacggt gacgaagctc gtcattgccg
     2161 tcgcgggaag aggagagtcc cgcgtttcag gaggatgacc ctggcgatgt cggcacaaac
     2221 gttggcgctc atcctggctg cgatcgcgtt ctcggcgacg ggacagctcc tcctgaagtc
     2281 gggtgcccag catctggccg gcctcgggcg cctcgagttc ctgctcgccg ccgcacggga
     2341 cgtacgggtc ctggcggggc tggctgcctg ggtcgcctgg acgatgtgct ggctccacgt
     2401 cctcagagtc gcgccgttgt cgagagccta cgcgctgacc agcctcacct acgtgctcat
     2461 tcccctcgcc agcatctacg tcttcggcga acaggtccgc cgtctgcacg tcgctggcat
     2521 cattctgatc gccgcgggta tcgcgtgcgt gctcgccgat ggttgagaga gcgcgggagc
     2581 tggcggcgcg tgcctacttc ggcctgtgcc tcgcctcggg gcggatggtt cgctccgcga
     2641 cgtactcgaa ggtgtgggtg gtccgcgagg gcggccagcg tgtcgtgcga aagcgccgcc
     2701 tcttctacgc gccgctcctg atctggatga gtggcctgct ggtctggatc ctggatgccg
     2761 gcgtgcgggt cctgcgccag cgggactggg aggagcgcga gcgccggatg tacgggagcc
     2821 tccgcggcag ctcgattgcc gcccacgcgg acggggtgct cgtcctgcca tttcttgctg
     2881 gagacacgct ggcgacgttg ctggaggatc cccagctgga ggagtcgagc cgaaagaggg
     2941 cgatcgaaca cgccaccgtc gccctcgccg agttccatcg cctgggattc acccacgccg
     3001 atgcgatggc ggagaacgtc atggtggatc tcgacgccgg cgttgcccgt tggtttgact
     3061 tcgaaacgct tcacgatgcg agtcgtccgc tgacctggca acgtgcagac gacgtgcgag
     3121 cattgctcgc cacgtgcctc ctccggaccg
//
LOCUS       DBNGSH010000578         6397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2265351, whole genome shotgun sequence.
ACCESSION   DBNGSH010000578 DBNGSH010000000
VERSION     DBNGSH010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2265351"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="AC16CY_13375"
     CDS             complement(<1..756)
                     /locus_tag="AC16CY_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HIR3354832.1"
                     /translation="MQRSIPCTMMRGGTSKGAYFVASDLPSDPPARDRVLLAVMGSPD
                     PRQIDGVGGADPLTSKVAIVSPSSKPGIDVDYLFCQVWVDKPEVSTAQNCGNILAGVG
                     PFAIERGLVRGCDPVTKVRIFMVNTSQTVVATIQTPGGRPEYQGDARIDGVPGTAAPI
                     PLSFSDTAGSTCGALLPTGHASDVIDGVQVTCIDNGMPIVAMRAADLGRTGYESREQL
                     DGDVELKARVERIRLLAGPMMNLGDVKDQTIPKM"
     gene            complement(792..1775)
                     /locus_tag="AC16CY_13380"
     CDS             complement(792..1775)
                     /locus_tag="AC16CY_13380"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HIR3354833.1"
                     /translation="MQSTIRSFDLAHLGHLEILTPKPDESLHFFVNVMGMTETTRRGD
                     SVYLRGWDDYEHHTLKLTAAKVAGMGHFAFRTTSPEALQRRVAIIEKGGTGKGWTDGD
                     LGHGPAYAFTTPDGHNMELYYETEWYKAPPELKPSLKNQAMRFPARGANVRRLDHLNL
                     LTSDVKGLQRFMEDTLGMRTTEMIVLDSGAIAGVWVTCNNKTYDVAFTADHYGAHGRF
                     HHATWAVDSKDEVLRAADICLENGIYIETGPHKHAVQQTFFLYVYEPGGNRIEFDSAG
                     ARLMLAPDWQPIVWTEAERKKGQAWGLKTIDSFHTHGTPPIEQYTGPRTKQ"
     gene            complement(1778..2599)
                     /locus_tag="AC16CY_13385"
     CDS             complement(1778..2599)
                     /locus_tag="AC16CY_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354834.1"
                     /translation="MSARLASVTARMTAAAVCAVIVSVVVQPEAAQRGRGQGGPVPTG
                     RLGAPVDLTGNWVSLVTEDWQWRMRTPPKGDYVSVPMSGRGRQLADTWDASKDGMCEA
                     YGVGGLMRMPLRLRISWQDDTTLKIETDAGQQTRLLRFAAQGPAAQTASNTAPQARTL
                     QGSSAAEWVRSGGAFDAFLERGAGAPPPRWGSLKVVTTNVLPGWLRRNGVPYSQDAVI
                     TEYFTRITHPVAGDIFVVTTTVEDPAMLTQPFTTSSNFKKEPDGSKWSPAPCRTS"
     gene            complement(2596..3666)
                     /locus_tag="AC16CY_13390"
     CDS             complement(2596..3666)
                     /locus_tag="AC16CY_13390"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354835.1"
                     /translation="MRFAHTAPSRTLRSLSAAAAVVAGAALAFGSGAVAQQNQDFDKV
                     QIETVRVRDNVHMLVGAGGNTTIFTGSDGIMVVDTQFAPLGTKILSAIRAISTAPIRY
                     VVNTHLHPDHIGGNEAISKAGRFRAGGNVVGDLGAAATATAVIIAHENVLRRLSKEPA
                     PGQTALPFAFLPTETYITKRREFLFNGEAVQIIHEPKAHTDGDSVVFFRKSDVVATGD
                     LFTTVMYPYIDVANGGTIDGYINALNAIMDITVASNVNEGGTLVIPGHGRVCDEQDVV
                     EYRNMATIIRDRIKEYVKRGMTLEQVKAARPTLDFDPRYGSDTGFWTTSMFIETIYKQ
                     MVEANPPPKPAQKGSQRKGGAE"
     gene            complement(3668..4594)
                     /locus_tag="AC16CY_13395"
     CDS             complement(3668..4594)
                     /locus_tag="AC16CY_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354836.1"
                     /translation="MKKLLTVAAVVLAAANTPAFAQAGADFSGVWQPRYHEDQPDRIP
                     GPELTDYTGLPINDAARQYADSWDSSRITLPEEQCRVHVSPYIYRGPLNLRIWEEKDP
                     KTQDLIAIKHYSSTFEQTRTIWMDGRPHPGPNAPHTWMGFSTGRYDGDMLVVETTHLK
                     KGWVRRNGVPMSDQARMTDYFVRNGDMMSHITILIDPVYLSEPLIRSQEFFRSQRELP
                     AGTWLWVCDPVLEIATREEGEVPAYLPGEHPFKEEYARRHQLPLIAVRGGAATMYPEF
                     QEVMKKAPIPPPLPPRQQGATRVTGPGQQQGR"
     gene            4759..5691
                     /locus_tag="AC16CY_13400"
     CDS             4759..5691
                     /locus_tag="AC16CY_13400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3354837.1"
                     /translation="MHPSDVPDLRARELLTVIALAEYGSFVAAAAYLKTSQPALTRTI
                     KRVERVLGVTLFARNTRRVEITAAGREFVAVAERMLNELHLTVRNLSEVTNEQRGRVT
                     VSTYSAFAVNPLPFLVRRYRETRPLIEVRIREGRQSEIVEDVRNGAADFGIGYVNALP
                     DMLQATTLRKEPLHVLIPPAHEFAVKKRQRVRLADLRDEALVSAPSDTYLRRVVDAAA
                     MSAGFSLHYRVVVDRLLSIVHHVSAGVAIGILPSGCIPPIRWGEGFHSAVLVEPSLTV
                     TVGLITSRSRYLTPAASGLAALIKEQISSKQPKA"
     gene            complement(5701..>6397)
                     /locus_tag="AC16CY_13405"
     CDS             complement(5701..>6397)
                     /locus_tag="AC16CY_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354838.1"
                     /translation="RFPSVAAQFADAQPIREFTWMPRLPYRAAVATGERWAMLPSAAA
                     FIDPLFSTGIPLTLLGIERLARMLEGGTAGEGGSKDPPDRWRAYNEATLADADLTARF
                     IAGSYAGFGRFGAFAAYSMFYFAAASFSEMARRLSSPRTPRGFLCGDSHAFTSALERL
                     SPAVHTAADIGRYEDDVAAAVSDRNIAGLCDRGKQNWYAVDLEDTIRAAGKLGLTPDE
                     VRDALVPEPLRSR"
BASE COUNT          960 a         2125 c         2170 g         1142 t
ORIGIN      
        1 catcttcgga atcgtctgat ccttcacgtc gccgaggttc atcatgggtc ccgcgagcag
       61 gcgaatgcgc tcgaccctgg ctttgagctc gacgtcgcca tcgagctgct cgcggctctc
      121 gtagcccgtg cggccgaggt cggcggcgcg catcgcgaca atcggcatcc cgttgtcgat
      181 gcaggtgacc tggacgccgt cgatgacgtc gctcgcgtgg ccggtcggca ggagcgcgcc
      241 gcaggtggat ccggccgtgt ccgagaagct gagcggaatg ggggccgccg tgcccggcac
      301 gccgtcaatc cgcgcgtcgc cctggtactc gggcctgccg ccgggcgtct ggatcgtcgc
      361 cacgacggtc tgcgacgtgt tgaccatgaa gatgcggacc ttcgtgaccg ggtcgcagcc
      421 acgcaccagc ccgcgctcga tggcaaacgg cccgacgccc gccaggatgt tgccgcagtt
      481 ctgtgcggtc gagacctcgg gcttgtccac ccacacctgg cagaacaggt agtcgacgtc
      541 gataccgggc ttcgaggagg gcgagacgat cgccaccttg ctggtgagcg gatcggcgcc
      601 gcccacgccg tcgatctggc gcgggtccgg cgaccccatc acggcgagga gcacgcggtc
      661 ccgcgccggg gggtcagacg gcagatcgga ggcgacgaag tacgccccct tggaagtgcc
      721 gccgcgcatc atggtgcagg ggatggatct ctgcatgcct gccaggcggg tctaaagacc
      781 cgcctctact gctactgctt cgtcctcggt ccagtgtact gctcgatcgg cggcgtgccg
      841 tgcgtgtgga agctgtcgat cgtcttgagt ccccacgcct ggcctttctt ccgctcggcc
      901 tcggtccaca cgatgggctg ccagtcgggc gccagcatca gccgggcccc ggcgctgtcg
      961 aactcgatgc ggttgcctcc gggctcgtac acatagagga agaacgtctg ctgcacggcg
     1021 tgcttgtgcg gaccggtctc gatgtagatg ccgttctcga ggcagatatc ggcggcccgg
     1081 agcacctcgt ccttcgaatc gaccgcccac gtggcatggt ggaaccgccc gtgggcgccg
     1141 tagtgatcgg cggtaaacgc gacgtcatag gtcttgttgt tacaggtgac ccagacgccg
     1201 gcgatggctc cgctgtcgag cacgatcatc tcggtggtgc gcatgcccag cgtgtcttcc
     1261 atgaaccgct ggagtccttt gacgtcggac gtcagcaggt tcaggtgatc cagccgccgg
     1321 acgttggcgc cacgggccgg gaagcgcatc gcctgattct tgagcgacgg cttcagctcg
     1381 gggggcgcct tgtaccactc ggtctcgtaa tagagctcca tgttgtggcc gtcaggcgtg
     1441 gtgaaggcat aggccggccc gtgaccgagg tcgccgtcgg tccatccctt tccggtcccg
     1501 cccttctcga tgatggccac gcgccgctgc agcgcctcgg ggctcgtggt gcggaacgcg
     1561 aaatggccca tccccgccac cttcgcggcc gtcaacttga gcgtgtggtg ctcgtagtcg
     1621 tcccagcctc gcaggtacac cgagtctccg cggcgagtcg tctcggtcat gcccatgacg
     1681 ttgacgaaga agtgcaggct ctcgtcaggc ttgggcgtca ggatctccag gtgcccgagg
     1741 tgcgcaaggt caaaggatcg gattgtggac tgcacggtta cgaggttcta cacggagccg
     1801 ggctccactt cgacccgtcc ggctctttct tgaaattcga gctggtggtg aacggctgcg
     1861 tgagcattgc gggatcctcg acggtcgtcg tcacgacgaa gatgtcgccg gcaacggggt
     1921 gcgtgatccg cgtgaagtac tcggtgatga cggcgtcctg gctgtacggc acgccgttgc
     1981 ggcgcagcca gccgggcagg acgttcgttg tcacgacctt gagcgagccc catcgcggag
     2041 ggggcgcgcc ggcgccccgc tcgaggaacg cgtcgaaggc gccgccgctg cggacccatt
     2101 cggcggccga ggagccctgc agcgtccggg cctgcggcgc ggtattcgac gccgtctgtg
     2161 ccgcggggcc ctgggccgcg aagcgaagaa gccgcgtctg ctggccggcg tcggtctcga
     2221 tcttgagcgt cgtgtcgtcc tgccagctga tgcgcagccg caagggcatc cgcatgaggc
     2281 cgccgacgcc gtaggcctcg cacatgccgt ccttcgacgc gtcccacgtg tcggccagct
     2341 gccggccgcg tcccgacatc ggcacgctga cgtaatcgcc cttcggcggt gtgcgcatcc
     2401 gccactgcca gtcctcggtc acgagcgaca cccagttgcc ggtgaggtca acgggcgcgc
     2461 cgagacgccc tgtcggcacc ggccccccct gtcccctgcc gcgctgcgcc gcttcgggct
     2521 gaaccaccac cgagacgatg acggcgcaca ccgccgccgc cgtcatgcgt gccgtcacac
     2581 tcgcgagacg cgcgctcatt ccgctccccc tttgcgctgg cttcccttct gcgccggctt
     2641 cggcggcgga ttggcttcga ccatctgctt gtagatcgtc tcgatgaaca tcgaggtcgt
     2701 ccagaagccg gtgtcggagc cgtagcgggg atcgaagtcg agcgtcggcc tggcggcctt
     2761 cacctgctcg agcgtcatgc cgcgcttgac gtattccttg atgcggtcgc gaatgatcgt
     2821 cgccatgttt cggtactcga cgacgtcctg ctcgtcgcac acccggccgt gccccgggat
     2881 gacgagcgtg cccccctcgt tgacgttgct cgcgacggtg atgtccatga tggcgttcag
     2941 cgcgttgatg tacccgtcga tcgtgccgcc gttggcgacg tcgatgtagg gatacatcac
     3001 ggtcgtgaac agatcgccgg tcgccacgac gtcggacttg cggaagaaga ccacgctgtc
     3061 gccatcggtg tgggctttcg gctcgtggat gatctggacg gcctcgccgt tgaacaggaa
     3121 ctcgcgccgc ttggtgatgt aggtctcggt cggcaggaac gcaaacggca gcgccgtctg
     3181 ccccggcgcg ggctccttgc tcagccgcct cagcacgttt tcgtgcgcga tgatgaccgc
     3241 cgtcgccgtg gccgcggcgc cgagatcgcc cacgacgttg ccgccggcgc ggaagcgccc
     3301 ggccttggag atcgcctcgt tgccgccgat gtgatcgggg tgcaggtgcg tgttgacgac
     3361 gtaccggatc ggcgcggtgc tgatggcccg gatggccgag aggatcttcg tgccgagcgg
     3421 cgcgaactgc gtgtctacga ccatgatgcc gtcggaaccg gtgaagatcg tcgtgttgcc
     3481 gccggcgccc accagcatgt gcacgttgtc gcggacgcgg acggtttcga tctggacctt
     3541 gtcgaaatcc tggttctgct gcgccacggc tccagagccg aacgcgagcg ccgcgccggc
     3601 gacgacggcc gccgcggcag acagggaccg cagggtacgc gagggagcag tgtgggcgaa
     3661 tctcatgtca gcggccctgc tgctggcccg ggccggtcac gcgggtcgcg ccctgctgcc
     3721 gcggaggcag cggcggtgga atgggcgcct tcttcatgac ctcctggaat tccggataca
     3781 tcgtcgcggc gccgccgcgg acggcgatca ggggcagttg atgacggcgc gcgtactctt
     3841 ccttgaacgg gtgctcgccc ggcaggtagg ccggtacctc gccttcctcg cgtgtggcga
     3901 tctcgagcac gggatcgcag acccagagcc acgtgcctgc cggaagctca cgctggctgc
     3961 ggaagaattc ctggctcctg atgagcggct cggacagata caccgggtcg atgaggatgg
     4021 tgatgtgcga catcatgtcg ccgttgcgga cgaagtagtc ggtcatcctc gcctggtcgc
     4081 tcatcggcac gccgttgcgg cggacccagc ctttcttgag gtgcgtcgtc tcgacgacga
     4141 gcatgtcgcc gtcgtagcgc cctgtcgaga agcccatcca cgtgtgcggc gcgttcggac
     4201 ccggatgcgg ccggccgtcc atccagatcg tccgtgtctg ctcgaacgtg ctgctgtagt
     4261 gcttgatggc gatgaggtcc tgggtcttcg gatccttctc ttcccagatg cgcaggttga
     4321 gcggcccccg gtagatgtag ggggagacgt gcacacggca ctgctcttcc ggcagcgtga
     4381 tgcgcgacga gtcccagctg tcggcgtact ggcgggccgc gtcgttgatc ggaaggcccg
     4441 tgtagtcggt cagctcgggg cccggaatgc gatcgggctg atcctcgtgg tagcgcggct
     4501 gccagacgcc cgagaaatcg gcgcctgcct gcgcgaacgc cggcgtgttc gccgcagcga
     4561 gcaccacggc tgccacggtc agtagttttt tcataggggg tcgggagtca ttttctcgag
     4621 gacatcctcg cgcggagtcc ggggagcgtc ctcgagaaaa tgactcccga ccccattttc
     4681 accaacagcc tattctacat gcgacccgac tgcgactaga attgcggacc gcgaataagt
     4741 cccccggagt catccgcaat gcacccttcc gacgtaccgg accttcgagc gcgggaactg
     4801 ctgaccgtca tcgcgctcgc cgagtacggc agcttcgtcg cggcggccgc ctacctcaag
     4861 acgtcccagc ccgcgctgac acgaacgatc aagcgggtcg agcgcgtgct gggtgtcacg
     4921 ctcttcgccc gcaacacgcg ccgcgtcgag atcacggcgg cgggccgcga attcgtcgcg
     4981 gtggccgagc ggatgctcaa cgagctacat ctcaccgtac gaaacctcag cgaggtgacc
     5041 aacgaacagc gcggacgcgt caccgtctcg acctattcgg cgttcgcggt gaatcccctc
     5101 ccgtttctcg tccgccgtta tcgcgaaacc aggccgctga tcgaggtgcg catccgcgag
     5161 ggccggcagt cggagatcgt ggaggacgtg aggaacggcg ccgccgattt cggcatcggc
     5221 tacgtcaacg cgctgccgga catgcttcag gcgacgacgc tgaggaagga gccgctgcac
     5281 gtcctgattc ctccggccca cgagttcgcc gtcaagaaac ggcagcgcgt gcgcctggcc
     5341 gatctgcgcg atgaagcgct cgtctcggcg ccgagcgaca cctacctgcg gcgtgtcgtg
     5401 gacgcagccg ccatgtcggc cgggttcagc ctgcactacc gcgtggtcgt cgatcgcctg
     5461 ctcagcatcg tccaccacgt gtcggccggt gtcgcgatcg gcattcttcc ttccggctgc
     5521 atcccgccga tccggtgggg tgaagggttc cactcggccg tgctggtcga gccgtcgctg
     5581 acggtgaccg tgggcctcat cacctcgcgc agccgctacc tgacgccggc cgcctcgggc
     5641 ctggcggcgc tgatcaaaga acagatctcg tcgaagcaac cgaaggcgta gtgtccggct
     5701 tcagcgggac ctgaggggct caggcacgag cgcgtcgcgt acttcgtccg gcgtgaggcc
     5761 cagcttgccg gcggcgcgga tcgtgtcctc caggtctacg gcgtaccaat tctgcttgcc
     5821 gcggtcgcag aggcctgcga tgttccggtc gctgacggcc gccgccacgt cgtcctcgta
     5881 gcgcccgatg tctgcggccg tatgcaccgc cggcgacagg cgctcgaggg cggacgtgaa
     5941 cgcgtggctg tcgccgcaca agaagcctcg cggtgtgcgg ggagatgaca gccggcgggc
     6001 catttcgctg aagctcgccg cggcgaagta gaacatcgag taggcggcga aggcgccgaa
     6061 ccgaccgaat cccgcgtagc tgccggcgat gaagcgcgct gtgagatcgg cgtcggccag
     6121 cgtggcctcg ttataggcgc gccaccggtc aggcgggtct ttagacccgc cttcccccgc
     6181 cgtcccgcct tccaacattc gcgccaaccg ctcgatcccg agcagcgtga gcggaatccc
     6241 cgtggagaag agcggatcga tgaatgccgc cgccgatggc agcatggccc atcgctcccc
     6301 cgtcgccaca gcggcgcggt agggcaggcg cggcatccag gtgaactcgc gaatcggctg
     6361 cgcgtcggcg aactgtgcgg ccacggacgg aaatcga
//
LOCUS       DBNGSH010000579         6915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4302322, whole genome shotgun sequence.
ACCESSION   DBNGSH010000579 DBNGSH010000000
VERSION     DBNGSH010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4302322"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="AC16CY_13410"
     CDS             <1..906
                     /locus_tag="AC16CY_13410"
                     /inference="COORDINATES: protein motif:HMM:NF047558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3354839.1"
                     /translation="PESLDRAQKLADQGLALMKENPLLLATRGMVSWYYLNFSIRPEE
                     RYLDEAASFATRALDQDPQAFLGIFLRGLVAAKRGDIEAAVGDIRRANDLKPGDLAYL
                     ELMRWLFSAGHPSETVFEAGARIDPLNPLYWTQLALARFVQGRIAEAEYAARRGIELS
                     DRGTPVRFYAAIGLVLAGRQEEGIGVLDEVASTLGESPYGSISAFMARALQGDANAAS
                     RHVTPLVEQSARWVEYLALFLADGYALIGRRDDALRWLKEAVRLGFINYPFFTRDPFL
                     ESLRGDAEFQALMQQLERRWRSFPV"
     gene            940..2148
                     /locus_tag="AC16CY_13415"
     CDS             940..2148
                     /locus_tag="AC16CY_13415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIR3354840.1"
                     /translation="MAHVVRPPRSIRVAAAAGVRAARRLVMTVDLLAPPWEKCKPNAM
                     SPLFGPLVFRTGLSARNRLVLAPLTNMQSHADGSLGDDELRWLTSRAEGGFGLVMTCA
                     AHVARDGQGWRGELGVFDDALLPGLTRLATALGEHGAASLVQLFHGGLRADPSLTGLV
                     PWSASEGDGCRAATEQDLTRVIAQFSAAARRSQEAGFDGVELHGAHGYLFTQFLSVTQ
                     NRRLDQWGGTLENRARLLRETVRAVRAAVGASFTVGVRLSPEDFGNARGLDLDESVQV
                     AEWLADDGVDFVHLSLWQALASTAKRPEANAIPLFRSAVPSDVRVLAAGGIWTRAEAE
                     EVLRRGADAVVLGRSAIVNADWPRRAADPGWAPRRPPVTIDSLVASGLSRPFAEHMRR
                     FKDFVVEESR"
     gene            2155..3192
                     /locus_tag="AC16CY_13420"
     CDS             2155..3192
                     /locus_tag="AC16CY_13420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354841.1"
                     /translation="MAAVYKIGAMTISRRSAIQRVVAAAGFTIGPLTIPRRARLQAPS
                     TTPGARLVLLGTQGGPNYTATRGEAANAVVVDGQPYLVDCGYGALAALKKAGVNHRQI
                     ARIFLTHLHDDHTADLAALLNRQWTDGRVDPVVVAGPFGTARLVEAVIALGEANAAIR
                     LADEARSVKPAEMFRGQDLAATAAPVEVYRDERVTVRAVENTHFPAESKAKMPYRSLS
                     YRLDAGGRSFVFSGDTAYSPGLVALARGAEILVCEGLDIAAMRKAFDGMVARGMYADN
                     PEGVWQHIVGTHTPLEDAGRMAAEAGVRTLVLTHLVPGALDPLPDETYLTPARKTFRG
                     QVVLGADQLVL"
     gene            complement(3277..5202)
                     /locus_tag="AC16CY_13425"
     CDS             complement(3277..5202)
                     /locus_tag="AC16CY_13425"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3354842.1"
                     /translation="MKSALTVAFVLSACVSFAQAPGPRGDLIRTTLPLDGAPKAIPGP
                     YKVMSEPALGSPGHVVFRPEDLGRFPGRDKLPVMAWGNGGCAINSARYSGLFTTIASH
                     GFLVIGSAPEPGAATRQQNDDDLRKAIAWAEKENARDGSPLKGKVDLEHVAVMGQSCG
                     GFLSIALGSDPRVKTIGVFNSGVQRARPDSNDDALQKVHGPVLLVNGAERDLLTPASL
                     TTFELIKNVPAFYGARHGAGHTATVDHPGGGEYANVASNWLLWQFKNDKKAAAMFVGD
                     QCELCTNSNWDVRAKGYKDARNEGPSGTFDRGSSQQAWQHANYRTTLSQCKNPPQPFA
                     IPSAGNAATATAAPPEPVLPPTSSIPGVLEAVQSWKVVWSWQGNNADGPIAGENGTML
                     FANNDAGNVIQFDPATNLAKVAYGNVNTAGAVSRSKNGALFVAERGLGGGIAQLGPQR
                     RMLANTFNGEPLECIGGVINDLTADASGGVYFSVTGARNSGVFYASPKGVVSQYGKDV
                     PAANGIILSPDEKTLYVTNGATVFAFDVNADGSLTNQREFGKLQGGEGGDGSAVDQQG
                     RLYVATGRSVDVFAADGKFLGTIPGPQGLHGTFFGGRDRRTLYGIVFYGTWGTPSARN
                     EIIAIPTIAQGYTGRAK"
     gene            5304..6914
                     /locus_tag="AC16CY_13430"
     CDS             5304..6914
                     /locus_tag="AC16CY_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3354843.1"
                     /translation="MGVHSVRSHGSIRVAAAALAVSVCVAAHLTGQTTAVDWPSYNRT
                     FTSERYAPLDQIDRTNVSRLRQVCVYDLEVDTNFQTGPIVIGGTLYATTDREILALDA
                     ATCQPKWRVREPGPSVGLAVNRGAAYLDGRLFRGTPDGDLAAYDAQTGKRLWTTRLAD
                     REKGESVPSAPIAWNGMVFIGTAGGDVYGVKGRMYALDAGTGKVLWETYTVPTDAPQP
                     GNEKMQAQAKVTWGNEPGVPITGGGTWSSYSLDTQRGLLYLPTGNPAPDFATDVRPGD
                     NLYTNSVLVLDAKTGIYRNHYSLVPADFHDWDLSAAPVLVTTRGGKRIVAGAPKDGLL
                     HAYDVATDKKLYATPVTTRENENAPLSTTPTRFCPGAAGGTEWNGPAYSPDTNFFYSG
                     TVDWCVTVTLDPSELAKKPGQAWTGTQLSAQFGVRDLNWAGWVTAVDADSGQVKWRVR
                     AGAPVVSGVTPTKGGLVFAGDMDKRAYAFDAETGTILWRSDLPGAPGGGVITYAVGGK
                     QYVVFVAGTRSRVFPVSAASAKIVVFGL"
BASE COUNT         1036 a         2346 c         2383 g         1150 t
ORIGIN      
        1 cccgaatcgc tggaccgcgc gcagaagctc gcggaccagg ggctggccct catgaaagag
       61 aatccgctgc tcctggcgac gcgcgggatg gtttcgtggt actacctcaa tttctcgatc
      121 cgccccgagg aacggtacct cgacgaggcg gcgtcgttcg cgacccgcgc actcgatcag
      181 gatccccagg ccttcctcgg aatcttcctc cgcggcctcg tcgcggccaa gcgaggggac
      241 atcgaggcgg cggttggcga catcaggcga gccaacgatc tgaaaccggg ggacctggcg
      301 tacctcgagt tgatgcgctg gctcttctcg gcaggtcacc ccagtgagac ggtgttcgag
      361 gcgggcgcgc ggatcgatcc gctcaacccg ctctactgga cgcagctcgc cctggctcgt
      421 ttcgtccagg gcagaatcgc cgaagcggaa tacgcggcgc gccgaggtat cgagctctcc
      481 gatcgcggga ctcccgtccg gttctatgcg gcgataggcc tggtgctcgc cgggcggcag
      541 gaggaaggga ttggcgtgct cgacgaggtc gcctcaacgc tcggggagag cccgtatgga
      601 tcgatcagtg ccttcatggc ccgggcgctg cagggcgacg ccaacgccgc ctcccgccat
      661 gtcacgccgc tcgtcgagca gtcggcgcgc tgggtggaat acctcgcgct gtttctcgcc
      721 gacggctacg ccctcatcgg ccgtcgcgac gacgcgctcc gctggctgaa ggaagcggtc
      781 cggctgggat tcatcaacta cccgttcttc acgcgcgatc cgttcctcga gtcgctgcgc
      841 ggcgacgccg aattccaggc gctgatgcag cagctcgagc ggcgctggag gtcgtttccg
      901 gtctgacggg tcggcgccat tccaacgagg aggacgtcga tggcccacgt ggtccgcccg
      961 cccaggagca ttcgggtcgc cgccgccgcg ggcgtcaggg ctgccaggcg gttggtcatg
     1021 acggttgact tgctggctcc gccgtgggag aagtgcaagc caaacgccat gtcgcccctc
     1081 ttcgggccgc tcgtcttccg gaccggcctg tccgccagaa accgcctcgt cctcgctccg
     1141 ttgaccaaca tgcagagcca cgccgacggc tcgctgggcg acgacgagct acggtggctg
     1201 acgtcccggg ccgagggcgg cttcggtctg gtgatgacct gcgcggccca cgtggcccgg
     1261 gacggccagg gctggcgcgg cgagttgggc gtcttcgacg acgcgctgct gcctggcctg
     1321 acgaggctcg ccacggcgct gggggaacac ggggccgcct cgctcgtgca gctgttccac
     1381 ggcggtctgc gggccgatcc ctcgctcacc gggctggtgc cgtggagtgc cagcgagggc
     1441 gacggctgtc gcgcagcaac cgagcaggac ctcacccgag tcatcgccca gttctcggcc
     1501 gcggctcgcc ggtcgcagga ggccggcttc gatggcgtcg aactgcatgg cgcgcacggg
     1561 tatctgttca cgcagttcct gagcgtcacc cagaaccgcc gtctggatca gtggggcggg
     1621 acgctggaga accgcgcgcg tctgctgcgc gaaacggtgc gcgccgttcg ggcggccgtg
     1681 ggcgcgtcgt tcacggtggg cgtgcggctc tcgcccgagg acttcgggaa cgcacgcggc
     1741 ctggacctcg acgagagcgt gcaggttgcg gagtggcttg ccgacgacgg cgtggatttc
     1801 gtgcacctgt cgctctggca ggcactggcc agcacggcca agcgcccgga ggcgaacgcc
     1861 attccgctgt tccggtccgc ggtgccgtcc gatgtacggg tcctggcagc cggcggcatc
     1921 tggactcgcg ccgaagccga ggaggtcctg cgcagaggtg ccgacgccgt ggtgctcggc
     1981 cgctcggcca tcgtcaatgc cgactggcca cggcgcgcag ccgatcctgg ctgggctccg
     2041 cggcggcccc ctgtgacgat cgactcgctg gtggcgagcg ggctgagccg cccgtttgcg
     2101 gagcacatgc gccgcttcaa ggatttcgtg gtcgaggaga gccgatgagg ccagatggcg
     2161 gcggtgtata agatcggcgc catgacgatc agccgccgtt ccgccatcca gcgcgtcgtc
     2221 gctgccgccg ggttcacgat cgggccgctg acgattcccc gtcgcgcgcg gctacaggcg
     2281 ccctccacca ctcctggagc acgcctggtg ctcctcggca cgcaaggcgg gccgaactac
     2341 acagccacgc gcggcgaggc ggccaacgcg gtcgtggtgg acggccagcc gtatctcgtc
     2401 gattgcgggt acggcgcgct cgcggcgctc aagaaggcgg gcgtgaacca ccgccagatc
     2461 gcccgcatct tcctcacgca cctgcacgac gaccacacgg cggatctcgc ggcgctcctc
     2521 aatcgacagt ggaccgacgg ccgtgtcgac cctgttgtcg tggctggccc cttcggcact
     2581 gcgcggctgg tcgaggccgt gatcgcgctt ggcgaggcga acgccgcgat ccgtctcgcc
     2641 gacgaggcgc gcagcgtgaa accggccgag atgttccgcg gccaggatct cgccgcgacg
     2701 gcggcaccgg tcgaggtgta ccgcgacgag cgcgtcaccg tgcgcgcggt cgagaacacg
     2761 cacttcccgg cggagtcgaa ggcgaagatg ccgtaccgct cgctgtcgta tcgcctggac
     2821 gccggcggtc gctcgttcgt cttttccggg gacacggcct actccccagg cctcgtggcg
     2881 ctcgccaggg gcgccgagat tctcgtgtgc gaaggtctgg atatcgccgc gatgcgcaaa
     2941 gcgttcgacg gcatggtggc gcgcgggatg tacgcggaca acccggaagg tgtctggcaa
     3001 cacatcgtcg gcacgcacac gccgctcgag gacgccggac gcatggcggc ggaggccgga
     3061 gtgcggacgc tcgtcctcac gcacctcgtg cccggagcgc tggacccgct gccagacgag
     3121 acgtacctga cccccgcacg aaagacgttc cgcgggcagg tcgtgctcgg cgccgatcag
     3181 ctggtgctgt aggccacgag tgacgtagtc gccgtatcgt ccgcgcaccg ccgccgccgg
     3241 cccacgcgac ctggcggcag ccgtcgtcga tcgtcgctat ttcgcgcggc ccgtataacc
     3301 ctgcgcgatg gtcggaatcg cgatgatctc gtttcgtgcg ctcggtgtcc cccacgtgcc
     3361 gtagaagacg atgccgtaga gcgtcctccg gtccctgccg ccgaagaacg tgccgtgcag
     3421 gccctgcgga cccggaatcg tgccgaggaa tttgccgtcg gccgcaaaca cgtccaccga
     3481 cctgcccgtc gccacgtaca accgtccctg ctgatcgacc gcggatccgt cgccgccctc
     3541 gccgccctgc agttttccga actcgcgctg attggtcagc gagccgtcgg cgttcacgtc
     3601 gaaggcgaag acggtcgcac cattggtcac atacagcgtc ttctcgtcgg ggctcagaat
     3661 gatgccgttg gcggccggca cgtccttccc gtactgggag acgacgcctt tcgggctggc
     3721 gtagaagacg ccgctgtttc tcgcgcccgt gacggagaag tacacgccgc cgctggcgtc
     3781 ggccgtgaga tcgttgatca cgccgccgat gcattcgagg ggctcgccgt tgaacgtgtt
     3841 cgcgagcatc cggcgctgcg gtccgagttg cgcgatgccg ccgccgagtc cgcgttcggc
     3901 gacaaacagc gcgccgttct tgctgcgcga gacggcgccc gcggtattga cgttgccgta
     3961 ggcgaccttc gcgaggttgg tcgccggatc gaactggatg acgttgcccg cgtcgttgtt
     4021 cgcgaacagc atcgtgccgt tctcgccggc gatcgggccg tcggcgttgt tgccttgcca
     4081 cgaccagacg accttccagc tctgtaccgc ctccagcact cccggaatcg acgacgtcgg
     4141 cggcagcacc ggctcgggcg gcgcagcggt ggccgtcgcc gcgtttcccg cactcggaat
     4201 cgcgaacggc tgcggcgggt tcttgcactg cgacagcgtc gtcctgtagt tggcgtgctg
     4261 ccacgcctgc tgactgctgc cgcgatcgaa ggtccccgac gggccttcat tacgcgcgtc
     4321 tttgtatcct ttcgcgcgaa cgtcccaatt gctgttcgtg cagagctcgc actggtcgcc
     4381 gacgaacatc gccgccgctt tcttgtcgtt cttgaactgc cagagcagcc agttcgacgc
     4441 gacgttcgca tattcgccgc cgcccgggtg atccacggta gccgtgtggc ccgcgccgtg
     4501 gcgggcgccg tagaacgccg gcacgttctt gatcagctcg aatgtcgtga gcgacgcggg
     4561 cgtgaggagg tcgcgttcgg cgccgttgac gagcagcacg ggaccgtgga ccttctgcag
     4621 cgcgtcgtcg ttcgagtccg gcctggcgcg ctgcacgccg gagttgaaga cgccgatggt
     4681 cttcacgcga gggtccgagc ccagcgcgat ggagagaaat cctccgcacg actgtcccat
     4741 cacggcgacg tgctcgagat cgactttgcc tttgagcggc gagccgtcgc gcgcgttttc
     4801 tttctccgcc cacgcgatcg ccttgcgcag atcgtcgtcg ttctgctgcc gtgtcgcggc
     4861 gcccggctcc ggcgcgctcc cgatcaccag aaagccgtgc gacgcgatcg tcgtgaacaa
     4921 gcccgagtag cgcgcgctgt tgatggcgca gccgccgttg ccccacgcca tcaccggcag
     4981 cttgtccctg ccggggaatc tgccgagatc ctccggacgg aagacgacgt ggccgggcga
     5041 gccgagcgcg ggctccgaca tgactttgta cgggcccggg atcgctttcg gcgcgccatc
     5101 gagcggcagg gtcgtcctga tcaggtcccc cctgggcccg ggggcctggg cgaacgacac
     5161 acaggcactg aggacgaatg cgacagtaag tgctgatttc atgagcgtag cttagcagac
     5221 ggaagcaggt ccggcgctcg cggcagatcg tccggccagc ggccagtggc ggtagactct
     5281 gctctttaca gaggaggacg tcgatggggg ttcacagtgt ccgctcgcac gggtccattc
     5341 gcgtcgccgc tgcggcgctc gctgtcagcg tgtgcgtcgc cgcccacctg acgggacaga
     5401 ccacggcggt ggactggccg agctacaacc gcacgttcac gtccgagcgc tatgcgccgc
     5461 tcgatcagat cgacaggacc aacgtgtccc gcctgcggca ggtgtgcgtc tacgacctcg
     5521 aggtcgacac caacttccag accggcccga tcgtgatcgg cggcacgctg tacgccacga
     5581 cggaccgcga gatccttgcg ctcgacgcgg cgacctgcca gccgaaatgg cgcgtccgcg
     5641 agccgggtcc ttcggtcggg ctggccgtca atcgcggcgc cgcgtacctc gacggacggc
     5701 tgtttcgagg gacgccggac ggcgacctgg ccgcgtacga cgcgcaaacc ggcaagaggc
     5761 tctggaccac gcgccttgcc gaccgtgaga agggcgagag tgttccgtcg gcgccgatcg
     5821 cgtggaacgg gatggtgttc atcggcacgg cgggaggcga cgtgtacgga gtgaagggtc
     5881 gtatgtacgc gctcgacgcg ggaacgggca aagtgctgtg ggagacctac accgtgccca
     5941 ccgacgcgcc gcagccgggc aacgagaaga tgcaggcgca ggcgaaggtc acgtggggca
     6001 acgagcccgg cgtcccgatc accggcggcg gaacctggtc cagctactcg ctcgacaccc
     6061 agcgtggctt gctgtatctg cccaccggca atccagctcc cgatttcgcg accgacgttc
     6121 gtcccggcga caacctctac acgaactcgg tgctggtgct cgacgccaag acgggcatct
     6181 atcgcaatca ctattcattg gtgcccgctg attttcacga ctgggacctc tccgccgcac
     6241 ccgtgctcgt gaccaccagg ggcggcaagc gcatcgtcgc tggagcgccg aaggacgggt
     6301 tgctgcacgc ctacgatgtg gcgacggaca agaagctgta cgccacgccg gtcacgactc
     6361 gcgagaacga gaacgcgccg ctgtcgacga cacccacgcg tttctgcccc ggcgccgctg
     6421 gagggaccga gtggaacggc ccggcctaca gtcccgacac gaatttcttc tacagcggga
     6481 ccgtggactg gtgcgtgacg gtgacgctcg atccgtcgga gctcgccaag aagccgggtc
     6541 aggcgtggac gggcactcag ctctcggcgc agttcggcgt gcgcgatctc aactgggccg
     6601 gatgggtgac ggccgtcgac gccgacagcg ggcaggtgaa atggcgcgtc cgcgccggtg
     6661 cccccgtggt gtcgggcgtc acgccgacga agggcgggct cgtgttcgcc ggcgacatgg
     6721 acaagcgcgc gtacgcgttc gatgccgaaa cggggacgat cctctggcgc agcgatctgc
     6781 ctggcgctcc tggcggcggc gtcatcacgt acgccgtcgg cggaaagcag tacgtcgtct
     6841 tcgtggcggg cacgcgctcc cgcgtgttcc ccgtctctgc cgcgagcgcc aagatcgtcg
     6901 tcttcgggtt gtgaa
//
LOCUS       DBNGSH010000580         1929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3453818, whole genome shotgun sequence.
ACCESSION   DBNGSH010000580 DBNGSH010000000
VERSION     DBNGSH010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3453818"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="AC16CY_13435"
     CDS             <1..300
                     /locus_tag="AC16CY_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354844.1"
                     /translation="NESTSAFHEFSCWLLSYLEFARGEGRILTRRSGSTPRKNGLVAP
                     HWRPAQLRESPQAARQSRDKPVLHPVADHGAHSRASQNAPFAEATAAAQDGNRER"
     gene            complement(297..1127)
                     /locus_tag="AC16CY_13440"
     CDS             complement(297..1127)
                     /locus_tag="AC16CY_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354845.1"
                     /translation="MNVSLSAYDYVEPGTLSISIHGAKVGGEYTGTLPLDERRHWFAQ
                     ANGRGTVGNVTYDGWCLPWLITPDSASANGYALHLGDPSQCSDGGYEDWFVELRGLVG
                     KDVIGARWALSPYAGVGFRHLSNGTAGIDGFRTQDYLYLPLGITLRSRVASQGVLSFV
                     LEYDYLIHGWQQTRNSALGGGLVPATPTAPAFVLEGLSDVSFAQDSGWALRASAKYQM
                     TRRWFVEPYYVYWHVSDSSVNQVTATFTVNNVTAQKKFGAYEPLNTTHEFGVKTGVRF
                     "
     gene            complement(1231..>1929)
                     /locus_tag="AC16CY_13445"
     CDS             complement(1231..>1929)
                     /locus_tag="AC16CY_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3354846.1"
                     /translation="GWFDGGLWGNRTRFDVHVPMKTTVNVNTAGGGITVSKITGRVGV
                     HTSGGGLQIEAVEGDVNGSTSGGGVRMRDIKGNAVTSTSGGGIEISNVQGTLRASTSG
                     GGIRIDGVTGDLHASTSGGGVTINAAGGRVEAHSSGGPVTARFVAGNNKGGVLSTSGG
                     GVRAEVDPKVALSIDASSSGGGVTADLPITIQGRISNDSLRGDLNGGGATLRLRSSGG
                     GVRLSGLSSSSASR"
BASE COUNT          339 a          662 c          600 g          328 t
ORIGIN      
        1 aacgaaagca catcggcgtt tcatgaattc tcctgctggc tcttatccta tctggagttc
       61 gctcgaggcg aagggcgcat tctgacccgg cgctccggtt cgacgccgcg gaaaaacggg
      121 ctcgtcgccc cgcattggcg acccgcgcag ctccgcgagt ctccacaagc tgcgcggcaa
      181 tcacgcgaca agcccgtttt acacccggtc gctgatcacg gcgctcacag tcgcgcctct
      241 cagaatgcgc ccttcgccga agcaacggcg gcggcgcaag acggaaaccg cgagcgctag
      301 aagcggacgc ccgttttcac gccgaactcg tgggtggtgt tcaagggctc ataggcacca
      361 aactttttct gtgccgtcac gttattgacc gtgaacgtcg cggtgacctg attcaccgac
      421 gagtcgctga cgtgccagta gacatagtac ggctccacaa accagcgcct ggtcatctgg
      481 tacttcgcgc ttgcgcgcag cgcccatcca ctatcctggg cgaacgagac gtcgctgagg
      541 ccctcgagca cgaaggccgg cgcggtcggc gtcgctggaa caagaccacc gcccagtgct
      601 gaattacggg tctgctgcca gccgtggatc aggtaatcgt attcgaggac gaaactcagc
      661 acgccctgag aagccaccct ggagcgcagt gtgatgccga ggggcagata aaggtagtcc
      721 tgcgtccgaa atccgtcgat gccggccgtg ccgttcgaca aatgacggaa ccccacgccc
      781 gcatacggtg acagcgccca tctggccccg atgacgtcct tgccgaccag gccgcgcagt
      841 tccacgaacc agtcttcgta gccgccgtcg ctgcactgcg aaggatcacc gagatgcagt
      901 gcatagccgt tcgcggacgc gctgtctggc gtgatcagcc agggtaagca ccagccatcg
      961 taggtgacgt tcccaaccgt gccccgccca ttggcctggg caaaccagtg ccggcgctcg
     1021 tcgagcggca gcgtgcccgt gtactcgccc cccaccttgg cgccgtggat cgagatgctc
     1081 agcgtaccgg gctcgacata gtcgtaggcg ctcaggctga cattcacttc atgtcccgtc
     1141 ggggtggcca ggctctgggc ggcagaaggg acggggagaa gaaagacgag tatcccggcc
     1201 gttattgact gatgaatcga caaaccgcga tcatcgactc gcggaggacg acgagagacc
     1261 ggacagccgc acgccgccgc cgctcgatcg caggcgcagc gtcgcgccgc ctccattgag
     1321 atcgccgcgc agcgagtcgt tggagatgcg gccctgaatc gtgatcggca ggtcggcggt
     1381 cacgccgccg cccgatgagc tcgcgtcgat cgagagcgcg actttcggat cgacctcggc
     1441 gcgcacgcct ccgccagacg tggacagcac gccacccttg ttgttgcccg ccacgaagcg
     1501 cgccgtgacc ggtccgccgg agctgtgcgc ctcgacgcgg cctccagcag cgttgatggt
     1561 gacgccgccg ccgctcgtgc tggcgtggag gtcgccggtc acgccgtcga tccggatgcc
     1621 tcccccgctc gttgacgcgc gcagcgtgcc ctgtacgttc gagatctcga tgccgccgcc
     1681 ggaggtactg gtgaccgcgt tgcccttgat atcgcgcatg cggacgccgc cgccgctcgt
     1741 ggacccgttg acgtcgcctt cgaccgcctc gatctgcagc ccgcccccag atgtgtgcac
     1801 gcccacgcgg ccggtgatct tcgagacggt gatcccaccg ccggcggtgt tcacgttcac
     1861 cgtcgtcttc atgggcacgt gcacgtcgaa gcgcgtgcga ttgccccaca agccgccgtc
     1921 gaaccagcc
//
LOCUS       DBNGSH010000581         3157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4133576, whole genome shotgun sequence.
ACCESSION   DBNGSH010000581 DBNGSH010000000
VERSION     DBNGSH010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4133576"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /gene="nuoD"
                     /locus_tag="AC16CY_13450"
     CDS             <1..1170
                     /gene="nuoD"
                     /locus_tag="AC16CY_13450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR3354847.1"
                     /translation="RTETMTVNMGPQHPSTHGVLRLVLELDGEVIQSVMPTIGYLHTG
                     IEKTAEQKKWQQVIPLVERMDYLGAQSNSLAYALCVEKMLGLEMPQRVKDIRVLIAEL
                     QRLASHLIWLGTHAMEIGAVSMLFYAIRERELLMNINELIAGFRFFPSYIRIGGLRED
                     LPRGFHDAVNEFLARFPTKMDEFERLLTKNDIFRRRTEGVGVITLADAYQWGLVGPIA
                     RAAGSDYDVRKYFTYTGYEGYDFTVPTATEGDVYARYRVRIAEMRQSIAICEQAISRI
                     TPTGVWACSDTRVVPPPKDKVYTEMEALIQHFLIYSQGFTVPEGDAYVPIEGPRGEHG
                     CYVVSDGSNRPWRVKMRAPSFMAIQALPAVVQGGLIADLVAVIGSTDVVMGDCDR"
     gene            1167..1775
                     /locus_tag="AC16CY_13455"
     CDS             1167..1775
                     /locus_tag="AC16CY_13455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR3354848.1"
                     /translation="MSFHPPMEYAPARFHKSERKLAEAGEAFTYTPENQAKFDENVGR
                     YPSDQRKSAILFALYLAQQQQGYITGAAMRFVAGQIGCTPAEVEDVVSYYTMFYTRPV
                     GKYVLNVCRTLSCALLGAERVTEELCGKLGVRPGETTADGAFTVIEVECLGACDRAPV
                     VMVNDDGWHERLSPEQAAAFVDDIRTRGRAAFTGCHLHVEKK"
     gene            1881..>3157
                     /gene="nuoF"
                     /locus_tag="AC16CY_13460"
     CDS             1881..>3157
                     /gene="nuoF"
                     /locus_tag="AC16CY_13460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011426948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR3354849.1"
                     /translation="MAFESVLTNHVREPNSFTLDFYVKRARGYEGLRKALTLKPGDII
                     EQVKASGLRGRGGAGFPTGLKWQFVLKDTPKPKYIACNADESEPGTFKDHVLMERNPH
                     LLLEGCAIGCYAIGAKVAYIYIRGEFYHVQETLERAIEEAYRAGFLGRNILGSGFDCD
                     IYVHRGAGAYEAGEETALIESLEGKRAQPRIKPPFPAVAGLYQCPTAVNNVETLCNVP
                     LIVLNGPEWFAGLGPEKNGGPKLFCISGHVKRPGTYEASMHTTLRELIYGDQYAQGMA
                     GGRKFKAIIPGGSSVPILLEPQLDTPASFDHIQKAGSLLGSAGLIVMDDSVCMVWLAM
                     NLLHFYRHESCGKCTPCREGGDWLYKLLQRVEGGEGRMKDIDLLYSVASNIVGKTLCA
                     FGDAAATPVLTTLNLFRHEFEAHIREGRCTLPA"
BASE COUNT          568 a         1024 c         1063 g          502 t
ORIGIN      
        1 cgcacggaaa cgatgacggt caacatgggg ccccagcacc ccagcacgca tggcgtgctg
       61 cgtctcgtgc tggagctcga tggggaagtc atccagtccg tgatgccgac gatcggctac
      121 ctgcacaccg gcatcgagaa gaccgccgag cagaagaagt ggcagcaggt catcccgctc
      181 gtcgagcgga tggactacct gggcgcgcag tcgaacagcc tcgcctacgc tctctgcgtc
      241 gagaagatgc tggggctcga gatgccgcag cgcgtgaaag acatccgcgt gctcatcgcg
      301 gagctgcagc ggctggccag ccacctgatc tggctcggta cccacgcgat ggaaatcggc
      361 gccgtgtcga tgctcttcta cgccatacgc gagcgcgagc tcctgatgaa catcaacgag
      421 ctcatcgccg gattccgctt ctttccgagc tacatccgca tcggcgggct tcgcgaggat
      481 ctgccgcgcg gcttccacga cgccgtgaac gagttcctcg cgcgctttcc cacgaagatg
      541 gacgagttcg agcgtttgct gacgaagaac gacatcttcc gccggcgcac ggaaggcgtg
      601 ggcgtgatca cgctcgccga cgcctaccag tggggcctcg tcgggcccat cgcccgggcg
      661 gccgggtccg actacgacgt ccggaagtac ttcacctaca ccggctacga ggggtacgac
      721 ttcaccgtgc cgactgcgac cgaaggcgac gtctacgcgc ggtatcgcgt gcgcatcgcc
      781 gagatgcggc agagcatcgc gatctgcgag caggcgatca gccggatcac gccgaccggc
      841 gtgtgggcgt gcagcgacac ccgcgtcgtg ccgccgccga aggacaaggt gtacaccgag
      901 atggaggcgc tgattcagca cttcctgatc tactcgcagg gcttcaccgt gccggaggga
      961 gacgcctacg taccgatcga ggggccacgc ggcgagcacg ggtgttacgt cgtgtccgac
     1021 ggcagcaacc gcccctggcg cgtgaagatg cgggcgccgt ccttcatggc gattcaggcg
     1081 ctgcccgccg tggtgcaggg cgggctgatc gcggacctgg tggccgtcat cggcagcacc
     1141 gacgtcgtca tgggagactg cgaccggtga gctttcatcc cccgatggag tacgcgccgg
     1201 cccggttcca caagtcggaa cggaagctgg ccgaggcggg cgaggcgttc acgtacacgc
     1261 ctgagaacca ggcgaagttc gacgagaacg tcgggcgcta tccatccgat cagcgcaagt
     1321 cggccattct ctttgccctc tacctggcgc agcagcagca ggggtacatc accggcgccg
     1381 cgatgcgttt cgttgccggg cagatcggct gcacgccggc ggaggtcgag gacgtcgtgt
     1441 cgtactacac gatgttctac accaggcctg tcgggaagta cgtgctcaac gtctgccgga
     1501 cgctctcgtg cgcgctgctc ggcgccgagc gcgtcaccga ggaactgtgc ggcaagctcg
     1561 gcgtccgtcc cggcgagacg acggccgacg gcgcgttcac ggtgatcgaa gtcgaatgcc
     1621 tcggcgcctg cgaccgggcg ccggtggtga tggtgaacga cgacgggtgg cacgagcggc
     1681 tgtcgccgga acaggcggcc gccttcgtgg acgacatccg cacgcgcggc cgggccgcgt
     1741 ttacgggctg tcatctgcac gtggagaaga aatgaaccac gaagctcacg aagatccaga
     1801 aggacacgaa gtgacccttc ctggcctctg gtgttcttcg tggccttcgc gatcttcgtg
     1861 ttcttcgtgg ttaagagatc atggcgtttg aatcagttct cacgaaccac gtgcgcgagc
     1921 cgaactcgtt cacgctcgac ttctacgtga agcgcgcgcg cggctacgag gggctgcgga
     1981 aggcgctcac cctcaagccg ggcgacatca tcgagcaggt gaaggcgtcg gggctccgcg
     2041 ggcgcggcgg cgccggcttc ccgaccggcc tcaagtggca gttcgtcctg aaggacacgc
     2101 cgaagccgaa gtacatcgcc tgcaacgccg acgagagcga gcccggcacg ttcaaggacc
     2161 acgtgctcat ggagcgcaac ccgcacctgc ttctcgaagg gtgcgcgatc ggctgctacg
     2221 ccatcggggc caaggtcgcc tacatctaca ttcgcggcga gttctaccac gtgcaggaga
     2281 cgctcgagcg cgcgatcgag gaggcgtacc gggccggctt cctgggcagg aacattctcg
     2341 gcagcggctt cgactgcgac atctacgtcc atcgcggcgc cggagcgtac gaagccggcg
     2401 aagagacggc gctcatcgag tcgctcgaag gcaagcgggc gcagccgcgc atcaagcctc
     2461 cgttccccgc cgtggcgggg ctctaccagt gcccgaccgc ggtcaacaac gtcgagacgc
     2521 tgtgcaacgt cccgctgatc gtcctcaacg gtccggagtg gttcgccggc ctcggtcccg
     2581 agaagaacgg cggaccgaag ctgttctgca tcagcggcca cgtgaagcgg cccggcacct
     2641 atgaagcctc gatgcacacg acgctgcgcg agctgatcta cggcgaccag tacgcacagg
     2701 gcatggcggg cggacggaag ttcaaggcca tcatccccgg cggatcgtcg gtgccaatcc
     2761 tgctcgagcc gcagctcgac accccggcca gcttcgacca catccagaag gccggatcgc
     2821 tgctcgggtc cgcgggcctc atcgtgatgg acgacagcgt ctgcatggtg tggctcgcga
     2881 tgaacctgtt gcacttctac cggcacgagt cgtgcgggaa gtgcacgccg tgccgcgagg
     2941 gaggcgactg gctgtacaag ctgctgcagc gcgtggaggg cggcgagggt cgaatgaagg
     3001 acatcgacct gctctacagc gtggccagca acatcgtggg caagacgctc tgcgccttcg
     3061 gcgacgccgc ggcgacgccg gtgctgacga cgctgaacct gttcaggcac gagttcgagg
     3121 cgcacatccg cgaagggcgg tgcacgctgc cggccga
//
LOCUS       DBNGSH010000582         4364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_397607, whole genome shotgun sequence.
ACCESSION   DBNGSH010000582 DBNGSH010000000
VERSION     DBNGSH010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_397607"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="AC16CY_13465"
     CDS             <1..1125
                     /locus_tag="AC16CY_13465"
                     /EC_number="1.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016670 - oxidoreductase activity, acting
                     on a sulfur group of donors, oxygen as acceptor [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3354850.1"
                     /translation="WMWAVLFAGTVVAVLLVNARKTATPSASPAAGAMPAPSTFLPTV
                     VNPATPPGPAPEGMVWIPGGEFSMGAADPTGSDNNHVGMQATHDSRPIHRTYVDGFWM
                     DATEVTNAQFAAFVKATGYVTVAEKTPKAEDFPGAPPENLVAGSVTFSPPDHAVPLND
                     HFRWWAYVKGASWRRPLGPSSPIAGREKYPVVHIAYEDAVAYSTWAGKRLPTEAEWEF
                     AARGGLSGQVYPWGQQFRPEGAWMANSHQGHFPDHDDGGDHFTGVAPVAQFPSNAYGL
                     YDVAGNVWEWVSDWYRPDYYAQLAAAGGVARNPQGPASPFDPDEPGVQKRVHRGGSFL
                     CTDQYCSRYMVGTRGKGDISTGTNHLGFRAVMSPKTSSQG"
     gene            1180..1698
                     /locus_tag="AC16CY_13470"
     CDS             1180..1698
                     /locus_tag="AC16CY_13470"
                     /inference="COORDINATES: protein motif:HMM:NF022526.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HIR3354851.1"
                     /translation="MTRHHLGIVCVAFLFAGSASSDAAAQSTTQKAPAPTAQATAAAK
                     ASPQLVSALSKELGATPEQAAGAAGSLFSVAKSRLKPDEFSQVSKAVPGMDALLKAAP
                     STSGGGALGGVSGALSSLGGSSSGLAGAMSAFSKLGLKPEMVAKAIPILTSFVSKSGG
                     ANVGSLLAGVLK"
     gene            1739..2308
                     /locus_tag="AC16CY_13475"
     CDS             1739..2308
                     /locus_tag="AC16CY_13475"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3354852.1"
                     /translation="MRKLACVMAAAAVVACVACGDSQDDSISTSVGAKLTADQKAGTS
                     QVKVETKDQVVTLSGTVDTPAAKEQAVRAARETEGVREVVDNIQVSGAESAEAAAQTG
                     AGIGDSAAKAATATGDAAKATGDVVVDSAKATGNVIVEGAKATGEAVAVGAKATGEAT
                     VKGAEATASGAKKLGSGILGAVTPDKEKK"
     gene            complement(2321..>4364)
                     /locus_tag="AC16CY_13480"
     CDS             complement(2321..>4364)
                     /locus_tag="AC16CY_13480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354853.1"
                     /translation="GRTFLPGEDQPGSDRVVLLSHVLWERRFGSDPAMLGRDVVLNGE
                     AYTVIGVLPKGSPFDRTAAQIWKPLAFQPSHMTRDYRWLGATGKLKPGVTLRNARAQM
                     DVIGQRIANAYPDSNRGWGVAVDRLADAVVGPQLRTAVTVLFAATGLVLLIGCANLAN
                     LALARGISRERDMAVRAALGASRWRLVWQLLIENILISVCGGLAGVGVGYAMLNWIQS
                     LIPPETLPPAMDVRMDTRVLLFALIVAVATGLLFGIWPAARTTTPSPVGALKEGGHTT
                     AGRRGRRLLDVVVVAEIGLAFVLLVASGLLLRSVLELLDIDPGFDATNVLTAGLPITQ
                     EQHADPVELNAYLASIRAAVEAVPGVRATAMTSALPLLGGGYGVRYAIADRDFIDRAD
                     RRRAFFKIVSPSYFGALGITLVAGRALSDNDTAGAPPVALINETLARREFPDGDPIGR
                     RILVQEIVPGRTELGREIAWEIVGVIAREKVTGLGDEISAGMYVSNQQSPTYDLNLIV
                     QAGMPPQSLQRAVRSAVESVNRDQALSDVRTLEQIVDQSLLGNRVLATLLAGFAFVAL
                     LLAAGGLSGVMSYTAAQRTHEMGIRAALGASAGNLRTLVFKGGMRLTLIGLAIGLAGT
                     LAATRVIASMLYGVGANDPLTIAVAVAVLSVVAGLACVVPAWRTTKGDPMQALRYR"
BASE COUNT          780 a         1451 c         1485 g          648 t
ORIGIN      
        1 tggatgtggg ccgtgctgtt cgccggcacc gtcgtcgccg tgcttctcgt caacgcgagg
       61 aaaacagcga ctccgtcggc gtcacccgcg gcaggcgcga tgccggcgcc gtcaacgttc
      121 ctgccaaccg tagtgaaccc tgcaacccca cccggtccgg cgccggaagg catggtgtgg
      181 attccaggcg gcgagttctc gatgggcgcg gccgatccga ccggctcgga caacaaccat
      241 gtcggcatgc aggccaccca cgactccagg ccgatccatc gcacctacgt ggacggcttc
      301 tggatggacg cgaccgaagt caccaacgca cagtttgcgg cgttcgtgaa ggcgaccggg
      361 tacgtgacgg tcgcggaaaa gacgccgaag gcggaggact ttccaggcgc gcctccggag
      421 aatctcgtgg ccggctccgt gacgttttca ccgcccgatc acgccgtgcc gctcaacgat
      481 cactttcgat ggtgggccta cgtcaagggc gcaagctggc gtcgtcccct cggtccgtcg
      541 agtccgatcg ccgggcggga gaagtatccc gttgtgcaca tcgcctacga ggacgccgtg
      601 gcatattcga cgtgggcggg caaacgcctg cccacggagg cggagtggga gttcgcggcg
      661 cgcggcggtc tgtcgggtca ggtgtatccg tggggacaac agttcagacc cgagggcgcg
      721 tggatggcca acagccatca aggccacttc cccgatcacg acgacggcgg cgatcacttc
      781 accggtgtgg cgcccgtggc gcagtttcca tccaacgcgt acggcctcta cgacgtggcc
      841 ggcaacgtgt gggagtgggt cagcgactgg tatcgcccgg actactacgc ccaactcgcg
      901 gcggcgggag gcgtcgcgcg gaatccgcag ggaccggcct cgcccttcga ccccgatgag
      961 ccgggcgtgc agaagcgcgt gcaccgcggc ggatcgtttc tctgcacgga ccaatactgc
     1021 tcgcgctaca tggtcgggac gcgcggcaag ggcgacatca gcactggcac gaaccacctc
     1081 gggtttcgcg ccgtgatgtc gccgaagaca tcttctcagg gctgatcgtt gacgccaaag
     1141 taatgccgct gcgggctgca gcggcgcagg agggcaccca tgactcggca tcatctcgga
     1201 atcgtgtgcg tcgcattcct cttcgctggg agcgcctcga gcgacgcggc ggcacaaagc
     1261 accacgcaga aggcgccggc ccccaccgcg caggcgacgg ctgccgccaa ggccagccct
     1321 cagctggtca gcgcgctctc gaaggaactg ggcgcgactc ccgaacaggc cgcgggcgcg
     1381 gctgggtcgc tcttcagcgt ggcgaagtca cggctcaagc cagatgagtt ctcacaggtc
     1441 tcgaaagccg tgccaggcat ggatgcgctg ctcaaggccg cgccttcgac cagcggcggc
     1501 ggcgccctgg gcggcgtatc tggcgcgttg tcctcgctgg gcgggtcatc gagcggtctt
     1561 gcaggcgcga tgagcgcctt ctcgaagctc ggcctcaagc cggagatggt cgccaaagcg
     1621 atcccgatcc tgacatcgtt cgtcagcaag tccggcggcg ccaacgtcgg cagcctgctc
     1681 gccggcgtgc tgaaatagcg tgagagtctc cccccgccgg agttcaatgg aggatccaat
     1741 gagaaaactg gcttgcgtca tggctgcggc ggcggtggtc gcctgtgtgg cgtgcggcga
     1801 ttcccaggat gacagcatct cgacgagcgt gggcgcgaag ctcacggccg atcagaaggc
     1861 gggcacgtcg caggtgaagg tcgagacgaa ggatcaggtc gtcacgctca gcggcacggt
     1921 tgacacgcct gccgccaagg aacaggccgt ccgcgcggcg cgcgaaacgg aaggcgttcg
     1981 cgaggtcgtg gacaacatcc aggtctcggg cgcagaaagt gcggaggcgg ccgctcagac
     2041 cggcgcgggg atcggggaca gcgcggcgaa ggctgcgacc gcaacgggcg acgcggcaaa
     2101 agccaccggc gacgtggtcg tcgactcggc gaaggcgacc ggcaacgtca tcgtggaagg
     2161 ggcgaaggca accggcgagg ccgttgcggt aggagccaag gccacgggtg aggctaccgt
     2221 caagggtgca gaggccacag cctcgggcgc gaagaagctg ggcagcggca tcctcggcgc
     2281 cgtgacgccg gacaaggaga agaagtaagg cgcactcgct tcatcgatag cgcagcgcct
     2341 gcattggatc gcctttcgtg gtccgccagg ctggcacgac gcacgccagc ccggcgacca
     2401 ccgagagcac agcaaccgca acagcgatcg tgagcgggtc gttggcgccg acgccgtaga
     2461 gcatcgacgc gatgacgcga gtcgcggcaa gcgttccggc aagcccgatc gcgagcccga
     2521 tcagcgtgag ccgcatgccg cctttgaata ccagcgtccg caaattgccc gcgctggctc
     2581 cgagcgccgc gcgaatgccc atctcgtgcg tccgctgcgc cgccgtgtag gacatcacac
     2641 cggacaagcc cccggccgcc agcagcagcg cgacgaaggc gaacccggcg aggagcgtgg
     2701 ccaggacgcg gttgcccagc agggattgat ccacgatctg ttcgagcgtg cggacgtcgc
     2761 tgagcgcctg gtcacggttg acgctctcca cggctgaccg aacggctctc tgcagcgact
     2821 gcggaggcat gccggcctgc acgatcaggt tgaggtcgta ggtcgggctc tgctggttcg
     2881 agacgtacat gccggcgctg atctcgtcgc cgaggccggt gaccttctca cgcgcaatga
     2941 cgccgacgat ctcccaggcg atctcccggc ccagctcggt cctgcccggt acgatttcct
     3001 gcacgaggat gcggcggccg atcgggtccc catcgggaaa ctcccgcctg gccagcgttt
     3061 cgttgatgag cgccacgggt ggtgcgcccg cggtgtcgtt gtcgctcaac gcgcgtcccg
     3121 cgaccagcgt gatgcccagc gcgccgaagt aggacgggct gacgatcttg aagaacgcgc
     3181 ggcggcgatc ggcccggtcg atgaagtcgc gatcggcaat cgcgtagcgg acaccgtagc
     3241 ccccacccag cagcggcaac gccgaggtca tggccgtcgc ccgaacgccg ggcacggcct
     3301 ccacggcggc acggatcgag gcgagatagg cgttcagctc gaccgggtcg gcgtgctgtt
     3361 cctgcgtgat cggcaggcct gccgtgagga cattcgtcgc atcgaacccc ggatcgatgt
     3421 cgagcagctc cagaacactt cgcaacaata gtcccgacgc caccagcagc acgaacgcca
     3481 gcccgatttc agccacgacg acaacgtcga gtaggcgccg gccccggcgg cccgcggtcg
     3541 tgtgtccgcc ttccttcagc gcgcccaccg ggctcggggt cgtggtccgc gctgccggcc
     3601 aaatgccgaa caacagtccc gtcgcgacgg caacgatcag cgcgaacagc aggacgcgcg
     3661 tatccattcg gacgtccatt gcgggaggca gcgtctcggg aggaatcaac gattgaatcc
     3721 agttcagcat cgcgtaccca acgccgactc ctgcgaggcc accgcacacg gagatcagga
     3781 tgttctcgat cagcaactgc cacaccaacc tccaccggct tgcgccaagc gcggcgcgca
     3841 cggccatgtc gcgctccctg gagatacctc gcgcaagcgc gagatttgcc aggttggcgc
     3901 aaccgatcag cagcaccaac ccggtcgcgg cgaagaggac cgtgacggcc gtgcgcagct
     3961 gcgggccgac caccgcgtcg gcgaggcggt caacggcaac gccccaccct ctgttcgaat
     4021 cgggataggc gttggcaatg cgctgcccga tgacgtccat ctgggccctt gcgttcctga
     4081 gcgtcacgcc cggcttgagc tttccggtcg caccgagcca gcggtagtcg cgcgtcatgt
     4141 gtgacggctg gaaggcgaga ggcttccaaa tctgggctgc ggtgcgatcg aaggggctgc
     4201 ctttcggaag aacgccgatc acggtatagg cctcgccgtt caacacgacg tccctgccca
     4261 gcatcgcggg atcggagccg aagcggcgtt cccagagcac atggctcagc agcacgacac
     4321 gatcgctgcc gggctggtcc tcgcctggaa gaaacgtgcg gccc
//
LOCUS       DBNGSH010000583         2567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1416018, whole genome shotgun sequence.
ACCESSION   DBNGSH010000583 DBNGSH010000000
VERSION     DBNGSH010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1416018"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="AC16CY_13485"
     CDS             <1..739
                     /locus_tag="AC16CY_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3354854.1"
                     /translation="EHMKFWVDALGGVKTTFGANKLEIVKFPNALLFMRAQAPKGGGS
                     KGSTVDHIAFSVPNLRQVVDRIKAGGFKMVTAAEAPPNVPVKDDIGMVQGGGPVSGIA
                     YAMAPDDVKVEIVEMKGQSAPVVSHHVHFFGAQKEMHAWYRQTFGATDVPSANPAAFI
                     SAGLPGLTMNFAPSQAPVAGTQGRALDHIGFEVKNLEAFTKKLESQGIKLTVAYRQVP
                     ALGIAIAFVNDPWGTSIELTEGLDKVQ"
     gene            806..1147
                     /locus_tag="AC16CY_13490"
     CDS             806..1147
                     /locus_tag="AC16CY_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3354855.1"
                     /translation="MPPRADLLPGTLDVLVLKAVSLGPLHGYGVLLRIEQMTGGALLV
                     EQGALYPALFRLEQQGLLDTEWGVSDNNRKAKFYRLTATGRRRLKDDMASWNRLSDAM
                     TAALSTRVGEV"
     gene            1152..>2567
                     /locus_tag="AC16CY_13495"
     CDS             1152..>2567
                     /locus_tag="AC16CY_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354856.1"
                     /translation="MWTRIRLWIASLLRRRRFDEDLTEELEFHLRARAEHWEARGLSP
                     SEARRRARLEFGSVHSVSADVRDVRRGAWVEHIARDVRYGLRSLRRYPAFSAMAVLSL
                     AIGIGANAVIFGAVHAVLSRDSTLANPETLVNIYETEGGQGFNLPSYRNIEDLRKSTT
                     EVFSGIAVSTFFPAPIDGGGTVRVVMGEVVSGGAFALFGVEPLLGRAILPADDVALGG
                     HPVVMLSYGYWQRAFGADPNVVGRTLRIGGRDYEIIGVAPAQYRGAFPLLTPAFYVPM
                     AMANELMGVDMLGQRDFHSFFVKARLAPGATRVQAEHTASLVAESLTRARPEGWVPGE
                     RFSLVPTADVQIYPGVDPLLRAASLLLVVVVGLVLLLACTNLASFLLARALDRGRETA
                     VRRALGATRGALVRQQLVESALLGLGGAAVGLVLALVLLRALVSIDLPLPYGLRLDLN
                     FGIDSTVLFGWRVLALTAGAGV"
BASE COUNT          394 a          838 c          900 g          435 t
ORIGIN      
        1 cgagcacatg aagttctggg tggacgccct tggcggcgtc aagacgacgt tcggcgcgaa
       61 caagctcgag atcgtcaagt tcccgaatgc gctgctgttc atgcgcgcgc aggcgcccaa
      121 gggcggcggc agcaagggga gcacggtgga ccacatcgcg ttctcagtgc ccaacttgcg
      181 ccaggtcgtc gatcgcatca aggccggcgg cttcaagatg gtgaccgcgg ccgaggcgcc
      241 tcccaacgtt ccggtcaagg acgacatcgg gatggttcag ggcggcgggc cggtctccgg
      301 catcgcctac gcaatggcgc ccgatgacgt gaaggtcgag atcgtggaga tgaaggggca
      361 gtcggcgccc gtcgtgtcgc atcacgtcca cttcttcggg gcgcagaagg agatgcacgc
      421 ctggtacagg cagacgttcg gcgccaccga cgtcccgtcg gcgaatccgg cggcgttcat
      481 cagcgccggg ctgcccggcc tcacgatgaa cttcgcgccg tcgcaggctc ccgtggcggg
      541 cacgcagggg cgcgcgctcg atcacatcgg gttcgaggtg aagaatctcg aggcgttcac
      601 gaagaagctg gaatcacagg gcatcaagct caccgtcgcg taccggcagg tgccggcgct
      661 cggcatcgcg atcgcgttcg tcaacgatcc ctggggcacc tccatcgagc tgaccgaagg
      721 attagacaag gttcagtaga ggctcacccc ttgacagctt aggagagcgt caggtagtct
      781 cgcctaagac acttaggaga ctgccatgcc gccccgcgca gacctgcttc ccggcacgct
      841 cgacgtgctc gtcctcaagg ccgtgtccct gggcccgctg cacggctacg gcgtcctgct
      901 ccgcatcgag cagatgacgg gcggcgccct gctggtcgag cagggcgccc tgtatccggc
      961 gctcttccgc ctggagcagc aggggctgct cgataccgaa tggggcgtct ccgacaacaa
     1021 ccggaaggcc aagttctacc ggctgaccgc aacaggccgg cgccggctga aggacgacat
     1081 ggcgagctgg aaccggctgt ctgacgccat gacggccgcg ctgagcacac gcgttggaga
     1141 ggtctgacgc catgtggacg cgcatccgcc tgtggatcgc aagccttctg cgccgccgcc
     1201 ggtttgacga ggatctgacg gaggagctcg agttccatct gcgtgcgcgc gcggagcact
     1261 gggaggcgcg cgggctgtcg ccgtcggagg cgcggcgccg ggcgcggctg gagttcggca
     1321 gcgtccactc cgtcagcgcc gacgtccggg acgtacgacg cggcgcctgg gtggagcaca
     1381 tcgcccggga cgtccgctac ggcctgcgct cgctgcgccg gtatccagcc ttctcagcga
     1441 tggccgtgct gtcgctcgca atcggcatcg gcgcgaacgc cgtcatcttt ggcgcggttc
     1501 acgcggtgct ctcccgcgat tccacgctcg cgaaccccga gacgctcgtc aacatctacg
     1561 aaaccgaagg cgggcagggg ttcaatctgc cgtcgtaccg gaacatcgag gacctgcgca
     1621 agagcacgac cgaggtcttc agcgggatcg cggtgtcaac gttcttcccg gcgcccatcg
     1681 atggaggcgg gacggtacgg gtcgtcatgg gcgaggtggt gtcgggaggc gcgtttgcgc
     1741 tcttcggcgt cgagccgctg ctcggcaggg ccattcttcc cgcggacgac gtcgcgctgg
     1801 gcggacatcc ggtggtgatg ctcagctacg ggtactggca acgcgccttc ggcgccgacc
     1861 cgaacgtcgt cggtcgaacg ctgcgaatcg gagggcgcga ctacgagatc atcggcgtcg
     1921 caccggccca gtaccggggc gcgttcccgc tcctgacgcc ggccttctac gtcccgatgg
     1981 cgatggcgaa cgagctgatg ggcgtggaca tgctcggtca gcgggatttc cacagcttct
     2041 tcgtcaaggc gagactcgca ccaggcgcga cgcgagtaca ggcggaacac acggcctccc
     2101 tcgtcgccga aagcctgacg cgcgcacggc cggaggggtg ggtcccgggc gagcgcttct
     2161 cgctcgtacc gaccgccgac gtgcagatct accccggcgt ggacccgctg ctgcgtgcgg
     2221 cgtcattgct gcttgtcgtg gtggtggggc tggtgctgct tctagcctgc acgaaccttg
     2281 cgagctttct gctggcgcgc gccctcgatc gaggaagaga gacggcggtg cggcgcgccc
     2341 tcggcgcgac caggggcgcg cttgtccgcc agcagctcgt cgagagcgcg ttgctcgggc
     2401 tcggcggcgc ggccgttggc ctcgttctgg cgctcgtgct cctccgtgct ctggtgtcga
     2461 tcgacctccc gctgccgtat ggtttgaggc tcgatctgaa cttcggcatc gactcgacgg
     2521 tgctcttcgg ttggcgggtc ctggcactga ctgcgggcgc gggcgtg
//
LOCUS       DBNGSH010000584         5516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2265741, whole genome shotgun sequence.
ACCESSION   DBNGSH010000584 DBNGSH010000000
VERSION     DBNGSH010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2265741"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="AC16CY_13500"
     CDS             complement(<1..379)
                     /locus_tag="AC16CY_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354857.1"
                     /translation="MPRIRAALLAAAALGIPSSLPAQTTLTLDQAIANAVSGNRALLA
                     DRSRVHEAELGVQEAKSSYFPRLTVGESWQRSNQPVFVFSSLLSSREFAASNFAIDAL
                     NHPDPLPFFRTSVGVEQLLYDGGR"
     gene            471..1082
                     /locus_tag="AC16CY_13505"
     CDS             471..1082
                     /locus_tag="AC16CY_13505"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3354858.1"
                     /translation="MQDPGPVTRNAGPDDVREGYASLVRAAASGDRGAMEQLLVRAQE
                     VAYRVSLLVCGHTEDAEDVMQDALLKTYQYVGRIEDPGAFRTWLYRIVRNACLMKRRR
                     RVGEPVRHVSIEQGGEDEARPVDVEDRSRRADEQLIDVWIGDRLRRALDKLSPSHRAI
                     VVLREIEGLSTKEVAAITQLSEANVKTRLHRARAILRQQLEDL"
     gene            1112..1360
                     /locus_tag="AC16CY_13510"
     CDS             1112..1360
                     /locus_tag="AC16CY_13510"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3354859.1"
                     /translation="MLAQIEAYVDGELGALQCSAIEQHCEQCERCAAIVHGLRRTIGL
                     CQEIGRAPLPPSVSQLARDQVRRLLESSHDSPLGRKAT"
     gene            1431..2147
                     /locus_tag="AC16CY_13515"
     CDS             1431..2147
                     /locus_tag="AC16CY_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR3354860.1"
                     /translation="MECPRCGSAMQQETFDGRLGRSVAIDICHACQSFWFDTRESVAL
                     TPGATLALFRIIGEKLTRPRHSDAELAKCPRCKGRLRRTRDMQRGTKFEYLNCPNGHG
                     RLISFFDFLREKDFIRPLSTRQIAELRENVGAVNCTNCGGAVDLTKGTACPHCGSPLS
                     MLDIHQAEQLVSQLQKAEDRANQPVDPSLPLQLLRVRRETERAFAGLPHDDVWVEDVL
                     KTDLVGAGLSAVARWLKRHS"
     gene            2213..3286
                     /locus_tag="AC16CY_13520"
     CDS             2213..3286
                     /locus_tag="AC16CY_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3354861.1"
                     /translation="MGIMDFIKGELLEIIEWTDDSRDTLSWRFPDDDKAIKNGAQLIV
                     RESQVAQFVYLGEFGDTFGPGKHRLTTDNIPVLTKIKSWPYGFNSPFKADVYYVTTRL
                     FTGNKWGTSNPVMVRDNDFGMVRVRAFGTYDFKVIDTKTFLKEVAGSDHNFRLDEFAD
                     TMRSRIVSIFTDAIATAKIPVLDVASRYTELGEALLPLINPAIKSKYGLELGSFIVEN
                     VSVPPEVEEAIDKRASMAAVGNLNDFVKYQMGKGFEGGGGTGPAGTAAELAVGFGLAQ
                     QMMKEGFMGGTATPTVAAGVPAAQPGGAALPELLSPAEAAQALGVSEADVMSVLDSGD
                     LKGRKIGSTWRISRAALTSYMSQ"
     gene            3283..4389
                     /locus_tag="AC16CY_13525"
     CDS             3283..4389
                     /locus_tag="AC16CY_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR3354862.1"
                     /translation="MSATTEIAALDKHECPACGGQAEWNPGKQKLICPFCGTESPYVI
                     DREQGKVVELDLVKALRELPADERGWQTDRRSVQCQSCRAVMVFDPSRVGQNCEFCGS
                     PALVDYQEIKSPITPQGVLPFRIDTGRVRDDIRRWWKSKWFAPGRLARTALVDTIKSL
                     YVPYWTFDAQVHCPWTADAGYYYYVNVPGVDAKGRPVVRRERRVRWEPAAGAVDTFFD
                     DEPVPGTRGLPLDLLKQVEPFPTKELVPYDTAFLSGHVVEHYQVVLLDAAQRSVEQMQ
                     DRLMALCAQQVPGDTHRNLRIDPAYSGRTFKHVLVPVWLLSYDYASKAYQVIVNGYTG
                     KIAGRHPYSFWKIAFAVLLAAIAALIVVWLNAQQ"
     gene            4559..5404
                     /locus_tag="AC16CY_13530"
     CDS             4559..5404
                     /locus_tag="AC16CY_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354863.1"
                     /translation="MRNLIGVAAVVAIALASAPLSAQWPSHPTPGVPRLESGAVNMDA
                     PVPRTADGKPDLSGNWVRFATPGGRRGAGPAAPAPPPPPGTPPVATFGEVGAGFPEGL
                     PFTPWAAELKKQRMSVQQKDNPDALCLPMGFMQFHTHGQPRKVIQTPGLVTIIYEANY
                     GLRYIFTDGRTLPPQGDPQPWWYGYSVGRWEGDTLVVETNNLRDDGWLDVRGSPYTDQ
                     AKITERFRRVSFGKLEIDITIDDPKAYTKPFTVRVNQQISVDNELIEFICNENQQFRK
                     LIKID"
BASE COUNT         1011 a         1773 c         1840 g          892 t
ORIGIN      
        1 cccgtccgcc gtcgtacagc agctgctcca cgcccacgct ggttctgaag aaggggagtg
       61 gatcggggtg attgagggcg tcgatcgcga agttggaggc cgcaaattcg cgcgacgaca
      121 gaagcgaact gaacacgaag accggctggt tgcttcgctg ccaggactca cccaccgtca
      181 ggcgagggaa atagctcgac ttggcttcct gaactccgag ctcggcctcg tgaacacgcg
      241 agcggtcggc cagtaacgct cgattgcctg acaccgcgtt ggcaatggcc tggtcgagtg
      301 tcagtgtggt ttgggccggc agcgagctcg ggattcccag agcggccgca gccagcaggg
      361 ctgcccgtat acgaggcatg cacgatggat gcaggaaatc ggaattggtg acgagggtag
      421 aatttctcgc gttgccgcct gtgaccgatt ccgagaacct gcatccttcg gtgcaagacc
      481 ctgggcccgt cacccggaat gccgggcccg atgacgtccg ggagggctac gccagcctgg
      541 tccgggcggc cgcgtccggc gatcgcggcg cgatggaaca gctgctggtt cgggcgcagg
      601 aggtggccta ccgtgtcagc ctgctggtct gcggacacac ggaggacgcc gaggacgtca
      661 tgcaggacgc cctgctgaag acgtaccaat acgtcggccg catcgaggat cccggcgcat
      721 tccgcacgtg gctgtatcgg atcgtgcgca acgcctgcct gatgaagcgg cgccggcgag
      781 tgggcgagcc ggtacggcac gtgtcgatcg aacagggtgg agaagacgag gcgcggcccg
      841 tggacgtcga ggaccgatca cgacgggccg acgagcagct catcgatgtc tggattggag
      901 atcgtcttcg acgggcgctg gacaagctgt cgccttcgca tcgcgcgatt gtcgttctcc
      961 gcgagatcga gggattgtcg acgaaggaag tggccgcaat cacgcaactc tcggaggcca
     1021 acgtcaagac gcgcctgcat cgcgctcgag cgatcctccg gcagcaattg gaggacctat
     1081 gacgtccccg aagggaaccg agtgcgcaag cgtgctggcg cagattgagg cgtacgtcga
     1141 tggtgaactc ggcgccctcc agtgctccgc catcgaacag cactgcgagc agtgcgaacg
     1201 ctgcgccgcc attgttcatg ggttgcgacg aaccattggc ctctgccagg agattggccg
     1261 cgcgccgctg ccgccatcgg tgagccaact ggcgcgagat caggtgaggc gcctgctcga
     1321 gtcttcgcac gattcacctc tgggccgcaa ggctacctga cggcgccgct gcgactacgc
     1381 cgggcgaact attgttcctg ccacagtgtc gatctggcag aatgctccgc gtggagtgcc
     1441 cgcgatgcgg ctcggcgatg cagcaggaga ccttcgacgg ccggctggga cgatcggtgg
     1501 cgatcgacat ctgccacgcc tgccagtcct tctggttcga cacgcgggag agcgtcgcgc
     1561 tcacgccggg agccacgctg gcgctcttcc ggatcatcgg cgagaagctg acgaggcccc
     1621 gacacagcga cgcggagctg gccaagtgcc ctcgctgcaa gggccggctg agacgcaccc
     1681 gcgacatgca gcgcgggacg aagttcgagt acctgaattg cccgaacgga cacggccgcc
     1741 tcatctcgtt cttcgacttc ctgcgcgaga aggacttcat ccgccccctc agtacgagac
     1801 agatcgcgga gctccgcgag aacgtgggcg ccgtcaactg taccaactgc ggaggagcag
     1861 tcgacttgac caagggcacc gcatgcccgc attgcggctc gccgctctcg atgctcgaca
     1921 ttcatcaggc cgagcagctt gtctcgcagc tgcagaaggc cgaagatcgg gccaatcagc
     1981 cggtggaccc gtcgctgccc ctgcagctgc ttcgcgtccg tcgtgaaacc gagcgcgcgt
     2041 tcgccggcct gccgcacgac gacgtctggg tggaggatgt gctgaagacg gatctcgtgg
     2101 gagcggggct gagcgcggtg gcaaggtggc tgaagaggca ctcgtagcag tacggaacac
     2161 ttcgggacgc gttgcgtaat gttcagatag tgcgaaggga gcgaagtgat cgatgggaat
     2221 aatggacttc atcaaagggg agctgctcga gatcatcgag tggaccgacg actccaggga
     2281 cactctgtcg tggcggtttc ctgatgacga caaggccatc aagaacggcg ctcagctcat
     2341 cgtccgcgaa tcgcaggtcg cgcagttcgt ctacctcggc gaattcggcg acacgttcgg
     2401 ccctggcaag catcggctga cgacggacaa catccccgtc ctgacgaaga tcaagtcctg
     2461 gccctacggc ttcaactcgc ccttcaaggc cgacgtctac tacgtcacca cgaggctctt
     2521 caccggcaac aagtggggaa cgtcgaaccc ggtgatggtc cgcgacaacg acttcggcat
     2581 ggtccgcgtg cgcgcgttcg ggacctacga cttcaaggtg atcgatacga agacgtttct
     2641 gaaggaagtg gccggctccg atcacaattt ccgcctcgac gagttcgccg acacgatgcg
     2701 ctcgcgcatc gtcagcatct tcaccgacgc gattgccacg gcgaagattc cggttctcga
     2761 cgtggcgtcg cgctacaccg agctcggcga agcgctgttg ccgctcatca acccggcgat
     2821 caaatccaag tacggtctcg agctcggaag cttcatcgtc gagaacgtgt cggtgccgcc
     2881 ggaagtcgaa gaggcgatcg acaagcgcgc gagcatggcg gctgtgggca atctgaacga
     2941 cttcgtgaag taccagatgg gcaagggatt cgagggcggc ggcggaaccg ggccggcggg
     3001 cacggcggcg gagctcgccg tgggcttcgg cctggcgcag cagatgatga aagagggctt
     3061 catgggggga acggccacgc cgaccgttgc cgccggcgtt cccgccgcgc agccgggcgg
     3121 ggccgcgctg ccggagctgc tcagccctgc cgaagccgcg caggcgctcg gcgtgtccga
     3181 agcggacgtg atgagcgtgc tcgactccgg tgacctcaag ggccggaaga tcggatcgac
     3241 gtggcgcatc tcgcgcgcag cgctcacgag ctacatgtca cagtgagtgc gacgacagag
     3301 atcgcggcac tcgacaagca cgagtgcccg gcctgcggcg gccaggccga gtggaatcca
     3361 ggcaagcaga agctgatttg ccccttctgc ggaaccgagt cgccctacgt catcgaccgc
     3421 gagcagggaa aggtcgtcga gctcgatctc gtcaaggcgc tgcgcgagct gcccgcggac
     3481 gagcggggat ggcagaccga ccggcgcagc gtgcagtgcc agtcctgccg cgccgtgatg
     3541 gtgttcgatc cgtcacgggt cggacagaac tgcgagttct gcggctcgcc ggcgcttgtc
     3601 gattaccagg agatcaagtc tcccatcacg ccgcagggcg tgctgccgtt caggatcgac
     3661 accggccgcg tgcgcgacga catccgacgg tggtggaaga gcaagtggtt tgcgccgggc
     3721 aggcttgcgc gaaccgcgct cgtggacacg atcaagagcc tctacgtgcc gtactggacc
     3781 ttcgacgcgc aggtccattg cccgtggacg gccgacgcgg gctactacta ctacgtcaac
     3841 gttcccggag tcgacgcgaa gggccgcccc gtcgttcgac gcgagcgccg cgtccgctgg
     3901 gagccggccg ctggcgcggt ggacaccttt ttcgacgacg agccggtgcc ggggacccgg
     3961 gggctgccgc tcgacctgtt gaagcaggtc gaaccgttcc cgacgaaaga gctcgtgccg
     4021 tacgacaccg cgtttctgtc cggccacgtc gtcgagcact atcaggtggt gctgctcgac
     4081 gcggcacagc gatcggtcga gcagatgcag gacaggctca tggcgctctg cgcccagcag
     4141 gttcccggcg acacgcatcg aaacctgcgg atcgacccgg cgtattccgg acgcacattc
     4201 aagcacgtcc tcgtccccgt ctggctcctc tcgtacgact acgcgtcgaa ggcctaccag
     4261 gtgatcgtca acgggtatac cgggaagatc gccggacggc atccgtacag cttctggaag
     4321 atcgctttcg cggtgcttct ggccgccatc gcggcgctca tcgtcgtctg gttgaacgcg
     4381 cagcagtagg ggtgcggggg ctgaagcccc cgctctacaa cccaacgcaa gacgccaggc
     4441 cgccgacgca acgcccccga cacgccgccg gcgacacgcc gctcggcgcc cgtagagcgc
     4501 gggcttcagc ccgcgcccag ccgtggtatt ttcgtcgctg gcagtgggag gcacggcaat
     4561 gcggaacctg atcggcgtcg cggcggtggt ggcgatcgct ctagcgtcgg cgccgctgtc
     4621 ggcgcagtgg ccgtcgcatc cgacaccggg cgtgccccgg ctcgagagcg gcgcggtgaa
     4681 catggatgcc cccgtgccgc gcaccgctga cggcaagccc gatctctccg gcaactgggt
     4741 caggttcgcg acgccgggcg gcaggcgcgg agccggccct gcagcgcccg cgccgcctcc
     4801 tcctcccggc acgcccccgg tggccacgtt cggagaggtc ggcgcggggt tccccgaggg
     4861 gcttcccttc acaccctggg ccgccgagct gaagaagcag cggatgtcgg tccagcagaa
     4921 agacaacccc gacgcgttgt gcctgcccat gggtttcatg cagttccaca cgcacggcca
     4981 gccgcggaag gtgatccaga cgccagggct cgtcacgatc atctacgaag ccaattacgg
     5041 actccgctac atcttcacgg acggcaggac tctgccgccg cagggcgacc ctcaaccctg
     5101 gtggtacggc tactcggtcg gccggtggga aggggacacg ctcgtcgtcg agacgaacaa
     5161 ccttcgagac gacggctggc tcgacgtgcg agggagcccg tacaccgatc aggcgaagat
     5221 cacggaacgg ttccgccgtg tgagcttcgg caagctcgag atcgacatca ccatcgacga
     5281 cccgaaggcc tacaccaaac cgttcaccgt cagggtgaac cagcagatct cggtggacaa
     5341 cgagctcatc gagttcatct gcaacgagaa ccagcagttc cggaagctga tcaagatcga
     5401 ctgacgcggg ggctgaagcc cccgctctac gacatggttc ctgtccccgc tctcgccgcc
     5461 cttctcgatc gtctgcgcga gacgaccgac ctgctcgaat ccattgccgc cgatcg
//
LOCUS       DBNGSH010000585        11607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3284404, whole genome shotgun sequence.
ACCESSION   DBNGSH010000585 DBNGSH010000000
VERSION     DBNGSH010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3284404"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="AC16CY_13535"
     CDS             complement(<1..1454)
                     /locus_tag="AC16CY_13535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3354864.1"
                     /translation="MTPDTDLAIVGSGFGGSILAMIARRLGLRVMLLERGRHPRFAIG
                     ESASPLAGILIEQLSDRYGLPRLRPLSAFGTWQRAYPHVRCGLKRGFTYFKHDVGHPY
                     RCAPGRANQLLVAASPSDELSDTHWLRSEVDHFLVDEAAALGAEYLDSTSLERLDIGP
                     DGHPLLTGTRESKPIRVRARFVVDASGPRGFLSRALGIDDRGFDGYPATQALFSHFID
                     VAKCQDMPDYGATVRGCEGATVPYPMDDAALHHVFDGGWMWVLRFGNGVTSAGVAVED
                     WLAADLRIADGAAAWDRLLARYPSVAAQFADAQPIREFTWMPRLPYRAGVAAGERWAM
                     LPSAAAFIDPLFSTGIPLTLLGIERLARILEGGTSEGGGSKDPPLPVRRAGPDRRGGS
                     LDPPNPLRDYNDATLADADLTARFIAGSYAGFRRFGAFAAYSMFYFAAASFSEMARRL
                     SSPRTPKGFLCGDSPAFTSALERLSPAVHTAADIG"
     gene            complement(1438..2382)
                     /locus_tag="AC16CY_13540"
     CDS             complement(1438..2382)
                     /locus_tag="AC16CY_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3354865.1"
                     /translation="MRLARDQFVLTHRAAKTPLSPFHPYASVWEEEPDTSRALAPTAV
                     IFLTNRECPFRCVMCDLWINTLDETVPPGAIPAQIREALSRLAPIRQVKLYNAGSYFD
                     PRAIPPEEDEAVAREVDACTRVIVESHPAFLAGAHAERCLRFRDLLSGQLEVAIGLET
                     AHPGVLDRLNKQMTIDTFRHAADFLAEHDIALRVFVLLGPPYMPAADAVEWACRSIEL
                     AGECGATACTVIPTRGGNGAMEALDPPFAPPRLPSLEAAVEYGLHRASGMRVFADLWD
                     IERFFDCGCAGHRAARLRVMNREQRVPPRVVCACDARH"
     gene            complement(2366..3715)
                     /locus_tag="AC16CY_13545"
     CDS             complement(2366..3715)
                     /locus_tag="AC16CY_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR3354866.1"
                     /translation="MVERLVDLIGTRTAALTGGSEAEVASWTASCDVAALGATDGHFA
                     SVGRDGRTVRLARTIGLPLRYFVAKMYHGPFLVVADRMDRIFEWCRSERIAWQFEPAY
                     TRMVPAHYLVELDQVGCPDPAPRYHRFFDPPVALGSADIDEAGAAYVSAAFRALKEWL
                     GRVPEREPVAVAFSGGIDSLSVLLLARQACVELGRSPDQIKAFTLDLGGGEDAAQAER
                     AVHHLGLASSWEAVAASPAECDLEGAIRLIEDYQPLDVECAATATCLLRGVRRRYPGL
                     KYLLDGDGGDENLKSYPLEDSDLTLSSVLRNPLLYQEGWGIDAIKHSLAYSGGLSRSY
                     VRTYAPAAALGFEAFSPFTVRSVVAAAVAIPFERVLGGREQRLYTLKQDVVSAGIKAA
                     LGVEMPTNPKRRFQDGAGAPRARVSKAWCRRVFSRIWQDRLKEAEALERRSGNEVGA"
     gene            complement(4267..4803)
                     /locus_tag="AC16CY_13550"
     CDS             complement(4267..4803)
                     /locus_tag="AC16CY_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3354867.1"
                     /translation="MSIDPRAFRQTLGLFVTGVTVIAMEVDGSIRALTANSFTSLSLN
                     PPLVLFCLGKGTKAGQVIEQLREFSVNILSQEQRALSTYFAGSWKGSEPPPFTFTRSE
                     GGPRLDGCVATLGCTVNAVHEGGDHWIVVGQVVTLHRSDLPRAPLVFFSGRYSTLSGG
                     VTSAEEPGLLISWTEPEQ"
     gene            complement(4871..6433)
                     /locus_tag="AC16CY_13555"
     CDS             complement(4871..6433)
                     /locus_tag="AC16CY_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3354868.1"
                     /translation="MAADTTGRTRPYTGAEYLESIRDGREIWIYGERVKDVTTHPAFR
                     NTARMIARLYDALHDPARKDVLTTPTDTGSGGFTHKFYKASRNAEELVGARDAIAEWA
                     RVTYGWIGRSPDYKAAFLATLGANASYYAPYDENARRWYKKVNDEVSFVNHAIVNPPV
                     DRDKPLDEVKDVYMHVEEETDAGLVVSGAKVVATTSTLTHFNFIANNGALPIKTKPFA
                     FVCMVPTDAKGVKLFCRPSYEMTAAVMGTPFDYPLSSRMDENDSILVFDKVLVPWENV
                     FAYGDLEKVNNFFPRSGFLPRFMFQGCTRLAVKMDFLAGLLLKAVEATGAKDFRGVQA
                     QVGEVLAWRNLFWGLTDAMARTTIPWTEGYVLPNLDYGLAYRVMASLAYPKIKEIIEN
                     TLASALIYLPSSALDFKVPELRPYLDQFVRGSNGYSAEDRVKLMKLMWDAIGSEFGGR
                     HELYERNYFGNHESIRFETLLVADVTGATKRYKGFAEQCMAEYNLDGWTVPDLVRSDD
                     VSAIVKNIGTRG"
     gene            complement(6439..7299)
                     /locus_tag="AC16CY_13560"
     CDS             complement(6439..7299)
                     /locus_tag="AC16CY_13560"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3354869.1"
                     /translation="MHYYGSFFPDGLGHHPLYEDHSAGYTHESLIGPETGSVHTGLSV
                     NELAPGGTIDPHVHSYEEGFYLLGGQAIVTIGGSSHLLGTGDYAAVKVGTPHAWRAVG
                     STPARWLQMASPQPKPLGAERDTFFLKDRSIPQSAPPLDLKDVKGNLLGHFDAGQIPP
                     PGQRPPAVTGLEGVFLKWLIDEDFGARHHRLLFIEYQPGVTLGLHDHTFEEAYFILSG
                     EVQGTLDGQTYIGKPGDVFWTGVGCVHGFANVGTQPVTWLETFAPQPPKENVFRFMAE
                     WERRARELEG"
     gene            complement(7307..7921)
                     /locus_tag="AC16CY_13565"
     CDS             complement(7307..7921)
                     /locus_tag="AC16CY_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HIR3354870.1"
                     /translation="MRVFELRVALTSAGLLCAVAIAGCGQPASAPPPASAPAAATAES
                     QIERGRLLIAGGACHDCHSPKVFTPKGPEPDKARLLSGHPESDKITAPYKQQPGEKWS
                     THANDHLTAWSGAWGVSFAANLTPDQNTGIGIWTEDMFVEAIKSGKHMAKGRQILPPM
                     PWMFYGQLHDDDLKAMFAYLKSLPPISNRVPVPLGPDGKPIEEP"
     gene            8086..9807
                     /locus_tag="AC16CY_13570"
     CDS             8086..9807
                     /locus_tag="AC16CY_13570"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR3354871.1"
                     /translation="MPGLLRYIFSTDHKVIGLQYGFTSLFFLLVGFALVMLIRWQVAY
                     PGQPIPLAGLLGEANAPGGVLLPEFYNQLGAMHGTIMVFLGIVPLAFGAFGNYLIPLQ
                     IGAPDMAFPRLNALSYWLYLAGGIVMLVSFGVPGGAANNGWTSYAPLSDIAPGHGQTL
                     WLAGMLLIILSSLLGAMNFITTIVQLRAPGMTWFRLPFFVWAQFVTAFLLLLAFPPLQ
                     GAAVLQAMDRLASTSFFIPSGLVVGGQPLDVAGGGNALLWQHLFWFLGHPEVYVLILP
                     ALGIVAEVVANNTRKPLWGYRLIVYSLFVLGFLSFVVWAHHMFLTGMGTAISAFFQAT
                     TMIISIPSVIILSALILSLYGGSIRFTTPMLFALAFMPMFGIGGLTGLPLGLSATDIP
                     LHDTNYVVAHFHYLVAPGTIFAMFAGIYYWFPKITGRMMNDTLGRIHFAGSLVSMNVI
                     FMPMFVQGLAGVNRRLWDGGALYSHAQETLSLNVPMSYAALVLGVFQIFFVVNLVWSL
                     RAGRVVDANPWEATTLEWSAAPTPPRAHGNFVTPPRVVRDPYEYSVPGAAAGFLPQAA
                     TDAESRT"
     gene            9808..10443
                     /locus_tag="AC16CY_13575"
     CDS             9808..10443
                     /locus_tag="AC16CY_13575"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR3354872.1"
                     /translation="MGTLPYTTDVRPDTGLTNPKLGIWLFLASEVMLFGALFSSYVLL
                     RIGALEWPDQSSVVSVPFGAFNTVVLIASSVTMVLAWASLASRLPGARRGDAQAQAAG
                     LGRYRLFMGLTLLAGAIFLVVKAFEYSDKFSHHLYPSTNVFLGLYFTMTGLHALHVII
                     GMIVNAYLWGPGARMWRSEPERFTNRIEVAGIYWHFVDVVWIFLFPVLYLL"
     gene            10493..10819
                     /locus_tag="AC16CY_13580"
     CDS             10493..10819
                     /locus_tag="AC16CY_13580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR3354873.1"
                     /translation="MTHDVAVQVRKYLIVFAALLALTVITVGVSYLQLPRVETVTLAL
                     VIATAKASLVAMFFMHLKGERPMVMWPLSLTAFLFVAMLGFLLMSEADHLFGTKFGDA
                     FQNIFD"
     gene            10890..11189
                     /locus_tag="AC16CY_13585"
     CDS             10890..11189
                     /locus_tag="AC16CY_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354874.1"
                     /translation="MVLLIGAQSTINNRQSTSNRQSTITDRQIMSCPVCFGGEDAAMR
                     ESLNAGIGVLMGVTAVVLGCFARFFVMLARRADKWGRESFIDDTGASLTDTVLNK"
     gene            11186..>11607
                     /locus_tag="AC16CY_13590"
     CDS             11186..>11607
                     /locus_tag="AC16CY_13590"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3354875.1"
                     /translation="MTPDPIFQPMLQWLGMPVGASAHAGEVDRIMALVHWLMLVLFVG
                     WSIFFVYVLARFRRGRNPQANYHGVRGRWSTWVEAGVLVAEIVLLAFFSIPFWSTNVD
                     ALPAEHESTIVRVVAEQFAWNVHYPGADGVFGRTDIRLV"
BASE COUNT         1855 a         3862 c         3803 g         2087 t
ORIGIN      
        1 ccgatgtctg cggccgtatg caccgccggc gacaggcgct cgagggcgga cgtgaacgcg
       61 gggctgtcgc cgcacaagaa acccttcggc gtgcgtggag atgacagccg gcgggccatt
      121 tcgctgaagc tcgccgcggc gaagtagaac atcgagtagg cggcgaaggc gccgaaccga
      181 cggaacccgg cgtagctgcc ggcgatgaag cgcgcggtca gatccgcgtc ggccagcgtg
      241 gcgtcgttgt agtcgcgcag aggattaggc gggtctaaag acccgcctct acgatccggg
      301 cccgcacgtc gtaccggtag aggcgggtct ttagacccgc ctccctccga ggtcccgcct
      361 tccagtattc gcgccaaccg ttcgatcccg agcagcgtga gcggaatccc cgtggagaac
      421 agcggatcga tgaatgccgc cgccgatggc agcatggccc atcgctcccc cgccgccacg
      481 ccggcgcggt agggcaggcg cggcatccag gtgaactcgc gaatcggctg cgcgtcggcg
      541 aactgggcgg ccacggacgg gtatcgggcg agcagccggt cccaagctgc ggcgccgtcg
      601 gcgatccgca gatcggcggc gagccagtct tcgacggcga ctccggcgct cgtgacgccg
      661 ttgccaaagc gtagaaccca catccatccc ccgtcgaaca cgtggtgtaa cgctgcatcg
      721 tccatgggat acggcaccgt cgcaccttcg caccctcgca ccgtcgcacc gtagtccggc
      781 atgtcctgac acttcgcgac gtcgatgaag tgcgaaaaga gcgcctgagt cgccggatag
      841 ccgtcgaagc cgcgatcgtc gatccccaga gccctgctca ggaacccacg cggcccggac
      901 gcgtccacga cgaacctcgc gcggacgcgt atgggcttcg actcgcgcgt gccggtgagg
      961 agggggtgtc cgtccggacc gatgtccagc ctttcgagag aggtcgaatc caggtactcg
     1021 gcgcccagcg ccgcggcctc gtccacgaga aagtgatcga cctcgctgcg aagccaatgg
     1081 gtgtcagaca gttcgtcgct cgggctcgcc gccacgagca gctgattggc ccgtcccggc
     1141 gcgcatcggt acggatgacc gacatcgtgc ttgaaatacg tgaacccccg tttcagcccg
     1201 caccttacgt gcggatacgc ccgctgccac gtgccgaacg ccgagagcgg ccggaggcgc
     1261 ggcaacccat accgatccga cagctgctcg atcaggatgc ccgccagggg cgacgccgac
     1321 tcgccgatgg cgaatcgggg atggcggccg cgctcgagga gcatcacgcg gagcccgagg
     1381 cgcctggcga tcatcgcgag gatcgagccg ccgaaaccgg agccgacgat ggcgaggtca
     1441 gtgtcgggcg tcacaggcgc acacgacgcg cgggggcacg cgctgctcgc ggttcatgac
     1501 ccgaaggcgc gcggcgcggt ggcccgcgca gccgcagtcg aagaaccgct cgatgtccca
     1561 cagatcggca aacacacgca tcccgctcgc cctgtgaagg ccgtactcca cagccgcctc
     1621 gagcgagggt agcctcggcg gcgcaaacgg cgggtcgagc gcctccatgg ccccattgcc
     1681 tccgcgcgtg ggaatgacgg tgcacgccgt ggcgccgcac tcgcccgcga gctcgatcga
     1741 acggcacgcc cattcgacgg cgtctgcagc aggcatgtac ggcgggccga gcagcacgaa
     1801 cacacgcagt gcgatgtcat gctccgcgag aaagtcggcg gcgtggcgga acgtgtcgat
     1861 ggtcatctgc ttgttgagcc ggtcgagcac tcccgggtgc gccgtctcga gcccgatcgc
     1921 cacctcgagc tgccccgaga gcaggtcgcg gaagcgcagg catcgctccg cgtgcgcccc
     1981 ggcgagaaac gctggatgcg actcgacgat gacacgcgtg caggcgtcca cctcgcgcgc
     2041 gaccgcttcg tcctcttcag gagggatggc gcggggatcg aagtagctgc ccgcgttgta
     2101 cagcttgacc tggcggatcg gcgccagccg cgacagcgcc tcgcgaatct gcgcggggat
     2161 ggcgccgggc ggcacggtct cgtcgagcgt gttgatccag agatcgcaca tcacgcagcg
     2221 gaagggacac tcgcggttgg tgaggaagat caccgcggtg ggagccagcg cacgcgacgt
     2281 gtcgggctct tcttcccaga cggatgcgta cgggtgaaag gggctgagcg gcgtcttggc
     2341 cgctcgatga gtcaggacga actggtcacg cgccaacctc attacccgat ctccgctcca
     2401 gcgcttccgc ttccttcagc ctgtcctgcc agatcctgct gaacaccctg cggcaccagg
     2461 ctttgctcac cctggcgcgc ggcgcccccg cgccgtcctg gaagcgccgc tttggattgg
     2521 tcggcatctc gacgccgagc gcggccttga ttcccgcgct cacgacgtcc tgcttaagcg
     2581 tgtacagtcg ctgctcgcgg ccgccgagca cccgctcgaa tggaatggcg accgccgcgg
     2641 cgacgaccga ccggacggtg aacggcgaga acgcctcgaa cccgagcgcg gccgccggcg
     2701 cgtaggtgcg gacgtagctg cgggagagtc cgcccgagta ggcgagactg tgcttgatgg
     2761 cgtcgatgcc ccagccctcc tggtagagca gcgggttgcg caggacgctc gacagcgtga
     2821 ggtccgagtc ctcgagcgga tacgacttga ggttctcgtc gccgccgtcg ccgtcgagca
     2881 ggtatttcag gccgggatag cgccggcgca cgccgcgcag caggcacgtc gccgttgccg
     2941 cgcattcgac gtcgagcggc tggtagtcct cgatgaggcg gatggcgcct tcgaggtcgc
     3001 actcggcagg cgacgctgcg acggcctccc agctggaggc gagccccagg tgatgcaccg
     3061 cgcgctcggc ctgcgcggcg tcctctcccc cgccgaggtc gagcgtgaac gccttgatct
     3121 gatcggggct gcggccaagc tccacgcacg cctgcctggc cagcagcagc accgacaagc
     3181 tgtcgattcc tccggaaaac gcgacggcca ccggctcgcg ctcgggcacg cggcccagcc
     3241 actccttcag ggcgcgaaag gccgccgata cgtacgcggc gccggcttcg tcgatgtccg
     3301 cggagccgag cgccacgggg ggatcgaaaa agcggtggta acgaggggcg ggatcggggc
     3361 atccgacctg atcgagctcc accaggtaat gcgccgggac catgcgcgtg taggccggct
     3421 cgaactgcca ggcgatgcgc tcgctccggc accactcgaa gatgcgatcc atccggtcgg
     3481 ccacgacgag gaacgggccg tggtacatct tcgccacgaa gtaccgcagc ggcaggccga
     3541 tcgtcctcgc gaggcggacc gtccgtccgt cgcgcccgac cgaggcgaag tgtccatcgg
     3601 tggcgccgag cgcggccacg tcgcaactgg ccgtccacga ggccacctcc gcttcggagc
     3661 cgcccgtcag cgccgcggtc cgagtgccga tgagatctac cagtcgctcg accattcgtg
     3721 atcgttgatt ataggggtcg tcgcccgtta cagccgcccc gcccgacgtg tcccttccgc
     3781 gcggcgggtc gcgacgcccg gccaccagca tctccggcgt cagcctgacg gcccgtcggc
     3841 gctccgccgt cgcgatcgtc tgtcgccctc gcttctcccc cgcgcctcca cgcctgcgat
     3901 gccggtggcg agctatcgta cgacgggctt gcgacacgcc gtcgcgtcag gacacatcag
     3961 gcgggccggc tgcctgtctc agcaccgcgc catccagcca cgcctgtctg tcgggctggc
     4021 tggcgcctgc gcaaacgccc gttcactgcg aagcgggaaa tcggctgatg cgagccttcc
     4081 gcggcgagtt cgacttccgc gttctggttg tgccgagacc acggtcgttt cgtgccgcgt
     4141 aacagtcgtc gtggcgaatc agctcctact cgccgcgtca gcgaaagggc gacgagcaga
     4201 agccgatttc cggcgtgccg cgccggcgcc gccggaccga caggcaggag tggctggatg
     4261 gctcgtctat tgttccggtt cggtccatga aataagcagc ccaggctctt cagccgacgt
     4321 gacgccgccg gacagcgtgg agtagcggcc gctgaagaac accagcggcg cgcgcggcag
     4381 atcgctgcga tggagggtca ccacctgacc gacgacgatc cagtggtcgc ccccctcgtg
     4441 cacggcgttg accgtgcagc cgagcgtggc gacgcagccg tcgagccgcg ggccgccctc
     4501 ggagcgcgtg aaggtgaagg gcggcggctc gctgcctttc cacgatccgg cgaagtaggt
     4561 cgagagcgcc cgctgctcct ggctgagaat gttgaccgag aactcgcgca gttgctcgat
     4621 cacctgcccg gccttggtgc ctttccccaa acagaagagc acgagcggcg ggttgagcga
     4681 cagcgacgtg aacgagtttg ccgtcagcgc gcggatcgag ccgtcgacct ccatcgcgat
     4741 gacggtcacg cccgtcacga agaggccgag cgtctgacgg aacgcccgcg gatcaatgga
     4801 catgaacacg aaccccgaaa ctgaacacga accgagaagc gagaaccgag aagtgtgaac
     4861 gaccggtcct ctatcccctc gtcccgatat tcttcactat cgcgctgacg tcgtcggatc
     4921 gcacgaggtc gggcacggtc cagccatcga ggttgtattc ggccatgcac tgctccgcga
     4981 accccttgta gcgcttcgtg gcgccggtga cgtcggccac cagcagcgtc tcgaagcgga
     5041 tgctctcgtg gttgccgaag tagttccgct cgtacagctc gtggcgtccg ccgaactcgc
     5101 tgccgatcgc atcccacatc agcttcatca gcttgacccg gtcctccgcc gagtagccgt
     5161 tggagccgcg cacgaactga tcgagatacg ggcgcagttc ggggaccttg aaatcgagcg
     5221 cgctcgacgg caagtagatc agcgcgctgg ccagtgtgtt ctcgatgatc tccttgatct
     5281 tcgggtacgc caggctcgcc atcacgcgat acgccagccc gtagtcgagg ttcggcagca
     5341 cgtacccctc ggtccacgga atcgtcgtcc gcgccatcgc gtcggtcagc ccccagaaca
     5401 ggttccgcca cgccagcact tcgcccacct gggcctggac gccgcggaag tccttcgcgc
     5461 ccgtcgcttc caccgccttg agcagcaacc cggccaggaa gtccatcttc acggcgaggc
     5521 gggtgcaacc ctggaacatg aagcgcggga ggaaccccga gcgcggaaag aagttgttca
     5581 ccttctcgag atcgccgtag gcgaacacgt tctcccaggg gacgagcacc ttgtcgaaga
     5641 cgagaatcga gtcgttctcg tccatgcggc tcgacagcgg gtagtcgaac ggcgtgccca
     5701 tcacggccgc cgtcatctcg tacgacgggc ggcagaacaa cttcacgccc ttcgcgtcgg
     5761 tcggcaccat gcagacgaag gcgaacggct tggtcttgat cggcagcgcg ccgttgttgg
     5821 cgatgaagtt gaagtgcgtc agcgtggacg tcgtggccac gaccttcgcg ccgctgacga
     5881 cgaggccagc gtcggtttcc tcctcgacgt gcatgtacac gtccttcact tcgtcgagcg
     5941 gcttgtcgcg atcgaccggc gggttcacga tcgcgtgatt gacgaaggag acctcgtcgt
     6001 tgaccttctt gtaccagcgg cgggcgttct cgtcgtacgg cgcgtagtac gacgcgttgg
     6061 ccccgagcgt cgccaggaac gccgccttgt agtcggggct gcgcccgatc cacccgtagg
     6121 tgacgcgcgc ccactccgcg atcgcgtccc gcgcgccgac gagctcctcg gcgttgcgcg
     6181 aggccttgta gaacttgtgc gtgaagccgc cgctgccggt atctgtcggc gtcgtcagga
     6241 cgtccttgcg cgcggggtcg tgaagggcgt cgtacagccg ggcgatcatg cgggccgtgt
     6301 tgcgaaacgc ggggtgcgtc gtcacgtcct tgacccgctc gccgtagatc cagatctcgc
     6361 ggccgtcccg gatgctctcc aggtattcgg cgccggtgta ggggcgcgtg cgccccgttg
     6421 tatcagccgc catgatgact acccctccag ttctcgagcg cgacgctccc actccgccat
     6481 gaagcgaaac acgttttcct tcggcggctg gggcgcgaag gtctccagcc aggtgaccgg
     6541 ctgcgtgccg acgttggcga acccgtggac gcagccgacg ccggtccaga acacgtcccc
     6601 aggcttgccg atataggtct ggccgtccag cgtgccctgc acttccccgc tgagaatgaa
     6661 atacgcctcc tcgaaggtgt ggtcgtgcag tccgagcgtg acgccgggct gatactcgat
     6721 gaacaacagc cgatggtggc gcgcgccgaa gtcttcgtcg atgagccact tcaggaacac
     6781 gccctcgagc ccggtgacgg cgggcggacg ctgcccggga ggcggaatct ggccggcgtc
     6841 gaagtgaccg agcaggttgc ctttcacgtc cttcaggtcg agcggcggcg ccgactgcgg
     6901 aatcgatcga tccttcagga agaacgtgtc gcgttcggcc cctaacggct tcggctgggg
     6961 cgacgccatc tgaagccagc gtgccggggt ggagcccacg gcgcgccagg cgtgcggcgt
     7021 accgactttc accgcggcgt agtcgcccgt gccgagcaga tggctcgacc cgccgatcgt
     7081 gacgatcgcc tgcccgccga gcaggtagaa gccttcttcg taggaatgga cgtggggatc
     7141 gatggttccg cccggggcaa gctcgttgac gctcagcccc gtgtgcaccg agccggtttc
     7201 cgggccgatc agcgactcgt gtgtgtagcc cgcgctgtga tcctcgtaaa gcggatgatg
     7261 accgagcccg tcggggaaga agctgccgta gtagtgcatg ccggcatcac ggctcttcga
     7321 tcggcttccc gtccggaccg agcggcacgg gcacccggtt cgagatcggg ggaagcgact
     7381 tcagatacgc gaacatcgcc ttgaggtcgt cgtcgtggag ctggccgtaa aacatccacg
     7441 gcatcggggg gaggatctgg cgccccttgg ccatgtgctt cccactcttg atcgcctcga
     7501 caaacatgtc ctcggtccag atgccgatgc cggtgttctg atccggtgtc aggttcgcgg
     7561 caaacgacac tccccaggca ccactccacg ccgtcagatg atcgttggcg tgggtcgacc
     7621 acttctctcc gggctgctgt ttgtagggcg ccgttatctt gtcgctttca ggatggccgg
     7681 acagcagacg cgccttgtcg ggttctggcc ccttgggcgt aaagaccttc ggcgagtggc
     7741 aatcgtgaca ggctccaccg gcgatcagca ggcggcctcg ttcgatctgg gactcagcag
     7801 tcgccgctgc cggcgccgac gcgggcgggg gcgcgctggc cggctgaccg catcctgcga
     7861 tcgcgactgc gcacaacaaa ccggccgacg tcagggcaac acgtaactcg aaaacacgca
     7921 ttggaaatcg actcctcgat gaactcgctg cgcgcgaagg ccttcgctgc tagcgaaggc
     7981 ctgcaatata caccaggatt gaggtgcgag ggtgcgacgg tgcgagggtg cgacggtgcg
     8041 agggcgcgaa ggccaccttg ccctcgcgca attggcgtag gtaagatgcc gggattgctc
     8101 cggtatattt tttcaaccga tcacaaggtc atcggccttc agtacgggtt caccagcctc
     8161 ttcttcctgc tggtcggctt cgcgctcgtc atgctcattc gctggcaggt cgcctaccct
     8221 ggccagccga tcccgctcgc cggactgctg ggtgaagcga acgcgcccgg cggcgtcctg
     8281 cttcccgagt tctacaacca gcttggcgcg atgcacggca ccatcatggt gtttctcggc
     8341 atcgtgccgc tcgccttcgg cgccttcggc aattacctca tcccgctgca gatcggcgcg
     8401 ccggacatgg cgtttccacg cctcaacgcg ctgagctact ggctgtacct ggccggcggc
     8461 atcgtgatgc tcgtcagctt cggcgtgcct ggcggcgccg ccaacaacgg ctggacgtcc
     8521 tacgctccgt tgtccgacat cgcgccgggc cacggccaga cgctctggct ggccgggatg
     8581 ctgctcatca tcctgtcgtc gctgctcggg gcgatgaact tcatcacgac gatcgtgcag
     8641 cttcgcgcgc cgggcatgac atggttccgc ctgccgtttt tcgtgtgggc gcagtttgtc
     8701 accgcgttcc tgctgttgct ggcgtttccg ccgctgcagg gggcggccgt ccttcaggcg
     8761 atggaccgcc tggcgtccac gagcttcttc attccgagcg gtctcgtggt cggcggtcag
     8821 ccgctcgacg tcgccggcgg cggtaacgcc ctgctgtggc agcacctgtt ctggttcctc
     8881 ggtcatcccg aggtgtacgt cctcatactt ccggcgctcg gcatcgtcgc ggaagtggtc
     8941 gccaacaaca cgcgaaagcc gctctggggc taccggctga tcgtgtactc gctgttcgtc
     9001 ctcggcttcc tgtcgttcgt cgtctgggcg caccatatgt tcctgacggg catgggcacc
     9061 gcgatcagcg ccttcttcca ggcgacgacg atgatcatct cgattccttc ggtcatcatc
     9121 ctgagcgcgc tgattctctc gctctacggc ggctcgatcc gcttcacgac gccgatgctg
     9181 ttcgccctcg ccttcatgcc gatgttcggg atcggcgggc tgacggggct tcctctcggc
     9241 ctgtcggcca ccgacattcc gctgcacgac accaactacg tggtggcgca cttccactac
     9301 cttgtcgcgc cggggacgat cttcgcgatg tttgccggca tctactactg gttcccgaaa
     9361 atcacgggcc ggatgatgaa cgacacgctc ggccgcattc acttcgccgg ctcgctcgtg
     9421 tcgatgaacg tcatcttcat gcccatgttc gtccagggcc tggcgggcgt gaaccggcgg
     9481 ctctgggacg gcggcgcgct ctactcgcac gcgcaggaga cgctgtcgct gaacgtgccg
     9541 atgtcgtatg ccgcgctggt actcggcgtc tttcagatct tcttcgtcgt caacctggta
     9601 tggagcctgc gcgccggccg cgtggtagac gccaacccgt gggaggcgac cacgctggag
     9661 tggtccgccg cgccgacgcc gccccgggcg cacggcaact tcgtcacccc cccgcgcgtc
     9721 gtccgggacc cgtacgagta cagcgtgccg ggggccgccg ccggttttct gccccaggcc
     9781 gccacggacg ccgagtcgag aacctgagtg ggcacccttc cctacaccac ggacgtccgg
     9841 cccgataccg gactgaccaa tcccaaactg ggaatctggc tgtttctcgc gtcagaagtg
     9901 atgctgttcg gtgcgctctt ctcgtcgtac gtgctcctgc gcatcggcgc cctcgaatgg
     9961 ccggatcagt cctctgtggt cagcgtgccc ttcggcgcct tcaacaccgt cgtgctgatt
    10021 gcctcgtcgg tgacgatggt gctggcgtgg gcatcgctgg cgtcacggct ccccggcgcg
    10081 cgccggggag acgcgcaggc gcaggcggcc ggacttggcc gctaccgcct gttcatgggg
    10141 ctgaccctgc tggcgggcgc gatcttcctg gtggtcaagg ccttcgagta cagcgacaag
    10201 ttctcgcacc acctgtatcc gtcaacgaac gtcttcctcg gcttgtattt cacgatgacg
    10261 gggctgcacg ccctgcacgt gatcatcggc atgatcgtca acgcctacct gtggggaccg
    10321 ggcgcccgga tgtggcggtc cgagccggaa cgcttcacca accggatcga ggtagccgga
    10381 atctactggc atttcgtgga cgtcgtctgg atctttctat ttcccgtgct gtatctgctg
    10441 tgaggaccgc gggggctgaa gcccccgctc tacgtgatga cgcactgacg ccatgaccca
    10501 cgacgttgcc gttcaggtga ggaagtatct gatcgtgttc gcggccctgc tggcgctgac
    10561 agtcatcacg gtgggcgtgt cgtacctgca gctgccgcgg gtcgagacgg tgacgctcgc
    10621 gctcgtgatc gccacggcga aggcgtcact ggtggcgatg ttcttcatgc acctcaaggg
    10681 cgagcggccg atggtcatgt ggccgctgtc actgacggcg tttctcttcg tggcgatgct
    10741 cgggttcctg ctgatgagcg aggccgacca tctgttcgga acgaagttcg gggatgcgtt
    10801 tcagaacatt tttgattagt gatctttgat ttcttgattc gttggtgatg gttgaatgtt
    10861 gattgttgat tcattgggat tgttggcggg tggtgctctt gatcggagcc caatcgacaa
    10921 tcaacaatcg ccaatcaaca agtaatcgac aatcaacgat cacagatcgg caaatcatgt
    10981 cctgtccggt gtgtttcggc ggcgaagacg cggcgatgcg ggagagcctg aacgccggca
    11041 tcggcgtcct gatgggcgtg acggcagtgg tgctcggctg cttcgcccgc tttttcgtga
    11101 tgctcgcgcg gcgcgccgat aaatggggtc gggagtcatt tattgatgac accggtgctt
    11161 cactcacaga cactgttctc aataaatgac tcccgacccc atttttcagc ccatgctcca
    11221 atggctgggt atgcccgttg gcgcctccgc ccacgcaggc gaagtggacc ggatcatggc
    11281 gctcgtgcac tggctgatgc tggtgctgtt cgtgggctgg tcgatcttct tcgtgtacgt
    11341 cctggcgcgc ttcaggcgcg gcaggaatcc gcaggccaac taccacggcg tgcgcggccg
    11401 atggtcaacc tgggtcgagg ccggcgtgct ggtcgccgag atcgtcctgc tggccttctt
    11461 ctcgatcccg ttctggagca ccaacgtgga cgcgctgccc gcggagcacg agagcaccat
    11521 cgtgcgtgtc gtggccgagc agttcgcgtg gaacgtccac tacccggggg cggacggcgt
    11581 gttcggccgc acggacatcc ggctcgt
//
LOCUS       DBNGSH010000586         1748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_58700, whole genome shotgun sequence.
ACCESSION   DBNGSH010000586 DBNGSH010000000
VERSION     DBNGSH010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_58700"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="AC16CY_13595"
     CDS             complement(<1..428)
                     /locus_tag="AC16CY_13595"
                     /inference="COORDINATES: protein motif:HMM:NF014042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR3354876.1"
                     /translation="MRLRRLNVGCVGGGTGLPSLIGGLKANPWLHLNAVVTMFDSGGS
                     SGQLRDELGVLPPGDVLKCALALARNEREARNVLLSRLPTLEADDRLAGHTGGNLLLS
                     MMVRYSGDFLAAVDGLRALLGCRGRVFPVSVEAASVCAQYA"
     gene            524..1231
                     /locus_tag="AC16CY_13600"
     CDS             524..1231
                     /locus_tag="AC16CY_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354877.1"
                     /translation="MASAWKNSTASLVDDLRRTFGDRLRSVVAYGPILDGTADAPLTC
                     LALVSSLTADDLQSCARFASRWERQQIATPLILPEREFMRSLDTFPLEYGEIIRAHER
                     VFGDDPFSAATISPDDLRRACELQTKSHLVHLREAYIETGGQPAAVARLVAASAPAFA
                     ALLRNVARLNRVDTRDRVDATRAGALAVGLSEGLVTDVLNLERPTGVPLNDPSRLFPE
                     YLAAVEQLARAVDAWRT"
     gene            1312..>1748
                     /locus_tag="AC16CY_13605"
     CDS             1312..>1748
                     /locus_tag="AC16CY_13605"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR3354878.1"
                     /translation="MRVLGGLLFVCVVAFPVFAQEAPPQLTRAVNDFAGVIDGESERA
                     IEALIRSLQQASGDVVVVAAVDTFAPYADIQEYAVRMFENRGRGVGQRGRDNGLLIVL
                     AVKDRRVHVEVGYELEQFITDGFAGETSRRDMAPEFKQGNYGRG"
BASE COUNT          271 a          612 c          581 g          284 t
ORIGIN      
        1 gcgtactgcg cgcacactga cgcggcttcc acgctgaccg ggaacacccg cccgcggcac
       61 ccaagcagtg cgcgaagtcc gtccacggcc gcgaggaagt cgccgctgta acggaccatc
      121 atcgagagga ggaggttgcc gccggtgtgc ccggcgagcc gatcgtccgc ttcgagcgtc
      181 ggcagccgtg acaacagaac gttgcgcgcc tcgcgctcgt tcctcgccag ggccagcgcg
      241 cacttcagga cgtcgcccgg cggaagcacg ccaagctcat cccgcagctg gccggagctg
      301 ccgccgctgt cgaacatcgt gaccacggcg ttcaggtgca gccaggggtt cgccttgagg
      361 ccgccgatca ggctgggcaa ccccgtgccg cccccgacac agccgacgtt cagcctccgg
      421 agtcgcattc gtcgcattgt actattcgga acctttgccc tccggctgca gtcttatctt
      481 ggtgatgagt aaggccggga gccctgagcc gtacaatctc gccgtggcct cagcatggaa
      541 gaactcgacg gcatcgctcg tggacgatct ccggcgcacc ttcggcgacc gtcttcgctc
      601 ggtcgtcgcg tacgggccga tcctcgacgg gaccgccgac gcgccgctca cgtgcctggc
      661 gctcgtcagc agcctgacgg ccgacgacct tcagtcgtgc gcccggttcg cctctcgctg
      721 ggagcggcag cagatcgcca ctccgctcat cctgcccgaa agggagttca tgcgatcgct
      781 cgacacgttt ccgctcgagt acggagagat catccgcgcg cacgagcggg tgttcgggga
      841 cgatccgttc agcgccgcga cgatttcacc cgacgacctc cggcgcgcgt gcgaactgca
      901 gaccaagagc cacctcgtcc acctgcgcga ggcgtacatc gaaaccggtg gccagcccgc
      961 agccgtggcc aggctcgtcg ccgcgtcggc tcccgccttc gcggctctgc tgcggaacgt
     1021 cgcgagactc aaccgcgtcg atacacgtga ccgtgtggac gcgacgcgag ccggtgcact
     1081 ggcggtggga ttatccgaag ggctggtcac cgacgtgctc aacctggaac gcccgaccgg
     1141 cgtcccgttg aacgacccgt ctcgcctctt ccccgagtac ctggccgccg tggagcagct
     1201 cgcccgcgcg gtcgacgcgt ggcggacctg atcatcgtca ggccccaggg cccggggctc
     1261 aggcctcagc tcggactctc tggagcctgg ggcccgaggc ccggggcctg cttgcgtgtt
     1321 cttggtggcc ttctcttcgt gtgcgtcgtg gcctttccag tattcgcaca ggaagcgccg
     1381 ccccagctca cgcgcgcggt caacgatttc gcgggcgtca tcgacggcga gagcgaacgt
     1441 gcgatcgaag cgctcatccg ctcgctccag caggccagcg gggacgtcgt cgtggtggcc
     1501 gcggtcgata cgttcgcgcc gtacgcggac atccaggagt atgcggtccg catgttcgag
     1561 aatcgcggcc gcggcgtcgg acagcgcggt cgcgacaacg ggctgctcat cgtgctggcg
     1621 gtcaaggatc gccgggtgca cgtcgaagtc ggctacgagc tcgagcagtt catcaccgac
     1681 ggctttgcgg gcgagacgag ccgcagggac atggcgccgg agttcaagca gggcaactac
     1741 gggcgcgg
//
LOCUS       DBNGSH010000587         1703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_908347, whole genome shotgun sequence.
ACCESSION   DBNGSH010000587 DBNGSH010000000
VERSION     DBNGSH010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_908347"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC16CY_13610"
     CDS             <1..333
                     /locus_tag="AC16CY_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354879.1"
                     /translation="RWEGDTLVVETSNFIDDDHWTTWLDVRGSPLTRHGKLTERFRRP
                     NFGSLQIEVTIDDPKAYTKPFTATINNRLQPDTQLIEFVCLDKSAQHYVGASGVPDAA
                     SKKPTQGR"
     gene            complement(368..793)
                     /locus_tag="AC16CY_13615"
     CDS             complement(368..793)
                     /locus_tag="AC16CY_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354880.1"
                     /translation="MGIDRRAPLACALLLAAGTVAGAHHSFSAEFDSNQPVQLRGTVV
                     RVEWINPHTWIHLDVKKTDGAVERWMIEGGTPNTLLRRGLTRNSLPQGTEVMVDGYKA
                     KNGTNRANGRDITFPDGRKLFMGSSGIGAPRDGKDPNEK"
     gene            complement(819..>1703)
                     /locus_tag="AC16CY_13620"
     CDS             complement(819..>1703)
                     /locus_tag="AC16CY_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354881.1"
                     /translation="DLQGTFSNRTITPFERSPNVKGREFFTPEEVAAMEKAASQSGGD
                     EGRKPGTRGDVDRAYNDFWWDRGTKVTTLRTSMVVDPPDGRVPPQTEEARKRAADEPK
                     RPASRRAGAGGRGTDSWLDRSTFERCITRGLPAAMSPTAYNNNYRITQAPGYVAIEIE
                     MLGGTRVIPTDGRPHVSSSIRQWMGHSTGRWEGDTLVIETTNFTDKVLYRGAAENIHL
                     VERITRLGPNELEYRFTVSDPTTWTRPWTAVIPYVNTGEGIFEYACHEGNYGMEGILS
                     GAREEERKAAEARKSSPQ"
BASE COUNT          240 a          593 c          571 g          299 t
ORIGIN      
        1 cgatgggagg gcgacacgct cgtcgtcgag accagcaact tcatcgacga cgaccactgg
       61 acgacgtggc tggacgtgag aggcagcccg ctgacccgac atggaaagct caccgagcgc
      121 ttccgccggc ccaatttcgg ttcgctccag atcgaggtca ccatcgacga cccgaaggcg
      181 tatacgaagc cgttcacggc gacgatcaac aaccggctgc aacccgacac gcagctgatc
      241 gagttcgtct gcctcgacaa gagcgcgcag cactacgtcg gggcgagcgg cgtgcccgac
      301 gcggcgtcga agaagccgac gcaggggcga tagccgcgat ccgccgtggc gcggcgctgg
      361 tgccgctcta tttctcgttg ggatccttcc cgtcgcgagg cgcgccgatg ccggacgagc
      421 ccatgaacag cttgcggccg tcggggaagg tgatgtcgcg gccgttggcg cggttggtgc
      481 cgttcttcgc cttgtagccg tccaccatca cctcggtccc ctgcgggagc gagttgcgcg
      541 tcagcccgcg ccgcaggagc gtgttgggcg tgccgccctc gatcatccag cgctccacgg
      601 cgccgtcggt cttcttcacg tcgagatgaa tccacgtgtg cgggttgatc cactccaccc
      661 tgacgaccgt gccgcgcagc tgcacgggct ggttggagtc gaactcggcc gagaacgagt
      721 ggtgcgcgcc ggcaaccgtg ccggcggcca gcagcagcgc gcacgcgagc ggcgcacgtc
      781 gatcgatgcc catggtcagt gtccccctct cggcgccgct actgtggcga cgacttcctg
      841 gcctccgcgg ccttccgctc ttcctcgcgc gcgccgctga ggatcccttc catcccgtag
      901 ttgccctcgt ggcacgcgta ctcgaagatc ccctcgccgg tgttcacgta cggaatcacc
      961 gccgtccacg gccgcgtcca cgtcgtcggg tcgctcacgg tgaagcggta ctcgagctcg
     1021 ttcgggccga gacgcgtgat gcgctcgacg aggtgaatgt tctcggccgc gccccggtac
     1081 aacaccttgt cggtgaagtt ggtcgtctcg atgacgagcg tgtcgccttc ccagcggccg
     1141 gtcgagtgac ccatccactg gcggatggag gagctcacgt gcgggcgccc gtcggtcggg
     1201 atgacgcgcg tgccgccgag catctcgatc tcaatcgcga cgtaccccgg agcctgggtg
     1261 atgcggtagt tgttgttgta ggccgtgggg ctcatcgcgg cgggcaggcc acgcgtgatg
     1321 caccgctcga acgtgctgcg gtcgagccag ctgtcggtgc cgcgtccgcc ggcgccggcc
     1381 ctgcgggagg ccggcctctt cggctcgtcg gccgcgcgct tccgcgcctc ttcggtctgc
     1441 ggcggaaccc ggccgtccgg cggatcgacc accatcgacg tccgcagcgt cgtcaccttc
     1501 gtgccgcgat cccaccagaa atcgttgtag gcgcgatcga cgtcgccgcg agtcccgggc
     1561 ttgcggccct cgtcgccgcc gctctggctg gccgccttct ccatcgccgc cacttcctcg
     1621 ggcgtgaaga attcgcggcc cttgacgttg ggtgaacgct cgaacggcgt gatcgtccgg
     1681 ttggagaagg tgccctgcag gtc
//
LOCUS       DBNGSH010000588         1889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_908468, whole genome shotgun sequence.
ACCESSION   DBNGSH010000588 DBNGSH010000000
VERSION     DBNGSH010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_908468"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="AC16CY_13625"
     CDS             <1..443
                     /locus_tag="AC16CY_13625"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR3354882.1"
                     /translation="FARGVFAAIGHPEFEHDPRFATRDARVAHVDEMTAHIETCTRTL
                     STAELLPLLARHNVPAAEVRSPRDAVRDPRVLARGETVALEHPEFGPVADVVGMGIPI
                     TFSDASAGCMRPAPSVGQDNALVYGDWLGYGAAGVEQLQVAGII"
     gene            453..1520
                     /locus_tag="AC16CY_13630"
     CDS             453..1520
                     /locus_tag="AC16CY_13630"
                     /inference="COORDINATES: protein motif:HMM:NF017828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HIR3354883.1"
                     /translation="MHEPCVVASWPSVPVEIVRAAGFRPVMARGASAPTPAADRHLEP
                     RIFPSRLRHLVEDALTGRLAHAARIVIPRTSDADYKCFLYLREFVRRGVATALAPVVL
                     FDLLQSRGADVLAYDVARTRALLDELSSASGRTASADDLRREITRANTARAAARRLIA
                     LRRVVPRVTGTEVFPLLAAFWEMEPDRYVEMASGAANRILARPPLAGPRVLITGAPVD
                     GQTLHEAVESHGAVVVAEVGPWGSGAAGDDVRLDDDPVAALANKYREDSIGARSPVDT
                     LRRWTERMLHDVDAVVVSLPPDDAVFGWDYPALRDVLQARRIPHVCLRGDPYETPTRA
                     DHAALDAMVSAGSRLQEARHG"
     gene            1513..>1889
                     /locus_tag="AC16CY_13635"
     CDS             1513..>1889
                     /locus_tag="AC16CY_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HIR3354884.1"
                     /translation="MADSQLDATLAASAFQKQWFADLRRRVFDDRQPYALLQADVPFE
                     LFDLADIPAVSNQWWAAIVAAKRQAPKFLDAMDADGLHGDLCRYCSLGYASTRYSGAG
                     DPPWGGLPPPRLLCARLTCDCIHR"
BASE COUNT          261 a          661 c          641 g          326 t
ORIGIN      
        1 agttcgcccg cggtgtcttc gccgccatcg ggcaccccga gttcgaacac gacccgcgct
       61 tcgcgacgcg cgatgcccgc gtggcgcacg tcgacgagat gactgcgcac atcgagacgt
      121 gcacgcgcac gctctcgacc gccgaactcc tgccgctgct tgcacgacac aatgtgccgg
      181 cggcagaggt gcgttctcct cgcgacgcgg tccgcgaccc gcgcgtgttg gcgcggggtg
      241 agacggtggc actggagcac ccggagttcg ggcctgtggc tgacgtcgtc ggcatgggca
      301 tcccgatcac cttctcagac gccagcgcag gatgcatgcg tccggcgccg tcggtcggcc
      361 aggacaacgc gctcgtctac ggcgattggc tcggatacgg tgctgccggt gtcgagcagt
      421 tgcaggtcgc gggcatcatc tgagaggcgc gcgtgcacga gccctgcgtc gttgcgtcgt
      481 ggccgtcggt tccagtcgaa atcgtccgcg ccgctggttt ccgcccggtc atggcccgag
      541 gagcttcggc cccgacacca gcggccgaca ggcatctcga gccgcgcatc ttcccgagcc
      601 ggcttcgtca ccttgtcgag gacgctctga caggacgtct cgctcacgcg gcgcgcatcg
      661 tcatcccgcg cacgtcggac gccgactaca agtgctttct ctaccttcgt gagttcgtgc
      721 gcaggggagt ggccacggcg ctcgcgcccg ttgtcctgtt cgacctgctg cagtcgcgcg
      781 gcgcggacgt gctggcctac gacgtggcgc gaacgcgcgc gctgctcgac gagctgtcgt
      841 cggcctcggg ccggacggcc tctgccgacg atctccgcag ggagatcacg cgggcgaata
      901 ccgcccgtgc tgcggccaga cggttgatcg cgcttcgccg cgtcgttcca cgcgtcactg
      961 gaaccgaagt cttcccattg ctcgcggcgt tctgggagat ggaaccggat cgctacgtgg
     1021 agatggcaag cggcgccgcg aacaggatcc tggcgcgccc accgctcgcc ggcccgcgcg
     1081 tgctgatcac tggagcgcct gtcgacggcc agacactgca cgaggccgtc gagtcgcacg
     1141 gcgccgtcgt cgttgccgaa gtcggcccat ggggaagcgg cgccgcggga gacgacgtgc
     1201 gcctcgacga cgacccggtt gccgcgctcg ccaacaagta tcgagaggac tcgatcggtg
     1261 cgcgctcacc cgttgacacg ctgcgccgct ggaccgagcg catgctccac gacgtcgatg
     1321 ccgttgtcgt gtcgctgccc ccggacgacg cggtgttcgg ttgggactat ccggcgctcc
     1381 gcgacgtgct gcaggcgcgt cgcattccac acgtctgtct tcgcggcgat ccgtacgaga
     1441 cgccgacccg tgctgatcac gcggcgctcg acgcgatggt atccgctggc tcgcggctgc
     1501 aggaggcgcg gcatggctga cagtcagctc gatgcgacgc tggccgcgtc agcgttccag
     1561 aagcagtggt ttgccgacct gcgacgccgc gtgttcgacg atcgtcagcc gtatgcgctg
     1621 ctgcaggccg acgtgccgtt cgagctgttc gacctcgcgg atatccccgc ggtcagcaac
     1681 cagtggtggg cggcgatcgt tgccgccaag cgtcaggccc cgaagtttct cgacgccatg
     1741 gatgccgatg gcctccatgg cgatctgtgc cgctactgca gtcttggcta cgcctcgaca
     1801 cgctattcag gcgcaggtga cccgccctgg ggcggactgc cgccgccccg gctgctgtgc
     1861 gcccggctca cgtgcgactg cattcaccg
//
LOCUS       DBNGSH010000589         3038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1926877, whole genome shotgun sequence.
ACCESSION   DBNGSH010000589 DBNGSH010000000
VERSION     DBNGSH010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1926877"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="AC16CY_13640"
     CDS             <1..519
                     /locus_tag="AC16CY_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3354885.1"
                     /translation="LTVAENVGYRLSEESDMPPDEVQRRVVEVLGFIGLGEYIDRMPS
                     ELSGGQRRRVAIARAMASKPSLLLFDDPTTGLDPIIATTVDDEIVKLRDVERVTSIVV
                     THQIRDAFYVATHEAVSANGQLQIRDAGAERARFMVLHEGGIYFEGSGQELLASKDDY
                     LKEFLFMTLPPW"
     gene            complement(785..2293)
                     /locus_tag="AC16CY_13645"
     CDS             complement(785..2293)
                     /locus_tag="AC16CY_13645"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3354886.1"
                     /translation="MRAKVPVLIVLVALAVGAIDAQITSNPLPAPIEKRGVAVEIRDL
                     TRLPDTRSTIPAGQDVNPAGWARVSFVRDLPDGRRFANDSRGFLYLLDSGNTPSVYAN
                     VAAAFPFAVYNRLESGFIGFDFHPEFAKNGLFYTVHGERAAGNPAALNFIPPGYTQKD
                     VTYHNVITEWRATNPAAGTFEGTRRELLRVAHVVMNLTHPFGYVGFNPTAKPGSADYG
                     LLYTSGSDTGFSNGGGPNANNPTQTQRLDSVITAILRIDPRSPSVSKGTKGLGDYTIP
                     PGNKFAADGDPKTLGEIYAYGFRNAHRLSWDQDGTMFATDIGMNHIEEVNIVRNGENY
                     GWMKREGYFENGMIRPGGALDQLFTLPAEVLNGSKKDEFTYPVAIYDHDEGVAVSGGF
                     AYHGRIPALRGKFVFGDVNRGRVFAADLAAMKKADDGIPQTVAPVEEIQLYRRDATGR
                     RTDITFKELVEKTMATTLMRTDLHIGQSRDGELFVTSRQDGWIRVLVPDGGK"
     gene            complement(2307..>3038)
                     /locus_tag="AC16CY_13650"
     CDS             complement(2307..>3038)
                     /locus_tag="AC16CY_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3354887.1"
                     /translation="LAAGVWLLAGGTHNSLLVEFRDYVAVVDAPNNEARSLAVIAEAA
                     RLVPGKPVQYVINTHHHFDHAGGLRTYLSQGTTIVTHESNKQYYLDILFHPWPRTLQP
                     DRMALYNPMYWISRRPPPIETVAGEPRSTAKYVVTDGERILEVIKVQDVAYELGDRSY
                     NQGNHSVDMLIAYLPKEKILFNADLYSPPAQGAAPAAPTPGMRTLQQNMRKLKLDVAR
                     HAPAHGRVGTNDEFMALVASGTKSN"
BASE COUNT          493 a          970 c         1003 g          572 t
ORIGIN      
        1 ctgacggtgg ccgagaacgt cgggtaccgg ctgtcggaag agtccgacat gccccccgat
       61 gaggtccagc gccgcgttgt ggaagtcctg ggcttcatcg ggctcggcga gtacatcgac
      121 cggatgccgt cggagctctc gggcggtcag cgccgccgcg tggcgatcgc ccgcgccatg
      181 gcctcgaaac cgagcctcct gctcttcgac gatccgacga cggggctgga tccgattatc
      241 gccacgaccg tggacgacga gatcgtcaag ctgcgcgacg tcgagcgcgt cacctcgatc
      301 gtcgtcactc accagatccg cgacgccttc tacgtcgcga cgcacgaagc ggtgagcgcg
      361 aacggacagc tgcagattcg cgacgccggc gcagagcgcg ccaggttcat ggtgctgcac
      421 gagggcggga tctacttcga gggaagcggg caggagctgc tggcatcgaa agacgattac
      481 ctgaaggagt tcctgttcat gacattgccg ccttggtaga caggcagcag ttcacacttc
      541 tcgcttctca cttctcggtt cgtgttcagt ttcaggttcg tgttcggggt tcggggttcg
      601 gggttcggtc cggctaaagc cggacactac gtaggctcgg cgaaactctg gcgcgtcctt
      661 ggccggaggc tcgaaacgaa cttggaactc cgaatatgaa ccgcgaactt cgaacacgaa
      721 ccccgaaatt gaacacgaac cgagaagtga gaagcgagaa gtgtgaacga cgggttctct
      781 cgttctactt ccccccgtcg ggtaccagga cacggatcca accgtcctgc ctggaggtga
      841 cgaagagctc accgtcgcgg ctctggccga tgtggagatc cgtgcgcatg agcgtcgtgg
      901 ccatcgtctt ctcgacgagc tccttgaatg tgatgtcggt gcgcctgccg gtcgcatcac
      961 gccgatacag ctgaatctcc tccaccggcg ccacggtctg cgggatcccg tcgtcggcct
     1021 tcttcatcgc ggcaaggtcg gcggcaaaca cgcggcctcg attgacgtcg ccgaatacga
     1081 actttccccg gagcgcgggg atgcgtccgt gataggcgaa cccgcccgac accgccacac
     1141 cttcatcgtg gtcgtagatc gccaccggat acgtgaactc gtccttcttc gacccgttga
     1201 ggacctcggc cggcagcgtg aacagctggt cgagcgcgcc gccaggcctg atcatgccgt
     1261 tctcgaagta cccctcccgt ttcatccagc cgtagttctc cccgttgcgg acgatgttga
     1321 cctcctcgat gtggttcatc ccgatgtcgg tggcgaacat cgttccgtcc tgatcccacg
     1381 acaggcgatg ggcgttgcgg aagccgtagg cgtagatctc gccgagcgtc ttcgggtcgc
     1441 cgtcggcggc gaacttgttg cccggcggaa tcgtgtagtc cccgagccct ttcgtcccct
     1501 tcgacaccga agggctgcgc ggatcgatgc ggaggatcgc ggtgatgacg gaatcgaggc
     1561 gctgcgtctg cgtcgggttg ttggcattcg gcccgccgcc gttgctgaag cccgtatcgc
     1621 tcccgctcgt gtagagcagg ccgtagtcgg cggaccccgg cttcgccgtg gggttgaacc
     1681 cgacgtaccc gaacgggtgc gtgaggttca tcacgacgtg cgcgacgcgg agcagctcgc
     1741 gccgcgtgcc ctcgaacgtg ccagcggccg ggttcgtcgc gcgccactcg gtgatgacgt
     1801 tgtgataggt gacgtccttc tgggtgtatc cgggcgggat gaagttcagc gccgccgggt
     1861 tgccggcggc gcgctcgccg tgcaccgtgt agaacaggcc gttcttcgcg aactccgggt
     1921 gaaagtcgaa tccaatgaag ccgctctcga gcctgttgta gaccgcaaac gggaaggccg
     1981 ccgccacgtt cgcgtagacg gacggcgtgt tgccgctgtc gagcaggtac aggaacccgc
     2041 gcgagtcgtt ggcgaaccgg cggccgtcgg gcaggtctcg cacgaagctc acgcgcgccc
     2101 agccggcggg gttcacgtcc tggccagcgg gaatggtcga gcgcgtgtcg ggcaggcgcg
     2161 tgaggtcgcg gatctcgacc gccacaccgc gcttctcgat cggcgcggga agcgggttgc
     2221 tcgtgatctg cgcgtcgatt gcgccaaccg ccagtgccac caggacgatg agaacaggga
     2281 cttttgcgcg catgagcctg gacctctcag tttgacttcg ttccgctcgc gaccagcgcc
     2341 atgaactcgt cgttggtgcc gacgcggccg tgcgcgggcg cgtgccgcgc gacgtcgagc
     2401 ttcagcttgc gcatattctg ctgaagcgtc cgcatgccgg gcgtcggcgc cgccggagcc
     2461 gccccctgcg cgggcggcga gtacaagtcc gcgttgaaga gaatcttctc cttcggcaga
     2521 tacgcgatca gcatgtccac actgtggttg ccctggttgt acgaccggtc gcccagttcg
     2581 taggcgacgt cctgcacttt gatcacttcc aggatgcgct cgccgtcggt caccacgtat
     2641 ttcgccgtgc tgcgcggctc acccgccacc gtctcgatcg gcggcggccg gcggctgatc
     2701 cagtacatcg ggttgtagag cgccatccga tccggctgca gcgtgcgcgg ccacgggtgg
     2761 aacaggatgt cgaggtagta ctgcttgttc gactcgtgcg tcacgatcgt cgtgccctgg
     2821 gacaggtagg tccgcagccc gccagcatga tcgaaatgat ggtgggtgtt gatgacgtat
     2881 tgaacgggct tgccgggaac gagccgggcg gcttccgcga tcacggccag cgagcgcgcc
     2941 tcgttgttcg gcgcgtccac gacggccaca tagtccctga actcgacgag caggctgttg
     3001 tgcgtccccc cggccaggag ccacacgccc gccgccag
//
LOCUS       DBNGSH010000590         1600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1587663, whole genome shotgun sequence.
ACCESSION   DBNGSH010000590 DBNGSH010000000
VERSION     DBNGSH010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1587663"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1600)
                     /locus_tag="AC16CY_13655"
     CDS             complement(<1..>1600)
                     /locus_tag="AC16CY_13655"
                     /inference="COORDINATES: protein motif:HMM:NF053442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3354888.1"
                     /translation="FVFAGDLWTVPREGGDARRLTTGVGVESNPVYSPDGSTIAFTGE
                     YDGNVDVYVVPAAGGIPKRLTWHPAADAALGWAPDGKRILFSSPRGSYAGFAQLFTTD
                     TNGSFPDKLPLPWGWEAAYSPDAARLAYVPMRRAFTVWKGYRGGDTTPIWLADLSSSK
                     IEKVPHDNTNDFNPMWVGDKVYFLSDRKGPVTLYSYDTRSRRVKEEVENEGLDFKSAG
                     AGPDGIVIEQFGRLLLHDVRSGRTAPVAVTMAGDLAEVRERMVSVRTRLINPDLSPNA
                     ARAVFEARGEIVTVPAEKGDGRVITNTASVMERSPVWSPDGRTIAYFSDETGEYELHL
                     APQAGTGEVTKIKMPEPSFYRSPQWSPDSKYLAFTDARSRMWYLEVTTRKFTQVDKDR
                     YWAAGRGDWAPVWSPDSKWLAYSKRLANYMGAIHVYSLADAKTHQVTDGLSDARDPVF
                     DKDGKYLYFAASTDSGPSLQPDVGSGTRTVTNSIYLAVLAKDQPSPFAPESDEEKVAD
                     PAAADGQKPAADTEKPDTQKPATDT"
BASE COUNT          238 a          542 c          530 g          290 t
ORIGIN      
        1 gtgtatccgt cgccggtttc tgtgtgtccg gcttctctgt gtccgccgcc ggtttctggc
       61 cgtcggccgc ggccggatcg gccacttttt cctcgtcgct ctcaggcgcg aacggcgatg
      121 gctgatcctt ggccaggacc gcgaggtaga tgctgttggt caccgtgcgc gtgccgcttc
      181 ccacgtcggg ctggagcgac ggccccgagt cggtcgaggc ggcgaaatac aggtacttgc
      241 cgtccttgtc gaacacggga tcgcgcgcgt cgctcaaacc atcggtgacc tgatgcgtct
      301 tcgcgtcggc cagcgagtac acgtgaatgg cgcccatgta gttggccagg cgtttcgagt
      361 acgccagcca cttcgagtcg ggcgaccaca ccggagccca gtcccccctc ccggccgccc
      421 agtaccgatc cttgtccacc tgcgtgaact tgcgcgtcgt cacctcgagg taccacatcc
      481 ggctccgggc gtcggtaaag gcgagatact tcgagtccgg cgaccactgc ggcgagcggt
      541 agaagctcgg ctctggcatc ttgatcttcg tcacctcacc ggtgccggcc tgcggcgcca
      601 ggtgcagctc gtactcgccc gtctcgtcgg agaagtaggc gatcgtgcgc ccgtcgggcg
      661 accacacggg tgaccgctcc atgacgctcg cggtgttggt gatgacacgg ccgtcgccct
      721 tctccgccgg aaccgtgacg atctcgccgc gggcctcgaa caccgcgcgg gccgcgttgg
      781 gcgacaggtc cgggttgatg aggcgtgtcc tcacgctcac catccgttcc ctcacctcag
      841 ccaagtcccc cgccatcgtc acggcgaccg gcgcggtccg gccggatcgc acgtcgtgca
      901 ggagcagacg gccaaactgc tcgatgacga tgccgtcggg gcccgcaccg gcggacttga
      961 agtcgaggcc ctcgttctcg acctcctctt tcacgcgacg tgatcgggtg tcgtaggaat
     1021 acagcgtgac cggtcccttg cgatcgctca ggaagtagac cttgtcgccc acccacatcg
     1081 ggttgaagtc gttggtgttg tcgtgaggga ccttttcaat cttcgaagag gagaggtccg
     1141 ccagccagat gggggttgtg tcgccgccgc ggtacccctt ccagacggtg aacgcgcggc
     1201 gcatcggcac gtacgccagc cgcgcggcgt ccggtgaata ggcggcttcc catccccagg
     1261 gcaggggcag cttgtcggga aacgaaccgt tcgtgtcggt ggtgaagagc tgcgcgaagc
     1321 ccgcgtagct gccgcgcggg gagctgaaga ggatgcgctt gccgtcgggc gcccatccga
     1381 gagcggcgtc ggcggccggg tgccacgtga ggcgcttcgg gattccgccc gcggccggga
     1441 cgacatacac gtccacgttg ccgtcgtact cgcccgtgaa cgcgatggtg gagccgtccg
     1501 gtgaatacac cgggttgctc tccacgccca cgcccgtggt caggcgccgg gcatcgcctc
     1561 cctcgcgcgg cacggtccac aagtcgccgg caaagacaaa
//
LOCUS       DBNGSH010000591         3127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3285604, whole genome shotgun sequence.
ACCESSION   DBNGSH010000591 DBNGSH010000000
VERSION     DBNGSH010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3285604"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="AC16CY_13660"
     CDS             <1..704
                     /locus_tag="AC16CY_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR3354889.1"
                     /translation="VGMAGILGNVFSGPGLALWYHFAIMFEAVFILTTIDAGTRVGRF
                     MLQELLGHVSAPLGRTSWYPSILLSSFIVVAGWGYFLYQGVIDPLGGINSLWPLFGIA
                     NQLLAAVALCVATTVLAKMGKLRYTWVTLAPLAWLAAATLTAGWQKVFSPDPKLGFLS
                     HAQSLASAPGPNTARMIFNDRLDAGLALFFMAIVVVVILASAREWYLIASRRKAPAVN
                     EAPFVESRLDVAAGF"
     gene            850..1230
                     /locus_tag="AC16CY_13665"
     CDS             850..1230
                     /locus_tag="AC16CY_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354890.1"
                     /translation="MTGSATDRPLAFVLALGALGGAALITVEWATTKGPLILFPYAAL
                     VIVSAVHLRLERVQGWIRRFSLALGSYTVATLTLYLFIGLVLARTLFDISAWGHMWRL
                     GLMLLIGSALSAAVAQLTATKAAA"
     gene            1499..1996
                     /locus_tag="AC16CY_13670"
     CDS             1499..1996
                     /locus_tag="AC16CY_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354891.1"
                     /translation="MKSKGRHRSLSVMQRGLILAVVVLTAPLWLAVLLVVVLSRVIQV
                     VVLYGLVWVWWIGWARRRVLFVYSDSPNWKDYVEANILPKLPENSVVLNWSHRGSWNK
                     FNVSVMLFQCFAGAQEFNPLGLVFERFNVVERYRFWQAFRDAKHGRLDALQVIERRFL
                     EHAAG"
     gene            2124..2381
                     /locus_tag="AC16CY_13675"
     CDS             2124..2381
                     /locus_tag="AC16CY_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3354892.1"
                     /translation="MITRTRIVQIGNSKGIRVPKMLLEHAQLPDDVELQAEHGRLIVR
                     AARAARAGWSDAAKSMHARGDDRLLDAPTSSQFDDKEWRWR"
     gene            2372..2710
                     /locus_tag="AC16CY_13680"
     CDS             2372..2710
                     /locus_tag="AC16CY_13680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006173278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR3354893.1"
                     /translation="MAVRRDSIGRGEVHLVRLDPTVGSEVRKTRPCLVVSPDELNAHL
                     RTVIVAPMTTGGHAYPWRIPCRFQSRSGFVALDQLRTVDGERLVKRLGKLSAGTTTEV
                     LETLQEMFEE"
     gene            complement(2852..>3127)
                     /locus_tag="AC16CY_13685"
     CDS             complement(2852..>3127)
                     /locus_tag="AC16CY_13685"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR3354894.1"
                     /translation="DDMKENANRESERILREAEGQAEIMMQRAQAQLEDVQREIDGLR
                     LKRRETEVALESIIAALHNTLDFVREQEQREHRVIQHRPMLGVAQSA"
BASE COUNT          489 a          971 c         1030 g          637 t
ORIGIN      
        1 ccgtcggcat ggctggaatc ctcggcaacg tcttcagcgg tccgggcctg gcgctctggt
       61 accacttcgc gatcatgttc gaggcggtct tcatcctcac gacgatcgac gcgggcacgc
      121 gggtcggccg cttcatgctg caggagctgc tgggtcacgt gtcggctccg ctcgggcgca
      181 cgtcgtggta tcccagcatt ctcctctcgt ctttcatcgt cgtcgcgggg tggggctact
      241 tcctgtacca gggagtgatc gatccgctcg gcggcatcaa ctcgctctgg cccctcttcg
      301 ggatcgccaa ccagctcctg gcggccgtgg cgctctgcgt cgcgacgacg gtgctggcga
      361 agatggggaa gctccgctac acctgggtca cgctggcgcc gctcgcatgg ctggcggcgg
      421 cgacgctgac ggcgggctgg cagaaggtgt tctctcccga cccgaagctc gggttcctct
      481 cgcacgcgca atcgctggcg tcggcgccgg ggccgaacac cgcgcgcatg atcttcaacg
      541 atcgcctcga cgcggggctc gcgctgttct tcatggcgat cgtcgttgtc gtgatcctgg
      601 cgtccgcgcg ggagtggtat ctgatcgcgt cgcgacggaa ggcgcctgcc gtgaacgagg
      661 caccgttcgt cgagtcacgt ctggatgtag cggcgggttt ttagaccgga cgtagcggcg
      721 ggtctttcct ggcctgagcg aagtcgaagg cagacccgcc gagtggtcat cgtggccgcg
      781 gcgcggtaca aggtcgctcc gcccgttgtc tttgtagcag cgtgctaaca tccgcgcgga
      841 ggtgctgcga tgactggatc tgcgaccgat cggccgcttg cgttcgtgct cgcgctgggc
      901 gccttgggtg gggcagccct catcacggtt gaatgggcaa ccacgaaagg ccctctgatt
      961 ctcttcccgt atgcagcgct cgtgatcgtt tcggcggtcc atcttcgtct cgagcgcgtc
     1021 cagggttgga tccgtcgctt ctcgctggct ttggggtcgt atacggtcgc gactctcacg
     1081 ctgtatctct tcatcgggct ggtcctggcg cgcacactct tcgacatttc cgcgtggggt
     1141 cacatgtggc gactcgggct catgcttctc atcggctcgg cgctgagcgc ggcagtcgcg
     1201 cagctcaccg ctacgaaagc cgccgcttag ccttgagccc ggcgctttgg cggcagcggg
     1261 gcgctctctt gcccgacgca ccgcggccct tgcaccgggc gagaagcact gcgccgtgga
     1321 aaatgcacta acgacaggcg gccacgccgt ggcgttgcct ccgtgccatc gtgctaccat
     1381 ccccgcgcgg tcaaccgcct tccgcctcgc gaacgaagcg ttctgacctg tattagccgc
     1441 cagggccgat agacaatcgg gcggtccagg caatactggt tagccagaca agacacacat
     1501 gaaatcgaaa ggccgccacc gttcactatc agtgatgcaa cggggcctca ttctggcagt
     1561 cgtagtcctc acggctccgc tgtggctagc ggtgctcctg gtggttgtgc tctcgcgggt
     1621 gatccaagtg gtggttctgt acgggctggt ctgggtgtgg tggataggct gggcgcgaag
     1681 gcgagtgctc ttcgtgtact cagatagccc gaactggaaa gactacgtcg aggcgaatat
     1741 cctgccgaag ctacccgaga acagtgtcgt gttgaattgg tcgcatcgcg ggtcgtggaa
     1801 caagttcaat gtgtcggtca tgttgtttca atgtttcgct ggtgcgcagg agttcaaccc
     1861 gctcgggctc gtgttcgagc gattcaacgt cgtggagcgt tatcgttttt ggcaggcgtt
     1921 ccgggatgca aagcacggac gattggacgc actgcaggtg attgaaaggc ggtttcttga
     1981 acacgcggcc ggctaacagt gcgctgcact cgacaccgcc cgcgtcgctc gcgcgccgca
     2041 accgacgcgg gtgagcgcgg atcgtcagcc cgctggattg cggtccttgc cgaaggcggt
     2101 tgatgtgatt acactgtagt cacatgatca cccgtacccg aatagtccag atcggaaact
     2161 cgaagggcat tcgcgtgccc aaaatgttgc ttgagcacgc gcagctgccg gacgacgtcg
     2221 agctccaggc ggaacatggg cgcttaatcg ttcgcgctgc tcgggccgcg cgggcaggtt
     2281 ggtccgatgc agcaaaatcc atgcacgccc ggggtgacga ccgacttctt gacgcgccga
     2341 catccagcca attcgacgac aaggaatggc ggtggcggta aggcgcgact cgattggacg
     2401 cggcgaagtg cacctcgttc ggctggatcc caccgtgggg agtgaagtcc gcaagacgcg
     2461 gccctgcctg gtagtctcgc cggacgagct gaatgcgcat ctccgaacgg tgattgtggc
     2521 gcccatgacc acgggcggtc acgcctaccc ctggcggatt ccatgccggt ttcagagtcg
     2581 ttcgggtttc gtcgccctgg atcagttgcg gaccgtcgac ggcgaacgtc tcgtaaagcg
     2641 cctgggcaag ctgtccgcag ggacgacgac cgaggtgttg gagacccttc aggagatgtt
     2701 cgaggaatag ctacgcaggc taacgggggc tggacccgac agcggccgcg tggcgatcgc
     2761 gttgcctcgc gccgcgaccg gtgacggcgg gtctgccttc gacttcgctc aggccaggaa
     2821 agacccgccg ctactgaaga ctgaccggga atcaggccga ctgggcaacc cccagcatcg
     2881 gacggtgctg aatgacgcgg tgctcccgct gctcctgctc ccggacgaag tcgagggtgt
     2941 tgtggagcgc ggcgatgatc gactccagcg ccacttcggt ttcgcggcgc ttcagccgca
     3001 acccgtcgat ctcgcgctgc acgtcttcga gctgcgcctg cgcccgctgc atcatgattt
     3061 ccgcctgccc ttcggcctcg cgcaggatgc gctcggactc acggttggcg ttctccttca
     3121 tgtcgtc
//
LOCUS       DBNGSH010000592         4723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1078727, whole genome shotgun sequence.
ACCESSION   DBNGSH010000592 DBNGSH010000000
VERSION     DBNGSH010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1078727"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="AC16CY_13690"
     CDS             complement(<1..557)
                     /locus_tag="AC16CY_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3354895.1"
                     /translation="MNSRSPTPALLLGLVFTLATVVAYSWYISGQISGLRRLQTDLTD
                     RSRRDSLQLLRIQNDLNQLGLAMRDMLDRAEPYPLTAWSSQFQTVRLDLEDALKRQGE
                     VALARQTPEQREYLSTSFSQFWDAVDRIFDLARSGQEGEARAQIQLSLQARQTALSTA
                     VARLLVQNNEAEEQTARQVQEIYVRV"
     gene            complement(649..1107)
                     /locus_tag="AC16CY_13695"
     CDS             complement(649..1107)
                     /locus_tag="AC16CY_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3354896.1"
                     /translation="MTRVTRAIALAACTVMLAATLGSAAQIDSKAVEFTTPDNIKWVR
                     NAAGTNEQAILFGDPEKPGPYVIRLRWLPGNMSRPHYHQNDRFFVVISGTWWLGTGDT
                     FNPDSTVPAPAGSYVIHKAKQIHYDGAKKEPAVIQVWGLGPATSIPAEKK"
     gene            complement(1149..1910)
                     /locus_tag="AC16CY_13700"
     CDS             complement(1149..1910)
                     /locus_tag="AC16CY_13700"
                     /inference="COORDINATES: protein motif:HMM:NF028121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HIR3354897.1"
                     /translation="MGIGIFVLTALLIAAAAPARQAAKDPDRLYADRENLTSALEAAS
                     IWEARLSKDKQDFEAAWKLARASYWLGGHVAEGDRRKQYERGIEAATHAVALQPKRPE
                     GHFWMAADMGAMAEFFGLRAGIRYRGPVKRALETVLQIDPAFQQGSADRALGRWYFRV
                     PRLLGGSRDKAIEHLKRSLTYAPRSTATRYFLAETYFEMDRRDEARREAQQVLDAPLD
                     PDWAPEDREFKQQAKSLLQKMGSGVIIFQDASPKK"
     gene            complement(1986..2933)
                     /locus_tag="AC16CY_13705"
     CDS             complement(1986..2933)
                     /locus_tag="AC16CY_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354898.1"
                     /translation="MTRPSCVLVVVLMLGAPAGAQTPRTPWGDPDLQGTFTTDNSIGV
                     PLERPKELGTRTTLTDDEFAAREKANAAQVALDQGDLPESKVKKDDAPNNAPRHWLER
                     PARPSRATSLVIDPPDGRIPALTAEAQQLASARRAQRGRGLPASTADFSNYDRCISRG
                     VVGSILPAIYGNGTSILQAPGYVVIRNEMIHEARVIPLDGRPHVSSDIAMWMGDARGR
                     WDGPTLVVDSTSFTDRTGIGGGANHTRRLHLVERFTRVDADTVRYEVTVDDAATYTRP
                     WTIRVDLDQKKGYEIYEYACHEGNYGLRNMLSAARAEEK"
     gene            complement(2941..3798)
                     /locus_tag="AC16CY_13710"
     CDS             complement(2941..3798)
                     /locus_tag="AC16CY_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3354899.1"
                     /translation="MLTSSIDVDRLTAIDVHVHLEHTGDETEADKHAAAYFKGGAARD
                     AASLAEYYRSRKMACVIFTVDETLTGMPRLSNDEVVAFAQSNADIAIPFASINPTRGA
                     DAVREARRLVAAGAVRGLKLHPPIQEFFPNDRIAYPLYEVFAEARLPVVFHTGHSGIG
                     TGMRGGGGIRLKYGAPMPIDDVAVDFPDMPIIMAHPSFPWQDEAISICLHKPQVYIDL
                     SGWSPKYFSPTLVQYSNTLLKQKVMFGSDYPLLTPDRWLADFEKLPIRDEVRPLILKE
                     NAVRLLGLR"
     gene            complement(3798..>4723)
                     /locus_tag="AC16CY_13715"
     CDS             complement(3798..>4723)
                     /locus_tag="AC16CY_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR3354900.1"
                     /translation="PAAIIATNTSTLDIDDIASSTGRPPKVLGLHFFSPANVMRLVEI
                     VRGKHTSPGTIATALAVAKRLGKVGVVVGNCPGFVGNRMMFPYMYEAQFLVEDGATPQ
                     QVDEALTGFGMAMGIFAVDDMAGLDIAWRVRQELRQFSEPGARKPFAADALCEAGRFG
                     QKTGKGWYVYGEDRKPIPDPEVHALVERLSAEHGITRRPVTPVEIVERTIYALINEGA
                     RTLEEGFALRAADIDVIYMTGYGFPAWRGGPMFYADRVGLKKVYDRVSDFHREHGQRW
                     APAPLLARLAREGGTFKQWDQSRSGAALQAR"
BASE COUNT          760 a         1557 c         1535 g          871 t
ORIGIN      
        1 acccgcacgt agatctcctg gacctgccgg gccgtctgct cctcggcctc gttgttctga
       61 acgaggaggc gcgccacggc cgtgctgagc gccgtctgcc gtgcctggag cgacagttga
      121 atctgtgcgc gcgcctcgcc ctcctgcccg gagcgcgcga ggtcgaagat ccggtcaacc
      181 gcgtcccaga actgactgaa cgaggtcgag aggtactcgc gctgctcggg cgtctgcctg
      241 gcgagtgcca cttcgccctg gcgcttcagg gcatcctcga gatcgagtcg tacggtctga
      301 aactggctcg accaggcggt cagcggatag ggctcggcgc ggtccagcat gtcgcgcatg
      361 gcgagaccga gctggttgag gtcgttctgg attcggagca gttgcagcga gtcgcggcgg
      421 cttcgatcag tgagatcggt ttgcagccgc cgaagcccgg atatctggcc ggagatgtac
      481 cacgagtagg ccaccacggt cgcgagcgtg aagacgagcc ccagcagcag ggcgggagtg
      541 ggggagcggg agttcaactc ccaactccca agccgatcct cgttccaact cccaacccga
      601 tccctgtccc attgggagtt gggaattggg agttgggagt tgagtgcctc acttcttctc
      661 ggcgggaatg gacgtggccg gccccagtcc ccagacctgg atcacggcgg gctccttctt
      721 ggccccgtcg taatggatct gcttcgcctt gtggatcacg taggaaccag ccggcgcggg
      781 gactgtgctg tcgggattga acgtatctcc cgtgccaagc caccacgtgc ccgagatcac
      841 gacgaagaac cgatcgttct ggtggtagtg cggccggctc atgttgcccg gcagccacct
      901 gagacggatc acatacggcc ccggcttttc cgggtcgccg aaaagtatgg cctgttcgtt
      961 ggtgcctgcc gcattcctga cccacttgat gttgtcgggc gtggtgaact ccaccgcctt
     1021 gctgtcaatc tgcgcggcgg aaccgagcgt ggcggcgagc atcaccgtgc aggctgccag
     1081 cgcgatcgcg cgtgtcactc gagtcatggc tcctccctcg agggcgtacg atagcacggg
     1141 gtcgggagtc attttttggg ggatgcgtcc tgaaaaataa tgactcccga ccccattttc
     1201 tgcagcagcg acttcgcctg ctgcttgaac tcccgatcct cgggcgccca gtcgggatcc
     1261 agcggcgcgt cgagcacctg ctgggcctcc cgccgcgcct cgtcgcgtcg atccatttcg
     1321 aaatacgtct cggccaggaa gtagcgcgtt gccgtgctcc gcggcgcgta cgtgagcgac
     1381 cgcttcaagt gctcgatggc cttgtcccgg ctgccgccga gcagacgcgg cacgcggaag
     1441 taccaccgcc cgagcgcgcg gtcggccgag ccctgctgga aggccgggtc gatctgcagc
     1501 accgtttcga gcgcccgctt gaccggcccg cggtaccgga ttccggcccg caggccgaag
     1561 aactcggcca tggcgcccat gtcggccgcc atccagaagt ggccttcagg acgcttgggc
     1621 tgcagcgcca ccgcgtgcgt cgccgcctcg atgccgcgct cgtactgttt tcgccggtcg
     1681 ccttcggcga cgtggccgcc cagccagtag gacgcgcgag ccagcttcca ggcggcctcg
     1741 aagtcctgct tgtccttgga cagacgagcc tcccagatgg acgccgcttc cagcgcgctc
     1801 gtgagattct cgcggtcggc gtacaggcgg tcagggtctt ttgcggcctg tcgcgcgggc
     1861 gctgcagcgg cgatcagaag cgctgtcagg acgaagatcc caattcccaa cttccaactc
     1921 ccaacttcca acccccaact cctaactcca gcatcctttg ggagttgggc attgggagtt
     1981 gggagttact tctcttccgc ccgtgcggcg ctcagcatgt tccgcaggcc gtaattccct
     2041 tcgtggcagg cgtactcgta gatctcgtat cccttcttct gatccagatc gacgcgaatg
     2101 gtccagggtc gcgtatacgt cgcggcgtcg tccacggtga cttcgtaccg aaccgtgtcg
     2161 gcgtcgacgc gggtgaatcg ctcgacgaga tgcagccgtc gggtgtgatt ggcaccgccg
     2221 ccgatccccg tacgatcggt aaagctggtc gagtcgacga cgagcgtggg tccatcccat
     2281 cgcccgcgcg catcacccat ccacatcgca atgtcgctgg agacgtgcgg ccggccgtcg
     2341 agagggatca cgcgggcctc gtggatcatc tcgtttcgaa tcacgacgta tccaggtgcc
     2401 tggaggatgc tcgtgccgtt gccgtagatc gccggcagga tggagccgac cactccgcgg
     2461 ctgatgcagc gatcgtagtt gctgaaatca gccgtcgatg ccgggagccc ccggccccgc
     2521 tgcgcccgcc gggccgaggc gagctgctgg gcctcggcgg tcaacgccgg gatgcgcccg
     2581 tcaggcggat cgatgacgag cgacgtggcg cgagaaggac gggccggccg ctccagccag
     2641 tgccgcggcg cattgttggg ggcatcgtcc ttcttgacct tgctttccgg gagatctccc
     2701 tggtcgaggg cgacctgagc ggcgttggcc ttctcgcgcg cggcgaactc gtcgtcggtc
     2761 aacgtcgttc gagtgcccaa ctccttcgga cgctcgagcg gcactccgat ggagttgtcg
     2821 gtcgtgaacg tgccctgaag atccggatcg ccccagggcg tgcgcggcgt ttgtgcgcct
     2881 gcgggggcgc ccagcatcaa aacgaccacg aggacgcacg atggtcgagt catgggcgtg
     2941 ctacctcaat ccgagcagtc gaacggcatt ctccttcagg atcaacggcc ggacctcgtc
     3001 acgaatcggc agcttctcga agtccgccag ccatcgatcc ggcgtcagca gcggatagtc
     3061 cgatccgaac atcaccttct gcttcagaag cgtattggag tactgcacga gcgtcggcga
     3121 gaagtacttc ggcgaccatc cggacaagtc gatgtagacc tgcggcttgt gcaggcagat
     3181 cgagatggcc tcgtcctgcc acggaaacga ggggtgcgcc atgatgatcg gcatgtccgg
     3241 gaaatccacg gccacgtcgt cgatcggcat cggcgcgccg tacttcagcc ggatgccgcc
     3301 cccgccgcgc atgccggttc cgatgccgct gtgcccggtg tgaaacacga ccggcagccg
     3361 tgcttccgcg aagacttcgt acagcggata ggcaatccgg tcgttgggga agaactcctg
     3421 aatcggcggg tgcagcttga ggccgcgcac tgctccggcc gccacgagac gacgggcctc
     3481 gcgcacggcg tcggcaccgc gcgtggggtt gatgctggca aacggaatcg cgatgtcggc
     3541 gttgctctgc gcgaaggcga cgacctcgtc gttcgagagc cggggcatcc cggtgagcgt
     3601 ctcgtccacg gtgaagatca cgcacgccat cttgcgcgaa cggtagtact cggcgagcga
     3661 cgcggcgtca cgagccgcgc ctcccttgaa gtacgccgct gcgtgcttgt cggcctcggt
     3721 ttcgtcgccg gtgtgctcga gatgaacgtg aacgtcgatg gcggtcagcc gatcgacgtc
     3781 gattgaagag gtcagcatca gcgcgcctgc agtgccgcgc ccgagcggct ctgatcccac
     3841 tgcttgaagg ttccgccttc gcgtgccaac cgcgcgagca gcggcgccgg cgcccatcgc
     3901 tggccgtgct cgcgatggaa gtcggaaacg cggtcgtaca ccttcttcag tcccacgcga
     3961 tccgcgtaga acatcggacc gccgcgccag gccgggaacc cgtatccggt catgtagatc
     4021 acgtcgatat ccgcggcacg aagagcgaaa ccctcctcca gcgtccgcgc gccttcgttg
     4081 atgagtgcgt agatcgtacg ctcgacgatt tccacgggcg tcacgggccg ccgtgtgatg
     4141 ccgtgttccg cagagagccg ctcgacgagt gcgtgcacct ccgggtcggg gatcggcttg
     4201 cgatcctcgc cgtacacgta ccaccccttc ccggtcttct ggccgaatcg gcccgcctcg
     4261 cagagcgcgt cggcggcaaa cggcttcctc gctcccggct cgctgaactg ccgcagctcc
     4321 tgacgaacgc gccacgcgat gtcgagcccc gccatgtcgt ccacggcgaa gatccccatc
     4381 gccatgccga agccggtcag cgcctcgtcc acttgctgcg gcgtggctcc atcttcgacg
     4441 aggaattgcg cttcgtacat gtaggggaac atcatccggt tgccgacgaa gcccggacag
     4501 ttacccacca ccacgccgac cttgcccagc cgtttggcca cggcgagcgc tgtcgcgatc
     4561 gtccccgggc tggtgtgctt tccgcggacg atctccacga gccgcatgac gttggcaggg
     4621 ctgaagaagt gcagacccag gaccttgggc gggcggcccg ttgatgacgc tatgtcgtcg
     4681 atatcgagcg tcgaggtatt ggtggcgatg atggccgccg gct
//
LOCUS       DBNGSH010000593         6491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_908743, whole genome shotgun sequence.
ACCESSION   DBNGSH010000593 DBNGSH010000000
VERSION     DBNGSH010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_908743"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="AC16CY_13720"
     CDS             <1..608
                     /locus_tag="AC16CY_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354901.1"
                     /translation="TFLACLAGHATTLSAQGVEVTPFGGYRFGGDFFELLTGQPLDID
                     GAPSVGVIVDVPTGGGYFVEGLFTHQSANVFVPTIPLGAPVQWRLTVDHWQAGGLQEF
                     DIRRPRARPFLTGALGLTRYAGDRDDNEFRFTLSAGGGIKLLPSRNIGVRLSGNLFAT
                     FVVADALFFACGGGTGTCLIGFHANIVWQIEFTAGLIVRFD"
     gene            689..1960
                     /locus_tag="AC16CY_13725"
     CDS             689..1960
                     /locus_tag="AC16CY_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR3354902.1"
                     /translation="MSRIARSLVFLILVLVLEPARAGAQAPQSAPPDAPQAREPGPQE
                     ETIDIFDVWRQVRNKEPDPPEEAADYRKPMVALAPVIGVKPSAGVSFGVAGNIAFYRG
                     DPSTTRISSVVTSATFSTEKQTSIKERFTLFTRDDRWRFEGDHRFEWTSLDTYSLGMD
                     AEVGAGIEARFDFFRAYQTAYVRVARSLFAGGGLYFDNHTNVGPAAGAEEAWASSPYV
                     EYSETYGLPIDSQISAGPSLDLLWDDRDNFINADRGWLVKASYRKSFEGFLGGDSTWE
                     KLTVDARTYVPLTPDLRHKLAFWAYTDHVVGGVAPYLDLPASGQDAYGRSARGYHEGQ
                     FRGPKLAYGEVEYRGTLMRNGLLGMVLFLNATTVTTPDGSERLFDSVAIGGGAGVRLL
                     INKRSKTNLCFDVGFGERGSSGVYVGVQEVF"
     gene            complement(2323..3186)
                     /locus_tag="AC16CY_13730"
     CDS             complement(2323..3186)
                     /locus_tag="AC16CY_13730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3354903.1"
                     /translation="MRMLAPCAAMIVVLGGLSTDAQKPTSASGLRFEVASVRPNESGD
                     PHGRSAVYPGGRYMAVNVTLEELTVQAYGLQPSQLADGPGWMRAERFDVTARADGEFA
                     ATGAVEGAGSERLQAMLQALLADRFKFVAHRETREQPVYALVPARDDKALGPKMRVST
                     TDCDAPPAGPGRAGEPSPEPTGLTTPVPCTLRMGHGVLVARDATLGELAVSLSRTVQR
                     VVHDQTGLVERYDMDMTFTPERMPRVRPADPDETSIFTALREQLGLKLESTRAPVEVI
                     VIDTVERPTPD"
     gene            complement(3587..5251)
                     /locus_tag="AC16CY_13735"
     CDS             complement(3587..5251)
                     /locus_tag="AC16CY_13735"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3354904.1"
                     /translation="MRAVTIPRFIPGVALAAALVCTPLFAQPQPAPAAQAPPSLAAVA
                     VRDAIPSGEAFTLTYFNRPIVVLRARVLGRGPAERARIAVGVLDGLVAERTTSPIESR
                     TIEGGSLIFVGNRVIFGLAPPDIDELAGETLEGVTAQALMRLQQAFDEAGEAYTAAAL
                     VRAAIAAAIALVIGLLVLWGLGRARRGVAAKLAAISERTAARAGLDDPVALRASRLFE
                     FERGLVNAVTAALQLVVVYGILSFVLGQFPYTRPWGESLSGFLWTTLKDLLLGAAYAI
                     PGLFTVVVIFLLARLLVRLVASWFRSVERGRITPAWIHPETAQPTRRILTTLVWVLAI
                     VVAYPYMPGSETEAFKGVSVFVGLMLTLGSSGLVNQIVSGFTVTYSRAMRVGDFVKIG
                     DVEGTVTHLGLLSTKLKTLQLEEVTIPNALVASQTATDYSRYAEAEGVFTPTSVTIGY
                     DTPWRQVHTLLLLAAERTPGLRQEPKPRVYQTSLEDFYVKYTLWVSLERQESRLVTFD
                     RLHANIQDLFNEYGVQIMSPNYLADPAAPKVVPKKDWFAAPAKRDE"
     gene            complement(5248..6048)
                     /gene="ppk2"
                     /locus_tag="AC16CY_13740"
     CDS             complement(5248..6048)
                     /gene="ppk2"
                     /locus_tag="AC16CY_13740"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HIR3354905.1"
                     /translation="MAKDRKNGKLKTKDYEKELTKLQGELCVLQEWVKAKGLRVIIVF
                     EGRDAAGKGGTIKAITERVSPRVFRLVALPAPSDREKSQMYMQRYMQYFPAAGEIVIF
                     DRSWYNRAGVEHVMGFCTQEQYERFLELAPEIEKYITEGGIILIKFWLEVSNKEQARR
                     FEARIADPLRQWKLSPMDLPSRAKWYEYSRARDAMLKATDTKHAPWYIVRSDDKKRAR
                     LNCIAQLLKLIPYKKVDRPKVKLPNRSKKHAYDDEATIVKRRFVPEIY"
     gene            complement(6145..>6491)
                     /locus_tag="AC16CY_13745"
     CDS             complement(6145..>6491)
                     /locus_tag="AC16CY_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3354906.1"
                     /translation="RLRRNAEAAQARFDAALAGYQKAALNGYREVANALVTIQKLAEE
                     RVQHQAGVTALLDAADLARARYDSGLASYIEILTADQDLFEQQLLLAQTRGAEFRARA
                     ELYRALGGGWQP"
BASE COUNT         1048 a         2154 c         2151 g         1138 t
ORIGIN      
        1 tgacgtttct ggcgtgtctg gccggccacg ccacgacgct ctcggcgcaa ggggtcgaag
       61 tcacgccctt tggcgggtat cgattcgggg gcgacttctt cgagttgctc acgggtcagc
      121 cgctcgacat cgacggtgcg ccctcggtgg gcgtgatcgt ggacgtgccg accggcggcg
      181 gctacttcgt cgaagggctg ttcactcatc agagcgccaa cgtcttcgtg ccgaccatac
      241 ccctgggcgc gcccgtgcaa tggcggctca ccgtggatca ctggcaggcc ggcggactgc
      301 aggagttcga catccgacgg ccccgtgccc ggccctttct caccggcgcc cttggcttga
      361 caaggtacgc cggcgacagg gacgacaatg agttccgctt cacgctcagc gccggcggag
      421 gcatcaagct gcttccctcg cggaacatcg gcgtgcggct cagcggcaac ctctttgcga
      481 ccttcgtcgt ggccgacgcg ctgttcttcg cgtgcggcgg gggcacgggc acctgtctca
      541 tcggcttcca cgccaacatc gtctggcaga tcgagttcac cgcggggctc atcgtcaggt
      601 tcgactagag cagggggcag ggagcagtgg gcaggggtca gggctgtcca aggcatcagg
      661 ccttgcaggt gagagaatcg agccaggcat gtcacggatc gcccgcagcc tggtctttct
      721 catcctcgtc ctcgtcctcg agcctgctcg cgccggcgcg caggcgccgc agagcgcgcc
      781 tcccgacgcc ccgcaggcac gtgagccggg cccgcaagag gaaacgatcg acatcttcga
      841 cgtgtggcgg caggtccgga acaaggaacc ggatccgccc gaggaggccg cggactatcg
      901 caagccgatg gtggccctgg cgccggtcat cggtgtgaag ccgtcggccg gagtgtcctt
      961 cggcgtggca ggcaacatcg cgttctaccg aggcgacccc tcgactaccc ggatctcgtc
     1021 cgtcgtgacg agcgccacgt tctcgaccga gaagcagacc tcgatcaagg agcgcttcac
     1081 gctcttcacc cgtgatgacc gatggcgctt cgagggcgac caccggttcg agtggacgtc
     1141 gctcgacacc tacagcctgg gcatggacgc cgaggtgggt gccggcatcg aagcgcgttt
     1201 cgatttcttt cgcgcgtatc agacggccta cgtgcgcgtg gcgcgctcgc tcttcgcggg
     1261 cggaggcctc tacttcgaca accacaccaa tgtcgggccg gccgccggcg cggaagaggc
     1321 gtgggcctcc tcgccgtacg tcgagtacag cgaaacctac ggccttccga tcgactcgca
     1381 gatctcggca ggcccgagcc tggacctgct ttgggacgat cgcgacaact tcatcaacgc
     1441 cgatcgggga tggctcgtga aggcgagcta ccggaagtcg ttcgagggct tcctcggggg
     1501 cgattcgacc tgggagaaac tcaccgtgga cgcgcggacg tacgtaccgc tcacgccgga
     1561 cctgcgtcac aagctcgcct tctgggcgta taccgaccac gtcgtcgggg gtgtcgcgcc
     1621 gtacctggac ctgcccgcaa gcggtcagga cgcctacggg cgctcggcgc gcggatatca
     1681 cgaggggcag ttccggggcc cgaagctcgc gtacggcgag gtcgagtacc gcgggacgct
     1741 gatgcgcaac ggactgctcg gcatggtcct gttcctgaac gccacgacgg tgacgactcc
     1801 ggacggcagc gagcgcctct tcgatagtgt ggcgataggg ggcggcgcgg gagtccggct
     1861 gctgatcaac aagcgatcga agacgaatct ctgcttcgac gtcggattcg gcgaacgggg
     1921 ctcgagcggc gtctacgtcg gcgtccagga agtgttctga ggtccgcgtc ccaagtcccg
     1981 aatcaacggg gtgcttcagt gtccggcttc agcccgaccc tgatcctcgt ggtatccggc
     2041 ttcagcaaga atctctcaag ggacaaggca aatgcgacta tcaagagatg atgtcgtcaa
     2101 ctacatccga aagttcttgg aaggttcggg cgagcagtgg gattgggatg atttcatgtc
     2161 aatccctctg gacgatccta ctcttgaacg agtgcggctc gagtgcgtga gcctgccaga
     2221 tcgctttccc ccaagacaga agggtcagta ttgcagcgac gccgggctcg cagaacttcg
     2281 caggttggcc cagacgctcg aatcgtcatg attgcggtgc gatcagtcag gggtcgggcg
     2341 ctcgacggtg tcgatgacga tgacctccac cggggcgcgc gtggattcca gcttcagccc
     2401 aagctgctcc cggagggcgg tgaagatcga cgtctcgtcc gggtcggctg gtcgcacccg
     2461 cggcatccgc tcgggagtga acgtcatgtc catgtcgtag cgctcgacga gtccggtctg
     2521 atcgtggacc actcgctgga ccgtgcgcga gagactgact gccagctctc cgagggtcgc
     2581 atcccgcgcc acgagcacgc cgtgtcccat gcggagcgtg cacggcactg gcgtcgtgag
     2641 gcccgtaggc tccggcgacg gctcgcctgc tcgtcccggc ccggcaggcg gggcatcgca
     2701 gtcagtcgtg gacacgcgca tcttgggccc gagggccttg tcatcgcgtg caggcacgag
     2761 cgcatacact ggctgctccc tggtctcgcg gtgtgcgacg aacttgaagc gatcggccag
     2821 gagcgcctgg agcatcgcct ggagccgctc ggaaccggcg ccctcgaccg cgcccgtggc
     2881 ggcaaactcg ccgtcggctc tggcggtgac gtcgaaacgc tcggcacgca tccagccggg
     2941 cccgtccgcg agctggctcg gctgcaagcc gtacgcctgc acggtgagct cttcgagcgt
     3001 cacgttcacg gccatgtagc gtccgcccgg gtacacggcg gagcggccgt gcgggtcacc
     3061 tgactcgttc ggcctgaccg aggcgacctc gaagcgtagg cccgaggccg aggtcggctt
     3121 ctgtgcgtcg gtgctgagcc caccgagaac gacgatcatc gcagcgcatg gcgcgagcat
     3181 ccgcatggct gtgcctccca gatgactccg gaccagtccc gggcctcccg agcggacttc
     3241 gtgtcccgag ctcaggaccc gtggtgccgc gattctaccg ccagttcccg cccagctcta
     3301 ctcgatgtgc acgatccctc gccgcagcgc gacgggccgg cgtgcgaaga cggtcagcgg
     3361 ggcgagcacg gggttccgct acgacggccc tcaacaccgg gccagcagct tccggatagt
     3421 actttgtccg agacatactc catcctgctc cacgtcatgt gaggcacacc tcgttggtcg
     3481 gcggcgaagc tgaggtgctt ggtcgaaagc cggttttgcg gagcggccca cggtccccgc
     3541 actgcgtcca ctctgacaga cagcgcttgg cgcggcagtc actggactac tcatcacgtt
     3601 tcgccggcgc tgcgaaccag tccttcttcg gcactacctt cggcgcggcg ggatcggcca
     3661 ggtaattcgg cgacatgatc tggacgccgt actcgttgaa cagatcctgg atgttcgcgt
     3721 gcagcctgtc gaaggtgacc agcctcgact cctgccgctc gagcgacacc cacagcgtgt
     3781 acttcacgta gaagtcctcg agcgaggtct ggtagacgcg cggcttcggc tcctggcgaa
     3841 ggccgggcgt ccgctcggcc gcgagcagca ggagcgtgtg tacctgccgc cacggcgtgt
     3901 cgtagccgat ggtcaccgac gtcggcgtga acacgccctc ggcctcggcg taccgcgagt
     3961 agtcggtggc cgtctgggac gcgacgaggg cgttggggat cgtgacttcc tcgagttgca
     4021 gcgtcttgag cttcgtggac agcaggccga gatgagtcac cgtgccctcg acgtctccga
     4081 tcttcacgaa gtcgccgacc cgcatggctc gcgaataggt gaccgtgaag ccgctcacga
     4141 tctggttcac gaggccgctc gagccgagcg tcagcatcag gccgacgaag acgctgacgc
     4201 ccttgaacgc ctccgtctcg cttcccggca tgtagggata cgccacgacg atcgccagca
     4261 cccacaccag cgtcgtcagg atccgccggg tcggctgggc ggtttcggga tggatccagg
     4321 ccggcgtgat ccgtccccgt tcgaccgagc ggaaccatga cgcgacgaga cggaccagca
     4381 gccgcgccag caggaagatc accacgacgg tgaacagtcc cgggatggca tacgccgcgc
     4441 cgaggaggag gtccttcaac gtcgtccaca ggaatccgct gagcgattcg ccccacgggc
     4501 gggtgtacgg gaactgcccg aggacgaagc tgaggatgcc gtagaccacg acgagctgta
     4561 aggcggcggt cacggcgttg acgagcccgc gctcgaactc gaacaggcgc gatgctcgaa
     4621 gggcgacggg atcgtcgagc ccggcccgcg ccgcggtcct ttccgagatg gcggccagtt
     4681 tcgccgccac gcctcgtctg gcacggccga ggccccagag caccagcagg ccgataacca
     4741 gggcgatggc ggcggcgatg gcggcgcgga ccagcgcggc ggctgtatac gcttcgcccg
     4801 cctcgtcgaa tgcctgctgc agtcgcatca aggcctgggc ggtgacgccc tcgagggtct
     4861 cgccggcgag ttcgtcgatg tcgggcgggg ccagcccgaa aatgacccgg ttaccaacga
     4921 agatgagcga gccgccctcg atcgtgcgcg attcgatcgg cgacgtcgtc cgctcggcca
     4981 ccaggccgtc gagcacgccg acggcaatcc tcgcgcgctc ggccggaccg cgccccagga
     5041 cccgcgcgcg caggaccacg atggggcggt tgaagtacgt cagcgtgaac gcctcccccg
     5101 agggaatcgc gtcgcgcacg gcgacggccg ccagcgacgg cggcgcctgg gccgcaggcg
     5161 caggctgcgg ctgcgcgaag aggggagtgc agacgagcgc cgccgcaagc gcgacgcccg
     5221 ggatgaagcg cgggattgta actgctctca atagatctcc ggcacgaacc tgcgcttgac
     5281 gatcgtcgcc tcgtcgtcgt aggcgtgctt cttcgaccgg ttgggcagct tcaccttcgg
     5341 tctgtcgacc ttcttgtaag ggatgagctt caacagctgc gcgatgcagt tgaggcgcgc
     5401 gcgcttcttg tcgtcagacc tgacgatgta ccacggcgcg tgcttggtgt cggtcgcctt
     5461 gagcatcgcg tctcgcgccc gcgagtactc gtaccacttg gcgcgcgacg gcaggtccat
     5521 cggactcagc ttccattgcc gaagggggtc ggcgatacgc gcctcgaacc ggcgcgcctg
     5581 ctccttattg ctgacttcga gccagaactt gatgaggatg atgccgcctt cggtgatgta
     5641 cttctcgatc tccggcgcca gttcgaggaa ccgctcgtac tgttcctgcg tgcagaagcc
     5701 catgacgtgc tcgacgccgg cgcggttgta ccagctgcgg tcgaagatca cgatctcgcc
     5761 ggcggccgga aagtactgca tgtaccgctg catgtacatc tggctcttct cgcggtccga
     5821 cggcgccggc agcgccacga gccggaagac gcgggggctg acgcgctcgg tgatcgcctt
     5881 gatcgtcccg ccctttcccg ccgcgtcccg cccttcgaag acgatgatta cccggagccc
     5941 cttggctttg acccattcct gcagcacgca caactcgccc tgcagcttgg tgagctcctt
     6001 ttcgtagtcc ttcgtcttca gcttcccgtt cttcctgtct ttggccatgc ggttcctttc
     6061 ctaactccca actcccgatt cccgacttcc aaggcccaat tcattgagag tgtgggcgtt
     6121 gggcgttggg atttgggagt tatgtcaagg ctgccagccc ccgccgagcg cccggtacag
     6181 ctccgcgcgc gctctgaact ccgcgccacg ggtctgcgcg agcagcaact gctgctcgaa
     6241 gaggtcctga tcggcggtga gaatctcgat gtagctggcg agtccggaat cgtagcgcgc
     6301 ccgcgcgaga tcggccgcgt cgagcagcgc cgtcaccccg gcctggtgct gtacccgctc
     6361 ttcggccagc ttctgaatcg tcaccagcgc gttggcgacc tcgcgatagc cgttgagcgc
     6421 cgccttctgg tagcccgcca gcgcggcgtc gaatcgagcc tgcgccgcct cggcgttcct
     6481 ccgcaggcgt c
//
LOCUS       DBNGSH010000594         2959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2776533, whole genome shotgun sequence.
ACCESSION   DBNGSH010000594 DBNGSH010000000
VERSION     DBNGSH010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2776533"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..808)
                     /locus_tag="AC16CY_13750"
     CDS             complement(359..808)
                     /locus_tag="AC16CY_13750"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3354907.1"
                     /translation="MSVRTLLLALCLSLAAFDAALAQSVKSDQEILIDLERQWDAAFH
                     SGDATFIETILADEFIATYGDGSRGDKAKELALAREQNQQIDSRRLDDFIIKVYRDTA
                     VVWFTQYLVGPVQGKPTEVQYQYMDVWVVRDGRWKCVASQSTRVGAR"
     gene            complement(853..1575)
                     /locus_tag="AC16CY_13755"
     CDS             complement(853..1575)
                     /locus_tag="AC16CY_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR3354908.1"
                     /translation="MFLLGVFALAVLSGGTAAVAGFGIGSLLTPLLAARFDTGTAVAA
                     VAIPHAAATALRCWRLRKAVDWTVLRRFGLLSAAGSLAGALLYTRLSNASLTLTLAVL
                     LLATSAATLGDLPSKVRVTGPVAGVLGLLSGVFGGLAGNQGGLRAAAMLSFSLSPGPY
                     VATATATAMMVDLVRTPIYLCCAGGDLYTLGVPIAVATAGVLVGTLTGERILLGLGPR
                     QFRRVIAAIVGLLGVWLIVQAG"
     gene            complement(1577..2335)
                     /locus_tag="AC16CY_13760"
     CDS             complement(1577..2335)
                     /locus_tag="AC16CY_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354909.1"
                     /translation="MRTFLQLAVVALVAAPAFAQWTKIPPPQVPLTPDGKPNLSAPAP
                     RLPDGKPDLSGIWNPPTGYLRNLARDLKEEVPFQPWAKAVYDERAAGIHWKSEPDANC
                     LPQGVPKVLLAPAPWRIVQTPRVVYFIHEAFNLWWQAFMDGRQFVPHPDVQPTWHGYS
                     TAKWEGDTLVVDSQGFNGRVWLDQLGKPSTEALHVTTRFRRKDFGHMEIGITIDDPKA
                     YTKPWGATVEVTLLPNTELMEFICLENEKDTRPK"
     gene            complement(2349..2867)
                     /locus_tag="AC16CY_13765"
     CDS             complement(2349..2867)
                     /locus_tag="AC16CY_13765"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3354910.1"
                     /translation="MTDDTKACPVCGETIKAAAIKCRFCNTDLAAFMAAREAEVERDF
                     FVGHPAVIYNAGQWLLVVVTIGIAYIVYWFKSLSTTYQLTSQRVRIERGILSKAKDNI
                     ELFRIDDFEIHKPLGMRLVGHCRLHLLSSDNSTPVAVIYGVPGLEQLADTLRERSLKE
                     RARRKVTTFVDA"
BASE COUNT          523 a          992 c          951 g          493 t
ORIGIN      
        1 cgcgaccttc aggcgtacct gaagctggcc agcatgagcg agacggacaa agaaacgcgc
       61 gcggagcacc agcagatctg ggagcacgtc aagacgctcc ggcggcgcgt cgcggcgctc
      121 aactgaggct gcgcctctca gtccttggtc cttcggtctt ggtcctttgt ccgtcccaag
      181 gtccctggtc cgtcctacgt ccttggtccc aggtccgccg acccgagttc atcacaacgg
      241 ggcagcgatt tgttcaccag aggacgaagg accaaggacc agggacccag gacggaccag
      301 ggacccagga cggacaaaga acaaagtgac taaggaccaa ggaccaactc cacgcgcgtc
      361 accgtgctcc gacgcgcgta ctttggctcg caacgcactt ccagcggccg tcgcggacga
      421 cccagacgtc catgtactga tactgcacct cggtcggctt tccctgcacc ggccccacga
      481 ggtactgcgt gaaccagacg acggcagtgt cgcgatagac cttgatgatg aagtcgtcga
      541 gccggcgtga atcgatctgc tgattctgct ctcgggcgag cgccaactcc tttgccttgt
      601 cgccccggct gccgtcgccg taggtggcga tgaactcgtc ggccaggatg gtctcgatga
      661 acgtggcgtc cccgctgtgg aacgccgcgt cccactgccg ctccaggtcg atcaggattt
      721 cctgatccga cttcacgctc tgcgccaggg cggcgtcgaa ggccgcgagg gaaaggcaca
      781 gcgccagcaa cagcgttctc accgacatga tgcggcctcc ttcagcggtg ccgaggtctg
      841 gtccgacgat tatcatccag cctgcacgat cagccagacg ccgagcaatc cgacgatggc
      901 ggcgatgacg cgccggaact ggcgcggccc gagaccaagc aggatccgct cgccggtaag
      961 cgtcccgacc agcacgcccg ccgtcgcgac ggcaattggc acgcccagcg tgtagagatc
     1021 gccgccggcg cagcacaggt agatgggcgt tctgaccaga tcgaccatca tcgccgtcgc
     1081 tgttgcggtg gccacgtacg gaccgggaga gagggagaac gacagcatcg ccgccgctcg
     1141 caggccaccc tgattgcccg cgaggccgcc gaagacaccc gacagcagcc cgagcacgcc
     1201 ggcgacgggt ccggtgacgc gtaccttcga cggcagatcg ccgagcgtcg cagcggacgt
     1261 ggccagcagc agcaccgcga gcgtcagcgt caacgaggcg ttcgacagcc gtgtgtagag
     1321 cagggcgccg gcgaggctcc cggcggcgct gaggagcccg aagcggcgaa ggacggtcca
     1381 atccacggcc tttcgcagcc gccagcatct cagtgccgtg gcggccgcat gcggaatcgc
     1441 gacggcggcg acggccgtgc ccgtgtcgaa gcgcgccgcg agcagcggcg tcaggaggct
     1501 gccgatcccg aacccggcga cggcggcagt gccgccgctc aggacggcga gagcgaacac
     1561 gccgagcagg aacatgtcac ttcggccgcg tgtccttctc gttctcgagg cagatgaact
     1621 ccatgagctc cgtgttcggc agcagcgtca cttcgaccgt cgcgccccac ggcttcgtgt
     1681 acgccttggg atcgtcgatc gtgatgccga tttccatgtg cccgaagtct ttccgccgga
     1741 agcgcgtcgt gacgtgaagc gcttcggtgg acggcttgcc caactggtcc agccacaccc
     1801 ggccgttgaa gccctggctg tccaccacga gcgtgtcgcc ctcccatttc gcggtcgagt
     1861 acccgtgcca cgtcggctgg acgtccgggt gtggaacgaa ctgacggccg tccatgaacg
     1921 cctgccacca gagattgaac gcctcgtgga tgaagtacac gacgcgcggt gtctgcacga
     1981 tgcgccaggg cgccggcgcg agcagcacct tcggcactcc ctgcggcagg cagttcgcgt
     2041 cgggctccga cttccagtgg atcccggcgg cgcgttcgtc gtacaccgcc ttggcccatg
     2101 gctggaacgg cacctcctcc ttgaggtcgc gggcgagatt tctcaagtat ccggtcggcg
     2161 ggttccagat gcctgaaagg tccggcttgc cgtcgggcag acgcggcgcc ggggccgaca
     2221 ggttcggctt gccgtcgggc gtcaggggaa cctggggagg gggaatcttc gtccactgcg
     2281 cgaatgccgg tgcggcgacg agcgccacga ccgcgagctg aaggaacgtc ctcatgtcga
     2341 cccccgttct acgcatcaac aaacgtcgtg acctttcgcc tcgctcgctc cttgagcgaa
     2401 cgttcccgca gcgtgtccgc aagctgctcc aggccgggga cgccgtagat cacagccacg
     2461 ggagtgctgt tgtcggacga aagcaggtga agccggcagt ggccgacgag cctcatcccg
     2521 agcggcttgt ggatctcgaa gtcgtctatc cggaacagct cgatgttgtc cttggcctta
     2581 gagaggatgc cccgctcgat cctgacccgc tgactcgtca gctgatacgt cgtggaaagg
     2641 ctcttgaacc agtagacgat gtaggcgatg ccgatcgtga caacgaccag gagccattgc
     2701 cccgcgttgt agatgacggc cgggtggccg acgaagaagt cccgctcgac ctcggcctcg
     2761 cgcgccgcca tgaacgccgc cagatcggtg ttgcagaagc ggcacttgat cgcggccgcc
     2821 ttgatcgttt ctccgcacac ggggcacgct ttggtgtcgt cagtcatgct cgtcccttcg
     2881 ggcgctcgaa ccgcatccct cttcgatcag atagtctcct tcaggcgagc ggccacgtat
     2941 ccggctttcc tcatccctt
//
LOCUS       DBNGSH010000595         2529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1927214, whole genome shotgun sequence.
ACCESSION   DBNGSH010000595 DBNGSH010000000
VERSION     DBNGSH010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1927214"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC16CY_13770"
     CDS             <1..237
                     /locus_tag="AC16CY_13770"
                     /inference="COORDINATES: protein motif:HMM:NF019982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3354911.1"
                     /translation="PPGWGDSVVKHLSRGLHITAPGTGHGVVTTACGNRLVSDFIDRG
                     SIDNLDTECIAAIRRPPFFVTPAGPALTQRAAGK"
     gene            234..968
                     /locus_tag="AC16CY_13775"
     CDS             234..968
                     /locus_tag="AC16CY_13775"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3354912.1"
                     /translation="MIRIEDVRKHFGSVAAVDGVSFTAENGAITGLLGPNGAGKTTTL
                     RMLYAVMAPDSGQIHVDDIDAVASPQTAQARLGVLPDGFGLYPRLTAREHLEYFGALH
                     DLGGAHLARRIDELLDLLDMRGIADRRTAGFSHGERTKVALARALVHDPQNVLLDEPT
                     NGLDVMSTRAVRAIVGRLRDEGRCVLFSSHVMQEVSALCDSIVVMSRGKVAAVGSPDD
                     LRRQTGHDNLEDAFVKLADRRRRPSA"
     gene            965..2152
                     /locus_tag="AC16CY_13780"
     CDS             965..2152
                     /locus_tag="AC16CY_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354913.1"
                     /translation="MTGLRAVRQAAIVCRKELRDSLRDRRALWSILFSVIIGPVLIGF
                     MMNRIIDRQREAENVVIPIVGMNRAPALVDWLRQQAGVTVEEGPADAERAVKERREAV
                     VVVIPENFAERFADSKPATVRVVADGSRNDTRAQGERVRRLLAVYAAEIGSLRLIVRG
                     VSPAAVRPLAIEDVEVSTAQQRAAQILTFIPMFIMVAGFVGGMQIATDSTAGERERGS
                     LEPLLVNPAPRASFVTGKWLAASVAAIGSAILTTALCAQLTKLLPLEDMGVSLRLGPD
                     YFGYVLLAAIPLCLFTAALQATVATMSRSFKEAQSYMGVLMLGPMLPGLVASLYPMGS
                     TLWLNGIPMLGSFLLLSDTLSGRPPGAAEFLTSAAVSFALTVGLLRVTTRLFRSEHII
                     FGR"
BASE COUNT          404 a          850 c          842 g          433 t
ORIGIN      
        1 ccgccagggt ggggcgactc cgtcgtcaag catctcagcc gcgggctgca catcaccgct
       61 ccgggcacgg ggcacggcgt cgtgacgacg gcgtgcggca accgcctggt gagcgacttc
      121 atcgaccgcg gttcgatcga caaccttgac accgagtgca tcgccgccat ccggcgacca
      181 ccgttcttcg tcacgccggc cgggccggcg ctgacgcagc gggcggccgg caaatgatca
      241 ggatcgagga cgtccgcaag catttcggca gcgtcgcggc cgtggacggc gtgagcttca
      301 cggccgaaaa cggggcgatt accgggcttc tgggaccgaa cggcgcaggc aagacgacga
      361 cgctccgcat gctctatgcg gtgatggcgc ccgacagcgg acagattcac gtggacgata
      421 tcgacgccgt cgcttcgccg caaaccgcac aggcgcgcct cggcgtcctg ccggacgggt
      481 tcgggctgta cccgcgcctg accgcgcgcg agcacctgga gtacttcggc gcgcttcacg
      541 acttgggcgg cgcgcacctc gccaggcgga tcgacgagct gctcgatctg ctcgacatgc
      601 ggggaatcgc cgaccggcgg accgccggct tctcgcacgg agagcgcacg aaagtggccc
      661 tcgcgagggc gctcgtccac gatccgcaga acgtgctgct cgacgagccg accaacgggc
      721 tggacgtgat gagcactcgc gccgtgcgcg ccatcgtcgg ccggctgcgc gacgagggcc
      781 ggtgcgtgct gttctcgagc cacgtgatgc aggaggtgtc ggcgctgtgc gactcgatcg
      841 tcgtgatgtc ccgcggaaag gttgcggccg tcggctcccc cgatgacctt cgccggcaga
      901 ccgggcacga caacctcgag gacgccttcg tgaagctggc cgatcgcagg cggaggccgt
      961 ccgcgtgacc gggctccgcg cggtgcgtca ggcggccatc gtctgcagga aggagctgag
     1021 ggactccctg agggatcggc gcgccctctg gtcgatcctg ttcagcgtga tcatcggtcc
     1081 cgtgttgatc ggtttcatga tgaaccgcat catcgaccgg cagcgcgagg ccgaaaacgt
     1141 cgtcattcca atcgtcggca tgaaccgggc accggccttg gttgactggc tgcggcagca
     1201 ggcgggcgtg acggtggagg aagggccggc cgatgccgaa cgggcggtca aggaacgccg
     1261 cgaggccgtc gtcgtggtca ttcccgagaa cttcgcggaa cggttcgccg actccaagcc
     1321 cgcaaccgtc cgcgtcgtcg ccgacggctc gcgcaacgac acccgcgcgc aaggcgaacg
     1381 ggtgcgccgc ctgctggcag tctacgcagc cgagatcggc agcctgcgcc tgatcgtccg
     1441 cggcgtgagc cctgccgccg tcaggccgct cgcgatcgaa gacgtggagg tgtcaaccgc
     1501 gcagcagcgc gccgcgcaga ttctgacctt catcccgatg ttcatcatgg tggcgggctt
     1561 cgtcggcggc atgcagatcg ctaccgattc gacggcgggc gaacgcgagc gcggatcgct
     1621 cgagccgttg ctcgtgaacc cggcgccgcg cgcgtcgttc gtcaccggaa aatggcttgc
     1681 cgcttcggtg gccgcgatcg gcagcgccat cctgacgacg gcattgtgcg cgcagttgac
     1741 aaagctcctg ccgctcgagg atatgggcgt gagcctgcgc ctcggccccg attacttcgg
     1801 gtacgttctg ctggcggcca tcccgttgtg cctgttcacg gccgccctcc aggcgacggt
     1861 ggcgacaatg tcgcggtcct tcaaggaggc gcaaagctac atgggcgtcc tcatgctcgg
     1921 cccgatgctc ccgggcctgg tcgcctcgct ctatccaatg ggcagcacgc tctggctgaa
     1981 cggcataccg atgctcggct ccttcctgct tctctcggac accctgagcg gccgtccgcc
     2041 tggcgccgcc gaattcctga cgtcggcggc cgtctcgttc gccctgaccg tgggccttct
     2101 tcgagtgaca acgaggctct tccgaagcga acacatcatc tttgggcgct gattcctgaa
     2161 gggtccggct aaagccggac actacttatg tctctcctta tgtctctccg taatggctga
     2221 gcttgatcgg ttgacgtacg tagtgtccgg cttcagccgg acccaaagaa gtagtgtccg
     2281 gctttagccg gaccaggcca cacaagatgg ccaatgtgca cgttgtgtgc agtcgcaagc
     2341 gattcctgcc gataggctgt ccatcatggc attcgaggct gcgcgccagg acgaaggcag
     2401 cgcgtattct tcccgaccga tcgcggggcc ggtccgcctg acgcccgggc tcttcgacgc
     2461 atcgccctcg acgcccgcct ctctgaccta cgagcactac tacgggctga aagaaaaggc
     2521 gttcagcct
//
LOCUS       DBNGSH010000596         8347 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1757983, whole genome shotgun sequence.
ACCESSION   DBNGSH010000596 DBNGSH010000000
VERSION     DBNGSH010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8347)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1757983"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="AC16CY_13785"
     CDS             complement(<1..667)
                     /locus_tag="AC16CY_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3354914.1"
                     /translation="MRTLFVALIVAAALAMPAGVASQQPPPQGPTFKGGVELITVDVG
                     VVDRNGRPVEDLTPADFTVKINGETRRVVTADLVKVDLEQARKRAADPAEAYYSSNIA
                     PVNGRQIIIAVDQLYISPSGVKPIMNAAVQFLNRLTPLDSVAFVAFPEPGPRANFTTD
                     RFRVRQAMQGIVGQNTMKATLINMGVSEALDINNRRDQNVLRDVSGRVCSNAVDATER
                     DRCV"
     gene            complement(716..3187)
                     /locus_tag="AC16CY_13790"
     CDS             complement(716..3187)
                     /locus_tag="AC16CY_13790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354915.1"
                     /translation="MSVLTDLRIGLRLLWKDKAFTVTAVLTLALCIGANTALFSVVRG
                     VLLKPLAIPEPDRVVNAGNTYPGAGVSRAIGASVPDYFDRLREVTAFEEQALLDSVSY
                     SVVQSGVPARVEAMSVTPSFFRLAGVKPLLGRTFSEQEGEVGNTQVVVLSDGFWRSQL
                     GGDPGIVGRELRLDGVPHTVVGVMPARFRFPSDDEALWTPMSFTPEARADDKRHSNNA
                     LYLARLKPGATLEQAQAQIDTLNRANLDRFPALKEIIINTRFRTVVQPVTDLIVEDVR
                     PVLYLLWAGALFVLLIGCVNVANLVLVRSRVRVKELATRLALGAGTWRLAQQLVIENL
                     ILTFVSAAAGLAVGAAALQAMGAINLEDLPRSAEIALDGSVALYTVGVAAAIGLVLGT
                     IPLLGAMPSNLVGVLRVEGRTGTSAAGARTLRRGLVIAQVACAFVLLIGAGLLFASFR
                     QVLKVDAGFGSEGVLTGAVSLPDSRYKDDDARRRFTSETLRRLREQPGVSKAGATDTI
                     PFGNNQSASAILAEGYQAKPGESIVAPNEVRVSDGYFEVMGARLVGGRFFTERDGAGA
                     PRVAVIDERLARRFWPQQDAVGRRLYRPNDDGPDLTAITAKTEFITVVGVIREMKLRR
                     LTDGDQLVGAYYLPLAQEPQDDVTFALKAGGDPAALAGTLRRTVTALDSELPVFEMQP
                     MTYWVDKSVATHRSPALLSVGFGVVALLLSAIGIYGVLAYLVAQRTKEIGIRIALGSS
                     APAVFWLVLREGVLLVAAGLVIGGAGTILLGRALESLLFGVRTSDPLVLVTVSLLLAA
                     VAFTACAVPARRATAIDPLTALTDS"
     gene            complement(3229..3729)
                     /locus_tag="AC16CY_13795"
     CDS             complement(3229..3729)
                     /locus_tag="AC16CY_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354916.1"
                     /translation="MRRTRLLCLIGVSLWSAVVAGSQAATSGALSPALRAHVKGERFQ
                     IVESIRGLPLGVRDALQTLWGTTTLDIAEPGTEFQATDVIVNPTVNPKLPIRRLVAAG
                     CSTDHCLVYYERGGRARTWHVTLYHWTPAATRLEGGGSAPGGLKTVDDLRNLVLSGAM
                     GASKVW"
     gene            3875..5062
                     /locus_tag="AC16CY_13800"
     CDS             3875..5062
                     /locus_tag="AC16CY_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354917.1"
                     /translation="MDSTVLTPLLAMTAWFLGLSILVQVLQELWKFVTSSKARSFEKA
                     LADFLGPFIVGRLQQDPLLAVRGPLQFRRVSTAGHVLPLDAADLSASLEKSAPEWQRL
                     VRRALAFEASAQRSEPAPPTPAFLDTVRALERQVTDARALDDRRLSAEALALGDAGNV
                     YAFLEKWNVTSETARPKKLDAATVQLAFVREFFPQEETISRHYDQFLENFGFQYRRRN
                     LRQTFTFAMLVAVMLNLPFEQIYLRATMVSPEEAIALAENAQKLYEQAATSTDQASRQ
                     QLETFGKQALDIARSAADFACTPRDADLESPTAEGATTPAPVGCDVALSVDYFLDPAV
                     VWRRMTDRQTVAPRFLFGCMLTAILITFGAPFWNDLSSTLLRVARPTGARGPDRTVKL
                     EVE"
     gene            5067..6425
                     /locus_tag="AC16CY_13805"
     CDS             5067..6425
                     /locus_tag="AC16CY_13805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3354918.1"
                     /translation="MAAEFIWPSLHGVRGPGFWLNPVHWIAARKQFRRDHTVEWRQRY
                     ENDAPTAPDRVGDFRDLLSSPDLDGGFSFLILGDTGEGDRSQYGLLPLIRGLDPDFII
                     INGDLAYPAGSEKDFVEGFFQPYQNLRMPIWAVPGNHEYYSPHNGREFFDLFCTTKHT
                     RLWQDHGLRFVRQPGTYWELSSPDCPLVVLALDSGMTASLDPAGKLFGLLKSDGDSAQ
                     LAWLERRLAVAQASGKRVLVIFHIPSLVNLKKAGQPKLDGLHGILGRFSQTITGVVTA
                     HEHSFQHYAPDQFARFLSGGTMPGPHYFVSGSGGAFLSPIEFEAAHGLFAASHLFPNR
                     KQWKDYARLSKKAASALGLARTALARAISSLSGALADRDVARYQSLLRVQVTPAAGGG
                     FTATMTPYFQNSLEELYFDQPNATVVVRDGTPRPSRVAMERLTRRHPDEPGTEISVQL
                     AP"
     gene            complement(6410..7690)
                     /locus_tag="AC16CY_13810"
     CDS             complement(6410..7690)
                     /locus_tag="AC16CY_13810"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354919.1"
                     /translation="MAFLARALAVASVVWLAPAAFAHHGFANFDLNTELEISGTVTSL
                     AFVNPHSWLHLNVTGKDGQTSAWRCELRGATVLKRSGWSPEMFTPGTRVTITGSPDRF
                     EPNTCYLGTVVFADGTRIDRYGQIKRPAPAPTPAERPARLANGRPNFSGDWAAEQRVL
                     TDPRGMSGAFLPIGVAQTLKPGDVPEGTQAFPGTRGSAVSLAEDPIEAFWNRPSAIPL
                     TEAGAKAIEGFDGSSADNPRLRCETTNILFDWTFESDVNRITQGDDTLTMLYGSMALM
                     RTVYLNMAEHPKDVRPSRAGHSIGRWEGDTLVVDTIGFEPGILSADGRIPHSDRLHVV
                     EQFSLDAAKPALMRSFVAEDPLYFQGQYKGADIVYVADVPYQGTRCDDRSYRSPGQSG
                     GARWILWTSMAVVVGAVAIMWTVTARRRRVQGAS"
     gene            complement(7691..>8347)
                     /locus_tag="AC16CY_13815"
     CDS             complement(7691..>8347)
                     /locus_tag="AC16CY_13815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3354920.1"
                     /translation="ALRAGADGLMHGIIDEPVDQEFLDLMKRNKAVYVATSSLYEDVA
                     DVASWARRQAAYDEAGRLSAIVNGFIAGPGAQQFEATFNNSALTKRLLPTQRANLKKV
                     FDAGVPVVMGTDAGFLGILMGVSSQAELALMVEAGLTPDAALAAATINAARMLGREAT
                     FGTIETGKQADMIVLDADPLADIGNVRRIHRVIKGGVVHDPGQLLAGMRTLPPAGVDK
                     "
BASE COUNT         1414 a         2864 c         2746 g         1323 t
ORIGIN      
        1 ggacgcatcg atcgcgctcc gtcgcgtcca ccgcgttgct gcagacgcgg ccgctcacat
       61 cccggagcac gttctgatcg cgccggttgt tgatgtccag ggcctcggag acgcccatgt
      121 tgatgagcgt cgccttcatg gtgttctgcc cgacgatgcc ctgcatcgcc tggcgcacgc
      181 ggaacctgtc ggtcgtgaag ttcgcgcgcg ggccaggctc gggaaacgcg acgaaggcca
      241 ccgagtcgag cggcgtgagc cggttgagaa attgaaccgc cgcgttcatg atcggcttga
      301 cgcccgatgg ggaaatgtag agctgatcga cggcaataat gatctgacgg ccgttgacgg
      361 gagcgatgtt gctcgagtag tacgcctcgg cgggatcggc ggcccgcttc ctggcttgtt
      421 cgagatcgac cttgaccaga tccgccgtta ccacgcggcg ggtctcgccg ttgatcttca
      481 ccgtgaagtc ggccggggtc agatcctcga ccggccgtcc gttccggtcc acgaccccga
      541 catcgaccgt gatcagctcg acgcccccct tgaacgtcgg cccctgggga ggcggctgct
      601 gcgaggccac gccggcaggc atggcaagag cagcggcgac gatcaacgca acgaagagcg
      661 ttcgcatgcg cggatcctac accctctcga gatacccggg gtcggaaaga gagagctacg
      721 agtcggtcag cgcggtgagt ggatcaatcg ccgtggcgcg acgggcaggc acggcgcagg
      781 ccgtgaacgc gaccgcggca agcaggaggc tcactgtcac gaggacgagc ggatcgctcg
      841 tcctcacacc aaataacagg ctctcgagcg cccgtcccag caggatcgtt cccgccccgc
      901 cgatgacgag gccggcggcg acgagaagca ccccctcgcg caggacgagc cagaagaccg
      961 ccggcgcgct gctgcccagc gcgatccgga tgccaatctc cttcgtccgc tgcgcgacca
     1021 gatacgcgag cacgccgtag atgccgatgg ccgacagcag cagggcgacg acgccgaagc
     1081 cgaccgacag cagcgccggt gaacggtgcg tggcaaccga cttgtccacc cagtacgtca
     1141 tcggctgcat ttcgaacacc ggcagctctg aatcgagcgc ggtgacggtg cgccgcagtg
     1201 tgccggcgag tgcggcagga tcgccgccgg ccttcaacgc gaacgtcacg tcgtcctgcg
     1261 gctcctgcgc gagcggcagg tagtaggcgc cgacaagctg gtcgccgtcg gtcagccggc
     1321 ggagcttcat ctcgcgaatc acaccgacga cggtgatgaa ctccgtcttg gcggtgatcg
     1381 cggtgaggtc ggggccgtcg tcattgggtc gatacaaccg gcggccgaca gcatcctgct
     1441 gaggccagaa cctgcgcgcg agccgttcgt cgatcacggc gacgcgaggg gcacccgctc
     1501 cgtcgcgctc ggtgaagaac cggccgccga ccagccgggc gcccatgacc tcgaagtacc
     1561 cgtcggagac gcgcacttcg ttaggggcga cgatggactc gccgggcttt gcctggtaac
     1621 cctccgccag gatcgccgag gcgctctgat tgttgccgaa cgggatcgtg tcggtcgccc
     1681 ccgctttcga aacgcccggc tgctcgcgca gccggcgcag cgtctcgctc gtgaagcgtc
     1741 ggcgggcatc gtcgtccttg tagcgagagt cgggcagcga gacggcccca gtcaggacgc
     1801 cttcgctgcc gaagccggcg tccaccttca gcacctggcg gaagctggca aagagcaacc
     1861 ccgcgccgat cagcagcacg aacgcacagg ccacttgggc gatgacgagg ccgcggcgga
     1921 gcgtccgcgc accagccgcg ctcgtcccgg tgcgcccctc cacgcgcagg acgccgacga
     1981 ggttcgacgg catcgccccg agcagcggaa tggtcccgag cacgaggccg atcgcggcgg
     2041 cgacgcccac ggtgtacagc gcgacgctgc cgtcgagcgc gatctcggcc gaacgcggca
     2101 gatcctcgag gttgatcgcc cccattgcct gcagcgcggc agcaccgacg gcaaggcccg
     2161 ccgcggccga gacgaacgtc agaatgaggt tttcgatcac gagctgctgc gccagccgcc
     2221 acgttccggc cccgagcgcc aggcgcgtcg ccagctcctt cacgcgcacg cgcgatctga
     2281 ccagcaccag gttggccacg ttgacgcagc cgatgagcag cacgaacagc gcgccggccc
     2341 acaacaagta cagcaccggc cgcacgtcct cgacgatgag gtcggtgacc ggctgcacga
     2401 ccgtacggaa tctggtgttg atgatgatct ccttgagcgc ggggaagcga tcgaggttcg
     2461 ccctgttgag cgtgtcgatc tgcgcctgcg cctgctcgag cgtggcgccc ggtttcaaac
     2521 gggcgaggta cagtgcgttg ttgctgtgac gcttgtcgtc tgccctggct tcgggggtaa
     2581 acgacatcgg cgtccacaac gcttcgtcgt ctgatggaaa tcgaaaccgt gcgggcatca
     2641 cgccaacgac ggtgtgaggg acgccgtcga gcctcagctc ccgacccacg atgcccggat
     2701 cgccgccgag ttggctgcgc cagaatccgt cgctcagcac gacgacctgc gtgttgccga
     2761 cctcaccctc ctgctcggag aacgtacggc cgagcagagg cttgacacca gccagccgga
     2821 agaacgacgg cgtcacgctc atcgcctcga cgcgcgcggg aacgccgctc tgaacgacgc
     2881 tgtagctcac ggaatcgagc agcgcctgct cctcgaaggc ggtgacctcg cgcagccggt
     2941 cgaaatagtc cggaacagag gccccaatcg ccctcgagac gcccgctccc gggtacgtgt
     3001 tgcccgcatt gacgaccctg tcgggctccg ggatcgccag cggtttgagc aggacgccac
     3061 gtaccaccga gaagagcgcc gtgttggcgc cgatgcacag ggccagcgtc aggacggcgg
     3121 tgaccgtgaa ggccttgtcc ttccagagca gccggaggcc tatgcggagg tcggtgagaa
     3181 cgctcatgtg cttcttggac gcaggccgga cggccggagt tcagggaact accagacctt
     3241 gctcgcgccc atggcccctg acaggacgag gttccggagg tcgtcaacgg tcttcagccc
     3301 gcctggcgcg ctgccgccac cctcaaggcg agtcgccgcg ggcgtccagt gatacagcgt
     3361 cacgtgccag gtgcgggcgc ggccgccccg ttcgtaatac acgagacagt gatcggtcga
     3421 gcagcccgcc gccaccagcc gacggattgg cagcttcgga ttgacagtcg gattgacgat
     3481 cacgtctgtc gcctgaaact cggttccggg ctcggcgatg tcgagtgtag tggttcccca
     3541 caacgtctgc agcgcgtcgc gcacgccgag cggcagtccc cggatggact cgacgatctg
     3601 gaaccgctcg cccttcacgt gagcccgcag cgccggcgac agtgcaccgg aagtggccgc
     3661 ctggctgccg gcaaccacgg cggaccagag gctcacgcca atcaggcaga ggaggcgcgt
     3721 gcgacgcatc gaggaaccag cttacaccta taatcctggg cgcttgaatc caactcccaa
     3781 ctcccaactc ccgactccca accgttacgt tgccgttgga ggttgggaat tgggcgttgg
     3841 aagttgacac acgcgcgctc gaggaggccc ccacatggac tcgacggtgc tcacgccgct
     3901 gctggcgatg acggcatggt ttctcggcct ctccatcctc gtgcaggtcc tgcaggagct
     3961 ctggaagttc gtcaccagca gcaaggcgcg ctcgttcgag aaggcgctcg ccgactttct
     4021 cggaccgttc atcgtggggc ggctgcaaca ggatccgctg ctcgccgttc gagggccgct
     4081 gcagttccgg cgcgtcagta ccgcgggcca cgtgctcccg ctcgacgcgg ccgacctgtc
     4141 ggcctcgctc gagaagagcg cgcccgaatg gcagcggctt gtcaggcggg cgctcgcctt
     4201 cgaggcgtcg gcccagaggt cggagccggc gccgcccacg cccgcgtttc tcgacacggt
     4261 tcgggcgctc gaacgtcagg tcacggacgc gcgtgcgctc gacgatcggc gcctcagcgc
     4321 cgaagccctg gcactcggcg acgcgggaaa cgtctacgcg ttcctggaga aatggaacgt
     4381 cacgagcgag accgcgcggc ccaagaagct cgatgccgcg acggtgcagc tggcgttcgt
     4441 gcgggagttc tttccgcagg aggaaacgat cagccgccac tacgatcagt tcctcgagaa
     4501 tttcggattc cagtaccggc ggcggaacct gcggcagacc ttcacgttcg ccatgctggt
     4561 ggccgtgatg ctcaacctcc ccttcgagca gatctatctg agggccacga tggtctcgcc
     4621 cgaagaggcg atcgccctcg ccgagaatgc gcagaagctc tacgagcagg cggccacctc
     4681 gacggatcag gcatcgcgcc agcagctgga gacgttcggg aaacaggcgc tcgacatcgc
     4741 gagaagcgcg gccgactttg cctgcacgcc ccgcgacgcg gacctcgagt cgccaaccgc
     4801 cgaaggcgcg accaccccgg cccccgtggg ctgcgatgtc gcgttgagcg tggactactt
     4861 cctggatcca gcggtcgtgt ggaggcggat gaccgaccgc cagacggtcg ccccacgctt
     4921 cctcttcggt tgcatgctga cggcgatcct gatcaccttc ggcgcgccgt tctggaacga
     4981 cctgagttcg acactgctcc gggtcgcgcg gcccacgggc gctcgaggcc ccgaccggac
     5041 agtcaagctg gaggtggagt agccgcgtgg cggccgaatt catctggccg agcctgcacg
     5101 gcgttcgcgg gcccgggttc tggctcaacc cggtgcactg gatcgccgcc cgcaagcagt
     5161 tccggcgcga ccacactgtc gagtggaggc agcgctacga gaacgacgcg cctacggcgc
     5221 ccgatcgcgt gggcgacttc cgcgatctcc tctcgtcgcc ggatctcgac ggcggattct
     5281 cgttcctcat cctcggcgat accggagagg gcgaccggtc gcagtacggt cttctgccgc
     5341 tcatccgcgg cctcgacccc gacttcatca tcatcaacgg cgacctcgcc tacccggccg
     5401 gcagcgagaa ggacttcgtc gaaggcttct tccagccgta ccagaacctg cggatgccca
     5461 tctgggcggt ccccggcaac cacgagtact actcgccgca caacgggcgt gagttcttcg
     5521 atctcttctg cacgacgaaa cacacgaggc tctggcagga ccacggcctg cgcttcgtgc
     5581 gccaacccgg cacctactgg gagctctcct cgcccgactg cccgctggtc gttctggccc
     5641 tcgactccgg catgacggcg tcgctcgacc cggccgggaa actgttcggc ctgctgaagt
     5701 ccgacggcga cagcgcccag ctggcgtggc tcgagcggcg gctcgccgtg gcgcaggcca
     5761 gcggaaagcg agtgctggtc atcttccaca ttccgtcgct cgtcaatctc aagaaagcgg
     5821 gccagccgaa gctcgatggg ctgcacggca tcctcggacg gttctcgcag acgatcaccg
     5881 gcgtcgtcac ggcccacgag cacagctttc agcactacgc gccggaccag ttcgcgcgtt
     5941 ttctgtcggg cggcacgatg ccggggccgc actacttcgt ctcgggatcg ggcggcgcgt
     6001 ttctctcgcc gatcgagttc gaggcggcgc acggcctgtt cgccgcgagc catctgttcc
     6061 cgaacaggaa gcagtggaag gactacgcgc gcctctcgaa gaaagccgcc tcggcgctcg
     6121 gcctggcacg aacggccctg gcacgcgcca tcagcagcct ctcgggagcg ctggccgacc
     6181 gggacgtggc gcgctatcag agcctgctgc gagtgcaggt cacgccggcc gcgggcggag
     6241 gcttcacggc cacgatgacg ccgtatttcc agaacagcct cgaggagctc tacttcgacc
     6301 agccgaatgc gaccgtggtc gtgagagacg gcacgccgag gccgtcacgc gtggcgatgg
     6361 agcggttgac ccggcgccat ccggacgaac cgggcaccga gatcagcgtt cagctggcgc
     6421 cctgaacacg ccggcgtcgc gccgtaaccg tccacatgat cgcgactgcg ccgacgacga
     6481 ccgccatgct ggtccaaagg atccaccgag cgccgccgga ctggcctggc gacctgtagg
     6541 atcgatcgtc gcacctggtg ccctggtacg ggacgtccgc gacgtagacg atatcggctc
     6601 ccttgtactg tccctggaag tagagcggat cttccgcaac gaagctgcgc atcagagccg
     6661 gcttcgcggc atcgagcgag aactgctcga cgacgtggag tcgatcgctg tgcgggatac
     6721 ggccgtcggc cgacaggatg ccgggctcga atccgatcgt gtcaacgacc agcgtgtcgc
     6781 cctcccatcg gccgatcgaa tggcccgccc ggctcgggcg aacgtccttg gggtgttcgg
     6841 ccatgttcag gtacacggtc cgcatcagcg ccatcgaccc gtaaagcatc gtgagggtgt
     6901 cgtcaccctg ggtgatgcga ttgacgtccg actcgaacgt ccagtcgaag agaatgttcg
     6961 tcgtctcgca gcgcaggcgc ggattgtccg ccgacgaacc gtcgaaccct tcgatcgcct
     7021 tcgcgcccgc ctcggtcaac gggatcgcgc tcggccggtt ccagaacgcc tcaatcggat
     7081 cctcggcaag cgacacggcg gacccccgcg tgcccggaaa cgcctgcgtg ccttcgggca
     7141 cgtcgcccgg tttcagggtt tgcgccacgc caatcggaag gaaggcgccg ctcataccgc
     7201 gcggatcggt cagaactcgc tgctcggcgg cccagtcgcc cgagaagttc ggcctgccgt
     7261 tggcgagcct cgccggcctc tccgcgggcg tgggcgccgg cgccggccgc ttgatctgac
     7321 cgtagcgatc gattcgcgtg ccatcggcga agaccaccgt cccgagatag caggtgttcg
     7381 gctcgaagcg atccggcgat ccggtgatcg tcacgcgcgt gccgggtgtg aacatctcgg
     7441 gcgaccagcc cgatcgcttg aggacagtcg ctccgcgcag ctcgcatcgc cacgcgctgg
     7501 tctgaccgtc cttccccgtc acgttcagat ggagccagga atgcgggttc acgaacgcga
     7561 ggctggtcac cgtgccgctg atctcgagct cggtgttgag atcgaagttg gcgaagccgt
     7621 gatgcgcgaa agcggccggc gcgagccaga cgaccgacgc gacagcgagc gcgcgcgcga
     7681 ggaaagccac ttacttgtcg acccccgcgg gcggcagcgt ccgcatgccg gcaagcaact
     7741 ggccgggatc gtgcaccact ccccccttga tcacccgatg gatacgccgg acattcccga
     7801 tgtccgcgag cgggtcggca tcgaggacga tcatgtccgc ctgcttgccg gtctcgatgg
     7861 tgccgaacgt ggcttcccgc cccagcatgc gcgcggcgtt gatcgtggcg gcggcaagcg
     7921 cggcgtccgg cgtcaggcca gcttcgacca tcaacgcgag ctcggcctgg ctggacactc
     7981 ccatcaggat ccccaggaac cccgcatcgg tgcccatcac gacgggtacg ccggcatcga
     8041 agaccttctt gagattggcc cgttgcgtcg gcagcaggcg ctttgtcaac gcgctgttgt
     8101 tgaacgtcgc ctcgaactgc tgcgcgcccg gtccggcgat gaagccgttg acgatcgcgg
     8161 acaggcggcc agcctcgtcg taggcggcct ggcgccgcgc ccacgaggcg acgtccgcga
     8221 cgtcctcgta gagtgacgac gtggccacgt acaccgcctt gttccgtttc atgagatcga
     8281 ggaactcctg atcgaccggc tcgtcgatga tgccgtgcat caacccgtcc gcgccggcgc
     8341 gcagcgc
//
LOCUS       DBNGSH010000597         6920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3795280, whole genome shotgun sequence.
ACCESSION   DBNGSH010000597 DBNGSH010000000
VERSION     DBNGSH010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3795280"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="AC16CY_13820"
     CDS             <1..1687
                     /locus_tag="AC16CY_13820"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3354921.1"
                     /translation="ILLEHGADPNRTGAGGTTALMWAVPDLGKVRLLLARGANVNAAS
                     ETGRTALLVAASYPRTVEVLQLLLERGANLRAQDRTGATALGFAARSADVDVVRFLVA
                     RGLDAQSLSPGARRAAFARNDLRTSDYLIAAAPMPAQDDLLVAAIWQPARLVSRWIEL
                     GADVNASAPAQYARTPLMNAVTSEAEGADTVRLLLEKGANPNAQMTEGERPLDWAIYK
                     GDKAKIQALEQYGATRGQGPRQAVIAPPATGGGVDARTSLARSLTRLMEVAPKFRDQA
                     TCISCHHNTMPALAAAAARTKGVPVDEPRARKNVEDLRTFFAGNVARMMLGDPAVGGE
                     ALTTGYAQMALAAEGHPLDTVTASMTHWLMARQMPNGSWLGNGLNRPPSEYSLVSHTA
                     IAAGGLKAYPLPGRSGEVAESVRRAGQWLLAAKATSAEERGMRLMGLVWTGAPRSAVE
                     AAIREIRERQEASGGWSQFGRTEPDAYATGLSLYALHVAGVRATDETYRKGVAFLLST
                     QYPDGTWLVKTHSFPVQRYFESGFPYGRHQWISTAGTSWASLAIAHTLPDARK"
     gene            1703..2128
                     /locus_tag="AC16CY_13825"
     CDS             1703..2128
                     /locus_tag="AC16CY_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354922.1"
                     /translation="MAGIRMGKVVLGGLAAGLIINIGETILNIPLLGAEMEQALQARN
                     LPAVGGGAIAYFVGVCFLLGIVIVWTYAAVRPRLGPGPKTAVMVAVLVWLLSFVWAGG
                     AQAAMGIMPLKLTVVGLVWGLGELVIATLVGAKLYTETA"
     gene            complement(2150..3577)
                     /locus_tag="AC16CY_13830"
     CDS             complement(2150..3577)
                     /locus_tag="AC16CY_13830"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3354923.1"
                     /translation="MTTAIRAFAGLVAITLAAGVLVAQQNSVVPQPRAEALPAPGAAS
                     PKPSRSVNRPEGITPRVPAGFTVTSYAELRAPRMMVYAPNGDLFVSSPGANSITVLRD
                     ANNDGVFEAQGVFAQGEPPAPRGGGAAPQGGARPGGAPGGAAPGGAPGRGAAGAGPPP
                     GGGRGAPPVLGADAPACTPPPEFAVRGPGTLAAPFGLAFHDGYLYVGNTASLVRYKYS
                     SGDLQAQGQPEKLLDLPAGGHSTRNIIFNRAGTKMYVAVGSQSNNNAGEDCRRAAILE
                     FNPDGTGYRVFASGIRNPVGLALQPGTDIVWTAINERDNLGDDLVPDYATSVKDGAFY
                     GWPYSYIGSNYDPRYIGSFPDLVKRATVPDVLLPSHAAALGIAFYTGTQFPQRFRNGG
                     FVALHGSWNRSVASGYKVVFFPMVNGKPGPIEDFLTGFLASDGSNGTPIEQWGRPVGV
                     TVARDGALLVSDDSGNRIWKITAAK"
     gene            complement(3590..4381)
                     /locus_tag="AC16CY_13835"
     CDS             complement(3590..4381)
                     /locus_tag="AC16CY_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354924.1"
                     /translation="MIEIRTSIPRCLLAIALVAATAASGEAQWEPYPWKNMPRTADGK
                     VDMNASPRRTPDGKVDLSGFWMPVEAVKHLLNLAADLKPGSVPLRPWAEALYKERIEN
                     NAKDHPGVRCWPSGIPEKNNIPDGLKVVQTPDVMIFLYESRTIYRQVFTDGRPLPKDA
                     QPTWMGYSVGRWEGDTFIVDTIGQNGKTWLDMRGLPGTESLRVIERYRRPTIGRITID
                     VTIDDPMAYTKPWDVQLAWRLVPDTDLIESICEENNRDLPHMVGK"
     gene            complement(4546..5598)
                     /locus_tag="AC16CY_13840"
     CDS             complement(4546..5598)
                     /locus_tag="AC16CY_13840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3354925.1"
                     /translation="MHGLYLLWWVEERGISPATVAALLAVGDLAAFLLEVPTGWVADR
                     LGHRASLIAGSSLQALGMLACWLGRGVPDLVIASLLVACGDAFRSGADQALLYRTCVA
                     LGREDRFQPTEARTRAAGLVALVVLLLGGGVMVTRWGFAAGWAAEVGLSLVGLAIACA
                     MIEPPSGGSRNDKRESTPVSTRLLRRAHAIAILVIPAALLGGAASASSFLAQTGGLAG
                     LLGVTVLVAAITLAESAGAATAGLVTGPASAGNGCWWRSARPSACWPSRSRPRSSWRP
                     SLCLFSLVLPSRFVPCCCNASRTTTPAPAWRRPRARATWCARRLLCCSLEGGAGPHAG
                     TSGPYQLIRELRAMRP"
     gene            complement(5697..6263)
                     /locus_tag="AC16CY_13845"
     CDS             complement(5697..6263)
                     /locus_tag="AC16CY_13845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIR3354926.1"
                     /translation="MSALKIKVIVGSTRANRFSEKPAQWIFDAARKRADFDVELLDLR
                     DYPLPLFEEPLPPGLAKDQYSNPLVIKWRDKIRESDGFIICTPEYNHGYPAVLKNALD
                     YTYASWGRKAVAFVSWGGAGGTRGVEQLRLVAIELDMVPTRWAVHIPNPWFVKDVSEI
                     DTEQNRQSAAALLDHLSWWAGALKAARK"
BASE COUNT         1137 a         2326 c         2346 g         1111 t
ORIGIN      
        1 gatcctgctc gagcatggcg cggatccgaa tcgcaccggc gccggcggca ccaccgcgtt
       61 gatgtgggcc gtccccgatc tcgggaaagt ccgcctgctt ctggcgcgcg gcgccaacgt
      121 gaacgccgcg tccgagacgg gacggacggc gctgctggtt gcggcgagct atccgcgaac
      181 ggtagaggtg ctccagctcc ttctcgagcg cggggcgaac ctccgcgcac aggatcgaac
      241 cggcgcgacg gcgctggggt ttgcggcccg ttccgccgac gtggacgtcg tccgttttct
      301 cgtcgcgcgc ggtctggatg cgcagtcgct gtctccgggg gcgcgccgcg cggcttttgc
      361 ccgcaacgat ctccgcacga gcgactatct gatcgcggcg gccccgatgc ccgcgcaaga
      421 tgatctgctg gtcgccgcca tctggcagcc cgccaggctc gtgtcccgct ggatcgagct
      481 gggcgcggat gtgaacgcga gcgcgccggc gcagtacgcg agaacgccct tgatgaacgc
      541 cgtgacgtcg gaggccgaag gcgccgacac cgtccgactg ctcctcgaga aaggcgccaa
      601 tccgaacgcc cagatgaccg agggggagag gccgctcgac tgggcgatct acaagggcga
      661 caaggcgaag atccaggcgc tcgagcaata cggcgcgacc aggggacagg gaccgaggca
      721 ggcagtcatt gctccacccg caacgggcgg cggggttgat gcgcgcacct ccctcgcacg
      781 gagcctgacc aggctcatgg aagtcgcgcc gaaattccgc gatcaggcca cgtgcatctc
      841 gtgccaccac aacacgatgc cggcgctcgc cgcggccgcg gcgcgaacga agggcgttcc
      901 cgttgacgag ccgcgcgctc gcaagaacgt cgaggacctt cgaacgttct ttgccggaaa
      961 cgtggcacgc atgatgctcg gcgatcccgc cgtcggtggt gaagcgctga cgaccggata
     1021 cgcccagatg gcgcttgccg ccgaaggcca tccgctcgac acggtgacgg cctcgatgac
     1081 gcactggctg atggccagac agatgcccaa cggcagctgg ctcgggaacg ggctcaaccg
     1141 gcctccttcc gaatacagcc tcgtcagcca cacggcgatc gccgctggcg ggctgaaggc
     1201 gtatcccttg cccggccgca gtggtgaggt cgccgagagt gtgcggcgcg cgggtcagtg
     1261 gcttctcgcg gcgaaggcaa cgtcggccga agagcgcggg atgcggctca tggggctcgt
     1321 ctggactggt gctcctcggt ctgccgtcga ggccgcaatc cgggagattc gcgagcggca
     1381 ggaggcgtcc ggaggctggt ctcagttcgg gaggacggag ccggacgcgt acgcgaccgg
     1441 cctatcgctc tacgcgctgc acgtcgccgg tgtgcgggcc acggacgaga cgtacaggaa
     1501 gggcgtggcg ttcctgctct cgacgcaata cccggatgga acgtggctgg tgaagacgca
     1561 ttccttccct gtgcagcgct acttcgaaag cggattcccc tacggcaggc atcaatggat
     1621 ttccaccgcg ggcaccagtt gggcaagcct tgcgatcgcg cacacactcc ctgacgccag
     1681 gaagtgatcc aaggaggatg agatggctgg aatccggatg ggaaaagtcg tcctgggcgg
     1741 cttggccgcc gggctcatca tcaacatcgg cgagacgatc ctgaacattc ctctgctcgg
     1801 cgccgagatg gagcaggcgc tgcaggcacg aaacctgccg gccgtcggcg ggggagcgat
     1861 cgcctatttc gtcggcgtct gcttcctgct cggcatcgtc atcgtctgga cctacgcggc
     1921 agtgcgcccg cggctcggac cagggccgaa gaccgcggtg atggtggccg ttctcgtctg
     1981 gctgctctcg ttcgtatggg ccggcggcgc gcaagcggcg atgggcatca tgccgctcaa
     2041 gctcaccgtc gtgggcctcg tctggggcct gggcgaactc gtgatcgcca cgctcgtcgg
     2101 cgcgaagctg tacaccgaaa cagcgtgaaa ggggtctggc cccttcacgc tacttcgccg
     2161 cggtgatctt ccagatacgg ttgccgctgt cgtcggacac gagcagcgcc ccgtcgcgag
     2221 ccaccgtcac acccacgggt cgtccccact gctcgatcgg cgtgccgttg gacccgtcgc
     2281 tggccaggaa gccggtcagg aagtcctcga tcggaccggg cttgccgttc accatcggga
     2341 agaacaccac cttgtagccc gaggccactg accggttcca cgatccgtgc aacgccacga
     2401 agccgccatt gcggaaccgc tgcggaaact gcgtgcccgt gtagaaggcg atgccgagcg
     2461 ccgcggcgtg agagggcagc agcacgtccg ggacggtcgc gcgcttcacg agatcgggga
     2521 aggatccgat gtatcgggga tcgtagttgc tcccgatgta ggaatacggc cacccgtaaa
     2581 acgcgccatc cttcaccgag gtcgcgtagt cgggcacgag atcgtcgcca aggttgtcgc
     2641 gctcgttgat cgcggtccag acgatgtcgg tccccggctg cagcgcaaga ccgaccgggt
     2701 tgcgaatgcc ggatgcgaac acacgatagc ctgtcccgtc cggattgaac tcgaggatcg
     2761 cggcgcgccg acagtcctcg cccgcgttgt tgttcgattg cgagccgacg gcgacgtaca
     2821 tcttggtgcc ggcacggttg aagatgatgt tgcgcgtgga gtggccgccc gcaggcaggt
     2881 cgagcagctt ctccggctgg ccttgcgcct gaaggtcgcc actcgagtac ttgtagcgaa
     2941 cgagcgacgc ggtatttccg acgtacagat acccgtcatg gaacgcgagg ccgaacggtg
     3001 ccgccagcgt gccgggcccg cgtacggcga actcaggcgg cggcgtgcac gccggcgcgt
     3061 ccgccccgag caccggcggc gctccgcggc ccccgcccgg cggcggcccc gctcctgccg
     3121 cgccacggcc gggggcgcca ccaggagcag cgccaccagg agcgccgcca gggcgagcgc
     3181 cgccctgggg agccgctcca cctccgcgcg gcgccggcgg ctcgccttgc gcgaacacgc
     3241 cctgcgcttc gaacacgccg tcgttgttgg cgtcgcgcag gacggtgatg gaattggctc
     3301 ctggcgagct gacgaagagg tcgccgttcg gcgcgtagac catcatgcgc ggtgcgcgca
     3361 actcggcgta gctcgtcacg gtgaacccgg ccggcactct tggcgtgatc ccttcgggtc
     3421 tgttcacgga tcggctcggc ttggggctcg cggcgccggg cgccggcagc gcctcggccc
     3481 gcggctgcgg gacgacgctg ttctgctgcg cgacgaggac gcccgcggcg agcgtgatgg
     3541 cgacgagtcc tgcgaacgcc ctgattgctg ttgtcatgct gtgctcccgt tactttccca
     3601 ccatgtgcgg caggtccctg ttgttctctt cgcagatgct ctcgatcagg tccgtatcgg
     3661 gcacgagcct ccaggccagc tggacgtccc agggcttcgt gtacgccatc gggtcgtcga
     3721 tcgtgacatc gatcgtaatc cgtccgatgg tcgggcgcct gtaacgttcg atcacccgga
     3781 gcgactcggt ccccggcagt ccccgcatgt cgagccacgt cttgccgttc tggccgatcg
     3841 tgtcgacgat gaacgtgtcg ccctcccagc gtccgacgga gtaccccatc cacgtgggct
     3901 gcgcgtcttt cggcagcggc cggccgtcgg tgaagacctg ccggtagatg gtccgcgact
     3961 cgtacagaaa gatcatgacg tccggcgtct gaacgacctt gagcccgtcc gggatgttgt
     4021 tcttttcggg aatgcccgat ggccagcacc ggacgccggg gtgatccttc gcgttgttct
     4081 cgatgcgttc cttgtagagc gcttcggccc aggggcgcag cggcacggaa ccgggcttca
     4141 ggtcggcggc caggttgagc agatgcttga cggcctcgac cggcatccag aagcccgaca
     4201 gatcgacctt gccgtcgggc gtccgccgtg gtgaggcgtt catgtccacc ttgccgtcgg
     4261 ccgtgcgcgg catgttcttc cacgggtacg gttcccactg cgcctcgccc gatgcggcag
     4321 tcgctgccac cagagcaatc gccaggagac acctcggaat gctcgtgcgg atctctatca
     4381 tctctactcc ctgtgtcggc gaattctact ctacagctcg cttgtgttga ggccattgtg
     4441 gcagaccgtc gcacgagaat ccgcctttcc agcgggaacg acgttgccaa aggcaccaac
     4501 acaagcgagt cctagggtta gtagacgcgt cgcgttcgcg ccggctcagg gtctcatggc
     4561 gcgtaactcc cgaatcagtt gatacggtcc actagtgcct gcgtgcggcc cggctccacc
     4621 ctccagcgag cagcagagca accgccgagc acaccatgtc gcacgcgctc gcggccgacg
     4681 ccacgcgggc gcgggcgtcg tcgtgcgcga ggcgttgcag cagcacggca cgaatcggct
     4741 gggcaacacc aacgagaaaa gacagagcga cggccgccat gaggagcgtg gacgggatcg
     4801 cgacggccag catgccgacg gccgtgccga acgccaccag catccattgc ccgcggaggc
     4861 cggccccgtg acgaggcccg ccgttgcggc gcccgctgat tccgccaggg tgatcgccgc
     4921 tacgagaacc gtgaccccca agagaccggc caatccgccg gtctgcgcga gaaacgacga
     4981 cgccgaagcg gcgccgccaa gcagcgccgc gggtatcacc aggatggcga tcgcgtgggc
     5041 cctcctaagc agcctcgtcg aaaccggcgt cgattcgcgc ttgtcgttcc ggctcccgcc
     5101 gctcggcggc tcgatcatcg cgcacgcgat cgcgaggccc acgagggaga gcccgacttc
     5161 ggcggcccat cccgccgcga agccccatcg cgtgaccatc acgccgccac cgaggaggag
     5221 cacgacgagg gccacgaggc cggctgcacg cgtgcgggct tcagtcggtt ggaagcgatc
     5281 ttcccggccg agggcgacgc acgtgcggta gagaagcgcc tgatcggcgc cagacctgaa
     5341 ggcgtcgccg cacgcgacga gcaggctcgc gatgacgaga tcgggaacgc cgcggcccag
     5401 ccagcatgca agcatgccga gagcctgtaa ggacgatccg gcgatcaggc tggcccgatg
     5461 gccgagccga tcggccaccc aaccggtggg cacttcgagc aggaaggccg cgaggtcgcc
     5521 gacggcgagc aacgcggcaa ccgtggccgg ggaaatcccg cgctcctcga cccaccacag
     5581 caggtagagg ccgtgcatca cgccctccac gctggcgacg tgatacggca ggtagagcgt
     5641 caggtttcgg gcgagcgatc aattaggagg catgggatca gcttaccgcg tctgcgctac
     5701 tttcgcgcag ccttcagcgc cccagcccac cacgacaggt gatcgaggag cgcggcggcg
     5761 ctctgacgat tctgctcggt gtcgatctcg ctgacgtctt tcacgaacca ggggttcgga
     5821 atgtgcacgg cccatcgcgt gggcaccatg tccagctcga tcgcgaccag ccggagctgc
     5881 tcgacgcccc tggtgccgcc cgcgccgccc cagctgacga acgccacagc cttgcgaccc
     5941 cacgaggcgt aggtgtaatc gagggcgttc ttcagcacgg cagggtagcc gtggttgtac
     6001 tccggcgtgc agatgatgaa gccgtcgctc tcgcggatct tgtcgcgcca cttgatgacc
     6061 agcgggttcg agtactggtc cttggccagc ccggggggca gcggttcctc gaacaggggc
     6121 agcgggtagt cgcgtaggtc gagcaattcg acgtcgaagt cggcgcgctt cctggcggcg
     6181 tcgaagatcc actgagccgg cttctcgctg aatcggttcg cacgggtgct gccgacaatc
     6241 actttgatct tgagtgcgct catgagccgt tcattctgcc acactctccc aactcccaac
     6301 gtgtttgttt agcgcgttcc gattgatcag gtcggccgat cgccgtgcca cggagacctg
     6361 ccggtccggg cgtcgccggc gctgcacgcc ggaaatcggt ttccgctccc gccctgccga
     6421 cacgtggcga gtagtcgcta cctcgcggcg acatccggca cgcggcacaa agcgcccgcg
     6481 acgaatcgcg tcaacgagaa cgcggggacg gaactcgccg cggaatcgtc gcttcacccg
     6541 atttcctgct tcgcagtggc ttggcgttgg cgcaggcgcc gccgacccgg caagcctccg
     6601 tggcactggc tgctcaggac tcgcctgccc ctcggcccgt cgacacgctg gatgtgtcct
     6661 ggagtccggc atgtcgcaag cccgcacacg cgatagacgg ccatcggcgt cgcaggcgta
     6721 cggagacgcg ggttgaggag cgagggcgtt agtcgatcgc gacgaaggag cggcgaccga
     6781 tcgtcaggca cgcgccgacg atgccgatgg ccgggcgccg cgacccgccg gcggatggac
     6841 acgccagcgt gtcgacgggc acggggctgt tggctcgcat gacagggact cggcacgcgc
     6901 taatcaagaa cctcccaact
//
LOCUS       DBNGSH010000598        10433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2437051, whole genome shotgun sequence.
ACCESSION   DBNGSH010000598 DBNGSH010000000
VERSION     DBNGSH010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2437051"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="AC16CY_13850"
     CDS             <1..551
                     /locus_tag="AC16CY_13850"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3354927.1"
                     /translation="RAGVHLVQIRERDLPDRALLALVTRAVEAARPTRTRVLVNDRVD
                     VAITSGAHGVHLRSDSMPASRVRAVAPPGFLVGRSVHSSGEAARLAEEGGLDYLMFGS
                     VFETPSKPGRPAAGVAGLAEAVAAARTLPVLAVGGITVARLGALSGSGCAGFAAIGMF
                     ADGAEDALAGTVAAALAPCENP"
     gene            558..1325
                     /locus_tag="AC16CY_13855"
     CDS             558..1325
                     /locus_tag="AC16CY_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354928.1"
                     /translation="MDAGFKSPLIDFFKRGDVARDVRLVAAQGALTPRVHEQLALLVM
                     LVADPDPEVAAAADATLRIIPRGSLAAFLARSDVSQDLLAFFARRGIEPSAQPLEDVD
                     APIIDTAQGPEIPDDEDTEQSALQRLAAMSVPQKMSRATKGTREERAILIRDPNKIVA
                     VAVMSSPKLTEAEVEAIAKMSSVSDEILRIVAHNRNWMKSYAIVSALVKNPKMPLALS
                     MNLINRLNDKDMRMLSTNRNVPEVLRTTARKKIVFDK"
     gene            complement(1345..1596)
                     /locus_tag="AC16CY_13860"
     CDS             complement(1345..1596)
                     /locus_tag="AC16CY_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354929.1"
                     /translation="MAKAPQGFVPLDALKSSPPAARDILADIRRIYFKTTRQTIEGDF
                     AHAIELLKRLESEREREKATVYMHGLAELRKEWGRKKRK"
     gene            complement(1598..3343)
                     /gene="polX"
                     /locus_tag="AC16CY_13865"
     CDS             complement(1598..3343)
                     /gene="polX"
                     /locus_tag="AC16CY_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR3354930.1"
                     /translation="MDNRPVAQVLTEIADLLEIKGENAFKIRAYRSAAETVATWPDAV
                     ARLDERHLRELPGIGKDLAARIRELVSTGSCQYHQQLLGEFPSTILDLLRLQGVGPRT
                     VALLHSALGIKSVDQLAAAAKAGRLRGLKGIGPKKEAQMLSSIEERAKDAGRHLLTDT
                     AATAAALVEYLHGRAPDVELVPVGSLRRGMDTCGDIDILAVPGPSRVEGGGEVTLMDA
                     LTTYPLVERVLGQGDTKTSVRLRGGYQADLRLVPHESRGAAMQYFTGSKAHNIAVRDL
                     AVQLGYKLNEYGLFRIDDESRVAGDTEEGIYQALGLAWIAPELRENRGEIDAARTGSL
                     PALVSASDLRGDLHMHTTATDGRDDLLTMAEAAHRMGHRYIAITDHSKALAMANGLDE
                     RRALEHAARIRALNGRFDGLTLLAGIECDILADGSLDLADDCLAELDVVIASVHSHFS
                     QEPQQLTDRVLRAMECPWVDIMGHPTGRLLLKRDPLRLDMAAVIDAAARHGVALEINC
                     LADRLDLNDVNARAARERGVPLVISTDAHSVGALSGLRWGVEVARRAWVGPGDLLNSL
                     PVETLRGRLRRSRRS"
     gene            complement(3336..4325)
                     /locus_tag="AC16CY_13870"
     CDS             complement(3336..4325)
                     /locus_tag="AC16CY_13870"
                     /inference="COORDINATES: protein motif:HMM:NF020133.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP family kinase ATP-binding protein"
                     /protein_id="HIR3354931.1"
                     /translation="MTIRTSAPTRIDLAGGTIDIWPLYLFHPGAQTLNAAISLRATAI
                     IEPRDDARLSIRSVDTGVTVEADDWRSLRASRDLRLLSLLVHFFEARGITLTTSSESP
                     AGAGLAGSSALNVAVCAALSEWKRAHYEPEALLQIAMNVEAQAIDVPTGLQDYRPALY
                     GGIAAVELDVDGVRRVPLDVDLAELQRRLVLCYTGEPRNSGTNNWEITKKHIDGDRHV
                     FDCFERIRDTAAAMRVSLEAADWDGVGRAIEQEWDNRKRLAPGVTTPVIDGLIAKARA
                     AGATAAKVCGAGGGGCLFCYGPPAARSAIQDALAAGGARVLDYSFERHGLTRG"
     gene            4466..5554
                     /gene="nagZ"
                     /locus_tag="AC16CY_13875"
     CDS             4466..5554
                     /gene="nagZ"
                     /locus_tag="AC16CY_13875"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR3354932.1"
                     /translation="MPSAIRRDIGQLLIGSLPGPTITPETRSLAREFSLGGLILFARN
                     IEAPEQVAELSLDTQELASELPLWVSVDQEGGRVARLKAPFTEWPPMAVLGRSGSPEL
                     AGRFAAALAAELRAVGITLDYAPVLDIHTNPKNPVIGDRALAEDAESVARLGAAIING
                     LQENGVAACGKHFPGHGDTSVDSHLALPVVEHPPDRIRRVECVPFREAIRAGVAFIMT
                     AHVLVPSLDEENPATLSPAIVGTLLRDELKFGGVILSDDLEMKAVANSYSVPEAAVRA
                     VAAGCDGLLVCSGNVDLQAAALEALVHAVEEGRISYKRLEDALARLRRAKERFLGAPV
                     SPRHTAKLRQVLGCDAHQRIVDEMARYL"
     gene            5560..6477
                     /locus_tag="AC16CY_13880"
     CDS             5560..6477
                     /locus_tag="AC16CY_13880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HIR3354933.1"
                     /translation="MRKPRALRPGDRMAIVAPASAFARDEFDRGVQEIERLGFVPVFD
                     DSVFARAAGYLAGTAEARAASFMKHWTDPDVAALVAVRGGFGSVHLLPLLDRARIAER
                     PKLFIGYSDNTSLLSWLTCQCGMTALHGPMLEGRLARGMYDRRSFVELTTGGSGLSLT
                     PEGVTVLRDGEASGPLFGGTITQLAASLGTPFAFDPPEGCVLFLEDVNERPYRIDRLL
                     TQLLLSGVLARARALVFGEMRGCEEPGGAASITEVISRITNACDGPVILGFPSGHTTG
                     PTWTLPLGVAVRVSTRPRPAIVVEDAPVE"
     gene            6470..7879
                     /gene="mpl"
                     /locus_tag="AC16CY_13885"
     CDS             6470..7879
                     /gene="mpl"
                     /locus_tag="AC16CY_13885"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR3354934.1"
                     /translation="MSDRKRIYLIGICGTAMATLAALLKSRGHDVRGSDQNVYPPMSD
                     FLIGQDIHALSGYAADHITGDIDLVVVGNAISRGNPELEEVLDRKIRYCSLPEAIREF
                     FLWGSRSIVLSGTHGKTTTTALTSWLLTSGGRDPSMLVGGIALNFGDEGSSYRVGAGR
                     DFVLEGDEYDSAFFDKTAKFLKYLPDVAVIGNVEFDHADIYANLDEVLLAFRRLVTLV
                     PRSGLLVLGADSPHAVALAKAAVSPVETFGLAGEATWQADAIEHRDGRTHFVVRRRGE
                     PYGRFESPLLGVHNVRNAMAAMAVGSYAGLGPSELAEGLRTFKGIKRRLEVVGVADEV
                     TVLDDFAHHPTAVHETLSALRTGYPDRRLWAVFEPRSASSCRRVFQDDFARAFGAADE
                     VILAPVFRSTLPEAERLDTGQLVEQLRARGQRARGVADNDDIIRAIVDEHRSGDVVVL
                     MSNGGFGGIHRKLLRALGA"
     gene            7876..9507
                     /gene="pxpB"
                     /locus_tag="AC16CY_13890"
     CDS             7876..9507
                     /gene="pxpB"
                     /locus_tag="AC16CY_13890"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HIR3354935.1"
                     /translation="MTGVRVREAGDSALLVELDAVIDPVINARAIAIAAAIDDERLAG
                     VRDVVPTYRSVALHFDPLVIGVEAVRAAVERALFARPRRAQTRVIEVPVVYGGDAGPD
                     LGEVAGRAGLAPDVVVQLHASETYRVFMLGFLPGFPYLASVDERIAAPRRAVPRLRVP
                     AGSVGIAGRQTGIYPMESPGGWNIIGRTGVRVFDPGRVPPALFQPGDSVRFVPSPTAD
                     VALGHAVSASPSAAVNTASRSVTVVRPGLFTTVQDGGRWGHQAHGVPVSGALDNVSHR
                     LANAALGNAPDAATLEVTLSGPEVRFDHDAKVAIAGADLQAAVDGARLPLGAAAECRA
                     GGVLQFGERRFGARAYVAFDGGVDVPPVLGSRATHVRAGLGGYHGRALAAGDRLALGP
                     PGGRQARAVRSPGISIAGGAKLRVLPGPQQDFFHESALDLLEGSRFVVTPNSDRMGYR
                     LSGARLPRIEGMEMISDAAFAGGIQVPASGEPILLMADRQTTGGYPQIAVVVTADLAR
                     AGQLAPGDWVEFVRCSRAEAIDALAAQEAMFGASR"
     gene            9494..>10433
                     /locus_tag="AC16CY_13895"
     CDS             9494..>10433
                     /locus_tag="AC16CY_13895"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3354936.1"
                     /translation="MPPGRTALSPREGVRLHAFSTIGVGGPARWFYTAERPDEIRSAH
                     RWCAEQNVPMFVLGGGSNVLVADQGFAGLVLHVGVMGVSMAADSGDTIVTAGAGEPWD
                     PLVKTLVERGLGGVECLSGIPGTVGGTPIQNVGAYGQEVASSIDRVVVFDRVRGEFGE
                     IATADCGFSYRMSRFKGTDAARFVVCQVVFRVRPGRPTVASPALVTDLSNRRIDTPGL
                     PDVRDAILAVRRRKGMVLDAHDPDTRSVGSFFMNPVVAASDRERNASASGERVPGFAA
                     GGDRVKIPAAWLIERSGFVKGHADGSVGLSTKHPLAI"
BASE COUNT         1558 a         3400 c         3698 g         1777 t
ORIGIN      
        1 caagggccgg tgtccacctg gtgcagatca gggagcgcga cctcccggat cgtgcgctgc
       61 tggcgctcgt gacacgggcg gtcgaagccg cgcggccaac ccggacgagg gtcctcgtca
      121 acgaccgcgt ggacgtcgcc attacctccg gcgcgcacgg cgtgcacctg cgatccgact
      181 ccatgcccgc gtctcgagtg cgggccgtgg cgcctcccgg gttcctcgtg ggaaggtcgg
      241 tgcactcatc cggcgaagcc gcccgcctcg ctgaggaagg ggggctcgac tatctgatgt
      301 tcgggtcggt gttcgagacg ccgtcgaaac ccggacggcc cgcggcgggc gttgccggcc
      361 tcgcggaggc ggtcgccgcg gcgcgaacgc tgcccgtgct cgccgttggc ggaatcacgg
      421 tggcacggtt gggcgcgctg tcgggatccg ggtgcgccgg ctttgcggcg atcggcatgt
      481 tcgccgacgg cgccgaagat gcgctggccg ggacggtcgc cgccgcgctg gcaccgtgcg
      541 aaaatccgta atcactcatg gatgccggtt tcaagtcgcc gctgatcgat ttcttcaagc
      601 ggggcgacgt cgctcgcgac gtgcgcctgg tcgcggccca gggcgcgctg actccgcggg
      661 tgcacgagca actggccctg ctcgtgatgc tcgtcgctga cccggacccc gaggtggccg
      721 cggccgccga cgccacgctg cggatcattc cgcgcgggtc gctcgcggcg tttctcgccc
      781 gctcggacgt gtcgcaggac ctgctggcgt tttttgcccg ccgcggcatc gagccgtcgg
      841 cgcagccgct cgaggacgtc gacgcaccga tcatcgacac ggctcagggc cccgagatcc
      901 cggacgacga ggacacggaa cagtctgcgc ttcagcggct cgcagcgatg agcgttccgc
      961 agaagatgtc cagggccacg aagggcacga gggaagagcg ggcgattctc atccgcgatc
     1021 ccaacaagat cgtcgccgtc gccgtgatga gcagtcccaa gttgaccgag gcggaggtcg
     1081 aggccatcgc gaagatgtcg agcgtgtccg acgaaatcct tcgaatcgtg gcccacaacc
     1141 gcaactggat gaagagctac gcgatcgtgt cggcgctggt gaagaacccg aagatgcccc
     1201 tggcgctgtc gatgaacctg atcaaccggc tgaacgacaa ggacatgagg atgctgtcga
     1261 ccaatcgcaa cgtgcccgag gtccttcgca cgaccgcgag gaaaaagatc gtgttcgaca
     1321 agtgacagct gcctggggcc gcagctattt ccgtttcttc cgcccccact cctttcgcaa
     1381 ctcggcgagc ccgtgcatgt agacggtcgc cttctccctt tcccgctcgc tctcgaggcg
     1441 cttcagcagc tcgatggcgt gggcgaagtc gccctcgatg gtctgccggg tcgtcttgaa
     1501 gtagatgcgc ctgatgtcgg cgaggatgtc gcgggcggcc ggagggctgc tcttgagcgc
     1561 gtcgaggggc acgaagccct ggggagcctt cgccacctca gctcctccgg ctccgccgca
     1621 gcctgccgcg cagcgtttcg accggcagcg aattcaggag gtctcccggc ccgacccatg
     1681 cgcggcgcgc cacctcgacg ccccatcgaa ggccgctcag ggcgccgacc gagtgtgcgt
     1741 ccgtcgaaat cacgagcggg actccgcgtt cccgggcggc ccgggcgttc acgtcgttca
     1801 ggtccaggcg gtcggccagg cagttgatct cgagggccac accgtgcctg gcggcggcgt
     1861 cgatcacggc cgccatgtcg agacggaggg ggtcgcgctt caacaacagc cggcccgtgg
     1921 ggtgtcccat gatgtccacc catgggcact ccatcgcgcg aagcacgcgg tcggtcagct
     1981 gctgcggctc ctgcgaaaag tgagagtgca ccgatgcgat cacgacatcg agctcggcga
     2041 ggcagtcgtc cgcgagatcc aggctcccgt cggcgaggat gtcgcactcg atgccggcga
     2101 ggagcgtgag gccgtcgaag cggccgttga gcgcccggat gcgggcggcg tgctcgagcg
     2161 cccggcgctc gtcgaggcca ttggccatcg cgagcgcttt gctgtggtcg gtgatggcga
     2221 tgtatcggtg acccattcgg tgggcggcct ctgccatcgt gagcagatcg tcgcgcccgt
     2281 cggtggcggt ggtgtgcatg tggaggtcgc cgcgaaggtc ggaggccgag accagcgcag
     2341 gcagcgaccc ggtccgtgcg gcgtcgatct cgccgcgatt ctctcggagt tcgggtgcga
     2401 tccacgccag gccgagggcc tgatagatcc cttcctctgt gtctccggca acgcggctct
     2461 cgtcgtcgat ccggaagagt ccgtattcgt tcagtttgta gccgagctgt actgcgagat
     2521 cccgtacagc gatgttgtgc gctttcgagc cagtgaagta ctgcatcgcg gcgccccggc
     2581 tctcatgggg aacgagacgg aggtcggcct gatagccgcc acggagccgc acgctcgtct
     2641 tggtatcgcc ctggccgagc actcgttcga cgagaggata ggtcgtcaat gcgtccatca
     2701 gcgtcacctc gccgccgccc tcgacgcgac tcgggccagg gacggcgagg atgtcgatgt
     2761 cgccgcaggt gtccatgccg cggcgaaggc tgccgacggg gacgagctcg acatccggcg
     2821 cgcgcccgtg aaggtactcg acgagcgctg cagcggtggc ggccgtatcg gtcagcagat
     2881 ggcgccccgc gtccttggca cgctcctcga tggacgagag catctgcgct tccttcttcg
     2941 ggccgatacc tttcaatccg cggaggcgcc cggctttcgc ggccgcggca agctggtcca
     3001 cgctcttgat gccaagggcg gagtgaagca gggcgacggt cctcggtccg acaccctgca
     3061 ggcgcagcag atcgaggatg gtcgagggga attcaccgag cagctgctgg tggtactggc
     3121 acgagccggt cgaaacgagc tcgcgaatcc gcgcggccag gtccttgccg atccccggca
     3181 actcccgcag atgcctctcg tcgagtctgg cgaccgcgtc gggccaggtg gcgacggtct
     3241 ccgcggccga tcggtacgcg cgaatcttga atgcgttctc gcccttgatc tccagcaggt
     3301 cggcgatctc ggtgagcacc tgggcgaccg gccgattatc cacgcgtcag tccgtgacgc
     3361 tcgaacgagt agtcgagcac gcgggcgccg ccggcggcga gcgcgtcctg gatggcgctc
     3421 cgcgcggccg gggggccgta acagaaaagg cacccgccgc ccccggcgcc gcacactttt
     3481 gcggcggtgg ctcctgccgc tcgggccttc gcgatcagcc cgtcgatgac gggcgtcgtc
     3541 acgccgggag ccagccgctt gcggttgtcc cattcctgct cgatcgcgcg gcccacgccg
     3601 tcccagtctg cggcctcgag cgacacccgc attgcggccg ccgtgtcgcg gatccgctcg
     3661 aagcagtcga agacgtggcg gtcgccatcg atgtgcttct tcgtgatctc ccagttattg
     3721 gtgccggagt tgcggggctc tcccgtgtag cagaggacca gacgccgctg cagctcggcg
     3781 aggtccacgt cgagaggcac gcggcgaacc ccgtccacgt ccagctcgac ggccgcgatg
     3841 ccgccgtaga gcgcgggacg atagtcctgc agcccggtgg gaacgtcgat ggcctgtgcc
     3901 tcgacgttca tcgcaatctg aaggagcgcc tccggctcgt agtgcgcccg cttccattcc
     3961 gacagcgcgg cgcagacggc cacgttcagc gcggacgatc cggcgaggcc ggcgccggcc
     4021 ggcgattcgg aggacgtcgt gagcgtgatg cctcgcgcct cgaagaagtg gaccagcaga
     4081 gagagcagcc ggaggtcccg ggaggcgcga aggctccgcc agtcgtcggc ctcgacagtc
     4141 acgcccgtat cgacagaccg gatcgagagg cgcgcgtcgt cgcgcggctc gatgatcgcc
     4201 gtcgcgcgaa ggctgatggc ggcattgagc gtttgcgcgc ccgggtgaaa gaggtagagc
     4261 ggccagatgt cgatcgttcc cccggccaga tcgatgcgcg ttggcgcgga agtccggatg
     4321 gtcacggctg gattatagaa tttcacggac gttaaccgca gaggcacaga gaacacagag
     4381 ctctgtgtcc gccgcgtctt ctgtggttag cgtccgtcgt ctctaagact gagaagcgtg
     4441 aacatacggt tctataatgt tcgtcgtgcc gagcgcgatc cgtcgcgaca ttgggcagct
     4501 gctcatcggc agtcttcccg gccccacgat cactcccgag actcggtcgc tcgcgcgcga
     4561 gttttccctc ggcggcctga tcctcttcgc ccgcaacatc gaggcgccgg agcaggtggc
     4621 cgagctgtcg ctcgatactc aggagcttgc atcggaactg ccgctctggg tgagcgtgga
     4681 ccaggaaggc gggcgagtcg ccaggctgaa ggcgcccttt accgaatggc cgccgatggc
     4741 ggtcctggga aggagcggca gtccggagct tgccggccgc tttgcggcgg cgctcgccgc
     4801 cgagctgaga gccgtgggca tcacgctcga ctacgcgccg gtcctcgata tccacacgaa
     4861 cccgaagaac cccgtgatcg gcgaccgggc gctggccgag gacgccgaaa gcgtggcccg
     4921 cctcggcgcc gcgatcatca acggcctgca ggagaacggc gtggcggcct gcgggaagca
     4981 ctttcctggt cacggcgaca cgtctgtcga ttctcatctg gcgctgcctg tcgtcgagca
     5041 cccgccggat cggattcggc gcgtggagtg cgtcccgttt cgagaggcga ttcgcgcggg
     5101 tgtggcgttc atcatgacgg cgcacgtgct cgtcccgtcg ttggacgagg agaaccccgc
     5161 gacgctgtcg ccggcgatcg tcgggacgct gctgcgtgac gagctgaagt tcggcggcgt
     5221 gatcctgagc gacgacctcg agatgaaagc ggttgccaac agctattccg tgccggaggc
     5281 cgccgtgcgt gccgtggccg ctggctgcga tggcctgctc gtgtgcagcg gcaacgtgga
     5341 cctgcaggcg gcggcgctcg aagcgctcgt gcacgccgtg gaggagggcc gtatctccta
     5401 caaacgtctc gaagatgcac tggcacggct tcggagggcc aaggagcggt ttctcggcgc
     5461 acccgtcagc ccgcggcaca cggcgaagct tcgtcaggta cttggctgcg acgcgcacca
     5521 gcgcatcgtg gacgagatgg cgcggtatct ctgacgcgcg tgcgcaagcc acgagcactc
     5581 aggcccggcg accggatggc catcgtggcc ccggcgagcg cgtttgcgcg tgacgagttc
     5641 gaccgcggcg tccaggagat cgagcgcctc ggattcgtgc cggtgttcga cgattccgtg
     5701 ttcgccagag ccgctgggta cctggcggga accgccgagg ctcgtgcggc ttctttcatg
     5761 aagcactgga ccgatcctga cgtcgccgcg ctcgtggccg ttcgcggcgg tttcggcagc
     5821 gtgcacctcc tgcccctgct cgatcgcgcg cgcatcgccg agcggccaaa gctgttcatc
     5881 ggctacagcg acaacacctc gttgctctcc tggctgacgt gccagtgtgg catgacggcg
     5941 ttgcacgggc cgatgctcga gggcagactg gcgcgcggga tgtacgaccg gcgctcgttc
     6001 gtcgagctga cgaccggcgg gagcggcctg tcgctgacgc cggagggtgt gacggtgctg
     6061 cgcgacgggg aggccagcgg cccgctgttc ggcggcacga tcacgcagct cgcggcgtcg
     6121 ctcgggacgc cgttcgcctt cgacccgccc gaggggtgcg tgctgttcct ggaggatgtc
     6181 aacgagcggc cgtaccgcat cgatcgcctg ctgacgcagc ttcttctgag cggcgtgctg
     6241 gcgcgcgcac gcgcgctggt gttcggcgag atgcgcggat gcgaagaacc cggcggcgcc
     6301 gcctccatca cggaagtcat ttcgaggatc acgaacgcgt gcgacggtcc ggtgattctc
     6361 ggtttcccct ccggccacac gacgggcccc acctggacgc tgccgctcgg cgtcgccgtg
     6421 cgcgtctcca ccaggccacg gccggccatc gttgtcgagg acgcacccgt tgagtgaccg
     6481 caaacgcatc tacctgatcg gcatctgtgg cacggccatg gccacgcttg cggccttgct
     6541 caagagccgg gggcacgacg tacggggatc agatcaaaac gtctaccccc cgatgagcga
     6601 cttcctgatc ggccaggata ttcacgcact ctcgggatac gcggcggacc atatcaccgg
     6661 ggacatcgac ctggtcgtcg tgggcaatgc catctccagg ggcaacccgg agctggagga
     6721 ggtgctcgat cggaagatcc ggtactgctc tctgcccgag gccatccgcg agttcttcct
     6781 gtggggatcg cgctcgatcg tcctgagcgg cacgcacgga aagacgacga ccacggcgtt
     6841 gacgagttgg ctgctgacga gcggtggccg cgacccgtcg atgctcgtgg gcggcatcgc
     6901 cctgaatttc ggggacgagg gctcgagcta ccgcgtgggg gcggggcgcg atttcgtcct
     6961 cgaaggcgac gagtacgaca gcgcgttctt cgacaagacg gcgaagtttc tcaagtacct
     7021 gcccgatgtc gccgtcatcg gaaacgtcga gttcgaccat gcggacatct acgccaacct
     7081 cgacgaggtg ctcctggcgt tccgccgtct cgtgactctc gtcccccgta gcggcctgct
     7141 cgtcctcggg gccgacagtc cacatgctgt cgcgctcgca aaggccgcgg tgagcccggt
     7201 cgagaccttc gggctcgccg gcgaggccac gtggcaggcc gacgcgatcg aacatcgcga
     7261 tgggaggacg cacttcgtgg tgcgacggag aggggagccc tacggccgct tcgagtcgcc
     7321 gctcctcggc gtgcacaacg tgcggaatgc gatggccgcc atggccgtgg ggtcctacgc
     7381 gggccttgga ccctcggagc tcgccgaggg actgcggacg ttcaagggca tcaagcgcag
     7441 gctcgaggtc gtcggcgtgg ccgacgaagt gaccgtcctg gacgatttcg cgcaccaccc
     7501 gaccgcggtg cacgagacct tgtcggcgct gcgaacgggg tatccggatc gacggctgtg
     7561 ggccgtgttc gagccgcgat cggcctcgtc gtgccggcgc gtgtttcagg acgacttcgc
     7621 gcgtgccttc ggcgcggcag acgaggtgat cctggcgcca gtgtttcgat cgaccctgcc
     7681 cgaggccgag cggctcgaca ccgggcaact ggtggagcag ttgcgcgccc gtggacagcg
     7741 cgcccgcggc gtcgctgaca acgatgacat catccgcgcg atcgtcgacg aacacaggtc
     7801 cggcgacgtt gtcgtgctga tgtcgaatgg cggcttcggg gggatccacc gcaagctgct
     7861 gcgggcgctc ggcgcgtgac cggagtgcgc gtccgcgagg caggcgattc ggccttgctc
     7921 gtcgaactgg atgcggtcat cgaccccgtg atcaacgcgc gcgcgatagc catcgcggcg
     7981 gcgatcgacg atgagcgtct ggccggcgtg cgcgacgtgg tgccgacgta tcgctccgtg
     8041 gcgctccact tcgatccgct ggtgatcggt gtcgaggcgg tgcgggctgc ggtcgagcgc
     8101 gctctctttg cgcgaccacg gcgggcgcag acgcgcgtga tcgaagtgcc tgtcgtgtac
     8161 gggggcgatg ccgggccgga tctgggcgag gtcgcaggcc gggccggcct cgcaccagac
     8221 gtggtcgtgc agctccacgc gagcgagacg taccgagtgt tcatgctcgg gttcctgccg
     8281 ggtttcccgt atctggcgtc ggttgacgag cgcatcgccg caccgcgccg cgctgtgccg
     8341 cgtctgcgcg tacctgcggg ttcggtcgga atcgccggtc ggcagaccgg catctatccc
     8401 atggagtcgc ccggcggctg gaacatcatc ggacgcaccg gtgtgcgcgt gttcgatccc
     8461 ggcagggttc cacccgcgct cttccagccg ggcgattcag tgcgcttcgt cccgtcgccg
     8521 acggctgacg tggcgctcgg ccacgcggtt tcggcatcgc catccgccgc cgtgaacacc
     8581 gcgtcccgct cggtgacagt cgtgcggccg ggcctcttca cgactgtcca ggacggaggc
     8641 cgatggggac accaggcgca tggcgttccc gtgagcggcg cgctcgacaa cgtatcgcat
     8701 cgccttgcga acgccgccct cggaaacgct cccgatgccg cgacactcga ggtaacgctg
     8761 tcaggtcccg aggtgcgatt cgaccacgac gcgaaagtcg caatagccgg tgcggatctg
     8821 caggcggccg tcgacggggc tcgattgcca ctgggagccg cggcagagtg ccgtgccggc
     8881 ggcgtgcttc aatttggcga acgccgcttc ggggcgagag cctatgtggc cttcgacggc
     8941 ggcgtcgatg tgcctcctgt gctcgggagc cgggcgacgc acgtgcgcgc cggcctgggc
     9001 ggttaccacg gtcgagcgct cgcggccggc gatcggctcg cgctcggtcc cccgggcggg
     9061 cgccaggctc gcgcggttcg gtcgcctggt atctcgatcg ccggcggtgc gaagcttcga
     9121 gtgctgcccg ggcctcagca ggacttcttc cacgagtcgg cgctggatct gctggaagga
     9181 tcgcggttcg tcgtcacacc gaactcggat cgcatggggt accgtttgtc gggtgccagg
     9241 cttccgagga tcgaagggat ggagatgatc tcggatgcgg cgtttgccgg cggcatccag
     9301 gtgcccgcct ccggcgagcc gatcctgctg atggccgatc gccagacgac gggcggatat
     9361 ccgcagatcg cagtggtggt gacagccgac ctggcgaggg cagggcagct ggctccggga
     9421 gactgggtcg agttcgtgcg ctgttcacga gccgaagcga tcgatgcgct ggcggcgcag
     9481 gaggccatgt tcggtgcctc ccggtaggac cgcgctgagc ccgcgcgagg gcgttcggct
     9541 tcacgccttc agcacgatag gcgttggcgg acccgctcgc tggttctaca cggccgaacg
     9601 accggacgag attcggtcgg cgcatcgatg gtgcgccgag cagaacgtcc cgatgttcgt
     9661 gctcggcgga ggcagtaacg tactggtcgc ggaccagggc ttcgccgggc tcgtgttgca
     9721 cgtgggagtc atgggagtgt cgatggcggc cgactctggc gacacgatcg tgaccgcggg
     9781 ggccggtgag ccgtgggacc ccctggtcaa gaccctggtc gagcggggcc tgggcggcgt
     9841 cgaatgtctt tcggggatac cggggacggt gggaggcacg ccgatacaga atgtcggcgc
     9901 atacgggcag gaggtcgcga gctccatcga tcgcgtcgtc gtattcgacc gcgtcagggg
     9961 cgagttcggc gagattgcaa cagcggactg cggtttttcg tatcgcatga gccgcttcaa
    10021 ggggaccgac gccgctcgct tcgtcgtatg tcaggtggtg tttcgcgtgc ggcccgggcg
    10081 gccgacagtg gcctctccgg ccctggtaac cgacctctcg aaccggcgga tcgacacacc
    10141 tggcttgccg gacgttcgcg atgcgattct ggcggtacgg cgtcgaaagg gtatggtgct
    10201 cgacgcgcac gaccccgaca cgcgcagcgt gggatcgttc ttcatgaacc ctgtcgtggc
    10261 agcctccgat cgcgaacgga acgcgtccgc gtccggcgag cgcgtcccgg gctttgctgc
    10321 cggaggggat cgcgtgaaga taccggctgc ctggctcatc gagcgatcgg gtttcgtcaa
    10381 gggtcacgct gacggttcgg ttggtctctc gacgaagcat ccgctcgcga tcg
//
LOCUS       DBNGSH010000599         4163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4474494, whole genome shotgun sequence.
ACCESSION   DBNGSH010000599 DBNGSH010000000
VERSION     DBNGSH010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4474494"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="AC16CY_13900"
     CDS             <1..1025
                     /locus_tag="AC16CY_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354937.1"
                     /translation="AGQMWTKFAGVEGQIFPPRMASYVAVKLYGPGHVVAYNYIADFH
                     DGINVETYGNPDGSFASGPGIIDGPKYPTREYWDRRPVAIDFYNNYITNSHDNPIEAD
                     GSMHNIRIMRNMLINHPSHALCNQPTLGGPVYWVRNIAYNLPAGSTRLTNGAAGVLFY
                     NNTILSETTVAAASNIHWRNNLMLGQNAAPAILSVTTFTSYTSSDYNGFRVNSGAQAS
                     FEWSSPPPGVTADFTGPGRTATLETRRFATMADYAAATGQDRHSVAVDYDVFMNVRRL
                     DAQDAASVQKLYKAADFDFRLKAGSAAVDRGLMLPTVTDGYLGKAPDLGAVEAGHPLP
                     HYGPRR"
     gene            complement(1099..2823)
                     /locus_tag="AC16CY_13905"
     CDS             complement(1099..2823)
                     /locus_tag="AC16CY_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354938.1"
                     /translation="MLAGLVVSVAPLVLLIANRDWFFTPEGFLDPWQYVGFFRFYEDL
                     DYSPEEYKLARLPWILAGYGITRSMPTMPAAYLLHAIFLCALPLTFFGATYSMLRKTS
                     LAAILALCLGFYTHAHGSGGWDYHNTASGPLFLGTLWVTVLPSSRRGAPGALMLAGVM
                     VALTLHTNITLVNFLPALFYLHLVASRLENHQWPSRSMLLGRIGWSVVGALLATTVLG
                     TINWIVGRDFLFFESLLRKVTRFLTGSPDISGLPLLGAWVLGTGYLGLLAATMVSGIG
                     FLALERRKTGDDREMPRALSRQFLGAALLWIVGQFLGQPVLDWDTMAHPLIASCFLAL
                     AGLLSRGWPESVERRPALVIVLTALVLTSCLVATLPPVSTVAALAAPGIGLVAAVVFV
                     APIAVYLWRPSAATVCLFVAVFALGNRLVAGSPQNYAADDRCKVQPAVYQAIVDATSR
                     LVSIDPMYYRVRIWFGENEVIYPAPQCGVSLGWMGYSIRSMSSMNYLTKPHPMPEVDD
                     VPEDSIRQMRDSDAILTIVTDRPESVRTWEHRLAALGLDHREMYRFRIPVMDSGFTVY
                     AWAVTSSR"
     gene            complement(2899..>4163)
                     /locus_tag="AC16CY_13910"
     CDS             complement(2899..>4163)
                     /locus_tag="AC16CY_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thiol-disulfide isomerase"
                     /protein_id="HIR3354939.1"
                     /translation="GTITLAAAVGAGLRAQSAQPVPGAPTFTRDIAPILYANCVECHR
                     AGEIAPMSLITYAEVRPWARAIERRVVDGTMPPWHADAAHGTFRNERQLTLAQKDTVQ
                     RWAAAGAPEGDAAHLPAPPAFAAGWRIGAPDVILEMQEDYAVPASGTVLYEYFYIPTN
                     FTETKWVRAIEARPGNRSLVHHILVFHEAPSDGSNAAPAIQPNREDSRTPPPPPQGNR
                     PSRRSNVPSQLIATYAPGTNPQEFPAGTALRLPPGGTLRLQMHYTANGTAGTDRSRIG
                     LIFAKEPPAEEIRVSHFLNGQLVIPAGASDHQVSTDVTFVQDASLWGLFPHTHVRGKR
                     WNYLLELPDGSTQPLLAVPKYDFNWQTYYVYREPLRIPKGAKIRSTAWYDNSAANRSN
                     PDPTIDVRWGDQTWEEMQYTGIMYSVKR"
BASE COUNT          815 a         1311 c         1375 g          662 t
ORIGIN      
        1 gggcgggcca gatgtggacg aagttcgcgg gagtcgaggg tcagatcttt ccgccccgca
       61 tggcctcgta cgtcgccgtc aagctgtacg gccccggcca cgtcgtcgcc tacaactaca
      121 tcgccgattt tcacgacggc atcaacgtgg agacctacgg caaccccgat ggctcctttg
      181 cgtccgggcc gggcatcatc gacggcccga agtacccgac gcgcgagtac tgggaccgca
      241 ggccggtggc gatcgacttc tataacaact acatcaccaa ctcgcacgac aacccgatcg
      301 aagccgacgg cagcatgcac aacatccgaa tcatgcggaa catgctgatc aaccacccgt
      361 cgcacgcctt gtgcaatcag ccgacgctcg gaggcccggt gtactgggtc aggaacatcg
      421 cgtacaacct gccggcgggc tcaacgcgcc tgaccaacgg cgcggccggg gtgctgttct
      481 acaacaacac gatcctgtcg gagacgacgg tcgccgcggc gtcgaacatt cattggcgga
      541 acaacctgat gctcggccag aacgctgcgc cagcgatcct gagcgtgacg acgttcacga
      601 gctatacctc gtccgactac aacggcttcc gcgtgaactc cggtgcgcag gcgtcgttcg
      661 agtggagctc gccaccaccg ggcgtcacgg cggatttcac gggcccgggg cggacggcca
      721 cgctcgagac gcggagattt gccacgatgg ccgactacgc cgccgcgacc ggacaggatc
      781 ggcacagcgt cgccgttgat tacgacgtgt tcatgaacgt ccgccggctc gacgcgcagg
      841 acgccgcgag cgtccaaaag ctgtacaagg ctgccgattt cgatttcagg ctcaaggctg
      901 gatcggccgc cgtcgatcgc gggttgatgc tgccgacggt gacggacgga tacctcggga
      961 aggcgccgga cctcggtgcg gtcgaagcgg gccacccgct gccgcattac ggcccgcggc
     1021 ggtgatagcg cgcgaagatc gtccagatcg cgtcaggagt gcccggtgtc cgaccacgtc
     1081 gacgatcgga tgcgatgatc agcgcgagga ggtcacggcc cacgcgtaca cggtgaagcc
     1141 cgaatccatg acagggattc ggaagcgata catctcgcga tgatcaagcc ccagagccgc
     1201 gaggcgatgc tcccacgtcc gcaccgattc cggccgatcc gtgacgatcg tcaggatggc
     1261 gtctgagtcc cgcatctggc gaatcgagtc ctccggcaca tcgtccacct ccggcatggg
     1321 atggggcttc gtcagatagt tcatcgagga catcgaccgg atcgaatatc ccatccagcc
     1381 caggctcacc ccacactggg gcgctgggta gatcacctcg ttctcaccga accagatccg
     1441 cacgcggtag tacatcgggt cgatcgagac caggcgtgag gtcgcgtcga caatggcttg
     1501 ataaacggcc ggctggactt tgcagcgatc gtcggcggcg tagttctgag ggctgccggc
     1561 gacgagccga ttcccgagag cgaagacggc gacgaacagg cacaccgtcg cagcagacgg
     1621 acgccagaga tacacggcaa ttggcgcgac gaacacaact gctgcgacca acccaatgcc
     1681 tggcgcggcg agcgccgcga ccgtcgatac gggtggtaac gttgcgacca gacagctcgt
     1741 cagaaccaag gcggtgagca cgatcaccag cgctggccgc ctctcgaccg attctggcca
     1801 tcctcgcgaa agcagtccgg caagcgcgag aaagcagctg gcgataagcg ggtgggccat
     1861 ggtgtcccag tcgagaacag gctgcccgag gaactgcccc acaatccaca acagtgcggc
     1921 accgagaaac tgcctgctga gggcgcgggg catttctcgg tcgtcacccg ttttgcgtcg
     1981 ctcgagagcg aggaagccaa tacccgacac catggtcgcc gccagtagcc ccagatagcc
     2041 cgtgcccaaa acccacgccc ctaacaaggg cagaccggag atgtcagggg agccagtgag
     2101 gaagcgtgtg actttgcgga gcagactctc gaagaataga aaatccctgc cgacaatcca
     2161 gttgatcgtg ccgagaacgg tcgtcgccag gagggcgccg acgacgctcc agccgatgcg
     2221 cccgagcaac atggagcggc tcggccactg gtgattctcc agccgtgatg ctacgagatg
     2281 caggtagaac aaggcgggaa ggaagttgac cagcgtgatg ttcgtgtgaa gcgtcaacgc
     2341 gaccatgact ccggcgagca tgagggcgcc aggagcgcca cgccgggatg acgggagcac
     2401 cgtaacccag agcgtcccga gaaacagggg tcctgaggcc gtattgtggt aatcccaccc
     2461 gccagaacca tgcgcgtgcg tataaaaccc aaggcacaaa gcgagtatcg ctgcgaggct
     2521 ggttttccgc agcattgagt aggtcgcgcc aaagaacgtc agcggcagcg cacagaggaa
     2581 gatcgcgtgc agcagataag ctgcgggcat cgttggcatc gatctggtga tgccgtatcc
     2641 ggcaagtatc cacggcagac gcgcaagctt gtactcttct ggcgagtaat cgaggtcctc
     2701 atagaagcgg aaaaagccga cgtactgcca gggatcgaga aagccctccg gcgtgaagaa
     2761 ccagtccctg ttcgcgatca gaaggacgag tggtgcgact gacaccacga ggcctgccag
     2821 cacaacgtga acggaaggag gaagccgtcg gatcacggag acacctgccg cgggcgaaac
     2881 cccgcggcac tcggactgtc accgcttcac ggagtacatg atccccgtgt actgcatctc
     2941 ctcccacgtc tgatcgcccc acctcacgtc gatcgtcggg tcggggttgg atcggttcgc
     3001 ggcggagttg tcgtaccagg ccgtcgaacg gatcttcgcg cccttcggga ttctgagcgg
     3061 ctcccggtag acgtaatagg tctgccagtt gaagtcgtac ttcggcacgg ccagcagcgg
     3121 ctgcgtgctg ccatcaggaa gctccagcag gtagttccag cgcttgccgc gcacatgcgt
     3181 gtgcgggaag aggccccaga ggctcgcatc ctggacgaac gtgacgtcgg tgctgacctg
     3241 gtgatcgctg gcaccggcgg gaatcaccag ttgtccgttc aggaaatgcg agacgcggat
     3301 ctcttctgcc ggcggctcct tcgcgaagat caatccgatc cgcgatcggt cggtccccgc
     3361 ggtgccgttg gcggtgtagt gcatctgcag gcgaagggtg ccgcctggag gcaggcgaag
     3421 cgcggtgcct gccgggaact cctggggatt cgtgccgggc gcgtaggtcg cgatcagctg
     3481 gctcggcacg ttcgaccgcc gcgacgggcg gttcccctgc ggcgggggcg gcggcgtgcg
     3541 gctgtcctcg cgatttggtt gaatcgccgg cgccgcgttc gagccgtcgg agggggcctc
     3601 gtggaagacc aggatgtgat ggacgagcga gcggttgccg ggccgcgctt cgatcgcccg
     3661 cacccacttc gtctcggtaa agttggtggg gatgtagaag tactcgtata gaaccgtacc
     3721 gctcgccgga accgcgtaat cctcctgcat ctccaggatc acatccggcg cgccgatgcg
     3781 ccacccggcc gcgaacgccg gcggagcagg gaggtgggcc gcgtcgcctt cgggcgctcc
     3841 ggccgcggcc catcgctgaa cggtgtcctt ctgagccagt gtcaactgcc gctcgttgcg
     3901 gaacgtcccg tgagccgcat cggcgtgcca cggcggcatc gtgccgtcca cgacgcggcg
     3961 ctcgatggcg cgtgcccacg ggcgcacttc tgcgtaggtg atgagcgaca tcggagcgat
     4021 ctcgccggcc cgatggcact ccacgcagtt ggcgtagagg atgggcgcga tgtcacgtgt
     4081 gaacgtcggg gcgccgggca cgggctgcgc cgactgcgcg cgaaggccgg cgccgacggc
     4141 ggcggccagg gtgattgtgc cgg
//
LOCUS       DBNGSH010000600         2461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1758171, whole genome shotgun sequence.
ACCESSION   DBNGSH010000600 DBNGSH010000000
VERSION     DBNGSH010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1758171"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="AC16CY_13915"
     CDS             <1..876
                     /locus_tag="AC16CY_13915"
                     /inference="COORDINATES: protein motif:HMM:NF019229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3354940.1"
                     /translation="YVGNDGMPNQLWINQKNGAFKDMAFINGAAVNGAGNAEASMGID
                     AGDFDNDGDEDIFVTNWLAQMNILYQNAGNGVFEDRKAASGLGPPSLAKTGFGTAWID
                     YDNDGWLDLIALNGGVAMIEAQARAKDPFPLKMLNQLYRNNGTGRFEDVSARGGDPFT
                     LMDVSRGGAFGDIDNDGDVDVAVGTTAGPARLFINNVGSRQHWIGLRLLGVAGRDMLG
                     ARVQVTRGSGPALWRRARSDGSYASANDPRVFVGLGASADPVTARVQWPDGTREEWTN
                     LSVDRYVTLKQGTGK"
     gene            873..>2461
                     /locus_tag="AC16CY_13920"
     CDS             873..>2461
                     /locus_tag="AC16CY_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3354941.1"
                     /translation="MKITVRLKPDTTTVPTCRGSADVVSGFSRTVTDVVSVFRRTIVC
                     LLVVAAACGTPDRSAPGAPEAAVLRDVVLPDLSRMDPAVQEQVRQRHASMRETIARPG
                     ASDAERGAAYGELGILLHAGEYFEAAEPAYLNAQALMPRDVRWPYYLAHLHKSRGKTA
                     ESMAAFTRALELQPDDLPTLVWLGRAYLDQGQADKAAPLFERARQQAPRVTAVLVGLG
                     QAALMQRDYARAVSTLEEALSVDPSAQSVHSPLAMAYRGLGDTAKAEAHLKQWKNTDV
                     LVPDPLRQQLDLSLQSGLSYELRGVRALEARDFAAAAGFFKQGVDITPGTTPLGRSLR
                     HKLATALYMTGDVPGAVKWFDETLRFAPDGGIDETAAKAHYSLGVLAASGGRGEEAIT
                     HLAAAVRFSPTYVEAHVALADALRRRGRVEQSLLPYGDALRLNPLAADARFGYGIALV
                     RLGRYRQARDWFEDARQRHPDRIDFLHALARLQAAAPEDGVRDGERALAIVEGLLKQN
                     KSFDLVETLAMALAEQGRFEEA"
BASE COUNT          408 a          809 c          874 g          370 t
ORIGIN      
        1 tacgtcggca acgacgggat gccgaatcag ctctggatca atcagaagaa cggcgccttc
       61 aaggacatgg cgttcatcaa cggcgccgcc gtcaacggcg cgggcaacgc cgaagccagc
      121 atggggatcg acgccggcga tttcgacaac gacggcgacg aggacatctt cgtcaccaac
      181 tggctggcgc agatgaacat cctctatcag aacgccggga acggcgtgtt cgaggatcgc
      241 aaggccgcgt ccggactcgg gccgccgagc ctggcgaaga ccggtttcgg caccgcctgg
      301 atcgactacg acaacgacgg ctggctggac ctcatcgcgc tgaacggcgg cgtggcgatg
      361 atcgaagcgc aggccagggc gaaggacccc ttcccgctca agatgctgaa ccagttgtac
      421 cgcaacaacg ggaccggccg cttcgaggac gtgtcggccc gcggcggcga cccgttcacg
      481 ctgatggacg tcagccgcgg gggcgccttc ggcgatatcg acaacgacgg ggacgtggac
      541 gtggccgtcg gcacgaccgc cggtcccgcg cgtctgttca tcaacaacgt gggcagccgt
      601 cagcattgga ttgggctgcg cctgctcgga gtcgccgggc gcgacatgct cggagcacgc
      661 gtgcaggtca cgcgcgggag cggtccggcg ttgtggcgcc gggcgcgctc cgacggcagc
      721 tacgcctcgg ccaacgatcc ccgcgtattc gtggggctcg gcgcgtcggc cgatccggtg
      781 acggcgcgcg tgcagtggcc ggatggcacg cgggaagagt ggacgaacct gagcgtcgat
      841 cgctatgtca cgctgaaaca ggggactggg aaatgaagat cacggtccgg ctgaagccgg
      901 acactacgac tgtcccgaca tgtcgtggct ctgcagacgt agtgtccggc ttcagccgga
      961 ccgtgacaga cgtggtgtcc gtcttcaggc ggaccatcgt gtgtcttctc gtcgttgccg
     1021 ccgcatgcgg aactccggat cgatccgcac ccggtgcacc agaggctgcg gtattgcgtg
     1081 acgtcgtgct gcccgatctg tcgcgcatgg atccggcggt gcaggagcag gtgcggcagc
     1141 gccatgcatc gatgcgcgag accatcgcga ggcccggcgc gagcgacgcg gaacgaggcg
     1201 ccgcgtacgg ggagctcggg atattgctgc acgccggcga gtatttcgag gcggccgagc
     1261 ctgcctatct gaacgcgcag gcgctgatgc cgcgggacgt gcgctggccg tattacctcg
     1321 cccatctgca caagagccgc ggcaagacgg cggagtcgat ggccgccttt acgagagccc
     1381 tcgagctgca gccggacgat ctgcccacgc tggtctggct cgggcgcgcg tacctcgatc
     1441 agggccaggc ggacaaggcg gcgcccctct tcgagcgcgc ccggcaacag gcgccgcgcg
     1501 tgacggctgt gctcgtcggc ctcgggcagg cggcactgat gcagcgggac tacgcgcgcg
     1561 ccgtgtcgac gctcgaggag gcgctgtcgg tcgatccgtc agcccagagc gttcattcgc
     1621 cgctggcgat ggcgtaccgc gggctgggcg acacggccaa agccgaagcg catctcaaac
     1681 aatggaagaa caccgacgtc ctcgtgccgg atccgctccg ccagcagctg gatctgtcgc
     1741 tgcagagcgg gctgtcctac gagctgcgcg gcgtgcgcgc gctcgaggcg cgcgacttcg
     1801 cggccgccgc cggattcttc aagcagggcg tggacatcac gccgggaacc actcctctcg
     1861 gacggtcgct gcggcacaag ctcgcaacgg cgctctatat gaccggcgac gtgcccggcg
     1921 ccgtgaagtg gttcgacgag acgctgcggt tcgcccccga cggcgggatc gacgaaacgg
     1981 cggccaaggc ccactacagc ctcggagtcc tcgcggcgtc gggcggccgc ggcgaggagg
     2041 cgatcacgca tctcgcggcg gcggtacgtt tcagccccac ctacgtcgag gcccacgtcg
     2101 cgctcgccga cgcgctgcga cgacgcggcc gggtcgagca gtcgctgttg ccgtatggcg
     2161 atgcgcttcg actcaaccct ttggcggcgg acgcgaggtt cggctacggc atcgcgctcg
     2221 tcaggctcgg gcgttaccgc caggctcgtg actggttcga ggacgccagg cagcggcacc
     2281 ctgatcgcat cgactttctc catgcgctgg cgcgcctgca ggcagccgct ccggaagacg
     2341 gcgtccgcga cggggagcgt gcgctggcca tcgtcgaggg gttgctcaaa cagaacaagt
     2401 cgttcgacct cgtcgagacg ctggccatgg cgctcgccga gcagggccgg ttcgaggaag
     2461 c
//
LOCUS       DBNGSH010000601         1639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3795659, whole genome shotgun sequence.
ACCESSION   DBNGSH010000601 DBNGSH010000000
VERSION     DBNGSH010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3795659"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="AC16CY_13925"
     CDS             <1..563
                     /locus_tag="AC16CY_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3354942.1"
                     /translation="AEAGSPDTAERMQRMLQSMLIERFKLRVHTETRQLPLYYLVPAR
                     GDGKLGPRLRKSAFDCAALRARRGPGGAPPADGSEPPCQVSFKVGDGSMTIAFQGESA
                     SELARFVIPERDRPVIDKTNLPGTFDGELTFAPEPLPGFPRLPGSENGVSVYTALQEQ
                     LGLRLEPDHGPVEIIVIDGAERPTEN"
     gene            691..1176
                     /locus_tag="AC16CY_13930"
     CDS             691..1176
                     /locus_tag="AC16CY_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3354943.1"
                     /translation="MTSTTPLAGFARLFERHYEMVFRAALRVTGNPADAEDVLQTVFL
                     RVLAQGGEIEDVALPAAYFRRAAVNAAVDVLRRRELHAESSYDDEAPQVAVQPPLLLK
                     ERLRRAIAVLDNEDASLFLLRHVEGLSIEELAGIFKIEKNNVAVRLHRIKHRLQTEMQ
                     R"
     gene            1202..>1639
                     /locus_tag="AC16CY_13935"
     CDS             1202..>1639
                     /locus_tag="AC16CY_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3354944.1"
                     /translation="MSDDRLERALQEMKEEDVDSGTIEAVRARVWEKVGAGSGTCAEF
                     RPDFHAYLASELGASRRLLVDDHLSRCPGCRARIAEMKGERTVVAMPRGLPRRSLREG
                     GSSSRTVQWGALAAAAALLFGVYLGRDAIDAMMAPGGARATVVS"
BASE COUNT          271 a          540 c          562 g          266 t
ORIGIN      
        1 cggccgaagc agggtcaccc gacacggcgg aacgcatgca gcggatgctc cagtcgatgt
       61 tgatcgagcg cttcaagctg cgcgtgcaca ccgagacgcg ccagctcccc ctgtattacc
      121 tcgtgcctgc gcggggcgac gggaagctgg gcccgcgcct gagaaagtca gccttcgact
      181 gcgccgcgct gcgcgcacgc aggggcccgg gcggcgcccc gcccgctgac ggctcggagc
      241 cgccgtgtca ggtctcgttc aaggtcggcg acgggagcat gacgattgcg tttcagggcg
      301 agtcggcatc ggagctggct cgcttcgtga ttccggaacg cgatcgtccg gtcatcgaca
      361 agacgaacct cccggggacc ttcgacggcg aattgacgtt cgctccggag ccgctgcctg
      421 ggtttcctcg cttaccgggc agcgagaacg gcgtgtcggt ctatacggcg ctccaggaac
      481 agctgggact gaggctcgag ccggaccacg gaccggtcga gatcatcgtc atcgacggcg
      541 ccgaaaggcc taccgagaac taaggtcttc ctgcgcggaa ctgcaatttc gcgcacacca
      601 tgtaatagtc ggcctgctcg tgcgtcatgg cttggtgagg cggatgcagt gcccacagcc
      661 agcgcgctaa tcaacgagct gccgatcccc atgacttcca cgaccccgct ggcggggttt
      721 gcccggctgt tcgagcgcca ctacgagatg gtcttccgcg cggccctccg cgtcaccggg
      781 aatccggccg acgccgagga cgttttgcag accgtcttcc tgcgcgtcct ggcacagggt
      841 ggagagatcg aggacgtggc gctgccggcc gcctatttcc ggcgtgccgc cgtcaacgcg
      901 gccgtggacg tcctgcgccg ccgggagctt catgcggagt cgtcctacga cgatgaggcg
      961 ccgcaggtcg ccgtccagcc ccctttgctg ctgaaggaac ggctccggcg cgcgatcgcc
     1021 gtcctggaca acgaggacgc gagcctgttc ctgctgcgtc acgtcgaggg gctgtcgatc
     1081 gaagagctgg ccgggatctt caagatcgag aagaacaacg tagccgtcag gctccaccgg
     1141 atcaagcatc ggctgcagac cgagatgcag cgctaacgcg cggtcaagaa ggaggtcggc
     1201 gatgtccgac gatcgtctcg aacgagcact acaggagatg aaagaggaag acgtggactc
     1261 cggcacgatc gaggccgtgc gggcgcgcgt ctgggagaag gtcggcgccg gcagcggcac
     1321 ctgtgcggag ttccgtccgg acttccacgc atatctggcc agtgagctgg gcgccagccg
     1381 acgcctgctg gtggacgatc atctcagccg ctgccccggc tgtcgcgccc ggatcgcgga
     1441 aatgaagggc gagcggacag tcgtcgcgat gccacgcggc ttgccccgcc gtagccttcg
     1501 cgaaggcggg tcctcctccc gcacggtgca gtggggagcc ctggccgcgg cggccgcact
     1561 cctcttcggg gtctatctcg gacgcgacgc tatcgacgcc atgatggctc ctggcggcgc
     1621 gcgggccaca gtcgtgtcc
//
LOCUS       DBNGSH010000602         6194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_909701, whole genome shotgun sequence.
ACCESSION   DBNGSH010000602 DBNGSH010000000
VERSION     DBNGSH010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_909701"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /locus_tag="AC16CY_13940"
     CDS             <1..1818
                     /locus_tag="AC16CY_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3354945.1"
                     /translation="IAESEGQIVLFIDELHTVVGAGAAEGAMDASQMLKPMLARGELH
                     TIGATTLDEYRKHIEKDAALERRFQPVLVGEPTVEDTISILRGLRERYEIHHKVRFKD
                     AALVAAAVLSHRYISDRFLPDKAIDLVDEAASKLRMEIDSMPAELDEVRRRIMQLEIE
                     REALRKETDPASRERLSKLERELADLKEEQTRLQSHWQQEKDAIRGSSALKEQLEEAR
                     RQKDSAERSGDYARASELQYGRLPELERQIAQEEGKLSSLQQAQRMLKEEVDEEDIAE
                     VVAKWTNIPVSRLMEGEIQKLIHMEERLHERVVGQDPAIAAVANAIRRARAGLQDPNR
                     PLGSFIFLGPTGVGKTELARALAEFLFDDEHAMVRIDMSEYQEKHTVSRMIGAPPGYV
                     GYEESGQLTEAVRRRPYAVVLFDEIEKAHAEVLNVLLQLLDDGRLTDGKGRTVDFKNV
                     VVIMTSNLGSQYIAEAQHASGRDELPEGVRRQVTEALRQHFRPEFINRVDEIIFFHSL
                     GLEHLKRIVDIQIRGLLKRLEDRKIHVELSEAARGLLVTEGFDPMFGARPLKRTIQRR
                     VLDPLAMHVLEGRFREGDRIVVDTDEAGLRFDKQQPVSA"
     gene            1927..2223
                     /locus_tag="AC16CY_13945"
     CDS             1927..2223
                     /locus_tag="AC16CY_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354946.1"
                     /translation="MPARAQKASKDPSPFLDVEFKSLPAKAYRRQASPASGEVVDRVR
                     GEFVEMRGFSPTVDQAARLFHINREECDRILAGLVDEGFLKRTPDGRYRLPSQQ"
     gene            2432..2752
                     /locus_tag="AC16CY_13950"
     CDS             2432..2752
                     /locus_tag="AC16CY_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3354947.1"
                     /translation="MSRKLYVGNLPYETNEQDLQELFASAGAVETVTVMRDRDTGRAR
                     GFAFVEMASDDDAQKAITQLNDKQFGGRRLTVNEAKPQVPRSGGGGGGYGNRGPSRRS
                     EPRW"
     gene            2811..3062
                     /locus_tag="AC16CY_13955"
     CDS             2811..3062
                     /locus_tag="AC16CY_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354948.1"
                     /translation="MSVWKQEADVRGYAIGDRVSQPQYGTGTIRLVDQYHTVIDFDEH
                     GLRRFSTAMVKLERSATVEPGKVAKRTKKKAAPKAAKES"
     gene            complement(3066..4730)
                     /gene="ilvD"
                     /locus_tag="AC16CY_13960"
     CDS             complement(3066..4730)
                     /gene="ilvD"
                     /locus_tag="AC16CY_13960"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3354949.1"
                     /translation="MNKYSSRVTQPRSQGASQAMLYGIGLTDADLQKPQVGICSMWYE
                     GNTCNMHLDRLAASVKEGVSAAGLVGLRFNTIGVSDGLSMGTDGMSYSLPSRDLIADS
                     IETVVGAQWYDALITVPGCDKNMPGSVIAMARLNRPALMVYGGTIRAGYAQGKPRDII
                     SAFQSYGEYLAGTITDEQRLDIVRHACPGAGACGGMYTANTMAVAIEALGLSLPYSSS
                     LPAEDGGKADECRRAGAAIGVLLKRDLKPRDILTRAAFENAMTMVMAVGGSTNAVLHL
                     LAIAKAAGVPLALADFQRVSDRVPLLADLKPSGRYVQEDLHAVGGTPAVMRYLLERGL
                     LHGECMTVTGRTLAENLEGLPGLKPGQEIIHTIDDPIHARGHIRILHGNLAPEGAVAK
                     ITGKEGERFAGAAKVFDSEEAMLAGLEKGQIGKGDVVVIRYEGPKGGPGMPEMLTPTS
                     AIMGAGLGSSVALLTDGRFSGGSHGFIIGHVTPEAQEGGPIALVQDGDRITIDAEACS
                     LDVDLTAAQLAARRKAWTAPPVKAAGGVLGKYIRLVRPASEGCVTD"
     gene            complement(4743..5762)
                     /gene="ilvC"
                     /locus_tag="AC16CY_13965"
     CDS             complement(4743..5762)
                     /gene="ilvC"
                     /locus_tag="AC16CY_13965"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR3354950.1"
                     /translation="MATMFYDKDADIELIRQRKVAVIGYGSQGHAHALNLRDSSVDVV
                     VGLQAGSKSVEKAKAAGLKVLSVAEASKWADVIMVLIPDQHQAKVYKAEIAPHLAAGK
                     MLMFAHGFAIRFNWIVPPADVDVTMVAPKAPGHRVREVFTEGAGTPGLLAIHQDVTGK
                     GRATALSYARGIGCTRAGVIETTFAEETETDLFGEQAVLCGGTSALVKAGFETLIEAG
                     YQPEIAYFECLHELKLIVDLMYRGGLQYMRYSISDTAEYGDYTAGARIVTAETKATMR
                     QLLKEIQSGEFARKWIAENENGCPNFKAMREQDRHHPVEVVGARLRDMMPFLDPVKLP
                     QPASV"
     gene            complement(5817..>6194)
                     /gene="ilvN"
                     /locus_tag="AC16CY_13970"
     CDS             complement(5817..>6194)
                     /gene="ilvN"
                     /locus_tag="AC16CY_13970"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR3354951.1"
                     /translation="GISRMTVVIDNDEVNAERVTAYLYKLVNVLQVEDLRDVPAVARD
                     LALVKVAVAGHDRAHLLRVVDETRAHIVDSGEQTMTLEITGEPPHVDAVISRLEPLGI
                     VEMVRTGRVAMRRGDTASAAAQY"
BASE COUNT         1067 a         2032 c         2032 g         1063 t
ORIGIN      
        1 atcgcggagt cggagggcca gatcgtcctg ttcatcgacg agctgcacac cgtcgtcggc
       61 gcgggcgcgg cggaaggcgc gatggacgcg tcgcagatgc tcaagccgat gctcgcgcgc
      121 ggcgagctgc ataccatcgg cgcgaccact ctcgacgaat accgcaagca catcgagaaa
      181 gacgccgcgc tcgagaggcg ctttcagccg gtgctcgtcg gagagccgac cgtcgaggac
      241 acgatctcca tcctgcgcgg cctcagagag cgctacgaga tccaccacaa ggtgcggttc
      301 aaggacgcgg cgctcgtcgc cgccgcggtt ctctcgcacc gctatatctc ggaccgcttc
      361 ctccccgaca aggcgatcga cctcgttgac gaagccgcgt ccaagctgcg catggagatc
      421 gactcgatgc ctgccgagct cgacgaggtg cggcggcgca tcatgcagct cgagatcgag
      481 cgcgaggccc ttcgaaagga gaccgatccg gcatcacgcg agcgcctgtc gaagctcgag
      541 cgcgagctgg ccgacctgaa ggaagagcag acgcggcttc agtcgcactg gcagcaggag
      601 aaggacgcga tccgaggcag cagcgcgctc aaggagcagc tcgaggaggc gcgccggcag
      661 aaggactcgg ccgagcggtc cggagactac gcccgggcgt cggagctgca gtacggccgg
      721 ctgccggagc tcgagcggca gatcgcgcaa gaagagggca agctgagcag ccttcaacaa
      781 gcgcagcgga tgctcaagga ggaagtcgac gaggaagaca tcgcggaggt cgtcgccaaa
      841 tggacgaaca tcccggtcag ccggctcatg gagggcgaga tccagaagct cattcacatg
      901 gaggagcggc tacacgagcg cgtggtgggc caggaccccg ccatcgccgc cgtcgccaac
      961 gcgatccggc gcgcgcgcgc gggactccag gacccgaatc gccccctcgg cagcttcatc
     1021 ttcctcggac cgaccggcgt cggcaagacc gagctcgccc gcgcgctggc cgagttcctg
     1081 ttcgacgacg agcacgcgat ggtccgaatc gacatgtcgg agtaccagga aaagcacacc
     1141 gtgtcgcgca tgatcggcgc gccgcccggc tacgtcgggt acgaagagtc gggccagctc
     1201 accgaggccg tccgcaggcg cccgtacgcc gtcgtgctgt tcgacgagat cgagaaggcg
     1261 catgccgagg tgctgaacgt cctgctccag ttgctcgatg acggccggct caccgacggg
     1321 aagggacgta cggttgactt caagaacgtc gtcgtgatca tgacgtcgaa tctcggcagc
     1381 cagtacattg ccgaggcgca gcatgcttcg gggcgcgacg agctgcccga aggcgtacga
     1441 aggcaggtca ccgaggcgct gcggcagcac ttcaggccgg agttcatcaa ccgcgtggac
     1501 gagattatct tcttccactc gctgggcctc gagcacctga agcgcatcgt ggacattcag
     1561 attcgtggat tgctcaagcg gctcgaggac cggaagattc acgtcgagct cagcgaggcc
     1621 gcgcgcggcc tgctggtcac cgagggcttc gatccaatgt tcggcgcgcg gccgctgaag
     1681 cgcacgatcc agcggcgcgt gctggatcct ctcgcgatgc acgtgctcga gggccgcttc
     1741 cgcgaaggcg acaggattgt cgtggatacg gacgaggccg gactgcgctt cgacaagcaa
     1801 cagccagtct ctgcctgatt cgggtccgct agacaacgtc gagacgcgat gggttctgtc
     1861 cttgacccat cgcgctcgca tgacatatcg tctaagaaca caccatctct cgccggggag
     1921 gtttttatgc ccgcgcgtgc ccagaaagcc tcgaaggatc cttccccctt cctcgacgtg
     1981 gagttcaaat cgcttcctgc caaggcgtat cgccggcagg catcgcctgc gtccggtgaa
     2041 gtcgtcgacc gggtgcgggg agagttcgtg gaaatgcgcg gattctcacc gaccgtggac
     2101 caggcggcgc ggttgtttca catcaaccgc gaggagtgcg atcgcatcct ggccggcctt
     2161 gtcgacgagg gattcctgaa acggacgccg gacgggcggt atcggctgcc atctcagcag
     2221 taggccgttt cgtctagttg tgccagtttg ggtgtataat cccgcggtct ggaagcttag
     2281 cccaccgctt agtcgcgttc agcgtcgatc cgaccgtcct ctgatcttcc tgcatgcagt
     2341 tgagccgagc gacagtcgct caggctgggt tttcgtcagc gctccacgcg tcgtggagta
     2401 gtcgcatccg aagggatgca ggagtcagtc gatgagccgt aagctatacg ttggaaacct
     2461 gccctacgaa accaacgaac aggacctcca ggagctgttc gcctcggcgg gggccgtcga
     2521 aacggtgacc gtcatgcgcg atcgggacac gggccgcgcc cgtgggttcg ccttcgtcga
     2581 gatggcgagc gatgatgacg cgcagaaagc catcacccaa ctcaacgata agcagttcgg
     2641 cgggcgccgg ctgacggtca acgaggccaa gccgcaggtg ccacgttccg gcggcggtgg
     2701 cggtggatac gggaatcgcg ggccgtcgcg tcgcagcgag ccccgctggt agacggcgca
     2761 tcagcgtatt caggtcggtg tgatccgcgt ccatcaggac gcggtgctcg atgagcgtgt
     2821 ggaagcagga ggcggacgtg cgaggttatg ccattggcga ccgggtctct cagccgcaat
     2881 acggcacggg tacgattcgg ctcgtagacc agtaccacac cgtcatcgat ttcgacgagc
     2941 acgggcttcg ccgcttctcc acggccatgg tgaagctgga gcgatctgcg acagtcgagc
     3001 ctggcaaggt ggcgaagcgg acgaagaaga aggctgctcc gaaggccgca aaggaatcct
     3061 gacggtcagt ccgtcacaca cccttccgac gctggcctga ccagccggat gtacttcccg
     3121 aggacgccgc ccgccgcctt gacgggtggg gcggtccacg ccttgcggcg cgcggcgagt
     3181 tgcgccgcgg tcagatcgac gtcgagcgaa caggcctccg cgtcgatcgt gatgcggtcg
     3241 ccgtcctgga cgagcgcgat cggcccgcct tcctgcgcct ccggcgtaac gtggccgatg
     3301 atgaagccgt gcgatccgcc ggagaatcgc ccgtcggtga ggagggccac gctggatccg
     3361 agcccggcac ccatgatcgc ggaggtcggc gtcagcatct cgggcatgcc cggcccgccc
     3421 ttgggacctt cgtagcgaat cacaacgacg tcgcccttcc cgatctgccc tttctcgaga
     3481 cccgcaagca tcgcctcttc ggaatcgaag accttggccg cgccggcaaa gcgctcgcct
     3541 tccttgccgg tgatcttggc cacggcgccc tctggcgcga gatttccgtg caggattcga
     3601 atgtgccccc tcgcgtggat cgggtcgtcg atcgtgtgaa tgatctcctg tcccggcttg
     3661 agcccgggga ggccctcgag gttttctgcc agcgtgcggc ccgtgacggt catgcattcg
     3721 ccgtgcagca gaccacgctc gagcaggtag cgcatcacgg ctggcgtgcc gccaacggcg
     3781 tgcagatctt cctgcacgta ccggccgctc ggcttcagat ccgcgagcag cggcacgcgg
     3841 tcgctcaccc gctggaaatc cgccagcgcc aggggcacgc cggcggcctt tgcgatcgca
     3901 agcaggtgga gcacggcgtt ggtcgagccg ccgacggcca tgaccatcgt catggcgttc
     3961 tcgaacgccg cacgcgtcag gatgtcgcgg ggcttcagat cgcgcttcag gaggacgccg
     4021 atcgcggcgc ccgcgcggcg gcactcgtcg gcctttcccc cgtcctcggc cggcagcgat
     4081 gagctgtacg gaagcgaaag gcccagcgcc tcgatggcca ccgccatcgt gttggccgtg
     4141 tacattccgc cgcacgcgcc ggcgccggga cacgcgtgcc gcacgatatc cagccgctgc
     4201 tcgtcggtga tggtgccggc aaggtattca ccgtaggact gaaacgccga gatgatgtcg
     4261 cgcggcttcc cctgcgcgta gcccgcccgg atcgttccgc cgtataccat cagcgccggc
     4321 cggttgagcc gcgccatcgc gatgacgctt cccggcatgt tcttgtcgca gccaggaacc
     4381 gtgatgagag cgtcgtacca ttgagcgccc accacggttt cgatcgagtc ggcgatcagg
     4441 tctctggacg gcaacgagta gctcatgccg tcggtgccca tcgacagccc gtcgctgacg
     4501 ccgatcgtgt tgaagcgcag tccgaccagt cctgccgcgg acacaccttc cttgacgctc
     4561 gccgcaaggc ggtcgaggtg catgttgcac gtgttgccct cgtaccacat gctgcagatg
     4621 ccgacctgcg gcttctgcag gtcggcgtcg gtcaggccga tgccgtacag catcgcctgg
     4681 gacgcaccct ggcttcgggg ctgtgtgacg cgcgacgaat acttgttcac gtgcctggtg
     4741 atctacacgc tcgccggctg tgggagcttc acagggtcga ggaacggcat catgtcccgg
     4801 agcctggcgc ccaccacctc gacggggtga tgacggtcct gctcgcgcat cgccttgaag
     4861 ttcgggcagc cgttctcgtt ctccgcgatc cacttcctcg cgaactctcc cgactggatc
     4921 tccttgagca gctgccgcat tgtcgccttc gtctcggccg tgacgatccg cgcgccggcc
     4981 gtgtagtcgc cgtactctgc ggtgtcgctg atcgagtagc gcatgtactg aagtccaccg
     5041 cggtacatca ggtccacgat gagcttcagc tcgtgaaggc actcgaaata cgcgatctcc
     5101 ggctgatagc cggcctcgat cagcgtctcg aacccggcct tgacgagcgc actcgtgccg
     5161 ccgcacagaa cggcctgctc gccgaacagg tcggtctcgg tttcctcggc gaaggtggtc
     5221 tcgatgacgc cggcgcgggt gcagccgatg ccacgggcat aggacagcgc agtggcccgc
     5281 cccttcccgg tgacgtcctg atggatcgcg agaagccccg gagtgccggc gccttcggtg
     5341 aacacctctc gcacccgatg tccgggcgcc ttcggcgcga ccatcgtcac gtccacgtcg
     5401 gcgggcggaa cgatccagtt gaaacggatc gcaaagccgt gcgcgaacat cagcatcttg
     5461 cccgcggcga ggtggggcgc gatctctgct ttgtacacct tcgcctgatg ctggtccggg
     5521 atgagcacca tgatgacgtc cgcccacttc gaggcctccg ccaccgacag caccttgagt
     5581 ccggctgcct tggccttctc caccgacttg ctgccggcct gcaggccaac gacgacgtcg
     5641 acgctgctgt cccgaaggtt cagggcgtgc gcatggccct gcgagccgta gccgatgacg
     5701 gccacttttc gctggcgaat gagctcaatg tcggcgtctt tgtcgtagaa catcgtggcc
     5761 atggtgtctc ctcactcaat gcgcgggtgc aaggctcggg gtgtcgggag cctcgctcag
     5821 tattgtgcgg ccgccgacgc cgtgtcgccg cggcgcatcg ccacacgtcc ggtccggacc
     5881 atctcgacga tccccaacgg ctccaggcgc gagatgacgg cgtctacgtg cgggggctcg
     5941 cccgtgatct cgagcgtcat cgtctgctca cccgaatcga cgatgtgggc gcgcgtctcg
     6001 tcaaccacgc ggagcaggtg cgcgcggtcg tggcccgcga ccgccacttt cacgagcgcg
     6061 aggtcgcgcg ccaccgcggg aacatcgcgg agatcctcga cctggagcac gttcacgagc
     6121 ttgtagagat acgccgtgac gcgctcggcg ttgacttcgt cgttgtcgat gacgaccgtc
     6181 atgcgcgaaa tacc
//
LOCUS       DBNGSH010000603         4154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1248214, whole genome shotgun sequence.
ACCESSION   DBNGSH010000603 DBNGSH010000000
VERSION     DBNGSH010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1248214"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="AC16CY_13975"
     CDS             <1..861
                     /locus_tag="AC16CY_13975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3354952.1"
                     /translation="MVKAATSVACAAALVASAAAVSSAQGRGGRAGGAGGAGTVERIT
                     VHGKALEGSAEAGSPDRQVTVYLPPGYAADQNRRYPVVYLLHGDGGRDDTFTAGPGGL
                     QESADRLAAAQGFSVAIVVTPDGSSSGKGSLSAAPEWERFVAEDLVAHVDGRYRTLAS
                     RMSRGLAGHAAGGTSALRIGMKRPDVYLSLYVMSAASPPAEMLDQYASNLKKYYAIAI
                     DVGTADPARPSNRQFHDAMTRLRVPHGYEEYDSDRASRIRERIERNVLPFFSMNLVAP
                     ANPTSPLPPR"
     gene            complement(863..2485)
                     /locus_tag="AC16CY_13980"
     CDS             complement(863..2485)
                     /locus_tag="AC16CY_13980"
                     /inference="COORDINATES: protein motif:HMM:NF021379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HIR3354953.1"
                     /translation="MIQSTRTATALIAVGVGVATILFAAPFAGWPVVRLPAVILPALF
                     IVAIIFRPWRWDDRRLRAIDAWTPPARVVWSAALVTGLALFWFVLTRFQSGEINGVDF
                     TVYFDRPLFQTLQGRLLFVESADDVLRAQKTLLIIHAYWALVPLALLYGIHATPLWLL
                     ALSVAAVVAGSVHVFRILQRLGAGSLLAGASALAFVLNDNTARTLNYGFHPEVLYAWF
                     IPWLLDAGLAGRRMSYLAAAAACVLVKEDACLLLLASAVALGLHRYHELTWADRLFFL
                     AAPTLAALVNLAMYYGAIVPALTGGSVLIYGNYWVGYGATPLQAALGMARQPVRVLSS
                     ALTSGFFTRVILPHLFLPLVGWRWTLGIAPIVALYGASSNDQLRGYGIYYAVVLIPFL
                     VIGASIGALAIVDRLPIAAARRRPLAALIVLAGALLVGSTSAGYSLRPWRAEVGSVRD
                     LVEQFDGEPVVLVQSGLYPHTGYDERIQLLTPDAVTDAGNAGAVVVLAPAIGAYPFSR
                     ADIDALAALPALRPPADGLLAVRLTALPARYQ"
     gene            complement(2577..3470)
                     /locus_tag="AC16CY_13985"
     CDS             complement(2577..3470)
                     /locus_tag="AC16CY_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3354954.1"
                     /translation="MSFKPLAPVCLFICAAVAGLGAQQQLSFLATVTDPATGVAVEAL
                     NPADVRATEDGATLKVLKVESVKRTVKVQLLIDTGLGIPAESVTQLRAGVRGLVEALP
                     PDMEITLVTTSPQPRFLVRNTKNREELLKGVDRLAPDRGAGRFVESLSEAAERASKDP
                     EGTFNIVIAAGTTAGDTVVADGDISRLMKFLRSRPMMVHVLIFSGPLNTNLSGGIAQT
                     EVGTAVSQGTRGRYEVISNMSRYVTLLTELGAEVSKQAAGRANQWRVFVQRPDGKSGK
                     PGRVSLGVDGKAVPDVILEGF"
     gene            3666..>4154
                     /locus_tag="AC16CY_13990"
     CDS             3666..>4154
                     /locus_tag="AC16CY_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354955.1"
                     /translation="MRGTRFLALFTSASLAALTAWTGVGPVSTGVFAQAPAPAGQGGR
                     GGAPPGAPGGGRGRGRAPVIQGPPAGVQPLAIDLFSSKNFYKDRANWLDKRYYRCNNP
                     RQLYAMWDSQRIGATPPESASWGNCDDDWPRERIVSPYPYKTAKEHYDALMAQAKAKG
                     GPT"
BASE COUNT          737 a         1394 c         1425 g          598 t
ORIGIN      
        1 atggtgaagg cggcgacgtc ggtggcgtgc gcggccgctc tggtcgcgag tgctgccgcg
       61 gtgtcgagcg ctcaaggtcg cggaggccgc gccggaggcg caggtggagc tggaacggtc
      121 gagcgcatca ccgtgcacgg caaggcgctc gagggaagcg cggaggcagg ctctcccgat
      181 cgccaggtca ccgtgtacct ccctccgggc tatgccgccg accagaaccg gcggtacccg
      241 gtcgtctatc tcctgcacgg tgacggcgga cgcgacgaca ccttcacggc cggcccgggc
      301 ggtcttcaag agagcgccga ccgcctcgct gccgcccagg gtttcagcgt ggctatcgtc
      361 gtcacgccgg acgggtcctc gtcgggcaag ggcagcctgt ctgccgcacc agagtgggag
      421 cggttcgtgg cggaggatct cgttgcacac gtcgatgggc ggtaccgcac gctcgccagc
      481 cgcatgagcc gtggcctggc gggtcatgcg gcggggggaa caagcgcgct ccggatcggg
      541 atgaagaggc ccgacgtcta cctgagtctt tacgtcatga gcgccgccag cccgccggcc
      601 gaaatgctcg accagtacgc gtcgaacctg aagaagtact acgccatcgc catcgacgtc
      661 ggtacggccg acccggcccg cccgtccaac cggcagtttc acgacgcgat gacgcgcctg
      721 cgcgtccccc acggctacga ggaatacgat agcgatcgcg ccagccggat ccgcgagcgg
      781 atcgagcgga acgtgctgcc gttcttctcg atgaacctcg tggcgccggc gaacccgacc
      841 tcgccattgc ccccgcgctg acctattggt accgggcggg cagcgccgtc agccggacgg
      901 ccagcagacc gtctgccgga gggcgcagcg ccggcagcgc cgcgagcgca tcgatgtcgg
      961 cacgcgagaa ggggtacgcg ccgatggcgg gcgcaagcac cacgacggcg ccggcattgc
     1021 cggcgtccgt cacagcgtcc ggcgtgagca actggatccg ctcgtcgtac cccgtgtgcg
     1081 gatacaggcc gctctgcacg agtaccactg gctcgccgtc gaattgctca acgagatccc
     1141 ggaccgagcc gacctccgcc cgccagggcc gcaggctgta cccggcgctc gtgctcccca
     1201 cgaggagcgc gcccgccaga acgatcaggg cggccagggg ccggcgacgg gccgcggcga
     1261 tggggagacg atccacgatg gccagggcgc cgatcgacgc gccgatgacg aggaacggga
     1321 tcagcacgac ggcgtaatag atgccgtagc cgcgtaattg atcgttcgac gaggcgccgt
     1381 acagcgccac gatcggcgcg atgccgagcg tccatcgcca gccgaccaga ggcaggaaca
     1441 ggtgcggcag gatcacacgg gtgaagaacc ccgacgtgag cgcgctcgag agtacccgga
     1501 cgggctggcg cgccatgccc agcgccgcct gaagcggggt cgcaccgtag ccgacccagt
     1561 agtttccgta gatgagcacg ctgcccccgg tgagcgcggg aacgatcgcg ccgtaataca
     1621 tcgccagatt caccagcgcc gccagcgtcg gcgcggccag gaaaaacagg cgatcggccc
     1681 acgtcagctc gtgatagcgg tgcagcccca gcgcgacggc cgacgcgagc agcagcaggc
     1741 aggcgtcctc cttcacgagg acgcacgcgg ccgcggcggc gaggtacgac atccgccgcc
     1801 ccgcgaggcc cgcgtccagc agccacggaa tgaaccacgc gtagagcact tcggggtgga
     1861 agccgtagtt gagcgtccgc gcggtgttgt cgttgagcac gaacgccagc gccgacgcgc
     1921 ctgcgagaag cgaccccgcg ccgagccgct gcaggatcct gaagacgtgc acggagccgg
     1981 cgaccacggc cgcgaccgac aacgcgagaa gccatagcgg cgtcgcgtgg atgccgtaca
     2041 ggagcgcgag cggcacgagc gcccagtatg cgtggatgat caacagcgtc ttctgcgccc
     2101 tcagtacatc gtcagccgac tccacaaaga gcagccgccc ctgcagcgtc tggaacagcg
     2161 ggcggtcgaa gtacaccgtg aaatccacac cgttgatctc gcccgattga aacctggtca
     2221 ggacgaacca gaacagcgcg aggcccgtga cgagggcggc cgaccacacg acgcgcgccg
     2281 gcggcgtcca cgcgtcgatc gcgcgcagcc ggcggtcgtc ccaccgccac ggccggaaga
     2341 tgatggcgac gatgaagagc gccgggagga tgacggcggg caggcggacg acaggccatc
     2401 ccgcgaaggg ggcggcgaac agtattgtgg caacgccgac accgacggcg atgagcgccg
     2461 tcgccgtcct agtggactgt atcaactgaa cctgtagcga gtcggacagc cctacgacgc
     2521 cgatttcgcg ggccaaccaa cgggcagccc gccttacgtt tcgctaatac gctccactag
     2581 aacccttcga ggatgacgtc cggcacggct ttcccgtcca ctccgaggct gactcggccg
     2641 ggcttccccg atttgccgtc cggacgctgc acgaagactc gccactggtt ggcccgtccc
     2701 gcagcctgct tggagacctc ggcgcccagt tccgtcagga gcgtgacgta tcgcgacatg
     2761 ttgctgatga cctcgtaacg cccgcgcgtc ccctgggaga cggccgttcc cacctcggtc
     2821 tgggcgatgc ctccgctcag gttcgtattc agggggcccg agaagatcag gacgtggacc
     2881 atcatcggcc ggctccgcag gaacttcatc aggcgcgaga tgtcgccgtc ggcgacgacg
     2941 gtgtcgccag ccgtcgttcc ggctgcaatc acgatgttga aggtgccctc cggatccttc
     3001 gacgcgcgct cggccgcttc gctgaggctc tccacgaagc gcccggcgcc gcggtcggga
     3061 gcgagtcgat cgacaccctt gagcagttct tcacggttct tggtattcct gacgaggaac
     3121 cgcggctgcg gggaggtggt aacgagcgta atctccatgt cgggcggcag cgcttcgacg
     3181 agcccccgaa cgccggcacg cagctgggtg acgctctcgg cgggaatgcc gagtccggtg
     3241 tcgatgagga gctgcacctt cacggtgcgc ttgacggatt caaccttgag gaccttgagc
     3301 gtggcgccgt cttcggtggc gcggacatcg gcgggattca gcgcctccac cgcaactccc
     3361 gttgccggat cggtgacagt ggccaggaac gacaactgct gctgagctcc cagccccgcg
     3421 accgcggcgc agatgaacag acagaccggg gcaagcggct tgaacgacat acaacgctcc
     3481 tcgatgttag gcaggctggg tcgcatctta gaccacgggg cacgatggct ttgagacgat
     3541 caatatctat tccgagtttc ccggttctgg gttcccggtt cctattcgca gaccgaaacg
     3601 ggtggctgtt tcaacatgta tgatccgagg tgctgcgata cgagcacccg cgaggagggg
     3661 ctcacatgag aggaacgcgg ttcctggcac tgtttacgtc cgcgtccctg gccgcgttga
     3721 ctgcgtggac cggggtcggg ccggtctcga ctggcgtttt cgcgcaggct ccggccccgg
     3781 cggggcaggg gggccgcggc ggggcacctc ccggtgcccc gggaggcgga cgcgggcgcg
     3841 ggcgggcccc cgtgatccag ggtcctcccg caggcgtcca gcccctggcc atcgacctgt
     3901 tctcgtcgaa gaacttctac aaggacaggg cgaactggct ggacaagcgc tactaccgct
     3961 gcaacaaccc gcgccagctg tacgcaatgt gggattcaca gcggatcggg gccacgcctc
     4021 ccgagtcggc gtcgtggggc aattgcgacg acgactggcc gcgcgagcgc atcgtcagtc
     4081 cctacccgta taagacagcg aaggaacatt acgacgcgct gatggcccag gcgaaggcaa
     4141 agggcggtcc gacg
//
LOCUS       DBNGSH010000604         1920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_400166, whole genome shotgun sequence.
ACCESSION   DBNGSH010000604 DBNGSH010000000
VERSION     DBNGSH010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_400166"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..797
                     /locus_tag="AC16CY_13995"
     CDS             60..797
                     /locus_tag="AC16CY_13995"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3354956.1"
                     /translation="MLEVHDLHMRFGPVIALAGVSFAAPDGQITGLLGENGAGKTTTM
                     NILTGLFQPHRGDIRIDGEPAGATVHRRRSVGALLDQKGLYPRLTARENVAYFGALRG
                     LEGRELTRAVDRALALVGLEPVASRRTEGFSQGERMKVALARAIVHSPRNLLLDEPTN
                     GLDIPTARGFKAALRDMRERGVCIVFSSHILDDIRSLCDSVVIISKGRLVARGTPPAL
                     CAQAGCATLEDAFIRLTGREELACLPG"
     gene            782..1558
                     /locus_tag="AC16CY_14000"
     CDS             782..1558
                     /locus_tag="AC16CY_14000"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3354957.1"
                     /translation="MSARLTIAAKEIRDHLRDRRSLASAAMFSLMGPAVILLVSQSAA
                     ASKGGPAVLLGMMSVFTLVSSFTGGMYLAMDSTAGERERGSLVPLLLNPVSRLDLVIG
                     KWLAASAFALAGLALNLAGFTLAFEWAGVVPPDGFSRVFLLWTVCGLVPLALLGAALD
                     LLAAATCRTTKEAHVRLTIVTFVPMIVGMALIFFPGWVGRWWYALPVVGQQALIGAGL
                     RGDVVSVVQSSTLGLVTIAFAALALHAAARAFDRDEILAA"
     gene            1560..>1920
                     /locus_tag="AC16CY_14005"
     CDS             1560..>1920
                     /locus_tag="AC16CY_14005"
                     /inference="COORDINATES: protein motif:HMM:NF016430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3354958.1"
                     /translation="MSADATTIWSELSAVELEHRLDSLLALHGASLARLAGSYVREAG
                     DRDDLLQEIAVAVWQALPRFRGECSERTFLFRIAHNRAIAFISRRHLPLRETDDDFDV
                     EDGRPNPEEALATGQQGR"
BASE COUNT          259 a          648 c          674 g          339 t
ORIGIN      
        1 gcggtcctgt aaggaaactt ccgcgaccga gactacctgt ccaggaggcg gcaggagcca
       61 tgctggaagt tcacgacctg cacatgcgtt tcggcccggt catcgcgctg gcgggtgtgt
      121 cgtttgccgc gcccgacggc cagatcacgg ggctgctcgg cgaaaacggc gcgggcaaga
      181 cgacgacgat gaacatcctc accgggttgt tccaacccca tcgaggagac atccgcatag
      241 acggcgagcc tgccggcgcg acggtgcatc gccggcgatc ggtcggcgcg ctgctcgacc
      301 agaagggtct gtatccccgc ttgaccgcgc gcgagaatgt cgcgtacttc ggcgcgttgc
      361 gtgggctcga agggcgcgag ctgacgcgcg ccgtcgatcg cgcactggcg ctcgtcggcc
      421 tcgagccggt cgcgagccgc cggaccgaag gcttctcgca gggggagcgg atgaaggtcg
      481 cgctcgcccg cgcgatcgtg cattcgccgc ggaacctgct gctcgacgag cccaccaacg
      541 gcctcgacat cccgaccgcg cgcggcttca aggccgccct gcgcgacatg cgcgagcgcg
      601 gagtgtgcat cgtgttctcg agccacatcc tcgacgacat ccggtcgctc tgcgacagcg
      661 tcgtgatcat ctcgaaggga cgtctcgtgg cccgcggcac gccgccggcc ttgtgcgcgc
      721 aggccggctg cgccacgctg gaagacgcgt tcatccggct caccggacgg gaggagctcg
      781 catgtctgcc aggctgacga tcgcggcaaa ggagatccgg gatcatcttc gcgaccgccg
      841 atcgctcgca tcggcggcga tgttctcgct gatgggcccg gccgtgatcc tgctggtgtc
      901 gcagtcggcc gccgcaagca aaggcggtcc cgccgtgctg ctcggcatga tgtccgtctt
      961 cacgctcgtg tcgtcgttca cgggcggcat gtatctcgcc atggactcga cggccgggga
     1021 acgcgagcgc ggctcactcg tgccgcttct gctcaatccg gtgtcgcggc tggacctcgt
     1081 gatcggcaag tggctggccg catcggcctt cgcgctggcg ggactcgccc tcaaccttgc
     1141 cgggtttact ctcgcgttcg agtgggccgg cgtcgtgccg ccggacggat tctcccgggt
     1201 gttcctgctg tggaccgtct gcgggctggt gcctctggcg ctgctcggcg cggcactcga
     1261 tctgctcgcg gccgcgacgt gccggaccac gaaagaggcg cacgtcaggc ttacgatcgt
     1321 gacgttcgtt ccgatgatcg tcggcatggc gctcatcttc tttccgggct gggtgggccg
     1381 ctggtggtac gcgctgccgg ttgtcggcca gcaggcgctg atcggggcgg gcttgcgtgg
     1441 cgacgtcgtg tcggtggttc agtcctctac gctgggcctc gtgacgatcg ctttcgccgc
     1501 gctcgcgctg catgccgcgg cgcgcgcgtt cgatcgggac gagattctcg cagcttgacg
     1561 tgagcgctga cgccaccacg atctggagcg agctgtccgc ggtcgaactc gaacaccggc
     1621 tcgactccct cctggctctg cacggcgcgt cgctcgcgcg cctggcgggg agctatgtgc
     1681 gagaggccgg cgaccgcgac gacctgctgc aggagatcgc ggtcgccgtc tggcaggcgc
     1741 tgccacgctt ccgcggcgag tgctcggagc ggacgttcct gtttcgcatc gcccacaacc
     1801 gggcgatcgc gttcatctcc cgccggcacc tgccgctgcg ggagaccgat gatgacttcg
     1861 atgtcgagga cggccgtccc aatcctgagg aggcgctggc gaccgggcag cagggacggc
//
LOCUS       DBNGSH010000605         4848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1248476, whole genome shotgun sequence.
ACCESSION   DBNGSH010000605 DBNGSH010000000
VERSION     DBNGSH010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1248476"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC16CY_14010"
     CDS             <1..335
                     /locus_tag="AC16CY_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354959.1"
                     /translation="AAALRSRPLGSKPLASMAMAVMVTMLVAGLWHGTTFSFVLFGVV
                     HGVYLVVFRTYEHVALSVLGRRGLRQLRSSRAWLVASTIVTFHFTATAYIFFVLDTNR
                     LMLILERF"
     gene            461..2335
                     /locus_tag="AC16CY_14015"
     CDS             461..2335
                     /locus_tag="AC16CY_14015"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HIR3354960.1"
                     /translation="METGITPTVGPAPPEEERAASAWARLLAQASTCTDAVAFQALAR
                     KAARLEKELGGPPGHMPARIALMGGASTDLLVRPLGLALRLVGFAPELHVTPYNVMLQ
                     EMLEPDSGTSRFGPQIAIALMTPHSLPDWPAPGATADEATDLARRTCQQLYDACSVLH
                     ERCNAEIVLNTLPPFPQQPMGNLSAKSPEDANNFIRRVNVMLGDLAPPYVHMNDVAGL
                     VERRGLDRWFDVRLWHEAKQPVAPGLIPELVRNMAAIVGGILGRSRKCLVVDLDNTLW
                     GGVVGDVGVEGLALGEGTGLGEAFKAFQLYLRSLKERGVLLAVSSKNEERHALKPFEE
                     HPETVLRRDDFVAFRANWSPKSDNLRAIARELNLGLDALVFIDDNPAEREEVRQTLPQ
                     VVVPELTDDPADYPMIVDATRCFEVPSVTEEDRRRSGMYRQRGQQHELLGATRNLSEF
                     LASLEMRAVIRPFEPVSFARITQLVNKTNQFNLTTRRLTGAQVEALAADPKSFTRTVR
                     LQDRFGDHGLISVLFGRIEADTLTLDGWLMSCRVLNRGVEQRLLNEVVAGMARAGVRY
                     LVGSFIPTDRNALVRDLYRDLGFVLVSERDGVTTWRLDVHGFEPLQTFVTVRAEEGVE
                     "
     gene            2332..2589
                     /locus_tag="AC16CY_14020"
     CDS             2332..2589
                     /locus_tag="AC16CY_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3354961.1"
                     /translation="MTTGIDVLERVRLVIARVFGDDDVVVTRSTTASDVEGWDSVSNI
                     EVLVALEREFAVRFNTGEMATLANVGQLVELIELRLRRGED"
     gene            2600..3394
                     /locus_tag="AC16CY_14025"
     CDS             2600..3394
                     /locus_tag="AC16CY_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3354962.1"
                     /translation="MEAFYFGPNERRLFGVYQPPVVESPVAAAAVLCYPFLQEYLRSH
                     RAFHRLAGLLTARGWHVLRFDYSRCGDSYDGPPDVDAWRKDVKLACDELRERAQTRRL
                     ALIGLRQGASLAASVSCDMQPISDLVMWDPVIDGPAFRQELLGFRYSVTLPPGARRKL
                     ERLDPGDRGVGLTLGPDVLEAIGRLDRTQQLKASADRILVLDSQGDESAVDFARRLEN
                     LRPNVTLIRVSERGVWVERKRKDPRNISVPTVSLQAIVDWMSRTEG"
     gene            3391..4284
                     /locus_tag="AC16CY_14030"
     CDS             3391..4284
                     /locus_tag="AC16CY_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3354963.1"
                     /translation="MREHAVVFGNESPLVGIVTEPDSRLNGAPVCVLLNAGLGHRVGP
                     NRLNVSLARKLADVGLTSLRFDFSGIGDSPASPSLDDAEERGVNETMQAMDFLAESLG
                     PSRFVPIGLCSGANVAFALARRDKRVVGAVLINAATVPSAHSDDQMKEARTRAEAHHH
                     EARLADWKSWLRVLTARSDLAAVTRSTLRLARRAAGLKEQPLREFDLGVLPELDRRGV
                     ELLVMYTEGDIGLELLASHVGPFENLASLERVRLEILRETDHILTPLWAQAQVEEMML
                     DWMTRRLGVGRAATRAESHAL"
     gene            4281..>4848
                     /locus_tag="AC16CY_14035"
     CDS             4281..>4848
                     /locus_tag="AC16CY_14035"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013166.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase II family protein"
                     /protein_id="HIR3354964.1"
                     /translation="MRSLRSFAEGRWFVVPRPLDDPRLRLFCFPYAGGGAFAFRKWAE
                     QVPRDVEFCGVQLPAREGRFKEPAYTRLTDLVRDLAEVVGPATDVPYAFFGHSMGALV
                     AFALARELRRVNARGPELLMVSGRRAPQRPDPDPPIHALPMAEFLQELRELNGTPEAI
                     FRDEELLQLLVPILRADFEICETFEYQQE"
BASE COUNT          796 a         1498 c         1636 g          918 t
ORIGIN      
        1 atgcggcagc ccttcgttcg aggccgctgg gaagcaagcc actggcgtcg atggcgatgg
       61 ctgtcatggt cacaatgctc gtggctggtc tctggcacgg cacgacgttc agtttcgtcc
      121 tgttcggcgt cgtccacggc gtctaccttg tcgtgtttcg aacctatgaa catgtggcgc
      181 tctcggtgtt gggccgccgc gggctgcgac aattgagatc cagccgcgcg tggctcgtcg
      241 cctcgacgat cgtcacgttt cacttcacgg ccaccgcgta catcttcttc gtgctcgaca
      301 ccaacaggct catgctgatc ctggagcgct tttgatgcgc ctgagactga cagcgaagct
      361 gctgtggacg tgggtcctcg tctttctcct cgtgacgttc gcgagcagcg aagtcgattt
      421 cgtataccgt ggtttctgag cagcgtgtga accgctagtc gtggaaacgg ggattacacc
      481 aaccgtcgga ccggcaccgc ccgaggagga gcgcgcggcg tcagcctggg cgcgtcttct
      541 ggcgcaggcc tcgacgtgta cggacgcggt cgcgtttcaa gcgctggccc gcaaggccgc
      601 gcgattggag aaggagctcg gcgggccacc cggccacatg ccggcgcgga tcgcgctgat
      661 gggcggggcg tcgaccgacc tgctcgttcg gccattgggc ctggcccttc gcctcgtcgg
      721 gtttgctccg gagctgcatg tgacgccgta caacgtcatg ctccaggaga tgctggagcc
      781 ggacagcgga acctcccggt ttggtccgca gatcgcgatt gccctcatga cgccgcacag
      841 tctcccggac tggcccgccc ccggagcgac cgcggacgaa gcgacggacc tggcccggcg
      901 gacctgtcag cagttgtatg acgcgtgcag tgttctgcac gagcggtgta acgccgagat
      961 cgtgctcaac acattgcccc ccttcccgca acaaccgatg gggaatctct cggccaagag
     1021 tccggaggac gccaacaact tcattcgtcg ggtcaacgtc atgctcggag atctcgcacc
     1081 cccgtacgtg cacatgaacg atgtggcggg gctggtcgag cggcggggac tggatcgatg
     1141 gttcgacgtc aggttgtggc acgaggcgaa gcagccggtc gcaccggggc tgatccccga
     1201 actggtccgg aacatggctg cgatcgtcgg cgggattctc ggccgaagcc gcaagtgcct
     1261 cgtggtggac ctggacaaca cactctgggg aggcgtggtg ggcgacgtgg gcgtcgaggg
     1321 gctcgctctc ggtgaaggga caggcttagg cgaggccttc aaggcgtttc agttgtacct
     1381 caggtcgctc aaggaacgcg gcgtgctcct cgccgtcagc agcaagaacg aagagcgtca
     1441 cgcgctgaaa ccgttcgaag agcatccgga aacggtgctc aggcgcgacg acttcgttgc
     1501 gttcagggcc aactggagtc cgaagtccga taacctgcgg gccatcgccc gtgagttgaa
     1561 cctgggcctg gatgcgttgg tcttcatcga cgacaatccg gcagaacggg aagaggttcg
     1621 gcagaccctg cctcaagtgg ttgtgcccga gctcactgac gatcctgccg actacccgat
     1681 gatcgtcgat gccacacgct gcttcgaggt gcccagcgtc acggaagaag atcggcggcg
     1741 ctccgggatg taccggcagc ggggccagca acacgagctc ctgggtgcca cgcgcaatct
     1801 gtcggagttc ctggcatcgc tcgagatgcg ggccgtcatt cgcccgttcg aaccggtctc
     1861 gttcgcccgc atcacacagc tcgtcaacaa gacgaaccag ttcaatctca ccacgaggcg
     1921 gttgactggt gcgcaggtgg aagcgctggc ggctgacccg aagagcttca cgcgtacggt
     1981 ccgactccag gatcgcttcg gcgaccacgg actcatcagc gtcctcttcg gtcgaatcga
     2041 ggccgacacg ctgaccctgg acggctggct catgagctgc cgcgtgctca atcggggagt
     2101 cgagcaacgg ctgctgaacg aggtcgtggc cggaatggct cgcgccggcg tccgctacct
     2161 ggtgggcagc ttcatcccca ccgatcgcaa cgccctggtg agggatttgt accgcgatct
     2221 cgggttcgtg ctcgtgagcg agcgtgacgg tgtgaccacg tggcgtctgg acgtgcacgg
     2281 cttcgagccg ctgcagacct tcgtgacggt ccgggcggaa gaaggcgtgg aatgacgacg
     2341 ggcatcgacg tgctggaacg cgtacgattg gtgatcgccc gcgtgttcgg cgacgacgac
     2401 gtcgtggtga cgcgctcgac gacggcgtcc gatgtggaag ggtgggactc ggtgagcaac
     2461 atcgaggtgc tggtggcgct cgaacgcgag ttcgccgttc gattcaatac cggcgagatg
     2521 gctaccctgg ccaacgtcgg gcaactggtt gagctcatcg agctgagact gcggcggggc
     2581 gaggactgag accgtaagta tggaggcctt ctacttcggc ccgaacgaac ggcggctgtt
     2641 cggcgtgtat cagccgccag tcgtcgagag tcccgtggcg gccgcggcgg tgctctgcta
     2701 tccgttcctg caagagtacc ttcggtccca tcgcgcgttc catcggcttg ccggccttct
     2761 caccgctcgt gggtggcacg ttctccggtt cgactattcc cggtgtggtg attcctacga
     2821 tggtcctccg gatgtcgatg cctggagaaa ggatgtcaag ctcgcctgcg acgagctgcg
     2881 tgagcgggcg cagacgaggc gtctggcttt gatcgggctg cgccagggtg ccagtctcgc
     2941 cgcgtccgtg tcctgtgaca tgcagccgat ctccgatctg gtgatgtggg accccgtgat
     3001 cgacggtccc gcttttcggc aagagctgct cgggttccgc tattcagtga cactgccccc
     3061 gggcgcccgg cggaagctcg agcggctgga ccccggcgac cgcggcgtcg ggctcacgct
     3121 cggcccggac gtgctcgaag cgatcggtcg gttggaccgg acccagcaac tgaaggcgag
     3181 cgccgaccgg attctcgtgt tggacagcca gggagacgaa tcggcggtcg actttgcgcg
     3241 gcggctcgag aacctgcgtc ccaacgtcac tctgatccgc gtgtccgagc gtggcgtctg
     3301 ggtcgagcgc aagcggaagg acccccgtaa catctccgtg ccgacagtct ccctgcaggc
     3361 gatcgtcgac tggatgagta ggaccgaggg ttgagggagc acgcagtcgt cttcggtaac
     3421 gaatcacccc tggtcgggat tgtcaccgaa cccgactcca ggctgaacgg ggcaccggtc
     3481 tgcgtgctgc tcaacgccgg gctcggtcac cgcgtgggtc ccaaccgtct caatgtctcg
     3541 ttggcacgca agctcgccga cgtcggcctg acgtccctgc ggttcgactt ctctggaatc
     3601 ggcgacagtc ccgcctcgcc gtctctggac gatgccgaag agcgtggcgt caacgaaacc
     3661 atgcaggcga tggacttcct cgccgagtcc cttggtccgt caaggttcgt gccgatcggt
     3721 ctgtgctccg gagccaacgt cgcgttcgcg ctggcaaggc gcgacaagcg tgtggtcggg
     3781 gccgtcctca tcaacgcggc gacagttccc tccgcgcatt cggacgacca gatgaaggag
     3841 gcccgcacga gggccgaggc gcatcatcac gaggctcggc tcgccgattg gaagagctgg
     3901 ctgcgagtcc tgaccgcgcg gagtgacctg gctgccgtga cgcgttcaac gctgcggctg
     3961 gcccgccggg cggcaggcct gaaggaacag ccactacgtg agttcgacct cggagttctg
     4021 ccggagttgg atcggcgagg cgtcgaactc ctggtcatgt acaccgaggg ggacatcggt
     4081 ctcgagctcc tggccagtca cgtgggaccg ttcgagaacc tcgcgtccct ggagcgcgtc
     4141 cgcctcgaga ttctgcgcga gaccgatcac atcctgacgc cgctgtgggc tcaggctcag
     4201 gtcgaggaaa tgatgctcga ctggatgacc cggcgacttg gtgtgggtcg cgcagcgact
     4261 cgagccgagt cccacgcgct atgaggtcgt tgaggtcgtt tgctgaaggg cgctggttcg
     4321 tggtgcctcg accgctcgac gatccccggc tgcgtctgtt ctgttttccc tatgccggtg
     4381 gaggcgcatt cgcctttcgc aaatgggctg aacaggttcc tcgtgatgtc gaattctgcg
     4441 gcgtccagct tccggcgcgg gagggtcgct tcaaggagcc tgcctacacg cgactgaccg
     4501 atctggttcg agacctggcg gaggtcgttg gtcccgcaac tgacgtcccg tatgcgtttt
     4561 tcggccacag catgggagcg ctggtggcgt tcgcactcgc gagggaactg cggcgggtga
     4621 acgcgcgcgg acccgaactc ctcatggtgt ccggccgtcg cgcgccccag cgacccgatc
     4681 ccgatccgcc gatccatgcg ctgccaatgg ccgagtttct ccaggagctt cgagaactga
     4741 acggaacgcc ggaggcgatc tttcgagacg aggagctgct gcagttgctg gtgccgatcc
     4801 tgcgcgcgga tttcgagatc tgcgagacgt tcgagtatca gcaggagc
//
LOCUS       DBNGSH010000606         4203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_910200, whole genome shotgun sequence.
ACCESSION   DBNGSH010000606 DBNGSH010000000
VERSION     DBNGSH010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_910200"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="AC16CY_14040"
     CDS             <1..1466
                     /locus_tag="AC16CY_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354965.1"
                     /translation="YFDAMQSFPAVDDYRRGTRLELGDYDAGRGDDKFTLRVFGDARL
                     PIATTYADVYLWRNTATGKYFITMVNRLNAQDPNSPAHLEIKLLYGGAVHDQRYIVSV
                     PPGLGQRQGWYTVLRYNMTGRDSRLHRERRVWQDYKFYMWWAAGQDGKYGSADDVLAA
                     PPVNNNAVQTMCASCHLTGWERYQDKATGQYLVRAVNDPGGDMNIDDDPELDEINVGC
                     ESCHGPGSEHVANGGRSRYIVNPKYLSAERSSVVCGRCHDRRQGYGGPIVGYTQAVDE
                     TGALAGPGISRHELITKFTDPVKKGPTMRGGGREDNIWPDDVHSNKPHQQYADFLKSK
                     MYRNDRLLVTCSDCHDMHGNTPYPRWVIHDANDPRSPLCQRCHAVDVLSHMDAKLNAR
                     MKGLQTRCIDCHMPGTANTGGIAGDYGRMIKTPPYASAQEEEASAYWQGPLKSHVFDV
                     PLKTNVGVYGVPPGRAMPVPYTGACGTCHMVNELPFR"
     gene            complement(1513..1971)
                     /locus_tag="AC16CY_14045"
     CDS             complement(1513..1971)
                     /locus_tag="AC16CY_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3354966.1"
                     /translation="MTRVQDVMTEGVQTIAPTSSAEDAWELMQRNDFHHVVVTRGSRV
                     VGVLSHRDAGGRQGEVVRRGRTVEELMTSPVVTVEPTATVRKAANLMRGHSIGCVVVA
                     DRGRVVGIVTVADLLDLLGRGVDRPTPSEKRPALTHRTPHRKQSAATGVW"
     gene            2285..2542
                     /locus_tag="AC16CY_14050"
     CDS             2285..2542
                     /locus_tag="AC16CY_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354967.1"
                     /translation="MATATQTARVTEPGCWPAREALEENLREVRRVATTARHAAEDAV
                     AEAALNIRRHPLRAVGGAAVVAGIAGMLVGFGAGWYTREPR"
     gene            complement(2622..3140)
                     /locus_tag="AC16CY_14055"
     CDS             complement(2622..3140)
                     /locus_tag="AC16CY_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR3354968.1"
                     /translation="MTATQVTNATPAYDPTDNPTNCCPRFKPELWDGQELHFENKPFV
                     SASTVSLFHVPLNMGSVFARTWNAIKDAHAEDGGFLVLSHEDSAWHAEHLFAVSRPVP
                     GNDMVSLSGDFLTKVFEGPYEAAKRWADEMTHYVKERGHRLDTLYFFYTTCPKCAKYY
                     GKNYVVAIAKVK"
     gene            complement(3237..>4203)
                     /locus_tag="AC16CY_14060"
     CDS             complement(3237..>4203)
                     /locus_tag="AC16CY_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766882.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR3354969.1"
                     /translation="PYLRFVRGIVDSNDLPLNVSREILQQSRDVQTIRNASVKRVLSL
                     IEDLAEHHAERFATFWKEFGRVLKEGTADDAGNRERIAKLLRFASTKSDTDEQTVSLG
                     DYVGRMKEGQDAIYYVTADSFSAARNSPHLEIFRKLGVEVLLMYDRIDEWVVSLVTEF
                     DGKPLHSVARGGLDLGKIGGESEAKEQEKQTDEHKGLVERMQAVLKDRASSVRVTARL
                     TDSPSCLVSGEHGMSAHLERMLKAAGQNVPRSKPVLEINPRHPIVLRLTDESDERRFS
                     DWSHILFDQAVLAEGGQLDDPAAFVKRLNDVMLALGGGASKIWMP"
BASE COUNT          760 a         1377 c         1314 g          752 t
ORIGIN      
        1 agtatttcga tgccatgcaa tcgttcccgg ccgtggacga ctaccgtcga ggaacgcgcc
       61 tcgagctggg cgactacgat gcgggcaggg gagacgacaa gttcacgttg cgagtgttcg
      121 gcgacgcgcg cctgccgatt gcaaccacct acgccgatgt ctatctctgg aggaacacgg
      181 ccaccggcaa gtacttcatc acgatggtga accggctgaa tgcgcaggac cccaacagcc
      241 cggcacatct ggaaatcaag cttctctacg gcggtgcggt ccacgaccag cgctacatcg
      301 tctcggtccc tccggggctg ggccagcgtc agggctggta cacggtgctg cgatacaaca
      361 tgaccggccg agacagccgt ctccaccgcg aacggcgcgt ctggcaggac tacaagttct
      421 atatgtggtg ggccgcaggg caggacggga aatacggaag cgccgacgat gtgctggcgg
      481 caccgccagt caacaacaac gccgtacaaa cgatgtgcgc cagttgtcat ttgaccgggt
      541 gggagcgata tcaggacaag gcgacgggtc agtaccttgt ccgggcggtc aacgaccctg
      601 gcggcgacat gaacatcgac gacgatccgg agctcgacga gatcaacgtc ggctgcgaaa
      661 gctgccacgg tccgggatcg gagcatgtcg ccaatggcgg gcgctcgcgc tacatcgtca
      721 accctaaata cctctcggct gagcgctcga gcgtggtgtg cggccgctgt catgaccggc
      781 ggcaaggata cggggggcct atcgttggct atacgcaagc tgtcgatgag accggagcgc
      841 ttgccggtcc gggcatcagc cgtcacgagc tgattacgaa gttcaccgac cccgtcaaga
      901 aggggccgac catgcgggga ggcgggcgag aggacaacat ctggcccgat gacgtccact
      961 ccaacaagcc tcaccagcag tatgcggatt tcctcaagtc gaagatgtac cgcaacgatc
     1021 ggcttctggt gacgtgttcc gactgccatg acatgcacgg gaacacgccg tacccgcgct
     1081 gggtcattca cgatgccaac gatccacggt cgccgctgtg ccagcggtgt catgcggtgg
     1141 acgtgctgtc acacatggac gcgaagctca acgcgcggat gaaaggcctg cagacgcgct
     1201 gcatcgattg ccacatgccg ggaaccgcca acacgggcgg cattgccggt gactacggcc
     1261 ggatgatcaa gacgcctccg tacgccagcg cgcaggagga ggaggccagt gcctattggc
     1321 aaggccccct caagtcgcat gtgttcgacg tgcccttgaa gaccaacgtc ggcgtctacg
     1381 gagtgccgcc gggccgggcg atgcccgttc cgtacacggg cgcctgcggc acgtgtcaca
     1441 tggtgaacga gttgccgttc agataatcgt catccgtgac gcacctgata gagctggtgc
     1501 agcccagacc cttcaccaca cgcccgtcgc cgctgactgc tttcgatgcg gtgtccggtg
     1561 cgtgagcgct gggcgcttct ccgatggcgt cggacgatcg acgccgcggc cgagcagatc
     1621 gagcagatcg gcaacagtca cgatgcccac gacccgccca cggtctgcga cgaccacgca
     1681 tccgattgag tggccgcgca tcagattcgc cgcctttctc acggtggcgg tcggttcgac
     1741 cgtcacgacg ggactggtca tcagctcttc gaccgtccgg ccccttcgga ccacctcgcc
     1801 ctgccggccg ccagcgtcgc ggtgcgacag cactcccacc acccgggatc ctcgcgtgac
     1861 gaccacgtga tggaagtcat ttctctgcat caattcccac gcgtcttccg ccgaactcgt
     1921 cggggcgatg gtctgcacgc cttccgtcat gacatcctgg acacgcgtca cacaacctcc
     1981 ctcgaacaga cgtcatctcc cgctgctgcc cgctgacgca agagggattc caccaacgag
     2041 catgaagccc ggacgaaact gggccgattc ccgaggacca cagcgatcct gcgatgcgcg
     2101 tcgtgtggaa ttcttctcaa tgcgcgcgac tttttcgccg gcggcccgga tggccctgcc
     2161 agcacaaagc ctcacgatcg cgactcgcct cccgattcct tcgcgctacg tccttcctcc
     2221 atccggggac tacacgtctg ccgcgaggta cgtgaattgc tcgccttgtg acaggaggtg
     2281 agccatggcc actgccacac aaaccgcacg tgtgaccgaa ccaggttgct ggccggcacg
     2341 agaggcactc gaagagaacc tgcgcgaggt acgtcgtgtg gccaccaccg ctcggcacgc
     2401 ggcggaggac gcggtcgcgg aagccgcact gaatatcagg cgccatccac tccgcgccgt
     2461 cgggggcgcc gcggttgtcg caggcatcgc cggcatgctg gttggattcg gcgccggctg
     2521 gtacacgcgc gaacccaggt aaagggcggt catcgagggc cagagcgcca tcaccgagtc
     2581 ctcgccgcgg cggcgacgta cggcggggag tcggtgaagg ctcacttcac tttcgcgatg
     2641 gcgacgacgt agttcttgcc gtagtacttc gcgcacttcg gacacgtcgt gtagaagaaa
     2701 tacagcgtgt cgagcctgtg ccctcgctcc ttgacgtagt gtgtcatttc gtctgcccac
     2761 cgcttcgccg cctcgtacgg cccttcgaac accttggtca ggaagtcacc gctcaggctg
     2821 accatatcgt ttcctgggac tggccgcgac acggcaaaca agtgctctgc gtgccaggcc
     2881 gagtcctcat ggctcaagac caggaaacca ccgtcttctg cgtgagcgtc cttgatggcg
     2941 ttccatgtac gcgcaaacac ggagcccatg ttcagcggca cgtgaaacag actcaccgtg
     3001 gacgccgaga cgaacggctt gttctcgaag tgcagttcct gcccgtccca gagctccggc
     3061 ttgaaccgcg gacagcaatt ggtgggattg tcggtcggat cgtatgcggg cgtcgcgttg
     3121 gtcacttggg tcgcggtcat gtcacccctc cgcgttcgca gattgcacag agcggaccaa
     3181 cgctgcggcc ggcaacggct cttcgccgcg tgggcgccgc tgaggtgtaa cgactgtcag
     3241 ggcatccaga tcttcgacgc cccgccgccc agtgcgagca ttacgtcgtt caaccgcttg
     3301 acgaaggctg ccgggtcgtc gagttgaccg ccctcggcca gtaccgcctg gtcgaacagg
     3361 atgtggctcc agtcgctgaa gcggcgctcg tccgattcgt cggtcaaacg caggacaatc
     3421 gggtgccgcg ggttgatctc gaggacgggc ttggagcgag gaacgttctg gccggcggcc
     3481 ttcagcatcc gctcgaggtg ggcgctcatc ccgtgctcgc cgctcaccag gcacgacggc
     3541 gaatcggtga gccgcgcggt aacgcgcacc gaacttgcgc gatccttcag caccgcctgc
     3601 atgcgctcga cgaggccttt gtgttcgtcg gtctgcttct cctgttcctt cgcctcgctc
     3661 tcgccgccga tcttgccgag atccagcccg ccccgggcca ccgagtggag cggcttgccg
     3721 tcgaactcgg tgacgagcga caccacccat tcgtcgatgc ggtcgtacat cagcagcacc
     3781 tcgacgccga gcttgcggaa gatctcgagg tgcggactgt tccgggccgc ggagaagctg
     3841 tcggcggtca cgtagtagat ggcgtcctgt ccctccttca tccgccccac gtagtcgccg
     3901 agggagaccg tctgctcgtc ggtgtcgctc ttggtcgacg cgaagcgcag cagcttcgca
     3961 atccgctcgc ggttcccggc atcgtcggcc gtgccttcct tgagcacgcg gccgaactcc
     4021 ttccagaagg tcgcgaacct ctcggcatgg tgctcggcga ggtcttcgat caggctgagc
     4081 acgcgcttga ccgacgcgtt gcggatcgtc tgcacgtcgc gcgactgctg caggatctcg
     4141 cgcgacacgt tcagcggcag gtcgttgctg tcgacgatgc cgcggacgaa gcgcagatac
     4201 gga
//
LOCUS       DBNGSH010000607         2847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3626545, whole genome shotgun sequence.
ACCESSION   DBNGSH010000607 DBNGSH010000000
VERSION     DBNGSH010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3626545"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..697)
                     /locus_tag="AC16CY_14065"
     CDS             complement(62..697)
                     /locus_tag="AC16CY_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354970.1"
                     /translation="MKRPYKFGGGALVASGVLFAVVAYLEFETGPPPSNGAEILLWRD
                     SQALVLDFVSESLFFATVLLVPGTIALYQSLVDVDRTKAAAGCGIIAATIPVMAVMLI
                     IHGRLVYPIHGMRVDTPETAAFVIMVFYGGLHAVYLLLAVATIVLSLAMKRSAYATWV
                     AYLGFATAALDIIGSYPWAIGPVLTLVCELSFGGWFVAVGSQLSRMRGSAA"
     gene            941..1756
                     /locus_tag="AC16CY_14070"
     CDS             941..1756
                     /locus_tag="AC16CY_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354971.1"
                     /translation="MRTDRLALSAITWTLCLCLAAPAAAADLQEHTARAFDSYVTQAR
                     RAFLERIGGVAADGSKVQRGATLRDAEVTAGPAGEDGIIAVPGGLVHHWMGSTFIAGA
                     SLQDVLAVSYAYDEYHAVYKPVSASRLLGREGNTYRALLRIGESGAGLSAILDVTTRV
                     QYSYPHSRSVYSTSASDEIREVRNAGKTNEHSLSPGHDSGYLWRAATFTHFLQRDSGV
                     LVEMEAIGLSRQFPSLLRWIIEPVAQRLGRRSVERSLREFGEAVRARARQAMS"
     gene            complement(1796..2716)
                     /gene="ypfJ"
                     /locus_tag="AC16CY_14075"
     CDS             complement(1796..2716)
                     /gene="ypfJ"
                     /locus_tag="AC16CY_14075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR3354972.1"
                     /translation="MRTENQRRSDNFEDRGTGRGGGRGGGVPIQALFSIVRVLGFKGT
                     LLAGVVLAIGFFVLPAGLKQELLGALTGGGVAAPSTAGAGSVCDASQANFAACDFSRV
                     VLASTEDVWTQQFERGALPNYNSAPGAYRNPTLAVFARSVSTGGCGDATSDVGPFYCP
                     GDQKLYIDPSFYNVMEQRLRAPGDFAQAYVIAHEVGHHVQNLIGSTRLKMRGETSNQN
                     SVRVELQADCLAGVWGRTAQLSLQITDDDLGEALNAAHAIGDDTLGHSDERTFTHGSS
                     DQRKRWFRRGFDSGDARQCDTFAVRNSLEL"
BASE COUNT          544 a          892 c          931 g          480 t
ORIGIN      
        1 cgcatcgagc cgatgacggc gctcagacct gagtagaagg aaatccacac ggagctgggt
       61 ttcaggcggc actgcctcgc attctggaca gttgcgaccc taccgcaacg aaccatcccc
      121 caaaggacaa ctcacagacg agtgtcagaa cgggtccgat ggcccaggga taggacccaa
      181 tgatgtcgag cgcagccgtg gcgaacccaa ggtacgcgac ccacgtcgcg taggcactgc
      241 gcttcatcgc caagctcagg acaatcgtcg cgacggccag aagaagatag acggcgtgca
      301 ggccgccgta gaagaccatg atcacgaacg ctgcggtctc cggcgtatcg acgcgcatgc
      361 catgaatcgg atagacgagg cgtccgtgaa taatgagcat cacggccatg accggaatcg
      421 tcgccgcgat gattccgcag cctgccgccg ccttcgttct atcgacgtcc acgaggcttt
      481 gatacaaggc aattgttccc gggaccagga ggactgtcgc gaagaagaga gactcgctga
      541 cgaagtccag aacaagggcc tgcgaatccc gccacaggag aatctcggca ccgttcgaag
      601 ggggtggacc tgtctcgaac tccaggtacg ccaccacggc gaacaggacg ccactggcaa
      661 cgagtgctcc tcccccgaat ttgtaggggc gcttcatttc atgcggcatg gctagccggt
      721 aagaccgacg aagcaagcga ggtaccggtg cagttgagcc aggagggctg gtgacagcaa
      781 tggttcgagg ctgaattctc agcagtcagg aagggaatat tccccagccg gccgaaccag
      841 cgggagcggt gctcggagtc gaacagtcaa gattccaagt gccatctgcc cggacgctcg
      901 gcatggatcg tgctccgtcc ccacgcggga gagattccgc gtgagaacgg atcgccttgc
      961 tctaagcgcg atcacatgga cgctgtgcct gtgcctggcc gcccccgccg ctgctgcgga
     1021 cctgcaggag cacaccgctc gcgctttcga cagttatgtg acgcaagcca ggcgcgcgtt
     1081 cctcgaacgg atcggcggcg tggcggccga cggttcgaaa gtacagcgag gggcgacact
     1141 gcgcgatgcc gaggtgacgg caggcccggc gggcgaagac ggcatcatcg cggtgccggg
     1201 cggcctcgtg catcactgga tgggatcgac cttcatcgcg ggcgcgtcgc tgcaggatgt
     1261 gctggcggtc tcgtacgcct acgacgagta tcacgccgtc tacaagccgg tcagcgcgtc
     1321 caggctgctc ggccgcgagg gaaacaccta tcgtgcgctg ctgcggatcg gggaatcggg
     1381 cgccggcctg agcgcaatcc tcgacgtgac cacgcgcgtg cagtactcct atccgcacag
     1441 ccgcagcgtg tacagcacct cggcgtccga tgagatccgc gaggttcgga atgcggggaa
     1501 gacgaacgaa cacagcctct cacccggtca cgacagcgga tacttgtggc gtgcggcgac
     1561 gttcacccat ttcctgcagc gggactcggg cgtgctcgtc gagatggaag cgatcggcct
     1621 gagcaggcaa ttcccgtcgc tgctgcggtg gatcatcgag cccgtcgcgc agcggctggg
     1681 tcggaggagc gtcgagcgct cgctccggga attcggcgag gcggtgcggg cccgcgcgcg
     1741 acaggcgatg tcctgacaac gggacgccgc gagtgtcgct cgcgtgctgc ctgggtcaga
     1801 gctcgagaga gttgcgaacg gcaaacgtgt cgcactggcg ggcgtcgccg ctgtcgaaac
     1861 cgcggcggaa ccatcgcttg cgctgatcgc tcgagccgtg cgtgaatgtc ctctcgtcgg
     1921 aatggccgag ggtgtcgtcg ccgatcgcgt gcgcggcgtt gagtgcttca cccagatcgt
     1981 cgtccgtgat ctggagtgac agctgcgccg tgcgacccca gacacccgca aggcaatccg
     2041 cctgcagctc gactcgcacc gagttctgat tcgaggtctc gcccctcatt ttcagcctgg
     2101 tggagccgat caggttttgc acgtggtgac cgacctcgtg tgcgatcaca tacgcctggg
     2161 cgaagtcgcc gggcgcgcgc aggcgctgtt ccatgacgtt gtagaagctc ggatcgatgt
     2221 agagcttctg atcccccggg caatagaatg ggccgacgtc ggaggtcgca tcgccgcatc
     2281 cgccggtgga cacgctcctg gcaaacacgg cgagcgtggg atttctgtag gcgcccggag
     2341 cgctgttgta gttcggcagc gccccgcgtt cgaattgctg cgtccacacg tcctcggtcg
     2401 acgcgaggac gacgcgtgag aaatcgcacg cggcgaagtt cgcctgcgac gcgtcgcaca
     2461 cgctgccggc gcctgccgtc gaaggcgctg ccacgccgcc gcccgtcaga gctcccagga
     2521 gttcctgctt gagccccgca ggcaacacaa agaaccctat cgcgagcacg acccctgcga
     2581 gcagggtgcc cttgaatcca agaaccctca cgattgagaa gagcgcctga atcgggactc
     2641 caccgccacg tccaccaccg cggccggtgc cgcgatcttc gaagttgtcg gaccggcgtt
     2701 gattttcagt acgcatgacc gccttggtgt ggctggatgg gcgagatagc tggatagtat
     2761 gtcgtatacc gacccgacgc actcgtgaga gctctcctct acctcaggcg tacgccgtca
     2821 gcgggattgc tgctggtgca gctgatc
//
LOCUS       DBNGSH010000608         2721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2607920, whole genome shotgun sequence.
ACCESSION   DBNGSH010000608 DBNGSH010000000
VERSION     DBNGSH010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2607920"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="AC16CY_14080"
     CDS             complement(<1..703)
                     /locus_tag="AC16CY_14080"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3354973.1"
                     /translation="MIERRSFLKATTAATGGLLLGYYLPESGSAVVEAQFRPGGGGAI
                     TPHAFVRVGADDVVTLIIHKPDNGQGTETSIAMLLAEELECDWSRIRTEFAPIDPALY
                     GGPLQGTFGSMAIRTSYEPMRRTGAAAREMLVQAAAARWGVEPQQCRAENGQVLNLVT
                     KARLTYGSVAEAASKLPVPANITLKDPAQFRLIGKPTRRLDTPAKVSGRATFGIDVTL
                     PGMLHAVVARCPVFGG"
     gene            complement(719..1159)
                     /locus_tag="AC16CY_14085"
     CDS             complement(719..1159)
                     /locus_tag="AC16CY_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3354974.1"
                     /translation="MRTLAMSMATAALLSVATAPTDAHHAFGGEFDATKPVLLKGAIV
                     KVEWVNPHTWIHMEVKKLDGTSEVWMIEGGSPNSLLRLGIRKDSLPVGTEIVVDGYQA
                     RDHTLKRANGRNITYPDGRKLFFGSSGTGAPRDGADPSEQKPGK"
     gene            complement(1187..2341)
                     /locus_tag="AC16CY_14090"
     CDS             complement(1187..2341)
                     /locus_tag="AC16CY_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354975.1"
                     /translation="MRTSSRTLVLTALLGTLVVLAVPLSERVGTQGATPYSPPRLADG
                     RPDLQGTYDLATLTPVERPAGMNATLSREEVAKLEKTVAAVLALGARPISGDRAAPPA
                     GGDGSRGAAGNVGGYNTFWLDPGSRYTMVNGEARTSIVVDPPDGRLPPLTPAARKRLL
                     GLLGGLLTRGGAENDPGLDPTPGAYDDPERRGLGERCLLGFGSTAGPPALPNYFYNNL
                     HKIVQTPNAVLILTEMVHDARIVRMNAEHLPRHIRKWMGDSVGRWEGDTLVVDTTNFN
                     SDQYRYVVEASPGRSLVLVDSIGAKEPDRLNGPSRDLRVIERFTRVADNALLYRFTVE
                     DPGTWEKPWTGEYTWPSTDQPIYEYACHEANYALENVLRGARQRDAAAKK"
     gene            complement(2369..>2721)
                     /locus_tag="AC16CY_14095"
     CDS             complement(2369..>2721)
                     /locus_tag="AC16CY_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354976.1"
                     /translation="DPGPAPVESWMGQSVGRWDGDTFVVDVTGFNDQTWFDRAGNFHS
                     DALHVVERYTRRNQDVMTYEATIDDPKVFTRPWKMSMALYRRQERNAQLMDFKCVEFV
                     EELMFGQWRKNPLK"
BASE COUNT          425 a          931 c          872 g          493 t
ORIGIN      
        1 tgccgccaaa cacgggacag cgcgccacca ccgcgtgcag catgcccggc agcgtgacgt
       61 cgatcccgaa cgtcgcgcgg ccgctcacct tcgcaggggt atcgaggcgc cgcgtgggtt
      121 tcccgatcag acggaactgc gcggggtcct tgagcgtgat gttggccggc accgggagct
      181 tcgaggcggc ctccgccacg ctgccgtagg tcagccgcgc cttcgtgacg aggttgagga
      241 cctggccgtt ctcggcgcgg cactgttgcg gctcgacgcc ccaccgggcc gccgcggcct
      301 gcacgagcat ctcgcgcgca gcggctcccg ttctccgcat cggctcgtac gaggtccgga
      361 tcgccatgct cccgaacgtg ccttgaagag gaccgccgta cagcgccgga tcgattggcg
      421 cgaactccgt cctgatcctg gaccagtcgc actcgagctc ttcggccagc agcatcgcga
      481 ttgacgtctc ggtcccctgc ccgttgtccg gcttgtgaat gatcagcgtc acgacgtcgt
      541 cggcgccgac gcggacgaag gcgtgcggcg tgatcgcgcc gccgccgccg ggacgaaact
      601 gcgcttcgac gaccgcgctg ccgctctcgg gcaggtagta gccgaggaga aggccgcccg
      661 tggccgcggt ggtcgccttg aggaaactgc gacgttcgat catgtgagtg ctcccgcgct
      721 acttcccagg cttttgctcg ctgggatcgg cgccgtcgcg cggcgctccc gttcccgacg
      781 atccgaagaa gagcttcctg ccgtcaggat aggtgatgtt gcggccgttc gcccgcttga
      841 gcgtgtggtc gcgagcctga tacccgtcaa cgacgatctc ggtgcccacg ggcagggaat
      901 ccttccgaat cccgagccga agcagcgagt tcggactccc tccctcgatc atccacacct
      961 cgctcgtgcc gtccagtttc ttcacttcca tgtggatcca cgtatgaggg ttgacccact
     1021 cgaccttcac gatcgcgccc ttcaggagga ccggcttggt cgcgtcgaac tcgccgccga
     1081 aggcgtgatg ggcgtcggtg ggcgccgtgg ccaccgacag aagcgccgcg gtggccatgg
     1141 acatagcgag tgtccgcatc gttgtccctt tcatcgcagt cggcccctac ttcttcgccg
     1201 cggcgtcccg ctgacgggcg cctcgcagca cgttctcgag cgcgtaattg gcctcgtggc
     1261 acgcgtactc gtagatcggc tggtccgtgg agggccaggt gtattcgccc gtccacggct
     1321 tttcccacgt gcccggatcc tcgaccgtaa aacgatagag caacgcattg tctgccacgc
     1381 gcgtgaagcg ctcgatcacg cgcaggtcgc ggctgggccc gttcaaccga tccggctcct
     1441 tggcaccgat gctgtccacg agcacgagac tgcggccggg agacgcttcg acgacatagc
     1501 gatattgatc gctgttgaaa ttggtggtgt cgacgacgag cgtgtcacct tcccaacggc
     1561 ccaccgaatc tcccatccac ttgcggatgt gccttggaag gtgctcggcg ttcatccgca
     1621 cgatacgagc gtcgtgcacc atttcggtga ggatgagcac ggcattcggg gtctgcacga
     1681 tcttgtggag gttgttgtag aagtaattcg gcagcgcagg aggccccgcc gtcgatccga
     1741 atccaagcag acaccgctcg ccgagcccgc gtcgctcggg gtcgtcgtac gcgccaggtg
     1801 tggggtcgag gccgggatcg ttctcggcgc ctccccgtgt cagcaaacca cccagcaggc
     1861 ccaacaggcg tttccgtgcg gctggcgtca gcggcggcaa acggccatcg ggaggatcga
     1921 cgacgatcga ggtgcgcgct tccccattga ccatcgtgta gcgggacccg ggatcgagcc
     1981 agaaggtgtt gtagccgccg acgtttccgg cggcaccgcg cgagccatcg ccgcccgcgg
     2041 gcggcgcagc gcgatcgccg ctgatcggcc gcgctccgag cgccagcacc gcggcaacgg
     2101 tcttttcgag cttggcaacc tcctcgcggc tcagcgtcgc gttcatcccg gccggccgct
     2161 cgacgggcgt cagcgttgcc agatcgtagg tcccctggag gtccggccgt ccgtcggcca
     2221 ggcgaggggg cgaatacggc gtcgctccct gcgtgccgac gcgctccgac aacggaacgg
     2281 ccagaacgac gagcgtcccg agcagggccg tcaggaccag cgttcgagat gatgtgcgca
     2341 tgcgtgctcc cttgctacca gccaccagct atttgagcgg attcttccgc cactggccga
     2401 acatcagctc ctcgacgaac tccacgcact tgaagtccat cagctgcgcg ttccgttcct
     2461 ggcgcctgta gagcgccatg ctcatcttcc agggccgtgt gaatactttc ggatcgtcga
     2521 tcgtcgcctc gtaggtcatg acgtcctgat tccggcgcgt gtagcgctcg accacgtgca
     2581 gggcgtcgct gtgaaagttg cccgcgcgat cgaaccaggt ctgatcgttg aagccggtga
     2641 catcgacgac gaacgtatcg ccatcccatc gccccaccga ctgccccatc cacgactcga
     2701 cgggagcggg ccccggatcc t
//
LOCUS       DBNGSH010000609         3951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1589290, whole genome shotgun sequence.
ACCESSION   DBNGSH010000609 DBNGSH010000000
VERSION     DBNGSH010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1589290"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC16CY_14100"
     CDS             <1..219
                     /locus_tag="AC16CY_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HIR3354977.1"
                     /translation="LRHALLEQPEIFVGTLTEKMLTYGLGRGLQHYDMPVVRAIVRDA
                     AQRDYRFSSVIVGIVSSAPFQMREKAGT"
     gene            347..2062
                     /locus_tag="AC16CY_14105"
     CDS             347..2062
                     /locus_tag="AC16CY_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016395019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354978.1"
                     /translation="MPNRQAFEWFKTTFHTPIETAIAGTPFSLDLIAALAAQETGSIW
                     APLRNKLDLATLLETCVGDVIDATGGRQAFPRTRQDLEAVARGDEMFRIAHDALAAMA
                     VHVPVYAKFAANPNRFCHGYGIFQYDIQFFKEDPDYFLSRDWRHFEKSLGKAVGELKN
                     AQRRIPHLRGLTSLTDLEQVHVAIAYNAGSFRPAKGLQQGFKPKDGKFYGEMVFDFLR
                     LSQTVSIPATPSLLPEPAPGAAPLARPTPIVSTGAVLEVDVRETPLRLRREPRVSRPD
                     PNENVITRLPDGHRVRLVSGTLGEKFVEVETSLDGARFRGFAASEFLVRVPGARDVPV
                     VVPAPAPPTSGVVAVFAPRREGTVTTRRQPATAHSLNEPDRPGRTGSTPDALRAEIDG
                     IIDYLNVEKTSHARYQPADGRTFCNIYAHDFCALAGVYLPRVGWTPGAIERLARGENV
                     EPRLGGTIEEQRANDLFRWLRDFGTRFGWRQTSTLTKLQTEADVGAIGLIVARRVDDG
                     RPGHITIVVPENADARARRDSGTGDVTAALQSQAGARNFSRDTGKVDWFKGTAFAEHA
                     FWLHA"
     gene            complement(2096..2839)
                     /locus_tag="AC16CY_14110"
     CDS             complement(2096..2839)
                     /locus_tag="AC16CY_14110"
                     /inference="COORDINATES: protein motif:HMM:NF025086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR3354979.1"
                     /translation="MARLIAAIVLLSAMALPAAGQNFLGRFFRAQPVAVEYDSYFAFT
                     RIRYGTRVGTNGGWEHDYPAADRNFSAILDYISNMRVHLNDSNIFDLDDPRIFENPVI
                     YMSEPGYWTTDEAEAKSLREYLLKGGFIIFDDFDGDPEWRNLVRQMKVVLPEHDFIRL
                     DVSHPIFQSFYSLRTLNVPHPMLPGIEPVFYGLFDDNRTGGRMLAIANFNNDIGDYWE
                     WSAEGLYGEAGTADAYRLGVNYLVYAMTH"
     gene            2906..3349
                     /locus_tag="AC16CY_14115"
     CDS             2906..3349
                     /locus_tag="AC16CY_14115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3354980.1"
                     /translation="MCTGVAVTMCTTVFAVCARTPRPVAASIKPCGGEGRGRYGGPGP
                     VPVTGLLTLQCMTVKELILEAYSSREPAPAMRPSVEGGPAWIRSDRYTITATAEGKPV
                     LARVRGAMLRRLLEDRFDLRLHHEKRELAPAGVLVIDAVDRPSGH"
     gene            3407..>3951
                     /locus_tag="AC16CY_14120"
     CDS             3407..>3951
                     /locus_tag="AC16CY_14120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3354981.1"
                     /translation="MPKRFVSIGLLCVTAALVYLCVIAIASTASSPAHAQAPSFDVAT
                     IKLSDAPPNAGALLNFPSPQRMSITNFSLRMLIGSAFGPELGQGYQVSGGPDWLEKDR
                     FVVQGQASGTATRADMTAMLRTLLVERFGLKYHVENKDVDAYALVLDRADGKLGPKLQ
                     KWDGTCNGKQPPPAQPNSTGPR"
BASE COUNT          680 a         1349 c         1287 g          635 t
ORIGIN      
        1 ctgcgacacg cgctgctcga gcagcccgag atcttcgtcg gcacgctgac ggagaagatg
       61 ctgacctacg gcctcggccg gggactccag cactacgaca tgcccgtcgt gcgcgccatc
      121 gtccgcgacg ccgcgcagcg ggactaccgc ttctcgtccg tgatcgtcgg gatcgtcagc
      181 agcgcgccgt ttcagatgcg ggagaaagcc ggtacgtagc gtccggcttt cctggcctga
      241 gcgaagtcga aggcagccgg accgagggtg ttcaatcgcg cacccgccgt cgttgttata
      301 gacgcacccc ttatccacct aagactaaac cgctcgagta cgtgccatgc ccaaccgaca
      361 agcgttcgaa tggttcaaga ccaccttcca cacgccgatc gagacggcga ttgccggcac
      421 gccgttcagc ctcgacctga tcgccgcgct cgctgcgcag gagaccggat cgatctgggc
      481 cccgctgcgc aacaagctcg accttgcgac gctgctcgag acctgcgtcg gcgacgtgat
      541 cgatgccact ggcggccgcc aggcgttccc gcgcaccaga caggacctcg aggcggtggc
      601 gcgcggcgac gagatgttcc ggatcgcgca cgacgcgctg gcggcgatgg ccgtccacgt
      661 gcccgtgtac gcgaagttcg cggccaaccc caaccggttc tgccatggat acggcatctt
      721 tcagtacgac attcagttct tcaaggagga ccccgactac tttctgagtc gcgactggcg
      781 ccatttcgag aagtccctcg gcaaggcggt cggcgagttg aagaacgccc agcggcgaat
      841 cccgcacctc agggggctca cgagcctgac cgatctcgag caggtgcacg tcgccattgc
      901 ctacaacgcc ggatcgttca ggcctgcaaa gggtcttcaa cagggattca agccgaagga
      961 cgggaagttc tacggggaga tggtcttcga cttcctgcgc ctctcgcaga ccgtttcgat
     1021 tcccgccacg ccgtcgctgc tccccgaacc ggcgcctggc gcggcgcctc tcgcgcgccc
     1081 gactccgatc gtctcgacgg gcgccgtgct cgaggtggac gtcagggaaa cgccgctgcg
     1141 gctgcgccgc gagccgcgcg tcagcagacc cgatcccaac gagaacgtca tcacgcggct
     1201 gcccgacggc catcgcgtcc gcctcgtctc cggcacgctc ggtgagaaat tcgtggaggt
     1261 cgagaccagc ctcgacggtg cgcgattccg cggtttcgcc gcctcggagt tcctcgtgcg
     1321 tgtcccgggc gcgcgcgacg ttccagtcgt cgtgcccgcc ccggcgccgc cgaccagcgg
     1381 cgtcgtcgcg gtcttcgcgc cgcgccgcga agggacggtc acgacccgca ggcagccggc
     1441 gaccgcgcac tcgctgaacg agcccgatcg acccggccgc accggctcga cacccgacgc
     1501 gctgcgcgcg gagatcgacg gcatcatcga ctacttgaat gtcgagaaaa cctcgcacgc
     1561 gcgctatcag ccggccgacg gccgtacgtt ctgcaacatc tacgcgcacg acttctgcgc
     1621 gctcgccgga gtctatctgc cacgcgtcgg gtggacgccg ggcgcgatcg agcgccttgc
     1681 ccggggcgaa aacgtcgagc cgaggctcgg cggcaccatc gaggagcagc gcgcgaacga
     1741 tctgttccgc tggctccgcg acttcggcac gaggttcggg tggcggcaga cgagcacgct
     1801 gaccaagctt cagaccgaag ccgacgtcgg cgccatcggc ctcatcgtcg cgcgcagagt
     1861 ggacgacggg aggcccggac acatcacgat cgtggtgccc gagaacgccg acgcgagggc
     1921 caggcgggac agcgggaccg gcgacgtcac ggccgcgctg cagagtcagg ccggcgcgag
     1981 gaacttctca cgcgacacgg ggaaggtgga ctggttcaag ggcaccgcct tcgcggagca
     2041 tgcgttctgg ctccacgcat aaaggcgtgg aggcggcgtg tgaaggccca caccgtcagt
     2101 gcgtcattgc gtacaccagg taattgacgc caagccgata ggcgtcggcc gtgcctgcct
     2161 cgccgtacag cccttcagcc gaccactccc agtagtcgcc gatgtcgttg ttgaaattcg
     2221 cgatggccag catgcggccg cccgtccggt tgtcgtcgaa cagcccgtag aacaccggct
     2281 cgattcccgg cagcatcggg tgaggaacgt tgagcgtccg gaggctgtag aaggactgga
     2341 agatcggatg gctcacgtcg agccggatga aatcgtgttc cggcagcacg accttcatct
     2401 gcctgacgag gttgcgccat tccgggtcgc cgtcgaagtc gtcgaagatg atgaacccgc
     2461 ccttcaggag atactcgcgc aggctcttcg cctccgcctc gtcggtcgtc cagtagccgg
     2521 gctcggacat gtagatgacc gggttctcga agatgcgggg gtcatcgaga tcgaagatgt
     2581 tcgagtcgtt caggtgcacg cgcatgttgc tgatgtagtc gaggatggcc gagaagttgc
     2641 ggtcggccgc ggggtagtcg tgctcccacc cgccattggt tcccacccgg gtgccgtagc
     2701 ggatgcgcgt gaaggcgaag tagctgtcgt actcgaccgc aaccggctgc gctcgaaaga
     2761 agcggccaag aaagttctga ccggcggccg ggagcgccat cgccgacagc agcacgatgg
     2821 cggcaatcag tctcgccata gctccccagt ctatcccggg aacctttcgg cgcacgcgcg
     2881 ggaatagccc gctatgaggc gagtcgtgtg caccggggtc gccgtcacca tgtgcacgac
     2941 ggtgttcgct gtctgcgcgc ggacaccgcg gccggtcgcc gcgtcgatca agccgtgcgg
     3001 cggcgagggc cgcggccgct acggcggccc gggcccggtg cccgtgacgg gactactgac
     3061 attgcagtgc atgaccgtca aggagttgat cctcgaggcg tattcctcgc gtgagccggc
     3121 gccggcgatg cggcccagcg tcgaaggcgg gcctgcatgg atcagatcag atcggtacac
     3181 catcaccgcc accgcggagg gcaagccggt gctcgcgcgg gtccgcggcg ccatgcttcg
     3241 ccggctgctc gaagaccgct tcgacctcag gctgcatcac gagaagaggg aactggcgcc
     3301 cgcaggcgtc ctcgtcatcg acgccgtcga ccgaccctcc gggcactgac gatcctgtgg
     3361 aaccttcttt ctcacgcccg ccaataacgg ggcaggagaa tttgcgatgc ccaagagatt
     3421 cgtctccatc ggcctgctgt gcgtgacggc tgcgctggtc tacctgtgcg tgattgccat
     3481 cgcctccacg gcgtcgagcc cggcgcacgc gcaagccccc agcttcgacg tcgcgaccat
     3541 caagctgtcc gacgcgccgc ccaacgccgg cgcgctgctc aacttcccgt ctccgcagcg
     3601 catgtcgatc accaacttct cgctgcgaat gctgatcggc tccgccttcg gacccgagct
     3661 cggccagggc tatcaggtct cgggtgggcc cgactggctc gaaaaggacc gtttcgtcgt
     3721 ccagggacag gcgtccggta ccgctacgcg cgccgacatg actgcgatgc tgcgcacgct
     3781 gctcgtcgag cgtttcggcc tgaagtatca cgtcgaaaac aaggacgtgg acgcctacgc
     3841 gctcgtgctg gaccgcgccg acggcaagct cgggccgaag ctccagaagt gggacggcac
     3901 ctgcaacggc aagcagccgc cgccggcaca gccgaattcc accggcccgc g
//
LOCUS       DBNGSH010000610         2309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4305764, whole genome shotgun sequence.
ACCESSION   DBNGSH010000610 DBNGSH010000000
VERSION     DBNGSH010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4305764"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="AC16CY_14125"
     CDS             <1..623
                     /locus_tag="AC16CY_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354982.1"
                     /translation="AGARDLTADLVIYLVPIYGIAARSATMGDTFLALPVKTVVLPEA
                     VVMALGVAAVLATGVWVVRSAASFARGTLNVQYAGFVLSHVAIFLTAYILIDDANVGW
                     LAINVWHNFQYVLVVWMANARKYSGEAAPQASLMARISQPHRVAAYFTICLAISTLVY
                     VVLGRFVAAFGGGLALTLGIYMGINFHHYIVDALIWKRRRAVPQAA"
     gene            788..1477
                     /locus_tag="AC16CY_14130"
     CDS             788..1477
                     /locus_tag="AC16CY_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354983.1"
                     /translation="MFRSLILVAALVSSVTAVQSGRQVPAALASAARGRPIEFAMLLA
                     HASIPVGLEVYGSDRDPRTMPDWRSIRGDEMVPLAVVIAAFNESHGDYGAELMDGVLV
                     IRPLARRAPFLDQPLMVSDLEVTGVETALRKMFKPIDPTLDAGGGILGSRSVTPEEAG
                     DFMTVRFTPGKTVLQTLNELAIQTGHGWKVVTTGSEEKKGASQIVFAGFLHRRSITSG
                     IPIRPIRPQTP"
     gene            complement(1645..1851)
                     /locus_tag="AC16CY_14135"
     CDS             complement(1645..1851)
                     /locus_tag="AC16CY_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354984.1"
                     /translation="MNVIPIAPERVDSMFDEVEQWAPRMDFADACAVVLVRSYEHAFV
                     LTTDFRDFSTYRVPFAAPEGAFHV"
BASE COUNT          377 a          734 c          778 g          420 t
ORIGIN      
        1 aggccggggc ccgcgacctg acggccgacc tcgtgatcta cctcgtgccc atctacggca
       61 tcgccgcccg gtcggcgacg atgggggaca cgttcctcgc gcttccggtg aagacggtcg
      121 tcctgcccga ggcagtcgtg atggcgctcg gagtggcggc ggtgctggcg acgggcgtct
      181 gggtggtccg gagcgcggcc tccttcgcac gcggcacgct gaacgttcaa tacgcgggct
      241 tcgtcctctc gcacgtcgcg atcttcctca cggcctacat cctgatcgac gatgccaatg
      301 tcgggtggct ggcgatcaac gtctggcaca acttccagta cgtcctcgtg gtgtggatgg
      361 cgaacgcgag gaagtattcg ggagaagcgg cgcctcaggc gtcgcttatg gcgaggatca
      421 gccagccgca tcgcgtggcc gcgtacttca cgatctgcct ggcgatctcg actctcgtgt
      481 atgtcgtcct cggccggttc gtcgccgcct tcggcggcgg actcgcgctg acgctcggca
      541 tctacatggg catcaacttc caccactaca tcgtcgacgc cctcatctgg aagcgccggc
      601 gcgcggtgcc gcaggcggcg tagacgtagt gtccggcttc agccggaccg gtgcgtagtg
      661 tccgctttca gcttctttct gcccagccgt acaattgcaa ccggtgaggg ctatgtttcg
      721 gggaacggcc ttctggacga ctaagtcgct gggaggtcgc gcccgggacc cttgctcgac
      781 ctcgaccatg ttcagaagcc ttattctcgt cgcggcgttg gtctcatcgg tcactgccgt
      841 gcagtccggg cggcaggtac ctgcggcatt ggcgtctgcg gcgcgcgggc gcccgatcga
      901 gtttgccatg cttctcgccc atgcctccat ccccgtcggg ctggaggtgt acgggtccga
      961 cagggatcca cggacgatgc cagattggag gagtatccgc ggcgacgaga tggttccgct
     1021 cgccgtcgtg atagccgcgt tcaacgagag ccacggggac tacggcgcgg aactcatgga
     1081 tggcgtcctg gtcattcgtc ccctggcgag gcgcgctccg ttcctcgacc agcccttgat
     1141 ggtgagcgac ctggaggtga ccggcgtcga aactgcgctg aggaagatgt tcaagccgat
     1201 cgacccgacc ttggacgctg gtgggggaat tctgggcagc cggtctgtca cgccggagga
     1261 ggccggggac ttcatgaccg tcaggttcac gcccggtaag acggttctcc agactctgaa
     1321 cgagcttgcg atccagacgg gccacggctg gaaggtcgtg acgaccggct ccgaagagaa
     1381 gaaaggagct tcacaaatcg tgtttgccgg attcctgcac cgtcgctcga tcacgtcggg
     1441 cataccgata cgaccgatac ggcctcagac cccgtaggtg ccattcggac atcgggccac
     1501 gggttcgggc ctcgggctac tggctacggg cttcgggctg ccgtggacat agtgtccggc
     1561 ttccttggac gccgacttgt ccgccaaagc gccgcgccgc gacggcggaa ggaccgtgcg
     1621 cgaaggcggc agccggacct gccgtcacac gtggaacgcg ccttcgggag ccgcgaacgg
     1681 cacgcggtag gttgaaaagt cgcggaaatc ggtagtgagg acgaacgcgt gctcgtagct
     1741 gcgcacgagc acgacggcgc aggcgtcggc gaaatccatg cggggcgccc actgctcgac
     1801 ttcgtcgaac atggagtcga ctcgctccgg cgcgatcgga atgacgttca tacgtgccac
     1861 cagccgccgg agtgcccgga ccgccgccgg atgattctcc aggccgtgcc gggcttcggc
     1921 aatcgccgcc tcacaggtga tgaaccgcgc ccgggaccgc gcgtaaagcg cgcacagccc
     1981 acgcgtggtc gatgtcgccc cggtcgatgg cggccttaag gggtcccggc gtcgacgacg
     2041 acggtcctgc gcagcgaggc gtcggcggac tttctcacgc aggcggctat ggttccgggg
     2101 cagcgtcacg gcgccaaaga cgccatcgag gcccgcgaag ggatcgtcgc ggttgcgccc
     2161 cggcgttcga agacctagtt gtagtttcca ggtaggttgt tcactcggag tcccttggga
     2221 agaatgggag ttaccacaca accgttcttc agcgaggagg ccccgagtga agcttcatcg
     2281 taacgccaag acgactccgt tgacccgcg
//
LOCUS       DBNGSH010000611         1846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_61343, whole genome shotgun sequence.
ACCESSION   DBNGSH010000611 DBNGSH010000000
VERSION     DBNGSH010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_61343"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="AC16CY_14140"
     CDS             complement(<1..982)
                     /locus_tag="AC16CY_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3354985.1"
                     /translation="MIKKTIVLLAVAGTIVAVGFGVARGLRAGIEALAEAGETAVPTT
                     KVRRADVLIEVSSRGELQGGGATDLIVPRAGSAELPITFLRGTGDRVEAGDIVADFDA
                     SGQQYLLVEAEADLEEAQQQLLKAEADVFVAQEKARLEVVTTKADLDVSELDQLENRF
                     LGSVQQRKNEIALEQARNRHEQAKLDLAHAEAKKATGINAQKSAVVEAQAKADAARKT
                     IAGMTLRAPSAGFVQVAENTNGLSVIFSGMQIPTFQLGDTARPGQTVARIPNMSQWEV
                     STQIPETDRAFLAVGQRAIVRPKAMPGREFKGHISLVGGSAGSAWNRTFNC"
     gene            complement(979..>1846)
                     /locus_tag="AC16CY_14145"
     CDS             complement(979..>1846)
                     /locus_tag="AC16CY_14145"
                     /inference="COORDINATES: protein motif:HMM:NF052764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3354986.1"
                     /translation="LAVEIAQLDTKDSEFVARLDAERGRIDLRVAEQKLRQLEAEVAQ
                     RAASRESKIASLQRQLAAAEGWVRIVESRISQMEISAPISGFALYTANRSSLSSQLSG
                     GAEQPYRVGNPVASGMNLATIPDLSSLLIDATVEEIDRGRMRIGDEVIVRVDALPDVS
                     IQAKLTGISPMAEMSLDSRGRSFHAYAALGDKSDSRVRPGMNGTLDIVTRRIPGATII
                     PAKALFTRNGKPTVHLVDGDEYRTVEVEVLARNSDEIAVKGVESGARVTLVDPLAEGS
                     GDAGGGTAGNVK"
BASE COUNT          258 a          655 c          598 g          335 t
ORIGIN      
        1 gacagttgaa cgtcctgttc cacgcgcttc ccgcggaccc gccgacgagg ctgatgtggc
       61 ccttgaactc gcgccccggc atcgccttgg ggcgcacgat cgcgcgctgg cccacggcga
      121 ggaacgcacg gtcggtctcc gggatctgcg tgctcacctc ccactggctc atgttcggga
      181 tcctcgccac ggtctggccg ggacgagccg tgtcgccgag ctgaaacgtc gggatctgca
      241 tgccgctgaa gatgacgctg agaccgttgg tgttctcggc cacttgaacg aagccggccg
      301 agggcgcgcg cagcgtcatg ccggcgatcg tcttacgggc cgcatcggcc ttggcctgcg
      361 cctccaccac agccgacttc tgcgcgttga tgccggtcgc tttcttcgcc tcggcgtggg
      421 ccagatccag cttcgcctgc tcgtgccggt tccgcgcctg ctcgagggcg atctcgttct
      481 tgcgctgctg aaccgaaccc aggaaccggt tctcgagctg gtccagctcg gagacgtcga
      541 ggtcggcctt ggtggtcacg acctcgaggc gggccttttc ctgcgcaacg aacacgtccg
      601 cttcggcctt gagcagctgc tgctgcgctt cttcgaggtc ggcttcggct tcgacgagca
      661 ggtactgctg cccgctcgcg tcgaagtccg cgacgatgtc gcccgcctcg acgcgatccc
      721 ccgtgcctcg caggaacgtg atcggcagct ccgcggatcc ggccctcggc acgatcaggt
      781 cggtcgcgcc gccgccctgc agctcaccac gcgaggacac ttcgatgagg acgtccgcgc
      841 ggcggacctt cgtggtgggc acggcggtct caccggcctc ggccagcgcc tcgattcccg
      901 ctcgcaggcc tcgcgccacg ccgaacccga cggcgacgat ggtgccggcc accgccaaca
      961 gaacgatcgt cttcttgatc acttgacgtt ccctgccgtc ccgccaccgg cgtccccgct
     1021 gccctcggcg agcggatcga cgagcgtcac gcgcgcgccg gactccactc ccttcaccgc
     1081 gatctcgtcg gaattgcgcg cgaggacctc cacttcgaca gtccggtact catcgccatc
     1141 gaccaggtgg accgtcggtt tgccgttgcg ggtaaagagt gccttggcgg ggatgatcgt
     1201 cgcgcccggg atacgcctgg tgacgatgtc cagcgtcccg ttcatgccgg gcctgacgcg
     1261 cgagtcgctc ttgtcaccca gcgccgcata cgcgtgaaag ctccgcccgc gcgaatcgag
     1321 cgacatctcc gccatcggcg agatcccggt gagcttcgcc tgaatggaca cgtcgggaag
     1381 ggcatcgacg cgcacgatga cctcgtcgcc gatgcgcatc cggccgcggt cgatctcttc
     1441 cacggttgcg tcgatcagca gcgacgacag gtcgggaatc gtggcgaggt tcatgccgga
     1501 cgccaccggg tttccgacgc ggtatggctg ttcggcgccg ccgctcagtt gtgaggacag
     1561 cgagctgcgg ttggccgtgt agagcgcgaa cccggagatc ggcgccgaga tctccatttg
     1621 cgagatgcgc gactccacga tcctgaccca cccttcggcg gccgcgagct gtctctggag
     1681 cgaggcgatt ttcgattcac gcgatgcggc gcgctgcgcg acctccgcct cgagctgccg
     1741 cagcttctgt tcggcgacgc gcaggtcgat gcggccgcgc tcggcatcca ggcgcgccac
     1801 gaactcgctg tccttggtgt cgagctgcgc gatctccacg gcgagt
//
LOCUS       DBNGSH010000612         1577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2438477, whole genome shotgun sequence.
ACCESSION   DBNGSH010000612 DBNGSH010000000
VERSION     DBNGSH010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2438477"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="AC16CY_14150"
     CDS             complement(<1..410)
                     /locus_tag="AC16CY_14150"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR3354987.1"
                     /translation="MNTWRTFLHRLGYLPRKGRFDRQLDEELQFHVESRVDELEQQGY
                     PRPEARLRAVAEFGSRTRVAEESRAAWQFRWLEDVVADGSYALRAFRRRPTFALAAIA
                     CLALGIGANALIFSLVNAAFLRPLPYPDADRIAMV"
     gene            complement(425..757)
                     /locus_tag="AC16CY_14155"
     CDS             complement(425..757)
                     /locus_tag="AC16CY_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3354988.1"
                     /translation="MGKGSGELVQGTLPLLILRTLATGPNHGFAIARRIQQVSKDVLR
                     VEEGSLYPALHRLEVQGWIASEWRMTENNRTAKYYRLTRAGRRQLEREATRWNAIADA
                     IAAVLREA"
     gene            complement(847..1395)
                     /locus_tag="AC16CY_14160"
     CDS             complement(847..1395)
                     /locus_tag="AC16CY_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3354989.1"
                     /translation="MNNGIWSIGFMTVTARLRKLLKRLAASPMKKSEFLMAPMLSRLV
                     FLGPEVAVPLAFVAIAFGTPIRGSIVNIAAIAVLGAFAFGSIGLLVGSRARTFEAASG
                     LMNATTVPMWVLSGVFFSSSNFPDAVQPVIQALPLTALVDAMRGVILEGATLAGVWNE
                     IAILSAWTLVPFVIALRIFKWR"
     gene            complement(1392..>1577)
                     /locus_tag="AC16CY_14165"
     CDS             complement(1392..>1577)
                     /locus_tag="AC16CY_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354990.1"
                     /translation="PPAYRFDPAREESRVARLVVDEALKRAGGRPEPWTAREDRIAVP
                     GSRYVDWLVPPSSASAS"
BASE COUNT          284 a          537 c          522 g          234 t
ORIGIN      
        1 accatcgcga tgcgatcggc gtctggatac gggagcgggc gcaggaacgc cgcgttgacc
       61 aggctgaaga tcagggcatt cgcgccgatc ccgagtgcca ggcaggcgat ggccgccagg
      121 gcgaacgtcg ggcgacggcg aaacgcccgc agggcgtagc tgccgtccgc gaccacgtct
      181 tcgagccagc ggaactgcca ggcggcgcgg ctctcttccg ccacgcgcgt gcgcgaaccg
      241 aattcagcga cagcacgcag gcgggcctcg ggccgcggat agccttgctg ctcgagctcg
      301 tcgacacggc tttcgacgtg aaactggagt tcttcgtcga gctgccgatc gaaccgtcct
      361 ttccggggca ggtagccgag gcgatgcagg aacgtgcgcc acgtgttcat gagggacaag
      421 gctcctacgc ttcccgcagt acagcggcga tggcgtcggc gatggcgttc caccgagtcg
      481 cctcgcgttc caactgcctg cgaccggcgc gcgtcagccg gtagtatttc gctgtccggt
      541 tgttctccgt catgcgccac tccgacgcaa tccacccctg cacctcgagc cggtgcagag
      601 cgggatacag cgagccttct tcgacccgca ggacgtcctt cgacacctgc tggatgcggc
      661 gcgcgatggc aaacccatgg ttaggcccgg tcgccagggt acggaggatg aggaggggca
      721 acgtgccctg cacgagttcg ccggagccct ttcccataga tgcttgatgc gagacactat
      781 cgcgaggcac cgctcatgtc aagcatctag gggaacgtcg tcctcacgcc gcgaactccg
      841 tcgagctcac ctccacttga agatgcggag ggcgatgacg aaaggcacga gggtccaggc
      901 cgacagaatc gcgatctcgt tccagacgcc agccagcgtg gcgccctcga ggatgacgcc
      961 gcgcatcgca tccaccagcg ccgtgagcgg gagcgcctga atgaccggct gcaccgcatc
     1021 cgggaagttc gatgacgaga agaacacgcc gctcagcacc cacatcggca cggtcgtcgc
     1081 gttcatcagg cccgaggcgg cctcgaaggt ccgcgcgcgg ctgccgacga gcagcccgat
     1141 cgatccaaag gcgaacgcgc cgaggacggc gatggcggcg atgttgacga tggagccgcg
     1201 gatgggcgtc ccgaacgcga tcgcgacgaa cgccagcggc acggcaacct ccggaccgag
     1261 gaacaccagg cgcgacagca tcggcgccat caggaactcg ctcttcttca tcgggctggc
     1321 cgcgagccgc ttcagcagct tccgcagccg cgccgttacg gtcatgaacc cgatgctcca
     1381 gatgccgttg ttcatgatgc cgaggctgat gatggcggga ccagccagtc gacgtagcgc
     1441 gacccgggca ccgcgatcct gtcttcgcgc gccgtccacg gctccggccg gccaccggcc
     1501 cgcttcaacg cctcgtccac gacgaggcgc gcgacacggc tttcctcccg cgcgggatcg
     1561 aagcgatagg ccggcgg
//
LOCUS       DBNGSH010000613         1993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_231185, whole genome shotgun sequence.
ACCESSION   DBNGSH010000613 DBNGSH010000000
VERSION     DBNGSH010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_231185"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="AC16CY_14170"
     CDS             <1..668
                     /locus_tag="AC16CY_14170"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR3354991.1"
                     /translation="YAFSSILAALLRRATTGAGAVLDISMFDALAEWMGYAAYYTEYG
                     GTTLPRTGARHAAIAPYGPYTAGDGQVVYLGLQNEREWTRFCRDVLARPELESDPRFS
                     SAALRVDNYAALGAIITSTFAALTAEEVVRRLEAAQIANARMNTVAEFIAHPQLAARQ
                     KWRLVDSPAGALRALVPPFGFDDAEPRMDPIPALGEHTELILRELGIDEATIASWRAA
                     GIV"
     gene            complement(703..1491)
                     /locus_tag="AC16CY_14175"
     CDS             complement(703..1491)
                     /locus_tag="AC16CY_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3354992.1"
                     /translation="MRILDGKCALITGSVRGLGLAAAHRFAAAGCNVVLNGFDAERDV
                     SAIQKDIETGHGVRTLYHAGDLRRPADIESMVAAAQASFGSIDVVVNNAVVRHTAPVE
                     SFGTTEWDDGIAVNVSAAFHTIRLTVPGMKQRGWGRIINVSSIYGLRGAVNRVNYVTS
                     KTALLGLTRAVALEMVAHGVTCNAICPGTTETPVHEAAVAASMAADGISREDAERHVL
                     SGKQPTGRFISADSVAALMVFLCGPDGADITGAVLPVDGGWSGS"
     gene            1566..>1993
                     /locus_tag="AC16CY_14180"
     CDS             1566..>1993
                     /locus_tag="AC16CY_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354993.1"
                     /translation="MTRRTLVLVGGLALAAAVALPRSGALAQGQARPGGSVFLTPWGD
                     PDIQGLFTTDDELGVPFERPEQAGERDVVTDQEFADRQSQAARQAANDAEAFVAPSPG
                     RGGGGTGPPGHWLERGKPSRRTSIVIDPPNGRIPFLNDEAR"
BASE COUNT          320 a          714 c          653 g          306 t
ORIGIN      
        1 tgtacgcctt ctcgtcgatc ctcgccgcgc tcctgcgccg ggcgaccacg ggcgcgggcg
       61 ccgtgctcga catctcgatg ttcgacgccc tcgccgagtg gatggggtac gcggcgtact
      121 acaccgagta cggcggcacg acgctgcctc gcaccggggc ccgccacgca gcgatcgctc
      181 catacggtcc ctacaccgcc ggcgacggcc aggtggtcta cctcgggctg cagaacgaac
      241 gcgagtggac gaggttctgc cgtgacgtgc tcgcccgccc cgagctggaa tccgatccgc
      301 gcttcagctc cgccgccctt cgcgtcgaca attacgcggc gctcggcgcg atcatcacga
      361 gcacgtttgc ggcgctgacg gcggaagagg tggtccgccg gctcgaggcg gcgcagatcg
      421 ccaacgcgcg catgaacacg gttgccgagt tcatcgctca cccgcagctg gccgctcgtc
      481 agaaatggcg gctggtggat tcacccgccg gcgcgcttcg cgcgctcgtc ccgccattcg
      541 gtttcgacga cgccgaaccc aggatggacc cgattcccgc tctcggcgag cacacggagc
      601 tgattctccg cgaattgggc atcgacgagg ccacgatcgc ctcgtggcgc gcggcgggga
      661 tcgtttgaac agtcacttcg gatctcgaat cctggatgct cgctaactcc cggaccagcc
      721 tccgtcgacg ggaagaaccg ctccagtgat gtcggcgccg tcgggcccgc acagaaacac
      781 catcaacgca gccacgctgt cagccgaaat gaagcgcccc gtgggctgct ttcccgacag
      841 cacatgccgt tctgcgtcct cgcgcgagat cccgtcggca gccatcgacg cggcgacagc
      901 ggcttcgtgg accggcgtct ccgtggttcc cgggcagatc gcattgcacg tgacgccgtg
      961 ggcgaccatc tcgagcgcca ccgcgcgcgt caggccaagc agcgccgtct tgctggtgac
     1021 gtagttcacg cggttcaccg cgccgcgaag gccgtagatg gacgacacgt tgatgatccg
     1081 accccaccct cgctgcttca tgccgggaac cgtcagccgg atcgtgtgaa acgccgccga
     1141 cacgttcacc gcgatcccgt cgtcccattc cgtcgtcccg aagctctcca ccggcgcggt
     1201 gtgacggacg accgcgttgt tgacgacgac gtcgatcgat ccgaacgacg cctgcgcggc
     1261 cgcgaccatc gactcgatgt ccgccgggcg gcggagatcg ccggcgtggt agagcgttcg
     1321 cacgccgtgc cccgtttcga tgtccttctg gatcgccgag acgtcccgct ctgcgtcgaa
     1381 gccattcaga acgacgttgc agccagcggc ggcaaagcgg tgcgccgctg caagccccag
     1441 cccgcgcacc gacccggtga tgagggcaca cttaccgtca agaatgcgca tcagggatta
     1501 ccagcgaccg gccaccagct accggctaga ggctgttgta tcgtaaggca cgaaaggcga
     1561 agcacatgac cagacggaca ctcgtgctcg tgggtggact cgcgctggcg gcggccgtcg
     1621 cgctgccacg ctccggcgcc ctggcccagg gtcaggccag gccgggcggc agtgtgttcc
     1681 tcacgccgtg gggcgatccc gacattcagg gtctcttcac gaccgacgac gagctcgggg
     1741 tgcccttcga gcggccggag caggccggcg agcgcgacgt ggtgaccgat caggaattcg
     1801 ccgacaggca gtcgcaggcc gcgcggcagg cggccaacga tgccgaggcg ttcgtcgcgc
     1861 ccagcccggg ccgcggcggc ggcggaaccg ggccgcccgg ccactggctc gagcgcggga
     1921 agccttcgcg ccggacctcc atcgtgatcg atccgccgaa cggccgcatt cccttcctca
     1981 acgacgaggc acg
//
LOCUS       DBNGSH010000614         6582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2948146, whole genome shotgun sequence.
ACCESSION   DBNGSH010000614 DBNGSH010000000
VERSION     DBNGSH010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2948146"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="AC16CY_14185"
     CDS             complement(<1..962)
                     /locus_tag="AC16CY_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC domain-containing protein"
                     /protein_id="HIR3354994.1"
                     /translation="MTSETHSPTPEEPVLLRALHILRRRMAVAVVVFASVLAAAAAFA
                     VYLPDLFRARAVVLVERRLQEAFARADASDEVESRLHVIKQEVLSRARLTDLITRFDL
                     YPDLRAGGDMGAALEQVRRDIHIELTGPEQVSGRARTVAFNLTFTGSSRTAVADVTNA
                     IAAFYVAENQQMRSKDAARITEFLEAQMRDARAQLELDQTQVEAYTNRHAGELPQQVD
                     VNLATLERLNTQLRLNGDRQIRALDQRDRLFDVTSGSPEIPVPAGTSPEAARLAKLKG
                     ELAQLDGFPDRNPDVRRIKDEIAALEKAGGTGPAGSSVAGPARTA"
     gene            complement(974..1603)
                     /locus_tag="AC16CY_14190"
     CDS             complement(974..1603)
                     /locus_tag="AC16CY_14190"
                     /inference="COORDINATES: protein motif:HMM:NF046672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR3354995.1"
                     /translation="MMRPFVVTYACLCVSSVALAQVPPRSDARQPDPTAVTAARGRDA
                     RTVTDDREYKIGPEDVLDISVWKNPELSRTVPVRPDGKISLPLVNDIQAAGLTPSLLR
                     QELTQRLSEYVPSPEVAVIVREVNSATVAVVGAVRTPGRYGVRSPSTVLEIIALAQGF
                     TDFAARDRIVVLRRVGRIDTRIPFNYRNVADGSAQDNFFVQPGDIIVVP"
     gene            complement(1600..2778)
                     /locus_tag="AC16CY_14195"
     CDS             complement(1600..2778)
                     /locus_tag="AC16CY_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3354996.1"
                     /translation="MTRRIPVVVAVGLLAATNAGAQETTTTLAPAISVSTAHDDNVFS
                     TPQGVTDIVTTIQPSLESQLRSPTVNLQSLVSFDMQQSARHTLPTTLAARRHAMFDGR
                     VRTSPSVLLGLTGLYDRTETASDLSLNAGLLLPLQPAQILQTTPSVAFRVTPVLTITG
                     HYDWTHQALIGSPASDLHIADLGVARQSSSRTTWSAGYIERRFVDDVAVFRSHASVLG
                     WARELRAGTKLSLQAGPRITSSRVVTPEVRAAFVRQTPGTHVLVDYWRGDTMVLGIHG
                     PVEVQRGTTSLTWTAHRRLNLGTTLGVFRSAALDAPATTLYHAALVSTWRHEPYLITV
                     SYSTDLQRGSIALGGSAGEQVRRNVLLVRLTVAPRLSRAFRPPDDADQPARLATGVIP
                     "
     gene            complement(2775..3323)
                     /gene="nusG"
                     /locus_tag="AC16CY_14200"
     CDS             complement(2775..3323)
                     /gene="nusG"
                     /locus_tag="AC16CY_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR3354997.1"
                     /translation="MSEEAWYAVWLRSHCEHLVARQLSAKGFHTFLPELPTWSPGLAL
                     NCSGRTPMFPGYLFVRDAMNKTRYLDLLKARGIVRVLEAGWSRLTPVPDEDIDAIQRI
                     VLAAVPVYPHSHLRSGDRVRVSEGPLTGLEGIFVQDRASKGRLVVSVNMLGRSVAVDV
                     HRADVEACAPAASGMRPIGGPR"
     gene            3686..5791
                     /locus_tag="AC16CY_14205"
     CDS             3686..5791
                     /locus_tag="AC16CY_14205"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3354998.1"
                     /translation="MDNARRSRHALRVVVLEDPGALRQWATAWEDLAAHAAEPNPFYE
                     PWMLLPAIQFLSRPAQLLFVLVTAESPVSGLSDVLHGLFPIERSSRYRGLPLKCLSIW
                     KHRYCYLCTPLIRKGSEEDALAALFDWLATDPHGATLVEFPQVTDDEPVFRALVTLTG
                     ERRRPFFLSKRETRAMLRRRADAEAYIEEALPGKSRKEYRRLEHRLSELGRLEYGEPG
                     PAEIDAWIDAFLELEARGWKGRLGTAMASTEAGRRFFTAVARAGPDRVMLLGLHAGGT
                     PVALKCNLLAGDGAFAFKIAFDEALGRFSPGVLLELENIRRFHHRAPRWMDSCAVPDH
                     FMANRLWLDRRTVSTLLVSTGGAAGDLLVASLRPLRSAFLMMRGSRGTKAGHATHADD
                     GRPSTGTRHRRVSASGQGASLLDVAPEALRASFGRTPFRVRHHLGESGLFQLPRLLEL
                     ARHLPESSVEYNAGNVPITLDPSETPRTGLSVEETLRRIESCRSWMVLKNVEQDPEYR
                     DLLETCLEEVRRLSHAVSPGMSRKEGFIFISSPGSVTPYHMDPEENFLLQIRGRKTIH
                     VFDARDRSIVSDEQIERFLTGANRNLVFRDEYQPRARIFPLGPGKGVHVPVTAPHWVQ
                     NGEEVSISFSLTFQTRSSIRLTHAHRMNAAMRRWGLEPRPVGDSLWRDVVKQFAYRAT
                     NRVSRMLPELPAPRRVSQR"
     gene            5797..6435
                     /locus_tag="AC16CY_14210"
     CDS             5797..6435
                     /locus_tag="AC16CY_14210"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3354999.1"
                     /translation="MSNASPTRHAVHASPVHALPRLPYGFDALEPHIDSQTVQIHYRK
                     HHQAYIDSLSAALEDYPELQSRTAEELCRDILSIPEGIRTVVRQSAGGHWNHCRLWAW
                     MTPNSGGAPIGRSSEAIAASFGDVTAFKEQFSAAAIGVFGSGWAWLVKDGISLSITTT
                     PNQDNPLMEGITPILGLDLWEHAYYLKYQNRRADYVRAWWSVVNWTEVNKAY"
BASE COUNT         1104 a         2184 c         2177 g         1117 t
ORIGIN      
        1 gccgtgcgcg ccggtccggc cacagaggat ccggccgggc ccgtgccgcc ggccttctcc
       61 agcgcggcga tctcgtcttt gattcgcctg acatccggat tccggtctgg gaagccgtcc
      121 agctgcgcca gctcaccctt cagtttcgcc agccgggcgg cctcgggaga cgtgccggcg
      181 ggcacgggga tctctggcga gccactggtc acatcgaaca gcctgtcccg ctggtcgagc
      241 gcccgaatct gccggtcgcc attgagccgc agctgcgtgt tcaaccgctc gagcgttgcc
      301 aggttcacgt cgacctgctg cggcagctcc ccggcgtgcc ggttggtgta cgcctcgacc
      361 tgcgtctggt ccagctcgag ttgcgccctg gcgtcccgca tctgtgcctc gaggaactcc
      421 gtgatgcgcg ctgcgtcctt ggaccgcatc tgctggttct cggcgacata gaacgcggcg
      481 atggcgttgg tcacgtcagc gaccgccgtg cgcgagctcc cggtgaacgt caggttgaag
      541 gcgaccgttc tggctcggcc actcacctgc tcggggccag tcagctcgat atggatgtct
      601 cgtcgcacct gctcgagcgc agcgcccatg tcgccaccgg ctcggagatc gggatacagg
      661 tcgaagcggg tgatcagatc cgtcaggcgg gcgcggctga ggacctcctg cttgatcacg
      721 tgcaggcggc tttcgacctc gtcgctcgcg tccgccctgg cgaacgcctc ctggagccgc
      781 cgctcgacga gcaccaccgc tctcgcccga aacaggtccg gcaggtacac ggcaaacgct
      841 gcggcggccg ccagaaccga cgcgaagacc acgacggcca cagccatccg ccgccggagg
      901 atgtgcagtg cgcgaagcag aaccggttct tcaggcgtcg gcgaatgcgt ctcggatgtc
      961 atggcaatgt gtctcaaggg acgacgatga tgtcgccggg ctgcacgaag aagttgtcct
     1021 gcgctgaacc gtcggcgaca ttgcgatagt tgaagggaat gcgtgtgtcg atgcggccga
     1081 cccggcgcag gacgacgatg cgatcgcggg cagcgaagtc ggtgaatccc tgggcgagcg
     1141 cgatgatctc gagcaccgtc gacggactcc gcacgccgta gcgcccgggg gtacggaccg
     1201 cgccgacgac cgcgacggtg gcgctgttca cctcgcgaac gatcacggcg acttcggggg
     1261 agggcacata ctccgacagc cgctgtgtca gctcctgacg caggaggctc ggagtcaaac
     1321 cagccgcctg aatgtcgttg accagcggca acgagatctt cccgtctggg cggacgggca
     1381 ccgtccggct cagttcgggg ttcttccaca ccgagatgtc cagcacgtcc tcgggcccga
     1441 tcttgtactc ccggtcgtcg gtgacagtcc gcgcgtcccg gccccgtgcg gccgtcacgg
     1501 cggttgggtc gggctggcgt gcgtcgcttc gcggcggcac ctgggccagc gccacggagg
     1561 agacgcacag acacgcatac gtgacgacga agggtcgcat catgggatga ctccagtggc
     1621 cagtctcgcg ggctgatcgg catcgtcggg cggcctgaac gctcgactga ggcgcggtgc
     1681 gacggtcagg cggacgagca ggacgttgcg gcgcacctgt tcgcctgcgg atccgccgag
     1741 ggcgatgctg ccgcgctgaa gatcggtgct gtagctgacg gtaatgaggt aaggctcgtg
     1801 gcgccacgtg ctgaccagcg ccgcgtgata gagcgtcgtg gcgggcgcgt cgagagcggc
     1861 gctgcggaac accccgagag tggtcccgag gttgaggcgc cggtgcgcgg tccaggtcag
     1921 gctggtcgtc ccccgctgca cctccactgg accgtgaatc ccgaggacca tcgtgtcccc
     1981 gcgccagtaa tccacgagga cgtgcgttcc gggagtctga cggacgaagg ccgctcgcac
     2041 ctccggggtg acgacgcggg acgatgtgat gcgtggcccg gcctgaagtg agagcttcgt
     2101 cccggcccgc aactcgcgcg cccatccgag cacactcgcg tgcgaccgga agaccgcgac
     2161 gtcatcgacg aaccgccgtt cgatataccc ggcgctccac gtcgtgcgcg atgacgactg
     2221 gcgcgcgaca ccaaggtcgg cgatatggag gtcgctcgct ggcgagccga tcagggcctg
     2281 gtgcgtccag tcatagtgac cggtgattgt caacacgggt gtcacgcgaa aggcgaccga
     2341 tggcgtggtc tggaggatct gcgcgggctg cagtgggagg aggaggcccg cattgagcga
     2401 gaggtcgctg gccgtctccg tgcggtcgta caggccggtc agacccagga gcaccgatgg
     2461 cgaggtccgc acgcgaccgt cgaacatggc gtgccgacgc gcggccaatg tcgtcggcaa
     2521 cgtgtgccgg gcagactgtt gcatgtcgaa cgagacgagg ctctgaaggt tcaccgtcgg
     2581 cgatcggagc tgactttcga gagacggttg aatggtggtg acgatgtcgg tgactccctg
     2641 cggcgtcgag aagacgttgt cgtcgtgcgc cgtgctcacc gagatcgctg gcgccagcgt
     2701 ggtggtcgtc tcctgcgccc cggcattggt ggcagccaac aacccgaccg ccacgacgac
     2761 gggaatccgt cgcgtcatcg cggcccaccg atcggtcgca tgcctgacgc cgctggcgcg
     2821 cacgcctcga cgtctgcgcg gtgcacgtcg accgcgacgc tgcgcccgag catgttcacc
     2881 gagacgacga gccgtccttt ggacgcacgg tcctgcacga agatgccttc gagcccggtc
     2941 agtggaccct cggacacgcg aacgcggtca ccggatcgaa ggtgggagtg agggtagaca
     3001 ggcaccgccg ccagcacgat ccgctgaatg gcgtcgatgt cctcgtctgg taccggcgtc
     3061 aaccgtgacc accccgcttc gagcacgcgc acgatgccgc gcgccttcag caggtcgagg
     3121 tagcgtgtct tgttcatggc gtcgcgcacg aacaggtatc caggaaacat gggcgtacgg
     3181 ccggagcagt tcagcgcgag tcccggcgac cacgtcggca gctcgggcag gaaggtgtgg
     3241 aatccctttg ccgacagttg ccgcgcgacc aggtgttcgc aatgactgcg cagccacacg
     3301 gcgtaccacg cctcttctga cacagcgcca ctgccggcac tgtggccgtc tccctgacgt
     3361 tgtcggtccg atccgtccac gaccagcaag gcttcgcccc tttcggtttg taatccttac
     3421 ggcaaaccgt aaactttgcc gcctgtctcc gcgttgaaat cgtctccgcg ccgccgacag
     3481 gcccgttcac cgcctgaagc gccgactgcg atcgggctca cgtggtggac gaccatccgg
     3541 tgtgcatgaa tcgtgctgac aacgaaagcg agcaaccgca atcgccgcga ccgtcgcggc
     3601 gcactccttg cacccgccct cgacgttgag tgtgcgtcgg taagagttgc ctcgcccgcg
     3661 ctgcgggtga cgaccagtga acaagatgga caatgcccga cgcagtcgcc acgcgctgcg
     3721 tgtggtcgtg ttggaggatc ctggcgcgct ccgccaatgg gcgaccgcgt gggaggacct
     3781 ggcggctcac gccgccgaac ccaacccgtt ctacgagccg tggatgttgc ttccggcaat
     3841 ccagttcctc agcaggccgg cgcagcttct gttcgtcctc gtcacggcgg agtcacccgt
     3901 cagcggcctg tcggatgtgc tccatggcct cttccctatc gaacgctctt cccgctaccg
     3961 gggtctgccg ctgaagtgct tgtccatctg gaagcacagg tactgctatc tctgcacacc
     4021 gctgatccgg aagggctcgg aggaggacgc gttggcggcc ctcttcgact ggctcgcgac
     4081 ggatcctcac ggggccacgc tcgtcgagtt cccgcaggtg acagacgacg agccagtctt
     4141 ccgggctctg gtcacactga cgggagagag aaggaggcct ttcttcctct ccaagcgtga
     4201 gacacgtgcc atgctgcgcc ggcgagcgga tgccgaggcc tacatcgagg aggcgctgcc
     4261 gggaaagagc cggaaggagt atcgccgact cgagcatcgc ctctcggagc tgggtcggct
     4321 cgagtacggc gagcccggac ccgcggagat tgacgcctgg atcgacgcct ttctggagct
     4381 cgaagccagg ggatggaagg gccgcctcgg gacagccatg gcgtcaaccg aggcggggcg
     4441 acggttcttt accgcggtcg ctcgagccgg ccccgatcga gtcatgctcc tcggcctcca
     4501 cgcgggtggt acgccggtcg ccctcaagtg caacctcctg gccggcgacg gagccttcgc
     4561 gttcaagatc gccttcgacg aggcgctagg acgcttctcc cccggtgtgc tgctcgagct
     4621 cgagaacatc cgcaggttcc accacagggc accgcgctgg atggattcgt gcgccgtccc
     4681 cgaccacttc atggccaaca ggctctggct ggatcgacga acggtgagca cgctcctcgt
     4741 cagcaccgga ggtgccgccg gggacctgct cgtcgcgtct ctacggccgt tgcggagcgc
     4801 attcctcatg atgcgtggtt cgcgcggcac gaaggccgga catgccacgc acgcggacga
     4861 cggccgcccg tccaccggga cacgtcaccg acgtgtctcg gcgagcggcc agggcgccag
     4921 cctcctggac gtggccccgg aggcgttgcg cgcgagcttc ggccggacgc catttcgcgt
     4981 acgccatcac cttggggagt cgggactgtt ccagctccct cggctgctgg agcttgcgag
     5041 gcacctgccg gaatcgagcg tcgagtacaa cgcggggaac gtgccgatca ctctggaccc
     5101 gtccgagacg ccacgaacag gactctccgt ggaggagacc ctccggcgga tcgagagctg
     5161 ccggtcctgg atggtgctca agaacgtcga gcaggatccg gagtaccgcg atctgctcga
     5221 gacctgtctg gaagaagtcc gtcggctctc gcacgcggtc agccctggca tgagccgcaa
     5281 ggagggcttc atcttcatct cctcgccggg atcggtgacg ccgtatcaca tggaccccga
     5341 agagaacttc ctgctccaga tccgcggacg caagaccatc cacgtcttcg acgcgcggga
     5401 ccgctcgatc gtctccgacg agcagatcga gcgctttctc accggggcca accgcaacct
     5461 cgtcttccgg gacgagtatc agcccagggc tcgcatcttt cctctcggtc ctggcaaggg
     5521 cgtacacgtg ccggtgaccg cgccgcactg ggtccagaac ggcgaggagg tctccatctc
     5581 gttcagcctc acgttccaga cccgatcctc gatccggttg acacacgcgc accgcatgaa
     5641 cgccgcgatg cgccgatggg gtctcgagcc caggcccgtc ggcgattccc tctggcgcga
     5701 cgtggtcaag cagttcgcct atcgcgccac gaacagggtg tccaggatgt tgccggaact
     5761 gcccgcgccg cgtcgtgtgt cacaacggtg aacaagatga gcaacgccag ccccacgcgc
     5821 cacgccgtcc acgccagccc ggtgcacgca ctgccccgac tgccgtacgg cttcgatgct
     5881 ctcgagccgc acattgattc gcagacggta cagattcact accgaaaaca tcatcaggcg
     5941 tacatcgaca gcttgagcgc ggcgctcgag gactaccccg agctgcagtc gagaacggcc
     6001 gaggagctct gtcgcgacat cctcagcatc cccgaaggga ttcgcacggt ggtccgacaa
     6061 tccgccggcg ggcactggaa ccactgccga ctgtgggcgt ggatgacgcc gaactccggc
     6121 ggggccccga ttggcaggtc ctccgaggcg attgccgcct cgttcggtga cgtcaccgcc
     6181 ttcaaggaac agttctcggc ggccgcgatc ggcgtgttcg gcagcggctg ggcgtggctc
     6241 gtcaaggatg gcatcagcct ctcgattacg accacaccaa atcaggacaa cccgttgatg
     6301 gagggaataa cgccgattct gggcctcgac ctgtgggaac acgcctatta cctgaagtac
     6361 cagaaccgac gggccgacta cgtcagggcc tggtggagtg tcgtgaactg gaccgaggtc
     6421 aacaaggcgt actgaagcgc tccgccgccc gcgagccgtg ccgtcccgat gcgtgtcttt
     6481 gtgaacacgt gtctttttgg actgcgctca cattctgcat cggtcacatt tacctcgcac
     6541 tgaatctgga gtctgaaacc cgagcgttga atcggcgatc gg
//
LOCUS       DBNGSH010000615         1587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4647268, whole genome shotgun sequence.
ACCESSION   DBNGSH010000615 DBNGSH010000000
VERSION     DBNGSH010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4647268"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1391
                     /gene="argG"
                     /locus_tag="AC16CY_14215"
     CDS             51..1391
                     /gene="argG"
                     /locus_tag="AC16CY_14215"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR3355000.1"
                     /translation="MATILQRLPVGERVGIAFSGGLDTSAALHWMRAKGAIPYAYTAN
                     LGQPDERDYDEIPRKALQYGAEKSRLVDCRAQLVAEGLAALQCGAFHISTAGVPYFNT
                     TPIGRAVTGTMLVLAMKSDDVHIWGDGSTYKGNDIERFYRYGLLANPSLRVYKPWLDQ
                     AFIDELGGRKEMSQYMERAGLAYRMSTEKAYSTDSNMLGATHEAKDLEYLHRGIAIVE
                     PIMGVAFWREDLPIRPEQVTIGFEEGLPVSLNGQRFADRVTLVDEANRIGGRHGIGMS
                     DQIENRIIEAKSRGIYEAPGMALLFAAYERLLTGIHNEDTIELYRDNGRRLGRLLYQG
                     RWFDPQSMMLRAAQGWVARAVTGEVVLELRRGNDYSILDTRSANLTYKPERLTMERGE
                     SAFTPQDRIGQLTMRNLDIADTRDKLLAYGRSGLLGDGASLTHLLEDETKSSKR"
     gene            complement(1394..>1587)
                     /locus_tag="AC16CY_14220"
     CDS             complement(1394..>1587)
                     /locus_tag="AC16CY_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355001.1"
                     /translation="PIGPFDVSGAYDEASDRAQQLNELMERAYEDAYRQFIEPVVGAS
                     GEQLAHATAGAVKEEFRLR"
BASE COUNT          292 a          528 c          527 g          240 t
ORIGIN      
        1 ttcgcgcgaa ggcgggcact ttcaggttcg tgttctatca tggagaccgc atggccacga
       61 tcctgcagcg ccttcccgtc ggtgaacggg taggcatcgc attctccggc ggcctcgaca
      121 cgagcgcggc gctccactgg atgcgcgcga agggcgcgat cccctacgcc tacaccgcga
      181 acctgggcca gccggacgaa cgggactacg acgagattcc gcggaaggcg ctccagtacg
      241 gcgcggagaa gtcgcgtctc gtggactgtc gcgcgcagct cgtcgccgag gggctggcgg
      301 cgttgcagtg cggcgccttc cacatctcca cggccggtgt cccgtacttc aacacgacgc
      361 cgatcggacg agcggtgacc ggcacgatgc tcgtgctcgc catgaagagc gacgacgtgc
      421 acatctgggg cgacgggagc acgtacaaag ggaacgacat cgaacgattc taccgctacg
      481 gcctgctcgc caacccgtcg ctgcgtgtct acaagccgtg gctcgaccag gccttcatcg
      541 acgagctcgg cggacgcaaa gagatgtcgc agtacatgga gcgcgccggc ctggcgtacc
      601 ggatgagcac cgagaaggcc tactccacag actcgaacat gctgggcgcc acgcacgagg
      661 cgaaggatct cgagtacctc caccgcggca tcgccatcgt cgagccgatc atgggcgtcg
      721 cgttctggcg ggaggatctg ccgatcaggc cagaacaggt gacgatcggc ttcgaagaag
      781 gcctgccggt gtcgctcaac ggccagcgct tcgccgatcg ggtgacgctg gttgacgaag
      841 ccaaccggat cggcggccgc cacggcatcg gcatgagcga ccagatcgag aaccgcatca
      901 tcgaggccaa gagccgcggc atctacgaag ccccgggcat ggcgctgctg tttgctgcct
      961 acgaacggct gctgaccggc atccacaacg aggacacgat cgagctgtac cgggacaacg
     1021 gccgccgcct cgggcggctg ctctaccagg gccgctggtt cgatccgcag tcgatgatgc
     1081 tgcgcgcggc gcagggatgg gtcgcccgcg cggtcacggg cgaggtcgtc ctggagctcc
     1141 gccggggaaa cgactactcg atcctcgaca cgcgaagcgc gaacctgacg tacaagcccg
     1201 agcgcctcac gatggagcgg ggagaatcgg ccttcacgcc acaggatcgc atcgggcagt
     1261 tgacgatgcg gaacctggat atcgccgaca cgcgcgacaa gcttctcgcc tacggccgtt
     1321 ccggcctcct gggggacggc gcttcgctga cgcacctgct cgaagacgag accaagtcaa
     1381 gcaagcgctg aagctaccga agccggaact cctctttgac ggcgcctgcc gtggcgtgcg
     1441 cgagctgctc gccgctcgct ccgacgaccg gctcgatgaa ctgccggtac gcgtcctcgt
     1501 aggcgcgctc catcagctcg ttcagctgct gcgcgcggtc ggaggcctcg tcgtacgcac
     1561 ccgagacgtc gaaggggccg atcgggt
//
LOCUS       DBNGSH010000616         3383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4647342, whole genome shotgun sequence.
ACCESSION   DBNGSH010000616 DBNGSH010000000
VERSION     DBNGSH010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4647342"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="AC16CY_14225"
     CDS             complement(<1..1178)
                     /locus_tag="AC16CY_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015207291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3355002.1"
                     /translation="MIGQETLMNFVGGRWEAPGATQTIPVKNPATGDLLADVPLSSAA
                     DVGKAVQSASSALVAWRRTPPGDRIQPLFRLKALLDAHFSEIARLVTQECGKTLAESE
                     GELRRGIENVEVATGIPSLMMGSNLEDIASGIDELMIRQPVGVVGVVTPFNFPGMIPL
                     WFLPYAVACGNTLVLKPSEKTPLTMARVLQLVEQAGFPAGVVNLVHGGKDAVDALLDH
                     PDVRAISFVGSTPVARYVYSRAAANGKRVQCQGGAKNPIVVLPDADMDMTTRIVADSA
                     FGCAGQRCLASSVVITVAESARTFTPQIGDAAASRKIGYGLDKGVEMGPVISPESRAR
                     IEGLIAQGVAGGARPLVDGRNAKVEGYSRGNFLRPTVLTDVDPSSELARTEVFGPVLS
                     V"
     gene            complement(1350..2447)
                     /locus_tag="AC16CY_14230"
     CDS             complement(1350..2447)
                     /locus_tag="AC16CY_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3355003.1"
                     /translation="MRAAILEKAPGGMHVEEIPMPEPGAGEVLVKVSACGVCHTDLHV
                     MKAEVAFPTPAVMGHEISGTVAALGPGVNGPAVGTKVVSAFIMPCGFCTPCGAGRDDL
                     CDSFFAMNRLKGTLYDGTSRLKRRNGSSIAMYSMAGLAEYSVVPATDVFPLLDGLPLE
                     ESSVLGCAVFTAYGAVRHGADLRGGERIAVVAVGGVGINVIQIARAFGASQIIAVDVK
                     DDKLQAATRLGATDVVNASAADATARVRELTGGRGVDVAFEVLGLPQTFTQAFEMIRD
                     GGRMVAIGIAPGKTTAPVEITRLVRRELRIVGSYGARTRSDMPEIIRLAAQGIFRPET
                     MVTQRFSLNDADAAYQALARGEIVGRAIVVP"
     gene            complement(2585..>3383)
                     /locus_tag="AC16CY_14235"
     CDS             complement(2585..>3383)
                     /locus_tag="AC16CY_14235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3355004.1"
                     /translation="AGLVLSTAVFTLFSRMPEAQFLAWGWRVPFLLSALLVVVGLIIR
                     VRILETPSFSRLKEAKREARQPIVEVIRTYPKQVLLAMGARLAENGCFYIYSVFVLTY
                     ATQHVKMNQQVVLMAMLVGAGLELLAIPFFGFLSDRVGRRPVYLFGAAVTGLFAYPLF
                     LLLDTGSPLLVGLALVIAFLFSHAPMYGPQAAFLAELFGTRVRYSGASLGAQLAGVIA
                     GAPAPFVATLLLANYGNVALALYMVAMSVVTIIAVLVASETHREDIN"
BASE COUNT          600 a         1155 c         1082 g          546 t
ORIGIN      
        1 acgctcagca ccggcccgaa gacctcggtg cgcgcgagct cgctcgaggg atcgacatcg
       61 gtgagcacgg tgggccgcag gaagttgcca cggctgtagc cttccacttt cgcgttgcgg
      121 ccgtccacga gcggccgcgc acctccggcc acgccctgag cgatgaggcc ttcgattcgc
      181 gcgcggctct cgggggagat gaccgggccc atctcgacgc ccttgtccag gccgtacccg
      241 attttccgcg aggctgccgc atcgccaatc tgcggcgtga acgtcctggc ggattccgcc
      301 accgtgatga cgaccgatga ggccaggcag cgctggcccg cgcagccgaa cgcggaatcg
      361 gcgacgatgc gcgtcgtcat gtccatgtcg gcgtccggca gcacgacgat ggggttcttg
      421 gctccaccct ggcactgcac gcgcttgccg ttggccgcgg cccggctgta gacgtacctc
      481 gccacgggcg tcgagcccac gaaggaaatc gccctcacgt cggggtgatc gagcagcgcg
      541 tccacggcgt ccttgccgcc gtgcaccagg ttgacgacgc ccgcggggaa gccggcctgc
      601 tcgacgagct gaaggacgcg cgccatcgtc agcggtgtct tctccgacgg cttgagcacg
      661 agcgtgttgc cgcacgccac ggcgtacggc aggaaccaca acgggatcat cccggggaaa
      721 ttgaacggcg taaccacgcc gacgaccccc accggctgcc ggatcatcag ctcgtcgatg
      781 ccggatgcga tgtcttcgag gttgctcccc atcatcagcg acgggattcc ggtcgcgact
      841 tccacgttct cgatgccacg cctgagttct ccttcggact cggcgagcgt cttgccgcat
      901 tcctgcgtca ccaggcgcgc gatctcgctg aagtgtgcgt cgagcagcgc cttcaatctg
      961 aagagcggct ggatcctatc gcccggaggc gtgcgccgcc acgccacgag cgcgctcgag
     1021 gcggactgta cggccttgcc gacgtcggcg gccgatgaca gcggcacgtc ggccagcagg
     1081 tcgccggtcg cgggattctt caccggtatc gtctgcgtgg cgcccggagc ttcccagcgt
     1141 ccgccaacga agttcatcaa cgtttcttgc ccgatcattg ctgctccttc ggatttcttc
     1201 cgctcgtcac ctgatcacgc acgttaacca cagagacaca gaggacacag agatacttct
     1261 atcaaaaaga ggtacttcta tcagaaaaga tgactctgtg ttctctgcgc ctctgtggtt
     1321 gacgtacgtc tctgtgtttc cgaagtcgtt tacggaacga ctatcgcccg gccgacaatc
     1381 tcgcctcgcg ccagtgcctg gtacgctgca tccgcgtcgt tcagcgagaa ccgctgcgtc
     1441 accatcgtct cgggacgaaa gatcccctga gcggcgagcc tgatgatctc gggcatgtcg
     1501 ctccgggttc tcgcgccgta cgagccgacg atccgcagct cgcgccggac gaggcgggtg
     1561 atctccaccg gcgcggtagt cttgcccggc gcgatgccga tcgccaccat gcggccgccg
     1621 tcgcggatca tctcgaacgc ctgcgtaaac gtctgcggca ggcccaggac ctcgaacgcg
     1681 acgtctacgc cgcgtccccc ggtcagctcg cggacccgcg ccgtcgcgtc ggcggcgctc
     1741 gcgttgacga cgtcggtcgc gccgagccgt gttgccgcct ggagcttgtc gtctttcacg
     1801 tcgaccgcga tgatttgcga cgccccgaac gcacgcgcga tctggatcac gttgatcccg
     1861 actccgccga cggcgaccac ggcaatccgc tcgccgccgc gcaggtccgc gccgtgacga
     1921 accgccccgt acgccgtgaa gacggcgcac ccaaggaccg aggactcctc gagcggcaga
     1981 ccgtcaagca gcggaaagac gtcggtcgcc ggcacgaccg agtactcggc gagacccgcc
     2041 atcgagtaca tcgcgatcga ggacccgttg cgacgcttga ggcgggacgt gccgtcgtag
     2101 agcgtgccct tcagccggtt catcgcgaag aagctgtcgc acaggtcatc tcggccggcg
     2161 ccgcacggag tacagaagcc gcacggcatg atgaaggccg acacgacctt cgtgccgacg
     2221 gccgggccgt tgacccccgg gccaagcgcc gccacggttc ccgagatttc atggcccatg
     2281 acggcgggcg ttggaaaagc gacctcggct ttcatcacgt gcaggtcggt gtggcacacg
     2341 ccgcacgcgc tgaccttcac caggacctcg ccggcgcccg gctcgggcat cgggatctcc
     2401 tcgacgtgca tcccgccggg cgccttctcg agaattgccg ctctcatcgt ctgcatgtcg
     2461 tctccgaacc gagagcctag tggaacgtat ctcctgaatt actagcggcc gctccgttgt
     2521 cgcggccgcg aaagtgcggt cgaaaggctg tcgtcggcgc tacgggttca gttgatacag
     2581 tccactagtt tatgtcttcc cggtgcgtct ccgacgccac gagcacggca atgatggtga
     2641 cgacggacat cgcgaccatg tacagagcca gcgcaacgtt gccgtaattg gcgaggagaa
     2701 gcgtcgcgac aaacggcgca ggcgcgcccg caatcactcc ggcgagctgc gcgccgagcg
     2761 aggcgccgct gtagcggacg cgcgtcccga acagctctgc caggaacgcg gcctgcggcc
     2821 cgtacatcgg cgcgtgggag aacaggaacg cgatgacgag cgcgagcccc acgagcagcg
     2881 gcgaccccgt gtcgagcagc aggaacagcg ggtaggcaaa gagacccgtc acggcggcgc
     2941 cgaacaggta gacgggacgg cgcccgaccc tgtcggagag aaaaccgaag aacgggatcg
     3001 ccagcagctc gaggccggcg ccgacgagca tcgccatcag caccacctgc tgattcatct
     3061 tcacgtgctg cgtggcgtag gtcaggacga agacgctgta gatatagaag cacccgtttt
     3121 ccgcaaggcg cgcacccatc gccagcagga cctgcttcgg ataggtgcga atcacttcga
     3181 cgatcggctg ccgggcctca cgtttcgctt ccttgagcct cgagaaggag ggcgtctcca
     3241 gaatccgcac ccggatgatc aggccgacga cgacaaggag ggcgctcagg agaaacggca
     3301 cgcgccagcc ccaggccagg aactgcgcct ccggcatccg cgagaagagc gtgaagacgg
     3361 cggtggagag caccaggccg gcc
//
LOCUS       DBNGSH010000617         4303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2948507, whole genome shotgun sequence.
ACCESSION   DBNGSH010000617 DBNGSH010000000
VERSION     DBNGSH010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2948507"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="AC16CY_14240"
     CDS             complement(<1..223)
                     /locus_tag="AC16CY_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355005.1"
                     /translation="MRPSFNHVTNRTIGYLAAFLLLSCPHTASAITYMSIETVPNGDV
                     VGEDEVVTIRSIGYANLERWSQRLLDECRV"
     gene            325..2154
                     /locus_tag="AC16CY_14245"
     CDS             325..2154
                     /locus_tag="AC16CY_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3355006.1"
                     /translation="MRTSFNGSRSDPRAIAYARATIDLASRDVRIEDVPCRNLEDVLG
                     GFGRSFQILAERTITHAFTPDNPLIVNTGVLTASNVMTGLRTYFSAYSPLKVSNKGLP
                     AAIWSAGSCKFGPKLKWTGLDEVILEGRADVPVYLVIRHGEQGPRIAFEPAGDLLGLT
                     CHEKIMWLRDRYADAHFAAIGPAGEHYEDCYMASVALSTENQLRSGDDKCRWAGRGGM
                     GAIMGYKRVLAIVAQAPDRIAKLKPEIRDINKVASTGPGSRKFREKEKGGLGGTWSNY
                     EPLEKFHFVPQNNFRPTGDGKPELMFRDHVQPQFVVKAESCFRCGINCHKNVYEKNAD
                     GTRGAFLAKFDYEPVNLLSTNIGIHDPRKAATLISLVDRLGMDNISLGTTISYVMDYN
                     DRHPDAPILNGAKFGDFDKARELIEGAANGQFPEVGRGVKRLSEQLGETGYAMHCKGL
                     EFPAYQPDTNPGYPWAIAGGHMSMATYMALALEGNTSLDYWATLITERGLYFVRDDLL
                     GLCKFAGVTGDHAVEGLKHEIGFEVTPAELQAAVRRAFVRALWLERKQGYDRSDYTLP
                     SEVFDRPNPNLGVPPFVTREFFSQLSDRVWAVFDREIDEIAKT"
     gene            2156..2431
                     /locus_tag="AC16CY_14250"
     CDS             2156..2431
                     /locus_tag="AC16CY_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3355007.1"
                     /translation="MPRIRLTNLFAERIGGVNAVDVPATTVESALRALTDRHPSLTPL
                     VWAKDGALNPVMVMFLNDRQLAAAEIGTPVGPGDEIDLVPAIEGGCS"
     gene            complement(2426..2896)
                     /locus_tag="AC16CY_14255"
     CDS             complement(2426..2896)
                     /locus_tag="AC16CY_14255"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3355008.1"
                     /translation="MAKVKVSTEVKAPVDRVFELFTDIEHAAEHVSGIKKIEVLSTGP
                     FNLGYRWTETREVMGRLDDATMEISSFELNRTYTVTHYKGGVLGSGVRIDAIFTFEPV
                     PAGTKVSIEFELHPEGLPPAVLLPLEWAIGGKVRHVLSDDLADLKKSIETITGS"
     gene            3346..3786
                     /locus_tag="AC16CY_14260"
     CDS             3346..3786
                     /locus_tag="AC16CY_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355009.1"
                     /translation="MFSPSSLLLVHLVGLAIGLGAATTKLVLLFQCRADGAFVPTYLA
                     VVRPITRQIILGLVLLLLSGIGFMAIGHSFTPRLVTKLVLVSAIFVLGPVIDNAVEPR
                     FRVLAPRPGTEASPEFVRVRTQYLALELLATGLFYAVVIIWVVF"
     gene            3900..>4303
                     /locus_tag="AC16CY_14265"
     CDS             3900..>4303
                     /locus_tag="AC16CY_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3355010.1"
                     /translation="MKPSTSSKSNAKSILRFHHVLVPTDLTERTEKALELADKLTVSD
                     TSRITLMHVIETIEGMQFDELKPFYDRLERKARTTMTRFARRVPSMRVKNVLIYGRRA
                     EEIVKFAAANDVDLIVLASHRVAPSTVNRDWGT"
BASE COUNT          803 a         1366 c         1354 g          780 t
ORIGIN      
        1 ccacgcgaca ctcgtcgagc agccgctgac tccagcgctc gaggttggcg tagccaatgc
       61 tgcgaatcgt gacgacctcg tcctcaccga cgacatctcc gttcggaacc gtctcgatcg
      121 acatgtaggt gatggccgac gctgtgtgag ggcacgacag caggaggaag gcggccaggt
      181 aaccaatcgt ccggttcgtc acgtgattga atgacggccg catgacgatg ctccagtcga
      241 ggggaagggg gggtatctta cctcagacgt gtggtgtcga ccagtgcctg gagacacgcg
      301 acgtcacagt gataaattgt cgcgatgcgc acctcgttca acggatcccg tagcgaccct
      361 cgcgcaatcg cctacgcgcg agcgaccatc gacctggcat cccgggacgt ccgcatcgaa
      421 gacgtgccgt gccggaacct cgaggacgtg ctgggaggat tcgggcgatc gttccagatc
      481 ctggcggagc ggaccatcac gcacgcgttc acgcccgaca atccgctcat cgtcaacacc
      541 ggcgtgctga cggcgtcgaa cgtcatgacg gggctccgga cgtatttctc ggcctacagt
      601 ccgctcaagg tctccaacaa gggcctgccc gccgcgatct ggtcggccgg cagctgcaag
      661 ttcggcccga agctcaaatg gaccgggctc gacgaggtga ttctcgaggg ccgggccgac
      721 gtgcctgtgt atctggtgat ccggcacggc gagcaaggcc cccggattgc gttcgagccc
      781 gccggcgatc tgctcgggct cacctgccac gagaagatca tgtggctgcg cgatcggtac
      841 gccgacgcgc acttcgcggc gatcggcccc gccggcgagc actacgagga ctgctacatg
      901 gcgtcggtgg cactgagcac cgaaaaccag ctcaggtcgg gcgacgacaa gtgccgatgg
      961 gccggccgcg gcggcatggg cgccatcatg gggtacaagc gcgtgctggc gatcgtcgcg
     1021 caggcgcccg accgcatcgc gaagctgaag ccggagatcc gcgacatcaa caaggtggcg
     1081 tcgacgggcc ccggatcgcg caagttccgc gagaaggaga aaggagggct cggcgggacc
     1141 tggtcgaact acgagccgct cgagaagttc cacttcgttc cccagaacaa cttccggccc
     1201 acgggcgacg gcaagccgga gctgatgttc cgcgatcacg tccagccgca gttcgtcgtc
     1261 aaggccgagt cgtgcttccg gtgcggcatc aactgccaca agaacgtgta cgagaagaac
     1321 gccgacggga cgcgcggcgc cttcctggcc aagttcgact acgagccggt gaacctgctg
     1381 tcgaccaaca tcgggatcca cgatccgcgc aaggccgcca cgctcatcag cctggtcgac
     1441 cggctgggga tggacaacat ctcgctgggc acgaccatct cgtacgtgat ggactacaac
     1501 gaccgccatc cggacgcgcc aatcctgaac ggcgcgaagt tcggggactt cgacaaggcg
     1561 cgcgagctca tcgagggcgc ggccaacggg cagtttccgg aggtgggccg cggcgtcaag
     1621 cggctgtcgg agcaactcgg ggagacgggg tacgccatgc actgcaaggg tctggagttt
     1681 cccgcatatc agcctgatac caatcccggc tatccgtggg cgatcgcggg cggacacatg
     1741 tcgatggcca cctacatggc gctggcgctc gagggcaaca cctcgctcga ctattgggcc
     1801 acgctgatca ccgaacgtgg cctgtatttc gtgcgcgacg acctgcttgg cctgtgcaag
     1861 tttgccggcg tcaccgggga ccacgccgtc gagggcctga agcacgaaat cgggttcgag
     1921 gtgacgcctg cggagctgca ggccgccgtg cggcgcgcct tcgtccgcgc gttatggctc
     1981 gagcgcaagc aggggtacga tcgatcggac tacacgctgc cgtcggaggt gttcgatcgg
     2041 cccaacccca atctcggcgt acccccattc gtgacccgcg agttcttctc gcagctgagc
     2101 gatcgggtct gggcggtctt cgatcgggag atcgacgaga tcgcgaagac ctaggatgcc
     2161 ccggatccgt ttgacgaacc tcttcgccga gcgcatcggc ggcgtcaacg ccgttgacgt
     2221 gccggcgacg acggtcgagt cggcgctgcg cgcgctcacc gatcgacatc ccagcctgac
     2281 gccgctcgtc tgggcgaagg acggcgcgct caatcccgtc atggtgatgt tcctgaacga
     2341 ccgccagctc gccgccgcgg agatcgggac ccccgttggg cccggcgatg agatcgacct
     2401 cgtgcctgcg atcgaaggcg ggtgctcatg accccgtgat cgtttcgatc gacttcttga
     2461 gatcggccag atcgtcgctg agcacatggc ggaccttgcc cccaatcgcc cactcgagcg
     2521 gcagcagcac cgccggcggc agcccttcgg ggtgcagctc gaactcaatg ctgaccttcg
     2581 taccggcggg caccggctcg aacgtgaaga tggcatcgat gcgcacgcca ctcccaagaa
     2641 ctcccccttt gtagtgggtc accgtgtagg tgcgattcag ctcgaacgac gagatctcca
     2701 tggtcgcgtc gtcgagtcgt cccatgactt ctcgggtctc ggtccatcga taacccaggt
     2761 tgaacgggcc cgtggacagg acttcgatct tcttgatccc ggagacatgc tccgccgcat
     2821 gctcgatgtc cgtgaaaagc tcgaagacgc ggtcgactgg agcctttact tcggtggaaa
     2881 ccttgacttt cgccatcgtg aacctcccgc ggcggatgtg acagtggagc cgtctggcgc
     2941 tgttcccctg aatgaccaga caccggcgac cagggccagg ccgcccgctt cgaggacgcg
     3001 cgtcactatg agatcgagcg tgcccttcat ggcaatgaac gcggccagcg attcacggac
     3061 cctctggaca cgacatcctc cctctctgag ttgcccctac gggtttccga accgctccag
     3121 accggcgtgg tcaggacgat cgccacccgg attgatccac cctagtcctg cagggcggcg
     3181 cagctctggg cgagcggcaa gagaccgacg cccgtggtgt gaagacgtgt caacagcgat
     3241 gctgcgagct gagggtctga cgcgtgaatg tacagaccaa cctggtatgt ctgcggcaca
     3301 taatcaggat atcagcaacg ctccacagcc tcgtggaact tcagcatgtt ctcgccatcg
     3361 tctctgctgc tcgttcacct cgtcggcctg gcaatagggc tgggcgcagc gaccacgaag
     3421 ctggttctgc tgttccagtg tcgcgccgat ggtgcgttcg tgccgaccta cctggccgtc
     3481 gtcaggccaa tcacgcggca aatcatcctg ggactcgtgc tgctgctcct ttcgggcatc
     3541 ggattcatgg ctatcgggca ctcgttcacg cctcgactcg ttacaaagct ggtgctggtc
     3601 tcggcgatat tcgtactcgg gccggtcatc gacaacgcgg tcgaaccgag gttccgcgtc
     3661 ctggctcctc gaccggggac cgaggcgtct ccggaatttg ttcgcgtgcg gacacagtac
     3721 ctcgcactcg agctgctggc cactgggttg ttctacgcgg tcgtcatcat ctgggtcgtg
     3781 ttctagtcta gtgtcaggac acgctcggtg cgaacagtcg agggctcggc gcgctgcgac
     3841 aatcatcgca agtagcatcc agatcgaggc gcggggtagt ctaagatcgt ggtcacgtca
     3901 tgaagccgag cacgagttcg aagtcgaacg cgaaatctat cctgcgcttt catcatgtcc
     3961 tcgttcccac cgatctgacg gaacggactg agaaggcact ggagctggcc gacaagctga
     4021 cggtctctga tacgtcccgc atcacattga tgcacgtcat cgagacaatc gagggaatgc
     4081 agtttgacga actgaaaccc ttttacgatc gcctggaacg aaaagcccgt accacgatga
     4141 cccggttcgc tcgacgcgtc ccctccatgc gtgtgaagaa tgtgttgatc tatgggagac
     4201 gtgccgaaga gattgtgaag tttgcggcag ccaacgacgt cgatctgatc gtcctcgcct
     4261 cgcaccgtgt cgcgccctcg acagtgaacc gcgactgggg cac
//
LOCUS       DBNGSH010000618         7585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4307025, whole genome shotgun sequence.
ACCESSION   DBNGSH010000618 DBNGSH010000000
VERSION     DBNGSH010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4307025"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3086)
                     /locus_tag="AC16CY_14270"
     CDS             complement(<1..3086)
                     /locus_tag="AC16CY_14270"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3355011.1"
                     /translation="MRHITKALLVAAVVLSPAIAHAQATLAGTLRDASGGVLPGVTVE
                     ASSPALLEKARSSVTDSSGQYNIPSLPPGTYSVSFQLAGFNTVVRPGIEIFGAGVITV
                     NADMRVGGVQETITVTGESPVVDIQSTRRETVLDDEVISSIPASRGYNAILTAVPSVT
                     GGSQNVDLNPTMRIFTSHGGRGNEGRVQVDGLNVGAAFNGGGVSGFTMDTTNAAEMNF
                     TISGGLGEAETGGINLNIVPKTGGNTFAGTLFTSFAGNWSQGTNIDAELESYGVTVPA
                     DIYKVWDYSASFGGPIVRDRLWFLGSYRDFANHQAAPGVFGNANAGLPLSDPRAFTYA
                     RDESVAGRNISGKTIATIRLTGQVTERNKVGFYLDSQWNCNQTPYALDAAEGCRQRET
                     EWMGSGFGNQSVEAASLYQDGYERVMQFTWTSPVTNKLLLEAGESTYISRWGWMDPPG
                     AIKDRPQVAIGSAFGTTYNSVDNFFRNYQSPNVWRASASYVTGAHSMKFGYQGAHLIE
                     ETNDFANDTALTYVISTATQRPTSLTMRIAPTEMSNRTQYASLYAQDQWTFNRVTLQG
                     AVRYDRAWSWYTDTKNGAPQAGPFNPAPIVFGGGTMVDAYNDITPRVGAAWDVRGDGK
                     TSVRVNLGKYLQSANNQENYTIANPALDGRNGRRGPTFHTTTTRSWTDNDLDWAADCD
                     LMNPAAQSPATTGSIDTCGGWSDLSFGNPAANTRINPDVLKGWGVRPYDWQFGVSVQQ
                     EIFPRTSIEVGYHRRWFGNFFYTDNQAVTPADFSQATFNAPNDARLPGGGGFPVTVYD
                     INPVLQDGRPGFGQTRLANGSSALYYTFGSELGDESAYWQGFDVTVNARMANGLVFQG
                     GTSTGRGYRDQCEIWAAAPELFAPVTGAAKTQYDACSWTEPWLTQFRGLATYTIPKID
                     VLVSGVFQFKPNATTGPTDTTVATNGTSLAANYNVTNALIEGDLGRFFTGTTAPAQSR
                     TVNILTPGELYARRINQVDLRVAKVLRFSSNRLNVGFDLYNLFNSNPGLTFNQAYS"
     gene            complement(3414..5609)
                     /locus_tag="AC16CY_14275"
     CDS             complement(3414..5609)
                     /locus_tag="AC16CY_14275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3355012.1"
                     /translation="MTSTLLDRRSFLRVTAVAGGGMMIALHLDPAELLAQGPPGAQGF
                     DALAFVKIAADGTVTIMNKNPEIGQGVMNSLPMIIADELDVDWSSVRVQQAAVDGSKF
                     GTQFAGGSLSTPMNWDPLRQVGAACRQMILSAAAARWSVPVAELTTRSGRVMHTASNR
                     TGGYGEFAAAAAALPVPPLGKVPLKDPKDYRIIGTSQRGVDTAAITTGKPIFSIDFAL
                     PGMLYAVYEKAPVFGAKVATSNVDDLKKLPGVRHAFVIEGGNNLQELVGGVAIVADSW
                     WLAQSARKQLKVTWAEHPTSSQSSVGFQARADELGKGAYTTRLRTDGDVDAALAGAAK
                     VVEAAYMYPFIPHSPLEPENAVAQFQNGKLEVWAPSQTPAGGVGVAARAAGIKDPDVT
                     LHMMKVGGGFGRRLTNDYVAEVAAIAKQVGGAPVKLLWTREDDMRHEMYRPGGYHYLK
                     GGVDANGKIVAWRNHFVTFGNGKQYASAANIPGSQFPALFVQNFDFGDTQMPLGVPTG
                     ALRAPGSHAYSYVFQGFIDELAVAAGKDPVQFRLDLLASQAYPSPEKGGDGFEAARMA
                     AVLKLVAEKAKWGTARPKGTGLGVAFQYSHRGYFAEVAEVSVDNLKRVKVNKVWVAAD
                     IGSQIINPMHAENLVQGGVVEGISHMMQEVTFKGGAAVEGNFNNVPLLRMAQAPAVET
                     HWIKSNNPPTGLGEPSLPPVLPAVANAIFAASGTRVRSLPLSKHGFRWA"
     gene            complement(5622..6110)
                     /locus_tag="AC16CY_14280"
     CDS             complement(5622..6110)
                     /locus_tag="AC16CY_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3355013.1"
                     /translation="MPFTLNVNGKSSTVDVPSDMPLLWVLRDVLNFKGTKFGCGIGQC
                     GACTVHIGGRAVRSCQTTVAQANGRITTIEGLSPDGSHPLQRAWQEIDVPQCGYCQAG
                     QIMSAAALLAQKPAPTDADINTAMNGNLCRCGTYVRIREAIHRAAALATRSTSTAQAG
                     TR"
     gene            6224..7264
                     /locus_tag="AC16CY_14285"
     CDS             6224..7264
                     /locus_tag="AC16CY_14285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR3355014.1"
                     /translation="MGRTSRAAILLVAALLLCSPGAPGRTDLRAQSDPAEAPQPFGDW
                     LAALMVEARYRGFSDALLNETVAEVRPLDRVLDNDRAQAELNPGFRRYLSTRLTRAMV
                     DRGRELSRQHASLLAGVEKEFQVQRRVLLAIWGLETRYGRITGNTPVFQALATLAWEP
                     RRATLFRGELFDALTMVQRGHIEASSMTGSWAGAMGQPQFMPSSYLKHAVDFDGDGRR
                     DIWRSTGDALASMANYLKAAGWNGQQTWGREVRLTSAARTSIEQSIPKRTEGCYARRN
                     MTERRPLTQWQKIGVRLVNGGALPAADLEAGLVDVGERTFLVYPNYDAILAYNCAHYY
                     ALTVALLSERLR"
BASE COUNT         1213 a         2523 c         2487 g         1362 t
ORIGIN      
        1 tccgaatacg cctggttgaa cgtcaggccc gggttgctgt tgaacaggtt gtagaggtcg
       61 aacccgacgt tgagccggtt gctcgagaag cgcagcacct tggccacacg cagatcgacc
      121 tggttgatcc ggcgggcata gagctcgccc ggtgtcagga tgttcaccgt cctggactgg
      181 gccggggctg tcgtgccggt gaagaaacgt ccgaggtcgc cttcaatcag cgcgttcgtg
      241 acgttgtagt tcgcggccag cgacgtcccg ttggtggcca cggtcgtatc ggtggggcct
      301 gtcgtggcgt tcggcttgaa ctggaacacg ccgctgacga gcacgtcgat cttcgggatc
      361 gtatacgtgg ccagcccgcg gaactgcgtg agccacggct cggtccagct gcacgcgtcg
      421 tactgtgtct tggcggcgcc agtcactggc gcgaacagct cgggtgctgc ggcccagatt
      481 tcgcactggt ctctgtaacc gcgtccggtg ctcgttccgc cctggaacac aaggccgttg
      541 gccattctcg cgttgacggt cacgtcgaag ccctgccagt acgccgactc gtccccgagt
      601 tcgctcccga aggtgtagta gagcgcggac gatccattgg caagcctggt ctgcccgaac
      661 ccggggcggc cgtcctgcag gaccggattg atgtcgtaca ccgtcacggg gaacccgccg
      721 ccgccgggca ggcgtgcgtc gttgggcgcg ttgaaggtcg cctgcgagaa gtccgccggc
      781 gtgaccgcct ggttgtccgt gtagaagaaa ttgccgaacc accggcgatg gtaacccacc
      841 tcaatcgacg tcctcgggaa gatctcctgc tggaccgaga caccaaactg ccagtcatag
      901 ggccgcacgc cccatccctt cagcacgtcc gggttgatcc gcgtgttggc cgcgggattg
      961 ccgaacgaga gatccgacca gcctccgcag gtgtcgatgg atcccgttgt ggccgggctc
     1021 tgagccgccg ggttcatcag gtcgcagtct gccgcccaat cgagatcgtt gtcggtccag
     1081 ctgcgggtcg tcgtggtgtg gaacgtcgga cccctgcggc cattccggcc gtcgagggcg
     1141 gggttggcga tcgtgtagtt ctcctggttg ttggcgctct ggaggtactt gcccaggttc
     1201 acgcgaaccg acgtcttgcc gtcgccccgc acgtcccacg ccgcacccac gcgcggcgtg
     1261 atgtcgttat acgcatcgac catcgtgccg ccgccgaaca cgatcggcgc cgggttgaag
     1321 ggaccggcct gaggcgcgcc gttcttggtg tcggtgtacc agctccacgc ccggtcgtat
     1381 cgcaccgcgc cctgcagcgt gacgcggttg aacgtccact ggtcctgcgc gtacaacgac
     1441 gcgtactgcg tgcggttgct catctcggtc ggagcaattc gcatcgtgag ggacgtcggc
     1501 cgctgcgtgg ccgtggagat gacgtaggtc agggccgtgt cgttggcgaa gtcgttcgtc
     1561 tcctcgatca ggtgcgcacc ctgatagccg aacttcatcg agtgggcgcc ggtcacgtag
     1621 gaggccgagg cgcgccagac gttcggggac tggtagtttc tgaagaagtt gtccacgctg
     1681 ttgtacgtgg tgccgaaggc gctgccgatg gccacctgcg gccggtcctt gatcgctccc
     1741 ggagggtcca tccagcccca ccggctgatg taggtggact cgcccgcttc gagcagcagc
     1801 ttgttggtga ccggcgacgt ccaggtgaac tgcatgacgc gctcgtagcc gtcctggtac
     1861 aacgatgccg cctcgaccga ctggttgccg aagccgctcc ccatccactc cgtttcacgc
     1921 tgccggcagc cttccgccgc gtccagcgcg tacggcgtct ggttgcagtt ccactgcgag
     1981 tccaggtaga agccgacctt gttgcgctcg gtgacctggc ccgtcaggcg gatggtggcg
     2041 atcgtcttcc cgctgatgtt ccgccccgcc acgctttcgt cgcgcgcgta ggtgaacgcc
     2101 cggggatcgg acagcggcag ccccgcgttc gcgttcccga acacgccggg cgcggcctga
     2161 tggttcgcga agtcgcgata gctcccgagg aaccagaggc ggtcgcgcac gatgggaccg
     2221 ccgaacgacg cgctgtagtc ccacaccttg tagatgtcgg ccggtaccgt cacgccgtag
     2281 ctctccagct ccgcgtcgat gttggtgccc tgcgaccagt tccccgcgaa gctggtgaag
     2341 agcgttccgg caaacgtgtt gccgcccgtc ttcggcacga tgttgaggtt gatgccgccg
     2401 gtctcggcct cgccgagccc gcccgagatc gtgaagttca tctcggctgc gttggtcgtg
     2461 tccatcgtga acccggagac gccgccgccg ttgaacgccg cgccgacgtt cagcccgtcc
     2521 acctgcacgc gaccctcgtt gccacgcccg ccgtggctcg tgaagatgcg catcgtcggg
     2581 ttgaggtcga cgttctggct tccacccgtc accgacggga ccgccgtcag gatggcgttg
     2641 tagccgcgcg aggccggaat cgaactgatc acttcgtcgt cgaggaccgt ttcgcgccgc
     2701 gtgctctgaa tgtcaaccac gggcgactcg ccggtgaccg tgatcgtttc ctgcacgccg
     2761 ccgacccgca tgtcggcgtt caccgtgatg acgccggcgc cgaagatctc gatgcccgga
     2821 cgcacgaccg tgttgaagcc cgcgagctgg aagctgacgg aataggtccc cgggggaaga
     2881 ctcgggatgt tgtactggcc cgatgagtct gtgacgctcg agcgtgcctt ctcgagcaac
     2941 gccgggctcg aggcttctac agttacgccg gggaggactc cccccgacgc gtcacgcagc
     3001 gttcccgcca gcgtggcctg ggcatgtgcg atggcgggcg acaggacgac tgccgcaacc
     3061 agcaatgcct tcgtgatgtg gcgcatagct tctcctccgc gaacaagttg caacgcctac
     3121 tcccgtaact gattgactgc gcgcccctcg acgcgaccga agccggggct acctgttccc
     3181 agtcaatggg ttacgcggtc ttgccttata gcaattccgc ggccgtcgtc gtgttgcgtg
     3241 gacgccctgt agtatcaaca ggttactcgg ggtgcctgcc gcaatattcc aaatcggggg
     3301 aggtttggtt ccggaaagtt ggatcggggg gcaactccca acctccaaat cccaactccc
     3361 aagaacggct tgggggttgg gggttgggtg ttgggagttg ggagttcaga gccttacgcc
     3421 cagcggaagc cgtgcttcga cagcggcagc gaccgcacgc gcgtgcccga cgcggcgaag
     3481 atggcgttgg ccacggccgg caggaccggc ggcagcgacg gctcaccaag accggtcggc
     3541 gggttgttcg acttgatcca gtgggtctcg accgcggggg cctgcgccat ccgcagcagc
     3601 ggcacgttgt tgaagttgcc ctctactgcc gccccaccct tgaaggtgac ctcctgcatc
     3661 atgtggctga tgccctcgac cacgccgccc tgaaccaggt tctcggcgtg catcgggttg
     3721 atgatctggc tgccgatgtc ggcggccacc cagaccttgt tcacctttac gcgcttgagg
     3781 ttgtccacag acacctcggc gacctccgcg aagtagcccc ggtggctgta ctggaatgcc
     3841 acgccaagac cggtgccctt cggccgcgcc gtgccccact tcgccttctc ggcgacgagc
     3901 ttcagcacag cggccatgcg cgccgcctcg aatccgtcac cgcccttctc gggcgacgga
     3961 taggcctgcg acgcaagcag atccagccgg aactgcaccg gatccttgcc ggcggcaacg
     4021 gccagctcgt cgatgaaccc ctggaagacg taggagtacg cgtggctccc gggcgcgcgc
     4081 agcgcgcccg tgggcacccc gagcggcatc tgcgtatcgc cgaagtcgaa gttctgcacg
     4141 aagagcgcag ggaactgcga cccggggatg ttcgccgccg aggcgtactg cttgccgttg
     4201 ccgaaggtca cgaagtgatt gcgccaggcg acgatcttgc cgttcgcgtc gacgccgccc
     4261 ttcaggtagt gatacccgcc gggccggtac atctcatgac gcatgtcgtc ttcgcgcgtc
     4321 cacaggagct tgaccggcgc gcctccgacc tgcttcgcga tggccgcgac ttcagcgacg
     4381 tagtcgttcg tcaggcggcg cccgaagccg ccgccgacct tcatcatgtg gagcgtgacg
     4441 tcgggatcct tgatcccggc cgcacgcgcg gcgacgccca caccgcccgc cggcgtctgg
     4501 ctgggcgccc agacctcgag cttcccgttc tgaaactgcg caacagcgtt ctccggctcg
     4561 agcggcgagt gcgggatgaa cggatacatg taggcggcct cgacgacttt cgcggcgccg
     4621 gcaagcgccg cgtccacgtc gccgtcggtg cgcagcctgg tcgtgtacgc gcccttgccg
     4681 agctcgtcgg ccctggcctg gaagcccacg ctgctctgcg acgaggtcgg gtgctcggcc
     4741 cacgtcacct tcagctgttt gcgcgccgac tgcgcgagcc accagctgtc ggcgacgatc
     4801 gccactccac cgacgagctc ctgcaggttg ttgccgccct cgatgacgaa ggcgtgcctg
     4861 acgccgggca gcttcttgag gtcgtcgacg ttcgatgtcg ccaccttcgc gccgaacacc
     4921 ggcgccttct cgtagaccgc atacagcatt ccgggcagcg cgaagtcgat gctgaagatc
     4981 ggcttgcccg tcgtgatggc ggcggtgtcg acgccgcgct gcgacgtgcc gatgatcctg
     5041 tagtccttcg gatccttcag cgggaccttg ccaagcggcg gcaccggaag cgcggcagcg
     5101 gcagcagcga actcgccgta gccgccggtc cgattgctcg ccgtgtgcat cacgcggccc
     5161 gagcgcgtcg tgagctcggc cacgggcacg ctccagcgtg cggccgcggc cgacaggatc
     5221 atctgacggc aggcagcgcc gacttgtcgc agcggatccc agttcatcgg cgtcgagagg
     5281 cttccgccag cgaactgcgt accgaacttc gatccatcga cggccgcctg ctggaccctc
     5341 acgctgctcc agtccacgtc gagctcgtcg gcgatgatca tcggcagcga attcatcacc
     5401 ccctggccaa tctcggggtt cttgttcatg atcgtcaccg tgccgtcggc ggcgatcttc
     5461 acgaaggcca gcgcgtcgaa gccctgggcc cccggcgggc cctgcgcgag cagttcagcg
     5521 gggtccaggt gcagcgcgat catcattccg ccgccggcga cggcggtgac gcgcaggaag
     5581 gagcgacggt cgagaagtgt tgacgtcacg gcttcgtctc cttaccgggt cccggcctgc
     5641 gccgtggacg tggaacgcgt cgcgagcgcc gcggcgcggt gaatcgcctc gcgaatccgc
     5701 acgtaggtcc cgcagcggca cagattgccg ttcatcgcgg tgttgatgtc ggcatcggtc
     5761 ggcgccggct tctgcgcgag cagcgcggcg gccgacatga tctggcccgc ctggcagtag
     5821 ccgcactgcg gcacgtcgat ctcctgccat gcgcgctgca acgggtgcga cccgtccgga
     5881 gacagtcctt cgattgtggt gatccggccg ttggcctgag ccaccgtggt ctgacaggag
     5941 cgcacggccc gcccaccgat gtgcacggta cacgcgccgc actggccgat gccgcagccg
     6001 aacttggttc ccttgaagtt caggacgtcc ctgagcaccc acagcagggg catgtcagag
     6061 gggacatcga ccgtggagct cttgccgttg acgttcaatg tgaaaggcat ggcgttaact
     6121 ctctcgaaag taagggagat cgcttgggga aacgctgcgt cgttattaga agggagtcgc
     6181 cgcggaaatg caagcaagga gataatgagc cgataagaag ctaatgggac ggacgagtcg
     6241 ggccgcgata ctcctcgtcg cagccctttt gttgtgctct cctggcgcgc ccggccgtac
     6301 ggacctccgc gcccagagcg accccgccga ggcgccgcag ccgttcggcg actggctcgc
     6361 cgcgctgatg gtcgaggcac gctaccgcgg cttcagcgac gcgctgctca acgagaccgt
     6421 cgccgaggtg cgtccgctcg accgggtgct cgacaacgat cgggcccagg ccgagctcaa
     6481 ccccgggttc cggcgctacc tgtcaacccg ccttaccagg gcgatggtgg accgcgggcg
     6541 cgagctctcg cgccagcacg catcgctgct cgcgggagtc gagaaggaat tccaggtcca
     6601 gcgccgcgtt ctgctcgcca tctggggcct cgaaaccagg tacggccgta tcaccggcaa
     6661 cacgcctgtc ttccaggcgc tggcgacgct ggcctgggaa ccgcgccgtg ccaccttgtt
     6721 ccgcggcgag ctgttcgacg cgctgacgat ggtccagcgc gggcatatcg aagcctcgtc
     6781 gatgaccggt tcgtgggcag gggcgatggg ccagccgcag ttcatgccat cgagctactt
     6841 gaagcacgcc gtcgatttcg acggcgacgg ccggcgcgac atctggaggt caaccgggga
     6901 cgcactcgcg tcgatggcca actacctgaa ggcggccggc tggaacgggc agcagacctg
     6961 gggacgcgag gtacgcctga cctccgccgc gcgcaccagc attgaacagt cgatcccgaa
     7021 gcgcacggaa ggctgctacg cgcgccgcaa catgaccgag cggcgcccgc tcacccaatg
     7081 gcagaagatc ggcgtgcgcc tcgtcaacgg cggcgccctc cccgccgcgg atctcgaggc
     7141 gggactggtc gatgtcggcg agcggacctt cctggtgtac ccgaactacg acgccatcct
     7201 cgcgtacaac tgcgcgcact actacgcgct caccgtcgcg cttctgtcgg agagactccg
     7261 gtagaacagc gctcgaaaga ccactgacgt taaccacaga gacacagagg acacagagca
     7321 ttctttctcg acgaaggcgt ggcgagacat tgcctctgtg ttcgctgagt ctctgtggtt
     7381 ggcgttcgtg aagttcaacc cgtcacgtag tacgattagc ggttcaagga ggccccacac
     7441 acgatgtcca gtaccgcgta cgagagcgcc acactgttga tccgcctgta cgagctgcgc
     7501 cgcgagccga caatgcgcga ggcgcgcaac tggtttgcgc gcgaattcaa tccggccagc
     7561 atcgacgagg tgatgcaagc gctga
//
LOCUS       DBNGSH010000619         6822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_232036, whole genome shotgun sequence.
ACCESSION   DBNGSH010000619 DBNGSH010000000
VERSION     DBNGSH010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_232036"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="AC16CY_14290"
     CDS             complement(<1..260)
                     /locus_tag="AC16CY_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355015.1"
                     /translation="MTTVSWLDWKLGARLLLKYPALTIIGGLSLAAAIAIGVVGMEVA
                     DELLYKRLPFDDGGRVVRLETRDVAASRVEPRVLHDFAIWRRS"
     gene            complement(367..1833)
                     /locus_tag="AC16CY_14295"
     CDS             complement(367..1833)
                     /locus_tag="AC16CY_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3355016.1"
                     /translation="MTWFTSTRPPRVAIVVLLLVAAASFFAYHRLLREEPAPYFESAE
                     DHFLYGSIGTEADRGLPYWIWLVLPRVFPEYLPRPGGYASLGVLARDGQEMPIGLSKV
                     TIGVPRVGINCAMCHTASVRASRGDVPVIYPGAPAHQTGAQEYKRFLIACAADPRFTA
                     GTILGEIAKNVRLSLLDRLLYRFAIIPGTRRALLRLRDDDVPLTRRPDSGRGRADVFN
                     PVKFTILGQPIDETIGSADMMPTWNLARAERTSFHWDGLNTSLREAVASSAVNEGATV
                     WWLDRDAVKWDRTEAGRQSSLRRVMEYISALKPPKYPFPIDAKLSAAGAVTFASACAR
                     CHGAGGNQTGSVIPFDEVGTDRSRLDAWTSGAADKYNAYGDRHDWKFSGFRETNGYLA
                     APLEGTWLTAPYLHNGSVPTLTDLLEPPESRPRRFWRGYDVYDPARVGFESAGEDAER
                     AGTVFDVTQPGNGNAGHTYGVTLTPGEKRALLEYLKTL"
     gene            complement(1837..3327)
                     /locus_tag="AC16CY_14300"
     CDS             complement(1837..3327)
                     /locus_tag="AC16CY_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3355017.1"
                     /translation="MTSRLRAVLRALEVAFASLVILGVVWVLVALAIDRPTTYDDIEE
                     HFKYGSIGSEPGGSVLQTVGGVLPPYWIFKSLPSICSDKLPRGYETFGFITEPGKDLP
                     IGVSRRRRLGGDQVGLNCAVCHTSTVRDAPGAAPRIVLGMPAHRLDLQGFVQFVLDCT
                     LDSRLTPDAIRGRLPDRGGPSLFERALLRLGLVDRLKITVLNLRNRIAPALAAELPRW
                     GRGRVDTFNPYKSIQFNWDLSKLPPGELIGASDFPSIWNQKPREGMHLHWDGDNDSVD
                     ERNLSAALGAGVTPVTVDHDNLKRVRDWIWTLPPPKYPYAIDRALAARGEPVYQKLCT
                     NCHGDHRFRDGVITGSQIGRVVPLTEIGTDPYRLNSYTVAFAANQYSLYPDSEYRFSH
                     FRKTNGYANHPLDGIWLRAPYLHNGSVPTLRDLLEPPERRPKVFYRGYDVFDQANVGF
                     VSDGPEAQGYSRYDTSLPGNGNGGHVYGATLPEEDKRAIVEYLKTF"
     gene            3514..5970
                     /locus_tag="AC16CY_14305"
     CDS             3514..5970
                     /locus_tag="AC16CY_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355018.1"
                     /translation="MSSPRMAAAFFTIVVLAAVSLTGCGSDGPEPGTVLDEATRAKRT
                     ADTFAAADEDYFREMDGGLKFTREQVQGRNMWMVWTGGNDRLWDTLSLESLGSLDFLK
                     ILSSHPTQKYYSRDNRWNYLGLVNEPCFRRATGPDPNRYGLWLDVRDPKCPPDPFADA
                     DKYKGVQVGARGRTVPVGSYYGEPSGVVGLRLFPNPDFDEDARRKWDSERYYRDESYY
                     FSRDIVRPYRVGMSCAFCHVGPNPVRPPADPENPAWASLSSNVGAQFFWWDRVFDWRG
                     LSNETSFFYQALHTSLPGTLDTSLVSTDNINNPRTMNAVYLLGPRMGLAKRWGKETLA
                     GGGLDNKQFNEFVPASDPLAQFFTPPATTWTPRVLKDGSDSVGALGALNRVYLNIGLF
                     SEEWLLHFRPLIGGQPITPIKIADARKNSVYWQVTEKQTPYMAAFFLASTDPHYLRDA
                     PGGPKFLTEAADSLGRGKTVFADRCARCHSSKLPELPKGLDLENCNGKDYLGCWDKFW
                     AWTKTDSFRTPMRKMVLANDFLRDNFLSTELRIPSTLMQTNICSPIGTNAIEGNIWDN
                     FSSRSYKDLPSVGSVRLRHPLSGVEYSYTLKGGGRGFTRPASLVSLWSTAPFLQNNSV
                     GPFDPSPSVEARMRVFQASIEQMLWPERRRTDELFTSLKIDGPGVGWIQRTTTDSFIR
                     VPKGYIPGYLRPVLGIGQRFFPMFVRDGQLEIGPIPKGTPVSLVTSMDILGADLPEAE
                     RDQHREDLKNLLKQIKRELKRGNDVFGNRNIMETMLKMSNCPDLVINKGHYFGTNLFT
                     EEPGLSDAEKRDLIGFLKTM"
     gene            5981..>6822
                     /locus_tag="AC16CY_14310"
     CDS             5981..>6822
                     /locus_tag="AC16CY_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR3355019.1"
                     /translation="MPEATPRPATYDADYIVVGSGAGGGTLAARLAESGFRVLLLEAG
                     GDPRKSIGATPQTPGVNTLPDDYDVPAFHPLSTENDGMRWDFFVRHYEDQARQERDPK
                     YVASADGKPVNGVLYPRAGTLGGCTAHNALILVYPHNADWNQLADLTGDRTWRAENMR
                     QYFERLERCTHRPAERALSRLGANPSRHGWSGWLQTETPDPKVVFKDDGLREAFLESA
                     RAVLRNPELAITDADRRARFESQLDPNDWRVVSEDAIGLRYMPLTTKDHRRVGARERV
                     VDVET"
BASE COUNT         1205 a         2298 c         2226 g         1093 t
ORIGIN      
        1 gaccgccgcc agatcgcgaa atcgtggagc accctcggct ccacgcgtga cgccgcgacg
       61 tcacgcgtct ccaggcgcac gacccggccg ccgtcgtcga acggcagccg tttgtacagc
      121 agttcgtcgg cgacttccat gccgaccacg ccgatcgcga tggccgccgc cagcgagagg
      181 cccccgatga tcgtgagcgc gggatatttc agcagcaggc gcgcgccaag cttccagtcg
      241 agccaagaga ccgtcgtcat cggatagaag gatcccccgg cggaccgtcc ggtgtcttca
      301 cgtggaagtg acctttccgt gagattccgg tgagcgtgcc tgcggggcct acgaaatgga
      361 cttggattag agcgttttca agtactcgag gagcgcccgc ttctctcccg gagtcagcgt
      421 gacgccgtag gtgtggccgg cattcccgtt gcccggttgc gtgacgtcga agaccgtgcc
      481 ggccctttcc gcgtcctcgc ccgcgctctc gaaaccgacg cgtgccgggt cgtacacgtc
      541 gtacccgcgc cagaatcgcc gcggccggct ctccggcggt tcgaggagat cggtcagggt
      601 ggggacagac ccgttgtgca ggtacggcgc cgtcaaccag gtgccttcaa gcggggcggc
      661 caggtacccg ttcgtctctc ggaagcccga gaacttccag tcgtgccggt ccccgtacgc
      721 gttgtacttg tccgccgccc cagacgtcca cgcatcgaga cggctgcggt cggtgccgac
      781 ttcgtcaaac ggaatcaccg acccggtctg gtttcccccc gccccatggc accgcgcgca
      841 cgccgaggca aaggtgacgg cgcccgccgc gctcagcttg gcgtcaatcg ggaacgggta
      901 tttcggaggc ttcagcgcgc tgatgtactc catcacgcgg cgaagactgg actgccgccc
      961 ggcctcggta cgatcccact tcacggcgtc ccggtccaac caccagaccg tggcgccctc
     1021 gttcacggcc gacgatgcca ctgcctcgcg cagcgaggtg ttcagcccgt cccagtgaaa
     1081 cgacgtccgc tcggcgcgcg cgaggttcca cgtcggcatc atgtcggcac tgccgatcgt
     1141 ctcgtcgatc ggctgtccga ggatcgtgaa cttgaccggg ttgaacacgt cggcccgtcc
     1201 gcggcccgag tcgggccggc gggtcagcgg gacgtcgtcg tcccgcagcc gcagcagggc
     1261 gcgccgcgtg ccgggaatga tggcgaagcg gtatagcagg cgatccagga gcgagagtcg
     1321 gacgttcttc gcgatctcgc cgagaatcgt gccggccgtg aagcgcggat cggcggcgca
     1381 ggcgatcagg aagcgcttgt actcctgtgc tcccgtctga tgcgcggggg caccgggata
     1441 gatcaccggc acgtcgcccc gcgacgctcg cacgctggcc gtgtggcaca tggcgcagtt
     1501 gatgccgacg cggggaactc cgatcgtgac cttcgacagc ccaatcggca tctcctgccc
     1561 gtcgcgcgca agcacgccca gcgatgcgta cccgcccggc cgcggcaggt actcgggaaa
     1621 gacccgcgga agcacgagcc agatccagta gggcagcccg cgatccgcct ccgtcccgat
     1681 cgacccgtac aggaagtgat cctcggcgga ctcgaaatac ggcgccggct cctcgcgcag
     1741 gagccggtgg taggcgaaga agctggctgc ggccacgagc agcagcacga cgatggcaac
     1801 gcgcggcgga cgagtcgaag tgaaccaggt catcgctcag aacgtcttga ggtattcgac
     1861 aatcgcacgc ttgtcctctt ccggaagcgt ggcgccgtag acgtgtccgc cgttgccgtt
     1921 gcctggcagg ctcgtgtcgt agcggctgta gccctgagct tcggggccgt ccgacacgaa
     1981 tccgacgttg gcctggtcga acacgtcgta gccgcgatag aacaccttcg gccgccgttc
     2041 cggcggctcg aggaggtcgc ggagtgtcgg taccgagccg ttgtgcagat agggggcgcg
     2101 aagccagatg ccgtcgagag gatgattcgc gtacccgttc gtcttgcgga agtgggagaa
     2161 ccggtactcc gaatcgggat agaggctgta ttgattggcg gcaaaggcca cggtgtagga
     2221 attgaggcga tacggatcgg tcccgatctc ggtgagtggc acgacgcggc cgatttgcga
     2281 cccggtgatc acgccgtcgc ggaatcgatg gtcgccgtgg caattcgtgc agagcttctg
     2341 atagaccggc tcgccgcgcg cggcgagcgc ccggtcgatc gcatacgggt acttcggcgg
     2401 cggtagcgtc cagatccagt ctcgcacgcg cttcaggttg tcgtgatcga cggtcaccgg
     2461 agtgacgccg gcgccaagcg ccgcactcag gttgcgttcg tccaccgaat cgttgtcgcc
     2521 gtcccagtgg aggtgcatgc cctcccgcgg cttctggttc cagatcgacg ggaagtccga
     2581 cgcgccgatg agctcgcccg gcggcagctt gctcagatcc cagttgaact ggattgactt
     2641 gtaagggttg aaggtgtcca cgcgcccccg gccccatcgc ggcagctcgg cggcaagcgc
     2701 cggcgcaatg cgattgcgga ggttcagcac cgtgatcttc agccgatcga caaggccaag
     2761 acgcaggagc gcgcgctcga agagcgacgg ccctcccctg tccggcagcc gtcccctgat
     2821 cgcatccggc gtcaaccggc tgtcgagcgt gcagtccagc acgaactgca cgaacccctg
     2881 caggtccagc cggtgcgcgg gcatgcccaa gacgatcctc ggcgccgccc cgggggcgtc
     2941 gcggacggtg ctcgtgtggc agacggcgca gttcaggccg acctggtctc caccgagtcg
     3001 cctgcgccgc gacacgccga tcggaaggtc ctttcccggc tcggtgatga acccgaacgt
     3061 ctcgtagccc cggggcagct tgtcgctgca gatcgacggc agcgatttga agatccagta
     3121 cggcggcagc acgccgccga ccgtctgaag gaccgagccg ccaggctcgc tgccgatcga
     3181 tccgtatttg aaatgttctt cgatgtcgtc gtacgtcgtc ggccggtcga tcgcgagcgc
     3241 gacgagaacc cacacaacgc ccaggatcac caagctggcg aaggcgacct ccagggcccg
     3301 gagcaccgcg cggagccggc tggtcacgtg cgcgacattc aaacacgacg gatccgatcg
     3361 cgccaagagg attcagcggc gctcgcagcc aaactctgcc gtagaaaatt gcgacggtcc
     3421 ctgtgcagaa tcgatcctca catcggctag actcctttcg cgcgcgatga cacaacacgc
     3481 gcgacatccc gtcactcgat caggggctat ctcatgtcat caccacgcat ggcagccgcc
     3541 ttcttcacca tcgtcgtgct ggcagccgtc tcgctgaccg gctgcggcag cgacggccca
     3601 gagccgggca ccgtgctgga cgaggccacg agggcgaagc gcaccgccga cacgttcgcc
     3661 gccgcggacg aagactactt ccgtgagatg gatggaggtc tcaagttcac gagggaacag
     3721 gtccaggggc gcaacatgtg gatggtgtgg accggcggca acgatcggct ctgggacacg
     3781 ctgtcgctcg aaagcctcgg ctcgctcgac ttcctcaaga tcttgtcgtc gcatccgacg
     3841 cagaagtact actcgcgaga caaccggtgg aactacctcg gcctggtcaa cgaaccgtgc
     3901 ttcaggcggg cgaccggacc ggacccgaat cgctacgggc tgtggctcga cgtgcgcgac
     3961 cccaagtgcc cgcccgatcc cttcgcggat gccgacaaat acaagggagt tcaggttggc
     4021 gcccggggcc ggacggtccc ggtgggctcg tattacggcg agccgtcggg cgtcgtgggc
     4081 ctcaggctgt tcccgaaccc cgatttcgac gaggacgccc gcaggaagtg ggattccgaa
     4141 cgctactacc gggacgagtc ttactacttc tcgcgcgaca tcgtccggcc gtatcgcgtc
     4201 ggcatgtcgt gcgcgttctg ccacgtcggc ccgaatccgg tcaggccgcc ggccgatccg
     4261 gagaaccccg cctgggcgag cctgagctcg aacgtgggcg cgcagttctt ctggtgggac
     4321 cgcgtgttcg actggcgggg cctgtcgaac gagaccagct tcttctacca ggccctgcac
     4381 acctcgcttc ccggcacgct cgacacctcg ctcgtgtcaa ccgacaacat caacaacccg
     4441 cggacgatga acgcggtcta cctgcttggt ccacgcatgg gcctggcgaa gcgctggggc
     4501 aaggagacgc tcgcgggcgg cgggctcgac aacaagcagt tcaacgagtt cgtgcccgcc
     4561 tccgatccgc tcgcgcagtt ttttacgccg cccgccacga cgtggacgcc gcgtgtcctc
     4621 aaggacggct ccgattcggt cggcgcgctg ggcgcgctca accgcgtcta cctgaacatc
     4681 gggctcttca gcgaggagtg gctgcttcat ttccggccgc tcatcggcgg ccagccgatc
     4741 acgcccatca agatcgccga cgcccggaag aactccgtgt attggcaggt caccgagaag
     4801 cagacaccgt acatggccgc gttcttcctg gcgagcaccg accctcacta cctcagggat
     4861 gcaccgggcg gccccaagtt cctgaccgaa gccgccgact cgctgggccg cggcaagacg
     4921 gtcttcgccg accgctgcgc gcgctgccac tcgagcaagc ttccggaact gcccaagggg
     4981 ctggacctcg agaactgcaa cggcaaggac tacctcggat gctgggacaa gttctgggcg
     5041 tggacgaaga ccgactcgtt caggacgccc atgcgcaaga tggtgctcgc caacgacttc
     5101 ctcagggaca acttcctgtc caccgagctg cgcatcccgt ccacgctcat gcagaccaac
     5161 atctgcagcc cgatcgggac caacgcgatc gagggaaaca tctgggacaa cttctcctcg
     5221 cggtcctaca aggacctgcc gtcggtaggc tccgtccggc tgcggcaccc gctcagcgga
     5281 gtcgagtaca gctatacgct gaagggcgga gggcgtggtt tcacgcgccc ggcgtcgctc
     5341 gtcagcctgt ggtccaccgc gcccttcctc cagaacaatt ccgtcggtcc gttcgacccg
     5401 agcccgtcgg tcgaggcgcg gatgcgcgtc ttccaggcgt cgatcgaaca gatgctgtgg
     5461 cccgaacgcc ggaggacgga cgagctcttc acgagcctga agatcgacgg gcccggcgtc
     5521 ggctggatcc agcggacgac gaccgacagc ttcatccgcg tgccgaaggg atacatcccc
     5581 ggctacctgc gtccggtgct gggaatcggg cagcgtttct tcccgatgtt cgtccgggac
     5641 ggccagctgg aaatcggtcc cattcccaag ggcacgccgg tcagcctcgt caccagcatg
     5701 gacatcctcg gggcggacct gcccgaggcc gaacgcgacc agcatcggga ggacctgaag
     5761 aacctgctca agcagatcaa gcgcgagctc aagcgcggca acgacgtgtt cggcaatcgc
     5821 aacatcatgg aaacgatgct caagatgagc aactgccccg acctggtcat caacaagggg
     5881 cattacttcg gcacgaacct cttcaccgaa gagccggggc tgagcgacgc cgagaagcgc
     5941 gacctgatcg gattcctgaa gacgatgtaa cggaggtgat gtgccggagg cgaccccacg
     6001 tcctgctacc tacgacgccg actacatcgt cgtcggctcg ggcgccggcg gcggcacgct
     6061 cgccgcgcgg ctggccgagt ccggctttcg cgtgctcctg ctcgaggcgg gaggtgaccc
     6121 gcggaagtcg atcggagcga cgccgcagac gcccggcgtc aacacgctgc ctgacgatta
     6181 cgacgtcccc gcgtttcacc cgctctcgac cgagaacgac gggatgcggt gggacttctt
     6241 cgtccgccat tacgaggatc aggccaggca ggaacgcgac ccgaaatacg tggcaagcgc
     6301 cgacggcaag ccggtgaacg gcgtgctcta tccgcgagcc ggcacgctcg gcgggtgcac
     6361 ggcccacaac gccttgattc tcgtctaccc acacaacgcg gactggaacc agctcgcgga
     6421 tctgacgggc gaccggacgt ggcgcgcgga gaacatgcgc cagtacttcg aacgcctgga
     6481 gcgctgcacg catcggccgg ccgagcgtgc gctgagccgc ctcggggcga atccgagccg
     6541 gcacgggtgg tccggctggc tgcagaccga gacgccggat ccgaaagtgg tgttcaagga
     6601 cgacggcctt cgcgaggcat tcctcgagtc ggcgcgagcg gtgctgcgca acccggagct
     6661 cgcgatcacc gacgccgacc ggcgcgcgcg cttcgaaagc cagctcgacc ccaacgactg
     6721 gcgggtcgtg agcgaggacg cgatcggcct tcgctacatg ccgctgacga cgaaggacca
     6781 tcgacgggtc ggcgcacgcg agcgggtcgt cgacgtcgag ac
//
LOCUS       DBNGSH010000620         8844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_62571, whole genome shotgun sequence.
ACCESSION   DBNGSH010000620 DBNGSH010000000
VERSION     DBNGSH010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_62571"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..2986)
                     /gene="uvrA"
                     /locus_tag="AC16CY_14315"
     CDS             complement(50..2986)
                     /gene="uvrA"
                     /locus_tag="AC16CY_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3355020.1"
                     /translation="MPDVTKPRSARNPIVVRGARTHNLKNVDVTLPAGTLAIITGVSG
                     SGKSSLAFDTIYAEGQRRYVESLSAYARQFLERMEKPDVDRIEGICPAIAIRQKNSVR
                     NPRSTVGTMTEIHDYMRLLFARVGRTFCRQCGQEVVRETAEVVARRLAELPEGTRLLI
                     GFAIPVVSITGPPPSSADGDNGDDADEDAEQPARNGADGVAETLDGLRRRGYGRLLID
                     GKAVVFEEVDTAALRSRSTLEVVVDRVRVEGDVRSRLTDSIETSYREGGGAAFAVVID
                     QASGPGAQAPEAPVRLEFSERFECRACGIGYETPQPRLFSFNNPFGACPTCHGFGNII
                     ELDMGLVVPDPSKTINQGAIEPWSKPHYRAQLAELKRAARKSGVRLDVPWEELTDEER
                     QFAVEGDGDDFEGVRGFFRWLERKKYKVHVRVFLSRYRGYLSCPDCGGARLRREARDV
                     RVGGRTIDEVSALTVEGGRGFFSGLQLTEKEAAVADKVLREIQRRLTFLSDVGLDYLT
                     LDRLSSTLSGGESQRINLATSLGSALVGTLYVLDEPSIGLHARDNQRLIEILRQLRDQ
                     GNTVLVVEHDEDMIRVADHVVDLGLGAGEQGGRVVYSGDLPGLLQDTRSLTAKYLRDE
                     LAIPVPSQRRKGIGQKIRLRGASEHNLKDIDVDIRLNTLTCVTGVSGSGKSTLVHDVL
                     YAAIKRAKGDWDRRVGTHTGLEGLEYITDVALVDQTPIGRTPRSNPVTYLKAFDPIRE
                     LFSLTKDARSRGLTASHFSFNVPGGRCEACQGEGEVRVEMQFLADVFVPCDQCDGKRF
                     KPRVLEVKYRGRSVHQVLEMTVREALAFFSTSPKVLRRLQVLDEIGLGYLRLGQPATT
                     LSGGEAQRVKIAAHLSSFGGERVLYILDEPTTGLHFDDIAKLLAAFRKLVEAGHTLLV
                     IEHNLDVVKTADYLIDLGPEGGDAGGRVVAAGTPEQVVQVEESHTGRALREVLAAGRS
                     NAYAAR"
     gene            3062..4786
                     /locus_tag="AC16CY_14320"
     CDS             3062..4786
                     /locus_tag="AC16CY_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3355021.1"
                     /translation="MPESGLILTLAGGLGAALLCGYVTQRLGLSPIVGYLLAGVLVGP
                     HTPGFIADPHLAEQLAEVGVILLMFGVGLQFHLKELLAVRRIAIPGAIAQSGVATALG
                     AFAAHAFGWTWPAAIVFGLALSVASTVVLVRVLSDSRQLHTQAGHIGVGWLVVQDVLT
                     VIVLVLLPALKGGSSPADVGMSIGLTLLKVAGLVAVAAIVGRRVIPIVLDRVAATRSR
                     ELFTLTVLALALGLAVGSAAVFGVSVALGAFLAGMVVGSSDYSLRAATDALPMRDAFA
                     VLFFVSVGMLLRPSALLAYPAFTVTALTIVIVCNPLTAAAVAWLMGYPIRTTATVAMS
                     LGQIGEFSFILVTLGRDLAIVPAEAVDVVVATAIISITLNPLAFRTIEPLAEKLGQLR
                     LFRRDDTEAADTGASSSLDPEGRAIVIGHGPTGRTVTRMLLENGISPTVVELNMSVVR
                     ELRDQGVSAVYGDAGRAETLISAGLPHAATLIVSGADTGSPGIIQSARSINPTAHIFV
                     RSPYLRDVPSLRRAGAERVFSGEGEVALAMTEAVLRRLGATPDQIDRERARVRQELMP
                     SDHDTPKQ"
     gene            complement(4813..5493)
                     /locus_tag="AC16CY_14325"
     CDS             complement(4813..5493)
                     /locus_tag="AC16CY_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006307 - DNA alkylation repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR3355022.1"
                     /translation="MHALVRAIRDALQQESDPARARGMQAYMKSAMPYRGVAGPVQKA
                     LWRRLFPAHPLSSRAEWRRVALELWRGAAFREERYAAVALTDLRAYAPYRTMAAIPLF
                     EEMIVTGAWWDYVDAVATHQLGDVLRAEPRPMSPLMRRWSRDRNMWKRRASILCQIRF
                     KKDTDLDLLYACIEPNMADREFFVRKAIGWALRQYAWVDPREVKRFVRTHKTRLSGLS
                     VREALKNV"
     gene            5649..6233
                     /locus_tag="AC16CY_14330"
     CDS             5649..6233
                     /locus_tag="AC16CY_14330"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3355023.1"
                     /translation="MRALITIGLAAALAVGVKAQSTELKVGDNAPDFSLQASDGKTYK
                     LSDFKGKQAVVVAWFPKAFTQGCTIECKSLAENGSKIKMYDVAYFMASVDSVEENTKF
                     AKATSVTLQGRGGGPSTVVEKKEADFPLLSDPTKEVATKYGVLMAGPGLANRWTFYID
                     KAGKIAYIDKAVRPPTSAEDMIAKLAELKVPMNH"
     gene            6372..>8844
                     /locus_tag="AC16CY_14335"
     CDS             6372..>8844
                     /locus_tag="AC16CY_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355024.1"
                     /translation="MTLSVGSRIGPYQVVSQLGQGGMGVVFRARDTRLERDAALKTLP
                     DVFAGDRDRLSRLQREAQVLASLNHPHIAQIYGLEEAGGACYIAMELVEGETLADRIV
                     HGPIPVDEVVRIARQIADALEAAHEKGVVHRDLKPANIKLTPDGRVKVLDFGLAKAID
                     HGAGTTAMSQSPTLSLAATQAGLVLGTAGYMSPEQAKGKPVDRRADIWAFGVVVYEML
                     AGRPAFSGETVSETLAHVMMKEPDWQAPSKAPAGLVHLLQRCLVKDPRDRLQAIGDAR
                     IELDEISRTSSEHRPSSPRPSERSRVLAWSLALLLVCSAAGAAAFVWWPREAAAPAPI
                     SRWTLQLREGESIAAGRVPGGLGIGRPSIALSPDDRYIVYVVERAGQTMLHVRATNEF
                     EGKPIPGTEGGFQPFFSPDGQWVGFFTDTKLKKAFLQGGSPVELCDAVHVYGASWGSD
                     DRIYFADREANRLTRIAASGGTPEVVANSSEVGTTLLWPHVLPGSKAVLFNSPFPGTI
                     NVLSLEDRRSRQLIAGASAVYAGSGHIVFSRGSDVLAAAFDLERVAITGPAVPVLENV
                     RAEANEASQFSISTRGTLAYVEGGFARHANLVWLDRQGRETPVDVPAQVYGSFTLSPD
                     GRRIAVIIPAQAEGQTTDIWIYDLERHNRTRLAARVQTNPVWAPDGRAVFLGLEQDGP
                     SNVYRLAADGTGVAERLTESRYSQYPQTISPDGKTLIYGEATASGDRDFWSLRLDGKG
                     KPQLYLQTPAREIFAAFSPDGRWLAYVNDAAGRYEVYVVAADGTGDRRLVSTEGGEEP
                     RWSARGDLFYRNGQQWMAVAVAPGAA"
BASE COUNT         1427 a         2966 c         2908 g         1543 t
ORIGIN      
        1 ctccgcagac tcgtagcggg tgtcctcaag aaatgactcc cgaccccatt tatctcgcgg
       61 catacgcgtt cgatcgcccc gcggccagca cttctcgaag cgcgcggccg gtgtgcgact
      121 cttcgacctg aaccacctgt tcgggagtcc cggcggccac gacacgtccg ccggcatcac
      181 cgccttcggg tccgagatcg atcaggtagt ccgccgtctt cacgacgtcg agattgtgct
      241 cgatgacgag cagcgtgtgc ccggcctcca cgagcttccg gaaggcagcg agcagcttcg
      301 cgatgtcgtc gaaatgcagg cccgtggtcg gctcgtccag gatgtagagc acgcgctcgc
      361 cgccgaagga cgacaggtgc gcggcaatct tgacccgctg cgcctccccg cccgacagcg
      421 tcgtcgccgg ctgtcccagc ctcagatagc cgagcccgat ctcgtcgagc acctgcaggc
      481 ggcgaagcac cttcggcgat gtgctgaaga acgccagcgc ctcgcgcacg gtcatctcga
      541 gcacctggtg tacggaacgc ccgcgatact tgacctcgag cacacgcggc ttgaaacgct
      601 tgccgtcgca ctggtcgcac ggcacgaaga cgtccgccag gaactgcatc tcgacgcgca
      661 cttcgccctc gccctggcac gcctcgcatc tgccgccggg gacgttgaac gaaaagtgac
      721 tggccgtcag tcccctcgag cgcgcgtcct tggtcagcga gaacagctcc cggatcggat
      781 cgaacgcctt caagtacgtg acggggttcg aacgcggcgt ccggccgatc ggcgtctgat
      841 cgaccagcgc cacgtcggtg atgtactcga ggccctccag cccggtgtgg gtcccgacgc
      901 ggcgatccca gtcgcccttc gcccgcttga tggctgcgta caggacgtcg tgcacgagcg
      961 tcgattttcc agacccgctc acaccggtca cgcaggtcag cgtgttgagc cggatgtcga
     1021 cgtcgatgtc cttgaggttg tgctcgctgg cgccccgtaa acggatcttc tggccgatgc
     1081 ccttgcggcg ctgggacggc accgggatag ccagctcgtc ccgcagatac ttcgcggtca
     1141 gcgacctcgt gtcctgcagc agtcccggca agtctcccga gtagacgacg cgccctccct
     1201 gctcgcccgc gccgagcccc agatcgacga cgtgatcggc gacgcggatc atgtcctcgt
     1261 cgtgctcgac gacgagcacc gtgttgccct ggtcgcgcag ctgccggagg atttcgatga
     1321 ggcgctggtt gtcgcgcgcg tgcaggccga tggaaggctc gtccagcacg tagagcgtgc
     1381 ccacgagcgc cgagccgagc gacgtcgcca ggttgatgcg ctgggactcg ccaccggaga
     1441 gcgtcgaaga cagccggtcg agcgtgaggt agtcgagccc gacgtcgctc aggaacgtca
     1501 accggcgctg gatttcccgc agcaccttgt cggcgacagc cgcctccttt tccgtgagct
     1561 gcagcccgga gaagaacccg cgtccacctt cgaccgtgag cgcggaaacc tcgtcgatcg
     1621 tgcggcctcc cacgcggaca tcccgggctt cgcggcgcag ccgggcgccg ccgcagtcgg
     1681 ggcaagacag gtagccgcga taccggctca gaaagacgcg gacgtgcacc ttgtatttct
     1741 tccgctcgag ccaccggaag aaccctctga cgccctcgaa gtcgtcgccg tccccttcga
     1801 cggcgaactg ccgttcctcg tcggtcagtt cctcccacgg cacgtcgagg cggacgcccg
     1861 acttgcgggc ggcgcgcttg agctcggcaa gctgcgcgcg gtaatgcggc ttgctccacg
     1921 gttcgatcgc cccctggttg atcgtcttcg acggatccgg aacgaccagg cccatgtcga
     1981 gttcgatgat gtttccgaat ccgtggcacg tggggcacgc accgaagggg ttgttgaacg
     2041 agaacagcct cggctgcggc gtctcgtagc cgatgccgca ggcgcggcac tcgaagcgtt
     2101 cggagaactc cagccggacc ggggcctctg gggcctgagc cccgggccct gaagcctgat
     2161 cgatcaccac ggcgaaggct gcccctccgc cctcccgata ggaggtctcg atcgagtcgg
     2221 tcaaccggct tcgcacgtcg ccctcgacgc gcacgcggtc cacgacgacc tcgagcgtcg
     2281 agcgcgaccg gagagccgcg gtatcgacct cttcgaagac gaccgccttt ccgtcgatca
     2341 gcagccggcc gtagcctcga cgccggaggc cgtcgagcgt ctcggcgacg ccgtcggctc
     2401 catttctcgc cggctgttcc gcatcctcgt cggcgtcgtc accgttgtca ccgtcggcgc
     2461 tcgacggagg cgggccggtg atcgagacga ccggtatggc gaacccgatc aggagccggg
     2521 ttccctcggg cagttccgcg agccgccgtg cgacgacctc ggccgtttcg cgcacgactt
     2581 cctgcccgca ctgccggcag aacgtgcggc ccacacgggc gaacaacagg cgcatgtagt
     2641 cgtgaatctc ggtcatcgtg cccacagtgg accggggatt ccgcacgctg ttcttctggc
     2701 ggatggcgat ggccgggcag atgccttcaa tgcgatcgac atcgggcttc tccatccgct
     2761 cgaggaactg gcgcgcgtaa gccgacagcg actccacgta gcggcgttga ccctcggcgt
     2821 agatcgtgtc gaaggcgaga gacgacttcc ccgagccgct cacgccggta atgatcgcca
     2881 gcgtgccggc tggcagcgtc acgtcaacgt tcttgaggtt gtgcgtccgc gcaccgcgaa
     2941 cgacgatcgg gttgcgggcg ctccgcggct tggtgacgtc aggcatcccc acggtggcga
     3001 attccttgtg gaccgtaagg gacccatgtt actacacgca accgaatacc attaggaaca
     3061 catgcccgaa tcgggactga ttctcacgct tgcgggcggc ctcggcgccg cgcttctatg
     3121 cgggtacgtc acgcagcgtc tcggcttgtc gccaatcgtc ggatatctgc tggccggagt
     3181 actggtcggg ccccatacgc ccgggttcat tgccgacccg cacctcgccg agcagctcgc
     3241 agaggtcggc gtcattctct tgatgttcgg cgtggggctg cagtttcacc tcaaggagct
     3301 ccttgccgtt cgccgtatcg cgattccggg agcgatcgcc cagagcgggg tggccacagc
     3361 tctcggagcg ttcgctgcgc atgccttcgg gtggacgtgg ccggccgcca tcgtcttcgg
     3421 cttggcgctt tccgtggcca gcacggtagt cctcgtccgg gtgctctcgg acagccgcca
     3481 actccacacg caagccggac acatcggcgt cggctggctc gtcgtccagg acgtgctcac
     3541 ggtgatcgtg ctggtgctgc tgccggcgct caagggcgga tcctctccgg ccgacgtggg
     3601 catgagcatc gggctgacac tgctcaaggt tgccggactc gtcgccgttg cggcgatcgt
     3661 gggtcgccgc gtgattccca tcgtcctcga ccgcgtcgca gccaccaggt ctcgggagct
     3721 cttcacgctc acggtgctcg cgctggcgct cgggttggcg gtcgggtcgg cggcggtgtt
     3781 tggcgtctcg gttgccctcg gtgcattcct ggcgggcatg gtcgtgggca gctccgacta
     3841 cagcctccgc gctgccaccg acgcactccc gatgcgcgac gcgttcgccg tgctgttctt
     3901 cgtgtcggtg ggcatgttgc tacgcccgtc ggcgctcctg gcgtacccgg cgttcaccgt
     3961 gactgcgctg acgatcgtca tcgtctgcaa cccgctcacc gcggcggccg tcgcctggct
     4021 gatgggctat cccattcgaa cgacggcgac tgtggcgatg tcgctggggc aaatcggcga
     4081 gttttcgttc atcctcgtga cgctcggccg agacctggct attgtgccgg ccgaagccgt
     4141 cgacgtggtc gtggccaccg caatcatctc gatcacgctc aacccgctcg ccttcaggac
     4201 gatcgagccg ctggcggaga agctcggcca gctgaggctg ttccgtcgtg acgacaccga
     4261 agccgccgat accggcgcct cgtcctcgct cgatccggaa ggccgggcga tcgtcatcgg
     4321 ccacgggccc accggccgca cggtgaccag gatgcttctc gagaacggca tttctccgac
     4381 ggtggtcgag ctcaacatgt ctgtggtgcg ggagctgcgc gatcagggcg tgtcggccgt
     4441 ctacggcgat gcgggccggg cggagacgct catctccgcg ggtcttcccc acgccgccac
     4501 gctgatcgtc agcggggccg ataccggctc gccgggcatc atccagagcg ccaggtcgat
     4561 caacccgacg gcgcacatct tcgtgcgcag tccgtacctg cgcgacgtcc cgtcacttcg
     4621 gcgggcgggc gccgagcggg tgttttcggg cgagggcgag gtggcgctgg ccatgaccga
     4681 agctgtgctg cgtcgtcttg gcgcgacccc cgatcagatc gatcgcgaac gtgcccgcgt
     4741 gcgccaggag ctcatgccaa gcgatcacga cacaccgaag cagtagaggc gggtcttcag
     4801 acccgcctgg cctcacacgt tcttgagagc ttccctcaca ctcagaccgc tgagccgtgt
     4861 cttgtgagtc cggacgaacc gcttgacctc gcgcggatcg acccacgcgt actgacgcag
     4921 cgcccacccg atggccttgc ggacgaagaa ttcccgatcg gccatgttcg gctcgatgca
     4981 cgcgtacagg agatccagat cggtgtcctt cttgaatcgg atctggcaga ggatggaggc
     5041 gcgtcgcttc cacatgttcc ggtctcgcga ccatcggcgc atcagaggtg acatcggccg
     5101 cggctccgca cggagcacat cgccgagctg atgcgtggcg actgcgtcca cgtaatccca
     5161 ccaggcgccc gtcacgatca tttcctcgaa cagcggaatg gcggccatcg tgcggtaagg
     5221 cgcgtacgcg cggaggtcgg tgagcgcgac ggccgcgtag cgctcctccc gaaacgccgc
     5281 ccccctccag agctcgagcg ccacgcggcg ccactccgcc cgggacgaca gcggatgagc
     5341 cggaaacagc cgccgccaca aggccttctg gaccggcccc gcgacgcccc gatagggcat
     5401 cgccgacttc atgtaggcct gcattccgcg ggccctggcc ggatcggatt cctgctggag
     5461 ggcgtcgcga attgcgcgga ccaaggcgtg catcgtagta ggcctcagga cccaggcctc
     5521 gggcctcagc ggcctcggtg aaccactaaa gctgaggcct gattcctggg cccttcttcc
     5581 ggttcggcag tatactcaag cgttcggaca cggacggtct gagggcgttt cggctaggag
     5641 ggtttctgat gcgcgcattg ataacgatag ggctggctgc ggcgctggcc gttggagtga
     5701 aggcccagag caccgagctg aaggtcgggg acaacgctcc cgatttcagc ctgcaggcgt
     5761 ccgacggcaa gacgtacaaa ctgtcggact tcaagggcaa gcaggcggtc gtcgtcgcct
     5821 ggttcccgaa ggcgttcacg cagggctgca cgatcgagtg caagtcgctc gcggagaacg
     5881 gcagcaagat caagatgtac gacgtggcgt acttcatggc cagcgtcgac agcgtcgaag
     5941 agaacaccaa gttcgccaag gcgacgagcg tgacgcttca gggccgcggc ggcggtccgt
     6001 cgacggtcgt cgagaagaag gaagccgact tcccgctcct cagcgatccg accaaggaag
     6061 tggcaacgaa gtacggcgtg ctgatggcgg ggcccggtct cgcgaaccgc tggacgttct
     6121 acatcgacaa agccggaaag attgcctaca tcgacaaggc ggttcgcccg ccgacgtcgg
     6181 ccgaggacat gattgccaag ctcgccgagc tcaaggttcc gatgaaccac tgacacgaca
     6241 agcgagtcgt tcacacttct cgcttctctc ttctcggttc gtgttcatgt tcgatgttcg
     6301 ggcgggccac tctggcccgc cctttttttt gtggccgtca tcatcgcgac gggcggctac
     6361 aatcgcgctg aatgaccctc tcggtcggct cgagaatcgg cccttaccag gtggtgtccc
     6421 aactcggtca gggcgggatg ggggtcgtct ttcgcgctcg cgatacacgg ctcgagcggg
     6481 atgccgcgct caagacgctg ccagacgtct ttgccggcga ccgcgatcgc ctgtcgcgcc
     6541 tgcagcgcga agcgcaggtg ctcgcctcgc tcaaccaccc tcacatcgcg cagatctacg
     6601 ggctcgagga agccggcggc gcctgctaca tcgcgatgga gctcgtcgaa ggcgagacgc
     6661 tcgccgatcg gattgtgcat gggccgatcc cggttgacga ggtcgtccgg atcgcccggc
     6721 agatcgcgga cgccctggag gcggctcacg agaaaggtgt cgtgcaccgc gacttgaagc
     6781 ccgccaatat caagctgacg ccggacgggc gcgtcaaagt actcgacttc gggctggcga
     6841 aagcaatcga ccacggtgcc ggcacgacgg ccatgtcgca gtcgccgacg ctgagccttg
     6901 cggccacaca agccggcctc gttctcggga ccgcgggata catgtcgccc gagcaagcca
     6961 agggcaagcc ggtcgatcgc cgcgccgaca tctgggcatt tggcgttgtc gtgtacgaga
     7021 tgctcgcggg gcgtccggct ttttcgggcg aaacggtctc ggagacgctg gcgcacgtca
     7081 tgatgaagga gccggattgg caagcgccct ccaaggcgcc cgccgggctc gttcacctcc
     7141 tgcaacgctg tctcgtcaaa gacccgcggg atcgcctgca ggccattggc gacgcccgca
     7201 tcgagctcga tgagatctcg agaacgtcgt ccgagcaccg gccgtcgtcg ccgcgaccga
     7261 gcgagcgttc ccgtgtgctc gcctggagcc tcgccttgct tctcgtctgc tcggccgccg
     7321 gtgctgccgc gttcgtctgg tggcctcgcg aggcggctgc tccagcgcca atcagccgat
     7381 ggacgcttca gctgcgcgag ggcgagagca ttgccgctgg acgggtgccc ggcgggctcg
     7441 gcatcgggag accgtccatc gcactctcac ccgatgaccg ctacatcgtc tacgtcgtcg
     7501 agcgcgctgg acagacgatg ttgcacgtgc gcgcgaccaa tgagttcgag ggcaagccga
     7561 ttcccgggac cgaaggaggc ttccagccgt tcttttctcc tgacggccaa tgggtcggct
     7621 tcttcacgga taccaagctc aagaaagcgt tcctgcaagg cggatcgccc gtcgagttgt
     7681 gcgacgccgt tcacgtgtac ggcgcgagct ggggcagcga cgatcgcatc tacttcgccg
     7741 accgggaagc aaaccggctc actcgaatcg cggcgagcgg cgggacgccc gaggtggtgg
     7801 ccaactccag tgaggtcggc acgacgctgc tctggcccca tgtcctgccg ggttcgaagg
     7861 cggttctgtt caactcgccg tttcccggga ccatcaacgt cctgtcgttg gaggaccggc
     7921 gcagccgcca gctcatcgcc ggcgcgagcg ccgtatacgc ggggagcggc cacatcgtgt
     7981 tctcgcgcgg gagcgacgtc ctggcggcgg cgttcgatct cgagcgcgtg gcgataaccg
     8041 ggccggcggt gccggtgctg gagaacgtcc gcgccgaggc gaacgaggcc agccagttct
     8101 cgatttcgac ccgaggcacg cttgcgtacg tcgaaggagg tttcgcgcga cacgcgaacc
     8161 tcgtgtggct cgatcggcaa gggcgagaga ctccggtgga cgtgccggcc caggtctatg
     8221 gatcgttcac actctcgccg gacgggcgtc gcatcgcggt catcattccc gcgcaggcgg
     8281 agggccagac caccgacatc tggatttacg atctcgagcg acacaaccgg acgcggctcg
     8341 ccgcgcgcgt ccagaccaat ccggtttggg ctcctgacgg cagggccgtg tttctcggcc
     8401 tggagcagga cggcccgtcg aacgtctacc ggctggctgc cgacgggacc ggcgtggcgg
     8461 agcggctgac ggagagccgg tactcccagt atccacagac gatttcgccg gacggcaaga
     8521 cgctcatcta cggcgaagcc acggcgtcag gcgatcgcga tttctggtcg ctgagactgg
     8581 atggcaaggg gaagccgcag ctgtatctgc agacaccggc gcgagaaatc ttcgccgcat
     8641 tctcacccga cggccggtgg ctcgcctatg tgaatgatgc cgccggccgg tacgaggttt
     8701 acgtcgtcgc ggcggacgga acgggcgaca ggcggctggt gtccacggaa ggcggcgaag
     8761 aacctcgttg gtcggcgcgt ggggatctct tctaccgcaa cggtcagcaa tggatggccg
     8821 tcgccgtcgc gcccggcgcc gcat
//
LOCUS       DBNGSH010000621         8615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_742240, whole genome shotgun sequence.
ACCESSION   DBNGSH010000621 DBNGSH010000000
VERSION     DBNGSH010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_742240"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="AC16CY_14340"
     CDS             complement(<1..996)
                     /locus_tag="AC16CY_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIR3355025.1"
                     /translation="MSQEQQWYMAIGGHQVGPVSQDDVVTNLRNGTIDGNTLVFGPGL
                     SNWTPLKDVPPLASFLSGSSPRAAAPPPIVPGRRAHEIDFKIVGSEMQFVEVELDPGE
                     SAVAEAGSMMYMTPGISMETVFGDGAQQRQGVMGALLGAGKRLITGESLFMTVFTNSG
                     GGKQQVSFAAPYPGKILAMDLKQLGGQLICQKDSFLCAARGVSIGIAFQRRIGVGLFG
                     GEGFIMQKLEGDGLTFCHAGGTVHPVDLAAGQTLRVDTGCLVALQPTVSYDIEFVGKV
                     KTAFFGGEGLFFATLTGPGRAWLQSLPLSRMADRIYKAAPAAGGSRRGEGSILGGL"
     gene            complement(1029..1685)
                     /locus_tag="AC16CY_14345"
     CDS             complement(1029..1685)
                     /locus_tag="AC16CY_14345"
                     /inference="COORDINATES: protein motif:HMM:NF016291.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HIR3355026.1"
                     /translation="MDESMLINGVLRSVLGGRRRRSTRALDFLTGQSGSLWANPNVLL
                     TAAGLAWGVFETIQQQQGGTTPDMSGASNPGGASLPPLPAAAHTNEHVSDQALRVVRL
                     AISAANADGTISAEERQAIVAQARSANVDRLIEPELARPRPLDEIVAGVSDDGQRAAL
                     YTLAFGILRADEQPSGAERIYLAKLAHLLGLAPATVQQLEQNAGQRIDAQNQRVAGGG
                     "
     gene            complement(1739..3559)
                     /locus_tag="AC16CY_14350"
     CDS             complement(1739..3559)
                     /locus_tag="AC16CY_14350"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355027.1"
                     /translation="MSASAAWREARALLWAHRRRLALGLTLMLVSRIAGLVLPAAPKF
                     LIDDVILKGRSDLFPLIAIAAGVATLIQAATSFALSQILGVAAQRAITDMRKRVQARV
                     MHLPVRYFDSTQTGVLVSRIMTDAEGIRNLVGTGLVQLAGGLVTAVMALTILFWLNWH
                     LTTITILALAAFGGGMAYAFRTLRPLFRERGKINAEVTGRLTEALGGIRVVKSYTAEK
                     REEIVFTKGAHRLFRNIAKAMTGVSATTAGSTVIVGIVGVLMIWLGGNATMTGEMTLG
                     ELIMYVLFIGLVAAPLVSIASIGTQITEAFAGLDRIREILDMPTEVEEDEQRAPVETV
                     TGDVVFDDVWFEYNPGQPVLRGVSFRAAPGTTTALVGSSGSGKSTLISIVMAFNRPTK
                     GRVIVDGRDLSGLRLMDYRGQLASVLQENFLFDGTIADNVGYARPGASRAEIVEACRI
                     AHCDEFINQFPQGYDTVVGERGIKLSGGQRQRVSIARAILSDPRILILDEATSSLDSE
                     SEQMIQDGLRQLRSGRTTFVIAHRLSTIRSADQILVIEEGRVVEQGTHEELLALGGRY
                     RQLYDKQYRLETDRFINPGEDFTPEPERPAPQPARISQSL"
     gene            3716..4129
                     /gene="apaG"
                     /locus_tag="AC16CY_14355"
     CDS             3716..4129
                     /gene="apaG"
                     /locus_tag="AC16CY_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR3355028.1"
                     /translation="MNDISPQGIRTSDATTRGIRIQVRSEFIPDRSSPREGSYLFQYH
                     VRISNSGPDVTQLVSREWVITDADGEVARVKGQGVVGEQPVLEPGGWFEYTSLCPLKT
                     PVGTMQGTYQMITADGERFDAVIAPFTLAMPHALN"
     gene            complement(4139..4492)
                     /locus_tag="AC16CY_14360"
     CDS             complement(4139..4492)
                     /locus_tag="AC16CY_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3355029.1"
                     /translation="MQIGQRMAGDVAIVEVIGDITLSKGGDVVLKDKVQSLLQQGYRK
                     VLLDLGNVSYVDSAGLGQLVQIYATTNHLGGSMKLLNLTKRLKDLLVLTKLLTVFESY
                     DSEADAIASFDKSKK"
     gene            complement(4561..6168)
                     /locus_tag="AC16CY_14365"
     CDS             complement(4561..6168)
                     /locus_tag="AC16CY_14365"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3355030.1"
                     /translation="MSSEARLHVTDSQGRRVVALDRPQFLIGRRTSADLQIVSTDVSR
                     EHAEIVQEDGRFLLRDRQSRYGTFVNGQQVNEHPLQHGDRIRLGRTDAIELVFMTDGA
                     KTTGFLDAAASDASDLRQMAAILNGLRALGSGRVLDEVLGLVMDSALDVTKAERGFMM
                     LANDEGELEFKIARGKGRTTLPGTSFTTSEKIPREVFRTGESRMVGDLMEGDWKGEHD
                     GTIAVGIRHVLCVPLKVTPMSAAAASVHKVIGVLYLDGRERSTMLSQATRSSLEAFAT
                     QAALAIDSARLYAESAEKARIDRDLRIAADIQRALLPEPTFESASVDLAAATIPCRTV
                     GGDFYDYIEVGERGLGFALGDVAGKGPPAALLAAAVQTNFAAQAPVSADPAETMERVN
                     KALLRRAIEARFATMFYGAVTTAGHLSYCNAGQEPPLVVRRNEMSWLEVGGPVLGLLA
                     IATYEFESVALEPGDLVVVCSDGVTEARNQAGDEFGRDRLVETLRGRHGARPDAVLEH
                     LLGAVKQFSEGAAQADDVTALILRYRG"
     gene            complement(6173..6595)
                     /locus_tag="AC16CY_14370"
     CDS             complement(6173..6595)
                     /locus_tag="AC16CY_14370"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3355031.1"
                     /translation="MTREQMTAFFARRLEAINRHDAAELARHHTEDGMVDSPLSGASE
                     GREAIEKSYASSIRVFPDVKMTQELLLIDGHRVAWVVTMSGSGHAGLMGLPPAVRAFS
                     VPIVFLSEFENGLIARERRIYDFTGLLIQIGVLKAKPV"
     gene            complement(6684..8483)
                     /locus_tag="AC16CY_14375"
     CDS             complement(6684..8483)
                     /locus_tag="AC16CY_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3355032.1"
                     /translation="MSFPDLRSYIDQLRRDNDLVTVDVPVDAHLEVAEIHRRVVAAGG
                     PALLFTNVTGSDFRLVTNLFGTARRAELAFGDRPLRLIRRLVELVETLMPPTAAKIWD
                     ARDVGRALLSVGTRRVRSAPVVERVTGEVRLDRLPALTCWPEDGGPFITLPLVYTTHP
                     DRPGHNLGVYRMQVYDARTAGMHWQIGKGGGYHYAVAEGRGQSLPATVFLGGPPALIL
                     AAVAPLPENVPELMLASLIAGGRLGQVHGYGAHPLVADAEFALIGAVPPHRRRVEGPF
                     GDHYGYYSLAHEYPIFEVERVAHRADAIYPATVVGKPRQEDFFIGDLLQVLLSPLFPV
                     VMPAVERLWSYGETGYHSLAAAVVKQRYAREAMASAFRILGEGQLSLTKFLLVTDRHV
                     DLQDFRATLEHVLARTRPETDLYVFSNLSMDTLDYTGPKVNEGSKGVWMGLGDPVRDL
                     PRTFTAAELPAGVTDVHVFCGGCLVVGGPAYQSDPEASARIASAPAFAGWPLVVVSDE
                     AARATRSVMNFLWTTFTRFEPAADIHAAGRRIVRNHIAYSGPIVIDARLKPWFPKELS
                     CRDDIARTVTRRWREYFPTAAVDMGDSERAHLD"
BASE COUNT         1429 a         2988 c         2783 g         1415 t
ORIGIN      
        1 gaggccgccc agaatcgagc cctcgccgcg ccgcgacccg ccagcggcag gcgccgcctt
       61 gtagatgcga tccgccatgc gcgacagcgg cagcgactgc agccaggcgc ggccgggccc
      121 ggtcagcgtc gcgaagaaca gtccttcccc gccgaaaaac gccgtcttca ccttgccgac
      181 gaactcgatg tcgtagctga ccgtgggctg gagcgcgacg agacagcccg tgtccacccg
      241 cagcgtctgg ccggccgcga gatccaccgg atgcacggtg ccgccggcat ggcagaacgt
      301 gaggccgtcg ccttcgagct tctgcatgat gaagccctcg ccgccgaaca ggcccacgcc
      361 gatccgcctc tggaatgcga taccgatcga cacccccctg gccgcgcaga ggaacgagtc
      421 cttctggcaa atcagctgcc ctccgagctg cttgagatcc attgccagga tcttgcccgg
      481 gtacggcgcc gcgaacgaga cctgctgctt gcctccaccg gagttcgtga agaccgtcat
      541 gaagagactt tcacccgtga tcaagcgctt gcctgctcca agcagcgcgc ccatcacgcc
      601 ctgccgctgc tgggcgccgt ccccgaacac cgtctccatc gagatgcctg gagtcatgta
      661 catcatcgac ccggcctccg cgacggcgct ctcgccgggg tcgagctcga cttcgacgaa
      721 ctgcatctcg ctgcccacga tcttgaagtc gatctcgtgc gcgcggcggc ccgggacgat
      781 cggcggcggc gcggccgcgc gggggctcga gccgctcagg aacgacgcca gcggtggaac
      841 atccttgagg ggcgtccagt tcgacaggcc cggcccgaag acgagcgtgt tcccatcgat
      901 ggtgccgttc cgcaggttcg tcaccacatc gtcctgggat accggcccca cctgatgccc
      961 accgatggcc atgtaccact gctgttcctg tgacatctgc ctcttgtctc tctctctcgg
     1021 gctaccggct acccgccgcc ggctacccgc tggttctgcg cgtcgatcct ctggcccgcg
     1081 ttctgttcga gttgctgcac cgtcgcgggg gccaggccaa gcaggtgcgc gagtttcgcc
     1141 agatagatac gttcggcgcc gctcggctgt tcgtccgcgc gcagaatgcc gaacgccagc
     1201 gtgtacagcg ccgcgcgctg accatcgtcg ctgacgccgg caacgatttc gtcaagaggt
     1261 cttggccgcg ccagttccgg ttcgatgagc cgatcgacgt tcgcgctcct tgcctgagcg
     1321 acgatcgcct ggcgctcctc ggcgctgatc gtaccgtccg cgttagccgc cgaaattgcc
     1381 agccgcacga cgcgcagagc ctggtccgac acgtgctcgt tcgtgtgtgc cgctgccggc
     1441 agcggcggca gggaggcgcc gccaggattg gacgcgccag acatatccgg tgtcgtcccg
     1501 ccttgctgct gctgaatcgt ctcgaagacc ccccacgcca ggcctgccgc cgtcagcagt
     1561 acgttcgggt tcgcccaaag cgacccggat tgccccgtca ggaaatcaag tgcccgcgtc
     1621 gagcgccgcc gacgcccacc gaggacgctg cgaagcacgc cgttgatcaa catcgattcg
     1681 tccatcagtc tcctgtcccc tgccccctat cccccgcccc ctgccccctg ccccctgact
     1741 agaggctttg cgatatgcga gcgggctgcg gtgcgggccg ctccggctcg ggtgtgaagt
     1801 cctcgcccgg attgatgaag cggtccgtct ccaacctgta ctgcttgtcg tacagctgcc
     1861 tgtaccggcc tccgagcgcg agcagctcct cgtgcgttcc ctgttcgacg acacggccct
     1921 cctcgatcac caggatctgg tccgcgctgc ggatggtgga caaccggtgg gcgatgacga
     1981 acgtcgtgcg tccggaccgc agttgtcgca ggccgtcctg aatcatctgc tcgctttcgc
     2041 tgtcgaggct cgacgttgcc tcgtcgagaa tcaggatgcg cggatcggac aggatcgccc
     2101 gagcgatcga gacccgctga cgctggcctc cggagagctt gatgccccgt tcgccgacca
     2161 cggtgtcgta gccctgcgga aactgattga tgaactcgtc gcaatgggcg atgcggcagg
     2221 cctcgacgat ctcggccctc gaggcgccgg gcctggcata cccgacgttg tcggcgatcg
     2281 tgccgtcgaa caggaagttc tcctggagta ccgacgcgag ctggccccga tagtccatca
     2341 gccgcaggcc ggacaggtcc cgtccgtcca cgatcacgcg ccctttcgtc ggccggttga
     2401 acgccatgac gatgctgatg agagtgctct tgccggagcc gctcgagccg accagggccg
     2461 tggtcgtacc gggcgccgcg cggaacgaca cgccgcgaag caccggctgc cccgggttgt
     2521 attcgaacca gacgtcgtcg aagaccacgt cgcccgtcac tgtctcgacg ggcgcgcgct
     2581 gttcgtcctc ttcgacctcg gttggcatgt cgaggatctc gcgaatgcga tccagcccgg
     2641 cgaaggcctc cgtgatctgg gtgccgatcg acgcgatcga cacgagcggg gccgcgacga
     2701 gcccgatgaa caggacgtac atgatcagct caccgagcgt catctccccg gtcatggtcg
     2761 cgttcccgcc gagccagatc atcaacacgc ctacgatgcc gacgatcacg gtgctgcccg
     2821 ccgtggtcgc cgagacgccg gtcatcgcct tcgcgatgtt gcgaaagagc cggtgggccc
     2881 ccttcgtgaa cacgatctct tcgcgcttct ccgcggtgta ggacttcacg acgcgaatgc
     2941 cgccgagggc ctcggtcagc cggccggtga cctccgcgtt gatcttcccg cgctcgcgga
     3001 acagcggccg cagcgtgcgg aaggcgtacg ccatgccgcc gccgaacgcc gccagggcga
     3061 ggatcgtgat cgtggtcagg tgccagttca gccagaacag gatggtgagc gccatcaccg
     3121 ccgtcaccag cccgcccgcg agctgcacga ggccggtccc gacgagattc cggatccctt
     3181 cggcgtcggt catgatccgc gagacgagca cgccggtctg cgtggaatcg aagtagcgga
     3241 cggggagatg catcacgcgc gcctggactc tcttgcgcat gtcggtgatc gcgcgctgcg
     3301 ccgccacgcc gagaatctgt gagagcgcga acgacgttgc ggcttgaatc agcgtcgcca
     3361 cgccggccgc aatcgcgatc agcggaaaca ggtccgagcg tcccttcagg atgacgtcat
     3421 cgatcaggaa cttgggagcg gcggggagca cgaggcccgc gatccggctg acgagcatca
     3481 gcgtcagtcc gagcgcgagg cggcggcggt gtgcccagag tagcgcgcgc gcctcgcgcc
     3541 acgcggcaga cgcggacacg cctttcttct tcggcggttc catcgacggc actgaaactg
     3601 tactacggcg aaggcgctcg ccggttccag gcccgcccgg cctcaacagc gcggccatga
     3661 cataatcaac actgactccc gaccgaaacc tcgcgtctca ggtctttttg tgcaaatgaa
     3721 cgatatcagc ccgcagggaa tccgcacgag cgacgccaca acaagaggaa ttcgaatcca
     3781 ggtccgcagc gagttcattc ccgaccgcag ctctccccgc gaaggatcct atctgttcca
     3841 gtatcacgtc cgcatctcca actcgggccc cgatgtcact cagctcgtgt cgcgcgaatg
     3901 ggtcatcacc gacgccgacg gggaagttgc gcgcgtcaag ggtcaggggg tcgtcggcga
     3961 gcagccggtg ctggagcccg gcgggtggtt cgagtacacg agcctgtgtc cgctgaagac
     4021 gccggtcgga acgatgcagg gcacctacca gatgatcacg gccgacggcg aacgcttcga
     4081 cgccgtcatc gcgccgttca ccctcgccat gcctcacgcg ctcaattgaa ccgccgtcct
     4141 acttcttgct cttgtcgaaa ctggcgatgg cgtcggcctc ggagtcgtag ctctcgaaca
     4201 ccgtcagcag cttggtcagc accagcaggt ccttcagccg cttcgtcagg ttgagcagct
     4261 tcatcgaccc acccagatgg ttcgtcgtgg cgtagatctg gacgagttgg ccgagaccgg
     4321 cgctgtcgac gtaggacacg ttgccgaggt cgagcagcac tttccgatac ccctgctgca
     4381 ggaggctctg caccttgtcc ttcaggacga cgtctccacc cttgctcaac gtgatgtcgc
     4441 cgatgacctc gacgatggcc acgtcccccg ccattcgctg tccgatctgc attcgtcctc
     4501 cctgcgtgcc ccgcgcgtgc agcagggtgc atgttactcc agttgattcc cgtcctccgc
     4561 ctagccgcgg taccggagaa tgagcgctgt gacgtcgtcg gcctgcgccg caccttcgga
     4621 gaactgcttg accgctccga gcaggtgctc gagcacggcg tcaggcctgg ctccgtggcg
     4681 gccgcgcagc gtctccacga ggcggtcacg cccgaactcg tcgccggcct ggtttcgcgc
     4741 ctcggtcacg ccgtcgctgc acaccacgac gagatcgccc ggttcgagcg ccaccgattc
     4801 gaactcgtaa gtcgcaatgg ccagcagtcc gaggaccggg ccgcccacct cgagccacga
     4861 catctcgttc ctgcgtacga cgaggggcgg ctcctgaccc gcgttgcagt agctcagatg
     4921 tccggccgta gtgaccgcgc catagaacat cgtcgcgaac cgcgcctcga tcgcccggcg
     4981 cagcagcgcc ttgttcaccc gctccatcgt ctcggcagga tccgcgctca ccggggcctg
     5041 cgcggcgaag ttggtctgga cggcggcagc caggagcgca gcgggtggtc ccttcccggc
     5101 gacgtcgccg agcgcgaagc cgagcccccg ctcccccacc tcgatgtaat cgtagaagtc
     5161 gcccccgacc gtgcggcagg gaatggtggc ggccgccagg tcgaccgacg cgctctcgaa
     5221 ggtcggctcc ggcaggagcg cgcgctggat gtccgccgca atccgcaggt cacggtcgat
     5281 acgagccttc tcggccgact cggcgtagag gcgcgcgctg tcgatggcca gcgccgcctg
     5341 cgtggcgaag gcttcgaggg atgaccgggt ggcctgcgac agcatcgtgc tgcgctcgcg
     5401 gccatccaga tacaagacgc cgatgacctt atgaacggac gcggccgccg ccgacatcgg
     5461 cgtcaccttc agcggcacgc agaggacgtg acggatgccc accgcgatcg tcccgtcgtg
     5521 ctctcccttc cagtcgccct ccatgagatc gccgaccatc cggctctcgc cggtgcggaa
     5581 gacctcgcgc ggaatctttt cgctggtcgt aaaggacgtg cccggaagcg tggtgcgccc
     5641 tttgcccctg gcgatcttga actcgagctc gccttcgtca ttggcgagca tcatgaagcc
     5701 gcgctccgcc ttggtgacgt cgagcgccga gtccatcacc aggccgagga cttcgtcgag
     5761 gacgcggccg gagccgagcg cgcgcaggcc gttcagaatc gccgccatct gacgaaggtc
     5821 gctcgcatcg gaggcggcgg cgtcgaggaa ccccgttgtc ttggccccgt cggtcatgaa
     5881 cacgagctcg atcgcgtcgg tccgccccag gcggatgcgg tcgccgtgct ggagcgggtg
     5941 ttcgttgacc tgctggccgt tcacgaacgt cccgtagcgg gattggcggt cgcgaagcag
     6001 gaagcggccg tcctcctgaa cgatctccgc gtgctcacgc gatacgtcgg tgctgacaat
     6061 ctgcaggtcg gcggaggtcc gccgtccgat gagaaactgc ggccggtcga gcgcgacgac
     6121 gcggcggcct tgagagtcgg tgacgtgaag ccgggcttcg ctggacatgg gactagaccg
     6181 gcttcgcctt gaggaccccg atctgtatca gcagcccggt gaaatcgtag atgcggcgtt
     6241 cccgggcgat caggccgttc tcgaactcgc tgagaaacac gattgggaca ctgaacgccc
     6301 gtaccgcggg aggaaggccc atgaggccgg cgtggccgga acccgacatg gtcacgaccc
     6361 aggcgacgcg atgaccgtca atcaacagca actcctgggt catcttcacg tcgggaaaca
     6421 cgcggatgct cgacgcgtag gatttctcaa tcgcctcgcg gccctcggag gcgccggaaa
     6481 gcgggctgtc gaccatgccg tcttcggtat gatgccgcgc gagctctgcc gcgtcgtggc
     6541 ggtttatcgc ctcgagtcgc cgggcaaaga acgccgtcat ctgctctcgc gtcatcagtg
     6601 cgcccgttcg ctcacaccag cctacagagc ttaggtggct ggtgcacgtg gagtcaacgc
     6661 atcggcgagg tccgacacag gcatcaatcc aggtgcgcgc gctccgagtc gcccatgtcc
     6721 accgcggcgg tggggaaata ctcccgccaa cggcgggtca ccgtgcgcgc tatgtcgtcg
     6781 cggcaggaca gttcctttgg aaaccacggc ttcagacgag cgtcgatgac aatcggcccg
     6841 ctgtaagcga tgtggttgcg gacgatccgg cgcccggccg catgaatgtc ggcggctggc
     6901 tcaaaccgtg taaaggtcgt ccacaggaag ttcatgacgc tccgggtcgc gcgcgcggcc
     6961 tcatcactga caacgacgag cggccatcca gcgaacgccg gggcggacgc gatccgcgcg
     7021 gacgcctccg gatcggactg gtacgcgggc ccaccgacca cgaggcagcc gccgcagaag
     7081 acgtggacgt cggtgacgcc ggccggaagc tcggcggccg tgaaggtccg cggcaggtcg
     7141 cgcaccggat cgcccagccc catccacacg cccttcgacc cctcgttgac cttcggcccg
     7201 gtgtagtcga gcgtgtccat cgagaggttc gaaaacacgt agagatcggt ctcgggccgc
     7261 gtgcgcgcga gcacgtgttc gagcgtggcc cggaaatcct ggaggtccac gtggcggtcc
     7321 gtcaccagca ggaacttggt cagcgacagc tgcccttcgc cgagaatccg gaacgcgctg
     7381 gccatcgcct cgcgcgcgta gcgctgcttg acgacggccg ccgcaagcga gtggtagccc
     7441 gtttccccat acgaccagag acgctcgacc gcgggcatga cgacgggaaa gagcggcgag
     7501 aggagcacct gcagcagatc gccgatgaag aaatcctcct ggcgcggctt cccgaccacg
     7561 gtcgcggggt agatggcgtc ggcacgatgc gccacgcgct cgacctcgaa gatcgggtac
     7621 tcgtgcgcca gcgagtaata gccgtagtga tcgccgaagg gcccctcgac gcgccgccgg
     7681 tgcggaggaa ccgctccgat caaggcgaac tcggcgtcgg cgacgagcgg atgcgcgccg
     7741 tatccgtgaa cctggccgag ccggccgccg gcgatgaggg aggcgagcat cagctccgga
     7801 acgttctccg gcagcggcgc gacggccgcg aggatcagcg ccggcgggcc gcccaagaac
     7861 acggtcgccg gcaggctctg tccccggccc tcggcgaccg cgtagtgata gcctccgccc
     7921 ttgccgattt gccagtgcat accggccgtt cgtgcgtcgt acacctgcat ccggtacacg
     7981 ccgaggttat gaccgggacg atccggatgc gtcgtgtaga cgagcggcag cgtgatgaac
     8041 gggccgccgt cctcgggcca gcacgtgagc gccggcaggc ggtcgagccg cacctcgccg
     8101 gtcacacgct cgacgactgg cgcggatcgg acccgcctgg tccccacgct gagcagcgcg
     8161 cggccaacgt cccgcgcgtc ccagatcttg gcggcggtcg ggggcatcag cgtctcgacg
     8221 agctccacca ggcgccggat caggcgaagc ggccggtcgc cgaaggcgag ctccgcccgc
     8281 ctggctgtgc caaacaggtt ggtgacgagg cggaaatccg accccgtgac gttggtgaac
     8341 agcagcgcgg gtccgccggc ggccacgacc cgccggtgaa tctcggcaac ctcgaggtgc
     8401 gcgtcaaccg gaacatccac cgtgacaagg tcgttgtcgc ggcgaagctg gtcaatgtag
     8461 gagcgaagat cgggaaacga cactactgct actgccggcc gcctaccgcg ggctgccccc
     8521 cgccgccggc ctactccttg tcatcggcgc tcgggccgta gatgttcgga accctcgatc
     8581 ccatcgcccg caagtacgcc gcgagctggc cgcga
//
LOCUS       DBNGSH010000622         3484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_572722, whole genome shotgun sequence.
ACCESSION   DBNGSH010000622 DBNGSH010000000
VERSION     DBNGSH010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_572722"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3431)
                     /locus_tag="AC16CY_14380"
     CDS             complement(<1..3431)
                     /locus_tag="AC16CY_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIR3355033.1"
                     /translation="MLLTGRLDRERLRQSVARVVGRHEILRTTFRQPSGLKLPIQVVG
                     DETEPVWRETQVSVGEAALPDVVETLLRRDREPLSVADGSPLRCTLARVDGSQHVLLM
                     TASPLVADLESLYLAANDAMRIYASGGRDAAGDEPLQYAAFAAWQAEILEDEEATLGR
                     KYWLSQDVAALTGIQHPLVRPPADAGDVRVERVEREIDPSLVNAIDDLARRAAKTDQQ
                     VYLSCWCALLGRLTGSTDIPVACRIDGRTDQELETAVGPFAGFVPLRYRATNGASFQT
                     AIDNIVDSRERARDWEPYFDWEDVAADSGSGEPLFLPFAFEYRRVPAESEASGIRISP
                     LREFEYLDRFHLKFACSRRGNSVVLELHYAAAMFSREDASRLAGQLETALRGVVHDPT
                     RDCAEIDILGAEEWTRTIEDFNATAVAFPDDPCAHDLFEAQVARCPDRCAVVYEDGVL
                     TYRALDDAATALAARLRVRGVGAGRTVGIHAERSADMIVAILGILKAGGAYVPLDQSL
                     PKLRLEALVRESHMTSVVSHRALAGVFQDIDIDVVVLDDVLRSQGAAPEPSAVMVTPA
                     DLAYVLFTSGTTGVPKGVAVEHRQIVNYARGVAERFEVCDGEHMALVSTFAADLGNTV
                     VYAALTTGGTLHVVSEHRRSNPDALAQYFARWPIDCIKVVPSHLEALLSASAPQHVLS
                     CRRIVLGGEATPWALIDRIEALSSAKVFNHYGPTETTVGATTCALSRGRRDPDSGSLP
                     IGRPLPNYRAYILGSGGRPAPIGVTGELYIGGGGVARGYLNRPAETAERFVPDPIGAD
                     GQRLYRTGDRARQLPGGDIEFRGRVDDQIKIRGYRVEQAEVEAVVAACPAVRHAVVVA
                     RREAAGEARLVAYVVLRQAEATVADLTAFAEQRLPDYMVPSVFVTLPSLPLNRNGKVD
                     RRALPAPDVSTARENLVEPRTATEQRLADIWRQLLRLDVIGVRDSFFDRGGHSLSAMQ
                     LVSRIRQQFDVELPLLSIFESPTIEGIAARIEEASEQPARASLPPIVATRRDRVIPAS
                     FSQERLWLFDQLQPGNSAYNVPAAVQIGGALDVAALEQTIAAVVSRHEVLRTTFSSVD
                     GRPVQVIAPDSRARISFTDLRTLATPERARLVEAIARSHAQRSFDLAKGPVWRASL"
BASE COUNT          610 a         1184 c         1131 g          559 t
ORIGIN      
        1 agcgacgctc gccagacagg acccttcgcg agatcaaacg agcgctgggc gtgggacctg
       61 gctatcgcct caacgagacg ggctcgctcc ggtgtcgcga gtgtccgcag gtcggtgaac
      121 gaaatccggg cccggctgtc gggggcgatg acctgcaccg gtcgcccatc gactgaactg
      181 aacgtggtcc gcaacacctc gtgccgactg acgactgcgg caatggtctg ttccagcgct
      241 gcgacgtcga gcgcaccgcc aatctgcacg gccgcgggca cgttgtacgc gctgttgccg
      301 ggctgcagct gatcgaagag ccacagtcgc tcctgcgaga acgacgccgg aatgacgcga
      361 tcccgccgcg tggcgacgat cggtggcaag ctggctctcg ccggctgctc cgacgcctcc
      421 tcgatccttg cggcgatgcc ctcgatggtc gggctctcga aaatactgag cagcggcagc
      481 tcgacgtcga actgctggcg gattcgtgag acgagctgca tcgccgacag cgagtgcccg
      541 cctcggtcga agaagctatc tcgtacgccg atgacgtcga ggcgcaggag ctggcgccag
      601 atgtcggcga gtctctgctc ggtggccgtg cgcggctcca ccaggttctc ccgcgcagtc
      661 gacacgtcag gagccggcag cgcccgccga tccaccttgc cgttgcgatt gaggggaagg
      721 ctcggcagcg tgacgaatac cgatggcacc atgtagtcgg gcagacgctg ctccgcgaat
      781 gcggtgagat ccgcgacagt cgcctcggcc tgccgcagga caacgtaggc gacgaggcga
      841 gcctcgcctg ccgcttcccg ccgtgccacg acgaccgcgt gccgaacggc cggacaggca
      901 gccacgaccg cctcgacctc cgcttgttcc acgcggtacc cgcggatctt gatctggtcg
      961 tcgacgcgcc cgcggaactc gatgtcgccg cctggaagct ggcgtgcccg atcgcccgtc
     1021 cgatagagcc gctgcccgtc ggcgccaatc gggtctggta cgaaccgttc ggctgtctcg
     1081 gctggccgat tgaggtaccc gcgcgcgaca ccgccaccgc cgatgtacag ctcgccagtg
     1141 actccgatcg gcgcgggtcg tccaccggac cccaggatgt aagcgcgata gttgggtagc
     1201 ggccgtccga tcggcaggct tccgctgtcg gggtcgcgtc ggcccctcga gagcgcgcag
     1261 gtcgtggccc cgacggtcgt ctcggtcggc ccgtagtgat tgaagacctt ggccgacgac
     1321 agcgcctcga tgcgatcgat cagcgcccac ggcgtggcct cgccgccgag gacgatgcgg
     1381 cgacaggaaa gcacatgctg aggagcggac gccgacagaa gtgcctccag gtgcgacggg
     1441 accaccttga tgcagtcgat cggccagcgg gcgaagtact gggcgagcgc gtccggattc
     1501 gaccggcggt gctcagacac gacgtgaagc gtcccgccag tagtcagggc ggcgtacacg
     1561 accgtgtttc ccaggtctgc cgcgaacgtt gagaccaggg ccatgtgctc gccgtcgcag
     1621 acctcgaatc gttccgcaac accccttgcg tagttcacga tctgtcgatg ttcgactgcc
     1681 actcccttcg gcacgcctgt cgtgccggac gtgaacagca cgtacgcgag gtcggctggc
     1741 gtcaccatca cggcgctcgg ctctggcgct gcgccctggg accgcaggac gtcgtcgagg
     1801 acgacaacgt cgatgtcaat gtcctggaat acgccggcga gggcacggtg cgacacgacg
     1861 cttgtcatgt gcgattcgcg cacgagtgct tccaggcgca acttgggcaa cgactggtcg
     1921 agagggacgt aggcgccgcc agccttcagg atcccgagga tcgcgacgat catgtccgcg
     1981 gaccgctcgg cgtgtatgcc gaccgtcctc ccggcaccaa cccccctgac ccggagacga
     2041 gccgcaagcg cagtcgccgc atcatcaagt gcgcgatagg tcaggacgcc gtcctcgtag
     2101 acgaccgcgc accggtctgg acagcgcgca acctgcgctt cgaacagatc gtgggcgcac
     2161 gggtcatcgg ggaacgcgac ggctgtcgca ttgaaatcct cgatcgttcg cgtccattcc
     2221 tccgcgccga ggatgtcgat ttcggcgcaa tcccgcgtcg ggtcgtgtac cacgccgcgg
     2281 agtgccgtct cgagctgacc ggccaggcgc gacgcatcct ctcgggaaaa catggcagcc
     2341 gcgtagtgca actccaggac gaccgagttg ccgcgacggg aacacgcgaa cttcaagtgg
     2401 aagcggtcca ggtattcgaa ctcgcgaagt ggcgagattc ggatgccgga cgcttcgctc
     2461 tcggccggaa cgcgccggta ttcgaaggcg aatggcagaa acagcggctc gccgctcccc
     2521 gaatcggcag ccacgtcctc ccagtcgaag tacggctccc agtcccgtgc gcgctcgcgg
     2581 ctgtcgacga tgttgtcaat ggccgtctga aacgaagctc cgttcgtggc ccggtaccgg
     2641 aggggcacga acccggcaaa cggaccgacc gccgtctcca gttcctggtc ggtgcggccg
     2701 tcgatccgac aggccaccgg aatgtcagtg ctgcccgtca accgtccgag cagtgcgcac
     2761 cagcaggaga gatacacttg ctgatcggtc ttggccgcac gccgcgcaag gtcatcgatg
     2821 gcgttgacca ggctcggatc gatctcacgc tcgacgcgct cgacgcgcac atcccccgca
     2881 tccgccggag gtctgaccag cggatgctgg atcccggtca gcgcggcaac gtcctgcgac
     2941 agccaatact tccggccgag cgttgcctcc tcgtcctcga ggatctcggc ctgccacgca
     3001 gcgaaggccg cgtactgcag cggttcatcg ccggcggcgt cacgaccccc actggcgtag
     3061 attcgcatcg cgtcgttggc ggccaggtac aacgactcga gatcggcgac caggggcgaa
     3121 gctgtcatga gaaggacatg ctgcgacccg tcaaccctgg caagcgtgca gcgcaaagga
     3181 ctgccgtcgg cgacgctcag cggctcgcgg tctctccgca gaagcgtctc aacgacgtcg
     3241 ggcagtgcgg cctctccgac tgaaacctga gtctcccgcc agactggctc cgtctcgtca
     3301 cctacgacct gaatcggcag cttcagcccg ctgggctgcc gaaacgtcgt ccgcagaatt
     3361 tcgtgacgcc ccactacgcg cgcgaccgac tgcctgagcc gctctcgatc gagtcgacca
     3421 gtgagcaaca acgcagcttg aaccgcaaat gcggtcccgt agtgaggaaa ctgccagatc
     3481 cgtt
//
LOCUS       DBNGSH010000623         9802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1591212, whole genome shotgun sequence.
ACCESSION   DBNGSH010000623 DBNGSH010000000
VERSION     DBNGSH010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1591212"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="AC16CY_14385"
     CDS             <1..911
                     /locus_tag="AC16CY_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355034.1"
                     /translation="EPRIVLFEAKPAAVRAGQPVLLVWHVENPTGISIEPGIGAVTPR
                     GSRQVTPSATTTYILSVKGAPPRSLVVTVEGPAPASATPAPAASTPAGVRRMPDGKPD
                     FSGVYGNAGLPQGTTPPPLKPGAEKYRIVRGGPRDVKGRTTITTGNDCYPLGVPQTYI
                     TPYPFQIVHLPKLVIIIYEYPNAVRFVPIDGRPQVSDPDPSWMGTSVGRWDGDTLVID
                     AVGFNDKTEVHGFMHTDALHVVERFRRLENGSLQYEVTVEDPNVWETPWAIPARTFPF
                     RPELEFVAEFVCESTVDYGKLFKSEK"
     gene            978..1349
                     /locus_tag="AC16CY_14390"
     CDS             978..1349
                     /locus_tag="AC16CY_14390"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355035.1"
                     /translation="MKLRLTVLVAMFCVLMTTGPTSAHHSTAMYDSQNPVTITGAVKK
                     FEWTNPHAFVYLEVKGADGKVVEWEVEMMSLNHLRAYGWTSKTVKPGEIISATGAPAR
                     SGAPSMIANRMKLPDGREIRS"
     gene            1383..2471
                     /locus_tag="AC16CY_14395"
     CDS             1383..2471
                     /locus_tag="AC16CY_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355036.1"
                     /translation="MLRRLLFAVVGIALATATAWVQAPQEAPAAPGAGARGGGRGRAA
                     GPPPRIMTFEAHPASITLGESVLLVWSTENPAAPAIEPDLGPVLPRGSRRLTPGATTT
                     YTLTMRGPNDTVVTRSVTVTVAGTAFVSVGSAATPPASGPIPRTPDGKPDFSGVYAFG
                     GGGRGRGAGAPADGIARTPTLKPGAEQYRVARGSLDVGRTADCMPLSPPDAFGVPYQF
                     QIVQNKDLLVILHEYPGTFRLVPLDGEPHQVDPDPSWLGDSVGRWEGDVLVVDTIGYN
                     DKTQVSGFRHSEALHTVERFRRIEGGFEYDVTIEDPNVFVGPWTETRTYRLNVPPMKR
                     IMEFVCENNRDYRPLFGPQGAQPAQPGR"
     gene            complement(2492..2992)
                     /locus_tag="AC16CY_14400"
     CDS             complement(2492..2992)
                     /locus_tag="AC16CY_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355037.1"
                     /translation="MASNRASIVVALVLTVFVTPRATAHHSYAMYDGAVYRVFTGVIV
                     RVVPNAAHFEIHFVPLNDARDALVRDQKGEPFVWVVQMESAAQALKDGITREAFPQGT
                     VFSMGLHPRRDGKPAGDRGESGLFLCPKGSKPEPGKHCDSVAGSKSFGAGQLPKHTPA
                     TRPSGN"
     gene            complement(2979..4298)
                     /locus_tag="AC16CY_14405"
     CDS             complement(2979..4298)
                     /locus_tag="AC16CY_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355038.1"
                     /translation="MPAVRSTFEVAVFSLAVIAGATPTAFAQLRPPEVPGLRGAPFPS
                     MNPPPEVRSYGEGPQWDGRPPEGVQPLATDLFTSKDFYKDRDRWMDQRYWRCNAPRQV
                     ADMRSGGAGSSTNDPRIGSKPPGSARWGDCAMDWPRENIVSPYRFTTAKDHYEALRAD
                     ATSRGGPTRHTYETMPRWDGVYGEYAPNGRRVWNYSRANQVPTVLSLLTPEYQQRMVR
                     QLYHEGVNAAHQWSASYCWPEGFMRQWATGPKPGRIVVTPEVVLFMGSGSGNMWRVVH
                     LDRAFPLGQGVPQWYGDTIGFWDGDALITWTANVQGWNQHSSWEWSDRLETIEILTPV
                     RDSAGRFLGIDWEAVIYDADALVQPVRILSHRNYQQGWSTADRLGWAECTRPLYPIDG
                     YATPVAPGQVIQYKVPDMLDRPWAKIWEEYFEKNMDRPKKDLDLGFK"
     gene            4418..5764
                     /locus_tag="AC16CY_14410"
     CDS             4418..5764
                     /locus_tag="AC16CY_14410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3355039.1"
                     /translation="MLTRRSVVALVVLCLAALVRPALREVEPLALSGVEATLLAAVPS
                     ARPEDVGLSAERLKRIGELMQRHIAAKTFPGAVTLVARNGRIAHFDVHGLMDLETQKP
                     MQKDAIFRIMSMTKPVVGVAILMLVEEGKVRLADPASKFIPELQGLSAVVLNTDGRVS
                     PAPSGSVAAPLPGRTVPADREVTVRDLLTHTSGLMSGGASAASTNDVMPKAGETVAQV
                     MPRLKKVPLDFQPGTRWAYSPQFGFDVLLRVVEVASGMPFDRFARQRIFEPLGMKDTF
                     FYPTDGNSRIATLYEQANGVLRKAANPAFMNGAYVSGGGGLFSTAEDYLQFALMLLNR
                     GELDGKRLLSVRTVDLMATSFAPDTFPGRLAGEGFGLSVRVVDNPAARTTYLSKGSFG
                     WSGAFNTHFFIDPAERIVGLFMTQIVGLSTRGDVRDDFETAVMQSIVGSGAPSGSN"
     gene            5800..8277
                     /locus_tag="AC16CY_14415"
     CDS             5800..8277
                     /locus_tag="AC16CY_14415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355040.1"
                     /translation="MWRDLKSVLRTLIRQPLVATVVVGTVALGIGGSVAVFSVVNAVL
                     IRELPYADAGRLYVMRAVAPDGRLGNITHREFTPIYQNDQHAIVEKAAIVWSQDSQIV
                     GADKRPHLTVRYGVTDRFFDVFGTRMAIGSGFRPGDLPGRIVISYPIWRDAFGSDPNI
                     IGKRVVAEGMTLEVVGVTPADFDFPEDPGFWYLMRLGSGFDKVRGYRGFMRLREGSTP
                     EQIDAEMKRVATTLGQDPVTKQVPILVAQPLLEHVVGNDLKSTVMIVFGATGILLLIA
                     CINVTNLLLSRATVRSREVALREALGAGRWRILRPFLIESLVLTTVGGALGLAAAAAG
                     IRVFLAVAPTGLPRLDTVPIDGTVLIFAAGATLLTGVLVGLAPAVRLARNQLRSLINE
                     GGRGASGGPSQNRVFSVLVVTETALAVVLVVGAGLLVRSYYNLTTTDPGFNPDRVLTV
                     SMNVPGRMTASVVQNAQGKPEFRASYAPMASFFRELEQRLGGITGVSGVATTTLLPLD
                     PDSAGVSIDFTLPERTGATPDAAAWTSPLHSVSPEFFDTMGMRLLTGRNITRSDQQGS
                     PGAAVVNETFVRRFLSGGSPLGERIRWRLNRYVPTDTGFQFGHLTVEEVEVVGVVTDA
                     KYASLAKPAEPAIYVSSEQFIFRRRTVAVRAAGDNPESLVSPVRRELAAMDPLIGARF
                     AAYDSIVHDSIARQRLGMSLLVAFGLAALLLAAVGVYGLLSYSVAQRTGEIAVRSAMG
                     SSATQVMRLVMGKGMRLAVAGIVIGALGAVALRQVVAGQLYGVSPLDLSVFLLVIIVL
                     LGVSVLACVVPARRATRIDPVTLLKAE"
     gene            8364..9086
                     /locus_tag="AC16CY_14420"
     CDS             8364..9086
                     /locus_tag="AC16CY_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355041.1"
                     /translation="MRNLVLLAFLLGVPSVAFGQTADEIVAANITASGGEAAIARIQN
                     ITTKGRVTIESKFFGKLEGTLETVRVPGRGYYERAALGPIEQTKGWDGTRGWEQGPNG
                     SRTLDGFELDVMRVQSFVNPFVAQRTLAPPGLRIERLDDAPVDGRPHYVLIVKTSEGP
                     PATMYIDRETKLLTRSIMTVSIPNVGEATIVTDVGSYKPVAGVMMSTTLTQVAEGLAT
                     TTVILDDTAVNTAMDAKIFASR"
     gene            9099..9569
                     /locus_tag="AC16CY_14425"
     CDS             9099..9569
                     /locus_tag="AC16CY_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355042.1"
                     /translation="MDFEGAFLAWTALAIFIPLALSVAVPGVYRTWVEYRSRSQALDV
                     LRVYAEKGQEPPASVMGALAVAAAPTAPAAHASLPPSVFPGPTREKHLAHLAGSLVCG
                     LGATGVAWWRWPADGNPGPLVIWAVVIAIFFAGGVAARLVGVFTTPSGRLRDGR"
     gene            9559..>9802
                     /locus_tag="AC16CY_14430"
     CDS             9559..>9802
                     /locus_tag="AC16CY_14430"
                     /inference="COORDINATES: protein motif:HMM:NF016430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3355043.1"
                     /translation="MADDDVALIADIRNGSERAFNVLIDRHQQAVRDFLRRLLADASE
                     ADDLAQETFMVAWTRPGTYRGDASVRSWLCAIAWRKA"
BASE COUNT         1599 a         3200 c         3309 g         1694 t
ORIGIN      
        1 tcgaaccgcg gattgtgttg ttcgaggcga aacccgccgc cgtgcgagcg ggtcaaccgg
       61 tgctccttgt ctggcacgtc gagaacccga cgggcatctc catagaaccg gggatcggcg
      121 cggtcacgcc gcgtggcagc aggcaagtga cgccctcggc gaccaccacg tacatactct
      181 ctgtcaaagg cgcgccgccg cgcagcctag tggtcacggt cgaaggcccc gctcccgcga
      241 gcgcgactcc ggcgccggcc gcctctacac cggcgggcgt gcggcgaatg cctgacggca
      301 agcccgattt ctcgggcgtg tacggcaatg ctggcctccc tcagggaacg acgcctccgc
      361 ccctcaagcc gggagccgag aagtaccgga tcgtccgcgg cgggccgcgt gacgtgaagg
      421 gacggacaac gatcacgacg ggcaacgatt gctatccgct cggcgtgccg cagacctaca
      481 tcacgccgta ccccttccag atcgtccacc tgccgaagtt ggtgatcatc atctacgagt
      541 atccgaacgc cgtccggttc gttccgatcg acggacgccc gcaggtcagc gatccggatc
      601 cctcctggat ggggacctcc gtgggccgat gggatggcga cacgctggtc atcgacgcgg
      661 tcggcttcaa cgacaagacg gaagtgcacg gcttcatgca caccgacgcg ctgcacgtgg
      721 tggagcgctt ccgccgcctc gagaacggct cgctgcagta cgaggtcacc gtcgaagacc
      781 cgaacgtgtg ggaaacaccc tgggcgattc cggcgcggac attcccgttc cgccccgagc
      841 tggaattcgt cgcggagttc gtctgcgaga gcacggtgga ctacgggaag ctgttcaaga
      901 gcgagaagtg agcagggggc agggggcagg ggacaggggg caggggacag ggggcagggg
      961 accggggagg ctggacgatg aaattgcgat tgacggtgct cgtggcgatg ttctgcgtgc
     1021 tgatgacgac ggggccgacg agcgcgcacc attcgaccgc catgtacgac tcccagaatc
     1081 cggtgacgat cacgggcgcg gtcaagaagt tcgaatggac caatccgcac gccttcgtct
     1141 atctcgaggt caagggcgcc gacggcaagg tcgtcgaatg ggaagtcgag atgatgagcc
     1201 tgaaccacct cagggcctat gggtggacca gcaagaccgt caagcccgga gaaataatct
     1261 cggccacggg cgctccggcc cgcagcggcg cgccgtcgat gatcgccaac cgcatgaagc
     1321 tccccgacgg ccgcgagatc cggtcctgaa ggtccgcagg gaggacgatc cgggaggaca
     1381 ctgtgctgag gcgccttctg tttgccgtcg tcgggatagc gctggccacc gcgacagcgt
     1441 gggttcaggc gccgcaggag gcgccggccg cgccaggcgc cggcgctcga gggggaggcc
     1501 ggggacgcgc ggcgggaccg ccgccgcgca tcatgacgtt cgaagcgcat cccgcgtcga
     1561 tcacgctggg ggaatcggtc ctgctcgtgt ggagcaccga aaatcccgca gcgccggcca
     1621 tcgagcccga tctcggcccg gtgctgccgc ggggaagccg gcggctgacg cccggtgcca
     1681 cgacaaccta cacgctgacg atgcgcgggc ccaacgatac tgtggtgacc aggtcagtca
     1741 ccgtgacggt tgccggcacg gccttcgtgt cggtcgggtc ggccgcgacg cccccagcct
     1801 ccggtcctat cccgcgcacg ccggacggca agcctgactt ctcgggcgtg tacgcgtttg
     1861 gcggcggcgg ccggggccgc ggcgcgggcg ctcccgcgga cgggattgcg cgcaccccga
     1921 cgctgaagcc gggcgcggag caatatcgcg tcgcccgcgg cagtctcgac gtcggacgaa
     1981 cggccgactg catgccgctc agcccgccgg atgcgtttgg ggtgccgtac cagtttcaga
     2041 tcgtgcagaa caaggacctc ctcgtgatcc ttcacgagta cccaggcacg ttccgcctcg
     2101 ttccgctcga cggcgagccg caccaggtcg atcccgatcc gagctggctg ggcgattcgg
     2161 tggggcgctg ggagggcgac gtgctcgtcg tcgatacgat tggctacaac gacaagacgc
     2221 aggtgagcgg cttccggcac tcggaggcgc tccacaccgt cgaacggttc cgtcgcatcg
     2281 agggcgggtt cgagtacgac gtcactatcg aggatccgaa cgtcttcgtc gggccgtgga
     2341 ccgagacgag gacctaccgt ctcaacgtcc cgccgatgaa gaggatcatg gagttcgtct
     2401 gcgagaacaa ccgggactac cggccgctgt tcggcccgca gggcgcccag cccgctcagc
     2461 ccggccgctg atcgcgccgg gtcgcagcgc gtcagttgcc ggacggccgc gtcgcaggtg
     2521 tgtgtttcgg cagttgaccc gcaccgaacg acttgctgcc cgcgaccgag tcgcagtgct
     2581 tgcctggttc aggcttgctg ccctttggac acaggaacag tcccgactct cctctgtcgc
     2641 ctgccggctt cccatcccgc cgcggatgga gtcccatgct gaagacggtg ccttgaggga
     2701 acgcctctcg cgtgatgcca tccttgagcg cttgcgccgc gctctccatc tgaacgaccc
     2761 agacgaaggg ctcacccttc tgatcgcgca cgagagcgtc ccgtgcatcg ttcaagggaa
     2821 cgaaatggat ttcgaaatgg gccgcgttgg ggacaacgcg aacgatcact cccgtgaaga
     2881 cccgatagac ggcgccgtcg tacatcgcgt acgaatgatg agccgtggca cgcggcgtca
     2941 cgaacactgt caacacgagc gcgacgacta tcgaggccct atttgaagcc aaggtccagg
     3001 tccttcttcg ggcggtccat gttcttctcg aaatactctt cccagatctt tgcccatggc
     3061 cggtcgagca tgtccgggac tttgtactga atgacctgcc ccggcgcgac cggagtggcg
     3121 tagccgtcga tcgggtacag cgggcgggtg cattccgccc agccgagacg gtcggcggtg
     3181 ctccatcctt gctgatagtt cctgtgcgac aggatccgca ccggctgaac cagcgcgtcg
     3241 gcatcgtaaa tcaccgcttc ccagtcgatg ccgaggaatc gtccggcgga gtcgcgcacg
     3301 ggcgtcagga tctcgatcgt ctcgaggcga tcgctccact cccagctcga atgctgattc
     3361 cagccctgta cgttggcggt ccaggtgatg agcgcgtcgc catcccagaa tccgatcgtg
     3421 tctccgtacc actgcggaac gccctgcccc agagggaacg cccggtcgag gtgaacgacg
     3481 cgccacatgt tgccgctgcc gctgcccatg aagagcacga cctccggagt gaccacgatg
     3541 cggccgggct ttggaccggt ggcccactgg cgcatgaacc cttcgggcca gcagtacgac
     3601 gcggaccatt ggtgcgcggc gttcacgccc tcgtgataga gctgtcgcac catgcgctgt
     3661 tgatactccg gcgtcagcag cgacagcacg gtcggaacct gattggcgcg agagtagttc
     3721 cagacccgac gcccgttcgg cgcgtactcg ccgtacactc cgtcccacct gggcatcgtc
     3781 tcgtaggtat gtctcgtcgg cccgccccgt gatgtcgcgt cggcacgcag cgcctcgtag
     3841 tggtccttcg cggtcgtgaa acggtagggg ctgacgatgt tctcgcgagg ccagtccatc
     3901 gcgcagtctc cccagcgcgc ggagccgggc ggcttgctgc cgattcgcgg gtcgttcgtg
     3961 ctcgagccgg cgccgccgct ccgcatgtcg gcgacctgcc gtggggcgtt gcagcgccag
     4021 tagcgctggt ccatccagcg gtcgcgatcc ttgtagaagt ccttgctcgt gaagaggtct
     4081 gtagcgagcg gctgtacccc ttcgggcggc cgcccatccc actgcggtcc ctctccgtag
     4141 ctgcgcacct ccgggggtgg attcatggat ggaaacggcg cgccccgcag gcccgggacc
     4201 tcgggtggcc gcagttgagc gaacgccgtc ggcgttgcgc ccgcgatcac ggcgagcgag
     4261 aacaccgcaa cctcgaatgt cgaacgaact gcaggcatgg gctcctctct catcgcccct
     4321 ggacgggcgc gtcagagtgg cgaagtgcgg tcgcggacgt gataatagcg cgcctgggga
     4381 tctggagcgg gctcttccgc atttcaggag accgacgatg ctcacacgcc gctcggtggt
     4441 tgctctcgtc gtgctgtgtc ttgccgccct cgtccggccc gccctgcgtg aggtcgaacc
     4501 ccttgccctg agcggcgtcg aagcgacgct cctcgcggcc gtgccgtcgg cgaggccgga
     4561 ggacgtcggt ctgtccgccg agcgactgaa gcgcatcggc gagctgatgc agcggcacat
     4621 cgcggccaag acgtttcccg gcgccgtgac gctcgtcgcg cgcaacggcc gcattgccca
     4681 cttcgacgtg cacggcctga tggatctcga gacgcagaag ccgatgcaga aagacgcgat
     4741 cttccgcatc atgtcgatga cgaagccggt ggtcggcgtc gccatcctga tgctggtcga
     4801 ggaaggaaag gtcagactgg ctgaccctgc gtcgaagttc atcccggagc tgcagggcct
     4861 ttcggccgtg gttctcaaca ccgatggacg agtgtcgcct gcgccttcgg ggtcggtcgc
     4921 cgcgccgctg ccgggccgga cggttcccgc ggaccgggag gtcaccgttc gagacttgtt
     4981 gacgcacacg tcgggcctga tgagcggcgg cgcgagcgca gccagcacca acgacgtcat
     5041 gccgaaggcg ggcgagacag tcgctcaggt gatgccgcgc ctgaagaagg ttccgctcga
     5101 cttccagccc gggacgcgct gggcctacag tccgcagttc gggttcgacg tgctgcttcg
     5161 tgtcgtggag gtggcctccg ggatgccatt cgatcggttc gcgcgccagc gcatcttcga
     5221 gccgctcggc atgaaggaca cgttcttcta tccaacggac ggcaattccc ggatcgcgac
     5281 gctctacgaa caagccaacg gcgtcctgcg caaggcggcg aaccccgcat tcatgaacgg
     5341 cgcgtacgtg tcagggggcg gcgggctgtt cagcacagcg gaggactacc tgcagttcgc
     5401 gctgatgctc ctcaatcggg gtgagctcga tggcaagcgc ctcctgagcg tcaggacggt
     5461 ggacctgatg gccacgtcgt tcgcaccgga cacgtttccc ggccggctgg cgggagaggg
     5521 attcgggttg agcgtgcgcg tcgtcgacaa tcccgcggcg cgcaccacgt atctgtcgaa
     5581 ggggagcttc gggtggagcg gcgccttcaa cacgcatttc ttcatcgacc cggccgagcg
     5641 catcgtgggc ttgttcatga cgcagatcgt ggggctctcg acgcgcggcg acgtccggga
     5701 cgatttcgag acggccgtca tgcagtcgat cgtcgggagc ggcgctccct ctggctcgaa
     5761 ctgaggacgg cgtctccttt cgatcaggaa ggcccgctca tgtggaggga tctgaaatca
     5821 gtcctgcgga cgctgattcg gcagccgctc gtcgccacgg tcgtcgttgg aacagtggcg
     5881 ctcggaattg gcggcagcgt cgccgtgttc agcgtggtca acgccgtgct gatccgcgag
     5941 cttccatacg cggacgcggg acggttgtac gtcatgcgtg cggttgcgcc ggacgggcga
     6001 ctgggcaaca tcacccacag ggagttcaca ccgatctatc agaacgacca gcacgccatc
     6061 gtcgagaagg cggccatcgt ctggagccag gattcgcaga tcgtgggcgc cgacaagaga
     6121 ccccatctga ccgtgcgata tggagtgacc gaccggttct tcgacgtgtt tggcacccgg
     6181 atggccatcg gctccggttt tcggccggga gacctgcccg ggcgcatcgt gatctcctat
     6241 ccgatctggc gcgacgcgtt tggctccgat ccgaacatca tcggcaagcg cgtcgtggcg
     6301 gaaggcatga ccctggaagt cgtcggcgtg acgccggccg atttcgattt tccggaagat
     6361 ccggggttct ggtacctcat gcggctcggg agcggattcg acaaagtccg agggtatcgg
     6421 ggattcatgc ggctccggga gggcagcacc ccggagcaga tcgacgcgga gatgaagcgc
     6481 gtggccacga cgctcggcca ggacccggtg acgaaacagg tgccgatcct cgtcgcgcag
     6541 ccgctgctcg agcatgtcgt cggcaacgac ctgaagtcca ccgtgatgat cgtcttcgga
     6601 gcgaccggca tcctgctcct cattgcctgc atcaacgtca cgaacctgct gttgtcgcgg
     6661 gcgacggtcc ggtcccggga agttgcgctg cgtgaagcgc tgggcgccgg ccggtggcgg
     6721 atcctccgcc cgttcctgat cgaaagcctc gtcctcacga cggtcggcgg ggcgctcgga
     6781 ctggccgcgg ccgctgcggg cattagagtg ttcctcgcag ttgcgccgac cgggctgccg
     6841 cggctcgaca cggtgccgat cgacgggacc gtgctgatct tcgcagcagg cgcgacgctg
     6901 ctgaccggtg tcctcgttgg cctggcgccc gccgttcggc tcgctcgaaa ccagcttcgc
     6961 agtctcatca acgagggcgg ccgcggcgct tcgggagggc catcacagaa tcgggtgttc
     7021 agcgtgctcg tcgtgaccga gaccgcgctc gccgtcgtcc tcgtggttgg agccggactt
     7081 ctcgttcgga gctactacaa cctgacgacg accgatcctg gtttcaatcc ggatcgggta
     7141 ctgaccgtat cgatgaacgt cccgggacgc atgacggcgt cggtcgtcca gaatgcccag
     7201 ggcaagcccg aattccgcgc ctcgtacgct ccgatggcga gcttcttccg tgagctcgaa
     7261 cagcgcctgg gcggaatcac cggtgtgtcg ggtgtcgcga cgacaaccct ccttccgctc
     7321 gatcccgact ctgccggcgt ctcgatcgac ttcacccttc ctgagcgcac cggcgccaca
     7381 cccgacgccg ccgcctggac gagcccccta cactccgtga gtccagagtt cttcgacaca
     7441 atgggcatga gactgctcac tggccggaac atcacgaggt cggatcaaca aggctcgccg
     7501 ggtgccgccg tcgtcaacga gacgttcgtg cgccgtttcc tctccggcgg cagcccgctc
     7561 ggcgaacgca tccggtggcg cctgaatcgc tacgtcccga cagacacagg attccagttc
     7621 ggacacctga ccgtggaaga agtcgaggtg gtcggcgtcg tgaccgacgc caagtacgcg
     7681 tcgctcgcca aaccagccga gccggccatc tacgtgtcga gcgagcaatt catcttccgc
     7741 cggcggacag ttgccgtgcg cgcggccggc gacaacccgg agagcctcgt gtcgcccgtt
     7801 cggcgagagc ttgccgccat ggatccgctg atcggcgccc ggtttgccgc gtacgactcg
     7861 atcgtgcacg actcgatcgc ccgccagcgg ctgggcatgt cgctgctcgt cgccttcggg
     7921 ctcgcggcgc tcctgctggc ggcggtcggc gtctacggtc tgctgtcgta ttcggtcgcg
     7981 cagcgaacgg gcgagatcgc ggtgcgttcg gcgatgggat cctcggcgac tcaggtgatg
     8041 agactcgtga tggggaaggg catgcgcctg gcggtggcag gcattgtcat cggggctctg
     8101 ggcgcggtgg cgcttcgtca ggtcgtggcg ggacagttgt acggcgtgag cccgctggat
     8161 ctctccgtgt tcctcctcgt gatcatcgtg ctcctcggcg tctcggtcct cgcgtgcgtc
     8221 gtccctgcgc ggcgagccac gagaatcgac ccggtcacgt tgttgaaggc ggagtgaccg
     8281 aggcccgaag ccgtacgccg atcacgccag accctgcatc cgttgacccc ttcggcggcg
     8341 actaaggagc agggagatca gagatgagaa atctcgtact actcgctttc ctgctcggcg
     8401 tgccgtccgt ggcgttcggg cagaccgcgg atgagatcgt cgccgccaac atcacggcgt
     8461 ccggtggcga ggccgccatt gccagaatcc agaacatcac caccaagggc cgagtcacga
     8521 tcgagagcaa gttcttcgga aaactcgaag gcacgctgga gacagtccgc gtgccggggc
     8581 gcggctacta tgaacgcgcc gcgctgggtc cgatcgagca gaccaagggc tgggatggca
     8641 ctcgtggctg ggaacaggga ccgaacgggt cgaggacgct cgatggcttc gagctcgacg
     8701 tgatgcgggt gcagtcgttc gtgaacccgt ttgttgcgca gcgcacgctg gcgccgccgg
     8761 gcctgcgcat tgaacggctc gacgacgcgc cggtcgacgg caggccgcac tacgtgctga
     8821 tcgtcaagac gtccgagggg ccgccggcga cgatgtacat cgatcgcgag acgaaactgc
     8881 tcacgcgcag catcatgacc gtctcgatcc cgaacgttgg cgaggcgacg atcgtcactg
     8941 acgtcggcag ctacaagccg gttgccggcg tgatgatgtc gaccacgttg acgcaggtgg
     9001 ctgagggact cgcgacgacg accgtgatac tcgacgacac ggccgtcaac accgccatgg
     9061 acgcgaagat cttcgcgtcg cgctgaagag gcttcgacat ggactttgaa ggtgccttcc
     9121 tcgcctggac tgcgctggcg atcttcattc cgctggctct cagcgtggcc gttccggggg
     9181 tctatcgtac gtgggtcgag taccgctcgc gcagccaggc gctcgacgtg ctgcgcgtct
     9241 acgccgagaa gggccaggag ccgcccgcct cggtcatggg ggccctcgcg gttgcggcgg
     9301 ctccgacggc gcctgctgcc cacgcctctt taccccccag cgtgtttccc ggaccaacgc
     9361 gtgagaagca cctggcgcat ctggcgggaa gtctcgtgtg cggactggga gcgaccggag
     9421 tcgcctggtg gcggtggcct gcagatggca acccggggcc gctcgtcatc tgggccgtcg
     9481 tcatcgcgat cttcttcgcc ggcggtgttg ccgcgcggct cgtcggcgtg ttcaccacgc
     9541 cgagcggccg gctgcgcgat ggccgatgac gacgtcgcgc tcatcgccga cattcggaac
     9601 ggatcggagc gcgccttcaa cgtcctgatc gaccgccatc agcaggcggt acgggatttt
     9661 ctgcgccgcc tgctggccga cgcgtcggag gctgacgatc tggcacagga gacgttcatg
     9721 gtggcatgga cacgtccggg gacctaccgc ggcgacgcca gcgttcgatc ctggctctgc
     9781 gcgattgcgt ggcgcaaagc ca
//
LOCUS       DBNGSH010000624         9308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1421020, whole genome shotgun sequence.
ACCESSION   DBNGSH010000624 DBNGSH010000000
VERSION     DBNGSH010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9308
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1421020"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="AC16CY_14435"
     CDS             <1..1184
                     /locus_tag="AC16CY_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3355044.1"
                     /translation="ALQATVARPLSGRPNHFVAGVSFDADGGGTEYEADTEIARLTED
                     RGTEGIGQFDSDAAVRLRTTVRHAGVYAADFFTVAPSLTVMGAARFNYSDIALRDQAG
                     TALDGDHEFARLNPAAGITWDLPRGATAYGSFSVSSRVPAPSELSCADAADPCRLPNA
                     FVADPPLEQVVARTWEAGIRGRRKAVSWNASFFRTATIDDILFISSGPLTNTGHFQNV
                     GDTSRQGLELGASGVLRAVRWDVAYSLIAARFDTPLTLSSPNHPDAEDGEIRVEAGSR
                     IPGVPRHHFKLNLSRTIGRFTIGGNLLAESSQYFRGDEANLLEPLDGFAVVNLTAGYA
                     LARRVAVTARVTNLFGAGYSTFGLLGDADDVLGAEYADPRFVSPGAPRAAWVGLQFSF
                     R"
     gene            1742..2146
                     /locus_tag="AC16CY_14440"
     CDS             1742..2146
                     /locus_tag="AC16CY_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3355045.1"
                     /translation="MPANVVIDTSAILAVLLDEPEKQAVVEATMGSVVCAPPSLRWEV
                     GNAATAGVKRRRLTSERARQLVTDFEQVTIRELAIDILRAVNLGLELGIYAYDAYILE
                     GARSSGFPLLALDGPIRRNAKQLGLTLIEVDL"
     gene            2143..2436
                     /locus_tag="AC16CY_14445"
     CDS             2143..2436
                     /locus_tag="AC16CY_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3355046.1"
                     /translation="MKLYTYSTARQRLAEVLEEASRDGEVQIRRQDGRVYAVTPVAKR
                     GRSPFANVTGRAVRGVTSKDLIRLAREEGWARGARVVRSARASRAARGKSRAG"
     gene            2640..3272
                     /locus_tag="AC16CY_14450"
     CDS             2640..3272
                     /locus_tag="AC16CY_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3355047.1"
                     /translation="MWQLAPRINLGRALQLKTQQLMRPQTRTSVTQLLEAWRGGDADA
                     ADQLFALVYDDLRALARRQLARLRPGETLAPTVLVHEAYLKFAERSAPAVVDRPHFLA
                     VAARAMRLIVIDHIRRRRARKRDAGGGGPIALSSSVAAPTEMALTDLIAMNDALDQLA
                     ALDPRQVQVVELRFFAGLELKEIAAMLETSERTVKRDWQRARAFLYASLR"
     gene            3269..6196
                     /locus_tag="AC16CY_14455"
     CDS             3269..6196
                     /locus_tag="AC16CY_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355048.1"
                     /translation="MTPTPDSWRRICAVLDRVSELDPRLRVERMAEACAAEGISVDDV
                     TPYLAAEYEARLPEQIDPDLRDEALRGLALGHDASPAAPGEHLGPYEVIAQLGAGGMG
                     EVYKARDTRLDRMVALKWLHGDLAARPEARLRFQREARAISALNHPHICTLFDIGEHD
                     GVDFLVMELLEGETLAARLRRGALPMADAVEFAAQIADAVGAAHRRGFVHRDLKPANI
                     MITPAGVKLLDFGLAALRHDREWLEGGTTEALTAEGTLVGTVPYMAPEQLQGQTVDGR
                     TDIFALGAVLYEMLTGRRPFERDGTAQSLAAVLESEPVAVDEVRPEVPQPLAWAIGRC
                     LIKDRERRWESAGDLSRFLRDLSIARRTVQVRASKRRRYLVAVLLAATLVLAAAWASL
                     LLRRGAVATAPPALTRFEIFPPNGYSFDRLPALSPDGSRLAFTASRGGERTLWLRAID
                     ALTPQRLAGTDGATYPFWSPDGRFIGFFAGNALKRIELATGRVETICNCDTGTGGGGT
                     WNRDGVILFSKGLVVSPLWQVPATGGLAVALPPTAPGGGSDIERIGANAWPQFLPDGR
                     HFLFFTGGQSTAGVYVGVLGAKDYKPVLRFGRGLLGDAEPGAGATANQTFRTRGWYSS
                     GYLFFLQQRSVVAQRFDLDRLEVAGSPIRLVEEVEQTAPGRAFFSVAGNVLAYLPRVE
                     DRTLLRLTWIDRAGNDLAALDDGPYNGIALSDDGRLVLAATAGSGVLRVDTASGIPTP
                     TGMQGQSPVWAPGGSRFIAAGGPAGRPFPVIVDLATGDRKVLDVSLGGQAWATDWSHD
                     GRYVVGHMLNSETLLDIWAADVVATPPKLWYLARAAGNQHDQRISPDGRWVAYASNEG
                     SNTFEVYVRRFPEGPGSWRVSMAGGRLPTWTSDGRALLYVAPDGTVMETKLTSGAEFR
                     STAPQPLFRHAALARAYDRDAQFGRAYDTRDGRRFLVAVPVSEPPPAPLVVVTNWERM
                     LPP"
     gene            6308..7840
                     /locus_tag="AC16CY_14460"
     CDS             6308..7840
                     /locus_tag="AC16CY_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355049.1"
                     /translation="MPRMPTYALVLVVGLGAVAFAQRPGVPRVWDDAALEDWATPIAG
                     LNTRPAHYRAAEYYAAPAVNLRTYPLYHPDSEPAGYWEWLQQQKPEPLVDATRMRTNL
                     DWVAAGERAFVEMDAVLMRIDDPAVIARARDPRAFDGMFKSSDGAAFGIRWVVTESGL
                     MLTAQACGACHAAVDPDNRVTVPGPLGTRLTERGQRGVRGLPLAGAQGTARRLQQFYT
                     GDSPGVALWREFTVPWAPDERVERFAMMSAERLQQRNVDERLGQSFGNGVFARTNGSP
                     YAATKIPDLMNLRYYRYLDATGSHRLRGPEDIARYAALVTGADRMDFGPHRILTDAQR
                     RVRFRHADEVLYAIGVYLMSLEPPKNPEVASPDVVARGETIFRREGCVGCHAPPNYTN
                     GKLTLAEGYTPPADHPNREDIMPRTVGTDPDAAMKTRKGTGFYKIPSLRGVWFRRSLL
                     HDGSIATLEELFDPARLQPDYQSKGWNPPGADRRAIKGHTFGVSLPPADRVALLAFLR
                     SL"
     gene            7980..8663
                     /locus_tag="AC16CY_14465"
     CDS             7980..8663
                     /locus_tag="AC16CY_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355050.1"
                     /translation="MRVTTRFAVVVMLMAIVGFTLSIVVPSPARGAGSAPVTVVNTPA
                     EPVPVALQGTGSVAGTVNALQSGPWTVGVSSLPAVQLAPGTTFNLNSSPTAPIYTADV
                     SARQAFAVSVCDGSGNSTCPSGEVAFVPVGKRFVIEQVSGRCWSNGDVPATFYIYASL
                     NGQAYPYYTSGVQGLVGVGQTNYIIVGALTRIYAETSVGIARFDSSTEECRATLSGHL
                     QDNSSPFPG"
     gene            8922..9016
                     /locus_tag="AC16CY_14470"
     tRNA            8922..9016
                     /locus_tag="AC16CY_14470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8956..8958,aa:Ser,seq:gct)
     gene            9098..>9308
                     /locus_tag="AC16CY_14475"
     CDS             9098..>9308
                     /locus_tag="AC16CY_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR3355051.1"
                     /translation="MTKTIVVACSVLALSFPLLASAQVTQVDVTGGRVAGTPANGIVS
                     FKGIPFAAPPVGALRWKAPQPVASWT"
BASE COUNT         1546 a         2941 c         3168 g         1653 t
ORIGIN      
        1 gcgctctgca ggcgactgtg gccaggccgt tgtctgggcg cccgaaccac ttcgtcgccg
       61 gcgtcagttt cgacgccgac ggcggaggca ccgagtacga ggccgacacc gagatcgcgc
      121 ggctcacgga ggaccgggga accgaaggca tcgggcagtt cgacagcgac gccgcggtcc
      181 gcctcaggac gacggtccgg cacgcgggtg tgtacgcggc ggacttcttc accgttgcgc
      241 cgagtctcac ggtgatgggg gcggcgcgtt tcaactacag cgacatcgcg ttgagagatc
      301 aggcgggaac cgcgctcgac ggcgaccacg agttcgcgag actcaacccc gccgcgggga
      361 tcacgtggga tctgccgcgc ggcgccacgg cctacgggag cttcagcgtg tcgtcgcgcg
      421 tgccggcgcc gtcggagttg agctgtgccg atgcggcaga tccctgccgc ctgccgaatg
      481 cgtttgtcgc ggatccgcct ctcgagcagg tcgtggcccg cacctgggag gccgggatcc
      541 gcgggcgccg caaggcggtc tcgtggaacg cgtcgttctt tcgcaccgcc acgatcgacg
      601 acatcctgtt catcagcagc ggcccgctca ccaacaccgg gcacttccag aacgtcggcg
      661 acacgagccg gcagggcctg gagcttggcg cgtcaggtgt gctccgcgcc gttcgctggg
      721 acgtcgccta ctcgttgatc gccgctcgct tcgacacgcc gctcaccctc agcagcccga
      781 atcatcctga cgcggaggac ggggagatcc gggtggaggc cggcagccgg attccggggg
      841 tgccgcggca ccacttcaag ctcaatctct cgcgcacgat cggccggttc acgatcggcg
      901 gcaacctgct tgcggagtcc agccagtact tccggggcga tgaagcgaac ctgctcgaac
      961 cgctcgacgg cttcgccgtc gtcaacctga ccgcgggcta cgcgctcgcg cgccgcgttg
     1021 ccgtgaccgc gcgcgtcacg aacctgttcg gtgccgggta ttcgaccttc gggctgctgg
     1081 gcgacgccga tgacgtgctc ggcgccgagt acgccgaccc gcgattcgtc agccccggcg
     1141 cgccgcgcgc ggcgtgggtg ggccttcagt tctcgttccg ctagaggtcg atgggcggtg
     1201 gacgcgtggt gccaacacct tcctggcacg gtgccgagca accaatcggt ctgaagcgcc
     1261 ccgccattgt ggccgcgacc gggatggtcg gcgggtatgc gttgcgttac gcggttgatc
     1321 acacggctgt cggacgcgtc acagcgatcg ggcgcagggc gctcggcatc tcgcatccca
     1381 aactgaaaga ggttctgcac cgcgacttcg cggactgttc ggcgctcggg gaaacgctct
     1441 cgagtcagga tgcagccatc ttttgtcttg gagcgtacac gggcgccgtg ccggatgcag
     1501 agctccacag gatcaccgtg gacgacacgg tcgagttcgc ccgagtgttc cgcgcgagca
     1561 actcggacgc tgcgttctca ttcttgagcg ggagcggtgc ggacccgacg gggcgcagcc
     1621 ggctggttca tttcgaacgg ccggccacca gcggtggaga ggtctggtct cctcagagat
     1681 cgggaacggg catacagact gggcagcaga atcattgtac aattgtacga actgtggcgc
     1741 gatgccagcg aacgtcgtga tcgacacgtc agccattctg gccgtgctcc tcgatgagcc
     1801 tgagaaacag gcggtcgtcg aggcgacgat ggggagcgtc gtgtgcgcgc cgccatccct
     1861 gcgctgggag gtcgggaacg ccgcaaccgc cggcgttaag aggcgccggc tcacgtcgga
     1921 gcgtgcgcgg caactggtca ccgacttcga gcaggtgacc attcgagagc ttgcgatcga
     1981 catcctgcgt gccgtgaatc tgggtctgga gttgggcatc tacgcgtacg acgcgtacat
     2041 tctggaagga gcccggtcat ctggattccc gctgctcgca ctcgacggtc cgatcaggcg
     2101 gaatgccaag cagctcggct tgacattgat cgaggtggat ctatgaagct ctatacatac
     2161 tcgacggcac ggcagcggct cgccgaggtg ctcgaggagg cgtcacgcga cggagaagtc
     2221 cagatccggc gtcaggacgg tcgcgtgtac gccgtgacgc ccgtcgcgaa gcgaggtcgg
     2281 tcgccgttcg cgaatgtcac cgggcgggcg gtcaggggtg tgacgtcgaa ggatctgatc
     2341 cggctggcac gcgaggaagg atgggcacgt ggcgcacgtg tggtgcgcag tgcccgcgcg
     2401 agtcgcgcgg cgcgtggcaa gagccgtgcg ggttgagcgt gagtcagtgg gccgaccgga
     2461 tcggcaatcg accggatcgc gcaatcgagc gtgcaggtcg gcatctccag gccgaataca
     2521 actaagtaat gaaccaaacc ggagaaatag agagcccgca tcggcgcgcg taactggcca
     2581 cgccgtcgcc gaattccagc ccacgtaata ctgccttaga cgagtcacgt ggcacttctt
     2641 tgtggcagtt ggcgccgcgc ataaacttgg gccgtgctct ccaactcaag actcagcagt
     2701 tgatgcggcc ccagacgcgt acgagcgtta cacagttact ggaggcgtgg cgtggagggg
     2761 atgccgacgc tgcggaccag ctattcgcgc tggtttatga tgacctccgc gcgttggcca
     2821 ggcgacagct cgcacgactg aggcctggag agacgctggc gccgaccgtg ctggtccatg
     2881 aggcgtatct caagttcgca gaacgatcgg cgccggccgt cgtcgaccga ccgcatttcc
     2941 tcgccgttgc agcacgtgcc atgcgactca tcgtgatcga tcacatccgc cgccgtcggg
     3001 cgcgaaagcg tgacgctggc ggcggcgggc cgatcgcgct gtcgagctcc gttgcagcgc
     3061 cgactgagat ggcactgacg gatctgattg cgatgaacga cgcgctcgac cagctcgcgg
     3121 ccctggaccc gcggcaggtg caggtcgtcg agctacgatt cttcgccggc ctcgagttga
     3181 aggagattgc cgcgatgctc gaaacttccg agcgcacggt caagcgtgac tggcaaaggg
     3241 ctcgcgcgtt cctgtacgcc tcgttgcgat gacgccaacg ccagacagct ggcggcgtat
     3301 ctgcgcggtc cttgatcgcg tcagcgagct cgacccgcgt ctccgcgtgg agaggatggc
     3361 ggaggcgtgt gccgcggaag gcatctcggt cgacgacgtt acgccctacc tcgcagccga
     3421 atacgaggcg cgattgcccg aacagatcga tcccgatctt cgggacgaag cacttcgagg
     3481 gttggcgttg ggacacgatg cgtcgcccgc tgcgccaggc gagcacttgg gaccctatga
     3541 ggtgatcgcc cagctcgggg ccggcggcat gggcgaggtg tacaaggccc gcgacacgcg
     3601 actcgatcgc atggtcgcct tgaaatggct gcacggtgac ctggccgcgc gtcccgaggc
     3661 gcggctgcgt tttcaacgcg aggcgcgcgc gatttcggcg ctcaaccatc cgcacatctg
     3721 cacgctgttc gatatcggcg agcatgacgg cgtcgacttt ctcgtgatgg agcttctgga
     3781 gggtgaaacg ctcgcggcca ggcttcgccg cggagccctc ccgatggccg acgcggtcga
     3841 gttcgcggcg cagattgccg acgcggtcgg ggccgcgcat cggcggggat tcgtccatcg
     3901 cgatttgaaa ccggccaaca tcatgatcac tccggctgga gtgaagctcc tcgattttgg
     3961 tctggcagcg ttacggcacg atcgcgagtg gttggagggc ggcacaacgg aggcgctgac
     4021 cgctgaagga accctggtgg gaacggtccc gtacatggcc ccggaacagc tccagggcca
     4081 gaccgtcgac gggcgcaccg atatcttcgc gctgggcgca gtgctatacg agatgctgac
     4141 cggacgacgc ccattcgaga gggacggaac ggcgcagtcg ttggctgctg tgctcgagag
     4201 cgagccggtg gcggtggacg aggtgaggcc ggaggtgccg cagcctctcg catgggcgat
     4261 cggccgatgc ctgataaagg accgtgagcg gcgctgggaa agcgcgggtg atttgtcgcg
     4321 tttcctgcgc gatctcagca tcgcacggcg gaccgttcaa gtgcgtgcgt caaagcggcg
     4381 acggtacctc gtcgctgtgc tgctcgcggc aactctcgtc ctcgcggccg catgggcctc
     4441 gctcctgctg cggcgcggcg ctgtcgcgac agcgccgcct gcgctgacgc gattcgagat
     4501 cttcccgcca aacggctaca gcttcgatcg tctgcctgcg ttgtcgccgg acggcagtcg
     4561 gctcgcattc actgcgtcgc gtggtgggga acgcacgctg tggctgcgag cgatcgacgc
     4621 gctgaccccg caacgattgg ccggtactga tggcgcgacg tacccattct ggtcaccgga
     4681 tggacggttc ataggcttct tcgcgggcaa cgcgctgaag aggatcgagc tggcgacggg
     4741 acgcgtggaa acgatctgca actgcgacac cggcaccggt ggcggcggca catggaaccg
     4801 tgacggtgtc atcctctttt cgaaagggct ggtggtctcg ccgctctggc aggtaccggc
     4861 gactggtggc cttgctgtag cgctgccacc cacggctccc ggcggcggca gcgacatcga
     4921 acgcatcggg gcgaacgcgt ggccgcaatt cctgcccgat ggccggcatt ttctgttttt
     4981 caccggtggg cagagcacgg ccggcgtcta tgtcggagtc ctcggcgcga aggactacaa
     5041 accggtgctg cggttcggac gcgggcttct cggcgatgcc gagcccggtg ccggcgcgac
     5101 tgccaatcag acgtttcgca cgagaggctg gtattccagc ggttatctct tttttttgca
     5161 gcagcgttcg gtcgtcgcgc agcgattcga tctcgatcgc ctggaggtgg caggcagtcc
     5221 gatccgtctc gtcgaagagg tcgagcaaac ggcgcccggc cgggccttct tctcggtggc
     5281 tggcaacgtg ctcgcctatc tgcctcgagt cgaggacagg acgcttctac ggctcacgtg
     5341 gatcgatcgg gctggtaacg atctcgccgc actcgacgac gggccgtaca acgggatcgc
     5401 gctgtccgat gatggccgtc tggtgctggc ggccaccgca ggcagcggcg tgctccgggt
     5461 cgacacggcg agtggcatcc cgaccccgac cggtatgcag ggacaaagcc ctgtctgggc
     5521 tcccggcggc tcgcgcttca tagcggccgg tggtccagcc ggcagaccgt ttccagtcat
     5581 cgtggatctt gcgacgggcg accgcaaagt gctcgatgtg tcgctcggcg gtcaagcctg
     5641 ggcaaccgac tggtctcacg acggtcgcta cgttgtcggg cacatgctga attcggagac
     5701 gctgctcgac atctgggcgg cagatgtcgt ggccacgccg ccgaaactgt ggtacctggc
     5761 acgggcggct ggcaaccagc acgatcaacg catctcgccc gacggacggt gggtggcgta
     5821 cgcctcgaac gagggctcga atacgttcga ggtgtacgtg cggcggttcc ctgagggacc
     5881 cggaagctgg cgggtgtcga tggccggagg gcgactaccc acgtggacgt ccgatggtcg
     5941 tgccttgctg tacgtcgcac cggacggcac cgtgatggaa acgaaactga catccggcgc
     6001 cgagttccgc tctaccgcgc cacagccact cttccgtcac gccgcgctcg cacgtgccta
     6061 cgaccgcgac gcacagtttg gccgcgctta cgatacgcgc gacggccggc gcttcctggt
     6121 ggccgttccc gtcagcgagc caccgcccgc accgctggtc gtggtcacga actgggagcg
     6181 gatgctgccc ccctgatttc ctgtctttcc tagcaaatag ctgagctgtc ccttccggcg
     6241 ccctttctgc ggatcaccag gtagcgcgcg cttcgtgcgg cgccgctacg gtgaccggga
     6301 ggaactcatg ccgcgtatgc caacgtatgc gctggtgctc gtcgtcggcc ttggggccgt
     6361 cgccttcgcc cagaggccgg gcgttcctcg ggtctgggac gatgccgcgc tggaggactg
     6421 ggcaacgcca atcgcgggct tgaacacgcg ccccgcgcac taccgggccg ccgaatacta
     6481 cgcggcaccc gccgtgaacc tgcggaccta tcccctgtat cacccggaca gcgaaccggc
     6541 cggctattgg gaatggctgc aacagcagaa gcccgagccc ctggtcgacg cgacgcgcat
     6601 gcggaccaac ctggattggg tcgccgctgg cgagcgcgcg ttcgtcgaga tggacgctgt
     6661 gctgatgcgc atcgacgacc ccgccgtgat cgccagggct cgagacccac gcgcgttcga
     6721 cggcatgttc aagtcgtccg acggggccgc gttcggcatc cggtgggtgg ttaccgaaag
     6781 cggcctcatg ctgacggcgc aggcgtgcgg agcgtgtcac gcggccgtcg atccagacaa
     6841 tcgcgtgacg gttcccggtc cgctgggcac gaggctgacc gaacggggcc aacgaggtgt
     6901 gcgggggctg ccgttggccg gcgcgcaagg cacggcgagg cgcctacagc agttctacac
     6961 cggcgattca ccaggcgtgg cattgtggcg cgagttcacg gtgccttggg cgcccgacga
     7021 gcgcgtcgag cgcttcgcca tgatgtcggc ggaacgactt caacagcgca acgtcgatga
     7081 gcggctgggg cagtcgttcg gcaacggcgt cttcgcgcgg accaatggca gtccctacgc
     7141 ggcgacgaag attcccgacc tgatgaacct ccgatactac cgctatctcg atgccacggg
     7201 ttcccaccgg ctgcgcggac ccgaagacat cgcgcgctat gccgcgctcg ttacgggcgc
     7261 cgaccggatg gatttcggac cgcaccggat tctgactgac gcgcagcgac gcgtgcggtt
     7321 ccgccacgcc gacgaagtgc tgtacgcgat cggcgtgtat ctgatgtcgc tggagccgcc
     7381 aaagaatccc gaggttgcgt cacccgacgt ggtggcgcgc ggcgaaacga tcttccgccg
     7441 tgaaggttgc gtggggtgcc acgcgccacc gaactacacg aatggaaagc tcacgctcgc
     7501 agagggttac acgccgccag cggaccatcc caatcgcgag gacatcatgc cgcgcacggt
     7561 cggcaccgat ccggatgcgg cgatgaagac acgaaaggga acgggcttct acaagatccc
     7621 gtcgttgcgt ggcgtatggt tccggcggtc gctgcttcac gacgggtcga tcgcaacgct
     7681 cgaggagctg ttcgaccctg ccaggctcca acccgactac cagtcgaaag gatggaaccc
     7741 tcccggagcc gacaggcgcg cgatcaaggg ccataccttc ggcgtctcgc tgccgccagc
     7801 ggacagggtc gccctgctgg cattcctgcg cagcctttga ggtcgcgtgg caactcgttg
     7861 ctcgtttggt cggatctctc atcgacgtga agtggtggcg ggagccacct gcggcctcaa
     7921 tcttaatcag gcaaagtcgg gtgacggata gttccagcgc gtaacccggg aggagcccca
     7981 tgcgagtcac aacacggttt gcagtcgtcg tcatgttgat ggccatcgtc ggattcaccc
     8041 tctccatcgt tgtgccatcg ccggcacgcg gagccgggtc ggctccggta actgtcgtga
     8101 acactccggc agagcccgtt ccagtcgcgc tgcaagggac gggcagcgtg gccggcacgg
     8161 tgaacgcgtt acagagcggt ccgtggaccg tgggcgtctc aagtcttcct gcggtgcagt
     8221 tggctcctgg gacaaccttc aatctgaaca gctcgccgac ggcgccgatc tacaccgcgg
     8281 acgtttccgc cagacaggcc ttcgcggtga gcgtttgtga cggaagtggt aatagtacat
     8341 gcccctccgg cgaagtcgct ttcgtgccgg tggggaaacg attcgtgatc gaacaggtct
     8401 caggtagatg ctggagcaac ggtgacgtcc ccgcgacctt ctatatctat gcgtcgttga
     8461 atgggcaggc ctacccttac tacaccagcg gcgtgcaggg acttgttggc gtgggccaga
     8521 ccaattacat catcgtcggc gcactcacgc gcatatatgc cgagacgtca gtgggaatcg
     8581 ctcgatttga ttcttccacg gaggagtgcc gggcgacgtt atcgggtcat ctccaggaca
     8641 actcgtcgcc atttcctggc tgaggggcac agccttggtg accgtttgcg agtggcgccc
     8701 ggaagagccc ggcgaactac tgcacaactt gccgatccca gacgctggcg gctgactcgc
     8761 ggaatcattg gcgatttctt cgatgcagtg tggtgaggtg aacaccgagc cgcgaacgga
     8821 gatgtcgaga tttctggccg cgctggtcgc ggaccgaaaa accgtcgccc tcgaccgggg
     8881 cgtttgcgca agtgccgatc ttccgtatac ttaccgtctg cggtgaggtg gccgagaggc
     8941 tgaaggcggc ggtttgctaa accgttgtag ggcctttaaa gtcctaccca gggttcgaat
     9001 ccctgcctca ccgccagcct tcgctcgccg tcgctcgagc gagcttcggc tggcaagcca
     9061 tccgacgtcg ggactcttca caatgacggg tgatgtcgtg acaaagacga ttgttgtcgc
     9121 ctgctcggtg ctggcgctgt cgttcccatt gctggcgagc gctcaagtca cgcaagtcga
     9181 cgtgaccggc ggccgcgttg ccggcacgcc ggccaacggc atcgtctcgt tcaagggcat
     9241 tccgttcgcc gcgcccccgg tcggcgcgct gcgatggaag gcgcctcagc ctgtcgcgtc
     9301 gtggacgg
//
LOCUS       DBNGSH010000625         6368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2610159, whole genome shotgun sequence.
ACCESSION   DBNGSH010000625 DBNGSH010000000
VERSION     DBNGSH010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2610159"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..701
                     /locus_tag="AC16CY_14480"
     CDS             309..701
                     /locus_tag="AC16CY_14480"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355052.1"
                     /translation="MRYRLRSLFVAVVIAVTAAAMQAHHSVPGQFDMSKPMTLKGVIT
                     KVDWINPHIYVHLNVKEAAGTTTTWALATLPTAMMRRAGLTRESLQGKPGEEVTIDAL
                     PARDGTKHLAWINKITYADGHSFAPTGQ"
     gene            736..1593
                     /locus_tag="AC16CY_14485"
     CDS             736..1593
                     /locus_tag="AC16CY_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355053.1"
                     /translation="MTRILVSSIVSLSVLAAAGTSFAQVAARAPDRGTVKQLQTLSAK
                     PTPRLADGRTDFNGTWDHLGGIEFVRPVKKADGSICLIGCGPAPAGAPPAPFPPVQTD
                     FPKYKPEFQAKVADLSKRQVQVDTNLKCQAPGVPRIGPPAKIVQSAREVVFLYDDVNG
                     AFFRIVPIDGRPHRKDVPPSYLGDAVGRIEGDTLIVETVGMNDETWLTDNGAFHTKNL
                     RVTERFRRIGDTIEWRATVHDPEVLVEPWSLKPQVMWITDIEIEEPARCEDRDVPHIV
                     DGSHHDNPR"
     gene            1593..2537
                     /locus_tag="AC16CY_14490"
     CDS             1593..2537
                     /locus_tag="AC16CY_14490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3355054.1"
                     /translation="MKTSCARAASYQEFAALLASTETTADLFDLFLGHLDLIEQADPH
                     LVIGGQTSRSWRQFALGSIARLARLMVARRQPVDAKANYERRWSHHGAKSHRKRLLKP
                     RDYLIGDRIHTISEGTFSVLLTQTLITLFKQFIRQDDGVVVELGCGNGRNLFVIRSLF
                     PSVECIGVDISNAGPEFASAMGQQLDADGMTFLGDIDITDGALMSRVGAMLRGRSVVV
                     ITIGCLEVVPETARAIEQILALKPRCVIHFEPVLELHTGVFPSRDYFARRHLIAGGYM
                     TSLLTILERHDRIEIVTRRRAGLGKALLEYSVLVWKPK"
     gene            2574..2849
                     /locus_tag="AC16CY_14495"
     CDS             2574..2849
                     /locus_tag="AC16CY_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR3355055.1"
                     /translation="MVKVTFTLDDETVSTLRKTAERLARPQSAVVREAIHEYGARAGR
                     LSEDERRRMLRSIDAVLAQPPTRTIAAVERELRELRAARRGGGRRSA"
     gene            2846..3226
                     /locus_tag="AC16CY_14500"
     CDS             2846..3226
                     /locus_tag="AC16CY_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3355056.1"
                     /translation="MIHLDTSVLIDALTGPRRSLPALRQVVADGIRLGVSALVLYEWR
                     RGPRTDTELAIEAALLGPEVVVAFGEVEARMAADLYRRVSRARGRELDLAIAACALSQ
                     GARVWTLNRGDFLDIPGLELHRSG"
     gene            complement(3253..3489)
                     /locus_tag="AC16CY_14505"
     CDS             complement(3253..3489)
                     /locus_tag="AC16CY_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6755 family protein"
                     /protein_id="HIR3355057.1"
                     /translation="MSIHRPFTREQRSTIVLGMLAFVLIIVILQLWLLTATMNAHLGG
                     DQSVVWPAALASLFCLLLNLGLLRYLGNLERTRR"
     gene            complement(3491..4138)
                     /locus_tag="AC16CY_14510"
     CDS             complement(3491..4138)
                     /locus_tag="AC16CY_14510"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR3355058.1"
                     /translation="MNDPRKPTTASIDARVNSPAREGLTGTAPASVYTPAADREQITT
                     PPDFSPVDLQPAWRRDFPVDWPEDRYVERREFTKFMVLTSFALFAGQMWIAAKSWWQG
                     RQGAAPGMRIGSLDDIPVGGTLVFRYPEADDVCVLVRLDERQLVAYSQECTHLSCPVI
                     PRPERGVLHCPCHDGVFDLRSGRAIAGPPSRPLPRILLDVRRRDIYATGVEPGAA"
     gene            complement(4135..4833)
                     /locus_tag="AC16CY_14515"
     CDS             complement(4135..4833)
                     /locus_tag="AC16CY_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3355059.1"
                     /translation="MAKPDDLHFFIDPNRCIGCQACLQACTECDTHRGESMIHLEYVD
                     RAHSAQTVPVVCMHCEQPTCAEVCPADAIKRTGDGIVQSARKPRCIACGNCVVACPFG
                     VPELYADRQIMMKCDMCYDRTSVGKKPMCATVCPSQALFFGTLEQIELLRPLSMPVNR
                     FQFGQQTITTQVFVMMPREIAVRAPLVDVTSAMDERPRSRSVSLRVVPADAGATARAD
                     EDASDPYADTGTWP"
     gene            complement(4847..>6368)
                     /locus_tag="AC16CY_14520"
     CDS             complement(4847..>6368)
                     /locus_tag="AC16CY_14520"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR3355060.1"
                     /translation="RDAALFAGVLRIMIERDWLDHAFIDAHTTGFDQVADYCRQWTLE
                     HTSDVTGVPRNAIAQAAEWWGTARTSFLFHARGIEHHSNGVQNSLGTINLVLASGRIG
                     KPKCGYGTIVGQANGQGGREHGQKCDQLPGWRDIANPEHRRYIAGVWGIDEADLPGPG
                     VDAYEMFRKIDAGEIKALLSICFNPKVSLPDNAFITRCLDKLEFFAAIDFFLNDTAWH
                     ADIVLPGSLHEEDEGTVTQVEGRIIKVNKAVRCPGEAREDWRIIQDIARTLGRPAGFT
                     FAGPRAIFEELRVASKGGVADYSGVTYEKVEAQMGVFWPCYSEDPRTGAPIADHPGTP
                     RLFEPGSYNPVARGTGPFYFPDGRARFNVADYRPPVDDASEEYPVFLSTGRVVSQFLS
                     GTQTRRIGPLVKQYAEPRVEMHPRLAARLGIADGDWTTVETRRGSMTLRAMVAATIRP
                     DTVFIPYHWPGKKSPNQLTIAAQDPISKIPQYKVCGCRVRKADGPPEYAATHEPQQ"
BASE COUNT         1104 a         2053 c         2129 g         1082 t
ORIGIN      
        1 gtcaatctcc gctgcgagcg gactcggctc gaagaatccg cgaagcacga cgaacccgaa
       61 cgtccgaaat gcatcggccg attgacgtgt cagattgaag gtcaaatcag aagatactcc
      121 gactggaata gcaccgtcgt cggtagctga aaccaacgcc ggccgttccg gcggcacgtg
      181 tggggaccag aactccaagg ctggcgagat gatagcagtg acgccgcaat cagaagcgat
      241 agccgcgcgg gatggtcggc ttgaccgctc ggtggtgcgg gcgtactctg tgcccgagag
      301 gaagcgggat gcgatatcga ctccgtagtc tgttcgtcgc cgtggtcatc gcagtaactg
      361 cggccgccat gcaggcgcat cactcggtcc cagggcaatt cgacatgtcc aagccgatga
      421 cgctcaaggg cgtcatcacg aaagtggact ggatcaaccc gcacatctac gtccatctca
      481 acgtcaagga agcggccggc acgaccacca cctgggcgct cgccacgctg ccaacggcga
      541 tgatgcggcg ggcgggcctg accagggaat cgcttcaagg caagcccggt gaagaggtga
      601 cgatcgatgc gttgccggcg cgcgatggaa cgaagcacct tgcctggatc aacaagatca
      661 cgtacgcgga cggacacagt tttgcgccaa ccggccagta gagcgcgggc ttcagcccgc
      721 gcgaacagga aagtcatgac ccgaatcctt gtttcaagca tcgtgagtct gtcggtgctc
      781 gccgctgccg ggacgtcatt cgcgcaggtc gcggctcgag cacccgatcg cggaaccgtc
      841 aagcagctgc agacgctctc ggccaagccg acgcccaggc tggccgacgg ccggaccgac
      901 ttcaacggca cctgggatca cctgggtggt atcgagttcg tacggccggt gaagaaggcg
      961 gacggatcga tttgcttgat cggctgcggt cccgccccgg ctggtgcgcc gccggcgccg
     1021 ttcccgcccg tgcagaccga cttcccgaaa tacaagccgg aattccaggc gaaggtcgcc
     1081 gatctcagca agcggcaggt gcaggtcgat accaacctga agtgccaggc gcccggcgtc
     1141 ccgcgcatcg gcccgccggc caagatcgta cagagcgctc gcgaggtcgt cttcctctat
     1201 gacgatgtga acggagcgtt cttccggatc gtcccgatcg acggacggcc gcaccggaag
     1261 gacgtaccgc ccagttatct gggcgacgcc gtcggccgca tcgagggaga caccctgatc
     1321 gtcgagacag tcggcatgaa cgacgagacc tggctcactg acaacggagc gttccacacg
     1381 aagaacctga gggtgacgga acgcttccgc cgcattggcg acaccatcga atggcgtgcc
     1441 acggtgcacg acccggaggt gctcgtcgag ccgtggtcac tcaagccgca ggtgatgtgg
     1501 atcaccgaca tcgagatcga agagcctgcc cgatgcgagg atcgcgacgt gcctcacatc
     1561 gttgacggat cgcatcacga caaccctcgg tgatgaaaac gtcgtgcgcc agggcagcga
     1621 gctatcagga gttcgccgcc ctgctcgcca gcaccgagac aaccgccgat ctcttcgatc
     1681 tcttcctcgg ccacctcgat ctgatcgagc aggccgaccc gcacctcgtg attggcggcc
     1741 aaacatcgag atcctggcgg cagtttgctc tcggctcgat agcaagactg gccagattga
     1801 tggtggcgag gcgtcaaccg gtcgatgcca aggccaacta cgagcggcgc tggtcgcacc
     1861 atggcgccaa gagccacagg aagcggctgc tcaagccgcg agactatctg atcggcgatc
     1921 gcatccacac gattagtgaa ggcacgttct cggtgctgct cacgcagacg ctcatcacgc
     1981 tcttcaagca gttcattcga caggacgatg gcgtggtcgt ggaactcggt tgcggaaacg
     2041 ggcgcaatct gttcgtgatc cgcagcctct ttccgtccgt cgagtgcatc ggcgtcgata
     2101 tctcgaatgc cggtcccgag tttgcctcgg ccatggggca gcagctcgac gccgacggca
     2161 tgacgtttct cggcgacatc gatatcaccg acggcgcgct catgtcccgg gtcggcgcga
     2221 tgctccgggg caggtccgtc gtggtgatca ccatcggatg cctcgaggtg gtgccggaaa
     2281 cggcgcgggc gatcgaacag attctcgcgc tgaagccgcg ctgcgtgatc cacttcgagc
     2341 ccgtgctcga gctccacaca ggtgtctttc ccagccgtga ctacttcgcc cgccgccacc
     2401 tgatcgcggg gggctacatg acgtcgctcc tcacgatcct cgagcggcac gatcggatcg
     2461 agatcgtgac acgccggcgt gcaggccttg gaaaggcgct gctggagtat tccgtgctcg
     2521 tgtggaagcc gaagtgagga ctgccatact atatatggta gtatggtatc gatatggtca
     2581 aggtcacctt tacgctcgac gatgagaccg tctcgacgct caggaaaacc gcggagcggc
     2641 tggccaggcc gcagagcgcg gtcgtgaggg aggcgatcca cgaatacggc gcccgtgcgg
     2701 gccggctcag cgaggacgag cgccgccgca tgctgcggtc gatcgatgcc gtgctcgcgc
     2761 agccgccgac acggaccatc gccgccgtcg aacgcgagct gcgggagctg cgcgccgcgc
     2821 gccgtggagg cggccgtcgc tccgcatgat tcacctcgac acatcggtcc tcatcgacgc
     2881 gctcacgggt ccgcggcgat cgttgcccgc gctgcggcag gtggttgccg atggcatccg
     2941 cctcggtgtg tccgcgctcg tgctgtacga gtggcgacgc ggcccgcgaa cggacacgga
     3001 gctggcgatc gaggccgcgc tcctcgggcc ggaggtggtc gtcgctttcg gggaggtcga
     3061 ggcgcgaatg gctgcggatc tgtatcgacg ggtgagtcgt gcgcgagggc gtgagctcga
     3121 cctcgcgatc gccgcgtgcg cgctgagcca gggcgcccgc gtgtggacgc tcaaccgggg
     3181 agattttctg gacattcccg gactcgagct gcacaggtcc ggctgaagcc cgacacgacg
     3241 tcgaaccgtg attcagcggc gtgtgcgttc caggttgccg agataccgca gcagccccag
     3301 gttcagcaac agacagaaca ggctcgccag cgccgcgggc cagaccaccg actggtcccc
     3361 gcccaggtgt gcgttcatcg tcgcagtcag cagccagagc tgcaggatca cgatgatcag
     3421 cacgaaagcg agcatgccca ggacgatcgt cgaccgctgc tcgcgtgtga agggacggtg
     3481 gatggacatg tcaggcggcg cccggctcga cgccggtggc gtagatgtcg cgtcgtcgca
     3541 cgtccagcag gatgcgtgga agcgggcgcg acggcgggcc cgcgatcgcc cggcccgagc
     3601 ggagatcgaa cacgccgtca tgacaggggc agtggaggac gccgcgctcc ggcctcggga
     3661 tgaccgggca ggacaagtgc gtacactcct ggctataggc gacgagctga cgttcgtcga
     3721 gccggacgag cacgcagaca tcgtcagcct cggggtagcg gaagacaagt gtgccgccga
     3781 caggaatgtc atcgagcgag ccgattcgca tccccggagc cgcgccctgt cgcccctgcc
     3841 accagctctt tgccgcaatc cacatctgcc cggcgaagag cgcgaagctg gtgagcacca
     3901 tgaacttcgt gaactcgcgc cgctcgacgt agcgatcctc gggccagtcg acggggaagt
     3961 cccgccgcca ggcgggctgt aggtcgaccg gcgagaagtc cgggggagtc gtgatctgtt
     4021 cccgatcggc tgccggcgtg tacacgctgg ctggcgccgt gccggtcaac ccctcgcgcg
     4081 cgggcgagtt cacgcgagcg tcgatcgacg ctgtcgtcgg tttccgcggg tcgttcatgg
     4141 ccaggttccc gtgtcggcgt acggatcgga ggcatcttcg tcggcgcgcg ccgtggcccc
     4201 ggcgtcggcc ggcaccactc tcagcgacac cgagcggctg cgcggccgct cgtccatcgc
     4261 ggacgtcacg tccacgagcg gcgcgcgtac cgcgatttcc cgaggcatca tcacgaacac
     4321 ctgcgtcgtg atcgtctgct ggccgaactg aaaccggttg accggcatcg acagcggccg
     4381 cagcagctcg atttgctcga gcgtcccgaa gaagagcgcc tggcttggac agaccgtggc
     4441 gcacatcggc ttcttcccca cgctcgttcg gtcgtagcac atgtcgcact tcatcatgat
     4501 ctgccggtcg gcgtagagct cgggcacgcc gaacgggcag gcgacgacgc agttgccgca
     4561 ggcgatgcag cggggcttgc gagccgactg cacgatgccg tcgccggtgc gcttgatcgc
     4621 gtcggccggg cagacctcgg cgcacgtcgg ctgctcgcag tgcatgcaca cgaccggcac
     4681 cgtctgggcg ctgtgcgctc gatcgacgta ctcgaggtga atcatcgact cgcctcgatg
     4741 cgtgtcgcac tcggtgcacg cctgcaaaca cgcctggcag ccgatgcacc ggttcgggtc
     4801 gatgaagaag tggaggtcgt cgggcttggc catgcgtatg cacccgctac tgctgcggct
     4861 cgtgagtggc cgcgtactcg ggtggaccat cggcctttcg cacgcggcat ccgcacacct
     4921 tgtattgcgg gatcttgctg atgggatcct gcgcggcgat ggtcagctgg ttggggctct
     4981 tttttccggg ccagtgatag gggatgaaca ccgtgtcggg cctgatggtc gccgccacca
     5041 tggcgcgcag cgtcatcgag ccgcgccggg tctcgaccgt tgtccagtcg ccgtcggcga
     5101 tgcccagccg tgcggcgagc ctcgggtgca tctcgactcg cggctcggcg tactgcttga
     5161 cgagcggacc gatccgccgg gtctgcgtgc ccgacagaaa ctggctcacc acccggccgg
     5221 tcgacaggaa caccgggtac tcttcgctcg catcgtcgac cggcggacga tagtcggcga
     5281 cgttgaagcg tgcgcgtcca tcggggaagt agaacggacc ggtgcccctg gccaccgggt
     5341 tgtagctgcc cggctcgaag agccgcggcg tgcccgggtg atcggcgatc ggcgcgcccg
     5401 tgcgcggatc ctcgctgtag cacggccaga agacgcccat ctgcgcctcc actttctcgt
     5461 aggtcacgcc cgagtagtcg gcgacgccac ctttgctcgc cacgcgcagc tcctcgaaga
     5521 tcgcccgcgg gccagcgaag gtgaatcccg ccggccggcc cagcgtccgt gcgatgtcct
     5581 ggatgatgcg ccagtcctcg cgggcttcac cgggacatcg gacggccttg ttgaccttga
     5641 tgatgcgccc ctccacctgc gtgaccgtcc cttcgtcctc ctcgtgcagg cttcccggga
     5701 ggacgatgtc cgcgtgccac gcggtgtcgt tcaggaagaa gtcgatcgcg gcgaagaatt
     5761 ccagcttgtc gaggcatcgg gtgatgaagg cgttgtccgg gagcgagacc ttcgggttga
     5821 agcagatcga gagcagggcc ttgatctcgc cggcgtcgat cttgcggaac atttcgtacg
     5881 cgtcgacgcc cggtcccggc agatccgcct cgtcgattcc ccagacgccg gcgatgtatc
     5941 ggcgatgttc ggggttggcg atgtcgcgcc aaccgggcag ctggtcgcac ttctgaccgt
     6001 gttcccggcc gccctggccg ttggcttggc cgacgatggt gccgtagccg cacttcggtt
     6061 tgccgatgcg gccagaggcg agcacgaggt tgatggtacc gagggaattc tgcacgccgt
     6121 tggagtggtg ctcgatgccg cgcgcgtgaa acaggaagct cgtgcgggcc gttccccacc
     6181 attcagcggc ttgcgcgatc gcattcctgg gaacgccggt cacgtccgag gtgtgctcga
     6241 gcgtccactg ccggcagtag tcggcaacct ggtcgaaccc cgtcgtgtgc gcgtcgatga
     6301 aagcgtgatc gagccagtcc cgctcgatca tgatcctgag cacgcccgcg aagagcgcag
     6361 cgtcgcgt
//
LOCUS       DBNGSH010000626         3232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3798782, whole genome shotgun sequence.
ACCESSION   DBNGSH010000626 DBNGSH010000000
VERSION     DBNGSH010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3798782"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC16CY_14525"
     CDS             complement(<1..512)
                     /locus_tag="AC16CY_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355061.1"
                     /translation="MRSSLSAVLAIVVWLVPSPVLGQDVDFSGEWAPLYHEDGPERLP
                     GPEIGDYTGLPLNDAARLRADSYDADRISVVTEYQCRQHGGDYSMRGLANLRITRELD
                     PRTQQAVAFHTRIGFHDMERTIYLDGRPHPPAEAAHTWQGFSTGVWEGNMLTITTTHL
                     KANYLRRNGMP"
     gene            581..1162
                     /gene="efp"
                     /locus_tag="AC16CY_14530"
     CDS             581..1162
                     /gene="efp"
                     /locus_tag="AC16CY_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3355062.1"
                     /translation="MPSLIEAIDIKRKTFFEFEGAPYVCLDVEVSRPTARGGQTLVRV
                     KMRNLLTRAVFDKTFKAGEKFGEPDLVQVTASFLYSDGEGYHFMDQDSFETLTLAAAT
                     VGDDRELLVDNVEVQIHKYNGNPIGIQFPPHVELTVSYTEPGVRGDTASGGVTKAATL
                     ETGLQIRVPLFIKEGEKVKVHTETREFAGRAGG"
     gene            1181..>3232
                     /locus_tag="AC16CY_14535"
     CDS             1181..>3232
                     /locus_tag="AC16CY_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355063.1"
                     /translation="MALTPGTKVGPYEVLAQIGAGGMGEVYRARDTRLDRVVALKALP
                     SGTTTPQALERFEREAKAIAALNHPNICSVYDVGTAPAPYLVMELLEGESLHERLTRG
                     PLEVASLVDVALALADAIAAAHARGTVHRDLKPANVVLTPHGPKILDFGLARVVEGTM
                     SPGVDATASPTMPAVAPLTDAGVTVGTVAYMSPEQLRGEALDARTDIFSMGLVLYEMA
                     TGQRAFVGGTSAVVSAAILHERPRAPRTLRPDLPASLEQAILTALEKDRDTRTQAATE
                     LRAELKRIKRELGGALALPATAPASGSVVTGSSASREPAASTVAASPSSSSDAQVIAS
                     VIGRHRKALAGVAAVVVLLLGGVTWLLTRQTGTGTVRERAALSIADLEPLVQLTTSGT
                     ARAPAISPHGDMVAYVESGGRADSLRVRQVATGSNDPGVEIVPPTPGISLAGANFTPN
                     GEYVDYLRRITPTAVELWRIPKLGGRQPRRLAENVISGVGWSPDGREMAYVRAPSPGR
                     TELVIAEDDASAPQVVAARDAPLDFYFASPPGGPAWSPDGSAIALVGRDGGSPRTGQI
                     VVVDKATRTEQTVATGPPGFAFALAWLDGKTLLLSMLDKISALAQLWTLSYPEGRFTR
                     LTNDTSQYVGLSVTADRNAWLTSKLEVSFGVWTNKSGGAPSQIVSMRPTKASIGFGLR
                     WI"
BASE COUNT          550 a         1068 c         1100 g          514 t
ORIGIN      
        1 ggcatgccgt tgcgacgcag gtagttcgcc ttgaggtgcg tggtggtgat ggtcagcatg
       61 tttccttccc acaccccggt agagaagccc tgccacgtgt gcgctgcctc ggcgggcgga
      121 tgcggcctgc cgtccagata gatcgtccgt tccatgtcgt ggaagccgat gcgggtgtgg
      181 aaggcgacgg cctgctgagt ccgcgggtcg agttcccgcg tgatgcgaag gttcgccagg
      241 ccgcgcatcg agtagtcgcc gccgtgctgc cggcattggt actccgtgac gacggagatg
      301 cggtcggcgt cgtagctgtc ggcgcgcagc cgcgccgcat cgttgagagg caggccggtg
      361 tagtcgccaa tctcagggcc gggcagccgc tcgggaccgt cctcgtgata gaggggcgcc
      421 cactcaccgc tgaagtcgac gtcttggccg agcaccgggg atgggacgag ccacaccact
      481 atcgccagaa cggccgacag cgacgatctc accccgccta cgcgcatcgt caaccctacg
      541 cagcagacta tacccgcacc cgcgatagac tcgtacccgc atgcccagcc tgattgaagc
      601 catcgacatc aagcggaaga cgttcttcga gttcgagggt gctccctacg tctgcctgga
      661 cgtcgaagtg tcgcggccga cggcgcgcgg aggccagacg ctcgtccgcg tgaagatgcg
      721 aaacctgctg acacgggccg tcttcgacaa gaccttcaag gcgggcgaga agttcggcga
      781 gcctgatctg gtccaggtga cggcgtcgtt cttgtattca gacggcgagg gttaccactt
      841 catggaccag gacagcttcg agacgctcac gctggccgcg gccaccgtgg gcgacgatcg
      901 cgagctgctc gtggacaacg tggaggtcca gattcacaag tacaacggca atccgatcgg
      961 catccagttc ccgccgcacg tcgagctgac cgtgtcctac accgagcccg gcgtgcgagg
     1021 cgacaccgcc tcgggcggtg tcacgaaggc cgcgacgctc gagaccggcc tgcagatccg
     1081 cgtgcccctc ttcatcaagg aaggggagaa ggtgaaggta cacaccgaga cgcgcgagtt
     1141 tgcgggaagg gcgggtggct gaggatagaa tcgggcgatc gtggcgctga cgcccggcac
     1201 gaaggtcggt ccctacgagg tcctcgctca gataggcgca ggcgggatgg gcgaggtcta
     1261 ccgggcgcgc gacacgcgcc tcgatcgcgt tgtcgccctg aaggcgcttc cttccgggac
     1321 cacaacgccc caggcgctcg aacggttcga gcgcgaggcc aaggccatcg cggcgctcaa
     1381 ccaccccaac atctgctccg tttacgacgt tggcactgcg cccgcgccgt acctggtcat
     1441 ggagctgctc gagggcgagt cgctgcacga gcgtctcacg cgcggcccgc tcgaagtcgc
     1501 ttcgctcgtc gacgtggctc tcgccctcgc tgacgcgatc gccgccgcgc acgcccgcgg
     1561 cactgtccac cgcgacttga agccggccaa cgtcgtcctg acgccgcacg gcccgaagat
     1621 cctggacttc ggcctggcgc gtgtcgtcga aggcacgatg tcgcctggcg tcgatgcgac
     1681 cgctagtccg acgatgccgg ccgtcgcccc gctcacggac gcaggcgtga ccgtcgggac
     1741 ggtggcgtac atgtctcccg agcaattgcg cggcgaggcg ctcgatgcgc gcacagatat
     1801 tttctcgatg gggctcgtgc tctacgagat ggcgacgggc caacgcgctt tcgtcggcgg
     1861 gacgagcgct gtcgtctctg ccgcgatcct gcacgagcgg ccgcgggcac cgcggacgct
     1921 gcgccccgat cttcctgcga gcctcgagca ggcaatcctc acggccctgg agaaggaccg
     1981 cgacacgcga acccaggccg ccacggagct gcgcgccgag ttgaagcgaa tcaagcgaga
     2041 gttgggagga gcgctcgcgc tgcccgctac ggcgcccgcg tcggggagtg tagtcactgg
     2101 cagctctgcg agccgcgagc cggctgcgag cacagtcgcg gcgtctccgt cgagctcttc
     2161 ggatgcccag gtcatcgcca gcgtgatcgg ccggcatcgc aaggcgctgg caggggtggc
     2221 tgcggtcgtg gtgctgctgc tcggcggtgt cacttggctc ctgacgcggc agaccggcac
     2281 cggtacggtt cgcgagaggg cagcactttc catcgcggac ctggagcccc tcgtccagct
     2341 cacgacgagc ggcaccgcaa gggcgccggc catctcccct cacggcgaca tggtcgccta
     2401 tgtcgagtcg gggggcagag ccgacagtct cagggtgagg caggtggcga ccgggagcaa
     2461 cgacccagga gtcgagatcg tgccgcccac gcccggcatc agtctcgccg gcgcgaactt
     2521 cacgccgaac ggcgaatacg tggactacct gcggaggatc acgccgaccg ctgtcgaact
     2581 atggcgaatc ccgaagctcg ggggtcgtca accgcggcgg ctggccgaga acgtcatcag
     2641 tggagtgggc tggtcgcccg acggacgcga gatggcgtac gtccgggcgc cctctccggg
     2701 gcgcaccgag ctcgtgatcg ccgaagacga tgccagcgcg ccgcaagttg tggccgcgcg
     2761 agacgcgccg ctggactttt acttcgctag tcctccgggt ggtccagcct ggtcacccga
     2821 cgggtccgcc atcgcactcg taggcagaga cgggggatct cctcggaccg ggcagatcgt
     2881 ggtcgtcgac aaggccacac gcaccgagca aacagtggcc accggcccgc cggggttcgc
     2941 ctttgcactc gcctggctcg acggcaagac gcttcttctc agcatgctcg acaagatcag
     3001 cgcgctggct cagctctgga ccctgtcgta cccggaaggc aggttcacgc gactcaccaa
     3061 cgacacgagt cagtacgttg ggctcagcgt gacggccgat cgaaacgcat ggctgacatc
     3121 aaagctggaa gtgtcattcg gcgtctggac gaacaagagt ggcggcgcac cgagccagat
     3181 cgtgtcgatg aggcccacaa aagccagcat tggcttcggt ctgcggtgga tc
//
LOCUS       DBNGSH010000627         4498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_233120, whole genome shotgun sequence.
ACCESSION   DBNGSH010000627 DBNGSH010000000
VERSION     DBNGSH010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_233120"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(568..1521)
                     /locus_tag="AC16CY_14540"
     CDS             complement(568..1521)
                     /locus_tag="AC16CY_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355064.1"
                     /translation="MGQAGHTRRQVLKAAMGGAAGVLLGAPAFAAPRLRRGKPVQTAS
                     DTAGTLRLSDDLFVITIPGEANVVAHIGPDGVVLVDGASARASDALMKAVAALPGGGP
                     VRTIFNTHWHPEQTGSNERLGKAGNTIIAHENTRLWLTTDVTWPWSGQRFTRLPKAAQ
                     PNKTFYTTGTLDSGVRYGYIPDAAHTDGDLYVYFPKQNVLAVGDVVSGQGWPVVDYAT
                     GGWIGGMVGGLQRLLSLANDETRIVPGRGPVLGLKDLKSQAQMYGTIYERLTQLLNKG
                     RGPGEAVAAQPTKEFDAQMGNGDEFVRRAFESLWAYLSPDA"
     gene            complement(1558..1845)
                     /locus_tag="AC16CY_14545"
     CDS             complement(1558..1845)
                     /locus_tag="AC16CY_14545"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3355065.1"
                     /translation="MSGGEWAVRWRHDGQLARAVVSALERSAVVPEGKIWVAVRDGQV
                     TLKGQVDSDRQRAAAVTLAHDLTGVRRVVDSITVAERALSAAGAATALHSS"
     gene            complement(2156..>4498)
                     /locus_tag="AC16CY_14550"
     CDS             complement(2156..>4498)
                     /locus_tag="AC16CY_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HIR3355066.1"
                     /translation="PIVVRSRGSEALSVGRVALVGDHQGDFRIVSNGCTGTELRDADA
                     ECTIEVEFAPVAVGVRSARLQLADNSNQSPRFVALSGEARPVPVPRPRLSPDRLEFSQ
                     QVVGQPGTARRVTVTNEGSATLSIQRVLLEGRHPRDFTITGTDCTGANLPPQGTCTVD
                     VGFGPTAAGRRAGSLTVVHNAGDAGRNVDLNGTAEARPMADVSPPRLEFDEQPLEKSS
                     RQRSVRIANAGGGVLQVGRITLEGVNRRDFSLENRCTQSGRGLRRGESCEVLVQFVPD
                     AAGARSAVMVVTQTDGIRHEVALSGVGVGRGVLAVLPESLEFGVQELKTSREQQVTVT
                     NAGSAVLAVASIAVDKSGAREYAVVDPQKCANQKLDPGGKCTFGVRFTPAGVDERPAS
                     ISVADATGSRISVALSGAGASPRPSPVSQLSFKPDRLDFGELVVPSRQFDKSAGIASR
                     AVTLTNVGNVDVVVADVLVEPAESSREFQIQDKCRGARLAPQQDCTVRVTFRASAPGP
                     RQASLVFLNNAAKNPHRVALFGFGREATIPNEESKRCSDFVADIGLDVVRHTKPYVAD
                     VRITGLARNLGPSVLSEPGGMIWLYEGNRLVREDKLSAVDVGNVVSVVYERQWDSASP
                     SEGEFPPTYRVVVKGPTDCSGNNNEVRRSGADLNTKIRETMPPSTSGVKPDPLRPIPG
                     TRLPLPVIGQFIAVDKPGQPRQLCYAVKGATSARIEPGVGPVKPLPNQTCVRVSPVRT
                     TEYVLMADNADGAGVKRSVTFVVTSPQRLQQPPPPAVAKE"
BASE COUNT          766 a         1507 c         1421 g          804 t
ORIGIN      
        1 gggacgcggg ggccgtctgc tgaggcgccg tgtacaccac ggaatggacg gggcctggaa
       61 tcagattcca gccgaagaac cacgccagga ggaagaatgc ggcgacgatg acgagccccg
      121 ccagcaatcg ggctctccta cgttccgcgg ccaacgcctc cgtgctcgct cgcgatctcc
      181 tccagcgctc ggtcgtgacc acaacagcca tcgtcgaatc caatctaacc ccagggctcg
      241 cttgtgttga tcccattagc caggtcgttt ccgatcggaa tcggccggat ttgttccccg
      301 ggccggcgcc gaaggcctca acacaagcga gctgtggagt aagtcccttg aatcgagcac
      361 gagatccgtc gttcgccctt ctccgttctc gcttctctgt tcgtgttcag gttcggagag
      421 agatgcggcc ggcatcgcca agccccccgg agggaacgcc gaaccccgaa cacgccgaac
      481 ctcgaaccga acgtgaacac gaaccgagaa gtcagaacca agaagcgtga acacgggatc
      541 tctggcccca atcacgtgac tcgacgttca tgcgtcaggc gacaggtagg cccagaggct
      601 ctcgaaggcg cgccgaacga actcgtcgcc gttgcccatc tgcgcgtcga actccttggt
      661 cggctgcgcg gccacggcct cacccggtcc gcgccctttg ttcagcagct gcgtcagccg
      721 ttcgtagatc gtgccataca tctgggcctg tgacttgaga tctttcaggc cgagcacggg
      781 cccgcgcccg ggcacgattc gcgtctcgtc attggccagc gacagcaatc gttgcagccc
      841 tccgaccatg ccgccaatcc agcccccggt cgcgtagtcc acgaccggcc agccctggcc
      901 cgacacgacg tcgcccaccg cgagcacgtt ctgcttcggg aagtacacgt agagatcgcc
      961 gtcggtgtgc gccgcgtcgg gaatgtagcc gtagcggacg cccgagtcga gggtgcccgt
     1021 cgtgtagaac gtcttgttgg gctgcgcggc cttcggcagg cgcgtgaacc gctgcccgct
     1081 ccacggccac gtgacgtcgg tcgtcagcca gaggcgcgtg ttctcgtgcg cgatgatcgt
     1141 gttgccggcc ttgccgagcc gctcgttcga gccggtctgt tcggggtgcc aatgcgtgtt
     1201 gaagatcgtc cgcaccggac cgccgcctgg cagcgcggcg actgctttca tcagcgcatc
     1261 cgacgcgcgg gccgatgcgc catcgacgag cacgacgccg tccgggccga tgtgcgcgac
     1321 gacgttggct tctccaggga tcgtgatgac aaacagatcg tcactcagcc gcagcgtgcc
     1381 tgcggtgtcg gaagcagtct ggacgggctt gccccgccga agccgcggag cggcgaaggc
     1441 gggtgccccc agaaggactc cggcggcgcc gcccatcgcg gccttcagaa cctgtcgtcg
     1501 cgtgtgaccg gcctgaccca cggcaggtcc agtgtagacc ggtttcttag gtcgtcctca
     1561 actggaatga agagctgtcg cggctcctgc cgcgctgaga gcccgctcgg cgaccgtgat
     1621 cgaatcgacc acgcggcgaa cgcccgtcag atcgtgcgcg agcgtcaccg ctgccgcgcg
     1681 ctgacgatcc gagtcaacct gccccttcag cgtcacctgg ccgtccctga cggcaaccca
     1741 gatcttgcct tccggaacga ccgcgctcct ttcgagcgcc gatacgacgg cgcgcgcgag
     1801 ctggccgtca tggcgccacc gaactgccca ttccccaccc gacattgccc acccccttac
     1861 gagttcgatc ggaacccgca cgttttacgt gcgatcgggc tgtatgacca tgggcggagt
     1921 atgagggacg gccgattgtt ccccgagcgc ccaagctcct ttcaacgcaa gaagttccgg
     1981 gacgggatgg gtgaacgcga ggcgccgtct tctgtcggct gggcctgttt gcggatccat
     2041 ccggcagcgt cgagttctcg aacgcgctcc acggctgcac cagctttgtc cggaggcgcc
     2101 tcctgtgaca cgcgctcctc gagctcgcgg atgagccgcc gcagttctca tcgtgtcact
     2161 ccttggcgac ggccgggggg ggaggctgtt ggagacgttg cggtgatgtc acgacgaagg
     2221 tcacgctgcg tttgacaccg gccccgtcgg cgttgtccgc catcaagacg tactcagtcg
     2281 tcctcacggg cgaaacgcgc acgcaggtct gattcggcaa cggcttcacg gggcccacgc
     2341 cgggctcgat gcgcgcgctg gtggcgccct tgaccgcata gcagagctga cgcggctgcc
     2401 ctggcttgtc tacagcgatg aattggccga tcactggcag aggcaatctg gtccctggta
     2461 ttggtcgcaa cggatcgggt ttgacaccgg atgtggacgg aggcatggtc tcgcgaatct
     2521 tggtgttgag gtcggcaccg cttctccgga cttcgttgtt gttgccgctg caatcagtcg
     2581 gccccttgac cacgacacga tacgtcggtg ggaactcgcc ttcgctgggc gacgcactgt
     2641 cccactgacg ctcatagaca acggatacga cgttgcccac gtcaacggca gacagcttgt
     2701 cttcgcgaac gagcctgttg ccttcataca gccagatcat gcccccgggt tcagagagga
     2761 cgctggggcc gaggtttctc gcaagtcccg tgatccgcac gtcggccacg tacggcttcg
     2821 tgtgtcgtac gacgtcgagg ccgatgtcgg cgacgaagtc ggagcacctt ttcgactcct
     2881 cgttgggaat cgtcgcctcg cgaccgaagc cgaacagcgc cacgcgatga gggttcttcg
     2941 ctgcgttgtt caggaagacg agcgatgctt gccgcggacc tggagccgaa gcgcggaatg
     3001 tcactctcac ggtgcagtcc tgttgaggcg ccagccgagc gccgcggcac ttgtcctgta
     3061 tctggaactc tcgtgacgac tccgccggtt cgacgagaac atcagcgacc acgacgtcga
     3121 cgttcccgac attggtcagc gtgactgccc gcgacgcaat gccagcggac ttgtcgaact
     3181 gtctcgaggg gacgaccaac tcgccgaaat caaggcgatc gggcttgaac gacagctgac
     3241 tgactggcga gggtcgcggc gaagcgcctg cgccgctgag tgccacactg attcgtgaac
     3301 ccgttgcatc agccaccgag atcgacgccg gccgttcatc gacgcctgcc ggggtgaatc
     3361 gtacgccgaa ggtgcacttc ccgccgggat cgagcttctg gttggcgcac ttctggggat
     3421 cgacgacggc gtactcgcgc gcaccggatt tatcaacagc aatagaagca acggcgagaa
     3481 cggccgagcc cgcatttgtc accgtcacct gttgttcgcg actcgtcttc aactcctgga
     3541 cgccgaactc gagcgattcc ggaaggacgg cgagcacgcc gcggccgacg ccaacacctg
     3601 acagtgcaac ttcgtgtcga atcccgtcag tctgtgtgac gaccatcacg gccgatctgg
     3661 cgccggcggc atccggcacg aactgcacca gcacctcgca cgattcgcca cgcctgagac
     3721 cacggcccga ttgtgtgcag cgattctcca gggagaaatc gcgacggttg acgccctcga
     3781 gcgtaattcg cccgacctgc agcacgccgc cgccggcgtt tgcaatcctc acgctgcgtt
     3841 ggcggctgga cttctcgagc ggctgttcgt cgaattcgag ccgagggggc gacacgtccg
     3901 ccatcggccg cgcctcggca gtgccattca gatccacgtt ccggccggcg tcgccggcgt
     3961 tgtgaacgac cgtcaaggac ccggcgcgcc ggcccgccgc tgttggcccg aaccccacgt
     4021 cgaccgtgca ggtcccctgg ggcgggaggt ttgccccggt gcagtccgtg ccggtgatcg
     4081 tgaaatcacg tggatgccgt ccctccagca ggacgcgttg gatcgaaagt gtggcgctgc
     4141 cctcgttggt gacggtcact cgtcgagccg tgcccggctg cccgacgact tgctgcgaga
     4201 actcgagccg gtccgggctc agcctcggtc tcgggacggg aacggggcgg gcctcaccgg
     4261 acagcgccac gaagcgcgga ctttggttcg agttgtcggc cagttgcaac cttgcgctgc
     4321 ggacgccgac tgccaccgga gcgaattcaa cttcgatcgt gcactccgcg tcggcgtcgc
     4381 gtagttcggt cccggtgcat ccgttcgaga caatccgaaa atctccctga tggtcgccaa
     4441 cgagcgcgac ccgtccaacc gacagggcct ccgagccgcg gctgcggacg acaatcgg
//
LOCUS       DBNGSH010000628         1618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_403224, whole genome shotgun sequence.
ACCESSION   DBNGSH010000628 DBNGSH010000000
VERSION     DBNGSH010000628.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_403224"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..906
                     /locus_tag="AC16CY_14555"
     CDS             49..906
                     /locus_tag="AC16CY_14555"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3355067.1"
                     /translation="MATEPTATRRILLVDDDRGLRFALSAMLKDAGHHVEVAGDGPEA
                     LALLESGRFDIVLLDIGLPSMNGLDVLKRARALDAPPVVVVMTADDTSETLLEAARRQ
                     AYRYLRKPFAPNVIVEVVQDAAASASAAALSIEVVSARPEWLELIAPCALEMADRIRS
                     FVMHLEADLPEPVRESVAQAFRELLTNAIEWGGKLDPERKVRISFVRAKRMLLYRIAD
                     PGEGFDIDRLRHAAISNPADDPIRHLTVREEKGLRPGGFGLAMTQSLVDELIYNEARN
                     EVMFIKYLD"
     gene            919..1287
                     /locus_tag="AC16CY_14560"
     CDS             919..1287
                     /locus_tag="AC16CY_14560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3355068.1"
                     /translation="MTLRMRIDERRVEDVTILTLKGRLELEEGDVTLRDHVNKLVEEG
                     RVKLVLDMREVTRLDSAGIGMLVSKFLTARRRGGAIKLIHLTDRSGHLMEITRLSTVF
                     EIFQDEDEALRSFGVGLERT"
     gene            1289..>1618
                     /locus_tag="AC16CY_14565"
     CDS             1289..>1618
                     /locus_tag="AC16CY_14565"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3355069.1"
                     /translation="MRALRAPAFAAAFVAAGASVALAQEPDGRSLFAQRCAACHTGEA
                     NSRAPGLDALAVRTPQTIVESLMTGAMRAQGSRMSGAERRAVAVFITGKTIAGDVRGA
                     LTGRCTVP"
BASE COUNT          264 a          553 c          541 g          260 t
ORIGIN      
        1 tcaagaaatg actcccgacc ccattttctc ccgaccccat ttcacgcgat ggccaccgag
       61 cccaccgcta cccggcgcat cctgctcgtc gacgacgatc gtggactgcg gttcgcgctg
      121 tcggcgatgt tgaaagacgc tggccatcac gtcgaggtcg cgggcgacgg cccggaagcg
      181 ctggcgctgc tggagtcggg ccgcttcgac atcgtgctcc tcgacatcgg gctgcccagc
      241 atgaacgggc tcgacgtgct caagcgcgcc cgcgccctgg acgccccgcc cgtcgtcgtc
      301 gtgatgaccg cggacgacac ctccgagacg ctgctcgaag cggcacggcg gcaggcgtac
      361 cggtacctgc ggaagccgtt cgcgccgaac gtgatcgtcg aggtcgtcca ggatgccgcc
      421 gcgtccgcgt cggcggccgc gctgtcgatc gaggtcgtgt cggccaggcc agagtggctg
      481 gagctcatcg ccccatgcgc cctcgagatg gccgaccgca tccgctcgtt cgtcatgcac
      541 ctcgaggccg atctgcccga gccggtgcgt gagtcggtgg cgcaggcgtt tcgcgagctg
      601 ctcaccaacg ccatcgagtg gggcggcaag ctcgatcccg agcgcaaggt ccggatctcg
      661 ttcgtccgcg cgaagcggat gctgctctat cgcatcgcgg atccgggaga gggcttcgat
      721 atcgaccggc tgcgccatgc cgccatctcg aatcccgccg acgacccgat tcggcatctg
      781 accgtcagag aagagaaggg cctccggccg ggcggcttcg gcctcgcgat gacgcagtcg
      841 ctcgtcgacg agctgattta caacgaggcg cgcaatgagg tcatgttcat caaatacctc
      901 gattgacgga ggcaggtcgt gacgcttcgc atgcgaatcg acgaacggcg cgtcgaggat
      961 gtcaccattc tcacgctgaa gggccgcctc gagctcgagg aaggggacgt cacgctgcgc
     1021 gatcacgtga acaagctcgt cgaggaagga cgcgtcaagc tggtgctcga catgcgggag
     1081 gtcacccgcc tcgacagcgc cggcatcggc atgctcgtca gcaagttcct gaccgcccgg
     1141 cggcggggag gcgcaatcaa gctgatccac ctgaccgatc gcagcggcca cctgatggag
     1201 atcactcgcc tgtccacggt attcgagatc ttccaggacg aagatgaggc cctgcggagc
     1261 tttggcgtcg gattagagag gacctgacgt gcgcgcgctc cgggcgccgg cgtttgcagc
     1321 cgcctttgtc gccgccggcg cgagcgtcgc cctggcgcag gaacccgatg gccggtcgtt
     1381 gttcgcgcag cgctgcgccg cctgtcacac cggcgaggcc aactcacgcg cgcctggtct
     1441 cgacgcgctt gccgtccgga cgccccagac gatcgtcgag agcctcatga ccggcgccat
     1501 gcgcgcgcag gggtcgcgca tgagtggcgc cgagcggcgt gcggtcgccg tgttcatcac
     1561 cgggaagaca atcgcaggcg acgtccgggg cgccctcacc ggacgctgca ccgtgcca
//
LOCUS       DBNGSH010000629         1764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3290610, whole genome shotgun sequence.
ACCESSION   DBNGSH010000629 DBNGSH010000000
VERSION     DBNGSH010000629.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3290610"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="AC16CY_14570"
     CDS             <1..481
                     /locus_tag="AC16CY_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3355070.1"
                     /translation="TRFALAKAPEGWYLKSFAIGGVDVTDAPFDFGSSGATFEGAEIV
                     VSRAGASVTGTVMGDAGKPSAAPSVVVFSVDPGRWFDGSRHVKYARAQGDGSYRIRGL
                     PPGTYLIAAADAADSALAAGGWQDPDVLASLAPLATRLTLTESQAAKTTLRVIRRPR"
     gene            complement(487..1473)
                     /locus_tag="AC16CY_14575"
     CDS             complement(487..1473)
                     /locus_tag="AC16CY_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3355071.1"
                     /translation="MRTSFSVVVVGLAALAASGVIRAAGEPPAWAYAIPPAAPAGAPA
                     AQPAAPDPTPKTLPGSTLSFPLANIRDAFGPADWFPGDHPPMPDVVSKGRRPDVRACG
                     LCHYPNGKGRPENAGVSGLPVSYFIQQMYDFRNDVRKSGEPRKANTNIMIAIAKAMTE
                     EEIKASAEYFGSMKWTPWIRVVEAKVVPKTRIQGGMYLRLEGSETEPIGMRIIETPEN
                     VEHTEVLRDPRSGFIAYAPPGSIKKGESLVTTGGNGRTVQCGVCHGADLKGLGPVPGL
                     AGRSPSYMVRQMYDMQVGARRGVWTDLMKPVVAKLTDEDFVSIAAYLSSRTP"
     gene            1530..>1764
                     /locus_tag="AC16CY_14580"
     CDS             1530..>1764
                     /locus_tag="AC16CY_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355072.1"
                     /translation="MRRLATLIIMCAIATPLTAQWLKEPTRGIPRTKDGKPDLKAPAP
                     RTADGKPDLSGLWRFDAGAYGGNIVADLKPADIQ"
BASE COUNT          269 a          591 c          600 g          304 t
ORIGIN      
        1 gaccaggttt gctctcgcga aagcgcccga ggggtggtac ctgaagtcgt tcgcaatcgg
       61 cggcgtggac gtcacggatg cgccgttcga cttcggatcg agcggggcaa ccttcgaagg
      121 cgcggagatc gtggtgtcgc gcgccggtgc gtcggtgacc ggcaccgtca tgggagacgc
      181 gggcaagccg tcggcggccc cctcggtcgt cgttttttcg gtggatcccg gcaggtggtt
      241 cgacggctcc aggcacgtca agtacgcgcg cgcgcagggc gacggctcct acaggatccg
      301 cggccttccg cccggcacct atctgatcgc agcggccgat gcggccgata gcgcgctggc
      361 tgccggcggc tggcaggacc cggacgtgct cgcgagcctc gcgcccctgg cgacacggct
      421 gacgctcacc gaaagccagg ccgcaaagac cactttgcgc gtaatccgcc gcccgcgctg
      481 attcttctac ggcgttctcg acgagagata cgccgcgatc gacacgaagt cctcgtcggt
      541 gagcttggcc acgaccggct tcatcagatc ggtccacacg ccgcggcgcg cgccgacctg
      601 catgtcgtac atctgccgca ccatgtagct cggcgaccgt cccgcgaggc cgggcaccgg
      661 acccaatccc ttcaggtccg cgccgtggca cacgccgcac tgcaccgtcc tgccgtttcc
      721 gcccgtggtg acgagcgact cgcctttctt gatgctgccg ggaggcgcgt aggcgatgaa
      781 gccggagcgc gggtcgcgaa gcacttcggt gtgctcgacg ttctcgggcg tctcgatgat
      841 gcgcatgccg atcggctcgg tctcgctgcc ttcgaggcgg aggtacatcc cgccctggat
      901 gcgcgtcttc ggcaccacct tggcttcgac gacgcggatc cacggcgtcc acttcatcga
      961 gccgaaatac tcggctgacg ccttgatctc ttcctccgtc atcgccttcg cgatggcgat
     1021 catgatgttc gtgttggctt tccggggctc gccgctcttc cggacgtcgt tcctgaagtc
     1081 gtacatctgc tggatgaagt acgagaccgg gagtcccgac acgccggcgt tctcgggacg
     1141 gcccttgccg ttcgggtagt ggcacagacc gcaggcgcgc acgtctggcc ttcgaccctt
     1201 cgagaccaca tcgggcattg gcgggtgatc gccggggaac cagtcggcag ggccgaacgc
     1261 gtcgcggatg ttcgcgagcg gaaagctgag cgtgctgccc gggagcgttt tcggcgtcgg
     1321 gtcgggagcc gccggttgag cggctggagc gccggccggc gccgcgggcg ggatcgcata
     1381 cgcccatgcc ggaggttcgc ccgccgctcg aatcacgccg ctggcagcga gagccgccag
     1441 acccacgacg accacactga aggatgtgcg catgactggc ctccggttgg gtggtattgt
     1501 gcctcaaggt tgccttggag aaaacgatca tgcgcaggct cgcgacgctg atcattatgt
     1561 gcgccatcgc cactccgctg accgcgcagt ggctgaagga gcccaccagg ggcatcccgc
     1621 gtaccaagga tggaaagccg gatctgaagg cgcccgcgcc tcgcaccgcg gacggcaagc
     1681 cggatctgtc ggggttgtgg cgcttcgacg cgggggcgta cggcggcaac atcgtcgccg
     1741 acctgaagcc agccgacatc cagc
//
LOCUS       DBNGSH010000630         2076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_913743, whole genome shotgun sequence.
ACCESSION   DBNGSH010000630 DBNGSH010000000
VERSION     DBNGSH010000630.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_913743"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="AC16CY_14585"
     CDS             <1..254
                     /locus_tag="AC16CY_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR3355073.1"
                     /translation="RAILSTTRKAMSGQAKLLVVETLVSPPNQPGGKVGDVFMLVRTG
                     GRNRTEQEYRSLLASGGFDTTRVISTGTALSIIEAVPRN"
     gene            302..529
                     /locus_tag="AC16CY_14590"
     CDS             302..529
                     /locus_tag="AC16CY_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HIR3355074.1"
                     /translation="MRTTLDLDEDVLQAAKELAATRGTTAGRVISELARKALSPTRVS
                     RVRNGVPVLPRRPAGAARPTMKLVNTLRDEP"
     gene            519..980
                     /locus_tag="AC16CY_14595"
     CDS             519..980
                     /locus_tag="AC16CY_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HIR3355075.1"
                     /translation="MSRKRPALLDVNLLVALFDADHVHHELAHDWFADQRADGWATCP
                     VTENGLVRVLCNPRYGSPEHAVPAVVDRLRQFRASGHHQFWADEVSLTDNALFKPSFA
                     RGHRQVTDVYLLGLAHRKGGRLVTFDRSIPLGAVAGAVRATLEVVGSVDDD"
     gene            complement(971..2076)
                     /locus_tag="AC16CY_14600"
                     /pseudo
     CDS             complement(971..2076)
                     /locus_tag="AC16CY_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349110.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          357 a          712 c          697 g          310 t
ORIGIN      
        1 ccagggcgat cctgtcgacg acgcgcaagg ccatgtcggg ccaggcgaag ctcctcgtcg
       61 tcgagaccct cgtgagtccc cccaatcagc cgggcggcaa ggtcggtgat gtgttcatgc
      121 tggtccggac cggcggacgc aatcggacgg aacaggaata caggagcctg ctcgcgagcg
      181 gcgggttcga cacgacccgc gtgatctcga ccggaaccgc gctgtcgatc atcgaggcgg
      241 ttccgagaaa ctgaccttca gtctttttga catctgatgc gtgatgcgtg atgatcgaaa
      301 cgtgcgcacc accttggacc tggacgagga cgtcctgcag gcggcaaagg aactggccgc
      361 cacgcgtggc acgacagcgg gacgcgtcat ctcggagctt gctcggaagg cgttgtcgcc
      421 tacgcgtgtg tctcgcgtcc gcaacggcgt cccggttctg ccgcgccggc cggcgggtgc
      481 ggcgcgcccc acgatgaagc tggtgaacac ccttcgggat gagccgtaaa cgtcccgcgc
      541 ttctcgacgt caacctgctc gtcgcgctct tcgacgccga tcacgtgcat cacgaactgg
      601 cccatgactg gttcgccgac cagcgcgccg atggatgggc cacctgcccg gtgaccgaga
      661 acggcctcgt ccgcgtgctg tgcaatccac ggtacggcag tcccgaacac gcggtccctg
      721 ccgtcgtcga ccgtctccgg cagtttcgcg cgagcgggca tcatcagttc tgggccgacg
      781 aggtgtcgct gaccgacaac gcgctcttca agccgtcctt cgcgcgcggt catcgtcagg
      841 tcacggacgt ctatctgctg gggctcgcgc accgcaaggg cggtcgtctc gtcacgttcg
      901 accgctcgat tccgctgggc gcggtcgctg gtgccgtgcg agccacgctg gaggtcgtgg
      961 gctcagtcga cgacgattga ggcgagggcc ctacaatcca agcttgaccg gcgtcagctt
     1021 cggggacagc aggtggatcg cgccgagcgc gagcagatac gcggacgcgg caatgaggaa
     1081 gacagggacg tagctgccgg tcgtgtcgag gatgtagccg gtcagcttcg cgatcagcat
     1141 gccgccgacg gcgccggcca tgccgccgag gccgaccacc gatcccacgg cctggcgcgg
     1201 gaacgtgtcc gacaccagcg tgaagagatt tgcggaccag ccctgatgcg cggccgccgc
     1261 caggctcacg atcgcgacgg cgagccacag atcccgcaca ccggccgcca cggcgatggg
     1321 gagcacggcc agcgcgcacg ccagcatcgc cgtcttgcgc gcggtgttga cggaggcgcc
     1381 ccgcttcatc agcgccgacg acagccagcc cccgccgatg ctgcctgcgt cggcgacgag
     1441 atagatcatc acgagcggcg gaccgacgct ctgcagatcc aggccgtggt tccggttgag
     1501 gaagtccggg atccagaaaa ggtacagcca ccacaccgga tcggtcatga acttccccac
     1561 cgcgaacgcc cacgtctcgc ggtgcctgag cagggttctc cagggcacgc gcaccgtcgg
     1621 ctcgggcggg tcgctacgga tgtgcgcgag ctcggccgtc gagagcctcg cgtcgtgctc
     1681 gggcgcccga tacccgatcc accaccaggc aagccagaag aacccgatgg cgccggtggc
     1741 gacaaacgcc cagtaccatc cccacgtgat cgtgatccac ggcacgacga gcggcgtgac
     1801 gagcgccccg acgttggtgc cggagttgaa aattccggtg gcgaacgcgc gctcgcgctt
     1861 cgggaaccac tcggcgacga ccttgattgc ggccgggaag ttgcccgcct cgccgaggcc
     1921 cagcgcgaag cgggcggcga tgaagccggc caccgacgcc gtgtaggtca ggcccgcgat
     1981 cgagaggatc accgccgcga cgccgccgaa ggcttccgct tcggcatgcg ccatcgccgc
     2041 cacgctccac accacgatgg cgagcgcgaa tccggt
//
LOCUS       DBNGSH010000631         2393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3460540, whole genome shotgun sequence.
ACCESSION   DBNGSH010000631 DBNGSH010000000
VERSION     DBNGSH010000631.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3460540"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..322)
                     /locus_tag="AC16CY_14605"
     CDS             complement(155..322)
                     /locus_tag="AC16CY_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355076.1"
                     /translation="MIASIIVFGSIVFAAGFCLAWLVRPEVRAWLETPKHRFQASVRQ
                     YEDTAVRSVRR"
     gene            complement(319..1563)
                     /locus_tag="AC16CY_14610"
     CDS             complement(319..1563)
                     /locus_tag="AC16CY_14610"
                     /inference="COORDINATES: protein motif:HMM:NF014486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HIR3355077.1"
                     /translation="MTPRTGRAVRMSYVVASVAGVAFFVMSVALLAVWPGRVLDRQAR
                     AMSPERPLGLTPSQLRGRAVYSREGCAYCHTQQIRYVHADMVRFGAPTLAWETRLDYP
                     HLWGTRRIGPDLARAGATRTDDWHFAHLYAPRSVVSASVMPSYAWLFDGAPDRPRQSA
                     RDLVDYLQSLGRAREVAGPEGEARARGGCNCPDDAMAQMAFNAALNASAARPRRGAEA
                     PALAQNGDEARGQILYAANCASCHGVRGNGDGPGASSLRPRPADLSAHEYTLDRLSDA
                     LWNGVAGTAMQAWRDHPLEDLSAMARAVKNFQVPQEAIVPETFVALGQRVYAANCVQC
                     HGVNGRGDGSAARELSTAPTDFTRQRPTLGYSISVMRNGVEGTRMASWTGRISEAELV
                     AVANYVRTLFEPDEAPARGRTQ"
     gene            complement(1560..>2393)
                     /locus_tag="AC16CY_14615"
     CDS             complement(1560..>2393)
                     /locus_tag="AC16CY_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008041725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR3355078.1"
                     /translation="LWIHDAVGLFVTPMALAILYFVIPAASGKPIFSHFLSMLGFWLL
                     FFVYPLNGTHHYVFSVIPMEAQLTAIAASTILGVDVVIVVANLLLSLRGSGVFARDPG
                     LKFVAMSTIFYLVVSIQGSVQAQMSINQAVHFSDWVIGHSHLAMLGFATFAAAGGLVH
                     AWQRIPWARYNGRAVTWAYWLLLAGVVMMVTALTVAGIVEARLWRSGAPWLDSVVAAR
                     PFWLHRSFSAIPIAAGFIALLVGLTTGPRGGGLVAIATRDHVDSVEHVSPRLAAAGEA
                     S"
BASE COUNT          428 a          813 c          810 g          342 t
ORIGIN      
        1 gcaagggcgc acacgatgac ggccagggtc atgctcccgc gttggcgatc cgcgcgttga
       61 tcagaagtgt gcatcgcgtc acggtccggc tgccgccttc gcgcttcgcg cttcggcgcc
      121 caagaaagcc ggacactacg tctgtcaggt caggctaacg ccggacacta cgtacggcag
      181 tgtcctcata ctgccgcacg ctcgcctgga agcggtgctt cggcgtttcc agccacgccc
      241 ggacctccgg cctcacgagc cacgcaagac agaacccggc ggcgaagacg atagacccga
      301 acacgatgat gctggcgatc actgtgtgcg ccctctggca ggtgcctcgt ccggctcgaa
      361 gagcgtgcgc acgtaattgg ccacggcgac aagctcggcc tcgctgatgc gtcccgtcca
      421 cgacgccatg cgcgtcccct caacgccgtt gcgcatgacg ctgatgctgt aaccgagcgt
      481 tgggcgttga cgggtgaagt cggtcggcgc cgtcgagagc tcgcgggccg ccgagccgtc
      541 tcctcgtccg ttgacgccat gacactgcac gcagttggcg gcatagacgc gttgcccgag
      601 cgccacgaaa gtctcgggaa cgatcgcctc ctgaggcacc tggaagttct tcaccgcgcg
      661 cgccatggcc gagagatcct cgagcgggtg atcccgccag gcctgcatcg ccgttccggc
      721 cactccgttc cacagcgcgt cgctcaagcg atcgagtgtg tactcgtgcg cggaaaggtc
      781 ggccggccgg gggcgcagcg acgaggcgcc tgggccgtct ccgttcccac gcactccgtg
      841 gcagcttgcg cagttggccg cgtacaggat ctgtccccga gcctcatcgc cgttttgggc
      901 gagcgccggc gcctccgcgc cacgacgtgg ccgcgcagca ctcgcgttca gcgcagcgtt
      961 gaacgccatc tgggccatcg cgtcgtcggg gcagttgcag ccgccgcgag cccgggcttc
     1021 gccttccggg ccggcgacct cgcgcgcgcg cccgaggctc tggaggtagt ccaccaggtc
     1081 gcgcgccgac tgtcgcggac ggtccggcgc accgtcgaag agccaggcat atgaaggcat
     1141 gacggaggcc gagacgacag atcgcggcgc gtagagatgc gcgaaatgcc agtcgtcggt
     1201 ccgcgtcgct cccgcgcgcg cgagatccgg gccgatccgt ctcgtgcccc acagatgcgg
     1261 gtaatcgagc cgtgtttccc acgccagcgt cggcgcgccg aaacgcacca tgtcggcgtg
     1321 cacgtagcgg atctgctgcg tgtggcagta tgcgcacccc tcgcggctat agaccgcacg
     1381 gccgcgcagt tgactgggtg tgagcccgag cggtcgctcg ggtgacatag cgcgtgcctg
     1441 ccgatcgagc acgcggccgg gccacacggc caggagcgcg acggacatga cgaagaacgc
     1501 cacgccggcc acagacgcaa ccacgtacga catccggacg gcgcggccag tacggggcgt
     1561 cacgaggctt ccccggctgc cgccaatcgc ggcgacacgt gttcgacaga gtccacgtgg
     1621 tcccgcgttg cgatcgccac aagtccgccg ccccgtggtc ccgtcgtcag gccgaccagc
     1681 agcgcgatga agccggcggc gatcgggatg gcggagaagg atcggtggag ccaaaacggg
     1741 cgagctgcga cgaccgagtc gagccagggc gcgcccgacc gccacaaccg cgcttcgacg
     1801 atgccggcca cggtgagagc cgtgaccatc atgacgacgc ccgcgagcag cagccagtag
     1861 gcccacgtga ccgcgcggcc gttgtaccgc gcccacggaa tccgctgcca cgcgtgcacg
     1921 agtccgcctg ctgcggcaaa ggtcgcaaag ccgagcatgg ccaggtgaga gtggccgatg
     1981 acccagtcgg agaagtgcac cgcctggttg atcgacatct gcgcctgcac ggatccctgg
     2041 atgctgacga ccagatagaa gatcgttgac atcgccacga acttcagccc cggatcgcgc
     2101 gcgaagacgc ccgagccccg cagcgagagc agcaggttcg cgacgacgat gaccacgtcc
     2161 acgccgagga tcgtcgaagc cgcaatcgcc gtcagctgcg cttccatggg gatgacggaa
     2221 aagacgtagt ggtgtgtccc gttcagcgga tacacgaaga agagcagcca gaagccgagc
     2281 atcgacagga agtggctgaa gatcggcttg ccggatgcgg ccgggatcac gaagtagagg
     2341 atggccagcg ccatgggcgt gacgaagagg ccgacggcgt cgtgaatcca gag
//
LOCUS       DBNGSH010000632         3969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1762660, whole genome shotgun sequence.
ACCESSION   DBNGSH010000632 DBNGSH010000000
VERSION     DBNGSH010000632.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1762660"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="AC16CY_14620"
     CDS             complement(<1..275)
                     /locus_tag="AC16CY_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HIR3355079.1"
                     /translation="MNDCLFCRIVGGQIPARFVHEDHELVAIRDINPQAPLHVLIIPR
                     RHIGTLNDLGPHDDGLVGAMHRAAAAIAKEHGHAEDGYRTVFNCNRAA"
     gene            complement(272..1144)
                     /gene="prmC"
                     /locus_tag="AC16CY_14625"
     CDS             complement(272..1144)
                     /gene="prmC"
                     /locus_tag="AC16CY_14625"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3355080.1"
                     /translation="MTPDPITFLHEHLAAGRQSLVAAGIRPADAAIDVDLFARTILGW
                     DRARVLTARAEPAPPALEPRFSEWILRRARREPSAYIVGTREFWGLDFRVTPDVLIPR
                     PETEFIVEEALAILAGLRLASPRLADIGTGSGCLAVSLAREVPSATMTATDVSAAALE
                     VARENARRHHVDDRVTFVETSFLDGIDGPFDVIAANPPYVKDGDKTGLTRDVRYEPEV
                     ALFGGTSGLNGVEAALGAGAGKLRSGGWLVMEFGFGQEDDVRRLVAAHPALTVHHVRE
                     DLQGIPRTAVIQKR"
     gene            complement(1164..2258)
                     /gene="prfA"
                     /locus_tag="AC16CY_14630"
     CDS             complement(1164..2258)
                     /gene="prfA"
                     /locus_tag="AC16CY_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR3355081.1"
                     /translation="MFDKLTLVESRYDELMTKLGSVAVQSDPSEFKKASKALAELEPL
                     VQKFREFKGVELDIAGAEELFRSADADMRELADEELRALGVRREALLQELKVLLVPKD
                     PNDEKNVILEIRAGAGGDEAALFAGDLFRMYSRYAERQRWKLEVLSSSETGAGGLKEA
                     IASIEGKGVYSRLKYESGVHRVQRVPATEASGRIHTSTATVAVLPEAQEVDIQINEKD
                     LRIDTFCSSGPGGQSVNTTYSAVRITHLPTGLVVSQQDEKSQIKNRSKAMKVLRARLY
                     EMEMQKQQDAIAKERRGQVGTGERSEKIRTYNFPDSRITDHRVNFKTSRVHDVLDGDL
                     GQLIDEVISFYNTQKLKEVTTVEETGPTKH"
     gene            complement(2342..2587)
                     /locus_tag="AC16CY_14635"
     CDS             complement(2342..2587)
                     /locus_tag="AC16CY_14635"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3355082.1"
                     /translation="MRVTVRLFARLRDLAGSGELVRDIDGPATVHTVWKGLVAEMPAL
                     GDYERTMSVAVNADYSRMSASVSDGDEVAFLPPVSGG"
     gene            complement(2578..3060)
                     /locus_tag="AC16CY_14640"
     CDS             complement(2578..3060)
                     /locus_tag="AC16CY_14640"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR3355083.1"
                     /translation="MGEPFVAVTSVPLDLQALTREVAGSCVGDGAVASFIGLVRDHNQ
                     GRRVSYLDYEAYEPLAVRTLGRIVDEVRSNWPDTRVGVHHRTGRIEIGDASVIIVAAS
                     PHRANAFAACRYTIERVKQIAPIWKHEHFEGGDVWLEGATADPDDESARQAAYRIACA
                     "
     gene            complement(3074..3862)
                     /gene="thiD"
                     /locus_tag="AC16CY_14645"
     CDS             complement(3074..3862)
                     /gene="thiD"
                     /locus_tag="AC16CY_14645"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR3355084.1"
                     /translation="MSPRRVALTIAGSDSGAGAGIQADLKTFAAHGVYGVCAVTAVTA
                     QNSLGVTMVEALPADLVAGQIEAVVSDFGAHAAKTGMLANAAIVEAVAAAIRDLEIPF
                     LVVDPVMLAKSGDALLDEEALAAMKAELLGRAFLVTPNIPEAEALAGLAIRSDEDRRE
                     AARRIAALGPAAVVIKGGHFPSENITDLLYTRSEFVEFPQPRVPGRNTHGTGCTFSAA
                     VTAHLAKGRSLREAIPLVQQYVAGAIRHAPDLGRGAGPMEHFWH"
BASE COUNT          623 a         1371 c         1293 g          682 t
ORIGIN      
        1 gcggcgcgat tgcagttgaa caccgtgcgg tagccgtcct cggcgtgccc gtgctctttg
       61 gcgattgccg ccgccgcccg atgcatcgcc cccacgagcc cgtcatcgtg aggtccaagg
      121 tcgttcaacg tgccgatgtg gcgacgcggg atgatcagca cgtgcagcgg cgcctgcggg
      181 ttgatgtccc tgatggcgac cagttcgtgg tcctcgtgaa cgaaccgggc gggaatctgt
      241 ccgccgacaa tcctgcagaa gaggcaatcg ttcatcgctt ctgaatcact gcagtgcggg
      301 gaatgccctg caggtcctcg cgcacgtggt gcactgtgag cgccgggtgt gccgccacga
      361 gccgccgcac gtcgtcctcc tgaccgaaac cgaactccat gacgagccag ccgccgctgc
      421 gcagcttccc ggcgccggcg ccgagcgcgg cctcgacgcc gttcaggccg ctcgtgcctc
      481 cgaacagcgc aacctcgggt tcgtaccgga cgtcgcgcgt cagtcccgtc ttgtcaccgt
      541 ccttcacgta cggcgggttt gccgcgatga catcgaaggg accgtcgata ccatccagaa
      601 acgacgtctc gacgaatgtc acgcggtcgt ccacatgatg ccgcctcgcg ttctcgcgcg
      661 cgacctcgag ggccgctgct gacacgtcgg ttgccgtcat cgtcgccgag ggcacttcgc
      721 gggccaggct gacggcgagg cacccgctcc ccgtaccgat gtccgcgagc ctcggcgacg
      781 ccagccgcag accagcgagg atggcgagcg cttcctcgac gatgaactcg gtttcagggc
      841 gcgggatgag cacgtccggc gtgacgcgga agtccaaccc ccagaattcc cgcgtgccga
      901 cgatgtaggc ggacggttcg cgcctggccc tgcggaggat ccactccgag aagcgcggct
      961 cgagcgcggg tggcgcgggc tccgcgcggg cggtcaacac gcgggcccgg tcccacccga
     1021 ggatcgtccg cgcgaacagg tccacgtcga tcgccgcgtc ggccggcctg atgccggcgg
     1081 cgacgaggct ctgccggcct gcggccaggt gctcatgcag aaatgtaatg gggtcgggag
     1141 tcatttttcg aggacgccag cagtcaatgc ttcgtcggcc cggtttcctc gaccgtcgtg
     1201 acttccttga gcttctgggt gttgtagaag gagatgacct cgtcgatcag ctgtccgagg
     1261 tcgccgtcga gcacgtcgtg cactcgactg gtcttgaagt tgacgcggtg atcggtgatg
     1321 cggctgtcgg ggaagttgta ggtgcgaatc ttctcagagc gctcgccggt tcccacctgg
     1381 ccgcgccgct ccttcgcgat cgcatcctgc tgcttctgca tctccatctc gtagagacgc
     1441 gcacgcagca ccttcatggc cttcgaccgg ttcttgatct gcgacttctc gtcctgctgc
     1501 gagacgacga ggccggtcgg cagatgggtg atccggacgg cggaatacgt cgtgttgacg
     1561 ctctggccac cgggcccgct cgagcagaac gtgtcgatgc gcaggtcctt ctcgttgatc
     1621 tgaatgtcta cttcctgggc ctcggggagg acggcaaccg tcgccgtgga cgtgtgaatg
     1681 cggccgctgg cctccgtcgc cggcacccgc tgcacgcggt gcacgccgct ctcgtacttc
     1741 agccggctgt agacgccttt gccttcgatc gaggcgatgg cttccttcag cccgccggcg
     1801 cccgtctcgc tgctcgacag gacttcgagc ttccatcgct ggcgctcggc gtaccgggag
     1861 tacatccgga acagatcccc cgcgaacaac gcggcttcgt cgccgcccgc gcccgcgcgg
     1921 atctcgagga tgacgttctt ctcgtcgttg ggatccttcg gcacgagaag aaccttcagc
     1981 tcctggagca gggcttcgcg cctgacgccc agcgcgcgca gctcctcgtc ggccagctcg
     2041 cgcatgtcgg catcggcgct ccggaacagc tcctcggccc ccgcgatgtc gagctcgacg
     2101 cccttgaatt cgcggaactt ctggacgagc ggctccagct ccgccagcgc cttggacgcc
     2161 ttcttgaact cggaggggtc cgactgcacg gcgaccgagc cgagcttggt catcagctcg
     2221 tcgtagcgcg actccaccaa agtgagcttg tcgaacatgg gcctttcacg gtccgcctaa
     2281 aggcggacac tacgtactcg cggtccggct gaagccggac gctacgtact aacaacatca
     2341 attatccccc ggagaccggg ggaaggaacg ccacctcgtc gccgtcgctc accgacgccg
     2401 acatgcgtga ataatcggca ttgaccgcaa ccgacatcgt ccgctcgtag tcgccgagcg
     2461 ccggcatctc cgcgacgagc cctttccaga ccgtgtgaac cgtcgccgga ccgtcgatgt
     2521 cgcgcaccag ctcgcccgag ccggccagat cgcgcagccg cgcaaagagc ctcaccgtca
     2581 cgcgcatgcg atacggtacg ccgcctgccg ggccgactcg tcatcgggat cggccgtggc
     2641 gccctccagc cagacgtctc cgccttcgaa gtgctcgtgc ttccagatcg gagcgatctg
     2701 cttgacgcgc tcgatcgtgt accggcacgc cgcgaaggcg ttcgcgcggt gaggcgaggc
     2761 cgccacgatg atgacgctcg cgtccccgat ttcgatgcgg cccgtcctgt ggtgcacgcc
     2821 gactcgcgtg tcgggccagt tgctgcggac ctcgtccaca atccggccga gcgtcctcac
     2881 ggccagcggc tcgtaggctt cgtagtcgag gtacgagact cgccgtccct gattgtggtc
     2941 gcgtaccagg ccgatgaagg acgcgacggc gccgtcgccc acacaggagc cggcgacctc
     3001 cctggtcagg gcctggaggt cgagcgggac cgaagttacg gccacgaagg gctcacccat
     3061 gggatcagta tagctagtgc cagaagtgtt ccatgggtcc cgcgccgcgg cccaggtcgg
     3121 gtgcgtgccg aatcgcgccg gcgacgtact gctggacgag cgggatcgcc tcccgcagcg
     3181 agcggccctt tgcgagatgc gccgtgaccg cggccgagaa cgtacatccg gttccgtgcg
     3241 tgttgcggcc cggcacgcgc ggctggggaa actcgacgaa ctcactcctg gtgtagagca
     3301 ggtctgtgat gttctcggag gggaagtgac cgcccttgat cacgacggcc gccgggccca
     3361 gcgcggcgat gcgccgggcg gcttcccgcc gatcctcgtc cgatcgaatg gccaggccgg
     3421 ccagcgcttc tgcctccggg atgttcggcg tgacgagaaa cgcccgcccg agcagctcgg
     3481 ccttcatggc tgcgagcgct tcctcgtcca ggagtgcatc gcccgacttt gccaacatga
     3541 ccggatcgac gacgaggaag ggaatctcga gatcgcgaat cgcagccgcg acggcctcga
     3601 cgatcgctgc gttggccagc atcccggtct tggccgcatg cgcgccgaag tcggacacga
     3661 ccgcttcgat ctggcccgcg acgagatcgg ccgggagcgc ttcgaccatc gtgacgccca
     3721 gcgagttctg cgccgtgacg gccgtgacgg cgcacacgcc gtagacgccg tgcgcggcga
     3781 acgtcttgag gtcggcctgg atgccggcgc ccgcgcccga atcgctcccc gcgatcgtca
     3841 gcgccacgcg gcggggactc acgctggaac cctcaggtcg tcaacgcgct cgtccaacca
     3901 ggcgccagag cgcgggaacc agacccgccg ccacggcaag cttgatcaga tcgggtatca
     3961 cgaacggaa
//
LOCUS       DBNGSH010000633         1909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2441566, whole genome shotgun sequence.
ACCESSION   DBNGSH010000633 DBNGSH010000000
VERSION     DBNGSH010000633.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2441566"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="AC16CY_14650"
     CDS             <1..1044
                     /locus_tag="AC16CY_14650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3355085.1"
                     /translation="RAVGWSDKEAGRRMTADTIFRIASQTKAITSVAILSLAEEGRLT
                     LADPVSRTIPPFAKTTVAAGGSTAPAKRAMTIRDLLTHTAGISYGTEPAIASQYEAKG
                     LGPAAGFGWYTADKDEPVCSTMERLATLPFVAQPGEQWVYGYNTDILGCVVERASGMP
                     LDAFVRTRITEPLRMTDTQFFLPAAQRTRLAAVYASGADGLIARAPDGPRGQGNYVDG
                     PRRSFAGGAGLLSTARDYGRFLEMIRRGGELDGVRVLSSRSVKLMTTNQVGTLHSAAG
                     LGFGLGFQTTDRYGANGMDSEGAFGWGGAYGTVYRVDPEARLVMLLMIQLLPNSTDIR
                     TVFPTLVYQALEQ"
     gene            complement(1071..1487)
                     /locus_tag="AC16CY_14655"
     CDS             complement(1071..1487)
                     /locus_tag="AC16CY_14655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3355086.1"
                     /translation="MKRIAPLLALLVLASCDEGSTTGPTTTAPYSQTDLVVGTGATAA
                     NGNLVTVAYTGWLHDSSRPDSKGDQFDSSASFPFRLGTGAVIQGWDMGVVGMRVGGQR
                     RLVIPPELAYGNTSPGAGIPPNATLVFDITLNNVQP"
     gene            1714..>1909
                     /locus_tag="AC16CY_14660"
     CDS             1714..>1909
                     /locus_tag="AC16CY_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3355087.1"
                     /translation="MSKEVLQGTLDLMVLRTLETMGPQHGFGLAKRILQISEGILDLN
                     QGTLYPALVRLEQRRWIRSRW"
BASE COUNT          334 a          628 c          642 g          305 t
ORIGIN      
        1 cgtgccgtcg ggtggagcga caaggaagcc ggaaggcgga tgacggccga caccatcttc
       61 cgcatcgcct cgcagaccaa ggcaatcacc agcgtggcaa tcctgtcgct cgccgaggaa
      121 ggcaggctca cgctcgccga cccggtcagc cgcacgattc cgccgttcgc gaagacgaca
      181 gtcgctgccg gaggctcgac ggcgccggcg aagcgtgcga tgaccattcg cgacctcctc
      241 acgcacacgg ccggcatctc gtacggcacc gaacccgcca ttgcgtccca gtacgaggcc
      301 aagggcctcg gccccgccgc cggtttcggg tggtacaccg ccgacaagga cgagcccgtc
      361 tgctcgacga tggagcgcct ggcgacgctg ccgttcgtgg cccagccagg ggagcagtgg
      421 gtgtacggct acaacaccga catcctgggc tgtgtcgtcg agcgggcgtc gggaatgccg
      481 ctcgacgcgt tcgtcaggac gcgcatcacc gaaccgcttc ggatgaccga cacgcagttc
      541 ttcctgcctg ccgcgcagcg aactcggctg gcggccgtct acgccagcgg cgctgacggg
      601 ctgatcgccc gggcgcccga cggcccgaga gggcagggca actacgtcga tggtccacga
      661 cgcagcttcg ccgggggtgc gggcctgctc tcgacggctc gcgactacgg acggtttctc
      721 gaaatgatcc gccgcggcgg cgagctcgat ggcgtccgcg tcctgtcatc gcggagcgtc
      781 aagctcatga ccacgaacca ggtcggcacg ctgcactccg cggccggact cgggttcggg
      841 ctcggcttcc agacgacgga tcggtacggc gccaacggca tggattcgga gggagccttc
      901 ggatggggcg gcgcctacgg cactgtctat cgcgtcgatc cggaggcacg gctcgtcatg
      961 ctgctgatga ttcagctgct gccgaactcg acggatatca ggaccgtgtt cccgacgctc
     1021 gtgtatcagg ccctcgaaca gtagccgtgc gcccggcggc gcgctgacgt ctacggctgg
     1081 acgttgttca acgtgatgtc gaagacgagc gtcgcgttgg gcgggatgcc tgccccgggg
     1141 ctcgtgttgc cgtaggcgag ctcgggcgga atcaccagcc ggcgctgccc gccgacgcgc
     1201 atgccgacga cgcccatgtc ccaaccctga atcacggcgc cggtgccgag gcggaacggg
     1261 aagctggcac tcgagtcgaa ctggtcgccc ttcgaatcgg ggcggctcga gtcatgtagc
     1321 caacctgtgt acgcgacagt gaccagattg ccgttggccg ccgtcgcgcc ggtgccgacg
     1381 acgagatcgg tctgcgaata gggtgcggtc gtggtcgggc ccgtagtgga gccttcgtcg
     1441 cacgacgcga ggaccagcaa tgcgaggagt ggggcgatac gcttcatcat cccgagtgta
     1501 gacgatcgcc gcggagaaat ggcctgcacc gcgcgaagcg gcgctctcac gaatcagggc
     1561 gtgactcctg ttgacattct aaatgagacg atccatggtc gtttagtgga ctgtttcaac
     1621 tggaagagta ccgcgaacaa cgagcttcga ccgcgattct ccggccgcga caccggagcg
     1681 gccggtagca attctcgaaa gacataccac tagatgtcaa aagaagtcct ccagggcacg
     1741 ctcgacctga tggtcctccg cacgctcgaa accatggggc cgcagcacgg cttcggcctg
     1801 gcgaagcgca tcctccagat ctccgagggc atcctcgatc tcaaccaggg gacgctgtac
     1861 ccggcgctcg tgcggctcga gcagcgccgc tggatccggt cgcgatggg
//
LOCUS       DBNGSH010000634         2190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2950802, whole genome shotgun sequence.
ACCESSION   DBNGSH010000634 DBNGSH010000000
VERSION     DBNGSH010000634.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2950802"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(424..>2190)
                     /locus_tag="AC16CY_14665"
     CDS             complement(424..>2190)
                     /locus_tag="AC16CY_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3355088.1"
                     /translation="TPLMVGGRLFSTAGTRRAVVALDAATGELLWVHGEDEGARGAAA
                     PRQLSGRGLAYWSDGKEERILYVTPGYHLVALDAKTGSRAASFGRNGAVDLKQDFDQP
                     FDLTTGPVGLHATPTVAGNIVLVGAAFETGANPRSRSNIKGAVRAFDVRTGKRLWAFK
                     TIPEPGEFGSETWEDDSWAYTGNTGVWAQISVDEELGIAYLPVEMPTHDYYGGARPGN
                     GLFGESLVAVDLKTGQRKWHFQLVHHGIWDMDIPCAPILADITINGRTVKAVAQPSKQ
                     AFLYVFDRVTGQPIWPIEERQVPQGDVPGERYSKTQPFPTRPAAYERQGLAIDDLIDF
                     TPELRAEAVKAIAPYKIGPVFTPPVVSRAEGPIATLQMASQAAATNWPGGSYDPETHV
                     LYVNSQSAVATLGLVPPPPGAAPDVRYHQGTVLSGPRLAGGSGAAGGGGTAVTAPTVQ
                     GLPLIKPPYGRLSAISLDTGTILWQVPHGETPDTIKNHPALKGLTIPRTGRPGHVGSL
                     VTRTLVISGEPGFGTTASGQRGAMLRAYDKKTGADAGAVYMPAPQTGSPMTYMLNGRQ
                     YLVVAISGGSYSGELLAFRLPV"
BASE COUNT          353 a          729 c          713 g          395 t
ORIGIN      
        1 gacactgaac gccgtctgag cctgagcgcg agtggaaacc aacgtcgcgc cgaggacgaa
       61 cgagaggatg gcaagtttgc gcatctgatt catctcccgg cgtgtgcttc agcaagcgcc
      121 ctgccgggag ctgagccagc ctaagtgtcg tcaggacggc cccttacggg cgtggccgtt
      181 tatggacgag gcgttcttgt cccctcgaaa tgacagtgag gatgtagatc tgtaactcgg
      241 ccgtgccagt gcatttgtga ttagtggatc tttgattggt gattccttgg tgattagaga
      301 ttcttgactg gtgatttatt ggcgattggc gtcgtgcgat tcagcccggc ccgtcaatca
      361 acaatcgaca aatcggcaat caacaacgat tcaacaaatc aaagatcggt caatcacaaa
      421 tgatcacacc gggaggcgaa aggcgagcag ttctcccgag tagctgccgc cgctgatcgc
      481 gacgacgagg tactgcctgc cgttcagcat gtaggtcatc ggcgatccag tctgcggcgc
      541 cggcatgtag acggcgcccg cgtcggcgcc ggtcttcttg tcgtacgcgc gcagcatcgc
      601 gcctcgctgc cctgacgccg tcgtgccgaa gccgggctcg ccggaaatca cgagcgtcct
      661 ggtgacgagc gagccgacat ggccgggacg tcccgtgcgg ggaatggtca agcccttgag
      721 ggcggggtga ttcttgatcg tgtccggcgt ctctccgtgc gggacctgcc agaggatggt
      781 gcccgtgtcg aggctgatcg cactgaggcg tccgtacggc ggcttgatga gcggcagacc
      841 ctgaaccgtc ggcgccgtga ccgcggtgcc cccaccgccc gccgcgcccg atccacccgc
      901 gagtcgagga cccgacagca cggtgccctg gtgatagcgc acgtccggcg cagcgcccgg
      961 cggaggaggc acgagaccga gcgtggcgac cgcgctctgc gagttcacgt agagcacgtg
     1021 cgtctcggga tcgtaggagc ctcccggcca gttcgtcgcg gcggcctgac tggccatctg
     1081 cagcgtcgcg atcgggcctt ccgccctgct gacgacgggc ggcgtgaaca ccggcccgat
     1141 cttgtacggc gcgatcgcct tcaccgcttc cgcccggagt tccggcgtga agtcgatcag
     1201 atcgtcgatt gccagcccct ggcgctcgta cgccgcaggc ctggtcggga agggctgggt
     1261 cttcgagtac ctctctcccg gcacgtcacc ctgcggcacc tggcgctcct cgatcggcca
     1321 gatcggctga ccagtcacgc ggtcgaagac gtaaaggaag gcctgcttgc tcggctgcgc
     1381 caccgccttg accgtccggc cgttgatcgt gatgtcggcg aggatcggcg cgcacggaat
     1441 gtccatgtcc cagatgccat ggtgcacgag ctggaagtgc cacttccgct gacccgtctt
     1501 cagatcgacg gcgaccaggc tctcgccgaa cagtccgttc cccggacgcg cgccgccgta
     1561 gtagtcgtgc gtcggcattt caacgggcag gtacgcgatc cccagttcct cgtccaccga
     1621 gatctgcgcc cacacgccgg tgttgccggt gtaggcccac gagtcgtctt cccacgtctc
     1681 gcttccgaac tcgcccggct cgggaatggt cttgaacgcc cacaggcgct tgcccgtccg
     1741 gacgtcgaag gcacggacgg cgcccttgat gttgcttcgg ctcctcgggt tggcgcctgt
     1801 ctcgaaggcg gcgccgacca gcacgatgtt gccggcaacg gtgggcgtgg cgtgcagtcc
     1861 gacgggcccc gtcgtcagat cgaatggctg atcgaaatcc tgcttgagat cgacagcgcc
     1921 gttgcgtccg aacgacgcgg cccggctccc tgtcttcgca tcgagcgcga ccaggtgata
     1981 gcccggcgtg acgtagagga tccgctcctc cttgccgtcg ctccagtacg ccagccccct
     2041 gccggagagc tgccgcggcg ccgccgcgcc gcgcgcccct tcgtcttcac cgtgcaccca
     2101 cagcagctcg ccggtcgcgg cgtcgagcgc aacgaccgcg cgccgcgtgc cggcagtcga
     2161 gaacaggcgt ccgccgacca tgagcggcgt
//
LOCUS       DBNGSH010000635         2219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3630312, whole genome shotgun sequence.
ACCESSION   DBNGSH010000635 DBNGSH010000000
VERSION     DBNGSH010000635.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3630312"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="AC16CY_14670"
     CDS             complement(<1..169)
                     /locus_tag="AC16CY_14670"
                     /inference="COORDINATES: protein motif:HMM:NF020922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HIR3355089.1"
                     /translation="MAGPFAGFPRESTQFFVDLARHNNREWFQAHKEIYERACREPMQ
                     ALAAEFEPLGPT"
     gene            complement(292..975)
                     /locus_tag="AC16CY_14675"
     CDS             complement(292..975)
                     /locus_tag="AC16CY_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355090.1"
                     /translation="MADRPRVIVVLPNAAESAEVADWLVAEGLEPVLRSAPRAAEEMR
                     DRPFALLISDAASAIRGGLQAQGRARNPHTVTILVGESEAGAQAMSSQAMFLTRPIDR
                     ATLTCFVSMALMDGRPARRSVRKSINRFDALVNGVPSHLLDVSAEGLRLEVGKDRRAV
                     LGPYFKVKVPLVGVTVTVQRMWTRASSNGVPTLWYGGALTQNQANVDQAWRTFVDTVP
                     VAAGLQTNP"
     gene            1096..>2219
                     /locus_tag="AC16CY_14680"
     CDS             1096..>2219
                     /locus_tag="AC16CY_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR3355091.1"
                     /translation="MFTVQPPSGPYQLNLQRAGQPCDADFAGQPTGGPLCVSLICSTR
                     TAILTAVLGLWTLSLAAQAPPAPPPLRPPTRADLLRGEYGRYRANNDLLFYHLDVRVD
                     PGKKSIAGKNTIRFRMLADDTRIQLDLARNLAVDRILLDGTPLTFERELDTVWVDFPA
                     TLRAGREYAIDFYYSGQPREQGRFGGMSFGKDPAGRHWITTSCEDNGARVWWPNKDQW
                     RDEVERMQISVAVPNDLVDVSNGRFLGKTDLGDGYTRWDWLVQYPINNYNVALNIGRY
                     EHFADRLGDLPLDFYVLPENLDKARRQFAQAAPMLEAFQKYFGEYPFAKDGFKLIEVP
                     YAGMEHQSAVAYGNRFANGYLERDWTGVGTSLKFDFIIVH"
BASE COUNT          399 a          731 c          726 g          363 t
ORIGIN      
        1 gtgtaggtcc gagcggctcg aactcggcgg cgagcgcctg catcggttca cggcacgccc
       61 gctcgtagat ctccttgtgg gcctggaacc attcccgatt gttgtgccgc gccagatcga
      121 cgaagaactg ggtggactcg cggggaaacc ccgcaaaggg accggccatg ctgacctccg
      181 gccgatctta gcctcaacgc gtcaagaacg aggtctgtcc ccccagggac ggaccaggct
      241 cgaagaacca cggcccaagg actggccgcc ttttgatcag ggttagtctg gctagggatt
      301 ggtctggagc ccggctgcga caggaacggt gtccacgaac gtgcgccagg cctggtcgac
      361 gtttgcctgg ttctgcgtca gcgctccgcc gtaccagagg gtcgggactc cattcgacga
      421 agcccgggtc cacatccgct gcacggtgac cgtgacgccg accagcggaa cctttacctt
      481 gaaatacgga cccaggactg ccctgcgatc cttgcccact tcgaggcgaa ggccctcggc
      541 gctgacatcg agcagatggg aagggactcc gttcaccaga gcgtcgaagc ggttgatcga
      601 cttcctcacg gagcggcgcg cgggccttcc gtccatgagc gccatcgaga cgaaacaggt
      661 gagcgtcgcc cggtcgatcg gccgtgtcag gaacatggcc tggctgctca tggcttgcgc
      721 gcccgcctcg ctttcgccga cgagaatcgt cacggtgtgc gggttccggg cgcgcccctg
      781 tgcctgaagt ccaccgcgga tcgcggaagc ggcgtctgaa atcaggagag cgaacggccg
      841 gtcgcgcatc tcttccgcgg cgcgtggggc ggaccggagc acgggctcca ggccctcggc
      901 aacgagccag tcggcgacct cagcgctctc ggcggcattg ggcagtacga cgatcacgcg
      961 ggggcgatcg gccacggggc cgtatttgca ccgagtgtgc cagacagatt tcccagcgac
     1021 gcgagcagca caacggcgct gcgagtacgg atgtgcaact gccgggagag atctgtaacg
     1081 cgcaagtgag gcatgatgtt caccgtgcag cctcctagtg gaccgtatca actgaacctg
     1141 cagcgggcgg gacagccctg cgacgccgat ttcgcgggcc aaccaacggg cggcccgctt
     1201 tgcgtttcgc tgatttgctc cactagaacg gctatcctga cggcggtcct cgggctctgg
     1261 acgctgagcc tcgcggccca ggctccgccg gctcctccgc ccctgcgccc gccgactcgc
     1321 gccgacctgc tgcgcgggga gtacgggcga taccgcgcga acaacgacct gctcttctac
     1381 catctcgacg tccgcgtcga ccccgggaag aagtcgatcg ccggaaagaa cacgatccga
     1441 ttcaggatgc tcgccgacga cacgcggatt cagctcgatc tcgcgcgcaa cctcgccgtt
     1501 gaccggatcc tgctggacgg aacgcccctg acattcgagc gggagctcga cacggtctgg
     1561 gtggactttc cggcgacgct gcgagcgggg cgcgagtacg ccatcgactt ctactactcg
     1621 ggccagcccc gcgagcaggg ccggttcggg ggcatgtcgt ttggcaagga tccggcaggc
     1681 cggcactgga ttacgacgtc atgcgaggac aacggggccc gggtgtggtg gccgaacaag
     1741 gaccagtggc gggacgaggt cgagcggatg cagatcagcg tggccgttcc caacgacctg
     1801 gtggacgtct cgaacggccg ctttctgggc aagaccgacc tcggcgacgg ctatacccgc
     1861 tgggactggc tggtgcagta tccaatcaac aactacaacg tcgcgctgaa catcgggcgc
     1921 tacgagcact tcgccgatcg cctcggcgac ctgccgctcg acttctacgt gctgcccgag
     1981 aacctcgaca aggccaggcg ccagttcgcg caggcggccc cgatgctcga ggccttccag
     2041 aagtacttcg gcgagtaccc gttcgcgaaa gacggcttca agctgatcga ggtgccctac
     2101 gcgggcatgg agcaccagag cgccgtcgcg tacggcaatc gcttcgccaa cggatacctc
     2161 gagcgcgact ggaccggcgt cggcaccagc ctcaagttcg acttcatcat cgtgcacga
//
LOCUS       DBNGSH010000636         2675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3291535, whole genome shotgun sequence.
ACCESSION   DBNGSH010000636 DBNGSH010000000
VERSION     DBNGSH010000636.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3291535"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="AC16CY_14685"
     CDS             <1..207
                     /locus_tag="AC16CY_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355092.1"
                     /translation="KMGARANGVLRESFRKDGRMHDQVLWSLLESDWRRESNGLWQPS
                     TEIRLTDADRADSGSPISLPTLTH"
     gene            complement(324..551)
                     /locus_tag="AC16CY_14690"
     CDS             complement(324..551)
                     /locus_tag="AC16CY_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355093.1"
                     /translation="MAKQMTGSTRQETGAPTTKVMSGTVGAALASIVIWALQAASVPV
                     PDGTQIAITTVVTFMFGYYTPPAHRDVVRTA"
     gene            complement(833..1774)
                     /locus_tag="AC16CY_14695"
     CDS             complement(833..1774)
                     /locus_tag="AC16CY_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_289266904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR3355094.1"
                     /translation="MHTSDIAVGIDVGKHAVVIAVTPSGEQWTSATTPDQLEGTMARI
                     AHLRPTVVVLEATGGYEAPVAAAGAAAQLPLVIVNPRQVRAFAHALGRTAKTDAIDAH
                     VLALFGARVQPALRPLPDADTQALAALVTRRRQLLEMLTAERQRLEQARTRPVQRDLR
                     THIRWLERRVTDVDDDLDTTIRRSPLWLVQEDLLRSVPGIGPTVARTLLAELPELGTL
                     DRRAIAALVGVAPLNRDSGQHRGHRAIAGGRASVRAALYMAALVAARHNRPLRHFYQR
                     LRTAGKPAKVALVAVMRKLLTILNAMVKHQTAWQPPA"
     gene            complement(2227..>2675)
                     /locus_tag="AC16CY_14700"
     CDS             complement(2227..>2675)
                     /locus_tag="AC16CY_14700"
                     /inference="COORDINATES: protein motif:HMM:NF015148.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HIR3355095.1"
                     /translation="RDTYGIVGDNAQLLSPTSQRWVGFAKLVTQELSGPNASSPEAIA
                     RLSWITWSATIGALIGIALAWLEQKESWRPFIPSPAGMGIAMLIPVNAVTMIFTGALA
                     DRVWAKIDASTHERYSIPMASGLIAGEALVAIALPLLVTLGLMQLP"
BASE COUNT          424 a          911 c          893 g          447 t
ORIGIN      
        1 aagatgggcg cgcgggcaaa cggcgtcctc cgcgagtcgt tccggaagga cggccggatg
       61 cacgatcagg tgctgtggtc gctgctcgaa tcggactggc gccgcgaatc gaacgggctc
      121 tggcagccgt cgaccgagat ccgcctgact gacgccgacc gcgccgactc cggcagcccg
      181 atctctcttc ccactctgac acactgagca gatgcccctg gcccgccgac gctgcatagc
      241 ggcgaaggcg ggcccccctc gccgcaccct cgcaccttcg caccctcgca gcgtctaacc
      301 cgctcgaagc cgcccactga tcttcacgcc gtcctgacca cgtcccggtg cgctggcggc
      361 gtgtagtaac cgaacatgaa ggtcaccacg gtggtgatcg cgatctgagt gccgtcgggc
      421 accgggacgc tggccgcctg aagcgcccag atcacgatcg acgcgagcgc cgcgccaacc
      481 gttccgctca tcaccttggt cgttggggca ccggtttcct gtctcgtcga cccggtcatc
      541 tgcttcgcca tactgacgtc ctttcgcccg ctcggggcct ggggccggct gcgctcactc
      601 atgccggcct cacttccatc aacgggcgct gcagaggatc cgaacgggac cttgaggacg
      661 ctcttgggtc gagccacttg cctccgtgaa tcccggttcc ggtggccgga ccgcgctgcg
      721 agggccgccc ccggccgcgt gacgccagct ctgtgagctg gcggatcccc aaagccttct
      781 cgcgcggtca ggcgattcat gtcggcctga cctccacagc aactgtcttt cgttacgcgg
      841 gcggctgcca cgcggtttga tgtttgacca tcgcgttcag gatggtgagg agtttacgca
      901 tgacggcgac gagcgccacc ttcgccggtt tacctgcggt gcggagccgc tggtagaagt
      961 gccgcaaggg acggttgtgg cgcgcggcga ctaatgcggc catgtagagc gccgcgcgca
     1021 cggaggcgcg gccgccggca atcgcgcggt ggccgcggtg ctgtccacta tcgcggttga
     1081 ggggtgcgac tccgacaagc gccgcgatgg cgcgccgatc gagggtgccg agttcgggga
     1141 gttcggccag tagggtgcgg gcgacggtgg ggccgatgcc tggcacactg cgcagcaggt
     1201 cctcttggac cagccacagg gggctgcgcc gaatggtggt gtcgaggtcg tcatccacgt
     1261 ccgtgacgcg ccgctccagc cagcgaatat gcgtgcgcag gtcgcgttgc accggtcgcg
     1321 tgcgcgcctg ctcgagccgc tggcgctccg cagtcaacat ctccaggagt tgccgccgcc
     1381 gcgtgaccaa ggccgctaac gcctgtgtgt cggcatcagg caggggccgc agcgccggct
     1441 gcacgcgcgc gccaaacagg gcgagcacat gcgcatcaat ggcgtcggtc ttggccgtgc
     1501 ggccgagcgc gtgcgcgaac gctcggacct gccgcggatt gacgatcacc agcggcagct
     1561 gcgcggccgc ccccgccgca gcgacgggcg cctcatagcc tcccgtcgct tcgagcacga
     1621 cgaccgtcgg gcgcagatgg gcgatccggg ccatcgtgcc ttcgagctgg tccggggtgg
     1681 tcgcactggt ccactgctct ccgctcgggg tgacggcgat gacgaccgcg tgtttgccca
     1741 catcaatacc aacggcgatg tccgacgtgt gcatgacggt cctccgagtc accgcagctc
     1801 ccgctcttat gggcgtccgg gctcgaggcc cgctcaactg ttcgggatga acgatgacgt
     1861 tagcgagagg gcgcccatgc tgtggaacgg tctgcacacc cagggattta cgggctaccc
     1921 tctcaccccg ctttgtagca gaaggcgaag acattaggga ttcactgcgc aaatggctcg
     1981 acccaagagc tcgcaccgct tactcgggag ctcagtcgtg cgtggaatgg cggcaagccc
     2041 tacgagtcga cgcgatcagc gaagtgagta aaagcgcgct ggaggctcgc cgcgtggcca
     2101 cggcgttgcc gctagccggg aagataaacg tgtgccatcg ggagtgccgg aagcgcgctt
     2161 tttccgcgtc gatcgagcgg gctcgcccga cattccacac gactgagctc ccgagcgatc
     2221 aggcctctag ggcagttgca tcaggccgag cgtcaccagc agcggcaggg caatggcgac
     2281 gagggcttct cccgcaatca gccccgacgc catcggaatc gagtagcgct cgtgcgtgct
     2341 cgcgtcgatc ttcgcccaca cccgatccgc cagcgcgccc gtgaagatca tcgtgactgc
     2401 gttgacgggg atgagcatcg cgatccccat ccctgccggc gacgggatga acggacgcca
     2461 actctccttc tgttccagcc acgccagcgc gatcccgatg agcgcgccga tggtcgccga
     2521 ccatgtgatc cacgagagcc gcgcgatcgc ctcggggctg gaagcgttcg gccctgacag
     2581 ctcctgcgtg acgagcttgg cgaagccgac ccatcgctgc gacgtcgggc tgagcagttg
     2641 cgcgttgtcg ccgacgatgc cgtaggtgtc gcgca
//
LOCUS       DBNGSH010000637         3934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3461471, whole genome shotgun sequence.
ACCESSION   DBNGSH010000637 DBNGSH010000000
VERSION     DBNGSH010000637.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3461471"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="AC16CY_14705"
     CDS             complement(<1..278)
                     /locus_tag="AC16CY_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR3355096.1"
                     /translation="MPRVYRAGDAARALVIVLVSFATCFGQDTSRMEQVVQEQLPGNR
                     FMGTVLVARGEKILLNKGYGYANLELEVANTPATRFRIGSITKQFTAAA"
     gene            complement(345..1091)
                     /locus_tag="AC16CY_14710"
     CDS             complement(345..1091)
                     /locus_tag="AC16CY_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355097.1"
                     /translation="MAGAVPRTPDGKPDLTGVWQGGNGRRGTWEEANSGLGVGGTGLD
                     PDAPPSLGSQQVITDPAPYQPWAAARVMESFKRRGIDDPAGLCLPLGVPRNTIVGLFP
                     IQIVQTPARIVILYEYMSVFRVIPIDAEHPADLVPTYMGDSVGRWEGDTLVVDVVGFN
                     DKGWLTSTGTFHSEALHVTERYTRIDKDQINLEVTMDDPKVLTQPWTIHTTLMHREGT
                     RLQEYVCAENNVEVERFQKILREGVPFTRP"
     gene            complement(1187..1612)
                     /locus_tag="AC16CY_14715"
     CDS             complement(1187..1612)
                     /locus_tag="AC16CY_14715"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355098.1"
                     /translation="MRRAFIVCLAAFGWLLASPAPAHHSFGAEYDGSKPITLKGVVTK
                     IEWTNPHSHFSLDVKDEKGRTVSWQLSGYTVNALYRTGWKKDVTMKVGDTVSVFGWLA
                     RDGSRSAHAREVTFANGKKLFYGPPAGTGEGQKPPVKVP"
     gene            complement(1665..2615)
                     /locus_tag="AC16CY_14720"
     CDS             complement(1665..2615)
                     /locus_tag="AC16CY_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3355099.1"
                     /translation="MVLVRVLTTAALMAWVVALAHAQASRTARDGVYTDAQAARGQSI
                     YGKVCASCHGADMEGAQGPPLTGGAFAGRWQNQPLSELASKIGNTMPPDAPGLSAQES
                     ADLVAHLLKAVGFPSGNTELPSEDAALSRIMWPEGAATDKGGAAAPARVYPPLGNMAQ
                     LMRGIFFPNSNLIFTVQTRDPGAPVPPPAPGAQSSGFSWVDWGAGIYGGWQLVDNAAI
                     ALADASPLMLTPGMRCENGRPAPVTDADWVKYTEEMIAVARKVYRASQSRKQEAVSDA
                     TGDLADACANCHRAYRDVRGPGRGLDPTDPSNKSARCRPR"
     gene            2820..>3934
                     /locus_tag="AC16CY_14725"
     CDS             2820..>3934
                     /locus_tag="AC16CY_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355100.1"
                     /translation="MSRLILVVVASALIAPPHLAAQQSSVLDAAAKALGTTGLRSLQF
                     TATGQAFVLGQPPTAAEPWPVRPIKSYQVSIDYGSNSMRVEQVLTMPTPQPRGGGAPF
                     AGEQRQVQFVRGAFSWNETPPAAGSQTPGIQPQPAAAVERILWMWAASPQGAIKGAAS
                     NARVRTVASGAEVSFTVAARYPVAALVNKANQVERVQATVANDVLGDMQIVTTYSGYK
                     DFGGILFPSRIVQTQGGYPTLDLTVTAVQTNQFVDITVPDAVRNAAAPAPPAATSQKV
                     ADGLFWITGGSHHSLAADMGDHVVVVEGPQNEARSEVVIAEVKKVIPNKSIRFVVNTH
                     LHFDHSGGLRTYVDEGATVVTHQGNIAFYEKAWAAPRT"
BASE COUNT          656 a         1297 c         1323 g          658 t
ORIGIN      
        1 gcggcggcgg tgaactgctt ggtgatcgat ccaattcgga accgcgttgc cggggtgttc
       61 gcaacctcca attcgagatt ggcgtacccg tagcctttgt tcagcaggat cttctcgcct
      121 cgggccacga ggaccgttcc catgaaacgg ttgcccggca gctgctcctg gacgacctgc
      181 tccatcctcg aggtgtcctg cccgaagcag gtggcgaagg aaacgagaac gatgacgaga
      241 gcgcgggccg cgtcaccggc cctgtagacg cgcggcatgt cgtgcctccg gcaagatcgt
      301 agagcggagg cttcagcctc cgcggactac gtgagtggat ggtgtcaggg cctggtaaag
      361 ggtacgccct ccctgaggat cttctggaag cgctcgactt cgacgttgtt ctcggcgcag
      421 acgtattcct ggaggcgcgt cccttcgcga tgcatcaacg tcgtgtggat ggtccacggc
      481 tgcgtcaaca ccttgggatc gtccatcgtc acctcgaggt tgatctggtc cttgtcgatg
      541 cgcgtataac gctcggtgac gtgcagcgcc tcggaatgaa acgtgccggt gctggtcagc
      601 cagcccttgt cgttgaagcc gaccacgtcc acgacgagcg tgtcgccctc ccatcgcccg
      661 accgaatcgc ccatgtaggt cgggacgaga tcggccgggt gctcggcgtc gatcgggatc
      721 acgcggaaca cgctcatgta ctcgtacaga atcacgatcc tggccggggt ttgtacgatc
      781 tgaatcggga agaggcccac gatcgtgttg cggggcacgc ccagcgggag gcacagtccg
      841 gccgggtcgt cgatgccgcg ccgcttgaac gattccatca cgcgcgcggc ggcccacggc
      901 tgatacggcg caggatcggt gatgacctgc tgcgagccga gactcggcgg cgcatcggga
      961 tcgagccccg tgcctccgac gcccagcccg gagttggctt cctcccacgt gccgcgccgg
     1021 ccgttgccgc cctgccagac gccggtcaga tccggcttgc cgtcaggggt gcgcgggacc
     1081 gcaccagcca ccgctgattt cgcaccgtcg ctcacggtcg gggcctgagc ggtacacgac
     1141 gcgcaccata accccgcgac gatcggtatc accagagcac gccgtgtcat ggcaccttca
     1201 ccgggggctt ctgcccctcg ccggtgccgg caggaggtcc gtagaacagc ttcttgccat
     1261 tggcgaaggt cacttcacgg gcgtgcgcgg agcggctgcc gtccctggcg agccagccga
     1321 agacgctcac cgtgtcgccg accttcatcg tgacgtcttt cttccatccc gtgcgataga
     1381 gcgcgttgac cgtgtagccg gagagttgcc agctgacggt gcgccccttc tcgtctttca
     1441 cgtcgaggga gaagtggctg tgcggattgg tccattcgat cttcgtgacg acgcccttca
     1501 gcgtgatcgg cttgctgccg tcgtattcgg cgccgaagga atgatgcgcg ggcgcggggc
     1561 tcgcgagcag ccatccgaac gccgccaggc acacgatgaa agcacgtctc atggcttcac
     1621 ctgtcgtaac tcaaaactca aaactcaaga ctcaaaactc actactaccg aggccggcat
     1681 cgcgccgact tgttcgacgg atcggtcggg tccagtccgc ggcccgggcc gcgcacgtcc
     1741 cggtatgcgc gatggcagtt cgcgcaggcg tcggccagat cacccgtggc gtccgacacc
     1801 gcctcctgct ttcgcgactg cgacgcccga tagaccttcc gcgcaaccgc gatcatctcc
     1861 tcagtgtatt tgacccagtc cgcatcggtc accggagcgg gacgtccgtt ctcgcaccgc
     1921 atgcccggcg tcagcatcaa gggggaggcg tcggcaagcg cgatcgccgc gttgtccacc
     1981 agctgccagc cgccgtagat gccagcgccc cagtcaaccc atgagaagcc gctcgactgt
     2041 gcgccgggag caggcggcgg caccggggcg ccagggtcgc gcgtctgcac ggtgaagatc
     2101 aggttcgaat tcgggaagaa gatcccgcgc atcagctgcg ccatgttccc gagcggtgga
     2161 tagacgcgag ccggggcggc cgctccgcct ttgtccgtag cggcgccttc gggccacatg
     2221 atgcggctca gcgcggcatc ctcggaggga agctccgtgt tgccggacgg gaagccgacg
     2281 gctttcagga ggtgggccac cagatccgcc gactcctgcg ccgaaaggcc gggagcgtcg
     2341 ggcggcatcg tgttgccgat cttgctggcg agctccgata gcggctggtt ctgccatcgg
     2401 ccggcgaagg cgcctccggt cagcggcggg ccctgggcgc cttccatgtc ggcgccgtgg
     2461 cacgacgcgc acaccttgcc gtaaatcgac tgaccgcgag ccgcctgcgc gtcggtgtag
     2521 acgccgtcgc gcgccgttcg cgaggcctgc gcgtgtgcca gagccacgac ccacgccatc
     2581 agcgcggccg tcgtcagcac cctgacgagc accatgatgc ctccggggtt ccgatgattc
     2641 gggagacagg atacatccgt acgaaggtgc gaaggtgcga gggtgcgaag gtgcgaaggt
     2701 gcgaaggtgc gagggtgcga gggtgcgagg gtgcgagggt gcgaaggtgc gaggaccgtc
     2761 atatactctc tccgtttttg ggaccggaga ccgtagaccg tagacggagg aggaactcca
     2821 tgtccagact catcctcgtt gtggttgcga gcgccctgat cgcgccgcca catctcgcgg
     2881 cgcagcaatc ttccgttctg gacgcggcgg ccaaggccct gggcacgacc ggcctgcgat
     2941 cgctgcagtt cacggccacg ggccaggcct tcgtgctcgg ccagccgccg accgcggctg
     3001 aaccctggcc ggtgaggccc atcaagagct accaggtcag catcgattac ggcagcaact
     3061 cgatgcgcgt cgagcaggtg ctgacgatgc cgacgccgca gccgcggggg ggcggagctc
     3121 ctttcgcggg cgagcagcgt caggtgcagt tcgtgagggg cgcgttctcg tggaacgaga
     3181 cgccgcccgc cgccggttcg cagacgcctg gcatccagcc acagccggcc gcggccgtcg
     3241 agcggatcct gtggatgtgg gcggcatccc cgcagggagc gatcaaaggg gccgccagca
     3301 acgcgcgggt tcgcacggtt gcgagcggcg cggaggtgag cttcacggtt gccgcgcggt
     3361 atcccgtggc ggccctcgtc aacaaggcga accaggtcga gcgcgtgcag gcgaccgtcg
     3421 ccaacgacgt cctcggcgac atgcagatcg tcaccaccta ctcgggctac aaggatttcg
     3481 gcggcatcct gtttccctca cgcatcgtcc agacgcaggg cggctatccc acgctcgacc
     3541 tgacggtcac ggcggttcag acgaaccagt tcgtggacat cacggtgccc gacgccgtcc
     3601 ggaacgcggc cgcaccggcg ccgccggcgg cgacatcgca gaaggtcgcc gacggtctct
     3661 tctggatcac cggcggatcg catcacagcc tcgccgccga catgggcgat cacgtggtcg
     3721 tcgtcgaagg gccgcagaac gaggcgcggt cggaggtggt gatcgccgaa gtaaagaagg
     3781 tgatcccgaa caagtcgatc cggttcgtcg tcaacaccca cctgcacttc gaccactcgg
     3841 gcggtctgcg cacgtacgtg gacgagggag cgacggtcgt cacgcaccag ggcaacatcg
     3901 cgttctacga gaaggcgtgg gcggcgcccc gcac
//
LOCUS       DBNGSH010000638         4356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3461492, whole genome shotgun sequence.
ACCESSION   DBNGSH010000638 DBNGSH010000000
VERSION     DBNGSH010000638.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3461492"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16CY_14730"
     CDS             complement(<1..461)
                     /locus_tag="AC16CY_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355101.1"
                     /translation="MTKRLAWPGGVLSLVLLSIACDVKVGEQGLSFDIAGGKVTEEWD
                     RTYTLKPGGRLEIVNLNGAIHASGSTDAEVQVHAVREVRASTDQAAQDLLKRFEIREE
                     VAADRVLIHVAEGEGGRGQARSRVTIRYEVRIPPGLDVLLRTQNGDVRMEGL"
     gene            complement(524..4318)
                     /gene="smc"
                     /locus_tag="AC16CY_14735"
     CDS             complement(524..4318)
                     /gene="smc"
                     /locus_tag="AC16CY_14735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR3355102.1"
                     /translation="MRLERLEISGFKSFSDRSELSFDQGVTAIVGPNGCGKSNVADAI
                     TWVLGEQSAKSLRGDKMEDVIFSGSDARKPGAAAEVRLKLAGLPVAPEPPASPGPALQ
                     EFHPFEGEGDQTAERPHGNGNGHGHPGIEELATAFTRDVEVTRRLYRSGESEYLIDGA
                     VCRLRDVHELLMDTGLGAKAYSIIEQGKIGMILSSRPTDRRQLIDEAAGITKYRSRRR
                     AAELKLEAAQQNLVRIDDIVFEVEKQRGALKRQAAKARRYGRLREEFRRWEKVLFAGR
                     YRELAAAIEGARARLSLAREREAAASARLAAVEADLGRLRIEQAEADGRTARLREDAH
                     TRELDLNLRQQQQEFNRQQVSGLDDRRTEIDGEIKELEERREPARVALDARRDAALEA
                     ERSRDEAAAVMASANAEHTRAQLDVSELEARVEVVRGDLFQSVNATMTVRHVTEHTSA
                     QRERVGEQLARLDVEQEDVRRERERVGADRAGVLDLEARARASLDAIALDLTTRRSQL
                     ASFRGEHEVCVRDVRTAEQELAAVEGRLASLEELAASRADFSDAARMVLVQANGRVGQ
                     QGAVADYLEVDRRYERAVEACLGDLLQHVIVERHDQAAAGLSLVREQDAGRCGFVVVD
                     PGSNGFHPRDAVRIPGIVPVSDVMRVHGPHATTILKVIPEAYVADTFEQAVAFSRQTF
                     APVATLDGDVLHGPHLVTGGAKAESRGILATRREIKEIRERVALDRAALDRLADLAAR
                     LEVSIAEATGAIAALQDAEHEQEKAIVAHEAQLARAEEEEVRLARKADVVAFERRRAD
                     EERAELEARRAEAESSIARLEGEQRMIEDRLAEAQRRLADGRETVGVLGAKAAEAGAT
                     HAALVERAAAIAADVLRLEEGATELEQRIAARHAEQQQNHTRRESLIAAIAAGERALD
                     DQVAALESMRGTLREADESAASLRSKVDEMDLGIREARRVLETVRAEASDLEVARATA
                     ESDLTHLARACVDAVQSSLDDVLAEVEQMEQSGQATPDAVALSAEEPDPEAEEIPAGA
                     APEAPAAGEAAIEEPQGPPLSMTAEDALVTLKAKIERLGPVNMMAIEQFDELDQRHGF
                     LTKQRQDLLDSIAQTTAAIERIDETTKARFTEAFSAIQANFHDTFSTLFGGGRAGLAL
                     LDENDPLESGIDIVASPPGKRLQNVQLLSGGEKALTAIALMFAIFKYKPSPFCLLDEI
                     DAPLDDANVHRFVQMLRGMLDRTQFILITHNRQTMEIANRLYGVTMEEPGVSKLISVQ
                     LN"
BASE COUNT          586 a         1594 c         1419 g          757 t
ORIGIN      
        1 agaccctcca tccgcacatc gccgttctgc gtcctgagca ggacgtccag gcccggcgga
       61 atccgcacct cgtagcggat cgtgacccgc gatcgcgcct ggccgcgccc tccctcgcct
      121 tcagccacgt ggatgagaac gcgatccgca gcgacctctt cgcgtatctc gaacctcttg
      181 agcagatcct gcgcggcctg atcggtggac gccctgacct cgcgcaccgc atgaacctgg
      241 acctcggcgt ccgtggaccc ggacgcgtgg atggcgccgt tcagattgac gatttcgagg
      301 cggccgcccg gcttgagcgt gtaggtccgg tcccactcct cggtgacctt cccgccggcg
      361 atgtcaaacg agaggccctg ctcgccgacc ttgacgtcgc aggcgatcga caacaggacg
      421 aggctgagca ctccgcccgg ccacgcgagt cgcttcgtca tctgtctgga cctccggact
      481 ttactacgtt cggacaggcc ggatcgttct gggcgaggcg tgctcaattc aactggacgg
      541 aaatcagctt cgagacgccg ggctcctcca tcgtcactcc gtacagccgg ttggcgattt
      601 ccatcgtctg gcggttgtgc gtgatcagga tgaactgcgt ccggtcgagc atcccgcgca
      661 gcatctggac gaacctgtgc acgttcgcgt cgtcgagcgg cgcgtcgatt tcgtcgagca
      721 ggcagaacgg gctcggcttg tacttgaaga tcgcgaacat gagcgcgatc gcggtcaacg
      781 ccttctctcc gcccgacagc agctgcacgt tctgcagccg cttgccgggg ggggacgcga
      841 cgatgtcgat gccgctttcc agcggatcgt tctcgtcgag cagagccagc cccgcgcgtc
      901 cgccgccgaa cagcgtgctg aaggtgtcgt gaaagttggc ctgaatcgcc gagaacgctt
      961 cggtgaaccg ggccttggtc gtttcgtcga tgcgctcaat cgccgcggtc gtctgcgcaa
     1021 tcgagtcgag caggtcctgg cgctgtttcg tcagaaaccc gtggcgctgg tcgagctcgt
     1081 cgaactgctc gatcgccatc atgttgacgg ggcccagccg ttcgatcttc gccttcagcg
     1141 tgacgagcgc gtcttcggcc gtcatcgaaa gcggcgggcc ctgcggctcc tcgatcgccg
     1201 cttcgccggc cgccggcgct tcaggggcag cacccgcggg gatctcttca gcctccggat
     1261 ccggctcctc ggccgacagc gcgaccgcgt cgggcgtcgc ctggccgctc tgctccatct
     1321 gctcgacctc ggccagcacg tcgtccaggg agctctgcac ggcgtccacg caggcccggg
     1381 caaggtgggt cagatcgctt tccgcagtgg ctctcgcgac ttcgagatcg ctcgcttcgg
     1441 cacgaacggt ctcgagcacc cgccgggcct cgcggattcc gagatccatt tcgtcgacct
     1501 tcgatcgcag cgacgccgcc gattcgtcgg cctctctcag cgtgccgcgc atggactcga
     1561 gcgcggcgac ctgatcgtcg agggcgcgct cccccgccgc gatggcggcg atgagcgatt
     1621 cgcggcgcgt gtggttctgc tgctgctcgg cgtgccgcgc ggcgatccgc tgctcgagct
     1681 cggtggctcc ctcctcgagg cggagcacgt cggccgcgat cgcggccgcg cgctcgacca
     1741 gcgccgcgtg cgtcgctccg gcctcggcag ccttggcccc gagcacgccg acggtctccc
     1801 gtccgtcggc gagccggcgc tgcgcctccg cgagccggtc ctcgatcatc cgctgctcgc
     1861 cctcgagcct ggcgatcgac gattcggcct cggcacggcg ggcttcgagc tccgcgcgtt
     1921 cctcgtcggc gcgcctccgt tcgaacgcga cgacgtccgc cttccgcgcc agacgcactt
     1981 cctcttcctc ggcgcgcgcc agctgcgcct cgtgcgcgac gatcgctttc tcctgctcgt
     2041 gctcggcgtc ctgcagcgcg gcgatggcgc cggtcgcctc cgcgatgctc acctcgaggc
     2101 gcgcagccag gtcggcgagc cggtcgagcg cggcgcgatc gagcgcgaca cgctcgcgta
     2161 tttccttgat ctcgcgcctg gtcgccagga tgccgcgcga ctcggctttc gcgccgccgg
     2221 tcacgagatg cggcccgtgc agcacgtcgc cgtcgagcgt agccacgggc gcgaacgtct
     2281 ggcgcgagaa cgcaacggcc tgctcgaacg tgtccgccac gtaggcctct gggatgacct
     2341 tgaggatcgt cgtggcgtgc gggccatgca cgcgcatcac gtcggacacc gggacgatgc
     2401 cggggatccg gacggcgtcg cgaggatgga acccgttcga gcctggatcg accaccacga
     2461 acccgcagcg gccggcgtcc tgttcgcgca cgagcgacag accagcggcg gcctgatcgt
     2521 ggcgctccac gatcacgtgc tggaggaggt cgccgaggca cgcctcgacc gcgcgctcgt
     2581 atcggcggtc cacttcgaga taatccgcga cggctccctg ctgcccgacg cggccgttgg
     2641 cctggacgag caccatgcgc gccgcgtcgc tgaaatccgc ccggctcgcg gccagctctt
     2701 cgagcgacgc caggcgtcct tcgacggccg cgagctcctg ctccgccgtc cgcacgtccc
     2761 tcacgcacac ctcgtgctca ccccggaacg aggccagctg cgaccgccgc gtggtgagat
     2821 cgagcgcgat cgcgtcgagc gatgcgcgcg cccgggcctc gagatcgaga acacccgccc
     2881 gatccgcgcc cacccgctcg cgctcccgcc ggacgtcctc ttgttcgacg tccagcctgg
     2941 ccagttgctc gccgacgcgc tcgcgctgcg cgctggtgtg ctccgtcacg tggcggacgg
     3001 tcatcgtcgc attgaccgac tggaagagat cgccgcgtac gacctcgacc cgggcttcga
     3061 gctcgctgac atcgagctgc gcccgcgtgt gctccgcgtt cgccgacgcc atgacggcgg
     3121 cggcctcgtc gcgcgaccgc tcggcctcga gtgccgcgtc acggcgagcg tcaagcgcca
     3181 cccgggcggg ctcgcgtcgc tcttcgagct ccttgatctc accgtcgatc tcggtccggc
     3241 ggtcgtcgag cccgctgacc tgctgccggt tgaactcctg ctgctgctgg cggagattga
     3301 ggtccagctc gcgcgtatgc gcgtcttcgc gtaggcgcgc cgtgcggcca tccgcctcgg
     3361 cctgctcgat gcgaaggcgt ccgaggtcgg cttcgaccgc cgcgagacgc gccgaggcgg
     3421 ccgcctcacg ttctctcgcg agcgacaacc tcgcccgcgc gccctcgatc gcggcggcca
     3481 gctcccggta gcggccggca aacagtacct tctcccagcg ccggaactct tctcgcaacc
     3541 gcccgtacct gcgggccttt gcggcctgcc tcttcaaggc gccccgctgc ttttccacct
     3601 cgaacacgat gtcgtcgatg cggacgagat tctgctgcgc cgcttcgagc ttcagctccg
     3661 cggcgcggcg gcgggaacga tacttcgtga tgcccgccgc ctcgtcgatc agctgacgcc
     3721 gatcggtcgg acgcgagctc aggatcatgc cgatcttgcc ctgctcgatg atcgagtagg
     3781 ccttggcgcc cagccccgtg tccatcagca gctcgtggac gtcgcgcagc cggcagaccg
     3841 cgccgtcgat cagatactcg ctctcgcccg agcggtacaa acgccgcgtg acctcgacgt
     3901 cgcgagtgaa ggcggtcgcc agctcctcga tcccggggtg tccgtggccg ttgccgttgc
     3961 cgtgcgggcg ttcagccgtc tggtcgccct cgccttcgaa gggatgaaac tcctgcaggg
     4021 ccgggccggg cgaggctgga ggctccggag caacgggcag gccagcaagc ttcaggcgga
     4081 cctcggccgc ggcgcccggc tttctggcgt cgcttccgct gaagatgacg tcctccatct
     4141 tgtcgccgcg gaggctctta gcgctttgct caccaagcac ccacgtgatg gcgtcggcga
     4201 cgttgctctt gccgcagcca ttggggccga cgatggcggt gacgccctgg tcgaaggaaa
     4261 gctccgaccg atcggaaaac gacttgaacc cggagatttc gaggcgttcg agacgcatta
     4321 cccttcctgc ccgacacgcg caccggaccc ggccgg
//
LOCUS       DBNGSH010000639         4488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1763557, whole genome shotgun sequence.
ACCESSION   DBNGSH010000639 DBNGSH010000000
VERSION     DBNGSH010000639.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1763557"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /gene="asnB"
                     /locus_tag="AC16CY_14740"
     CDS             <1..1861
                     /gene="asnB"
                     /locus_tag="AC16CY_14740"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3355103.1"
                     /translation="HRGPDDSGAWVDEQAGIALGFRRLAIIDVSPQGHQPMVSPSGRF
                     VIVFNGEIYNFKELRHDLEQGGCRFRGQSDTEVLLAACDRWGVQEAVRRSLGMFAFAL
                     WDVHRRELSLVRDRLGKKPLYVYREPGLVTFGSELKALVAGPSFDRSIDRTALASYLR
                     YLYVPAPRSIYARAIKVPPGHILTVSDPRCPLPATEPYWSLQEAARTGLSCPLDCSDA
                     EAVDSLDSLLGDAVRRRMYADVPLGALLSGGIDSSTIVALMQEASSRPVKTYTIGFDE
                     KEFNEAGHAARVAAHLGTEHTEVLLTGNDACALVPSLPEIFDEPLADPSQVPTLLVST
                     LARRDVTVALCGDGGDELFGGYNRYVHGTRMLPRLDRVPRGVRRRVGAGIGSVSTTTW
                     DRLHRVTSAVLPGIPSEHFGERVLKLGRVMSADSVGGMYRSLLSAWQRPGDVVVEDGP
                     DDDPNGRILDGRDPEDLLDRMMLADQAAYLPDDLLAKVDRASMAVSLEVRAPLLDHRV
                     VELSWRLPRSLKVRGPVGKWVLRQVLYRRVPREIVERPKMGFSVPIDRWLRGPLKSWA
                     SDLLTVNELAKDGLLNPAGVVDAWRGLQESGRPAGTALWAVVMFQAWRARWHA"
     gene            1897..3198
                     /locus_tag="AC16CY_14745"
     CDS             1897..3198
                     /locus_tag="AC16CY_14745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3355104.1"
                     /translation="MSERTGRPRLLFLCHTLPYPPDGGPWIRTFHVLRHLARAFDITA
                     LCFERSPTAGGGVAWDVAAGNEALGRYAAVEVFPIPQARSRSRFVWDHLRSTALAQVY
                     TTYLYQSGAFRRRLRQLLRSRSFDLVHIDSFDLSGYLPECHSLPVVCVHHDIQSALLR
                     RRAAVDARVLRRAYARYQAALMAREERRWCGRVGLNVAVSDVDAALLMRLVPAARVAV
                     VPNGVDVDEFQSSGDGTGTGVAFVGGAAVFPNAAALEFFCTAILPHLRAATTDLPVRW
                     IGRATPDQQRHYAGRYGVHLTGYVEDVRPLMREAACHVVPLTTGGGTRLKILNSWAMG
                     KPVVSTSIGCEGLAAVDGDNILIRDEPAAFADAVLRVLADETLRRRLGAGGRATVERL
                     YSWDIVGERMRNAYLDLAREHAQNLLSPCSAISGQLYPLHE"
     gene            3224..>4488
                     /locus_tag="AC16CY_14750"
     CDS             3224..>4488
                     /locus_tag="AC16CY_14750"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3355105.1"
                     /translation="MAPATHLARRADPSWDLLLICVAGYILTAVGRLHQLFPALQALR
                     PAIVTGLAAIGLYVIDRRQSRQPGLLWVPTTRYLLALLAWMALSLPGSIWPGNAFDLL
                     FDNFIKTFLMYLVLVGSVRGDRDVERLAFAYFLSAAVYAFVVLTRFDVGDGDAWRLGR
                     LYYYDANDFAAFAVSAMPFGLYFARTGRAVLTKVGAAAALALLIVAFVRSGSRGGFVA
                     LLVMGGYVVVRYTAIALRWRLAAVAIVVSVLAATASDEYWRQMGTILSDTDYNQTAES
                     GRLQIWRRGVGYMLQHPVLGVGANNFGVAEGTLSPMASRQQFGAGVRWNAAHNSFVQV
                     GAELGVPGLMLFIAIITTAFAALARPRRPTSVETGLAERPAELRQTLTASLLGFVVAA
                     FFLSLAYSEMLYTLVALAIAFGKVTDARAP"
BASE COUNT          617 a         1482 c         1554 g          835 t
ORIGIN      
        1 gcatcgcggg cccgacgaca gcggggcgtg ggtggacgag caggccggga tcgcactcgg
       61 gttccgccgg ctcgccatca tcgacgtatc tccgcaaggc catcagccga tggtgtcgcc
      121 ctccggccgg ttcgtcatcg tcttcaacgg cgagatctac aacttcaagg agctgcgtca
      181 cgacctggag cagggggggt gccgcttccg ggggcagtcg gacacggagg tcctcctcgc
      241 ggcgtgcgat cgatggggcg tccaggaggc ggttcgccgc tcgctcggca tgttcgcttt
      301 tgcgctgtgg gacgtgcatc gccgggagct gtcgctcgtg cgggaccgtc tcggcaagaa
      361 gccgctgtat gtgtatcgcg agccgggtct cgtcacgttc ggctcggagc tcaaggctct
      421 ggtggccgga ccgtccttcg accggtcgat cgatcgcacg gccctggcgt cgtatctgcg
      481 atacctctac gtccctgcgc cgagaagcat ctacgcgcgc gcgatcaagg ttccgccggg
      541 gcacatcctg accgtctcgg atccgcggtg cccgctgccc gcgaccgagc cgtactggtc
      601 cctgcaagaa gctgcgcgga cagggctgtc gtgtccgctc gactgctccg acgccgaggc
      661 ggtcgactcg ctcgactcgc tgctgggcga tgcggttcgc cggcggatgt acgcggacgt
      721 gccgctcggg gcgctgttgt cgggaggcat cgactcctcg accatcgttg cgctcatgca
      781 ggaagcgagc agccgtccgg tcaagaccta cacgattgga ttcgacgaga aagagttcaa
      841 cgaggccggg catgccgcca gggtcgcggc gcacctggga acggagcaca ccgaggtgtt
      901 gttgacgggc aacgacgcgt gcgcgctggt gccgtccttg ccggagatct tcgacgagcc
      961 gcttgccgac ccgtctcagg tgccgacgct gctcgtgtca acgctcgccc gcagggacgt
     1021 cacggtggcg ctgtgcgggg atggcggcga cgagctgttc ggcggctaca accggtacgt
     1081 ccacggcacc cgcatgctgc ctcgactcga ccgtgtgccg cggggtgtcc ggcgccgcgt
     1141 cggcgcgggc atcggcagcg tgtcgacgac gacatgggat cgcctgcacc gtgtcacgtc
     1201 ggccgtgctt ccgggcatcc cctccgagca tttcggggag cgggtgctga agctcggccg
     1261 cgtcatgagc gcggactcgg tgggcggcat gtatcgctcg ctgctgtcgg cgtggcagcg
     1321 gccaggcgac gtggtcgtgg aggacggccc ggacgacgat ccgaacggcc ggattctgga
     1381 cgggcgcgac ccggaggatc tgctcgatcg gatgatgctg gccgatcaag ccgcgtatct
     1441 gcccgatgac ctgctcgcca aagtcgatcg cgcgagcatg gccgtcagtc tggaggtccg
     1501 cgcgccgctt ctggatcacc gcgtcgtcga gctctcgtgg cggctgcccc gttccctcaa
     1561 agtgcgcggc ccggtgggga agtgggtgct gcgccaggtc ctctaccgtc gcgtgccgcg
     1621 cgagatcgtc gaacggccga agatgggatt ttcagtcccg atcgatcgct ggctgcgcgg
     1681 gccgctgaaa agctgggcca gcgatctcct gaccgtgaac gagctggcca aggacggact
     1741 gctcaatccg gcgggagtcg tcgatgcctg gagaggactg caggaatccg ggcgtcccgc
     1801 gggcaccgcg ctctgggccg tcgtgatgtt ccaggcgtgg agggcgcggt ggcacgcctg
     1861 agcactcgcc cgatcacatt cggaggtcac tgagccatgt cagaacgaac gggccgtcct
     1921 cgtctgctct tcctgtgcca cacgctgccg tacccgcccg acggcggacc atggatcaga
     1981 acgttccatg tgctccgtca cctcgctcgt gccttcgaca tcaccgcgtt gtgcttcgag
     2041 cgatcgccga cggcgggtgg aggagtcgcc tgggacgtgg cagccggcaa tgaggcgctt
     2101 ggacggtacg ccgctgtcga ggtcttcccg attccccagg cgcgcagtcg atctcgattt
     2161 gtctgggatc atttgcggag cacggcgctg gcgcaggtct atacgaccta cctgtaccag
     2221 tccggggctt ttcggcgtcg gctccgccag ctgctgcggt cccggtcttt cgatctcgtt
     2281 cacatcgaca gcttcgatct gtcgggctat ctgccggagt gccactcgct gccggtcgtc
     2341 tgcgtgcacc acgacatcca atccgcgctg cttcgccggc gcgctgccgt cgatgcccgc
     2401 gtgctgcggc gtgcctacgc ccgataccag gccgctctca tggcacggga ggaaaggcgc
     2461 tggtgcggcc gcgtcgggtt gaacgtggcc gtgtccgacg tggacgcggc tttactgatg
     2521 cggctcgtgc cggccgcgcg cgtggccgtg gtgccgaacg gcgtggacgt ggacgaattc
     2581 cagtcgtccg gcgacgggac ggggacgggc gtcgccttcg tcggtggggc cgccgtgttt
     2641 cccaatgccg cggctctcga gttcttctgc accgcgatcc tcccgcatct gcgggcggca
     2701 acgaccgacc ttcccgtccg gtggatcggg agggcgacgc ccgatcaaca gcgacactac
     2761 gccggccggt acggcgtcca cctgacgggc tacgtcgagg atgttcgacc gctgatgcgg
     2821 gaggccgcgt gccacgtcgt gccgttgacc accgggggcg gcacgcgact caagattctc
     2881 aactcgtggg cgatggggaa gcccgtcgtc agcacgtcaa tcggctgcga gggcctggcg
     2941 gccgtcgacg gggacaacat cctgatccgc gacgagccgg cggccttcgc cgatgccgtc
     3001 ctgcgcgtgc tcgccgacga aaccttgcgc cgcagattgg gcgccggcgg tcgggcgacg
     3061 gtcgagcggc tgtacagctg ggacatcgtc ggagaacgaa tgaggaatgc gtacctggat
     3121 ctcgcgcgcg agcatgctca gaatctcctc tccccttgct ccgccatcag cggacagctt
     3181 tacccgctac acgaatagcg cagtcagacc atccctgaca gcaatggcgc cggcgaccca
     3241 tctcgcgcgg cgcgccgacc cgtcgtggga tctgctgctc atctgtgtcg cgggctacat
     3301 cctgaccgcc gtgggccggc tgcaccagct gttcccggcg ctgcaggcgc ttcgtcccgc
     3361 gatcgtgacg ggattggccg ccatcgggtt gtatgtcatc gatcgacgtc aatccaggca
     3421 gccagggctg ctctgggtcc cgacgacgcg ctatctgctg gcgttgctgg cgtggatggc
     3481 cttgtccctc ccgggttcga tctggccggg caacgcgttc gatctgttgt tcgacaactt
     3541 catcaagacc ttcctcatgt atctcgtgct cgtgggctcc gtgcgcggtg accgggatgt
     3601 cgagcggctg gccttcgcgt atttcctgtc tgcggcggtg tatgccttcg tggtgctcac
     3661 gcgattcgac gtcggcgacg gcgacgcgtg gcgcctcggg cgtctctact actacgacgc
     3721 gaacgacttc gcggcgtttg ccgtctcggc gatgccgttc ggtctctatt tcgcgcgcac
     3781 cggacgcgcc gtgctcacca aggtgggcgc cgctgccgct ctggccctgc tgatcgtcgc
     3841 gttcgttcgc tctggatccc gagggggatt cgtggccctc ctggtcatgg gcggctatgt
     3901 cgtcgtgcga tacaccgcca tcgctcttcg ctggcggctc gccgccgtcg cgatcgtcgt
     3961 gtcggtgctc gcagccaccg cgagcgacga gtactggcgc cagatgggga cgattctttc
     4021 ggacaccgac tacaaccaga cggcggagag cgggcggctt caaatctggc gccggggcgt
     4081 cgggtacatg ctgcagcatc ccgtcctcgg cgtcggggcc aacaacttcg gcgtggccga
     4141 agggacgctg tcgccgatgg cttcgcggca gcagttcggc gccggcgtcc ggtggaacgc
     4201 cgcgcacaac agcttcgttc aggtcggggc cgagctcggc gtgccgggcc tcatgctgtt
     4261 catcgccatc atcacgaccg cgttcgcggc tctcgccaga ccgaggcgac cgacgagtgt
     4321 cgagaccggc cttgccgaac gcccggcgga gttgaggcaa acgctgacgg cgtcgctcct
     4381 cggcttcgtc gtggccgcgt tctttctgtc gcttgcgtat tccgagatgt tgtacacgct
     4441 ggtcgcgctg gcgatcgcct tcggcaaggt gaccgacgcg cgggcccc
//
LOCUS       DBNGSH010000640         3014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_745259, whole genome shotgun sequence.
ACCESSION   DBNGSH010000640 DBNGSH010000000
VERSION     DBNGSH010000640.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_745259"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="AC16CY_14755"
     CDS             complement(<1..199)
                     /locus_tag="AC16CY_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355106.1"
                     /translation="MRLIHTAGLVGVVFALGFAAARAQVPQDAQAPTLDQMRKAVADL
                     RGGRIPRPASYDTMTAEQKAFV"
     gene            398..1336
                     /locus_tag="AC16CY_14760"
     CDS             398..1336
                     /locus_tag="AC16CY_14760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355107.1"
                     /translation="MTRLIVLGVLISVGALSMSVAAVQQAPGRGAQQPAARVVGVERL
                     KGNLYVMTGGGGNSAVFITAAGVVVVDTKNPGWGQPLLDRIKTVTDRPVTMIINTHTH
                     GDHVSGNVEFPYSVEVVTHENTAKNMQEMRTPTGITPAAGAPANIFKDNNGHGLPRRT
                     FKESMTLGSGNDRIELHYFGRGHTNGDAFVVFPALGVMHAGDIFSGKNIPLLDANNGG
                     SGVEIGRTLAKAAALKNVEQIIPGHSTVMTVADLKEYARFNNEFAAAVRAAKKAGRTP
                     DEVAASWKIPAKYKGYGEPQAARLRANVQVVWDETK"
     gene            complement(1341..>3014)
                     /locus_tag="AC16CY_14765"
     CDS             complement(1341..>3014)
                     /locus_tag="AC16CY_14765"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006010945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR3355108.1"
                     /translation="ERRDANASEVVLDTRDLEIRQVEIGQNLGWTKTSFTLDAATAAF
                     GSALRIAMPRGADRVRITYSSSAAARGLQWLTPAQTAGKKHPFLFSQAQAINARSFIP
                     LQDTPGVRVTYDATVRVPKDLVAVMAAEMIPGDAGSGLFHFRMPQPIPSYLIALAVGD
                     LAFKPMSDRTGVWAEPSMVEAAAREFDDTEQMIKTTEALYGPYRWGRYDLLVLPPSFP
                     FGGMENPRLTFATPTVIAGDKSLVALVAHELAHSWSGNLTTNATWLDFWLNEGFTTYL
                     ERRIVEAVFGKRVADMQGVIALRDLEQSRKDLTVPGDRTLLPDLSGRDPDEAYSTVPY
                     EQGALFLSFLEARFGRQAFDQFLRRWFDEHAFQAVTTAQFLAFLNAHLMAPNPAAVTD
                     AQLQEWLHSETVPAFAVLPSSDALARVEAARDAWLGGGTIDALAKDAASWSTQEWMHF
                     VDSLPRKMDASRLGALDRRFRLTDSQNAEIAHVWFRLAIANRYASAYPAMERYMIQIG
                     RRKLIVPLYRDLAATRDGMALATRIYAKARDGYHPLAQGTIAGILKQRT"
BASE COUNT          517 a         1010 c         1031 g          456 t
ORIGIN      
        1 tgacgaaggc cttctgctcg gccgtcatcg tgtcgtagga cgcgggacgc ggaatccgcc
       61 cgccgcgcag gtcggcgacc gccttgcgca tctggtcgag cgtcggcgcc tgcgcgtcct
      121 ggggcacttg tgcccgggca gcggcaaagc cgagcgcaaa gacgactccg acgaggcccg
      181 cggtgtgaat cagacgcacg gctcccccct ttcgacggcg ccagccatga tactgccgtc
      241 acggtcgggc taaagccggc acgcacacgg tccggctgcc ttcgacttcg ctcaggccag
      301 ggaagccgga cactacgtac gacgctgacc cctgccccct gccccctgcc cccttccccc
      361 tgccccctgc tatgatccgg ccgctcgagg agggcccatg accagactga ttgttctcgg
      421 agtcttgatc tccgtgggtg cgctgtccat gtccgtcgcc gccgtgcagc aggcgccggg
      481 ccgcggcgct cagcagccgg cggcgcgcgt cgtcggagtc gagaggctca agggcaacct
      541 ctacgtgatg accggcggcg gaggcaacag cgcggtcttc atcacggccg cgggcgtcgt
      601 cgtggtcgac acgaagaacc cgggctgggg ccagccgctg ctcgacagga tcaagacggt
      661 gacggaccgc ccggtgacga tgatcatcaa cacgcacacg cacggtgacc acgtgagcgg
      721 caacgtcgaa ttcccctatt cggtcgaggt cgtcacccac gagaacaccg ccaagaacat
      781 gcaggagatg cgtacaccga ccggcatcac cccggccgcg ggcgcgccgg ccaatatctt
      841 caaggacaac aacgggcacg gcctgccccg gcggaccttc aaggaatcga tgacgctcgg
      901 cagcggaaac gaccggatcg agctccacta cttcggacgg ggccacacca acggcgacgc
      961 gttcgtcgtg ttcccggccc tcggggtgat gcacgccggc gacatcttct cggggaagaa
     1021 catcccgctg ctcgacgcca acaacggcgg cagcggcgtc gagatcggac ggacgctggc
     1081 caaggcggcg gcgctgaaga acgtcgaaca gatcatcccg gggcacagta ccgtgatgac
     1141 cgtggccgat ctgaaggagt acgcgaggtt caacaacgag ttcgccgcag ccgtgcgcgc
     1201 ggccaagaag gcggggcgca cccccgacga ggtggcggca tcgtggaaga ttccggcaaa
     1261 gtacaagggc tacggggaac cgcaggcggc acggctccgg gcgaacgtgc aggtcgtctg
     1321 ggacgaaacg aagtaagccg ttacgttctc tgcttgagga tgccggcgat cgtgccctgc
     1381 gcgagcgggt ggtagccgtc cctcgccttc gcgtagatcc tggtcgcgag cgccatcccg
     1441 tcgcgagtcg ccgccaggtc gcgatacaac ggcacgatga gcttccgccg gccgatctgg
     1501 atcatgtagc gctccatggc tggatacgcc gacgcgtagc ggttggcgat cgcgaggcgg
     1561 aaccagacgt gcgcgatctc tgcgttctgt gagtcggtca gcctgaagcg gcgatcgagc
     1621 gcgccaagcc ggctcgcgtc catcttgcgc ggcagcgagt ccacgaagtg catccactcc
     1681 tgcgtggacc aggaggccgc gtccttcgca agcgcgtcga tcgttccgcc gccgagccat
     1741 gcgtcccgcg ccgcttccac tctggcgagc gcatcggacg agggcagcac ggcaaaggcc
     1801 gggacggtct cgctgtgcag ccactcctgg agctgggcgt cggtaacagc cgccgggttg
     1861 ggcgccatca ggtgcgcgtt caggaacgcc aggaactggg cggtcgtgac cgcctgaaac
     1921 gcgtgctcgt cgaaccatcg ccgcaggaac tggtcgaacg cctggcggcc gaacctcgct
     1981 tcgagaaacg acaggaacaa ggcgccctgt tcgtagggca ccgtggagta cgcctcgtcc
     2041 ggatcacggc cgctcaggtc gggcaggagc gtccggtccc ctggaaccgt caggtccttt
     2101 cgcgattgct cgagatcacg aagggcgatg acgccctgca tgtcggcgac cctcttgcca
     2161 aagacggctt cgacgatgcg gcgctcgagg taggtggtga agccctcgtt cagccagaag
     2221 tccaaccacg tggcgttggt cgtcaggttc ccggaccagg agtgcgccag ctcgtgcgcc
     2281 acgagcgcga cgagcgactt gtcgccggcg atcaccgtcg gcgtggcgaa ggtgagccgc
     2341 ggattctcca tgcccccgaa cggaaacgac ggcggcagca cgagcaggtc gtaccgtccc
     2401 caccggtacg gaccgtacaa ggcctcggtg gtcttgatca tctgctcggt gtcgtcgaac
     2461 tcgcgcgccg ccgcctcgac catcgacggc tccgcccaga cgccggtccg gtcgctcatc
     2521 ggcttgaacg cgaggtctcc cacggcgagg gcgatgagat acgagggaat cggctgcggc
     2581 atgcggaagt ggaacaggcc gctgccggcg tcgccgggaa tcatctcggc ggccatgacg
     2641 gccaccagat ccttcgggac gcggaccgtg gcgtcgtagg tcacgcgcac gccgggcgtg
     2701 tcctggagcg ggatgaacga gcgcgcgttg atggcctgcg cctggctgaa gagaaacggg
     2761 tgcttcttgc cggcggtctg cgcgggcgta agccactgaa ggccgcgcgc ggccgccgat
     2821 gacgagtagg tgatccgcac acggtcggcc ccccgcggca tcgcgatcct gagcgcgctg
     2881 ccgaaggccg ccgtcgccgc gtcgagcgtg aagctggtct tcgtccagcc gaggttctgt
     2941 ccgatctcga cctggcgaat ctcgaggtcg cgcgtgtcga ggacgacttc ggacgcgttg
     3001 gcgtcccggc gctc
//
LOCUS       DBNGSH010000641         3798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_235490, whole genome shotgun sequence.
ACCESSION   DBNGSH010000641 DBNGSH010000000
VERSION     DBNGSH010000641.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_235490"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..193
                     /gene="rpmB"
                     /locus_tag="AC16CY_14770"
     CDS             2..193
                     /gene="rpmB"
                     /locus_tag="AC16CY_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR3355109.1"
                     /translation="MAKRCDVCNKGPVVGRTISHAHNVGPRRFEPNLQTVRAIVNGAT
                     KRIRVCTRCLRDGKVTKAA"
     gene            195..575
                     /locus_tag="AC16CY_14775"
     CDS             195..575
                     /locus_tag="AC16CY_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3355110.1"
                     /translation="MRELVTSPDAPKAMGAYSPAIKAGNLLFVSGQIPIDPATGALVD
                     GNISTQTEQVMRNLGALLRAAGVGFEHVVRTTVFLADMSEFAAMNEVYSRYVTDPPPA
                     RATVQVARLPRDVRIEVDAIAVLS"
     gene            complement(576..2729)
                     /locus_tag="AC16CY_14780"
     CDS             complement(576..2729)
                     /locus_tag="AC16CY_14780"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3355111.1"
                     /translation="MIRFETLLEKMRSYSPEADLELLRRAYVFSALEHKGQVRHSGEP
                     YLVHPLEVADSLVDMKLDVVAVAAGLLHDVVEDTLTTPERIAELFGAEIAHVVEGVTK
                     IGAIPFSSSEERQAENFRKMLLAMVDDIRVILVKLADRLHNMRTLHHLPEERRVKIAQ
                     ETIDIYAPIANRLGMSKVKNELEELAFKYLEPKAYELLKAKVEAKRKHAEGVIDGLTR
                     TLRARLEDAQIPMMTLDGRIKRLYSIHLKLKRQKIDLERVYDFVAVRVITQSVKDCYA
                     TLGIIHQTWSPVPGRFKDFIAMPRPNGYQSLHTSVISEHGFPFEVQIRTDEMHRRAEE
                     GIAAHWKYKEGRVGDSRDERYFQWMRQLLEVEQEVRDPQEFLQNLKIDLYPEEVYIFT
                     PKGEVRSLPRGATAVDFAYTVHTDVGHQCVGARINGRIVPLRSPLQNGDIVEIVTQTG
                     HKPSRDWLSFVVSSRARSKIKHVLQAEERTRSIELGRRLFEKEARRFGQNPATLLQSQ
                     ELTRFASEYGAKSPDELLAHIGYGKLSPRTVLQKAVPGQLREDSSVVSVVRRVLRSGT
                     GADKIKVRGTDDVMVFRAKCCNPIRGERIVGYITRGKGVSVHSFGCPNVVNLMFDPER
                     RIDVEWDSDGGDQPYIVRLTMQVEDRKGMLAEISAKISDINTNITNMEARTGDDQQAR
                     IEMTVEIRDVKHLEKVIKSIKSVHGVLGVERAARA"
     gene            3002..>3798
                     /locus_tag="AC16CY_14785"
     CDS             3002..>3798
                     /locus_tag="AC16CY_14785"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3355112.1"
                     /translation="MQKFSSRAAVGALAVALGFAAAGCNQIAMLRATMAFKDANQLYR
                     GQDYRAATVKYSEALDLCRGANPDCTDPRLDPAYFFLANSYDNQYRPARRGEPANDEL
                     LTKAIENYKKAAEVVEEPIIKKRAMEYLVAAFGPDKLNEPSEAVPILRRMIELEPNEP
                     SNYSYLSRIYEDNGDYEQAEQLLLKARDMKPNDAVVYVTLANFYNRQGEFTKTMEALH
                     ARADREPNNPEAFYTIAQFYWDKSVRDFTTSAADKVKFVLAGHEAIDT"
BASE COUNT          681 a         1210 c         1199 g          708 t
ORIGIN      
        1 catggcgaaa cggtgcgacg tctgcaacaa gggtccggtc gtcgggcgga cgatttccca
       61 cgctcacaac gtgggtccca ggcggtttga acccaacctt cagaccgtcc gagccatcgt
      121 caacggcgcg accaagcgga ttcgcgtctg cacgcgatgt ttgcgcgacg gcaaggtgac
      181 caaggcggcc tgacgtgaga gagctcgtca cgtctcccga cgcgcccaag gcgatgggag
      241 cctactcgcc ggccatcaag gccggcaatc tcctctttgt ttcggggcag attccgatcg
      301 accccgcgac gggcgctctc gtggacggaa acatctcgac gcagaccgaa caggtcatgc
      361 gcaacctcgg ggccctgctc agggccgcgg gcgtgggttt cgagcacgtc gtccggacga
      421 cggtcttcct cgcggacatg agcgagtttg ccgcgatgaa cgaggtgtac agccgctacg
      481 tcaccgaccc gcccccggct cgcgccaccg tccaggtcgc caggctgccg cgggacgtca
      541 ggattgaagt agacgcgatc gcggtactct cgtaactacg ctctcgcagc ccgttccacc
      601 cccagcactc cgtggacgct cttgatggac ttgatcacct tctcgaggtg cttcacgtcc
      661 cggatctcga ccgtcatctc gatgcgcgcc tgctgatcgt cgccggtgcg cgcctccatg
      721 ttggtgatgt tcgtgttgat gtccgagatc ttggcgctta tttccgccag cattcccttc
      781 cgatcctcga cctgcatcgt gagcctgacg atgtagggct ggtcgccgcc gtcgctgtcc
      841 cactcgacgt ctattcgccg ctccggatcg aacatcaggt tcacgacatt cggacagccg
      901 aatgagtgga ccgagacccc cttgccgcgc gtgatgtagc cgacgatccg ttccccgcga
      961 atgggattgc agcacttcgc gcgaaagacc atgacatcgt cggtgccgcg aaccttgatc
     1021 ttgtcggcgc ccgtccccga gcggagcacg cgccggacca cggacacgac cgacgaatct
     1081 tcgcgaagct gcccgggaac ggccttctgc aggacggttc gcggcgacag cttgccgtat
     1141 ccgatgtgcg cgaggagctc gtcaggggac ttggccccgt attcggacgc gaaccgcgtc
     1201 agttcctggc tctgaagcag cgtggccgga ttctggccga acctccgcgc ctccttctcg
     1261 aacagccgcc ggccgagctc gatgctgcgc gtccgctctt cggcctgaag gacgtgcttg
     1321 atcttgctgc gcgcccggga cgagacgaca aacgacaacc agtcgcggct cggcttgtgt
     1381 cccgtctgcg tgacgatctc gacgatgtcg ccgttctgca ggggcgagcg gaggggaacg
     1441 atccgcccgt tgatccgcgc gccgacgcac tggtggccga catcggtgtg aacggtgtag
     1501 gcgaagtcca cggcggtcgc tccgcgcggc agcgaccgga cctccccctt cggcgtgaag
     1561 atgtagacct cttccgggta cagatcgatc ttgaggttct ggaggaattc ctgcgggtcg
     1621 cgtacctcct gctcgacctc gagcagttgg cgcatccact ggaagtagcg ctcgtcgcgg
     1681 gaatccccga cgcggccctc tttgtacttc cagtgcgccg cgatgccctc ctccgctcgc
     1741 cgatgcatct cgtcggtacg gatctgcact tcgaacggga acccatgttc gctgatgacc
     1801 gaggtgtgca gcgattggta cccgttgggg cggggcatcg cgatgaagtc cttgaagcgg
     1861 cccggcacag gagaccatgt ctggtgaatg atgccgagcg tggcgtagca gtccttgacc
     1921 gactgcgtga tgacgcggac ggcgacgaag tcgtagactc gctcgagatc gattttctgg
     1981 cgtttgagct tcaggtgaat gctgtacagg cgcttgattc gcccgtccag cgtcatcatc
     2041 ggaatctgcg catcctcgag tcgggcccgg agcgtgcgcg tgaggccgtc gatgacgcct
     2101 tcggcgtgct tcctcttggc ctcgaccttg gccttcaaca actcgtacgc cttcggctcg
     2161 aggtacttga acgcgagctc ctcgagctcg ttcttgacct tgctcatgcc gaggcgattg
     2221 gcgatggggg cgtagatgtc gatcgtctcc tgcgcgatct tgacgcgcct ctcctccgga
     2281 agatgatgga gcgtgcgcat gttgtgcagg cgatcggcga gcttgaccag gatgacgcgg
     2341 atgtcatcca ccatcgccag cagcatcttg cggaaattct cggcctgccg ctcttcgctg
     2401 gatgaaaagg ggatggcgcc gatcttggtg acgccttcga ccacgtgcgc gatctcggcg
     2461 ccgaacagct ccgctatgcg ctctggcgtc gtcaacgtgt cttcgacgac gtcgtgcagc
     2521 agcccggcgg cgacggccac gacgtcgagc ttcatgtcaa ccagcgagtc ggcgacttcg
     2581 agcggatgca ccaggtacgg ctcgccggag tgacggacct gtcccttgtg ctcgagggcg
     2641 gaaaacacgt acgcgcgccg gaggagctcc aggtccgcct ccgggctgta gctccgcatc
     2701 ttttcgagca gcgtctcgaa tcgaatcatg aagaacggtg tctaaccagt acggccggtt
     2761 tctggagtgc cgaactcgtc gcgaggatgc cgctatcctc atgatagccg gacccttgac
     2821 tggccagaaa acggatcact atactggccg ttcgccgggg agggcgctcg ggaaacccag
     2881 tcctggcgcc gggcggagac tgtcgcgttc gcggagagcc gggcttggcc ggaccatcgt
     2941 cgtcatctac gtagtgtccg gcttcagccg gaccgtcgcc gggaacaggg agatcgttgt
     3001 catgcagaag ttttcgtcga gagcggcggt cggagcgctc gcggtggcac ttggcttcgc
     3061 ggcggccggc tgtaatcaga tcgcgatgct cagggcaacg atggcgttca aggacgcgaa
     3121 ccagttgtat cgaggccagg actaccgcgc cgccaccgtg aagtacagtg aggcgctgga
     3181 cctgtgcagg ggggcgaacc cggattgcac ggacccgagg ctggacccgg cgtacttctt
     3241 tctcgccaac agttacgaca accagtatcg tcccgcgcga cgaggcgagc cggccaacga
     3301 cgaactgctg accaaggcga ttgaaaacta caagaaggcg gcggaagtcg tcgaggaacc
     3361 gatcatcaag aaacgcgcca tggagtatct ggtggccgca ttcggtccag acaagctgaa
     3421 cgaaccctcc gaggccgtgc cgatcctccg ccggatgatc gagctggagc cgaacgagcc
     3481 gagcaactac agctacctct cgcgcatcta cgaggacaac ggcgactacg aacaggccga
     3541 gcagctcctg ctcaaggcgc gcgacatgaa gccgaacgac gccgtcgtct acgtcacgct
     3601 ggccaatttc tacaaccgcc agggcgaatt caccaagacg atggaggcgc tccacgcccg
     3661 ggccgaccgg gaaccgaaca acccggaggc gttctacacg atcgcgcagt tctactggga
     3721 taagtccgtt cgcgacttca cgacatcggc ggcggacaag gtgaagttcg tgcttgccgg
     3781 ccacgaggcc atcgacac
//
LOCUS       DBNGSH010000642         3349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3971172, whole genome shotgun sequence.
ACCESSION   DBNGSH010000642 DBNGSH010000000
VERSION     DBNGSH010000642.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3971172"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="AC16CY_14790"
     CDS             complement(<1..160)
                     /locus_tag="AC16CY_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR3355113.1"
                     /translation="MEFQDKSLRCVDCGSEFIWTAGEQLFFADKQFKNEPKRCKTCKA
                     KRANRASAG"
     gene            complement(203..400)
                     /gene="rpsU"
                     /locus_tag="AC16CY_14795"
     CDS             complement(203..400)
                     /gene="rpsU"
                     /locus_tag="AC16CY_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR3355114.1"
                     /translation="MAEVKIQEGESIESALRRFKRKVQQEDIIKDIKKHSFYLKPGDK
                     RRAKQALARKRNRKKLRRETE"
     gene            643..1932
                     /gene="purD"
                     /locus_tag="AC16CY_14800"
     CDS             643..1932
                     /gene="purD"
                     /locus_tag="AC16CY_14800"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR3355115.1"
                     /translation="MKILVVGGGAREHAIAWKLSRERSVTALVCAPGNPGIGAIAKCV
                     PADAGDPPALLDVARSEAIDLTVVGPELPLSRGVVDLFAADGRAIVGPSQAAAALESS
                     KAFAKNFMARHRVPTAAYRICDSGSAALAQLDRGELGYPVVIKADGLAAGKGVVIASD
                     RSAAEAAVKSIMLDRRFGAAGDRVVLEQFLTGQEASYFLLADGTSFVRLSSAQDHKRI
                     FDDDRGPNTGGMGAFAPSPLVTADLDRRIVEEIVRPVLEGMEDEGHPYRGFLYVGLML
                     TSDGPKVVEFNVRLGDPETQVVLPMLAEELSWLLGEAATGALPSRPASFSNEPHVGVV
                     LAARGYPENVEAGQPIFGMEEAAAIPGALVFHAGTKKREGRLVAAGGRVLTVVGRGAT
                     FGSAIETAYRAVERVRFEGMQFRRDIGRKAMGSGVIS"
     gene            1946..3208
                     /gene="mtaB"
                     /locus_tag="AC16CY_14805"
     CDS             1946..3208
                     /gene="mtaB"
                     /locus_tag="AC16CY_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR3355116.1"
                     /translation="MTPDPITYSVVTFGCRVNQADSLAIARDLRARGAIDRPAHQADV
                     VVVNTCSVTAAADQGARQTIRRLVRDNPRVQVVVTGCYATRCSDEVATLPNVVRVVVN
                     DDKDDLVERVARRLGLAGNPLVTTAARYGGGDGPCGDLSPGVAGRTALTLRVQTGCEQ
                     RCSYCIIPRTRGAGRSQPLSTVVRHLERAVAAGYREIAIAGVHLGSYGRDLGDGSCLT
                     TLVRTLAEWDADVLFRISSLEPMDCTPEIVELVATSPRLAPHFHLPLQHGSDEVLRTM
                     RRPYTANAYERLVARIRARVPDASIGSDLIVGFPGESDDHFAETEALLRRLPLTHLHV
                     FPYSDRPGTEAASMPGKVEGVTIRERGRVLRSIGQEMAARFRRSQHGTVRRALTVDDG
                     WSAVTDNYLKVKLPQRHERNEWVDARID"
BASE COUNT          540 a         1079 c         1090 g          640 t
ORIGIN      
        1 gaccggcact ggcccggttg gctcgcttgg ccttgcacgt cttgcaacgc ttcggctcgt
       61 tcttgaactg cttatctgcg aaaaacagtt gttcacctgc agtccagata aactcggagc
      121 cgcagtccac gcacctcaag gatttgtctt ggaactccat cggtcgccac ctcgtgctcg
      181 tcgatcgcgg gaccgctcgt atttactccg tttctcgacg gagtttctta cggttccgct
      241 tacgcgccaa ggcctgcttg gcccggcgct tgtcacccgg cttcaagtag aaagaatgct
      301 tcttgatgtc cttgatgatg tcttcctgct gaaccttgcg cttgaagcga cggagcgcgc
      361 tttcgatcga ctcgccctcc tgaatcttga cctcggccac gcctagatac accccctcat
      421 cggctcggtg gctccctgtg agaaaccttt gaaaagtgat gaatgtgtta ggctatcaca
      481 ggcaggcgcg gattctaggg cgcaacacgg catcacgtca acgaaatagt cgcgcgtaac
      541 aatctcacgt tgcacgagac gtggcgcccg actccggtgc gaggccgaaa ctcgccgccg
      601 tgcctgtcaa ccccctgtcc agcaggcagt tagcctgatt ccatgaagat cctcgtcgtg
      661 ggcggcggag ccagagagca cgccatcgcc tggaagctct ctcgcgagcg gagcgtgacc
      721 gccctggtct gcgcacccgg caatcccgga attggcgcca tcgccaagtg tgttcctgcc
      781 gatgcgggcg accccccggc gctgctcgac gtcgcgagaa gcgaagcgat cgacctgact
      841 gtcgtcgggc ccgagcttcc tctcagccgt ggcgtcgtcg atctgttcgc cgctgatggc
      901 cgggcgatcg tcgggccgtc gcaggctgcc gcggcactcg agtcgagcaa ggctttcgcg
      961 aaaaacttca tggcgcgtca tcgcgttccg accgctgcct atcgcatctg cgactcgggc
     1021 agcgccgcgc tcgcgcagct cgaccggggc gagctcggct acccggtcgt catcaaggcc
     1081 gacggtctcg cagccggcaa gggcgtcgta attgcgtccg accgctcggc ggccgaggcg
     1141 gcggtgaaat cgatcatgct cgaccgtcga ttcggggctg ccggcgaccg tgtcgtgctc
     1201 gagcagttcc tcacgggcca ggaggcttca tacttcctcc ttgccgatgg cacttcgttc
     1261 gtacggctct cgtcggcgca ggaccacaag cggatcttcg acgacgatcg agggccgaac
     1321 acgggaggca tgggcgcctt cgcaccgagc ccgctcgtca cggcggatct cgaccgtcgc
     1381 atcgtcgagg agatcgtgcg gccggtgctc gagggcatgg aggacgaggg acatccgtac
     1441 cgcgggtttc tctatgtagg cctgatgctc acctccgatg gtccgaaggt cgtcgagttc
     1501 aacgtgcgac tcggcgatcc ggaaacacag gtcgtgctgc cgatgctggc ggaggagttg
     1561 tcgtggttgc tgggcgaagc cgcgactggc gcgcttccct cgaggccggc ctcgttttcg
     1621 aacgaacctc atgtgggcgt cgtgcttgcg gcgcgaggct atccggaaaa cgtcgaggcc
     1681 ggccagccca tcttcggcat ggaagaggcg gccgccattc ctggtgcgct tgtttttcac
     1741 gccggcacga agaagcggga gggacggctt gttgccgcgg gcgggcgtgt gctgacggtc
     1801 gtcgggcgcg gggcgacatt cggcagcgcc atcgagacgg cgtatcgtgc cgttgaacgc
     1861 gtccgcttcg aaggcatgca gttcaggcgc gacatcgggc gcaaagcgat ggggtcggga
     1921 gtcatttcct gaggccagtc ggaaaatgac tcccgacccc attacatatt cggttgtcac
     1981 cttcggttgc cgcgtgaatc aggccgactc gctcgcgatc gcgcgcgatc tccgggcgcg
     2041 cggcgccatc gatcgaccgg cccatcaggc cgacgtggtg gtggtcaata cgtgctcggt
     2101 gacggccgcg gccgatcagg gagctcgcca gacgatcagg cggctcgtcc gcgataaccc
     2161 gcgcgtgcag gtcgtcgtga cggggtgcta tgccacccgg tgttctgacg aagtcgcaac
     2221 ccttcccaac gtcgtgcggg tcgtcgtgaa cgacgacaag gacgatctcg tcgagcgtgt
     2281 ggcgcgtcgc ctcgggctcg cggggaatcc actcgtgacg acggccgcac gctacggcgg
     2341 cggggacggg ccgtgcggag acttgtctcc cggcgtggcc ggccgcacgg ccctgacgct
     2401 tcgagtgcag acggggtgcg agcaacgatg cagctactgc atcatccccc ggaccagggg
     2461 cgcgggacga tcgcagccgc tctccaccgt cgttcgccac ctcgagcgcg cggtcgccgc
     2521 cggttatcgg gaaattgcca tcgcaggcgt ccatctcgga tcgtacggcc gcgatctcgg
     2581 tgatggttcg tgcctcacca cgctcgtccg cacgctggcc gaatgggacg ccgacgtgct
     2641 gtttcgaatc agttctctcg agccgatgga ttgcacgccg gaaatcgtcg aactggtggc
     2701 gacgtcgccc cggctggctc cccacttcca tcttccgctg cagcacggct cggacgaggt
     2761 cttacgtacg atgcggaggc cgtacacggc gaatgcctac gaacggctcg tcgcgaggat
     2821 tcgcgctcgc gtgccagatg cctcgatcgg gtcggatctc atcgtcggat ttcccggaga
     2881 gtcggacgac cacttcgccg agacagaggc tcttcttcgt cgtctcccgc tcactcactt
     2941 gcacgtgttc ccgtattcgg accggccggg aactgaagcc gcctcgatgc cgggaaaggt
     3001 ggagggcgtc accatccgcg aacgcggacg cgttcttcgt tcgattggac aggagatggc
     3061 tgcccggttc cgccgatcgc agcacggaac cgtccgtcgc gcgctgaccg tggacgacgg
     3121 gtggtccgcc gtgaccgaca actatctcaa ggtgaagttg ccgcagcgtc acgagagaaa
     3181 cgagtgggtc gacgcgcgca tcgactgacg tcgttcgttg gaagttggga ggttcttgat
     3241 tagcgcgtgc cgagtccctg tcatgcgagc caacagcccc gtgcccgtcg acacgctggc
     3301 gtgtccatcc gccggcgggt cgcggcgccc ggccatcggc atcgtcggc
//
LOCUS       DBNGSH010000643         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1424254, whole genome shotgun sequence.
ACCESSION   DBNGSH010000643 DBNGSH010000000
VERSION     DBNGSH010000643.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1424254"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="AC16CY_14810"
     CDS             complement(<1..1613)
                     /locus_tag="AC16CY_14810"
                     /inference="COORDINATES: protein motif:HMM:NF012700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS-related protein"
                     /protein_id="HIR3355117.1"
                     /translation="MAHPAEQYRSRLEARRATHRRLSVLDRRFSHARLGVFGAGALLA
                     LLAWQDALSTWLLLLPVAAFVVLIRRHELVIRARDSAARAITFYERGLARIEDRWPGT
                     GEAGDRFRDDRHFYANDLDLFGHASLFELLSIARTLAGERTLAGWLKAPAAPSEIAVR
                     QEGVAELTPALDLREALSLAGTDVRAGVHADNLAAWAESPRILTPPWVRWLLAAMTAI
                     VTVTVINWLAGGSELPLLVALAVQVAFAGPMQRRVDRALHQAAAAARDLDILRHLLER
                     LEREQFASERLVALRRAIAAEDGRASSAIRALHRLVEFHDWQHNLIFAIVSVLFLWGT
                     HIAWAIEAWRSRHGRHIRGWLDGVAEFEAFSSLSAYRFEHPGDPFPQIVSPDGAGPSA
                     VFDGVGLGHPLLPATRSVPNDVRLDKDQGLLVVSGSNMSGKSTLLRTVGLNTVLALAG
                     APVRARALRISPLAVGATLRIQDSLLEGRSRFYAEITRIRELADLSLGPRPLLFLLDE
                     LFHGTNSHDRLVGASGVLRSLLDRGAIGL"
     gene            1709..>2540
                     /locus_tag="AC16CY_14815"
     CDS             1709..>2540
                     /locus_tag="AC16CY_14815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3355118.1"
                     /translation="MSRNAIRGATAADIAASIERALQAGAFREEGRLPSIRSLSASLK
                     VSPVTVAAAYKRLHDRGLARGQGRGGTRLRPGPAGLTSSARPALLDERLVDLATGNPD
                     PALLPSLTGALRTVGEHHHLYGGPPDLPALAAFAAGEFAADGVPSGPICVTSGGLDAI
                     ERVVREHTRAGDRVAVEDPTFPALLDLLASLGVTPVPCPVDEEGPRPDSFARALGQGV
                     RVAIVTSRAHNPTGAAITESRAADLSRLLRPRPEILLLEIDDAGAVSGAPLVTLTGGR
                     P"
BASE COUNT          410 a          899 c          865 g          366 t
ORIGIN      
        1 atgaggccga tcgcgcctcg atcgagcagg cttctcaaca cacccgaggc gccaacgagc
       61 cggtcgtgcg agttggtgcc gtgaaacagc tcgtcgagca ggaacagcag cggccggggt
      121 ccgagcgaca ggtctgccag ctcgcgaatt cgcgtgatct cggcgtagaa acgcgatcgc
      181 ccctcgagca gcgagtcctg tattcgaagc gtcgcgccga cggccagcgg tgagatccgc
      241 agcgcacgcg cgcggaccgg ggccccggcg agcgcaagga ccgtgttgag gccgaccgtc
      301 cgcagcagcg tgctcttgcc cgacatgttc gacccgctca ccacgagcag cccctggtcc
      361 ttgtcgagcc gcacgtcgtt cggcaccgac cgcgtggccg gcaacagcgg gtggccgagc
      421 ccgacgccgt cgaacacggc cgaaggcccg gcgccatccg gcgagacaat ctgcggaaac
      481 ggatcgccag ggtgctcgaa ccggtaggcc gagagcgacg agaacgcctc gaactcggcg
      541 accccgtcga gccatccccg gatgtggcgg ccgtgccggc tccgccacgc ttcgatggcc
      601 caggcgatgt gcgtgcccca caggaacagg acggacacga tcgcgaagat caggttgtgc
      661 tgccagtcat ggaattcgac gagccggtgc agcgctcgaa tcgcagacga ggcgcggccg
      721 tcctctgccg caatcgcgcg acggagcgcg accaggcgct cggaggcaaa ttgctcgcgc
      781 tcgagccgct cgaggaggtg gcgcaggatg tcgaggtcgc gagccgccgc agcggcctgg
      841 tgcagggctc ggtcgacgcg gcgctgcatc ggccccgcaa aagcgacctg aacggccagg
      901 gccacgagca gcgggagttc gctgccgccg gcgagccagt tgatcaccgt cacggtgacg
      961 atcgccgtca tcgcggccag gagccaacgt acccagggcg gcgtgagaat cctcggggac
     1021 tcggcccagg cggcgagatt gtccgcgtgc acccctgctc tgacatcggt gccggccagc
     1081 gacagcgctt cgcgcaggtc gagcgccggc gtgagctccg ccacaccctc ctggcgtacg
     1141 gcgatttccg atggcgccgc cggcgccttc aaccagccgg ccagggtgcg ttctcccgcc
     1201 agcgttcgtg cgatcgagag cagctcgaag agcgacgcgt ggccgaacag gtcgagatcg
     1261 ttggcgtaga aatgccggtc gtctcggaag cggtcgccgg cttcgccggt tccgggccac
     1321 cggtcctcga tgcgagccag gccccgctcg taaaacgtga tggccctcgc cgcggagtcg
     1381 cgcgcgcgaa tcaccagctc gtgccgccgg atcaggacga cgaacgccgc gacgggaagc
     1441 aggagcagcc aggtgctcag cgcgtcctgc catgcgagca gcgccaacag ggcgcctgca
     1501 ccaaacacgc cgagacgggc gtggctgaac cgcctgtcca ggacagacag acggcggtgc
     1561 gtggcgcgac gcgcctcgag tcggctccgg tactgctcgg ccgggtgagc cacgggctct
     1621 attctagagc ctcgttttcc gagcctcact atccggcgac gccattgatt cgaccgtcaa
     1681 tatgttacag tacaactatt aaaataacat gtcacgaaac gccatccgcg gggccaccgc
     1741 cgccgacatc gcggcatcga tcgagcgcgc cctccaggcg ggcgcgttca gagaagaggg
     1801 ccggcttccc tcgatccgtt cgttgtccgc cagcctcaaa gtcagccccg tcaccgttgc
     1861 cgcggcctac aagcggctcc acgatcgcgg gctggcgcgt gggcagggcc gaggcggaac
     1921 acggctccgg cctggtcctg ccgggctgac gtcgagtgcc cggcctgcgc tcctcgacga
     1981 gcggctcgtc gatctcgcga ccggcaaccc cgaccccgcg cttctgccat ctctcaccgg
     2041 cgcccttcga acggtgggcg agcaccacca cctttacggt ggcccgccgg acctgcccgc
     2101 gcttgcggca tttgcggcag gtgaattcgc ggccgacggc gttccttctg gcccgatctg
     2161 cgtgacgagc ggcggcctcg acgccatcga gcgcgtggtg cgcgagcaca cgcgcgccgg
     2221 cgatcgggtc gccgtcgagg atccgacgtt tcccgcgctg ctcgacctgc ttgccagtct
     2281 tggcgtgacg cccgttccct gccccgtgga cgaggaaggg ccgcggccgg actcgttcgc
     2341 ccgcgcgctc gggcagggag tgcgggtggc gatcgtgacg tcgagagccc acaacccgac
     2401 cggcgcggcc atcaccgagt cgcgcgccgc cgatctttcg cgtctcctcc gccctcgccc
     2461 cgagattctt cttcttgaaa tcgacgacgc cggcgcggtg tctggagcgc ccctcgtgac
     2521 gttgacggga ggccggccga
//
LOCUS       DBNGSH010000644         3120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4311011, whole genome shotgun sequence.
ACCESSION   DBNGSH010000644 DBNGSH010000000
VERSION     DBNGSH010000644.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4311011"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="AC16CY_14820"
     CDS             complement(<1..748)
                     /locus_tag="AC16CY_14820"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF039614.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR3355119.1"
                     /translation="MTEYGRLSSVVVKHARDGFVDEQTIAAQWKALNYTDAPSLSRAI
                     DEHDRFVDILRSAGAEVHLLPRNDRTTLDSIYVRDASISSPRGLILCGMGKPQRAGEP
                     DAQDQALRTLGIHVAGRIAAPGRLEGGDLIWLDEVTLAVGRGYRTNAEGIRQLRALVG
                     DGVEVVEVPLPHWRGAGDVMHLMSLISPVDKDLAVVYSPLLPVPFREWLQDRGVRFVE
                     TPHEEFDTMGTNVLALGPSRCLTLAGNPLTR"
     gene            complement(745..1548)
                     /locus_tag="AC16CY_14825"
     CDS             complement(745..1548)
                     /locus_tag="AC16CY_14825"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355120.1"
                     /translation="MSRVTALVAKELADLRRNPGVFVPAAITGFASLLVPFFVGLIVP
                     VITGERLSDSSDFQVAAEMYRLEPATVRLSPEGAIQAWIFQQFLVMLILTPVAAAMSV
                     AAFSVIGEKQARTLEPLLVTPITTVELLTGKVLGAAIPALALGFGVFAVYAIGVAVFA
                     EAGVWRVLFLPRSLAVTFLLGPLASLASLQLAVCVSSRANDPRSAQQIGVLILLPIAG
                     ILAAQLLGSLQLTLGISVLIAVGLTMVNLALIVLGVRVFDRESILTRWR"
     gene            complement(1545..2483)
                     /gene="ccmA"
                     /locus_tag="AC16CY_14830"
     CDS             complement(1545..2483)
                     /gene="ccmA"
                     /locus_tag="AC16CY_14830"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR3355121.1"
                     /translation="MPFVVAHVLSAHHLTRRFGDRVAVEDVSFDLVAGEIFALLGPNG
                     SGKTTTLRMLAGLIAPSAGSISVQGERLTQESAPRLRRDIGFLTEAPGLWDRLTVRQN
                     LMVFARLHGLAQPHRAVDEALELFEMESRAGDRAAHLSKGLKQRVALARALLHDPRIV
                     LLDEPTAGLDPGSAHDVRELILRLRDRQRVVLLSTHNLDEAQRVANHVAILRTRLIAV
                     DTPAALKARLFEPRVRIVLTEPSQRFATAIQAAGAEDVQCDGVEMSVRVNGRLDTPAL
                     VRHLVQAGAGIESVVRVEPSLEDVYLKLVHERRGSS"
     gene            complement(2503..>3120)
                     /locus_tag="AC16CY_14835"
     CDS             complement(2503..>3120)
                     /locus_tag="AC16CY_14835"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3355122.1"
                     /translation="MRRQGPEREALPQIFVPLAQNPPPRNVDLFIRTSSEDPLAMAVA
                     LRAAARRIEKNAPIYGVAPLEQELGTYLTQRRFQTSLLTGFSVVALLMAAVGIYALIQ
                     YSVATRTRELGLRMAIGAQRGDIFRMMIGEGLTLSLASVALGLVGAWWLGRAGSSLLF
                     GVTASDPLTFATVSLLLTAVAMAACYFPARRATTIDPIVALRLTS"
BASE COUNT          567 a         1027 c         1041 g          485 t
ORIGIN      
        1 ggcgcgtcag cggattgccc gccagcgtca ggcatcggct cggcccgagc gcgagcacgt
       61 tggtacccat cgtgtcgaac tcttcgtggg gcgtttcgac gaaccgcaca ccgcgatcct
      121 ggagccattc gcgaaaggga accggaagca gaggcgagta gacgaccgcc aggtccttgt
      181 ccacggggct gatgagcgac atcaggtgca tcacgtcgcc ggcacctcgc cagtgaggca
      241 gcgggacttc cacgacttcg acgccgtcgc cgacgagcgc tcgcagctgc cggatgcctt
      301 ccgcgttcgt ccgatagcct cgtccaacgg caagcgtcac ctcgtcgagc cagatcaggt
      361 cgccgccctc gagacggccg ggggcggcaa tccggcctgc gacgtggatt ccaagcgtgc
      421 gaagcgcctg gtcctgcgca tccggttcgc cggcgcgctg cggctttccc atgccgcaca
      481 ggatcagccc gcgcggagac gagatagagg cgtcgcgcac gtagatcgaa tcgagcgtgg
      541 ttcgatcgtt gcgcggcagc aggtggacct cggcgcctgc cgatcgcagg atatcgacga
      601 aacggtcgtg ctcgtcgatc gcgcgcgaca ggctcggcgc gtccgtgtag ttgagtgcct
      661 tccactgggc cgcaatcgtc tgctcgtcaa cgaacccgtc gcgcgcgtgc ttgacgacga
      721 ccgacgacag gcggccgtac tccgtcatct ccacctcgtg agaatcgatt cgcggtcgaa
      781 gactcttacg cccagtacta tcagcgccag attcaccatc gtcagcccga cggcgatgag
      841 gacggaaatc ccaagtgtca actgcaggct tccaagaagc tgggcggcga ggatcccggc
      901 aatcggcaac aggatgagga cgccgatctg ctgcgcgctg cgtggatcgt tcgcgcgtga
      961 ggagacgcag acggcaagct gaagcgaggc gagcgacgcc aaaggcccaa gcagaaacgt
     1021 caccgccagc gagcgcggca ggaacagcac gcgccacacg ccggcttcgg caaacacggc
     1081 gacgccgatc gcgtagacgg cgaacacgcc gaatccgagg gccagggcgg gtatcgccgc
     1141 gccgaggacc ttgcccgtca gcagttcgac ggtcgtgatc ggtgtgacca gcagcggttc
     1201 cagcgtgcga gcctgtttct cgccgatgac gctgaaggcc gccaccgaca tcgcggcggc
     1261 cacgggcgtc aggatgagca tcaccaggaa ctgctggaag atccacgctt gaatcgctcc
     1321 ctcaggactg agtctcactg tggcgggctc caggcgatac atctcggcgg cgacctggaa
     1381 gtcgctcgag tccgacagtc gctcgcccgt gatcacagga acgatgagcc cgacgaagaa
     1441 gggaacgagc agcgacgcga atcccgtgat cgcggcaggt acgaagacgc cggggttcct
     1501 gcgcaggtcg gcaagctcct tggcgacgag ggccgtgacg cggctcatga ggatcctcgc
     1561 ctctcgtgca ccagcttcag gtacacgtcc tcgagcgacg gctcgacgcg gacgaccgat
     1621 tcgatgcccg caccggcctg aaccaggtgc cggacgagcg ccggagtgtc caggcggccg
     1681 ttgacccgga cggacatctc gacgccgtcg cactggacgt cttccgcgcc ggccgcctgt
     1741 atcgccgtgg cgaatcgctg tgacggttcc gtcaggacga tgcggacgcg aggctcgaag
     1801 agtcgcgcct tcagcgcggc tggcgtatcg accgcgatca ggcgcgtgcg caggatcgcg
     1861 acgtggttcg ccacccgttg tgcttcgtcg aggttgtgcg tggagagcag gacgacgcgc
     1921 tgccgatcgc gcagccggag gatcaattcg cggacgtcgt gggcgctccc ggggtcgagg
     1981 ccggcggtcg gttcgtcgag cagcacgatt cgcggatcgt gcaacagcgc tctcgccagc
     2041 gccaccctct gtttcagccc tttggagaga tgcgccgccc gatcgccggc gcgcgactcc
     2101 atctcgaaca gttcgagcgc ttcgtccacg gcccggtgcg gctgtgccag tccgtggagc
     2161 ctggcgaaca ccatcaggtt ctggcgtacc gtcaggcggt cccacagccc cggcgcttcc
     2221 gtgagaaagc cgatgtcgcg tcgaagccgg ggtgccgact cctgcgtgag cctctcgcct
     2281 tgcaccgata tcgagcccgc ggagggtgcg atgaggcccg ccagcatccg aagagtcgtg
     2341 gtcttccccg atccgttcgg tccgagcaag gcgaagattt ccccggccac caggtcgaac
     2401 gacacgtctt cgaccgccac gcgatcgccg aaacgccggg tgagatggtg ggcgctcagc
     2461 acgtgtgcta ccacgaaggg cacgagggtt gagccacgaa ggtcacgaag tcagacgtaa
     2521 tgccacgatc ggatcgatcg tcgttgcacg tcgagctgga aaatagcatg ctgccatggc
     2581 aactgccgtc agcaacagcg agacggtcgc gaacgtcaac ggatcgctcg ccgttacgcc
     2641 aaacagcaga ctcgagcccg cgcgcccgag ccaccaggct cccacgagcc cgagcgcgac
     2701 actggccagg ctgagcgtca gcccctcacc gatcatcatc ctgaagatgt cgcctcgctg
     2761 tgcgccgatg gccatgcgca agccgagttc ccgtgtgcgc gtcgcgacgg agtactggat
     2821 gagcgcgtag atcccgacag cggccatgag cagcgccacg actgagaagc cggtcaggag
     2881 cgaggtctgg aagcgacgct gcgtcaggta ggttccgagt tcctgttcga gaggcgccac
     2941 tccgtagatc ggcgcgttct tctcgatgcg acgcgcggcc gctcgaagtg ccacggccat
     3001 tgccagcggg tcctccgacg aggttcgtat gaagagatcc acgttccgcg ggggaggatt
     3061 ctgcgcgagc ggcacgaaga tctgcgggag cgcttcgcgc tccggcccct gccgccgcat
//
LOCUS       DBNGSH010000645        13403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4480929, whole genome shotgun sequence.
ACCESSION   DBNGSH010000645 DBNGSH010000000
VERSION     DBNGSH010000645.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 13403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13403
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4480929"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..299
                     /locus_tag="AC16CY_14840"
     CDS             45..299
                     /locus_tag="AC16CY_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR3355123.1"
                     /translation="MIETRGLVGMIEAADAMLKTANVVLVSWQKVDAGLVTALVRGDV
                     GSVKAATDAGAAAARRVGELVGVHVIARPADDLEKVFPIR"
     gene            complement(296..751)
                     /locus_tag="AC16CY_14845"
     CDS             complement(296..751)
                     /locus_tag="AC16CY_14845"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3355124.1"
                     /translation="MAIRLKKHYSSREVAAMTGLSARQLQWWDARRVFVSAIRSHRTE
                     AGGFTERRYTPLDVLELQVLADLRRRGFSVPQLRRLLTTLRDVFGVRLYEAIGDGGPM
                     ALFIGGDQLYARMVDGRLFNLDRPSEPLLMVGEELPLRPLTARQRRARR"
     gene            complement(755..1846)
                     /gene="thiO"
                     /locus_tag="AC16CY_14850"
     CDS             complement(755..1846)
                     /gene="thiO"
                     /locus_tag="AC16CY_14850"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HIR3355125.1"
                     /translation="MNITVVGAGVVGSAIAYELASRGADVTVLDARGPSLGATRASAG
                     ILAPHLEGHIPALLQLASCSLSMYDDFIRRVERDSGRRVEYERHGTLQVALSDREARQ
                     LSADALTLKQQGVDHVLLDPAGTQRVEPKLSDRATAGLLVPSHGYVCATSLTHALVAA
                     AEARGACVTTDTAIRIEGGQTSAVVTTKTHVVQADAVIVASGCWPIESRPSPAPPIRP
                     IRGQLVQLHLDEPVASRVIWGRDCYLVPWRDGSVLVGATSEDVGFDERSTADGIRGLL
                     NAAADLLPALGHARFQEVRVGLRPKGFDDLPIIGRSTTMPSVHYALGHYRNGVLLAPI
                     TAALVADLVIDGRERAELALVRPDRRAEG"
     gene            1932..3764
                     /locus_tag="AC16CY_14855"
     CDS             1932..3764
                     /locus_tag="AC16CY_14855"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3355126.1"
                     /translation="MTIPRPSGATLVTVALLILLPTLAVLQYQWVGQLSTAARERLQR
                     NVRIAAAQFREGFDVEILRAVLSLQVGPATVRESVWERYAERYTTWLNTAAHPQVVSN
                     VLVVDAEEGMLRLRRWNPDTDALEATEWRPVLSAWREQFEQELKEFQEGRPFDRRPSM
                     RGEGSLVIVPLRNLFIPTRRAPGSQTVTSVSVFGFTIIELNMPYIREQMLPELAARHF
                     THVEGDIYRVAVTTDGDPTSVLYRSDPDAPVDPRQADVSAALFVNRPQGFGLGRPPRP
                     GDGDGSLGPGPGPRLRSTDGPARRENEAVAQEPGRWRLLVQHQSGSLEAAVAHLRRRN
                     LGVSFGILLLLTVSIALLAATSRRAHRLAEQQMEFVAGVSHELRTPVAVIRSAAENLS
                     HGVVDGGDRVKRYGNLLETEAKRLGEMVERVLQYAGIEAGLTAGARVSLAPSEIIEGA
                     IESSLPMLEPERINVEREFAPNLPTVLGDGIALRSAVQNLIANAAKYGGRDRWIGIKT
                     EQAQHRRRSEVRITVSDHGPGIPADELPHIFDPFYRGADAVARQIHGNGLGLSLVRQI
                     VAAHGGRVTVTTRAGAGSSFTIALPAEPGQHPSAVTRELQPTHS"
     gene            3793..4479
                     /locus_tag="AC16CY_14860"
     CDS             3793..4479
                     /locus_tag="AC16CY_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3355127.1"
                     /translation="MSRLLLVEDEPGLVMTLTDRLVAEGYVVESEGDAVAGLAKATTG
                     TFDIILLDVMLPGGSGFDVCRTLRQRGIQTPILMLTARGQVVDRVVGLKLGADDYLVK
                     PFEMAELLARIEALLRRGAAASSTTVGDTYRFGDISIDLRKAEVTRAGQPIELSAREF
                     KLLRYFVEHRGAALSRDELLNEVWGYNAMPSTRTVDVHVAWLRQKIEDNPRHPQYIHT
                     VHGLGYKFVG"
     gene            4675..5568
                     /locus_tag="AC16CY_14865"
     CDS             4675..5568
                     /locus_tag="AC16CY_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/TrbI/VirB10 family type IV secretion system
                     protein"
                     /protein_id="HIR3355128.1"
                     /translation="MSRAATAGILAVVMAAAAGGTFLALRREAPPDPLKAPNAAAVER
                     PSAVTVTPSVTPTAPKAATRRPKPSAPPVKAPPETPAPSAQELPPGPEAVQNPEVVVP
                     VEPAIVADNDASRPVEPAGPVLVDLVVSADSVIGLQMESAVSSEKARVEDSVVAHVTR
                     DVRVGDRVVIPSGARVFGEVTVVERGGRMRDRARLGVRFTSVVLADGTRLPIRTETIF
                     REGDSPTPESAAKIGGGAVGGLIIGGILGGAKGAAIGGSIGAGAGTAAVMVGGRNHAS
                     LPAGSPLTVRLEEPVTVTLER"
     gene            complement(5755..8448)
                     /locus_tag="AC16CY_14870"
     CDS             complement(5755..8448)
                     /locus_tag="AC16CY_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3355129.1"
                     /translation="MSWHRFFRRASSDRDRAEELRSYLEIETDENIARGMPADAARRA
                     AHLKLGNTVRIREEIYRMNTIGPLETFGRDLRLALRGMRRRPGFTLAVVLTLALGIGA
                     NTAIFGVVDGVLIKPLPYPNAGELVSIKHLAPGLNVDELRMSPAQYFTYRDETRVFQQ
                     IGLYRDGGVTITGNGEPEQARLLGLTSDVLPALGVRPQLGRLFTEADAKGKGQQFPPV
                     VILTDAYWKRRFGGDPAVIGRRLIVDSDPVEVIGVMPEGFRFLDMEPAAEVIRLFPAD
                     RSRVAIGNFNFSSLARLKPGKTVADANADIARMLPIWLNAWPTAPNTPGRQEYEKWKI
                     APAVLPLKDDVVGGVADMLWILMATIGMVLLIACANVANLMLVRSESRRQEFAVRTAL
                     GARRGDIVRGVLVESLALSVIGGALGVALAYGGLAVVVATAPTSLPRVEDISLDPRAV
                     AFALVVSLISSMLFGALPALRQAAPHGAPIAGGARGASTSRERQRTRNALVVVQVALA
                     LVLLVGSGLMIRTSLALMRVPPGFTPGDAVQIARVAVPPLQVQDPERVTRLQHEILDK
                     IAALPGVTAAAFASAVPMEGPQRVWTQPVWVDGQRDTSVTPLRRIKTVSPGYFSAIGT
                     RLIAGRDITWRDIYGRAKVVLVSENFARELWGSPAAALGQRVRDSAPASDDGWREIVG
                     VVQDVHEDALYQAAPAFVYWPTMMENFGGNKVFTMRAVNLVIRSEGIGRESLLNGVRS
                     AVSSANSNLPVFLVRTMKDLYDQSMARTSFALVMLGIAAAMALALGVIGIYGVIAYVV
                     AQRSREIGIRLALGAAPTQLKRMFVRQGLALTAVGAGVGLVTAIALTQWMSSLLFGVG
                     RLDPATYTVVLGVLMMAAAMASYVPARRAAAVDPVETLTAE"
     gene            complement(8452..8802)
                     /locus_tag="AC16CY_14875"
     CDS             complement(8452..8802)
                     /locus_tag="AC16CY_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3355130.1"
                     /translation="MPKRESPMQSEMLQGTLDMLVLRTLVIGPAHGHTIAHAIERGSE
                     AVLQIEHGSLYPALHRLENRGWIVSFWGTSDNNRKAKYYRLTPTGRKQLAAQTTRWEE
                     IVRAIGRILNPSTE"
     gene            complement(8836..9741)
                     /locus_tag="AC16CY_14880"
     CDS             complement(8836..9741)
                     /locus_tag="AC16CY_14880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3355131.1"
                     /translation="MEWGLIVPGRESPLQSETPQGTLDMLVLRVLVIGPAHGHTIAYA
                     IERGSENVLQIEHGALYPCLHELENRGWIASFWGVVGDNRRAKYYRLTSAGRKQLFAQ
                     LTGREHASLVHLWLGVSIALAISAGPSAQMKDGRPTVEVTTDDRSSAGVELGIAGARV
                     NAIFITAGIRVVWRARTAVAIGTAGTDRIRLVVLSGPAADAMFLGHADLLGLAVPSAN
                     RVYIHYDRVLALARDYRTPPGWFLGIVIAHEVAHVLLPDAGHADDGLMAATLNPQATP
                     AFTAPQAQSLRAGLRGGVMSALGAR"
     gene            10190..10726
                     /locus_tag="AC16CY_14885"
     CDS             10190..10726
                     /locus_tag="AC16CY_14885"
                     /inference="COORDINATES: protein motif:HMM:NF047247.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HIR3355132.1"
                     /translation="MKLRALSILALVAFVALPTAAQVKKSDVAGVRNYSRVDATVGCG
                     GAVDPSAMSTLKKEGYVSVINLRQATEQGADIDGSRAAAKTAGLKYIHLPFNASSPDP
                     KVVSDFLGAVADKSNQPVFIHCGSANRVGAVWMIKRVLQDGWAIERAQTEAEAIGLQS
                     PQLVAFATSYIKEHGQKR"
     gene            complement(10723..11919)
                     /gene="metK"
                     /locus_tag="AC16CY_14890"
     CDS             complement(10723..11919)
                     /gene="metK"
                     /locus_tag="AC16CY_14890"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR3355133.1"
                     /translation="MSNRYIFSSESVGEGHPDKVADTISDAVLDACLAQDKFSRVACE
                     TYVKSNMVIIGGEITTKAKLDFVKIARDTIREIGYVNDDDVFHADRVFVNVLVTQQSP
                     DIAQGVDARKASGKKTAKQGAGDQGLMFGYASNETPELMPAPIMFAHRLGRELTRIRK
                     QGTVPWLRPDAKSQVSVVYENDKPVGIANVVISTQHAADVTHREIEKFCIEKVIKKVL
                     PARLLTSRTEFLINPTGRFVVGGPQGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPTK
                     VDRSAAYMGRWVAKTVVAAKLAARCEVQFAYAIGHPDPVSVHVDTFGTAAVPESKIVR
                     AINQVFAFQPAEIIEQLDLRRPIYTKTTNYGHFGKSDKDLTWESTSKVAALLKAIQTP
                     RLRAAG"
     gene            complement(12111..13211)
                     /locus_tag="AC16CY_14895"
     CDS             complement(12111..13211)
                     /locus_tag="AC16CY_14895"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355134.1"
                     /translation="MSRRLDVDITFRYRHGPVIEGAWSDSLDERGVTVLLGASGSGKT
                     TILRCLAGLEQPQRGHIRVGDRVWFDAGRRICLAPDRRDVGLLFQDYALFPHMTVERN
                     ITFGGARLDRASVRRRLEELVSIFQLDGLEKRRPRQISGGQQQRVALARAVFRRPEVL
                     LLDEPLSALDAAMREDLRNELRGLIRALDIPTYVVTHDRLDALALGDRTILMEGGRIV
                     QHGPTREVFGDPKTPTAARLVGVDTVLMGRIAAVAGGLATVRIGGQELRAEAPGGVGA
                     EAAFCIRAEDVMLARAVAGDMSAMNQWRGVIDYDTVEGPFVRVVVNCGFRLSALVTRD
                     AWQRLSLQSGDPVVAIVKAASIRVLLRGKGAP"
     gene            complement(13208..>13403)
                     /locus_tag="AC16CY_14900"
     CDS             complement(13208..>13403)
                     /locus_tag="AC16CY_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR3355135.1"
                     /translation="GEFGVVLMVGGNIPGVTRTIALAIYDDVQALDYAAANEASLLLL
                     VFAVAVLTLTYALQRRTVLL"
BASE COUNT         2292 a         4475 c         4481 g         2155 t
ORIGIN      
        1 cgcccgtggc acgggagaag gcggatagcg gcgacgcgct cggcatgatc gagacgcgcg
       61 gtctcgtcgg catgatcgaa gcggccgatg cgatgctgaa gaccgccaac gtcgtgctcg
      121 tgagctggca gaaggttgac gcgggcctgg tcacggcgct cgtgcgcggc gacgtgggct
      181 cggtcaaggc cgcgaccgat gcgggggcgg ccgccgcgcg ccgcgtcggc gagttggtcg
      241 gcgtgcacgt catcgccagg ccggccgacg atctggagaa ggtcttcccg attcgctaac
      301 gtcgcgcccg tctctgccgc gccgtcaacg gccgcaacgg gagctcctcc cccaccatca
      361 gcaacggctc gctcgggcga tccaggttga agagccgtcc atccaccatt cgggcgtaca
      421 gctggtcccc gccgatgaac agcgccatcg ggccgccgtc gccgatcgcc tcgtacagac
      481 ggacgccgaa cacgtcgcgc aacgtcgtca ggagccgccg cagctggggg acggagaaac
      541 cccggcgccg caggtctgcg agcacttgaa gctcgagcac gtccaacggc gtataccgcc
      601 gctcggtgaa acccccggct tctgttcgat gggaccgaat cgcggacacg aagacgcgac
      661 gggcgtccca ccactgaagc tggcgcgcgc tcagccccgt catcgccgca acttcgcgag
      721 agctgtagtg cttcttcagt cgtatcgcca cgtatcagcc ttccgcgcga cggtcgggcc
      781 tcacgagcgc cagctcggca cgctcccggc cgtcgatgac caggtcggcc acgagcgccg
      841 cggtgatcgg cgctaacaac actccgttcc ggtagtgacc cagcgcgtag tggacgctcg
      901 gcatcgtcgt ggatcggccg atgatgggca gatcgtcgaa ccccttcggc cgcagcccca
      961 cgcgaacttc ctggaagcgc gcatggccga gcgcaggaag aaggtcggcg gccgcgttca
     1021 gcagcccccg gattccgtcg gccgtggagc gctcgtcgaa accgacatcc tctgaggtcg
     1081 cgcccacgag gaccgacccg tcgcgccacg gcacgagata acagtcgcgg ccccagatga
     1141 cgcgcgaggc gaccggctcg tcgagatgaa gctgcaccag ctgcccgcga atcggccgga
     1201 tcggcggcgc gggcgacggc cgcgattcga tcggccagca gcccgacgcc acgatcacgg
     1261 catcggcctg gacgacgtga gtcttcgtcg tcacgaccgc ggacgtctgt ccaccctcga
     1321 ttcgaatcgc cgtgtcggtg gtgacgcatg caccgcgagc ctcggccgcg gcaacgagcg
     1381 catgcgtcag cgacgtcgca cacacgtagc cgtgcgaggg cacgagcaag ccggcggtcg
     1441 cgcggtcgct cagcttcggc tccacgcgct gtgtccccgc ggggtcgagc agcacgtggt
     1501 ccaccccctg ctgcttcagc gtcagagcat ccgccgagag ctgccgcgcc tcgcggtcgc
     1561 tcagcgctac ctgaagcgta ccgtggcgct cgtactcgac gcgcctgccg gagtcgcgct
     1621 cgacacggcg gatgaagtcg tcgtacatcg aaaggctgca cgaggccagc tggagcagcg
     1681 ccgggatgtg accttcgaga tggggcgcga gaataccggc tgaggcgcgc gtcgcaccga
     1741 gggaagggcc gcgagcgtcc agcaccgtga cgtccgcgcc gcgcgaggcc agctcgtagg
     1801 cgatcgcgga gcctacgacg cccgcgccga cgacggtgat gttcacgggg ggattctatc
     1861 tgtgactttg ttaagaatgt aaaagcttag aagtcaacgc cttacagcga ttcggtgcta
     1921 cactccgact ggtgacgatt ccgagaccat ccggcgcgac gctggtgact gtggcgctgc
     1981 tgattctgct gccgaccctg gcggtcctgc agtaccagtg ggttggacag ctcagtacag
     2041 cggcccgcga gcgcttgcag cgtaacgtgc gcatcgccgc tgcgcagttc cgcgaagggt
     2101 tcgacgtcga gatcctccgc gccgtcttga gcctgcaggt cggcccggca acggttcggg
     2161 agagcgtgtg ggaacggtac gccgagcgct acaccacctg gctcaacacg gccgcccatc
     2221 cgcaggtcgt ctccaacgtt ctcgtagtcg atgccgaaga agggatgctt cgtctgcgga
     2281 ggtggaatcc cgataccgat gcgctggaag ccacggagtg gcgtccggtc ctctccgcgt
     2341 ggcgggagca gttcgagcag gagttgaaag aattccagga ggggcggccg ttcgatcgca
     2401 ggccttccat gcgcggcgag gggtcgctcg tcatcgtgcc cctgcggaat ctcttcatcc
     2461 cgacgaggcg cgctccaggc tcgcagacgg ttacctccgt atcggtgttc ggcttcacga
     2521 tcatcgagct gaacatgccg tacatccggg agcagatgct gccagagctg gcggctcgtc
     2581 atttcacgca cgtcgaagga gacatctatc gcgtggccgt gaccaccgat ggcgatccga
     2641 ccagcgtgct gtacaggtcc gatcccgacg caccggtcga tccgcggcag gcggatgtgt
     2701 ccgcggcgct cttcgtcaac agaccccagg ggttcgggct cggccggccg cctcgccccg
     2761 gtgatggtga cggatctctg ggccccggcc cggggccgcg ccttcgaagc acggacggcc
     2821 ccgcccggcg cgagaacgag gccgtcgcac aagagccggg acgatggcgg ctgctcgtgc
     2881 agcatcagag cggatcgctc gaagccgccg ttgcgcacct gagacgacgg aatctcggcg
     2941 tcagcttcgg catcctgctg ctcctcacgg tcagcatcgc gctgctcgcg gccacgtcgc
     3001 gtcgcgctca ccggctggct gaacagcaga tggaattcgt ggcgggtgtg tcgcacgagt
     3061 tgcggacgcc ggtcgcggtg atccgatccg ccgcggagaa cctctcgcac ggcgtcgtgg
     3121 atggcggcga ccgcgtcaag cgctacggaa atcttctcga gaccgaagcg aagcgactcg
     3181 gcgagatggt cgagcgggtg ctgcaatacg caggcatcga ggcgggcctg accgcgggtg
     3241 ctcgcgtgtc gcttgccccg agcgaaatca tcgagggagc catcgaaagc tcgctcccga
     3301 tgctcgagcc ggaacggatc aacgtcgagc gcgagttcgc ccccaatctg ccgaccgtcc
     3361 tcggggacgg aatcgcgctg cggtccgcgg tgcagaacct cattgccaac gcggcgaagt
     3421 acggggggcg cgaccgatgg atcgggatca agaccgaaca ggcgcagcat cgccgtcgat
     3481 ccgaggtccg gattaccgtg agcgatcacg gcccgggaat tccggccgac gagctgccgc
     3541 acatcttcga tccgttttac cgcggtgccg acgccgtggc acggcagata cacggcaatg
     3601 ggcttggcct gtcgctcgtc aggcagatcg tcgcggccca cggtgggcgt gtgaccgtga
     3661 cgacacgggc cggcgccggc agttcgttca ccatcgcgct gccagcagaa ccgggccagc
     3721 atcccagcgc ggtcacccgc gagctgcagc ccacgcactc ctgaacgggg cccggccaag
     3781 ggaacttctc tcgtgtcgcg cttactcctg gtcgaagacg aacccggact cgtgatgacg
     3841 ctcacggacc gtctcgtcgc tgaaggctac gtggtcgaga gcgagggcga cgcggtcgcg
     3901 ggactggcca aggcgacgac gggtacgttc gacatcatcc tgctcgacgt catgctgccc
     3961 ggcggcagcg ggttcgacgt ctgccgcacg ctccgccagc gcggcatcca aactcccatc
     4021 ctcatgctca cggctcgcgg ccaggtggtc gatcgcgtcg tgggcctgaa gcttggcgcg
     4081 gatgactacc tcgtgaaacc gttcgagatg gcggagctgc tggcccgcat cgaggcgctg
     4141 ctgcggcgcg gcgccgcggc gtcgagcacc acggtgggcg acacgtaccg gttcggcgac
     4201 atctccatcg atctccgcaa agcggaagtc acgagagccg ggcagccgat cgagctgtcg
     4261 gcgcgcgagt tcaagctgct ccggtacttc gtcgagcatc gcggggccgc gttgtcgcgg
     4321 gatgagctgc tcaacgaggt gtggggctac aacgccatgc cttcgacgag gaccgtggac
     4381 gttcacgtcg cctggcttcg tcagaaaatc gaagacaatc cgcggcatcc ccagtacatt
     4441 cacaccgtcc acgggctcgg ctataagttt gtgggttgac tcagggggca gggggcaggg
     4501 ggcggcgggc aggggcaagg gcccagaccc ggacgacccc agagcctgaa ggaggacagg
     4561 tccggaaaac aggccagtgc cggtgggaac gggccgtcag atcaggcccg ttccgagccc
     4621 ctttcgaggc atgggccttg ctggagtcac ggttaagagg accccaaaat ggagatgagc
     4681 agggcggcga cggccggcat cctggcagtc gtcatggcgg cggccgccgg cgggacgttt
     4741 ctggccctgc gacgcgaggc tccgcctgat ccgctcaagg ccccgaacgc agccgcagtt
     4801 gagcggccgt cggccgtgac cgtgacgcct tctgtcacgc ccacagcgcc caaagcggcc
     4861 acgcgtcggc cgaagccgtc cgcgccgccg gtaaaggccc cgccggagac gccggcgccg
     4921 agcgcccagg aactgccacc cgggcccgag gccgttcaga accccgaggt cgttgtgcca
     4981 gttgagcccg cgatcgttgc cgacaacgac gcctcgcgcc ccgtcgagcc ggccggcccc
     5041 gtcctcgtgg atctcgtcgt ctcggccgac tcggtcatcg ggcttcaaat ggaatcggcc
     5101 gtctccagtg aaaaggcgcg ggtcgaagac agcgtcgtcg cgcacgtgac gcgcgacgtc
     5161 agggttggag accgcgtcgt cattccgtcg ggcgcgcgtg tgtttggcga ggtcacagtc
     5221 gtcgagcgag gcggcaggat gcgcgaccgc gccaggctgg gtgtgcggtt cacctccgtc
     5281 gtgctcgccg acgggacgcg gctccccatc cgcacggaga ccatcttcag ggagggcgac
     5341 tcacccactc ctgaaagcgc ggcaaagatc ggcggcgggg cggtcggcgg cctcatcatc
     5401 ggcgggattc tcgggggcgc caagggcgcc gccatcggcg gctcgatcgg cgcgggcgcc
     5461 ggcactgcgg cggtgatggt gggcgggcgg aaccacgcca gccttccagc gggatcgccg
     5521 ctgacggtgc gactcgagga gccggtgact gtcacgctcg agcgataagg cgactcgcaa
     5581 tccccaactc ccaattccca agtcccaatc gattctccac ttcgagattc cgactccaga
     5641 tgagaccgga ccagcgcgag ttcgtacccg ccggcgcctg tgcgcgcgca aggccgccac
     5701 tcagcagcgt cgtgagggcg aacgtgacca tcagtctctt gcgattcatg cgagtcactc
     5761 cgcggtcaac gtttcaacgg gatcgaccgc cgctgccctg cgcgccggca cgtagctcgc
     5821 catcgcggcg gccatcatca gcacaccgag cacgacggtg taggtggccg ggtcgagccg
     5881 gccgacaccg aacagcagcg acgacatcca ctgcgtcaac gcaatggctg tcaccaggcc
     5941 cactccggca ccaaccgcag tgagtgcgag cccctgacgt acgaacatcc gtttgagctg
     6001 cgtcggcgcg gcgccaagcg ccaggcggat gccgatctcc ctcgatcgct gtgcgacgac
     6061 gtacgcaatg acgccgtaga tgccgatcac gccgagcgcg agcgccatcg cggcggcgat
     6121 gcccagcatc accagcgcga acgacgttcg cgccatcgac tggtcataga gatccttcat
     6181 cgtccggacg aggaacacgg gcaggttcga gttcgcggac gacaccgcgc tcctgacgcc
     6241 gttcagcagg ctctctcggc cgatcccctc gctgcggatg accagattga cggcgcgcat
     6301 ggtgaagacc ttgttgccgc cgaagttctc catcatggtc ggccagtaca cgaatgctgg
     6361 tgcggcctga tacagcgcat cctcgtgcac gtcctggacg acgccgacga tctcgcgcca
     6421 gccatcgtcg ctcgcaggtg ccgagtcgcg aacgcgttga ccgagcgccg ccgccggaga
     6481 tccccagagc tcgcgagcga agttctcgga gacgagcacc accttcgcgc gtccgtagat
     6541 gtcgcgccac gtgatgtcgc ggccggcgat caggcgcgtg ccgatcgccg agaagtaccc
     6601 tggcgacacc gttttgattc ggcgcagcgg cgtcacggat gtgtcgcgct gtccatcgac
     6661 ccacaccggt tgcgtccaca cgcgctgcgg gccctccatc ggcacggcgc tggcaaacgc
     6721 agcggcggtg acgccgggga gcgcggcgat cttgtcgaga atctcgtgct gcagccgcgt
     6781 gacacgctct gggtcttgca cctgcagcgg cggcaccgcc actctggcga tctgcactgc
     6841 gtcgcctggc gtgaacccgg gaggcacgcg catgagagcg agagacgtcc ggatcatcag
     6901 gcccgagcca acaagcagga cgagtgcgag cgccacctgc acgacgacca gtgcattgcg
     6961 cgtgcgttgc cgttcgcggc tggtgctggc gccacgcgcg ccgccggcga tgggcgcgcc
     7021 atgcggcgcg gcctgcctca gcgcgggaag cgcgccgaac aacatgctcg atatcagtga
     7081 caccacgagg gcgaacgcga cggcacgcgg atccagcgag atgtcctcga cgcgcggcag
     7141 cgacgtgggc gcggtcgcga cgacgacggc gaggccgccg tacgcgagtg cgacacccag
     7201 cgcgccacca atgactgaca gcgccaggct ttcgaccagc acccctcgca cgatgtcgcc
     7261 tcgtcgtgca ccgagcgccg tccgtaccgc aaactcctga cggcggctct cggatctcac
     7321 cagcatgagg ttcgcgacgt tggcgcaggc gatcagcagc accatgccga tggtggccat
     7381 gaggatccac agcatgtcgg cgacgccgcc gacaacgtcg tccttcagcg gcagcacggc
     7441 cggcgcgatc ttccacttct cgtactcctg ccgtcccgga gtgttcggcg ctgtcggcca
     7501 cgcgttgagc cagatcggca acatccgtgc gatgtcggcg ttggcgtccg cgacggtctt
     7561 acctggcttg agccgggcga ggctcgaaaa gttgaagttc ccgatggcca cgcggctgcg
     7621 atcggccggg aacaaccgaa tcacctcggc ggcgggctcc atatcgagaa accggaatcc
     7681 ctcgggcatg acgccgatga cctcgacggg atcggagtcc acaatcagtc gtcgtccgat
     7741 cactgcgggg tcgccgccaa accggcgttt ccagtaggcg tcggtcagga tgacgaccgg
     7801 agggaactgc tgtcccttgc ctttcgcatc ggcctccgtg aagagccggc ccagctgcgg
     7861 ccgaacaccc agtgccggca ggacgtcgga ggtgagcccc agcagacgcg cctgttccgg
     7921 ctcgccgttt ccggtgatcg tgaccccgcc gtcacggtac agaccaatct gttgaaagac
     7981 gcgcgtctca tcgcggtacg tgaagtactg cgccggcgac atgcgcagct catcgacgtt
     8041 gagacctggc gcgaggtgct tgatgctgac cagttcgccc gcgttcgggt acggaagcgg
     8101 cttgatcagc acgccatcga cgacgccgaa gatggccgtg ttcgcgccaa tgcccaacgc
     8161 gagcgtgagc acaacggcca gcgtgaaccc tggacgccgg cgcatgccgc gtaatgccag
     8221 gcgcagatcg cggccgaacg tctcgagcgg gccgatcgtg ttcatccggt agatctcttc
     8281 gcggatccgg accgtgttcc cgagcttgag gtgcgccgcg cggcgcgccg catcggccgg
     8341 catcccgcgc gcgatgttct cgtcggtttc gatctcgaga taggaacgca gctcctcggc
     8401 gcgatcgcga tccgaggacg ctcgacggaa gaagcggtgc cagctcatgg cttactccgt
     8461 tgacggattg aggatcctgc cgatggcgcg aacgatctct tcccaccgcg tggtctgcgc
     8521 ggcgagttgt ttgcggccgg tcggggtcaa ccggtagtac ttcgccttgc ggttgttgtc
     8581 ggaggtgccc cagaacgaca cgatccagcc gcgattctcc agccggtgaa gggccgggta
     8641 gagagacccg tgctcgatct gcagcacggc ttctgacccg cgctcgatgg cgtgcgcaat
     8701 ggtgtgaccg tgcgcaggtc caatgaccag ggtcctcagg accagcatgt ccaatgttcc
     8761 ctggagcatt tcactctgca tcggcgattc gcgtttcggc atcgttctcc tcgtgttgat
     8821 cggcgcggac gaacgtcagc gagcgccgag ggcggacatc accccgccac gcaggcctgc
     8881 gcgaagtgac tgcgcttgtg gagcggtaaa agccggcgtg gcctgaggat tgagcgtggc
     8941 agccatcagc ccgtcgtcgg cgtgcccggc atcgggaagc agcacgtgcg ccacttcgtg
     9001 tgcgatcacg atgccgagga accatcccgg cggcgtcctg tagtcgcgcg cgagagccag
     9061 tacccggtcg taatggatgt agacccggtt cgccgacggc actgccaggc cgagcaggtc
     9121 ggcgtgaccg agaaacatcg cgtccgctgc gggcccgctg agtactacga gccggatgcg
     9181 atccgttccg gccgtaccga tggcaacagc cgttcgcgca cgccagacaa cccggatgcc
     9241 cgccgtgatg aagatcgcgt tgactcgcgc gccagcgatc cccagttcaa cgccggccga
     9301 cgatcggtcg tcggtcgtca cttcgacggt cggtcgtcca tccttcatct gagcgctcgg
     9361 ccctgcagag atggcaagcg cgatcgagac tccgagccag aggtgtacga gtgaggcgtg
     9421 ctcccgtccg gtgagctggg cgaagagttg cttccgcccc gcagacgtga ggcggtagta
     9481 cttggctcga cggttgtcgc cgacgacacc ccagaaagac gcgatccagc cgcgattctc
     9541 cagctcgtga aggcagggat agagggcgcc gtgctcgatc tgcagcacgt tctccgatcc
     9601 tcgctcgatg gcgtacgcga tcgtgtgacc atgcgccggc ccgatcacga ggacccgcag
     9661 tacgagcatg tcgagcgtgc cctggggcgt ctcgctttgg agcggcgatt cacgccccgg
     9721 cacgatcagc ccccattcca cacggtgatc tatgcatcag aatcccttca aaattgccca
     9781 tacgagagga tctttacact ttgctcagaa gattatctag tggaatatgg gcgcccccag
     9841 ctgacgagga gctagacctg ttgggtgggc cactcaggca ggaaccgagg gcagcgatgg
     9901 attacccgcg ggaagtcgcc cagtgttgac ctatcaggga ccgaaggctg tcggattttc
     9961 cctcctcatg gcgaagagac gcctgaacaa gttttccggg cccgcgcccg tatttggatc
    10021 catgccccac ggatcaagtc gcattcttca ggcagtcacg cacaaagtcg gtgcaattcc
    10081 gaccgaacag agatcaccgt cgagaggtcg gacgccacgt ggggtcgcgc agggccggct
    10141 ctgccggccc tttccacgta cggatgtaag attcgcgtgg aggcgagcta tgaaacttcg
    10201 ggctctttcg atactggcgc tagtcgcgtt cgtggcgctg ccaacggccg cgcaggtcaa
    10261 gaagtcggac gtcgccggcg tccgcaacta ctcacgcgtg gatgcaaccg tgggatgcgg
    10321 gggcgctgtt gatccgagcg cgatgtccac gctcaagaag gaaggctacg tgtcggtgat
    10381 caacctgcgg caggcaaccg agcagggcgc cgacattgac ggaagccggg cggccgccaa
    10441 gacggccgga ctcaagtaca tccacctccc cttcaacgcc tcgtcaccgg atccgaaggt
    10501 ggtgtccgat ttcctggggg ccgtcgccga caagagcaac cagccagtct tcatccactg
    10561 cggctcggcc aaccgcgtgg gcgccgtatg gatgatcaag cgggtgctgc aggacggctg
    10621 ggcgatcgag agggcgcaga ccgaggcgga agccatcggc ctgcagagcc ctcaactcgt
    10681 cgcgttcgcg acgagctaca tcaaggaaca cgggcagaag cgctaacccg cggctcgcag
    10741 cctcggggtc tggatcgcct tcaacagggc ggcgaccttg ctggtcgact cccacgtgag
    10801 gtccttgtcg ctcttgccga agtgtccgta gttggtcgtc ttcgtgtaga tcggacgcct
    10861 gaggtccagc tgctcgatga tttccgccgg ctggaacgcg aacacctggt tgatggcgcg
    10921 gacgatcttc gactccggca cggcggcggt gccgaaggtg tccacgtgga cgctcacggg
    10981 atcggggtgg ccgatcgcgt aggcgaactg tacctcgcac ctggcggcca gcttcgcagc
    11041 cacgacggtt ttggcgaccc agcggcccat gtacgccgcg ctgcggtcca ccttcgtcgg
    11101 atccttgccc gagaacgcgc cgccgccatg acgtcccatg ccgccgtagg tatcgacgat
    11161 gatcttgcgt ccggtcagtc ccgtgtctcc ctgcggcccg ccgacgacga accggccggt
    11221 ggggttgatc aggaactcgg tcctgctggt gagcagcctc gccggcagca ccttcttgat
    11281 caccttctcg atgcagaact tctcgatctc acggtgggtg acgtcggccg cgtgctgcgt
    11341 cgagatgacg acgttggcga tgccgacggg cttgtcgttc tcgtagacga ccgacacctg
    11401 cgacttggcg tcgggccgca gccacggcac ggtgccctgc ttgcggatcc gggtcagctc
    11461 gcgcccgagg cggtgcgcga acatgatcgg cgccggcatg agctcgggcg tctcgttgct
    11521 ggcgtagccg aacatcaggc cctggtcgcc ggcaccctgc ttcgcggtct tcttgccgct
    11581 cgccttgcgc gcgtcaacgc cctgggcgat gtccggggac tgctgcgtca caagcacgtt
    11641 cacgaacacg cggtcggcat ggaagacgtc gtcgtcgttg acgtagccga tctcgcgaat
    11701 cgtgtcgcgc gcgatcttga cgaagtcgag cttggccttg gtggtgatct cgccgccgat
    11761 gatcaccatg ttcgacttca cgtaggtttc acaggcgacg cggctgaatt tgtcctgggc
    11821 caggcacgca tcgaggacgg catcggagat ggtgtcggcg accttgtcgg ggtgcccttc
    11881 gccgacggat tccgacgaga aaatgtatcg gttgctcata acactccaca aaaaaagccc
    11941 gcgaggtcgc gggctgctcg cgacttagac agatcggccg gaacttccgg cgcgcgcggc
    12001 ccatcaagtc ggtgaaatcg ccgcacgaac gaccctcaag tatcgttcgc cgtacctgac
    12061 agtgtcaacc gcgcgcactc gatcaggggg caggggtcag ggggtagggg tcagggggcc
    12121 cccttccccc tcaacagcac ccggatgctg gccgccttga cgatggccac gacgggatcg
    12181 ccggactgga gcgagagccg ctgccacgcg tccctggtga ccagcgcgct cagccggaag
    12241 ccgcagttca ccaccacgcg cacgaagggg ccttcgaccg tgtcgtagtc gatcacccct
    12301 cgccactgat tcatcgcgct catgtcaccg gcaacggcac gggcgagcat gacgtcctcg
    12361 gcgcggatgc agaaggccgc ctcggcgccg acgccgcccg gcgcttccgc cctcagttcc
    12421 tggccgccga tccggaccgt cgcgagcccg ccggccaccg ccgcgatccg ccccatcagc
    12481 accgtgtcca cgccgacgag ccgggctgcg gttggcgttt tcggatcccc gaagacctcg
    12541 cgcgtcggcc cgtgctgaac gatgcggccg ccttccatga ggatcgtccg atcgccgagc
    12601 gcgagcgcgt cgaggcgatc gtgggtcacg acgtaggtgg ggatgtcgag cgcgcggatg
    12661 agaccgcgca gctcgttcct cagatcctca cgcatcgcag cgtcgagcgc cgacagcggc
    12721 tcgtcgagca ggagcacttc cggacggcgg aagacggcgc gagccagcgc gacgcgctgc
    12781 tgctgaccgc ccgagatctg acgcggccgc cgtttctcta ggccgtcgag ctggaagatc
    12841 gagaccagct cctcgagacg gcgcctgacg gacgcgcggt cgaggcgagc gcctccgaag
    12901 gtgatattcc gttccacggt catgtggggg aagagcgcgt aatcctggaa caggagcccg
    12961 acgtcgcggc ggtcgggagc caggcagatc cggcgcccgg cgtcgaacca cacgcgatcg
    13021 cccacgcgaa tgtgtccgcg ctgaggctgc tcgagtcccg ccaggcaccg gagaatcgtc
    13081 gtctttccgg atcccgatgc tccgaggagc accgtgacgc ctcgctcgtc gagcgagtcg
    13141 gaccaggcgc cctcgatgac cggtccgtga cgatatcgaa aggtgatgtc gacgtcgagc
    13201 cgccggctca cagcagtacc gttcgccgct gcaacgcgta ggtcagcgtg agcaccgcca
    13261 ccgcgaagac gagcagcagc agcgatgcct cgtttgccgc tgcgtagtcg agcgcctgca
    13321 cgtcgtcgta gatggcaagc gcgatcgtgc gcgtgacgcc ggggatgttg ccgccgacca
    13381 tcagcacgac gccgaactcc ccc
//
LOCUS       DBNGSH010000646         8356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1594833, whole genome shotgun sequence.
ACCESSION   DBNGSH010000646 DBNGSH010000000
VERSION     DBNGSH010000646.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1594833"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="AC16CY_14905"
     CDS             complement(<1..300)
                     /locus_tag="AC16CY_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanethiol S-methyltransferase"
                     /protein_id="HIR3355136.1"
                     /translation="MSRILVFAYGLIAYALFGVTYLYAVGFVGNLVVPKSLDSMPTAP
                     FATALLINLGLLSLFAVQHSVMARPAFKRLLLRVIPAAAERSTYVLASSLALLLLF"
     gene            543..2345
                     /gene="lepA"
                     /locus_tag="AC16CY_14910"
     CDS             543..2345
                     /gene="lepA"
                     /locus_tag="AC16CY_14910"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR3355137.1"
                     /translation="MDQRYIRNFSIIAHIDHGKSTLADRFLEITGALQAREMEAQVLD
                     AMDLERERGITIKAHPVRLNYTASDGQQHVLNLIDTPGHVDFSYEVTRSLAACEGALL
                     LVDASQGVEAQTLANAYLAVDNNLEIIPVINKIDLPSAQPDECKRQLEEIIGLDASGA
                     ILASAKEGTGTREILEAIVARVPPPTGDPAAPLKALIFDSWYDAYRGVVIVVRVIDGV
                     LRAKQKVRLMAMGQEYEVDELGVFSPKATPVDELSVGDVGFIVANIKRVSDAKIGDTV
                     TESARPTPEPFPGFKELKPMVFAGLYPVEGHKYTELREALEKLRLNDASFFYEPETSA
                     ALGFGFRCGFLGLLHMEIVQERLEREYDMDLVTTAPGVLYRVTTTDGEVQEVDSPAKL
                     PEAGRISRLEEPVITAMILTPAEHVGAILQLCQDKRGIQKKLEYLKSDRVLITYDLPF
                     NEVVLDFYDKLKTLSRGYASLDYHVTGYWESPLVKMDILVNGEPVDALSIIVHRDAAY
                     ERGRALASKMRELIPRQMFEVAIQASIGNRIIARESVKALRKNVLAKCYGGDITRKRK
                     LLEKQKEGKKRMKRVGRVEIPQEAFLAVLKVGTD"
     gene            2588..3277
                     /gene="lepB"
                     /locus_tag="AC16CY_14915"
     CDS             2588..3277
                     /gene="lepB"
                     /locus_tag="AC16CY_14915"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3355138.1"
                     /translation="MQKVETTAKGEAATEPSFRKSTAREYFESICVAVVLALFVRTFV
                     VQAFKIPTGSMENNLLIGDHLLVNKFVFAPTLTGAEDALLPIDPIRRGDILVFKYPKD
                     PERDFIKRVIGLPGETIELRDRKVHINGTPIDEPYVQFLFPFDSTETEPGVPNPRRSY
                     GPVTVPPGYYFMMGDNRDNSEDSRYWGFMPRDYVKGKALFVYFSFGDGSTGLSGALGN
                     VRWGRILHQIH"
     gene            3285..3644
                     /locus_tag="AC16CY_14920"
     CDS             3285..3644
                     /locus_tag="AC16CY_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355139.1"
                     /translation="MKTVLKLVIAVAFLNAVIRGADSMWSYYKLKDEAQRTLLFGSTS
                     TTEELQSQILLAAAELEIPLTAERLIVTRDGRRSVAEGSYTQQVEFFPNYPYPVEFSF
                     VVDSVAISPAVPEPRKR"
     gene            3732..3977
                     /locus_tag="AC16CY_14925"
     CDS             3732..3977
                     /locus_tag="AC16CY_14925"
                     /inference="COORDINATES: protein motif:HMM:NF021253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HIR3355140.1"
                     /translation="MTSRAQDVLREALALPLEERADVAAELLASLDDAAADRPADVEA
                     AWAAEVERRARRAMADESGGVPWDDVRRRAEAELRKR"
     gene            3974..4288
                     /locus_tag="AC16CY_14930"
     CDS             3974..4288
                     /locus_tag="AC16CY_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3355141.1"
                     /translation="MNPHVRFDDEADAEYRFAGRWYEARRAHLGIEFFDAVDATINQI
                     VAMPRAGSRVRGLPADLIVRRQAVTRFPYHVIYLEMAAHIRILAIAHDRRKPGYWRDR
                     LK"
     gene            complement(4408..4929)
                     /locus_tag="AC16CY_14935"
     CDS             complement(4408..4929)
                     /locus_tag="AC16CY_14935"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3355142.1"
                     /translation="MGNRTPAITKRRNRAPIPYDVQVRVLFRDGWICRWCRRPVVFGP
                     ALRLLQEFAAGSGYSRPLAYFHKNWSRAGAPLLDHMGAVIDHVEAFAKGGVHEEANFV
                     VACNKCNTRKNDHHAADYERRNPPRPVRGKYGEPEHWDGLASMFLVLAARGATLGPSE
                     ARWEKALRKHLAL"
     gene            complement(4974..5831)
                     /locus_tag="AC16CY_14940"
     CDS             complement(4974..5831)
                     /locus_tag="AC16CY_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR3355143.1"
                     /translation="MPDDQWTRMLGFNSLLTAAGLDPAKVRLVRHRHGREYQRLVYQD
                     AIRQNPRFEQYQSGQQNPTVIDQMCSADSIASFVVDPGGETVFVGLWRVKGSRKAYVP
                     DPYITVARPPLAGSVTIELERMAALREYCGRIVIDWGGGERAWVQYANRRDKEIIEVR
                     RHAEEPHFPGFGRFSCGLHEIDALPATWLEALRASRGVYLLVHRETGAQYVGSATGDD
                     GFIGRWLGYADGHGGNAAMRELAHSPEQYDVRILETVGSGATPDEVCDLESLWKEKLG
                     SRAKGLNRN"
     gene            complement(5864..6532)
                     /locus_tag="AC16CY_14945"
     CDS             complement(5864..6532)
                     /locus_tag="AC16CY_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355144.1"
                     /translation="MADHTASDTLPSGVEGLQAANADLRVEVDRCRAENERLEAENVD
                     LHDEVEELQARVDADLEAEIDELRAENERLEAENAELDDRVDGLEARVDTDLEGEIDE
                     LRAENERLEAENAELDDRVDELEARVDTDLEAEIDDLRAENERLEAEKTDAPGRSFRK
                     AWETEGRPSDEEPVYVASKNRNKFHRPSCKWAEYIGPSTLLEFGSHREAVEAGYKPCG
                     TCRA"
     gene            complement(6652..7389)
                     /locus_tag="AC16CY_14950"
     CDS             complement(6652..7389)
                     /locus_tag="AC16CY_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3355145.1"
                     /translation="MTERINARMSAVGGVFDMSSATPRCIGSSSAFGSATTFVTAAHI
                     VAGRRLDEIAVNHFGHSRHQFSPINEIVVDQHLDAALLDVVVEEPRWILPFTHALGAA
                     WYGSEVASIGWPVDLLAPDADRETLRCFRGYIQRTFLYDPPGRGSYPAFELSFAAPPG
                     LSGSPVFLVDEPTTLVGIVTANFSTHSLIEATEETADTTRAIQRTEYRSVITYGIATI
                     AWHVWHAFEDRLPAVALASARQDQRTG"
     gene            complement(7980..>8356)
                     /locus_tag="AC16CY_14955"
     CDS             complement(7980..>8356)
                     /locus_tag="AC16CY_14955"
                     /inference="COORDINATES: protein motif:HMM:NF016825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR3355146.1"
                     /translation="MSALRLPKADETQAAARRHALDAAMRSATEAPLETMRLCRRALG
                     EAPAVAERCVPSAASDVGVAIELLRAAVHGAEMNVDTNLASVGDAGYVSGVSAEKQQL
                     AKESHADADRGLALVARARAPR"
BASE COUNT         1543 a         2652 c         2619 g         1542 t
ORIGIN      
        1 gaacagcaga agcagcgcga ggctgctggc cagcacatag gtgcttcgct cggccgctgc
       61 cggaatcaca cgaagcagga ggcgcttgaa cgccggccgc gccatcacgc tgtgctggac
      121 ggcgaaaagg ctcagcaggc ccaggttgat caggagcgcc gttgcgaagg gcgccgtcgg
      181 catcgagtcc agggacttgg gcacgacgag attcccgacg aatccgacgg catagagata
      241 ggtcacaccg aacagggcgt aggcaatcag cccgtacgcg aacacgagaa ttctgctcat
      301 cactgcctcc attcgttagc agccggtcgg cggccggccg ccgcaggcaa ggcaacggct
      361 gggcccgccg cgcggcgccg ctaacgtttg cgttgtcacg atgttgaaga cgagcgaggc
      421 tgacggagga gctgtcgaac tgtcgaatat tgggcagttc gacagcggga aggtacaatt
      481 cagcgttcga agctcgttgt tcgcggtact cttccagttg atacggccca ctagtctcgc
      541 tgatggatca acgttacatc cgcaattttt cgatcatcgc gcacatcgac cacggcaagt
      601 cgacgctggc ggatcgcttc ctggagatca ccggcgcgct tcaggcgcgc gagatggagg
      661 cgcaggttct cgacgcgatg gacctcgagc gtgagcgcgg catcacgatc aaggcccatc
      721 cggtccgctt gaactacacc gcgagcgacg gccagcagca cgtgctgaac ctgatcgaca
      781 ccccaggcca cgtcgatttc agctacgagg tgacacggtc gctggcggcg tgcgaagggg
      841 cgctgctgct ggtcgatgcc tcgcagggcg tcgaggcaca gacgctcgcc aacgcctacc
      901 tcgcggtcga caacaacctc gagatcatcc ccgtcatcaa caagatcgac ctgccgagcg
      961 cacagccgga cgaatgcaag cgccagctcg aggagatcat cggcctcgac gcctcgggcg
     1021 cgatcctggc gagcgcgaag gagggcaccg gcactcgcga gattctcgag gcgatcgtgg
     1081 cgcgcgtccc tccgcccacc ggcgatccgg cggcgccgct caaggcgctg atcttcgatt
     1141 cctggtacga cgcctaccgc ggcgtggtca tcgtcgtacg cgtgatcgac ggcgtgctca
     1201 gggcgaagca aaaggtccgc ctgatggcga tggggcagga gtacgaggtg gacgagctgg
     1261 gcgtgttctc gccgaaggcc acgccggtcg acgagctgag cgtcggcgat gtcggcttca
     1321 tcgtggccaa catcaagcgc gtctccgacg ccaagatcgg cgacacggtc accgaatcgg
     1381 ccaggcccac cccggagccc tttcccggct tcaaggagct gaagccgatg gtcttcgccg
     1441 ggctgtaccc ggtggagggc cacaagtaca ccgagcttcg agaggcgctc gagaagctga
     1501 ggctgaacga cgcgtcgttt ttctacgagc cggaaacgtc ggcggcgctg ggcttcggct
     1561 tccggtgcgg ctttctcggg ctgctccaca tggagatcgt ccaggaacgc ctcgagcgcg
     1621 agtacgacat ggacctggtg acgacggcgc cgggcgtgct gtatcgcgtg acgacgaccg
     1681 acggcgaggt gcaagaggta gacagcccgg cgaagctgcc ggaggcgggc cggatatcca
     1741 ggctcgagga gccggtcatc acggccatga tcctgacgcc ggccgagcat gtcggcgcca
     1801 ttctccagct ctgccaggac aagcggggca tacagaagaa gctcgagtac ctgaagtcgg
     1861 accgcgtgct catcacctac gatctgccgt tcaacgaggt ggtgctcgac ttctacgaca
     1921 agctgaagac gctctcacgc ggctacgcgt cgctggacta ccacgtgacg ggatactggg
     1981 aatcgccgct cgtgaagatg gacatcctcg tgaacggcga gccggtggac gcgctctcga
     2041 tcatcgtcca tcgcgacgcc gcgtacgagc gcggccgcgc gctggcgtcg aagatgcgcg
     2101 agctcatccc gcggcagatg ttcgaggtcg cgatccaggc ctcgatcggc aatcgcatca
     2161 tcgcgcggga atcggtgaag gcgctgcgca agaacgtgct ggccaagtgc tacggcggcg
     2221 acatcacgcg caagcggaag ctgctcgaga agcagaaaga gggcaagaag cgcatgaagc
     2281 gggttggccg ggtggagatc ccgcaggagg cgttcctcgc agtactcaag gtcggaaccg
     2341 actagtggaa cgtatctcct ggattactag cggccgctcc gttgtcgcgg ccgcgaaagt
     2401 gcggtcgaaa ggctgtcgtc ggtgctacgg gttcagttga tacagtccac taaccccgga
     2461 actcgcttgt gtcggtgatg ttgccaacga tcgtggcttg cagggcaggc tgtttggtca
     2521 gcggggcctc ggccggagcc caccaacaca agcgagctgt ggtgtagtgc agaatgcaca
     2581 aggcaacatg caaaaggtag agacaacggc gaagggcgag gcggcgacag agccgtcgtt
     2641 ccggaagtcc accgcgaggg agtacttcga gtcgatctgc gtggccgtgg tgctggcgct
     2701 gttcgtgcgc accttcgtcg tccaggcctt caagattccg accggatcga tggaaaacaa
     2761 cctgctcatc ggcgatcatc tgctcgtgaa caagttcgtc ttcgcgccga cgctgacggg
     2821 cgccgaagac gcgctgctgc cgatcgatcc catccgccgg ggcgacatcc tcgtgttcaa
     2881 gtatcccaag gatcccgagc gcgatttcat caagcgcgtc atcgggctcc caggcgagac
     2941 gatcgagctc cgcgacagga aggtgcacat caacgggacc ccgatcgacg agccgtacgt
     3001 gcagttcctc tttcccttcg actccacgga gaccgagccg ggagtgccca atccgcggcg
     3061 cagttacggc ccggtgacgg ttccgccggg ctactacttc atgatgggcg acaatcggga
     3121 caactcggag gacagccgct actggggatt catgccgcgg gactacgtga aaggcaaggc
     3181 gctcttcgtg tatttttcgt tcggcgacgg ctcgacgggg ctgtcgggcg cgctcggcaa
     3241 cgtgcggtgg ggcaggatcc tgcaccagat acactgaagc gctcatgaag accgtcctca
     3301 aactcgtgat cgcggtggcg ttcctcaacg ccgtgatccg gggtgcggac tccatgtgga
     3361 gctactacaa gctcaaggac gaggcgcagc ggacgctgct cttcggctcc acgtccacga
     3421 cagaggagct gcagtcacag atcctcttgg cggccgccga gctggaaatc ccgctgaccg
     3481 ctgaacgcct catcgtgaca cgagacgggc ggcgcagcgt tgccgagggc tcgtacacgc
     3541 agcaggtcga gttctttccc aactacccgt acccggtcga attctcgttc gtcgtggact
     3601 cggtcgccat cagcccggcg gttcccgaac cgcgcaagcg gtagcactca gcgccgggtc
     3661 ttcgcggcag caacaagcgc gagaccacga ggtcggccat gttgggcagt tggtccgtgt
     3721 agaatggccg catgacgtca cgagcgcaag atgtcctgcg cgaagccctg gcgctgcccc
     3781 tcgaggaacg tgctgacgtc gcggccgaac tgttggccag tcttgacgac gcggcggcgg
     3841 accgccccgc cgatgtcgaa gcggcctggg cggccgaggt cgaacgacga gcccgccggg
     3901 ccatggccga cgagtccggc ggagtgccgt gggacgacgt gcgccgtcgt gctgaggccg
     3961 aactccgcaa gcggtgaatc ctcacgttcg ctttgacgac gaggcggacg ccgaataccg
     4021 cttcgccggc cgatggtacg aggctcggcg agcgcacctg ggcatcgagt tcttcgacgc
     4081 cgttgacgcc actatcaacc agatcgttgc gatgcctcgc gccggttccc gcgtccgggg
     4141 cctgccggct gacctcatcg ttcgacgcca agccgtgacg cgctttccct atcacgtgat
     4201 ctacctggaa atggcagcgc acatccgcat cctcgccatt gcgcacgacc gccggaagcc
     4261 gggttactgg agagaccgcc tgaagtagca tcgatcggtg ccgcttcacc ttcggcgttc
     4321 gttagtccgc ccaacgtgcc gcttcagccg cggccgctca ggatcgcacc ggccaccgtc
     4381 ggctgcaagc ggatgcgcgg tggcccttca gagtgcgaga tgtttgcgta atgccttctc
     4441 ccaacgagcc tccgaagggc caagtgttgc gccccgggct gcaagcacaa ggaacatcga
     4501 agcgagacca tcccaatgtt ctggttcccc gtacttgccg cgaaccggtc tcggaggatt
     4561 cctgcgttcg tagtcggcag cgtgatggtc attcttccgc gtattgcact tgttgcatgc
     4621 cacaacgaag tttgcctctt cgtgaacccc gcctttcgca aatgcctcga catgatcaat
     4681 gacggcaccc atatggtcga gcaagggcgc accagcacgg ctccagtttt tgtgaaagta
     4741 ggcgagcggc cttgagtatc cggatccagc tgcgaattcc tgcagcaaac gcaaagcggg
     4801 cccaaacact acgggccggc gacaccagcg acagatccag ccgtctcgaa aaagcactcg
     4861 tacttgaacg tcatatggaa ttggagctcg attccgccgc ttcgtgattg ctggagttct
     4921 gttgcccatc gcttcagatc aagtccacct aaacttgcgc tcggcggcgt gtctcagttt
     4981 cgattaagac ccttggcccg cgagccgagt ttctctttcc acaatgattc gaggtcgcag
     5041 acctcatcgg gtgtcgcgcc ggagccgacg gtttcgagaa tgcggacatc gtattgttcg
     5101 gggctgtggg caagttctcg catcgccgcg ttgccaccgt gcccgtcggc gtacccaagc
     5161 cagcgaccga tgaagccatc gtctccggtg gccgacccga cgtattgcgc tccggtctca
     5221 cggtgaacaa gcagatagac cccgcggctc gcacgcaacg cttcgagcca ggtcgccggc
     5281 agagcatcga tttcgtggag gccacaactg aatcggccaa agccaggaaa gtgcggctcc
     5341 tcggcgtgtc ggcggacttc gatgatctcc ttgtcccgtc gattcgcgta ctggacccag
     5401 gcacgctcgc cgccacccca atcgatgaca atccggccgc agtactcccg caaagcagcc
     5461 attcgttcca actcgatcgt gacactcccg gccaggggcg gcctcgccac agtgatgtac
     5521 ggatctggaa cgtacgcttt gcgcgaaccc tttacacgcc agagtcctac aaacacggtc
     5581 tcgccgccag gatcaacgac gaatgacgcg atcgagtcgg cggagcacat ctggtcaatg
     5641 accgttgggt tttgttggcc ggattgatat tgctcaaacc tcggattctg gcggatggcg
     5701 tcctgataca cgagtcgctg gtactcgcgc ccatgacgat ggcgcaccaa tcgaaccttg
     5761 gcgggatcga ggccggcggc tgtgaggagg ctattgaatc cgagcatcct tgtccattga
     5821 tcgtcgggca cgatcggaat tcgtccgccg cggttaggcc gattcaagcc cggcacgtcc
     5881 cgcaaggttt gtatccagcc tccacagctt cacggtgcga tccaaactcg agcagcgtac
     5941 ttggcccgat gtactcagcc catttacaac tgggcctgtg gaacttgttt cgattcttgc
     6001 tggcaacgta gacaggttcc tcgtcgcttg gccttccttc tgtttcccat gccttacgga
     6061 atgacctgcc aggcgcgtcg gtcttctcag cctcgagccg ttcgttttcc gctctgaggt
     6121 cgtcgatctc ggcttcgagg tccgtgtcga ctcgcgcctc cagttcgtcg actcggtcat
     6181 caagttcggc attctccgcc tccaaccgct cgttctcggc tcggagttcg tcgatctcgc
     6241 cctcgagatc cgtatcgact cgtgcctcca agccgtcgac ccggtcgtca agttcggcgt
     6301 tctccgcctc caaccgctcg ttctcggctc ggagttcgtc gatctcggct tcgaggtccg
     6361 cgtcgacgcg cgcctgcagt tcttcaactt cgtcgtggag gtcgacgttt tccgcttcca
     6421 gccgctcgtt ttctgcgcga catcggtcga cctccacgcg gagatcggcg ttcgctgcct
     6481 gaaggccctc tacgccggaa ggaagggtgt cactagccgt atggtcagcc accgttgcga
     6541 ccctcagcca ttgaagcaga cccaggattc gtcaatgctg cgttccaatt cggcctaacg
     6601 gaaacgcatc accagcgggg cttcccgatt ctccgccggg tgcatgcggt gttagccagt
     6661 ccgttggtcc tgccgtgcgc tagccagagc gaccgcggga agtcgatcct cgaaagcatg
     6721 ccagacatgc cacgcgatcg ttgctattcc gtacgtaatc acggaccggt actcggtacg
     6781 ctggattgca cgcgtcgtat cggcagtttc ttcagttgcc tcgatgagcg agtgtgtgct
     6841 gaagtttgcc gtcacgattc caactaaggt cgtgggctcg tcgacgagga acacgggtga
     6901 gccgctcaag ccgggtgggg ctgcgaaact cagctcgaat gccggatatg acccgcgacc
     6961 tggagggtcg tagagaaacg tgcgctggat gtagcctctg aaacaacgga gcgtctccct
     7021 gtcggcatcc ggcgccagta ggtcaacggg ccaaccgatc gacgccacct cgctgccata
     7081 ccacgctgcg cccagtgcgt gggtgaaggg caggatccat cggggttcct ctacaactac
     7141 gtcgagaagt gcagcgtcca ggtgttgatc cacgacgatt tcgttgatcg ggctaaattg
     7201 atgcctcgag tggccgaagt ggttgacggc gatttcgtct agccttcggc cagccacgat
     7261 gtgtgcggcg gtgacgaaag tcgtcgcaga gccaaaggct gaagacgacc cgatgcagcg
     7321 cggcgtcgcg ctggacatgt cgaagacccc acctactgcc gacattcgag cgttgattct
     7381 ttcggtcatt tcaatctggc taaccttgta tgggtggagg gcaccaattg ctggttctct
     7441 ccacgaacga ctcgctccaa cgccgccccc gacgtaccct tcgggcgcta ttgtctgggg
     7501 cagtctggcg gcggtcaact ccttccaccc agcccgcgtg cgcgacgaca tcgagtgaca
     7561 acccacggat tggcgctggc gacacaaagc gcgctacgtc tggaatgggt gtgactctca
     7621 tcgtgatcaa gagacacgat ttcgcccggc cagccgcgtc tggcctggcg tctttcgaga
     7681 ggcgcgactg tcggcgtcgt gacacccgtg aggcagtaaa ccgggcccga aacgtgactg
     7741 ccgcgcagct ccacggagct gcgcgggtcg ctgaacccgc tgtagagggc ccggttttcc
     7801 acgacgtccg accggagcgc cgggtcgaaa tggcgccggc ccagacgcgg ttcgcggcga
     7861 aattcccttc atttgcccag aatcagatcc acgccctctg gaatgggtgg agggcgccaa
     7921 ttggtgctcc ccgacgacag ccctggtcgt cgtgacggca cgtggcggac caagagccct
     7981 taacgcggag ccctcgcacg tgcgacgagc gcgagaccgc gatccgcgtc cgcgtggctc
     8041 tccttcgcga gctgttgctt ctcggcgctc acgccggaga cgtagccggc gtcgccaacc
     8101 gacgcgaggt tcgtatccac gttcatctcc gcgccgtgga cggcggcacg cagcagctcg
     8161 atcgcgaccc cgacgtcgct cgccgcgctg ggcacgcatc tctcggccac ggccggcgcc
     8221 tcgccaagtg cccgccggca gaggcgcatc gtctcgagtg gagcctcggt cgccgaacgc
     8281 atcgcggcgt cgagcgcgtg ccgccgtgcg gccgcctgag tctcatcggc cttcggcagc
     8341 cgcagggcgc tcatca
//
LOCUS       DBNGSH010000647         5620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1086100, whole genome shotgun sequence.
ACCESSION   DBNGSH010000647 DBNGSH010000000
VERSION     DBNGSH010000647.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1086100"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="AC16CY_14960"
     CDS             complement(<1..246)
                     /locus_tag="AC16CY_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355147.1"
                     /translation="MAVRSTVKAFVLGIVAALAIVYLADNRIGPVQAQGPTRFAAVPS
                     EKGGQDIFGAYDVVADWPKDLSTIPGHEGWTFGAGQSV"
     gene            681..2234
                     /locus_tag="AC16CY_14965"
     CDS             681..2234
                     /locus_tag="AC16CY_14965"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355148.1"
                     /translation="MARRLLVIAALLALAIGDGQAQDARSVLQAASKAMGMANLKTIQ
                     YSGSGWTAAVGQSFSATTGDWPRFEVPSYTRTIDYDARSLRDEYTRRQGTFKPQGGGG
                     TPLQGEQRIVQLLSGTYAWNLQGETAAAQTRPYLDGTPVNELRQLEIMLTPHGFLKAA
                     MAANPRTHTLAIMGTSNDGLAGDGRKVTIVSFTALGKYKVNGTINDQNMVELVTTWIP
                     NPVYGDMLYEMRYTEYKDFNGLKFPTLLHVHQGDPVLNPAHNIQEIRVTSVQANVAVP
                     AIAVPDAVRKATVPPVTVETDKIAEGVWRIAGGSHHSVAVEFADWVAVVEAPLNEDRS
                     LAVIDAVTKLLPKKPIRYVVNTHHHFDHSSGLRTYLSQGTSIVTHQGNRDFYQQVMFY
                     PAPRTLQPDRLALFYPMYMTSRRPMRIETVTDRHVLTDKVRTLELHHVTGLDHVQGML
                     VAYLPAEKILINADLYSPPAAGAAAPVANASIRALAETVRRLKLDVTRHVGIHGAVGS
                     HEEFVKIGG"
     gene            2302..2709
                     /locus_tag="AC16CY_14970"
     CDS             2302..2709
                     /locus_tag="AC16CY_14970"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355149.1"
                     /translation="MIVKKTWPGVGVIVAAAAIVAAPATAHHGGATFDTTKTLTLQGT
                     VTEWIWANPHCFLKFDAKDETGTVRNWVVETGNPTDMSRSGWARTSFKVGDVVTVTLQ
                     PVKSGAPVGRIRGVVLPNGQKLGSQAPAPRPPQ"
     gene            2745..3527
                     /locus_tag="AC16CY_14975"
     CDS             2745..3527
                     /locus_tag="AC16CY_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355150.1"
                     /translation="MLKRLMVVAVAWAAGLAFFQVANAQVWNSHGMIGNEQPPQTGPA
                     PKRDMTGTWDAGGAGIAPRGSPTSALTAWGEKKVSAYRPGDGPRAALIGEINDPLSTM
                     CDPAGFPRNLLFELRPFQVVHTPNQVLMLYMFEKRFRTIWTDGRALPKDPDPRWYGYS
                     IGRWEDDFTFVVETIGMDEKTWLDNAGNPHSNDMRVEERYRRVNMGTMELTVTIDDPT
                     AYTKPWVARKGLPLRLIPSNVDLMEMICSATEAQEYKKNMGK"
     gene            3524..4321
                     /locus_tag="AC16CY_14980"
     CDS             3524..4321
                     /locus_tag="AC16CY_14980"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3355151.1"
                     /translation="MTPRLQSLARIVFGFLLLRHGMEQVFAYPEASNAAMLSYAGILE
                     LVALPAGVLIMAGLFTRRIALVLAAMYLVLYVVGPLQRGPFPHRNGGDPVLLNAFFFL
                     YLAAAGGGAWSLDRLRDPKGEAAADATWAPQALGLLRIAAGVLFLMHGLEKFFGVGGG
                     RIDRDIMTIRGLAGWLETIGGPLIVLGLFTRPVAFVLSGEMAVAYFRTWAPRGFWQSF
                     QQPGMEASILFCYLFLFMCAAGPGAWSLGDWLRARGGRARKSAHATA"
     gene            4410..5240
                     /locus_tag="AC16CY_14985"
     CDS             4410..5240
                     /locus_tag="AC16CY_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355152.1"
                     /translation="MRRTGLATLSLAVLLTVTIGAQWVNHPTQGIPRTPDGKPNLAAP
                     APKLPDGRPDFSGIWDVGNMTYFHDLGRGLKPGEIQLTPWAAGVQKVRRDRNHVDDPY
                     GYCLPLGVPRVYTRSPFKIVHTPSLTIVLHESFVGNTFRQIFTDGRPLPKLSETEPAW
                     LGYSVGRWEGDTFVVESIGFRDLGWLSANLSYPNSDALKVTERWKRADLGHLAITVTI
                     DDPKAYVKPWTNTIPLLLQADTELLDSYCDNQMTILKSWSFSKPEPEPPSPRENIVDR
                     "
     gene            5271..>5620
                     /locus_tag="AC16CY_14990"
     CDS             5271..>5620
                     /locus_tag="AC16CY_14990"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3355153.1"
                     /translation="MKGFVLFIAGLMVGAAIQTGVAQTQNPGIVGLNHVGLAVPDIPA
                     AAAFYTEKLGFREAFRNSNPQGQPTAIYMQASRDTFVELQQANEKQPAGLTHFGLVVA
                     NMKTAVDAFRARGAT"
BASE COUNT         1003 a         1817 c         1893 g          907 t
ORIGIN      
        1 cacgctctgc ccggcgccga aggtccatcc ttcgtgtcca gggatcgtcg agagatcctt
       61 cggccagtcg gcgaccacgt cgtaggcgcc gaagatgtcc tgcccgccct tttcactcgg
      121 cactgccgca aaccgcgtcg ggccctgtgc ctggacgggt ccgatccggt tgtcagcgag
      181 atacacgatc gcgagagccg cgacgattcc cagaacgaac gctttgaccg ttgaccgaac
      241 agccatatga gtcctccagc aggcccagat gttacaactc ccgccttcgc caaggctacg
      301 gcgaggctcg ccgtagcgcc ttcggcgcga aggcggccca acttctaact cccaactcct
      361 catcgccggc ccgtctgatg tgtcctgacg cgacggcgtg tcgcaagccc gtctcgcgaa
      421 agctcgccac tggcatcgca ggcgtgtgag gcgcggggga ggagcgaggg cggcaagcgc
      481 tcgcgacggc ggagcggcga cgaaccgtca ggcagacgcc ggagatgccc gtggccgggc
      541 gccgcgaccc gcggcgcgga aggacacgtc aggcggggcg gcggtgagtt agccggggcc
      601 gccgaccaga gtcggaagcc cagttatgag ttgacgttcg actgcaggcg cgttatgtaa
      661 cgctctcgac cggagggtat atggctcgaa ggctactcgt aatcgcggct cttctggcgc
      721 tggccatcgg tgacgggcag gcccaggacg cgcggagcgt gctgcaggcg gcgtccaagg
      781 cgatgggcat ggcgaacctc aagacgattc agtattcagg ctcggggtgg acggccgcgg
      841 tggggcagag cttcagcgcg acgacaggcg actggccccg cttcgaggtg cccagttaca
      901 cccggacgat cgactacgat gcccggtcgc tgcgcgacga gtacacgcgg cggcagggca
      961 ccttcaagcc gcagggcggc ggcggcacgc ctcttcaggg cgaacagcgg atcgtccagc
     1021 tcctgtcggg cacctacgca tggaacctgc agggcgagac ggcggctgcg cagacgcggc
     1081 cgtacctgga cgggactccg gtcaacgagc tgcgccagct cgagatcatg ctgacccctc
     1141 atggattcct caaggcggcc atggccgcca acccgaggac gcacacgctg gccatcatgg
     1201 gcacctcgaa cgacgggctc gccggcgacg gaaggaaggt cacgatcgtc tccttcacgg
     1261 cgctcgggaa gtacaaggtg aacggaacga tcaacgacca gaacatggtc gagctcgtga
     1321 cgacgtggat tcccaatccg gtgtacggcg acatgctgta cgagatgcgc tacacggagt
     1381 acaaggactt caacggactc aagttcccga cgctcctcca cgtgcatcag ggcgacccgg
     1441 tgctcaaccc cgcgcacaac atccaggaga ttcgcgtgac gagcgtccag gcgaacgtcg
     1501 ccgtgccggc gatcgccgtg ccggatgccg tgcggaaggc cacggttccc cccgtcaccg
     1561 tcgagaccga caagatcgcc gagggcgtgt ggaggattgc cggcggctcc catcacagcg
     1621 ttgccgttga attcgccgac tgggtcgcgg tcgtcgaagc gccgctgaac gaggaccgat
     1681 cgctcgccgt catcgacgcg gtcaccaagc tcctgccgaa gaagcccatc cggtacgtgg
     1741 tcaacacgca tcaccacttc gaccactcca gcgggctcag gacgtatctg tcccagggca
     1801 cctcgatcgt cacgcaccag gggaatcgcg acttctatca gcaggtgatg ttctaccccg
     1861 cgccgcggac gctgcagccg gaccggctcg cgctgttcta tccgatgtac atgaccagcc
     1921 gcaggcccat gcgcatcgag acggtgaccg accggcacgt gctgaccgac aaggtgcgaa
     1981 ccctggaact gcaccacgtg accggcctcg accacgtgca ggggatgctc gtggcgtacc
     2041 tgccggctga gaagatcctg atcaacgcgg acctgtattc gccgcccgcg gcgggcgcgg
     2101 ccgcgcccgt tgcgaacgcc tccattcgag cgctggccga gaccgtccgc cgcctgaagc
     2161 tggacgtgac ccggcacgtg gggattcacg gggccgtcgg ctcgcacgag gaattcgtta
     2221 agattggtgg ctgatagacc gaagcggtcc ggctgaagcc tgacactacg tacgcacggc
     2281 gaccatgaag aagggcggag ggtgattgtg aagaaaacgt ggcccggcgt cggtgtgatc
     2341 gttgcggcgg cagccatcgt cgcggctccg gccaccgctc atcacggcgg agcgacgttc
     2401 gacacgacaa agacgctcac gcttcaggga accgtgacgg agtggatctg ggccaacccg
     2461 cattgtttcc tgaagttcga cgcgaaggac gagaccggca cggtccgcaa ctgggtcgtg
     2521 gagacgggca atccgaccga catgtccagg agcggctggg cacggacctc cttcaaggtc
     2581 ggcgacgtcg tgaccgtcac cttgcagccg gtcaagagcg gggcacctgt cggtcgtatt
     2641 agaggcgtgg tgctgccaaa cggccagaag ctcggatcgc aggcgcccgc cccgcggccc
     2701 ccgcagtaga tccgtggtcg agtgttcacg ttgagggacc gaacatgctg aagcggttga
     2761 tggtcgtggc ggttgcgtgg gcggccgggc tggcgttctt ccaggtcgcc aacgcgcagg
     2821 tctggaacag ccacggcatg atcggcaacg agcagcctcc gcagaccggg cctgcgccca
     2881 agcgcgacat gaccggcacg tgggacgcgg gcggcgccgg tatcgcgcca cgtgggtcgc
     2941 cgacgtcggc cctgaccgcc tggggcgaaa agaaggtcag tgcttaccgg ccgggcgacg
     3001 gacctcgagc ggcgctgatc ggagagatca acgatccgct gtccacgatg tgcgatccgg
     3061 ccgggttccc gcgcaacctg ctgttcgagc tgaggccgtt tcaggtcgtg cacacaccca
     3121 accaggtgct gatgctctac atgttcgaga agcggttccg gacgatctgg accgacggcc
     3181 gcgcgcttcc gaaggatccc gacccccggt ggtacggcta ttcgatcggc cgctgggagg
     3241 acgacttcac gttcgtcgtc gagacgatcg ggatggacga gaagacgtgg ctcgacaacg
     3301 ccggcaatcc gcacagcaac gacatgcgtg tggaggagcg ctaccgccgc gtgaacatgg
     3361 gcacgatgga gctcaccgtc acgatcgacg acccgaccgc gtacaccaag ccgtgggtgg
     3421 cgcggaaagg gctgccgctg aggctgatcc cgtccaacgt cgatctcatg gagatgatct
     3481 gctcggcgac cgaggcgcag gaatacaaga agaacatggg gaaatgacac cccgtctgca
     3541 gagcctcgct cgcatcgtct tcgggttcct cctgcttcgg cacggcatgg agcaggtgtt
     3601 tgcctacccc gaggcgagca atgcggcgat gctgtcgtac gcggggatcc tcgagctcgt
     3661 ggctcttccc gcgggcgtgc tgatcatggc gggtctgttc acgaggcgga tcgccctcgt
     3721 gctcgcggcg atgtacctcg tcctgtatgt cgtcggcccg ctccagcgag gtccgttccc
     3781 gcaccgcaac ggcggcgatc ccgttctgct gaacgccttc tttttcctgt acctggccgc
     3841 ggccggtggc ggcgcgtgga gcctcgatcg gctgcgcgat cccaagggtg aggcagcggc
     3901 ggacgcgacg tgggccccgc aggcgctcgg cctgctgcgc atcgccgccg gcgtgctgtt
     3961 cctcatgcac ggcctggaaa agttcttcgg cgtcggcggc gggcggatcg atcgcgacat
     4021 catgacgatc cggggccttg ccggctggct cgaaacgatc ggcggaccgc tgattgtgct
     4081 cgggctgttc acgcgtcccg tcgccttcgt gctgtcggga gagatggcgg tcgcctattt
     4141 caggacgtgg gcgccgcgcg ggttctggca gagtttccag cagccgggca tggaagcgtc
     4201 gattctgttc tgttacctgt ttctgttcat gtgcgcggcc ggtccggggg cgtggagcct
     4261 cggcgactgg ctgcgtgcac ggggtgggcg cgcgaggaag agcgcacacg cgaccgcgta
     4321 gtaccgtagg ggcgggtctt tagacccgcc ctcggggaag catacctgta gggacgggtc
     4381 ttcagacccg ccctcagcgg aggagcgaaa tgagacgcac gggacttgcc acgttgagcc
     4441 tggccgtgtt gctgaccgtg acgattggcg cgcaatgggt gaaccatcca acgcaaggca
     4501 ttccccggac gcccgatggc aagccgaatc tcgccgcgcc ggcgccaaag ctcccggacg
     4561 ggcgcccgga tttctcgggg atctgggacg tgggaaacat gacctacttc cacgatctcg
     4621 gccggggcct gaagcctgga gagatccagt tgacgccgtg ggcggccggt gtgcagaagg
     4681 tccgccgcga ccggaatcac gtggatgatc cgtacggcta ctgcctgccg ctcggggtgc
     4741 cgcgcgtcta cacgcgcagc ccgttcaaga tcgtgcacac gccgtcgctc acgatcgtgc
     4801 tgcacgaatc gttcgtcggc aacacgttcc gccagatctt caccgacggc cgcccgctgc
     4861 cgaaactgag cgagaccgaa cccgcgtggc tcggatactc cgtcggccgg tgggagggcg
     4921 acacgttcgt cgtcgagagc atcggcttcc gcgatctcgg gtggctcagc gccaacctgt
     4981 cctacccgaa cagcgatgcg ctgaaggtca ccgaacgatg gaagcgcgcg gacctcggcc
     5041 acctggcgat caccgtcacg atcgacgatc cgaaggccta cgtgaagccg tggacgaata
     5101 cgatcccgct cctgcttcag gcggacacgg agctgctcga ctcgtactgc gacaaccaga
     5161 tgaccatcct gaagtcgtgg agcttctcga agcccgagcc cgagccgccg tcgccgcggg
     5221 agaacatcgt tgatcggtga tgtccgattg agattcagaa ggagcacatc atgaaaggtt
     5281 tcgtcctgtt catcgccggg ctcatggttg gcgccgcgat ccagaccggc gtcgcccaga
     5341 cccagaaccc cggcatcgtc ggcttgaacc acgtcggcct ggccgtgccg gacattcctg
     5401 cggcggcggc cttctacacc gagaagctgg gattccgcga ggcgttccgc aacagcaatc
     5461 cccaggggca gccgacggcg atctacatgc aggccagccg cgacacgttc gtcgagctgc
     5521 agcaggcgaa cgagaagcag cccgcaggcc tcacgcattt cgggctggtc gtcgcaaaca
     5581 tgaagacggc tgtcgatgcg ttccgggcgc gcggcgcgac
//
LOCUS       DBNGSH010000648         4042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_746751, whole genome shotgun sequence.
ACCESSION   DBNGSH010000648 DBNGSH010000000
VERSION     DBNGSH010000648.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_746751"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="AC16CY_14995"
     CDS             <1..1218
                     /locus_tag="AC16CY_14995"
                     /inference="COORDINATES: protein motif:HMM:NF019222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3355154.1"
                     /translation="PPRRFVGIWHPHGASPGYWSPLQEGKDFEFSYITRPLEPFRNRV
                     VLISGLDMPEAMATTDEPGGDHARGAVLLSGARPRRNAVSPFLGVTIDQHIANKYGQD
                     TILSSLQLGVEDTGNFGNCNWGYSCAYTNSISWSSPTTPLPTQVNPRVVFERLFGDGA
                     SPEERLRDRQRNASILDAVVGQLGAFKASLGAGDKARIETYVDNIRELERRIKIAMEN
                     AVKEPDSDVPFGLPQSRHEHLRLMYDLMALALEGDITRSMTYMLGRDLSGASFPESGF
                     NGGWHGSSHHGDKPENIANYAKVNRYHVQLLAYFCEKLSKIPDGDGTLLDHVLIYKGS
                     NMGNSHRHAHEKVPVILVGGIDGTFKGNRHLVFPDNTQRTSNMLLSILHLYGIEMDKI
                     GTSTGRLQPLEMA"
     gene            1215..1793
                     /locus_tag="AC16CY_15000"
     CDS             1215..1793
                     /locus_tag="AC16CY_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355155.1"
                     /translation="MKHFVAFVSFVAFVVPVFVSFVAPVSAQFGRTTRPQVVPPQGPT
                     RQVIFKYCTNCHGIDDYAYNALDRAAWDDLLTRKHRGLDVPLPSDARGVLLDWLAERF
                     GPTTKPFPRAYVAPEVTTFFSDAEANALLKRACTACHELSRVNDARFSPERWRVVTLD
                     MRERGAKLEDEELERLVEWLGRTKGTNDSDRQ"
     gene            1876..2493
                     /locus_tag="AC16CY_15005"
     CDS             1876..2493
                     /locus_tag="AC16CY_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355156.1"
                     /translation="MLAAGMLGAEEPVLKLRATVVGAAEAPLTIQLLRWSTDAERAPL
                     LAALSAPPPPPAASAVAETAAPARGRGGRAGRGGRGAAPPSSPAARLTAAVKAAPTVG
                     FIWGDGPTGYSIKYAWRSASPGAPSRVVLVTDRRLGAHSTSWPVTEVPATANEAEFTV
                     VEMRIDAKGLGEAKAAPASPVVDTAANTLALDGYDAAPVLLRVTR"
     gene            2490..>4042
                     /locus_tag="AC16CY_15010"
     CDS             2490..>4042
                     /locus_tag="AC16CY_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3355157.1"
                     /translation="MRRLTLSVLSILLLGVPVHAASTDVADAAMRGDGNAVRAALARK
                     ADVNAAQVDGSTALHWAAERDDLDMADQLLRSGARVAARTREGVTPLQLAAINGSAAM
                     VDRLLKAGADPNAPMGAAGETALMMTARTGKSGAIRVLVEAGANVNAKESWGGTTALM
                     WAVSEGHADAARLLIGAGADVNARSHYVAAANGRGFEGRTPIANRTDARPEDFASGWL
                     TPLMLAAREGDVGLARILLAAGADIDAIAGDGKTALSLAIFNGNYEVASFLVDSKADV
                     NIAEAQRFTPLFWAVDRRNMETAPNFPWMATADPMPLIRKLLDAGANPNALVNNTPRA
                     RMREGSPRIVFATALMRAAFAADMELVTLLLERGADPKVISRDGENMLEAAAGLGFIH
                     GYHRGKPDAERLQVVRKFVELGNDVNHPDDYGITPLMAAGNYGNVPVIQYLIDAGADL
                     GAHDLGKKNDGQFGSSNEPLMPIDYAIGVGTFVPNNAVIIHEDAVALMARYMKERGIA
                     HTTSECTLRG"
BASE COUNT          668 a         1378 c         1402 g          594 t
ORIGIN      
        1 cccccgcggc gcttcgtcgg catctggcat ccgcatggcg cgtcgccggg ctactggagt
       61 ccgctccagg aagggaagga cttcgagttc tcctacatca cccggccgct cgagccgttc
      121 cgcaaccggg tggtgctgat cagcgggctc gacatgcccg aggcgatggc caccaccgac
      181 gagccgggcg gcgaccacgc gcgcggcgcg gtgctcctgt ccggcgcgcg tccccggcgc
      241 aacgccgtca gcccgttcct cggcgtcacg atcgatcagc acatcgccaa caagtacggc
      301 caggacacga tcctgtcgtc gctgcagctc ggcgtggagg acacaggcaa cttcgggaac
      361 tgcaactggg gctacagctg cgcgtacacg aactcgattt cgtggtcgtc gcccacgacg
      421 ccgctgccga cgcaggtgaa cccgcgcgtc gtcttcgagc ggttgttcgg cgacggcgcg
      481 agcccggaag agcggctgcg cgatcgtcag cggaacgcaa gcatcctcga cgcggtcgtc
      541 ggccagctgg gtgcgttcaa ggcgagtctc ggcgccggcg acaaggcgcg catcgagacg
      601 tatgtggaca acatccgcga gctcgagcgg cgcatcaaga tcgccatgga gaacgcggtg
      661 aaggaacccg acagcgacgt gccgttcggg ctgccgcaga gcaggcacga gcacctgcgg
      721 ttgatgtacg acctgatggc gcttgccctc gagggcgaca tcacgcggtc gatgacctac
      781 atgctcggcc gcgatctgag cggcgccagc ttccccgagt cggggttcaa cggcggctgg
      841 cacggcagtt cgcaccacgg cgacaagccg gagaacatcg ccaactacgc gaaggtcaac
      901 cggtaccacg tccagctcct ggcgtacttc tgcgagaagt tgagcaagat ccccgacggc
      961 gacggcacgc tgctggacca cgtgctgatc tacaagggga gcaacatggg gaactcgcac
     1021 cgccacgcgc acgagaaggt gcccgtgatt ctcgtcggag gcatcgacgg gacgttcaag
     1081 ggcaaccggc acctggtgtt ccccgacaac acgcagcgca cgtcgaacat gctgctcagc
     1141 atcctgcacc tgtacgggat cgagatggac aagatcggaa cgagcaccgg gcgcctgcag
     1201 cccctcgaga tggcatgaag cacttcgtgg ccttcgtgag cttcgtggcc ttcgtggtcc
     1261 cggtcttcgt gtccttcgtg gccccggtct cggcgcagtt cgggcggacg acgcggccgc
     1321 aggtcgtgcc gccgcagggc ccgacgcggc aagtgatctt caagtactgc accaactgtc
     1381 acggcatcga cgactacgcg tacaacgcgc tcgatcgcgc cgcgtgggac gacctcctga
     1441 cgagaaagca ccgcgggctg gacgtgccgc tcccgtccga cgcgcgaggg gtgctgctcg
     1501 actggctggc ggaacggttc ggcccgacga ccaagccgtt cccgcgcgcg tacgtcgcgc
     1561 ccgaagtcac caccttcttc tccgacgccg aggccaacgc gctgctgaaa cgagcgtgca
     1621 cggcgtgcca cgagctgagc cgcgtcaacg acgcgcgctt cagccccgag cggtggcgcg
     1681 tcgtgacgct ggacatgcgc gagcgcggcg cgaagctgga ggacgaggaa ctggagcgcc
     1741 tggtcgagtg gctgggccgt actaaaggga ccaacgacag tgacaggcaa taagcggtcc
     1801 ttggtccttg gtccctggtc cttggtccgt ccttggtcct tggtccgtcc ttggtccttg
     1861 gtccttggtc cggtagtgct tgccgcaggc atgctcggcg ccgaggagcc ggtgctgaag
     1921 ctgcgcgcca cggtcgtcgg ggctgcggag gctccgctga cgattcagtt gcttcgatgg
     1981 tccaccgacg ccgaacgcgc accgttgctc gcggcgcttt cggcgcctcc tccgccgcct
     2041 gccgcgtccg ccgttgccga gaccgcggcg cccgcgcgcg gacgcggcgg ccgggccgga
     2101 cgcgggggac ggggtgcagc gccgccctcg agcccggccg cgcgcctcac ggcggccgtc
     2161 aaggcggcgc cgaccgtcgg tttcatctgg ggtgacggcc cgaccggcta ctcgatcaaa
     2221 tacgcgtggc ggtccgcgtc gccaggcgcg ccgtcgcggg tggtgctggt gaccgatcgg
     2281 cgcctcggcg cgcactcgac ttcctggccg gtcactgaag tcccggcgac ggcgaacgag
     2341 gcggagttca cggtcgtgga gatgcggatc gacgccaagg gcctcggcga ggcgaaggcc
     2401 gctccggcgt cccctgtcgt cgataccgcc gcaaacacgc tggcgctcga cgggtacgac
     2461 gcggctccag tcctgctcag ggtgacgcga tgagacgcct cacgctttcc gttctgtcga
     2521 tccttctgct cggcgttcct gtgcacgccg ccagcaccga cgtcgccgac gcggccatgc
     2581 gcggcgatgg caacgccgtg cgcgcggcgc tcgccaggaa ggcggacgtc aacgccgccc
     2641 aggtcgacgg cagtaccgcg ctgcactggg cggccgagcg cgacgacctc gacatggccg
     2701 atcagctgct ccgctccggc gcacgcgtcg ccgcgagaac cagggaaggg gtgacgccgc
     2761 tgcagctcgc ggccatcaac ggcagtgcgg ccatggtgga ccggttgctc aaggccggcg
     2821 ccgatccgaa cgcgccaatg ggcgcggcag gggagacggc gctgatgatg acggcgcgta
     2881 ccggcaagag cggcgcgatt cgcgtgctcg ttgaagcggg cgccaacgtc aacgcgaagg
     2941 aatcgtgggg cgggacgacg gctctgatgt gggcggtctc ggagggacac gccgacgcgg
     3001 cccggctgct catcggcgcc ggcgccgatg tgaacgcgcg atcgcactac gtggccgccg
     3061 ccaacggacg tggattcgaa gggcgcacgc cgatcgcgaa caggacggac gccaggcccg
     3121 aggacttcgc cagcgggtgg ctcacgccgt taatgctggc cgcccgcgag ggcgacgtcg
     3181 gtctggcgcg catcctcctc gcggcgggcg ccgatatcga cgcgatcgcg ggcgacggca
     3241 agaccgcgct ctcgctcgcg attttcaacg gcaactacga ggtggcgtcg ttcctcgtgg
     3301 acagcaaggc ggacgtcaac attgccgagg cgcagcggtt cacgccgctc ttctgggccg
     3361 tcgatcggcg gaacatggaa acggcgccga acttcccgtg gatggcgacc gccgatccga
     3421 tgccgctcat ccgcaagctg ctcgacgcgg gcgccaatcc aaacgcgctc gtcaacaaca
     3481 cgccccgggc ccgcatgcgc gaggggtctc cccggatcgt cttcgccacc gccttgatgc
     3541 gcgccgcgtt tgccgcggac atggagctgg tgacgctgtt gctcgagcgc ggcgccgatc
     3601 cgaaggtcat ctccagggac ggcgagaaca tgctcgaggc cgcggccggc ctgggattca
     3661 tccacggcta ccaccgcggg aagccggacg cggagcgcct gcaggtcgtc aggaagttcg
     3721 tcgagttggg caacgacgtc aaccacccgg acgactacgg gatcacgccg ctgatggccg
     3781 ccggcaacta cggaaacgtc cccgtgattc agtacctgat cgatgccggc gccgatctcg
     3841 gcgcccacga cctgggcaag aagaacgacg ggcagttcgg gtccagcaac gagccgctga
     3901 tgccgatcga ctacgcgatc ggcgtcggca cgttcgtgcc caacaacgcc gtcatcatcc
     3961 acgaagacgc ggtggcgctg atggcgaggt acatgaagga acgcggaatc gcgcacacca
     4021 cgtccgagtg cacgctgcgc gg
//
LOCUS       DBNGSH010000649         2944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2104881, whole genome shotgun sequence.
ACCESSION   DBNGSH010000649 DBNGSH010000000
VERSION     DBNGSH010000649.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2104881"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="AC16CY_15015"
     CDS             complement(<1..581)
                     /locus_tag="AC16CY_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3355158.1"
                     /translation="MRNKTYSTLFVVLLAVLIGLGALLTRGGSSSSADAPSIPFEKYT
                     LPNGLDVILSRDARIPMVAVNLWYHVGPANEEPGRTGLAHLFEHMMFQSSKHVPADSH
                     FKLLEAAGASDINGTTDYDRTNYFQTVPTNQLELALWLESDRMGYLLDVVDQTALANQ
                     QDVVRNERRQSVENQPYGLAEETLVQTLFPKGHP"
     gene            complement(571..1185)
                     /locus_tag="AC16CY_15020"
     CDS             complement(571..1185)
                     /locus_tag="AC16CY_15020"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR3355159.1"
                     /translation="MASIDYASARRLLARRDPVIAGLMRRHGDCGLETAQRSDHFRAL
                     IHAIIAQQLSTKAAATIEARFAAMFDGSPTPAAIARVSDEQFRSVGLSPQKLRYVRDL
                     CVHLLDGSLDLDSLEHLTDEDVVAALTSVKGIGRWTAEMFLMFRLHRPDVLPVGDLGI
                     VKAVKQAYRLRSRPTPARITKIGEMWRPYRSVACWYLWASLDAK"
     gene            complement(1211..1900)
                     /locus_tag="AC16CY_15025"
     CDS             complement(1211..1900)
                     /locus_tag="AC16CY_15025"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR3355160.1"
                     /translation="MKYAPLAILLLLAPSATSAQPPAAVPTVVVTGEGVVHAVPDRAW
                     IRIGAESRASNPREAQRLNSEAMRPVQEKLKAADVAPDAIRTVGYDVQYEWDIVNNKR
                     VGRGYVARNTIEVRVDAVDRVGEYLELAVGSGATSLGGIRFDLKDQTKLEREAMRLAV
                     TDARSKAEAAAEGAGRAIDRIVRIEEQGAAPPMPIQARTFAAQAADSSPPIAAGQMEI
                     RARVTLTAALK"
     gene            1982..>2944
                     /locus_tag="AC16CY_15030"
     CDS             1982..>2944
                     /locus_tag="AC16CY_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3355161.1"
                     /translation="MPPSGPLAGVTVLDLTRVLSGPYCTMLLADMGARVIKLERPGDG
                     DETRAWGPPFAGGESAYFLGINRNKESVTLDFKRPEGRKIIDRLLGTSDVFIENFRPG
                     ALARAGLDYASLRPDYPRLVYASISGFGQTGPKRHQPGYDAVIQAEGGLMSVTGDPDG
                     PPFRVGVAIADLVAGLLAAQGVVLALYARERTGRGQYVDISMLDGIVSVLSYHASMYL
                     TTATRSSRLGNRHATIAPYDTFTAADGEFFLAVGNDDQFRRLCQAAGLASLCDEAAFA
                     TNPARVVHHAELRARLAPALRSRPRAYWIEILTEAGVPSGAVRDV"
BASE COUNT          470 a          977 c          997 g          500 t
ORIGIN      
        1 ggatggccct tggggaagag cgtctgcacc agcgtttctt ccgcgagccc atagggctgg
       61 ttctccacgc tctgacggcg ttcgttccgt acgacgtcct gctggttcgc aagcgctgtc
      121 tggtcgacga cgtcgagcag gtagcccatg cgatccgatt ccagccacaa ggcgagctcc
      181 agctgattgg tcggaaccgt ctggaagtag ttggtacggt cgtagtcagt cgtgccgttg
      241 atgtcgctgg cgccggccgc ctcgagcagc ttgaagtgcg agtccgcggg aacgtgcttc
      301 gagctctgaa acatcatgtg ctcgaagaga tgcgctaagc cggttcgccc tggctcttcg
      361 ttggcggggc caacgtgata ccagaggttc acggccacca tgggaatgcg cgcatcgcgg
      421 ctcaggatca cgtcgagccc gttcgggagc gtgtacttct cgaacggaat gctcggcgcg
      481 tcggcggacg aggacgatcc gccgcgggtg agcagcgccc ccaggccaat caggacggcg
      541 agcaggacga cgaaaagggt cgagtaggtc ttatttcgca tcgaggctcg cccaaagata
      601 ccagcaggcc accgatcggt acggccgcca catctctcca atcttcgtga tccgcgcggg
      661 cgtcggccgc gaacgcagtc tatacgcctg cttgaccgcc ttcacgatgc cgaggtcgcc
      721 gaccggcagg acgtcggggc ggtgcagccg gaacatgagg aacatctccg ccgtccagcg
      781 gccgatgccc ttgacgctcg tcagcgcggc gacgacgtcc tcgtcggtga gatgctcgag
      841 cgaatcgaga tcgagcgagc cgtccagcag gtgcacgcac aagtcacgga cgtatctgag
      901 cttttgaggg ctgaggccga cggatcgaaa ctgctcgtct gagacacggg cgatggccgc
      961 cggcgtgggg cttccgtcga acatggccgc gaagcgcgcc tcgatggtgg ccgcagcctt
     1021 cgtggacagc tgctgggcga tgatcgcgtg gatcagggca cgaaaatgat cgcttcgctg
     1081 cgcggtttcc aggccgcagt ctccgtggcg ccgcatcagt ccggcgatca ccggatcgcg
     1141 gcgcgcgagc agacgccggg cgctggcgta gtctatcgag gccatcggcg gcgccaattc
     1201 tacccggcct tcacttgagg gcggccgtca acgtgacgcg ggcgcgaatc tccatctggc
     1261 cggccgcgat cggcgggctg ctgtcggccg cctgcgcggc gaacgtgcgg gcctgaatcg
     1321 gcatcggcgg cgcggctccc tgctcttcga tgcgtacgat ccggtcgata gcgcggcccg
     1381 cgccctcggc ggcagcttcg gccttggagc gcgcgtcggt taccgccagc cgcatcgctt
     1441 cccgctcgag cttggtttga tccttcagat cgaaccggat gccgcccagc gaggtggcgc
     1501 cggacccgac cgcgagctcc agatactcgc cgacgcggtc cacggcgtcc acacgcactt
     1561 cgatcgtgtt gcgggcgacg tatccccgcc cgacacgctt gttgttgacg atgtcccact
     1621 cgtactgcac gtcgtagcca accgtccgaa tggcgtccgg ggcgacgtcg gcggccttca
     1681 gcttctcctg cacgggcctc atcgcctcgc tgttgaggcg ctgcgcctcg cgcggattcg
     1741 aggctcggga ctccgcgccg attcggatcc aggcgcgatc cggcacggcg tggacgacgc
     1801 cttcacccgt cacgacgacc gtcggcacgg cggcgggcgg ttgcgcggag gtcgcggatg
     1861 gagcgagcag cagcagaatc gccagcggtg cgtacttcac ggggcctcct tgtcaggtcc
     1921 agacacagat tctgactcga aacgcacggc ccaggttgca atcactagaa tgaagggcct
     1981 gatgcctcca tccggacccc tggccggcgt gacagtgctc gatctcacgc gcgtgctgtc
     2041 aggcccgtac tgcacgatgc tcctggccga catgggggcg cgcgtgatca agctcgagcg
     2101 cccgggagac ggcgacgaga cgcgggcgtg ggggccgccc ttcgcgggcg gcgagagcgc
     2161 gtacttcctc ggcatcaacc gcaacaagga aagcgtgacg ctcgacttca agcggccgga
     2221 gggccgcaag atcatcgacc gccttctcgg cacgagcgac gtcttcatcg agaacttcag
     2281 gccaggcgcg ctggcccgtg ccggcctgga ctacgcttcg ctgcgccccg attacccgag
     2341 gctggtgtac gcgtcgatct cgggatttgg acagaccggg ccgaaacgtc atcagcccgg
     2401 ctacgacgcc gtgattcagg ctgaaggcgg gctgatgagc gtgaccggcg atccggacgg
     2461 accgcccttt cgcgtcggtg tggccattgc cgacctggtc gcgggcctgc tggcggctca
     2521 gggcgtcgtg ctcgcgctct acgcgcgcga gcggaccggc cgcggacagt acgtggacat
     2581 cagcatgctc gacgggatcg tgtcggtgtt gagctaccac gcatcgatgt acctgacgac
     2641 ggcaaccagg tccagccgcc tcggaaaccg tcacgcgacg attgcaccct acgacacgtt
     2701 caccgcggcc gacggagagt tctttcttgc tgtcggcaac gacgaccagt tccggcgcct
     2761 gtgccaggcg gcagggctcg cctcgctgtg cgacgaggcc gctttcgcca ccaatcccgc
     2821 tcgcgtcgtc caccacgccg agctgcgcgc gcgccttgcg ccggcgctcc gatcccggcc
     2881 gcgggcgtac tggatcgaaa tcctgacgga ggcgggcgtg ccctcgggcg cggtgcgaga
     2941 cgtc
//
LOCUS       DBNGSH010000650         8348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2614764, whole genome shotgun sequence.
ACCESSION   DBNGSH010000650 DBNGSH010000000
VERSION     DBNGSH010000650.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2614764"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="AC16CY_15035"
     CDS             complement(<1..1301)
                     /locus_tag="AC16CY_15035"
                     /inference="COORDINATES: protein motif:HMM:NF019157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR3355162.1"
                     /translation="MTSLNLPNVKVTMAQQVAAGAFTPGGGGPGGRGFATLPAFCRVA
                     ATLTPSSDSDIKIEVWLPVSGWNGKFQAVGNGAWQGSIGYAAMAEALRRGYATTSTDT
                     GHVGGSASFGLGHPEKVIDFAYRSEHEMTVTAKAIVNRFYGSAPKYSYWVGCSAGGRQ
                     AMKEAQMFPADFDGIIAGSPGLDWSGRTAQAVRIAQAMQREESRIPAAKAQVLHSAVV
                     AACDGLDGVKDGLIEKPTACRFDPGVLACKGGDEPGCLTLAQVESAKLIYSPIVDAKN
                     KREIPGLAPGSELGWTDQGWTAGARATGLDHLRFLIFQDPLWDITKFNLAADVARLTE
                     GPSGAIDARDPNLKPFFDRGGKLLQYHGWSDPQITPLASTQYFDRVAAALGGASKVHS
                     SYRLFVAPGMGHCGGGEGPNDFDELAALEQWVENGKPPDTIV"
     gene            1651..2856
                     /locus_tag="AC16CY_15040"
     CDS             1651..2856
                     /locus_tag="AC16CY_15040"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR3355163.1"
                     /translation="MPAIFISYRRDDSGGHAGALARELGHRFGAENVFLDLDDIRGGA
                     DFVTTLEQALGRCDVLIAVIGRQWQAAADAQGRRRLDVPRDVVRMEIAAALRRNVAVI
                     PVLVEDAPMPSASDLPEDLQPLAVRNAMHLRNASWQHDVDKLADELRAVFYSPRPGMA
                     DPPIPPDFRPGNVFDRRKGLLVGLFAAIGVVFTGIGGWSFVETRRFIAHAAVADGRVI
                     ENVPDGGTYRAIVRFEPPAGAPVTFRSSAGSDPPSYGVGEIVRVLYDTADPSDARISS
                     WFSLWGFATIFSALGVAGLAVSAGLIATAVRRHRRQLALIRSGHPIMTVFDSVEQNVK
                     FTYNGRHPYRIVTKWRNPISGEIATFRSQDLWDDPTEQVKHRSITVLVDPGQLSNYVM
                     DVSMVRKAK"
     gene            complement(2973..3863)
                     /locus_tag="AC16CY_15045"
     CDS             complement(2973..3863)
                     /locus_tag="AC16CY_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355164.1"
                     /translation="MRHSGTGRSGARGGAMSAGWRSRAGAVLLLAAAVCAPATIDAQW
                     VKHRTPGIPRTAAGTPNLAAPAPRTADGKPDFSGMWITAPANALPCGRGLVECGIELP
                     MSREGGYMGASLPGGLPYQPWAEELVKKNMATSSKDDPHGLCLPDTFLRAYGLPHIVK
                     FVQTPQLLIALEEYNVDYRQIFLDGRALPEDPTHSWKGYSVGRWEGDTLVVDSIGFRD
                     DLWLDFRGSPLTSAGKVRERITRPDFGHLEIDVTVDDPKAYTRPWTVRLQQQIIVDTE
                     TIDEVCLENEKFMRRLRGTP"
     gene            complement(3815..4576)
                     /locus_tag="AC16CY_15050"
     CDS             complement(3815..4576)
                     /locus_tag="AC16CY_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355165.1"
                     /translation="MIATAMSESAMGQGRGGGSGNAREGALIDLTGQWVSVITEDWRW
                     RMVTPPKGDTASVPLNPVGRKVAEAWDLNADRAGGRLCKAFGPPGLIRQPGRLRIRWE
                     NDSTLVMESDAGTQTRRFHFGRTSPVGARTLQGHSEARWFRQPQNRGIFGRGGVARGG
                     ALEVVTTNLTAGYLRPNGVPYSERAVVKEFFDSFTLPEDGTWLIVTTVVQDPEYLTQE
                     FVISSQFRKETDPSKWNPRPCDIPAPVAPARAAAR"
     gene            complement(4621..5559)
                     /locus_tag="AC16CY_15055"
     CDS             complement(4621..5559)
                     /locus_tag="AC16CY_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355166.1"
                     /translation="MTKRLVRWCVAVLVVAAPSVIEAQGDLTVLPAQGSLYVLVGPGG
                     NSAVQVGAEGPLMVDTQPAAMSARVLEAVRGLSPRPIRHVVLTSGGDQQAGGAATISK
                     AGRYVRVIDSIDPRGLDVRASIIAHLNVLNRMSAGSVPSDSWPTDTYFTPEWSIFSNG
                     EAVQLFHLAAATSDGDTIVFFRRSDVVCTGAIFDPTGYPRFDPERGGTIAGIIEGLNR
                     VLDLTVPGENQEGGTVVIPGRGRLSDETDVANYRDMVTIIRDRIRAMVGKGLSLEQVK
                     RARPTMDYDGLFESRSDWTSDMFIDAIYRDLRRSKP"
     gene            complement(5556..6407)
                     /locus_tag="AC16CY_15060"
     CDS             complement(5556..6407)
                     /locus_tag="AC16CY_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355167.1"
                     /translation="MSPRQLVSCLALAAGLALGGTVLAHVELSGGWGQRFHEDLPERG
                     AGPEIGDYLGLPINDAARLRADSWDAGKWTMLERQCEPHPADYGPRGPASMRITSTVD
                     PVSQDVISWHTTLMWMLPHRTIYMDGRPRPSAYAQHSWQGFSTGEWEGDMLKVTTTHL
                     KEGWLRRNGVPRSDKATLIEYFIRHGDYLTLVTIVKDPVYLTEPFVRTSNWVADPGFQ
                     LSPFSCIPTLEIERARDEVPHNLPGENRYLSEFAGKHGLPVDAARGGAMTMYPDYLTQ
                     LRAVDRK"
     gene            complement(6413..>8348)
                     /locus_tag="AC16CY_15065"
     CDS             complement(6413..>8348)
                     /locus_tag="AC16CY_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3355168.1"
                     /translation="TKFRKIVVTDDRGRYLLPDLPAKATFTVWVRGYGLIDSSPVRSQ
                     PGRALALTAIVAPDARTAAGVYPANYWYSLIDVPAEKEFPGTGEAGNGIAPEMRTQHH
                     WINQIKANCNVCHQMGNQATREIPKALGTFASSMAAWDTRVQVGQDGQGMSNMVTGLG
                     RRRGLEMFATWTDRVSRGDIPPVPPRPQGVERNLVLTMWDWGGPATFAHDELTTDKRN
                     PTANAYGPIYGVDWGNDGFLIVDPLEHTAQELRIPVLDPKVPPGKAQSMPVPSPYWGN
                     KLYWFDPAITNHAAMDAKGRVWMSSRFRRPEDQPAFCGTHPSAALAPQKSSFRQVQYF
                     DPNTRQFKQVNICFDTHHVQFAGDKDETLYGNGVFSGAIGWINTRILDETGDEAKAQG
                     WCRGYHDLNQDGKVDPAVDRQIAVSVIYSVIPHPTDGTVWGAAPGPMPGKIIRIDPKT
                     CVGEAYEPPFDPAAGVVGYTPRGIDVDSNGVIWTALASSGHLASFDRRKCKNALQGPS
                     ATSGQHCREGWTLHPVPGPRFRGVRGDVAVDFQYYNFVDRFDTFGLGANTPFANGTNS
                     DSLLALRPDGTWLVFRVPYPLGFFSRGMDGRIDDPKAGWKGRAIYADYGPNAVWHMEG
                     GLGTRSAVVKFQLRPDPLAR"
BASE COUNT         1333 a         2847 c         2708 g         1460 t
ORIGIN      
        1 acgatcgtgt cgggtggctt cccgttctcg acccactgct cgagcgccgc cagctcgtcg
       61 aagtcgttcg gcccttcgcc gccgccgcag tgccccatgc caggcgccac gaacagccgg
      121 taggagctat gcactttgga cgcgccgcca agcgcggcgg ccacacggtc gaagtactgg
      181 gtgctggcga gcggagtaat ctgcgggtcg ctccagccgt ggtactgaag cagtttgccg
      241 ccgcggtcga agaacggttt caggttcgga tcgcgcgcgt cgatggcgcc gctggggccc
      301 tcggtcagtc gcgccacgtc ggccgccagg ttgaacttcg tgatgtccca tagaggatcc
      361 tggaagatca ggaaccggag gtgatcgagc ccggtcgcgc gcgcccctgc ggtccatccc
      421 tggtctgtcc acccgagctc gctgcccggc gccagacccg ggatctcgcg cttgttcttc
      481 gcatccacga tcggggagta aatcaacttc gcgctctcga cttgcgcgag cgtcaggcat
      541 cccggctcgt ccccgccttt gcacgccagc acgcctggat cgaatcggca cgcggtcggc
      601 ttttcaatca gcccgtcctt gacgccgtcc aggccgtcgc acgccgcgac gaccgcgctg
      661 tgcaggacct gcgccttggc ggcgggaatc cgggactctt ctctttgcat cgcctgcgcg
      721 atgcgaacgg cctgcgccgt gcggcccgac cagtccaggc ccggcgatcc cgcgatgatg
      781 ccgtcgaagt cagccggaaa catctgtgct tccttcatcg cctgccgtcc gccggcggag
      841 cagccaaccc agtacgagta cttgggggcg ctgccgtaga acctgttgac gatcgccttt
      901 gccgtgacgg tcatctcgtg ttcggatctg taggcgaaat cgatcacctt ctcggggtgg
      961 ccgagcccaa agctggcgct tcctcccacg tgccccgtgt cggtcgacgt cgtcgcatag
     1021 ccgcggcgta gcgcctcggc catcgccgca tagccaatcg atccctgcca ggcgccgttc
     1081 cccacggcct ggaactttcc gttccatccg gatacgggca gccagacttc gatcttgatg
     1141 tccgagtcgc tcgacggcgt gagcgtcgcg gcgacgcggc agaaggcggg cagcgtggcg
     1201 aagccgcgtc caccgggtcc accgcctcca ggcgtgaacg cacccgcggc gacctgctgc
     1261 gccatcgtca ctttcacatt ggggagattc agcgaggtca tctcctcgca ggaggccgcg
     1321 tgggcgtgtg cggcgggcca gagacaaagg atcgccagtg ccgatgcaca agaactcact
     1381 ttcctcatac gtcacctgct cccatgagtc gcgggggctg aagcccccgc tctaccgagg
     1441 ccttgccgaa gcccccgctc taccgagacc ttgccgaagc ccccgctcac cgagaccttg
     1501 ccgaagcccc cgctctacga agacgtgcga tggcacacca ggcttgcggc gccgcagtgt
     1561 ggcaccggcg atgggactcc gtcaatcaac ggaactcatg ccgcggacat gcctagaaag
     1621 gccgggctgt ggcagcatag ccactgggcc atgccggcga tctttatcag ctatcgcagg
     1681 gacgacagtg gaggtcatgc aggggcgctc gcgcgcgagc tcggccaccg tttcggcgcg
     1741 gagaacgtct tcctcgatct cgacgacatc cggggcggcg cggacttcgt caccaccctc
     1801 gagcaggcgc ttggccggtg cgacgtgctg attgccgtga tcggccgcca gtggcaggcc
     1861 gcggccgatg cgcagggacg ccgccgcctc gacgtgcctc gcgatgtcgt gcgcatggag
     1921 attgctgcgg ccttgcgccg caacgtcgcc gtgattccgg tgctggtcga ggacgcaccg
     1981 atgccctcgg cgagcgatct gccggaggat ctgcagccgc tcgctgttcg caatgcgatg
     2041 cacctccgca atgccagctg gcagcacgac gtggacaagc tggccgacga gctgcgcgcc
     2101 gtgttctact ctccgcggcc ggggatggcg gaccctccga ttccaccgga cttccggccc
     2161 ggcaacgtgt tcgatcgcag gaaaggcctc ctcgttggtc tcttcgccgc gatcggcgtc
     2221 gtgttcaccg gcatcggcgg gtggtccttc gtggagactc gccggttcat cgcgcatgcg
     2281 gcagtggccg acggacgcgt gatcgagaac gtgcccgacg gcggcacgta tcgtgcgatc
     2341 gtcaggttcg agccacccgc cggagcgccg gtcacctttc gctcgagcgc gggcagtgat
     2401 ccaccgtcgt acggcgtggg agagatcgtc cgcgtgctgt acgacacggc cgacccgtcc
     2461 gacgcgcgaa tctcgtcgtg gttttcgctg tgggggttcg caaccatctt ctcggcgctt
     2521 ggcgtcgccg gccttgcggt ctcggcgggc ctgatcgcga cggccgtgcg tcggcatcgg
     2581 cgtcagctcg ccctgatccg atcgggccac ccgatcatga ccgtcttcga cagcgtcgag
     2641 cagaacgtca agttcaccta caacggccgc catccgtacc gcatcgtgac gaagtggcgg
     2701 aatccgatct cgggagagat cgcaaccttc cgcagtcagg acctgtggga cgacccgacc
     2761 gagcaggtga agcatcgttc catcaccgtg ctcgtggatc cgggtcagct ctcgaactac
     2821 gtgatggacg tgtcgatggt gcggaaggcg aagtgaccgg cgcgcaccat atcgcttcgg
     2881 ctgcaaggac ccatatatat atcgagcgaa gaatgttggt caggtcaaaa gggacgtcat
     2941 gaggaacgtc ccggcggatc ggcctcctga tcctacggcg tcccgcgcag gcgccgcatg
     3001 aacttctcgt tctcgagaca gacctcatcg atggtctccg tgtccacgat gatctgctgc
     3061 tggaggcgga cggtccacgg ccgcgtgtac gccttcgggt cgtccacggt cacgtcgatc
     3121 tcgagatgac cgaagtccgg acgagtgatc cgctcgcgga ccttgccggc actcgtcaac
     3181 ggactgccgc ggaagtcgag ccacaggtcg tcgcgaaacc cgatggagtc cacgacgagc
     3241 gtgtcgccct cccagcgacc aactgagtac cccttccacg agtgggtggg atcctccggc
     3301 agcgcacgac catcgaggaa gatctggcgg tagtcgacgt tgtactcctc gagcgcgatg
     3361 aggagctgcg gcgtctggac gaatttgacg atatgcggca ggccatacgc gcgcaggaac
     3421 gtatccggca ggcacagccc gtgcgggtcg tccttggagc tcgtcgccat gttcttcttg
     3481 acgagctcct ccgcccaggg ctggtacggc aggccgccgg gaaggctggc gcccatgtag
     3541 ccgccctcgc gcgacatcgg cagctcgatt ccgcactcaa ccagcccccg tccgcacggc
     3601 agcgcgttgg ccggcgccgt gatccacatg ccggagaagt cgggtttgcc gtcggcggtc
     3661 ctcggtgcgg gcgcggcaag attcggcgtt ccagcggctg tgcgcggtat gcccggcgtg
     3721 cgatgcttca cccactgcgc atcgatcgtc gcaggagcgc agaccgcggc tgccagcagc
     3781 agcaccgcac cggcccggga gcgccagccg gcgctcatcg cgccgccgcg cgcgccggag
     3841 cgaccggtgc cggaatgtcg catggacgcg gattccattt cgacgggtcc gtctccttcc
     3901 tgaactgcga gctgatcacg aactcctgcg tcaggtattc agggtcctga accaccgtgg
     3961 tcacgatcag ccacgtcccg tcctccggca gggtaaagct gtcgaagaat tccttcacga
     4021 ccgcacgctc gctgtaggga acgccgttcg gacgcaggta gccggcggtg aggttcgtcg
     4081 tcacgacttc gagcgcgccg ccacgcgcca cgccgccgcg gccgaagatt ccgcgattct
     4141 gcggctgccg gaaccaccgc gcctcggaat gcccttgcag ggttcgcgcg ccgactgggc
     4201 tcgttcgtcc gaagtggaag cggcgagtct gggttcccgc gtcggactcc atgacgagcg
     4261 tggaatcgtt ctcccaccgg atgcgcagcc gaccgggctg gcgaatcaat cctggcggcc
     4321 cgaacgcctt gcataaccgg ccgccggcgc gatcggcgtt caggtcccag gcttccgcaa
     4381 ccttccgtcc gacggggttc aacggcacgc tcgcggtgtc gcccttcggc ggcgtgacca
     4441 ttcgccaccg ccagtcctcg gtgatgaccg acacccactg cccggtgagg tcgatcaacg
     4501 cgccctcgcg cgcgttgcca ctccccccgc cgcgaccttg tcccatggcg gactcggaca
     4561 tcgctgtcgc gatcatcagc acagccaccg ccgcggaaca tgcccgaccg cgccgcagcg
     4621 tcatggcttc gaccggcgga gatcgcggta gatggcgtcg atgaacatgt cgctcgtcca
     4681 gtcgctccgg ctttcgaaca acccgtcgta gtccatcgtc ggtctggcgc gcttgacctg
     4741 ctccagcgac agtcccttgc cgaccatcgc gcggatgcga tcgcggatga tcgtcaccat
     4801 gtcgcggtag ttggccacgt cggtctcgtc gctcagcctc ccgcgtcccg ggatcacgac
     4861 cgtgccgccc tcctggttct ctcccggcac ggtgagatcc agcacgcgat tcagcccctc
     4921 gatgatcccg gcaatggtgc cgccgcgctc gggatcgaag cgcggatatc ccgtcggatc
     4981 gaagatcgct ccggtgcaga cgacgtcgga gcggcgaaag aagacgatcg tgtcgccgtc
     5041 gctggtggcc gcggccaggt gaaacagctg cactgcctca ccgttcgaga agatcgacca
     5101 ctccggcgtg aagtacgtat cggtcggcca ggagtccgag ggaactgagc cggcgctcat
     5161 ccggttcagc acgttgaggt gcgcgatgat cgacgcgcgc acgtcgagac cacgcgggtc
     5221 gatcgagtcg atgacgcgga cgtaacgacc cgccttggat atggtcgccg cgccgccggc
     5281 ctgctggtcg ccgccgctgg tgagcacgac gtgccgaatc ggacgagggc tcaggccgcg
     5341 tacggcttcg agcacccgcg cgctcatggc cgcgggctgc gtatcgacca tcagcggacc
     5401 ctcggccccg acttgcaccg ccgagttgcc gccgggacca acgagcacgt agaggcttcc
     5461 ctgagccgga aggactgtga gatctccctg agcctcgatg acactcggcg ccgctaccac
     5521 cagcacggcc acgcaccacc tgacgagtcg tttcgtcact tgcggtccac cgcgcgcagt
     5581 tgtgtcaggt agtccggata catcgtcatc gcaccgccgc gtgcggcatc cacgggcagc
     5641 ccgtgcttgc cggcgaactc actgagatac cggttctcgc cgggcagatt gtgcggcacc
     5701 tcgtcgcgcg cccgttcgat ttcgagcgtc gggatgcacg agaacgggct cagttgaaag
     5761 cccggatcgg ccacccagtt cgacgtgcgc acgaacggct cggtgagata caccggatcc
     5821 ttgacgatgg tcacgagggt cagatagtcg ccgtggcgga tgaagtactc gatgagcgtg
     5881 gccttgtcgc tccgcggcac gccgttgcgc cgcagccagc cctccttcag gtgagtggtc
     5941 gtgaccttga gcatgtcgcc ttcccactcg ccggtcgaga agccctgcca gctatgctgc
     6001 gcgtacgctg acggacgcgg acgtccgtcc atgtagatcg tccggtgcgg aagcatccac
     6061 atcagggtgg tgtgccagga gatcacgtcc tgcgagaccg gatcgacggt gctcgtgatc
     6121 cgcatgctcg ccggaccgcg cggcccgtag tcggccggat gcggctcgca ctgccgctcg
     6181 agcatcgtcc actttccggc gtcccacgag tcggcacgca ggcgcgccgc gtcgttgatc
     6241 ggcagcccga ggtagtcgcc gatctcgggg ccggcgccgc gttcgggaag gtcttcgtgg
     6301 aaccgctgcc cccagccgcc cgagagctcg acgtgggcga ggacggtgcc gccgagcgcg
     6361 aggccggcgg cgagcgcgag acagctcacg agctggcggg gagacatggt ggctaccgtg
     6421 ccagcggatc gggccgcaac tggaacttca cgacggcgct ccgagtgccc agaccgcctt
     6481 ccatgtgcca caccgcattc ggtccatagt cggcatagat cgcccggcct ttccagccgg
     6541 cctttggatc gtcgatgcgg ccgtccatgc cgcgcgagaa gaatccgagc ggatacggca
     6601 cgcggaacac cagccaggtt ccgtccggcc gcaacgccag cagcgaatcg gagttcgtgc
     6661 cattcgcgaa cggcgtattg gcgccgagac cgaaggtgtc gaagcggtcc acgaagttgt
     6721 agtactggaa gtccactgcg acgtcgcctc gcacgcccct gaatcgcggc ccaggcaccg
     6781 gatggagcgt ccaaccctca cggcagtgtt gtccgctggt cgccgatgga ccctgcaggg
     6841 cattcttgca cttgcggcgg tcgaagctgg cgagatgccc gctgctggcc agcgccgtcc
     6901 agatcacgcc gttggaatcg acgtcgattc cgcgcggcgt gtagccgacg acgcccgccg
     6961 cgggatcgaa cggcggctcg tacgcttcgc ccacgcacgt ctttggatcg atgcgaatga
     7021 tctttcccgg catcgggccc ggcgccgcgc cccacactgt gccgtcggtc ggatgcggaa
     7081 tcacgctgta gatgacgctc accgcgatct gacgatcgac ggcgggatcg accttgccgt
     7141 cctgattcag atcgtgatag ccgcgacacc agccttgcgc cttggcctcg tcgccggtct
     7201 cgtcgaggat gcgcgtgttg atccagccga tcgcaccgct gaagacgccg ttgccgtaca
     7261 gcgtttcgtc cttgtcgccg gcaaactgca cgtggtgcgt gtcgaagcag atgttcacct
     7321 gcttgaactg ccgtgtgttc gggtcgaagt actgcacctg ccggaagctg ctcttctgcg
     7381 gcgcgagggc tgccgacgga tgcgttccgc agaacgccgg ctggtcctcg ggcctgcgga
     7441 agcgcgacga catccacacc cgtcccttgg catccatcgc cgcgtgattg gtgatcgcgg
     7501 ggtcgaacca gtacagcttg tttccccaat acggcgaggg tacgggcatc gactgcgcct
     7561 tccccggcgg taccttcggg tcgagcaccg ggatacgcag ttcctgcgcc gtgtgctcca
     7621 gcggatcgac gatgaggaag ccatcgttgc cccagtcaac gccgtagatc ggcccgtagg
     7681 cattggcagt ggggttacgc ttgtcggtcg tcagctcgtc gtgcgcgaac gtcgccgggc
     7741 cgccccagtc ccacatggtc agcacgagat tgcgctcgac accctgcgga cgaggcggca
     7801 ccggcgggat gtcgccgcgc gagacacggt ccgtccaggt ggcgaacatc tcgagacccc
     7861 gtcgtcgtcc caggccggtc accatgttgc tcatgccctg gccatcctgc ccgacctgga
     7921 cgcgtgtgtc ccatgccgcc atgctcgacg cgaatgtccc gagcgctttc gggatctcgc
     7981 gcgtcgcctg gttgcccatc tgatgacaga cgttgcagtt ggctttgatc tgattgatcc
     8041 agtgatgctg cgtgcgcatc tcgggcgcga taccgttgcc ggcctctccg gtccccggaa
     8101 attccttctc ggccggaaca tcgatcagcg agtaccagta gttcgccggg tagactcccg
     8161 ccgcagtcct cgcgtcagga gcgacgatgg cggtcaaggc gagcgcgcgc ccgggctgcg
     8221 aacggacagg agaggagtcg atcagcccgt acccgcggac ccagacagtg aaggtcgcct
     8281 tcgccggcag atccgggagc agatagcgcc cgcgatcgtc ggtgacgacg atcttgcgga
     8341 acttcgtc
//
LOCUS       DBNGSH010000651         2458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1765909, whole genome shotgun sequence.
ACCESSION   DBNGSH010000651 DBNGSH010000000
VERSION     DBNGSH010000651.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1765909"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="AC16CY_15070"
     CDS             <1..688
                     /locus_tag="AC16CY_15070"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR3355169.1"
                     /translation="GKPSELDEAEPARVAEAVARMALQYAVVTSVDRDDLADGGASIF
                     AETIRLTRARVPGCRIEVLIPDFKGVESSLRTVLDAGPDVLNHNIETVPRLYRMARSG
                     GRYERSLELLDRARRYRPDIGTKTGIMVGLGEEHDEILAVFDDLRRAGVSILTIGQYL
                     RPSPAHAKMTRYYHPDEFVALKRAALAKGFVHVESGPLVRSSYHAHEQADAWVEQGAG
                     DRGQGGQRSP"
     gene            879..1523
                     /locus_tag="AC16CY_15075"
     CDS             879..1523
                     /locus_tag="AC16CY_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355170.1"
                     /translation="MKSSQARGQYPTTVLIGSEPLIAAWSARTGSGSDVITVALTDLS
                     LAMETITTTKPDVVVIEQAVAASASGAAIMGRLHGERYQRGTEVRLLPPDRAADLMSS
                     GPGELHPQAWLTVLAQALPARPERRAARLQILSEEQALIDGQPVTLIDLSAVGAQVLS
                     PIVLKPRQRVRVVLSAERGSTKAVAIVAWSKLENSTTPGYRAGIAFTEPIPEPV"
     gene            complement(1865..>2458)
                     /locus_tag="AC16CY_15080"
     CDS             complement(1865..>2458)
                     /locus_tag="AC16CY_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR3355171.1"
                     /translation="WNCKAGKCGSCSAEINGKPRLMCMTRLSQVNLLEPVTVEPMRAF
                     PPIKDLVTDVSWNFAVKKGISKFKPRPADAPDGTWRMSQRDIDRVQEFRKCIECFLCQ
                     DVCHVLRDHNKHDEFIGPRFLVYAANLEMNPLDTADRIPELKDEHGIGYCNITKCCTK
                     VCPEHIRITDNAIIPLKERVVDRYYDPLTRLLRIFRS"
BASE COUNT          418 a          825 c          809 g          406 t
ORIGIN      
        1 cggcaagccg tccgagctcg acgaggccga gcccgcacgc gtcgcggagg ccgtggcgcg
       61 gatggcgctt cagtacgccg tcgtgacctc cgtcgatcgg gacgacctgg ccgatggggg
      121 cgcgtccatt ttcgccgaga cgatccggct cacccgcgcg cgcgtgcccg gctgccggat
      181 cgaggtgctc atccccgact tcaagggcgt ggagtcctct ttgcgcacgg tgctggacgc
      241 cggccccgac gtcctgaacc acaacatcga gaccgtgcct cggttgtacc gcatggcccg
      301 gtcgggcggg cgttacgagc gatcactgga actgctggac cgggcgaggc gataccgccc
      361 ggacatcggg accaagaccg gcatcatggt cggcctgggc gaagagcacg acgagatcct
      421 ggcggtcttc gacgacctgc gccgcgccgg cgtgtcgatc ctgaccatcg gccagtacct
      481 gcgtccctct ccggctcacg caaagatgac gcgctactac caccccgacg aattcgtcgc
      541 gctcaagcgc gcggccctcg ccaaaggctt cgtccacgtc gagtccgggc cgctggtgcg
      601 atcctcctac cacgcgcacg aacaggccga cgcgtgggtg gagcaggggg caggggacag
      661 ggggcaaggg gggcagagga gcccgtagag acgtagcccg tagacttggt cgatccccgt
      721 cagatttctg tcgccccaag accttctccc ggtcccgcct tcgggacctc gagcgcgacg
      781 aggtgcaacg ccgcgcaaac cagaccgtcc agccacgcgt cggctgtcgt cgtcaggtgg
      841 cgggtttctt gctccaaatc cgtgaccgaa actcacatat gaaatcaagc caggctcgcg
      901 gacagtaccc gacgaccgtc ctgattggat cggagccgct catcgccgcc tggagtgccc
      961 gcacgggcag cggaagcgac gtcatcacgg tcgccttgac cgacttgtcg cttgcgatgg
     1021 aaacgatcac gacgaccaag cccgatgtgg tcgtgatcga gcaggccgtc gcggccagcg
     1081 cgtcgggagc ggccatcatg ggacggctgc atggcgagcg ctaccagcgc ggcaccgaag
     1141 tccgcctgct gcccccggat cgcgcggccg acctgatgtc gtcgggcccc ggcgaactgc
     1201 acccgcaggc gtggctcacc gtgctggcac aggcccttcc ggcccggccc gagcggcgag
     1261 cggcacgtct gcagattctg tcggaggaac aggcgctgat cgacggacag cccgtcacgc
     1321 tgatcgacct ctccgcggtc ggtgcgcagg tgctgtcgcc gatcgtgctg aagccgaggc
     1381 agcgcgtcag ggtggttctg tcagccgaac gcggatcgac gaaagcggtc gcgatcgtcg
     1441 cctggtcgaa gctggaaaac agcacgacgc ccggctaccg tgccggcatc gccttcacgg
     1501 aacccatccc cgagcccgtg tgacgacgac ggggccaggg cggccccgtg tcgcccgcta
     1561 cgcggcctcg gagtaccgaa ccttcgcctt ccgccgccgg cgaaccttca ggagcgcaaa
     1621 tcccgccacg acgatcatgc tgaggacaag ccacatcatt gagtgcctcc agggtcgagt
     1681 gaaacctcgg agagggcaaa gcatgtaccg caggatcgac ccggccggac gtccccctaa
     1741 cgtgttcagg ctgtgtgaat tagctctcgc gattgccgcc acggagagcg cgcggggccc
     1801 gtcccttcga tcaggtctgt aatcggcttt cagaatcgtc gctacgcggc tgcccgccga
     1861 cgggctatga ccggaagatg cgcaaaagcc tggtaagcgg gtcgtagtac cgatcgacca
     1921 cgcgctcctt gagcggaatg atggcgttgt cggtgatgcg aatgtgctcc ggacacacct
     1981 tggtgcagca cttggtgatg ttgcaatagc cgatgccgtg ctcgtccttg agctcgggaa
     2041 tgcggtccgc ggtgtcgagc gggttcatct cgaggttcgc ggcgtagacg aggaacctgg
     2101 ggccgatgaa ctcgtcgtgc ttgttgtgat cccgcaggac gtggcacacg tcctggcaca
     2161 ggaagcactc gatgcacttg cggaactcct gcacgcgatc gatgtcccgc tgcgacatcc
     2221 gccacgtgcc gtcgggtgcg tccgccgggc gcggcttgaa cttcgagatc cccttcttga
     2281 cggcgaagtt ccacgagacg tcggtcacca ggtccttgat gggcgggaac gcgcgcatcg
     2341 gctccaccgt caccggctcg agcaggttga cctggctgag tcgcgtcatg cacatcagcc
     2401 gcggcttccc gttgatttcg gcggagcacg atccgcactt ccccgccttg cagttcca
//
LOCUS       DBNGSH010000652         2846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3294446, whole genome shotgun sequence.
ACCESSION   DBNGSH010000652 DBNGSH010000000
VERSION     DBNGSH010000652.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3294446"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="AC16CY_15085"
     CDS             <1..371
                     /locus_tag="AC16CY_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355172.1"
                     /translation="RIFKNAFTLEPDRLARAPRPAVLETVADKRVFADAAMTLELHHL
                     RGFLHDEGMLVAYLPRQKLLVQADAFHPRPGAAPLASPSPFTVSLIDNIRRLKLDVER
                     VVHVHGGVDPLATVVRAAGG"
     gene            426..1742
                     /locus_tag="AC16CY_15090"
     CDS             426..1742
                     /locus_tag="AC16CY_15090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3355173.1"
                     /translation="MKPGVRLCTVLVALLTTLHAQVLPQAPPGDVGLSKERLQRLKAA
                     METEVAENRLAGGVGLIARKGKVAYLEPFGAADKEAGTPMRTDTIFRIYSMTKAVTGV
                     AVMVLYEEGRFSLSDPVSRYLPEFATMRVAVERTDPASGKRVLSHSVPAGRPITILDL
                     LRHTSGFNYEGPHDEKGELSYQRLGLAAAGGDDMTLAEFTKRLAQVPLVHEPGTVWDY
                     SFSMDVLGRLVEVVSGRTLDQFFAERIFMPLRMNDTGFYVPDQKWSRLAALYNPAPKG
                     IARADEGAQTAPRRKPALLMGGAGLMSTVIDYARFVQMLLNDGELDGARILGRKTVDL
                     MRSDLLGDLPRAPIRLVVGDGPVVTAQLPPPGYGFGLTFAVSGGPDRTASVGSKGEYN
                     WAGAAGTMFWIDPEEQMVGVLMIQKMFDLSTGAKFKQLAYQAIVAR"
     gene            1751..>2846
                     /locus_tag="AC16CY_15095"
     CDS             1751..>2846
                     /locus_tag="AC16CY_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3355174.1"
                     /translation="MGGRFLLGVLACALCPGCGEKPLEPPQAQDWFVERAKESGIDFV
                     HVNGMSGKFYLAEIMAPGVGLLDFDKDGDLDLYLVQGAGIGGQGAGVRDSGLSDRLYR
                     NDLTVAPDGTRTLRFTDVTRESGLATAGYGMGVAAGDIDNDGWIDIYLTKYAAPNQLF
                     RNNGNGTFTDISRKSGTDQQSWSVSASFADVNRDGWLDLYVANYLQYRLDDVSPCFGA
                     SGSPGYCTPDGYQAVPDRLYLNRRDGTFADVSVKSGVTRTLGPGLGVATADFNGDGWI
                     DIYVANDGQENQLWMNRRDGTFEDAALLSGVALPVTGKAEASMGVDAGDADGDGDEDL
                     FMTELSGEGSNLFVNDGSGSFEDRSTPSGVG"
BASE COUNT          502 a          862 c          995 g          487 t
ORIGIN      
        1 agcgaatctt caagaacgcg ttcacactcg agccggatcg actggctcgc gcgcctcgcc
       61 ccgcggtcct cgagaccgtg gccgacaaga gagtgttcgc ggatgccgcg atgacgctgg
      121 aattgcatca ccttcgcggg ttccttcacg acgaggggat gctggtggcc tatctcccga
      181 ggcagaagct gctcgtacaa gccgacgcgt tccaccctcg acccggcgca gccccgctgg
      241 catcgccctc gccctttacc gtcagcctga tcgacaacat ccggcgcttg aagctggacg
      301 tcgagcgcgt ggtacacgtg cacggcggcg tggatccact cgccacggtc gtgagggcag
      361 cggggggata agcgagactc aaattcacaa cgacacgctg caatctgccg agactggagg
      421 gacgtatgaa gccaggtgtt cgactctgca ctgtgctggt ggcgctgctc accaccctgc
      481 acgcgcaggt gctccctcaa gcgcctcccg gcgacgtcgg cctctcgaag gagcggttgc
      541 agcgcctcaa ggccgcgatg gagacggagg tcgccgagaa ccgcctggcg ggaggcgtcg
      601 ggctgattgc ccgcaagggc aaggtcgcct atctggagcc gttcggcgcg gccgacaagg
      661 aagccggcac gccgatgcgg accgacacga tctttcgcat ctattcgatg accaaggctg
      721 tcacgggtgt ggcagtgatg gtcctgtacg aggaggggcg cttctcgctc tccgatcctg
      781 tctcgcggta cctcccggag ttcgcgacga tgagggtcgc ggtcgagcgc acagaccccg
      841 cgagtggaaa gcgtgtgctc tcgcacagcg tgccggcggg gcgtccgatc acgatcctcg
      901 accttctgcg gcacacgtca ggcttcaact acgaggggcc gcacgacgaa aagggagagc
      961 ttagctacca gaggctggga ctggccgcgg ccggcggcga cgacatgacg ctggccgagt
     1021 tcacgaaacg gctcgcgcaa gtgcctttgg tccatgagcc gggaaccgtc tgggattact
     1081 cgttctcgat ggatgttctg ggccgtctcg tcgaggtggt atcgggccgg actcttgacc
     1141 agttcttcgc cgagcgcatc ttcatgccgc tgcggatgaa cgacacgggc ttctacgtgc
     1201 cggatcagaa atggagccgg ctggcggcgt tgtacaaccc ggcgccgaaa ggcattgcgc
     1261 gtgcggacga aggggcacag acggcgccgc gcagaaagcc cgccctgctc atgggcggcg
     1321 ccgggctcat gtcaaccgtc atcgactacg cgcgattcgt gcagatgctg ttgaacgacg
     1381 gcgagctcga cggggcgcgg atcctcggca gaaagaccgt ggacctgatg cgatcggacc
     1441 tcctcggtga tctgcccaga gcgccgatca ggcttgtcgt cggagacggc cccgtggtga
     1501 ccgcgcagct tccgcccccc ggttacgggt tcgggctgac attcgccgtg agcggcggac
     1561 cagatcggac agcgtcggtc ggttcgaagg gtgaatacaa ctgggccggc gctgcgggca
     1621 cgatgttctg gatcgatccg gaagagcaga tggtcggcgt gctcatgatc cagaagatgt
     1681 tcgatctgtc gacgggggcc aagttcaagc agctcgcgta tcaggcgatc gtggcgcggt
     1741 gaaggccgcg atgggcggtc ggttcctgtt gggcgtgctc gcgtgcgcac tgtgtcccgg
     1801 ctgcggcgag aagccactgg aaccgcctca ggcgcaggat tggttcgtcg agcgcgcgaa
     1861 agagagcggc atcgacttcg ttcacgtgaa cgggatgtcc ggcaagttct atctggccga
     1921 gatcatggcg ccgggcgtcg ggttgctcga cttcgacaag gacggcgatc tcgacctgta
     1981 tctggtgcag ggggcaggga tcggggggca gggggcaggg gttcgcgatt cggggttgtc
     2041 ggatcgtctc tatcggaacg atctcactgt cgcgcccgat gggacgcgga cgctgcgctt
     2101 caccgacgtc acgcgcgaga gcggcctcgc gacggcagga tacgggatgg gcgttgcggc
     2161 aggcgacatc gacaacgacg gctggattga catctacctg acgaagtacg cggcgccgaa
     2221 tcagctgttt cgcaacaacg gcaacggtac gttcaccgac atctccagga agagcggaac
     2281 ggaccagcag tcgtggagcg tgtcggcctc gtttgccgac gtcaaccgcg acggctggct
     2341 ggatctgtat gtcgccaact acctgcagta caggctcgat gacgtgagtc cgtgcttcgg
     2401 ggcgtccggg tcgccaggct attgcacgcc ggacggctat caggccgtgc cggaccggtt
     2461 gtacctcaat cgccgcgacg ggaccttcgc cgacgtgagc gtcaagtcgg gtgtcacccg
     2521 cacactcggg ccggggctcg gtgtcgccac ggccgatttc aacggcgacg gatggatcga
     2581 catctacgtg gccaacgacg ggcaggagaa tcagctgtgg atgaaccggc gtgacggtac
     2641 cttcgaggac gcggcgctgc tgtccggggt ggctctcccg gtgaccggca aggccgaggc
     2701 cagcatgggc gtggacgctg gcgacgcgga cggcgacggg gacgaagatc tgttcatgac
     2761 ggagctgagc ggcgaaggga gcaacctgtt cgtcaacgac ggatccggat ccttcgagga
     2821 ccgcagcact ccctccggcg tcggcc
//
LOCUS       DBNGSH010000653         2919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3633739, whole genome shotgun sequence.
ACCESSION   DBNGSH010000653 DBNGSH010000000
VERSION     DBNGSH010000653.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3633739"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="AC16CY_15100"
     CDS             complement(<1..877)
                     /locus_tag="AC16CY_15100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3355175.1"
                     /translation="MRRLVVVGNGMAGMACVEQILKYAPSFRITVFGDETHVNYNRIM
                     LSSVLAGEKSLDDIVLNGLDWYRRHEIDLHVGVRIVDVDPAAKTVTGDDGRVTPYDTL
                     LLATGSSAWMPPIKGLDKEGVLTFRTLDDTRALLARAGPDTSAVVIGGGLLGLEAARG
                     LEVQGCRVTVVHLMATLMERQLDPAGSRFLMAKMKELGIHVTLGRSTTAITGDRHADG
                     VEFSDGERLDANLVVVAAGIRPNVELARKAGLAVKRGIVVNDFMETSDPDIFAVGECV
                     EHRGVCYGLVAPLFEQ"
     gene            complement(874..>2919)
                     /locus_tag="AC16CY_15105"
     CDS             complement(874..>2919)
                     /locus_tag="AC16CY_15105"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR3355176.1"
                     /translation="SEHHTIAADNRALHPLLRKQGALARVSWDEALTTMAERFREIQA
                     RHGSDAVGVISTGQLVTEEFYALGKLAQLGIGTRNIDGNTTLCMATAVSGYKLSFGSD
                     GPPGAYEDLERADVILLIGANIAENHPILCWRLQANPGATLIVVDPRVTKTAMLADLH
                     LPVKPRSDLALVNGLIHFVIEHDLIDRAYIERHTTGFDALRESVRPYTPERVAELTGI
                     SAELVCRTAWTYARARAGFIAWTMGVNHSTKGTETVNAINNLALITGNIGRAGAAPFS
                     ITGQCNAMGTREAGFASSLPGYRRFESAEDREDLARLWGLPVERIPNARGLAYPDIIE
                     AALDRRIRGLWIIATNPVVSFPNVAALRQALEGLDFLVVQDGYHPTPTSEYAHLMLPA
                     AMWGEKEGTYTNSERRVSKVNRAVAPPGDARADFDIFLDLARVLGVKDEIFPGWAAPS
                     DAFEEWKRVSAGRLCDYSGMTYKAIERHGGLQWPLPAGAAHDGEARRLYADGCFQTED
                     RRARLIAGTWEPFPEQPSREYPFVLNTGRTVEHWHTRTKTSNVPILERLSPNAWVEMN
                     PADARALRLKPQRRVDIVSRRGRIQNVELRVTETVAPGQLFVPFHYFEANANQVTQSA
                     FDPISREPNYKQSAVRVEAASTSNSQSPTPKRTPSRLGIGNWKLGVDRRADREGGAR"
BASE COUNT          455 a          984 c          989 g          491 t
ORIGIN      
        1 cctgctcgaa gagcggcgcc accagcccgt agcacacgcc gcgatgctcg acgcattcgc
       61 ccacggcgaa gatgtcggga tcggacgtct ccatgaagtc gttcacgacg atgccgcgct
      121 tgacggcgag gcccgccttg cgcgcgagct ccacgttcgg ccgtatgccg gcagcgacca
      181 caacgagatt ggcgtcgagg cgctcgccgt cggagaactc gacgccgtcc gcgtgccggt
      241 cgccggtgat ggcggtcgtg ctgcgaccca gggtgacgtg aatgccgagc tccttcatct
      301 tggccatcag gaaccggctg ccggccggat cgagctggcg ctccatcagc gtcgccatca
      361 gatggacgac ggtgaccctg cagccctgca cctcgaggcc gcgcgccgcc tcgagcccga
      421 gcagcccccc gccgatgacc acggcgctgg tgtccggacc ggcgcgcgcg agcagcgcgc
      481 gcgtgtcgtc gagcgtgcgg aaagtcagca cgccctcctt gtcgagcccc ttgatgggcg
      541 gcatccacgc cgagctgcca gtcgccagca gaagcgtgtc gtacggcgtg acccgcccgt
      601 cgtctcccgt aacggtcttc gccgcgggat cgacgtcgac gatgcggacg cccacgtgga
      661 ggtcgatctc gtggcgccgg taccagtcga gcccgttgag cacgatgtcg tcgaggctct
      721 tctcgccggc cagcaccgac gagagcatga tgcggttgta gttgacgtgc gtctcgtcgc
      781 cgaacacggt gatgcggaac gacggtgcgt acttgagaat ctgctccacg cacgccattc
      841 cggccatgcc gttgccgacg acgacgagcc ttctcatcgc gcacctccct cacggtcggc
      901 gcgccggtca actcccaact tccaattccc aatccccaac cgggagggtg tccttttggg
      961 agttggagat tgggagttgg aagttgaggc ggcctcgact ctcacggccg actgcttgta
     1021 attcggctcg cgggaaatcg ggtcgaaggc gctctgcgtc acctggttcg cgttggcctc
     1081 gaagtagtgg aacgggacga agagctggcc cggagccacg gtctcggtca cgcgcagctc
     1141 gacattctgg atacggcctc tccgcgagac gatatccacg cgccgctgag gcttcaggcg
     1201 cagcgcgcga gcgtcggccg ggttcatctc cacccaggcg ttgggcgaca gccgttcgag
     1261 gatcgggacg ttcgaggtct tcgtgcgcgt gtgccagtgc tcgacggtgc gtcccgtgtt
     1321 caggacgaac ggatactcgc gggacggctg ctcgggaaac ggctcccacg ttccggcaat
     1381 cagcctggcg cggcgatcct cggtctggaa gcagccgtcg gcgtacaagc ggcgcgcttc
     1441 cccatcgtgc gccgcgccgg cagggagcgg ccactggagg ccgccgtgcc gctcgatggc
     1501 cttgtaggtc attccggagt agtcgcagag ccgtcctgcc gaaacgcgct tccactcctc
     1561 gaacgcgtcg gagggcgccg cccagccggg aaagatctcg tctttcacgc cgagcacgcg
     1621 ggcgagatcc aggaagatgt cgaagtctgc gcgcgcgtct ccgggcggtg caaccgcccg
     1681 attgaccttg ctcacacggc gctcggaatt ggtgtaggtg ccctccttct cgccccacat
     1741 cgccgccggc agcatcaggt gtgcgtattc cgacgtgggc gtcgggtgat agccgtcctg
     1801 gaccaccagg aagtcgagcc cctcgagcgc ctgccggagc gcggcgacgt tcgggaacga
     1861 cacgacgggg ttggtcgcga tgatccacaa accacgaatg cggcgatcga gcgcggcctc
     1921 gatgatgtcc ggataggcga gaccacgcgc gttcggaatg cgctccaccg gcagacccca
     1981 gagccgcgcg aggtcctcgc ggtcctcggc gctctcgaac cggcgatagc caggcaggct
     2041 cgacgcgaac ccggcctcac gcgtgcccat cgcgttgcac tgcccggtga tcgagaacgg
     2101 cgccgctccg gcgcggccga tgttgccagt gatgagcgcg aggttgttga tcgcgttgac
     2161 cgtctcggtg cccttcgtgc tgtggttgac ccccatcgtc cacgcgatga agccagcgcg
     2221 cgcgcgggcg tacgtccacg cggtgcggca cacgagctcg gctgagatcc cggtcagctc
     2281 ggccacccgt tccggcgtat acggccgaac cgattcccgg agcgcgtcga accccgtggt
     2341 gtggcgttcg atgtaggcgc gatcgatcag atcgtgctcg atgacgaagt ggatcaggcc
     2401 gttgacgagc gcgagatcgg accgcggctt gaccggcaga tggaggtccg caagcatcgc
     2461 cgttttcgtc acgcgcggat ccaccacgat gagcgtggcg ccggggttgg cctgcaggcg
     2521 ccagcagagg atcgggtggt tctcggcgat gttggccccg atgaggagaa tcacgtccgc
     2581 gcgctccagg tcctcgtagg cgcccggcgg tccatcgctg ccaaacgaca gcttgtaccc
     2641 cgacacggcg gtcgccatgc agagcgtcgt gttgccgtcg atgttgcgcg tcccgatccc
     2701 gagctgtgcg agcttgccga gcgcgtagaa ctcctcggtc acgagctgac cagtgctgat
     2761 cacgccgaca gcgtcgctcc catgccgggc ctgaatctcc cggaagcgct ccgccatcgt
     2821 cgtcagcgcc tcgtcccagc tcacgcgcgc cagggcgccc tgcttgcgga gcagcgggtg
     2881 gagggcgcga ttgtcggcgg cgatcgtgtg gtgctcgga
//
LOCUS       DBNGSH010000654         8085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3294560, whole genome shotgun sequence.
ACCESSION   DBNGSH010000654 DBNGSH010000000
VERSION     DBNGSH010000654.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3294560"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="AC16CY_15110"
     CDS             complement(<1..885)
                     /locus_tag="AC16CY_15110"
                     /inference="COORDINATES: protein motif:HMM:NF045169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR3355177.1"
                     /translation="MTIRPGFLGFRRRDGRVGTRNHVLVVPTVICSSVVAERIAAATP
                     PLGVALPHLAGCGQLGPDMRVTHETLAAYCAHPNVGAVVVVALGCEQVLAQSLADVAR
                     RHHKPVDIVGIQAEGGSLKATEKGTRIAQALAGTLAGEERVWCDISELVLSLKCGGSD
                     YTSGLASNPTLGRVTDRLVDLGGSAVLGEIAEIMGAEHVLASRAKNPATAADLIQVIT
                     RVETEARALGLDIRGTQPSPGNIRGGLTTIEEKSLGATHKGGERAPLEDVVGYAAPIQ
                     RKGLTVMDTPGLDVESVTG"
     gene            complement(882..1268)
                     /locus_tag="AC16CY_15115"
     CDS             complement(882..1268)
                     /locus_tag="AC16CY_15115"
                     /inference="COORDINATES: protein motif:HMM:NF020250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR3355178.1"
                     /translation="MKAVLVISARDNVATALRALAPGQRIDTGGTVVIVAEPIARGHK
                     VALQPIASGGPVIKYGSAIGTASADIPAGAHVHTHNVASRRGRGDLGVPATGLEARLA
                     EPPDDPPGRGSDIPGAPTTMSKESSS"
     gene            complement(1265..2032)
                     /locus_tag="AC16CY_15120"
     CDS             complement(1265..2032)
                     /locus_tag="AC16CY_15120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3355179.1"
                     /translation="MNAFDLSGRAALVVGGTSGIGRTLALGLADAGADVVATGRRAAL
                     VEEVAAEIERKGRRTARVPADVNDVTALERVREACLATFGRIDILVCAAGITRRVPTL
                     DMAEADWQRIIDTNLTGTLRSCQVFGRDMVARGSGRVITIASLSSFLGLFEVLAYGAS
                     KSAVAGLTRALAVEWAPHGVTVNAILPGVFKTDLNRALLDSPRGQELLMRTPMRRFGR
                     VEELVGAAVYLASDAASFVTGHLLAVDGGFLASGVNQ"
     gene            complement(2089..2880)
                     /locus_tag="AC16CY_15125"
     CDS             complement(2089..2880)
                     /locus_tag="AC16CY_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3355180.1"
                     /translation="MGSTAVRRKEERASEGSTEQVVSHVRQLIEQGALRPGDRLPAER
                     DLAAHVGVSRPTVRAGLRALAAIGVVQSRHGSGTYIPDGPPLLGTDALSFLAALHGFT
                     RDEMYEARRILEVGAAGLAAERATPDQIATLAEEVASLFAAMADPQVFLVHDINFHRA
                     VATASGNPIVASLVEMVSALYYERRRDTATRASNRDMRDAAEMHRRIYQAIRAHDADQ
                     ARRAMETHLMQASAHQAKEADGSDAAPRAAAPKAKGRRQGGRPTV"
     gene            complement(2927..6508)
                     /locus_tag="AC16CY_15130"
     CDS             complement(2927..6508)
                     /locus_tag="AC16CY_15130"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3355181.1"
                     /translation="MRARTTWAALIVAMMAVAVPGPSAQEFRGTIEGTVKDTTGGVLP
                     GVTVTVTNTATSVAQDTVTDASGRYRVLYLNPGNYSVSAEIAGFKKFVHLDADVRVGD
                     VVRVDVVLEAGGVSETVSVTAERSLLNTSSGISGTTIDSKQITELPLGDGTAYMLTRL
                     APGILDTSDLHFARPADNANLGGIVANGALGGNEFSIDGSPNMSNQRGVGFSPPSDAI
                     SQFKVQTNAFDAQTGHTAGAVVNLAVASGTNAVRFAAGYFNRSDKRSETPLLTERNNG
                     SKPTREYNRYTGTLSGPVVRNRTFFMASFEHLRDVQPEPATYTVPTERMRNGDFGEFS
                     TLVYDPLTATSAGVRSPFTDNRIPTGRIDPVAAAYTALYPLPNRPGTVSNYFTNQLRP
                     YDYNAGMGRVDHNFTDANRMYGTVYWNKRQEDRYNWAQDAANATGGGIINGFAVTKGF
                     DYRTNTGVTSGYTSTLSNSTLLDVRGNWSRFGEYRDPAEEFDPASLGFASSAVQAMGD
                     YRYLPFITIGAFSQTNDSSTIASLGSRRSDWREGFDRPLNTASAAATLTRVLGQHTAR
                     AGYDYRHQNWAITDGGYPGGRFRFDGSYTRANNSAATNDRAQSFAQFLLGLPTATTGA
                     VAAASTQTSQFEMTSPGEFTQIYHHFFVQDDWQVSPRLTLNLGMRFEINSGMREVLDR
                     NLAGFDTITSNPIEAQAAANYARNPIPEIPVSAFQVNGGLLFADGPVNDTATKWLPRA
                     AAAFMLDDRTVLRGGVGLFSYDYFFENINQSGFSQATPVLTTSNNGVSFTGVTLSNPL
                     PSGELIQPPGSSLGLQTSLGQGLGTMYDSNRVTPYYTRWEMSLQRDFGSSVVGSVIYV
                     GSRGSDLPVVRAINNIPMNYLSTSRSRDAANETYLTTNVPSPFTGLLPGSTINGATVQ
                     RQQLLRPFPHFIAISREEYTGSDSYSAVSFQVEKRFRGVSSVTAQYTRSSLRDKLNFL
                     NPQDGELEDRVAPNDRPHRFSTAAIMRLPFGHEQKWGNDWNDLLDAVAGGWQLSTTYQ
                     YQSGAPLAFTGNVYFDASCDPGNLKSTIGQQVSGGIGGLDVPAWDVSCFYFHDAPVQT
                     GGVDDPAKQQADQRIAVATTNNVRYFPSTMTGMRTDDVHLLDIGLSKNFRLPRSMTFQ
                     IRFEVINALNYTVLWNPDLNPRNSTFGLVNQDRNNPRDIQIGARLTF"
     gene            complement(6626..8008)
                     /locus_tag="AC16CY_15135"
     CDS             complement(6626..8008)
                     /locus_tag="AC16CY_15135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019113.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIR3355182.1"
                     /translation="MHKGQPGVVTSAAAAALALVVAAGAQAPAPPQGAGSSDPSFSQP
                     SIPPGIAYTVPAEAEIVQSLGRIREHFVRSTPYRLVDTATGQPLRDVTTPTQTAGIDL
                     GPGEFNDWTYSMGVVLAGMLHATDVTGDRSFESYTRRNFDFIFEHLEFFRRQAAAFGP
                     QPYGYRRLMEMRELDDCGAIGAALIKAYLRWKDPKYRPVIDLVNDFVSRQMTRMPDRT
                     IARARPHPVSIWVDDLYMSVPFLAQMGALTGQRQYFDDAATQVVQYAARLMNPNTGLF
                     DHSWFAHEPADPKFYWGRGGGWALMATAELLSVMPEQHPRRAQVLDVFRRGVQGVAGV
                     QSGSGFWHQLLDREDSYLESSATAMFTFAIARGVNRGWIQPVYAPIAQTGWRALETRI
                     RPDGTIDGIVVATTAAYDAVYYYNRPTDPRAMQGFGAALMAGAEVLEMIRRFDIQKTL
                     NTYHYRPKKG"
BASE COUNT         1191 a         2728 c         2829 g         1337 t
ORIGIN      
        1 gcccgtcacc gactcgacgt cgaggccggg cgtgtccatg acggtcagcc ccttgcgctg
       61 gatgggcgcg gcatagccga ccacgtcctc gagcggcgcc cgctcgcccc ccttgtgggt
      121 cgcgcccagc gacttttcct cgatggtcgt cagaccgccg cggatgttgc cgggcgaggg
      181 ctgcgtcccg cggatgtcca gcccgagcgc ccgcgcctcg gtctccaccc gcgtgatcac
      241 ctgaatcagg tcggcagccg tcgccgggtt cttcgcgcgc gacgccagca cgtgctcggc
      301 acccatgatc tccgcgatct cgccgagcac cgcgctcccc ccgagatcga cgagccgatc
      361 cgtcacgcgc ccgagcgtgg gattggacgc gagccccgag gtgtagtcgg agccgccgca
      421 cttgagcgag agcacgagct cggagatgtc gcaccacacg cgctcctcgc cggcgagcgt
      481 gccggcaagg gcctgcgcga tgcgcgtccc cttctcggtc gccttcagcg agcccccctc
      541 ggcctgtatc cccacgatgt ccaccggctt gtgatgacgg cgggcgacgt cggccagcga
      601 ctgggccagc acctgctcgc acccgagcgc caccacgacg acggccccca cgttggggtg
      661 cgcgcagtac gcggccagcg tctcgtgcgt cacgcgcatg tcggggccga gctgcccgca
      721 gcccgccagg tgcggcaatg ccaccccgag cggcggcgtc gccgccgcga tccgttcggc
      781 gactaccgac gagcagatca cggtcgggac gacgagcacg tgattgcgcg tgcccacgcg
      841 gccgtcgcgc cgccggaatc cgaggaagcc gggccggatg gtcacgagga cgactccttc
      901 gacatggtgg ttggagcgcc ggggatgtcg cttccgcggc ccggcggatc gtcgggcggc
      961 tcggcaagcc tcgcttccag acccgtggcg ggcacgccga ggtcgccgcg tccgcggcgg
     1021 ctcgcgacgt tgtgcgtgtg cacgtgggcg ccggcgggaa tgtcggcgct cgccgtgccg
     1081 atggcgctgc cgtacttgat caccgggccg cccgaggcaa tcggctggag cgcgaccttg
     1141 tggccgcgcg cgatcggctc ggcgacgatc acgacggtgc cgcccgtgtc gatgcgctgc
     1201 ccgggcgcga gcgcgcggag cgccgtcgcc acgttgtcgc gcgcgctgat cacgaggacg
     1261 gctttcactg gttcaccccg ctcgccagga acccgccgtc cacggcgagc aggtggccgg
     1321 tgacgaagga tgccgcatcc gaggcgaggt acaccgccgc gcccaccagc tcttcgaccc
     1381 gcccgaaccg gcgcatgggc gtccgcatca gcagttcctg accgcgcggc gaatcgagca
     1441 gcgcccgatt gaggtcggtc ttgaacacgc ccggcaggat ggcgttgacg gtgacgccgt
     1501 ggggcgccca ctccacggca agcgcccggg tcaggccggc cacggcggac ttgctggccc
     1561 cgtacgcaag gacttcgaac agcccgagga acgaggagag cgacgcgatg gtgatgacgc
     1621 gaccggaccc gcgcgccacc atgtcgcgtc cgaagacctg gcaggacctc aacgtgccgg
     1681 tgaggttcgt gtcgatgatg cgctgccaat cggcttcggc catgtccagc gtcggcacgc
     1741 gcctggtaat ccccgcggcg cacaccagaa tgtcgattcg gccgaacgtg gcgaggcagg
     1801 cctcccggac gcgctcgagc gccgtgacgt cgttcacgtc ggcaggcacg cgcgccgttc
     1861 gtcggccctt ccgctcgatc tcggccgcca cctcctcgac gagcgcggcg cgccggccgg
     1921 tcgcgacgac gtcggcgccc gcgtcggcga gcccgagggc aagcgtccgg ccgatgccgc
     1981 tcgtaccgcc gacgacgagg gctgcgcggc cggagagatc gaacgcgttc atgtactcca
     2041 catcggaaac gacctggcga atgggatcaa cacacgcgag tcctggggtt agaccgttgg
     2101 acggccgcct tgccgacggc ctttcgcttt gggcgccgcg gccctgggag cggcgtcgga
     2161 gccgtcggct tccttcgcct gatgggcgct ggcctgcatc aggtgcgttt ccatggcccg
     2221 ccgcgcctgg tccgcatcgt gggcgcgaat ggcctggtag atccggcggt gcatttccgc
     2281 cgcgtcgcgc atgtcgcggt tgctcgcacg cgtggccgtg tcgcgacggc gctcgtagta
     2341 cagcgcggac accatttcga cgagcgacgc cacgatcgga ttgcccgacg ccgtcgccac
     2401 ggcgcggtgg aagttgatgt cgtggaccag aaacacctgc ggatccgcca tcgcggcgaa
     2461 caggctcgcc acctcctcgg ccagcgtcgc aatctggtcc ggcgtcgccc gctcggccgc
     2521 caggccggcg gcgccgacct ccaggatgcg gcgggcctcg tacatctcgt cgcgcgtgaa
     2581 tccgtggagc gcggcaagga agctcagcgc atcggtgccg agcagcggcg gtccgtcggg
     2641 gatgtaggta cccgacccgt gccgcgactg cacgacgccg atcgcggcca gcgcgcggag
     2701 ccccgcacgc acggtcggac ggctgacgcc gacgtgcgcc gcgagatcgc gctcggcggg
     2761 caggcggtcg cccggccgca gcgcgccctg ctcgatgagc tggcgcacgt gcgaaacgac
     2821 ctgctccgtg cttccctcgc tcgcgcgctc ctctttccgc cggacggctg tgctccccat
     2881 actggtctag ccaattatag actcggacct ccgagcccct cgcgcgtcag aacgtgagcc
     2941 gcgctccgat ctggatgtcg cgaggattgt tccggtcctg gttgaccagc ccgaacgtcg
     3001 agttgcgcgg attcaggtcc gggttccaga gcacggtgta gttcaacgcg ttgatgacct
     3061 cgaaccggat ctggaacgtc atcgagcgcg gcagccggaa gttcttcgag agcccgatgt
     3121 cgagcaggtg cacgtcgtcg gtccgcatgc ccgtcatcgt cgaggggaag tacctgacgt
     3181 tgttcgtcgt cgcgacggcg atccgctggt cggcctgctg ctttgccgga tcgtccacgc
     3241 cgccggtttg caccggcgcg tcgtggaaat agaagcagga cacgtcccac gccggtacat
     3301 ccaggccgcc gatgccgccc gacacctgct ggccgatggt ggacttgagg ttgcccgggt
     3361 cgcaggaggc gtcgaagtag acgttgcccg tgaacgcgag gggcgcgccg ctctgatact
     3421 ggtaggtggt gctcagctgc cagcctccgg ccaccgcgtc gagcagatcg ttccagtcgt
     3481 tgccccattt ctgctcgtgt ccgaacggca ggcgcatgat ggcggccgtc gagaagcggt
     3541 gcggccggtc gttgggcgcc acgcgatcct cgagctcgcc gtcctgggga ttgaggaagt
     3601 tcagcttgtc gcgaagggat gaccgcgtgt actgcgcggt caccgagctg acgccccgga
     3661 agcgcttctc gacctgaaac gacacggcgc tgtagctgtc cgaccccgtg tactcctcgc
     3721 gcgagatcgc gatgaagtgc gggaagggcc gcagcagctg ctggcgctgg accgtggcgc
     3781 cgttgatcgt gctgcccggc agcagcccgg tgaaggggct cgggacgttg gtcgtcaggt
     3841 acgtctcgtt cgccgcgtcg cggctgcgcg aggtcgagag gtaattcatc gggatgttgt
     3901 tgatcgcgcg caccacggga aggtccgatc cgcgcgagcc gacgtagatg accgatccca
     3961 cgacgctgct gccgaagtcg cgctgcaggc tcatctccca gcgcgtgtag tacggcgtca
     4021 cgcgattgct gtcgtacatc gtgccgagcc cctggccaag ggacgtctgg aggcccaggc
     4081 tcgagcccgg cggctgaatc aattcgccgc tcggcagcgg gttgctgagc gtcacccccg
     4141 tgaagctcac gccgttgttg ctcgtcgtca gcaccggcgt cgcctgcgag aaaccggact
     4201 gattgatgtt ctcgaagaag tagtcgtacg agaagaggcc gaccccccct cgcagcacgg
     4261 tgcgatcgtc gagcatgaac gccgcggccg cgcgcggcag ccacttggtc gccgtgtcgt
     4321 tgaccgggcc gtcggcgaag agcagcccgc cgttcacctg aaaggccgac accgggatct
     4381 cggggatcgg gttgcgggcg tagttggcgg ccgcctgcgc ctcgatcgga ttcgacgtga
     4441 tcgtgtcgaa gcccgcgagg ttgcggtcca gcacttcgcg catgccggag ttgatctcga
     4501 agcgcatgcc gaggttgagc gtgaggcggg ggctgacctg ccagtcgtcc tgcacgaaga
     4561 agtggtgata gatctgcgtg aactcacccg gtgaggtcat ctcgaactgg ctcgtctgcg
     4621 tcgaggccgc ggccacggcg cccgtcgtgg cggtggggag cccgagcagg aattgcgcga
     4681 acgactgcgc gcggtcgttg gttgccgcgg agttgttcgc ccgggtgtag gagccgtcga
     4741 agcggaagcg ccctccgggg tacccgccgt cggtgatggc ccagttctgg tgccggtagt
     4801 cgtatccggc gcgagccgtg tgctgcccca ggacccgtgt gagcgtcgcc gcggcggagg
     4861 ccgtgttcag cggccggtcg aacccctcgc gccagtccga gcgtctcgac ccgagcgacg
     4921 cgatcgtcga gctgtcgttg gtctggctga aggcgccgat cgtgatgaag ggcagatagc
     4981 gatagtcgcc catcgcctgg accgcggagc tggcaaaacc gagcgacgcc gggtcgaact
     5041 cttccgccgg gtcgcggtat tcgccgaagc gcgaccagtt tccacggacg tcgagcagcg
     5101 tcgagttgct cagcgtggac gtgtagccgc tggtcacgcc ggtgttggtc cggtagtcga
     5161 agcccttggt gacggcgaag ccgttgatga tgccgccccc ggtggcatta gcggcgtcct
     5221 gggcccagtt gtaccgatcc tcctggcgct tgttccagta caccgtgccg tacatccggt
     5281 tggcgtcggt gaaattgtga tcgacccgtc ccatgcccgc gttgtagtcg tagggacgga
     5341 gctggttggt gaagtagttg ctcacggtcc ccggccggtt gggcagcgga tagagcgcgg
     5401 tgtaggcggc cgcgacggga tcgatgcgcc cggtcggaat ccggttgtcg gtgaacggcg
     5461 agcgaacgcc ggccgaggtc gccgtcaacg gatcgtagac gagcgtggag aactctccga
     5521 agtcgccgtt ccgcatccgc tcggtcggca ccgtgtaggt ggccggctcc ggctgcacgt
     5581 cgcgaaggtg ctcgaacgac gccatgaaga aggtcctgtt gcgcacgacg ggaccgctca
     5641 gcgtgccggt gtagcggttg tactcccgcg tgggcttgga cccgttgttt cgctcggtga
     5701 gcagcggcgt ctcggagcgt ttgtcgctgc gattgaaata gcccgcggca aagcgcacgg
     5761 cgttggtgcc gctcgcgacc gcgaggttga cgacggcccc cgcggtgtgg ccagtctgcg
     5821 cgtcgaacgc gttggtctgg accttgaact gggaaatcgc gtccgagggc ggcgagaagc
     5881 cgacgccgcg ctggttggac atgttcggcg acccgtcgat cgagaactcg ttgcccccca
     5941 gtgcgccgtt ggcgacgatc cccccgaggt tcgcgttgtc tgccggccgc gcgaagtgca
     6001 ggtccgacgt gtcgagaatc cccggcgcga ggcgcgtgag catgtaggcc gtgccgtcgc
     6061 cgagcggcag ctcggtgatc tgcttgctgt cgatcgtcgt gccgctgatc ccggagctgg
     6121 tgttgagcaa cgagcgctcg gccgtcaccg acaccgtctc gctgaccccg ccagcctcga
     6181 gcacgacgtc cacgcggacc acgtcgccca cgcggacgtc ggcatcgagg tggacgaact
     6241 tcttaaagcc cgcgatctcg gcgctgacgc tgtagttgcc gggattcagg tacaggacac
     6301 ggtaccggcc gctcgcgtcg gtcaccgtgt cctgcgcgac ggacgtcgcc gtgttcgtca
     6361 cggtcacggt cacgccgggc aggacgccgc cggtcgtgtc cttcaccgta ccttcgatgg
     6421 ttccccggaa ctcctgggcg gacggccccg gaacggcgac ggccatcatc gccacgatca
     6481 gtgcggccca cgtcgtacgc gcgcgcatct ttcggcctcc tgcggaactg gacggcacac
     6541 gtctcgtgcc gaacggcaag aggtaatacc actttgtgta tcgcgtgtca acaggacaag
     6601 tcagatgcga tgcgccccgg ccgcgtcacc ccttcttcgg ccggtagtgg tacgtgttga
     6661 gggtcttctg aatgtcgaac cggcggatca tctcgagcac ctcggcgccc gccatcagcg
     6721 cggcgccgaa cccctgcatc gcccgcggat cggtgggccg gttgtagtag tagaccgcgt
     6781 cgtacgcggc cgtcgttgcg acgacgatgc cgtcgatcgt gccgtcgggt cgaatgcgcg
     6841 tctcgagcgc gcgccagccc gtctgcgcga tcggcgcata cacgggctgt atccatccgc
     6901 ggttgacgcc cctggcgatg gcgaaggtga acatcgccgt tgcggaggac tccaggtagc
     6961 tgtcttcgcg gtcgaggagc tggtgccaga atccgctgcc gctctgcacg ccggcgacgc
     7021 cctgcacgcc gcggcggaac acgtcgagca cctgcgcgcg ccgcggatgc tgctccggca
     7081 tgacgctgag cagttccgcc gtcgccatca acgcccaccc tccgccgcgt ccccaataga
     7141 actttggatc cgcaggttcg tgcgcgaacc aggagtgatc gaacagcccc gtgttcgggt
     7201 tcatcaggcg cgcggcgtac tgcacgacct gtgtcgccgc gtcgtcgaag tactggcgct
     7261 ggcccgtcag cgcgcccatc tgcgcgagaa acgggacgct catgtacaag tcgtccaccc
     7321 atatcgagac cgggtgcgga cgcgcgcgcg cgatcgtgcg atcgggcatc cgcgtcatct
     7381 gtcgcgacac gaagtcgttc acgaggtcga tgaccggccg gtatttcggg tccttccacc
     7441 gcagataggc cttgatgagc gcggcgccga tggccccgca gtcgtccagt tcccgcatct
     7501 ccatcaggcg gcggtacccg tagggctgcg ggccgaatgc ggccgcctgc cggcggaaga
     7561 actcgagatg ctcgaagatg aagtcgaagt tcctgcgggt gtagctctcg aacgagcgat
     7621 cgccggtcac gtcggtggcg tgcagcatgc cggcgagcac gacacccatc gagtacgtcc
     7681 agtcgttgaa ctcgccggga cccagatcga tgccggccgt ctgcgtcggc gtggtcacgt
     7741 cgcggagcgg ctgcccggtc gccgtgtcga cgagacggta cggcgtggac cgcacgaagt
     7801 gctcccggat ccggccgagc gactgcacga tctcggcctc ggccggcacg gtgtacgcga
     7861 tgccgggcgg aatcgagggt tgagagaacg acgggtcgct gctgcctgcg ccctgcggcg
     7921 gcgccggcgc ctgggctccg gcggcaacga ccagcgcgag cgccgcggcg gcggcagacg
     7981 tcaccacgcc gggttggcct ttgtgcatcg aacgctcccc ttgcgcgcca tgacggtcgc
     8041 gcggtattgg tatagccact ttgcgatgag aacgcaagcc gggtc
//
LOCUS       DBNGSH010000655         3674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_68288, whole genome shotgun sequence.
ACCESSION   DBNGSH010000655 DBNGSH010000000
VERSION     DBNGSH010000655.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_68288"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="AC16CY_15140"
     CDS             complement(<1..1322)
                     /locus_tag="AC16CY_15140"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3355183.1"
                     /translation="MSRVDRSRLPEVGADPAFTFPAIVRHTLANGMNVRTVEHHSVPV
                     ITFALQVDGGSGADPVDSEGLAALVADMADEGSGSRSAIEMSDALARIGGEYDVDVGG
                     DSTLFTLTTLTRFASRGASLLADMVARPSLRDSDFTRVRQLRLDRLRQLKDLASALAE
                     YRFLKLVYGTHPYGHLPIGGDASLRRISLDEVAAFHRDTFQPPRTTLVVCGAMSHEGL
                     LRCAEDGFGGWAPSRGGAVAAAAAAEPPRAPLARLAVVAREGAAQSELRIGHLTTGRA
                     TPDYPALLVMNAVLGGQFVSRVNLKLREEKGYTYGARTGFDWRRGASPFVLQTSVHTS
                     ATADAITLSLAEIDAIRGLRAPSETELALAKASLTRGYPRGFEAAQQVARSVAQIALY
                     GLPDTYFSEFVPKVNAVTGADVVDAAARHLDSSRLTTLVVGDHASIADT"
     gene            complement(1319..2638)
                     /locus_tag="AC16CY_15145"
     CDS             complement(1319..2638)
                     /locus_tag="AC16CY_15145"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3355184.1"
                     /translation="MTSAAIDLPFSMRTLGNGLDVIVHEDHHVPIVAVNVWYHVGSKN
                     ERPGRTGFAHLFEHLMFEGSEHHNSGYFPPLQQAGALLNGSTNTDRTNYWEVVPTSAL
                     DRALWMESDRMGYLLPALTRERFENQRDVVLNERRQSYENRPYGMVMMAMSAALYPPD
                     HPYSWMTIGSADDIRAMQLDEVQEFFRTYYHPSNASLVLAGDIDAARGFELAEAYFGD
                     LPPGAAPAPVSPSADLSSESRLVLEDRVELPRLYVGWHSPAMFAPGDAEMDLLGDLLA
                     NGKTSRLYKTLVYELRIALDVSAYQNSREIGSFFLLTATAAPGRSLTEILVRIDEELG
                     RVAVDGPSADEMERGVAQAEAHFMYRLQTVGGFGSKSDQLNAYNVYRGDPGSFSADLD
                     RYLLATPDGVRAAAKNILSPDRRVVLSVIPRGQPSQAVEGSVPVTAS"
     gene            complement(2654..>3674)
                     /locus_tag="AC16CY_15150"
     CDS             complement(2654..>3674)
                     /locus_tag="AC16CY_15150"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR3355185.1"
                     /translation="SLGFVKDLAVAGSRVSFTIELTTPACPVKDQMRDQAAAAVRALP
                     GVADVEVKLAAKVRSASAPETGRPPLPGVKNVIAVGAGKGGVGKTTVAVNLALSLARC
                     GSRVGVLDGDIYGPNVPMMFGIHTQLTTDGKKIIPAEKWGVQVVSIGFMTGDDAPIIW
                     RGPMLHGAIQQFFREVAWTDLDYLIVDMPPGTGDVALSLSQTVPVVGSIVVTTPQKVS
                     LSDSRRAIRMYQKLTIPTIGVVENMSYYACPNCHHEADIFGHGGGEALAAEMGVPFLG
                     RLPVYQPIREGGDTGVPLVVSEPGSAAARSFLTVAERTAAQVSIAAHKAAEANKGKIP
                     LIPVR"
BASE COUNT          617 a         1244 c         1228 g          585 t
ORIGIN      
        1 gtgtccgcaa tcgaggcgtg atcgccgacg accagcgtgg tgagccggga ggaatcgagg
       61 tggcgcgcgg ccgcgtcaac cacgtcggcg ccggtgacgg cgttcacttt cggcacgaat
      121 tccgagaagt acgtatcggg cagtccgtac agcgcgatct gcgcgaccga tcgggcaacc
      181 tgttgcgccg cttcgaatcc ccgcggatac ccgcgcgtca gcgacgcctt ggcgagcgcc
      241 aactcggtct cgctcggcgc gcgcaggccg cgaatggcgt cgatctcggc gagcgacagc
      301 gtaatcgcat ccgccgtggc ggaggtgtgc acgctggtct gcagcacgaa gggagaggcg
      361 ccccgccgcc agtcgaagcc ggttctggcg ccgtaggtgt accccttctc ttcccggagc
      421 ttgaggttga cgcgactcac gaactggccg ccgagcaccg cgttcatgac gagcagcgcc
      481 ggatagtccg gcgtcgcccg cccggttgtc aggtgaccga tgcgcagctc cgactgggcc
      541 gctccttcgc gagcaaccac agccagcctc gcaagcggcg ctcgtggtgg ctcggcggcg
      601 gcggcggctg caacagcgcc gccgcgcgac ggggcccagc ccccgaaacc gtcctccgca
      661 cagcgcagca gaccctcgtg gctcatggcg ccgcacacca ccagcgtcgt ccgcgggggc
      721 tggaacgtgt cgcggtgaaa cgccgcgacc tcgtccagag agatccgcct caggctcgcg
      781 tcgcctccga tgggaaggtg cccgtacggg tgcgtgccgt agaccagttt gagaaaccgg
      841 tactccgcca gcgcggaggc gagatccttc aactgtcgca ggcgatccag gcgaagttga
      901 cggactctgg tgaagtcact gtctctaagg ctcggccgcg cgaccatgtc ggccagcagc
      961 gatgcgccgc gcgatgcgaa acgcgtcaac gtcgtcaacg tgaacagcgt ggaatccccg
     1021 ccgacgtcca cgtcgtactc gcccccgatg cgggcaagag cgtcggacat ctcgatggcc
     1081 gaccgtgagc ccgagccctc gtcggccatg tcggcgacga gcgcggcgag accttccgag
     1141 tcaaccggat ccgctccgga gcctccgtca acctgaagag cgaaggtgat gaccggaacg
     1201 ctgtggtgct cgacagtcct cacgttcatg ccgtttgcga gcgtgtgccg gacgatcgcc
     1261 gggaacgtga acgccgggtc ggcgcccacc tcgggcaggc gtgacctgtc gacgcggctc
     1321 acgaggcagt gaccgggacc gagccctcga cggcctgaga gggctggccg cgtggaatca
     1381 cgctgagcac gacgcggcga tcgggtgaaa ggatgttctt tgccgccgcc cgcacgccgt
     1441 ccggcgtggc gaggagatag cggtcgaggt ccgccgagaa ggacccgggg tcgcctcgat
     1501 agacgttgta ggcgttgagc tgatcggact tgctcccgaa accgccgacg gtctgcagcc
     1561 ggtacatgaa gtgggcctcc gcctgcgcga cgccacgctc catctcgtcg gccgacggcc
     1621 cgtcgacagc cactcgcccc agctcctcgt cgattcgcac caggatctcg gtcagcgaac
     1681 gccctggagc ggcagtcgcg gtcagcagga agaagctgcc gatctccctc gagttctggt
     1741 acgcggagac gtcgagcgcg atgcgaagct cgtacaccag cgtcttgtag agtcgcgagg
     1801 tcttcccgtt ggcgaggaga tctcccagca ggtccatctc ggcatccccg ggcgcgaaca
     1861 tcgcgggcga gtgccagccg acgtacagcc gcggcagctc gacgcggtct tcgagaacca
     1921 gccggctctc ggagctgaga tcggccgacg gcgagacggg cgccggggcc gcgcccggcg
     1981 ggaggtcgcc gaagtacgcc tcggccagct cgaacccgcg cgcggcatcg atgtcgcccg
     2041 cgagcacgag ggaggcgttc gacgggtgat agtaggtgcg aaagaactcc tgcacctcgt
     2101 ccagctgcat cgcgcggatg tcatcggcgg aaccgatcgt catccagctg taggggtgat
     2161 ccggcgggta caaggccgcc gacatcgcca tcatcaccat cccgtacgga cggttttcgt
     2221 agctctgtcg ccgttcgttc agcacgacgt cgcgctggtt ctcgaagcgc tcgcgggtca
     2281 gcgccggcag gaggtatccc atgcggtccg attccatcca gagcgcgcgg tcgagcgcgc
     2341 tggtcggtac gacctcccaa taattggtgc gatcggtatt ggtcgagccg ttcaagagcg
     2401 cacccgcttg ctgcagcggc gggaagtacc cggagttgtg atgctcggag ccctcgaaca
     2461 tcaggtgctc gaacagatgg gcaaagcccg tccggccggg ccgttcgttc ttcgacccca
     2521 cgtggtacca cacgttgacc gccacgatcg gcacgtgatg gtcctcgtgc acgatgacgt
     2581 cgaggccatt gccgagcgtg cgcatgctga agggcaggtc gatggcggcg gaggtcatga
     2641 atcgtgcggc aggctaccgg accggaatca gcggaatctt gcctttgttc gcttctgcag
     2701 ccttgtgcgc cgcaatcgac acctgggcag ccgtgcgctc cgcaacggtc agaaacgacc
     2761 gcgccgccgc cgaccccggc tctgacacga cgagcgggac gcccgtatcg ccgccttctc
     2821 ggatgggttg atagacgggc aggcggccca ggaacggcac gcccatctcg gccgcgagcg
     2881 cttcgcctcc gccgtggccg aagatgtccg cctcgtggtg acaattcggg caggcgtagt
     2941 agctcatgtt ctcgacgact ccaatggtcg ggatcgtcag cttctggtac atcctgatcg
     3001 cgcggcggct gtcggagagc gacaccttct gcggcgtcgt cacgacgatc gacccgacga
     3061 caggcacggt ctggctcagg ctcagcgcca cgtcaccggt tcccggaggc atatcgacga
     3121 tcaggtagtc gagatcggtc cacgcgactt cgcggaagaa ctgctgaatc gcgccatgga
     3181 gcatcggccc gcgccagatg atcggcgcgt cgtcgccggt catgaagccg atcgacacga
     3241 cctggacgcc ccacttctcc gccgggatga tcttcttgcc gtcggtcgtg agctgcgtat
     3301 ggatgccgaa catcatcggc acgttcggcc cgtagatgtc gccgtcgagg acgcccacac
     3361 ggcttccgca cctggccagc gacaacgcca ggttgacggc caccgtggtt tttccgacgc
     3421 cgcccttgcc cgctccgacc gcaatcacgt tcttgacgcc gggcaggggc ggacgtccgg
     3481 tttctggcgc ggacgccgac cgcaccttcg cggccagctt cacctcgaca tcggcgaccc
     3541 ccggcaacgc gcgcacggcc gccgcggcct ggtcgcgcat ctgatccttc acgggacacg
     3601 cgggcgtcgt cagctcgatc gtgaaagaga cgcgcgaccc cgcgacggca aggtccttca
     3661 cgaatcccag cgag
//
LOCUS       DBNGSH010000656         2516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1766380, whole genome shotgun sequence.
ACCESSION   DBNGSH010000656 DBNGSH010000000
VERSION     DBNGSH010000656.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1766380"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2477
                     /locus_tag="AC16CY_15155"
     CDS             <1..2477
                     /locus_tag="AC16CY_15155"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355186.1"
                     /translation="IYGVEEGPSLVMELVDGPTLADRILQGPVPLDEALSLARQIAEA
                     LQAAHDKGIVHRDLKPSNIALTSEGNVKVLDFGLAKLTEASGPRPRASGPDLTASPTL
                     TSPAMMTGAGMILGTAAYMSPEQAKGRAADKRSDIWAFGCVLYEMLTGRRPFGGDDVS
                     DTLASVLARDPDWRSLPSIARPDVRMLLERCLAKDARQRFADMSVVLFLLEGSRGTHS
                     TGPETTAPISRPRERYAWSAALTLVAAAAAVLAFIHFTETPPAEPGSIRFSVSPPAGM
                     TLATGAVTSGGVVSPDGRRVVFPAGPRSGGATRLAVREFDADEAHELKGTDGAIGAFW
                     SPDSRSVGFLAGGKLQRIAVSGGPPQVVCDASGFLGGTWNGDGVIVFAPGAGALMRVS
                     ASGGTPTPVTVLDESSKETSHRQPWFLPDGQRFLYAAASASAAHSIFVGSLDGRLKTP
                     VTMSDTQASYASGYLLFVRQGTLLAQPFDVTHLTVTGEPVVAARDVATAPAAAIGDFS
                     ASTTGVLTFRRVTTGAPTQLVWVDRMGRQIETVGEPSDQTSLQLSPDGRAVAISVYDA
                     SKGSRDIWIHDLARRVRTRFTFSAGDDWTSAWSPDGRALAYSSNQTGLLDLFRKATDG
                     AGTEELLGKNIGNNRYVYGWSNDGRLLMYVTGRTRAQTGNDIWVVRRSGDDEPQPFVR
                     TSFNETDPTFSPDDRWVAYTSDESSRNEITVVPFPGPGGKWQISTGGGEQAHWSPDGR
                     ELFFLEGTKLMAVAVDGRGAALSVGPVRMLFEARFRTDNYLGYGVGSVYDVSPDGRRF
                     LINVIDSEQPAQTPITVVTNWTSLLR"
BASE COUNT          403 a          852 c          843 g          418 t
ORIGIN      
        1 aaatctacgg agtggaggaa gggccatcgc tcgtgatgga gctggtcgac ggccccacgc
       61 ttgccgaccg catcctgcag ggacccgtgc cgctcgacga ggcgctgtcg ctcgcgcggc
      121 agatcgccga ggcgctccag gccgctcacg acaagggaat cgttcatcgc gatctgaagc
      181 cgtcgaacat cgctctcacc tccgagggca acgtcaaggt tctcgacttc gggctggcca
      241 aactgacgga ggcctcaggg ccgaggcccc gggcctcagg gccggacctc acggcgtcgc
      301 ctacgctcac ctcgccggcc atgatgaccg gcgccggcat gattctcgga acggcggcct
      361 acatgtcgcc cgagcaagcc aaggggcgcg cggccgacaa gcgcagcgac atctgggcgt
      421 tcggctgcgt gctctacgag atgctgaccg ggcgacgtcc cttcggcggc gacgacgtct
      481 cggacacgct ggcgtcggtg ctggcgcgtg atccggattg gcgctcgctg ccctctatcg
      541 cgcggcctga cgtgcgcatg ctgctcgaac gatgcctcgc caaggatgca cggcagcgct
      601 tcgcggatat gtcggtcgtg ctgttcctcc tggaggggtc gcggggaaca cactcgacgg
      661 gtccggaaac gacagcgccg atctctcgcc cacgcgagcg gtatgcgtgg agtgcggcgc
      721 tcacccttgt ggcggcggcg gccgccgtgc tcgccttcat tcacttcacc gagacgccac
      781 ccgccgagcc cgggagcatt cgtttctcag tctcaccacc tgccggcatg acgctggcta
      841 ccggcgccgt caccagcggc ggcgtggtct cgccggacgg ccggcgcgtg gtctttccgg
      901 ctggtccgcg cagcggcggc gccacacggc tggccgttcg ggaattcgac gcagacgagg
      961 cgcacgaact gaaaggaacc gacggggcga tcggcgcgtt ctggtcgccc gacagccgct
     1021 ctgttggttt tctcgccggc ggcaagctgc agcggatcgc cgtgtcgggt ggcccgccgc
     1081 aggtggtctg cgacgcgtcg ggatttctcg gcggcacgtg gaacggcgac ggcgtcatcg
     1141 tgtttgcgcc cggcgccggt gcactgatgc gtgtgtccgc gagcggggga acgcccacac
     1201 ctgtaacagt gttggacgag tcgagcaagg aaacgtcgca cagacaaccg tggttcctgc
     1261 cggacggcca gcgcttcctg tacgccgcgg cctcggcgtc cgccgcacac tccatcttcg
     1321 tcggatcgct cgatggccga ctcaagacac ccgtcaccat gtcggacacg caggcgagct
     1381 acgcaagcgg ctatctgctc ttcgtccggc agggcaccct tctggcgcaa ccgttcgatg
     1441 tcacacacct gacggtgacg ggcgagcccg tggtggcggc gcgtgatgtg gcgaccgccc
     1501 ccgccgccgc aatcggcgac ttctccgcat ccacgactgg tgtcctgaca ttccggcgcg
     1561 tgacgacggg cgcacccacg cagctcgtgt gggtcgatcg catggggcgg caaatcgaga
     1621 ccgtcggtga gccgtccgat cagacctcgc tgcagctctc ccccgacgga cgagccgtcg
     1681 cgatcagcgt ctacgacgcg tcgaaggggt cgcgtgatat ctggatccat gacttggcca
     1741 ggcgcgtgcg gacgcgcttc acgttcagcg ccggagacga ctggacctcc gcgtggtcac
     1801 ctgacggccg ggccctcgcg tacagctcga accagactgg attgctcgac ttgttcagga
     1861 aggcaaccga cggcgccggc accgaggagt tgctgggcaa gaacatcggc aacaacaggt
     1921 acgtctacgg ctggtcgaat gacggccggt tgctgatgta cgtcacaggc agaacgcggg
     1981 cccagaccgg gaacgacatc tgggtcgtgc ggcgatcggg cgacgacgag ccgcagccgt
     2041 tcgtgcgaac gtcgttcaac gagaccgacc ccacgttctc acctgacgac cgctgggtgg
     2101 cctacacctc ggacgagtcc agccgcaacg aaatcaccgt cgtccccttc cccggacctg
     2161 gcggcaaatg gcagatctcg acggggggag gcgagcaggc gcactggagc cccgacgggc
     2221 gagagctgtt ctttctcgag ggtacgaagt tgatggccgt cgccgtggat ggccgcggcg
     2281 ctgcgctgag cgtcggcccc gtacgaatgc tgttcgaggc gcgattccgc acggacaact
     2341 atcttggcta tggcgtcggc tcggtgtacg acgtctcgcc cgacggccgc cgcttcctga
     2401 tcaacgtcat cgacagcgag cagcccgcgc agacgcccat caccgtcgtc accaactgga
     2461 cgtcgttgct gcgttgatcg tcgctacttc ttcgctcgcg cgagctcctc gtcgat
//
LOCUS       DBNGSH010000657         1586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4144159, whole genome shotgun sequence.
ACCESSION   DBNGSH010000657 DBNGSH010000000
VERSION     DBNGSH010000657.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4144159"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="AC16CY_15160"
     CDS             <1..199
                     /locus_tag="AC16CY_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HIR3355187.1"
                     /translation="LHRPDVLPVGDLGIVKAVKQAYRLRSRPTPARLTKIGEVWRPYR
                     SVACWYLWASLDAAPLEDEGA"
     gene            223..>1586
                     /locus_tag="AC16CY_15165"
     CDS             223..>1586
                     /locus_tag="AC16CY_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3355188.1"
                     /translation="MRNKTYSTLFVVLLAVLIGLGALLTRGGSSSSADAPSIPFEKYT
                     LPNGLDVILSRDERIPMVSVNLWYHVGPANEEPGRTGFAHLFEHMMFQSSKHVPPDAH
                     FKLLEAAGASDVNGTTDFDRTNYFQTVPANQLELALWLESDRMGFLLEKVDQAMLSNQ
                     QDVVRNERRQSYENEPYGLAEEALVQALYPRGHPYYGNVIGSHEDIQAAKLDDVRTFF
                     RQYYSPNNASLAIVGDFDPAQARSLVEKYFSTLKRGPEVPAITAETPKITSERRKVVR
                     DRIELPRVYMAWLTSPIFKPGDAEAALAATILGGRRSSRLYKKLVYEKQIAQTVSAQQ
                     YSLILGSIFQIEATARPGHTVEELEKALEEELAAFRASPPTPEELDRARNTIETNIVG
                     GLEFLGGFGGKADRLNSYNHYLGTPDYLRKDLERYRTADVASVHAFAREQLTADARVV
                     IHVVP"
BASE COUNT          285 a          536 c          504 g          261 t
ORIGIN      
        1 gctgcaccgc cccgacgttc tgccggtcgg cgacctcggc atcgtgaaag cggtcaagca
       61 ggcgtataga cttcgttcgc ggccgacgcc cgcgcggctc acgaagattg gagaggtgtg
      121 gcggccgtac cggtcggtgg cctgctggta cctctgggcg agccttgacg cggcgccact
      181 cgaggacgag ggcgcctgat gccgcgctga gcacacgcac tcatgcgaaa caagacctac
      241 tcgacccttt tcgtcgtcct gcttgccgtc ctgattggcc tgggggcgct gctcactcgc
      301 ggtggatcgt cctcgtccgc cgacgccccg agcattccgt tcgagaagta cacgctgccg
      361 aacggactcg acgtgatcct gagccgcgac gagcgcatcc cgatggtatc ggtcaacctc
      421 tggtatcacg tggggcccgc caacgaggag ccgggacgca ccgggttcgc gcacctgttc
      481 gagcacatga tgttccagag ctcgaagcac gtgccgcccg acgcgcactt caagctgctc
      541 gaggccgccg gcgcgagcga cgtcaacggc acgaccgatt tcgatcgcac caactacttt
      601 cagaccgttc ccgccaacca actggagctg gccctgtggc tcgagtcgga tcgcatgggc
      661 ttcctgctcg agaaggtcga tcaggcgatg ctctcgaacc agcaggacgt cgtccgcaac
      721 gagcgacgcc agagctacga aaacgagcca tacggcctcg cggaggaagc gctcgtgcag
      781 gcgctctatc cgcgcggtca tccctactac ggcaacgtca tcggctcgca cgaggacatt
      841 caggcggcga agctggacga cgtccgtacg ttcttccgcc agtactactc gcccaacaac
      901 gccagcctcg ccatcgtcgg cgatttcgat ccggcgcagg cccgctcgct cgtcgagaag
      961 tacttcagta cgttgaagcg aggtcccgag gttcccgcga tcacggccga gaccccgaag
     1021 atcacttcgg agcggcggaa ggtcgtccgc gatcgcatcg agctgccgcg cgtgtacatg
     1081 gcgtggctca cctcacccat cttcaagccc ggagacgcgg aagcggcgct ggccgccacc
     1141 atcctgggcg ggcggcgctc gagcaggctg tacaagaagc tggtctacga aaagcagatt
     1201 gcgcagacgg tctcggcgca gcagtattcg ctgatcctcg gatcgatttt ccagatcgag
     1261 gcgaccgcgc gcccggggca cacggtcgag gagctcgaga aggcgttgga ggaggagctc
     1321 gccgcatttc gtgcgtcgcc cccgactccc gaggagctcg accgggctcg caacacgatc
     1381 gagaccaaca tcgtcggcgg cctcgagttt ctcggcggct tcggtggaaa ggccgaccgg
     1441 ctgaactcgt acaaccacta cctcggcacg cccgactacc tgcgcaagga tctcgagcgg
     1501 taccggacgg cggacgtggc gtccgttcac gccttcgcgc gcgagcagtt gacggcggac
     1561 gcgcgcgtcg tgatccacgt cgtgcc
//
LOCUS       DBNGSH010000658         2918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2615474, whole genome shotgun sequence.
ACCESSION   DBNGSH010000658 DBNGSH010000000
VERSION     DBNGSH010000658.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2615474"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="AC16CY_15170"
     CDS             complement(<1..1907)
                     /locus_tag="AC16CY_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3355189.1"
                     /translation="MPSRLLRLLSLGVVALVCVLALTTSQAQQPAADVAIDNDDIGGV
                     VTGPKGPEAGVWVIAETRDLPVRYIKSVVTDDRGRFVVPDLPPANYSVWARGYGLVDS
                     AKATAKPGQRLAIAATPAPNEAAAAHYYPAIYWYSMLKIPAADQFGGKSSIPARLTQQ
                     NWLGSVKNLGCIGCHQLGQQSTRTIPASLGTFASGADAWRRRVQSGQSGQQMLGQLNG
                     LGDASFASYGDWTDRIAKGELPFAKPPRPQGVERNIVVTLRDWMNDKQYLHDLIASDR
                     RYPTVNAYGPLYGSPEYSSDMLPILDPVKNVATTFKAPVRDPQMPLNLGPGHAAALDA
                     LEPSPYWGRERIWETRVNNHNSMFGRDGRLWLAASVRGAENPAFCKAGSDHPSAKAFP
                     MDRAVRHISVFDPKTQKYTFVDTCFSTHHLQFGYDANDTIWTSGGGPVVGWLNTKMFD
                     QTGDAARSQGWTALVLDTNGNGKRDAYVEADQPPDPAKDTRITSAFYAVMPSPADGSI
                     WGSTLGVPGAVVRIAPGSNPPETALAEIYRVPMPAFGARGADIDRQGVVWVSLASGHM
                     GSFDRRKCKGPLNGPKATGDHCPEGWSFHQYPGPGFRGIGENSAESSYYSWVDQHNTF
                     GLGNDVPMSTG"
     gene            2115..2203
                     /locus_tag="AC16CY_15175"
     tRNA            2115..2203
                     /locus_tag="AC16CY_15175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2149..2151,aa:Leu,seq:taa)
     gene            complement(2412..2810)
                     /locus_tag="AC16CY_15180"
     CDS             complement(2412..2810)
                     /locus_tag="AC16CY_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355190.1"
                     /translation="MVKVPIVAMLIAASLAVPAPAQSQVSVQVSIGQPPPVIVAQPVL
                     VPVQASPVYYAPSYGADLFFHDGRYYTVRDDQWFYAARLNAPWVAITVGRVPQQILAI
                     PVTYYRVPPGHLKHKGAAHCPPGQAKKGRC"
BASE COUNT          465 a          931 c         1002 g          520 t
ORIGIN      
        1 ttgccggtgg acatcggcac gtcgttgccg aggccgaagg tgttgtgctg atcgacccac
       61 gagtagtaac tcgactcggc gctgttctcg ccgatgccgc ggaagcccgg tcccggatac
      121 tggtggaacg accatccctc ggggcagtgg tcgcccgttg ccttggggcc gttgagcggt
      181 cccttgcact tccggcggtc gaagctgccc atgtgcccgc tggcgagcga gacccagacg
      241 acgccctgcc gatcgatatc agcgccgcgc gcgccgaacg ccggcatcgg cacgcggtag
      301 atctcggcga gcgccgtctc tggtggattc gagccgggcg caatccgcac gaccgcgcct
      361 ggcacgccca gcgtcgaccc ccagattgag ccgtcggccg ggctcggcat cacggcgtag
      421 aaggcggacg tgatgcgcgt gtccttggcc gggtcgggcg gctgatcggc ctcgacgtag
      481 gcgtcgcgct tcccgttgcc gttggtgtcg agcacgagcg ccgtccaccc ctgcgaccgc
      541 gcggcgtcgc ctgtctggtc gaacatcttc gtgttgagcc agccgacgac gggacctccg
      601 ccgctcgtcc agatcgtgtc gttggcgtcg tagccgaact gcagatgatg ggtcgagaaa
      661 caggtgtcca cgaacgtgta cttctgcgtc ttcggatcga agaccgagat gtgccggacg
      721 gcgcgatcca tcgggaacgc cttcgccgaa gggtggtccg accctgcctt gcagaacgcc
      781 gggttctccg cgcctcgaac cgaggctgcg agccaaagac gcccgtcgcg gccgaacatc
      841 gagttgtggt tgttgacgcg cgtttcccag atgcgctcgc ggccccaata gggcgacggc
      901 tccaaggcat cgagcgccgc cgcgtggccg ggaccgaggt tcagcggcat ctgcggatcg
      961 cgcacgggcg ccttgaaggt ggtggcgacg ttcttcaccg gatcgaggat gggcaacatg
     1021 tccgaactgt attctggcga cccgtagagc ggcccgtacg cgttgaccgt ggggtagcgc
     1081 cgatcgctcg cgatcagatc gtgcaggtac tgcttgtcgt tcatccagtc gcggagcgtg
     1141 acgacgatat tccgctcgac gccctggggc cggggtggct tcgcaaacgg cagctcgcct
     1201 ttggcaatgc gatccgtcca gtcgccatag cttgcgaacg acgcgtcgcc gaggccgttc
     1261 aactggccga gcatctgctg gcccgactgg cccgactgca cccgccgccg ccatgcgtcg
     1321 gccccggagg cgaaggtgcc gagcgaggcg ggaatcgtgc gcgtggactg ctggccgagc
     1381 tgatggcagc cgatgcagcc gaggttcttc accgagccga gccagttctg ctgcgtcagc
     1441 cgtgcgggga tgctgctctt gccgccgaac tgatcggcgg ccggaatctt cagcatcgag
     1501 taccagtaga tcgcggggta gtaatgcgcg gccgccgcct cgttcggcgc gggcgttgcc
     1561 gcgatcgcca ggcgctgtcc tggtttcgca gtggccttgg cgctgtcaac gagaccgtag
     1621 cctcgcgccc agaccgaata gttcgccggc ggcaggtccg ggacgacgaa gcggccgcgg
     1681 tcgtccgtga cgacgctctt gatgtagcgc accgggaggt cgcgcgtttc ggcgatcacc
     1741 cagacgccgg cctcgggacc cttcggtccc gtcaccacgc cgccgatgtc gtcgttgtcg
     1801 atcgcgacgt cagccgcggg ttgctgggcc tgtgacgtcg tcaaggccag cacgcacacc
     1861 agtgcgacga cgccgagact gagaaggcgg aggagtctgc tgggcatgac caatgctcct
     1921 gtgatgttgt ggaggaagtc tacgcggccc aacggtctac gggcaacaga ggtacgtagt
     1981 gtccggcttc agccggacct tgggcgttga cgtacgtagt gtccggcttc agccggacgg
     2041 acagtcgtag tgtccggctt cagccggact cgggcggacg tgagatcgat gtaagctgcg
     2101 accaacgcat tgcggccggg gtggcgaaac tggcagacgc acgggactta aaatcccggg
     2161 tcctgaaaag gacgtgggag ttcgatcctc ccccccggca ccaatctcgt cgccgtgcct
     2221 atttgatccg cggtcccttg aaatcgccct cccgttgggg aagagactag aggcacgccg
     2281 actctatccg ggaagcgaaa cgctggcggg ttttcgggga cgcgggctct tgcgacttcg
     2341 ccatcctgtc gcactgctga aacccggcgg cgcttcccgt ggggccgcgc cgccggatgg
     2401 ctggtgtgac gtcagcagcg gcctttcttc gcctggcctg gcgggcagtg cgcagcgccc
     2461 ttgtgcttca gatgtccggg cggcacgcga tagtaagtaa cgggaatcgc gaggatctgc
     2521 tgcggcaccc tgccgactgt gatggcgacc cacggcgcgt tgaggcgcgc cgcatagaac
     2581 cactggtcat cgcggacggt gtaatagcgc ccgtcatgaa agaacagatc agcgccgtac
     2641 gacggcgcgt aatagacggg cgaggcctgc accggcacca gtacgggttg cgcgacgatg
     2701 accggcggtg gctgcccgat actgacctgc acgctgacct ggctttgcgc aggcgcggga
     2761 accgcgaggc tcgcggcgat gagcattgct acgatcggca ccttgaccac ggtctcctcc
     2821 acatcgcgtt ctgggggtga cgatgtggga gcaactcgag tgccagctct tctcttccgc
     2881 agatttcgtc gaattgcacg accggctgag gcttcacg
//
LOCUS       DBNGSH010000659         8027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1766624, whole genome shotgun sequence.
ACCESSION   DBNGSH010000659 DBNGSH010000000
VERSION     DBNGSH010000659.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1766624"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="AC16CY_15185"
     CDS             <1..485
                     /locus_tag="AC16CY_15185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3355191.1"
                     /translation="GPLLRDVHLVIANEEDLQSMLGIHVAGTDATAGHLDVDGFREAA
                     ERVTTGFGPQTVAITLRESVSASHNGWSALLWDAQANRMLTSQRYDIAVVDRIGSGDS
                     FAAGLIYGLVTGRTPEHALRFAVAASALKHTIPGDFNRASVEEVDRLAAGDASGRVQR
                     "
     gene            complement(585..1304)
                     /locus_tag="AC16CY_15190"
     CDS             complement(585..1304)
                     /locus_tag="AC16CY_15190"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3355192.1"
                     /translation="MERTVGCLFGVVLLGACDGAVGRESPTGPTPGISLPTPGGLTGV
                     YGLSGVVTAVGVPVAGATVALLTFEAGALIESTLTDGNGSYTLAAVRNVSPYSGALVS
                     VSKPGYFTNTRYVPMTRDETSDFDLVRASHISVGQQIRSRPGDARCASLGYGGGGGAV
                     CQQFAVTVPSSGTLEVTVSSSPASAFDTTVLEPHGTIGAYVAAPTTPLRLALQVVAGL
                     TYQIDVVPIGVPEFELTTALR"
     gene            complement(1365..2327)
                     /locus_tag="AC16CY_15195"
     CDS             complement(1365..2327)
                     /locus_tag="AC16CY_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3355193.1"
                     /translation="MDCKAGRRGPFAGGLLVVVVSVASHGCGDSAGLPTSGPSPSDPA
                     SPPVRTLRINGPAVLSVIGETSQLRATATFSDNSTRDVTSEATWRSLNPSVVNVSPTG
                     LVTVAGYGASNVEATYRAGRASLPVFVTPPGTFFVRGRVREPGRGGLAGVRVFDPQSG
                     RSTFTSGEGEFALGGLTNARLMFDIEAYESREADLPPNVESDVALQRVIRLTVGESVT
                     PAQLAPHDMSYGVGGDQCQPCRLIRVVSPSAGTLRLRLAWSDPRVPLSVWANGQLFKG
                     ASSTELTADVPVGAGELVVYVGAIPAVTLADRVYLPFTLTASMP"
     gene            2368..4563
                     /locus_tag="AC16CY_15200"
     CDS             2368..4563
                     /locus_tag="AC16CY_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3355194.1"
                     /translation="MEIPPKKLPIFSFGPFLLDLTEHALLRDGRPVPLTPKVFEVLRL
                     LVQNAGHLVLKEQLLKEVWPDTFVEEANLNRAVSVLRKALGESQSDRYIETVPKRGYR
                     FVAHVTEGFGVSSLVESTPAKGAPRVRALAGPPRVRALAGPLRMAGVASALLASVAVA
                     YVARGSHEAAPPLSRGPVHRQVTFTGTEGGAPALSPDGRSIAYVSFGSRVHTLMVKDT
                     TGGQPVPVDSAPEIARPRPSPDGSRLLYWKRGAGIPGMYVVPRTGGTSRLVAPGLFVA
                     CWSPDGSTIAAAQFLQGKILFVSTSGDGPRSVALESTSSWIYDIDWSPATNHLLFSSS
                     DGRGGFSIWTMQPDGSEQTKVLADRVEIAAVRWAPDARSIYYFRRVNQTMSLHKLARQ
                     SRDATWEALPGPLLTGLETDGSFGLSVDGRSLVYSRAPFLTNLWTVQTGDEGTGDSVV
                     TRQLTSGTSFIERPRISPDGKRVAFNVGYESHANLHVMSIEGGPSTQLTFLDGMNVGP
                     AWSPDGEQIAFASTEGITPRVWVVGARGGAPRAVSSSDLSGEGLEVSWGPGSQLLYQQ
                     TGNRNFSVLDLGTGHERPLIKDSSVGWAFSPVHSPDGRRIAVAWNRRPARGIWIIDTR
                     DSSERLLYAAPPPLLLAWSADGTSIYALEGGSPVYRGLIAHRGESLTDARIVKVSALD
                     GHVQSTVTLPFEEVGGVSVSPDGRTFVCTVYSSRSDIWVVDDFDITKQP"
     gene            4628..6700
                     /locus_tag="AC16CY_15205"
     CDS             4628..6700
                     /locus_tag="AC16CY_15205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3355195.1"
                     /translation="MRSRELNCALAAFAIIAVVAGCSRTTTQADKTAARAVTFNGDIA
                     PILFDNCASCHRPIDDAAPLPGRGSATREGGPAATSEPASPKPSAKAGSIADPICVAG
                     APFSVLDYDSVRRNARAIASAVQRRAMPPWLPEPGYGVFAGERRLRDDQIALIAKWVE
                     SGAPEGNPADAPTPPSFAGGWQLGTPDLVLTLPEAYELQPFDSAQGRPQPGTRDVFRN
                     FVIPVPITTTRYVRAVEFRADRPQALHHADVAIDLGRVSRALDRAEPGPGFAVMDGAQ
                     VYNVYGWSPGKVPVMEPSDNAWTLEPGADLILLLHMIAGAQSETVQPAIGLFFSDTPP
                     TRTPISVKLEAPGIAIPAGEADYVVEDSYVLPVDVEAVSIYPHAHYLGKEMRGTATLP
                     DGSQKDLLSIRQWDFRWQDRYRYRSPVFLPQGTRLSMRFTYDNSAANPNNRFDPPQRV
                     RSGPRSTDEMGQLWIEVVPRRAGDAALLNADFVRRSLAAAIASAELDVRLDPHTAAAR
                     NVLATSYLSARRVDEARAQLEEALRLDPRHAEARSNLGVVLQAQGYLAEGMKHLRTAV
                     QLKPKDDSVRFNLGGGLLALGQPDAAIREFRAAIALNPDNADAHFNLGVILGPRNQLD
                     DAIAHLQRAVEINPLNADAYHNLAVAYGLQGRIDAAIAAAQTAVRLKPASAAAQEQLQ
                     RLLAARKR"
     gene            complement(6762..>8027)
                     /locus_tag="AC16CY_15210"
     CDS             complement(6762..>8027)
                     /locus_tag="AC16CY_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3355196.1"
                     /translation="TVFGGSLSETNAAKIVKIMDLAVKMGAPVIGLNDSGGARIQEGV
                     VSLGGYADIFLRNTLASGVVPQISAIMGPCAGGAVYSPAITDFTVMVKGTSYMFVTGP
                     DVIRTVTHEEVTKEELGGAMTHNEKSGVAHFAAADDEAAILLIRELLSFLPGNNVDDP
                     PRREISDPADREDEALDRIVPAAANQPYDMLDVIHAVVDDRELLEVHEHFAKNIVVGF
                     ARLGGRPVGVVANQPAHLAGTLDIDASVKAARFVRFCDAFNIPLVTFEDVPGFLPGTV
                     QEWGGIIRHGAKLLFAFAEATVPKLTVITRKAYGGAYCVMSSKHIRTDANFAWPSAEI
                     AVMGAEGAVNILYKRELEAAGDVAAARAARVAEYREKFASPYIAAQRGFIDEVIPPRQ
                     TRRKLITALAALDTKRDRNPPKKHGNIPL"
BASE COUNT         1380 a         2653 c         2630 g         1364 t
ORIGIN      
        1 tgggccctct cctccgtgac gtgcacctcg tcatcgccaa cgaagaagat ctgcagtcga
       61 tgctcggcat ccatgtcgcg ggaaccgatg cgacggccgg gcatctggat gtcgacggct
      121 tccgcgaggc ggccgagcgc gtcacaaccg gcttcggccc gcaaaccgtt gcgatcacgc
      181 tgcgcgagag cgtctcggcg agccataacg gctggagcgc gctcctctgg gacgcgcagg
      241 cgaaccggat gctgacgagc cagcgctacg acatcgccgt cgtcgatcgg attggcagcg
      301 gcgacagttt cgcggccggc ctgatctacg gcctcgtgac tggccggacg cctgaacacg
      361 cgctgcggtt cgcggtggcg gccagcgcgt tgaagcacac gatccccgga gacttcaatc
      421 gtgcgagcgt cgaggaggtc gatcgtctcg cggcgggaga cgcctcgggg cgcgtgcaac
      481 gctaaagcgg gacacgaatg cccttggagt ttccaatctg acgattcggg ttctaccgat
      541 gtgacgacag tggaatcacg ccgttgacca gcccacgaag ggaatcatcg caacgccgtg
      601 gtcaattcaa actccggaac gccaattgga acgacgtcaa tctggtacgt caggcccgcc
      661 acaacttgga gcgcgagtcg tagcggtgtc gtaggtgcag caacgtaggc accgattgtt
      721 ccgtgtggct cgaggacggt tgtgtcaaag gcggacgcgg gactcgagga tacggtgacc
      781 tcgagcgttc ccgatgacgg cactgtcacg gcgaactgct ggcaaacagc accgcccccc
      841 ccgccatatc ccaagctggc gcagcgcgcg tcgcctggtc gacttcggat ttgctgcccc
      901 accgatatgt gcgaggcacg taccaaatcg aagtccgatg tctcgtcccg agtcatgggc
      961 acatacctgg tgtttgtaaa gtaccccggc ttggacacac tcacgagcgc gccagaatac
     1021 ggcgagacgt tcctaactgc tgccaaagtg taggagccgt ttccatcggt cagcgtcgac
     1081 tcaatcaacg ctcccgcttc gaaggttaac agcgcgactg tcgcgccagc gaccgggacg
     1141 ccgaccgctg taacgacgcc ggacagcccg tacactcccg tcaatccgcc cggagtgggc
     1201 agcgatatgc ctggtgtggg cccagtaggt gattctcgtc ctacagcacc gtcacaggct
     1261 ccgaggagca cgacgccgaa cagacaaccc accgtgcgct ccatgatctg cccctcatgc
     1321 gacgccaagg ttttggtgtg caacgctgat tgccatgggt tcggtcaagg catcgaggcg
     1381 gtgagcgtga acgggaggta gaccctgtcg gccagggtca ccgcgggaat cgctccgacg
     1441 tataccacga gttcaccggc gccaaccggc acgtcagccg tcaactcggt cgacgacgcg
     1501 cccttgaaca gctgtccgtt cgcccacacg ctcaacggaa cgcgcggatc gctccacgct
     1561 agtcgcaaac gaagcgtgcc cgcgctcgga ctgacgaccc tgatcagcct gcagggctgg
     1621 cattgatcgc cccccacccc gtacgacatg tcgtgtggcg cgagttgcgc cggcgtaacc
     1681 gactctccaa cggtcaagcg aatgacccgc tggagcgcca cgtccgactc cacattcggg
     1741 ggcaggtctg cctcgcggct ttcgtacgcc tcgatgtcga acatgagacg cgcgttcgtg
     1801 agcccgccca gtgcgaattc accctctccc gacgtgaacg tcgacctgcc ggactgtggg
     1861 tcgaagacgc gtacaccggc gagcccaccc ctgcccggct ctctcacccg tccacggaca
     1921 aagaacgtac ctggcggcgt cacgaagacc ggcagtgacg ctctccctgc ccggtacgtc
     1981 gcttccacgt tgctcgctcc gtaaccggcc accgtcacga ggccggtcgg cgacacattc
     2041 acgacggatg ggttgagcga gcgccaggtc gcttcggagg tgacgtcccg ggtcgagttg
     2101 tcggagaagg tggccgtcgc cctcaactgg ctggtttctc cgattaccga taagacggcc
     2161 ggaccattga ttctcagggt gcggaccggc ggcgatgcgg ggtccgatgg cgacggaccc
     2221 gacgttggaa gacccgcaga gtcgccgcag ccgtgactgg caacggacac gacgaccacc
     2281 agaagacctc cggcaaacgg gcctctccgt cctgctttgc agtccatcgg ggtattcgat
     2341 taggcttgcc gcgacggacg gacgtccttg gaaatacctc caaaaaaact cccaatcttc
     2401 tccttcgggc cgttcctcct ggacctgacc gagcacgccc tgctccgtga cggccggcca
     2461 gttccgttga caccgaaggt cttcgaggtc ctcaggttgc tcgtccagaa cgcagggcat
     2521 ctggtgctga aggaacaact cctgaaggag gtctggccgg atacgttcgt ggaggaagcc
     2581 aacctgaatc gggcagtatc ggtactgcgg aaggccctcg gtgagagcca gtctgacagg
     2641 tacatcgaga cagtcccgaa gcggggctac cgattcgtgg cgcacgtcac tgaggggttc
     2701 ggtgtctcgt ccctcgtcga gtcaacccct gccaaaggtg cgccgcgcgt aagggcactg
     2761 gccggcccgc cgcgcgtaag ggcactggcc ggcccgctgc ggatggcggg tgtcgcgagt
     2821 gcgcttctcg cctccgttgc cgtcgcctac gtcgctcgcg gctctcacga ggctgcgccg
     2881 ccgttgtcgc gcggcccagt gcaccgccag gtcacgttca cgggaacgga gggcggggcg
     2941 cctgccctgt cgcccgacgg ccgcagcatc gcctacgtgt cgttcggctc ccgcgtccac
     3001 acgttgatgg tgaaagacac gacgggcggc cagcccgtgc cagtagacag tgcgccggaa
     3061 atcgccaggc cccggccgtc gcccgatggt tccaggttgc tgtattggaa gcgcggcgct
     3121 ggaatacctg gcatgtatgt cgttccgcgc accggtggca catcgcgtct cgtcgctccc
     3181 ggtctcttcg tggcgtgctg gtctcccgac ggttccacga ttgccgctgc gcagttcctg
     3241 caaggaaaga tcctgttcgt cagcacgagc ggagacggac cgagatcggt tgctctcgaa
     3301 agcacttcca gctggatcta cgacatcgac tggtcgccgg ccacgaacca cctgctcttc
     3361 agcagcagcg acggtcgggg aggattctcg atctggacga tgcagccaga cggcagtgaa
     3421 cagacgaagg tgctcgcgga tcgggttgag attgcagccg tgcgttgggc gccggacgcg
     3481 cggagcatct actattttcg ccgtgtcaac cagacaatgt cgctccacaa gctcgcacgg
     3541 caatcgcgtg atgcaacatg ggaggcgttg ccggggccgt tgctcacagg actcgagacg
     3601 gacggatcct tcggcttgtc ggtcgacgga aggagcctcg tctactcgcg cgcgcccttc
     3661 cttacgaacc tgtggactgt ccagacgggc gacgagggca ccggcgacag cgtggtcacc
     3721 aggcaactga ccagcggcac gtccttcatc gagcggccga ggatttctcc cgatgggaag
     3781 cgcgtggcgt tcaacgtggg ttacgagtcg cacgccaacc tgcacgtcat gtccatcgag
     3841 ggaggtccat cgacacagct gacgttcctc gacggaatga atgtcggccc cgcctggtcc
     3901 ccagacgggg agcagatcgc gtttgcgtcg accgagggaa tcacgccacg ggtgtgggtc
     3961 gtcggcgccc gcggcggcgc tcccagggcg gtctcgtcga gcgacttgag cggcgagggc
     4021 ctcgaggtgt cgtgggggcc gggatcgcag ctcctgtacc agcagacggg caatcgcaat
     4081 ttcagcgtcc tcgacctcgg gacgggacac gaacgcccgc tgatcaaaga cagttcagtc
     4141 ggatgggcgt tttccccggt tcactccccc gacgggaggc gtattgccgt cgcctggaac
     4201 cgtcgtccgg cgcggggcat ttggataatc gacacacgag attcaagcga gcgactcttg
     4261 tatgcggctc caccacctct tctcctcgct tggtccgctg acgggacgtc catctacgcg
     4321 cttgaagggg gcagccccgt gtatcgaggc ctgatcgcgc accgcggcga atcattgact
     4381 gacgcacgca tcgtgaaagt ctccgcactc gacggccacg tgcagtccac ggtgacactc
     4441 ccgttcgaag aagtcggcgg agtcagcgtg tctccagacg gccggacgtt cgtgtgcacg
     4501 gtctattcgt cgcgatccga catatgggtc gtggacgact tcgacatcac gaagcaaccc
     4561 tagctctggg ggcaccagtc gccacatgca gttccaggtg gccccgaggt cacctacaat
     4621 ccagcgtatg cggagtaggg agctgaattg cgcactcgct gccttcgcga tcatcgccgt
     4681 cgtcgctgga tgctcgcgga cgacgacaca ggcggacaag actgcagcgc gcgcggtgac
     4741 gttcaacggc gacatcgccc cgatcctctt cgacaactgc gcgagctgcc atcgcccgat
     4801 cgatgacgcc gcgccgctgc ctgggcgcgg aagcgcgacg cgcgaaggcg gccccgctgc
     4861 gacgtcagag cctgcctcgc cgaagccttc ggcgaaggcg ggttccatcg ctgatccgat
     4921 ctgcgtggct ggtgcgccgt tcagcgtgct cgactacgac tcggtccgtc gcaacgcgcg
     4981 cgcgatcgcc tcagccgtgc agcgccgcgc gatgccgccg tggctgcccg agcccgggta
     5041 cggcgtgttt gccggcgaac gccggctccg cgacgatcag atcgcgctca tcgcgaagtg
     5101 ggtcgagagc ggcgcgcccg agggaaatcc ggccgacgcg ccgacgccgc cgtcgtttgc
     5161 cggcggatgg caactgggca cgcccgatct cgtactgacg ctgcccgagg cgtacgagct
     5221 gcagcccttc gactccgctc agggcaggcc ccagccagga acccgcgacg tgttccggaa
     5281 cttcgtcatt ccggtaccga tcaccacgac gcgctacgtg cgagccgtcg agttccgcgc
     5341 cgatcgccca caggcgctgc accacgccga cgtggccatc gatctcgggc gcgtgtcgcg
     5401 cgcgctcgat cgtgccgagc cgggtcccgg gttcgcggtg atggacggcg cccaggtgta
     5461 caacgtgtac ggctggtcgc cgggcaaggt gccggtgatg gagccgtccg acaacgcctg
     5521 gacgctcgag cctggcgccg acctgatcct gctgctccac atgatcgcgg gtgcgcagag
     5581 cgaaacggtg cagccggcga tcggcctgtt cttctccgac acgcctccga cgcgcacgcc
     5641 gatcagcgtg aagctcgagg cgccggggat cgccattccc gccggcgagg cggactacgt
     5701 cgtggaggac agctacgtgc tcccggttga cgtcgaggcg gtgagcatct atccgcacgc
     5761 acactatctc gggaaggaga tgcgcggaac ggcgacgctt ccggacggct cgcagaagga
     5821 tctgctgtcg attcgccagt gggactttcg ctggcaggac cggtatcgct atcgatcgcc
     5881 tgtgttcctg ccgcagggca ctcggctgtc gatgcggttc acctacgaca actcggcggc
     5941 gaatccgaac aaccgtttcg atccgccgca gcgcgtgcga tcgggaccgc gctcgaccga
     6001 cgagatgggc cagttgtgga tcgaggtcgt gccgcgccgc gcaggggacg cggcgctcct
     6061 caacgccgat ttcgtccgcc gctcactggc ggctgccatc gcgtcggccg agttggacgt
     6121 ccggctcgat ccgcacactg ccgccgcgcg gaacgtgctg gcgacgagtt acctgtcggc
     6181 gagacgcgtg gacgaggcgc gggcgcagct cgaagaggcg ctgcgcctgg atccgcgcca
     6241 cgcggaggcg cgcagcaacc tcggggtcgt gctgcaggcg caggggtatc tggccgaggg
     6301 gatgaagcac ctgcgcacgg ccgtgcagct gaagccgaag gacgactccg tgcgcttcaa
     6361 tctcgggggc ggtctgctgg cgttgggcca gcccgatgcg gcgatacgcg agttcagggc
     6421 ggccatcgcg ctcaatccgg acaacgcgga cgcgcacttc aacctggggg tgatcctcgg
     6481 gccgcgcaac caactggacg atgcgattgc gcacctgcag cgcgcggtgg agatcaaccc
     6541 gctcaacgcc gatgcgtatc acaatctggc agttgcctac ggactgcagg ggcgaatcga
     6601 cgccgcgatt gcggcagcgc agacggccgt gcgcctgaag cccgcttcag ccgcagcgca
     6661 ggagcagttg cagcggctgc tcgccgcgcg caaacggtga cagcgggtcc cagcccgtcg
     6721 cgtcgttcgt gttgagcaga agccggacac tccgtacccg tttagagcgg gatgttgccg
     6781 tgcttcttcg gcgggttccg gtcgcgcttc gtatcgagcg cggccagcgc cgtgatgagc
     6841 tttcgacgcg tctgccgggg agggatgact tcgtcgatga acccgcgctg ggccgcgata
     6901 taggggctcg cgaacttctc gcgatactcc gccacgcgcg ccgcccgcgc cgcagcgacg
     6961 tcgccggccg cctcgagctc gcgcttatag aggatgttca cggccccttc cgcgcccatt
     7021 accgcaatct cggccgacgg ccacgcgaag ttggcgtcgg tgcggatgtg cttgctcgac
     7081 atcacgcaat aggcgcctcc gtacgccttg cgcgtaatca cggtcagctt gggcaccgtg
     7141 gcctcggcaa acgcgaacag gagcttggcg ccgtgacgga tgatcccgcc ccattcctga
     7201 accgtgccgg gaagaaaacc tggaacgtcc tcgaacgtca caagcgggat gttgaacgca
     7261 tcgcagaagc gcacgaaccg ggcggccttg accgatgcgt cgatgtccag ggtgcccgcc
     7321 agatgcgccg gttgattggc cacgaccccc acgggccggc cgccgagcct cgcgaagccg
     7381 acgacgatgt tcttcgcgaa gtgctcgtgc acctccagca gctcgcgatc gtcaactacg
     7441 gcgtggatga cgtcgagcat gtcgtacggc tggttggccg cggccggcac gatacggtcg
     7501 agggcctcgt cctcgcggtc cgcgggatcg gaaatctcgc gccgcggcgg gtcgtccacg
     7561 ttgttgccgg gcaggaacga caggagctcg cgaatcagca ggatcgcggc ctcgtcgtct
     7621 gcggcggcga agtgcgccac gccgctcttc tcgttgtgcg tcatcgcgcc gccgagctcc
     7681 tccttggtga cctcttcatg cgtcaccgtg cggatgacgt cggggcccgt cacgaacatg
     7741 tagctggttc ccttcaccat cacggtgaag tcggtgatcg cgggcgagta gacggctcct
     7801 ccggcgcacg ggcccatgat ggccgagatc tgcgggacga cacccgaggc gagcgtgttg
     7861 cggagaaaga tgtcggcgta gccgccgagc gagaccacgc cttcctggat ccgcgcgccg
     7921 ccggagtcgt tcaggccgat caccggcgcg cccatcttga cggccagatc catgatcttg
     7981 acgatcttgg ccgcgttggt ctctgacagc gagccgccga atacggt
//
LOCUS       DBNGSH010000660         2350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1257005, whole genome shotgun sequence.
ACCESSION   DBNGSH010000660 DBNGSH010000000
VERSION     DBNGSH010000660.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1257005"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1692
                     /locus_tag="AC16CY_15215"
     CDS             46..1692
                     /locus_tag="AC16CY_15215"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3355197.1"
                     /translation="MKLCTFLFDALWQRGVRHIFGIPGDFAHNRYEALLADGRFGLVT
                     LSHEPAVGFAADGSARITGGLGVCLITYGAGGLNMVNPVACAYAEQSPLVILSGGPGR
                     AERRAGIHVHHEVKSFESQFKVYQEVTEYCAILDDPKTAASHIRRALDVATTLKRPVY
                     LEIPRDMVAADILPPDDTPLERPTDSGAVEEAAGEIVSRLTRARSPVLIVGVEVHRFQ
                     LREAVIQLAETLGVPVASSFLGRGVFPTRHPQFVGTYLGLASPTELRDVVEASDCVLL
                     LGESISETSLGVSAQCLSEANLIIAVARDVFIGHHRYQNTPLASVISKLMTSPALTSV
                     PSRPRGSPGLPAAAPASPIPPDDDAPITRARVIDVINEFVQQHPDVPLVSDTGDCLFA
                     AVEIRSNEIVAPAYYATMGFAVPAALGIQVSSGRRPLVLVGDGAFQMTGPEIAHAPAY
                     NCNPVIVLLNNNRWEMLQAFFPHARYNETVAWPFARLAELWGGRGFEVLSARQFRDAL
                     AEAWRDERHALIEVSLAKGDISIVLRRFVDAMKARSAGAP"
     gene            1922..>2350
                     /locus_tag="AC16CY_15220"
     CDS             1922..>2350
                     /locus_tag="AC16CY_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HIR3355198.1"
                     /translation="MSRFSLRPTLSRAGIAVLVVAVVGLVGYGIGRTESPGAVASAAA
                     PAAPVASVQGASPSSYAAVVDAVAPAVVTVRVEKKASAMDTQLLDDDLFRFFGRELQI
                     PRQRAPRRSSGLGSGVITSPDGYVLTNNHVIDGADQVRVDL"
BASE COUNT          354 a          798 c          785 g          413 t
ORIGIN      
        1 gcgctgcatg gtctcgcgta atattcgaac gggccagtgt cgcacatgaa actctgtaca
       61 ttcctcttcg acgccctgtg gcagcggggc gtccgccaca tcttcgggat ccccggcgat
      121 ttcgcgcaca accggtacga ggcgcttctg gcggacgggc gattcggcct cgtcacgctc
      181 agccacgagc cggcggtcgg tttcgccgcc gacggcagcg cccgcatcac cggcgggctt
      241 ggcgtttgcc tgatcacgta cggcgccggc gggctcaaca tggtgaaccc ggtcgcctgc
      301 gcctacgctg agcagtcgcc gctcgtcatc ctgtccgggg gccctggacg cgcagagcgg
      361 cgcgcgggca tccacgtgca tcacgaggtc aagtcgttcg agtcgcagtt caaggtgtac
      421 caggaggtga ccgagtactg cgcgatcctc gacgacccga agaccgcggc gtcgcacatc
      481 cgccgcgcgc tcgacgtggc caccacgttg aagcggcccg tgtatctcga gattccccgt
      541 gacatggtgg cggccgacat ccttccgccg gacgacacgc cgctcgagcg tccgaccgac
      601 agcggcgccg tcgaggaagc ggcgggggag atcgtgtcgc gcctgacccg ggcgcgcagc
      661 ccggtgctga tcgtcggcgt cgaagtgcat cgcttccagc ttcgcgaggc cgtgattcaa
      721 cttgccgaaa cgctcggcgt gcccgtggcg tcgtccttcc tggggcgcgg ggtcttcccg
      781 acgcggcatc cgcagtttgt cggcacgtac cttggattgg cgtcaccgac ggaactgcgc
      841 gacgtcgtgg aggcctccga ctgcgtcctg ctgcttggcg agtcgatcag cgaaacgagc
      901 ttgggcgtat cggcgcagtg cctgagcgag gccaatctga tcatcgccgt ggcgcgggac
      961 gtgttcatcg gccatcaccg ctaccagaac accccgctcg cgtcggtgat ttcgaagctg
     1021 atgacgtctc cggcgctgac gtccgttccc agccggcccc gaggctcgcc gggcctcccc
     1081 gccgctgcgc ccgcgtcacc catcccgccc gacgatgacg cgccaatcac gagggccagg
     1141 gtcatcgacg tgatcaacga gttcgtgcag cagcaccccg acgtgccgct ggtgtcggac
     1201 acgggcgatt gtctttttgc cgccgtcgag atccgatcga acgagatcgt ggcgcccgcc
     1261 tactacgcca cgatggggtt tgccgtgccg gcggcgctgg gcatccaggt ctcgagcggc
     1321 cgccggccgc tcgtgctcgt cggcgacggc gcctttcaga tgaccgggcc cgagattgca
     1381 cacgccccgg cgtacaactg caatccggtc atcgtcctcc tgaacaacaa ccggtgggag
     1441 atgctgcagg cgttcttccc ccacgcccgg tacaacgaga cggtggcatg gccgttcgcc
     1501 aggcttgctg agctgtgggg aggacgcggc ttcgaggtgc tttcggcccg gcagttccgg
     1561 gacgcgctgg cggaggcgtg gcgggatgag cgccacgcgc tcatcgaggt cagcctggcg
     1621 aagggggaca tttcgatcgt gcttcgccgg ttcgtggacg ccatgaaggc gcgctccgcc
     1681 ggggcgccct gagaccgttc agcttcgtcc aaccagacgg ccggctggtg cgtccaactc
     1741 tttagtggac cgtatcaact ggaagagtac cgcgaacaac gagcttcgac cgcgattttc
     1801 cggccgcgac aacggagcgg ccggtagtaa ttcgcgaggg acataccact aagacaggaa
     1861 cccggggcgg tgggatggat gcgccgccgg tacccgttcg tacaaaagga gatctccagc
     1921 tatgtcacgc ttttccctgc gcccgacgct gtcgcgtgcg ggcatcgccg ttctcgtcgt
     1981 cgctgtcgtc ggtctcgtgg gatacggaat cgggcgaacc gaatcgcccg gcgccgttgc
     2041 gtctgccgcg gccccggcgg cacccgtggc ctccgtgcag ggggcgtcgc cctcgtccta
     2101 tgcggcggtc gtggacgcgg tcgctccagc cgtggtcacc gtccgtgtcg agaagaaagc
     2161 atccgcgatg gacacgcagc tcctcgacga cgacctgttt cgcttcttcg ggcgcgagct
     2221 tcagatcccc cgtcagcggg cgcctcggcg ctcttcgggg ctgggctccg gcgtcatcac
     2281 ctcgcccgac ggctacgtgc tgacgaacaa ccacgtcatc gacggcgccg atcaggtcag
     2341 ggtggatctg
//
LOCUS       DBNGSH010000661         3214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3465362, whole genome shotgun sequence.
ACCESSION   DBNGSH010000661 DBNGSH010000000
VERSION     DBNGSH010000661.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3465362"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC16CY_15225"
     CDS             complement(<1..240)
                     /locus_tag="AC16CY_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355199.1"
                     /translation="MALLMTFVIAVRALRRNAMRTALTALGMIIGVAAVIVMMALGSG
                     ARNAIETQIRNVGSNLVTVNAGSHRNWNVRGGQGGS"
     gene            complement(248..1015)
                     /locus_tag="AC16CY_15230"
     CDS             complement(248..1015)
                     /locus_tag="AC16CY_15230"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355200.1"
                     /translation="MGEPLITVRDLTRTYHVGEVDVRALRGVSLEVQPGEFIALTGPS
                     GSGKSTFMHLLGCLDTPTSGTYRLNGNDVAHLSKRELARVRNREIGFVFQGFNLLPRT
                     TALENVELPLLYARMPGKKRHTLAKAALESVGLGDRMHHHPNQMSGGQQQRVAIARAL
                     VTNPLLLLADEPTGNLDSRTSVEVMDIFQRLNVEKGLTIVLVTHEHDIAEYGTRIVAF
                     RDGRVRTDEPVKTRRAASVELEHLAAQADEADQILTA"
     gene            complement(1016..2722)
                     /locus_tag="AC16CY_15235"
     CDS             complement(1016..2722)
                     /locus_tag="AC16CY_15235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3355201.1"
                     /translation="MKKALIVVVTLLVLGGGGYYGYQVYKGPPPGMQLMTATVSRGSV
                     VQGIDATGRLQAVTTVQVGSQVSGTIKALYADFNTQVKKGQVIAELEPSLFITQVEQA
                     EASMVRIQADIERSKVQLEDSKLKLARARDLAKKQLLPQADLETAEANARSAEASLKS
                     AEAQLVQGNASLNQNKVNLSHTVIAAPIDGVVINRNVNVGQTVAASMQAPVIFEIAND
                     LSQMQVSANIDEADIGQVNPGQPVSFQVDAFPGQNFSGTVVQVRLNPVIEQNVVSYVT
                     VISAPNPEMRLRPGMTANVTVEVARVDDTLRVPTAALRFAPTPELFASLGQPVPESMT
                     ASAAPAGRGEDDAQAGGSRGGAAAGNREGRGGSGSGGFASGGEGRAGGEGRGGSDPQR
                     EALRAQLSQMSPEERQAFFAARGGGGGRGGFGGGGGGGFGQGGGGRTGGGGRGGEGRG
                     NARPGGRSGEGRRSVGQVWTVVDGKLTAVPVRVGITGGTNVAVSGQGLEEGMMLATGV
                     IETTVAATPLGGGNPLLPQLGRGRGGNFLNFGGGGNRGNQGGNRGGNQGGGNRGGGGR
                     GQ"
     gene            complement(2836..>3214)
                     /locus_tag="AC16CY_15240"
     CDS             complement(2836..>3214)
                     /locus_tag="AC16CY_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HIR3355202.1"
                     /translation="VTQKTVDALQTFAKTIYLSQTCQKKDGKTTWFLNLEDAFEAWYK
                     DPGPREWRTHFHVPVFLDDLGAFGTTRFALEQALAFHKKSPLSAQLEIETYTWDVLPD
                     HLKTGDIVDYVCRELDWVKGQLQ"
BASE COUNT          487 a         1123 c         1012 g          592 t
ORIGIN      
        1 cgacccgccc tgcccgcctc ggacgttcca gttccggtgg gagccggcgt tcaccgtcac
       61 gaggttggac ccgacgttgc ggatctgcgt ctcgatcgca ttccgcgcgc cgctgccgag
      121 cgccatcatg acgatgacgg cagccacgcc gatgatcatc cccagcgcgg tcagtgccgt
      181 ccgcatggcg ttgcgccgaa gggcccgaac ggcgatcacg aaggtcatga gcagagccat
      241 ggcgtcctca tgcagttagg atttggtcgg cctcgtcggc ctgagcggcg aggtgttcca
      301 gttcgaccga cgccgcgcgc cgggtcttga ccggctcgtc ggtgcgaacg cgcccgtcgc
      361 gaaacgccac gatccgcgtc ccgtattccg cgatgtcgtg ctcgtgggtc acgagcacga
      421 tcgtcagccc cttctcgacg ttcaggcgct ggaagatatc catcacttcg acgctcgtgc
      481 gcgagtcgag attgcccgtc ggctcgtcgg cgagcagcag gagcggattc gtgaccagcg
      541 cgcgcgcgat tgccacgcgc tgctgctggc cgccggacat ctgattggga tgatgatgca
      601 tacggtcgcc cagcccgacc gattcgagcg cggccttcgc gagcgtgtgc cgcttcttgc
      661 cgggcatgcg cgcgtagagc aacgggagct cgacgttctc gagagcggtc gttcttggca
      721 gcaggttgaa tccctggaag acgaacccga tctctctgtt ccgcacgcgc gccagctcgc
      781 gcttcgagag atgcgccacg tcgttgccgt tgaggcgata tgttccggag gtgggcgtat
      841 cgagacagcc gagcaggtgc atgaaagtgg actttcccga accggacggg ccggtcaagg
      901 cgatgaactc ccccggctgt acctcgagcg agacgccgcg aagcgcgcgg acgtccactt
      961 cgccaacgtg atacgtcctc gtgagatcgc ggacggtgat gagcggctcg cccatttatt
     1021 ggccgcgtcc tccgccgccc cggttcccgc cgccctggtt cccgccgcgg ttcccgccct
     1081 ggtttccgcg attcccgccg ccgccgaagt tgaggaagtt gccgcctcgt ccgcgaccga
     1141 gctgcggcag cagcgggttg ccgccgccaa gcggcgtcgc cgccacggtc gtctcgatga
     1201 cgcccgtcgc gagcatcatg ccttcttcga ggccctgtcc cgacacggcg acgttggttc
     1261 caccggtgat cccgactcga accgggactg cggtcagctt tccatcgacc accgtccaga
     1321 cctgtcctac ggagcgccgc ccctcgcccg aacgcccgcc cggtcgagcg ttgccccggc
     1381 cctctccgcc gcgtccgccg ccaccagtgc gtccgccgcc gccctggccg aacccgccgc
     1441 caccgccacc accaaacccg ccgcggccac cgccgccgcc gcgcgcggca aagaacgcct
     1501 gccgctcttc cggcgacatc tgcgacaact gtgcgcgaag agcctcgcgc tgtgggtcgc
     1561 tcccgccgcg accctcgccg cccgcgcgac cctcgccgcc ggaggcgaag ccgccgctgc
     1621 ccgagccgcc gcgtccttca cggttccccg ccgccgcgcc gcctcgagag ccgccggcct
     1681 gagcgtcgtc ctccccgcgg cccgccggtg cggcggacgc cgtcattgac tccggcaccg
     1741 gctgaccgag cgaagcaaac agctccggcg tgggcgcaaa gcgcagggcg gccgtcggca
     1801 cgcgaagcgt gtcgtcgaca cgcgcaactt cgacggtgac attggccgtc atccccggcc
     1861 tcaaccgcat ttccgggttg ggtgcgctga tgaccgtgac gtagctcacg acgttctgct
     1921 cgatcacggg gttgagccgc acctggacca cggtgccaga gaagttctgc cccgggaatg
     1981 catcgacctg gaagctcacc ggctggccgg ggttgacctg gccgatgtcc gcctcgtcga
     2041 tgttggcgct cacctgcatc tgcgacagat cgttggcgat ctcgaagatc accggagcct
     2101 gcatgctggc ggccacggtc tggccgacgt tgacgttgcg gttgatgacc acgccgtcga
     2161 tcggcgccgc gatgacagtg tgactgagat tgaccttgtt ctgattgagc gaggcgttgc
     2221 cctgcacgag ctgcgcttcc gcggacttga gcgaggcctc ggcgctccgg gcgttggcct
     2281 ccgccgtctc gagatcggcc tgcggcagaa gttgtttctt cgccagatcg cgcgcgcgcg
     2341 ccagcttcag tttcgagtct tcgagctgga ccttggaccg ttcgatgtcg gcctggatgc
     2401 gaaccatgct ggcctcggcc tgttcgacct gggtgatgaa cagcgacggc tcgagctcgg
     2461 cgatgacctg gcctttcttg acctgtgtat tgaagtcggc gtagagcgcc ttgatcgtcc
     2521 ccgacacctg cgacccgacc tgcaccgtcg tcacggcctg caaccggccg gtcgcgtcga
     2581 tgccctgcac gacggatccg cggctcactg tcgccgtcat gagctgcatc ccgggcgggg
     2641 gccccttata gacctggtac ccgtagtatc cgcccccccc gaggacaagc agggttacga
     2701 cgacaatcag agcttttttc ataccttacc tagtacatac tgcgcggccg ccccagatct
     2761 ttaagtggag gttaaataaa gcccgacttc cccactttcc ccttccggct gttgactttc
     2821 cgggttattg ccaagctatt ggagttgccc cttcacccag tcgagctccc ggcacacgta
     2881 gtccacgata tcgccggtct tgagatggtc aggtagtaca tcccacgtgt aggtctcgat
     2941 ttcgagctgg gccgaaaggg gtgatttctt gtgaaaggcc aacgcctgct cgagcgcgaa
     3001 ccgtgtggtg ccgaaggcgc ccaggtcgtc gagaaagacg ggcacgtgaa agtgcgtccg
     3061 ccactcgcgc gggccggggt ccttgtacca ggcctcgaag gcatcttcga gattcaggaa
     3121 ccaggtcgtc ttgccgtcct tcttctggca ggtctgggag aggtagatcg tcttcgcgaa
     3181 ggtctgcagc gcatccacgg tcttctgcgt cacc
//
LOCUS       DBNGSH010000662         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4654569, whole genome shotgun sequence.
ACCESSION   DBNGSH010000662 DBNGSH010000000
VERSION     DBNGSH010000662.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4654569"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="AC16CY_15245"
     CDS             <1..178
                     /locus_tag="AC16CY_15245"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3355203.1"
                     /translation="AHNPENEEFGEERLQQLLRQIAHLPADEISARISAEMRNWIRDA
                     EQYDDLTFIVMKVR"
     gene            228..>2830
                     /locus_tag="AC16CY_15250"
     CDS             228..>2830
                     /locus_tag="AC16CY_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3355204.1"
                     /translation="MRVLFSMRHTGVLRNFASTLQALAARNHQVHLVFGHQDKEGDNR
                     LLQELLSAPNITCRDVPKKTPWRFWLGLARAARYSVDYVRYLTPEYEGVNSLKERARG
                     KAPAPMRWFVERPIFRSRAGNRFLTGALLAVERAIPIDRAVLAAVERERPDVLLVSPL
                     VDIGSDQVDYVKAARRLGIRSALPVLSWDNLTNKGLIRLQPGRVYVWNEAQKTEAVDL
                     HGSHPDRVVVTGAMVYDQWFARRPSSTRGEFCARVGLDPARPILLYLCSSPFIAPDEL
                     TFIESWIAAVRAAPDARVSTAGILVRPHPENRQPWHRFDELSFSNVAIWPKGGASPVD
                     AESRNDFFDSMYHAAAAVGINTSAQIECGIVGRPVFSVRTPAYQRTQDGTLHFRHLTS
                     EGGGLLHVADDFDSHVRQIAAALDDPDGTTRRIHGFVKAFTRPHGLDVEATPRMVDAI
                     EQFAATPPPAPERLPLYVYPVRTLLYPVAVLLAAVRRVTRVSRKRQRQLRPITITGTI
                     LKVTLALFDWLFRFRPIRNFAKRHIVPRALSRLSAVDAPSEEAVAVPRVLHRISRRDR
                     PIIVGPWVSEVGYELLYWIPFLQWVTSRRLLDADRLVVVSRGGCAAWYRDVTARYVDL
                     FDYYTPEQFRQRSEQRATDGRQKPRTMTEFDRNTIKLVRQGAHLAHCDLLHPMYMYRL
                     FHGFWQSRAAVNAVENFTQFKPLRVESGHLAGRLPDDYVAVRFYFNDVFPDTDTNRRF
                     VADLLNGLAETTDVVLLNPATELDDHRDVPVAARGRIHSIAHLMSPRSNLELQSAVVS
                     RARAFVGTHGGLSYLAPLLGVKSLSFYSETRPFTLRHLELARRVFTGGPFGSYVALDV
                     NDLDTLRATHV"
BASE COUNT          457 a          977 c          918 g          478 t
ORIGIN      
        1 ggcgcacaac cccgagaacg aagagttcgg cgaggagcgg ctgcagcaac tgctccgcca
       61 gatcgctcac ttgccagccg acgagatcag cgcgcggatt tccgctgaaa tgagaaactg
      121 gattcgcgac gcggagcagt acgacgatct cacgttcatc gtgatgaagg tgcgctgagg
      181 gtccttcggc gctgtcgaga cgcgagtggt attttagaga cgcataaatg cgagtcctgt
      241 tttcgatgcg gcacaccgga gtgttgagga atttcgcgtc gacgctccag gcgctggccg
      301 cgcggaatca ccaggtccac ctcgtcttcg gccaccagga caaagagggc gacaaccggc
      361 tgctccagga gctgctgtcc gctcccaaca tcacctgcag ggacgtcccg aagaagactc
      421 cctggcggtt ctggctcggc ctggcgcgcg cggcgcggta cagcgtggac tacgtccgct
      481 acctgacgcc cgaatacgaa ggcgtcaaca gcctcaagga gcgcgcacga ggcaaggcgc
      541 cggcgccgat gcgctggttc gtggagcggc cgatcttccg cagccgcgcc gggaaccggt
      601 ttctgaccgg ggccctgctc gccgtcgaac gcgccattcc catcgaccgg gccgtcctgg
      661 ccgccgtcga gcgcgagcgg cccgatgtgc tcctcgtcag ccctctcgtg gacatcgggt
      721 ccgatcaggt cgactacgtc aaggccgccc ggcgcctggg catccggtcg gcgttgcccg
      781 tcctcagctg ggacaacctc acgaacaagg ggctgatcag gctgcagccc ggccgcgtct
      841 acgtctggaa cgaggcgcag aagacggaag ccgtggacct gcacgggagc catccggacc
      901 gcgtggtcgt caccggcgcg atggtgtacg accagtggtt cgcgcgcagg ccgagctcga
      961 cgcgcggcga gttctgcgcc cgcgtcggcc tcgacccggc cagaccgatt ctcctctacc
     1021 tctgctcgtc gcccttcatc gcgccggacg aactgacgtt catcgagtcc tggatcgccg
     1081 ccgtccgcgc ggcaccggac gcgcgcgtgt ccacagcggg cattctcgtg cgcccgcatc
     1141 ctgaaaaccg ccagccgtgg caccggttcg acgagctgtc gttttcgaac gtggccatct
     1201 ggccaaaggg cggcgccagc ccggtggacg ccgaatcgag aaacgacttc ttcgactcga
     1261 tgtatcacgc cgcggcggcc gtcggcatca acacgagcgc ccaaatcgaa tgcggcatcg
     1321 tagggcgccc ggtgttttcg gtcaggacgc cggcctacca gcgcacccag gacggcacgc
     1381 tgcatttccg gcacctgacg tcggaaggcg gcgggctgct gcacgtcgcc gacgatttcg
     1441 actcgcacgt gcgtcagatc gcggccgcac tcgacgatcc ggacggcacg acgcggcgga
     1501 ttcacgggtt cgtcaaggca tttacgcggc cgcacgggct cgacgtcgag gcgacgccgc
     1561 gcatggtcga tgcgatcgag cagttcgccg ccacgccgcc gcccgcgccc gagcggctgc
     1621 cgctctacgt gtaccccgtg cggaccctgc tctaccctgt cgccgttctg ctggcggctg
     1681 ttcgccgcgt cacgcgcgtg tcgcgcaagc ggcagcggca gctgcggccc atcacgatca
     1741 ccggaacgat cctcaaggtc acgctggcgc tcttcgattg gctgttccgg ttccgtccga
     1801 tccggaactt cgcgaagcgg cacatcgttc cgcgcgcgtt gtcgcgtctg tccgcggtcg
     1861 atgcgccgag cgaggaagcc gtcgccgtgc cgcgggtgct gcatcggatc tcacgcaggg
     1921 atcgccccat catcgtcggc ccatgggtga gcgaagtcgg ctacgagctc ctgtactgga
     1981 tcccgtttct ccagtgggtg acgagccggc gcctgctgga tgccgatcgc ctcgtcgtcg
     2041 tctcgagggg cggctgcgcg gcctggtacc gagacgtcac ggcacggtac gtcgacctct
     2101 tcgactacta cacgccggaa cagtttcgac agcgcagcga gcagcgcgcg accgacggca
     2161 ggcagaagcc gcggacgatg acggagttcg atcgcaacac gatcaagctc gtgcggcagg
     2221 gagcgcatct ggctcactgc gacctgctgc acccgatgta catgtaccgg ctgttccacg
     2281 gattctggca gagccgggcc gcggtcaacg ccgtggagaa cttcacgcaa ttcaagccgc
     2341 tgcgcgtcga gagcggccat ctggccgggc ggctcccgga cgactacgtg gccgtccgtt
     2401 tctacttcaa cgacgtgttc cccgataccg acacgaaccg ccgattcgtc gccgatctcc
     2461 tgaacggcct cgccgagaca acagacgtcg tcctgctgaa tccggcgacg gagctcgacg
     2521 atcatcgcga tgtgcccgtg gcggctcgcg gacgcatcca ttccatcgcg cacctgatgt
     2581 cgccgcgctc gaatctcgag ctgcagtcgg cggtcgtctc gcgggctcgc gcattcgtcg
     2641 gcacacacgg cggcctgtcg tatctggcgc ccctgctggg cgtcaagtcg ctttcgttct
     2701 actcggagac gcgcccgttc acgctgcggc atctcgagct ggcgcggcgc gtcttcacgg
     2761 gcgggccgtt cgggtcgtac gtggcgctcg acgtcaacga tctcgacacg ctgcgcgcaa
     2821 cccacgttca
//
LOCUS       DBNGSH010000663         4810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2786425, whole genome shotgun sequence.
ACCESSION   DBNGSH010000663 DBNGSH010000000
VERSION     DBNGSH010000663.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2786425"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="AC16CY_15255"
     CDS             <1..1092
                     /locus_tag="AC16CY_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3355205.1"
                     /translation="FDPLHHGHVEYFSRARQVGTPLLCNIAPDFYTSAKHPPLLPAQN
                     RAIVIDALKPIDYTHVSPVDTETVLRELQPTHYIKGNDWEGKLPPEQVKICEKEGIQI
                     VYLDTVRDSSTQLLKAFQTGHNGKADVTAFENLVFSQKRVEATHYDQDYWTAEWRQAG
                     NVYTVEKRREIEGRHPLVIKEVFTPARVLDVGCGPGILMYLLHEVGVVADGVDPAPGM
                     REMAPPEVRDRIFMGGANTPDIPSNRYDLVICREVFEHMTVLEVRDAVRNICRMSSRF
                     AYATTRFHQNPHSLLDVSTQDELDPSHITMMNKDLLRLFFVLEGFRQRKDLEDRIDWM
                     GKGRVLVYEKWAGDRGQGAAGQGAGEETR"
     gene            1089..2174
                     /locus_tag="AC16CY_15260"
     CDS             1089..2174
                     /locus_tag="AC16CY_15260"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR3355206.1"
                     /translation="MTRQIIIANRVINDDSEAYVIAEIGHNHQGSLKTAKELFQSAAE
                     CGVAAAKLQKRDNRSLYTREMFDKPYDNENSFGATYGEHREALEFGKSEYEELQAEAD
                     RLGLAFFSTAFDMKSADFLAQLGAQAYKIASGDLKNTPLLKHVAKLGKPMIVSTGGGT
                     LDDVQRAYDTVMPINPRLCLLQCTCGYPAEFAELDLRVIATYRERFPEVVIGYSGHDN
                     GIAMPLAAYMLGARVIEKHFTLNRAMRGTDHRFSLEPVGMRKMIRDLQRTRMALGDGR
                     KKVYASEASPVLKMGKKLVAARDLPAGHVLSVADIAIKSPGDGLAPYYLDQIVGHSLT
                     RDLRADETLTLDLLQPASGAGRSTARV"
     gene            2171..2983
                     /locus_tag="AC16CY_15265"
     CDS             2171..2983
                     /locus_tag="AC16CY_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3355207.1"
                     /translation="MSGRFGLDGRIAVVTGALGNLGPVWIEGLLEAGATVAGLDLPGA
                     APNAAFARMQAAFGETRLALFGADVTDRAAMLSARDEIAARFGAPHVLVNNAGIDSPP
                     SASGGGARLEDIPLDVNRRIFEVNTLGLFQATQVFGAGMVAARRGSIINIGSLYASVA
                     PDPRFYDHIKSDPPFLKPPAYGASKAAVVNLTKYLATAWGPYGVRVNALSPGGVLGGQ
                     DEPFKRKFGDRVPLGRMAVYEDLIGPLQFLASDASAYVTGIELRVDGGFTAW"
     gene            3017..4174
                     /locus_tag="AC16CY_15270"
     CDS             3017..4174
                     /locus_tag="AC16CY_15270"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR3355208.1"
                     /translation="MSVDSSGARRPLAVRAGLVVMNGSMKVVRHGTRIRSYVRRFVRF
                     VLQGLRVVRHALLRVRQSWKGFRYRSRWTRWGGAAWRTAAFVKAPRETRERRLLAEAY
                     TSKLTFAELSKDRGFRLLPPDHFGDMAGVIATCRRLFESKVARRDAVAGSRGDAPDGH
                     LRVRETKGEYLRNLLSNDDLQNHPALVDFALSDAAMGMATSYLGTIPYLNRVDLLYSV
                     PRPGDEKVSSQLFHVDPEGLTQVKFFINVFDVDEAEGPFTFIPADETARVIREVGALR
                     RKLGKQHAGRYLDDEIAAVGATDAIVRVKGPQGSGVAVDTSRCLHLGSRVAPGTFRLC
                     LYVQYCTTRERGNAFDVERFRNDPVRHLAIRHSLASRGKGVSAPHQMGGMS"
     gene            4177..>4810
                     /locus_tag="AC16CY_15275"
     CDS             4177..>4810
                     /locus_tag="AC16CY_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355209.1"
                     /translation="MRILVHVTLTTMLRHFESVLLSLADRGHTVRIASPVRKSKAPPL
                     PALEAHRNISFITAPDGRSDHWKDRIADLRVLRDYLRYLEPRFAHSPKLRSRAARRMA
                     HVTTRGEWGHLVARCPRCHERLIDDEVGRMLHGFRHAGLTSLAALLASTEELVPSDSG
                     LEAFLREQQPDVLLITPLIMSGSQQPDFVKSAKALGVPVVFPVFSWDNLST"
BASE COUNT          839 a         1607 c         1604 g          760 t
ORIGIN      
        1 ttcgatccgc tccatcacgg gcacgtcgag tatttcagca gggcgcggca ggtcggcacg
       61 ccgctgctct gcaacatcgc gcccgatttc tacacgagcg cgaagcaccc gccgctgctt
      121 ccggcccaga acagggccat cgtgatcgac gcgttgaagc cgatcgacta cacgcacgtc
      181 agcccggtgg acacggagac agtcctgcgc gagctccagc ccactcacta catcaagggg
      241 aacgactggg agggcaagct gcccccggag caggtgaaga tctgcgagaa ggaaggcatt
      301 cagatcgtct atctcgacac ggtgcgtgac tcatcgacgc agctcctcaa ggcattccag
      361 accgggcaca acggcaaggc cgacgtcacg gctttcgaga acttggtgtt ctcgcagaag
      421 cgcgtcgagg ctacgcacta cgaccaggac tactggaccg cggagtggcg gcaggctggc
      481 aacgtctaca ctgtcgagaa gcgccgcgag atcgaagggc gccacccgct cgtcatcaag
      541 gaagtgttca ccccggcacg cgtgctcgac gtcgggtgcg gccccggcat cctgatgtat
      601 ctgctgcacg aggtcggcgt cgtggccgac ggtgtcgatc cggcgccggg catgcgcgag
      661 atggcgccgc ccgaagtgcg cgatcgcatc ttcatgggag gcgcgaatac cccggacatc
      721 ccgtcgaacc gctacgacct ggtgatctgc cgggaggtgt tcgagcacat gaccgtgctc
      781 gaggtgcggg acgcggtgcg caacatctgc cgcatgtcat cccgcttcgc ctacgcgacg
      841 acacggttcc atcagaatcc gcattcgctg ctcgacgtga gcacgcaaga cgagctcgac
      901 ccgtcgcaca tcacgatgat gaacaaggat ctgctccggc tgttcttcgt gctggaagga
      961 ttccgtcagc ggaaagacct ggaagaccgc atcgactgga tggggaaggg gcgggtgttg
     1021 gtgtacgaga agtgggcagg ggacaggggg cagggggcag cggggcaggg ggcaggggag
     1081 gagaccagat gacccggcag atcatcatcg ccaatcgcgt catcaacgac gacagcgagg
     1141 catacgtcat cgccgagatc ggccataacc accagggcag cctgaagacg gccaaggagc
     1201 tgtttcaatc ggcggccgag tgtggtgtgg cggcggccaa gctgcagaag cgcgacaacc
     1261 ggtcgctcta tacgcgggag atgttcgaca agccgtacga caacgagaac agcttcgggg
     1321 cgacctacgg cgagcaccgc gaagcgctgg agttcggcaa gagcgagtac gaggagctcc
     1381 aggcggaggc cgaccggctt ggccttgcgt tcttctccac ggcgttcgac atgaagagcg
     1441 ccgatttcct ggcccagctc ggcgcccagg cgtacaagat cgcgtccggc gatctcaaga
     1501 acacgccgct cctgaagcac gtcgccaagc tcggcaagcc gatgatcgtc agcaccggcg
     1561 gcgggaccct cgacgacgtc cagcgcgcgt acgacacggt catgccgatc aacccgcggc
     1621 tctgtctact gcaatgtacc tgcgggtacc cggcggagtt cgccgagctc gacctccgcg
     1681 tcatcgccac gtaccgcgag cggtttccgg aagtcgtcat cggctactcg gggcacgaca
     1741 acgggatcgc gatgccgctt gccgcctaca tgctcggggc acgcgtgatc gagaagcact
     1801 tcacgctgaa ccgggcgatg cgcggcaccg accacaggtt ctcgctcgag ccggtcggca
     1861 tgcggaagat gattcgcgac ctgcagcgca cgcgcatggc gctcggcgac ggccggaaga
     1921 aggtctacgc gagcgaggcc agcccggtcc tcaagatggg caagaagctc gtggcggcgc
     1981 gggatctgcc tgcgggccac gtcctctccg tggccgacat cgcgatcaag agcccgggag
     2041 acgggctcgc gccgtactac ctggatcaga tcgtcggcca cagcctgacc cgcgacctgc
     2101 gagccgacga gaccctcacg ctcgacctgc tccagccggc ctccggcgcg ggccgctcga
     2161 ctgcgcgcgt gtgagcggcc gcttcggact cgacggccgg attgcagtcg tcaccggcgc
     2221 gctcggcaac ctcgggccgg tgtggatcga agggctgctc gaggccggag ccaccgtcgc
     2281 cgggctcgac ctgcccggtg ccgcacccaa cgcggcgttc gcgcggatgc aggcggcctt
     2341 cggcgagacc aggctggcgc tcttcggcgc cgacgtgacc gatcgcgccg cgatgctgtc
     2401 ggcccgcgac gagattgcgg cccgcttcgg cgcccctcac gtgctcgtca acaacgccgg
     2461 catcgattcg ccgccgtcgg cctcgggcgg aggcgcccgc ctcgaggaca tcccgctcga
     2521 cgtcaatcgc cgcatcttcg aggtcaatac gctggggctc ttccaggcga ctcaggtctt
     2581 cggcgccggg atggtcgccg cgcgcagggg ctcgatcatc aacatcggct cgctctacgc
     2641 ctcggtcgcg cccgatccgc gcttctacga tcacatcaag agcgacccgc ccttcctgaa
     2701 gccgcctgcc tacggcgcct cgaaagcggc cgtcgtgaac ctgacgaagt atctggccac
     2761 ggcgtgggga ccctatggcg tgcgcgtgaa cgcgctgtcg ccaggcggcg tcctgggcgg
     2821 ccaggacgag ccgttcaagc ggaagttcgg cgaccgcgtt cccctgggcc gcatggcggt
     2881 gtatgaggac ctcatcggac cgctgcaatt cctcgcctcc gacgcctcgg cctacgtcac
     2941 ggggatcgag ctgcgcgtcg atggaggctt cacagcgtgg taggcaggtg gtcgtgacga
     3001 caggagcgag ccggccatga gcgtggactc gagcggcgcc cgcagaccgc tcgccgtacg
     3061 ggcgggcctc gtcgtgatga acggatcgat gaaagtcgtt cgtcacggga cacgcatccg
     3121 cagttacgtg cgccgcttcg tgcggttcgt cttgcagggg ctgcgggtcg ttcggcacgc
     3181 gttgctccgc gtcaggcagt cgtggaaggg tttccgctac cggagccgat ggacccgatg
     3241 gggcggcgct gcgtggagaa ccgcggcgtt cgtgaaggcg ccgcgcgaga cgcgcgagcg
     3301 gcgccttctc gcggaggcct acacgtcaaa gctgacgttc gccgagttgt cgaaagatcg
     3361 ggggttccgc ctgctgccgc cggatcactt cggcgacatg gccggcgtca tcgccacgtg
     3421 ccggcggctg ttcgagagca aggtggcccg ccgcgatgcc gttgccggct cgcgaggcga
     3481 cgcgccggac gggcatctcc gcgtccgcga gacgaagggc gagtacctcc gcaacctgct
     3541 gagcaacgac gacctgcaga atcatcccgc gctggttgac ttcgcactga gcgacgcggc
     3601 catgggcatg gccaccagct acctcggcac catcccgtat ctcaaccggg tagacctcct
     3661 ctactcggtg ccccgaccgg gcgacgagaa ggtgtcgagc cagttgtttc acgtcgatcc
     3721 ggaagggctg acgcaggtca agttcttcat caacgtcttc gacgtggacg aagccgaggg
     3781 tccgttcacc ttcatcccgg cggacgagac ggcacgcgtg atccgcgagg tcggtgcgct
     3841 ccggcgaaag ctcggcaagc agcacgccgg acgctacctg gacgacgaga tcgccgccgt
     3901 gggcgcgacc gacgccatcg tccgcgtcaa aggacctcag gggtcggggg ttgccgtcga
     3961 tacgtcgcgc tgcctccatc tgggcagtcg agttgcgccg ggaacgttcc gcctgtgcct
     4021 ctacgttcag tactgcacga cgcgcgagcg tggaaacgcc ttcgacgtcg agcggttccg
     4081 gaacgatccc gttcgtcacc tcgccatccg gcactccctc gcttcaagag ggaaaggcgt
     4141 gtcggcgccg caccagatgg gcggcatgtc ttaagcgtgc gcattctcgt ccacgtcacc
     4201 ctcaccacga tgctccggca cttcgagtcg gtgctgctgt cgctcgccga ccgggggcac
     4261 acggtgcgga tcgcctcgcc cgtccgaaaa tcgaaggccc cgcctctccc ggcgctggaa
     4321 gcgcaccgga acatctcgtt catcaccgcc ccggacggcc ggagcgatca ctggaaggac
     4381 cggatcgccg atctgcgcgt gctcagggat tacctgcggt acctcgaacc gcgctttgcc
     4441 cactctccga agctgcgaag cagagccgcc cgcaggatgg cccacgtgac gacacgcggc
     4501 gaatgggggc acctggtggc gcgctgccca cgttgtcacg agcgcctcat cgacgacgaa
     4561 gtggggcgca tgctccacgg gtttcggcac gcggggctga cgagtctcgc ggccctgctc
     4621 gcgtctaccg aagagctcgt gccgagcgac agcggcctcg aggcgttcct gcgagagcag
     4681 caacccgacg tcctgctgat cacgcccctc atcatgagcg gttcgcaaca gccggatttc
     4741 gtcaagagcg ccaaagcgct cggcgtgccg gtcgtgtttc ccgtgttcag ctgggacaac
     4801 ctgtccacca
//
LOCUS       DBNGSH010000664         5374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3635408, whole genome shotgun sequence.
ACCESSION   DBNGSH010000664 DBNGSH010000000
VERSION     DBNGSH010000664.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3635408"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="AC16CY_15280"
     CDS             <1..718
                     /locus_tag="AC16CY_15280"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355210.1"
                     /translation="MPFRVIGVQKEKIQLSNYNRPDKESVFIPYTTAGQLWNTEFLSV
                     LIYQAVDPTFDARTTAQVKETLGRRLRFNPADERALNTFGSAEAQKITSGIVLGLKLV
                     LTFIGVLTLAIGGVGVMNIMFVSVTERTREIGLRKAVGARRATVLAQFMLEGLVTTFA
                     GGAAGVLISWVLVWIFSPRPFLSELLDDATRSGDIYLLLSLELVGICTAILMVVGLIS
                     SFIPALRASRLDPIEALRYE"
     gene            821..1024
                     /locus_tag="AC16CY_15285"
     CDS             821..1024
                     /locus_tag="AC16CY_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355211.1"
                     /translation="MAHRDDELNGPSGDDARPVLFVQWWAVVTEPFLLLRERLRGERC
                     PNCHARVRDEHGRAVNAAPICSP"
     gene            1021..1500
                     /locus_tag="AC16CY_15290"
     CDS             1021..1500
                     /locus_tag="AC16CY_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355212.1"
                     /translation="MISRPRRQPVTLEAVGAIAILVAVAASYFFLYTWPSIAPLVYQV
                     APGFRATKTGPEGFPVVVRINQSSLRITNGSEVRWTCAVWLPPAPVFSAGVRLAPTES
                     RDLPYESFTGQSGTLTDGTGYTGRGGRLKSTASRTRGAGIAPDSFERSQRSLLTPDC"
     gene            1580..2113
                     /locus_tag="AC16CY_15295"
     CDS             1580..2113
                     /locus_tag="AC16CY_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355213.1"
                     /translation="MTLAPRIEGTLPIRSPAAAFLQAFSRRAESGLLTGRPQPRSSYA
                     ATVVSDGGLRIRAADWSTAISVGLNDLELRPAHDGAVRYRLQYWRWTCYAVGLSGVLG
                     GIGLWLLLAFDAREYIANHPDSQLPGLSIDQNLLMAWAMVLFWGFAWPWILVALHKRP
                     LRRLIARIAAEVDASVA"
     gene            complement(2206..3285)
                     /locus_tag="AC16CY_15300"
     CDS             complement(2206..3285)
                     /locus_tag="AC16CY_15300"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HIR3355214.1"
                     /translation="MTTYKIAVLAGDGIGKEVTPAGIEVLKLAAEQGGFRCEFTTLPW
                     GCDYYLQHGRMMDADGIDQLLKFDAIYMGAIGDPRVADHISARELILPLRQQLGQWVN
                     LRPMRLLPGISSPLAGRRQSDIDMICVRENSEGEYCGIGGRLHRGTPQEVAEQTGVFT
                     RHGIERIARYAFRLAASRPRKMLASATKSNALQHAMVMWDEVVAGVAKEFPDVELKKY
                     HVDALCARMVTHPHTLDVIVASNLFGDILTDIGSAISGSLGVAPGGNINPDKTTPSMF
                     EPIHGSAPDIAGKGIANPIAAIWAGAMMLDHLGQRASHDRILAAIERVLSDERVKTPD
                     LGGRATTREMTTAVTAAMSASRQPA"
     gene            complement(3300..4691)
                     /locus_tag="AC16CY_15305"
     CDS             complement(3300..4691)
                     /locus_tag="AC16CY_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3355215.1"
                     /translation="MSPHLVLIVVYSLTVVGVGIWTARFVRTSSAFFVAGRSLGPGLV
                     FASMLAANIGAGATVNVAGLAYREGLSAWWWSGSAGLASLVLAFWVGPRLWTLAKQYG
                     FYTTGDFLEHRYGPAVRGIVTGLVAFGSLWILAAQLIAGAAILNVVTGAPRWAGSLVG
                     GAIMTIYFTAGGLLGTAWVNSLQLVVMLVGFILAAAFVIAGAGGVDALAYGMPATYAD
                     ITYSSGPGSGWTLLALTGPAFVISPGLIQKSYGAASARALRIGVALNGVALMLFAFLP
                     VMLGMAGRAALPEVSSPDLVLPMVLTQLLPAWLGALALAAVFSTEVDTCDAILFMIST
                     SASKDLYQRYVKPSATDAELLRVARLSAVSGGALGVLLSIYLSTIVQAMTVFYSLLGV
                     SLFVPVLGGLWSRRAGQPEALGAIAAGVVTLLVVRFGFAGRYPWLDPTLAGLAAAGIA
                     FAVMLIARRDRRR"
     gene            complement(4688..>5374)
                     /locus_tag="AC16CY_15310"
     CDS             complement(4688..>5374)
                     /locus_tag="AC16CY_15310"
                     /inference="COORDINATES: protein motif:HMM:NF015535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR3355216.1"
                     /translation="AAVLMDLGFGGSSSIRPTADCGYRAAMAASNGPVAEGNVGAGAG
                     ATVGKIAGSRARAMKAGVGSASIALPGGLVVGAIVAVNAVGDVVDPATGAVVAGVRTA
                     DGKRLADARVLLRSPAATGGPRPVENTTIAVVATNARLTKAEAGRVALMADDGLARAI
                     YPAHTPADGDTVFALATGSWQGRSDVTAIGALAADALSAAIVRAAVQATSSGGLPAAR
                     DLGTVPQRFR"
BASE COUNT          891 a         1821 c         1778 g          884 t
ORIGIN      
        1 aatgccgttc cgggtgatcg gcgtgcagaa ggagaagatt cagctttcga actacaaccg
       61 gcccgacaag gaaagcgtgt tcattcccta cacgacggcc gggcagctct ggaacacgga
      121 gtttctcagc gtcctcatct accaggccgt cgatccgacg ttcgatgccc gcacgacggc
      181 gcaggtgaag gaaacgcttg gcaggcggct gcggttcaac ccggccgacg agcgggcgct
      241 gaacacgttc ggatcggcgg aggcgcagaa gatcacgagc ggcatcgtgc tcgggctcaa
      301 gctcgtgctg accttcatcg gcgtgctgac gctcgccatc ggcggcgtgg gcgtcatgaa
      361 catcatgttt gtgtcggtga ccgagcgtac gcgcgagatc gggcttcgca aggcggtggg
      421 ggcgagacgc gccaccgtgc tcgcgcagtt catgctcgag ggcctcgtga cgacgttcgc
      481 cggcggcgcc gccggcgtcc tgatctcctg ggtgctcgtc tggatcttca gcccgcgccc
      541 gttcctgtcc gagctgctcg acgatgcgac acgctccggc gacatttacc tgctgctgtc
      601 gctcgaactg gtgggcatct gcacagccat cctgatggtc gtcggcctga tcagctcctt
      661 catcccggcc ctgcgcgcgt cgcggctcga tccgatcgag gccctgcggt atgagtgacg
      721 caatccattg gtggttcttg atctgtgatt gttgaaacgt tgttgattgt tgatttctga
      781 tcggtgattg atcgacgatt tcggtgccgg gagagtcacc atggcgcatc gcgacgacga
      841 actgaacggc ccgtcgggcg acgacgcccg accggtgttg ttcgtccagt ggtgggcggt
      901 cgtgaccgag cccttcctgc tcctgcgaga acgtctgcga ggggagcggt gcccgaactg
      961 ccacgcccgg gtacgcgacg agcacggccg tgcggtcaat gcggcaccga tctgcagccc
     1021 ttgatttcga ggccgcggag gcagcccgtc acgctcgagg cggttggagc gatcgcgatt
     1081 ctcgtggccg tggccgcctc gtacttcttt ctctacacct ggccctcgat cgcgccgctg
     1141 gtgtatcagg tggcgccagg gttccgtgcg acgaagacag gccccgaggg cttcccggtc
     1201 gtcgtgcgaa tcaatcagag cagcctgagg atcacgaacg ggtccgaagt gcgatggacg
     1261 tgcgcggtgt ggctgccacc ggcgcccgtc ttctcggcag gcgtgcgcct tgcgccgacc
     1321 gagtcccgtg atcttcccta cgaaagcttt accggccaat ccggtaccct caccgacgga
     1381 accggctaca ccgggcgcgg agggcgattg aagtccactg cctcgaggac tcggggcgcc
     1441 ggcatcgcgc ccgattcttt tgagcggtcc cagcggtcat tgttgactcc tgactgttga
     1501 atcgtggaaa tcgacaatca acgatcccca atcaacaccg aatcaagaac cagagatcgc
     1561 taatctgcaa tcgtcctgca tgacgctcgc gcccagaatc gaaggcactc tcccgatccg
     1621 ctcgcctgcg gcggcgtttc tgcaggcgtt cagccggagg gccgaaagcg gtctgctcac
     1681 ggggcgcccg cagcctcgat cgagctacgc cgcgactgtc gtgtccgacg gcgggctacg
     1741 aattcgcgcc gccgactggt ccaccgccat tagcgtcggc ctcaacgatc tggaactgcg
     1801 tccggcgcac gacggcgcgg ttcggtatcg gctgcagtac tggcgatgga cctgctacgc
     1861 cgtcgggctg agcggcgtcc tggggggcat cggtctctgg ctgctgctgg ccttcgatgc
     1921 ccgcgaatac atcgcgaacc atcccgactc ccagcttccg ggactgtcga tcgatcagaa
     1981 cctgctcatg gcgtgggcga tggttctctt ctggggattc gcctggccct ggatcctcgt
     2041 cgcgttgcac aagcgcccgc ttcgtcggct gatcgcgcgc attgcggccg aggtggatgc
     2101 aagcgtcgcc tgaaccttca ccgcgaacac gtctgaggct ctcacaaacc acgaggggca
     2161 cgaagaattt gccacgaagg gcacgaagat cgcgatgatt cgtggctacg cgggctgacg
     2221 gctcgcgctc atcgccgccg tgaccgccgt cgtcatctcc ctcgtggtgg ctcggccgcc
     2281 gaggtccggc gtcttcacgc gctcgtcgga gagtacccgc tcgatagcgg cgagaatccg
     2341 gtcgtgcgac gcgcgctggc cgaggtgatc gagcatcatc gctcccgccc agatggcggc
     2401 gatgggattg gcgatcccct tccccgcgat gtcgggcgcc gacccgtgaa tcggctcgaa
     2461 catggacggc gtcgtcttgt cggggttgat gttgccgccg ggcgccacgc ccaggctgcc
     2521 ggagatcgcc gaaccgatgt cggtcaggat gtcgccgaac aggttcgacg ccacgatcac
     2581 gtcgagcgtg tgcgggtggg tcaccatccg cgcgcagagc gcgtccacgt gatacttctt
     2641 cagttcgacg tcgggaaact ccttggcgac gccggcgacg acctcgtccc acatcaccat
     2701 cgcgtgctgc agcgcgttcg acttggtggc gctggccagc atcttccgcg gcctcgaggc
     2761 cgccaggcgg aacgcgtagc gggcgatgcg ctcgatgccg tgacgcgtga agacgcccgt
     2821 ctgctccgcc acttcctgcg gcgttccacg gtgcagccgt ccgccgatgc cgcagtactc
     2881 accttccgag ttctcgcgaa cgcagatcat gtcgatgtcc gattggcgcc gtccggccag
     2941 cggcgacgag atgccgggca ggagccgcat cgggcgcagg ttgacccact gaccgagctg
     3001 ctgccgcagc ggcagaatca gttcccgcgc cgagatgtgg tcggccaccc gcggatcgcc
     3061 gatggcgccc atgtagatcg cgtcgaactt caagagctga tcgatgccgt cggcatccat
     3121 catgcggccg tgctgaaggt agtagtcgca tccccagggg agcgtggtga actcgcagcg
     3181 gaatccccct tgctcggcgg ccagcttcag gacctcgatg ccggcgggag tcacttcttt
     3241 tccgatgccg tcgccggcaa ggactgcgat tttgtaagtg gtcatggtca gtctacgggc
     3301 taccgtctcc ggtccctgcg agcgatcaac atcacggcaa aggcgattcc cgcggcagcc
     3361 agtccggcga gcgtcggatc cagccacggg tagcgtccgg caaacccgaa gcgtaccacg
     3421 aggagcgtca ccacgccggc ggcgatggcg ccgagcgcct cgggctgtcc cgccctgcgc
     3481 gaccacaacc cgcccagcac cggcacgaac aggcttacgc ccagcagcga gtagaacacc
     3541 gtcatcgcct ggacgatcgt gctcaggtag atcgacagga gcacgccgag cgcgccgccg
     3601 ctcaccgcgg acaggcgcgc cacgcgaagc aactccgcat cggtggccga tggcttcacg
     3661 taccgctggt acaggtcctt cgacgccgac gtcgagatca tgaagaggat tgcatcgcag
     3721 gtgtccacct cggtcgagaa caccgccgcc agcgcgagcg cgccgagcca cgcgggcagc
     3781 agctgggtga gcaccatcgg aagcacgagg tcagggctgc tcacttctgg aagggccgcg
     3841 cgcccagcca tgcccagcat gacgggaagg aatgcgaaca gcatcagcgc gacgccgttc
     3901 agcgccacgc cgattcgcag cgcccgagcg ctcgccgcgc cgtacgactt ctggatgagg
     3961 cctggcgaaa tcacgaacgc gggcccggtc aatgccagga gtgtccatcc cgatccaggt
     4021 cctgatgaat acgtgatgtc ggcgtaggtc gccggcatgc cgtaggctaa cgcgtcaacc
     4081 ccccctgcgc ccgcgatcac gaacgccgcc gcgaggatga acccgacgag catgacgacg
     4141 agctgcaggc tgttcaccca ggcagttccg agcagaccgc cggccgtgaa gtagatcgtc
     4201 atgatggcgc cgccgacgag ggatccggcc cagcgcggcg cgccggtcac gacgttgagg
     4261 atcgctgcgc ccgcgatgag ctgcgcggcg aggatccaca gcgagccgaa cgcgacgagc
     4321 ccggtaacga tgccgcgaac cgccggtccg tacctgtgtt cgaggaagtc gcccgtcgtg
     4381 tagaagccgt actgctttgc cagcgtccac agccgcgggc ccacccagaa ggcgagcacg
     4441 agcgacgcca ggcccgccga tccgctccac caccacgcgc tcagcccctc gcggtacgca
     4501 agtcccgcga cgttgacggt cgcgccggcg ccgatgtttg ccgcgagcat cgacgcgaac
     4561 acgaggccgg gcccgagact ccggcccgcg acgaagaacg cgctgctggt ccggacgaag
     4621 cgggccgtcc agatgccgac gccgacgacg gtcagtgaat agacgacgat gaggacgaga
     4681 tgagggctca ccgaaagcgc tgcggaacgg tgcccagatc gcgagcggcg ggcaggccgc
     4741 ccgacgaagt ggcttgcacg gccgcgcgca cgatggccgc cgacagcgcg tcggctgcga
     4801 gagcgccgat cgccgtcaca tcgctccgcc cctgccacga tccggttgcc agcgcgaaca
     4861 ccgtgtcgcc gtcggccggc gtgtgcgccg gatagatcgc tcgtgcgagc ccgtcatcgg
     4921 ccatcagcgc cacgcggccg gcctcggctt tcgtgagacg cgcgttggtg gcgacgacgg
     4981 cgatcgtcgt gttctcgacg ggccgcggtc cgcccgttgc ggccggggat cgcagcagca
     5041 cgcgcgcgtc cgccaggcgc ttgccgtcgg ccgtgcgcac gcccgcgacg acggcgcccg
     5101 tggctgggtc cacgacatct ccaacggcgt tgaccgcgac gatcgccccg acgacgaggc
     5161 cgcctggaag agcgatggac gcagaaccta ctcccgcttt catcgcgcgc gcgcggctgc
     5221 cggcgatctt accgacagtc gcgccggctc ccgcgccgac gttgccctcg gcgacggggc
     5281 cgttgctcgc ggccatggcg gcgcggtatc cgcagtcggc ggtcggcctg atgctggagc
     5341 tgccgccgaa cccgagatcc atcagcaccg ccgc
//
LOCUS       DBNGSH010000665         3452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3805197, whole genome shotgun sequence.
ACCESSION   DBNGSH010000665 DBNGSH010000000
VERSION     DBNGSH010000665.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3805197"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="AC16CY_15315"
     CDS             complement(<1..733)
                     /locus_tag="AC16CY_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3355217.1"
                     /translation="MRRLLFALVIAITLAAPLPAASVDGIRIHSSTTGKGKATLVFVH
                     GWTCDSSSWAAQVPAFAGKYRVVTLDLPGHGQSGSPKDGKFSMDLFARAVEAVRDDAR
                     AERVVLVGHSMGAPVIRQYARLYPKRVAGLVAVDGPLDLRKFGLPAGQKPPVMTGPEG
                     LKFRENMIRSMFTPQTPPALQQQILGMMLKAPEATAAGAFEAVFDPAIRTAEVTSAPA
                     LAVYAGTAQVPEVGAVKELFPNYEAT"
     gene            complement(752..2512)
                     /gene="mutL"
                     /locus_tag="AC16CY_15320"
     CDS             complement(752..2512)
                     /gene="mutL"
                     /locus_tag="AC16CY_15320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR3355218.1"
                     /translation="MGKIRQLPADLANQIAAGEVVERPASVVKELVENSIDAGARRIV
                     VTAEFGGKRLVRVDDDGEGMGPEDARLAIERHATSKIRKADDLGRIATLGFRGEALPS
                     IASVSHFALRSRARGENSGTEVRVNGGTIESVVEAGLPEGTSIEVADLFYNLPARRKF
                     LKSDAAEAAQVSRVVTQLALCYPEVGFTLTNSGRKVLQCPPVAGLRDRLYQLYGERSD
                     LVDLQRVSGDVKIVGFVAALAEQGPTRGPQHVFINRRIVKDRTIAHAIIDAYSVASIK
                     ERSPEVHLFIEMPPDAVDVNVHPTKAEVRFRDQSFVHEIVRRALGDALGRGPAPELQL
                     ASAQAGQAQATTLPLPHAYSAVVPSRWVAGAPMSEAGFAPTGVSGIAAAAPTGEAGFA
                     PSIKPLMPLGQFRDTFIIAVDEDGIAIIDQHVAHERVLFERITERLTRGRLESQRLLQ
                     PMVVEMSSAARQALVSRPADLERLGFELEEFGGDALRVSAFPALLTRQQCEAALRSLA
                     EDLEGLDRASTVEDAIKRIAATTACHAAVKANDPLTMEKMAHILEELRATAYSTICPH
                     GRPVMLRLTRREVEKNFQRI"
     gene            complement(2642..>3452)
                     /locus_tag="AC16CY_15325"
     CDS             complement(2642..>3452)
                     /locus_tag="AC16CY_15325"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR3355219.1"
                     /translation="FHPLSIDDARSALQFPKVEPYPEYLYLVLHGIDAEESTRRFSTR
                     DVDFFLGRNFLVTVHDGRSRSISRLREVCNQHARVLSEGPVALLHRIIDTMVDNYRPV
                     AEALEDRIEKLEEHSMAGRTQMVQQVLKVKRELATMRRVLIPQRDAVGRLARREFPAI
                     SDEMAFRFRDVYDHLVRLSEEAMLFQDRMTGVLEVNLASVSNRLNQIMKVLTVMSTIF
                     LPLTVLTGMWGMNIQLPHFPGGDGVQFLWVGGIMAGISLAMLGLFRFNKWI"
BASE COUNT          590 a         1201 c         1083 g          578 t
ORIGIN      
        1 gcgtggcctc gtagttcggg aacaattcct tcaccgcccc gacctctggc acctgcgccg
       61 tgcccgcgta gacggccagc gcaggcgccg acgtcacctc ggcggtccgg atggccgggt
      121 cgaacacggc ctcgaacgcg ccagccgccg tggcttccgg cgctttcagc atcatcccca
      181 gaatctgctg ctggagcgcg ggaggcgtct gtggcgtgaa catcgaccgg atcatgttct
      241 cgcgaaactt caatccctcc ggccctgtca tgacgggcgg cttctgcccg gccggcagcc
      301 cgaacttccg cagatcgagg gggccgtcca cggcaaccag cccggccacc cgcttgggat
      361 acagcctcgc gtactgccgg atcaccggcg cccccatgct gtgaccgacg aggaccaccc
      421 gctccgccct ggcgtcgtcg cgcaccgcct cgaccgcgcg ggcgaagagg tccatcgaga
      481 acttcccgtc cttcggggat ccgctctgtc cgtgtcccgg gaggtcgagg gtgacgacac
      541 ggtacttgcc cgcgaacgca ggcacctgcg ccgcccacga cgacgagtcg caggtccagc
      601 cgtggacgaa cacgagcgtc gccttgccct tgcccgtcgt tgacgaatga atccggatgc
      661 cgtcaaccga cgcggccggc agcggcgccg cgagcgttat cgcaatgacg agagcgaaga
      721 gcagccgtcg catgaagcct ccttgtggca cctagattct ttgaaagttc ttttcgacct
      781 cgcgccgcgt caagcgaagc atgacgggcc gcccgtgggg gcagatcgtc gaataggccg
      841 tcgcgcgaag ctcctccagg atgtgcgcca tcttctccat cgtcagcggg tcgttggcct
      901 tcaccgcggc gtggcaggcc gtcgtggccg cgatgcgctt gatcgcatcc tccaccgttg
      961 acgcccgatc gagcccctcg agatcctcgg ccagcgacct gagcgcggcc tcgcactgct
     1021 ggcgcgtcag cagcgccgga aacgccgaca cgcgcaacgc gtcgccgcca aactcctcga
     1081 gctcgaagcc aaggcgctcg aggtcggccg ggcgcgacac caacgcctgt cgcgccgccg
     1141 acgacatctc gaccaccatc ggctgcagca ggcgctggct ctccaaccta ccacgtgtga
     1201 gccgctcggt gatccgctcg aacagcacgc gctcgtgcgc cacatgctga tcgatgatgg
     1261 caatgccatc ctcgtccacg gcgatgatga aggtgtccct gaactggccg aggggcatga
     1321 ggggtttgat actcggtgcg aaccctgctt cgcctgtcgg tgcggcagct gcgattccgc
     1381 tgacgcctgt gggtgcgaac cccgcttcgc tcatcggtgc gccagcaacc caacggctgg
     1441 gaacgacggc cgagtaggca tgaggcagcg gcagcgtcgt cgcctgcgcc tgccccgcct
     1501 gcgccgatgc gagctgcagc tcgggcgcgg gcccgcgtcc cagcgcgtcg ccgagggcgc
     1561 gccgcacgat ctcgtggacg aacgactgat cccggaaccg gacctcggcc ttcgtcggat
     1621 gcacgttcac gtccacggcg tccggcggca tctcgatgaa caggtgcact tccggactgc
     1681 gctccttgat cgaggcaacg ctgtaggcgt cgatgatggc gtgcgcgatc gtccggtcct
     1741 tgacgattcg ccggttgatg aacacatgct gcggcccgcg agtcggcccc tgctcggcca
     1801 gcgctgcaac gaagccgacg atcttgacgt caccgctgac ccgctggagg tccacgagat
     1861 cgctccgctc gccgtacaac tggtacagcc gatcgcgcag cccggccacg ggcggacact
     1921 ggagcacttt tctgccggag ttggtcagcg tgaaccccac ttccgggtag cacagggcga
     1981 gctgcgtgac gacgcgcgac acttgcgcgg cctcggccgc gtccgacttc aggaatttgc
     2041 ggcgcgctgg caggttgtag aagagatcgg cgacctcgat cgacgtccct tccggcagtc
     2101 cggcttcgac gaccgactca atcgtgccgc cgttgacgcg aacctcggta ccggaattct
     2161 cgccccgggc ccgcgatcgg agggcgaagt gcgagaccga cgcgatgctg ggcagcgcct
     2221 cgccccggaa gccgagcgtg gcgatccggc ccaggtcatc ggccttgcgg atcttgctgg
     2281 tcgcgtggcg ttcgatcgcc agccgcgcgt cctccggtcc catcccctcg ccgtcgtcat
     2341 cgacgcgaac cagcctcttg ccgccgaact cggcggtgac gacgatgcgc cgcgccccgg
     2401 cgtcgatgga gttctcgacc agttccttga cgacggacgc gggccgctcg acgacctctc
     2461 cagcagcgat ctggttcgca aggtccgcgg gcagctggcg aatcttgccc atacgtcatt
     2521 caaccacgaa ggccactaaa cgcgtcacgc acgccaacca cagagtcaca gaggacacag
     2581 agacgtgaat gcttgctctg tgttctctga gcctctgtgg ttaacgtccg tgtcagtccg
     2641 atcagatcca cttgttgaaa cgaaagagcc ccagcatggc caggctgatt cccgccatga
     2701 tccccccgac ccagagaaac tgcacgccgt ccccgcccgg gaagtgcgga agctggatgt
     2761 tcatgcccca catcccggtc agcaccgtca gcggcaggaa gatcgtcgac atgacggtca
     2821 gcaccttcat gatctggttc aacctgttcg agacgctggc caggttgacc tccagcaccc
     2881 ccgtcatgcg gtcctggaac agcatcgctt cttcagacaa gcggacgagg tggtcgtaca
     2941 cgtcgcgaaa acgaaacgcc atctcgtcgg agatcgccgg aaactcccgc cgggcaagcc
     3001 ggccgaccgc gtcgcgttgt ggaatcagca cccggcgcat ggtcgccagc tcgcgcttga
     3061 ccttcaacac ctgttgaacc atctgcgtcc ggccggccat cgagtgctct tcgagcttct
     3121 cgatgcgatc ttcgagcgcc tcggccaccg ggcgatagtt gtccaccatc gtgtcgatga
     3181 tgcggtgcag cagcgcgacg gggccctccg aaaggacgcg cgcgtgctgg ttgcacacct
     3241 cgcgcagccg ggagatgctg cgcgaacggc cgtcgtgtac ggtgacgaga aaattccgcc
     3301 cgagaaagaa gtcgacgtct cgcgtagaga agcgccgggt gctctcctcg gcgtcgattc
     3361 cgtgcagcac gaggtacagg tactcaggat atggctcgac tttcggaaac tgcagcgcgc
     3421 tccgcgcgtc gtcaatcgag agagggtgga ac
//
LOCUS       DBNGSH010000666        18896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_919570, whole genome shotgun sequence.
ACCESSION   DBNGSH010000666 DBNGSH010000000
VERSION     DBNGSH010000666.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 18896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18896
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_919570"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="AC16CY_15330"
     CDS             <1..517
                     /locus_tag="AC16CY_15330"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR3355220.1"
                     /translation="RQNAVDLIAGSNKDEHTSMGGNVAFRDTLAWGMRLFAERQAAIG
                     KRAYWYLFTHEPPVDPGGRDLKATHAAEIVYAFDNLSAPRVYPDASSPRLAMASARDR
                     AMAAQMSSYWVNFAKTGDPNGRGLPEWPRFKDRNVPPHILGEIREFPGAQTLNAYDEQ
                     YAKILAALLKR"
     gene            complement(506..1732)
                     /locus_tag="AC16CY_15335"
     CDS             complement(506..1732)
                     /locus_tag="AC16CY_15335"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3355221.1"
                     /translation="MKLSRRDVLRTAGTVVAPFLALNGARAAVPRLRVRGVDLLPVRA
                     TNRTVWLIVRLQTDAGLVGFGEASDAFGFANTTSDDTARMRSQLKAFFDLVNGRSPFE
                     IERYRQQGEPLARQGLVAATAFSAIEQALWDLAGKALDVPAYVLLGGAVRTELPVYAN
                     INRATNPRTPAGFASAARRAVSDGFGAIKAAPFDGFPAPGSSAAEIARAIDTGIDSVG
                     AMRDAVGPKVAIMIDCHSFFDVEQAVRVAGRLEPQNLAWYEEPVAPERVDETLAIKSR
                     IKQPMAGGETLFGLSGFGPLIRRRAFDTIMPDVKHCGGLLELTRIAAMAADAGVMVAP
                     HNPSGPVSTAASVQVCAGMKNVNYLELQYGEVDWRSGVLTPPERFINGRIQVPDRPGF
                     GIELNEAVIRSRSLPL"
     gene            complement(1742..2689)
                     /locus_tag="AC16CY_15340"
     CDS             complement(1742..2689)
                     /locus_tag="AC16CY_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355222.1"
                     /translation="MRRRAFVAAGVAASLGGAAWAALSSSWSARFLRQRADEIGRDVP
                     AAPHTPSPSSWSDNAVTLAWLGHATVLVNFYGVRILTDPTLYPRIGVDLGVGTLGPLR
                     LVQCALTPDDVPDVDLVLVSHAHFDHLDVPSLGAIRGQPAAVMGPGTSDLLPRRPYSS
                     VRELRWNESVTVKTTRGEVLVRAIEVKHWGARIRRDTWRGYTGFVVEREGRRILIGGD
                     TAATPLFGNHRRFGSFDAAVMPIGAYNPWIANHCTPEQAVVMADAAGARLIVPVHHQS
                     FRLSNEPFMEPIERIQDALAKEEDRLAIREIGQTIVLQA"
     gene            2831..3688
                     /locus_tag="AC16CY_15345"
     CDS             2831..3688
                     /locus_tag="AC16CY_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR3355223.1"
                     /translation="MNLRLPALVLSFALALTTLAAQRDADRRDWLQLFNGKDLDGWTP
                     KINGYPLGENYGNTFRVENGVLAVSYDQYGQFDSKFGHLFYSRAAFSHYILAAEYRFV
                     RDQAPGGPSWAVRNNGLMLHSQPPQSMGLNQDFPISIEVQLLGGAPAPDRTTANVCTP
                     GTEIFMKGAMVRGHCTNSTSAIYRGDAWVRVEVEVHGGERVTHRLDGQTVLEYEMLQI
                     GGGSVNNFDPAVKKDGAPLTRGYIAIQGESHPTEFRKIELLNLSGCMNPQSPSYRSYF
                     IDRDDSQCR"
     gene            3768..4328
                     /locus_tag="AC16CY_15350"
     CDS             3768..4328
                     /locus_tag="AC16CY_15350"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3355224.1"
                     /translation="MAPRLRSGREPAIDPGSLRPAAALLLMYPIELAWHIPLTVRALD
                     LRHHTGQVSLPGGRIDPGETVEGAALREADEEVGLERRDVVVLGRLTPLPIFVSGHLL
                     HPVVGIAADRPRFAVASGEVDRLLELPVTRLSQPDAVAWEERERSRPPYGVMDVPYFD
                     VHGARVWGATAMILAEFLDLLGDLDI"
     gene            4440..4955
                     /locus_tag="AC16CY_15355"
     CDS             4440..4955
                     /locus_tag="AC16CY_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355225.1"
                     /translation="MGPLRELIAVDERRALEYFVAGLQEVCGTDVDRHELLYNASVLA
                     HYAQVSTHADLDMPAPTALGEVFDHFVIESSVPCDGLIMETAGAQCLLLTGFFENQMR
                     GRYNLRWYAELGAGFFIRAAAQQRSTHKARLLQMVGRHFEPWRQRHARLGRELRDQVY
                     LLPPTPPAPLM"
     gene            complement(4975..5340)
                     /locus_tag="AC16CY_15360"
     CDS             complement(4975..5340)
                     /locus_tag="AC16CY_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355226.1"
                     /translation="MNRARSTLLILALAALTTGCASRSINQLLSDPSRYRNKEVTLSG
                     SVVNSYSVLGSGAYQLDDTTGRLWVVSNAGVPRQGARVTVKGTVREGFNLGSLGDRLK
                     LPSGVSAGVVLMESSHKAD"
     gene            complement(5420..5848)
                     /locus_tag="AC16CY_15365"
     CDS             complement(5420..5848)
                     /locus_tag="AC16CY_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355227.1"
                     /translation="MKSLLSLLIAGAVLSGTVLTGTAEAGDRRKGSKHRRVEVRYEDR
                     YDDHYRYGRSDRYFRDRDVVVIRNYYQPYYHPLPPGLQRRYYRTGYLPPGWAKRMRPV
                     PVYVERELVAVPYGYHRGIIDGHAVIYNDRGFVLDVAVLF"
     gene            complement(6074..8209)
                     /locus_tag="AC16CY_15370"
     CDS             complement(6074..8209)
                     /locus_tag="AC16CY_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3355228.1"
                     /translation="MRTITAGGIIVVAILAVSQPDPLAQQSNPPARSAPTFTAAQAAL
                     GEAAYAASCAQCHGSSLSDGAFGPPLKGVPFIQKYGGRSVEALFTVTSNTMPTTSPAS
                     LSAGVYAQIVAYILQQNAILPGSVELPADPGRLASMIIPQGGFSIMAYSPYAASASKV
                     VRPTPLDKYTPVTDAMLSDPAPSEWLAWRRTWDAHGFSPLTQINKSNVGNLRVAWSWS
                     LPPGSNEGIPLVHDGTLFVHGMGDRVQALDAKSGDLLWEYRRRLPQGVSPTVKRGMAL
                     YRDRLFIGTSDAHVIALDAKTGRLVWDTPVGDTKVREGIAGGVLAARGKVMVGTTGTG
                     VGAKPGGPQIVGLDAGTGAVAWRVNTIAQPGEPGGDSWNGMPISQRSGASVWTTGSYD
                     PETGLAYFGTGNTYDTGPLMPPSSRPGVTNDALYTDSTLAIDPDTGKLAWHFQHHPND
                     QWDLDWAFEQQLVRLPVRGQTRTLVVTAGKLGIFDAVESATGRFAFSIDVGLQNIVTE
                     IDPETGVKTIDASLIPGDGKLKMVCPHGAGAKNYLPASYNSTSRTVFVPLTEACMDVF
                     PIPGGAGRGALSSGVNWGIRPRPDSDGNFGRLQAINLETRQTVWTTRHRAPQTSGVLA
                     TAGGVVFAGAFDRVIRAYDDATGKVLWQTRLNDVSSSSPISFSVDGKQYVAVVVGQGG
                     FHAASYAVLVPELTSPPDRGAAIWVFALP"
     gene            8280..9065
                     /locus_tag="AC16CY_15375"
     CDS             8280..9065
                     /locus_tag="AC16CY_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355229.1"
                     /translation="MIHVTNLVKTFGEVKAVDDISFDVAAGEIFAFLGPNGAGKTTTI
                     QMLTTLQRPTGGSMQLDGLDPVTQPLQARSRFGIVFQDPSLDSELTAYENMDLHGALY
                     HVPRKIRRERTETLMKLFELWDRRDSMVKTYSGGMKRRLEIARGLLHTPRILFLDEPT
                     LGLDPQSRNQLWTHVKHLNETEGVTVFLTTHYMDEAERTAHRIAIIDHGRIVAQGTAE
                     SLKQQTGAESLEGAFLALTGNTIRDERADSADQLRQIARMWRR"
     gene            9069..9815
                     /locus_tag="AC16CY_15380"
     CDS             9069..9815
                     /locus_tag="AC16CY_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355230.1"
                     /translation="MTVVYYLWLREVKRYLRSRPQIIGSIAQPLLYLLVLGFGLGPVF
                     AQSGQGSYLQFIAPGVVGMAILFTSIFSGIGLLWDRQFGFLKETLVAPVPRLQIMLGR
                     TLGGATIASIQGALILVVCIIAGFRPQSLAALPLALAFMLLVGLVFAGLGTAVGSTLK
                     DMQGFQLIMNFLVMPIFFLSGALFPLNNLPAALTIATRADPLSYGIDGLRGALIGVTQ
                     FGVATNLSVLAVVASGFLVLGAWAFSRIQV"
     gene            9966..10346
                     /locus_tag="AC16CY_15385"
     CDS             9966..10346
                     /locus_tag="AC16CY_15385"
                     /inference="COORDINATES: protein motif:HMM:NF039183.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HIR3355231.1"
                     /translation="MASVPAVVSTSGAPTSFPREGVVRRARRLSSAEVLWLAFVSVQA
                     LDGAMSYIGVTLHGPAIEANPVVAWYMGAFGPAVGFTAAKLFAVMCGVVLYVTARHKW
                     VAVLTLLYLVFAVGPWARILAISH"
     gene            10407..10874
                     /locus_tag="AC16CY_15390"
     CDS             10407..10874
                     /locus_tag="AC16CY_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355232.1"
                     /translation="MYTALLILHSWLRWAVIIAGLAVLAGSVTGLTRRRPWQKSDDLR
                     IRVFTTSLDVQFLIGILLYGVLSPVTYAGFADMGAAMRDPILRFYTVEHIFGMVAALA
                     LAHIGRARVRKAAEAARHRTVLIFFGMAIAVILLSIPWPGTPGGRVLFRGFGS"
     gene            10903..11178
                     /locus_tag="AC16CY_15395"
     CDS             10903..11178
                     /locus_tag="AC16CY_15395"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3355233.1"
                     /translation="MRVARRIVVGGRVQGVGFRYFTQDVALREGVTGWVRNLPDGRVE
                     VHVEGETEAVTRVEAAIRRGPRGARVQTVDVVGEDPSGLYDTFDVTS"
     gene            11191..11703
                     /locus_tag="AC16CY_15400"
     CDS             11191..11703
                     /locus_tag="AC16CY_15400"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR3355234.1"
                     /translation="MDLKQHIRHVPDFPKAGILFYDITTLLRDPKGFEATIDRLSAPY
                     EEDSIDLVVGIESRGFILGSGVAQRLGAGFIPIRKPGKLPSHRIKETYELEYGTDALE
                     IHADAVQSGQRVLIVDDVLATGGTASAAARLVRQLGGDLHGLAFLIELLFLKGQSKLP
                     GEKVFSVLQY"
     gene            11754..11830
                     /locus_tag="AC16CY_15405"
     tRNA            11754..11830
                     /locus_tag="AC16CY_15405"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11788..11790,aa:Arg,seq:cct)
     gene            11875..12627
                     /locus_tag="AC16CY_15410"
     CDS             11875..12627
                     /locus_tag="AC16CY_15410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3355235.1"
                     /translation="MKRKERHHLKENELVHTIGAARDFFDARQREIGVVLVGIIVVAV
                     VAAGVMLFRQRNQSRGSDLLAEAMVALNAQVVPAGAKAGEDNLPAAATIGATGSFSTE
                     AAKLTAALPKLQAAADAYPDSAAGITARYHLAGAFAALGRHEDAIKQFDEVATRAGVG
                     SVYGRMARLGKADAQARAGQLDAAIATWKEMAGVSSPDYPTDAILMELARAYAQKGDK
                     EEARKAFTQIVDQHPDSPYAPQARAELENLKG"
     gene            complement(12639..14174)
                     /locus_tag="AC16CY_15415"
     CDS             complement(12639..14174)
                     /locus_tag="AC16CY_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3355236.1"
                     /translation="MTPRRAALVALVAGAAWGCRAAAPADPRIITIAVRSGPTTLDPR
                     LGNDEATQRMSQLIYSPLLEHGDDLRVRPALASRLDNPDPLTYIAHLRRGVRFHDGHE
                     LTSRDVVYTFRSVLDPGLVSPFKGAFRLLKEVTALDDYTVRFRLSEPFAAFPLQLVGV
                     PPIVPADAGDSLKVRPIGAGPYRFVRRDVDDTLTLSAFDDYWGGAPDNAGIVMKVVPD
                     DVMRGLELRKGTTDLVVNDLPPDIVYQLDKSGAFTIQRSAGLDLFYLGFNMRDGVLRD
                     VRVRHAIGYAANRDAIIQYLRRGLGRPASGLIPDLAWAFEPDVHRFTYDPERAGRLLE
                     DAGYRDPDGPGPRPRLRLSLKISNSEETRIQAAAIQQDLRSVGIDLDIRSYEFATFYA
                     DVLKGNFQIFALQWVGGAMVDPDMLRRVFHSSELPPSGFNRGLYQNEEVDRFLELATT
                     SLDEADRKRYYSRAQQIIALEAPYIPIWNRTNVVVAQRSLDGLHIGPLGDFTALREVR
                     RRP"
     gene            complement(14174..16843)
                     /gene="mutS"
                     /locus_tag="AC16CY_15420"
     CDS             complement(14174..16843)
                     /gene="mutS"
                     /locus_tag="AC16CY_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR3355237.1"
                     /translation="MSSTITATPAMRQYLDAKRQHRDAIVFFRMGDFYEMFYEDALVA
                     ARALELTLTSRSKDAGGNGIPMCGVPFHAADGYIARLVRKGFRVAICEQVEDPKKAKG
                     LVRREVVRVVSPGTLTDSSYLEAREPAFLMAIASSGPAGAETFGAALIDLSTGEFSAA
                     EYVGPEAFSALRDELAVLRPREIVVPGGQAPQDPSSVAARLGEMSHLQFAVTAVDDWS
                     FEPEAARRALLDQLEAHGLEGFGLEGRPAATRAAGGLVTYLRETQKAGLAHVRAITYR
                     TSSDSLVIDPITLKHLEVVAGSEGGVQGSLLHEIDRTATAMGGRLLRSWLLRPLRALE
                     PIQDRLDAVEELAFRASERGKFRDALKGVHDLERLIGRIALGTAGPRELVSLRQSLAV
                     VPRVTLLLEPLQAPLLRSLVAGLDDLPDLRGRIERTIIDEPPAIAREGGFTRDGVDPE
                     LDELKAISRSGRQVIAEMEERERARTGIASLKVRFNRVFGYYIEISKANLHLAPSDYQ
                     RKQTIAGGERFTTPMLKEYEEKVLGADGRILVRELEIFEDLRRVVAAESTVIQDTARA
                     LATLDVLAGLAETATIANYTKPHLHDGDELVVTDGRHPVVERFTDAFVPNDIDLNGTT
                     RQLVILTGPNMGGKSTYLRQTAIIPLLAQIGSFVPARHAKVPIVDRIFARVGASDNIA
                     RGQSTFMVEMQETSNILHAATSRSLVVLDEIGRGTATFDGLSIAWAVAEYLASNPKAR
                     PKTLFATHYHELTDLAGTLPGVANAHVTAREWKDQIVFLRKIVSGRSDRSYGIQVARL
                     AGLPAGVVARAQQILAGLENDEASRGGRPTLSGAAGDPSTAQLGLFAARPEAAPGPDA
                     GIRARLDAVNVEDTTPRQALEILAELKKLSEDT"
     gene            16873..17559
                     /gene="tsaB"
                     /locus_tag="AC16CY_15425"
     CDS             16873..17559
                     /gene="tsaB"
                     /locus_tag="AC16CY_15425"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3355238.1"
                     /translation="MLVLALDTTTPSGSCALAADGVVLREEGSNASRSAAARLPADLM
                     TILERAQRRLDEVEVFAVATGPGSFTGLRIGIATMQGLAFAAGKPLVGVSGLEALARI
                     GLEEAAGRRIAAWVDAWRGEVYAALYEQGSEVEPVVARPDVLLSQYAGEPTLFIGDGA
                     RTYREQIHARGGPAWRVADPAAPMLAGTIATLAASLVAAGTAPPPHLIRPLYVRRTDA
                     ELARHGRATG"
     gene            17546..18028
                     /gene="rimI"
                     /locus_tag="AC16CY_15430"
     CDS             17546..18028
                     /gene="rimI"
                     /locus_tag="AC16CY_15430"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR3355239.1"
                     /translation="MPPDSPPEYWIDRLQSDADLDGVLEVEAETFTNPWTREMYAWEL
                     QNRSICHIFVVRTPDRPVVGFCAFWLVVDEIHVNNVAVLPGLRGRGMGTALMHRVFEE
                     AARLGARRATLEVRESNEGARRWYEFLGFRVAGTRRHYYTNPVEDALILWREDSAGPA
                     "
     gene            18111..18353
                     /locus_tag="AC16CY_15435"
     CDS             18111..18353
                     /locus_tag="AC16CY_15435"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1242/YdcH family protein"
                     /protein_id="HIR3355240.1"
                     /translation="MSAAEAQEVKNLLLRSNEQYRQLSERHHQLDDRLHELTARPYLT
                     DSQRFEEVTLKKRKLALKDQMEQMALEYAKTHIRAS"
     gene            18391..>18896
                     /locus_tag="AC16CY_15440"
     CDS             18391..>18896
                     /locus_tag="AC16CY_15440"
                     /inference="COORDINATES: protein motif:HMM:NF014696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR3355241.1"
                     /translation="MRIDPAGWPFVLGGSALAVLAWLLGGPVYGATLLALPAFFLFFF
                     RDPERTHHAPALGVVSPADGRVMVAGAPTGEAVPPGRWQQISIFLSPLDVHVNRIPVS
                     GRVTRVEYHPGRFLPAYRRESGDLNEHTEVVIEQRGQQVVVRQIVGVLARRIVCRTTE
                     GAEVRAGDR"
BASE COUNT         3055 a         6159 c         6455 g         3227 t
ORIGIN      
        1 caggcagaac gcggtggatc tcatcgcggg atccaacaag gacgaacaca cgtccatggg
       61 cggcaacgtg gccttccgcg acacgttggc ctgggggatg cgtttgttcg cggagcgcca
      121 ggcggcgatc gggaagcggg cttactggta cctcttcacg cacgagccgc cggtcgaccc
      181 agggggccgc gacctgaagg cgacccacgc cgcggaaatc gtctatgcgt tcgacaatct
      241 ctcggcgccg cgggtgtacc ccgacgccag ctcgcccagg ctggcgatgg cgtcggccag
      301 ggatcgcgcg atggcggctc agatgtcgtc ctactgggtg aacttcgcga agacgggcga
      361 cccgaacggg cgcggcctgc ccgagtggcc acgcttcaaa gaccgcaacg tgccgccgca
      421 catcctcggt gagatcagag agtttcctgg cgcgcagacg ctcaacgcct acgacgagca
      481 gtacgcgaag atcctggcgg cgctgctaaa gcggtagcga tcgcgagcgg attacggcct
      541 cgttcagctc gataccgaag cccgggcggt cgggaacctg aatgcgtccg ttgatgaacc
      601 gttccggcgg cgtcaggaca cccgagcgcc aatccacttc tccgtactgc agttcgagat
      661 agttgacgtt cttcattccg gcgcacacct gaacgctcgc cgccgtagag acgggtccgc
      721 tcggattgtg aggagcgacc atgacgcccg cgtcggcagc catcgcggca atacgagtca
      781 gctcgagcag cccgccgcag tgcttgacgt ccggcatgat ggtgtcgaag gcacggcggc
      841 gaatgagcgg accaaatccg gagaggccga acagcgtctc accgcctgcc atcggctgct
      901 tgatccggct tttgatcgcg agcgtctcgt caacgcgctc gggggcaacc ggttcctcgt
      961 accacgcgag gttctgcggc tcgaggcgcc cggccacgcg cacagcctgc tcgacgtcga
     1021 aaaagctgtg gcagtcgatc atgatggcga ctttcggccc cacggcgtct ctcatcgccc
     1081 ccaccgagtc gatgccggtg tcgatggcgc gggcgatctc ggccgcagac gagccgggag
     1141 cggggaagcc atcgaagggg gccgccttga tcgcgccgaa accgtccgat accgcgcgcc
     1201 tcgcggcgga agcgaacccc gccggcgtac gcgggttggt tgcgcgattg atgttcgcgt
     1261 acaccggtaa ctccgtgcgc actgcgcccc cgagcagcac gtaggccggc acgtcgagcg
     1321 ccttgccggc gaggtcccag agagcctgct cgatggcgct gaacgccgtc gccgcaacga
     1381 ggccctgacg cgcgagcggc tcgccctgct ggcggtagcg ttcgatctcg aacggcgagc
     1441 ggccgttgac cagatcgaag aacgccttca gctgcgatcg catccgggcg gtgtcgtcgg
     1501 acgtcgtgtt cgcgaagccg aacgcatcgg atgcttctcc gaagccgacg agtcccgcat
     1561 cggtctggag ccgcacgatg agccagacgg ttcgattggt ggctcggacg ggaagcaggt
     1621 caacgccgcg cacgcgcagc cgggggacgg cggcccgggc gccgttcaac gccaggaagg
     1681 gcgccacgac cgtgccggcc gtccgcagga cgtctcgacg actcagcttc atgccagtgg
     1741 gtcaggcctg caggacaatc gtctggccaa tctctcgaat ggcgagccgg tcctcttcct
     1801 tggcgagcgc atcctggata cgctcgatcg gctccatgaa cggctcgttg ctgagccgga
     1861 acgactggtg atggacgggg acgatcagcc gcgcacccgc cgcgtctgcc atgacgactg
     1921 cctgctccgg tgtgcagtgg ttggcgatcc acggattgta ggcgccgatc ggcatcaccg
     1981 ccgcgtcgaa ggacccgaag cgccggtgat tgccgaacag gggcgtcgct gcggtgtcgc
     2041 ccccgatcag gatcctccgt ccctcccgct cgacgacgaa gcccgtgtag ccgcgccagg
     2101 tgtcgcgccg gatacgggcg ccccagtgct tcacctcgat cgctcggacg agcacttcac
     2161 cgcgagtcgt cttcaccgtc accgattcgt tccagcgaag ctccctgact gacgagtagg
     2221 ggcgcctcgg cagaaggtcc gacgtgcccg gccccatgac ggccgccggt tgtcctcgaa
     2281 tcgcgccgag cgacgggacg tcgaggtgat cgaaatgggc gtgggacacg agaacgagat
     2341 ccacgtcggg cacgtcatcc ggtgtgagcg cgcactgcac gagacgcagc ggcccgagcg
     2401 tccccacgcc gaggtccacg ccgatgcggg gatagagcgt cgggtcggtc agaatccgca
     2461 ctccgtagaa gttgacgagc accgtcgcgt gtccgagcca cgccagcgtg acggcgttgt
     2521 ccgaccacga cgagggcgac ggcgtgtgcg gcgcggcggg aacgtcacgg ccgatttcgt
     2581 ccgcgcgctg acgcaggaat cgagcgctcc aggatgagga gagcgcagcc catgcggcgc
     2641 ccccgaggct ggcggccact cccgctgcga cgaacgctcg gcgcctcatc tagcgggccg
     2701 tatcaaccgg aagagtacga aataccacaa gcctcgccgg cgaacccgcc agccgcgaca
     2761 acggagcggc cggtcctgct tcgggaagcg caccacgagt tcatgatacg ttcgattggt
     2821 gaaggcccgc atgaatctga ggcttcctgc gcttgtactg agtttcgccc tggccctgac
     2881 gacgctcgcc gcccagcgcg acgcggatcg ccgtgattgg ctgcagttgt tcaacggcaa
     2941 ggacctggac ggctggacgc cgaagatcaa cggctatccc ctgggcgaga actacggaaa
     3001 cacgttccgc gtcgagaacg gcgtgcttgc cgtgtcctac gaccagtacg ggcagttcga
     3061 cagtaagttc ggccacctgt tctacagccg cgccgcgttc tcccattaca tcctcgccgc
     3121 cgagtaccgc ttcgttcgcg atcaggcgcc cggcggtccg tcgtgggccg tgaggaacaa
     3181 cgggctgatg ctccacagtc agccgccgca gtcgatgggc ctgaaccagg actttccgat
     3241 ctccatcgag gtccagctgc tcggcggggc gcccgcgccc gatcgcacga cggcgaacgt
     3301 gtgcacgccg ggtaccgaga tcttcatgaa gggcgccatg gttcggggcc actgcaccaa
     3361 ttccacttcc gcgatctatc gcggcgacgc gtgggtccgt gtcgaggtcg aggtgcacgg
     3421 cggcgaacgc gtcacacatc gcctcgacgg gcagacggtc ctcgaatacg agatgcttca
     3481 gatcggcggg ggctctgtca acaacttcga ccccgcggtc aagaaggacg gggcaccgct
     3541 gacccgcggc tacatcgcca ttcaaggcga gagccatccg accgagttcc ggaagatcga
     3601 attgctgaac ctttcgggat gcatgaaccc gcagtcgccg tcctacaggt cgtatttcat
     3661 agaccgggat gactcgcaat gtaggtaagg cgcggccgca ggccgtgctc gaagcccttc
     3721 gtcgtcgttt cgagctgcca ttgcccggac cggagtccca gaagcgtatg gcgccgcgcc
     3781 ttcgaagcgg gcgggagcct gccatcgatc caggcagtct gcgccccgcg gctgcattgc
     3841 tgctgatgta cccgatcgag ctcgcgtggc acataccgct tacggtacgg gcgctcgact
     3901 tgcgacatca cacgggacaa gtctcccttc caggcgggcg gatcgatccc ggcgaaaccg
     3961 tcgagggtgc ggccctgcgg gaggcggatg aggaagtcgg gctcgaacgt cgagacgtcg
     4021 tcgtgctggg ccggctgacg ccccttccca ttttcgtcag cgggcatctt ctgcatccgg
     4081 tcgtcggcat cgcggccgac cggccgcggt tcgcggtcgc cagcggggaa gtggatcgct
     4141 tgctcgagct tccagtgacg cgcctgagtc agcccgacgc cgtggcgtgg gaggaacgtg
     4201 agcgctcgag gcccccgtac ggcgtgatgg acgtccccta cttcgacgtc cacggcgcgc
     4261 gggtgtgggg cgcgaccgcg atgatccttg ccgagttcct cgacctgctt ggtgacctcg
     4321 atatctgaca cgcacgggtc ccgccggcgt gctgaactgt gcaggaataa agcccgcact
     4381 gacaccggcc gatggcactg tacgtgctgg gttgatccgg tacgcgcccg ggaggtgtca
     4441 tgggtccgct gcgggaactg atcgccgttg acgagcgccg ggcgctggag tatttcgtcg
     4501 ccggcctgca ggaggtctgc ggcaccgacg tggacaggca cgagctcctc tacaacgcga
     4561 gcgtcctggc gcactacgcg caggtctcca cccacgccga cctcgacatg cctgctccga
     4621 cagcgctcgg cgaggtcttc gatcacttcg tcatcgagtc ctcggtgccg tgcgacggcc
     4681 tgatcatgga aaccgccggc gcacagtgtc tgctgctgac cgggttcttc gagaatcaaa
     4741 tgcgcgggcg gtacaacctg cgctggtacg ccgagctcgg cgccggcttc ttcatccgcg
     4801 cggcggcgca gcagcgctcg acgcacaagg cccggctcct gcagatggtg ggaaggcact
     4861 tcgagccgtg gcggcagcgg cacgcgcggc tcggccgcga gctgcgcgat caggtgtatc
     4921 tcctgcctcc cacgcctccc gctccgttga tgtagtcagg gaacaaggcg gatgctagtc
     4981 cgccttgtgt gaggactcca tcaggaccac gcccgcgctg acaccagacg gcagcttgag
     5041 acggtccccg agggagccga gattgaatcc ctcgcggacg gtgcccttca cggtgactcg
     5101 agcaccctgc cgcgggacgc ccgcgtttga cactacccac agccgcccgg tcgtgtcgtc
     5161 gagctggtac gccccgctgc cgagcaccga gtagctgttg acgaccgagc ccgagagcgt
     5221 gacttccttg ttccggtagc ggctcgggtc cgacagaagc tggttgatgc tccgcgaggc
     5281 gcagcccgtc gtcagcgcgg cgagggcgag tatgagcagt gtcgatcggg ctctgttcat
     5341 ggcatacctg gggttcagtg tagccggatt ccacaaacgc ggcgcgcggg cgatacccgc
     5401 gcgccacgtc agtcaggtgt cagaacagga ccgcgacgtc gagcacgaac ccgcgatcgt
     5461 tgtagatcac agcgtggccg tcgatgatcc cgcgatggta gccgtacggc accgcgacca
     5521 gctcacgttc gacgtagacc ggcaccggcc gcatccgctt cgcccagcca ggcggcaggt
     5581 agccggtacg gtagtagcgg cgctgcagcc ctggaggcag cgggtggtaa tacggctggt
     5641 agtagtttcg aatcaccacg acgtcgcggt cgcggaagta acggtcgctg cggccgtagc
     5701 gatagtggtc gtcgtaccga tcctcgtagc ggacctcgac gcggcggtgc ttcgatccct
     5761 tccgccggtc accggcctcc gcagtaccgg tcaacacggt tccgctcagc accgcgccag
     5821 cgatcaagag tgagagaagc gatttcacgt tgcacctccg tgtcgaagac cggggagcct
     5881 tcgaacccct ggaagggcaa agccgttgcc aagtgcgtca gagagcgtgg aagtagaggt
     5941 attacagcgg tttagcgcgt ggcgccggat cagctgtcag ggctgaagtc gtcctcggaa
     6001 cgggacgtcc gggtcgctgc gagaggtcag cgcagtagtg tgcactacgg tgaaggggtc
     6061 tgaccctcgg tcgtcagggg agagcgaaga cccagatggc cgcgccgcgg tcgggcgggc
     6121 tcgtcaactc gggcacgagc acggcatagc tcgcggcgtg aaagccgccc tgtccgacga
     6181 cgacggccac gtactgcttg ccgtccacac tgaagctgat cgggctcgag ctgctcacgt
     6241 cgttcagccg cgtctgccac agcacctttc cagtcgcatc gtcgtaggca cggatgacgc
     6301 ggtcgaacgc gcctgcgaag acgacgccgc cggcggtggc gaggacgccg gaggtctgcg
     6361 gcgcgcgatg gcgcgtggtc cagaccgtct ggcgcgtctc caggttgatg gcctgcaggc
     6421 gcccgaagtt gccgtcgctg tctggacgtg gccggatgcc ccagttgacg cccgaggaga
     6481 gggcgccgcg tcccgctccg ccggggatcg gaaacacgtc catgcaggct tcggtcagcg
     6541 gaacgaacac cgtccggctc gtcgagttgt aggacgcggg caggtagttc ttcgcgccgg
     6601 cgccatgcgg gcagaccatc ttcagcttgc cgtcgccggg aatgaggctc gcgtcgatcg
     6661 tcttgacgcc ggtctcgggg tcgatctccg tcacgatgtt ctgcagcccg acgtcaatcg
     6721 agaaggcgaa gcgtcctgtt gccgattcga cggcgtcgaa gatgccgagc ttgccggcgg
     6781 tgaccacgag cgtgcgcgtc tgcccgcgga ctggaagccg cacgagctgc tgctcgaagg
     6841 cccagtcgag atcccattga tcgttggggt gatgctgaaa gtgccaggcg agcttgcccg
     6901 tgtccggatc gatcgcaagc gtcgagtccg tgtacagcgc atcgttggtg acgccgggcc
     6961 ggctcgaggg cggcatcagc gggccggtgt cgtaggtgtt gcccgtgccg aagtaggcga
     7021 ggccggtctc cggatcgtag cttccggtcg tccagacgga ggctccgctt cgctggctga
     7081 ttggcatgcc gttccagctg tcgccgccag gctcgccggg ctgagcgatg gtgttcacgc
     7141 gccaggcgac cgcccccgtg ccggcgtcga ggccgacgat ctgcggccca ccaggcttgg
     7201 ccccgacgcc cgtgccagtc gtgccgacca tcacctttcc cctggcggca aggacaccgc
     7261 cggcgatgcc ttcgcgaacc ttcgtgtcgc cgacaggcgt gtcccacacg agcctgccgg
     7321 tctttgcgtc gagcgcgatc acgtgcgcgt ccgacgtgcc gatgaacagg cggtctcgat
     7381 acagcgccat gccgcgcttg accgtgggag acactccctg tggcaggcgg cgcctgtact
     7441 cccacagcag gtcgccgctc ttcgcatcga gcgcctgcac gcggtcgccc atgccgtgca
     7501 cgaagagcgt tccgtcgtga accagcggga tgccctcgtt ggatccggga gggagcgacc
     7561 agctccaggc gacacgcagg ttgccgacgt tgctcttgtt gatctgtgtc agcgggctga
     7621 agccgtgcgc gtcccacgtg cggcgccagg cgagccactc ggacggcgcg gggtcgctga
     7681 gcatggcgtc agtcaccggg gtgtacttgt ccagtggcgt cggacgcacg accttcgagg
     7741 ccgaggccgc gtacggcgag taggccatga tgctgaagcc gccctgcgga atgatcatcg
     7801 aggcgagccg tccaggatcg gccggcagtt ctacggaccc tggaaggatg gcgttctgct
     7861 gaaggatgta cgcgacgatt tgtgcgtaga ctcctgcgct caacgacgcg ggcgacgtcg
     7921 tgggcatcgt gttgctggtg acggtgaaca gcgcctcgac gcttctgccg ccgtatttct
     7981 ggatgaacgg aacgcccttg agcggcggcc caaacgcgcc gtcactgagg ctcgagccgt
     8041 gacactgcgc gcagctggcg gcgtaggcgg cttcgccgag agcagcttga gcggcggtga
     8101 acgtgggcgc gcttctcgcc ggcggattac tctgctgcgc gagcggatcg ggctgggaga
     8161 ccgcgaggat cgccaccacg atgattcccc ccgccgtgat cgtccgcata ggcggagggt
     8221 agcacagtgt atgatcgggc tctgaccttc ccttggctcc gaggagtgcg agccatccca
     8281 tgattcacgt cacgaacctg gtcaagacct tcggcgaggt caaggccgtc gacgacattt
     8341 cgttcgacgt ggctgcaggc gagatattcg cgttcctcgg cccgaacggc gccggcaaga
     8401 cgaccacgat ccagatgctc acgacgctgc agcgcccgac gggcgggtcg atgcagcttg
     8461 acggcctgga ccccgtgacg cagccgctcc aggcacgcag ccgcttcggc atcgtgtttc
     8521 aggatcccag tctcgactcg gagctgaccg cgtacgagaa catggatctg cacggcgcgc
     8581 tgtaccacgt gccgaggaag atccggcgcg agcggacgga aacgctcatg aagctcttcg
     8641 agctgtggga ccgccgcgac tccatggtca agacgtattc gggcggcatg aagcggcggc
     8701 tcgagatcgc gcgcggcctg ctgcacacgc cccgcattct gttcctggac gagccgacac
     8761 tcggcctcga tccgcagagc cgcaaccagc tctggaccca cgtgaagcac ctgaacgaga
     8821 ccgagggcgt caccgtcttc ctgaccacgc actacatgga cgaggcggag cggacggcgc
     8881 accgcatcgc catcatcgat cacggccgta tcgtggcgca ggggacggcc gagtcgctca
     8941 agcagcagac gggggccgag tcgctcgagg gcgcgtttct cgcgctcacg gggaacacta
     9001 ttcgcgacga gcgcgcggat tcggcggacc agttacgcca gatagcccgc atgtggagga
     9061 gatgagccgt gaccgtcgtt tattacctgt ggctgcgtga agtaaagcgg tatctgcggt
     9121 cgcgtccgca gatcatcggc tcgatcgcgc agccgctgct gtacctgctc gtgctcggct
     9181 tcgggctcgg ccccgtgttc gcccaatccg ggcagggcag ctacctgcag ttcatcgcgc
     9241 cgggtgtcgt cggcatggcg atcctgttca cgtcgatctt ctcgggcatc gggctgttgt
     9301 gggatcgcca gttcgggttc ctcaaagaga cgctggtcgc gcccgtgcct cggctgcaga
     9361 tcatgctggg ccggacactg ggcggggcca cgatcgcgtc gattcaaggc gcgttgattc
     9421 tcgtcgtgtg tatcatcgcc ggcttccggc cgcagagcct ggccgcgctt ccgctcgcgc
     9481 tggcgttcat gctgctggtc ggcctcgtct tcgcgggact cgggaccgct gtcggctcga
     9541 cgttgaaaga catgcagggc tttcagctca tcatgaactt cctggtgatg ccgatctttt
     9601 tcctgtcggg cgcgttgttc ccgctcaaca atctgcccgc ggcgctcacc attgcgacgc
     9661 gcgccgaccc gctgtcctac gggatcgacg gcctgcgggg agccctcatc ggcgtgacgc
     9721 agtttggggt ggccacgaac ctgtcggtgc tcgcggtggt cgcctcgggc ttcctcgttc
     9781 tcggcgcctg ggcgttctcg aggatccagg tctgagggtt cgcgcgccct cggtctcgtc
     9841 ccgtcgcgcc gagcgtcggg tcctcagtgc aggagacatt aatcggcccg acgacgacct
     9901 tttgactccg gatccgtcgg atcccgagtg aatttgctgg cgttttcttt gcctgcaggc
     9961 gggcaatggc gtccgtccct gctgtcgtct cgacatccgg cgcgcccacc tcgttcccgc
    10021 gcgagggcgt cgtccgccgg gcccggcggc tctcgagcgc cgaggtcctg tggctcgcgt
    10081 tcgtgtcggt tcaggcgctc gacggggcga tgtcctacat cggcgtcacg ctgcacggac
    10141 cggcgattga ggccaacccc gtggttgcct ggtacatggg cgcattcggt cctgcggtcg
    10201 ggttcaccgc cgcgaagctg tttgccgtca tgtgcggcgt cgtcctctac gtcaccgcca
    10261 gacacaagtg ggtcgcggtt ctgacgcttc tctatctcgt cttcgccgtc ggcccctggg
    10321 cgcgcattct cgccatcagt cattagccag ttggttgcgt cgccggtcgc tcgtagctgg
    10381 cagcgcgtag ccggtagaat gccagcatgt acaccgcgct cctgatcctg cactcgtggc
    10441 ttcgatgggc ggtcatcatt gctggtctgg ccgtccttgc agggtcggtc acaggcctga
    10501 cgcgccgtcg cccgtggcag aagagcgacg atcttcgcat tcgcgtcttc acgacgagtc
    10561 tcgacgttca gtttctgatc ggcatcctcc tgtacggcgt gctgagcccg gtcacctacg
    10621 ccggcttcgc cgacatgggc gccgccatgc gcgacccgat cctccggttc tacaccgtcg
    10681 aacacatctt cggcatggtg gcggcccttg cgctggcgca catcgggcga gcgcgggtgc
    10741 ggaaggcggc cgaagcggcc cgtcaccgga cggtgctcat cttcttcggc atggcgatcg
    10801 cggtgattct gctctcgatt ccctggcccg gtacgcctgg cggccgggtt ctcttccgag
    10861 gctttgggag ttgaggcgcg ggttcctata atcagtggtc caatgcgtgt ggcgcggcgg
    10921 atcgtggtgg gcgggcgcgt gcagggtgtc gggttccgct acttcacgca agacgtcgcg
    10981 ctgcgcgaag gggtgaccgg gtgggtgcgc aacctgcccg acggccgcgt cgaggtgcac
    11041 gtcgaagggg aaaccgaggc ggtgacccgg gtcgaggccg cgatacgacg aggtccgcgc
    11101 ggggcgcgcg tgcagacggt ggacgtcgtc ggcgaggacc cgagcgggct gtacgacacg
    11161 tttgacgtga cttcataggt cgagaggtac atggatctca aacaacacat ccggcacgtc
    11221 cccgactttc ccaaggcagg cattctcttc tacgacatca cgacgcttct ccgcgatccg
    11281 aagggtttcg aggccacgat cgaccggctg tcggctccgt atgaagagga ctcgatcgat
    11341 ctggtcgtcg gcatcgagag ccgcggcttc atactgggga gcggcgtcgc ccaacgtctc
    11401 ggcgccggct tcattccgat ccgcaagccc ggcaagcttc cgtcgcatcg catcaaggag
    11461 acctacgagc tggagtacgg cacggacgcg ctcgagatcc acgccgatgc cgtccagtcc
    11521 ggccagcgcg tcctgatcgt ggacgacgtg ctggcgacgg gtgggacagc gtccgcggca
    11581 gcgaggctcg tgagacagct cgggggagac cttcatggcc tggccttcct gatcgagctg
    11641 ctctttctga agggtcagtc gaagctgccc ggcgagaagg tcttttcggt cctgcagtac
    11701 tagtgaagca tcgctcgaat acagtattgg gagtttcgta gaataggggt cgagtccccg
    11761 tagctcagat ggatagagcg cgcgcctcct aagcgcgagg ccggcagttc aattctgccc
    11821 ggggacgcca tatccagctg gtaaccggta gctggtagca ggtcacgtga ggatatgaaa
    11881 cgaaaagaac gtcaccatct gaaagagaac gagctcgtgc acaccattgg cgcggcacgg
    11941 gacttcttcg acgcgcggca gcgcgaaatc ggcgtcgtgc tcgtcgggat catcgtggtc
    12001 gcggtggtgg ccgccggcgt gatgctcttc agacagcgga accagtcacg cgggtcggat
    12061 ctgctcgccg aggcaatggt cgcgctgaac gcgcaggtcg tgccggccgg cgcgaaggcg
    12121 ggggaggaca accttccggc ggcggcgacc atcggcgcga caggcagctt ttcgacggaa
    12181 gcggcgaagt tgaccgcggc gctgccgaag ctgcaggcgg ccgccgatgc gtatcctgac
    12241 agcgccgccg gcatcaccgc gcgatatcac ctcgcaggag cgttcgccgc tcttggccgc
    12301 cacgaggacg cgatcaagca gttcgacgaa gtggccacgc gggcgggagt cggaagcgtc
    12361 tacggccgca tggcgcgcct cggaaaagcc gacgcccagg cacgggcggg acagctcgac
    12421 gccgcgatcg cgacctggaa agaaatggcg ggcgtcagct ctcctgacta tccgaccgac
    12481 gctatcctga tggagctcgc gcgcgcctac gctcagaagg gcgacaaaga ggaagcccgc
    12541 aaggcgttca cccagatcgt cgatcagcac cccgactcgc cgtacgcccc tcaggcgcgg
    12601 gcggaactcg agaacctgaa agggtaggcc gcctcgacct acggcctgcg gcggacttcc
    12661 cgcagcgcgg tgaagtcgcc cagcggaccg atgtgcagcc cgtcgagcga gcgctgcgcg
    12721 acgacgacgt tggtcctgtt ccagatggga atgtagggcg cctccagggc gatgatctgc
    12781 tgcgccctgc tgtagtagcg tttccgatcc gcctcgtcga gcgacgtcgt tgccagctcc
    12841 aggaagcgat cgacctcctc gttctggtag aggcctcgat tgaatcccga gggcggcagt
    12901 tccgaggagt ggaacacccg cctcagcatg tcgggatcca ccatcgcccc gccgacccac
    12961 tggagcgcga aaatctggaa attgcccttc agcacgtcgg cgtagaaggt cgcaaactcg
    13021 tacgacctga tgtcgagatc gatcccgacg gaacgcagat cctgctggat ggcggccgcc
    13081 tgaatccgcg tttcctcgct gttcgagatc ttgagtgaca gccggagccg cggccgcggc
    13141 cccgggccgt ccgggtccct gtagcccgcg tcctcaagca ggcgcccggc tcgctcgggg
    13201 tcgtaggtga accggtgcac atcgggctcg aaggcccacg ccagatcggg aatgaggccc
    13261 gacgcagggc ggccgagccc gcgccgcagg tactggatga ttgcgtctcg attggcggcg
    13321 tatccgatgg cgtgccggac cctcacgtcg cgcagcacgc cgtcgcgcat gttgaaaccg
    13381 aggtagaaga ggtccagtcc ggccgaacgc tggatggtga aggcgccgct cttgtcgagc
    13441 tggtacacga tgtcgggcgg aaggtcgttg acgacgagat cggtcgtccc ctttcgcagc
    13501 tcgagaccgc gcatgacgtc gtcgggcacg accttcatga cgatgccggc gttgtcgggc
    13561 gcgccgcccc agtagtcgtc gaatgcggag agcgtcagcg tgtcgtccac gtcgcggcga
    13621 acgaaccggt aggggcccgc gccgattggg cggaccttca aagagtcgcc cgcgtcggca
    13681 ggaacgatgg gcgggacgcc gaccagctgc aggggaaatg ccgcaaacgg ctccgacagc
    13741 ctgaagcgca ccgtgtagtc gtcgagcgcc gtgacctctt tcagcaggcg aaaggctccc
    13801 ttgaagggcg acacgagccc agggtcgagg acgctgcgaa acgtgtacac gacgtcgcgc
    13861 gacgtcagct cgtggccatc gtggaacctg acgccgcgcc gcaggtgcgc gatgtaggtg
    13921 agcgggtcgg ggttgtcgag ccgggaggcg agagcaggcc gaacgcgcag atcatctccg
    13981 tgctcgagga gcgggctgta gatcagctgc gacatccgct gcgtggcttc gtcgttgcca
    14041 agcctgggat cgagcgtcgt cgggccggag cgaacggcga tggtgatgat gcgtggatcg
    14101 gcgggcgcgg ccgcgcggca cccccacgcg gcgccggcaa ccagtgccac gagagccgcc
    14161 cgtcgtggcg tcatcacgtg tcttcagaca gcttcttgag ttccgccaga atctccaggg
    14221 cctgccgcgg cgtcgtgtcc tcgacgttga cggcgtcaag tcgcgcccgg atcccggcgt
    14281 cggggccagg ggccgcctcg gggcgcgcgg cgaagaggcc cagctgcgcc gtcgatgggt
    14341 ctccggcggc gccggaaagc gttggccggc cgccgcgcga ggcctcgtcg ttttcgagtc
    14401 ccgcgagaat ctgctgcgca cgcgccacga caccggccgg cagcccggcg agccttgcca
    14461 cctggatgcc gtagctgcga tcggatcggc cgctgacgat cttccgcagg aaaacgatct
    14521 gatctttcca ctcgcgggcc gtcacgtgcg cgttcgccac gcccggcagg gttcccgcaa
    14581 ggtccgtcag ctcgtggtag tgcgttgcga agagcgtctt cggtctcgcc tttgggttgc
    14641 tggcgaggta ttcggcgacc gcccacgcga tgctgaggcc gtcgaaggtc gccgtgccgc
    14701 ggccgatctc gtcgagcacg accaggcttc gcgacgtcgc cgcgtgcagg atgttggacg
    14761 tctcctgcat ctccaccatg aacgtagact ggccgcgggc gatgttgtcg gaggcgccga
    14821 cgcgcgcgaa gatccggtcc acgatcggca ccttggcgtg gcgcgccggc acgaaggaac
    14881 caatctgcgc cagaaggggg atgatcgcgg tctgccgcag gtaggttgac ttgccaccca
    14941 tgttcggccc cgtcaggatc acgagttgac gcgtggtccc gttgagatcg atgtcgttgg
    15001 gcacgaacgc gtcggtgaag cgctcgacga ccggatggcg tccgtcggtc acgaccagtt
    15061 cgtcgccgtc gtgaaggtgc ggcttggtgt agttggcaat cgtcgccgtt tcggccagcc
    15121 ccgccaggac gtcgagcgtg gcgagcgcgc gcgcggtgtc ctgaatgacg gttgactcgg
    15181 cggcgacgac gcgccgcaga tcttcgaaga tctcgagctc ccgaacgagg attcgcccgt
    15241 cggcgccgag caccttctcc tcgtattcct tgagcatcgg ggtcgtgaac cgttcgccgc
    15301 ccgcgatggt ctgctttcgc tggtagtcgg agggggccag gtgcaggttc gctttcgaga
    15361 tctcgatgta gtagccgaac acgcggttga agcggacctt gagcgaggcg atgccggtgc
    15421 gcgcgcgctc ccgctcttcc atctccgcga tcacctgcct gccggatcgg ctgatggcct
    15481 tgagctcgtc cagttcggga tcgacgccgt cgcgggtgaa gcctccctcg cgcgcaatcg
    15541 ccggcggctc gtcgatgatc gtgcgctcga tgcggccgcg caggtcgggc aggtcgtcga
    15601 gcccggcgac gaggctccgc agcaggggcg cctggagggg ctcgagcagc agcgtcaccc
    15661 gtgggaccac cgcaagcgac tggcggagcg acacgagctc gcgcggcccg gcggtgccga
    15721 gcgcaatgcg cccgatgagc cgctccagat cgtgtacacc cttgagcgcg tcgcggaact
    15781 taccgcgttc cgatgcgcga aacgcgagct cttcgaccgc gtccagtcga tcctgaatgg
    15841 gctccagcgc gcggagagga cggagcagcc aggaccgcag caggcggccg cccatcgctg
    15901 ttgccgtccg gtcgatctcg tgaagcaggg agccctggac tccgccctcg ctgccggcga
    15961 cgacctcgag gtgcttgagc gtgatggggt cgatgacgag actgtcgctc gacgtccggt
    16021 acgtgatcgc gcggacgtgg gccaggccgg ctttctgcgt ctcgcgcagg taggtcacca
    16081 gcccgccggc ggcccgcgtg gcggcaggcc ggccctccag cccgaagcct tcgaggccgt
    16141 gtgcttcgag ctgatcgagc agcgcgcgcc gcgcagcctc aggctcgaag ctccagtcgt
    16201 caacggcggt gacggcgaac tgaaggtgtg acatctcgcc gagccgcgcg gcgaccgagg
    16261 acgggtcctg aggtgcctgt ccgccgggca ccacgatctc gcgcgggcga agcacggcga
    16321 gctcgtcgcg aagcgcggag aacgcctcag ggcccacata ctccgccgcg ctgaattcac
    16381 cggttgacag gtcgatgagc gcggcgccga acgtctcggc gcctgctggt cccgacgacg
    16441 cgatggccat caggaatgct ggctccctcg cttccaggta gctcgaatcg gtcagcgttc
    16501 ccggcgagac gacgcgtacc acctcgcgcc ggacgagccc cttggccttc ttcgggtcct
    16561 cgacctgctc gcaaatggcg acgcggaatc ccttacggac gagccgcgcg atgtagccgt
    16621 cggcggcgtg gaaagggacg ccgcacatcg ggatgccgtt gccgcccgcg tccttcgacc
    16681 gcgaagtcag cgtgagctcg agcgctcgcg cggcgacgag cgcgtcctcg tagaacatct
    16741 cgtagaagtc gcccatccgg aaaaacacga tggcgtcgcg gtgctgacgc ttggcgtcga
    16801 ggtactggcg cattgccggc gtggccgtga tcgtggacga catcgttcgg gcgcattgat
    16861 tattatccag ccatgctggt cctggccctc gacaccacga caccgtcggg cagctgcgcc
    16921 cttgccgccg acggcgtcgt gcttcgcgag gaaggcagta acgcgtcccg ttcggcggcc
    16981 gcgcgcttgc ccgccgatct gatgacgata ctggagcggg cacaacggag gctcgacgag
    17041 gtcgaggtct ttgccgtcgc gacgggtccg ggatcgttca cagggcttcg catcgggatc
    17101 gcgacaatgc agggcctcgc gtttgcggca gggaagccgc tcgtcggtgt ctccggactc
    17161 gaggcgcttg cgcggatcgg gctggaagag gcggccggca ggaggatcgc cgcatgggtt
    17221 gacgcctgga gaggcgaggt ctacgccgcg ctctacgagc agggcagcga ggtcgaaccc
    17281 gtcgtcgctc gtcctgacgt gctgctgtcg caatacgccg gcgaacccac cctgttcatc
    17341 ggcgacggcg cgcgaacgta tcgagagcag atccacgcgc ggggcggccc ggcctggcgc
    17401 gtcgctgacc cggccgcgcc gatgctggcc ggtacgatcg ccactctggc cgcgtcgctg
    17461 gtcgcggcgg gcaccgcgcc tcctcctcat ctgattcggc cgctctacgt gcgtcgaacg
    17521 gacgcggagc tggcgaggca cggtcgtgcc accggatagc ccgccggagt actggatcga
    17581 tcgcctccag agcgatgcgg atctcgacgg tgtcctcgaa gtcgaggcgg agacgttcac
    17641 caatccgtgg acccgcgaga tgtacgcgtg ggagctgcag aaccgctcca tctgccacat
    17701 tttcgtcgtg cggactccgg accgtcccgt cgtcggcttt tgtgcgttct ggctggtcgt
    17761 tgacgagatt cacgtcaaca acgtggccgt cctgcccggg ctcaggggac gggggatggg
    17821 caccgccctt atgcaccggg tgttcgagga ggcggcccgg ctgggcgcgc gccgggccac
    17881 gctcgaggtc agggagtcga acgagggcgc ccgcaggtgg tacgaattcc tgggcttccg
    17941 cgttgccgga acccggcggc attactacac caaccccgtc gaagacgccc tcattctgtg
    18001 gagggaggac tctgccgggc cggcgtgacg tcaggattga gcccgggtca cacgtgtgtt
    18061 accgtgatct cacaatgcgt ttccttcttc agtaaaagga gggtgcggcc atgagcgcag
    18121 ctgaagccca ggaggtaaag aacctcctgc tgcggagcaa cgagcagtac cgccagcttt
    18181 cggaacgtca tcatcagctc gacgatcgcc tccacgaact caccgcacga ccgtatctca
    18241 cagactcaca acgattcgaa gaagtcacct tgaagaagcg gaagctcgct ctcaaggacc
    18301 agatggagca gatggcgctc gagtacgcca agacacatat ccgggcttcc tgaccgcgcg
    18361 tcgcccggtc gtcggtcccg gctcttactc atgcgaatcg atccagccgg gtggccgttc
    18421 gtcctcgggg gcagtgcgct ggcggtgctc gcctggctgc ttggcggccc ggtctatggc
    18481 gcgacgctgc tcgcgctgcc cgcgtttttc ctcttcttct tccgtgaccc ggagcggacc
    18541 catcacgcac cggcgctggg cgtggtttca cccgccgacg gccgggtcat ggtggcgggc
    18601 gcgcccacgg gcgaggccgt cccgcccggc cgatggcagc agatcagcat cttcctgtcc
    18661 ccgctcgacg tgcacgtcaa ccggataccg gtcagcgggc gcgtcactcg cgtggagtat
    18721 catcccggtc ggttcctgcc ggcctaccgt cgcgagtcgg gcgacctgaa cgagcacacc
    18781 gaagtcgtga tcgagcagcg cggccagcag gtcgtcgtcc ggcagatcgt cggcgtgctg
    18841 gctcgccgca tcgtgtgccg cacgacggaa ggcgcggagg tccgggccgg cgatcg
//
LOCUS       DBNGSH010000667         2311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2956327, whole genome shotgun sequence.
ACCESSION   DBNGSH010000667 DBNGSH010000000
VERSION     DBNGSH010000667.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2956327"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2029)
                     /locus_tag="AC16CY_15445"
     CDS             complement(<1..2029)
                     /locus_tag="AC16CY_15445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355242.1"
                     /translation="MLTRLWSLLRYRRLDREMDIELAQHLESLEAEHRARGLSPEAAR
                     LAARRDFGAVARAQEAHREQRGLPLLETLARDVRLSLRSMRRTPVVTVAVLATLAVGV
                     GANTAIFSVINAVLIRPLPYPDADRLISVSLTSRAMQIADLESAPYLYLTARDQSRTL
                     ERVGMWRVQAVNVLGGGEPQRVIALRVTADILPVLGVEPLLGRYFTAADDEPGRRPTA
                     VLMYGYWQRRFGGDPSVLGQTVVLDGSPHDIIGVMPQRFRFLDQRSVDLIAPVQLDRA
                     RVFAGGFGWPSLARLKPGTTIEQASADIARLLPIAADAFPVMPGFTRAQLDGAKWEPL
                     LLPLKQEVVGDAGNTLWVVMATLGLVLLVACANVANLILVRTESRQRELAVRAALGAS
                     WGRIARGLLTESVVFGLAGGALGVAVAYAGLRVLRTLGSSSLPRMDEIAIDRPVLLFA
                     GVVSFLSALLFGLLPVLRYARPQLSADLRSESRGSSGSREHLRARSALVVVQVALSLA
                     LLVSAGLMIRTFRELGRVDPGFMRPNAIQTLRVTIPPSSLPDPVLTARRQHAILEGIA
                     ALPGVESVAYASELPMQFGDAATDLLVTQGGSTSRGELPQNRAFHFVSPGYFATMGTS
                     IVAGRDFSWADLYEPRAVALISEDLARREWGGAAAALGKRLRGGSGLNIWRE"
     gene            complement(2039..>2311)
                     /locus_tag="AC16CY_15450"
     CDS             complement(2039..>2311)
                     /locus_tag="AC16CY_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3355243.1"
                     /translation="QHGFGLAKRILQISEGILDLNQGTLYPALVRLEQRRWIRSRWGT
                     SDNNRKAKFYELTPRGKRQLAEETESWSHTVSLMDRFLRNPAPENR"
BASE COUNT          380 a          822 c          798 g          311 t
ORIGIN      
        1 cctcgcgcca gatgttcagg cccgatccgc cacgcaggcg tttgccgagc gccgctgcgg
       61 cacctcccca ttcccgccgt gcgagatcct ccgagatcag cgcgacggcg cgtggctcgt
      121 agagatctgc ccaggagaag tctcgcccgg cgacgatcga cgtgcccatg gtggcgaaat
      181 agcccggcga cacgaagtgg aaggcgcggt tctgaggcag ctcgcctctg gacgtcgagc
      241 ctccctgcgt gacgagcagg tcggtcgcgg cgtcgccgaa ttgcatcgga agctcggagg
      301 cgtaggcgac cgactcgacg ccaggcagcg ccgcgatgcc ctccaggatc gcgtgctgcc
      361 ggcgcgccgt cagcaccggg tccggcagcg acgatggcgg gatcgtgacg cgcagcgtct
      421 ggatcgcgtt cggtcgcatg aaaccaggat ccacgcggcc cagctcgcgg aacgtccgga
      481 tcatcagtcc ggcgctgacg agcagcgcga gcgacagggc cacctgcacg acgacgagcg
      541 cgcttctcgc ccgcaggtgc tcgcggctcc cgctcgatcc gcgcgactcg gatcgcaggt
      601 ccgccgacag ttgcggccgg gcgtagcgga gaaccggcag caagccgaag agcagcgcgg
      661 ataaaaacga gacgacgccg gcaaacagca gtaccggccg gtcgattgcg atctcgtcca
      721 tccgcggcag actggacgaa ccgagagtcc gcaggacccg caggccagca tacgccacgg
      781 ccacgcccag ggcgccgccg gccaggccaa acacgacgct ctcggtcagc agcccgcgcg
      841 cgatgcgccc ccagctggcg cccagggcgg cgcgtaccgc gagctcccgc tggcggctct
      901 ctgtccggac caggatgagg ttagccacgt tggcgcacgc cacgaggagc acgagaccga
      961 gcgtcgccat cacgacccac agcgtgttgc ctgcgtcacc tacgacctcc tgcttgagcg
     1021 gcagaagcaa aggctcccac ttcgcaccgt ccagctgcgc cctcgtgaac cccggcatga
     1081 ccggaaaggc gtcggccgcg atcggcagca gacgggcaat gtccgcagac gcctgctcga
     1141 tcgtcgtgcc cggcttcagg cgcgcaaggc tcggccagcc gaagccgccg gcgaacacgc
     1201 gcgcgcgatc gagctgcacg ggcgcgatca ggtccacgga gcgctggtcg aggaagcgga
     1261 agcgctgcgg catcacgccg atgatgtcgt gcggcgaccc gtctaacacc acggtctgcc
     1321 cgagcacgga cggatcaccg ccgaaccgcc gctgccagta gccgtacatc agcaccgccg
     1381 tcggccggcg gcccggttcg tcgtcggctg ctgtgaagta ccggcccagg agcggctcga
     1441 cgccgagcac gggcaggatg tcggcggtca cccgcagtgc gatgacgcgc tgcggctccc
     1501 cgcctccgag gacgttgacc gcctgcaccc tccacatgcc gacgcgctcg agcgtgcggc
     1561 tctggtcgcg cgcggtgagg tagagatacg gagccgattc caggtcggcg atctgcatgg
     1621 ctcgagacgt cagcgacacc gagatcagcc ggtccgcgtc cggatagggc agcggcctga
     1681 tcagcacggc gttgatcacg ctgaagatgg ccgtgttcgc cccgaccccg acggcaagcg
     1741 tcgccagcac cgcgacggtg acgaccggcg tgcgccgcat cgaccggagg ctcagacgga
     1801 cgtcccgcgc cagcgtctcg agcagcggca ggccgcgctg ctcgcgatgg gcctcctgcg
     1861 cgcgggccac cgcgccgaag tcacgccggg ccgccagccg cgcggcttca ggagacagtc
     1921 cgcgtgcgcg gtgctccgcc tcgagcgatt ccaggtgctg ggccagctcg atgtccatct
     1981 cgcgatcgag ccgccggtag cgcagcaggc tccagagcct cgtcaacatg gccgggcctc
     2041 agcgattctc gggcgcgggg ttgcgcagga accggtccat gagggagacg gtgtgcgacc
     2101 agctttccgt ctcctcggcg agctggcgct ttccgcgggg cgtcagctcg tagaacttcg
     2161 ctttccggtt gttgtcggag gtcccccatc gcgaccggat ccagcggcgc tgctcgagcc
     2221 gcacgagcgc cgggtacagc gtcccctggt tgagatcgag gatgccctcg gagatctgga
     2281 ggatgcgctt cgccaggccg aagccgtgct g
//
LOCUS       DBNGSH010000668         3418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2787193, whole genome shotgun sequence.
ACCESSION   DBNGSH010000668 DBNGSH010000000
VERSION     DBNGSH010000668.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2787193"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1446)
                     /locus_tag="AC16CY_15455"
     CDS             complement(76..1446)
                     /locus_tag="AC16CY_15455"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR3355244.1"
                     /translation="MNQVVDFINVNRDRYVEELKQYLAIPSISALPEHAADVRRAAEW
                     TGDALRTAGLQNVRLIDTPGHPVVYGDWLNAPGKPTILFYGHYDVQPVDPLNLWTSPP
                     FEATVRDGEIYARGAADDKGQVFMHIKAVEAHLRKTQSLPLNMKFFIEGEEEVGSVHL
                     DDFVRSHKQDLAADVVVISDSPMFDRGIPSICYGLRGLIYFQIDLRGSKSDLHSGSFG
                     GAVANPAFVLSQLLTQMKDRGGRIKIPGFYDDVRPLSEAERAEWKKLPFNDTKYRKEL
                     GAPKLFGESGYSTLERVWARPTFEVNGLLSGFTGEGAKTVLPAVAMAKVSMRLVPDQD
                     PNKVAELFEAYLRKICPKTVELKITRMHGGKPWMTEFDNKFVRAAGRAIEQGFGQPPV
                     FNREGGSIPVVSTFQEELGLPSVLFGVGLPDENAHAPDERLDLGNFHNGIIASAYLYQ
                     EVSQLS"
     gene            complement(1443..2240)
                     /locus_tag="AC16CY_15460"
     CDS             complement(1443..2240)
                     /locus_tag="AC16CY_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355245.1"
                     /translation="MNPVPVHACNPGPMTGEGNWTWLLPGRVPTLVDAGTGEPGHLDA
                     VEQALAGDRLAQVLVTHAHGDHASGVVAIARRFEGVRFLKMFWKGRDSRWPVDWESLA
                     DGQAVAAGDETLRAVHTPGHAPDHLCFWHDASRTLFSGDLAVKGSTIYIPANLGGDLI
                     QYLASLERVLALAPSRLFPAHGPIIEDPKTVLRGYVSHRRRREEQIVAALAQADRTPD
                     ALVPIVYPGLAVHLAEVVRETILAHLLKLEREGRAGRRGDAWHMIGP"
     gene            complement(2248..2925)
                     /locus_tag="AC16CY_15465"
     CDS             complement(2248..2925)
                     /locus_tag="AC16CY_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase class-I
                     family protein"
                     /protein_id="HIR3355246.1"
                     /translation="MSAWLVHAFTASGVVLALLAARAVIENDFRAAFFWLSLQVVVDA
                     TDGLLARRARVAERLPWFDGAALDQIVDYLTYVFVPALLVWRAPLVPATWAIPVASLM
                     LLASAYGFNRTDAKTSDHFFTGWPSYWNVVTFYLLVLDWPSLVNAAVLVAFAILVFIP
                     IRYVYPSRTPILKVTTNALAAVWAILVLVMLWQYPAVSPPVLWASLVFPAYYYVLSFT
                     LHFRSGV"
     gene            complement(2953..>3418)
                     /locus_tag="AC16CY_15470"
     CDS             complement(2953..>3418)
                     /locus_tag="AC16CY_15470"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR3355247.1"
                     /translation="VPASRPLMTFADTSKPDYITPRLIAANPKARAVYDESMREAWNA
                     KNRLLELGVPLEHALYVLPNAKALRFVESGSLLALLHKWTLRTCFNAQEEIYLASMDE
                     IEQVRAVHPRIARHIGPPCVIRNGLISPRCTEGSHFCGVPVWRDFPNAVRRL"
BASE COUNT          603 a         1133 c         1145 g          537 t
ORIGIN      
        1 agaatcgcgt cgtcgctgga accgagaatc ctgatctcag aggtcacgat gaaaacgaat
       61 caggctcgtg acggctcagg agagttgtga aacctcctga tagaggtacg ccgacgcgat
      121 gatgccgttg tggaagttgc ccaggtcgag ccgctcgtcc ggcgcgtgcg cgttctcgtc
      181 cggcagcccg acgccgaaca gcacgctcgg cagcccgagc tcctcctgga acgtcgagac
      241 gaccggaata gacccgccct cccggttgaa taccggcggc tgaccgaagc cctgctcgat
      301 cgcccgcccc gcggcacgca cgaacttgtt gtcgaactcg gtcatccacg gcttgccgcc
      361 gtgcatgcgc gtgatcttca gctccaccgt cttcgggcag atcttccgga ggtaggcttc
      421 aaacagttcc gcgaccttgt tcggatcctg atcgggcacg agccgcatgc tcaccttcgc
      481 catcgccacc gcgggaagca ccgtcttggc gccctcgccc gtgaagcccg agagcaggcc
      541 gttgacctcg aaggtcggcc gtgcccacac gcgctcgagt gtcgaatatc cggactcgcc
      601 gaacagcttc ggcgccccga gctccttccg gtacttcgtg tcgttgaacg gcagcttctt
      661 ccactccgct cgctccgcct cgctgagcgg ccggacgtcg tcgtagaagc cggggatctt
      721 gattcggccg cccctgtcct tcatctgcgt gaggagctgc gacaggacga acgcggggtt
      781 ggcgaccgcg ccgccgaacg atccggagtg cagatcgctc ttggagcccc gcaggtcaat
      841 ctgaaagtaa atcagcccgc gaaggccgta gcagatcgac gggatgccac ggtcgaacat
      901 cggcgagtcc gagatgacga cgacgtcggc ggcgagatcc tgcttgtggg atcggacgaa
      961 gtcgtccagg tgaacgctgc cgacctcttc ctcgccctcg atgaagaact tcatgttgag
     1021 cggcaggctc tgcgtcttgc gcaggtgcgc ttccaccgcc ttgatgtgca tgaacacctg
     1081 ccccttgtcg tcggcggcgc cgcgcgcgta aatctcgccg tcgcgcacgg tggcctcgaa
     1141 aggcgggctc gtccagaggt tcagcggatc cacgggctgc acgtcgtagt gcccgtagaa
     1201 caggatcgtc ggctttcccg gggcgttgag ccagtcgccg tagacgaccg ggtggcccgg
     1261 cgtgtcgatg agccgcacgt tctgcaggcc tgccgtcctg agcgcgtcgc cggtccactc
     1321 ggccgcgcgg cgcacgtcgg ccgcatgctc gggcaacgcg ctgatgctgg gtatcgcgag
     1381 gtactgcttg agctcttcga cgtacctgtc gcggttcacg ttgatgaagt cgacgacctg
     1441 gttcatggcc caatcatatg ccacgcgtcg ccgcgccgcc ccgcgcgccc ctcacgttcg
     1501 agcttcagca ggtgggcgag gatcgtctct cgcacgacct cggcgaggtg cacggcgagg
     1561 ccgggataga cgatcggcac gagcgcgtca ggcgtgcggt cggcctgggc gagcgcggcc
     1621 acgatttgct cctcgcgccg gcggcgatgc gagacatagc cgcgcagcac cgtcttcggg
     1681 tcctcgatga tcggcccgtg cgccggaaag aggcgcgacg gggcgagcgc cagcacgcgt
     1741 tcgagcgagg ccaggtactg gatgagatct cctccaaggt tggcggggat gtagatggtc
     1801 gagcccttga cggcgaggtc gccagagaag agcgtccgcg acgcgtcgtg ccagaagcac
     1861 aggtggtccg gcgcatggcc tggcgtgtgg accgcgcgga gagtctcgtc ccctgcggca
     1921 accgcctgcc cgtctgccag gctctcccag tccacaggcc agcggctgtc gcgccccttc
     1981 cagaacatct tgaggaaccg cacgccctcg aagcggcggg cgatcgccac gacgcccgat
     2041 gcgtggtcgc cgtgagcgtg cgtcacgagc acctgtgcga gccgatcccc cgcgagcgcc
     2101 tgctcgacgg cgtcgagatg ccccggttcg cccgtcccgg cgtcaacgag cgtcggcacg
     2161 cgcccgggaa gcagccacgt ccagttgccc tcgccggtca tcgggccagg gttgcacgcg
     2221 tgcacgggga cgggattcat ggcggggtca gacccctgaa cggaaatgca gcgtgaacga
     2281 caggacgtag tagtacgcgg gaaacaccag agaggcccag agcacaggcg gcgataccgc
     2341 gggatactgc cacagcatca ccaataccag gatcgcccag acggccgcga gcgcgttggt
     2401 cgtgaccttg aggatcggcg ttcgcgacgg atagacatag cggatcggaa tgaacaccag
     2461 gatcgcgaac gccaccagca ccgcggcgtt gacgagagac ggccagtcca ggacgagcag
     2521 gtagaacgtc acgacgttcc agtacgacgg ccagcccgtg aagaagtgat cgctcgtctt
     2581 ggcgtcggtg cggttgaagc cgtacgcgct cgcgagcaac atcagcgacg ccacggggat
     2641 cgcccacgtg gccggcacga gcggcgcgcg ccagacgagg agcgcgggca cgaagacgta
     2701 ggtcaggtag tcgacgatct ggtcgagcgc ggcgccgtcg aaccacggca ggcgctcggc
     2761 cacgcgggca cgcctcgcga gcaggccgtc ggtcgcgtcg acgaccacct gcagcgacag
     2821 ccagaagaaa gcggcgcgga aatcgttctc gatgaccgcc cgggccgcca gcagcgccag
     2881 cacgacgccg ctcgccgtga aggcatggac caaccaggcg ctcatcgcaa caagctggtg
     2941 aaagttagaa gctcacagtc tccgtaccgc gtttggaaag tctctccaga ccggcacgcc
     3001 gcagaagtgc gacccctcgg tgcagcgggg cgagatgagg ccgttgcgga tgacgcaggg
     3061 cgggccgatg tggcgggcaa tccgcggatg cacggcgcgc acctgctcga tctcgtccat
     3121 cgacgcgagg tagatctcct cctgcgcgtt gaagcaggtc cggagcgtcc acttgtgcag
     3181 cagcgccagc agcgaaccgg actcgacgaa ccgcagcgcc ttggcgttgg gcagaacgta
     3241 cagcgcgtgc tcgagcggga cacccagctc gagcagccgg ttcttcgcgt tccacgcttc
     3301 ccgcatcgac tcgtcgtaca ccgccctggc cttcgggttg gcggcaatca gccgcggcgt
     3361 gatgtagtcg ggtttcgacg tgtcggcaaa cgtcatgagc ggccgcgacg caggcacc
//
LOCUS       DBNGSH010000669         2241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2787466, whole genome shotgun sequence.
ACCESSION   DBNGSH010000669 DBNGSH010000000
VERSION     DBNGSH010000669.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2787466"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="AC16CY_15475"
     CDS             complement(<1..371)
                     /locus_tag="AC16CY_15475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3355248.1"
                     /translation="MADRHVVIIGSGPAGLTAALYTARANLKPLLIEGLEAGGQLMLT
                     TMVENWPGFHDGIMGPDLMAEMRKQAERFGTDMVQGNVSKVELKHRPFTISFDGGQKV
                     TTDALIIATGASARWLEIGSDR"
     gene            complement(528..860)
                     /gene="trxA"
                     /locus_tag="AC16CY_15480"
     CDS             complement(528..860)
                     /gene="trxA"
                     /locus_tag="AC16CY_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3355249.1"
                     /translation="MASDKVRTFTDNNFDDETGKGLVLVDFWADWCGPCRRLAPTVDA
                     LASEFDGRATVAKLNVDENPEVPRRFSVRGIPTLLLFKDGQLAETIVGLRAKEDIAKM
                     MERHLGRQ"
     gene            complement(901..>2241)
                     /locus_tag="AC16CY_15485"
     CDS             complement(901..>2241)
                     /locus_tag="AC16CY_15485"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR3355250.1"
                     /translation="DVVMPRYRHVKAGALIGQLRVVHGGQVDQVDLFAAVENRVRTVF
                     VSHAAYFDRDYLYGVASHDYPDNPERFAFAGQAALTWARSTGQRYDVVHAHDWQAGLV
                     PLQLQRMAPEWSDSARTARVFTIHNIAYQGVFDASWLPRLGLGWDLMRVDALEYWSRI
                     SFLKGGVVFSDLVTTVSPTYAREIQSPELGFGFDGILRARSSDLVGILNGIDYDQWDP
                     ERDLNLPVPFSASKLAGKGTAKRRVLETFGLPTTPDVKRRPLVAMISRMVDQKGFDLL
                     DQISEKLPALGATFVLLGSGEPRYETAWRDLAARHPARIGVRIGFDEGLAHLVEGGAD
                     LFLMPSRFEPCGLNQMYSLRYGTVPVVHRTGGLADTVRDVDARSGTGTGFVFREYSGR
                     ALLAALTRGIEMFENRPGWRRIQKAGMREDFSWDASARDYVEVYDRAIRRRAGN"
BASE COUNT          392 a          761 c          699 g          389 t
ORIGIN      
        1 cggtcggatc cgatctcgag ccagcgcgcc gaggcccctg tagcgatgat cagcgcgtcg
       61 gtcgtcacct tctgcccgcc gtcgaacgag atcgtgaagg gacggtgctt cagctcgact
      121 ttcgagacgt tgccttgcac catgtcggtg ccgaagcgct ccgcctgctt gcgcatctcg
      181 gccatcaggt ctggacccat gatgccgtcg tggaagcccg gccagttctc caccatcgtg
      241 gtgagcatca actggccgcc cgcctccaat ccctcgatga ggagcggctt caggttggcg
      301 cgagccgtgt agagggccgc cgtgaggccg gccgggccgg aaccgatgat gacaacgtgt
      361 cgatcggcca ctctagacat ctccaaaggc tgacgtcgtt gcgaatgaag caggtgtctc
      421 cctgcctttg gagatgtcta gccccgcgga gcggggccag ccaacgccgt cggcagtgaa
      481 gcggaggggc gaccgctcac gtggtctcgg tggttggatg cgtagcttca ctgccgtccc
      541 aggtgccgtt ccatcatctt ggcgatgtct tccttcgcgc ggagaccgac gatcgtctcc
      601 gccagttgtc cgtccttgaa caggagcaaa gtgggaatgc cgcgcaccga gaaccgcctc
      661 ggaacctcgg ggttctcatc cacgttcagt ttggccaccg tcgcgcggcc gtcgaattcc
      721 gatgcgagcg cgtcgacggt cggagccaga cgccggcagg gaccgcacca gtccgcccag
      781 aagtctacga gcaccaggcc cttgccggtc tcgtcgtcga agttgttgtc ggtgaacgtt
      841 ctcactttgt cggatgccat cagttctcat ctccacggac tagtggacgg taagtcagcc
      901 tcaattcccg gcgcggcgcc ggatcgcgcg gtcgtacacc tcgacgtagt cccgagccga
      961 ggcgtcccag gaaaaatcct cacgcatgcc ggccttctgg attcttctcc acccaggccg
     1021 gttctcgaac atctcgatcc cgcgagtaag cgccgccagc agcgcgcgcc ccgagtactc
     1081 gcgaaagacg aatcccgtgc ccgtccccga gcgggcatcg acgtcgcgca ccgtgtcggc
     1141 gagtccgccc gtccggtgca cgaccggcac cgtcccgtag cgcaggctgt acatctggtt
     1201 cagcccgcac ggctcgaacc gcgacggcat caggaacagg tcggcgccgc cctcgaccag
     1261 atgcgccagc ccctcgtcga atccgatcct gactccgatc cgggccggat gccgagccgc
     1321 gaggtcgcgc cacgcggtct cgtagcgcgg ctcgccggac ccgagaagga cgaacgtcgc
     1381 ccccagcgcc ggcagctttt ctgagatctg atcgagcagg tcgaagccct tctggtcgac
     1441 cattcgcgaa atcatcgcga ccagcggtcg gcgtttcacg tccggcgtgg tgggcaagcc
     1501 gaatgtctcc aggacccttc gcttggcagt ccctttccct gccagtttgg acgcactgaa
     1561 tggcaccgga aggttcaagt cgcgctcggg atcccattga tcgtaatcga tcccgttcag
     1621 aatgccgacc aggtcgctcg aacgggcccg gagaatcccg tcgaacccaa agcccagctc
     1681 gggactctgg atttcccgcg cgtaggtagg gctgacggtg gtgaccaggt cgctgaacac
     1741 gaccccgccc ttgagaaagc tgatccggct ccaatactcc aacgcgtcca cgcgcatcag
     1801 atcccacccc agccccagcc gcggaagcca gctggcgtcg aagacaccct gataggcgat
     1861 gttgtgaatc gtgaacaccc tggcagtccg cgccgagtcg gaccattccg gcgccatgcg
     1921 ctggagctgc agcggtacga gaccggcctg ccagtcgtgg gcgtgaacga cgtcgtagcg
     1981 ctgtccggtc gaacgcgccc acgtcagcgc ggcctgaccg gcgaacgcga agcgctcggg
     2041 attgtcggga tagtcgtgag aggcgacgcc gtacaggtag tcgcgatcga agtacgcggc
     2101 gtgcgatacg aacaccgttc gaacgcgatt ctcgacggcc gcaaacaggt ccacctggtc
     2161 cacctgcccg ccgtggacga cccggagctg tccgatgagg gcgccggcct tgacgtgccg
     2221 gtaccggggc atgaccacgt c
//
LOCUS       DBNGSH010000670         5509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_750264, whole genome shotgun sequence.
ACCESSION   DBNGSH010000670 DBNGSH010000000
VERSION     DBNGSH010000670.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_750264"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="AC16CY_15490"
     CDS             <1..314
                     /locus_tag="AC16CY_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355251.1"
                     /translation="SIKPLGRLFPVIWFGFWINLFTGVSIFLKDASIYGRNWDLYIKL
                     FFVAAGVVLLGITRKQVFGAGDGPIPPQSKALAWASLACWFCAIVAGRLIAYVGPVPG
                     L"
     gene            640..1569
                     /locus_tag="AC16CY_15495"
     CDS             640..1569
                     /locus_tag="AC16CY_15495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3355252.1"
                     /translation="MKRRQFLQRALLLGAGAALGTKAAAQGGTPRVSFTFDDFSIVDA
                     PMLSGAARNQAILDALQAQGLSAAAFVIGRNVEQPETMRLLEAWSGAGHIVGNHTYSH
                     RRYSRVPFGDFTDDVLRCEALLRRLPSFRKLLRFPFLDEGATTEQRDRIRAFLVERGY
                     GNAHVTIDASDWYVDGRLRARLERQRDADPAPYGRFYVDHIWERAQFYDGLSRQLLSR
                     SVNHTVLLHHNMLNALFLGELIQMFKSKGWSVVDVRNAYEDPVFRLAPDVLPAGQSLL
                     WSLAKATGRFDGILRYPGEDGTYEASKMDALGL"
     gene            complement(1639..2124)
                     /locus_tag="AC16CY_15500"
     CDS             complement(1639..2124)
                     /locus_tag="AC16CY_15500"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355253.1"
                     /translation="MKIEMTTSSSRQSMSGRLNGCGGTRWRLSLATALAVGALLGASP
                     TLAHHSRAMFDVGKNVTYRGVVKDYRWQNPHSHIVVTVGAGATDPSTVGTWTVEASAI
                     NTMTSRGWTPKTYKPGDPITVVAHPNMDGSTVVLLFYAIKADGTRLYRATHRYPGEEE
                     R"
     gene            2466..4631
                     /locus_tag="AC16CY_15505"
     CDS             2466..4631
                     /locus_tag="AC16CY_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3355254.1"
                     /translation="MSFAAWGLVGGLASVFAELYSLTASETALLVAVPVLLGSLARLP
                     MGMLTDRYGGRAMFTLLLVASAFACWLVPLTTTYRSLVTAAFFMGLAGSSFAIGVGFV
                     SRWTPADRQGTALGLYGLGTAGQSAAVFGGPLIAGWLGWEATFRGMGLLLLLWAAVFA
                     ALARNSGRTTTAAGVSAMIRVLRHEPIAWLLGGFYFLTFGGFVAFSIYLPTLLRAQFG
                     LSPGDAGFRAAGFVVLATAMRPAGGWLADRIGGAQVMSWVLGGVALFAMLLSWPAMVP
                     FTVGALGCAVLMGLGNGAVFKLVPERFPTQTGTVTGLVGALGGLGGFFPPLLLGAFRD
                     TIGVLWPGFALLSATALVLRVANRRVFRPADVAWRLALPAAARDRVDRFQAGAWATLF
                     TLALGAAIVVGSRNLKNFDAALVGYTFATLFAAFGISYRYAMWLQRPPTRMYWRRGWQ
                     MFTARGFLGHNAAALSRRALVDFAGNRFIFRRGRLRGVAHWMIMWGCLLAAAITFPLV
                     WGWIHFETVPGSLEMYRAYVYGFPAYDFHVESLFAFLMFHGLVWASFLVIAGVMLAFR
                     RRMIDHGAVAVQQFGQDVLPLLLLFAISVTGLMLTASYTWMRGYAYDFLAILHAATVI
                     LTLLWLPFGKLFHVFQRPAQLGVSFYKDAGERGAQASCARCGQAYASLTMVQDLMAVE
                     RDLGFSYETTDGGHYQQICPRCRRALFGLAQGALWRASR"
     gene            4639..>5509
                     /locus_tag="AC16CY_15510"
     CDS             4639..>5509
                     /locus_tag="AC16CY_15510"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR3355255.1"
                     /translation="MATIPVDELRIIDRYGPHLSSMTGARLSTSVEPEKLVKTHCCFC
                     GQQCGIQLKVRGNEVIGFEPWEDFPFNRGMLCPKGVKRYLQGSHPDRLLSALARDPSS
                     PSGFTPLPYEQAIARVSSEIDRIQRMFGSSAVGVLSGASLTTEKTYLLGKFARVCLKT
                     PFIDYNGRLCMVSAGAANKKAFGIDRTTNPWSDMIGTDVIWVAGSNVAECSPITTNYV
                     WQAREQGARIIMQDPRITPAARTCDLYLPVKPGRDAALFAGVLRIMIERYWLDHAFID
                     AHTTGFDQVADYCR"
BASE COUNT          809 a         1817 c         1818 g         1065 t
ORIGIN      
        1 tctccatcaa gccgctcggg cggctcttcc ccgtcatctg gttcggtttc tggatcaacc
       61 tcttcacggg cgtgtcgatc ttcctgaagg acgcgagcat ctacggccgc aactgggacc
      121 tgtacatcaa gctgttcttc gtggcggccg gcgtggtgct gctgggcatc acgcgcaagc
      181 aggtgttcgg cgccggcgac ggtccgatcc cgccacagtc gaaggcgctg gcgtgggcgt
      241 cgctcgcctg ctggttttgc gcgatcgtcg cgggccgctt gatcgcctac gtcggcccgg
      301 tgcctggtct gtaagaagac cggtcaacgt ccgacgccca actcccaacg cccaactccc
      361 aacagtgtcc ggcttccttg ggcgccaaag cgcgcagcgc gaaggcggca gccggaccaa
      421 acggctcgga ctcgacgcgc acctgctgcg aatcccgcac ggatcgacaa acactacgcg
      481 actgctcgtt acgttgggct tcgcccgatg gctgggtgct gcggccgttg ggggatgtag
      541 gatcctgccc tgggctttcc atgccgatcg cctgtgcccc ggcgcagttg cggcccgtgt
      601 ccaacgtcag gtcagcggag gcgactcgac aacgtgggga tgaagcgacg ccagttctta
      661 cagagggcgc tcttgttagg tgccggtgcg gcccttggga cgaaagcggc cgcccaaggc
      721 gggacgcccc gagtcagttt caccttcgac gacttcagta tcgttgatgc gccgatgctt
      781 tcgggagcgg cacgaaatca agcgatcttg gacgcgttgc aggcgcaggg cctgagcgct
      841 gcggcgttcg tgatcggacg gaacgtcgag cagcccgaga cgatgcggct actggaggcg
      901 tggagcggcg ccggccacat cgtcggcaac cacacctatt cgcatcggcg gtacagccgc
      961 gtcccgtttg gcgacttcac cgacgacgtc ctccgatgcg aggcgctgct tcgtcgactg
     1021 ccgagttttc ggaagctgct tcggttcccc ttcctagacg agggtgccac gactgagcag
     1081 cgagaccgta tccgggcgtt tctcgtcgag cggggctacg gcaatgccca tgtcacgatc
     1141 gacgcgtcgg actggtacgt ggacggccga ctgcgggcgc gactcgaacg tcaacgtgac
     1201 gcggacccag cgccttacgg caggttttat gtggaccaca tctgggagcg ggcgcagttc
     1261 tacgacggcc tgtcgcggca gctgctcagt cgcagcgtga accacacagt cctgctccat
     1321 cacaacatgt tgaacgcctt gttccttgga gaactgattc agatgttcaa gagcaaggga
     1381 tggtcggttg tcgacgttcg caacgcctac gaggatccgg tctttcgatt ggcgcctgat
     1441 gtgttgcccg cgggtcagag tttgttgtgg tcgctggcca aagcgacagg ccgcttcgac
     1501 ggtatcctgc gatacccagg ggaggacggc acgtacgaag catcgaagat ggatgcactg
     1561 ggcctctgat tggccgtcga ctgggcagca cctgcatccc gcgccgcctc tgggctgttc
     1621 gctacgttcg gctcgccgtt accgttcttc ttccccagga taacgatgcg tggcgcgata
     1681 caaccgcgtt ccgtcagcct taatggcgta gaagagcaac acaacggtgg agccgtccat
     1741 gtttgggtga gccaccaccg taatcgggtc gcccggcttg tacgtcttcg gagtccaccc
     1801 ccttgaggtc atggtgttga tagcggaggc ctcgacagtc cacgtcccca ccgtagacgg
     1861 gtccgtcgcc ccagccccaa cggtgacgac gatgtggctg tgtgggttct gccaccggta
     1921 gtcctttacg actccccgat atgtgacgtt cttgcccacg tcgaacatcg cgcgggaatg
     1981 gtgggcgagt gtcggactag ctcccaacaa agctccgacc gccagggccg tagccagcga
     2041 cagccgccat ctggtaccgc cgcatccatt cagtcttccg ctcatgctct gccgggagga
     2101 actggtggtc atctcaatct tcaaagaatc ctagtccagg tcaagggggg gtgagtgatg
     2161 aaatacggcg tagtggcgaa tgcgctcaag gcaggcgtcg attttcgaca caccggaaag
     2221 tcctcagcgc gcccgacaca ggggcgcttc acttccgcct ccgtgtctcc gtggttcgtt
     2281 ggcgatcctg gcgggccgct tgatggcctt cacgcgagtc actgcggctt ccacacgagc
     2341 acggaatacg ggctccggga tctcccttga cagggacccg atcggccgga tcagactagc
     2401 cccgtcgggg tgcatctctg agagggtctc gatcggcgct cgccctcgcc acgatctcct
     2461 tcgcgttgtc gttcgccgcg tgggggctcg tcggcggtct cgcctcggtg ttcgccgagc
     2521 tctatagcct caccgcgtct gaaacggcgc tgctcgtcgc ggttcccgtt ctgctcggct
     2581 cgctcgcccg cctgcccatg ggcatgctca cggatcgata cggcggccgc gcgatgttca
     2641 cgctgctgct cgtggcgtct gctttcgcct gctggctcgt gccgctgacg accacgtatc
     2701 gcagcctcgt gacagcggcg tttttcatgg gcctcgcggg atcctccttc gccatcggcg
     2761 tggggttcgt gtcgcgatgg acgccggccg atcggcaggg caccgcactc ggcctctacg
     2821 gcctgggcac ggcaggccag tcggctgcgg tgttcggcgg tccgctgatc gcgggctggc
     2881 tgggctggga agcgacgttc agaggcatgg gcctgctgct cctgctatgg gccgcggtct
     2941 tcgccgccct cgcgcgcaac agcggccgga cgacgaccgc ggccggcgtg agcgccatga
     3001 tccgcgtact ccggcacgag ccgatcgcgt ggctgctcgg cggcttctac ttcctgacct
     3061 tcggcggctt cgtcgcgttc tcgatctacc tgccgacgct gctgcgagcg cagttcggcc
     3121 tgagccccgg cgatgcgggc ttccgcgcgg ccggcttcgt cgtgctggcg acggcgatgc
     3181 ggcccgccgg cggctggctc gccgatcgca tcggcggcgc gcaagtcatg tcctgggtgc
     3241 tcggcggcgt ggccctgttc gcgatgctcc tgtcatggcc ggccatggtg ccgttcacgg
     3301 tgggcgcgct cgggtgcgcc gtgctgatgg ggctcggcaa cggcgcagtc ttcaagctgg
     3361 tccctgagcg ctttccaacc cagaccggca cggtcaccgg actggtcggc gcgctcggcg
     3421 gcctcggcgg gtttttcccc ccgctgctgc tcggcgcgtt ccgcgacacc atcggtgtgc
     3481 tgtggcccgg cttcgcgctg ctctcggcga cggccctggt cctgcgcgtc gcgaatcggc
     3541 gcgtgttcag gccggccgac gtcgcctggc ggctggcact gccggcggcg gcgcgcgacc
     3601 gggtcgatcg ctttcaggcc ggcgcctggg ccacgctctt caccctcgca ctgggcgcgg
     3661 ctatcgtcgt cggctcgcgc aatctgaaga acttcgacgc ggcgctcgtc ggctacacgt
     3721 tcgcgacgct gtttgccgcg ttcggcatct cctaccgtta cgccatgtgg ctgcagcggc
     3781 ccccgacgcg gatgtactgg cggagagggt ggcagatgtt cacggcccgc ggtttcctgg
     3841 ggcacaacgc ggcggccctc tcccgccgcg cgctcgtcga tttcgccggc aaccgtttca
     3901 tcttccgccg gggacgcctg cggggggtcg cgcactggat gatcatgtgg ggctgccttc
     3961 tggccgcggc gatcaccttt ccgctcgtct ggggctggat ccatttcgag acggtcccgg
     4021 gcagcctcga gatgtatcgc gcctacgtgt acggattccc ggcgtacgac tttcacgtgg
     4081 aatcgctctt cgccttcctc atgttccacg gcctggtgtg ggcgtcgttc ctcgtcattg
     4141 cgggcgtgat gctggcgttc cgccgccgga tgatcgacca cggagcggtc gcggtgcagc
     4201 agttcggcca ggacgtcctg ccgttgctcc tgctcttcgc aatcagcgtc acagggctca
     4261 tgctgacggc gagctacacc tggatgcggg ggtacgccta cgatttcctt gccatcctcc
     4321 acgccgccac ggtgattctg accctgctgt ggctgccatt cggcaagctg ttccacgtgt
     4381 tccagcgtcc cgcgcagctg ggcgtgagct tctacaagga cgccggcgaa cgaggcgcgc
     4441 aggcgagctg cgcccgctgc ggccaggcgt atgcgtcgct gacgatggtt caggacttga
     4501 tggcggtcga gcgggatctc gggttcagct acgagaccac ggacggcggg cattaccagc
     4561 aaatctgtcc gaggtgccgc cgggctctct tcggccttgc gcaaggcgcg ctctggcgcg
     4621 cgagccggta atcgaatcat ggccaccatc cccgtcgacg aactgagaat catcgatcgg
     4681 tacggaccgc acctgtcgtc gatgacgggc gcgcgcctgt cgacgagcgt ggagcccgag
     4741 aagctggtca agacgcactg ctgcttctgt ggtcagcagt gcggcatcca gctcaaggtc
     4801 cgcggcaacg aggtgatcgg cttcgaaccg tgggaggact tccccttcaa ccgcggcatg
     4861 ctgtgcccca agggagtcaa acggtacctg cagggctcgc acccggaccg tctgctcagc
     4921 gcgctggcgc gggacccgtc gtcgccatcg ggcttcacgc cgcttccgta cgagcaggcc
     4981 atcgcacgcg tgtcgagcga gatcgaccgg atccagcgga tgttcggatc gagcgccgtc
     5041 ggcgtcctca gcggcgccag cctgaccacc gagaagacct acctgctcgg gaagttcgcc
     5101 cgcgtgtgcc tgaagacgcc gttcatcgac tacaacggcc ggctctgcat ggtgagcgcg
     5161 ggggccgcca acaagaaggc gttcggcatc gaccggacga cgaacccgtg gtccgacatg
     5221 atcggtacgg acgtgatctg ggttgccggc agcaacgtcg ccgagtgctc gcctattacg
     5281 acgaactatg tgtggcaggc tcgcgagcag ggcgcacgga tcatcatgca ggacccgcga
     5341 atcacgccgg ccgcccggac gtgcgacctg tacctgcccg tcaagccggg acgcgacgcc
     5401 gcgctcttcg cgggcgtgct caggatcatg atcgagcggt actggctcga tcacgctttc
     5461 atcgacgcgc acacgacggg gttcgaccag gttgccgact actgccggc
//
LOCUS       DBNGSH010000671         2059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_410356, whole genome shotgun sequence.
ACCESSION   DBNGSH010000671 DBNGSH010000000
VERSION     DBNGSH010000671.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_410356"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="AC16CY_15515"
     CDS             complement(<1..967)
                     /locus_tag="AC16CY_15515"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355256.1"
                     /translation="MLKATAGKILPTTITGSLPRPSWYTENLGTRSFLDAMVISRFRE
                     QYVDALSVYLREQEVAGLDIVTDGDCRFDQDIGGQSWTSYPPHHMSGFETTHPKLAKV
                     GAGGIAFPRGHILHDYLEARVMPKLVGAVGRGEMQYAELFKAAQRLTTKPVKFGTVTP
                     ELVAFAVQDEHYQDLPSRIMALSDAFNAELHDLADAGCQVIQMEEPQIHLLAARGYVD
                     KVINPEFMLKVFNNTVKGLRAKTEVWCHTCWGNPSQQRMFGSVQSYKPALTLLNQVDA
                     DVITFEMKSSNALDLEDVGKAITNMKVCIGAIDHHTLQVESPGEVA"
     gene            complement(1100..>2059)
                     /locus_tag="AC16CY_15520"
     CDS             complement(1100..>2059)
                     /locus_tag="AC16CY_15520"
                     /inference="COORDINATES: protein motif:HMM:NF017257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HIR3355257.1"
                     /translation="LNNGVPDGQSDYRFGSLPLGGVFPEQNARGYGGEIQASMAAFGA
                     MFGTSPRGFLNETWTGGLRLGSPDAPVRLIAARDLVKDSMLSYAGARDPGTGTRWGGV
                     VSNSVFLHFNRDESGDGQYLSVGGGSLRGVNVDDNWNAAAGAGAYWTIGTGDQANLTL
                     GVDLTGMHYAKNLSFFTLGHGGYFSPQLYFMASVPVRWNIKRAGGMYEIRISPGIQHF
                     YEASSPLYPTGAILTPPEPFAPMVYPEQTRTAPNYDIAFRADYRLTPHLHLVAFANAN
                     NTRDFNQQVFGIALKVLGQRLPATRELNVKAIPDWRGNQPLGN"
BASE COUNT          352 a          675 c          641 g          391 t
ORIGIN      
        1 cggccacttc gcccggcgac tccacctgca gcgtgtggtg atcgatcgcg ccgatgcaca
       61 ccttcatatt ggtgatcgcc ttcccgacgt cctcgaggtc gagcgcgttc gagctcttca
      121 tctcgaaggt gatcacgtcc gcgtccacct gattgaggag cgtcagtgcc ggcttgtagc
      181 tctggacgct gccgaacatc cgctgctggg aggggttgcc ccagcacgtg tggcaccaga
      241 cctccgtctt ggcacgaagt cccttcaccg tgttgttgaa gaccttgagc atgaactcgg
      301 ggttgatgac cttgtccacg tacccgcgcg cggcgagcag atggatctgc ggctcctcca
      361 tctgaatgac ctggcagccc gcgtcggcga gatcgtgcag ctcggcgttg aaggcgtcgc
      421 tcagcgccat gatgcgcgag ggcaggtcct gatagtgctc gtcctgcacg gcgaaggcga
      481 cgagctcggg cgtcaccgtc ccgaacttca cgggcttggt cgtcaggcgc tgcgccgcct
      541 tgaacagctc ggcgtactgc atctcgccgc gccccaccgc gccaaccagc ttcggcatca
      601 cgcgcgcttc gaggtagtcg tgcaggatgt gcccgcgtgg aaacgcgatg ccgccggcgc
      661 ccaccttggc gagcttcgga tgtgtcgtct cgaagccgct catgtggtgc ggcggatagc
      721 tcgtccagct ctgccccccg atgtcctggt cgaaccggca gtcgccgtcg gtcacgatgt
      781 cgagcccggc cacctcctgc tcccgcagat acaccgacag cgcgtccacg tactgttccc
      841 ggaaccgcga tatcaccatc gcgtcgagaa acgatcgggt ccccaggttc tccgtgtacc
      901 aactcggacg cggcaacgac cccgtgatgg tcgtcggcag tatctttcca gcggtcgcct
      961 tcagcatgaa agagtctcca tcaatggata caagctttga acgttacaat gccggactct
     1021 ttcagtcaac gaagcgccgg cgcgccacgc atgcgcgcgg gtgacttttc gcagagggaa
     1081 acggacggcg ggtccgatct cagtttccga gtggctggtt gccgcgccaa tcgggaatcg
     1141 ctttcacgtt caactccctc gttgcgggca ggcgctgtcc cagcaccttg agggcgatcc
     1201 cgaagacctg ctggttgaag tcgcgcgtgt tgttggcgtt ggcgaacgcg accagatgca
     1261 ggtgaggcgt cagccggtag tcggcccgga acgcgatgtc gtagttgggt gcggttctcg
     1321 tctgctcggg gtagaccatg ggcgcgaacg gctccggcgg cgtcaggatc gcgccggtcg
     1381 gatagagcgg cgatgaggct tcgtagaagt gctgaatgcc aggactgatc cggatctcgt
     1441 acatcccgcc ggcgcgcttg atgttccagc gcacgggcac cgacgccatg aaatacagct
     1501 gcgggctgaa gtaccccccg tgtccgagcg tgaagaaact caggttcttg gcgtagtgca
     1561 ttcccgtcag gtcaaccccg agcgtgagat tcgcctgatc tccggtcccg atcgtccaat
     1621 aggcgcccgc accggccgcc gcattccagt tgtcgtccac gttcacgccg cggaggctcc
     1681 cgccgccgac ggacaagtac tgaccgtctc cggactcgtc gcgattgaaa tgcagaaaga
     1741 ccgagttgga gacgacgcca ccccagcgag tgcccgtacc cgggtcgcgc gcaccggcgt
     1801 acgacaacat gctgtccttg acgagatccc tcgcggcaat cagtctcacc ggcgcgtcgg
     1861 gactgccgag cctcaagccg cctgtccacg tctcgttgag aaatcctctg ggcgatgtcc
     1921 cgaacatggc gccgaacgcg gccatggatg cctggatctc gccgccgtag ccgcgcgcat
     1981 tctgctcggg aaacacaccg cccagcggca gggacccgaa tcggtaatcg gattggccat
     2041 cgggcactcc gttgttcag
//
LOCUS       DBNGSH010000672         4259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2448127, whole genome shotgun sequence.
ACCESSION   DBNGSH010000672 DBNGSH010000000
VERSION     DBNGSH010000672.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2448127"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="AC16CY_15525"
     CDS             complement(<1..1520)
                     /locus_tag="AC16CY_15525"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355258.1"
                     /translation="MPISTGTRLGPYEVTGTLGVGGMGEVYRATDTSLGRSVALKVLP
                     EAFAHDADRLARFEREARTLAALNHPNIAAVYGLEKSSGVHALVMELVDGPTLADRIV
                     HGAIPLDEALAIAKQIAEALEAAHEQGIVHRDLKPANIKVRDDGAVKVLDFGLAKQAS
                     GPGPQASDRGPDGILSQSPTITTPAMTMAGMILGTAAYMSPEQAKGRPADKRSDIWAF
                     GCVLYETLTGRRAFEGDDVTDTLAFVITKEPDWTRLPPKTPATVRRLLRRMLTKDRRQ
                     RLSDVAAIRLDVEETLAAPVGVATDAALATTWRRTASIAATAALVVGLAAAAAAWWAT
                     RPDPPRMTRLSISAAAMTLSSNNRDIAITRDGSRVVYLGNNGTQLVVRAMDQLEPTPL
                     TGLGSPTAPFFSPDGQSIGFVDLGFDLKKVRITGGPAVRICRLDGAGRGASWGPDGTI
                     VFATSTTLTGLQQVSAAGGEPTVLTKPDAARGEGDHYWPEFLPGGRAVLFTVVATTG"
     gene            complement(1575..>4259)
                     /locus_tag="AC16CY_15530"
     CDS             complement(1575..>4259)
                     /locus_tag="AC16CY_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355259.1"
                     /translation="RMPLATGARLGPYEVTGTLGAGGMGEVYRATDTSLGRSVALKVL
                     PEAVAHDADRLARFEREARTLAALNHPNIAAVYGLEKSSGVHALVMELVDGPTLADRV
                     VHGAIPLDEALAIAKQIAEALEAAHEQGIVHRDLKPANVKVRPDGTVKVLDFGLAKAS
                     DPRGSSPVPPSSAVATAMTDTGIILGTAPYMSPEQACGKAVDKRTDIWAFGCVLYEML
                     TGRATFSGDSTPATLIAIVEREPEWAALPPATPASVRRLLERCLVKDPKLRLRDVGDA
                     RADLIEGPPSPPSQSPPRASRLRAAVQWVLVPALTAAAAALLVWYVEPASPSPGSSAG
                     AIARLVITAPPGEPLATDTVGVAISPDGRRVAYVAGRGNRRIYLRDIDQFDSIPLPGT
                     EGANSAFFSPDGESIGFVAGGRMKRVRVAGGSPQTITETVQTASAFTASWESDDTIFF
                     TPVPGTGIWRVPATTGGSPTALTKLTETESTHRWPQLLPGGKALLFSAAGTSGSQSYV
                     QLLETGARRPLVKGEGTRYLPTGHLVYTQGGTLVAVPFDPERLEITGPPFTAFSGVLQ
                     MNRLRNSTVTNLVPQIAFSNAGSVAYVPASRRPPQHALVWVNRTGVEQPTGAYGGVYY
                     QPRISPDGRRVAVTIGGEDFDDVWVYDLTRHSWSRFTTTGGNNAFPLWAPEGDRLTYV
                     SDRAGPDNMYSKPLDDSGPERRLAVSDTANYPFGWSKDGTLVFVATDPGTLQDIWVLR
                     PGEGAKPEPLVRTRYGEGAPAISPDGRWLAYASNESGRNEVYVRPFQGPGERSTVSID
                     GGNEPVWSPTGRELFFRNGDAMMAVDVSAGPSFAASKPRLLFEKHYQASQALWPNYAV
                     APDGRFVMVKALDQEGGPTQINVVVNWFDELKRLATPK"
BASE COUNT          677 a         1442 c         1410 g          730 t
ORIGIN      
        1 cctgtcgttg caacgacggt gaagagcacg gcacgtccac ccggtaggaa ttccggccaa
       61 tagtggtcgc cctcgccgcg agcggcgtcc ggcttggtca ggactgtcgg ttcccctccg
      121 gccgccgaca cctgttgaag tccggtcaga gtcgtgctgg tggcgaagac aatggtcccg
      181 tcggggcccc acgaggcgcc acgccccgct ccgtcgaggc ggcagatccg cacggctggt
      241 ccgccggtaa tcctgacctt cttgagatcg aagccgaggt cgacgaagcc aatcgactgt
      301 ccgtcgggcg agaagaacgg cgccgtcggc gatccgaggc cggtcagcgg agtcggttcg
      361 agctggtcca tcgcgcgcac gacaagttgt gtgccattgt tgccgaggta gacgacacgt
      421 gagccatccc gggtgatcgc gatatcacgg ttgttgctgc tgagcgtcat tgccgccgcc
      481 gatatggaca atcgcgtcat acgcggcggg tccgggcgcg ttgcccacca cgcggccgcg
      541 gcggcagcga ggccgacgac gagcgccgct gtcgcggcga tcgatgccgt acgccgccac
      601 gtcgtcgcca gtgcggcgtc tgtcgccacc cccacgggcg ctgccagcgt ctcctctacg
      661 tccagtcgta tcgccgcgac atccgacagc cgctgccgac gatctttagt gagcatccgc
      721 cgcaacaggc gacgaaccgt ggccggagtc tttggaggca gtcgcgtcca gtcgggctcc
      781 ttcgtgatca cgaaggccag cgtgtcggtg acgtcgtccc cctcgaacgc gcgccgcccg
      841 gtgagcgtct cgtagagcac gcagccgaac gcccagatgt cgctgcgctt gtcggccggc
      901 cggcccttgg cttgctccgg cgacatgtac gccgccgttc ccagaatcat cccggccatc
      961 gtcatggctg gagtcgtgat ggtgggtgac tgcgacaaga tcccgtcagg gcctcgatct
     1021 gaggcctggg gcccggggcc tgaggcctgc ttggccagac cgaagtccag caccttcacg
     1081 gcgccgtcgt cgcggacctt gatgttcgcg ggcttcagat cgcgatggac gatcccctgt
     1141 tcgtgcgcgg cctcgagcgc ctccgcgatc tgcttggcaa tcgccagcgc ttcgtccaac
     1201 gggatcgccc catggacgat gcggtctgcg agcgtcgggc cgtcgaccag ttccatgacg
     1261 agcgcgtgca cgccgctcga tttctccagc ccgtacacag ccgcgatgtt cgggtgattg
     1321 agcgcggcca gcgtccgcgc ttcacgctcg aagcgtgcga gccgatcggc gtcgtgcgcg
     1381 aacgcctccg gcagtacctt gagggcgacg ctgcgcccca ggctcgtatc ggtggcgcga
     1441 tacacctcgc ccatgccacc gacacccagc gtgccggtga cctcgtacgg accgaggcgc
     1501 gtgcctgtcg aaatgggcat tcgcggaccg acgagattct gtcacaaggg aatgaggacg
     1561 cgggcggtgc tctcctattt cggcgtcgcg aggcgcttca actcgtcgaa ccagttgacc
     1621 acgacgttga tttgggtcgg gcctccctcc tgatcgagtg ccttgaccat gacgaacctg
     1681 ccatccggtg ccacggcgta gttgggccag agcgcttgag acgcctggta gtgtttctcg
     1741 aacagcagtc gcggcttgct ggccgcgaag gagggccccg ccgacacgtc gacggccatc
     1801 atcgcgtcgc cgttgcgaaa gaacaactcg cgacccgtcg gggaccagac tggctcgttg
     1861 ccgccgtcga tcgaaaccgt agaccgttcg cccggccctt gaaacggccg gacgtagact
     1921 tcgttgcgcc ccgattcatt cgacgcgtac gcgagccagc ggccgtccgg tgagattgcc
     1981 ggtgcccctt cgccgtaccg cgtgcgaacc aacggctccg gcttggctcc ctcacccggt
     2041 cggagaaccc agatatcctg cagagtgcca ggatcggtcg cgacgaatac cagagtgcca
     2101 tccttcgacc agccgaaggg gtagttggcc gtgtcgctca ccgcgagtcg ccgttcgggc
     2161 ccgctgtcat ccagaggctt cgagtacatg ttgtccggac ccgctctgtc ggatacatat
     2221 gtcagccgat ccccttcggg cgcccagagc ggaaacgcgt tgttccctcc cgtcgtcgtg
     2281 aaacggctcc atgaatgtcg cgtaaggtcg tacacccaga catcatcgaa atcctcgccg
     2341 ccgatcgtga ctgcgacgcg cctgccgtcc ggggaaatgc gcggctggta gtagacgccg
     2401 ccgtacgcgc cggtgggttg ctcgacgccc gtgcggttca cccacacgag ggcgtgctgc
     2461 ggcggacgcc gactggccgg cacgtacgca acactccctg cgttcgaaaa ggcgatctgc
     2521 ggcacgaggt tggtcactgt gctgttgcga agccgattca tctgcaagac gccggagaaa
     2581 gccgtgaacg gaggtcccgt gatctccagc cgttccggat cgaagggcac ggctacgagc
     2641 gtgcccccct gggtgtagac aagatgccct gtcggcagat agcgggtccc ttcgcccttg
     2701 accagagggc gccgcgcacc ggtttcgagc agctgcacgt aggactgcga gccggaagta
     2761 ccggccgcac tgaacaggag ggccttccct ccgggcagga gctggggcca ccggtgagtg
     2821 ctttccgtct ccgtcagttt cgtcagtgct gtcggcgagc cgcctgttgt cgccgggacg
     2881 cgccagatgc cggtccccgg caccggcgtg aagaagatcg tgtcatcgct ctcccagctc
     2941 gcggtgaagg cgctcgccgt ctgcaccgtc tcggtgatgg tctgtgggga cccgccggcg
     3001 acccgtaccc tcttcattct tccgccggcg acgaaaccga tcgactcccc gtccggtgaa
     3061 aagaacgcgc tgttggcgcc ctcggtgccc ggcagcggga tgctgtcgaa ctgatcgatg
     3121 tcacgcaggt agattcgccg gttgccgcgg ccggccacgt aggcgacacg gcggccatcc
     3181 ggtgagatgg ccacgccgac cgtgtcggtc gccagcggct caccaggcgg agccgtgatc
     3241 acgagacgcg caatcgcgcc tgccgaagat cccggggacg gcgaggcagg ctcgacgtac
     3301 cacaccagca gcgcagccgc cgccgcggtg agagccggga cgaggaccca ttgaacggcc
     3361 gcccgcaggc gggatgcgcg cggtggcgat tggctcggcg gcgagggggg gccttcgatc
     3421 agatcggcac gcgcatcgcc cacgtctcgg agccgtaact tcggatcctt taccagacaa
     3481 cgctcgagga ggcgccgcac ggaggcaggc gtcgccggag gcagggcggc ccactcgggc
     3541 tcacgctcca cgatcgcgat aagggtcgcc ggtgtcgagt ctcccgaaaa cgtcgcccgg
     3601 ccggtcagca tctcgtaaag cacgcacccg aacgcccaga tatccgtgcg tttgtcgacg
     3661 gcttttccac acgcctgttc aggactcatg tatggcgcgg taccgaggat gattcccgtg
     3721 tcggtcatcg ccgttgcgac ggccgacgat ggcggaacgg gactgctgcc gcgcggatcg
     3781 gaggccttcg ccaggccgaa atcgagcacc ttgaccgtac cgtctggacg caccttgacg
     3841 ttggcgggct tcagatcgcg atggacgatc ccctgttcgt gcgcggcctc gagcgcctcc
     3901 gcgatctgct tggcaatcgc cagcgcttcg tccaacggga tcgccccatg gacgacgcgg
     3961 tctgcgagcg tcgggccgtc gaccagttcc atgacgagcg cgtgcacgcc gctcgatttc
     4021 tcgaggccgt agaccgcggc gatgttcggg tgattcagcg cggccagcgt gcgcgcttca
     4081 cgctcgaagc gtgcgagccg atcggcgtcg tgcgcgactg cctccggcag taccttgagg
     4141 gcgacgctgc gccccaggct cgtatcggtg gcgcgataca cctcgcccat gccgccggca
     4201 cccagcgtgc cggtgacctc gtacggaccg agtctcgcgc ctgtcgccag gggcattcg
//
LOCUS       DBNGSH010000673         8889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1599389, whole genome shotgun sequence.
ACCESSION   DBNGSH010000673 DBNGSH010000000
VERSION     DBNGSH010000673.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1599389"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="AC16CY_15535"
     CDS             <1..771
                     /locus_tag="AC16CY_15535"
                     /inference="COORDINATES: protein motif:HMM:NF012253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3355260.1"
                     /translation="DVSTLPTKGNGAMAVLPSEDSLTALDVGRMLLEAGANPNLQLKR
                     RPPYRDVPQDRGGDTILAQGATPLLRASRAGDAPFVELLLKHKALVDLPSKEGVTPLM
                     AAAGVEFGDRVTRGRNRTNEGVLATMRLLLDAGADVNARMVTEPGRNTADGASQAAAF
                     ATGLRRAGQMPSAMAVPHQTALHGAAQRGFTPFVKFLAENGADLQAKDANGRTALDLA
                     KGVGVAGVRQASREPFPETVALLESLMASTTIPRSPSQ"
     gene            775..2109
                     /locus_tag="AC16CY_15540"
     CDS             775..2109
                     /locus_tag="AC16CY_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR3355261.1"
                     /translation="MRGDDLLARDLASLVHPLHDAKAHRAGRVWVRGHGALLVDADGN
                     ELIDGLAGLWNVSLGHGRRELAEAAREQLETLAYASGYCGSSNLRAIELAERLARITY
                     PAINRFFFTSGGAEATESAIKMARSYWKLRGRPAKTKVISRDLAYHGTTLAAMSATGI
                     AGYWPLFEPRVPGFIHVPSPYPYRATATPGSTYGIAVANELEKAILREGADTVAMFLA
                     EPVMGVGGVIVPPDDYFPRIREICDAHDVLFAADEVITGFGRTGRWFALEHWGIAPDI
                     VQFAKGITSGYFPLGGIGISDAIARVLDDSPSPWMHAHTYSGHPVGCALALRTLRIIE
                     EENLVAEAARMGAYLMGALRAHLGGHPHVGEIRGKGLMCAVEFVRDRDTRQPFAPDDQ
                     VGARIHTEAMARGLVSRVRGDVFVLAPPFVTPDDLLDRIAGILASAAKAVLA"
     gene            complement(2116..2589)
                     /locus_tag="AC16CY_15545"
     CDS             complement(2116..2589)
                     /locus_tag="AC16CY_15545"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3355262.1"
                     /translation="MRIVAGGLDDPRVQALLEQHARTARAQTAAGSAHALDLGGLQSA
                     DMTFWSAWDGDRLVGAGALKRLSPTHGEIKSMHTVEASRRTGVGTALLRHIVSAARTM
                     GMKRLSLETGSWPYFQPARAFYRTHGFVECPPFADYREDPNSIFMTRTLEDPALD"
     gene            complement(2586..3692)
                     /locus_tag="AC16CY_15550"
     CDS             complement(2586..3692)
                     /locus_tag="AC16CY_15550"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HIR3355263.1"
                     /translation="MHRDTRILQAGYAPVELAGPLVDGPQFSATYTTPGQPSDHQWLY
                     GRFQNPTWTVWEAALAELEGGAAVAFASGMAAIAAVFGVCLKPRDIVVLPSDSYYTTR
                     VLASSWLASMGVDVRLAPTRGNQQAGALEGARLVWLETPSNPQLDICDIRLLVDAAHA
                     RGALVAVDNTTATGYLQQPLALGADYVVASDTKAMTGHSDLVLGHVAATTPEAIDAIR
                     RWRTEHGAIPGPMETWLAHRSVATLPLRLRQQCASALSVATLLASKPAVRTVRYPGLP
                     GHPGHEIATRQMHAFGCVVSFDLASRERADAFLTGSKLIRQATSFGGVHSTAERRARW
                     GGDAVSEGFVRLSVGCESIDDLLEDIESALDRAT"
     gene            complement(3695..3997)
                     /locus_tag="AC16CY_15555"
     CDS             complement(3695..3997)
                     /locus_tag="AC16CY_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3355264.1"
                     /translation="MISPQIVKALANERRLDIMNWLKAPRRHFPAQVDGDLVKDGVCG
                     LFIANKLGVGQPTASEHLRILTQAGLIRGKRIKKWTFYKRNEAVINHVKRAFKTAW"
     gene            4044..5027
                     /locus_tag="AC16CY_15560"
     CDS             4044..5027
                     /locus_tag="AC16CY_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HIR3355265.1"
                     /translation="MEETELGVIDRPAIQRTYAVIRPYIRQTPVVEVDGRDFGLGPCS
                     LAFKLEQLQRAGSFKTRGAFAHLLTRSIPAAGVAAASGGNHGAAVAYAARDMGVPAHI
                     FVPRVSSRAKVDRIRSYGATLTIAGERYEDALSASEGWAAEARARTIHAFDQAQTLLG
                     QGTVGLEFAAQLPTLDTLLVAVGGGGLIGGIAAWFAGTVRVVGVEPLSAPTLAAALKA
                     GRPVDVPAGGVAADSLASRRVGELAFPIARRYVDRVVLVRDADIVRAQHSLWDVARIV
                     AEPGGATALAALLSGEYAPEPGEKVGVVISGANTTVVDFGAQVSGADLTIR"
     gene            5108..7069
                     /locus_tag="AC16CY_15565"
     CDS             5108..7069
                     /locus_tag="AC16CY_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3355266.1"
                     /translation="MTRRLVHLLAVLTLFDVCTGARAQQTAQRRAPGFDVEEATIERI
                     QQAILKKQITTQGVVEAYLKRIHAYNGTCVNEPQGILGPITPIPRAGQINALSTLNLR
                     PRTRAAWGFDARKARSLTDVVDEARSMPDALETAAAQDQQFAKTGRLVGPLHGVVLAI
                     KDQYDTFDMRTTSGADADYANDRPPDDATFVRRLRDAGAIILAKANLAEYAVDGARSS
                     FGGTFCNPYDTEREPGMSSAGSATAVAANLVTCAIGEETVVSIRWPAAVNSLVGLAPT
                     QELVSRDGMMGAGLNMRVGPMCRTVQDAARVLDAFAGYDPKDEMTVYSVNRKPSQPYA
                     SFAAEKRLDGVRIGVLREYMSRKLFAQADEESIGLVERAIADLRRLGATMVDPGAAGE
                     LFTRCIARYAPELANSAFARQYPRLFPQDAAPPGGDQIETLLQLRQDPTRVPPELSLR
                     TLNAGGFGATGEGKYWINRYLRERGDANIRSNADLITKARFYQDPNFPDRKQAREQAE
                     RATALDTSVRLQGRFAVQTVLLQCMQEQRLDALVSPMSTIPPRKLTAPREPAVNGRTP
                     IGWSLIGQQGFPVITVPAGFARSIWDRVAEGNGTRLVGPVSAALPVGVDFIARPFDEP
                     LLFRIASAYEAATRHRRPPPDFGPLPARR"
     gene            7151..8608
                     /locus_tag="AC16CY_15570"
     CDS             7151..8608
                     /locus_tag="AC16CY_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3355267.1"
                     /translation="MRRLLFLLIAIAVAAGCARKRPPVVEPAPPVPLVDVASLIQRGC
                     YRCLEDAYAAAVKQGQTNLAFEAALLLVLRSKELGLPPAQWLERANAATENDPLRIEY
                     ERMVAAVPVDPLSGDREAIFGRPREFIDPQTPSARLKTVAGGSASRELRAYLEGALVC
                     VFSTAEDIDAFVKSIEETAAPLLQYRAGLCRAEHASRLRRLQESEGGFVDADYTLGRY
                     ARDDAAPDQEEAMRRFQSASAAFPASTSIPTAIGNLYRAWEEWALASTAYESALALRP
                     THPDALLGRTIALAHLSRHQESLETATRLIEVGRWFQGEAYYWRGWNYLQLKDYPAAR
                     TATDLAKSLMVNASVFVLSGMIEWSMMRLDTAEREFEQALTMDFGQCDAALFLGGVRA
                     EMPKLEPALAALNQARQCYDLSITVRRKLIEGILAAKGSEQTKARQVASQERAIAISE
                     SRRADATRAAAQVQAHLDARKPPPPPPTPSRAPRR"
     gene            8817..>8889
                     /locus_tag="AC16CY_15575"
     CDS             8817..>8889
                     /locus_tag="AC16CY_15575"
                     /inference="COORDINATES: protein motif:HMM:NF019324.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HIR3355268.1"
                     /translation="MALNIKNTEVERLATEVARLTGES"
BASE COUNT         1431 a         2993 c         3048 g         1417 t
ORIGIN      
        1 gacgtcagca cgctcccgac caaggggaac ggggcgatgg ccgtgctccc gagcgaggac
       61 tcgctgaccg cgctcgacgt cggcaggatg ctgctcgagg ccggcgccaa tccgaacctt
      121 cagctcaagc gtcgcccgcc gtaccgcgac gtgccgcagg accggggcgg cgacacgatt
      181 cttgcgcagg gggccacgcc gctgctgcgc gcctcgcgcg ccggcgacgc gccgttcgtc
      241 gagctgctgc tgaagcacaa ggcccttgtc gatctgccga gcaaggaagg cgtgacgccg
      301 ctgatggcgg cggccggcgt cgaattcggc gaccgcgtga cgcgcgggcg caatcgcacg
      361 aacgaaggcg tgctcgccac catgcggctg ctgctcgacg cgggtgcgga cgtcaacgcc
      421 aggatggtga ccgagccggg gcgcaacacg gccgacggcg cctcgcaggc agcggcgttc
      481 gccaccggcc tccgccgtgc gggtcagatg ccgagcgcga tggccgtgcc gcatcagacg
      541 gcgctgcacg gcgcggcgca gcgcgggttc acgccgttcg tgaagttcct cgccgagaac
      601 ggggccgacc tgcaggcgaa ggacgccaac ggtcgtaccg cgctcgacct cgcaaagggc
      661 gtcggcgtcg cgggcgtccg ccaggcctcg agggagccgt tcccggagac ggtcgctctg
      721 ctcgaatcgt tgatggcttc cacgaccatc ccgcggtcgc cttcgcaata gccggtgaga
      781 ggcgacgacc ttctcgccag ggatctggcg tccctcgttc atccccttca cgatgcgaag
      841 gctcatcgag ccggacgcgt gtgggtgcgc ggccacggcg cgctgctggt agacgccgac
      901 gggaacgagc tcatcgacgg ccttgccggg ctgtggaacg tctcgctcgg ccatggccgc
      961 cgggagctgg ccgaggccgc ccgcgagcag ctcgagacgc tggcctatgc ctcgggctac
     1021 tgcggaagct cgaacctgcg cgccatcgag ctggccgagc gtcttgcccg tatcacgtac
     1081 ccagccatca accggttctt cttcacctcg ggcggcgccg aagccaccga gagcgcgatc
     1141 aagatggcgc gctcgtactg gaagcttcgg ggccggccgg ccaagacgaa agtcatttcc
     1201 cgcgacctcg cgtaccacgg cacgacgctg gcggcgatga gcgccactgg gatcgccggc
     1261 tactggccgc tcttcgagcc gcgggtcccg ggcttcatcc acgttccgtc gccgtatccg
     1321 tatcgcgcaa cggccacccc gggatcgaca tacggcatcg cggtggccaa cgagctggag
     1381 aaggccatcc ttcgcgaggg cgcggacacg gtggcgatgt tcctcgccga gcccgtgatg
     1441 ggcgtcggcg gcgtcatcgt tccgcccgac gactacttcc cgcgcatccg cgagatctgc
     1501 gacgcgcacg acgtgctctt cgccgccgac gaggtgatca ccgggttcgg ccgcaccggc
     1561 cggtggttcg cgctcgaaca ctggggcatc gcccccgaca tcgtgcagtt tgccaagggg
     1621 atcacgtcgg gctatttccc gcttgggggc atcggcatca gcgacgcgat tgcgcgcgtg
     1681 ctggatgact cgccatcccc gtggatgcac gcccacacct acagcggtca tccggtggga
     1741 tgcgcgctgg cgctgcgcac gctccggatc atcgaggaag agaacctcgt cgccgaggcg
     1801 gcgcgcatgg gcgcatatct gatgggggct ctgcgcgcgc atctcggggg ccatccgcac
     1861 gtcggggaga ttcgcggcaa aggactcatg tgcgccgtgg agttcgtcag ggaccgcgac
     1921 accaggcagc cgtttgcgcc cgacgatcag gttggcgcgc gcattcacac ggaggccatg
     1981 gcgcgtggcc tggtcagtcg tgttcgaggc gacgtcttcg tcctggcgcc gccgttcgtc
     2041 acgcctgacg atctgctcga ccgcattgcc gggatcctgg cgtcggccgc caaggccgtg
     2101 ctcgcatagc ccccgtcagt cgagggcagg atcttccagc gtgcgcgtca tgaagatgct
     2161 gtttggatct tcccgatagt cggcgaatgg agggcattcg acgaagccgt gcgtgcgata
     2221 gaaggcacgt gccggctgga aatacggcca cgaaccggtc tcgagactca accgcttcat
     2281 gcccatggtt cgtgccgcgc tgacgatgtg gcggagcaga gccgtcccga ctccggtccg
     2341 ccggctggcc tcgaccgtgt gcattgactt gatctcgccg tgcgtcggcg acagccgttt
     2401 cagcgccccg gcgcccacca gtcgatcgcc atcccacgcc gaccaaaacg tcatgtcggc
     2461 cgactgcaag ccgccgaggt cgagcgcatg agcgctgccg gccgcggttt gggcgcgggc
     2521 cgttcttgcg tgctgttcca gcaacgcctg gactcgcgga tcgtcgaggc cgccggcgac
     2581 aatcctcatg tggcgcgatc gagcgcagat tcgatgtctt cgagcagatc gtcgatggac
     2641 tcgcagccga cgctcaaccg cacgaatccc tcgctgacag catcgccgcc ccagcgcgcg
     2701 cgccgttccg cggtggaatg cacgccgcca aagctcgtcg cttgccgaat cagtttcgag
     2761 ccggtcagaa aggcatcggc gcgctcgcgt gaggccagat caaagctcac gacgcacccg
     2821 aaggcgtgca tttgccgtgt ggcgatctcg tggcccggat gcccgggaag gcccgggtac
     2881 cgcacggtcc gcacggcggg cttcgacgcc agaagcgtgg cgaccgaaag ggcggaggca
     2941 cattgttgcc gcaggcgcaa cggcagcgtc gcaaccgaac gatgggctaa ccatgtctcc
     3001 atcggaccgg gaatcgcgcc gtgctctgtc cgccagcggc ggatcgcgtc gatcgcttca
     3061 ggagtcgtcg cggccacgtg accgagcacc agatcgctgt gcccggtcat ggccttcgtg
     3121 tcggatgcga cgacgtagtc cgcgccgagc gcaagaggct gctgcaagta cccggtcgcg
     3181 gtcgtgttgt ccaccgccac cagggcccct cgcgcgtgcg ccgcatccac gagcaggcga
     3241 atatcgcaga tatcgagctg cgggttcgaa ggggtctcca gccacacgag ccgcgcgccc
     3301 tcgagcgcgc ccgcctgctg gttcccgcgc gtgggtgcga ggcgcacgtc cacgcccatt
     3361 gacgcgagcc acgatgacgc cagcacgcgc gtcgtgtaat acgaatcaga cggaaggacg
     3421 acgatgtcgc gcggtttcag gcacaccccg aagacggcgg caatggcggc catccccgag
     3481 gcaaacgcga cggccgcgcc gccttccagt tcggcgagcg cggcttccca gaccgtccac
     3541 gtcggattct gaaaccgacc gtatagccac tgatgatctg atggctgccc gggtgtcgtg
     3601 taggtggccg agaactgcgg gccatcgacg agaggaccgg cgagctcgac gggtgcgtag
     3661 ccggcttgca gaatccgggt gtctcgatgc atggctacca ggccgtcttg aaggcgcgtt
     3721 tgacgtggtt gatgaccgct tcgttgcgct tgtagaacgt ccacttcttg atccgtttcc
     3781 ctcgaatcaa tccggcctgc gtcaggatcc ggagatgctc gctcgcggtt ggctggccga
     3841 cgccaagctt gttggcgatg aagaggccgc acacgccgtc tttgacgaga tcgccatcga
     3901 cctgcgccgg aaagtgccgt ctcggcgcct tgagccagtt catgatgtcc aggcgccgct
     3961 cgttggccag tgccttcacg atctgcggtg agatcatgtc gacactttgc cattcgacga
     4021 agtgtgtgtc aagatgcgcg gccatggagg agacagagct cggcgtcatc gaccgtccgg
     4081 cgatccaacg gacctacgcc gtcatcaggc cgtacattcg ccagacgcct gtcgtcgagg
     4141 tcgatggacg cgacttcggg ctcggcccgt gctcgttggc gttcaagctc gagcagcttc
     4201 agcgtgccgg ctccttcaag acccgtggag cgttcgcgca ccttctcacc cgatcgatcc
     4261 cggccgccgg ggtggcggcc gcgtcaggcg gcaatcacgg cgccgcggtt gcctacgccg
     4321 cgagggacat gggggtgccg gcgcacatct tcgtccctcg cgtgtcgtcg cgggccaagg
     4381 tcgatcggat ccgttcgtac ggcgcgacgc tgacgatcgc cggtgagcgc tatgaggatg
     4441 cgctgtccgc gagcgagggc tgggccgctg aggcgcgcgc gcgaacgatt catgcgttcg
     4501 accaggccca gacgctgctg gggcagggta cggtcggcct cgagttcgcg gcgcagttgc
     4561 cgacgctgga cacgctcctc gtcgcggtgg gcggcggcgg cctgatcggg ggaatcgcgg
     4621 cctggttcgc ggggactgtg cgcgtcgtgg gcgttgagcc gctgagcgcg ccgacgctgg
     4681 ctgcggcgct gaaggccgga cgtcccgtgg acgtaccggc aggaggtgtg gccgcggatt
     4741 cgctggcttc gcggcgagtc ggtgaactgg cgtttccgat cgcccggcgc tacgtggatc
     4801 gagtcgtcct ggtgcgcgac gcagacatcg tgcgcgcaca gcactcgctc tgggacgtcg
     4861 cgcgaatcgt tgcggagcct ggaggggcga cggcgttggc cgcgctcctg tccggtgagt
     4921 acgcgccgga accaggagag aaagtgggcg tcgtcatcag cggcgcaaat accacggtag
     4981 tcgactttgg tgcgcaggtg tcgggcgcgg atctgacgat tcggtgagct cccggtcggc
     5041 gccttgactg cgtgcggtgc ccccggatgt ggcagactat cccgccagtg tccccgtggg
     5101 taggcgcatg acacgccgcc tcgttcatct actagccgtc ctgacgctgt ttgacgtctg
     5161 cacgggtgcc cgtgcccagc agacggcgca gcggcgcgcg ccgggcttcg acgtggagga
     5221 agccacgatc gaacggatcc agcaggcgat cctcaagaaa cagatcacga cgcaaggcgt
     5281 cgtcgaagcc tacctgaagc gcattcacgc gtacaacggc acgtgcgtca acgagccgca
     5341 ggggattctg gggccgatca cgccgatccc gcgcgccggg cagatcaacg cgctgtccac
     5401 gttgaacctg cgcccgcgca ctcgcgccgc atggggcttc gacgcgcgga aggcgcgcag
     5461 cttgaccgac gtggtggacg aggccaggag catgccggac gcgctcgaga ccgccgccgc
     5521 ccaggatcag cagttcgcga agaccggccg cctcgtcggg ccgctccacg gggtcgtcct
     5581 cgcgatcaag gaccagtacg acaccttcga catgcgcacg acgtccggcg ccgacgccga
     5641 ctacgccaac gaccgccctc ccgacgatgc gacgttcgtc aggcgattgc gggacgcggg
     5701 cgccatcatc ctggccaagg ccaacctcgc cgagtacgcc gttgacggcg cccgaagctc
     5761 gttcggcggg accttctgca acccgtacga caccgagcgc gagccgggga tgtcgagcgc
     5821 cggctcggcc acggcggtcg ctgccaacct cgtcacctgc gccatcggcg aggagaccgt
     5881 cgtctcgatc cgctggccgg ctgccgtaaa cagcctcgtc ggactggcgc cgacacagga
     5941 gctggtcagc cgcgacggaa tgatgggcgc cgggctcaac atgcgcgtcg gtccgatgtg
     6001 ccgaacggtg caggacgcgg cgcgcgtgct cgacgccttc gccggctacg acccgaagga
     6061 cgagatgacg gtgtacagcg tgaaccgcaa gccgtcacag ccctatgcca gtttcgcggc
     6121 cgagaagcgc ctcgacggcg tgcgcatcgg cgtcctgcgc gagtacatga gccggaagtt
     6181 gtttgcgcag gcggacgagg agagcatcgg cctggtcgag cgcgcgatcg ccgatctgcg
     6241 caggctcggc gcgacgatgg tcgaccccgg cgcggcgggc gagctgttca cccgctgcat
     6301 cgcccgctac gcgccggagc tcgccaactc ggcgttcgcc cggcagtacc cgcggttgtt
     6361 tccacaggac gcggcgccgc cggggggtga tcagatcgag acgctgctgc agctccgcca
     6421 ggatcccacg cgcgtaccgc cggagctgtc gctgcgaacc ctgaacgcgg gaggcttcgg
     6481 cgcgacggga gaaggaaagt actggatcaa ccggtacctg cgcgagcgcg gcgacgcgaa
     6541 catcaggagc aacgcggacc tcattaccaa ggcgcggttc tatcaggacc cgaacttccc
     6601 ggatcgcaag caggcgcgcg agcaggccga acgcgccacg gcgctggaca cgtcagtccg
     6661 gctgcagggg cggttcgccg tgcagacggt gctgctgcag tgcatgcagg agcagcgcct
     6721 cgacgcgctc gtgtcgccga tgtcgacgat tcctccgcgc aagctcaccg ctccgcgcga
     6781 gcccgcggtc aacggccgca cgccgatcgg ttggtcattg atcggtcagc agggatttcc
     6841 ggtcatcacg gtgccggccg gatttgcgag atcaatctgg gatcgcgtgg ccgaaggcaa
     6901 cgggacgcgc ctggtcggtc cggtgtcggc cgcgctcccc gtcggtgtgg acttcatcgc
     6961 gcgtcccttc gacgagccgc tgctcttcag gatcgcgtcg gcctacgaag cggcgaccag
     7021 gcatcgacgg ccgccgcccg atttcgggcc gctgccggcc aggcggtgaa gtcgtcaacg
     7081 cccaacgccc aacgcccaac tcccaaagcc caagcgggtc cgtttcgccg tttttggtgt
     7141 ctaaccggcc atgcggcgtc tgctattcct tctcatcgcg atcgccgttg cggcgggttg
     7201 cgccagaaaa cgtccgcctg tcgtcgagcc ggcgccgcca gttccgctgg tcgatgtcgc
     7261 ctcgctgatc cagcgtggct gctaccgctg tcttgaagac gcgtatgccg cggcggtgaa
     7321 gcagggccag acgaacctgg ccttcgaggc cgcccttctg ctggtcctgc gatcgaagga
     7381 actgggcctc ccccccgcgc agtggctgga gcgcgcgaac gcggccacgg agaacgatcc
     7441 gctgcggatc gaatacgaga ggatggtcgc cgccgtacct gtggacccct tgagcgggga
     7501 tcgcgaggcg atctttggca ggccgaggga attcatcgac cctcagacgc ccagcgcgag
     7561 gctgaagacg gttgccggcg gcagcgcctc gcgcgagttg cgcgcgtacc tcgaaggcgc
     7621 gctcgtgtgc gtcttctcca cggcggagga cattgacgcg ttcgtgaagt ccatcgaaga
     7681 gacagccgcg ccgctgctgc agtaccgggc cggcctctgc cgcgccgagc atgcctcgcg
     7741 cctccgccgg cttcaggagt ccgagggcgg gttcgtggac gccgactaca cgctcggccg
     7801 ctacgcgcgc gacgatgccg ctcccgatca ggaagaagcg atgcggcggt ttcagtccgc
     7861 ctctgcggcg tttcccgctt caacctcgat ccccacggcg attggcaacc tctatcgagc
     7921 atgggaggaa tgggcgctgg ccagcaccgc gtacgaatcg gccctcgcgc tgcgtcccac
     7981 ccatcccgac gcgctgcttg gccggacgat cgccctcgcg cacctcagcc gccaccagga
     8041 gagcctcgag acggcgacac gcctgatcga ggtcgggcga tggtttcagg gcgaggcgta
     8101 ctactggcgg gggtggaatt accttcagct gaaggactac ccggctgccc ggacagcgac
     8161 cgacctcgcg aagtcgttga tggtcaacgc gtcggtcttc gtcctgtccg gcatgatcga
     8221 gtggtcgatg atgcgtctgg ataccgccga gcgcgaattc gagcaggcgc tgacgatgga
     8281 cttcgggcag tgcgacgcgg cgctgtttct cggcggcgtg cgcgccgaga tgccgaagct
     8341 cgagccggcg ctggccgcgc tcaaccaggc gcggcagtgc tacgacctct cgattacggt
     8401 caggcgaaag ctcatcgagg gcatcctggc agcgaaaggg agcgagcaga ccaaggcgcg
     8461 gcaggtggca tcccaggagc gggcgatcgc gatcagcgag tcgcgcaggg ctgatgccac
     8521 gcgcgcggcg gcgcaggtcc aggctcatct cgacgcccgg aagccaccgc cgcccccgcc
     8581 aacgccgtcg cgcgcgccac gacggtaacg tcgtcacttg agcaagagat ctgcaagaga
     8641 aacgactggt ctctgctcga ttcacctgac ctctgggatc agcgtgaccg agcgcggcct
     8701 gcggggctga gctcccgaag ccgccaggcc ttcgccgtct tccagcaatc acggatcatc
     8761 aatcgacgtc cacgtttaca cccttacacc acgagatcgg ctaccatggt gtagtcatgg
     8821 ccctgaacat caagaacacc gaggtcgagc ggttggcgac ggaagttgcg cggctgacgg
     8881 gtgagtcga
//
LOCUS       DBNGSH010000674         1849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_240756, whole genome shotgun sequence.
ACCESSION   DBNGSH010000674 DBNGSH010000000
VERSION     DBNGSH010000674.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_240756"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="AC16CY_15580"
     CDS             complement(<1..1121)
                     /locus_tag="AC16CY_15580"
                     /inference="COORDINATES: protein motif:HMM:NF013179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3355269.1"
                     /translation="MKIGFRTKVFVGSFAAAGVSLLALAVLVSWQVRERQRAAIEQRL
                     TDQAHLIAELLTRATSIGEDQLDEEADRLGELVPGRVTFIARDGRVLGDSTQSPAALL
                     EIDNHASRPEVAAARENGVGSSHRYSTTVGVGMVYVAVRASHPLVGYVRLALPLTDID
                     AQLATIRTTTLMALAFAIPLAALVSWLASISLTRPVSAIARVAQRYAAGDLSRSTFHY
                     GADEIGTVARALDGSVQQLAGRIDDLARDRARMEAILGGMVEGVLVVDRQGRLQLVNR
                     AAQEMLRVEPSAGGRLYLEVIRHPDIAAQLTAALGGSEVGSQELSLARDPGRTFVARA
                     APVSGPSGGAVLVLHDITDLRRADQIRRDFVANVSHELRT"
     gene            complement(1178..>1849)
                     /locus_tag="AC16CY_15585"
     CDS             complement(1178..>1849)
                     /locus_tag="AC16CY_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3355270.1"
                     /translation="ILVAEDDRDIADLIARYLEKAGWTAHVAGAGDEALSHVRTHGAD
                     LVVLDVMLPVMSGLEVCRALRGDRTTAAIPIIMVTARAEETDRIVGLDLGADDYVSKP
                     FSPNELVARIRALMRRSTRSAPEASTIVFGTLVMDLGRHTVLDEGREVKLTAKEFLLL
                     QYLMQHRGRVLSRDLLLGDVWGYRYTGGTRTVDVHVRRLREKLPILVDALVTVKQFGY
                     KLEEQ"
BASE COUNT          292 a          651 c          628 g          278 t
ORIGIN      
        1 gtccgcagct cgtgcgacac gttggcgacg aagtcccgcc tgatctgatc ggcacggcgc
       61 aggtcggtga tgtcgtgcag cacgagcacg gcgccgccgc tcggtcccga caccggcgcg
      121 gcacgcgcga cgaatgtgcg ccccggatcg cgcgccagcg agagctcctg gctgcccacc
      181 tcgctgcccc cgagtgccgc ggtgagctgg gcagcgatgt ccggatgacg gatgacctcg
      241 agatacagcc ggccgccggc cgacggctcc acccgcagca tctcctgcgc cgcccggttg
      301 acgagctgaa gccggccctg acgatcgacg accagcacgc cctcgaccat gccgccgagg
      361 atggcctcca tgcgggcgcg atcccgggcc agatcgtcga ttcgaccggc caactgctgc
      421 acggatccgt cgagagcccg ggcgacggtt ccgatctcgt cggccccgta gtgaaaggtg
      481 gagcgcgaca aatcgccggc cgcgtagcgc tgggccacac gtgcgatcgc gctgaccggc
      541 cgggtcagcg agatcgaggc cagccacgag accagcgcgg cgagagggat cgcgaaggcc
      601 agagccatga gcgtggtcgt gcggatcgtg gcgagctgcg cgtcgatatc cgtgagcggc
      661 agtgccagac gtacgtagcc caccaacggg tgcgatgcgc gtacggcgac gtagaccatg
      721 cccacgccga ccgtggtgct gtacctgtgg ctgctgccga ccccgttctc ccgcgcggca
      781 gccacttccg gccgtgacgc gtggttgtcg atctcgagca gcgcggccgg cgactgcgtc
      841 gagtccccga gcacacggcc gtcgcgcgcg atgaatgtga cgcggccggg aaccagctcg
      901 ccaaggcgat ccgcttcctc atcgagctgg tcttcgccaa tcgacgtggc cctggtcagg
      961 agctctgcga tcagatgcgc ctggtcggtc agccgctgct cgatggcggc ccgctgccgt
     1021 tcgcgcacct gccacgacac caggacggcc agcgcgagca gcgacacgcc cgcggcggcg
     1081 aaggatccga cgaagacctt ggtgcgaaag ccgatcttca tgctttcagc cagtggccgg
     1141 cagccggtcg ccggtggacg gcagctccct caccggttca ttgttcttcc agcttgtaac
     1201 cgaactgttt cacggtcaca agggcgtcca ccaggatcgg cagcttctcg cgcagccggc
     1261 gcacgtgcac gtccaccgtc ctcgtaccgc cggtataccg gtacccccac acgtcgccga
     1321 gcagcaggtc cctcgagagc acgcgcccgc gatgctgcat caggtactgc aggagcagga
     1381 attcctttgc cgtcagcttg acctcgcgtc cctcgtccag cacggtgtgg cggcccaggt
     1441 ccatcacgag cgtgccgaag acgatggtgg acgcctcggg cgccgatcgg gttgagcgcc
     1501 gcatcagcgc ccgaatccgc gcgaccagct cgttggggct gaacggcttg gagacgtagt
     1561 cgtcggcgcc gagatcgagg ccgacgatcc gatcggtttc ctcggcccgc gcggtgacca
     1621 tgatgatcgg aatcgccgcg gtcgtgcggt ctccccgcag cgcgcggcac acctcgaggc
     1681 cgctcatgac ggggagcatg acgtcgagca ccacgaggtc cgcgccgtgt gtccggacgt
     1741 gggacagcgc ctcgtcgccc gccccggcga catgggcggt ccagcccgcc ttctcgaggt
     1801 agcgcgcgat cagatcggcg atgtcgcggt cgtcttcggc gacgaggat
//
LOCUS       DBNGSH010000675         2869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3636799, whole genome shotgun sequence.
ACCESSION   DBNGSH010000675 DBNGSH010000000
VERSION     DBNGSH010000675.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3636799"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..423
                     /locus_tag="AC16CY_15590"
     CDS             118..423
                     /locus_tag="AC16CY_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355271.1"
                     /translation="MVVRISPNDRNNPPGKLADAEVHFTDGAFEGLKLIGFGVWERRT
                     GGGRNVTFPARQYSVNGERRTFALLRPATGDATAQDRVRELVLQAYAEYEQQSHEAG"
     gene            complement(432..2195)
                     /locus_tag="AC16CY_15595"
     CDS             complement(432..2195)
                     /locus_tag="AC16CY_15595"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3355272.1"
                     /translation="MKQSWPQRERARELLSKEVGYVRKPHGGRLRVALAFPNTYFVGM
                     SNLGFQTVYTLFNAEADIVCERVFLPARSEVDALRASGAALLSLESQTPVREFDIFAL
                     SVSFEWDYTNVLTLLRLAGIPIRAADRTDRDPLVMIGGAVTFVNPEPLAPFADVISAG
                     EGEALIPPLVGAFRNSNGRDQLLRRLAGERGFYVPSFYDVRYSGDGTIEAFVPKDGTA
                     APPVVRKAAIATTEALDPPATTIFTPDTEFGSRLLVEVVRGCANLCRFCWAGYNYLPV
                     RAFPKDRILQLAERARPHSRRIGLVSIALCDHPDIEEILTRLVEMGYSISPASLRLDD
                     LTPTILRLLRESGERTITIAPETGSDRLRRVINKTITNDEILAAADLIFATGFENLKL
                     YFMIGIPTETDEDLVAIHDLTRQLRDSMLRHARPRGQIGRIVGSVNPLVPKPGTAYQW
                     LPMEADDSIERKIARMRSLMSGIDNVYFNIKSERHSFYQALLSLGDRRVAPAIEAAER
                     NGGNWRAAVKEAGVDAGFFVFRDRSADAVLPWDIIDGGLKESFFRSEADKALRAEWTL
                     PPKRQRENARLLAPLLLVDGG"
     gene            complement(2481..>2869)
                     /locus_tag="AC16CY_15600"
     CDS             complement(2481..>2869)
                     /locus_tag="AC16CY_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355273.1"
                     /translation="VVDPQLTGRVKLTVIATGFDREVSRGAASETPVDLNIYTAHLNQ
                     EGEAARSASASRSRPLATAAAEPASTPLAGLHMPASPALGVTRRAGVELSMLGAGAGS
                     PPVSAGDSAGEVSSPLDVPAFLRRQN"
BASE COUNT          483 a          947 c          998 g          441 t
ORIGIN      
        1 ttgccaccac gcgcatcgct cgccttccgg ggcgcgctcc ttgctcgagc acgccacgcc
       61 agcgcactcg atcttgcgcg cagcggcgag cgagcggtca ccgcagagga gggccacgtg
      121 gtcgtacgaa tttcacccaa tgacaggaac aacccgcccg gcaagctcgc ggacgcggaa
      181 gtccacttca cggatggcgc attcgaaggg ctgaagctga tcggcttcgg cgtgtgggag
      241 cggcgcaccg gcggcggacg caacgtcacg ttcccggcgc ggcagtactc ggtcaacggc
      301 gagcgccgga ccttcgcgct gctcaggccg gccaccggcg acgccaccgc ccaggatcgc
      361 gttcgcgagc tcgtcctgca ggcgtacgca gagtacgagc aacagtcgca cgaggctggt
      421 tagtggaaca gctatccgcc atccaccaac aagagaggag caaggagcct ggcgttctcg
      481 cgctggcgct tgggcggcag ggtccattcc gccctgaggg ccttgtcggc ctcggacctg
      541 aagaaactct ccttcaggcc gccgtcaatg atgtcccagg gcagcaccgc gtcggccgat
      601 cgatcgcgaa agacgaagaa gccggcgtcg acaccggcct ccttcacggc ggccctccag
      661 ttgccgccgt tgcgctcggc ggcctcgatg gcaggtgcga cgcggcgatc gccgagcgag
      721 agaagggcct ggtagaagga atgccgctcc gacttgatgt tgaagtagac gttgtcgatg
      781 ccactcatga ggctccgcat ccgcgcgatc ttgcgctcga tggagtcatc ggcctccatc
      841 ggcagccact gataggcggt ccccggcttc ggcaccagcg ggttcacgct gccgacgatg
      901 cggccgatct gcccgcgcgg gcgggcgtgc cgcagcatcg agtcgcggag ctggcgcgtg
      961 aggtcgtgaa tcgccaccag gtcttcgtcg gtctcggtcg ggatgccgat catgaaatac
     1021 agcttcaggt tctcgaaccc cgtggcgaag atcaggtcgg ccgcggcaag aatctcgtcg
     1081 ttcgtgatgg tcttgttgat cacgcgtcga agccggtccg acccggtctc aggagcgatc
     1141 gtgatcgttc gctcgccgct ctcacgaagc aggcgcagga tggtgggcgt gagatcgtcg
     1201 agccggagcg acgccgggct gatgctgtag cccatttcga ccaggcgcgt gaggatctct
     1261 tcgatatccg ggtgatcgca caaggcgatc gagacgagcc cgatgcgccg cgaatggggc
     1321 cgggcccttt cggccagctg caggatgcgg tccttgggga acgcgcgcac cggaaggtag
     1381 ttgtatccgg cccagcagaa ccggcagagg tttgcgcatc cgcggacgac ctcgacaagg
     1441 agcctcgagc cgaattcggt gtctggcgtg aatatcgtcg tcgcaggagg atcaagggct
     1501 tccgtcgtgg cgatggccgc cttgcgcacg acgggcggtg cggccgtccc gtccttcggc
     1561 acgaacgcct cgatggtgcc gtcgccgctg taccgcacgt cgtagaacga aggcacgtag
     1621 aagccccgct ccccggcgag gcggcgcagc agttgatccc ggccgttcga gttccggaac
     1681 gcgccaacga gcggtggaat caacgcctcg ccttcaccgg ccgaaatcac gtcggcaaac
     1741 ggcgccagcg gctccgggtt cacgaacgtg acggccccgc cgatcatgac gagcggatca
     1801 cgatcggtgc gatcggccgc gcggatcgga atgccggcca ggcgcaacag cgtcaggacg
     1861 ttggtgtagt cccactcgaa tgagaccgag agggcaaaga tgtcgaactc ccggacgggg
     1921 gtttgcgact cgagggacag gagcgccgcg cctgacgcgc gaagggcgtc gacctcgctc
     1981 ctggccggca ggaacacgcg ctcgcacacg atgtccgctt cggcgttgaa gagcgtgtac
     2041 accgtctgga agcccaggtt ggacatcccg acgaaatagg tgtttgggaa cgcgagcgcc
     2101 acgcgcagcc ggcctccgtg cggcttgcgc acgtagccca cttccttcga caggagctct
     2161 cgagcccgct cgcgctgagg ccaggattgc ttcatgaaac cttgggagtc gataccggaa
     2221 aacgggcgat cactggcgcg ggagcttcgc ctgacgcgcc ggcgctcccg cgtaccgtgg
     2281 gtgtatgcag tgtaccaccg gccggcgccg gcgactgcgc gcggagcgcg ccaagccggg
     2341 gtccccaacg cgcggattgt gcgcgttggg gtggaagaga tactgcctgg ccagccaggg
     2401 gctctgccgg gctctcaccc ctcgactcgg ctcggggtgc cctgagcgtc agtcgaaggg
     2461 caagagcgcc cctggctggc tcagttctgc cggcgcagaa aggcgggcac gtcgagcggc
     2521 gagctcacct cgcccgcgct gtcgcccgcc gacacggggg gtgagcccgc acccgcgccc
     2581 agcatcgaca gctcgacgcc cgcacgcctg gtcacgccga gcgcgggaga ggccggcatg
     2641 tgaaggcccg ccaggggcgt ggacgccggc tcggcggcag cggtcgccaa gggccgcgag
     2701 cgcgacgccg aggcgctgcg cgcggcttcg ccttcctggt taaggtgtgc cgtgtagatg
     2761 ttcaggtcca ccggcgtctc cgacgcggcc ccgcgcgaga cctcgcgatc gaacccggtc
     2821 gcgatcacgg tgagcttcac gcgtcccgtc agctgcggat cgacgacgg
//
LOCUS       DBNGSH010000676         3337 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2957512, whole genome shotgun sequence.
ACCESSION   DBNGSH010000676 DBNGSH010000000
VERSION     DBNGSH010000676.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2957512"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="AC16CY_15605"
     CDS             complement(<1..769)
                     /locus_tag="AC16CY_15605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3355274.1"
                     /translation="MTFDLGLAGYRALVTAGTKGVGAAVVEVLRQNGARVMTTARSVP
                     QGARDDVHYIAANLTVPADCVSVASSALDHFGSVDIVVNVLGGSQAPAGGFAALDDDE
                     WRKELDLNLMSAVRIDRALLPSLIAQGSGVIVHVTSIQHELPLPESTTAYAAAKAALS
                     TYSKALSKEVTPKGIRVVRVSPGWVETDAAVALAERLAAEAGTDYDGGKQIIMKSLGG
                     IPLGRPAKPREVAELIAFVTSPRAAAITGTEFVIDGGT"
     gene            complement(766..1107)
                     /locus_tag="AC16CY_15610"
     CDS             complement(766..1107)
                     /locus_tag="AC16CY_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3355275.1"
                     /translation="MGTNAKPVNLPPSVAAYFQADTRDADRVAQCFTADAVVIDERRE
                     HLGRAAIANWKTEATAKYHYTSEPIGIQTAGKELTVTARLVGDFPGSPVKLRYRFTLE
                     GDKIARLEITA"
     gene            1173..1523
                     /locus_tag="AC16CY_15615"
     CDS             1173..1523
                     /locus_tag="AC16CY_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3355276.1"
                     /translation="MSKRSHTAASAATGIEQAIRMLEGRWKLLILFHLFGGKTLRFSD
                     LERAIPAVSQKMLTQQLRRLERDGLVARTVHPEVPPRVEYHLTDWGQSLCPALDKLLT
                     WAEGQPTTGGPQKP"
     gene            complement(1616..>3337)
                     /locus_tag="AC16CY_15620"
     CDS             complement(1616..>3337)
                     /locus_tag="AC16CY_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3355277.1"
                     /translation="AVFRNARFGPQIQPLKQEIVGNVGAMLWVVMGTLAIVLLIACAN
                     VASLLLVDAEVRGPELAVRAALGAGWRRIAGQLLAESMTLGLLGGALGLGPAYAGLRI
                     LAARGPSTLPRLDEISFDPWVLAFAFVASLLSGALAGIIPLLKYARPPAAALRTVGRT
                     FSQTRERHRARNTLVVVQVALALVLLVGAGLMIRTFQQLRAVQPGFTQPETIHIVRSS
                     IPEAIAKEPARAMRMWQEILDKIAAIPGVTSVGLAGQVPLESQLGFRNRQRLDAERPT
                     LEGRPRNTSVEMRLIAPSFFETMGTRIIAGRDFTWPDLYEHRHVAIVSDNLARAWWGE
                     PRAALGQRVRESSIAPWREVVGVVEDVYDDGVHVPAPEFAYLPALMDRHLVFDSEFVL
                     RGATFAIRSERAGTESLLGEARNAIWSVNGRQPVFSFVNTVGQLYDQSMVRTSFTLVI
                     LAIVGGMALLLGIVGIYGVIAYAVSQRTREIGLRMALGAQPATMLPVFIRQALLLAAI
                     GVGVGLAAAVGLTRLMSSLLFGVSAVDPLTYAAVSALLMAAAVLASYVPARRAMAIDP
                     VEALRAE"
BASE COUNT          623 a         1130 c         1047 g          537 t
ORIGIN      
        1 cagtcccacc atcgatgacg aactcggtgc cggtgatcgc agccgctcga ggcgaggtca
       61 caaaggcgat cagctcggcg acctcacgcg gcttcgcggg ccgtccgagc ggaatcccgc
      121 caagcgactt catgatgatc tgcttcccgc cgtcgtagtc ggtcccggct tcagcggcca
      181 ggcgctcggc gagcgccacc gcggcatccg tctccaccca ccctggcgag acccgcacga
      241 ctcgaatccc cttgggtgtg acttcctttg agagggcctt gctgtacgtc gacaacgcgg
      301 ctttcgcggc ggcgtaggct gtcgtcgact cgggaagggg cagctcgtgc tgaatcgacg
      361 tcacgtgaac aatgacaccg gacccctgag cgatcagcga cggtaacaac gcgcgatcga
      421 tccgcaccgc ggacatcaaa ttcagatcga gttcctttct ccactcgtcg tcgtcgagcg
      481 ccgcgaaccc tccagccggc gcttgagacc cgccaagcac gttgacgacg atatcgacac
      541 taccaaagtg atccagcgca ctgctggcga cactgacgca gtccgcaggg actgtgaggt
      601 tcgccgcgat gtagtgcaca tcgtcgcgag cgccctgcgg gaccgaccgt gccgtcgtca
      661 tcacgcgcgc accgttttgg cgcagcacct cgacgacagc ggcccccaca cccttcgttc
      721 cggccgtgac gagcgcgcgg tacccggcga gcccgagatc gaacgtcacg cggtgatctc
      781 gagccgggcg atcttgtcgc cttcgagcgt gaagcggtac cgcagcttca ccgggctgcc
      841 tggaaagtct ccaaccagcc gggcggtcac cgtcagttct ttccccgcgg tctggattcc
      901 aatcggctcg cttgtatagt gatacttcgc agtggcctcg gtcttccagt tcgcgatggc
      961 cgctcggccc aggtgttcgc ggcgctcgtc gatcacgacg gcgtccgcgg tgaaacactg
     1021 cgccacacga tcggcgtcac gcgtgtccgc ctggaagtag gcagcaacgg atggaggcag
     1081 gttgactggc ttcgcgttcg tccccatgcc ggtactacac atctcgccat ggccaggacg
     1141 caagaacgca cgcgaaggtg ggggactcac ctatgagtaa gcgctcgcac accgccgcgt
     1201 ccgccgccac aggtatcgag caggcgatta gaatgctcga ggggaggtgg aagcttctga
     1261 tcctctttca tctgtttggg ggaaagacgc tgcggttctc cgacctcgag cgtgcgattc
     1321 ccgccgtgtc gcagaagatg ctgactcagc aactccggcg actcgagcgc gacggcctcg
     1381 ttgcacgcac cgtgcatccc gaggttccgc cgagggtcga gtatcacctt accgattggg
     1441 gccagtccct ctgcccggcg ctcgacaagt tgctgacctg ggccgaggga caaccgacga
     1501 cgggcggccc tcaaaagcct tgaccgtgag gacccgcaat gcgccgatgc gtaatcaggc
     1561 cgcatgaatc agccagttgc ggaatcgtcc cgaacagcca gccagctccc ttgtcctatt
     1621 ccgcgcgcag ggcttcgacc ggatcgatgg ccatcgcgcg gcgtgcgggt acatagctgg
     1681 ccagtacggc cgcggccatc agcagggctg acaccgccgc atacgtcaac gggtcaacgg
     1741 cggacacacc aaacaacaac gatgacatca aacgtgtcag ccccaccgca gctgccagcc
     1801 ccacgccgac tccaatagca gccagcagca gtgcttgccg tatgaacacc ggcaacattg
     1861 tggccggctg cgcaccgagc gccatgcgga ggccgatctc gcgagtccgc tgcgagacgg
     1921 cataggcgat cacgccgtag atgccaacga tcccgagcag aagagccatc cctccgacaa
     1981 tcgccagaat gaccagggta aacgaggtac gcaccatgga ctggtcgtag agttggccga
     2041 cggtgttcac gaaagaaaag accggctgcc gtccattcac cgaccagatg gcgtttcgcg
     2101 cctcccccag caggctctcg gttccggctc gctcggagcg gattgcgaac gtcgccccgc
     2161 ggaggacgaa ttcactatcg aagaccaggt gtcggtccat cagcgcaggg aggtacgcga
     2221 actccggcgc tggtacgtgc acgccgtcgt cgtacacgtc ttccacgacg cccaccactt
     2281 cgcgccaggg ggcgatcgag ctttcacgaa cgcgttgtcc gagcgcagca cgcggctcgc
     2341 cccaccacgc acgcgcgaga ttgtcggaca cgatcgccac gtgccggtgc tcgtagagat
     2401 ccggccaggt gaaatccctt ccggcgatga tccgcgtccc catcgtttcg aagaatgacg
     2461 gggcaatcaa tcgcatttcg accgaggtat tgcgtggacg gccttcgagg gttggccgtt
     2521 cggcgtcgag ccgctgccgg ttcctgaagc ccaactggga ctccaggggg acctggccgg
     2581 ccaacccgac cgaagtgacg cctggaatgg cggcgatctt gtcgaggatc tcctgccaca
     2641 tccgcatcgc acgcgctggc tccttggcga tggcttccgg gatcgaggag cggacgatgt
     2701 ggatcgtttc cggctgcgtg aatcctggct gcaccgcgcg cagttgctga aacgtgcgaa
     2761 tcatcaggcc ggcaccgacc agcagcacga gcgcgagcgc aacctgcacg acgaccagcg
     2821 tgttgcgcgc gcgatggcgt tcgcgggtct ggctgaaggt gcggccgacg gtccgcagcg
     2881 ccgcagcggg cggccgcgcg tatttcagga gcgggatgat tccggcgagc gcgcccgaga
     2941 gcagcgatgc cacgaaggcg aaggccagca cccaggggtc gaaggagatt tcgtccaggc
     3001 gcggcaacgt gctgggacct cgtgcggcca gaatcctgag ccccgcatac gccggcccga
     3061 gacccagggc gccaccaagc agtcccagcg tcatgctctc ggcaagcaac tgccccgcga
     3121 tccggcgcca gccggcgcca agcgccgcac ggaccgccag ttccggtccg cgaacctcgg
     3181 cgtccacgag caggaggctg gcgacgttcg cgcaggcgat cagcagcacg atcgcgagcg
     3241 tccccatgac cacccagagc atcgcgccca cgttgccgac gatctcctgc ttcaacggct
     3301 ggatctgcgg tccgaagcgg gcattgcgga agacggc
//
LOCUS       DBNGSH010000677         1777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3807486, whole genome shotgun sequence.
ACCESSION   DBNGSH010000677 DBNGSH010000000
VERSION     DBNGSH010000677.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3807486"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="AC16CY_15625"
     CDS             complement(<1..941)
                     /locus_tag="AC16CY_15625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3355278.1"
                     /translation="MIDNVPKHAAAILMIAYTNYETDPRVIRAAEAAAEAGFAVDVLA
                     LRRPNQPAEQVRHGVRILRVPQQRYRGRSRIGYVLAYGAFFCRCVAASTRLFITRRYR
                     VIHVNNMPDALVFSVVIPRLLGAKVILDIHDPMPETLGSKYSGPSSSALYKGLLFLER
                     LSVAFANRTITVNHPVRDTLVVKRGYRPQTIDVVANFADDQLFKPMPYPAIEGRLRFV
                     FHGTILERYGLRTLVSAVAQAKHKDKIQIRLIGEGDFSAELKRLIKDHNLEDVIEFVN
                     RVYPLHEIPAVLSDCHVGLVPLDVTPISDVALPLKLL"
     gene            complement(987..>1777)
                     /locus_tag="AC16CY_15630"
     CDS             complement(987..>1777)
                     /locus_tag="AC16CY_15630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3355279.1"
                     /translation="KAIIPVHLYGQSADMRTVLDVAKARHVVVIEDAAQAIGAEFDGR
                     RCGAMAEYGCFSFFPSKNLGAGGDAGMVVTNDPARAATLAILRAHGSKPKYYHRVVGG
                     NFRLDALQAAIVSAKLPHLDGWTEGRRRNAARYDRLFTESGLSVCHSREWVDGGRRGM
                     PDVVLPSAVTDRHVFNQYVIRVAARDELKTRLQEKGVGTEIYYPVPMHQQECFASLGY
                     RAGDFPESEMAASQTLALPIYPELTDEQASYVVDCIRGFYGRPD"
BASE COUNT          333 a          556 c          561 g          327 t
ORIGIN      
        1 tccagaagtt tcagaggcag ggcgacgtcg gagatcggcg tcacatcgag cggcaccagc
       61 ccgacgtggc agtccgagag aaccgcggga atctcgtgca aggggtacac gcggttcacg
      121 aactcgatca cgtcctcgag attgtggtcc ttgatcagcc tcttgagctc ggcgctgaag
      181 tcgccttcgc cgatgagccg gatctgaatc ttgtccttgt gcttcgcctg tgccactgcc
      241 gacacaagcg tgcgaagccc atagcgctcg agaatggttc catggaaaac gaagcggagg
      301 cggccctcga tcgccggata cggcatcggc ttgaacagct gatcatctgc aaaattcgcc
      361 acgacatcga tcgtctgagg tcgatacccc cgcttgacca ccaacgtgtc tcgtaccggg
      421 tggttgaccg tgatcgtccg gttggcaaag gccacactga gccgctccag gaacaggagt
      481 cccttgtaca aggcgctgga actcggtccc gagtacttcg aacccaacgt ctccggcatg
      541 ggatcgtgaa tgtcgaggat gaccttcgcg ccgagcagtc gcggtatcac gaccgagaac
      601 accagggcgt ccggcatgtt gttcacatgg atgacgcgat agcggcgagt gatgaagagt
      661 cgcgtagacg ccgcgacgca ccgacagaag aacgccccgt atgcgagcac gtacccgatc
      721 ctcgagcggc cgcgataccg ctgctgagga acacgaagga tgcgcacgcc gtgccgcacc
      781 tgctctgccg gctgattcgg acggcggagt gcgaggacgt cgacggcgaa tccggcctcg
      841 gcggctgcct cggctgcacg tatgaccctc gggtcggtct cgtagttcgt gtacgcgatc
      901 atcaggatcg cggccgcgtg cttgggaacg ttgtcgatca cgtgtgtgct ccgccgctcg
      961 gatcgtccgt cggtgtcgga gtgtcttcag tcaggccggc cgtagaaccc gcggatgcaa
     1021 tcaaccacat agctcgcctg ctcatcggtc agttccggat agatgggcaa agcaagcgtc
     1081 tgcgatgccg ccatttcgct ttcggggaag tctccggcgc ggtagccgag cgaggcaaaa
     1141 cactcttgct ggtgcatcgg tacggggtag tagatttccg tgcccacgcc tttctcctgc
     1201 agccttgtct tcagctcgtc gcgcgccgcc actcgtatga cgtactggtt aaagacgtgc
     1261 cgatccgtga cggccgacgg gaggacgacg tccggcatgc cacggcggcc tccgtcgacc
     1321 cactcgcggc tgtgacacac gctcagcccg gactcggtga agagccgatc gtagcgggcc
     1381 gcgttgcgac ggcgcccttc ggtccagccg tccagatgcg ggagcttggc cgacacgatc
     1441 gcggcctgga gggcgtcgag acggaagttg ccgccgacaa cgcgatggta gtacttgggc
     1501 ttcgagccat gcgcccgcag gatggccaat gtggccgcgc gcgcgggatc gttggtcacc
     1561 accatgcctg catcgccgcc ggcgccaagg ttcttcgatg gaaagaacga gaagcatccg
     1621 tactccgcca tggccccgca tctgcgtccg tcgaactcgg ctccgatcgc ctgggccgca
     1681 tcctcgatca ccacgacgtg acgcgctttg gcgacatcga gaacagttcg catgtcggcc
     1741 gattgaccgt acagatgaac cggaatgatc gccttcg
//
LOCUS       DBNGSH010000678         5278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2789126, whole genome shotgun sequence.
ACCESSION   DBNGSH010000678 DBNGSH010000000
VERSION     DBNGSH010000678.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2789126"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="AC16CY_15635"
     CDS             complement(<1..603)
                     /locus_tag="AC16CY_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3355280.1"
                     /translation="MHRITPAMRSLLLGATAAAIFVTTTYGQSILGPPSEANHFIETP
                     KGWAHPRTPWGEPDIQANLNMMQAAGIPLERCASSIGRGGPCDMNKAWRTPQEYKEAL
                     DAAAGRGDRGRQLLADGNFGAALLAGVVDLSTPQRQTTLIVDPPSGLLPALTPEGKRR
                     ALQMRSGWSLPGESPSYDGPGDFDSWDRCTTRGMPSSMMPY"
     gene            complement(662..1558)
                     /locus_tag="AC16CY_15640"
     CDS             complement(662..1558)
                     /locus_tag="AC16CY_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355281.1"
                     /translation="MRPLSLTARSSATLVAVGISLCVPAPALAQAWPAPANVGAVNVV
                     YQAVANTGHRLHDGSMLEGYDSYSQSVLVNVDYAVTDRFSFALGVPYVASKYTGPEPS
                     LFLLPIDECHCWNHGWQDIAGTARYTVVNGVVALTPSVSFGMPSHNYEYQGEAVLGLN
                     LNEVRVALDAGHRFAAMPRLAVSARYSYAFVENVVDVPIDRSNFAITPSYAVTRKMTV
                     TGVFAWQRTHGGLGSNEFATEEQFLQFDRLIRDNNFQMGATFARTFRHFDVFASYLDF
                     VSGTDTHDGYAITAGVSWPFER"
     gene            complement(1572..2108)
                     /locus_tag="AC16CY_15645"
     CDS             complement(1572..2108)
                     /locus_tag="AC16CY_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355282.1"
                     /translation="MSKRLSLPLLVLLGVCASADLFACGDKFLVVSRGLGLQRTGAAR
                     SAADILIYGTKASTLKTSLARIEKEDSLRKAGYRVVIVEDTSQLDEALRLNTYSWSLV
                     IADLPEARVIRDRIRGTNAPMVLPVALSPTGTEIAQAKKDFQRVVKGPIKSQAFLYEI
                     DDALALWQKLQQRSKLTV"
     gene            complement(2194..4767)
                     /locus_tag="AC16CY_15650"
     CDS             complement(2194..4767)
                     /locus_tag="AC16CY_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355283.1"
                     /translation="MDQWEQVSGIVRTTLALEPHARGAYLDATCAGNDALRRQVDALL
                     ATADDTRTVASPLRLLPSGSVFAHYRILGELGAGGMGVVYRARDERLDRNVAVKVLPP
                     SAFGDPTARARLVREAKAAAALNHPNVCTVHEVGEANGQAYIAMELVEGETLTAKLAA
                     RPLSLGRLLNYGRQLADALTHAHDRGVVHRDLKSNNVIVTPDERVKVLDFGLAKRLSD
                     ADLGAAATETQQSLTQPGTAAGTLPYMSPEQLRGGAARSSSDIWALGVVLYEMASGLR
                     PFTGQTAFEVSAAILGQSPPPLPSTLPPALDELIARCLEKEPDRRFRTAAELRTALEA
                     IPATAGPAPTDVETRPAAPVGSQARARVVHLPSRRAVLISAAVALVVVGTAGAWRFWP
                     RPVRTLAVLPFVNVSKDADTEYLSSGIAEALIVQISRQATIAVSNLPTVLPFKDRVSD
                     PGGVGRELGVATVVTGSVEQQGAQLLVAAQLLDVGNGQQLWSGRYDRKAVELLDVQNE
                     VALAIVNALRVRMSRADQQRLVKHPTTDGDAYDLYLQARHIQRSATEEDYLYSRELLQ
                     RAVLRDPKFALGYAALSGNHAMMVVDGLERPIDAWAEVSKYMNRALAIDPTLPDIDAY
                     AHAMAFLWLWDWAETERARRRVLQYPLRELDPHVLRALAVEHWALGRPAEALQLARRM
                     RELDPSSPYLGTLEADYLINNEQYDAAVPLYERAIRLDPTNLNSYFGLAEARAGQRRF
                     DEAIEARRQVHRIAGDEALNDVLATAKGEEGYRAIQRRWVLVQLEMLKARQATSYTSP
                     LEFARVYAQLGDKEQAFKHLDAAFLDRSPGLVFLKVDRAWDLVRDDSRFLEAIRKVGL
                     P"
     gene            complement(4825..5097)
                     /locus_tag="AC16CY_15655"
     CDS             complement(4825..5097)
                     /locus_tag="AC16CY_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355284.1"
                     /translation="MTSDDLGVAASSHLASLPRAAPDPVRAERTRLRCRARLVRRQRR
                     RIHATSTIALARRVLTAVAVGGFCAFCVFYVGSLVEATLRFGGVLR"
     gene            complement(5094..>5278)
                     /locus_tag="AC16CY_15660"
     CDS             complement(5094..>5278)
                     /locus_tag="AC16CY_15660"
                     /inference="COORDINATES: protein motif:HMM:NF019886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR3355285.1"
                     /translation="YREALLLVVVEGLRQSEAAAICGVTRVAMRQRVSRARMRLARRL
                     AETDGPGLASLNEVTT"
BASE COUNT          887 a         1734 c         1782 g          875 t
ORIGIN      
        1 atacggcatc atcgacgacg gcatgccgcg cgtcgtgcag cggtcccagc tgtcgaagtc
       61 gccaggcccg tcgtaggaag gcgactctcc gggcagcgac cagccgctgc gcatctggag
      121 cgcgcgccgc ttgccctccg gcgtcagggc cgggagcagt ccgctgggcg gatcgacaat
      181 gagcgtggtc tgccgctgcg gcgtgctcag atcgacaacg cccgcaagga gcgccgcgcc
      241 gaagttgccg tcggcgagca gttgacggcc gcgatcgcct cggccggcag cggcgtcgag
      301 cgcctccttg tactcctgtg gcgtccgcca cgccttgttc atgtcgcacg ggccgccacg
      361 gccgatcgag ctggcgcacc gctcgagcgg gatgccggca gcctgcatca tgttcaaatt
      421 ggcctggatg tcgggctcgc cccacggcgt cctcggatgc gcccagcctt tgggtgtttc
      481 gatgaagtgg ttggcctcgc tcggcgggcc gagaatggac tggccgtacg tggtggtcac
      541 gaaaatcgcg gctgccgttg cgccaagcag caggctgcgc atcgccggcg tgatccggtg
      601 catggtcact cctgtggcgc agtggggcgc cagaggtagt ctctcacgac tgggcgggtg
      661 ctcagcgctc gaagggccag ctcacgccgg cggtgatggc gtagccgtca tgagtgtccg
      721 tcccgctgac aaagtccaga tacgaggcga acacgtcgaa gtgccggaac gttcgcgcga
      781 aggtggcccc catttggaag ttattgtcgc gaatgagccg gtcgaattgc agaaactgct
      841 cctcggtcgc gaactcgttc gaacccagcc ctccgtgcgt gcgctgccag gcgaacacgc
      901 cggtgacggt catctttctg gtgacggcat acgacggcgt gatcgcgaag ttgctccggt
      961 cgatcggcac gtccacgacg ttttccacga aggcatagga gtatcgcgcg gatacggcca
     1021 gccgtggcat cgccgcgaac cggtgacccg catcgagcgc gacgcgcact tcgttgagat
     1081 tgagccccag caccgcctcg ccctggtact cgtagttgtg gctgggcatg ccgaacgaca
     1141 cggacggcgt caatgccacg acgccgttca cgactgtgta gcgtgccgtg ccggcaatgt
     1201 cctgccatcc gtgattccag cagtggcact cgtcgatcgg cagcaggaac agactgggct
     1261 cggggcccgt gtatttcgac gccacataag gaacgcccag tgcgaacgag aatcgatcag
     1321 tgaccgcgta gtcgacgttg acgagcacgg actgactgta gctgtcgtat ccctccagca
     1381 tgctgccgtc atgaaggcgg tggcccgtgt tggccacggc ctgatacacg acgttcaccg
     1441 cgccgacgtt tgcaggtgcc ggccacgcct gcgcgagcgc cggcgcgggg acgcacaggc
     1501 tgatgccgac ggcgaccagc gttgcgctag atcgcgcggt gagagacagg ggacgcatcg
     1561 agatccgaag gtcagacggt cagcttcgag cgctgctgca gtttctgcca cagggcgagg
     1621 gcgtcgtcga tctcatacag gaacgcctgg ctcttgatcg gccccttgac gacgcgctgg
     1681 aagtccttct tcgcctgcgc gatttcggtg ccggtcgggc tcaacgcgac cgggagcacc
     1741 atcggcgcgt tcgtgccgcg gatacgatcg cggatcaccc gggcctcggg gaggtcggcg
     1801 atgacgagcg accaggagta ggtgttcagc cgcaaggcct catcgagctg actggtgtcc
     1861 tcgacgatca ccacgcggta gcctgctttt cgaagcgagt cctccttctc gattcgcgcg
     1921 agcgacgttt tcagcgtcga ggccttggtg ccgtagatga gaatgtcggc cgccgatctc
     1981 gccgcgcccg tgcgctggag tcctaggcct cgactgacga cgaggaactt gtctccgcag
     2041 gcgaacaggt cggcgctcgc acacacgccc aatagcacga gtaaggggag ggacagccgc
     2101 ttggacatga ctcgactcct agctgaacaa tgactactag cggaccgtag cacccggccc
     2161 aacacgtgtc aagacctcga caatatgcag cggctacgga agtccgactt tgcggattgc
     2221 ctcgaggaac cgggagtcgt cccgcacgag atcccaggcg cgatcgacct tcagaaacac
     2281 caggccggga gaccggtcga ggaacgcggc gtcgaggtgc ttgaaggcct gttccttgtc
     2341 gccgagctgc gcatagactc tggcgaactc caggggcgat gtgtagctcg tggcctggcg
     2401 tgctttgagc atctcgagct ggacgagcac ccaccgacgc tggatcgcgc gatacccctc
     2461 ttcccccttg gccgtcgcga gcacgtcgtt gagggcctcg tcgcccgcga tgcggtggac
     2521 ctggcgtctg gcttcgatcg cttcgtcgaa ccgacgctga cctgctctcg cttcggccaa
     2581 gccaaaatag gagttcaggt tggtagggtc caggcgtatc gcccgctcgt acaagggcac
     2641 agcagcatcg tattgttcgt tattgatcag atagtccgct tccagcgtgc cgaggtacgg
     2701 actggacggg tccagctcgc gcatccgccg ggcgagttgc agcgcttcgg ccggccggcc
     2761 gagcgcccag tgctcgacag ccagcgctcg caagacatgg gggtccaatt cccgtagcgg
     2821 gtattggagg acccggcggc gcgcccgctc ggtctcagcc cagtcccaga gccacagaaa
     2881 ggccatggcg tgcgcgtagg catcgatgtc gggcagcgtg ggatcgatgg cgagcgcccg
     2941 gttcatgtac ttgctgacct ccgcccacgc gtcgatcggc cgctcgaggc catcgaccac
     3001 catcatggcg tggttgccgc tgagcgccgc gtagccgagc gcgaacttcg gatcacggag
     3061 gaccgcgcgc tgcaggagct cgcgcgaata gagatagtcc tcctcggtgg cggaccgctg
     3121 gatgtgtcgg gcctgaagat acaagtcgta cgcgtcgcca tccgtcgttg gatgcttgac
     3181 gagacgctgc tgatctgccc gtgacatccg gactcgcagg gcgttgacaa tcgccagggc
     3241 gacttcgttc tgtacgtcga ggagctcgac cgccttgcga tcgtaccggc cgctccagag
     3301 ttgctggccg ttcccgacgt cgaggagttg agccgccacc agcagctgcg ccccctgttg
     3361 ctcgaccgag cccgtgacga ccgttgcgac gccaagctcc cggcccacgc ctcccggatc
     3421 gctcacccgg tctttgaacg gcagtaccgt agggaggttg ctgacggcga tcgtcgcctg
     3481 cctgctgatt tgcacgatca gcgcttcggc gatgccggac gacaagtatt ccgtgtccgc
     3541 gtccttcgag acattgacga atggcagcac cgcgagcgtg cgaaccggac gcggccagaa
     3601 gcgccatgca ccggcggtac cgacgaccac cagcgcgact gcagcagaga tgaggacagc
     3661 gcgccggctc ggaaggtgca cgactcgggc tcgtgcctgc gagccgaccg gcgccgccgg
     3721 ccgcgtttcc acatccgtcg gcgcagggcc agcggttgcg gggatggcct cgagcgcggt
     3781 gcgcagttcc gctgccgtcc ggaagcggcg gtccggctcc ttctccaggc agcgcgcgat
     3841 cagttcatcc agcgcaggtg gaagcgtcga gggaagcgga ggggggctct ggccaagaat
     3901 ggcggcgctg acttcgaacg ccgtttgacc cgtgaacggc cgaaggccgg aggccatttc
     3961 gtacagcacc acgcccagcg cccagatgtc gctcgagctc cgcgccgccc cgccgcggag
     4021 ctgttccggg gacatgtacg gcagcgtgcc cgccgccgtc ccgggctggg taagcgactg
     4081 ttgcgtctcg gtcgcggcag cgccgaggtc ggcatcgctc aagcgcttgg ccagtccgaa
     4141 gtcgagcacc ttgacgcgct catcgggcgt gacgatgacg ttgttgctct tgagatcgcg
     4201 gtgcacgaca ccgcggtcat gggcgtgcgt aagggcgtcg gcaagctgcc gcccgtagtt
     4261 gaggagccgg cccagagaaa gcggccgggc ggcgagcttc gcggtgagcg tctcgccttc
     4321 gacgagctcc atcgcgatgt aggcctgccc gttcgcttcg cccacttcgt ggaccgtgca
     4381 gacgttcggg tgattcaggg cggcagctgc cttggcttcg cggaccagac gggctctcgc
     4441 cgtcggatca ccgaaagcgg aaggcggcag gaccttgacg gcgacgttgc gatcgagccg
     4501 ctcgtcccgg gcccgataca ccacgcccat cccacctgcg ccgagctcgc cgaggatccg
     4561 gtagtgggcg aagaccgacc cggagggcag caggcgcagc ggcgacgcta ccgtccgcgt
     4621 gtcatccgcc gtcgcgagca acgcgtcgac ctgccgtcgc agcgcgtcgt tgccggcgca
     4681 cgtcgcgtcg aggtaggcgc cacgtgcgtg cggctcgagc gccaaggtcg tccggacgat
     4741 gcccgagacc tgctcccact gatccacggc acccctctcg ctgattgatc gacccgtgca
     4801 gccggaggga ttctaccgcg agaatcagcg aagcacgccg ccgaagcgaa gcgtcgcttc
     4861 aaccagcgag ccgacgtaga agacgcagaa cgcgcagaat ccgccgacag ccacagcggt
     4921 caacacgcgt cgcgccagcg cgatcgtcga ggtcgcatga atccgccgtc gctgccgccg
     4981 gacgagccga gcacggcagc gcaaccgcgt gcgttctgcg cgcacggggt cgggtgccgc
     5041 gcgcggcagg ctcgcgagat gcgacgaggc tgcaacgccg agatcgtcgg acgtcatgtg
     5101 gtgacctcgt tcaacgacgc aaggccaggc ccgtcggtct cggcaagccg tcgcgccagg
     5161 cgcatacgcg cccggctgac acgctgccgc atcgccactc ttgtgacgcc gcagattgcg
     5221 gcggcctcgg attggcggag cccctcgacg acgaccagca gcagcgcttc acgatagg
//
LOCUS       DBNGSH010000679         4844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2789142, whole genome shotgun sequence.
ACCESSION   DBNGSH010000679 DBNGSH010000000
VERSION     DBNGSH010000679.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2789142"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC16CY_15665"
     CDS             complement(<1..386)
                     /locus_tag="AC16CY_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355286.1"
                     /translation="MVKRTVNMVLPLAGIALALIVWWVASRLVQDLPSPARTWEESKL
                     YILEPLAKRGETDQGILRLAYYSLVRVAQGFLLGIAIATPIGFLLGVSTTLTRMFDPI
                     MQVLRPISPLAWLPLGLVIFQKSEPAA"
     gene            complement(449..1714)
                     /locus_tag="AC16CY_15670"
     CDS             complement(449..1714)
                     /locus_tag="AC16CY_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3355287.1"
                     /translation="MSKLKTQKYGRRRFLETATVSTAGVLMAGLPAGWVGGVYADDGP
                     ETSTIRFGIIALTDCAPIVMAHELGYFKKFGITSIVSKEASWAVIRDKLSLGENQATH
                     MLIGMPLASTMGLAGSPVKPMIVPWLLNRNGQAITLNNKLKQAGVRKPQDVKPLADKA
                     KASGEPLAFAMTFPTGTHAMWMRYWLAAGGVHPDKDVSLITIPPPQMVANMKVDKMDG
                     FCVGEPWNNRAIEDGIGFTVVTTQQMWKDHPEKVCAFTEEFAAKNPKTVKAILKALHL
                     ASVDLDKMENRPKFAEVISRPAYINCPPAIILERLLGKYEYGDGRVEQDPFYMIYSNR
                     TCNYPQRIYATWWLTQFRRWGMTKGAPDYAAVPKRVMRPDIYLEAMKELGVPVKVAEE
                     QSIALFDGVFDGKDPEKYARSFPINGLVG"
     gene            complement(1733..3145)
                     /locus_tag="AC16CY_15675"
     CDS             complement(1733..3145)
                     /locus_tag="AC16CY_15675"
                     /inference="COORDINATES: protein motif:HMM:NF024765.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HIR3355288.1"
                     /translation="MTTTSRISGAAAVHLAALALTASITPAQETTRAAAEPAPQAAQT
                     LAQVTQKPPEPSRAPLFNRANESLPSWFRVRGEFRERMEGFDNAGFTDPRDDLYWLSR
                     FRFDTTFTPSKMLAFQVQLQDARVGKKTVGTTGAPFSAPFDLRQGYAQVGSSTSPFAV
                     RVGRQELVYGEQRLVGHVSWLNAARSFDGAKLTVRGKAFSADVFAASLVRILDDEFDK
                     SGNGNLFAGVHGTTAKLVPKGTVEPYAFYRHDINQTGEAGASGTLTGTTVGMRMIGTL
                     PASFDYGVEMAVQRGSLSTNSSEAWAGHWQLRRSLTGAGAAKLIGEYNYASGDADPAD
                     GVRGTFDHLYPTPHDKYGLADQIGWKNIRHARVGFDFSPHRGWPVTTSYHSWWVNEKR
                     DAVYTAGGAPLARVAAGALHSHVGQEIDVQVSRALLPSLQIAGGYAHIFPGGFLKEAT
                     PGASYNYPYVMVTYVFLAER"
     gene            3772..>4844
                     /locus_tag="AC16CY_15680"
     CDS             3772..>4844
                     /locus_tag="AC16CY_15680"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3355289.1"
                     /translation="MSSLPLLTRTKDDDARFALTGRSGTPAFAPTRAIEDRMPHRAPG
                     PGEQYRFHVDMGKCIGCKCCVVACNEQNGNPASIQWRRVREIEGGWFPAVSRSYLSMG
                     CNHCLEPTCLQGCPVDAYTKDAETGIVRHSADTCIGCQYCTWNCSYGVPQYNPERGVV
                     GKCDMCHGRLSLGQTPACVGACPEGAIAIEIVDVASWRASIQDGPAIAGRPDGDISLS
                     TTRVTLPAALPPNALPVNITHVEPEHPHWPLVLMTVLTQLAVGAFTTIWLSQVLEAPA
                     NLGAAALASLLVGGLALSASTLHLGRPVHAYRAVRMWRRSWLSREVLLFSIFSMVAGV
                     YAAMLWLGVPGGSAVGAVTAAIG"
BASE COUNT          761 a         1611 c         1581 g          891 t
ORIGIN      
        1 gccgccggct cggacttctg gaagatcacc agtccgagcg gcagccacgc gagcggcgag
       61 atcgggcgca gcacctgcat gatcgggtcg aacatcctcg tcagcgtcgt cgagacgccg
      121 agcaggaagc cgatcggcgt cgcgatcgca atcccaagca ggaaaccctg cgccacgcgg
      181 acgaggctgt agtaggcgag ccgcaggatt ccctgatccg tttcaccgcg cttggccagc
      241 ggctcgagga tgtacagctt gctctcctcc cacgtccgcg ccggggatgg cagatcctgc
      301 accaaccggc tcgcgaccca ccagacgatc aacgcgagcg cgatgccggc gagcggcagg
      361 accatgttga cggtgcgttt gaccaattgt tcctccgtgt ccgcggtggc tgaagccacc
      421 gctctacatt tggcgtagcg cgcgggcttc agcccacgag accgttgatt ggaaacgacc
      481 gggcgtactt ctccgggtcc ttgccgtcga acacgccgtc aaagagcgcg atcgactgtt
      541 cttcggcgac cttcaccggc acaccgagct ctttcatcgc ctcgagatag atgtccggcc
      601 gcatgacgcg tttcgggacg gccgcgtagt cgggagcgcc cttcgtcatg ccccagcgcc
      661 ggaactgcgt gagccaccac gtcgcgtaaa tccgctgcgg gtagttgcag gtccggttcg
      721 agtagatcat gtagaaggga tcctgctcga cacgcccgtc gccgtattcg tatttgccga
      781 gcagccgttc caggatgatg gcgggcggac agttgatgta ggcgggccgg gagatgacct
      841 cggcgaactt cggccgattc tccatcttgt cgagatcgac gctggccagg tgaagcgcct
      901 tgaggatcgc cttgaccgtt ttcgggttct tcgccgcgaa ttcctccgtg aaggcacaca
      961 ccttttcggg atggtccttc cacatctgct gcgtcgtgac gaccgtgaac ccgatcccgt
     1021 cctcgatggc cctgttgttc cacggctcgc cgacgcagaa gccgtccatc ttgtccactt
     1081 tcatgttggc gaccatctga ggtggcggga tggtgatgag gctgacgtcc ttgtccggat
     1141 ggacgccgcc ggcggccagc cagtaccgca tccacattgc gtgcgtcccg gttggaaacg
     1201 tcatcgcgaa agcgagcggc tcgccggagg ccttggcctt gtcggcaagc ggcttgacgt
     1261 cctgcggctt tcgcactccc gcctgcttca gcttgttgtt cagcgtgatc gcctggccgt
     1321 tgcggttcag cagccagggc acgatcatcg gtttcaccgg cgatcccgcc agccccatcg
     1381 tcgacgcgag cggcatcccg atgagcatgt gcgtcgcctg gttctcgccg agcgacagct
     1441 tgtcgcgaat cacggcccac gaggcttcct tcgagacgat cgaggtgatc ccgaacttct
     1501 tgaagtagcc gagctcgtgc gccatcacga tcggcgcgca gtcggtcagg gcgatgatgc
     1561 cgaaccggat cgtgctcgtc tccggcccgt cgtccgcgta gacacctcca acccatccgg
     1621 ccgggagccc cgccatcagc acgccggcgg tcgagaccgt cgccgtctcg aggaaccgtc
     1681 tgcgaccgta cttttgagtt ttgagttttg acatttgagt tacaccgtcc acctagcgtt
     1741 ccgcgagaaa gacatacgtg accatgacgt aggggtagtt gtaggaagcg cccggcgtgg
     1801 cctccttcag gaagcctccc ggaaagatgt gcgcgtagcc gccggcgatc tgcaggctcg
     1861 gcaagagcgc gcggctcacc tggacgtcga tttcctgtcc gacgtggctg tgcagcgcgc
     1921 ccgcagccac acgcgccagc ggcgcgcccc cggccgtgta gaccgcgtcg cgcttctcgt
     1981 tgacccacca cgagtgatag ctggtggtga cgggccatcc gcgatggggt gagaagtcga
     2041 agcccacgcg cgcgtgccgt atgttcttcc agccgatctg gtccgccagg ccgtacttgt
     2101 cgtgcggcgt tggatagagg tgatcgaacg tgccgcgaac gccgtcggcg gggtccgcgt
     2161 cgccagacgc gtagttgtac tctccgatca gcttggcggc gcctgcgccc gtcagcgacc
     2221 gccggagctg ccagtgtcct gcccaggctt cgctgctgtt ggtgctcagg gacccgcgct
     2281 gcaccgccat ttccacgccg tagtcgaagc tggcgggcag cgtcccgatc attcgcatac
     2341 caaccgtggt tccggtgagc gtccccgacg cgccggcttc accggtctgg ttgatgtcgt
     2401 gcctgtagaa ggcgtacggc tcgaccgtgc ctttcggtac gagcttggct gttgtgccgt
     2461 ggacgcccgc gaacaggttg ccattcccgc tcttgtcgaa ctcgtcgtcc aggatgcgga
     2521 cgagcgacgc agcgaagacg tccgcggaaa acgccttgcc gcgcacggtc agcttcgcgc
     2581 catcgaagct gcgggcagcg ttgagccagc tgacgtgccc gacgagccgc tgctcgccgt
     2641 acaccagctc ctggcggccc acgcggacgg cgaacggact ggtcgacgag cccacctggg
     2701 cgtacccctg ccgcaggtcg aatggtgcgc tgaacggcgc cccggtcgtg ccgacggtct
     2761 tctttccaac ccgggcatcc tggagctgca cctgaaacgc aagcatcttt gacggcgtga
     2821 acgtcgtgtc gaatcggaag cggctcaacc agtacaggtc gtcgcgcgga tcggtgaagc
     2881 ccgcgttgtc gaatccctcc atgcgttcac gaaactcgcc gcgcacgcgg aaccacgagg
     2941 gcaggctttc gttggcgcga ttgaagaggg gagcccgcga aggctccggt ggtttctgcg
     3001 tgacttgcgc aagcgtctgt gccgcttgcg gagccggctc ggcggccgcg cgtgttgtct
     3061 cctgcgccgg cgtgatcgat gcggtgaggg cgagcgctgc cagatggacg gctgccgcgc
     3121 cagagattcg agaagttgtc gtcacgtcca cgctcccgtt cctgaacagt gatcgtcgtg
     3181 atcccacagg ggaggcaacg tggccgttcc cggcacggct tctcagccgc tgcagaccgc
     3241 acagcaagct gcgttccggc ggccgggaac ggacgcgcgc gtggagattg gcgtgtctat
     3301 gttcggcgaa cggccgcggt ggacacggca ggatgtgcct cgccagggtc cacaaacgtg
     3361 tcggacggga cggaggattc gacaattccg tcgggcttgc cgctaacggg ccgcatcggc
     3421 acgaatgcga tcggcggtcg gccagtcgtc gggcatcgaa gaccttcacg agcgcggcag
     3481 acgctgccgc gtcgcactca accggcacgg catcaggctc ttacgtcgga atctcgcgtt
     3541 cctacacgaa cgtggctttc ggcgatcgag gacacgttcg tgtcgctcgc cacccccttg
     3601 ttccgccgtc atcctcgtcg tatcgtgaga tccacgatgc gcgcccggtc gcgtgcatcc
     3661 gtccagccgc cgatcgattc ttcagctcca cgacgagccg agacgtcatc ccgctgccgc
     3721 gctgcgcgcg ggatgcacga aggttgcaga ggttctcgtg gagttatctg aatgtcgtcg
     3781 ctgcccctgc tgacgcgaac gaaggacgat gacgcgaggt tcgcgctgac cggacgcagc
     3841 gggacgccgg cgttcgcccc cacgcgcgcg atcgaggacc gcatgccgca tcgcgcgccc
     3901 ggtcccggcg agcagtaccg ctttcacgtg gacatgggca agtgcatcgg ctgcaagtgc
     3961 tgcgttgtcg cgtgcaacga gcagaacggc aatccggcct ccatccagtg gaggcgcgtc
     4021 cgcgagatcg aaggcggctg gttcccggcg gtgtcgcggt cgtacctgtc catgggctgc
     4081 aaccactgcc tcgagccgac ctgcctgcag gggtgtccgg tagacgcgta caccaaggat
     4141 gcggaaacag gaatcgtgcg gcacagcgcc gacacctgca tcggctgtca gtactgcacc
     4201 tggaactgct cgtacggagt gccccagtac aaccccgagc gcggcgtcgt cggcaagtgc
     4261 gacatgtgcc acggccgctt gtcgctcggc cagacgcctg cctgcgtggg tgcgtgtccc
     4321 gagggcgcca ttgcgatcga gatcgtggac gtcgcgtcgt ggcgcgcctc cattcaggat
     4381 ggtcccgcga tcgccggccg ccccgacggc gatatcagct tgtccacgac acgcgtgacg
     4441 ctgcccgccg cactgccgcc caacgcgctc cccgtgaaca tcacgcacgt cgagcccgag
     4501 catccgcact ggccgctcgt gctgatgacc gtcctgacgc agcttgccgt cggcgccttc
     4561 acgacgatct ggctgtcgca ggtgctcgag gcgcccgcca acctgggggc cgccgcgctc
     4621 gcctcgctcc tggtgggcgg gctcgcgctc tcggcctcga cgcttcacct gggccgcccc
     4681 gtgcacgcct atcgcgcggt tcgcatgtgg cgacggtcgt ggctgagccg cgaggtgctg
     4741 ctcttctcga tcttttcgat ggtggcgggc gtctacgcgg cgatgctctg gctcggggtt
     4801 ccaggaggat cggccgttgg cgccgtcacc gcggcgatcg gaat
//
LOCUS       DBNGSH010000680         1619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1770309, whole genome shotgun sequence.
ACCESSION   DBNGSH010000680 DBNGSH010000000
VERSION     DBNGSH010000680.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1770309"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC16CY_15685"
     CDS             <1..431
                     /locus_tag="AC16CY_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355290.1"
                     /translation="SRMARGRDVALLAIDLDGNDYWVWKAIRSFSAAVVAIEYNARCP
                     PPLAQVQEYRPDHAWNGTDYFGASLSALEKLGEEKGYRLVGCNITGTNAFFVRQDLIG
                     SQFPYELTAEHVYQPPRYDLTYDCFARVGHKTATGVYLDV"
     gene            465..1577
                     /locus_tag="AC16CY_15690"
     CDS             465..1577
                     /locus_tag="AC16CY_15690"
                     /inference="COORDINATES: protein motif:HMM:NF014927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005230 - extracellular ligand-gated
                     monoatomic ion channel activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-gated ion channel"
                     /protein_id="HIR3355291.1"
                     /translation="MKPSERSLLIAALLLLTTVPAPAAGATLAQGAQPSDEFASLERE
                     VAPHSGVPCPAESAYSLARPDSGGGPTVVGIGVFFQDVAALSDIDQTMDTDVYFVVRW
                     RDPRLADAARTAGSAECPVPTGKLWMPALEPENLRGRQSFYPDRFLVDERGVVTIGRR
                     IWAKVSYPLDFRDFPLDAHQWTLTLWPVLSKSDEVVFHPIQRMTGIADRLSIQGWRVG
                     TPQARASTAARNSRAGTYARFDVVLDVERDWIYYAWKLGLPLTLIVFMAYGVYFIPAA
                     AVPQQIGLGMTAMLTLIAYMLTLGNALPRISYLTRADWFFVGSAMLVFLGLMKAVATL
                     WLSQGPKADLIARVDTWGRGLYPIAMLANFALAFLA"
BASE COUNT          266 a          539 c          515 g          299 t
ORIGIN      
        1 tctcgcggat ggctcgcgga cgcgacgtcg cgctgctcgc gatcgacctg gatggcaacg
       61 attactgggt ctggaaggcc attcgatcgt tcagcgccgc tgtcgtcgcc atcgagtaca
      121 acgccagatg cccgcctcca ctcgcgcagg tgcaggagta ccggccggat cacgcctgga
      181 acggcaccga ctacttcggt gcgagccttt cagcgctgga gaaactggga gaggagaagg
      241 gctatcgcct ggtggggtgc aacatcaccg ggaccaacgc gttcttcgtg cgccaagacc
      301 tgatcggcag ccagttccca tacgagctga cagccgagca cgtgtaccag ccgccgcgct
      361 acgacctgac ctacgattgc ttcgcgcgcg tcggccacaa gacggcgacg ggtgtatatc
      421 tggacgtgta actcaaaact caaaaactca aaactgaaat cacgatgaag ccctccgagc
      481 gatcgttact gatcgcggca ctcctcctgt tgaccactgt tcctgccccg gccgcagggg
      541 caacgctcgc gcagggcgcg cagccttcgg acgagttcgc ctcactcgaa cgtgaggtcg
      601 cgccgcattc cggtgtgccc tgccctgccg agagcgccta ctcgctcgcc cgccccgact
      661 cgggaggcgg gccgaccgtg gtcggaatcg gcgtcttctt tcaggacgtc gcggcgctca
      721 gcgacatcga ccagacgatg gacaccgacg tgtatttcgt cgtgcgctgg cgggatccca
      781 ggctggccga tgcggcccgc accgccggat cggcggagtg cccggtgccg accggcaagc
      841 tctggatgcc ggctctcgaa ccggagaacc tgcgcggccg gcagtcgttc taccccgacc
      901 gcttcctggt ggacgagcgg ggcgtcgtca ccattggacg ccgtatctgg gcgaaggtct
      961 cctacccgct cgacttccgc gacttcccgc tcgacgctca ccagtggacg ttgacgctgt
     1021 ggccggtcct gtcgaaatcc gacgaggtgg tgttccatcc gatccagcgc atgacgggca
     1081 tcgccgatcg cttgtcgata cagggctggc gggtcgggac accccaggcg cgggcgagta
     1141 cggcggctcg aaacagcagg gccggcacct atgcccgttt cgacgtcgtg ctcgacgtcg
     1201 agcgcgactg gatctactac gcctggaagc tcggcttgcc tctcacgctg atcgtgttca
     1261 tggcctacgg cgtgtacttc atcccggccg ccgccgtgcc gcagcagatc ggcctcggca
     1321 tgacggccat gctgaccttg attgcgtaca tgctcacgct gggcaacgcg cttccgcgca
     1381 tctcgtacct gacgcgcgcg gactggttct tcgtcgggtc cgcgatgctg gtgttcctgg
     1441 gactcatgaa agcggtggcc acgctgtggc tctcgcaggg gcccaaggcc gacctcatcg
     1501 cccgcgttga cacgtggggc cgggggctgt atccgatcgc gatgctggcg aacttcgcgc
     1561 tcgcgtttct ggcgtgaggc ggcgcatggt tgacacctat acctcgatta tcgacaatc
//
LOCUS       DBNGSH010000681         1523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1940191, whole genome shotgun sequence.
ACCESSION   DBNGSH010000681 DBNGSH010000000
VERSION     DBNGSH010000681.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1940191"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1523
                     /locus_tag="AC16CY_15695"
     CDS             <1..>1523
                     /locus_tag="AC16CY_15695"
                     /inference="COORDINATES: protein motif:HMM:NF042664.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR3355292.1"
                     /translation="TPDWDIFTVGYATTLLPDVVGIWSADPDLPILASMLRTQLGTPP
                     FSQYRSLALIAHSMGGLVVQKALVDDATLGQRVRHVILFGTPSNGLRKAGWAPFWKRQ
                     LQNMAQGSSFIADLRRDWTRLYASPPFNFLAVAGASDQFVPPDSSLGPFDTRVQRVVP
                     GDHVSIVKPSNADAPSLTLLVATLQAGASPAPDPVAQLRLAAERPNAKVVEVVQKVET
                     TAAWMSVKNIVDVALALDGAGRRDESVAFLERYKDQDCDIKGVLAGRMKRLWYDTEKT
                     TYAERALSLYEEALRGATTADQISYLAINVAFMKLVFANDAAAARSMVAIALEHASSV
                     QDDVWRTATIAEAHLYHERVEQALAEYRRLLTLQAEPWKHHSASLQASRVATKLGRRD
                     LVEALEGIFTPGARRVNQIFVSYSHKDVEWLERLKVMMAPYLHAAESELDLWDDTRLR
                     AGQQWDEEIRRALAKAGVAVALVSADFLGSTYVMEKELPVIVNAAKGGGASLLWVYIS
                     "
BASE COUNT          256 a          512 c          513 g          242 t
ORIGIN      
        1 acgcctgact gggacatctt caccgtcggg tacgccacga cgctgctgcc ggatgtcgtc
       61 ggcatctggt ccgccgatcc cgatctgccg atcctggcca gcatgctgcg cacgcagctc
      121 ggcacgccgc cgttcagcca gtaccgatcg ctcgcgttga tcgcgcacag catgggcggg
      181 ctggtggtgc agaaggcgct ggttgacgac gcgacgctcg gacagcgagt ccgccacgtc
      241 atcctcttcg ggacgccgag caacgggctg cggaaggcgg gctgggcgcc attctggaag
      301 cgccagctgc agaacatggc gcagggcagc agcttcatcg ccgatctgcg gcgcgactgg
      361 acgcgccttt atgcgtcacc gcccttcaac ttcctcgcgg tcgcaggcgc cagcgatcag
      421 ttcgttcccc ccgattcttc actcggcccc ttcgacaccc gcgttcagcg cgtcgtcccg
      481 ggcgaccacg tcagcatcgt caaaccgtcg aacgccgatg cgccgagcct gacgctgctg
      541 gtcgcgacgc tgcaggccgg cgcctcgccc gcgcccgatc ccgtcgcgca gctccggctg
      601 gcggccgagc gtccgaacgc gaaggtcgtc gaggtggtcc agaaggtcga aaccaccgcc
      661 gcctggatgt ccgtcaagaa catcgtcgac gtggcgctgg ccctcgacgg ggccggccgc
      721 cgggacgagt ccgtggcctt cctcgagcgc tacaaggatc aggactgcga catcaagggc
      781 gtgctcgcgg gacggatgaa gcgcctctgg tacgacacgg agaagacgac gtatgcggag
      841 cgcgcgctgt cgctctacga ggaggcgctc cgcggcgcga caacggccga tcagatcagc
      901 tatctggcca tcaacgtcgc gttcatgaag ctcgtgttcg ccaatgacgc cgccgcggcc
      961 cgatccatgg tggcgatcgc actcgagcac gcctcgtcgg ttcaagacga cgtgtggcgg
     1021 acggccacga ttgccgaagc gcatctctat cacgagcgcg tcgagcaggc gctcgccgag
     1081 tatcgacggc tgctcacgct gcaggctgaa ccgtggaagc accactcggc gtcgctgcag
     1141 gcctcgcgcg tcgcgaccaa gctcggccgg cgtgatctgg tggaagctct ggaagggatc
     1201 ttcactcccg gcgccaggcg cgtgaaccag atcttcgtca gctacagcca caaggacgtc
     1261 gagtggctgg aacgcctcaa ggtcatgatg gcaccctatc tgcacgcggc agagagcgag
     1321 ctcgacctgt gggatgacac gcggctgagg gccggacagc agtgggacga ggagatcagg
     1381 cgcgcgctgg ccaaggccgg cgtggcggtc gccctcgtga gcgccgactt cctcggctcg
     1441 acctacgtca tggagaagga gttgccggtc atcgtcaacg cggcgaaggg cggcggcgcg
     1501 agcctgctgt gggtgtacat ttc
//
LOCUS       DBNGSH010000682         1791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2449943, whole genome shotgun sequence.
ACCESSION   DBNGSH010000682 DBNGSH010000000
VERSION     DBNGSH010000682.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2449943"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="AC16CY_15700"
     CDS             complement(<1..935)
                     /locus_tag="AC16CY_15700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3355293.1"
                     /translation="MSRSVSWGLTPPSRAFGSGWLSGVGSVALGGIGLLAVLCFHFPS
                     LLTVPELRTLYPVPYIRALLHLVLVGSFVLGVISVCLRRRKILGMVGISLTLVAALLG
                     GSRVPVDGELRNGPFLGLDFFLLNLMAYSAVFIPLERLFARLPEQDVFRRGWRTDLAY
                     FFLSALLVQVTTLLTMRPAMTLFSWAASAEVQRVVSAQPFVLQFAGILLLTDFVQYWI
                     HRAFHRVPVLWRFHRIHHSADVMDWLAGSRLHLVDVAVTRGLTYVPIYVLGFAEAPLY
                     AYVAFVSIQATFIHANVRFRFGPLRWVLATPQFHH"
     gene            complement(932..1660)
                     /locus_tag="AC16CY_15705"
     CDS             complement(932..1660)
                     /locus_tag="AC16CY_15705"
                     /inference="COORDINATES: protein motif:HMM:NF021998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HIR3355294.1"
                     /translation="MERCPATSSAGLPRHDAIRGLTRAVEIGSLAAALALLVANITRV
                     FAADLLLHWWAPVVVAVAAVAADFVSGLVHWTADTWFSETMPVLGRRFLRPFRVHHVN
                     PDDFLRRDVIDCNADVAMLNIPILAAALVVPVATDAGAAASLALAAFAAVSLPTNQVH
                     QWAHMPVPPAPIRWLQRHRLILSVEDHARHHDEPFVTNYCIATGWCNRMLSAMRFFPS
                     CERVITRLSGVEPRADERVWGQTP"
BASE COUNT          378 a          597 c          604 g          212 t
ORIGIN      
        1 cagtgatgga actgtggtgt cgccagcacc caccgcagcg gaccgaagcg gaagcgcacg
       61 ttggcgtgaa tgaaggtcgc ctggatgctg acgaaggcga cataggcgta gagcggcgcc
      121 tcggcgaagc cgagcacgta aatcggcacg taggtcagcc cccgcgtgac cgccacgtcc
      181 accaggtgca gccgcgaccc ggccagccag tccatgacgt ccgcagaatg gtggatccgg
      241 tgaaagcgcc acagcacggg cacacggtga aaagcgcggt gaatccagta ctgcacgaag
      301 tcggtcagca gcaggatgcc ggcgaactgc aggacgaacg gctgcgccga cactacacgt
      361 tgcacctccg ccgacgccgc ccacgaaaac agcgtcatcg ccggcctcat cgtcagcagc
      421 gtcgtcacct ggacgagcag cgcgctgaga aagaagtacg cgaggtcggt ccgccatccg
      481 cgccggaaca catcctgttc gggcaggcgc gcaaagagcc gctccagcgg aatgaacacc
      541 gccgagtacg ccatcaggtt caggagaaag aaatccaggc cgaggaacgg gccgttgcgg
      601 agctccccgt ccaccggtac gcgcgagccg ccaagcagcg ccgcgacgag cgtcaggctg
      661 atcccgacca tgcccagaat cttccggcgg cgcaggcaaa cgctgatgac gcccaacacg
      721 aacgacccca cgagcacgag gtgcagcagc gcccggatgt acggcacggg gtacagcgtc
      781 ctcagctccg gcaccgtcag cagcgagggg aagtgaaagc acagaaccgc gagaagcccg
      841 atcccgccga gcgccaccga cccgacgccg ctcaaccagc cagacccgaa cgcccgagag
      901 ggcggggtca gaccccatga cacggatcga ctcatggggt ctgaccccac actctctcgt
      961 cggctcgcgg ctccacgccg gagagccgcg tgatcacgcg ctcgcacgag gggaagaaac
     1021 gcatagccga cagcatccgg ttgcaccatc cggtcgcgat gcagtaattc gtcacgaacg
     1081 gttcgtcgtg atgccgggcg tggtcctcga cgctcaggat cagacgatgg cgctgcagcc
     1141 agcgaatcgg ggccggcgga accggcatgt gcgcccactg gtgaacctga ttcgtcggaa
     1201 gggagaccgc cgcgaacgcg gcgagagcca gcgacgccgc ggcgccggcg tcggtcgcga
     1261 cgggcaccac cagtgcggcg gcgaggatcg ggatgttgag catcgcgacg tcggcgttgc
     1321 aatcgatgac gtctcgccgc aggaagtcgt ccgggttcac gtgatgaacg cggaagggcc
     1381 gcagaaaccg gcgccccagc acgggcatcg tttcgctgaa ccaggtgtcg gccgtccagt
     1441 gcacgagccc cgagacgaag tcggcggcca cggcggccac ggccacaacg acgggcgccc
     1501 accagtgcag caggaggtct gccgcgaaga cgcgggtgat gttggcgaca agcagcgcga
     1561 gcgccgcggc gagcgagccg atttcaacgg cccgcgtcag accacgtatg gcgtcgtgtc
     1621 ggggaaggcc ggccgaagac gtcgcgggac atcgctccac gtccgaaggt atcggaggat
     1681 tccgcaaccg ggctcagagc agggagtgaa gatgtgagaa gggtctgacc ccgcttctct
     1741 cagggctgcc ggccgtccat cgacagcccg cgcagcacca acccctgccg t
//
LOCUS       DBNGSH010000683         4131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1431118, whole genome shotgun sequence.
ACCESSION   DBNGSH010000683 DBNGSH010000000
VERSION     DBNGSH010000683.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1431118"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..498
                     /locus_tag="AC16CY_15710"
     CDS             157..498
                     /locus_tag="AC16CY_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355295.1"
                     /translation="MDDVVNRFAKELADAIAAAVAENTQVEACRAKAREAGYDMRVTL
                     EAVVGFVSRNSGTAVTRITTPARLLPARKGFDLTTNDRRFLRSLRIGSEEAPTEAAAD
                     RVDSRIQSDNG"
     gene            complement(562..1305)
                     /gene="gpmA"
                     /locus_tag="AC16CY_15715"
     CDS             complement(562..1305)
                     /gene="gpmA"
                     /locus_tag="AC16CY_15715"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIR3355296.1"
                     /translation="MHRLVLLRHGESVWNKENRFTGWTDVDLSDRGREEAVEAGRLMR
                     AQNYEFDVAYTSVLKRAIRTLSIVLDEMDLMWIPEHRSWRLNERHYGALQGLNKAETA
                     AQYGEAQVKIWRRSYDIPPPPLSIDDAAHPSRDRRYASLSATDLPLGESLKDTVARFL
                     PYWHSTIAPEIKAGKRVLVAAHGNSLRALVKYLDQVSDDKIVDMNIPTGIPLVYLLDD
                     ALRPAESFYLGDQEAARRKAAAVANQAKG"
     gene            complement(1335..2639)
                     /gene="eno"
                     /locus_tag="AC16CY_15720"
     CDS             complement(1335..2639)
                     /gene="eno"
                     /locus_tag="AC16CY_15720"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR3355297.1"
                     /translation="MPITSVHAREILDSRGNPTVEADVRADGVVGRAAVPSGASTGQR
                     EALELRDGDPARYMGRGVRKAVAHVNGEIAGMLLGRELDQRAIDRALLELDGTPTKSR
                     LGANALLAVSMAAARAAAAAAGVPLYRHLSRLSEAGGTDGKYVLPAPMMNILNGGAHA
                     DSSVDLQEFMVMPLGLPTFTEALRAGTEIFHALRSILKKAGHSTGVGDEGGFAPNLKS
                     NRQALDLVMEAVGKAGLAAGRDVFLALDVASSELWDAGKYVFRKSGEPTRTSDQMVEM
                     YVDWVRQYPIISIEDGLAEGDWPGWRTLTQALGQRVQLVGDDVFVTNPEILRKGIAEG
                     VGNAILVKLNQIGTVTETLDAIAVARRANYATVISHRSGETEDSTIADLAVATSAGQI
                     KTGSASRSDRTAKYNQLLRIEEELGSGARYAGRSAIAQLIGA"
     gene            complement(2662..3927)
                     /gene="glgC"
                     /locus_tag="AC16CY_15725"
     CDS             complement(2662..3927)
                     /gene="glgC"
                     /locus_tag="AC16CY_15725"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR3355298.1"
                     /translation="MILLAGGAGERLFPLTKERAKPAVYFGGPYRIIDFTLSNCLNSG
                     LRRIFIATQYKSLSLNRHVRMGWSVVSEELGEFIEILAPQKRVSEHWYLGTADAVYQN
                     LYSVARESPSHVIVLSGDHVYKMDYSKMLHFHLEKGGDATIATIEVPISQGHRFGIVS
                     IDPNERVIGFDEKPAHPTPVPGSPDVALGSMGIYVFKADVLMRVLAEDAARPGSNHDF
                     GKDIIPALYDEAPVFSYRFYDENKKASKYWRDVGTLDAYFEANMDLCQVNPEFNLYDP
                     EWPLRTYQPQAPPAKFVFAEEGRRCGQALDSVISGGCIISGSRVSGSILGPNVRVHSF
                     CEIEQAILMPGVRVGRHARIRRAIIDRDVLVPRGASIGFNPEEDRRRHTVSEGGVVVV
                     TAEDDPLVVPISEAALRTETEADHAGSLV"
BASE COUNT          694 a         1351 c         1296 g          790 t
ORIGIN      
        1 aatgtgcctc tacaatgccc caagccttcc ctgaggctaa ccccctgcaa atgaacactt
       61 ttggtgccca atccctgcca acctccccgg tctgcgcagg ttcggattgg gagacgcccc
      121 ggatcccggc ctggtggcgg acgaggagga gcaggaatgg atgacgtcgt gaaccggttc
      181 gcaaaagagc tggccgacgc gatcgccgct gccgttgccg agaacactca ggtagaggcg
      241 tgccgcgcaa aggcgcggga agccgggtac gacatgcggg tgaccttgga agcggtagtc
      301 gggtttgtca gcaggaacag cggaacggcc gtgacccgta tcacgactcc ggctcgcctg
      361 ttgcctgcgc ggaagggctt tgacctgacg accaacgatc gccgctttct ccggtcgctg
      421 cggattgggt ccgaagaggc gcccactgaa gccgcagccg atcgggtcga ttcgcggata
      481 caaagcgaca acggctgata gtcagctggc gggttgcttg ttgctggtag ccggtagccg
      541 ggttcacacc ggctgccggc tctacccctt ggcctgattc gccacggccg ctgccttccg
      601 tcgagcggcc tcctggtctc cgaggtaaaa actctccgct ggccgcaacg cgtcatcgag
      661 caagtacacc agcggtatcc ccgttggaat gttcatgtcc acgatcttgt cgtcgctcac
      721 ctggtcgagg tacttgacca gcgcccgcag actgttgcca tgggccgcga cgagcacgcg
      781 ctttccagcc ttgatctcgg gtgcaatcgt cgagtgccag tacggcagga accgggccac
      841 cgtgtccttg agcgactcgc ccagcggcag atccgtcgcg ctgagagagg cataccggcg
      901 gtcgcgcgac gggtgcgccg cgtcgtcgat cgagaggggt ggcggcggga tgtcgtagct
      961 gcgcctccag atcttcactt gagcctcgcc gtactgcgcg gccgtctccg ccttgttcag
     1021 gccctggagc gcaccatagt gacgctcgtt cagtcgccac gaccggtgct ccggaatcca
     1081 catcaggtcc atttcgtcga ggacgattga aagcgtccgg atggcgcgct tcaggaccga
     1141 cgtatacgcg acgtcgaact cgtagttctg ggcgcgcatc agacggcctg cctcgacagc
     1201 ctcctcccgg ccccggtcgc tcagatccac atcggtccaa cccgtgaacc tgttctcctt
     1261 gttccacacg ctctcgccgt ggcgaagcag tacaagccga tgcatgtcgt ggtccccgct
     1321 ctggtctcga ggcgctaggc gccgatcaat tgagcaatgg cgctccggcc cgcgtagcgc
     1381 gccccgcttc cgagctcctc ctcgattcga agcagctggt tgtatttcgc cgttcgatcg
     1441 ctgcggctcg cggagccagt cttgatctga cccgccgacg tcgccacggc gagatcggca
     1501 atcgtgctgt cctcggtctc gccggatcga tgagagataa ccgtggcgta gttcgctcgg
     1561 cgcgcgacgg caatcgcgtc gagcgtttcg gtgaccgtgc cgatctggtt caacttgacg
     1621 aggatcgcgt tgccgactcc ttcggcaatg cccttccgaa gaatctcggg attggtcacg
     1681 aagacatcgt cgcccacgag ctgcacgcgc tgtccgagcg cctgcgtcag cgtccgccac
     1741 ccgggccagt ctccctcggc cagcccatct tcgatggaga tgatcgggta ttgccgtacc
     1801 cagtccacat acatctctac catctggtcc gaggttcgcg tgggctctcc ggacttgcgg
     1861 aaaacgtact ttccggcatc ccacagctcg ctcgacgcga cgtcgagggc gaggaacaca
     1921 tcccggcctg cggcaaggcc cgccttgcca acggcctcca tcacgaggtc gagcgcctgc
     1981 cggttcgact tcagattcgg ggcaaagccg ccttcgtcgc cgacgcccgt cgaatgcccc
     2041 gccttcttga ggatcgatcg caacgcgtgg aagatctcgg ttccggcgcg gagggcctcg
     2101 gtaaaggtcg ggagccccag cggcatgacc atgaattcct gaagatccac gctgctgtcg
     2161 gcgtgcgcac cgccgttcag gatgttcatc attggcgcgg gcagcacgta cttgccgtct
     2221 gtacccccgg cttcgctcag ccgcgagagg tgccggtaga gcggcacgcc cgccgcggcc
     2281 gccgccgcgc gcgcggccgc catggacacg gcgagcagtg cattcgcccc gaggcggctc
     2341 ttggtcgggg tgccgtcgag ttcgagcagc gcacggtcga ttgcgcgctg gtcgagttca
     2401 cggcccagaa gcattccggc aatctctccg ttgacgtgcg ccaccgcctt gcgaacgccc
     2461 ctgcccatgt agcgcgcggg atcgccgtcc ctcaactcca gcgcttccct ctgcccggtc
     2521 gacgcaccgg acggcaccgc ggcgcgcccc acgaccccgt ctgcccgcac gtcggcttcc
     2581 acggtgggat taccacgcga atcaagaatc tcccttgcgt ggacgcttgt gatcggcatg
     2641 tgtggattcc tggctccgaa ttcacaccag agacccggcg tgatcggcct ccgtttccgt
     2701 gcggagcgcc gcctcgctga ttggcacgac gagcggatcg tcttcggctg tcacgaccac
     2761 gacaccgcct tcgctgaccg tatgccggcg gcggtcttcc tccgggttga aaccgatcga
     2821 cgctccgcgg ggcaccagaa cgtcgcggtc gatgatggcg cgcctgattc gggcgtgccg
     2881 tccgacccgg acgccgggca tgaggatcgc ctgctcgatt tcgcagaagc tgtgcacgcg
     2941 gacgttgggg cccaggatgc ttccgctcac ccggctcccg gagatgatgc atccgccgga
     3001 gatgaccgaa tcgagcgcct gtccgcagcg gcgcccctct tccgcgaaca cgaatttcgc
     3061 aggcggggcc tgcggttgat acgtacgaag cggccattcc gggtcgtaca ggttgaactc
     3121 cgggttgacc tgacagaggt ccatgttcgc ctcgaaatag gcgtcgaggg tgcccacgtc
     3181 gcgccagtat ttcgaggcct tcttgttctc gtcgtagaag cgatacgaga agaccggcgc
     3241 ctcgtcgtac agcgccggga tgatgtcctt gccgaagtcg tggttgctgc cgggacgtgc
     3301 cgcgtcctcc gcgagcaccc gcatcaggac atcagccttg aagacataga ttcccatcga
     3361 cccgagcgcg acgtcgggcg agcccggcac aggcgtcggg tgcgccggtt tttcatcgaa
     3421 cccgatgacg cgttcattcg ggtcgatgct gacgatgccg aacctgtggc cctgactaat
     3481 cgggacctcg atcgtcgcaa tcgtcgcatc gccccctttc tcgagatgga agtgcagcat
     3541 cttcgagtag tccatcttgt acacgtggtc cccggacagc acgatgacgt gtgaaggcga
     3601 ttcgcgcgcg accgaataga ggttctggta cacggcgtcg gccgttccaa gataccagtg
     3661 ctcgctcacc cgcttctgcg gggcaaggat ttcaatgaac tctcccagct cctccgagac
     3721 gacgctccac cccattcgta cgtgacggtt gagcgagagg gacttgtact gcgtcgcgat
     3781 gaagatccgt cgaaggccgg agttcaggca gttgctgagc gtgaagtcga tgatccggta
     3841 cggcccgccg aagtagacgg ccggtttcgc gcgctccttg gtcagcggga agaggcgctc
     3901 gccggcgccg ccagccagta ggatcaccag gatgtcgtcg aggtccaggg cggggtgcga
     3961 atcttagcac gcgggtcgcg gcgacgatcc ccgagaggca agaaatctgc cgcaattggg
     4021 gcctttcgct ggtttgactt gggtatctgg cgtgcctatg atcagtcgat tcagattctt
     4081 ggatagccgg aatgagaggc atcatgtata tccgtcgggt actcgtcctg a
//
LOCUS       DBNGSH010000684         1569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1092335, whole genome shotgun sequence.
ACCESSION   DBNGSH010000684 DBNGSH010000000
VERSION     DBNGSH010000684.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1092335"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..504)
                     /locus_tag="AC16CY_15730"
     CDS             complement(94..504)
                     /locus_tag="AC16CY_15730"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR3355299.1"
                     /translation="MFSVTVRDHVMIAHSFKGDVFGPAQRLHGATYVVDVEFKRGSLD
                     ADGIVVDIGRATEVLRRVLADLNFRNLDEVPEFAGRNTTTEFLAHVVFGRLGEAIRRG
                     DLGPGARDVESIRVTLHESHVASASFEDRVGAAR"
     gene            complement(569..1555)
                     /locus_tag="AC16CY_15735"
     CDS             complement(569..1555)
                     /locus_tag="AC16CY_15735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3355300.1"
                     /translation="MRMSGDMEEARAFWIASPGSGEIRRERLRIPSDGDVVVQALFSG
                     ISRGTEALVFEGRVPRSEWERMRAPFQAGEFPAPLKYGYASVGRVDEGPGELRGRTVF
                     VLHPHQTRYIVPSSAVHVVPADVPPSRAVLTANLETAINGVWDGRPHVGDRISVVGAG
                     TVGCLTAWLASRIAACDVELVDVNPGRARIARALGIRFAQPHGAARDADLVIHASGSP
                     DGLALALDLAGVEATVVELSWFGDRAVPLALGGAFHSRRLTIVSSQVGRVAPAQRARW
                     DTHRRMALALRLLSDESLDVLITGESDFDELPAVMARLARAPGDTLCHRIRY"
BASE COUNT          257 a          553 c          542 g          217 t
ORIGIN      
        1 gctcgaagcc cgtctgcgat ggcgcggtcg taggcatacc ctccggttcg gccgtcgagg
       61 ctccccggca ccacgagcgc ggccgacggc acgtcagcgc gcggcaccca cgcggtcctc
      121 gaaggaggcc gacgcgacgt gcgattcgtg cagcgtgacg cgaatcgatt cgacgtcgcg
      181 cgcgcccggg ccgagatcgc cgcgccggat cgcctctccc agacggccga acacgacgtg
      241 cgcgaggaac tcggtggtcg tgtttcggcc ggcaaactcc ggaacctcgt cgagattccg
      301 gaagttcagg tcggccagca cgcggcggag cacttcggtg gcgcggccga tgtcaacgac
      361 gatgccgtcg gcgtcgagcg acccgcgctt gaactccacg tccacgacgt aggtggcgcc
      421 gtgcaggcgc tgagcggggc cgaaaacgtc gcccttgaag ctgtgggcga tcatgacgtg
      481 gtcgcggacg gtcacgctga acattcgcat ctcctggatc acgaaccacg gaggcacgga
      541 gacacggaga caccttgtcg ggccatcgtc agtaccggat ccggtggcac agcgtgtcgc
      601 cgggcgcgcg tgcgaggcgg gccatcacgg ccggaagctc gtcgaagtcg ctttcaccgg
      661 tgatgaggac gtcgagcgac tcgtcgctca ggaggcgcag cgccagcgcc atccggcggt
      721 gcgtgtccca tcgcgcccgc tgggcaggcg cgacacgccc gacctgcgac gaaacgatcg
      781 tcagccgcct ggaatgaaac gcgccgccga gcgccagcgg cacggcgcgg tcgccgaacc
      841 agctcaactc cacgacggta gcctcgacgc cggcgaggtc gagcgccagc gcgaggccgt
      901 cgggtgagcc gctggcgtgg atgacgagat ccgcgtcgcg ggcggcacca tgaggctgcg
      961 cgaaacggat gcccagcgcc cgcgcaatcc gggcgcggcc ggggttgacg tccaccagtt
     1021 cgacgtcaca ggcggcgatt ctcgacgcga gccacgcggt caggcagccg accgttcccg
     1081 cgccgacgac gctgatgcga tcgcccacgt gcgggcggcc gtcccacacg ccgttgatcg
     1141 ccgtctcgag gttcgcggtg agcactgccc tcgagggcgg tacatccgcg ggcaccacgt
     1201 gaacggccga cgagggaacg atgtagcggg tctggtgagg atggagcacg aacaccgtgc
     1261 gccctcgaag ctcgcccggc ccctcgtcta cccggccgac gctggcgtat ccgtacttga
     1321 gcggcgcggg aaactcacct gcctgaaacg gggcgcgcat ccgctcccac tcgcttcgcg
     1381 gcacgcggcc ttcgaagacg agcgcctcgg tgccgcggct gattccgctg aagagcgcct
     1441 gtacgacgac gtccccgtcc gaagggatgc ggagcctctc gcgccgtatc tcgcccgagc
     1501 ccggtgacgc aatccagaac gccctggcct cttccatgtc acccgacatt ctcatgcggt
     1561 acccgcgtc
//
LOCUS       DBNGSH010000685         5217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1092589, whole genome shotgun sequence.
ACCESSION   DBNGSH010000685 DBNGSH010000000
VERSION     DBNGSH010000685.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1092589"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1061
                     /locus_tag="AC16CY_15740"
     CDS             69..1061
                     /locus_tag="AC16CY_15740"
                     /inference="COORDINATES: protein motif:HMM:NF014899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR3355301.1"
                     /translation="MADIVLGIGSSHGPLLSTPPEQWDLRAKADRENKGHWFRGKTYD
                     FDALLAARAPGFAHEITVEKRRERFFKCRQALEALGVKFNQISPDAVVILGNDQREFF
                     NAGLTPSITVYRGSQISNVQHLNEDAPGLNIAEPANSPAEGATYPGATALADHILDSL
                     ADENFDLAQSDQTPSGAPRGGIPHAYGFFYHTILQDKTPPSVPIILNVHFPHNTPKAR
                     RCLELGRALYRAITSFTGFKRVALMASGGLTHFVIDQELDEKIMSAMQTGDEEALARI
                     PENLFKVGTAEIKNWYPVVAAMNAAGRKYHQVDYVPCYRSEAGTGNAMCFAYWE"
     gene            1079..1738
                     /locus_tag="AC16CY_15745"
     CDS             1079..1738
                     /locus_tag="AC16CY_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3355302.1"
                     /translation="MDDFLQRLSRLDSCAVSDALDKLGLAGAVSGIHRFSTDRRISGR
                     VVTVKLERDDGRPAASRHLGTTAIESSQPGDVIVVEQRTGIDAAGWGGNLSCGAQIKG
                     IAGVIIDGPGRDMDEARQYDFPVFARDHTARTARGRIVETGTNVPISVGDVQVTPGDY
                     VVADGSGVVFVAAPGIERVLEAAEAIAARERAMVAALKDGTPITQVMGRSYETMLKGG
                     G"
     gene            1738..2295
                     /locus_tag="AC16CY_15750"
     CDS             1738..2295
                     /locus_tag="AC16CY_15750"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3355303.1"
                     /translation="MVVHARAIAVAFLLLAMLAAPARAQQVTMETLLDRIQIEDLLTR
                     YYYDLSQGRAHELSEYFTEDALLDVDGTIAKGRDEIAKLYGGGPARRAEAAPRAQNHM
                     MLTNPVIEVRGNRAIARVIWTGVSNSGVGKPPTVTEQGREDSELVKTGGKWLIKRRYI
                     SSDSGLPDRFDKTFKQRNNPLEWDK"
     gene            complement(2367..2813)
                     /locus_tag="AC16CY_15755"
     CDS             complement(2367..2813)
                     /locus_tag="AC16CY_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355304.1"
                     /translation="MARALLLGVVVVLASLGPVRAVAEDAPASDFSGVYAGEGMNPDG
                     TPYTAIVEIIKLRDTYLVRWTMPDEVRVVGVGVAKAGVFAVSYFAGTPAVVVYSAETD
                     GRLEGKWTMGGAEGLLFSETLKKIPPGQRKPKPIKQGPVSHPGVPI"
     gene            complement(3069..4922)
                     /locus_tag="AC16CY_15760"
     CDS             complement(3069..4922)
                     /locus_tag="AC16CY_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3355305.1"
                     /translation="MRILMRPLAIATGCLVLLGAAWPAAADDGPTLYRQLCASCHDAG
                     LGRAPTRDVLQAMTPERVLTALESGAMLSMASGRTGVERRAIAEFVTGKTFSAPLRTT
                     PSPQAMCRATPGEFANALSGPAWNGWGVNTQNTRFQERAMAGLAAGDVPKLKVKWAFG
                     FPGELSVDSQPTMAGGRVFVGTQSGTVYALSAATGCVHWTFQAEAAVRAAVTVARIGT
                     RDVAFVGDRSANVYALDGSTGALVWKTRVDDHPFARVTGSPVFHDGRLYMGVASGEET
                     AGSTADYQCCTFRGSLVALDAATGARAWKTYTIEEPTPRAKNKVGTQLWGPSGAPIWS
                     SPAIDVQRNAIYATTGNNYSGPASERSDAFVAFDLATGRILWSRQMTPSDDWNTSCRL
                     PDQVNCTNKEAPDFDFASPPILVSLGNGRRAIVAGQKSGVVHALDPDREGQILWQQRV
                     GKGGINGGVQWGSAADGSNVYVALSDLGRIPVPNSQATVPDPEEGGGMFALRLDSGRQ
                     VWHTPPPRACRARERCSPAQSAAVSAIPGVAFSGSIDGHLRAFSTTDGAILWDVDTVR
                     SYDTVNGVTGRGGSLNVGGPAISGGTLVVNSGYVQNGAPGNVLLAFTVDGK"
     gene            complement(4984..>5217)
                     /locus_tag="AC16CY_15765"
     CDS             complement(4984..>5217)
                     /locus_tag="AC16CY_15765"
                     /inference="COORDINATES: protein motif:HMM:NF047558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR3355306.1"
                     /translation="LDELEHRRDSEYADALYMAALQDALGLRSEAFAELERAVDENSG
                     FLYSIEVDPRFDTFRDDPRFRRVRDAAGEPVIA"
BASE COUNT          931 a         1773 c         1689 g          824 t
ORIGIN      
        1 ggtatcagcg ggtgctgaca cggctcgccc cgcgcgcaaa aatcaagatc tgaccccgag
       61 gaggctgaat ggcagacatc gttcttggta ttggatcgtc gcacgggccg ctgttgtcga
      121 cgccgcccga gcagtgggac ttgcgggcga aggccgaccg cgagaacaaa gggcactggt
      181 tccgggggaa gacgtacgac ttcgacgctc ttctcgcggc gcgcgcgccc gggttcgccc
      241 acgagattac cgtcgagaag cggcgcgagc ggttcttcaa gtgccggcag gcgctcgagg
      301 cgctcggcgt gaagttcaac cagatctcgc ccgatgccgt cgtgattctc ggcaacgatc
      361 agcgggagtt cttcaacgcc gggctgacgc cgtcgatcac cgtgtaccgc ggcagccaga
      421 tcagcaacgt gcagcacctc aacgaagacg cgccgggcct caacatcgcg gagccggcca
      481 actcaccggc ggaaggcgcc acctatccag gcgccactgc cctggccgat cacatcctcg
      541 actcgctcgc cgacgagaac ttcgatctcg cgcaatcgga ccagacgccg agcggggcgc
      601 cgcgcggcgg cattccgcac gcctacgggt tcttctacca cacgatcctg caggacaaga
      661 cgcccccgag cgtgccgatc atcctgaacg tccactttcc ccacaacacg ccgaaggctc
      721 gccgctgcct cgagctcggc cgggcgctct atcgcgccat caccagcttt accggcttca
      781 agcgcgtcgc gctgatggcc tcgggcggcc tgacgcactt cgtgatcgac caggagctgg
      841 acgagaagat catgtccgcg atgcagaccg gggacgaaga ggcgctcgca cggatcccgg
      901 agaatctctt caaggtgggc acggccgaaa tcaagaactg gtacccggtc gttgcggcga
      961 tgaacgcggc cggccgcaag taccaccagg tggactacgt gccgtgctac cgatcggaag
     1021 cgggtactgg caatgccatg tgcttcgcat actgggagta acaagaaata cccgcatcat
     1081 ggatgacttt cttcagcgtc tgagccgact cgattcatgc gcggtatcgg acgcgctcga
     1141 caagctcgga ctggccggcg ccgtgtcggg cattcaccgc ttctcgaccg atcgccgcat
     1201 ctcgggccgg gtcgtcaccg tgaagctcga gcgtgacgac ggccgcccgg cggcctcgcg
     1261 ccatctgggg acgacggcca tagagtcctc gcagccgggc gatgtgatcg tggtcgagca
     1321 gcggaccggc atcgacgcgg cgggctgggg cgggaatctg tcgtgcggcg cgcagatcaa
     1381 gggcattgcc ggcgtcatca tcgacggccc cggccgcgac atggacgagg cccgccagta
     1441 cgacttcccg gtcttcgcgc gcgaccacac cgcccgcacc gcccgcgggc gcatcgtcga
     1501 gacggggacc aacgtgccga tcagcgtcgg cgacgttcag gtgacgccgg gcgactacgt
     1561 cgtcgccgac gggagcggcg tcgtcttcgt ggccgcgccg gggatcgaac gcgtgctcga
     1621 agccgcggag gccatcgcgg cgcgcgagcg cgcgatggtc gcggcgctca aggacggaac
     1681 gcccatcacc caggtcatgg gccgcagcta cgagacgatg ctgaaagggg gaggatgatg
     1741 gttgttcacg cccgtgccat agccgtggcc ttcctgctgc tggcgatgct tgcggcgccc
     1801 gcgcgggccc agcaggtcac catggagacg cttctcgacc gcatccagat cgaggatctg
     1861 ctcacgcgct actactacga cctctcgcag ggcagggccc acgagctgtc cgagtacttc
     1921 accgaagacg cgctgctcga cgttgacggc acgatcgcca agggccgtga cgagatcgcc
     1981 aagctgtatg gaggaggccc tgcccgcagg gcggaagccg cgccgcgcgc gcagaaccac
     2041 atgatgctga ccaaccccgt catcgaagtg agggggaaca gggccatcgc ccgcgtgatc
     2101 tggacgggtg tgtcgaactc cggtgtcggg aagccgccga cggtcaccga acaaggccgc
     2161 gaagactcag agctcgtcaa gaccgggggc aagtggctca tcaagcgccg ctacatcagc
     2221 tcggacagcg gcctgccgga ccggttcgac aagaccttca aacaacgcaa caacccgctc
     2281 gagtgggata agtagggtgt cacgggtccg gctgaagccg gacactacgt acgagtacgc
     2341 agtagtgtcc ggctttagcc ggacgactag atcggcactc cagggtgcga gaccggaccc
     2401 tgcttgatcg gcttcggctt gcgctggcct ggcgggatct tcttgagcgt ctccgagaac
     2461 agcaggccct cggcgcctcc catcgtccat ttcccttcga gacggccgtc ggtctcggcg
     2521 gagtagacga ccacggcggg tgttccggcg aaatagctga cggcaaacac gccagccttg
     2581 gcgacgccga cgccgacgac gcgaacctcg tccggcatcg tccacctgac aaggtacgtg
     2641 tcccgaagct tgatgatctc gacgatggcc gtgtacggcg tgccgtcggg attcattccc
     2701 tcgcccgcat acacgccgct gaagtcgctg gcaggagcat cttcggcgac cgcacgcacc
     2761 ggaccaagcg aggcgagtac gacgacgacg ccgagcagaa gcgctctggc cattgatgac
     2821 tccttactct tgctaacgct tacgagtgcc tatgcgcaca cgtatttgca ccagcaagag
     2881 ccgagccagc attggtcctt tgtgcctggt cccttggtgc tttgtccgta cttggtccgt
     2941 cgtcctttgt gcgccctgtc tccggacgga ccaaggaccg gaccaaggac tacaggacca
     3001 agatacggac caaggaccac aggaccaaga tacggaccaa gaaccaagca ctaagcacca
     3061 aggaccagtt atttcccgtc gacggtgaag gcgagaagca cgttgcccgg cgcgccgttc
     3121 tgcacgtacc ccgagttgac gacgagcgtg cccccgctga tcgccggacc gccgacgttg
     3181 agcgatccgc cgcggcccgt cacgccgttg acggtgtcgt acgatcgcac ggtgtcgacg
     3241 tcccagagaa tggcgccgtc cgtggtcgag aatgcacgaa ggtgcccatc gatcgatccc
     3301 gagaaggcca cgccgggaat ggcgctgacg gccgccgatt gcgcggggct gcaccgctct
     3361 cgcgcccggc acgctcgcgg cggaggcgtg tgccacacct gccgccccga atcgaggcgc
     3421 agcgcgaaca tgccgcctcc ttcctccggg tcgggtacgg tggcctggct gttggggacg
     3481 ggaatgcgcc cgagatccga gagcgcgacg tagacgttgg atccatccgc cgccgaaccc
     3541 cactgcacgc cgccgttgat ccctcccttg ccgacgcgct gctgccacag gatctgtcct
     3601 tctcgatccg gatcgagcgc gtgcacgacg cccgacttct ggccggcgac gatcgcgcgg
     3661 cgcccgttgc cgagcgagac gagaatgggc ggcgaggcga agtcgaagtc gggcgcctcc
     3721 ttgttcgtgc agttcacctg gtcgggcagc cggcatgacg tgttccagtc atctgacggc
     3781 gtcatctgtc gcgaccacag aatcctcccg gtggccagat cgaaggcgac gaatgcatcg
     3841 ctgcgctcgc tcgcggggcc gctgtagttg ttgcctgtcg ttgcgtaaat ggcgttgcgt
     3901 tgcacgtcga tcgcggggct ggaccagatg ggtgcacccg acggtcccca caactgcgtt
     3961 ccaaccttgt tcttcgctct cggcgtcggt tcctcgatcg tgtacgtctt ccaggccctc
     4021 gcgcccgtcg ccgcgtcgag cgccacgagg ctgccgcgga acgtgcagca ctggtagtcg
     4081 gcggtcgatc cggccgtctc ttcaccggat gcgacgccca tataaagccg gccgtcgtgg
     4141 aacaccggcg acccggtgac gcgcgcaaac gggtgatcgt caacgcgcgt cttccacacg
     4201 agcgcgccgg tggagccgtc cagcgcgtag acgttcgcgg agcggtcgcc cacgaaggcg
     4261 acgtcgcgtg tcccgatgcg cgcgacggtc acagccgcgc gcacggcggc ctcggcctga
     4321 aacgtccagt gcacgcaccc ggtcgccgcg ctgagcgcgt acaccgtgcc gctctgcgtg
     4381 cccacgaaga cgcggcctcc agccatcgtc ggctgtgagt cgacgctcaa ctcaccgggg
     4441 aatccgaacg cccacttcac cttcagcttc ggcacgtcac ctgccgcgag cccggccatc
     4501 gcgcgctcct gaaaccgggt gttctgcgtg ttgacgcccc atccgttcca cgcgggaccc
     4561 gagagggcgt tggcaaactc gccgggtgtc gcgcggcaca tcgcctgggg cgacggcgtc
     4621 gtacgaaggg gtgcactgaa ggtctttccg gtgacgaact ccgcgatcgc acggcgctcc
     4681 acgcccgtac gcccgctcgc catcgagagc atcgcgccgc tctcgagcgc cgtcagcacg
     4741 cgctcgggcg tcatggcctg caggacgtca cgcgtcggcg cccggccgag gcccgcgtcg
     4801 tggcacgagg cgcacaactg ccggtacaac gtcggaccgt cgtcggcggc agccggccat
     4861 gccgcgccaa ggagcaccag acatccggtc gcgatcgcca acggccgcat caggattcgc
     4921 atcgacaagt cctcctggaa aggcgaacat gccagggccc gacaggcgac gggcaaggga
     4981 gccctacgcg atcacaggct ccccggccgc gtctcgtacg cgccgaaacc gggggtcatc
     5041 tcggaacgtg tcaaatctgg ggtccacctc gatcgaataa agaaacccgg agttctcatc
     5101 gacggctcgt tcgagctcgg cgaacgcctc cgatcgaagt ccaagcgcat cctgcagtgc
     5161 cgccatgtac aacgcgtcag cgtactcaga gtcgcggcgg tgttcgagct cgtccag
//
LOCUS       DBNGSH010000686         1564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2959320, whole genome shotgun sequence.
ACCESSION   DBNGSH010000686 DBNGSH010000000
VERSION     DBNGSH010000686.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2959320"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="AC16CY_15770"
     CDS             complement(<1..574)
                     /locus_tag="AC16CY_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR3355307.1"
                     /translation="MTTPTKHPRTSRQRYLRFVEDYKLRRLDDQVDAAKGLKPLERPS
                     TTVEAAGSRTSQWLGGKRGEYIREYLRWLWPHRYGVGVVFGLGLVGAGLQMIEPLFMR
                     FIIDSVLLNQALDVAQRLNSLHLAGGLFVAVVVLTQLVNVTRDYRQRLLNTRVMLSLR
                     RSLFERMLRLPLPQLWEMKTGGILSRITGDV"
     gene            607..1089
                     /locus_tag="AC16CY_15775"
     CDS             607..1089
                     /locus_tag="AC16CY_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355308.1"
                     /translation="MRAILLLALAGVLTAPPARQAPIDVYDLADYRLTAQVFEPFVRA
                     SGLIADITRRDPVFADAPLFTKEIALSDDASAAASTLVARLEKHPGLAAALGTAKLTA
                     REYAKFAIALVAARLAYEFLDAGVLKRVPSGAPAENVKFVSEHRTEVLSVLETLGIRD
                     "
     gene            complement(1329..>1564)
                     /locus_tag="AC16CY_15780"
     CDS             complement(1329..>1564)
                     /locus_tag="AC16CY_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3355309.1"
                     /translation="HTTVMPFILRGVSLIGIDSALCPAGVRTEVWRRLATDMKPQRLD
                     AMSREIRLDGLPDAFATLLSGAARGRFVVDVKG"
BASE COUNT          275 a          521 c          502 g          266 t
ORIGIN      
        1 cgacgtcgcc cgtgatgcgc gacaggatgc cgcccgtctt catctcccag agctgcggga
       61 gcggcaggcg cagcatccgc tcgaacagcg atcggcggag cgacagcatg actctcgtgt
      121 tcagcaggcg ctgccggtag tccctcgtca cgttgacgag ctgtgtgagc acgacgaccg
      181 caacgaacag ccctcccgcc agatggaggc tgttcagccg ctgcgccacg tcgagcgcct
      241 ggttcaggag cacgctgtcg atgatgaagc gcatgaacag cggctcgatc atctgcaggc
      301 ccgccccgac gagcccgagg ccgaagacca cgcccactcc gtagcgatgc ggccagagcc
      361 agcgcaggta ctcgcggatg tattcgccac gcttgccgcc cagccactgt gacgttctcg
      421 agccggccgc ctcgaccgtc gtcgacggcc gctcgagcgg cttgagcccc ttcgccgcgt
      481 cgacctgatc gtcgagccgc cgcagcttgt agtcctcgac gaagcgcagg tagcgctgcc
      541 gcgacgtgcg tggatgttta gtcggcgttg tcacggtggt agcctagcaa tcgagcatat
      601 cgcaaggtgc gtgcgatcct tcttctcgcg ctggcgggcg tgctgactgc cccgccggca
      661 aggcaggctc cgattgacgt gtacgatctg gccgactacc ggctgacggc acaggtcttc
      721 gagccgttcg tgcgcgcgag cggtctcatc gccgacatca cccgacgcga tccggtgttc
      781 gccgatgcgc cgctattcac gaaagagatc gccttgtcgg acgacgcctc ggctgcggcc
      841 tcgaccctcg tggcgcggct cgagaagcac cccggcctgg ccgccgctct cgggacggcg
      901 aagctgactg cgcgggagta cgcgaagttc gccatcgcgc ttgtcgcggc ccgcctggcg
      961 tacgagttcc tggacgcggg cgtgctgaag cgggtgccgt caggagcgcc cgccgagaac
     1021 gtgaagttcg tcagcgagca tcggaccgag gtgctgtcgg tgctcgagac gctcggaata
     1081 cgcgactgat cccacagaga cttgaatcga gaatgagatc tgtcgttcac ccttctccgt
     1141 tctcggttct cggttcgtgt tcagtttcac gttcgtgtgc cgggttcact gttccgagtt
     1201 cgcgaatcgc gcccaggaac gatcaaccct gaactccgaa cacgaacccc gaaattgaac
     1261 acgaacggag aagcgagaac cgagaagcgt gaacacacga tctccgctcg acgcgagtaa
     1321 cgtggccgtc agcccttgac gtccaccacg aaccggccgc gcgcagcccc cgaaagcagg
     1381 gtggcaaagg cgtcgggcag gccgtcgagg cggatctccc gactcatggc gtcgagtcgc
     1441 tgcggcttca tgtcggtcgc caggcgccgc catacttcgg ttctcacgcc cgccggacac
     1501 agcgccgaat cgatcccgat gagcgacacg ccgcggagaa tgaatggcat gacggtcgtg
     1561 tgga
//
LOCUS       DBNGSH010000687         2553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3299799, whole genome shotgun sequence.
ACCESSION   DBNGSH010000687 DBNGSH010000000
VERSION     DBNGSH010000687.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3299799"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="AC16CY_15785"
     CDS             complement(<1..851)
                     /locus_tag="AC16CY_15785"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR3355310.1"
                     /translation="MSLALAAFVDVTIVLALTLGVSVALRRRSAALRHAVLGAGLLAA
                     AAAPALEMLVPAWEVPIAWGSTARAASSTLTLSSAAVTDGGAWTAAPAPDAVSWVDAI
                     LIAWIAGALVVLAGVITGLVRLARETRRCAVIRSGPWRELADRLSPQPVSLLESRNPS
                     LLLTWGFFRPKILLPSSAEAWSAERRAVVLAHELAHIRRGDWALQLAGETVRAIYWFN
                     PLAWIACGRLRRESEYACDDAVLMAGIEATDYATHLLDVARQAVGSRRVWTPGLAVAN
                     PSTLERRV"
     gene            complement(848..1240)
                     /locus_tag="AC16CY_15790"
     CDS             complement(848..1240)
                     /locus_tag="AC16CY_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3355311.1"
                     /translation="MAKPLHALLSRRERQIMDILFRRGRATAAEVMDELPGEPGYSTV
                     RTQLRVLEEKGYVRHDDDGVRFVYTPAVTRQSARKSALRHLIDTFFDGSPEKAMAALL
                     GGEASKLSAEQLERIAELIEKARKEGAR"
     gene            1437..>2553
                     /locus_tag="AC16CY_15795"
     CDS             1437..>2553
                     /locus_tag="AC16CY_15795"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3355312.1"
                     /translation="MNRASVVLGVVFGLVALSNAPSAQAPSTPQRQRAPSPWRFAGTQ
                     PCVNNEGGVLQCPPSPTSVAVRAGRLFDSVAGRMLTRQVVLVTGERITEVGPEGQVRI
                     PAGVPVIDLSQATVLPGLIDTHSHIYNTRRQNWTTERSTLIAIQNAQADLQAGFTAMR
                     DMGSHGNGYGDVDVRNAINWGDIDGPRLQVSGRGIAWGAKPADPSTPPNPLASIVVRS
                     VEDARAAVKEHADRGVDWIKLYPTGGYSFTPTGEARYVLTYPLPVLQALVDETHRLGK
                     KAACHAFGGDGLEFSIVAGCDSVEHGYGLTQQQLDTMVKKGLAFDPTLVRYTEPYMDD
                     NDAKNTGGKFRMIPIFDKAVAMSVATKGLKVMVGSGAD"
BASE COUNT          430 a          884 c          861 g          378 t
ORIGIN      
        1 actctccttt cgagcgttga tggattcgca acggccagtc ccggcgtcca cacgcggcgg
       61 gagccgaccg cctgccgcgc cacatccagc agatgcgtgg cgtaatcggt ggcctcgatt
      121 cccgccatga gcaccgcgtc gtcgcaggcg tattcgcttt cgcggcgcag ccgcccgcac
      181 gcgatccatg cgagaggatt gaaccagtag atggcgcgca cggtttcgcc ggcgagctgc
      241 agcgcccagt cgccccggcg gatgtgcgcg agctcgtggg cgagaacgac ggcgcgccgc
      301 tcggcactcc aggcctccgc gctcgacggg aggaggatct tcgggcggaa gaagccccac
      361 gtcagcagaa gggagggatt ccggctttca agaaggctca cgggctgcgg cgacaacctg
      421 tctgcgagct ctcgccacgg accggaacga atgacggcgc agcgccgcgt ctcgcgggca
      481 agccgcacga ggccggtgat cacgcctgcg aggacgacga gcgcgccagc gatccaggcg
      541 atcaggattg cgtccaccca ggagaccgcg tccggtgcgg gcgcggcggt ccatgcaccg
      601 ccgtcggtca cggccgctga ggacaacgtc aacgtcgagg aggctgcccg cgcggttgat
      661 ccccacgcga tcggcacctc ccaggcgggg acgagcatct cgagcgcggg agcggccgcc
      721 gcggccagca acccggcgcc aagcacggcg tgccgcaacg ccgcggagcg ccgcctcagc
      781 gcaaccgaga caccgagcgt cagggccagg acgatggtga cgtcgacgaa cgccgcgagt
      841 gcgaggctca tcgcgccccc tccttcctcg ccttctcgat cagctccgcg atgcgctcga
      901 gctgctcggc cgagagcttc gaggcctcgc cgccaagcag ggcggccatc gctttctcgg
      961 gcgaaccgtc gaagaacgtg tcgatcaggt gcctgagggc ggatttccgc gcggactgac
     1021 gcgtcaccgc cggcgtgtag acgaacctca ccccgtcgtc gtcgtgacgg acgtatccct
     1081 tttcctcgag cacccggagc tgggtgcgaa ccgtcgaata gccaggctcg ccgggcagct
     1141 cgtccatcac ttcggcggcc gtggctcggc cgcggcggaa gaggatgtcc atgatctgcc
     1201 gctcgcgtcg gctcaacagg gcgtggagcg gtttcgccat agtgttattt atctcgcacg
     1261 tgcgagataa ataacaccag tccgcggctc ctgtcaacag gcgtccacat cggccgcgtc
     1321 gcggcgccga cgcgtctgta taatccgcag gtccggctaa agccggacac tacgtacgac
     1381 acggtacggg cagtcgtcgt gtccggcttc ggccggaccg tgatcatgga gggaatatga
     1441 atcgcgcgtc agtcgtgctg ggtgtcgttt tcggcctggt tgcgctttcg aacgctccgt
     1501 cggcgcaggc gccctcgacg ccacagcgtc agcgcgcgcc cagtccgtgg cggtttgccg
     1561 gcacccagcc ctgcgtgaac aacgaggggg gcgtcctgca gtgcccgccg tcgccgacat
     1621 cagtcgccgt ccgtgccggc cggttgttcg acagcgtcgc cggccggatg ctgaccaggc
     1681 aggtcgtgct cgtcaccggc gagcggatca ccgaggtcgg ccccgagggc caggtgcgga
     1741 ttccagcggg cgtgccggtg atcgatctca gccaggcgac ggttctgccc ggcctcatcg
     1801 acacccactc gcacatctac aacacccgcc ggcagaactg gacgaccgag cgatcgacgc
     1861 tgatcgccat ccagaatgcg caggcggatc tgcaggcagg cttcacggcc atgcgcgaca
     1921 tgggctccca cggcaacggc tacggcgacg tcgatgtccg caacgcgatc aactggggcg
     1981 acatcgacgg cccgagactg caggtgtcgg gacgcggaat cgcctggggc gccaagcccg
     2041 ccgatccctc gactccgccc aacccgctcg ccagcatcgt cgtccggtcg gtcgaggacg
     2101 cgcgcgccgc ggtcaaggag cacgcggacc ggggcgtgga ctggatcaag ctctacccga
     2161 ccggcggcta ctccttcacg ccgacgggcg aagcgcggta cgtgctcacg tatcccctgc
     2221 cggtgctgca ggcgctcgtg gacgagaccc accgcctcgg caagaaggcg gcgtgtcatg
     2281 ccttcggcgg cgacggcctc gagttcagca tcgtcgctgg atgtgattcc gtcgagcacg
     2341 ggtacggcct gacccagcag cagctcgaca cgatggtcaa gaaggggctc gccttcgacc
     2401 cgacgctcgt gcgctacacc gagccctaca tggacgacaa cgacgcgaag aacaccggcg
     2461 gcaagttccg gatgatcccg atcttcgaca aggccgttgc catgtcggtc gccacgaagg
     2521 ggctcaaggt catggtcggc agcggcgccg acg
//
LOCUS       DBNGSH010000688         4588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_73296, whole genome shotgun sequence.
ACCESSION   DBNGSH010000688 DBNGSH010000000
VERSION     DBNGSH010000688.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_73296"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="AC16CY_15800"
     CDS             complement(<1..629)
                     /locus_tag="AC16CY_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3355313.1"
                     /translation="MRRIVTVDTCNNFIDGKWVPSRSGRTFENRNPADHRDVIGVFQD
                     STAEDAESAIAAAQRAYPSWRLVPAPRRAEMLLAAARLLVERKEAFARDMTREMGKVL
                     EETRGDVQEAIDMTSFMAGEGRRLYGQTVPSELRDKFAMSVRQPLGVCSIITPWNFPM
                     AIPSWKISPALVCGNTVVFKPATLTPLSAVNFVKVLAEVGIPPGVVNLVT"
     gene            954..1331
                     /locus_tag="AC16CY_15805"
     CDS             954..1331
                     /locus_tag="AC16CY_15805"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3355314.1"
                     /translation="MNDKARNIAYPAQKVVVVNGNTDVLGILESVLDAGRYDMVFVEP
                     GDRAYSQIKKVLPNLVILCTRIERLDGFQVLSMLKLDPETRDIPVLTYTTEHEGQDFD
                     AVLSRRTDEDETVLRSQSALRMN"
     gene            1363..1701
                     /locus_tag="AC16CY_15810"
     CDS             1363..1701
                     /locus_tag="AC16CY_15810"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3355315.1"
                     /translation="MSEEKREGSRVAMIGRLEGEVMVFQPMVIKEVSIGGAMVETRFP
                     LHLNSLHDLRLELGPRSVVVKGRVVHSQIADVDEDVVIYWTGVEFIEPSEHASSAIAD
                     FLKSVKTERH"
     gene            complement(1719..2123)
                     /locus_tag="AC16CY_15815"
     CDS             complement(1719..2123)
                     /locus_tag="AC16CY_15815"
                     /EC_number="4.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF017868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061929 -
                     gamma-glutamylaminecyclotransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIR3355316.1"
                     /translation="MHKLVFFYGTLMTPFNRTGLLGIDQHLVYRGRGTIAAALFDLGI
                     YPAAVPDPDGLVWGEVYEMTHPVAVLRALDETEAYRPSEPESSLYTRRLTPVTMEDGE
                     IVRAHAYFYNAPLGRAERIASGDYLQHLNVRQ"
     gene            complement(2219..3238)
                     /locus_tag="AC16CY_15820"
     CDS             complement(2219..3238)
                     /locus_tag="AC16CY_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355317.1"
                     /translation="MSKRTRLFLSVAAGILVVGLGTGLLASFMGFQALTLAGASTPEF
                     SYVPRDVQFLAFANVRNVMDSELRRKLTELKPDVENGADQLKQHTGIDVRTDIDSVLA
                     TWLSPPGGEGGPPLVIARGRFDDAQIESLITAQGGRAEDYKGKRLLLHEQEMFAVVFL
                     EPGLAAIGSPASVRTAIDTQMSGNDVNGNDELMRLTGDINDGSVWAVARFDALVRPLP
                     ADVATQLPAISWFSAKGFIDSGVQGQLRVEARDEESARNLQDVVRGFVALARLQVSQQ
                     AGLAQVLDSLLLSGEGKTVSLSFSVPSELIVDTLGAAIRAGGQKPSTEPFLAPAPEPA
                     PAPAL"
     gene            complement(3295..4128)
                     /locus_tag="AC16CY_15825"
     CDS             complement(3295..4128)
                     /locus_tag="AC16CY_15825"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355318.1"
                     /translation="MTTDRRSQSFLTGAWRVFELSLGEMLWSRRTIFMALVVGCPVVV
                     ALVARIVQTAGLSLLRVNGVQIDAMNMFGVMIWTLFMWFIVPVLGAFYGTSLIADEVD
                     DKTITYLFTRPVRRGAVLFGKFIAYLVCTTFVVLPSVMIVYFLIVPIGQIAATFTSLL
                     IDLGVIGLGLIAYGSLFAFVGTALKRPLVVGLVFAFGWEQAALFLPGYVKRFTLAYYL
                     QTMVPHAMPSGGVVSALQGAMFKDPPSVGMSLLCLSLAIVVSLAAAVRVVERREYVLE
                     Q"
     gene            complement(4125..>4588)
                     /locus_tag="AC16CY_15830"
     CDS             complement(4125..>4588)
                     /locus_tag="AC16CY_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3355319.1"
                     /translation="LILDEPLSGMDPLMRRKTIRMIKDWGRAGKSVLVSSHILHEVES
                     MTPNILLINNGRILAEGNVHQIRDLIDEHPHTVFVRTDDPKRLARALLVEDGIISLRF
                     EQQAVIAETAHPDAFYARLTDMAASGEAGVIDEITSPDDNLQAVFKYLVKT"
BASE COUNT          888 a         1476 c         1460 g          764 t
ORIGIN      
        1 gtcacgaggt tgaccacgcc tggaggaatg ccaacctccg ccagtacttt cacgaagttg
       61 accgcggaga gcggcgtcag cgtcgccggc ttgaagacca cggtgttgcc gcacaccaga
      121 gcgggtgaaa tcttccacga cggaatggcc atcggaaaat tccacggcgt gatgatcgag
      181 cagacgccga gcggctggcg gaccgacatc gcgaacttgt ctcgaagctc ggagggcacc
      241 gtttgtccgt acagcctgcg tccctctccc gccatgaagg acgtcatgtc gatcgcctct
      301 tgtacgtccc cgcgggtctc ttcgagcacc ttgcccatct cgcgcgtcat gtcgcgcgca
      361 aaggcctcct tgcgctcgac cagcaggcgc gccgccgcca gcaacatctc ggcccggcgc
      421 ggcgcgggta cgagcctcca ggagggatac gcgcgctgcg cggccgcgat cgccgattca
      481 gcgtcctccg cggtcgagtc ctggaacaca ccgatcacgt cgcggtggtc cgcgggattc
      541 cggttctcga acgtcctccc cgatcgcgat ggaacccact ttccgtctat gaagttgttg
      601 caggtgtcca cggtgacgat cctccgcatc caccccacgg cgcacgcgtg cgccgcgagg
      661 accccggtct acagactgcc gcaatctgtc acaacggaca atacgggcag ctcgaccgca
      721 aaaaacgggg agaaattcct gcgcaatgtt cagtaggcct cgtgagtgtg caagaggctt
      781 taactagccg ccgctgaagc agttatcctc cacggccagg ctgcgggccg ccggagacgg
      841 gcctgtcgtc agggacggat tgcacgataa gacaatggaa cgtggtgatg gagcactaca
      901 cgcccatggc acggatcctg tatcaggaat ggtcagaagg ttcggatacc gagatgaatg
      961 acaaggcccg caatatcgcc tacccggcac agaaggttgt cgtcgtgaac ggcaacaccg
     1021 acgtgctcgg cattctcgaa tcggtactcg atgctggccg ttacgacatg gtcttcgtcg
     1081 agccgggcga ccgcgcgtac tcgcagatca agaaagtgct gcccaacctg gtcatcctct
     1141 gcacgcggat cgagcgcctt gacggattcc aggtcctgtc gatgttgaag ctggacccgg
     1201 agacccgcga cattccggtg ctgacctaca cgaccgagca cgagggacag gatttcgacg
     1261 ccgtgctctc acgtcggacc gacgaggacg agacggtgct gagaagccag tcagcactcc
     1321 ggatgaacta accagctcgg cctagtggta tattccccgc caatgtcaga ggagaagcgc
     1381 gaaggctctc gagtggccat gatcggccgc ctcgagggcg aggtcatggt gtttcagccg
     1441 atggtgatca aagaggtcag tatcggaggc gccatggtcg agacccgctt cccgctccac
     1501 ctcaattcac ttcacgacct gaggctcgag ctgggacccc gttccgtcgt cgtcaaaggc
     1561 cgcgtggtgc actcgcaaat cgcggacgtg gacgaggacg tcgtcatcta ctggacgggc
     1621 gtcgagttca tcgagccatc ggaacacgcc tcgagcgcga tcgccgactt cctcaagtcg
     1681 gtcaagaccg agcgccactg acctggccga gtacggcctt actggcggac gttgaggtgc
     1741 tgcagatagt cgccggacgc aatccgctcc gcgcggccga gaggcgcgtt gtaaaagtac
     1801 gcgtgagccc ggacgatttc accgtcttcc atcgtgaccg gcgtcagtcg ccgcgtgtag
     1861 aggctgcttt cgggctcgct cgggcggtag gcctcggtct cgtccagggc ccttaacacc
     1921 gcgacggggt gcgtcatctc gtacacctcc ccccagacga gtccgtcggg gtccgggacg
     1981 gccgcggggt agatgccaag atcgaacagg gcggccgcga tggtgccgcg tcctcggtac
     2041 acgaggtgct ggtcaatccc tagaagcccc gtgcgattga agggcgtcat cagggtgccg
     2101 tagaagaaca cgagcttgtg catctcgatg agcggcctcg gggggcgaca aagcccggtg
     2161 ggcggacgcc accgccatcc ccactagtca gacactacga gccggatcgg gatgctattt
     2221 acagggccgg cgcgggggca ggctcggggg ccggcgccag gaagggctcg gtactcggct
     2281 tctggccgcc cgcgcgaatg gcggcgccca gggtgtcgac gatcagctcc gatgggaccg
     2341 agaacgaaag cgagaccgtc ttgccttcgc cggaaagcag gaggctatcg aggacctgcg
     2401 ccagcccggc ctgctggctg acctgcagtc gagcgagcgc cacgaacccg cgaacgacgt
     2461 cctgaagatt gcgggccgat tcctcgtccc ttgcctctac gcgaagctgt ccctgcacgc
     2521 cgctgtcgat gaagcccttc gcggagaacc agctgatggc cgggagctgc gtggcgacgt
     2581 cggccgggag cggccggacg agcgcatcga accgcgcgac cgcccagacg ctgccgtcgt
     2641 tgatatcgcc cgtgaggcgc atcaattcgt cgttgccgtt gacatcgttg ccgctcatct
     2701 gcgtatcgat ggccgtccgc accgaggccg gggatccgat cgcggcgagc cctggctcca
     2761 ggaacacgac ggcgaacatt tcctgttcgt gcaggaggag cctcttgccc ttgtagtcct
     2821 cggccctgcc gccctgcgcc gtgatcaggc tctcgatctg cgcgtcgtcg aagcgccccc
     2881 tggcgatgac gagcgggggc ccaccctcgc cgcccggagg gctcagccac gtcgcgagaa
     2941 ccgagtcgat atccgtccgg acgtcgatgc cggtgtgctg cttcaactgg tcggcgccgt
     3001 tttcaacgtc gggcttgagc tcggtcaact tgcggcgcag ctcggagtcc atcacgttgc
     3061 gcacattggc aaaggccagg aactggacgt ccctgggcac ataggagaac tcgggggtac
     3121 ttgcacctgc cagcgtcagg gcctgaaaac ccatgaacga ggccagcagg ccggtgccaa
     3181 gaccgacgac gaggatgccg gcggcgaccg acaggaaaag ccgagtgcgc ttgctcattt
     3241 acaccgcccc ctggctcata tacggtgggc cgggcgccca ggttcctgcc cctattattg
     3301 ctccagaaca tactcgcgac gctcgaccac acgaaccgcg gcggccaacg ataccactat
     3361 ggccaacgag aggcacagca gactcatccc cactgagggg ggatccttga acatggcgcc
     3421 ctggagggcg gacaccacgc ctcccgacgg catcgcgtgg gggaccatcg tctggaggta
     3481 gtaggccaga gtgaaccgtt tcacgtatcc gggaagaaac agcgcggcct gctcccagcc
     3541 gaacgcgaac accagcccga ccacgagcgg gcgcttgagc gcggtgccga caaaggcgaa
     3601 cagcgagccg taggcgatca ggccgaggcc gatcacgccc agatcgatga gcagcgaggt
     3661 gaacgtggcc gcgatctgtc caatcggaac gatcaggaag tagacgatca tcaccgacgg
     3721 cagcacgacg aacgtcgtgc acacgagata ggcgatgaac ttgccgaaca gcacggcccc
     3781 gcggcgcact ggccgcgtga acaagtaggt aatcgtcttg tcgtctactt cgtctgcgat
     3841 cagggaggtg ccgtagaagg ctcccagcac cgggacgatg aaccacatga acagggtcca
     3901 gatcatcacg ccgaacatgt tcatggcgtc gatctgcacg ccgttcaccc tgagcagcga
     3961 caagcccgcc gtctgcacga tccgtgcaac cagcgcaacg acgacgggac aaccgacgac
     4021 gagcgccatg aagatggtcc gccgcgacca gagcatctcg ccgagcgaca gctcgaagac
     4081 gcgccaggcg cccgtcagaa acgactgcga gcggcggtcc gtggtcatgt cttcaccagg
     4141 tacttgaaaa cggcctgcag attgtcgtcg ggagacgtga tctcgtcgat cacgccggcc
     4201 tcgccggacg cggccatgtc ggtcaggcgc gcgtagaacg catcggggtg cgcggtctcc
     4261 gcaatcacgg cctgctgctc gaaccgcaga ctgataatcc cgtcctcgac cagcagggcg
     4321 cgcgccagtc gcttcggatc gtcagtgcgc acgaacacgg tgtgcggatg ttcgtcgatg
     4381 aggtctctga tctggtggac gttgccttcg gccaggatcc ggccgttgtt gatcagaagg
     4441 atgttcggcg tcatcgactc gacctcgtgc aggatgtggc tcgacaccag cacgctcttc
     4501 cccgcccggc cccagtcctt gatcatgcga atcgtcttgc ggcgcatgag cgggtccatg
     4561 ccgctcagcg gctcgtccag gatgagca
//
LOCUS       DBNGSH010000689         7719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3469709, whole genome shotgun sequence.
ACCESSION   DBNGSH010000689 DBNGSH010000000
VERSION     DBNGSH010000689.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3469709"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /gene="nrfD"
                     /locus_tag="AC16CY_15835"
     CDS             <1..424
                     /gene="nrfD"
                     /locus_tag="AC16CY_15835"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3355320.1"
                     /translation="NEAFFAWYSASPHEAAMMANRMGGPYAFFYWSLIAINAVMIQLF
                     WFRSVRENIPLLFVLSLFVNVGMWLERFIIIVTSLHRDYLPSSWDMYAPTRWDFGMFA
                     GTIGLFLALFFLFIRLLPAIAIFEMRTIVPEAKVKHHG"
     gene            429..974
                     /locus_tag="AC16CY_15840"
     CDS             429..974
                     /locus_tag="AC16CY_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HIR3355321.1"
                     /translation="MEHTQSDTLYGLMAEFDNPTSLVEAATKARLAGYRKIDAYTPIP
                     IEELTEALDLPRTKVPLITLIGGIVGGLSGYGLAYWTSAVAYQMNVGDRPLNSWPQFI
                     PVTFEMTVLGAALSAFVGMWALNRLPQPYHPVFNVAAFGRASTDRFFLVIEQADPRFE
                     RHGTWKFLEGLRPVGVSEVAP"
     gene            964..1536
                     /locus_tag="AC16CY_15845"
     CDS             964..1536
                     /locus_tag="AC16CY_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3355322.1"
                     /translation="MRHKVLTIVAACFMAACRQDMHDAPRYEPLEESAFFAGGGSART
                     LVANTVPRGHLREDELLYTGKIGGQLANMFPMPVTADVMARGQERFNVFCSPCHGRTG
                     EGNGMIVQRGFRQPPSYHEQRLRDAPAGYFFDVMTNGFGAMQDYAAQVPVADRWAIAA
                     YIRALQLSRAATVDDVPSDRREELNRQENK"
     gene            1533..2759
                     /locus_tag="AC16CY_15850"
     CDS             1533..2759
                     /locus_tag="AC16CY_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355323.1"
                     /translation="MTVDTTYSPPAADIDHLKSRALIAGVIGLVGCGIGVLVDRDHFF
                     RSWLVAYMLFFGIALGSLALIMVQHLSGGAWGVFRRVFEASSRTLPLLMVLFVPVLIG
                     MGTLYPWTHADHVAADEVMTKKAPYLNTTFFVVRAVVYFAGFWLLATLLNKWSRQQDE
                     GNVGANVRLQRLSGAGLVFYALAVTFAGVDWVMSLNPHWYSTIFGFLMIGGQGLAALA
                     FTILVSAFLFRRNPMADLLKPSHFHDLGKLMFAFVMLWAYFNFSQYMLTYAANLIEEV
                     PYMITRTTHGWQYLALFLVIFHFAVPWLLLLSRDLKRNPGRLVVVAVWMLFVRFADLY
                     MMVSPEFDASGGSNLHLLAGEHASHFFVHWLDLAAPLAIGGLWLWMFFTELRRRPLLA
                     TGDPYLRAALESGGGH"
     gene            2759..3268
                     /locus_tag="AC16CY_15855"
     CDS             2759..3268
                     /locus_tag="AC16CY_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355324.1"
                     /translation="MAHDPHAAHASADDEYLNPPSGSGHEHTDADVRQIIQFAVWLAV
                     SALVVHVLMWLTFGLFERTTVNKGPAEYPLAIEQGPRLPSGPRLQPIPANEIYQFRLK
                     ETAALDSYAWTDKATGKVQIPISEAMRLTVERGLPARTAEGASQAGEASGLMPTDASA
                     GRAMERRRQ"
     gene            3265..4134
                     /locus_tag="AC16CY_15860"
     CDS             3265..4134
                     /locus_tag="AC16CY_15860"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3355325.1"
                     /translation="MKRLLLSLGLVTFAFCLAGSRLSAQQQPGMPGSMGMTGGIVASN
                     VPPKFKDVTFAQRLGEQLPLDARFTDEEGRAVTLGNYFGRKPVVLAFVYYQCPMLCPL
                     VMNGISSALTVVPFEVGKDFDVVLISFDARDTSAAANAKKRAHLLNWSASDTAAGWHF
                     LSGEDSEIRRVATAAGFTYQWDEQTQQFAHVSGLLVATPDGRLSRYFYGVEYSPRDLR
                     MALVDSGQGRLGSVVDELLLYCFHYDPSSGQYGAVFMNIMRLGGVLTVFLIAGYVVLM
                     RWRETRQIAERHA"
     gene            4137..5138
                     /gene="coxB"
                     /locus_tag="AC16CY_15865"
     CDS             4137..5138
                     /gene="coxB"
                     /locus_tag="AC16CY_15865"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3355326.1"
                     /translation="MFPRISLFPAQASTMAAQVDLLYLFIVGVTAFFGILTTIVVIVF
                     AVKYRTNDPLKVGDRIHGSIPLELAWSIIPFIISIVIFWWSAVVFFDLQRPPDQTLEI
                     YATGKRWMWKFQHLDGQNEINELHVPMGRPIKVTFTSEDVLHSLFFPAFRTKADAIPG
                     RYSSVWFTPTKLGEYHLFCAEYCGTKHSGMIGRVVVMEPAAYQAWLSGSVPGMSLAAR
                     GERLFSELACNTCHLDDGRGRGPSLVNKFGASEQLASGGAVPVDEAYVRESILRPQSK
                     IVAGYQPLMPTFQGLVSEENVMALVEYVKSLRATAQAAAPAAAHAESTAPGAPQESR"
     gene            5142..6758
                     /locus_tag="AC16CY_15870"
     CDS             5142..6758
                     /locus_tag="AC16CY_15870"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR3355327.1"
                     /translation="MDTTVTAPRVTYLNHEHTLASWLLTKDHKRIGILYMIVVTFAFF
                     LGGIFASLIRLELTTPAGDLLSADTYNKMFTMHGVAMVFFVLIPAVPAILGNFVLPLM
                     LGAKDVAFPKLNLISWYLFTIGFLFTVYALVAGGVDTGWTFYTPYSSRASNTNVMATG
                     LGVFITGFSSILTGLNFMVTIHRMRAPGLTWFRLPLFVWAVYSTALVMILGTPVIAIT
                     VLALAIERFLHFGIFDAALGGDPVLFQHMFWFYSHPAVYIMILPPMGAISEVIAAFSR
                     KSIFGYAFVAFSSLAIAIFGFLVWAHHMFVSGISVYSALVFSFLTYAVAIPSAVKTFN
                     WAATMYRGAIWLKAPMIWAIGFLGLFLVGGLTGLFTAALGVDVHLHDTYFVVAHFHYI
                     MVGGAIMGYFAGLHFWWPKITGRMYSDIWAKVAAIIVFTGFNLTFGPQFIVGYLGMPR
                     RYYAYPEEFQVLNVLSSAGASILGLGYTLIAIYLVASLFKGERVGANPWHATGLEWTT
                     QSPPLPHNFEKQPVVTEDAYEYTKREIKVV"
     gene            6751..7440
                     /locus_tag="AC16CY_15875"
     CDS             6751..7440
                     /locus_tag="AC16CY_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HIR3355328.1"
                     /translation="MSDALAAHGAHGHAHHPAQQHHFDTLDQQHEAATLGMWLFLITE
                     VLFFGGLFLAYMLYRVWYPAAWSEASLELNVVLGGVNTAVLIASSLTMAMAVRGAQTG
                     LRRATMGWLVLTMVFGLTFLVIKYFEYAEKIEHHHVPGPTFAFPGPNAGPAEIYFSLY
                     FLMTGLHATHMVIGFGILSVILWMAYKNRFSPEWYTPVEISGLYWHFVDIVWIFLFPL
                     LYLVDRAHHFA"
     gene            7448..>7719
                     /locus_tag="AC16CY_15880"
     CDS             7448..>7719
                     /locus_tag="AC16CY_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR3355329.1"
                     /translation="MAGHISPKGTYYAIFGILMVLTLVTVYVAYQPLGAANFPVAIGI
                     AITKATLVILFFMHAKYSSRLTKLFVGTAFFFLAILLGLSLTDYMSR"
BASE COUNT         1206 a         2569 c         2415 g         1529 t
ORIGIN      
        1 gaacgaggcc ttcttcgcct ggtacagcgc gagcccgcac gaagcggcca tgatggccaa
       61 ccgcatgggc gggccctacg cgttcttcta ctggtcgctc atcgccatca acgcggtgat
      121 gatccagttg ttctggttcc ggagcgttcg cgagaacatc ccactgctct tcgtgctctc
      181 gctcttcgtg aacgtcggca tgtggctcga gcgcttcatc atcatcgtca cgagcctgca
      241 tcgcgactac ctgccctcgt cctgggacat gtacgccccg acgcggtggg acttcggcat
      301 gtttgccggc acgatcggac tgttcctggc cctcttcttc ctcttcatcc gcctcctgcc
      361 agcgatcgcc atcttcgaga tgcggacgat cgttcccgag gcgaaagtga agcaccacgg
      421 gtgatgtaat ggaacacacc cagagcgaca cgctgtacgg gttgatggcc gagttcgaca
      481 acccgacctc gctcgtcgag gccgcgacga aggcacgcct ggcgggctac cgcaagatcg
      541 acgcctacac cccgatcccg atcgaggagt tgaccgaggc gctcgatctg ccgcggacga
      601 aggttccgct gatcacgctg attggcggca ttgtcggcgg gctttccgga tacggcctcg
      661 cgtactggac gtcggcagtc gcctatcaaa tgaacgtcgg cgatcggccg ctcaacagct
      721 ggccacagtt catcccggtg acgttcgaga tgacggtcct cggcgcggcg ctgagcgcct
      781 tcgtcgggat gtgggcgctg aaccggctcc cgcagccgta tcacccggtg ttcaacgtcg
      841 ccgcgttcgg ccgcgcctcc acggatcgat tcttcctggt catcgaacag gcagacccgc
      901 gattcgagcg gcacgggacc tggaagttcc tcgaggggct gcgcccggta ggagtatccg
      961 aagttgcgcc ataaggttct gacgatcgtg gcggcgtgct tcatggcggc ctgtcgtcag
     1021 gacatgcacg acgcgcccag gtacgagccg ctcgaagaga gcgccttctt tgccggcggc
     1081 ggctccgcgc gcacgctcgt ggccaacacc gttccccgcg gccacctccg tgaggacgag
     1141 ctgctttaca ccggcaagat cggcggacag ctcgccaaca tgttcccgat gccggtcacg
     1201 gccgacgtca tggcgagagg ccaggagcgc ttcaacgtgt tctgctcgcc ctgccacggg
     1261 cggaccggcg agggcaacgg catgatcgtg cagcgcggct tccgccagcc gccgtcgtac
     1321 cacgagcagc ggctgcgcga cgctccggcg ggctacttct tcgacgtgat gacaaacggc
     1381 ttcggggcga tgcaggacta cgctgcgcag gtgccggttg ccgaccgatg ggcgatcgcg
     1441 gcctacatcc gtgcgctgca gttgagccgc gccgccaccg tggacgacgt gccgtcggac
     1501 cggcgcgaag agctgaaccg gcaggagaac aagtgaccgt ggacacgacg tattcgccgc
     1561 ctgccgccga catcgaccac ctcaagtcgc gcgcgctgat tgccggcgtg atcggtctcg
     1621 tcggctgcgg catcggtgtc ctggtcgacc gggaccactt cttccgctcg tggctcgtgg
     1681 cgtacatgct gttcttcggg atcgccctgg gttcgctggc gctcatcatg gttcagcatc
     1741 tctcgggcgg cgcctggggc gtgttcaggc gggtcttcga ggcctcgagc cggacgctgc
     1801 cgctgctcat ggtcctgttc gtccccgtgc tgatcggcat ggggacgctc tatccctgga
     1861 ctcatgccga ccacgtcgcc gccgacgagg tcatgacgaa gaaggcgccc tatctgaaca
     1921 cgacgttctt cgtcgtgcgc gccgtcgtct acttcgcggg cttctggctt ctcgccacgc
     1981 tgctcaacaa gtggtcgcgg cagcaggacg agggcaacgt cggcgccaac gtccgactcc
     2041 agcgcctgag cggcgcggga ctggtcttct acgcgctggc cgtgacgttt gccggcgtcg
     2101 actgggtcat gtcgttgaac ccgcactggt actcgacgat cttcgggttc ctgatgatcg
     2161 gcggacaagg cctggccgcg ctcgccttca cgatcctcgt ctcggccttc ctgttccgcc
     2221 gcaacccgat ggcggacctg ctcaagccct ctcatttcca cgatctcggc aagctgatgt
     2281 tcgcgttcgt gatgctgtgg gcgtatttca acttctcgca gtacatgctc acctacgcgg
     2341 ccaacctgat cgaggaagtc ccgtacatga tcacgcggac gactcacggg tggcagtacc
     2401 tggcgctctt tctcgtgatc ttccacttcg ccgtgccctg gctgctgctg ctctcgcggg
     2461 atctgaagcg caatccgggc cggctcgtcg tcgtggcggt gtggatgctt ttcgtgcggt
     2521 ttgccgacct ctacatgatg gtctcgcctg aattcgacgc cagcgggggc agcaaccttc
     2581 atctgctggc cggcgagcat gccagccact tcttcgtgca ctggctcgac ctggcggcac
     2641 cgcttgcgat tggcggcctg tggctctgga tgttcttcac ggagctgcgg cggcggccgc
     2701 tgctggcaac cggcgatccc tacctgcgcg cggcgctcga gagcggggga gggcactgat
     2761 ggcgcacgat cctcacgcgg cgcacgcctc ggccgacgac gagtatctga accctccatc
     2821 ggggtcgggt cacgagcaca ccgacgccga cgtgcggcag attatccagt tcgccgtctg
     2881 gctggccgtg tcggccctcg tcgtccacgt cttgatgtgg ctgaccttcg ggttgttcga
     2941 gaggacgacc gtcaacaagg gcccggccga gtacccgctc gccatcgaac aggggccgcg
     3001 cctcccgtca gggccgcgtc tgcagccgat tccggccaac gagatctatc agttccgtct
     3061 caaggaaact gccgctctcg acagctacgc gtggactgac aaggcgacag gaaaggtcca
     3121 gattccgatt tcggaagcca tgcggctgac ggtcgagcgc ggcctgcccg cccgaacggc
     3181 cgaaggcgcc agccaggccg gtgaggcgtc cggcctgatg ccgaccgatg cgagcgccgg
     3241 ccgtgccatg gaaagaagaa ggcaatgaaa cggttgcttc tgtcgcttgg ccttgtgact
     3301 tttgcctttt gccttgcagg gtcgcgcctg agcgcccagc agcagccggg catgcccggc
     3361 tccatgggga tgaccggcgg catcgtggcg tcgaacgtcc cgccgaagtt caaggacgtg
     3421 acgtttgccc agcggctcgg cgagcagctc ccgctcgacg cgcgtttcac ggacgaagag
     3481 gggcgcgccg tcacgctggg caactacttc ggccgcaagc ccgtcgtgct ggcgttcgtg
     3541 tactaccagt gcccgatgct ctgcccgctg gtcatgaacg ggatctcctc ggccctcacc
     3601 gtcgtcccct tcgaggtcgg caaggacttc gacgtcgtcc tcatcagttt cgacgcgcgc
     3661 gacacgtctg cggccgcgaa tgccaagaag cgcgcgcacc tgctgaactg gtccgcctcc
     3721 gacaccgcgg ccgggtggca tttcctgagc ggcgaggaca gcgagatccg ccgcgtcgca
     3781 acggcggccg gcttcaccta ccagtgggac gagcagacgc agcagttcgc gcacgtgagc
     3841 ggcctgctcg tggcgactcc ggacgggcgg ctgtcgcgct acttctacgg cgtggaatat
     3901 tcgcctcgcg acctgcgcat ggcgctcgtg gactcgggac agggacgcct cggatcggtc
     3961 gttgacgagc tgctgctcta ctgtttccac tacgacccgt cgagcggcca gtacggcgcc
     4021 gtgttcatga acatcatgcg cctgggtggt gtgctgaccg ttttcctgat cgccggttac
     4081 gtcgtgctga tgcgatggcg tgaaacgcgc cagatcgcgg aaaggcacgc ctgagcatgt
     4141 tcccccggat ttccctcttt cccgcgcagg cgtcgacgat ggcggcgcaa gtcgacctgc
     4201 tgtacctgtt catcgtcggc gtcacggcct tcttcggcat tctcacgacc atcgtcgtga
     4261 tcgttttcgc cgtcaagtac cgcacgaacg atcctctcaa ggtcggcgac cgcatccacg
     4321 ggtccatccc gctcgagctg gcctggtcga tcatcccgtt catcatcagc atcgtcatct
     4381 tctggtggag cgcggtcgtg ttcttcgatc ttcagcgacc gcccgaccag acgctcgaga
     4441 tctacgccac cggcaagcga tggatgtgga agttccagca cctcgacggc cagaacgaga
     4501 tcaacgagct ccacgtgccg atgggccggc cgatcaaggt caccttcacc tccgaagacg
     4561 tgctgcattc gctgttcttc ccggcgttcc ggaccaaggc ggacgcgatt cccgggcgct
     4621 acagctccgt gtggttcacg ccgacgaagc ttggcgaata ccatctgttc tgcgccgagt
     4681 actgcggcac gaagcactcg ggcatgatcg gcagagtcgt cgtgatggaa ccggcggcgt
     4741 accaagcctg gctgagcggc tccgttccag gcatgtcgct cgccgcccgc ggtgagcggc
     4801 tgttcagcga gttggcgtgc aacacctgcc acctcgatga cgggcgggga cgaggcccgt
     4861 ctctggtgaa caagttcggg gcgtcggagc agcttgccag cggcggtgcg gtgccggtgg
     4921 acgaagccta cgtccgcgag tccattctca ggccgcagtc caagatcgtg gcgggatatc
     4981 agccgctgat gccgacgttc cagggtttgg tgagcgaaga gaacgtgatg gcgctcgtcg
     5041 aatacgtcaa gtcactgcgg gcaacggcgc aggcggccgc cccggccgcc gcgcacgccg
     5101 agagcacggc tcccggcgcg ccccaggaga gcaggtaagc catggatacg acggtcacgg
     5161 ccccgcgagt gacgtacctc aatcacgagc acacgctcgc gtcgtggctc ctgacgaagg
     5221 atcacaagcg gatcggcatc ctctacatga tcgtggtgac cttcgccttc ttcctgggcg
     5281 gcatcttcgc gtcgctgatt cggctcgagt tgacgacgcc ggcgggggac ctgctctcgg
     5341 cggacaccta caacaagatg ttcaccatgc acggcgtggc catggtgttc ttcgtgctca
     5401 tccccgccgt cccggccatc ctcggcaact tcgtcctgcc gctcatgctc ggcgcgaagg
     5461 acgtcgcgtt tccgaagctg aacctcataa gctggtacct cttcacgatc gggttcctgt
     5521 tcacggtgta cgcgctggtc gcgggcggtg tcgatacggg ctggaccttc tacaccccct
     5581 acagcagccg cgcctcgaac acgaacgtca tggccaccgg cctcggcgtc ttcatcaccg
     5641 ggttctcgtc gatcctcacc ggcctgaact tcatggtgac gatccaccgg atgcgggcgc
     5701 cggggctgac gtggttcagg ctgccgctgt tcgtgtgggc ggtctattcg acggcgctgg
     5761 tgatgatcct cgggaccccc gtgatcgcca tcacggtgct cgcgctcgcc atcgagcgct
     5821 tcctgcactt cggcatcttc gacgcggcgc tcggcggcga cccggtgctg ttccagcaca
     5881 tgttctggtt ctactcgcac cccgccgttt acatcatgat cctgccgccg atgggcgcca
     5941 tcagcgaggt gatcgcggcc ttctcgcgga agagcatctt cggctatgcc ttcgtggcgt
     6001 tttccagcct cgcgatcgca atcttcgggt tcctggtgtg ggcgcaccac atgttcgtgt
     6061 cgggaatctc ggtctactcg gcgctggtgt tctcgttcct cacctacgcg gtggccatcc
     6121 cgtcggctgt caagacgttc aactgggcgg ccacgatgta tcgcggcgcg atctggctga
     6181 aggcgccgat gatctgggcg atcgggttcc tgggcctctt cctcgtcggg ggcctcacgg
     6241 gcctcttcac cgccgcgctc ggcgtggacg ttcacctgca cgacacctat ttcgtcgtcg
     6301 cgcacttcca ctacatcatg gtcggcggcg ccatcatggg ctacttcgcc ggcctgcact
     6361 tctggtggcc gaagattacc ggacggatgt actccgacat ctgggcgaag gttgcggcca
     6421 tcatcgtgtt caccggcttc aacctgacct tcggtccgca gttcatcgtc ggctacctcg
     6481 ggatgccccg ccgctactac gcgtatcccg aggagttcca ggtgctgaac gtgctgtcgt
     6541 cggccggcgc gtcgatcctc ggcctgggct acacgctcat cgcgatctac ctcgtggcgt
     6601 cgctcttcaa gggagaacgc gtcggcgcga acccgtggca tgccacgggc ctcgagtgga
     6661 cgacgcagtc gccgccgctg ccgcacaact tcgagaagca gccggtggtg accgaggacg
     6721 cctacgagta cacgaagcgg gagatcaaag ttgtctgacg cactggcggc gcacggcgca
     6781 cacggacacg cgcatcatcc cgcgcagcag caccatttcg acacgctgga ccagcagcac
     6841 gaagcggcca cgctcgggat gtggctcttc ctcatcacgg aagtgctctt cttcggaggc
     6901 ctgttcctgg cctacatgct gtaccgcgtg tggtaccccg cagcctggtc cgaggcgagc
     6961 ctcgagctga acgtcgtgct cggcggcgtc aacacagcgg tgctgatcgc cagcagtctg
     7021 acgatggcga tggcggtccg gggcgcgcag accggtctgc gccgggcgac catgggctgg
     7081 ctggtcctga ccatggtgtt cggcctgacg ttcctcgtga tcaagtactt cgagtacgcg
     7141 gagaagatcg agcaccatca cgtgcctgga ccgacgttcg cgttcccggg accgaatgca
     7201 ggcccggcgg agatctattt ctcgctctac ttcctcatga ccggcctgca cgcgacgcac
     7261 atggtcatcg gcttcgggat tctctcggtc atcctctgga tggcgtataa gaaccgcttc
     7321 tcgcccgagt ggtacacgcc ggtcgagatc agcgggctgt actggcactt cgtcgacatc
     7381 gtctggatct tcctgttccc gttgctctac ctcgtggaca gggctcatca cttcgcgtga
     7441 ggcgctgatg gcaggacaca tctctcccaa aggcacctat tacgccatct tcggcatcct
     7501 gatggtgctg accctcgtca cggtctacgt cgcctaccag ccgctcggcg cggcgaactt
     7561 cccggtcgcg atcggcatcg ccatcacgaa ggcgacgctc gtcatcctgt tcttcatgca
     7621 cgcgaagtac agcagccggc tgacgaagct gttcgtcggt actgcgttct tcttcctggc
     7681 catcctcctc ggcctgtcgt tgaccgacta catgtcgcg
//
LOCUS       DBNGSH010000690         2140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2111444, whole genome shotgun sequence.
ACCESSION   DBNGSH010000690 DBNGSH010000000
VERSION     DBNGSH010000690.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2111444"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="AC16CY_15885"
     CDS             complement(<1..273)
                     /locus_tag="AC16CY_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355330.1"
                     /translation="MPSLLVKDFPHHMKNLRLPIFLFVVSATVAGCSRNATPTEQTAQ
                     QTAASGAAAEPDEEIPPPVYESALPEDMRSSVDQTFTGDWEEMIKRR"
     gene            complement(284..1075)
                     /locus_tag="AC16CY_15890"
     CDS             complement(284..1075)
                     /locus_tag="AC16CY_15890"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3355331.1"
                     /translation="MDTLPAVLLAMALVAPQSHEARDSSSDERYLAELRERVAGGDAE
                     AEVALGNLYESGQSVLPPDPARAAEWYRRAADKGHPGAQFNLANMYLDGLGVPKDAGE
                     AVAWYRKAADQGDTLAQFSLGSIYDTGAGRVPRDNASAALWYRRAADRGLATAQYRLG
                     MMHLHGRGVPRDTAQAVAWLRKAAHQDETDAQIALGTLLSPERRDVVEAHMWLNLAAS
                     RWKNEAQRLEAVTRRDGLAAVMTSGQLVEAYRRATEWQNAHALPQ"
     gene            1143..>2140
                     /locus_tag="AC16CY_15895"
     CDS             1143..>2140
                     /locus_tag="AC16CY_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3355332.1"
                     /translation="MSTAALETPKHSSAAGQGTALGILAAISVCHGLNDLMQSVVPAV
                     YPILKDVYRLDFGQIGLITFANQATASLLQPVIGAFTDARPKPYSLPIGMAFTLVGLL
                     LLSVAPSFGFILLAVSFVGIGSSVFHPESSRVARLASGGRHGFAQAFFQVGGNFGSST
                     GPLLAAFIVLPYGQRSIAWFAVTALCAMAILIRVGHWYRQHLAGAVKSAGARLRSAVP
                     AARVRVALVILVLLVFSKNVYLASLSSFYTFYLIQKFQLSVQSAQIHLFVLLGAVAAG
                     TFIGGPVGDRIGRKYVIWVSILGVLPFTLLLPYANLLGTEILTVVIGLILASAFSA"
BASE COUNT          295 a          742 c          697 g          406 t
ORIGIN      
        1 ccgtcgcttg atcatctcct cccagtcgcc ggtgaacgtc tggtccacgc tcgagcgcat
       61 gtcctcggga agggcggatt catagacggg cggcggaatc tcctcgtcgg gttcggcggc
      121 tgcgcctgag gctgccgtct gctgagctgt ctgctcggtt ggcgtcgcgt tccgcgagca
      181 gccggccaca gtggccgaga cgacgaacag gaagattggc agtctcaaat ttttcatatg
      241 gtgaggaaag tctttaacga gcagtgacgg caagtcaacc tcgtcactgg ggcagagcat
      301 gggcgttctg ccactcggtg gcccggcgat aggcctccac gagctgcccg gacgtcatca
      361 cggcagccag cccgtcgcgc cgcgtgacgg cctcgaggcg ctgtgcctcg ttcttccagc
      421 gcgaggccgc gaggttgagc cacatgtgcg cctcgacgac gtcgcggcgc tcggggctca
      481 gcagagtgcc cagcgcgatc tgcgcatcgg tctcgtcctg gtgggcggcc tttcgaagcc
      541 aggcgacggc ctgggccgtg tcccgcggga cgcctcgacc gtgcaggtgc atcatgccaa
      601 ggcggtattg tgccgtcgcc agcccccgat ccgctgccct gcgataccac agcgcggcgc
      661 tggcgttgtc gcgcggcacc cggccggcgc cggtgtcgta gatcgacccc agactgaact
      721 gcgcgagcgt gtcgccctgg tcggccgcct tccggtacca cgcgacggcc tcaccggcat
      781 ccttcggcac accgagtccg tcgagataca tgttggccag gttgaactgc gctccggggt
      841 gccccttgtc cgccgcgcgg cggtaccact cggccgcacg cgccggatcc ggcggcagca
      901 cgctctggcc ggactcgtag agattgccga gcgcaacttc cgcctcggca tccccccccg
      961 cgacccgctc gcgcagctcc gccaggtagc gctcgtccga cgacgagtcg cgagcctcat
     1021 ggctctgagg ggcaacgagc gccatcgcaa ggagaactgc tggaagcgtg tccacgctca
     1081 actcattcta ccggcccgtg ccggggccag gctctaacgc tacaattcag gtaacgattc
     1141 gattgagcac tgccgcactc gagacaccca agcattcgtc cgccgccggg caggggaccg
     1201 cgctcggcat cctcgccgcg atcagcgtgt gccacggcct gaacgacctg atgcagtcgg
     1261 tcgtgccggc ggtctatccg atcctcaagg acgtctatcg actcgacttc ggccagatcg
     1321 gcctcatcac gttcgcgaac caggccacgg cgtcgctgct gcagccggtg atcggcgcgt
     1381 tcacggacgc gagaccgaag ccctattcgc tgccgatcgg catggcgttc acgctggtcg
     1441 ggctgctgct gctctcggtg gcgccgagct tcggcttcat cctcctggcc gtgtccttcg
     1501 tgggcatcgg atcgtcggtc ttccatccgg agtcgtcgcg cgtggcgcgg ctcgcgtccg
     1561 gcggacgcca cggcttcgcc caggccttct tccaggtggg cggcaacttc ggttcgtcga
     1621 cggggccgct gctggccgcc ttcatcgtgc tgccgtacgg gcagcggagc atcgcgtggt
     1681 tcgccgtgac ggcgctctgc gcgatggcga tcctgatccg cgtcggccac tggtatcggc
     1741 agcatcttgc cggcgcggtg aaatcggccg gcgcgaggct ccgttccgcc gtgcccgcgg
     1801 cccgcgtgcg cgtcgcgctg gtgatcctgg tgctgctcgt cttttccaag aacgtgtacc
     1861 tggcgagcct gagcagcttc tacactttct atctgattca gaagttccag ctctcggtgc
     1921 agagcgcgca gatccacctg ttcgtgctgc tcggcgccgt cgccgcgggg accttcatcg
     1981 gaggcccggt cggggatcgc atcggccgca agtacgtcat ctgggtgtcc atcctcggtg
     2041 tcctgccgtt cacgctgctg cttccgtacg caaacctgct cggcaccgag atcctgacgg
     2101 tcgtcatcgg cctgattctc gcgtcggcct tttcggcgat
//
LOCUS       DBNGSH010000691         4723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4659242, whole genome shotgun sequence.
ACCESSION   DBNGSH010000691 DBNGSH010000000
VERSION     DBNGSH010000691.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4659242"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="AC16CY_15900"
     CDS             <1..575
                     /locus_tag="AC16CY_15900"
                     /inference="COORDINATES: protein motif:HMM:NF014804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355333.1"
                     /translation="VRAQIAIAAGAPLAWPQDGLTQRGHAIEARIYAEDPLRHDLPQA
                     GRLLLYREPSMPGVRVDAGVTEGSEVTVHYDPLLAKLIATGETREDARHRALAALRAY
                     PILGIRTNLELLIALLEHPRFIDGTADTGLLGAEGDAIRAGLSIDAGAEVAAIVAACR
                     SEPVASSAAGVSGSAARTVDPWTSLRGVRV"
     gene            568..975
                     /locus_tag="AC16CY_15905"
     CDS             568..975
                     /locus_tag="AC16CY_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR3355334.1"
                     /translation="MSEGGGEAAVSVVPLGNGRFQVDAGGTRRVAYAVGLRDTWVFLD
                     GHVFFVPLNPGPAAPPRLDERALTAPMPATVLAIHVAPGQAVARDEVLIVLEAMKMEL
                     PVRAPRDGRVATIDCKPGELVQAGAVLLELYGD"
     gene            1047..1457
                     /locus_tag="AC16CY_15910"
     CDS             1047..1457
                     /locus_tag="AC16CY_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355335.1"
                     /translation="MIVIGSCAHIACDGGAAPTLVGYAGEWIGTTPQGTPINFTVSAD
                     TVTSITLAYNFSADCSGTLTYTNLAIPIQSLDFPGPPTYEQPGFGYGANEGTRETYIA
                     GQFSPDRRSASGQFSLIRYGVCGTVMGTWSATRN"
     gene            complement(1492..2757)
                     /locus_tag="AC16CY_15915"
     CDS             complement(1492..2757)
                     /locus_tag="AC16CY_15915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355336.1"
                     /translation="MKAVSSIVGSSVVATNVVGEAFKSLKGRRLQASLSSFGIATGIA
                     AVVLLVSIVSGIHRYAVQQFGAVGGNVIMVTASPQRSLRDPRGFSVTLRVDDMKAVLA
                     GVDHYDLGMAENNTQRIVRTPRRASQGIQVRGITEHGFEMLDLRADRGRLFQRSEYEE
                     GRRVAVLGAALAADLFVQESPIGQTIVIGDWPFLVIGVLDWVGDPVAIAPIGPDNGLF
                     VPFEACAAAFLGNTNASSVRLRVTSAAAPADVIADTQATLDPLRKRRGETSGEFQVVS
                     SIERIQELTLVLNGLKAAVALVGSIGLFVGAVGVANVMLVSVRERRTEIGTRRAVGAT
                     RRAIFAAFLLEALAMTLTGGVVGIAGAWLLTKVAIFIPQVPVGARPHISLVTAAAALT
                     MLTLVGLVSGVWPARRAASVFPAEALRAE"
     gene            complement(2754..3905)
                     /locus_tag="AC16CY_15920"
     CDS             complement(2754..3905)
                     /locus_tag="AC16CY_15920"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3355337.1"
                     /translation="MRRLLKYVVALAFVAGLGVLAYVQLWPREAIAALGDQTFVAESA
                     QVEDVLLVTGLVRPAVTIELRAEASGIVEVVAAKEGDRVTAGQELVRLDSSLAQSGLQ
                     RSEANLRQAELQDAATKLDLDEDTLNLRRRNLDRAKALFAEGLVPREELEQRELELRV
                     AERTVERAKRNIEGSQARIAQARAEVDQARTQLQHTSIRAPFDAFVLRRQVEVGSGVA
                     GVSQSASGGSILMTLGDARESALYAKATAADARRLRPGQPARVRLDSDASVQIPAVLR
                     SVSTAGDVDQNSRLTTFPVVVALKTQPTGGWVNVPAQAEIVLSGTIDAVTIPERCVRS
                     DQSGRSFVIVQGQSGNRSRQVELGVIHQDRIQVRSGLEKGQTVVCRRTR"
     gene            3997..4248
                     /locus_tag="AC16CY_15925"
     CDS             3997..4248
                     /locus_tag="AC16CY_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355338.1"
                     /translation="MSEEIAAALVELIEKIGLLRRDMQQKKDLEKYEARFARIEQKLS
                     TLAERLSADDPDLAKAIRSVWQLPARSLAFRNVAHQKKP"
BASE COUNT          813 a         1612 c         1526 g          772 t
ORIGIN      
        1 tcgtgcgggc gcagatcgcc attgcggccg gggccccgct cgcctggccg caggacggcc
       61 tcacgcagcg cgggcatgcg atcgaagcgc gcatctacgc cgaagatccg ttgcggcacg
      121 acctgccgca agccggcagg ctcctgctct accgggagcc gtcgatgccc ggcgtgcgcg
      181 tggacgccgg cgtgacggaa gggagcgagg tgaccgtcca ctacgatccg ctgctcgcca
      241 agctgattgc gaccggcgaa acccgcgagg atgcccgcca tcgtgccctc gcggcgctgc
      301 gcgcctaccc gatcctcggg atccggacga atctggaact gctcattgcg ctgctggagc
      361 atccgcgatt catcgacggc accgctgaca cggggctgct gggcgccgaa ggagacgcaa
      421 tcagggccgg gctctcgatt gacgcggggg ccgaggttgc cgcgattgtg gctgcctgtc
      481 gatccgagcc ggtcgcttcc tccgccgcgg gcgtaagcgg ttcagccgcg cgaaccgtcg
      541 atccgtggac ctcgctgaga ggtgtgcgtg tctgaagggg gcggcgaggc cgcggtcagc
      601 gtcgttccgc tcgggaacgg gcgtttccag gttgacgccg gcgggacgcg ccgagtcgcc
      661 tacgcggtcg gcttgcgaga cacgtgggtg ttcctcgacg ggcacgtgtt cttcgtgccg
      721 ctgaatcccg gcccggcggc gccgccgcgg ctggacgagc gcgcgctcac cgcgcccatg
      781 ccggccaccg tgctcgccat tcacgtcgcg ccgggccagg ccgtggcgcg agacgaggtt
      841 ctgatcgtcc tcgaagccat gaagatggag cttccggttc gcgcgccgcg cgacggccgt
      901 gtcgcgacga tcgactgcaa gccgggcgaa ctggtgcagg cgggggcggt tctgctggaa
      961 ctgtatggcg actgacgatc tcttgaccac ccaggccgtg taggctaatc aacatgggtt
     1021 cgcgagacgc tcgtcgcctc gcaatcgtga tagtcatcgg cagctgtgcc cacatcgcgt
     1081 gcgacggcgg ggctgctccc acgctcgtcg gttacgcggg cgagtggatc ggaacgacac
     1141 cgcagggtac tccgatcaac ttcaccgtgt ccgcagacac ggtgacctcg atcacgctcg
     1201 cctacaactt ctccgccgac tgctccggca cgttgacata cacgaacctg gcgataccga
     1261 tccaatcact ggactttcca ggcccgccga cgtacgagca accgggattc ggctacggcg
     1321 ccaacgaggg gaccagagag acgtacatcg cgggacagtt ctcgccggat cgacggtcgg
     1381 cgtcggggca gttcagtctc attcgctacg gcgtctgcgg cactgtcatg ggcacgtgga
     1441 gtgccaccag gaactgatct cctgctcgat tcacgcagcc tccgggatca gctactccgc
     1501 gcggagcgcc tcggctggaa acaccgacgc cgcacgcctg gctggccaca cccccgagac
     1561 gaggccgacc agagtcagca tcgtcagcgc ggctgcggcg gtgacgagag atatgtgagg
     1621 acgggcgccg accggtacct gcgggatgaa gatcgcgacc ttcgtcagga gccacgcccc
     1681 ggcgatcccg acgacgccgc cggtcagcgt catggcaagc gcctccagga gaaaggcggc
     1741 aaagatggcc cggcgggtcg ccccgacggc ccttcgcgta ccgatctccg tgcgccgctc
     1801 gcgtaccgac acgagcatga cattcgccac ccccacggcg ccaacaaaca agccgatgct
     1861 gcccacgagc gcgactgcgg ccttgagccc attcagcacc agcgtcagct cctggatgcg
     1921 ttcgatgctg ctcaccacct gaaactcgcc gctggtttcc cctcgacgct tccggagcgg
     1981 atcgagcgtc gcctgagtgt cggcaatcac gtcggcaggc gccgccgcgg acgtcacccg
     2041 caaacgtacc gaggacgcgt tggtatttcc caggaacgcc gcagcgcagg cttcgaacgg
     2101 cacgaagagc ccgttgtccg gaccgatcgg cgcgatcgcg acgggatccc caacccagtc
     2161 cagcacgccg atcaccagaa acggccagtc gccaatcacg atcgtctggc cgatcggcga
     2221 ttcctgcacg aagagatcgg ccgcgagcgc cgcacccaat acagcgaccc gccgtccctc
     2281 ctcgtactcg ctccgctgaa acaggcggcc gcgatcggct cggagatcga gcatctcgaa
     2341 cccgtgctcg gtgatcccgc gcacctgtat gccctggctc gcccggcgcg gtgttctgac
     2401 gatcctctga gtgttgtttt ccgccatacc gaggtcgtag tggtccacgc ctgcgagcac
     2461 ggccttcata tcgtccacgc gcagcgtcac tgaaaacccg cgcggatcgc gcagcgaccg
     2521 ctgtggcgag gcggtgacca tgatcacgtt tccaccgacc gcgccgaact gctggaccgc
     2581 gtagcggtgg atgcccgaca cgatggacac gagcaggacg accgcggcga tcccggtggc
     2641 gatgccgaac gaggacaggg acgcctgcag acgacggccc ttcaggctct tgaaggcttc
     2701 gccgacgaca ttcgtcgcga ccaccgacga gccgacgatg ctcgacactg ccttcatcgc
     2761 gttcgccggc acacgacggt ctggcccttc tcgagacctg accggacctg gatgcggtcc
     2821 tgatgaatca cgcccagctc gacctgccgg ctccgattgc cgctctggcc ttgaacaatc
     2881 acgaacgacc ggccggattg atcgctgcgc acgcagcgct cgggaatcgt gaccgcgtcg
     2941 atcgttcctg acaggacgat ctcggcctgc gcggggacgt tcacccatcc gcccgttggc
     3001 tgagtcttga gcgcgaccac gaccggaaac gtggtcaatc gcgaattctg atcgacatcg
     3061 cccgccgtgg acaccgaacg cagtaccgcg ggaatctgca cggaggcgtc ggagtcgagc
     3121 cggactcggg ccggctggcc ggggcgcagg cgtctggcat cggccgccgt ggctttggca
     3181 tagagcgcag attcacgcgc gtcgccgagc gtcatcagaa tgctgccgcc ggacgccgac
     3241 tggctgacgc cggccacgcc gctgccgacc tcgacctgac gccgcagcac gaacgcgtcg
     3301 aacggggcgc ggatggacgt gtgctggagc tgcgtgcgcg cctggtccac ctcggcacgc
     3361 gcctgcgcga tgcgcgcctg actgccctcg atgttgcgct tcgctcgctc gacggtgcgc
     3421 tcggccaccc tcagctcgag ctcgcgctgc tcgagctcct cccgcggcac gagcccttcg
     3481 gcaaacagcg ctttcgcgcg atcgaggttg cggcgccgaa gattgagcgt gtcctcgtcc
     3541 aggtccagct tcgtcgcggc gtcctgcagc tccgcctgcc ggagattggc ttccgaccgc
     3601 tggagccccg actgcgcgag gctggaatcg aggcgcacga gctcctgccc cgccgtgacg
     3661 cgatcgccct ctttggccgc cacgacctcg acgatgccgg aggcctccgc cctgagctcg
     3721 atcgtgacgg caggcctgac gaggcccgtg acgagcagca cgtcctccac ctgcgcgctc
     3781 tcggccacga acgtctggtc gccgagcgct gcgatcgcct cgcgcggcca gagctgcacg
     3841 taggccagga cgcccagtcc cgccacgaag gccagcgcca cgacgtactt cagcaggcgt
     3901 cgcatgaaat ggtgtccaga gtacgatgcc cgtccggcga ttgcaaagac gaagcggggt
     3961 cacccgtggc aggatcgagg ctgagggaac gtccggatgt cagaggagat tgcagcggcg
     4021 ctcgtcgaac tgatcgagaa aatcggccta ctgcgccgcg acatgcagca gaagaaggat
     4081 ctcgagaagt acgaggcccg cttcgcgcgc atcgagcaga agctgtcgac gctggccgag
     4141 cgcctgtcag ccgatgaccc tgacctcgcg aaggcgatcc gctcggtgtg gcagcttccc
     4201 gcgcggagcc tcgcgttccg caacgtggcg catcagaaga agccctagcc cattcgtgat
     4261 tggtgattgt cgatccttga ttcgttgatg atttgtgatt ctggattggt gattaaccct
     4321 gatgtgacaa tagcgggtgg cgcgtacgta gtgtccggct tcagccggac ctcgcaggcg
     4381 cgatagacat cgcaggtgtc ctcacgtgtc gggcccgtgt ggagatttca ctcccggtga
     4441 gttctcgatc agggcgatcc cgcccggccg atggtccggc tgaagccgga cactacgaca
     4501 gacaacgcct gcgggctcct tgacgtcggg cgtggctatt gtcacatcga ggtcaagtcg
     4561 agggttcggt acccaatcgt caatcgacaa tctgcaatca acaacgattc aacaatcacc
     4621 aatcaagaat cactaatctt tcgagcccgc ggtctgcagc gacaggtacc gctcgtagcc
     4681 catctccttg atcatgtgga gctgcgactc gagccagtca acg
//
LOCUS       DBNGSH010000692         1672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_74109, whole genome shotgun sequence.
ACCESSION   DBNGSH010000692 DBNGSH010000000
VERSION     DBNGSH010000692.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_74109"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="AC16CY_15930"
     CDS             complement(<1..1655)
                     /locus_tag="AC16CY_15930"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355339.1"
                     /translation="MDDLVHDIRYGARALWRNPGFAASATLVLAVGIGLNLAFFHVVK
                     ATLSASLPLRDGSTLVRIVRQTPEGERWAMTMTALAFYREHTNVFSYIVGERQATEAV
                     QVQQDDEDARARFVSGNYFLDLGVTPALGRVFGPSDDQPGGPLVAVLSDGYFKRRFGG
                     DPGVVSRTVTINGASVIVLGVLPAEFVSVTMSRADLWLPDAGRAGLVATTRRPAGVES
                     PDTALVAQPRPGVSRDVISAQLAAVDADLRSQLPGVFEKGDLVRARPVVEDERRGNGL
                     AIGTTLAFLVLLTSCANLGNMMLARGVTRRREIDTRIAVGASRSRLVRQLMTEALLLS
                     GMGAAAGLALASVGGRLLVVNFAGQLNVQVTTDLTVVASAGLLGLVCAIVFGLAPARH
                     VSRQRPPATKARQTLVAVQVATSCLLLVLAGLLVRGSQRQLELAGLADHTSVIVVEPR
                     LEERAFPGNSARAAIDDMAARVRAVGDVVEVAVAADPIYGTAVLSAERAPVVLQSRVD
                     PAYFDVMDIAVLRGRMFTPGERGVAVISRSAERAEFEGGDGLGR"
BASE COUNT          294 a          602 c          555 g          221 t
ORIGIN      
        1 cggcccagcc cgtccccacc ctcgaactcg gcccgctcgg cggagcggct gatcaccgcg
       61 acgcctcttt cgccgggcgt gaacatccgg ccgcgcagca ccgcaatgtc catgacatcg
      121 aagtacgcgg gatccacgcg cgactgcaga acgacgggtg cgcgctcggc gctgagcacg
      181 gctgtcccat agatcggatc ggcggcgacg gccacctcga caacgtcacc gacggcgcga
      241 acgcgtgcgg ccatgtcatc gatggctgcc cgtgccgagt tgccgggaaa cgcacgctcc
      301 tcgagccgcg gttcgaccac gatcaccgac gtgtgatcgg cgaggcccgc cagctcgagc
      361 tgacgctgcg agccgcgcac caggagaccg gcgagcacca gcagaaggca gctcgtggcg
      421 acctgcacag ccaccagcgt ctgcctcgcc ttcgtggccg gcggacgctg tcggctgacg
      481 tggcgggccg gcgcgaggcc aaacacgatc gcacagacaa gaccgagcag gccggccgac
      541 gcgactaccg tcaggtcggt cgtcacctgc acgttgagtt gtccggcaaa gttgacgacg
      601 agcagccggc cgccaacact cgcaagcgcg agtcccgcgg ccgcccccat ccccgacagc
      661 agcagcgctt cggtcatcag ctgtcgcacc agccggctgc ggctcgcgcc cacggcgatg
      721 cgcgtgtcga tctcgcggcg ccgggtgacg ccgcgggcca gcatcatgtt cccgaggttg
      781 gcgcacgagg tcagcagcac gagaaacgcc agcgtggtgc cgatcgcgag cccgttacct
      841 cgccgctcgt cctcgacgac cggccgtgca cgcacgagat cgcctttctc gaatactccg
      901 ggcaactgcg accgcaggtc ggcatcgacc gccgcgagtt gggcggagat gacatcgcgg
      961 ctcacgccgg ggcgcggctg cgcgacgagc gctgtatccg gcgattccac accggccggc
     1021 cggcgggtcg tcgcaacgag gccggcgcga ccagcatccg gcaaccagag atcggcgcgg
     1081 ctcatcgtca cggagacaaa ctcggccggg agcacgccca ggacgatcac gctggcgccg
     1141 ttgatcgtga ccgtccgcga gacgacgccg ggatcgccgc cgaagcgccg cttgaagtag
     1201 ccatcgctga gcactgcgac cagcggccct ccgggctggt cgtcagaagg tccgaacacc
     1261 cggcccagcg ccggagtgac gccgagatcg aggaagtagt tgccggagac gaacctggcg
     1321 cgcgcatcct cgtcgtcctg ttgtacctgc accgcctcgg tcgcctggcg ctcgcccacg
     1381 atgtaggaaa aaacgttcgt gtgctcccga tagaaggcca gcgcggtcat cgtcatcgcc
     1441 cagcgctccc cttcgggcgt ctggcgaacg atccgcacca gcgtgctgcc gtcccgcaac
     1501 ggcaagctcg cagagagcgt cgctttcacg acgtggaaga aggcgagatt cagcccgatg
     1561 ccgacggcca gtacgagcgt cgccgacgca gcgaagccgg gattccgcca gagcgcgcga
     1621 gcgccgtagc gaatgtcgtg caccaggtcg tccatccatc gtctgaagcg gg
//
LOCUS       DBNGSH010000693         2030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_923624, whole genome shotgun sequence.
ACCESSION   DBNGSH010000693 DBNGSH010000000
VERSION     DBNGSH010000693.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_923624"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="AC16CY_15935"
     CDS             complement(<1..1070)
                     /locus_tag="AC16CY_15935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3355340.1"
                     /translation="MIHRLAAALTLAAGATALAAQQATFRASIEAIEIDVYVTDSAGK
                     PVTGLTADDFTVLENGEPRPIRAFSAVDFPIHLPGSIKPPLARPDVLSNHGPPGRVYL
                     FAFGPLGGSSTLRARHFARQFIEQHFGPNDVGAVAVLGPGRPDGVQDFTSSPRLLLDA
                     IDRFTGSHASGAPLPSLSLASVSTENSLAGTTLVSLSRTQVAPSPFASGPSIRRGGRS
                     DYLERLRMLTEMTARMPGHHKALLLFTAAPPVDMYDLIDYQGGVLSVAGEDAHAAMSA
                     ATRSNLAIYPIDPNGLTTDLLPLGEVGLYRALADATGGAALINSNNWPDLFDTIVRDN
                     SSYYMLGFQPGYDRDDGRYVRL"
     gene            1133..1903
                     /locus_tag="AC16CY_15940"
                     /pseudo
     CDS             1133..1903
                     /locus_tag="AC16CY_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575680.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
BASE COUNT          329 a          675 c          712 g          314 t
ORIGIN      
        1 tcgagccgca cgtagcggcc atcgtcgcgg tcgtagccgg gttggaaccc gagcatgtag
       61 taggagctgt tatcgcgcac gatggtgtcg aagaggtcgg gccagttgtt ggagttgatc
      121 agcgcggcgc cgcccgtggc atccgccagc gcccgataca accccacttc cccaagtggc
      181 aggaggtcgg tcgtgagccc gtttggatcg atcgggtaga tcgccagatt gctccgcgtc
      241 gcggccgaca tcgccgcgtg cgcgtcctcg ccggcgacgc tcaggacgcc gccctgatag
      301 tcgatgaggt cgtacatgtc gaccggcgga gccgcagtaa acagcaggag cgccttgtgg
      361 tgccccggca tccgtgccgt catctcggtc aacattcgca ggcgctcgag gtagtcggac
      421 cttccccccc tccgtatcga agggccgctt gcgaagggcg atggagcgac ctgtgtgcgt
      481 gacagcgaaa ccagcgtcgt gcctgccagc gagttttccg tcgagacgct ggccagcgag
      541 aggctgggca acggcgcccc actcgcatgt gagcccgtga atcggtcgat cgcatcgagc
      601 agcagccgcg ggctgctcgt gaagtcctgc acgccgtcgg gccggccagg gccaagcacc
      661 gcgacggcac cgacatcatt gggtccgaag tgctgctcga tgaactgcct cgcgaagtga
      721 cgggcgcgca gggtgctcga gccgccgagc gggccgaacg cgaacaggta gacgcgccca
      781 gggggaccgt ggttcgacag gacgtcgggc ctggcgagtg gcggcttgat cgacccgggc
      841 agatggatcg ggaaatcgac ggccgagaac gccctgatcg gcctcggctc gccgttctcg
      901 agcacggtga agtcgtctgc cgtgaggccg gtgactggct tgccggcgct gtcagtcacg
      961 tagacgtcga tctcgatggc ttcgatggag gcgcgaaagg tcgcctgctg ggcggcgagc
     1021 gccgtggcgc cggcggcgag cgtgagcgcg gcagcgagac gatggatcat ggtccctcca
     1081 ggctcagcga aagatgctga catcacgggc agaccgagtc aaactccccc cgatgctgac
     1141 cgcctccggc cgccatgccg ctgccgcagc agtccttgtc gttctgctgt gccccgccgc
     1201 ctgggcgcag cagatcaccg tcatgacctc gggcggcttc accgcgcctt atctcgagct
     1261 cgtcccggag ttcgagcgca cgacgggaag cacggtcacg agcgtgttcg gcgcgtcgat
     1321 gggcggtgcg cccgactcga tcccggtccg cctcgcacgc ggcgaagcag ccgacgtcgt
     1381 gatcctgacc tccgatgcgc tcgaggcgct cgcagcgcag ggccgcatcg ccgccgggac
     1441 gcgcgtcgat ctcgtgcgat cgcgaatcgg catggccgtg cgcgcgggag cgccgaggcc
     1501 cgacatcagc acggtcgagg cgctcacgag gacattgctg tcggcgaaat cgatcgcgta
     1561 ctcggcaagc gccagcggga cgtatctctc aaccgagctc tttccgcgcc tgggtatcgc
     1621 cgatcggatc aggacgaaga gccgtcgcat cgaaagcgaa cgcgtcggcg cggtcgtcgc
     1681 ccgcggcgac gccgagatcg gttttcagca ggtcagcgag ctgctgccga tcgcgggcat
     1741 cgactacgtc gggccgctgc cggacgaggt gcaacgcgtg tccacgttct ccgcggcggt
     1801 cgtcacggga gcaccagagc ccgagcgagc ccgcgcgctc attgaatttc tggcatcgcc
     1861 cgccgcgcta ccgaccatcc gcaagtacgg cctcgagcct gcgaaagggt cagaccccga
     1921 ctcagaattg caggcggtcg ctgcacgccc gccgctccgg cagcttcgca tcgtggcgcc
     1981 ggccgcaccg ggcgggggat gggaccagac cgcgcgggtg atgcagcagg
//
LOCUS       DBNGSH010000694         5595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_413961, whole genome shotgun sequence.
ACCESSION   DBNGSH010000694 DBNGSH010000000
VERSION     DBNGSH010000694.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_413961"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="AC16CY_15945"
     CDS             complement(<1..521)
                     /locus_tag="AC16CY_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355341.1"
                     /translation="MDSRSVPACVLAAVVAIMPPVPAAGQKASQAGAGLSLAVSAGPL
                     NPGSVARLTVTAPDMLTALEGQAFGRAVHFWPSTTASTWHGLVGVGLATSSGAYNVTI
                     QATRADGGKATAKVALRVQPKQFETRRLKVDPSLVNPPADQAVRIERESKAVAEAFAA
                     PSSERLWRGPFRAP"
     gene            complement(591..947)
                     /locus_tag="AC16CY_15950"
     CDS             complement(591..947)
                     /locus_tag="AC16CY_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355342.1"
                     /translation="MATLLDVVLAFSTWIKIGWVVWFAWGAWQLVWYRQERHQAATFR
                     LPRRFNILDPRTPESVGLRSVAPEPSADQLITPEPEPIVHRFVTPDPFDEVPPHVADA
                     AANSSVPAGMGAPHSS"
     gene            complement(954..1736)
                     /locus_tag="AC16CY_15955"
     CDS             complement(954..1736)
                     /locus_tag="AC16CY_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355343.1"
                     /translation="MHLLRGPGAVATILHIRSREKSGVTTSATGPRWTAFLKRHSWGL
                     STCAFVIAGAGLAYATFDEVDRLTRLRAPTPPEVTTDVIVRNAKDEEGNRASFRILLF
                     TDEFRWKMSSYEELESFDHPAFTPEMKSVLTRAKEVIVVGASSEEVAPGLAQQAARKK
                     EETRAARRASRIALWVREALTKPIPIRKLNVGHHTSTGGGDDTSDQRRVVIILVLDRQ
                     DGTNVDEALRAAMTHESVNSPIFKVLLSRYSLATGKSFTWVE"
     gene            complement(1886..2413)
                     /locus_tag="AC16CY_15960"
     CDS             complement(1886..2413)
                     /locus_tag="AC16CY_15960"
                     /inference="COORDINATES: protein motif:HMM:NF025692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HIR3355344.1"
                     /translation="MAAVSCLVAAQPAYPQGNQTLRVTPLVRMEEERVFVSFELAGGL
                     TDEVRAAIQSGLQTTFTYAIELRLDVPAWFDRTISTATIANRVEYDNLTRVYNIVRLL
                     DGRTEKADVTQSDDVVRTWMTSLERFPLFSTTRLEPNREYYIRVSASARPSNGMFFWP
                     FGSGPSAQSRFTFIR"
     gene            2595..3650
                     /locus_tag="AC16CY_15965"
     CDS             2595..3650
                     /locus_tag="AC16CY_15965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3355345.1"
                     /translation="MRKFSIATVVLLGVAVLEHRVEAQALTFSLFEKYLDSFRAAAGI
                     PGMSGAVVQNGVVVWQRGFGRRDVEAGLPATPETPYLIGGLSQTVGATLLLRHCVDSG
                     LASLTDRVQQFAPLYPEPETTLNQLLSHTAAGGGSYRYSLERFAGLTGVIERCSGVPY
                     RALVASEIFDRFAMTDSAPDQALSTTTPQEADWLDQAHLTQYAQVVGRMATPYRVMAG
                     RAQRAPATTPRRADASDGIISSARDLAKFDVMLTSPGVLLDPLTRDYAWTQSFEGDLP
                     LPTGLGWFVQNYNGEPVVWQFGRGSHSALIVKAPNRGLTFILLANSDGLGAPFAVEPR
                     DVTTSLFAALFLRFFVP"
     gene            3779..4270
                     /locus_tag="AC16CY_15970"
     CDS             3779..4270
                     /locus_tag="AC16CY_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355346.1"
                     /translation="MLPDLEQSAGGAHLIFGGEAGWWSSGLFGIETDVAFAPAFFERT
                     EGVLVTDSNVLTIGMDVVVATPLSITRESLRPYLIGGLGWMHSSIEERQGLFPELFGR
                     AQNSVAVNVGGGAMGFFTPRTGVRFEVRHFRSLKRDTDLLTGETGSLLSYWRATVGIV
                     IKR"
     gene            complement(4292..4939)
                     /locus_tag="AC16CY_15975"
     CDS             complement(4292..4939)
                     /locus_tag="AC16CY_15975"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR3355347.1"
                     /translation="MKSKPSSSSAGKGGDREVARCWWCLGDPEYVRYHDEEWGRPVTD
                     DRRLFEKICLEGFQAGLSWLTILKKRDRFRRAFAGFDIDRVSRFTARDVTRLLGDEGI
                     VRHRGKIESTINNARRAIELRGEQGSLSAYFWGWKPAESARPRRITRSVLARMATTPE
                     STALSRDLRKRGWTFVGPTTVYAFMQAMGLVNDHVESCEFRHRSTPNARLPTPKI"
     gene            complement(4914..>5595)
                     /locus_tag="AC16CY_15980"
     CDS             complement(4914..>5595)
                     /locus_tag="AC16CY_15980"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3355348.1"
                     /translation="SLALGSGLVTPLDLTAAYGVFPGRGVRVRPRGVAQIEGADGSIV
                     ERVSVEREQVLSPQVAFQMVTMLQDVTQRGTAASVQRHNLPMAVAGKTGTTNESHDAW
                     FVGFSSSIVVGVWIGFDDPQTIRGDASGSRVALPIWADFMRRTARRFPGHSFERPPRL
                     HTEVMCQVSYHRALDGCPSYVEYFKPGDDVPSQLCELHSGSLGQRAERALRELLRGIG
                     SEIEAIFK"
BASE COUNT          959 a         1843 c         1809 g          984 t
ORIGIN      
        1 ggagcgcgga acggccctcg ccacagccgc tcggacgacg gcgcggcaaa cgcctctgca
       61 acagcctttg attcccgctc gatccgcacg gcctgatcgg cgggagggtt caccagcgac
      121 ggatcgacct tcagccggcg ggtttcgaat tgctttggct gaacccgcaa ggcgaccttt
      181 gccgttgcct tcccgccgtc ggccctggtg gcctgaatcg tgacgttgta tgcgccagac
      241 gatgttgcga gcccgacgcc aacgagaccg tgccacgtcg aggccgtcgt ggacggccag
      301 aagtgcacgg cccggccaaa ggcctgccct tcaagcgccg tcagcatgtc tggcgccgta
      361 accgtcagcc tggcaacact gcccggattc aaaggacccg ctgagacggc cagcgagagc
      421 cctgcaccgg cctgtgacgc cttctgaccg gctgcgggaa caggcggcat gatggcgacc
      481 accgcagcga ggacgcatgc gggcacagag cgagagtcca tacagccctt gtcagcgtac
      541 gctaatcgag gtgtcggcga aagggcgccc gaccttcaac gagcgaggtc ttaggacgag
      601 tgcggggcgc ccatgcccgc gggcacggag ctgttggcgg ccgcatcggc aacgtgcggc
      661 ggcacctcgt cgaagggatc cggcgtgacg aatcgatgga cgatcggctc gggctcgggc
      721 gtgatcaact gatcggcgct gggctcgggc gcgacggagc ggagaccaac cgactcgggc
      781 gttcgggggt cgaggatgtt gaacctgcga ggaagcctga acgtcgccgc ctggtggcgc
      841 tcctgcctgt accagaccaa ctgccaggcg ccccaggcaa accagacgac ccacccaatc
      901 ttgatccagg tcgagaacgc gagaacgaca tcgaggagtg tggccatgta ttgctactcc
      961 acccaggtga acgacttgcc ggtcgcgagc gagtaacgcg acagcaggac cttgaagatc
     1021 ggcgagttga cgctttcatg cgtcatcgcc gcccgcagtg cttcgtccac gttcgtgccg
     1081 tcctggcggt cgagaacgag aatgatcacg acgcgacgct ggtcggaggt gtcgtccccg
     1141 cccccggtag acgtgtgatg cccgacattc agcttgcgaa tgggtatcgg cttggtcagc
     1201 gcctctctca cccacaacgc gatgcggctc gcccgccggg ccgccctcgt ttcttccttc
     1261 ttccgcgccg cctgctgagc caggcctgga gcgacctcct ccgaactggc gcccacgacg
     1321 atcacttcct tggcccgtgt caggaccgac ttcatctccg gagtgaaggc gggatggtcg
     1381 aagctctcga gctcttcgta ggaagacatc ttccagcgga attcgtcagt gaacagcagg
     1441 attcggaacg aggcgcggtt gccctcctcg tccttggcgt tccgcacgat cacgtcggtc
     1501 gtgacctcgg gaggcgtcgg cgcgcgaaga cgcgtcaggc gatcgacctc gtcaaaggtg
     1561 gcgtaggcca ggccggcgcc cgcaatcacg aaggcacacg tcgagagccc ccagctatgt
     1621 ctcttgagga aggcggtcca ccgaggcccg gttgcagacg tggtcacgcc gcttttctcc
     1681 ctcgatcgga tgtgcaggat tgtcgccacc gctccgggac ctcggagcag gtgcatagtc
     1741 gtgcagaact ggctcggcag accggcagcc gggccgggcg cactgacgta aatcgatgtc
     1801 gccgcacaag tctggcgaac gggcccgaac aggccgctgc catggctggc agcggggcca
     1861 gaaccgtgct cgactgacgg aaggctcagc ggatgaaggt gaagcgcgac tgggccgatg
     1921 gaccgctgcc gaacggccag aagaacatgc cgttgctcgg ccgggcgctg gcgctgacgc
     1981 ggatgtagta ctcgcgattc ggctcgaggc gggtcgtgct gaagagcggg aatctctcca
     2041 ggctcgtcat ccacgtccgg accacgtcgt cactctgcgt cacgtcggcc ttctcggtgc
     2101 ggccatcgag cagtcgcacg atgttgtaga cgcgggtcag gttgtcgtac tcgacgcgat
     2161 tcgcaatcgt ggcagtcgag atggtccggt cgaaccatgc cgggacgtcg agccgaagtt
     2221 ctatcgcgta ggtgaacgtc gtctgcagac cgctttggat cgcggcgcgc acttcgtctg
     2281 tcagaccgcc cgccagctcg aaggagacga acacacgctc ctcctccatg cgcacgagcg
     2341 gtgtgacgcg cagcgtctgg tttccctggg ggtacgcggg ttgagcagcg acgaggcagc
     2401 tgacggcagc caacgcgcag aaacgccagc gagcgcgtcg ttgcgcagcg cgaatctcac
     2461 tcagttgtgc agaaacagaa cgcatccggc agatcgccgc ctcaatgtag cacacggcca
     2521 cgatcctgtg aacccggaat tgtcacccgg tgcgcccgtg gatgctatga tcccgatcgg
     2581 attcacccgc cactgtgcgc aagttcagca ttgctacggt cgtgctgctc ggcgttgctg
     2641 tcctcgaaca tcgcgtcgag gcccaggcgc tcaccttctc cctgttcgag aagtatctcg
     2701 actcgttcag ggcggcggcg ggtatacctg gcatgtcggg cgcggtcgtc cagaacggtg
     2761 tggtggtgtg gcaacggggc tttggccgcc gcgatgtcga ggccggcctg ccggccacgc
     2821 ccgaaacgcc ttacctgatc ggcggactgt cgcagaccgt cggcgccacc ctgctgctca
     2881 gacattgcgt ggactcggga ctggcaagtc tgaccgaccg cgtacagcaa tttgcgcccc
     2941 tgtatcccga gccggagacg acgctgaatc aactgctgag ccacacggcc gcgggcgggg
     3001 gaagttacag gtattcgctg gagcgatttg cgggtcttac cggcgtcatc gagcgctgca
     3061 gcggagtccc gtaccgggcg cttgtggcct cggagatctt cgaccgcttt gcgatgacgg
     3121 attctgcgcc ggaccaggca ttgtcaacga cgacaccgca ggaagccgac tggctcgatc
     3181 aggcgcatct gacccagtac gcgcaggtgg tcggccggat ggccacgcca taccgcgtca
     3241 tggctggccg agctcaacgc gcccccgcga cgacaccgcg ccgggccgat gcttccgacg
     3301 gcatcatcag ctcggcgcga gaccttgcga agttcgacgt gatgctcacg tccccggggg
     3361 tccttctcga tccactcacc agggattacg cctggacgca gtcattcgaa ggcgatctgc
     3421 cccttcccac tggactgggt tggttcgtcc agaactacaa cggggagccg gtcgtctggc
     3481 agttcggccg gggcagccat tcggcgctca tcgtcaaggc gcccaaccgc gggttgacgt
     3541 tcatcctgtt ggccaacagc gacggcttgg gcgcgccatt cgcggtcgag cctcgcgacg
     3601 tgacgacctc gctatttgcc gcactattcc tgagattctt cgtgccttga gggcgtacgt
     3661 cactggccgt ctggcgcggg tcgttcttgt ttcgctcgtc ctctgcggct cgccggccga
     3721 cgcacgcgct gactggctgt tcacgccgtt tcttggcagt acgttcgccg gatcgaccgt
     3781 gctgcccgat ctcgagcaga gcgcaggagg agcccacctc attttcgggg gcgaggccgg
     3841 ctggtggagt tccggcctgt tcggcatcga aaccgacgtc gcattcgccc cggcgttttt
     3901 cgagcgtact gagggcgtac tcgtcaccga cagcaacgtg ctgaccatcg ggatggacgt
     3961 ggtggtcgcc actccgctca gcatcacacg cgaatcgctg cgcccctatc tgataggcgg
     4021 tctcggctgg atgcactcct cgatcgagga gaggcagggg ctgtttcccg aactcttcgg
     4081 gcgggcgcag aactcggtcg ccgtcaacgt cggcggcggc gccatgggct tcttcacccc
     4141 ccgaaccggc gtgcgcttcg aggtccggca tttccgcagc ctgaagcggg acaccgacct
     4201 gctcactggc gagaccggct ccctcctcag ctactggcgt gcgactgtcg gcatcgtcat
     4261 caaacgatga gccgcgcggc ggaatgggga ttcagatctt gggagttgga agtcgggcat
     4321 tgggcgttga ccgatggcgg aactcacagc tttcaacgtg atcgttgacc agccccatcg
     4381 cctgcatgaa ggcgtacact gtcgtcggtc ccacgaacgt ccagccgcgt ttccgaagat
     4441 ccctgctgag cgccgttgat tcgggcgtcg tcgccatccg cgcgagaacg gatcgggtga
     4501 tgcggcgcgg ccgtgccgac tccgccggtt tccagcccca gaagtaggcc gagagcgacc
     4561 cctgttcacc gcgcagctcg atcgctcgcc gggcgttgtt gattgtcgat tcgatcttgc
     4621 cgcgatgccg gacgatgccc tcgtcgccca gcaggcgcgt gacgtcgcgc gccgtgaagc
     4681 gcgacactcg atcgatgtcg aaaccggcga aggcccgccg gaagcggtcc cgtttcttca
     4741 gaatggtcag ccagctcagg ccggcctgaa agccctcgag acagattttc tcgaagagac
     4801 gccggtcgtc ggtcaccggt ctgccccact cttcgtcgtg gtagcggacg tactccggat
     4861 cgccaaggca ccaccagcag cgggcgacct cgcgatcccc gcccttgcct gcgctacttg
     4921 aagatggctt cgatttcact gccaatcccg cgcaacagtt cgcgaagcgc gcgctcggca
     4981 cgctggccga ggctgccgga gtgcagctcg cagagctgac tcggcacgtc gtcacccggc
     5041 ttgaaatact ccacgtagct cgggcagccg tcgagagcgc gatgatagga cacctggcac
     5101 atgacttccg tgtgcaggcg cggcgggcgc tcgaaggagt gtcctggaaa acgcctcgcc
     5161 gtccgccgca tgaagtcggc ccagatcggc agcgccacgc gcgaacctga cgcgtcgccg
     5221 cgaatggtct gtggatcgtc aaatccgatc cagacgccga ccacgatgct cgaggagaaa
     5281 ccgacgaacc atgcatcgtg cgactcgttg gtcgtccccg tcttgccggc cacggccatc
     5341 ggcagattgt gccgctggac ggacgcggcc gtcccgcgct gcgtgacatc ttggagcatc
     5401 gtcaccatct ggaacgcgac ctgcggcgac agcacctgct cccgctcgac ggacactcgc
     5461 tcgacgatgc ttccgtcagc gccctcgatc tgagcgacgc ctcgcggccg cacgcggact
     5521 ccccttcccg gaaaaacgcc atacgccgcc gtgaggtcca gcggcgtcac gagcccgctg
     5581 ccgagggcaa gcgac
//
LOCUS       DBNGSH010000695         7091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_754148, whole genome shotgun sequence.
ACCESSION   DBNGSH010000695 DBNGSH010000000
VERSION     DBNGSH010000695.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_754148"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="AC16CY_15985"
     CDS             <1..1599
                     /locus_tag="AC16CY_15985"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR3355349.1"
                     /translation="VIVKDRSGRQTMEGRSGSATMERLQDVVLLDSAVHLLREGEVID
                     ADHARITLAEGEQVITSLQLRGRSSVRGDGGPLESMRADEIDLDYRDDGKTVEATALR
                     GGASVTMAADEQTSGRQMSGNTLDLQLAADGALTSLEGRGMVSFEIPAVGRSPARRIY
                     AGALSATGEPGKGLVAARFRQNVEFREQGSGAPSREARSQALFVQLDGDAVSSAQFSE
                     GVTFDEQALHATAAEATYQPGKSLLSLVGSKGAGEPTVTDERIRVDARSIEVMLDGHG
                     MIARDNVRTVLRGRTAGRSGRAAENGRLPGLFKEGDPVSASAARLDYEGQAGRAVYTG
                     NATLLQGDTAIRGGVVSIDQEKGDLAVTGSARSTLALDGGKADGQADEIRYDEAARQV
                     TYSVTPQAPTTVGSGVSAAKAAAVRPRVARLNGPQGDLRAQRIVIALARDGNEIERLE
                     GYDDVTMVQGERTAVGARLTYDAREESYVMSGRGGTLVTIRAIVRGSCRETAGETLTF
                     FKSNDKIVVGGRTEDKPCVAAPAR"
     gene            1612..2340
                     /gene="lptB"
                     /locus_tag="AC16CY_15990"
     CDS             1612..2340
                     /gene="lptB"
                     /locus_tag="AC16CY_15990"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR3355350.1"
                     /translation="MPSTLRTHELTKSYSGRTVVRGVSIEVASGEIVGLLGPNGAGKT
                     TTFYMTVGLTSPDSGRVELDGEDLTEAPMYVRARKGIGYLPQEPSIFRGLTVEQNILA
                     ILETQGLDARMRDDRLRELLAELHLTPLRSAPAHTLSGGERRRAEITRALVLSPRFIL
                     LDEPFAGIDPIAVSDIQNIVLHLKQRGIGVLVTDHNVRETLRMTDRAYIVHDGAIYRS
                     GTPASLAADEDVRRIYLGAEFRLD"
     gene            2365..3789
                     /gene="rpoN"
                     /locus_tag="AC16CY_15995"
     CDS             2365..3789
                     /gene="rpoN"
                     /locus_tag="AC16CY_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR3355351.1"
                     /translation="MAISQKLHTKLVQKLILTPSLQQAIKLLPMSTLELSELLNQEMV
                     ENPMLEEVPTEELQPVEAAPEKAEAKPAAEKGDAWDDQDYEYFFGDYLDDGYRPRTPT
                     EIKELPPIENTLSTGQSLADHLLWQLSMQTDEPLMREIGEAIVGNLDDDGYLVATVEE
                     LAAMGPWPLNEVERGLKLVQGFDPIGVAARDLQECLLLQLRHLGMAGSPADQIVTEHM
                     RLLQNHQAPELARKLGVSIDELKQHIELIRHLDPKPGNRYNPSQSQYVIPDVYIVKIE
                     DQYVAVLNEEGLPQLRISPVYRRLLDKNVAENTDETRAYVKDKFRSALWLIKSVEQRQ
                     KTIHKVATSIINFQRDFLDHGIEYLRPLVLRDVANDIGMHESTVSRVVTNKYMHTPQG
                     VFEMKYFFHSGISSSYGEAVSSVTIKQRIRKIIEQEDPVKPLSDSKIVSILQREGLEL
                     ARRTIAKYREELKIPTSNQRKVLY"
     gene            3828..4388
                     /gene="hpf"
                     /locus_tag="AC16CY_16000"
     CDS             3828..4388
                     /gene="hpf"
                     /locus_tag="AC16CY_16000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR3355352.1"
                     /translation="MRLELTGRHYRLTPATRALVERRVARTLRLLKDRAVSAHVVLTS
                     DRAGIHVEVTLHARRERFLHGEATGSDLELALNAAAAKIDQQARKLKGKLNGRKRRST
                     PVVSPPAEIAPPAVPLGGPAGAPPRRRIIRSTRYAVKPMSLEEAVIEMDDGRDTFIVF
                     RNSGTDTITVLFRRPDGYLGLIDPDA"
     gene            4392..5318
                     /gene="rapZ"
                     /locus_tag="AC16CY_16005"
     CDS             4392..5318
                     /gene="rapZ"
                     /locus_tag="AC16CY_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR3355353.1"
                     /translation="MAAAHASRARAARPPARKRKGPAPSRFMIVTGLSGSGKTHAIRA
                     LEDLGYFCVDNLPTQLIPTLAELCTRGQAGLGRVAIVVDVREPGFLGQFERIYRKVRA
                     LPGLEARLIFLEASRAALVRRFSETRRPHPLAPDRSVAEGIDEERRKMSAIRALADVI
                     LDTSNLTVHELREAFVRMTHVDGPRAEMVVNLLSFGFKYGAPAEADLVFDVRFLPNPH
                     FVERLRHLTGRDLKVVRFMRRSAVAREFIARLTSFLGFALPHYVSEGKSYLTVAIGCT
                     GGQHRSVMVAEALKRSLEGTAGVRLRVKHRDG"
     gene            5331..5762
                     /locus_tag="AC16CY_16010"
     CDS             5331..5762
                     /locus_tag="AC16CY_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR3355354.1"
                     /translation="MIGVVVVTHGQLASELLNAAETIVGDLPGFAAVSIGWHEDTEDA
                     RTEIEQAVARVDQGDGVLILTDMFGGTPSNLSMPFLSEGKVEVITGVNLPMLIQLVNL
                     RPRANLLTAAREAREHGRHAIWVASDLLRGEKEKPAARREP"
     gene            5759..6037
                     /locus_tag="AC16CY_16015"
     CDS             5759..6037
                     /locus_tag="AC16CY_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018175361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR3355355.1"
                     /translation="MTTRDATVVNPLGLHARAAARFVHLATQFQSQIQVARDAKVMDG
                     KSIMGILLLAASRGSTITVIADGPDEATAVDALIELVETGFGEESWSV"
     gene            6025..>7091
                     /locus_tag="AC16CY_16020"
     CDS             6025..>7091
                     /locus_tag="AC16CY_16020"
                     /inference="COORDINATES: protein motif:HMM:NF017346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HIR3355356.1"
                     /translation="MERLKGIGVSPGVAVGPALVAIQRTQVIRFPIGADRVARELDAL
                     ERARRRSREQLEQIRTRIAESRGADLAAIFDAQLLMLDDPILVGRAALVVREDRVNAE
                     WAVQRALDDAAAVFDGVNDPYLRERKGDLHDVAGRLRRNLREERGGPRDRLQDLDTPC
                     VLVADELTPSVVAQLDWTRIKGFATDAGSRTYHTAILARSLGVPAVVGLHDVSRRVPA
                     GASVIVDGETGDVTIAPTPVQAEHAAGRATQARVAVAIALDDQGPVQTLDGETVALHA
                     NIERSDDVSAALSAGAEGIGLYRSEFMLVGGPPDMAAEDEQYHVYRQLVDRMAGRPVT
                     IRTFDIDESQLARPLVDAALDA"
BASE COUNT         1264 a         2257 c         2486 g         1084 t
ORIGIN      
        1 gtcatcgtga aagacagatc ggggcggcag accatggagg ggcgctccgg cagcgcgaca
       61 atggagcgcc ttcaggacgt cgtgctcctc gattccgcgg tgcatctcct tcgcgaaggc
      121 gaagtgatcg acgccgacca tgcccgaatc acgctggcag aaggcgaaca ggtgatcacg
      181 tctctccagt tgcgtggccg ctcgagcgtc cgtggagacg gcgggcctct cgagtcgatg
      241 cgcgccgacg agatcgacct ggactatcgc gacgatggca agacggtgga agcgacggcg
      301 ctgcgtggag gagcgagcgt cacgatggcc gccgacgagc agacctcggg caggcagatg
      361 agcgggaata ctctcgatct tcagttggcc gccgacggtg cgctcacgtc gctcgagggg
      421 cggggcatgg tgtcgttcga gataccggcc gttggccgca gcccggcgcg gcgcatctac
      481 gccggcgccc tcagcgcgac cggagagccc ggcaaggggc ttgtcgcggc caggtttcgt
      541 cagaacgtgg agttccgcga gcaggggagt ggcgcgccgt ccagagaagc gcggtcccag
      601 gcgttgttcg tccagttgga cggcgatgcc gtgtcgagtg cccagttcag cgagggcgtc
      661 acgttcgacg agcaggctct gcacgccacg gcggcggaag ccacgtatca gccgggcaag
      721 agcctgctga gcctcgttgg gagcaagggc gccggcgagc ccacggtgac cgacgagcgg
      781 attcgcgttg acgcgcgatc gatcgaggtc atgctcgacg gccacgggat gatcgcccgc
      841 gacaacgtgc gcacggtgct gcgcggcagg acggcgggac ggagcgggcg cgcggcagag
      901 aacgggcggc tgccgggact cttcaaagag ggagatcccg tcagcgcgag cgcggcacgg
      961 ctggactacg aaggtcaggc aggccgggcg gtgtacacgg ggaacgcgac gcttctgcag
     1021 ggcgacacgg cgatccgagg cggcgtcgtc tcaatcgatc aggagaaggg cgatctggcc
     1081 gtgaccggct cggcccggtc aaccctggcg ctcgacggtg gcaaggctga cggacaggct
     1141 gacgaaatcc ggtacgacga ggccgcgcga caggtgacct acagcgtcac accccaggca
     1201 ccgacaaccg tggggagcgg tgtgtcggcg gcgaaggccg cggccgtgcg tccgcgcgtg
     1261 gccaggctga acggccccca gggcgacttg cgcgcgcagc ggatcgtcat cgccctggcc
     1321 cgcgacggaa acgagatcga gcggctcgag ggatacgacg acgtgacgat ggtccagggc
     1381 gagcgaacgg cggtaggcgc acgcctcacc tacgatgcac gggaggaaag ctacgtcatg
     1441 tcgggccggg gcgggacgct cgtgaccatc cgcgcgattg ttcggggctc atgccgcgaa
     1501 acggcgggag agacgctgac cttcttcaag tcgaacgaca agatcgtcgt cggcggccgc
     1561 actgaggaca aaccgtgcgt ggccgcgccg gcgcggtgac gcgctggttc gatgccgtcc
     1621 acgctgcgca cgcacgaact cacgaagtct tacagcgggc gcaccgtggt gcgcggcgtg
     1681 agcatcgagg tcgcgtctgg tgagatcgtc ggcctgctgg gcccgaacgg cgccggcaag
     1741 acgaccacgt tctacatgac cgtcggactc acgagccccg attcgggccg cgtcgagttg
     1801 gacggcgagg atctcaccga ggcgccgatg tacgtgcgcg cccggaaggg tatcgggtac
     1861 ctgccgcagg agccgtcgat ctttcgcggg ctcaccgtgg aacagaacat cctggcgatc
     1921 ctggaaacgc aggggctcga cgcccggatg cgcgacgatc ggctgcgcga actgctggcg
     1981 gagctgcacc tgacgccgct gcgatcggcg ccggcgcaca cgctgtccgg cggcgagcgg
     2041 cgccgagccg agatcactcg cgcgctcgtg ctgtccccgc gcttcatcct gctcgacgaa
     2101 cccttcgcgg ggatcgatcc gatcgcggtc agcgacatcc agaacatcgt gcttcacttg
     2161 aagcagcgcg gtatcggcgt gttggtgacc gaccacaacg tacgggagac gctgcggatg
     2221 acggaccggg cgtacattgt ccacgatggc gcaatttatc gcagtggtac ccccgcttcc
     2281 ctggccgccg acgaggatgt gcgccggatt tatctggggg cggagttccg cttggactaa
     2341 gattgatcgc gggttcctcg ggtcatggcg atttcacaga agctgcacac caagctcgtt
     2401 cagaagctga ttctgacgcc gtcgcttcag caggcgatca agctgcttcc gatgtcgacg
     2461 ctcgagttgt cggagctgct gaatcaggaa atggtcgaga acccgatgct cgaggaggtc
     2521 cccaccgaag agctgcagcc ggtcgaagcc gccccggaaa aggccgaagc gaagccggca
     2581 gccgagaagg gcgacgcctg ggacgatcag gactacgagt atttcttcgg cgactacctg
     2641 gacgacggct accggccgcg cacgccgacc gagatcaagg aactgccacc gatcgagaac
     2701 acgctgtcca cggggcagtc gctggccgat cacctcttgt ggcagctgtc gatgcagacc
     2761 gacgaaccgc tgatgcgcga gatcggcgag gccatcgtcg gcaatctcga cgacgacgga
     2821 tacctggtgg cgacggtgga ggagctcgcc gccatgggcc cgtggccgct gaacgaagtg
     2881 gagcgcgggt tgaagctggt gcagggcttc gatcccatcg gagtcgcggc ccgcgatctg
     2941 caggagtgcc tcctgctgca gctccgtcac ctggggatgg cggggtctcc ggccgaccag
     3001 atcgtcaccg agcacatgcg gctcctgcag aaccatcagg cgcccgagct ggcgcgcaag
     3061 cttggcgtct cgatcgacga actcaagcag cacatcgagc tgattcgcca tctggatccg
     3121 aagcccggga accgctataa cccgtctcag tcgcagtacg tcatcccgga cgtctacatc
     3181 gtcaagatcg aggatcagta tgtcgcggtg ctgaacgaag aggggctgcc gcagcttcgc
     3241 atcagcccgg tgtaccggag gctgctcgac aagaacgttg ccgagaacac ggacgagacc
     3301 cgtgcgtacg tgaaggacaa gttccgttcc gccctgtggc tgatcaagtc cgtcgagcag
     3361 cgccagaaga cgatccacaa ggtggcgacg agcatcatca atttccagcg cgacttcctc
     3421 gaccacggca tcgagtatct ccgcccgctg gtcctgcgag acgtggccaa cgacatcggg
     3481 atgcacgagt caaccgtcag ccgcgtcgtg acgaacaagt acatgcacac gccgcagggc
     3541 gtcttcgaga tgaagtactt cttccacagc ggcatctcga gctcctacgg cgaggcggtc
     3601 tcgtcggtga ccatcaagca gcgcatccgg aagatcatcg agcaggagga tccggtgaag
     3661 ccgctcagcg actcgaagat cgtcagcatc ctgcagcgtg aggggctcga gcttgcccgc
     3721 cggacgattg ccaaataccg ggaagagctc aagatcccga catccaatca acgcaaggtt
     3781 ctgtactgag ggccgcgtcc gggcgtgaac gcccgcacgc gccgcttatg cgtctcgaac
     3841 tgacaggacg gcactacaga ctcaccccgg caacgcgcgc gctggtcgaa cggcgggtcg
     3901 cccgcacgct tcggctgctc aaggatcggg cggtgtccgc gcacgtggtg ctcacgagcg
     3961 atcgcgcggg gattcacgtc gaggtgacgc tgcacgcgcg ccgcgagcgg ttcctgcacg
     4021 gggaggcgac gggctcggat ctcgagcttg cgctgaacgc cgccgccgcg aagatcgatc
     4081 aacaggcacg taaattgaaa ggcaagttga acggacgcaa gcgccgttca acacctgtgg
     4141 tctcgccgcc cgccgagatt gccccgccag cggtcccgct gggcggcccc gccggagctc
     4201 ctccgcgccg gcggatcatc cgcagcacgc gatacgccgt gaaaccgatg tcgctcgagg
     4261 aagccgtaat cgagatggac gatgggagag acaccttcat cgtgttccgt aactcgggga
     4321 ccgatacgat cacggtgttg ttccgccggc cggacggata cctgggcctc atcgacccgg
     4381 acgcatagac gatggcggca gcgcacgcaa gccgtgccag ggccgcccgg ccgccggcca
     4441 ggaagcggaa agggccggct ccctcgcggt tcatgatcgt gacggggctt tccgggtccg
     4501 ggaagacgca tgccatccgg gccctcgagg atctcggcta cttctgcgtt gacaatctcc
     4561 cgacgcagct catccccacg ctcgcagagc tgtgtacgcg cggccaggca gggctcggcc
     4621 gggtagcgat cgtcgttgac gtgcgtgagc ccgggttcct cggtcagttc gagcgcatct
     4681 atcgaaaggt ccgcgcgctg ccaggtctcg aggcgcggct gatcttcctc gaggccagcc
     4741 gcgcggcact ggttcgccgc ttcagcgaga cgcgcaggcc gcacccgctg gcgccggatc
     4801 gttccgtcgc ggaaggcatc gacgaggagc ggcgcaagat gtccgcgatc cgcgcgctcg
     4861 cagacgtgat cctggacacg tcgaatttga ccgttcacga gctgcgcgag gcgttcgtga
     4921 ggatgacgca cgtggacggg ccccgtgccg agatggtggt caacctcctc agcttcgggt
     4981 tcaagtacgg agccccggcc gaagccgatc tggtgttcga cgtccggttt ctcccgaacc
     5041 cgcatttcgt cgagcggctc cgtcatctca cgggccgcga tctcaaggtg gtgcgcttca
     5101 tgcgccgctc agcggtcgcg cgcgagttca tcgcacgtct cacgtcgttt ctgggctttg
     5161 ctcttccgca ctatgtcagc gagggcaaga gctatctgac cgtggccatc ggctgtaccg
     5221 gcgggcagca ccgatccgtg atggttgccg aagcgctcaa gcgatcgctc gaagggaccg
     5281 cgggcgtgcg gcttcgggtg aagcatcgcg acggctgacg cgggcgtgtc atgataggcg
     5341 tcgtcgttgt cacgcacgga caactggcct cggagctcct caacgccgcc gagaccatcg
     5401 tgggcgacct cccgggcttc gcggcggtgt ccatcggctg gcacgaagac accgaggacg
     5461 cccggaccga gatcgagcag gcagtcgcgc gagtggacca gggcgacggc gtgctcattc
     5521 tcacggacat gttcggagga acgccgtcga acctgtcgat gccgttcctg tcggaaggga
     5581 aggtggaggt catcacggga gtgaacctgc ccatgttgat ccagctcgtg aacctgcggc
     5641 cgcgcgcgaa cctgctgacg gccgcgcgtg aggcccgcga gcacgggcgc catgcgatct
     5701 gggttgcctc cgatctgctg cgaggggaaa aggagaagcc cgcggcgcgg cgcgagccat
     5761 gacgacacgc gacgccacgg tcgtgaatcc gctcgggctg cacgcgcgcg ccgcagcgag
     5821 attcgtgcac ctggccacgc agttccagtc gcagattcag gtggcccggg acgcaaaggt
     5881 gatggacggc aagagcatca tgggcatcct gctgctggcc gcgtcccgcg ggtccaccat
     5941 caccgtgatc gcggacggcc cggatgaagc caccgccgtg gacgccctga tagaactggt
     6001 cgaaaccgga ttcggcgagg aatcgtggag cgtctgaagg gcatcggcgt ctcgccagga
     6061 gtggcggtgg gcccggcgct ggtcgccatc cagcggacgc aggtcatccg gttcccgatc
     6121 ggcgctgatc gggtcgcgcg cgagctggac gcgctcgagc gggcacgacg gcggtctcgt
     6181 gagcagctcg agcagatccg cacccgtata gccgagagcc gcggcgccga tctcgccgcc
     6241 atcttcgacg cccagctgct gatgctcgac gaccctattc tggttggacg agcggccctc
     6301 gtcgtgcgcg aggatcgggt caacgccgag tgggccgtcc aacgcgcgct ggatgacgcc
     6361 gccgcggtgt tcgacggcgt gaacgacccg tacttgcgcg agcggaaggg tgacctgcac
     6421 gacgtggccg gacggctgcg gcggaatttg cgagaagaga gaggcgggcc ccgggatcgg
     6481 ctgcaggacc tcgatacgcc gtgcgtactc gtggcagacg agctgacgcc ttccgtcgtc
     6541 gctcagctcg actggacgcg gatcaagggg ttcgcgaccg acgcgggcag ccggacgtat
     6601 cacaccgcca tcctcgcgag atcgctgggc gtaccggcgg tcgtggggct tcacgatgtc
     6661 agtcggcgcg tgcccgcggg agcctcggtg atcgtcgacg gcgaaacggg agacgtgacg
     6721 atcgcgccga cgccggtaca ggccgaacac gccgcgggcc gtgcgacaca ggcgagggtg
     6781 gcggtcgcca tcgcgctcga cgaccagggt cctgttcaga cgctggacgg cgagaccgtg
     6841 gcgctgcacg ccaacatcga gcgatccgac gacgtgtcag ccgcgctgag cgccggggcc
     6901 gagggtatcg ggctgtatcg ctcggagttc atgctcgtcg gggggccgcc cgacatggcg
     6961 gccgaggacg agcagtacca cgtgtaccgg cagctcgtag accgcatggc gggcaggccg
     7021 gtcacgatcc ggaccttcga catcgacgag agccagctcg cgcggccgct cgtggacgcg
     7081 gcgctcgacg c
//
LOCUS       DBNGSH010000696         5598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_74374, whole genome shotgun sequence.
ACCESSION   DBNGSH010000696 DBNGSH010000000
VERSION     DBNGSH010000696.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_74374"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="AC16CY_16025"
     CDS             complement(<1..898)
                     /locus_tag="AC16CY_16025"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3355357.1"
                     /translation="MPPVSADLHVVFGAGQVGFPLAARLRAAGKRVRIAKRSSGDLPA
                     GCEVALGDAADPAFCDEAARGAAVVYHCMNPPYDARIWADLIPRYMGNLIDASARTGA
                     RLVVLDNVYMLGRPRGLPLNEDTPVNPTSRKGEIRARAAERLFDAHRKGDVVAAAGRA
                     SDFYGPRGTQTALGDFFWPRALAGKTIYSPFSLDAVHTYHYIPDVASGLATLGCADVD
                     AYGRPWMLPCVPAGTLREIVGRIAMKLGREIRLGQLPRRLLRVMALFMPLFREIDEML
                     YQWEEPFVIDDRQFRERFHVVPT"
     gene            complement(918..2036)
                     /locus_tag="AC16CY_16030"
     CDS             complement(918..2036)
                     /locus_tag="AC16CY_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3355358.1"
                     /translation="MSTRCHVATRKGLFTFERGRSGWSVTRVSFLGDNCTLAMHDPRT
                     GSLYAALNHGHFGVKVHRSNDGGETWQEVGTPKYPEKPADYVPKKTPAEGVAVDWALK
                     LVWALAPGGADEKGVLWCGTLPGGLFRSADGGDSWELNRPLWDDPKREEWFGGGADQP
                     GIHSICVDPRDPRHLTIAVSCGGVWSTRDGGGSWQLGGRGMRAAYMPPERQFDPAVQD
                     PHILAQCPANPDVMWVQHHNGIFRSTDAGASWLEIENVAPSTFGFAVAAHPSDSDTAW
                     FVPAQKDERRIPVDGRVVVTRTRDGGRTFDTLTKGLPQQHAYDLVFRHALDVDEAGER
                     LAFGSTTGSLWLSENAGDAWQALSSNLPPIHAVRFEKS"
     gene            complement(2043..2312)
                     /locus_tag="AC16CY_16035"
     CDS             complement(2043..2312)
                     /locus_tag="AC16CY_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3355359.1"
                     /translation="MATVRFAQSIQRHVECHPREVAGDTIGAVLDRYFTENERARGYV
                     LDDQGRLRQHMAIFVDGLHIRDRKGLTDPVAPDAVVDLIQALSGG"
     gene            2443..2856
                     /locus_tag="AC16CY_16040"
     CDS             2443..2856
                     /locus_tag="AC16CY_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3355360.1"
                     /translation="MAGHTHGEFACPIETTLEVLGGKWKGMVLHRLIFGTRRFNELRR
                     LLPHVTQRMLTRQLRELERDGVIRRRVYAEVPPRVEYSLSEFGLSLKPILLMMGDWGA
                     AYQDKVRKMKMSAPAPGTGGRLAAAASQQATANRT"
     gene            complement(2933..>5598)
                     /locus_tag="AC16CY_16045"
     CDS             complement(2933..>5598)
                     /locus_tag="AC16CY_16045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3355361.1"
                     /translation="ELRLGPAREAEIIEELSQHLDDRYDELRASGSSDAEARRLVMAE
                     LAEGGGLEERMHALSQAHVTPPVVHGQPAAGLLRGLRQDVQYAVRTVRRQPGFAATIV
                     VTIGLGIAVNTMVFTIANAAVLRPLPFEEAERLVRLGVTNAGNARNPTDGISYPDFLD
                     WQAARQTFEQVAAISDRDADISDDEHPAARVEAAFASWNAFSLLRQPPALGRDFTEAD
                     DRIGAPPVVILSDNLWRVRYGADPAILGKSIRVAGTPSTIVGVMPGDFGFPNRVEIWL
                     PLAALSQSERTSRDARRLDGLGRLRSGASIEQATTELSGITASLGERHPDTNRNITPL
                     VTSMAIATQFVAAVMALLAAVGFVLLIACANVANLLLARAADRSRDVTLRLALGASRW
                     RIVRQLLVESLLLALLGGLAGLALAYPGIQLFQNLPPESAPPYWVRFTLDRTVFAYLL
                     MLCVGSAIVCGLVPAWHASRTDLVATLNDAGRGSAGSRQRRRWTGAFVVAQVALAVVL
                     LTGASLMMQNLIGLVRVDAGVETDGLFRTGFDLRRSDYHPERRLQFFGQLEERLGSSP
                     DVRAALATHAPMGGGFIRRLRIEGTPASDVNTLPIVSLVRVGRRYFDVVGARAIAGRS
                     FADSEGRLPDDGVVVNERFARMQFQNEPAIGKRILLLELNEAAGAADDPQWLTIVGVV
                     GNVRQSMLPSGEFDAVVYGPYTSDPPQGIQVLMRSAAGRAAASALVSDHVRVLDRDLP
                     VLPVRTTDEAMAIQFWPQRIFGSLFAVLASVAMLLATCGLYAVTSYAVSRRTREIGVR
                     VALGADARSIWWAVTGATLRQLVIGLVLGMAGAALVTTALPAILVGTGGVRLFAFAGA
                     VIALVAAGLAASALPARRAVRIDPVAALQSE"
BASE COUNT          978 a         1890 c         1878 g          852 t
ORIGIN      
        1 ctgtcgggac gacatggaag cgctcgcgga actgtcgatc gtcgatgacg aacggctcct
       61 cccattgata gagcatctcg tcgatctccc ggaacagcgg catgaacagg gccatgaccc
      121 tgaggagccg gcggggcagc tgacccaggc gaatctcgcg ccccagcttc atggcgatcc
      181 ggccgacgat ctctcgcagc gtgcctgcgg gcacacaagg caacatccag gggcggccat
      241 aggcatcgac gtccgcgcaa ccaagcgtgg ccaggccgga ggccacgtca gggatgtagt
      301 gatacgtgtg gacggcgtcg agcgaaaacg gcgagtagat ggtcttgccc gcaagcgctc
      361 gaggccagaa gaaatcgccc agcgcggttt gcgttcctcg aggtccgtag aaatccgacg
      421 cccgtcccgc ggccgccacg acgtctccct ttctgtgggc gtcgaacagg cgttcggcgg
      481 cgcgcgcgcg gatctcgccc ttgcggctgg ttggattcac cggcgtgtcc tcgttgagcg
      541 gcaggccgcg cggacgcccc agcatgtaga cattgtcgag gacgaccagg cgggccccgg
      601 tacgggccga cgcgtcgatc aggttgccca tgtagcgcgg gatcaaatcc gcccagatgc
      661 gcgcgtcgta cggcgggttc atgcagtgat acaccactgc cgccccgcgg gcggcctcgt
      721 cgcagaaggc gggatccgct gcgtcgccga gcgcgacctc acaacccgcg ggaagatcgc
      781 cggaggagcg ttttgcgatt cgcacgcgtt tgccggccgc acgcaggcgc gcggcaagag
      841 gaaatccgac ctgtcccgcg ccgaagacga cgtgcaggtc cgcactcacg ggtggcatcg
      901 gggctcctga tccgcgctca gctcttctcg aaccgcaccg cgtgaatcgg tggcagattg
      961 gatgagagcg cctgccacgc gtcgccggcg ttctcgctca gccagagcga gccggtcgtc
     1021 gagccgaacg ccagccgctc gcccgcctcg tccacatcga gcgcgtgccg gaacaccagg
     1081 tcgtacgcgt gctgctgcgg cagccccttc gtcagcgtgt cgaacgtgcg tccgccatcc
     1141 cgtgtgcggg tgaccaccac gcggccgtcc acgggaatcc gcctctcgtc tttctgcgcc
     1201 ggaacgaacc aggccgtgtc cgaatcggac gggtgagccg ccacggcgaa accgaacgtc
     1261 gacggcgcga cgttctcgat ctcgagccac gacgcgcccg cgtcggtcga gcggaagatg
     1321 ccgttgtgat gctgcaccca catgacgtcc ggattcgccg ggcactgcgc gaggatgtgc
     1381 ggatcctgga cggcggggtc gaactgtcgc tccggcggca tgtaggcggc gcgcatgccg
     1441 cggccgccga gctgccagga gccgccgccg tcgcgggtgc tccagactcc gccgcacgac
     1501 acggcaatcg tgaggtggcg cggatcgcgt gggtccacgc agatggagtg gatgcccggc
     1561 tgatcggcac cgccgccgaa ccattcctcg cgcttcgggt cgtcccagag gggacggttg
     1621 agctcccagg agtccccgcc gtcggccgat cgaaagaggc cccctggcag cgtcccgcac
     1681 cagagcacgc ctttctcgtc ggccccgccc ggtgccagcg cccacacgag tttgagcgcc
     1741 cagtcgaccg ccaccccttc ggcaggcgtt ttcttcggca cgtagtccgc cggcttttcg
     1801 gggtacttcg gcgtgccgac ctcctgccac gtttcaccac cgtcgttcga tcggtgcacc
     1861 ttgacgccga agtgtccgtg attgagcgcg gcgtacaacg acccggtgcg cggatcgtgc
     1921 atcgccagcg tgcagttgtc gccaagaaaa ctcacgcgcg tcacggacca gccggaccgg
     1981 ccgcgctcga atgtgaagag gcccttgcga gtggccacat gacaccgcgt gctcatgggt
     2041 ggttatcctc ctgagagtgc ctggatcaga tcgacgacgg catcgggtgc aaccgggtcg
     2101 gtcaacccct tgcgatcgcg gatgtggagt ccgtcgacga agatcgccat gtgctgacgg
     2161 aggcgtccct ggtcgtccag cacgtaaccg cgtgcccgtt cgttctccgt gaagtagcga
     2221 tcgagcacag caccgatcgt gtcgccggcg acctcgcggg ggtggcactc gacgtgccgc
     2281 tggatgctct gggcgaatct cacggtggcc atctcgatcc ctgcctccca acccctgatt
     2341 cgaggcggcc agtatgggct ctatgtcctc gagacgaaag tacgcacatt ttcggcatat
     2401 agtgtcgaaa ctgataccta cccttgacgt ggagcgtgca cgatggctgg acacacgcat
     2461 ggagagttcg cctgcccgat tgagaccact cttgaagtcc tgggaggcaa gtggaaaggg
     2521 atggtcctcc acaggctcat cttcgggaca cggcgattca acgagctgcg tcgtttgctt
     2581 ccccacgtga cgcagcggat gctgacgcgc cagttgcgcg agctcgagcg cgatggcgtg
     2641 attcgacgcc gggtctacgc ggaagtgccg ccgcgcgtcg agtacagcct cagcgagttc
     2701 ggcctgagcc tcaagccgat cctcctgatg atgggggact ggggcgctgc atatcaggac
     2761 aaggtgcgaa agatgaagat gagcgcgccg gcgcctggaa cgggcgggag gcttgccgcc
     2821 gctgccagcc agcaggcaac ggcgaaccga acctgaacac gaacagagaa gcgagaactg
     2881 agaagagtga acgacagatc tctctgctcg attcaggtca ccctgggatc agtcactcgc
     2941 tctgcagcgc cgccaccggg tcgatccgca ccgcgcggcg cgcaggcagc gcgctcgccg
     3001 ccaggccggc ggccaccagc gcgatgacgg cgccggcaaa tgcgaacagg cgcacacctc
     3061 cggttccgac aagaattgcc ggcagggcgg tcgtgaccaa cgcagcaccg gccatgccga
     3121 ggacgagccc gatgacgagt tgacgcaacg tcgcacccgt gaccgcccac cagatgcttc
     3181 gggcgtcggc tccgagcgcc acgcgcacgc cgatctcccg cgttctgcgc gagaccgcat
     3241 aagacgtgac ggcgtacagg ccgcacgtgg ccagcagcat cgcaaccgat gccagtacgg
     3301 cgaacagcga gccgaatatc cgttgaggcc agaactgaat cgccatggcc tcgtctgtcg
     3361 tccggaccgg taacacgggc agatcacgat cgagcacacg aacgtgatcg ctcacgagcg
     3421 cgctggccgc tgcccggccc gcggccgagc gcatcagcac ctgtatcccc tgtggcggat
     3481 ccgacgtgta ggggccgtag acgaccgcat cgaactcgcc ggacggcagc atcgactggc
     3541 gcacgttccc caccacgccg acgatggtca gccattgggg atcgtcggcg gcccccgcgg
     3601 cctcgttcag ctccagcaga aggatgcgct tgccgatcgc cggttcgttc tgaaactgca
     3661 tccgcgcgaa tcgttcgttg acgacaacgc cgtcgtccgg gaggcggccc tcactgtcgg
     3721 cgaacgagcg accagcgatc gcgcgcgcgc ccacaacgtc gaagtagcgc cggccgacgc
     3781 ggacgagcga gacgatcggg agagtgttga cgtcagaagc cggcgtcccc tcgatgcgga
     3841 gcctccggat gaaccctcct cccatcggcg catgggtcgc cagcgcggca cggacgtccg
     3901 ggctcgagcc gaggcgctcc tccagctggc cgaagaactg aagacgccgc tcggggtgat
     3961 agtcgcttcg gcggagatcg aaccccgttc gaaacagccc gtcggtctcg acgccggcgt
     4021 cgacccgcac caggccgatc aggttctgca tcatcagcga ggctccggtg agcagtacga
     4081 ccgccagcgc cacctgcgcg accacgaagg cgccggtcca ccggcggcgc tgccggctgc
     4141 cggcgctgcc gcgccccgca tcgttcagcg tggcgacgag gtccgttcga gacgcgtgcc
     4201 acgccggaac caggccgcac acgatcgcgc ttcccacgca gagcatgagg aggtaggcga
     4261 agaccgtccg atccagcgtg aaccggaccc aatacggggg cgcggattct ggcgggaggt
     4321 tctggaagag ctgtattcca ggatacgcga gcgcgagtcc ggccagaccg cccagcaatg
     4381 ccagcagcag gctctcgacg agcagttgac ggacgatgcg ccagcggctg gcgcccaggg
     4441 ccaggcgcag cgtgacgtcg cgcgaccgat cggccgcacg cgccagcagc aggttcgcga
     4501 cgttggcgca cgcgatgagc agtacgaatc cgacggcggc gagcaaggcc atgaccgcgg
     4561 cgacaaactg cgtggcgatc gccattgacg tgacaagggg cgtgatgttc cggttcgtgt
     4621 ctggatggcg ctctcccagt gaggcggtga taccggacaa ctcggtcgtg gcctgttcga
     4681 tcgatgcgcc ggaacgcagg cgtccgagac cgtccagccg acgcgcgtct cgcgacgtcc
     4741 tctccgactg cgacagtgcc gcgagtggca gccagatctc cacgcgattg ggaaagccga
     4801 aatcccccgg catgacgccg acaatggttg acggcgtgcc ggccacacgg atcgacttgc
     4861 cgagaatcgc cgggtcggcg ccataacgca cgcgccagag gttgtcgctc aggatgacga
     4921 ccggcggcgc gccgattcga tcgtcggctt cggtgaaatc gcggccgagc gcgggcggct
     4981 ggcggagcag cgagaacgca ttccacgacg cgaacgcagc ctcgacgcgg gcagccgggt
     5041 gctcgtcgtc cgagatgtcc gcgtcgcgat ccgaaatcgc cgcgacctgc tcgaacgtct
     5101 gccgggcggc ctgccagtcc aggaaatccg gataagaaat gccgtcggtc gggtttcgtg
     5161 cgttgccggc gttggtcacg ccgagccgga caagccgctc cgcctcttcg aacgggagcg
     5221 ggcggagcac tgccgcgttg gcgatcgtga agaccatggt gttgacggcg atgcccagcc
     5281 cgatcgtgac gacgatcgtg gcggcaaacc ctggctgcct gcgtaccgtg cgcactgcgt
     5341 actgcacgtc ctgcctgagg ccgcgcagca gacccgcggc cggctgtcca tgaacgacgg
     5401 gcggtgtcac gtgcgcctgc gagagggcgt gcattcgctc ctccagtccg cccccttcag
     5461 cgagctccgc catgacgagc cgccgcgcct cggcgtcgct cgagcccgag gcccgaagct
     5521 cgtcgtagcg atcgtcgaga tgctgcgaca gctcttcgat gatttccgcc tcgcgggccg
     5581 ggccgaggcg cagttcgg
//
LOCUS       DBNGSH010000697         4499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3131493, whole genome shotgun sequence.
ACCESSION   DBNGSH010000697 DBNGSH010000000
VERSION     DBNGSH010000697.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3131493"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="AC16CY_16050"
     CDS             <1..1079
                     /locus_tag="AC16CY_16050"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3355362.1"
                     /translation="ADPVTPAYVRQARFVLGMLGPGSLRPALGASPDFAGDLGGLKGA
                     DVLLVFVESYGAVTYETSAIADGLVAGRADLAAAVADTGREVVTAYVDSPTFGGSSWL
                     AHLSLISGVEVRDQYAYTSLMASGRETMITNFARRGYRTVALMPGMRQAWPEGAFYRF
                     DQIYGHDLLEYKGPQFGWWSIPDQYALAKIDAVERARAAEARSPLFIVFPTSTTHAPF
                     GPVAPYQPDWARVLTAEPFEPADAERALAATPNLIDLRPSYVRAMAYEYTTIAGYLRE
                     HARDDMVLIAIGDHQPPAAVSGRGASWSVPVHVFGRRGQVIDRLIDQRFRPGLEPLRP
                     SIGPMHALVPMLLQAFGTIGSSDN"
     gene            1201..4296
                     /locus_tag="AC16CY_16055"
     CDS             1201..4296
                     /locus_tag="AC16CY_16055"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3355363.1"
                     /translation="MRTRLRKFLLVLVVLALPAAASAQGLGAVAGAVRDTSGAVLPGV
                     TVEASSPALIEKVRSGVTDALGQYRIVNLPPGTYVITFTLTGFGTVRRDGIEVALNVT
                     SNVNADLRVGAVQETITVSGESPIVDVQSSAQTRTVSAEAFKELPVGSSWIQMASLVP
                     AIQASNVDVGGILGDQTGAQVRAHGSAYMDGVSMIDGLRIGNMYLSSNLTNMSLSPLL
                     FDQVDISLAGQIAETGTNGVIMNAVPKAGGNVFSGSVLGNFSDPALQGDNVTERLNNR
                     GLTNASTTLKTLYDINGAIGGPIKQDKVWFYFTSRYFTNYYYLASRFYPVDVTAVNRT
                     NDTNRQAYGGTYTADNNGRVTWAINDSQKISGWYAYQYKVDPHWLIQINTASPEGIRI
                     TRWHTQLSTTKWTYTATNRLLFEAGLMAGESPDTIRMDPEQVGQCQNQTNAFQGTLAP
                     VCIAIVNQSAGNFFYRAPTGFDFDDRLPSQSFNVASSYVTGSHNVKVGFEMQRGHFLR
                     GDNNDSTGGIWYSILNNQPSLVTIQSPLSGWQNNLNYNLGIFAQDRWTANRLTVSAGV
                     RLDFLNESTEPFTATPHRWLPNRNNSFGAVENVPNWKDVNPRLSAAYDLFGNGKTALK
                     ASASRGVEQDSIRYAAANNPATTLVTQAQRTWNDANGNFNPDCDLMSGTEQDLRPTGG
                     DLCGAWVPSSFGSSVPGLSYSRAVMEGWGVRPYNWEYSAGIQQEIVPRLSASFAYFRR
                     AYGNFYVVDNEALAANQYTEFSVTVPSNPAFPTLPNAGQTVNGLYDPIANVAPRNVVK
                     DASQFGDQVQTWDGIDLTVDARLRNGLFLQGGVSTGRTITDNCEIVDDVPESLQSGAV
                     GLLPPAGIQPPMGLLPFPFFVGSWTPRDYCRQQSPYLAQYKAIASYTLPWWGVRLSGT
                     FQSIPGPIVGANNVYANPAAGGPPLGTSIGRPLINGQANVQLIEPGSMYGDRLNQFDL
                     RFTKILDVGKGRLDLNVDIYNAFNSDAVTLELPGYGPVWRLPLTVIQPQFIKFAARWD
                     F"
BASE COUNT          821 a         1489 c         1441 g          748 t
ORIGIN      
        1 tcgccgatcc agtgacgccg gcgtatgtca ggcaggcccg attcgtgctc gggatgctcg
       61 gccccggatc gctgcggccg gcgcttggcg ccagcccgga cttcgcgggc gatctcggcg
      121 gcctgaaagg cgccgatgtc ctgctcgtct tcgtggagtc ctacggagcg gtcacgtacg
      181 aaacgtcggc catcgccgac ggtctcgtcg cgggccgcgc cgatctcgcg gcggcggtgg
      241 cggacacggg ccgcgaggtc gtgaccgcgt acgtcgactc tcccaccttc ggcgggtcgt
      301 cgtggctggc gcacctgagc ctcatctcgg gcgtggaggt gcgcgatcag tacgcctaca
      361 cgtcgctgat ggcgtcggga cgcgagacga tgatcaccaa cttcgcgcgc cgcggttacc
      421 ggaccgtggc gttgatgccg gggatgcgtc aggcctggcc ggagggggcg ttttatcgct
      481 tcgaccagat ctacggccac gacctgcttg aatacaaggg tccgcagttc ggctggtgga
      541 gcatcccgga tcagtacgcg ctcgccaaga tcgacgccgt cgagagagcg cgcgcagccg
      601 aggcccggtc gccgctcttc atcgtctttc ccacgagcac gacgcacgcg ccgttcgggc
      661 cggtcgctcc gtatcagccc gactgggcgc gcgtgctgac tgccgaaccc ttcgagcctg
      721 ccgacgccga gcgagccttg gcagccacgc cgaacctgat cgatctgcgg ccaagttacg
      781 tgcgggcgat ggcctacgag tacacgacga tcgccgggta ccttcgagag cacgcacggg
      841 acgacatggt tctcatcgcc atcggcgacc accagccgcc ggcggccgtg agcggtcggg
      901 gcgcctcatg gagcgttccc gtgcacgtgt tcgggcggcg tggccaggtg atcgatcgac
      961 tgatcgatca gaggtttcgt cccggcctcg agcctcttcg cccctcgatt ggtcccatgc
     1021 acgccctcgt cccgatgctc ctccaggcct ttggcacgat cggatcgtcg gacaactgag
     1081 ccggcgaggc gccgccgcta ccttgacacc acggacggcg aagcgtatat gtttttccgc
     1141 caaaggaaaa cacattcgca tagcgaaagc cttaaaagtc tttcagcatc cggaggagac
     1201 atgcgaacgc gactgagaaa gttcttgttg gtcctcgtcg tgctggcgtt gccggccgcg
     1261 gcgtccgcac aggggctcgg agccgtggcg ggcgccgtca gggatacttc cggcgccgtg
     1321 ttgcctggcg tgacggtcga agcgtcgagt cccgccctga tcgagaaagt gcgaagcggt
     1381 gtgacggacg cgttgggcca gtaccggatc gtgaacctcc cgccgggcac atacgtgatc
     1441 accttcacgc tgacgggatt cggcacggtc cgacgcgatg gcatcgaagt ggccttgaac
     1501 gtcacgtcca acgtgaacgc cgatctgcgc gtcggcgccg tgcaggagac gatcaccgtg
     1561 tcgggggaga gcccgatcgt ggacgtgcag tcgtccgcgc agacgagaac cgtctcggcc
     1621 gaggcgttca aggagctccc cgtcggcagc tcctggatac agatggcatc gctcgtcccg
     1681 gccatccagg cctcgaacgt ggacgtcggg ggcatcctgg gcgaccagac cggcgctcag
     1741 gtcagggcgc acggcagcgc atacatggac ggcgtgtcga tgatcgacgg cctgcgcatc
     1801 ggcaacatgt acctcagctc gaacctgacg aacatgagcc tgtcgccgct gctgttcgac
     1861 caggtggaca tctcgttggc cggccagatt gccgagacgg gcaccaacgg cgtcatcatg
     1921 aacgctgtcc cgaaagccgg agggaacgtc ttcagcgggt cggtgctcgg caatttctcg
     1981 gatcccgcct tgcagggcga caacgtcacc gagcggttga acaaccgcgg gctgacgaac
     2041 gcctcgacca cgctcaagac gctctacgac atcaacggcg cgatcggcgg acccatcaag
     2101 caggacaagg tgtggttcta cttcacgtcg cgctacttca ccaactatta ctacctggct
     2161 tcgcgcttct acccggttga cgtgacggcg gtcaaccgca ccaacgacac gaaccgtcag
     2221 gcctatggcg gaacgtatac ggccgacaac aacgggcggg tgacctgggc gatcaacgac
     2281 agccagaaga tctcgggctg gtacgcctac cagtacaaag tggacccgca ctggctgatc
     2341 cagatcaaca ccgcctcgcc cgaggggatt cgcatcacca ggtggcacac ccagctgtcc
     2401 accaccaagt ggacctacac ggcaaccaac cgcctgctgt tcgaggccgg attgatggcc
     2461 ggcgagagcc cggacaccat caggatggat cccgagcagg tcgggcagtg ccagaaccag
     2521 acgaacgcgt tccagggaac gctggcgccg gtgtgcatcg cgatcgtcaa ccagagcgca
     2581 ggcaacttct tctaccgtgc gcccaccggc ttcgacttcg acgaccggct gccgtcgcag
     2641 tccttcaacg tggcatcgag ctacgtgacc ggctcgcaca acgtcaaggt cggtttcgag
     2701 atgcagcggg gccacttcct gcgcggagac aacaacgact cgaccggcgg catctggtac
     2761 agcattctca acaaccagcc gagcctcgtc acgatccaat cgccgctgtc ggggtggcag
     2821 aacaacctga actacaacct cggcatcttc gcgcaggacc gctggacggc aaaccgtctg
     2881 acggtgagcg cgggcgttcg acttgacttc ctcaacgagt cgaccgagcc gttcacggcg
     2941 acgccacacc ggtggctgcc caaccggaac aacagcttcg gcgccgtcga gaacgtcccg
     3001 aactggaagg acgtcaatcc ccgtctctcc gccgcatacg acctgttcgg caacgggaag
     3061 accgcgctca aggcgagcgc cagccgcggc gtcgaacagg actcgatccg ctacgcggcc
     3121 gccaacaacc cggcgacgac cctcgtcacg caggcgcagc ggacgtggaa cgacgccaac
     3181 gggaatttca acccggactg cgacctgatg agcggtacgg agcaggactt gcggcccact
     3241 ggcggggacc tctgcggcgc ctgggttcca tccagtttcg gcagcagcgt gcccgggctg
     3301 tcgtactcca gggcggtcat ggaagggtgg ggcgtgcggc cctacaactg ggagtattcg
     3361 gcgggcatac aacaggaaat cgtgcctcga ctgtccgcct cctttgcgta cttccgccgc
     3421 gcctacggga acttctacgt ggttgacaac gaggcgcttg cggctaacca gtacaccgaa
     3481 ttctccgtga cggtcccgag caatcctgcc tttcccacgc ttcccaacgc gggccagacc
     3541 gtcaatggcc tctacgatcc gatcgccaac gtggccccgc ggaacgtcgt caaggacgcg
     3601 tcgcagttcg gagaccaggt ccagacctgg gacgggatcg acctgacggt cgatgcccgg
     3661 cttcgcaacg gcctgttcct tcagggcggc gtcagcacgg gcaggacgat caccgacaac
     3721 tgcgaaatcg tcgatgacgt gccggagtcg ctccagtccg gcgcggtggg cctcctgcct
     3781 ccagccggca tccagcctcc catgggcctg ctcccgttcc cgttcttcgt cggcagctgg
     3841 acaccgaggg actactgccg tcagcagtcg ccgtacctgg ctcagtacaa ggcgattgcg
     3901 tcctacacgc tgccgtggtg gggcgtccgt ctcagcggaa cgttccagag cattcctggt
     3961 ccgatcgtgg gcgccaataa cgtctacgcc aaccctgctg ccggtgggcc cccgctgggt
     4021 acatccatcg gcaggccgct gataaacggg caggccaacg tgcagctgat cgagccgggc
     4081 tcgatgtacg gcgaccggct caaccagttc gacctgcgct tcacgaagat cctcgacgtg
     4141 ggcaagggcc ggctcgatct gaacgtggac atctacaacg cgttcaactc ggatgcggtc
     4201 accctggagc tccccggcta cgggccggtg tggcggttgc cgctgacggt gattcagccg
     4261 cagttcatca agttcgcggc gaggtgggat ttctagggat cagggatcag ggcccagggc
     4321 ccagggctgg gcaaggcctg agacggccct gaggcctggg ccctgattcc tagttacttc
     4381 gacacactga agggcagccg cgccccgccg acagccaatg caagcaggcc gcccaggacc
     4441 ggcgggccgt acgcattgga cagatagctg tagtgaagga gcacgtcggc ggcgaagtg
//
LOCUS       DBNGSH010000698         4111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4150813, whole genome shotgun sequence.
ACCESSION   DBNGSH010000698 DBNGSH010000000
VERSION     DBNGSH010000698.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4150813"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="AC16CY_16060"
     CDS             complement(<1..999)
                     /locus_tag="AC16CY_16060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3355364.1"
                     /translation="MRETLEVDILIVGGGPAGLSAALRLAQLQKGRSEPLSIAVIEKA
                     RDAGAHSLSGAVLDPSALRQLVPDFDIGAAAFASPVRRDQVYFLTGKSRLRFPIVPPP
                     LQNHGHVIVSLNALVRWLSERVEAEGVDVFSGFGGREVLFDGTRVIGVRTGDRGTARD
                     GTRKPTFEWGVDILAKITIFCEGVRGNLTKDVIGTLALDRGREPAQFAVGLKELWEVP
                     AKRLEAGTVIHTMGYPLRHDEFGGGFMYAMPEGRLSVGFVVGLDYHDPLFDPHMAFNR
                     FKQHPFVSGVLDGGTMVRYGAKALPEGGWNTIPRPYMDGGLIAGDAGGYLNSMRLKG"
     gene            complement(1015..1980)
                     /locus_tag="AC16CY_16065"
     CDS             complement(1015..1980)
                     /locus_tag="AC16CY_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3355365.1"
                     /translation="MILVVAEQRAGALNRASWEAVAAAQQLAGGMPVKAVVFGEATDN
                     AASELASAGLDVLAVDHPALAGYTPDGFTQALQQVVAAVSPSYVLLPHTYQTRDFAPM
                     AAVRMGAPLVTDVTGIRGSGSSATFVRPVFQGKLAAEVRHEGNGPRLVTFQIGAFRAD
                     AVQRGDRPGGVTRMEVSIDAATIRQTPEPPFKEARQAVDLGQAERIVAVGRGIKSQEN
                     IAVAEKLARAMSAEVAASRPICDSGWLPMERQIGSSGQTVAPKLYVALGISGAIQHLV
                     GMKGSRTIVAINKDADAPIFEVADYGIVGDLFEIVPALITELDKQ"
     gene            complement(1977..2759)
                     /locus_tag="AC16CY_16070"
     CDS             complement(1977..2759)
                     /locus_tag="AC16CY_16070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3355366.1"
                     /translation="MKIAVCIKQVPTREWQPRLREDKAWIREQDASFELNEPDAFALE
                     EALRLREKHSGEVVACAAGPARVQQVIREALARGADRALHVDDEALASADASITSAAL
                     ASAMADEHFDLVLTGLQSDDQGHAQVGVMLAERLGVPHSTIIMEVQIVPSSGPGQAAR
                     LRVKRELEGGWFQWIGMPLPAVLTIQSGINQLRYATLKGIMAAKKKEIRRVPLPPGLA
                     ASQRILGLSLPARSKQTKMLTGSAADIARELARLLRDEARAL"
     gene            complement(2797..4038)
                     /gene="fabF"
                     /locus_tag="AC16CY_16075"
     CDS             complement(2797..4038)
                     /gene="fabF"
                     /locus_tag="AC16CY_16075"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3355367.1"
                     /translation="MSRRVVITGIGLVSSLGIGTDATWKALVAGTSGVDRISKFDVTG
                     FATQIAAEVRGFDALDYIDKKDVKKMDVFIQYAVAASQFAMDDSRLEITPSNAPDIGV
                     FIGSGIGGFQTIEREHSALLNGGPRRISPFFIPSAIINLAAGQVSIRFGAKGPNLATC
                     TACSASAHAIGESFEIIRRGDAEAMIAGGSEAAITPMSVGGFGALRALSTRNDNPRGA
                     SRPFDKDRDGFIIGEGAGMLILEELDFARKRGAHIYAEIVGYGMSGDAYHITAPSEDG
                     DGGVRVMKKAVCKAGIPPEDVDYINAHGTSTPYNDRLETLAIKKLFGDHAYKLAVSST
                     KSMTGHLLGGAGGLEAGISALAVHHQVAPPTINLDDPDPECDLDYVPHTGRPMSIRYA
                     LSNSFGFGGTNAALLFKKFDG"
BASE COUNT          666 a         1434 c         1315 g          696 t
ORIGIN      
        1 gcccttgaga cgcatcgagt tcagataccc gcccgcgtcc ccggcgatga ggcctccatc
       61 catgtacggc cgtggaatcg tattccagcc tccttcaggc agcgccttgg cgccgtagcg
      121 aaccatcgtg ccgccgtcga gcacgccgct cacgaacgga tgctgcttga agcggttgaa
      181 cgccatgtgc gggtcgaaca gcggatcgtg atagtccagc ccgacgacga agccgaccga
      241 gaggcgccct tccggcatgg catacatgaa cccgccgccg aactcgtcgt gcctcagcgg
      301 gtatcccatc gtgtggatca cggtgccagc ctcgaggcgc tttgccggga cttcccacag
      361 ttccttcaga ccgaccgcga actgcgccgg ctcacggccg cgatcgagtg cgagcgtgcc
      421 gatgacgtcc ttcgtcaggt tgccgcgcac gccctcacag aagatcgtga tcttcgcgag
      481 gatgtccacg ccccactcga acgtcggctt tctcgtcccg tcccgggccg tgccgcggtc
      541 acccgtcctg acgccgatca cgcgggtccc gtcgaacagc acctcccggc cgccgaatcc
      601 gctgaagacg tcgacgccct ccgcctcgac ccgttccgac aaccagcgca cgagcgcgtt
      661 caacgacacg atgacatggc cgtggttctg gagcggcggg ggcacgattg gaaaccggag
      721 ccgggacttc cccgtgagga agtagacctg gtcgcgacga acgggcgatg cgaacgcggc
      781 agctccgatg tcgaagtcgg gcacgagctg ccgcaacgcc gaggggtcga gcacggcgcc
      841 cgacaagctg tgggcgcccg catcgcgagc cttctcgatg acggcgatcg acagcggctc
      901 gctacgccct ttctgaagct gcgccaggcg cagggcggca gacagtcctg cgggaccgcc
      961 tccgacgatg aggatgtcga cttcgagagt ctctcgcatg aagccgtgaa cggcctactg
     1021 cttgtcgagt tcagtaatca gcgccggcac gatctcgaac aggtccccca cgataccgta
     1081 gtcggcgacc tcgaagatcg gagcatcggc gtccttgttg atcgcgacga tggtccgcga
     1141 gcctttcatg cccacgaggt gctggatcgc ccccgagata ccgagcgcga cgtagagctt
     1201 gggggctacg gtctggccgg agctgccgat ctgacgctcc atcggaagcc atccgctgtc
     1261 gcaaatcggc cgcgacgccg ccacttccgc tgacatggcc cgcgccagct tttcggcaac
     1321 cgcgatgttc tcctgcgact tgatcccccg cccgacggcg acgatgcgct cggcctggcc
     1381 gagatcgacg gcctgcctcg cttccttgaa gggaggttct ggtgtttggc ggatcgtcgc
     1441 ggcgtcgatc gagacctcca tgcgcgtcac gccacccggc cggtcacccc gctgcaccgc
     1501 atccgcccgg aaggcgccaa tctggaatgt caccaaccgc ggaccgttgc cctcgtgtcg
     1561 cacctcggcg gcaagcttgc cctgaaacac aggccgcacg aacgtggcag acgagccgct
     1621 gcccctgatg ccggtcacgt cagtgacgag cggcgctccc atccggactg ccgccatcgg
     1681 cgcgaaatcg cgggtctgat atgtgtgcgg cagaagcacg taggacggac tgactgcggc
     1741 gacaacctgc tggagagcct gggtgaagcc gtccggggta tagccggcaa gcgcggggtg
     1801 atcgactgcc aggacgtcca accccgccga cgccagttcc gacgccgcgt tgtcagtcgc
     1861 ctctccgaac acgacggcct tgacgggcat gccgccggcc agttgctgcg ccgcagccac
     1921 cgcttcccag ctggcgcggt tgagcgcgcc ggcccgctgc tcggcgacga ccagaatcac
     1981 agggctcgcg cctcgtcgcg gagaagacgg gccagctctc gcgcgatgtc ggcagcagac
     2041 ccggtgagca ttttcgtctg cttggacctg gccggaagcg acagccccag gatccgctgc
     2101 gacgccgcga gcccgggcgg gagcggcacc cgccggatct ctttcttctt ggcggccatg
     2161 atgcccttga gcgtcgcgta acggagttga ttgatgccgc tttgaatcgt cagcaccgca
     2221 gggagcggca tgccaatcca ctggaaccag ccgccctcga gctcccgctt gacgcgaagg
     2281 cgtgccgcct gccccgggcc ggacgagggg acgatctgca cctccatgat gatcgtcgag
     2341 tgcggcacgc cgagccgctc ggccagcatg acgcccacct gcgcgtgccc ctggtcgtcg
     2401 gactgcagcc cggtgagcac gaggtcgaag tgctcgtcgg ccatggccga cgccagggcg
     2461 gccgacgtga tcgaggcatc cgcgctcgcg agcgcctcgt catcaacgtg cagggcgcgg
     2521 tcagccccgc gcgccagggc ctcgcgtatc acctgctgca cccgcgcagg gcccgcggcg
     2581 caggcgacaa cttccccgct atgcttctcg cgcagcctca gcgcctcttc gagcgcgaac
     2641 gcatcgggct cgttcagctc gaagctggcg tcctgctcgc ggatccaggc cttgtcctcc
     2701 ctcagccgcg gctgccattc cctcgttggg acctgcttga tgcacacggc gatcttcata
     2761 tcgtgggcct ctcagatcca cgcggccacg gagtcactac ccgtcgaatt tcttgaacag
     2821 cagcgcggcg ttggtgccgc cgaaaccgaa ggaattcgac agcgcgtacc gaatcgacat
     2881 cggccggccg gtgtgaggga cgtagtccag gtcgcattcg ggatccggat cgtcgaggtt
     2941 gatggtgggc ggcgcgacct gatggtgcac cgcgagcgcc gagatgccgg cctccaggcc
     3001 gccggcgccg ccgagcaggt gccccgtcat cgacttggtc gacgacacgg ccagcttgta
     3061 ggcatggtcc ccgaacagct tcttgatcgc gagcgtctcg agacgatcgt tgtacggcgt
     3121 ggacgttccg tgcgcgttga tgtagtccac gtcctccggt ggaatcccgg ccttgcagac
     3181 cgccttcttc atgacccgca cgccgccgtc gccgtcctcg gagggcgccg tgatgtgata
     3241 cgcgtcgccc gacatcccgt agccgacgat ctcggcgtag atgtgcgccc cccgtttgcg
     3301 cgcaaagtcg agctcttcca ggatgagcat gccggcgccc tcgccgatga tgaacccgtc
     3361 gcgatccttg tcgaagggcc ggctcgcgcc gcgcgggttg tcattgcgcg tggacagcgc
     3421 gcgcagcgcg ccgaacccgc cgacgctcat cggcgtaatc gcagcctcgg agccgccggc
     3481 gatcatcgcc tcggcgtcgc cgcgcctgat gatctcgaac gattcgccga tggcgtgcgc
     3541 ggaggccgag caggccgtac acgtcgccag gtttggcccc ttggcgccga accggatcga
     3601 gacctggccc gccgcgaggt tgatgatggc cgagggaatg aagaacggcg agatcctccg
     3661 cgggccgcca ttcagcaggg cgctgtgctc acgctcgatg gtctggaagc ctccgatccc
     3721 ggagccgatg aagacgccga tatcgggggc attcgatggc gtgatctcca gccgcgagtc
     3781 gtccatcgcg aactgcgacg cggcgacggc gtactggatg aagacgtcca tcttcttgac
     3841 gtccttcttg tcgatgtagt cgagagcgtc gaatccccgt acttcggccg cgatctgcgt
     3901 tgcgaacccc gtgacgtcga acttcgagat ccgatcgacc ccgctcgtgc ccgccacgag
     3961 cgccttccag gtggcgtcgg tgccgatgcc gagcgacgac accaacccga tgccggtgat
     4021 gacgacccgc ctgctcaacg cccctccgct actttttctt gccgtgagcc tcgatgtagt
     4081 cgatggcttc cttgacccgg gtgatcttct c
//
LOCUS       DBNGSH010000699         3268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4319814, whole genome shotgun sequence.
ACCESSION   DBNGSH010000699 DBNGSH010000000
VERSION     DBNGSH010000699.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4319814"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="AC16CY_16080"
     CDS             <1..313
                     /locus_tag="AC16CY_16080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3355368.1"
                     /translation="AAGVKIAFGTDAGVSVGWVAHTELADMVAAGMTPAQVLTAATRT
                     TAEILRFNRLGTIAAGKSADFIVLDANPLDDITNTRRIADVYSRGDRIDRAALAAGWT
                     R"
     gene            353..2257
                     /locus_tag="AC16CY_16085"
     CDS             353..2257
                     /locus_tag="AC16CY_16085"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HIR3355369.1"
                     /translation="MTRTVWLLVLGVAAVVGVRAQQPSTPQVEWRHYGGDPGGMRYSP
                     LTGIDAPNLSRLQVAWQWKHWETALKEYGTTPGQFESTPLMIDGTLYVTTPYNSIAAL
                     DAETGQERWRFDGHAYELGQLLSGSGWKLRGTAFWRDGGELRIFLNSRHRLVQLDART
                     GKPVTTFGNGGAVSLTDGLPRISDVTHASQSSPPVVYRDLVIVGSQVPDRVQLPDPMG
                     YVQAFNARTGRRVWTFSVIPQSAKDPGAETWENESWRRNGHANVWAPMALDEPRGLIY
                     LPTSTPTSDYYGGGRPGANLFAESLVCLDAATGQMKWHFQTVHHGLWDWDLPTQPNLV
                     TLTVGGRRIDAVAQVTKRGDTFVFDRVTGAPVWPIEERPVPTDTDVPGEKPYPTQPFP
                     TRPRPFVDQGVTQEDANNLTPEIRRLAQDEMRKFRIGPIFTPPSLKGTLQRPSQGGGA
                     NWGGAAFDPETGYLIVRANNAVGVNRVAKNDGSDPLVGVDYSHVFAGRTSATLPGGLP
                     LVSPPYAVLAAIDLNRGEIVWKVPLGDNPAVRNHPLLKGVALPDRLGSSGNSGGAMVT
                     RSGLVFIGGGDGYFYAFETKTGREVWREKIPYASTANPMTYRTQSGKQFIVMATGTGE
                     NNALLAFTVK"
     gene            2339..2755
                     /locus_tag="AC16CY_16090"
     CDS             2339..2755
                     /locus_tag="AC16CY_16090"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3355370.1"
                     /translation="MRGFIVSCGLFVCAVLASAQAQRTIWDGVYSDEQAKRGEQLYAE
                     HCARCHGDGLGGVESAPALTGPAFYANWEGESLNALFERIRMSMPQDNPGSLSRVQNA
                     DIVSHMLRAGGYPAGKTPLEGQAGTLGQIKVLTYKP"
     gene            2914..>3268
                     /locus_tag="AC16CY_16095"
     CDS             2914..>3268
                     /locus_tag="AC16CY_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HIR3355371.1"
                     /translation="MALGLAAVVTTAWTMVPAQGQTGAPKGEWRTYGGDLGHTRYAAL
                     DQITAANFSKLEVAWRFKTDNLGPRPEFNFQSTPLMVGGRLFSTAGTRRAVVALDAAT
                     GELLWVHGEDEGARGA"
BASE COUNT          571 a         1048 c         1146 g          503 t
ORIGIN      
        1 cgcggccggc gtgaagatcg ccttcggcac ggatgcggga gtatcggtcg gctgggtggc
       61 gcacaccgag ctcgccgaca tggtggccgc ggggatgacg ccggctcagg tgctcacggc
      121 agccacgcgg accacggccg agatcctccg tttcaatcgc ctcgggacga tcgcggcggg
      181 gaagagcgcc gacttcatcg tgctcgacgc gaacccgctc gacgacatca cgaacacgcg
      241 gcggattgcg gacgtgtatt cgcgcggcga caggatcgac cgcgcggcgc tggccgccgg
      301 atggacccgg tgaagggatg tgaaggggtc tgaccccttg ctgggagtgc acatgactcg
      361 aacggtgtgg ttgctcgttc ttggcgtggc ggcagtcgtc ggcgtgcgag cacagcagcc
      421 atcgacaccg caggtcgagt ggcggcacta cggcggcgac cccggcggca tgcgctactc
      481 gccgctgacc ggcatcgatg caccgaacct ctcgcgcctt caggtcgcgt ggcaatggaa
      541 gcactgggag acggcgttga aagagtacgg gaccactccc ggccagttcg agagcacgcc
      601 gttgatgatc gacgggacgc tgtatgtcac gactccctac aacagcatcg ccgcgctcga
      661 cgccgagacg ggccaggagc gatggcgatt cgacggccac gcctacgagt tgggtcagct
      721 gctgtcgggc agcggatgga agctgcgcgg caccgcgttc tggcgcgacg gcggagagct
      781 tcggatcttc ctgaacagcc ggcaccggct cgtgcagctc gacgcccgga cggggaagcc
      841 ggtgacgacg ttcggcaacg gcggcgccgt gtcgctgacc gacggtctgc ctcggatctc
      901 cgacgtcacg cacgcgtcac aaagctcgcc gccggtcgtc tatcgagacc tcgtcatcgt
      961 cggcagtcag gttcccgatc gcgttcagct gcccgatccg atgggctacg tgcaggcgtt
     1021 caacgcgcgc acggggcggc gggtctggac cttctccgtg attcctcagt cggcgaaaga
     1081 cccgggcgcc gagacgtggg agaacgagtc gtggcggcgc aacggccacg cgaacgtgtg
     1141 ggcgccgatg gcgctcgacg agccgcgcgg gttgatttac ctgccgacgt ccacgccgac
     1201 gagcgactac tacgggggcg ggcggcccgg cgccaacctg ttcgccgagt ccctggtgtg
     1261 cctcgacgcg gcaacgggcc agatgaagtg gcatttccag accgtgcatc acggcctgtg
     1321 ggactgggat ctgccgacgc agcccaacct ggtcacgctg acggtcggcg gccggcgcat
     1381 cgacgccgtc gcgcaggtca ccaagcgagg cgacacgttc gtcttcgatc gcgtcaccgg
     1441 cgccccggtg tggccgatcg aggagcggcc ggttccgaca gacaccgacg tgccgggtga
     1501 aaagccgtat cccacgcagc cgttccccac acgaccgcgc ccgttcgtcg accagggcgt
     1561 gacgcaggag gatgcgaaca acctgacgcc cgagattcga aggctggcgc aggacgagat
     1621 gcgcaagttc cgcatcggtc ccatcttcac gcccccgtcg ctcaagggga cgctgcagcg
     1681 cccgagccag ggaggcggag ccaactgggg cggcgcggcc ttcgatcccg agaccggcta
     1741 cctgatcgtg cgggccaaca acgccgtcgg cgtcaatcgc gtcgcgaaga acgacggctc
     1801 cgatccgctg gtcggcgtcg actactcgca cgtcttcgcg gggcgaacgt cggccacgct
     1861 tcctggcggc ctcccgctcg tgtcgccgcc ctatgccgtg ctcgcggcga tcgacctgaa
     1921 ccggggcgag atcgtctgga aagtcccgct cggcgacaac ccggccgtcc ggaatcaccc
     1981 gctgctcaag ggggtcgcgc tcccggaccg gctcggctcg tcgggaaact ccggtggcgc
     2041 gatggtgacg cgaagcgggc tcgtcttcat cggcggaggc gacggctact tctatgcgtt
     2101 cgagacgaag acgggcaggg aggtgtggcg tgagaagatt ccctacgcca gcacggccaa
     2161 cccgatgacg taccgcacgc agtcgggcaa acagttcatc gtcatggcca ccggcactgg
     2221 cgagaacaac gcgctgctcg cttttaccgt gaagtagtgt acgtagagtc cggcttcagc
     2281 cggaccgtga agtgcgtagt gtccggcttt cggccggacc ggggggacca tcgtgaagat
     2341 gcgcggattc atcgtgtcat gcgggttgtt tgtctgtgcc gtgcttgcga gcgcgcaggc
     2401 gcagcggacg atatgggacg gcgtgtactc cgacgaacag gccaagcgcg gagagcagct
     2461 gtacgctgaa cactgtgccc ggtgccacgg cgacggcctg ggtggagtcg aatcggcgcc
     2521 ggcgctgacc ggccctgcct tctacgccaa ctgggaaggc gaatcgctga acgcgctctt
     2581 cgagcggatc cgcatgtcga tgccgcagga caatccgggc tctctgtcgc gcgtgcagaa
     2641 tgccgacatc gtgtcgcaca tgctgcgagc cggaggctac ccggccggga agactccgct
     2701 cgaggggcag gcgggaacgc tcggacagat caaggtactc acgtacaagc catagacaac
     2761 tggacggacg tgaaccacag aggcgcagag agcacagaga atccaatcat cagacctgaa
     2821 agaccagact ctgtgtcctc tgtgtctctg tggtgagcgt ccgtgatcag aagaaggaag
     2881 agcaaagcgg ggtcgcgtat gtggcgatta cgaatggcgt tgggattggc ggccgtcgtg
     2941 accaccgcgt ggacgatggt ccccgcgcaa ggacagaccg gcgcgccgaa aggggagtgg
     3001 cggacctacg gcggcgatct cggccacacg cgctatgccg cgctggatca gatcaccgcc
     3061 gcgaacttct cgaagctcga ggtggcgtgg cgcttcaaga ccgacaacct gggcccccgt
     3121 cccgagttca acttccagtc gacgccgctc atggtcggcg gacgcctgtt ctcgactgcc
     3181 ggcacgcggc gcgcggtcgt tgcgctcgac gccgcgaccg gcgagctgct gtgggtgcac
     3241 ggtgaagacg aaggggcgcg cggcgcgg
//
LOCUS       DBNGSH010000700         1813 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1773261, whole genome shotgun sequence.
ACCESSION   DBNGSH010000700 DBNGSH010000000
VERSION     DBNGSH010000700.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1773261"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..>1813)
                     /locus_tag="AC16CY_16100"
     CDS             complement(225..>1813)
                     /locus_tag="AC16CY_16100"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3355372.1"
                     /translation="LYVVRQVVILEPSPPGWISLRLLTWSAAMLSGVTTVLLWLHASA
                     LRNALDPRAMPALERTTTVFAVATLVFLSLGFAQVAARRRYRPTVAILFTIATTCSIV
                     VPLVLRGPGVPRLPRAGAVASAGLVADESRRVVLLCLDGASLDVISPAVAAGGLPNFG
                     RMIDHGASMHLATTLPTQPEPVWASVMTGMWPSAHGVRAAARYRPFGGDVALEVLPDY
                     LFTQALVRFGLLIEEPHTSAGLRAQPVWRIAAKHGVRTGLIGLPLTHPAADAAGFIVS
                     DRFYRQSERVVALDEEPAVAPPPLEDVARSVLAAGARTQGLRVTLDLLPGTGESGSTI
                     EADRLHHQLARELDDAERVQLLAVRYAGLDAVAHYYLRYARPEAFGDVSEEERRRFGR
                     VLDDYYGYLDSLVGEAIASLRDGDLLFVISGFGMEPLTLGKRVLERLVGDPRFSGTHE
                     RSPDGFLLAYGTPVVAARQGRGAVVDVAPTLLYFLGLPVARDMDGFPRTDLFTPAFNQ
                     QRTITFIPTYGIQEDVSGRR"
BASE COUNT          319 a          585 c          666 g          243 t
ORIGIN      
        1 atgcgatccg catccattac gactggcatg cgggcctcgc ggtgagtgtg gagcctgtat
       61 cgaggaagac gcgtgcgggc atgtgcacct ctttgcggac tctctgggcc tcgttaaaga
      121 agtgaaccta ttgtagcagg gggcaggggg cagggggcag ggggcagagg gcagagggca
      181 gagggggcag ggggcggggg gcagggggcg gcagattcag gggatcatct gcggccggaa
      241 acgtcctcct gaatcccgta ggtcggtatg aacgtgatcg tcctctgctg gttgaaggcc
      301 ggcgtgaaga gatcggtacg cgggaagccg tccatatccc gggccacggg caggccgagg
      361 aagtacagaa gcgtgggggc aacgtcaacg acggcgcctc gcccctgccg cgccgccacc
      421 acaggggtgc cgtaggccag caggaatccg tcggggctgc gttcgtgcgt gccgctgaat
      481 cgcgggtcgc cgaccagccg ttcgaggacg cgcttgccca acgtcagcgg ctccatcccg
      541 aacccggaga tcacgaacag caggtcaccg tctcgaagcg aggctatcgc ttcgccaacg
      601 agggaatcga ggtacccgta gtagtcgtcg agaacccggc caaagcgccg gcgctcctcc
      661 tctgacacgt cgccgaatgc ctcgggccgg gcgtacctca agtagtaatg ggcaaccgcg
      721 tcgagccccg cgtaccgcac ggcgagcagc tgcacgcgtt cggcgtcgtc gagctcgcgc
      781 gcgagctggt ggtgcagccg atcggcttcg atcgtggagc cgctttcgcc ggtccccggc
      841 agcaggtcca gggtgactcg aagtccctgg gtccgcgcgc cggcggcgag cacgctgcgg
      901 gccacgtcct cgagcggggg cggcgccacg gccggctcct cgtcgagggc cacgactcgc
      961 tcggactggc ggtagaaacg gtcgctgacg atgaagcctg cggcgtcggc ggcagggtgg
     1021 gtcagcggca aaccgatgag ccccgtccgc accccgtgct tggcggcgat gcgccaaacg
     1081 ggctgcgcgc ggaggcctgc ggaggtgtgc ggctcctcga tgagcaggcc gaaccgaacg
     1141 agcgcctgcg tgaacaggta gtcgggcagg acctcgagcg cgacatcgcc gccgaagggc
     1201 ctgtatcgtg ccgccgcgcg cacgccgtgg gcggacggcc acatgccggt catgaccgat
     1261 gcccacaccg gctcgggctg tgtcgggagc gtggtcgcca gatgcatcga cgcaccgtgg
     1321 tcgatcatcc ggccgaagtt cggcagcccg ccggcggcga ccgccggtga aatcacgtcg
     1381 agcgaggctc catcgaggca caacagcacg acgcggcggc tctcgtcggc aacaaggccc
     1441 gccgacgcga ccgcgcccgc gcgagggagc cgcggcaccc ctggcccgcg caataccagc
     1501 ggcacgacga tggaacaggt ggtggcgatc gtgaagagaa tcgccacagt cgggcgatac
     1561 cgtcgacggg cagcgacctg cgcgaagccg agcgacagga acacgagcgt tgcgacggca
     1621 aagaccgtgg tcgttcgttc gagcgccggc atggcccggg ggtcgagcgc gttccgcagc
     1681 gccgatgcgt gcagccacaa gaggaccgtt gtcactccgc tcaacatggc cgcgctccac
     1741 gtgagcagcc gcaggctgat ccacccggga ggcgacggct ccaggatgac gacctggcgt
     1801 accacgtaca acg
//
LOCUS       DBNGSH010000701         2794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_924667, whole genome shotgun sequence.
ACCESSION   DBNGSH010000701 DBNGSH010000000
VERSION     DBNGSH010000701.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_924667"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="AC16CY_16105"
     CDS             <1..1573
                     /locus_tag="AC16CY_16105"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3355373.1"
                     /translation="GLDFTHVNGASEEKFLPEIMGPGGLFVDFDDDGWLDIFLVDGGS
                     LAAAAVAKRARHRLFRNRGNGTFEDVTARSGIAHHAYGMGACAGDYDNDGLIDLYVTN
                     VGPNVLYRNTGGGRFTEVPNAAGAGTSIWSTSCAFFDMDRDGDLDLFVTSYVDDFTRG
                     RFCGVKGPPPIRDYCHPLLYSPLTNTLYRNTGRKTFEDISRQSGIAPHRGNGLGVAVS
                     DVDEDGWPDVFVANDAMPNFLFRNAANGTFTETAALAGVAVASDGRARAGMGPAFADF
                     AGSGRMGLIVTNHETEMHSLFANVDGRLFSDATVRSGVGPATRPYVGFGVAFLDYDND
                     TRLDIAIANGHVMANAAQVRAGGTFAQRNLLLRNTGERFEDMRQQAGPGFSIELVSRA
                     LAVGDIDNDGDLDVLITNNGAGPNLLLNEGGVGNAILVRVIGTKSNRSALGTRLTLTT
                     GQRRQIREVQSGSSYLAQNDLRAHFGLAGASRSERLDIRWPNGATETVKDLPANQIVT
                     IREGQGVVSRVPFSR"
     gene            1614..>2794
                     /locus_tag="AC16CY_16110"
     CDS             1614..>2794
                     /locus_tag="AC16CY_16110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3355374.1"
                     /translation="MFGLVLAVVASPVTAAAQRDAFFSALVPLYRTLDGVYGDEGPLI
                     TGRLGAMASALARWDEEIREAERQLRPKLTGADAQTALQIHTVLASLYMERGRYADAL
                     REFDEDLRLDPTRAPFHRFRGLILQAMSRPAEAADAFRTAWRLEPADPANAYRLIAYR
                     SSQTTPQELERALETLALVESELVGKTRPAAHTPFITISGIVDDAGAMAFAPAAYASG
                     VSLVVRGQFDAGLAALRAAAAADPLVVDPALRSQAAARGIESLRQGLAPAAIEQLESA
                     AAGARDSSEAYRVLATAYWLNGDIEKCLQHLRDAVRLNPRDERSWLVLARTLEETGRP
                     AEADEVLRRAVAAVPDAGALRWELAAAAGRRQRTEEADVAAIEMVDRYVLLVGRGELY
                     SA"
BASE COUNT          465 a          949 c          955 g          425 t
ORIGIN      
        1 cggcctcgac ttcacccacg tcaacggcgc gagcgaagag aagttcctgc ccgaaatcat
       61 ggggccgggc gggctgttcg tcgatttcga cgacgacggc tggcttgaca tctttctcgt
      121 ggacggcggc tccctggccg ctgccgccgt ggcgaagcgc gcacgccacc gcctcttcag
      181 aaaccgcggc aacggaacgt tcgaggacgt gaccgccagg tccggcatcg cgcatcacgc
      241 gtatggcatg ggcgcctgcg ccggcgacta cgacaacgac ggcctgatcg acctgtatgt
      301 caccaacgtc ggaccaaacg tgctgtatcg caataccggg ggcggacgct tcaccgaagt
      361 gccgaatgcc gccggcgccg gaacatcgat ctggagcacg agctgcgcgt tcttcgacat
      421 ggaccgcgac ggagacctgg atctgttcgt caccagctac gtggacgact tcactcgcgg
      481 acggttctgc ggggtgaagg gaccgccgcc gatcagggat tattgccacc cgctgctcta
      541 ctcgccgttg accaacacgc tctaccgcaa tacgggccgg aagacgttcg aagacatcag
      601 caggcagagc ggcatcgcgc ctcaccgcgg gaacgggctc ggcgtggccg tgtccgacgt
      661 agacgaggat ggctggccgg acgtcttcgt cgccaacgac gccatgccga acttcctctt
      721 tcgcaacgcg gccaacggga cgttcacgga aaccgccgcc ctggccggcg tggccgtggc
      781 gtcggacggc agggcgagag ccggcatggg acccgcgttc gccgacttcg ccggcagcgg
      841 gcggatgggg ctgatcgtca ccaaccatga aaccgagatg cacagcctct tcgcgaacgt
      901 ggacggccgc ctgttctcgg acgccaccgt gcgcagcggc gtggggccgg ccacgcgtcc
      961 ctacgtcggc tttggcgtcg cgtttctcga ctacgacaac gacacgcggc tcgacatcgc
     1021 gattgccaac ggccacgtca tggccaacgc cgcgcaagtc cgcgcgggcg gcaccttcgc
     1081 acagcggaat ctcctgctgc gcaacaccgg cgagcgcttc gaggacatga ggcagcaggc
     1141 cggtccgggc ttctcgatcg agctcgtgag ccgcgcgctg gccgtgggcg acatcgacaa
     1201 cgacggcgac ctcgatgtgc tgatcaccaa caacggcgcc ggtcccaacc tgctgctgaa
     1261 cgagggcggc gttggcaacg cgatcctggt ccgcgtcatt ggcacgaaaa gcaaccgcag
     1321 cgcgctcggc acgcggctca ccctcacgac cggccagcgg cggcagatcc gcgaggtgca
     1381 atcagggtcg agctacctgg cgcaaaacga cttgcgtgcg cacttcgggc tcgcaggcgc
     1441 cagtcgatcc gagcgtctcg acatacgctg gccgaacgga gcaaccgaga cggtcaagga
     1501 tcttcccgcc aatcaaatcg tgacaatccg cgagggccaa ggtgtcgtca gccgagtgcc
     1561 gttcagtcgc tgacaggcgc ccggcgtgcg ccactgtgcc tcggtgtgtc gtagtgtttg
     1621 ggcttgtact ggcggtcgtc gcgtccccgg taaccgccgc cgcgcagcgc gacgcgttct
     1681 tttccgcgct cgtgccgctc taccggacgc tcgacggcgt gtatggcgac gagggacctc
     1741 tcatcacggg ccgtctggga gcgatggcgt ccgctctcgc gcgatgggac gaggagatcc
     1801 gcgaggcgga gcgccaactg cggccgaagc tgacgggcgc cgatgcgcag acggcgctgc
     1861 agatccacac ggtgctggcc tcgctgtaca tggagcgcgg acgttacgcg gacgccctcc
     1921 gtgaattcga cgaggacctg cggctcgatc cgacgcgcgc gcccttccac cgcttcaggg
     1981 gcctcatcct gcaggcgatg tcgcgccccg ccgaggcggc cgatgccttc cgtacggcgt
     2041 ggcggctcga gccggctgat ccagcgaatg cgtatcggct gatcgcgtac aggtccagcc
     2101 agacgacgcc acaggagctc gagcgcgcgc tcgagacgct ggcgttggtc gagagcgagc
     2161 tggtcgggaa gacacgcccg gccgcacaca cacccttcat caccatcagc gggatcgtcg
     2221 acgacgcagg cgcgatggca ttcgcgcctg ctgcgtatgc cagcggcgtg tcgctcgtcg
     2281 tgcgcggcca gttcgacgcc ggcctcgccg cgctgcgggc ggcagccgcc gccgatccgc
     2341 tcgtggtcga tccggccttg cgatcgcagg cagcggccag gggcatcgag tcgttgcggc
     2401 agggtctcgc cccggcggcc atcgagcagc tcgagtcggc cgcagcgggc gcgcgcgatt
     2461 cgtcggaagc gtatcgcgtc ctggcgacgg cgtattggct gaatggagac atcgagaaat
     2521 gcctgcaaca cctccgagac gccgtgcgcc tgaatcctcg cgacgaacgc tcctggctgg
     2581 tgctcgcccg gacgctcgaa gaaaccggcc ggcccgctga agcggacgag gtgcttcgcc
     2641 gcgccgtggc cgcggtgccg gacgcgggag cgctgcgatg ggagcttgcg gccgccgccg
     2701 gcaggcgtca gcgcacagag gaggccgacg tcgccgcgat cgaaatggtc gatcggtacg
     2761 tattgctcgt cggcaggggc gagctgtaca gcgc
//
LOCUS       DBNGSH010000702         6648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3301811, whole genome shotgun sequence.
ACCESSION   DBNGSH010000702 DBNGSH010000000
VERSION     DBNGSH010000702.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3301811"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="AC16CY_16115"
     CDS             <1..933
                     /locus_tag="AC16CY_16115"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3355375.1"
                     /translation="LLAPLAALGPLMGRRGGFRPAVLGLLPPRLRALMSVAPRVSVRA
                     LLGGRPASADAIGGERLKVAVLEGCVQRLAFAEVNDATVRVLAAEGCRVVAVPAQGCC
                     GALPLHSGSIDQARELARHNIEVFEAAGVDRIVVNAAGCGSAMKEYGELFADDRAWAD
                     RARAFSAKVRDVSQLLVELGEPRAARRPIPARVVYHDACHLAHGQGVRAEPRALLQAI
                     PGVELLTPAEADICCGSAGIYNLVQPEAAAELGARKARHIAALSPDMIATGNPGCMLQ
                     IAAAARGLGHDWPIVHPIELLDASIRGVEPRGSG"
     gene            complement(960..2528)
                     /locus_tag="AC16CY_16120"
     CDS             complement(960..2528)
                     /locus_tag="AC16CY_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3355376.1"
                     /translation="MQVYIVNPSHVSFGIGVITPRWLYVLAAATPQKYGEPQLIDETL
                     EPFDTAAIAANDVVGIGIHTGNALRGYEIGVAARAAGAHVVFGGIHATLYPEEARDLG
                     GAHAVVTGDGELAWPAVLRDCASGAPRDRYDGGRVEGETFLPGRWDLLPPGRYMWASV
                     QTVRGCPKHCSFCSVWRTDGQRPRQRHVDRVIEEIVDLRRRGFRFIALADDNFYPVTL
                     DDLRMAARRHDPGALQRLQAIRAERFALMEGLARLPDDTVFFTQITMEAAEDPEFLEA
                     MRQAHIKGALVGVEAVTPEGLKDIYKDFNESGESLVERLRAFRRHGIHVLGSFIFGLP
                     SDRPETFEATLSVAERADLTFAQFVMLTPFPGTLDFAAWEKSLGEEPPRVGGIPVSRH
                     WLIPQARRPKVYMNHPVMDADEIRRRTQAVWDRFYSLPSIWRRSRGAVSSIKARLAFL
                     LISKIYRQMYANTGIATDSARVHRSARWARLMAIPCRRLFAGRPMPELRVPSQASPDT
                     TGRMLVMVGGDPTH"
     gene            complement(2561..2770)
                     /locus_tag="AC16CY_16125"
     CDS             complement(2561..2770)
                     /locus_tag="AC16CY_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355377.1"
                     /translation="MVTRPVQMNAYEFVVVSALRAQQLLAGCTPRLEGDHSAATMAQM
                     EVAAGCVGRAGDEGATPSPQCRWQL"
     gene            3065..4765
                     /locus_tag="AC16CY_16130"
     CDS             3065..4765
                     /locus_tag="AC16CY_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR3355378.1"
                     /translation="MWQHNYEPVAGSLGVSALFAAIPILVLFFMLGVARKPAWMAASS
                     ALLSALLVALTVYGMPAQLAVISAIYGAAFGIFPIAWIVFASIMLYRLAVDTGKFEVI
                     KDSIGGLTNDRRLQAMFIAFSFGAFIEGAAGFGAPVAISGAMLAGLGFNPFYAAGICL
                     LANTAPVAFGSIGIPVTTLANVTGLPVMPLSAMVGTLCAMVSIIIPGYLIIVMAGVKR
                     AVEVLPAIIACGVSFAGVQFYVSRTRGPELTDILSSLTCIVVMVVVLKLWSPKNIMRL
                     EGDTPAATTAKRHGPGAVMTAWVPYLMLVVFVLVWGETAIKHRINQWANGFVPSSWTT
                     VPGTENPPVATRLAVPGLHNLIERVPPVVGKAGPYAALYELNWLSASGTACFLATLAT
                     AVVLRVRPRKVVKTYVDTFKQLKLAMLTIACMLGLAYLMNYSGMTSTLGLAFATTGVA
                     FPFFSAVVGWLGVFLTGSDTSANALFGNLQVVTANALGLNPVLTASVNSAAGVMGKMI
                     SIQSIAVAVAATGMTSADESRLFRFTIKHSVALMIVMGIISMLFAYVFTGSVPVVPPK
                     "
     gene            4762..6618
                     /locus_tag="AC16CY_16135"
     CDS             4762..6618
                     /locus_tag="AC16CY_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355379.1"
                     /translation="MSRHALGVLFVHGIGEQPRGDTLLTFSEPLIRCVRGWMEGRDIG
                     SADVVESKLSASQLGSEEPAHTVLEIAVRDRSPEKWLLAESWWADEFSRPPFMKLAAW
                     LLTIGPWAIISHASRWLGDAPRGQVPYQLLKVVLAVPLSMLMQIVVALLAVVAWLPIP
                     VVRRAVSGFLLRVTGTLGDSYVLLEDPVQKAAAIGAVRHNVRWLADRCDDVAVVAHSQ
                     GAAIARLALAERRHPKVCVLVTFGSGVAKLTELGSMSTETRARSVRTILWVSLVAIAL
                     LPRALSLTTDDDARSILWTLYLFLPLAMMVAALTVVWGNIKAWPERAAELSLSPLDWI
                     DYWATADPVPNGPLAPADKVRGLRQFKITNRINWLTDHSTYWENWDEFVMPVARELDQ
                     RAGSGVFTVETPAKIPGIPGRRVRVALLALARAAIVFLATPIMLYAFRGQLEVFGRDV
                     ILQSLSERPLTKPVAELLTGLGSFVGLAIGPLVELDAVTLDRWGHASVAALLLVAMMA
                     AWYRFCVLPLWENWNGQCFEWLCRPARIPAHFADRALRPFLLSVVAAVPVLLAARALF
                     QPNARLLLEEVLNVVVASPVVVMAFFMVVFVGALVFQVLAVAVRGVRRLVGR"
BASE COUNT         1001 a         2192 c         2246 g         1209 t
ORIGIN      
        1 ctgctcgcgc cgctcgctgc gctcggcccg ctcatggggc gcaggggggg attccgcccc
       61 gcagtgctcg ggctccttcc gccccggctt cgggcgctca tgtcggtggc accccgagtc
      121 agcgtgaggg cgctgctcgg aggccggccc gccagcgccg acgccattgg tggcgagcgc
      181 ctcaaggtgg ccgtgctcga aggctgcgtc cagcgtctcg cgttcgccga agtcaacgac
      241 gccacggtcc gcgtgctggc cgccgaaggg tgccgcgtcg tggccgtgcc cgcgcagggc
      301 tgctgcggcg cactaccgct tcattccggt tctatcgatc aggcacgcga gctggcgcgg
      361 cacaacatcg aggtgttcga ggccgccggc gtcgatcgca tcgtcgtgaa cgccgccggc
      421 tgcgggtccg cgatgaagga gtacggggag ctgttcgccg acgatcgtgc ctgggccgat
      481 cgcgcgcgag cgttcagtgc caaggtacgg gacgtctcgc agctgctcgt ggagctcggc
      541 gagccgcgcg ccgcccgccg ccccattccc gcccgtgtgg tgtaccacga cgcgtgccat
      601 ctggcgcacg gacagggcgt ccgcgccgag ccgcgcgcgc tgctccaggc cataccgggc
      661 gtagagctgc tgaccccggc ggaggccgac atttgctgtg gaagcgccgg catctacaac
      721 ctggtccaac cggaagcggc cgcggagctg ggggcccgca aggcgcgtca catcgcggcg
      781 ctgtcgccgg acatgattgc caccggcaac ccgggctgca tgctgcagat tgcggctgcg
      841 gcacgggggc tcgggcacga ctggccgatc gttcacccca tcgagcttct tgacgcgtcg
      901 atcagagggg tcgagccgcg cgggtccggc taaagccgga cactacgtac tcaagggcgc
      961 tagtgcgtcg gatcgccgcc gaccatcacg agcatgcgcc cggtcgtgtc gggtgacgcc
     1021 tgtgacggaa ccctcaactc gggcatgggt cgccccgcga acagccggcg gcagggaatc
     1081 gccatgagac gcgcccaccg cgcggaccgg tgcacgcgcg cactgtcggt cgcgatgccc
     1141 gtgttggcat acatctgccg gtagatcttc gaaatgagca gaaaggcgag cctcgccttg
     1201 atcgaactga ccgcacctcg cgatcgcctc cagatgctcg gcagcgagta gaaccgatcc
     1261 cacactgcct gcgtccgtcg acggatctcg tccgcgtcca tgacggggtg gttcatgtac
     1321 accttcggac ggcgtgcctg gggaataagc cagtgacgcg acacaggaat gccgcccacg
     1381 cgcggcggct cttcgccaag tgacttctcc cacgcggcaa aatccagcgt gccggggaac
     1441 ggcgtcagca tgacgaactg ggcaaacgtc aggtcggccc gttccgcgac cgacagcgtc
     1501 gcctcgaacg tttccgggcg atcgctgggc agtccgaaaa tgaacgatcc gagcacgtga
     1561 atgccgtgcc gtcgaaaggc acgcaggcgc tcgacgagcg attcgccgct ctcgttgaag
     1621 tccttgtaga tgtctttcag gccttctggc gtgacggctt cgacgccgac cagcgcccct
     1681 ttgatgtgcg cctgacgcat ggcctcgagg aactccgggt cttcggcggc ctccatcgtg
     1741 atctgggtga agaacaccgt gtcgtccggc agccgtgcca acccttccat cagggcgaag
     1801 cgctcggcgc ggatcgcctg aagccgctgc agcgcacccg gatcgtgccg ccgcgccgcc
     1861 atccgcagat cgtcgagcgt gaccggatag aaattgtcgt cggcgagcgc gatgaaccgg
     1921 aatccacggc gtcgcaggtc cacgatttcc tcgatcaccc ggtcgacgtg gcgctggcgt
     1981 ggccgttgtc cgtcggttcg ccagacggag cagaacgaac agtgcttcgg acatccgcgg
     2041 acggtctgga ccgacgccca catgtaacgt cccggaggca gcaggtccca gcgcccggga
     2101 agaaatgtct caccctcgac gcgaccgccg tcgtaccggt cgcgcggcgc ccccgacgca
     2161 cagtcgcgaa gcacggccgg ccacgccagt tcgccgtccc cggtgacgac cgcgtgcgcc
     2221 ccgccgagat cgcgggcttc ctctggatac agagtggcgt gaatgccgcc aaacaccacg
     2281 tgcgcacctg ccgcacgcgc ggcgaccccg atttcgtacc cgcgcagcgc attgcccgtg
     2341 tggatgccga tcccgacgac gtcgttggcc gcgatcgcag ccgtgtcgaa cggctcgagg
     2401 gtctcgtcga tcagctgcgg ttcgccatat ttctgcggag tggcggcggc caggacatac
     2461 agccaccggg gggtgatcac gccgatgccg aaggagacgt ggctgggatt gacgatgtag
     2521 acctgcatcg tctagtggtc tgtccttcgc ggatggtcca ctagagctgc cagcgacact
     2581 gcggtgacgg cgtcgctccc tcgtcaccgg ctcgccccac gcagcccgcg gcgacttcca
     2641 tctgcgccat cgtcgcggca ctgtggtcgc cctcgagacg aggggtgcag ccggcgagca
     2701 actgctgcgc gcggagcgca gagaccacga cgaactcgta cgcgttcatt tgcaccggac
     2761 gggttaccat cgcgtcgtct cgttgcgcat ggactgcacg cgcgtgctgg aagcgagaag
     2821 acgtcgcagg cttggaacgg cggagtctgg tggcttcatg ggttcctcga ggccatggtg
     2881 aggaacccgc gggactgcgg gaggctcaag acagctgcgg ataccttaac acgggacagc
     2941 ccggcacgcg cagctacttc gcggcgctct ccggatatga ttgccaccgg caaccggggc
     3001 tgcgtgccgc agatccccga ccaccaacca cgaaacacca aacacccggc cggatagagg
     3061 agctatgtgg cagcacaact atgaacctgt cgccgggagc ctcggcgtgt cggcgttgtt
     3121 tgccgcgatc ccgatcctgg tgctgttctt catgctcggg gtcgcgcgca agcccgcgtg
     3181 gatggcggcg tcgtcggcgc tgctctccgc ccttcttgtc gcgctgaccg tttacggcat
     3241 gcccgcgcag cttgccgtca tctcggcgat atacggcgcg gcgttcggta ttttcccgat
     3301 cgcatggatc gtcttcgcgt ccatcatgct ctatcgcctg gccgttgaca caggcaagtt
     3361 cgaggtcatc aaggactcga tcggagggct gaccaacgac agacgcctgc aggcgatgtt
     3421 catcgccttc tccttcggcg ccttcatcga gggcgccgca ggctttggcg ccccggtcgc
     3481 catctcgggc gcgatgctcg ccgggctcgg cttcaacccg ttctatgcgg cagggatctg
     3541 tctgctggcc aacaccgcgc ccgtggcgtt tggatcgatc gggatccccg tcaccacgct
     3601 ggcgaatgtg acggggctcc cggtgatgcc gctgagcgcg atggtgggga cgctctgcgc
     3661 gatggtctcg atcatcattc ccggctacct gattatcgtg atggccggcg tcaagcgcgc
     3721 cgtcgaggtg ctgccggcga tcatcgcgtg cggcgtgtcc ttcgccggag tgcagttcta
     3781 cgtgtcacga acgcgcggac ccgagctgac cgacatcctg agctcgctca cgtgcatcgt
     3841 cgtcatggtg gtcgtgctca agctgtggtc gccgaagaac atcatgcgcc tcgaaggcga
     3901 caccccggcc gcaacgacgg cgaaacggca cggcccgggt gccgtcatga cggcgtgggt
     3961 cccgtacctg atgcttgtgg ttttcgtgct cgtgtggggc gagaccgcga tcaagcacag
     4021 gatcaaccag tgggcgaacg ggttcgttcc cagctcgtgg acgacggttc ccgggaccga
     4081 gaacccgccg gtggccacgc gcctggcagt gccggggctg cacaacctga tcgagcgcgt
     4141 cccgcccgtc gtcgggaagg ccgggcccta tgcggccctc tacgagctga actggctgag
     4201 cgcatcgggc acggcgtgct tcctcgccac gctcgcgacg gcggtcgtgc tccgcgtgcg
     4261 cccgcggaag gtcgtgaaga cctacgtcga tacgttcaag cagctcaagc tcgcgatgct
     4321 gaccattgcc tgcatgctgg ggctggcgta cctgatgaac tactcaggca tgacctcgac
     4381 gctcggcctc gcgttcgcga caaccggcgt ggcgttcccg ttcttcagcg ccgttgtcgg
     4441 gtggctcggc gtgttcctga ccgggagcga cacctccgcc aacgcgctct tcgggaacct
     4501 ccaggtcgtg accgccaacg cgctgggcct gaacccggtg ctcacggcgt cggtgaactc
     4561 ggcagcaggc gtgatgggga agatgatctc gattcagagc atcgccgttg cggtggccgc
     4621 caccgggatg acgtcggctg acgagagccg gctcttccgc ttcaccatca agcacagcgt
     4681 cgccttgatg atcgtcatgg ggatcatctc gatgctcttt gcgtacgtgt tcacaggcag
     4741 cgtccccgtc gtgccaccaa agtgagtcgc cacgccctcg gcgtgctctt cgtgcacggc
     4801 atcggcgagc agcctcgcgg cgataccctg ctgaccttca gcgagccgct gattcggtgt
     4861 gtccgtgggt ggatggaggg ccgcgacatc ggcagcgcgg acgtcgtcga gtcgaagctg
     4921 agcgcgtcgc agttggggtc cgaagaaccg gcacacacgg ttctcgagat cgccgtgcga
     4981 gaccgatccc ccgagaaatg gttgctcgcc gagagctggt gggcggacga gttcagccgt
     5041 ccgccgttca tgaagctggc cgcgtggctc cttacgatcg gtccctgggc catcatttcc
     5101 cacgcaagcc gatggctcgg cgatgcgccc cggggacaag tgccgtacca gctgctgaag
     5161 gtcgttctcg ccgttccgtt gtcgatgctg atgcaaatcg tcgtggcctt gctcgcggtg
     5221 gtggcgtggc tgccgattcc ggtggttcga cgcgccgtct cggggttcct cctcagggtc
     5281 accggaacgc tgggcgacag ctacgtcctg ctcgaggacc ccgtccagaa ggcggcggcg
     5341 atcggcgcgg tccggcacaa cgtgcggtgg ctggctgacc gctgcgacga cgtggccgtg
     5401 gttgcgcact cccagggcgc tgcgattgcg aggcttgcgc tggccgagcg acgtcacccc
     5461 aaggtgtgtg tgctggtgac gtttggatcg ggagtcgcca agctcaccga gctcggatcg
     5521 atgtccacgg agacgcgggc acggagcgtg cgaacgatac tctgggtgtc gctggtcgcg
     5581 atcgcgctgc tcccgcgcgc gctgtccttg acgaccgacg acgatgcccg gtccattctg
     5641 tggacgctgt acctgttctt gccgctggcg atgatggttg cggccttgac cgtggtgtgg
     5701 ggcaacatca aggcctggcc cgaacgtgcc gccgagctgt cgctctcgcc gctcgactgg
     5761 atcgactact gggcgaccgc tgatcctgtg cccaacggtc cgctcgctcc tgccgacaaa
     5821 gtccgaggtc ttcgacagtt caagatcacg aatcgaatca attggctcac ggaccactcg
     5881 acgtattggg agaattggga cgagttcgtg atgccggtcg cgcgcgagct ggatcaacgc
     5941 gccggcagcg gcgttttcac ggtggagacg ccagcgaaaa tcccagggat tccgggacgc
     6001 cgcgttcgag tcgccctgct cgccttggcc cgcgccgcga tcgtctttct ggcgacgccg
     6061 atcatgctct acgcgtttcg cggccagttg gaggtgttcg gacgcgacgt catcctgcaa
     6121 tcgctctcgg aacgcccctt gaccaagccc gtcgccgagc tgctgaccgg gctgggatcg
     6181 tttgtcggcc tcgcgatcgg accgctggtg gagctcgatg cggtgacgct ggatcgctgg
     6241 ggtcacgcgt ccgttgctgc actcctcctg gtcgccatga tggccgcctg gtatcgcttc
     6301 tgcgtgctgc cgctgtggga gaactggaac gggcagtgct tcgagtggct gtgtcgaccg
     6361 gcgaggattc cggcccattt cgcggaccgg gcgctgcggc cgttcctgct gtcagtggtt
     6421 gcggcggttc cggtgctgct cgccgcgagg gcgctgttcc agccgaatgc gcggctgttg
     6481 ctcgaggagg tcctcaacgt cgtggtcgcc tcgcccgtcg tggtgatggc tttcttcatg
     6541 gtggtgttcg tcggggcgct ggtgttccag gttctggcgg ttgccgtccg cggggttcgg
     6601 cggctcgtcg gcaggtagcc gcagtccgga aaagtgttcg cgccgttt
//
LOCUS       DBNGSH010000703        10029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2283135, whole genome shotgun sequence.
ACCESSION   DBNGSH010000703 DBNGSH010000000
VERSION     DBNGSH010000703.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2283135"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="AC16CY_16140"
     CDS             <1..922
                     /locus_tag="AC16CY_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3355380.1"
                     /translation="GFDPLARTLNWIDRQGRVEMIPAPPHAYAQPRLSHDGTRVVASN
                     VRDPENNLLVLDLTRGIVTRVTTDAALDVTPTWTPDDSWIIFSSNRGGGGQSLWRQAS
                     NGTGNPERLTQAAFVQGAPVVTPDGRHVLFTEQTEDGGADTMQLTLATKQVTPVLHDR
                     SREMLAEVSPDGRWLAYTSNRSGPVEVYVKPYPITDGAPWQVSTAGGDHPAWARDGGE
                     LFLFYMTPDNALMSVSVQTASPTWRAGQPVRVLEPEQLPRSSTTRNYDPAPDGRRFLV
                     LGRPIPAPDPPGLVVVQHWDEELKQRVPTK"
     gene            complement(962..1195)
                     /locus_tag="AC16CY_16145"
     CDS             complement(962..1195)
                     /locus_tag="AC16CY_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355381.1"
                     /translation="MNRNVWTVAGVFVFALAALNVILGKYLVPSMAVATEKGTVAGWW
                     LIWVVIAISVMSAVSLGCFLIAAGLGKVPETRH"
     gene            1315..2100
                     /locus_tag="AC16CY_16150"
     CDS             1315..2100
                     /locus_tag="AC16CY_16150"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3355382.1"
                     /translation="MRIRHLAWCFAAAAVMALAPAASTQTPSPGAAADPLRVFASNGV
                     KSVMESLVPEHEKRAGRAVAVTFGTSASIRQAIAAGEPFDVAVLTTEVLEELRKAEHI
                     AAGSSVVIGRSGIGVGVRSGAARPGIATPEAMKRTLLGAKSVTYAADGASRVHIERML
                     ETLGITEAMKPKSVLEQGSVRATARVARGEVEIVLTLVSEILPVSGIELVGPLPAAFQ
                     SYVSFSAGVGARTRDASAAKALIDALVSSRVDEVFSTRGIERR"
     gene            complement(2102..2572)
                     /locus_tag="AC16CY_16155"
     CDS             complement(2102..2572)
                     /locus_tag="AC16CY_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355383.1"
                     /translation="MSAPAPRALLAPLALLAPLALIGCTQAQAEPPPFKAASSVIQLM
                     EGPIAHAAEDYWGSVSTIVDAKGITENFPRTDEEWEHVWAAAITIAESGNLLMMSPRA
                     KDQGDWMKWSAALVDVGMEAAKSAESKDPEQVLAVGERIYNVCTSCHMQYIKEE"
     gene            2689..4362
                     /locus_tag="AC16CY_16160"
     CDS             2689..4362
                     /locus_tag="AC16CY_16160"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HIR3355384.1"
                     /translation="MKRLGMLAALGAMLAAQANVSALIPEQVKIESGLVAGVTTANPN
                     VRVFKGVPFAAPPLGESRWKPPQPAAKWDGVFKADAFGPSCTAGGGGGRGRGAAPGRG
                     GAPGAPGAGAQAPAPQAAAQPAAPPRQPPASEDCLTVNIWTSASRAGDRRPVMVWIYG
                     GGFTGGSGGMQWYDGEDLAAKGPVIVTFNYRLGALGFFAHPELARESGRNASGNYGMM
                     DAIGALQWVKRNISAFGGDPENVTIFGESAGAIMIGALVGSPQAKGLFHRAIAQSGAW
                     MGLQMGRMRRGDDALAAGAKAMEAAGIKTIAELRAKPLAELPALGGGGLVIDGYIIPE
                     DLSYTFANGRQNDVDVLAGSNKDENTFGGGGGRGRGGAAAAPGAAVDAYVAQAKTRYG
                     GDLAGTYLKLYPASADADVAAATAQAYNDEVNWNMRQFAAAQMRRGKKGYAYFFTRVP
                     LRNGQPQPNGATHTAEISYVFNHPYSNGTLEWNDVDRKLADVMSSYWVNFAAKGDPNG
                     TGLPSWSAYKDHSSGRVMVLGDTVQAEAAAPTAKLTFFDAAHARMMKSN"
     gene            complement(4439..5584)
                     /locus_tag="AC16CY_16165"
     CDS             complement(4439..5584)
                     /locus_tag="AC16CY_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355385.1"
                     /translation="MYRRVLASAGIATTTALIALTSLPVSGQSAGVKAPALVVTANNG
                     GPGTPYTTPKTPWGDPDLQGVWSSDDATMPMARPQNVEGRLYLNDGEFAARQKQIQQG
                     VTNAEQNAVGSFRGDFARRAFRQTSLIVDPPDGRTPAFTEEAQKRRAPRDQGTFGDGP
                     FNSTEDFTLYDRCITRGIVGSVMRVIYGNGNRIVQAPGMVAISYEMIHDTRVFYTDGR
                     PHIGQAIRQYLGDSRARWEGDELVVETTNLTDKTSIGPNGNGLRHSDKMKITERFRRV
                     ADQILQYQITVDDPVTYAAPFTLSLPLTPLDGGVLLPYECHEGNYAVRQSLGAERAED
                     ARVEADLRRGVIRPRRPVQDGLGVGGQPIGEAGPGGLVPGRGAGPGI"
     gene            complement(5674..6816)
                     /locus_tag="AC16CY_16170"
     CDS             complement(5674..6816)
                     /locus_tag="AC16CY_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355386.1"
                     /translation="MTRRVLASIGIAIVIALAASLPVAGQSASAPPLVITAYNGGPPI
                     PYTTPRTPWGDPDLQGVWSSDDTSGIPMARAQGGGLYQSDEQWAARQKQTQQGIQNAL
                     NAIGTFRGDYARRAFRQTSVIIDPPDGRLPAVTPAAEARRAPRDRGTFGEGPFNTPED
                     FTMYDRCITRGIVGSVLRVVYGNGNRIVQAPGMVAISYEMIHDTRVFYTDGRPHISPS
                     IRQLLGDSRARWEGDELVVVTTNLTDKTSIGLNGNGLRHSDKMKITERFKRVADDVIQ
                     YQMTVDDPVTYVSPFTMSLPLTPLEGNILLPYDCHEGNLAVLQSLGGERAEDKALADD
                     LSRGIIRPRRGVQESGAGGGGGGGRGRGAGAGGRAGGAPPEGDVDR"
     gene            6966..7223
                     /locus_tag="AC16CY_16175"
     CDS             6966..7223
                     /locus_tag="AC16CY_16175"
                     /inference="COORDINATES: protein motif:HMM:NF021474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HIR3355387.1"
                     /translation="MKRTNVVLDEQLLEEAVRVSGERTYSRTIERALEELVRRAKARG
                     IDQLAGSGLWKGDLSEMRRDLPVAVRESRAVYRPRKRRAAD"
     gene            7210..7623
                     /gene="vapC"
                     /locus_tag="AC16CY_16180"
     CDS             7210..7623
                     /gene="vapC"
                     /locus_tag="AC16CY_16180"
                     /inference="COORDINATES: protein motif:HMM:NF047959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3355388.1"
                     /translation="MLLIDTSAWIELFRRGSRITLDDLADDRDRIVTCLPVIQEVLQG
                     FDDERAFQIARTAMSALPCVDSPLSRAVFDSAVDIYRLARRVGVTVRSSVDCLVAASA
                     SRHRLTVVHRNRDYTQLAKVVAFDHVDISPLLTRT"
     gene            7628..8446
                     /locus_tag="AC16CY_16185"
     CDS             7628..8446
                     /locus_tag="AC16CY_16185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3355389.1"
                     /translation="MRHLATILAVVGCGLQAGVRAQQPSERPSFEVASIRPSAPGTRY
                     GLRILPGGRLSVTHATVQRLILTADVLHESQLAGGPSWLYADGFDIEARSSAGASTDR
                     PEMMGRLRTLLNERFGLRTQTELRDAAVYALVLARADKKLGEFIRPSAIDCEAADAAT
                     KLALSRGEVPAGPRCGVSFSGGAEVTVMRYAAMTMSQIAMGLSQTAGRMVVDQTGLSG
                     IFDVELRYTSDPLSSSDAPSIFTATQEQLGLRLESRRMSVPVMVVDRVERPTEN"
     gene            8606..>10029
                     /locus_tag="AC16CY_16190"
     CDS             8606..>10029
                     /locus_tag="AC16CY_16190"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3355390.1"
                     /translation="MRRLGSALLVVASLGVAVLVAQSGGSGGSRGTSRPFTTWRAYGG
                     GAHSSQYSALNQINESNVSQLDVAWTYPVNGNIIFNPIVVDGVMYLQGTGNAVVALDA
                     ATGKEIWQHANQGGIGARGFNYWESPDRKDRRILYLNAGHLTAINAENGQTITSFGDN
                     GRVDLRVALYRQAANPLQTSNPGRIFENLMIVSLPAQGAQYDATPADVQAYDVRTGKI
                     AWVFHSIPHAGEFGYDTWPPGHYKVGGGGHNWSEFTVDEENGIAFIPFGTGRFDFYGG
                     DRKGDNLFANSLVALDARTGRRLWHFQTIHHDLWDYDLPQAPKLLTIRQNGRNVDIVA
                     QASKQGFLYVFERRTGRPIWPIEERPVPKSDVPGEWASPTQPFPTKPAPFARQSFTEK
                     DINPYLPAAEREALVQRFRTLRNDGLFTPPSFEGSIQLPGHNGGANFGGSAVDPARGE
                     MYVVSKSLPTVLRIALPDQGGRGGG"
BASE COUNT         1657 a         3251 c         3445 g         1676 t
ORIGIN      
        1 cggcttcgat cctctggcgc gcaccttgaa ctggatcgac cggcagggcc gggtggagat
       61 gattccggcg ccgccgcacg cctatgcgca gccgcgactg tcccacgacg gtacacgcgt
      121 ggtcgcctcg aacgtgcggg atcccgaaaa caacctcttg gtcttggatc tgacgcgggg
      181 gatcgtgacg cgcgtcacca ccgacgccgc gctggacgtc acacccacgt ggacacccga
      241 cgacagttgg atcatatttt cctccaatcg cgggggtgga ggacagagcc tgtggcgtca
      301 ggcctcgaac ggcactggga accccgagcg cctcacgcag gcagctttcg tccaaggtgc
      361 tccagttgtc acgcccgacg gacggcacgt gttgttcacc gagcagaccg aagacggtgg
      421 cgccgacacc atgcagctga cactggccac caaacaggtc acgccggttc tgcatgaccg
      481 atccagggag atgcttgcgg aggtctcgcc ggacggacgc tggctggcct acacctccaa
      541 cagatccgga ccggtggagg tttacgtcaa gccgtatccg atcaccgacg gcgccccgtg
      601 gcaggtttcc acggcgggtg gcgaccaccc ggcctgggcg cgtgacggcg gagagctgtt
      661 cctgttctac atgacgccgg acaacgctct gatgagcgtg tcggtgcaga ctgccagccc
      721 cacatggcgt gccgggcagc cagtgagagt ccttgaaccg gaacaattgc ctcgctccag
      781 caccaccagg aactacgatc cggcgccgga cggtcggcga ttcctggtgc tcgggcggcc
      841 aatccccgct cccgacccgc caggtcttgt cgtggtgcag cactgggacg aggagttgaa
      901 acagcgcgtg ccgacgaaat agcgcggtgc tgaccgggga gccggccgtt cccgggcgcg
      961 cctagtgcct ggtctcggga actttcccaa gtccggccgc gatcaggaag cagccgagcg
     1021 acacggctga catcaccgag atcgcaatca ccacccagat cagccaccag ccggcgaccg
     1081 tacccttctc cgtggcgacg gccatcgagg gaaccagata cttgcccaga atcacgttga
     1141 gcgccgcaag ggcgaacacg aagactccag cgacggtcca cacgtttcgg ttcattcgat
     1201 ggattctgcc acagtcgggg tcagatcttg attcttgcgc gtgctcagag caggaccaca
     1261 tgacttcagc cgcgcaaaaa tcaagatctg accccatgcg ctaggatgtt tcccatgcgc
     1321 atccgacacc ttgcgtggtg cttcgccgct gcagccgtca tggcgctcgc accggccgcg
     1381 tctacccaga caccttcacc aggtgccgcc gccgatccac tgcgcgtgtt cgcgtcgaac
     1441 ggcgtgaagt cggtgatgga gtcgctcgtt ccggaacatg agaagcgtgc cggccgggcg
     1501 gttgccgtga cattcgggac ttcagcgtcg atccgccagg cgatcgcggc cggcgagccg
     1561 ttcgacgttg ccgtgctgac gactgaggtg ctggaggagc tgagaaaagc ggaacacatc
     1621 gccgcagggt cgagtgtcgt gattggacgc tcgggcatcg gcgtgggtgt acggtcgggc
     1681 gcggcacggc ccggcatcgc gacgccggag gcgatgaagc gaacgctgct cggcgcgaag
     1741 tcggtgacct atgccgccga cggcgccagc cgcgtccaca tcgagcgcat gctcgagacg
     1801 ctcggcatca ccgaggccat gaagccgaag tccgtgctcg agcagggatc ggttcgcgcg
     1861 acggcgcgcg tcgccagggg cgaggtggag atcgtgctga cgctcgtgag cgagatcctg
     1921 ccggtgtccg gcatcgagct cgtcgggccg ctcccggcgg cgtttcagag ctacgtgtcg
     1981 ttctcggccg gtgtgggtgc caggacgcgg gacgcgagcg cagcgaaggc gctcattgac
     2041 gccttggtca gctcacgcgt cgatgaggtt ttttcgacca gggggatcga gcgccgatga
     2101 actactcctc cttgatgtac tgcatgtggc agctcgtgca gacgttgtag atccgctcgc
     2161 ccaccgcaag cacctgctcc gggtccttcg actcggccga cttggcagcc tccatgccga
     2221 cgtcgacgag cgcggccgac cacttcatcc agtcgccctg atccttggct cgcggcgaca
     2281 tcatcagcag gttcccggac tcggcgatcg tgatggcggc agcccagacg tgctcccatt
     2341 cctcgtcggt gcgagggaag ttctcggtga tccccttcgc gtcgacgatc gtcgagacgg
     2401 agccccagta gtcttcggcc gcgtgcgcga tcggtccctc catcaactgg atcacgctgg
     2461 aggccgcctt gaacggcggc ggctccgctt gtgcctgcgt gcatccgatc agggcgagtg
     2521 gggcgagtag ggcaagtggg gcgagcaggg cgcgtggggc aggtgccgac atgcagttgt
     2581 catccttcct gtgagcagaa cgtcatgaac aggcgtattc tacgcgagac agaaaccggg
     2641 ctcgcaaggg ccgacgctgg attgccatct ttctctagag ggactgcgat gaaacgacta
     2701 ggaatgctcg cggcactcgg cgcgatgctt gccgcccagg cgaacgtctc ggcgctgatt
     2761 ccggagcagg tgaagattga gagcgggctg gtggccgggg tgacgacggc gaacccgaat
     2821 gtacgggtct tcaaaggcgt cccgttcgcg gcgccgcctc tcggcgaaag ccgctggaag
     2881 ccgccgcagc cggcggcgaa atgggatggc gtgttcaagg ccgatgcctt cggaccctcg
     2941 tgcacggccg gtggcggcgg cggacgcgga cgcggcgcag cgcccggacg cggcggcgca
     3001 cccggcgcgc cgggtgcagg agcacaggct ccggcgccac aggctgccgc ccagccggcg
     3061 gcgcctccga gacagccccc tgcgagcgag gactgcctga ccgttaacat ctggacgagc
     3121 gccagccgcg caggcgaccg ccggcccgtg atggtgtgga tctacggtgg cggcttcacc
     3181 ggaggatcgg gcgggatgca gtggtacgac ggcgaggatc tcgccgcgaa gggtccggtg
     3241 atcgtcacct tcaactatcg tctgggagcg ctcggcttct tcgcgcaccc cgagctggca
     3301 agagagtcgg gccgcaacgc ctcaggcaac tacgggatga tggacgccat cggcgcgctg
     3361 cagtgggtga agcggaacat ttcggccttc ggcggcgatc ccgagaacgt gacgatcttc
     3421 ggcgaatcgg ccggcgcgat catgattggc gcgctcgtcg gatcgccgca agccaagggg
     3481 ttgttccatc gcgcgatcgc ccaaagcggc gcgtggatgg ggctgcagat ggggcggatg
     3541 aggcgcggcg acgatgcgtt ggccgcgggc gcgaaggcga tggaagcggc cggcatcaag
     3601 acgattgccg agctccgcgc caagcccctg gccgagttgc ccgcgctcgg cggcggggga
     3661 ctcgtcatcg acggctatat catcccggaa gatctgtcct acacgttcgc caacggcagg
     3721 cagaacgacg ttgacgtgct cgccggctcc aacaaggacg agaacacgtt cggcggcggt
     3781 ggcggccgcg gccggggcgg ggctgccgcc gcgccaggcg cagccgtgga cgcgtacgtc
     3841 gcgcaggcga aaacccgcta cggcggcgac ctcgccggca cctatctgaa gttgtatccc
     3901 gccagcgccg acgccgacgt cgccgccgcg accgcgcagg cctacaacga cgaggtcaac
     3961 tggaacatgc gccagttcgc cgcggcccag atgaggaggg gcaagaaggg gtacgcctac
     4021 ttcttcacgc gcgtcccgct ccgcaacggt cagccccagc caaacggcgc gacgcacaca
     4081 gcggagatct cgtacgtgtt caaccatccg tactcgaacg gtacgctgga atggaacgac
     4141 gtcgatcgca aactggcgga cgtgatgtcg tcctactggg tgaactttgc ggccaaggga
     4201 gatcccaacg gcacggggct gccgtcgtgg tcggcgtaca aggaccattc gagcggccgc
     4261 gtgatggtgc ttggcgacac cgtgcaggcc gaagccgccg cgcccacggc gaagctcacg
     4321 ttcttcgacg cggcgcacgc gaggatgatg aagtccaact gagaagactc ccaacttcca
     4381 acacacaact cccaaattca gacgaaggct tgggcgttgg aagttggggg ttgggagttc
     4441 agatcccggg ccccgcgcca cggcccggga ccaggccacc agggcccgct tctccgatgg
     4501 gctggccccc gacgccgagc ccgtcctgca cgggacgacg cggccggata acgccccttc
     4561 gcaggtccgc ttccaccctg gcgtcctcgg cgcgctcggc gccgagcgac tgccggaccg
     4621 cgtaattccc ttcgtggcat tcgtagggca gcagcacgcc gccgtcgagc ggcgtcaggg
     4681 gcagcgagag cgtgaacggc gccgcgtagg tcaccgggtc gtccacggtg atctggtact
     4741 gaagaatctg atccgccacc cgcctgaagc gctcggtgat cttcatcttg tcgctgtggc
     4801 gcagcccgtt gccgttcggg ccgatgctcg tcttgtccgt gaggttggtg gtctcgacga
     4861 cgagctcgtc cccctcccac cgtgcgcgcg agtcgcccag gtactgtcgg atcgcctgcc
     4921 cgatgtgcgg ccgcccgtcc gtgtagaaca cgcgcgtgtc gtggatcatc tcgtagctga
     4981 tggcgaccat gccgggggcc tggacgatgc ggttcccgtt gccgtagatc acccgcatca
     5041 ccgaaccgac gatgccgcgc gtgatgcagc ggtcgtacag ggtgaagtct tcggttgaat
     5101 tgaacgggcc atccccgaat gtgccctggt cccgcggcgc ccggcgcttc tgcgcctcct
     5161 cggtgaacgc gggcgtgcgg ccgtccggag gatcgacgat gagagaggtt tggcggaacg
     5221 cgcgccttgc gaagtcgccg cggaacgagc cgacggcgtt ctgctcggcg tttgtcacgc
     5281 cctgctggat ctgcttctgc cgcgcggcga actccccgtc gttcaggtaa agccggcctt
     5341 cgacgttctg cggccgcgcc atcggcatcg tggcgtcgtc gctgctccag acgccctgca
     5401 gatcgggatc tccccacggt gtcttcggcg tcgtgtacgg ggtccccggc cctccgttgt
     5461 tcgccgtgac gaccagtgcc ggcgccttca cgcccgcact ctgccctgat accggcagcg
     5521 acgtcagcgc gatgagcgca gtcgtggtcg cgatccccgc agacgcgaga actcgcctgt
     5581 acatgccatg tcctttcaat gtgctctccg cgcaggctgc agcctgcgca ctacgccgca
     5641 gatctactga agcctgcgcg ctacgccgcg gatctaccga tcgacgtcgc cttccggcgg
     5701 cgcgccgccg gcccggccgc cggctcctgc tccgcggcct cgtcctccgc cgcccccgcc
     5761 gcccgcaccg ctttcctgga cgccgcgccg aggccggatg atgccgcgcg acaggtcgtc
     5821 ggcaagggcc ttgtcctcgg cccgctcgcc gccgagcgac tgcagcaccg cgaggttgcc
     5881 ctcgtggcaa tcgtagggga gcaggatgtt gccttcgagc ggcgtcagcg gcagcgacat
     5941 cgtgaagggg ctgacgtagg tcaccgggtc gtccaccgtc atctgatact gaatgacatc
     6001 gtcggccacg cgcttgaatc gttcggtgat cttcatcttg tcgctgtggc gcagcccgtt
     6061 gccgttgagg ccgatgctcg tcttgtcggt caggttggtc gtgacgacga cgagctcgtc
     6121 gccttcccac cgcgcgcgcg agtcgcccag gagctggcgg atgctgggag agatgtgcgg
     6181 ccggccgtcg gtgtagaaca cgcgcgtatc gtgaatcatc tcgtagctga tcgcgaccat
     6241 ccccggcgcc tggacgatgc ggttgccgtt gccgtagacg acgcgaagca ccgaaccgac
     6301 gatgccgcgc gtgatgcagc ggtcgtacat cgtgaagtct tcgggcgtgt tgaacggacc
     6361 ctcgccgaac gtgccgcggt cgcgcggagc acggcgggcc tcggccgccg gcgtgaccgc
     6421 cggcagccgg ccgtcaggcg gatcgatgat gacggaggtc tggcggaacg cgcggcgcgc
     6481 gtagtcgccg cggaacgtgc cgatggcgtt cagcgcgttc tggatgccct gctgcgtctg
     6541 cttctggcgc gcggcccact gctcgtcgga ttgatacagg ccgccgccct gcgctcgcgc
     6601 catcgggatg ccgctcgtgt cgtcgctcga ccagacgccc tgcagatcgg gatcgcccca
     6661 cggcgtcctc ggcgtggtgt acgggatcgg cggcccgccg ttgtacgcgg tgatgacgag
     6721 cgggggagcg gacgcgctct ggcccgcgac cggcagcgaa gccgccagcg caatcactat
     6781 cgcgatgccg atggatgcga gtacccgcct ggtcatgtcg agctccttaa actgaagagg
     6841 gcgtgcgtat tatacgggcg ggtagggcgg gtagggcgag tggggcgagt ggggacggaa
     6901 aggcaggatg cggcggcggc gaggcagcct tgcatctcga ccggcgccgc tggtagcctg
     6961 tgtgtatgaa acgtacgaat gttgtgcttg atgagcagct gctggaggag gcggtgcggg
     7021 taagcgggga gcggacgtac tcccgaacga tcgagcgggc gctggaagaa ctggtgcgcc
     7081 gggccaaggc ccgcggcatc gaccaactgg cgggctcggg cctgtggaag ggcgacctgt
     7141 cggagatgcg tcgcgacctc cccgtggccg tccgggagag ccgcgctgtc taccggccac
     7201 ggaagcgtcg tgctgctgat tgacacgagc gcctggatcg agctgttccg tcgcggcagt
     7261 cgcatcaccc tcgacgacct ggccgacgac cgcgaccgca tcgtcacctg cctgccggtg
     7321 attcaggaag tcctgcaggg tttcgacgat gagcgggcgt tccagattgc gcgaaccgcg
     7381 atgtcggcgc tgccgtgcgt cgactcgccc ctctcgcgtg ccgtcttcga cagcgcggtg
     7441 gacatctacc gcctggcaag gcgtgtgggt gtcactgtgc gttcgagcgt cgattgcctg
     7501 gttgcggcgt ccgcgtcgcg acatcggctc accgtcgtgc accggaatcg cgactacact
     7561 cagttggcca aagtcgtggc gttcgatcac gtggacatct cgccgctgct gacccgcacg
     7621 tgagtcgatg cggcatctgg caacgatcct cgctgtcgtc ggctgcggac tccaggccgg
     7681 agtgcgcgct caacaaccgt cggagaggcc gtcgttcgaa gttgcgtcaa tacgaccgag
     7741 cgcgccggga acgcggtacg gccttcgaat cttgcccggc gggcggctgt cggtgacaca
     7801 tgccaccgtg caacgcctga tcctcacggc tgacgttctt cacgaatcgc agctcgcggg
     7861 cgggccttcg tggctgtacg cggacggctt cgatatcgaa gcgagatcgt ccgccggggc
     7921 ttcgacggac cggccggaga tgatgggaag gctccgaacg ttgttgaacg agcgctttgg
     7981 attgcggacc caaacggagc ttcgtgacgc ggccgtgtat gccctggtcc tcgcccgggc
     8041 cgacaagaag ctgggcgagt tcattcgccc gtcggcgatt gactgcgagg cggccgacgc
     8101 ggcaacgaag ctggcgttgt cgcgaggtga agtgccagcc ggtccacgct gtggggtcag
     8161 cttctccggg ggagccgagg tgaccgtgat gcgatatgcc gcaatgacga tgtcgcagat
     8221 tgcgatgggg ttgtcgcaga cagccggtcg catggtggtg gaccaaactg gcttgtccgg
     8281 gatcttcgac gtggagctgc ggtacacctc ggatccgctc tcgtcgtcgg atgcgccgtc
     8341 gatctttacc gcgacgcagg agcaactcgg cctgaggctg gagagtcggc ggatgagcgt
     8401 tcccgtgatg gtggtcgatc gggttgagcg gccgaccgag aactgatgtc aatcgcgtcc
     8461 cgctcgaacc ttccgaacag gacctgctgg acccattgca cccactgaac cccctgaacc
     8521 cactgtgaga aagcagttac ggtagaattt tcgccagctc gagcgctcgg cagacgacag
     8581 atttcgctcg aatgaggacg gattgatgcg cagacttggc agcgcgctgc tggtggtcgc
     8641 ctcccttggc gttgccgtgc tggtggcgca atccggcggc agcggcgggt cgcgcggaac
     8701 gagccggcca ttcacgacgt ggcgggcgta cggcggcggg gcgcactcgt cgcagtactc
     8761 ggcgctcaat cagatcaacg agtcgaacgt ctcgcagctc gacgtggcgt ggacgtatcc
     8821 cgtgaacggc aacatcatct tcaatcccat cgtcgtggat ggcgtgatgt acctgcaggg
     8881 gacgggcaac gcggtcgtgg ccctcgacgc cgcgacgggc aaggagatct ggcagcatgc
     8941 caaccagggg gggatcggcg cgcgcgggtt caattactgg gagagccccg accgcaagga
     9001 ccggcggatc ctgtacctga acgcgggtca tctcacggcg atcaacgccg agaacggcca
     9061 gacgatcacg tcgtttggcg acaacggcag ggttgacctg cgcgtcgcgc tgtaccggca
     9121 ggcggcaaac ccgctgcaga cgagcaaccc cgggcggatc ttcgagaacc tgatgatcgt
     9181 ctcgctgccg gcgcaagggg cgcagtacga cgcgacgcca gccgacgttc aggcgtacga
     9241 cgtgcgcacg gggaagatcg cgtgggtctt tcacagcatt ccgcacgccg gcgagttcgg
     9301 gtacgacacg tggcccccgg ggcactacaa ggttggcggc ggcggccaca actggagcga
     9361 gttcaccgtc gacgaggaga acggcatcgc gttcatcccc ttcgggacag ggcgtttcga
     9421 tttctacggc ggcgatcgga agggcgacaa cctgtttgcg aattcgctgg tcgcactcga
     9481 tgcgcggacg ggccgccggc tctggcactt ccagacgatt catcacgacc tctgggatta
     9541 cgacctgccg caggcgccga agcttttgac catccggcag aacggccgca acgtcgacat
     9601 cgtggcgcag gcatccaagc agggattcct ctacgtcttc gagcgcagga ccggccgccc
     9661 gatctggccc atcgaggagc gccccgtgcc gaagtccgat gtgccgggag agtgggcgtc
     9721 gcccacgcag ccgttcccca ccaaaccggc gccgttcgcc cggcagtcgt tcaccgagaa
     9781 ggacatcaac ccgtatctcc ccgccgccga gcgcgaggcg ctcgtgcagc gattcaggac
     9841 gctcaggaac gacggcctgt tcacgccgcc aagctttgag ggttcgattc agcttcccgg
     9901 gcacaatggc ggcgccaact tcggcggctc cgccgtcgat ccggcccgcg gcgagatgta
     9961 cgtcgtgtcg aagagcctgc cgacggtgct gcgcatcgcg ctgcccgacc agggcgggcg
    10021 cggcggcgg
//
LOCUS       DBNGSH010000704         9138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3810647, whole genome shotgun sequence.
ACCESSION   DBNGSH010000704 DBNGSH010000000
VERSION     DBNGSH010000704.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3810647"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="tyrS"
                     /locus_tag="AC16CY_16195"
     CDS             complement(<1..815)
                     /gene="tyrS"
                     /locus_tag="AC16CY_16195"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR3355391.1"
                     /translation="MDLYRELTWRGLVSDGTEGVEEVLTKEKVSGYIGFDPTASSLHA
                     GNLLVMLALARLQRCGHSPIALVGGGTGLIGDPGGKTAERQLLSREQVESNVAGIRAQ
                     LARVLDFETPTNPARLVNNADWLTGLKAIDFMRDVGKHFTVNALLAKESIKRRVEGEG
                     GISYTEFSYVLLQAYDFLELYDRFGCTLQMGGSDQWGNITAGMDLIRRTRGGRTHGLV
                     MPLMTTASGTKFGKTEGGAIWLDAERTSPYEFYQFWLNTDDRDAIPYLKYFTFL"
     gene            871..1692
                     /locus_tag="AC16CY_16200"
     CDS             871..1692
                     /locus_tag="AC16CY_16200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3355392.1"
                     /translation="MPITRRTAIKAVLATTLGAAAGGVSYGVAVERHRLGITNATLPV
                     SGLPPSLDGLRIGFITDIHHSRLVPAADVIRALELTMSTQPDLVVLGGDYVTFGDRAF
                     VGPVAELLGSLRAPHGVFAILGNHDDDRDMAAALTGRGFVVLRDQRTDVSIRGERLSF
                     AGIRFWTRRTATIAAVLKGAPGTAFLLAHDPRRLVQAAELRVPAVLSGHTHGGQVVLP
                     GIGALARRRFPVLAGLAQRDETSIFVSRGVGTVYVPVRLNCPPEVALVTLRRALA"
     gene            complement(1667..2299)
                     /gene="purN"
                     /locus_tag="AC16CY_16205"
     CDS             complement(1667..2299)
                     /gene="purN"
                     /locus_tag="AC16CY_16205"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR3355393.1"
                     /translation="MTPDPINRRLGVLISGRGSNLQALIDAVGDGRLDASIVVVISNR
                     AEAEGLDRARRAGIEALHISHRGWPSRDAYDRALVDELRKRGVGLVCLAGFMRLLGPA
                     MIDAFPDAIVNIHPALLPAFPGVDAQRQAFEYGVKITGVTVHVVTRELDAGPILMQRP
                     VPVLETDSVETLSARILAEEHRVYPEAVKLVLDGGWTVEGRRVRPARAGA"
     gene            2400..3029
                     /locus_tag="AC16CY_16210"
     CDS             2400..3029
                     /locus_tag="AC16CY_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3355394.1"
                     /translation="MIFHVVNRSAKRALLFESPGDYAAFEHVLAAAIARSSVALFAYC
                     VMPNHWHLLLSPHVDGALSRCMHWLTTTHARRWQTVRGLDGQGAVYQGRFKSIPVGCD
                     EHFLWVCRYVERNALRASLVSRAEDWSWSSLARRHQDPLTSWLSEWPVPRPRDWSDIV
                     NRPQTDAELQAFKTAVAKNEPFGRDEWKRSTMARMGVLPARRGRPLVEK"
     gene            complement(3042..4049)
                     /gene="purM"
                     /locus_tag="AC16CY_16215"
     CDS             complement(3042..4049)
                     /gene="purM"
                     /locus_tag="AC16CY_16215"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3355395.1"
                     /translation="MDYKTAGVDIDAGNETVDRIRALARSTFTSAVLSDIGSFGGLFR
                     LDPGLARDPVLVSSADGVGTKLRVAFMAGRHGTVGVDLVNHCVNDILVQGARPLFFLD
                     YFGTGRLSPAVAESVVSGMARACRENGCALLGGETAEMPGFYADGEYDIAGFIVGVVE
                     RERLITGRTIAPGDVLIGLPSSGLHTNGYSLARRIVFDHLKLSIDDVVPELGGTIGDV
                     LLEPHRSYLPEIRPLLAGGRIKGMAHITGGGITDNLPRILPDGTAAVVRPSSWAVPPV
                     FTWLQEKGGVPHPDMLRTFNMGVGLILVSAPDHADALADVLAAPVIGEIVAGEPSVTY
                     L"
     gene            complement(4061..5248)
                     /locus_tag="AC16CY_16220"
     CDS             complement(4061..5248)
                     /locus_tag="AC16CY_16220"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3355396.1"
                     /translation="MKGTLSSADVLAAALAVTLVLLVGAAAPPYARGQAGATAADLPK
                     NIANLGDPDFPVRMRAAQLIRRTPASAAVPALAEAARKHTNEFVRYKSLVVLTSFNDP
                     GTRELMREMIRDPSDRVREIVYRWFEVRPDPRTATQLLASLDTEQAEFVRPALIGALG
                     ALGSTTAVQRPMIVETQRGLDFFRSAVIESLGRHRAAYALDAILNVARLMGPLQDDAV
                     LAIGRVGGSDAAETLKELTELTPEAALMKLAARCLVGESCGEQIAALVSAASAPKARP
                     SEIRTAIDALAAVAQAQNQPALAALMNLAAKQSSLRDRVASAVAGVALRQPDWTVSRL
                     DAADQATREAAILMLKDGFDSLEEDFAEEQFFAAVRAGYWRAAEGSSARTLAETLIQR
                     LEF"
     gene            complement(5223..6695)
                     /gene="purF"
                     /locus_tag="AC16CY_16225"
     CDS             complement(5223..6695)
                     /gene="purF"
                     /locus_tag="AC16CY_16225"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR3355397.1"
                     /translation="MFDKFRDECGVFGIFGHPEASNLAYLGLYALQHRGQESAGIAAS
                     DGSVIRVSRAMGYVHDAFDTAALAKLPGRMAVGHVRYSTAGESRIANAQPILVDSAHG
                     QLAVGHNGNIVNATELRDELVRNGAIFQATTDTEVIVHLFARSREESAETAIIDALSQ
                     VRGAYSFVMMTKDRLIGARDPHGFRPLALGRLGDAWVICSETCAMDLIGATYVRDIEP
                     GEIVVVTAHGLKSLKPFPPAPRAQCIFEHVYFARPDSYVFGESVNEIRTEFGRRLARE
                     SSVEADVVVPIPDSGVCAAIGYSEASGLPLRMGLIRNHYVGRTFIEPQQSIRHFGVRV
                     KLNPVRSILEGKRVVLVDDSIVRGTTSRKIVRMVRSAGAREVHMRISCPPTISPCFYG
                     VDTPRRSELIAATHTIEEIRKYLDADSVSYLSLQGLTGAVNGGSQSYCTSCYTGVYPV
                     AFPRDEEAYLQLALKLNPEPEEPEPLAFSIPDVERDPVVG"
     gene            complement(6796..9042)
                     /gene="purL"
                     /locus_tag="AC16CY_16230"
     CDS             complement(6796..9042)
                     /gene="purL"
                     /locus_tag="AC16CY_16230"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR3355398.1"
                     /translation="MTPDPITDSVLQRHGVTRDEYARIVSMLGREPNLTELGIFSVMW
                     SEHCSYKSSRVHLKTLPTTGRRVLQGPGENAGAVDIGDGLAAVFKIESHNHPSFIEPY
                     QGAATGVGGIIRDIFTMGARPIALLDSLRFGSLDDPLVRRTMAGVVSGIGGYGNAIGI
                     PTIGGEIGFDESYTGNPLVNVFCLGIARADEIIKGTASGTGNAVYYVGAKTGRDGIHG
                     ATMASAEFDEKSAEKRPAVQVGDPFMEKLLLEACLEVMKSGALVGIQDMGAAGLTCST
                     CEMGSRGGAGVEIDVSLVPQREAGMTPYEIMLSESQERMLLVVKKGREPEVEQIFGKW
                     DLHAVRIGEVTTGGRLQVKNRGQVVADIPNKALTDEAPMYRRPMATPSYIADVQRLDL
                     AKLGTPPAPGGVFKRLLASPGIASKQWVYRQYDHMVRTNTLVLPGMGAGVVRVKGTSR
                     ALALSADCNGRFVYLDPFAGAQLAVAEAARNVACAGGTPIGATNCLNFGNPEKPEIMW
                     QFARAVEGMGAACRALDIPITGGNVSLYNETDGKGVLPTPVIGMVGLIEDADRVVARA
                     FRDEGDVVVLLGDSRDELGGSEFLHVVHGQNRGLPPALDLSREAALQRVLVEGAAGGV
                     IRSAHDCSEGGLAVTLAECCFDSSLGVEVDVPGVSAAASYVDTATLFGESASRAVVSV
                     SPSKAAVLLAAAAAAGVPAARIGRVGGERIRISVDGRRVVDEPLMEAERLWSGSIASY
                     FELRKAIA"
BASE COUNT         1559 a         3077 c         2940 g         1562 t
ORIGIN      
        1 agaaaggtga agtacttgag atacggaatc gcgtccctgt cgtcggtgtt gagccagaac
       61 tgatagaact cgtacggact ggttcgttcc gcgtccagcc agatcgcgcc gccctctgtc
      121 ttgccgaact tggtccccga cgccgtcgtc atgagcggca tcacgaggcc gtgtgtcctg
      181 ccgcctcgcg tgcgacggat cagatccatc ccggcagtga tattgcccca ctggtcgctg
      241 ccgcccatct gcaaagtgca gccaaaccga tcgtagagct ccaggaagtc gtacgcctgc
      301 agcagaacgt agctgaactc ggtgtacgag attccgccct ccccttcgac gcggcgtttg
      361 atcgattcct tggccagcag cgcattgacg gtgaaatgct tgccgacgtc ccgcatgaag
      421 tcgatggcct tgagcccggt gagccagtcg gcgttgttga ccagtcgagc agggttggtc
      481 ggcgtttcga agtcgagcac acgcgccagc tgcgccctga tgccggcgac gttcgactcc
      541 acctgctcgc gcgacaacag ctgtcgctcg gccgttttgc cgcccgggtc gccgatgagt
      601 ccggtgccgc cgccgacgag cgcgatcggc gagtggccgc accgttgtaa ccgcgccagg
      661 gccagcatca cgagcaggtt tcccgcgtga aggctcgagg ccgtcggatc gaacccgatg
      721 tacccgctga ccttctcctt cgtgaggact tcttccacgc cttcagtgcc gtccgacacg
      781 agaccgcgcc acgtcagctc gcgatagagg tccatcggct attaactata ctgtctacag
      841 tctcgggtcc acggtctgcg gtcgtcgatc atgcccatca cacgccgcac agcgatcaag
      901 gcggtgctcg cgacgacgct tggcgccgca gccggtggcg tctcctacgg cgttgcggtc
      961 gagcgccatc gcctcgggat cacgaacgcg accctgccgg tgtcgggctt gcctccctcg
     1021 ctcgacggcc ttcggatcgg cttcatcacc gacatccacc atagccgact cgtcccggcg
     1081 gccgatgtca ttcgagcgct cgaactgacg atgagcaccc agcccgatct cgtggtcctc
     1141 ggtggcgact acgtcacgtt cggggatcgg gcattcgtgg gtccggttgc cgagttgctc
     1201 ggctcgctgc gcgccccgca cggcgtcttc gccatcctcg gcaaccacga cgacgacaga
     1261 gacatggcgg cggcgttaac gggcagggga tttgtcgttc tgagggatca gcggactgac
     1321 gtgtcgatcc gcggggagag gctgtcgttc gccgggatac gattctggac gcgccggacc
     1381 gcgaccatcg ccgccgtgct caagggagcg ccgggtacgg ccttcctgct ggcgcacgat
     1441 ccccgccgcc tcgttcaagc ggccgaactt cgcgtcccgg ccgtcctctc gggtcacacg
     1501 cacggcggtc aagtggtgct ccctggcatc ggcgcactcg cgcgccgccg cttcccggtg
     1561 ctggcgggcc tcgctcaacg ggacgagacg tccatcttcg tgagccgtgg agtgggaacc
     1621 gtgtacgtcc cggttcgact caactgccct ccggaggtcg cgctcgtcac gctccggcgc
     1681 gcgctggcct gacccgccgg ccttcgacgg tccacccgcc atcgagcacg agcttcacgg
     1741 cttccggata cacacgatgc tcctcggcca gaatccgggc tgaaagcgtc tcgacgctgt
     1801 cggtctcgag gaccggcacg ggccgctgca tcaggatcgg tccggcatcg agctcgcgag
     1861 tgacgacatg caccgtcacg ccggtgatct tcactccgta ctcgaacgcc tgccgctgcg
     1921 cgtccactcc agggaaggca ggaagcagcg ccggatggat gttgacgatc gcgtccggga
     1981 acgcgtcgat catcgccgga ccgagcaggc gcatgaaccc ggcaaggcag acgagaccca
     2041 caccccgttt ccgcagctcg tctacgagtg cgcgatcgta tgcatcgcgc gagggccagc
     2101 cacgatgact gatatgcagc gcctcgatgc cggcacgacg cgcgcgatcg agcccttcgg
     2161 cctcggccct gttggaaatc acgacgacga tcgaggcatc gagtcgtccg tcgccgaccg
     2221 catcgatgag cgcctgaagg ttgctgccgc ggcccgagat gaggacgccg agccggcgat
     2281 taatggggtc gggagtcatt tttcgaggac agtgcttgtc ccaactgcag gaaggcggca
     2341 ccgcagttgc gttgtacccg gccgtcatgc cgagacgtcc acgcgcatcc acggcgggtt
     2401 tgatctttca cgtcgtcaat cgatccgcga aacgtgcgct tcttttcgag agtccgggtg
     2461 actacgcagc gttcgaacac gtgttggccg ccgcgattgc ccgcagcagc gttgcgcttt
     2521 ttgcgtactg cgtgatgcca aaccactggc atcttctcct atccccgcac gtcgacggtg
     2581 ccctgtcgcg ctgcatgcac tggctgacga ccacccatgc gcgacgctgg cagacggtcc
     2641 gtgggctcga tggtcaggga gcagtctatc aggggcggtt caagtcgatt ccagtcggct
     2701 gcgacgagca cttcttgtgg gtctgccgct acgtcgagcg aaatgccctc cgcgcgtcgc
     2761 tcgtgagccg tgcggaagat tggtcttggt cgagtctcgc ccgccgccat caggatccat
     2821 tgacgtcctg gttgagcgaa tggccagtcc ctcgaccccg ggactggtca gacatcgtga
     2881 accggcccca gaccgatgct gaactgcagg cattcaagac cgccgtagcc aagaacgagc
     2941 cgttcggccg cgacgagtgg aaacgctcga cgatggcgcg aatgggagtg ctgccggcac
     3001 gtcgcggccg gcccctggta gaaaaatgac tcccgacccc attacagata tgtgactgac
     3061 ggttcaccgg cgacgatctc tccgatcacg ggcgccgcca ggacatccgc caacgcgtca
     3121 gcatggtccg gcgccgagac aaggatgagg ccgacgccca tgttgaacgt acgcagcatg
     3181 tcgggatgag gaacgccacc cttctcctgg agccacgtga acacgggagg gacagcccat
     3241 gacgaaggcc tgacgaccgc ggccgtgccg tccggcagga ttcgcggaag gttgtcggta
     3301 atcccgcccc ccgtgatgtg cgccatgcct ttgattcggc cgccggcaag caacggcctg
     3361 atctctggca ggtacgaccg atggggctcg agcaggacgt cgccgatcgt gccgccgagc
     3421 tcgggaacga cgtcgtcgat cgagagcttc aagtgatcga acacgattcg ccgcgcgagc
     3481 gaatacccgt tggtgtgcaa gcccgaggac ggcagtccga tcaggacgtc gcctggcgcg
     3541 atcgtacgcc ccgtgatcag acgctcgcgt tcgacgaccc cgacaatgaa tccggcgatg
     3601 tcgtactccc cgtcggcata gaaacccggc atttcggcgg tctcgcctcc gagcagcgcg
     3661 cagccgttct cgcggcaggc gcgcgccatg cccgagacaa ccgattcggc cacggccggg
     3721 gacaggcgcc cggtcccgaa gtagtcgagg aagaagagcg gccgggcacc ctgcaccaga
     3781 atgtcgttga cgcagtgatt gacgaggtcc acgccaaccg ttccgtgacg tccggccatg
     3841 aacgccacgc gcagcttcgt cccgacaccg tcggcgctcg agaccaggac aggatcgcgt
     3901 gcgagacccg gatcgagacg aaagagccct ccgaaggagc cgatgtccga caggaccgcc
     3961 gaagtgaagg tggacctcgc cagcgcgcgg atgcggtcca cggtctcatt gccggcgtcg
     4021 atgtcgacgc cggcggtctt gtaatccatt acatccacac ctagaactcg agccgctgga
     4081 tcagggtctc ggccagcgtc cgcgccgacg agccctccgc ggcacgccaa tagccggcgc
     4141 gcacggcggc gaagaactgc tcctcggcga agtcctcttc gaggctgtca aaaccgtcct
     4201 tcaacataag gatcgcggcc tctcgcgtcg cctgatccgc agcgtccagc cgcgacacgg
     4261 tccagtccgg ttgtcggagg gcgactccgg cgacggcgct ggccacacgg tcgcgcagcg
     4321 agctctgctt ggcggccagg ttcattaacg cggcaagggc aggctggttc tgggcctgag
     4381 ccaccgcagc cagtgcgtcg atcgccgttc ggatttcgga cggcctggcc ttgggcgcgc
     4441 tcgccgccga gacgagggcg gcaatctgct cgccgcagct ctccccgacc agacagcgtg
     4501 cggccagttt catgagggcc gcttccggcg tcagctccgt cagttctttc agggtctcgg
     4561 cggcgtcgga tccgccgacg cggccaatcg ccagcaccgc atcgtcctgc agcggaccca
     4621 tgagccgggc gacgttgagg atcgcgtcga gcgcgtaggc ggcccggtgg cggccgagcg
     4681 actcgatcac ggcgctgcga aagaagtcga gtccacgttg ggtctcgacg atcatcgggc
     4741 gctgcacggc cgtcgtcgaa ccgagggccc cgagggcgcc gatgagggcc gggcgaacga
     4801 actcggcctg ctcggtgtcg agcgacgcga gcagctgcgt cgccgttctg gggtccgggc
     4861 ggacctcgaa ccacctgtag acgatctcgc gcacgcggtc gctcgggtcc ctgatcatct
     4921 cgcgcatcaa ctcgcgcgtt ccgggatcgt tgaaggaggt cagcacgacg agcgacttgt
     4981 agcggacgaa ctcgttcgtg tgcttccggg ctgcttcggc gagggccggc accgccgcgg
     5041 aggccggcgt tcgacgaatc aactgcgccg cacgcatccg cacgggaaag tcgggatcgc
     5101 ccaggttggc gatgttcttc ggcaggtccg ccgcagtggc gcctgcctgc ccccgggcgt
     5161 acggcggcgc cgccgcgccg accagcagca ccagcgtcac cgcgagcgca gcggcgagca
     5221 cgtcagccga cgacagggtc cctttcaacg tcggggatcg agaacgccag aggttcgggc
     5281 tcctccggct cgggattgag cttgagcgcg agctgcagat acgcctcttc gtcccgtggg
     5341 aaggcgacgg gatagacacc tgtgtagcac gacgtgcaat agctctggct gccgccgttc
     5401 acggcgccgg tcaggccctg cagactcaag tacgacacgc tgtcggcgtc gaggtacttt
     5461 cggatctcct cgatcgtgtg tgtcgccgcg atcagctccg aacggcgcgg cgtatcgacg
     5521 ccatagaagc acggtgaaat cgtcggcggg caactgatgc gcatgtgtac ttcccgcgcg
     5581 ccggcacttc ggaccattcg aacgatcttg cggctggtcg ttccgcgaac gatcgagtcg
     5641 tccacgagca cgacgcgttt gccctcgagg atgctgcgaa ccgggttcag cttcacgcga
     5701 acgccgaaat ggcggatcga ctgctgcggc tcgatgaagg tgcgccccac atagtggttg
     5761 cgaatcaggc ccatgcgcag cggcagaccc gacgcttcgg agtagccgat cgcggcgcac
     5821 acaccggagt ccgggatcgg cacgaccacg tcggcctcga cgctcgactc acgggccagc
     5881 ctgcggccga actccgtgcg gatctcgttg acgctttcgc cgaagacgta gctgtcgggc
     5941 ctcgcgaaat acacgtgctc gaaaatgcac tgcgcgcgcg gagccggcgg gaacggtttc
     6001 agcgacttga ggccgtgcgc ggttacgacg acgatttcgc cgggctcgat gtcgcggacg
     6061 tacgtggccc caatgaggtc cattgcgcac gtctcggaac agatcaccca cgcgtcgccg
     6121 agccggccaa gcgcgagcgg gcggaagccg tgcggatcgc gcgcgccgat cagccggtcc
     6181 ttggtcatca tcacgaacga gtaggcgccg cgcacctgcg acagcgcatc gatgatcgcc
     6241 gtctccgcgc tctcttcacg cgagcgcgca aacaggtgga cgatcacctc ggtgtcggtc
     6301 gttgcctgga agatcgctcc gttcctgacg agctcgtcgc gcagctcggt ggcgttgacg
     6361 atgttgccgt tgtgcccgac ggcaagctga ccgtgggcgc tgtccaccag gatcggctgc
     6421 gcgttggcga tccggctttc cccggccgtc gagtaccgca cgtgaccgac cgccatccgt
     6481 cccggcagct tggcgagagc cgccgtgtcg aaggcatcgt ggacgtagcc catcgccctg
     6541 gacacgcgga taaccgaccc gtcggacgcg gcaatccccg cgctttcctg cccgcgatgc
     6601 tgcagggcat agaggccaag gtacgcgagg ttgctggcct ctgggtggcc gaagattccg
     6661 aagaccccgc actcgtcacg aaacttgtcg aacatcaaca gtcctcaggg ctcaaggccc
     6721 aggcctcaga ggccccaggt tgtagtcgat ctgaggcctg aggcctggtt cctgagccct
     6781 tcgtagtgta cgacgtcatg caatggcctt gcggagttcg aagtacgagg ctatcgagcc
     6841 cgaccagaga cgctcggcct ccatcagcgg ctcgtcgacg acacggcgtc cgtcgacgga
     6901 aatccggatg cgctcgccgc cgacacgacc gattcgcgcg gccgggacgc cggcggccgc
     6961 ggcagcggca agaaggacgg cggccttcga cgggctgacg gacaccacgg ctcgggaggc
     7021 agattcgcca aagagcgtgg cggtgtcgac atagctcgcg gccgcgctca cccccgggac
     7081 gtccacttcg actcccagtg aagaatcaaa gcagcactcg gcgagcgtca cggcgaggcc
     7141 cccctccgag caatcgtgcg ccgagcggat cactcccccc gctgctccct ccacgaggac
     7201 gcgttgaagc gctgcctccc gggagagatc gagggccggt ggcaagccac ggttctgacc
     7261 gtgaacgacg tgaaggaact cgcttccgcc gagctcgtcc cggctgtcgc cgaggagcac
     7321 cacgacgtcg ccttcgtcgc ggaacgccct ggcgacgacg cgatcggcat cttcgatgag
     7381 accgaccatc ccgatgaccg gggtcggtaa cacccctttg ccgtccgtct cgttatagag
     7441 gctgacgttg cctcccgtga tcgggatgtc gagcgcacgg cacgccgcgc ccatgccttc
     7501 gacggcgcgg gcgaactgcc acatgatttc gggcttttcg ggattcccga agttgagaca
     7561 gtttgtcgcg ccgatcggcg ttcctcccgc gcacgccacg tttcgcgcgg cctccgcgac
     7621 ggctagctgc gctcccgcaa acgggtccag gtagacgaag cgtccgttgc agtcggccga
     7681 cagcgcgagg gctcgagacg tacccttcac ccgcacgacg ccagcgccca tgccgggcag
     7741 caccagcgta ttggtgcgga ccatgtgatc gtactgccgg tagacccatt gcttgctcgc
     7801 gatccccgga gaggcgagca gtcgcttgaa caccccgcct ggcgccggcg gcgtccccag
     7861 tttggccaag tcgagccgct gtacgtcggc gatgtaggac ggagtcgcca tcggccggcg
     7921 atacatcggg gcttcgtcgg tcagcgcctt attcggaatg tcggccacga cctgcccgcg
     7981 attcttcact tgcaggcggc caccagtcgt cacttccccg atacggacgg catggaggtc
     8041 ccatttgccg aagatctgct cgacctctgg ctcccgtccc ttcttcacca cgagcaacat
     8101 ccgctcctgc gactcggaga gcatgatctc gtagggcgtc atccccgctt cccgctgcgg
     8161 gacgagcgag acgtcgattt cgacgccggc gcccccgcgc gaacccatct cgcaggtcga
     8221 acaggtcaac cccgccgcac ccatgtcctg gatcccgacg agggctcccg acttcatcac
     8281 ctcgagacac gcctcgagca ggagcttctc catgaaggga tcgccgacct gcaccgccgg
     8341 ccgcttctcc gccgacttct cgtcgaactc ggcggaggcc atcgtggcgc cgtgaatgcc
     8401 gtcccggccg gtcttggcgc ccacgtaata caccgcgttg ccggtgccgg aggccgtccc
     8461 cttgatgatc tcgtcggctc tcgcgatacc gaggcagaac acgttgacca agggattgcc
     8521 ggtatagctc tcgtcgaacc cgatctcgcc gccgattgtc gggatcccga tcgcgttgcc
     8581 gtagccgcca atccccgaca ccacgccggc catcgtgcgg cggacgagcg gatcgtcgag
     8641 ggagccaaag cgaagcgagt cgagaagagc gatgggccgg gcgcccatcg tgaagatgtc
     8701 cctgatgatg ccgccgacgc cggtcgcggc gccttgatac ggctcgatga aggacgggtg
     8761 gttgtgggac tcgatcttga aaaccgcggc gagaccgtcg ccgatgtcca cggcgcccgc
     8821 gttttcgcct ggcccctgca gcacgcgccg tcccgtcgtc gggagcgtct tgagatgcac
     8881 gcgtgagctc ttgtagctgc agtgctctga ccacatcacg gagaagatgc cgagctccgt
     8941 gagattgggt tcgcggccga gcatcgatac gatccgcgcg tactcgtcgc gggtgacacc
     9001 atgccgctgg agtaccgaat ctgtaatggg gtcgggagtc atcggaatga cgccgggttg
     9061 aatttcgcgg aatacgacgc cgggttgaat tttgtggcac cgaaattcaa cccggcgtca
     9121 tttacaaacg cctcgcga
//
LOCUS       DBNGSH010000705         1620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2113092, whole genome shotgun sequence.
ACCESSION   DBNGSH010000705 DBNGSH010000000
VERSION     DBNGSH010000705.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2113092"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="AC16CY_16235"
     CDS             <1..347
                     /locus_tag="AC16CY_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355399.1"
                     /translation="STGAGYWKADVAGAIVYATFQFMQRDATVTHLVWSNIDLVEGHM
                     LQLTNRDKSRTSASIFMHETRLYIAEATVPAGSAEPGLFQQSLGWVDEQGNSIRYRTL
                     YHQGFPTPARAR"
     gene            455..1096
                     /locus_tag="AC16CY_16240"
     CDS             455..1096
                     /locus_tag="AC16CY_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HIR3355400.1"
                     /translation="MRMRFPAAAIAMLSISGLVIAQAPAPKMSFFVTSSNPKGGNLGG
                     LAGADAVCQSLAKGAGAGGKTWRAYLSTSTVDAKSRIGNGPWYNFKGEQIASSVADLH
                     GPNNKISAATALTEKGTAPNYLAGNPPAPVAQPLQHDILTGTNEDGTRNADTCKDWTV
                     GDASAKAMLGHADRIGRNPGVNSWNAIHASQGCGMEQLTPTGGAGMLYCFATN"
     gene            complement(1047..1463)
                     /locus_tag="AC16CY_16245"
     CDS             complement(1047..1463)
                     /locus_tag="AC16CY_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355401.1"
                     /translation="MFTAAGLYNVFWGLWSVFDPQWLFRFTGLPPSNHPAVFACLGMV
                     IGLYGILYFDVARAPERGWLIAAVGLTGKILGPIGLAWLIWTGVWPASAFVLCLTNDL
                     IWWIPFGLYLRDAWPAEVTPLSSSRSNTTSPRRRSG"
BASE COUNT          330 a          515 c          544 g          231 t
ORIGIN      
        1 gctccaccgg cgcgggatac tggaaggccg acgtcgccgg cgccatcgtc tacgcgacgt
       61 ttcagttcat gcagcgcgac gccacggtga cgcacctcgt ctggagcaac atcgacctgg
      121 tcgaagggca catgctgcag ctgaccaaca gggacaagtc ccgtacgtcg gcgtcgatct
      181 tcatgcacga gacgcggctc tacatcgccg aggcgaccgt gccggccggg tccgcggagc
      241 cgggcctgtt tcagcagtct ctcgggtggg ttgacgagca ggggaactcg attcgctatc
      301 ggacgcttta ccaccagggc ttcccgaccc ccgcccgcgc gcgctgaaac gtacgtagtg
      361 tccggcttca gccggaccgc ggggagcacg ccatcggccg ggcccctgca tgtagactag
      421 gcactcccca cttgacgatc caaaggagcc gacgatgcga atgagatttc cagccgcggc
      481 gattgcgatg ctgtcgatta gtggcctggt gatcgcgcaa gcacccgcgc cgaagatgag
      541 cttcttcgtg acgagctcga acccgaaggg cggcaacctc ggcggcctcg ccggggcgga
      601 cgccgtgtgt cagtcgctgg cgaagggcgc aggcgccggc ggcaagacgt ggcgtgccta
      661 cctgagcacg agcaccgttg acgcgaagag ccggatcggc aacggtcctt ggtacaactt
      721 caagggcgag cagatcgcgt cgagcgtcgc cgatttgcac ggcccgaaca acaagatttc
      781 ggcggcgacg gcgctgacgg aaaagggcac tgcgccgaac taccttgccg gcaacccgcc
      841 agcgcccgtc gcgcagccgt tgcagcatga catcctgaca gggaccaacg aggacgggac
      901 caggaacgcg gacacctgca aggactggac ggtcggcgac gcctctgcga aggcgatgct
      961 cggtcacgca gatcgcattg gacgcaaccc cggcgtgaac tcgtggaatg ccatccacgc
     1021 gtcacagggc tgcggcatgg agcaactcac cccgaccggc ggcgcgggga tgttgtattg
     1081 cttcgcgacg aactgagagg tgtcacctcg gccggccacg catcacgcag gtagagaccg
     1141 aacgggatcc accagatcag atcgttggtg aggcacagga cgaacgccga tgccggccag
     1201 acgccggtcc agatcagcca cgccaggccg atggggccga ggattttgcc tgtgaggccg
     1261 acggccgcga tgagccagcc gcgttcggga gctcgcgcca cgtcgaaata caggatcccg
     1321 tagagcccga tcaccatccc caggcacgcg aacaccgccg ggtgattgga cggcggcagt
     1381 ccggtgaacc ggaacagcca ctgcggatcg aacacggacc acaggcccca gaacacgttg
     1441 tagagtccgg cggccgtgaa cacggcgcga tggaatcgtc gtcgagggat caaagtatcc
     1501 tcgaagtgga taatgccatc gagtagatcc ggtcgcgagt gctaccactg caggcagtgg
     1561 gtcgaggaag gcgagccgca cgactgctgg acgacgaccg aagcggcgtt gacgaagggc
//
LOCUS       DBNGSH010000706         5958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3980418, whole genome shotgun sequence.
ACCESSION   DBNGSH010000706 DBNGSH010000000
VERSION     DBNGSH010000706.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3980418"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC16CY_16250"
     CDS             <1..243
                     /locus_tag="AC16CY_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355402.1"
                     /translation="YSAGELKEFSEIAGAARELVKKSYLYTNNHFSAKSVVNAVMLKA
                     QLGEPIEGEYPPELVTQYPELEGLVPTSPPPSRLPA"
     gene            complement(209..778)
                     /locus_tag="AC16CY_16255"
     CDS             complement(209..778)
                     /locus_tag="AC16CY_16255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3355403.1"
                     /translation="MRVLAGVVVALTMVVSASPGVSCQATAPTPRSPGGGPIIVFETS
                     KGSFEVETYPNEAPKTVAHILALVQKNFYNGLRIHRVEPGFVVQWGDPQTRDMTKREL
                     WGTGGSGSAVGVSEVSKKRPHVAGSVGAAFLSDPREADSQIYVTLSPQPQLNSRYTVL
                     GHVISGMDVVTQLRVTDMIKRASVKGAER"
     gene            complement(866..1492)
                     /locus_tag="AC16CY_16260"
     CDS             complement(866..1492)
                     /locus_tag="AC16CY_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR3355404.1"
                     /translation="MRTLAREITGMELPAVEKISEEQAEDPFQILIATLLSARTQDAT
                     THAASTRLFRRARTPRSMSRIPVAEIERLIYPVSFYRNKARHVQACCQMLVSEFGGKV
                     PPTMKDLLRLPGVGRKTANLVLILGFKSLRNICVDTHVHRISNRLGWVRTKDPEETEQ
                     ALYRGTAPRWWPYINLYLVTWGQNVCRPVYPRCGDCVIAGECPRVGVK"
     gene            1564..2934
                     /locus_tag="AC16CY_16265"
     CDS             1564..2934
                     /locus_tag="AC16CY_16265"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3355405.1"
                     /translation="MPILRYLTCVVLVPYAVLAQAQTRPDVDPRIEKLLDGVSEARLG
                     QLVTTLAGFGTRNTLSDTTSSTRGIGAARQWIFEELRRSSPRLQVSFDTYTIARDGRI
                     TRDVELRNVLAVLPGRSARRIYVTAHYDTVNIGGTGQIGANTRAAGQTAADPQLRADQ
                     DYNVDAPGANDNGSGTALTIELARVMATSGLEFDATIVFALWAGEEQGLIGSRAHVQR
                     LAADKADVVANLNSDIVGNSRGGNGIVDGESVRVYSEGPEDSMSRALARYVHRLAALY
                     VPSHRVRLLSRADRFGRSSDHSAFNDRGYASVAFRESNEDFSRQHAATDTVDGVDAAY
                     LARNARVTAASVAALALAPAAPSVNNPAGRPMIGRQPSGYDARLAWNRSEGAVAYRIH
                     WREAWTTDWAQSRLVGDVTEVTLPGLSIDDHIFGVAAVGPDGHESLVSAFVETRARRP
                     DVKLLP"
     gene            complement(2970..3926)
                     /locus_tag="AC16CY_16270"
     CDS             complement(2970..3926)
                     /locus_tag="AC16CY_16270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3355406.1"
                     /translation="MGSLNRPAVLVTRRIPSAVLATLQEACDVDMHDRDDLPHPDLVA
                     RVRGKQGLLSVVTDAIDRSVFEAGSDLKVVSTIAVGYNNIDLAAARERNVIVTNTPDV
                     LTEAAADLTWSLILGMTRRIVEGDRLTRSRAWKGFALDFMLGTDLRGKQLGIVGMGRI
                     GRAVAARAPAFGMRVAFSSRTAQPSPLGESMPLDRLLATSDVVTLHCPLTSETKHLIN
                     QATLARMKRSAYLVNASRGPVVDEAALVWALANRLIAGAALDVYEREPEIHEGLFALE
                     NVVLSPHLGSSTIETRTTMIRLAADNVVAVVTGRPPLTPIPL"
     gene            complement(3914..4144)
                     /locus_tag="AC16CY_16275"
     CDS             complement(3914..4144)
                     /locus_tag="AC16CY_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355407.1"
                     /translation="MNEPELRALVRDIITRQATGAGRGASGSPLQMAGRGRGHASHAM
                     FALPAGADGDGVCLIEPAVTCNHCGYCKSWGH"
     gene            complement(4141..4413)
                     /locus_tag="AC16CY_16280"
     CDS             complement(4141..4413)
                     /locus_tag="AC16CY_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR3355408.1"
                     /translation="MQLARVVGTVVSTQKHPTFEGAKLLLVQPLALDDAPRGTALLAV
                     DSVGAGVHEKVLIVMEGRAAGEALGRKGAPVDAAIVGIVDRVDVNE"
     gene            complement(4437..4703)
                     /locus_tag="AC16CY_16285"
     CDS             complement(4437..4703)
                     /locus_tag="AC16CY_16285"
                     /inference="COORDINATES: protein motif:HMM:NF015289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR3355409.1"
                     /translation="MQLARVVGDVVVTVKNEQLNGIKLMLLQPLAADGTAAGRTLVAV
                     DAVGAGPGETVFFVRGKEAAFPFFPVEVPADAGIVGIVDHWDVG"
     gene            complement(4705..4980)
                     /locus_tag="AC16CY_16290"
     CDS             complement(4705..4980)
                     /locus_tag="AC16CY_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR3355410.1"
                     /translation="MILARIVGTVVATRKDPRLVSSKLLVARPMDPHGKAEGAYIVAV
                     DTVDAGVGETVLIVSGSSARMASGMKDCPVDAAVIGIVDQVDVAGGA"
     gene            complement(4977..5267)
                     /gene="eutM"
                     /locus_tag="AC16CY_16295"
     CDS             complement(4977..5267)
                     /gene="eutM"
                     /locus_tag="AC16CY_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HIR3355411.1"
                     /translation="MGEALGMVETKGLVAMIEAADAMVKAAKVTLVGWEKIGAGYVTA
                     IVRGDVAAVKAATDAGAAAARRVGELVSVHVIPRPHANLEDTLPIGKSAATK"
     gene            complement(5346..>5958)
                     /locus_tag="AC16CY_16300"
     CDS             complement(5346..>5958)
                     /locus_tag="AC16CY_16300"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3355412.1"
                     /translation="VHAHPPIATGFAVAGIPLNRAVLAEVLTTLGSIPIAEYATPSTQ
                     ELPDAVRKYIKAHDGMLLANHGAVTCCRTVTTAYYKMETIEHFARISLVARLLGREHL
                     ISREEVERLQGLRGTYGIAAPAPLCADGSTDAMIDQVLCQVLEAPESASERLVPVVPA
                     PGRVPDAGRAGAGDWPESGDREIRLTYGELTALIRDAVRNLKD"
BASE COUNT          983 a         2063 c         1900 g         1012 t
ORIGIN      
        1 tactccgcgg gcgagttgaa ggagttttcc gagatcgcgg gcgctgcgcg ggagctcgtg
       61 aagaagtcct acctctacac gaacaatcac ttctcggcca agtcggtcgt caacgcggtg
      121 atgttgaagg cgcagctcgg cgagccgatc gagggtgagt acccgccgga actcgtcacg
      181 cagtatccag agctcgaggg gctggtgcct acctctccgc ccccttcacg cttgcccgct
      241 tgatcatgtc cgtgacgcgc aactgggtca cgacatccat tcccgaaatc acgtggccaa
      301 ggaccgtata gcgtgagttc agctgtggct ggggcgacag cgtgacgtag atctggctgt
      361 cggcctcacg cgggtcgctc aagaaggcag cgccaaccga tccagccacg tgaggacgct
      421 tcttgctgac ttcggacacc cccaccgcgc tcccgcttcc gcccgtgccc cagagctcac
      481 gcttggtcat gtctcgcgtc tgcggatcgc cccactggac gacgaaaccc ggctcgacgc
      541 ggtgaatccg aaggccgttg tagaagttct tctgcacgag cgccaggatg tgcgcgaccg
      601 tcttcggcgc ctcgttcggg taggtctcga cctcgaagga acccttcgac gtctcgaaca
      661 cgatgatcgg gccaccgccg ggcgacctcg gcgtcggcgc cgtggcctgg cacgacactc
      721 ccggcgaagc ggacactacc atcgtcagcg cgaccacaac acccgcgaga actctcatgc
      781 tcggcaccct ttcaccctcg caccttcccg ccttcgccgc tcgcggcttc ggcgcggcaa
      841 gcgcaccgtc gcacccccac gcaccctact tcacccccac cctcgggcac tcgccagcga
      901 tgacacaatc cccgcatcga ggatagaccg gccggcagac gttctgaccc cacgtcacga
      961 ggtacaagtt gatgtacggc caccagcgcg gcgctgtacc gcggtagaga gcctgctccg
     1021 tctcctcggg gtccttcgtc cgcacccatc ccagccgatt cgatatccgg tgcacgtgcg
     1081 tgtccacgca gatgttgcgg aggctcttga accccagaat cagcaccaga ttcgccgtct
     1141 tccttccgac gcccggcagc ctcaggaggt ccttcatcgt cggtggcacc ttgccgccga
     1201 attcggacac cagcatctgg cagcacgcct ggacgtggcg cgctttgttt cgatagaagc
     1261 tcaccggata gatcagccgc tcgatctcgg caaccgggat tctcgacatc gacctcgggg
     1321 tgcgcgcgcg cctgaacaac cgcgtggacg ccgcatgggt cgtggcatcc tgcgttcgcg
     1381 ccgacagcag ggtcgcgatg aggatctgaa agggatcttc cgcctgctcc tcggagatct
     1441 tctcgacggc cggcagctcc atgccggtga tctcccgcgc cagcgtgcgc atcactctgc
     1501 cgacaacgcc cctctttggt ctcgcttccc ggctggtgtt catggtcgaa tgtgacaccc
     1561 gagatgccta ttctccggta cctgacctgc gttgtgctcg tgccgtatgc cgtgctcgcg
     1621 caggcgcaga cacgtcccga cgtggacccg cggatcgaga agctcctcga cggggtctcg
     1681 gaagccaggc tgggccaact ggtcaccacg ctggctggct tcgggacgcg caatacgctg
     1741 tccgacacta cgtcgtccac gcgcggcatc ggcgcggcac gccagtggat cttcgaggag
     1801 cttcgccggt cgagtccgag actccaggtc tcgttcgata cctacacgat cgccagggac
     1861 ggccgtatca cacgagacgt ggagctgcgg aacgttctgg ccgtcctgcc cggcaggagc
     1921 gctcgacgga tctatgtgac ggcgcactac gacacggtca acatcggcgg taccggccag
     1981 atcggcgcca atactcgcgc ggcgggccag acggccgccg acccgcagct tcgagccgac
     2041 caggactaca acgtggatgc gcccggcgca aacgacaacg ggagcggcac ggccctgacc
     2101 atcgaactgg cacgggtgat ggccacgagc ggcctcgagt tcgacgccac catcgtcttc
     2161 gcgctttggg caggcgagga gcaggggttg atcgggtcgc gcgcccacgt ccagcgtctg
     2221 gccgcggaca aggccgacgt ggtcgcgaat ctcaacagcg acatcgtcgg caactcgcgc
     2281 ggcggcaacg gtatcgtgga cggggaatcg gtccgggtgt actcggaggg tcctgaggat
     2341 tccatgtcga gagcgttggc ccggtatgtc caccggctcg ccgcgttgta cgtgccgtca
     2401 caccgtgtcc gcctgctgtc gcgcgcggac cgtttcggga ggagcagcga tcactcggcg
     2461 ttcaacgacc gcggctacgc gtcggtggcc tttcgcgagt cgaacgagga cttctccagg
     2521 cagcacgccg ccaccgacac ggtggacggc gtagacgcgg catacctcgc ccggaacgcg
     2581 cgcgtgacgg cagccagcgt cgcggcgctg gcgttggcgc ccgcggcgcc ctcggtcaac
     2641 aacccggcgg gaaggccgat gatcgggcgt caaccctccg gctacgacgc tcgtcttgcc
     2701 tggaaccgtt ccgagggcgc cgtcgcgtac cgaatccatt ggcgggaggc gtggaccact
     2761 gattgggcgc aatcgcgcct ggttggcgat gtgacggagg tcactctgcc aggtctctcc
     2821 atcgacgatc acatcttcgg cgtcgcggcc gttggccccg atgggcacga aagcctcgtg
     2881 agcgcattcg tcgaaacgcg cgcaagacgg ccggacgtga agctgttgcc ctgacggcgt
     2941 tcgtgccgga caccggacac gagcgcgccc tacaacggga tcggggtgag tggcggccgc
     3001 cccgtgacga cggcgacgac gttgtcggcc gccaggcgaa tcatcgtcgt cctcgtctcg
     3061 atggtggagc tgcccaagtg gggcgacagc acgacgttct cgagcgcgaa caggccttcg
     3121 tggatctccg gttcccgttc gtacacgtcg agcgcggcgc cggcgatcag ccggttcgcc
     3181 aacgcccata cgagcgccgc ctcgtccacg accggccctc gagatgcgtt gaccaggtac
     3241 gcgctccgct tcatcctcgc gagcgtcgcc tggttgatca ggtgcttcgt ctctgacgtc
     3301 agtgggcagt gcagcgtcac gacgtccgag gtcgcgagca gccgatcgag cggcatggac
     3361 tcgccaagcg gcgacggctg agcggtccgc gacgagaagg cgacacgcat tccgaacgcg
     3421 ggggctcgcg ccgcaaccgc tcggccgata cggcccatgc cgacgatgcc gagctgcttg
     3481 ccgcgcaggt cggttccgag catgaaatcg agtgcaaatc ctttccacgc ccggctccgc
     3541 gtcagccgat cgccttcgac gatgcggcgg gtcatcccga ggatgagcga ccacgtgaga
     3601 tccgcggccg cctcggtcag gacgtccggc gtgttcgtga cgatcacgtt ccgttcgcgg
     3661 gcggcggcga gatcgatgtt gttgtagccg acggcaatgg tcgataccac cttgagatcg
     3721 ctgccagcct cgaagacgga gcggtcgatc gcatcggtga cgaccgaaag gagcccttgc
     3781 ttgccgcgca cgcgcgcgac gaggtcggga tgcgggagat catcccgatc gtgcatgtcg
     3841 acgtcgcagg cttcctgaag cgtcgcgagc acggccgacg gaattcgacg ggtgacgaga
     3901 acggctggac ggttcagtga ccccacgact tgcagtagcc gcagtgattg cacgtcaccg
     3961 cgggctcgat gaggcacacg ccgtcgccgt cggcgccggc cggcagcgcg aacatcgcgt
     4021 gactcgcatg cccgcgcccc cgaccggcca tctgaagcgg ggagccgctt gccccacggc
     4081 cggcgccggt ggcctgacgc gtgatgatgt cgcgcaccag cgcccgaagc tcgggctcgt
     4141 tcattcgttc acgtccacgc gatcgacgat gccgacgatg gcagcgtcaa ccggcgcgcc
     4201 ctttcggccc aatgcttcgc cggcggcacg gccttccatc acgatgagca ccttttcgtg
     4261 cacgcccgca ccgaccgagt ccactgccag cagcgccgtg cctcgaggcg cgtcatccag
     4321 tgccaggggc tggacgagca gcagcttcgc gccttcgaaa gtcgggtgct tctgcgtcga
     4381 gacgacggtt ccgacgacgc gggcgagctg catgtcttcg ggctccgggc tccgggctac
     4441 ccaacatccc agtgatcgac gatgccgaca atacccgcat cggctggcac ctcgaccggg
     4501 aagaagggaa aggccgcttc cttgccgcgc acgaagaaga ccgtctctcc cgggcccgcg
     4561 ccgacggcat cgacggcgac gagcgtccgg ccggccgccg tgccatccgc cgcgagcggc
     4621 tgcagcagca tcagcttgat cccgttcagc tgttcgttct tcaccgtgac cacgacatcg
     4681 ccgacgaccc tggccagctg catgtcaggc tccacctgcc acgtccacct gatccacgat
     4741 gccgatgacg gccgcgtcca ccgggcagtc tttcatcccc gaggccattc gcgccgagct
     4801 tccgctgacg atgaggaccg tctctccgac gccggcgtcc accgtatcga ccgcgacgat
     4861 gtaggcgcct tcggctttac cgtgagggtc catggggcga gcaacgagca gcttgctgct
     4921 gacgagccgc ggatccttcc gcgtggcgac aaccgtcccg actatgcggg ccagaatcat
     4981 ttagtcgccg cggacttccc gatcgggagc gtgtcctcga ggttggcgtg cggcctcggg
     5041 atgacgtgta ccgacaccag ttcacccacg cggcgggcgg cagcagcgcc agcgtcggtg
     5101 gctgccttga cggccgcgac gtcgccccgg acgatggcgg tcacgtagcc ggcgcctatc
     5161 ttctcccagc cgaccagcgt gactttcgcg gctttgacca tcgcgtcggc cgcctcgatc
     5221 atcgcgacca ggcccttggt ctcgaccatt cccaacgcct cgcccatgct tcactccgtc
     5281 aacttccgac gcccaatttc caacctccaa gcgtctggga gttgggagtt ggcgtgttgg
     5341 aagtttcagt ctttgagatt cctgacggcg tctctgatga gcgccgtgag ttcgccgtat
     5401 gttagccgaa tttctctgtc gccgctctcc ggccagtcgc cagcgcccgc gcgaccggcg
     5461 tccgggaccc ggccgggagc cggcacgacc ggaacgagcc gctccgacgc cgactcagga
     5521 gcctcgagta cctggcacag cacctgatcg atcattgcgt cggtgctgcc gtccgcgcac
     5581 agcggcgcgg gcgcggcaat cccgtacgtg cctcgaagcc cctgaagacg ttcgacttcc
     5641 tcccgggaaa tcagatgctc gcggccaaga agccgggcca ccaggctgat ccgggcgaag
     5701 tgctcgatgg tctccatctt gtagtacgcg gtcgtgaccg tgcggcaaca ggtcacggcc
     5761 ccgtgattgg cgagcagcat gccgtcgtgg gccttgatgt acttccggac cgcatcgggc
     5821 agctcctgcg tcgaaggcgt cgcgtactcc gcgatcggga tgctgcccag cgtggtgagc
     5881 acttccgcca acaccgcgcg gttcagcgga atcccggcca cggcgaaacc ggtcgcgatc
     5941 ggcggatgag cgtggacc
//
LOCUS       DBNGSH010000707         5414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_75424, whole genome shotgun sequence.
ACCESSION   DBNGSH010000707 DBNGSH010000000
VERSION     DBNGSH010000707.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_75424"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="AC16CY_16305"
     CDS             <1..540
                     /locus_tag="AC16CY_16305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3355413.1"
                     /translation="ELVATSPRLAPHFHLPLQHGSDEVLRAMRRPYSAAAYERLVARI
                     RVRVPDASIGSDLIVGFPGESHDHFAETEALLRRLPLTHLHVFPYSDRPGTEAATMPG
                     RVEGATIRERGRVLRSIGQEMAARFRRSQHGTVRRALTVDDGWSAVTDNYLKVKLPQR
                     HERNEWVRVPIDATMSVEQ"
     gene            complement(526..1605)
                     /gene="rlmN"
                     /locus_tag="AC16CY_16310"
     CDS             complement(526..1605)
                     /gene="rlmN"
                     /locus_tag="AC16CY_16310"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR3355414.1"
                     /translation="MTAQSRDLSELELADLERLVTERGFDAFHARQLYRWIHRRAVAD
                     FDEMTDLSRDLRSRLKADFRVDRPRIVDDQVSGDGTRKFVLALADGRKIESVFIPDTP
                     AMTFCVSTQVGCAMSCGFCLTGKMGLVRNLTAGEIAAQVVTLAAEVGLLEQPFNIVLM
                     GMGEPLHNYDNTMKALRMLHAEQGLAVSPRRVTLSTVGIVPGLERLAREPLMPNLAVS
                     LHATTEEQRTALVPPNVKYPLSAIVDACRKFPLKKRSRITFEYVLLDGVNDAPEDARR
                     LARLLSGIKSKINLIPLNPAPGIPFERPSDERVNRFAQILADRHMTVSVRKSRGRDIR
                     AACGQLIVEGGTKKSAAQQMALLLH"
     gene            1785..3056
                     /locus_tag="AC16CY_16315"
     CDS             1785..3056
                     /locus_tag="AC16CY_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3355415.1"
                     /translation="MSQPAIVRDVLPNGLRIVTERMPHVRSVSVGVWLARGSRHESEA
                     QGGIAHFVEHMLFKGTTTRTAEDIAQTVDSIGGQMDAFTAKEYASYYFKVLDEHLPLA
                     VEVLSDIVTNPAFADDDLGREKKVVLEEIKMVEDTPDDLVHELFTENLWPGHPLGRPI
                     LGTKETVESFTPEGLRQYFANVYAAPNLIVSAVGNIEHARVRDLVERAFAGLSETGEQ
                     RREAPPQVVPSTVIRNKDLEQSHVCLGTGSYRQDHAERYSSYVLNTILGGSMSSRLFQ
                     NVREKRGLAYAVFSGLSAYRDAGSMTIYAGCANGTVGELIDVVIGEIRRMKDEPLPHS
                     ELRRAKDHLKGSLMLNLESTSSRMSHLARQEIYFDRQFGLDETLEGVERVTSDDVQRV
                     ARDLFRDSSLAATVLGAVNGLEIPRDRLALG"
     gene            3096..4400
                     /gene="purB"
                     /locus_tag="AC16CY_16320"
     CDS             3096..4400
                     /gene="purB"
                     /locus_tag="AC16CY_16320"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3355416.1"
                     /translation="MIPRYTHPEMGRIWSDQRKYETWLRVEVAAAEAMSRAGIVPAEA
                     ARDIRERGAFEIARIEEIEEATQHDVIAFTTAVAEHVGPSARWLHFGLTSSDVIDTAQ
                     ALQMREACDLILAGLDRLMQAVHARAEEHRRTPMIGRTHGVHAEPMTFGLKLALWYAE
                     LARDAARMTRAREVVAVGKLSGAVGTFAHLPPSVERDVCQTLGLTPAPVASQVIQRDR
                     HAELVSAMAITGSSLEKFALEIRGLQKTEIGEVEEPFGKGQKGSSAMPHKRNPIGCEQ
                     IVGLARLLRANASAAFENNALWHERDISHSSVERVILPDTFIALDHMLRRFTRIVTGM
                     VVYPERMRENLERSRGAVFSGTVLLELARRGVSREQAYEWVQRNAMRSFQEQRDFKAL
                     LLADAEIVHVLSQPDIERAFDLNEQMKHIDHVFERVFQEVAV"
     gene            4400..4660
                     /gene="purS"
                     /locus_tag="AC16CY_16325"
     CDS             4400..4660
                     /gene="purS"
                     /locus_tag="AC16CY_16325"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR3355417.1"
                     /translation="MRALVFVTLKPSVFDPQGTTVAEALHTLGYASVKDVRQGKYFEL
                     DIEAASPDEARRLASDAAAKLLSNPVIESYRIEVDGSTGGAR"
     gene            4657..5412
                     /gene="purQ"
                     /locus_tag="AC16CY_16330"
     CDS             4657..5412
                     /gene="purQ"
                     /locus_tag="AC16CY_16330"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR3355418.1"
                     /translation="MKFAVVVFPGSNCDEDAYHVARHVLGQDAELVWHKDASLRGADV
                     VILPGGFAHGDYLRTGAIARFSPIMRAVTRFASDGGPVLGICNGFQVLLEAGLLPGAM
                     LRNRGLRFRCEFVHVRVEQTDTPFTAACRAGQLLRIPIAHGDGNYYAEPDVIARLERN
                     RQIVFRYVSSAGDPDDEANPNGSVNNIAGICNETRNVVGLMPHPERASEPALGSADGL
                     AVFESILKWGRESFLQDTLDAVVAVPSMSVLKK"
BASE COUNT          946 a         1735 c         1762 g          971 t
ORIGIN      
        1 gaactggtcg cgacgtcgcc ccggctggct ccccacttcc atcttccgtt gcagcacggc
       61 tcggacgagg tcttacgtgc gatgcggagg ccgtactcag cggctgccta cgagaggctc
      121 gtcgcgagaa ttcgcgttcg cgtgcccgat gcctcgatcg ggtcggatct catcgtcgga
      181 tttcccggag agtcgcacga ccacttcgcc gagacggagg ctcttcttcg ccgtctcccg
      241 ctcactcacc tgcacgtgtt cccgtattcg gaccggccgg gaactgaagc cgccacgatg
      301 ccgggaagag tggagggcgc tacgatccgc gaacgcggac gtgttcttcg gtcgattgga
      361 caggagatgg ctgcccggtt ccgccgatcg cagcacggaa ccgtccgccg cgcgctgacc
      421 gtggacgatg ggtggtccgc cgtgaccgac aactatctca aggtgaagtt gccgcagcgt
      481 cacgagcgaa acgagtgggt tcgggtcccg attgacgcga cgatgtcagt ggagcaatag
      541 cgccatctgc tgcgcggccg acttcttcgt cccgccttcg acgatcaact gcccgcacgc
      601 ggcgcgtatg tcgcgccccc ggctttttcg gaccgacacg gtcatgtgac ggtcggccag
      661 aatctgcgcg aatcggttga cgcgctcgtc cgacggccgt tcgaagggaa tccccggtgc
      721 aggattgagc gggatgaggt tgatcttcga cttgatcccg gacagcagcc ttgcgagacg
      781 acgagcgtcc tccggtgcgt cgttgacgcc gtcgaggagc acgtattcga acgtgatgcg
      841 gctgcgcttc ttcagcggaa acttgcggca ggcgtcaacg atcgcagaga ggggatactt
      901 cacgttcggg ggcacgagcg ccgtccgctg ttcctcggtc gtcgcgtgga gcgacacggc
      961 gaggttcggc atgaggggct cccgggccag ccgttccaag ccgggaacga ttccgacggt
     1021 cgacagggtc acgcgccgag gcgagacagc caggccctgt tcggcgtgca gcatgcgcag
     1081 cgccttcatc gtgttgtcgt agttgtgcag cggttcgccc attcccatca gcacgatgtt
     1141 gaacggctgc tcgagcaggc cgacctccgc cgccagcgtc acgacctggg ccgcgatctc
     1201 cccggcggtc agattgcgga ccagtcccat cttcccggtc agacagaacc cgcacgacat
     1261 cgcgcatccg acctgcgtgg acacgcagaa agtcatcgcc ggcgtatcag ggatgaaaac
     1321 cgactcgatc ttgcggccgt cggccagggc gagcacgaac ttgcgcgtgc catcccctga
     1381 aacctgatcg tccacgatgc gcgggcgatc gaccctgaag tccgccttca gacggctccg
     1441 caagtcgcgg gagaggtcgg tcatctcgtc gaaatcggcg acggcgcgtc ggtggatcca
     1501 acggtagagt tgacgcgcgt gaaacgcgtc gaatccccgc tcggtgacga gacgctcgag
     1561 atcggcgagt tccagctcgg agaggtcccg cgactgagcg gtcatggcta tctctcgcgt
     1621 tttcttggag atgcgagcag tcgattgtag cacccttgcc actggaaagg cctgcgtgtt
     1681 atcattcggg cgttcttcta attgcttttc tgtcaatgac ttgcggctgg cgccgcgctc
     1741 cggtagcacg cgttccggta actggccgcc cccaagcgaa tccggtgtct caacctgcaa
     1801 tcgtccgcga cgtgcttccg aatggtctcc gcatcgtcac agagcggatg ccccacgttc
     1861 ggtccgtcag cgtcggcgtc tggctggcgc gcggttcgcg gcacgagtcg gaggcccagg
     1921 gcgggatcgc ccatttcgtg gagcacatgc tcttcaaggg cacgaccacg cggaccgcgg
     1981 aggacatcgc gcagacggtg gattccatcg gcggccagat ggacgcgttc acggccaagg
     2041 agtacgcgag ctactacttc aaggtgctcg acgagcacct gccgctggcg gtcgaggtgc
     2101 tttccgacat cgtcacgaat ccggcgttcg ccgacgacga tctcggccgg gaaaaaaagg
     2161 tcgtcctcga agaaatcaag atggtcgagg acacacccga cgacctcgtt cacgagctgt
     2221 tcaccgagaa cctgtggccg gggcatccgc ttggcaggcc gattctcggt acgaaggaga
     2281 ccgtcgaatc attcaccccg gaggggctgc gccagtactt tgccaacgtc tacgcagcgc
     2341 ccaacctcat cgtgtcggct gtcggcaaca tcgagcacgc gcgcgtgcga gacttggtcg
     2401 aacgggcctt cgccggtttg tccgagacgg gcgagcagcg gcgtgaagcg ccccctcagg
     2461 tcgtgccaag cacggtcatc cgaaacaagg atctcgaaca aagtcacgtg tgtctcggga
     2521 cgggcagcta tcgccaggat cacgccgagc gctattccag ctacgttctc aacacgatcc
     2581 tcggcggttc gatgagctcg cgcctgttcc agaatgtccg cgagaagagg gggctggcct
     2641 acgcggtgtt cagcgggctc agcgcctatc gtgacgccgg atcgatgacg atctacgccg
     2701 gctgcgccaa tggcacggtg ggcgagttga tcgacgtggt gatcggcgaa atccgccgca
     2761 tgaaggacga gcccctgccc cactccgaat tgcggcgggc gaaggatcat ctcaagggca
     2821 gcctcatgct gaacctggag agcacgtcga gccggatgtc gcatctggcg cgtcaggaga
     2881 tttacttcga caggcagttc ggcctggatg agacgctgga aggagtcgag cgcgtgacgt
     2941 ccgacgacgt ccagcgcgtg gcccgggatc tgtttcgaga ctcgtcgctg gctgcaaccg
     3001 tcttgggcgc cgtgaacggt ctggagatcc cgcgggatcg gctggcactc gggtaaggca
     3061 acgcccatgg agggccttcg gatgacctgc ttcagatgat cccccggtat acgcaccccg
     3121 aaatgggtcg tatctggagc gaccagcgga agtacgagac ctggctgcgg gtcgaggtcg
     3181 cagccgccga agccatgtcg cgtgccggca tcgtcccggc cgaggccgcc cgggacatcc
     3241 gggagcgggg ggcgttcgag atcgcccgaa tcgaggagat cgaggaagcc acccagcacg
     3301 acgtcatcgc cttcacgacc gccgtcgccg agcacgtggg tccttccgcc cggtggctcc
     3361 acttcggcct cacatcgtcc gacgtcatcg acacggccca ggccctccag atgcgcgagg
     3421 cctgcgacct gatcctggcc gggctcgatc ggctgatgca ggccgtccac gcgcgtgccg
     3481 aagagcaccg ccgcacgccg atgatcggga ggacgcacgg cgtccacgcc gagcccatga
     3541 ccttcggcct caagctcgcg ctttggtacg ccgagctggc gcgcgatgca gcccgcatga
     3601 cgcgtgcgcg cgaggtcgtc gcggtcggca agctgtctgg cgccgtcggc acgttcgccc
     3661 accttcctcc gtccgtcgag cgcgatgtct gtcagaccct cggcctgact ccagcgccgg
     3721 tcgcctcgca ggtcattcag cgcgaccgcc atgccgagct cgtctcggcg atggctatta
     3781 ccggttcatc gctcgagaaa ttcgcgctcg agatccgtgg gctgcagaag acggagattg
     3841 gcgaagtcga agagccgttt ggcaaggggc agaagggctc gtcggccatg ccgcacaagc
     3901 ggaacccgat cggctgcgag cagatcgtcg gattggcccg gctgctgcgc gcaaatgcct
     3961 ccgcggcgtt cgagaacaac gccttgtggc acgagcgcga catctcgcat tcctcggtcg
     4021 agcgtgtcat tctgcccgac accttcatcg cgctcgatca catgcttcgc aggttcacac
     4081 ggatcgtaac gggaatggtc gtgtaccccg agcgcatgcg ggagaatctc gagcgctcgc
     4141 gcggagcggt gttctctggt actgtgctgc tcgaactggc cagacgcggc gtgtcgcgcg
     4201 aacaggccta cgagtgggtg cagcgcaacg cgatgcgttc gttccaggaa cagcgtgatt
     4261 tcaaggcact gctgcttgct gacgccgaga tcgttcacgt gctgtcccag ccggatatcg
     4321 agcgggcctt cgacctgaac gagcagatga agcatatcga ccacgttttc gaacgggtct
     4381 tccaggaggt agccgtctga tgcgggctct cgtgttcgtg accctcaagc cttcggtgtt
     4441 cgatccgcag ggaacgaccg tcgccgaagc gctccacacg ctcggctatg cctcggtgaa
     4501 ggacgtccgg caaggcaagt acttcgagct cgacatcgag gcggcgagcc cggacgaggc
     4561 ccggcggctg gcctcggacg ccgccgccaa actgctctcc aaccctgtca tcgagagtta
     4621 tcgcatcgag gtggacggca gcacgggagg cgcgcgatga agttcgccgt tgtcgtgttc
     4681 cccgggtcca actgcgacga ggacgcgtac cacgttgcgc ggcacgtgct cggccaggac
     4741 gccgagctcg tctggcacaa ggacgccagc cttcgcggcg ccgatgtcgt catcctgcct
     4801 ggcggcttcg cgcatggcga ttatctgcgc accggcgcca tcgccaggtt ttcaccgatc
     4861 atgcgcgcgg tgaccaggtt tgcctccgat ggaggaccgg tcctcggcat ctgcaacggc
     4921 ttccaggtgc tgctcgaggc aggcttgctg ccgggcgcca tgttgcggaa ccggggactg
     4981 cgcttccgct gcgaattcgt gcacgtgcgc gtggagcaga ccgacacacc gtttaccgct
     5041 gcgtgcaggg cagggcagct cctgcggata ccgattgcgc acggggacgg gaactactac
     5101 gcggagcccg acgtgatcgc ccgcctcgaa cgcaaccggc agatcgtctt tcgctacgtc
     5161 tcgtctgccg gcgatccgga tgacgaggcc aatccaaacg gatccgtcaa caacatcgcc
     5221 ggcatttgca acgaaacgcg caacgtcgtg gggctcatgc cgcatcccga acgggcgtcc
     5281 gagccggccc ttggcagcgc cgacggcctg gccgtattcg agtcgatcct taaatggggt
     5341 cgggagtcat ttcttcagga caccctcgac gccgtggtgg cagtgccttc gatgagcgtc
     5401 ctgaagaaat gact
//
LOCUS       DBNGSH010000708         3033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2622708, whole genome shotgun sequence.
ACCESSION   DBNGSH010000708 DBNGSH010000000
VERSION     DBNGSH010000708.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2622708"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /gene="cysS"
                     /locus_tag="AC16CY_16335"
     CDS             complement(<1..1005)
                     /gene="cysS"
                     /locus_tag="AC16CY_16335"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR3355419.1"
                     /translation="MLRLYNTLTRQEEPFAPLRDNTVRMYACGLTVYARGHIGNFRTF
                     VCLDVLRRALKYLCGYRVSEVINYTDVDDKTIAGAEKAGLPLREYTAQWIDAFRSDAG
                     LLGIETPEHTPRATDEPNMRAMCDMIQALERNGHTYRRDGSVYFKIDSLPSYGRLARL
                     DREGLHAGARVDVDEYSKDDPRDFVLWKASKPGEPSWDCAGLPGRPGWHIECSAMALR
                     LLDGGPIDIHAGGVDLIFPHHENEIAQSEGATRDAFSRFWVHVEHLFVENEKMSKSVG
                     NVFTIPDIVQKGYRPSALRYLLLSSHYRKQLNFTWAGMEQAEESLRRIADFLVRLDGV
                     A"
     gene            complement(1015..1278)
                     /locus_tag="AC16CY_16340"
     CDS             complement(1015..1278)
                     /locus_tag="AC16CY_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355420.1"
                     /translation="MDASRFVFSLSVPSDVKYVPTVRRLAVQAARYASCADADAESFG
                     GSVEDAFRSCLQEPSDGVISVVVRRDAGPVEVRVNGHAVTVEP"
     gene            complement(1280..2215)
                     /locus_tag="AC16CY_16345"
     CDS             complement(1280..2215)
                     /locus_tag="AC16CY_16345"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR3355421.1"
                     /translation="METIHFPDDPRPPWLMHPVLALGNFDGLHRGHLKIVERVRRGAA
                     EHGGTPMAMTFDPHPPRVVRPEKAPPLLMTTAQRLEALHGAGIGCVAVVRFTRELSEW
                     DPETFVRTALVEWLHVSEVWVGANFLFGHGRSGTFTVLRTLGQRYGFRADKIDPVRYK
                     DFVVSSTRIRRLVSEGRVDEAGALLGHPYFIDGTVVHGQRRGHEIGFPTANLTTDNEL
                     IPPHGVYATTTTIDGVVHAGLTNIGVRPTFGESVPTVETHLLRYTGDLYGRRVRLGFV
                     QRVRDERRFPDVDALRRQIETDRREADRLFSRFSV"
     gene            complement(2216..>3033)
                     /locus_tag="AC16CY_16350"
     CDS             complement(2216..>3033)
                     /locus_tag="AC16CY_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355422.1"
                     /translation="ERSRGVMSRQVQARVVFLGTGTSHGVPMIGCDCAVCRSEDPRDK
                     RLRPSVYVDVPSYARLLIDAGPDLRQQALVNHVTHVDAILLTHAHADHILGLDEVRRF
                     NVLRGSSIPCYASESTWQTVWKTFHYIFDGVPRIGGGIPKLDPHEIRGPLAIQGVSVV
                     PVPLWHGEMPVLGFRFGGFAYLTDCNRIDESSMALVSGVDTLVIDALRDRPHVTHFSV
                     SEALEVIARLKPRRAYMTHMTHDLGHEATSARLPEGVHLAYDGLVLDVRVDVE"
BASE COUNT          504 a         1049 c         1009 g          471 t
ORIGIN      
        1 ggcaactccg tccagcctca ccaggaagtc ggcgatgcgc cgcagcgact cctcggcctg
       61 ctccatcccg gcccacgtga agttcaactg cttccggtag tgcgacgaca gcagcaggta
      121 ccggagcgca gacggacgat agcccttctg gacgatgtcg gggatggtga agacgttgcc
      181 gaccgacttc gacatcttct cgttctcgac gaagaggtgc tcgacgtgca cccagaaccg
      241 cgagaacgcg tccctggttg ccccctccga ctgcgcgatt tcgttctcgt ggtgcggaaa
      301 aatcaggtcc acgccgccag catggatgtc gatcggcccg ccatcgagca gacggagggc
      361 catggccgag cactcgatat gccagcccgg gcggccaggg agcccggcgc agtcccacga
      421 cggctcgcct ggcttggacg ccttccagag cacgaaatcg cggggatcgt ccttcgagta
      481 ctcgtccacg tccacccgcg cgcccgcgtg cagcccctcg cgatcgagcc gcgccaggcg
      541 tccgtaggac ggcagcgagt cgatcttgaa gtagaccgac ccgtcgcgcc ggtaggtgtg
      601 gccgttccgc tcgagcgcct gaatcatgtc gcacatcgct cgcatgttcg gctcgtcggt
      661 cgcgcgcggc gtgtgctccg gcgtctcgat gccgagcagc ccggcgtccg accggaacgc
      721 gtcgatccac tgcgcggtgt actctcgcag cggcagcccg gctttctccg cgccggcgat
      781 cgtcttgtcg tcgacgtccg tgtagttgat cacctcggag acgcgatacc cgcacaggta
      841 cttcagcgcc cggcgcagca cgtcgaggca gacgaaggtc cggaagttgc cgatgtggcc
      901 gcgcgcgtag acggtcagcc cgcacgcgta catccgcacg gtgttgtcac gcagcggagc
      961 gaacggttcc tcttgccggg tcagcgtgtt gtagaggcga agcatcccgg aacgtcaggg
     1021 ctccacggtc acggcatggc cgttcacgcg gacctccacc ggcccggcgt cgcggcgcac
     1081 gacaacggag atgacgccgt cggagggctc ctgcaggcac gaacggaagg cgtcctcgac
     1141 cgagccgccg aacgactcgg cgtccgcgtc ggcgcagctc gcatagcggg ccgcctgcac
     1201 ggccagcctg cggacggtgg gcacgtactt cacgtcgctg ggcaccgaga ggctgaagac
     1261 gaatcggctg gcgtccacgt tacacgctga accgactgaa gaggcgatcg gcctcgcgcc
     1321 gatccgtctc gatttgccgc cgcagcgcgt cgacatccgg gaagcgccgc tcgtcccgga
     1381 cgcgctgcac gaatcccagc cgcacgcgcc ggccgtacag gtcgccggtg taccgcagga
     1441 gatgggtttc gacggtcggc accgactccc cgaaggtagg cctcacgccg atgttggtca
     1501 ggcctgcgtg cacgacgccg tcgatggtgg tggtggtggc atagaccccg tgcggaggaa
     1561 tcagctcgtt gtcggtcgtc aggttcgcgg tcgggaagcc aatctcgtgc ccgcgccgct
     1621 gcccgtgcac gacggtgccg tcgatgaagt aggggtggcc gaggagggcg cctgcctcgt
     1681 ccacgcgccc ctcggagacg aggcgccgga tccgcgtgct gctcacgacg aagtccttgt
     1741 agcgtaccgg gtcgatcttg tcagcacgaa aaccgtaacg ctgcccgagc gtccggagca
     1801 ccgtgaacgt gccgctgcgc ccgtgcccga agaggaagtt tgcaccgacc cacacctccg
     1861 agacgtgaag ccactcgacg agcgccgtcc gcacgaaagt ctccgggtcc cattccgaca
     1921 actcgcgcgt gaaccgcacg accgccacgc acccgatgcc ggcgccatgg agcgcctcga
     1981 gccgctgcgc cgtcgtcatc aggagcggcg gcgccttctc cggccgcacc accctcggcg
     2041 gatgcggatc gaacgtcatc gccatcggcg tgccgccgtg ctcggcggcg cctcgcctga
     2101 cgcgctcgac gatcttcagg tgaccgcgat gcaagccgtc gaagttgccc aacgccagca
     2161 cgggatgcat gagccacggc ggcctggggt cgtccgggaa gtggatggtc tccattcact
     2221 ccacgtcgac tcgcacgtcg agcacgagcc catcatatgc caggtggacg ccttcgggca
     2281 ggcgcgcgct cgtcgcctcg tgcccgaggt cgtgcgtcat gtgggtcatg taggcgcgcc
     2341 gcggcttcag ccgcgcaatc acctcgagcg cctccgacac gctgaagtgc gtcacgtgcg
     2401 gccgatcgcg caaggcgtcg atgacgagcg tgtcaactcc cgacacgagc gccatcgagg
     2461 attcgtcgat gcggttgcag tcggtgaggt acgcgaaacc gccgaaccgg aatccgagga
     2521 ccggcatctc gccgtgccac agcggcactg ggacgaccga gacgccctgg atcgcgagcg
     2581 gtccgcgaat ctcgtgcggg tcgagcttcg ggattccgcc gccgatccgt ggcacgccgt
     2641 cgaagatgta gtgaaacgtc ttccacaccg tctgccacgt cgattcgctc gcataacacg
     2701 ggatcgacga gccgcgcagg acgttgaagc ggcggacctc gtcgaggccg aggatgtgat
     2761 cggcatgggc gtgcgtcagc aggatcgcgt cgacgtgcgt cacgtggttg acgagcgcct
     2821 gctgccgcag gtcaggcccg gcgtcgatca ggagcctggc gtacgacggc acgtccacgt
     2881 agaccgaggg gcggagccgc ttgtcgcgcg ggtcttccga tcggcacacc gcgcagtcgc
     2941 acccaatcat cggcacgccg tgcgacgtac ccgtgccgag gaagaccacc cgggcctgga
     3001 cctgccgaga catcacccct cgactccgct cgg
//
LOCUS       DBNGSH010000709         2002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4320680, whole genome shotgun sequence.
ACCESSION   DBNGSH010000709 DBNGSH010000000
VERSION     DBNGSH010000709.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4320680"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="AC16CY_16355"
     CDS             complement(<1..953)
                     /locus_tag="AC16CY_16355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3355423.1"
                     /translation="MRFRSLSSATGIVALLLSVTAGAQQLAQSERDQIDRDALAVLQA
                     TGAPSASIAIVRGKEIVYERAYGDARIDPRTPASPSMRYAIGSVSKQFTAAAVLLLEQ
                     DGQLSLNDTVARWFPQLTRANEISVRQLLSMTSGYQDYWPQDYVFPDMRKPSTAQAIM
                     QRWAAKPLDFEPGAKWQYSNTNYVIAAAIVERVSGMPFMQFLRARIFAPLKMTSVADF
                     DTGPLSAADAAPYVRNGLGPLRPAPKEATGWLFGAGQLAMTARDLALWNLSVINRSIL
                     RPESYRSQQAEALLQAGTATGYGLGVAVGVQDGRRRVSHGGA"
     gene            complement(987..>2002)
                     /locus_tag="AC16CY_16360"
     CDS             complement(987..>2002)
                     /locus_tag="AC16CY_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3355424.1"
                     /translation="DFYKETMRRITELPGVERVAIGTLVPWREAGNFGSGFQFTAEGY
                     ARANGEEDPRARFRTVSSGFFSALGVPLIAGRDFNDADRSGAERVVIVSQSLAQRMFP
                     NQDAVNRRIMWTDPVMKFIPVSPEPRRVVGVVADVDDENVVPGPAVTVYHPFGQEIGG
                     GRLFIHARTDPYALVPAVTRIIRDLAAEQPVERAATLEDVRAEVLAPQRLNALVFGGF
                     ASVALAIAIVGVAGVLAFSVSARTREFGIRLAIGSEPKSLLAGVLKEGALISATGVAA
                     GAIGGFAFARLAGSYIEDVTIPGPLTIAGAAMVLVAAAVLASIVPAARASRVDVVDAL
                     RPE"
BASE COUNT          332 a          682 c          684 g          304 t
ORIGIN      
        1 gcgccgccgt gggagacgcg tcgccggccg tcctgaacac ctacggcgac gccgagccca
       61 taaccggtcg cggttccggc ctggagcagg gcctccgcct gctgcgatcg atacgactcc
      121 ggccgcagga tggaccggtt gatgaccgag agattccaca gcgcgaggtc acgtgcggtc
      181 atggcgagct gtccggcgcc gaagagccat cccgtcgctt ccttcggagc cggccgcaag
      241 gggcccaggc cgttgcgcac gtacggggcg gcgtctgccg cgctcaacgg acctgtatcg
      301 aagtccgcca ccgacgtcat cttcagcgga gcgaagatcc gggcgcgcag gaattgcatg
      361 aagggcatcc cggacacgcg ctcgacgatt gccgcggcga tgacgtagtt ggtgttgctg
      421 tactgccact tggcgccggg ctcgaagtcc aggggcttcg ccgcccaccg ctgcatgatc
      481 gcctgggcgg tcgagggttt ccgcatgtca gggaagacgt agtcctgcgg ccagtagtcc
      541 tggtatcccg acgtcatcga cagcagctgc ctcacgctga tctcgttcgc gcgcgtgagt
      601 tgcgggaacc atctggcgac cgtgtcgttc aacgacagtt ggccgtcctg ctcgagaagc
      661 agcaccgcgg cggcggtgaa ctgtttgctg acggagccga tcgcgtagcg catcgacggc
      721 gaggcgggcg tcctgggatc gatgcgcgca tcgccgtacg cgcgctcgta cacgatctct
      781 ttccctcgca cgatggcaat cgaggcgctg ggcgcgcccg ttgcctggag taccgcaagg
      841 gcgtcgcggt caatctgatc gcgttcggat tgcgcgagct gttgggcgcc ggcggtgacg
      901 gacagaagaa gggcgacgat gcccgtagcg ctcgagaggg agcggaatct catgctggac
      961 actgtctctc ctgatgcctc ttctgactac tcaggccgca gcgcgtccac gacgtccacg
     1021 cgcgaggcgc gcgccgccgg cacgatcgac gccaggactg cggccgccac caggaccatc
     1081 gcggccccgg caatcgtcaa gggaccggga atggtgacgt cctcgatgta gctgccggcc
     1141 aggcgcgcga acgcgaaccc gccaatcgct ccggcggcaa cgcccgtcgc cgaaatgagc
     1201 gcgccttcct tgagcacgcc cgccagcagg ctcttcggct cggacccgat ggcgaggcgg
     1261 atgccgaact cacgtgtacg ggcgctcacg gaaaacgcca ggacgccggc cacgcccacg
     1321 atggcgatcg ccagcgccac ggaggcaaac ccgccgaaca ccagcgcgtt cagtcgctgc
     1381 ggggcgagta cttcggcgcg cacgtcttcg agcgtcgcgg cccgctccac gggctgctcc
     1441 gcggcgagat cgcgaatgat gcgtgtgacg gcaggaacga gcgcgtacgg atccgtccgt
     1501 gcgtggatga aaaggcgtcc gccgccgatc tcctgcccga aggggtgata gacggtcacc
     1561 gccggccccg gcaccacgtt ctcgtcgtcc acgtccgcaa cgacgccgac gacgcggcgg
     1621 ggttcagggc tgacggggat gaacttcatg accgggtcgg tccacatgat gcggcggttc
     1681 accgcatcct ggttgggaaa catccgctgc gccaggctct ggctgacgat gacgacgcgc
     1741 tcggcgccgc tgcgatcggc gtcgttgaaa tcgcgccccg cgatcagcgg cacgccaagg
     1801 gcggagaaaa accccgagga cacggtgcgg aaccgcgcgc gcggatcctc ctcgccgttg
     1861 gcgcgcgcgt acccctccgc ggtgaactgg aagccggacc cgaagttccc ggcctcgcgc
     1921 cacggcacca gcgtgccgat agccacccgc tccacgccgg gcagctcggt gatgcggcgc
     1981 atcgtctcct tgtagaagtc ga
//
LOCUS       DBNGSH010000710         2522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_585785, whole genome shotgun sequence.
ACCESSION   DBNGSH010000710 DBNGSH010000000
VERSION     DBNGSH010000710.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_585785"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1624
                     /locus_tag="AC16CY_16365"
     CDS             185..1624
                     /locus_tag="AC16CY_16365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HIR3355425.1"
                     /translation="MPLYVDDIQGILLHGYGHLKHAAFLLLGITEPGAARAWLSTLDV
                     RNADFTRDEVDQSVNIAFTAPGLTRLGLDEAVLGEFSTEFREGVAGTAHRQRILGDSG
                     DSSPERWRWGGPRNPQPHVLLMLYARDGRALSALVNEHHARLATAGLEAIERLDTTWL
                     PHDKEHFGFRDGVSRTGIDGFHRNTAPIDTIAPGEFVLGHRNAYGQYTHRPLLGREQD
                     PHRILADAPDEPGRRDLGMNGSYLVFRQLSQDVATFWQFCADKTRRTDGTSSPADRIR
                     LASKMVGRWPGGAPLVKSPDADDPTLGRDNDFLYHGSGDAHGLKCPIGAHIRRTNPRD
                     SLEPDPGSERSIDVGKRHRIIRRGRAYGPPAAASMDIDDILAAGDAPGERGLHFLCFN
                     THIGRQFEFIQHTWLNNPGFDGLYDDDDPIAGDRGSSHGKPAGTFTIQADPVRTRVTG
                     IPRFVQVRGGGYFFLPGIRALRFLASLKT"
     gene            1728..1931
                     /locus_tag="AC16CY_16370"
     CDS             1728..1931
                     /locus_tag="AC16CY_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3355426.1"
                     /translation="MKGTIRTIRADKGFGFIKDDAGKEYFFHRSAVYGEGLENLREGD
                     GVEFDIGEGPKGPRAENVRRTTT"
     gene            1976..>2522
                     /locus_tag="AC16CY_16375"
     CDS             1976..>2522
                     /locus_tag="AC16CY_16375"
                     /inference="COORDINATES: protein motif:HMM:NF040142.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase CrtO family protein"
                     /protein_id="HIR3355427.1"
                     /translation="MLSAARQILAFVVATGVVIAVVDALSRTAVFPMFRYPGGAVLVY
                     GLSMTLVWLMRRLAPFALPHRYHRLRPFEIDGRLYRGLGIASFNWLLRKSGIELLNFS
                     ARLSHGRAGLASLERGIRDAETDHAIALLAMAAVTLYAAANSWWAFTGWLLLANVVAN
                     VYPIMLQRHNRARLLPVLQRRR"
BASE COUNT          440 a          806 c          832 g          444 t
ORIGIN      
        1 cgaagaacat cgccaacaac gcggccattc gcctggggct gtcgggccgc atgaacgaag
       61 acgagacgag ggcgtggctg cgccgattcg gcgccggcaa ccagttgccc gaatcgggag
      121 tcgtcgcgcg gctcttcgac ggcatccgct gggacaggct gtgccgctgc aagtagctga
      181 ggttgtgccg ctatacgtag acgacattca aggcatcctg ctgcacggct acggccactt
      241 gaagcatgcg gcgttcctgc tgctcggcat cacggaacca ggggctgcca gggcgtggct
      301 ctccacgctc gacgttcgca acgcggactt cacgcgcgac gaagtcgatc aatccgtcaa
      361 catcgcgttc accgcgcccg gactgacccg cctcggcctg gacgaggcag tgctcggcga
      421 gttctctacg gaattccgcg aaggtgtcgc ggggaccgcg caccggcagc gaatcctcgg
      481 cgattccggc gacagcagcc ccgaacgatg gcggtggggc ggcccacgca acccgcagcc
      541 gcacgtcctg ctgatgctct acgctcgcga cggacgtgcg ctgtctgcgc tggtgaatga
      601 gcaccatgca cggctggcca ccgcgggtct cgaggcgatt gaacggctgg acaccacctg
      661 gctgcctcac gacaaggagc atttcggttt tcgcgacggc gtgagccgga cggggatcga
      721 cgggttccac cggaataccg ccccgatcga cacgatcgcg ccgggcgagt tcgtattggg
      781 gcatcgcaac gcgtacgggc aatacacgca tcgccccctg ctcggccgcg agcaggatcc
      841 ccatcgcatt ctggccgacg cgccggacga accggggcgc cgcgacctgg ggatgaacgg
      901 cagctatctc gtgtttcgtc agctcagcca ggatgtggcg acgttctggc agttctgcgc
      961 ggacaagaca cggcgcaccg acggaacgtc gagtccggcg gaccggatac ggctcgcctc
     1021 gaagatggtc ggccgctggc ctgggggtgc gccgctcgtc aagtcgccgg acgctgacga
     1081 tcccacgctc ggcagggaca acgactttct ctatcacggc tccggggacg cacacggctt
     1141 gaagtgcccg atcggggccc acatccgccg caccaacccg cgcgactccc tggagccaga
     1201 cccgggctcg gagcgatcga ttgacgtggg taagcgccac cggatcatca ggcgcggacg
     1261 tgcctacggc ccgccggcgg ccgcgtcgat ggacatcgac gacatcctcg cggccggcga
     1321 cgcgccgggt gaacgcggac tccacttcct gtgcttcaac acgcacatcg gccggcagtt
     1381 cgagttcatc cagcacacgt ggctgaacaa cccggggttc gacggcctct acgacgacga
     1441 cgatcccatc gccggcgacc gtggaagctc gcacggaaag cccgcgggca ctttcaccat
     1501 ccaggcggac cctgtccgca cgcgcgtgac ggggatcccg cgattcgtgc aggtcagagg
     1561 cggagggtat ttcttcctgc cggggattcg tgcactgcga ttccttgcgt cgctgaagac
     1621 gtagggcgtg gtcggatgtt tgcacgttag aattgcgggc gtgcgctgcg cccaccgcgg
     1681 gtattgggat ttctgaacgt cgatacggag taggagagga tgacacgatg aagggaacga
     1741 ttcgaacgat acgagctgac aagggattcg gcttcatcaa ggacgatgcg gggaaggagt
     1801 acttctttca ccgcagcgcc gtgtatggcg aaggtctcga gaatctcaga gagggcgacg
     1861 gcgtggaatt cgacattggc gagggcccga aaggtccgcg ggccgagaac gtcaggcgca
     1921 cgacgactta atcggaccac cgtcgattga tcgttccggt cgggagtctc gaccagtgtt
     1981 gagcgcggcc cgacagatac tggcgttcgt cgtcgcgact ggagtcgtga ttgccgtcgt
     2041 ggatgccctt tcgcgcacgg ccgtcttccc catgttccgc tatcccggtg gcgcggtcct
     2101 ggtttacggc ctgtcgatga cgctggtctg gctcatgcgg cggttggctc ccttcgccct
     2161 tccccaccga taccatcgtc tgcggccttt cgaaatcgac ggccgtctgt atcgaggtct
     2221 cggcatcgcc tcgttcaatt ggctgctccg gaagagtggt atcgagctcc tgaatttctc
     2281 ggccaggctg tcacacggac gagcgggact cgcgagcctc gagcgcggca tccgcgacgc
     2341 cgaaacagac catgcgattg cccttctggc gatggcggcg gtcactctct acgccgccgc
     2401 gaattcctgg tgggcgttca cgggttggtt gttgcttgcc aacgtcgtgg ccaacgtgta
     2461 tcccatcatg ctgcagcggc acaatcgggc gcgactgtta ccagtgctgc agagacggcg
     2521 cc
//
LOCUS       DBNGSH010000711         2001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2113920, whole genome shotgun sequence.
ACCESSION   DBNGSH010000711 DBNGSH010000000
VERSION     DBNGSH010000711.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2113920"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="AC16CY_16380"
     CDS             <1..680
                     /locus_tag="AC16CY_16380"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3355428.1"
                     /translation="DAIGKRFVFSGPRTVVGIVDDARVSNVTTVPGLQVYLPIQEQPQ
                     SYLSLVARGASAEDVRALLPHINAAVHQVNPTLPTYSAQPMEAVVGATIAPRRLNTLL
                     ISVFGAVALCLAAIGVYGVLAYSVAQRTREIGLRMALGAQRTWVLGLVVRQGLRLAVM
                     GIGLGVVAAVATTRVLQVMLHGVTPRDPATFTAVVLLFAVVAGLASFISGWRATKVDP
                     LNSLRCE"
     gene            741..1709
                     /locus_tag="AC16CY_16385"
     CDS             741..1709
                     /locus_tag="AC16CY_16385"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355429.1"
                     /translation="MFFRGQTIGKYRIESSLGSGGFGVVYLAEDTWIKKKVALKVPHK
                     QQLDFSELLKEPRLLASLSHPNIVTVLTAERQEKVFFIVMEYVRGETLEHLIMREGAL
                     DLSRAQDFSCQICNAVDHAHRAGVLHRDLRPGNMLISDAGMLKVTDFGTSRFLEIAAH
                     GTTIIGSPAYMAPEQFHGKAVFASDIYSVGVTMYQMLTGSLPYDTPAPADIGKLERGE
                     LVSAPRLKNPKIPKALNDIVLRALAPDPSARYQRVADLLEDLLAARETAPRRAVRRGS
                     ASANATVAEIHSRLKARETRPARFCWHCRKPLHARTNKCPFCGETQ"
     gene            1739..>2001
                     /locus_tag="AC16CY_16390"
     CDS             1739..>2001
                     /locus_tag="AC16CY_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3355430.1"
                     /translation="MQVLLLSMPDSFEHTPTLAVRMPNGGLASLAGNVDTHHRVAIAD
                     LILAQSAVQPTVIRLMNELRPDLVGLSVMTFQRGTARRIISLV"
BASE COUNT          330 a          683 c          632 g          356 t
ORIGIN      
        1 aggatgcgat cggcaagcgc ttcgtcttca gcggaccgcg gaccgtagtc ggcatcgtcg
       61 acgatgcccg cgtatcgaat gtgacgacgg tgccggggct ccaggtgtac ctgcccatcc
      121 aggagcagcc ccagagttac ctctccctcg tggcacgcgg cgcgagtgcg gaagacgtgc
      181 gcgcgttgct gccgcacatc aacgcagctg tgcaccaggt caatccgacg cttcccacgt
      241 attccgccca gccgatggaa gccgtggtgg gcgcgaccat cgcgccccgt aggctcaaca
      301 cgctgctcat cagcgtcttc ggggcggtcg cgctctgtct ggccgcgata ggggtgtatg
      361 gagtcctggc ctattccgtg gcccaacgaa ctcgtgaaat cggcctccgc atggcgctgg
      421 gcgcacagag gacgtgggta ctcgggctcg tggttcggca ggggctgcgc ctggccgtga
      481 tgggcatcgg gcttggcgtg gtggccgccg ttgccacgac gcgcgttctc caggtgatgc
      541 tccacggagt cacgccgcgg gaccctgcca cgttcactgc tgtcgtactg ctctttgcgg
      601 tggtggccgg actcgcgtcg ttcatatcgg gctggcgggc aacgaaggtc gatcccctca
      661 actcacttcg atgtgagtag ctgtatagcc gctgcaccgc ttcgacgcgg cgagccctgc
      721 tatactccct ccacccctcg atgttcttcc gcggccagac catcggcaag taccgcatcg
      781 aatcatctct tggcagtggt ggctttggcg tcgtctacct cgccgaggac acctggatca
      841 agaaaaaggt cgcgctgaag gttccgcaca agcagcagct cgacttcagc gagctgctca
      901 aggagccgcg gctcctcgcc tccctgagcc atccgaacat cgtcaccgtc ctcaccgccg
      961 agaggcagga gaaggtgttc ttcatcgtga tggagtacgt gcggggcgag acgctggaac
     1021 atctgatcat gcgcgagggg gcgctcgatc tctctcgcgc gcaggacttc agctgccaga
     1081 tctgcaacgc ggtggatcac gcccatcgcg ccggcgtcct gcaccgcgac ctgcgcccgg
     1141 gcaacatgct catctccgac gccggcatgc tgaaggtcac cgacttcgga acgtcgaggt
     1201 tcctcgagat cgctgcgcac ggcacgacca tcatcggcag cccggcgtac atggccccgg
     1261 agcagtttca cggcaaggcg gtcttcgcca gcgacatcta ttccgtgggc gtcacgatgt
     1321 accagatgct gaccggctcg ctgccgtacg acacaccggc gccggccgac atcgggaagc
     1381 tcgagcgcgg cgagctcgtg tcggcgcccc ggttgaagaa cccgaagatt cccaaggcgc
     1441 tgaacgacat cgtgcttcgg gctcttgcgc cggatccctc tgcgcgctac cagcgcgtcg
     1501 ccgacctgct cgaagatctg cttgcggcgc gggagacggc gccgcgccgc gccgttcggc
     1561 gcggcagcgc ctcggcgaat gccacggtcg ccgagattca ctcgcgcctg aaggcccgcg
     1621 agacgcggcc ggcccggttc tgctggcatt gccgcaagcc gctccacgcg cgaacgaaca
     1681 aatgcccgtt ctgcggggag acacagtagt atcctaggca cgtcggttca ggggattcat
     1741 gcaagtcctc ctgctgtcca tgccggactc gttcgagcac acgccgacgc tcgcagtccg
     1801 gatgccgaac ggcggcctgg cgtcgctggc cggaaacgtg gacacccatc accgcgtcgc
     1861 gatcgcggac ctcatcctcg cccaatccgc cgtccagccg accgtcatca gactgatgaa
     1921 cgagctccgc ccggatctgg tcggtctctc ggtgatgacg ttccagcgcg gcacggccag
     1981 gcgcatcatc tcgctcgtca a
//
LOCUS       DBNGSH010000712         1811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2623436, whole genome shotgun sequence.
ACCESSION   DBNGSH010000712 DBNGSH010000000
VERSION     DBNGSH010000712.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2623436"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="AC16CY_16395"
     CDS             complement(<1..1342)
                     /locus_tag="AC16CY_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3355431.1"
                     /translation="MSDVDRREFLAVVAAAQGAFVLGFFVPPRASAQTTAGTAWSDES
                     TRDEINAWIVIAPDDTVTIRIAQTELGQGVWTSNAMMVCEELQCDWSKVRPQYASANR
                     DAREKAPAWTLKVPGNGATDPAGGGVPTFGNRDRTGVAGIPDSLYRRMRTNAASSVKD
                     GRYYLQLAGAEARERLLLAAAAMWNVPVSELTAKSSVITHASSGRTTTYGRIAQRAAG
                     TPHPHPETIAIKPPDRWTLMGTEQKNLDVPDKVTGETVYGIDVRLPGMKWAAVKTCPV
                     YGGDVKRFDFEPIRNMPGVRSAVRFPIPDPALTRGRVFSGGVAVVADTWHQARTAIDR
                     MPIEWDIPPANAAFNTANMRAALISALDRPGTVRANQGDVDAAFSRAAKIVEATYSTP
                     FLPRARMEPGNATVLVTADRVDVWIGDQSPQETRFSASKITGIPEEQVYLHLCHL"
     gene            complement(1339..1803)
                     /locus_tag="AC16CY_16400"
     CDS             complement(1339..1803)
                     /locus_tag="AC16CY_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3355432.1"
                     /translation="MAFDLIVNGSRHTVDADPQTPLLWVIRDQIGLTGTKFSCGIGQC
                     GACTLHVDGRPIRSCSVPAATAVGKRVTTIEGVSPDGTHPVQLAWKELDAPQCGYCQS
                     GMIMSALALLQERPSPSDADIDARITNVCRCSTYHRVRKAIHLAAGLMKEAR"
BASE COUNT          258 a          629 c          615 g          309 t
ORIGIN      
        1 ccagatggca caagtgcagg tacacctgct cctcggggat cccggtgatc ttcgaggcgc
       61 tgaagcgcgt ttcctgcggg ctctgatcgc cgatccagac gtcaacgcga tccgcggtga
      121 ccagcaccgt cgcgttgccg ggctccatgc gggcgcgcgg caggaacggc gtggagtagg
      181 tggcttcgac gatcttcgcc gcgcgcgaga acgccgcgtc cacatcgccc tgattggcgc
      241 gaacggtccc cggccgatcg agcgcactga tcagcgccgc acgcatgttg gcggtgttga
      301 acgccgcgtt cgcgggcggg atgtcccatt cgatcggcat ccgatcgatc gcggttctcg
      361 cctggtgcca cgtgtcggca acaaccgcga cgccgccgct gaacacgcgc ccgcgcgtca
      421 gcgccgggtc cggaatcggg aagcggacgg cggatcgcac gccgggcatg ttgcggatcg
      481 gctcgaagtc gaagcgcttg acgtcgccgc cgtaaacggg gcacgtcttc acggccgccc
      541 acttcatgcc gggaaggcgc acgtcgatgc cgtagacggt ctcgcccgtg accttgtccg
      601 gtacgtcgag gttcttctgc tcggtgccca tcagggtcca tcgatcgggc ggcttgatgg
      661 cgatcgtctc cggatgcgga tgcggcgtcc cggccgcacg ctgcgcgatc ctgccgtagg
      721 tggtcgtgcg cccgctcgac gcatgcgtga tgacgctgct cttcgccgtc agctcggaca
      781 ccggcacgtt ccacatggcc gcggcggcga gcaacagccg ttcgcgcgcc tcggctccgg
      841 caagctggag atagtaccgg ccgtctttca ccgacgaggc cgcgttggtc cgcatgcgtc
      901 gatagagact gtccgggatt ccggcaacgc cggtccgatc gcgattgccg aacgtcggca
      961 cgcctccgcc cgcgggatcg gtcgcgccgt tgccggggac cttcagcgtc cacgcgggcg
     1021 ccttctcgcg cgcatcgcgg ttcgcggacg cgtattgcgg ccgcaccttg ctccagtcgc
     1081 actgcagctc ctcgcagacc atcatcgcgt tcgaggtcca cacgccctgg cccagctcgg
     1141 tctgcgcgat gcgaatcgtg acggtgtcgt ccggcgcgat cacgatccag gcgttgatct
     1201 cgtctcttgt cgattcgtcc gaccacgccg tgccggccgt cgtctgcgcg ctcgcgcgcg
     1261 gcgggacgaa gaagccgagc acgaacgcgc cctgcgcggc cgcgacgacg gcgagaaact
     1321 cccggcgatc gacgtcgctc atcgcgcctc cttcatcagg cccgcggcga ggtggatcgc
     1381 cttcctgacc cggtggtagg tgctgcagcg gcagacgttg gtgatcctcg cgtcgatgtc
     1441 ggcgtcggac gggctcgggc gctcctggag cagtgcgagc gcgctcatga tcatcccgga
     1501 ctggcagtag ccgcactgcg gcgcgtcgag ctccttccag gcgagctgga ctggatgagt
     1561 gccgtcagga gacacacctt cgatcgtcgt cacccgcttg ccgacggccg tggccgcggg
     1621 gacgctgcag gaccggatcg gccggccgtc cacgtgcagg gtgcaggcgc cgcattgccc
     1681 aatgccgcag ctgaacttgg tgccagtcag cccgatctga tcgcggatca cccacagcaa
     1741 gggcgtctgc gggtccgcat cgaccgtgtg ccggctgccg ttgacgatca gatcgaacgc
     1801 catgcccgcg c
//
LOCUS       DBNGSH010000713         2469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3303086, whole genome shotgun sequence.
ACCESSION   DBNGSH010000713 DBNGSH010000000
VERSION     DBNGSH010000713.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3303086"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="AC16CY_16405"
     CDS             complement(<1..663)
                     /locus_tag="AC16CY_16405"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3355433.1"
                     /translation="MDSTLRQSMFRYYDERAPEYEEAYLLGTGTASIRDPRVFLDEIP
                     MLERVVERFGHDRIIDVACGTAYWLPRYARRCSHITLLDQSERMLDESRKKAARLDIL
                     NRCSIVRADFLEYECPRGSYDSALIGFLFSHLTGDQERLAFAQLQGLLDDDGEFLILD
                     SAWSAQRAQVNTTVGHQKRRLNDGTSFEIYKRYLDDGDIRGWGDKYGVTLRVEHLGMA
                     FLA"
     gene            complement(731..1486)
                     /locus_tag="AC16CY_16410"
     CDS             complement(731..1486)
                     /locus_tag="AC16CY_16410"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIR3355434.1"
                     /translation="MAPLFLSELVGTALLVFGGLSVVILMSGEGSPMVRLVPNEVLRT
                     ILTGFLFGTIGGGIALSRVGRESGAHINPAVTMGFWLMQKLDLRAAAGYIAAQLLGAG
                     LGALPLLAWGSMGRSVAFGATLPGAGYSTGVVLMGEAATTFGLVAALCVFIGFRPLRP
                     FTPYMMPFLYAVMVPLEAAISGTSTNPARTFGPALISGRWDGWWIYWAGPMLGMLIAI
                     LVCSSIARRIEVAKLYHFESDRRRLFRRMAGSR"
     gene            complement(1490..>2469)
                     /locus_tag="AC16CY_16415"
     CDS             complement(1490..>2469)
                     /locus_tag="AC16CY_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HIR3355435.1"
                     /translation="ATRQGGRVLAYGRDPYFAGWPDTLQLDYANPATQEAMRGELLRI
                     AGQCDGVRCDMAMLVLPDVFERTWGRRAPLFWPETTARVRDRVPGFCFMAEVYWGLEW
                     TTQQQGFDYAYDKRLYDRLRDGPARPVREHLLAGLDYQDRLARFMENHDEPRAGVTFE
                     PRRHQAAAIVTYLSPGLRFFHQGQFEGRRKRISPHLVRAPLEPADDAAQRFYERLLAV
                     LRDTTVRDGGWQLLDCVPAWDGNWTWDCCLAWMWTGTAGERRLVVINYAGNQSQCYVR
                     LPALRGSIVRFTDVMGTLVVDRDGHDLAKRGLYLDMPPWGYHVFDVTAS"
BASE COUNT          457 a          833 c          804 g          375 t
ORIGIN      
        1 agccagaaat gccatgccca ggtgttctac gcgaagcgtg acgccatact tgtcgcccca
       61 gccgcggata tccccgtcat cgaggtagcg cttgtagatc tcgaacgacg tgccgtcgtt
      121 caaccgccgc ttctgatgcc cgactgtcgt gttcacctgc gcgcgctggg cgctccacgc
      181 cgaatccagg atcaggaact ccccgtcgtc atcgagcagc ccctggagct gcgcgaacgc
      241 gagccgttcc tggtcgccgg tcagatggct gaacaggaaa ccgatcagcg ccgaatcgta
      301 ggaaccgcgc ggacactcgt actcgaggaa atccgcgcgg acgatcgaac agcgattcag
      361 gatgtccaga cgcgcggcct tcttcctgct ctcgtcgagc atcctctctg attgatcgag
      421 gagggtgatg tgggaacagc gtcgagcgta gcggggcagc cagtaggccg tgccacaggc
      481 gacgtcgatg atccggtcgt ggccgaatcg ttccacgacg cgttccagca tcggtatttc
      541 atcgaggaac acacgcgggt cgcgaatcga ggccgtgcct gtacccagga gatacgcttc
      601 ctcgtactcg ggggcgcgct cgtcgtagta ccggaacatg ctctgtcgaa gagtggaatc
      661 catccgactg cttctgtgcc gacgagggca cttgagtccg ccagcgtttc aggcgggtca
      721 tctgacaagt ctagcgagag ccagccatcc tgcgaaagag ccggcggcga tcgctttcga
      781 agtggtagag cttggccacc tcgatgcgcc tcgcgatgga gctgcagacc aggatggcga
      841 tcaacatgcc gagcatcggc cccgcccagt agatccacca tccgtcccaa cggcccgaga
      901 tcagcgccgg gccgaacgtg cgcgcggggt tcgtgctggt gcccgagatc gccgcttcga
      961 gcggcaccat cacggcatac aggaagggca tcatgtacgg cgtgaacgga cgcaaggggc
     1021 ggaacccgat gaagacgcac aaggcggcga cgagcccgaa cgtcgtcgcg gcctcgccca
     1081 tcagcacgac gcccgtggag tagcccgcgc ccggaagcgt cgcgccgaag gcgacgctgc
     1141 ggcccatcga tccccatgcc agcagcggga gcgcgccgag gccggcgccg agcaactgcg
     1201 cggcgatgta tccagcggcc gctcgcaggt cgagcttctg catcaaccag aagcccatcg
     1261 tcacggcggg gttgatatgg gccccgctct cccgtccaac ccgcgacagc gcaattccgc
     1321 cgccgatcgt gccgaagagg aagccggtca ggatcgtccg cagcacctcg ttcggcacca
     1381 ggcggaccat cgggctgccc tcgccggaca tgaggatgac gaccgacagc ccgccgaaca
     1441 cgagcagcgc cgttcccacg agctcggaaa gaaagagagg cgccatcggt cagctggcgg
     1501 tcacgtcgaa gacgtgatag ccccagggcg gcatgtcgag atacaggccg cgcttcgcga
     1561 ggtcatggcc gtcgcgatcg acgacgagcg tgcccatcac gtccgtgaac ctgacgatgc
     1621 tgccgcgtaa agcgggcagg cggacgtaac actggctctg gttccccgcg tagttgatca
     1681 cgaccagccg ccgctccccc gccgttcccg tccacatcca cgcgagacaa cagtcccacg
     1741 tccagttgcc gtcccacgcg ggcacgcaat cgagcaactg ccaaccgcca tcgcgaaccg
     1801 tcgtgtcgcg gagcaccgcg agcagccgct cgtagaaccg ctgcgcggcg tcatcggccg
     1861 gctcgagcgg cgcgcgcacg aggtgcggcg agatccgctt tctgcgtcct tcgaattgac
     1921 cctgatgaaa gaatcgcagg cccggcgaga gatacgtgac gatggcggcg gcctggtgcc
     1981 tgcgcggctc gaacgtgacg ccggcgcgcg gctcgtcgtg attctccatg aagcgcgcga
     2041 gcctgtcctg atagtcgagg cccgcgagca gatgctctcg cacgggccgt gcagggccgt
     2101 cgcgcaggcg atcgtagagc cgcttgtcgt acgcgtagtc gaagccctgc tgctgcgtgg
     2161 tccattcgag gccccaatag acttcggcca tgaagcagaa gcccgggacc cgatcgcgga
     2221 cgcgcgccgt cgtctccggc cagaagagcg gagcacggcg gccccacgtg cgctcgaaga
     2281 cgtcgggcag gacgagcatc gccatgtcgc agcgtacgcc gtcgcactgt ccggcaatcc
     2341 tgaggagctc gccgcgcatc gcctcctgcg tcgccggatt ggcgtaatcg agctgcaggg
     2401 tgtcgggcca gccggcaaag tacgggtcgc gcccgtaggc cagcacccgg ccgccctggc
     2461 gcgtcgcga
//
LOCUS       DBNGSH010000714         5511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3133946, whole genome shotgun sequence.
ACCESSION   DBNGSH010000714 DBNGSH010000000
VERSION     DBNGSH010000714.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3133946"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC16CY_16420"
     CDS             complement(<1..197)
                     /locus_tag="AC16CY_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3355436.1"
                     /translation="MSLAIGTRLGPYEIHSALGAGGMGEVYRALDTRLARTVAIKVLP
                     EHVRSDPGLQQRFEREARAIS"
     gene            complement(309..2147)
                     /locus_tag="AC16CY_16425"
     CDS             complement(309..2147)
                     /locus_tag="AC16CY_16425"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HIR3355437.1"
                     /translation="MELKTGAYEFGAYRLEPPTRRLLRNGEPVPLTPKAFAVLLALIE
                     RRDRVVDKAELMRIVWPDSFVEEANLSQTIFVLRRTLGDGPDGRPFIETMPRRGYRFA
                     ADVREEIAKPAGYGQHVRWRLRSSWIAATLVIVIALAWFGASGMRLGSDAPVRVESLV
                     VLPFSDLSGDIEDYLGESMTDALITNLGQISGLRVISGTSAMSYKGTSKTVPQIAREL
                     NVDAIVEGTVHRSAGRVSFNLRLIHAATDRTVWAQSYERDVQNALEVHNEIARAIASR
                     TTVTMTERERSRLSEAIPVNPKVYEAYLRGRYFWNQRSEPAMRQAIAHFDEAIRLDSG
                     YAPAYSGLADSYAMYGSHGWSLAGDNAWERAVAAAEKAMEVDELLADGHTSRARIALN
                     YDLDWVGAERGYRRAIELNPGYATARHWYGYYLILSGRFKDGEAEMRRALELDPLSPI
                     INANIGMCFYMARQYDAAIAHWQKALEMHRSYAQLHEYLTMAYVGKEMYPEAAAESQK
                     ILSLSDIAGPEIGILAHVYGRMGRTQEARRMLAEVLSQGESAAYYIALAYVGLGERDN
                     AFKWLDRALQKRAGPFNELNAEPMFDALRADPRFPALLRRMGLSAS"
     gene            2267..3082
                     /locus_tag="AC16CY_16430"
     CDS             2267..3082
                     /locus_tag="AC16CY_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3355438.1"
                     /translation="MRFKGKQAFITGSSRGIGRGIALKMAQEGATIGVHYFRNHEAAA
                     DTLAQVRELGSDGLIVQGDVSKVDDLRRMFAEIRDTFETLDIFVSNARGELATFYEPP
                     LDLTLDQWTTAFDTQARAFFIGAKEAAALMPRGGRILAITFAPGARTGSWQPWIAMGS
                     AKAALESTCRYFAVALASRAITVNAISPGLTEDSVVNTLPEEAVQLARAWHQRWTPMG
                     RLGTPADVGNVAALLCSDEAAWITGQVVCADGGASLMDTVLPLELQLPRMVAV"
     gene            3156..>5511
                     /locus_tag="AC16CY_16435"
     CDS             3156..>5511
                     /locus_tag="AC16CY_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HIR3355439.1"
                     /translation="MTYGQSTEAQAPARPRYRIIDLGTLGGTFSRAYALNNKGQVVGE
                     SLTAANLDVYPFVWTGGTMRAIPGLAGNASASGRLEGINNNGKAVGTWDSGAAGDLGS
                     LFVYDIVSGALSFPLGFGSSFGRNINDGGQILAGRFPPGGGIIADVILTGSGVIDLNA
                     LYGAQFQGEEINAMGHVIGVPGGPTATPDSLIYANGRFTSIFGPIGFIGVAFNDDPSR
                     PAGGFVREQTQFFGIRSRAAARRANGTYQNFGSFNVGADAIVVALNNLGQMVGNERGS
                     GIRLPFIIENGSMRPLSSLLPPNSGWTLLEANDINDRGEIVGMGRINGEDHAYLLTPL
                     TCSSAEDSDGDGDADDDGDGLCDTWETRGVDGDEDGNVDILLNSNPERKDLFVEIDYM
                     VEFVNNPHGHRPRAQALVDVRDAFANAMVVNPDLSFGVTLHLDVDELVPEIETIQFTS
                     NGPGVMDDFNDIKRGNPAGACTGFFGTAAERADPNCEAILKAREMVVRYAVFGHNHTS
                     IGSSGIAESGANDFMVTLGDFDDDMFRANGGSPDLDEARVVVEAGTFMHEFGHTLGLR
                     HGGADHLNCKPNYLSVMNYSFQFRDLVVSRPLDYSFPQLPTLDETQLDETVAFGIGGS
                     HTTVFGAPPDGTPTLGDTSLPIDWNADGAADSAVSADINYIPDGGCSRSEMTFLTGFD
                     DWSNIQYNFRHSPDFADGSFRGTVGNEPERTGPQGLLAAQSFDFDGDGVTNFPDNCPA
                     IPNPTQLDTNGNGVGDACDTSTPADTQPPVTTATLTPEPNAAGWL"
BASE COUNT         1009 a         1810 c         1715 g          977 t
ORIGIN      
        1 ctcgagatgg ctcgagcttc gcgctcgaag cgctgctgaa gcccaggatc cgatcggacg
       61 tgctcgggga gtaccttgat cgcgaccgtg cgggccaggc gcgtgtcgag cgcgcgatac
      121 acctcgccca tgccaccggc tcccagcgct gaatgaatct cgtaaggccc gagccgcgtg
      181 ccgatggcga gagacatgaa cgcggggatc ataccgctga agccgtgcag ggcttcgatc
      241 taccgttcga tccgacgaag ggccgcatcg tgcggctgat gtggcagggc gaatggcatg
      301 aacagcgtct acgaggctga caatcccatg cggcgcagca gcgccgggaa tcgggggtcc
      361 gcccgcaacg cgtcgaacat cggctcggcg ttcagctcgt tgaacggccc cgcgcgcttc
      421 tgaagagcac gatccagcca cttgaacgcg ttgtctcgct cgcccaggcc cacgtacgcg
      481 agcgcgatgt agtacgcggc cgactctccc tgcgacagca cctcggcgag catccgtcgt
      541 gcctcctgtg tccggcccat tcgtccgtag acatgcgcaa ggatgccgat ctccggtcct
      601 gcgatgtcgg acagcgacag gattttctgt gactctgcgg cggcttccgg atacatctcc
      661 ttcccgacat acgccatcgt caagtactcg tgaagctgcg catagctgcg gtgcatctcg
      721 agcgccttct gccagtgcgc gatcgccgca tcgtactgac gggccatgta gaagcacatc
      781 ccgatgttgg cgttgatgat cggtgagagc ggatcgagct ccagcgcgcg gcgcatctcg
      841 gcttcgccgt ctttgaatcg gccgctcagt atcaggtagt acccgtacca gtggcgcgcg
      901 gtcgcgtagc cggggttcaa ctcgatcgcg cgccgataac cgcgctcggc ccccacccaa
      961 tcgaggtcgt agttcaaggc gatgcgcgcc cgcgaggtat gtccgtcagc gagcaactcg
     1021 tcgacttcca tcgccttctc ggcggccgca acggcccgct cccacgcgtt gtctccggcc
     1081 agcgaccagc cgtgcgagcc gtacatcgcg taggagtccg ccaacccgga gtacgcgggc
     1141 gcgtacccgg aatcgaggcg aatcgcctcg tcgaagtgcg cgatggcttg ccgcatcgcc
     1201 ggttccgatc gctggttcca gaaatagcgg cccctcaaat aggcttcgta caccttcggg
     1261 ttcaccggga tcgcttccga caggcgcgac cgctcccgct ccgtcatcgt gaccgtcgtg
     1321 cggctcgcga tggcccgcgc gatttcgttg tgcacctcca gtgcattctg cacgtcgcgc
     1381 tcgtagctct gcgcccagac cgtccgatcg gtcgccgcat gaatcagcct gagattgaag
     1441 ctcacgcgtc ccgccgatcg atgcaccgtg ccttcaacga tggcatcgac gttcagctct
     1501 cgcgcaatct gcgggaccgt cttgctggtg cccttatacg acatagccga cgtgcccgag
     1561 atcacacgca gcccgctgat ctgaccgagg ttcgtgatga gcgcgtcggt catgctctcg
     1621 cccagatagt cctcgatatc gcctgagagg tcgctgaacg gcaggacgac gagcgactcg
     1681 acccgaacgg gagcgtcgct tcccaagcgc atccccgagg ccccgaacca cgcgagggca
     1741 atcacgatca caagcgtcgc ggcaatccac gacgagcgca aacgccagcg gacgtgctgc
     1801 ccatagcccg ccggtttcgc gatttcctcg cgcacatcgg cggcgaagcg atagccacgg
     1861 cgcggcatgg tctcgatgaa gggcctgccg tctggaccgt ctcccaacgt cctgcgcagc
     1921 acgaagatgg tctgtgagag gttggcctcc tccacaaagc tgtccggcca gacaatccgc
     1981 atcagttcgg ctttgtcgac gacccggtcg cgtcgctcga tgagggccag gaggacagcg
     2041 aacgcctttg gcgtcagcgg gaccggctcg ccgtttcgga gcagccggcg ggttggcggc
     2101 tcgagccgat aggcgccgaa ttcgtacgcg ccggttttga gctccatttg aacccgtttt
     2161 gaagcggctt tgaagacttc caggccggcc gccgcctaag ttcaccgcgc tgcccgaccc
     2221 tacggctgcc gattctaccg tgccggccgg gcgttggaga tttgcgatgc gattcaaagg
     2281 caagcaggcc ttcattacgg gcagctcgcg ggggatcgga cgaggcatcg ccctgaagat
     2341 ggcgcaggaa ggtgccacga ttggggttca ctacttccgg aaccacgagg cggccgccga
     2401 caccctggcg caggtgcgcg agctcggctc cgacggtctc atcgttcagg gtgacgtctc
     2461 gaaggttgac gacttgcgaa ggatgttcgc cgaaatccgc gacaccttcg aaacgctcga
     2521 catcttcgtc agcaacgcgc gcggcgagct cgcgacgttc tatgaaccgc cgctggacct
     2581 cacgctcgac cagtggacca cggcattcga cacgcaggcg cgcgccttct tcatcggtgc
     2641 gaaagaagca gcggcgctga tgccgcgcgg cggccggatt ctcgcgatca cattcgcgcc
     2701 gggcgctcga accggaagct ggcagccctg gatcgcgatg ggatcggcga aggcggctct
     2761 cgaaagcacg tgccgctact tcgccgtcgc gctcgcctcg cgcgcgatca ccgtcaacgc
     2821 catcagtcca gggctcacag aagacagcgt tgtgaacacg ctgcccgaag aggccgtaca
     2881 gctcgcgagg gcatggcacc agcgctggac gccaatgggc cggctcggca cgccggccga
     2941 cgtcggcaac gtcgccgcac tcctgtgctc cgacgaggcc gcctggatca cggggcaggt
     3001 cgtctgcgcc gacggcggcg cgtcgctcat ggacaccgta ctcccactcg aactgcagct
     3061 gccgcggatg gtcgcggtct agaggagatc acatgtatcg ctcacttccc cccctccacc
     3121 gttggtgttt cgcgttgctc ctgatccttg gatgtgtgac gtacgggcag agtacagaag
     3181 cgcaggctcc cgcaaggccg cgctatcgga tcatcgatct gggaacactc ggaggcacgt
     3241 tcagcagagc ctatgcactg aacaacaagg gacaagtcgt cggcgagtcg ctgactgccg
     3301 ctaatctcga cgtctacccc ttcgtgtgga ctggcggcac catgcgcgcc attccagggc
     3361 tcgccggcaa cgcaagcgcg tccggcaggt tagaaggaat caacaacaat ggcaaagccg
     3421 ttggcacgtg ggattctggc gcggctggcg acctcggttc actgttcgtg tacgacatcg
     3481 tttcgggcgc gctctcgttt ccgctgggct tcggcagcag cttcggacgg aacatcaacg
     3541 atggcggcca gattctggcg ggccgctttc ctcccggggg tgggattatc gccgacgtca
     3601 ttctgaccgg ctcgggcgtg atcgatctca acgcactcta tggcgctcag ttccagggag
     3661 aagagatcaa cgcgatgggt cacgtgatcg gcgtccctgg gggaccgact gcgacaccgg
     3721 actcattgat ctatgcgaat ggtcgattca cgtccatctt cggtcccatc ggcttcattg
     3781 gcgtggcttt caacgatgat ccgagccgcc cagccggggg attcgtgcgg gagcagacgc
     3841 aattcttcgg cattcgcagc cgcgccgcag cacggcgcgc gaacggaacg tatcagaatt
     3901 ttggctcctt caatgtcggc gccgatgcca tcgtcgttgc tctgaacaac ctcgggcaaa
     3961 tggtcggcaa cgagcgcggc tccggcattc gcctgccatt catcattgag aacggctcga
     4021 tgaggccgct gagctcgctg ctgccgccca actccggctg gacactgctc gaagccaatg
     4081 acatcaacga tcgcggcgag atcgtcggca tgggcagaat caacggggag gaccatgcct
     4141 acctcttgac gccgctcaca tgttcgtcgg ccgaggacag cgacggcgac ggtgacgccg
     4201 acgacgacgg cgatgggctc tgcgatacct gggagacacg cggtgtcgat ggcgacgaag
     4261 acggaaacgt cgatatcctg ctgaactcca acccggaacg caaggatctg ttcgtggaga
     4321 tcgactacat ggtcgagttc gtcaacaatc cccacggcca cagacctcgg gcgcaagcgc
     4381 ttgtggacgt cagggatgca tttgccaatg cgatggtggt gaacccggat ctctcgtttg
     4441 gcgtgacgct gcacctggac gtggacgaac tcgtaccgga gatcgaaact atccaattca
     4501 cgtcgaacgg cccgggcgtg atggacgatt tcaacgacat caagcgcggg aatccggcgg
     4561 gcgcgtgcac ggggttcttc ggcacggctg ccgagcgcgc agatcccaac tgcgaggcga
     4621 ttctgaaggc gcgcgagatg gtggtccgtt atgccgtctt cggccacaac cacaccagca
     4681 ttggttcgag cggcatcgcc gaaagcggcg ccaacgactt catggtcacg ttgggcgact
     4741 tcgatgacga catgtttcgc gccaatggcg gctctccaga cctggacgag gcgcgcgtcg
     4801 tcgtggaagc tggaacgttc atgcacgagt tcgggcacac gctggggttg cggcacggcg
     4861 gcgccgacca tctcaactgc aagccgaact atctcagcgt gatgaactat tccttccagt
     4921 tccgcgatct cgtcgtgagc cgtccgctcg actactcctt tccgcagttg ccgacgctcg
     4981 acgaaacgca gctggacgag acggtagcct tcggaatcgg cggctcgcac acgacggtgt
     5041 tcggcgcgcc gccggacggc acgccgacac tcggcgatac cagcctgccg attgattgga
     5101 acgcggatgg cgcggcagac tctgccgtca gcgccgacat caactacatt cccgacggcg
     5161 gctgttcgcg gagcgagatg accttcctga ccggcttcga cgactggagc aacattcagt
     5221 acaacttccg tcactcgccg gacttcgctg acggctcctt ccgcggcacg gtcggcaacg
     5281 aacccgaacg caccgggccc cagggactgc ttgcggccca atccttcgac ttcgacggcg
     5341 acggggtcac gaacttcccg gacaactgcc cggccatccc caaccccacc caactggaca
     5401 ccaacggcaa cggcgtcggc gatgcgtgcg acacgagcac gccggccgac acacagcccc
     5461 cggtcacgac cgcgacgctg acgcccgagc cgaatgccgc gggatggctg t
//
LOCUS       DBNGSH010000715         2241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2454124, whole genome shotgun sequence.
ACCESSION   DBNGSH010000715 DBNGSH010000000
VERSION     DBNGSH010000715.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2454124"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..446)
                     /locus_tag="AC16CY_16440"
     CDS             complement(144..446)
                     /locus_tag="AC16CY_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR3355440.1"
                     /translation="MPAYWVARARITDPVQYKKYTDLVPAIVARHGGKILARGGRYKI
                     NEGPHDFHRFVVIEFPTLEQGVACFESPEYQQAASFRRAGGGVVENVMVDGGDATQ"
     gene            complement(483..2129)
                     /locus_tag="AC16CY_16445"
     CDS             complement(483..2129)
                     /locus_tag="AC16CY_16445"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3355441.1"
                     /translation="MTRSTSRATLAALALAGAASVIPAQQPASAQGAGATSSAPDVVL
                     TNGKIITVDDRFSIAQAVAIRGDRIVAVGSDAEVGKLATPATRRIDLRGRAVIPGLID
                     NHMHLLRAGATWQSEVRLDGVASRKQLLDMLRARAKTAKPGAWIYTLGGWAVEQLDDK
                     KPFTRAELDAVVPDHPVFLQASYYEAYLNSLALQQMKIDEKSPEWAERDAKGQLTGRI
                     AEAGFRGLVNRIPAPSPDEAQASTAAMIKDLNRFGLTTFGSAGCEANVLPYYQRLAGE
                     SQLNVRVFCITGNEKQIPQMKLFQGNNYFDYVTYGESVYGQLHDPMFLRKSNPTPEQL
                     AEWRRIATEVAKARLPLHVHANLEDTISAFLDQIEAINKEYPIKNLRWVLAHANQLNA
                     THLARMRELGVYAAVHPWAVINGGINQRVFGEAALDMAPLDTIQRSGVIWGFGSDGNR
                     ANQILPFTTLGWAVTGKMVGGTKVLRQPISREDALIAHTRRNAFFVFQENNLGSIQTG
                     RLADLVVLDRDYLTIPADQIKDIKPTMTMVGGRIVYEAAP"
BASE COUNT          338 a          735 c          746 g          422 t
ORIGIN      
        1 ggcagcgcga tggcggccga gcccgccgcg gcgatctcct cggcgaacgg ggccaggacc
       61 gcgaacttca acagggagcc gatagcgcgg cggcgatcga gttcgggcac gacgacctcc
      121 gtgaactgtg aactgcctac tgcctactgc gtggcgtctc ctccgtcgac catcacgttc
      181 tcgaccacgc cgcctccggc gcgcctgaag gacgccgcct gctgatactc cggcgattcg
      241 aagcaggcca ctccctgttc gagcgtgggg aactcgatga ccacgaatcg gtggaagtcg
      301 tgcggccctt cgttgatctt gtagcggccg ccgcgggcga ggatcttgcc gccgtgcctg
      361 gcgacgatcg cgggcacgag gtccgtgtac ttcttgtact gcacgggatc ggtgattctc
      421 gcgcgggcga cccaatacgc aggcatctga aatccttcac cgcgggggct gaagcccccg
      481 ctctacggtg ccgcctcgta cacgatgcgt cctcccacca tcgtcatcgt cggcttgatg
      541 tccttgatct gatcggccgg gatggtgagg tagtcgcgat cgaggaccac gaggtcagcc
      601 agccgcccgg tctggatcga tccgaggttg ttctcctgga agacgaagaa cgcattccgg
      661 cgcgtgtggg cgatgagcgc atcctcgcgg ctgatcggct ggcgcagcac cttcgtgccg
      721 ccaaccatct ttcccgtgac ggcccaccct agcgtggtga acggcaggat ctggttggcg
      781 cggttgccgt cgctcccgaa tccccatatg acgccgctcc gctgaatcgt gtcgagcggc
      841 gccatgtcga gggcggcctc gccgaacacg cgctggttga tgccgccgtt gatcacggcc
      901 caggggtgga cggcggcgta cacgcccaac tcgcgcatcc gcgcgaggtg cgtcgcattg
      961 agctgattgg cgtgcgccag cacccagcgc aggttcttga tcgggtactc cttgttgatc
     1021 gcctcgatct gatcgagaaa cgcgctgatc gtgtcctcga ggttcgcgtg cacgtgcagc
     1081 ggaagacgag ccttggccac ctcggtggcg atgcggcgcc actcggcgag ctgctccggc
     1141 gtcggattcg acttgcgcag gaacatcggg tcgtgcagct ggccgtagac gctctcgcca
     1201 taggtgacgt agtcgaagta gttgttgccc tgaaagagct tcatctgcgg gatctgcttt
     1261 tcgttgcccg tgatgcagaa cacgcgcacg ttgagctgac tctccccggc cagtcgctgg
     1321 tagtacggaa ggacgttcgc ctcgcagccg gcgctgccga acgtcgtcag gccaaagcgg
     1381 ttcaggtcct tgatcatggc cgcggtgctg gcctgtgcct catccggcga cggggcgggg
     1441 atcctgttca ccagcccgcg gaagcctgcc tccgcgatgc gtccggtcag ctgtcctttg
     1501 gcgtcgcgct cggcccactc cggcgacttc tcgtcgatct tcatctgctg gagcgcgagg
     1561 ctgttgagat acgcctcgta gtacgaggct tggaggaaca ccggatggtc gggcaccacg
     1621 gcgtccagtt ccgcgcgcgt gaacggcttc ttgtcgtcga gctgctcgac ggcccatcca
     1681 cccagcgtgt agatccatgc gcccggtttc gcggtcttcg cccgcgcccg gagcatgtcg
     1741 agcagttgct tccgcgacgc gacgccgtcg agccggacct cggactgcca cgtcgcgccc
     1801 gcgcgaagca ggtgcatgtg gttgtcgatc aagcccggga tgacggcgcg tccgcgcaga
     1861 tcgatccgcc gcgtcgccgg ggtcgccagc ttgccgacct cggcgtcgct ccctaccgcg
     1921 acgatgcggt cgccgcgaat ggccacggcc tgggcgatgc tgaagcggtc gtcgacggtg
     1981 atgatctttc cgttcgtcag gacgacgtcg ggcgcagagc ttgtcgcgcc ggcgccttgc
     2041 gcggaggcag gctgctgcgc gggaatgacc gatgccgcgc ccgcaagcgc cagcgcggcc
     2101 agggttgcgc gggaagtgga ccgtgtcatt gtggcctccc gcgtgggatc tgaccccaat
     2161 tcataccgtt tgacaacccc cgactgcggg cgataatcgg cgtggtcagg agatcaccat
     2221 gaaaacgctc cggttcgtcg t
//
LOCUS       DBNGSH010000716         2505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1775347, whole genome shotgun sequence.
ACCESSION   DBNGSH010000716 DBNGSH010000000
VERSION     DBNGSH010000716.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1775347"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="AC16CY_16450"
     CDS             complement(<1..1747)
                     /locus_tag="AC16CY_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HIR3355442.1"
                     /translation="MTALDWVMVLAYFGLLLGLTWWSVRRNRNTADDYFLAGRHLGWM
                     VVGASIFASNIGSEHLVGLAGAGATSGVALAHYELHAWCLLVLGWVMVPFYLRSAVFT
                     TPEFLERRFSSTARLVLSIISLVAYVLTKMAVGIFAGGVVFGVLLPELRIDIGGTIFN
                     SFWIGSVAVVALTGLYTVAGGMRAVAYTEAVQTAVLVLGSLLLTVFGLYQLGGWSELR
                     RALEPELFNLWRPLVPAGVEGTWAPVREAGRMAWYFNQDYPWIGMLLCAPIIGLWYWC
                     TDQYIVQRTLAASSERDARRGTIFAAFLKLLPVFIFIIPGMIALALARTGRAPGLETL
                     VDGSGTVVPAAAQASFPLLVQSVMPAGLRGIVVAGLLAALMSSLAGAFNASSTLFTLD
                     LYQKLRPRASQAQLVWVGRVATTIMVLIALAWIPVIQGARGLYDYLQGVQAYLAPPIA
                     AVFFLGVFMRRLTAAGCLAALGAGFALGLVRLAVDTPVTLGLDGYASGYTSGSLLWIL
                     NNIYFQYYSLLIFLVSVVVLIGVSLVTAPPSPAQLAGLTYATITRQQQRESRASWNRW
                     DVINSVIVLLLIAAAY"
     gene            1878..2462
                     /locus_tag="AC16CY_16455"
     CDS             1878..2462
                     /locus_tag="AC16CY_16455"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR3355443.1"
                     /translation="MQKPPVVLLAVVLLAAGAFALASPIAAQQVRATGGAVTSFYDLD
                     TMTLDGKPGNLAQYKGKVSLVVNVASKCGFTPQYEGLEKLQREMKGKAFNVLGFPSND
                     FGGQEPGTAQEIATFCKLTYDVTFPMFEKVVTRKGAGQSSVYTFLGSSGSLPAWNFAK
                     YVVDKQGKVTAFFESKVTPEDPALRAAIAKALGS"
BASE COUNT          479 a          846 c          846 g          334 t
ORIGIN      
        1 cgtacgcggc ggcgatcagc aggagcacga tgacggagtt gatcacgtcc cagcgattcc
       61 agcttgcgcg cgactcgcgt tgctgctggc gcgtgatggt tgcgtaggtc agcccggcga
      121 gctgcgccgg agatggcggc gcggtcacca ggctgacacc aatcagcacg acgaccgaga
      181 ccaggaagat cagcaggctg tagtactgaa agtagatgtt gttcaggatc cacagcagcg
      241 atcccgaggt gtacccgctc gcgtagccgt ccagccccag cgtgaccggc gtgtccacgg
      301 ccaaccggac cagcccgagc gcgaagcccg ctccgagggc ggccagacaa ccagcggcgg
      361 tcagccgccg catgaacacg ccgaggaaga agacggccgc aatgggcggc gcgaggtagg
      421 cctgcacgcc ctgcaggtag tcgtagagcc cgcgcgcgcc ctggatgacg ggaatccacg
      481 ccagcgcgat gagcaccatg atcgtcgtcg cgacgcggcc gacccacacg agctgcgcct
      541 gcgacgcgcg cggcctgagc ttctgataca ggtcgagcgt gaagagcgtg gacgaggcgt
      601 tgaaggcgcc ggcgagcgag ctcatgagcg cggcgagcag accggcgacg acgatcccgc
      661 gcaggcccgc cggcatcacg gactgcacga gcagcgggaa agacgcctgc gcggcggcgg
      721 ggaccacggt gcccgagccg tccacgagcg tctcgagtcc tggcgctctt cccgtcctgg
      781 cgagcgcgag cgcgatcatg ccggggatga tgaagatgaa caccggcagc agcttgagaa
      841 acgcggcgaa gatcgtcccg cggcgcgcgt cgcgctcgct cgacgcggcg agcgtgcgct
      901 gcacgatgta ctgatcggtg caccaatacc acagtccgat gatcggcgcg cacagcagca
      961 tgccgatcca gggatagtcc tggttgaaat accacgccat ccggcccgct tcgcgcacgg
     1021 gcgcccaggt gccttcgaca cccgccggaa cgagcggccg ccagaggttg aagagctccg
     1081 gctcgagcgc gcgtcgaagc tcgctccagc cgccaagctg atagagcccg aacacggtca
     1141 gcagcagcga tcccagcacc agcaccgccg tctgaaccgc ctcggtgtag gccaccgcgc
     1201 gcatgcctcc ggccaccgtg tagaggcccg tcagcgccac gacggcgacc gatccgatcc
     1261 agaagctgtt gaagatcgtg ccgccgatgt cgatgcgcag ctccggcagg agcacgccga
     1321 acaccacgcc gcccgcgaag atgccgacgg ccatcttcgt cagcacgtac gccacgagcg
     1381 agatgatcga cagcacgagc cgagcggtcg aggagaaccg ccgctcgagg aactccggcg
     1441 tcgtgaagac cgccgaccgc aggtagaacg ggaccatgac ccagccgagc acgaggaggc
     1501 accatgcgtg gagctcgtag tgcgcgaggg cgacgccgct cgtggcgccg gccccggcca
     1561 ggccgaccag gtgctccgac ccgatgttgg acgcgaagat cgacgcgccg acgaccatcc
     1621 acccgagatg gcgccctgcc aggaagtaat cgtccgccgt gttccgattc cggcggacgg
     1681 accaccaggt caggccgagg agcaggccga aatacgccag gaccatgacc cagtcgagcg
     1741 cggtcatgtc agcctgctcc ctgcggcggc gtggtcatcg tgtccgcgac atcatacggg
     1801 atggcccacc ccaccgcgcc cacaacgcgc ggcgggggcc ccgggggcgc cgagctattt
     1861 cgcgtaacat aggcggcatg cagaaaccac ccgtcgtact tctggctgtc gtgctgctcg
     1921 cggccggcgc ctttgctctc gcgtcgccca tcgcggccca gcaggtgcgc gccactggag
     1981 gggccgtgac ctcgttctac gatctcgaca cgatgacgct cgacggcaag ccgggcaacc
     2041 tggcgcagta caaggggaag gtgagcctgg tcgtcaacgt ggccagcaag tgcgggttca
     2101 cgccgcagta cgaaggcctc gagaagctgc agcgcgagat gaagggcaag gccttcaacg
     2161 tcctcgggtt cccgagcaac gacttcggcg ggcaggagcc cggcaccgcg caggagatcg
     2221 ccaccttctg caagctgacc tacgacgtga cgttcccgat gttcgagaag gtcgtcacca
     2281 ggaagggcgc ggggcaatcg tccgtctaca ccttcctcgg gtcgtcgggc agtctgccgg
     2341 cctggaactt cgccaagtac gtcgtcgaca agcagggcaa ggtgaccgcc ttcttcgaga
     2401 gcaaggtgac gcccgaggat ccggcgctgc gcgcggcgat cgcgaaggca ctcggcagct
     2461 agagcccacc tcgatccttt tgttcgttcg cgacgtctaa gggtt
//
LOCUS       DBNGSH010000717         6358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2624168, whole genome shotgun sequence.
ACCESSION   DBNGSH010000717 DBNGSH010000000
VERSION     DBNGSH010000717.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2624168"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="AC16CY_16460"
     tRNA            complement(1..76)
                     /locus_tag="AC16CY_16460"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Phe,seq:gaa)
     gene            118..504
                     /locus_tag="AC16CY_16465"
     CDS             118..504
                     /locus_tag="AC16CY_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR3355444.1"
                     /translation="MSDSVRLDVWLDIACLFKTRSEAKRACEGGKVDVNGDSAKPHRA
                     VRESDRIRITRGHGRYQQIVVRLILDQHVKKSEAKALYDDVTPKPTPEEIETRRLERI
                     YRAAARASSNPDRRRRREIRRMKEGG"
     gene            508..1872
                     /locus_tag="AC16CY_16470"
     CDS             508..1872
                     /locus_tag="AC16CY_16470"
                     /inference="COORDINATES: protein motif:HMM:NF025473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HIR3355445.1"
                     /translation="MYLSLFQIASLAIVGWIPLIFFPTSRLSRRLADSAFFPLYIAVL
                     YAFGVGALLMESGPGFIADFGTAEGVARLLARQEIAWVAWLHILAFDQVIGLLIYREN
                     MRRRYVPLPVQSALLFLTLMFGPLGYLAFAVIRVARLGPRAFGEDDDSAREPARRAAS
                     DTPPTIRQIVQTFREERGVTMTALAGIALGGIAFILAAVRGPLIPPEGDLMKPAAFDV
                     AVGLFILSLIPWLSVSGFSEAGRRRWRGWMIGLLIYGFGVETIQQFRGIDPRFSQAEP
                     GTSIFGGIFFATALGIMTLSVALAARAFEVRTEGRGGLLVVAARWASASMLMGFLAGF
                     WLSANQGRFVGESGNLLPLHAAGFHAVQAVPLVALMLAWSAMPVGIARRWVHVAGAAW
                     ATACLVIWVQTALGRAVTDLASVAGAAAAALLGVWMLAALRALVAWRTAPAAGAFAGT
                     ASKL"
     gene            1937..2977
                     /locus_tag="AC16CY_16475"
     CDS             1937..2977
                     /locus_tag="AC16CY_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355446.1"
                     /translation="MLQNSRVAALLAVSVLTLVSATSAQWLTQPTAGIPRLPDGKPNL
                     SAAPPRSVDGKPDLSGLWHAGPKWDTDLKETDVQPWAQEQARQRLANPASLGWSVLCL
                     PPGPMVTFSGPLKIIQTPQLIAVLYEVPNNFRQIFLDGRRLPSDPNPTWQGYSVGRWE
                     GETLVVESNGFNDKSWVGRPGFPHTEALRVTERYSRRDVGHIDVQITFDDPKTFVRPW
                     TTTTELLYDPDTEMLEFVCNENEKSVQHFVQPPQGAPAINVDPAMLAKFPGVYQMMTP
                     RGMANATVTLDGGQLMIDVPGFGSGRMVPQSATMFQFRGAVLEFVSNEKGEVTHLIAH
                     VVEGDFKGPRIK"
     gene            2987..4000
                     /locus_tag="AC16CY_16480"
     CDS             2987..4000
                     /locus_tag="AC16CY_16480"
                     /inference="COORDINATES: protein motif:HMM:NF052219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3355447.1"
                     /translation="MPRNQEVIRQWKILHALESSRHGATIDGLAGDLDVTTRTIRRDL
                     AALQEAGFALYDEHDDEGRVRWRVDGQILKGLESGFTLSELCALYLSRNLLEAVAGTP
                     FQRDLTNAFERLAKMLSPRMRQFLDRLPSVLVAKPGPRARGAGASPDIVARLLEASLH
                     FRVTLMRYHSVSSSRVKDYLVHPYRLAFAHGGLYLLAYVPDYKAVRTFAVDRIKSVSL
                     EKQTFTTTQAVTDDVFANSLGVNTGPAEPVEIEFDSRVAPYVRARVWHPSQQLQERPD
                     GGLSVTMNVCHDWGLRSWVLSWGPFARVVSPARLSREVQSDLGSAAARYQPQSDSPVD
                     RRP"
     gene            4023..>6358
                     /locus_tag="AC16CY_16485"
     CDS             4023..>6358
                     /locus_tag="AC16CY_16485"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family membrane protein"
                     /protein_id="HIR3355448.1"
                     /translation="MSRPVRFTLLALAALVFLAGPSLLRFYTDWLWFGEVGYQHVFLT
                     MLRMQGTLFTIAFAASALWLSFNVSAAIGSMGNLRPVFVGRDGVEMSLPVGRQLRPIA
                     VGLALLLSLIVGMFAAGRWEQWLLWRSGGPFGEQDPLLGLDIGFYVFSLPFLQLVRRI
                     AQALVILAGLASAGIYLLSGSLTSGFPARLSMTPAARRHLALLGALFMLLLAWGAWLQ
                     RAEHLIDASGTVPGASYADVYGRMPMAMFLVVVSIVGAALAAFHAFGTRNWPIPAAVG
                     LYVLTSIGGEVYSSVLQKFVVTPNEQARETPFITHNITATRKAFGLDSIEERPLSGEA
                     LLTRNDIARNAATLQNVRLWDHQPLLETFGQLQEIRTYYEFTSVDNDRYRIDGALRQV
                     MLSARELNSTSLSNRTWVNERLMFTHGYGVALGPVNQVTSEGLPVLFVRDLPPQSTID
                     LKIDQPSIYFGELSSDHVIVGAKVDEFHYPRGDGDAMTRYDGAGGVALGTIWRKILFA
                     LRFRSYQILLSNDIGPESRILYNRRIRDRVMTLAPFVRFLDRDPYLVIADGRLYWLYD
                     AYTASAFYPYSSPSPGGQVNYIRNSIKFVIDAYDGTITAYLADPNDPIAATFARVFPN
                     LFKPLDEMPASIRAHVRYPEDIFELQAQVFATYHMTEAPVFYNREDQWEIPRLGDGSD
                     AVAMQPYYTIMRLPGEAEAEFIQMLPFTPRQRQNLAAWLVARSDGEHYGKMRVFEFPK
                     QKQVFGPSQVVGRIGQDQVISPQITLWNQQGSQVIWGTL"
BASE COUNT         1032 a         2110 c         2053 g         1163 t
ORIGIN      
        1 tggtggccag ggccggaatc gaaccagcga caccgtgatt ttcagtcacg tgctctacca
       61 actgagctac ctggccctcg tacgcgtcgt cgggcaagac ttctgactat aacagatgtg
      121 agcgacagcg ttcgcctgga tgtctggctc gacatcgcct gtctgttcaa gaccaggtcc
      181 gaagccaagc gcgcgtgcga gggtggcaag gtcgacgtga acggcgacag cgcgaagccg
      241 catcgcgccg ttcgcgaaag cgatcgcatc cgcatcacgc gcgggcacgg ccgctaccag
      301 cagatcgtcg ttcggctgat cctcgaccag cacgtcaaga agtcagaggc caaagcgctc
      361 tacgacgatg tgacgccgaa gccaacgccc gaggagatcg aaacacgccg tctcgagcgc
      421 atctatcgcg ccgccgcgcg cgcgtcgtcc aaccccgatc gccgccgccg gcgtgaaatc
      481 cgccggatga aggagggtgg atagatcatg tacttgagtc tcttccagat cgcgtcgctc
      541 gccatcgtcg ggtggatccc gctgatcttt tttcccacgt cgcgcctgtc gcggcgtctg
      601 gccgacagcg cgttcttccc tctctacatc gccgtgctct atgccttcgg tgtcggcgcg
      661 ctgctgatgg agtccggacc ggggttcatc gccgatttcg ggacggccga gggcgtggcc
      721 cggctgctgg cccgccagga gattgcctgg gtcgcgtggc tgcacatcct cgccttcgac
      781 caggtgatcg ggctgctcat ctaccgcgag aacatgcgcc ggcgctacgt gccgttgccg
      841 gtgcagtcgg cgctgctctt cctcacgttg atgttcggtc cgttggggta cctcgcgttc
      901 gccgtgatcc gcgtggcgcg gctcggcccg cgtgcgtttg gtgaagacga tgacagcgct
      961 cgcgagccgg cccggcgggc agcctccgat acgcctccga cgatccggca gatcgtccag
     1021 accttccgcg aggagcgggg cgtgacaatg acggcgctcg cgggcatcgc cctgggcgga
     1081 atcgctttca tcctggccgc ggtgcgcggc ccattgattc cgcccgaagg cgacctcatg
     1141 aagccggccg cattcgatgt tgcggtgggg ctgtttattc tgtcgctcat cccctggctg
     1201 tcggtgtctg gtttcagcga agcggggcgg cggcgctggc gcgggtggat gatcggcttg
     1261 ctcatctacg ggttcggcgt cgaaacgatc cagcagttcc gcggcatcga tccgcgcttc
     1321 tcgcaggccg agccgggcac ctcgatcttc ggcgggatct tcttcgccac ggcgctgggg
     1381 atcatgacgc tttcggtggc actcgcggca cgcgcgttcg aggtccgtac cgagggccgg
     1441 ggagggctac tcgtcgtggc ggcacgctgg gccagtgcat cgatgctcat gggattcctc
     1501 gccggcttct ggctgagcgc caatcaggga cgcttcgtcg gcgagtcggg gaaccttctg
     1561 ccgcttcacg cagccggctt tcatgccgtg caggccgtgc cgctcgtggc gctgatgctc
     1621 gcctggtcgg ccatgcccgt cgggatcgcc cggcgctggg tgcacgttgc cggcgccgcg
     1681 tgggccaccg cctgcctcgt catctgggtg cagacggcgc tgggacgcgc cgtgaccgat
     1741 ctcgcctccg ttgcgggagc cgccgccgcc gcgctgctgg gtgtctggat gctggcggcg
     1801 ctgcgcgccc tcgtcgcgtg gcgcaccgca cccgccgctg gcgcgtttgc cggtacggcc
     1861 tccaaactct aagaaggcac aaagttctgg ccgacgaggc gcctataatc gacggtcggg
     1921 tctgcgaggc aatcccatgc tccagaattc gcgcgtcgcg gcactcctcg ccgtttccgt
     1981 tctgactctc gtctcggcca cctccgctca atggctgacg caaccgaccg cagggattcc
     2041 gcgcctccct gacggtaagc cgaacctgtc tgcggcccct ccgcgatcgg tcgacggcaa
     2101 acctgatctc tcggggctgt ggcacgccgg acccaagtgg gacaccgatc tcaaggagac
     2161 cgacgttcag ccctgggccc aggaacaggc acgacaacga ctggcgaatc ccgcgtcgct
     2221 cggatggagt gtcctgtgcc tgccgccggg cccgatggtg accttcagcg gaccgctcaa
     2281 gattattcag acgccgcaac tcatcgccgt cctctacgag gtgccgaaca acttccgtca
     2341 gatcttcctc gacggccggc gccttccgag cgaccccaac ccgacgtggc agggctactc
     2401 ggtcggacgc tgggagggcg agacgcttgt cgtcgagagc aacggcttca acgacaagtc
     2461 gtgggtcggg cgtccgggtt ttccacacac ggaagcgctg cgtgtgactg agcgctacag
     2521 cagacgtgac gtcggtcata tcgacgtgca gatcaccttc gacgatccaa agacgttcgt
     2581 gcgaccgtgg acgacgacga ccgagctcct ctacgaccct gacacggaaa tgctggaatt
     2641 cgtctgcaac gagaacgaga agagcgttca gcatttcgtc cagccaccac agggtgcgcc
     2701 agcgatcaac gtcgacccgg ccatgttggc gaagttcccc ggcgtctacc agatgatgac
     2761 gccgcgcggt atggcaaacg cgaccgtcac gctggacggc gggcagctga tgattgacgt
     2821 cccggggttc ggcagcggcc gcatggttcc gcaatcggcg acgatgtttc aattccgggg
     2881 cgccgtgctg gagtttgtgt cgaacgagaa gggcgaggtg acgcacctca tcgcgcacgt
     2941 cgtggagggc gatttcaagg gaccgcggat caaataggat cgacccgtgc cgcggaacca
     3001 ggaagtcatc aggcagtgga agatccttca tgcgctcgag tcgtcgcgtc acggcgcgac
     3061 gatcgacggc ctcgccggcg atctcgacgt caccacacgc accatccggc gggacctcgc
     3121 ggcgctccag gaggcgggct tcgccttgta cgacgagcac gacgacgagg ggcgcgtcag
     3181 gtggcgcgtg gacggacaga tcctcaaggg gctcgagagc ggcttcacgc tgtcggagtt
     3241 gtgcgcgctc tacttgagcc gaaacttgct cgaagccgtc gccggaacgc cgttccaacg
     3301 ggacctgacc aacgcgttcg agcggctggc gaagatgctg tcgccacgga tgcggcagtt
     3361 cctggaccgc ctgccttccg tgctcgtggc caaacctggc ccgcgtgcgc gaggcgcggg
     3421 cgcctccccc gatatcgtgg cgcggctcct cgaggcgtcg cttcacttcc gcgtgaccct
     3481 catgcgttac cactccgtgt cgagcagccg cgtgaaggac tacctcgtcc acccttaccg
     3541 gctggcgttc gcccacggcg ggctgtacct gctcgcctac gtgcccgact acaaggccgt
     3601 gcgtaccttc gcggtggacc ggatcaagtc cgtgtcgctc gagaagcaga ccttcacgac
     3661 cacacaggcg gtgaccgacg acgtgttcgc caactcgctc ggagtaaaca ccgggccagc
     3721 cgaaccggta gaaatagaat tcgattcacg cgtcgccccg tacgtgcgcg cacgtgtctg
     3781 gcatccgtca cagcagctgc aggagcggcc tgacggcggc ctttccgtca cgatgaacgt
     3841 gtgccacgat tgggggcttc gaagctgggt cttgagctgg ggaccgtttg ccagggtcgt
     3901 gtcaccggca aggctgtcgc gagaggtcca atccgacctc ggatcggcgg cggcccgcta
     3961 ccagccgcaa tctgattcgc ctgtagaccg tagaccgtaa accggagacg ccccgagatc
     4021 caatgagccg acctgtccgc ttcaccttgc tcgccctcgc cgcgctggtg ttccttgccg
     4081 gcccgtccct cctgcgcttc tacaccgact ggctctggtt cggcgaagtc ggctaccagc
     4141 acgtcttcct gacgatgctg cggatgcagg gaacgctgtt cacgatcgcg ttcgccgcgt
     4201 ccgcgctctg gctgtctttc aacgtgagcg ccgcgatcgg gtcgatgggc aacctgcgcc
     4261 cggtgttcgt gggccgcgac ggggtcgaga tgtcgttgcc cgtcggccgg cagctcaggc
     4321 cgatcgccgt gggactcgcc ctcctgctgt ccctgatcgt cgggatgttc gcggcggggc
     4381 gctgggagca atggctgctc tggcgatccg gcggcccgtt tggcgaacag gatcccctgc
     4441 tcggcctcga catcggcttt tacgtcttct cgctcccgtt cctgcagctc gtccgccgga
     4501 tcgcgcaggc gctcgtcatt ctcgcgggtc tggccagcgc gggcatctac ctgctgtcgg
     4561 gaagcctgac gtcgggcttc ccggcgaggc tgtcgatgac gccggccgcg cgccggcacc
     4621 tggcgctgct cggcgcgctc ttcatgctgc tgctcgcctg gggcgcgtgg ctgcagaggg
     4681 ccgagcatct catcgacgct tccgggacgg ttccaggcgc cagttatgcg gacgtgtacg
     4741 ggcgcatgcc gatggcgatg ttcctcgtgg tggtgtcgat tgtcggcgcg gcgctggcag
     4801 cctttcacgc cttcggcacg cggaactggc cgattccggc cgccgtcggt ctgtacgtgc
     4861 tgacctcaat cggcggcgaa gtgtacagct cggtactgca gaagttcgtc gtgacaccca
     4921 acgagcaggc gcgcgaaacg ccgttcatca cgcacaacat cacggcaacg cgaaaggcgt
     4981 tcggcctcga ctcgatcgaa gagcggccgc tctcgggaga agcgctgctg accaggaacg
     5041 acatcgcccg caacgccgcg accctgcaga acgtcaggct ctgggatcac cagccgctgc
     5101 tcgagacgtt cgggcaactc caggagatcc gcacctacta cgagttcaca tcggtcgata
     5161 acgaccgcta ccggatcgac ggcgccctgc ggcaggtgat gctgtccgcc cgcgagctga
     5221 actcgacgag cctgtcgaac cgcacgtggg tgaacgaacg gttgatgttc acgcacggct
     5281 acggggtggc gctcgggccg gtcaatcagg tgaccagcga ggggctgccg gtgctcttcg
     5341 tgcgcgacct tcctccgcag tcgacgatcg acctgaagat cgaccagccg agcatctact
     5401 tcggcgagct ctcgagcgac cacgtcatcg tcggcgcgaa ggtcgacgag ttccactatc
     5461 cccgcggcga cggcgacgcg atgactcgct acgacggcgc gggcggcgtg gcgctgggaa
     5521 cgatctggcg caagatcctc ttcgcgcttc ggttcaggtc gtaccagatt cttctcagca
     5581 acgacatcgg accggagagc cggatcctct acaaccgcag gatcagggat cgcgtgatga
     5641 ccctggcgcc tttcgtgcga ttcctggacc gcgaccctta cctggtgatc gcggacggcc
     5701 gtctgtactg gctgtacgac gcctacacgg cgagcgcttt ctacccgtat tcgagtccga
     5761 gtcctggcgg tcaggtgaac tacatccgca actcgatcaa gttcgtcatc gacgcctacg
     5821 acggcacgat cacggcctat ctcgccgacc cgaacgatcc gattgccgcg acgttcgccc
     5881 gcgtgtttcc gaacctgttc aagccgctcg acgagatgcc tgcctcgata cgcgcgcacg
     5941 tgcgctaccc ggaagacatc ttcgagctgc aggcccaggt attcgcgacg tatcacatga
     6001 cagaagcccc cgtcttctac aaccgagagg atcaatggga gattccacgg ttgggagacg
     6061 gcagcgacgc cgtggcgatg cagccctact acacgatcat gcggctcccc ggtgaagccg
     6121 aggccgaatt catccagatg ctcccgttca cgccgcggca gcgccagaat ctggctgcgt
     6181 ggctcgtggc gcgcagcgac ggcgagcact acggcaagat gcgcgtgttc gagttcccga
     6241 aacagaagca ggtgttcggt ccaagccagg tcgtgggccg tatcggccag gatcaggtca
     6301 tctcgccgca gatcaccctc tggaatcaac agggctcgca ggtcatctgg ggcacgct
//
LOCUS       DBNGSH010000718         2470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3303916, whole genome shotgun sequence.
ACCESSION   DBNGSH010000718 DBNGSH010000000
VERSION     DBNGSH010000718.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3303916"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="AC16CY_16490"
     CDS             complement(<1..1237)
                     /locus_tag="AC16CY_16490"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355449.1"
                     /translation="MSAVRTAQAIVARDLSRAVRQKGRLFGGLARSFMWLLLVGAGYG
                     AIARVDGSSTYQAFVFPGIVVMASLFGAMLTAISTVYDREFGMLRLMLACPSGVPAVL
                     AGRAVAAAAVGFLHGAIVLATAPFFVQVTLSQAGLALGVLALTSASSSVLGLLVAAPL
                     RSVENFAAVVNVVLFPLLFLSGALYPTANMPPWLHAVAVLNPVTYAVDLMRESLGQPA
                     EFPVALSIGALGGTIALAFTAAALLFDPEQRLVLPGTRRARHASLWLVMAASIAALGA
                     QRPDAPDLILHNARIYTADVKRSIAEAVAIKGDRILRVGGGTDILALRTPSTRVVDVR
                     GATVLPGLHDAHGHVVEFGASMQNLDLRGTRSFEEIVGRVRRRVADARPGEWILGRGW
                     DQNDWAEHDWPTHEILSAAS"
     gene            complement(1234..2250)
                     /locus_tag="AC16CY_16495"
     CDS             complement(1234..2250)
                     /locus_tag="AC16CY_16495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355450.1"
                     /translation="MSVPVPPKPREGGPVSPKLREGGPAIETTGLGRDYLDVRALDTL
                     SIAVDRGSTVGVLGPNGAGKTTTLLMLATLLAPTRGKALVFGHDITRERAAIRRRLGL
                     VFQEPSIDGLLTVEENLRFAAGLMGMGGAGRQQAVSEALARTGLVEQASRPARRLSGG
                     FRRLTDIARATVHGPDLLLLDEPTSGLDPEHRDRVWALLEAERRTRGVTVVFSTHYLA
                     EAESCDRVFMLGSGRLVADDTPASLRATVGEEVVEVEGPDAERAARSLESAADVRMTV
                     RTERGYRIGVSGARDALSTIVGAAPRLARVGVRAATLEDVYFARTQSASSPKPGVRSP
                     EPVS"
     gene            complement(2247..>2470)
                     /locus_tag="AC16CY_16500"
     CDS             complement(2247..>2470)
                     /locus_tag="AC16CY_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HIR3355451.1"
                     /translation="DEWMQEPCRSCPRKSIDFGGCRCQAFALTGDATATDPVCTLSPS
                     RAVIDAAIRDAADPPDYRYRYITLEPART"
BASE COUNT          404 a          875 c          849 g          342 t
ORIGIN      
        1 gcgaggcggc gctcaggatc tcgtgcgtgg gccagtcgtg ttccgcccag tcgttctggt
       61 cccacccgcg cccgagaatc cactcccccg gcctcgcgtc cgccacccgt cgtcgaacgc
      121 ggccgacgat ctcctcgaag ctgcgcgtgc cgcgcaggtc gaggttctgc atcgaggccc
      181 cgaactcgac gacgtgcccg tgtgcatcgt gcaatccagg caggacggtt gccccgcgca
      241 cgtctacgac gcgagttgaa ggcgtcctga gcgcgaggat gtcggtgccg ccgccaactc
      301 ggagaatgcg atcgcccttg atcgcgaccg cttcggcgat ggagcgcttc acgtccgcgg
      361 tgtagatcct ggcgttgtgc aggatcaggt ccggcgcatc gggtcgctgg gcgccgaggg
      421 cggcgatcga tgccgccatg accagccaaa gcgacgcgtg gcgcgcccgt cgcgtccccg
      481 gcagtaccag gcgctgctcg ggatcgaaga gaagggccgc tgccgtaaac gcgagtgcga
      541 tcgttccacc gagcgcgcca atcgacaacg ccaccgggaa ttcagcgggt tgtccgagcg
      601 attccctcat cagatcgacc gcataggtca ccgggttcaa caccgcaacc gcgtgcagcc
      661 agggcggcat gttcgccgtc ggatagagcg cgccgctcag aaacagcagc gggaagagca
      721 cgacgttgac gacggcggca aagttctcga cggaccgcag gggcgcggcc accaacaggc
      781 caaggacgct gctcgacgcc gacgtcagcg cgagaacgcc gagcgcgagt ccggcctgcg
      841 acagcgtcac ctgcacgaag aagggcgcgg tggcgagcac gatcgcgccg tgcaggaagc
      901 ctaccgcggc cgcggccacc gcccggcccg ccagcaccgc cggcaccccc gacgggcagg
      961 caagcatcag gcgcagcatg ccgaactcgc ggtcgtacac cgtggagatc gccgtgagca
     1021 tggcgccaaa gagcgacgcc atcacgacga tgccagggaa gacgaacgcc tgatacgtcg
     1081 aactaccgtc cacgcgcgcg atggcgccgt agccggcacc cacgaggagg agccacatga
     1141 acgagcgcgc gaggcccccg aacagcctgc ccttctgccg aacggcgcgc gaaaggtcgc
     1201 gggcgacgat cgcctgtgcc gttcgaacag cgctcatgac acgggctccg ggcttcggac
     1261 tccgggcttc ggactcgagg cgctctgcgt tcgtgcgaaa tacacgtcct cgagcgttgc
     1321 ggcgcggacg cctacccgcg ccagacgggg tgcggcgccg acgatcgtcg aaagcgcgtc
     1381 gcgcgcgccg ctgacaccga tccggtagcc gcgttcggtg cggaccgtca tgcggacgtc
     1441 ggccgcgctc tcgagggagc gggctgctcg ctccgcgtcg ggaccttcga cttcgacgac
     1501 ttcctcgcca accgtcgccc tgagcgaggc cggcgtgtcg tcagccacga gccggccgct
     1561 gcccagcatg aacacgcgat cgcacgactc ggcctcggcg agatagtgcg tggagaacac
     1621 caccgtgacg ccgcgcgtgc ggcgctcggc ttcgagcagc gcccacacgc gatcccggtg
     1681 ctcgggatcg agtccggatg tgggctcgtc gagcaggagc agatccggcc cgtgcaccgt
     1741 agccctcgcg atatcggtga ggcgccggaa gccgcccgac agccgcctcg ccgggcgcga
     1801 tgcctgttcg acgaggcccg tgcgggccag cgcctccgac acggcttgct gccgcccggc
     1861 gcctcccatg cccatcaggc cggccgcgaa gcggaggttt tcttccaccg tcagcagtcc
     1921 gtcgatgctc ggctcctgga acacaagacc gagacgccgg cggatggccg cgcgctcgcg
     1981 cgtgatgtcg tgtccgaaga cgagcgcctt cccgcgcgtg ggagcgagga gcgtggccag
     2041 catcagcagc gtggtcgtct tgccggcgcc gttcgggccg agcacgccga cggtcgagcc
     2101 tcgatcgacg gcgatactga gcgtgtcgag cgcccggacg tccaggtagt cgcgcccgag
     2161 gcccgtcgtc tcgatcgccg gtccgccttc gcgcagcttc ggcgagacag gtccgccttc
     2221 gcgcggcttc ggcgggacag gcacgctcat gtccgcgccg gttcgagggt gatgtaccga
     2281 tacctatagt cgggagggtc ggccgcgtcc cgtatcgccg cgtcgatgac ggcgcgcgac
     2341 ggactcagcg tgcagacagg atccgtagcg gtggcatcgc cggtcagcgc gaacgcctga
     2401 cagcggcacc cgccgaaatc gatcgacttt ctcggacagc tccggcaggg ctcctgcatc
     2461 cactcgtcgc
//
LOCUS       DBNGSH010000719         5665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4322216, whole genome shotgun sequence.
ACCESSION   DBNGSH010000719 DBNGSH010000000
VERSION     DBNGSH010000719.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4322216"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2328
                     /locus_tag="AC16CY_16505"
     CDS             <1..2328
                     /locus_tag="AC16CY_16505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR3355452.1"
                     /translation="QNYVGLIEHALRRAMVPAWFGRGTRRPHPAGRAFLALLACRSEQ
                     LSASRFAEYLSFAQVPLAGVHPPPAWAASHDEAFGRVQEPVAQDDADAGEVTDGNGSE
                     PVVAGTLRAPWRWEGLLVDAAVIGQDATRWKRRLAGKYSELERHVREAKREDGGNQAR
                     VDGLRQVQAQLRHLEAFALPVIEEMSGWPSSATWGDWLDRFQALAPRVLRTPSHVMRV
                     LADLRPMGEVGPITLDEARRVLADRLLTLESEPPAQRYGRVFIGTPQQARGRSFRVVF
                     VPGLAERMFPQKPREDPLMLDTVREATGAPLPTQRQRLASERLLLQLAAGAASDRLYV
                     SYPRIELSESRARVPSFYALDVMRAATGQVPEHEVLEQQARTAGDATLAWPAPTDPAE
                     AIDNQEHDLAVLRRLLDDTSRGAVKGHAHYLLKLNECLRRSVVDQWARGERRWSPSDG
                     LTRVQPYTKAALAAQRLSVRSYSASALQLFTACPYQFVLSAMYRLRPFEPPEPLQRMD
                     PMTRGSLFHEIQAAFFHALTSLGALPVTATGLDEARAVLGAIVEEVGARAHDELAPAV
                     ERVWEDELSSIRRDLHAWLDHLAHDGEQWLPSYFEFGFGKVPGERDAHSVRDDVVLDG
                     RFRLRGAVDLIEEHTATKVLRVTDHKTGRKPERIERVTIGGGAVLQPVLYAMAVEAAL
                     GREVSHGRLFYCTAAGSFHEHGIPLDDRTRAAGLEVLEIVDRAIESGFLAAAPTVDAC
                     GRCDFRPVCGSNVVRRVARKPQDKLFDLIELRSRP"
     gene            2325..>5665
                     /locus_tag="AC16CY_16510"
     CDS             2325..>5665
                     /locus_tag="AC16CY_16510"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3355453.1"
                     /translation="MSPGTRAPDERARQLIHEALDETLVVEAAAGTGKTTELVQRIVR
                     ILAEGRADVREIVAVTFTEKAAGELKLRLRERLEQARASAGDVAGARLNEAVQNLEEA
                     HVSTIHGFCADLLRERPVEARVDPLFRVLTEGQAARLFNEAFEGWFQGVLAHPPEGVR
                     RSLRRSSRGARPGDVDEDAPMERLRRAGFDLAEWRDFRGAWTREPFDRRAAIPPVIDL
                     VHALADLSSSPSYAGDNLYVDTEPVRRLSRDLRRTVLTGSEEELDELESLLVDLRKNR
                     DVRRARKGTGPTYRKGATRAQVLDARDRLLQALDQFQIQADADLAALLHDELMACVDA
                     YEQLKLREGALDFLDLLVRARDLVKNDDAARQHFQQRFRRIFVDEFQDTDPLQAELLL
                     LLASRDPQETSWERVDPVPGKLFIVGDPKQSIYRFRRADVDVYRRVCEMLIARGATPV
                     ELRRSFRSVPNIQRAVNAAFDPLMDGDVETRQAKYVPLEPDRKDHAGQPSLVVLPVPE
                     PYGQRFIAAREIEQSLPDAVGAFVHWLVTESGWTVTERYGRRGRVPLEARHVCILFRR
                     FLSYGEDVTRPYVEALEARGVKHLLVGGKAFHGRDEIETLRAALMAVEWPDDQLSVFA
                     TLRGALFGIGDEELLEYHHLTGRFHPFRIPETPPSGLQPVCDGLRLLRALHTERNRRP
                     VSDTLAALLDFTRAHVGFVLRPGGEQVLANVLHVSELARRYEIEGGLSFRGFVEALQE
                     AAQGEQVAEAPILEEGSDGVRLMTVHKAKGLEFPVVILADITARLTPWDATRHVDADR
                     RVCALRIAGWSPKDLVDHKAIELAREQKEGVRIAYVAATRARDLLVVPAVGDEPYGEG
                     WVSPLNTAIYPQESDRRQQKAASGCPSFQSKDSVLTRPNGDPASRTTVCPGAHTIGAS
                     DDAHEVVWWSPEPGALTLGAQASFGLRRDDLIVKDVAPGVIRDRLEAYESWKAARTAD
                     RNAAARPTIEVMTATEAAMSLHDLPAERIEVVFAQASEGKSRPGGVRFGSLVHALLAD
                     VPLGEDGEPALARLSEAHGRILGAAPEEVAAAREAVAAALTHPVLAAAGRAARAGQCY
                     RETPVTIRLGTGAMVEGYVDLAYRD"
BASE COUNT          867 a         1919 c         2041 g          838 t
ORIGIN      
        1 cagaactatg tgggactgat cgagcacgcg ttgcgccgcg cgatggtgcc tgcgtggttc
       61 ggtcgcggca cccgccggcc gcatccggcc gggcgcgcgt tcctcgcgct gctcgcctgt
      121 cgatcggagc agctgtcggc ctcgaggttt gccgagtacc tgtcgtttgc ccaggtcccg
      181 ctggccggcg tccacccgcc gcctgcctgg gcggcttcgc acgacgaagc gttcggacga
      241 gtgcaagagc cggtcgccca ggacgacgcg gatgcggggg aggtcaccga cggaaacggc
      301 tccgagccgg tcgtggcggg aacgctgcgc gcgccgtggc gatgggaagg actgctcgtc
      361 gatgcggcgg tgatcggcca ggatgccacc cgatggaagc gccgtcttgc aggcaagtac
      421 agcgagctcg agagacacgt gcgcgaggcg aaacgagaag acggtggaaa ccaggcgcgc
      481 gtggacgggt tgaggcaggt acaggcgcag ctccgtcatc tcgaggcgtt cgcgcttccc
      541 gtgatcgagg agatgtcagg gtggccctcg tcggcgacct ggggcgactg gctcgatcgc
      601 ttccaggcgc tcgcgccgcg ggtcctgcgt acgccgtcgc acgtgatgcg cgtgctggcc
      661 gatctgaggc cgatgggcga ggtcgggccc atcacgctgg acgaggcgcg gcgcgtcctc
      721 gcggaccggc tgctgacgct cgaatcggag ccacccgcgc aacggtacgg ccgcgtcttc
      781 atcggcacgc cgcaacaggc gcgcggccgc agcttccgcg tggtgttcgt gcccgggctc
      841 gccgaacgga tgtttcccca gaagccgcgc gaggatccgc tgatgctcga caccgtgcgc
      901 gaggccacgg gagcgccgct gcccacccag cggcagcggc tcgcctcgga gcggctgctg
      961 ctgcagctgg cggccggcgc cgcctccgat cggctgtatg tctcgtaccc ccgcatcgag
     1021 ctgagcgaat cgcgcgcgcg cgtgccgtcg ttctacgcgc tcgatgtgat gcgcgcggcc
     1081 accggacagg tgcctgaaca cgaagtgctc gagcagcagg cgcgcacggc cggcgacgcg
     1141 accctggcct ggccggcgcc aaccgatccc gccgaggcca tcgacaacca ggaacacgac
     1201 ctggccgtcc tgcgccgcct gctcgacgac acctcgcgag gggccgtcaa gggacatgcc
     1261 cactacctgc tgaagctgaa cgagtgtctt cgccgttcgg tcgtggatca atgggcgcgc
     1321 ggcgagcggc gctggtcgcc gagcgacggc ctgacgcggg tccagccgta cacgaaggcg
     1381 gccctcgcgg cacagcgact gtcagtgcga tcctattcgg cctccgcgct gcagctcttc
     1441 acggcatgcc cgtaccagtt cgtgctctcg gcgatgtacc ggctgcggcc gttcgagccg
     1501 ccggagccgc tgcagcgcat ggatccgatg acgcgcggca gcctgtttca cgagatccag
     1561 gcggccttct tccacgcgct cacgtcgctc ggtgcgctgc ccgtcacggc gacaggtctc
     1621 gacgaggcgc gcgccgtgct cggcgcgatc gtcgaggagg tcggcgcgcg cgcgcacgac
     1681 gagctggctc cggccgtcga gcgtgtgtgg gaggacgagc tgtcgtcgat caggcgggac
     1741 ctgcacgcct ggctggatca tctggcgcac gacggcgagc agtggctgcc gtcctacttc
     1801 gagttcggct tcgggaaggt gccgggggag cgagacgcgc acagcgtccg cgatgacgtc
     1861 gtcctcgacg gccgattccg gctgcgcggc gccgttgacc tgatcgaaga gcacacggcc
     1921 acgaaggtgc ttcgcgtcac cgaccacaag accggacgaa agcccgagcg catcgagcgg
     1981 gtcacgatcg gcggcggtgc cgtgctgcag ccggtgctgt acgcgatggc cgtcgaagct
     2041 gctctcggcc gcgaggtcag ccacggcagg ctgttctact gcacggcggc tggaagcttt
     2101 cacgagcacg ggattcccct cgacgaccgc actcgggcgg cggggcttga ggtactggag
     2161 atcgtcgacc gcgccatcga gagcggcttc ctcgcggcgg ccccgaccgt ggacgcgtgc
     2221 gggcggtgcg acttcaggcc ggtgtgtgga tcgaacgtcg tccggcgcgt cgcgcgcaag
     2281 ccgcaggaca agctgttcga cctgatcgaa ttgaggagcc ggccgtgagc cccggtacgc
     2341 gagcgcccga cgaacgggca cgccagctca ttcacgaagc gctcgacgag acgctcgtcg
     2401 tcgaagccgc cgccgggacg ggcaagacca cggagctcgt ccagcgaatc gtccgcatcc
     2461 ttgccgaagg gcgcgccgac gtccgcgaga tcgtcgcagt cacgttcacg gaaaaggccg
     2521 ccggcgagct caagctgcgc ctgcgcgaac ggctcgagca ggcccgcgca tccgcaggcg
     2581 atgtcgcggg cgcgcgcctg aacgaggccg ttcagaacct cgaagaggca cacgtcagca
     2641 cgattcacgg cttctgcgcc gacctgcttc gagagcgccc ggtggaagcg cgggtcgatc
     2701 cgcttttccg cgtgctgacg gagggtcagg ccgcgcggtt gttcaacgag gcgttcgagg
     2761 gatggtttca gggcgtgctc gcacacccgc cggaaggcgt ccgccgctcg ctgcgccgat
     2821 cgagccgggg agcccgaccg ggcgacgtgg acgaagacgc gccgatggaa cggctgcgcc
     2881 gcgcgggctt cgatctggcc gagtggcgcg acttccgcgg cgcctggacg cgggagccct
     2941 tcgatcggcg agcggccatt ccgcccgtca tcgacctcgt tcacgcgctc gccgatcttt
     3001 cgtcgtcgcc ctcatatgcg ggcgacaacc tgtacgtcga caccgagccc gtccgccggt
     3061 tgagccgaga tctccgtcga acggtgctga ccggcagcga agaggagctc gacgagctcg
     3121 agtcgctgct cgtggatctc aggaagaacc gggacgtcag gcgcgcgcgc aagggaaccg
     3181 ggccgacgta ccgcaagggc gccacgcgcg cgcaggtcct cgatgcccgc gatcggctgc
     3241 tgcaggccct cgaccagttc cagatccagg cggacgccga ccttgccgcg ctgctgcacg
     3301 acgagctgat ggcctgcgtg gacgcgtacg agcagctgaa gctgcgcgag ggcgccctgg
     3361 acttcctcga cctgctggtt cgcgcgcgag acctcgtgaa gaacgacgac gcggcccggc
     3421 agcacttcca gcagcgattc cggcggatct tcgtggacga gttccaggac accgacccgc
     3481 tgcaggcgga gctgctcctg ctgctcgcct cgcgtgatcc gcaggagacg agctgggagc
     3541 gggtggaccc cgtacccggc aagctcttca tcgtcggcga tccgaagcag tcgatctatc
     3601 gcttccggcg cgccgacgtg gacgtgtacc ggagagtgtg cgagatgctc atcgcgcgcg
     3661 gcgcgacgcc cgtcgagctg cggcggagct tcaggagcgt cccgaacatc cagcgcgccg
     3721 tgaacgccgc attcgatccg ctgatggacg gagacgtcga gacgcggcag gcgaagtacg
     3781 tgccgctcga gccggaccgg aaggatcacg ccggccagcc ctcgctcgtc gtgctgccgg
     3841 tgcccgaacc gtacgggcag cgcttcatcg cggcgcggga gatcgaacaa tcgctgcccg
     3901 acgccgtcgg cgccttcgtc cactggctcg tgacggagag cggatggacg gtcaccgaac
     3961 gatacgggcg caggggacgg gtgccgctcg aggcgcgcca cgtctgcatc ctgttccggc
     4021 ggttcctcag ctacggggag gacgtcaccc ggccgtacgt cgaggcgctc gaggcgcgcg
     4081 gcgtgaagca cctgctcgtc ggcggcaagg ccttccacgg ccgcgacgag atcgagacgc
     4141 tgcgggcggc gctgatggcc gtcgaatggc ccgacgatca gctgtcggta ttcgccacgc
     4201 tgcgaggcgc gctgttcggc atcggtgacg aggagctgct cgagtaccac caccttacgg
     4261 gcaggttcca tccgttccgg attcccgaga cgcccccctc gggtcttcag cccgtgtgcg
     4321 acgggttgag gctgctgcgc gcgctgcaca ccgagcgcaa tcgccgcccc gtctcggaca
     4381 cgctcgcggc gctgctcgac ttcacgcgcg cccacgtcgg cttcgtgctg cggccgggag
     4441 gggaacaggt tctggccaac gtgctccacg tctccgagct cgcccgccgg tacgagatcg
     4501 aaggcggcct gtcgttccgg ggattcgtcg aggcgctgca ggaagcggcc cagggcgaac
     4561 aggtggccga ggcccccatt ctcgaggagg ggagcgacgg tgtccgcctg atgacggtgc
     4621 acaaggcgaa gggcctggag ttcccggtcg tgatcctggc cgacatcacc gcccgcctga
     4681 caccctggga cgccacgcgc cacgtggacg ccgaccgtcg cgtgtgcgcg cttcggattg
     4741 ccggatggtc tccgaaggac ctcgtggacc acaaagcgat tgagctggcg cgcgagcaga
     4801 aggagggcgt gcgcatcgcg tacgtcgcgg ccacgcgcgc gcgcgatctc ctcgtcgtgc
     4861 cggccgtagg cgacgagccg tacggcgagg gttgggtgtc tccactcaat acggcgatct
     4921 acccgcagga gagtgatcga cgtcagcaga aggcggcttc cggatgcccg tcgtttcaga
     4981 gcaaggactc tgtgctcacc cgccccaacg gcgatccagc gtcccggaca acggtctgcc
     5041 ccggcgcgca caccatcggt gcctccgacg atgctcacga ggtcgtgtgg tggtcaccgg
     5101 aacccggggc gctgacactc ggggcgcagg cctcgttcgg cctgcgccga gacgatctga
     5161 tcgtcaagga cgtggcgccg ggcgtcattc gcgatcggct ggaggcctac gagagctgga
     5221 aggccgcgcg cacggcagac cggaacgctg ccgcgcgccc gacgatcgag gtcatgacgg
     5281 caaccgaggc cgccatgtcg ttgcacgacc tgcctgcaga gcggatcgag gtcgtgtttg
     5341 cgcaggcatc ggaaggaaag agccggcctg gcggcgtcag gttcggatcg ctcgtgcatg
     5401 cgctgctcgc cgacgtacct ctgggtgaag acggcgagcc cgcactggcg cggttatccg
     5461 aagcgcacgg acggattctg ggcgccgcac ccgaggaggt cgccgccgcg cgcgaggccg
     5521 tggccgcggc attgacgcac ccggtgctcg cggccgcggg ccgtgccgcg cgggcggggc
     5581 agtgctaccg cgaaacgccg gtgacgatcc ggttgggcac gggcgccatg gtcgagggat
     5641 atgtcgatct cgcgtaccgt gacga
//
LOCUS       DBNGSH010000720         9328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3473901, whole genome shotgun sequence.
ACCESSION   DBNGSH010000720 DBNGSH010000000
VERSION     DBNGSH010000720.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3473901"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="fdxH"
                     /locus_tag="AC16CY_16515"
     CDS             <1..774
                     /gene="fdxH"
                     /locus_tag="AC16CY_16515"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015944 - formate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HIR3355454.1"
                     /translation="KLVDTSTCIGCKACEVACQEWNDLPPEATVQSGTYQTLPATTAH
                     FWNLIKFNEHVDEGRGALEWLMRKDQCMHCAEPGCLVACPAPAAIVQYSNGIVDFQQD
                     NCIGCGYCITGCPFDVPKFNAGTRRVYKCTLCVDRVSVGLQPACVKACPTSCLQFGTK
                     EDMLEVANTRVAQLKANGFAQAAVYDPPGVGGTSVITVLTHGDRPELYGLPKDPTIPA
                     AVRLWKGPLKWFGNAAMVGGLAAAVVHFVRFGRKDHAES"
     gene            771..1400
                     /locus_tag="AC16CY_16520"
     CDS             771..1400
                     /locus_tag="AC16CY_16520"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR3355455.1"
                     /translation="MTIHRIVRYDFRERVVHTIAALSYVYLLLTGLAFWTPWLFWIAI
                     ALGGGYVSRLLHPWAGLLFAAAVAAMYVQWRRDMRTTPEDREWRRAMAHYVRNEDGQV
                     PPAGRFNYGQKTLFWVMALGGLVLLLSGVVMWFVASVPSELHALRSVATILHAVAALV
                     TIGGFIVHIYMGVAVVPGGLRAIVHGDVTEAWARQHHGSWYRKMGSGVK"
     gene            1500..2288
                     /locus_tag="AC16CY_16525"
     CDS             1500..2288
                     /locus_tag="AC16CY_16525"
                     /inference="COORDINATES: protein motif:HMM:NF051162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355456.1"
                     /translation="MTPDPILAERIARARLLADRHPHAAGVLRFYAKLVEYQRALLDR
                     VPGIAFDREDLADLVPAFLDWLERESPPASWASAADLRRIAREDWRERFSQAIAPAGA
                     EADGGDSWTMFVVEALLQPLAERQAQDGRDRDSTTNEYEKSTGRCPACRDLPVVGVLR
                     EEGHGARRSLVCGRCATEWAYTRLACPGCGERQFESLPVYTAEQFGHIRVEACDSCRR
                     FLKTVDLTKDGLAVPLVDDLASLSLDLWARSQGYERLRPNLLRL"
     gene            2371..2967
                     /locus_tag="AC16CY_16530"
     CDS             2371..2967
                     /locus_tag="AC16CY_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355457.1"
                     /translation="MRRIRLYSILAAAMFTVAVVSISACHRTQPTPLPEYAPTATIKD
                     LMLSIVDPSADVVWLSVTTVQSAEGTVETAPKTDEEWKKVRQGAIALTEAANLLMMPG
                     RHVAGPGEKSETPGVELEPLEMEALIDKDRASWIMRAKGLHDAGMEVLQAIDAKDARK
                     VFEIGEHIERACESCHSQYWYPNEKIPPVPTELKQTNP"
     gene            3092..3856
                     /locus_tag="AC16CY_16535"
     CDS             3092..3856
                     /locus_tag="AC16CY_16535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3355458.1"
                     /translation="MRTALAALLSVAAGLIVTSGLVSAQHENVDIEDGGRAFRSACAN
                     CHGTAGDQIAGIDLGRGRFRRVYSDPELVGIIRNGIPNTPMAATGVTEQQAVNIVGYL
                     RSLAGSTRGPAVVGDSTRGRSIYDGKGACATCHRVNGVGSRVGPDLSAIGQNRQSAEL
                     EQSLVDPGAEVLATNRFYRVVTKEGVAVMGRLLNHDTFTVQILDSKEQLRSFVKADMR
                     EHGFVGTPMPSYFGTLDPQERADVVSYLMSLKGGSR"
     gene            3853..5460
                     /locus_tag="AC16CY_16540"
     CDS             3853..5460
                     /locus_tag="AC16CY_16540"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR3355459.1"
                     /translation="MTARTVVLVLLAAVSLQGQVTFNRILNADREPHNWLSYSRTLDN
                     QRFSPLTQITSANVKNLQLQWVWQSRSLEKFEATSLVVDGVLYTVQQPNDVVALDAVT
                     GRVYWTLTYQPAPEARSCCGRVNRGLAISGDTLFMGTLDAHLLAIDARSGQVIWNTTV
                     ADTKARYVITHSPLVVRDKVIIGTAGGDGPIRGLIAAFDIKTGKELWRWYTIPAPGEP
                     GNETWAGDSWMIGGVGVWNAGAYDPETNLAFWGTGNPSPGMDGRTRRGDNLYSDSVVA
                     LDVDTGKLKWHYQFTPHDEMDYDSTQVPLLADITFRGKPRKVMLWANRNGLMYVLDRV
                     TGEFLFGKPYVKVNWMDGFDEKGRPKRVPGMVPGPEPKLIQPHIHGASNWAPPAFSPR
                     TGLFYVSHWENSGSYVGQGRFARPAGVNTRQTGMGQVNLLPFFNNENETFGVVRAYDP
                     NTLEPTWEYRMTDITWGGVLATAGDLVFSGGREGYFFALDARTGALLWKASVGGQVNA
                     GPMSYAVGGRQFVSIAAGSALFTYALP"
     gene            5518..6465
                     /locus_tag="AC16CY_16545"
     CDS             5518..6465
                     /locus_tag="AC16CY_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355460.1"
                     /translation="MRLLGAVAFVTAASVAAAAQSPSYRAPRTPDGQPDLQGFWSNAT
                     YTPLERPDNVTKAFYTPEEAQAAEKRAAAAENAQTEPGTVADVHYDFTQFGLDRSQAA
                     RARNLRTSLIVDPPNGKLPPVTAEGKRILAARAQAAKLLGGRWDSAQSNQLDDRCLIM
                     AGAGPPMMDAAYNSNYHIVQAPGYVMILTEMIHDARIIPLDGRPQPSENVRQWMGVSR
                     GRWDADTLVIETTNFNGKNPLRGSTEHMKVTERLRRVADDVIEYKFTVDDPRMWASAW
                     SAEAVMKKTIGPIFEHACHEGNYGLYNTLVGARLEEKKK"
     gene            6512..7330
                     /locus_tag="AC16CY_16550"
     CDS             6512..7330
                     /locus_tag="AC16CY_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355461.1"
                     /translation="MRNLNATRFTAMAALCTGVVLAATLVLAQQPAGYRAPRTPDGRP
                     NLNGIWQAINTAHWDLEPHSPSPSPVLELGAAGAVPGGLGVVEGGVIPYRPEALAKRN
                     ENRAGRLKLDPEVKCYLPGVPRATYMPFPFQIIQSQKHIMLIHEFAGAVRTIYMTDQV
                     EAPADSWMGWSNGRWDGESLVVDTKGFNDLSWFDRAGNFHSDALHVVERFTARSADTL
                     NYEATIEDPKTFTKPWKISMPLYRRVEPNAQIMEFRCVEFAEELLYGHLKKQPK"
     gene            7404..8435
                     /locus_tag="AC16CY_16555"
     CDS             7404..8435
                     /locus_tag="AC16CY_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355462.1"
                     /translation="MNRRTLVSIGLLTLAVAVILSATPAAQAPAASPKAGAAAKAYTP
                     PKTPDGQPDLQGFWTNSTYVPLERPNNVTKEFYTPEEALAAEKRAAEREATQTEPGTT
                     ADVHYDFTQFGLDRSQATISRNLRTSLVIDPPDGRIPPVNATGQKRAAEVAAARKTRG
                     GQYDSVHNMPIGSRCIIMGGAGPPMMNAGYNANYQIVQGPGYVMILTEMIHDARIIPL
                     DGRPAPPAGARQWTGISRGRWEGNTLVVETTNFNGKNPFRGASENMKVTERFTRVSDD
                     VLDYKFTVEDPATWDRSWTAEAPLAKTQGPIFEFACHETNYGVANILAGAREDERRAA
                     EAAVKKGSN"
     gene            8474..8884
                     /locus_tag="AC16CY_16560"
     CDS             8474..8884
                     /locus_tag="AC16CY_16560"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355463.1"
                     /translation="MRAKLAVAIACAGLFLTAGHVRAHHAFAAEFDVNKPLTLKGSLL
                     KWEMVNPHSWFHLTVKEPDGKVSTWLVEGGSPNQLIRMGVTKNTVPIGTELTVEGYRA
                     KDGTLKAVGRNFVLADGSRLFLGGSAPGGGGDKK"
     gene            complement(8911..>9328)
                     /locus_tag="AC16CY_16565"
     CDS             complement(8911..>9328)
                     /locus_tag="AC16CY_16565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3355464.1"
                     /translation="GVATKRHGSALHPAALNAPAMFIGALVLAAASLASGDGLRLPRD
                     PGAWGAILYLAIAGTVVTFLIYFSLLKTWSVTSLSFISVFTPAIALALGFVFLDERLT
                     LWTGLGAALILAGVALSVMRERTVQGGSEDPPLHGR"
BASE COUNT         1706 a         2981 c         3152 g         1489 t
ORIGIN      
        1 aagctcgtgg acacgtccac ctgcatcggc tgcaaggcgt gcgaggtggc gtgccaggag
       61 tggaacgatc tgccgcccga ggcgacggtg cagtccggca cgtaccagac gctgccggcc
      121 acgaccgccc acttctggaa cctgatcaag ttcaacgagc acgtggacga gggccgcggc
      181 gcgctcgagt ggctgatgcg gaaggaccag tgcatgcact gcgccgagcc gggctgtctc
      241 gtcgcgtgcc ccgcgcctgc cgccatcgtc cagtactcga acggcatcgt cgatttccag
      301 caggacaact gcatcggctg cggatactgc atcaccggat gcccgttcga cgtgccgaag
      361 ttcaacgcgg gcacgaggcg cgtctacaag tgcacgctgt gcgtcgatcg cgtatcggtg
      421 gggctgcagc ccgcgtgcgt gaaggcatgt ccgacgagct gcctgcagtt cggcaccaag
      481 gaggacatgc tcgaggtggc caacacacgc gtcgcgcagc tcaaggccaa cgggtttgcc
      541 caggccgccg tctacgaccc gccgggcgtc ggggggacct cggtcatcac ggtactgaca
      601 cacggcgatc ggccggagtt gtatgggtta ccgaaagacc cgacgatccc ggctgccgtc
      661 cggctgtgga aggggccgct gaagtggttc ggcaacgcgg cgatggtggg tggactcgcc
      721 gccgccgtcg tgcacttcgt gcgcttcgga cgaaaggatc acgccgagtc atgaccatcc
      781 accgcatcgt gcgttacgac ttccgcgagc gggtcgtcca cacgatcgcc gcgctctcgt
      841 acgtctatct tctgctgacg gggctggcgt tctggactcc gtggctcttt tggatcgcga
      901 tcgcgctcgg cggcggctat gtgtcgcgtt tactccaccc ctgggccggg ttgctcttcg
      961 ccgcagccgt cgccgcgatg tacgtccaat ggcgtcgcga catgcggacc acgcccgagg
     1021 accgcgagtg gcgtcgcgcg atggcgcact acgtccggaa cgaagacggg caggtacctc
     1081 cggccggccg cttcaactac ggtcagaaga cgctcttctg ggtcatggcg ctcggcggcc
     1141 tcgtcctgct cctgtccggc gtcgtcatgt ggttcgtggc gtcggtcccc tcggagctgc
     1201 acgcactgcg gtcggttgcc acgatcctcc acgccgttgc cgcgctcgtc accatcggcg
     1261 gcttcatcgt gcacatctac atgggcgtcg ccgtcgtgcc cggcgggctt cgtgcgatcg
     1321 tacacggcga cgtcaccgaa gcgtgggcac gccagcacca tggcagctgg taccggaaaa
     1381 tggggtcggg agtcaagtaa atggggtcgg gagtcattta ttgagcgcac tggcggatcg
     1441 acgtcaccag gtccccgccc tcaataaatg actcccgacc ccagttacgc cgccgataaa
     1501 tgactcccga ccccattttg gccgagcgca tcgctcgcgc tcggctgctc gccgacaggc
     1561 atcctcacgc cgcgggggtc ctgagattct acgcaaagct cgtggagtac cagcgcgcgc
     1621 tgttggaccg cgtgccgggg atcgctttcg atcgagagga tctcgcagac ctcgttcctg
     1681 cctttctgga ttggctggag cgcgagagtc ccccggcgtc gtgggcgtcc gctgcagatc
     1741 ttcggcggat cgcgcgtgaa gactggcgag agcgattcag ccaggcgata gcgcccgcgg
     1801 gcgctgaagc cgacggcggc gattcgtgga cgatgtttgt cgtcgaggcg ctgctgcaac
     1861 cccttgcgga gcggcaggcg caagacggcc gtgaccgcga ttcgacgacg aacgaatacg
     1921 agaagagtac tgggcggtgt cccgcctgcc gcgaccttcc cgtggtcggg gtgctgcgtg
     1981 aagaagggca cggcgcccgg cgctcgctgg tgtgcgggcg ctgcgccacc gaatgggcgt
     2041 acacgcggct ggcgtgcccg ggctgcgggg agcgccagtt cgaatcgctg cccgtctata
     2101 cggccgagca attcggccac atccgtgtcg aggcctgcga cagctgccgg cggtttctca
     2161 agacggtgga cctcacgaaa gacggcctgg ctgtgccgct ggtggacgat ctggccagcc
     2221 tttcgctcga cctctgggcc cgctcgcagg ggtacgaacg ccttcggccg aacctgttga
     2281 gactctgaac actctgaaga agaattcagc aagacttcag gattacgtac ggccgggcct
     2341 cgtacagtcg aagagaaagg aaggcagttc atgcgaagaa tccgcttgta ttccattctc
     2401 gccgccgcga tgttcacggt tgcggtcgtg tcgatcagcg cgtgccatcg gacgcagccg
     2461 acgccgctgc cggaatatgc gccgacggcc accatcaagg atctgatgct gtcgatcgtg
     2521 gatccgtcgg ctgatgtcgt gtggctctcg gtcaccacgg tccagagcgc ggaggggacg
     2581 gtcgagaccg cgccgaagac cgacgaagag tggaagaagg tccggcaggg cgcgatcgcg
     2641 cttaccgagg cggccaacct gctgatgatg cccggccgtc acgtggccgg tccgggcgag
     2701 aagtccgaga cgcccggcgt cgagctcgag ccgctcgaga tggaggcgct catcgacaaa
     2761 gatcgggcga gctggatcat gcgggcgaag gggctgcacg atgccggcat ggaagtgctg
     2821 caggcgatcg atgccaagga cgccaggaag gtcttcgaga tcggcgagca catcgagagg
     2881 gcgtgcgaga gctgccactc gcagtactgg tacccgaacg aaaagatccc acccgttccg
     2941 accgaactga agcagacgaa cccgtaggac gcggcagctc gtcctcgaaa accagaggaa
     3001 aaccgtcacc ttcggcccgt cagcgcactg atccggcggt tgatctcccg cgcgcgggca
     3061 gctaagattt cggccagctt cacacgccaa catgcgtacc gcgctcgcgg cgcttctctc
     3121 ggtggcagcc ggcctcatcg tgacctcggg tctggtgtct gctcagcacg aaaacgtcga
     3181 tatcgaggac ggtggccgcg cgtttcggag cgcatgcgcg aactgccacg gcactgccgg
     3241 cgatcagatc gccggcatcg acctcggccg cgggcggttc cggcgtgtct actccgatcc
     3301 ggagctggtg ggcatcatcc gcaacggcat cccgaacacg ccgatggcgg caactggggt
     3361 gaccgagcag caggccgtca acatcgtggg ctaccttcgg tcgcttgcgg gctcgacgcg
     3421 aggccccgcc gtcgtgggcg actcgacacg gggcagatcg atctatgacg ggaaaggcgc
     3481 ctgcgcgacg tgtcatcgcg tgaacggggt cggctcgcgc gtgggtcccg acctgagtgc
     3541 gattgggcaa aaccggcaga gcgccgagct ggagcagtcg ctcgtggacc ccggggctga
     3601 ggtcctcgcg acgaaccggt tctaccgcgt cgtcacgaag gagggcgtcg cggtgatggg
     3661 ccggctgctc aaccacgaca cgttcaccgt tcagattctc gactcgaagg aacagctgcg
     3721 gtcgttcgtg aaagcggaca tgcgggagca cggtttcgtg gggacgccga tgccctcgta
     3781 tttcggcaca ctcgatcccc aggagagagc cgacgtggtc agctacctca tgtcgctcaa
     3841 ggggggttcc cgatgacggc gcgaaccgtc gtgctcgtgc tcctggccgc cgtgtcactc
     3901 caggggcagg tgaccttcaa ccgcatcctc aacgcggacc gcgagccgca caactggctc
     3961 agctactccc gcacgctcga caaccagcgt ttcagcccgc tgacgcagat cacgtccgcc
     4021 aacgtgaaga acctgcagct ccagtgggtc tggcagtcgc gttcgctgga gaagttcgag
     4081 gcgacgtccc tggtcgtgga cggcgtgctc tatacggtgc agcagcccaa cgacgtggtg
     4141 gcgctggacg cggtgacggg ccgggtctac tggacgctca cgtatcagcc ggcgccggag
     4201 gcgcgcagct gctgcgggcg cgtcaatcgc gggctcgcga tttctggcga cacgctgttc
     4261 atgggcaccc tcgatgcgca cctgctggcg atcgacgcca ggagcggtca ggtgatctgg
     4321 aacaccacgg tggccgacac gaaggcgcgc tacgtgatca cgcactcgcc gctcgtggtc
     4381 cgcgacaagg tcatcatcgg cacggcgggc ggcgacggcc cgatccgcgg cctgatcgcg
     4441 gccttcgaca tcaagacggg caaagagctc tggcgctggt acacgattcc ggccccggga
     4501 gagcccggca acgagacgtg ggccggcgac tcctggatga tcggcggcgt tggggtgtgg
     4561 aatgcgggcg cctacgatcc cgaaacgaac ctggccttct ggggcacggg caacccgtcg
     4621 cccggcatgg acggacggac gcgtcgcggc gacaacttgt acagcgacag cgtcgtcgca
     4681 ctcgacgtcg ataccggaaa gctgaagtgg cactaccagt tcacgccgca cgacgagatg
     4741 gactacgact ccacgcaggt gccgctgctg gccgatatca ccttccgcgg caagccgcgc
     4801 aaggtcatgc tgtgggccaa tcgcaacgga ctgatgtacg tgctggatcg cgtcaccggc
     4861 gagttcctgt tcggcaagcc gtacgtgaag gtcaactgga tggacggctt cgacgagaag
     4921 ggacggccga agcgtgtgcc cggcatggtg ccgggccccg agcccaagct catacagcct
     4981 cacatccacg gcgcgtccaa ctgggcgccg cctgccttca gtccgaggac cggcctgttc
     5041 tacgtctcgc actgggagaa ctccggttcc tatgtcgggc aaggccgttt tgcgaggccg
     5101 gcgggcgtga acacgcgcca gaccgggatg ggtcaggtca acctgcttcc cttcttcaac
     5161 aacgagaacg agacgttcgg cgtcgtgcgc gcctacgatc ccaacacgct cgagccgacg
     5221 tgggagtacc gcatgacgga catcacgtgg ggcggcgtgc tggctacggc cggcgacctg
     5281 gtgttcagcg gcgggaggga agggtacttc ttcgcgctcg acgccaggac tggggcgctg
     5341 ctgtggaagg cgtcggttgg tggccaggtc aacgccggtc cgatgagcta tgcagtcggc
     5401 ggtcgccagt tcgtctcgat cgcggcgggt agcgctctgt tcacctatgc gcttccctag
     5461 gcagtatgcg tacgtagtgt ccggcttcag ccggaccagc aggcagtggg cgcgacgatg
     5521 agacttctcg gtgcggtggc atttgtgacg gcggcgtccg tggctgcggc tgcgcagtcg
     5581 ccatcgtatc gcgcgccgcg gacgcctgac ggccagccgg atcttcaggg gttctggtcg
     5641 aacgccacct acacgccgct cgagagaccc gacaacgtga ccaaggcgtt ctacacgccg
     5701 gaggaagcgc aggccgccga gaagcgagcg gccgcggccg aaaacgcaca gacggagccg
     5761 ggcacggtgg cagacgtgca ctacgacttc acgcagttcg ggctcgaccg aagccaggcc
     5821 gcgcgcgcca ggaacctgcg cacctcgctc atcgtggatc cgccgaacgg aaagcttccg
     5881 ccggtgactg ctgaaggaaa gcggatcctt gcggcgcgcg cccaggccgc gaaactgctg
     5941 ggcggccgct gggactccgc gcagagcaat cagctcgacg accgctgcct catcatggcg
     6001 ggcgcggggc cgccgatgat ggatgccgcc tacaacagca actatcacat cgtgcaggcg
     6061 ccggggtacg tgatgatact caccgagatg attcacgacg cccgcatcat ccctctggat
     6121 ggtcgaccgc agccctccga gaacgtccgg cagtggatgg gggtctcccg cggacgctgg
     6181 gatgccgaca cgctcgtcat cgagaccacc aacttcaacg gcaagaatcc cctgagaggc
     6241 tcgaccgagc acatgaaggt caccgagcgg ctcaggcgtg tggccgacga cgtgatcgag
     6301 tacaagttca cggttgacga tccccggatg tgggcgagcg cgtggtcggc ggaggccgtg
     6361 atgaagaaga ccatcggccc gatcttcgag cacgcctgcc acgaaggaaa ctacgggctc
     6421 tacaacacgc tcgtcggcgc gagactcgaa gagaagaaga agtagtgtcc ggctttatcc
     6481 ggaccgtgga gaagaagggc tctcgaaata aatgcggaac ctcaacgcga ctcgattcac
     6541 ggcgatggcg gccctgtgca ccggcgtggt gctggccgca acacttgttc tcgcgcagca
     6601 gccggctggt tatcgcgccc cgcgcacgcc ggacggcagg ccgaacctca atggcatctg
     6661 gcaggcgatc aacaccgccc actgggatct cgagccgcat tcgccttcgc cgagcccggt
     6721 actggagctc ggcgcggccg gggcagtgcc aggcggcctg ggcgtggtcg aagggggcgt
     6781 catcccgtac cggcctgagg cgctggccaa gcggaacgag aatcgggcgg gccggttgaa
     6841 gctcgacccc gaggtgaagt gctacctccc gggagtgcca cgggcgacct acatgccgtt
     6901 cccgttccag atcatccaga gccagaagca catcatgctc attcacgagt tcgcgggcgc
     6961 ggttcgcacg atctacatga cggatcaggt cgaggcccct gcggacagct ggatgggatg
     7021 gtccaatgga cgttgggacg gggaatcact cgtcgtcgat acgaagggat tcaacgactt
     7081 gagctggttc gaccgggcgg gcaatttcca cagcgatgct ctccacgtgg tcgagcgttt
     7141 tacggcccgg agcgccgata cgctcaatta cgaggcgacg atcgaggacc cgaagacctt
     7201 tacgaagccc tggaagatca gcatgcccct ctatcgacgc gtcgagccaa acgcgcagat
     7261 aatggagttc cggtgcgtgg agtttgcgga ggagctgctg tacgggcatt tgaagaagca
     7321 gcccaagtag ccggtggcag gcagtaggcc gtaggcagtc gtagagcgga ggcttcagcc
     7381 tccgcggtga aatggagagg cagatgaatc gtcgaactct cgtttcgatt ggattgttga
     7441 ctctggcggt tgcggtgatc ctgtcggcca cgcccgccgc acaggcgcca gccgcatcac
     7501 cgaaggccgg cgccgcggcc aaggcgtaca cgccgccgaa gacgcctgac ggtcagccgg
     7561 acctgcaggg cttctggacc aattccacct acgtgccgct cgagcggccc aacaacgtga
     7621 ccaaggagtt ctacacgccc gaggaagcgc tcgcggctga aaagcgagcc gccgaacgtg
     7681 aagccactca gactgaaccc gggacgactg ccgacgtgca ctacgacttc acgcagttcg
     7741 gcctggaccg cagccaggcg accatctcgc ggaacctgcg cacgtcgctc gtgatcgatc
     7801 cgccggatgg caggattccc ccggtcaacg ccacggggca gaagcgtgcc gccgaggtgg
     7861 ctgccgcgag gaagacccgg ggaggccagt acgactcggt ccacaacatg ccgatcggat
     7921 cgcgctgcat catcatgggc ggggccggac ctccgatgat gaacgcgggc tacaacgcga
     7981 actaccagat cgtccagggg ccgggctacg tgatgattct gaccgagatg attcacgacg
     8041 cccgcatcat cccgctcgac ggtcggccgg ctccgcccgc gggcgcgcga cagtggaccg
     8101 gaatctcgcg cggccgctgg gagggcaaca cgctggtcgt cgagacgacg aacttcaacg
     8161 gaaagaaccc gttcaggggc gccagcgaga acatgaaggt caccgagcgc ttcacgcgcg
     8221 tatccgacga cgtgctcgac tacaagttca cggtcgagga tccggcgacg tgggatcgca
     8281 gctggacggc cgaagcaccg ctggcgaaga cgcagggccc gatcttcgag ttcgcatgcc
     8341 acgagaccaa ctacggcgtc gcgaacatcc tggcgggtgc gcgggaggac gagcgacggg
     8401 ccgccgaggc cgccgtcaag aagggttcga attgagtaga gcgggggctt cagcccccgc
     8461 ggaggtcaat ccaatgcgcg caaagcttgc cgtcgcgatt gcctgcgccg gcctcttcct
     8521 gactgcgggg cacgtgcggg cgcatcacgc gttcgcggcc gagttcgacg tgaacaagcc
     8581 gctcacgctc aagggatcgc tcctcaagtg ggagatggtc aacccgcact cgtggttcca
     8641 cctcaccgtc aaggagccgg acgggaaggt cagcacgtgg ctcgtcgaag gcggcagccc
     8701 gaatcagctg attcgaatgg gcgtcacgaa gaacacggtg ccgatcggca ccgagttgac
     8761 cgtcgaaggc taccgcgcca aggacgggac cttgaaggcg gtcggcagga acttcgtcct
     8821 cgccgacggg tcgcgcctgt ttctcggcgg ctcggccccc ggaggcggcg gagataagaa
     8881 gtaagggacg gcgggtctca agacccgccg ctacctcccg tgtaggggcg ggtcttcaga
     8941 cccgccctga accgtccgct cccgcatcac ggacagcgcg acccctgcga gaatgagcgc
     9001 cgccccgagc ccggtccaga gcgtgagccg ctcgtcgaga aacacgaatc cgagcgcgag
     9061 cgcgatcgcg ggcgtgaaca cgctgatgaa actcaggctg gtcacgctcc atgtcttcag
     9121 cagcgagaaa tagatcagaa acgtcaccac tgtcccggcg atcgccaggt acagaatggc
     9181 tccccacgct cccggatcgc gcggaaggcg caggccgtcg cccgaggcca gcgacgccgc
     9241 ggccagcacg agcgcgccga tgaacatggc cggcgcgttg agcgctgccg gatggagcgc
     9301 cgagccgtgc cgcttcgtcg cgacgccc
//
LOCUS       DBNGSH010000721         1512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1435870, whole genome shotgun sequence.
ACCESSION   DBNGSH010000721 DBNGSH010000000
VERSION     DBNGSH010000721.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1435870"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..>1512)
                     /locus_tag="AC16CY_16570"
     CDS             complement(105..>1512)
                     /locus_tag="AC16CY_16570"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3355465.1"
                     /translation="LRALVSIDLPLPYGLRLDLNFGIDSTVLFGWRVLALTAGAGVLT
                     GVVLGLVPAAQGTRADLGSALKTGSRGSDAPGTLRWRNALVIAQVAMSLVLLVGAGLF
                     LRSWQQMLSVDPGFGRAPTSILALMMPIARSTPDEVVQRTRRVLERFRALPGVEAAGL
                     VWPLPLEFASSFTDFTVDGGVPPPGQESYRANRATVDGAFFDAAGVAIVAGRTFNDGD
                     RRDTQPVAVISQAMARRYWPDGDALGRVLRRPDPADTDLMVVGVASDINVRSLGEAPR
                     DLVYENYAQSNASPIFNFVVRAATDPARITSALIAAVPEIDADLQIVQSTTMAEHLAM
                     SRLPSQLGAFMLSAFAVMAVALAAVGVYGLVRYTVAMRTREVGIRMALGADAAGVARL
                     LATNGLRLVLVGGAIGVAASLVAARFLAALLVGVGTFDPVALTGAPLVLGAAAWLAAY
                     LPARRASRVDPLAALRSE"
BASE COUNT          270 a          519 c          530 g          193 t
ORIGIN      
        1 tgaaactcga gttcgtcctt cagatcagtg tcgaatcggc ggcggcgcaa caggcttgca
       61 agccacaggc ggatgcgcgt ccacatggcg gtacacccca ggaatcactc gctgcgaagc
      121 gcggcgagcg ggtccacgcg gctggcgcgc cgcgcgggca ggtacgcggc cagccacgcc
      181 gcggcgccga gcacgagcgg cgcacctgtc agtgcgacgg gatcgaacgt cccgacgccg
      241 acgagcagcg ccgcgaggaa gcgagccgcg acaagcgatg cggcaacgcc gatggccccg
      301 ccgaccagca ccagccgcag gccgttggtc gcgagcagcc gcgcgactcc ggcggcatcg
      361 gccccgagcg ccatgcgtat gccaacctcg cgagtacgca tcgccacggt gtatcgcacc
      421 agcccgtaca cgccgacggc ggcaagcgcc acggccatca ccgcgaacgc cgacagcatg
      481 aaggcgccca gctgcgacgg cagacgcgac atcgccagat gctccgccat cgtcgtggac
      541 tggacgatct gcaggtccgc atcgatctcc ggaacggcgg caatcagcgc tgacgtgatc
      601 cgggccggat cggtcgctgc gcggacgacg aagttgaaga tggggctcgc gttactctgc
      661 gcgtagttct cgtagaccag gtcgcgcggt gcttctccga gcgagcgcac gttgatgtcg
      721 ctggccacgc cgacgaccat caagtcggtg tcggccggat cgggtcggcg caagacgcgg
      781 cccagcgcgt cgccgtcggg ccagtaccgg cgggccatgg cctgactgat gacagccacg
      841 ggttgcgtgt ctcgccgatc gccgtcgttg aaggtgcggc cggccacgat ggccacgccg
      901 gctgcgtcga agaaggctcc gtccaccgtg gcccggttgg cgcggtacga ctcctggccg
      961 gggggcggga cgcctccgtc gacggtgaag tccgtgaagc tcgaggcaaa ctcgagcggc
     1021 agcggccaga cgagcccggc ggcttctacg ccgggcagcg cccgaaagcg ctcgagcacg
     1081 cgacgcgtcc gctgcacaac ttcgtcggga gtggaacgcg cgatcggcat catgagggcg
     1141 aggatcgacg tcggagcccg gccgaagccg ggatcgacgg agagcatctg ctgccagctt
     1201 ctcaggaaca gcccggctcc gacgagcagc acgagggaca tggcgacctg ggcgatgacc
     1261 agtgcgttgc gccatcggag tgtgcccggc gcatcgcttc ctctgctgcc tgtcttgagc
     1321 gccgagccga ggtcggcgcg cgttccctgc gcggcgggaa ccagtccgag gacgacccct
     1381 gtcaacacgc ccgcgcccgc agtcagtgcc aggacccgcc aaccgaagag caccgtcgag
     1441 tcgatgccga agttcagatc gagcctcaaa ccatacggca gcgggaggtc gatcgacacc
     1501 agagcacgga gg
//
LOCUS       DBNGSH010000722         3080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3304325, whole genome shotgun sequence.
ACCESSION   DBNGSH010000722 DBNGSH010000000
VERSION     DBNGSH010000722.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3304325"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3080
                     /locus_tag="AC16CY_16575"
     CDS             <1..>3080
                     /locus_tag="AC16CY_16575"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3355466.1"
                     /translation="VPTVVFAQATITGTVRDSSGGVLPGVTVEATSPVLIEKVRTAAT
                     DGSGQYRIIELLPGTYTVTYTLPGFNRVVRDNVEVAGSGIVTINADMRVGALEETITV
                     TSETPVVDLQSVRRETVMSSEVIEAIPATRAVGSLLNATPGLTVDNNGMAPTPTMTFF
                     SARGGQTNEGRMTVNGMPVAATFNGGGVSSYIYDSVNADEVSVIVSGSLGETDVGGPV
                     MNLVPRAGGNSFRGEAFYNGSGDALRGNNLTDELMAPAPGPNLRETPGIINSWDASVS
                     YGGPIKRDRFWFFGSYRNLETATAREGIVANANAFDYSHWDWAPDNSLTARRIEGRSM
                     IIGRFTAQATEKNRISFNHEYQLRCEGSPLKVETTTGCNTRGDDWIALGASGIVSSSP
                     EADTGYFDMPYYVTQALWTNPMTNKLLLEAGYTRFSYYHAGGPGQLPPDGIFDQIAVS
                     EQSTAVNPATGLQYAPRANYNYRALATYSDNYGKSDLWRASASYVTGAHNMKIGYQGA
                     YLLNDTTAVIPDSQVSYRFSQGSPNAFTMRLPTWNQAERTQTASIFIQDAWTRGRLTL
                     QGALRYDRAWSFSPAEHNGTELTSKFNAAPISFPRTVGVDSFNDITPRVGVAYDVFGN
                     GKTALKFNWGHYLDAATNDSAYTRNSPAQNTVRTVTNRGWTDHDGDKVIDCNPMNFAA
                     QPGAPGTGLDTCAALTGNDLNFGSTSANVTQVNQATLRGWGVRENDWQWGLTVQQEIV
                     PRVSIDVGYNRRWWHGFTVQDNTVRTPDEYTAWTINAPVDPRLPGGGGYPITMYTVTA
                     AASAEAARNYVTFETDFGPERTNYWHGVDVNVSARLRNGLFLSAGTSTGREIENDCAT
                     QVKIDSPDDRMCNSIEPFQTTVRGLATYTIPKIDLLVSGTLRSQPPLGRVATWNVPNT
                     LVSQPQYLGRVPPGSTINGNTAVTLFDNNEYRLYADERRTQLDIRIAKILRFGPTRAD
                     VGVDIGNVFNTNYATAYENTYQYSVGNAGMGGTWNNPTSIYTPRYFRINTTVSF"
BASE COUNT          596 a         1028 c          997 g          459 t
ORIGIN      
        1 gtgcccacgg tggtgttcgc gcaagccacc atcacgggca cggtccgaga cagctcgggc
       61 ggggtgctgc cgggagtcac cgtcgaggcg acgagcccgg tcctgattga aaaagtgcgg
      121 acggcggcca cagacggatc cggtcagtac cgcatcatcg aactccttcc cggcacctac
      181 acggtgacct acacgctgcc cgggttcaac cgggtggttc gcgacaacgt cgaggtggct
      241 ggcagcggca tcgtcaccat caacgccgac atgcgcgttg gcgcgctgga agaaaccatc
      301 accgtgacca gcgagacacc ggtcgtggac cttcagagcg tccgccgcga gacggtgatg
      361 agctccgagg tcatcgaagc gatcccggcg acccgcgccg ttggctcgct gctcaatgcc
      421 acgcccggcc tgacggtcga caacaacggc atggccccga cgccgacgat gacgttcttc
      481 agcgcgcgcg gtgggcagac caacgaaggg cgcatgaccg tcaacggcat gcccgtcgct
      541 gcgaccttca acggcggcgg cgtgtcgtcg tacatctacg actcggtgaa tgccgacgag
      601 gtttcggtga tcgtgtcggg ttcgctgggt gaaaccgacg tgggcgggcc ggtgatgaac
      661 ctggttcccc gcgcgggcgg caactcgttc cgcggcgagg cgttctacaa cggctcgggc
      721 gacgcgttga ggggcaacaa cctcacggac gagttgatgg cgcccgctcc ggggcccaac
      781 ctgcgcgaga ccccgggcat catcaattcc tgggatgcga gcgtgtcata cggcggtccg
      841 atcaagcgcg atcggttctg gttcttcggc agctaccgca acctcgagac ggcgacggcc
      901 cgggaaggga tcgtcgccaa cgcgaacgcc ttcgactact cacactggga ctgggctccc
      961 gacaacagcc tgacggcgcg ccggattgaa gggcggtcga tgatcatcgg gcgcttcacc
     1021 gcgcaggcca cggaaaagaa ccgcatctcg ttcaaccacg agtaccagct ccggtgcgag
     1081 ggttcgccct tgaaggtcga gacgaccacc ggctgcaata cccgcggcga cgattggatc
     1141 gcgctggggg cctcgggcat cgtctcgtcc tcgcccgaag cggatacggg gtacttcgac
     1201 atgccgtatt acgtcaccca ggcgctctgg acaaacccga tgaccaacaa gctgctgctc
     1261 gaggcgggct acacgcggtt ctcgtactac cacgcagggg gccccggcca gcttccgcct
     1321 gacgggatct tcgaccagat cgcggtgtcg gagcagtcga ccgccgtgaa cccggcaacg
     1381 gggctgcagt acgcgccacg agcgaactac aactaccgcg cgcttgccac ttacagcgac
     1441 aactacggca agtcggacct ctggcgcgcg tcggcctcgt acgtgactgg tgctcacaac
     1501 atgaagatcg ggtaccaggg cgcgtatctc ctcaacgata cgaccgccgt gattccggat
     1561 tcgcaggtct cctaccgttt cagccagggg agcccgaatg cgttcacgat gcggctgccg
     1621 acgtggaacc aggcggagcg gacgcagacg gcctcgatct tcatccagga cgcgtggacg
     1681 cgcgggcggc tgacgctgca gggcgccctg cgctacgacc gtgcctggag cttcagtccc
     1741 gccgagcaca acggaacgga gctgacctcg aagttcaacg cggcgccgat ttcgttccca
     1801 cgcacggtcg gcgtggactc gttcaacgac atcacgccgc gcgtcggcgt ggcgtacgac
     1861 gtgttcggca acgggaagac cgcgctgaag ttcaactggg gccactacct cgacgcggcc
     1921 accaacgaca gcgcgtacac ccgcaacagc ccggcgcaga acacggtgcg cacggtgacc
     1981 aaccgcggct ggaccgacca cgacggcgac aaggtcatcg attgcaaccc gatgaacttc
     2041 gccgcgcagc ccggagcgcc aggtacgggc ctggacacgt gcgccgcctt gaccggcaac
     2101 gacctgaact tcgggtcgac gagcgccaac gtgacgcagg tgaaccaggc gacgctccgc
     2161 ggctggggcg tgcgggaaaa cgactggcag tggggcctga ccgtacagca ggagatcgtc
     2221 ccccgcgtgt cgatcgacgt cggctacaac cgccggtggt ggcacggctt caccgtccag
     2281 gacaacacgg tgcgcactcc tgacgagtac accgcgtgga cgatcaacgc gccggtcgat
     2341 ccccggctcc cgggcggcgg cggctatccg atcacgatgt acaccgtgac ggcggccgcg
     2401 tcggcggaag cggcgaggaa ctacgtaacc ttcgagaccg acttcgggcc cgagcgcacc
     2461 aattactggc acggcgtcga cgtcaatgta agcgcgcgtc tccgcaacgg gctcttcctc
     2521 tccgcgggga cgagcaccgg ccgcgagatc gagaacgatt gcgcgacgca ggtcaagatc
     2581 gacagccccg acgacaggat gtgcaactcg atcgagccgt tccagacgac cgtccggggc
     2641 ctggcgacct acacgattcc gaagatcgat ctgctggtca gcggcacgct tcgctcgcag
     2701 ccgccgctgg ggcgcgtggc gacctggaac gtgccgaata cgctcgtgtc ccagccgcag
     2761 tacctcggtc gagtgccgcc ggggagcacg atcaacggca acacggctgt taccttgttc
     2821 gacaacaacg agtaccggct ctacgccgac gagcggcgga cgcaactcga catccgaatc
     2881 gcgaagatcc tgcgcttcgg tccgacgcgc gcggacgtcg gcgtcgatat cggcaacgtg
     2941 ttcaacacca actacgcgac ggcgtacgag aacacgtatc agtacagcgt gggcaacgca
     3001 gggatgggcg ggacgtggaa caacccgacc tccatctaca cgccgcgcta cttccggatc
     3061 aacacgacgg tgagcttcta
//
LOCUS       DBNGSH010000723         2213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4322806, whole genome shotgun sequence.
ACCESSION   DBNGSH010000723 DBNGSH010000000
VERSION     DBNGSH010000723.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4322806"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="AC16CY_16580"
     CDS             <1..1011
                     /locus_tag="AC16CY_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355467.1"
                     /translation="FPDNKAWRIGTPDLIVVSPPVEMKGVAPDWFGAIGTSPTGLTED
                     RYVAAVEVKEVNDLHGKTSRSSTVGGLYLFHHAAYGAITPGGGAGGAQAFPVHEVGRN
                     ADVFDAEAGRLLAANSMLTFNSVHLHANGLDTKAHLEVGFKFHPRGYKPTLNFRQLFV
                     GNGPDLDIKGLEANQRVDAYYTLPQNVKISTYEPHMHAPGVRMCLEAIWGITVQTLNC
                     SKYDHNWVRAYWYEDDAAPLLPKGTILHVVGYFDTTAANRNVADGRNWSGSGHRSVDN
                     MMINLMQAISLTDEQFEAELAKRRQRLGLKEGQTVLGCPTCGVTPSSARLPAGQAPAT
                     PN"
     gene            1021..1902
                     /locus_tag="AC16CY_16585"
     CDS             1021..1902
                     /locus_tag="AC16CY_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355468.1"
                     /translation="MRAAFAGAITLALTTLLGGSAQESFSRGQNIAPAYEGWERNADG
                     SFNLVFGYFNRNWEEEIDAPIGPANAIEPGGPDRGQPTHFQPRRNRFVFRIRVPADFG
                     TRELVWTLTTNGKTERAYATLKTDYFIDDTVIMANNGAAGMGGTNPALKGNKPPSLKI
                     EGTARTIKVGQPITLEAIAADDGIPRPRPMPALLPGIATRFTVDSATGLRLSWFVYRG
                     RGKVVFDPVQTKVWEDTRDRANSPWSYGWRTPPVPPEGKWVVRATFTEPGSYVLRCQA
                     HDGGLATTEDVTINVTN"
     gene            complement(1953..>2213)
                     /locus_tag="AC16CY_16590"
     CDS             complement(1953..>2213)
                     /locus_tag="AC16CY_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355469.1"
                     /translation="VVPVFMGMSMSGAGQHAHHTHAAGMGAGAAFFATGLHAVGYLAA
                     TAFVAVLVFEKLGVGILRRAWFNLDLLWSAALVATGTLTLVL"
BASE COUNT          434 a          738 c          720 g          321 t
ORIGIN      
        1 ttccccgaca acaaggcctg gcgcatcggc acgccggacc tgatcgtcgt ctcgccgccg
       61 gtcgagatga agggcgtcgc gcccgactgg ttcggcgcga tcgggacgtc gccgacgggc
      121 ctgaccgagg atcggtacgt ggccgccgtc gaagtcaagg aagtgaacga cctgcatggg
      181 aagaccagcc ggagcagcac cgtcggcggg ctgtacctct ttcatcacgc cgcctacggg
      241 gcgatcaccc cgggaggcgg ggccggagga gcgcaggcgt ttccggtgca cgaagtcggg
      301 cgaaacgccg acgtcttcga cgccgaggcc ggcaggctcc tggccgcgaa ctcgatgctg
      361 acgttcaact cggtacatct ccacgccaac ggccttgata caaaggcgca cctcgaggtc
      421 ggcttcaagt tccacccgcg cggatacaag ccgactctga acttcagaca gctattcgtg
      481 ggcaatggac cggacctgga tatcaagggc ctggaggcca atcaacgggt cgacgcctac
      541 tacacgcttc cccagaacgt gaagatcagc acgtatgagc ctcacatgca cgcgcccggc
      601 gttcgcatgt gcctcgaagc catctggggc atcacggtgc agaccctcaa ctgctccaag
      661 tacgatcaca actgggtgcg cgcgtattgg tacgaggacg acgccgcgcc gctgctcccg
      721 aaaggaacca tcctgcacgt cgtcggctat ttcgacacga cggcggcgaa caggaacgtc
      781 gccgacggga ggaactggtc agggtcgggt catcgctcgg tggacaacat gatgatcaac
      841 ctgatgcaag ccatctctct gaccgacgag cagttcgagg ccgagctcgc caaacgccgc
      901 cagcggcttg gcctgaaaga gggccagacc gtgctcggtt gcccgacgtg cggcgtgacg
      961 ccgtcgtcgg ccaggctgcc ggccggtcag gcgccggcga ccccgaactg aggcgcgccg
     1021 atgagagcgg ccttcgcggg tgccatcacg ctggcgttga ccactctgct cgggggcagc
     1081 gcgcaggaat cgttttcaag aggacagaac atcgctccgg cgtacgaggg ctgggagcgg
     1141 aacgccgacg gctccttcaa cctcgtgttc ggctacttca accggaactg ggaagaggag
     1201 atcgacgcac ccatcggtcc cgccaacgcg atcgagcctg gcggtcctga tcgaggccag
     1261 cccacgcatt tccagccgcg gcggaaccgg ttcgtcttcc ggatccgagt ccccgccgac
     1321 ttcgggacgc gggagctcgt gtggacgttg accacgaacg gcaagaccga gcgcgcctac
     1381 gccacgctca agaccgacta tttcatcgac gacaccgtga tcatggccaa caacggtgcg
     1441 gccgggatgg gcggcaccaa tcccgcgctc aagggcaaca agccaccctc actgaaaatc
     1501 gaaggcaccg cgaggacgat caaggtcggg cagccgatca cgctcgaagc gattgcggcg
     1561 gatgacggca tcccgaggcc gcggcccatg ccggctctcc tccccggcat cgcgacccgc
     1621 ttcacagtgg acagcgccac cgggctcagg ctgtcctggt tcgtctaccg gggccggggg
     1681 aaggtcgtgt tcgaccccgt gcagacgaag gtctgggaag acacgcggga tcgcgccaac
     1741 tccccgtggt cgtacgggtg gcgaacaccg cctgttcctc ctgagggcaa gtgggtcgtc
     1801 cgtgcgacgt ttaccgaacc cggcagctac gtgctccggt gccaggccca cgacggcggg
     1861 cttgcaacga cagaagacgt cacgatcaac gtcaccaact gagcaggggt ccgggatcag
     1921 ggggcagggg gcagggctgt ctgaggctga tctcacaaca ccagcgtgag cgtgccagtc
     1981 gccaccagcg cggcggacca gagcaaatcg aggttgaacc acgctcgccg caggatgccg
     2041 acacccagct tctcgaacac cagcaccgcc acgaacgcgg tcgcggccag gtagcccacg
     2101 gcgtgcaggc cggtggcaaa gaacgcagcg cccgcgccca tgccggcggc atgcgtgtga
     2161 tgcgcgtgct gccctgctcc ggacatcgac atgcccatga acaccggcac gac
//
LOCUS       DBNGSH010000724         2325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2455046, whole genome shotgun sequence.
ACCESSION   DBNGSH010000724 DBNGSH010000000
VERSION     DBNGSH010000724.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2455046"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1573)
                     /locus_tag="AC16CY_16595"
     CDS             complement(242..1573)
                     /locus_tag="AC16CY_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3355470.1"
                     /translation="MTNLRRLPAILIGLIALLVLTSTLIAQDSQAARYWAQWRGPHAT
                     GVSTTANPPLEWSETRNVRWKVEIPGRGSASPVVWGDRVFVLTAVPTGVTGDAQHAPR
                     GSVSPRGVHRFLVMALDRKTGKTIWERVAREQEPHEQSHVDNGTWASGSPLTDGERVY
                     AYFESFGLYAYDMNGKLLWEKDLGDKKMRNEFGEGSTPALHGNTLVVVWDHLGGESFV
                     AALDKRDGRELWRVARKEIDTWATPLVLEVNGRAQAIVPAMERVRSYDLETGAVVWES
                     DGLTMNAIPSPVHADGLVFLMSGFRGNDLKAVRVAEAKGNIDGTSAIVWSLDRDTPYV
                     PSPVLVDGVLYFLKSNSGILTAVDARTGTPHYANQRLDGVPNVFASPVAARGRVYFPG
                     REGTTLVIKSGPAFEVLAKNVLEDGFDASPALVDNEIYLRGYKYLYAIAQQ"
     gene            1683..>2325
                     /locus_tag="AC16CY_16600"
     CDS             1683..>2325
                     /locus_tag="AC16CY_16600"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3355471.1"
                     /translation="MPIIRLTLVLAFVAGASAAAADWPHWRGPNGTGATSETNLPVRW
                     SATENVAWKALIGGLGVSTPIVSGDQVVVTSQIGASERRPGNHPRLVQGGNAADAGER
                     ALGGTRRPQADDGRVHFLVESFRRADGRRLWEHRLPAEGPMPGVHDKHNLASPSPITD
                     GQMVYAWFGTGQIVALDMSGKLVWQRHLGKEISPFEVAWGHSSSPTLFGDLLIL"
BASE COUNT          378 a          781 c          781 g          385 t
ORIGIN      
        1 cactccacgc agttggcgta gaggatgggc gcgatgtcac gtgtgaacgt cggggcgccg
       61 ggcacgggct gcgccgactg cgcgcgaagg ccggcgccga cggcggcggc cagggtgatt
      121 gtgccggcaa cgaagagcac gggtgttctc atgtggacct ctgactgtga cgcggaggct
      181 gaagctgtgg cgcggaggct gaagcctccg ctctactact gcatctgaag cctccgctct
      241 actactgctg cgcgatggcg tacaggtact tgtagccccg caggtagatc tcgttgtcga
      301 cgagcgccgg cgacgcgtcg aagccgtcct ccaggacgtt cttcgccagc acctcgaagg
      361 ccgggcccga cttgatcacc agcgtcgtcc cctcgcgccc ggggaagtag acacgccctc
      421 gtgccgcgac cggcgaggca aacacgttcg gcacgccgtc gaggcgctgg ttcgcgtaat
      481 ggggcgtacc cgtcctggca tcgacggccg tgagaattcc ggaattgctt ttcaggaaat
      541 agagcacccc gtccaccagc accggcgatg gcacgtacgg cgtgtcgcga tccagcgacc
      601 agacgatcgc gctcgtcccg tcgatgttgc ccttggcctc ggccacccgg acggccttca
      661 gatcgttgcc gcggaaaccg ctcatcagga acacgagacc gtcggcgtgg accggcgacg
      721 ggatcgcgtt catcgtgaga ccgtcgctct cccacacgac cgcgccggtt tcgaggtcgt
      781 agctgcgcac gcgctccatc gcgggcacga tggcttgcgc gcggccgttc acctcgagca
      841 cgagcggcgt cgcccacgtg tcgatctcct tgcgcgcgac gcgccacaac tcgcggccat
      901 cgcgcttgtc cagcgcggcg acgaacgact cgccgccgag gtgatcccag acgacgacga
      961 gcgtgttgcc gtggagggcc ggggtcgatc cctcgccgaa ctcgttgcgc atcttcttgt
     1021 cgccgagatc cttctcccac agcagctttc cgttcatgtc gtaggcgtag aggccgaacg
     1081 actcgaagta cgcgtacacg cgctcgccgt ccgtgagcgg cgagcccgag gcccaggtgc
     1141 cgttgtccac gtgggactgc tcgtggggtt cctgctcccg ggccacgcgc tcccagatcg
     1201 tcttgcctgt cttgcggtcg agcgccatta cgagaaatcg gtgcacgcct cgcggcgaga
     1261 cgcttccgcg tggcgcgtgc tgggcatcgc cggtcacgcc cgtcggaacg gccgtgagca
     1321 cgaacacgcg gtcgccccag acgactggag aggccgagcc gcggccgggg atttccacct
     1381 tccagcgaac gttcctggtt tcgctccatt caaggggcgg gttcgccgtc gtcgagacgc
     1441 cggtggcatg cggcccgcgc cactgtgccc agtagcgcgc agcctgagag tcttgagcaa
     1501 tcagcgtcga ggtcagcacg agaagcgcta tgagtccgat aagaattgcg ggcagccgac
     1561 gaaggttcgt cacgcgggcc tccttcggtt cgaggatagc attcaactcc caactcccaa
     1621 ctgccagtcc caactctcaa cgcgttgtcg tagactcaag cttttcggac gaggacgcga
     1681 tcatgcccat cattcgactg acgctcgtgc tggccttcgt tgccggcgca tcggcggccg
     1741 cggccgactg gccgcactgg cggggaccga acgggacggg cgccacgagc gaaaccaacc
     1801 tgcccgtgcg ctggagcgca accgagaacg tggcgtggaa ggctctcatc ggcggactgg
     1861 gcgtctcgac accgattgtg tccggcgatc aggttgtggt gacgtcacag atcggcgcca
     1921 gcgaacggcg gccgggaaat cacccgcggc tcgtgcaggg cggcaacgcc gccgacgccg
     1981 gcgagcgcgc gcttggcggc acgcgacgcc cccaggcgga tgacggccgc gtccattttc
     2041 tggtggagag ctttcgccgc gcggacggcc ggcgcctgtg ggagcaccgc ctgccggccg
     2101 aggggcccat gcccggagtg cacgacaagc acaacctcgc gtcgccgagt ccgattacgg
     2161 acggtcagat ggtgtacgcc tggttcggca cgggacagat cgtcgcgctc gacatgagcg
     2221 gcaagctcgt gtggcagcgt cacctcggaa aggagatctc gcccttcgag gtggcgtggg
     2281 ggcacagcag ctcgccgacg ctcttcggag acctgctgat cctcc
//
LOCUS       DBNGSH010000725         1732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1776927, whole genome shotgun sequence.
ACCESSION   DBNGSH010000725 DBNGSH010000000
VERSION     DBNGSH010000725.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1776927"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="AC16CY_16605"
     CDS             complement(<1..1494)
                     /locus_tag="AC16CY_16605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3355472.1"
                     /translation="MNRYLLPFNHVLHKLKGSRVGRGIFLISAIALVVIVLSGGWWTA
                     RHAWAVYRLNRGVGDTVFYDADGRPWFRLDEQRQDVGYDQISKYFRDAVIAVEDHRYY
                     LHPGVDPIGLTRAAVNNARSDGRTQGGSTITQQLARTLFLSNARTYGRKAKEAALSMM
                     LELFLSKREILQLYMNRIYLGGGIYGVETMSRRAFGKPAADLSLAEAAMLAGVIRAPA
                     SYSPWNHPEDAWQRSLVVLRRMREEGKITREQELAASRERVRIRSAGPQPNARHGYAK
                     EFLRQQFRDLVGNDNPPDWKVRTTLVSEAQDAAEASVRAGLARLGIPGVQAALVAMDP
                     KSGNILALVGGSDFRKTPFNRAVRGHRQPGSAFKPFVYAAALESGLSPVSTISGLQQV
                     AIHAPEGVWIPRDDRAKTHDTMTLREALLESSNAAAVLLQQQVGARPVIQLARDLGVA
                     DQPDVPSLALGSGLVTPLDLTAAYGVFPGRGVRVRPRGVAQIEGADGS"
     gene            1497..>1732
                     /locus_tag="AC16CY_16610"
     CDS             1497..>1732
                     /locus_tag="AC16CY_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355473.1"
                     /translation="MAPSLRRLTLAVVLGSTTMAGLAQPGVQFQERDFLSRVRRLTVE
                     GRRAGEGYWSPDGRRLVFQSEREPDNPFYQIYTM"
BASE COUNT          281 a          589 c          568 g          294 t
ORIGIN      
        1 gcttccgtca gcgccctcga tctgagcgac gcctcgcggc cgcacgcgga ctccccttcc
       61 cggaaaaacg ccatacgccg ccgtgaggtc cagcggcgtc acgagcccgc tgccgagggc
      121 aagcgacggc acgtcaggct ggtcggccac gcccaggtcg cgcgcgagct gaatcaccgg
      181 ccgggcgccc acctgctgct gcagaagaac ggccgccgcg ttgctcgact cgagcagcgc
      241 ttcccggagc gtcatcgtgt cgtgtgtctt cgcgcggtcg tcgcgcggaa tccacacgcc
      301 ttcgggcgcg tggatggcga cctgctgaag cccgctgatg gtcgataccg gcgacaggcc
      361 gctctcgagc gccgccgcgt acacaaatgg cttgaacgcg gacccgggtt gccgatggcc
      421 ccggaccgcc cggttgaacg gcgtcttgcg gaagtccgag ccgccgacca gggcgagaat
      481 gttgcctgac ttcggatcca tcgccacgag cgcagcctgc acgccgggaa tgccaagcct
      541 tgcgagacca gctcgcaccg aggcctccgc ggcatcctgc gcttccgaga cgagcgtcgt
      601 gcgcactttc cagtcgggag ggttgtcgtt gccgacgaga tccctgaact gctgccgcag
      661 gaactccttc gcgtacccat ggcgcgcgtt tggctgtggg cccgcggatc ggatccgcac
      721 gcgctcgcgg cttgcggcca gctcctgctc gcgcgtgatc ttcccttctt cgcgcatgcg
      781 tcgaagcacc acgaggcttc tctgccaggc gtcttcgggg tgattccacg gcgaataaga
      841 cgccggagcg cgaatcacgc ccgcaagcat cgcggcctcc gcgagcgaca ggtcggcggc
      901 cggcttcccg aacgcgcgtc gcgacatcgt ttcgacgccg tagatgccgc cgcccagata
      961 gatccgattc atgtagagct gcaggatttc cctcttcgag aggaaaagct ccagcatcat
     1021 cgacagggcg gcttcctttg ccttgcgtcc gtaggtccgc gcgttcgaga ggaagagcgt
     1081 ccgcgccagc tgctgcgtga tggtgctgcc tccctgcgtc cgaccatcgg agcgggcgtt
     1141 gttgacggcg gcgcgcgtca gcccgatggg gtcgacgccc ggatgcaggt agtagcgatg
     1201 gtcctcgacg gcgatgacgg cgtccctgaa gtacttcgag atctggtcgt agccgacgtc
     1261 ctggcgctgc tcgtcgagcc gaaaccaggg ccggccgtcc gcatcgtaga agacggtgtc
     1321 gccgacaccc cggttgaggc ggtagacggc ccacgcgtgg cgggccgtcc accaccctcc
     1381 cgagagcacg atcacgacga gcgcaatcgc cgaaatcagg aagattccgc gcccgactcg
     1441 cgagcctttc aacttgtgaa gcacgtggtt gaatggtaat aggtatcgat tcaccgatgg
     1501 ccccatccct tagacgcctg acgctcgccg ttgttctggg ctccacgacc atggccgggc
     1561 ttgcgcagcc aggggtccaa tttcaggagc gcgacttcct gagccgcgtc agacggctga
     1621 ccgtcgaagg gcgtcgtgcc ggcgaggggt actggtcgcc cgacgggcgc cgtctggtgt
     1681 tccagagcga gcgcgagccg gacaacccgt tctatcagat ctacacgatg ga
//
LOCUS       DBNGSH010000726         2990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_928341, whole genome shotgun sequence.
ACCESSION   DBNGSH010000726 DBNGSH010000000
VERSION     DBNGSH010000726.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_928341"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="AC16CY_16615"
     CDS             complement(<1..1042)
                     /locus_tag="AC16CY_16615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3355474.1"
                     /translation="MPDISKAVRDLSASFSGRLLLPGDAGYEEARRVHNGLIDKRPAV
                     IAQCRGTADISDAVAVARTHGLRISIRGGGHNVAGRAVVDAGMMIDLSLMRSVVVDRA
                     SRTARVEGGATWKEVNRETQQFGLATTGGVVGTTGVAGLTLGGGFGWLMARHGMSLDN
                     LRSVDVVLADGTSVRASATDHADLFWAVRGGGGNFGVVSSFEFTLHDIGPTIAGGLVA
                     WPMAAARDVLRFFRDFTASIGDDVFTVCALLTGPDGATKLAAIAAAYLGPAAGAEAAL
                     APVKRFGSPVMDAMGPITYVALNGLLDGAFPKGALNYGKAPFLDRLEDRAIDAAVTAM
                     ASCPSPMSQILFE"
     gene            complement(1097..2635)
                     /locus_tag="AC16CY_16620"
     CDS             complement(1097..2635)
                     /locus_tag="AC16CY_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355475.1"
                     /translation="MNPARAVRLLVLKEARALLPIWLASAAAIVVGMADRRGPLFPFG
                     LLAFSVGAAALGAFSVGHEYAYRTLPALLAQPLPRQRFLLAKAAALAPLLASLAALAR
                     LMLVRANGGSPSGRAISSEWLEINTILVPILGFCVAPWLTLIFRHVIAGVVFTLAIPA
                     AVWLAINLVPALDGVVIDFDDVRALSIFWIMILGASAFALVDGPRRFVRLEAADGAGG
                     VSVSRTSRRNAPSAASPGRRHPLAQLVAKEIRLQRVTFLLPAFYILSWAVIVATDSTS
                     FLGPAFFAMTVAYCAVTALLVGALASAEERSLGTLAAQVLQPLAMWKQWTVKVSCALA
                     LTLVFTVVLPRALEWVHPLSQQVPVSWGMAGVFLLLATCSLYLSSVSNDGLRALLLAM
                     PFAAVAGPLFVTITEETQKAIWRPITPVLQTLSLNWTRPIPLLSGADSDWYRWSERWL
                     QVAVLCVLVALLIWMAARNHRSAEHGLGRMRRQLLWLVPCVVMGGVISGAVPPLLLWF
                     LGTH"
     gene            complement(2632..>2990)
                     /locus_tag="AC16CY_16625"
     CDS             complement(2632..>2990)
                     /locus_tag="AC16CY_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355476.1"
                     /translation="DPGRTVFVSTHLISEFEGLIDRFTIIDNGRDVLTLDADAARERY
                     QRIYARFPEEPRALDLTAAHVIRREGREVEFIARGNSMELLERVTAYAPEVVKAEALS
                     LEDIFVSTAHHTGAAA"
BASE COUNT          556 a         1002 c         1034 g          398 t
ORIGIN      
        1 gctcgaacag gatctgcgac atcggcgagg ggcacgacgc catcgcggtc acggcggcgt
       61 cgatcgcgcg gtcctccagg cgatccagga acggcgcctt cccgtagttg agcgcgccct
      121 tcggaaaggc gccatcgagc agaccgttca gggcgacgta ggtaatcggc cccatcgcgt
      181 ccatcaccgg cgatccaaag cgcttgaccg gcgccagcgc cgcctcggcg cccgcggctg
      241 gtcccaggta cgcggccgcg atcgcggcga gcttcgtcgc cccgtccgga cccgtcaaca
      301 gcgcgcacac cgtgaacacg tcgtcgccga tcgaggccgt gaagtccctg aagaacctga
      361 gcacgtcgcg cgcggcggcc atcggccacg ccacgagccc tcccgcgatg gtggggccga
      421 tgtcgtgcag cgtgaactcg aacgacgaga cgacgccgaa gttgccgccg ccgccgcgaa
      481 cggcccagaa gagatcggcg tgatcggtag cgctggcgcg gacggacgtg ccgtcggcga
      541 gcacgacgtc caccgaccgc aggttgtcga gcgacatccc atgcctggcc atgagccagc
      601 cgaagccgcc gccgagcgtg aggcccgcga cgcccgtcgt cccgaccacg cctcccgtcg
      661 tggcgaggcc gaactgctgc gtttcgcggt tgacctcctt ccaggtggcg cccccttcga
      721 cgcgcgccgt tcgcgacgcc cggtccacga cgacgctgcg catgagcgag aggtcgatca
      781 tcatcccggc gtccacgacg gcgcggcccg cgacgttgtg tccgccgccg cggatcgaga
      841 tcctcagccc gtgcgtgcgc gcgaccgcga ccgcgtcgct gatgtcggcc gttccacggc
      901 actgtgcgat cacggccggg cgcttgtcga tgaggccgtt gtgcacgcgc cgggcctcct
      961 cgtacccggc gtcgcccggc agcagcagcc ggccggaaaa ggacgccgag agatcgcgaa
     1021 ccgccttgga gatgtcgggc atgaccgggc ctccgggaag accgacgatt ctacaacgcc
     1081 ggccgcgggg ctgaggtcag tgcgtaccga ggaaccagag gagcagcggc ggtacggccc
     1141 cggagatcac tccgcccatg acgacgcagg gcacgagcca caggagctga cgccgcatgc
     1201 gcccgaggcc gtgttcggcc gatcggtggt ttctggcagc catccagatc aggagtgcga
     1261 cgagaacaca caggacggcc acctgcagcc atctctccga ccagcgatac cagtcggaat
     1321 ctgcccctga gagcagaggg atgggtcgcg tccagttcag gctgagcgtt tgaaggacgg
     1381 gagtaatggg acgccagatc gccttttggg tctcctcggt gatcgtcacg aacagcgggc
     1441 cagccaccgc cgcaaagggc atcgcaagca gcagcgcgcg caacccgtcg ttgctgacgg
     1501 aggaaaggta taggctgcag gtcgcgagca atagaaatac gccggccatt ccccaagaca
     1561 cgggcacctg ctggctcaaa ggatggaccc attccagtgc cctgggcagc acgaccgtga
     1621 agacaagggt gagcgcgagc gcgcatgaga ctttcacggt ccactgtttc cacatcgcga
     1681 gcggctgcag cacctgggcg gccagggttc caagagaacg ctcctccgcg ctcgccagcg
     1741 cgccgacgag cagcgcagtc acggcgcagt acgcaacggt catcgcgaag aaagcgggac
     1801 ccaggaacga ggtcgagtcg gtggccacga tgacggccca ggacaggatg tagaacgcag
     1861 gcagcaggaa cgtcacgcgc tgcagcctga tctccttggc gacgagttgc gcgagcgggt
     1921 ggcgacggcc tggcgacgcg gccgacgggg catttcgtcg gctggttctg gacaccgaga
     1981 ccccgccggc gccgtcggcc gcctcgagcc ggacgaacct ccgcggtcca tcgaccagcg
     2041 cgaaagcgct ggcgcccagg atcatgatcc agaagatgct cagagcacga acgtcgtcga
     2101 aatcgatgac gacgccatcg agcgcgggca ccaggttgat cgcaagccac accgcagcgg
     2161 ggatggcgag cgtgaacacc acgccggcga tcacgtgtcg gaagatcaag gtgagccacg
     2221 gagcgacgca gaagcccagg atgggcacga gaatcgtgtt gatttcgagc cattcactgg
     2281 agatcgccct gccggatggg gagcctccat tggctcgcac gagcatcagc cgcgcaagcg
     2341 cggcgaggct ggcaagcaac ggcgcgaggg ccgcggcctt cgccagcagg aagcgttgac
     2401 gagggagagg ctgtgcgaga agcgccggga gagtgcgata cgcgtactca tggcccaccg
     2461 agaacgcacc gagcgcggcg gcgccaaccg agaatgcaag caaaccgaat ggaaagagcg
     2521 ggcctcgtct gtcggccatg ccgacgacga tcgcggccgc cgacgcaagc cagatgggca
     2581 gcagcgcgcg cgcttccttc agcacgagca gcctgacggc ccgcgccggg ttcacgctgc
     2641 cgctcccgtg tggtgcgccg tggagacgaa gatgtcctcg agcgagagcg cttcggcctt
     2701 caccacttcc ggcgcgtagg ccgtgacgcg ctcaagcagt tccatggagt tgccgcgcgc
     2761 gatgaactcg acttcgcgcc cctcgcgccg gatgacgtgc gcggcggtca ggtcgagcgc
     2821 cctgggctcc tccgggaagc gcgcgtagat tcgctgatag cgctcgcggg ccgcgtcggc
     2881 gtcgagcgtg aggacgtcgc ggccgttgtc gatgatggtg aaccgatcga tgagcccctc
     2941 gaactccgag atgaggtgcg tcgagacgaa caccgtgcgg cccggatcgg
//
LOCUS       DBNGSH010000727        12241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1098098, whole genome shotgun sequence.
ACCESSION   DBNGSH010000727 DBNGSH010000000
VERSION     DBNGSH010000727.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 12241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12241
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1098098"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2337
                     /locus_tag="AC16CY_16630"
     CDS             <1..2337
                     /locus_tag="AC16CY_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3355477.1"
                     /translation="PNCSQDLDTILYTAVSSYVSDSGERQKMLSLLPEISKRTRDLMR
                     SAISAKDEAPRPQEFIRQVTHEVNGFSPQAKEEFFNVIDKVPMAYQKANAIFATPEKK
                     NPGSGGIFSIFVSDLCKGCAACVTACGEHEALRMVQETEEVNAEHESGTAFLNLLPDT
                     SQKYLGLYNDVRPQDSKTATLRNMLMVRKNYDALVSGDGACAGCGEKSILRAVAAVTE
                     AYMRPIYHAKADRLRAKAGELDKDGVAKLAALKERNPQEYELFRQAVAHMLMGLGGED
                     DKDTQKRIAAHGPISDKDIVDAMTAVMRQEAFNHKNLQPVDGRLANGMSVMAMAAHTG
                     CNTVYGSTPPNNPHPYPWMNSLFQDGITVGWLIGESFIVDHGRRSVIPERLADALMRR
                     EADVINPREFYEYVHFTDATMTDQEILELPKVWVVGGDGGMGDIGYQNMSKVVLQNRP
                     NVKAVMLDTQVYSNTGGQNSDSTPMLGGNDMNVFGAATQGKNVEKKTVAETFLAGHGS
                     PFVAQVSIANAPKLYRAILDGIEYRGTAFLQCFTTCQPEHGVADDLALTQAQRVRDSR
                     GAPEFVFNPRLGETYQEALDLKGNPTLDMDWYETKFKTSNTPYRYTVAHWCATEARFR
                     NHLRKVKKDDLARLIPLENMLVRITQQDVVYRRYLVSGHRSYVPDFGVYITIQGANGD
                     VEYRSLSRQLVLFCVERRKAWRMLQSKAGIENREYKAQRALLGDVDAGKISKEHLFAH
                     AEQLLKERLPAPAAHKPAEPKTPPPAAAKPAAATATVN"
     gene            2387..2809
                     /locus_tag="AC16CY_16635"
     CDS             2387..2809
                     /locus_tag="AC16CY_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3355478.1"
                     /translation="MKRSASAVWRGGLKDGKGTVSTESGVLSTVPYNFRMRFENEKGT
                     NPEELVAAAHAACFSMALSLFLGEAGMTAESIETTATVTLEQVGGGFSVTSSHLKTAV
                     KIPNADKAAFQKAAEGAKSGCPISKLLNTSITLDAKLN"
     gene            2893..4734
                     /locus_tag="AC16CY_16640"
     CDS             2893..4734
                     /locus_tag="AC16CY_16640"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3355479.1"
                     /translation="MIGRTVSHYKILSPLGAGGMGVVYLAEDTRLGRQVALKFLPISW
                     SQDTKALDRFRVEARAASSLSHPSICAIFDIGQDGDTPFIVMEALKGTTLRDRILKGP
                     LKVADVLEIGMQLADALEAAHAQGIVHRDIKPANIFLGERNRVKVLDFGLAKLTGSSP
                     APSGESDTTSSNERVKSNQMTQPGTALGTVSYMSPEQARGELVDSRTDLFSLGAVLYE
                     MVTGVQAFGGSTTAVVYDAILNRTPRLIASLNPLVPARLETVIATALEKDRDLRYQHA
                     SDLQAELKRIRRDLDTQSLAGSQTVVLPSRPSAPAAPVTAAAPSQARANWPARIAILL
                     AGLAAAAAAVLFFRGQPAGERAAAPASDAPAISSAQPAGTTVSPPPPAATIPAPASKP
                     EAAPALPAPTPQPRPRATAPSETSPDARAPLPSPAVTPARQAQPVDVEAAPSPPPTAT
                     PSPTSPPPVAPAAPEEVKQQPPRTAAPAEQTVAPPAPVPAQAAPPPRPATPEPAPPAA
                     ETDDAAIRRVIRVYEQAIEAKDIALYRSVRTNLTRAAETVLTNSFRQIDSQEIDIKIE
                     SLRIEGRTASARLVRRDTLVTGGRREVRNSTQTMRFDKTDAGWLIAE"
     gene            4766..6091
                     /locus_tag="AC16CY_16645"
     CDS             4766..6091
                     /locus_tag="AC16CY_16645"
                     /inference="COORDINATES: protein motif:HMM:NF024946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355480.1"
                     /translation="MRVTTVAAVITLLAAVEPARAQEPYNVTVNVPELSRIFTELYGP
                     DGLVVNSLATLTGGVSHSAHFNSAFETEFSQFGTALTSQIVSLPLPAPASGFTYEFDQ
                     ALGVFTRSTSTFGPILAERADTIGARRVSIGFAYQRLAFDAIEGMDLQSIPAVFTHDN
                     AELRGGREDVITTVNSIDSEVTRSAGFITYGVTDRLDVSVVIPYISADIVVTSDATIR
                     RIGTTIPEIHFFRAADDSIGDRRLFTAFGHASGPGDITVRLKQVVKKGQVHGFALGLD
                     LRLPTGDERNLLGTGAAGVQPFGAWSADYGVFSPHVNVGYQWNGSSILGGDLDSGVAG
                     DLPDVGVYALGAVVAVHPRVTVAFDVVGRYIIDTPIVRPEDFHALDGRSVFPNIAFEN
                     GSINELSSAVGLKINLAGRLLLNTNLLASLNSGGLRDKISPLVGIEYAF"
     gene            complement(6199..7290)
                     /locus_tag="AC16CY_16650"
     CDS             complement(6199..7290)
                     /locus_tag="AC16CY_16650"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3355481.1"
                     /translation="MVTSSGEEVIGANAKYECCKFRGSVSALNAATGEVRWKSFTIPE
                     TPVPVRKNKQGVQLWGPSGAGVWSSPAVDLKRGAVYVTTGDSYSDPAASTSDAFVAFD
                     IKTGKLLWSRQTTAGDAFTVDCGLPEAARTNCPEANGPDFDFGSSPILIDLPGGGRAL
                     VAGQKSGVVHGIDPDRNGAILWQTRVGKGTALGGVQWGSAFDGRRVYVALSDVQPEPV
                     PAGTSGGQPTAFGVPMRLNSRAGGGLFALNPATGAIVWNTPHPGCGERTGCSPAQSAA
                     VTAIPGVVFSGGLDGHLRAYASETGAIVWDADTAQPYETVNGVKANGGSLDGPGPVVV
                     GGMLFVNSGYAFVGGMPGNVLLAFTVDGR"
     gene            complement(7291..8106)
                     /locus_tag="AC16CY_16655"
     CDS             complement(7291..8106)
                     /locus_tag="AC16CY_16655"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3355482.1"
                     /translation="MDRRHAMRLASALVFAVGVPAFGQAQNAPNPEAEALYKSRCASC
                     HDAGVARAANRETLRSLSPDAIRLALTSGSMRTQGADLTTAQVETLARLLGGAAAATA
                     PRRADNACPAGQAAFTNPLEQPNWNGWGANLSQHRFQPAGMAQLPAGDVPRLKLKWAF
                     GFPGVNRAPAQPTVAGGRLFVGSAANTVYSLNANTGCQYWALKTDAPVRTAISIGRIP
                     GAGAGRWLAYFGDQGANAYAVDALTGEVVWKVKVENFFGATITGAPTLADETL"
     gene            complement(8158..8754)
                     /locus_tag="AC16CY_16660"
     CDS             complement(8158..8754)
                     /locus_tag="AC16CY_16660"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3355483.1"
                     /translation="MAHELPKLPYDFAALEPHIDSQTMQIHHGKHHQAYVTNLNAALD
                     KHPELHGKSLDELCRGVNAVPEDIRTAVRNNGGGHWNHGRFWTWMAPNAGGAPTGKIA
                     SAINASFGSFDAFKDQWGKAGAGRFGSGWVWLLNDGGKLSITSSPNQDNPLMDGKTAI
                     LGLDVWEHAYYLKYQNRRPDYITAWWNVVNWAEVNKAL"
     gene            complement(8893..10029)
                     /gene="nadA"
                     /locus_tag="AC16CY_16665"
     CDS             complement(8893..10029)
                     /gene="nadA"
                     /locus_tag="AC16CY_16665"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR3355484.1"
                     /translation="MASLIPLLTDFEKQHLSERQALPEKYLGLNDEEMDVRIAAARRA
                     LGARLVILGHHYQRDEVIRFADYTGDSFRLARQIANRPDADFIVFCGVHFMAESADVL
                     CAPHQRVILPDLAAGCSMADMAAPGQLETCWEELTQMGTRGVVPVTYINSAASIKSFV
                     GERGGTVCTSSNAMATLKWAFARGERILFLPDQHLGRNTAYKMGIPLEDMVVWDPDEI
                     CGGLDPGQVDRARMILWKGHCSVHTRFSAKQIQGVRQQYPGVRVIVHPEVPWDVVQAA
                     DDSGSTEYIIRTVTNSPEGSIWAVGTEVHLVSRLAQQLAPHRTVVSLDQFGCLCSTMF
                     RVSPNHLLWILEELLEGRVHNQIVVPDEQKHWNRVALDRMLSIS"
     gene            complement(10199..11329)
                     /gene="mqnE"
                     /locus_tag="AC16CY_16670"
     CDS             complement(10199..11329)
                     /gene="mqnE"
                     /locus_tag="AC16CY_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HIR3355485.1"
                     /translation="MKSRLLAAGLADIDAKLSRGERLTLEDGVRLFETPDLLAVGWLA
                     NREREKRHGARTYFNYNLRLEPTNVCEASCLFCSFARLKPGMPEAYTMSLEEAWDKLR
                     QRSNQPLTELHIVNGLNPDLPFDYYVELLRGFKRIRPGIHLKCFTAVEIAFFADLYGM
                     TDAQVLRELMDAGLDSLPGGGAEIFAERVRQKICSDKCGTERYLEIHRTAHRLGMRSN
                     VTMLYGHIETMAERVDHMMRARALQDETAGFQAFIPLAFHPDNNQMRKLPAPSGTDTL
                     RVHAVSRLMLDNIPHIKAFWIATGLELAQAALWFGVDDLDGTVQEEKIYHMAGSMTPE
                     GMSTAALGRLVRTAGRQPLERDTLYNIINSQPPTPNSQEVLT"
     regulatory      11441..11575
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11669..>12241
                     /locus_tag="AC16CY_16675"
     CDS             11669..>12241
                     /locus_tag="AC16CY_16675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355486.1"
                     /translation="MSISATRLSYTYRRRTSSEWVHALSDVSLDVARGSLTGLIGPNG
                     CGKTTLLKLLAGILAPDSGTVALDGHSLATMSQRQIARRIAVVPQETHPAFDYTSLEM
                     TLMGRHPHLGPFELEGPSDVAIAREALRATGTDHLADRLYMTLSGGEKQRVVIASALA
                     QSPEVLLLDEPTASLDLGYQLEIASLLRQLN"
BASE COUNT         2142 a         4095 c         3953 g         2051 t
ORIGIN      
        1 ccgaactgct cgcaggatct cgacacgatt ctgtacaccg cggtgagcag ctacgtcagc
       61 gacagcggcg agcggcagaa gatgctgagc ctgctgccgg agatcagcaa gcggacgcgg
      121 gacctgatgc gcagcgcgat ctccgccaag gacgaggctc cgcgtccgca ggagttcatc
      181 cgccaggtca cgcacgaggt caacggcttc tcgccgcaag ccaaggaaga gttcttcaac
      241 gtcatcgaca aggtgccgat ggcgtaccag aaggcgaacg cgatcttcgc aacgccggag
      301 aagaagaacc cgggctcggg cgggatcttc tcgatcttcg tctcggatct ctgcaagggg
      361 tgcgcggcct gcgtcaccgc ctgcggcgag cacgaggcgc tgcggatggt gcaggagacc
      421 gaggaggtca acgccgagca cgaaagcggc acggccttcc tcaacctgct gcccgacacg
      481 tcgcagaagt acctcgggct ctacaacgac gtgcgcccgc aggattcgaa gaccgccacg
      541 ctccgcaaca tgctgatggt gcggaagaac tacgacgcgc tcgtctcggg cgacggcgcc
      601 tgcgccgggt gcggggaaaa gagcatcctg cgcgccgtgg cggccgtgac cgaggcgtac
      661 atgcggccga tctatcacgc caaggccgac cgcctgcgag ccaaggcggg cgaactcgac
      721 aaggacggcg tcgcgaagct ggcggcgctg aaggagcgca atccgcagga gtacgagctc
      781 ttccggcagg ccgtggctca catgctgatg ggtctcggcg gcgaggacga caaggacacg
      841 cagaagcgga tcgccgcgca cggcccgatc tccgacaagg acatcgtcga tgccatgacc
      901 gcggtgatgc ggcaggaagc gttcaaccac aagaacctgc agccggtgga tggacggctg
      961 gccaacggga tgtccgtgat ggccatggcg gcgcacaccg ggtgcaacac cgtgtacggc
     1021 tcgacgcccc cgaacaaccc acatccgtac ccgtggatga actcgctctt ccaggacggc
     1081 atcaccgtcg gctggctcat cggcgaaagc ttcatcgtcg atcacgggcg ccggtccgtg
     1141 atccccgagc ggctggccga cgcgctcatg cgccgtgagg ccgacgtcat caacccgcgc
     1201 gagttctatg agtacgtgca cttcaccgac gcgacgatga ccgaccagga gattctcgag
     1261 ctgccgaagg tgtgggtggt gggcggcgac ggaggaatgg gcgacatcgg gtaccagaac
     1321 atgtcgaaag tggtcctgca gaaccggccg aacgtgaagg ccgtcatgct ggacacgcag
     1381 gtctactcga atacgggagg ccagaactcc gattcgacgc cgatgctcgg cggcaacgac
     1441 atgaacgtgt tcggcgccgc gacgcagggc aagaacgttg agaagaagac cgtggccgaa
     1501 acgttcctgg ccggccacgg ctcgccattc gtggcccagg tctcgattgc caacgctccg
     1561 aagctgtacc gggcgatcct cgacggcatc gagtaccgcg gcacggcgtt cctccaatgc
     1621 ttcacgacct gccagcccga acacggcgtg gccgatgatc tggcgctgac gcaggcgcag
     1681 cgcgtgcgcg attcgcgcgg cgcccccgag ttcgtgttca acccgcggct tggcgagacg
     1741 taccaggaag cgttggatct caaggggaat ccaacgctcg acatggactg gtacgagacg
     1801 aagttcaaga cgagcaacac gccctaccgc tacaccgtgg cccactggtg cgccaccgag
     1861 gcgcggttcc gcaaccacct gaggaaggtc aagaaggacg atctggccag gctgatcccg
     1921 ctcgagaaca tgctcgtccg catcacgcag caggacgtcg tctaccgccg gtacctcgtg
     1981 tccggtcacc gctcctacgt gcccgacttc ggcgtctaca tcacgattca gggagccaac
     2041 ggcgacgtcg agtaccggtc tctctcgcgt cagctcgtgc tcttctgcgt ggagcggcgg
     2101 aaggcctggc gcatgctcca gagcaaggcc gggatcgaga atcgggaata caaggctcag
     2161 cgggcgctgc tcggcgacgt ggacgcgggc aagatctcga aggagcacct cttcgcgcac
     2221 gccgagcaac tgttgaagga gcgcctgccc gcgcccgcgg cgcacaagcc ggccgaacca
     2281 aagactcccc cgccggctgc ggcgaagcca gcagcggcga ccgcaacagt gaactgagac
     2341 caaccgtaga ggcgggtctt gagacccgcc tttcgaggac accatcatga aacgaagcgc
     2401 gagcgccgtc tggcgcggtg gattgaagga cggaaaaggg acggtgtcca ccgagagcgg
     2461 cgtgctctcc acggtgccct acaactttcg catgcggttc gaaaacgaaa agggcaccaa
     2521 ccccgaggaa ctggtggccg ccgctcacgc ggcgtgcttc tcgatggccc tgtcgctctt
     2581 ccttggcgag gcggggatga cggcggagag catcgagaca acggccacgg tcacgctgga
     2641 gcaggtcggc ggcggattct cggtgacgtc gagccacctc aagacggccg tcaagatccc
     2701 caacgccgac aaggccgcgt ttcagaaagc ggctgagggg gccaagagcg gctgtccgat
     2761 ctcgaagctg ctgaacacgt ccatcacgct cgacgcgaag ctgaactgac gcgggtgatc
     2821 ctttgacagg gccttgcgcc ttcagccatg cttgctgatt gctgaccctg gcgtcttgct
     2881 gaccatcgag cagtgatcgg ccgaaccgtt tcgcactaca agatcctcag cccgctcggc
     2941 gccggcggga tgggcgttgt ctatctcgcc gaagacacgc ggctggggcg ccaggtcgcg
     3001 ctcaagttcc tccccattag ctggtcgcag gacacgaagg cgctcgaccg gttccgcgtc
     3061 gaggcgcgcg ccgcgagctc gctcagccac ccttccatct gcgcgatctt cgacatcggc
     3121 caggacggcg acacgccttt catcgtcatg gaggcgctca aaggtacgac gctccgcgac
     3181 cgaatcctca agggaccgct gaaggtggcc gacgtcctcg agatcggcat gcagctcgcc
     3241 gacgcgctgg aggccgccca cgcgcagggc atcgtgcatc gggacatcaa gccggcgaac
     3301 atcttccttg gcgagcgcaa tcgcgtgaag gtgctcgatt tcggcctcgc gaagctcacc
     3361 ggatcgtctc cggcgccgtc gggcgagtcc gataccacgt cgtcgaacga gcgtgtgaag
     3421 agcaaccaga tgacgcagcc cggtacggcg ctcggcacgg tgtcctatat gtcgcccgag
     3481 caggcgcgag gggagctggt cgattcgcgc acggatctgt tctcgctggg cgccgtgctg
     3541 tacgaaatgg tgaccggcgt tcaggcgttc ggaggcagca cgacggccgt cgtctacgac
     3601 gcgattctca accgtacgcc ccgcctgatt gcgtccctga atccgctcgt cccggcgcgt
     3661 ctcgagaccg tcatcgcgac cgctctcgaa aaggatcgcg acctgcggta ccagcatgcc
     3721 tcggatctgc aggcggagct gaagaggatt cgtcgcgacc tcgacacgca gagcctggcc
     3781 gggagccaga cggtcgtcct gccgtcgagg ccgagtgccc cggcggcgcc agtcaccgct
     3841 gcggccccgt cccaggccag ggccaattgg ccagccagga tcgcgatcct cctcgccggc
     3901 ctcgcggccg cggccgcggc cgtgctcttc ttccgaggcc agcccgccgg cgagcgcgcg
     3961 gctgcgccgg cgagcgatgc acccgcgatt tcatccgcgc agccggccgg cacgacggtc
     4021 agtccccctc cgccggcggc aacgattccg gcgccggcat cgaagccgga agcagccccg
     4081 gcgctgccgg ccccgacacc gcaaccgagg cctcgcgcca cagcgccgtc cgagacatca
     4141 cccgatgcac gcgcgccgct gccgtcacca gccgtcacac cggccaggca ggcacagccg
     4201 gtggacgtcg aggcagcgcc atcgccaccg ccgacggcca caccgtctcc aacgtcgccg
     4261 ccacctgttg cgcctgctgc tcccgaagag gtaaagcagc agcccccacg gactgcggcg
     4321 cccgcagagc agaccgttgc gccgccagct cctgttccgg cacaagcagc cccgccgccg
     4381 cgccccgcca cgcccgagcc cgcgccgcca gcggccgaga ccgacgacgc cgcgatccgt
     4441 cgagtgattc gcgtctacga gcaggcgatc gaggccaagg acatcgcgct gtaccgttcg
     4501 gtgcggacga atctgacccg tgcggcggaa accgtgctga cgaacagctt caggcagata
     4561 gactcccagg agatcgacat caagatcgag agcctgagga tcgaaggccg caccgcgtcg
     4621 gctcgactcg ttcggcggga tacgctcgtg acgggcgggc ggcgtgaagt caggaacagc
     4681 acgcagacca tgcggttcga caagaccgac gccggctggc tcattgccga atagcccctg
     4741 tttcaaccca tcctggagga catcgatgcg cgtcacgaca gttgccgcgg tgatcacgct
     4801 tctggccgcc gttgaacccg cacgggcgca ggagccgtac aacgtcaccg tgaacgtccc
     4861 cgaactgtcg cgaatcttca ccgagctgta cgggccggac ggtctggtcg tgaacagcct
     4921 ggcgacgctc acgggcggcg tctcgcactc ggctcatttc aacagcgcct tcgagaccga
     4981 gttctcccag ttcggcaccg cgctcaccag ccagatcgtc tcgttgccac tgcccgctcc
     5041 ggcgtccggc tttacctatg agttcgacca ggcgctcggc gtgttcacgc ggtccacgtc
     5101 tacctttggt ccgattctcg ccgagcgcgc ggacacgatt ggtgcgcgtc gtgtgtcgat
     5161 cgggttcgcc tatcagcggc tggccttcga cgcgatcgag gggatggatc tgcagtcgat
     5221 accggccgtc ttcacgcacg acaacgccga actgcgcggc ggccgcgagg atgtcatcac
     5281 gacggtgaat tcgatcgact cggaagtcac ccggtcggcg ggattcatca cctacggcgt
     5341 gaccgacagg ctcgatgtgt cggtggtcat cccgtacatc tccgccgata tcgtcgtgac
     5401 ctccgacgcg acgattcgtc gcatcggcac caccatccca gagattcatt tcttccgcgc
     5461 cgcagacgac agcatcggcg accggcgctt gttcacggcg ttcggccacg cgagcgggcc
     5521 cggcgacatc acggtccggc tgaaacaggt ggtcaagaag ggtcaggtgc acggcttcgc
     5581 gctcgggctc gacctgcggc ttccgacagg cgacgagcgg aatctcctgg gcactggtgc
     5641 ggccggcgtt caaccattcg gcgcctggtc ggcggactac ggcgtctttt caccccacgt
     5701 caacgtcgga taccagtgga acggatcgag catccttggc ggcgatctcg attcgggtgt
     5761 ggcgggcgat cttccggatg tcggcgtcta cgcgcttggc gcggtcgtcg cggtgcaccc
     5821 tcgagtgacc gtggcgttcg acgtcgttgg acggtacatc atcgacacac ccatcgtgcg
     5881 tcccgaagac ttccacgcgc tcgacggccg atccgtcttt cccaacatcg cgttcgaaaa
     5941 cggatccatc aacgaactca gcagcgccgt cgggctcaag atcaacctcg cgggccggct
     6001 gctcttgaac acgaacctgc tggccagcct caactcgggc ggcctccgcg acaagatctc
     6061 gccgctcgtc ggcatcgagt acgcgttcta gagtttcggc gagtctgcct tcgactacgc
     6121 tcaggccagg aaagacccgc cgctactggt atcaggatcg ccggtgtacg tagtgtccgg
     6181 ctttagccgg acccggacct atcgtccgtc gacagtgaac gccagcagga cgttgccggg
     6241 catgccgccc acgaaggcgt agcccgagtt cacgaacagc atgccgccga cgacgacggg
     6301 acccgggccg tcgagcgatc cgccgttggc cttgacgccg ttgacggtct cgtacggctg
     6361 cgccgtgtcc gcgtcccaga cgatggcgcc cgtctcgctg gcgtacgcgc gcaggtggcc
     6421 gtcgagccct cccgagaaga ccacgcctgg aatcgccgtg accgccgccg actgcgcggg
     6481 gctgcagccg gtccgctccc cgcagccggg gtgcggcgtg ttccagacga tggcgcccgt
     6541 cgcaggattg agggcgaaca ggccgccgcc cgcacgactg ttcaagcgca tcggcacacc
     6601 gaacgccgtt ggctgccccc ccgacgttcc cgccgggacc ggctccggtt gcacgtccga
     6661 gagcgccacg tacacgcgcc gaccgtcgaa agcggaaccc cattgcacgc cgccaagcgc
     6721 ggtccccttg ccgacgcgcg tctgccacag gatggcgccg ttgcgatcgg gatcgatgcc
     6781 gtgcacgaca ccggacttct gtccggcgac gagcgctctg cctccgcccg gaagatcgat
     6841 caggatcggc gacgatccga aatcgaagtc ggggccgttg gcttctgggc aattggtccg
     6901 cgccgcttcc ggaaggccac agtccacggt gaacgcatcg ccagccgtcg tctgccgcga
     6961 ccagaggagc ttgcccgtct tgatgtcgaa ggcaacgaag gcgtcggacg tgctggccgc
     7021 ggggtcggag taactgtccc cggtagtcac gtacacggcg ccgcgcttga gatcgacggc
     7081 gggcgacgac cagacgcccg cgcccgaggg tccccagagt tgaacgccct gcttgttctt
     7141 gcgcaccggc accggcgttt cgggaatggt aaagctcttc cagcgcacct cgcccgtggc
     7201 cgcgttcagc gcggacacgc tgccgcggaa cttgcagcat tcgtacttgg cgttggctcc
     7261 gattacctcc tcgcccgaag aggtcaccac ctacagcgtc tcgtcggcga gcgtcggtgc
     7321 tccggtgatg gtcgctccga agaagttttc gactttgacc ttccacacga cttccccggt
     7381 aagcgcgtcc acggcgtacg cgttcgcgcc ctggtcgccg aaataggcca gccaccgtcc
     7441 ggcaccggcg ccggggattc ggccgatgct gatcgcggtt cgcacgggag cgtcggtctt
     7501 gagcgcccag tactggcagc cggtgttcgc gttcagcgaa tacaccgtgt tggccgcgct
     7561 cccgacgaac aggcgtccgc ccgcgacagt tggctgcgcc ggggcccggt tgacgcccgg
     7621 gaatccgaat gcccacttga gtttcagccg cggcacgtcg ccggccggca actgcgccat
     7681 ccctgccggc tgaaaccgat gctgcgacag gttggcgccc cacccgttcc agttcggctg
     7741 ctcgagcggg ttggtgaatg ccgcctgccc tgcggggcac gcgttgtccg cgcgccgcgg
     7801 cgccgtcgcc gcagccgcac ccccgagcaa ccgggccagc gtttctacct gggctgtggt
     7861 gagatcggcg ccctgcgttc gcatgctgcc gctcgtcaac gcgagccgga tcgcgtctgg
     7921 cgagaggctt ctcaatgtct cgcggttggc cgcgcgtgcc actcccgcgt cgtggcacga
     7981 ggcgcagcgg ctcttgtaga gggcctctgc ttcaggattc ggcgcgttct gagcctgacc
     8041 gaatgctgga acgccgaccg cgaagaccag agcgctcgcg aggcgcatgg cgtgacgtct
     8101 gtccatggct tcgaagacgg agatccgccg agcgaggttc cagcgcgttg acgctgctca
     8161 gagcgccttg ttgacttcgg cccagttcac gacgttccac cacgccgtga tgtaatccgg
     8221 gcggcggttc tggtatttca ggtagtaggc gtgctcccac acgtcgaggc cgaggatggc
     8281 cgtcttgccg tccatgagcg ggttgtcctg gttcggcgag ctcgtgatcg acagctttcc
     8341 gccgtcgttg agcagccaca cccagccgct tccgaatcgt cccgcacccg ccttgcccca
     8401 ttgatccttg aacgcgtcga agctgccgaa cgacgcgttg atcgcgctgg cgattttgcc
     8461 cgtcggcgcg ccgccggcgt tgggggccat ccaggtccag aaccggccgt ggttccagtg
     8521 cccgccgccg ttgttgcgca cggcagtccg gatgtcctct gggacggcgt tcacgccgcg
     8581 gcacagctcg tcgaggctct tgccgtgcag ctcgggatgc ttgtcgagcg cggcattcag
     8641 gttggtgacg tatgcctgat gatgtttccc gtgatgaatc tgcatcgtct gcgagtcgat
     8701 gtgcggctcg agcgcggcaa agtcgtacgg gagcttgggc agttcgtgcg ccattcgtcc
     8761 tcctgagaaa tggggtcggg agtcatttat ggaaaatggg gtcgggagtc atttatcgag
     8821 gacagcgtgc gtgtgtcgtt gagcactacc ctcgataaat gactcccgac cccattttcc
     8881 aggagcccca tgttatgaga tggacagcat ccggtccagc gccacgcgat tccagtgctt
     8941 ctgctcgtcc ggcaccacaa tctgattgtg gacgcgccct tcgagaagct cctccagaat
     9001 ccacagcaga tggttgggag agacgcggaa catggtggag cagaggcagc cgaactgatc
     9061 cagcgacacg accgtgcggt gcggcgcaag ctgctgcgcc aggcggctca cgaggtggac
     9121 ttctgtaccc acggcccaga tcgatccctc ggggctgttg gtgacggtcc tgatgatgta
     9181 ctcggtgctt ccgctgtcgt cagccgcctg cacgacgtcc cacggcactt ccgggtgaac
     9241 gatgactcgc acgcccgggt actgctgccg gaccccctgg atctgttttg cgctgaacct
     9301 cgtgtggacc gagcagtgtc ccttccacag gatcattcgt gcgcgatcga cctggccggg
     9361 atcgagcccg ccgcagatct cgtcggggtc ccacacgacc atgtcctcga gcgggattcc
     9421 catcttgtac gccgtgttgc ggcccagatg ctgatccggg aggaacagga tccgctcgcc
     9481 tcgcgcaaac gcccacttga gcgtcgccat cgcattggag gaggtgcaca ccgtgccgcc
     9541 ccgctcgccg acaaaagact taatcgaggc ggcggagttg atgtaggtga cgggcaccac
     9601 tcccctggtg cccatctggg tcagctcttc ccagcaggtt tcgagctggc cgggcgcggc
     9661 catgtcggcc atcgagcagc cggcagcgag gtcgggcagg ataacgcgct ggtgcggcgc
     9721 gcagagcacg tccgcgctct ctgccatgaa gtggacgccg cagaacacga tgaaatcggc
     9781 gtcgggccgg ttcgcgatct gccgcgcgag acgaaacgag tcgcccgtgt agtctgcgaa
     9841 gcggatgacc tcgtcgcgct gatagtgatg gcccaggatg acgaggcggg cgccgagcgc
     9901 gcgcctggcg gcggcgatgc gcacgtccat ctcctcgtcg ttgaggccaa ggtatttctc
     9961 gggaagggcc tgccgctcgg acaggtgttg cttctcgaag tcggtgagca gcggaatcag
    10021 cgatgccatc ggcgcacgcg cgcttgtgaa acttttcgca aagtgcggac gcgaaaaagc
    10081 ccaaacccca cgtaccgggc catcgcccca tgggatttgg acctactgga attatccgtc
    10141 cgaggggggg ccgagatcaa cttccaactc ccaaatccca acccccaaac ggcgctcgct
    10201 atgtgagcac ttcttgggag ttgggagttg gaggttggga attgatgatg ttgtagagcg
    10261 tgtcccgctc cagcggctgg cgccccgccg tccgcaccag acggcccagc gctgccgtgc
    10321 tcatgccttc cggcgtcatc gatccggcca tgtgataaat cttctcctcc tgcacggtcc
    10381 cgtcgaggtc atcaacgccg aaccacaacg ccgcctgcgc cagctccagc cccgtcgcga
    10441 tccagaacgc cttgatgtgc gggatgttgt cgagcatcag ccgcgacacg gcgtgtacgc
    10501 gcagcgtgtc ggtcccgctc ggcgcgggaa gcttgcgcat ctggttgttg tcggggtgaa
    10561 acgccagcgg gatgaacgcc tggaaccctg cggtttcgtc ctggagcgcg cgcgcgcgca
    10621 tcatgtgatc gacccgctcg gccatcgttt cgatgtggcc gtacagcatc gtcacgttcg
    10681 agcgcatgcc gagccggtgc gcggtgcggt gaatctcgag gtagcgctcg gtcccacact
    10741 tgtcactgca gatcttctgt cgcacgcgtt cggcaaaaat ctcggcgccg ccgcccggca
    10801 gcgaatcgag gcccgcgtcc atcaactcgc gcagcacctg cgcgtccgtc atgccgtaca
    10861 gatcggcgaa gaacgcaatc tcgaccgccg tgaagcactt gagatgaatc cctggccgga
    10921 ttcgcttgaa gccccgcagc agctcgacgt agtagtcgaa cggcagatcc ggattcaggc
    10981 cgttgacgat gtgaagctcg gtgagcggct gattggagcg ctgacgcagc ttgtcccacg
    11041 cctcttcgag cgacatcgtg taggcctcgg gcatgccggg cttgagccgc gcgaaagaac
    11101 agaagaggca gctggcctcg cagacgttgg tcggctcgag gcgcaggttg tagttgaagt
    11161 acgtgcgggc cccgtgacgc ttttcgcgct cgcggttggc cagccatccg accgccagca
    11221 gatccggcgt ctcgaacaga cgaacgccgt cctcgagcgt gagccgttcg ccccggctca
    11281 acttggcgtc gatgtcggcc agtccggccg ccagcaggcg tgatttcacg tcagggctct
    11341 ccgaaattct agcgtgctat actcggcgtc agaaattggg gtcgggagtg attttcgaga
    11401 aaatgactcc cgaccccatt acaaactcgt caggtgagtg agggaagtgg gtggaacacc
    11461 cacgcggtcc cgccactgta agcggaaagg gtcgatcgca cgcgtcactg tgtccggtcc
    11521 actggacatg ggaagacagg tcgaaccgtt cttgattccg cgagccagga tacctcgcat
    11581 ctgacacctg tcccgtgcac ctcgacgccg ctcgaacccg ggcggcggcc gatgggtgct
    11641 ccggcgcgaa ggacgccggg aggagacgat gtccatttcc gccactcgac tttcctacac
    11701 gtaccgaagg cgcacgtcgt ccgagtgggt ccacgcgctg tctgacgtca gccttgatgt
    11761 cgctcgcggt tcgctgacag gcctcatcgg gcccaacggc tgcggcaaga caacgctgct
    11821 caagctgctc gccggcattc tcgcgcccga cagcggaacc gtcgcgctgg acgggcactc
    11881 cctggccacg atgtcgcagc gccagatcgc gcgacgcatt gccgtggtcc cccaggagac
    11941 acacccggcg ttcgactaca cgtcgctcga gatgacgctc atggggcgtc acccgcatct
    12001 cggcccgttc gagctcgagg ggccctccga cgtggccatc gcgcgggagg cgctgcgggc
    12061 cacggggacc gatcatctgg cggaccgcct gtacatgacg ctcagcggcg gcgagaagca
    12121 gcgcgtcgtc atcgccagcg cccttgcgca gtcgccggag gtgctgctcc tcgacgaacc
    12181 caccgcctcg ctcgatctcg ggtatcaact ggaaatcgcg tcgctgctgc ggcagttgaa
    12241 c
//
LOCUS       DBNGSH010000728         5982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2456133, whole genome shotgun sequence.
ACCESSION   DBNGSH010000728 DBNGSH010000000
VERSION     DBNGSH010000728.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2456133"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1044)
                     /locus_tag="AC16CY_16680"
     CDS             complement(100..1044)
                     /locus_tag="AC16CY_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355487.1"
                     /translation="MNLAHLHLLMNHVPTVGSVAALGVLLLAYVRRNEHLKHVGLELL
                     FVIAVLTLPVYVSGVAAQFQLQEVPEVSRDAVMVHQDAALIGFAVIEFAGFVAWLALW
                     QWRRHGRAGQGLVPVATVLLLLALVLMGRAATLGGEIRHPEIRAADAAGEPAGDPKRF
                     MTSAIGQVAVYSTWVWPAAEAIHFLGLALSFGVLLAVNLRILGVMKRVSFGDVHRLLP
                     WGMLGLGANLITGMFFFVGQPGQYATSDPFLWKVVFLVIAGANFLYLTVFGKAWPHRE
                     GAVFDAGVADKAMAVLSLSAWLAVLYAGRMLPFLGKSF"
     gene            complement(1050..1520)
                     /locus_tag="AC16CY_16685"
     CDS             complement(1050..1520)
                     /locus_tag="AC16CY_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HIR3355488.1"
                     /translation="MLSWLESTSLAVWLQESPSIWSLPTVLTLHTAGMAVLVGASWVL
                     DLRLLGIGRSIPLSAYRWVFPTLAIGLFVNLVTGVLLFVKNATTLGTALPFFVKMLLV
                     VASVATLLPLRSLVIRGGSEQGEADGRARLLAIASILAWTGAVTAGRLLAYLIV"
     gene            complement(1630..2535)
                     /locus_tag="AC16CY_16690"
     CDS             complement(1630..2535)
                     /locus_tag="AC16CY_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355489.1"
                     /translation="MSKQRIIVLACTAAVLFVVGVSLAAQGGQGGRGGGAPAVDPNAP
                     TPRLPSGKPDLTGSWQGGGLITGSGVQGTGWQVGNMFRRCTPFQPKTCMEWTNQSIDW
                     VFMSTSRLGQNQPMYKPEYWNKVIELDQWTNKYDPVGSCLPLGVPRQGPPARIFHTDT
                     DITMIYRGGVDGAGGYAEFRMVPIDGKPHNPQRANNYTYMGYTVGRWEGDTLVLDSIG
                     FSDETWLARGGWFHSDQMRVVEKFTRAGNQITYDVTVEDPVVLLKPWVMPTVIMRNTG
                     GNTIIGERGSCTDNEQREASSQYRH"
     gene            complement(2546..3571)
                     /locus_tag="AC16CY_16695"
     CDS             complement(2546..3571)
                     /locus_tag="AC16CY_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355490.1"
                     /translation="MSERRTLATGVLVLLVAMSQVEYGGAQAQGGGAPQGQGAAPQGR
                     GAGAPQGRGAAAPQGRGRGDAGAFTPEPGAKDLKSVLFNWTWHMGMLRSGAESELIKT
                     MDYHGAGGSIQVNGQPCTLTRYHLQANYLLPGYRTDIACTLKSKQTYSVVENMSGDYA
                     WDDDIVGAELIPGKGKSTPRPETLTERRIRLWASPHGAPKAALAAAAGMHPLQSFGQN
                     PATLLDRQAAAGVKGTTTLTWEGEKAVLTFPVPDVPGATATATLVNYLAERVVVTNGK
                     DTTEFVYSKWADFNNPLFKIDALIPTTTVERKNGTAARNVTSKVTEIGQIYVIVPVPD
                     SIQKAGK"
     gene            complement(3591..3989)
                     /locus_tag="AC16CY_16700"
     CDS             complement(3591..3989)
                     /locus_tag="AC16CY_16700"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355491.1"
                     /translation="MKRLAVGLAILSGFLMVGAGLQAHHAVAGVYDLNKEIVLEGRLK
                     KLNFTNPHASIELSVPDKKVKGKVTDWILTTASVQTLTREGINKTSMKPGEVLKVTVL
                     PARNGNPAGFIRNLQLGDRNILLYFGDGTD"
     gene            complement(4079..5062)
                     /locus_tag="AC16CY_16705"
     CDS             complement(4079..5062)
                     /locus_tag="AC16CY_16705"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3355492.1"
                     /translation="MPVSINLPVLNADDVLAGLRGLRTKQPVKFSAFYSSQLGGIVTD
                     PALMVIPFDDHMVHRGHGIFDTAGLVNGRIYDLEAHLDRFLGSAERSKLRLAGSREDM
                     REIIVKTTAASGLRDGAIRYWLSSGPGSLELSPAQGAEPGFFVMIFGGLSYSARWYTE
                     GLRVMTTTYPIKPPLYAITKATNYLPNVLMQMEAKEAGLDNGVFIDESGFVGESSNMN
                     LAFVTDDGVLRHPKFERVLAGCTSRRVLELAQGLVGHVLTGVEVCDIPVAAARAAREM
                     VLFGSSVKVAPIVEWDGRPIGDGKPGPAAKALLELLEKDMREGDRLIDVPY"
     gene            complement(5218..>5982)
                     /locus_tag="AC16CY_16710"
     CDS             complement(5218..>5982)
                     /locus_tag="AC16CY_16710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3355493.1"
                     /translation="DLYEPRAVALISEDLARREWGGAAAALGKRLRGGSGLNIWREVV
                     GIVGNVHNRGVSEPAGGTVYLPVLTDLAVARNVSFVLRSERAGTAGLLADVQRAVWAV
                     DPELPLADVRTMGDYYGASLSRTSFTLVMLAVAGVMALLLGVIGIYGLMSYVVSQRTR
                     EVGIRMALGAQRSEIGGMFMRQGLALTLVGVALGLAGAVALARWMSVLLFGVSPFDLA
                     TYVSVSAALIAAAGLASYLPTRRATRFDPIAALRVE"
BASE COUNT         1078 a         2025 c         1870 g         1009 t
ORIGIN      
        1 ctagccgccg gtgcgatcga gaaggtagcc aagggcgccc acgatcacca ggacgacgag
       61 gaccgttgcg gttacggcaa acgccaccat cgcgcagcct cagaaggact tgcccaggaa
      121 aggaagcatt cgacccgcgt acagcaccgc gagccacgcc gagagggaca ggaccgccat
      181 cgccttatcg gccacgcccg cgtcgaacac ggcgccttcg cgatgcggcc aagccttgcc
      241 gaataccgtc aggtacagga agttcgcacc cgcgatcaca aggaaaacga ccttccacaa
      301 gaacgggtcg ctcgtcgcgt actgcccggg ctgcccaacg aagaagaaca tcccggtaat
      361 gagattggcg ccaaggccaa gcatgcccca gggcaacagc cggtgcacgt ccccgaaaga
      421 gacccgcttc atcacgccga ggatccgcag gttcaccgcc agcagcacgc cgaacgagag
      481 ggcgagaccc aggaagtgaa tcgcttcggc cgccggccag acccacgtgc tgtacaccgc
      541 cacctgaccg atcgccgagg tcatgaatcg tttcggatcg ccggcaggct ccccggccgc
      601 gtctgcggca cgtatctccg ggtgccggat ctcgccgcca agcgtcgccg cgcgacccat
      661 gagcacgagg gccaggagca ggagcaccgt cgcgacgggc acgagccctt gtcccgcgcg
      721 gccgtgccgc ctccactgcc acaaggcaag ccacgcgacg aatcccgcga actcgattac
      781 cgcgaacccg atgagcgccg cgtcctggtg gaccatcacg gcgtccctcg agacctccgg
      841 cacctcctgc agctggaatt gcgccgcaac gccgctgacg tagacaggaa gcgtcaggac
      901 cgcgatcacg aacagcagtt ccagccctac gtgcttcagg tgctcgttac gccggacgta
      961 cgccaggagc aacacgccga gtgccgcgac cgatccgaca gtcggcacgt ggttcatcag
     1021 gaggtgcaga tgggcgagat tcacgggggc tacacgatga gataggcgag aagacggccc
     1081 gcggtcacgg cgccagtcca cgcgaggatc gaggcgatgg ccagcaggcg cgcgcgcccg
     1141 tcggcctcgc cctgttccga tccgccgcgg atcaccagcg agcgaagcgg cagcaacgtg
     1201 gcgacgctcg cgacaaccag cagcatcttg acgaagaatg gcaacgccgt tcccagggtc
     1261 gtcgcgttct tgacgaagag caggaccccg gtcacgagat tgacgaacag accgatggcg
     1321 agcgtcggaa agacccagcg gtaggcggag agaggaatgc tccggccgat gccgagcagg
     1381 cgtaagtcga gcacccagct ggcgccgacc aggaccgcca ttcccgccgt gtgaagcgtc
     1441 aggaccgtcg gcagcgacca gatcgacggc gactcctgca gccagacggc cagcgaggtc
     1501 gattcaagcc aggacagcac gactagtggt atgtccttcg cggattacta ccggccgctc
     1561 cgtcgtcgcg gccggaagat cgcggtcgaa gctcgttgtt cgcggtactc ttccagttga
     1621 tgcagtccac tagtgccggt actggctgct ggcttctctt tgctcgttat ccgtgcagct
     1681 tccgcgctcg ccgatgatcg tgttgccgcc cgtattccgc atgatgaccg tcggcatgac
     1741 ccacggtttc agaagcacca ccgggtcctc gacggtgacg tcgtaggtaa tctggttccc
     1801 ggccctggtg aacttctcca cgacccgcat ctgatcggag tggaaccacc cgccgcgcgc
     1861 cagccaggtt tcgtcggaga agccgatgga gtcgaggacg agcgtgtcgc cctcccacct
     1921 gccgaccgtg tagcccatgt aggtgtagtt gttggcccgc tgcggattgt gcggcttccc
     1981 gtcgatcggg accatgcgga actccgcgta cccgcccgcg ccgtcaacac cgccacggta
     2041 gatcatcgtg atgtcggtat cggtgtggaa gattcttgcc ggcggtccct gcctcggaac
     2101 accgagcggg aggcaggacc cgaccgggtc gtacttgttc gtccactggt cgagctcgat
     2161 gaccttgttc cagtactccg gcttgtacat cggctgattc tggccgagcc gcgacgtgga
     2221 catgaacacc cagtcgatgc tctggttcgt ccactccatg cacgtctttg gctggaacgg
     2281 cgtgcaccgc cggaacatgt tgcccacctg ccaacccgta ccctggacgc cggagccggt
     2341 gatgaggccg cctccctgcc aggagcccgt caggtcaggt ttcccgctgg gcagtctggg
     2401 cgtcggcgcg ttcggatcca ccgctggagc cccgccaccg cggccaccct gcccaccctg
     2461 cgcggccaga gacacaccga cgacgaacag gactgccgcc gtgcacgcga gaacgatgat
     2521 tcgctgcttg ctcatcgccg aatcctcact ttccagcctt ctggatactg tccggaaccg
     2581 gcacgatgac gtatatctgg ccgatctcgg tcactttcga ggtcacgttg cgagcggccg
     2641 tgccgttctt gcgctcgacg gtcgtggttg gtattaaagc gtcgatcttg aacaagggat
     2701 tgttgaagtc cgcccacttg ctgtacacga actcggtcgt gtccttcccg ttcgtcacta
     2761 cgacccgctc ggcgaggtag ttgacgagcg tcgcggttgc cgtcgcgcca ggaacatccg
     2821 ggacggggaa cgtgagcacc gccttctccc cttcccacgt cagcgttgtc gtccccttca
     2881 ctccggcggc ggcctgccga tcgagaaggg tggccgggtt ctggccaaag gactgcaacg
     2941 ggtgcatccc tgcggcggca gcgagcgcgg ccttgggagc tccgtgcggg ctggcccaga
     3001 gacgaatccg gcgctcggtc agcgtttcag ggcgtggtgt tgacttgccc ttgccgggaa
     3061 tcagttccgc gcccacgatg tcgtcgtccc aggcgtagtc cccactcatg ttctcgacga
     3121 ccgagtaggt ctgcttgctc ttgagcgtgc acgcgatgtc cgtccggtat cccgggagca
     3181 ggtagttcgc ctgaaggtga tacctcgtca gcgtgcaggg ctggccgttc acctggatcg
     3241 aaccgcccgc gccgtggtag tccatcgtct tgatcagttc ggactcggcg ccgctccgca
     3301 gcatccccat gtgccaggtc cagttgaaga ggaccgactt caagtccttg gctccgggct
     3361 ctggcgtaaa cgcgcccgca tcacctcggc cacgcccctg aggcgctgcg gctccacggc
     3421 cttgcggcgc tccggcgccc cggccctgcg gcgcggcacc ctggccctgc ggtgctccgc
     3481 ctccctgggc ctgcgcgccg ccgtactcca cctggctcat cgcgacaaga agaacgagca
     3541 cacctgtcgc gagagttcgt cgttcagaca tcgccaaccc ccgggttctg ttagtccgtt
     3601 ccgtcgccga agtagagcag gatgttgcgg tccccgagct ggagattcct gatgaagccc
     3661 gcggggttcc cgttcctcgc cggaaggacc gtcactttga ggacctcgcc aggcttcatc
     3721 gaggtcttgt tgattccctc gcgtgtcagc gtctgcacgg aggccgtggt gagaatccag
     3781 tcggtcacct tgcccttgac cttcttgtcc ggaactgaga gctcgatgct cgcgtgcgga
     3841 tttgtaaaat tcaacttctt gagccggcct tccaggacga tctccttatt caggtcgtac
     3901 acgcccgcga cggcatgatg ggcctggagc ccggcaccga ccatcaggaa tccggacagt
     3961 atggccaacc caacggcgag tcttttcata gcagctgctc catttccgtg atttcacgcg
     4021 gatcgtagca catcggcagc catccgatca gcctggaggg actgcgatgg accgcccctc
     4081 aatagggcac gtcgatgagc cgatctcctt cccgcatgtc cttctcgagc aactccagca
     4141 gtgccttggc ggcggggcct ggcttgccgt cgccaatcgg ccggccatcc cactcgacga
     4201 tcggcgcgac cttcacggag ctgccgaaca ggaccatctc gcgcgcggcg cgcgcggcag
     4261 ccacggggat gtcgcacacc tcgacaccgg tcaggacgtg acccacgagt ccctgcgcga
     4321 gctccagtac tcgtcgcgac gtgcatcccg ccagcacgcg ctcgaacttc ggatgacgaa
     4381 gcactccgtc gtctgtgacg aatgcgaggt tcatgttgga gctctcgccg acgaacccgg
     4441 actcgtcgat gaacacaccg ttgtccagcc cggcctcctt cgcttccatc tgcatgagga
     4501 cgttcggcag atagttggtt gccttggtga tggcgtacag cggcggcttg attggatagg
     4561 tcgtcgtcat cacgcgcagg ccttcggtgt accagcgagc cgagtaggac aatcctccga
     4621 agatcatcac gaagaagcct ggctcggctc cttgcgccgg actgagctcc aggctgccag
     4681 gtcccgaaga cagccagtac cggatggcac catcgcgcag gccgctggca gcggtcgtct
     4741 tgacgatgat ctcgcgcatg tcttcgcgcg aaccggcgag cctgagcttg gaacgttcgg
     4801 cggaaccgag gaatcgatcg agatgcgcct cgagatcgta gatgcggccg ttcacgagcc
     4861 ctgcggtatc gaagatgcca tggccgcggt ggaccatgtg atcgtcgaag gggatcacca
     4921 tcaaggcagg gtcggtgacg atgcccccga gctggctgga atagaaggcg gagaacttga
     4981 cgggttgttt cgtccgaagc ccgcgcaagc ccgcgagcac atcgtcggcg ttcagcaccg
     5041 gaagattgat cgagacaggc atgccttcac tgtattctag tggaccgtat caactggttc
     5101 ggtagtgggc acaaccagcc accgagcgcg acctggcggc cgcgacaacg gagcggccgc
     5161 tactgattcg ggagttacgc tccactagtc agcccctccg cggcgtgctg tcgttcgcta
     5221 ctcgactcgc agcgcggcga ttggatcgaa acgcgtcgcg cggcgagtgg gcaggtagct
     5281 ggccagaccg gcggcggcga tcagggccgc ggagacggag acgtaggtcg ccaggtcgaa
     5341 cgggctgacg ccgaacagca gcaccgacat ccagcgcgcg agcgcgacgg cacccgccag
     5401 acccagggcc acgccgacca gcgtcagcgc caggccttgc cgcatgaaca tcccgccgat
     5461 ctcgctgcgc tgcgccccga gcgccatccg gatgcccacc tcgcgcgtgc gctgggagac
     5521 gacgtaggac atcaggccgt agatgccgat cacgccgagc agcagcgcca tgacgccggc
     5581 gacggccagc atcacgagcg tgaacgacgt gcgcgacagc gaggcgccgt agtagtcccc
     5641 catcgtgcgc acgtcggcca gcggcagctc cggatcgacc gcccagacgg cccgctgcac
     5701 gtcggccagc agacccgccg tgccggcgcg ctcgctgcgc aacacgaaag acacgttacg
     5761 ggcaaccgcg aggtccgtca gaacggggag atacaccgtc ccgccggctg gctcgctcac
     5821 gccacggttg tgcacgttgc ccacgatgcc gaccacctcg cgccagatgt tcaggcccga
     5881 tccgccacgc aggcgtttgc cgagcgccgc tgcggcacct ccccattccc gccgtgcgag
     5941 atcctccgag atcagcgcga cggcgcgtgg ctcgtagaga tc
//
LOCUS       DBNGSH010000729         2553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4324059, whole genome shotgun sequence.
ACCESSION   DBNGSH010000729 DBNGSH010000000
VERSION     DBNGSH010000729.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4324059"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="AC16CY_16715"
     CDS             complement(<1..392)
                     /locus_tag="AC16CY_16715"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355494.1"
                     /translation="MTRYLVGLAAAATLFVAAQAYAHHSFAATYLEDQSVQIEGELVQ
                     FLFRNPHSFVHVVVKEKDGSMVRYAVEWGGAGALGGQGVTRETLKAGDHVIITGSPGR
                     NPKDHRVRMVTLHRPKDGFGWGKRPGEVV"
     gene            complement(551..2311)
                     /locus_tag="AC16CY_16720"
     CDS             complement(551..2311)
                     /locus_tag="AC16CY_16720"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR3355495.1"
                     /translation="MAFVVPAFVAAQRDEPTFPARAVGVSSAGLRQTFTNYWEWRLAE
                     SPELATRVGRTEFNDRWRDWSAPARARARAARAEFLQQVQYVAVGNLTAAEHLSADLL
                     QYELRSAIESEPYPGLVQPVSQVDGLHNEVFRTIEQMPAATVLDYENIVARLRGLPAY
                     VDQSIAMMREQLTAGLAQPAVTVSLMIDQVAAQGSAPPDQSPLLAAFAAFPAGVPQAD
                     RNRLLAEARSAYSQQFVPSWKRLETFLQDTYLKAARAEVGIGTLPNGDRAYASLIHLY
                     TTTRTDAREIHRLGLQEVSRIENEMASVVREAGFSGSVAEFERRLRSEPSTAFSSQEE
                     MLQYAREVLARVEPQLSRVVTRMPRVNVGVRPIPADREASTPSSYTAGTADGSRQAWF
                     NMNTYRPREQRKYPIEALVLHETVPGHHLQVGLARELEGLPEFRKVFAASAFSEGWAL
                     YAESLGGDLGVYRDPATRFGQLASEQFRAVRLVVDTGIHAMGWSRDRARDYFAEHVPA
                     QSIAEVDRYIARPAQALAYKLGELKIRELRRKAERELGAAFDLRGFHDAVLRNGALPL
                     EFLDEQIDLYISVAKSNRGK"
BASE COUNT          418 a          879 c          820 g          436 t
ORIGIN      
        1 acgacttcgc cgggacgctt gccccagccg aatccgtcct tcggccgatg cagcgtcacc
       61 atgcgcacgc gatgatcctt cgggttgcgg ccgggagaac cggtgatgat cacgtggtcg
      121 cccgccttga gcgtttctct ggtcacgccc tgtccgccca gcgcgccggc gccgccccac
      181 tcgacggcgt aacgcaccat ggagccgtct ttctccttca ccaccacgtg cacgaaggaa
      241 tggggattcc tgaagaggaa ttgcaccagc tcgccctcga tctgcacgct ctgatcttcg
      301 agatacgtgg ccgcgaacga gtgatgagcg tatgcctgtg ccgcgacgaa cagcgtcgct
      361 gcggcagcca gaccaaccag atagcgcgtc atatcgtggt tcctccctga cagcatcgaa
      421 cttctcacca aatcgtagcc ggaagacaag agaaatggtc ctgaagagtg cctgaattct
      481 ttgtcagaaa ccccggccgt acggcgcaga gaatagcacg tttcgccgac cgttcgccga
      541 tggttccgcg tcattttccg cgattcgact tcgccaccga gatgtacagg tcgatctgct
      601 cgtccaggaa ctcgagcggc agcgccccgt tccgcaggac ggcgtcgtgg aaaccgcgga
      661 ggtcgaaggc cgcgcccagc tcgcgctccg ctttccggcg cagctccctg atcttcagct
      721 ccccgagctt gtaggcgagt gcctgcgccg gccgcgcgat atatcgatcg acctcggcga
      781 tcgattgcgc cggcacgtgt tccgcgaagt agtcgcgcgc gcggtcgcgc gaccagccca
      841 tcgcatgtat cccggtgtcc accacgagcc gcaccgcgcg gaactgctcg ctggccagct
      901 gtccgaaccg cgtggccgga tcgcgataca cgccgagatc gccgccgagc gactcggcgt
      961 acagcgccca gccctccgaa aacgccgatg cggcgaacac cttccggaac tcgggcagac
     1021 cctcgagctc gcgggccagg cccacctgga ggtggtgacc gggaaccgtc tcgtgcaaca
     1081 ccaacgcctc gatggggtac ttcctttgct cccggggccg gtacgtgttc atgttgaacc
     1141 aggcctgccg gctgccgtcg gctgtccccg ccgtatagct cgacggggtg gacgcctcgc
     1201 ggtcggcggg aatcgggcgg actccgacgt tcacgcgggg catccgcgtg acaacgcgcg
     1261 acagctgcgg ctccacgcga gcgagcacct cgcgtgcgta ttgcagcatc tcctcctggc
     1321 tcgagaaggc cgtggacggt tcgctccgca gccggcgctc gaactccgcc acgcttccgc
     1381 tgaagccggc ctctcgcacg acgctggcca tctcgttttc gatccgtgag acttcctgca
     1441 ggccgagccg gtgtatctcg cgcgcgtcgg ttcgcgtggt cgtgtacagg tggatcaggc
     1501 tcgcgtacgc ccggtcgccg tttggcagcg tcccgatccc gacctccgcc ctcgcggcct
     1561 tcaggtacgt gtcctgtaga aacgtttcga gccgcttcca gctcggtacg aactgctgtg
     1621 agtacgccga tcgggcttcc gccagcaggc ggttccgatc ggcctgagga actccggcag
     1681 gaaatgccgc gaacgccgcc agaagcggcg actggtccgg cggtgcgctt ccctgcgccg
     1741 ccacctgatc gatcatgagc gagacggtga cggccggctg cgccaggccg gcggtgagct
     1801 gctcgcgcat catcgcgatc gactggtcca cgtaggccgg aagcccgcgc aggcgcgcga
     1861 cgatgttctc gtagtcgagt accgtggccg cgggcatctg ttcgatcgtc ctgaacacct
     1921 cgttgtgcag gccatcgacc tgcgacaccg gctgaacgag acccgggtac ggctcggact
     1981 cgatggccga gcgcagctcg tactggagca ggtccgcgct gaggtgctcg gctgccgtca
     2041 ggttgccgac ggcgacgtac tggacctgct ggaggaactc tgcccgagcg gcgcgcgcgc
     2101 gggcccgggc gggcgccgac cagtcacgcc agcgatcgtt gaactcggtg cggccgaccc
     2161 gcgtggccag ctccggcgat tccgcgagcc gccattccca gtagttggta aacgtctgtc
     2221 gcaggcccgc cgacgacacg cccacggcgc gcgcgggaaa cgtcggctcg tcccgctgcg
     2281 cggccacgaa ggccggaacc acgaaggcca cgaagatcgc gaaggacgcg aagagccatc
     2341 caagattccg gtttcgtgca cttcgtgatc ttcgtgcctt cgtggttacc acctcgggct
     2401 ccggggtcac gccttgggaa gacggaacgc gatcagctcg ccaggaaacc cggggccgct
     2461 gatggcgacc acgatgtact gctggccgtt gaggaggtag gtcatcggcg acccggtctg
     2521 cggagccggc atgtagaccg agcccacttc ctg
//
LOCUS       DBNGSH010000730         2926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1947230, whole genome shotgun sequence.
ACCESSION   DBNGSH010000730 DBNGSH010000000
VERSION     DBNGSH010000730.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1947230"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="AC16CY_16725"
     CDS             complement(<1..547)
                     /locus_tag="AC16CY_16725"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF025065.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3355496.1"
                     /translation="MSYTRKEFEFPRLERVISGPDTVDTLADEIDRQRITRALVVTGK
                     TLGSSRLLERITHPLGDRCAAVFTGARQHVPSSTVTELIALIERHEVDGVVSLGGGSP
                     IDTVKAAIHSLLTAGGASTDAVPRHVAIPTTLSAGEFTSVAGVTDERTRIKHALRDPG
                     IAPRAVVSDPTLTLATPSWLWA"
     gene            complement(591..1661)
                     /locus_tag="AC16CY_16730"
     CDS             complement(591..1661)
                     /locus_tag="AC16CY_16730"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3355497.1"
                     /translation="MSALVKGIHHITLCPGPAQEDVNFCTGVLGQRLVKQNVLLDGSI
                     PIYHFYYGNRDADVGSIATSFPYSRKQGRRGSGQVSAMGYSVPAGTIQFWMEHFARHG
                     VEHSGVQERFAEKFILARHPAGLLFEIVEAGDARRPWTTNEISEDVATRGFFGAVMSV
                     RDVEQQERFLVEAMGFLKIGVDGPYHRFEAPVCAPGRVIDLHHEPDRAPGSWGFGAGI
                     FHHIAFEVDDDEALLAQKALYEELGYTDASDVKDRYYFHSMYVRSPGGILVECTASAQ
                     GGFYRDESPEDLGTRLNLPPWYESQRAEILSMLEPVVLPDANRPRREPTPVPELEPVA
                     MAAAARGVVPSRTKADFARHRR"
     gene            2009..2902
                     /locus_tag="AC16CY_16735"
     CDS             2009..2902
                     /locus_tag="AC16CY_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator
                     domain-containing protein"
                     /protein_id="HIR3355498.1"
                     /translation="MAKNSRAEFVASLERGLRVLSTFSRAHPQLTLSEVAALTDLTPA
                     TARRSLHTLEMLGYVGRTGRRFLLRPKVLAIGTGYLSAINAEVVLQPFLQEVVNEVGG
                     SSSVTVLDEVDIVYIAHASLNRMIRLTAGAGSRYPVYPTSMGRVLMAFQPESAIEAYF
                     QRATLRKLTEHTETDQGLLRRILKDVRTKGYAAIQDELDYGIVSVALPIFGPHGRIVA
                     AANCSDVTNRLDRDTMIKKRLPVLRQAVRRIEGMLTQHPELTNSVESLASDYARSNGV
                     ARVGDATDRNERRVPLRASRR"
BASE COUNT          533 a          953 c          965 g          475 t
ORIGIN      
        1 ccgcccagag ccacgacggc gtcgccagcg tgagggtggg atcgctgacg acggcacgcg
       61 gcgcgattcc cgggtcgcgc agggcatgct tgatgcgcgt tcgctcgtcg gtcaccccgg
      121 caaccgacgt gaactcgccc gccgacagcg tcgttggaat ggccacgtgc cgcgggacgg
      181 cgtctgttga cgcacccccc gcggtgagca gcgagtggat cgcagccttc accgtatcga
      241 tcgggcttcc gccgcccaag ctgacgacgc catcgacctc gtgccgttcg atcagcgcga
      301 tcagctccgt gacggtactg gaagggacat gctgccgggc gccggtgaac acggccgcgc
      361 accgatcgcc aagagggtgg gtgatgcgct cgaggagcct cgacgatccg agtgtcttcc
      421 ccgtgacgac cagcgcgcgc gtgatgcgct ggcgatcgat ctcatcggcc agcgtgtcga
      481 cggtatcggg gccggagatg acccgctcga gtcgcggaaa ctcgaattcc tttcgcgtgt
      541 aactcaaaac tcaagactca agactcaaga ctcaaaactc agaggcgacg tcaacgccgg
      601 tgtctggcga agtctgcttt cgtccgtgac ggcacgacgc cccgcgcggc ggcggccatg
      661 gccaccggct ccagttctgg cacaggcgtt ggctcgcgcc tgggacgatt ggcgtcgggc
      721 aggacgaccg gctcgagcat cgacaggatc tcggcgcgtt gcgattcgta ccagggaggc
      781 agattgagcc tggttcccaa atcctcgggc gattcgtccc gataaaaccc gccctgcgcg
      841 ctggccgtgc actcgacgag gattccgccc ggcgaccgga cgtacatcga gtgaaagtag
      901 tagcgatcct tcacgtccga cgcgtcggta tacccgagct cttcgtacag cgccttctgt
      961 gccagcagcg cctcgtcgtc gtcaacctcg aaggcgatgt gatggaagat gccggccccg
     1021 aagccccagc ttcccggggc gcgatcgggt tcgtgatgca ggtcgatgac gcgcccgggc
     1081 gcgcacacgg gagcctcgaa gcggtgatac ggtccgtcga cgccgatctt gaggaagccc
     1141 atggcctcga cgaggaaccg ttcctgttgc tcgacgtcgc gcaccgacat cacggcgccg
     1201 aagaagccgc gcgtggccac gtcctcgctg atctcgttcg tcgtccaggg acgccgggca
     1261 tcgccggcct cgacgatctc gaacagcagc cccgccggat gccgtgcgag gatgaacttc
     1321 tccgcgaatc gctcctggac gccggagtgc tcgacgccgt gccgcgcgaa atgctccatc
     1381 cagaactgaa tcgtgcctgc cgggaccgag tagcccatgg ccgatacctg cccggagccc
     1441 cggcggccct gcttgcggct gtacggaaac gacgtggcaa tcgacccgac gtcggcgtct
     1501 cgattgccgt aatagaagtg gtagatcgga atgctcccgt cgagcagcac gttctgcttg
     1561 acgaggcgct gcccgagcac gccggtgcag aagttcacat cctcctgtgc aggccccggg
     1621 cacagggtga tgtgatggat gcctttgacg agcgcgctca tcggctggac cctgaactcg
     1681 catcataagc gaccgcgcgc gacgtagtcg agatcgcggg tatggaaagg gaaacggaag
     1741 accgaagaaa aaggggcacg ccacccgcgg aggagggcgg cgcgccccgc cggacgatcc
     1801 cgaaccagac gccgggacgg catcgggttc tacgacacgt caggcatcac gtggacctgc
     1861 agcgagcggc ccaattgtcg cgctcagcgc ccgtgctttc gctgtgcgaa acctttttcc
     1921 tgatgcggag aaaaacgtta cacgcgggat ccactgctgt caagaactga cagatcactc
     1981 cttttcgcta taatctcgat cgcttcgcat ggcgaaaaat agccgggcag aattcgtcgc
     2041 gtccctcgag cgcgggttgc gcgtcctctc gacgttctcg cgcgcacatc cgcaactgac
     2101 cttgagcgag gtggccgcgc tcaccgacct gacacccgcg acggcccggc gatccctgca
     2161 cacgctcgag atgctgggat acgtcggacg caccggccgc cggttcctcc tgcggccgaa
     2221 ggtcctcgcg atcgggacgg ggtacttgag cgccatcaac gcggaagtcg tcctccagcc
     2281 gtttctccag gaggtcgtca acgaagtggg cggcagctcc tcggtcacgg ttctcgacga
     2341 ggtggacatc gtctacatcg cgcatgcctc gctgaaccgc atgatcaggc tgacggcggg
     2401 ggcgggctcg cggtacccgg tgtatccaac gtcgatgggg cgggtgctga tggcgtttca
     2461 gcccgagtcg gcaatcgaag cgtatttcca gcgcgccacg ctccggaagc tgaccgagca
     2521 caccgagacc gaccagggct tgctccggcg gatcctcaag gacgtgcgaa cgaagggcta
     2581 cgcggcgata caggacgagc tcgactacgg catcgtgtcc gtggcgctgc cgatcttcgg
     2641 cccccacgga cggatcgtgg cggcggcaaa ctgctcggat gtgaccaatc gcctggatcg
     2701 cgacacgatg atcaagaagc ggctgccggt gctgcggcag gccgtgcggc ggatcgaagg
     2761 gatgctgaca cagcatcccg agctgaccaa ctcggtcgaa tcgctcgcct cggactacgc
     2821 gcgcagcaac ggcgtcgcgc gcgtcgggga cgcgacggat cggaacgagc gccgcgtccc
     2881 gctgcgggcg tcgcggcgat aaaaggggtc gggagtcaga aaaggg
//
LOCUS       DBNGSH010000731         3696 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_929310, whole genome shotgun sequence.
ACCESSION   DBNGSH010000731 DBNGSH010000000
VERSION     DBNGSH010000731.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_929310"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="AC16CY_16740"
     CDS             complement(<1..343)
                     /locus_tag="AC16CY_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3355499.1"
                     /translation="MSKRIFLFIVTNLAIVFTLSIVAALLGVGSGFGPEGLNLQGLAV
                     FCLFWGMGGAFVSLQMSRFIAKRATGVHLVDGRTGNSQADWLYETVGRLTRQANLPMP
                     EVGIYESPEVNA"
     gene            537..1895
                     /locus_tag="AC16CY_16745"
     CDS             537..1895
                     /locus_tag="AC16CY_16745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR3355500.1"
                     /translation="MTMWLPNLDGRKGPVYRAIADAIDEDVERGTLRAGARLPPHRDL
                     ADHLGLTVTTITRAYAEASRRGLTSGHVGRGTFIRGREIDDVDSDGLLDLGINILMPD
                     KEVAQLEVHLMRRRTLPWVQLLGYAPKRGHVRHRQAMAAWLAYLGLPVSPEQMVLTAG
                     AQHGLATTLAALVGPGETLLVEDLTYSGMRVLAQQLHVRVRGVAMDAEGLKPDALEAA
                     CRASRASVLYCMPRLQNPTSAVMSEKRRRQIAAVAEKCRLTVIEDDTYGFLSPERWPL
                     TRLIPHRCVFVTSLSKSLFPGMRLGCAVAPPAILDKIANAVWATMITTSSIGADLLSG
                     WIEDGTAARIADWKRHEGTARQGIAKRLLAGQRYQTHPSSHHIWLSLPPRWSSEGFVA
                     QARTRGVVLNASSEFAAGLEQPKAVRLCIGTPRTRATLEQALTRVSECLMDRAPAARA
                     VV"
     gene            complement(1899..3110)
                     /locus_tag="AC16CY_16750"
     CDS             complement(1899..3110)
                     /locus_tag="AC16CY_16750"
                     /EC_number="4.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008473 - ornithine cyclodeaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase, nickel-pincer
                     nucleotide-dependent"
                     /protein_id="HIR3355501.1"
                     /translation="MSVTDTIEAEGHLIDSGLLSAIFDKIIEVKGSYQIVTFDIGKTN
                     DDPSRIKMRVTVADEATLDDLLQQLTTFGAHPVRESNVLVKPAEKERCVPDDFYSTTN
                     HRTHVRLAGRWVEVERQRMDAVIVVEGKKAACRKLRDVKAGEKVVCGHDGIRVTPEFK
                     ERDRFGFAFMSNDVSSERRVEGSVARIAQMVRDVKAGGGRIAVVAGPVVVHTGGVEHF
                     SSIIRGGFVDVLLAGNALAVHDIEYALSGTSLGIDLVAGAPAEQGHRNHMAAINTINR
                     AGSIRAAVEQGVLTSGVMYECVKRGVDFVLAGSIRDDGPLPETVMDLVEAQERYASAL
                     ADNVQLVLMLSSMLHSIGVGNMLPSWVRVVCVDINPAVVTKLSDRGSLQTVGVVTDVG
                     LFLHRLAEALR"
     gene            complement(3295..>3696)
                     /locus_tag="AC16CY_16755"
     CDS             complement(3295..>3696)
                     /locus_tag="AC16CY_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3355502.1"
                     /translation="DGTAPPADILALLAERGVPGKQLHVFNDRTTVVISRENLHDEGQ
                     LRDALQARFGGRAQLADGLAAVSVIGAGINATYENVRTGAGTLGTLGIVPAGTATSSF
                     RTTWMVPDDRVKEAVRALHARFIEQPRPLLP"
BASE COUNT          635 a         1236 c         1208 g          617 t
ORIGIN      
        1 aggcgttcac ctcaggcgat tcgtagatgc cgacctcggg catcggcaga ttggcctggc
       61 gcgtcaggcg ccccacggtc tcgtagagcc agtcggcctg gctgttgccc gtacgtccat
      121 cgaccagatg gacgccggtg gcgcgcttgg cgatgaagcg cgacatctgc agcgacacga
      181 acgctccgcc catgccccag aagaggcaaa agactgcgag cccctgaagg ttcagcccct
      241 ccgggccgaa gccgcttccg acgccgagca gcgcggcgac gatcgacagc gtgaagacga
      301 tcgcgaggtt cgtgacgatg aacaggaata ttcgtttact catggcagtt tcaggatagc
      361 actggtatta agacgacggg aagcggtgcg agggtgcgaa ggtgcggaag gggggcgcgg
      421 aaacgcaccc tcgcactgtc gcaccctcgc accgtcgcac cgtcgcaccg caggcgtaag
      481 tacatttgcc agtaagacaa gtagattgtc agcgccgcaa tgtctatatt gcggtgatga
      541 caatgtggct gccaaacctg gacggccgaa aggggcccgt ctaccgggcc atcgccgacg
      601 ccatcgacga ggacgtcgaa cggggcacgc tgcgggccgg ggcccgcctg ccgccgcacc
      661 gcgatctcgc cgaccacctg gggctcacgg tcacgacgat cacccgggcc tacgccgagg
      721 cgtcccgccg ggggctcacc tccggccacg tcggccgcgg cacgttcatc cggggccgtg
      781 agatcgacga cgtcgacagc gacgggctgc tggacctcgg catcaacatc ctgatgccgg
      841 acaaggaggt cgcccagctc gaggtgcacc tcatgaggcg tcgcaccctg ccgtgggtgc
      901 agcttctcgg gtacgccccc aagcgcggcc acgtgcgtca ccggcaggca atggctgcct
      961 ggctcgccta ccttgggctg cctgtcagcc cggaacagat ggtgctcacc gccggggcgc
     1021 agcacggcct ggcaacgact ctcgccgccc tggtcgggcc gggagagacc ttgctcgtcg
     1081 aggacctcac ctattccggc atgcgggtgc tggcgcagca gctgcacgtg agagtgcgcg
     1141 gcgtcgcgat ggatgccgaa ggcttgaaac cggacgcgct cgaggccgcg tgccgtgcgt
     1201 cgcgggccag cgtcctctac tgcatgccgc ggctgcagaa ccccacgtcc gccgtcatgt
     1261 ccgaaaagcg gcggcgccag atcgcggcgg tggccgagaa gtgtcggctg accgtgatcg
     1321 aagacgacac ctacggcttc ctctcgccgg agcgatggcc gctgacgcgg ctgatcccgc
     1381 atcgatgcgt cttcgtgacg agcctctcca agagcctctt cccgggcatg cggctcgggt
     1441 gcgcggtcgc acctccggca atcctcgaca agatcgcaaa cgccgtgtgg gccacgatga
     1501 tcacgacctc gtcgatcgga gccgacctac tgagcgggtg gatcgaagac ggcacggcgg
     1561 cgcgcatcgc ggactggaag cggcacgagg gcacggctcg ccaggggatc gcaaagaggc
     1621 tgctcgccgg ccagcgctac cagacgcacc cgtcgagcca ccacatctgg ctctcgttgc
     1681 cgccccgctg gtcctccgaa gggttcgtcg cccaggcgcg cacgcgcggt gtcgtgttga
     1741 acgcgtcgtc cgagttcgcg gctggactcg aacagccgaa ggccgtgcga ctgtgcatcg
     1801 ggacgccccg tacgcgtgca accctcgagc aggcgctcac gcgcgtgtcc gaatgcctga
     1861 tggaccgcgc tcccgcggca agagctgttg tctgatcgct atcgcagcgc ttcagccagc
     1921 ctgtgcagga acagaccgac gtcggtgacg acgccgaccg tctggagcga tccccggtcg
     1981 ctgagcttgg tgacgaccgc ggggttgatg tccacgcaga ccacgcggac ccacgaggga
     2041 agcatgttgc ccacgccgat gctgtgcagc atggaggaca gcatcagcac gagctgaacg
     2101 ttgtcggcca gcgccgaggc gtagcgctcc tgcgcctcca ccaggtccat caccgtctcc
     2161 ggcagcgggc cgtcgtccct gatgctgccg gcgaggacga aatcgacccc gcgcttgacg
     2221 cattcgtaca tgacgcccga tgtcagcacg ccctgctcga ccgcagcccg aatgctgccg
     2281 gcgcgattga tcgtattgat ggccgccatg tgattgcgat gcccctgctc ggcgggcgca
     2341 ccggcgacga gatcgatccc gagcgaggtg cccgagagcg cgtattcgat gtcgtgcacg
     2401 gccagcgcgt ttccggccag caggacgtcc acgaagccgc cgcggatgat ggacgagaag
     2461 tgctccacgc cgcccgtgtg cacgacgacc ggcccggcca cgacggcgat gcgcccgccg
     2521 cccgccttca cgtcgcggac catctgggcg atgcgcgcga cgctcccctc gacgcggcgc
     2581 tccgacgaga cgtcgttgct catgaacgcg aaaccgaacc ggtcccgttc cttgaactcc
     2641 ggcgtgacgc ggatgccgtc gtgaccgcag acgaccttct ctccggcctt gacgtcgcgc
     2701 agtttgcggc aggccgcctt ctttccctcg acgacgatca cggcgtccat gcgctgccgt
     2761 tcgacctcca cccatcggcc cgccagccgc acgtgtgtcc ggtgattggt cgtggagtag
     2821 aaatcgtcgg gcacgcagcg ctctttttcg gccggcttga cgagcacgtt gctctcgcgg
     2881 accgggtggg cgccgaacgt agtgagctgc tgaagcaggt cgtcgagcgt cgcctcgtcg
     2941 gcgaccgtca cgcgcatctt gatccgtgag ggatcgtcgt tggtcttgcc gatgtcgaac
     3001 gtgacgatct ggtaggagcc cttgacctcg atgatcttgt cgaaaatcgc cgacagcagg
     3061 cccgagtcga tcaaatggcc ttcggcctcg atcgtgtccg tgactgacat caggccatca
     3121 ttctagacta cggcgtgaca ttggtgattt ctgattggtg attgctgaat cgttgttgat
     3181 tgaagattgt tgattggcga ttgggctctc aatcaccaat ctcaaatcac aaatcatcaa
     3241 cgaaccaaca atcaccaaaa tcaccaatcg caaatcaact ggcgtctcgc tcagtcaggg
     3301 cagcagtggc cgcggctgtt cgatgaaacg cgcgtggagg gcgcgtaccg cctctttcac
     3361 gcgatcgtca ggcaccatcc aggtggtgcg gaacgaggat gtcgccgtgc ctgcgggaac
     3421 gatgccgagc gtgcccagcg tcccggcccc ggtgcggacg ttctcgtagg tcgcgttgat
     3481 cccggcgccg atcacgctga cggcggcgag cccgtcggcg agctgcgccc gccccccgaa
     3541 ccgcgcctgc aaagcgtccc tgagctgtcc ctcgtcatgc aggttctcgc gcgagatcac
     3601 gacggtcgtc cggtcgttga agacgtgcag ctgtttgcct ggaaccccgc gctcggcgag
     3661 cagcgcgagg atgtcggcgg gcggggcggt tccgtc
//
LOCUS       DBNGSH010000732         2523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2456917, whole genome shotgun sequence.
ACCESSION   DBNGSH010000732 DBNGSH010000000
VERSION     DBNGSH010000732.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2456917"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1238
                     /locus_tag="AC16CY_16760"
     CDS             60..1238
                     /locus_tag="AC16CY_16760"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3355503.1"
                     /translation="MFRALIGVMAAAALALSSAAVVTGDTIFRSALHDYRVVTLVDAL
                     VQPWSIAFIPGGDILITERPGRLRIVRQGKLLPQPVEGVPKVFFSSQGGLLEVMPHPN
                     FASNRLIYLTYSKPGETDNQARTALVRGRLENDRLTGVEQLFEAVAQGRNHFSGKIAF
                     DRNGYLFLSLGDRQVPPEGNLEAHPAQDLSNHHGKIVRLHDDGRVPSDNPFVSRAGAR
                     SEIWSYGHRNVQGLAIHPETGDVWADEHGPQGGDELNRIQPGRNYGWPVIGYGVNYTT
                     GLAIHAGTHRQGMEQPVHVWVPSIGVSGMMIYTGDRFPEWRGNLFVGGMAGQQLVRLT
                     LNGQRVANAETLVQQMGRIRDIRQGPDGFIYLVTDDRDGKPTPVFRLEPVARAPVAEK
                     "
     gene            1274..1885
                     /locus_tag="AC16CY_16765"
     CDS             1274..1885
                     /locus_tag="AC16CY_16765"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIR3355504.1"
                     /translation="MATVIFVAEHAMDEAACRRVREAMNEGASEPAEILGGEIEVAEH
                     ERRASHIEVSEQVLGLVEACLDSQREAIGAFFGRTLRSREGPGLLRYATGGFYGPHVD
                     RAEVASWPQAAERAVAVVLFLESSRDVDAAGGFSGGLLRVYGDGDGAAPVDIVPTRGT
                     LVAFPADTLHEVTTVLDGTRDIVVDWFRESRVSERQTVPIANR"
     gene            complement(1848..>2523)
                     /locus_tag="AC16CY_16770"
     CDS             complement(1848..>2523)
                     /locus_tag="AC16CY_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3355505.1"
                     /translation="VEPLTLAVASDSDPVWSPDGRRVAFRSLQKGRPALFTKRVRAAD
                     AEDELLLDADATPSDWRGSGVLLHEADPASGQDIVTIDAVRRSRAHVVKSGFNDTDGR
                     QSPDGRWLAYVSDESGRPDIYLRPWPDGDRIRVSMAGGTRPRWSRDGRSLFFLRGSTI
                     MRATLRDGSPTRIDPVAVTDVPGLRDFDVAHRRDALLALVPVAGASSAPVSAIIDWQS
                     VLSDAR"
BASE COUNT          428 a          836 c          861 g          398 t
ORIGIN      
        1 ggcccccaat aatcatccga ctgtctttcg cggggcagat ctcaaccgag gagacgacga
       61 tgttccgagc gctaatcggc gtgatggcgg cagccgccct ggccctgtcg tcggcggcag
      121 tggtgactgg cgacacgatc ttcaggtccg ctcttcacga ctacagagtc gtgaccctgg
      181 tagacgcact cgtgcagccg tggtccattg cattcattcc cggcggagac atcctgatca
      241 cggagcgacc cggccggctc aggattgttc ggcaggggaa actccttccg cagcccgtcg
      301 agggcgtgcc gaaggtgttc ttctcgtccc agggcggact gctcgaggtc atgcctcacc
      361 cgaacttcgc ctcgaacagg ctgatctatc tgacctactc gaagccgggt gagaccgaca
      421 accaggcaag gaccgcgctc gtgcgcggcc ggctcgaaaa cgatcggttg accggcgtcg
      481 agcagctctt cgaggccgtc gcgcagggac gcaaccactt cagcggcaag attgccttcg
      541 acaggaacgg ctacctcttc ctgtcgctcg gcgaccggca ggtgccgccc gaaggcaacc
      601 tcgaagcgca cccggcgcag gatctgtcga accatcacgg caagatcgtc cggctgcacg
      661 acgacgggcg cgtgccgtcg gacaacccgt tcgtaagccg ggccggcgcg cggtccgaga
      721 tctggagcta cggccaccgc aacgttcagg ggctcgccat tcatcccgag accggggacg
      781 tctgggcgga cgagcacggc ccgcagggcg gggacgagct caatcgcatt cagccgggcc
      841 ggaactacgg gtggcccgtc atcggctacg gcgtcaacta cacgaccggg ctcgcgattc
      901 atgccggcac gcaccgccag gggatggagc agccggtcca cgtctgggtc ccgtcgattg
      961 gtgtgtcggg gatgatgatc tacaccggcg accgcttccc cgagtggcgc ggaaacctct
     1021 tcgtgggcgg gatggcgggc cagcagctcg tgcggctgac gctcaacggt cagcgggtcg
     1081 ccaacgcgga aacgctggtg cagcagatgg gacgcatccg ggatatccgg cagggaccgg
     1141 atgggttcat ctacctcgtc accgacgatc gagacggcaa gcccacgccg gtctttcgac
     1201 tggagcccgt cgcgcgggca ccagtcgccg aaaagtagag cgagtacttc acccgacccg
     1261 ctgcgcggag gccgtggcca ccgtcatctt cgtcgcagaa cacgccatgg acgaggccgc
     1321 ctgccgccgc gtacgggagg cgatgaacga gggcgcgtcc gagccggcag aaattctcgg
     1381 cggcgagatc gaagtcgcgg agcacgagcg ccgggcgtcc cacatcgagg tgagcgagca
     1441 ggtgctcggt ctggtcgagg cgtgtctcga ctcccaacgt gaggcgatcg gcgccttctt
     1501 tggccgaacg ctgcgatcgc gcgagggtcc tggcctgctc cgttatgcca cgggaggctt
     1561 ctacggtccg cacgtggacc gcgcggaggt tgcgtcgtgg ccacaggcgg ccgagcgcgc
     1621 ggtcgcagtc gttctgttcc tcgagagctc gcgtgacgtg gacgcggctg gtggattcag
     1681 cggcgggctt ctccgtgtgt acggcgacgg cgatggtgcc gcgcccgtgg acatcgtgcc
     1741 gacccgcggg acgctggtgg cattcccggc cgacacgctg catgaggtga cgacggtgct
     1801 ggacggcacg cgtgacatag tcgttgattg gttccgtgaa agcagggtca gcgagcgtca
     1861 gacagtaccg attgccaatc gatgatcgcc gacaccggcg cagacgaagc gccggccacc
     1921 ggcaccaggg cgagcagcgc atcccttcgg tgagccacgt cgaaatcccg aaggcccggc
     1981 acgtcggtca cggccactgg gtcgatgcgc gtgggagacc cgtcccgcaa tgtcgcgcgc
     2041 atgatcgtcg agcccctgag gaagaagagc gagcggccgt cgcggctcca gcgcgggcgc
     2101 gtgccccctg ccatcgacac ccggatgcga tcgccgtcgg gccacggccg taggtagatg
     2161 tcggggcggc cggattcatc cgacacgtat gccagccacc ggccgtcggg cgactgacgc
     2221 ccgtccgtgt cgttgaagcc gctcttcacg acgtgcgccc gcgaccgtct gacggcatcg
     2281 atggtgacga tgtcctggcc cgatgccgga tccgcttcgt gcagcagcac gcccgaaccc
     2341 cgccagtcgc ttggtgtggc gtcggcgtcg agcagcagct cgtcttccgc atccgccgcc
     2401 cgaacgcgct tggtaaaaag cgccggccgg cctttctgca gcgatctgaa cgccacgcgg
     2461 cgtccgtcgg gcgaccacac cggatcgctg tctgaggcga cagccaacgt caacggttcg
     2521 aca
//
LOCUS       DBNGSH010000733         1781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2456962, whole genome shotgun sequence.
ACCESSION   DBNGSH010000733 DBNGSH010000000
VERSION     DBNGSH010000733.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2456962"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC16CY_16775"
     CDS             complement(<1..386)
                     /locus_tag="AC16CY_16775"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3355506.1"
                     /translation="MANLGFIGLGAMGSRITKRLLAKGHAVTGYDLFPAKQPELVAMG
                     GKWASSPRAVCEAADTTFVMVTNTAALEGASQGPDGLLAGLGPGKLLIDMSTVGPAAS
                     RALAAKVREKGADMLDAPVSGSTSMV"
     gene            502..>1781
                     /locus_tag="AC16CY_16780"
     CDS             502..>1781
                     /locus_tag="AC16CY_16780"
                     /inference="COORDINATES: protein motif:HMM:NF014697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HIR3355507.1"
                     /translation="MHDTPVRSTLASLGLRLSDWFERWFPDAFALALAAVVIVFLASL
                     ATGSGIVQTAQWFGAGFWDLVAFTMQMSMIIVTGYAVASSPPVYAVIRRLAAIPRDGR
                     TAAAYVGLFSMLASLVSWSFSLIFSGLLAKEVAHRVQGADYRAIGAAAYLGVGSVWAL
                     GLSSSAALIMAAPGSLPDSIERISGVIPLGQTLGLWQSLLIAALLIIVSMAVSYYSAP
                     GDADARGMKDMGVEYTAATQERSARETPGEWLEYSPVLTIAICTFGFLYMAREVAGNG
                     PAIILDLNHYVFLFLIVGLLLHWRPRSFVQAITAAVVPVGGVLIQYPLYAGIVRMMTE
                     SGIATQLAHFFVAISTPHTYPVMVGIYSAFLGLFIPSAGGKWLIEAPYMLEAAKTLQV
                     HLGWVVQTYNATEALANLIHPFWMLPLVGILGLKA"
BASE COUNT          255 a          596 c          594 g          336 t
ORIGIN      
        1 tcgaccatcg aggtgctgcc cgagaccggc gcgtcgagca tgtcggcgcc cttttcccgc
       61 accttcgctg ccagcgcgcg gctggccgct gggccgaccg tgctcatgtc gatgagcagc
      121 ttgccggggc cgaggccggc cagcagccca tccggtccct gcgacgcgcc ttcgagcgct
      181 gcggtgttgg tcaccatgac gaacgtcgtg tcggcggctt cacaaacggc gcgaggcgag
      241 gacgcccact tgccccccat tgcgacaagc tccggctgct tcgcaggaaa gagatcgtag
      301 ccggtcaccg cgtggccctt ggccagcaat cgtttggtga tccggctgcc catggccccg
      361 aggccgatga atccgaggtt tgccataatg cagtcgcacg ggcccgcggt cagccgatcc
      421 gaccagtaaa tacggccctt ccgtagagac gacagcttgt aagtatcccg tagaagcaaa
      481 ggctgaggca aggtgccgca gatgcacgac acgcccgtcc gctcgacact cgcctcgctc
      541 ggccttcgtt tgtcggactg gttcgagcgc tggtttcccg acgcgtttgc gctggcgctc
      601 gcggcggtgg tcatcgtctt tctcgcatcg ctcgccacgg gcagcggcat cgtccagacc
      661 gctcaatggt tcggcgcggg cttctgggat ctggtcgcct tcacgatgca gatgtcgatg
      721 atcatcgtga ccggctacgc cgtggcgagc tctccgccgg tgtacgccgt catccgaagg
      781 ctcgccgcga ttccgcgcga cgggcgcacc gcggcggcct acgtcggtct cttctcgatg
      841 ctcgcctcgc tcgtgtcgtg gagcttcagc ctgatcttca gcggcctgct ggcgaaggag
      901 gtcgcgcatc gcgtgcaggg ggcggactat cgcgcgatcg gcgccgcggc gtatctgggc
      961 gtcggcagcg tctgggcgct cgggctctcc tcgtcggcgg cgctcatcat ggcggcgccg
     1021 ggatcgcttc ccgactcgat cgagcgcatc agcggcgtca tcccgctcgg ccagacgctc
     1081 gggctctggc agagcctcct catcgcggcg ctgctcatca tcgtctcgat ggcggtgagc
     1141 tactactcgg cgccgggcga cgccgacgcg cgagggatga aggacatggg cgtcgagtac
     1201 acggcggcga cgcaggagag gagcgcgcgc gaaacgccgg gcgagtggct cgaatacagc
     1261 ccggtgctca cgatcgccat ctgcacgttc gggtttctct acatggctcg cgaagtcgcg
     1321 ggcaacggcc ccgcgatcat cctcgatctg aaccactacg tcttcttgtt cctcatcgtc
     1381 ggcctgctcc tgcactggcg gccccgttcg ttcgtgcagg cgatcacggc ggcggtggtg
     1441 ccggtgggtg gagtgctgat ccagtacccg ttgtacgcgg ggatcgtccg gatgatgacc
     1501 gagtcgggta tcgccacgca gctggcgcac ttcttcgtcg ccatctcgac gccgcacacc
     1561 tacccggtga tggtcggcat ctactcggcg tttctcgggt tgttcattcc gtcagcgggg
     1621 ggcaagtggc tgatcgaggc gccctacatg ctggaggcgg ccaagacgct gcaggtccac
     1681 ctcgggtggg tggtccagac ctacaacgcc acggaggcgc tggcgaacct gatccacccg
     1741 ttctggatgc tgccgctcgt cggcatcctg ggcctgaagg c
//
LOCUS       DBNGSH010000734         8647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4324720, whole genome shotgun sequence.
ACCESSION   DBNGSH010000734 DBNGSH010000000
VERSION     DBNGSH010000734.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4324720"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1242)
                     /locus_tag="AC16CY_16785"
     CDS             complement(151..1242)
                     /locus_tag="AC16CY_16785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3355508.1"
                     /translation="MLRNHEWPRWSMKVALASTIAGACRLPVLDGFVRARRPLILGYH
                     RVVENFAVAALTEMPNMLISVSMFERHLEWLGRQFRFVSLDDIGEHAVSGVPFDGPVA
                     AITFDDGYRDVYEQAYPTLKRMGIPAAVFVVTDLVGGDGCQIHDKLYHLLARAFARWD
                     DPRRELLGVLEELEIPSGHLFPTRATTRSPLLTVTALLPSLTQRDVRRVLAYLESVVG
                     PAASDMPRSLTWQMIAEMGRAGFTIGSHTKTHASLPLETAEAAVEEIAGSKRALEAHL
                     GKPVVHFAYPGGQFTPGVVDAVAAAGYQCGYTACPHHDPRHPTVTMERLLLSEGSSID
                     ATGRFSPPILSCQVHDLWPPARRCEWNHA"
     gene            complement(1232..2389)
                     /locus_tag="AC16CY_16790"
     CDS             complement(1232..2389)
                     /locus_tag="AC16CY_16790"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3355509.1"
                     /translation="MVHLMNAVSPDELRSPTVVEIIDRPSDFEALRPQWNELLSASAT
                     ASPFLTWEWVYAWWTHLGERSRLNLFVVRTGGQIVAIAPLRIARRPLTWLSSLEFLGT
                     GAAGSDYLDLIVRRGCEAVAVEALAGAFQSQGLTLRLSHLPPGSLALRLAGQMEHGGW
                     TSREAEAGVCPFIRLAGHTWESYLASLGSAHRANVRRRIREVARTFDVRFELVAGSAD
                     RLDVLSALAAFHDRRWSGDRGSTTFATPELHAFHQDATRRALDAGYLRLYGLRLDGQL
                     AGAMYAFSHAGRFYFYQHGFDQRYRQYSLGLFLMALTIRAAIDEGAVEFDLLYGIESY
                     KRLWTAETRPLGRLDLFPARVGGRLHRRRVDAESALRALAQRMRSRDGHAA"
     gene            complement(2377..3561)
                     /locus_tag="AC16CY_16795"
     CDS             complement(2377..3561)
                     /locus_tag="AC16CY_16795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3355510.1"
                     /translation="MSTAASTEPIRLLKLLPTFFCGGTENQFMTLSRSLDRHRFDVEL
                     ACLRRVGPFLAEAEGRSRPLREYPIDTFRSVRAVAQQARLARHIVQRRIEIVHAYSFY
                     GNVFAIPPSRLAGVPVVIASIRDRGPYLTPMQMRVQRYVCRLATRVLVNADAVKDWLL
                     DQGYDPARIVVIRNGVDLCRFNAPGDPDAIRRELGLAPGVPLVTVVSRLNHLKGVEDF
                     LRAAAMLADRYPTVHFLVVGEAGEPDVHYLTVLTRLADSLGIGGRVRFTGLRSDVPAV
                     LASSTVSVMPSLDEALSNVVLESMAAGVPTVATRVGGTPEAIADGVTGLLVPPGDATA
                     MAEAIARVLKDPGLAAALGCAGRRRIEERFSVDRMVEATEQLYVNLLAEKRPGRVVAW
                     ST"
     gene            complement(3562..4887)
                     /locus_tag="AC16CY_16800"
     CDS             complement(3562..4887)
                     /locus_tag="AC16CY_16800"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3355511.1"
                     /translation="MIASEATTAWWRPERAAPSGLTAQPGGRLAFGALFAFTGILILS
                     PQAWFPAFRSLRIALLAAGVAIIAHLLGGVIRRRPGQRHPEVVIAFCLAAWAVLTVPI
                     SLWPGGSVSVLTDLFLKAIVFFWLIGVLVTTGPRLRMFLRLLVVCSIPLAVTAVRNYR
                     SGVFLEASGAVMRIAGYNVGGSGLTENPNDMALMLNLLIPFAAALLAIERRLAWRVVA
                     ASALLLSIAAVVATFSRAGFLTLVAIGIVGLVTMARRRPVAALGIVLVLAASSPLIPR
                     GYFERLSTITNISSDPTGSSQGRWTDLTVAAEFVARHPLTGVGLGQNVLALNRERGPT
                     WREVHNVYLQYAVDLGLPGLALFLWLFVALFRAAGRVRRLAVSNTACRDVGIVAGGVQ
                     VALVGFAVAAMFHPVAYQFYFFCIAGLALAVKNVFSAAVASSRLAQQPV"
     gene            complement(4847..5839)
                     /locus_tag="AC16CY_16805"
     CDS             complement(4847..5839)
                     /locus_tag="AC16CY_16805"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3355512.1"
                     /translation="MKLAALDVFVCPACRSDLRLHNASQDGLEILEGRLTCSGCGVVY
                     PITRGVPRFVPTASYAQSFGRQWHWFRGVQMDSVNRTSESEAALCATTGWRDDEFPGR
                     RLLDAGVGAGRFAERAAAKGAEVFGVDLTLAVDAAYRNIGHLSNVHLAQADIFALPMR
                     ASTFDLAYSIGVLHHTPDPPTAFARVADTLAPGGKLAVYVYARYGPGHHMSDAIRVVS
                     TRLPLRVSWALAAGAVPLYYLYRIPVVGKACRLALPISMDPRWRWRWLDTFDWYTPTY
                     QTKYLYPEVFRWFRQQGFEVVDLLDGPIRMSGVKSHSQEGTRDRERSDHRLVAS"
     gene            complement(5836..7113)
                     /locus_tag="AC16CY_16810"
     CDS             complement(5836..7113)
                     /locus_tag="AC16CY_16810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HIR3355513.1"
                     /translation="MNLVTTLRRHPVLMCVSATALAPVVVVLSTYMLPTVAWQMPTSI
                     ATVVVLVGLAVAVETPVRNLFVKRVLILGANSTASKLIEEIESPRERRFVALGVVDRI
                     IPSGPIASRWLGCFDELASIVGRLRPTHIVLARNDRRANLPLDTLLQSRVRGILVEDT
                     IDFDERLTGAMAIEALTPDRLVLSKGFRNSGTMQGTARALSVITATVGLVVVAPLLAI
                     IAVAVKLDSRGPVFFLQSRAGRDGRPFQLIKIRTMRSSSGLRSEWIKDNEDRVTRLGK
                     WLRRFRLDELPQFLNVLRGEMNLIGPRPHPSSNTRLFEERIPFYGLRSAVLPGITGWA
                     QVRYGYANTLEEETEKMRYDLYYIKHRSLWLDVRIVIETIGVMLRGGGATRVRRPTGV
                     RRPVSRLTERRSIADAMPAEAWIAKGAPSAGRP"
     gene            complement(7194..7877)
                     /locus_tag="AC16CY_16815"
     CDS             complement(7194..7877)
                     /locus_tag="AC16CY_16815"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR3355514.1"
                     /translation="MPRQIDAALVSLTAPNTFAAEKYQGLRLRIEQLKHERGVTVLAV
                     TSPDISAGKTVTSINLAVALARGAGARVLLIDSDLRRPAVGAHLGLHGVKGAGLAEGL
                     GDEHIELRQIVRPLSPGSTVAVILAGAVPTHVSELIRSRRFEILLQEARALYDFVIID
                     TPPLAPVYDAAVLSRLVDGILIVVAAHETPRKLLEETLNLVDGSKVLGMVYNGDDSSQ
                     SQYDAYYTR"
     gene            complement(7905..>8647)
                     /locus_tag="AC16CY_16820"
     CDS             complement(7905..>8647)
                     /locus_tag="AC16CY_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355515.1"
                     /translation="QLDGFPDRNPDVRRIKDEIAALEKAGGTGPAGSSVAGPARTAVT
                     AGRGSGGARTTESLDAEIASLKAAEASLRQTIEMVERRLDRAPHRQNEFATMSRDHQA
                     AREHYASLQKEYEAAQLAENMETSSQGDRFRIMEAAVPPTGPSGPNRVRLLIMALFAA
                     ALAAGLVVLLLDQFDTSFHSVDDLRRFTKIPVLVAVPRIGPSGAARWRRGALLTASVL
                     VCFVLLGMLSAYVAHDNASLVRLLVRGG"
BASE COUNT         1554 a         2871 c         2939 g         1283 t
ORIGIN      
        1 ggaacagctg tttgtacgtc ccaaacgcgg cctggtcgag gagccggacg atcacgaggg
       61 ggacgacgac ggccgccagc agccccaccc cgcggccgac gatgagccac agcgcgggcc
      121 gcgacacgcc gtccagccgg agcaggtctc tcatgcgtga ttccattcgc accggcgggc
      181 gggcggccag agatcgtgaa cctggcagct cagaatgggc ggagagaacc tgccggtcgc
      241 atcgatcgag gacccctccg agagcagcag gcgctccatc gtgaccgtgg gatgccgtgg
      301 gtcgtgatgc ggacatgcgg tgtagccaca ctggtacccg gcggccgcca cggcgtccac
      361 gacccccggg gtgaactgcc cgccgggata ggcgaaatga acgacgggtt ttccaaggtg
      421 cgcctccagc gcgcgcttcg atccggcgat ctcttcgacg gcggcctccg ccgtttccag
      481 gggcaacgac gcatgcgtct tcgtgtgcga gccaatggtg aagccggccc gacccatttc
      541 ggcaatcatc tgccatgtga gcgagcgcgg catgtcgctc gcggccggcc cgacaaccga
      601 ctcgaggtac gccagcacgc gcctgacatc ccgctgtgtg agcgacggaa gcagtgccgt
      661 caccgtcaac agggggctcc tggtcgttgc gcgcgtcggg aacagatgcc ctgacgggat
      721 ctcgagctcc tccagcacgc cgaggagctc gcgacggggg tcgtcccacc gcgcgaacgc
      781 cctggccagg aggtgataca gcttgtcgtg gatctggcag ccatcgcccc cgacaaggtc
      841 ggtcacgacg aagacagcgg cggggatgcc catccgcttg agcgtcgggt aggcctgctc
      901 gtacacgtcg cggtacccat cgtcgaaggt gatggccgcg accggaccgt cgaacgggac
      961 gccgctgacg gcgtgctcac cgatgtcgtc gaggctgacg aagcggaact gacgtccgag
     1021 ccactcgagg tggcgctcga acatcgacac gctgatgagc atgttcggca tctcggtgag
     1081 ggcggcgacg gcgaagttct cgacgacccg gtgatagccg agaatcaacg gcctgcgcgc
     1141 gcgcacgaac ccgtccagca ccggcagacg acatgcgccg gcgatggtcg atgccagcgc
     1201 caccttcatc gaccaccgcg gccactcgtg gttacgcagc atggccgtcc ctcgaccgca
     1261 tccgttgcgc aagggcacgc aaggccgact ccgcgtcgac cctccgtcga tgcagtctgc
     1321 cgccgacgcg ggcaggaaac agatcgagac gaccgagtgg gcgtgtctcg gccgtccaca
     1381 gccgcttgta ggactcgatc ccatacagca ggtcgaactc gaccgcgccc tcgtcgatgg
     1441 cggcgcggat cgtgagcgcc atcaggaaca ggcccagact gtactggcgg taccgctggt
     1501 cgaagccgtg ttgatagaag tagaaccggc cggcgtgcga gaaggcgtac atcgcgccgg
     1561 ccagctggcc gtcgaggcgg agcccgtaga gccgcaggta gccggcatcg agagcgcgtc
     1621 gggttgcgtc ctgatgaaag gcgtgaagct ccggtgtcgc gaacgtcgtg gagccgcgat
     1681 cgccggacca gcgccgatca tgaaacgcgg caagcgccga cagcacgtcg agtcgatcag
     1741 cggaaccggc gacgagttcg aaccggacgt caaaggtccg cgccacttcc ctgatccgac
     1801 ggcgcacgtt tgcgcgatgc gcggatccca gactcgccag gtacgactcc caggtgtgcc
     1861 cggccaggcg aatgaagggg cacactcccg cctcggcttc ccgagaggtc cacccaccgt
     1921 gctccatctg tccagcaagt cgcaaagcca gtgaacccgg aggcagatga ctgagtcgaa
     1981 gcgtgagccc ctgtgattgg aaggctccgg ccaaggcctc gaccgccaca gcttcgcacc
     2041 cgcgccggac gatcaggtcc agatagtcgg agccggcggc gccggtgccc aggaactcca
     2101 ggctcgacag ccacgtcaga gggcgacggg cgatccggag cggcgcaatg gcgacgatct
     2161 ggccgccggt tcggacgacg aacaggttga gacgcgaccg ttcaccaaga tgggtccacc
     2221 acgcgtacac ccattcccat gtgaggaacg ggctcgcggt cgcgctggcg ctgagcagct
     2281 cgttccactg tggccgcagt gcctcgaagt cgctcggccg gtcgatgatc tcgaccaccg
     2341 tcggcgaccg cagttcgtca ggcgagacgg cgttcatcag gtggaccatg ccaccacccg
     2401 tccagggcgt ttctcggcga ggaggttcac gtacaactgc tccgtcgcct cgaccatccg
     2461 gtccaccgag aaccgctctt cgatccgtcg ccgtcccgcg cagccgagcg cggcagccaa
     2521 cccgggatcc ttgagcactc gcgcgatcgc ctcggccatc gcggtggcat cgcccggggg
     2581 cacgaggagg cccgtcaccc cgtccgcgat ggcctccggt gtcccgccta cccgcgtcgc
     2641 gacagtcgga acgccggccg ccatcgattc cagcacgacg ttggacaagg cctcatcgag
     2701 cgacggcatg acggagaccg tggagctcgc cagcacggcg ggcacgtcgg atcggagtcc
     2761 ggtgaagcgc acacggccgc caatcccaag gctgtcggcc aggcgggtga gcaccgtcag
     2821 atagtgaacg tccggttcgc cggcctcacc gacgaccagg aagtggactg tcgggtagcg
     2881 atctgccagc atcgccgccg cgcgcaggaa gtcctcgacg cccttcagat ggttcagccg
     2941 cgagacgacg gtcacgagcg gcacaccagg cgcgaggccg agctctcgcc ggatggcatc
     3001 gggatcgccc ggcgcgttga agcgacagag gtcgacgccg tttcgaatca ccacaatcct
     3061 cgccgggtcg tacccctgat cgagcagcca gtccttgacg gcgtccgcat tcaccaggac
     3121 gcgcgtggcc aggcggcaga cgtaccgctg cacccgcatc tgcatgggcg tcaggtacgg
     3181 cccgcggtcg cgaatcgacg cgatcacgac cggcacgcct gcgagcctgg aaggcggaat
     3241 ggcgaagacg ttcccgtaga agctgtacgc gtggacgatc tcgatgcgac gctgcacgat
     3301 gtgcctcgcc aggcgtgcct gctgcgcgac cgcccgcaca ctgcgaaacg tatcaatcgg
     3361 gtactcgcgc agcggccggc tgcggccctc ggcctccgcg aggaatggtc ccacgcgcct
     3421 gaggcacgca agctccacgt cgaatcggtg gcggtcgagt gaccggctca gcgtcatgaa
     3481 ctggttctca gtaccgccgc agaagaacgt cggcaggagt ttcaggagac ggatcggctc
     3541 ggtcgatgcg gctgtgctca tttacaccgg ctgttgcgcc aggcgagacg acgcgactgc
     3601 tgcgctgaag acgttcttga ccgccagtgc cagaccggcg atgcagaaga agtagaactg
     3661 gtaggcgaca gggtggaaca tggctgcgac ggcaaacccg acgagggcga cctgaacgcc
     3721 gccggccacg atgcccacgt cgcggcatgc cgtgttggag acggcgagtc gcctgacccg
     3781 cccggcggcc cggaacagcg cgacaaagag ccagaggaag agcgccagcc cgggcaggcc
     3841 cagatccacg gcgtactgga ggtacacatt gtggacttcc cgccacgtgg ggccccgctc
     3901 ccggttgagc gcgaggacgt tctgaccaag tccgacgccg gtgagcgggt ggcgcgcgac
     3961 gaactcagcg gccacggtca ggtcggtcca ccgtccctgg gaggacccgg tgggatccga
     4021 cgagatgttc gtaatcgtgc tgagtcgctc gaagtatccc cggggtatga gaggcgagct
     4081 tgccgcgagg accaacacga tgcccagtgc cgccacgggg cgccgcctgg ccatcgtgac
     4141 caggcctacg atcccgattg cgacgagcgt gaggaagccg gcgcgcgaga acgtggcaac
     4201 gacggcggcg atgctcagca gcagggcact tgcggccaca acgcgccacg ccagtcgccg
     4261 ttcgatcgcg agcagggccg ccgcaaacgg aatcaggagg ttgagcatca acgccatgtc
     4321 gttcgggttc tctgtcagcc ccgagcctcc gacgttgtag ccggcgatcc gcatgacggc
     4381 tccgctcgcc tccaggaaca cgccggaccg gtagttccgg actgctgtca ccgcgagggg
     4441 tatcgagcag acgacgagca gccggaggaa catccgcagc cggggaccgg tggtgaccaa
     4501 cacgccgatc agccagaaga acacgattgc cttgagaaag agatccgtga gtaccgagac
     4561 gctgccccct ggccacagcg agatgggcac cgtcaacacc gcccacgcgg cgaggcagaa
     4621 cgcgatcacg acttcggggt gcctctggcc cggccgccgg cggatgacgc cgcccaacag
     4681 gtgcgcgatg atggccaccc ccgccgcgag cagcgcgatg cgcaggctcc tgaacgccgg
     4741 gaaccatgcc tgtggcgaca gaatcagaat gccggtgaac gcgaacaacg cgccgaaggc
     4801 gagacggccg cccggctgtg cggtcagacc ggaaggcgcg gcgcgttcag gacgccacca
     4861 ggcggtggtc gcttcgctcg cgatcacggg ttccctcctg tgagtggctc ttgactccgg
     4921 acattcggat cggaccgtcc aggagatcga cgacctcgaa cccctgctga cgaaaccagc
     4981 gaaacacctc agggtagagg tacttcgtct ggtacgtcgg cgtgtaccaa tcgaaggtat
     5041 ccagccaccg ccagcgccat cggggatcca tggagatcgg cagcgcgaga cggcacgcct
     5101 ttccgacaac cggaattcga tagaggtagt acagcgggac cgccccggcc gccagcgccc
     5161 agctcactct tagcggcagg cgggtgctga cgacgcgaat cgcgtcggac atgtggtgac
     5221 ccggtccgta gcgcgcgtag acgtagaccg ccagcttgcc gccgggcgcg agcgtgtccg
     5281 ctaccctggc aaatgcggtt ggcggatcgg gcgtgtgatg cagcacgccg atggagtagg
     5341 cgaggtcgaa cgtgctcgcc cgcatcggga gtgcgaagat gtcggcctgc gcgaggtgga
     5401 cgttgctgag gtgtccgata ttgcggtacg cggcgtcgac agccagcgtg aggtcgacgc
     5461 caaagacctc ggcccccttc gcggcggcgc gctccgcaaa gcggccggcg ccgacaccgg
     5521 catcgagcaa ccgccgcccg ggaaactcgt cgtcgcgcca accggttgtc gcacagaggg
     5581 cggcctcgga ctcggacgtg cggttgacgg agtccatctg tacgccgcga aaccagtgcc
     5641 actggcgacc gaagctctgc gcatacgacg cagtgggcac gaatcgcggc actcctcgcg
     5701 tgattggata caccacgccg catccactgc aggtcaggcg cccctcgaga atctcgaggc
     5761 cgtcctggga tgcgttgtgg agacgcagat cgctgcggca ggcaggacag acgaacacat
     5821 ccagggcggc gagcttcatg gccggcctgc cgagggtgcg cccttggcga tccaggcctc
     5881 ggccggcatc gcatccgcga tggagcgccg ctcggtcagc cgtgacacgg gacgccgaac
     5941 tcctgtcggg cggcggaccc tcgtcgcacc accgccccgc agcatgacgc caatcgtctc
     6001 gatcacgatg cggacgtcga gccacagcga gcgatgcttg atgtagtaca agtcgtagcg
     6061 catcttctcg gtctcctcct cgagggtgtt cgcgtagccg tagcgcacct gcgcccatcc
     6121 ggtgatcccg ggcaacaccg ccgaccgaag cccgtagaac ggaatccgct cctcgaacaa
     6181 tcgggtattg ctgctcgggt gcgggcgggg accgatgaga ttcatctcac ctcgcaagac
     6241 gttcaggaac tgtgggagct catcgaggcg aaaacgtcgc agccacttgc cgaggcgtgt
     6301 cacgcgatcc tcgttgtctt tgatccattc ggatcggagc ccgctgctcg accgcatcgt
     6361 cctgatcttg atcagctgga acggacggcc atcccgtccg gcgcggctct gcaggaagaa
     6421 cacgggaccg cgcgaatcca gcttgacggc gaccgcgatg attgccagca ggggcgccac
     6481 cacgaccaga ccaacagtcg ccgtgatgac gctcagcgca cgggcagtgc cctgcatggt
     6541 cccggaattc ctgaatccct tcgacagcac gagccggtcc ggcgtgaggg cctcgatcgc
     6601 catcgccccc gtcaagcgct cgtcgaagtc gatcgtgtcc tcgacgagga tgccccgcac
     6661 tcgggactgc agcagcgtgt cgagcgggag gttcgcgcgt cgatcgttac gggccaggac
     6721 gatgtgcgtc ggtcgcaggc gcccgacgat ggaggcgagc tcgtcgaagc agccgagcca
     6781 ccggctggcg atcgggccgc tgggaatgat ccgatccacc acacccaggg ccacgaaacg
     6841 ccgctcgcgt ggtgactcga tctcttcgat cagcttggat gcagtcgagt tggctcccag
     6901 gatgagcacg cgcttcacga agaggttccg caccggggtc tcgaccgcca cggcgagtcc
     6961 gacgaggacg accacggtcg cgatgctggt cggcatctgc cacgcgaccg tcggcagcat
     7021 gtaggtcgac agcactacta ccaccggtgc gagggcggtg gcggagacgc acatgaggac
     7081 ggggtgtcgg cggagcgtag tcacaaggtt caccggggcg tgatggatcg gagtcggagc
     7141 gtcggcgacg gggagaggcg gcctctcgcg tggcgtcgat ctgcgcgggg gcgtcagcgt
     7201 gtgtagtacg cgtcgtactg tgactgcgag gagtcgtcgc cgttgtacac catgccgagc
     7261 actttggacc cgtcaaccag gttcagcgtc tcttcgagca gcttccgcgg cgtttcgtgg
     7321 gcggcgacca cgatgagtat gccgtccacc agtcgagaca gcaccgccgc gtcgtacacg
     7381 ggagcgagcg ggggcgtatc gatgatgacg aagtcgtaga gggcgcgcgc ctcctggagg
     7441 aggatctcga atctgcgcga ccggatcagt tcagacacgt gagtcggcac cgcccccgcc
     7501 aggatgacgg cgacggtgct tccggggctg agcggccgga cgatctgccg cagctcgatg
     7561 tgttcgtcgc cgagcccctc ggccaggcca gcgcctttca cgccgtgaag accgagatgg
     7621 gcgccgacgg ccggccgccg cagatcgctg tcaatcagca ggacgcgggc gcctgcgccg
     7681 cgggccaggg cgacggccag gttgatcgac gtcacggtct tgccggcgct gatgtcgggg
     7741 ctggtgacgg ccagtacggt gacaccgcgc tcgtgtttca actgctcgat ccgcaggcgc
     7801 agcccttggt acttctcggc tgcgaaggtg ttcggagccg tcaggctgac gagcgcggcg
     7861 tcgatctgtc taggcatcat ctcttggcct ccatgatctc tgcgctatcc acctcgaacc
     7921 agcagtcgca cgagggacgc gttgtcgtgt gcgacgtacg cggagagcat tcccaggagc
     7981 acgaagcaga cgagcaccga ggcggtgagg agcgcgccgc gccgccagcg ggccgccccg
     8041 gacggcccga tgcggggaac ggccaccagc acgggaatct tggtgaaccg gcgcaggtcg
     8101 tccacgctgt ggaacgaagt gtcgaattga tcgaggagga gcaccacgag gccggccgca
     8161 agcgcggccg cgaagagggc catgatcaac aagcgcacgc gatttggccc gctgggcccc
     8221 gtcggaggca cggccgcttc catgatccgg aaccggtctc cctggctgga cgtctccatg
     8281 ttctcggcga gctgggccgc ctcgtactcc ttctggagcg atgcgtagtg ctcccgagcg
     8341 gcctggtgat cgcgggacat cgtcgcgaac tcattctggc gatggggcgc ccggtccagc
     8401 cgccgttcca ccatctcgat cgtctgacgg agtgaggctt cagcggcctt caacgatgcg
     8461 atctcggcgt ccaggctctc ggtcgtgcgg gcgccgcccg aaccccgtcc ggccgtgacg
     8521 gccgtgcgcg ccggtccggc cacagaggat ccggccgggc ccgtgccgcc ggccttctcc
     8581 agcgcggcga tctcgtcttt gattcgcctg acatccggat tccggtctgg gaagccgtcc
     8641 agctgcg
//
LOCUS       DBNGSH010000735         2608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3645608, whole genome shotgun sequence.
ACCESSION   DBNGSH010000735 DBNGSH010000000
VERSION     DBNGSH010000735.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3645608"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="AC16CY_16825"
     CDS             complement(<1..376)
                     /locus_tag="AC16CY_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355516.1"
                     /translation="MSDTHEVYAVRYAHHDRKAHENYIGGDPHDILQPLAYFVWVIKG
                     PHGTFVLDSGFDQSMADKRGRVLLKPVSTGLKAIDVDPDQVRDVIISHLHYDHCGNHD
                     LFPRARYHLQDREMAYGTGRFMC"
     gene            complement(369..1670)
                     /locus_tag="AC16CY_16830"
     CDS             complement(369..1670)
                     /locus_tag="AC16CY_16830"
                     /inference="COORDINATES: protein motif:HMM:NF012312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3355517.1"
                     /translation="MGTPLHAGARPMADAASALPWYRQVSREQWRAFWAVFIGWVVDS
                     FDFNILAFIIIDIQKSFTVDRALAGALGTVTLIMRLVGGTIAGTVADRWGRKLPLMLS
                     VLWFSLFAFLSGFAPSYAILFGIRALFGLGMGGEWAAGMPLVLEHWPARLRGLASGLL
                     LGGWYWGYLLSAAAFQFIYPLFSQTPDLAWRVMFWVCIVPALFTLWIRARVTESPVWL
                     ERQRRLKETGAAAREPKLSLVRIFQSDLVGTTIQTTLVIGSFMGIYYSLNFWYPTVLR
                     DAGRMNLLPFLAAFNIGAILGTATWGRLSEGRLGRRGAFTATLILGPASLPFFLYGGS
                     TTALVFGALTMGFFGMGVWGMAPAYTVERFPTEVRGVGPGFCYHAGSAVGAVMPTVLG
                     AMQDRGISAVTGMSAAMVTFAVLAGIAVWSGPETRGRDFNV"
     gene            1737..>2608
                     /locus_tag="AC16CY_16835"
     CDS             1737..>2608
                     /locus_tag="AC16CY_16835"
                     /inference="COORDINATES: protein motif:HMM:NF013604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355518.1"
                     /translation="MRIVMGAAVAAAALFVSLDVGTAQQAATLRFKATTGDELRTWDG
                     YVRTRERAGDLRVRQAVRDPSLPARTIERLQQYSNGVPVWGAEVVRDVERGVPLSIFG
                     ELSADLDIDPQPGMDTEAAERLLLSRQRSGAALLKRAALTIVRLATGEHRLAYTAVVS
                     AGSRVFRVFVDASSGAELLRYSSIHTQAEVGTGRGTLGDVRKMSVLRQGGVYIADDQH
                     RPPILTTFDMRGNLTRAINVVEQGAPLFVSDIASDSDNDWTDVATIDAHASIGLTYDY
                     YFKRHGRRGLDNQDR"
BASE COUNT          494 a          872 c          858 g          384 t
ORIGIN      
        1 ggcacatgaa gcgtcccgtg ccgtacgcca tctcgcggtc ctggaggtgg tagcgcgccc
       61 gcggaaacag gtcgtggttg ccgcaatggt cgtagtgcag gtggctgatg atgacgtctc
      121 tcacctgatc cgggtcgacg tcgatcgcct tgagaccagt cgagaccggt ttgaggagga
      181 cacgcccgcg cttgtcggcc atcgactggt cgaatcccga gtccagcacg aacgtaccgt
      241 gcggtccctt gatcacccag acgaagtaag ccagcggctg caggatgtcg tgcgggtcgc
      301 ccccgatgta gttctcgtgc gccttacggt cgtggtgcgc gtaccggacg gcgtagacct
      361 cgtgagtgtc agacattgaa atcgcgtccg cgcgtctcgg ggcccgacca gacggcgatg
      421 cccgcgagca cggcgaaggt caccatcgcc gcgctcatcc cggtgacagc tgatatgccg
      481 cggtcctgca tcgctccgag gacggtaggc atgacggcac ccaccgccga tccggcgtga
      541 tagcagaagc cgggaccaac tccgcggacc tcggtcggaa accgctcaac ggtgtacgcg
      601 ggcgccatcc cccagacgcc cattccgaag aagcccatcg tcagggcccc gaaaacgagt
      661 gccgtcgtgc tgccgccgta caggaagaac ggcagcgacg ccggaccgag aatcagcgtt
      721 gccgtaaacg cgccacggcg gcccagccgt ccttccgaca gccggcccca cgtcgccgtc
      781 ccgagtatcg cgccaatgtt gaacgcagcg aggaacggca gcaggttcat gcgtcccgca
      841 tcgcgcagca ccgtcggata ccagaagttc agcgaatagt agatgcccat gaacgacccg
      901 atgacgagcg tcgtttggat cgtcgtgccg accagatcgc tctggaagat gcgcacgagc
      961 gacagcttcg gttcgcgggc cgcagcgccg gtctctttca ggcgacgctg tcgctccagc
     1021 cacaccgggc tttcagtgac gcgcgcgcga atccacagcg tgaagagcgc ggggacgatg
     1081 cacacccaga acatcactcg ccaggcgaga tcaggtgtct ggctgaagag cgggtagatg
     1141 aactgaaagg ccgcggcgga cagcaggtag ccccaatacc agcccccgag cagcaatccg
     1201 gacgcgagcc cgcgaagtcg cgccggccag tgctcgagga cgagcggcat acccgcggcc
     1261 cactcgcccc ccatgccgag gccgaacaac gcacggatgc caaagaggat cgcatacgac
     1321 ggcgcaaatc cgctgaggaa cgcgaagagc gagaaccaga gcaccgacag catcaacggc
     1381 agcttccgtc cccatctgtc ggccaccgtt cccgcgatcg tgccgccgac caaccgcatg
     1441 atgagcgtca ccgttcccaa ggcgccggca agggcccggt ccacggtgaa gctcttctgg
     1501 atgtcgatga tgatgaaggc gaggatgttg aagtcgaacg agtcgacgac ccagccgatg
     1561 aacacggccc agaacgcccg ccactgctcg cgcgagactt gccggtacca cggaagcgcg
     1621 gaggccgcgt cggccatcgg ccgcgcgccg gcgtgcagag gggtgcccac gtggcgcgat
     1681 tctatgtgca cggccgggct ttgtcgacgc cggcttcctt tagaatcgga gccactgtga
     1741 gaatcgtcat gggggcggcc gtggccgcgg cggccctgtt cgtgtcgctc gacgtcggga
     1801 cggcgcagca ggccgcgacg ctccggttca aggcgacaac cggcgacgag ctccgaacgt
     1861 gggacggcta cgtccggaca cgggaacggg ccggcgacct gcgcgtcagg caggccgttc
     1921 gcgatccgtc cctcccggcg cgcacgatcg agcgcctaca gcagtattcc aacggcgtgc
     1981 ccgtgtgggg cgccgaagtg gtgcgcgacg tcgaacgcgg agtaccactc tcgatcttcg
     2041 gcgagctgtc ggcggatctc gacatcgacc cgcagcccgg aatggacaca gaggcagccg
     2101 agcggctgct cctgtcgcgc cagcggtcgg gtgcggcatt actgaagcgc gctgcgctca
     2161 ccatcgtcag gctcgcgacc ggcgagcatc gcctcgccta cacggcggtc gtctcggcag
     2221 gcagccgcgt attccgcgtg ttcgtcgatg caagctcggg cgcggagctc ctgcgatact
     2281 cctcgatcca cacgcaggcc gaggtcggca cgggccgtgg gacgcttggc gacgtgagga
     2341 agatgtcggt cctgcgccag gggggcgtct acatcgcgga cgatcaacac aggcccccga
     2401 tcctcacgac gttcgacatg cgaggcaacc tcacacgggc catcaatgtc gtcgagcaag
     2461 gcgcgccgtt atttgtctcc gacatcgcct ccgacagcga caacgactgg acggacgtgg
     2521 cgaccatcga cgcgcacgcg tcgatcgggt tgacctacga ctactacttc aagcggcacg
     2581 ggcgccgcgg gctggacaat caggaccg
//
LOCUS       DBNGSH010000736         3552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_759859, whole genome shotgun sequence.
ACCESSION   DBNGSH010000736 DBNGSH010000000
VERSION     DBNGSH010000736.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_759859"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..3147)
                     /locus_tag="AC16CY_16840"
     CDS             complement(166..3147)
                     /locus_tag="AC16CY_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355519.1"
                     /translation="MSRRILSCLLAVVGFGPIVVLSQAPNARPASPASRVTFTETIAP
                     IVYGNCVTCHRPGEAAPFSLISYEDVAKRGALIAKVTGSRYMPPWHAEPGFGEFAGER
                     RLTDGQIATIAAWVKQGMPRGDEAKMPKLPESSPDGWKLGTPDLILEMPAGFEVPASG
                     PDVFRNFVIPTRLTEDKWVRGIEFRPSARKVVHHAIFASVAGGSLAARDGADGRPGFG
                     GLSAVGITGQQSNGLGGWAVGATPRMLPPELTARLPKGSDFLLQMHFHPGGKVETEKS
                     LVGIYFADKGPDKDLMSVELPALFGFGAGIDIPPGEKRFTIRDSYTLPGDARVLMAIA
                     HAHYLARDMKAVATLPDGSTKGLLWISDWDFNWQDQYVYKQPFTLPKGTRIDVTLVYD
                     NSADNPRNPINPPRRALFGEYSSDEMGTVGFTFEVVNKPDVRSFEQTLGERNKLAIQA
                     AGKDGTLGRFLARGARQRRGLQQLTLFDRQGHVVSRLADAGAYSQAALSPDGSRVVAV
                     KSDADTDGQDLWVFDVASGRGTAITSDEPQDDAPIWSPDGSQIAYVSVRDNTRAIYRK
                     ASNGKGTEELLYRNTTGGTLNLTDWSADGRYLCFWAGESIFLLPLTGERTPIALDNER
                     LGRGGRFSPDGRLLAFSSNPSGRFQIYVKDLSGVTSPASASSAPERLQVSTDGGIGGI
                     FWRRDGRELYYLSQPPAQAIMAVEIASISPLRFGAPRALFQVPGPVGAPAQLSSVASP
                     DGQRFVFAVNVVPAPAAAPAAAPAAAPPSPAAAPVAAVAPGNGQARLDPQIVNDIFKG
                     FGGDVPALRRGLDASAKRLAEAPNDAEVLAWHGAAVLSLNRLGGDPAADFGASVKNFQ
                     LATGEMNQAVKLEPDNPLVRMARGVLLYIETPYMPRFANHPGLVENARADYQRLFDLK
                     KDQLQSLGTHRLGEILQGLGDLNSRQDKPDQAETYYTMIQSMLPGTEYAARASEWMKT
                     KKPLPTERTTCIGCHVGGQTP"
     gene            complement(3140..>3552)
                     /locus_tag="AC16CY_16845"
     CDS             complement(3140..>3552)
                     /locus_tag="AC16CY_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3355520.1"
                     /translation="CREYGPRGVGCSLIYEDVDVHPTSAHLDQQVRAHLREYSYAGVP
                     AAVDRDRTIATAAKATITPLAVVVDRSGDIRYRGRIDNLYAALGKRRQQVTKHDLREA
                     LDAVLSGSKVPNPETEALGCYITDPALLRKHDHE"
BASE COUNT          561 a         1185 c         1188 g          618 t
ORIGIN      
        1 gcgtcggaga tcgcacgacg ctcgactgga tgcatgagcc gtccggaccg caggatagcg
       61 gtccgtgacg gccggggcaa acgggttgca gcgaagggcc gcgatccggc ggcggccggc
      121 gtcacgcttg cagcgaagcg aagatccgct gcagtccaga actcttcagg gggtctgacc
      181 ccctacgtgg cagccgatgc acgtggtccg ctcggtcggg agcggtttct tcgtcttcat
      241 ccattccgac gctcgagcgg cgtattccgt gccgggaagc atcgactgga tcatcgtgta
      301 gtaggtctca gcctgatcgg gcttgtcctg ccggctgttg agatcgccaa gcccctgaag
      361 aatctcgccc agccggtgcg tcccgaggct ctggagctgg tccttcttca agtcgaacag
      421 ccgctggtaa tcggcgcgcg cgttctcgac gagcccaggg tggtttgcaa acctcggcat
      481 gtagggcgtc tcgatgtaca gaagaacccc ccgcgccatt cggaccaaag ggttgtcggg
      541 ctccagtttc acggcctggt tcatctcgcc ggtcgccagc tggaagttct tgaccgatgc
      601 gccgaagtcc gcggccgggt cgccccccag ccggttcagt gagagcacgg ccgcgccatg
      661 ccacgcgagc acctccgcat cgttcggtgc ttcagccagc cgttttgcgg aggcgtccaa
      721 cccgcgcctc agcgccggga cgtcgccgcc gaatcccttg aagatgtcgt tgacgatctg
      781 aggatcgagc cgggcttgtc cattcccggg cgccacggcc gcaacgggag ccgcggccgg
      841 agaaggcggc gccgccgcag gtgcggccgc cggcgctgcc gccggtgcgg gcaccacgtt
      901 gacggcgaag acgaagcgct gaccatcggg gctcgcgacg ctactgagct gcgccggcgc
      961 accgaccgga cccggcacct ggaagagggc gcgcggggct ccgaagcgca agggcgaaat
     1021 actcgcgatc tcgaccgcca tgatcgcctg cgcgggcggc tgtgacaggt agtacagctc
     1081 acggccgtcg cgccgccaga agattccgcc gatgccgccg tcggtcgaga cctgcagccg
     1141 ctccggcgcc gacgaggcgc tcgcggggct ggtgacgccg ctgaggtcct tcacgtagat
     1201 ctgaaagcgc cccgacgggt tggagctgaa cgcgagcagc ctgccgtcgg gcgagaaccg
     1261 tccgccacgc cccaggcgct cgttgtccag cgcgatcggc gtgcgctctc ccgtcagcgg
     1321 cagcaggaag atcgactcgc cggcccagaa gcacaggtat cggccgtcag ccgaccagtc
     1381 ggtgaggttg agcgttcccc ccgtcgtgtt ccggtacagc agttcttccg tccccttccc
     1441 gttcgatgct tttcgataga tcgctcgtgt gttgtcgcgt accgacacgt aggcgatctg
     1501 actgccgtca ggcgaccata ttggtgcatc gtcctggggt tcatccgacg tgattgccgt
     1561 tcctcttccg ctagcgacgt cgaacaccca gagatcctgt ccgtcggtgt cggcgtcgct
     1621 cttgaccgcg acgactcgag atccatcggg ggacaacgcg gcctgggaat acgcgcctgc
     1681 gtcggccaga cggctcacga cgtggccctg acggtcgaac agcgtgagct gctgcagccc
     1741 gcgccgctgc cgcgccccgc gcgccaggaa tcgcccgagc gtgccgtcct ttccggcggc
     1801 ttgtatcgcc agcttgttgc gttcccccag cgtctgctcg aacgagcgga cgtccggctt
     1861 gttgacgacc tcgaaggtga agccgacggt tcccatttcg tcggacgagt actcaccgaa
     1921 gagcgcgcgg cgcggtggat tgattgggtt gcgcgggttg tcggcggagt tgtcgtagac
     1981 gagcgtcacg tcgatgcgcg ttcccttggg taacgtgaat ggctgcttgt agacgtactg
     2041 gtcctgccag ttgaagtccc agtcggagat ccagagcagc cccttcgtcg agccatccgg
     2101 cagcgtggca acggccttca tgtccctggc cagatagtgc gcgtgcgcga tcgccatcag
     2161 gacgcgcgcg tctccgggca gcgtgtagga atcccgaatc gtgaaccgtt tctcgccggg
     2221 cgggatgtcg atgccggcgc cgaacccgaa gagcgcgggc agttcaaccg acatcagatc
     2281 cttgtcgggc cctttgtcgg caaagtagat gccgacgagc gacttctccg tttcgacctt
     2341 gccacccggg tggaagtgca tctggagaag gaagtcggac cccttcggaa gccgggccgt
     2401 caattccggc gggagcatcc tcggtgtcgc gcccactgcc cagccgccga ggccgttcga
     2461 ctgctgaccg gtgatgccga cggcgctgag gccgccaaag cccgggcgtc cgtcggcgcc
     2521 gtcgcgcgcc gccaggcttc cgcccgcaac ggacgcaaat atcgcgtgat ggacgacctt
     2581 ccgcgcgctc gggcggaact cgatgccccg gacccatttg tcctcagtca gcctggtggg
     2641 gatgacgaag ttccgaaaca catcaggccc gctggcaggc acctcgaaac ccgctggcat
     2701 ttcgaggatg agatcgggcg tcccgagctt ccatccatcc ggcgaggatt cggggagctt
     2761 cggcatcttc gcctcgtcgc cacggggcat gccctgcttg acccacgcgg cgatcgtcgc
     2821 gatctgcccg tcggtcagcc tgcgctcgcc ggcgaactcg ccaaatccgg gctccgcgtg
     2881 ccacggcggc atgtagcgag acccggtcac cttcgcgatg agcgcgccgc gctttgccac
     2941 gtcctcgtac gagatcaggg aaaagggcgc tgcctctccg gggcgatggc acgtgacgca
     3001 gttgccgtag acgatcggcg cgatcgtctc ggtgaaggtc acgcgcgacg ccggcgacgc
     3061 gggccgcgcg ttgggcgcct gcgaaaggac gacgatcggg ccgaagccaa caaccgcaag
     3121 gaggcaggac agaattcgtc tactcatggt cgtgttttct cagcaacgcg ggatcggtga
     3181 tgtagcaacc cagcgcctcg gtctccgggt tcggaacctt gctgcccgag agcacggcgt
     3241 cgagcgcctc gcgcaggtcg tgtttcgtca cctgctgccg ccgcttgccg agcgccgcat
     3301 acaggttgtc gattcgcccg cgatatcgaa tgtcgccgct gcggtcaacg accacggcca
     3361 gcggagtgat cgtggctttc gccgccgttg cgatggtccg atcgcgatcg acggccgccg
     3421 gcacgccggc gtagctgtac tcccggaggt gcgcgcgcac ctgctggtcc agatgcgcgc
     3481 tggtgggatg gacgtccacg tcctcgtaga tcagcgagca gccgacgccc cggggcccgt
     3541 actcgcggca ga
//
LOCUS       DBNGSH010000737         2475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3815291, whole genome shotgun sequence.
ACCESSION   DBNGSH010000737 DBNGSH010000000
VERSION     DBNGSH010000737.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3815291"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="AC16CY_16850"
     CDS             <1..873
                     /locus_tag="AC16CY_16850"
                     /inference="COORDINATES: protein motif:HMM:NF015922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR3355521.1"
                     /translation="IESEEEVAFTADDFIVEEDNVVIVSRDGWVKRQKEVRDLSTTRL
                     REGDAVLAVLQGSTRATVAFFTNLGAAYTSRIDDVPASTGYGEPVQKLFKLRDGEHVI
                     AAMSLDPRAAGEITPRSSKRRGGPSDPPADIPPVHAIAVTSDGYSLRFGLDSFVEPST
                     RAGRRYARPAGEAEVVGVARITGDEVIIAATAEARAILCRADEVNFLSGPGKGVILIK
                     LAAKDRVLGFLASTGDRDIMRVETSRGAEQTISTTKYEVTGRGGKGRELLQRGQFTRV
                     IPGEVENPLPLSPA"
     gene            complement(904..>2475)
                     /locus_tag="AC16CY_16855"
     CDS             complement(904..>2475)
                     /locus_tag="AC16CY_16855"
                     /inference="COORDINATES: protein motif:HMM:NF044955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HIR3355522.1"
                     /translation="VEVTETVVFRFVDGPFTFVFREIPRRGTDSIEVVRATMDGRALP
                     TGNQPGQVELGGNSRLRVQWHFAEPVSNSTHTFTLAYRVEGVVRQSEDGDLLAWRALP
                     QQHEYAIDSSTIEIELPAPLARPPAVESRRAAVPWTQFSASAADDRAQDQTVRLRASG
                     IGKNGWVEARLSFAPGSVIASPPLWQAARERADALAPSWLWAAALVAVAGFVLLWSLR
                     LRDDQPRRDTTFAGTSTAPPDSNPPAIAGALAANGHVSLANAMATLFSIADRGAIVIK
                     EEPRTLGQRTYSLDRRGARLSLAGHEQVALDLAFGGAQEQERQVSLSKARRRLQARLG
                     RFRDAVHTEMTAMGLVDARRKAVHTRFGAVSLGLLLLGAAGLFVVAVALVPQYRGWPL
                     MVPAAVIVVALAGFIFQGATTQLSDTGQRAGERWRAYQRHLKEVAREQAHLGAESPSG
                     VLPFAVALGLAAAWAKFAKTRPSLVPPWFQAEAASDAGGSFHAFVAYGGSPGGGHGGG
                     GGGGGAAGGGGSGAG"
BASE COUNT          420 a          870 c          833 g          352 t
ORIGIN      
        1 atcgaatcgg aggaagaggt cgccttcacc gccgacgact tcatcgtcga agaagacaac
       61 gtcgtgatcg tgtcgcgcga cggatgggtg aagcgacaga aggaagtccg cgatctctcg
      121 accacgcgtc tccgggaagg cgacgcggtg ctcgccgtgc tgcaggggag cacgcgcgcc
      181 accgtcgcgt tcttcacgaa cctcggcgcg gcctacacgt cgcggataga cgacgtgcct
      241 gcctcgacgg gctatggcga gccggtgcag aagctgttca agctgcgcga cggggagcac
      301 gtcatcgccg ccatgagtct ggatccacgc gcagccggcg agatcacgcc caggagctcg
      361 aagcgtagag gcgggccttc agacccgcct gcggacattc cccctgtcca cgccatcgcg
      421 gtgacgagcg atggctacag cctgcgcttc ggcctggatt cattcgtcga gccgagcacg
      481 cgcgccggcc gccggtatgc gcgcccggcc ggagaggccg aagtggtcgg cgtggcgcgg
      541 attaccggcg acgaggtcat catcgcggcg acggcggagg cgcgcgccat cctgtgccgg
      601 gccgacgagg tcaactttct ctccggtccc ggcaagggcg tgattctgat caagctggcg
      661 gccaaggacc gtgtgctcgg atttctggcg tcgaccggcg accgcgacat catgcgcgtc
      721 gaaacgagcc ggggcgccga gcaaacgatc agcacgacca agtacgaggt caccgggcgc
      781 ggcggcaagg ggcgcgagct gctgcaacgc ggacagttca cgcgcgtgat tccgggtgag
      841 gtcgagaatc cgttgccgct ctccccggcg tgaccggacc gatccctcac tcgcggcccg
      901 acatcagccg gcgccagacc ctccacctcc ggccgcgccg cccccgccgc ctcctccgcc
      961 atggccgccg ccgggagacc cgccgtacgc cacgaacgca tggaacgatc cgccggcatc
     1021 cgacgcggcc tctgcctgga accagggcgg cacgagcgat ggccgggtct tggcgaactt
     1081 cgcccacgcc gcagccaggc cgagcgcgac agcaaatggc agcacgccgc tcggcgactc
     1141 cgcgccgagg tgcgcctgtt cgcgagcgac ctccttcaag tgcctctgat acgctcgcca
     1201 ccgttcaccg gcacgctgtc cggtgtccga cagttgcgtc gttgccccct ggaagatgaa
     1261 tcccgccagc gcgaccacga tgacggcggc gggaaccatc aggggccagc cccgatactg
     1321 cggaaccagc gcgaccgcca cgacgaagag gccggcggcg ccaagcagga ggaggccgag
     1381 cgacacggcc ccgaatcgag tatgcacggc cttgcgcctg gcgtcgacga gacccatcgc
     1441 cgtcatttct gtgtgtacgg cgtcccggaa tcgcccgagg cgcgcctgca atcggcgtct
     1501 cgccttggac agcgacacct gccgctcctg ctcctgcgcg ccgccgaacg cgaggtcgag
     1561 cgccacctgc tcgtgacccg ccagtgacag cctggcgcca cggcgatcca gcgaatacgt
     1621 tcgctggccc aacgtccgag gttcttcctt gatgacaatc gcgcctctgt cggcgatcga
     1681 gaacagcgtg gccatggcgt tagccagcga cacatggccg ttggcggcga gcgccccggc
     1741 gatcgcggga ggattcgaat ctggaggcgc cgtgctcgtt cctgcaaacg tcgtgtcgcg
     1801 ccggggctgg tcgtcgcgca gccgcagcga ccacaacagg acgaaccccg ctacagcaac
     1861 gagcgcggca gcccagagcc atgaaggggc gagcgcatcg gcgcgctccc gcgcggcctg
     1921 ccagaggggc ggcgacgcga tgacgctgcc cggcgcgaag gacaaccgcg cctccaccca
     1981 gccgttcttg ccgatgccag aagcccgcag ccgtacggtt tggtcctgag cgcgatcatc
     2041 ggcggcactg gcgctgaact gtgtccacgg aacggcggca cgtctcgact cgacagccgg
     2101 cggccgcgcg agcggcgccg gaagctcgat ctcgatcgtg ctgctgtcga tcgcatactc
     2161 gtgctgctgg ggcagcgcgc gccaggcgag gaggtcgccg tcctcgctct ggcgcacgac
     2221 gccttcgacg cggtaggcga gcgtaaaggt gtgtgtcgag ttggagaccg gctcggcaaa
     2281 gtgccactgc actcgcagcc gcgagttgcc tccgagctcg acctgtccag gctggttccc
     2341 cgtcggcagc gcacgaccgt ccatcgtcgc gcgcacgacc tcgatcgaat cggtgcctcg
     2401 tctcgggatc tccctgaaca cgaacgtgaa cggaccgtcg acgaaacgga agacgacggt
     2461 ctcggtgact tcgac
//
LOCUS       DBNGSH010000738         5377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3137181, whole genome shotgun sequence.
ACCESSION   DBNGSH010000738 DBNGSH010000000
VERSION     DBNGSH010000738.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3137181"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC16CY_16860"
     CDS             complement(<1..226)
                     /locus_tag="AC16CY_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355523.1"
                     /translation="MNRTMDAAEELARTVSPFAERGHRRVYAPDRWLAAQVQQTIDPA
                     AVRLELWDGSSTYAGSRAPIGDLVVRDRRTL"
     gene            343..1116
                     /locus_tag="AC16CY_16865"
     CDS             343..1116
                     /locus_tag="AC16CY_16865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3355524.1"
                     /translation="MWIFVLAGFGVVALAHTWPARFVLDVLAGDRSVWRMPRNGSPTV
                     YLTYDDGPNPATTPDLLDVLAREEVPATFFLIDEHVTEDTAPLIRRMFAEGHSIGLHS
                     KSRSYMLMAPEDLASTLDAFAARIEGLGGAKPCRTFRPHAGWRSGEMYNGLKRGGYQL
                     IGWGWNRWDWNWFRTRTADVIVKRISRASAGDIIVMHDADEGAPDVDQRQTVDATARL
                     IPILRARGFAFGKVCENQTAWSSKNSAAPLSPTLQPSGA"
     gene            1056..2414
                     /gene="lysC"
                     /locus_tag="AC16CY_16870"
     CDS             1056..2414
                     /gene="lysC"
                     /locus_tag="AC16CY_16870"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HIR3355525.1"
                     /translation="MVVQKFGGTSVADPAAIRRLIDIVRAARSRDGRGPAVVVSAMSG
                     VTDALLGIATAAGESRADEALSKAEQIRARHLAAARELAAGPEQESLAGQIDAQFDQL
                     RDVIRTLAVLREVSPRMLDVVAAIGEILSSRIVAASLAGAGVPAEWVDARQVIVTSDD
                     HTRAVPLRRETDDRLRARLCPVLEAGGVPVLGGFVGATPDGHTSTLGRGGSDYSGALV
                     GAGIGASEIQIWTDVDGMLSADPRIVGSPRLVSQLSFAEAAELAYFGAKVLHPSTILP
                     AVERNIPVRILNSSKPGGAGTLITGAGLPRRTRLTGLASKRDVTVIDITSTRMLMAYG
                     FLRRVFEVFERFTTAVDMVTTSEVSVSVTVDDDRHIDAIVEALGEFSEVAVERHMSLL
                     CAVGDVLQDEPAMAAKVVGVLEEVPIRMISQAASRRNITVVLRQADLPHAMERLHEEF
                     FA"
     gene            2411..3073
                     /locus_tag="AC16CY_16875"
     CDS             2411..3073
                     /locus_tag="AC16CY_16875"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016726 - oxidoreductase activity, acting
                     on CH or CH2 groups, NAD or NADP as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3355526.1"
                     /translation="MRLLIVGYGKMGRMVEELAGGQGFDIAGRVDDGRPEWAAADVAI
                     DFSTADALLANFEQYVTRRLPVVIGTTGWNAHADRLRGDASRAGIGVVSSANFSIGVN
                     VFELVVAEAARMMQAQPQYGAWIHEIHHAAKRDAPSGTALLLRDAMTAAGFDRNIDMS
                     SARAGMIPGTHTVGFDSASDTIELTHRARDRRGFASGALLAARWIQGRSGWFTMQDVL
                     RV"
     gene            3094..3996
                     /gene="dapA"
                     /locus_tag="AC16CY_16880"
     CDS             3094..3996
                     /gene="dapA"
                     /locus_tag="AC16CY_16880"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3355527.1"
                     /translation="MRTPFTGVGTALITPFTRDGSLDESAVKRLARRQIDAGVHFLSP
                     CGTTGEAPTLTHRDKLRVVELVLEEAAGKVPVLAGAGGYDTREVVELARDMERVGADG
                     LLSVTPYYNKPTQEGLYQHFKAIAEATPLPIMLYNVPGRTSVNLSVETTLRLSEIRNI
                     VAIKEASGNVVQMSEIIAGAREDFMLLSGDDPLTVAVMAIGGRGVVSVASNEAPAEMS
                     QIVELCEKGDYAAARKLHHWLLPLIQVNFVETNPIPCKAAMAAMGLIEEHYRLPMVPP
                     GAAAREKIVRVLQQLKMLGAAARA"
     gene            4024..4866
                     /locus_tag="AC16CY_16885"
     CDS             4024..4866
                     /locus_tag="AC16CY_16885"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR3355528.1"
                     /translation="MSLSETISKLFAEGGAASRDAARQTFATLRAELASGRVRAAEPD
                     ASSPTGWRVNVWVKQGILLGFRFGDVVDMGIGKGRWPFFDKDTLPLKPLDGTSSVRLV
                     PGGSSVRDGAYLGKGIICMPPMYINVGAYVGDGTLVDSHALVGSCAQIGQRVHLSAAA
                     QIGGVIEPVGAMPVIVEDDVLVGGNCGIYEGAIVKRRAVLASGTIITGSTPVYDLPNG
                     RIIKPEPGQPLVIPEGAVVVPGARQVTVGAGREWGLSLATPVIVKYRDEKTDARTELE
                     RWIR"
     gene            4904..>5377
                     /locus_tag="AC16CY_16890"
     CDS             4904..>5377
                     /locus_tag="AC16CY_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3355529.1"
                     /translation="MPVDPVSLTRSLVDIESTTGREAAAGAWLSRFLQGLGYRVTEQP
                     VAGGRFNIFARLETAPAVVFSTHYDCVPPFFPSREERGLIFGRGACDAKGVLAAQVAA
                     AEALRQAGETAVALLFVVGEERGSDGARLANDQAPDGVRFLVNGEPTDNRLGAATR"
BASE COUNT          796 a         1778 c         1933 g          870 t
ORIGIN      
        1 ccagcgtgcg cctgtcgcgc accacgagat ccccgatcgg ggcacgcgat cccgcatagg
       61 tcgagcttcc gtcccacagc tccagcctca cagctgccgg atcgatcgtc tgttgtacct
      121 gcgcggcaag ccagcggtcc ggcgcgtaca cgcgcctgtg cccccgttcc gcaaacggac
      181 tgaccgtccg cgccagttct tcggccgcgt ccatagtccg gttcatgtcc atattcatac
      241 ttcaacctcg gcctgtcgat cacctgccgc ggggaggggg ccgtcccggt acactcttgc
      301 ggggccggaa cggagcgatg gcccggcact gagggggttt ttgtgtggat tttcgtgctg
      361 gccggtttcg gcgtcgtggc actggcacac acgtggcccg cccggttcgt gctcgacgtt
      421 ttggcaggcg atcgttccgt gtggcgcatg ccgcggaacg gatcgccgac ggtctacctg
      481 acctacgacg acgggcccaa ccccgcgacg acgcccgatc tgctggacgt gctggcccgc
      541 gaggaggtgc cggccacctt ctttctcatc gacgagcacg tcacggagga cacggcgccg
      601 ctcatccggc ggatgttcgc cgaggggcac agcatcgggc tgcattcgaa gagccggtcc
      661 tacatgctga tggcgccgga ggatctcgcg agcacgctcg acgcttttgc cgcgcgcatc
      721 gaaggcctcg gaggcgcgaa gccgtgccgc acgttccggc cccatgccgg gtggcgaagc
      781 ggcgagatgt acaacgggct gaagcgcggg ggatatcagc tgatcggctg gggctggaat
      841 cggtgggact ggaactggtt ccggacgcgc acggccgacg tcatcgtcaa gcggatctcg
      901 cgtgcgagtg ccggggacat catcgtgatg cacgacgccg acgagggggc gccggacgta
      961 gaccagcgcc agacggtcga tgccaccgcg cggctgattc ccattcttcg cgcgcgcggc
     1021 ttcgcgttcg gcaaggtgtg tgagaatcag acagcttggt cgtccaaaaa ttcggcggca
     1081 cctctgtcgc cgaccctgca gccatcaggc gcctgatcga catcgtgcgg gcagcgcggt
     1141 cgcgcgacgg ccgcgggccg gcggtcgtcg tctccgcgat gagcggcgtg accgacgcgc
     1201 ttctcggcat cgccacggct gccggtgagt cgcgcgcgga cgaggcgctg tcgaaggccg
     1261 agcagatccg tgcgcgtcac cttgcggccg cgcgtgagct cgcggccggc cccgaacagg
     1321 agtcgcttgc cggacagatc gacgcgcagt tcgatcaact gcgtgacgtc atccgcacgc
     1381 tggccgtcct gcgcgaggtg tcgccgcgca tgctcgacgt cgtggccgcg attggagaga
     1441 tcctctcctc ccgcatcgtt gccgcctcgc ttgccggcgc cggcgtcccg gccgagtggg
     1501 tcgatgcccg acaggtgatc gtcacgagcg acgaccacac gcgcgcggta cccctgaggc
     1561 gcgagaccga cgatcggctt cgcgcgcgat tgtgcccggt gctcgaggcc ggcggcgtgc
     1621 cggtcctcgg cgggttcgtg ggcgccacgc cggacggcca cacctcgacg ctcgggcgcg
     1681 gcgggtccga ttactccggc gcgctggtgg gggccggtat cggggcctcc gagattcaga
     1741 tctggaccga cgtggatggc atgctgagcg ccgatccccg catcgtcggg agcccgaggc
     1801 tcgtgtcgca gctctcgttt gcggaagcgg cggagctggc gtattttggg gccaaggtgc
     1861 tccacccgag cacgattctg cccgcggtgg aacggaacat tcccgttcgc atcctcaatt
     1921 cgtcgaagcc cggcggcgcc ggaaccctga tcacgggtgc ggggttgccc cgccggaccc
     1981 gtctcacggg gcttgcgtcc aagcgggacg tcacggtgat cgacatcacg tccacgcgga
     2041 tgttgatggc gtacgggttc ctgcggcggg tgttcgaagt gttcgagcga ttcacgacgg
     2101 cggtggacat ggtgacgaca tcggaggtga gcgtgtcggt gacggtggac gacgaccgtc
     2161 acatcgacgc gatcgtggag gcgctcggcg agttctccga agtcgcggtc gagcgccaca
     2221 tgtcgctgct ctgtgcggtt ggcgacgtgc tgcaggacga gccggcgatg gccgcgaagg
     2281 tcgtgggcgt gctcgaggag gtcccgattc ggatgatttc acaggcggcg tcgcgccgaa
     2341 acatcaccgt cgtgctgcgc caggcggatc tcccgcacgc catggagcgg cttcacgagg
     2401 agtttttcgc gtgaggctgc tcattgtcgg ctacgggaag atgggccgca tggtcgagga
     2461 gctcgccggc gggcaggggt tcgacatcgc aggccgcgtc gatgacggac ggcccgagtg
     2521 ggcggcggcg gacgtcgcga tcgacttctc gacggcggac gcgctgctcg ccaacttcga
     2581 gcagtacgtg acgcggcggc tgccggtggt gatcgggacc accgggtgga acgcccatgc
     2641 cgatcgcctg cgcggagacg cctcacgcgc gggcatcggc gtcgtctcgt cggccaactt
     2701 ctcgattggc gtgaacgtgt tcgagctcgt cgtggccgag gcagcgcgga tgatgcaggc
     2761 ccagccgcag tacggcgcgt ggattcacga gatccaccat gcggccaagc gggacgcgcc
     2821 gtcggggacg gcgctgctgc tgagagatgc gatgacggcc gccggattcg accggaacat
     2881 cgacatgtcg tccgccagag caggtatgat ccctggaacg cacacggtgg gtttcgacag
     2941 cgcgtcggat acgatcgagt tgacgcaccg cgcgcgcgat cgccgcggct ttgccagcgg
     3001 ggcgctgctg gcggctcgct ggatccaggg gcgatccggc tggttcacga tgcaggatgt
     3061 gctccgggtc tgaccccgtt caggagattt cccatgcgga ctccattcac aggcgttgga
     3121 acggcactga ttacgccgtt cacccgcgac ggctcgctcg acgagtcggc ggtgaagcgc
     3181 ctcgcgcgcc ggcagatcga cgccggcgtg cacttcctgt cgccgtgcgg gacgaccggc
     3241 gaggcgccca ccctgaccca ccgcgacaag ctgcgcgtcg tggagctggt gctcgaggaa
     3301 gccgccggca aggtgccggt gctggcgggc gcaggcggat acgacacgcg cgaggtcgtc
     3361 gagctggcgc gcgacatgga gcgggtcggc gccgacgggc tgctgtcggt gacgccgtac
     3421 tacaacaagc cgacgcagga aggcctctat cagcatttca aggcgattgc cgaggccacg
     3481 cccctgccga tcatgctcta caacgtgccg ggccggacga gcgtgaacct gtcggtcgag
     3541 accacgctcc ggttgtccga gatccgcaac atcgtggcga tcaaagaggc gtcgggcaac
     3601 gtggtgcaga tgtcggagat catcgccggc gcgcgcgagg acttcatgct gctcagcggc
     3661 gacgacccgc tcaccgtcgc cgtgatggcg attggcggcc ggggcgtcgt gtcggtcgcc
     3721 tccaacgagg cgcctgccga gatgtcgcag atcgtcgagc tctgcgagaa gggtgattac
     3781 gccgccgcgc gcaagctgca tcactggctg ctgccgctga tccaggtgaa cttcgtggag
     3841 accaacccca tcccctgcaa ggcggcgatg gcggcgatgg gcctcatcga ggagcactac
     3901 cggctgccga tggtgccgcc gggcgccgcc gcccgcgaga agatcgtgcg cgtgctgcag
     3961 cagctcaaga tgctcggcgc cgcggctcgc gcgtagttgt cgctcttcga cacgatcgcg
     4021 tagatgtcgc tctccgaaac gatctcaaag ctcttcgccg agggcggggc cgcctcgagg
     4081 gatgccgcgc gccagacgtt tgccaccttg cgcgccgagt tggcgtctgg ccgcgtccgg
     4141 gcggccgagc cggatgcatc gtcgccaacc ggctggcgcg tcaacgtgtg ggtgaagcag
     4201 ggcatcctgc tcggcttccg cttcggcgac gtcgtggaca tggggatcgg caaagggcgc
     4261 tggccgttct tcgacaagga cacgctgccg ctgaaaccgc tggacggcac gagcagcgtg
     4321 cgcctggtcc ctggcggctc gtccgttcgc gacggcgcct atctcggtaa gggcatcatc
     4381 tgtatgccgc cgatgtacat caacgtcggc gcctacgtcg gcgacggcac gctcgtcgat
     4441 tcccacgcgc tcgtcggctc gtgcgcgcag atcgggcagc gcgtgcacct gagcgcggcg
     4501 gcgcagatcg gcggcgtcat cgagccggtc ggcgccatgc ccgtcatcgt cgaagacgac
     4561 gtgctcgtcg gcggcaactg cggcatctac gagggagcga tcgtcaagcg gcgggccgtg
     4621 ctcgcctcgg gcaccatcat caccggctcg acgccggtgt acgacctgcc gaacgggcgc
     4681 atcatcaagc cggagccagg gcagccgctc gtgatccccg aaggcgcggt ggtcgtgccc
     4741 ggcgcgcggc aggtgaccgt cggcgcgggg cgcgagtggg gcctttcgct ggcgacgccc
     4801 gtgatcgtga agtaccgcga cgagaagacc gacgcccgca ccgagctcga acggtggatt
     4861 cgctgagcgc cgcacggccg gccgctcctc gcgacgagtg gacatgcctg tcgatcctgt
     4921 ttcactgacg cgcagcctgg ttgacatcga gtcaaccacc ggccgcgagg ccgcggccgg
     4981 cgcctggctg tcgcggttcc tccagggcct cgggtaccgc gtaaccgagc agccggttgc
     5041 gggcggccgc ttcaacatct tcgcgcggct ggagacggcg cccgccgtcg tgttctccac
     5101 gcactacgac tgcgtgccgc cgttcttccc gagccgggag gagcgcgggc tcatattcgg
     5161 ccgcggggcc tgcgacgcga agggcgtcct cgcggcgcag gtggcggcgg ccgaggcgct
     5221 gcgccaagcc ggcgagacgg ccgtcgcgct cctcttcgtg gtgggcgagg agcgcggcag
     5281 cgacggggcg cgcctcgcga acgaccaggc gccggacggc gtgcggtttc tcgtcaacgg
     5341 cgagccgacc gacaaccgcc ttggcgcggc gacccgc
//
LOCUS       DBNGSH010000739         2006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3476669, whole genome shotgun sequence.
ACCESSION   DBNGSH010000739 DBNGSH010000000
VERSION     DBNGSH010000739.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3476669"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..979)
                     /locus_tag="AC16CY_16895"
     CDS             complement(77..979)
                     /locus_tag="AC16CY_16895"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3355530.1"
                     /translation="MTPDPINLDDIAEIELFILRRMREYTLGDHASVFKGSGFNFVGV
                     RDWQPGDRMASIDWAQSSLNNFSPMVTREFEQDSNAAIVAVADASASTRCGIDGDLIA
                     TTVARAVAAAGLSALFFQDRFGLVTFDDRFEQVSAVRPRIGRSHLLYCLDLFRQPPLD
                     DRPTGSRDVIVAIESQVRKAALVPVISDFLLPDIERILAELSKLNAIHDVFLLMADAR
                     FAYELPGVSDGWVEVFDVESGRARVLSRREVGRLAHRTGAWQDEVMRLAKDADLDVVR
                     VGLDRWEMETALVEFAAERRLRKM"
     gene            complement(1056..>2006)
                     /locus_tag="AC16CY_16900"
     CDS             complement(1056..>2006)
                     /locus_tag="AC16CY_16900"
                     /inference="COORDINATES: protein motif:HMM:NF019346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3355531.1"
                     /translation="ALANVVLLSEIPTTYDKDQKRQVNAGNLLLRGVPGVGKTFFGVI
                     LAAISNAKFARLQGRADLQPTEIVGFQMINPATGEFITEFGPLASAEVILLDEINRIP
                     LKSQSAFLEGLQDRTVTVGKTTYEMAPFSFAIATMNPVELGQGTFPLSEAATDRFAIM
                     INIAYLRPQDEEKLVNFDFKQVRLKPLVPKERIIELRSVITREVFLHDSLAKYIRRLV
                     GATRPVSAETEWHHRSPSSLVEKCVDLGASPRATICLGRLVKVWALLLHRREEVYPED
                     IQELAKYVLSHRIWLGPHAASHGVTVEMVIDDIIATVPVP"
BASE COUNT          346 a          658 c          665 g          337 t
ORIGIN      
        1 gcggcggcgg acgacgcaag gactcccaac gtcacgatcg aaacggccag cgacgacgga
       61 aaccaggttc ttctcatcac attttccgga gccgtcgctc ggcggcaaac tccaccagcg
      121 ccgtctccat ctcccaacgg tcaagtccca cgcggacgac gtcgaggtcc gcgtccttcg
      181 ccagccgcat cacttcatcc tgccacgcgc ccgtacggtg cgccagccgc cccacttccc
      241 gcctcgaaag cacgcgggcc cggccgcttt cgacgtcgaa gacttcgacc cagccgtcgg
      301 agacgcctgg cagctcgtag gcgaagcggg cgtcggccat cagcaggaac acgtcgtgga
      361 tggcgttcag cttcgacagc tccgcgagaa tgcgctcgat gtcggggagc aggaagtccg
      421 agatcaccgg gacgagcgcg gccttgcgga cctggctttc gatcgccacg atgacatcgc
      481 gcgagcccgt ggggcgatcg tccaggggcg gctgcctgaa gaggtcgagg cagtagagca
      541 gatgcgaacg gccgatgcgg ggccggaccg ccgagacctg ctcgaagcgg tcgtcgaagg
      601 tcaccaggcc gaagcggtcc tggaagaaga gcgccgacag cccggcggcg gccaccgccc
      661 gggccacggt cgtcgcgatc aggtccccgt cgatcccgca gcgcgtggac gccgacgcgt
      721 cggccacggc cacgatggcg gcgttgctgt cctgctcgaa ctcgcgggtg accatcgggc
      781 tgaagttgtt gagcgacgac tgcgcccagt cgatcgacgc catgcgatcg ccgggttgcc
      841 agtcgcgcac gccgacgaag ttgaaccccg atcccttgaa cacgctggca tggtcgccaa
      901 gcgtgtactc ccgcatccgc ctgaggatga agagctcgat ctcggcgatg tcgtcgagat
      961 taatggggtc gggagtcatt tcttgaggac acttctgtga cggatcccga ggcggtggag
     1021 tgtcctcaag aaatgactcc cgaccccatt ttccgtcatg gcacgggcac cgtggcgatg
     1081 atgtcgtcga tgaccatctc gacggtcacg ccgtggctgg ccgcgtgcgg gccgagccag
     1141 atccgatggc tcagcacgta cttggccagc tcctgtatgt cctccgggta gacctcctcg
     1201 cggcggtgca gcagcagcgc ccacaccttg acgagtcggc cgaggcagat cgtcgccctg
     1261 ggagacgccc ccaggtccac gcacttctcg acaagcgacg acggcgaacg gtggtgccac
     1321 tcggtctccg cgctgacggg cctcgtcgcc cccaccagcc gccggatgta cttggcgagg
     1381 gagtcgtgca ggaacacctc gcgcgtgatg accgatcgca gctcgatgat gcgctccttc
     1441 gggacgagcg gcttgaggcg gacctgcttg aagtcgaagt tcacgagctt ctcttcgtcc
     1501 tggggccgca ggtacgcgat gttgatcatg atggcgaacc ggtcggtggc cgcctccgac
     1561 aagggaaagg tcccctgccc cagctccacg ggattcatcg tggcgatcgc gaagctgaaa
     1621 ggcgccatct cgtaggtcgt cttgccgacg gtgaccgtcc ggtcctgcag cccctcgagg
     1681 aacgcgctct gggacttgag ggggatgcgg ttgatctcgt cgagcaggat cacttcggcc
     1741 gacgcaagcg gcccgaactc ggtgatgaac tcgccggtgg ccgggttgat catctggaac
     1801 ccgacgatct ctgtgggctg caggtcggcc cggccctgga gcctcgcgaa cttggcgttg
     1861 ctgatcgccg cgaggatcac gccgaaaaac gtcttgccaa cgcccggaac gcctcgtagc
     1921 aggaggttgc cggcgttgac ctgccgcttc tggtccttgt cgtaggtggt cgggatttcc
     1981 gagagcagga cgacgttggc gagcgc
//
LOCUS       DBNGSH010000740         7262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_930086, whole genome shotgun sequence.
ACCESSION   DBNGSH010000740 DBNGSH010000000
VERSION     DBNGSH010000740.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_930086"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /gene="larE"
                     /locus_tag="AC16CY_16905"
     CDS             complement(<1..506)
                     /gene="larE"
                     /locus_tag="AC16CY_16905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR3355532.1"
                     /translation="MTTISTTAMTTSRTRTTTSDNLAAKERRLRNLLTGYQSLIVAFS
                     GGADSAYLAWVATDVLGLSVLCITADSPSYADRHRQLALAVARDAGLRHEIIQTSELE
                     RTEYRVNPANRCYYCKHELYTALTRIARARGFAEIADGSNADDRGDYRPGRQAAREFG
                     IRSPLDEAG"
     gene            complement(448..699)
                     /locus_tag="AC16CY_16910"
     CDS             complement(448..699)
                     /locus_tag="AC16CY_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355533.1"
                     /translation="MATCPECEFDEIDTEDLEEGDRLSCPECGKNLVVVGNDELDVAD
                     DEDDDEDLDDDDLDEEDDDDDDDDLDDGDDDVEDEDYDE"
     gene            847..1566
                     /locus_tag="AC16CY_16915"
     CDS             847..1566
                     /locus_tag="AC16CY_16915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3355534.1"
                     /translation="MVASTSLGSRTVLVADDTAFVRDRFKSAIEDAGHTAVTARTGSE
                     LLARLRTNPSKIDLVVLDLRLPQGNGVAMLRAIEKLEHHPAIVVFSGTIASAEEVRQL
                     DGLGVVGYVNEYTAVPHIVPSLVPHLFPDSLNRRSSPRVSLVIRVSYRFGNTIAAALT
                     LNISHGGLGIRTTSPLPVDTPVKVRFRLAGSRNEIDADARVAWIDRRIGMGLQFTRVT
                     PGDQTLIDSYVKSHFFSNRKA"
     gene            complement(1655..3784)
                     /locus_tag="AC16CY_16920"
     CDS             complement(1655..3784)
                     /locus_tag="AC16CY_16920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3355535.1"
                     /translation="MASPSSPVCTKCEIHAEAVFRVEGMDCNEEVVILERRLRPLAGM
                     EALSADLIGQRLHVTYDAAKLSTAAIVDAVGQTGMRMWLEHEAPAASGPDVRLRFWLT
                     ALSGALLVAGLAVGARYATGGAVLLVGASLAGAVFPVRRGLAALRSRTVDINVLMIVA
                     VAGAMALQQWLEAASVVFLFALAQWLEARTLERARQAIQALVELSPREAIVKRHGVEQ
                     RMPVDAVQVGMEIVVRPGDKVPLDGRVLTGHGDVNEAPITGESLPVDKGPGDEVYAGT
                     INGRGSLELEVIRVGTDTRLAHIIHLVEAAQARRAPVQNFVDRFARIYTPAVIALAAV
                     IAVVPPLLSGGDSADWFYRSLVLLVVACPCALVISTPVSIVAALSAAARNGVLIKGGA
                     HLERLADVRVVAFDKTGTLTRGEPRVIEVVPTGTHGADEVLRCAAAVESRSGHPIAGA
                     ITARAAEIGLVVPPATDFVSTTGRGAEARVDGTHVVVGSDSMFEAIGIDVAAEAVEAL
                     RRRGNTVVCVAADRRMLGAIAVADPLRDTARETIEMLRSQGIRRVAMLTGDHASTARA
                     VASSLSLDEDHHGLLPEEKHARIQAMRKTGEAVLMVGDGVNDAPALAAADVGVAMGAA
                     ASDVALETADVALMSGELLKLPYALRLARATVRNVKTNLIVSLVLKAVFLVAAMTGSA
                     TLWMAVLADTGASVIVVANALRLLRAR"
     gene            complement(3784..4095)
                     /locus_tag="AC16CY_16925"
     CDS             complement(3784..4095)
                     /locus_tag="AC16CY_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3355536.1"
                     /translation="MSQLDAHTVDGLTEIFRVLGDPTRVRILDALSRSEMCVGDLAQR
                     LGVSESAVSHQLRLLRNTRIVRSRREGRMIFYALDDRHVLALFRQGLRHVQEEGRQAG
                     R"
     gene            4211..5182
                     /locus_tag="AC16CY_16930"
     CDS             4211..5182
                     /locus_tag="AC16CY_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355537.1"
                     /translation="MIEVQHLTKRYGPLTAVDDVSFKAERGEILGFLGPNGAGKTTTM
                     RVLTGYMPPTDGKAIVAGYDVLEQPIEAKQRTGYLPETPPLYPDMTVADYLTFCARIK
                     GVPRSERASRIQTSMERTKIVDVAKRHCGKLSKGYRQRVGLAQALLHNPEVLILDEPT
                     AGLDPKQIIETRRLIKELGGDHTIILSTHILPEVSQTCNRIVIINRGRVVAVDTPDNL
                     TARLRGSETMYVQLDAQGADATGALTSVPGVTRVTASDKRDHVTGYEVESETGRDVRR
                     ELAAAVVGRGWGLLELRPMRMSLEEIFLQVTTEDAASPEGAVEASHE"
     gene            5175..5939
                     /locus_tag="AC16CY_16935"
     CDS             5175..5939
                     /locus_tag="AC16CY_16935"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355538.1"
                     /translation="MNNVLAIAGKELKGYFASPIAYVVIGLYAIPFGYFFYTLLLFFD
                     RQSMQMAGLAGPSVNVNEQLLRPVFLNTTVIFLFMLPMITMRTYSEEKRSGTIELLLT
                     APLTDFQIIAGKFLGAMGLYAAMLAITVPQVGLLFWFGNPEWIPIAITYLGLLLMGGC
                     FISVGLFISSLTKNQILAVMATFTVFLMLWVINWIATFTGPTTQSVLNYLSITDHLDD
                     FTRGVIDTKHLVYYFSFIAFGLYLTLRSVDSERWRG"
     gene            5945..>7262
                     /locus_tag="AC16CY_16940"
     CDS             5945..>7262
                     /locus_tag="AC16CY_16940"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HIR3355539.1"
                     /translation="MAKRILGALSYVGMALVLGALAVRLLKPEWDRYAAYAAWAGLAL
                     VVVYTLGQWREIAAYFRHRNARYGAMAGLGVVVVLGILVAVNYLSNRRNTRWDLTANK
                     QYSLSEQTVKLLRGLDAPVKFLVFDKETEFDRFRPRLTSYAYESDNVAVEYIDADKRP
                     VQARQYNVETYGTVVIEYKGRTERVNSDTEQELTNGLIKVVTGAQKKVYFTQGHGEKN
                     TDSSERNGYKTISDVLGRDNYAVAKVVLAQSQDVPADASVLVIAGPTSDLLPPEVEML
                     KRYLARGGHLLVLLDPPEDKSGPMPVLEGLLKEWAIEAGSDVVVDVSGLGQLLGTDAS
                     VPVAAKYSQHPITNGFGVLTAYPLARSITARTEGANGRFPQTIVETSERSWAETNVTE
                     LRASGKVEMNPGDKAGPISIAAAVSATATDADKTAAKPEAEAAKTKE"
BASE COUNT         1310 a         2407 c         2350 g         1195 t
ORIGIN      
        1 ccggcttcgt cgagcggact ccggatacca aactcccgcg ccgcctgccg cccgggccgg
       61 tagtcgccgc gatcgtcggc gttactgcca tcggcgattt ccgcaaaacc gcgggcgcgc
      121 gcgattctgg tcaacgccgt gtagagctcg tgcttgcagt agtagcaacg gttcgcgggg
      181 ttcacccggt actcggtgcg ctcgagctcc gacgtctgga tgatctcgtg gcgcagcccg
      241 gcgtcgcgcg ccacggcgag cgcgagctgg cggtggcgat cggcgtaact ggggctgtcg
      301 gcggttatgc aaagaacgga aagcccgagc acatcggtcg caacccaggc aaggtaggca
      361 ctgtcggcgc cgccgctgaa ggcgacgatg agcgactgat agccggttag aaggttccga
      421 agccgccgtt ccttcgctgc caggttgtca ctcgtcgtag tcctcgtcct cgacgtcgtc
      481 atcgccgtcg tcgagatcgt cgtcatcgtc gtcgtcgtcc tcctcgtcca ggtcgtcgtc
      541 gtccagatcc tcgtcgtcat cctcgtcgtc ggccacgtcg agctcgtcat tgcccaccac
      601 gacgaggttc ttgccgcact ccggacaact caggcggtcg ccctcctcga ggtcctcggt
      661 gtcaatctcg tcgaactcgc actcagggca ggtcgccata caggctccat tgtccatcag
      721 gtgctcgaag atgagcgaat caggcccttc ctggccaaga ttttggatta tagcgaggac
      781 ccgcggattt gggcaatgaa ccctaacgat cgccggtggc cgtaccgata gacccgatag
      841 actctcttgg tagcttccac cagccttggg tctcgaaccg tactcgtcgc tgacgacacg
      901 gcattcgtgc gcgaccgctt caagtcggcc atcgaagacg ccggtcacac ggcagtcact
      961 gcacgaaccg gctcggagct gctcgctcgg cttcggacca acccttcgaa gatcgatctg
     1021 gtcgtgctcg acctgcggct tccgcaaggc aacggcgtgg cgatgcttcg cgcgatcgag
     1081 aagctcgagc atcatccggc gatcgtggtg ttcagcggaa ccatcgcgag cgccgaggaa
     1141 gtgcgccaac tcgacggcct gggcgtcgtc ggctacgtga acgagtacac ggctgtcccg
     1201 cacatcgtgc cttccctggt cccccacctc ttcccggaca gcttgaaccg gaggtcgagc
     1261 ccccgcgtct cgctggtcat cagggtttcg taccggttcg gcaacacgat tgcggcggcc
     1321 ctgacgctca acatcagcca cggcgggctg gggattcgga ccacgagccc gctccccgtg
     1381 gacacgccgg tgaaggtccg cttccgcctt gccggatcca ggaacgaaat cgacgccgac
     1441 gcccgcgttg catggatcga ccgtcgcatc gggatgggac tgcagttcac gcgagtgaca
     1501 cccggcgatc agaccctcat cgacagctac gtcaagtcgc acttcttctc gaaccgaaaa
     1561 gcgtagcggt aaatggggtc gggagttatt ttcttgaaga cacttacact gttgggattc
     1621 gagacgtcct caagaaaata actcccgacc ccatttaccg cgcccgcagc aggcgcagag
     1681 cgttggccac cacgataacg gaggcgcctg tgtcggcgag gacggccatc cagagcgtgg
     1741 ccgagcccgt catcgcggcg acgaggaaga ccgctttgag caccagcgac acgatgaggt
     1801 tggtcttgac gttgcggacc gtggcacgcg cgagccgcag cgcgtaagga agcttcagca
     1861 gctcgccgga catgagggcc acgtcggcgg tctcgagcgc cacgtccgat gcggccgcac
     1921 ccatcgcgac gccgacgtcc gccgccgcca gggcgggggc gtcgttgacg ccgtcgccga
     1981 ccatgaggac cgcctccccc gtctttcgca tcgcctggat tcgcgcgtgc ttctcttcgg
     2041 gtagcaggcc atggtggtcc tcatcgaggg acaggctcga ggcaacggcg cgcgcggtcg
     2101 atgcgtgatc gcccgtcagc atcgccacgc ggcggatacc ctgcgagcgg agcatctcga
     2161 tggtctcgcg cgcggtgtct ctcagcggat ccgccacggc gatggccccc agcatccgtc
     2221 gatccgcggc aacgcagacc acggtgttgc cgcggcgacg cagcgcctcg acggcctcgg
     2281 ccgcgacgtc gatgccgatg gcctcgaaca tggagtcgct gccgacgacg acgtgcgtgc
     2341 cgtcaacgcg agcctcggct ccccttccgg tggtggagac gaagtcggtc gccggcggga
     2401 caacgaggcc gatctcggcg gcgcgggcag tgatcgcccc tgcgattgga tgcccggacc
     2461 ggctctcgac ggcagccgcg cagcgcagca cttcgtcggc accgtgagtg ccggtcggca
     2521 cgacctcgat cacgcggggc tctcctcgcg tcaacgtgcc ggtcttgtcg aacgcgacca
     2581 cgcgcacatc ggcgagccgt tcgaggtgcg caccgccctt gatgagaacg ccgttcctgg
     2641 cggccgccga cagcgccgcg acgatcgaga ccggcgtcga gatgaccagg gcacatgggc
     2701 acgcgacaac gagcagcacg agcgagcgat agaaccagtc tgcggaatct ccacccgaca
     2761 ggagcggcgg cacgacggca atgacagccg ccagtgcaat gaccgccggg gtatagatcc
     2821 tggcgaagcg atcgacgaag ttctggaccg gcgctcgccg ggcctgggcc gcctcgacca
     2881 ggtggatgat gtgggccagc ctcgtatccg tgcctacgcg gatcacttcg agctccagcg
     2941 agccgcggcc gttgatggtg cccgcgtaca cttcgtcgcc cgggcccttg tctacgggca
     3001 gtgactcgcc cgtgatgggc gcttcgttta cgtcgccgtg cccggtgagc acgcggccgt
     3061 cgaggggaac cttgtccccg ggccgcacga cgatttccat ccccacctgg acggcgtcca
     3121 ccggcatacg ctgctccacg ccgtgtcgct tgacgatcgc ttcacggggc gagagctcga
     3181 cgagagcctg aatggcttgt cttgcccgct cgagcgtccg cgcctccagc cactgggcga
     3241 gggcgaagag aaacaccacg ctggcagctt cgagccactg ctgaagcgcc atcgcacccg
     3301 cgaccgcgac gatcatgagg acgttgatgt caaccgtccg gctccgtagg gccgcgaggc
     3361 cgcggcgcac gggaaacaca gcaccggcga gcgaggcccc aacgaggagc acggcgccgc
     3421 ccgtcgcgta tctggcgccc acggccaggc cggcaaccag gagcgccccc gaaagcgcgg
     3481 tcagccagaa ccgcagccgg acgtcggggc cggaggccgc cggtgcctcg tgctcgagcc
     3541 acatccgcat cccggtctgg ccaacggcgt cgacgatggc ggccgtagac agctttgcgg
     3601 cgtcatatgt cacgtgcagg cgctggccga tgaggtcggc cgacagcgcc tccatgccgg
     3661 ccagcggccg aagtcggcgc tcaaggatga cgacctcttc attgcagtcc atcccctcga
     3721 cccggaacac cgcttccgcg tggatttcac acttcgtgca gacaggtgaa gaggggcttg
     3781 ccatcagcgt ccggcctgcc ggccctcctc ctgcacgtgc cgcagaccct ggcggaacag
     3841 ggccaggaca tgccgatcgt cgagcgcgta gaagatcatc cggccctctc gccggctgcg
     3901 cacgatgcgc gtgttgcgaa gcagccgcag ctgatgagac acggccgatt cgctcacgcc
     3961 gagccgctgg gcgaggtcgc ccacgcacat ttccgagcgc gacagcgcgt cgaggatgcg
     4021 cacgcgcgtc gggtcgccga gcacccggaa gatctcggtc aggccgtcaa cggtatgggc
     4081 gtcgagctgt gacatctgaa tacttgttca tatattactg caatgggctt gcccagttgg
     4141 ccggccatcg gctaggatcg cccgctgggc aggctaaaac gcgcaggttc tacgagttaa
     4201 ggagcgagca gtgatcgagg tgcagcatct gacgaagcgg tacgggccgc tcacggccgt
     4261 ggacgacgtg agcttcaagg ccgaacgggg agaaattctg gggtttctgg gaccgaatgg
     4321 cgccggcaag acgacgacga tgcgggtgct gaccggctac atgccgccaa ccgacggcaa
     4381 ggcgatcgtc gccggctacg atgtgctcga gcagcccatc gaagccaagc agcgtaccgg
     4441 ctacctgccc gagacgcccc cgctctatcc ggacatgacc gtagccgatt acctgacctt
     4501 ctgcgcgcgg atcaagggcg tgccgcggtc ggagcgcgca tcccggattc agacttcgat
     4561 ggagcgcacg aagattgtcg acgtggccaa acggcactgc ggaaagctct ccaagggcta
     4621 ccggcagcgc gtcggccttg cgcaggcgct cctgcacaac cccgaagtgc tcatcctcga
     4681 cgagccgacg gccggcctcg acccgaagca gatcatcgag acacggcgtc tcatcaagga
     4741 actgggcggc gaccacacga tcatcctcag cacgcacatc cttcccgagg tcagccagac
     4801 ctgcaaccgg atcgtcatca tcaaccgcgg ccgggtcgtc gccgtcgata cgccggacaa
     4861 cctgaccgcg cgcctccgtg gatcggaaac gatgtacgtg cagctggatg cgcagggcgc
     4921 cgacgccacg ggggcgctga cctccgtgcc gggcgtcact cgcgtgaccg catccgacaa
     4981 gcgggatcac gtcacgggtt acgaggtcga aagcgaaacc ggccgcgacg tgcgccgcga
     5041 gctggccgcg gccgtcgtgg gacggggctg gggcctgctg gagctgagac cgatgcggat
     5101 gagcctcgag gagatcttcc tgcaagtcac caccgaagac gcggcgtcgc ccgaaggcgc
     5161 ggtggaggcc agccatgaat aacgttctgg ccatcgccgg caaggagctc aagggatact
     5221 tcgcttcgcc gattgcctac gtcgtgatcg ggctgtacgc cattccgttc ggctacttct
     5281 tctacacgct cctgctgttc ttcgaccggc agagcatgca gatggcgggg ctggcgggac
     5341 cgtcggtgaa cgtgaacgaa cagctcctca ggccggtatt cctcaacacg acggtcatct
     5401 tcctgttcat gctgcccatg atcacgatgc gcacctactc cgaggaaaag cgctcgggca
     5461 cgatcgagct gctgctgacg gcgccgctga ccgacttcca gatcatcgcc ggcaagttcc
     5521 tgggcgcgat ggggctgtat gccgcgatgc tggccatcac ggtgccgcaa gtcggactgc
     5581 tgttctggtt cggcaacccc gagtggattc cgatcgcgat cacctacctc ggcctgctgt
     5641 taatgggggg atgcttcatc tcggtcggcc tcttcatctc gagcctgacc aagaaccaga
     5701 tcctcgcggt catggccacg ttcacggtgt tcctgatgtt gtgggtgatc aactggatcg
     5761 ccacgttcac gggaccgaca acgcagtccg tgctgaacta cctgtcgatc accgatcacc
     5821 tcgacgactt cacgcgaggc gtgatcgaca ccaagcatct ggtctactac ttcagcttca
     5881 tcgccttcgg gctgtatctg acgttgaggt cggtcgattc cgaaaggtgg cgtgggtaaa
     5941 gctcatggca aagcgaattc ttggtgcgct cagctacgtc ggcatggctc tggtgctcgg
     6001 ggccctggcc gtcaggttgt tgaagccgga atgggatcga tatgcggcct acgcggcgtg
     6061 ggccggcctg gcgctcgtcg tggtgtacac cctcggccag tggcgcgaga tcgccgccta
     6121 cttccggcac cggaacgcgc ggtacggcgc aatggccggc ctgggcgtgg tggtcgtgct
     6181 tggcattctc gtggccgtca actacctgtc caatcgccgg aacacgcggt gggatctgac
     6241 ggcgaacaag cagtacagcc tgtccgagca gacggtgaag ctgctgcggg ggctcgacgc
     6301 acccgtcaag tttctcgtct tcgacaagga gacggagttc gaccggttcc gcccgcgcct
     6361 gacctcgtac gcctacgagt cggacaacgt cgccgtcgaa tacatcgacg ccgacaagcg
     6421 cccggtccag gcacggcagt acaacgtcga gacctacggc acggtcgtga tcgagtacaa
     6481 gggccggacc gagcgggtca actccgacac tgagcaggag ctgaccaacg gcctgatcaa
     6541 ggtcgtgacg ggtgcgcaga agaaggtcta cttcacccag ggacacggag agaagaacac
     6601 cgactcgagc gagcgcaacg gctacaaaac gatctcggac gtgctcgggc gcgacaacta
     6661 tgcggtcgcc aaggtcgtgc tcgcacagtc gcaggacgta ccagccgatg cctcggtgct
     6721 cgtcatcgcc ggcccgacga gcgacctgct ccccccggaa gtggaaatgc tgaagcgcta
     6781 cctggccagg ggcgggcacc tgctcgtcct gctggacccg ccggaggaca agagcgggcc
     6841 gatgcccgtg ctcgaaggcc tgctgaagga gtgggcgatc gaagccggca gcgacgtcgt
     6901 cgttgacgtc agcggcctgg gccagctgct cggtaccgat gcctcggtgc ccgtggccgc
     6961 caagtattcc cagcatccga tcaccaacgg gttcggcgtg ctgacggcgt atccgctggc
     7021 gcgctcgatc acggcacgca ccgaaggcgc caacgggcgc tttccacaga ccatcgtcga
     7081 gacgagcgag cgcagctggg ccgaaaccaa cgtcaccgag ctgcgtgcca gcggtaaggt
     7141 ggagatgaac ccgggcgaca aggccgggcc catctcgatc gccgccgccg tttccgcaac
     7201 agccacggac gcggacaaga cggccgcgaa acccgaagcg gaagcggcaa agacaaagga
     7261 gg
//
LOCUS       DBNGSH010000741         3199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1269115, whole genome shotgun sequence.
ACCESSION   DBNGSH010000741 DBNGSH010000000
VERSION     DBNGSH010000741.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1269115"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="AC16CY_16945"
     CDS             complement(<1..223)
                     /locus_tag="AC16CY_16945"
                     /inference="COORDINATES: protein motif:HMM:NF014464.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA];
                     GO_process: GO:0090071 - negative regulation of ribosome
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HIR3355540.1"
                     /translation="MADTRARGASRETKAGPTRPRLPKAVAAAIGAVRDKKAEDVVVL
                     DLRKTGAFTDFFVICTGGNPRQMTAIADSV"
     gene            complement(210..884)
                     /gene="nadD"
                     /locus_tag="AC16CY_16950"
     CDS             complement(210..884)
                     /gene="nadD"
                     /locus_tag="AC16CY_16950"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR3355541.1"
                     /translation="MSAARRLGILGGTFDPIHFGHIDAAEAARTSLHLDEVLLIPSHD
                     PPHRSSPPRVSAFHRFALVALAIEDRPAYRVSDMELERARPSLTAPTLRALHGEGWRP
                     SQLFFILGADAFAEIASWSEFPGVLDGAHFVVIARPGTTLEAVSARTPSLATRMQGAQ
                     AGVPGDGNTRIVLLEARTRDISSTTIRARLAGRQPIDDLVPAGVARHITAHHLYEAVG
                     GLHGGH"
     gene            complement(881..1966)
                     /gene="obgE"
                     /locus_tag="AC16CY_16955"
     CDS             complement(881..1966)
                     /gene="obgE"
                     /locus_tag="AC16CY_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR3355542.1"
                     /translation="MFVDEVDIHVIAGSGGNGCLSFRREKYVPRGGPDGGDGGAGGSV
                     YVVAVATKNTLIDFRFHPDFKARRGRHGQGANRTGQTAPDLEIGVPVGTLVHDKHAEG
                     LRLLADLSGEGDRVLVARGGRGGRGNARFVSSTNRAPRRIEPGEGGEEKFLHLELKLL
                     ADVGLVGFPNAGKSTLISRVSAAHPKIADYPFTTLTPNLGVVTLSDDRSFVVADVPGL
                     IEGAHEGHGLGDRFLRHVERTKVLIHLVDVSGASGRDPVEDFDTIQRELLLFDPAVAA
                     KPQIVAANKIDALDDPARLERLKRHVKAKKLQLIPVSGVRGDGLAELLEAAWRQIALA
                     RVARVEHEAPEPDAVDLITPARRRRDA"
     gene            complement(2049..2306)
                     /gene="rpmA"
                     /locus_tag="AC16CY_16960"
     CDS             complement(2049..2306)
                     /gene="rpmA"
                     /locus_tag="AC16CY_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR3355543.1"
                     /translation="MAHKKGQGSSRNGRDSNSQRLGVKRHDGNVVTGGSILVRQKGRR
                     FEPGLNVGLGKDHTLYAKVAGKVRFEDHGQHGRRISVIPVQ"
     gene            complement(2343..2657)
                     /gene="rplU"
                     /locus_tag="AC16CY_16965"
     CDS             complement(2343..2657)
                     /gene="rplU"
                     /locus_tag="AC16CY_16965"
                     /inference="COORDINATES: protein motif:HMM:NF013025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR3355544.1"
                     /translation="MYAIVQARGQQLKLTPGAVIEVAGTAGEPGAAFTFDQVLLVEGD
                     AGEIVAGSPYVANAKVVGVVDGEARGPKLRVFKKKRRKGFRKTRGHRSTFTRVRITEI
                     LT"
     gene            2811..3044
                     /locus_tag="AC16CY_16970"
     CDS             2811..3044
                     /locus_tag="AC16CY_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355545.1"
                     /translation="MAKRPRDPNQLAWQIVQEATGQARPEPKALDTRNPAAVALSKLG
                     ASKGGHARAAALSPAKRKAIARKAIAARWAKKR"
BASE COUNT          525 a         1069 c         1060 g          545 t
ORIGIN      
        1 gcaccgagtc cgcgatcgcc gtcatctgcc ggggattgcc tcccgtgcag atgacgaaga
       61 agtcggtgaa ggcgccggtc ttgcgcagat cgagcacgac gacgtcctcc gccttcttgt
      121 cgcggacggc gccgatggcc gcggccaccg ccttgggcaa gcgaggcctg gtcggtccgg
      181 ccttggtctc ccgactcgcc ccacgcgccc tagtgtccgc catgcaagcc gcctaccgct
      241 tcgtagagat ggtgggccgt gatgtgcctg gccacgcccg ccggcaccag gtcgtcgatg
      301 ggctgacgac ccgcaaggcg cgcgcggatt gtggtggagg agatatcgcg cgtgcgcgct
      361 tcgagaagaa cgattcgagt gttcccgtct ccggggaccc cggcctgcgc gccctgcatg
      421 cgcgtggcga gcgagggcgt gcgcgccgag accgcctcga gcgtggtgcc cggcctcgcg
      481 atcacgacga agtgggcgcc gtccagcact cccgggaact cgctccagct ggcaatttct
      541 gcaaacgcat cggcgccgag aatgaagaaa agctgcgaag gccgccaccc ctcgccgtgc
      601 agcgcgcgca gcgtcggggc cgtcagtgac gggcgtgcgc gttcgagctc catgtccgac
      661 acacgatacg cgggacggtc ctcgatagcc agagccacga gcgcgaagcg gtgaaaggcc
      721 gatactcgag gagggctgct gcggtgaggc ggatcgtgcg agggaatcag gaggacctcg
      781 tccaggtgca gcgacgtgcg cgccgcttcg gcggcatcga tgtggccgaa gtgaatcgga
      841 tcgaatgtgc cgccgagtat cccgagcctg cgagccgcgc tcatgcgtcg cgccgccggc
      901 gcgccggcgt gatcagatcg accgcgtctg gttcgggcgc ttcgtgttcg acgcgggcga
      961 ccctcgcgag ggcgatctgc cgccacgcgg cttcgagcag ctccgcaagg ccatcacccc
     1021 gcacgccgga tacaggaatc agctggagct tcttcgcctt cacgtgccgc ttcagccgtt
     1081 cgaggcgcgc cggatcgtcg agcgcgtcga tcttgttcgc cgcgacgatc tggggtttcg
     1141 cggcgaccgc cgggtcgaac agcagcagct cccgctggat cgtgtcgaag tcctccaccg
     1201 ggtctcggcc cgacgcgccc gagacgtcca ccaggtgaat gagcaccttc gtacgttcga
     1261 cgtgccggag gaaccggtcg ccgagaccgt gtccctcgtg tgcgccttcg atgagccccg
     1321 ggacgtccgc caccacgaag ctgcgatcgt cgctgagcgt cacgacaccc aggttcggcg
     1381 tgagcgtggt gaacgggtag tcggcgatct tggggtgggc cgccgacacg cgcgagatca
     1441 acgtcgattt gccggcgttg ggaaagccga ccaggccgac gtcggccagc agcttcagct
     1501 ccaggtggag gaacttctcc tcgccgccct cgccgggctc gatgcgtcgc ggcgccctgt
     1561 tggtggacga gacgaagcgc gcgttgccgc gcccgccgcg tccgccgcgc gcgacgagca
     1621 cgcgatcacc ttcccccgag aggtcggcga gcagccgcag gccctcggcg tgtttgtcgt
     1681 gcacgagcgt gccgaccggc actccgatct cgaggtccgg cgcggtctgt ccggtccggt
     1741 tcgcgccttg cccgtgccgg ccgcggcgcg ccttgaagtc tggatgaaag cggaagtcga
     1801 tgagcgtgtt cttcgtggct accgcgacga cgtagaccga gccgcccgcg cctccgtcgc
     1861 cgccgtcagg accgccacgc gggacgtact tctcgcggcg aaaactcagg cagccgttgc
     1921 cgccggatcc ggcgatgacg tggatgtcga cctcatcgac aaacataggg ctcagggccc
     1981 agggccaggg ctcaggtggt gcctgaggcc tgcgacagcc ctgaggcctg agccctgatt
     2041 cctgaggcct actgtacggg aatcaccgag atccgtcgcc cgtgctggcc gtggtcttcg
     2101 aagcgcacct taccggcaac cttggcatag agcgtgtgat ccttgccgag cccgacgttg
     2161 agcccgggct cgaagcggcg ccccttctgc ctgacgagga tcgacccgcc cgtcacgacg
     2221 ttcccatcgt gccgcttcac gccgagacgc tgcgagttgc tgtcgcgtcc gttgcgtgag
     2281 cttccttgac ctttcttatg agccataggt tgatccttac gccttacgcc ttgagccttg
     2341 actcaggtca gtatctctgt gatccggacg cgcgtgaacg tgctgcgatg tccgcgcgtc
     2401 ttgcgaaaac ccttgcgccg cttcttcttg aacacgcgga gcttcgggcc gcgcgcctcg
     2461 ccgtccacga cgccgacgac cttcgcgttg gccacgtagg gagagccggc gacgatctcg
     2521 cccgcgtccc cttccaccag aagaacctga tcgaaggtga acgccgctcc cggttcgccc
     2581 gccgttccag cgacttcgat gacggcgccg ggagtcagtt tgagttgttg gccacgggcc
     2641 tgaacaatcg cgtacacggg cacctcacag cacgggccgc cggtggcggc acaacctatt
     2701 gagactaaca gatccggcaa cgcgccggca aggcagcggg gcctcaggta atggtctagt
     2761 ttcgatttct cggttcatac ttgcccgtcc acgtatgcga gaatcgcggt atggcgaagc
     2821 gaccccgcga tcccaatcaa ctggcgtggc agatcgttca agaggcgact ggacaggcac
     2881 gaccagaacc caaggcactt gatacccgca accctgccgc tgtcgccctg tcgaaactcg
     2941 gcgcgtccaa gggcggacat gctcgcgctg cggcgctatc gcctgcgaag cgcaaggcga
     3001 ttgcacggaa ggccatcgcc gcacggtggg ccaagaagcg ctagttaaga tcgagatgtc
     3061 gttcggcgca ctctcttgag atcccaagat gtctgaccat agaacggacg tggccggttt
     3121 cgatttcagg attctgccta cgaccgtgtg ggccaagcgg gagtgaccgg aaagacgtgc
     3181 cgttaaaggt aacggtcca
//
LOCUS       DBNGSH010000742         3430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_591006, whole genome shotgun sequence.
ACCESSION   DBNGSH010000742 DBNGSH010000000
VERSION     DBNGSH010000742.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_591006"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1769
                     /locus_tag="AC16CY_16975"
     CDS             <1..1769
                     /locus_tag="AC16CY_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355546.1"
                     /translation="ANARVLTVFDRQGKVVTTVGERGVYFDPVFSPDRTRLAVIQADY
                     EAETRDLWVFDVATGRRTRITSNRTLAEEWVRTPIWSPDGRQLAYAALRDGYEGVYRA
                     AADGTGAEELLYRHPGANLDVQDWSLDGRYLSFSTSDVFGRALFGSALYVLPVAGDGA
                     RTPTEVVRVEFALRGGVFSHDGRFVSYDSDESGRNEVHVRPISLSSVPVAGPPQAVSD
                     NGGRFTMHSGWRRDGREFYYVAQNLAIMAVEVKADSTIELGRPTLLFRPSQAIPLDPG
                     RISVSRDGERIVIAVPHAPKLEQVIVFDRQGAVLQRLGEPGLYRNPTLSPDGRRVAVT
                     RVVPETGDWDIWRFDLSSGVGTPVTSDAAEDNWPIWSPDGSELAYQSEHGLFARIHRK
                     RWDGTGNEERVFQYTPGAHLEVTDWSTDGRFLTFQDGCWGVLYVVPLAGAQDAQERRA
                     MEWLRGEYQVAEARFSPDVRHIAYLSDEVEADEFRLYVGPFDPKRPDGRGSAMPVQVL
                     SDNILGMVSWRADGRELYYLTPNWEVMAVDITTMPTIQAGTPRLLFTLPGPLPGNPKQ
                     WKSVTPDGQRFVFVLNVPVTVP"
     gene            complement(1801..>3430)
                     /locus_tag="AC16CY_16980"
     CDS             complement(1801..>3430)
                     /locus_tag="AC16CY_16980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3355547.1"
                     /translation="VLWFTGLIVVLYASLHAQSTSSTAASFEIADVRPSAPSAITVMR
                     GGAPRAGRYELRTATMVDLIRTAYAVDADKVVGGPHWLELDRFDVVAKVPASATRESV
                     RPMLQALLAERFGLAVRQEVADIDGYALVRGTEPKLRASAGGPSTGCQQLLQTSPAPG
                     ATGVIPTFVMTCRSVTLSVFAETLRRAMGTPQPGPVADSTGLSGTFDIDLRFTPPQLA
                     AIAGGQGATIFEAVDRQLGLKLEARKIPTPSIIVGRVNRTPTPNAPEVATAFPAEAPT
                     EFEVATLKVAGPESRAGSQMLPNGRVSLSAIPLRILLAMAWELTGSGPSMIVGPKFLD
                     TARYDIVAQLTTTGPIDPQGVDPEALRLALRKLVIDRLRIKAHMEDRPADAYALVSSG
                     RHKLTPAADPNSRTRCTDNLSSGARGQATPVLTRSVTCQNMTMTELAARLQPLSLGFF
                     QTPVTDETKLDGRWNFTLGFSPEFVTQALARGIGPAAPPAAGGARGLEAPEPTGALTI
                     VQAVDRQLGLKLERRPGKGQVLVIDAIDEEPLEN"
BASE COUNT          577 a         1137 c         1154 g          562 t
ORIGIN      
        1 aggccaacgc ccgagtcctc accgtattcg accgccaagg gaaagtcgtg acaacggtgg
       61 gcgaacgggg cgtgtacttc gatcccgtgt tctccccaga ccgaacgcgc ctggcggtga
      121 tccaggccga ctatgaagcc gaaacacgag acctctgggt gttcgacgtc gcgacaggac
      181 ggcgcacgcg catcacctcc aaccggacgc tggctgagga atgggttcgc acaccgatct
      241 ggtcgccgga cggccgccag ctcgcctacg cggcgctgcg cgatgggtac gagggcgtgt
      301 accgagctgc cgcggacggg acgggagcgg aggagctcct gtaccggcat ccgggtgcga
      361 acctggacgt tcaagattgg tctctggacg ggagatacct gagcttctcg acgtccgacg
      421 tatttggccg cgcgctcttt ggcagcgcgc tctatgtgct gccggtcgcc ggggacgggg
      481 cgcgcacacc caccgaggtc gtgcgcgtcg agttcgccct gcggggtggg gtcttctctc
      541 acgatggtcg cttcgtgtcg tacgactcgg acgagtcggg gagaaacgag gtccatgtcc
      601 gcccgatctc tctctcttca gtgcccgtgg ccggaccgcc acaggccgtt tcggacaacg
      661 gcggccggtt cacgatgcat tcggggtggc ggagggacgg ccgggagttc tactacgtgg
      721 cccagaatct cgccatcatg gccgtcgagg tcaaggcaga ttccacgatc gagctcggac
      781 ggccgacact gctgtttcgc ccgtcgcagg cgatcccact cgaccccggc cgaatcagcg
      841 tgagtcgcga tggggagcgg atcgtgattg ccgtgcccca cgcaccgaag ctcgagcaag
      901 tcatagtgtt cgaccggcaa ggcgccgtgc tgcaacggtt gggggaaccc ggcttgtacc
      961 gcaaccccac gctatcgccg gacggcagac gggttgccgt gacgcgggtc gttcccgaaa
     1021 cgggcgattg ggacatctgg aggttcgacc tgagctcggg cgttggcacg cccgtcacga
     1081 gcgatgccgc agaggacaac tggccgatct ggtctccgga cggcagcgaa ctggcgtacc
     1141 aatctgagca cggcctgttc gcgcgcatcc atcgaaaacg gtgggacgga acgggcaacg
     1201 aggagcgcgt ctttcagtac acgcccggcg cgcacctgga ggtgaccgac tggtcaacgg
     1261 acggacgttt cctgacgttc caggacggct gttggggcgt cctatacgtc gtgccgctcg
     1321 ccggggcgca ggacgcccag gagcggcggg cgatggaatg gctccgtggc gagtaccagg
     1381 ttgcggaggc cagattctcg cccgatgtac gccacatcgc gtacttgtcc gacgaggtcg
     1441 aagccgatga attcaggctc tacgtgggcc cattcgaccc caagcggccg gacggacgcg
     1501 gaagcgccat gccggttcag gtgctgagtg acaacatttt gggcatggtg tcctggcgag
     1561 cggatggaag ggaactgtac tacctgacgc ccaactggga agtgatggcc gtggacatta
     1621 ccacgatgcc cacgatccag gccggtacgc caaggctgct cttcacgctg ccaggcccgc
     1681 tgcccgggaa cccgaagcag tggaagagcg tgacccctga cggccagcgc ttcgtgttcg
     1741 tgctcaacgt cccggtgacc gtaccctgaa cggcccgcac cgcagctctc ctagctcaga
     1801 tcagttctcc agcggctcct cgtcgatggc atcgatgacg agcacctgac ccttgcccgg
     1861 acggcgctcc agcttgagcc cgagttgccg atcgacggct tgaacgatgg tcagcgcgcc
     1921 ggttggctcg ggcgcctcca ggccccgcgc gccaccggcc gccggcggcg cagccggccc
     1981 gatgcccctg gccagcgcct gcgtcacaaa ctccggtgag aatcccaacg taaagttcca
     2041 ccggccgtcg agcttcgtct cgtcggtcac tggcgtctga aagaaaccaa ggctgagcgg
     2101 ctgcagtcgc gcggccagct cggtcatcgt catgttctgg caggtcacgc ttctggtgag
     2161 gacgggcgtt gcctgaccgc gtgcgcctga cgagaggtta tcggtgcagc gggtgcggct
     2221 gttcgggtcg gcggccggcg tcagcttgtg gcggcccgat gagacaagcg cgtaggcgtc
     2281 cgccggccga tcctccatgt gcgccttgat tctcagccga tcgatgacga gcttccgcag
     2341 cgccaggcgg agcgcttcgg ggtccacacc ctgtggatcg atcggaccgg tcgtcgtcaa
     2401 ctgcgccacg atgtcgtacc tggcggtatc gaggaacttc ggcccgacga tcatgctcgg
     2461 acccgaacct gtcagctccc acgccatggc caacagaatc cgcaacggga tcgccgacag
     2521 ggagacccgg ccgttgggca gcatctgcga gccggcgcga ctctccggcc ccgcgacttt
     2581 gagcgtggcc acctcgaact cggtcggtgc ctccgccgga aacgcggtcg ccacctccgg
     2641 cgcattcggc gtcggcgtgc gattgacgcg accgacgatg atcgacggcg tcgggatctt
     2701 cctggcctcg agcttgagac cgagctgtcg atcgaccgcc tcgaagatcg tggcgccctg
     2761 accgccagca atcgcggcca gttgcggcgg ggtgaatcgc aggtcgatat cgaacgtgcc
     2821 cgacaacccc gtcgagtcag cgaccggacc cggctgcggc gttcccatcg cgcggcgcag
     2881 cgtctccgcg aagacgctca gcgtgacgct ccgacaggtc atcacaaacg tcgggatcac
     2941 ccccgttgca cctggtgcgg gcgacgtctg cagcagctgc tgacagccgg tcgaggggcc
     3001 gcctgccgac gcccgcagct tcggctccgt cccgcgcacg agtgcatacc cgtcgatgtc
     3061 ggcgacctcc tggcggacgg ccagaccgaa gcgctccgcg agcagcgcct gcagcatcgg
     3121 cctcacgctc tcgcgcgtcg ccgatgccgg taccttagcg acgacatcga atcgatcgag
     3181 ttcgagccag tgcgggccgc cgacgacctt gtcggcatcg acggcgtacg cggtgcgaat
     3241 gagatccacc atcgtcgcgg tgcgcagttc gtagcggccg gcgcgcggag cgccgccgcg
     3301 catcaccgtg atcgcgctgg gcgcgctcgg tcggacgtcg gcaatctcga acgatgccgc
     3361 ggtggacgat gtggactgcg cgtgcagcga cgcgtacagg acgacgatca ggcccgtaaa
     3421 ccacaacacc
//
LOCUS       DBNGSH010000743         9028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_81354, whole genome shotgun sequence.
ACCESSION   DBNGSH010000743 DBNGSH010000000
VERSION     DBNGSH010000743.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_81354"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..363
                     /gene="rplX"
                     /locus_tag="AC16CY_16985"
     CDS             31..363
                     /gene="rplX"
                     /locus_tag="AC16CY_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3355548.1"
                     /translation="MAHLDTPIRRNDNVVVKTGKDRGKRGRVLKVLPATNRVVVEGVN
                     MIKRHTRPNPRANIKGGIVQREASLHASNVQLICPECGAQARLGRKVLGDGRKVRICR
                     KCEGVVDK"
     gene            360..902
                     /gene="rplE"
                     /locus_tag="AC16CY_16990"
     CDS             360..902
                     /gene="rplE"
                     /locus_tag="AC16CY_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3355549.1"
                     /translation="MSRLKDRYLKDVAPALKKEFGYRNVMAIPRLRKIVINMGLGEAT
                     SNAKLADVGADELGRITGQKAVVRRATKSIAQFKLRQGMPVGAMVTLRGERMYEFLDR
                     LITIALPRVRDFRGVSPKGFDGRGNFTMGLRDQLLFPEIDYMKVDKARGMNVSVVTSA
                     RTDEEARKLLQLMGMPFRAN"
     gene            944..1129
                     /locus_tag="AC16CY_16995"
     CDS             944..1129
                     /locus_tag="AC16CY_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR3355550.1"
                     /translation="MATTAKVAKEAKTPKYHVRHRNRCKLCGRPRAFMRKFDMCRLCF
                     RKLALEGDITGVTKSSW"
     gene            1193..1606
                     /gene="rpsH"
                     /locus_tag="AC16CY_17000"
     CDS             1193..1606
                     /gene="rpsH"
                     /locus_tag="AC16CY_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3355551.1"
                     /translation="MTDPISDMLTRLRNAVTAKHTRVDLPASKLKAEIARILQDEGYI
                     QGFRLVEEPATREGRQPRQVIRVFLKYGPHGEKVLTGLERISRPGRRVYLGVQDVPSV
                     LGGLGTSILTTSRGVMTGRAARKAGVGGEVLCNVW"
     gene            1614..2153
                     /gene="rplF"
                     /locus_tag="AC16CY_17005"
     CDS             1614..2153
                     /gene="rplF"
                     /locus_tag="AC16CY_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR3355552.1"
                     /translation="MSRIGKKPIPIPKGVTVKIAPDAVEVQGPKGKLRQAVPPGILFA
                     EDAGALVARLSVEDDELRKFQGLARSLVANAVAGVTEGFKKELDIVGVGYRAELKGKQ
                     VHFSLGYSHPVIFDIPAGIDIAVDKQTHIVVTGADRQLVGQVAANIRRMRKPDPYKQK
                     GVRYTGEQLKKKVGKTGAK"
     gene            2188..2562
                     /gene="rplR"
                     /locus_tag="AC16CY_17010"
     CDS             2188..2562
                     /gene="rplR"
                     /locus_tag="AC16CY_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR3355553.1"
                     /translation="MRIKTKEDRRDRIKYRIRRRVQGSPERPRLTVFRSVSHMYVQVV
                     DDTTGRTIASASTVEPAVKGGLPKKAGGGNIAGAKAIGKTIAERLLGQGLKRVVFDRN
                     GFLYHGRVKAVAEAAREAGLEF"
     gene            2573..3088
                     /gene="rpsE"
                     /locus_tag="AC16CY_17015"
     CDS             2573..3088
                     /gene="rpsE"
                     /locus_tag="AC16CY_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR3355554.1"
                     /translation="MNREREKIDASQLEIKDTVVSINRVTKVVKGGKNLSFSALVVVG
                     DAHGVVGFGVGKAKEVPSAIKKGIEAAKKNLIRVPLKGTTIPHQVVGRFGSGSVLLKP
                     APEGTGLIAGGAVRAVVESAGIQNVLTKSLGSANPHNVVRATFAGLMGLKDPGQVARF
                     RGKELAELVGA"
     gene            3089..3295
                     /gene="rpmD"
                     /locus_tag="AC16CY_17020"
     CDS             3089..3295
                     /gene="rpmD"
                     /locus_tag="AC16CY_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR3355555.1"
                     /translation="MAKKAAKKTSAKRLKITLVKSTIGFNRTQDDTVTGLGLRRLRHT
                     VELPDTPETRGMIHKVRHLVVVQE"
     gene            3300..3785
                     /gene="rplO"
                     /locus_tag="AC16CY_17025"
     CDS             3300..3785
                     /gene="rplO"
                     /locus_tag="AC16CY_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR3355556.1"
                     /translation="MDLSDLKPAKGSKKNRKRVGRGHGTGQGTQAGRGHKGAQSRSGF
                     KFKRGFEGGQMPLHRRIPKRGFHNPFRVEYVVVNLDTLAERFESGTVVTPELMRAKGL
                     VSSARGLIKVLARGEVSKKLTVRAHKFSGKAAEKIAAAGGAAEVIATVTAAVGKDGER
                     A"
     gene            3769..5157
                     /gene="secY"
                     /locus_tag="AC16CY_17030"
     CDS             3769..5157
                     /gene="secY"
                     /locus_tag="AC16CY_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3355557.1"
                     /translation="MENALKNLFAVTDLRNRVLFTLALLGVYRIGARVPTPGVNQEAL
                     RLFADQLQGSMFGLANMFTGGSLERVTIFALGVMPYISASIIIQLLTVVWPYLERISK
                     EGELGRRKITQYTRYLTVVLAVVQSFGYAVWLESTSDAPGGLPLVFDPGWGFRLMCVL
                     TLTTGTMFVMWLGEQITERGIGNGMSLLIFAGIVVGLPTALLATLDQLQTQQITLLRV
                     AFLVVMAVVVIGVIVFVERAHRRITVQYAKRVVGRRQYGGSSTHIPLKVNTGGVIPVI
                     FASSILSFFTMAAPLFEVGTWARRALDQVAYGMPLYNLLYVVGIIFFAYFYTSIIFNP
                     DDVAENMRKYGGFVPGIRPGKRTAEYIDTILARITLVGALYLALVALLPELLISGFRV
                     APIPYIGETLDAILPPWFTQGLGVTFFFGGTSLLIVVGVAMDTVQQVESQLIMRHYDG
                     FMKKTRIRGRRG"
     gene            5176..5832
                     /locus_tag="AC16CY_17035"
     CDS             5176..5832
                     /locus_tag="AC16CY_17035"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR3355558.1"
                     /translation="MGLNVVVMGPPGAGKGTQAGRFARDRGLPRIATGDILREGVKEK
                     NPIALEARARMDRGELVDDATMIKIVADRLLRPDVDAGFVLDGFPRTVAQARALDELI
                     GQRGRGPLIVVDLAVPDEEIVRRLAGRRICSECGTNEDPQYPGDRCRNCGGRLVQRGD
                     DDRKVVQERLRVYQQSTRPLLDYYRDRPTFRAVNGAQPPELVARELDAMIDAAAAARR
                     "
     gene            5843..6685
                     /gene="map"
                     /locus_tag="AC16CY_17040"
     CDS             5843..6685
                     /gene="map"
                     /locus_tag="AC16CY_17040"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3355559.1"
                     /translation="MIVCRSKSEIDKLRRVNQLVARILAELRRMVTPGVTTEAIDLFA
                     ERQVRDAGAEPAFKGYHDYPATVCASINEQVVHGIPSSRALVSGDIVSIDMGARLDGF
                     FGDCAVTVPVGHVTPDAARLLQVTEEALFRGIDCVRPGARVSDIGAAVQQHVEAQGYS
                     VVREFVGHGIGTELHEEPQVANYGPAGRGPRLSEGMVLAIEPMVNAGLPGVKILSDRW
                     TAVTSDRSLSAHFEHTVVVTRDGVEILTLLPEDEARARQLLAAARSPNGQGRDESEGY
                     PART"
     gene            6743..6922
                     /locus_tag="AC16CY_17045"
     CDS             6743..6922
                     /locus_tag="AC16CY_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing protein"
                     /protein_id="HIR3355560.1"
                     /translation="MTAQLPSGLYQVEVGGEQRLTAHPASGTERNFVRLLVGDRVVVE
                     VTARDGTRGRIVRKA"
     gene            6925..7038
                     /gene="rpmJ"
                     /locus_tag="AC16CY_17050"
     CDS             6925..7038
                     /gene="rpmJ"
                     /locus_tag="AC16CY_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR3355561.1"
                     /translation="MKVRASVKRICDKCKVVRRRGVVRVICTNAKHKQRQG"
     gene            7043..7420
                     /gene="rpsM"
                     /locus_tag="AC16CY_17055"
     CDS             7043..7420
                     /gene="rpsM"
                     /locus_tag="AC16CY_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR3355562.1"
                     /translation="MARIAGIDLPRTKRVEIGLTYIYGIGRKRSGDILGAAGVSPDVR
                     VKDLSEDDVRKISRVIEEVGGVEGDLRKEVSMNIKRLMEIGCYRGLRHRRNLPARGQR
                     TRTNARTRKGPRKGAVAKKKTTV"
     gene            7430..7867
                     /gene="rpsK"
                     /locus_tag="AC16CY_17060"
     CDS             7430..7867
                     /gene="rpsK"
                     /locus_tag="AC16CY_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR3355563.1"
                     /translation="MAKTEAAPDKKAEGVKKAPRKTFKKRGEKRVVHHGLVHIHASFN
                     NTQITITDTEGNVIAWSSAGGIGFKGSRKGTPFAATQAAMSAGNTAKTYGLRSVDVRV
                     KGPGSGRESAIRALQTVGIEVKSIRDVTPIPHNGCRPTKRRRV"
     gene            7902..8531
                     /gene="rpsD"
                     /locus_tag="AC16CY_17065"
     CDS             7902..8531
                     /gene="rpsD"
                     /locus_tag="AC16CY_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3355564.1"
                     /translation="MARYIGPVCRLCRREGMKLFLKGERCYTEKCAIEKRNFAPGQHG
                     KTRKSKLAGYGVQLREKQKVKRIYGVLEDQFRRYFEQAERTRGITGETLLQLLERRLD
                     NVAYRLGFATSRPQARQLVRHGHFTVNGKKVDIPSFSVKPGDVVAVRQSSRKSAAILH
                     ALEEVKGRGVPEWLQFDAEGLSAKIGSVPTREQINLPVQEQLIVELYSK"
     gene            8667..>9028
                     /locus_tag="AC16CY_17070"
     CDS             8667..>9028
                     /locus_tag="AC16CY_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3355565.1"
                     /translation="MLWKGFQRPKRLEFERETLTDRFGRFYAQPFERGFGTTIGNALR
                     RVLLSSIEGAAITAVKIDGVLHEFSPIPGVVEDATDIILNLKQIPLKMHVDTTKTLYL
                     RVDKPGEVKARDIETDAD"
BASE COUNT         1668 a         2716 c         3223 g         1421 t
ORIGIN      
        1 tgactgctga ctgctgactg ctgacttgct atggctcatc tagatacccc gattcgccgc
       61 aacgacaacg tggtcgtcaa gacgggcaag gaccgcggca agcgtgggcg agtcctgaaa
      121 gtgctgccgg cgacgaaccg tgttgtggtc gaaggcgtga acatgatcaa gcgccacacg
      181 cgtcccaacc cacgcgccaa tatcaagggt gggattgtgc agcgcgaggc gtcgctccac
      241 gcgtcgaacg tgcaactcat ctgtcccgag tgcggcgctc aggcgagact cggccgaaag
      301 gtgcttggcg acgggcgcaa ggttcgcatc tgccgcaagt gcgaaggggt ggtggacaaa
      361 tgagccgcct gaaggatcgg tatctgaagg acgtcgcgcc ggcgctcaag aaggagtttg
      421 gctaccgcaa cgtgatggcg atcccccggc tgcggaagat cgtcatcaac atggggctgg
      481 gcgaggccac cagcaacgcc aagctggccg atgtcggcgc cgacgagctg ggccggatca
      541 ccggacagaa ggccgtcgtc aggcgcgcga cgaagtcgat cgcccagttc aagctgcggc
      601 aggggatgcc cgtcggcgcc atggtcacgc ttcgcgggga acgcatgtac gagttcctcg
      661 atcggctgat cacgatcgcg ctcccgcgcg tgcgcgactt ccgcggcgtg tcgcccaagg
      721 ggttcgacgg gcgcggcaac ttcacgatgg ggctccgtga tcagctgttg tttcccgaga
      781 tcgactacat gaaggtcgac aaggccaggg gcatgaacgt ctcggtggtg accagcgcgc
      841 ggaccgacga agaggcgaga aagctccttc agttgatggg gatgccgttc agagcgaatt
      901 aataggcctg gggcctgagc cctgaggcct gcgaggatcg agtatggcca cgaccgcgaa
      961 agtcgccaag gaagcgaaaa cgccgaagta ccacgttcgt caccggaacc gctgcaagct
     1021 gtgcggacgg ccgcgagcgt tcatgcgcaa gttcgacatg tgccgtctgt gcttccgcaa
     1081 gctggcactc gaaggggaca tcacgggtgt gacgaagagt tcttggtagt agggagacgc
     1141 ggcaggctgt gaactgccta ctgcctactg tgaacgagca gggaacgaag agatgacaga
     1201 tccgatttcg gacatgctga cgcgtttacg gaacgccgtg acggccaagc acacgcgggt
     1261 cgatcttccg gcgagcaagc tgaaggcgga gatcgcgcgg atcctccagg acgagggcta
     1321 catccagggc tttcgtctcg tcgaggagcc ggcgacgcgc gagggacggc agccccggca
     1381 ggtcattcgc gtgttcttga agtacggccc ccacggcgaa aaggtcctca ccgggctcga
     1441 gcgcatcagc cgccccggcc ggcgcgtcta cctcggcgtg caggacgtgc cgagcgtact
     1501 cggcggactc ggcacgagca tcctgacgac gtcgcggggc gtgatgaccg ggcgcgcggc
     1561 gcgcaaggcc ggcgtgggcg gcgaagtgct ttgtaacgtc tggtagggca gtcatgtcgc
     1621 gaatcggtaa gaagccaatc ccgatcccca aaggggtcac cgtgaagatc gcgccggacg
     1681 cggtcgaggt ccagggtccg aagggcaagc tccggcaagc cgttcccccg ggcatcctgt
     1741 ttgccgagga cgccggagcg ctcgtggcca ggctgtcggt cgaagacgac gagctgcgga
     1801 agttccaggg gctggcgcgc agcctcgtgg ccaacgcggt ggctggcgtg accgaagggt
     1861 tcaagaagga gctcgacatc gtcggcgtcg gctatcgcgc ggagctgaag ggcaagcagg
     1921 tgcacttctc gctcggatac tcgcacccgg tgatcttcga catcccggct ggcatcgaca
     1981 tcgctgtgga caagcagacg cacatcgtcg tgacgggggc cgaccgtcag ctcgtgggcc
     2041 aggtcgctgc caatatccgg cggatgcgca agccggatcc ctacaagcag aagggcgtgc
     2101 gatacacggg cgagcagttg aagaagaagg tcggcaagac gggggccaag tagaggcggg
     2161 tcttcagacc cgcctggtga acgagcgatg agaatcaaga cgaaagaaga tcgccgcgac
     2221 cgcatcaagt accgcatccg gaggcgggtg cagggatccc cggagcgtcc gaggctgacg
     2281 gtcttccgga gcgtctcgca catgtacgtg caggtggttg acgacaccac ggggcgcacc
     2341 atcgcatccg cctcgacggt cgagccggcc gtcaagggcg ggctccccaa gaaggctggc
     2401 ggcggcaaca tcgcgggagc caaggcgatc ggcaagacga tcgccgagcg gctcctcggc
     2461 caggggctga agcgggtcgt gttcgatcga aacggctttc tctatcatgg gcgcgtgaag
     2521 gctgtggccg aggccgcgcg cgaagcaggt ctggagttct aacgtggctc tgatgaaccg
     2581 tgagcgcgag aagatcgacg catcgcagct cgagatcaag gacaccgtgg tctcgatcaa
     2641 ccgcgtgacg aaggtggtca agggcggcaa gaacctgagc ttcagcgcgc tggtcgtcgt
     2701 cggcgacgcc cacggcgtcg tcggcttcgg cgtcggcaag gcgaaggaag tgccctcggc
     2761 gatcaagaag gggatcgagg ccgcgaagaa gaacctgatc cgcgttccgc tcaaggggac
     2821 gacgatcccg caccaggtgg tcggccgctt cggctccggc agcgtgctgc tcaagccggc
     2881 gccggaaggg accgggctga tcgcgggcgg cgccgtgcga gccgtcgtcg agagcgccgg
     2941 tatccagaac gtgctcacca agtcgctcgg gtcggcgaat ccgcacaacg tcgtgcgtgc
     3001 cacctttgcc ggcctcatgg gactgaagga tccgggacag gtggccagat tccgcggcaa
     3061 ggaattagcg gagctggtgg gggcgtagat ggcgaagaaa gcagcgaaga agacgagtgc
     3121 caagcggctc aagatcacgc tcgtcaagag caccatcggc ttcaaccgca cgcaggacga
     3181 cacggtcacc ggtctgggcc tgcggcggct tcggcacacc gtggaactgc cggatacgcc
     3241 cgagacgcgc ggcatgattc acaaggttcg gcatctggtg gtggttcagg agtagcgcga
     3301 tggatttgag cgatctcaag cccgccaagg gttcgaagaa gaaccgcaag cgcgtcggcc
     3361 gcggtcacgg gacgggccag ggcacgcagg ccgggcgggg ccacaaggga gcgcagtcgc
     3421 gctcgggctt caagttcaag cgcggcttcg agggcggcca gatgccgctg caccggcgca
     3481 ttccgaagcg cggattccac aacccgttcc gcgtcgagta tgtcgtcgtg aacctcgaca
     3541 ccctggccga gcgcttcgag tcgggaacgg tggtcacgcc ggaactgatg cgcgcgaagg
     3601 ggctcgtcag cagcgctcgc gggctgatca aggtattggc ccgcggagag gtctcgaaga
     3661 agctgaccgt gcgcgcgcac aagttcagcg gcaaggcggc ggagaagatt gcggcggccg
     3721 gaggcgcggc cgaggtgatc gcgacggtga ccgcggcagt gggcaaagat ggagaacgcg
     3781 cttaagaacc tgtttgcggt caccgatctt cgcaaccgcg tgctcttcac gctggcgctg
     3841 ctcggtgtgt accggatcgg cgctcgcgtg ccgacgcctg gcgtgaacca ggaggcgctg
     3901 cgcctgtttg cagaccagct gcagggctcg atgttcggcc tcgccaacat gttcacgggc
     3961 ggcagcctgg agcgcgtgac catcttcgcg ctcggcgtga tgccgtacat cagcgcgtcg
     4021 atcatcattc agctgctgac agtcgtgtgg ccgtatctcg agcgcatctc gaaggaaggc
     4081 gagctcgggc gccgcaagat cacgcagtac acgcgctacc tgaccgtcgt gctggccgtg
     4141 gtccagtcct tcggctacgc cgtctggctg gaaagcacgt cagacgcgcc cggcggcctg
     4201 ccgctcgtgt tcgacccggg ctggggcttc cgcctcatgt gcgtcctcac gctgacgacc
     4261 gggacgatgt tcgtgatgtg gctgggcgag cagatcaccg agcgcggcat cggcaacggc
     4321 atgtccctgc tgattttcgc aggcatcgtc gtcggtcttc cgacggcgct gctggccacg
     4381 ctggaccagc tccagacgca gcagatcacc ctcttgcgcg tcgccttcct ggtcgtgatg
     4441 gcggttgtcg tgatcggcgt gatcgtgttc gtcgaacgcg cgcaccgccg catcactgtc
     4501 cagtacgcca aacgtgtcgt cggccggcgg cagtacggcg ggtcgagcac ccacatcccg
     4561 ctgaaggtca acacgggcgg cgtcatcccc gtgatcttcg cctcgtcgat cctgtcgttc
     4621 ttcacgatgg ccgcgccgct gttcgaggtc ggaacctggg cgcgacgcgc gctcgatcaa
     4681 gtcgcctacg gcatgcccct gtacaacctg ctgtacgtcg tgggcatcat cttcttcgcc
     4741 tacttctaca cgtcgatcat cttcaacccc gacgacgtgg cggagaacat gcgcaagtac
     4801 ggtggcttcg tgcccggcat ccggcccggg aagcggaccg ccgagtacat cgacaccatc
     4861 ctcgcgagga tcacgctggt cggggcgctc tacctcgcgc tcgtcgcgct gctccccgag
     4921 ctgctcatca gcgggttccg cgtggcgccg atcccgtaca tcggagagac gctggacgcg
     4981 atcctgccgc cctggttcac gcagggcctt ggcgtcacgt tcttcttcgg cggcacgtcg
     5041 ctgctgattg tggtcggcgt cgcgatggac accgtgcagc aggtggaatc gcagctcatc
     5101 atgaggcatt acgacggctt catgaagaag acgcgcatcc gcggacggcg gggttagcgt
     5161 ggcacggtct ggaagatggg cttgaatgtg gttgtgatgg gaccgcctgg ggctggcaag
     5221 ggcacgcagg cggggcggtt tgcccgggat cgcggtctgc cgaggatcgc gacgggcgac
     5281 atcctgcgag agggagtcaa ggagaagaat ccgatcgcgc tcgaggccag ggcccggatg
     5341 gatcggggcg agctcgtcga cgatgcgacg atgatcaaga tcgtggccga tcggctgttg
     5401 aggccagatg tcgacgccgg gttcgtgctg gacggctttc cgcgcacggt cgcccaggcc
     5461 cgcgcgctcg acgagctgat tggacagcgt ggacgcgggc ccctcatcgt cgtggatctg
     5521 gccgtgccgg atgaagaaat tgtgcggcgc ctggcgggcc ggcggatctg ttccgagtgt
     5581 ggaacgaacg aggatccgca gtacccgggc gatcgctgca ggaactgcgg aggccgcctc
     5641 gtccagcggg gcgatgacga ccggaaggtc gtgcaggagc gtttgagggt gtatcagcag
     5701 tccacgaggc cgctgctcga ctactaccgg gatcggccga cgtttcgcgc ggtcaacggc
     5761 gcgcagcccc ccgagctggt ggcccgcgaa ctggacgcga tgattgacgc cgcggcggcg
     5821 gcgcggcggt agggagaagg gcgtgatcgt ctgccggtcg aagagcgaga tcgacaagct
     5881 ccggcgggtc aatcagctcg tcgcgcggat tctcgcggag ctgaggcgga tggtgacgcc
     5941 cggcgtgacc accgaggcga tcgacctctt cgcggagcgg caggtgcggg atgccggcgc
     6001 ggagccggcg ttcaaggggt accacgacta tccggcgacg gtgtgcgcgt cgatcaacga
     6061 acaggtcgtg cacggcattc cgtcgtctcg ggcgctcgtg agcggtgaca tcgtgtcgat
     6121 cgacatgggc gccaggctgg atgggttctt tggtgactgc gcagtgacgg ttccggtggg
     6181 gcacgtgacg ccggacgcgg cccggctgct ccaggtcacc gaggaggcgc tgttcagagg
     6241 aatcgactgc gttcgacccg gcgcccgtgt ctcggacatc ggcgcggcgg tccagcagca
     6301 cgtggaggcg cagggctact cggtggttcg ggagttcgtc ggtcacggca tcggaaccga
     6361 gctgcacgag gagccgcagg tggccaacta cggtccggct gggcgcggtc cgcggctgag
     6421 cgagggcatg gtgctggcga ttgagccgat ggtgaatgcc ggcctcccgg gggtcaagat
     6481 tttgtcggat cgatggacgg cagtgaccag cgacaggagc ctgtcggcgc acttcgagca
     6541 caccgtcgtg gtgacgcgcg acggcgtgga gattctgacg ctgttgcccg aggacgaggc
     6601 acgcgccagg cagctgctgg ctgcggcgcg gagtccgaac gggcagggac gcgacgagtc
     6661 ggagggttat ccggcccgta cgtgagtacg gcagccggcc gaggagatga gccgggagga
     6721 tgtgcgccgc gtcgaggctg tcgtgacggc tcagctgccg agcggcctgt atcaggtgga
     6781 ggtcggcggc gagcagcgct tgacggctca ccccgcgagc ggcacggagc gcaatttcgt
     6841 caggctgctc gtcggcgatc gagtggtcgt ggaagtgacg gcgcgtgacg ggacacgcgg
     6901 ccgaatcgtc cggaaggcgt aggtatgaag gtcagagcat cggtgaagcg catttgcgac
     6961 aagtgcaagg tggtccgccg ccgaggggtc gtgcgggtga tttgcaccaa cgcgaagcac
     7021 aagcagaggc agggataacg agatggcacg catcgcaggc atcgatcttc cgcgcaccaa
     7081 gcgggtcgaa atcggactga cctacatcta cggcatcggc cggaagcgct ccggcgacat
     7141 cctgggcgcg gcgggcgtga gccccgacgt gcgggtcaag gacctgtcgg aggatgacgt
     7201 ccggaagatc agccgcgtca tcgaagaagt gggcggcgtc gagggcgatc tccgcaagga
     7261 ggtctccatg aacatcaagc ggttgatgga gatcggctgt taccgcgggc tgcgtcaccg
     7321 ccggaacctg ccggcgcgcg gccagcggac gcgcaccaac gcccgcacgc ggaaggggcc
     7381 gcgcaagggc gcggtcgcca agaagaagac gacagtctga taggacgata tggccaagac
     7441 cgaagcggca ccagacaaga aagccgaggg cgtcaagaag gcgccacgca agacgttcaa
     7501 gaagcgcggg gagaagcgcg tcgtccacca cggactggtg cacatccacg cctcgttcaa
     7561 caacacgcag attacgatca ccgacaccga ggggaacgtg atcgcctggt cgagcgccgg
     7621 cggcatcggc ttcaaggggt cgcggaaggg cacgccgttc gcggccacgc aggccgccat
     7681 gagcgccggg aacacggcga agacctacgg cctcaggtcg gtggacgtgc gtgtcaaggg
     7741 cccggggtct ggacgcgagt cggcgatccg ggcgctccag accgtcggta tcgaggtgaa
     7801 gtcgatccgc gacgtgacgc caatcccgca caacgggtgc cggcccacga agcgacgaag
     7861 ggtctgacgt gaaacgctgc attgactgag taggagtacg aatggctcgt tacatcggac
     7921 ccgtgtgccg tctgtgccgc cgggagggca tgaagctctt tctcaagggc gagcgctgct
     7981 acaccgagaa gtgtgcgatc gagaagcgca acttcgcgcc cggccagcac ggcaagaccc
     8041 ggaagtcgaa gctggctggc tacggcgttc agctgcgcga gaagcagaag gtcaagcgca
     8101 tctacggcgt gctcgaggac cagttccgcc gctacttcga gcaggcagaa cggacgcgcg
     8161 gcattaccgg cgagacgctg ctgcagctgc tcgagcggcg cctggacaac gtcgcctacc
     8221 ggctgggctt cgcgacgtcg cggccgcagg cgcgccagct cgtgcgccac ggccatttca
     8281 ccgtcaacgg caagaaggtg gacatcccct cgttctcggt gaagcctggc gacgtcgtcg
     8341 ccgtgcggca gagcagccgg aagtcggcgg ccatcctgca cgcgctcgaa gaggtcaagg
     8401 gccgcggcgt acccgagtgg ctgcagttcg acgccgaagg gttgtcggcc aagattggct
     8461 ccgtgccgac gcgcgagcag atcaacctgc ccgtgcagga gcagctgatc gtcgagctct
     8521 attcgaagta gcgcgtgggc gcttagcccg cgcgtcgttc gcatccgccg gcccgcagcc
     8581 caacagacct gcggcgggag gccggcggac aacaactgtg caggtctgga ggccggggct
     8641 gccggcccgt tgcgaaagga acgtccatgc tctggaaagg ttttcagcgt cccaagcgtc
     8701 tcgagttcga acgggagacc ctcaccgaca ggttcgggcg gttctacgcc cagccattcg
     8761 agcgcgggtt cggtaccacc atcggcaatg cgcttcgccg cgtgctgctg tcctcgattg
     8821 aaggcgccgc catcacggct gtcaagatcg atggcgtgct tcacgagttt tcgccgattc
     8881 cgggcgtggt cgaggacgcc acggacatca tcctgaatct caagcagatt ccgctcaaga
     8941 tgcacgtgga caccaccaag accctctacc tgcgcgtgga caagccgggc gaggtgaagg
     9001 cgcgcgacat cgagacggac gccgacat
//
LOCUS       DBNGSH010000744         1766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3647343, whole genome shotgun sequence.
ACCESSION   DBNGSH010000744 DBNGSH010000000
VERSION     DBNGSH010000744.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3647343"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="AC16CY_17075"
     CDS             <1..227
                     /locus_tag="AC16CY_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartyl protease"
                     /protein_id="HIR3355566.1"
                     /translation="KAHSKQVFTLADGSQITRRRGDALFAMNGHRGAAPVIFAERGDS
                     TLLGVVTLEALGVILDPIKRELRPLPMILA"
     gene            complement(256..1077)
                     /locus_tag="AC16CY_17080"
     CDS             complement(256..1077)
                     /locus_tag="AC16CY_17080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3355567.1"
                     /translation="MRVAATAALVLVGAGRLLAQAPTIEPVRTDTLAHPDDASKRIEI
                     YWTKPAGEGPWPVLLMVHGHQEPERPGAKAYVDSGSFERYASRGLLVAAVSQPGYGKS
                     DGPPDFCGPRSQRAVIAAIDHFRKQPFVDAKRMALYGYSRGAVVASMVSSQVPDLAAV
                     VLGGGIYDLKATYRRMARGIQRNIDQEAGKSDEAYRARSALFHVDRIKSPTLVLHGER
                     DDRASAESAKAFGAALEKTGTPVRVVIFPGVGHGIPPAEQTREWAPFLRQYLGIK"
     gene            complement(1087..>1766)
                     /locus_tag="AC16CY_17085"
     CDS             complement(1087..>1766)
                     /locus_tag="AC16CY_17085"
                     /inference="COORDINATES: protein motif:HMM:NF013360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HIR3355568.1"
                     /translation="LTSAAPADAQRTSVGREFPVYDNGGRPDLTVDPKRFVSQMEIVD
                     RFFDPIDDACAFAEEVIGGAGYRRLLRFDTVVLNAGDADLVVGDRSDPGNPYAPAFEF
                     SPCHGHYHIKDFSEYSLLRPNGTVAAAGHKQGFCFEDSLKYGNNRSSGFDCSFQGITS
                     GWGDWYYKQLPGQWIDITGVPEGDYIVRATINMAGTFDEGTDLYPDVIEVRIHVPDPR
                     KKVTIVE"
BASE COUNT          291 a          606 c          562 g          307 t
ORIGIN      
        1 tgaaggccca ctcgaaacag gtcttcacac tggcggacgg gtcgcagatc acgcggcgac
       61 ggggagatgc gctgttcgcg atgaatggtc atcgtggcgc cgcgcccgtc atcttcgcgg
      121 agcgtggtga cagcactctg cttggcgtgg tcacgctcga ggcgctgggg gtcatcctcg
      181 atccaatcaa gcgcgaactt cgcccgctgc cgatgatcct ggcctgaacg gctcccggtc
      241 gaagagccga aagccctatt tgatccccag gtactggcga aggaacggcg cccactcgcg
      301 cgtctgctcc gccggcggga tgccgtgacc gacgcccggg aagatgacca cgcgaacggg
      361 cgtcccggtc ttctccagcg ccgccccgaa ggccttcgcc gattcggcgc tcgcgcgatc
      421 gtcccgctcg ccgtgcagca cgagcgtcgg cgatttgatt cggtccacat ggaagagcgc
      481 cgagcgcgcg cggtacgcct cgtcggactt gccggcctcc tgatcgatgt tccgctggat
      541 cccccgggcc atcctcctgt aggtggcctt cagatcgtag atgccgccgc ccagcacgac
      601 ggccgcgagg tccggcacct gcgaggacac catcgacgcg acgacagcgc cgcggctgta
      661 cccgtacaag gccatccgct tcgcatcgac gaacggctgc ttccggaagt gatcgatcgc
      721 ggcgatgacc gcgcgctgcg agcgcggccc gcagaaatca ggcgggccat ccgacttgcc
      781 ataccccggt tgtgacaccg ccgcaaccag gaggccgcgg ctggcgtatc gctcgaaact
      841 gccactgtct acgtacgcct tcgcgcccgg ccgttcgggc tcctggtgcc cgtgaaccat
      901 gaggaggacc ggccacggcc cttcccctgc cggtttcgtc cagtaaatct cgatgcgctt
      961 cgacgcgtcg tccggatggg ccagtgtgtc ggtccgaaca ggctcgatcg tcggtgcctg
     1021 ggcaagaagc cgacccgccc ctacgagaac gagcgccgcc gttgccgcaa cgcgcatcat
     1081 cgcgtcttac tcgacgatcg taaccttctt gcgcggatcc ggcacgtgga tccgcacttc
     1141 gatcacgtcg ggatagaggt cggttccttc atcgaaggtg ccagccatgt tgatcgtggc
     1201 ccggacgatg tagtcgcctt cgggcacccc ggtgatgtcg atccactgac cagggagctg
     1261 cttgtagtac caatcgcccc acccgctcgt gatcccctgg aagctgcagt cgaacccgct
     1321 gctccggttg ttcccgtact tgagagagtc ctcgaagcag aagccctgct tgtgccccgc
     1381 ggcggcgact gtcccgttgg gacgcagcag cgagtactcg gagaagtcct tgatgtgata
     1441 gtggccgtgg cacggtgaga actcgaaggc cggcgcgtac ggattgccgg gatcggacct
     1501 gtctccgaca acgagatcgg cgtcgccggc gttgaggacg acggtgtcga atctcaggag
     1561 gcgccgatag cctgctccgc cgatcacctc ctcggcgaac gcgcacgcat cgtcgatcgg
     1621 gtcgaagaac cgatcgacga tctccatctg cgacacgaac cgcttcgggt ccaccgtcag
     1681 gtccggccgt ccgccgttgt cgtacacggg aaactcgcgg cccacggacg tccgctgcgc
     1741 gtcggccggc gccgcgctcg tcagac
//
LOCUS       DBNGSH010000745         8884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4667454, whole genome shotgun sequence.
ACCESSION   DBNGSH010000745 DBNGSH010000000
VERSION     DBNGSH010000745.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4667454"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="AC16CY_17090"
     CDS             <1..1314
                     /locus_tag="AC16CY_17090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3355569.1"
                     /translation="HVALRHGTNQASKASAAQIPLAILGGLLSTNSTRTALTQLGANF
                     AVNSVLLKYSRTAESQADIMATQILHDSGYDPRAMAQFFQKIEAEEHGSGPVEFFSNH
                     PSPDNRIERVNQEVKALGGARRDATTNSREFDEIQRYVRSLAGPPVKGQLQAGEDGRQ
                     PVASELRILSASYGAGDRFTDVRQRLQSRVQNERLDLKVNTSSMGSDPSRGQAKTLRL
                     RYDWAGRSYDVTVADNQRLLIPTDQQVSAAADGTGTITDWPSSRFVSVENSLLRINHP
                     DDWQTHGQGDAMTITPRGGLIDDGQGSQAMAYGVIVNIFEPRGDRVGQQLQGPGAGQG
                     GGQDSAMRLVEQSTDQLVQELRLSNRNMRVIRYREAIRVDGDRALSTYLSNDSPLGGR
                     ETVWLVTVERPDDALLFIVFTAPERDVQRYEGTFHQMLRSVRIKR"
     gene            complement(1317..1607)
                     /locus_tag="AC16CY_17095"
     CDS             complement(1317..1607)
                     /locus_tag="AC16CY_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355570.1"
                     /translation="MTITLPGLIVLIVIAAVCGAVGKALAGGGRGGLIVSTALGFIGA
                     ILGPWIARRLELSEPLVLQVGGQPFPIVWSIIGAALFVAFLHLMSGRRWARA"
     gene            complement(1708..2379)
                     /locus_tag="AC16CY_17100"
     CDS             complement(1708..2379)
                     /locus_tag="AC16CY_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3355571.1"
                     /translation="MKRARLVAQAISKEFDAQRFLESAGAARQIVTHRKGSVIFRQGD
                     ASSDVIYIRKGGIKLSVLSPGGKEAVIALLGPGDFFGEGVLAGQSVRIGTATATMSTT
                     ILSIKRKTMLRLLHEEHAFSDRFIAYMLERNLRIESDLVDQLFNSSEKRLARALLLLA
                     RYGERASPDRRIPTISQETLAEMVGTTRSRVNFFMNKFRKLGLIEYNGGLKINNSLLS
                     VVLND"
     gene            complement(2449..5112)
                     /locus_tag="AC16CY_17105"
     CDS             complement(2449..5112)
                     /locus_tag="AC16CY_17105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3355572.1"
                     /translation="MRKTPMWRKYLRFWGADPPADVDTEISFHFEELVKHYVARGMTA
                     DRARSEASRRFGSIARVRAECVTIDEGSVRAARRRDAADALWQDVLDACRGLTRNPGF
                     TVGAALILAVGIGFNTTIYSFNKALFFPSLPLGDAPHLVRISAQNMARGAFAAPLSEG
                     ESADVTAGNRSFLDVAAFVIRPATLTGGTQVERIAVMRATTNLFSLLQVQPALGRDFR
                     SDEAAGDGSPVAILSHRAWENRFAGDRMVVGRDIHLDGRPHTVIGVMPDGFWFESRHI
                     EAWLPHPRLRADGARDARTLTTVARLDPHISPQAAQADMQALGERLAQDHPGTNSGWN
                     ILVTGLLRLGPGEETFFALVTTLLGLLLAAACAHVANLLLARGMERRGEIAIRAALGA
                     GRGRILRQLFAESVALAVVGGVCSLLVTFPILRQIRAVLGSRTPLLENLSLDGMALGI
                     TAGLIVVASVLFGLAPALRLSTVTAGDAMKQPPGGPITGRRRRPLASLMIGLETAVAT
                     LTVIVSVLFVRASSNVLAIPLGFDTKGVLTFRIEPPDYKYPLQEAAARAMTEIHARLR
                     ELPSIGAVGAAVRLPLNFGPGLPTEAVTIDGRQDRPQEQSPWAVTAVVTPGYFEALGI
                     PVLQGRSFDDGDAASSAPVALVSRSVQQAYWPDQAAVGRRLRLGGADATGPWLTVIGV
                     VDDVRPFDPSSPQVRQLYLPLAQNPVRALTYFVATGADPASRFQDVRLAVRETDPELP
                     VLDLGTLTSAIDSALSGPQLAARSISANAVIALLLALTGVYSVVTFASSRRRREIAIR
                     VALGGRRSAVVTMLLRQALAPTFAGIVIGLALAAIATRAIAVLLYGVNPLDPLTYALT
                     AASLTAAAAAASCVPAIRATRVNAALALRAE"
     gene            complement(5127..5471)
                     /locus_tag="AC16CY_17110"
     CDS             complement(5127..5471)
                     /locus_tag="AC16CY_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3355573.1"
                     /translation="MGDAPLSRNPDMLRGTVDLLILKALTHGRQHGFGIARWLEDVTG
                     DVLRLEEGSLYPALHRMERRGWLESEWGVSDNNRQVKYYRLTRRGREYLQRETSGWTE
                     FATAVFKVLQTT"
     gene            complement(5537..6019)
                     /locus_tag="AC16CY_17115"
     CDS             complement(5537..6019)
                     /locus_tag="AC16CY_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355574.1"
                     /translation="MSHRVVRAGVLIALAVASAIAVSAQVGGKPVIGGNPTPGTTQPD
                     PPNLSDRITLSGCLQRAPKGANATEADANTPSDSRFVLSGAARVEKVPAGTGQSDLAK
                     KAGGGTYRLEGLDSIFSPFVGTKVEISGEVKARAAAEAGGATTSTLIAEFVRRIAARC
                     "
     gene            complement(6016..7404)
                     /locus_tag="AC16CY_17120"
     CDS             complement(6016..7404)
                     /locus_tag="AC16CY_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide isomerase"
                     /protein_id="HIR3355575.1"
                     /translation="MVRYGLAACLIGVLAVAAGDTGTTAAQAPVTFNKDVLPVLQKNC
                     QSCHRPGQMAPMSLVTYRDARPWARAMKTKVESRQMPPWFADSAYGPYANDRSLSSAD
                     IETIAKWADAGAPEGNPNDAPPAVNWPGDGWQITPDIVVRGPEFRVPARTPNDVVEWI
                     TYLIPSGFTEDTWITSLEIKPSAPEVTHHICFTFQQHRPNAKYYVPVWSESPRDDEGA
                     AINGSNPPRQAVGGVSAQTGQPGTAVGGGFNCWVPGRAADDYRAFGAGKLVPAGVDIS
                     IQVHYTPYGKEIVDRPLIGFTVADKAPAKRWVSFGIVGGGPDFAIPPNEPNYKSPPFD
                     LTFAADVELVEFMPHMHVRGKDMTYHLVYPDGRDQIVLSVPKYDFNWQLLYQPAKPIR
                     VPKGTRMYVDAHYDNSRANRFNPNPNRTVHLGRMTWEEMMAPFFGILVDSNVNPNDVL
                     RLGRFAVQGDGA"
     gene            7766..7990
                     /locus_tag="AC16CY_17125"
     CDS             7766..7990
                     /locus_tag="AC16CY_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR3355576.1"
                     /translation="MPRSLAVIHSDPEILGGTPVFVGTRVPFRNLIDYLERNHSLDEF
                     LDAFPTVSREQAVAALEAAHEAVTARARSA"
     gene            7974..8297
                     /locus_tag="AC16CY_17130"
     CDS             7974..8297
                     /locus_tag="AC16CY_17130"
                     /inference="COORDINATES: protein motif:HMM:NF037832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR3355577.1"
                     /translation="MRVLLDEQLPRQLAPLLVGHEVRTVQQQSWAGLKNGQLLEAAEA
                     AGFTVLVTGDRNLQFQQNLSNRKLGVVVLCSASNAIEDLTPLVQAAVSAIEVVQAGEV
                     LRVET"
     gene            complement(8683..>8884)
                     /locus_tag="AC16CY_17135"
     CDS             complement(8683..>8884)
                     /locus_tag="AC16CY_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355578.1"
                     /translation="TEPAVLRVLHVDPAVPLTPDAMYVVMKREPELVEFLQCDLFRAS
                     PLFKIVGPDYLKAWMAIHPCGG"
BASE COUNT         1555 a         2946 c         2921 g         1462 t
ORIGIN      
        1 cacgtcgcgc tgcgccacgg caccaaccag gccagcaagg cctccgcggc gcagattccg
       61 ctggcgattc tgggcggcct gctcagcacc aactccacca ggacggcgct cacgcagctc
      121 ggcgcgaact tcgccgtcaa ttccgtcctg ctgaagtact cgcgcacggc cgaaagccag
      181 gccgacatca tggccaccca gatcctccac gattcggggt acgacccgcg tgccatggcg
      241 cagttcttcc agaagatcga ggccgaggag cacggcagcg gcccggtcga gttcttctcc
      301 aaccacccga gcccggacaa ccgcatcgaa cgcgtgaacc aggaagtgaa ggcgctcggc
      361 ggcgcgcggc gcgacgccac gaccaattcc agagaattcg acgagatcca gcgctacgtc
      421 cggtcgctgg cggggccgcc cgtcaagggc cagttgcagg ccggcgagga tggccggcaa
      481 ccggtcgctt cggaattgcg catcctgtcg gccagctatg gcgccggcga tcggttcacc
      541 gacgtgcgtc aacgactgca gtctcgcgta cagaacgaac ggttggactt gaaggtgaac
      601 acgtcgtcga tgggcagcga ccccagccgg ggccaggcca agacgcttcg tctccggtat
      661 gactgggccg gccggagcta cgatgtgacg gtggccgaca atcagcggct tctcattccc
      721 accgaccaac aagtgagcgc cgccgccgac gggaccggga caataaccga ctggccgtcg
      781 tcacgcttcg tgagcgtgga gaactcgctg ctgcgcatca accaccccga cgactggcag
      841 acccacggac agggcgacgc gatgaccatc acgcctcgcg gcggcctgat cgacgacggg
      901 caaggcagcc aggcgatggc ctatggcgtc atcgtgaaca tcttcgagcc acgcggggac
      961 cgcgtcggcc agcagttgca aggtcctggc gccgggcaag gtggcgggca ggattcggcg
     1021 atgcgtctcg tcgaacagtc cactgaccaa ctcgtccagg agctgcgcct gtcgaaccgg
     1081 aacatgcggg tgattcggta tcgcgaggcc atccgcgtgg atggcgaccg tgcgctatcc
     1141 acgtacctct cgaatgactc tcccctcggc ggtcgtgaga ccgtctggct ggtcaccgtc
     1201 gagcgccccg atgatgcctt gctgttcatc gtgttcaccg ctccggaacg cgacgtccag
     1261 cgctacgaag gcacgttcca ccagatgctg cgatcggtcc ggatcaaacg gtaggttcag
     1321 gcccgcgccc agcgccgccc ggacatgagg tgaaggaacg cgacgaacag ggcggcgccg
     1381 atgatggacc agacgatcgg aaacggctgg ccacccacct ggagcacgag cggctccgac
     1441 agctcgagcc gcctcgcaat ccacggcccg aggatggcgc cgatgaaacc aagggcggta
     1501 gacacgatca gcccgccccg ccctccgcct gcgagcgcct tgccgacggc gccgcagacg
     1561 gctgcgatca cgatcagcac gatgagtccc ggcagtgtga tggtcatcgc tgcatggtag
     1621 gggcgattcg tgccgccgtc tgtgcctgat tgcacagcgc cctcagcccg cctcgctggc
     1681 cggcggcttg aacgccgcgc tcgagggtca gtcgttcagg acgacgctga gcagggaatt
     1741 gttgatcttg aggccgccgt tgtactcgat cagcccgagc ttcctgaact tgttcatgaa
     1801 gaagttcacg cgcgaccgtg tcgtgccgac catctccgcg agcgtctcct gtgagatagt
     1861 ggggatcctg cggtccggac tggcccgctc gccgtagcgc gcgaggagca gaagcgcccg
     1921 ggccagccgc ttttcgctgg agttgaagag ctggtcgacg aggtccgact cgatgcggag
     1981 gttcctctcc agcatgtacg cgatgaagcg gtccgagaag gcatgctcct cgtggagcag
     2041 cctgagcatc gtcttcctct tgatggatag gatcgtcgtg gacatggtcg cggtcgccgt
     2101 cccgatccga accgactgac cggccaggac tccttccccg aaaaagtccc cggggcccaa
     2161 taacgcgatc actgcctctt ttccgccggg cgacagcacc gagagcttga tcccgccctt
     2221 ccgaatgtag atgacgtcgc tggaggcgtc gccctgcctg aatatgacgc ttccctttcg
     2281 atgcgtcacg atctgcctag ccgcgcccgc cgattcgaga aaccgctgcg cgtcgaactc
     2341 cttcgagatg gcctgcgcga caagccgagc tcgtttcatc gtgcggggac tccatcttct
     2401 ccccggtgca aggctagcac aaacgtggac gactcgcgcc cgcgggggct attccgccct
     2461 gagtgcgaga gccgcgttca cgcgggtggc gcggatcgcg gggacgcagc tcgcggcagc
     2521 agcagcggcc gtgagggagg ccgccgtcag cgcgtatgtc agcggatcga gagggttgac
     2581 gccgtagagc agcaccgcga tcgcacgggt cgcgatcgcg gccagcgcga gcccgatgac
     2641 gatgccggca aacgtcggcg cgagcgcctg ccggaggagc atcgtcacga cggccgagcg
     2701 ccgtccgccg agcgcaacgc gaatggcgat ttcgcggcgc cggcgggagg aggcgaacgt
     2761 gacgacggag tacacaccgg tgagggcgag caggagcgcg atgacggcgt tcgccgatat
     2821 cgatcgcgcg gccaattgcg gtcccgacag cgcgctgtcg atcgcgctcg tcagcgtgcc
     2881 gagatccagt accgggagct cggggtccgt ctctcgtacc gccagccgaa cgtcctggaa
     2941 ccggctcgcg ggatcggcgc ccgtcgcgac gaagtaggtg agggcccgca cggggttctg
     3001 tgcgagtggt agatagagct gccgcacctg cggggaagac ggatcgaagg gccgcacgtc
     3061 gtcgaccacg ccaatcaccg tgagccacgg cccggtcgca tcggcgccgc cgagcctcaa
     3121 ccggcggccc accgcggcct ggtccggcca gtacgcttgc tgcaccgatc ggctgacgag
     3181 agcaacgggt gcggacgagg cagcatcacc atcgtcgaag gaccgcccct gcagtacggg
     3241 aatgcccagt gcctcgaagt acccgggcgt cacgacggcg gtgacggccc acgggctctg
     3301 ctcctggggc ctgtcctgcc gtccgtcgat cgtcacggcc tcggtgggaa ggccgggccc
     3361 gaagttcagc ggcagccgga ccgccgcgcc cactgcgccg atggacggca gttctcgaag
     3421 gcgcgcgtga atctccgtca tcgcgcgcgc ggccgcctcc tgcagaggat atttgtagtc
     3481 cggcggttcg attcgaaagg tcagcacgcc tttggtgtcg aacccaagcg gaatcgccag
     3541 cacgttgctg gacgcccgca cgaagaggac actgacgatc acggtgagcg ttgcgacggc
     3601 ggtttcgagc ccaatcatca ggctggccag cggccgccgt cgccggccgg tgatcggccc
     3661 gccgggcggc tgcttcatcg cgtcgccggc ggtgacggtc gagagtcgaa gggcgggtgc
     3721 gaggccgaac agcacgctgg cgacgacgat caatccggcg gtgatgccca gcgccatgcc
     3781 gtccagtgaa aggttctcga gaaggggcgt tcgcgatccg agaacggcgc gaatctgcct
     3841 gaggatcgga aaggtgacca gcaggctgca cacaccgccg acgaccgcca gcgccacgct
     3901 ctcggcgaac aactgacgga ggatgcggcc gcggccggcg ccgagcgccg cccgtattgc
     3961 gatctcgcct cggcgttcca tgccgcgagc gagcagcaga ttggccacgt gtgcgcacgc
     4021 ggcagcgagc agcaacccca ggagcgtcgt gactagggca aagaaggtct cctcaccggg
     4081 gcctagccgg agaaggccgg tgacgagaat gttccatcca ctgttggtac ccgggtggtc
     4141 ctgcgccagc ctctctccga gcgcctgcat atccgcctgg gccgcctgcg ggctgatgtg
     4201 cggatcgagc cgtgcgacgg tcgtgagcgt gcgggcgtcg cgggcgccgt cggcgcgcag
     4261 tctcgggtgc gggagccacg cctcgatgtg gcgcgattcg aaccagaaac cgtcgggcat
     4321 gacgccgata accgtatgag gccgtccgtc gaggtgaatg tcgcgtccca cgaccattcg
     4381 atcgccggca aagcggttct cccacgcccg atggctcagg atcgcgaccg gacttccgtc
     4441 ccctgcagcc tcgtcagaac ggaagtcacg gcccaacgca ggctggacct gcagcaggga
     4501 gaagagattc gtcgtcgccc gcatgacggc gatccgctcg acctgcgtgc ccccggtgag
     4561 cgtcgctggt cgaatgacga aggccgccac gtccagaaac gatcgattcc cggcagtgac
     4621 gtcggcagac tctccttccg acagcggggc ggcgaaggcg ccgcgagcca tgttttgtgc
     4681 cgagatcctg acgaggtgcg gcgcgtcacc gagcgggagg gaagggaaga acagggcctt
     4741 gttgaagctg tagatggtag tgttgaagcc gatgcccacg gcgaggatga gcgccgctcc
     4801 aaccgtgaag cccggattcc tcgtcaaccc gcggcaggcg tcgaggacgt cttgccacag
     4861 cgcgtcagcc gcgtcccttc gcctcgccgc gcgcaccgat ccctcgtcaa tcgtcacgca
     4921 ttccgcccgg acgcgcgcga tgcttccgaa acgccgggag gcctcgctcc gtgcccgatc
     4981 ggcggtcatg ccgcgcgcga cgtagtgctt gacgagctcc tcgaagtgaa acgaaatctc
     5041 ggtgtcgacg tcggccggcg gatcggcccc ccagaaccgc agatacttcc gccacatcgg
     5101 cgtcttgcgc atgcgttctg cttccgctac gtcgtctgga ggaccttgaa aaccgccgtg
     5161 gcgaactcgg tccacccgga tgtctcacgc tgcagatact cccggccgcg acgcgtcagg
     5221 cggtagtact tgacctgacg gttgttgtcg gagacgcccc attccgattc cagccagccc
     5281 cgccgctcca ttcgatgcag cgcgggatag agcgaaccct cttccagccg aagcacgtcg
     5341 cccgtgacgt cctcgagcca ccgtgcgatc ccgaagccgt gctgacggcc gtgcgtcagc
     5401 gccttgagga tgagcagatc gaccgtgccc cggagcatgt ccgggttgcg ggaaagcggc
     5461 gcgtctccca tagacaactt atgtgaaagg ggagtctatc agcctttcac atagactgtc
     5521 aatgggagtg ggacggtcag cagcgggcgg cgattctccg gacgaactcc gcgatcagcg
     5581 tcgaagtcgt ggcgccaccg gcctcggcgg cggcccgagc cttgacctcg cccgagatct
     5641 ccaccttcgt gccgacgaac ggggagaaga tgctgtcgag cccctcgagc cgatacgtcc
     5701 ccccgccggc cttcttcgcg agatcggatt ggccggtccc ggccggcacc ttctcgacgc
     5761 gcgccgcgcc cgacagcacg aagcgcgagt cgctgggcgt gttggcgtcg gcctccgtgg
     5821 cgttggcgcc tttcggcgcg cgctgcaggc agccagacag cgtgatgcgg tcgctcaggt
     5881 tcggaggatc gggctgcgtg gtccccggag tggggttgcc gccgatcacc ggctttccgc
     5941 cgacttgtgc cgaaaccgcg atggccgacg cgacggcgag cgcgatcagg acgcccgccc
     6001 gcacgacacg gtggctcatg caccgtctcc ctggacggca aagcggccga gcctgagcac
     6061 gtcgttcgga ttcacgttcg agtcgacgag gatgccgaag aagggcgcca tcatctcttc
     6121 ccacgtcatc ctgcccaggt gcaccgtgcg atttggattc gggttgaacc ggttcgcccg
     6181 cgaattgtcg tagtgcgcgt ccacgtacat cctggtgccc ttgggaacgc gaatcggctt
     6241 tgcgggctga tacaggagct gccagttgaa gtcgtacttc ggaacgctca gcacgatctg
     6301 atcgcggccg tccggataca ccaggtgata cgtcatgtcc ttgccgcgca cgtgcatgtg
     6361 cggcatgaac tcgaccagct cgacgtccgc ggcgaaggtg agatcgaacg gcgggctctt
     6421 gtagttcggc tcgttcggcg gaatcgcgaa gtcggggccg ccgccgacga tgccgaacga
     6481 cacccagcgc ttggccggcg ccttgtcggc gaccgtgaag ccgatgagcg gacggtccac
     6541 gatctctttg ccgtacggcg tgtaatgcac ctgaatagag atgtcgactc ccgcggggac
     6601 cagtttgccc gcgccgaacg cgcgatagtc gtcggccgct cgtcccggca cccagcagtt
     6661 gaagccgccg ccaacagccg tccctggctg gccggtctgc gcggacaccc cgcccaccgc
     6721 ctgccgcggc gggttgctgc cgttgattgc ggcgccctcg tcatcccgcg gcgactccga
     6781 ccagacgggc acgtagtact tcgcgttcgg acgatgctgc tggaaggtaa agcagatgtg
     6841 atgggtgacc tcgggcgcgc tcggcttgat ctcgagcgac gtgatccagg tgtcctcggt
     6901 gaagccgctc gggatcaggt aggtaatcca ctcgacgacg tcattcgggg tgcgcgccgg
     6961 cacacggaac tccggtcccc tgacgacgat gtccggcgtg atctgccagc cgtctccagg
     7021 ccagttgacc gcgggcggag catcgttcgg gttgccctcc ggcgcgccgg cgtccgccca
     7081 cttcgcgatc gtctcgatgt ccgcgctcga gagcgatcgg tcgttggcgt acggcccgta
     7141 cgcggaatcg gcgaaccacg gcggcatctg ccggctttcc accttggtct tcatcgcgcg
     7201 cgcccacggc cgcgcgtcgc ggtaggtcac gagcgacatc ggcgccatct ggccgggccg
     7261 gtggcacgac tggcagtttt tctgcaggac cgggaggacg tccttgttga aggtgacggg
     7321 ggcctgggcg gcggtcgtcc cggtgtcacc ggccgcgacg gccaggactc cgatgagaca
     7381 ggcggcaaga ccgtatcgca ccatggtgtt ttccctatcg agtgcacggt accttaccac
     7441 ggcctcaaca gtcgatctac gaatcgactc tcttcttccc cctcgaccgc gacgcggtga
     7501 tccaagccct gtacgtgggc ccggtcgcgg tgcgggtccg taggtcgctc cgcaaaaccg
     7561 acgtgatcca ggcatttcag cttcgccgcc gaccaacgag gtatgccaat acactcccta
     7621 cggttttgct tcggacctac gcacccgcac cgcggcacat ggcgtcgggc aagacagagc
     7681 agtcacgcag tcggccggat ggagcttggc cagttttagg ctccaacagc atcgccgtcg
     7741 taagatcgct caaagagagc gtcacatgcc gagaagcctg gccgtgattc acagcgaccc
     7801 ggaaatcctg ggtggaacgc ccgttttcgt tgggacgcgc gtacccttca ggaacctgat
     7861 cgactacctc gaacgcaatc acagcctcga cgaattcctg gacgcgtttc caacggtgtc
     7921 gagggagcag gccgtcgctg ccctggaagc agcccacgaa gcggtaacgg cgcgtgcgcg
     7981 ttctgcttga tgagcaactc cctcggcagc ttgcgccact gctggttggg cacgaggtcc
     8041 gaaccgtcca acagcaatca tgggccgggc tgaagaatgg acagctcctg gaagcagctg
     8101 aagccgccgg tttcactgtc ctcgtgaccg gggatcgaaa tcttcagttc cagcagaatc
     8161 tttccaatcg gaagcttgga gtcgtggtcc tctgcagcgc gagcaacgcc atcgaggatc
     8221 tcacgccgct cgtccaggcc gcggtgagtg caatcgaggt cgttcaagcc ggggaggtac
     8281 tccgagttga gacctaagat taacgtgctc aatagccgcg cgcgactgag aggtcaaccc
     8341 gcgtcggctt cctgccgatt gctcgacgtg gatggggaac catctcgaga agagtctgcg
     8401 cggcttcaca tcggaagcct atgctgagtt ttgccgtcgt gcctgcgaac ggcaacctca
     8461 ccagcgccgg catcctgtta ctcggcggta ctcactcact tgagcgtgtg ccccagccgg
     8521 gtccgtggtg tttggttaga gcaatcgtcc cgccacagta gcgcggccct gcgtgccatt
     8581 acgcggtctg tgtcgggggc cgccaggtga acagactctc ggtaaccgcg cgagacgccg
     8641 gcccaccgac gcgttgcagg ctcgcaccga ccagcgtcga gctcacccgc cgcacggatg
     8701 aatcgccatc caggccttga ggtaatccgg tcccacgatc ttgaacagcg gactcgcccg
     8761 aaacagatca cactgcagaa actccacgag ctcgggttcc cgtttcatga cgacgtacat
     8821 cgcatcggga gtgagcggca cggctgggtc gacgtgcaac acccgcagca ccgcgggttc
     8881 ggtg
//
LOCUS       DBNGSH010000746         4931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1950151, whole genome shotgun sequence.
ACCESSION   DBNGSH010000746 DBNGSH010000000
VERSION     DBNGSH010000746.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1950151"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1521)
                     /locus_tag="AC16CY_17140"
     CDS             complement(46..1521)
                     /locus_tag="AC16CY_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HIR3355579.1"
                     /translation="MSARLWLAAPALAAAGILATLLNDPAPVAAAPPSAPAQGSSTLD
                     DQTELSLTVYNSNIALVRDVRNLQVPRGVFDLQFMDIAATVNPATVHFRSLSEPGRVS
                     VLEQNYEYDLLEPDKLLRKYVGRDVTLVRRRQEGGATIEEPVTARLLSYNQAPVWQIG
                     KEIVTGLGADHIRFPEVPGNLYSRPTLIWSLNNDGGQRHRVEASYLATNLTWNADYVL
                     TVGRDDKAADIDGWVTVNNGSGTAFRNARLQLVAGDLNRVQSAVGRMELAERFARQDA
                     AAAREVTQESFGDYHLYTLDRKTTINNNQTKQVSMLGASDFPILKRYIVDGQAFYYRN
                     AQHPGSPLKDVVKVYYQFKNEEKAGLGLPMPSGVVRVYQADSKGGVQFVGEDRIDHTP
                     KDETLNVKIGHAFDVVATRKQIDFEKITSNVYEMEFEIVLRNHKTTAVSVEVNEPIGG
                     TWRMLRSSHQWTKTDAWAAQFSVPVASDGEATLRYRVRVTY"
     gene            1597..2514
                     /locus_tag="AC16CY_17145"
     CDS             1597..2514
                     /locus_tag="AC16CY_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3355580.1"
                     /translation="MLSGNLAGDGVASISDLEQRLTARRYVADRGLAVSLYLALRLRR
                     PLFLEGEAGVGKTSLATAVAEAIDADLIRLQCYEGLDVTHAVYEWDYARQLLELRILE
                     ATEGLERASARRELYSDAFLIKRPLLQAIDPARTRPAVLLIDEIDRADEEFEGYLLEL
                     LAEFQITIPELGTVRAKEPPLVILTSNRTREVHDALKRRCLYQWIEYPSFAKEVEIVR
                     ARVPQAAAQLARQVTALVQELRSVELYKVPGVSETLDWVAAIATLDRRALDIQTVEET
                     IGVVLKAKEDIDALRGERLAGLLERAMAR"
     gene            2511..3683
                     /locus_tag="AC16CY_17150"
     CDS             2511..3683
                     /locus_tag="AC16CY_17150"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3355581.1"
                     /translation="MTTFTDNLLSFGRLLRRSGFEVPVGNVLDLTGALTLVNLAVRDE
                     VFHACRTVLVRRHDQLAAFEKIFDAFWRQHGNPFASPATNTTRDAQAAAASVPVAAAA
                     IHDGELSNEDQVGVEEPTAGGLQTWSDAAALARKDFAEFTAEEVAEARAFIDRLAWIP
                     GERRTRRWIRGRGSRADLRRALSRSLRTGGDVLVIPTRRRRVRLRPLVLLCDVSGSME
                     RYSRMLLHFAHRVAHQHGRLEVFLFSTTLTRVTRQLRIRKLDAAAAAVSDAVPDWSGG
                     TRIGPALQQFHRRWSRRVLHEGPVVLLISDGWDRGDPKVLRDEIARLQRSCHRLIWLN
                     PLIGTVDYAPLTRGLQAALPFVDDFLPARTLTDFKELALHLNAVAADSRRRRLKDS"
     gene            3683..4114
                     /locus_tag="AC16CY_17155"
     CDS             3683..4114
                     /locus_tag="AC16CY_17155"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3355582.1"
                     /translation="MNISGTYSFDAPPPQVWDLLMDPEVISSCIPGCEKLVPDGEDRY
                     RAKLTVALAAICGTYDGTVTISDKVTHASYRLTVEGQGPPGFVKGQAAIALRPEAAGT
                     VVEVTGDVQTGGPIARVGQRLISSVSKMMQDRFFACLQGKV"
     gene            complement(4118..>4931)
                     /locus_tag="AC16CY_17160"
     CDS             complement(4118..>4931)
                     /locus_tag="AC16CY_17160"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3355583.1"
                     /translation="IEEPKYVLGATTREQERLLMQCKLLEPQARWMLEHIGVGRHWNT
                     VDVGCGPLGILDLLVEYTGPGTEVIGVERDPRMLSFGRSLMAERGLDSVKYVDGDARD
                     TRLPSSSFDLAHARLLLVNLPEPEGVVAEMVDLVRPGGWVAVQEVDWISWTCDPIHPS
                     WTRLIEINAAIWGARGMDVNIGRRLPRMLTQAGLTDIQCKTHTPVYSNKDQYQYLLLD
                     FSRINKTEMIESGYTTEQEWTELTESLRAHLSHPETFVTWSLFCQAWGRKPN"
BASE COUNT          784 a         1592 c         1652 g          903 t
ORIGIN      
        1 cttgggagtt gggcgttgga agttgggagt tgacgcgcat cgtgctcagt aggtcaccct
       61 cacgcggtac ctcagcgtcg cttctccgtc cgacgccacc ggcacgctga actgcgccgc
      121 ccatgcgtct gtcttcgtcc actggtggct ggatcgcagc atccgccagg tgcccccgat
      181 tggctcgttg acctcgaccg acacggccgt ggttttgtgg ttgcgcagga cgatttcgaa
      241 ctccatctcg tagacgttcg acgtgatttt ctcgaagtcg atctgcttgc gggtcgccac
      301 gacgtcgaag gcgtggccga tcttcacgtt gagcgtctcg tccttcggcg tgtggtcgat
      361 gcggtcctcg ccgacaaact gcacgccgcc cttcgagtcg gcctgataca cgcggacgac
      421 gcctgaaggc atcggcagtc caaggccggc cttctcttcg ttcttgaact ggtagtagac
      481 cttgaccacg tccttgagcg gcgaaccggg atgctgggcg ttgcggtagt agaacgcctg
      541 cccgtccacg atgtagcgct tgaggatcgg gaagtcgctg gcgccgagca tgctgacctg
      601 cttggtctgg ttgttgttga tcgtcgtctt gcggtcgagc gtgtagaggt ggtagtcgcc
      661 gaacgattcc tgcgtcactt ctcgagcggc cgcagcgtcc tgtctggcga agcgttcggc
      721 cagctccatg cgcccgacgg ccgactgcac gcggttcaag tcgccggcga cgagctgcag
      781 cctggcgttc ctgaacgccg ttccgctgcc gttgttgacc gtgacccagc cgtcgatgtc
      841 ggcggccttg tcgtcgcgcc cgaccgtgag cacgtagtcg gcgttccacg tcaggttggt
      901 cgccaggtac gacgcctcga cgcggtgacg ttggccgccg tcgttgttga gcgaccagat
      961 cagcgtgggc cgtgagtaga ggttgccggg aacttccggg aagcggatgt gatcggcgcc
     1021 gagcccggtg acgatctcct tgccgatctg ccagaccgga gcctggttgt agctcaggag
     1081 cctggccgtg accggctctt cgatcgtcgc gcccccttcc tgccgccgcc gcacgagcgt
     1141 gacatcccgg ccgacgtact tgcgaagaag cttgtccggt tcgagcagat cgtactcgta
     1201 gttctgctcg aggacgctga cccgccccgg ctcgctgagc gaacggaagt gaacggtggc
     1261 cggattcacc gtggcggcga tgtccatgaa ctgcaggtcg aagaccccgc gcggcacttg
     1321 cagattgcgg acgtcccgga ccagcgcgat gttcgagttg tacaccgtca gcgagagctc
     1381 ggtctggtcg tcgagggtgg acgacccctg cgcgggggcg gagggcgggg ccgccgcgac
     1441 gggcgcagga tcgttgagaa gggtcgcgag gatgccggcg gcggccaggg ccggcgccgc
     1501 aagccagaga cgtgcagaca tgtgtaaacc cctttccagg cgttccggcc ggcaggccgg
     1561 gaccagcccg cattccgggc tgatatcctt ggaacggtgc tgtcgggaaa ccttgcaggg
     1621 gacggggtcg cgtccatttc cgacctcgaa cagcggctca cggcgcggcg ctacgtcgcg
     1681 gaccgcggcc tcgccgtgtc tttgtatctg gcccttcgtt tgaggaggcc gctttttctc
     1741 gagggagagg ccggtgtcgg caagacgtct cttgcgacgg ccgtcgcgga agcgatcgac
     1801 gcagacctga ttcggttgca gtgctacgag ggcctggacg tcacccacgc ggtgtacgag
     1861 tgggactacg cccgtcagct cctcgagctg cgaatcctcg aagccaccga agggctggag
     1921 cgtgcgtccg cccggcgcga gctgtacagc gatgccttcc tcatcaagcg tccgctcctg
     1981 caggcgatcg accccgcgcg cacgcgtcca gccgtcctgc tgatcgacga gatcgatcgg
     2041 gccgacgagg agttcgaagg gtatctgctg gagctgctgg ccgagttcca gatcacgatt
     2101 cccgagctcg ggacggtgcg ggccaaggag ccgcccctcg tcatcctcac gtccaaccgt
     2161 acgcgggagg tgcacgacgc gctcaagcgg cgatgcctct accagtggat cgagtacccg
     2221 tccttcgcca aagaggtcga gatcgtccgt gcccgggtgc cgcaggcggc cgcgcagctg
     2281 gcccggcagg tgaccgcgct ggtccaggag ctgcgcagcg tcgagctgta caaggtgccc
     2341 ggcgtctccg aaacgctcga ctgggtcgcg gcgatcgcca cgctcgatcg acgcgcgctc
     2401 gacattcaga cggtggaaga gaccatcggc gtggtgctca aggccaagga ggatatcgac
     2461 gcactgcgcg gggaacggct cgccggactg ctcgaacgcg cgatggcacg atgacgacgt
     2521 tcacggacaa cctgctgtcg ttcggccggt tgcttcggcg cagcgggttc gaggtgcctg
     2581 ttggaaacgt tctcgacctg accggggcgc tcaccctggt gaacctcgct gttcgcgacg
     2641 aggtgtttca cgcctgccgc acggtcctgg tgcgccggca cgatcagctc gcggcgttcg
     2701 aaaagatctt cgacgcgttc tggcggcagc acggcaatcc gttcgcgtcg ccggcgacca
     2761 acaccacccg tgacgcgcaa gccgccgccg cgtcggtccc cgtggcggcc gccgcgatac
     2821 acgacggtga gctgagcaac gaagaccagg tcggggtcga ggaaccgact gcgggcggac
     2881 tccagacatg gagtgacgcc gcagccctgg ctcgaaaaga cttcgccgag ttcacggcag
     2941 aggaagtcgc cgaggcacgt gcgttcatcg atcggctcgc gtggatcccc ggcgagcgcc
     3001 ggacccggcg ttggatccgg ggcaggggtt cgagagcaga cctgcggcgc gcgctctctc
     3061 gaagcctgcg cacgggcggc gacgtccttg tcatccccac gcgacgccgg cgggtccgtc
     3121 tccgtccgct cgtcctgctc tgcgacgtca gcggctcgat ggagcgctac tcgcgaatgc
     3181 tgctgcactt tgcgcacagg gtggcgcacc agcacggccg tctcgaggtg ttcctgttct
     3241 cgacgacgct gacgcgcgtg acgaggcagc ttcgcatccg caagctcgat gcggccgccg
     3301 cggccgtgtc cgacgccgtg cctgactggt cgggtggtac ccgcatcgga ccggcgctcc
     3361 agcagtttca tcgacggtgg tcgcgccgcg tgctccacga agggccggtc gtgctgctca
     3421 tttccgacgg ctgggaccgc ggcgacccga aggtcctgcg cgacgagatc gcccggcttc
     3481 agcgcagctg ccaccggctc atctggctca atccgctcat cggcaccgtg gactacgccc
     3541 cgctcacacg aggcctgcag gcggcgcttc cgttcgtaga cgatttcctt cccgcacgca
     3601 cgctcaccga tttcaaggag ctggcgctac acttgaacgc tgttgccgcc gacagtcgtc
     3661 gtcgccggct caaagacagc tgatgaacat ctccggcacc tactcattcg acgctccacc
     3721 tccgcaggtg tgggacctcc tgatggatcc ggaagtcatc tcgtcgtgta ttccgggttg
     3781 cgagaagctc gtgcccgacg gcgaggaccg ataccgcgcc aagctcaccg tcgcgctcgc
     3841 ggcgatatgc ggcacatacg acggcactgt caccatctcg gacaaggtga ctcacgcgtc
     3901 gtaccgtttg acggtggagg gtcaaggccc gccggggttc gtaaaggggc aggccgcgat
     3961 tgcccttcgt cccgaggctg ccggcaccgt cgtggaagtc acgggcgatg tgcagacggg
     4021 cgggccgatt gcgcgagtgg gtcagcggct gatctcgagc gtgtcgaaaa tgatgcagga
     4081 cagattcttt gcctgcctgc agggaaaggt ctgagcgtca gttcggcttg cggccccagg
     4141 cctggcagaa cagcgaccag gtgacgaacg tctccgggtg ggacaggtga gcgcgtagcg
     4201 actcggtcag ctccgtccac tcctgctcgg tggtgtagcc gctttcgatc atttcagtct
     4261 tgttgattct ggagaagtcc agcagcaggt actggtattg atctttgttg ctgtacacag
     4321 gcgtatgcgt cttgcactgg atgtcggtca gcccggcctg tgtgagcatg cgcggcagac
     4381 ggcggccgat gttcacgtcc atgccgcgcg cgccccagat cgccgcattg atctcgatga
     4441 gacgtgtcca ggagggatgg atcggatcgc aggtccagga gatccaatcg acctcctgca
     4501 ccgccaccca gccgcccggc cggaccagat cgaccatctc ggcgacgaca ccttcgggtt
     4561 cgggcaagtt gacgagaagc agccgggcgt gtgcgaggtc gaaactcgag gacggcagcc
     4621 gcgtgtcccg cgcgtcgccg tcgacgtact tcactgaatc gagcccgcgc tcggccatca
     4681 agctgcggcc gaacgagagc atgcgaggat cgcgctcgac accgatgact tcggtgccgg
     4741 gcccggtgta ctccaccaga agatcgagga tgccgagggg cccgcaaccc acgtcgaccg
     4801 tgttccagtg cctgcccacg ccgatgtgct ccagcatcca gcgggcctgg ggctcgagga
     4861 gcttgcactg catcagcaga cgttcctgct cgcgtgtggt cgccccgagc acgtacttgg
     4921 gttcttcaat c
//
LOCUS       DBNGSH010000747         1996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3987687, whole genome shotgun sequence.
ACCESSION   DBNGSH010000747 DBNGSH010000000
VERSION     DBNGSH010000747.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3987687"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1352
                     /gene="ligM"
                     /locus_tag="AC16CY_17165"
     CDS             3..1352
                     /gene="ligM"
                     /locus_tag="AC16CY_17165"
                     /EC_number="2.1.1.341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046274 - lignin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanillate/3-O-methylgallate O-demethylase"
                     /protein_id="HIR3355584.1"
                     /translation="MQAVGSPVKLLRNSQTGPYIYPVVPSEFSNWRDEQRAWRETCIL
                     FNQSYHMTDMIVEGPDAPRLLAALGVNSFKGFEPDKAKQFVACNHDGYVIGDVVLFHL
                     ERNRFNLVGRPSVHNWVQYHCETGGYDARVERDERTVARPGPVVRKTYRFQVQGPRAL
                     DVVSKVSGAPVPDVKFFNMTAFTIAGKHVRALRHGMVGQPGFELFGPWQDGEEVKAAI
                     IEAGREFGLRQSGARTYSSNTLESGWIPSPLPAIYTGDKMKAYRQWLPANGYEASASL
                     GGSFYSDNIEDYYLTPWDLGYGSIVKFDHEFIGREALEKIAARPRRKKVTLVLNGEDV
                     TRAIGTMFQRENRTKYFDFPSSVYSTLPYDKVQRNGKTVGVSTWCGYSANEGAMLTLA
                     MVDVEHSEPGTDVVFVWGEEGGGSSKPTVERHVQVEIRATVAPVPYVDVVRAAYRSN"
     gene            complement(1370..1699)
                     /locus_tag="AC16CY_17170"
     CDS             complement(1370..1699)
                     /locus_tag="AC16CY_17170"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3355585.1"
                     /translation="MPTPPLTLLLNQRGLDVNLPPPAPREAAVPRPQIVVEYASDKRL
                     SINTQPVALGDLGDRLRQLYEDRTDKTMYVMGAGSLRYADIVSVIDAAKGAGVTRVGI
                     VTESMRR"
     gene            complement(1722..>1996)
                     /locus_tag="AC16CY_17175"
     CDS             complement(1722..>1996)
                     /locus_tag="AC16CY_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355586.1"
                     /translation="AKFPSLTPFLVWLTKFHNPPLFTLLFGLHFTYYIFLFERVSLYQ
                     FLVHVKNFAINPFLRRKQPMASTGRPYTPPPSPPSSGSTPRTIPVN"
BASE COUNT          365 a          605 c          699 g          327 t
ORIGIN      
        1 tgctgcaggc cgtcgggagc ccggtgaagc tgctgcgcaa ctcgcagacc gggccgtaca
       61 tctaccccgt ggtcccgagc gagttctcga attggagaga cgagcagcgc gcctggcggg
      121 agacgtgcat cctcttcaat cagtcgtacc acatgacgga catgatcgtc gaagggcccg
      181 acgcgcccag gctgctcgcc gcgctcggcg tgaacagctt caagggcttc gagccggaca
      241 aggcgaagca gttcgtcgcg tgcaatcacg acggctacgt gattggcgac gtcgtgctct
      301 ttcacctgga gaggaaccgg ttcaacctgg tcggccgtcc ctcggttcac aactgggtcc
      361 agtaccactg cgaaacgggg gggtacgacg cgcgcgtgga gcgggacgag agaaccgtcg
      421 cccggcccgg tcccgtcgtc cggaagacct accggttcca ggtccagggt cctcgcgcac
      481 tcgacgtcgt gtcgaaggtg tcgggggcgc cggtgcccga cgtcaagttc ttcaacatga
      541 cggcgttcac gatcgcgggc aagcacgtcc gcgcgctccg gcacggcatg gtgggccagc
      601 ctggattcga gctgttcgga ccgtggcagg atggcgaaga agtgaaggcg gccatcatcg
      661 aagccggacg cgagttcggg ctccggcaat cgggcgcccg gacctattcg agcaacacgc
      721 tggaatcggg ctggatcccc tcgccgctgc ctgccatcta caccggcgac aagatgaagg
      781 cgtaccggca gtggctgccg gccaacggtt acgaagcctc cgcctcgctc ggtgggagct
      841 tctactcgga caacatcgag gactactacc tgacgccctg ggacctgggc tatggctcga
      901 tcgtcaagtt cgaccacgag ttcatcggcc gggaggccct cgagaagatc gcggccaggc
      961 cgaggcggaa gaaggtcacg ctggtgctca acggcgagga cgtcacgcgc gccatcggca
     1021 cgatgttcca gagagagaac agaaccaagt acttcgactt cccgtcctcc gtgtactcga
     1081 cgctgcccta cgacaaggtg cagaggaacg gcaagacggt gggcgtttcg acgtggtgcg
     1141 gctacagcgc caatgaaggc gcgatgctca cgctggcgat ggtcgacgtc gagcacagcg
     1201 agcctggcac cgatgtcgtc ttcgtgtggg gcgaggaggg cggcgggtcg tcgaaaccga
     1261 ccgtcgagcg ccacgtccag gtggagatca gggcgacggt cgcgccggtc ccgtacgtgg
     1321 acgtcgtgcg cgcggcgtac cggtcgaatt gagctgaggg acagcggggc tagcgacgca
     1381 tgctctccgt cacgatgccg acgcgcgtca cgccggcacc ttttgcggcg tcgatgaccg
     1441 agacgatgtc ggcgtagcgg aggctgccgg cgcccatcac gtacattgtc ttgtcggtgc
     1501 ggtcttcgta gagctgtcgc aggcggtcgc caaggtcacc gagggcgacc ggctgcgtgt
     1561 tgatcgacag gcgcttgtcg ctcgcatact caacgacaat ctgcggtctc ggaacggctg
     1621 cctcgcgggg ggccggcggc ggcagattca cgtcaaggcc gcgctggttc agcagcagcg
     1681 tcagaggtgg cgtaggcacg agccagttcg tcgtcggccg atcagttgac gggaatggtc
     1741 cggggcgtcg atccgctcga cggcggactc ggaggcggcg tgtacggccg tccggtgctc
     1801 gccatcggct gcttcctgcg caggaacggg ttgatggcga agttcttcac gtgcaccagg
     1861 aactggtaga gcgacacccg ctcgaacagg aagatgtaat acgtgaagtg caacccgaac
     1921 aggagcgtga agagcggcgg gttgtggaac ttcgtcagcc acaccaggaa gggcgtgagc
     1981 gacgggaact tcgcgc
//
LOCUS       DBNGSH010000748         5048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2291221, whole genome shotgun sequence.
ACCESSION   DBNGSH010000748 DBNGSH010000000
VERSION     DBNGSH010000748.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2291221"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /gene="lpxA"
                     /locus_tag="AC16CY_17180"
     CDS             complement(<1..1187)
                     /gene="lpxA"
                     /locus_tag="AC16CY_17180"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR3355587.1"
                     /translation="MALDIPALLGRVYYRYPSAMFDAVTEHEPGRRLVAVKNVTFNEE
                     FFQGHFPGMPLMPAVLMIESLTQVAAVLVLERTNAPQASRASLRGVDGAKFRRTVVPG
                     DRMRLEVSLVRLRSRLARVRAAAYVADDVVVEADLLLAIEPGPAFVDPSATVHPAARI
                     GVGTTIGPHAIIGPDVRVGRHCTIGASAVIDGRTEIGDETQIFPMASIGLAPQDLKYK
                     GEPTALKIGRRNVFREFVTINRGTAGGGGETTIGDNNLFMAYVHVAHDCHVGSHTIFG
                     PHATMGGHVSVEDFANISAGSAVHQFCRVGRYGFIGGYSVVTKDALPFGRTIGSRPAR
                     IFGVNTIGLSRRGFTPDTIAQLRHAYRYLLQSKLNTTRALAQIEQDTSLSTPEVRYLV
                     DFIR"
     gene            complement(1220..1936)
                     /locus_tag="AC16CY_17185"
     CDS             complement(1220..1936)
                     /locus_tag="AC16CY_17185"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3355588.1"
                     /translation="MLAFVLVLNTAVAVGMAQAQASQAPAPKPTAPAPKPAAPAPAQP
                     AQAQLPPPPKPRFQEGLKYAWVNPQQVAAYSAVGKVAAEKINALRDQKTRELQDRQKT
                     LQANQQRLERDGGVMNDQARAQLQAEIEKGQRDLQRLTEDAQQEMQTLAEQVEADFNR
                     KLFPVIERVAGQKGVHFVFNGAQSGLIWAAQGMDLTAEVIQALDSPPPAAATTPAPTT
                     PAAGAPAPAGAPTAPATTPR"
     gene            2009..2470
                     /locus_tag="AC16CY_17190"
     CDS             2009..2470
                     /locus_tag="AC16CY_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355589.1"
                     /translation="MVCASHPALETFLSMRYFTATIVLLPLTAALAAGCSSSNNTPTA
                     PDGPDPIAIVETFPESLNVNGARTHVFRVDTVGTVTARIATLSDPSAIVGLSMGTWNG
                     AACQILLANDAAAMNVTLTGTAQSVGQFCARVYDVGRLTATVDYSLEVAHY"
     gene            complement(2490..>5048)
                     /locus_tag="AC16CY_17195"
     CDS             complement(2490..>5048)
                     /locus_tag="AC16CY_17195"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/OMP85 family outer membrane protein"
                     /protein_id="HIR3355590.1"
                     /translation="FVPYDQKTEETMLSDFKTLWATNFLDDLSMEITEHKFPNGVIGK
                     MIVYHMEERERIKIVDYRDRDGESIKIIKRTDIDTKLRERLIEMRLDAFRDDGTIRRV
                     EGVLREMMAEKGFTNAQVDHTVTPVAGGPKLVNVTFVVGEGPKVKIRDLEFIGNEAIS
                     DSALQRKIKENKPKNPFYGWISGGGTFNEAMFEEDAQRVVEHYHNEGYPQARVGQPEL
                     KVLEDTKDGKTQWIQLRIPVTEGKRYKFDQLDFEGNRLIRSEGLKTLYTIKPGDWYSR
                     KKIVEGNEKARSAYGSRGYMEFVPAPILAYSDDPNSPERRLASQVPAALAAPQETPAA
                     TAAGTADAPTVDITISVNEGPQYFVNRISFTGNTTTRDNVIRREMRLLEGGVFDMEAL
                     KYSVRRLNQLGYFKELKGDDSDMKVEKTPGQENVVDVTLNFEEQNRNQLTFGAGVSQY
                     EGFFGQLGFQTSNFLGRGESLTLSLQAGVRAQNYQLAFTEPFLFERNLTGGFDLFNRS
                     LQYIGYYTQKSRGGNVFLGFPVADFSRMLIGYSYQTVKITDLNEALFDQSCIVRPTGC
                     TTFASAADLSRLTQAQIDVLRRNPFIYDSLLVGAGGHRTISKVTPSFIHNTVDHPIFP
                     NAGKRLTASIDLAVLGGNTNYYKPRVEWIGFYKHLSRTSFGFRAQAEFIAPVGDVQSL
                     PVFERLFLGGEYSVRGFDIRSIGPSVPGSFVVLGGNKSLLFNAEYMVSIMSQVRIVAF
                     YDAGQVRDFGEPFAWTEPLLEVRTPTPPALIDPFAPFSALVAPGATGVQTIPIGETSA
                     FKTSTGVELRFFMPVLNVPFRLIYAWNPQRGGVLDNNLQPAKAQTFRFAVGTTF"
BASE COUNT          802 a         1681 c         1629 g          936 t
ORIGIN      
        1 cggatgaagt cgacgaggta gcgcacctcc ggcgtcgaga gcgacgtatc ctgctcgatc
       61 tgcgcgagcg cccgcgtggt gttcagcttc gactggagga ggtagcgata ggcgtggcga
      121 agctgcgcga ttgtgtcagg cgtgaacccg cggcgggaca gcccgatcgt gttcacgccg
      181 aaaatccgcg cgggacggct cccgatcgtg cgcccgaacg gcagcgcgtc cttggtcact
      241 accgagtagc cgccgatgaa gccgtatcga ccgacgcggc agaactggtg cacggccgac
      301 cccgcgctga tgttggcgaa gtcctccacg ctgacgtggc cgcccattgt ggcgtgaggc
      361 ccgaagatcg tatggctgcc gacgtggcag tcgtgggcca cgtggacgta cgccatgaag
      421 aggttgttgt caccaatcgt cgtctcgccc ccgcctccgg ccgtcccgcg gttgatggtg
      481 acgaattcgc ggaacacgtt gcgccggccg attttgaggg cggtcggctc gcccttgtac
      541 ttgagatcct gtggcgccag cccgatcgac gccatgggaa agatctgcgt ctcgtcccca
      601 atctcggtcc ggccgtcgat cacggccgat gcgccgatcg tgcagtgccg tcccacgcga
      661 acgtccgggc cgatgatggc gtgcggcccg atggtcgtgc ccactccaat ccgggccgcg
      721 gggtgaacgg tcgccgaggg atcgacaaac gccggccccg gctcgatggc gagcagcagg
      781 tccgcttcga ccaccacgtc atccgcgacg tatgccgccg cccggacgcg cgccaggcgc
      841 gagcgcagcc ggacgagcga cacctcgagc cgcattcgat ccccgggcac caccgtccgg
      901 cgaaacttcg cgccgtccac gccccgcagg gaggcgcgcg acgcctgcgg cgcgttcgtg
      961 cgctcgagga ccagtacagc ggccacctgc gtcagcgatt cgatcatcaa caccgccggc
     1021 atgagcggca tgccagggaa atgaccctgg aagaactctt cgttgaaggt cacgttcttc
     1081 acagccacga gccggcgccc cggctcgtgc tcggtgacgg cgtcgaacat ggccgacgga
     1141 tagcggtagt aaacgcgtcc gaggagtgcg ggaatgtcga gggccacgaa tcggcctgag
     1201 agaccgaaaa cgccgcgcct tagcgcggcg tcgtcgcggg cgcggtcggc gccccagctg
     1261 gcgccggagc cccggcggcc ggtgtcgtcg gcgcgggcgt ggttgccgct gccggtggag
     1321 gcgagtcgag cgcctgaatg acttcggcag tcaggtccat gccctgcgcg gcccagataa
     1381 gtccgctctg cgcgccgttg aacacgaagt gcaccccctt ctgtcccgcc accctttcga
     1441 tcaccgggaa cagcttccgg ttgaaatcgg cttccacctg ctcggcgagc gtctgcattt
     1501 cctgctgcgc atcctcggtg agccgctgca gatctcgctg ccccttctcg atctcggcct
     1561 gcagctgggc acgcgcctga tcgttcatca cgccgccgtc ccgttccagc ctctgctggt
     1621 tggcctgcag cgtcttttgc ctgtcctgca gctcgcgcgt cttctgatcc cgcagcgcgt
     1681 tgatcttctc ggcagccacc ttgccgacgg ccgagtaggc cgccacctgc tgggggttga
     1741 cccacgcata cttgaggcct tcctgaaagc gcggcttggg cgggggcggc agttgcgcct
     1801 gcgctggctg tgcgggcgcc ggcgcggcgg gcttgggcgc gggggccgtg ggtttcggcg
     1861 ccggagcctg tgaggcctgg gcctgcgcca taccgacggc gacggccgtg ttcagcacca
     1921 gaacgaaggc taacactctc attcctctcg gtcctttctc gtctgctgga acccgacgat
     1981 tttactcgac catcggacgc caagtaagat ggtctgcgcc agccaccctg ccctggagac
     2041 cttcctgtcg atgcgctact tcaccgccac gatcgtcctg cttccgctga cagccgcctt
     2101 ggccgcggga tgcagcagca gcaacaacac acccacggcc cctgacgggc ccgacccgat
     2161 cgcgatcgtc gaaacgttcc cagagtccct caacgtgaac ggcgcccgga cccatgtgtt
     2221 cagggtcgat accgtcggca cggtcacggc ccgaatcgcg acgctgtcgg acccgtccgc
     2281 tatcgtgggg ctcagcatgg gcacgtggaa cggcgccgcc tgccagatcc tcctcgccaa
     2341 cgacgccgcc gcgatgaacg tcaccctcac aggcacggct cagtccgtgg ggcagttctg
     2401 cgcgcgcgtc tacgacgtgg gcaggctgac agcgaccgtg gactactcgc tcgaggtcgc
     2461 gcactactga cctcgaactg ccagctgcct cagaacgtcg tccccacggc gaacctgaac
     2521 gtctgcgcct tggccggctg gaggttgttg tccagcacgc ctccacgctg cgggttccac
     2581 gcgtagatca gccggaacgg cacgttcagg acgggcatga agaagcggag ctcgacgccg
     2641 gtggacgtct tgaacgcgct cgtctcccca atcgggatcg tctgcacgcc tgtggcgccg
     2701 ggagcgacga gcgcggagaa gggcgcgaac ggatcgatca acgccggcgg cgtcggcgtc
     2761 cgcacctcga gcagcggctc ggtccacgcg aacggctcgc cgaagtcccg cacctggccc
     2821 gcgtcgtaga acgccacgat gcgcacctgg ctcatgatcg agaccatgta ctcagcgttg
     2881 aaaaggaggc tcttgttgcc gcccagcacg acgaacgacc ccggaaccga cgggccgatg
     2941 gatcggatgt cgaagccgcg cacgctgtac tccccgccga ggaacaaccg ctcgaacact
     3001 ggcagcgact ggacatctcc gaccggggcg atgaactctg cctgggcgcg gaacccgaag
     3061 gacgtgcgcg acaggtgctt gtagaagccg atccattcca cccgcggctt gtagtagttg
     3121 gtattgccgc caagcacggc gaggtcgatc gaggccgtca gtcgcttgcc cgcattcggg
     3181 aagatcgggt gatcgacggt gttgtggatg aagctcggcg tgaccttgct gatcgtgcga
     3241 tggccgcccg ccccgacgag cagcgaatcg tagatgaacg gattgcgccg cagcacgtcg
     3301 atctgcgcct gtgtcagccg tgacaggtcc gcggccgagg cgaaagtggt gcatcccgtc
     3361 ggacgcacga tgcaggactg gtcgaacaac gcctcgttca ggtcggtgat cttcaccgtc
     3421 tggtacgagt aaccgatcag catccggctg aaatcggcga cggggaagcc caggaacacg
     3481 ttcccgccgc gcgacttctg cgtgtagtag ccgatgtact gcagcgaccg gttgaacaga
     3541 tcgaacccgc ccgtcaggtt gcgctcgaac aggaacggct cggtgaacgc cagctgatag
     3601 ttctgcgccc ggacgcccgc ctgcagcgag agcgtgaggc tttcgccgcg gccgaggaag
     3661 ttggacgtct ggaacccgag ctggccgaag aagccttcgt attgcgacac gccggcgccg
     3721 aaggtgagct ggttgcggtt ctgctcctcg aagttcagcg tgacgtcgac gacgttctcc
     3781 tggccgggcg tcttctcgac cttcatgtcg ctgtcgtcgc ccttgagctc cttgaagtag
     3841 ccgagctggt tcaggcgccg gacgctgtac ttgagcgcct ccatgtcgaa gacgccgcct
     3901 tcgagcagcc gcatctcgcg gcggatcacg ttgtcgcgcg tcgtcgtgtt gcccgtgaac
     3961 gagatgcggt tgacgaaata ctgcggtccc tcgttgacgc tgatcgtgat gtctaccgtg
     4021 ggcgcgtcag cggtccccgc ggccgttgcg gccggcgtct cctggggcgc cgccagggcg
     4081 gccgggacct gcgacgccag cctgcgttcc gggctgttgg ggtcgtcgct gtaggcgaga
     4141 atcggcgcag gcacgaactc catgtagccc ctgctgccgt aggctgaccg tgccttctcg
     4201 ttgccctcca cgatcttctt gcggctgtac cagtccccgg gcttgatggt gtacagggtc
     4261 ttcagccctt cgctgcgaat cagcctgttc ccttcgaagt cgagctgatc gaacttgtat
     4321 cgcttccctt cggtcacggg gatgcgcagc tgaatccatt gcgtcttccc gtccttggtg
     4381 tcttcgagca ccttgagctc gggctggccc acacgggcct gcggatagcc ctcgttgtga
     4441 tagtgctcga cgacacgctg cgcgtcctct tcgaacatcg cctcgttgaa cgtgccgccg
     4501 cccgagatcc agccgtagaa ggggttcttg ggcttgttct ccttgatctt ccgctgcagc
     4561 gcgctgtcgg agatcgcctc gttgccgatg aactccaggt ctcgaatctt gaccttcggt
     4621 ccctcgccaa ccacgaacgt cacgttcacc agcttcggac cgcccgccac cggcgtgacc
     4681 gtgtgatcga cctgcgcgtt ggtgaacccc ttctccgcca tcatctcgcg cagcacgcct
     4741 tcgacacggc ggatggtgcc gtcgtcgcgg aaggcgtcga ggcgcatttc gatgaggcgc
     4801 tcgcgcagct tggtgtcgat gtcggtgcgc ttgatgatct tgatgctctc gccgtctcta
     4861 tcccgatagt cgacgatctt gatgcgctcg cgctcctcca tgtggtagac gatcatcttg
     4921 ccgatgacgc cgttcgggaa cttgtgttcg gtgatctcca tcgacaggtc gtcgaggaag
     4981 ttcgtcgccc agagcgtctt gaagtccgag agcatcgttt cctcggtctt ctggtcgtag
     5041 ggtacgaa
//
LOCUS       DBNGSH010000749         9025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_423036, whole genome shotgun sequence.
ACCESSION   DBNGSH010000749 DBNGSH010000000
VERSION     DBNGSH010000749.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_423036"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="AC16CY_17200"
     CDS             <1..360
                     /locus_tag="AC16CY_17200"
                     /inference="COORDINATES: protein motif:HMM:NF020977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR3355591.1"
                     /translation="AAVMNKGVEKGRDLEDAEILQVVSSLVKQRRDSIEQFEKGGRRD
                     LVEKETAEIAVLQAYLPPAASPEEIDAAVAAAIAETGASSPKDMGKVMKAVMPRLAGK
                     NADGKAVNEAVRRKLGA"
     gene            350..1948
                     /gene="murJ"
                     /locus_tag="AC16CY_17205"
     CDS             350..1948
                     /gene="murJ"
                     /locus_tag="AC16CY_17205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3355592.1"
                     /translation="MAPEPHSTSALARSAASSGAATMTSRILGLARDQVLAYQFGASA
                     AMDAYNVAFRVPNLVRDLFAEGAMSAAFVPTFTKHLTSSGKESAWRLGNNVVNALLVV
                     TGCLVVLGIIFAEPLVAAFTAERFTRTQLPLTVQLARIMLPALTLIALAAAFMGMLNS
                     LHHYFIPALSPAMFNVVTIASVFALVPILPAFGIEPITAVAIGTLLGGVAQLALQWPT
                     LRHEGFRYRPVLDWNDAGLRRVLALMGPGTAGLAATQVNIFVNQLLATGTVDGAVTWL
                     QYAFRVMYLPIGLFGVSIATAALPAVSRQHHLDDRQAVRGTVANSLSLMLMLNVPAMV
                     GLMVLAEPIVRVIFEHGEFTEVDTLATAAALRFYALGLLAYSVVRIVSPVFYALGRNR
                     TPVLVSIVTVLINAGLNVTLVRVLGYRGLALGTSIAALFNAAVLLTLLRGHLGGLQEG
                     RLASSFARVAAASAVMGVAASTADGALASVLPGDGVVLQIVRLASTIGIALVVLAGAS
                     WLLRIREFTESVSLVTKRLRRAGR"
     gene            2037..2816
                     /locus_tag="AC16CY_17210"
     CDS             2037..2816
                     /locus_tag="AC16CY_17210"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3355593.1"
                     /translation="MRGRYPQLSEIRYAFGPGPMTPAVKAILITNVMVFLVTYFANSS
                     PLEGNPVIRLLGLQPASLFGKFTIWQPITYMFVHGGVSHIFFNMLTLWLIGVELERLW
                     GTTYFTKFYFVSGLGAGFTQVLLGILPFDFANQFYLPITIGASGAIYGLLLAFALYYP
                     TRPFLVFFIFPVQARYFVMILGGLSLLFSLGGGSGVAHTAHLGGLVTGYLYLRRPRIT
                     PRINIFSEIQYRFLKWRINRMRRKFDVYSGGRRDDWDRRVH"
     gene            complement(2896..3243)
                     /locus_tag="AC16CY_17215"
     CDS             complement(2896..3243)
                     /locus_tag="AC16CY_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3355594.1"
                     /translation="MAARLHRWDEIALERVTEMLSQKIVTGDREMIAQTYLKRGALVP
                     LHSHESEQMTYVLQGALRFLVDGEEVIVREGEVLHVPSGTPHQAEALEDTFELDMFSP
                     IRKDWVSEPERET"
     gene            3312..5465
                     /locus_tag="AC16CY_17220"
     CDS             3312..5465
                     /locus_tag="AC16CY_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit alpha/beta"
                     /protein_id="HIR3355595.1"
                     /translation="MKGSGLATGAESRAAEHQGRSRAYEGLTRNELVDAYRLMLLSRK
                     IDDREIQLKNQTKAFFQISGAGHEAVLVAAGRHLRPGIDWVFPYYRDRALCLTIGVTP
                     LEMFLASVGSTDDPANAGRQMPSHWGHRRWNIPSQSSCVGTHCLHAVGSAEAGMLYGR
                     LPQIEDREDYYSPDEIVYVSIGDGGTSEGEFWESLNTASTRHLPVLYLIEDNGYAISV
                     PTDVQTPGGDIGRVLEGYPGLRTFRCDGTDYLSSYRTLRDAVDHVRERNGPALVHATV
                     IRPYSHSLSDDEKLYKTPGEREAEARRDPLTRMRQFLLAEDLASEQDLADMMASVENE
                     VKAAAEEAQRAAQPEASSAAHFVFSPDVDPASSQFSTEARPQGAPDTMVSAINATLKD
                     EMARNPRIIVFGQDVADVSHSEALTLVAGKGGVFKVTHGLQRTYGSDRVFNSPLAEAN
                     IVGRAVGMALRGLKPVVEIQFFDYIWPAFMQIRNELSMMRYRSGNHWSCPVVIRVPIG
                     GYLRGGAPYHSQSGVSIFAHTPGLRIVLPSNAQDAAGLLRTAIRCDDPVLFCEHKHLY
                     RQTYNKGAYPGPDYMVPFGRAAVPREGRDVVVFTWGALVQRSLVAAQQAEQLGVSVAV
                     VDLRSIVPYDWETIAAYTKKTSRVIVAHEDQLTGGFGAEIAARISEELFEHLDAPVKR
                     VAALDCPVAYSPVLEEAILPGTEDVLEAVRAIVAY"
     gene            5514..6533
                     /locus_tag="AC16CY_17225"
     CDS             5514..6533
                     /locus_tag="AC16CY_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3355596.1"
                     /translation="MTRINVIALSLLLVALAALPDAAPAVDVLRSSGGLPAHIVGAYE
                     EPVDFQQASDGTYFVFNRRGHTVHAVDAARQASRKVVVLGGEEGRIIQPSGFDVTPDG
                     RIVVADMPRSQQRVQTFDAQGARLSGFFLPGRPVARVTIDNVMLNGAGSVQHTGSTLL
                     ISHPESGALVTEYSPGGYAQRSMGRLRPTGFEEDRELHVALNAGLPLVTPDGGFYYVF
                     VTGTPIFRKYDAKGVLQFERHVEGRELDASLAAQPTRWPRRKVEGDEVPFVAPIIRAA
                     AVNERGQLWISLTVPYTYVYDADGDKIRTVQFRAAGLISPISLSFSPSGRLLVTPGCY
                     EFDPR"
     gene            6647..6994
                     /locus_tag="AC16CY_17230"
     CDS             6647..6994
                     /locus_tag="AC16CY_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3355597.1"
                     /translation="MTPDKRDVLYGTLGLMVIKTLEALGPLHGYRIARRIEQISGNQL
                     SLNQGTLYPALLKLEEMGWISSKWGTSESGRRVRIYALTRAGRKQLQNEEAQWQRAAS
                     IVERFLKISQNPA"
     gene            6991..>9025
                     /locus_tag="AC16CY_17235"
     CDS             6991..>9025
                     /locus_tag="AC16CY_17235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3355598.1"
                     /translation="MTRLRAVLFRLRALFRSRQMDREIDEEITSHLAEATEEYVQQGL
                     SPDEARRAAQRRFGGVTQTKEVYREVWSIRWLEDLALDVRHALRTLRKSPAFTTAAAA
                     TLATAIGTNTAMFSVLNAVLLRPLPYRSPEQLAMLWTEDPTQNLREGRSALWDVEQWR
                     SHSQSVADMATFDSVSTVLTGADGAEQIAGASVSPNLLPLLGVRPVLGRSFSAEEAEQ
                     RQQLVLISHRFWQARFGGSHDAIGATLVLNGFPSRIVGILPADFQIANFDADVWGSHT
                     TRRSVRGPQTWFVIARLRPGATFEQAQVEMSAVARRLNDPLPAAERNRGISVVPLSLY
                     VVGPQSRLALWLLGGAVFFVFLIAAANVAGLSLARGTARTREMAVRAALGASAGRIAR
                     QLLTESVVLAIAGGLMGTLLALGGIRLIRAFGPGNLPRLNDVSLDLRVLGWALAVSLL
                     AGILVGLAPAITAMRRDLRPSGEESGRSVSGGTSSRRIRRGLVVAEFALAIVLLVGAG
                     LLVRSWWYVNNIDLGFTPERVLVMGVSTPTTFNDPAQRARLYHRVLEQIQAVPGVESA
                     GITGDLFVSNPRERVLTIDRDDGTVSERLPFARDEVSPDFFRTLGTPLLRGRFFTIGD
                     QPGTPPVAIINDAMARRSWPGQDPVGRRFMLGPRDSGGPWYAVVGVVADMRRQG"
BASE COUNT         1419 a         3041 c         2996 g         1569 t
ORIGIN      
        1 gcggcggtca tgaacaaggg cgtcgagaag gggcgcgatc tggaggacgc cgagatcctg
       61 caggtggtgt cgtcgctggt gaaacagcgc cgtgactcga tcgagcagtt cgagaaaggc
      121 gggcgccggg acctcgtgga gaaggagacc gccgagatcg ccgtcctgca ggcctacctc
      181 ccgccggcgg cctcccccga ggagatcgac gccgcggtcg ccgcggcgat cgccgagacg
      241 ggcgcgtcct cgcccaaaga catgggcaag gtgatgaagg ccgtcatgcc gaggctcgcc
      301 ggcaagaacg ccgacgggaa ggccgtcaac gaagccgtcc gccgcaagct tggcgcctga
      361 gccgcattcc acgtcggccc tcgcgcggtc cgccgcatca tccggcgcgg ccacgatgac
      421 gagccgcatc ctcgggctcg cccgcgacca ggtgctggcg taccagttcg gcgccagcgc
      481 ggcgatggac gcctacaacg tcgcgttccg cgtcccgaac cttgtcaggg atctcttcgc
      541 cgaaggcgcg atgagcgccg cattcgtccc gacgttcacg aagcacctga cctcgtccgg
      601 caaggaatcg gcgtggcggc tcggcaacaa cgtcgtcaac gcgctgctcg tcgtcaccgg
      661 gtgcctcgtg gtgctcggca tcatcttcgc cgaaccgctg gtcgcggcgt tcacggcaga
      721 gcgcttcacg cgcacgcaac tgccgctcac cgtgcagctc gcgcggatca tgctgcccgc
      781 cctcacgctc atcgcgctgg ccgccgcgtt catggggatg ctgaactcgc tgcaccacta
      841 cttcatcccc gcgctctcgc cggcgatgtt caacgtcgtc acgattgcga gtgtgttcgc
      901 gctcgtcccg atcctgccgg cattcggcat cgaaccaatc acagccgtcg ccatcggaac
      961 gctgcttggc ggggtggcgc agctcgcgct gcagtggccg acactccgtc acgagggctt
     1021 ccggtaccgg ccggtgctcg actggaacga tgcggggctg cggcgggttc tcgcgctgat
     1081 gggtcccggg accgcaggcc tcgccgcgac gcaggtcaac atcttcgtca accagttgct
     1141 ggcgacgggt acggtggacg gcgccgtcac gtggctgcag tacgcgtttc gcgtgatgta
     1201 cctgccgatc gggctgttcg gcgtgtccat cgcgacggcg gcgcttccgg ccgtgtcgcg
     1261 ccagcaccac ttggacgatc gacaggccgt gcgcggcacc gtcgccaaca gcctgtcgct
     1321 catgctgatg ctgaatgtgc cggcgatggt gggcctgatg gtgctcgccg agccgatcgt
     1381 ccgcgtgatc ttcgagcacg gagagttcac ggaagtggac acgctcgcga ctgccgcggc
     1441 gctccggttc tacgcgctcg gactgctcgc ctactcggtg gttcgcatcg tgtctcccgt
     1501 gttctacgcg ttgggtcgga accgcactcc ggtcctcgtg agcatcgtca cggtcctgat
     1561 caatgccggc ctgaacgtca ccctcgtccg cgtgctcggc tatcggggac tggccctggg
     1621 gacgtcgatt gcggcgttgt tcaacgcggc cgtgctcctg acgctgctgc gcggtcatct
     1681 cggcgggctg caggaagggc gcctcgcgag ctcgttcgca cgggtggccg ccgcctcggc
     1741 ggtcatgggc gtggcggcga gcaccgccga tggcgcactc gcgtcggtgc tgccgggcga
     1801 cggcgtggtg cttcagatcg tgcgcctcgc ctccacgatc ggcattgcgc tcgtcgtgct
     1861 ggccggggcg tcgtggctgc tgcgcatccg cgagttcacc gagagcgtgt cgctcgtgac
     1921 gaagcggctg cggcgcgccg gccgatgacc cggcggggac ggcgcggccg cctggacggc
     1981 cgttgcgctg ccagcgatgt tgccgagcgc ccgccggttg gcacagactg agagcgatgc
     2041 gcggtcgtta cccccagctg tcggagatca ggtacgcgtt cggcccaggg ccgatgacgc
     2101 ccgcggtcaa agccatcctg atcaccaacg tgatggtgtt cctcgtcact tacttcgcaa
     2161 actcttctcc actggaaggg aatcccgtca ttcggctgct tggcctccag cccgcctcgt
     2221 tgttcggaaa gttcacgatc tggcagccga tcacgtacat gttcgtgcac gggggcgtgt
     2281 cccacatctt cttcaacatg ctgacgctct ggctcatcgg cgtggagctc gagcgcctgt
     2341 gggggacgac ctacttcacg aagttctact tcgtttctgg tctcggagcg ggcttcacgc
     2401 aggtgcttct cggaatcctt cccttcgact tcgccaacca gttctacctg ccgattacca
     2461 tcggagcatc cggtgccatc tacggcctgc tcctcgcctt cgcgctctac tacccgacgc
     2521 gtccgttcct ggtcttcttc atctttccgg tgcaggcgag gtacttcgtg atgatcctcg
     2581 gcgggctctc attgctgttc tcgctgggcg gcggctcggg cgtggcgcac accgcccatc
     2641 tcggggggct cgtgaccggc tacctctatc tgaggcgccc ccgcattacg ccccggatca
     2701 acatcttctc ggagattcaa taccggttcc tgaagtggcg catcaaccgg atgcggcgca
     2761 agttcgatgt ctactccggc ggacgacgcg acgactggga tcggcgggtg cactgaaccg
     2821 aggccccagg gctcaggcct cagggcccag acctcctggc caggcctcag ggcccaggcc
     2881 tcggggcctc aggcctcagg tttccctctc tggttccgag acccagtctt tccggatcgg
     2941 gctgaacatg tcgagttcga acgtgtcttc gagcgcctcg gcctgatgcg gcgtcccgga
     3001 tggcacgtgc agcacctcgc cttcgcggac gatgacttcc tcgccgtcca cgagaaaacg
     3061 gagcgctcct tgcagcacgt acgtcatctg ctcgctctcg tgagagtgga gggggaccag
     3121 ggcgccccgc ttcaggtatg tctgcgcgat catctcccgg tccccggtga caatcttctg
     3181 ggaaagcatt tccgtgacgc gttcgagggc gatctcgtcc caccggtgca atcgcgcggc
     3241 catgtcgggc cccagcatat caaccgaaga ccgtagaccg aagaccgaag accgggtgat
     3301 aatttctaca gatgaagggg tctggcctgg ccacgggcgc tgagtcgcgc gcggccgaac
     3361 accagggacg cagtcgtgcc tacgaagggc tgacgcggaa tgagctggtc gacgcctatc
     3421 gcctgatgct cctgtcgcgg aagatcgacg acagggagat ccagctcaag aaccagacca
     3481 aggcattctt ccagatcagc ggcgccggcc acgaggccgt gctcgtcgcg gccggcaggc
     3541 acctgcgtcc gggcatcgac tgggtgttcc cgtactaccg cgatcgggcg ttgtgcctga
     3601 ccatcggcgt gacgccgctc gagatgttcc tggcctcggt cggctccaca gacgaccccg
     3661 ccaacgcggg ccgccagatg ccctcgcact ggggacaccg ccgctggaac attccttccc
     3721 agagcagctg cgtgggcacg cactgccttc acgccgtcgg cagcgcggag gcggggatgc
     3781 tctacggccg cctgccgcag atcgaagatc gcgaggacta ctactctccc gacgagatcg
     3841 tctacgtctc gatcggcgac ggcgggacca gcgagggcga gttctgggaa tcgctcaaca
     3901 ccgcgtccac caggcacctg ccggtgctgt atctgatcga agacaacggg tacgccatct
     3961 cggtgcccac cgacgtgcag actcccggcg gcgatatcgg tcgcgtgctc gaagggtatc
     4021 ccggcctgcg cacgttccgc tgcgacggca cggactacct gtcgagctac cggacactgc
     4081 gagatgctgt ggaccacgtg cgcgagcgca acggtccggc gctcgtgcac gcgaccgtga
     4141 tccgcccgta ctcgcattcg ctctccgacg acgagaagct gtacaagacg cccggcgagc
     4201 gggaggcgga agcacgccgc gatccgttga cgcggatgcg ccagttcctg ctcgccgagg
     4261 acctggccag cgagcaggac ctcgccgaca tgatggcctc ggtggagaac gaggtgaagg
     4321 cggccgccga agaggcgcag cgcgccgccc agcccgaggc gtcgtcggcg gcgcatttcg
     4381 tcttctcgcc ggacgtcgat ccggcgtcga gccagttctc gaccgaagcg cgtccgcagg
     4441 gggcgccaga caccatggtg tccgccatca acgccacgct gaaggacgag atggcccgca
     4501 acccgcgcat catcgtgttc ggccaggacg tcgcggacgt gagccacagc gaagcgctca
     4561 cgctcgtggc aggcaagggc ggggtgttca aggtcacgca cggactgcag cgcacctacg
     4621 ggagcgaccg ggtcttcaac tcgccgctcg ccgaagccaa catcgtgggc cgcgccgtcg
     4681 gcatggccct tcgggggttg aagccggtcg tcgagatcca gttctttgac tacatctggc
     4741 cggcgttcat gcagattcgc aacgagttgt cgatgatgcg gtaccgctcg ggcaatcact
     4801 ggtcgtgccc ggtcgtgatc cgcgtgccga ttggcggcta cctgcgcggc ggcgcgccgt
     4861 accacagcca gtcgggcgtc tcgatcttcg cgcacacgcc gggactccgc atcgtgcttc
     4921 cctccaacgc gcaggatgcg gcggggctgc tgcgcacggc gatcaggtgc gacgatccgg
     4981 tcctcttctg cgagcacaag cacctctacc ggcagaccta caacaagggc gcctatccgg
     5041 gccccgacta catggtgccg ttcggcaggg ccgccgtacc ccgggaagga cgcgacgtcg
     5101 tggtattcac gtggggcgcg ctcgtgcagc gctcgctcgt cgcggcccag caggccgagc
     5161 agctcggcgt gagcgtggcg gtcgtcgatc tgcggtcgat cgtcccgtac gactgggaga
     5221 cgattgcggc ctacacgaag aagaccagcc gggtcatcgt cgcgcacgag gaccagctga
     5281 ccggcggctt cggcgccgag atcgccgcgc ggatctccga ggagctcttc gagcatctcg
     5341 acgcgcccgt gaagcgcgtc gcggcgctgg attgcccggt cgcctattct ccggtcctcg
     5401 aggaggcgat cctgccggga accgaagacg tgctcgaggc agtccgcgcg atcgtcgctt
     5461 actaggctgg gcctgggccc tgaggcctga ggcctgaggc ctatcgtggt tcagtgactc
     5521 gcatcaacgt catcgcgctg tccctcctgc tcgtcgccct cgccgcgctt ccggacgcag
     5581 cgccggccgt cgacgtgctg cgatcgtccg ggggcctgcc tgcccacatc gtgggcgcct
     5641 acgaggagcc ggtcgatttc cagcaggcaa gcgacggaac ctacttcgtg ttcaacaggc
     5701 gcgggcacac ggtgcacgcc gttgacgcgg cacgccaggc gtcgcggaag gtcgtcgtcc
     5761 tgggaggaga agaggggcgc atcattcagc ccagcggctt cgatgtcact cccgacggcc
     5821 gcatcgtggt cgccgacatg ccgagatcgc agcagcgcgt gcagacgttc gacgcacagg
     5881 gagcgaggct gagcggcttc ttcctgcccg gccggcccgt tgcccgcgtc accatcgaca
     5941 acgtgatgct gaacggcgcg ggatcggtgc agcacacggg gagcacgctg ctcatcagcc
     6001 accctgagag cggggcgctc gtcaccgagt actcgcctgg cgggtacgcg cagcgcagca
     6061 tggggcgcct gcgtcctacc gggttcgagg aggaccgcga gctgcacgtc gcgttgaacg
     6121 cgggcctgcc gctcgtgact ccggacggcg gcttctacta cgtcttcgtg acgggaacgc
     6181 cgatcttccg caagtacgac gccaaggggg tgctccagtt cgaacgccac gtcgaagggc
     6241 gggagctcga cgcctctctg gccgcgcagc ccacgcgctg gccgcgacgc aaggtcgagg
     6301 gcgatgaagt gccgttcgtc gcgccgatta tccgggcggc cgccgtcaac gaacgcggac
     6361 agctctggat ctctctcacc gtcccctaca cctacgtcta cgacgcggac ggagacaaga
     6421 tcaggaccgt ccagttccgc gccgccggcc tcatcagccc aatcagcctg tcgttctcgc
     6481 ccagcggccg tctgctggtc acgcccggct gctacgaatt cgatccgcgg taagccacga
     6541 acagcacgaa gatcgcgaga gcacatcctc gccatgcgac cagcctgaca cctcgaccgt
     6601 cagaccggtt gactgccaac agctgtgagg tgtaggctcc caacagatga cgccagacaa
     6661 acgcgacgtt ctctacggca ccctcggttt gatggtcatc aagaccctgg aggcattggg
     6721 cccgctgcac gggtatcgga tcgcccggcg aatcgaacag atcagcggga atcagctttc
     6781 actcaaccag ggcacgctgt atccggcgct gctcaagctg gaggagatgg ggtggatctc
     6841 gtccaagtgg ggcacgtccg agagcggccg tcgcgtgcgg atctacgcgc tgactcgcgc
     6901 cgggcgcaag cagctccaga acgaggaagc gcagtggcag cgcgcggcga gtatcgttga
     6961 acgtttcctg aagatctcgc agaaccccgc atgacacgcc tgcgcgccgt tctgttccgg
     7021 ctgcgggcgc tttttcgctc ccggcagatg gatcgcgaga tcgacgaaga gatcaccagc
     7081 catctggccg aagcgacaga agagtacgtc cagcaaggcc tttccccgga cgaagcgcgc
     7141 cgcgccgcgc agcgtcgttt cggtggcgtc acgcagacca aggaggtgta tcgggaggtc
     7201 tggtcgatcc ggtggctgga ggatctggcg cttgacgtgc gccatgcgct tcgcaccctc
     7261 cgcaagagtc cggcatttac gacggccgcg gcggccacgc tggcaacggc gattggcacc
     7321 aacaccgcca tgttcagcgt gctgaatgcg gttctgcttc ggccgcttcc gtatcggtcc
     7381 cccgaacagt tggcgatgct gtggaccgag gatccgacgc agaacctccg cgagggccga
     7441 tcggctctgt gggacgtcga gcagtggcgg agtcacagtc agagtgtcgc ggacatggcc
     7501 accttcgatt cggtgtccac ggtactgacc ggcgctgacg gggcggagca gatcgccggc
     7561 gcgagcgtct cccccaacct gctcccactc ctcggtgtcc gccccgtcct ggggcgcagc
     7621 ttttcggctg aagaagccga gcagcggcaa caactggtcc tgatcagcca tcgcttctgg
     7681 caagcgcgat tcggaggctc gcacgatgcg atcggcgcga cgctcgtgct caatggattt
     7741 ccttcccgga tcgtcggcat cctccctgct gatttccaga tcgcgaactt cgatgccgac
     7801 gtgtgggggt cccacacgac ccgccgcagc gtgcgcggcc cacagacgtg gttcgtcatc
     7861 gcgagacttc gcccgggcgc gacgttcgag caggcccagg tcgagatgag cgcagtcgcc
     7921 cgccgcctca acgatccatt gccagcggcc gaaaggaacc ggggcatcag cgtcgtccca
     7981 ctgagtcttt acgtggttgg accgcaatcg cgcctggcgc tgtggctact cggcggcgcg
     8041 gtgttcttcg tgttcctcat cgctgcggcc aatgtcgcag gcctctcgct ggcgcgcggc
     8101 accgctcgca cacgcgagat ggccgttcgc gccgcgcttg gagcgagtgc cggccggatc
     8161 gcgcggcagc tgctcacgga aagcgtcgtg ctcgccatcg ccgggggatt gatgggcacg
     8221 ctgctggccc ttggaggcat tcggctcatc cgtgccttcg gccctggcaa cttgccgcgc
     8281 ctgaatgacg tgagcctcga cctccgcgtt ctcggctggg ccctggcagt ctcgcttctg
     8341 gcgggaatcc tggtggggct ggcgccggcg attacggcca tgcgccgaga cctgcgtccg
     8401 tccggcgagg agagcggcag gagcgtctcg ggcggaacat cgagccgacg aatccgccgc
     8461 ggtctcgtgg tggcggagtt tgcgctggcg atcgtcctgc tcgtcggcgc cggcctgctc
     8521 gtccggagct ggtggtacgt gaacaacatc gatctgggat tcacgcccga gcgggtcctt
     8581 gtgatggggg tctccacccc gacgaccttc aacgaccccg cgcaacgggc ccggctgtac
     8641 caccgcgtcc tcgaacagat ccaggcagtt cctggtgtgg agagcgccgg catcaccggc
     8701 gatctgttcg tttccaaccc cagggaacgg gtcctgacaa tcgaccggga cgacggaacc
     8761 gtgtccgagc gcctgccctt cgcgagagac gaggtcagcc cggatttctt cagaaccctc
     8821 ggaacgccgt tgctgcgcgg ccgcttcttc acgattgggg atcaacccgg gactccgccg
     8881 gtcgccatca tcaacgatgc gatggcccgg cgctcgtggc cggggcagga tccggtgggc
     8941 agaaggttca tgttgggccc gcgggattcc ggcggcccgt ggtacgcggt ggtgggcgtg
     9001 gttgccgata tgcggcggca ggggc
//
LOCUS       DBNGSH010000750         4061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_253046, whole genome shotgun sequence.
ACCESSION   DBNGSH010000750 DBNGSH010000000
VERSION     DBNGSH010000750.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_253046"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="AC16CY_17240"
     CDS             <1..341
                     /locus_tag="AC16CY_17240"
                     /inference="COORDINATES: protein motif:HMM:NF017454.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HIR3355599.1"
                     /translation="GRATINDLKIVKRVDAASTALMAALRTNEPIQKAVLTLRKAGQR
                     PVEYLKVTIENGRVTSLTIDGGDPGGGPDVVENVSFSFNKIEVEYVPQGRDGQPQGGM
                     LFSDQWSDQS"
     gene            347..1183
                     /locus_tag="AC16CY_17245"
     CDS             347..1183
                     /locus_tag="AC16CY_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HIR3355600.1"
                     /translation="MTLAPAAELSLKGGDPAAALARLQEQVRAKPADPKLRVFLFQLL
                     CVLGQWERALTQLDVASGLDPEALAMAQTYGDAVRCEAVREDVFEGKKSPMIFGQPDQ
                     WLALLIESLLESGRGGQARSHDLRARAFDEAPPSEGNIDGRPFAWIADADSRLGPVLE
                     AVINGRYYWVPFSRLIKIELEPPEDLRDLVWMPARLQFENGGESVALIPTRYPGSEGS
                     SDGQITLARKTVWEEIGPEMHRGLGQRLLVTDAEEAPLMEVRTISLSAQEETADRQHD
                     HG"
     gene            1176..1700
                     /gene="tssE"
                     /locus_tag="AC16CY_17250"
     CDS             1176..1700
                     /gene="tssE"
                     /locus_tag="AC16CY_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HIR3355601.1"
                     /translation="MAEQLLPQDRLQPALLDRLTDEEPDKTQEPLEARVVSKSRLRQS
                     VLRDLAWLFNATRLEAVADLTTMPAVRRSVVNFGLPALSGRTASSLDVTDLARAVREA
                     IVTFEPRILPATLQVKTLLDAGALDHHNVIGVEIRGELWAQPVPLEFLVRTEFDLETG
                     NVQVSDLAPSSRVS"
     gene            1700..3574
                     /gene="tssF"
                     /locus_tag="AC16CY_17255"
     CDS             1700..3574
                     /gene="tssF"
                     /locus_tag="AC16CY_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HIR3355602.1"
                     /translation="MDPRLLRYYNLELQHLRETGAEFAEHFPKIAARLGMHGLEVDDP
                     YVERLLEGVAFLAARIQLKLDAEFPRFTQSLLEIVYPHYLAPTPSMLIAQFTPDMDDP
                     GLAGGFKVPRGVTLTGQVLAADAGECEFRTAHDVTLWPVEIVSASYFSFAPDLPLNSL
                     PIAQRIKGGLRIRLKTPQGLGFAKTAIDRLCFHLSGRDDVANKLYELCLASGLGALVL
                     PFARPAPWHELLPAASIRPVGFTDDEALLPVTLRSFQGYRLLQEYFSFPQRFRFFEVS
                     GLSRALRRVGSNQIELVILLGRGDPTLESVVDESNLALHCTPALNLFAKRADRIHVTD
                     SAHEYHVVPDRTKPLDFEVYSVTSVEGHGVGTDSEQRFLPFYTAYSSDEAHQRSAYFT
                     TRREPRLQSLTAKRRGPRSSYIGTEVFLSLVDPSQAPYSGDLRQLSIETLCTNRDLAL
                     QVSKGNSAHDLSLDVAAPVKGTSVISGPSRPYAPLADGAVSWRAISHLSLNYLSLVQA
                     TAEEGAAALRDLLELYAPSADHSARRQIEGIRSVRVGRVVRRLQAPGPLAFGRGLEIA
                     LQVDELAFEGGSAFMLGSVLDRYFARHVSINSFTETVLRSDNREINRWGAQWGARPTL
                     "
     gene            3550..>4061
                     /gene="tssG"
                     /locus_tag="AC16CY_17260"
     CDS             3550..>4061
                     /gene="tssG"
                     /locus_tag="AC16CY_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HIR3355603.1"
                     /translation="MGREADAVAFFAALGEAPYRFDFYQALRRLECLHDGKPRWGEAL
                     RPVDEPVRLGQEPDVSFAPAPLASFGAADDGPPRLQVRLFGLFGPNGPLPLHVTEYAR
                     ERLRLKGDPTLSRFLDVFHHRLLALFYRAWAQAQPHVSHDRPGVDRFMVYVGSFLGMA
                     PNEARDRDATP"
BASE COUNT          628 a         1393 c         1357 g          683 t
ORIGIN      
        1 cgggcagagc caccatcaac gacctgaaga tcgtcaagag ggtggacgcc gcgtccacgg
       61 cgttgatggc ggcgctgcgg acgaacgagc cgatccagaa ggccgtgctg acgctgagaa
      121 aggccgggca gcgtccggtc gagtacctga aggtcacgat cgagaacggc cgcgtaacct
      181 cgctcaccat tgacggcggc gatccgggcg ggggcccgga cgtcgtcgaa aacgtcagct
      241 tctcgttcaa caagatcgag gttgagtacg tgccgcaggg aagggacggc cagccgcagg
      301 gaggcatgtt gttctccgat caatggagcg accagtcgta accgccatga cgctcgcccc
      361 agccgccgaa ctcagcctga agggcggtga ccccgccgcg gcccttgcgc ggctgcagga
      421 gcaggtgcgc gccaagcccg ccgatccgaa actgcgagtc tttctctttc agctgctttg
      481 cgtgctcggc cagtgggagc gggcgctcac gcagctcgac gtggcgtccg gcctcgaccc
      541 cgaggcgctg gcgatggcgc agacctacgg cgacgccgtg cgctgcgaag cggtccgcga
      601 ggacgtgttc gaggggaaga agtcgccgat gatcttcggc cagccggatc agtggctggc
      661 gctgctcatc gagtcgctgc tcgaatcggg ccgcggaggc caggcgcggt cgcacgacct
      721 gcgtgccagg gcgttcgacg aggcgcctcc gtcggaaggg aacatcgacg gacggccctt
      781 tgcctggatc gccgacgccg actcacgcct cgggccggtg ctcgaggcgg tgatcaacgg
      841 caggtattac tgggtgcctt tctcccgcct gatcaagatc gagctcgagc cgccggagga
      901 ccttcgcgac ctcgtctgga tgcctgcccg cctgcagttc gaaaacgggg gcgagtcggt
      961 ggcgctcatt ccgacgcgat acccgggctc cgaggggtcg agcgacgggc agatcaccct
     1021 cgcgcgaaag accgtatggg aggagatcgg tccggagatg caccgcggcc tcggtcagcg
     1081 cctcctcgtg acggatgccg aggaagcgcc cctcatggag gtccggacga tctcgctctc
     1141 ggctcaggag gagaccgcgg atcgccagca cgatcatggc tgagcagctc cttccccagg
     1201 atcggctgca gccggcgctg ctcgatcgtc tcaccgacga ggaaccggac aagacgcagg
     1261 agcctctaga ggcgcgcgtc gtgtcgaaga gccgcctgcg ccagtcggtg ctgcgcgacc
     1321 tggcctggct gttcaacgcc acgaggctcg aagcggtcgc cgatctgacg acgatgccgg
     1381 cggtgcgccg ctcggtggtg aacttcgggc tgccggcgct gtcgggccgt actgcatcgt
     1441 cgctcgacgt gaccgatctg gcccgtgccg tccgcgaagc catcgtaacg ttcgagccac
     1501 gaatcctgcc ggcgacgctg caggtcaaaa cgctcctcga tgccggcgcg ctcgatcacc
     1561 acaacgtcat cggcgtcgaa atccgcggcg agctgtgggc gcagccggtg ccgctcgagt
     1621 tcctcgtccg caccgagttc gacctggaga caggcaacgt ccaggtctcg gatctcgccc
     1681 cgtcgtcgcg ggtgtcgtaa tggacccgcg tctgcttcgg tactacaacc tcgagctgca
     1741 gcacctgcgc gagacgggcg ccgagttcgc cgagcacttt ccaaagatcg ccgcccggct
     1801 cgggatgcac gggctcgagg tggacgatcc gtacgtggag cggctgctcg agggcgtggc
     1861 gttcctggcc gccaggattc agctcaagct cgacgccgaa ttccctcggt tcacgcagtc
     1921 gctgctcgag atcgtctatc cccattacct cgcgccgacg ccctcgatgc tcatcgccca
     1981 gttcacgccc gacatggacg atccgggcct cgcgggcgga ttcaaggtgc ctcggggagt
     2041 caccctgacc ggccaggtgc tcgccgccga tgcgggggag tgcgagtttc gcaccgctca
     2101 cgacgtgacg ctctggccgg tggagatcgt gtcggccagc tatttctcgt tcgcgccgga
     2161 cctgccgctc aactcgctgc cgatcgcgca gcgcatcaag ggcggtctcc gaatccgcct
     2221 caagacaccc caggggctcg gattcgcgaa gaccgcaatc gaccggctct gcttccatct
     2281 gtcgggccgc gatgacgtcg cgaacaagct gtacgagctg tgtcttgcgt ccggcctcgg
     2341 cgcgctggtg ctgcctttcg cgcgccccgc tccatggcac gagcttctcc cggccgcctc
     2401 catccggccg gtgggtttca ccgacgatga ggcgctcctg cctgtgacgc tgcgttcgtt
     2461 tcaagggtac cggctgctgc aggagtattt ttcgttcccg cagcgctttc ggttcttcga
     2521 agtgtccggc ctctctcgtg cgctgaggcg cgtcggctcc aaccagatcg agctcgtgat
     2581 tctgctcggc cgcggcgacc cgacgctcga gagcgtcgta gacgagtcga acctcgccct
     2641 tcactgcacg ccggcgctca acctgttcgc caagcgcgcc gatcgcatcc acgtcaccga
     2701 cagcgcgcac gagtaccacg tcgtgcccga ccggaccaag ccgctcgatt tcgaggtcta
     2761 ctccgtcacg agcgtggagg gccacggcgt cggcaccgac agcgagcagc gcttcctgcc
     2821 gttctacacg gcgtacagct cggacgaggc ccatcagcgc tcggcctact tcacgacgcg
     2881 ccgcgagccg cgactccagt cgctcaccgc gaagcgccgc gggccccgat cgagctacat
     2941 cgggaccgaa gtcttcctct cgctcgtcga tccgtcgcag gccccgtaca gcggcgacct
     3001 gcgtcagctc tcgatcgaaa cgctctgcac caaccgcgat ctggcccttc aggtgtctaa
     3061 agggaacagc gcccatgacc tctcgctcga cgtggcggcg cccgtgaagg gcacgagcgt
     3121 gatcagcgga cccagccgtc cgtacgcgcc gctggcggac ggcgccgtgt cgtggcgcgc
     3181 catcagccat ctgtcgctca actatctgtc gctcgtgcag gcgacggcgg aggaaggagc
     3241 tgccgcgctg cgcgatctgc tggagctgta cgcgccgagc gcggaccaca gcgccaggcg
     3301 ccagatcgaa gggattcgat ccgtgcgtgt ggggcgcgtg gtgcgccggc tgcaggcgcc
     3361 cggcccgctc gccttcggcc gcgggctcga aatcgcgctg caggtggacg agctggcgtt
     3421 cgagggcgga agcgcgttca tgctggggtc tgtgctcgat cgatatttcg cacgccacgt
     3481 ctccatcaac tcgttcacgg aaacggtgct gcgatcggac aaccgcgaga tcaaccgatg
     3541 gggggcgcaa tggggcgcga ggccgacgct gtagcgttct tcgcggccct gggcgaagcg
     3601 ccgtatcgct tcgacttcta ccaggcgctt cggcgcctgg agtgcctgca cgacgggaag
     3661 ccgcgctggg gggaggcgct tcgtcccgtg gacgagcccg tgcggctcgg ccaggaaccg
     3721 gacgtgtcat tcgcgccggc gccgctggcc tcgttcgggg cggccgatga tgggccgcct
     3781 cgcctgcagg tgaggctgtt cggtctcttc ggccctaacg gcccgctgcc gctccacgtc
     3841 acggaatacg cgcgcgagcg cttgcgcctc aagggcgatc cgaccctcag ccgttttctc
     3901 gacgtcttcc atcatcgcct gctcgcgctg ttctatcgcg cgtgggccca ggcgcagccc
     3961 cacgtcagcc acgatcggcc gggcgtcgat cgcttcatgg tgtacgtcgg gtccttcctc
     4021 gggatggcgc cgaacgaggc tcgcgaccgc gatgccacgc c
//
LOCUS       DBNGSH010000751         5284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1102514, whole genome shotgun sequence.
ACCESSION   DBNGSH010000751 DBNGSH010000000
VERSION     DBNGSH010000751.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1102514"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="AC16CY_17265"
     CDS             <1..109
                     /locus_tag="AC16CY_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HIR3355604.1"
                     /translation="DLWEHAYYLKYQNRRADYVRAWWSVVNWTEVNKAY"
     gene            280..1362
                     /locus_tag="AC16CY_17270"
     CDS             280..1362
                     /locus_tag="AC16CY_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3355605.1"
                     /translation="MSNVHCGVMAERAAVDIPPSLGPDTLPRRPDPVPYHGIAKHAFG
                     QLVGTSALMREVYDHISRVAPTSATVLIVGESGTGKELVARTLHDLSRRRLGPYIALN
                     CSALSPMLIESELFGHERGSFTGADRRHHGVFERAMRGTLFLDEVTEMPAELQVKLLR
                     VLETGTFTRTGGETPIAVDVRFLAATNRRPEEAIKQGKLREDLYYRLKVFQLSLRRLR
                     DRPEDVPLLAEHFLTQTAALEGQTKQFTSEALAMLTAYVWPGNVRELKNAVYSAYILA
                     GSEITVDCLPVEIAMPEPTPAPGCNIPVNIGMTAAEAERRLILATLSHFTGSKSKTAE
                     TLGISLKTLYNRLQEYRVQEYGQPTA"
     gene            1635..2411
                     /locus_tag="AC16CY_17275"
     CDS             1635..2411
                     /locus_tag="AC16CY_17275"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3355606.1"
                     /translation="MSARPAGDLSSRSFHHEVLASLAHADVPFLVGGSVAMARHAGVK
                     RGIEDLHLFLRRDDWPGAEAALNHAGIHTHVAFSHWLAKAHEGDRTVDIVYNGGNGLT
                     PVDARWFDRAFFDEVVGLKVRLCPPEELIWSKAFVMEREQFHGADVLHLIRARHDGLD
                     WDRLLELFERYPRVLLAHLVLFGFVYPGGSDTVPPWILAHLWSRIGEERRDDFSLLCR
                     GTVLSRGQYLIDVEHWGYTDARVFPFGTMTRQEIESDRRD"
     gene            complement(2424..4760)
                     /locus_tag="AC16CY_17280"
     CDS             complement(2424..4760)
                     /locus_tag="AC16CY_17280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3355607.1"
                     /translation="MKRITLLTCGRIIAVTSVLALLLAAAEVHRVYFDRRNMPDLGPF
                     VRFEFPTTGHIYDAGDRPLIELARERRERSQYRDIPPIVRDAILAAEDKRFFSHNGID
                     YLSLPRVLGKVRLGMLAGRLARGGRHDERRGAALFPQGGSTITQQLVRGHFLRGVTAL
                     ENSHELRPTGLGARALSSVIGARSVNMLARKREEMRLSFWLEREMRDQFGSKRRAKEE
                     ILARYATFVYMGNGQYGFARAAQYYFGRPLATFTADDADKAALLAGIAKSPREYAPSA
                     RNGDLVVRRRNQTLALMRANGFLSPEAAAHAIGRALDIVRDDTRQALQAPAVVEHVLE
                     EIRFRHEDLSVEDLLQGRIQVYATADARVQDIVNDALEHGLVRYEERHPRARGMIQGS
                     VVVLHNRDGRILAETGGRRVFNGQSASYSDYNRVTKSLRQPGSAMKPLVYLAAFRSGD
                     FTLETLVPDEPISVPNGPSRPAKWISNYDGRFKGLIPVRQALAESRNAVAVWITEQIG
                     IDRVLRTSRSLGIRTPLQPFATTALGASETSLLELANAYRTMASGIVAEPYVIRAVVR
                     DSGEIVTATRLRALPTSIGGTALSLIQEGLRGVVRLPTGTAHTLASRGFPIAVMGKTG
                     TTSDFKDAIFVGSTYGPDGITAAVRIGFDDNRSLGSKETGGRLALPVFQELMLRIYRD
                     RVVGVAPSFPAWMEASITASLVPPAPDEADLARTGIPSVFRAGHRPASAAETVWLLTQ
                     GLRIRTPSAALPFPTRLPGTSVFANQASGSPRRDAVGP"
     gene            complement(4788..4940)
                     /locus_tag="AC16CY_17285"
     CDS             complement(4788..4940)
                     /locus_tag="AC16CY_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HIR3355608.1"
                     /translation="MLTILLVVLVLMLLGAMPSWRHSRGWGYYPSGGLGLIVLVLVVL
                     MLSGRL"
     gene            5119..>5284
                     /locus_tag="AC16CY_17290"
     CDS             5119..>5284
                     /locus_tag="AC16CY_17290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3355609.1"
                     /translation="MSVAPHPLLAAIGEGRTLLAYPKGRAIYAQGDEANAVFYVQKGR
                     VRLTVVSKAGK"
BASE COUNT          935 a         1733 c         1715 g          901 t
ORIGIN      
        1 cgacctgtgg gaacacgcct attacctgaa gtaccagaac cgacgggccg actacgtcag
       61 ggcctggtgg agtgtcgtga actggaccga ggtcaacaag gcgtactgaa gcgctccgcc
      121 gcccgccagc cgtgccgtcc ccgatgcgtg tctttgtgaa cacgtgtctt tttggactgc
      181 gctcacattc tgcatcggtc atatttacct cgcactgaat ctggagtctg aaacccgagc
      241 gttgaatcgg cgatcggact ccatcgcgag gatgaaccag tgagcaacgt tcattgcggc
      301 gtgatggcgg agcgggcggc ggtcgacatt ccaccatccc tgggacctga caccctgccg
      361 cgccgaccgg atcccgtgcc gtatcacggg atcgcgaagc acgcgttcgg acagctggtc
      421 ggtacctctg cgttgatgag agaggtctac gatcacatct cgcgggtggc gccgaccagc
      481 gcgaccgtcc tcatcgtggg cgagagtggc accggcaagg aactggtcgc gcggacgctt
      541 cacgatctga gccgccggcg gctcgggccg tatatcgcgc tcaattgcag cgcgctctcc
      601 cccatgctca tcgagagcga gctcttcggt cacgagcgtg gcagctttac cggagccgat
      661 cggcgacacc acggcgtctt cgagcgagcg atgcgcggga ccctcttcct cgatgaggtg
      721 acggaaatgc ccgccgagct ccaggtcaag ctcctccggg tgctcgaaac gggcacgttc
      781 acgcggaccg gtggtgaaac gccgatcgcg gtggacgttc gcttcctcgc cgcgaccaat
      841 cgacgtcccg aggaggccat caagcaaggc aagcttcgcg aggacttgta ctatcggctc
      901 aaggtgttcc agctctcgct gcggcgcctc cgcgaccgcc ctgaggacgt gccgctgctc
      961 gccgaacact tcttgacgca gacggcagcg ctcgaaggcc agacgaagca gttcacgtcg
     1021 gaggcgctcg ccatgctcac cgcatacgtg tggcccggca acgtccgcga actcaagaac
     1081 gcggtgtaca gcgcctatat tctggctggc tccgagatca ccgtcgactg tctgcccgtc
     1141 gagatcgcca tgccagagcc aacccccgcg cccgggtgca acatcccggt gaacatcggc
     1201 atgaccgcgg cggaggctga gcgacgtctg atcctcgcga cgctctcgca cttcactggc
     1261 agcaagagca agacggccga gacgctgggg atcagtctca agacgctcta caaccgcctt
     1321 caggagtacc gcgtccagga gtacggtcag cctacggcgt agaccgagac tcgacgggat
     1381 cgtcggacga aagaacgacc aaggaccgga gttcgaggcg gcttcgggcc agtggagcca
     1441 tcatcgcctc ctccccgcag tgccgtcccg tcttgcccca ggcgggacgg ccccaccttg
     1501 cccgaccagc aaatcttacg aacatgtgta aagcgttcgt gtccgggaac gaaggtgacc
     1561 tcgtccctcg gcccagttgc attcggatcc gtcggtatac gtccccggca cactccttgc
     1621 tcccactcga cgccgtgagc gcgcggcccg caggcgacct gtcgtctcga agcttccacc
     1681 acgaggtgct tgcgagcctg gcgcacgcag acgtgccctt tctggtcggc ggcagcgtcg
     1741 cgatggcccg ccacgcgggg gtgaagcgtg ggatcgagga tctccacctc ttcctgcgcc
     1801 gcgacgactg gccgggggca gaagcggcgc tgaaccacgc cgggattcac acacacgtcg
     1861 ccttctcgca ctggctcgcc aaggcccacg aaggcgatcg gaccgtcgac atcgtctaca
     1921 acggtggcaa cggcctcaca cccgtggatg cgcgctggtt cgatcgtgcc ttcttcgatg
     1981 aagtggtcgg tctgaaggtt cggttgtgtc cgcctgagga attgatctgg tcgaaggcat
     2041 tcgtgatgga gcgggaacag tttcacggcg ccgatgtcct tcacctgatc cgggcccgcc
     2101 atgacggcct cgactgggac cgcctcctcg agctcttcga acgctatccg agggtgttgc
     2161 tggctcacct cgtcctgttt ggcttcgtgt acccgggcgg gtccgacacg gtgccgccgt
     2221 ggatcctcgc gcatctgtgg agccggatcg gagaagagcg acgggacgat ttcagccttc
     2281 tctgccgtgg cacggtactc tcacgtggcc aatacctcat cgacgtcgag cactggggct
     2341 acaccgatgc gcgcgtgttc ccgttcggga ccatgacgcg gcaggagatc gagtcggacc
     2401 gacgcgattg atccggccca agactacggt ccgacggcgt cacgccgggg gctgccgctc
     2461 gcctgattcg cgaacacgga cgtgccgggc agccgggtcg gaaacggcag ggcggcggac
     2521 ggcgttctga ttctcagtcc ctgtgtcaac agccagaccg tttcggcggc tgatgcaggc
     2581 cgatgccctg cgcgaaacac ggacgggatg cccgtgcggg cgagatcggc ttcatcgggc
     2641 gcaggtggca cgagagaagc ggtgatgctg gcttccatcc atgctggaaa cgacggtgca
     2701 accccgacga cccggtcccg gtagatacgg agcatcaact cctgaaagac cggcagcgcc
     2761 aacctccccc cggtttcttt cgatccaaga gaacggttgt cgtcgaagcc aatcctgacc
     2821 gcagccgtga tgccgtcggg gccgtaggtc gaacccacga agatggcgtc cttgaagtca
     2881 ctcgtcgtgc cggtcttgcc catgacggcg atcgggaacc cgcgagaggc cagcgtatgc
     2941 gccgtgccgg tgggcaggcg cacgacaccg cgcaagcctt cctggatgag cgaaagcgct
     3001 gtgccgccga tagaggtcgg caacgcgcgg aggcgggtgg cagtcacgat ctcgccagaa
     3061 tcccgcacca cggcacggat cacgtacggc tcggccacaa tccccgaggc catggtgcgg
     3121 tacgcattcg ccagttcgag aagactcgtc tctgatgcgc cgagcgccgt cgtggcgaag
     3181 ggttgcagtg gcgtccggat gccgaggctc cgtgaggtcc gcagcacccg gtcgattccg
     3241 atctgctcgg tgatccacac ggccacggca ttcctggatt cggcgagcgc ctgacggacg
     3301 ggaatcaggc ccttgaaccg gccgtcgtag ttcgagatcc acttggctgg acgcgagggt
     3361 ccgttcggca cgctgatcgg ttcatcgggc accagcgtct cgagggtgaa gtctccggag
     3421 cgaaaggccg ccagatacac cagcggcttc attgccgatc cgggctggcg cagggacttg
     3481 gtcacacggt tgtagtcgct gtaggacgcg gattgtccgt tgaagacgcg ccggccgccc
     3541 gtctcggcca ggatccggcc atcgcggttg tgcagcacca cgaccgagcc ctggatcatc
     3601 ccgcgcgccc gcggatgccg ctcttcgtac cggacgaggc catgctccag ggcgtcgttg
     3661 acgatgtcct gtacgcgagc atccgctgtc gcgtagacct ggattcgacc ctgcaggagg
     3721 tcttccacgc tgaggtcttc gtggcgaaat ctgatctcct cgaggacatg ctccacgacg
     3781 gccggcgcct ggagcgcctg gcgcgtgtca tcgcgcacga tatcgagcgc gcgcccgatg
     3841 gcgtgcgcag cggcctcggg cgacaggaac ccattcgccc gcatcagcgc cagcgtctgg
     3901 ttccgccgcc tcacgacgag gtccccgttc ctggcgctcg gcgcgtactc gcgtggcgac
     3961 ttggcaatac cggcaagcag ggccgccttg tcggcatcat cggcggtgaa ggtcgccagg
     4021 ggccgcccga agtagtactg cgccgccctc gcgaacccgt actggccgtt gcccatgtac
     4081 acgaacgtcg cgtatcgggc gaggatctct tccttcgcac ggcgcttcga tccgaactgg
     4141 tcccgcatct cgcgctccag ccagaacgac agccgcatct cttcgcgctt ccgggccagc
     4201 atgttgacgc ttcgggcgcc aatgaccgac gacaacgccc gtgcaccaag cccggttggg
     4261 cgcagctcgt ggctgttctc cagcgccgtg acgccgcgga ggaaatggcc ccgcacgagc
     4321 tgttgcgtga tggtggatcc tccctgcggg aacagcgccg cgccgcgccg ttcgtcgtgg
     4381 cggccgcccc tcgccagacg cccagccagc atgccgagcc tgaccttccc gagcacgcgg
     4441 ggcaggctca gatagtcgat cccgttgtgg gaaaagaagc gcttgtcctc ggcggccagg
     4501 atcgcgtcac ggacgatcgg tggaatgtcc cggtactgcg agcgctcgcg gcgctcccgc
     4561 gccagctcga tgagcggcct gtcgcccgca tcgtagatgt ggccagtcgt cgggaactcg
     4621 aaacggacga agggaccgag gtccggcatg ttgcggcggt cgaagtacac gcgatgcacc
     4681 tcggcagccg ccagcaggag agccagcacc gatgtcaccg cgatgatgcg gccacaggtg
     4741 aggagggtga ttctcttcac gtgcatcctc gcggcggacg accagcgtca gagccgaccc
     4801 gacagcatca ggacgaccag tacgagcacg atgagaccga ggcccccgct ggggtagtaa
     4861 ccccatcctc ggctgtgtcg ccaggacggc atcgcgccca gcaacatcag cacgagcacg
     4921 accaggagta tggtgagcat cgcttctccc ttgatgaaat gaacgttggg actccacgtt
     4981 cagagtagcg actgaggagc gctctgtctg tgcacaactg cacgtgggcg agtgacgtcg
     5041 ggtgcgacgg ccaccggcga gggctgtgcc gcattgcaca gcccagcggc gattgttggg
     5101 gcaagatgaa gagtcatcat gagcgttgcc ccacacccct tgctcgcggc catcggagaa
     5161 ggccggacgc tcctggcgta tccgaagggc cgggcgattt acgcacaggg cgatgaggcg
     5221 aacgcggtct tctacgtgca gaagggcaga gtgaggctca ccgtcgtctc gaaggccggc
     5281 aagg
//
LOCUS       DBNGSH010000752         6553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2460268, whole genome shotgun sequence.
ACCESSION   DBNGSH010000752 DBNGSH010000000
VERSION     DBNGSH010000752.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2460268"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2982)
                     /locus_tag="AC16CY_17295"
     CDS             complement(<1..2982)
                     /locus_tag="AC16CY_17295"
                     /inference="COORDINATES: protein motif:HMM:NF021578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HIR3355610.1"
                     /translation="MPHGSGADDSDLHAPSLAHVVDQLAHLLPAQGPISIFIHHNTLH
                     AFEDLPFETAVERAAGRFGCQPFLSEERYRAKVAAGRILARDVESLLADDLGQGGAED
                     VAGVGTRLDLWRRIVLHGIPAATGRALSWILEETAALSRFRRDLPRDARSAPSAVTDL
                     DEDRTIERTAVRHLWEACLGAVGRAGPAHAIDEVPLVRHRDLLLAAAGIDTDQWIHPP
                     LIRFIAGYLDQGLAYWVMPARDRGVHGCFLDLYGTSLAAQCGPWARALPALIADDRRC
                     ERDELTSLEHSLAEIGVSSDEWTDYLTSTLLALRGWAGMVRQIEERPDRVPARELPAT
                     LAGYLTVRLLFERAALVYAARQIGFDGPLPLLRSFLRARVPRSPVPTDQERAWPLFHV
                     AQLSGLDASIVLQWTAHDVEEIESELRVLDGVRQRQILHRAYERALRYRLYDALVRHP
                     PRQPPASPAFQAVFCIDEREESFRRHLEEIDPAVETFGTPGFFRVAMYHQSASDAHPR
                     PLCPVVIRPEHYVPEIEQDADAFVRRSLRVQRRAAGFLGYNVHLGSRTLVRGALLTTV
                     FGGLMLVPLILRVVFPWLASRWGRVGDTAPARTRLRLDRDEMRPPVGKHVGFTVTEMT
                     RIVHDVLEEIGIAGRLSPLVLVVGHGSISLNNPHESAHDCGACGGGRGGPNARAFAQM
                     ANDPRVRRQLASDGLAIDSSTWFVGAERNTCDNTVTFFDEDLVPAHLQPLFARARETF
                     EATRRQEAHERCRRFDAVPSWYPPLAALAHVEGRAADLAQPRPEYGHATNAYCVIGRR
                     ARTSGLFLDRRAFLVSYDPTFDEDGAILSRVMAAVVPVVAGISLEYYFSYVDPAGYGC
                     GTKLPHNVTSLLGVMDGTQSDLRTGLPWQMVEIHEPTRLAIVVEASRDRLQQVVSANP
                     SIERLVRNRWIWLACLDTHSASLWELRSGGFVVHRPGHSLPIVLGDSATWYHGKRGFL
                     PPVTIAHQASPPPIGRAPDREA"
     gene            complement(3007..3960)
                     /locus_tag="AC16CY_17300"
     CDS             complement(3007..3960)
                     /locus_tag="AC16CY_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3355611.1"
                     /translation="MRLSPWARPSRASGRAWRSRRPLLTLALVASCSACATLPAVTAP
                     SPADVARLPRLGSSLPHADGSVRFLVIGDSGTGGREQYDVATVIARAHATFPFTFALM
                     LGDNLYGLEGAGARVNKFEKPYKPLLDAGVTFHAALGNHDNPNQRLYPPFGMNGKRYY
                     SFRRASVEFFVLDSTGMTPEQVSWLALALHGSDAPWKIAYFHHPLYSSGEKHGSEEAL
                     RAIVEPLFLEHGVDVVFAGHEHFYERLKPQQGITHFIQGGAAKLRKGNILPNSPLTAR
                     GFDTDRSFTLVEVAGDEMFFETISRVGDVIDSGVVRRREAP"
     gene            complement(3905..4906)
                     /locus_tag="AC16CY_17305"
     CDS             complement(3905..4906)
                     /locus_tag="AC16CY_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3355612.1"
                     /translation="MSSTGTGVRTRDVFQAWGRILAGHRPNLSIEITKECPLRCPGCY
                     AYGDEHLGGGVTLRGLSDYTGEQLVSRFMALIDRHGPVHVSIVGGEPLVRFRELNTIL
                     PELARRGVHTQVVTSAVRPIPREWIGLPHLQVCVSIDGLPAEHDVRRAPATYERILKH
                     IDGHQITVHCTVTRQQARRDGYVEEFVSYWQANPNTRLIWISLYTPQIGELSAERLTK
                     ADRERVIAEIGRIRTRYPKLQMRDGMLKALAAPPASPDQCLFAQSTECFSSDFEHRIT
                     PCQFGGNPDCQNCGCLASAGLESMAQHRLPGGISIGRIFYASLAVGQTVARVRQGMA"
     gene            4939..5355
                     /locus_tag="AC16CY_17310"
     CDS             4939..5355
                     /locus_tag="AC16CY_17310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HIR3355613.1"
                     /translation="MIGEPQLPRIARRKSKLHGWGVFALEPINKNRRIVTYNGELIDH
                     KESFRRETIYLERGEIWCFTVNRRWVRDAMVGGNVARFINHACRPNCYSQIIGREIWI
                     RAGRRIEPGEELTYDYCTDGEKVIQCRCRPGCKRRL"
     gene            5352..>6553
                     /locus_tag="AC16CY_17315"
     CDS             5352..>6553
                     /locus_tag="AC16CY_17315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR3355614.1"
                     /translation="MKSLRGLCLVALVLSAAYLAGPEFIASAPGQTDLTDADLVNASG
                     GRTVAIGAVSGDGQVSRIPLELYVARVLAGEGEPNAADAARQALAMAIRTYTIVQMGR
                     HRREGFDLCDTVHCQVLRAANEGSRRAALSTAGRVLTIDGAPAEVYYSASCGGRTEGA
                     AQVWPGATFPYMQVVRDDDVHAEDVPWTLEFTLAELRETLARAGFGGGALRDVEIDGH
                     TPSGRVDRLRLPGLRPDAIAATAFRDALGTTRFRSTAFSLARDGNIVRFTGRGYGHGV
                     GMCVIGAGRRARRGESAEAILAQYYPNLTISSLGHATAPAPPPPPPADRARVKRITAV
                     VPRGSSVGADQLEALAGRAYESLSRALGTTAASVTVELHETLDGFRLEARQPWWVSAA
                     VTGTTIDLA"
BASE COUNT         1119 a         2146 c         2268 g         1020 t
ORIGIN      
        1 ggcttcccgg tcaggagctc ggccgatggg aggcggcgat gcctggtgcg cgatcgtcac
       61 cgggggcagg aatccgcgct tgccgtggta ccaggtggcc gagtccccga ggacgattgg
      121 aagcgaatgc cccggcctgt gtacgacgaa cccgccggac ctgagctccc acaacgacgc
      181 ggagtgcgtg tcgagacagg caagccagat ccagcggttt cgcacgaggc gctcgatcga
      241 cggattggcc gagacgacct gctgcaggcg gtcgcggctg gcttccacga cgatggccag
      301 gcgcgtgggc tcgtgaatct cgaccatctg ccacggcaat cccgtccgca gatcgctttg
      361 tgtgccatcc attacgccaa gcagcgatgt cacgttgtgt ggcagcttgg ttccgcatcc
      421 gtagccggcc ggatcgacgt agctgaagta gtactcgaga ctgatgccgg ccacgaccgg
      481 caccacggcg gccatcaccc gcgacaggat cgccccatcc tcgtcgaagg tcggatcgta
      541 ggagaccaga aaggcgcgac ggtccaaaaa cagcccggac gtgcgcgcac gacggccgat
      601 gacgcagtac gcgttggtgg cgtggccgta ctccgggcgc ggttgcgcca gatccgcggc
      661 ccgtccctcg acgtgagcca acgcggccag cggcgggtac cagctcggca ccgcgtcgaa
      721 acgccggcac cgctcgtgcg cctcctgtcg tcgcgttgcc tcgaacgtct cgcgggctcg
      781 agcgaacagc ggttgcaggt gggcaggaac gagatcctcg tcgaagaagg tgacggtgtt
      841 gtcgcaggtg ttgcgttcgg ctccaacgaa ccacgtggac gagtcgatgg cgagcccatc
      901 ggaggcgagc tggcgccgca cccgtggatc gttggccatc tgcgcgaagg cccgggcatt
      961 gggccccccg cgcccgcctc cgcaggcgcc gcaatcgtgg gccgactcgt gcgggttgtt
     1021 gaggctgatc gatccgtgtc cgacgacgag caccaggggc gacaggcggc cggcgatccc
     1081 gatctcttcg aggacgtcgt gaacgatgcg cgtcatttcg gtgaccgtga acccgacgtg
     1141 tttcccgacg ggtgggcgca tctcgtctcg atccaggcga aggcgtgtcc tggcaggcgc
     1201 cgtgtcaccg actcgacccc aacgggatgc cagccacggg aagaccacac gaaggatcaa
     1261 ggggacgagc atcaacccgc cgaacaccgt cgtcaggagc gcgccgcgga ccagcgtccg
     1321 gctccccaga tgcacgttgt agccgaggaa gcctgccgcg cgccgctgga cgcgcaggga
     1381 tcgccgcacg aaggcatcgg cgtcctgctc gatttccggc acatagtgct ctggacgaat
     1441 gacgacaggg cagagcggtc gtggatgggc gtcgctcgcg ctctgatggt acatggccac
     1501 gcggaagaac ccgggggtcc cgaacgtttc gaccgccggg tcgatctcct cgagatgacg
     1561 ccggaacgac tcctctcgct cgtcgatgca gaacaccgcc tggaacgcgg gcgaggccgg
     1621 cggttggcgc ggcgggtggc ggacgagggc gtcatacagg cgatagcgca gcgcgcgttc
     1681 ataggctcga tgcagaatct ggcgctgtcg tacgccgtcg agcacccgca gttcggactc
     1741 gatctcttcc acgtcgtgcg ccgtccactg caagacgatc gaggcgtcga ggccgctgag
     1801 ctgcgccaca tgaaacagcg gccacgcccg ctcctgatcc gtcggcacgg gagagcgcgg
     1861 gacgcgcgct cgcaggaacg accggagtaa cgggagcggt ccgtcgaagc cgatctgcct
     1921 ggctgcgtac acgagcgccg cgcgctcgaa caagagacgc accgtcaggt acccggcaag
     1981 cgtggctgga agctctcgtg ccggcacgcg atcggggcgc tcctcgatct ggcgcaccat
     2041 cccggcccag cctcgcagcg cgaggagggt ggaggtcagg taatcggtcc attcgtcgga
     2101 tgagacgcca atctctgcga gagagtgttc gagcgaggtc agctcgtccc gttcacatcg
     2161 ccgatcgtcc gcgatcaagg cgggaagggc tcgggcccat ggaccacact gggcggcaag
     2221 cgaggtccca tacagatcga ggaagcagcc gtgaacaccg cggtctcgtg cgggcatgac
     2281 ccagtaggcg aggccctggt cgaggtaacc ggcgatgaag cggatcaaag gaggatggat
     2341 ccactggtcg gtgtcgatgc ccgccgctgc cagcagcaga tcccggtgcc gcacgagtgg
     2401 tacctcgtcg atagcgtgcg cggggcccgc gcgtccgacc gctccgagac atgcctccca
     2461 caagtgccgt acggccgtcc gttcgatggt cctgtcctcg tcgagatcgg tcacagccga
     2521 aggggccgat cgagcgtcgc gcgggagatc cctgcgaaaa cgcgatagcg cggcggtttc
     2581 ttcgaggatc cacgacagtg cccgcccggt ggcggccgga atgccatgca gcacgattcg
     2641 tcgccacagg tcgagacgcg ttcccacgcc ggccacatcc tcggcgccgc cctggcccaa
     2701 gtcgtcggcc aggagtgatt cgacgtctct ggcaaggatg cgccccgcgg cgaccttcgc
     2761 gcggtagcgc tcttcagaga ggaacggttg gcacccgaac cgtccggccg cgcgctcgac
     2821 ggcagtttcg aagggcagat cctcgaacgc gtgcagcgtg ttgtgatgga tgaagatgct
     2881 gatcggaccc tgcgcgggca gcaggtgcgc caactggtcg acgacgtgcg caaggctcgg
     2941 agcgtgcagg tcggaatcgt cagcgccgga accgtggggc atcgagcgta cgcaagtata
     3001 ccggggttac ggagcctccc gccgccggac gacgcccgaa tcgatcacgt cgcccacgcg
     3061 cgagatggtt tcgaagaaca tctcgtcgcc cgcgacctcg accagcgtga aggaccggtc
     3121 ggtatcgaag ccgcgcgccg tcaacggcga gttcgggagg atgttgccct tgcgcagctt
     3181 ggccgcgcct ccctggatga aatgggtaat tccctgctgt ggcttcaggc gctcgtagaa
     3241 atgctcgtgg cccgcgaaca ccacgtccac gccgtgttcg aggaacagcg gctcgacgat
     3301 cgcgcgaagc gcttcttccg atccgtgctt ttctcccgat gagtacagcg ggtgatgaaa
     3361 gtacgcgatc ttccacggcg cgtccgatcc gtgaagcgcc agcgccagcc aactgacctg
     3421 ttccggcgtc atgccggtgc tgtcgagcac gaagaactcc acgctcgccc gcctgaacga
     3481 gtagtagcgc ttgccgttca tcccgaacgg cggatagagg cgctggttcg ggttgtcgtg
     3541 gttgccgagc gcggcatgga aggtgacccc ggcgtcgagc agcggcttgt agggcttctc
     3601 gaacttgttc acgcgggcgc cggcaccctc gaggccatac aggttgtcgc ccagcatcag
     3661 ggcaaacgtg aacgggaagg ttgcgtgcgc gcgggcgatg accgtggcga cgtcgtactg
     3721 ctcgcgcccg cccgtgcccg aatcgccgat gacgaggaac ctgactgaac catcggcgtg
     3781 cggcaggctg ctgccaaggc gcgggaggcg ggcgacgtcg gccggcgatg gagccgtgac
     3841 ggcgggcagc gtcgcgcacg cgctgcacga cgcgacgagg gccagggtca gtagtggccg
     3901 gcggctacgc catgccctgc cggacgcgcg cgacggtctg gcccacggcg agagacgcat
     3961 agaagattct cccgatggaa atcccgccgg gcagacgatg ctgcgccatc gattcgagcc
     4021 cggcagaggc caggcacccg cagttctggc agtcggggtt gccgccgaac tggcacggcg
     4081 tgatccggtg ctcgaaatcg gaggagaagc actcggtcga ctgggcgaag aggcactggt
     4141 cgggcgacgc cggtggtgcg gcaagcgcct tcagcatgcc gtcgcgcatc tgcagcttgg
     4201 ggtagcgcgt gcgaatccgt ccgatctcgg cgatcacgcg ctcgcggtcg gccttcgtga
     4261 ggcgctcagc cgacagctca ccgatctggg gcgtgtagag gctgatccag atcagccgcg
     4321 tgttcgggtt ggcctgccag tagctcacga actcctcgac gtagccgtcc cgcctcgctt
     4381 gctgtcgtgt caccgtgcag tggacggtga tctgatgccc gtcgatgtgc ttgaggatcc
     4441 gctcgtaggt cgccggtgcc cgccgcacat cgtgctccgc cggcagaccg tcgatcgaca
     4501 cgcacacctg caggtgaggc aggccgatcc actcgcgcgg aatcgggcgc acggcgctcg
     4561 tgacgacctg cgtgtggacg ccgcgccgcg ccagctcggg gaggatggtg ttcagctcgc
     4621 gaaaccgcac gagcggctcg ccgccgacga tcgagacgtg caccggcccg tgccgatcga
     4681 tgagcgccat gaaccgagac accagctgct cgcccgtgta atcggacaag cctcgcagcg
     4741 tgacgccccc gccgagatgc tcgtcgccgt acgcgtagca gcccgggcat cgcagcggac
     4801 attccttggt gatctcgatc gagaggtttg gccggtggcc ggcaagaata cggccccacg
     4861 cctggaagac atctcttgtg cgaacgccag tgccagtcga gctcatgtga ccctcattat
     4921 gatctattct tcatacacat gatcggagag ccgcagttgc cgcgaatcgc gcggcgcaag
     4981 tcgaagctgc acgggtgggg cgtcttcgcg ctcgagccca tcaacaagaa ccggcgcatc
     5041 gtcacctaca acggcgaact catcgaccac aaggagagct tcaggcgcga gacgatctac
     5101 ctcgaacgcg gcgagatctg gtgtttcacg gtgaatcgcc gctgggtccg cgacgcgatg
     5161 gtcggcggaa acgttgcccg gttcatcaat cacgcctgca ggcccaactg ttacagtcaa
     5221 atcatcggcc gcgagatctg gattcgcgcc ggacgccgta tcgagccggg tgaagagctg
     5281 acctacgact actgcaccga cggcgaaaag gtcattcagt gcaggtgccg ccctgggtgc
     5341 aagaggcgtc tgtgaaaagc ctgcgaggac tgtgtctcgt cgctctggtc ttgtccgcgg
     5401 cgtatcttgc cgggccggag ttcatcgcga gcgcgcctgg ccagacagac ctcaccgacg
     5461 cggatctcgt gaacgccagc ggcgggcgga ccgtcgccat tggcgcggtc tctggagacg
     5521 gccaggtctc gaggattccg ctcgagctgt acgtggctcg cgtactggcg ggcgagggcg
     5581 agccgaacgc ggcggatgcg gcgcggcagg cgcttgcgat ggcgatacgc acttacacca
     5641 tcgttcagat ggggcgccac cggcgcgaag gcttcgacct ctgcgatacc gtccactgcc
     5701 aggtgcttcg tgccgccaac gagggatcgc gccgcgcggc gctctcgacg gcgggccgcg
     5761 tgctgacgat cgacggcgcc ccggccgagg tgtactactc tgcttcctgc ggagggcgca
     5821 ccgaaggcgc cgcgcaggtc tggccgggag cgacattccc gtacatgcag gtggtcagag
     5881 acgacgacgt gcacgccgag gacgtgccgt ggacgctcga gttcacgctg gccgaactgc
     5941 gcgagacgct cgccagggcc ggcttcggcg ggggggcctt gcgggacgtc gagatcgacg
     6001 gccacacacc ctccggaagg gtcgatcgtc tgcggctgcc cggactgcgc ccggacgcga
     6061 tcgccgcaac cgcgtttcgc gacgcgctcg gcacgacgcg ctttcggagc accgccttct
     6121 cgctcgcccg ggacggcaac atcgtccgct tcaccgggcg aggctacggc cacggcgtcg
     6181 ggatgtgcgt gatcggcgcc ggccggaggg cccgccgcgg tgagtccgcc gaggcaatcc
     6241 tcgcgcagta ctacccgaat ctcacgatca gcagcctggg ccacgccacg gcgccggcac
     6301 ccccgccgcc accgccggcc gatcgcgcgc gcgtcaagcg aatcacggcg gtcgttcccc
     6361 ggggatcgag cgtcggtgcg gatcaactcg aagcgctggc cggccgcgcg tacgagtcgt
     6421 tgtcgcgcgc gctggggacg acggctgcgt cggtgaccgt cgaactgcac gaaaccctcg
     6481 acggcttccg gctggaggcg cgccagccct ggtgggtcag cgctgcggtg acgggcacga
     6541 ccatcgatct cgc
//
LOCUS       DBNGSH010000753         6547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4159241, whole genome shotgun sequence.
ACCESSION   DBNGSH010000753 DBNGSH010000000
VERSION     DBNGSH010000753.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4159241"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="AC16CY_17320"
     CDS             complement(<1..284)
                     /locus_tag="AC16CY_17320"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3355615.1"
                     /translation="MSNLTHYTAHVGSNPAKQFKSTLFRSDHLLLGLNCLEPGQEQHA
                     HVHAGQDKFYFVVEGTGQFVVGGEHRPAGEGMVVWAPAGVSHGVVNRGDRR"
     gene            complement(289..1467)
                     /locus_tag="AC16CY_17325"
     CDS             complement(289..1467)
                     /locus_tag="AC16CY_17325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR3355616.1"
                     /translation="MSTMVSMSRVVINARGEQRIRVGHPWIYRADVVDVQAAGGDAVE
                     VIGPRRRTLGHALYSDRSQISLRMVTRGEAPFDTSVLRARLERAIRFRETLGLDADAY
                     RLVHAEADLLPSLIVDRYGSHLVVQTLSQGMDRLLPEVTGLLTELAGPAGILARNDPR
                     VRGLEGLEQKVEVLAGVVPEMVPVREGPILFDVDLRHGQKTGLFLDQRENREAAARYA
                     RGRLLDCFSYHGGFALRLAGQCAEAEAIDISPEAVARIQANAARNGVSNLRAREANVF
                     DELRRLEKAGERYDTIVLDPPAFAKNKASIPNALAGYKEINLRAMRLLVPGGCLVTCS
                     CSYNVNEELFAAMLHEASIDSHAQVAVVEKRMQGRDHPVLVGVPETHYLKCFVLRKIE
                     "
     gene            1613..2866
                     /locus_tag="AC16CY_17330"
     CDS             1613..2866
                     /locus_tag="AC16CY_17330"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3355617.1"
                     /translation="MMRRALAETDPEIAEAIGNEVRRQSDGLELIASENFVSAAVLEA
                     AGSVMTNKYAEGYPGRRYYGGCEFVDVAESLAISRAKALFKADHANVQPHSGAQANLA
                     VYFAVVKPGDTVLGMNLAHGGHLTHGHPLNFSGQLYTIVPYGVRKDDERIDYDELERL
                     AREHRPKMIMVGASAYPRVIDFARIANVSREVGATMVVDMAHIAGLVATGIHPSPVPH
                     ADYVTSTTHKTLRGPRAGLILCREQYAKEIDKSVFPGVQGGPLMHIVAAKAVCFKEAM
                     EPSFAEYQRQIVANAQALAASLSGHGFRLVSGGTDNHLMLVDVFSKGVTGKAAESALG
                     KAGITVNKNAIPFDQNPPMVASGIRVGTPALTTRGMRQPEMEQIGALMARVLAAPDDG
                     RAIGMVRSEVERLCRRFPLYDPRSG"
     gene            2949..3236
                     /locus_tag="AC16CY_17335"
     CDS             2949..3236
                     /locus_tag="AC16CY_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355618.1"
                     /translation="MAKDDVYSWRISRARKAALEHVAREERVSVAELLDRVTGEWIIS
                     RSAPAGSEDAEQARLRAAASRFIGAIRGGDPDRAAQARQRLRTILKDRRER"
     gene            3226..3645
                     /locus_tag="AC16CY_17340"
     CDS             3226..3645
                     /locus_tag="AC16CY_17340"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3355619.1"
                     /translation="MNANGLIDTGAILALLDRDDRWHSRCAEAFEELRLPLATSAAVL
                     TELFHLLGGERRSRASAWGLLHSGALTVLPIDDRDLVDLESLMKRYADRPMDFADATL
                     VHLARRERLNVILTVDNDDFETYRIEGRRRFRVLPGR"
     gene            3649..5739
                     /locus_tag="AC16CY_17345"
     CDS             3649..5739
                     /locus_tag="AC16CY_17345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR3355620.1"
                     /translation="MNQHSDEGAPEHPRTLAAAAGSVFSDSGPIAHALPGFEPRHGQR
                     RMAAAVASAIEGGATLLAEAGTGTGKTLAYLVPAILSGQRALVSTGTKNLQEQIFFKD
                     LPALREALGVSFTATLMKGRSNYLCLHRWESYRDSADDGVPGRLYDTGERVLLPMIQA
                     WSATTTTGDRAELRDLPEDLPMWKEISAEADTCLGVECPRYGDCFLTLMRQRAAESDI
                     VIVNHHLLCADASVRKSAFGEVIPSCSSLIVDEAHQLEDVATQYFGVSVSNFRVDDLV
                     RDGQRLPGHAGFGEETEEVARALTRVADRARIFFSALSLSKAASPGAAESRARYTPEA
                     ISEYAEDGFMLAGALEGLEATLALMSTGGRGEGAGGSGQGAGGRDQELSDPRSPIPDP
                     AVEVIASLQRRAGELRSDLRFLLRADDPDFVYYFESRGRGMFLRASPVDVSRIVREAL
                     FDRFHAVVLTSATLAVDGSFDYIKGRLGISSSEDLCVPSEFDYRRQALLYLPRRMPPP
                     KSPSFPEAVARETIALLTRSRGRAFVLFTSYRVLREVQPLLQMALPYPTLVQGTAPRS
                     ALLDEFRSTPHAVLLATSSFWQGVDVVGEALSCVVIDKLPFASPGDPVTAARIDAITA
                     RGGDAFSEYQVPLAILSLQQGLGRLIRHRSDQGVLAVLDPRLRTMGYGRRFLASLPPA
                     PLTHDLDAVGRFFK"
     gene            5767..>6547
                     /locus_tag="AC16CY_17350"
     CDS             5767..>6547
                     /locus_tag="AC16CY_17350"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3355621.1"
                     /translation="MRTHLLRVVALLTVVFAVSSSPALAQSLTRGRVIDDQGQPIPDA
                     VILFEATEANRKAQTKTDRKGEFIQIGLASGEYKVTASKEGLGPETQPARIQQGPNAP
                     MTFTLRKAGTTAALAPTEGANKEALAKLQASATAAVAAMKAGNYDEAIAKYNDVLAVA
                     PTCAECHYNIAVSYMNKKDYAQAEAAFKKTIELKPDAADAYTGLAGLYNTQKKFDLAA
                     EASKKAVELSGGGAAGAAAGGGAEASYNQGVIMFNAGKFAEA"
BASE COUNT         1076 a         2219 c         2217 g         1035 t
ORIGIN      
        1 cgccggtcgc cgcggttgac gacgccgtgg ctcacgccgg ccggcgccca caccaccatg
       61 ccttcgcccg cggggcgatg ctccccgccc acgacgaact gccccgtgcc ctcgacgacg
      121 aagtagaact tgtcctggcc ggcgtggacg tgcgcgtgct gctcctggcc gggctcgagg
      181 cagttgaggc cgagcagcag gtgatcgctg cgaaagagcg tggacttgaa ctgcttcgcg
      241 gggttggagc cgacgtgcgc ggtgtagtgg gtcaggttcg acatgaggtc actcgatttt
      301 gcgaaggacg aagcacttca ggtaatgcgt ctcggggacg cccacgagca cgggatgatc
      361 acgcccctgc atgcgcttct cgaccacggc gacctgcgcg tggctgtcga tcgaggcctc
      421 gtggagcatc gccgcgaaca gctcctcgtt gacgttgtac gagcagctgc aggtgacgag
      481 gcacccgccc ggcacgagca gccgcatcgc gcgcaggttg atctccttgt atccggccag
      541 cgcgttcggg atggaggcct tgttcttcgc gaacgcaggc gggtcgagca cgatcgtgtc
      601 gtagcgctcg cccgccttct ccagccgccg cagctcgtcg aacacgttgg cttcccgggc
      661 ccgcagattg gacacgccgt tgcgcgcggc gttggcctgg attcgcgcca ctgcctcggg
      721 agaaatgtcg atcgcctccg cctcggcaca ctgccccgcc agccgaagcg cgaaccctcc
      781 gtggtagctg aagcagtcga gcagccggcc gcgggcgtag cgggccgcgg cctcgcggtt
      841 ctcgcgttga tccaggaaca gaccggtctt ctgcccgtgc cgcagatcga cgtcgaacag
      901 aatcggtccc tcacgcaccg gcaccatctc gggcacgaca ccggcgagga cttccacttt
      961 ctgctcgaga ccctcgagcc cgcggactct ggggtcgttg cgcgcgagga ttcccgcggg
     1021 cccggcgagc tcggtgagga ggcccgtcac ctccggcagc agccggtcca tgccctgcga
     1081 cagcgtctgt acgaccagat gcgagccgta ccgatcgacg atgagcgacg gcagcagatc
     1141 ggcttcggcg tgcaccagcc ggtacgcgtc ggcatcgagc ccgagcgtct cgcgaaaccg
     1201 gatggcgcgc tcgagcctcg cgcggaggac cgacgtgtcg aacggcgcct cgccgcgcgt
     1261 gaccatgcgc agcgagatct gtgagcggtc gctatagagg gcatggccca gcgtgcggcg
     1321 gcgcgggccg atcacctcga cggcgtctcc gccggccgcc tgcacgtcga caacgtcggc
     1381 gcgatagatc cagggatggc cgacgcggat ccgctgctcc ccgcgggcat tgatgacgac
     1441 gcgcgacata gagaccatgg tgctcatgat ggttccgcgg cggctgaagc cgccgctcta
     1501 cgatcgctgg acacgggacc cgtgagagcg tagagcgcac gctttagcgt gcgcggcgag
     1561 cggtcacctg atagtacaat ttcgcgccgt gtccgatccc gccgtgcttc cgatgatgcg
     1621 tcgcgcgctc gccgagaccg atccggagat cgccgaagcc atcggcaacg aagtccgccg
     1681 gcagagtgac gggctggagc tcatcgcgtc ggagaacttc gtcagcgccg ccgtgctcga
     1741 ggccgccggc tcggtgatga cgaacaagta cgccgaaggg tatcccggcc ggcgctacta
     1801 cggcggctgc gagttcgtgg acgtggccga atcgctcgcg atctctcgcg cgaaggcgct
     1861 gttcaaggcg gaccacgcca acgtccagcc gcattccggc gcgcaagcca acctcgccgt
     1921 ctacttcgcc gtcgtcaagc cgggcgacac cgtgcttgga atgaacctgg ctcacggcgg
     1981 gcacctcacc cacgggcatc cgctcaactt ttcaggccag ttgtacacga tcgttccgta
     2041 cggcgtccgc aaggacgacg agcggatcga ttacgacgag ctcgagcggc tcgcgcgcga
     2101 gcacaggccg aagatgatca tggtgggcgc gagcgcgtac ccgcgcgtga tcgacttcgc
     2161 ccgcatcgcg aacgtctccc gggaagtggg cgcgacgatg gtcgtggaca tggcgcacat
     2221 cgccggcctc gtcgcgacgg gcatccatcc gagcccggtg ccgcacgccg actacgtcac
     2281 ctcgacgacg cacaagacgc tgcgcggccc tcgcgccggg ctgattctgt gccgcgagca
     2341 gtacgcgaag gagatcgaca agtccgtctt ccccggcgtg cagggggggc cgctcatgca
     2401 catcgtggcc gccaaggcgg tctgcttcaa ggaagccatg gagccgtcgt tcgcggagta
     2461 tcagcggcag atcgtcgcca acgcgcaggc gctcgccgcg tcgctctcgg gacacggctt
     2521 caggctcgtc agcggcggca ccgacaacca cctgatgctc gtggacgtct tctcgaaggg
     2581 cgtgaccggc aaggccgccg agtcggcgct cgggaaggcg ggcatcaccg tcaacaagaa
     2641 cgcgatcccc ttcgaccaga atcccccgat ggtggcgagc ggcattcgcg tgggcacgcc
     2701 ggcattgacg acgcgcggca tgcgtcagcc ggaaatggag cagatcggcg cgctcatggc
     2761 ccgcgtgctg gccgcccccg acgacggccg cgcgatcggc atggtccggt ccgaagtcga
     2821 gcggctgtgc cgccggttcc cgttgtacga cccgaggtcc ggctgaccgc cttcgccagg
     2881 actcagactc ccgcaccgtc gcgcccgggg ttgggagcgt cgacacaaga tgcactacac
     2941 tgtagtgcat ggccaaggac gacgtgtaca gctggcggat ttcgcgcgcg cgcaaggcgg
     3001 ccctcgagca cgtcgcgcgg gaggagcgcg tcagcgtggc ggagctgctc gatcgcgtga
     3061 cgggcgagtg gatcatttcg cgatcggccc ccgccgggag cgaggacgcc gaacaagccc
     3121 gcctgcgagc ggcagcctcg cgcttcatcg gcgccattcg cggaggagat ccggatcgag
     3181 ccgcgcaggc ccgccagcgc ctcaggacca tcctgaagga ccggcgtgaa cgctaacggt
     3241 ctcatcgaca cgggggccat actggcgctg ctcgaccgtg acgaccgctg gcactcgcga
     3301 tgcgccgagg cgttcgaaga gctgcggctg ccgctcgcca cgtcggcggc cgtcctgacg
     3361 gagctctttc acctgcttgg aggcgagcgc cggagccgcg cctccgcgtg ggggcttctg
     3421 cactctggtg cgctcaccgt cctgccgatt gacgatcgag acctcgtcga cctggagtcg
     3481 ctgatgaagc gctacgcgga tcgaccgatg gactttgcgg atgcgacgct cgtccacctt
     3541 gcccgacggg agcggctgaa tgtcatcctg acggtcgaca acgacgactt cgagacgtat
     3601 cgcatcgagg gccggcggcg gtttcgtgtg ctgccgggac ggtgagccat gaaccagcac
     3661 tcagacgaag gagcacccga gcacccgaga accctggcag ccgccgccgg gtccgtgttc
     3721 tccgattcgg gaccgatcgc gcacgcgctg ccgggcttcg agccgcgcca cggacagcgt
     3781 cgcatggccg ccgccgtcgc ctcggccatc gaaggcggag cgacgctgct ggccgaggcg
     3841 ggaaccggca ccggcaagac gctcgcctat ctcgtccccg cgatcctgag cggtcagcgc
     3901 gcgctcgtct ccacaggcac caagaacctg caggaacaga tcttcttcaa ggatctgccc
     3961 gcgctgcgcg aggcgctggg cgtgtccttc acggcgacgc tgatgaaggg gcgctccaac
     4021 tatctctgcc tgcaccggtg ggaaagctat cgcgactctg ccgacgacgg cgtcccgggc
     4081 cgcctgtacg acacgggcga gcgagtcctg ctgccgatga tccaggcgtg gtccgcaacg
     4141 acgaccaccg gcgatcgtgc cgagctgcgc gacctgcccg aggaccttcc gatgtggaag
     4201 gagatctccg ccgaggccga tacctgcctc ggcgtggaat gtccccgcta cggggactgc
     4261 ttcctcacgt tgatgcgcca gcgcgccgcc gagtccgaca tcgtcatcgt caaccaccac
     4321 ctgctgtgcg ccgacgcgtc ggttcggaaa agcgcgttcg gtgaggtgat tccgtcctgc
     4381 tcttcgctca tcgtggacga ggcgcaccag ctcgaggacg tggcgacgca gtacttcggc
     4441 gtctcggtca gcaacttccg cgtggacgac ctggtccggg acggccagcg cctgcccggc
     4501 cacgccgggt ttggagagga gacggaagag gtcgcgcggg cgctgacgcg cgtggccgat
     4561 cgtgcccgga tcttcttctc ggcgctctcg ctgtccaagg cagccagtcc gggcgccgcg
     4621 gagtcccggg cccgatacac gcctgaggcc atctccgagt acgcggagga cggcttcatg
     4681 ctggccggcg cgctcgaagg gctggaggca acgctggcgc tgatgtcaac agggggcagg
     4741 ggggaggggg cagggggcag cgggcagggg gcagggggca gggatcaaga gctttctgat
     4801 ccccgatccc caatccctga tcccgccgtc gaagtgattg cgtccctcca gcgtcgcgcc
     4861 ggagagctgc ggagcgatct gcggttcctg ctgcgtgccg acgatccgga cttcgtctac
     4921 tacttcgaaa gccgcggacg cgggatgttt ctccgcgcgt cgcccgtcga cgtctcgcgc
     4981 atcgttcgcg aggcgctgtt cgatcgtttt cacgccgtgg tgctcacgtc ggccacgctc
     5041 gccgtggacg gatcgttcga ctacatcaag gggcggctcg gcatcagcag ctccgaggat
     5101 ctctgtgtcc cgtccgagtt cgactaccgc cggcaggcgc tgctgtacct cccccgacgc
     5161 atgcccccgc cgaagtcgcc gtcgtttccc gaggcggtcg cacgcgaaac gatcgcgctg
     5221 ctgacacgct cgcgcgggcg ggcgttcgtg ctgttcacca gttacagggt cctgcgggag
     5281 gtacagccgc ttctccagat ggccctgccc tatccgacac tggtccaggg caccgctcct
     5341 cgatcggcgc tcctcgatga gttcaggtcc acgccccacg ccgtgctcct ggccacctcg
     5401 agcttctggc agggggtaga cgtcgtgggc gaggcgctga gctgcgtcgt catcgacaag
     5461 ctgccctttg cctcgcccgg cgaccccgtc acggccgcgc gaatcgacgc gattacggcc
     5521 cgcggcgggg atgcgttttc ggagtaccag gttccccttg ccatcctctc gctgcagcag
     5581 gggctcgggc gattgatcag gcaccgcagc gatcaaggcg tgctcgcggt gctcgatccc
     5641 cggctgcgga cgatgggata tggacggcga ttcctggcgt cgcttccccc cgcgcccctt
     5701 acacacgacc tcgatgcagt tggccggttc ttcaagtaga cttgtgtcgt gattcaggag
     5761 gctctcatgc ggacccatct gctccgcgtc gtcgcgctcc tcacggttgt gtttgcggtg
     5821 tcgtcgtcgc ctgcgctggc gcaaagcctc acgcgcggca gggtcatcga cgatcagggt
     5881 caaccgattc ctgacgccgt catccttttc gaagccacag aggccaaccg caaggcccag
     5941 acgaagaccg atcgcaaggg ggagttcatc cagataggcc tggcgtccgg cgaatacaag
     6001 gtgacggcct cgaaggaagg cctcggcccc gagacgcagc cggcgaggat acagcagggg
     6061 ccgaatgcgc cgatgacctt cactcttcgc aaagcgggaa cgacggcggc gcttgcgccc
     6121 acggagggcg caaacaaaga ggcgctcgcg aaactgcaag cgtcggcgac ggccgcggtg
     6181 gcggccatga aggccgggaa ttatgacgag gcgatcgcca agtacaacga cgtccttgcc
     6241 gtggccccga cgtgcgcgga gtgccactac aacatcgctg tcagctacat gaataagaag
     6301 gactacgcgc aggccgaagc ggcgttcaag aagacgatcg agctgaagcc cgacgccgcc
     6361 gacgcctaca ccggccttgc gggcctgtac aacacgcaga agaagttcga cctcgcggcc
     6421 gaagccagca agaaagccgt ggagctttcg ggcgggggcg cggccggggc cgcggccgga
     6481 ggcggcgccg aagccagcta caaccagggc gtcatcatgt tcaacgcggg caagttcgcc
     6541 gaggcga
//
LOCUS       DBNGSH010000754         7372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_83292, whole genome shotgun sequence.
ACCESSION   DBNGSH010000754 DBNGSH010000000
VERSION     DBNGSH010000754.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_83292"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="AC16CY_17355"
     CDS             <1..525
                     /locus_tag="AC16CY_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355622.1"
                     /translation="GSASTRADSPEDRAQAERCLTTDGLLVPNPFYNNYHQIFQAPGY
                     VAILTEMMHEARIIPLDRRPHAGSGVRMWMGDSRGHWEGKTLVVETTNFNDRKLFRGA
                     TGQMTLVERFTRLDADTIEYRLTVTDPATFSQPWTLENGLHKASGALYEVDCHEGNYG
                     LRGILSGARAQETK"
     gene            573..839
                     /locus_tag="AC16CY_17360"
     CDS             573..839
                     /locus_tag="AC16CY_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HIR3355623.1"
                     /translation="MPKHERVARRSGAAAGRRPIDGTAAKMRRYRQRLRAAGLRPLQI
                     WVPDTTVPGVRRSLRTQSIRASAHPSNEDAQPFLDAAFLDQLDE"
     gene            836..1192
                     /locus_tag="AC16CY_17365"
     CDS             836..1192
                     /locus_tag="AC16CY_17365"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR3355624.1"
                     /translation="MTKGAEAAPMTRGDICLVAVPGDYGKPRPAVVVQTDLLNATHAS
                     LIVCLLTSRFMNAPAFRLDVDATTSTGLQARSQIMVDKVATVRRDRVRERIGALDDGT
                     RLRLDRALTLVLGLAG"
     gene            complement(1287..1700)
                     /locus_tag="AC16CY_17370"
     CDS             complement(1287..1700)
                     /locus_tag="AC16CY_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355625.1"
                     /translation="MFGGSIAGEQGRTVADAHGIKAVHITQSADTMWLRFAPPAAARI
                     LHIQARHDDGADLTPYIRTGIARWDGTGLLVVSGFPTLALWTRPLTRGVGPLIERLSL
                     GAGGTLKYTAFYDDSPMPSPPPRAVELWRCSGATD"
     gene            1835..2167
                     /locus_tag="AC16CY_17375"
                     /pseudo
     CDS             1835..2167
                     /locus_tag="AC16CY_17375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2409..2639
                     /locus_tag="AC16CY_17380"
     CDS             2409..2639
                     /locus_tag="AC16CY_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355626.1"
                     /translation="MFRAVIRQSTTPAPPEDLLNVVDRRTLAELLVASGGTSTRLPGN
                     EHEFLERQRFIDNQAALAAVQAALVSLRNPGQ"
     gene            complement(2773..3381)
                     /locus_tag="AC16CY_17385"
     CDS             complement(2773..3381)
                     /locus_tag="AC16CY_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3355627.1"
                     /translation="MATQRTISPGEHVPTADQRVVTYGVPWAHYEAQLALRGDASVPR
                     MAYLEGALELMSPSKDHERVTSYLGRLVEAYALERGIDFSPYGSWTLKSGPKQSGAEP
                     DECYIIGPDQNKDVPDLAIEVVWTSGGLDKLEIYRRLGVGEVWIWKDGRITVHSARAG
                     AFEQVDRSRLFPDLNLDLLCSLLDRPTASQAVKALREALRSA"
     gene            complement(3465..6932)
                     /locus_tag="AC16CY_17390"
     CDS             complement(3465..6932)
                     /locus_tag="AC16CY_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HIR3355628.1"
                     /translation="MPADPPSSASPDQAPQGRAAQLWNQAKAGLFPENETTDFEQLYK
                     TETKQLDEADSTPADIAEQSRAEKHDKDRGRYEPQINTGVAGEPWVSFTARDRLGLAL
                     SGGGIRSATFNLGLLQSLARLGILRHVHYLSTVSGGGYVGGFWTAWLARRGMKAGESH
                     FPLGGDHRGGERAEVRHLREFSRFLLPRMGLRQTEFWAVVMTIVGGLLPSLVAALAVL
                     VIAWSVWIVLVWALAGPDPWGYLWFGAAFAAFFLVTELFWRRTNKALWADDKTTRSDI
                     VEWVGYGGGALVSVAAVVWMWMLRDSWLPDALLSDQRLPLGTALFGPSVVLVACFVGL
                     VGIRTLVVRFARGSSGHGLSFLVGIERSLSRVAAMTALSTALSIAGWATALLQNFAIP
                     GTIGTAGLASLFAWARKWLAEPIKETRGTKLLDMATAWLKRATPRVLATLVLILLFLL
                     VGIGLRWILQDGNVNWQRYSLALGTSLGILLLTAWWFDPARVGLHEFYRSRISRCYLG
                     ASNTEGFKTERDRVARNRHVSERPGDDLTLGQLRDAKVRPIHLVCTAANDLSGDHLAT
                     LYRGAKSAVLSVNGISIGNQAAPLDYVRFSAALTASAAAFNSEMGRVSMNLGPAVAFL
                     MSTLNLRLGLWVPHPQNPYRSDYWLPGRFFIFELLGLSRTDRQHLHLSDGAHFENLGL
                     YELVRRHCRYIIVSDCGEDQSVTFDDLANVLRRVREDFGVEIEVDVDPLRPDEDGIAR
                     QHAVMGTIHYDGYNGVDKGTLLLFKPSLTGDEPPDVLQYRSRNTRFPHESTGDQFYDE
                     AQWESYRRLGEHAGYVSLAFSESDVHENPDFTDRLFVGIRERLHPAPEGNEEIFIQMS
                     ERCAALESDVFNSGPQGLRREFFPEAVAVSGEGSADLDEEVQGLSYMMRALQIMEDVW
                     VSADLDRYWSHPLNEGWMNYFQRWASTPTFRKWWPILRPIYSSGFRDFAKERFGITVR
                     EKPAGAAPDPSTIRLRLEPAPTLDAFMRGHAWHQFVQRAVAPDLNDLVTLAYRMEFAL
                     DGGSAATRTLDVGFVLIKEKPAKDTWSATWHAHHFYVPPPLVGAGIIGRQLDAVIAHY
                     KAQASAQPPRAFDRLTVVFGEDSTPPPRGRAAREERVRQIEFFKSRGFQHERQGNPTL
                     GQVRLTLTL"
     gene            6939..>7372
                     /locus_tag="AC16CY_17395"
     CDS             6939..>7372
                     /locus_tag="AC16CY_17395"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF019931.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HIR3355629.1"
                     /translation="MVNLVGPDMVTSAHIKSKAAALGFDLCGIAPADDFPELSFLDEW
                     LEKGYAGEMAWMRRTAERRSDVRNVVPGARSVIVTATLYNTDRPYGHELPAARSQEPE
                     ARAGISRYAWGDDYHGVISRRLDALLEWMRSTSGVPFDARAYV"
BASE COUNT         1283 a         2460 c         2426 g         1203 t
ORIGIN      
        1 gggtcggcgt caacccgcgc cgacagcccg gaggatcgcg cgcaggccga gcgctgcctc
       61 acgacggacg gcctgctcgt gccgaacccc ttctacaaca actatcacca gatcttccag
      121 gcgccaggct acgtcgcgat cctgacggag atgatgcacg aggctcgcat cattccgctc
      181 gatcgacgcc cccacgcggg ctcgggcgtt cgcatgtgga tgggcgattc gcgaggccac
      241 tgggagggca agacgctcgt cgtcgagacg accaatttca acgacaggaa actctttcgc
      301 ggcgcgacgg gacagatgac gctcgtcgag cgcttcacgc gcctcgatgc cgacacgatc
      361 gagtaccgcc tcaccgtgac cgatccggcg accttttcgc agccgtggac gctcgagaac
      421 ggactgcaca aggccagcgg tgccctctac gaggtggact gccacgaagg caactacggc
      481 ctcaggggga tcctgtcggg ggcgcgggcg caggagacga agtagctggc cccaagcgcg
      541 tcgttcacac ttctccgtgg taacatgtga ccatgcccaa gcacgagaga gtggcacggc
      601 gttccggcgc cgcggcaggg aggcggccga tcgacggtac ggctgccaag atgcggcgat
      661 atcgccagcg gctgcgcgct gccggtctgc ggccgcttca gatttgggtg cccgacacga
      721 cggtgccggg cgttcggcga tcgctgcgca cccaatccat tcgcgcgtcg gcgcatccct
      781 ccaatgaaga cgcgcagccg tttctcgacg cggccttcct ggatcagttg gacgaatgac
      841 gaagggagcc gaagccgcgc cgatgacgcg cggcgacatc tgtctcgtgg ctgtcccggg
      901 tgactacggc aagccacgcc cggccgtcgt ggtacaaacc gaccttctca atgcgaccca
      961 cgccagtctc atcgtgtgcc tgttgacgag ccgcttcatg aacgctccgg cgttccgcct
     1021 cgacgttgat gccacgacct cgacggggct ccaggcgcgc tcccagatca tggtcgacaa
     1081 ggtcgcgacc gtgagacggg accgcgttcg ggagcgaatc ggcgccctcg acgacggcac
     1141 tcgccttcga ctcgaccgag ccctcaccct ggtgctcggc ctcgcaggct agtgcctctt
     1201 tgcagggtgt catcaatcgc cgatcagcga cgaagcgaca atcaacagtc aaagatcgag
     1261 caaggcgatc cctgccccag ctcgaatcag tccgtggcgc cgctacaacg ccaaagctcg
     1321 accgcacgag gcggcggact gggcatcggc gagtcgtcat agaacgccgt gtacttcaac
     1381 gtcccgcctg ctcccagcga caagcgttcg atcaggggac cgacaccccg cgtcaaggga
     1441 cgcgtccaca gcgcgagtgt gggaaaaccg gatacgacga gcagcccagt gccatcccat
     1501 cttgcgatgc cggttcggat gtacggcgtc aggtcggctc cgtcgtcatg cctggcttga
     1561 atgtgcagga ttcgagcggc ggccgggggc gcgaatcgca gccacatcgt gtcggccgac
     1621 tgcgtaatgt gaacggcttt gatcccgtgc gcatcagcga ctgtcctccc ctgctcaccg
     1681 gcgattgagc caccgaacat ggctcagctc cgactgcgca gcaagcgccg tacgatctgc
     1741 ctgccgtacc tatttcgtgc actgaacggt cagcgttgcc tcaaatcgtg tatctgcttt
     1801 gttagactgc cgtgcctgcg aaaggaggaa cagggtggca catcggatcc tgcttgtcga
     1861 tcacagccgg gagaccctcg atgccatggg agcggtcctc agtcgagccg gccatcaggt
     1921 cgcgcgggcg cagacggcgc gcgacgcact gcgactggcc ggtaagttca agcctgacgt
     1981 ggcgctcatc gatctcgagt taccggacac gcccgggccc gaattgattc gtatgctgcg
     2041 tcagacacac gacggcctct cgtgcgtgct catcacgcgc ttcgggcacg aggagtcaga
     2101 cgcggaggca ctccgtctgg gtgcgtccga ttgcctgcgg cagccggtga cccgaagcct
     2161 gctgctggcc gcggtggaaa gcgcggccgg aggcacggga tgcaacggaa cagatcacgc
     2221 cgggctccag gcggaatgcc actcccttac cagatgggcg gacgtcgtcg ttcgcagcct
     2281 ggccgctcct cgggactttc gcacgctccc ggagttcggg cgcgcccgtg ggtgtgtcga
     2341 gcggcgggtt ccgcaactgg tgccgcactg cccacttatc accaaggcag tcgctcaagt
     2401 tcgcgcgcgt gttccgggcg gtcatccgac aatcgaccac gccggccccg cccgaagacc
     2461 tgctgaacgt cgtcgaccgc cggacgctgg ccgagctgct cgtcgcgtcg gggggaacgt
     2521 ccacgcgcct gccgggcaac gagcacgagt ttctcgagcg ccagcgcttc atcgacaacc
     2581 aggcagcatt ggcggccgtt caggcggcgc tggtgtcgct gcgcaaccct ggacaatgac
     2641 cgtcgcttcg tcactgagga tgaattgccg acgtcgccat cgagatcggc gcgacgatga
     2701 cggtcgatga cgtcacggaa gaggagttaa gggcggtgct ccttcgcccg gggctgaagg
     2761 gtcaggaggc agctacgcgg agcgcaaggc ctctcgaagc gccttcacgg cctgtgacgc
     2821 ggtgggtcga tccagcaacg agcagagcag gtccagattg aggtcgggaa acagccggct
     2881 ccggtccacc tgctcgaatg cgcctgcacg cgccgagtga accgtgatcc tgccgtcctt
     2941 ccagatccag acctcgccga cgccgagccg gcgatagatc tccagcttgt cgaggccgcc
     3001 gctcgtccac acgacttcga tcgcgaggtc cggcacgtcc ttgttctggt cggggccgat
     3061 gatgtagcac tcgtctggct cggcgccact ttgtttcgga ccactcttga gcgtccagct
     3121 tccatacggc gagaagtcga tgccgcgttc gagggcgtat gcctcgacga ggcgcccgag
     3181 gtacgatgtg acacgctcgt ggtccttcga agggctcatg agctcgagcg ctccttccag
     3241 gtaggccatc cgtggaaccg acgcatcgcc ccgcaaggcg agttgcgcct cgtagtgcgc
     3301 ccacggcacc ccgtaggtga cgacccgctg gtcggcggtc ggcacgtgct cgccggggga
     3361 aattgtcctc tgtgtcgcca cggctcaatc ctaccacccg gacccccgac acgagcgcag
     3421 aaggttcccc tgaagcgaga agtgtgaacg agggttctac agttctacag cgtgagcgtc
     3481 aacctgacct gaccgagcgt cgggttcccc tgccgctcgt gttgaaagcc gcggctcttg
     3541 aagaactcga tctgacggac ccgctcctct cgggcggcgc gtccccgcgg cggcggcgtg
     3601 gagtcctcgc cgaagacgac tgtcagccga tcgaaagcgc ggggcggttg tgccgatgcc
     3661 tgagccttgt aatgggcgat gaccgcatcg agctggcgcc cgatgatgcc ggcgccaacg
     3721 agcgggggcg gaacgtagaa atggtgggca tgccacgtgg ccgaccaggt atctttcgcc
     3781 ggtttctcct ttatcagcac gaacccgacg tccagcgtcc gcgtcgcggc cgagcccccg
     3841 tcgagcgcga actccatgcg gtaggcgagg gtcacgagat cgttcagatc gggcgcaact
     3901 gcgcgctgca cgaactggtg ccacgcatgt ccccgcatga aggcgtccag cgtcggcgcg
     3961 ggctcgaggc gcaagcggat cgtggacggg tcaggcgccg cgcccgcggg tttctcccgg
     4021 accgtgatgc cgaagcgctc cttcgcgaaa tcgcggaagc cgctgctgta gatcggccgc
     4081 aggatcggcc accacttgcg gaacgtcggc gtggacgccc agcgctggaa gtagttcatc
     4141 cacccttcgt tgagcggatg cgaccagtag cggtcgagat cggctgaaac ccagacgtct
     4201 tccatgatct gcagcgcacg catcatgtag ctgaggccct gtacttcctc gtccagatcc
     4261 gcggagccct cgccgctcac ggcgaccgcc tccgggaaga actcgcggcg caggccctgc
     4321 ggaccgctgt tgaagacgtc ggattcgagc gcggcgcacc gctcgctcat ctggatgaag
     4381 atctcttcgt ttccttcagg agccgggtgg agccgctcgc ggatgccgac gaagagccgg
     4441 tcggtgaagt ccgggttctc gtggacgtcc gactccgaga acgccagcga cacgtagccg
     4501 gcgtgttcac cgagccgccg gtacgattcc cactgcgctt cgtcgtagaa ctgatcgccc
     4561 gtggattcgt gcgggaaccg cgtgttcctg gagcggtact gcagaacgtc gggcggctcg
     4621 tctcccgtca gcgacggttt gaagaggagc agggtgccct tgtccacgcc gttgtagccg
     4681 tcgtagtgga tcgttcccat gaccgcatgc tgccgggcga ttccgtcctc gtcgggacga
     4741 agcggatcca cgtccacctc gatctcgacg ccgaaatctt ccctgacgcg ccgcaggacg
     4801 ttggccaggt cgtcgaaggt cacggactga tcctccccgc agtccgagac gatgatgtag
     4861 cggcagtggc ggcggacgag ctcgtagagg cccaggttct cgaaatgggc gccgtcggag
     4921 aggtggaggt gctgacggtc cgtccgcgac aggcccagca gctcgaagat gaagaaccgg
     4981 ccagggagcc agtagtcgct ccggtacgga ttctgcgggt gcggcaccca caggccgagg
     5041 cgcaggttga gcgtgctcat gaggaacgcg acggccggac ccaggttcat cgacacgcgg
     5101 cccatctcgg agttgaacgc cgcggccgac gccgtcagcg ccgccgagaa ccgcacatag
     5161 tcgaggggcg ccgcctggtt gccgatgctg atcccgttca ccgacagcac cgcgctcttg
     5221 gcgccgcgat acagcgtcgc caggtggtcg cccgacagat cgttggccgc cgtgcacacc
     5281 agatggatcg gcctcacctt ggcgtcccgc agctgcccga gcgtcagatc gtcgcctgga
     5341 cgctcgctga cgtgacggtt gcgcgccacg cgatcgcgct cggtcttgaa gccctcggtg
     5401 ttcgacgcgc cgagatagca gcgcgagatg cgcgagcggt agaactcgtg gaggccgacg
     5461 cgcgcgggat cgaaccacca cgcggtcagc agcaggatac cgaggctcgt tccgagcgcc
     5521 aggctgtatc gctgccagtt gacgttgccg tcctgcagga tccaccgcag tccgatgccc
     5581 accagcagga acagcagaat caagacgagc gtggcgagca cgcgcggcgt cgcgcgcttg
     5641 agccaggccg tagccatgtc gagcagcttc gtcccgcgtg tttccttgat cggctctgcc
     5701 agccacttcc tggcccacgc gaacaacgac gccagacctg ccgtgccaat ggtgcccggg
     5761 atcgcgaaat tctgcagtag cgctgtcgcc cagcctgcga tcgacagcgc cgtcgagagg
     5821 gccgtcatgg cggcgacgcg actgaggctt cgctcgatgc cgacgaggaa cgacaggccg
     5881 tggccggagg accctcgggc gaagcggacg acgagcgtgc gaatgccgac cagtccgaca
     5941 aagcacgcaa cgagtacgac cgagggcccg aacagtgccg tgccgagcgg caaacgctgg
     6001 tcgctcaaca gggcatccgg caaccacgag tcccggagca tccacatcca gaccaccgcc
     6061 gcgacgctca cgagcgcgcc gcctccgtag cccacccatt cgacgatgtc actgcgagtc
     6121 gtcttgtcgt cggcccagag cgccttgttc gtccgccgcc agaacagctc ggtgaccagg
     6181 aagaacgccg cgaatgccgc tccgaaccag aggtaccccc acgggtcggg gccggcgagc
     6241 gcccagacga gaacaatcca gacgctccat gcgatcacca ggacggccaa cgccgcgacg
     6301 agcgacggca gcagcccgcc gacgatcgtc atcacgacgg cccagaattc ggtctggcgg
     6361 agtcccatcc gcggcagcag gaagcggctg aactcccgca ggtgacgtac ttcggcgcgt
     6421 tccccgccgc gatggtcgcc gccgaggggg aaatgggatt cgccggcctt catgccccgc
     6481 cgcgcgagcc aggcagtcca gaagccgccg acatagccgc cgcccgacac ggtggacagg
     6541 tagtggacgt gccggaggat gcccagtcgc gcgaggctct gcagcaggcc gaggttgaag
     6601 gtggcgcttc ggatgccgcc gccggagagc gccagtccca ggcgatcgcg cgccgtgaac
     6661 gagacccacg gctcgccggc caccccggtg ttgatctgcg gctcgtagcg acctcggtct
     6721 ttgtcgtgct tttcggcgcg gctctgctcg gcgatgtcgg ctggcgtgga gtctgcctcg
     6781 tcgagctgtt tcgtttccgt cttgtacagt tgttcgaagt cagtcgtctc gttttcaggg
     6841 aagagcccgg ccttcgcctg gttccacagt tgcgcggcgc gcccctgcgg ggcctgatcc
     6901 ggagacgcag atgacggagg atcggcgggc atcggagcat ggtaaacctt gtcggaccgg
     6961 acatggtgac gtctgcacac atcaaatcca aggcagccgc gctcggcttc gacctgtgcg
     7021 gcatcgctcc ggcggacgac tttccggagt tgtcttttct ggacgagtgg ctggagaagg
     7081 gctatgccgg ggagatggcc tggatgcgcc gcaccgccga acggcgcagc gacgtgcgca
     7141 acgtcgttcc gggcgcccga agcgtcatcg tcaccgccac gctgtacaac accgatcgcc
     7201 cgtacggcca cgagctgccg gcagcccgga gccaggagcc cgaagcccgc gccgggatct
     7261 cccgctacgc ctggggcgac gactaccacg gcgtcatctc gcgccgtctc gacgcgctgc
     7321 tcgagtggat gcgatcgacg tccggcgtcc cgttcgacgc gcgcgcgtac gt
//
LOCUS       DBNGSH010000755         5430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_423298, whole genome shotgun sequence.
ACCESSION   DBNGSH010000755 DBNGSH010000000
VERSION     DBNGSH010000755.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_423298"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..2349
                     /gene="lpxK"
                     /locus_tag="AC16CY_17400"
     CDS             49..2349
                     /gene="lpxK"
                     /locus_tag="AC16CY_17400"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR3355630.1"
                     /translation="MYLLYSVAILIALVVLSPYFLYQALRHHKYVGSLRQRLGYLPVS
                     FNLDAEESIWVHAVSVGEVLAARPIVTDLRARHPDLRVFLSTTTLSGQNLARRSVPDA
                     DGVFYLPFDWAFTVRRTLDVVKPKLLVLVETEIWPNLLRECRRRGVRTALVNGRISAR
                     SFPRYRVIRPFIRRVLADIERLCVQGEETASRLRQLGADPGRMTTTGSLKFDSLDPST
                     APGRGRERVLRFFRLTAGRQVLVAGSTSKGEEEAVIRAFNRVRSSAAGGNALLVLAAR
                     HPERFGEVERLCQREGLSTARRSDLPIDAEPRVDAVILDTIGELAQLYQIATAVFVGG
                     SLVPAGGHNILEPAVHGKPIVFGPHMENFSEIAGAFLAHGAAVQVRTPRELEDTFVAL
                     MADPVRRARLGAAARARGRQPRRQGQDPERAGRFVPSARTDARGRPPLPRDPLTLSAV
                     YGRVARLRRSWYERHPQSRRALDSTVISVGNLSVGGSGKTPVVAALARLLLDIGQRPA
                     ILSRGYGRPRALDGVVVASDGDRVLEPVERTGDEPQMLARALKGVPVLVSPDRHVAGR
                     LAERRFGCTVMLLDDGFQHLELRRDVDLLLVSCADLDDRVLPAGRLREGLDAARSADC
                     LLVPGPPEEVARVSAAFDGMQVFSVVNHYRPLRPLDETASLPAGLRRVAAAAGIARPQ
                     RFFSALREQGFDVAREIVFSDHHWFTTGDVESVGRAARDAGAELIVTTEKDAARLPPL
                     PGWATLPMVAAIEPEGQFRSWLQGRL"
     gene            2346..3260
                     /locus_tag="AC16CY_17405"
     CDS             2346..3260
                     /locus_tag="AC16CY_17405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3355631.1"
                     /translation="MRYRLEYALVMLIAAAVRLMPLTIVRACGRAFGRLVSHIDRSHR
                     RIAIDNLARAFPSRSPRECRAICGAMFAHFGSLLFELIKFSTLGREDMLARVDIEGDE
                     HLRQAYQRGRGVFILTGHFGYWEIHGLVTALHMRPIGVLARPLDNPYLHAMLERIRTR
                     TGNTVIYRQGALRKMLRELGQNEGVALMIDQHLHSDTVSVDFFGRRAATTSALAALAI
                     RTGAAVIPAFSLPLPNGRYRLVYERPVPLPPEDAPDAVAELTQRCTDVLEMYVRRDPH
                     LWLWMHRRWRDGVRPHLAPDEGAAENGV"
     gene            3250..3834
                     /locus_tag="AC16CY_17410"
     CDS             3250..3834
                     /locus_tag="AC16CY_17410"
                     /EC_number="3.1.3.83"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034200 - D-glycero-beta-D-manno-heptose
                     1,7-bisphosphate 7-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-alpha-D-manno-heptose-1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR3355632.1"
                     /translation="MGSRRAVFLDRDGTIIEERGYIDRLDMLSLFPWTGDALRLLKRA
                     GLATVVITNQSAVGRGIIDEAFLDTVHQEINRRLQPSGAAIDGYYYCPHFADSTIERY
                     RRECRCRKPSPGLVEQACREMDLDPARSVMVGDRWIDVACARAAGTRAVLVRSGHGRH
                     EEAVPPKDVRADAILDNLMEAVGWILRGAERTGG"
     gene            3827..4189
                     /locus_tag="AC16CY_17415"
     CDS             3827..4189
                     /locus_tag="AC16CY_17415"
                     /inference="COORDINATES: protein motif:HMM:NF013621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIR3355633.1"
                     /translation="MGEVVSLEQLLDRVRGLRQTGLTIALADGTFDLLTAGHVRYLES
                     TAQEADLLVVGIASDATVTDLEGEGRPILAAEHRAALVAALRCVDFVVVYSEPTAGPL
                     AEALLPDVHRTGGSRTES"
     gene            4186..5208
                     /gene="waaC"
                     /locus_tag="AC16CY_17420"
     CDS             4186..5208
                     /gene="waaC"
                     /locus_tag="AC16CY_17420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HIR3355634.1"
                     /translation="MTERPRRFLVVRLGSMGDVIHAIPAVASLRARHAAARIDWLVDP
                     RYAAVPQMVRGVDQAIPIDTRGSAIDLLRTIRRLRQAGYDAAVDLQGLIKSAVLARAA
                     GARRTLGFPRERLREPIARVFYTETHDPGRDAHVISKNLGLMRALGVEDVQVTFPLNV
                     PRTPAGDNAAAAFGPDGYAVIHPGAAWPNKRWPAERFGAVAAGIRARIGVRSLVLWGP
                     GEESLASLVAAASKGCADVSPRTSIVDLFAIVRGAKLMIAGDTGPLHVAASVGTPIVG
                     LFGPTLAERSGPYSRADIALSRTSRCSCRDERRCRLPQPCIDDIGVEEVVAAVERRLA
                     QWPNPQ"
     gene            5205..>5430
                     /locus_tag="AC16CY_17425"
     CDS             5205..>5430
                     /locus_tag="AC16CY_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HIR3355635.1"
                     /translation="MIDFLARKRVTLGFVVALAVAFFARPTWESWRIGLLVAGVGEGL
                     RIWAAGHLEKGREVTRSGPYRWTAHPLYVGS"
BASE COUNT          748 a         1875 c         1894 g          913 t
ORIGIN      
        1 cggggctata cttcgtgatt gtaaggcgca cattccacac cgcttagcgt gtacctcctc
       61 tacagcgtcg ccatcctgat cgcgctcgtc gtgctctcgc cgtattttct gtatcaggcg
      121 ctgcgccatc acaagtacgt cggcagcctt cgacagcggc tggggtacct gccggtgtcg
      181 ttcaacctcg atgccgagga atcgatctgg gtgcatgcgg tgtcggtggg cgaggtgctg
      241 gcggcccgcc cgatcgtgac ggaccttcgc gcgcgtcatc cggacctgcg cgtcttcctg
      301 tcgacgacca cgctctcggg gcagaacctg gcgcgccgca gcgtgcccga tgccgacggt
      361 gtgttctatc tcccgttcga ttgggcgttc accgtccgtc ggacgctgga cgtcgtgaag
      421 ccgaagctgc tcgtcctcgt cgagaccgag atctggccga acctgctccg ggagtgccgc
      481 cgccgcggcg tgcggacggc gctcgtgaac ggccgcatct cggcgcggtc gttcccgcgc
      541 taccgcgtca tccgcccgtt catcaggcgg gtgctcgctg acatcgagcg cttgtgcgtg
      601 cagggcgagg agaccgcgag ccggctccgc cagctcgggg cggatcccgg tcgcatgacg
      661 acgacaggca gcctcaagtt cgactcgctc gatccgtcaa cggcccccgg acgcgggcgg
      721 gagcgcgtgc tgcgattctt ccgcctcacg gccggccggc aggtgctggt ggcgggcagc
      781 acctcgaagg gggaagaaga ggccgtcatc cgcgccttca accgcgtccg ctccagtgcc
      841 gccggcggca atgccctgct ggtccttgcg gcacggcatc ccgagcgatt cggcgaggtg
      901 gagcggctgt gccagaggga agggctgtcc acggcgcgcc gcagcgacct gccgattgat
      961 gccgagccgc gggtggacgc cgtgatcctc gacacgattg gagagctcgc ccagctctat
     1021 cagatcgcga ccgcggtctt cgtcggtggc agcctcgtgc cggccggcgg gcacaacatc
     1081 ctcgagccgg ccgttcacgg caagccgatc gtcttcggtc ctcacatgga gaacttctcc
     1141 gagatcgccg gcgccttcct ggctcacggc gcggccgtgc aggtgcgcac gccgcgggag
     1201 ctcgaagaca cgttcgtcgc gctgatggcc gatcccgtgc gccgggcgcg tcttggggcg
     1261 gccgcgcgcg ctcgtggacg ccaaccgcgg cgccaaggac aagaccctga acgtgctggc
     1321 cgatttgttc cctccgcgcg gacggacgcc cgcggtcgtc cgccccttcc gcgtgatcca
     1381 ttgaccctta gcgccgtcta tggacgggtg gccaggctgc ggcgctcgtg gtacgagcgg
     1441 cacccgcaat cccggcgtgc gctggacagc accgtcatca gcgtcggcaa cctgtcggtg
     1501 ggcggcagcg ggaagacgcc ggtcgtggcg gcgctcgcgc gcctgctgct cgacatcggc
     1561 cagcgcccgg cgatcctgag ccgcggctac ggccgtccac gcgctctcga cggcgtcgtc
     1621 gtggcgagcg acggagaccg cgtcctcgag cccgtcgagc gcaccggcga cgagccgcag
     1681 atgctggctc gcgcgctgaa aggcgtgccc gttctggtgt ctccggaccg tcacgtggcg
     1741 gggcgcctcg cggaacgcag attcggctgc acggtgatgc tgctcgacga cgggtttcaa
     1801 cacctggagc tccgccggga cgtggatctg ctgctcgtct cctgtgccga cctcgacgat
     1861 cgagtgcttc ccgccggtcg tctgcgcgag gggctcgacg ccgcccgctc ggcggactgt
     1921 ctgctggtgc cggggccgcc cgaggaggtg gcccgcgtgt cggcggcctt cgacggcatg
     1981 caggtattca gcgtcgtgaa tcattaccgt cccctccgcc cgctcgatga gacggcctcg
     2041 cttcccgccg gactccggcg tgtcgccgcc gcggcgggca tcgcccggcc gcaacgcttt
     2101 ttcagcgcgc tgagggagca gggcttcgac gtggcgcggg agatcgtgtt ctccgatcac
     2161 cattggttca cgacaggcga cgtcgaaagc gtcggccgcg ccgcgcgcga cgcgggcgcc
     2221 gagctgatcg tgacaacgga gaaggacgct gcccggctgc cgcccctgcc cggttgggcc
     2281 acgttgccca tggtggccgc gatcgagccc gaggggcagt tccgatcgtg gctccagggc
     2341 cggctgtgag gtaccgcctc gagtacgcgc tcgtcatgct catcgccgcc gctgtgcggc
     2401 tgatgccgct gacgatcgtg cgggcgtgcg gacgggcgtt cgggcgcctg gtgtcccaca
     2461 tcgatcgctc gcaccgcagg attgcgatcg acaatctcgc tcgcgcgttc ccgtcccgtt
     2521 cgcctcgtga gtgccgggcg atctgcgggg cgatgttcgc gcacttcggc agcctgctgt
     2581 tcgagctgat caagttcagc acgcttggcc gcgaggacat gctggcgcgt gtggacatcg
     2641 agggcgacga acatctccgg caggcgtatc aacggggccg gggtgtgttc atcctcaccg
     2701 ggcacttcgg ctactgggag atacacgggc tcgtgaccgc cttgcacatg aggcccatcg
     2761 gcgtgctggc gcggccgctc gacaacccgt accttcacgc gatgctcgaa cgcatccgca
     2821 cccgcaccgg caacaccgtg atctatcgtc agggcgcgct gcggaaaatg cttcgggaac
     2881 tggggcagaa cgagggcgtg gccctcatga tcgaccagca cctgcactcc gacacggtct
     2941 ccgtcgattt cttcggccgc cgcgccgcca ccacgtcggc gctcgcggcg ctcgccattc
     3001 gtaccggcgc cgccgtgatt ccggcgttct cgctgccgct tcccaacggg cggtaccgtc
     3061 tcgtctacga gcgtccggtt ccgctgccgc ccgaggatgc ccctgatgcg gtcgccgagt
     3121 tgacgcaacg gtgtaccgac gtgctcgaga tgtacgtccg gcgggatccg cacttgtggc
     3181 tctggatgca tcgccgctgg cgcgatgggg tcagacccca tttggcccct gacgaaggag
     3241 ccgctgaaaa tggggtctag gcgcgcggtc ttcctcgatc gcgacgggac gatcatcgaa
     3301 gagcgcggct acatcgatcg gctggacatg ctgtcgctgt ttccctggac cggcgacgcc
     3361 ctgcggctcc tcaagcgggc cggactggcg accgtcgtga tcacgaacca gtcggcggtc
     3421 ggccgcggca tcatcgacga ggcgttcctc gacaccgttc accaggagat caaccggcgg
     3481 ctgcagccgt cgggcgccgc gatcgatggc tactattact gccctcactt cgcggactcg
     3541 acgatcgagc gctaccgccg tgaatgccgc tgtcgaaagc cgtcgcccgg cctcgtcgaa
     3601 caggcctgcc gcgagatgga tctcgacccg gccaggtccg tgatggtcgg agaccgctgg
     3661 atcgacgtcg cctgcgcccg cgccgctggc acgcgcgccg tgctcgtccg atcggggcac
     3721 ggacggcacg aagaggcggt gccgccgaag gacgttcgag cggatgctat tctcgacaac
     3781 ctgatggagg ccgtcgggtg gattctgcgg ggcgcggagc ggacgggtgg gtgaggtcgt
     3841 ctcgctggag cagcttctcg atcgcgttcg cggtcttcga cagaccggcc tgacgatcgc
     3901 cctggccgac ggcactttcg atctgctcac ggccggccac gtgcgatatc tcgaatcgac
     3961 cgcacaggag gccgatctgc tcgtcgtcgg catcgcaagc gacgcgacgg tgaccgatct
     4021 cgagggagaa ggccgcccga ttctcgcggc cgagcatcga gccgcgctcg tcgccgcgct
     4081 ccgctgcgtg gacttcgtcg tcgtgtactc ggagccgacc gcaggtccgc tcgccgaggc
     4141 cctgcttccg gacgtgcatc ggacaggcgg ctcccgcacc gaatcgtgac cgagcgcccc
     4201 cgacggttcc tcgtcgtgcg cctgggatcg atgggtgacg tcatccacgc gatccccgcg
     4261 gtcgcctcac tccgcgcccg gcacgctgcc gcgcggatcg attggctcgt cgatccgcga
     4321 tacgcggcgg taccgcagat ggtccgcggc gtggaccagg cgattccgat cgacacccgc
     4381 ggatccgcga tcgacctgct tcgtacgatt cggcggctgc gccaggccgg atacgacgcg
     4441 gcggtcgatc tgcaggggtt gatcaagtcg gccgtgctgg cgcgcgcagc cggcgcccgg
     4501 cggacgctcg ggtttccgcg agagcgcctg cgcgagccga tcgcgcgcgt cttctacacc
     4561 gagacacacg accccggccg cgacgcgcac gtcatctcca agaacctcgg cctgatgcgc
     4621 gcgctcggcg tcgaagatgt gcaggtgacg tttccgttga acgtgcccag gacgccggcg
     4681 ggcgacaacg ccgcggcggc gttcggcccc gatggctacg ccgtgattca cccgggggcc
     4741 gcatggccga acaagcgctg gccggccgag agattcggcg ccgtcgccgc cgggattcgc
     4801 gcgcggattg gggtgcgctc gctcgtgctg tggggcccgg gggaggagag cctggcatcg
     4861 ctcgtggcgg ccgcctcgaa gggatgcgcc gacgtctcgc cccgcacgag catcgtggat
     4921 ctgttcgcca tcgtcagggg cgcgaagctg atgatcgccg gcgatacggg accgctgcac
     4981 gtcgcggcct ccgtcggcac gccgatcgtg gggctgttcg gaccgacgct cgccgagcgc
     5041 agcggcccgt acagccgggc cgacatcgcg ctctcgcgca cgagccgatg cagctgccgc
     5101 gacgagcggc ggtgccgcct gccgcagccc tgcatcgacg acatcggcgt cgaggaagtg
     5161 gtcgcggcag tggaacgccg gctggcccaa tggccaaatc cacaatgatt gatttccttg
     5221 cacgaaaacg cgtgacgctc ggcttcgtcg tggcgctcgc ggtggcgttc ttcgcgcggc
     5281 ccacgtggga gagttggcgc attggcctcc tcgtggccgg cgtcggagag ggcctgcgca
     5341 tctgggcggc aggccatctc gagaaggggc gcgaggtgac ccgctcgggt ccgtaccgat
     5401 ggacggcgca tccgctgtat gtcgggtcgt
//
LOCUS       DBNGSH010000756         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4668948, whole genome shotgun sequence.
ACCESSION   DBNGSH010000756 DBNGSH010000000
VERSION     DBNGSH010000756.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4668948"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..2088
                     /gene="nuoL"
                     /locus_tag="AC16CY_17430"
     CDS             70..2088
                     /gene="nuoL"
                     /locus_tag="AC16CY_17430"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3355636.1"
                     /translation="MLALIPLFPLLGFVVNAAIGRRLSKSVSGGLASLVMLASFAVSV
                     VSVWQLAGMEPESRAIEQTLYTWISSGALDISASFRLDPLSAVMILVITGIGSLIHIY
                     STAYMHDEVDGEFARYFSYLNLFAAFMLVLVLGSNFPVMFVGWEGVGLCSYLLIGFWF
                     KKKSASDAGKKAFVVNRIGDYAFILGMLALFARFGTQDFQGIASAVAGLPAETGFGVL
                     SLATLLLFVGATGKSAQIPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIGRNAVLF
                     SHAPETMMIVAVIGALTALMAGTIGLVQNDIKRVLAYSTVSQLGLMFLAMGVGAFGAG
                     IFHLYTHAFFKALLFLGSGAVIHALHGEQDIRNMGGLWKELPITFWTFLIGAVAIAGV
                     PPLAGFFSKDEILFETYVGGHTVLWVIGVATSLLTATYMFRLVFLTFGGARHRPESGT
                     HQPASDHSHAHGQGSGSHLHDAPPAMALALLVLAIGSVAAGWVGCPHALGGHNQMHAW
                     LEPAFEARAELAAVAAAAAEGAPTQGEAAADAAAHESLELTLMVFSSVVAGIGILLAY
                     VMWVRRRDVPEAWARRFGGVYQTLLNKYYVDEAYDATIVQPIEAASREGLWRWFDVKV
                     IDGAVNGTGAIVDGGASLLRRLQTGSVRAYAGSLFIGVVMILGYYLWR"
     gene            2093..>2540
                     /locus_tag="AC16CY_17435"
     CDS             2093..>2540
                     /locus_tag="AC16CY_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HIR3355637.1"
                     /translation="MLTLSWAVPLAGALLLLLVGNADGRRNVLVRWLALAVSLATFGV
                     TLALWIGFDSSSAEFQFVERAPWIPAFGIDYYIGIDGISLMLVVLTGFLTPIALLSSW
                     ESIDRKVKEFSIFMLALEAAMIGVFVSLDLFLFYVFWDAMLVPMYFL"
BASE COUNT          338 a          854 c          847 g          501 t
ORIGIN      
        1 tcatcgccct cttccggcag cgcgagtcgc tcaacccgga cgccttcacg tcgctgaaat
       61 ggtgactccg tgctggcgct gatcccgctg ttcccactcc tgggcttcgt cgtcaacgcg
      121 gcgatcggcc ggcggctgtc caagagcgta tccggcggcc tggcgtcgct cgtcatgctc
      181 gcctcgttcg ccgtgtcggt ggtgagcgtc tggcagctcg cgggcatgga gccggagtcg
      241 agggcaatcg aacagacgct ctacacctgg atctcctcgg gcgctctcga catctcggcg
      301 tcgttccggc tcgatccgtt gtcggcggtg atgatcctgg tcatcaccgg gatcggctcg
      361 ctgatccaca tctactcgac ggcgtacatg cacgacgagg tggacggcga gttcgcgcgg
      421 tacttctcct acctgaacct gttcgccgcc ttcatgctgg tgctcgtcct cgggtcgaac
      481 ttcccggtga tgttcgtcgg ctgggagggc gtcggcctct gctcgtacct gctcatcggc
      541 ttctggttca agaagaagtc ggcctccgac gccggcaaga aggcgttcgt cgtcaaccgc
      601 atcggcgact acgcattcat cctcggaatg ctcgcgctgt tcgcccgctt cgggacgcag
      661 gacttccagg gcatcgcctc cgccgttgcg ggcctgccgg cggagaccgg cttcggcgtg
      721 ctctcgctgg cgacgctgct tctctttgtc ggggcgaccg ggaagtcggc gcagatcccg
      781 ctctacacct ggctgcccga cgccatggag ggcccgacgc cggtgtcggc gctcatccac
      841 gccgcgacga tggtgacggc aggcgtgtac atgatcgggc gcaacgccgt gcttttcagc
      901 cacgccccgg aaacgatgat gatcgtggcg gtcatcgggg ctctcaccgc gctgatggcc
      961 gggacgatcg ggctcgtgca gaacgacatc aagcgcgtgc tcgcctactc gaccgtctcg
     1021 cagctcggcc tcatgttcct ggcgatgggc gtcggcgcgt tcggcgcggg catcttccat
     1081 ctctacaccc acgccttctt caaggcgctg ctcttcctcg ggtccggcgc cgtgatccac
     1141 gcgctgcacg gcgagcagga catccggaac atgggcgggc tgtggaagga gctgccgatc
     1201 acgttctgga cgttcctcat cggcgcggtc gcgatcgccg gcgtgccgcc gctggccggc
     1261 ttcttcagca aggacgagat cctgttcgag acctacgtcg ggggccacac ggtgctgtgg
     1321 gtgatcggcg tggccacgtc gctgctcacg gcgacctaca tgttccgcct cgtcttcctc
     1381 acgttcggcg gagcgcggca ccggcctgaa tcgggcacgc accaacctgc gtcggaccac
     1441 tcgcacgcgc acggacaggg atcgggttcg cacctgcatg acgcccctcc cgcaatggcg
     1501 ctcgccctgc tggtccttgc aatcgggtcc gtcgcggccg ggtgggtcgg gtgtccgcac
     1561 gcgctgggag ggcacaacca gatgcacgcg tggctcgagc cggcgttcga ggcgcgggcc
     1621 gaattagcgg cggtggcggc ggcggccgcg gaaggagcgc cgacgcaagg agaggccgcc
     1681 gcggacgctg cggcgcacga aagcctcgaa ctcacgctca tggtgttctc gagcgtcgtg
     1741 gccggcatcg gcatcctgct cgcctacgtc atgtgggtga ggcggcgcga cgtgcccgag
     1801 gcgtgggccc gccggtttgg cggcgtctac cagacgctgc tgaacaagta ctacgtggac
     1861 gaagcgtacg acgcgacgat cgtgcagccg atcgaggcag cctcgcgcga ggggctgtgg
     1921 cgatggttcg acgtcaaggt gatcgacggc gccgtcaacg gcacgggcgc catcgtggac
     1981 ggcggcgcgt cgctgcttcg gcgcctgcag accggttcgg tccgcgcgta tgcgggctcg
     2041 ctcttcatcg gggtcgtgat gatcctcggc tattacctgt ggcggtagac gaatgctgac
     2101 cctttcctgg gccgtgccgc ttgcgggcgc gctgcttctt ctcctcgtcg gcaacgccga
     2161 cgggcgtcgc aacgtgctcg tccggtggct ggcgctcgcc gtgtcgctcg cgaccttcgg
     2221 cgtcacgctc gcactctgga tcggattcga ctcgtcctcg gccgagttcc agttcgtcga
     2281 gcgcgcgccg tggattccgg cgttcggcat cgactactac atcggcatcg acggcatcag
     2341 cctgatgctc gtcgtgctga ccggcttcct cacgccgatc gcgctgctgt cgtcgtggga
     2401 gtcgatcgac cggaaggtca aggagttctc gatcttcatg ctggccctcg aggccgcgat
     2461 gatcggcgtc ttcgtctccc tcgacctgtt cctcttctac gtcttctggg acgcgatgct
     2521 cgtcccgatg tacttcctca
//
LOCUS       DBNGSH010000757         3599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3988691, whole genome shotgun sequence.
ACCESSION   DBNGSH010000757 DBNGSH010000000
VERSION     DBNGSH010000757.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3988691"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..1315)
                     /locus_tag="AC16CY_17440"
     CDS             complement(362..1315)
                     /locus_tag="AC16CY_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR3355638.1"
                     /translation="MTSSIRYEVLFHRHPGNPILTAADWPYPAHTVFNAGATRLSDGT
                     TLLLCRVEDRRGHSHLCAARSTNGVDGWRIDPEPTLWPDPERHPEELWGIEDPRITFV
                     DELGKYVVAYTAFSKGGPGVALALTTDFRTFERYGLVMQPDDKDAALLPRRINGRFAL
                     LHRPWTDPCADIWISFSPDLRNWGGHRQVLPARRGSWWDANRIGLSPPLIETSRGWLM
                     LYHGVRRSAAGCLYRLGAALFDLETAQRCLLRGDSWIFGPETAYEREGDVGYVTFPCG
                     YTLDADGDGINLYYGAADTSVALATGSIGQILGWLDRHGTE"
     gene            complement(1312..>3599)
                     /locus_tag="AC16CY_17445"
     CDS             complement(1312..>3599)
                     /locus_tag="AC16CY_17445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3355639.1"
                     /translation="GADYIRRFATLGNHLPRHCGIATFTTHLTDALAEQLPEADGFVV
                     AMNDAGRHHAYPSRVRFEIGEGDLSSYRRAADFLNVNQVDVLSVQHEYGIFGGKAGAH
                     LLELLRGLRMPIVTTLHTILSEPNPSQRAVLEELARLSERLIVMSESGAELLKRVHGI
                     PADQIDLIPHGIPHVPVDSGSKERLGLQGKTVILTFGLLSPDKGIEYVVDALPEILAA
                     HPDTVYVVLGATHPHVVEREGEAYRLMLESRGRHLGVGGSMIFHNRFVSQAELTEFLS
                     AADIYITPYLNAEQITSGTLAYAVGAGKAVISTPYIYARELLADGRGVLVPWRDSSAI
                     AREVVGLGHDEVRYRTMCARAAAHGREMTWPAVARQYVQSFSRALTSESRRRRTSFQA
                     QTLASRPAGWPEIDLRHVRTLTDDTGILQHAVYSVPRYEDGYCLDDNARALLLTTLLE
                     EAGTEDPAAVRALAARYLGFVSYAFDRSSGRLRNFLSYGRQWLEPHGSEDSHGRALWA
                     LGAVVGRAGDPGRHSLAFDLFHAALPAVAAFTSPRAWAYVLLGIEEYLRAFQGDSSVE
                     SLREDLTTRLFGLWQRSSGRDWSWFEDSVTYCNARLSQALIVSGARMHRTDVVDGGLR
                     SLDWLVSMQLSPEGDFAPIGCNGFLARGGAPAAFDQQPVEACTVTSACLTAYAVSGDA
                     RWMDAGVRAFDWFLGQNALQQWLYDPSTGGCRDGLHADRANRNQGAEATLSFLLALCE
                     MRAAYRTESQPAPTLALQLTT"
BASE COUNT          677 a         1190 c         1132 g          600 t
ORIGIN      
        1 ctgcgcgccc gcctatctga aagtggaagg cgaacgtgcc tgagatgctg cacggctcgc
       61 gatcgtccat gtttcttcgc actctcggca gcgccacaag gtcttgactg tgatggtttt
      121 ggcgattgtg tcgacgcgtg tgccctggca gaacgggcac gcagtcggac gggtgaccgc
      181 ctcgctgaat cgcgattgag acatcgttac tgtgttccct tcgggtgcac gcgcggcaca
      241 gatcgatgat gcggcctgta cgcaaccgcc tcagtagtca tcggtacatc ttgccttcat
      301 caggacgtcc aaactgttca ctactgcaca cggaacgcgg actgccgtgg agcggccgag
      361 gctactccgt gccgtgtcgg tcgagccagc caaggatttg accgatgctc cctgtcgcga
      421 gagcgacgct ggtatcggcc gcaccgtagt acaggttgat gccgtcgccg tccgcatcga
      481 gagtgtaccc gcacgggaac gtgacgtagc cgacgtcgcc ttcgcgctcg taggccgtct
      541 ccggcccgaa gatccacgag tcaccgcgca acagacaccg ttgcgcggtc tccagatcga
      601 acagcgccgc cccgagccgg tacaagcaac cggctgccga tcgccggacg ccgtgataca
      661 gcatgagcca tccacgcgat gtctcgatca gcggcggtga caaaccaatg cggttggcgt
      721 cccaccacga tcctcgccga gccggcagca cctgtctgtg cccaccccag ttacgcaggt
      781 caggagagaa ggatatccag atatccgcac acggatcggt ccaggggcgg tggaggagag
      841 cgaaacgccc attgatgcgg cgcggcaaca gcgccgcgtc cttgtcgtcg ggctgcatca
      901 ccaacccgta ccgctcgaac gtccggaagt cggttgtcaa cgccagcgca actcccgggc
      961 ctcccttgct gaacgccgta taggccacga cgtacttccc gagctcatcg acgaaggtga
     1021 tgcgtggatc ttcgatcccc cacaattcct cgggatgccg ttcgggatcg ggccacaacg
     1081 tgggctccgg atcgatcctc catccatcga caccattggt ggaccgtgcg gcgcacagat
     1141 gcgagtggcc tcgccgatcc tcgaccctgc agagcaggag cgtggtgcca tccgaaagac
     1201 gcgtcgctcc cgcgttgaac accgtatgcg ccgggtatgg ccagtcggcg gccgtcagaa
     1261 tcggattccc cggatgccgg tggaacagca cttcgtagcg gatggatgac gtcatgtcgt
     1321 gagctgcaat gcgagcgtcg gcgcgggctg gctctcggtc cgataggctg cacgcatctc
     1381 gcacagtgcc aacaggaacg agagcgtcgc ctccgcgccc tggttgcgat tggcgcggtc
     1441 tgcatgaagc ccgtcgcgac agccgccggt cgacgggtcg tagagccatt gctggagcgc
     1501 gttctggccg aggaaccagt cgaaggcgcg aacgccggca tccatccacc gggcgtcgcc
     1561 gctgacagcg taagccgtga gacaggcaga ggtgaccgta caggcttcga ccggctgctg
     1621 gtcgaatgcc gctggcgcgc cgcctcgcgc aaggaagccg ttgcatccga tcggagcgaa
     1681 atccccctcg ggagacaact gcatcgacac caaccagtcc agagaacgca ggccgccatc
     1741 aacgacgtcg gtccgatgca tgcgtgcgcc agagacgatg agcgcctgtg acagccgcgc
     1801 attgcagtac gtgacagagt cctcgaacca cgaccagtca cgtccactcg atcgttgcca
     1861 gagaccgaac aggcgtgtcg tgagatcctc gcgcagcgat tcgacagaac tatcgccctg
     1921 aaacgcgcga aggtattcct cgatgccgag gagcacgtac gcccaggctc tggggctggt
     1981 aaacgccgcc acggccggca gtgcggcatg aaacaggtcg aaggcgaggc tgtggcggcc
     2041 cggatcgccc gcccggccca cgaccgcgcc gagcgcccag agtgccctgc cgtggctgtc
     2101 ttccgacccg tgcggctcga gccattgcct tccgtacgag agaaagttcc ggaggcgtcc
     2161 cgacgatcga tcgaacgcgt aactcacgaa tcccaggtaa cgcgcggcca gcgcacgcac
     2221 cgccgcggga tcctcggtac cggcctcctc gagcagcgtc gtcaaaagca gggcgcgcgc
     2281 gttgtcgtcg aggcagtagc catcctcgta ccgcggaacg ctgtacaccg catgctggag
     2341 gatcccggtg tcgtcggtca gcgtccgaac gtgcctgagg tcgatctcgg gccagccggc
     2401 cggacggctg gcgagcgtct gcgcctggaa cgacgtgcgg cggcggcgcg actcgctcgt
     2461 caaggcgcgc gagaacgatt ggacgtactg acgcgccacc gccggccacg tcatctcccg
     2521 gccgtgcgcg gccgcacgag cgcacatcgt cctgtagcga acctcgtcgt gacccagccc
     2581 gacgacctcg cgcgcgatgg cactcgaatc cctccagggc acgagcaccc cccgcccgtc
     2641 cgcgagcagc tcgcgcgcgt agatgtacgg tgttgagatg accgctttgc ctgcccccac
     2701 ggcgtacgcg agcgtgcctg atgtaatctg ctccgcgttg aggtacggcg tgatgtagat
     2761 gtcggccgcc gacaggaatt ccgtcagttc ggcctggctc acgaagcggt tgtgaaagat
     2821 catgcttccg ccgacaccaa ggtgcctgcc ccgcgactcg agcatcagcc gatacgcctc
     2881 gccttcacgc tcgacgacat gaggatgcgt ggcgccgagc acgacgtaca cggtatcggg
     2941 atgagccgcg agaatctcgg ggagcgcatc aacgacgtat tcgataccct tgtccgggga
     3001 aagcagcccg aaggtcagaa tgacggtctt cccctgcaag ccgaggcgtt ctttgcttcc
     3061 gctatcgacg ggaacgtgag gaatgccatg tggaatcaga tcgatctgat ccgcgggaat
     3121 gccatgcacc cgcttcagca actcggcgcc tgactcgctc atcacaatca gtcgctccga
     3181 caggcgagcc agctcctcga gcactgcgcg ctgagacgga ttcggttcgg acaggatcgt
     3241 gtgcagcgtc gtgacgatgg gcatccgcag gccccgcaac aactcgagga ggtgcgcacc
     3301 cgccttgccg ccgaaaatcc cgtactcgtg ctggaccgac agcacgtcca cctggttgac
     3361 gttcagaaag tcggccgcgc ggcgatacga gctcaggtcc ccttccccga tttcgaatcg
     3421 gacgcgcgag ggataggcat gatgacggcc ggcgtcgttc atggccacga cgaatccgtc
     3481 ggcctcgggc agctgttcgg ccaacgcatc cgtcagatgc gtcgtgaacg tcgcgatgcc
     3541 gcaatggcgc ggcaagtgat tcccgagcgt cgcaaatcgc ctgatgtaat ccgcacccg
//
LOCUS       DBNGSH010000758        10359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_593337, whole genome shotgun sequence.
ACCESSION   DBNGSH010000758 DBNGSH010000000
VERSION     DBNGSH010000758.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10359
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_593337"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="AC16CY_17450"
     CDS             complement(<1..647)
                     /locus_tag="AC16CY_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3355640.1"
                     /translation="MTQALPLDGITVVSIEQAIAGPFATRQLADLGARVIKVERPGVG
                     DFARSYDTTVRGLASHFVWTNRSKQSLTLDLKTPEGAGVLERLIASADVCLHNLAPGA
                     MARLGFGSAALRERYPRLVICEISGYGTSGPYRDKKAYDLLVQSEAGLVSITGTPETP
                     SKVGISVADIAAGMYAFSSILAALLRRATTGAGAVLDISMFDALAEWMGYAAYYT"
     gene            complement(647..1576)
                     /locus_tag="AC16CY_17455"
     CDS             complement(647..1576)
                     /locus_tag="AC16CY_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR3355641.1"
                     /translation="MTPDPISRLMRSMLFVPASKPAMIAKAAASAADAVCIDLEDSVT
                     PDEKPAARASVVRALSDVDFGHRVRMVRINALEGPFAYRDLVEIVEAAGDRLDLIMVP
                     KVGSARDVVFVDMLLSQIEAHIRRRTPIGIEAQIETAKGFLDARQIAESSPRLEALIF
                     GSGDYAASMRMPSSAIGERDAHDEIYPGHRWHAVMHMIVASARANGLRCMDGPYAGYT
                     DAAGLEHASRIAKSLGFDGKQCIHPAQLAIVNAAFSPSEDEVAKAAAIVQAYDQAAAA
                     GQGAATHDGRMIDAANVRMARTTLERQRLIAKR"
     gene            complement(1637..2806)
                     /locus_tag="AC16CY_17460"
     CDS             complement(1637..2806)
                     /locus_tag="AC16CY_17460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3355642.1"
                     /translation="MTEINPRASEFAEYRSAVRQLCGRFGSDYWREMDERAAYPEGFV
                     QALADAGWLAALIPEEYGGGGLGVTEASVILEEVNRSGGNSGACHAQMYIMGALLRHG
                     SAEQKRAYLPDIAAGKLRLQSFAVTEPTSGTDTTKIRTFAARRGDRYVVNGQKVWISR
                     IQHSDLMLLLARTTPLQEVKRKTEGLSLFLVDLRGATGRGMTVRPIRNMVNHETNEVF
                     FEDFEVPASQLVGQEGQGFRYVLDGMNAERILIAAECIGDGYWFIDRARHYGSERVVF
                     DRPIARNQGIQFPIARAYANVRAADLMRYEAARLFDAGAAAGAEANLAKLLAADASWE
                     AANVCLQTLGGFGFAAEYDVERKFRETRLYQVAPISTNLILSYIGEHVLGMPRSF"
     gene            complement(2814..4268)
                     /locus_tag="AC16CY_17465"
     CDS             complement(2814..4268)
                     /locus_tag="AC16CY_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HIR3355643.1"
                     /translation="MPPPRAAAASRPAIGALRVVVPLAIGAAIAITPVPEGLPRDAWL
                     YFALFTTVIAALITEPIPPAIIGMGGVIAAAVLGLVRDGPAPSAQWALSGFANSTVWL
                     IFAGYMFTLGYTQTGLGRRIALHLVRLLGKRTLGLGYAVALADLALAPFTASATARSA
                     GTVFPVVRQIPELYASRPDDGTARRLGAYVLYSALATSFVTSSMFVTALAPNTLALTV
                     MNQVAGVSVSWLDWFVGFAPVGITLLVVVPFLLYKIYPPEIRSAPEAPRWAAEQLRAM
                     GTITRRELTLLVLVCSALALWIGAIHRIEPAVTAILVVLLMVVSGVVTWNDVIGHAPA
                     WNVLIWFATLVTLAGGLAETKFLEWLAQSLGPTLEALPLSAAILAVVGVYFFLHYFFA
                     SITAHAASMLPVFLGMAVMIPGLSPTQWALLLSYPLGLMGVLTTYTAGHNPIYYGSGY
                     ISRQAFWGLGLVLGVFYFLTYLVIAVPWLSYLGV"
     gene            complement(4359..4595)
                     /locus_tag="AC16CY_17470"
     CDS             complement(4359..4595)
                     /locus_tag="AC16CY_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355644.1"
                     /translation="MTTKLDAWPVRVAAIELRQYTEFAFGQLERRMVDGFTRLEKLRA
                     VDSARLERLERKLDQFIDSQSRHRPRSKARRRKQ"
     gene            complement(4621..5553)
                     /locus_tag="AC16CY_17475"
     CDS             complement(4621..5553)
                     /locus_tag="AC16CY_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355645.1"
                     /translation="MTAAGQAPRTPWGDPDLQGAYTNSNESLIPMERPDALAGRSLGD
                     ISPAELARLNDRLNEARVEADRQRWELRSPLHWFENHAPKNSRAWLVVDPPDGKIPAQ
                     TDAARARAAARAQARRGRGEADSYEDRSLYDRCISRGLPGSMMPAIYGNSYEIVQGPG
                     FVAIQYEMVNETRVIPLDPIGELGVDGERSRTIDGRAHVGRAIRSYMGDARGRFEGDT
                     LVVETTNFTDKIPYRGSSENLRLVERFKWVGPSTVEWSVTFDDPATWIRPWTFAMNLT
                     RTSERPFEYACHEGNYAMRNILSIARQEEAARGR"
     gene            complement(5765..7147)
                     /locus_tag="AC16CY_17480"
     CDS             complement(5765..7147)
                     /locus_tag="AC16CY_17480"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3355646.1"
                     /translation="MTLARQLADWVSGLRFDDLPPDVVEATKLRLLDVFGLALAGGGT
                     RLGRSIRAGAIAVSPPGPCRIVGSGERVGLTTAAFANASLAQALEFDDTHNESIVHMS
                     SPSASAALALSETRRVSGRDLILAIALSNEIACRAGVVASGQFHRRGFHPTGLFSPFG
                     VAYAAGKILGLQADALAYAAGTCGSFASGVLECWVDGTDTKFLHSGWAAQSGLSAALF
                     AESGVTGPPKIFEGRFGFFATHIQDGTVAMRFDRMVERLGTHWESRNSSFKPYPAAHV
                     LHPYVSALLRLREKEKIRPGDVERIDCPVAEFNVSIVCEPVEEKRAPATQAHGRVCLQ
                     YTLAEALYRGALGRNAYEDEARLNPEILALARRVEYHVDPAFPGPGRFKGIVRVALKD
                     GRVLTEVEEYNRGSAENPMSEAELRAKFDDNAGGALPASARDRLASEIERTENLSDAS
                     TLVDLSVGRP"
     gene            complement(7150..8442)
                     /locus_tag="AC16CY_17485"
     CDS             complement(7150..8442)
                     /locus_tag="AC16CY_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3355647.1"
                     /translation="MSPTTIDHVTKERERRVFAKIAWRLMPVLTVSYILNYLDRTNVS
                     FAALTMNEAIGLTATQFGIGAGIFFLGYCLLEVPSNLVLYRVGARVWISRIMISWGLV
                     SAAMSLVVGPTSFYFMRALLGAAEAGFFPGVAFYLATWFPAEYRTRIVAWFMVAIPIS
                     AVIGGPVSGLLLSMDGTWGLAGWQWLFIAEGLPAVLVGLSLLWLMFDSPENTAWLTEE
                     EKRIVRNRLQSERRPKEVKRLSLAVQDVRVLILACIQFGFLVGSYGIGIFMPQILDTG
                     RLTDVQIGFVTSGCYLVASVGMIVWAGYVDRGHSKVVNLALACGLSAIGFLGAIAFAD
                     NLWLAVPWMAVAVTGINGARAIFWTIPPRFLTGVAAAGGLAFINSIGTAGGFVGPTIM
                     GWLTDLTGSYSAGLFALTGFLLVSATLAWTLRRFAPGE"
     gene            complement(8463..8873)
                     /locus_tag="AC16CY_17490"
     CDS             complement(8463..8873)
                     /locus_tag="AC16CY_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355648.1"
                     /translation="MSEFSGRRILAVVTSVVVVAAVGTGIVLMGPPSEERLRRLDERR
                     VEDLQQVSFSVNLYNNRHQRLPESLVELLAEPGMTFRPGDPVTEEFYGYRVLEPAKYE
                     LCAVFDRASTERRASVWSHGGGRHCFVLKADEAR"
     gene            complement(8895..9593)
                     /locus_tag="AC16CY_17495"
     CDS             complement(8895..9593)
                     /locus_tag="AC16CY_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HIR3355649.1"
                     /translation="MATGDHLLDFVKDALGRGVPRPQIEEALRKAGWTADQIRGALTA
                     FAEVDFPIPVPRAKPYLSAREAFMYLVLFGTLYSSVYSLGSLAFDFINIAFPDPAAGS
                     SFRETEEYVRQSIRWSVSMLIVAFPVFMYTSLLTGRAISLDPTKRASKVRRWLTYWTL
                     FIAACALIGDVTTLVYNLLGGELTVRFMLKVIVVALIAGTAFTYYLRDLRQDEEETGL
                     KGAGGRGQGAGVRG"
     gene            complement(9603..>10359)
                     /locus_tag="AC16CY_17500"
     CDS             complement(9603..>10359)
                     /locus_tag="AC16CY_17500"
                     /inference="COORDINATES: protein motif:HMM:NF019533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HIR3355650.1"
                     /translation="LPIVGIWPLAEGETAFGADADLPIVLPAAGTGRAGVFRRQGSTI
                     TLVPAAGAALRTADGKPIDQATEVSEEVWLGSLRMSIAGAPDGRLWVSASDEQHPAVK
                     NPPTVEAYPLDPRWRIAARFDAFESPKPVRVPDVRGGTMDFTAMGQLVFRVNGQEMRL
                     TALGEPGSEQFFVMFKDPTNQTTTYGGYRILNPAVVATGEWTVVDFNYASNPPCAYSK
                     YTVCPLPPPENRLPVAVEAGLKRLPSAQGYSPS"
BASE COUNT         1808 a         3563 c         3396 g         1592 t
ORIGIN      
        1 tcggtgtagt acgccgcgta ccccatccac tcggcgaggg cgtcgaacat cgagatgtcg
       61 agcacggcgc ccgcgcccgt ggtcgcccgg cgcaggagcg cggcgaggat cgacgagaag
      121 gcgtacatgc cggccgcgat gtcggcgacg gaaatgccga ccttcgacgg cgtctcgggc
      181 gtcccggtga tggagacgag gccggcctcg ctctggacca gcaggtcgta cgctttcttg
      241 tcgcgatacg gaccggacgt gccgtacccg gaaatctcgc agatcaccag gcgcgggtat
      301 cgctcccgca gcgcggccga gccgaatccc aggcgtgcca tcgctcccgg agcgaggttg
      361 tgaaggcaga cgtcggcgct ggcgatgagc cgttcgagga cgccggcgcc ttccggcgtc
      421 ttgagatcga gcgtgagcga ctgcttcgag cgattcgtcc acacgaagtg gctcgccagt
      481 cctctcaccg tggtgtcgta cgagcgggcg aagtcgccga cgccgggacg ctcgaccttg
      541 atcacccgcg caccgaggtc tgccagctgg cgcgtggcaa acggcccggc gatcgcctgc
      601 tcgatcgaga cgacggtgat gccgtcgagt ggcagcgcct gcgtcatcag cgcttcgcaa
      661 tgagccgttg gcgttccagc gtcgtacgcg ccatgcggac gttggcggcg tcgatcatcc
      721 gcccgtcgtg cgtggccgcg ccctggccgg cggccgccgc ctgatcgtag gcctgcacga
      781 tggccgccgc cttcgcgacc tcgtcctcag acggggagaa tgccgcgttg acaatggcca
      841 gctgcgccgg gtggatgcac tgcttgccgt cgaagccgag cgacttcgcg atgcgggagg
      901 cgtgctcgag accggcggca tcggtgtagc ccgcgtaggg cccgtccatg catcgcaggc
      961 cgttggcgcg cgccgaggcg acgatcatgt gcatcacggc gtgccagcgg tgcccgggat
     1021 agatctcgtc gtgcgcgtcg cgctcgccga tggccgagga cggcattcgc atggaggcgg
     1081 cgtaatcgcc cgacccgaaa atcagcgcct cgaggcgcgg cgacgattcg gcgatctgcc
     1141 gggcgtcgag aaagcctttg gccgtttcga tctgggcctc gatcccgatg ggggtacgac
     1201 gccggatgtg cgcctcgatc tgggacaaca gcatgtccac gaacacgacg tcgcgggccg
     1261 acccgacttt cggcaccatg atgaggtcca ggcgatcgcc ggcggcttcg acgatctcga
     1321 cgaggtcccg gtaggcgaag ggtccctcga gcgcattgat ccgcaccatt cggacgcggt
     1381 ggccgaagtc cacgtccgac agcgcgcgaa cgacgctggc gcgtgcggcc ggtttctcgt
     1441 caggcgtgac cgagtcttcg aggtcgatgc agacggcgtc ggcggcgctg gcggcggcct
     1501 tcgcgatcat cgccggcttc gacgccggca cgaacaacat gctgcgcatc agtcgggaaa
     1561 tggggtcggg agtcattttc tgaggacact ttccactgcc cttcagaaaa tgactcccga
     1621 ccccatttca gatttgtcag aacgatctcg gcatgccgag cacgtgctct ccgatatacg
     1681 agaggatcag gttcgtcgag attggcgcca cctggtacag ccgcgtctcc ctgaacttgc
     1741 gctccacatc gtactcggcg gcgaagccga acccgcccag tgtctgcagg cagacgttgg
     1801 ctgcctccca gctcgcgtcg gccgcaagga gcttggccag gttggcctcg gccccggcgg
     1861 cggcgccggc gtcgaacagg cgcgcggcct cgtaccgcat cagatccgcc gccctgacgt
     1921 tggcgtacgc ccgggcaatc ggaaactgaa tgccctggtt ccgcgcgatc gggcgatcga
     1981 agacgacccg ctcgcttccg tagtgcctcg cgcggtcgat gaaccagtac ccgtcgccga
     2041 tgcattcggc cgcaatgagg atccgctcgg cgttcatccc gtcgaggacg taccggaatc
     2101 cctgcccctc ctgccccacc agctgcgacg ccggcacctc gaagtcctcg aagaacactt
     2161 cgttcgtctc atggttgacc atgttccgga tcggccgcac ggtcatgccc cgcccggtgg
     2221 cgccgcgaag atcgaccagg aacagcgaca gcccttcggt cttccgcttc acctcctgaa
     2281 gaggcgtcgt ccgcgccagc agcagcatca gatcggagtg ctggattcgc gaaatccaga
     2341 ccttctgtcc gttgacgacg tagcggtcgc cccggcgggc ggcgaacgtt cgtatcttcg
     2401 tggtatcggt gccagacgtg ggctcggtga cggcaaacga ctgaagccgc agtttgccgg
     2461 cggcgatgtc gggcaggtac gcccgcttct gctcggccga cccgtgccgc aggagcgcgc
     2521 ccatgatgta catctgcgcg tggcacgcgc ccgagttgcc gcccgaccgg ttcacttcct
     2581 cgagaatgac ggacgcctcg gtcacaccca gcccgccgcc gccgtattcc tcgggaatca
     2641 gcgccgccag ccagcccgcg tcggcgagcg cctgcacgaa gccttccggg tacgccgcgc
     2701 gttcgtccat ctcgcgccag tagtcggagc cgaagcgccc gcacaactgg cggaccgcgc
     2761 tgcggtactc cgcgaattca gaggctctcg ggttgatctc ggtcacgggc acatcacact
     2821 cccaggtagg aaagccaggg aaccgcaatg acgaggtacg tgaggaagta gaacactccg
     2881 agaaccaggc cgagtcccca gaacgcctgc cggctgatat agccgcttcc gtaatagatc
     2941 ggattgtgcc ccgccgtgta ggtggtcaac acgcccatca gcccgagcgg gtacgagagc
     3001 agcagcgccc actgcgtggg cgacagcccc ggaatcatca ccgccatgcc gaggaacacg
     3061 ggaagcatgc tcgcggcatg cgccgtaatg ctggcaaaga agtagtggag aaagaagtag
     3121 acgcccacca ccgcgaggat cgccgcagag agaggcagcg cctccagcgt cgggccgagc
     3181 gactgcgcga gccactcgag aaacttcgtt tcggccaggc cgccggcgag cgtgaccaaa
     3241 gtcgcgaacc agatcaggac gttccatgcc ggggcgtgcc cgatcacgtc gttccacgtg
     3301 acgacgccac tcacgaccat caacagcacg accaggatgg ctgtgaccgc gggctcgatg
     3361 cggtggatgg caccgatcca cagggcgagc gcggagcaca ccaggacgag aagcgtgagc
     3421 tcccgtcgcg tgatcgtgcc catggcgcgc agttgttctg ccgcccatcg cggcgcctcg
     3481 ggcgcgctcc gaatctccgg cgggtagatc ttgtagagaa gaaacggtac gaccaccagc
     3541 agcgtgatgc cgaccggcgc gaagccgaca aaccagtcga gccacgacac cgagacgccc
     3601 gccacctggt tcatcaccgt cagcgccagc gtattcgggg caagagcagt cacgaacatg
     3661 ctgctcgtca cgaagctcgt cgcgagcgct gaatacagca cgtacgcccc cagcctcctc
     3721 gccgtcccgt cgtcagggcg agaggcgtac agctcgggaa tctgccgcac gacggggaac
     3781 acggttcccg cactgcgagc cgtggcagac gcagtgaagg gagcaagcgc caggtcggcg
     3841 agcgcgacgg cgtagcccag gccgagcgtg cgtttcccga gcagacgcac caggtgcagc
     3901 gcgatgcgtc tgcccagccc cgtttgtgtg tagcccagcg tgaacatgta gccggcgaag
     3961 atgagccaca ccgtggagtt ggcgaatcca ctgagcgccc actgcgccga tggcgccggc
     4021 ccgtcgcgaa ccaggccgag cacggcggcg gcgataacgc ctcccatccc gatgatcgcc
     4081 ggcgggatgg gctcggtgat gagagccgcg atgaccgtgg tgaacagggc gaaatagagc
     4141 cacgcatcac gcggcaggcc ttcgggcact ggcgtgatcg cgatagcggc gccgatcgca
     4201 agcggcacga cgacgcggag ggctccgatc gctggccgcg acgccgccgc ggcgcgaggc
     4261 ggtggcacgg tgggggcggc gccaggcacg gcgcggcagc atagcacgca gcacgggccg
     4321 ccaagcgggg tcgcgccgcg acacggacga tcgaggttct actgctttcg ccgccgggcc
     4381 ttcgaacgcg gacgatgccg cgactgactg tcgatgaact gatccagctt gcgctcgagg
     4441 cgctccagac gggcagaatc aaccgctcgt aacttctcga ggcgagtgaa gccgtcgacc
     4501 atccggcgct cgagttgccc gaaggcgaac tccgtgtatt gacgcagctc gatcgccgca
     4561 acccgcactg gccacgcatc cagtttcgtc gtcatgtccg acttcatgat cacgaagcgc
     4621 ctaccggcct cgagcggcct cctcctgcct ggcgatgctc aggatgttgc gcatcgcgta
     4681 gttgccctcg tggcaggcgt actcgaacgg acgctcgctc gtccgggtca ggttcatcgc
     4741 gaacgtccag ggtctgatcc acgtggccgg gtcgtcgaag gtgaccgacc attcgaccgt
     4801 ggacgggccg acccacttga agcgctcgac cagccgcagg ttctcgctcg atccgcggta
     4861 cgggatcttg tcggtgaagt tcgtcgtctc gacgacgagc gtgtcgccct cgaatcgccc
     4921 gcgcgcgtcg cccatgtagc tgcggatcgc gcgcccgacg tgcgcccggc cgtcgatggt
     4981 tcgacttcgc tcaccatcga ccccgagctc gccaatgggg tcgagcggaa tgactcgcgt
     5041 ctcgttgacc atctcgtact ggatggccac gaaccccggg ccctgcacga tctcgtagga
     5101 gttgccgtaa atggcgggca tcatcgagcc gggcaggccg cgcgagatgc atcggtcgta
     5161 caggctgcga tcctcgtagg aatcggcttc gccgcggccg cgccgggcct gcgcccgcgc
     5221 cgcggcgcgg gctctcgcgg catcggtctg cgccggaatc ttgccatcgg gcggatcgac
     5281 cacaagccac gcgcggctgt tcttcggggc gtggttctcg aaccagtgca gagggctgcg
     5341 cagctcccag cgctgccggt cggcctcgac gcgcgcctcg ttgagccgat cgttgagccg
     5401 cgcgagctcc gctgggctga tgtcgccgag cgagcggccg gcaagcgcgt cggggcgctc
     5461 catcggaatc aggctctcgt tgctgttcgt gtaggcgccc tgcaggtccg ggtcgcccca
     5521 gggcgtgcga ggcgcctgcc cagccgctgt cacggccgtc agcaaaaccg tcgcacctgc
     5581 aagaacgatc gactgaagaa gtcgacgagt cactgcacac ctcgctgtga actgtgaacc
     5641 gtgctccgtg aacggcctac cgccgtcgac tgcgcgttac gcgcgttaac cacagaggcg
     5701 cagaggacac agagaatcct catccactct gtgcgctctg cgtctctgtg gttggcgttc
     5761 gtcatcaggg ccgaccgacg gacagatcaa ccagcgtaga cgcgtcggag agattctccg
     5821 tccgttcgat ctccgaggca agccggtcac gggcggacgc cgggagggcg ccgccggcgt
     5881 tgtcgtcgaa cttggcgcga agctcggcct cgctcatcgg gttctcggcc gagccgcggt
     5941 tgtattcctc gacctcggta agcacgcggc cgtccttcaa cgcgacgcgc acgatcccct
     6001 tgaaacggcc ggggcctgga aacgcggggt ccacgtgata ctcgaccctc cgtgcgaggg
     6061 cgagaatctc gggattcagc cgcgcctcgt cttcgtacgc gttccggcca agcgcgcccc
     6121 gatacagcgc ctcggcgagc gtgtattgaa gacagacccg gccgtgcgcc tgcgtcgccg
     6181 gagcgcgctt ctcttcgacc ggctcgcaca cgatcgacac gttgaactct gccaccgggc
     6241 agtcgatgcg ctcgacgtcg cccggccgga tcttctcctt ctcccgcagc cggagcagcg
     6301 ccgagacata cgggtgcagt acgtgcgcag cggggtatgg cttgaacgac gagttgcggc
     6361 tctcccagtg agtgccgaga cgctcgacca tgcgatcgaa ccgcatcgcg accgtcccgt
     6421 cctggatgtg cgtcgcgaag aacccgaagc gcccttcgaa gatcttcggt gggccggtga
     6481 cgcctgattc ggcgaacagc gcggccgaga ggccgctctg cgcggcccac cccgaatgga
     6541 ggaacttggt gtcggtgccg tcaacccagc attcgagcac gcccgacgcg aagctgccgc
     6601 acgtcccggc ggcgtacgca agcgcgtccg cctggagacc gaggatcttc cccgccgcgt
     6661 aggccacgcc gaacggggag aagagaccgg tcggatgaaa gccgcggcga tggaactggc
     6721 cgctcgcgac gacgcccgcg cggcaggcga tctcgttcga gagcgcgatc gccaggatca
     6781 ggtcgcggcc cgacacccgc cgcgtttccg agagcgccag cgccgccgac gccgacgggc
     6841 tgctcatgtg gacgatcgac tcgttgtgcg tgtcgtcgaa ctcgagcgcc tgagccagcg
     6901 acgcgttggc gaacgctgcc gtcgtcaggc cgactcgctc gccgcttccc acgatgcggc
     6961 acggaccggg cggtgacacg gcgattgcgc ccgcgcggat cgagcgccca agcctcgtac
     7021 cccctcccgc cagcgcgagg ccgaacacgt ccagcagccg tagtttcgtg gcctccacga
     7081 cgtccggcgg gaggtcgtcg aatcgcaggc ccgagaccca gtcggcgagc tggcgagcga
     7141 gcgtcatcct cattccccgg gggcaaagcg cctgagcgtc cacgcgagcg tcgccgagac
     7201 gagcaggaac ccggtcaacg cgaagagacc ggccgagtac gatccagtca gatccgtcag
     7261 ccagcccatg atcgtcgggc cgacgaaccc gcccgccgtg ccgatcgagt tgatgaacgc
     7321 cagccctccc gcagcggcca cacccgtcag gaagcgcggt ggaatcgtcc agaagatcgc
     7381 gcgcgcgccg ttgatgccgg tcaccgccac cgccatccag ggcaccgcca gccacagatt
     7441 gtcggcgaag gcgatcgcgc cgaggaagcc gatggccgac agcccgcagg ccagggccag
     7501 gttcacgacc ttgctgtgcc cccgatcgac gtacccggcc cacacgatca tcccgacgct
     7561 cgcgacgagg taacacccgc tcgtgacgaa gccaatctgg acgtcggtca accggccggt
     7621 gtccagaatc tgcggcatga agatcccgat cccgtaggac ccgacgagaa acccgaactg
     7681 gatgcaggcg aggatgagca cgcggacgtc ctggacggcg agcgacagcc gcttcacttc
     7741 tttcggccgc ctctcgctct gcagccggtt gcgaacgatc cgcttctcct cctccgtcag
     7801 ccacgccgtg ttctcgggac tgtcgaacat cagccacagg aggctgaggc cgacgaggac
     7861 cgcgggcagg ccctcggcga tgaacagcca ctgccacccg gccagccccc aggtgccgtc
     7921 cattgacagc aggaggcccg acacgggacc gccaatcacg gcggagatcg gaatggccac
     7981 catgaaccag gcgacgatgc gcgtgcggta ctcggccggg aaccacgtcg ccaggtagaa
     8041 ggcgacaccc ggaaagaaac cggcctcggc cgctccgagc agcgcacgca tgaagtagaa
     8101 gctcgtgggc cccacgacca ggctcatcgc cgccgacacg agcccccacg agatcatgat
     8161 gcgcgaaatc cagacgcgcg ccccgacgcg gtagagcacg aggttgctgg gcacctccag
     8221 caggcagtag ccgaggaaga agatccctgc gccgatcccg aactgcgtcg ccgtcagccc
     8281 gatggcctcg ttcatggtca gcgcggcgaa actgacgttg gtgcgatcga gatagttgag
     8341 gatgtagctg accgtcagga cgggcatcag ccgccaggcg atcttggcga agacgcggcg
     8401 ctcccgttcc ttcgtgacgt gatcgattgt cgttggactc actcggcggc cggccccgaa
     8461 ttctatctcg cctcgtcggc cttcagcacg aagcagtgcc tgccgccgcc gtgcgaccag
     8521 acggaggcgc gcctctcagt cgacgcgcga tcgaagacgg cgcacagctc gtatttggcg
     8581 ggctcgagca cgcggtagcc gtagaactcc tccgtcaccg gatcgcctgg ccggaacgtc
     8641 attcccggct ccgccagcag ctcgacgagc gattcaggca ggcgctggtg ccggttgttg
     8701 tacagattca cggaaaacga cacctgctgc aggtcctcga cccggcgctc gtcgagccgc
     8761 cgcaaacgct cttcgctcgg agggcccatc agcacgatgc cggtgccgac agcggccacg
     8821 acaacgaccg acgtcacgac ggcgaggatg cgtctgccgg agaactcgct cacctgcccc
     8881 ctgcccccta acccctaacc cctaacccct gccccctgcc ccctgccccc tgcccccttc
     8941 agtccagtct cctcctcgtc ctgccgcagg tcgcgcaggt agtaggtgaa cgcggtgccc
     9001 gcgatgagcg ccaccacgat caccttcaac atgaaacgga cggtcagctc gcccccgagc
     9061 aggttgtaga cgagcgtcgt gacgtcgcca atcaacgcgc acgcggcgat gaacagggtc
     9121 cagtaggtca gccaccggcg gaccttcgac gcgcgtttgg tcggatcgag gctgatggcg
     9181 cggcccgtca acaacgacgt gtacatgaac accggaaacg ccacgatgag catcgagacg
     9241 gaccagcgga ttgattggcg tacgtactct tcggtctccc ggaacgacga cccggctgcc
     9301 gggtccggaa acgcgatgtt gatgaagtcg aacgccaggc ttccaaggct gtatacgctg
     9361 ctgtacagcg tgccgaagag caccaggtac atgaacgcct cgcgcgcgga cagatacggc
     9421 ttcgcgcgcg ggacgggaat cgggaagtca acctccgcga acgccgtcag cgcgccgcgt
     9481 atctgatcgg ccgtccaccc cgccttgcgg agggcctcct cgatctgcgg acgcgggacg
     9541 ccgcgcccca gcgcgtcctt gacgaaatcg agcaggtggt cgccggtcgc catctccagc
     9601 cactaggacg gcgaatagcc ctgcgccgag ggcagccgct tcaggcccgc ctccacggcc
     9661 acgggcagcc ggttctcggg cggaggcagc gggcagacgg tgtacttcga gtacgcacac
     9721 ggcgggttgg acgcgtagtt gaagtcaacg accgtccact cgccggtggc gacgaccgcc
     9781 gggttcagaa tccgatagcc tccgtacgtc gtcgtctggt tggtcggatc cttgaacatc
     9841 acgaagaact gctcgctgcc cggctcgccc agcgcggtga ggcgcatctc ctgcccgttc
     9901 acgcggaaca cgagctggcc catcgcggtg aagtccatgg tcccgccgcg cacgtccgga
     9961 acacgcacgg gcttcggcga ctcgaacgcg tcgaagcgtg cggcgatccg ccagcgcggg
    10021 tcgagcgggt acgcctcgac ggttggtggg ttcttgacag ccggatgctg ctcgtcgctc
    10081 gcgctcaccc agaggcggcc gtccggtgcg cccgcgatgg acatccgcaa cgagcccagc
    10141 cagacttctt cgctgacttc cgtcgcttga tcgatcggct ttccgtcggc ggtacgcagt
    10201 gccgcgcccg ctgccgggac gagggtgatc gtcgatccct gccggcgaaa gacgcctgcc
    10261 cgtcccgtgc cagccgcggg tagaacgatt ggcagatccg cgtccgctcc gaatgccgtc
    10321 tcgccctccg cgagcggcca gataccgacg atgggaagc
//
LOCUS       DBNGSH010000759         2345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1441659, whole genome shotgun sequence.
ACCESSION   DBNGSH010000759 DBNGSH010000000
VERSION     DBNGSH010000759.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1441659"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC16CY_17505"
     CDS             <1..335
                     /locus_tag="AC16CY_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355651.1"
                     /translation="VDQVIEEKYGRGGTYGDNSIFDRAYKDSQHGDAFLKMASQRPTP
                     QVVEYTKEICNYIYDTYGRFTAHVNAFHLPGVWLQFSHLELEFYQKYFDADLYHRQAA
                     HHEIWGDH"
     gene            complement(551..1267)
                     /locus_tag="AC16CY_17510"
     CDS             complement(551..1267)
                     /locus_tag="AC16CY_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355652.1"
                     /translation="MTGRLHAAVFVSVLAGSACSLATGIRENSGAIAKSTAAIETNTV
                     AVRDSTMGSTTLIPALKSVEQLAGPLKAVGALEPTLQRVSTLNEPLTRVAGLEPALRS
                     VSELQQPITRLVAIGPGLDSAAALGGPLTRVADMRASLDRVAELRPSLDQVSQLEPQL
                     AAVADLRDPLQQLSALRAPLEQVGSLTAPISTLTSLLNRPLVLLLIAAAGLLAWGIVT
                     FFAVRLAILSARSRGQLERA"
     gene            complement(1365..>2345)
                     /locus_tag="AC16CY_17515"
     CDS             complement(1365..>2345)
                     /locus_tag="AC16CY_17515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3355653.1"
                     /translation="AHPSPSDAPGETRQVFAAREVILAGGTFNTPQLLMLSGIGPRAV
                     LDKCGVPVRVELEGVGKNLQDRYEVAVVNRMSFPAWKVLEGATFTREDSQYQDWANYR
                     DGVYDSNGAMISVVARSGPAAFSPDLFLYSVLGRFEGYFPGYSSLPAKNPNFLTWVIL
                     KAHTNNTAGEVTLRSKDPRVPPDVNFRYFEEGNDAAGADLKAVAAGVALVRKLAAPLK
                     AEGLIAKEELPGEDVVDQSLLEFIRNNAWGHHASCTCRIGRREDGGVLSSDFKVHGVE
                     GLRVVDASVFPRIPGVFVMSAVYMIGEKAADAIAADANTSDALGPVRPFQ"
BASE COUNT          412 a          781 c          773 g          379 t
ORIGIN      
        1 cggtggacca ggtgatcgag gagaagtacg ggcggggcgg aacctacggc gacaactcga
       61 tcttcgaccg cgcgtacaag gacagccagc acggcgacgc cttcctgaag atggcgagcc
      121 agcgtccgac gccgcaggtc gtggagtaca cgaaagagat ctgcaactac atctacgaca
      181 cctacggccg ctttacggcg cacgtgaacg cctttcacct cccgggcgtg tggctgcagt
      241 tctctcacct ggagctcgag ttctaccaga agtacttcga cgccgacctc tatcaccggc
      301 aggcggcgca ccacgagatc tggggcgatc actagcgcgc tttctgtatt gatgtcgcgc
      361 gcgtcctgaa gtccttggtg cctggttcct ggtccgttct gttgtcctga gtcctttgtc
      421 cgcactggac cggagactct ggaccacgga cggactcagg accacggacg gaccaaggac
      481 gaagcacaac ggaccaggga cgacgtctac acctgatcct catctaacca gtagggccgg
      541 tcccgttttc tcaggcccgc tccagttgcc cgcgcgaccg cgcgctcagg atcgcgagtc
      601 ggacggcgaa gaacgtgacg atgccccagg cgagaaggcc ggccgcggcg atgagcaaca
      661 acacgagcgg ccgattgagc agcgacgtga gggtcgagat cggcgcggtg agcgacccga
      721 cctgttcgag cggcgcgcgc agcgcgctga gctgctgaag cgggtcgcgg agatcggcga
      781 cggccgcaag ctgcggctcg agttgcgaca cctgatcgag cgacggccgc agctccgcga
      841 cgcggtccag gctggcgcgc atgtcggcca cgcgcgtcaa cgggccgccg agcgccgccg
      901 ccgaatcgag tcccggaccg atcgccacga gccgcgtgat cggctgctgg agctcggata
      961 ccgatcgcag cgcgggttcg agccccgcca cgcgtgtcag cggttcattg agcgtcgaca
     1021 cgcgttgcag cgtgggttcc agcgccccga ccgccttcag cggaccggcg agctgctcga
     1081 cgctcttcaa cgctggaatg agcgtcgtcg agcccatggt cgagtcgcgc acggcaaccg
     1141 tgttcgtctc gatggccgcc gtgctctttg cgatggcgcc tgagttctca cggatcccgg
     1201 tggccagcga gcaggccgag cctgccagca ccgacacgaa cactgcagca tgaagtctgc
     1261 cggtcattga ggtcctccgg tccggctgaa gccggacact acgtacgcca cggtccggct
     1321 gaagccggac actacgtacg ccaccgtccg actgaagccg gacactactg gaacgggcgc
     1381 accgggccca gggcgtccga ggtgttggcg tcggcggcga tggcgtcggc tgccttctca
     1441 ccgatcatgt agaccgcgct catcacgaaa acgcccggga tccgggggaa gaccgacgcg
     1501 tccacgacgc gaaggccctc gacgccgtga accttgaagt cgctcgacag cacgccgccg
     1561 tcctcgcgcc ggccgatgcg gcaggtgcac gaggcgtggt gcccccacgc attgttgcgg
     1621 atgaactcga gcaggctctg gtcgacgacg tcctctcccg gtaattcctc cttcgcgatc
     1681 agcccttccg ccttgagggg ggcggccagc tttcgcacca gcgcgacgcc ggccgccacg
     1741 gccttgaggt cggccccggc tgcgtcgttg ccctcttcga aataccggaa attgacgtcg
     1801 ggaggcacgc ggggatcctt cgatcgcagc gtcacctccc cggccgtgtt gttggtgtgg
     1861 gccttgagga tcacccaggt caggaagttc ggattcttcg cgggaagcga cgagtagccg
     1921 gggaagtagc cctcgaatcg gccgagaacg gagtagagaa agagatcggg cgaaaacgcc
     1981 gcgggccccg accgcgccac gacggaaatc atcgcgccgt tgctgtcgta cacgccgtcg
     2041 cggtagttcg cccaatcctg gtactgggag tcctcgcggg tgaaggtcgc tccttcgagc
     2101 accttccatg cggggaagct catgcgattc acgaccgcga cctcgtagcg gtcctgaaga
     2161 ttttttccga ccccctccag ttcgacgcgg accggaacgc cgcacttgtc gagaaccgcg
     2221 cgcggcccga tgcccgacag catcagcaac tggggcgtgt tgaaggtgcc tccggccagg
     2281 atgacttcac gggcggcgaa cacctggcgc gtctcgcccg gcgcgtcgct cgggctcgga
     2341 tgcgc
//
LOCUS       DBNGSH010000760         3391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3649158, whole genome shotgun sequence.
ACCESSION   DBNGSH010000760 DBNGSH010000000
VERSION     DBNGSH010000760.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3649158"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="AC16CY_17520"
     CDS             complement(<1..603)
                     /locus_tag="AC16CY_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3355654.1"
                     /translation="MHRITPTMRSLLLGATAAAIFVTTTYGQSILGPPSEANHFIETP
                     KGWTHPRTPWGEPDIQANLNMMQAAGIPLERCASSIGRGGPCDMNKAWRTPQEYKEAL
                     DAAAGRGDRGRQLLAEGNFGAALLAGVVDLSTPQRQTTLIVDPPSGLLPALTPEGKQR
                     ALQMRSGWSLPGESPSYDGPGDFDSWDRCTTRGMPSSMMPY"
     gene            complement(734..1006)
                     /locus_tag="AC16CY_17525"
     CDS             complement(734..1006)
                     /locus_tag="AC16CY_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355655.1"
                     /translation="MMSDDLGTAASSHLASLSHAVPDSRRAERTRLRCRARLVRRQRR
                     RNHAASTIRLARGVLTAVAVGGFVALCVLYVSSLVATTVSLEGALR"
     gene            complement(1003..1590)
                     /locus_tag="AC16CY_17530"
     CDS             complement(1003..1590)
                     /locus_tag="AC16CY_17530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3355656.1"
                     /translation="MDRERELVLVNRLRSGDPDAFDEVHGVFNVRLFNFLFRLSNRRE
                     VAEDLLEETWLRLVTHAQRLQGDTRLGAWLFTVARHLHVSYCRSRLLEESHAADLIGL
                     WPGGSRRPSQSEVMEASEAQRRVATALASLPAAYREALLLVVVEGLRQSEAAAICGVT
                     GVAMRQRVSRARMRLARRLAETDGPGLASLNEVTT"
     gene            1735..2022
                     /locus_tag="AC16CY_17535"
     CDS             1735..2022
                     /locus_tag="AC16CY_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355657.1"
                     /translation="MGVVPLGMGIVYAISPSEQRLALMRPLSLAAIFGALSGTMAGVI
                     NSLVGMARTQTPVFSPVVAAGLAESLVPVFFGFGCLTVAWLCVAVGLWRKA"
     gene            2220..2717
                     /locus_tag="AC16CY_17540"
     CDS             2220..2717
                     /locus_tag="AC16CY_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355658.1"
                     /translation="MGRKALWIVGAAVGLALYAAPAHAQVSVSIGVGGPWFGAGVVVG
                     GPVYGAPYYYPYYSYPYPYYYPYPVYSYPYPVYRYRTAYPYRYPARYGRAYYGGSAVV
                     RQAQPYYRGAQGRYYNNGPGAPAQYRRGAAPAQRVGPAQRTAPAQRGNAPRGNGQAVR
                     VARQR"
     gene            complement(2834..>3391)
                     /locus_tag="AC16CY_17545"
     CDS             complement(2834..>3391)
                     /locus_tag="AC16CY_17545"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3355659.1"
                     /translation="YAELHRAHPFQVPPDFVARLRSLLGIMLMVLFVQACRAPAVPDG
                     DTGVDGRLAAADAVAVRAALDSYFASAIGRDPSRWAALYADNAVVMPPNGATVEGRSA
                     IEAWLRALPVVISEADGVALEVDGSGDFAYVRGTYALAMEAPGVPQPIRQEGKFLQIY
                     TRQEDGTWLLARDIWNANASPSGGP"
BASE COUNT          535 a         1151 c         1122 g          583 t
ORIGIN      
        1 atacggcatc atcgacgacg gcatgccgcg cgtcgtgcag cggtcccagc tgtcgaagtc
       61 gccaggcccg tcgtaggaag gcgactctcc gggcagcgac cagccgctgc gcatctggag
      121 cgcgcgctgc ttgccctccg gcgtcagggc cggcagcagt ccgctgggcg gatcgacgat
      181 gagcgtggtc tgccgctgcg gagtgctcag atcgacaacg cctgccagga gcgccgcgcc
      241 gaagttgcct tcggcgagca gttgacggcc gcgatcgccc cggccggcag cggcgtcgag
      301 cgcctccttg tattcctgtg gcgtccgcca cgccttgttc atgtcgcacg ggccgccgcg
      361 gccgatcgag ctcgcgcacc gctcgagcgg gatgccggca gcctgcatca tgttcaggtt
      421 ggcctggatg tctggctcgc cccacggcgt cctcggatgc gtccagcctt tgggcgtttc
      481 gatgaagtga ttggcctcgc tcggcgggcc gagaatggac tggccgtacg tggtagtcac
      541 gaaaatcgcg gctgccgttg cgccaagcag caggctgcgc atcgtcggcg tgatccggtg
      601 catggtcgct cctggggcgc ggagcggcgc ccgtcgtagt gtctcacgac tccagcgtac
      661 acggcccgcc gtcgtgatca ttcgagatct atcggcacca gaacgactcg acttgaatgg
      721 ggcagtgccc gaatcagcgt agcgctccct cgaggctgac cgtcgtcgcc acgagcgagc
      781 tgacgtagag gacgcagagc gcgacgaagc cgccgacggc cactgccgtc agcacgcctc
      841 gcgcgagccg gatcgtcgag gcggcatgat tccgtcgtcg ttggcgccgt acgagacgag
      901 cgcgacagcg cagccgcgtg cgttccgcgc gcctcgagtc gggtaccgcg tgcgacaggc
      961 tcgcgagatg cgacgaggct gcagtgccga gatcgtcgga catcatgtgg tgacctcgtt
     1021 caacgacgca aggccaggcc cgtcggtctc ggcaagccgt cgcgccaggc gcatgcgcgc
     1081 ccggctgaca cgctgccgca tcgccactcc cgtgacgccg cagattgcgg cggcctcgga
     1141 ttggcggagc ccctcgacga cgaccagcag cagcgcttca cgataggcgg ccggcaacga
     1201 cgcgagcgcc gtcgccacgc ggcgctgggc ctcgctggct tccatcactt cagactggga
     1261 tggtctcctc gagccgccag gccagaggcc aatcagatcc gcggcgtgcg actcctcgag
     1321 cagccgcgat cggcagtagc tcacatgcag gtggcgggcg acggtgaaca gccaggcgcc
     1381 cagccgggtg tcgccctgga gccgctgcgc gtgcgtcacg agccgcagcc acgtctcctc
     1441 gagcaggtcc tcggcgacct cccgccggtt cgacagcctg aacagaaagt tgaacagccg
     1501 gacgttgaag acgccgtgca cctcgtcgaa cgcgtcggga tcgcccgatc ggagtcggtt
     1561 gacgaggacg agctctctct cccggtccat caaggtacta acgcggagct caccggatcg
     1621 tgacagcgtc acgttctacc gtcctccgcg ttgtaagggg gtgatgggga ggtgccaatg
     1681 gatctaggtg aggcgttcgc acagatgggg ataatcgcca gagtcacaat cttcatgggg
     1741 gtcgtgccgc tcggcatggg cattgtctac gcgatctcgc cgagcgagca acgcctggcg
     1801 ctgatgcgtc cgctgtcgct cgcggcgatc tttggggctc tcagcggcac gatggccggt
     1861 gtgatcaaca gcctggtggg tatggcccga acccagacgc ctgtcttctc gcctgtggtg
     1921 gcagccggcc tggccgaatc gctggtgccc gtcttctttg gcttcggctg cctgacggtc
     1981 gcctggctgt gcgtcgcggt cggcctctgg agaaaggcct gatccgacgg ctctgtgagc
     2041 aacgcctcag cttgctaccg gcaggagtca ccttgtcgtc ccccaaggcg aacagccggc
     2101 gggcggaagc ggcagccgcg cacgaattac tgatggccag cgcctcctcc ctgttggtac
     2161 actcactgca atagcactca cgtcacgcca gggtccagga attgccagtg gaggtcacga
     2221 tgggacgcaa agccctgtgg attgttggag cggcggtggg attggcactg tatgccgccc
     2281 cggcacacgc gcaggtcagc gtttcgatag gagtcggagg gccctggttc ggcgcgggtg
     2341 tcgtggtcgg cgggccggtc tacggtgctc cctactacta cccgtattac tcctatccct
     2401 atccgtacta ctacccgtat cccgtctact cctatcccta tcccgtttac cgctatcgca
     2461 ccgcgtaccc ttaccgttac cccgcgcggt acggacgcgc gtactacggc gggtccgccg
     2521 tggttcggca ggctcagccg tactaccgcg gtgcgcaagg ccgctactac aacaacggcc
     2581 ccggcgcccc ggcgcagtat cgtcgcggcg cggctcctgc tcagcgcgtt ggccccgctc
     2641 agcgcactgc ccccgcgcag cgcggcaacg ctccgcgcgg aaacggtcag gccgtgcgcg
     2701 tcgcgcgaca gcggtagcgg ccacctcgac caacggccgg tctggatcgc ggctccaatc
     2761 ccagcgcact gcggtcgacg ttctggtgat cgattcgatc cggccgttgt ccgataacta
     2821 actgctgcgg tgtctacggc ccgcccgacg gcgatgcgtt cgcgttccag atgtcgcggg
     2881 ccagcagcca cgttccgtcc tcctgccgtg tgtagatctg caggaacttc ccttcctgcc
     2941 ggattggctg agggacacct ggtgcctcca tggccagtgc gtaggtgccg cggacgtaag
     3001 cgaaatcgcc gctgccgtcg acttccagcg ccacgccatc cgcttcggaa atgaccactg
     3061 gaagcgccct tagccaagct tcgatggcgc ttcgtccctc aacggtggcg ccgtttggcg
     3121 gcatcactac cgcgttgtcc gcgtacagcg ccgcccagcg cgacgggtct cgaccgatcg
     3181 cgctggcgaa ataggagtcg agcgcggctc gcacggcgac tgcatccgcg gctgccagcc
     3241 taccgtcgac accggtgtcc ccatcaggta ctgccggcgc gcgacacgcc tggacgaaga
     3301 gcaccatcag catgatgccg agcagcgatc gaagcctggc aacgaagtcg ggcggcacct
     3361 ggaagggatg cgcgcgatgc agctcggcat a
//
LOCUS       DBNGSH010000761         2325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3480088, whole genome shotgun sequence.
ACCESSION   DBNGSH010000761 DBNGSH010000000
VERSION     DBNGSH010000761.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3480088"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC16CY_17550"
     CDS             <1..228
                     /locus_tag="AC16CY_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3355660.1"
                     /translation="SNGPHPIQRAFIEQQASQCGFCLSGVILTAKAFLDANPRATDAQ
                     IEQALSGVLCRCFTNVRMLEAIRRYAREARA"
     gene            225..>2325
                     /locus_tag="AC16CY_17555"
     CDS             225..>2325
                     /locus_tag="AC16CY_17555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3355661.1"
                     /translation="MTAPLSRDARRALHRAGFSRRDFLKGSGALVVAFGSAAVADRYG
                     VLAQGINGAGSTQLDSWIAIGSDGTVTAYTGKCDFGQGLYTAQMQLVAEELAVPLSRV
                     RLIQCDTSMTPDQGTTSGQQSHPANFNETNLALAAATAREALVGLASTRLGVPAADLI
                     ASDGRVSLKTTSSRSVGYGELVGGRRFNLPLNAAAKRKHPREWSVLGTPVPRVDMREM
                     VEGRFEFVHNVKVPGMLHGRVVRPPEVGAALVRVDDSSVRDLPGIVKVVVKGNFVGVV
                     AQKQWQAVQAAERLEVEWTRGTTTLPPQRDLYRTLRTQPARDTLLVDSGDVDAKLKAA
                     STVLASTYQHPYQMHGSIGSSCAVADVQGEQATVWSATQSVYPTRNTTAMLLGLRPEA
                     VRVIYTRGAGCYGINGADTVSYDAALLSQAAGRPVRVQLSRKDEMAWENYGNAFVIDQ
                     RIGLDRDGNIAVWDYEAWSAARGGRPGYNTPGNVVTGFLAGLNPAPFAPRTPAPPPNT
                     PLNNGSNTAPSYIAGRVRESSNGAGIVASERVLSHRVLSPFFTGPLRAPERLQNTFAH
                     ECFLDEAAAHVKADPVEYRLRHLSHARLSAAVRAAARAARWEPRPSPRPGRRPTGTVS
                     GRGIACVCYEGDNGYVAMVADVDVNQDTGVIHVTRLVVAQDSGPISNPDGMRNQIEGG
                     ALQGLSRALGEEVTWDDR"
BASE COUNT          374 a          815 c          801 g          335 t
ORIGIN      
        1 tccaacgggc cgcatccgat tcagcgcgcg ttcatcgagc agcaggcgtc gcagtgcggc
       61 ttctgcctga gcggcgtcat cctgaccgcg aaggccttcc tcgacgcgaa tccccgcgcg
      121 acggacgcgc agatcgagca ggcgctctcg ggggtgctct gccgctgctt caccaacgtc
      181 cggatgctcg aggccatcag gcgctacgcc agggaggcca gggcatgacg gctccgctct
      241 cccgggacgc tcgacgcgcg ctccatcgcg cgggcttctc gcgccgcgat ttcctcaaag
      301 gatccggcgc cctcgtcgtc gcgttcgggt cggcggcagt ggcggaccgg tacggcgtcc
      361 tcgcgcaggg tatcaacggc gcgggcagca cgcagctcga ttcgtggatt gccatcggca
      421 gcgacggaac cgtcaccgcc tacacgggca agtgcgattt cggacaaggg ctctacaccg
      481 cccagatgca gctcgtcgcc gaagagctcg cggtcccgct cagccgcgtc cgtctgattc
      541 agtgcgacac ctcgatgacg cccgatcaag gcacgacctc cgggcagcag tcgcatccag
      601 ccaacttcaa cgagacgaac ctggcgctgg cggccgccac ggcgcgcgag gcgctcgtcg
      661 gcctggcctc gacccgcctg ggggtgcccg ctgcggacct gatcgcgagc gacggccgcg
      721 tgtcgctgaa gacgacgtcc tcgcgaagcg tcggctacgg cgagctcgtt ggcgggcggc
      781 ggttcaacct tccgctgaat gccgcggcca aacgcaagca cccgcgcgag tggtcggtgc
      841 tgggcacgcc ggtgccgcgc gtagacatgc gcgagatggt cgaaggacgc ttcgagttcg
      901 tccacaacgt caaagtgccc ggcatgctgc acggccgcgt cgtccggccg cctgaagtcg
      961 gcgccgccct cgttcgcgtg gacgacagct ccgtgcgcga cctccccggg atcgtgaagg
     1021 tggtcgtcaa ggggaacttc gtcggggtcg tcgcgcagaa gcagtggcag gccgtccagg
     1081 cggccgaacg gctcgaggtc gagtggacgc gagggacgac gacgctccct ccgcagcggg
     1141 acctgtacag gacgctccgt acccagcccg cgcgcgatac gctgctcgtg gactccggcg
     1201 acgtggacgc gaagctgaaa gccgcatcaa cggtcctggc gtccacctac cagcatccct
     1261 atcagatgca cggatcgatc ggcagctcct gcgccgtcgc cgacgtccag ggcgagcagg
     1321 ccacggtgtg gtccgcgacg cagtcggtgt accccacgcg caatacgacg gcgatgctgc
     1381 tcggtctgcg gccggaagcc gtccgcgtga tttatacgcg aggggcgggc tgctacggca
     1441 tcaacggcgc cgacacggtg agctacgacg ccgcgctgct gtcacaggcc gccgggcgcc
     1501 ccgtgcgcgt ccagttgtcg cggaaggacg agatggcatg ggagaactac ggaaacgcct
     1561 tcgtcatcga ccagcgcatc gggctcgatc gggatggcaa catcgccgtg tgggattacg
     1621 aggcctggtc ggccgcacgg ggcggacggc cgggatacaa cacgccgggg aacgtggtca
     1681 ccggtttcct ggcaggtctc aaccccgcgc cgtttgctcc caggacgccc gcgccgccgc
     1741 cgaatacgcc cctgaacaac ggatcgaata cggcgccgtc gtacatcgcc ggccgggtgc
     1801 gggaatcgtc gaacggggcc gggatcgtcg caagcgagcg cgtgctctcg caccgcgtgc
     1861 tgtccccctt cttcaccggc ccgctgcgtg cgccggaacg gctgcagaac accttcgcgc
     1921 acgagtgctt cctcgatgaa gccgcggcgc acgtcaaagc cgatccggtc gaataccggc
     1981 tgcgccactt gagccacgcg cgcctgagcg cagccgtgag ggccgcggcg cgcgccgccc
     2041 ggtgggagcc gcgtccctcc ccccgccccg gtcgccgtcc gaccggaacg gtcagcggac
     2101 ggggcatcgc gtgcgtctgc tacgaaggag acaacggcta cgtcgcgatg gtggcggacg
     2161 tggacgtgaa ccaggatacc ggcgtcattc acgtgacccg cctcgtcgtc gcgcaggact
     2221 ccggcccgat ctcgaacccc gacggcatgc gcaatcagat cgagggaggc gcgcttcagg
     2281 gcctgagccg cgcgctcggc gaggaagtga cctgggacga ccgca
//
LOCUS       DBNGSH010000762         4757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4159789, whole genome shotgun sequence.
ACCESSION   DBNGSH010000762 DBNGSH010000000
VERSION     DBNGSH010000762.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4159789"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="AC16CY_17560"
     CDS             <1..1240
                     /locus_tag="AC16CY_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR3355662.1"
                     /translation="SAGLLPLGGQVASAWESVSSLREARDRARMDEREKAARLDLLLF
                     QLNEIEKAAPKPGEDEELATTKQVLANAERIQRLCEEAYDALYDGDRAALSSLSQVWK
                     RIGELSTLDDRFAGHLDARDAIKSQLEDLSSFLRRYADGIDASPGRLQQVEDRLALLE
                     RLKRKYGPTLERVIEAGAALGRERDALVRADEIAGGVEQSLAAATGAYLDLARDLSRK
                     RREASVRFASNVESQLGELAMSRTRFEVRFNAADAPEAAWGATGIDEAEFFVSPNPGE
                     DLRPLAKIVSGGELSRVMLALKTMAAAGQAGKTLIFDEVDAGIGGRVAEVVGRKLQQL
                     GKRFQVICITHLPHIASRASAHFHVEKAVRGARTVTAVQRLDEPGRIEEIGRMIGGRT
                     LSAPVLASAREMLGLQAKGK"
     gene            1281..2672
                     /gene="miaB"
                     /locus_tag="AC16CY_17565"
     CDS             1281..2672
                     /gene="miaB"
                     /locus_tag="AC16CY_17565"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR3355663.1"
                     /translation="MAKKYLIETFGCQMNVHDSERMAGLLDSAGYEPTTEDVDADVIV
                     INTCSVREHAEEKLYTRLGQLRVLQEETGRNPVVAVTGCVAQQEGGALLRNTNGKVVD
                     LVVGTQRLKMLPLLVEAASDSPFPSIDVAAWDDVTFPLGITRRSDPVKAYVTIIEGCN
                     DHCAFCVVPYTRGHERMRPKADILADVRDAVSSGRKEIQLLGQIVNHYAAPDDASCDF
                     AQLLAEVNDVPGVERIRFASPHPRHTGTRLIQAVRDLPRVCKHFHLPVQSGSTRVLAA
                     MRRRHTREEYLDLVGRIREEIPGVALSTDMIVGFPGETGADFDETLSLTSAARYHSMF
                     SFKYSPRPNTLASKRLSDDVSAEEKTARIVALQQLQRDIQTELHGQAVGTEVEVLVDS
                     VSRRRSEELSGRTSGNTVVNLPMPSDTPLDGGPTWVGRSVGVRITRAGPHSLWGVPLP
                     AGARKGTETSHAD"
     gene            2662..3147
                     /locus_tag="AC16CY_17570"
     CDS             2662..3147
                     /locus_tag="AC16CY_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR3355664.1"
                     /translation="MLIEMSIKGLMVDPVAGTPIVILKDKLGERVLPIWVGVPEANAI
                     ALQIENVATPRPMTHDLLRNIIADLEGRVDRVVVSDLKDNTYFAIIHLTVRGERVAVD
                     ARPSDAIALALRTRSPILVEEAVIDSAKSTDFTSEPADSERLQKWLESLDPEELGKYK
                     M"
     gene            3327..4100
                     /locus_tag="AC16CY_17575"
     CDS             3327..4100
                     /locus_tag="AC16CY_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3355665.1"
                     /translation="MIVAIANQKGGVGKTTTAVNLAAALAMRGRKTLLIDLDPQANSS
                     ISFLEHGSISKSMYDAIADPSCDLASVVQPAATTKNLFVAPARIGLAKLESRLVGELD
                     AHFKLKDKLVKVARDYAHIVIDCPPALGLLTVNALVAATHLLIPIQSSYFALEGTDDL
                     LETIEKVRQRPNPGLRILGVVITMHDKRTALARDIREQIQKVFGGKVFKTVISKSVRL
                     EESPAYKESIFTFAPESSGATDYYSLCEEVIERVEARTA"
     gene            4078..>4757
                     /locus_tag="AC16CY_17580"
     CDS             4078..>4757
                     /locus_tag="AC16CY_17580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3355666.1"
                     /translation="MSKRGLPETLRMRHDEHYVESLAASAGEPVGRLVPIDLLDPNPG
                     QPRQVMGDLSELMASVAEKGIIEPLVVRQRGSRYQIIAGERRYHAAVQVGLREVPVVL
                     REVDDVEVLELALVENLQRKDLTAFEEAEALQQLAENCGHTHEDLARKLGKSRTSITE
                     SLSLTTMPEDVRNLCRLADITAKSTLLQVVRQSDPKKMVALVEKLAGEGTVTRQSARR
                     ETAKAKLGR"
BASE COUNT          874 a         1490 c         1633 g          760 t
ORIGIN      
        1 gtcggccggg ctcctgccgc tcggcggcca ggtcgcctcg gcctgggagt cggtctcgtc
       61 gctccgggag gcgcgcgatc gtgcgcgcat ggacgagcgc gagaaagcgg cgcgtctcga
      121 tctgctcctg ttccagttga acgagatcga gaaggccgca cccaaaccgg gcgaggacga
      181 ggagctcgcc acaacgaaac aggttctcgc caacgccgag cgcattcaac gcctgtgcga
      241 ggaagcgtac gacgcgctgt atgacggcga ccgcgcggcg ctgtcgtcgc tgtcccaggt
      301 atggaaacgg ataggcgagc tttccacgct cgacgatcgc ttcgcggggc atctcgacgc
      361 tcgcgacgcc atcaagtcgc agctcgagga cctctcgtcg tttctgcgcc gctacgccga
      421 tggcatcgac gcgtcaccgg gccggctgca gcaggtagag gatcgcctcg ctcttctcga
      481 gcgtctcaag cggaagtacg gccccacgct cgaacgcgtg atcgaggccg gcgccgcgct
      541 gggccgcgaa cgggacgcgc tcgtccgggc cgacgaaatc gccggcgggg tggagcagtc
      601 gctggccgcc gcgaccgggg cctacctgga cctcgcgagg gatctgtcac gaaagcggcg
      661 cgaggcgtcg gtccggttcg cttcgaacgt agaatcacag ctgggggagc tggcgatgtc
      721 gcggacgcgt ttcgaggtcc gcttcaatgc ggcggacgct cctgaagccg cgtggggtgc
      781 gaccgggatc gacgaagccg agttcttcgt ctcgccgaac ccgggcgagg atctgcggcc
      841 tctcgcgaag atcgtctccg gcggcgagct gtcccgcgtg atgctggcgc tcaagacgat
      901 ggctgccgcc ggccaggcgg ggaagacgct gatcttcgac gaagtggacg cgggcatcgg
      961 cgggcgggtc gcggaggtcg tgggcaggaa gctgcagcag ctcggcaagc gcttccaggt
     1021 gatctgcatt acgcacctgc cgcacatcgc ttcgcgcgcc tctgcgcact tccacgtcga
     1081 gaaggccgtt cgaggagccc gcaccgtgac cgccgttcaa cggctcgacg agccgggtcg
     1141 tattgaagaa atcggacgca tgatcggcgg acgaacgctg agcgcgccgg tgctggcgag
     1201 cgcgcgcgag atgctcgggc tgcaggcgaa aggaaaatag aaggcgaaag gcgaaaggcg
     1261 aaagcgaaac ccgcgaagac atggccaaga aatacctgat tgagaccttc ggctgccaga
     1321 tgaacgttca cgactcggag cgcatggctg gcctgctcga ttcggcaggc tacgagccga
     1381 cgaccgagga cgtcgatgcc gacgtgatcg tcatcaacac gtgcagcgtg cgcgagcatg
     1441 ctgaagagaa gctgtacacg cggctggggc agctgcgcgt tctgcaggaa gagaccggcc
     1501 gcaatccggt cgtggccgtg accggctgcg tcgcgcagca ggaaggcggg gcgctgctcc
     1561 gcaacaccaa cgggaaggtg gtggacctcg tagtgggcac gcagcgtctc aagatgctgc
     1621 cgctgctcgt ggaggcggcg tcggattcgc cgttcccgtc gatagacgtc gccgcgtggg
     1681 acgacgtgac gtttccgttg gggatcacgc gccgctccga tccggtgaag gcctacgtga
     1741 cgatcatcga aggctgcaac gaccactgcg cgttctgcgt cgtcccatac accagggggc
     1801 acgagcggat gcggccgaag gcggacatcc ttgcggatgt tcgcgacgcc gtgtcgtccg
     1861 gacggaagga gattcagctg ctcgggcaga tcgtcaacca ctacgccgcg cccgacgatg
     1921 cctcgtgcga ctttgcccag ttgctggccg aggtcaacga cgtgcccggt gtcgagcgta
     1981 ttcgtttcgc gagtccccac ccgcgccata cggggacgcg gctgatccag gcggtgcggg
     2041 acctgcctcg cgtctgcaag cactttcacc tcccggtgca gtccgggtcg acgagggtcc
     2101 tggccgcgat gcggcggcgt cacacacggg aagagtatct ggacctggtg ggccggattc
     2161 gggaagagat cccgggagtc gcgctgtcaa cggacatgat cgtgggtttc ccgggcgaga
     2221 cgggcgccga tttcgacgag acgctctcgc tcacctcggc ggcgagatat cacagcatgt
     2281 tctcgttcaa gtactcgccg aggccgaaca cgctggcctc gaaacggctc tccgacgatg
     2341 tgagcgccga ggagaagacc gcccggatcg tcgccctcca gcagctccag cgggacatcc
     2401 agacggagct tcacgggcaa gcggtgggga cggaggtcga ggtgctggtc gattcggtga
     2461 gccggcggcg ctccgaagag ctgtccggac ggacgtcggg gaacaccgta gtgaatctgc
     2521 ccatgccgtc cgatactcct ctcgacggcg ggccgacgtg ggtcgggagg tccgtcggcg
     2581 tccggattac gcgcgccgga ccgcacagcc tgtggggtgt gccgctcccg gccggggcac
     2641 gcaagggcac ggagacgagt catgctgatt gaaatgagca tcaaagggtt gatggtcgat
     2701 cccgtcgccg gcacgcccat cgtgattctc aaggacaagc taggcgagcg cgtgctgccg
     2761 atctgggttg gtgtgccgga ggcgaacgcg atcgcgctgc agatcgagaa cgtcgcgacg
     2821 ccgcgcccga tgacgcacga cctgctccgg aacatcatcg cggacctcga ggggagggtc
     2881 gatcgcgttg tggtgagcga cctgaaggac aacacgtact tcgccatcat ccacctgacc
     2941 gtccgggggg aacgcgtggc ggtagatgcg cgcccgagtg atgccattgc gctcgccctc
     3001 cggacgcgtt ctcccatcct cgtcgaggaa gccgtcatcg acagcgccaa gagcaccgat
     3061 ttcacgtccg agcccgccga cagcgagcgt ctccagaagt ggctcgagag cctggatccc
     3121 gaggagctcg gcaagtacaa gatgtgaatg tcggccggcc gacattgatc gccgattgac
     3181 acgccccgga cgcgtcccta caatgggcgc accccttccc agatttgcgc acacgtggga
     3241 tacctttcga catacgtctc aggccggccc ttatctgagc cggtgaccgc tggtctggct
     3301 ctcctccacg cctctccatc gacaccatga tcgttgccat tgccaaccag aagggtggcg
     3361 tgggaaagac cacgaccgcg gtcaacctgg ccgcggccct ggccatgcgc ggacggaaga
     3421 ccctactgat cgatcttgac ccgcaagcca acagcagcat ctcgtttctc gagcacggct
     3481 caatctccaa gagcatgtat gacgcgatcg ccgacccgtc gtgcgatctc gcgagcgtcg
     3541 tgcaacccgc ggcgacgacg aagaacctgt tcgtcgctcc agcgcgcatc ggcctggcga
     3601 agctggagtc gaggctggtg ggggagttgg atgcgcactt caagctgaag gacaagttgg
     3661 tcaaggtcgc gcgggactac gcccacatcg tgatcgattg ccctccggcg ctcggccttc
     3721 tgaccgtgaa cgcgctcgtt gccgccacgc acttgctgat tcccatccag tcgtcctact
     3781 tcgcgctcga gggcaccgac gacttgctcg agacgatcga aaaagtgcgc cagcggccaa
     3841 accctggact ccggatcctg ggcgtcgtga tcacgatgca cgacaagagg acggcgctgg
     3901 ctcgcgacat ccgcgagcag atccagaagg tgttcggcgg gaaggtgttc aagacggtca
     3961 tcagcaagag cgtccggctc gaggagagcc ctgcgtacaa ggaatcgatc ttcacgttcg
     4021 cgcccgagtc gagcggtgcg accgattact acagtctctg cgaggaggtc atcgagcgtg
     4081 tcgaagcgcg gactgcctga aacgctacgg atgcgtcacg acgaacacta cgtcgagtcg
     4141 ctcgcggcct cggcggggga gcccgtcgga cggctcgtgc cgatcgatct gctggatccg
     4201 aaccctgggc agccccgcca ggtgatgggc gacctgtccg agttgatggc ctcggtggca
     4261 gagaagggca tcatcgagcc gctcgtcgtg cggcagcgag ggagccgcta ccagatcatc
     4321 gccggcgagc ggcgctacca tgccgccgtt caagtgggcc ttcgggaagt gccggtcgtg
     4381 ctccgtgaag tcgatgacgt ggaggtgctc gagctcgcgc tggtcgagaa cctccagcgg
     4441 aaggacctga cagcgttcga ggaggcggag gcgctgcagc agctcgccga gaactgcggg
     4501 cacacgcacg aggacctcgc gcgcaagctc ggaaagtcgc gcacgtcgat caccgaatcg
     4561 ctgtcgctga cgacaatgcc ggaggacgtc aggaacctat gtcggctggc cgacattacg
     4621 gccaagtcga cgctgctgca ggtggtccgc cagtccgatc ccaagaagat ggtggcgctc
     4681 gtggagaagc tggccggtga aggcacggtc acgcgtcaga gcgcacgcag ggaaaccgcg
     4741 aaggccaagc tgggacg
//
LOCUS       DBNGSH010000763         1678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_593968, whole genome shotgun sequence.
ACCESSION   DBNGSH010000763 DBNGSH010000000
VERSION     DBNGSH010000763.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_593968"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1076)
                     /locus_tag="AC16CY_17585"
     CDS             complement(189..1076)
                     /locus_tag="AC16CY_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355667.1"
                     /translation="MRHLRLLVALSLAAAALSTIPFAAGQQPTGDPFSTEIRKVKDGL
                     YVIPGYDGAVTGGNVAVRVTSEGTIIVDDRLPPSSAEIAGKVRSVTSQPIKYVLSTHG
                     HGDHTGGHPEFITVAEIVAHRNNRANMIRSKLAAPPRLVFTDQAAVFLGNVEAQAIHL
                     GRGHTNGDAVIYFPDLRTIHTGDLVVWGKRTDGSVLTPFVDRASGGSLVAWITTLDGV
                     LKLDFDTAIPGHGPLLTKDHIRTFHRNLVTLRQRLTEVVKAGAKKEELGARLKTDDLG
                     WPFPPERLNELWDEFGAGR"
     gene            1173..>1678
                     /locus_tag="AC16CY_17590"
     CDS             1173..>1678
                     /locus_tag="AC16CY_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355668.1"
                     /translation="MPKPEAPDELQRLTRELENFQDIARQLVPLPGEVPTLQGFDIWG
                     GSLALAGSVGGDHITYVDFKTRFDLQARIERARADGRLDVVENLERCQHKGGVALIDV
                     AGHRMTDALLAAMLHQAFLLGAIYELDRYGQITRRLFEHLNTRFYQSSGMSKFVSLIY
                     GEISDDAK"
BASE COUNT          283 a          559 c          548 g          288 t
ORIGIN      
        1 gagcgcgagg atttcgtagg ggcccagacg cgttccggca ctcaaagcca tcggggtctc
       61 ccggtgtcag cgggccctac tatagctgag aagagtggtc cgctcaggtc cggctgaagc
      121 cggacacaac gaatgtccag cgcatgtcca gtgcatccgt agtgtccggc ttcagccgga
      181 ccgtggggtc agcgtcccgc gccaaactcg tcccacaact cgttgaggcg ttccggcggg
      241 aacggccacc cgaggtcgtc agtcttcagc cgcgccccca gctcctcctt cttggcgccc
      301 gccttgacga cctcggtcag ccgctgccgc agcgtgacca ggttccggtg gaacgtgcgg
      361 atgtggtcct tggtcagcag gggtccgtgg cccgggatgg ccgtgtcgaa gtcgagcttc
      421 agcacgccgt cgagcgtcgt gatccacgcg acgaggctgc cgccgctggc gcgatcgacg
      481 aacggcgtga ggacgctgcc gtcagtgcgc ttgccccaga cgaccaggtc gcccgtgtgg
      541 atcgttcgca ggtcgggaaa gtagatgacg gcgtcgccgt tggtgtgtcc tcgccccagg
      601 tggatcgcct gcgcctccac gttgcccagg aacacggccg cctgatcggt gaagacgagc
      661 cgcggcggcg ccgcgagctt gctgcggatc atgttcgccc ggttgttccg gtgggccacg
      721 atctcggcca ccgtgatgaa ctccgggtgt ccgccggtgt ggtcgccgtg cccgtgcgtg
      781 ctcagcacgt acttgatcgg ctgcgaggtg acggaccgca ctttgccggc gatttccgcg
      841 gaggacggcg gcaggcggtc gtccacgatg atcgtgccct cgctcgtcac gcgaacggcc
      901 acgttgccgc ccgtcacggc gccgtcgtag ccggggatga cgtacagccc gtccttcacc
      961 ttccggatct cggtcgagaa cgggtcgccc gtcggctgct gccccgccgc gaatggaatc
     1021 gttgacaggg cggccgcagc caggctcaga gcgacgagca accggagatg tctcatggga
     1081 cgaagcgtaa ttcaaaactc aaaactcaag actcgaaaag tcaaatacca aatggctctc
     1141 cacgaccggg tatgatctca gtgcaaccgt gaatgcctaa accggaggcc cccgacgagc
     1201 tgcagcgact gacgcgagag ctcgaaaatt tccaggacat cgcgcgtcag ctcgtgcctc
     1261 tgccgggcga agtgccgacc ctccagggct tcgacatctg gggcgggtcg ctcgcgctgg
     1321 ccggatcggt cggcggcgac cacatcacgt acgtggactt caagacgcgg ttcgacctgc
     1381 aggcccgcat cgagcgcgcc cgcgccgacg gccggctgga cgtcgtcgaa aacctggaac
     1441 ggtgccagca caagggcggc gtcgcgctga tcgacgtcgc cggtcatcgc atgaccgatg
     1501 ccctgctggc cgccatgctc caccaggcgt tcctgctcgg cgccatctac gagctggatc
     1561 gctacggcca gatcacgcgc cgcctgttcg agcacctcaa cacccggttc taccagtcgt
     1621 cggggatgag caagttcgtc tcgctcatct acggagagat ctccgacgac gcgaagtt
//
LOCUS       DBNGSH010000764         2306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4498914, whole genome shotgun sequence.
ACCESSION   DBNGSH010000764 DBNGSH010000000
VERSION     DBNGSH010000764.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4498914"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="AC16CY_17595"
     CDS             complement(<1..793)
                     /locus_tag="AC16CY_17595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355669.1"
                     /translation="MNGPALDVRGLSKSYGPRGLTFTVPRGVISGLLGPNGSGKTTTL
                     RILMGLAHAQGGSAEVLGVPAGSSHEIFRRVAFVPEVKDLYPFARAGEMIRLTRGFFP
                     NWDHELEKRLVDAFAIPLRMRCPKLSKGTKAKLALLLAVCRRVELLVLDEPTEGLDPL
                     AIEETLRLLVEQAGHGVTVFFATHQLPEVEQIADHVVMIKDGTCVAAGDLDDLKSRYR
                     RVRCVIETSDVEVPAAMAGWRRDGRFLSGFSADDGDTLGRQLAGSG"
     gene            complement(790..1176)
                     /locus_tag="AC16CY_17600"
     CDS             complement(790..1176)
                     /locus_tag="AC16CY_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3355670.1"
                     /translation="MLFRVNPQSGVPIYLQLIDQVRHAVEIGALRPGDQLPGIRKVAE
                     ELVMNPNTVAKAYREMEHAGLIALRHGAGAFVADTPARGQQAVRKGRAVVHRAVAALR
                     DLGLDEGAIRRLVESELAETEQEARR"
     gene            1462..2283
                     /locus_tag="AC16CY_17605"
     CDS             1462..2283
                     /locus_tag="AC16CY_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355671.1"
                     /translation="MAASADDALASLADATPDVILTSSLLSEREDEQLTAHLRAVPDL
                     DHLPILTIPPLVEMTSEKKPERRRWYQLFRARQQRAPQPAYDFSAISTRIEEALEQSK
                     YDAPESEIERPARLVLLATSRTLLLEAGKPQQVASTPTMSLVRYGDDLPADERKWRSR
                     ARRWNGDELPWLSGVQLAWGASTFPTLRLLNISSTGLLLESSARLTQGNKAGFRLAGS
                     DHEQLIVHASVVRADPLVEDRSETKYVVAATFDWPFSSLGPVRSLRDLRRLDRIV"
BASE COUNT          381 a          774 c          752 g          399 t
ORIGIN      
        1 ttcccgatcc ggccagctga cgcccgagcg tgtcgccgtc gtcggccgag aacccggaca
       61 ggaagcgccc gtcgcgacgc cagcccgcca tggcggccgg cacctccacg tcgctcgtct
      121 cgatgacgca acgtacgcgg cgatagcggc tcttgagatc gtcgaggtcg cctgccgcaa
      181 cgcacgtgcc gtccttgatc atcacgacgt ggtcggcgat ctgctccact tccggcagct
      241 ggtgtgtcgc gaagaagacg gtcacgccgt gtcccgcctg ctcgaccagc aggcggagcg
      301 tctcctcgat cgcaagcggg tcgaggccct cggtcggttc gtcgagcacg agcagctcga
      361 ctcggcggca gaccgcgagc aggagcgcca gcttcgcctt ggtgcccttc gacagcttcg
      421 ggcacctcat gcgaaggggg atcgcgaacg cgtcgacgag ccgcttctcg agctcgtgat
      481 cccagttcgg gaagaagccg cgcgtaaggc gaatcatctc gccggcgcgg gcgaacgggt
      541 agaggtcctt gacctccgga acgaacgcga cccggcggaa gatctcgtgc gagctgcccg
      601 cgggaacacc gagcacctcc gccgacccgc cctgcgcgtg cgccagcccc atcaggatgc
      661 gcagcgtcgt cgtcttgccc gaaccgttgg ggccgagcag tccgctgatg acgccgcgcg
      721 ggacggtaaa ggtgagcccg cgagggccgt acgacttgga aagacctcga acgtcgaggg
      781 ccggaccgtt catcgacgcg cctcctgctc tgtttccgcg agctccgatt ccacgagacg
      841 ccggatcgcg ccttcgtcca ggccgaggtc gcgcagcgcc gcgaccgcgc gatgcacgac
      901 ggccctgccc tttcgcacgg cctgctgacc gcgcgccggc gtgtcggcga cgaacgcgcc
      961 ggcgccgtgg cgcagggcga tgaggcccgc gtgctccatt tcgcggtacg ccttggcgac
     1021 cgtgttgggg ttcatgacca gctcttcggc caccttccgg atgcccggga gctggtcgcc
     1081 gggccggagg gcgccgatct cgacggcgtg acgcacctgg tcgatgagct gcaggtagat
     1141 ggggacgccg gactgggggt tgacgcgaaa tagcactact gtactactac agtagcacag
     1201 tcacgccgca tgtcaagtcc gtcgaatcaa gtgtctcgtt gtagcccttc atggaacacg
     1261 ctgcgtttca taacggtacg gagcgcaccc ggatctccta cgcacaaggg gttttgagtc
     1321 gggtcccaga cttgctcatc cgtttgcgct ttcggtgtca ctggggggtc aatgcgacga
     1381 atcttggcga tcgagcccga cggggggcgc agggtaacac ttcgacgtct cgtccgtgaa
     1441 catgtgcgcg ctgaggtcgt cttggccgcc tcagccgatg acgcgctcgc cagcctggct
     1501 gatgccacgc cggatgtgat cctgacgtcc agtcttcttt cggaaaggga ggacgaacag
     1561 ctgaccgccc acttgagggc ggtgcctgat ctcgaccacc tgcccatcct cacaattcct
     1621 ccgctcgtcg agatgacgag cgagaagaag ccggaacgac gacgctggta ccagctgttt
     1681 cgcgcgcgcc agcagcgcgc gccgcagccc gcctacgatt tcagcgccat ctcgacgcga
     1741 atcgaagagg cgctcgagca atcgaaatac gacgcccctg aatcggagat cgagcgcccg
     1801 gccagactcg tgcttctcgc gacaagcagg acgcttctgc tcgaagcggg caagccccag
     1861 caggttgcgt ccacgccgac catgagcctc gtgcgctatg gtgatgacct cccggccgac
     1921 gagcgaaagt ggcggtcccg cgcgcgccgc tggaacggtg acgaactgcc ctggctttcg
     1981 ggcgttcagc tcgcatgggg agcgagcacg ttcccgacgc tgcgtcttct caacatctct
     2041 tcgaccggac ttctccttga atcgagtgcc cgtctgacgc aggggaacaa agccggcttt
     2101 cggcttgccg gctcggacca cgagcagttg atcgttcacg ccagcgtggt tcgcgccgac
     2161 ccgctggtgg aggaccgatc tgagacgaag tacgtcgtcg cggccacgtt cgattggcca
     2221 ttctcgtcgc tgggccccgt gcgctcgttg cgcgacttac gccggctcga tcgcatcgtg
     2281 tgaccggcgg cgccgattgg cgttat
//
LOCUS       DBNGSH010000765         4133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1103783, whole genome shotgun sequence.
ACCESSION   DBNGSH010000765 DBNGSH010000000
VERSION     DBNGSH010000765.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1103783"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..548
                     /locus_tag="AC16CY_17610"
     CDS             30..548
                     /locus_tag="AC16CY_17610"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3355672.1"
                     /translation="MRRPKRRAVTRGSRRNSRAKARKRPKPPSAPPTETARQTKASGL
                     APGMHAINTYLAVANVNASMSFLEHALGFTRGVVLPDPDGQLRYAEMRQGDSVVMLVR
                     RGDRATASGGAAALYTYVEDVDRATTDARKAGAGVTDAEDQPWGDRTAVVTDPDGYRW
                     VLATFKKLVPFT"
     gene            complement(695..1741)
                     /locus_tag="AC16CY_17615"
     CDS             complement(695..1741)
                     /locus_tag="AC16CY_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR3355673.1"
                     /translation="MTRRSIVLALAFVLSAPVASSAQGAGSSTRRVAVDTVVGVQDYF
                     DDAGAWKTQVILDPFTTVEAAPGLQVSFRPLLWHVMTGDWEAMVVHASLRYEFQKGGK
                     WRVEGGKFTSPIGLGMTENRASINDSVIWWHRGYYSYLPSIGGGAAPHALISSIYPIG
                     IQVNRSSDRWDARAAFIDRAPTDFFHVEGAPARPNGVVGAGVSPRQGLRIGAAAAWGM
                     SGDATVSEPYTLVNVEGEYAFGYTKVSGEWTRDRFRTPAGDRLAHGLTLQVRQTITPR
                     VYVHSRATAIESPVTTAAGEVQPATSWYVDTTAGYLLTSEATIRVAHAAIHRWNRPFD
                     NQVGVSIVWSRRWW"
     gene            complement(1738..3480)
                     /locus_tag="AC16CY_17620"
     CDS             complement(1738..3480)
                     /locus_tag="AC16CY_17620"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3355674.1"
                     /translation="MSPRAGASSPRLVVKVLGFAFAVIACVIIAVFVLFSWQTNVRLT
                     KAVADNMEASQLRFAGIEERLRREHTLQAIALAENPTLKAAVDTYYSERGSGLVLDQL
                     QSTIETELAKLQRMMDVPALSVTDARGTILASAGPAWGDWRPGTRLPVRVEADGAPVD
                     AVVSRDGRPYLATAVPLVLGRDVIGEFFVASPLDDAYATGLAKEFRADIAVLIDGRVI
                     ASSAPDVLRRQLEAISLPLSGSVVLGSDEFVVRRLSAVDRASVYALSSVTAAARAVTS
                     EAGWVLLLAGAGALLLAVGGSWWLARTVAGPIHTLTSSVARMAKNRDFAQPLPHSGGS
                     RELDTLALTFDELRAAVALAESESEAAYLGVIGALATALDARDPYTAGHSQRVADLSV
                     AIARQMQLSSADIETLKLGALLHDIGKIGVSDAILRKPGTLTPDEFEHIKLHPTLGAR
                     ILRPLKFLDAQLAIVELHHERPDGRGYPHGLKGDEIPLFARIVHVADAFDAMTSARAY
                     RAARPVAAAVAELWRLIGVDFDPQVVQAMAALPVALTEPAQAPVTPGSLVRFPSRPVS
                     FVDQVTQFMLRRAE"
     gene            complement(3477..>4133)
                     /locus_tag="AC16CY_17625"
     CDS             complement(3477..>4133)
                     /locus_tag="AC16CY_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3355675.1"
                     /translation="ALLGGNSQQAIGARAADAGAISGRVHVVSHPTRRLATAGVYPGR
                     IVNVQAERDVSELDNVIVFVDTDVRVDSAARRVTIRQTGEEFVPHVAAVTTGSTVDFP
                     NDDLIFHNVFSLSRAATFDLGRYPRHSTKSRTFTKPGVVKVFCHLHSHMSAIVRVFSH
                     PYFAIPGRDGRFRLPDVPAGPHRVTAWHERAGEVTHSTVVESGATAELSFSLPLTDPE
                     "
BASE COUNT          705 a         1388 c         1428 g          612 t
ORIGIN      
        1 aagttctggc ccaaaggata gatcaaccta tgagacgacc aaaacgccgc gccgttaccc
       61 gcggttcacg ccgaaattcg agagcgaagg ccaggaaacg cccgaagccg ccttccgcgc
      121 cgccgacgga gaccgcccgc cagacgaagg cgagcgggct tgctccgggc atgcacgcga
      181 tcaacaccta cctcgccgtg gcgaacgtca acgcgtcgat gagttttctg gaacacgcgc
      241 tcggcttcac gcgcggcgtc gtcctgccgg accctgacgg tcagctgcgg tacgcggaaa
      301 tgcggcaggg cgattcggtg gtgatgctcg ttcggcgcgg cgatcgcgcc acggccagcg
      361 gcggagccgc cgcgctctac acctacgtgg aagacgtcga tcgcgcgacg accgacgcgc
      421 ggaaagccgg cgccggcgtg accgacgccg aagaccagcc ctggggcgac cggaccgccg
      481 tcgtcacgga tccggatgga tatcggtggg tgctggccac gttcaagaag ctggtgccgt
      541 tcacgtaggt cagactagcc aaggactccg cccttgatat ccccgcgaag cccttggcta
      601 gtcggcagca tctcttaggc gcgctccgcg cgcctagccc gttatgcgct cgtaacaacg
      661 agtacgaagt tcgcgctcga ggccgcataa cgggctacca ccagcgtctc gaccagacga
      721 tcgagacgcc aacctgattg tcgaaggggc ggttccaccg atggatggcg gcatgcgcca
      781 ctctgatggt cgcctcggac gtgagcaggt agccggccgt cgtgtcgacg taccacgacg
      841 tggcaggctg aacctcgccg gcggcggtcg tgacgggtga ctcgatggcc gtggcccggc
      901 tgtgcacgta cacacggggc gtgatcgtct gcctgacctg cagcgtcagg ccgtgggcga
      961 gacgatcgcc ggcgggtgtg cggaagcggt cgcgggtcca ctcgccgctc accttcgtgt
     1021 acccgaaggc gtactcgcct tcaacgttga cgagcgtgta cggttcgctg acggtggcgt
     1081 cgccggacat cccccacgcc gcggcggcgc cgatgcgcag accctgccgg ggcgagactc
     1141 cggcgcccac gacgccgttg ggccgggcgg gcgcgccctc gacgtggaag aaatcggtcg
     1201 gcgcgcgatc gatgaacgcg gcccgcgcgt cccagcgatc ggaagacctg ttgacctgga
     1261 tcccgatcgg gtagatcgac gagatgagcg cgtgcggggc tgcgccgccg ccgatggatg
     1321 gcaggtagct gtagtagccg cggtgccacc agatcacgga gtcgttgatg ctggcccggt
     1381 tttccgtcat gccgagtccg atgggcgacg tgaacttgcc gccctcgact cgccacttgc
     1441 cgcccttctg aaactcgtac ctgagggagg cgtgcacgac catcgcctcc cagtcgcccg
     1501 tcatcacatg ccagagcagc ggcctgaagg acacctgcag tccgggagcg gcctcgaccg
     1561 tcgtgaacgg atcgaggatc acctgcgtct tccacgcgcc ggcatcgtcg aagtagtcct
     1621 ggacgccgac gacggtgtcg acggccacgc ggcgtgtgga tgacccggca ccctgtgcgc
     1681 tcgacgcgac gggcgccgac aggacgaagg cgagcgcgag cacgatcgaa cggcgcgtca
     1741 ctcggctctc cggagcatga actgcgtcac ctgatcgacg aacgacaccg gccgcgacgg
     1801 aaaccgcacg agcgagcccg gcgtcacggg cgcctgcgcg ggctcggtga gggcaaccgg
     1861 cagcgccgcc atcgcctgca cgacctgggg atcgaaatcg acgccaatca ggcgccacag
     1921 ctcggcaacg gcggccgcaa cggggcgggc cgcgcggtac gcgcgcgcgc tggtcatggc
     1981 atcgaaggca tcggccacgt gcacgatccg cgcaaacaac ggaatctcgt ctcccttgag
     2041 tccgtgcggg tacccgcggc catccggccg ctcgtgatgc agctccacga tggccagctg
     2101 cgcgtcgaga aacttgagcg gcctcagaat cctggcgccg agcgtcgggt ggagcttgat
     2161 gtgctcgaac tcgtccggtg tcagggttcc cggcttgcgg aggatggcgt cgctgacgcc
     2221 gatcttgccg atgtcgtgaa gcagcgcgcc aagcttcagc gtctcgatat cggcgctcga
     2281 cagctgcatc tgccgggcga tcgccaccga caggtcggcc acacgctgcg agtgaccggc
     2341 ggtgtacggg tcccgtgcgt cgagcgcggt cgccagcgcg ccgatgacac cgaggtacgc
     2401 ggcttccgat tccgactcgg cgagtgccac cgcggcacgg agctcgtcga aagtcagcgc
     2461 gagcgtatcg agctcgcggc tgccgccgct gtgcggaagc ggctgcgcga agtcgcggtt
     2521 cttggccatc cgcgcaacgg agctcgtcaa cgtgtggatc ggaccggcga cggtacgcgc
     2581 cagccaccag ctccctccga cggcaagcag cagcgcgccg gccccggcca gcaggagcac
     2641 ccagcccgct tccgacgtga cggcgcgagc ggcggccgtc accgagctca acgcgtagac
     2701 gctcgccctg tcgacggcgg acaggcggcg aacgacgaac tcgtcgctgc cgagcacgac
     2761 ggagccgctg agcggcagcg atatggcttc gagctgccgg cgcagaacgt cgggcgcgga
     2821 gctcgcgatg acccgcccgt cgatgaggac ggcgatgtcc gcgcggaatt ccttcgccag
     2881 gccggttgca tacgcgtcgt cgagaggaga agcgacgaag aactcgccga tgacgtcgcg
     2941 gcccagcacg aggggtacag cggtcgccag gtacgggcgc ccgtcgcgcg agaccacggc
     3001 atccaccggc gcaccgtccg cctccacgcg gacgggcaac cgcgtccccg gccgccagtc
     3061 accccaggct ggtccggcgc tcgcgaggat ggttccccgg gcgtcggtca cggacagcgc
     3121 cggcacgtcc atcatgcgct gcagcttcgc gagctcggtc tcgatcgtcg actgaagctg
     3181 atcgagcacc aggccgcttc cccgctccga gtagtaggtg tctactgcgg ccttgagcgt
     3241 tgggttctcg gcgagcgcga tggcttgaag ggtgtgctcg cgcctgagcc gctcttcgat
     3301 gcccgcgaag cgaagctggc tcgcctccat gttgtcggcg acggctttcg tgagacggac
     3361 gttggtctgc cacgagaaca gcacgaacac cgcgatgatg acgcacgcga tgacggcgaa
     3421 tgcgaagccg aggaccttga cgacgagccg gggcgagctg gcaccggcgc gcgggctcat
     3481 tcgggatccg tcaacggcag cgaaaagctc agttcggccg tcgcgccgct ctccacgacg
     3541 gtcgagtgcg tgacctcgcc ggcccgttcg tgccacgcgg tcactcgatg ggggccggcc
     3601 ggaacgtcgg gaagcctgaa ccgcccgtcg cgccctggaa tggcgaagta cggatgagag
     3661 aacacgcgca cgatcgcgct catgtgcgag tgcagatggc agaacacttt cacgacgcct
     3721 ggcttggtga aagtgcgcga cttcgtggag tgccgagggt agcgcccgag atcgaacgtc
     3781 gctgcccggg acagcgagaa gacgttgtgg aagatgaggt cgtcgttggg aaaatcgacg
     3841 gtcgatcccg tcgtcacggc cgccacgtgc gggacgaact cctcgcccgt ctgccggatg
     3901 gtgactctcc tcgccgcgga gtcaacccgc acgtcggtgt cgacgaagac gatgacgttg
     3961 tcgagctcgg agacgtcgcg ttcggcctga acgttgacga tccgaccggg atagacgcca
     4021 gccgtcgcca gccgcctcgt gggatgtgac accacgtgca cacggccgct gatggcgcca
     4081 gcgtcagccg cccgtgcgcc gattgcctgc tgtgagttgc cgcccagcag cgc
//
LOCUS       DBNGSH010000766         2361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2292741, whole genome shotgun sequence.
ACCESSION   DBNGSH010000766 DBNGSH010000000
VERSION     DBNGSH010000766.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2292741"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2361)
                     /locus_tag="AC16CY_17630"
     CDS             complement(<1..>2361)
                     /locus_tag="AC16CY_17630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355676.1"
                     /translation="AVLTLALGIGTNTTVFTILKGITLRPLPGVQAADELVVLLSASK
                     TGERWPMNYRDYTDVRDGNTTLAGVAGTFPVAMTIGRGEDAERVWGEIVSGNLFRVLG
                     VSPLLGRLITEDDDRPPRGQPVAVISYDLWQRKFNGDPGVIGRPLQVGTRLLTIVGVT
                     PREYRGSMVGLGLHVFVPLRMQPDLLPMGEVLDRVERDNHWLMTVGRVKPGVSFDRAR
                     AEVKVLSDQITRDPTAAGVGEAALLVPLRESPYGAQAVMLPVFTVSMIMVGLVLLVAC
                     ANLSNILLARATVRRQEMAVRLAIGASRWRVIRLLLTESILLSLLGGAAALLLSLWAS
                     QYFSGVEIPLQFPVVVNAQVDGLVLLFTTLAAALCGVVFGLAPAWQSSHIALAPALVD
                     ARSTLQFRRSWLRSGLLVAQVAVSLALLVAAALVVRSQTEVARANPGFDPAGVSLFAM
                     DLSQSGYDSQTGPALYRNLLREVSGATGVRSASLASQLPLLVVGMVSRGVEPEGYTPA
                     PREDLNFGFNLVSEDYFTTMRIPLVSGRTFEPTDDDSAPKVAIVNQPFARKYWPGRDA
                     IGRTIEVGGEQHRVVGIVREIKYVNLTEEPRPYVYLPLSQMYTPQVTLHVRADGDAAA
                     AQNGVRAAVRRVDSALPLFDVRTLQAQADASLGTFVVATLFLSAAGVQALLLAAIGIY
                     GVVAFSVAQRTREIGIRIALGATPSRVLRLIMGQGLALSIAGLGLGVAIALASTRVLA
                     GMLYGVGPRDPLTIVATAAGLLGVALLACWIPARRALRVSATTALRY"
BASE COUNT          402 a          833 c          815 g          311 t
ORIGIN      
        1 gtatcgcagc gccgtcgtcg cgctgactct cagcgcccgg cgcgcgggga tccagcaggc
       61 gaggagcgcg acgccgagca ggcccgcagc cgtagcgacg atcgtcagcg gatcgcgcgg
      121 gccgacgcca tacagcatgc cggcgagtac gcgcgtggac gccagtgcga tcgccacgcc
      181 cagcccgagg ccggcgatcg acagcgcaag accctgaccc atgatgaggc gcagcacccg
      241 cgacggtgtc gcgccgagcg cgatacgaat cccgatttcg cgtgtgcgct gcgccaccga
      301 gaacgcgacg acgccgtaga tgccgatggc cgcgagcagc agcgcctgca cgccggcggc
      361 gctgagaaac agcgtggcca cgacgaaggt gccgagcgag gcgtccgcct gggcctgcag
      421 ggtgcgcacg tcgaagagcg gcagcgcgct gtccacgcga cggacggccg cccgcacgcc
      481 gttctgcgcg gccgctgcgt ccccgtccgc acggacgtgc agcgtgacct gcggcgtgta
      541 catctgcgag agcggcaggt acacgtacgg acgaggctcc tcggtgaggt tgacgtactt
      601 gatctcgcgc acgatcccga ccacgcgatg ctgctcgccg ccgacctcga tggtccggcc
      661 gatcgcatcc cgccccggcc agtacttcct ggcaaaaggc tgattgacga tcgccacctt
      721 cggcgccgag tcgtcgtcgg tcggctcgaa cgtgcggccg ctcacgagcg ggatgcgcat
      781 ggtcgtgaag tagtcctccg agacgaggtt gaagccgaag ttcaagtctt cgcgcggggc
      841 gggagtgtag ccttcgggtt cgacgccgcg cgacaccatc ccgacgacca gcagcgggag
      901 ttgagacgcg aggcttgccg atctcacgcc ggtcgccccc gagacctccc gaagcaggtt
      961 cctgtacagg gcgggtccgg tctgcgagtc gtagccgctc tgcgacaggt ccatggcgaa
     1021 caggctcaca ccggcgggat cgaacccggg gttcgcgcgc gccacctcgg tctggcttcg
     1081 aacgacgagc gccgcggcca cgagcagggc gagagacacg gccacctggg cgaccaggag
     1141 gcccgaacgc agccacgatc ggcggaactg cagcgtcgat ctcgcgtcca cgagcgcggg
     1201 cgccagggcg atgtgcgacg actgccaggc cggcgccagg ccgaacacca ccccgcagag
     1261 ggcggccgca agcgtcgtga acagcagtac caggccgtcc acctgagcgt tcacgaccac
     1321 cgggaactgc agcgggatct cgacgccgct gaagtactgg ctcgcccaca ggctgaggag
     1381 cagggccgcg gctcctccca gcagcgagag caggatcgat tcggtgagca gcaaccggat
     1441 cacgcgccag cggcttgcgc cgatggcgag gcgaaccgcc atctcctggc gtcgcaccgt
     1501 cgcgcgcgcg agcaggatgt tcgacaggtt ggcgcaggcc accagcagca cgagacccac
     1561 catgatcatc gacaccgtga acacggggag catcaccgcc tgcgcaccgt acggtgactc
     1621 cctgagcgga acgaggaggg cggcctcgcc gacgccggcg gccgtgggat cgcgcgtgat
     1681 ctggtcgctc aggaccttca cctcggcccg cgcccgatcg aagctcacgc cgggcttgac
     1741 ccggccgacg gtcatcagcc agtgattgtc gcgctcgacg cgatcgagca cctcgcccat
     1801 cggcagcaga tccggctgca tccgcagcgg cacgaacacg tgcagtccga gtccgaccat
     1861 cgagccgcga tactcccgcg gtgtcacgcc gacgatcgtg agcagccgcg tgccgacctg
     1921 cagcggccgg ccgatgacgc cgggatcgcc gttgaatttc cgctgccaca ggtcgtagct
     1981 gatcacggcg acgggctgtc cgcgcggcgg tcgatcgtcg tcttcggtga tcagccggcc
     2041 gagcaggggg ctcaccccga gcacccgaaa gagattcccg gagacgatct caccccacac
     2101 ccgctcggcg tcctcgccgc ggccgatcgt catcgccacc ggaaacgtgc cggcgacgcc
     2161 cgccagcgtg gtgttgccgt cgcgcacgtc ggtgtagtcg cgatagttca tcggccagcg
     2221 ctcgcccgtc ttcgacgcgg acaggagcac gacgagctcg tcggccgcct ggacgcccgg
     2281 cagcgggcgc agggtgatgc ccttcagtat cgtgaagacc gtggtgttgg tgccgatgcc
     2341 gagcgccagc gtgaggaccg c
//
LOCUS       DBNGSH010000767         1561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4499120, whole genome shotgun sequence.
ACCESSION   DBNGSH010000767 DBNGSH010000000
VERSION     DBNGSH010000767.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4499120"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="AC16CY_17635"
     CDS             complement(<1..413)
                     /locus_tag="AC16CY_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355677.1"
                     /translation="MTSGTLSAVCGVAASIAMCLPASAQSSRIGGGNISRPTFGFYWV
                     THLEPSTPGVAESFGTLTLDGPKSIDRLILDRAKQMYFGYEVLVEPLGKHSFRLTFQP
                     LLMTQDLKSRMTAQRLELHDPASPWTLLPSPSFPKP"
     gene            complement(410..721)
                     /locus_tag="AC16CY_17640"
     CDS             complement(410..721)
                     /locus_tag="AC16CY_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355678.1"
                     /translation="MEPLDDKDLSELLSRWDAPGAPRGLWRRINSRRGSWWTWLLTGS
                     IRVPVPVGAAIVILAAAWIYSTPSPDLATPTPPPQAPSVSLADFQPVVKLEPRIIKVQ
                     Q"
     gene            complement(705..1226)
                     /locus_tag="AC16CY_17645"
     CDS             complement(705..1226)
                     /locus_tag="AC16CY_17645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3355679.1"
                     /translation="MTDEEFRAAFDQHKDAIYRFAWRMTGNDAADDIAQDVFLVLWRQ
                     PDRFDPARGSLRAYLLGVARNLTRKRWRDAHRWEEIDEERFVAQPVDVERRETAQIVA
                     DAIRALPPLQREALVLAEYEQLSLEDIARIADAEVGTVKSRLHRARENLRRMLAPLKP
                     ASAVRSTRYGTAG"
     gene            1273..>1561
                     /locus_tag="AC16CY_17650"
     CDS             1273..>1561
                     /locus_tag="AC16CY_17650"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR3355680.1"
                     /translation="MTRLGAVLVSAFCLTACAGSSQRPQAALVERSHVSMGSEVRLTA
                     WAPDDAATLAAFAKVFDEFDRLDRLMSVWREGSDVLRINAGAGGPAVPVSPE"
BASE COUNT          260 a          525 c          514 g          262 t
ORIGIN      
        1 ggcttgggaa acgagggcga cggcagcagc gtccatggcg acgcgggatc gtgcagctcc
       61 aatcgctgcg cggtcattcg tgacttcaag tcctgggtca tgagaagcgg ctgaaaggtc
      121 aaccggaagc tgtgcttccc gagcggttcg acgagcactt cgtacccgaa gtacatctgt
      181 ttcgcgcgat ccaggatgag gcgatcgatc gactttggtc catcgagcgt cagcgtgccg
      241 aagctctcgg ccacgcccgg cgtcgagggc tccaagtgcg tgacccaata gaacccgaac
      301 gtcggcctgg agatgttgcc gccgccgatt cggctggact gagccgacgc cggcagacac
      361 atcgcgatcg atgcggccac gccgcacacg gcactgagcg ttccgcttgt cattgctgca
      421 ccttgatgat tctcggttcg agcttcacga ccggctgaaa gtctgccagc gacaccgacg
      481 gcgcctgcgg cggcggcgtg ggagtggcga gatcgggcga cggggtggaa taaatccagg
      541 ccgccgcgag gatcacgatg gcggcaccga ccgggacggg cacacggatc gaccccgtca
      601 gcagccacgt ccaccacgat ccgcgccgtg agttgatccg gcgccacagg cctcgaggtg
      661 cgcccggcgc gtcccatcgg ctcagcagct cgctcaggtc cttgtcatcc agcggttcca
      721 taacgtgttg acctcactgc gctcgccggc ttcagcggag cgagcattcg tcgcaggttc
      781 tcgcgcgccc gatgcaggcg cgacttcacg gtcccgactt cggcatcggc gatgcgggcg
      841 atgtcctcga gcgacaactg ctcgtattcg gccaggacga gcgcctcacg ctgcagcggc
      901 ggcagcgccc ggatggcgtc ggcgacgatc tgcgcggtct cgcgccgttc cacgtcaacc
      961 ggctgcgcga cgaagcgctc ctcgtcgatc tcctcccatc gatgcgcatc ccgccatcgc
     1021 tttcgcgtca ggttgcgcgc cacgcccagc agatacgcgc gaaggctgcc gcgggcggga
     1081 tcgaagcggt ccggctgccg ccacaggacg agaaatacgt cctgagcgat gtcatccgcc
     1141 gcgtcgttcc cggtcatccg ccaggcgaag cggtagatcg cgtccttgtg ctggtcgaac
     1201 gcggcccgga actcctcgtc tgtcactcga tcctgattac cagaaagggg gccaaaggat
     1261 acgctagctt ctatgactcg attgggggcc gtcctggtct ccgcgttttg cctgacggcc
     1321 tgcgccgggt cgagccagcg tccgcaggca gcgctcgtcg aacgatccca cgtcagcatg
     1381 ggatcggagg tccgcctcac cgcgtgggcc cccgacgacg ccgcgaccct ggccgccttc
     1441 gcgaaggtct tcgacgaatt cgatcgcctc gaccggctga tgagcgtctg gcgcgaggga
     1501 agcgacgtgc ttcgcatcaa cgcgggcgcg ggaggacccg ccgtgccagt gagccccgag
     1561 g
//
LOCUS       DBNGSH010000768         3987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4670258, whole genome shotgun sequence.
ACCESSION   DBNGSH010000768 DBNGSH010000000
VERSION     DBNGSH010000768.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4670258"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="AC16CY_17655"
     CDS             <1..1782
                     /locus_tag="AC16CY_17655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR3355681.1"
                     /translation="GGRFERVSWDDALNLIARHMREASETWGGESILPYCYGGSNGLL
                     TQDTGDATLFRRLGASRLARTVCAAPTGAANGALYGKMPSVTYEDYPEARLIIVWGCN
                     PSASGIHMVPYIREAQRRGARLVVVDPRTTPLAKQADLHLAVRPGTDLPIALSIHRHL
                     FESGFADRTFIDAHTRGAERLREKALPWTFERAAAESGVPVAQLETLADWYARTTPAL
                     IRCGWGQERNRNGGSSSLAILALPAVGGKFCVRGGGYAMSNTAAWGIERTWIGAAEPA
                     TRVINMNRLGRALLEADPPVKVLFVYNSNALVTSPDQRRILSGLEREDLFTVVFDQVM
                     TDTAHYADVLLPATTFLEGYDIARAYGPIGLRLAKPVIEVVGEARSNADVFGDLSRRL
                     GVAEEDDPNGEIEEMLDVLSKLPGAIGEELREYGAAVPLYGGRPVQFVDVMPRTADGK
                     VDLFPEALEREAPAGLYAYQPDPSTTEFPLALISPASERTISSTLSELPRPAVRLSMH
                     PDDAAARDLKDGDLVRVYNALGEVRCPLQVGTWIRPGTVSLPKGLWRRHTANGYTTNA
                     LVPDTLTDLGAGACFNDARVQVEAGSH"
     gene            complement(1914..3428)
                     /locus_tag="AC16CY_17660"
     CDS             complement(1914..3428)
                     /locus_tag="AC16CY_17660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3355682.1"
                     /translation="MGQIGITTAVGLGDRVELFGSWRVVRLRRNVSPVFFPGDADHGG
                     VINDFPFQDEHWSETLGEPITVGAKFDILSQNRADGIALAPRVMVKFPSGGATRASTR
                     AFAADFGIVLSSEISEKFEWAGTAGYIARSSPGAEGPVTPFSSLDNGFELSSGFKWGV
                     GTSFPSRNPFRVLLELEGEFPTGDSVTVVNPPLIAQDGSLAPVVSNTASHTNFKGGIV
                     WQHSGGFFLHTGANYTFGAGSQTVGGIPWDHSAWGWDVRLGFHPGVPPPRQLTLVKPP
                     PPPPPPPPPPPPPPAPKPPAPAANRNPSFNIVARCEPTSVRPGQTVKVSSQATDPDGD
                     ALTYQWTAPQGTFNPANAANSTWTAPNQPGNVTLTVTATDNRGGKATSSCNVQVAQRQ
                     VIMFEDVHFAFDRFNLSPEGIKILDGTVATLQANPDVNVTIEGHTDSIGTSEYNLALG
                     ERRANSVRDYLTGRGIAGNRIRTVSYGEERPIATNDTAAGRAMNRRAHTAMVIE"
     gene            complement(3749..3973)
                     /locus_tag="AC16CY_17665"
     CDS             complement(3749..3973)
                     /locus_tag="AC16CY_17665"
                     /inference="COORDINATES: protein motif:HMM:NF022150.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HIR3355683.1"
                     /translation="MSREKQREIASKGGRAAHEKGTAHEWTPDEARAAGRKGGQSSRG
                     GRGRLFPNASSEVHNLTDATGTGREPGRVD"
BASE COUNT          648 a         1328 c         1321 g          690 t
ORIGIN      
        1 ggggggcgct tcgagcgagt ctcgtgggat gacgcgctca acctgatcgc gcggcacatg
       61 cgcgaggcaa gcgagacgtg gggaggcgag tcgatcctcc cctattgcta cggagggtcc
      121 aacggcctgc tgacgcagga cactggtgac gccacgctgt tccgccgcct cggcgcatcg
      181 cggctcgcgc gcacggtctg cgccgctccg accggcgcgg ccaacggcgc gctctacggc
      241 aagatgccgt cggtgaccta cgaggattat ccggaagcgc gcctgatcat cgtctggggg
      301 tgcaatccct cggcgtcggg catccacatg gtgccctaca tccgcgaagc ccagcgccgt
      361 ggcgccaggc tcgtggtcgt cgacccccga accacaccgc tcgccaagca ggccgacctt
      421 catctggcgg tccgacccgg caccgacctg ccgatcgcac tgtcgatcca ccggcatctc
      481 ttcgaaagcg gcttcgccga tcgcacgttc atcgacgcgc acacgcgcgg tgcggagcgc
      541 ctgcgcgaga aggcgctgcc gtggaccttc gagcgcgcgg ccgccgagtc gggcgttccg
      601 gtcgcgcagc tcgagaccct ggcggactgg tacgcgcgga cgacccccgc gctcatccgc
      661 tgcggctggg ggcaggagcg caatcgcaac ggcggcagct cgagcctcgc catactggcg
      721 ctgcccgccg tcggcgggaa gttctgcgtg aggggcggcg ggtatgccat gagcaacacc
      781 gcggcgtggg gcatcgaacg gacatggatc ggagcggcgg agccggcaac gcgcgtcatc
      841 aacatgaatc gcctgggacg ggcgctgctc gaagccgacc cgcccgtgaa ggtgctgttc
      901 gtctacaact cgaacgccct ggtgacctcg cccgaccagc gccggatcct gagcggcctc
      961 gagcgcgagg acctcttcac ggtcgtcttc gatcaggtga tgaccgatac ggcccactac
     1021 gccgacgtgc tcctgccggc cacgacgttc ctcgaaggct atgacatcgc cagagcgtac
     1081 ggaccgatcg gtctgcgcct ggccaagccg gtgatcgaag tcgtgggaga ggccaggtcg
     1141 aacgcggacg tcttcgggga tctctcgcgg cggctcggcg tggcggaaga agacgacccc
     1201 aacggcgaga tcgaggagat gctggacgtc ctctcgaagc tgccgggggc aattggagaa
     1261 gagctccggg aatatggagc cgccgttccg ctgtacggcg gacggccggt acagttcgtg
     1321 gacgtcatgc cgcgaaccgc cgatggcaaa gtcgatctct ttcccgaggc gctcgagcgg
     1381 gaggcgcccg ccgggctgta tgcgtatcag cccgatccct cgacaacgga gttcccgctg
     1441 gcgctgatct cgcccgcgag cgagcgcacg atcagctcta cgctgtcgga gctcccacgg
     1501 ccggccgtgc ggctgtcgat gcaccctgac gacgccgcgg cgcgggatct gaaggacggc
     1561 gacctcgtcc gcgtctacaa cgccctcggc gaagtgcgct gccctcttca ggtgggaacc
     1621 tggattcgtc ccggaaccgt ttcgctgcca aaggggttgt ggcgccgtca tacggcgaac
     1681 ggctacacca cgaacgcgct ggtccccgac acattgaccg accttggcgc cggtgcgtgc
     1741 ttcaacgacg cccgcgtaca agtcgaagcc ggcagccact agcagatcct gctaacagct
     1801 gccggcttct gattgcccag gcgggtctgc cttcgacttc gctcaggcca ggaaagaccc
     1861 gcctctactg agtgcagtgc ggaggccgtg cccccgcact gcgcgtcagt cgcttactcg
     1921 atgaccatcg cggtgtgggc gcggcgattc atcgcccggc ccgccgccgt gtcgttggtc
     1981 gcgatcggac gttcctcccc gtagctgacc gtccggatcc ggttgccggc gattccccgg
     2041 cccgtgaggt agtcgcgcac gctgttcgcg cgccgttccc cgagcgccag gttgtattcc
     2101 gacgtcccga tgctgtcggt atggccctcg atcgtcacgt tgacgtcggg gttggcctgc
     2161 agcgtcgcaa cggtgccgtc cagaatcttt atgccctcgg gcgacaggtt gaagcggtcg
     2221 aacgcgaagt gcacgtcctc gaacatgatg acctggcgct gcgccacctg cacgttgcag
     2281 gaggaggtcg ccttgccacc gcgattatcc gtggccgtca ctgtgagcgt gacgttcccc
     2341 ggctgatttg gcgccgtcca ggtcgagttc gcggcgttcg cggggttgaa ggtgccctgc
     2401 ggagccgtcc actgataggt caaggcgtcg ccatcgggat cggtcgcttg cgagctcacc
     2461 ttcaccgtct gacccggccg aacgctggtc ggctcgcacc gcgccacgat gttgaacgac
     2521 ggattgcgat tggccgcggg cgccggcggc ttcggcgctg gtggcggtgg tggcggcggc
     2581 ggtggcggcg gcggcggtgg cggcggcttg acgagcgtca actgacgcgg cggcggcacg
     2641 ccagggtgga agcccaggcg gacgtcccat ccccacgcgg agtgatccca tggaatacca
     2701 ccgacggttt gcgagcccgc gccgaaggtg tagttcgctc cggtgtgcag gaagaacccg
     2761 cccgaatgct gccaaacgat tccgcccttg aagttggtat ggctcgccgt gttgctgacg
     2821 acgggcgcga ggctcccgtc ttgcgcgatg agcggagggt tgaccaccgt gaccgagtcg
     2881 cccgtcggga actcgccttc gagctcgagg agcactcgga acgggtttcg gctgggaaac
     2941 gacgtgccca ctccccactt gaagccgctg gacagctcga aaccgttgtc aagcgaggag
     3001 aagggcgtca ccggtccctc ggccccaggg ctgctgcgag caatgtagcc agccgttccg
     3061 gcccattcga acttctcgct gatttcgctg ctgaggacga tcccgaagtc cgcggcgaaa
     3121 gctctcgtgc tcgccctcgt cgctccgccg ctcgggaact tcaccatcac gcgcggcgca
     3181 agcgcgatcc cgtccgcccg attctgggac aagatgtcga acttcgcgcc gacggtgatc
     3241 ggctcgccga gcgtctcgga ccaatgctcg tcctggaacg gaaagtcatt gatcacgcca
     3301 ccgtggtcgg catcgccagg aaagaagacc ggcgagacgt ttcggcggag acgaaccacg
     3361 cgccacgatc cgaaaagctc gacgcgatct ccgagtccga cagcagtcgt gattccgatc
     3421 tgacccacgt cggtcaggcc ttgcagtcgt tcagactccg ctctgaagag actgaaggac
     3481 catttgcctg cgggcagcgt ttcggcgatc gggacgaccc aaaatcccgt gtcgccgaag
     3541 aatgtcggca acgccgggcg aggggtgttg tcgcccgtca actcgtattg ctctttcgcc
     3601 gactgggagg ccgaactttg tggagccgcc gcctgtgccg cgacggtccc gacaaagagc
     3661 gcagctaaaa gtagactgga cagaaatgta cgcaagttga tctcccgtca tcctccggcg
     3721 gcgacgtatg gccgacgcgg ctagcttttt aatcgacgcg gccaggctcc cggccggtgc
     3781 cggtagcatc cgtcagatta tgcacctcag aagaagcatt tggaaacagc cgacctcggc
     3841 cacctctgct gctctgtcct ccttttcggc cagcggcgcg ggcctcatca ggggtccact
     3901 cgtgggcggt tcccttttca tgtgcagctc ggccgccctt gctggcgatt tcgcgttgct
     3961 tttcgcggct cattgacgcg aatcctc
//
LOCUS       DBNGSH010000769         5738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_255161, whole genome shotgun sequence.
ACCESSION   DBNGSH010000769 DBNGSH010000000
VERSION     DBNGSH010000769.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_255161"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..490
                     /locus_tag="AC16CY_17670"
     CDS             155..490
                     /locus_tag="AC16CY_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3355684.1"
                     /translation="MPRPPIPDLLPGTLDLLILRTLQTAPLHGWAISDRIQQVSQDVL
                     QVNQGSLYPALHRLEHHGWIDAEWRVSELGRRAKYYRLTAAGRRQVAIELNAWERMSA
                     AIGRVVKLA"
     gene            496..3117
                     /locus_tag="AC16CY_17675"
     CDS             496..3117
                     /locus_tag="AC16CY_17675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355685.1"
                     /translation="MTLRDLVLRLRALLFPRRVERELDEELAFHVERETQKHVARGVS
                     PADARREAHTRFGPVPLAADECRDARGTAGVDDLVRDILYAFRTFRRAPLVALTIVAT
                     VALGLGLVTVAFTFYNVGFLRVDAVRSPDELFAVERPTAPGAETLRRFTRREYDAMRR
                     ETNVFTDVFATIRAVRVRIDGRTASSALVTGNFFRALGVSAVLGRSLTPDDDEPGAAR
                     PVIALSHRGWLRLFAGDPGVVGRGVRINGLPYDVVGVMPEDFRGLAIGPPDYWAPLGL
                     VSRFREAPAGGADEIAVEVVGRLKQGTSLEVAADALTAWEARRADPTMSLRNPVSIAL
                     EPRQGTLSEDVIELVTVSVPLVFAFGLILLVGCANVANLQLARGVSRQREIGIRLSLG
                     ASRRRVIRQLLTESLLLALMAAVCGLGLSRVIQEAAVYAITATMPAELAGELSFDVLP
                     SDWRVMVFLVVGAVIATLFFGLAPALQSTRIDLVRSMRGEGTRDARPGRARYVLIAFQ
                     VGASALLLVSASVLLRSVMATVDVDPGVRTSDTLMVMVTNEPRRAALLRAVATHPLVA
                     VVAASSPISSAAVVATGTSRHATVAIAASPDYFDVLGLEIVNGRRFTPDERTSDSGVA
                     VVTETLARELSPAGDVVGQSVRLDASGPAGASSRPLTVVGVMRDVKGPLSLESFPLRS
                     VYVPTRPESAGTSLTLRIRGDPDRARQVLVDDLIGVDPGLGQILTMRNLARFPTYVLS
                     IAFWVAVVLGGLALILTASGLFSVLSYLVEQRAKDIGVHMALGATMRDAAALVLSTLL
                     RPVCIGLAIGVALAAAVATMLMAVSDGEVGTLIKVFDPTAYTTGVLVIVVSCALAATV
                     PALRAARIDPIATLRRE"
     gene            3159..4103
                     /locus_tag="AC16CY_17680"
     CDS             3159..4103
                     /locus_tag="AC16CY_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355686.1"
                     /translation="MLGRSNRFGMSRRAFLAAGATAGIGGAAWLSVSSSWGARFIIPP
                     APHKPAPATWSDNAVTLAWLGHATVLINFYGVRILTDPALYSRIGVDLGIGSLGPLRL
                     VRCALTPDELPDIDLVLVSHAHFDHLDTPSLGAIRGMPAALMGAGTSDLLPRRPYSSI
                     RELRWNEAATVTTPHGDLFVRAIEVKHWGARVGRDTWRGYTGWIVEREGRRLLIGGDT
                     AVTSVFNSHRRFGPFDAAVMPIGAYNPYIHYHCTPEQAVAMADAAGARAIVPVHHQSF
                     QLSHEPFMEPIERIQEALVSEHDRLALREIGETVVVRS"
     gene            4267..4482
                     /locus_tag="AC16CY_17685"
     CDS             4267..4482
                     /locus_tag="AC16CY_17685"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3355687.1"
                     /translation="MSDHHSQALTGHEVAKQLGLSVATLRAWRLKSKGPRFVRFGRAV
                     RYLAADVEHFVEASVVDQSKHVGDANL"
     gene            4501..4959
                     /locus_tag="AC16CY_17690"
     CDS             4501..4959
                     /locus_tag="AC16CY_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355688.1"
                     /translation="MTLWKRGRQYWMDIVVGGQRYREPLHATDWREAKEHEKERVAEL
                     ARRPADPAGRGRSYGALDVSKAIEAYAKERRAQVSPRMVAYWTENAKPLGEFFGTTTL
                     PTSHRNRSRHIRTRGSTPVARRRQSMANSQSCGRSCATHDSGIASKKTAR"
     gene            4911..5309
                     /locus_tag="AC16CY_17695"
     CDS             4911..5309
                     /locus_tag="AC16CY_17695"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3355689.1"
                     /translation="MRHARLWYRLQEDRALKNTKPPVGQALTDDEQKRLFEAARSRPE
                     WIFAYVASALAFYCGLRACEIRGLQWKHVDLDRARIEIRRSKTPAGWRDPSLNDACAR
                     ALKELYSRAKAVGFDDGESAARAEARALRR"
BASE COUNT          937 a         1813 c         1923 g         1065 t
ORIGIN      
        1 gtgttcatgc tggtcccgaa gggcagtccg ttggtgttgc ccttgtagac ccgacactgc
       61 tgtctctctc acggccgcgc cgccgttgtt gacaccaatt aggcttggtg ttgacagacg
      121 acaccaatcg ccgttagtgt cgtatgtcga catcatgcct cgccccccga ttcccgatct
      181 attgccaggg accctcgacc tgctcatcct ccgtacgctg cagaccgctc cgttgcacgg
      241 ctgggcgatc tccgatcgca tccagcaggt gtcgcaggac gtgctgcagg tcaatcaggg
      301 ctcgctctat ccggcgctcc atcgtctcga gcaccacggc tggatcgacg ccgagtggcg
      361 cgtgtccgag ttgggccgcc gggcaaaata ctatcggctc accgcggcag gccgccggca
      421 agtggcgatt gagctcaacg cgtgggaacg aatgtcggca gccatcggcc gcgtcgtgaa
      481 gctggcgtga gacggatgac gcttcgcgat ctggtcttgc gtcttcgcgc gctgctgttt
      541 ccgcgccgcg tcgaacgaga gctcgacgag gagctcgcgt ttcacgtcga gcgcgagacg
      601 cagaagcacg tcgcacgcgg cgtgagtccc gccgatgccc ggcgcgaagc gcacacgcgc
      661 ttcggccccg tgccgttggc ggcagatgag tgtcgcgatg cccgaggtac cgcaggcgtt
      721 gacgacctcg tgcgcgacat cctctatgcc tttcgcacgt tccggcgcgc gccgcttgtc
      781 gcgctcacga tcgttgcaac ggtggccctc ggcctcggcc tggtcaccgt cgcgttcacg
      841 ttttacaacg tcgggttcct tcgcgtcgat gcggtgcgga gtccggacga gttgttcgcc
      901 gtggagcggc cgacggcccc aggcgcggag acattgcgcc gattcacgcg ccgcgaatat
      961 gacgcgatgc gtcgagaaac gaacgtcttc actgatgtct tcgcgacgat tcgcgcggtg
     1021 cgagtccgaa tcgatggccg tactgcgagc tctgcgctcg tgactgggaa ctttttccgg
     1081 gcgctcggcg tctcggccgt gctcgggcgt tcgttgacgc ctgatgacga cgaacccggt
     1141 gcggcacggc cggtgatcgc attgagtcac cgcggctggc tcaggctgtt cgccggcgat
     1201 ccgggagtcg tcggccgcgg cgttcgcatc aacgggctgc catacgacgt cgtcggcgtc
     1261 atgcccgaag actttcgtgg gctggcgatc ggcccgccgg actactgggc accgctgggt
     1321 ctcgtctcac gattccgcga ggctcctgcg ggcggagccg acgagatcgc cgtagaggtc
     1381 gtggggcgat tgaagcaggg gacatcgctc gaggtagcgg cggacgcgct cactgcatgg
     1441 gaggcacgac gggcggatcc gactatgtcg ttgcgcaatc cggtctcgat cgcgctcgag
     1501 ccgcgtcaag gcacgctgtc ggaggatgtc atcgagctcg tcaccgtctc agtacctctc
     1561 gtcttcgcgt tcggattgat tctcctggtc ggctgcgcga acgtcgccaa cttgcagttg
     1621 gcccgcggtg tgtcgcgcca gcgggaaatc gggatcaggc tgtcgctcgg cgcgtctcgg
     1681 cggcgtgtga tccgtcaact gttgacggag agtctcctcc tcgcgctcat ggcagcggtc
     1741 tgcggtctcg gcctgtcgcg cgtgattcag gaggccgcgg tctacgccat caccgcgacg
     1801 atgcccgcgg agctcgccgg cgagctcagt ttcgatgtgc tgccgtcgga ctggcgggtg
     1861 atggtattcc tcgtcgtcgg cgccgtgatc gcgacgttgt tcttcggcct ggcgccggcg
     1921 ctccagtcaa cgcgtatcga tctcgtgcgc tcgatgcgcg gcgaggggac gagagacgcg
     1981 cgtccagggc gcgcgcgata tgtgcttatc gccttccaag ttggcgcatc ggcgctcctg
     2041 ttggtatctg cgagtgttct gctgcggagc gtcatggcga ctgtggacgt cgatcccggt
     2101 gtgcggacga gcgacacact gatggtgatg gtcaccaatg agccacggcg cgcggcattg
     2161 cttcgggcgg tggcgaccca tccgctcgtc gccgtggtgg ccgcgtcgtc gccgatatcc
     2221 tccgcggcag tggtcgcaac agggacatcg aggcacgcga cggtggcgat cgccgcgtcg
     2281 ccagactact tcgacgtgct cggtctcgag atcgtgaacg gccggcgttt cacgccagac
     2341 gaacgcacca gcgattcagg agtcgccgtg gtgaccgaga ccctggcgcg cgagctctcg
     2401 ccagcgggtg atgtggtagg gcagtccgtg cgtctcgatg cgtcaggccc tgcgggagcg
     2461 tcatcgcgac cgctcacagt ggtcggtgta atgcgcgacg tgaagggtcc gctctcgctg
     2521 gagtcctttc cgttgcggag tgtgtacgtg cccaccaggc ccgagagtgc tgggacctcg
     2581 ttgacgctgc gcattcgcgg cgatcccgac cgggcccggc aggtactggt cgacgacctg
     2641 atcggtgtgg accccgggct gggccaaatt ttgacgatgc ggaatcttgc tcgctttccg
     2701 acctacgtat tgagtatcgc gttctgggtg gctgtcgtcc ttggcgggct ggcactcatc
     2761 ctcacggcgt cgggcctgtt cagcgtgctg tcatacctcg tcgagcagcg ggcgaaggac
     2821 atcggtgtgc acatggcgct tggcgcaacc atgcgggacg ccgcggcgtt ggtgctctcg
     2881 acgctgctgc gcccggtgtg tatcggtctc gctatcggcg tcgcgctggc tgcggcggtc
     2941 gcgacgatgc tgatggcagt gagcgacgga gaggtcggaa ccctgatcaa ggtcttcgat
     3001 ccgaccgcgt acacgacggg cgtgttggtc atcgtcgtct catgtgcgct ggctgcgaca
     3061 gtcccggcat tgcgcgccgc ccgcatcgat ccgatcgcga cgctcaggcg tgagtgaacg
     3121 ttttgctttc gtcacccatg ccgtcagact gtacgcgcgt gctcggaaga tcgaatcgtt
     3181 tcggcatgag ccggcgcgcg ttcctggcag cgggagcgac ggccggcatc ggtggcgcgg
     3241 cctggctgag cgtgtcatcg tcatggggcg ccaggttcat cattcctccc gcaccacata
     3301 agccggcccc ggcaacctgg tccgacaacg cggtgacgct cgcgtggctc ggacacgcga
     3361 cggtgctgat caacttctac ggtgtgcgca tcctgacgga ccctgcgctg tattcgcgca
     3421 tcggcgtgga tctcggcatt ggctcgctgg gcccgctgcg cctcgtccgg tgtgcgctta
     3481 cgccggacga actccccgac atcgacctcg ttctcgtgtc gcacgcacac ttcgatcatc
     3541 tcgacacgcc atcgctcgga gcgatccgcg gaatgccggc ggcattgatg ggggccggca
     3601 cgtcagacct tctgccccgc cgcccctatt cgtcgatccg cgagcttcgc tggaacgagg
     3661 ccgcaaccgt cacgacgccg cacggcgatc tgttcgttcg cgcaatcgag gtcaagcact
     3721 ggggtgcgcg tgttgggcgc gacacgtggc gtgggtacac cggatggatc gtcgaacgcg
     3781 aaggccgacg tctcctgatc ggtggagaca cggcggtcac gtcggtgttc aacagtcatc
     3841 gccgctttgg tccgttcgac gccgccgtga tgccgattgg cgcctacaac ccctacatcc
     3901 actaccactg cacgcccgaa caggcagtcg ccatggcgga cgccgcgggc gcccgtgcga
     3961 tcgtgcccgt ccaccatcaa tcgtttcaac tgagccacga gcccttcatg gaaccgatcg
     4021 aacggattca ggaagcgctc gtcagcgagc atgaccggct cgcgctgcgc gagatcggcg
     4081 agacggtcgt cgtgcggagc tgacggcacc tcgatgaggc acagtcgccg cgacgtgctg
     4141 tcgagatgcg ccgcgcggcg atcgagaggc gcgaccttga caccgcttcc gcgcgcccgg
     4201 tgaacggcaa ttgtcggagc gagccgaatc acgaaatgcg aatacgagca gaacgcgagg
     4261 aatcacatga gcgatcatca ctcgcaagcc ttgaccgggc acgaagtagc gaagcaactc
     4321 ggtctgtcag tggccacgct gcgggcgtgg cgactgaaga gtaaagggcc acgattcgtc
     4381 cgcttcgggc gcgccgtgcg ctacctggca gccgacgtcg aacacttcgt cgaggccagt
     4441 gtggttgacc agtcgaagca cgttggcgac gctaacctgt gatagaagac gacgacgtcg
     4501 atgaccctgt ggaagcgcgg ccgacagtac tggatggaca tcgttgtggg cggtcaacgg
     4561 taccgagagc cgctccacgc gacggactgg cgcgaggcca aagagcacga gaaggagcgc
     4621 gtggccgagc tcgcgcgtcg gcccgcagat ccggcaggtc gaggtcgctc gtacggcgcg
     4681 ctcgatgtca gcaaagcgat cgaggcgtac gcgaaggaac gccgcgccca ggtgtcaccg
     4741 cgcatggtgg cctactggac cgagaacgcg aagccgcttg gcgagttctt cggcacgacg
     4801 acgctcccca cgtcacaccg gaacagatca cggcatatca gaacgcgagg atcgacgccg
     4861 gtcgcgcgcc gaagacagtc aatggcgaac tctcagtcct gcggcaggtc ttgcgccacg
     4921 cacgactctg gtatcgcctc caagaagacc gcgcgctgaa gaacacgaaa ccgcctgtag
     4981 gccaggcgct gaccgatgac gagcagaagc gtctattcga ggcggcgaga tcgaggcctg
     5041 agtggatctt cgcgtacgtt gcctcggcac tcgccttcta ctgtggcttg cgcgcgtgcg
     5101 agatccgcgg cctgcagtgg aagcacgtcg atttggacag ggcgcgcatc gagatccgac
     5161 gctcgaagac gccggcagga tggcgcgacc cgagcctcaa cgacgcttgt gcgcgtgcac
     5221 tcaaggagct ctactcgcgg gccaaggccg tgggctttga cgacggtgag tcagccgcgc
     5281 gggctgaagc ccgcgctcta cgtcgatgaa gcgaagaagc cgacgtcgca cgatcgctgc
     5341 gacagggtta tgtcacagtt cacgtcacag tgggacgatc tgaagaagga gatcgcccgc
     5401 tggagccccg ccgaaggcgg ccgcccgagc ggagcgaggg ccagagatat agagccaaaa
     5461 acgcgagggg acaaggaaca agccccgagc ggttttggct cctcgggctg gactcgaacc
     5521 agcaaccctc cggttaacag attggtgcag gtcttgtatc tcgttgattc ttcgtgcctg
     5581 tagctcgggt gcgatcacgc tgttacccgg cgttcgggag caaattgtta accgattgtt
     5641 cactgtagcg tcgcgccggt cgcaatgaac attcccgagc cgactctgcg gccgcacgtt
     5701 cggcgctccg aggtcccctc cggcaaacag ttggctac
//
LOCUS       DBNGSH010000770         2015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1104542, whole genome shotgun sequence.
ACCESSION   DBNGSH010000770 DBNGSH010000000
VERSION     DBNGSH010000770.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1104542"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="AC16CY_17700"
     CDS             <1..1074
                     /locus_tag="AC16CY_17700"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3355690.1"
                     /translation="SHNVERRLQPARRDERPLINAAIVGMGWWGRTIVESVQADSNIV
                     RFVAGATRTASPEVKAFVDAQKLRLVDSYDALLADRSVDAVVLATPHSMHSSQVIAAA
                     AAGKHVFCEKPFALKKADAEAAVAATAKAGVTLGLGYNRRFHPEMTALRERIRTGALG
                     TVLHVEATMTFPNALQLKPEAWRAQRDETPCGGLTPMGVHAIDGMIDLCGEIAQVYCQ
                     SFHRVVPVDSDDTTSMLFRMKSGASGYLGTITATGPGFSFQVFGSNGWVRLEGMTHVA
                     GASSEERRTRLFSTCKFQPIKGPAEVWEAARLDVTRACLEAFANAASGGAPYLIPTSE
                     MIHGAAVTEAVVKSAGSGKVEQV"
     gene            1071..>2015
                     /gene="eboE"
                     /locus_tag="AC16CY_17705"
     CDS             1071..>2015
                     /gene="eboE"
                     /locus_tag="AC16CY_17705"
                     /inference="COORDINATES: protein motif:HMM:NF035939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HIR3355691.1"
                     /translation="MNLGKGLGHLTYSTLVHPTDNWDQLWKSVNTYLPAVKARMAPHE
                     KFGVCLRISAPSAAALSADSSKRATLKQFFADQDLYLYTANAFVYGVFKKQVIKEDVY
                     EPDWQTPERREYTKQVANLLAEFAPEGVNPSIQSAPLGFKPKVTGDHVVNEYTTNVID
                     VVAHLVELKKKTGRTVTLGLEPEPRCYLETTDETIQYFKNHLFSGPTAKRLAKLTGLN
                     EADAAQAMRDYTGVVFDIGHQSVGYEDIPGSLGKLVSAGVQIVKLQEAASMYMPAVTQ
                     KTVDALQTFAKTIYLSQTCQKKDGKTTWFLNLEDAFEAW"
BASE COUNT          385 a          656 c          655 g          319 t
ORIGIN      
        1 agccacaacg tagagcgcag gcttcagcct gcgcggagag acgagagacc gttgattaac
       61 gccgccattg tgggcatggg ttggtggggc cggaccatcg tggaatcggt ccaggccgac
      121 agcaacatcg tgcgcttcgt cgcgggcgcg acgcgaacgg cgtcgcccga ggtgaaggcc
      181 ttcgtcgacg cccagaaact gcgtctcgtg gactcctacg acgcgctgct cgccgaccgt
      241 tccgtggatg cggtggtgct ggccaccccc cattcgatgc actcgtccca ggtcatcgcg
      301 gccgccgccg ccggcaagca cgtcttctgc gagaagccgt tcgcgctgaa gaaggccgac
      361 gccgaagcgg ccgtggcggc gacggcgaaa gcgggcgtca cgctcggcct cggctacaac
      421 aggcgcttcc atcccgagat gacggcgctg cgcgagcgca tccgcacggg cgccctcggg
      481 accgtgcttc acgtagaagc gacgatgacg tttcccaacg cgctgcagct gaagccggag
      541 gcatggcgtg cacagcgcga cgagacaccc tgcggcgggc tcacgccgat gggtgtgcac
      601 gccatcgacg gcatgatcga cctgtgcggc gagatcgcgc aggtctactg tcagagcttt
      661 caccgcgtcg tgccggtgga cagcgacgac acgacctcga tgctgttccg catgaagagc
      721 ggcgcgtcag ggtatctcgg cacgatcacc gccaccgggc ccggcttcag cttccaggtg
      781 ttcggctcga acggctgggt gcgcctcgag ggcatgaccc acgtggcggg tgcctcctcg
      841 gaggaacgcc ggacgcgtct gttcagcacg tgcaagtttc aaccgatcaa gggaccggcc
      901 gaagtctggg aggccgcgcg cctcgacgtc acgcgcgcgt gcctggaggc gtttgccaac
      961 gccgcctccg gcggcgcgcc ctatctgatt ccgaccagcg agatgattca cggtgccgcc
     1021 gtgaccgagg ccgtggtgaa gtcggccgga tcagggaagg tagagcaggt atgaacctcg
     1081 ggaagggcct cggtcacctg acgtactcca cactcgtcca cccgaccgac aattgggatc
     1141 agctctggaa gagcgtcaac acctatctgc ccgcggtgaa ggcgcgcatg gcgccgcacg
     1201 agaagtttgg cgtgtgcctg cggatatcgg ccccgtcggc ggccgcgctc agcgcggatt
     1261 cgagcaaacg cgccacgctc aagcagttct tcgccgacca ggacctctac ctctacacgg
     1321 cgaatgcctt tgtctacggc gtcttcaaga agcaggtgat caaggaggac gtctacgagc
     1381 cagactggca gacgcccgag cgccgtgagt acacgaagca ggtcgcgaat cttctcgccg
     1441 agtttgcgcc ggagggtgtg aacccctcga tccagagcgc ccccctgggc ttcaaaccca
     1501 aggtcaccgg cgatcacgtc gtcaacgagt acacgacgaa cgtcatcgac gtcgtcgcgc
     1561 atctcgtcga gctcaagaag aagacgggca ggaccgtgac gctcggcctc gagcccgagc
     1621 cgcgctgcta cctcgagacg accgacgaaa cgatccagta cttcaagaac cacctctttt
     1681 cgggccccac ggccaagcgt ctggcgaagc tcactggcct gaacgaagcc gatgcggctc
     1741 aggccatgcg cgactatacc ggcgtcgtgt tcgacatcgg ccaccagtcg gtgggctacg
     1801 aggacatccc ggggtcactg ggtaaactgg tcagcgcggg tgtccagatc gtcaagctgc
     1861 aggaagcggc atccatgtac atgccggcgg tgacgcagaa gaccgtggat gcgctgcaga
     1921 ccttcgcgaa gacgatctac ctctcccaga cctgccagaa gaaggacggc aagacgacct
     1981 ggttcctgaa tctcgaagat gccttcgagg cctgg
//
LOCUS       DBNGSH010000771         3298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2293622, whole genome shotgun sequence.
ACCESSION   DBNGSH010000771 DBNGSH010000000
VERSION     DBNGSH010000771.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2293622"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="AC16CY_17710"
     CDS             <1..1821
                     /locus_tag="AC16CY_17710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3355692.1"
                     /translation="AARDLDAPSDDDDVAVFAGATRIRVLLARGELFQAGQRARDVLS
                     RAEASRLPLARVMSLSAHARVLMEAGDLVLAQQRVSELRAAARAAHVPLRFVRVRLAW
                     VDALRRAGRTREAERELRDLQRLRGVTPPLLRAGIDSRVRGDVRLPPREPASAAAHGV
                     AASLVAMGRDEEEDRDAVRRVVTFASESLRTSRLEVWSSDAGPDSTVLSVGTGLATTL
                     GSRVLDAGIVIDGRGGTPGGELGVPVRLGPRLVAALVARWPVDRAAPAHARELLELAA
                     AVASPRIEAMRLAAREAVHASTAIPELIGPSAAMGEVRRAVARAAAAPFSVLIEGESG
                     SGKELVARALHHLSPRRERRFCDVNCAALPDDLLESELFGHVRGAFTGAVADRPGLVE
                     DADGGTLFLDEVADLSPRAQAKLLRVVQQQEVRRVGATFSRKVDVRVISAANRDMRVE
                     AAEGRFRQDLLYRLDVVRIRVPPLRERPEDIPVLALHFWQAAAGRVGSRALLNHAVLT
                     ALARYHWPGNVRELQNVIAGLAVAAPARGQVKPTLLPPVLSSAAPVPAARLTEAREQF
                     ERRFIEVALARAGGRRARAARDLGLSRQGLLKMMSRLGLG"
     gene            1891..2943
                     /locus_tag="AC16CY_17715"
     CDS             1891..2943
                     /locus_tag="AC16CY_17715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3355693.1"
                     /translation="MAVRVGTFNCENLFSRSRIIDLSDSPAEQKRASAAIKAADALRK
                     VLKKKTYSQTDKKKIRDLLDVGRGFFTLEKDRGKLMVGNKVVASGAADFLGHIKFSRG
                     DISGTAIRNTGRIIKELNADILCIVEVEDRQLLGHFNSQVLTSNRFTNHMVIDGNDDR
                     GIDVGILSRKPLKSIRTHVNDRDDEGQIFSRDCAEYEMRLDDGRSLWVLCNHFKSKGF
                     GTPLFNDSRRERQAARVAKILEEQFDLTKDLVVVAGDLNDTPASAPLSPLLSLSGLHN
                     VVDTLPADGRFTHVFQGDRNQIDYLLVSTPLRDALTSVTIERRGMFSFPDRFSSITRL
                     SEEASDHAGVVAEFAI"
     gene            2979..>3298
                     /locus_tag="AC16CY_17720"
     CDS             2979..>3298
                     /locus_tag="AC16CY_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355694.1"
                     /translation="MPLQPGSILGPYEVTGPLGAGGMGEVYRARDGRLKRDVALKILP
                     AAFASDPERLARFQREAELLASLNHPGIAGIYGLEERDGLRALVLELVEGETLAERIA
                     RGPIP"
BASE COUNT          513 a         1053 c         1181 g          551 t
ORIGIN      
        1 gccgcgcgag acctggacgc gccgtccgac gacgacgacg tcgcggtgtt cgcgggtgcc
       61 acgcggatcc gagtgctgct ggcccggggc gagctgtttc aggcggggca gcgtgctcgg
      121 gacgtcctga gccgcgccga ggcgtcgcgg ctgccgctgg cgcgggtcat gtcgctgtcg
      181 gcgcacgcgc gcgtgctcat ggaggccggc gatcttgtcc tggcgcagca gcgcgtgagc
      241 gagcttcgag cggcggcacg cgcagcgcac gtgccgcttc gtttcgtgcg tgtcaggctg
      301 gcgtgggttg acgcgctccg acgggccggc cgaacgcgcg aggccgagcg cgagctcagg
      361 gacctgcagc ggctgcgcgg cgtcacgccg ccgttgctgc gcgccgggat cgacagcagg
      421 gtgcggggcg acgtgcgatt gccgccacgt gagcccgcgt cggccgccgc gcacggcgtg
      481 gccgcgtcgc tggtggcaat gggccgtgac gaagaagagg atcgtgacgc cgtccggcgt
      541 gtcgtgacct tcgcctcgga gtcgctgcgg acgagccgcc tcgaggtctg gtcgtcagat
      601 gccggcccgg acagcaccgt gttgagcgtc ggcacaggtc tcgcgacgac gctcgggtcc
      661 cgggtgctcg atgccggtat cgtcatagat gggcgaggcg gaacaccagg tggagagctt
      721 ggcgttccag tacggctggg tccgcgtctg gtggccgctc tggtcgcacg ctggccggtt
      781 gaccgggcgg cgccggcgca cgcgcgtgag ctcctcgagc tcgccgcggc cgtcgcgtcg
      841 ccccggatcg aagccatgcg gctggcggct cgggaagccg ttcacgcgtc aacggccatc
      901 cccgagctga tcggtccgag cgcggcaatg ggggaggtgc gtcgcgccgt agcgcgcgcg
      961 gccgccgcgc ctttttcggt cttgatcgaa ggggagagcg ggtcgggcaa agagctggtg
     1021 gcgagggcac ttcatcatct gagcccgcgc cgcgagcggc gattctgcga cgtgaactgc
     1081 gccgcgctgc ccgacgatct cctggaatcg gagctgttcg ggcacgtgcg cggcgccttc
     1141 accggggccg tggccgatcg gcccgggctc gtcgaggacg ccgacggtgg cacgttgttc
     1201 ctggacgagg tcgccgatct gtcgccgcgc gctcaggcga agctgctgcg cgtcgtgcag
     1261 cagcaggagg ttcgtcgcgt cggcgcaacg ttcagccgca aggtggacgt gcgcgtgatc
     1321 agcgccgcca accgcgacat gcgcgtcgag gcggcggagg ggcgtttccg ccaggacctg
     1381 ctgtatcggc tcgacgtggt gcgtattcgc gtgccgccgt tgcgtgaacg gcccgaggac
     1441 attcccgttc tcgcgctgca cttctggcag gccgccgcgg gtcgggtcgg gtcgcgcgcc
     1501 ctgctgaatc acgcggtcct gacggcattg gcgcgatatc actggccggg caacgtgcgt
     1561 gagctgcaga acgtcatcgc ggggctcgcc gtcgccgcgc ctgcgcgtgg ccaggtgaag
     1621 ccgacgctgc tgccgccggt cctgagcagc gcggcgccgg ttccggccgc gcggctgacg
     1681 gaggcccgcg aacagttcga acggcgcttc atcgaggtcg ccctggcgcg ggcgggcggc
     1741 cgcagagcca gagccgcccg ggatctcggg ttgtcgcggc aagggctgct gaaaatgatg
     1801 tcgcggctcg gacttggctg acggtagtac tatccagctg ttcggtttcc gcctggtcgt
     1861 cgtttcagaa gtaagtcagg agctggatcc atggccgttc gcgtcggcac gttcaattgc
     1921 gaaaacctct tcagccgctc gcgaatcatc gatctcagcg actcgcctgc agagcagaag
     1981 cgggcatcgg cggcaatcaa ggcggctgac gcgctcagga aggtcttgaa gaagaagacg
     2041 tacagccaga ccgacaagaa gaagatccgc gacctgctcg acgtgggcag gggattcttc
     2101 acgctcgaga aagaccgtgg aaagctcatg gtcgggaata aggtcgtcgc ctccggcgcc
     2161 gccgatttcc tcggtcacat caagttcagc agaggggaca tcagcggcac ggccatcagg
     2221 aacaccggcc ggatcatcaa ggagctgaac gcggacatcc tgtgcatcgt cgaagtcgag
     2281 gaccgccagc tcctggggca cttcaattcg caggttttga cgagcaatcg cttcacgaac
     2341 cacatggtca tcgacgggaa cgacgaccgc ggcatcgacg tcggaatcct gagcaggaag
     2401 ccgctgaaga gcatccgcac gcacgtgaac gacagggacg atgaaggtca gatcttctcg
     2461 agagactgcg cggagtacga aatgaggctc gacgacggcc gatccctgtg ggtgctgtgc
     2521 aatcacttca agagcaaggg gttcggcacg ccgttgttca acgactcgcg ccgcgagagg
     2581 caggcggccc gggtggcgaa gatcctggag gaacagttcg atctgacgaa ggatcttgtc
     2641 gtcgtcgccg gcgatctcaa cgacacgccg gcatcggcgc cgttgtcgcc gctgctctcc
     2701 ttgtccggcc ttcacaacgt ggttgacact cttcccgccg atggccgctt tacccacgtc
     2761 ttccagggcg acaggaatca gatcgattac ctgctggtct cgacgccctt gagagacgcg
     2821 ctgacgagcg tcacgatcga gcgacgaggc atgttcagct tccccgatcg cttttcgtcc
     2881 atcacccggc tgtccgaaga agcatccgat cacgcaggcg tcgtggcaga gtttgcgatc
     2941 tgagcgccgc tgtccgctga gttacaatcc gcccggccat gccgctccag ccaggctcga
     3001 ttctcgggcc ctacgaagtg acgggtccgc tcggcgccgg cggcatgggc gaggtctatc
     3061 gcgcccgcga cggacggctg aagcgcgacg tggcgctcaa gatcctgccg gcggcctttg
     3121 cctcggatcc cgagcgcctc gcccggtttc agcgtgaagc ggagctgctc gcgtcgctga
     3181 atcaccccgg catcgccgga atctacggac tcgaggaacg cgacggtctg cgtgccctcg
     3241 tcctcgagct ggttgagggg gagacgcttg ccgagcgtat tgcgcgcggg ccgattcc
//
LOCUS       DBNGSH010000772         3090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3820732, whole genome shotgun sequence.
ACCESSION   DBNGSH010000772 DBNGSH010000000
VERSION     DBNGSH010000772.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3820732"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..2785
                     /locus_tag="AC16CY_17725"
     CDS             122..2785
                     /locus_tag="AC16CY_17725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3355695.1"
                     /translation="MKRSLRSWLWRVPVREEVDEEIAFHVEMRTRELVDKGLDAKAAR
                     EVVLARIGDTGRLRRTCVDLGRKRDREMRLTQWLADLRDDVAVAVRQLKASPAFTTVA
                     VLTLALGIGANSAMFAVADATLLRPLPYPGGDRVVAVSELRRDGTRGPANPLDFVDWR
                     ERNRAFEAMAGVMSGETSITGADGFVQSVLTQAVTTDFFDVLGILPIAGRTFQPGDET
                     PMSNVVVISEGLWQSHLGGDPAAVGGPIRLGGRTLTVAGVMPASFQFDIPGLPGLGPS
                     QVWTILNPPRSRAPEQRYPHYLPVIARLKPGVTIDAARQEMAAISDAIAEESPATNKG
                     HRATVDSLRDRLASRELRLTSLLLFGVVMFVLLMCCANVANLLLARTSERSRELAVRS
                     ALGAGRQRIVRQLLTESLVLAALGGMLGMAIGMAILKAAPSLVPPGLLPVSVPLAFDQ
                     RVLLFSVATAFAVAILYGLAPAWQATGTSLANVMSLNSRTAAGGSSRLRRGLAMAEVA
                     VAVLLLCGAGLLLRTLLTLQDVDAGNRAGELLTTQVRPGPGRTPDALRQLYANIGSAV
                     RHAPGVRDVAWGSALPFAGQFFGQAIQVDGDPLLPPANRPGATYQIVGPDYFRLVGVP
                     VLEGRVFTDADATNAPQVAVVDEEFVRRYLKGRTPIGSRVSVNAMTQSQAVLREIVGV
                     VKHIKERPAEPEAQPQLYVPLAQDTWWVVSLVVQPAGGRADAIAPSVRRAVASVEPNL
                     SVVFRTLTTIHTQAVSRQRFRAVLVGAFALLALTLALVGVFGVLAYSVQQRTREFGVR
                     IALGASAVSVLRLVMSSAVVVIGVGVGIGLVAAAVLSRSISTFLFGVRPLDPLTYLAV
                     ALVLIVTAAIAAAAPAWRAARVDPVVAFRNE"
     gene            2825..>3090
                     /locus_tag="AC16CY_17730"
     CDS             2825..>3090
                     /locus_tag="AC16CY_17730"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR3355696.1"
                     /translation="MKRSLRSWLWRVPLDQEVDDEIAFHIEMRTRELVAKGLDGRTAR
                     EVVLARIGDTGRLRRTCIDLGRKRDRHMRVTQWLGELRDDVKFA"
BASE COUNT          454 a         1007 c         1079 g          550 t
ORIGIN      
        1 ccgagctcgg ccgccgggcc aagctctatc gcctcacacg gcaggggcgg gcacaactgg
       61 cggccgaaac cgacgcctgg cggcaattcg ccgccggggt gtccaagatt ctgctggcgc
      121 aatgaaacgc tcccttcgct cctggttgtg gcgcgtcccc gttcgcgaag aggtggacga
      181 agagatcgcc ttccacgtcg agatgcggac gcgcgagctg gttgacaagg ggctggacgc
      241 gaaggcggcg cgcgaggtgg tgctcgcgcg gattggcgac accggccggc tcaggcggac
      301 ctgcgtcgac cttgggcgaa agcgagatcg tgagatgcgg ctgacgcaat ggctggcaga
      361 cctcagggac gatgtggccg ttgcggtgcg gcagctgaag gcatcgcccg cgttcacgac
      421 agtggctgtc ctgacgctcg cgctcggtat cggtgccaac agcgcgatgt tcgccgtcgc
      481 cgatgcgacg ctgctgcgtc cgctgcccta tccgggcggg gatcgggtgg tggccgtgtc
      541 cgagctcaga cgcgacggga cgcgcggccc ggccaatccg ctcgacttcg tcgactggcg
      601 cgagcgcaat cgcgcgttcg aggcgatggc tggcgtgatg agcggtgaga catcgattac
      661 cggcgcggat ggcttcgttc aatcggtgct gacgcaagcc gtgacgaccg acttcttcga
      721 cgtgctcgga atcctgccga tcgccggccg cacgtttcaa ccgggcgatg aaacaccgat
      781 gtcgaatgtc gtcgtgatca gcgaaggact ctggcaaagc cacctgggag gcgatccggc
      841 ggcggtcgga ggaccaatcc gcctcggagg ccgaacgctc acggtcgccg gcgtgatgcc
      901 ggcgtcgttt cagttcgaca ttcctggact cccgggtctt gggcccagcc aggtgtggac
      961 cattctgaat cctcctcgca gtcgcgcccc cgagcagcga tatccacact acttgccggt
     1021 gatcgcccgc ttgaagcccg gtgtcaccat cgacgctgcg cgccaggaaa tggccgcaat
     1081 ctcggacgcc atcgccgagg agtcgcccgc cacgaacaag ggccatcgcg cgacggtgga
     1141 ttcgctgcgc gatcggctgg ccagccgcga actgcgcctg acctcgctgc tgctgttcgg
     1201 cgtcgtgatg ttcgtgctgc tgatgtgctg cgcgaatgtc gccaatctgc tgctggcgcg
     1261 cacgagcgag cgctcacggg agcttgcggt ccgatcggcg ctcggcgcgg gacgccagcg
     1321 catcgtgcgc cagctgctga ccgagagcct cgtactcgcg gcactgggcg gcatgctggg
     1381 catggcgatc ggcatggcca ttctgaaggc ggcgccatcg ctcgtcccgc caggtctgct
     1441 gccggtgtcg gttccgctag ccttcgatca acgtgtgctg ctcttctcgg ttgccaccgc
     1501 attcgccgtc gcgatcctgt acgggctggc gccggcctgg caggcgacgg gcacgtcact
     1561 ggcgaacgtc atgtcactga actcgcgcac cgcggctggg ggaagctcga ggcttcggcg
     1621 cggacttgcg atggccgaag tcgccgtcgc agtgctgctc ctgtgcggcg ccggcttgct
     1681 cctgcgcacg ctgctcacgc tccaggacgt cgacgccgga aaccgggccg gcgagctgct
     1741 gaccacccag gtcagacccg gacccggcag aactcccgac gcactgcgtc agctctacgc
     1801 aaacatcggg agcgccgttc gccatgcacc cggcgtgcgc gacgttgcct ggggcagcgc
     1861 gttgccgttc gccgggcagt tcttcggaca ggccattcag gtcgacggcg acccgctgct
     1921 gccccccgcg aatcgtcccg gcgccaccta tcagatcgtc ggccccgatt acttccgtct
     1981 ggtcggcgtc cccgtgctcg aagggcgggt gttcaccgat gccgacgcca ccaacgcccc
     2041 gcaagtcgcc gttgtcgatg aggagttcgt gcgccgctat ctgaagggcc ggacgccgat
     2101 cggttcacgc gtgtcggtca acgcgatgac gcagtcacag gccgtgctgc gcgagatcgt
     2161 cggcgtggtg aagcacatca aggagcgacc ggcagaaccc gaggcacagc cacagctgta
     2221 tgtgccgctc gcgcaggaca cctggtgggt ggtctcgctg gtcgtgcagc ccgcgggtgg
     2281 acgggctgac gccatcgcac cgtcagtgcg ccgcgcggtc gcgagcgttg agcccaatct
     2341 gtcagtggtg ttccggacgc tcacgacgat tcacacgcag gcagtatcgc ggcagcgctt
     2401 ccgcgctgtc ctcgtcggcg cgttcgccct gctcgcgttg acgctggcgc tcgtcggcgt
     2461 ctttggcgtg ctcgcgtact cagtgcagca gcgtacacgg gagttcggcg tgcgcatcgc
     2521 gctcggcgca agcgcggtca gtgtgctccg gcttgtcatg tcgagtgccg tcgtcgtcat
     2581 cggggtgggt gtcggcatcg ggctcgtcgc cgcggccgtc ctgagccgtt cgatctcgac
     2641 gttcctgttt ggcgtgcggc cactagatcc gctgacgtac ctggccgtgg cactggtcct
     2701 gatcgtgacg gcagcgatcg ccgcggctgc cccggcctgg cgggccgcgc gcgtcgatcc
     2761 ggtggtggcg tttcgcaatg agtagtgggg gcgtagtgtc cggctttagc cggaccgtgg
     2821 accgatgaaa cgctcgcttc gttcgtggct ctggcgcgtg ccgctcgatc aggaagttga
     2881 cgacgagatc gcgtttcaca tcgagatgcg cacgcgcgaa ctcgtggcga aggggctgga
     2941 cggcaggacg gcgcgcgagg tggtgctcgc gcgaatcggg gataccggtc gactcaggcg
     3001 cacctgcatc gaccttggac gaaagcgaga tcgtcacatg cgcgtcacgc agtggctcgg
     3061 cgagttgcga gatgacgtga agttcgcctt
//
LOCUS       DBNGSH010000773        10368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3820779, whole genome shotgun sequence.
ACCESSION   DBNGSH010000773 DBNGSH010000000
VERSION     DBNGSH010000773.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10368
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3820779"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /gene="groL"
                     /locus_tag="AC16CY_17735"
     CDS             complement(<1..1247)
                     /gene="groL"
                     /locus_tag="AC16CY_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786183.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3355697.1"
                     /translation="MAKQIVYGEQSRQAILRGINQLANAVRVTLGPKGRNVILDRKFG
                     SPTITKDGVTVAKEIDLGDSLENMGAQMVREVASKTSDTAGDGTTTATVLAQAIYREG
                     AKNVVAGANPMELKRGIDKAVAVVVAEIKKMARPVSGNMVAQVGMISANGDQAIGTII
                     AEAMQKVGKDGVITVEEAKTLETSLDIVEGMQFDRGYLSAYFVTDPERMEVVLENPVI
                     LIHEKKISSMKDLLPVLEQVAQLGRPLLIIAEDIEGEALATLVVNKLRGTLKAAAVKA
                     PGFGDRRKATLDDIAILTGGRAITEDLGIKLENIKIEDLGQAKKVTIDKDTTTIIEGA
                     GTSSAIEGRVKQIRTQVEDTTSDYDREKLQERLAKLVGGVAIIKVGAATETEMKEKKA
                     RVEDAMHATKAAVEEGIVAGGGVA"
     gene            complement(1259..1552)
                     /gene="groES"
                     /locus_tag="AC16CY_17740"
     CDS             complement(1259..1552)
                     /gene="groES"
                     /locus_tag="AC16CY_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3355698.1"
                     /translation="MKVTPLHDRLIIQRLEEGEQRVGGIIVPDSAREKPQRGKVFAVG
                     QGKVRDDGSRQHPDVKDGDTILFGKYSGQEIKIDGEEYLILREDEVLAIIDNK"
     gene            1752..1883
                     /locus_tag="AC16CY_17745"
     CDS             1752..1883
                     /locus_tag="AC16CY_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355699.1"
                     /translation="MGDRGGKKDKEKNKQQHVQKQKQEEQRKQDKARPGTPLRHHGV"
     gene            complement(2311..3309)
                     /locus_tag="AC16CY_17750"
     CDS             complement(2311..3309)
                     /locus_tag="AC16CY_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355700.1"
                     /translation="MSLTNTSRLSRVFLVAALSAAPVANAQSVDVDLGGMVLSVSSAS
                     SAQVFHIVDQLSEWDQYAHKAYGRWAAKTLNFNDQDRRLLQQHAELRRARGWGKGFEQ
                     AFLVTDSIDAAATNAIAAGLLTSAEATTERNILNHFSPKVAALIEQQRDRIDAFRNQL
                     VSDRATLTPLVQKLARFAEATSPIRVSVFLVANPEENSGGGGANGGRLVVEVPGPDSM
                     GFLLHEALHVFLAPHAEAIRAAAASAGLSFQALNEGIAYALAPGLTDDNAQVDHLAET
                     LARHLIRGTPASDAYVQSSMIASVVRPLLRASLDRGETLSGFLPKAASRWRTISPR"
     gene            3614..4627
                     /locus_tag="AC16CY_17755"
     CDS             3614..4627
                     /locus_tag="AC16CY_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355701.1"
                     /translation="MARNRSLIRLFTLLTTVTLALLDASAQGRGSTPARTPDGKPDLA
                     GTYSFSTVTPLQRPDALAGKTTLTDEEAATFEKAENTRLNRDLFDPEKGQPSAGYAPR
                     SQGGVLSYNEFWYERGNTLTRDKRTSLIVDPPDGRIPFTDAARRRVAEMRQRSDSGLG
                     DSYADRPLADRCLQGFNSGPPMTPGSYNNNVEIVQAPGVVVIVNEMVHNARVIPTDGR
                     PHTTLRQWSGDSRGRWDGDTLVVETINFRRETSLQGSTANTTLVERFTRVDAETLKYE
                     FTVTDPTSYTRPWSAMVPLIRIREPLFEYACHEGNYSLRNILAGAREQERAREREKGE
                     PIR"
     gene            complement(4657..5826)
                     /locus_tag="AC16CY_17760"
     CDS             complement(4657..5826)
                     /locus_tag="AC16CY_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355702.1"
                     /translation="MTRQAWLTVVLYVSAAVFGWQQVARSHGSITTTVLFDREIVRIL
                     DRHCVMCHSEQSLSFPLSTYEQTWVRGRDIRTEILRRHMPPWSAVAGYGEFLNDNNLT
                     VRETQFMVSWVEGLGPRNAGRVFLNVARPDSTTSEIRATAHVGHWQVGTPDLSRELAP
                     TRIDAGKADFVHRTIVDPGLTSERRMRALEYQPGDRRVVRAATFFVEGTGQWLGSWTP
                     WYGFVTLPDGVAARLPARTRIVAEIHYRGASEPVVERGTLGLFFDRAATSAIPSDIVL
                     DPRTVTSAGGPTQKLRASTRMTRDTHVWALRPDLVPGLTSIEVSARAPDGGTEILLLA
                     RNPAIEWPTPFVLKTPRLLKRGTEVSVVAESREGRVQAARGDAQARPLRLTISRY"
     gene            complement(5823..6803)
                     /locus_tag="AC16CY_17765"
     CDS             complement(5823..6803)
                     /locus_tag="AC16CY_17765"
                     /inference="COORDINATES: protein motif:HMM:NF039593.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIR3355703.1"
                     /translation="MPRVLTASAAVLLLLSGLFVSARQAAQDALDTTAFVCPMHPDYT
                     LDVAGTCPRCGMALVKATPFDVRDYALEFRTTPALVRAGQQATWRFGIRHPGTGEPVR
                     SLEVVHERPYHLFVISQDMEHFQHIHPQQQADSSWAIDVTLPKAGYYSVISDFLPSGG
                     ASQLISRPLVTAGYTGDLAGDSAALVPDASAVKVAGAITARVSLDPPTFVAGLYGHLI
                     FQLTDTNTGRPITDLQTYLGAFGHTLIMSEDMLDYVHSHSLDILATGDDDAVPQFLIP
                     PGADLETLRGGPDVTFDGLMPKPGRYRAWTQFRRDDVVHTFTTTFSVVAP"
     gene            complement(6781..8700)
                     /locus_tag="AC16CY_17770"
     CDS             complement(6781..8700)
                     /locus_tag="AC16CY_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3355704.1"
                     /translation="MGRLVRLGVLCLSAVAIRPLDAFDAQCVDHSKDPAGCQPSTFDT
                     PLAQMPSVRVNRLGQIDPTSSEADAKAGAFELEKRLNLFRNFEHLHWVVTVPSVRDAA
                     TGAWRGGDLDGAGDGRGLGVAGQCLFVGHANGAGVRRAINIFRIQPNPDMQPPLQVGE
                     IPAMTEGNQGFDDRELRALIFKTSSGEDRYIMVRNAGTNTLGRMQSYQIDMNTCLPTF
                     TSEVTDFHSQSHEFYLWHDPANSNRVLVYMTNWTGGLPDPERPGLTVPDLIVLAVTDE
                     RTGALLPKARMLAGFTLQDVGGPPIDERPDATGLFSDGRFLDFSDSKNRLGQAGNFQN
                     REQNRLHSVSVTDDGERVYVAGTTAGFYVLDSEAIARATDAQLAAGTAGCHQRSTIVA
                     TAGGAIDAAKLPALANDCLHMVVNNDPGLKAFLISTASPETKASRYLVLLTRSRFDVY
                     PPVNALPTGTHSAVFVPNRPARVRGNAKGRPAFVWLSDENGGCPLNHARIVSVEAEAT
                     PIMIGAFAIPDNQVEECLDQATTEPNGAPRRRVPQQNHNPTIFKNLVFTTWYGHGLRA
                     IDISNPYNPREVGHALTIPQGVARTYPVFKDGLIYWVDNDTGLHVARYTGPRRDELPG
                     PGSGTYEGNATSPHR"
     gene            8809..9261
                     /locus_tag="AC16CY_17775"
     CDS             8809..9261
                     /locus_tag="AC16CY_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3355705.1"
                     /translation="MQRCTRLGFAAILVSLAAVSSHVSAQRQVTMVTRLYTGTDGQSH
                     AEDIEVAWRPAKLRAELSDSESVRVTSANFLRWPRGFVWAGHPASRRQYVVIVSGRGE
                     VDVGGGRKVQLAPGRVLLAEDTTGKGHTTRVGPDEDLVMLLVPLAQEP"
     gene            9295..9813
                     /locus_tag="AC16CY_17780"
     CDS             9295..9813
                     /locus_tag="AC16CY_17780"
                     /inference="COORDINATES: protein motif:HMM:NF015862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR3355706.1"
                     /translation="MRSRDRVLPIVVTISAFFASHAFGAQAARDIKTTRDVKVISSAG
                     ARALADACTVWAEQNKQVVAMAVLDWGGNLIEFHAMEGATMNAIDTALLKAKSALRWR
                     RPTSETNKMVRSGENLAPTFMNDFPQPGALPIVLGGQVIGAMGVSGADGEKCAQAAID
                     AVFKGQATTTAR"
     gene            9853..10368
                     /locus_tag="AC16CY_17785"
     CDS             9853..10368
                     /locus_tag="AC16CY_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355707.1"
                     /translation="MTRTSALVVVLISVAASASAQTAKPAAESDAIAVLTREIRALRE
                     EITETARAGLRLQLLTARIQAQEQRIIYLDRQRAEAVTRRASAEQARDAIATQIQTFA
                     GGDTSKLTAEQRREMEPMIAVFKKQVATHDSLVAQLETEEIDAANALAQEQGRWADLN
                     QRLEDLERSLK"
BASE COUNT         1840 a         3310 c         3417 g         1801 t
ORIGIN      
        1 gcgacgccgc cgcccgcgac gatgccttct tccacggctg cctttgtcgc gtgcatcgca
       61 tcttcgacgc gcgccttctt ctccttcatc tcggtttcgg tggccgcacc gaccttgatg
      121 atcgcgacgc cgcccacgag tttcgccagc cgttcctgca gcttctcccg gtcatagtca
      181 gaggtcgtgt cctcgacctg ggtgcgaatc tgcttcacgc gcccctcgat cgcggacgac
      241 gtgccggcgc cctcgatgat cgtcgtggtg tccttgtcga tcgtgacctt cttcgcctgg
      301 cccagatcct cgatcttgat gttctcgagc ttgatgccga ggtcctcggt gatggcgcga
      361 ccgccggtga ggatggcgat gtcatccagc gtcgccttgc ggcggtcacc gaagccgggc
      421 gccttcactg cggctgcttt cagcgtgccc cgcagcttgt tgacgacgag cgtcgccagc
      481 gcttcaccct cgatgtcctc ggcgatgatg aggagcggcc ggccgagctg cgcgacctgc
      541 tccaggacgg ggaggaggtc cttcatcgat gagatctttt tctcgtggat gaggatcacg
      601 ggattctcga gtacgacctc catgcgttcg ggatcggtca cgaagtacgc cgagagatac
      661 ccgcgatcga actgcatgcc ctcgacgatg tcgagtgacg tttccagcgt cttggcttct
      721 tcgacggtaa tcacgccgtc cttcccgacc ttctgcatgg cttcggcgat gatggtgccg
      781 atcgcctggt cgccattggc ggagatcatc cccacctgcg cgaccatgtt tcccgacacg
      841 ggcctggcca tcttcttgat ctctgcgacg acgactgcga cggccttgtc gatgccgcgc
      901 ttcagctcca tcgggttggc gcccgcaacc acgttctttg cgccttcgcg gtagatcgcc
      961 tgtgccagca cggtggccgt cgtcgtgccg tctccggccg tgtccgacgt cttgcttgcc
     1021 acctcacgga ccatctgcgc gcccatgttc tcgagcgaat caccgaggtc gatctctttt
     1081 gctaccgtca ccccatcctt ggtgatggtc ggggagccga acttccggtc gaggatgacg
     1141 ttgcgtccct tcggacccag ggtgactctg accgcgttcg ccagttggtt gatcccgcgg
     1201 aggatagcct ggcgggattg ctcgccgtag acgatctgtt ttgccatgta tgcctgccct
     1261 acttgttgtc gatgatcgcg agcacttcgt cctcacgcag gatgaggtac tcctcgccgt
     1321 caatcttgat ctcctggccc gagtacttcc cgaacaggat cgtgtcgccg tctttcacgt
     1381 cagggtgctg gcggctgcca tcgtcccgga ccttgccctg cccaacggcg aagaccttgc
     1441 cacgctgcgg cttctccctg gcggaatcgg gcacgatgat gccgccaacg cgctgttcgc
     1501 cttcctcgag acgttgaatg atgagacggt cgtgaagcgg tgtgactttc atgtgactcc
     1561 aggtctggtc gtgaaccggg gggcgatcgc cagcagggcg gtctggacag gagtgaacga
     1621 cgagacggta ccgagcatac tcggcccgcg gcgcgcggac tgtgcagaac aggacatctg
     1681 ctgaggcgcg ggccagcaga gactcaagat cacgcgtcgt cagaggtcaa cgttggctga
     1741 aaggagcggg catgggagac aggggcggca agaaggacaa ggaaaagaac aagcaacagc
     1801 acgtccagaa acagaaacaa gaggaacaac ggaagcagga caaggcccgg ccagggactc
     1861 cgctacgcca ccacggcgtc tgacgtgtac cggcgaggtt cgttcttcga atcagtggtc
     1921 gatcgcacga acacgccatc gctggcgcgg gacagaaagt tctccctcac gagcgccctg
     1981 aacatcgcgg cgcataccga gggtgccacc ccaaacgcgc gcatggcttg cggcttggtc
     2041 aattcgaggc ccggcgttct tccgtatctc tcgcggactt gctgcaacag ttcagcaagt
     2101 ggtgcggagt cggcggggca tgtgatcaga gattgcatgt tggaagggta cgacgcgttg
     2161 cggaaggccg ctgtcccaaa gcgcacaggt cgagacacag aacagccccc tcggcaacaa
     2221 ccgcactcgt ctggtcacga gcggcaccga gcgcttcaag aagaccgttg ccacgttgca
     2281 accactcgaa ggttgcgcac gagcgcactt ctaacgcggg gagattgttc gccacctgct
     2341 cgcggctttc ggcaggaatc cactgagcgt ttctcctcga tcgagcgatg ctcgaagcag
     2401 cggacgaacc acgctcgcaa tcatgctgga ctgcacgtac gcgtcagatg ccggcgtccc
     2461 gcggatcagg tgccgcgcga gcgtttcagc gagatgatcg acctgtgcgt tgtcgtcggt
     2521 gagacctggc gcaagcgcgt aggcgatgcc ttcgttgagg gcctggaagc tcagtcccgc
     2581 ggacgcggct gcggcacgaa tcgcctcggc atgtggggca aggaagacgt gcagcgcttc
     2641 gtgcaacagg aaacccatcg agtctggccc cggcacttcc accacgaggc gtccgccgtt
     2701 ggctcctccg ccaccgctgt tctcttccgg attggcgacg agaaagacgg acaccctgat
     2761 cggactcgtc gcctctgcga aacgtgcgag tttctgaacc aacggtgtga gtgtggcgcg
     2821 atcggagacg agttggttcc tgaaggcatc gattcgatcg cgctgctgtt cgatcagcgc
     2881 agcgaccttc ggcgaaaagt ggttgaggat gttccgttca gtcgtggcct cggccgaagt
     2941 cagcaggccc gcggcgatgg cgttggtcgc cgcggcatca atggaatccg tgacgaggaa
     3001 ggcctgctcg aacccctttc cccagccgcg agcgcgccgc agttccgcgt gttgttgcaa
     3061 cagccgccgg tcttgatcat tgaagttgag tgtcttcgcc gcccagcgcc cgtacgcctt
     3121 gtgcgcgtac tgatcccatt ccgacagctg atcgacgatg tgaaagacct gggcgctcga
     3181 ggcactggat accgagagga ccatgcctcc cagatcgacg tcgacgctct gcgcattcgc
     3241 gacgggcgcg gcggacagag ccgcaacgag gaagactcgc gatagtctcg aggtgttcgt
     3301 gagactcatg gcggaccgtt cgcagccgtc agccgctgcg atcgagaaga tatcccatcg
     3361 cgccaacaat caccagcacg acgacgatgt tctgggatcc tcgacgcgcg ggccccgcga
     3421 ccgtcagcga tgcttcgccc tggaccgcgg cgcggcggtt ttcgacttgg ctttcgccgg
     3481 gcgcttaggc ttcgccggac gctacccccg tcgctggttg atctccgcgc ggatccgtgg
     3541 acgatttcct tgcaccgtcc tggcaagctt tgctaccgtg caaccgatcg gcgcccgccg
     3601 gcgtggagga gatatggcgc gcaatcgctc gctgatccgg cttttcacgt tgctgacaac
     3661 ggttacgctg gcgctgcttg atgcgtcggc ccagggccga ggatcgacgc cagcccggac
     3721 gcccgatgga aagccggacc tcgcaggcac ctattcgttc agcacggtca cgccgctcca
     3781 gcggccggat gcgctcgcgg gaaaaacaac gctcactgac gaagaggctg ccacgttcga
     3841 aaaggcggag aacacgcggc tcaaccggga cctgttcgat cccgaaaagg gccagccaag
     3901 cgccggctat gcgccgcgtt cccagggcgg cgtgctgtcg tacaacgagt tctggtacga
     3961 gcgcggcaac acgctgaccc gggacaagcg cacgtcgctc atcgtcgatc ctccggacgg
     4021 aaggatcccg ttcacggatg ccgcgcgacg cagggtcgcc gagatgcggc agcgatcgga
     4081 ctccggtctt ggcgactcgt atgcagacag gcctctcgcg gaccggtgcc tccaagggtt
     4141 caactcaggc ccaccgatga cgccaggctc ctacaacaac aatgtggaaa tcgtgcaggc
     4201 gccgggagtt gtcgtgatcg tcaacgaaat ggtgcacaac gccagggtca tccccacgga
     4261 cgggcgcccg cacaccaccc ttcgccagtg gtcgggagac tcccgtggcc ggtgggacgg
     4321 cgacacgctc gtcgtcgaaa cgatcaactt ccgccgcgag acgagcctgc agggctccac
     4381 cgcgaacacg acactggtcg aacgcttcac gcgagtcgac gccgagacgc tcaagtacga
     4441 attcactgtc accgatccga cgtcgtacac caggccgtgg agcgccatgg tgccgctgat
     4501 acgaatccgc gaaccgctgt tcgagtacgc ctgccacgag gggaactatt cgctgcgaaa
     4561 cattctggcc ggtgcgcgcg aacaggagag ggcgagggag cgggagaagg gcgagccgat
     4621 ccggtagtcg gccaccgccc cgatggccgg gatctctcaa tacctgctga tcgtcaggcg
     4681 cagaggtctg gcctgcgcat cgccacgggc cgcttgaacg cggccctcac ggctctcggc
     4741 gacgacggag acctcggtgc cgcgcttcaa caagcgcggt gtcttcagga caaacggtgt
     4801 cggccactcg atcgcgggat tccttgccag caatagaatc tcggtcccgc cgtcaggagc
     4861 gcgcgcggag acttcgatcg acgtcagccc gggcacgagg tccggccgaa gcgcccacac
     4921 gtgcgtgtcc cgcgtcattc gggtcgacgc gcgcagcttc tgcgttggcc cgccggcgga
     4981 ggtgacggtc ctcgggtcga gcacgatgtc cgacgggatg gctgaagtcg ccgcccgatc
     5041 gaagaagagc ccgagagtgc ctcgttcgac gaccggctcg ctggcccctc tgtagtggat
     5101 ctcggcgacg atgcgcgtac gggcgggcag ccgagcggcg acgccatcag gcagcgtgac
     5161 gaatccgtac cacggcgtcc aactgcccag ccattgcccc gtgccttcga cgaagaacgt
     5221 cgccgcccga accacgcgac gatccccggg ctgatattcg agtgcgcgca tgcggcgctc
     5281 cgacgtcagc cctggatcga cgatggttcg gtgaacgaag tcggccttgc cagcgtcgat
     5341 gcgtgtcggg gccagctcgc gggacaggtc cggcgttccg acctgccagt ggcccacgtg
     5401 cgcggtcgcg cggatctcgg atgtcgtcga atccgggcgc gcaacgttga gaaacacccg
     5461 gcccgcgttt cttggtccga gaccctccac ccaggacacc atgaactgcg tttcacgaac
     5521 cgtcaggttg ttgtcgttca ggaactcgcc gtagcccgcg acggccgacc acggcggcat
     5581 gtgacgacgg aggatctcgg ttcggatgtc gcggccacgc acccaggtct gctcgtaggt
     5641 cgagagcggg aaggacaatg actgttcgct gtggcacatc acacagtgac ggtcgaggat
     5701 gcgcacgatc tcccggtcga acagcaccgt cgtcgtgatc gatccatgtg agcgggcgac
     5761 ctgctgccag ccgaacacgg cggcggacac atacaacacg accgtcagcc aggcctgtcg
     5821 agtcacgggg caaccaccga gaaggtcgtc gtgaaggtgt gcacgacatc atcccgacgg
     5881 aactgcgtcc aggcgcggta gcgtccgggc ttcggcatca ggccgtcgaa ggtcacgtcg
     5941 ggaccgccgc gcagcgtctc gaggtcggcg cctggtggaa tgaggaactg cggcacggcg
     6001 tcatcgtcac ctgtggcgag aatgtccagc gagtgcgagt gcacgtagtc gagcatgtcc
     6061 tcgctcatga tgagcgtgtg accgaaggcg ccgagatatg tctgcaggtc ggtgatggga
     6121 cgtccggtgt tcgtgtcagt cagttggaag atgaggtgac cgtagagccc ggcgacgaac
     6181 gtcggcggat cgagcgatac gcgtgcggtg atggcgccgg caactttcac cgcgctcgcg
     6241 tccgggacga gagcggcgct gtcccccgcg agatcaccgg tatacccggc cgtgacgagc
     6301 ggacgcgaga tgagctgcga ggcgccaccg ctcggcagga agtccgagat caccgagtag
     6361 taacccgcct tcggcagcgt cacgtcgatc gcccacgacg agtccgcctg ctgctggggg
     6421 tgaatgtgct ggaagtgctc catgtcctgg ctgatcacga acaggtgata cggccgctcg
     6481 tgtacgacct cgagcgacct gaccggctcg ccggtgccgg ggtgtcggat gccgaagcgc
     6541 caggtcgcct gctgtccggc gcggacgaga gcgggcgtcg ttcgaaactc gagcgcgtag
     6601 tcgcggacgt cgaacggcgt agccttgaca agcgccatcc cgcaccgagg gcacgtgccg
     6661 gcgacgtcca gcgtgtagtc cgggtgcatc gggcagacga acgcggtggt gtcgagcgcg
     6721 tcttgtgcgg cctgacgggc gctcacgaag agacccgaca gcagaagaag taccgccgcg
     6781 ctagcggtga ggactcgtgg cattgccttc gtacgtaccg ctgcctgggc ccggcaactc
     6841 gtcgcgccgc gggccggtat agcgcgcgac gtgcagcccc gtatcgttat cgacccagta
     6901 gatcaggccg tccttgaaca ccgggtaggt ccgggccacg ccttgcggaa tggtcagcgc
     6961 gtgtccgacc tcgcgcggat tgtaggggtt cgagatgtcg atcgcgcgca ggccgtggcc
     7021 ataccacgtg gtgaagacga ggttcttgaa gatcgtcggg ttgtgattct gctgcggcac
     7081 tcgacggcgc ggcgcgccgt tgggctcggt cgtcgcctgg tcgaggcatt cctcgacctg
     7141 attgtccgga atcgcgaagg cgccgatcat gatcggcgtc gcttccgctt caaccgacac
     7201 gatccgcgcg tggttgagcg ggcagccgcc gttctcgtcg ctcagccaca cgaacgcggg
     7261 ccggcctttc gcgttgccgc gcacccgcgc cggacgattg ggcacgaaca ccgcactgtg
     7321 cgtgccggtc ggcagcgcgt tgaccggagg atagacgtcg aacctcgagc gagtgagcaa
     7381 cacaagataa cggctggcct tcgtttcggg tgacgcggtc gagatcagaa acgctttcag
     7441 tccaggatcg ttgttcacca ccatgtgcag gcagtcgttg gccagcgccg gcagcttcgc
     7501 ggcatcgatg gcgccgcccg cggttgcaac gatcgtcgag cgctggtggc atccggccgt
     7561 accggcggcg agttgcgcat cggttgcgcg ggcgatcgct tccgagtcga gcacgtagaa
     7621 gcccgcagtc gtaccggcga cgtagacgcg ctcgccgtcg tcggtgaccg acaccgagtg
     7681 gaggcggttc tgctcgcggt tctggaagtt gccggcttga ccaaggcggt tcttcgaatc
     7741 gctgaagtcg aggaaccggc cgtcagagaa cagccccgtg gcgtcgggac gctcgtcaat
     7801 cggtggaccg ccgacgtcct gcaacgtgaa gccggcgagc attctggcct tgggcagcag
     7861 cgctccggtg cgttcgtcgg tcaccgccag gacgatcaga tcgggtacgg tcaggccggg
     7921 gcgctccgga tcgggcaggc cgcccgtcca gttcgtcatg tagaccagca cgcgattgga
     7981 gttggccggg tcgtgccaga ggtagaactc gtgcgactgc gaatggaagt cggtgacttc
     8041 gctcgtgaac gtcggcaggc acgtgttcat gtcaatctga tacgactgca tccggccgag
     8101 cgtgttcgtg ccggcgttgc gcaccatgat gtagcggtct tcgccgctgg atgtcttgaa
     8161 gatcagggct cgaagttccc tgtcgtcgaa accctgattc ccctccgtca tggccgggat
     8221 ctctccgacc tgcagcggag gctgcatgtc cgggttgggt tgaatcctga agatgttgat
     8281 cgcgcgtctc acgcccgcgc cgttggcgtg accgacgaac aggcactgcc cggcgacgcc
     8341 cagcccgcgt ccatcgcctg caccatcgag atcgccaccc ctccacgcgc cggtcgccgc
     8401 gtcgcgaacc gacggcacgg tgaccaccca gtgcaggtgc tcgaaattcc ggaagaggtt
     8461 gagccgcttc tcgagctcga acgcgccggc cttcgcatcc gcttcagacg aggtgggatc
     8521 gatctggccc agacggttga cacggaccga cggcatctgc gccagaggcg tgtcgaaggt
     8581 gctgggctga catccggcgg gatctttcga gtgatcgacg cactgcgcgt cgaaggcgtc
     8641 gagcggtcgt atcgcgacag ccgacaggca caacacgcca agcctcacga gtcttcccat
     8701 cgtcatcggg tgctcctggc gaacgcgggc acagtatagc tacgatacag gcgcgccgac
     8761 gccacgagcc caggcgcacc tggcgcagat caccggacgg gaggccccat gcagcgatgc
     8821 acgcgactcg ggtttgccgc aatcctcgtc agcctcgcgg ccgtgtcgag tcacgtctcc
     8881 gcccagaggc aggtgacgat ggtcacgcga ctttacacag gtactgacgg tcagtcgcac
     8941 gccgaggaca tcgaggtcgc atggcggccg gccaagctac gggcggagtt gagcgattcg
     9001 gaatcggtac gagtcacttc agcgaacttt ctccgctggc cgcgcggctt cgtgtgggcg
     9061 ggccacccgg cgtcgaggcg ccagtacgtc gtcatcgtca gcggacgcgg cgaggtggac
     9121 gtcggcggcg ggcggaaggt ccagcttgct ccgggacggg tcctacttgc agaggacacg
     9181 acggggaagg ggcacacgac tcgagtcggg cccgacgaag atctcgtgat gctcctggtg
     9241 ccgctcgcgc aagagccttg atcaactacc ctagggctcg aagggaggac tcacatgcgc
     9301 agtcgagacc gagtgctgcc aatcgtcgtc acgatctccg cctttttcgc ctctcacgcc
     9361 ttcggggcgc aggcggcgcg cgacatcaag accacgaggg acgtcaaggt catctcgagc
     9421 gccggcgcgc gtgcgctggc cgacgcctgc accgtgtggg ccgagcagaa caagcaggtc
     9481 gtcgcgatgg cggtcctcga ctggggcggc aacctcatcg aattccacgc catggagggc
     9541 gcgacgatga acgccatcga cacggctctg ctcaaggcga agtcggcgct gcgctggcga
     9601 cggccgacat cggagaccaa caagatggtg cgaagcggag agaatctcgc gccgaccttc
     9661 atgaacgact tcccccagcc cggcgcactg cccatcgtcc tcggcggcca ggtcattggc
     9721 gccatgggtg tgtcaggcgc cgacggcgag aagtgcgcgc aggcggccat cgacgcggtg
     9781 ttcaaagggc aggcgacgac gacggccaga taaaggagat gaccacggcg gcccgtggga
     9841 ggagggttga gaatgactcg gacaagcgca ctcgtggtcg tcctgatctc ggttgcagca
     9901 tccgcctcgg cacagaccgc caagccggca gccgaaagcg atgccatcgc cgtactcacg
     9961 cgcgagattc gcgcgctgcg cgaggagatc accgagacgg cgcgggccgg cctcaggctg
    10021 cagctgctga ccgcgcgcat ccaggcgcag gagcagcgga tcatctacct cgatcggcag
    10081 cgcgccgagg cggtcacacg tcgtgccagc gccgagcagg cccgcgatgc catcgcgacc
    10141 cagattcaga cgttcgccgg cggcgacacc tcgaagctca ccgcggagca acggcgcgag
    10201 atggagccga tgatcgccgt gttcaaaaag caagttgcga cgcacgactc gctcgtcgcg
    10261 cagctcgaga cggaggaaat cgacgccgcc aacgctctcg cccaggagca ggggcggtgg
    10321 gcggacctca atcagcggct cgaggatctg gaacgctcgc tgaaatag
//
LOCUS       DBNGSH010000774         3144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_255566, whole genome shotgun sequence.
ACCESSION   DBNGSH010000774 DBNGSH010000000
VERSION     DBNGSH010000774.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_255566"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="AC16CY_17790"
     CDS             complement(<1..596)
                     /locus_tag="AC16CY_17790"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase family protein"
                     /protein_id="HIR3355708.1"
                     /translation="MTHSGETSGSAAGLEVAQRWLPALLLLFIGSGCAALIYEVVWFQ
                     LLQLSIGSSAVSLGVLLGIYMGGMCLGSLLLPRYLNPRHHPLKVYAALEAGIGLFGVI
                     VLFAVPVVGRLYTEIAGTGQVNLVLRALVAGICLLPPTLLMGATLPAIARWVETTPKG
                     VSWLGFFYGGNLAGAVAGSLIAGFYLLRVYDMPTATAVA"
     gene            717..1481
                     /locus_tag="AC16CY_17795"
     CDS             717..1481
                     /locus_tag="AC16CY_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3355709.1"
                     /translation="MQTDTGRIADDRDRGMTARGPTRTRVLVVEDEQDIAELIKHSLE
                     KGGDIEVEVSLTGDGALKAVTERAPDLIVLDLNLPVLSGLEVCRIMRSRANTSRVPII
                     MLTARTSESDRVTGLDVGADDYITKPFSLRELAARVRAVLRRGRAEPASGARDAEALV
                     YRGKHLVADFDAVAVSVDGAPVRLTRREFELLRYLVANRNRVLSRDRLLERVWGYDRL
                     VETRSVDVHVGRLRGKLGEAGQQIETVVGLGYRFVE"
     gene            complement(1492..1701)
                     /locus_tag="AC16CY_17800"
     CDS             complement(1492..1701)
                     /locus_tag="AC16CY_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355710.1"
                     /translation="MYARVLEKAGLVRRFIITSAGSAGWEVREERDSLVVRRTRYTDW
                     HRVERARMAFAHEADSLASAGWKES"
     gene            complement(1766..2659)
                     /locus_tag="AC16CY_17805"
     CDS             complement(1766..2659)
                     /locus_tag="AC16CY_17805"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3355711.1"
                     /translation="MKPRAAIIINPISGEGRRRDLARVRAEHAAALADAHEIDAEIIV
                     TERQGHARELAEAARHRGVTLFLAWGGDGTVNEVASAIVHSDASLAIVPSGSGNGLAR
                     ELGIPFDPAAAFGIAFEGRARQIDAGELDGRWFFNLAGIGLDARVAHRFAEGGLRRRG
                     FVRYLELAARELLTFQPENLTIRTDAATTSTSPLLVAIANARQYGNGALIAPQARVDD
                     GELDLVIVDNRSVWAVMRQVRRLFSGELARVPGVSMTRITTATISCALPVAYHVDGEP
                     HVGGVSVNARVRPRALRVQTA"
     gene            complement(2722..>3144)
                     /locus_tag="AC16CY_17810"
     CDS             complement(2722..>3144)
                     /locus_tag="AC16CY_17810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR3355712.1"
                     /translation="PLTERGIARFKEGVAGLGRLDAVIDEILTSPLVRARQTADLLSA
                     GLDSKPPVKVLDALAPGHEPDQIMALLARTAKRRRIALVGHEPGLGELAAHLIGARRP
                     LAFRKGGVCRIDLVSLASRRPGALIWLVTPKMLRHVGA"
BASE COUNT          567 a         1061 c         1031 g          485 t
ORIGIN      
        1 gcgacggccg tcgccgtcgg catgtcgtat acgcgcagca gataaaagcc ggcgataagg
       61 ctgccggcga ccgctcccgc caggttgccc ccgtagaaga agccgagcca ggacacgcct
      121 ttcggcgtcg tctccaccca ccgtgcaatg gctggaagcg tggcccccat caacagggtc
      181 ggcggcagca ggcagatgcc ggcgacgagt gcgcgaagta ccaggttgac ctggcccgtt
      241 ccggctatct cggtgtagag gcggccgacg accggtacgg cgaagaggac aatcaccccg
      301 aagaggccaa tgcccgcctc gagggccgca tacaccttca aggggtggtg ccgggggttc
      361 aagtacctcg gcagcaggag gctcccgagg cacatgccgc ccatgtagat gcccagcagc
      421 accccgagcg atacggccga ggagccgatg gaaagctgga gcagttgaaa ccagacgact
      481 tcgtagatga gggccgcgca gccagacccg ataaacagca gtaaaagcgc cgggagccag
      541 cgctgggcca cctcaaggcc ggctgcggac cccgaggtct ctccggaatg tgtcatcgcc
      601 tggcgatcct acttcaacag gcggatacct ccgtaaccca gatttaactt ccgtgtcacg
      661 ccccggaaac tcgcgttgca taccgtgact gctgcacgcg cagaaatggg gcttcagtgc
      721 agacagacac aggccggatc gcagacgatc gcgaccgtgg catgaccgcg cgcggaccca
      781 cccgcacgcg ggtcctcgtt gtcgaggacg agcaggacat cgccgaactc atcaagcaca
      841 gcctcgaaaa ggggggcgac atcgaggtcg aagtctccct gacgggcgac ggtgcgctca
      901 aggcagtcac cgagcgtgcg ccggacctga tcgtgctcga cctgaatctt ccggtgctca
      961 gcggactcga ggtctgccgg atcatgcgat cgcgcgccaa cacgtcgcga gtgccgatca
     1021 tcatgctgac ggcccggacg agcgaaagcg accgcgtgac cggcctcgac gtcggggccg
     1081 acgactacat cacaaagccg ttcagcctgc gggagcttgc cgcgcgggtc cgcgcggtgt
     1141 tgcggcgcgg acgggccgaa cccgcatccg gcgcgcgcga cgccgaagcg ctcgtctacc
     1201 ggggcaagca tctcgttgcc gatttcgacg cggtggccgt cagcgtcgat ggagcgcccg
     1261 tccggctgac gcgccgcgag ttcgagctac tccgctacct ggtcgccaac cgcaatcgcg
     1321 tgctgtcgcg cgatcgtctg ctcgaacgtg tgtggggcta cgaccggctg gtcgagacga
     1381 gatccgtcga tgtgcatgtc ggccgcttgc gcggcaagct gggggaagcc ggccagcaga
     1441 tcgaaacggt cgtggggttg ggctatcgat tcgtcgagta gatctcgtag gtcaggattc
     1501 tttccatccg gcactggcaa gcgagtcggc ttcgtgcgcg aacgccatgc gggcgcgctc
     1561 cacacgatgc cagtcggtgt aacgcgtccg gcgcaccacc aggctgtccc gctcctcccg
     1621 tacctcccag ccggcgctcc ctgcgctggt gatgatgaag cgccggacca agcctgcctt
     1681 ttccaggacg cgagcgtaca tagggctcga tctatacctc gatcggaaag gccggcgctg
     1741 taaacattgt gtaaactcgg ctttcttacg ccgtttgcac gcgcagcgcg cgagggcgaa
     1801 cgcgcgcatt gacggagaca ccgcccacgt gaggctctcc gtccacatgg tatgccacgg
     1861 gcaatgcgca cgagatggtc gccgtcgtga tccgcgtcat cgacacgccc ggcacacgcg
     1921 ccagttctcc cgaaaacagt ctccgcacct gacgcatcac ggcccacacc gaacggttat
     1981 ctacaatgac gaggtcgagt tccccgtcgt cgacccgtgc ctggggcgcg atgagcgctc
     2041 cgttgccata ttgccgggca ttggcgatcg cgaccagcag cgggctcgtg gacgtcgtcg
     2101 cggcgtcggt ccggatggtc aggttctccg gctggaaggt caacagctca cgagccgcca
     2161 attcaaggta cctgacaaag ccgcgacgcc gcaaaccgcc ctcggcgaac cgatgcgcga
     2221 cgcgtgcgtc gagcccgatg ccagccaggt tgaagaacca ccggccgtcg agctcgcccg
     2281 cgtcgatctg ccgggcgcgt ccctcgaagg cgatcccgaa ggccgccgcc gggtcgaaag
     2341 ggatgccgag ctcccgcgcc agcccgttgc cggatccgct gggcacgatc gccagcgacg
     2401 cgtcggaatg aacgatcgcc gaggcgacct cgttgaccgt cccgtcgccg ccccatgcga
     2461 ggaagagcgt cacgccacgg tggcgcgcgg cctcggccag ctccctggcg tgcccctgac
     2521 gctcggtgac gatgatctcg gcgtcaatct cgtgcgcgtc ggcaagcgca gccgcgtgct
     2581 ccgcacggac gcgagccagg tcgcggcgcc ggccctcgcc tgaaatcggg ttgatgatga
     2641 tggcagcccg cggcttcata ccgggtcaga tcttgattct tgcaatcggc cgcacgggcg
     2701 caaagatcaa gatctgaccc ctcacgcgcc cacgtggcgc agcatcttgg gcgtcaccag
     2761 ccagatgagc gctccgggcc gcctgcttgc gaggctcacg aggtcgatgc ggcagacacc
     2821 cccttttcga aaagcgagcg gacgccgcgc gccgatgaga tgggcggcca gctcccccag
     2881 ccccggttcg tggccgacga gggcgatgcg ccggcgcttc gcggttcgcg ccagcagcgc
     2941 catgatctga tcgggctcgt ggccgggcgc aagcgcgtcg agcactttga ctggcggttt
     3001 cgagtccaga ccggcagaca gcaggtcggc cgtttgtctc gcgcgcacga gaggactcgt
     3061 gaggatctcg tcgatgacgg cgtcgagcct gcccagacct gcaacgccct ccttgaagcg
     3121 cgcaatgccg cgctcggtca acgg
//
LOCUS       DBNGSH010000775         1844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3991093, whole genome shotgun sequence.
ACCESSION   DBNGSH010000775 DBNGSH010000000
VERSION     DBNGSH010000775.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3991093"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1129
                     /locus_tag="AC16CY_17815"
     CDS             191..1129
                     /locus_tag="AC16CY_17815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3355713.1"
                     /translation="MKPSRRYTILIADRTTGVVRRVTVAARPAVAFACAMVTLPLLVG
                     LGAAWKARSDVANLYASEHALAQENASYRTATAELSGQIQSLQAAISDLDAKAALDPS
                     LARAMEGLPGLVKSRAMGGGVASPSRQETRYASAALTALANPEDTFGLIRTVLEGLES
                     SLHFVQRNVERRNALADSTPSIWPAHGWISSSMGERSDPMTGAADYHAGLDIVGDRGQ
                     PVYATAAGVVTFAGRQGAYGNLVEIDHGFELKTRYGHLSRFQVNQGDRVTRGDLIGNL
                     GATGRATGTHLHYEVLANGRLLNPLRLLSQQKPRDR"
     gene            1220..>1844
                     /locus_tag="AC16CY_17820"
     CDS             1220..>1844
                     /locus_tag="AC16CY_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3355714.1"
                     /translation="MIGQLLAKVIGTQNEREIKRLRPRVAEINALEPSIQSLSDEQLR
                     GKTEEFRRRVADGATLEELQAEAFAVVREAGRRVLNMRHFDVQLIGGMVLHNGTIAEM
                     KTGEGKTLVATLPAYLNALEGKGVHVVTVNDYLARRDSEWMGRLYRFLGMSVGVIQHD
                     LKDDQRQVAYGADITYGTNNEFGFDYLRDNMKFELSQMVQRGHHYAIV"
BASE COUNT          319 a          620 c          570 g          335 t
ORIGIN      
        1 ttgtcggaca ttcgcctgat gatcgctcac gacttctgtc gttgaggacg cttttcttcc
       61 tgtgataggc tcgggatgtc gggccctccc cccgtcgttt gtcagcatcc gatagtacgg
      121 ttgttcaagc tcggacctgg cagtgtgctg gatccggtag tcgaggcgtg cattcgaaca
      181 ggtattgctg ttgaagccat cccgtcgtta cacgatcctc atcgctgacc gcacgaccgg
      241 cgtcgtcaga cgcgtcacgg tcgccgctcg tccggcggtc gcctttgcct gcgccatggt
      301 cacgctcccc ttgctcgtgg gcctgggggc ggcgtggaag gccaggagcg acgtcgccaa
      361 tctctacgcg agcgagcacg cgctggctca ggaaaacgcc agttatcgta cggcaactgc
      421 cgagctttca ggccagattc aatctctgca ggcggccatt tccgatctcg acgccaaggc
      481 ggcgctcgat cccagccttg cgcgtgcgat ggaagggctg cccggcctcg tcaagtcgcg
      541 tgcgatgggc ggcggcgtcg cgagcccgtc ccgtcaggag accagatacg ccagcgccgc
      601 gctcacggca ctggccaatc ctgaagacac cttcggcctg attcgcacgg tgctcgaggg
      661 gctcgagtcc agccttcact tcgtgcagcg gaacgtcgaa cgccgcaacg cgctggccga
      721 ctccacgccg tcgatctggc ccgctcacgg gtggatctcc tcgtcgatgg gcgagcgctc
      781 tgatccgatg acgggcgccg cggactatca cgcgggcctc gacatcgtgg gcgaccgtgg
      841 tcagccggtg tacgccaccg cggccggcgt cgtcacgttc gcgggccggc agggggcata
      901 cggaaacctc gtcgagatcg atcacggttt cgagctcaag acacgatacg gccatctctc
      961 ccgcttccag gtcaatcagg gcgaccgcgt cacgcgcggc gatctgatcg gcaacctcgg
     1021 cgccactggc cgcgcgaccg gcacgcatct ccactacgag gtgctggcga acggccgact
     1081 cctcaatccg ctccgtctgc tctcgcagca gaagccgcgc gaccgctaag ttcctcgcaa
     1141 tctgtcgttt ccttgccgat tctgtggccg ctggctacaa tcggctgata tccactcgta
     1201 gtcagtccgc tccctacgca tgattggcca actgctcgcc aaagtcatcg gcacgcagaa
     1261 cgaacgcgag atcaagcgtc tccgcccgcg cgttgccgag atcaacgccc tcgagccctc
     1321 gattcagtcg ctctccgacg aacagcttcg cggaaagaca gaggagttcc ggcgacgggt
     1381 cgccgacggc gccacgctcg aggagttgca ggccgaagcc ttcgccgtcg ttcgcgaggc
     1441 cggacgccgt gtgctcaaca tgcggcactt cgacgtgcag ctcatcggcg gcatggtgct
     1501 gcacaacggc acgatcgcgg agatgaagac gggcgaaggc aagacgctgg tcgccacgct
     1561 gcccgcctac ctgaacgccc tcgaaggcaa gggcgtccac gtcgtgactg tgaacgacta
     1621 ccttgcgcgc cgcgactcgg agtggatggg gcgcttgtac cgcttcctcg gcatgagcgt
     1681 cggcgtcatc cagcacgatc tcaaggacga tcagcggcag gtggcctacg gcgccgacat
     1741 cacctacggc accaataacg agttcggctt cgactacctc cgcgacaaca tgaagttcga
     1801 gctgagccag atggtccagc gcgggcatca ctacgcgatc gtgg
//
LOCUS       DBNGSH010000776         6297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4330755, whole genome shotgun sequence.
ACCESSION   DBNGSH010000776 DBNGSH010000000
VERSION     DBNGSH010000776.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4330755"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="AC16CY_17825"
     CDS             <1..648
                     /locus_tag="AC16CY_17825"
                     /inference="COORDINATES: protein motif:HMM:NF018269.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HIR3355715.1"
                     /translation="SGQRRVGRFGWKAQHATLLSFGADAYRNEMGITNEVFPQEMAVG
                     VTADRMRVCDLIPEPEDRRDPRTGRRGIDNFASFMRLLAPLGRGPIDEAARSGEQIFA
                     AIGCATCHTPVLQTGPSSSPLFHHVPVPLFSDLLLHDIGTGDGIAQSVAAPEEIRTPG
                     LWGLRFRRPLLHDGSAATIEDAIRRHNGEADAARRGFDRAGAEAREALITFLRSL"
     gene            750..2399
                     /locus_tag="AC16CY_17830"
     CDS             750..2399
                     /locus_tag="AC16CY_17830"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR3355716.1"
                     /translation="MRGRLGAPIVTVAVAVATMSLAQAQKRDPVEKPGPPMSGFYDYW
                     NAGSEFGVGIGITDVLTCSIGVLTQPAVYAGNNLVDCDAEVPHNETTITVNPYDPSHA
                     VAGYHSYQISALGATLIAHIIGTTSVTFDGGANWQEVVPPITPFQFSGDPALAFDTNG
                     RVYFANIADHEGPGGNFTAPSVIVAHSDDGGLTWSSPTTVAAGQGAVTAGGGSRVLSF
                     QDKEFIAVDTSPASPYTDRAYVTWTSFQDTPFQSISPIMVARSDNGTSWSQPRRISGS
                     HPACATPFGGSAPGECAHNQFSSPSTAPNGKVYVAFENFNTPAENQYMVVTSSNGGAS
                     FGNPVRVDTIFDVNFPISPFSGRSTLTGCQFRVAAPGNIAADPSDPTGNTAYIAWADN
                     RNGTPAASNADVLLARTTDGGQNWTTYVIDNTPNDQFYAWVAVAPNGRVDVGYMDRSY
                     SSGQGVCQYGFSLNRLTFNAGGIATQVKTRVDTGLSDAGRSRWFSSLTGGNATFLGDY
                     NAVAVGSDGATWSLWTDFRNIIPMAPANRNHGQHAVGVRTP"
     gene            complement(2455..4665)
                     /locus_tag="AC16CY_17835"
     CDS             complement(2455..4665)
                     /locus_tag="AC16CY_17835"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3355717.1"
                     /translation="MKNAVKNVLTLTGIAVFAVAVVALGQGSQAANEALWEAARAGDT
                     AGITTALAQGADVNAKSRYNVTAIIFAAGSGRLDAVKLLVSRGADVNAQDSFYNARAA
                     DMALTNGHSSVALFLVEAGANADGLLATGVQLNNEALVKAAAAGKVSRQGLQAALGLA
                     TATKREALAAIVKASLDKLPPEPAPAAPAFKLDPATLPRYAGTYRDAASGLTMNVTVQ
                     DGGLLAQVVGQPAIRLVPSNQNVFRIVEVNATLTFNERGGLVESVALVQGPANLTMAR
                     VNPEAAAAPAAAAAAAPAPAAPAAPVAAVTRNTKPRNWPAFRGEGSAGNGDGQRAVTE
                     WDVATGKNIKWKTPIPGIAVSSPIVWGNRVFATTAIGKAGDNTFRTGLYGDVKPVEDL
                     SPHEFKIYSLDTATGKIVWERTAFSGAPKSKRHTKSSQASSTPATDGKRVVAVFGSAG
                     LLVAWDFEGKELWRVDLGALDSGWFFDPAFQWGHSSSPIIYRNSVIVQADIQKNSYIA
                     AWDLATGKQLWKTARADEISTWGTPVVARTADGRDEIITNGTKVRGYDPATGKQLWTL
                     GPNSEITIGTPALGNGLVFVTGGYPPVRPIYAIRPGAEGDISLAKGQESNQSIAWSNM
                     AEGTYIPTPIVYDGYLFTLNINGVISAYNPDTGQRAFRGRVGVAGSYSASPVGADGHL
                     YVASEDGEIYVVDASPGLKQVAKNDMKEVIMATPAISDGLIVVRTLGHLYGIGQ"
     gene            4803..6293
                     /locus_tag="AC16CY_17840"
     CDS             4803..6293
                     /locus_tag="AC16CY_17840"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR3355718.1"
                     /translation="MTAPSHTSPESRSGLDPWQSAELPEPPRPRGLGWLSAVGPGVIA
                     LGVSIGSGEFLLGPAAFVAHGLSLLWVVLVAITLQTIFNTEVMRYTLATGEPVFTGFM
                     RTRPSSTLWAWVYAALYFLQVGWPAWAGTAAAAIFFLFARRLAGTGTADATTIYVIAV
                     AAFLLCVVILMVGRRIERTLELLNWVLVVTILGGFFVLTLLFVPAGTWVEAAAGFGGF
                     DLPNGRFDFLPAGADFFLLGALVAYSGSGGVVNIVLSNWARDRGYGMGERAGYIPAAA
                     SGERGSVAHTGYHFPADARSMDRWRGWWRVVAADQWGLFWAGAVLGMLLPALLYVTFL
                     PRGTNIQGLGISAALASAIGSRHGAIIAGVIAFLGAWLLFKTQLDNVEGMVRAITDIL
                     WTGSRRVRAWRGGDVRAVYYSVLAMIVVWGLIAMQIAQPIVLLQLGANIAGLVFVFAA
                     PHLLYINTRLLPPHVRPPMWRRVALVVMTLFYGFFVALSFGSLWFD"
BASE COUNT          943 a         2121 c         2113 g         1120 t
ORIGIN      
        1 tccgggcaaa gaagagtcgg gcgcttcgga tggaaggcgc agcacgcgac gctgctctcg
       61 ttcggcgcgg acgcctatcg caacgaaatg gggatcacca acgaggtgtt cccgcaggag
      121 atggccgtcg gggtcaccgc ggaccggatg cgagtctgcg acctgattcc cgagcccgag
      181 gacaggaggg atccgaggac cgggcgccgc ggcatcgaca acttcgccag cttcatgcgg
      241 ctgctggccc cgctgggccg cgggccgatc gacgaggctg cccgcagcgg cgaacagatc
      301 tttgccgcga tcggctgcgc cacgtgccac acgccggtgc tgcagaccgg gccgagctcg
      361 agcccgctct ttcatcacgt cccggtcccg ctcttctcgg acttgctcct gcacgacatc
      421 gggacgggcg acggcatcgc gcaaagcgtc gcggcgccgg aagagatccg cacgccgggg
      481 ttatggggcc tccgcttccg ccgcccgctg ctgcacgacg gatcggccgc gacgatcgag
      541 gacgcgattc gacggcacaa cggcgaggcg gatgccgctc ggcgcgggtt cgaccgggcg
      601 ggcgccgagg ctcgggaagc gctgatcacg tttttgagat cgctgtaggt tgtagtcgaa
      661 ggcgcgcggg cgaggccggg cggatgtagg atcggggctt cggcggtccc cggcgcgtga
      721 cgcgcgccaa agagatactg gaggctccga tgagaggcag gcttggcgct ccgatcgtga
      781 cagtcgccgt tgccgttgca accatgtcgc tcgcgcaggc ccaaaaacgc gacccggtcg
      841 agaagccggg tccaccgatg tcgggcttct acgattactg gaacgccggc agcgagttcg
      901 gcgtgggcat cggaatcacc gacgtgctta cctgctcgat cggcgttctg acccagcccg
      961 cggtctacgc cggcaacaat ctcgtggact gcgacgccga agtgccgcac aacgagacca
     1021 cgatcacggt caatccctac gatccgtcgc acgcggtggc gggctaccac tcgtatcaga
     1081 tcagcgctct cggggccact ctcatcgcgc acatcatcgg gacgacgtcg gtgaccttcg
     1141 acgggggcgc caactggcag gaagtcgtgc ctcccattac gccgttccag ttcagcggcg
     1201 atccggctct ggctttcgac acgaacggcc gcgtctactt cgcgaacatc gccgaccacg
     1261 aagggccggg cggcaacttc accgcgccaa gtgtcatcgt ggcgcattcc gacgacggcg
     1321 ggctgacgtg gtcgtccccg acgacggtcg ccgccggcca gggcgccgtg accgcgggcg
     1381 gcggcagccg cgtgctgtcc tttcaggaca aggagttcat tgcggtcgat acctcacccg
     1441 ccagcccgta caccgatcgc gcctacgtga cgtggacgag ctttcaggac acgccgtttc
     1501 aatcgatctc accgatcatg gtggcacgca gcgacaatgg aacgagctgg tcgcagccga
     1561 ggcggatcag cggctcgcat ccggcatgcg caacgccgtt cggcggcagc gcgcccggcg
     1621 agtgcgcaca caatcagttc tcgagtccga gcacggcgcc gaacgggaag gtgtacgtgg
     1681 ccttcgagaa cttcaacact ccggcagaaa accagtacat ggtggtcacc tcgtccaacg
     1741 gtggcgcgtc gttcggcaat cccgtccgcg tggacacaat cttcgacgtg aactttccca
     1801 tcagcccgtt cagtggccgg agcacgctca ccggatgcca gttccgcgta gccgccccgg
     1861 gcaacatcgc ggccgatccg agcgatccga cggggaatac tgcctacatc gcctgggccg
     1921 acaaccgcaa cggcacgccg gcagcatcca atgccgacgt tctgctggcc aggaccactg
     1981 acggcggaca gaactggacc acgtacgtca tcgacaacac gccgaacgat cagttctacg
     2041 cctgggtggc cgtggcgccc aatggccgtg tggacgtggg ctacatggac cgctcctact
     2101 cctcgggcca gggcgtatgt cagtacgggt tcagcctgaa ccgcttgacg ttcaacgcgg
     2161 gcgggattgc cacgcaggtg aagacccgcg tggacaccgg cctctcggat gcggggcgct
     2221 cgcggtggtt ctcgagcctg actggcggca acgcgacgtt cctgggcgac tacaatgccg
     2281 tggccgttgg cagcgatggc gccacctgga gcctctggac cgacttccgg aacatcattc
     2341 cgatggcgcc ggcgaaccgg aaccacgggc agcacgccgt gggcgttcgc acgccctgag
     2401 cggcggaagt gacggaggac gcattcgcac gcgcgtgcgg cgcccgcgtg acgtctactg
     2461 ccctatgccg tacagatggc cgagcgtgcg caccacgatg aggccgtcgg agatcgcggg
     2521 cgtcgccatg atcacctctt tcatgtcgtt cttggcgacc tgcttcagac ccgggcttgc
     2581 gtccacgaca tagatctcgc cgtcctcgct cgcgacgtac aaatggccgt cggcgccgac
     2641 cggtgacgcc gagtacgagc cggccactcc gacacggccg cggaacgccc gctgccccgt
     2701 gtcggggttg tacgccgaga tgacgccgtt gatgttgagc gtgaacaggt acccgtcgta
     2761 cacgatcggc gtgggaatgt aggtcccttc cgccatgttg ctccacgcga tcgactggtt
     2821 cgactcctgc cccttcgcca gcgagatgtc gccctcggcc cccggccgga ttgcgtagat
     2881 cggccgcacc ggcggatagc cgcccgtcac gaacacgagg ccgttgccga gagccggcgt
     2941 cccgatcgtg atctcggagt tgggtccgag cgtccacagc tgcttgcccg tggcagggtc
     3001 gtagccgcgg accttcgtcc cgttggtgat gatctcgtcg cggccgtcgg ccgtgcgggc
     3061 gacgaccggc gtgccccacg tcgagatttc gtcggcgcgc gccgtcttcc agagctgctt
     3121 gccggtggcg agatcccacg cggcaatgta ggagttcttc tggatgtcgg cctgcacgat
     3181 gaccgagttc cgatagatga tcggcgagct cgagtgtccc cactggaagg cgggatcgaa
     3241 gaaccatccg ctgtcgagtg cgccgaggtc cacgcgccac agctccttgc cttcgaagtc
     3301 ccacgcgacg agcagaccgg ccgacccgaa gacggcaact acccgcttgc cgtcggttgc
     3361 gggcgttgag ctcgcctggc tgctcttcgt atgccgcttg ctcttcggcg cgcccgagaa
     3421 cgcggtgcgc tcccacacga tcttccccgt ggctgtgtcg aggctgtaga tcttgaactc
     3481 gtgcggggag agatcctcca ccggcttcac gtctccatac agccctgtcc tgaacgtgtt
     3541 gtcgcctgcc ttgccgatcg cggtggtcgc gaatacccga ttgccccaca cgatggggct
     3601 cgagacggcg atgccgggga tcggcgtctt ccacttgatg ttcttgccgg tcgccacatc
     3661 ccattcggtg acggcgcgct gtccgtcgcc gttgcccgcg ctcccctcgc ctcgaaacgc
     3721 cggccagttg cgcggcttcg tgttgcgcgt cacggctgcc acgggtgcgg cgggtgccgc
     3781 cggcgcgggc gcagcagcgg cagccgctgc cggagccgcg gccgcttcag ggttcacgcg
     3841 ggccatcgtc aggttcgcgg gaccctggac gagcgcgacc gactccacga ggccaccgcg
     3901 ctcgttgaac gtgagcgtgg cgttgacctc gacgatgcga aacacattct ggttggaggg
     3961 gacgaggcgg attgccggct gccccacgac ctgcgccaac aggcctccgt cctgcaccgt
     4021 gacgttcatc gtcaggccgc tcgctgcgtc gcggtacgtc ccggcgtacc tgggcagggt
     4081 tgcagggtcg agcttgaacg ccggtgcggc cggcgcgggc tcgggcggaa gcttgtcgag
     4141 cgacgccttg acgatcgcgg cgagcgcctc gcgcttggtc gcagtcgcga gaccgagggc
     4201 agcctggagc ccctggcgcg acaccttgcc ggcggcggcc gccttgacga gcgcttcgtt
     4261 gttcaactgg acgccggtcg ccagcagccc gtcggcgttt gcgccagcct cgacgagaaa
     4321 gagcgcgacg ctgctgtggc cgttggtcag cgccatgtcg gcggcgcgcg cgttgtagaa
     4381 gctgtcctgc gcgttgacgt cggcgcctcg cgagacgagc agcttcacgg cgtcgagccg
     4441 tccgctgccg gccgcgaaga tgatcgccgt aacgttgtat cgcgacttcg cgttgacgtc
     4501 ggcgccctgg gcgagcgccg tcgtgatgcc cgccgtgtcg ccggcgcgag cggcctccca
     4561 gagcgcctcg tttgcagcct gggatccttg accgagggcc accacagcaa cagcgaacac
     4621 cgcgataccg gtaagcgtca ggacgttctt cacagcgttc ttcacgacgc gcctccctgt
     4681 gcaggggtca gaccccttca cgtgtttgca accgtgcaac cgcattagtc tacgggatac
     4741 ccgcgggccg tgttccgcga cgccgccgta taatcctgcg tcccttgata gtcccgcgtc
     4801 ccttgacggc cccgtcacat acatcacccg agtcccgctc cgggctcgat ccctggcaat
     4861 ccgcagagct gcccgaacca ccgagaccac gggggctggg gtggctgtcc gccgtcggtc
     4921 cgggcgtcat cgcgctcggc gtctcgatcg gcagcggcga gttcctcctc gggccggcag
     4981 ccttcgtggc gcacggactg tcgctcctgt gggtggtcct ggtcgccatc acgctccaga
     5041 cgatcttcaa caccgaggtg atgcgataca cgctggcgac cggcgagccg gtgttcacgg
     5101 gctttatgcg cacgcggccc tcgtccacgc tgtgggcgtg ggtctatgcg gcactgtatt
     5161 tcctgcaggt gggctggccg gcgtgggcgg gcactgctgc ggccgcgatc ttcttcctgt
     5221 tcgcccggcg gcttgccggc accggcacgg ccgacgcgac gaccatctac gtcatcgcgg
     5281 ttgccgcgtt tctgctgtgc gtggtgattc tcatggtggg ccggcgcatc gagcgcacgc
     5341 tcgagctcct caactgggtg ctcgtggtca ccatcctggg aggcttcttc gtgctcacgc
     5401 tcctgttcgt cccggcgggc acgtgggtgg aggcggcagc cggctttggc ggcttcgacc
     5461 tgccgaacgg gcgattcgat tttcttccag cgggtgcgga tttctttctc ctcggcgcgc
     5521 tcgtcgccta ctcgggctct ggcggcgtgg tgaacatcgt gctgtcgaac tgggcgcgcg
     5581 accgtggcta cggcatgggt gagcgcgccg gatacattcc ggccgccgcg tcgggcgaac
     5641 gaggcagcgt cgcgcacacg ggctatcact tccctgccga cgcacgctcg atggatcggt
     5701 ggcgtggatg gtggcgcgtc gtcgccgctg atcaatgggg gttgttctgg gcgggagcag
     5761 ttctgggaat gctgctgccg gcgcttctct acgtgacctt cctgccgcgc ggcacgaaca
     5821 ttcagggtct cggcatcagc gcggcgctcg cctcggctat cggcagccgg catggtgcca
     5881 tcatcgccgg cgtcatagcg ttcctcggcg cctggctgct cttcaagacg cagctcgaca
     5941 acgtcgaagg catggttcga gcgatcaccg acattctctg gaccggcagc cggcgcgtgc
     6001 gggcgtggcg cgggggcgac gtgcgcgcgg tgtattacag cgtgctcgcg atgatcgtcg
     6061 tctgggggct gattgccatg cagatcgcgc agccgatcgt gctcctgcag ctcggcgcca
     6121 acatcgcggg cctcgtcttc gtttttgccg cgccgcacct gctgtacatc aacacaaggc
     6181 tcctgccgcc gcacgtgcgg ccgccgatgt ggcggcgggt cgcgctcgtc gtgatgacgc
     6241 tcttctacgg attcttcgtc gcgctctcgt tcggaagcct gtggtttgac tgaacgc
//
LOCUS       DBNGSH010000777         7214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3651856, whole genome shotgun sequence.
ACCESSION   DBNGSH010000777 DBNGSH010000000
VERSION     DBNGSH010000777.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3651856"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="AC16CY_17845"
     CDS             complement(<1..515)
                     /locus_tag="AC16CY_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355719.1"
                     /translation="MINYTERLWQLMSDIVERVPTLSFLDMSRVLVFARAGRADTEGP
                     YATCHCVCLPPSEPGYYYWRDRATGELTRRSEWFVTKSPIVRVGAVGIDYMVSFTLPR
                     FCDQRWVSARKRSDYGNDPDWMIKLDTVVHELYHIDPDKPGIRRIERADGRYSANCHA
                     GRFFEDVVAMVR"
     gene            complement(637..1446)
                     /gene="mtgA"
                     /locus_tag="AC16CY_17850"
     CDS             complement(637..1446)
                     /gene="mtgA"
                     /locus_tag="AC16CY_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR3355720.1"
                     /translation="MRKVPPRVRRALIALAGVGFGVTCYVYLTLPDVRPLARSNPTST
                     AFMQLREREAARQGRKIRRVHQWVPYSRISPNLRRAVLVAEDGAFWDHEGIDVAEIRK
                     SIEDSVARRQAPRGASTITQQLAKNLYLSPSYDPLRKLRELIIARRLEVALSKARIFE
                     IYLNVIEWGDGVWGAEAASRTYFGIPASALSPSQAALLAGAIINPRVLSPARPQRRLM
                     RRQRIILSRMGAVDSFAVSPAEPDIEPPRFPTTVDQEEAQQGEEPEEAQPD"
     gene            complement(1443..2615)
                     /locus_tag="AC16CY_17855"
     CDS             complement(1443..2615)
                     /locus_tag="AC16CY_17855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3355721.1"
                     /translation="MPIPLPSTERILLGPGPSLIAPRVMRAMAAPVLSHLDPEFVPML
                     DEIRASLQRVFRADGRALTLATSGTGTSAMEAAVANAVRDGTRGVVVVTGYFGDRLAQ
                     MFQRYGAAVRRIDVEWGRAVDPQRLRDELRLEGADVVGIVHAETSTGVRNPVRELASV
                     AREHGALIIADMVTSLGGHEVDLAGWGVDIAYSCTQKCIGAPSGLAPLAVSGPARERL
                     VPCRSFYLDLKLLEDYWVGRKYHHTMSSTLIYALREALLMVEEEGLEARQARHERHHR
                     ALVAGIEAMGLSMLPPESERLWTLNAVRLPAGVDEGAVRRTMLTTYNIEVGAGLGPLA
                     GKIWRVGLMGASSTPQTVLQFLAACEGALSSHGYNVPEGAGAAAALAALRSTLVAP"
     gene            complement(2590..3567)
                     /locus_tag="AC16CY_17860"
     CDS             complement(2590..3567)
                     /locus_tag="AC16CY_17860"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3355722.1"
                     /translation="MKRFPGIFTPIATPFRSDDTLDEAGLRSNTARWMKTSLRGLVVL
                     GSNAEAPQLEDDEADRIVDIVRAGVPSDRPLIAGTGRESTKATIAASKRAAAAGADAV
                     LVRTPSFFKPQMTTEAFVRFYTDVAEASPVPVLLYNVSMYTGVNLLPDAVERLAVHPN
                     IVGIKESGSDIGQIAEFVSRTPDDFTVLAGSAATLFHALCAGCDGAILALASLVPDEC
                     NEMMTLVHDNRLVEARLLQRRLLPLARSVGGTYGVPGLKAALHLIGYEAGPPRPPLRP
                     VPTSVIDTLRDQLDALGVLPHARAEARALRDSTRSIHRHNTDAHTASLD"
     gene            complement(3564..4538)
                     /locus_tag="AC16CY_17865"
     CDS             complement(3564..4538)
                     /locus_tag="AC16CY_17865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355723.1"
                     /translation="MKQVAWLLLGLVAIAALGAPWLAPNQPDRRFDDLLYAPPTRIRV
                     LDEGLRAPFIHPWRVVSRLERRFEPVVSERATLRWFADGTLVTGRPDDGAPLLLLGAD
                     GYGRDMFSRLLHGARLTLALSVLATLLATLAGAAIGGLAGYAGGWADAILSRVTEFVL
                     VLPAIYVALALRAALPLVLPDGAVFFLLLLIFAALGWPVVARGVRAIVAAERSRDYAS
                     AARAAGASDLRVLIRHLLPASWGYLKAQGTLLLPAFILAEATLSYIGLGFASTTPTWG
                     TMLQDASNVALLGDAPWTLAPAAAIFIVVLGVNLLAQGGGRVPVQLER"
     gene            complement(4596..5555)
                     /locus_tag="AC16CY_17870"
     CDS             complement(4596..5555)
                     /locus_tag="AC16CY_17870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355724.1"
                     /translation="MARFFLRRLLFAIVLVAVASSVALLLARLAPGDMTAQLGPFASR
                     VDVESTRARFDLDRSPAAQWWLWVRRAVRFDFGDSFLYGRPVGPLVAKAAGNTALLGI
                     VSLVAATLIGISLGVFTGSRGEGVPASIVRGASLVVLSMPPLVTSLLFVFVAARTGWL
                     PSGSMTSSGAAAASWPAWIADVLWHLPLPVLALSLPIAATFERLQSQAMGEAVRQPFV
                     LAAVARGAPRRLVILRHAWPVSLRSVSAVYGLAIGALLSGSFIVEYVTTWPGLGRLMF
                     DALRARDIYLVAACAAAGAGFLALGTAAGDVLLAAADPRVRHE"
     gene            complement(5670..>7214)
                     /locus_tag="AC16CY_17875"
     CDS             complement(5670..>7214)
                     /locus_tag="AC16CY_17875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3355725.1"
                     /translation="TSDTISTLMHGRLVRINRSTFELEPWLAERWESSSDGRTHTLHL
                     RPGVVWSDGTPFTSADVLFSIQAVYDPRVKSVVASSLTVGGRPITASAPDAATVVIGF
                     ASPAGPGLRLLDMLPILPRHALEPALQDGTLARAWSTATPPSDIVGTGPFLLREYQPG
                     QRLVFDRNPRYWRRAPNGDQLPYLDRLVIEIVPDQNAELLRLQSGASDLTHSELRSDD
                     YIPVRRAEQEGRLTMIELGVTADADAFWFCLKPEVKKKDRRFAFVQKREFRQAISHAV
                     DREAFARTVFLGEAVPVWGPITPGNKVWFSPNVPRYPHDLARARQLLASIGLEDRNGN
                     GVVEDASGAEARFAVITQQGIGYYERGTTVLREQVAAAGIALDIVPMEFSAMIKRLLA
                     CDYDAIYMRPLATELDPAGNLDYWLSSGGAHLWNMQQKAPATDWEKSIDALMHEQAAT
                     LDDARRHELFNEVQATLAREAPVLYFAAPRLYYAHSRRVRGMVPSITRPPVLWNADSL
                     SVAGGH"
BASE COUNT         1200 a         2475 c         2516 g         1023 t
ORIGIN      
        1 cgcaccatcg cgacgacgtc ctcgaagaag cggccggcgt ggcagttggc ggagtacctg
       61 ccatcggccc gctcgatgcg gcggattccg ggcttgtcgg gatcgatgtg gtacagctcg
      121 tggaccaccg tgtcgagctt gatcatccag tccggatcgt tcccgtaatc ggatcgcttg
      181 cgcgcgctca cccatcgctg gtcgcagaac cgcggcagcg tgaacgacac catgtagtcg
      241 atgccgaccg cgccgacgcg gacgatcggc gacttggtga cgaaccactc cgatcggcgg
      301 gtcagctccc cagttgcccg atcccgccag tagtagtacc ccggctcgct cggtggaagg
      361 cagacgcagt ggcaggtcgc gtacggtcct tcggtgtcag cccgtccggc gcgcgcgaaa
      421 accagcactc ggctcatatc cagaaatgac agtgtcggca cgcgctcgac gatgtcggac
      481 atgagttgcc acaaacgctc cgtatagttg atcatccgct ccgcttttcc gacagccgct
      541 tctctactgc actaacacgt ttgacagtcg cttttccgga gtttcggccg ctccctcccc
      601 aaccttcact cgacaataaa atcgtgcacg ggcgtgctag tccggctggg cctcttccgg
      661 ctcctcgcct tgctgcgcct cctcctggtc cacggtcgtg gggaaccgag gcggctcgat
      721 gtcgggttcg gcgggagaga cggcaaacga atcgacggcc cccatcctgc tcaggatgat
      781 ccgctgccgg cgcatcaggc ggcgctgcgg acgcgcgggg ctcaacacgc gcgggttgat
      841 gatcgcgccc gccagcagag cggcctgcga cggcgacaac gcgctggcgg ggattccaaa
      901 gtaggtccgc gacgccgcct ccgcgcccca caccccgtcg ccccattcga tgacgttgag
      961 atagatctcg aagatacggg ccttcgacag cgcgacctcg agacgcctcg cgatgatgag
     1021 ctcccgcagc tttctgagcg gatcgtagga gggcgagaga tacagattct tggccagttg
     1081 ctgggtgatc gtgctggcgc cgcggggcgc ctgccggcgc gcgacgctgt cttcgatcga
     1141 cttgcggatc tcggcgacgt cgatcccttc gtggtcccag aaggcgccgt cttcggcgac
     1201 caggacggct cgcctcaggt tgggtgagat gcgcgagtac ggcacccatt gatggacccg
     1261 gcgtatctta cgcccttgtc gggccgcttc gcgctcccga agttgcatga atgcggttga
     1321 ggtcgggttt gatcgcgcga gaggccggac gtccggcaga gtcaggtaga cgtaacaggt
     1381 cactccgaat cccacaccgg ccagcgcgat gagcgcgcgg cgcacccgag gtgggacttt
     1441 cctcacggag ccacgagcgt cgaccgcagg gcggccaacg ccgccgcggc tcccgcgccc
     1501 tctggaacgt tgtagccgtg cgacgacagg gcgccttcgc acgcggccag gaactgcagc
     1561 acggtttgtg gagtggagct cgcgcccatc aggccgacgc gccagatctt gcccgcgagc
     1621 ggacccaggc ccgcgcccac ctcgatgttg tacgtcgtga gcatcgtccg ccgcacggcc
     1681 ccttcgtcca caccggccgg caaccggacc gcgttgagcg tccagaggcg ctcgctctcg
     1741 ggtggaagca tcgagagccc catggcctcg atgccggcca cgagcgcgcg atggtggcgc
     1801 tcgtgccgcg cctgccgtgc ctcgagtcct tcctcttcca ccatgagcag cgcttcgcgc
     1861 agcgcgtaga tcaacgtgct cgacatcgtg tggtggtact tgcgcccgac ccagtagtcc
     1921 tcgaggagct tcagatccag atagaagctg cggcacggga cgagccgctc gcgcgcgggc
     1981 cccgagacgg ccagcggcgc aagccccgac ggggcgccga tgcatttctg cgtgcacgaa
     2041 taggcgatgt ccacgcccca gccggcgaga tcgacttcgt gaccgccgag cgacgtcacc
     2101 atgtcggcga tgatgagcgc gccgtgttcg cgcgcgaccg aggcaagctc cctgaccggg
     2161 ttccgcactc cggttgacgt ctcggcgtgc acgatcccga cgacgtcggc gccttcgagc
     2221 ctcaactcgt cgcgaagccg ctgcggatcg acggcccttc cccactcgac gtcgatccgc
     2281 cggaccgccg cgccgtatcg ttggaacatc tgcgcgaggc gatcgccgaa atacccggtg
     2341 acgaccacca cgccgcgcgt gccgtcgcgg actgcgttgg ccaccgccgc ctccatggcg
     2401 gacgtgcccg tgccggaggt ggcgagcgtc agcgcacggc cgtcggcgcg gaacacgcgc
     2461 tgcagcgacg cgcggatctc gtccagcatc gggacgaact cgggatcgag gtggctcagc
     2521 acgggcgccg ccatcgcccg catcacgcgc ggggcgatga ggctgggtcc ggggccgagc
     2581 agaatacgtt cagtcgaggg aagcggtatg ggcatcagtg ttgtgtcggt gaatcgatct
     2641 cgtagagtct cgtagagcgc gggcttcagc ccgcgcgtgc ggcaacacgc cgagcgcgtc
     2701 gagctgatct cgaagggtgt cgatgacgga ggtcggcacg gggcgaagcg gcggccgcgg
     2761 cggtcccgcc tcgtagccaa tcaggtggag cgccgccttg aggccgggaa cgccgtaggt
     2821 gccgccgacc gatcgcgcca gcggcagcag ccggcgctgg aggagccgcg cctccacgag
     2881 acggttgtcg tgcacgagcg tcatcatctc gttgcactcg tctggcacga gcgaggcgag
     2941 cgcgaggatc gccccgtcgc accccgcgca cagcgcgtga aagagcgtgg cggccgatcc
     3001 ggcaagcacg gtgaagtcgt ccggcgtgcg cgacacgaac tcggcgatct gcccgatgtc
     3061 gctccccgat tccttgatgc cgacgatgtt cggatgaacg gcgaggcgct cgaccgcgtc
     3121 tggcagcagg ttcacgccgg tgtacatcga cacgttgtac agcagcaccg gcaccggcga
     3181 ggcctcggcg acatccgtat agaaccggac gaacgcttcc gtcgtcatct gcggcttgaa
     3241 gaacgacggc gtgcggacca gcaccgcgtc cgcgccggcg gcggccgcgc gtttgctggc
     3301 ggcgatggtg gccttcgtgg actcgcggcc cgtgccggca atcagcggac gatcggaggg
     3361 cacccccgca cggacgatgt ccacgatgcg gtcggcctcg tcgtcttcga gctgcggcgc
     3421 ctcggcgttg gacccgagca ccacgaggcc ccgcagcgac gtcttcatcc atcgcgcggt
     3481 gttgctccgc agcccggcct cgtcgagcgt gtcgtcggag cgaaacggcg tcgcgatcgg
     3541 cgtgaagatc cccgggaagc gcttcatcgc tctaattgta cagggacgcg gcctcccccc
     3601 tgcgcgagca ggttgacgcc gagcacgacg atgaaaatcg ccgccgccgg ggccagcgtc
     3661 cacggcgcgt cgccgagcag ggccacgttc gaggcgtcct gaagcatcgt tccccacgtg
     3721 ggcgtcgtgc tcgcaaagcc gagcccgatg tacgagagcg tggcttcggc gaggatgaag
     3781 gccggaagca gcaacgtccc ctgcgccttc aagtaccccc acgacgccgg gagcagatgc
     3841 cggatcagca cgcggagatc ggaggcaccg gcggcgcggg cggccgaggc gtagtctcgc
     3901 gagcgctcgg cggcgacgat cgcgcgcacg ccgcgggcca cgaccggcca gccgagcgcg
     3961 gcgaagatga ggagcagaag gaagaagacc gctccgtccg gcagcacgag cggcagcgcg
     4021 gcccgaagcg cgagcgccac gtagatcgcc ggcagcacga ggacgaactc cgtcacccgc
     4081 gagaggatcg cgtccgccca gccgccggcg tagccggcga gtcctccaat ggccgcgccg
     4141 gcgagggtgg caagcagcgt ggccagcacc gagagcgcga gcgtgaggcg ggcgccgtgc
     4201 agcagtcgcg agaacatgtc gcggccgtag ccgtccgccc cgagcaggag cagcggcgcg
     4261 ccgtcatcgg ggcggcccgt caccagcgtg ccgtcggcga accatcgcag cgtcgcgcgc
     4321 tcggagacga ccggctcgaa ccggcgctcc agacggctga ccacacgcca gggatggatg
     4381 aaaggcgccc gcagaccctc gtcgaggacg cggatgcggg tcggcggcgc gtacaggaga
     4441 tcgtcgaatc gccgatcggg ctgattcggc gccagccatg gtgcgcctaa tgcggcgatg
     4501 gccacgagac cgagcagcag ccaagccacc tgcttcacgg tcgactccct ggccgcggca
     4561 ccttcgcacc ctcgcactct cgcaccgtcg cacccttact catgccgcac ccggggatcg
     4621 gctgccgcga gcagcacgtc gcccgccgcc gttccaagcg caagaaagcc ggcccccgcg
     4681 gcggcgcacg ccgcgaccag gtagatgtcg cgggcccgga gggcgtcgaa catgaggcgg
     4741 ccgaggccgg gccacgtcgt gacgtattcc acaatgaacg agcccgagag cagggcgcca
     4801 atcgccaggc cgtagacggc gctgaccgat cgcagggaga ccggccacgc atgccgcagg
     4861 atgacgaggc gccgcggcgc gccgcgcgcc acggccgcga gcacgaacgg ctggcgtacc
     4921 gcctcgccca tcgcctgcga ctgcagccgc tcgaaggtgg cggcaatcgg cagcgacagc
     4981 gcgagcaccg gcagcggcag gtgccacagc acgtcggcga tccaggccgg ccacgacgcg
     5041 gcagcggcgc cggacgaggt catgctgccg gagggaagcc atcccgttcg cgccgcgacg
     5101 aacacgaaga gcagcgacgt gacgagggga ggcatcgaca gcacgacgag cgacgcgccg
     5161 cggacgatcg acgccggcac gccttctccc cgcgacccgg tgaagacgcc cagcgagatc
     5221 ccgatgagcg tggcggccac gagagacacg atgcccagca gcgccgtgtt cccggcagcc
     5281 ttcgcgacga gcggcccgac cggccggccg tagagaaagg agtcgccgaa gtcgaagcgc
     5341 acggcgcgtc gcacccacag ccaccactgc gcggcggggc ttcgatcgag atcgaaccgc
     5401 gcccgcgtgc tctcgacgtc cacccgcgac gcaaacggac cgagctgcgc cgtcatgtcc
     5461 cccggcgcga ggcgggcgag caggagcgcc accgaggacg cgacggcgac gaggacgatg
     5521 gcgaagagca gccgtcgaag gaagaagcgc gccatttact ccacagctcg cgtgtgttgg
     5581 cgggctccgg cgaaggccac ggcgacgaga cggcctgttg tgctaccgac gagcgttggc
     5641 aacatcaccg acaccagcga gttcccgggt tagtgcccac ccgccacgct gagcgagtcg
     5701 gcattccaga gtacgggagg gcgcgtgatg gacgggacca tgccgcgcac gcgccggctg
     5761 tgcgcgtagt agaggcgcgg cgccgcgaag tacagcaccg gcgcctcacg ggccagcgtg
     5821 gcctgcacct cgttgaacag ttcatgacgc cgggcatcgt cgagcgtggc ggcctgttcg
     5881 tgcatgagcg cgtcgatgga tttctcccag tcggtagccg gcgccttctg ctgcatgttc
     5941 cagaggtggg cccctcccga gctgagccag taatcgaggt tgcccgcggg atcgagctca
     6001 gtggcgagcg gccgcatgta gatcgcgtca tagtcgcagg ccagcagccg cttgatcatc
     6061 gccgagaact ccatcggcac gatgtcgagc gcgatgccgg cggccgccac ctgctcgcgc
     6121 agcaccgtag tgccgcgctc gtagtagccg atgccctgct gcgtgatgac ggcaaagcgc
     6181 gcctcggcgc cggaggcgtc ttcgacgacg ccgttgccgt tgcgatcctc gaggccgata
     6241 ctcgcgagca gctgccgcgc gcgtgccaga tcgtgcgggt agcgcgggac gttgggcgag
     6301 aaccacacct tgttgccggg cgtaatgggg ccccacacgg ggaccgcctc gcccaggaac
     6361 accgtgcgcg cgaacgcttc gcgatcgacg gcgtgcgaga tcgcctggcg gaattcgcgc
     6421 ttctggacga aggcaaagcg acggtccttc ttcttgacct ccggcttgag acagaaccag
     6481 aaggcgtcgg cgtcagccgt gacgccgagc tcgatcatcg tgagccggcc ttcctgctcg
     6541 gcgcgacgga ccggaatgta gtcgtcggac cgcagctcgc tgtgcgtgag gtcggaggcg
     6601 cccgactgga gccgcagcag ctcggcgttc tggtcgggca cgatctcgat gacgagccga
     6661 tcgaggtacg gcagctgatc gccgttgggc gccctgcgcc agtaccgggg attgcgatcg
     6721 aacaccagcc gctggcccgg ctggtactcc cggagcagga acgggcccgt gccgacgatg
     6781 tcggaggggg gcgtggccgt gctccacgcc ctggccagcg tcccgtcctg aagcgccggc
     6841 tcgagtgcgt gccgcggcag gattggcagc atgtcgagca ggcgcagccc cgggcccgcc
     6901 ggcgacgcga agccgatgac gacagtggct gcgtcgggcg ccgaggccgt gatgggcctg
     6961 ccgcccacgg tcaggctgct ggccacgacg ctcttgaccc tcgggtcgta caccgcctgg
     7021 atcgagaaga ggacgtcggc cgaggtgaac ggcgtgccgt cggaccagac gactcccggg
     7081 cgcaggtgca gggtgtgcgt ccgcccgtcg ctcgaggatt cccaccgctc cgccagccag
     7141 ggctccagct cgaaggttga ccggttgatg cggaccaggc gtccgtgcat cagcgtggag
     7201 atggtgtccg aggt
//
LOCUS       DBNGSH010000778         2590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1274437, whole genome shotgun sequence.
ACCESSION   DBNGSH010000778 DBNGSH010000000
VERSION     DBNGSH010000778.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1274437"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="AC16CY_17880"
     CDS             <1..471
                     /locus_tag="AC16CY_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3355726.1"
                     /translation="DDDWSDGFGLKFFGAMRLSRAAWPHLRSSAGAIVNIVGIGGRTG
                     SAEFAIGGAVNAALLNLTKVLADRGVRDGVRVNAINPGGIATDRLQTRLKAFAAERNV
                     DLPAAEREMARALGVARFGQPDEIARLVAFLASPQSAFCQGSIVDADGGQTRTL"
     gene            692..1681
                     /locus_tag="AC16CY_17885"
     CDS             692..1681
                     /locus_tag="AC16CY_17885"
                     /inference="COORDINATES: protein motif:HMM:NF017773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3355727.1"
                     /translation="MPTLWMITNRQQRGGELGKDAGPLTFWRTDKTGTVGKLKKLNQW
                     EELTEAKFKGELRRSVDEEFPDLPDDENEHEHHVTLFVHGFNNAWEDALRRYMQISSA
                     ILNKPKLGTCVLFTWPSDGQKVGYYPDRKDARESADELAEVLALLYDYLAERQEAAMR
                     SGLKTCKAKTSIIAHSMGAYVVQKAMQTVWTRKNQPLLMSLVNQLLLVGADVDNDLFG
                     SGETVDKGDGDAIANLTYRVTALYTGLDSTLGVSAGLKHFGKRRLGRSGLDDRGAVPD
                     NVWDADCTEIFRKAKAENTHSAYFNVPRTQELLVAILRGVDRLVLKTQFPELG"
     gene            complement(1745..>2590)
                     /locus_tag="AC16CY_17890"
     CDS             complement(1745..>2590)
                     /locus_tag="AC16CY_17890"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3355728.1"
                     /translation="AVDPKLNRLYVTTGNNYSDPTTTMSDAFLALDLDSGKILWSRQM
                     TEKDAYTAACRLPDKTNCAESNGPDFDFGASPILVTLASGRRMLVAGQKSGIVHALDP
                     DKGGEILWQTRVGRGGTMGGVQWGSAADASNVYVALSDIGRIMLTYSNSTDADPKQGG
                     GMFALRLEDGERVWYTPPPGCGTRTRCSPAQSAAVSALPGVAFSGSVDGHMRAYSAAD
                     GTIIWDFDTIRAYETVNGVPGRGGSIDGPGPAIGGGMVFVNSGYPTAGGTPGTVRRAF
                     SGYGK"
BASE COUNT          463 a          820 c          868 g          439 t
ORIGIN      
        1 gatgacgact ggtccgacgg gttcggcctg aagttcttcg gcgcgatgcg cctgagccgc
       61 gcggcctggc cgcacctgcg ctcgtcggcc ggcgccatcg tcaacatcgt ggggatcggc
      121 ggccggaccg gctcggccga gttcgcgatc ggcggcgcgg tgaacgcggc gctgctgaac
      181 ctcacgaagg tcctggcgga tcgaggcgtc agagacggcg tgcgcgtcaa cgcgatcaac
      241 cctggaggca tcgcgaccga ccggttgcag acgcgcctga aggcgttcgc ggccgagcgc
      301 aacgtggacc tacccgccgc cgaacgggag atggctcgcg cgctcggcgt cgcgcgcttc
      361 ggtcagccgg acgagatcgc gcggctggtg gcgtttctcg cctcgccgca gtcggccttc
      421 tgccaggggt cgatcgtgga cgccgacgga gggcagacga ggacactctg aaccgggaac
      481 ttgtgttgag aatgagctct gtcgttcacg cttctcgctt ctctgttcgc agttcgtgtt
      541 caatttcggg gttcggttcg aggtttccgg ttcggcgttc tatatttccg gtttgctcga
      601 ggccgcgaac cccgaacagt aaactcagaa cacgcacctg aaactgaaca cgaaccgaga
      661 agcgagaagt gtgaactggg ggaagacagc tatgcctact ttgtggatga tcacgaatcg
      721 ccagcagcgc gggggagagc tgggaaagga cgccggccct ctgacgttct ggcggacaga
      781 caagacaggc accgtcggca agctcaagaa gctcaaccag tgggaggagc tcacggaggc
      841 gaaattcaaa ggcgagctca gacgatccgt ggacgaggag tttcccgatc tgcccgatga
      901 cgagaacgag cacgagcacc acgtgacgct gttcgtgcac ggcttcaaca acgcgtggga
      961 ggacgcgctc agacgctata tgcagattag cagcgcgatc ctgaacaagc cgaaactggg
     1021 cacgtgcgtg ctctttacct ggccgtccga cggccagaaa gtcgggtact accccgaccg
     1081 caaggacgct cgcgaatcgg ccgacgagct cgcagaggtg ctggcgctcc tgtacgacta
     1141 cctggccgag cgccaggagg cggccatgcg cagcgggctc aagacgtgca aggccaagac
     1201 gtcgatcatc gcccacagca tgggcgccta cgtcgtgcag aaagcgatgc agaccgtctg
     1261 gacgcggaag aaccagccgc tcctcatgag tctcgtcaat caactccttc tggtaggcgc
     1321 cgacgtggac aacgatctgt tcggctctgg tgagacggtc gacaagggcg acggagatgc
     1381 gatcgccaac ctcacgtatc gcgtcaccgc gctctacacg gggctcgact cgacgctggg
     1441 tgtctcggcc ggcttgaagc acttcggcaa gcggaggctg gggcgatccg ggctggacga
     1501 ccgcggcgcc gtcccggaca acgtctggga cgccgactgt acggagatct tccgcaaggc
     1561 gaaggcggag aacacccact cggcgtactt caacgtcccg cggacgcagg agctcctcgt
     1621 cgcgattctt cgaggcgtcg atcggttagt gctgaagacg cagtttcctg aactcgggtg
     1681 aaggggtcgg gagtcatttt cccgggagga tgcagcggga aaatgacacc cgacccccat
     1741 ttttctattt accgtacccc gaaaaggcgc ggcggacggt gcccggtgtg ccgccggcgg
     1801 tggggtagcc cgagttgacg aagaccatcc cgcccccgat cgccgggccg ggtccgtcaa
     1861 tcgatccgcc gcggcccggg acgccgttca cggtctcgta ggctcgaatc gtgtcgaagt
     1921 cccagatgat cgtgccgtcg gccgccgaat acgcgcgcat gtggccgtca accgagcccg
     1981 agaaggccac tccgggaagg gcgctcacgg ccgccgactg cgccggacta caccgcgtgc
     2041 gcgtgccgca gcccggcggc ggcgtgtacc acacgcgctc gccgtcctcg agccgaaggg
     2101 cgaacatgcc gccgccctgt ttcggatcgg cgtcggtgct gttgctgtag gtcagcatga
     2161 tgcgcccgat gtcggagagc gccacgtaga cgttgctcgc atccgccgcc gagccccact
     2221 gcacgccacc catcgtgccg ccgcgcccga cgcgtgtctg ccacaggatt tcgccgcctt
     2281 tgtcgggatc cagcgcatga acgatgcccg atttctggcc cgcgacgagc atgcggcgtc
     2341 cgctggcgag cgtgacgagg atgggcgagg cgccgaagtc gaagtccggt ccgttcgact
     2401 cggcgcagtt ggtcttgtcg ggcaggcggc atgccgccgt gtacgcgtcc ttctcggtca
     2461 tctgccgcga ccagaggatc ttcccgctgt cgagatccag cgcgaggaac gcgtcgctca
     2521 tcgtcgtcgt cgggtcgctg tagttgtttc ccgtcgtgac gtagagccgg ttcaacttcg
     2581 gatcgaccgc
//
LOCUS       DBNGSH010000779         2820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1444626, whole genome shotgun sequence.
ACCESSION   DBNGSH010000779 DBNGSH010000000
VERSION     DBNGSH010000779.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1444626"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="AC16CY_17895"
     CDS             complement(<1..631)
                     /locus_tag="AC16CY_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3355729.1"
                     /translation="MTDLIRRRSSVILAVIAAAAVSACSCSVTGPSGGGAQISGRVTN
                     IAAAGSRSSVSALTALTTSTPTVASNGSGTLKVTITGTNIESEIDGSGRFTLDGVPPG
                     TVELTFSGDGVSATLRISGIEEGDRIEIEIRLDGNRVTVDSERRDRDDDDDEGDDDDN
                     DDDDNDDDDNDEFEARGTVSGLTGTCPALTFTLGERTVTTSSTTAFKDGC"
     gene            complement(808..1401)
                     /locus_tag="AC16CY_17900"
     CDS             complement(808..1401)
                     /locus_tag="AC16CY_17900"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIR3355730.1"
                     /translation="MIASRTRSAVATCALTLLLSLASASPAPAQSNFVSPFIGFDFGG
                     DSGCPAISGCEDKNVNWGVGVGSLGRVFGAEFEFAYIPSFFGEIPGLSSSVLTLMGNV
                     MLAPKFGPVQPYGLAGLGLIKTRTELTLPAFLESSNNHFGWDVGGGLIIYPSQRFGIR
                     GDIRYFHAFQDLEVLGITLGDTKLDFGRASAGVVFKF"
     gene            complement(1498..>2820)
                     /locus_tag="AC16CY_17905"
     CDS             complement(1498..>2820)
                     /locus_tag="AC16CY_17905"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3355731.1"
                     /translation="RDLLKTGLAAAGGLAITQQGVLAQSQSATTSTGQRFRGWVSRGA
                     GPGRTTLHDLTLRPIGGRQVVVRTEATNLCYSNVGAVLGLQPAGFGRLNQMALIQGHG
                     GVGVVEAVGPDVRRVRVGDRVCVSGTPQCGACYQCLRGRADMCQFLGRQGPDDLVPIA
                     DMSDGTPVYANSHIGGLAEKMVTFEEWVVPVFTKAPAAELGMVCSCVAVAGLGATTSQ
                     NVAIVEPGSIVAVVGCGPLGLSAVQGARISGASTIIAIDPIRVRRELALKVGATHVLD
                     PNVEGARLVERVREIASGPSNRLWSGGRDSGGLLGGAGADFVVEAVGADAAPPKLEAA
                     PDPTGIVPLRQAYEMTAAGGHIVTTSLPRGTISLPAAFFTIGGRTHHAGQAGGCNPMR
                     DIPRFVELLDKGQYNARALATTVVPIEGMREAYEEVAYRSTVTAIMTP"
BASE COUNT          481 a          942 c          933 g          464 t
ORIGIN      
        1 agcagccatc cttgaacgcc gtggtgctgc tggtcgtcac agtgcgctcg ccgagcgtga
       61 acgtcagcgc cggacaggtg cccgtcaggc cagacaccgt gccgcgcgcc tcgaattcgt
      121 cgttgtcatc gtcatcgttg tcgtcgtcat cgttgtcgtc gtcatcaccc tcgtcgtcat
      181 cgtcgtcgcg gtcgcgtcgc tccgagtcga ccgtgacgcg gttgccgtcg agccggattt
      241 cgatctcgat ccgatcgcct tcctcgatgc cgctgatccg gagcgtggcg ctcacgccgt
      301 cgccactgaa ggtcagctct accgttcccg ggggaacgcc gtccagcgtg aaccggcccg
      361 agccgtcgat ctcggattcg atgttcgtcc cggtgatggt gaccttgagc gtgcccgatc
      421 cgttggaggc caccgtggga gtgctggtgg tcagggctgt caatgcactg accgacgagc
      481 gcgagccggc cgcggcgatg ttggtgacac ggcctgaaat ctgggcccct ccgccggagg
      541 gacctgtcac ggagcagctg cacgccgaga cagcggctgc ggcgatgacg gcgaggatca
      601 cggacgaccg acgtcgaatc aagtcagtca ctgggcgttc tccggggggt cgccgtccgg
      661 aacgcaagcc acgttccgtc ctcgtcccgg gcggcaacct cacttcagcc agcgacttac
      721 gaattcaagg tcgaaaccgg atccgtccgg agagcgacca cttcgttgta agacttacac
      781 ggacggctcg gccgaccgac actcagatca gaacttgaaa acaaccccgg cgctcgcccg
      841 tccgaagtcg agcttggtgt cgcccagagt gattccgagg acctcgaggt cctggaaggc
      901 atggaagtag cgtatgtcgc cacggatccc gaagcgctgg ctcgggtaga tgatcaggcc
      961 gccgccaaca tcccacccga aatggttgtt gctgctttcc aggaaggccg gcaacgtgag
     1021 ctctgtgcgt gtcttgatga gcccgagacc cgcaagtccg tacggctgca cgggtccgaa
     1081 cttgggggcg agcatcacgt tgcccatgag ggtcagaacg ctcgacgaca ggcccgggat
     1141 ctcgccgaag aaagagggga tatacgcgaa ttcgaattcg gcgccgaaga cgcggccgag
     1201 cgacccgaca ccgacgcccc aattgacgtt cttgtcttcg cagccggaga tcgctgggca
     1261 accggaatcg ccgccgaagt cgaacccgat gaacgggctg acgaagttac tctgggcggg
     1321 agcagggctg gcactggcca acgacaggag cagcgtcagc gcgcaggttg caacggccga
     1381 tctcgtccgt gaagcgatca tgtcaacacc tccgccggcg agttctgcaa gaaccggccc
     1441 ttccagaaac atgggaaaaa ggcgcagaag tgcgcagcct ccgggggtac cgtgtggcta
     1501 aggggtcatg attgccgtca cggtcgagcg atacgcgacc tcttcatatg cctcgcgcat
     1561 gccttcgatt ggcaccacgg tcgtggccag agctctcgcg ttgtactgac ctttgtcgag
     1621 caactcgacg aagcgcggga tgtcgcgcat ggggttgcag ccgccggcct ggcccgcgtg
     1681 atgcgttcgc ccgccgatcg tgaaaaacgc ggcaggcagc gagatcgttc cccgtgggag
     1741 gctcgtcgtc acgatgtggc caccggctgc cgtcatctcg tacgcctggc gcagcggcac
     1801 gatgcccgtc gggtcggggg cggcctcgag cttcggcggc gcggcatcag cgcccaccgc
     1861 ctcgacgacg aagtccgcgc cggcgcctcc aaggagtccg ccagaatcgc gcccgccgga
     1921 ccacagacga ttgctcgggc cgcttgcgat ctcgcgcact ctctcgacga ggcgtgcgcc
     1981 ctcgacgttg ggatcgagca cgtgcgtggc ccccactttc agcgcgagct ctcggcgcac
     2041 gcggatcgga tcgatcgcga tgatcgttga cgcgcccgag atgcgtgccc cctgaacggc
     2101 gctcagcccg agcggtccgc agccgacgac ggcgacgatc gatcccggct cgacgattgc
     2161 gacgttctgc gacgtcgtcg cgccgaggcc ggccacggcc acgcagctgc agaccatgcc
     2221 gagctcggct gccggcgctt tcgtgaagac gggcacgacc cattcctcga aggtcaccat
     2281 cttctcggcg aggccgccga tgtgcgagtt ggcgtagacc ggcgtgccgt cgctcatgtc
     2341 cgcgatcgga accaggtcgt cggggccctg cctgccgagg aactgacaca tgtcggcgcg
     2401 gccccggagg cactggtagc aggcgccgca ctgcggagtt ccggacacgc acacgcggtc
     2461 gcccacgcgc acgcggcgaa cgtccgggcc gacggcttcg acgacgccca caccgccatg
     2521 gccctggatg agcgccatct gattgagccg tccgaatccg gcgggctgca ggcccagcac
     2581 ggcgcccacg ttcgagtagc agagattggt ggcttcggtg cgaacgacga cctgacgtcc
     2641 gccgatcggc cgcagcgtca ggtcgtgaag cgtcgttcgt ccggggccgg cgccgcgcga
     2701 tacccagccg cgaaaccgct ggccggtgga agtcgtggcg ctctgcgact gcgcgagcac
     2761 gccctgctgc gtgatggcca atccgccggc cgccgccagg ccggtcttca gcagatcacg
//
LOCUS       DBNGSH010000780         3155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3991721, whole genome shotgun sequence.
ACCESSION   DBNGSH010000780 DBNGSH010000000
VERSION     DBNGSH010000780.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3991721"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..437
                     /locus_tag="AC16CY_17910"
     CDS             120..437
                     /locus_tag="AC16CY_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR3355732.1"
                     /translation="MNTDMDTRPHRDYGFVIGLVAGTCVGAALAMWLAPRAASELRER
                     MTDSARSLGQRASEGFQEANTLVAEAVGELGRRGRGVRDEVAGAVARGAREVERRATA
                     SLA"
     gene            577..1263
                     /locus_tag="AC16CY_17915"
     CDS             577..1263
                     /locus_tag="AC16CY_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3355733.1"
                     /translation="MRNVYALAVLGLTVALAPACATKKHVRTEVGSVNAKVDTLAGSL
                     EQTQERTRQNESRIAAVDVKAEAAGRSAAEAGTAATAAATNAASATREVDGRLTGRIN
                     QAEASARRLIYEVALTEDEGNFTFGDAALPDEAKARLDEMVAQLKADPKGVFIEVEGH
                     TDNVGKSEYNEQLGLERAETVKRYLYMQHQVPLHKINVVSYGEEKPVAPNNTRTGRAQ
                     NRRVVVRVLS"
     gene            1392..1985
                     /locus_tag="AC16CY_17920"
     CDS             1392..1985
                     /locus_tag="AC16CY_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HIR3355734.1"
                     /translation="MSGKNTAVFGIYPTHESADAAVDALRAKGFRNTDVSVLFPQNSG
                     SKEFGHQKGTKSAEGAVAGGGTGAVIGGALGWLAGIGMLAIPGVGPFIAAGPIVAALA
                     GMGAGGAIGGTTGALIGLGIPEYQAKRYEGRMRKGGILLSVHADDQEWVRKGRQILER
                     TGAEDISSTGETKGDFGNADRPTSRPEQVAPVAVVKS"
     gene            2134..2859
                     /locus_tag="AC16CY_17925"
     CDS             2134..2859
                     /locus_tag="AC16CY_17925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3355735.1"
                     /translation="MVSIVLADDHAIVRQGLRTLLESDPLLEVVGEASDGRDAIELVK
                     RRKPDILIADLMMPGVNGLQLTTSVSRLKLGTRVIVLSMYRDDAYVLEAFRNGAAGYV
                     AKESGGAELFQAIRETLAGRSYLSPAISQASEGSYAFQGSALRTPRSGSTGRHATLTA
                     REQSVLRLVLEGAGSRDIADRLKLGSRSVEGHRTALMQKLGLKTRDELTRYALQRGVA
                     LGKKARSAVKKPPRRLAAAETTR"
     gene            2907..>3155
                     /locus_tag="AC16CY_17930"
     CDS             2907..>3155
                     /locus_tag="AC16CY_17930"
                     /inference="COORDINATES: protein motif:HMM:NF018455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HIR3355736.1"
                     /translation="MTGLDEVLRGGLPAGRMTLLSGGAGSGKSLIALQCALHGARAGE
                     PCIVVLFEERAAAVRQNVLSLGWDVRSLERNKKLFFLDA"
BASE COUNT          581 a          991 c         1110 g          473 t
ORIGIN      
        1 ctccatctct cccctggctg tcggcgtcgg agtgcgcgag atcatcgcct cgctgctgtg
       61 cctttgtgaa catccgcccc cgcgccgcca gcgtaaggta cccccaggag caacacggca
      121 tgaacacaga catggacact cgcccgcatc gtgactacgg cttcgtgatc ggactggtgg
      181 ccggcacgtg tgtcggcgcc gccctggcga tgtggctcgc cccacgggca gcttcagagc
      241 ttcgtgagcg catgaccgat tcggcgagaa gcctcggcca gcgggcatcc gagggatttc
      301 aagaggccaa cactctcgtc gccgaagcgg ttggcgagct gggccgcaga ggtcggggcg
      361 tccgggacga ggtggccggg gcagtggcac gcggcgcacg cgaggtcgag cgccgtgcca
      421 cagcctctct ggcgtagtcg cgccaggccc gctcgtcgcc actgggagat cgtgaccggg
      481 tccaccaagg ggtggtgacg gccgcgttcc cgaacatgct agggctgtac tcgtcgcgca
      541 cagccacgga acaacgtcga tccaaaggag gcagtcatga ggaacgtgta tgcactcgcg
      601 gtgctcggcc tgacggtcgc actcgcaccg gcctgcgcga cgaagaagca cgtgcgcacc
      661 gaggttggca gcgtcaatgc gaaggtggac accctcgcgg gctcgctcga acagacgcag
      721 gagcggacgc gccagaatga gagtcggata gccgcggtgg acgtcaaggc cgaagcggcc
      781 gggcgctcgg ctgcggaagc tggaacggcg gcgaccgccg cggccacgaa cgcggccagc
      841 gcgacccggg aagtcgatgg ccgcctgacc ggtcggatca accaggcgga ggccagcgcc
      901 cgtcgactca tctacgaggt ggctctcact gaagacgagg gcaacttcac gttcggcgac
      961 gccgcgctgc cggacgaagc caaagcccgc ctcgacgaga tggtcgcgca gctgaaggcg
     1021 gacccgaagg gcgtgttcat cgaagtcgaa ggtcacaccg acaacgtcgg aaagtctgag
     1081 tacaacgagc agttgggcct cgagcgtgca gagacggtca aacggtatct ctacatgcag
     1141 caccaggtgc cgctgcacaa gatcaacgtc gtgagctacg gcgaggagaa gccggtcgcg
     1201 ccgaacaaca cgcggacggg tcgcgcgcag aaccgccgcg tcgtcgtccg agtcctgtcg
     1261 taggccttca caccggcacg gttgcgatgt cgcgcgccgg tggacacacg gccccgtcca
     1321 caatcctcgg attcgcagag acggcggctc cgcccgaatc aggcgcgccg tcagcaacac
     1381 tggaggcgag tatgtctggc aagaacacag cagtattcgg aatctacccg acccacgaga
     1441 gcgcagacgc ggccgtcgat gctctgagag ccaaggggtt tcggaacacg gacgtgtccg
     1501 tgctgtttcc gcagaactcc ggttcgaagg aattcggcca ccagaagggc acgaaatccg
     1561 ccgagggcgc cgtggccggc ggcggaaccg gcgcggtcat cggcggcgcg ttgggatggc
     1621 tggccggcat cggcatgctg gcgatccccg gcgtcgggcc gttcatcgcg gcgggtccca
     1681 tcgtggcggc gctcgcaggc atgggcgccg gtggagccat cggcggcacg accggcgcgc
     1741 tcatcggcct gggaattccg gagtatcagg cgaagcggta cgaggggcgg atgcggaagg
     1801 gcgggattct gctgtcggtg cacgccgacg atcaggagtg ggtgcgcaag ggcaggcaga
     1861 ttctcgagcg caccggcgcc gaagacatct cctcgaccgg tgagaccaag ggcgacttcg
     1921 gcaacgcgga ccgcccgacc agccgacccg agcaggttgc cccggtcgcg gtcgtcaagt
     1981 cctgaggcgc tcccacacga gcacgggcag acgcatcgtg tcgggaagcg ccctgttcaa
     2041 tacaggacag acccctcggg ttcggacgcc tatcctgtgg ctctaaacgc ataggcggag
     2101 ctgtggatca ccgccggtcc gaaggagaac gtcatggtga gcatcgtact ggcggatgac
     2161 cacgcgatcg tgcggcaggg actgcgcacg ttactggaat cggatccgct gctcgaggtt
     2221 gtgggcgaag cctcggatgg acgcgacgcg atcgaactgg tcaagcggcg gaagccggac
     2281 atactcatcg ccgatctgat gatgccgggg gtgaacggcc tgcagctcac gaccagtgtc
     2341 tcccgcctga agcttggcac ccgcgtcatc gtgctgtcca tgtaccggga cgatgcgtat
     2401 gtactcgaag ccttcagaaa cggcgcggct ggctatgtcg ccaaagagtc cggcggggcc
     2461 gagctcttcc aggccatccg ggaaaccctg gctggtcgtt cctacctgag ccctgcgatc
     2521 tctcaggcct ccgaaggctc ctatgcattc cagggatcgg cgctgcgcac ccctcggtcc
     2581 ggttcgactg gccggcacgc gacgttgacc gcccgtgagc agtcggtcct gcgcctggtc
     2641 ctcgagggag cgggtagtcg tgacatcgcg gaccgtctga agctcggttc acgcagcgtc
     2701 gaaggccatc gcaccgcctt gatgcagaag ctggggttga agacccgcga cgaactgact
     2761 cgctatgccc tgcagcgtgg ggtcgcactt gggaagaagg caaggtccgc tgtgaagaaa
     2821 ccgccgcggc gactcgcagc ggccgagacg acgcgatgag agcggcggta ccacggagac
     2881 gagaactcac gaaagtccct accggtgtga ccgggctgga cgaggtgttg cgcggcgggc
     2941 tgccggccgg gcgcatgacg ctgctcagcg gcggtgccgg gagtggcaag agcctcatcg
     3001 ccctgcaatg cgcgctgcac ggcgccaggg cgggcgagcc gtgcatcgtg gtgctgttcg
     3061 aggaacgcgc cgccgcggtt cgtcaaaacg tgttgagcct gggatgggac gtgcggtcgt
     3121 tggagcggaa caagaaactc ttcttcctgg atgcg
//
LOCUS       DBNGSH010000781         2791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2972474, whole genome shotgun sequence.
ACCESSION   DBNGSH010000781 DBNGSH010000000
VERSION     DBNGSH010000781.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2972474"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="AC16CY_17935"
     CDS             <1..414
                     /locus_tag="AC16CY_17935"
                     /inference="COORDINATES: protein motif:HMM:NF027286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HIR3355737.1"
                     /translation="YESATLLIRLYELRREPTMREARNWFAREFNPASIDEVMQALNG
                     PHSGHFRMASSYWDMAASFVLNGAIDEKMFNDANGEQLVVFAKLEPFLAEYRKRTGNP
                     AYLSSLETIVLKRPGAKESLPGIRERFRAMASASR"
     gene            complement(420..>2791)
                     /locus_tag="AC16CY_17940"
     CDS             complement(420..>2791)
                     /locus_tag="AC16CY_17940"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3355738.1"
                     /translation="AGAGAATRLSAQSQGTLTGVVVDALGARVPGAAVTLLRDGQSVQ
                     ETRSAADGAFSFSGLAPDRYQVRVSAQGFQPNTSEPVFVGGEGRAPLEIALSIGPLRQ
                     EVLVTAAATGQLESRTGAPVTIIDSPTLEAINKLDLLEALRLVPGSQVVQQGQRGSVT
                     SFFLRGGNSNFTKVLVDGVPSNDIGGAFDFSQLAMAGVDRIEVLRQTNSVIYGSDALT
                     GVVDISTPRGVSRVPLIEYDVDGGNLSTINSALSIGGAVKRFDYYSQYSYYTTDNSTP
                     NSEYRNGTYAGRFGVALGSSTNLYGTLRHLDGRHGNANGLDLFGIPDDSISDSGQTYA
                     SVAVDSQVSQRWQATVRFGSVDQRSTFTNPAPTGAPFDSFGTINYLGDTVTLEGADGR
                     SVTGQAILDYGFGTFPSVFESRTTRGALFGQTTFQATPAFAVSAGGRFEHEAGYGDPD
                     EDPTTTRNNGGAFVEGRAAFANRAYITAGIGVEHNEVFDTAYTPRLSAAVYVRDPSNG
                     TVGETKVAFNVGTGIKAPSVFQAQNALSELVPPGSGVTPLGAERSRGLDVGLEQVFAS
                     GRLRARVGYFNNAFTDLIEFVGPGGLIQAGVPPEVAATTPGAYLNSSSYDAQGIELAA
                     DAMIGMGLRVSGSYTYLDAEVTEALGAGGAEPVFNPAFPNIPIGPYGALVGERPFRRP
                     ANSGTLFVSYTGGPVSLALSGYFAGRRDDSTFLSDGFYGYSMLLPNQDLDPAYQKIDL
                     SGAYRVHPRVRLYASIENLFDQDYAAVYGFPSLPFAIRAGATLSVGGSR"
BASE COUNT          470 a          924 c          945 g          452 t
ORIGIN      
        1 tacgagagcg ccacactgtt gatccgcctg tacgagctgc gccgcgagcc gacaatgcgc
       61 gaggcgcgca actggtttgc gcgcgaattc aatccggcca gcatcgacga ggtgatgcaa
      121 gcgctgaacg gcccgcacag cggccacttc cggatggcgt cgagctactg ggacatggcc
      181 gcctcgttcg tgctgaacgg cgccatcgac gagaagatgt tcaacgacgc caacggcgag
      241 cagctcgtgg tgttcgcgaa gctggagccg ttcctcgccg aataccgcaa gcgcaccggt
      301 aatccggcgt atctctcgag cctcgagacg atcgtgctga agaggcccgg agcaaaggag
      361 tcgctcccgg ggattcgcga gcgcttcagg gcaatggcgt cggcgtcgcg gtagaggagc
      421 taccggctac cacctacaga gagggtggcc cccgcccgaa tggcgaacgg cagcgacggg
      481 aatccgtaca cggcggcgta gtcctggtcg aacaggttct cgatgctcgc gtagaggcgc
      541 acccgcggat gaacgcgata cgcgccgctc agatcgatct tctgatacgc gggatcgagg
      601 tcctggttcg gcagcagcat cgagtacccg tagaatccgt cggacaggaa cgtgctgtcg
      661 tcgcgccggc ccgcgaagta tccggacaag gcgagcgaga ccgggccccc ggtgtagctc
      721 acgaacagcg tgcccgaatt ggccgggcgg cggaatgggc gctcgcccac gagcgcgccg
      781 tacgggccga tgggaatgtt cggaaacgcg gggttgaaca ccggttccgc gccgcccgca
      841 ccgagcgcct cggtgacctc cgcgtcgaga tacgtatagg agccgctgac gcgcagcccc
      901 atgccgatca tcgcgtcggc cgcgagctcg atgccttgcg cgtcgtacga ggacgagttg
      961 aggtaggcgc cgggtgtcgt ggcagcgacc tcgggcggca cgcccgcctg gatgagaccg
     1021 ccggggccga cgaactcgat cagatccgtg aacgcgttgt tgaagtagcc gaccctcgcc
     1081 cgcagccggc ccgacgcgaa cacctgttcg agccccacgt cgaggcctct gctccgctcc
     1141 gccccgagag gagtaacgcc cgatccaggc ggcaccagct cggagagggc gttctgcgcc
     1201 tggaagacgc ttggcgcctt gatcccggtg ccgacgttga aggcgacctt cgtctcgccg
     1261 acagtgccgt tcgacgggtc tctcacgtag accgccgccg acagcctcgg cgtgtaggcc
     1321 gtgtcgaaca cttcgttgtg ctcgacgccg atgccggcgg tgatgtaggc ccgattggcg
     1381 aatgccgcac ggccttcgac gaacgcgcct ccgttgttcc tcgtcgtcgt cggatcctcg
     1441 tcgggatccc cgtagccggc ctcgtgctcg aagcggccgc ccgccgagac ggcaaacgcc
     1501 ggagtggcct ggaacgtcgt ctggccgaag agcgcgcctc gcgtcgtgcg gctctcgaac
     1561 acggagggga acgtgccgaa gccgtagtcg aggatcgcct gtcccgtgac cgatcgcccg
     1621 tcggcgcctt cgagcgtcac cgtgtcgccg agatagttga tggtcccgaa cgagtcgaac
     1681 ggcgccccgg tcggagccgg gttcgtaaag gtgcttcgct gatcgacgga tccgaacctg
     1741 accgtagcct gccaccgttg gctcacctga gaatcgacgg ccacgctcgc gtaggtctgt
     1801 ccgctgtcgg aaatcgagtc gtcagggatg ccgaacaggt ccaggccgtt ggcgttgccg
     1861 tgccggccgt cgagatgccg cagcgtgccg tacaggttcg tgctcgatcc gagggccacg
     1921 ccgaagcggc cggcgtaggt gccgttgcga tactcgctgt tgggcgtgct gttgtcagtc
     1981 gtgtagtacg agtactgcga gtagtagtcg aaccgcttca ccgcgccgcc gatcgacaga
     2041 gcgctgttga tcgtgctcag gtttccgccg tccacgtcgt actcgatcag cggcacgcga
     2101 gacacgccgc gcggcgtgct gatgtccacc acaccggtca gcgcatcgct cccgtagatc
     2161 acgctgttgg tctggcgaag cacttcgatg cgatcgacgc ccgccatcgc aagctgcgag
     2221 aagtcgaacg cgccgccgat gtcgttggac ggcacgccgt ccacgaggac tttcgtgaag
     2281 ttggaattgc ctccgcgaag gaagaaggac gtgacgctgc cgcgctgccc ctgctgaacg
     2341 acctgcgatc cagggaccag gcgcagcgct tcgaggaggt cgagcttgtt gatggcctcg
     2401 agcgtggggc tgtcgatgat cgtcactggg gcgcccgtgc gcgattcgag ctgcccggtc
     2461 gccgcagccg tcaccaggac ttcctggcga agcggaccga tgctgagcgc gatctcgagc
     2521 ggagcacgcc cttcaccgcc gacaaacacc ggttcgctcg tgttcggctg aaaaccctgc
     2581 gcgctgaccc gcacctggta tcgatccggc gcgagaccgg aaaacgagaa ggcgccgtcg
     2641 gcggcgctcc tcgtctcctg gaccgactgc ccgtcccgaa gcagcgtgac cgccgcgccg
     2701 ggcactcgcg cgccgagcgc gtcaacgacg acgccggtca gcgtgccctg gctctgggcg
     2761 gacaggcgtg tcgccgcgcc tgccccggcc a
//
LOCUS       DBNGSH010000782        16709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1784662, whole genome shotgun sequence.
ACCESSION   DBNGSH010000782 DBNGSH010000000
VERSION     DBNGSH010000782.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 16709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16709
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1784662"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2022
                     /locus_tag="AC16CY_17945"
     CDS             <1..2022
                     /locus_tag="AC16CY_17945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355739.1"
                     /translation="GVAPQLGRAFTAGDAQGAQVVVISDALWRRRYGSDPGVVGRSIV
                     MNDVRHEVVGVAPKAFAFLARDVDYWIPMRLPPQLANTRNSHFLTVVARLKPGVAVDA
                     AAREMKSIAAQLTVEYPDSNRDVGAVIVPVREQVLGDTRVEVLVLTIAAASIVLIACA
                     NLASLLLSRAWARRGEYAVRLALGATRGRLIRQIVTEALCLSIIGGVLGLGIPLLASP
                     MVERFVPVGLQLPAMIFDWPLLAFAGVLSVSTGLLFSLGPALQSSRASTAEALQQHAR
                     GAVGAGTTGLFRDGLVVLQVAATVVLLVAAGLMLRTLANLNAIELGFNPDRLLTMQVS
                     LPQPKYADAAKRASFVDRVVAGIRAQPGVGGAAFGSTLPFQSIGNTQWFTIEGRQDVP
                     GEIRDTLYRVGTSDYLRTLGAVVVEGRLIDERDAAGAPLATVVNQTMAQQFLPNGSAL
                     GRRIRFEPEGPWFTIVGVVRNVLERGYEQEDKPAVYVSAPQVDGNPANLVVRVAADPL
                     AQAPAVQRVIRSVDPDQPVRLIRSMSDVVGSTVADRQQHTALLIAFGGLAMLIASIGL
                     YGLLAQSVSARSREIGLRIALGATWNSVVTMVMSRGIVLTAVGVAVGVLGAWMFTRVM
                     QSLLYGVNAGDPTTFAIVLGLLATVAVIACAIPAIRAARVDPMVVLREQ"
     gene            complement(2041..2988)
                     /locus_tag="AC16CY_17950"
     CDS             complement(2041..2988)
                     /locus_tag="AC16CY_17950"
                     /inference="COORDINATES: protein motif:HMM:NF052887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8021 domain-containing protein"
                     /protein_id="HIR3355740.1"
                     /translation="MALVKSIVTLLFIIGFLVSAAVPRASAQGACDRACLTGFLDGWF
                     KGLVNNSVSGVPAAKDVKITQNGHITTLAGTFWDSAASVPYRWDIANPRLGDTAAEAV
                     ISNADGTMTMLSVRLKVKGGAITEIETIKANRGEADGLWGPETLLKKGVSPALQLSIR
                     EAERDSYYRLIAAAEGYWRAFQTNGTPDYHPADLMPDTERFENGLQTTGLVRNGAYVS
                     AAAGFDAGRFLGRNLWDRRYPVVDEERGIVLSMVRFGLKDGQKSQSTSTANSRIVAEF
                     FSVKRGLIQEVHAVLFNVPDTLPAQWPADYGPGRGGAGW"
     gene            complement(3037..4074)
                     /locus_tag="AC16CY_17955"
     CDS             complement(3037..4074)
                     /locus_tag="AC16CY_17955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3355741.1"
                     /translation="MRNHTRAVAPVAALFVLVAMGAAAADDPNNVTGETGLILIDKRG
                     NHVRFFDPRTFAPQGFFSTGPLAPHDLAISPDHRTAYIPLYGDGIYRKNPNPGRSILV
                     VDLASQKVTDTIDVSPHQAPHGIQVDAKGTLYVVADISKTLLVIDPQARKVESAVDVE
                     GTGHWLAVLPDASKAYVANQTDVEFISVVDLKTRRMIGRIPSKGGTRGIVASPDGRRI
                     YAMEGGEPDVLVIDTATDTVVDNVRLQGHSQSGYKLRISPDGRTIVTCGSGGGSASFI
                     NIIDTANLHGPQAVLQAGRNSMGFAFAPDGKTVLVANDGDGTVTVIDVAARRVVSTFK
                     GGVGIESLSYF"
     gene            complement(4268..5104)
                     /locus_tag="AC16CY_17960"
     CDS             complement(4268..5104)
                     /locus_tag="AC16CY_17960"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR3355742.1"
                     /translation="MHPSSIEPTASDGRSLSRYFLLLFGLCLPVWVIGAIFDLELFPG
                     FKLFQAGLAMPMIASLLLTYRERRWAGITALLRRTCDAGNIKPRIWFLPIFFVYPSLG
                     FINYWILRATGADIPAPTLSLLGFLGYGTVFFMTFGEELGLSGYAMDPMQKRYSALVT
                     GLLLGLVWAGYHIPGFVISGYYSAGWILWHAIYTVTTRVLFVWIYNNSGKSLFSMALC
                     HWTFGLFWSLWPQDNLQRAVPFYNPKLTAVTAVFYVLIVVYLWGPRTLAQFRFARPRH
                     AT"
     gene            complement(5282..7375)
                     /locus_tag="AC16CY_17965"
     CDS             complement(5282..7375)
                     /locus_tag="AC16CY_17965"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3355743.1"
                     /translation="MRQTRGIYQLSGALVAVLAIVCAHLVAQQKSAVPGFTDADWPRF
                     AGDYAGTKYSKLTQINASNVSTLVQAWTFQGVGTQQTPIVVNGVMYASTPSGVVALDG
                     ATGALIWRYGATPPAGGGRGGGRGAGGGRGAPPAAQPESTDGAGQDGATPAQPAARGG
                     GPGGRGAATPGGGGRGGAVPTAEGPGGAPSSRGVAYWPGDGAVQPRILMIVGRRLVAL
                     NAATGALDTSFGAGGFIDTGVNWGGVPLVFKNIVVVGANNGEVTQGPAPGDTRAFDAR
                     TGEKLWSFVSVAQPGDPNHKTWQDEGWKNRQGVNHWGWYFTLDEQREILYTVFGSPAG
                     NYWGGDRPGANLYANSVVAVDVNTGKYLWHFQTVHHDLWDSDQPSPPTLVDATVSGKR
                     VPALGLIGKTGWMFLLDRTTGTPLFGVEERPVPKGDVPDEWYSPTQPFPVKPPALGRI
                     DFKKEDLVTAADTTPEHEKACLALYEKAGGFYNAGPFTPFLFHEAGAPPRSTIQFPGN
                     AGTNWGGPAGDPSLGYVFAFTQDAALTGWVEKKREGGNYGSGNGSPQPYDRGSINGPG
                     PYSGFAASMGPGLPNGPCQKPPWGRLTAVNAGTGEFAWQITLGVTDGLPPEKMNTGRG
                     GSAGPIVTAGGLVFIGTTSDARFRAIDSRTGKELFVTRLNATGTATPMTYQGRNGKQY
                     VVQAAAGTLNAFALP"
     gene            7511..9367
                     /locus_tag="AC16CY_17970"
     CDS             7511..9367
                     /locus_tag="AC16CY_17970"
                     /inference="COORDINATES: protein motif:HMM:NF012403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIR3355744.1"
                     /translation="MGDLTSRELDAHAAAFAQILATTGDAEVAFREAATLANGLFPHQ
                     VEGVAFLLGRRRAILADDMGLGKTRQAIVALRHTAPDGQVLVVCPASVKRNWAREIAL
                     AGAEGGVHIIDGTGRQQVPRETRWVIANYDILGRHVDAIAAVPWAALIFDEAHYLKNH
                     TSARSKRAREIASRAAAIGEPPVYLLTGTPLTSRPRDLFVLLQLAGHPLGRSFLSFAK
                     RYCAAEKTQYGWKTDGASNLEELTVQLHGTMLRRAKEEVLSLPPKLRTWLPTTVAAGI
                     ASREIREVMDLLVGRATGRSEAGDQRSRIRLLALLTRARQKLAAAKYETTLDLARGAV
                     EQGEKVIVFSCFDEPLQRLKKAFGSAAVVVTGKVASDKRQPLVDRFQQDDSVRVFLAN
                     IIAGGTGFNLTAATQVVFNDLDWVPANHWQAEDRAYRLGQTRTVNVAYVVAVRTIDEF
                     VQAVLETKTALVRSVVEGEALLADNGGAVLEELERAIAALSPGLADAGPDGGTEEIMD
                     ELVRVATEQWRSRHVSTRSRETRATDPATIRSLRAALEVLARVLAGPKGQRFKTASSS
                     KPGSHYFIDIDGSDVTCSCPGFEYRGQCKHAREIKNALASGKSPPAEYAVVT"
     gene            9396..12101
                     /locus_tag="AC16CY_17975"
     CDS             9396..12101
                     /locus_tag="AC16CY_17975"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355745.1"
                     /translation="MAIPPGTRLGPYEVIAAIGVGGMGEVYRARDAKLNRDVALKILP
                     EAFAADPDRLARFTREAQTLAALNHANIAQIHGLEEHPPLRALVMELVEGPDLSQRIA
                     QGPIQFDEALAIARQIADALESAHDQGIVHRDLKPSNIKVRDDGTVKVLDFGLAKAFD
                     GAGATTPSSMSPTLASPAVTGVGMILGTAAYMAPEQAKGKSVDKRADIWAFGVVLYEM
                     LTGRALFEGETTSEVLAAIIMREPDLSTLPATLPAPVRQLIGRCLIREPKLRMRDIGE
                     ARLALAAPGSGDVPAADAGPARPQRAGAARWVAVVLAIALAATGFALWRGTTAAPRRG
                     VIRYEVRPPDGTALALDSRPNLSVSPDGSTLVFAGRVEGTSRLYVRTPDDLMPRALPG
                     TEGATHPVISPDGRQVAFFAGAVLKRASFDGAVSEVISLGGETDAPRRGVTWLTNDTL
                     VYSPLGVSGLFSIASTGGTPRPLTTLDQKKGERTHRWPEALPGGKAVLFTVGSLASPD
                     SYDGSTIEAVDVATGARTVVLQGASSARYAATGHLLFVRGASVHAVPFDVDRLTTSGT
                     PVQVLQGVNGDSTTGAVHFSVAADGTMAYTPGSAQGGSNRLMWVDRTGMAQAIPLPRG
                     LYFDPRLAPDGTRVAVAWQTASAGSSDIWVSDLTRNTFTRLTFSSRASTPVWSRDGKT
                     IYYAYIEPTEQKTTIMRKPADGSRDEEPVVVLGARVYVQDVDPTEQWALIDYTPLNYV
                     GDVMRVALKANAKPEPLVSTKFDDLEATLSPDGRWLAYVSDETGRFEVYVRDMSSGGG
                     RWQLSTMGGEEPHWSGDGRQLYYRNSTKLMSVSVGGQGALNPQAPAEVFDGVHDFRSD
                     SGLSYAVDPKGGRFLMIRLSDESAVSSIVVVLNWFDELRRLTAAR"
     gene            complement(12106..12654)
                     /locus_tag="AC16CY_17980"
     CDS             complement(12106..12654)
                     /locus_tag="AC16CY_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355746.1"
                     /translation="MLRVVLLRSGRPRVCSSEGFTLLDMIFVTALLSLLLSIAVPTLL
                     RGRSAANETAALATLRVVHTGQLSYSLSCGFGLWAANFQALEDPSGGYLPPDMTAVAA
                     PTKSGYTYTLQPGPRGLAPVTDCNGKAVAFDYYVTAVPLSYGNTGSRGFASNPQHVIW
                     QDTTGAAPLEPFTTAGTVTPIE"
     gene            complement(12863..13180)
                     /locus_tag="AC16CY_17985"
     CDS             complement(12863..13180)
                     /locus_tag="AC16CY_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009237184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIR3355747.1"
                     /translation="MLMSTTTAIEGRPVSKYLGVVTGEAIIGANIFRDLFASVRDIVG
                     GRSATYERALGEAREVAMGEMKARAEQLGANAVIGVDVDYEVLGANNGMLMVAVSGTA
                     VVC"
     gene            13310..14113
                     /locus_tag="AC16CY_17990"
     CDS             13310..14113
                     /locus_tag="AC16CY_17990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3355748.1"
                     /translation="MGEPTLQSQIDAANAYEALFVPALFAQWAQRVADAAHLRPGQRV
                     LDVACGTGVVAREVASRVGSAGHAAGIDASPGMIAVARRLAPAVEYHEGTAESLPFPD
                     RSFDAVVSQFALMFFGDRRKALQEMLRVLAPGGRLVVAVWDSLESMPAFALEVVLLER
                     MAGRLAADALRAPFVLGNRNDLATLFKNAGMASAEITTERGTARFPSIRVMVEADLRG
                     WLPLMGVILAEDQIGRILREAEQALGSYATADGRASFPLSAHLVTAKKT"
     gene            complement(14173..15747)
                     /locus_tag="AC16CY_17995"
     CDS             complement(14173..15747)
                     /locus_tag="AC16CY_17995"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3355749.1"
                     /translation="MRFRGWLTVVAIGCLMATTSVSTAGSPLIDAVKKQDLQAVRALL
                     KQKVSINATEADGFTALHWASQRNDLQLVDLLLGAGANATASTRYAITPLYLAAVNGN
                     AAIMERLLKSGADPNATAQEGQTMLMTAALSGKADAVRLLLTRGARVDTKEPYRGQTA
                     LMWAAAEGNTAAVDVLLEAGADLTLKSNGAFTPLLFAVRNAHIDTAVTLLKRGANVND
                     VASDGSSALSIATVNAFFELASVLLDHGANPNLPDPRASPLHTVAWLRRPGADGAAGV
                     GNTPVGTPQQTGKVTAIELARTLLEHGANPNTRVEWNEPRFGKEGGTARNPPNIRLGR
                     HLLSYRGATPFYVAAKNGDIELMRLLVQFKADPTITTLTGITPLMVAAGLDTWEGETP
                     GPFTGVPEAERLEAVKFAVSLGADVNARANFGDYPMEGDVEYTLLYYPHNIDTLLDLG
                     VGDPRWNGSTPLVGAIMSNQPSIVQYLIDQGADVRATTTLGWTPLNIAEGVFCCNAKK
                     EFPAAAAIIRKALGVN"
     gene            15796..>16709
                     /locus_tag="AC16CY_18000"
     CDS             15796..>16709
                     /locus_tag="AC16CY_18000"
                     /inference="COORDINATES: protein motif:HMM:NF019255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HIR3355750.1"
                     /translation="MRPAEAGHYLLLAASAVLMLTALWRADSVVAQVRVAPATAASSS
                     AAVLKQYCATCHNERLKSGGFVIDPAGVADVSAGADGWEKIVRKLRTQSMPPPGAPRP
                     DAAGYDRVATFLETELDRAEAARPHVGKLPLTHRLSRTEYRNAVRDLLALESLPREVS
                     VDYLLPPDNISSGFDNISDLLFISPSNMERYLDAARKISRLAVGDPAMPVMVNIHCLD
                     PEHPQDERVDELPFGTRGGIAVRSEFPVDGTYVVRVDVGNAQGHELEILVDGERVARR
                     SLGGAGRGRGAPAVDTPPGQPDPADPDPA"
BASE COUNT         2738 a         5487 c         5606 g         2878 t
ORIGIN      
        1 ggcgtggcac cacagctcgg tcgcgcgttc actgccggcg acgcacaggg tgcccaggtg
       61 gtcgtcatca gtgacgcgct ctggcggcgt cggtatggaa gcgatccagg ggtcgtcggc
      121 cgctcgattg tcatgaacga tgtccggcac gaggttgtcg gggtggcgcc gaaggcattc
      181 gctttcctcg ctcgcgatgt cgactactgg attccgatgc gactgccgcc acaactggcc
      241 aacacgagaa actcgcattt cctcactgtc gtcgcgcgcc tcaagccggg cgtcgcggta
      301 gacgccgccg ctcgcgagat gaagtcgatc gcggcgcaac tgaccgtgga gtatccggac
      361 tccaatcgag atgtcggggc tgtcatcgtg cccgtgcgcg agcaggtcct cggcgataca
      421 cgcgtcgagg tgctggtgct cacgatcgcc gccgcgtcca tcgttctgat tgcatgcgcg
      481 aaccttgcga gcctcctgct ctcgcgtgcg tgggcgaggc gcggcgagta cgcggtgcgt
      541 ctcgcgctgg gcgccactcg agggcggttg atccggcaga tcgtcaccga ggcgctgtgt
      601 ctgtcgatca tcggcggtgt tctcggactg gggattccgt tgctggccag cccgatggtc
      661 gaacgattcg tgcccgtcgg tctgcaactg cctgcgatga tcttcgactg gccgcttctc
      721 gcgttcgcgg gggtgttgtc ggtctcgacc ggcttgctct tcagcctcgg gcccgccctc
      781 cagtcgtctc gtgcctccac cgcagaggcg ctccagcagc atgcccgggg tgccgttggg
      841 gccggaacga ccggcctgtt tcgggatggc ctggtcgtgc tacaggttgc cgcaacagta
      901 gtgctcctgg tcgcggcggg actcatgctg aggacgctgg ccaacctcaa tgcgatcgag
      961 cttggcttca accccgatcg cctgctcacg atgcaggtgt cgctgccaca accgaagtac
     1021 gcagacgccg cgaagcgcgc gtcgttcgtc gatcgcgtgg tcgctggcat ccgcgcacag
     1081 cctggcgtcg ggggcgcggc gtttggttcg acgctgccgt tccagagcat tggcaacacg
     1141 cagtggttca cgatcgaagg ccgccaggac gtgcctggag aaatccgcga cacgctgtac
     1201 cgcgtcggca cgtcggacta tttgcggacg ctgggcgcgg tcgtcgtcga gggccgcctg
     1261 atcgacgaac gtgatgccgc tggcgcgccc ctggcgacag tcgtgaacca gacgatggcg
     1321 cagcagttcc ttccgaatgg gagcgcgctc ggacgtcgga ttcggttcga gccggaggga
     1381 ccgtggttca cgattgtcgg tgtcgtgcga aacgttctcg agcggggcta cgagcaggaa
     1441 gacaagcccg cggtgtacgt gtctgcgcca caggtggacg gcaatcccgc aaacctggtt
     1501 gttcgtgtcg ccgccgaccc gctcgcgcag gcgcccgccg tgcagcgagt gattcggagc
     1561 gtcgatcccg atcagccggt gcgcttgatc cgctcgatgt ctgacgtcgt cgggtcgacc
     1621 gtcgccgacc gccagcagca cacggcgctg ctgatcgcgt ttggcgggct cgcgatgctc
     1681 atcgcgtcga tcgggcttta cgggttgttg gcgcagtccg tgtcggcgcg aagccgtgaa
     1741 atcggattgc gcatcgccct tggcgccacc tggaacagcg ttgtcacgat ggtgatgtcg
     1801 cgggggattg tcctgaccgc tgtcggcgtg gccgtgggcg tcctcggggc gtggatgttc
     1861 acgcgagtca tgcagtcgct gctgtatggc gtgaacgccg gcgacccgac gacgtttgcg
     1921 atcgtgctcg gacttctcgc gacggtggcg gtcattgcct gcgcgattcc ggccatacgc
     1981 gcggcccgcg tggatcctat ggttgtgctc cgcgagcagt aaggcagccg gagttcgagg
     2041 tcaccagccc gcgccgccgc ggccgggtcc atagtcggca ggccactgtg ccggcaaggt
     2101 atcgggaaca ttgaacagca ccgcgtgcac ttcctgaatc aagcctcgct tgacggaaaa
     2161 gaactccgcg acgatgcggc tgttcgccgt ggacgtactc tggctcttct ggccgtcctt
     2221 gagaccgaac cgcaccatcg acaacacgat gccgcgttct tcgtccacca cgggatagcg
     2281 gcggtcccag aggttgcggc caagaaaacg gcctgcatcg aacccggcgg cggcggacac
     2341 atacgcgccg ttgcgcacga ggccggtggt ctgcagcccg ttctcgaagc gctcggtatc
     2401 gggcatgaga tcggcagggt ggtagtccgg cgtgccattg gtctggaacg cgcgccagta
     2461 tccttccgcc gcggcgatca acctgtaata ggaatcgcgc tcggcttcgc gaatcgacaa
     2521 ctggagcgca ggagacacgc ccttcttcaa caaggtctcc ggtccccaca gcccatccgc
     2581 ttcacccctg ttggccttga tggtctcgat ctccgtgatc gccccgccct tcaccttcag
     2641 ccgcacggac agcatcgtca tggtgccgtc ggcgttgctg atgacggcct cggcggccgt
     2701 gtcacccaac cgcggattgg cgatgtccca ccggtaaggc acactggcgg cactgtccca
     2761 gaacgttcca gccagcgtgg tgatgtgacc gttctgcgta atcttgacgt ctttcgctgc
     2821 ggggacgccg ctgacgctgt tgttcacgag tcctttgaac cagccgtcga gaaatccggt
     2881 gaggcaggcc cggtcgcacg ctccctgtgc gctggcgcgg ggaaccgccg ctgacaccag
     2941 gaagccgatg atgaacagga gcgtcacgat gctcttcacg agtgccatga acaccctcca
     3001 ggttgggggc agggagtata gcgcggcgtc attctgctag aagtagctga ggctttcgat
     3061 gcccacgccg cccttgaacg tcgataccac ccgcctggcc gcgacgtcga tgacggtcac
     3121 cgtgccgtcg ccgtcgttgg ccacgagcac cgtcttcccg tccggggcga acgcaaagcc
     3181 catggagttc ctgccggctt gaagcacggc ctgcggtccg tgcaggttcg ccgtgtcgat
     3241 gatgttgatg aacgaggcgc tgccgccgcc gctcccgcac gtgacgatgg tgcgcccgtc
     3301 cgggctgatc cgaagcttgt agcccgactg tgaatgcccc tgcaggcgaa cgttgtcgac
     3361 gaccgtgtcg gtcgcggtgt cgatgacgag gacatccggt tcgccgccct ccatcgcgta
     3421 gatgcgccgg ccgtccggag acgccacgat gccgcgtgtg ccgcctttcg acgggatccg
     3481 gccgatcatc ctgcgcgtct tcagatcgac gacgctgatg aactccacgt cggtctgatt
     3541 tgccacatag gccttgctgg cgtcaggcag gacggcgagc cagtggccgg tcccttcgac
     3601 gtcgacggcg ctctccacct tgcgcgcctg tggatcgatg acgagcagcg tcttgctgat
     3661 gtcggcgacc acgtacagcg tgcctttggc gtcgacctgg atgccgtgcg gggcctgatg
     3721 cggtgagaca tcgatggtgt cggtcacctt ctgagacgcc agatcgacca cgagaatcga
     3781 gcggccaggg ttggggttct tgcggtagat gccgtctcca tagaggggga tgtaggcggt
     3841 gcggtggtcc ggcgagatcg ccagatcgtg gggcgccagc ggccccgtcg agaagaatcc
     3901 ctgcggcgca aacgtcctgg gatcgaagaa ccggacgtgg ttgccgcgct tgtcgatcag
     3961 gatgagtccc gtttcgccgg tgacgttgtt cgggtcgtcg gccgctgcgg cgcccatcgc
     4021 aacaagaacg aagagtgccg ccacgggcgc gaccgctctg gtgtgattcc gcatcggtgc
     4081 atcctcctct ggccgaccct ccgaggttca gggcagggag tatagcagtg ggccggtgtg
     4141 tgcccggccg gtagcgggcc atgacagcca tgagggcggc ctcgaaagat gactcccgat
     4201 cccataatcg taggatgcga ccaagtgcgc aaacgaagat gcagtcgccc tgggacaggg
     4261 tggggcgtca ggttgcgtga cgcggacgcg cgaacctgaa ctgcgccagc gtccttggcc
     4321 cccaaaggta gacaacaatc agtacataga agacggcggt cactgccgtg agcttggggt
     4381 tgtagaacgg gaccgccctt tgaaggttgt cctggggcca taggctccag aacaggccaa
     4441 aggtccaatg gcacagggcc atcgaaaaga ggctcttgcc agagttgttg tagatccaga
     4501 cgaacaagac gcgggtggtc actgtgtaga tggcatgcca gaggatccac cccgccgagt
     4561 agtagccgct gatgacgaag ccagggatgt ggtagccggc ccaaacaaga cccagcagca
     4621 gacctgttac gagcgcgctg tatcgcttct gcattgggtc catggcatac cccgaaagac
     4681 cgagctcctc cccaaacgtc atgaagaaga cggtgccgta acccaggaag ccaagcagag
     4741 agagagtggg cgcggggatg tcggcgccgg ttgcccgaag gatccaatag ttgatgaagc
     4801 caaggctggg atagacgaag aagatcggaa ggaaccagat tctgggcttg atattgccgg
     4861 catcgcaggt ccttcgtagc agagcggtga tgcccgccca gcgtcgctca cggtaggtca
     4921 ggagcagcga tgcgatcatc ggcatcgcca gacccgcctg gaagagtttg aacccaggga
     4981 acagctcaag gtcgaagatg gctccgatta cccaaacggg caggcagaga ccgaataaga
     5041 gcaggaagta gcgggacaag gatctgccgt cggatgctgt gggttcgatc gacgaaggat
     5101 gcatgggtac ctgggcgtag gaagcatagg ctccttggcg ggcaactggc gagttgacga
     5161 aaccccagtt ccgccggcgc gagctggacg cgacagcagg tcaccgacca gcaatggcgc
     5221 gtgctgcgcg agcgtctggc agctgtcgaa ggaggtggcc ggtcctgcgg cgatttcctg
     5281 atcagggcag cgcgaacgcg ttcagcgttc cggccgccgc ctgcaccacg tactgcttgc
     5341 cgttgcgccc ctgatacgtc atcggcgtcg ccgtgccggt ggcgttcagc cgcgtgacga
     5401 acagttcctt ccccgtcctg gaatcgatcg cccggaagcg cgcgtcggac gtcgtgccga
     5461 tgaacacgag gcctcccgcg gtgacgatcg gacccgccga tccgccgcgg ccggtattca
     5521 tcttctcggg cggcaatccg tccgtgacac cgagcgtgat ctgccaggcg aattcgccgg
     5581 tgccggcgtt gacggccgtc agccgtcccc acggtggttt ctgacacggc ccgttcggca
     5641 gccccggccc catggacgcc gcgaacccgg agtaggggcc gggcccgttg atgctgccgc
     5701 ggtcgtacgg ctgtggcgag ccgttgccgc tgccgtagtt gcccccctcg cgcttctttt
     5761 ccacccagcc ggtgagcgcg gcgtcctgcg tgaaggcgaa gacgtagcca agcgatggat
     5821 cgcccgccgg cccgccccag ttcgtgccgg cgttgcccgg aaactggatc gtgctcctcg
     5881 gcggcgcgcc cgcctcgtgg aacaggaacg gcgtgaaggg tcccgcgttg tagaagcctc
     5941 ccgccttttc gtacagcgcg aggcacgcct tctcgtgctc cggcgtcgtg tcggccgcag
     6001 tgaccagatc ctccttcttg aagtcgatcc ggccgagggc cggcggcttc accgggaacg
     6061 gctgcgtggg cgagtaccac tcatctggca catcgccctt cggcaccgga cgttcctcga
     6121 cgccgaagag cggcgtgccc gtcgtccgat cgagcaggaa catccacccg gtcttgccaa
     6181 tcaggccgag ggcgggcaca cgcttgccgc tcaccgtcgc atccacgagc gtcggcggcg
     6241 acggctgatc ggagtcccag agatcgtggt ggaccgtctg gaaatgccac aggtacttcc
     6301 cggtgttcac gtccacggcc accaccgagt tggcgtagag gtttgcgccc ggccggtcgc
     6361 cgccccagta gttgccggcg gggctgccga acacggtgta gaggatctcg cgctgctcgt
     6421 cgagcgtgaa gtaccagccc cagtggttca ccccctgccg gttcttccac ccctcgtcct
     6481 gccaggtctt gtgattcgga tcgcctggct gcgcgaccga cacgaagctc cacagcttct
     6541 cgcccgtgcg cgcgtcgaac gcgcgagtgt ctcccggcgc tggaccctgc gtgacctcgc
     6601 cgttgttggc gccgacgacg acaatgttct tgaagacgag cggcacaccg ccccagttga
     6661 cgccggtgtc gatgaagcct ccggcgccga acgaggtgtc gagcgctccc gtcgcggcgt
     6721 tgagcgcgac gaggcggcgc ccgacgatca tcaggatgcg cggctgcact gcgccgtccc
     6781 cgggccagta ggcgacgccg cggctcgacg gcgcaccccc cgggccttcg gccgtcggca
     6841 ccgcgcctcc gcggccccca ccgcccggcg ttgccgcgcc acgaccaccc ggtccgccgc
     6901 cccgcgcggc gggctgggcc ggcgtggcgc catcctgtcc ggcgccatcc gtggactcag
     6961 gctgcgccgc gggcggagcc cctcgtccgc cgccggcacc gcgcccaccg cccctgccac
     7021 cgcccgcggg cggcgtggcg ccgtagcgcc agatcagcgc gccggtcgcg ccgtcgagcg
     7081 ccactacgcc ggatggcgtc gaggcataca tcacgccgtt gaccacgatc ggagtctgct
     7141 gcgtccccac gccctggaac gtccaggcct gaacgagggt ggacacgttc gaggcgttga
     7201 tctgcgtgag cttcgagtac ttcgtcccgg cgtaatcgcc cgcaaagcgg ggccagtcgg
     7261 catcggtgaa gccgggcacc gcgcttttct gttgcgcaac gagatgcgcg cagacgatgg
     7321 ccagcaccgc cacgagcgcg ccggacagtt ggtagatccc tcgcgtttgc ctcacgacca
     7381 tgtctcctgc ctccgccggc cgttccggcc gagtgggcgg tggaagctta ccgcaaccag
     7441 cgtccctccg gcgcgcttcg tcgtgccttc ctttttgccc gtcgtgtcag cacactgttg
     7501 ctgaggcaac atgggcgatc tcacgtcacg tgagttggac gcgcacgcgg cggcgttcgc
     7561 gcagatcctc gcgacgaccg gcgatgccga ggtcgccttc cgcgaggcag ccacgctggc
     7621 gaacggcctc ttcccgcacc aggtcgaggg cgtggcgttc ctgctgggcc ggaggcgcgc
     7681 gattcttgcc gacgacatgg gcctcggcaa gacccgccag gcgattgtcg cattgaggca
     7741 caccgcgcca gacgggcagg tgctcgtcgt gtgcccggcg tcggtgaagc gcaattgggc
     7801 gcgcgaaata gcgctggctg gcgccgaagg cggcgtccac atcatcgacg gcacgggcag
     7861 gcagcaggtc cctcgcgaga cgcgctgggt catcgccaac tacgacattc tcggccgtca
     7921 cgtggacgcg atcgccgcgg tgccctgggc ggctctgatc ttcgacgaag cgcactacct
     7981 gaagaaccac acgagtgcgc gcagcaagcg tgcccgcgag atcgccagcc gcgccgccgc
     8041 cattggagag ccacccgtct atctgttgac cggtacgccg ctgaccagcc ggccgcgcga
     8101 cctgttcgtg ctactgcagc tcgccgggca tccgctcggc cgcagctttc tctcgttcgc
     8161 gaaacgctac tgcgccgcgg agaagaccca atacggctgg aagaccgacg gtgcctcgaa
     8221 cctcgaagaa ctgacggtac agctccacgg gacgatgctg cggcgcgcca aggaggaagt
     8281 gctctcgctt ccgccgaagt tgaggacgtg gctcccgacg actgtcgcgg caggcatcgc
     8341 cagccgcgag atacgcgagg tcatggacct gctcgtgggg cgcgccaccg gcagatccga
     8401 agccggggat cagcgatccc gcattcgcct gctggcgctc ctgacgaggg cgaggcagaa
     8461 actcgccgcc gcgaagtacg agacgacgct ggatctggct cgaggcgcag tcgagcaggg
     8521 cgagaaggtc atcgtgttct cgtgcttcga cgagccgctg cagcggctga agaaagcgtt
     8581 cggctccgcc gccgtcgtcg tcactgggaa ggtggcctcg gacaagcgac agccgctcgt
     8641 cgatcgcttt caacaggacg acagcgttcg cgtattcctg gcgaacatca tcgccggcgg
     8701 aacgggtttc aacctgaccg ccgcgacaca agtggttttc aacgacctcg actgggtgcc
     8761 ggcgaatcac tggcaggccg aggaccgcgc ctaccgcctc ggacaaaccc ggacggtcaa
     8821 cgtcgcctac gtggtcgctg tacggacgat cgacgaattc gtacaggccg tgctcgagac
     8881 gaaaacggcg ctcgtgcgat cggtcgtcga gggcgaagcg cttctcgccg acaacggtgg
     8941 tgcggtactc gaagagctgg agcgcgcgat cgctgcgctg tcgccggggt tggccgacgc
     9001 gggtccggac ggcggcaccg aggagatcat ggatgaactg gtgcgggttg cgaccgagca
     9061 gtggcgcagc cggcacgtct cgacgcgttc gcgtgagacc agggccaccg atcctgccac
     9121 gatacgcagc ctgcgggctg cgctggaagt gcttgcgcgc gtgctcgcag gcccgaaggg
     9181 gcagcggttc aagacagcca gctcttcgaa acccggcagc cactacttca tcgacatcga
     9241 cggatccgac gtcacctgct cgtgtcccgg cttcgagtac cgcggccagt gcaagcacgc
     9301 acgggaaatc aagaacgcgc tcgcatcggg caagtctccg cccgcggagt acgcggtcgt
     9361 cacgtgagca cgcgcgggta taatccgcga cgggcgtggc gattcctccc ggaacgagac
     9421 tcgggccgta tgaagtcatc gccgcgatcg gcgtcggcgg catgggtgag gtctatcgcg
     9481 cgcgggacgc gaagctcaat cgcgacgttg cgctgaagat cctgcccgag gcgttcgcgg
     9541 ccgacccgga tcgcctcgcg cggttcacgc gcgaagcgca gacgctggcc gctctcaacc
     9601 acgccaacat cgcccagatc cacgggctcg aggagcaccc gccgctgcgg gctctggtga
     9661 tggaactggt cgagggcccg gatctctccc agcgcattgc gcaaggtccg attcagttcg
     9721 acgaggcact ggcgatcgcc cgccagatcg ccgacgcgct cgagtccgca cacgaccagg
     9781 ggatcgtgca ccgcgatctc aagccgtcga acatcaaggt ccgcgacgac ggcaccgtca
     9841 aggtgctcga tttcggcctc gccaaggcat tcgatggggc cggcgccacg actccgtcgt
     9901 ccatgtcgcc gacgctggcc agccccgccg tgaccggcgt cggcatgatc ctcggcaccg
     9961 ccgcgtacat ggcccccgag caggcaaagg gcaagagcgt cgacaaacgc gccgacatct
    10021 gggcgttcgg cgtcgtgctc tacgaaatgc tgacggggcg ggcgctgttc gaaggcgaaa
    10081 cgacgtccga ggtgctcgcg gcgatcatca tgcgcgagcc ggatctctcg acccttccgg
    10141 ccacgttgcc cgcgcccgtc aggcagctga tcggacgctg cctgatcagg gaaccaaagc
    10201 tgcgcatgcg tgacatcggc gaagcgcggc tggctcttgc cgcgccggga tccggcgacg
    10261 tacccgcggc cgatgccggg cctgcgcgtc ctcagcgcgc cggcgctgct cgttgggtgg
    10321 cagtcgtcct ggcgatcgcc cttgccgcga cgggattcgc cctgtggcgc gggaccaccg
    10381 ccgcgccacg caggggagtg atccgctacg aggtgcgtcc tccggatggg acggcgttag
    10441 cgctcgattc gcggcccaac ctgtcggtgt cgccggacgg ctcgacgctg gtgttcgccg
    10501 ggagggtcga gggaacgtcg cgcttgtacg tccgtacgcc tgacgacctg atgcctcgcg
    10561 cgttgcctgg aaccgagggc gcaacccatc ccgtcatctc tccggacgga cgccaggtcg
    10621 cattcttcgc gggcgcagtc ctgaaacgcg cgtcgttcga cggcgcggta tcggaggtga
    10681 tcagcctcgg cggcgagacc gacgccccgc gccgaggcgt gacgtggctg accaacgata
    10741 cgctggtcta ctcgccgctc ggcgtcagcg gactcttctc catcgcctcc acgggcggaa
    10801 cgccgcggcc cctgacgacg ctcgaccaga agaagggcga acgcacgcat cgatggcccg
    10861 aggcgctgcc gggtggcaag gcggtgctct tcacggtcgg cagcctcgcg agcccggaca
    10921 gctacgatgg ctcgacgatc gaggcggttg acgtggcgac cggtgcgcga acggtcgtgc
    10981 tgcagggcgc cagttcggcg cggtacgcgg cgaccggcca cctcctgttc gtgcgcgggg
    11041 cgtccgtgca cgcagttccg ttcgatgtcg atcgtctgac aacgagcggc acgccggtgc
    11101 aggtgctgca gggcgtcaac ggcgactcga ctacgggcgc cgtgcatttc tcggtcgccg
    11161 cggacggcac gatggcctac acgcccggca gcgcgcaggg cggttcgaac cggctgatgt
    11221 gggtcgatcg cacgggcatg gctcaggcga tcccgttgcc acggggtctc tacttcgatc
    11281 cgcgtctcgc ccccgacggt acgcgcgtgg ccgtagcctg gcagacggcg tcggcgggga
    11341 gcagcgacat ctgggtcagc gatctgacgc gaaatacgtt cacccgcctg accttcagca
    11401 gcagggcgtc gacgccggtg tggtcgcgcg acggcaagac gatctactac gcgtacatcg
    11461 agcccacgga gcagaaaacg accatcatgc ggaagcccgc ggacggcagc cgcgacgagg
    11521 agccggtcgt cgtcctcgga gcacgcgtct acgtccagga cgtcgacccc actgaacagt
    11581 gggccctcat cgactatacg ccgttgaact acgtgggcga cgtcatgaga gtggcgttga
    11641 aggcgaatgc caagccggaa ccgctggtgt ccacgaagtt cgacgacctc gaagccacgc
    11701 tctcaccgga cggacgctgg ctcgcgtacg tgtccgacga gaccgggcgg ttcgaggtgt
    11761 acgtacgcga catgtcgagc ggcggcggcc gatggcagct gtccacgatg ggcggcgagg
    11821 agccgcactg gtctggcgac ggtcgccagc tgtactacag gaacagcacg aagctgatgt
    11881 cggtctcggt cgggggacag ggcgccctga atccccaggc gccggccgag gtgttcgacg
    11941 gcgtgcacga cttccgatcg gactccggtc tcagctacgc ggtcgatccg aagggcggcc
    12001 gtttcctgat gatccggctg agcgacgaga gtgccgtctc ctccatcgtc gtcgtcctca
    12061 actggttcga cgagctgcgg cgattgacgg cagcgcggtg agccctcact caatcggggt
    12121 cacggtccct gcggtcgtga acggctccag gggcgcggcg ccggtcgtat cctgccagat
    12181 cacgtgctgt ggattgctgg caaacccacg actgccggtg ttgccgtagc tgaggggcac
    12241 cgcggtgacg tagtagtcga acgcgaccgc cttgccgttg cagtcggtca ccggagcgag
    12301 gccgcggggc cccggttgaa gcgtatacgt gtaaccgctc ttcgtcggtg ccgcgactgc
    12361 tgtcatatcc ggcgggagat acccgccact cgggtcctcg agcgcctgaa agttcgccgc
    12421 ccaaagaccg aagccgcacg agagggagta ggacaactgc ccggtatgca ccacacgcag
    12481 cgtcgcaagc gcagctgtct cgttcgccgc cgagcgaccg cgaagaaggg tcgggaccgc
    12541 gatcgacaac agcagtgaca agagggcggt gacgaagatc atgtcgagca gagtgaagcc
    12601 ttcgctcgag cacaccctcg ggcgcccgct ccgaagaaga acaacgcgca gcatatgccc
    12661 gtgtccttca gggcaacaac gatgccgcgc cagacacact atgcaaatgc gtgtggcgcc
    12721 cggaagggcc gacgatatgc aggtaccagg gacttcgacg cggactgtcc cgctgacagg
    12781 aattgtcaga cgtccctaaa acaaattgtc actgccacta actgacggct tgcacgaaaa
    12841 gacgctcgac gtcctcatcg tgtcagcaga cgacggcggt tccgctcacg gcaaccatca
    12901 gcatcccgtt gttcgcgccg agcacttcgt agtccacgtc gacaccgatg acggcgttgg
    12961 cgccgagctg ctcggcccgc gccttcatct cacccatcgc cacctcgcgc gcttcgccca
    13021 gcgcgcgctc gtaggtggcc gaccggccgc ccacgatgtc gcgaacgctc gcgaagagat
    13081 cccggaagat gttcgcgccg atgatcgcct cgccggtcac cacgccgagg tacttgctga
    13141 ccggtcgtcc ttcgattgcc gttgtcgtgc tcatcaacat gcgtcggctc cactctcgag
    13201 tgttctgtca acccgtcact gtcagacact gatacatacg ccgtttcaac ttgccgggtt
    13261 cagcggaaaa cggatagaaa tagcggctga ctcgccccga ggactcgaca tgggtgaacc
    13321 gacgcttcag tcgcagatcg atgcggccaa cgcgtacgaa gcgctgtttg tgccggcgtt
    13381 gttcgcacaa tgggctcaga gagtcgccga cgccgcgcac ctgcggcctg gtcaacgcgt
    13441 gctcgacgtg gcctgtggca cgggagttgt tgcgcgcgag gtggcgtcgc gagtcgggtc
    13501 ggccgggcac gcggccggaa tcgatgcgag tcccggcatg attgccgtgg ctcggcggct
    13561 cgcgcctgcg gtcgagtatc atgagggaac cgccgaatcc cttccattcc ccgatcggtc
    13621 cttcgacgcg gtcgtcagcc agttcgcgct catgttcttc ggcgaccggc gaaaggccct
    13681 ccaggagatg cttcgcgtcc tggctcccgg tggacggttg gtcgttgccg tctgggactc
    13741 tctcgagagc atgcccgcat tcgcattgga ggtggtgctg ctggagcgaa tggcgggccg
    13801 gctcgcggca gatgcccttc gcgcgccatt cgtgctcggc aatcggaacg atctggccac
    13861 gctgttcaag aacgccggca tggcctcagc cgagatcacg accgaacgcg ggaccgcgcg
    13921 gttccccagc attcgcgtca tggtcgaagc cgacctcagg ggctggttgc ccctgatggg
    13981 agtgatcctg gccgaggatc agatcggccg catccttcgg gaggccgagc aggctctcgg
    14041 ctcgtacgcg accgccgatg gacgagcgtc gttccctctg tcggcacacc tcgtcaccgc
    14101 gaagaaaacg tagcgctaat gtcgtgctgc ccttcaactc gaagagcggt tggagcgatt
    14161 tcctgcgcta gttcaattaa cgcccagcgc ctttcggatg atcgctgcgg ccgccgggaa
    14221 ctccttcttc gcgttgcagc agaagacacc ttcagcaatg ttcagcggtg tccaacccag
    14281 cgtcgtcgtc gcgcgcacgt cggcgccctg atcgatgagg tactgcacga ttgacggctg
    14341 gttggacatg atcgcgccga cgagcggcgt gctgccgttc catcgaggat cgccgacgcc
    14401 gaggtcgagc agcgtgtcga tgttgtgcgg gtagtagagc agcgtgtact cgacgtcgcc
    14461 ttccattgga taatcgccga agttcgcgcg cgcgttgacg tcggcgccca gcgacacggc
    14521 gaatttcacc gcctcgagcc gctccgcttc gggcactccg gtaaacggcc ctggcgtttc
    14581 accctcccag gtgtccagac ccgcggccac catcaagggc gtgatgccgg tcagcgtcgt
    14641 gatcgtcggg tccgccttga actggacgag cagccgcatc aattcgatat cgccgttctt
    14701 ggcggcgacg tagaacggtg tcgcgccccg gtagctgagg agatgacgcc cgagccggat
    14761 gttcggcggg ttgcgcgccg tgccgccttc cttgccgaag cgcggctcgt tccactcgac
    14821 ccgtgtgttg ggattggcgc cgtgctcgag gagtgttcgt gcgagctcga tggcggtgac
    14881 ttttcccgtc tgctgcggcg tcccaacggg cgtgttgccg acgccggcgg cgccgtccgc
    14941 gccgggccgg cgcagccacg ccacggtatg gagcggcgac gcacgcggat ccggcaggtt
    15001 cgggttcgcg ccgtgatcga gcagcaccga tgccagctcg aagaaggcgt tgacggtcgc
    15061 gatgctcagc gcgctcgagc cgtcggacgc gacgtcgttg acgttcgcgc cgcgtttcag
    15121 cagcgtcacg gcggtgtcga tgtgcgcgtt ccgcacggcg aagagcagcg gtgtgaaggc
    15181 gccgttcgat ttcagtgtga ggtcggcgcc cgcttcgaga aggacgtcga ccgcggcggt
    15241 gttgccttcg gccgccgccc acatcagcgc cgtctgacct ctgtacggct ccttcgtatc
    15301 gaccctggcg ccgcgcgtca gcaacagcct cacggcgtcc gccttccccg acagcgccgc
    15361 cgtcatcagc atcgtctgac cttcctgcgc ggtcgcgttc gggtcagcgc cggatttcag
    15421 caggcgctcc atgatcgcgg cgttgccgtt gacggccgcg agatagagcg gcgtgatggc
    15481 gtagcgcgtt gacgccgtgg cgttcgcgcc ggcgccgagg aggagatcga cgagctgcag
    15541 gtcgtttcgc tgcgacgccc agtggagggc cgtgaagccg tccgcttcgg tggcgttgat
    15601 gctgaccttc tgtttcagca gggcccgaac cgcctggagg tcctgtttct tgaccgcgtc
    15661 gatcaacggt gacccagccg ttgagacgga tgtcgtcgcc atcaggcagc cgatggcaac
    15721 gaccgtgagc cagccgcgaa accgcatacg gcgttaatat aactccgtat ttgaatgtcc
    15781 tgtagaggtt tgcgtgtgcg tccggctgaa gccggacact acctacttct ggcggcgagc
    15841 gccgtgctga tgctgacggc cctttggcgc gccgacagcg tcgtcgcaca ggtccgggta
    15901 gcgccggcca cagccgcgtc ttcgtctgcc gctgtcctca agcagtactg cgcgacgtgc
    15961 cataacgagc gcctgaaaag cggcggcttc gtcatcgatc cagccggcgt ggctgatgtc
    16021 agtgcgggcg ccgacggctg ggagaagatc gttcgcaagc tgcggacgca gtcgatgccg
    16081 ccaccggggg cgccgcgtcc ggacgcggcc ggctacgacc gcgtggcgac gttcctcgaa
    16141 acagagctgg accgcgccga agccgcgcgg ccgcacgttg gcaagctgcc gctgacgcat
    16201 cgactcagcc gcacggagta tcggaacgcc gtgcgcgatc tgctcgcgct cgagtcgctg
    16261 ccgcgcgagg tcagcgtcga ctacctgctg ccgccggaca acatcagcag cggcttcgac
    16321 aacatctccg acctgctgtt catttcgccg agcaacatgg agcgctatct cgacgcggcg
    16381 cggaagatca gccgcctcgc tgtcggcgat cccgccatgc cggtgatggt gaacatccat
    16441 tgcctcgatc ccgagcatcc acaggacgag cgcgtggacg aactgccatt cggcacgcgc
    16501 ggcggcattg ccgtgcggag cgaattcccc gtggacggca cgtatgtcgt ccgtgtggac
    16561 gtgggcaatg cgcagggaca cgaactcgag atccttgttg acggcgaacg cgtcgcccgg
    16621 cgatcgctcg gcggcgccgg ccgcggccgc ggcgctcccg cagtcgatac tcctcccggt
    16681 caacccgatc cggccgatcc cgatccggc
//
LOCUS       DBNGSH010000783         2894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2972608, whole genome shotgun sequence.
ACCESSION   DBNGSH010000783 DBNGSH010000000
VERSION     DBNGSH010000783.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2972608"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC16CY_18005"
     CDS             complement(<1..235)
                     /locus_tag="AC16CY_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355751.1"
                     /translation="MADLMKPLHDLFFWVSEFPTSIAIRESQYVSPYLTVSHVISMCI
                     FAGLIVMMDLRLLGVGNMRTPFSEVQKRLFPWQM"
     gene            complement(275..820)
                     /locus_tag="AC16CY_18010"
     CDS             complement(275..820)
                     /locus_tag="AC16CY_18010"
                     /inference="COORDINATES: protein motif:HMM:NF013487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR3355752.1"
                     /translation="MKRVYGLVAITALFISCTQAPAPAPAPAPAAPQPKVYGTLAQVM
                     QGIPFPNSNIIFDTQTNDPGAPQKSGGAGAAATQQYSGVYGGWVAVENAAIALQETAN
                     LILIPGRMCQNGKPVPVDQADFQKWAQGLADAGAATLKAAKAKNIDQMIEVSGTVSDA
                     CLACHEKYRDTPKAPDDRCMP"
     gene            1040..2425
                     /locus_tag="AC16CY_18015"
     CDS             1040..2425
                     /locus_tag="AC16CY_18015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3355753.1"
                     /translation="MQDLTTGSLTGHLLKTTSYMLVSMLFQTLYVLVDLYWVGRLGTE
                     AVAAVAVSGNLTFVVLAATQLLAVGTTTLVAHATGARDRARANLVFNQAQVLSILVGV
                     FFLAGAMTLRDLYATALSADEKTRALANDYLLWFIPAMALQFGLIAMGAALRGTGNFR
                     PGMIVQTGTVIINIVLAPVLVFGWGTGRPLGVAGAALATFIAVAIGVVWMTLYFAPQD
                     SYLKFSPREWAPRWSLWGDLTKIGLPAGAEFGLLAAYMILVYAVSRPFGAAAQAGFGI
                     GMRILQACFLPVVALGFAVSPVAGQNFGARQPARVRATFKIGASLAAAAMLLLALAVA
                     FWADAMVRVFTSDPRAVGTGEEYLHILAWSFVASGVIFVSSSMFQAMGNTVPSLVTSA
                     LRIVLVAVPVLVLAGTPGFSLRWIWSISASAVIVQLASSLLLLRREFRLRLNFAKEES
                     GLVLRSLDAET"
     gene            2468..2719
                     /locus_tag="AC16CY_18020"
                     /pseudo
     CDS             2468..2719
                     /locus_tag="AC16CY_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709890.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
BASE COUNT          426 a          969 c          953 g          546 t
ORIGIN      
        1 tcatctgcca cgggaacaac cgcttctgta cctccgagaa cggcgtgcgc atgttgccga
       61 cgccgagcag ccgcaggtcc atcatcacga tcagtcccgc gaagatgcac atgctgatca
      121 cgtgcgagac cgtcaggtac ggcgagacgt actgcgactc gcggatcgcg atgctcgtgg
      181 ggaactcgct cacccagaag aacaggtcgt gcagcggctt catcagatcg gccatgtgcg
      241 ccttacgcct cttgccctgc gcccagtgcg tggatcaggg catgcagcgg tcatcgggcg
      301 ccttcggcgt gtctcgatac ttctcgtgac acgcgagaca ggcgtcggac accgtcccgc
      361 tgacctcgat catctggtcg atgttcttgg ccttggccgc tttgagtgtg gccgctccgg
      421 catcggcgag cccctgcgcc cacttctgga agtcggcctg atcgacgggc accggcttcc
      481 cgttctggca catgcgtccc ggaatcagaa tgaggttcgc cgtctcctgc agggcgatag
      541 cggcgttctc gaccgccacc cagccgccgt acacgccgga gtactgctgt gtcgccgctg
      601 cacctgctcc cccggacttc tgcggagccc ccggatcgtt ggtctgcgtg tcgaagatga
      661 tgttggagtt cgggaacgga attccctgca tcacctgcgc gagcgtgccg tacaccttgg
      721 gctgcggtgc cgccggagcg ggagcgggcg ccggcgccgg cgcctgggtg cacgagatga
      781 acagagccgt gatcgcgacg agcccgtaca cgcgcttcat aagcgtcctc caacacgacg
      841 gccgcagact gcctggcggc ccgaaacgga ttcggcacac gttaccttga cgatctgcac
      901 gctttcaacc tggaagtttt cctggtgggg aaacctgcgc gcggaaggtg gcgggggcgc
      961 ctggtgcaca atcggcgaac gagtctatct cacggttttc cagggggtca aggtaagcta
     1021 agcatctcgg aggccccacg tgcaggatct caccaccggt tctctcaccg gccatctcct
     1081 gaagacgacc agctacatgc tcgtcagcat gctgttccag acgctgtacg tgctcgtgga
     1141 tctctattgg gtcggccggt tgggcaccga ggccgttgcg gcggtcgccg tcagcggcaa
     1201 cctgacgttc gtcgtgctgg cagcgacgca gttgctggcg gtcggaacca cgacgctcgt
     1261 cgcgcacgcg accggagcga gggatcgggc ccgggccaac ctggtgttca accaggcgca
     1321 ggtgctttcg atcctcgtcg gcgttttctt cctcgccggc gcgatgacct tgcgcgacct
     1381 gtacgccacg gctctcagtg ccgacgagaa gacccgggca ctggccaacg actacctgct
     1441 ctggttcatt ccggcgatgg cgctgcagtt cggcctgatc gccatgggcg ccgcccttcg
     1501 aggcaccggg aacttccggc cgggcatgat cgtgcaaacc ggcacggtca tcatcaacat
     1561 cgtcctcgcg ccggtgctcg tcttcggatg gggcacgggt cggccgctcg gcgttgccgg
     1621 ggcggccctg gccacgttca tcgcggtggc catcggcgtg gtctggatga cgctgtactt
     1681 cgcgccgcag gactcgtact tgaagttctc gccgcgggag tgggcgccgc gctggtcgct
     1741 ctggggcgac ctgacgaaga tcggcctccc ggccggcgcc gagttcggtc tgctcgcggc
     1801 ctacatgatt ctcgtctacg ccgtgagccg ccccttcggc gcggcggcac aggccggctt
     1861 cgggatcggg atgcgcatcc tgcaggcgtg ttttcttccg gtcgtggcac tgggcttcgc
     1921 ggtgagcccg gtcgccggtc agaacttcgg cgccaggcag ccggcgcgcg tccgggcgac
     1981 gttcaagatc ggcgcgtcgc tggccgcggc ggcgatgctc ctgctggccc tcgccgtcgc
     2041 gttctgggcc gacgccatgg tgcgggtctt cacgtcggat ccgcgggcgg tgggcactgg
     2101 cgaagagtac ctgcacattc tggcctggag ctttgtcgcc tcgggagtga tcttcgtcag
     2161 ttcgagcatg ttccaggcca tgggcaacac ggttccctcg ctggtcacgt cggccctccg
     2221 gatcgtgctg gtcgcggtgc cggtgctggt cctcgccggc acacccgggt tttcgcttcg
     2281 ctggatctgg tccatctccg cctccgcggt catcgtccaa ctcgcctcga gcctcctgct
     2341 gctgcgccgg gaattccgcc tgcggctcaa tttcgccaaa gaagagagcg gcctggtgct
     2401 tcggagcctc gacgcggaga cctgacctcg tgcttcgcgc cgccgtttcg ttataagctt
     2461 ctctgccatg ccgttcatca tcaccgaccc ctgcattggc acgaaggatt ccgcctgcgt
     2521 cgacgtctgt ccggttgact gcattcaccc gaggaaggac gagccggagt tcgcgacggc
     2581 gacgatgctc tacatccatc cggacgagtg catcgactgc ggcgcctgcg tgccggcctg
     2641 ccccgtggcc gccatctacg acagcgttga ctccacgccg ccgtcccaga aggacctcat
     2701 cgaggcgaac gcggtatacc gcgcgggcga tgcggcggcc gtcgcgaatg ccgaagcgat
     2761 ggtccaggct cacatcgcgt cgcacgccga cctgatggcc gtggccccca cggagcgcgc
     2821 cgcggcgcac gccaagtaat ggcgcggggc tgggagagca aatccgtcga ggcgcagcag
     2881 gaggaggcgg cggc
//
LOCUS       DBNGSH010000784         3538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3991919, whole genome shotgun sequence.
ACCESSION   DBNGSH010000784 DBNGSH010000000
VERSION     DBNGSH010000784.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3991919"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="AC16CY_18025"
     CDS             <1..299
                     /locus_tag="AC16CY_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355754.1"
                     /translation="CLDAKTGKTVYGPQRLRNATYSGSAVLADGKIYITDEDGVTTVL
                     KAGPTFEVLAENDLADYTLSSPAVSEGQIFIRTASFVYAIGQRTAGRQLPVGSK"
     gene            378..1841
                     /locus_tag="AC16CY_18030"
     CDS             378..1841
                     /locus_tag="AC16CY_18030"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3355755.1"
                     /translation="MIRRVLKAVVVAIAAVVIVGGVLYQFFGLRFVMDGGGIPRPQFV
                     ETRDRQAERIEEHRAAQRAAAPAVESIGAATPASEPATAAPGVDVHGAAAAAASAPAP
                     YWTDFRGPLRDGDYREVPILQEWPAGGLKPMWKQPAGGGYASFVVARGRAFTIEQRGG
                     QEVAAAYDVATGRELWTNAWAAEFRESMGGDGPRATPTWSDGRVYALGATGELRALDE
                     RSGRVIWRTNILSDSGADNLPWGMAASPLVVDETIVVLPGGPNGQSVAGYDRATGKRL
                     WSALDDMQAYCSPMLVTLNGVRQLLVFSATRLTGLSPSNGQLLWSFPFPTYNGINSAQ
                     PIVIGDNRVFISASYGAGAVMLELSRDADGFAVREVWRNNRMKNRFSTSVLRDGVIYG
                     LDESILAAIDAGTGELKWKGGRYGYGQLLLAGNNLIVLTEDGDLALVRATPERHQELV
                     RFPVLDGKTWNVPALAGGYLLVRNLAEMAAFDLRVSR"
     gene            1877..3139
                     /locus_tag="AC16CY_18035"
     CDS             1877..3139
                     /locus_tag="AC16CY_18035"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3355756.1"
                     /translation="MAAALVSVVPSAPRVEGQQPPPAVQMIGDTGEAAKYWARWRGPS
                     GQGLVSGTGYTDTWSATQNVKWKIPVPGSGNSSPIVWGDRIFLTTAYDGGRRLSVLAY
                     RRADGARMWETFAPDGRTGFGSHFKNGHASATAATDGQRVYVSFGARGILALDMNGKL
                     VWHRDLGPMNVYHGTAGSPLLYKDRLIFYQDQAEGSFIAAFDTRTGKTLWTTRRTASV
                     GWGTPIAIRVVDHDEIIVNGQLKVVAYNPDTGAEIWSCDGSTFEVIPTPVVGYGMVFC
                     SSGRAGPTLAIRPGGKGDVTRSHLEWTTPRGSPFVPSPILYGEHLYMVNDMQSVATSF
                     DARTGKVMWQNRLGVARREGFSASPVAFDGKVFFTNDEGETFVLKAGATFQLLHVNNI
                     GEATLATPALVDGLWYIRTAQSLFAIGK"
     gene            complement(3140..>3538)
                     /locus_tag="AC16CY_18040"
     CDS             complement(3140..>3538)
                     /locus_tag="AC16CY_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355757.1"
                     /translation="GALLHLGQSQLGNPLKALLCVGAAYFLIGVLIPVAALGSQGQLS
                     AFNTSGLVTATIAGALGAAGAACIIWAFRTGGLPVYVMPLVFGGAPIVNVAVSMLIHP
                     PKSALSPMLFVGFVLASVGAGMVLYFRPTS"
BASE COUNT          597 a         1140 c         1253 g          548 t
ORIGIN      
        1 actgcctcga cgccaagacg gggaagacgg tgtacggacc gcagcggctg cggaatgcca
       61 cctacagcgg ctcggccgtg ctggccgacg ggaagatcta catcaccgac gaggacggcg
      121 tgaccacggt gctcaaagcc ggccccacgt tcgaagtcct ggcggagaac gatctcgccg
      181 actacacgtt gagctctccc gctgtgtcgg aagggcagat cttcatcaga accgcgtcgt
      241 tcgtgtacgc gatcgggcag cggacggccg gtcggcagtt accagtgggc agcaagtagc
      301 ggcacacgtc gtaggggccg gtctttagac cggcccaggc gggtctgaag acccgcctct
      361 acgcggaggg ggggctggtg attcgcaggg tcttgaaggc cgtcgtcgtt gcaatcgccg
      421 ctgtcgtgat tgtcgggggc gtcctctacc agttctttgg cctgcggttt gtcatggacg
      481 gcggcggaat cccgcggccg cagttcgtcg agacacgcga tcgccaggcc gaacggatag
      541 aagagcatcg cgcggcccag cgagccgcgg ctccagcggt cgagtccatt ggcgccgcga
      601 cgccggcttc tgaaccggcc accgccgcac cgggtgtgga tgttcacggt gcggccgccg
      661 cggcggcgtc cgctcctgcc ccctactgga ccgactttcg cggcccgctc cgcgacggcg
      721 actaccgcga ggtgccgatc ctgcaggaat ggcccgcggg cggcctgaaa ccgatgtgga
      781 agcagcccgc cggcggcggg tacgcgtcgt tcgtggtcgc gcgcggccgg gcgttcacga
      841 tcgaacagcg cgggggccag gaagtcgctg ccgcctatga cgtggccacg ggccgcgagc
      901 tgtggaccaa cgcgtgggcc gccgagttcc gcgaatcaat gggcggcgac ggccccaggg
      961 caacgcccac gtggtcggac ggccgcgtgt acgcgctcgg cgcgaccggc gagctccgcg
     1021 ccctggacga acgcagcggc cgcgtgatct ggcgaaccaa catcctcagc gacagcggcg
     1081 cggacaacct gccctgggga atggccgcgt cacccctcgt cgtggacgag acgattgtcg
     1141 tgctgccggg tggtccgaac ggacagtccg tggccggcta cgaccgcgcg accggcaagc
     1201 ggctctggtc ggcgctcgac gacatgcagg cgtactgctc gccgatgctc gtcacgctga
     1261 acggcgtgcg gcagctcctc gtgttcagcg cgacgaggct gacggggctg tcaccctcga
     1321 acggccagct gctgtggtcg ttcccgtttc cgacctacaa cggcatcaac tcggcccagc
     1381 cgatcgtgat tggcgacaat cgtgtgttca tctcggcgag ctacggcgcc ggtgccgtca
     1441 tgctggagct gtcacgagac gctgacggtt tcgccgttcg cgaagtctgg cgcaacaacc
     1501 ggatgaaaaa caggttctcg acgtccgtgc tgcgggacgg cgtgatctac gggctcgacg
     1561 agtcgattct cgcggcgatc gacgccggga cgggtgagct caagtggaag ggcgggcgct
     1621 acggctacgg ccagctgctg ctggccggca acaacctgat cgtactgacc gaagacggcg
     1681 atctcgccct cgttcgcgcg acgccggagc ggcaccagga gctcgtgcgt ttcccggtgc
     1741 tcgacggcaa gacgtggaac gtcccggcgc tggcaggagg ctacctgctg gtccggaacc
     1801 tggcggagat ggcagctttc gacctgcggg tgtccagatg aggaatcgag tcctttcaat
     1861 ggttcgggga gcgcttgtgg cggccgcact cgtgtcggtg gtcccatcgg ctccgcgggt
     1921 cgaagggcag cagcctccgc ccgccgttca gatgatcgga gacaccggcg aagcggcgaa
     1981 gtactgggcg cgctggcgcg gtccatcggg acaagggctc gtgagcggga cggggtacac
     2041 cgacacctgg tcggcgaccc agaacgtgaa gtggaagatt cccgtaccgg gaagcggcaa
     2101 ttcgtcgccg attgtctggg gcgatcgcat attcctcacg accgcctacg acggcggccg
     2161 gcgtctgtcg gtcctggcgt accgccgggc ggacggggcg cgcatgtggg agacattcgc
     2221 gccggacggg cgtacggggt tcggcagcca cttcaagaac gggcacgcct cggccacggc
     2281 agccaccgat ggccagcgcg tctacgtgtc gttcggcgcc cgcggcatcc tcgcgctcga
     2341 catgaacggc aagctggtct ggcatcgcga cctcgggccg atgaacgtgt atcacggcac
     2401 ggccggctcg ccgctgctct acaaggatcg gctcatcttc taccaggacc aggctgaagg
     2461 ctcgttcatt gcggccttcg acacgcgcac ggggaagaca ctgtggacga cgcggcggac
     2521 ggcgagcgtg ggctggggca cgccgatcgc tatccgcgtc gtcgatcacg acgagatcat
     2581 cgtcaacggc cagctcaagg tggtggcgta taacccggat acgggcgccg agatctggag
     2641 ctgcgacggc tcgaccttcg aggtcatccc gacgccggtc gtcggctacg gcatggtgtt
     2701 ttgttcgtcc gggcgcgccg gcccgacgct ggccattcgc ccgggcggca agggggacgt
     2761 gacccggagc catctggagt ggacgacccc gaggggctcc ccgtttgttc cgtcaccgat
     2821 tctgtatggc gaacacctgt acatggtgaa cgacatgcag agcgtcgcga cgagcttcga
     2881 cgcgcgcacg ggaaaggtga tgtggcagaa ccgcctcggc gtggcgcggc gggaaggatt
     2941 ctcggcgtcg ccggtcgcgt ttgacggcaa ggtgtttttc acgaacgacg aaggcgagac
     3001 gttcgtgctg aaggccgggg cgacgtttca gctgctccac gtgaacaaca tcggcgaggc
     3061 gacgctcgcg acgccggccc tggtggacgg cctgtggtac atccgcaccg cgcagagcct
     3121 ttttgcgatc gggaagtgat caggacgtcg gcctgaagta gagcaccatc cccgcgccga
     3181 ccgacgccag cacgaacccc acgaagagca tggggctgag cgcggatttc ggcggatgga
     3241 tcagcatcga gaccgccacg ttgacaatcg gtgcaccgcc gaagacgagc ggcatcacat
     3301 acaccgggag cccgcccgtc ctgaacgccc agatgatgca cgcggcgccg gccgcgccga
     3361 gggcgcccgc aatggtcgcc gtgacgaggc ccgaggtgtt gaacgccgac agctggccct
     3421 gcgagccgag ggcggccacc gggatcagca cgccgatcag gaagtaggcc gcgccgacac
     3481 acagcaacgc cttgagcgga ttaccgagct gcgactgccc gagatgcagc agcgcccc
//
LOCUS       DBNGSH010000785         2422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4162646, whole genome shotgun sequence.
ACCESSION   DBNGSH010000785 DBNGSH010000000
VERSION     DBNGSH010000785.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4162646"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="AC16CY_18045"
     CDS             complement(<1..1180)
                     /locus_tag="AC16CY_18045"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3355758.1"
                     /translation="MSSVWRPLVLATVTVLVLPVAVNVQSGDGPRPPAGKEWPAVGGD
                     SGNSRHSTLNQITTQNVTKLGGAWTPQKLDSSPATRSMPVVRDGLMFLTAGPNVYALD
                     ARTGARVWRFQGGQQAGMGAPDREGVAVGDGLVFVGLSDARVIALREKTGELVWNKYV
                     GDKARDKGQVISGAPLYAGGLVSVGLSADNGWRGQIVALNGKTGDEAWRFFAVPAPGE
                     PGSETWPKGSPIWQRGGGAVWLVGAADPDLGLVFYVTGNGVPQLAGEGRPGANLYLCA
                     VLAIDMKTGKLRWHYQTIRHDIWEADIAISPVLYDAQIEGRSRKAVAAMRADGYLFML
                     DRETGKPLSRIEDRPVPQDARRTTAATQPFPAGADSALPDCAHWKQQPLPQGFDLGCD
                     F"
     gene            1586..2152
                     /locus_tag="AC16CY_18050"
     CDS             1586..2152
                     /locus_tag="AC16CY_18050"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3355759.1"
                     /translation="MTRTSPGVKLTYDDFVQFPDDGLRHELIDGEHYVTPSPNTKHQR
                     VSLNLILLIGGWLEHNPIGQLFHAPFDVVFSKFDVVEPDLLYLSNARAAESLTPQHVR
                     GVPELVIEIGSPGTRQRDETVKRRLYERSGVSEYWVADPEIDTIRVYRREGDQFARVV
                     ELSADAGDTLTTLLLPGLEVPLLKVFRA"
     gene            2225..>2422
                     /locus_tag="AC16CY_18055"
     CDS             2225..>2422
                     /locus_tag="AC16CY_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355760.1"
                     /translation="MKRAAAGAAAIAGSGIVAGPAFAGAAAQNASPADLALVNGKFVD
                     GRGQVATGLTIRNGRIAGIGEA"
BASE COUNT          404 a          820 c          787 g          411 t
ORIGIN      
        1 agaagtcgca tccgagatcg aaaccctgcg gcagcggctg ttgtttccaa tgggcacagt
       61 cgggcaacgc gctgtcggcg cccgcaggaa acggttgcgt cgccgcggtg gttcgacgcg
      121 cgtcctgcgg cacgggccgg tcctcgatgc gggacagcgg ctttcctgtt tcgcggtcga
      181 gcatgaacag atagccgtcg gcacgcatcg cggcaaccgc cttacgcgaa cgtccctcga
      241 tctgcgcgtc gtacaggacg ggcgaaatgg cgatgtccgc ttcccagatg tcgtggcgaa
      301 tcgtctgata gtgccagcgg agcttcccgg tcttcatgtc gatggccagc acggcgcaga
      361 gatacaggtt cgcgccagga cgtccttcgc ctgcgagctg cggtacgccg ttgccggtga
      421 catagaacac cagtccgaga tccggatcgg ccgcgccgac gagccagaca gcgccgccgc
      481 cccgctgcca gatcgggctg ccctttggcc aggtctccga tccgggctcg cccggcgcgg
      541 gcacggcgaa gaagcgccac gcttcatcgc cggtcttgcc gttcagcgca acaatctgac
      601 ctcgccagcc gttgtcggca ctcaaaccga cggagacgag tccgcccgcg tacaggggag
      661 ctcccgagat cacctgtccc ttgtcgcgcg ccttgtcgcc gacgtacttg ttccagacga
      721 gctcgcccgt cttttcacgc agcgcgatga cgcgcgcgtc cgagagcccc acaaagacga
      781 ggccgtcgcc gaccgccacg ccttcgcggt ccggagcgcc catgcccgcc tgctggcctc
      841 cctgaaatcg ccagactcgg gcgcccgtcc gggcatcgag cgcatagacg ttcggccccg
      901 ccgtcaggaa cattagacca tcgcggacga cgggcatcga gcgggtggcg gggctggagt
      961 cgagcttctg cggcgtccac gcgccgccaa gcttcgtgac gttctgcgtg gtgatctggt
     1021 tgagcgtcga gtggcggctg ttacccgagt cgccgccgac ggccggccac tccttgccgg
     1081 cagggggccg tgggccgtcg cccgactgca cattgacagc aaccggcagg acgaggaccg
     1141 tcaccgtcgc gagaacgagg gggcgccaga cgctgctcat ggggaatgct ccttcgggag
     1201 cagatgatac tcctcgacgt ctgtcacggg tccggctaaa gccggacgct acgtacgagc
     1261 aggcgtagtg tccggcctag ccggacctcc tcctataacg ggcaccggcg ccgctcacgc
     1321 tgctggttcg ccgctggtag cccagtacat gccgctctcg tagtccatcg cacaatcggt
     1381 gtccgccgga gaagtcgcca gtggcgtgtg gcaggaacgc ggcgtcccag cgccgcacga
     1441 cctcgcagaa actgcgtacc aaggccggcg ctcggacgca ctgcttcacg atcagcccag
     1501 tcaatcggcc cctcgctgac agcgcttcca atggcatggt tcgagcacat gggcctagaa
     1561 tgagcccatg gccgggagcg accgtgtgac tcgcacttcc ccgggcgtga agctcaccta
     1621 cgacgacttc gtgcagttcc ccgacgatgg gctgcggcac gagttgatcg acggggagca
     1681 ctatgtgacg ccttcgccca ataccaaaca tcagcgcgtg tcgctcaacc tgatcctgct
     1741 catcgggggc tggctcgaac acaacccgat cggccaactc tttcatgcgc cctttgacgt
     1801 ggtgttctcc aagttcgacg tggtggagcc ggatttgctc tacctctcga acgcacgagc
     1861 agccgagtcg ctgacgccgc agcacgtccg aggcgtgcct gagctcgtca tcgagatcgg
     1921 gtcgcccggc actcgacagc gggatgagac agtgaagcga cgactgtacg aacggtcggg
     1981 agtctcggag tactgggtcg ccgatcccga gatcgacacc atccgcgtct atcgtcggga
     2041 gggcgaccag tttgctcgcg tcgtggagct ttccgccgac gccggggata cccttaccac
     2101 gctgctcctg ccaggcctcg aggtcccgct cttgaaggtc ttccgcgcgt aagcggacgt
     2161 tgccttgtta ggtgtactca tgccagaaat cggtttaccc tgcaccctgt cgcggcgtga
     2221 gctgatgaag cgcgcggcgg ccggcgccgc agccattgcc ggcagcggga tcgtggccgg
     2281 acctgccttc gcgggagcgg cggcgcagaa tgccagcccc gccgatctcg ccctcgtgaa
     2341 cggcaagttc gtggacggcc gcggccaggt cgcgacgggg ctgacgatca ggaacggccg
     2401 catcgccggc atcggcgagg ct
//
LOCUS       DBNGSH010000786         3048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2972850, whole genome shotgun sequence.
ACCESSION   DBNGSH010000786 DBNGSH010000000
VERSION     DBNGSH010000786.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2972850"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="AC16CY_18060"
     CDS             complement(<1..404)
                     /locus_tag="AC16CY_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HIR3355761.1"
                     /translation="MQDTGFFGHPRGLSTLFFTEMWERFSYYGMRAFLIFYMVASPAS
                     GGLGFSDADAASIYGTYTASAWGLAIFGGIVADRFLGQYRSVLIGGIIIALGHFTLAF
                     PPVPFFYAGLALVAIGTGLLKPNVSNLVGSLY"
     gene            437..1822
                     /locus_tag="AC16CY_18065"
     CDS             437..1822
                     /locus_tag="AC16CY_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_334096974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM (seleno)protein"
                     /protein_id="HIR3355762.1"
                     /translation="MRVLLISTYDLGRQPAGLASPAAWLRGAGVDVECVDVSRDPLHD
                     GQIGAADLVAFYLPMHTATRLAAPLVSRVQRVNARARLAAYGLYAPLNAEWLREHGVD
                     HVLGPDAEADLVTLATDGQEPRAESQGPRARLHFVRPDRSTLPPLSRYAALQMPDGSR
                     RVAGATDATRGCKHTCRHCPIVPVYNGTFRAIPIDIVIEDIRAQAAGGAQHISFGDPD
                     FLNGPTHARRLVERLAREFPGLTYDATIKIEHILQHAAMLPLLRNTGCLMVTSAVESI
                     DDGVLGKLEKGHTRADFVEAVGLCRGAGIALAPTFIPFTPWTTIEGYVELLDEIDRLD
                     LTQDVAPIQLAIRLLVTARSRLLELPDINAIVEPFDAASLTFPWRHPDPRVDALQREV
                     MQIVSTNAHASRAEVFDRIVAGVKGSDPFTLLHDPGCGESCRARGPTPPLMTEAWYCC
                     AEPGPELLDLV"
     gene            1819..>3048
                     /locus_tag="AC16CY_18070"
     CDS             1819..>3048
                     /locus_tag="AC16CY_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3355763.1"
                     /translation="MTRVLLLSTTTGYQIRSFGEAAGALGVRLVIGSDRCDRLEDPWW
                     DGAIPMRFHDTDASVDAVTTAWQGPPPSGVIAVGDRPTVLAARLAAAFGLPGNPVDAA
                     EASRDKQASRRAFAAAGLLTPPWQVFPTTQDPSAVRAIEYPAVIKPLALSGSRGVIRV
                     DGEDALVAAFHRIRHLLRAPDVVIQRDGLHDFVLVESYVPGREYAIEGVLSGGTLTVF
                     AIFDKPDPLDGPFFEETIYVTPSRAATGVQRAMIDAVAAAARALGLTHGPVHAECRVN
                     ERGVYVLEVAARPIGGLCSRAVRLEGPGRIAMTLEDVLLRHAIGERIDQCRPAARASG
                     VMMIPIPRRGVFRGVTGVDRARDVPGVEDVRITAKPDALLVPLPEGRSYLGFIFVSAE
                     TPARVEEALREAHAQLNF"
BASE COUNT          479 a         1018 c         1052 g          499 t
ORIGIN      
        1 tcgtagagag acccgacgag gttgctgacg ttcggcttca gcaggccggt gccgatcgcc
       61 acgagtgcga ggccggcata gaagaacggc acgggtggaa acgccagcgt gaagtgcccg
      121 agcgcgatga tgatgccgcc tatcagcacg ctgcggtact ggcccaggaa tctgtcggcg
      181 acgatgccgc cgaagatcgc gaggccccac gcgctggcgg tgtaggtgcc gtagatcgag
      241 gcggcgtcag cgtcggagaa gccgagcccg ccggatgccg gcgaggccac catgtagaag
      301 atgagaaagg cccgcatgcc gtagtagctg aagcgctccc acatctccgt gaagaagagc
      361 gtggacagcc cgcgggggtg gccgaagaat ccggtgtcct gcatcgccgg caaggataac
      421 gtggaacaat gaggcggtgc gcgtcctcct gatttcgacc tacgacctcg ggcgccagcc
      481 cgccggcctc gcgtcgccgg ctgcgtggct ccgcggcgcc ggggtcgatg tggaatgcgt
      541 ggacgtgtcg cgcgatccgc tgcacgacgg ccagatcggg gccgccgacc tggtggcgtt
      601 ctacctcccc atgcacacgg ccacgcgcct ggccgcgccg ctcgtgagcc gggtgcagcg
      661 cgtcaacgcc cgcgcgcggc tcgcggccta cgggttgtat gcgcccctca atgcggaatg
      721 gctccgggaa cacggggtcg atcacgtgct cggtccagac gccgaggcgg atctggtcac
      781 gctcgcgacc gacgggcagg agccgagggc cgagagtcaa gggcccaggg cccgtcttca
      841 cttcgtccgg cccgatcggt cgacgcttcc gccgctgtcc aggtatgccg cgctgcagat
      901 gcccgatggc agccggcgcg ttgccggggc caccgacgcg acgcgcggtt gcaagcacac
      961 gtgccggcat tgtccgatcg tgcccgtcta caacggcacg tttcgggcga tccccatcga
     1021 catcgtgatc gaggacatcc gcgcgcaggc ggccggcggc gcgcagcaca tctcgttcgg
     1081 ggatcccgac ttcctgaacg gcccgacgca cgcgcgccgt ctggtcgagc ggctcgcgcg
     1141 cgagtttccg ggcctcacct acgacgccac gatcaagatc gagcacatcc tccaacacgc
     1201 cgccatgctc ccgctcctgc gcaacacggg gtgcctcatg gtgacgagcg ccgtcgagtc
     1261 gatcgacgac ggcgtgctgg gcaagctcga gaaggggcac acgcgcgcgg atttcgtcga
     1321 ggcggtcggc ctctgcagag gcgcgggaat tgctttggcg ccaacgttca ttcccttcac
     1381 cccgtggacg acgatcgagg gatatgtcga actgctcgac gaaatcgatc gactggacct
     1441 gacgcaggac gtggcaccga ttcagctggc gatccgtctg ctcgtgaccg ccaggtcgag
     1501 gctgctggag ttgcccgata tcaacgcgat cgtggaaccg ttcgatgccg cgtcgctgac
     1561 gttcccctgg cggcatccgg atccccgcgt cgatgccctt cagcgggagg tgatgcagat
     1621 cgtgagcacg aacgcccacg cctcccgcgc ggaggtcttc gatcgcatcg tggcaggtgt
     1681 gaaggggtca gaccccttca cactcttgca cgacccggga tgcggcgagt cctgccgcgc
     1741 ccgtggtccg accccaccgt tgatgaccga ggcgtggtac tgctgcgcgg aacccggtcc
     1801 cgagctcctc gacctggtgt gacacgagtc cttcttctgt ccacgaccac gggctaccag
     1861 atccgctcct tcggcgaagc ggccggcgct cttggcgtgc ggctcgtgat tggcagcgac
     1921 cgctgcgacc gcctcgagga tccctggtgg gatggcgcca tccctatgag gtttcacgac
     1981 acggacgcgt cggtggatgc tgtcaccacg gcatggcagg ggccgccgcc gtcaggagtg
     2041 atagccgtcg gcgaccgtcc aaccgtgctg gcggcccgcc tggccgcggc attcggattg
     2101 ccggggaatc ccgtggacgc agccgaggcg agccgcgaca agcaagcctc gcgtcgcgcg
     2161 ttcgccgcgg ccggtttgct cacgccaccc tggcaggtat ttcccacgac acaggatccg
     2221 tcggccgtgc gtgcgatcga gtatccggcg gtcatcaaac cgctggcgct ctccggaagc
     2281 cgcggcgtga tccgcgtaga cggcgaggac gccctcgtcg cggccttcca ccggatacgc
     2341 catctgcttc gtgcgccgga cgtcgtgatc cagcgagacg gcctgcacga tttcgtgctc
     2401 gtcgagtcgt acgttccggg ccgcgagtac gcaatcgagg gcgtgttgag cggcgggacg
     2461 ctgacggtgt tcgcaatctt cgacaagccg gatccgctcg acggtccgtt cttcgaggag
     2521 acgatctacg tcactccctc acgcgccgcg accggcgtgc agcgggccat gatcgacgcg
     2581 gtcgccgcgg ccgcgcgtgc cctgggcctc acgcatgggc cggtgcacgc ggaatgtcgt
     2641 gtcaacgagc gcggcgtgta tgtgctcgaa gtggctgccc gccccattgg cggactctgc
     2701 tcgagggccg tgcgcctgga aggcccgggc cggatcgcga tgacgctcga ggacgtgctg
     2761 ttgcgccacg cgatcggcga gcgaatcgat cagtgtcggc cggcggcacg cgcatcaggc
     2821 gtgatgatga ttccgatccc gcgtcgtggc gtcttccgcg gtgtcaccgg tgtcgatcgc
     2881 gctcgagacg ttccaggcgt cgaggacgtg aggattactg cgaagccgga cgcgcttctc
     2941 gtgccgctgc cagaagggcg cagctacctg ggcttcattt tcgtaagcgc ggagactccg
     3001 gcgcgcgtcg aggaggcgct gcgcgaggcg cacgcgcaac tcaatttc
//
LOCUS       DBNGSH010000787        11549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2973080, whole genome shotgun sequence.
ACCESSION   DBNGSH010000787 DBNGSH010000000
VERSION     DBNGSH010000787.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11549
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2973080"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="AC16CY_18075"
     CDS             <1..1532
                     /locus_tag="AC16CY_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3355764.1"
                     /translation="DRVALFMPMCPELVAAFFAVIKAGGIVLPLFSGYGADAVATRLQ
                     DAGVRLLVTADGFWRRGAAVKTKETADAAVRAASTVDRVVVVSRLGLDVQMGPRDVWW
                     SHLLSDQPETFETERTGADDPLMIIYTSGTTGRPKGAVHTHCGFPVKAAQDMVHAFDV
                     HAGETMYWVSDMGWMMGPWEVLGMTLIGGTIVLYDGALDFPSPDRLWAIVERHRVNVL
                     GVSPTLIRALMKHGEGPVRAHDVSSLRILGSTGEPWNPEPWRWFFDVVGGGRLPVINY
                     SGGTEVSGGLVAGNVLTPLKPAAFAGPPPGIAADVVDDQGRPVRNQVGELIVRAPWIG
                     MTRGFWNDDERYMQTYWSRFPDVWLHGDWAAIDEDGLWYILGRSDDTIKIAGKRLGPA
                     EVESVLVDDPSVLEAAAVGVPDELKGQALVCFCVLRPGHEAGPALRSRLSALVVDRLG
                     KPLQPRAIEFVGDLPKTRNAKVMRRVIRAAYIGEAPGDLSSLENPQAIDEIRATRAIR
                     N"
     gene            complement(1522..2274)
                     /locus_tag="AC16CY_18080"
     CDS             complement(1522..2274)
                     /locus_tag="AC16CY_18080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HIR3355765.1"
                     /translation="MSDVARAAVYYENCLGFHWDWGAEGLGQVSRGDCRIFLTDNAFR
                     GDDSTGTPMVIWINLDSKADVDALYESWSGRGAQIVSKPESQPWHLHEFTARDLDGNR
                     FRVFYDFAWELPDRGGRKDDAAERAAARRSTPDVVGGTSDVRIFHITSEQAWTDAQAQ
                     GTYGADSLASEGFIHCSEPHQVAWVANQRFRGRSDLVLLHIDPARLQAPVKYENLEGG
                     RELFPHVYGAVPVSAVVSVTRLLPSDSGDFSF"
     gene            complement(2319..3182)
                     /locus_tag="AC16CY_18085"
     CDS             complement(2319..3182)
                     /locus_tag="AC16CY_18085"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR3355766.1"
                     /translation="MSDDPPFQNIAIVGLGLIGGSIALAVRERWPSVRVAAMDRGPVL
                     AHAMGSGAIDRTVQDLSDLKGSDLLVLAAPVDQNVRLLPEAARCLGPDAVITDVGGTK
                     RDILEAAAALSVPHPTFVGGHPVGGAERGGFSFARADMFRGRPWVFTPGSPAPTQAVD
                     RLCDFARGLGARPTTMEAGEHDRLMAFLSHLPQLTASVLMEIVGNAVTASGLKLAGRG
                     LVDTTRLASSSPDIWRGVCASNADVIGVALDALIDRLEQLRGGLQQEGVVEAVFTAAA
                     RWRAELMKGDG"
     gene            complement(3270..4316)
                     /gene="aroF"
                     /locus_tag="AC16CY_18090"
     CDS             complement(3270..4316)
                     /gene="aroF"
                     /locus_tag="AC16CY_18090"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3355767.1"
                     /translation="MVVVMKERATEGQIDAVIARLIELGMDVHRSSGATRTVLGVVGA
                     HKVDPELIGILDGVHEVLRISEPYKRASRSFKPEDTVVALEDVRVGGDEVIVMAGPCS
                     AETEEQVMASAAAVKRAGAKILRGGAFKPRSSPYSFQGLGEEGLQMLRRAADAHNLKL
                     ISEVMDISQIELVGRYSDIFQVGARNMQNYTLLRELGHVRKPVLLKRGISATIEEWLL
                     SAEYILAGGNNDVILCERGIRTFETATRNTFDISAIPVVKKLSHLPIVADPSHGAGRR
                     DMVAQLARAAVAAGADGLIIEVHNDPDRALSDGAQSMFPAQFDRLMAELRIIAPAIGR
                     SICLEPVARRGWAR"
     gene            complement(4319..4639)
                     /locus_tag="AC16CY_18095"
     CDS             complement(4319..4639)
                     /locus_tag="AC16CY_18095"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR3355768.1"
                     /translation="MAEVLTIEELRNRIDVIDEQLVRLLNVRVACAVEVGRLKHEAAL
                     PIYQPEREAEVLNKVRRSATDLAGPLTAEAVVRIFERVIDEARRAERVASDRRDGSRS
                     GMGE"
     gene            complement(4624..5409)
                     /gene="trpA"
                     /locus_tag="AC16CY_18100"
     CDS             complement(4624..5409)
                     /gene="trpA"
                     /locus_tag="AC16CY_18100"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR3355769.1"
                     /translation="MSRLEETFARIREGGKPGLVTYITAGDPDLSKTRGVLSALDRAG
                     ADVVEIGVPFSDPLADGPVIQRATERALASGTTLTRVLDLVAEARADLRAPMVIFSYA
                     NPILRLGTERFADRAREAGVDGVLVLDLPIEEADDFRNMLRRRGIDTILLLSPTTTDD
                     RLRKAGALGSGFLYAISRLGVTGARDRIAAGAREMVERIRRVSRLPVALGFGISSPEH
                     VREVGQWADAAVVGSALVSVMAEAGATNDLNTRVEEYVRWLKS"
     gene            complement(5406..6659)
                     /gene="trpB"
                     /locus_tag="AC16CY_18105"
     CDS             complement(5406..6659)
                     /gene="trpB"
                     /locus_tag="AC16CY_18105"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR3355770.1"
                     /translation="MNPVNPANLDGPVFGARDPDARGYFGAYGGRFVPETLVAPIEDL
                     TAGYLEARADASFGDELSRLLRHFAGRPTPLYETQRLAIEAGGVVGASGASRVRILLK
                     REDLTHTGAHKINNALGQALLARRMRKTRVVAETGAGQHGVATATACALLGLECCVYM
                     GAEDMRRQALNVVRMELLGATVRRVDSGSRTLKDAINEAMRDWVSNVGNTYYLLGSAL
                     GPHPYPLMVREFQSVIGREARRQMLEQTGRLPDVVVACVGGGSNAIGIFDAFLDDRSV
                     RLIGVEAGGESISPGRHAARFAGGSTGVLQGTRTFVLQDEAGNIELTHSISAGLDYAA
                     IGPEHAWLRDLGRTEYAYATDAEALEGFQTLARLEGILPALESAHAVAYTCRLARDLP
                     DGSVVLVNLSGRGDKDVQSVAEALR"
     gene            complement(6656..7300)
                     /locus_tag="AC16CY_18110"
     CDS             complement(6656..7300)
                     /locus_tag="AC16CY_18110"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR3355771.1"
                     /translation="MTPDPIIKICGITRAADALHAVEEGATALGFVFWPRSPRVISRE
                     RARDIIATLPAHVTMVGVFVNESADGIRRVVEATGIHAVQLHGDEKPQDADGIDLPIL
                     KSVSLDDAAEQSSVWPEDTTLLLDVADPVRRGGTGVVIDWARAAEVARHHRVVLAGGL
                     TPLNVEQAIRTVSPVGVDVSSGVEESPGVKNAERVSSFLSSARRAFAALQKRTL"
     gene            complement(7297..8127)
                     /gene="trpC"
                     /locus_tag="AC16CY_18115"
     CDS             complement(7297..8127)
                     /gene="trpC"
                     /locus_tag="AC16CY_18115"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR3355772.1"
                     /translation="MTRAPDLLLAIVAATRRITDVRRERESQAALERRAAAASPRGDR
                     FVSALGRAGRTNVIAECKRRSPSRGVLAANYDPAAIARCYQAGGAVAVSVLTEPTFFD
                     GGLEHLSAVRRAVDLPLLRKDFIVDDYQLLEARASGADAVLLIVAALEQDDLVRLQVR
                     ASELGLAALVEVHDEEELTRAIDSGAQVVGVNNRNLRTLSVDVNASRRLAGRMPADVI
                     AVSESGLSTHGELRELSSQGYRAFLVGERFMTAPDPAKAITELLGGRESFSDGSVRGK
                     "
     gene            complement(8124..9146)
                     /gene="trpD"
                     /locus_tag="AC16CY_18120"
     CDS             complement(8124..9146)
                     /gene="trpD"
                     /locus_tag="AC16CY_18120"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR3355773.1"
                     /translation="MFSRLIEKLVRHEDLTTEEAAAAMREVMEGRAAPAALAGLLAAL
                     VMKGERPDEIVGLARTMREHAVKLSAPAGEVFDTCGTGGDRSGTFNVSSAAAVVVASC
                     GVRVAKHGNRSVSSQCGSADVFERLGVNIAAPPGVVERALRDASIAFFFAQTFHPSMK
                     HAAPVRRDMGVRTTFNLLGPLTNPAGASRQIVGVPRPELTQVMARALMQLGSERAWVV
                     HGAGGIDEISTTGHTKVSECRDGAVHTFFVHPTEFGIAKASPADLAGGDASTNAAIVG
                     RVLDGQRGPARDIVVLNAGAALLVAGRARSVREGIAAAEAAIDSGVARATLDRMVHAS
                     QAEAVA"
     gene            complement(9146..9712)
                     /locus_tag="AC16CY_18125"
     CDS             complement(9146..9712)
                     /locus_tag="AC16CY_18125"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR3355774.1"
                     /translation="MILVIDNYDSFTYNLVQYLGELGADVRVRRNNQVTLGEIESMAP
                     EQLLISPGPGRPEHAGITTDVIRRFGPRTPILGVCLGHQAIGLVYGGTVCRAQVPMHG
                     KTSTVVHDSKGVFAGINEPFQAGRYHSLVIGSDDVPTDLEVAARTKEDGTIMAVRHRT
                     YPVHGVQFHPESVLTEEGRKILRNFLDL"
     gene            complement(9709..11178)
                     /gene="trpE"
                     /locus_tag="AC16CY_18130"
     CDS             complement(9709..11178)
                     /gene="trpE"
                     /locus_tag="AC16CY_18130"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR3355775.1"
                     /translation="MKITSFEEFRDLAKRGTFVPVYKEIVADMLTPVSAFLKIAEHSD
                     YAFLLESVEGGEQVGRYSFLGKDPFLILRSRGGKTTVERMGETTETDSPFVATVRELM
                     AGFHSPYVPGLPRFTGGAVGYMAYDAAAWFEPVTLQAVNDADEEAGFMLFDTVLAFDH
                     VRHRILIIANARITGDDDLQSLYEFACTKIDFVERELDRTLSKAPAASAAPLEVTSNV
                     TRDEFERMVRTAKEYIAAGDIYQVVLSQRFEAAISADPFTVYRALRHVNPSPYMYFLK
                     IGGRSIVGSSPEMLVRAEGRRVETHPIAGTRPRGRNEEEDLRLAEELKRNEKERAEHV
                     MLVDLGRNDVGRVAEYGSVRVPTYMTLERYSHVMHLVSIVEGRLREEHDRLDALVACF
                     PAGTVSGAPKVRAMEIIAELENRRRGVYAGAVGYLDFAGNLDFCITIRTVVIEKGRAF
                     VQAGAGIVADSNPAAEYEETRDKARAVIRALELAQAGLS"
     gene            complement(11175..>11549)
                     /locus_tag="AC16CY_18135"
     CDS             complement(11175..>11549)
                     /locus_tag="AC16CY_18135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF036850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR3355776.1"
                     /translation="VHARRAEQSQEIAAVFGATPGPLPPAPRTWDLLVNCTPLGGPGF
                     RNVSPMPGAEFTGRLVYDLTYGPEESVLLQEARAAGCATLDGLPMLVKQAERQFAWWT
                     GQRPVPGVMEAAARRRLARRRA"
BASE COUNT         1796 a         4139 c         3730 g         1884 t
ORIGIN      
        1 gcgatcgagt ggcgctcttc atgccaatgt gcccggaact ggtggccgcc ttcttcgcgg
       61 tcatcaaggc gggcggcatc gtactgccgc tcttctccgg gtacggcgcc gacgcggtcg
      121 cgacgcgatt gcaggatgcg ggcgtcaggc ttctggtcac ggccgacggc ttctggcgtc
      181 gcggtgccgc cgtgaagacc aaggagacgg ccgatgcagc ggtccgggcc gcgtccacgg
      241 tggatcgcgt cgtcgtggtc tcgcgcctgg gactcgacgt ccagatgggc ccgcgcgacg
      301 tatggtggtc gcacctgctc tccgatcagc cagagacgtt cgagacggaa cgcaccggcg
      361 cggatgatcc gctgatgatc atctacacct cggggacgac aggtcgtccg aagggcgccg
      421 tccacaccca ctgcggcttc cccgtcaagg cggctcagga catggtgcac gcattcgacg
      481 tccacgccgg ggaaacgatg tactgggtga gcgacatggg ctggatgatg ggcccgtggg
      541 aagtgctcgg catgacgctc atcggcggaa cgatcgtcct ctacgacggc gcactcgact
      601 tcccgtcgcc cgatcgcctg tgggctatcg tcgaacgcca tcgcgtcaac gtgctgggcg
      661 tctcgccgac cctcatccgc gcgctgatga aacacgggga agggcctgtg cgcgcgcacg
      721 atgtgtcctc gctgcgcatc ctgggatcga ccggcgagcc atggaacccc gagccgtggc
      781 gctggttctt cgacgtggtc ggcggcggac ggctgccggt catcaactac tcgggcggca
      841 ccgaggtgtc gggcggcctc gtcgccggca acgtgctgac gccgctcaag cccgccgcgt
      901 tcgccggtcc cccgccgggc atcgccgccg acgtggtgga cgaccagggc cggcccgtgc
      961 gcaaccaggt cggcgagctg atcgtgcgcg caccgtggat cggaatgacg cgaggtttct
     1021 ggaacgacga cgagcgctac atgcagacgt actggtcgcg gttcccggac gtctggcttc
     1081 acggcgactg ggccgccatc gacgaggacg gcctgtggta catcctcggc cgatccgacg
     1141 acacgatcaa aatcgccggc aagcgcctcg gccccgcgga agtcgaatcg gtgctggtgg
     1201 acgacccgtc ggtcctcgag gcggcggccg tcggcgtgcc ggacgagctc aaggggcagg
     1261 cgctcgtgtg cttctgcgtg ctccggcccg gccacgaagc cggccccgcg cttcggagcc
     1321 gtctgagcgc gctcgtggtc gatcggctcg gcaagccgct tcagccacgc gcgatcgagt
     1381 tcgtcggcga cctgccgaag acgcgcaacg ccaaggtcat gcgccgcgtg atccgggccg
     1441 cgtacatcgg cgaggcgccc ggcgatctct cgtccctcga gaaccctcag gcgatcgacg
     1501 agatccgggc gacaagggcg atcagaaact gaagtcgccg ctgtccgacg gcagcagacg
     1561 agtcacgctt acgacggcgg acaccggcac cgcgccgtag acgtgcggaa acagctcgcg
     1621 ccctccttcc aggttttcgt acttgacggg cgcctgaagg cgagcgggat cgatgtggag
     1681 cagcacgaga tccgaacggc cgcggaaccg ctggttcgcg acccacgcga cctgatgcgg
     1741 ctcggagcag tggatgaagc cctcgctggc cagcgaatcc gcgccgtagg ttccctgtgc
     1801 ttgcgcgtcg gtccacgcct gttccgacgt gatgtggaag atccgtacgt cactcgttcc
     1861 gcctacgacg tcgggcgttg aacgccgagc cgccgcgcgc tcggcggcgt cgtccttgcg
     1921 gcctcctcga tcaggcagct cccaggcaaa gtcgtagaag actcgaaagc ggttgccgtc
     1981 caggtccctc gcggtgaact cgtgcagatg ccagggctgt gactcgggct tcgacacaat
     2041 ctgcgcaccg cgaccgctcc acgactcgta caacgcgtct acgtctgcct tgctgtccag
     2101 gttgatccag atcaccattg gcgtcccggt gctgtcgtct ccgcgaaagg cgttgtcggt
     2161 caggaagatt ctgcagtctc ccctggatac ctggccgagc ccctctgcgc cccaatccca
     2221 gtggaagccc aggcagtttt cgtagtaaac ggcggctcgg gccacatcgc tcacgcgtac
     2281 ctcaggtacc gcgcccggga acttttcact cattgtggct acccgtcgcc cttcatcagc
     2341 tccgcccgcc acctggccgc cgcggtgaac accgcttcga cgaccccttc ttgctgcaat
     2401 ccgccgcgca gttgctcgag ccgatcgatc agggcatcga gcgctacgcc gatgacgtcg
     2461 gcgttggagg cgcagacgcc gcgccagatg tcgggagagc tcgaggccag ccgcgtcgtg
     2521 tccacgagcc cgcggccggc cagcttgagg ccgctcgccg tcacggcgtt tcccacgatc
     2581 tccatgagga cgctggcggt cagctggggc aggtggctca agaacgccat cagccgatcg
     2641 tgttctcccg cctccatcgt cgttggccgc gcgccgagtc cgcgcgcgaa gtcacacagg
     2701 cgatcgaccg cctgcgtcgg cgccgggctg cctggcgtga acacccacgg acgtccccgg
     2761 aacatgtcgg ctcgcgcgaa gctgaagccg ccccgttcgg ccccgccgac cggatggccg
     2821 ccgacgaagg tcgggtgtgg cacgctcagc gcggccgctg cttcgaggat gtcgcgcttg
     2881 gtgcccccga cgtccgtgat caccgcgtca gggccgaggc agcgggcggc ctcgggcagc
     2941 aggcggacgt tctgatcgac cggcgccgca agcaccagca agtcagatcc tttcagatcg
     3001 ctcaggtcct gcacggtacg atcgatcgcc ccgctgccca tcgcgtgcgc aagcacgggc
     3061 ccacggtcca tcgccgcgac gcgcacggac ggccagcgtt cgcggaccgc cagcgcgatc
     3121 gaaccgccaa tcaggccgag gccgacaatc gcgatgttct gaaagggagg atcgtctgac
     3181 atggtcaact tccaactccc aatccccaac tcccaaatcg acgatgcctc ttgggagttg
     3241 ggagttgggc tttgggagtt tggcgttagc tacctcgccc agcctcggcg ggccaccggc
     3301 tcgaggcaga tgctccggcc gatcgccggc gcgatgatgc gcagctccgc catcagcctg
     3361 tcgaactgcg cgggaaacat cgactgcgcc ccgtcgctga gcgcgcgatc gggatcgttg
     3421 tgcacttcga tgatcaggcc gtcagcgccc gccgccaccg ccgcgcgcgc gagctgggcc
     3481 accatgtccc ggcggccggc gccgtggctc ggatccgcga cgatcggaag gtgcgacagc
     3541 ttcttcacca cgggaatcgc cgagatgtcg aacgtgttgc gcgtggcggt ctcgaacgtt
     3601 cggataccgc gctcgcagag gatcacgtcg ttgttgccgc cggccaggat gtactccgcc
     3661 gacaacagcc attcctcgat cgtcgccgag atgcctcgct tgagcagcac cggcttgcgc
     3721 acgtggccca gctcccggag cagcgtgtag ttctgcatgt tcctggcgcc aacctgaaag
     3781 atgtccgagt agcggccgac cagttcgatc tggctgatgt ccatcacctc ggagatgagc
     3841 ttcaggttgt gggcgtcggc ggcgcgccgg agcatctgca ggccctcttc gccaaggccc
     3901 tggaagctgt agggtgagct tctcggcttg aaggcgccgc cgcgaagaat cttggcgccc
     3961 gcgcgcttga ccgcggccgc gctggccatg acctgctcct cggtctctgc cgagcacggt
     4021 cctgccatca cgatcacttc gtcgcccccg acgcgtacgt cctccagcgc gaccacggtg
     4081 tcttccggct tgaagctgcg gctggcgcgc ttgtagggct ccgagatgcg gagcacttcg
     4141 tgcactccgt cgaggatgcc gatgagctcc gggtccacct tgtgcgcgcc cacgacgccc
     4201 agcacggtgc gcgtggcgcc ggaggaccgg tgcacgtcca tgccgagctc gatcaaccgt
     4261 gcgatgaccg cgtcgatctg cccctctgtg gcccgctcct tcatgacgac gaccatgact
     4321 actctcccat ccctgaacgt gaaccgtccc gccgatcgct tgcgacgcgc tccgcccggc
     4381 gcgcttcgtc gatcacccgc tcgaagatcc gcaccaccgc ctcggccgtc agcggcccgg
     4441 cgagatcggt ggccgagcgc ctcaccttgt tgagcacttc ggcctcgcgc tccggctggt
     4501 aaatcgggag cgccgcctcg tgcttcagcc ggccgacttc gaccgcgcac gccacccgta
     4561 cgttcagcag gcgcacgagc tgttcgtcga tcacgtcgat ccggttccgc aactcctcga
     4621 tggtcaggac ttcagccaac gcacgtactc ctccacccgc gtgttgagat cgtttgtcgc
     4681 cccggcctcg gccatgacgc tgacgagcgc gctcccgacg acggcggcgt ccgcccactg
     4741 gcccacttcc cgaacgtgct cggggctgga gatgccaaag ccgagcgcca ccggcagccg
     4801 gctgacacgc ctgatgcgct ccaccatctc acgggcgcct gcggcgatgc ggtcgcgcgc
     4861 gcccgtcacc ccaaggcgcg aaatggcgta gagaaaaccg ctgccgaggg ccccggcttt
     4921 tctcaggcgg tcgtcggtgg tggtcggact gagcaggaga attgtatcaa tcccccgcct
     4981 ccgcagcatg ttccggaagt cgtccgcctc ctcgatgggc aggtcgagca ccagcacgcc
     5041 gtccacgccc gcctcgcggg cgcgatcggc aaatcgctcc gtgccaagcc ggaggatcgg
     5101 gttggcgtag ctgaagatga ccatcggcgc cctgaggtcg gcccgcgcct cggctaccag
     5161 atcgaggacg cgggtgagcg tcgtgccgga ggcgagcgcc cgctcggtcg ccctctggat
     5221 gaccggcccg tccgccagcg gatccgaaaa cggcacgccg atctcgacta cgtcggcacc
     5281 ggcgcgatcc agcgcgctca gcactccgcg tgtcttcgaa aggtccgggt cgcccgccgt
     5341 gatgtaggtc accaaccccg gcttgccgcc ttcccggatc cgcgcgaacg tctcctccag
     5401 ccggctcatc gaagcgcctc cgccacgctc tgaacgtctt tgtcgccgcg ccccgacagg
     5461 ttcacgagga cgaccgaacc atccggaagg tcgcgggcaa gacggcaggt gtacgccacc
     5521 gcgtgcgccg actcgagcgc cggcagaatg ccctcgagac gcgcgagcgt ctgaaacccc
     5581 tcgagcgcct ccgcgtcggt cgcgtaggcg tactccgttc gtccgaggtc gcgcagccac
     5641 gcgtgctcgg gcccgatggc cgcgtagtcg agtcccgccg aaatcgagtg ggtgagctcg
     5701 atgttgcccg cctcgtcctg gaggacgaac gtccgcgtgc cttgcagcac gccggtgctg
     5761 ccgccggcga accgggcagc gtggcggccc ggggagatgc tctcgccacc ggcctcaacg
     5821 ccgatcaacc tcacggatct gtcgtcgagg aacgcgtcga agatgccgat ggcattactg
     5881 cctccaccaa cgcacgccac gacgacgtcg ggaagccggc ccgtctgttc gagcatctgc
     5941 ctgcgggcct cgcggccgat caccgactgg aactcccgga ccatcagcgg gtatggatgc
     6001 gggccaagcg ccgacccgag caggtagtag gtgttgccaa cgttgctcac ccagtcgcgc
     6061 atcgcctcgt tgatggcgtc cttcaacgtc ctgctgccgg agtcaactct ccgcacggtc
     6121 gcgccgagca gctccatccg caccacgttg agcgcctgcc ggcgcatgtc ctccgcgccc
     6181 atgtaaacgc agcactccag tccgagaagc gcgcacgccg tcgccgtagc gacgccgtgc
     6241 tggcccgcgc ccgtctcggc cacgacgcgc gtcttccgca tgcgccgggc cagcagcgcc
     6301 tggccgagtg cgttgttgat cttgtgcgcg ccggtgtgcg tcaggtcttc gcgtttgagc
     6361 aggatgcgca ccctgctcgc cccactcgcc ccgacgacgc cgccggcctc gatggccagc
     6421 cgctgcgtct cgtagagcgg cgtcggccgg cccgcgaagt ggcgcagcag ccgcgacagc
     6481 tcgtcgccga agctcgcatc ggcgcgcgcc tcgagatagc ccgccgtcag atcctcgatc
     6541 ggcgcaacga gcgtttcggg aacgaagcgt cctccgtacg cgccgaagta cccgcgggcg
     6601 tccggatcgc gagcgccgaa gacggggccg tccaggttcg cagggttcac ggggttcata
     6661 aggttcgttt ctgcagcgcg gcaaacgcgc gacgggcgct cgacaggaac gacgacactc
     6721 tctccgcgtt cttcacgcct ggcgactcct ccacgcccga cgacacgtct acgccgacgg
     6781 ggctcacggt tcgaatggcc tgctcgacgt tgaggggcgt cagcccgccc gccaggacga
     6841 ccctgtgatg ccgcgccacc tccgccgcgc gtgcccagtc gatgacgacg cccgtcccgc
     6901 cgcggcggac cggatcggcg acatccagca gcagggtcgt gtcctccggc cagacgctcg
     6961 attgctcggc cgcgtcatcg agcgacaccg atttcaagat cggcagatcg atgccgtcgg
     7021 cgtcctgcgg cttctcgtcg ccgtgcagtt gcacggcgtg gatgcctgtc gcctcgacca
     7081 cccgtcgaat gccgtccgcc gactcgttca cgaagacgcc caccatcgtc acgtgcgccg
     7141 gcagcgtagc gatgatgtcc ctcgcccgct cacggctaat cacccgtgga ctcctcggcc
     7201 agaacacgaa cccgagcgcc gtcgcgcctt cctccacggc gtgcaacgcg tcggcggcgc
     7261 gcgtgatccc gcagatcttg ataatggggt cgggagtcat tttccgcgga cactcccgtc
     7321 ggaaaatgac tcccgacccc ctaacagttc cgtaatcgcc tttgccggat ccggcgccgt
     7381 catgaatcgc tcgccaacga ggaacgcgcg gtatccctgg ctcgacagct cccgcagctc
     7441 gccgtgcgtg ctcaggccgc tctcgctgac ggcgatcacg tcagccggca tccggccggc
     7501 aagccgccgc gaggcgttca catccacgct cagcgtgcga aggttccggt tgttcacgcc
     7561 aacgacctgc gctccgctgt cgatcgcccg cgtcagctcc tcttcgtcgt ggacctccac
     7621 gagcgccgcg agcccgagct cggaggcccg gacctgcagg cgaacgaggt cgtcctgctc
     7681 gagcgccgcc acgatcagca ggacggcgtc ggccccgctc gcacgtgcct ccagcagctg
     7741 atagtcgtcg acgatgaagt ccttgcgcag gagaggcaga tcgacggccc gccggacggc
     7801 cgacaggtgc tcgagaccgc cgtcgaagaa cgtcggttcg gtcaggaccg acacggcaac
     7861 ggccccgccc gcctggtagc agcgcgcgat ggcggccggg tcgtaattgg ccgcgagcac
     7921 tccgcgcgac ggcgagcgcc gcttgcactc ggcgatcacg ttcgtccttc cggccctgcc
     7981 cagcgcggac acgaaacggt cccctcgagg cgacgctgcc gcggcccgcc gctcgagcgc
     8041 ggcctggctc tcgcgttccc gcctcacatc ggtgattcgg cgcgtagcgg ccacgatcgc
     8101 caggagcagg tcgggggcgc gcgtcatgcc actgcctccg cctgcgaggc gtgaaccatg
     8161 cggtcgagcg tcgcgcgtgc caccccggaa tcgatcgcgg cctcggcagc cgcgatgccc
     8221 tcgcgaaccg aacgggcgcg gcccgcaacc agcagcgccg cgccggcgtt gagcacgacg
     8281 atatcccgtg cgggaccgcg ctgcccgtcg agaacgcgcc cgacgatggc tgcattggta
     8341 gacgcgtcac cgccggccag gtcggccggc gacgctttcg cgatcccgaa ctcggtggga
     8401 tggacgaaga acgtatgcac ggcgccgtcg cggcattcgg agaccttcgt gtgcccggtc
     8461 gtcgagatct cgtcgatccc gcccgctccg tgcaccaccc acgcgcgctc ggacccgagc
     8521 tgcatcaacg cccgcgccat cacttgtgtc agctccggcc tgggcactcc gacaatctgc
     8581 ctcgaggctc cggccgggtt cgtcagcggc ccgagcaggt tgaatgtggt cctcacaccc
     8641 atgtcccgcc tgacgggcgc ggcgtgcttc atggacggat gaaaggtctg ggcgaagaag
     8701 aacgcgatgc tcgcatctcg aagcgcccgc tcgacgacgc cgggcggcgc ggcgatgttg
     8761 acgccgagcc gctcgaacac gtccgcgctg ccacattggc tcgacaccga tcggttcccg
     8821 tgcttggcca ccctcacccc gcatgaggcg acgacaactg cggccgctga cgagacattg
     8881 aaagtaccgg atcgatcccc gccggtgccg cacgtgtcga acacctcccc cgccggcgcc
     8941 gacagcttca cggcatgctc gcgcatcgtt cgggctaggc ccacgatctc gtccggccgc
     9001 tcgcccttca tgacgagcgc cgcgagcagg cccgcgagcg cggccggcgc cgcccggccc
     9061 tccatcactt cacgcatggc ggccgcggcc tcctccgtcg tgaggtcctc gtggcgaacg
     9121 agcttctcga tgaggcggga aaacatcaca gatcgaggaa gttccgaaga atcttcctgc
     9181 cctcctccgt cagcaccgat tcgggatgga actgcacgcc atgcaccggg taggtccgat
     9241 gacgcacggc catgatcgtc ccgtcttcct tggttcgcgc cgccacctcg agatccgtcg
     9301 gcacgtcgtc ggatccgatg acgagcgagt ggtaccgtcc cgcctggaac ggctcgttga
     9361 ttccggcgaa cacccccttg ctgtcgtgga ccacggttga cgtcttcccg tgcatcggca
     9421 cctgcgcgcg gcagaccgtg ccaccgtaca cgagcccgat tgcctggtgg cccaggcaga
     9481 ctccgaggat cggcgttcga ggaccgaagc gccggatcac gtccgtcgtg atgcccgcgt
     9541 gctcggggcg ccccgggccc ggcgaaatca gcagctgctc gggcgccatc gactcgattt
     9601 ctcccagcgt cacctggttg ttgcggcgca cgcgaacgtc ggcgccgagc tcgccgaggt
     9661 actgcacgag gttgtaggtg aacgagtcgt agttgtcgat gacgagaatc atgacaggcc
     9721 cgcctgcgcc aactcgagcg cccggatgac cgcgcgcgcc ttgtcgcgcg tctcctcgta
     9781 ttcggccgcg gggttcgagt cggcaacgat tcccgcgccg gcctgcacga acgcccgccc
     9841 cttttcgatc acaactgtac ggatcgtgat gcagaagtcg agattgcctg cgaagtcgag
     9901 atagccgacc gcgcctgcgt agacgcctcg ccgccggttt tccagctcgg cgatgatctc
     9961 catggcgcgc accttgggcg cgccagacac ggtgcccgcc ggaaagcagg ccacgagcgc
    10021 atcgagccgg tcgtgttcct cgcggagccg gccctccacg atcgacacca ggtgcatgac
    10081 gtgcgagtag cgctcgaggg tcatgtaggt cgggacgcgc acgctgccgt actccgcgac
    10141 gcgccccacg tcgttgcgcc ccaggtccac cagcatcacg tgctcggcgc gctccttctc
    10201 gtttcgcttc agctcctcgg caaggcgcag gtcttcttct tcgttgcggc cgcgaggccg
    10261 ggtgccggca atcggatggg tctcgacgcg gcgcccctcg gcgcgcacca gcatttccgg
    10321 cgacgacccc acaatggatc ggccgccgat cttcaggaag tacatgtagg gcgacggatt
    10381 gacgtgccgc agcgcgcgat agacagtgaa gggatcggcc gagatggccg cctcgaaccg
    10441 ctgcgacagt accacctggt agatgtcgcc cgcggcgatg tactccttcg cggtgcggac
    10501 catccgctcg aactcgtcgc gtgtcacgtt cgacgtgacc tcgagcggcg cggcgctggc
    10561 agccggcgct ttcgacagcg tccggtcgag ctcccgctcg acgaagtcga tcttcgtgca
    10621 cgcgaactcg tacagcgact gcaggtcgtc gtcgcccgtg atccgcgcgt tggcgatgat
    10681 gaggatgcgg tgcctcacgt ggtcgaaggc gagcacggtg tcgaagagca tgaagccggc
    10741 ttcctcgtcc gcgtcgttca ccgcctgcag ggtcaccggc tcgaaccacg cggccgcgtc
    10801 gtaggccatg tagcccacgg cgccgcccgt gaagcggggc agcccgggaa cgtacggcga
    10861 atgaaagccc gccatcagct cccgcaccgt cgcgacgaag ggcgaatcgg tctcagtcgt
    10921 ctcgcccatg cgctccacgg tcgtcttccc cccgcgcgat cgcagaatga gaaagggatc
    10981 tttgccgagg aaggagtagc ggcccacctg ttcgccgcct tcgacagact cgagcaggaa
    11041 tgcgtagtcg gaatgctctg cgatcttgag gaacgccgac accggcgtga gcatgtcggc
    11101 gacgatctcc ttgtagaccg gaacgaacgt gccccgcttc gccaggtccc tgaactcttc
    11161 gaacgatgtg atcttcatgc ccttctccgc gcgagccgcc gccgcgccgc cgcctccatg
    11221 actcctggca ccggtcgttg gcctgtccac caggcgaact gcctctctgc ctgtttgacg
    11281 agcatcggga gcccgtccag cgtcgcgcat ccggcggcgc gggcttcctg caggaggacc
    11341 gactcctccg ggccgtaggt gaggtcgtag accagacggc ccgtgaactc cgcacccggc
    11401 atcggcgaca cgttccggaa tcccggcccg ccaagcggcg tgcagttgac cagcagatcc
    11461 catgtgcgtg gtgctggcgg caatgggccc ggcgttgccc cgaacaccgc cgcgatctcc
    11521 tgcgactgct ccgcgcgcct ggcgtggac
//
LOCUS       DBNGSH010000788         3929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4672700, whole genome shotgun sequence.
ACCESSION   DBNGSH010000788 DBNGSH010000000
VERSION     DBNGSH010000788.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4672700"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="AC16CY_18140"
     CDS             complement(<1..686)
                     /locus_tag="AC16CY_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3355777.1"
                     /translation="MQIRPFLPAILVVLAAASGLAQSDAPTYQKPPQAIVDIMDAPPL
                     PGVAVSPLRDVIALVPRRSMPSIAELAKPWIGLAGSRINPSNNGPRGAPSGTGVILRT
                     IATGAESPIRVPADAHIALVGFSPDGKRLAFTDTRDTRVDLYVADVATRATRVADAAL
                     NTIVGGCGWLTDSSALLCSFVASDRGSPPAAPAAPSGPNVQENRGPAGPIRTFQDLLT
                     SAHDEKLFEY"
     gene            complement(714..3506)
                     /locus_tag="AC16CY_18145"
     CDS             complement(714..3506)
                     /locus_tag="AC16CY_18145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355778.1"
                     /translation="MALTIGSRIGVYEVSGALGAGGMGEVYRARDTKLDRDVALKILP
                     DSFATDPERIARFQREAKALAALNHPNVGAIYGMETAAGVTALVLELVEGPTLADRIA
                     GASNDIVGASKDAPLLMRRGGSSDPPVSAIPVDEALAIARQIADALDAAHEQGIVHRD
                     LKPSNIKLRPDGTVKVLDFGLARAMSPDASRSGGALSMSPTITTPAAMTGLGAILGTA
                     AYMSPEQARGKTVDKRADIWAFGVVLYEMLTGHRAFGGDEMSDVFASVLAREPDVALL
                     PKDLPESIRQVLRVCLQKDPKLRARDIGDVKLVMAGTFESVAPAPVVSDTLPRRASPW
                     RAAAWIAASAIIGGAAVGAALWALTPRPATPIARFLLDTPAGPALAFGGAAATDIAIT
                     PDGTRVLYLAAGSGGFDLVVRPVNRLETIVLARQATNPFVSPDGSWVGYATGGEWMKV
                     SITGGPPVSLWKGGGGAPRGASWGPDNTIVFASQGTLGLRRGPAAGGTPEVLTKSDKG
                     DFGHLWPEVLPGGRGVLFTIARNTGLGAEAMEVAVLDLATGQHKTLVRGGSYARYARS
                     GHIVYGVAGTLRAASFDLNRLEVTGDPVPVLEGVMTKQSGAANFSLADDGTLVYVAAR
                     ADTAEQRSLVWVDRTGREETIDAPPRAYLYPRISPDGTKAALDIRDQLADIWIWDFER
                     ETLQRLTNDSGADRAPAWSPDSRRIALSSQAGGAAGNLSWQAADGTGTVERLNDSPNQ
                     QFPAAFTPDGSGLLFWEVGNETRWDMGIVGLKDKRQATHLAKTAFNESNPEVSPDGRW
                     VAYQSDESGQFEVYVRPFPNVDAGGRWLVSNGGGTRPVWAQNGRELFYLVEPGRVMAV
                     PIQPGATFTWGNPQMVFDGPYVAGNAGRTYDVSPDGRRFLMIKDAPRQGKDPAPEPKL
                     VIVQNWVEELKRLVPR"
     gene            3611..>3929
                     /locus_tag="AC16CY_18150"
     CDS             3611..>3929
                     /locus_tag="AC16CY_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355779.1"
                     /translation="MSVRTSVTCGCAVLILLTAGSLSAQTRAANASTGAAVSTWTPEK
                     TPWGDPDLQAIWSGDSAYGIPMQRPPEGGERRQLVPQRQRLARAVVPANLSRRRSAQR
                     PRAG"
BASE COUNT          611 a         1356 c         1312 g          650 t
ORIGIN      
        1 taatactcga acagcttctc gtcgtgcgcg ctcgtcagca gatcctggaa cgtgcgaatc
       61 gggccggcgg gcccgcggtt ctcctgaacg ttcgggcctg acggcgcggc gggcgccgcc
      121 ggcgggctgc cgcgatcgga ggcgacgaag gaacacagca gtgcagagct gtcggtcagc
      181 cagccacatc cgcccacgat cgtgttcagt gcggcgtcgg ctacacgggt cgcgcgcgtg
      241 gcgacgtcgg cgacatacag atcgacgcgc gtgtcgcgcg tgtcggtgaa ggccagccgc
      301 tttccgtcgg gcgagaagcc cacgagcgcg atgtgcgcgt ccgcgggaac gcggattggc
      361 gactcggcgc cggtagcaat cgtccgcaat atcacgccgg tccctgaggg ggcgccccgc
      421 gggccgttgt tcgacgggtt gatgcgcgag ccggccagac cgatccacgg ctttgcgagc
      481 tctgcgatcg acggcatcga ccgtcgaggt acgagagcga tcacatcgcg cagcgggctc
      541 acggccacgc ccggcagcgg cggagcgtcc atgatgtcca cgatggcctg gggcggcttt
      601 tgataggtcg gcgcatccga ctgcgccaga cccgacgcag ccgcgaggac gacgagaatg
      661 gccggcagga acggtcggat ctgcatgact gtttctccgg ttctcccgcg cgattaccgg
      721 gggaccaggc gcttgagttc ctcgacccag ttctggacga tgacgagctt cggctcaggc
      781 gccggatcct tcccctggcg cggcgcgtct ttgatcatca ggaaccgccg gccgtcgggc
      841 gacacgtcgt aggtccgccc cgcattgcct gccacgtacg gcccatcgaa gaccatctgc
      901 gggttgcccc acgtgaaggt cgcgccgggc tggatcggca ccgccatcac ccggcccggc
      961 tcgaccaggt agaacagctc ccggccgttc tgtgcccaca cgggccgcgt gcctccgccg
     1021 ttcgacacga gccatcgccc gccagcgtct acgttcggga acggcctgac gtacacctcg
     1081 aactgccccg attcgtccga ctggtaagcc acccatctcc cgtctggcga gacctccgga
     1141 tttgattcgt tgaaggcggt cttcgccagg tgggtcgcct gacgcttgtc cttcaggccg
     1201 acgatgccca tgtcccatcg cgtttcattg ccgacctccc agaacagcag cccggagccg
     1261 tcaggagtaa aggccgcggg gaactgctga ttcgggctgt cgttcaatcg ttccacagtc
     1321 ccggtgccgt ccgcggcctg ccaggagagg ttgcctgccg cgccgcccgc ttgggacgag
     1381 agcgcaatgc gccggctgtc gggcgaccac gccggtgccc gatcggcgcc agagtcgttg
     1441 gtgagccgct gcagcgtttc gcgctcgaaa tcccatatcc agatatcggc cagctgatcg
     1501 cggatgtcga gggccgcctt cgtgccgtcc ggggagatgc ggggatagag atacgcgcgc
     1561 ggcggagcgt caatcgtctc ctctcgtccc gtcctgtcca cccagacgag cgagcgctgc
     1621 tccgcggtgt cggcgcgtgc cgccacgtag accagtgtgc cgtcgtcggc caggctgaag
     1681 ttggccgctc ccgactgctt cgtcatcact ccttcgagca cgggcaccgg atcgccggtg
     1741 acttccagcc ggttcaggtc gaacgacgcg gcgcgcaggg tgcccgccac gccgtacacg
     1801 atgtgtccgc ttcgggcata gcgggcgtag ctgcctccac gtacgagtgt cttgtgctgg
     1861 cccgttgcga ggtcgaggac ggcgacctcc atggcctccg cgcccaggcc cgtgtttcta
     1921 gcgatcgtaa acagcacacc ccgaccgcca ggcaggactt caggccagag gtgaccgaag
     1981 tctcctttgt cggacttcgt caggacctcg ggggtgcccc ctgccgccgg gccacgccgc
     2041 agtcccaggg tgccttgtga ggcaaagaca atcgtgttgt cgggtcccca actggcgcct
     2101 ctcggtgccc ctccgccgcc cttccagaga gacacggggg ggccgcctgt gatggacacc
     2161 ttcatccatt cgcccccggt cgcatacccg acccagcttc cgtccggcga cacgaacgga
     2221 ttggtggctt gccttgccag cacgatggtt tccaaccgat tcaccggtcg caccaccagg
     2281 tcaaatccac cgcttccggc ggccagatac aacacgcgtg tgccgtcggg tgtgatggcg
     2341 atatcggttg ctgcggcgcc gccgaacgct aacgcgggcc ccgcgggtgt gtcgaggaga
     2401 aagcgcgcga ttggcgtggc tggtcgcggc gtgagcgccc acagtgccgc gccgacagcc
     2461 gctcctccga tgatcgcgct ggcggcgatc cacgctgcgg ctcgccacgg acttgcgcgc
     2521 cgtggcagcg tatcgctgac aacgggcgcg ggggcgaccg attcgaacgt gcccgccatc
     2581 acgagtttca cgtccccgat gtcgcgcgcg cgcagcttgg gatccttctg gaggcagacg
     2641 cgcagcacct gacggatcga ctcgggcaga tccttgggga gcagcgcaac gtcgggctcg
     2701 cgcgcgagca ccgatgcgaa gacgtccgac atctcgtctc caccgaacgc gcggtgtccg
     2761 gtgagcatct cgtagagcac gacgccaaag gcccagatgt cggcccgctt gtccacggtc
     2821 ttcccgcggg cctgctccgg gctcatgtag gcagccgttc ccaggatcgc gcccaggccg
     2881 gtcatcgccg ccggagtcgt gatggttgga gacatcgaga gcgcgccgcc ggaccggctc
     2941 gcgtccgggc tcatcgcgcg cgcgaggccg aagtcgagca ccttcacggt cccgtctggc
     3001 cgcagcttga tgttcgaggg cttcagatcg cggtggacga tcccctgctc gtgcgccgcg
     3061 tcgagcgcat cggcgatctg gcgggcgatc gccagagcct cgtcgactgg aatcgcactc
     3121 acaggcgggt ctgaagaccc gcctctacgc atcagtagcg gcgcgtcttt agacgcgccg
     3181 acgatgtcgt tcgacgcgcc ggcgatgcga tcggcgagcg tcggcccctc gacgagctcg
     3241 agaacgaggg cggtgacccc cgctgccgtc tccatcccgt agatggcgcc gacgtttgga
     3301 tggttgagtg cggcaagcgc cttggcctcc cgctgaaaac gcgcgatccg ctccgggtcg
     3361 gtggcgaagg agtcggggag gatcttcaag gcgacgtcgc gatccagctt cgtgtcgcgg
     3421 gcgcgataca cctcgcccat gccgcccgcg ccgagggcgc cgctgacttc gtagacaccg
     3481 atacgactgc cgatcgtcaa tgccaacgaa agctcgacta ccggcgcgat tatacaggcc
     3541 gacactgcct cggcccgatt ggagcgataa tgcccgcacc cgtcgtggtc gagcgatgga
     3601 aggagacgtc atgagcgtcc gaacatccgt gacgtgcggg tgcgccgttc tcatcctgct
     3661 gacggccggt tcactgtccg cacagacccg tgccgccaac gccagcacgg gcgccgcggt
     3721 cagcacgtgg acgcccgaga agacgccgtg gggcgacccg gatctgcagg cgatctggtc
     3781 cggcgactcg gcgtacggca ttccgatgca gcgcccgccg gagggcggag aacgccgtca
     3841 gctcgttccg caacgacagc gcctggctcg agcggtcgtt ccggcaaacc tctctcgtcg
     3901 tcgatccgcc caacggccgc gtgccggcg
//
LOCUS       DBNGSH010000789         1856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4672705, whole genome shotgun sequence.
ACCESSION   DBNGSH010000789 DBNGSH010000000
VERSION     DBNGSH010000789.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4672705"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="AC16CY_18155"
     CDS             <1..786
                     /locus_tag="AC16CY_18155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HIR3355780.1"
                     /translation="WGAVPTASIGSQFVGFVSARPSDVDNISVFGFEDQTPDMDRVLA
                     ATGFTIDTLTGEIVESDVFFNTRFTWSAAQSGEPGRFDLRSVATHEIGHFFGLGHSAI
                     GETELRADGSHRVVASGSVMFPVSMGTNNIADRTLQPDDIAGMSSIYPDGGFETRTGS
                     ITGRVRLGNAGVLGAHVVAFSIRTGTLVGGFSTTSDGTFTISGLAPGPYVVRVEPLDD
                     YDVDSFLLAPGVNVDFRATFHPRLVVAPSGGANPSFDVTVQPK"
     gene            783..1451
                     /locus_tag="AC16CY_18160"
     CDS             783..1451
                     /locus_tag="AC16CY_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355781.1"
                     /translation="MTSRVPLFVLLSVALVTTEVAAQSVSPPIRRLEVSVGAGFLTGA
                     DLGQQPANERAASGSPYRLFDTETDLSSSLFFGLRLGFALTPRLSIEGAASLGQPELR
                     TSISGDAEGAESLIAVEQMDQYTFDGGIVYRFGGLRGLVPFVSAGAGYQRQRHEGETL
                     IEGGGLFYAGGGVTHWLSVRKQGTLKALGVRGDARLYFLSGGITPDDMRTQAAFAGSL
                     VMVF"
     gene            1548..>1856
                     /gene="acnA"
                     /locus_tag="AC16CY_18165"
     CDS             1548..>1856
                     /gene="acnA"
                     /locus_tag="AC16CY_18165"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506281.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR3355782.1"
                     /translation="MPSLNTFGTRTEIRVGGQPVQIYSLPALEKAGFPGVARLPYSMK
                     ILLENLLRREDGAFVKADDVRAVAQWDASAKVEKEISFMPARVLLQDFTVVPCVVDLA
                     A"
BASE COUNT          281 a          620 c          617 g          338 t
ORIGIN      
        1 tggggggcgg tcccgacggc gtccatcggc tctcagttcg tcggcttcgt gtcggccagg
       61 ccttccgacg tcgacaacat ctcggtgttc ggcttcgagg accagacgcc ggacatggac
      121 cgcgtgctgg cggcgaccgg cttcaccatc gacacgctga ccggagagat cgtcgaatcg
      181 gacgtgttct tcaacacgag gttcacatgg tcggccgcgc agtccggcga gccgggccga
      241 ttcgacctga ggtccgtggc cacgcacgag atcggccact tcttcggtct tgggcactcg
      301 gcgatcggcg agaccgagct ccgggccgac ggctcgcacc gcgtcgtggc gagcggctcc
      361 gtgatgttcc cagtctcgat gggcaccaac aacatcgcgg accgcacgct gcaacccgac
      421 gacatcgccg gcatgtcgag catctacccc gacggcgggt tcgagacgcg aaccgggagc
      481 atcacgggcc gcgtgcggct cggcaacgcc ggcgtgctcg gcgcgcacgt cgtcgcgttc
      541 agcatccgga cggggacgct cgtcgggggc ttctcgacga catcggatgg caccttcacg
      601 atctcggggc tcgcccccgg accctatgtt gtacgcgtcg agcctctcga tgactacgac
      661 gttgacagct tcctgctggc gccgggcgtg aacgtcgact tcagggcgac gtttcatccg
      721 cgcctcgtcg tcgcgccgtc cgggggcgcc aatccgagct tcgacgtgac ggtccagccg
      781 aaatgacgtc tcgtgttccg ttgttcgtac tgctgtcagt tgcactcgtc acgactgagg
      841 ttgccgcgca gtcagtctcc ccgcctatcc ggcgcctcga ggtctcggtc ggcgcaggct
      901 ttctcacggg cgctgacctc ggccagcagc cggccaacga gcgggcggcg agcggctcgc
      961 cctaccgcct gttcgacacg gagaccgatc tctcgtcgtc gctcttcttc ggcctgcgcc
     1021 tcggcttcgc gctcacaccc cggttgtcga tcgagggcgc cgcatcgctc ggccagcccg
     1081 agctccgaac gtcgatctcg ggcgatgccg agggggccga gtcgttgatc gccgtcgaac
     1141 agatggacca gtacacgttc gacggaggaa tcgtctaccg ctttggcggg ttgcggggcc
     1201 tagtgccctt cgtgtcggcg ggagcgggat atcagcgcca gcgtcacgag ggcgagacat
     1261 tgatcgaggg cggcggcctg ttttacgccg gcggcggcgt gacgcactgg ctcagcgtgc
     1321 gcaagcaggg cacgctcaaa gcgctggggg tccgtggcga cgcgcggctc tactttctct
     1381 cgggaggcat cacgcccgac gacatgcgca cacaggcggc gtttgccgga agtcttgtaa
     1441 tggtctttta gtcggtgcga tcccgatcag gctcgcaccg actcgcgtca ggttcgcacc
     1501 gccgcaccga caagcatcgg gatcgcacct gatatcatcg aagagccatg cccagcctga
     1561 acacgttcgg tactcgtacg gagatccgcg tcggcggaca gccggtgcag atctacagtc
     1621 ttcccgccct cgagaaggcc ggttttcccg gcgtcgcgcg tctcccgtac tcgatgaaga
     1681 tcctgctgga gaacctgctg cggcgcgagg acggcgcgtt cgtgaaggcc gacgacgtgc
     1741 gggccgtcgc gcagtgggat gcgtcggcga aggtcgagaa ggaaatctcg ttcatgccgg
     1801 cgcgagtcct cctccaggat ttcaccgtcg tgccctgcgt cgtcgatcta gcggcc
//
LOCUS       DBNGSH010000790         1755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1445430, whole genome shotgun sequence.
ACCESSION   DBNGSH010000790 DBNGSH010000000
VERSION     DBNGSH010000790.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1445430"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..592
                     /locus_tag="AC16CY_18170"
     CDS             50..592
                     /locus_tag="AC16CY_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355783.1"
                     /translation="MRRTLFAAFVMAVVLPAGLAHAQAPPAPAAPPAPASRPAPRPSP
                     PQAAPAKPAPAPLPAGEPVNIRYEVVVFDKGGNQPARKVVTILAAAGEMSMVRADRDG
                     RGNPLGVDVTPSFIRNGKIMTRFGISYVPPPTANESKLEISQNTTLWMESGKPTVISE
                     ATDPISDRRIIVEVTATILK"
     gene            complement(642..1331)
                     /locus_tag="AC16CY_18175"
     CDS             complement(642..1331)
                     /locus_tag="AC16CY_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355784.1"
                     /translation="MQKWLVSVAVLALALTMVGEAQRGGGGRGGAAGGRGGGPPRPVA
                     GNGVEVPGWWARMDNPKEGLTGLKFAPAGGGMHATTGPNIIFWDPQQTASGTYVAKAT
                     FTVTKPPSHEVSYGLFIGGSNLSEDNQKYSYFLIRENGQFLVKKRDGAKTSNVAGDWA
                     PNPAIVAIAGGTQKNELSVQVGGGKVIFSVNGKEVASHAATAVDTDGIVGLRVGHGLD
                     VQIDGFAVERK"
     gene            complement(1402..>1755)
                     /locus_tag="AC16CY_18180"
     CDS             complement(1402..>1755)
                     /locus_tag="AC16CY_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355785.1"
                     /translation="WHAHNVYLHVLAETGALGLAAWCLLWWAIVARLARARRRADAAG
                     RIHLSGALLAVIAFLVLSISEVLIGARVIASLRMNLTVGLIAMMAVHLAKVTQDSKLK
                     TQNSKEAADSTPSEM"
BASE COUNT          291 a          622 c          540 g          302 t
ORIGIN      
        1 gacccgtcga gatcgaaccc ctgaccttcc agcgaagagg agagctccaa tgaggcgcac
       61 gctgtttgcc gcgtttgtca tggccgtcgt gcttcccgca ggcctcgcgc acgcccaggc
      121 cccacccgcc cccgcggctc cacccgcccc cgcgtctcgc ccggcgccgc ggccttcgcc
      181 gccgcaggcc gcgccggcca agccggctcc ggcgccgctg ccagcaggag agccggtcaa
      241 catccgttat gaagtagtgg ttttcgacaa gggaggcaat cagcccgcga ggaaggtcgt
      301 gaccatcctg gccgccgccg gcgaaatgtc gatggtccgc gcggatcgcg acggtcgggg
      361 gaatccgctg ggcgtcgacg tcacgccgtc ttttatccgg aacggaaaga tcatgacgag
      421 attcggcatc tcgtacgtgc cacctccaac cgcgaatgaa tcaaagctgg agatcagcca
      481 gaacacgacc ctttggatgg agagtggaaa gcccaccgtg atttcggagg cgaccgaccc
      541 gatctcggat cgccggatca tcgtcgaggt cacggcgacg atcctcaagt agagcgcggg
      601 cttcagcccg cgcggtctcc gcggaggctg aagcctccgc gctacttcct ctcgaccgcg
      661 aacccgtcga tctgcacatc caggccgtgg cccacgcgaa ggccgacgat gccgtcggtg
      721 tcgacggccg ttgcagcgtg gctggcgact tccttcccgt tgaccgagaa gatcactttt
      781 ccgccgccga cctggaccga cagctcgttc ttctgcgtgc cgcccgcgat cgccacgatc
      841 gccgggttgg gcgcccaatc acccgccacg ttgctcgtct tcgcgccgtc gcgtttcttg
      901 acgaggaact ggccgttctc gcggatgagg aagtagctgt acttctggtt gtcctcggac
      961 agattcgatc cgccgatgaa caagccgtac gacacctcgt ggctcggcgg cttggtcacg
     1021 gtgaaggtcg ccttcgccac gtaggtaccg ctcgcggtct gctgcggatc ccagaagatg
     1081 atgttgggcc cggtcgtcgc gtgcatgcct ccgcccgcgg gcgcgaactt gagccccgtc
     1141 agcccttcct tgggattgtc catgcgcgcc caccagcccg gaacctcgac gccgttgccg
     1201 gccaccggcc gcggaggacc acctccacgg ccgcccgcgg cgccgccgcg gccgccgccg
     1261 ccgcgctgag cctcgccgac catcgtcaag gcgagcgcca gaaccgccac tgagacgagc
     1321 cacttctgca tgggtcctcc ggtatgcaag atgagaccga acgtgatgct gcacatccta
     1381 ttgcagtcgg cctttccgga tctacatctc cgacggtgtc gagtcagcgg cctcttttga
     1441 gttttgagtt ttgagttttg agtcttgagt tactttcgcc aggtgcaccg ccatcatcgc
     1501 gatgaggccc accgtcaggt tcatccggag acttgcgatg accctcgcgc cgatgagcac
     1561 ctcgctgatc gacagcacga gaaacgcgat caccgcgagg agcgctcccg acaagtgtat
     1621 ccggccggcc gcgtcagccc gccgccgcgc gcgcgccagg cgggcgacga tcgcccacca
     1681 gagcaggcac caggccgcca gccccagcgc gccggtttca gcgagcacgt gcaggtacac
     1741 gttgtgcgcg tgcca
//
LOCUS       DBNGSH010000791         4871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2295498, whole genome shotgun sequence.
ACCESSION   DBNGSH010000791 DBNGSH010000000
VERSION     DBNGSH010000791.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2295498"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1082)
                     /locus_tag="AC16CY_18185"
     CDS             complement(3..1082)
                     /locus_tag="AC16CY_18185"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3355786.1"
                     /translation="MTGTSGRHGSSAWDRRAFLTATLGAAAAVRAAAQAQAPQQVSPV
                     PTPREWSTPGPIPYPDPDMVALDPRFRQYIVFNTSIKRLHIGTQWAEGPAWNGVGRYL
                     IWSDIPNNVQMRWIEDDGRVATFRNPSGYSNGNTFDYEGRQLSCEHGGRRVARYEPNG
                     TVTTIAERFDGKRLNSPNDVVVHPDGGIWFTDPFYGIRGNFEGFKAEQESKESVYRVD
                     GKTGQIAKVTDEVGQPNGLCFSHDYKRLYVADTGMPRDLKVWEVDGTRLRNGRRLVQF
                     DIPGTGAPSAPDGIRCDADGNIWAGARPGVQVFAPNGDRIGMIRLPEVCANVCFGGPR
                     RNRLFMTASQSLYAVYVETVGAHIA"
     gene            1134..2186
                     /locus_tag="AC16CY_18190"
     CDS             1134..2186
                     /locus_tag="AC16CY_18190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3355787.1"
                     /translation="MLVRLTVAALIGGLVTAHGAAARQAPAAAVERPAVAFDEALGAA
                     KELPRLHSLLASRRGELILERYFNGARATRDANVKSVAKSVISALVGVAIDRGLIPAT
                     ETPIRTYFPELAKDRDDRKQRITIEDLLTMRSGLESTSNRNYGAWVQSRNWVQHALAK
                     PVFTLPGTEMEYSTGNTHLLSAILTKATGTSTWQFAQNALARPLGFTLPRWPQDPQGI
                     YFGGNDMLLTPRQMLAFGELYLNGGRARGGQVVPSRWIETSFVPRGYSRISGQRYGYG
                     WWMRELAGHQAYFAWGFGGQYIVIVPALELVVVTTSASTVAEDRRSHRRTLFELVEDL
                     VIAPVAAAQGAATPRS"
     gene            2183..2914
                     /locus_tag="AC16CY_18195"
     CDS             2183..2914
                     /locus_tag="AC16CY_18195"
                     /inference="COORDINATES: protein motif:HMM:NF016367.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HIR3355788.1"
                     /translation="MKLLVSVANAEEAVAARDGGADLVDAKDPSSGALGALSLPTLRE
                     IHAVVAGARPLTAALGDAEDERYIERKAFEYAAAGCTFVKVGFAAASSTARTGSLLAT
                     AVRGARHGSGDRCGVVAVAYVDEYDVMSGAPADLIDLASREGARGVLLDTANKSGPGL
                     TRLVRPRALRAWVSRAHDRGLLTAVAGRLTLEDLPSVQDSGADIAGVRGAACEGGRTG
                     RVTATNVRLLKERLLSSEAVVLGLG"
     gene            3031..3378
                     /locus_tag="AC16CY_18200"
     CDS             3031..3378
                     /locus_tag="AC16CY_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002798095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3355789.1"
                     /translation="MPEKPVSWLGSSLEDLRAFPEDARRAAGYQLGRVQQGLLPTDWK
                     PMTMVGPGVIEIRVHTRLEHRVFYIAKFEEAVYVLHAFEKRTRQTPQSEIVLAQRRLA
                     DLIRRRVQKPERA"
     gene            3375..3674
                     /locus_tag="AC16CY_18205"
     CDS             3375..3674
                     /locus_tag="AC16CY_18205"
                     /inference="COORDINATES: protein motif:HMM:NF025120.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3355790.1"
                     /translation="MTTKIRRSSANVFRDLGFSAEEATNLKIRSDLMIRLSKVIETRG
                     LTQAQAANVFGVTQPRISDLVRGKIDRFSIDTLIAMLGHAGVRVQIVVGRSSKVA"
     gene            complement(3893..4162)
                     /locus_tag="AC16CY_18210"
     CDS             complement(3893..4162)
                     /locus_tag="AC16CY_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355791.1"
                     /translation="MDNPLISIAVLAGIATGVYALDRLGLWLEARGWIYYRTKRGSVS
                     LGMAVLETQALLDPAKRHMLEATQTEPDEGDSGDRPPPDRVVGKP"
     gene            complement(4489..>4871)
                     /locus_tag="AC16CY_18215"
     CDS             complement(4489..>4871)
                     /locus_tag="AC16CY_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355792.1"
                     /translation="LAMDQYAQLIASRLEGGVLITEPGEIAAALDGGSIPVLAPYRWL
                     RKVDPLPHCWKVTSDSIAAWVAGQIDAGRLVLVKPAGAAGDELVDMYFRQVLPDDIAS
                     VVVAADRIEAWGKEHRARAEAGHR"
BASE COUNT          815 a         1613 c         1586 g          857 t
ORIGIN      
        1 gctcaggcga tgtgcgcacc aaccgtttcg acgtacaccg cgtacagcga ctggctcgcc
       61 gtcatgaaca gcctgtttcg tctcggcccg ccgaaacaga cgttcgcgca cacttcggga
      121 agacgaatca tcccgatgcg atcgccgttc ggcgcgaaca cctgaacgcc gggacgcgcg
      181 ccggcccaga tgttgccgtc tgcgtcgcat cgaatcccgt cgggcgccga cggcgcgccc
      241 gtgcccggta tgtcgaactg aacgagcctg cggccgttcc tgagccgcgt gccgtccacc
      301 tcccacacct tgagatcacg cggcatgccg gtatcggcta cgtacagccg cttgtaatcg
      361 tgtgagaagc acaggccgtt tggctggccg acttcatcgg tcaccttggc gatctgtcct
      421 gtcttgccgt ccacgcgata cacggactcc ttcgattcct gctcggcctt gaatccctcg
      481 aagttgccgc gaatcccgta gaacggatcg gtaaaccaga tgcctccgtc gggatgcacg
      541 acgacgtcgt tcggtgagtt gaggcgtttg ccgtcgaagc gctcggcgat ggtcgtgacg
      601 gtgccgttcg gctcgtaccg ggccacgcgg cgcccgccgt gctcgcacga aagctgccgc
      661 ccctcatagt cgaaggtgtt gccgttgctg tagcccgacg ggttccggaa cgtcgccacg
      721 cggccgtcgt cttcgatcca tcgcatctgg acgttgttcg ggatgtcgct ccagatgaga
      781 taccgtccca cgccgttcca tgcgggcccc tcggcccact gcgtgccgat gtgcagccgc
      841 ttgatcgacg tgttgaacac gatgtactgg cggaaccggg gatcgagcgc caccatgtcg
      901 ggatcgggat acggaatggg gccgggcgtg gaccactccc gcggcgtcgg caccggcgac
      961 acctgttgcg gcgcttgcgc ctgcgcggcc gcgcgcacgg ctgccgcggc gccgagcgtg
     1021 gcggtcagaa acgcgcgccg atcccacgca ctactcccat gcctgccaga cgtgccagtc
     1081 attcggccct ccacccggcg tgaaatctac accagaatcg ggcacactac ggcgtgctcg
     1141 tacggctgac cgtcgccgcc ctgattggcg gcctcgtgac cgctcacggc gcggcggcaa
     1201 gacaggctcc cgcagcggcc gtcgagcgcc ccgccgtcgc ctttgacgag gcgctcggcg
     1261 cggcgaagga gctgcccagg ctccacagcc tgctcgcgag ccggcgcggc gagctgatcc
     1321 tggagcggta cttcaacggg gctcgcgcca cgcgagacgc caacgtgaag tccgtggcca
     1381 agagcgtgat ctcggcgctc gtgggcgtgg ccatcgatcg cggcctcatt cccgcgaccg
     1441 agacgccaat tcggacctac ttcccggaac tggcaaagga tcgcgacgat cgcaagcagc
     1501 gcatcacgat cgaagacctt ctgacgatgc gctcggggct ggagtcgacg agcaaccgga
     1561 actacggcgc gtgggtgcag agccgcaact gggtgcagca tgcgctcgcg aagccggtct
     1621 tcaccctgcc gggcaccgag atggaataca gcaccggcaa tacacacctg ttgtcggcga
     1681 tcctgaccaa ggccacgggc acgagcacgt ggcagttcgc acagaacgcg ctggcgcgcc
     1741 cactggggtt cacgctgccg cgctggccgc aggatccgca gggcatctac ttcggcggca
     1801 acgacatgct gctgacgccg cgccagatgc tggccttcgg ggagctgtat ctgaacggcg
     1861 gacgcgcgcg cggcgggcag gtcgtgcctt cgcgctggat cgagacgtcg ttcgttccac
     1921 gcggttactc gagaatcagc ggccagcgct acggctacgg gtggtggatg agggagctcg
     1981 ccggacacca ggcgtatttc gcctggggat tcggcggcca gtacatcgtg atcgttcccg
     2041 cgctcgagct cgtggtcgtg acgacgtcgg cgtcgacggt ggccgaggac aggcgcagcc
     2101 accgcaggac gctgttcgag ctcgtggagg atctcgtgat cgcgccggtt gcggcagcac
     2161 agggagcagc cacccctcgt tcatgaaact tctcgtcagc gtcgccaacg cagaagaggc
     2221 agtcgcggca cgggacggcg gcgcggatct cgtcgacgcc aaggatccgt cgagcggcgc
     2281 tctgggtgcg ctgtcccttc cgacgctccg cgagattcac gccgtggtgg ccggcgcgcg
     2341 gccgctgacg gccgcgcttg gagatgccga ggatgaacgc tacatcgagc ggaaggcctt
     2401 cgagtacgcg gcagccggat gcacgttcgt gaaggtcggt tttgcagccg cgtcgagcac
     2461 ggctcgaacg ggttctctcc tggccacggc cgttcgtggc gcgcggcatg gaagcgggga
     2521 tcgctgcggg gtcgtggctg tcgcctacgt cgatgaatac gatgtgatga gcggtgctcc
     2581 ggccgatctg atcgacctcg cgtcgcgcga gggagcgcgc ggcgtgctgc tggacacggc
     2641 aaacaagagt ggacctggcc tgactcgact ggtgcgtccc cgcgcgctca gggcgtgggt
     2701 ttcacgagcg cacgacaggg gcctgctcac cgccgtcgcg ggccgattga cgctggagga
     2761 tctgccttcg gtccaggact cgggcgccga cattgccgga gtgcgcggcg ccgcgtgcga
     2821 gggggggcga accggccggg tgaccgcaac gaacgtgcgc ctcctgaagg agagactcct
     2881 ctcatcggaa gcggtggtcc tcggcttggg ctaaacgtca cgccccaaca cttcggcagc
     2941 cgtcacgtca gacagcacgg gcatgacgcc gcccgcctcg agagtgtcag gcaacttgat
     3001 cgatttatat aaatacgtat atagtactcc gtgcccgaga agccggtgtc ctggctcggc
     3061 tcgtcactcg aggacctccg agcgtttccg gaggatgcgc gtcgggccgc gggataccaa
     3121 ctcggccggg tgcagcaggg cctcctgcca accgactgga agccgatgac catggtaggg
     3181 ccaggagtca tcgagattcg tgtgcacacc cgtctggagc atcgcgtctt ctacatcgcc
     3241 aagttcgagg aggccgtgta cgtgctccac gcgttcgaga agcggacccg ccagacacct
     3301 caatcggaga tcgtgctggc ccaaaggcgg ctcgccgacc tgatccgtcg gcgcgtccag
     3361 aagccggagc gcgcatgacc acaaagattc gacgttccag cgccaacgta ttccgagacc
     3421 tcgggttttc agccgaggag gcaacgaatc tcaagatccg gtccgatctg atgattcgcc
     3481 tgagcaaggt gattgaaacc cgcggcttga cccaagcgca ggccgcgaac gtcttcggcg
     3541 tgacgcagcc gagaatcagc gacctcgttc ggggcaagat cgatcgattc agcatcgaca
     3601 cattgattgc catgctcggt cacgccggcg tcagggttca gatcgtcgtt ggtcgttctt
     3661 cgaaagttgc ctgacgtcag cttgagcagc gccgcgtcac gaatgcagcg cggcgtcccc
     3721 tgcaagctgt tggcgacttt gtcgtggcga tgcactcaac gcgttccccc gaccgccgtc
     3781 tgcatcgatg gatgcgccgt ctgtacgcca atttgcgccg tgccgctggg cggtgtcgca
     3841 taacggcaac cgggtcaacc ttcccgcgga gcggcaggac cgcgaaacgg cgctaaggct
     3901 tcccaactac tcgatctggg ggtggccgat cgccagagtc cccttcgtcc ggttcggtct
     3961 gggttgcctc cagcatgtgc cgcttggccg gatcgagcag cgcctgcgtc tccagcacgg
     4021 ccattccaag tgacacgctc ccgcgcttgg tccggtagta gatccacccg cgcgcctcaa
     4081 gccacaggcc gagccggtcg agggcataca ccccggttgc gatccccgca agcaccgcga
     4141 tcgagatcag cgggttgtcc atgcgtatct catgttgtcg ccggccggct ccggccgcaa
     4201 gccgaggttt taggcgtcgg cgtcgtgtgg tccggccgcg aaattcgtca ccgggctgct
     4261 tggaccatag agcctctctg ggacacttca tcgtgggccg tgactgcgaa tgggtagacg
     4321 tgacgcgtgc tgttcgtggc gtgcttagat tgatcttttc ttccgattct cgccaacatc
     4381 gtacaggtgg aaggcgcaaa ttgcagttgc ttccacgaag acctcgctcc cgtactcccg
     4441 ctgagcgccg tcattctcct gggcgcaggc gggtctaaag acccgcctct accgatgacc
     4501 ggcctccgct cgcgctcggt gctccttgcc ccacgcctcg atccgatcgg ctgcgacgac
     4561 gactgacgcg atgtcgtcgg gaagaacctg tcgaaagtac atatcgacga gttcgtcgcc
     4621 ggcggccccg gccggtttca ccagcacgag ccgacccgcg tcgatctggc ccgccaccca
     4681 cgccgcgatg ctgtcgctcg tcaccttcca gcagtgtggg agcggatcca ctttgcgtaa
     4741 ccagcgatag ggcgcgagca ccgggatgct gccgccatcg agcgccgccg caatctcccc
     4801 cggttcggtg atcaggacac caccctcgag cctcgaggcg atgagctgcg cgtactggtc
     4861 catcgccagc a
//
LOCUS       DBNGSH010000792         2752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1445685, whole genome shotgun sequence.
ACCESSION   DBNGSH010000792 DBNGSH010000000
VERSION     DBNGSH010000792.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1445685"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC16CY_18220"
     CDS             <1..186
                     /locus_tag="AC16CY_18220"
                     /inference="COORDINATES: protein motif:HMM:NF022892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 family protein"
                     /protein_id="HIR3355793.1"
                     /translation="GEMSGGFALVAWPSQYDSTGVMTFIVGRDGAVFEKDLGPKTDDA
                     ARNMTTFNPDSSWRRVQ"
     gene            204..1508
                     /locus_tag="AC16CY_18225"
     CDS             204..1508
                     /locus_tag="AC16CY_18225"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3355794.1"
                     /translation="MTPVEQFVAALHAQDVARVSALLEAHAEVRAAINAPISYFDSRP
                     AKCATKNLPLLDLLLSYGADLNLKSAWWAGGFGILEYDMTPEEAAPLIARGAAVDVFA
                     AAHLGMFDRLRELVERDPSLVRARGGDGKTALHSAKTVEMARYLVEKGAEVDVRCVDH
                     ESTPAQYLAVDAPDVVRYLIGLGARIDIFLAVALGDRQLVERCLADDPGALDHRIGEG
                     RFRVRHNGARAATAEEIGDGRGDIYRWVLGHFMTPIEVASQLKRPDVVQQLLGHASPV
                     QRLLAACATGDRSSAERLVASDPGIVSRLTESQRRVIADRAFWNDTAAVALMVDLGFD
                     TRAVGNDNGDALHWASFLGNAEMVRVLLTRDPAIGVRDGSHGGTPLGWCLYGSVFGWN
                     KRSGDYVEVARMLIEAGEVVEPGMLPTGRDDVDRVLRAHLKR"
     gene            1564..2607
                     /locus_tag="AC16CY_18230"
     CDS             1564..2607
                     /locus_tag="AC16CY_18230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3355795.1"
                     /translation="MRYEVAVVTLSAVLAAAVPIAGQVFVAAPQAATQGPPTAAPDGP
                     KPAFEVTSVRPNTGPVNAMSLNSPPGRFTMTGLPLQMLLTLAYSLRPNQVDGLPDWVR
                     TSRFDINAKMPDGADQDQVMLMVQSLLEDRFKLKWHKETREGDVYALVVARKDGRLGP
                     KLTKSDMECKAILEQRQAAMKEAMAKARTLGPKEQQAVLQTLGSKPGEPLICNTSMTP
                     AATPGGPMAMTIKTAGLEFSTTLIALLSSLSGRPVVDRTDLTGGFDFEFTFSPTMMAR
                     GLTTAVPGAPPAGLGGAGGLGAPAGLGAPVPTDDSPTVFQALEEQLGLRLRPERGPLE
                     YFVVDSIQQPEPD"
BASE COUNT          437 a          917 c          956 g          442 t
ORIGIN      
        1 ggcgagatgt ctggaggctt cgcgctcgtc gcgtggccgt cgcagtacga ctcgaccgga
       61 gtcatgacgt tcatcgtcgg tcgggacggt gccgtcttcg agaaggatct gggcccgaag
      121 accgacgacg cggcgcgaaa catgacgacg ttcaaccccg attcgtcctg gcgccgggtt
      181 cagtaacggg tcgcggtcac acgttgacgc cggtcgaaca gttcgtggcc gcgcttcacg
      241 cgcaggacgt cgcgcgcgtg agcgcgctgc tcgaggcgca cgccgaggtc cgtgccgcga
      301 tcaacgcgcc catcagctat ttcgacagcc gaccggcgaa gtgcgcgacg aagaatcttc
      361 cgctgctcga tctcctgctg tcgtacggcg ccgatctcaa tctgaagtcg gcgtggtggg
      421 ccggcggttt cggaatcctc gagtacgaca tgacgcccga agaagcggcg ccgctcatcg
      481 cccggggcgc ggccgtcgac gtcttcgcgg ccgcgcatct cgggatgttc gatcggcttc
      541 gcgagctggt cgagcgcgac ccttcgctcg tacgcgcgcg cggcggcgat gggaagaccg
      601 cgctccattc cgcgaagacg gtggagatgg cgcgctatct cgtcgagaag ggcgcggagg
      661 tcgacgtgcg ttgcgtggac cacgaatcga cgcctgcgca gtacctcgcc gtggatgcgc
      721 ctgacgtcgt tcgatacctc atcggcctcg gcgcccgcat cgacatcttc ctggccgttg
      781 cgctcggcga tcgacagctg gtcgaacgct gcctggcgga cgatcccggc gcgctcgatc
      841 atcgaatcgg cgaaggaagg ttccgcgtcc ggcacaacgg ggcgcgtgcg gcgaccgcgg
      901 aggaaatcgg cgacggacgg ggcgacatct accgctgggt gctcgggcac ttcatgacgc
      961 cgatcgaagt cgcgtcgcaa ctgaagcgcc ctgacgtcgt ccagcaactg ctggggcacg
     1021 cctctccggt tcagcgactg ctggcggcat gtgccaccgg agatcgatcg tcggcggaac
     1081 ggcttgtggc ctccgatcct gggattgtct cgcggctgac cgagagccag cggcgcgtga
     1141 tcgcggaccg cgcgttctgg aacgacacgg cggccgtcgc cctgatggtg gacctggggt
     1201 tcgacacccg ggccgtcggg aacgacaacg gagacgcgct gcattgggcg tcctttctcg
     1261 gcaacgccga gatggtgcgc gtgctgttga cccgcgatcc ggcaattggc gttcgcgatg
     1321 ggtcgcacgg cggcacgccg ctcggctggt gcctttacgg atccgtcttc ggttggaaca
     1381 agaggtcggg cgactacgtc gaggtcgcgc gaatgctcat cgaggccggt gaagtcgtcg
     1441 agcccggcat gttgcccacc ggacgcgacg acgtggaccg ggtgctgcgc gcgcacctga
     1501 agcggtagcg tcaacgagca aactgtcgag tgcccgggca cgtcgaaggt tcaggagccg
     1561 gccgtgcgct acgaagttgc cgtcgtcact ctcagcgccg tcctcgccgc cgccgtgccc
     1621 atcgccgggc aggtgtttgt cgcggcgccc caggcggcga cgcaggggcc gccgacagcg
     1681 gcccccgacg ggcccaagcc cgcgttcgag gtgacatcgg tgcggcccaa caccgggccc
     1741 gtcaacgcga tgtcgctcaa ctcgccgccc gggcgtttca cgatgacggg gctgccgctg
     1801 cagatgctgc tgaccctcgc ctacagcctc cggccgaacc aggtggatgg tttgccggac
     1861 tgggtcagga cctcgcggtt cgacatcaac gccaaaatgc ccgacggggc ggaccaggac
     1921 caggtgatgc tcatggtcca gtccctgctc gaggaccggt tcaaactgaa gtggcacaaa
     1981 gagacgcgcg agggcgacgt ctacgcgctc gtcgtcgcgc gcaaggacgg gcggctgggg
     2041 ccgaagctca cgaagtcgga catggagtgc aaggccatcc tcgagcagcg tcaggcggcg
     2101 atgaaggaag cgatggcgaa agcccggact cttgggccca aggaacagca ggcggtcctg
     2161 cagacgctgg gttccaagcc tggtgagccg ctgatctgca acaccagcat gacgcctgcg
     2221 gcaacgcctg gggggccgat ggccatgacg atcaagacgg ccgggttgga gttctcgacg
     2281 acgctcatcg cgctgctctc cagcttgtcg ggtcggcccg ttgtggaccg caccgacctc
     2341 accggcggct tcgacttcga gttcactttc tcgccgacga tgatggctcg agggctcacg
     2401 acggctgtgc ccggcgcccc gccagccggg ctcggaggtg ccggcggact cggggctcct
     2461 gcgggcctcg gcgccccggt gccgaccgac gacagcccca ccgtgttcca ggcgctcgag
     2521 gagcagctgg gtctcagact gcgccccgag cgcggaccgc tcgagtactt cgtcgtcgac
     2581 agcatccagc agcccgagcc cgactgacgc ccccctgccc cctgccccct gccccctgcg
     2641 taagaaagtc cccccgtctg cgactatctg acgtgcatgc atcgtccggt tggaggcgat
     2701 gagcgcctct actccgaagt ggccgcggaa tacggcggcg cgctggagcg gc
//
LOCUS       DBNGSH010000793         3515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_767276, whole genome shotgun sequence.
ACCESSION   DBNGSH010000793 DBNGSH010000000
VERSION     DBNGSH010000793.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_767276"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..672
                     /locus_tag="AC16CY_18235"
     CDS             172..672
                     /locus_tag="AC16CY_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355796.1"
                     /translation="MRSRAWCPRLGVAIVVVSCLLACAVQTAAAQDQEERSDAVSQTP
                     FWTKRKVVLLGGFGGSIAAGALAWSKEMELRDRKREMQGLPPGAIDEWNRQFSDATNL
                     AGARNVWGVVAISAATVTSLYAMASRPRETRTPGLGPDVHPPGKPIWDLRVNPFLRSV
                     SVEWSF"
     gene            707..1771
                     /locus_tag="AC16CY_18240"
     CDS             707..1771
                     /locus_tag="AC16CY_18240"
                     /inference="COORDINATES: protein motif:HMM:NF040218.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HIR3355797.1"
                     /translation="MLTVRQFTAGATYGLACCIAAVWLAGCTGLSDLTAPTEVSQSAS
                     PAPPPSPPPPTPPPPPTPPPPQPPPPTPPPSCAYTVSPTSASFGSTGGTGSVSVTTGS
                     GCAWNATSSASWIRITSSASGTGNGTVAYTVDANASSTSRSGTLTIAGQTVTVTQAGT
                     APPPPPTPSCTYDVQPTSASFAAAGGAGSVTVTAATGCAWNATSNASWIRITSSPSGT
                     GSATVAYVVDANATTSGRTGTLTVAGQSVTVSQAGAAEPPPPPPPPPPPPSCTYEVQP
                     PTASFGAFGGTGSVTVTVASGCAWTATSQANWITITSGAGGTGNGTVTYTVDLNLLGS
                     RSGSLTVAGQTVTISQQGAL"
     gene            1947..>3515
                     /locus_tag="AC16CY_18245"
     CDS             1947..>3515
                     /locus_tag="AC16CY_18245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3355798.1"
                     /translation="MIPLRVLLIEDSRDDAALVVRELSRAGYAVTSERVDTPEALSLA
                     LKRHRWDIAIADYTMPAFNGAAALSLVREYDPDLAFIFVSGTIGEDAAVAAMQTGAQD
                     YISKSNLTRLVPAVESQLRKAAGRLARAQADAELLKVAYRDALTDLPNRVLLHDRLRQ
                     AILAAQRARKSLALIVVDLDGLKAVSGSLGPEAADVVIRTLTTRLRALLREVDTVARL
                     GADQFALMLPETGSAGAVRAARKVSQDLADPYTIEGQSFPVTGRLGIAVFPEHASHAD
                     ELLQKAERAVPGAGSDGADYAVFVPAGDRDTKGRLGLVTELREGFERNQFSSDYQPIV
                     HLETGRVLSVEALARWNHPSHGRLQPADFIELVEQTWLIEPLTIRLIDRALAEWAPRD
                     GLPWVSVAVNLSLRSLRDPDLPDRIADVLRRHEAAPSALVLEITEHILRQDTPRALSC
                     LSRLHGMGVTLAVDNFGRGESLAGHLRHLSINRLKIDRTLVNGATAGDDANLRSTIDL
                     AHSLNMIVVAEGVES"
BASE COUNT          591 a         1182 c         1173 g          569 t
ORIGIN      
        1 gcggtgctac ggcagccgat gacgcggcca cagctgaccc cgcagcggct gcccgaaagg
       61 gcggcgatgt atgcacaaac gatgtaggaa agcacgaatt caggaatgaa gtgcgggaag
      121 gggttgcgcc cactcccgaa gagggctgag gttcccaaag ggggagacga catgcgatct
      181 cgtgcgtggt gtccgcgtct tggcgtagcg atcgtggttg tctcctgcct tctcgcctgt
      241 gcggtgcaaa ccgccgctgc tcaagatcag gaagaacgtt ctgacgctgt ctctcagacg
      301 ccgttctgga cgaaacgcaa ggtcgtcctc ctgggcgggt tcggcggatc catcgccgcg
      361 ggcgctctcg cgtggtccaa ggagatggag ctgagggacc gcaaacggga aatgcagggc
      421 ctgccgcccg gagcgatcga cgagtggaac cgccaatttt cagacgccac caacctggcc
      481 ggcgcaagga acgtgtgggg cgtcgtcgcc atcagtgctg ccaccgtgac gagcctctac
      541 gcgatggcct ctcgtccccg ggagacccgc acgccaggcc tcggaccgga cgtgcacccg
      601 ccggggaaac cgatctggga tctccgggtc aacccattcc tgcgcagcgt ctccgtcgag
      661 tggtcgttct agcaggcaag gttcaaaact caacgtgaga ggctcaatgc tcaccgttcg
      721 acagttcacg gcgggcgcca cgtacgggct ggcttgctgt attgctgcgg tctggctggc
      781 cggatgcacc gggctgagcg accttacggc gccgaccgag gtgtcgcagt ccgcttcgcc
      841 ggcgccaccg ccatcgccac cgccgccgac accaccgccg ccgccgacac caccgccgcc
      901 acaaccgcca ccgccgactc cgccgccgtc gtgtgcctac accgtcagcc cgacatcggc
      961 atcgttcgga agcacaggcg gcaccggcag cgtgagcgtg acgacgggca gtggatgtgc
     1021 gtggaacgcg acaagcagcg cgagttggat tcggatcacg tcgagtgcga gcggcacggg
     1081 gaacgggacc gtcgcctaca cggtcgatgc gaatgcctca tcgaccagtc gctccggaac
     1141 cctgaccatc gccgggcaga cagtcaccgt gactcaggcg ggtacagcgc ccccgccgcc
     1201 cccgactccc tcctgcacgt atgacgtgca gcccacgtcc gcttccttcg ctgccgccgg
     1261 gggggcgggc agcgtgaccg tgacagcggc caccgggtgt gcgtggaacg cgacgagcaa
     1321 cgcgagttgg atcaggatca cctcgagccc gagcggtacc ggcagcgcca cagttgccta
     1381 cgtcgtcgat gcgaatgcca cgacgagcgg ccgtactgga acgctgacgg tcgccggcca
     1441 gtccgtcact gtctcgcagg cgggtgccgc agagccgccc ccgccgccac caccaccacc
     1501 gccgcctcct tcctgcacct atgaggtgca gccgccgacg gcgtcctttg gcgccttcgg
     1561 cgggacgggc agcgtgactg tgacggtggc aagcgggtgt gcgtggaccg cgacgagcca
     1621 ggcgaactgg atcacgatca cgtccggtgc cggcggcacg ggtaacggca cggtgacgta
     1681 taccgtggac ctcaatctcc tcggcagtcg cagtggaagc ctgacggtgg ccggccagac
     1741 cgtcacgatt tcacagcaag gtgcgctttg agtcgtcgcc cgggcgaacg acgcggacgt
     1801 tcctggaacc agacattccc ccccctgtcg aaaccatcgt gtcgggggtt gcctcgacct
     1861 caccgaatgc aacgggctgc tgctgctcgt ccaggcgctg cgtctcggtc tcggtcggtc
     1921 ggtgtgtctg ggatggcacg tccagaatga ttccacttcg agtgctgttg atcgaagact
     1981 cgcgcgacga tgccgcgctc gtggtgcgcg agctgtcacg ggcagggtac gccgtgacgt
     2041 ccgagcgtgt cgacacgccg gaagcgttga gcctcgccct gaagcggcac cgatgggaca
     2101 tcgcgatcgc cgactacacc atgcccgcgt tcaacggtgc ggcggcgctt tccctggtga
     2161 gggagtacga ccccgatctg gcgttcatct tcgtgtccgg gacgatcgga gaggatgccg
     2221 ccgttgcggc gatgcagacc ggggcccagg actacatcag caagagcaat ctcacgcggc
     2281 tcgtgccggc cgtggagagc cagctccgaa aggccgccgg gcggctcgca cgggcgcagg
     2341 cggatgcgga gctcctgaag gtcgcctatc gcgatgcgct gaccgatctc ccaaacaggg
     2401 tcctgctgca cgatcgacta cggcaagcca tcctggcagc ccagcgcgct cgcaagtcgc
     2461 tggctctcat cgtggtcgat ctcgatggac tgaaggccgt cagcggctcc ctgggccccg
     2521 aggcggccga cgtcgtcatt cgaacgctca ccacccgact gcgcgcgctc ctgcgtgagg
     2581 tcgatacggt tgcgcgcctt ggcgcagacc agtttgccct catgctgccg gagaccggga
     2641 gcgcaggcgc cgtccgagcg gcacgcaagg tgtcgcagga cctcgcggat ccttacacca
     2701 tcgagggtca gtcgttcccc gtcaccggac ggctcggcat cgccgtgttt cccgagcatg
     2761 cctcgcatgc cgacgagctc ctgcagaagg ccgagcgtgc ggtgcccggt gccgggagcg
     2821 acggcgccga ttacgccgtg ttcgtaccgg ccggcgaccg cgataccaag ggacggctcg
     2881 gcctcgtcac cgagctccgc gaggggttcg agcgaaatca gttttcctcc gactaccagc
     2941 cgatcgtgca cctcgagacc ggacgggtct tgtcggtcga ggcgttggcg cggtggaacc
     3001 acccgtccca cgggcgtctg caaccggccg atttcatcga gctcgtcgag cagacatggc
     3061 tgatagagcc gctgacgatc aggctgatcg atcgggcgct ggccgagtgg gccccccggg
     3121 acgggctgcc gtgggtgtcg gtggccgtga acctgtctct caggagcctt cgtgatcccg
     3181 atctgccgga tcggatcgcc gacgttcttc ggcggcacga ggcagcaccg tcggcgctgg
     3241 tgctggagat caccgagcac atcctcaggc aggacacgcc gagggccttg tcgtgcctct
     3301 cgcgtctcca cggcatgggc gtcacccttg cggtggacaa cttcgggagg ggtgaatctc
     3361 tggccggcca cctccgccac ctgtcgatca accggttgaa gatcgatcgg accctggtca
     3421 acggcgcaac ggccggggac gacgccaacc tccggtccac gatcgatctc gcccacagtc
     3481 tcaacatgat tgtcgtcgcc gaaggggtcg agtcg
//
LOCUS       DBNGSH010000794         2206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1445932, whole genome shotgun sequence.
ACCESSION   DBNGSH010000794 DBNGSH010000000
VERSION     DBNGSH010000794.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1445932"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="AC16CY_18250"
     CDS             complement(<1..881)
                     /locus_tag="AC16CY_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HIR3355799.1"
                     /translation="MSTFKAFRIVNDEGRIRGSIVDASLDELTPGGVVIETAYSSVNY
                     KDALAATGAGKILKRFPLIGGIDVSGTVVSSTDGRFGEGDRVLVTGYDLGVSQDGGYA
                     RYVRVPADWVVRVPDGLTLYEAMALGTAGFTAGLSVVRLEQNGLRPGQGRIAVTGATG
                     GVGSVAVAALARLGYDVTAITGKDDAHDYLRELGATEVLSRTTLTMGTRPLETARWAG
                     AVDAVGGDMLSWLTRTTGHWGGIASTGLTGGVEIHTTVMPFILRGVSLIGIDSALCPA
                     GVRTEVWRRLATDMKPQR"
     gene            962..>2206
                     /locus_tag="AC16CY_18255"
     CDS             962..>2206
                     /locus_tag="AC16CY_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3355800.1"
                     /translation="MTPKVRQWIEDGLGDPDAFWARAAGELPWFRTWDRVFEWTYPTF
                     RWFSGAETNLAHNALDYHVARGRGAHTALVYLNERGERRTFTYAELLALVERVSAALR
                     GLGIGKGDRLTIYMPTSPEAIALMLATVRIGAIHSVVFAGFGAAALGGRIDASGSRLL
                     FTCDVTWRKGKELDLKAIVDDSLNTSGQAVEHVIVLERRQAARPRSAREMTWEAFLTR
                     GEGQSSEHTVMEANEAAYILATSGTTAKPKLAVHTHGGYQVHIASMGRWCFGLKPSDV
                     WWATSDIGWIVGHSYIVYAPLMAGCTTVAYEGSLDHPAPDATWSLIAELGVRGIFTSP
                     TGVRLLMRYGTEAAHAHDLSSVERVVCAGEVLNAPAWRWLQEDVFGDRVPVIDHMWQT
                     ETGGPIFGNPYGLGLLPIRPGSA"
BASE COUNT          341 a          759 c          752 g          354 t
ORIGIN      
        1 cgctgcggct tcatgtcggt cgccaggcgc cgccatactt cggttctcac gcccgccgga
       61 cacagcgccg aatcgatccc gatgagcgac acgccgcgga gaatgaatgg catgacggtc
      121 gtgtggatct cgacaccacc ggtcaggccc gtgctcgcga tcccgcccca gtggccggtc
      181 gtacgagtca gccacgacag catgtcgcca cccacggcat ccacggcacc cgcccagcgg
      241 gccgtctcga gcgggcgggt gcccatcgtc agcgttgtcc tggacaggac ttccgtggct
      301 ccgagctcgc gcaggtagtc gtgggcatcg tccttgcccg tgatcgcggt gacgtcgtag
      361 ccaagcctgg cgagcgccgc gacggcgacg ctcccgacac caccggtcgc gccggtcacc
      421 gcgatcctgc cctgcccggg ccgcagcccg ttctgctcga gcctgacgac cgagaggccg
      481 gccgtgaagc cggcggtgcc gagcgccatc gcctcgtaaa gggtgagccc gtccggcacg
      541 cgcacgaccc agtcggcggg aacgcgcaca taccgcgcgt agccgccgtc ctgcgacacg
      601 ccgaggtcgt agccggtgac gagcacgcga tcgccttcgc cgaaccggcc gtcggtgctc
      661 gagacgacgg tgccggagac gtcgatcccg ccaatcagcg ggaagcgctt caggatcttc
      721 ccggcgccgg tagccgcgag ggcgtccttg tagttgacgc tcgagtaggc ggtctcgatc
      781 acgacgccgc cgggagtcag ctcgtcaagc gacgcgtcca cgatcgagcc gcggatcctt
      841 ccttcgtcat tcacgatgcg aaaggctttg aaggtggaca tgggcatgag tgggattcta
      901 cttacgccgg tcggtgcggc gttatgattc ggtttcgttg caaggagaca gcgtgggtgt
      961 ggtgacgcca aaggtccgtc agtggatcga agacgggctc ggggatccgg acgcgttctg
     1021 ggcacgggcg gcgggcgagc tgccgtggtt ccgcacgtgg gatcgcgtgt tcgagtggac
     1081 ttacccgacg ttccgctggt tcagcggcgc agagacgaat cttgcgcaca acgcgctcga
     1141 ctaccacgtc gcacgcgggc gcggcgcgca taccgcgctc gtctacctga acgaacgcgg
     1201 cgagcgacga acgtttacct acgcggagct gctggcgctc gtcgaacgcg tctcggcggc
     1261 tctgcgcggt ctggggatcg gcaagggcga ccgcctcacc atctacatgc cgacgtcgcc
     1321 cgaagcgatc gccctgatgc tcgccacggt tcgcatcggt gcgattcact cggtcgtgtt
     1381 cgcgggcttc ggcgccgcgg ccctgggcgg ccgcatcgac gccagcggct ctcggctcct
     1441 gttcacctgc gacgtgacgt ggcgcaaggg caaggagctc gatctgaaag cgatcgtgga
     1501 cgattccctg aacaccagcg gacaggccgt cgaacacgtg atcgtgctcg agcggcgtca
     1561 ggcggcgcga ccgcggtccg ctcgtgagat gacgtgggag gcgtttctga cgcggggcga
     1621 gggccagtcc agcgagcaca cggtgatgga agccaacgag gcggcctaca tcctggccac
     1681 ctccggcacg accgccaagc cgaagctcgc cgtccatacc cacggcggct accaggtgca
     1741 catcgcgagc atgggccgct ggtgcttcgg cctgaagcca tccgacgtct ggtgggccac
     1801 ctccgacatc ggctggatcg tcggccacag ctacatcgtg tacgcgccgc tgatggccgg
     1861 ttgcaccacc gtcgcctacg agggctcgct cgatcacccg gccccggacg ccacgtggtc
     1921 gctcatcgcg gagctcggcg tgcgcggcat cttcaccagc ccgaccggtg tccgcctcct
     1981 gatgcgctac gggacggagg cggcgcacgc ccacgacctg tccagcgtcg agcgggtcgt
     2041 gtgtgccggc gaggtcctca acgccccggc ctggcgctgg ctccaggagg atgtcttcgg
     2101 ggatcgcgtc ccggtcatcg accacatgtg gcagaccgag accggcggcc cgatcttcgg
     2161 caatccgtac ggcctggggt tgctgcccat caggcccggt tccgcg
//
LOCUS       DBNGSH010000795         4992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3314151, whole genome shotgun sequence.
ACCESSION   DBNGSH010000795 DBNGSH010000000
VERSION     DBNGSH010000795.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3314151"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3237
                     /locus_tag="AC16CY_18260"
     CDS             <1..3237
                     /locus_tag="AC16CY_18260"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355801.1"
                     /translation="YDTQLQRRVAIKFLNGADDESRARLLNEARAASALNHPNICTIH
                     EVAEAGSQPCIVMEYVQGERLETLIPPGGLPPETFFRYSLQIADALMHAHDRGIVHRD
                     LKSANVIVGPEERVKVLDFGLAHRLPEYDTETVTQTVALQAAPGIVAGTIAYIAPEVL
                     KGGKSDTRSDVWSLGMLFYEMASGRLPFKGLTFVELISTIADERPVPPVAELLPSAHR
                     AVIHRCLEKDPERRYKNAGEVLAALRTAQDAPKGLGSRRLAVAGIALLLVAIGAAGAY
                     IRFAPRGGQASAPAPAPVNSRRAVAVLGFKNLSGQPDVAWLSTALSEMLTTELAAGEH
                     LRTIPGENVARMKNDLELADADSFAADTLARINASLGSDLIVMGSYARIGEKVRFDVR
                     MQEAGVGDVIAAVVETGGESELFEIVSRIGTRLRERLGVMELSAAAVAGVQASRPANP
                     AAARLYAEGLAKLRLYDAQAARALLEQAVSADPANPLAHSALALAWSALGYDERARQS
                     AKRAYELSAGLAREDRMWVEGRYHDAMSEYDQAIKTYQALYSFFPDNLEYGLQLATAQ
                     TSAGKGQDALATLDSLRRLPAPVRDDPRIDGAEAAAASSLSDLKRQQAAAARAVAKGR
                     QQGARLLVASARLTEGNAWQELGDAPKAVAAAEEARQIYAAAGDRGGESRALRTAGIV
                     LRSQGDLAGARQMYERGLAVAREIGDRRTTAGLLNNIANMLRQQGSLEAAQKTYAESL
                     AISREIGDRSAVAQTLNNTAILHRVRGDVAGAKKYYLESLDIRRSIGDKAGTNATLNN
                     LANLMGDEGDLAGAMKVYEETLKTAEDLGDKRGVAIAWYNMGETERLQGNLARSRTML
                     DQALTLRRSLEDKSAVARTLTSIGMVLTAQARLTEARRSYEEALALQESVGDKLGTAR
                     ARHFLGSLTLHEGRPEDAESSIREAARQLHELKAFDEEGATLAALARALLAERKNQEA
                     ERTIQEAARLVRGSRNREWLLEVELGLARVEAATGKRAAASTRLRKLQSDARGFTGLE
                     LDAGLALAQVEIASGQAAEGRARLDAIEQQARAKGFLLTANQAAAARR"
     gene            3290..>4992
                     /locus_tag="AC16CY_18265"
     CDS             3290..>4992
                     /locus_tag="AC16CY_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355802.1"
                     /translation="MLAVVIAASLATRVSGQNTPVPATFTTFDIPFDKLPGGRMNAQG
                     NLDPTSSARDAHAGAFVAAKRLGLFRNFEWVHWVPTAPSTRDPATGHWRGGDLDGFPT
                     GWGSTGLGIAGDCLYWGRSNSTNTTGPRVTHDVKIFKIQPDPEKNPPVEVGSMPQLTV
                     DNGETAVRDRELRPYNYTSTDGVERMLMVRSAATGTGGDVITYTLDPQTCLPVDGGAK
                     AEGVLAHEFYLWHDRRNPNRLLVLSQTYGSQDEDLVVTAITDDKTGKVLAKPLFLASF
                     TLEGIGGPVKNERPDSTGLYTGGRFADYRHLTDQWARPGATQTSTVNSLHSGTMSDDG
                     ERFYAAGTTAGTYILNTERIANSTNAQLASGGVCRQRSTNAWVDGKIGGVVDVRKLPE
                     VAGDCVHPVLNSDPGVLAMLRSDRSDADKLARYTRLETRSRFSFTPPLVAMVGVHSAV
                     PIPSRPSLSRGNPKNRPAWVVITEEWPFGPCPERGLRIVNIESEITPMMVGALAQSDS
                     MVQNCLAQAKPPGSTIRPMMHAHNPTVFENIVFVGWLGHGVRAIDISNPFNPREVGHA
                     RP"
BASE COUNT          903 a         1679 c         1679 g          731 t
ORIGIN      
        1 tacgacaccc agctccagcg ccgcgtcgcg atcaagtttc tgaacggcgc ggacgatgag
       61 agccgcgccc ggctgttgaa cgaagcgcgc gcggcttccg cgctcaacca tcccaacatc
      121 tgcacgattc acgaggtcgc cgaagccggc agccagccct gcatcgtcat ggagtacgtg
      181 cagggcgaac ggctcgagac gctgattccg cccggcgggc tgccgcccga gaccttcttc
      241 cgctacagcc tgcagatcgc cgacgcgctc atgcacgctc acgatcgcgg gatcgtgcac
      301 cgcgatctca agagcgcgaa cgtcatcgtc ggccccgaag aacgggtcaa ggtgctcgac
      361 ttcgggcttg cccatcgcct cccggaatac gacacggaga ccgtcacgca gaccgtggcg
      421 ctccaggccg caccgggaat cgtcgctggc acgatcgcct acatcgcgcc ggaggtgttg
      481 aagggcggca agtcggacac ccgcagcgac gtctggtcgc tcggcatgct cttctacgag
      541 atggcgtcgg ggcgtctgcc gttcaaggga ctcacgtttg tcgagctgat atcgacgatt
      601 gccgatgaac ggccggtgcc tccagtggca gagctcctgc cctccgcgca ccgcgcggtc
      661 attcaccgct gtctcgagaa ggaccccgag cgccggtaca agaacgcggg cgaggttctc
      721 gccgctcttc gtaccgcgca ggacgccccg aaggggctcg gatcccggcg gcttgccgtt
      781 gccggcatcg cgctgctgct cgtcgcgatc ggcgcggccg gcgcgtacat caggttcgct
      841 ccccgaggag gacaggcgtc cgcaccggct cccgcgccgg tgaacagccg gcgggccgtt
      901 gccgtcctcg ggttcaagaa cctgtcaggt cagcctgacg tggcgtggct gtccacggcg
      961 ctctccgaga tgctgacgac ggagttggcg gccggcgagc atctgcggac gatccctggc
     1021 gagaacgtcg cgcgcatgaa gaacgacctc gagctggccg acgccgacag cttcgccgcc
     1081 gacacgctcg cccgcatcaa cgccagcctt ggcagcgatc tcatcgtgat gggctcctac
     1141 gccaggatcg gcgagaaggt ccgcttcgac gtgcggatgc aggaggccgg cgtgggcgac
     1201 gtcatcgcgg ccgtcgtcga gacaggcggc gagagcgagc tcttcgagat tgtgtcgaga
     1261 atcggtactc ggctgcgcga gcggctcggc gtgatggagc tgtcggccgc cgctgttgcg
     1321 ggtgtgcagg cctcacgtcc ggcgaacccg gcggcggcgc ggctgtacgc cgaagggctg
     1381 gcgaagctgc gcctctacga cgcccaggcc gcacgcgcgc tgctcgaaca agccgtcagc
     1441 gccgatccgg ccaatcctct tgcccactcc gcgcttgccc tggcctggtc cgcgctcggc
     1501 tacgacgagc gggccaggca gtcggcaaaa cgcgcgtatg agttgtcggc cggtctggcc
     1561 cgtgaggatc ggatgtgggt cgaaggccgg tatcacgacg cgatgagcga gtacgaccag
     1621 gccatcaaga cgtaccaggc gctctacagc ttcttccccg acaatctcga atacgggtta
     1681 cagctcgcaa cggcgcaaac ctccgccggg aagggacagg acgccctcgc gacgctcgat
     1741 tcgttgcggc ggctcccggc tccggtgcgt gacgatccgc gcatagacgg cgccgaagca
     1801 gcagcggcca gctcgctatc ggacctcaag aggcagcagg ccgcggcggc gcgtgctgtg
     1861 gcaaagggac gacagcaggg ggcgcgactt ctcgtggcga gcgctcgtct cacggaaggc
     1921 aacgcctggc aagagctcgg agacgcgccg aaggcagtcg ccgcagccga agaagccagg
     1981 cagatctacg ccgctgccgg cgatcgtggc ggcgagagcc gcgcgcttcg cacggcaggt
     2041 atcgtccttc gctcgcaggg agacctggca ggcgccagac agatgtacga acgcggactc
     2101 gccgtggcgc gcgagattgg cgaccgccga acgacggccg gcctgttgaa caacatcgca
     2161 aacatgctca ggcagcaggg atcgctcgag gcggcgcaga agacctacgc cgagtcactc
     2221 gcgatctctc gtgagatcgg tgacaggagc gcagtggccc agaccctgaa caacaccgca
     2281 atactgcatc gcgtacgcgg cgatgtcgcc ggagccaaga agtactatct cgagtcgctc
     2341 gacatccgac gctccatcgg tgacaaggcc ggcacgaatg ccacgctgaa caatttggcc
     2401 aacctcatgg gggacgaagg cgatctggcc ggggcgatga aagtgtacga ggagacactc
     2461 aagacggcgg aggacctcgg cgacaaacgt ggagtcgcca tcgcctggta caacatgggc
     2521 gagacggagc ggctgcaggg aaacctggct cgatcgcgca ccatgctcga ccaggccctg
     2581 acgcttcgac ggtcgctcga ggacaagagc gccgttgccc gaaccctgac gagcatcggc
     2641 atggtgctga cggcgcaggc acgactgaca gaggcgagac gatcgtatga ggaggcgctc
     2701 gcattgcagg agagcgtcgg cgacaagctc ggcaccgcgc gcgcgcgcca tttcctcggc
     2761 tcgttgacgc ttcacgaagg acggcctgag gacgcggaat cgtcgatccg tgaggccgca
     2821 cgacagctac acgaactgaa agcgttcgac gaggaaggcg ccacgttggc cgcactcgcc
     2881 cgagcgctcc tggctgaacg caagaatcag gaggccgaga ggacgataca agaggccgcg
     2941 cgcctcgtca ggggcagtcg caatcgcgag tggctgctcg aggtcgaact cggactggcg
     3001 cgcgttgagg ctgccacggg gaagcgcgcg gcggcatcaa cccgtctgcg gaagctgcag
     3061 agcgacgcca ggggatttac cggcctggag ctcgacgcag gtctcgcgct tgcccaggtg
     3121 gaaatcgcgt ccggccaggc agcggaaggc cgcgcgcgtc tcgatgcgat cgagcaacag
     3181 gcccgggcga aaggctttct gctgacggcc aaccaggccg ccgcggcgcg gcgctgagtt
     3241 tgcggcgtgc tagtctgtgg cgatcatgac gcgacctctc tctttcgcct tgctcgcagt
     3301 cgttatcgcc gcctcgctcg cgacgcgcgt gagcgggcag aacacgccgg tgccggctac
     3361 gttcaccacc ttcgacatcc ccttcgacaa gctgccgggc ggccgcatga acgcgcaggg
     3421 caacctggat ccgacctcgt cggcgaggga cgcgcatgcg ggggcgttcg tggccgcgaa
     3481 aagactggga ctgttccgca atttcgaatg ggtgcactgg gtgcccacgg cgccttccac
     3541 gcgagacccc gccacgggcc attggcgcgg cggcgacctc gatggctttc cgacagggtg
     3601 gggatcgact ggtctcggca tcgccggcga ctgcctctac tggggacgca gcaattccac
     3661 caataccacc ggcccgcgcg tgacgcacga cgtgaagatc ttcaagattc aacccgaccc
     3721 ggagaagaac ccgccggtcg aagtcggcag catgcctcag ctcaccgtgg acaacggcga
     3781 gaccgccgtg cgcgaccgcg agctgcgacc ctacaactac acgtccacgg acggcgtcga
     3841 gcggatgctg atggtccgca gcgccgccac cgggacgggc ggcgacgtga tcacgtacac
     3901 gctcgatcca cagacgtgcc tcccggtgga cggcggcgcc aaggccgagg gcgtgctcgc
     3961 gcacgagttc tacctctggc acgaccggcg caatccgaac cggctgctcg tcctctcgca
     4021 gacctacgga tcgcaggacg aggatctcgt cgtgaccgcg atcaccgacg acaaaaccgg
     4081 taaggtcctc gcgaaaccgc tgttcctcgc ctctttcacg ctcgagggca tcggcggtcc
     4141 ggtgaagaac gaacgtcccg attcaaccgg cctctatacg ggcggccggt tcgccgacta
     4201 ccgccacctg accgaccagt gggcgcggcc gggcgccacg cagacctcca ccgtgaacag
     4261 cctgcactcg ggcacgatgt ccgacgatgg cgagcggttt tacgcggcgg ggaccaccgc
     4321 gggcacctac atcctgaaca ccgagcggat cgccaacagt accaacgcgc agctggcatc
     4381 gggcggcgtc tgccgccagc gctcgaccaa cgcgtgggtg gacgggaaga tcggcggcgt
     4441 cgtggacgtg aggaaactgc ccgaggtcgc cggcgactgc gtgcacccgg tgctcaactc
     4501 ggatcccggc gtgctcgcga tgctgcgctc ggaccggtcc gatgcggaca agctggcgcg
     4561 ctacacgcgc ctcgagacgc gctcgcgctt cagcttcacc ccgccgctcg tcgcgatggt
     4621 gggcgtgcac agtgccgtgc cgataccgag ccgcccgtcg ctctcgcgcg gcaacccgaa
     4681 gaaccgtccg gcgtgggtcg tgatcaccga ggagtggccg ttcggcccct gcccggaacg
     4741 gggcctcagg atcgtcaaca tcgaatcgga gatcacgccg atgatggtcg gcgctctcgc
     4801 ccagtcggac agcatggtgc agaactgcct cgcccaggcg aagccgcccg gatcgacgat
     4861 caggccgatg atgcacgcgc acaatcccac cgtgttcgag aacatcgtgt tcgtgggctg
     4921 gctgggccac ggcgtccgcg cgatcgacat ctcgaatccg ttcaatcctc gcgaggtcgg
     4981 tcacgcgaga cc
//
LOCUS       DBNGSH010000796         2479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4674059, whole genome shotgun sequence.
ACCESSION   DBNGSH010000796 DBNGSH010000000
VERSION     DBNGSH010000796.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4674059"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="AC16CY_18270"
     CDS             complement(<1..831)
                     /locus_tag="AC16CY_18270"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3355803.1"
                     /translation="MPLIFVSYRRQDTQSATGRLCDKLQQHFDAAQVFHDVESIEPGA
                     DFAATIAAKVSASAVVLVMIGPRWLEAIGSDGRPRLHDPLDYVRLEVVTALQRRLPVI
                     PVLVEGAAMPSASALPDVMRELLRHQAHEITEQRWQYDSDRLVHELEKYVAPNAESAE
                     AAPALGALLLRSMINYPADFVQLLMSPRRQLLERLKGPQFVARAVVFFVLSHLIAAWL
                     FVLEELVASVPAFVLGGVPIGAAHLLLLVIPLHAAARIVRAPSHAPSTTAVLAYMQSV
                     F"
     gene            954..1889
                     /locus_tag="AC16CY_18275"
     CDS             954..1889
                     /locus_tag="AC16CY_18275"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3355804.1"
                     /translation="MSATFGTRLRLQRERQQISLASIAEQTKIRLPLLEALERGDASN
                     WPRGIFGRSYVRAYARAVGLDPDATVREFIECHPEPDESLPPVVGDSRDIGPDKLGRP
                     PIRLQVLIDSAIDAFHARRAEAVERTAPTTEKKLPAAPVDMAPPRHPSPGSGAGPSPV
                     NMWAVADVCTKLGCAREAHELRSALEDASGVLDAAGLILWMPDARRATLTPVFAHGYS
                     DDVLARLPVLHTHDDNATAAAFRTHTTCVVNASHSRHGAVVAPLLTPNGCTGVLALEL
                     RHGAEQREDVRASVAILAAQMSTLVGVPALQTMSA"
     gene            complement(1907..>2479)
                     /locus_tag="AC16CY_18280"
     CDS             complement(1907..>2479)
                     /locus_tag="AC16CY_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355805.1"
                     /translation="LQTQNGKPTPIFQVTMSLSVGGVGMVKEEVTFVGPPMLSREAAL
                     AVTRVFLVPEDMGTPAGAIALRGATNFQTPSGWDTVYTPILNMIERPVAPMLSVRVET
                     DWYAHESEFRYVLQAGEGITVEPSIPIGQVFFVPREPITMRPCTDEELKAIEESRVEF
                     SRHKAEATQKTSYGLSYSPHYIRQSRAQKE"
BASE COUNT          405 a          850 c          825 g          399 t
ORIGIN      
        1 aaacaccgac tgcatgtagg cgagcacggc cgtcgtcgat ggtgcgtgac tcggcgcgcg
       61 cacgatccgg gcggctgcgt gaagcggaat cacgaggagc agcagatgcg cagctccgat
      121 gggaaccccg ccgagcacga atgccggcac ggacgccacg agctcctcca gcacgaagag
      181 ccacgcggca atgagatggg agaggacgaa gaacaccacg gcccgcgcca cgaactgtgg
      241 ccccttcagt cgctcgagaa gctgccgtcg agggctcatg agcagctgca cgaaatcggc
      301 gggatagttg atcatcgagc gcaggagcaa ggcgcccagc gcaggtgcgg cctccgccga
      361 ctcggcgttc ggggcgacgt acttctcgag ctcgtgaacg agccggtcgc tgtcgtattg
      421 ccaccgctgt tcggtgatct cgtgcgcctg gtgccggagc agctcgcgca tcacgtcggg
      481 cagcgccgag gccgacggca tcgccgctcc ctcgaccagc accggtatga ccgggagccg
      541 ccgttgaagc gccgtgacga cctcgaggcg gacgtagtcc aacggatcgt gcagccgcgg
      601 acggccgtct gagccgatcg cctcgagcca tcgcgggccg atcatcacca ggacgaccgc
      661 gctggccgaa accttcgcgg cgatcgtggc ggcgaagtcg gcgccaggct cgatcgactc
      721 gacgtcgtga aacacctgcg cggcgtcgaa gtgctgctgc agcttgtcgc agagcctgcc
      781 cgtcgcgctc tgggtgtcct gacggcggta gctcacgaag atcaggggca cgcgaggacc
      841 tataatcgtg gatcgacgac tcctacgcta cctgagcgtt agaacgttca tgcgctcccc
      901 caggacccct atgagttgtc gccggccgtt cgagtcggcc tgcgaggtgt gtcatgagcg
      961 cgactttcgg tacccggctt cggttgcagc gcgaacgaca gcagatttcg ctggcgtcga
     1021 ttgccgagca gaccaaaatc aggcttccgc tgctcgaggc gctcgagcgc ggcgatgcgt
     1081 cgaactggcc gcgaggcatt ttcgggcgct cctacgtccg cgcgtacgct cgagccgtcg
     1141 ggctggatcc agacgccacg gttcgagagt tcatcgaatg tcatcccgag ccggacgaat
     1201 cgctgccccc ggttgtcggg gattcgcggg acatcggccc cgacaagctg ggccggcctc
     1261 cgatacggct gcaggtcctg atcgattctg cgatcgacgc ctttcatgcc cgccgcgctg
     1321 aagccgtcga gagaacggca cccacgaccg aaaagaagct gcccgctgcg cccgtcgaca
     1381 tggcacctcc acgccacccg tctccgggca gcggcgccgg gccctccccc gtgaacatgt
     1441 gggccgtggc tgacgtgtgc acgaagctcg ggtgcgcccg ggaggctcac gaactgagat
     1501 ccgcgctcga ggatgcgtcg ggggtcctgg acgccgccgg cctgattctc tggatgccgg
     1561 atgcccgccg agccacgctg acgcccgtgt tcgcgcacgg ctattcagac gacgtgctcg
     1621 cgcgcctccc cgtccttcat acacacgatg acaacgcgac cgctgccgcg ttcaggacgc
     1681 acaccacgtg cgtcgtcaac gcgagccact cccggcacgg cgcggtggtg gcgccgctgc
     1741 tgacgcccaa cggctgcacc ggcgtgctcg cgctggagct gcggcatggc gcggagcagc
     1801 gggaagacgt tcgagcctcg gtggcgatcc tcgccgcgca gatgtcgacg ctggtcggcg
     1861 tgccggcgct ccagacgatg agcgcctgat cggcggtagg cgtcgcctac tctttctgcg
     1921 cgcggctctg ccggatgtag tgagggctgt agctgagccc gtacgaggtc ttctgcgtgg
     1981 cctcggcctt gtgccggctg aactccactc gcgactcctc gatcgccttc agctcctcgt
     2041 ccgtgcacgg gcgcatcgtg atcggctctc gcggcacgaa gaacacctgt ccgatcggga
     2101 tgctgggctc gaccgtgatc ccctcgccgg cctgcagcac gtagcggaac tcgctctcgt
     2161 gcgcgtacca gtccgtttcg acgcgcacgc tcagcatcgg cgccacgggg cgctcgatca
     2221 tgttgaggat cggcgtgtac accgtgtccc agcccgacgg cgtctggaag ttcgtggcgc
     2281 cgcgcagcgc gatcgcaccg gccggcgtgc ccatgtcctc gggaacgagg aagacgcgcg
     2341 tcacggcgag cgccgcctcg cgcgacagca tcggcgggcc gacgaaagtc acctcctcct
     2401 tcaccatccc gacgccgcca accgacaggc tcatcgtgac ctggaaaata ggcgtcggct
     2461 tcccgttctg cgtctggag
//
LOCUS       DBNGSH010000797         5283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1956681, whole genome shotgun sequence.
ACCESSION   DBNGSH010000797 DBNGSH010000000
VERSION     DBNGSH010000797.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1956681"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="AC16CY_18285"
     CDS             <1..320
                     /locus_tag="AC16CY_18285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3355806.1"
                     /translation="WDAKFAYFNPRPSQMDPELKTIIGLPNFPSYTSGHSTFSSAAAE
                     VLSYLFPGDAAYFEAQKEEASISRLYGGIHYRSDIEVGKSHGKRIGGYTVSFARTDGA
                     DFP"
     gene            complement(359..1183)
                     /locus_tag="AC16CY_18290"
     CDS             complement(359..1183)
                     /locus_tag="AC16CY_18290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3355807.1"
                     /translation="MMAAAEPDVRQQEQALMRIDGARALVTGASSGIGAATARALAAR
                     GASVVLIARNIERLNAVVAGIEANGGHAKAYAADLAVAHEVNRVARLVCEHHGVPDIV
                     VNNAGAGRWLPIVDTSADELAAMVAVPFLAAFNLTRELLPGMRGRGSGHIVNVTSIGS
                     FLVWPGAAGYLSARWAMRGFNEALRADVYGSGIGVSLVGFAQVSSAFWEHNPGSWERL
                     PAAAHRLRTLTPDDAAASIVEAVEKEKRLVLRPRMIYLLRLLNTLLPRGTERQMWQ"
     gene            complement(1214..1921)
                     /locus_tag="AC16CY_18295"
     CDS             complement(1214..1921)
                     /locus_tag="AC16CY_18295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR3355808.1"
                     /translation="MFDASAEFYDLIYATIKDYPAEAGHIASLLRRLNPACRTVLDVA
                     CGTGEHARLLAHAGFIVDGVDLDAVFVRLARQKHPSGRFFEADMATFRLPHRYDAVTC
                     LFGSIGYLRTLDRVGEALTSFRGHLAPGGVVVIEPWFAPGVLEHGRVAHNTGEVGGIR
                     VTRHSRVDVAGRLSRLHFDYEIDDGSGTRRASEVHELGLFTTAELLEAFRRAGLEAHY
                     DPKGFTDRGLYLARIAA"
     gene            2093..4216
                     /locus_tag="AC16CY_18300"
     CDS             2093..4216
                     /locus_tag="AC16CY_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl esterase domain-containing protein"
                     /protein_id="HIR3355809.1"
                     /translation="MHRAAWIAIVIGASLLGSASSLAQGAQSGSLPEPKNWTTAQDHQ
                     DMMAQLGITRLRPGPSGNESAPNAANYDEAKANPFPNLPPVLTLKNGKPVTTAAAWWN
                     ERRPEIVEDFEREVVGRVPRVVPTVTWTVTETLQQTVGGRPVVAKQLAGRVDNSSYPA
                     IAVEIQMTLVVPAEAKGRVPVMMMFGRGVLTPPPARQGGPPPPSAAPSDPPATEQLIA
                     AGWGYASISPTSVQADNGAGLTRGIIGLVNKGQPRKPDDWGSLRAWAWGASRGLDYLE
                     TDAAVDATRVGIEGVSRYGKAALVAMAFDTRFASSLVGSSGEGGAKLHRRNWGEQVEN
                     LTGAGEYHWMAGNFMKYGAAEATFGSRNAGDIPVDAHQLIALCAPRATFISYGVPERG
                     DARWLDQQGSFMAAVAAGPVFRLLGARDLGTSDDYKTEKMPAVNVSLLDGELAWRQHD
                     GGHTDGPNWKHFIPWADRLLSKGRQPVRRSDRNSAVAHEQLLAKARQGRVDVYFSGDS
                     ITRRWGATDYPDLLAHWTSNFFGWNAGNFGWGADTTQNILWRLSNGELDGVNPKVIVL
                     LAGTNDIGRTVADDADGKAAEVARGIAAVLDVMRQKAPAATIVLTGIFPRNDNIGVMP
                     TIDKVNRRLSQLADGRGVRYLNVNDKLADSAGRLHDGMMGDRIHPTVKGYQVWADALK
                     PVLTEILGPRAKTDLAPPPIGDPSA"
     gene            4415..>5283
                     /locus_tag="AC16CY_18305"
     CDS             4415..>5283
                     /locus_tag="AC16CY_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3355810.1"
                     /translation="MTTRKVVVGVVVAVFVAAVISVSVQGQSAPTRFITGVVQGAAGP
                     EAGVWVIAETKDLPTNFIKIVVTDDRGRFMLPELPAASYSVFVRGYGLADSARQQMKP
                     GTAPVTLRVNPAKTPLEAAQVYPGNYWLSMLEPPAASEFPGTGAKGNGLGPTMLSQNH
                     WINSLKSDCNFCHQLGNQLTRSVDHVLKAKPELKTHAEAWEWRLGTGVRGDSMYGVIT
                     RQGPDRSLKVYADWTERVAKGEVPPAPPRPSGVERNLVLTLWDVGDDHSFMHDEVATD
                     KNRPTVNANGPIYA"
BASE COUNT          961 a         1740 c         1728 g          854 t
ORIGIN      
        1 gttgggacgc gaagttcgcc tacttcaacc cgcgcccctc gcagatggat ccggagctca
       61 agacgatcat cggcctcccg aactttccgt catacacgtc ggggcactcg accttctcga
      121 gcgcggcagc cgaagtcctg tcctacctgt ttcccgggga cgccgcgtac ttcgaggcgc
      181 agaaggagga ggcctcgatc tcgcggctgt acggcggcat tcattatcga agcgacatcg
      241 aggtcggcaa gtcgcacggc aagcggatcg gcggctacac ggtcagcttt gccaggaccg
      301 acggcgccga ctttccctga tcgcgttccc gggcatggac caatcagggc gcacccactc
      361 attgccacat ctggcgttcg gtgcctcgag gcaacagcgt gttcagcagt cgaagcaggt
      421 agatcatccg cggccgtagc accagtcgtt tctctttttc aaccgcttcg acaatcgaag
      481 ctgccgcgtc gtcaggcgtc aacgtgcgca accgatgtgc agcggccggg agccgttccc
      541 agcttcccgg gttgtgttcc cagaaggcgc tgctgacttg ggcgaatccg acgaggctca
      601 cgccgatgcc tgacccgtac acgtcagccc gcagagcttc attgaaacct ctcatggccc
      661 atcgtgccga gaggtatccc gcagcgccgg gccagaccag aaacgacccg atcgaggtca
      721 cgttcacaat gtggcccgaa ccgcgtccgc gcatgcctgg caagagctcg cgagtgaggt
      781 tgaatgcggc caggaatgga acggccacca tcgcagccaa ctcgtcagcg ctcgtgtcga
      841 cgatgggtag ccagcgtcct gcgccggcgt tgttgacgac gatgtctgga acaccgtgat
      901 gctcgcacac gagtcgagcc acacgattca cctcgtgagc cacggcgagg tcggcggcat
      961 atgccttggc gtgaccgccg ttggcttcga tgccagccac gaccgcattg aggcgttcga
     1021 tattccgtgc aatcaaaacg actgacgccc cacgcgccgc caaggctcgt gcagtcgccg
     1081 cgccaatgcc actcgatgca cctgtgacga gggcgcgcgc gccgtcgatt ctcatgaggg
     1141 cttgctcctg ctgcctgacg tcaggctcgg cggcggccat cattgcaccg gccgcggccg
     1201 gctgcaaacc gggtcaggca gcgattcgag ccaggtagag cccgcgatcc gtgaacccct
     1261 tgggatcgta gtgtgcctcc agccctgctc gtcgaaatgc ctccagcaac tctgcggtag
     1321 taaagaggcc gagttcatga acctcacttg cgcgtcgtgt tccgcttccg tcatcgatct
     1381 cgtagtcgaa gtggagacgc gagagacgtc ccgcaacgtc gacacgactg tggcgcgtca
     1441 cacggatccc gccgacttcg ccagtgttgt gtgcgacccg cccgtgttcg aggaccccgg
     1501 gtgcaaacca gggttcgatg acaaccacgc caccaggcgc caggtgcccg cggaaactgg
     1561 tcaacgcttc gccgacacga tcgagggttc gcaggtagcc gatcgagccg aacaggcatg
     1621 tgacggcatc atatcggtgc gggagtcgga acgtggccat gtcggcttcg aagaaccggc
     1681 cgctcgggtg tttctgccgg gcgaggcgca cgaatacggc gtcgagatcc acgccatcca
     1741 cgatgaagcc cgcgtgagcc aggagccgcg cgtgctcgcc ggtgccacac gccacatcga
     1801 ggactgtcct gcatgccgga ttgagccgcc gtaacagact tgcgatgtga ccagcttcgg
     1861 cggggtagtc tttgatggtc gcgtagatca gatcgtagaa ctcggccgag gcgtcgaaca
     1921 tgatcgtgag tgtctgttgc tgcccagcgt gtggtcggca gagccgcgta aacggttacc
     1981 gtggcgacgg ccgcagtgta gcgccgaaga ggcgattctt gtgcctctct cactcggacc
     2041 gtgccccggg ggcagggtcg tgtatcctga caccggccag gagggtaacc gcatgcaccg
     2101 cgcagcgtgg atagccatcg tcatcggcgc gtcgctcctt ggatccgcgt cgagccttgc
     2161 gcaaggcgcg cagagcggat cgcttccgga gccgaagaac tggacgaccg cccaggacca
     2221 ccaggacatg atggcgcagc tcggcatcac gagactgcgc cccggcccga gcggcaacga
     2281 gtcggcgccg aacgccgcga actacgacga ggccaaggcg aacccgtttc cgaacctgcc
     2341 gcccgttctc acgttgaaga acggcaagcc ggtgacgacc gcggccgcgt ggtggaacga
     2401 gcggcggccc gagattgtcg aggacttcga gcgcgaggtc gtcggacgcg ttccccgcgt
     2461 cgtgccgacg gtgacgtgga ccgtgacgga gacgcttcaa cagaccgtcg gcggccgtcc
     2521 cgtcgtcgcg aagcagcttg ccggccgcgt ggacaacagc tcgtacccgg cgatcgcggt
     2581 cgagatccag atgacgctgg tcgtgcccgc cgaggcgaaa ggccgcgtgc cggtgatgat
     2641 gatgttcgga agaggcgtgc tcactcctcc gcccgcccga caaggcgggc cgccgcctcc
     2701 ctctgccgcc ccgagcgatc cgcccgcgac cgagcagctg atcgcagcgg gatggggtta
     2761 cgcgtcgatc agcccgacga gcgttcaagc cgacaacggc gccggcctca cgcgcggaat
     2821 catcggcctg gtcaacaagg gccagccgcg aaagcccgac gactggggat cgctcagagc
     2881 gtgggcgtgg ggcgcctctc gcggcctcga ctacctcgag accgacgcgg ccgtcgatgc
     2941 gactcgcgtc ggaattgagg gcgtctcgcg gtacggcaag gcggcgctcg tcgccatggc
     3001 cttcgacacg cgattcgcct cgtccctcgt cggatcgtcc ggcgagggcg gcgccaagct
     3061 gcatcgccgc aactggggcg agcaggtgga gaacctcaca ggcgccggcg aatatcactg
     3121 gatggccggc aacttcatga agtacggcgc ggccgaggcg accttcggca gcaggaacgc
     3181 cggcgacatc cccgtggatg cgcatcaact catcgcgttg tgtgcgccgc gcgcgacgtt
     3241 catcagctac ggcgtccccg agaggggaga cgcgcgctgg ctcgatcagc agggcagctt
     3301 catggcggcc gttgcggcgg gacctgtgtt ccggctgctg ggcgccaggg atctcggcac
     3361 ctcggacgac tacaagacgg agaagatgcc cgccgtgaac gtcagcctgc tcgacggcga
     3421 gctcgcctgg cgacagcacg atggcggtca taccgatggc ccgaactgga aacacttcat
     3481 cccgtgggcc gaccgacttc tctcgaaagg acgtcagccc gttcggagat ccgatcggaa
     3541 ttcagccgtc gcgcacgagc agttgctggc caaggccagg caggggcgcg tagacgtgta
     3601 cttttcgggc gactcgatta cccgccgctg gggcgcaacc gattatcccg acctgctcgc
     3661 tcactggacg tcgaacttct tcggatggaa cgcgggcaac ttcggctggg gcgccgacac
     3721 gactcagaac attctctggc ggttgagcaa cggggagctg gacggcgtca acccgaaggt
     3781 gatcgtcctg ctcgcgggca ccaacgacat cggccgtacc gtcgcggacg atgccgacgg
     3841 caaagcggcg gaggtcgcac gcggcatcgc cgccgttctg gacgtgatgc ggcagaaggc
     3901 gcccgcggcc acgatcgtcc tgacgggcat ctttccacgc aacgacaaca tcggcgtgat
     3961 gcccaccatc gacaaagtca accgccggct ctcacagctc gccgacggca gaggagtccg
     4021 gtatctgaac gtgaacgaca aactggcgga cagtgccggc cggctccatg acggcatgat
     4081 gggcgatcgc attcatccga ccgtcaaggg ataccaggtc tgggccgacg ccctgaagcc
     4141 cgtgctcacc gagatactcg ggccgcgcgc gaagacggat ctggctccac cgcccatcgg
     4201 cgacccttct gcctgatggc cagtttgatc tgactccgga tttcgcctct gtcgacaacc
     4261 gtaggggccg gtcttcagac cggcccaggc gggtctgcct tcgactgcgc tcaggccagg
     4321 aaagacccgc ctctacgtcg acaggtattg gctagaatat gtcgcgcgct gccgacggcg
     4381 gcgacgggat gaactctcca gaggagatcg ggacgtgacg acacgaaaag tggtggttgg
     4441 tgtagtagtt gcggtgtttg ttgccgccgt gatctcggtc agcgtgcagg ggcagtccgc
     4501 gcccacccgc ttcatcaccg gcgtggtgca gggcgccgcc gggccggagg caggcgtctg
     4561 ggtcatcgcc gagacgaagg acctcccgac caacttcatc aagatcgtcg tcaccgacga
     4621 tcgcggccgg ttcatgctgc ccgagctgcc ggcagccagc tacagcgtgt tcgtgcgcgg
     4681 ctacggcctc gccgactcgg cgcgccagca gatgaagccg ggcacggccc cggtcacgct
     4741 gcgcgtcaat ccggcgaaga cgccactgga agctgcgcag gtctatcccg gcaactactg
     4801 gctctcgatg ctcgagccgc ccgccgcgag cgagttcccc ggcacgggag cgaagggcaa
     4861 cggcctcggc cccaccatgc tgtcgcagaa ccactggatc aactcgctga agtccgactg
     4921 caatttctgc catcagctcg gcaaccagct cacccgatcg gtcgatcacg tcctcaaggc
     4981 gaagccggag ctgaagacgc acgccgaggc gtgggagtgg cggctcggga cgggcgtgcg
     5041 cggcgactcc atgtatggcg tcatcaccag gcaaggcccg gatcggtcgt tgaaggtcta
     5101 cgcggactgg accgagcgcg tcgcaaaagg cgaggtgcct ccggcgccgc cccgtccgtc
     5161 cggggtcgag cgcaacctcg tgctgacgtt gtgggacgtc ggcgacgatc actcgttcat
     5221 gcacgacgag gtcgcgaccg acaagaaccg gccgaccgtc aacgccaacg ggccgatcta
     5281 tgc
//
LOCUS       DBNGSH010000798         5361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1787049, whole genome shotgun sequence.
ACCESSION   DBNGSH010000798 DBNGSH010000000
VERSION     DBNGSH010000798.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1787049"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2579)
                     /locus_tag="AC16CY_18310"
     CDS             complement(<1..2579)
                     /locus_tag="AC16CY_18310"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355811.1"
                     /translation="MNARDLLLRIRALFRPRRAERELDEELAFHVEREMQKHLANGLS
                     PADARARALARFGSVPLAADQCRDARGTGLIDDLTRDIFYAFRTFRRAPLAALTIVAT
                     VGLGLGMVCVVFMVYNTIYLRTDAVSNPGELFVVERVPGADSSRRVWIPFTQREYDAL
                     RRETGVFTDAVAIVRSVRTRIEGRRVTSSLVSGNFFQVLGVQPVLGRPLMPPDDEASS
                     GKPVIVLSHRGWSKLFPGDSTVIGRSVRVNGRPYEIVGVMPEEFRGLGILPPDYWAPL
                     ALSAQFHDVDSGKEREGIVEVVGRLKPGTSPEAAAAALTIWASAQVDLRTIPGGRLSV
                     RLRPGQGTIVTDLESLLVFSPIFFAFGLILMIGCANVANLLLARGVSRQREIGTRLSL
                     GASRQRVIRQLLTESVLLGLAAAAWGLAVSRFAVESALRAVTSLLPPMLLEVDLTQWA
                     PAIDWRIFVFLAVGAIVSTAFFGLAPALQATRLDLVRTMRGEVANARPARARHALIAV
                     QVGASALLLICSVVFLRGAFDASTVDPGVRTSDTITVAIPNERRRAALLQALAAHTSV
                     AAVAASSRPTLAVAATSGSAEADLSHRMAVDQTPVSPEYFDVLGIDLVRGRSFTQAER
                     SLESGVAIVTETAGRRLWPDRDAVGQIVRLETVPSGVRVTSSVSSADARNARVEQSRA
                     YAIVGVVRDVGGGLRVPDLFNFRGVYVPTGPEQPGTSLTLRVRGDPERVRVTLLDELT
                     SVDPGLGTINTMQSIAGMQMYIMRIFFWVTVVLSGLALLLTLSGLFSVLSYVVAHRAK
                     EIGVRMTLGATTGNVAGLVLSHTARSVGVGLVAGGGLAAALATVLMATPAASEIGGLV
                     RVF"
     gene            complement(2584..2919)
                     /locus_tag="AC16CY_18315"
     CDS             complement(2584..2919)
                     /locus_tag="AC16CY_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3355812.1"
                     /translation="MPRHTIPDLLPGTLDLLILRTLQTSPLHGWAISERIQQISQDVL
                     QVNQGSLYPALHRLEHQGWIEAEWAVSELGRRAKYYRLTPSGRRQLAVEATEWERMST
                     AIGRVMKLA"
     gene            complement(3027..4544)
                     /locus_tag="AC16CY_18320"
     CDS             complement(3027..4544)
                     /locus_tag="AC16CY_18320"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355813.1"
                     /translation="MAKKLALAAICLAFLAGNATAQDAKTVIANAQKALGDPKSVTYS
                     GSAKDVAFQQCGANAAAMVCQGTHNPMRPISNYVRVIDLSAPASRHTGGTNNIGAGGS
                     TAVAAGTFFAQVTSQQADVSGPWAQSVEFYLTPWGFLKGAAANNATATRQGGFTVLTW
                     IPTVKAASGKGYTIHGYVNGKNLIERVETWLGDNIMGDMHIVATYTGWKDFGGAMAPS
                     KIVQTRGGWPFFEVDVTAARTNPSDLASLAPAPAGRGGGPGGAPGGAGRGGPGGPPPA
                     LTVTNEKLGDGLYRLSTGGGSYDSLIVEFRDHVMMLEAGQSPAGGPADARALAYIAET
                     KKLIPNKPIRYIMNSHPHSDHTGGLPAMVAEGATIVTHRNNEAFFEKALNTPRTLLDD
                     TLAKNPKKARVEAVGDKKVYSDGTRTVEMYHVPGAPHSNGLLVAWFPKEKILFQGDFS
                     LPGKQGEPANDHVMALAPVVKKLNLDFERYINVHASAAPQTKAEFWAAVDARTKK"
     gene            complement(4666..>5361)
                     /locus_tag="AC16CY_18325"
     CDS             complement(4666..>5361)
                     /locus_tag="AC16CY_18325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3355814.1"
                     /translation="RLHFDWDPDGACGEFDQALALNPAWVDAHHWHSHALCAAARFPE
                     SLAACRRIVELDPLNPLMHAHVAWHHYMARDFAEALAQAERVVRMEPSFHWGHFFAGW
                     ALERLGRGAEAVAALKEAVRCSAGSPVMLAGLGHALAVLHDRREALRTARDLERLRGG
                     KGLFAYELGVIYAALADADHAFPWLTRALHERSGWMAYLRVDPRLDGLHADPRFDRLV
                     AGAASQSDSKPRN"
BASE COUNT          912 a         1809 c         1807 g          833 t
ORIGIN      
        1 tcgaacacgc ggaccaggcc tccgatctcc gaggcagcgg gtgtggccat caggacggtc
       61 gccagggcgg ctgccagtcc gccgccggcc acgagaccga cgccgaccga ccgcgccgtg
      121 tgtgacagca ccagcccggc aacatttccg gtcgtcgcgc cgagcgtcat gcgcacgccg
      181 atctccttgg cgcggtgcgc gacgacgtac gacagcacgc tgaagaggcc ggacaaggtc
      241 aggagaagcg ccagcccgct gagtacgacc gtcacccaga agaagatccg catgatgtac
      301 atctgcatgc cggcgatcga ctgcatcgtg ttgatcgtgc cgagaccggg atcgacgctc
      361 gtgagctcgt cgagcagtgt cacacgcacg cgttcagggt cgccgcggac gcgaagcgtc
      421 agcgaggtcc cgggctgctc gggaccggtc ggcacgtaca cgcctcggaa attgaacagg
      481 tcgggtactc taagaccgcc ccccacatcg cgcacgacgc cgacaattgc gtacgcacgc
      541 gattgctcca ccctcgcgtt gcgcgcgtcg gcggacgaga ccgacgacgt gacacgcaca
      601 cccgatggca ccgtctcgag gcgtacgatc tggccgacag catcgcgatc cggccagagc
      661 cggcgcccgg cggtctcggt cacgatcgcc actcccgatt ccaggctgcg ttccgcctgc
      721 gtgaagctgc gtcccctcac gagatcgata ccgagcacgt cgaagtactc cggcgatacg
      781 ggcgtctggt ccacagccat tcggtgcgac aggtccgcct cggcggaacc ggacgttgct
      841 gccaccgcaa gcgtcggccg agacgacgcg gcgacggccg caaccgacgt atgggccgcc
      901 agtgcctgga gcagcgcggc gcgccgccgc tcgttgggaa tcgcgacagt gatcgtgtcg
      961 ctcgtgcgca ccccgggatc cacggtcgag gcgtcaaagg ctcctcgcag gaagacgacg
     1021 gagcagatca gaaggagggc cgaggcgccg acctggacgg cgatcagcgc atgccgtgcg
     1081 cgggcggggc gcgcgttcgc cacctctccg cgcatcgtcc gcacgagatc gagccgagtg
     1141 gcttgcagcg cgggcgcaag cccgaagaat gccgtcgaga cgatggcgcc cacagccagg
     1201 aacacgaaga tccgccagtc gatggcaggg gcccactggg tgagatccac ctcgaggagc
     1261 attgggggca gcagcgaggt cacggcacgc agcgcgctct ccacggcgaa acgcgaaacc
     1321 gccaggcccc atgccgccgc tgcgagcccg agaaggacac tctcggtgag aagctgccgg
     1381 atgacgcgtt gacgtgacgc gccgagcgag agccgcgtgc cgatttcccg ctggcgcgac
     1441 acaccccgtg cgagcagcag gttcgcgacg ttggcgcagc cgatcatcag gatcaggccg
     1501 aaagcgaaga agatcgggga gaagacgagc agcgactcca gatcggtcac gatggtgccc
     1561 tggcccggcc tcagcctgac ggagaggcgc cctccgggga tcgtcctgag atcgacttgt
     1621 gctgacgccc agatcgtgag cgcagcggcc gctgcctccg gagacgttcc tggtttcagc
     1681 ctgccgacga cctcaacgat gccttcgcgc tctttcccgc tgtcgacatc gtggaattgc
     1741 gccgagagcg caagcggagc ccagtagtcg ggcggcagga ttcccagccc gcgaaactcc
     1801 tcgggcatga cgccgacgat ctcgtacggc cggccgttga cgcggacgga acggccgatc
     1861 accgtggagt ctcccgggaa cagcttgctc caccccctgt ggctgagcac gatcaccggc
     1921 ttcccggaag aggcctcgtc gtctggcggc atcagggggc gtccaagcac cggctggact
     1981 ccgagtacct gaaagaaatt gcccgagacg agagaggacg tgacccgccg cccctcgatg
     2041 cgtgtccgga ctgaacgcac gatggcgacg gcatcggtga agacgcccgt ctcgcgccgc
     2101 aacgcgtcgt actcgcgctg cgtgaacggg atccagaccc tcctgctgga gtcggcgccc
     2161 ggcacacgct ccacgacgaa cagctcgccg gggttgctca ccgcgtcggt acgaagatag
     2221 atggtgttgt agaccatgaa cacgacgcac accatcccga ggccgaggcc gacggtggcg
     2281 acgatggtga gggccgcgag cggcgcgcgg cggaacgtgc ggaaggcgta gaagatgtcg
     2341 cgcgtgaggt cgtcgatcaa tccggtgccg cgggcgtccc ggcactgatc ggcggcaagc
     2401 ggcacggagc cgaagcgcgc cagcgcgcgg gcgcgagcgt ccgcagggct caggccgttg
     2461 gcgaggtgct tctgcatttc gcgctcgaca tgaaacgcga gctcctcgtc gagctcgcgc
     2521 tcggcacggc ggggacggaa cagcgcgcga atccgcagga gcaggtcgcg ggcgttcatc
     2581 gggtcacgcc agtttcatca cgcgaccgat cgccgtggac atgcgttccc actcggtggc
     2641 ttcgaccgcc agttgcctgc gcccggaggg cgtgagccgg tagtacttcg cccgccggcc
     2701 cagctctgag acggcccact ccgcctcgat ccagccctgg tgctcgaggc gatgcagcgc
     2761 cgggtagagc gagccctggt tgacctgcag cacgtcctgg gagatctgct ggatgcgctc
     2821 cgagatggcc cagccgtgca aggggctggt ttgcagcgtg cggaggatga gcaggtcgag
     2881 cgtgcccggc agcaaatcgg ggatcgtgtg tcgaggcatg gtgtcggata tcgacactaa
     2941 tgctccctgg tgtcgcctgt caacaccagg cacgcggcgg ggcatgacgg acggcggcca
     3001 cgaggaccgc cgtcgtcaca gtgttgctac ttcttcgttc tcgcgtccac cgccgcccag
     3061 aattccgcct tcgtctgcgg cgccgcggag gcatggacgt tgatataccg ctcgaaatcg
     3121 agattgagct tcttgacgac cggcgccagc gccatgacgt ggtcgtttgc cggctcacct
     3181 tgcttaccgg gaagcgagaa gtcgccctgg aacagaatct tctccttcgg gaaccaggcg
     3241 acgagcaggc cgttcgagtg cggagccccg gggacgtggt acatctccac tgtccgcgtg
     3301 ccgtccgagt agaccttctt gtcccctacg gcctcgaccc gcgccttctt cgggttcttc
     3361 gcgagcgtgt cgtcgagcag cgttcgcggc gtgttgagcg ccttctcgaa gaacgcctcg
     3421 ttgttcctgt gcgtgacgat cgtcgcgccc tcggccacca tcgccggcag cccgccggtg
     3481 tgatcggagt gcgggtgcga gttcatgatg tagcggatcg gcttgttcgg gatgagcttc
     3541 ttcgtttcgg cgatatacgc gagagcacgc gcgtcggccg gcccaccggc cggcgactgt
     3601 ccagcctcga gcatcatgac gtggtctctg aactccacga tcagcgagtc gtaactgccg
     3661 cctcccgtgc tcagcctgta caacccgtcg ccgagctttt cgttcgtcac cgtcaatgct
     3721 ggcggggggc cgcccggacc gccacggccc gcgccgcccg gtgcgccacc aggacctccg
     3781 ccgcggcctg ccggtgcagg cgcgagcgat gcgaggtcgg aaggattcgt cctcgcggcc
     3841 gtgacgtcca cttcgaagaa gggccacccg ccgcgggtct gcacgatctt cgacggcgcc
     3901 atcgctccgc cgaagtcctt ccagcccgtg taggtggcga cgatgtgcat gtcgcccatg
     3961 atgttgtcac cgagccacgt ctcgacgcgc tcgatcaggt tcttgccgtt cacgtagccg
     4021 tggatcgtgt agccctttcc ggatgccgcc ttcacggtgg gaatccaggt caggacggtg
     4081 aagcctccct ggcgggtcgc cgtcgcgttg ttggcggcgg cgcccttcag gaagccccac
     4141 ggcgtcaagt agaactccac cgattgcgcc cacggccccg acacatccgc ctgctgcgac
     4201 gtgacctgag cgaagaacgt tccggccgca accgccgtcg agccgccggc gccgatgttg
     4261 ttggtccccc ccgtgtgacg cgatgcgggc gccgagagat cgatgacgcg gacgtagttg
     4321 ctgatcggcc gcatcggatt gtgcgtcccc tggcagacca tcgcggcggc gttggcgccg
     4381 cactgctgga acgccacgtc cttcgcggat cccgaatagg tgaccgactt cggatcgccc
     4441 agcgccttct gcgcgttggc tatcacggtc tttgcgtcct gcgccgtcgc gttcccggcc
     4501 aggaacgcga ggcagattgc ggctagcgcc aatttcttcg ccacggcgac tcctccttca
     4561 cggtcaaggt aaaaggacat cgaactcggt gctcacgagc ggaatgcgga ttgtacagcg
     4621 atcggggaga ctcacgtagt gtccggcttc agccggaccg ttcggtcaat tcctgggctt
     4681 gctatccgac tgagaagcgg cgccggcgac gaggcgatcg aagcgcgggt cggcgtggag
     4741 accatcgagg cgcgggtcga cccgaaggta ggccatccag ccggaccgct cgtgcagcgc
     4801 acgtgtcaac cacggaaagg cgtggtccgc gtcggccagc gccgcgtaaa tgacgccgag
     4861 ctcgtacgcg aacaacccct taccgccgcg cagccgctcg agatcgcgcg cggtgcgcag
     4921 cgcctcccgc cgatcgtgaa ggacggccag tgcgtggccg aggccggcca gcatcaccgg
     4981 gctgcccgcc gaacagcgca ccgcctcctt caaggcggca accgcctcgg ctccgcggcc
     5041 aaggcgctcg agcgcccatc cggcaaagaa gtgaccccag tgaaaagaag gctccatgcg
     5101 cacgacgcgc tctgcctgcg ccagggcctc ggcaaagtcg cgcgccatgt agtggtgcca
     5161 tgcgacgtgg gcgtgcatca gcgggttgag cgggtcgagc tcgacgatcc ggcggcacgc
     5221 tgcgagcgac tccgggaatc gggcggcggc gcagagcgcg tgcgaatgcc agtggtgcgc
     5281 atccacccag gccgggttca acgcgagggc ctggtcgaat tcaccgcacg cgccatccgg
     5341 gtcccagtcg aagtgcagcc t
//
LOCUS       DBNGSH010000799         2519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_768711, whole genome shotgun sequence.
ACCESSION   DBNGSH010000799 DBNGSH010000000
VERSION     DBNGSH010000799.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_768711"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="AC16CY_18330"
     CDS             <1..1305
                     /locus_tag="AC16CY_18330"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3355815.1"
                     /translation="VIVNFFSETVLVGFKCGVALFLASTQLPKLFGFSAGHGGDFWER
                     MGHFLGGLGNTNPTSLALGAGALALLLLGKTFLKNRPVALFVVILGIVAAGVFRLDER
                     GVALLGEIPQGLPMPSLPAVSRADVNTILPLGMACFILAAVETTAIGRMFGAKHGYRL
                     DATQEFLAIGAANLASGLGSAFPVSGGMSQSLVNESGGARTPLSGAVAAIITLLVALF
                     FTGLLRDLPQPVLAAVILVAVTGLIQVDALRHIWRFSRTEFAVAIAALLGVLGSGLLN
                     GVLLGVVISILLLLGRASRPRVTELGRVPGTTYFADLTRHPENERVADVLIVRSEGAL
                     LYFNVDHVRERLSAIVSERAQPPRLVVFFMGNVPHVDLAGVEFLIDLRETYRRSGTEF
                     RLAEVHGQVREALRRSGHASLSELAEANQTVDDLVGKWTKGQ"
     gene            1305..2480
                     /locus_tag="AC16CY_18335"
     CDS             1305..2480
                     /locus_tag="AC16CY_18335"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR3355816.1"
                     /translation="MRPCRVIVVHLAVLLCCAAAVHGQTEGVSASVAAPPGQDSKAAE
                     TPSIYDRIWRFREWYRNDSNPVIQRLRFSGRYQHDFATVSADQGDETEWNVRRMRLGF
                     RANLFHTITVHAEADLNPQEADPVYVRLTDAYVEWSAREHVALTFGKQSMPFTLDGAT
                     SSKELLSVDRSNLANNIWFTEEYLPGVTVSGAPAPWVYIVGVYSAGAKNREFGDFSGG
                     VTTLGSIGYDFATSMQAKEALLSVNYVYQNPDPDNTFTKPFQHVVSLNFKYETARWGF
                     RSDLSATSGYGDQSNLLGITAMPFFNVTPKLQVAVRYTFLDSPDPNGLELAAYEDRVV
                     ADRGDRFNELSLGVSYYFYDHQLKLQSSVQFADMRDLAADGGAYSGVSWTTGIRIGW"
BASE COUNT          397 a          845 c          808 g          469 t
ORIGIN      
        1 gtcatcgtca atttcttctc cgagacggtg ctggtcggct tcaagtgcgg cgtcgccctc
       61 tttctggcga gcacgcagct gccgaagctg ttcggcttca gtgccgggca cggcggggac
      121 ttctgggagc gcatgggcca ctttctcggg gggttgggca acaccaaccc cacctcgctc
      181 gcactgggcg cgggcgcgct cgcgttgctg ttgctcggca agaccttcct gaaaaaccgg
      241 cccgtggcgc tgttcgtcgt gatcctcggc atcgtcgccg ccggggtgtt tcggctcgac
      301 gagcgcggcg ttgccctcct cggcgagatt ccgcaaggcc tgccaatgcc gtcgctcccg
      361 gcggtcagcc gcgccgatgt caacacgatc cttccgctgg gcatggcgtg cttcatcctc
      421 gcagcagtcg agaccaccgc catcggccgc atgttcggcg ccaagcatgg ctacaggctc
      481 gatgcgacgc aggagttcct cgcgatcggc gccgccaatc tcgcctcggg cctcggcagc
      541 gcgttccccg tgagcggagg catgtcgcag tcgctggtca acgagtcggg cggcgcgcgg
      601 acgcccctct cgggagccgt cgccgcgatc atcacgctgc tcgtcgccct cttcttcacg
      661 ggcctgctgc gcgacctgcc gcagcccgtg ctggcggccg tcatcctggt ggccgtcacc
      721 ggcctcatcc aggtggatgc gctgcggcat atctggcgct tcagcaggac ggagttcgcc
      781 gtggccatcg cggcgctgct cggcgttctc gggtcgggcc tcctcaacgg cgtgctgctc
      841 ggcgtggtga tctcgatcct tcttctcctc ggacgcgcct cccggccgcg tgtgaccgag
      901 ctcggacggg tgcccgggac gacctacttc gcggatctga cgcgccatcc ggagaacgag
      961 cgagtcgccg acgtgctcat cgtccggagc gaaggtgcgc ttctctactt caacgtggac
     1021 cacgtgcgcg agcgcctgag cgcaatcgtg agcgagcgcg cccagccgcc gcgcctcgtc
     1081 gtcttcttca tgggcaacgt cccccacgtg gacctcgccg gagtggagtt tctgatcgac
     1141 ctgcgtgaga cctaccgccg atccgggacg gaattccgtc tcgccgaagt gcatggacag
     1201 gtccgcgagg cgctgcgacg ctcgggtcat gcctcgctga gcgaactggc cgaggccaac
     1261 cagaccgtgg acgacctggt tggcaagtgg accaaaggtc agtgatgcgg ccctgccgcg
     1321 tgattgtcgt ccaccttgcg gtgcttctct gctgtgccgc ggccgttcac gggcagaccg
     1381 agggcgtgtc ggcatcggtt gcggcccccc ccgggcagga ttcgaaagcg gccgagacgc
     1441 cgtccatcta cgaccggatc tggagattca gggagtggta ccgcaacgac tccaatcccg
     1501 tgattcagcg gctccggttc agcggacggt atcagcacga cttcgcgacg gtgtccgccg
     1561 accagggaga cgagaccgag tggaacgtcc gccgcatgcg actcggcttt cgggccaacc
     1621 tgttccacac gatcaccgtg cacgcagagg ccgacctcaa tccgcaggaa gccgacccgg
     1681 tctacgtgcg cctgaccgat gcctatgtcg aatggagcgc gcgtgagcat gtcgcgttga
     1741 cgttcggcaa gcagagcatg ccgttcaccc tggatggcgc gacgtcgtcc aaggaactgc
     1801 tgtcggtgga ccgaagcaac ctcgccaaca acatctggtt cacggaggag tatctccccg
     1861 gcgtcaccgt ctccggcgct cccgctccgt gggtctacat cgtgggcgtg tattcggcgg
     1921 gggcgaagaa tcgcgagttc ggtgatttca gcggcggcgt cacaacactc ggatccatcg
     1981 ggtacgactt tgcgacatcg atgcaggcga aggaagcgct gctttcggtg aactacgtgt
     2041 accagaatcc ggatcccgac aacacgttca ccaagccgtt ccagcatgtc gtctcgctca
     2101 acttcaagta cgagaccgca cggtggggat tccggtcgga cctgtcggcc acgtcgggct
     2161 acggcgatca aagcaacctc ctgggcatca cggccatgcc gttcttcaac gtgacgccca
     2221 agctgcaggt tgccgtccgc tacaccttcc tcgacagccc ggatccgaac ggcctggagc
     2281 ttgcggcata cgaggatcgc gtcgtcgcgg accgcgggga caggttcaac gagctctccc
     2341 tcggcgtgag ctactacttc tacgaccacc agctcaaact gcagagctcg gtccagttcg
     2401 cagacatgag ggacctcgcc gctgacggcg gagcgtattc aggggtgtcc tggaccacgg
     2461 gcatccgcat cggatggtga cagtgtcgtg ctacgctgtc ccccttttgg gacgagcgt
//
LOCUS       DBNGSH010000800         5479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2974951, whole genome shotgun sequence.
ACCESSION   DBNGSH010000800 DBNGSH010000000
VERSION     DBNGSH010000800.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2974951"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC16CY_18340"
     CDS             complement(<1..244)
                     /locus_tag="AC16CY_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HIR3355817.1"
                     /translation="MKSRLARGALVLALAAGSLWLASSSVPELQAQNGAPVDTFRVVK
                     AYPHDAAAYTQGLIYREGFLFESTGRHGQSTLRRVRL"
     gene            complement(244..945)
                     /locus_tag="AC16CY_18345"
     CDS             complement(244..945)
                     /locus_tag="AC16CY_18345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3355818.1"
                     /translation="MSRRLRLLGVACAVAFGCASGPTEPSAASAACDPESTSANAVLP
                     ILGRPFDGAFPIGNFFDHDKPIDSDANGYVLTLCGARDTSQVDGHPGYDWRMPEGTPL
                     LAVADAVVLTAGLVAPDFCPQLNRTVQALRVQLVHTAPTGESYVSVYGHLSRVDVQEG
                     VSVSEGTVIGLSGNTGCSGTPHLHFGVARNVAGRYVLIDPYGWHSPMGDPWELDSRGA
                     ASVWLWKGGAAPSLR"
     gene            complement(1075..2043)
                     /locus_tag="AC16CY_18350"
     CDS             complement(1075..2043)
                     /locus_tag="AC16CY_18350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3355819.1"
                     /translation="MNAVRVHTLGGPEVMQDEDIDVPRPGPGEVLVKIAAAGLNYLDV
                     QYRIGRVKAPLPFTIGAEAAGVVSAVGAGVNEFTTGDRVAYSMVLGSYAEYACVPAAK
                     LVAVPASVDLRTAAAAMLQGLTAHYLTHSTFPLAPGDTALVHAAAGGIGMLLTQVARK
                     LGARVIATAGTESKAELAKQAGAHDVILYSKQDFEAEVRRLTEGRGVDVVYDSVGRDT
                     FEKSLNCLRPRGYLVLFGFSSGLVPPFDPAVLGTKGSLFLTRPGLNQYIATRDELLMR
                     ANTLFSWLEDGSVKVRINHTFPLADAVKAHQALEARQTTGKVIFEV"
     gene            complement(2142..3542)
                     /locus_tag="AC16CY_18355"
     CDS             complement(2142..3542)
                     /locus_tag="AC16CY_18355"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR3355820.1"
                     /translation="MPRACFLLPLLLIAGNAAAQPQAQNRPNVVLIITDDVGYGDIGS
                     YGAPDVKTPNIDSLAKAGTRLTDFYAAPNCSPTRAMLISGRYQQRYRIENPLGAARTA
                     GEQGLPATGRTLPRLLKNNGYRTGLVGKWHLGYKPEFSPNAHGFDYFFGFKSGLIDYY
                     QHTDSTGEHDLFENAAPTHVTGYSTDLFAERSVKFIEENAGGPFFLEVAFNAAHWPFQ
                     VPDQPSVAPNNARFVQPQDDPTNTRKDYVAILERADQGIGKILATLDRLGLARNTLVI
                     YTQDNGGEWLSRNAPLFHRKSTVWEGGIRVPAIFRWPGRIPAGKSSSQVGIMMDLTAT
                     ILAVTNSPVPAEAKLEGTNLFPLLQDGAQRTDRTLFWRITGQARRQRAVRQGDWKLLL
                     DGGNPMLFNLAADVGERNDLASQRLDVVRRLFPLITQWELDVDTESGVANEATAGRGQ
                     PAGGRGRGQPAGGRGQ"
     gene            complement(3556..3948)
                     /locus_tag="AC16CY_18360"
     CDS             complement(3556..3948)
                     /locus_tag="AC16CY_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355821.1"
                     /translation="MLTFIFLVLAGFAALVVLPMLIIGGIIWLITLPFRLFFGILGLV
                     FSLVFGAFGLFFGLVGGIFRVVFGLFGAILGLIFGPILLLLLGVALVGGLIVGLFSLL
                     APLVPVVLLALVGWGIYRISTNRPSPSF"
     gene            complement(4020..4310)
                     /locus_tag="AC16CY_18365"
     CDS             complement(4020..4310)
                     /locus_tag="AC16CY_18365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3355822.1"
                     /translation="MSETDDLAALEEMTPEAPGPRPKAPAIESRFLFVDVAAMRAKQL
                     RRGAIVRLDDPDRMGEAPRVIPHKAERIAMEEVRQGLVLYDVPATTKPADKA"
     gene            complement(4436..4846)
                     /locus_tag="AC16CY_18370"
     CDS             complement(4436..4846)
                     /locus_tag="AC16CY_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3355823.1"
                     /translation="MTITRFDPFRELAVFLRDEGVTSRGDWVPPVDIYEGADGALVIK
                     AELPDMQREDVEVTVENRTLTLRGTKNPPAEVKDDQFRRVERRYGSFSRSFTLPGTVD
                     AGKVSADYKNGVLTVKLPYREEARPRTINVEVAA"
     gene            complement(4895..>5479)
                     /locus_tag="AC16CY_18375"
     CDS             complement(4895..>5479)
                     /locus_tag="AC16CY_18375"
                     /inference="COORDINATES: protein motif:HMM:NF012242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR3355824.1"
                     /translation="SVEVHVLQGERQLSRDNRTLGRFHLVGLPPAPRGVPQVEVSFDI
                     DANGIVNVAAKDVATGKEQKITISGSSGLSKDDVARMVKDAEAHASEDQARRDLIDAR
                     NQADSLAYSVEKTIEENRDRLPAVEVSRVEGAIGALREAAKGDSLDAIKAANADLQKA
                     SHAIAEQLYKQQASGPRPQASGQDEVVEGEVVEA"
BASE COUNT          920 a         1849 c         1774 g          936 t
ORIGIN      
        1 cgagcctgac tcgtcggagc gtcgactgac cgtgacgccc cgtgctttcg aacagaaacc
       61 cctcacgata gatcaggccc tgcgtgtacg cggccgcgtc gtgagggtag gccttcacca
      121 ctcggaaggt gtcgacgggc gcaccgttct gggcctggag ttcaggaaca ctgctggacg
      181 ccagccagag cgagccagct gcaagagcga gcaccagtgc gccgcgtgcc agtcgagatt
      241 tcatcatcga agtgatggcg cggctccgcc tttccagagc caaacgctcg cggcgccgcg
      301 cgagtcgagc tcccacggat cccccatcgg agaatgccac ccgtagggat cgatcaggac
      361 atagcggcct gcgacgttcc gcgcgacgcc gaagtgcaga tgcggtgtgc cgctgcagcc
      421 ggtgttgcct gacagcccga tcaccgtgcc ttcgctgacg gagacgccct cctgcacgtc
      481 gacacgtgag aggtgaccgt agacgctcac gtaagactcg ccggtcggcg ccgtgtggac
      541 caactggacg cgcagcgcct gcaccgtgcg attcaactgc gggcagaagt ccggcgctac
      601 gaggccggcc gtcagcacga cggcgtcggc gacggcgagc agcggtgtgc cttccggcat
      661 ccgccagtca tagcccgggt gtccatccac ctggctcgtg tcgcgcgcgc cgcacaaggt
      721 gaggacgtac ccgttggcgt cgctgtcgat cggcttgtcg tgatcgaaga aattgccgat
      781 cggaaacgcg ccgtcaaatg gccggccgag gatcggcaac acggcgttcg ccgaagtcga
      841 ttccgggtcg cacgccgcgg acgccgccga tggctcggtg gggcctgacg cgcagccgaa
      901 ggccacggcg caggccactc cgagcaggcg gaggcggcgg ctcatggccc ttcagacgcc
      961 ggagactact tgacggtctc gtgtgccggt agcgtctggc agctcccacg cgtccctcaa
     1021 gacgcgggaa agtactgaat ccccgtgatc catccagccc tgtcatctcg accctcacac
     1081 ctcgaatatg actttccccg tcgtctgccg cgcttcgagc gcctgatgcg ccttcacggc
     1141 gtcggcgagc gggaacgtgt gattgatcct gaccttgacc gagccatcct cgagccagct
     1201 gaagagcgtg ttcgcgcgca tcagcagctc gtcacgcgtg gcgatgtact ggttgaggcc
     1261 cgggcgtgtc aggaagagcg agcccttcgt tccgagcacg gccgggtcga acggcggtac
     1321 gagcccgctg gagaacccga acagcacgag gtagccgcgc gggcgcaggc agttcaaact
     1381 cttctcgaac gtgtcgcgcc cgaccgagtc gtagacgacg tccacgcccc ggccttccgt
     1441 cagccggcgc acttccgctt cgaaatcctg tttcgaatag aggatgacgt cgtgggcgcc
     1501 tgcctgcttc gccagctccg ccttcgactc ggtccccgct gtcgcgatga cgcgcgcgcc
     1561 cagcttccgg gccacctgcg tcagcagcat gccgatgcca cccgccgccg cgtgcacgag
     1621 cgccgtgtcg cccggcgcga gcgggaacgt gctgtgtgtc agatagtgcg cggtcaggcc
     1681 ttgcagcatt gccgccgccg ccgtgcgaag gtcaacgctc gctggcactg cgacgagctt
     1741 cgcggcgggc acacacgcgt actcggcgta cgatccaagg accatggagt aggcaacgcg
     1801 atctccagtc gtgaactcgt tcacgccggc gccgacggcg ctgacaacgc ctgcggcctc
     1861 ggcgccgatg gtgaagggca gcggggcctt gacgcggccg atgcggtact ggacatccag
     1921 gtagttcagc ccggccgcgg cgatcttgac gagcacctcg cccgggcccg gccgcggcac
     1981 gtcgatgtct tcgtcctgca tgacctcggg cccgccaagc gtgtgcactc gtaccgcgtt
     2041 cacggtgact cccggtccgg ctgaagccgg acactacgtt ctgtcgattg ctggtccggc
     2101 tgaagccgga cactacttcg gtgctgtgga ctgtgaacta cctactgccc acgccctcct
     2161 gccggctgcc cgcgcccgcg gcctccggct ggctgaccgc gaccggccgt cgcctcgttt
     2221 gccactcctg attcagtgtc gacgtccagc tcccactgcg tgatcaaagg gaacagccgc
     2281 cgcacgacat ccaggcgttg gctggcgaga tcgttgcgct ccccgacgtc ggccgccagg
     2341 ttgaacagca tcgggttgcc gccatcgagc aacagtttcc agtcgccctg acgaaccgcg
     2401 cgctggcggc gcgcctgtcc ggtgatcctc cagaagagcg tccgatccgt gcgctgcgcg
     2461 ccgtcctgca gcagcgggaa caggttggtg ccttcgagct tggcctcggc cggcaccggc
     2521 gaattggtca cggccaggat cgtcgctgtg agatccatca tgatgccgac ctgcgacgag
     2581 gacttgcccg cgggaatccg tccaggccac ctgaagatcg ccggcacgcg gattcctccc
     2641 tcccataccg tgctcttgcg atggaacagc ggtgcgttgc gcgacaacca ctcgccgccg
     2701 ttgtcctgcg tgtagatgac cagcgtgttc cgcgcgagtc ccagccgatc gagcgtcgcg
     2761 agaatcttcc cgatgccttg atccgcccgt tcgagaatcg cgacgtagtc cttgcgcgta
     2821 ttggtcggat cgtcctgcgg ctggacgaat cgcgcgttgt tcggagcgac tgacggctgg
     2881 tcgggtacct ggaacggcca gtgcgccgca ttgaaggcca cctcgaggaa gaagggcccg
     2941 ccggcattct cttcgatgaa cttcaccgaa cgctcggcga agaggtccgt cgagtacccg
     3001 gtgacgtgcg tcggcgcggc gttctcgaag agatcgtgct cgccggtcga gtcggtgtgc
     3061 tggtaatagt cgatgagccc gctcttgaag ccgaagaagt aatcgaaccc atgggcattg
     3121 ggactgaact ccggcttgta gccgaggtgc cacttgccga cgagcccggt tcggtagccg
     3181 ttgttcttca gcagccgcgg gagcgtgcgt ccggtggcgg gcagcccctg ttcccccgcg
     3241 gtgcgcgcgg cgccaagagg attctcgatg cgatagcgct gctgatagcg gccgctgatg
     3301 agcatcgcgc gcgtcggcga acagttcggc gcagcgtaga agtcggtcag gcgcgtgccc
     3361 gccttcgcca ggctgtcgat gttgggtgtc ttgacgtcgg gcgccccata gctcccgatg
     3421 tctccatagc cgacatcgtc agtgatgatg agcacgacgt tgggacgatt ctgcgcctgg
     3481 ggctgggccg ccgcgttgcc ggcgatcagc aggagcggaa ggaggaaaca ggctctcggc
     3541 atgcagccga gagtatcaga acgacgggct cggacggttg gtcgagatgc ggtagattcc
     3601 ccagccgacc aacgcgagga ggacgaccgg cacgagcggg gcgagcagcg agaacagccc
     3661 cacgatgagg ccgccgacca gcgccacgcc aagcaagagg aggagtatcg ggccgaagat
     3721 caggccgagg atggccccaa agaggccgaa cacgaccctg aagatcccgc ccaccagacc
     3781 gaagaacaac ccgaacgcgc cgaacaccag gctgaagacc agccccagta tgccgaagaa
     3841 caagcggaag ggcagcgtaa tcagccagat gatgccaccg atgatcaaca tcggaaggac
     3901 aaccagtgcg gcaaatcccg cgagcacgag aaagatgaag gtcaacatct cgactgctct
     3961 tcgtccctaa tccccaccta cgcggctgga cgggcgaagg ttcccgacag gtgacgagat
     4021 caggccttgt cggccggctt tgtggtcgcc gggacgtcgt acagcaccag gccctgccgc
     4081 acttcttcca tcgcgatccg ttccgccttg tgcggtatga cgcgaggcgc ctcgcccatg
     4141 cgatcgggat cgtccagacg gacgatcgcg ccccgccgca actgctttgc gcgcatggcc
     4201 gcgacatcga caaacagaaa ccggctttcg attgcgggcg ccttcggccg gggacccggt
     4261 gcttccggag tcatctcctc gagggcagca agatcgtctg tttcggacat atggcgaaca
     4321 gcctagcagg gggcggggtg cagggggaag ggggatgggg gaagaggctt ctgcccccta
     4381 tcccctgccc cctgccccct gtcgtgatca ggcagccccc tgccccctgt cgtgatcagg
     4441 cagcgacttc gacgttgatg gtgcgcggcc tggcttcctc gcggtacggc agcttgaccg
     4501 tcagcacgcc gttcttgtaa tccgcgctca ccttgccggc gtccaccgtg ccgggcagcg
     4561 tgaacgaacg gctgaaagag ccgtatcggc gctcgacgcg gcggaactgg tcgtccttca
     4621 cctcggccgg cgggttcttg gtgccgcgca gcgtcagcgt cctgttttcg acggtgacct
     4681 cgacgtcctc gcgctgcatg tcgggaagct ccgccttgat cacgagggcc ccgtcggcac
     4741 cctcgtagat atcgacgggc ggcacccagt cgccgcgtga cgtcacgccc tcgtcccgaa
     4801 ggaacaccgc aagctcacga aacggatcga atcgagtaat agtcatgtgc tcttctcctc
     4861 tggccgcggg ggctgaagcc cccgctctac gtcttcaggc ctccacaacc tcgccctcga
     4921 caacctcgtc ctgccctgag gcctgaggcc tgggccctga ggcctgttgt ttgtaaagct
     4981 gctcggcgat cgcgtgcgac gccttctgca ggtccgcgtt cgcggccttg atcgcgtcga
     5041 gactgtcgcc cttcgcagcc tcgcgaagcg cgccgatagc gccctcgacg cggctgacct
     5101 cgaccgccgg cagccgatcg cggttctctt cgatcgtctt ctccaccgag taggcaagcg
     5161 aatcggcctg gttgcgcgcg tcgatcaggt cccggcgcgc ctggtcctcg gacgcgtgcg
     5221 cttccgcgtc cttcaccatc cgcgcgacgt cgtccttgct cagccccgag gagccgctga
     5281 tcgtgatctt ctgctccttg ccggtcgcaa cgtcctttgc cgccacgttg acgatgccgt
     5341 tggcgtcgat gtcgaaggag acctcgacct gcggcacgcc gcgcggcgcc ggcggaagac
     5401 cgaccaggtg gaaacgcccg agcgtgcggt tgtcgcgcga cagctgacgc tcgccctgga
     5461 gcacgtgcac ctcgacgct
//
LOCUS       DBNGSH010000801         6545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3994560, whole genome shotgun sequence.
ACCESSION   DBNGSH010000801 DBNGSH010000000
VERSION     DBNGSH010000801.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3994560"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC16CY_18380"
     CDS             <1..187
                     /locus_tag="AC16CY_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355825.1"
                     /translation="ARGYEIETEMLIKLARRGARIARVPVSLHYGGEPSKLRPLRDTT
                     KTCFLAVRYRFFPERFQ"
     gene            184..1092
                     /locus_tag="AC16CY_18385"
     CDS             184..1092
                     /locus_tag="AC16CY_18385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3355826.1"
                     /translation="MTTAASPSPRWHPRGLNNGAIVSATYYGVSYLPPSLTYPIGHAG
                     TWLAFHLMRNGTRALVENFAVMFPELSPRARDELALRTYRSYARDVIDFIRSLRMTPE
                     ATRGLVTRLDTAALDHVMTEGRGAIVLSGHFGNWEIGGVLLRRLTPYSLSVVAKSEPS
                     QRVTRLRQEMRKGVGVETIEVRQHVDTALRIRERLQQNHAIGMLLDRHLGKDCVEVSF
                     FGRPTKFLRTPAVMAAFSGAPLVPCFVYRDGGGFAVETGPIIRVATTGDRDANVRDAV
                     QTVATLIERHTRQHPQYWYQFYRVWN"
     gene            complement(1113..2234)
                     /locus_tag="AC16CY_18390"
     CDS             complement(1113..2234)
                     /locus_tag="AC16CY_18390"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR3355827.1"
                     /translation="MTRSPGRLTAAIYHRYRTEGDTPVRQALAIGLGLFIGCTPFIGF
                     HSLICIGLGWLFGLNRFKAFAASNISNPLFAPFLYALELQVGAWFRTGQFLSPNMLAE
                     ARLRGLIADVLVGSVVVGSILALAGVLLTYWGSSRRAHPDESALVDAAAERYLLSGIA
                     AWEVARYWMRTDPVYIQVLKDGRLPQRGVLLHLRCGQGLMLALMAAACERWRQDLWPT
                     SWPRPPLDLRLHGIDARRRVVRRARTALGGAATIDERDFSQSPIPSCDAIVLIDVLQV
                     LSRATQDHLIAAAAEALPRGGVCIIREADADAGWRFRAAGFRNRFAAIMRGELSRRFC
                     FDGGSGWSARLTQAGFVIDAVTRHDQGMFGNVLIQARKV"
     gene            complement(2253..2705)
                     /locus_tag="AC16CY_18395"
     CDS             complement(2253..2705)
                     /locus_tag="AC16CY_18395"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HIR3355828.1"
                     /translation="MSDVHLDDLVAQLPHRPPMRLVERIVEIVPGQHACATRVAHAAD
                     WYFDGHFPAQPVIPAIALVELLAQTGGLAAATCRNAELRLAGLSDFKFPAAAGPGVLL
                     RATADVVGRVGSLIRITGTVTADGKLIAKGSLTLAEVPSLEPTGSETA"
     gene            2850..4349
                     /locus_tag="AC16CY_18400"
     CDS             2850..4349
                     /locus_tag="AC16CY_18400"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3355829.1"
                     /translation="MTGPITECFDRVSREHRHRVAIYGLTEGLTRTFGDLRDDANALQ
                     RAIASLGLPSRPVIVSNVGNRTGLAPLFLASLGLQGSLVLVDGDTAAREVNDLAELYG
                     TNLIVASAGAGVLSRPGGVSPVDLPCGLSASVRHRQEAPAWRQPGETGALCLKITSGS
                     LGVSKAVVVSEHTLLSDGLHVIEAMDIRSTDVNLGVVPMAHSYGMGNLLLPLILAGSP
                     VVLRDRFVPTQWAEDVVRLGVTTFPGVPVMFDYLRRTPDAAARLAELRMVITAGAPID
                     TETLRHFKERYGVKVHSLYGTSETGSITFDSSAELHEPVSVGWPMPDTAVSLASVPDL
                     AAPEGRVTVQGPAVARRYAFPDPSEDQSSEFTKEGFLTGDVGAFSSDGRLTLTGRVSG
                     FVNVAGRKVHPREVERVIAELAGVGQVWVLGRNDGARGQELVACVERRSPDLTVAEIR
                     RHCVKTLTPYKVPRRIVFADDLPVNARGKTPRLAIEALLDILERGTNGL"
     gene            4393..6333
                     /locus_tag="AC16CY_18405"
     CDS             4393..6333
                     /locus_tag="AC16CY_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3355830.1"
                     /translation="MVITKALEAVVTNAANVAWTVGIAASRRFRVRSFQAPWAPGPLL
                     KSWEKSSPPLGWPRTTDSLCPICVKRARARILSGADDVEILRNSRVGEIKANIVERDG
                     KIQIEKTCPIHGTFTDTLASDAAFQRRIEGLFPGRDLPAVTDKLHNHGSSSIRYGRGA
                     VLTIDLTNRCNMMCDPCFMDANQVGYVHELNLGEVKQLLDDAITVKPKRQMSVQFSGG
                     EPTLSPVFLESIAYAREVGYYSVQAATNGIRFAQDPGFAMKAKDAGLRIAYLQFDGIG
                     EDANSHRKVGNLFDVKLRAIENLSRAGIDIALVCTIVNTVNNDQVGPIIQFAIDNCEK
                     VSFVSFQPVSFTGRDEDIDDDSRARRRYTLSHLAHDVKTQTSAFEPLRDWFPLSAAGA
                     FADLTDLIQGPAAEWGTMKCGCHPNCGIGAALMISKHTREWAPLTRFLAADRLLDDAK
                     TIVDTGCSPGMAKVLSALSLLRNYRPFEAPEGLSLGALIKKFDKQTGGGLGGRLGAPP
                     DGNRKNDEWLLLFVAGMWFQDLWTYDFRRTEMCIIPYATQLGEISFCAYNTGVGWRQV
                     VEKMYQTATVAEWYKVHGKHAVYANPRHGVPLGNQGPPSLHILGSEASLIPLRSLRRP
                     RPAEVPAPATCAATAGGNVAVT"
     gene            6360..>6545
                     /locus_tag="AC16CY_18410"
     CDS             6360..>6545
                     /locus_tag="AC16CY_18410"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3355831.1"
                     /translation="MRLHHAALVVFLTAPAPAAAQDDLGTYEDRVAVENVMTRYVWAV
                     DSLDADGYVAVFTEDAVI"
BASE COUNT         1183 a         2135 c         2147 g         1080 t
ORIGIN      
        1 cgcccgcggt tacgaaatcg aaactgaaat gctcatcaag ctcgcgcgcc gcggcgcgcg
       61 aatcgcgaga gtaccggtca gcctgcatta cggcggagag ccgagcaagc tccggcccct
      121 gcgagacacc acgaagacgt gctttctcgc tgtccgctac cggttctttc cggagcgatt
      181 ccagtgacga cggccgcttc gccgtcgccc cggtggcacc cgcgaggcct gaacaacggg
      241 gccatcgtca gtgcgaccta ctacggggtg tcgtacctgc caccctcgct cacctatcca
      301 atcgggcatg ccggcacgtg gctcgcgttt cacttgatgc gcaacggaac ccgggcgctt
      361 gtcgagaatt tcgcggtcat gttccccgag ctgagtccgc gcgcgaggga tgagctggct
      421 cttcggacgt acagaagcta tgcgcgcgac gtcatcgact tcatccgcag cctcagaatg
      481 acgcctgagg caacgcgcgg cctcgtgacg cggctcgaca cggcagcgct tgatcacgtg
      541 atgacagaag gacgcggcgc catcgtgctg tcgggccact tcggcaactg ggagattggc
      601 ggcgtcctgc tccgtcgcct cacgccgtac tcgctctcag tcgtcgccaa gtccgaaccc
      661 agccagcggg tcacgcgatt gcgccaggag atgcgcaagg gcgtcggcgt cgagacgatc
      721 gaagtgcggc aacatgtcga taccgctctc agaatccgcg aacgactgca gcagaaccac
      781 gccattggca tgctgctcga tcgacacctc gggaaggatt gcgtcgaggt ctcttttttt
      841 ggccggccca cgaagttcct gcgcacgccc gccgtgatgg ccgccttctc gggcgcgcca
      901 ctcgtaccgt gcttcgtgta ccgcgacggc ggcggcttcg ccgtcgagac tggtccgatc
      961 attcgcgtcg cgacgaccgg cgatcgcgac gccaacgttc gcgacgccgt tcagacggtg
     1021 gccacgctca tcgagcgtca tacacggcag catccgcagt actggtatca gttctaccgg
     1081 gtgtggaact gagacgcgcc cctgcgccgg cgtcagacct tccttgcctg tatcagcacg
     1141 ttgccgaaca tgccctgatc gtggcgagtg acagcgtcga tcacgaagcc ggcctgggtc
     1201 agtcgcgctg accagccgct accgccgtcg aaacagaagc gtctggacaa ttccccccgc
     1261 atgatggcgg cgaagcggtt gcggaagccg gccgcccgga acctccagcc tgcatccgcg
     1321 tcggcctcgc gaatgatgca cactcccccg cgaggcaagg cctcggccgc cgccgcgatc
     1381 aggtgatcct gtgtcgctcg cgagagcacc tgcagcacgt cgatgagcac gatcgcgtca
     1441 caagacggaa tgggtgactg cgagaagtca cgttcgtcga tcgtcgcagc gcccccgagt
     1501 gcggtccgcg cgcggcggac gacgcgacgg cgggcgtcaa tgccgtgaag gcgcagatcg
     1561 agcggtgggc gcggccacga ggtcggccag agatcctgcc gccatcgctc gcacgccgcg
     1621 gccatgagcg cgagcatcag cccctgcccg catctgaggt gcagcagaac gccacgttgg
     1681 ggaagacggc cgtccttgag gacctggatg tacacgggat ccgtgcgcat ccagtagcgc
     1741 gcgacctccc aggctgcgat gccggaaaga aggtagcgct cggcggctgc atccacgagc
     1801 gcgctttcgt caggatgggc tcgccggctc gaaccccagt aggtgagcag gacccctgcg
     1861 agggcgagga tcgatccgac gacgacgctg ccgacgagta cgtcggcgat gagaccgcgc
     1921 aatcgtgcct cggcaagcat gttcggcgac aggaactggc ccgtacggaa ccacgcgccg
     1981 acctgcagct cgagtgcata gaggaatgga gcgaacaggg gattcgagat gttcgaggcg
     2041 gcgaaggcct tgaagcgatt caggccgaac aaccaaccga ggccaatgca tatgagggag
     2101 tgaaagccga tgaagggcgt gcacccgatg aatagaccca gcccgatcgc cagggcctgt
     2161 ctgaccggag tgtcgccctc ggtccggtat cggtggtaga tggccgcagt cagccggcct
     2221 ggggacctcg tcatcgcgcg gacagaagaa cgtcaggcag tctcggatcc ggtgggctcc
     2281 agggagggaa cctcggcaag cgtcaagctg cccttggcga tgagtttgcc gtcggcggtg
     2341 acggtgccgg tgattcgaat gagagatccg actcggccga cgacgtccgc cgtcgcccgg
     2401 agcaggactc cgggacctgc ggcggcggga aacttgaaat ccgagagccc ggccagtctc
     2461 agctccgcgt ttctgcacgt cgccgccgcg agacccccgg tctgtgccag cagctcgacg
     2521 agggcgatgg cgggaatcac cggttgtgcc ggaaagtgcc cgtcgaagta ccagtcggcg
     2581 gcgtgcgcca cgcgcgtcgc gcacgcgtgc tggcccggca cgatctcgac gatccgctcg
     2641 accaatcgca tcggtggacg atgcggcagt tgagcaacca ggtcgtccag atgcacgtcg
     2701 gacatggagg ccgattataa gcctgcggtt ccgggcgctc agcaactcga ggcggattcg
     2761 acgtgcggta tgcttgccac gccgcccgtg ccgtgtggag accgatccac acgaatccgg
     2821 ccgcccactg cggactcaac accctcgtca tgaccgggcc cattacggag tgtttcgacc
     2881 gggtgtcacg cgagcaccgg catcgagtgg ccatctacgg actcaccgaa gggctcactc
     2941 gcacgttcgg cgacctccga gacgacgcga atgcactcca gcgggcgatc gcgagcctgg
     3001 ggctgccctc ccgccctgtg atcgtgtcaa acgtcggcaa ccggaccgga ctcgcgccgc
     3061 tcttcctcgc cagcctcggc cttcagggca gcctcgttct cgttgacggc gacaccgcag
     3121 ctcgcgaagt gaacgatctg gccgaactct acggcaccaa tctcatcgtc gcctcggccg
     3181 gagcgggggt gttgtcgagg cccggaggtg tctcgcccgt cgatctgcca tgtggcctca
     3241 gcgcgagcgt ccgccatcgg caggaggcac cggcgtggcg ccaacccggc gagaccggtg
     3301 cgctctgcct gaaaatcacg tcggggtcgt tgggggtgtc gaaagccgtc gtcgtgtcgg
     3361 agcacaccct cctgtccgac ggtctccacg tgatcgaagc gatggacatt cgatcgacgg
     3421 acgtcaacct cggcgtcgta ccgatggccc attcctacgg catggggaac ctactgctgc
     3481 cgctcatcct cgcgggatcg ccggtcgttc tgcgagaccg cttcgtaccg acgcagtggg
     3541 ctgaggacgt cgtccgtctg ggggtgacga cgtttccagg cgtgccggtc atgttcgatt
     3601 atcttcgacg gacgccggac gctgcggcgc ggctcgccga gctgcggatg gtcatcaccg
     3661 caggcgcgcc catagacacg gagactcttc ggcacttcaa ggaacgctac ggcgtgaagg
     3721 tgcactcgct ctacggtacc agcgagaccg gcagcatcac gttcgattcg tccgccgagc
     3781 tgcacgagcc ggtttcagtg ggctggccga tgcccgacac ggccgtgtcc cttgcctccg
     3841 tgccggacct ggcggcgccg gaaggacgag tgaccgtgca ggggccggcc gtggcgcggc
     3901 gctacgcgtt tccagacccg agtgaggacc agtcgtccga gttcacgaag gagggatttc
     3961 tcacgggcga tgtgggcgcg ttttccagcg acgggcggct gacactcacc ggccgggtat
     4021 ccggattcgt gaacgtcgcc ggccgtaaag tgcatccacg cgaggtcgag cgagtgatcg
     4081 ccgaacttgc cggcgtcgga caggtatggg tgcttggcag aaacgacggc gcacgcggac
     4141 aggaactcgt cgcgtgcgtc gaacgtcgat cgcccgacct gacagtggcc gagatccgga
     4201 gacactgcgt caagacgctt acgccgtaca aggtgccgag acggatcgtg ttcgccgatg
     4261 acttgcccgt gaatgctcgg gggaagacgc cgcggctggc gatcgaggcg ctgctcgaca
     4321 tcctggagcg cggaacgaac ggtttataat accgccgctc caggactcat cgagccgagg
     4381 aggctcgcgg gaatggtgat aacgaaggcg ctcgaggcgg tggtcaccaa cgccgcgaac
     4441 gtggcgtgga cggtgggcat cgccgcctct cgccgctttc gcgttcggtc gttccaggcg
     4501 ccctgggccc ccggaccatt gttgaagagt tgggagaaat cgtcgccgcc gcttggctgg
     4561 ccacgaacga cggactcgct ctgcccgatc tgcgtgaaac gagcacgcgc gcgcatcctc
     4621 agcggcgccg acgatgtcga gatcctgcgc aacagcaggg tcggggagat caaagcgaac
     4681 atcgtcgagc gtgacgggaa gatccagatc gagaaaacct gcccgattca cggcaccttc
     4741 acggacacgc tcgcgagcga cgccgcgttc cagcggcgca tcgagggact tttccccggc
     4801 cgcgatttgc cggccgtcac cgataagctc cacaaccacg ggtcgtcgtc gatcagatac
     4861 ggccgcgggg ctgtcctcac gatcgacctg acgaaccgct gcaacatgat gtgcgatccg
     4921 tgcttcatgg acgcgaacca ggtcggctac gtgcacgagc tgaacctggg cgaagtcaag
     4981 cagttgctcg acgacgccat caccgtcaag ccgaagcgac agatgagcgt ccagttctcg
     5041 ggtggcgagc cgaccttgtc gcctgtgttc ctcgagtcga ttgcctacgc ccgcgaggtc
     5101 ggctactaca gcgtccaagc cgcgaccaac gggatccggt tcgcgcagga tccgggtttt
     5161 gcgatgaagg cgaaggacgc gggcctccgc atcgcttact tgcagtttga cggcatcggc
     5221 gaggacgcca acagccatcg gaaggtcggc aatctcttcg acgtgaagct acgagcgatc
     5281 gagaacctgt cgcgcgcggg catcgacatc gcgctcgtgt gcacgatcgt caacacggtc
     5341 aacaacgacc aggtcgggcc gatcatccag ttcgcgatcg acaactgcga gaaggtcagc
     5401 ttcgtctcgt tccaaccagt gtcattcacg ggccgcgacg aagacatcga cgacgacagc
     5461 cgcgcccggc ggcgctacac gctctcgcat ctggcgcacg acgtcaagac gcagacatcg
     5521 gcattcgaac cactgaggga ctggttcccg ctgtccgctg ccggcgcctt cgccgacctg
     5581 acggacctga ttcagggccc cgccgccgag tggggcacga tgaagtgcgg ctgccatccg
     5641 aactgcggca tcggcgcagc gttgatgatc agcaagcaca cacgagagtg ggcgcctctg
     5701 accaggttcc tcgcggccga ccgccttctc gatgacgcca agaccatcgt ggacacgggc
     5761 tgcagtccag gaatggcaaa ggtgctgtcg gcgctgagcc tcctgcggaa ctaccggccg
     5821 ttcgaggcgc cggaggggct ctcgctcggc gcgctcatca agaagttcga caagcaaacg
     5881 ggcggcgggc tcggcggtcg actcggtgcg ccgccggacg gcaaccgcaa gaacgacgag
     5941 tggctcctgc tcttcgttgc cgggatgtgg tttcaggatc tctggaccta cgatttccgg
     6001 cgcaccgaga tgtgcatcat cccttacgcg acgcagttgg gcgagatttc gttctgcgcc
     6061 tacaacaccg gtgtcggctg gaggcaggtc gtcgagaaga tgtaccagac cgcgactgtg
     6121 gctgagtggt acaaagtcca cggcaagcat gccgtctacg ccaacccgcg ccacggcgtc
     6181 ccgctaggca accagggccc gccgtccctc cacatcctgg gaagtgaggc atctctcatt
     6241 ccgttgagaa gcctgcggcg tccgcgtcct gccgaggtgc cggcgcctgc gacgtgtgct
     6301 gccaccgctg gcggcaacgt cgccgtgacg tagtgcccaa acctggggga acgcgagcca
     6361 tgcgactgca tcacgccgcc cttgtggtgt tcctcacggc gcctgcgcct gccgccgcgc
     6421 aggacgatct cggtacctac gaagaccgcg tcgcggtcga aaacgtgatg acgcggtatg
     6481 tgtgggcggt ggattcgctc gacgcggacg gctacgtcgc cgtgttcacc gaagacgccg
     6541 tcatc
//
LOCUS       DBNGSH010000802         8167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1277502, whole genome shotgun sequence.
ACCESSION   DBNGSH010000802 DBNGSH010000000
VERSION     DBNGSH010000802.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1277502"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..532)
                     /locus_tag="AC16CY_18415"
     CDS             complement(56..532)
                     /locus_tag="AC16CY_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PNPOx family protein"
                     /protein_id="HIR3355832.1"
                     /translation="MLARVTITALLVLLVAGCRAREVEKDLAITEVHTGWYDAGIVDG
                     QNKLVPSITLKLRNVSSEPIARVQVNAVFRRVGEAQEWDSHFVRGIGPDGLAPGTSGD
                     PLVLRSERGYLGPQTRLQMLQNKEFVDARVEVFGRHGSRNWVKMGDYTIDRQLLTE"
     gene            complement(586..1410)
                     /locus_tag="AC16CY_18420"
     CDS             complement(586..1410)
                     /locus_tag="AC16CY_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355833.1"
                     /translation="MKRISILCVMAALSVGAVSAAQQTDDAQVRARQRIAKFEGALET
                     AVKFGADGLLRRVRAVTPDPPMLTGEPEVRGFPLDGYGVFFDVEVPALRLPVTWPLRY
                     MTRDTRNLETLAAEARALSAQASRLDAEFQRQFLQFVRRLEAQAQGPQTMRGTVGALS
                     SAQLGVGIPSESASPRTPTVDPRVLDNPNAAYTGAVKDALIDAIIEDSGALTLGPDEW
                     LTVAARDNLPRDPLVPGDSVDFSTLIFRIKGSDLTAFRAGRLTMEEARAKVDVREY"
     gene            complement(1445..2191)
                     /locus_tag="AC16CY_18425"
     CDS             complement(1445..2191)
                     /locus_tag="AC16CY_18425"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3355834.1"
                     /translation="MSESYTCGDAGALVAYLYDECDPEIRESIDAHLTTCVPCAREIE
                     GLGRTRRLLGDWAPPIAELGFQVPAPVLEEKKPWWRAPLPAWAQAAAALLIFGAGLVM
                     GVSRPSTGPASDAPAAQAAAPAPAASRSDLAQLEQRLRAEMTRLRAGATMGQAGAVPV
                     AAQASAGDDAIMRRVEALIAESEKGLRTEFTLRAVEQRRDLEALRRVDLASVSERFGQ
                     SLGQTNAEIRQQREAINFLLVSQQGPPQGR"
     gene            complement(2188..2778)
                     /locus_tag="AC16CY_18430"
     CDS             complement(2188..2778)
                     /locus_tag="AC16CY_18430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3355835.1"
                     /translation="MTRSDEELVARSMSGDVDSFNQLIVRWERPIYALAYRVLGHEED
                     ARDVCQDTFLRAYRALPGFKGQAKFSSWLYRIALNLCRDWIRRRRRAPVTQIPEDTDP
                     AELGASTGPTESIEDLVERREMSALVEQAMRSLSEEQRTAIILKEYHGMTFQEIADMQ
                     GCPLSTVKTRLYQGLSILRRQLERQAQSVPKLVKRI"
     gene            2972..5017
                     /locus_tag="AC16CY_18435"
     CDS             2972..5017
                     /locus_tag="AC16CY_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3355836.1"
                     /translation="MIQLSDLTKAFGDRTLLDHVTWQIGDGERVGLCGPNGAGKTTLL
                     KILAGMEEADSGAVVKPASLTIGYLPQDGLTHSGRTLVEEASVAFQPMLDIKSEMHDI
                     EHRLGDASVPQDEHDGMLSRYSELQDRFRLGDGYSMDLRIATVLRGLGFSPEDGDRPC
                     ETFSGGWQMRIALAKLLLGRPNLLLLDEPTNHLDLEARNWLEEYLSEYPYAVILVSHD
                     RYFLDSVVTRITDLHLRALVDYVGNYSKYVEQRDAMLERLRQSKREQDEEIARVKMFI
                     DRFRYQATKAAQVQSRIKLLEKVVPIEVPPERKRIHFRFPPCAKSGRTVIELKHAAKS
                     YGALTVFGDVNLHIERGDRIALVGPNGTGKSTLMRLLSGEEAPDRGGRTVGHQVVIEY
                     FAQDEATRLDPSLTVYETLASGSPTDMVPAIRNLLGGFLFSGDDVYKKAGVLSGGERT
                     RLAVARMLLRSSNTLLLDEPTNHLDLDSKDVLLEALEDYGGTLVIVSHDRYFVERLAT
                     KIVEIGHGGAIVYPGTYPEFLWHKEAGLKALDTRRRETGAGKPEPKAKSPEPKGKSPK
                     TEARSQKPGARSQEPEREERKRIEAERRKKERAAQTLQKRISDLECRIAEREGQVKEL
                     EAAMGAPGFYEDRDGAKQVVDRHQSLMWEVGDLMAQWEALQEHAAESPATPGAPSES"
     gene            5201..6364
                     /locus_tag="AC16CY_18440"
     CDS             5201..6364
                     /locus_tag="AC16CY_18440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3355837.1"
                     /translation="MTKSRLRVPPPGSPDRAGAAPIRLTHPPQAAATSDAFFRHIVSG
                     MRNGVLAIRRDGRLALINDEAYRIFGVAPHSDDVGRPVSAVLRDHPDVLRVLGGVFDL
                     HLLPNRVEMRLKPSNKVIGYTVTLVRDDSNAVIGAAMFFKDLTRVEQLVERERLRDRL
                     AAVGEMAAVIAHEVKNPLAGIEVMAGLLRRKIPEAPDAQGVLTDIITEAKMANAIVQE
                     VLDFVRPIRLQVEHTTIADAVHGAIQLADTKARRGDVQVSLAIPDGLQIQADQHQLTQ
                     VFTNLLMNAYEAMAGKGAVAISAELVRLEDGTDPRDAVLVQVVDDGPGMAQDVAERVF
                     NPFFTTKPQGSGLGLAIVRKIVDAHDGRIDLRTSAGKGTTIQVTLPVTGGRED"
     gene            6372..7793
                     /locus_tag="AC16CY_18445"
     CDS             6372..7793
                     /locus_tag="AC16CY_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3355838.1"
                     /translation="MGRILIADDHDSLRRGLAQAVAEAGHDVEEAPNGNAAIERLHEG
                     FFDVVVSDLKMGGSTGLEVLKTAKTLHPTSAIILMTAFGSVSTAVEAMKSGAFDYVQK
                     PFEIEEMEVKIEKALEMRRMQNQIDYLRHAQGDIYDFDRIIGSSGALEKVLAVVRKVA
                     KSNTTVLVRGETGTGKELIAGAVHHNSHRAARNFVKVNCAALQENLLESELFGHEKGA
                     FTGADKQRIGRFEQADGGTLFLDEIGDMSANTQAKILRVLQEHEFERLGGTRTIKVDV
                     RLIAATNRDLTTMVEAGQFREDLYYRLNVVTMEMPPLRERKDDIAELANFFIRRFSGE
                     LKKKIVGLDADALKLLMRYQWPGNIRELENAVERAMLLAEGTHIAVDDLRLGDFGPSG
                     TPREGASIVKIPPTGIPLEEVERIALVEALKMSNWVQKDAAELLSISPRVMNYKIKTL
                     GIDFPRSRRAAVQHHEQPTAVNA"
     gene            complement(7881..>8167)
                     /locus_tag="AC16CY_18450"
     CDS             complement(7881..>8167)
                     /locus_tag="AC16CY_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3355839.1"
                     /translation="EPLFVNARGARLTGRSVQRLVARYVSSCCTRFGISPHALRHSFA
                     THLLQRGADLRAIQELLGHVQLSTTQRYTHVNAAQLLDVYRKAHPRARRT"
BASE COUNT         1464 a         2715 c         2671 g         1317 t
ORIGIN      
        1 agggattggg tggggttcgt agtcacccgc ggattatatg ggccttgggg ccttcttact
       61 cagtgaggag ctgccggtcg atcgtgtagt cccccatctt cacccagttg cgggagccat
      121 gcctgccgaa gacctccaca cgggcgtcga cgaactcctt gttttgcagc atctgcaatc
      181 tcgtctgggg cccgaggtag ccgcgctccg accgcagcac cagggggtcg cccgatgtgc
      241 ccggcgccag gccgtcgggg ccgatcccgc gcacgaaatg cgagtcccat tcctgcgcct
      301 cgcccacgcg acggaacacc gcgttgacct gcaccctggc aatgggttcc gacgagacgt
      361 tcctgagctt caaggtgatg ctcggaacca gcttgttctg gccgtccacg atgccggcgt
      421 cgtaccagcc ggtatgcacc tctgtgatcg cgaggtcttt ctcgacttcg cgcgcacggc
      481 aacccgcaac gaggaggacg aggagagcgg tgatggtgac ccgcgccagc atgggcgaat
      541 tgtacactcc actcgatcag acaccggccg agggaaacgg ttccgctagt actcccggac
      601 gtccaccttc gcgcgtgctt cttccatcgt gagccggcct gcgcggaagg cggtgagatc
      661 gctccctttt atccggaaga tgagcgtgct gaagtccacc gagtcgccgg ggacgagcgg
      721 gtctcgaggc aggttgtcgc gggccgccac cgtgagccac tcgtcaggtc cgagcgtcag
      781 cgcgccgctg tcctcgatga tggcgtcgat cagcgcgtct ttcaccgcgc cggtataggc
      841 ggcattgggg ttgtcgagca cgcggggatc gacagtgggg gtccgtgggc ttgcgctttc
      901 cgacgggatg ccgacgccca actgtgcgct cgacagggct ccgaccgtgc ctcgcatcgt
      961 ctgggggccc tgcgcctgtg cctcgagccg gcgcacgaac tggaggaact gccgctggaa
     1021 ctcggcatcc agccgggaag cctgggccga cagtgcccgc gcttcagccg ccagggtctc
     1081 caggttccgc gtatcccgcg tcatgtatcg aagcggccag gtcacgggca gccgaagcgc
     1141 cgggacctcg acgtcgaaga agacgccgta gccatcgaga ggaaacccgc ggacttcggg
     1201 ctccccggtc aacatcggcg gatccggcgt gacggctctc acgcgacgca gcaggccgtc
     1261 cgcgccaaac ttcaccgcag tttcaagcgc gccttcgaat ttggcgatcc gctgccgcgc
     1321 ccgcacctgt gcgtcgtctg tctgctgcgc tgcgctcaca gcacccaccg acaacgccgc
     1381 catcacacaa agaatcgaga ttctcttcat agctcccacc gcgctcgccg ttgcgcgtcc
     1441 cctgttaccg accctgcggc ggaccctgct gcgacaccag aaggaagttg atcgcttcgc
     1501 gctgctggcg gatctcggca ttcgtctggc ccagggactg cccgaagcgc tcgctcactg
     1561 aggccaggtc cacacggcgc aacgcctcga ggtcccgcct ctgttcaacc gctcgaagcg
     1621 tgaactcggt tcgcagccct ttctcgctct cggcgatcag cgcctctact ctccgcatga
     1681 tcgcgtcgtc gcccgccgac gcctgcgcgg caacggggac ggctcctgct tgtcccatcg
     1741 tcgcgccggc tcgcaaccgc gtcatctcgg cgcgcaggcg ctgctccaac tgggcgagat
     1801 cgctgcgcga cgcggccggc gccggtgcag cggcctgcgc ggccggggca tcagaggctg
     1861 gacccgtcga aggtctcgag actcccatca cgaggccggc gccgaaaatc agcagcgccg
     1921 cggcagcctg cgcccacgcg gggagcggcg ccctccacca gggtttcttc tcctcgagaa
     1981 ccggagcggg cacctggaat ccgagctcgg cgatcggagg cgcccaatca ccgagcagtc
     2041 ggcgggtccg gcccagcccc tcgatctcgc gggcgcacgg gacgcacgtt gtcaggtgcg
     2101 cgtcgatcga ctcgcgtatc tcgggatcgc actcgtcgta gagatacgcg accagcgcgc
     2161 cagcatcgcc acaggtatag gactcactca tatccgtttc accagtttcg ggacactctg
     2221 cgcctgccgc tcgagctgcc tccgcaggat cgacaggcct tggtacagtc tcgtcttcac
     2281 cgtgctgagc ggacatccct gcatgtccgc gatttcctga aacgtcatcc cgtggtactc
     2341 cttcaggatg atcgccgtcc gctgctcctc gctgagcgag cgcatcgcct gctcgacgag
     2401 cgcgctcatc tcgcgcctct cgaccagatc ctcgatcgac tcggtcggcc cggtggaggc
     2461 tcccagctcc gcgggatcgg tgtcctcggg aatctgcgtg accggagccc gccgccgccg
     2521 ccgaatccag tcgcggcaca ggttgagcgc aatacgatac aaccacgacg aaaacttcgc
     2581 ctgtcccttg aagccaggga gcgcccggta cgcgcgcagg aacgtatcct ggcagacatc
     2641 acgcgcgtcc tcctcgtgcc cgaggacgcg gtacgcgaga gcgtagatcg gccgctccca
     2701 tctcacgatg agttgattga agctgtcgac gtcgcccgac atggaccggg cgacgagctc
     2761 ctcgtcgctg cgcgtcatca gcaaggcgcc aacggcctgt ctgcctacta agacggcggc
     2821 agccgtcgcg cggccgttac cccgatcata cggcccggga aggagtggcg gcctgcaggt
     2881 cacggcgcgc tctcgcgacg ccataagcca ttctgatcac gtcacttagg ccgatcgaga
     2941 cgacagcgcc ggtatacaat tgagcccttc gatgatccag ctttccgacc tgacaaaggc
     3001 cttcggcgac cggacgctgc tcgatcacgt tacgtggcag atcggggacg gcgaaagggt
     3061 cggcctgtgc gggccgaacg gggccggcaa gacgacgctg ctcaagatcc tggccgggat
     3121 ggaagaagcc gactcgggtg ccgtcgtgaa gcccgcaagc ctgacgatcg gctacctgcc
     3181 gcaggacggc ctcacgcact cgggccggac gctggtcgag gaagccagcg tcgcgtttca
     3241 accgatgctc gacatcaagt cggaaatgca cgacatcgag cacaggcttg gtgacgcgtc
     3301 cgtgccccag gacgagcacg atgggatgtt gtcgcgctac tcggagctgc aggaccgctt
     3361 ccggctgggc gacggctaca gcatggatct gcgcatcgcg accgtgctgc gcgggctcgg
     3421 gttcagcccc gaagatggcg atcggccctg cgagacgttc tcaggcggct ggcagatgcg
     3481 aattgcgctg gccaagctcc tcctcggccg gccgaacctg ctgctcctcg acgagccgac
     3541 gaaccacctg gacctcgagg cgcgcaactg gctcgaagag tacttgagcg agtaccccta
     3601 cgccgtcatc ctggtctccc acgatcgcta tttcctcgat tcggtcgtga cgcggatcac
     3661 cgaccttcac ctccgggcgc tcgtcgacta cgtgggcaac tacagcaagt acgtcgaaca
     3721 gcgcgacgcc atgctcgagc gcctgcggca gtccaagcgc gagcaggacg aggagatcgc
     3781 ccgcgtcaag atgttcatcg atcgctttcg ctaccaggcg acaaaggcgg cgcaggtcca
     3841 gagccgcatc aagctgctcg aaaaagtggt gccgatcgag gtgccacccg aacgcaagcg
     3901 cattcacttc cggtttccgc cgtgcgccaa gagcggacgc accgtcatcg agctcaagca
     3961 cgcagccaag tcgtacggcg cgctcacggt gttcggcgac gtcaacctgc acatcgagcg
     4021 cggcgaccgc atcgcgctgg tcgggccgaa cggcaccggc aagtccacgc tcatgcggtt
     4081 gctgtcgggc gaggaagcgc ccgaccgggg cgggcgcacg gtggggcacc aggtggtcat
     4141 cgagtatttc gcgcaggacg aagccacgcg cctcgacccg tcgttgaccg tctacgaaac
     4201 gctggcgtcg ggatccccca ccgacatggt gccggcgatc cggaacctgc tcggcgggtt
     4261 tctcttctcc ggcgacgatg tctacaagaa ggcgggcgtg ctgtctggcg gagagcggac
     4321 gcgactggcg gtcgcccgca tgctgcttcg gtcgtccaat acgctgctgc tcgacgagcc
     4381 gaccaaccac ctggacctgg attcgaagga cgtgctgctc gaagcgctcg aggattacgg
     4441 cggcacgctc gtcatcgtct cgcacgatcg ctacttcgtc gagaggctgg cgaccaagat
     4501 cgtcgagatc gggcacggcg gggcaatcgt ctatcccggg acgtacccgg aatttctctg
     4561 gcacaaggag gccggcctca aggctctgga caccaggcgc cgggaaaccg gagccgggaa
     4621 accggagccc aaagccaaga gtccagagcc caagggcaag agcccgaaga ccgaagcccg
     4681 gagccagaag ccgggagcca ggagccagga gcccgagaga gaagagcgga agcgcatcga
     4741 ggccgaacgg cgcaagaagg agcgggcggc acagacgctg caaaagcgta tttccgacct
     4801 ggagtgtcgt atcgctgaac gcgagggcca ggtcaaggaa ctcgaagccg ccatgggcgc
     4861 gcccgggttt tacgaggatc gggatggcgc caagcaggtg gttgaccggc atcagtcgct
     4921 tatgtgggag gtgggcgacc tgatggcaca atgggaagcc ctgcaggaac atgcggcgga
     4981 atcccccgct actccaggcg ccccttccga atcgtaagtg cgccccgtcc gcaccaacag
     5041 aaaacgctat aggcttacgg ttctgtaatc gataaatggg gtgccaggca cccaatctgc
     5101 cggatcaggc cgctacccga gaggacgccg aagtgagggc cgggcacgcg ctttgccttt
     5161 ttcagagtgg cttgaggtct gctctgtgga gctccaggta atgactaagt cgcgtctccg
     5221 tgtccccccg ccgggctctc ccgatcgggc cggggcggca ccgatccgtc tgactcaccc
     5281 cccccaggcc gcagcgacct ccgacgcctt tttccggcac atcgtgtccg ggatgcgcaa
     5341 cggcgtgctg gccatcaggc gagatggccg tctcgcgttg atcaacgacg aggcgtaccg
     5401 catcttcggc gtggcgccgc actccgacga cgtcggccgg ccggtgtcgg ccgtgctcag
     5461 ggaccacccg gacgtcctgc gggtgctcgg cggcgtgttc gacctgcacc tgttgcccaa
     5521 tcgtgttgag atgcgcctga agccgtcgaa caaggtcatc ggctacacgg tcacgctggt
     5581 acgcgacgat tcgaacgccg tgatcggcgc ggcgatgttc ttcaaggacc tgacgcgggt
     5641 cgagcagctc gtggagcgcg aacggcttcg cgaccggctg gcggccgtcg gcgagatggc
     5701 cgccgtcatc gcccacgaag tgaagaaccc gctggccggc atcgaggtga tggcgggcct
     5761 gctccggcgg aagatccccg aggcgccgga cgcgcaggga gtcctgaccg acatcatcac
     5821 cgaggccaag atggcgaacg ccatcgtgca ggaggtcctc gatttcgtcc ggccgatccg
     5881 gctgcaggtc gagcacacga cgattgccga cgcggtccac ggggcgattc agctcgccga
     5941 taccaaggcg cgccgcggcg acgtgcaggt gtcgcttgcc atcccggacg gactgcagat
     6001 tcaggccgac cagcaccaat tgacacaggt ctttaccaac ctgctgatga acgcgtacga
     6061 ggcgatggcg ggcaaggggg ccgtggccat ttccgccgag ctcgtcaggc tcgaggacgg
     6121 caccgacccg cgggacgcgg tcctcgttca ggttgtcgac gacggacccg gaatggcgca
     6181 ggacgtcgcg gagcgggtct tcaatccgtt cttcacgacg aagccgcaag gctcggggct
     6241 cggcctggcc atcgtgcgga agatcgtcga cgcgcacgat gggcgaatag acctgcggac
     6301 gtccgcgggc aaaggcacga cgattcaggt gacgcttcca gtaacgggcg gccgcgagga
     6361 ctaggtggtg tatgggacgg attctgatag ctgacgacca cgattcgctt cgccggggac
     6421 tcgcccaggc agtcgccgag gcgggccacg acgtcgaaga ggcgccgaac gggaacgccg
     6481 ccatcgagag gcttcacgaa ggcttcttcg atgtcgtcgt gagcgacctg aagatgggcg
     6541 gcagcacggg cctcgaggtg ctcaagacgg ccaagacgct ccatcccacg tcggccatca
     6601 tcctgatgac cgccttcgga tcggtgtcca cggccgtcga ggcgatgaag tcgggcgcgt
     6661 tcgactacgt gcagaagccg ttcgaaatcg aggagatgga ggtcaagatc gagaaggcgc
     6721 tcgagatgcg ccggatgcag aaccagatcg actacctccg tcacgcgcag ggcgacatct
     6781 acgacttcga ccgcatcatc gggtcgagcg gcgcgctcga gaaggtcctg gccgtcgtcc
     6841 gcaaagtggc caagagcaac accaccgttc tggtccgcgg cgagaccggc accggaaagg
     6901 agctaatcgc gggtgcggtg caccacaact cccaccgggc ggcgcgcaac ttcgtcaagg
     6961 tgaactgcgc cgcgctgcag gagaacctcc tcgaatccga gctcttcggg cacgagaagg
     7021 gcgcattcac cggcgcggac aagcagcgca tcgggcggtt cgagcaggcc gacgggggca
     7081 cgctgttcct cgacgaaatc ggcgacatga gcgcgaacac gcaggcgaag atcctgcgcg
     7141 tgctccagga gcacgagttc gagcgtctcg gcggcacgcg caccatcaag gtcgatgtcc
     7201 ggctgattgc cgccaccaac cgcgatctga cgacgatggt cgaggccggc cagttccgcg
     7261 aggacctcta ctaccggctg aacgtcgtca cgatggaaat gcctccgctg cgcgagcgca
     7321 aggacgacat cgccgagctt gccaacttct tcatccgccg cttctcggga gagctgaaga
     7381 agaagatcgt cgggctcgac gccgacgcct tgaagctgct catgcggtac cagtggccgg
     7441 gcaacatccg cgagctcgaa aacgctgtcg agcgcgcgat gctgctggcc gaagggacgc
     7501 acatcgccgt cgacgacctg cggctggggg acttcggccc gagcggcacg ccgcgcgagg
     7561 gcgcctccat cgtcaagatt ccaccgaccg gcatcccgct cgaggaagtc gagcgcatcg
     7621 cgctcgtcga ggcgctgaaa atgtccaact gggtgcagaa ggacgcggcc gagctgctgt
     7681 ccatcagccc gcgtgtcatg aactacaaga tcaagacgct cgggatcgat ttcccgcgca
     7741 gccgccgggc cgccgtccag catcacgaac agccgacggc cgtgaacgcc tgagcgctga
     7801 cgctgtcgtt cgctcttctc gcttctctct tctcgcttcg tgttcacgtt ccgcgggcct
     7861 gacggcccgc tgagcgtcaa tcacgtcctc ctcgcccttg gatgcgcttt tcggtagacg
     7921 tcgagcaact gcgcggcgtt gacgtgggtg taccgctgcg tcgtggacag ctggacgtgc
     7981 ccgagcagct cctggatcgc gcgcagatcg gcgccgcgct gcaacaggtg cgtggcgaag
     8041 gaatgccgca gcgcgtgcgg gctgatgccg aagcgcgtgc agcaggacga gacgtagcgt
     8101 gcgacgaggc gctgcacgct ccgccccgtg agccgcgcgc cgcgcgcgtt gacgaacagc
     8161 ggctcgc
//
LOCUS       DBNGSH010000803         1503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1787808, whole genome shotgun sequence.
ACCESSION   DBNGSH010000803 DBNGSH010000000
VERSION     DBNGSH010000803.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1787808"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="AC16CY_18455"
     CDS             <1..851
                     /locus_tag="AC16CY_18455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3355840.1"
                     /translation="QRFPWVRLVALKENRGFAGGNNAGAREARGRFLALLNNDAVPEP
                     EWLQSLVSGVNEQDGTVLVTSRIVYMHDPGVIDSAGDGLLTWGGAYKRHHGARVEVAR
                     ESAEVFGVCGAACLMPRPVFEELGGFDEDFFASHEDVDLSYRARLLGYRCRYVSDAVV
                     RHHGSATIGRSSEFAVFHGQRNLEWVYVKNTPATLLLRTLPGHAIYTAAACVHFARMG
                     MLGAFLRAKVAAAAGLMGVLRKRSHVQRTRRVAASAIWPLLEHRWLATKRREKQFDIG
                     LAGDIR"
     gene            848..>1503
                     /locus_tag="AC16CY_18460"
     CDS             848..>1503
                     /locus_tag="AC16CY_18460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3355841.1"
                     /translation="MTPEIAILIVNYNAGDDLRRALESGAGECAGSAWEAVVVDNAST
                     DGSASHVAAFAPNATLVRNTVNVGFGRAVNQAAAATTAPLLLIMNPDCHLAAGAIAAL
                     RAELDAEPSCAVVGPRILDPDGAVQGSARGDPDMLTGLFGRTGRLRGLAPTLPVARRN
                     VAVEDAMRSGQRSVAVDWVSGACMIVRREAFAAVGGFDERYFLYWEDADLCRRLRGRG
                     "
BASE COUNT          216 a          506 c          550 g          231 t
ORIGIN      
        1 ggcagcgctt tccgtgggtc cgcctggtcg cgctgaagga aaaccgcggc ttcgccggtg
       61 gaaacaacgc gggagcgcgg gaagcgcgcg ggcggtttct cgccttgttg aacaacgacg
      121 ccgtgcccga gccggagtgg ctgcagtctc tcgtctccgg cgtcaacgag caggacggca
      181 cggtgctcgt cacctcgcgc atcgtgtaca tgcacgaccc gggcgtgatc gacagcgcgg
      241 gagacggcct gctgacgtgg ggaggcgcgt acaagaggca ccacggcgcg cgcgtcgagg
      301 tggcgcgaga atcggccgag gtgttcggcg tctgcggagc ggcctgcctg atgccgaggc
      361 ccgtcttcga ggagcttggc ggattcgacg aggacttctt cgcctcgcac gaggacgtcg
      421 acctgtccta ccgcgcccgc ctgctcggct accggtgccg ctacgtgtcg gacgcggtgg
      481 tcaggcatca cggcagcgcc acgatcggac gctcgagcga gttcgccgtc ttccacggcc
      541 agcgaaacct cgagtgggtg tacgtcaaga acacgccggc gaccctgctc ctcagaacgc
      601 tgcccggcca cgccatctac accgcggcgg cctgcgtgca cttcgcccgg atgggcatgc
      661 tcggagcgtt ccttcgggca aaggtcgctg cagcagcggg cctgatgggc gtgctgcgga
      721 agcgctctca cgtgcagcgg acccggcgcg tggcggcgtc ggcgatctgg cccctgctcg
      781 agcatcgctg gctcgcgacg aagcggcgcg agaaacagtt cgacatcggc ctggccgggg
      841 acatccggtg acgccggaga tcgccatcct catcgtgaac tacaacgctg gcgacgacct
      901 gagacgggcg ctcgaatcgg gcgccggcga gtgcgcggga tccgcctggg aggctgtcgt
      961 cgtcgacaac gcgtcgaccg acggaagcgc atcccacgta gccgcattcg cgccgaacgc
     1021 cacgctcgtt cgcaacacgg tcaacgtggg cttcgggcgc gcggtgaacc aggcggccgc
     1081 cgcgacgacg gcgccgctgc tcctgatcat gaatcctgac tgccacctgg ccgcgggagc
     1141 cattgcggcg cttcgcgcgg agctggacgc cgagccgtcg tgtgccgtcg tcggcccgcg
     1201 cattctcgat ccggacggcg cggtgcaggg gagcgctcgc ggcgaccccg acatgctcac
     1261 gggcctgttc ggccggaccg gccggcttcg agggctggca cctaccctgc ccgttgcgcg
     1321 ccgcaacgtg gcggtggagg acgccatgag gagcggccag cgcagcgtgg ccgtggactg
     1381 ggtgtcgggc gcctgcatga tcgtgcggcg cgaggcgttt gccgcggtcg gcgggttcga
     1441 cgagcgttac ttcctgtact gggaggatgc cgatctctgc cgccggctcc gcggccgcgg
     1501 ctt
//
LOCUS       DBNGSH010000804         2524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1618297, whole genome shotgun sequence.
ACCESSION   DBNGSH010000804 DBNGSH010000000
VERSION     DBNGSH010000804.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1618297"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="AC16CY_18465"
     CDS             complement(<1..803)
                     /locus_tag="AC16CY_18465"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR3355842.1"
                     /translation="MDPALLALARYSAQILVVVSVAAAAATLLCASVPSIRFTYWRAA
                     GALCLVVPWLPIPLAGAPAWSVEFDITAIDAAEPGAAANPVLVTASTAILWIVAAGIL
                     ARLGWLLVGAYRLRQLRRRSVHADLGADIDLDKLELAPYTEFRWSAELTHPATFGIRR
                     PVVLLPERFSELSAPSRRAVVRHELLHVARCDWLGIVIEEHVRALFWFHPAVWWIVEQ
                     VQLAREQLIDQLVVSGTASKREYMNALMTFADADRFTTLSTAFLRRRHL"
     gene            complement(803..1180)
                     /locus_tag="AC16CY_18470"
     CDS             complement(803..1180)
                     /locus_tag="AC16CY_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3355843.1"
                     /translation="MARRRLPALGAQELAIMKVVWRLGDVSVREVYETLRERRPVAYT
                     TVMTMMNTVEAKGYLKKTLDGRAYRYRPAVPESRVVTAMVREFVERVFDGSAGPLLAH
                     LVSETALSEKEREELRRLIDETS"
     gene            complement(1217..>2524)
                     /locus_tag="AC16CY_18475"
     CDS             complement(1217..>2524)
                     /locus_tag="AC16CY_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3355844.1"
                     /translation="PGLNPAGWKYGIGWEDGPLQKNPHQVFMVSPTHAWYMDGAGAPT
                     VPLPPDVAEIFPVELVLNPHGFLKAAALPGANPKAVWRWELGEMGRDGPEVSPERMRV
                     VSITWGKYRVDATVNRENLLQRVHTWVPSETLGDMNYEHEFTNASYVDAGNGIRFPTD
                     WHSHQGWDDNFGAQAESAGHNAFGGSLKDVRANVCPDPVTAPEPVRSAAFPMRVDVET
                     LADGVYLLGGGTHASVAVEFASFIAVYDAPLSEARSLAVIEEIVRRIPGKPIRFVVNS
                     HQHFDHTGGLRAYNHIGATVVTHWKNFDFLNRDVINYAPRTLRPDMVSLWPPTELAEG
                     YYYETVRENYVISDGARTMQIHYVNPLQHAEGMLMAYLPRERLLFQADLINTFDPLPA
                     RLAGDQRSFVNAVQKLKLDVRQLVPMHGRPVPWASVEAAARQP"
BASE COUNT          418 a          841 c          856 g          409 t
ORIGIN      
        1 ttcagatgcc gccgccgcag aaacgcggtg gacagcgtgg tgaagcgatc tgcgtcggca
       61 aatgtcatca gcgcgttcat gtactcgcgc ttcgatgcgg tgcccgagac gacgagctgg
      121 tcgatcagct gctcacgggc gagctgcacc tgctcgacta tccaccagac ggcaggatgg
      181 aaccagaaca gcgctcggac gtgctcctcg atcacgatcc cgagccagtc gcaccgcgcg
      241 acgtggagaa gctcgtgacg gacgacggcc cgccgggacg gcgcgctcag ttcggagaag
      301 cgctcgggaa gcaggacgac cgggcgccgg atgccgaacg ttgccgggtg tgtcagctcg
      361 gccgaccaac ggaactccgt gtacggcgcg agctcgagct tgtccaggtc gatgtcggcg
      421 ccgagatcgg cgtgaacgct gcgtcggcga agctgccgta accggtacgc gcccacgagc
      481 agccagccca ggcgggccag gatgccggcg gcgacgatcc acagtatggc ggtgctggcc
      541 gtcacgagaa ccggattggc ggcagcccct ggctcggccg cgtcaatcgc agtgatgtcg
      601 aactccaccg accaagccgg tgcgccggca agcgggattg gcagccacgg gacgaccagg
      661 cacaacgcac cggccgctcg ccagtacgtg aagcggatcg agggaaccga ggcacagagc
      721 agagtcgccg cggccgccgc aacggagacc acgaccagga tctgcgcgct gtaccgggcg
      781 agcgcgagga gggcgggatc catcagctcg tctcgtcgat gaggcggcgc agttcctcgc
      841 gttccttctc cgacaaggcg gtctcggaga ccaagtgagc gagcagcggc ccagccgaac
      901 cgtcgaacac gcgctccacg aactcccgca ccatcgcggt gacgacgcgg ctctcgggga
      961 cggccggccg ataccggtac gcgcgaccgt cgagggtctt cttgaggtac cccttggcct
     1021 ccaccgtgtt catcatcgtc atcacggtgg tgtaggcgac ggggcgccgc tcgcgcagcg
     1081 tctcgtagac ctcgcgcacc gagacgtccc cgagtcgcca gacgactttc atgatcgcga
     1141 gttcctgcgc gcccagtgcg gggagacggc ggcgagccat gtgactacta gtcgtatagt
     1201 acttgtggac tagttgtcaa ggctggcgcg cggccgcctc gacgctggcc cacggcacgg
     1261 gccgcccgtg catcggcacc agctgacgca cgtcgagttt cagcttctgc acggcgttga
     1321 cgaagctgcg ctgatcgccg gccagcctgg ccggcagcgg atcgaacgtg ttgatgagat
     1381 ccgcctggaa gagcagccgt tcccgcggca ggtacgccat cagcatgccc tcggcgtgct
     1441 gcagggggtt cacgtaatgg atctgcatcg tgcgggcgcc gtcgctgatg acgtagttct
     1501 cccggacggt ctcgtagtaa tagccctcgg caagctcggt gggcggccag agcgacacca
     1561 tgtcgggccg tagcgtacgc ggcgcgtagt tgatcacgtc gcggttgagg aagtcgaagt
     1621 tcttccagtg cgtcacgacc gtggccccga tgtggttgta ggcccgcagt ccgccggtgt
     1681 ggtcgaagtg ctgatgcgag ttgaccacga accggatcgg tttgcccgga atgcgccgca
     1741 cgatctcctc gatgacggca aggctgcgcg cctcgctgag cggcgcgtcg tagacggcga
     1801 tgaagctcgc gaattcgacg gcgacgcttg cgtgcgtgcc gccgcccagg aggtacacgc
     1861 cgtcggccag cgtctccacg tccacgcgca tcgggaaggc cgccgatcgc accggctcgg
     1921 gcgcggtcac cgggtcgggg cacacgttgg ccctgacgtc cttgagcgat ccgccgaagg
     1981 cgttgtgccc cgcgctctcg gcctgcgcgc caaagttgtc gtcccacccc tggtgcgagt
     2041 gccagtcggt cggaaaccgg atgccgttgc cggcgtccac gtagctggcg ttggtgaact
     2101 cgtgctcgta gttcatgtcg ccgagcgtct cgctcggcac ccacgtgtga acccgttgaa
     2161 gcaggttctc tctgttgacc gtcgcgtcaa cgcggtactt cccccacgtg atcgagacga
     2221 cgcgcatgcg ctcgggcgat acctcggggc cgtcgcgccc catttcgccc agctcccagc
     2281 gccacacggc cttcgggttc gcgccgggca gcgctgcggc cttcaggaag ccgtgcggat
     2341 tcagcacgag ctccacggga aagatttcgg caacgtccgg cggcagcggg acagttggcg
     2401 ctccggcccc gtccatgtac caggcgtgcg tcggactcac catgaagacc tgatgcgggt
     2461 tcttctgcag cggcccgtcc tcccagccga tcccgtactt ccaccctgcc gggttcaacc
     2521 cggg
//
LOCUS       DBNGSH010000805         5994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4166504, whole genome shotgun sequence.
ACCESSION   DBNGSH010000805 DBNGSH010000000
VERSION     DBNGSH010000805.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4166504"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="AC16CY_18480"
     CDS             <1..269
                     /locus_tag="AC16CY_18480"
                     /inference="COORDINATES: protein motif:HMM:NF014696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR3355845.1"
                     /translation="EHTEVVIEQRGQQVVVRQIVGVLARRIVCRTTEGAEVRAGDRFG
                     VMKFGSRMDVFLPVDATIHVGVGDAVVGGVTVVATLGPQDGKAT"
     gene            279..1109
                     /gene="pssA"
                     /locus_tag="AC16CY_18485"
     CDS             279..1109
                     /gene="pssA"
                     /locus_tag="AC16CY_18485"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR3355846.1"
                     /translation="MYAHDEHAERRRLLRPLRTRSDDQARRFRRGVYLLPSMFTMGNM
                     FCGYACIVHAMRGELSTAALFIGFAFVLDMLDGRIARMMGTTSAFGLEFDSLADVISF
                     GVAPAILSFKWGLEPLGQIGWAAGFLFVAAAAVRLARFNIQAGTQDKRYFVGLPSPPA
                     ACVPAATVFAYPAGFQGYAEAWAVLAMVIVPALLMVSTIRYRSFKTFDLRSRRSYPVL
                     FLLALGLAVLASKPSIVLLTMAYTYLASAFIGLAWHRLRRRPSTPAVEHDGPTGDTSL
                     "
     gene            complement(1113..5060)
                     /locus_tag="AC16CY_18490"
     CDS             complement(1113..5060)
                     /locus_tag="AC16CY_18490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3355847.1"
                     /translation="MPWRSLLRIGVGGVGCALLVLIAGWIGQRVTLGADDAAMAARVE
                     TEVRGAFDNMASRLRQMAIEVEDPDDIRGATAGETSPARRLLTKASDVVQRAPVADSA
                     LTIYGGEGPVAWAGRPTELAADRLQGEETWFAVPTVLGLRLVYVRPVLAGGTRLGAIV
                     VERPVMSDLDVSGRRARGLQGPGDESFRFATWIAPVSIQLQYEGEPTSNGARTFAVDS
                     PSGRRLLTATVAPDDLAETRRQWASATRSIAIVVLAATLLLLTGPLIDWRNRARSVRP
                     YVTAVGLACLAIVVARVMLRSASPADWSDAAVFSGVNYASTLLPPPLPPLLTSPFDFL
                     LTAGMGFALVALVLFAAEAWRLNVRRSRRAISDNVASFVLAQVAAGTAVAFVLLGHRA
                     LLRDTITQTTLDLLNFSLHSWDTSRLVLQEGLLLAHAAAMAYAVLVFRVALLQWRLPG
                     RHWRGWVLTVVCWALPIVVLELVVERSASQAMPLMTAGAVAIWLATAAGRLVVRFRHG
                     SQALRLVLLTLTVIVPASAFYPTIVQLAREDKEDFVEAQYAPYVRNLRENVQTQLLQS
                     QSQIDAFPDLLDLVTAPDSAGGDLPTDRAFAVWQTTPLASYLITSSVELYGRDGRLVS
                     RFAFNLPEDLSAAPLSQERSCDWEVYEEVAPFFAEERRVLHAGRAFCAADPKAPPLGS
                     IVVHALPDDYENLPFISSQNPYKEMLLSEGPLRREGIFGPDVEFAVYGWSRRPLYAAG
                     ATPWPLPDDVFSRVEQSREEIWAQLARGQQRFDVYLLNDRGGIYALGFPVVSLLDHLV
                     NLAELTVLAIALYVLLLAANTLFKWLARRELGGSALLRELRASFYRKLFLAFVASVFI
                     PVIALVLVTRNYVAAQMEASIEQEAIRTASEAGRVVEDLAAPQAERRGLGIDDNVMVW
                     VSRLIDQDVNMFAGSRLLATSERNLFASGLLPTRLDADVYHALALRNEAATVTRERIG
                     EFEYLVAAALLNVRELGILTVPLTSRQREIDAQLDALDRRALLAALLFILAGAGLGYS
                     MAERISDPVNRLTRATRRIARGDLDARIAATSSDELRRLVEAFNGMAADLQRQRSELE
                     RTNRLEAWAEMARQVAHEIKNPLTPIQLNAEHLRRVHLDRGEPLSPVLQDCVSTILTQ
                     VKLLRRIASEFSSFASSPSAKPAEVDTAALLHEVIDPYRAGLDGRIGLEINIPPSLPP
                     VFVDRSLISRSLTNIVENALHAMPGAGTLGLSAHVVDGVVRIRISDTGAGMDDEALAR
                     AFEPYFSTKASGTGLGLPIAKRNVELSGGTIAVQSVRDQGTTVEISLPLAGSR"
     gene            5207..>5994
                     /locus_tag="AC16CY_18495"
     CDS             5207..>5994
                     /locus_tag="AC16CY_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355848.1"
                     /translation="MSLPYTLKNALKRGALVAAANWPVAFVQAAADSLFKLLVAAPVI
                     GGVFLVALVVGAEPVELMSLDRRQLATTVASSLLVRPAVLAAWVASLAVVLVGGSLLV
                     FLVKGGTVGVLVRGERDAGPIEHPPLMPEVVARAAAFSIERFIESARALFPRYARLGA
                     ALMIVYLVSGGAYLGLLARWAGGGLAVPALLTAAFVAWITLVNLFYLLTQIVVAADDC
                     SVTTAAGRVLLFLRRETRAVSGVFLVVLALVVLATGASVLATGAL"
BASE COUNT          950 a         1976 c         2072 g          996 t
ORIGIN      
        1 acgagcacac cgaagtcgtg atcgagcagc gcggccagca ggtcgtcgtc cggcagatcg
       61 tcggcgtgct ggctcgccgc atcgtgtgcc gcacgacgga aggcgcggag gtccgggccg
      121 gcgatcgatt cggcgtcatg aagttcggtt cgcgtatgga cgtgtttctg ccggtggacg
      181 ccacgatcca cgtcggggtc ggagacgccg tcgtcggggg agtgacggtc gttgccacgc
      241 tgggcccgca agacggcaag gcgacgtaag gactcacaat gtacgcgcat gacgagcacg
      301 cggaacggcg acggctcctg aggccgctgc gcacgcgctc agacgatcag gcgaggcgct
      361 tccgccgcgg cgtctacctg ctgcccagca tgttcacgat ggggaacatg ttctgcggct
      421 acgcgtgcat cgtccatgcg atgcgcggag agttgtccac cgcggcactc ttcatcggct
      481 ttgcgttcgt gctggacatg ctcgacggcc gaatcgcccg catgatgggc acgacgagcg
      541 ccttcgggct ggagttcgac tcgcttgccg acgtcatttc gttcggcgtc gctcccgcga
      601 tcctctcgtt caaatggggc ctcgagccgc tggggcaaat cggctgggcc gccgggttcc
      661 tgttcgtcgc ggccgcggcc gtccggttgg cgcgcttcaa catccaggct ggaacgcagg
      721 acaagcgcta cttcgtcggg ttgcccagtc cgcctgctgc gtgcgtcccg gctgcgaccg
      781 tcttcgccta tccggcaggc tttcagggtt atgccgaggc gtgggcagtc ctggcgatgg
      841 tgatcgtccc tgcgctcctg atggtgagca cgatccgcta ccgcagtttc aagaccttcg
      901 atctccggtc acgccgcagc tacccggtgc tgtttctgct ggcactcggc cttgccgtgc
      961 tggcatccaa gcccagcatc gtgctgctga cgatggccta tacgtacctg gcatctgcct
     1021 tcatcggcct cgcgtggcat cggctccgcc ggcggccctc gacaccggct gtcgagcacg
     1081 acgggccgac cggcgacacc tccctctgac ggctaccggg agccggcgag cgggagactg
     1141 atttcgacag tcgttccctg atcgcgcacg ctctgcacgg cgatcgttcc gccgctcagc
     1201 tccacgttcc gtttggcaat aggcaggccg aggcccgtgc cgctggcctt ggtggagaag
     1261 tagggttcga acgcccgcgc gagcgcttcg tcgtccattc ccgcgcccgt atccgaaatc
     1321 cggatccgca ccacgccgtc caccacgtgc gccgagagac ccagcgttcc agcgcctggc
     1381 atggcgtgaa gcgcattctc gacgatgttg gtcagcgagc gggagatgag gctccggtca
     1441 acgaagaccg gtgggagcga cggcggaatg ttgatttcca gtccgatacg gccgtcgagg
     1501 cctgcacggt acgggtcgat gacttcgtga aggagagccg cggtgtcgac ctccgccggc
     1561 ttcgcgctcg gcgacgacgc aaagctggag aactcggacg cgattcggcg gagcagcttg
     1621 acctgcgtga ggatcgtcga cacgcagtcc tgcaacaccg gactgagcgg ctcgccgcgg
     1681 tccaggtgga ctcggcgcag gtgttccgcg ttgagctgaa tcggcgtcag cgggttcttg
     1741 atctcgtgcg cgacctggcg cgccatctcc gcccacgctt caagacggtt ggtccgctcg
     1801 agctcgctgc gctggcgctg caggtcagcc gccatgccgt tgaacgcctc gacgagccgg
     1861 cgcagctcgt cggaggaggt ggcggcgatc cgggcgtcga ggtcgccacg cgcgatccgc
     1921 cgggtggcac gcgtcaggcg gttcacggga tccgagatgc gctcggccat cgagtacccg
     1981 aggccggcgc cggccaggat gaagagcaga gcggcgagca gtgcgcgccg gtcaagcgcg
     2041 tcgagctgcg cgtcgatttc ccgctgcctc gaggtcagcg gcacggtcag gatgccgagc
     2101 tcccgcacgt tcaggagcgc ggccgcgacg aggtattcga actcgccgat tcgctcgcga
     2161 gtgaccgtgg cggcctcgtt gcggagcgcc agcgcgtgat acacgtcggc gtccagacgg
     2221 gtcggcagca gtcccgaggc gaacaggttc cgttcgctgg tcgcgaggag ccgcgatccg
     2281 gcgaacatgt tgacgtcctg atcgatcagg cggctcaccc agaccatcac gttgtcgtcg
     2341 atgcccagcc cccgccgctc ggcctgcggc gccgcgaggt cctcgaccac gcggccggcc
     2401 tcggaggccg tccggatcgc ttcctgttcg atgctggcct ccatctgcgc ggccacgtaa
     2461 ttccgcgtga cgaggacgag cgcgatcacc gggatgaaca ccgaggcgac gaacgccagg
     2521 aacagcttcc gatagaagct ggcgcggagc tcccgcagga gcgccgaccc gcccagctcg
     2581 cgtcgcgcga gccacttgaa cagcgtgttc gcggcgagca gcagcacata gagggcgatg
     2641 gccagcacgg tcagctcggc caggttcacc aggtgatcga gcagcgagac gacggggaac
     2701 ccgagagcgt agatgccgcc gcgatcgttg aggaggtaga cgtcgaaacg ctgctgaccg
     2761 cgcgcgagtt gcgcccagat ctcctcgcgg gactgttcga cacgcgagaa gacatcgtcg
     2821 ggaagcggcc acggtgtcgc cccggcggcg tacaacggcc ggcggctcca cccgtacacg
     2881 gcgaactcga cgtcgggccc gaagatgccc tcgcgtcgca gcggcccttc cgacagcagc
     2941 atctccttgt aggggttctg cgacgagatg aacggcaggt tctcgtagtc atccggcagg
     3001 gcgtgcacga cgatggagcc cagtggcggt gctttgggat cggcggcaca aaatgccctt
     3061 ccggcgtgga gtacgcggcg ctcctcggcg aagaacggcg cgacctcctc gtagacttcc
     3121 cagtcgcacg atcgctcctg cgatagaggc gccgcggaca ggtcctcggg caggttgaac
     3181 gcgaaccggc tgacgagccg gccgtcgcgt ccatacagtt ccaccgacga cgtgatcagg
     3241 tagctcgcaa gcggcgtggt ctgccagacg gcgaacgccc ggtcggtcgg caggtcgccg
     3301 cctgccgagt ccggcgccgt cacaaggtcc aggagatcgg ggaacgcgtc gatctggctc
     3361 tggctctgca gaagctgcgt ctggacgttc tccctgaggt tgcggacgta cggagcatac
     3421 tgggcttcga cgaaatcctc cttgtcctct cgcgcgagct gcacgatggt tggatagaac
     3481 gcggaggccg gaacgatcac tgtcagcgtg agcaggacca ggcggagcgc ctgcgatccg
     3541 tgacggaagc ggaccaccag ccggcccgcg gccgtcgcca gccagatagc gacggctccg
     3601 gccgtcatca gcggcatggc ctgcgacgcc gaccgctcga cgacgagctc caacaccacg
     3661 atcggaagcg cccagcagac aaccgtgagg acccagcctc gccagtggcg cccgggcagg
     3721 cgccattgaa gcagcgcgac ccggaatacc aagacggcat acgccattgc cgcggcgtgc
     3781 gccaggagca acccttcctg caggaccagc cgtgaggtgt cccacgagtg gagcgagaag
     3841 ttgagcaggt ccagagttgt ctgcgtgata gtgtcgcgca gcagcgcgcg gtgcccgaga
     3901 aggacgaagg caaccgccgt gcccgctgcc acctgtgcca ggacgaatga cgccacgttg
     3961 tccgagatgg cgcgacgcga gcgcctgacg ttcagccgcc acgcttccgc ggcgaacagc
     4021 accagcgcga cgagggcgaa acccattccg gctgtcaaca ggaagtcgaa aggcgaggtc
     4081 agcagcgggg gcagcggcgg gggcaataac gttgacgcgt agttcacgcc cgaaaacacc
     4141 gcggcgtccg accagtcggc gggagacgcc gagcggagca tcacgcgcgc gacgacgatc
     4201 gccaggcacg cgaggccgac tgccgtgacg tacggccgga ccgaccgggc gcggtttcgc
     4261 cagtcgatca gcgggccagt caggagcagc aacgtcgcgg cgaggaccac gatggcaatc
     4321 gagcgggtcg ccgatgccca ttgtctgcgc gtctccgcga ggtcgtcggg ggcgaccgtc
     4381 gcggtcagta gccggcggcc ggaaggggag tcaaccgcaa acgttcgggc gccgttcgat
     4441 gtcggctcgc cctcgtactg cagctggatc gataccggag cgatccaggt cgcgaagcgg
     4501 aacgattcgt cgcctggtcc ctgcaaccct ctcgcccggc ggccgctgac gtcgaggtcg
     4561 gacatgaccg ggcgctccac gacgatggca ccgaggcgcg tgccgccggc gagcacgggg
     4621 cgaacgtaga cgagacgtaa gccgagaacg gtggggacgg cgaaccaggt ttcctcaccc
     4681 tgaaggcggt ccgcggccag ttcggtcgga cgtccggccc aggccaccgg cccctcgccg
     4741 ccgtagatgg tgagagcgga atcggcaact ggtgcgcgtt gcaccacatc ggaggccttc
     4801 gtcagcagcc gtctggcggg cgacgtttct ccagcagtcg cgcctcggat gtcgtccggg
     4861 tcttcgacct cgatcgccat ctgccgcagc cggctggcca tgttgtcgaa ggcgccgcga
     4921 acctcggttt cgacgcgcgc cgccatcgcg gcgtcgtcgg ctccgagcgt gacgcgctgc
     4981 ccgatccacc ctgcgatgag gacgagcaac gcgcaaccca cgcccccgac gccgatgcgc
     5041 agcagcgaac gccacggcat gctgaccatt ctagtggttt gtcgttgtga tcacgggagc
     5101 gggccgcccg ttggttggcc cgcgaaatcg cggtagaaag gctggcccac gcactacggg
     5161 ttcatttgat acggtccact agaggtacca ttgtgtacgt acctcggtgt ccctccccta
     5221 cacgctcaag aacgcactca agcgcggtgc gcttgtagct gccgcgaact ggccggtcgc
     5281 cttcgtccag gcagccgctg actcgctgtt caagctgctg gtggccgctc cggtgattgg
     5341 gggagtgttc ctcgtcgcgc tcgtggtggg ggccgaaccg gtggagctga tgtcgctcga
     5401 tcggcggcag ctcgcgacga cggtggcttc gtcgttgctg gtccgtccag ccgttctcgc
     5461 cgcgtgggtc gcgtcgctgg ccgtagtgct cgttggcggg tcgcttttgg tattcctcgt
     5521 gaaggggggg accgttggcg ttcttgtgcg cggagagcga gacgccggcc caatcgagca
     5581 ccccccgctc atgccggaag tcgtcgcgcg cgccgcggcg ttttcgatcg agagattcat
     5641 cgagtcggcc cgggcgctgt tccctcgcta cgcgcgtctc ggcgcggcgc tcatgatcgt
     5701 ctatctcgtg tcgggcggcg cgtatctggg tctcctcgct cgttgggccg ggggaggact
     5761 ggcagttccc gccctgctca ccgctgcctt cgtggcctgg atcacgctcg tcaacctgtt
     5821 ttacctcctg acgcagatcg tcgtcgccgc cgacgattgc tcggtgacga cggcagccgg
     5881 gcgcgtgttg ctgtttctgc gtcgggaaac gcgagccgtc tcaggcgtgt ttctcgtggt
     5941 gctggcgctg gtggttctgg cgacgggggc gtccgtcctt gccaccggcg cgct
//
LOCUS       DBNGSH010000806         5896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3487691, whole genome shotgun sequence.
ACCESSION   DBNGSH010000806 DBNGSH010000000
VERSION     DBNGSH010000806.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3487691"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC16CY_18500"
     CDS             <1..272
                     /locus_tag="AC16CY_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl cyclase domain-containing protein"
                     /protein_id="HIR3355849.1"
                     /translation="QRALAEHRQAHGFAPQVRIGVHTSDATKIGGNFAGKGVHEAARI
                     AALAQGGEILASRQTMSPRYRASEPRQVTLKGIAEPMEIVSVQWH"
     gene            306..602
                     /locus_tag="AC16CY_18505"
     CDS             306..602
                     /locus_tag="AC16CY_18505"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3355850.1"
                     /translation="MAFAGKVSDVAPGRLLVIDVEGEPVAVANVDGQLYAFSDTCTHR
                     SCPLSDGELSGTVITCACHGGQFDVTSGKVVGGPPREPIATYPVQVVGDEFRIG"
     gene            619..1047
                     /locus_tag="AC16CY_18510"
     CDS             619..1047
                     /locus_tag="AC16CY_18510"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3355851.1"
                     /translation="MVKTDVLAKVPLFAGLDAEAIEAIAGFGFQKTFQAGELIVEEGR
                     TGNGMFIIVSGRAEVIKGLNTARPRTVATLGAGEPIGEMALLGEWPRTASVRAVETTE
                     CMGIDRWIFLDHLNREPKLALRLLQILALRLANTGERLAE"
     gene            complement(1055..2275)
                     /locus_tag="AC16CY_18515"
     CDS             complement(1055..2275)
                     /locus_tag="AC16CY_18515"
                     /inference="COORDINATES: protein motif:HMM:NF024306.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3355852.1"
                     /translation="MIYLLTEAAEIEHGVMCCYLFAAFSLKSSTDEGVTDAQLRKIRK
                     WSRLLRGVAVEEMLHLGAVCNLLTAIGGAPHLRRPNFPIRMDRRGGFHLRLAPFSLET
                     IEQFIAIEEPDELVGHDGKVERSLPAEVQPRLSDIFASEREYHTQGRLYRGIEDGFVY
                     LAQKYGEKGLFISGPEAQIAAPLHFGSLPQLKPVSDLASALEAINVIVVQGEGASRDT
                     PDSHYNTFKTIWQEYKDTLAEAPEFQPTRPVLTNPYALQPIDQADSTDLNFLDDPDTA
                     ELSNLSDACYELMVQILGRLFVRAEESDDELQRLSRAAETLMIDIVVPLADALTRSPA
                     GPSHPGKTAGISFRLSRGATIPTHKDAARAVFHERLGELGAYCTFLQSRDRAPALLAK
                     TKIALARIAKDLGG"
     gene            complement(2333..2779)
                     /locus_tag="AC16CY_18520"
     CDS             complement(2333..2779)
                     /locus_tag="AC16CY_18520"
                     /inference="COORDINATES: protein motif:HMM:NF018595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HIR3355853.1"
                     /translation="MGQGEGRPPGLLKEYKGSHIVVTWEPAYCIHTENCLRTAPEVFD
                     RKQRPWIRVEAADAERIVQTVLKCPSGALHFRRVDGGAPEPVPTETTVEPQANGPLFV
                     RGNLTFFDVNGEVIRKETRAALCRCGQSSNKPFCDGTHETVGFQAP"
     gene            2922..3404
                     /locus_tag="AC16CY_18525"
     CDS             2922..3404
                     /locus_tag="AC16CY_18525"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3355854.1"
                     /translation="MSVRSILVPTDFSETSEAALHYATEMALTLGARLYLMHVPGKTG
                     EHYEAKYPHGQFETATRERLSSFLTKEELERLRPEYALRVGPPAEEIVHYAEICDIDL
                     IIMGTHGRSGIAHALMGSVAEQVVRAAPCPVLLVRAPKRAAVTQGAAVRVPPVKRMLA
                     "
     gene            3622..4446
                     /locus_tag="AC16CY_18530"
     CDS             3622..4446
                     /locus_tag="AC16CY_18530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355855.1"
                     /translation="MAVRGGEVFGFLGPNGSGKTTTIRLLLGFLHPTAGQGRVLGLDM
                     VRDSVAIRRRLGYLPSGIALYDTLTGEQLLDYLADLSGRPASRRAALCERLELSARTL
                     KRLVRDYSRGMRQKIGIVQALQHDPELAILDEPTEGLDPLMQRAFYEIIDTLKAEGRT
                     IFFSSHVLSEVERVCDRVAIVRSGRLVALEETARLVARRKRNITLRFSGPPPRLEAVS
                     GITNVEQGDGCLTCRLEGNVRPFLAAIADAAVVDLTIEPARLEEAFLEFYDEAPSA"
     gene            4443..5246
                     /locus_tag="AC16CY_18535"
     CDS             4443..5246
                     /locus_tag="AC16CY_18535"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355856.1"
                     /translation="MNAALLRHTWRLQRNRLAIVSIALTVWGFLPPIIYARFGSQFTA
                     LVQSGLLPEQFARVGGGDIFSLSGAIALSFIHPIATILTSVFAVGFSAAAVAGERQRG
                     TLELALARPISRRVLYLSLLAASFGFVAISMAALVAGSVSGATFAGVVKELAFGNLPL
                     LWLNSVLLFGAFAAIGLAASVSFDRLAPAFGTALGIVVAMYVLEVLGSLWPAAEFLQP
                     YSLFHYLKARAILNGLAAPFDMFLLSSVIVIAMVWSLAVFPRRDLAAPS"
     gene            5272..>5896
                     /locus_tag="AC16CY_18540"
     CDS             5272..>5896
                     /locus_tag="AC16CY_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3355857.1"
                     /translation="MITSIPSHARTLALSLGAGMVAAALLFLIASSISIDAQAPRSVQ
                     DGVFSDAQAVRGQALYVQRCAGCHGPALEGAQAPPLMGAAFTTKFRAEPLSALFIQIR
                     YAMPPNAAAEAQLTSEQAADLVAHILKTNGFPAGKTDFAGADGPSSQIGWPSAGAAGG
                     NTAAPATMYRPAGNLNQLMRGVFFPNSNLIFSIQEVEAADLPPPPPAS"
BASE COUNT          954 a         1910 c         1925 g         1107 t
ORIGIN      
        1 tccagagggc gctggcggag caccgccagg cgcatggctt cgcgccgcag gttcgcatcg
       61 gcgttcacac atccgacgcc acgaagatcg gtggcaattt cgccggcaag ggcgtccacg
      121 aggccgcccg aattgcggcg ctggcccagg gcggcgagat tctggccagc cggcagacga
      181 tgagcccgcg ttacagggcg tcagagcccc gccaggtcac cctgaaaggg atcgccgagc
      241 cgatggagat cgtttcagtc caatggcatt gaaagacggc ggggcggttg acggggggag
      301 ggaatatggc gtttgccggt aaggtgagtg acgtggctcc cgggagattg ctggtcatcg
      361 acgtcgaagg agagccggtt gccgtggcaa acgtcgatgg gcagctttac gccttcagtg
      421 acacctgcac tcatcgcagt tgcccgctgt ccgatgggga gctctcgggc acggtcatca
      481 cgtgcgcctg ccacggaggc cagttcgacg tgacgagcgg caaggtcgtc ggcggccctc
      541 cccgtgagcc catcgcgacg taccccgtgc aggtcgttgg cgacgagttt cgcatcggct
      601 agagcaggac gagacgtgat ggtcaagact gacgtgcttg ccaaggtgcc cctctttgcc
      661 ggactggacg ccgaggctat cgaggcaatc gccggctttg gttttcagaa gacgttccag
      721 gccggcgagc tgattgtcga agagggccga acgggcaacg gcatgttcat catcgtgtcc
      781 gggcgcgctg aagtgatcaa agggctcaac acggcgcggc cgcgcacggt cgccaccctc
      841 ggtgccggcg agcccatcgg cgaaatggct ctcttaggag agtggccacg gacggcctcg
      901 gtgcgcgcgg tggaaacaac cgagtgcatg ggtatcgatc ggtggatctt cctggaccat
      961 ctcaacaggg agccgaagct tgcgctccgg ctgctccaga ttctcgcgct gcggctggcc
     1021 aacaccggcg agcggctggc cgaatagaga gtcctcagcc gcccaggtct ttcgcgatcc
     1081 tggccagggc gatttttgtt ttcgcgagca acgccggcgc tcgatcgcgc gactggagga
     1141 aggtgcagta ggcacccaat tctcccaacc gctcgtggaa gaccgcccgg gccgcgtcct
     1201 tgtgggtcgg gatcgtcgcc ccgcgcgaca gccggaagct gattcccgcc gtcttgcctg
     1261 gatgggacgg cccggccggc gacctggtca gcgcatctgc aagcggtacg acgatgtcga
     1321 tcatcaacgt ctcggccgct cgagaaagcc gctgcagttc atcgtcggat tcttcggcgc
     1381 gcacgaagag ccggccgagg atctgcacca tcagctcgta acacgcatcg ctcagattgc
     1441 tcaattcagc ggtgtcgggg tcgtcaagga aattgagatc ggtgctgtca gcctggtcga
     1501 tcggctgcaa ggcgtaggga ttggtcagca cgggtcgagt cggctggaac tccggggcct
     1561 cggcgagcgt gtctttgtac tcctgccaga tggtcttgaa cgtgttgtag tgcgaatcag
     1621 gtgtgtcgcg gctcgcgccc tcgccctgca cgacgatcac gttgatcgcc tcgagtgccg
     1681 acgcgaggtc ggacaccggt ttcaactgcg gcaggcttcc gaagtgcagc ggagcggcaa
     1741 tctgtgcttc cggcccgctg atgaagagcc ccttctcgcc atacttctga gccagataga
     1801 cgaagccatc ctcgatgccg cgatagaggc ggccctgtgt gtgatactcg cgctcgctcg
     1861 cgaagatgtc gctcaggcgc ggttggacct cagcaggcag gctgcgttcg acctttccgt
     1921 cgtggccgac gagctcgtcc ggctcctcga tggcgatgaa ctgctcgatc gtctcgaggc
     1981 tgaagggcgc caatcgcagg tgaaagccgc cgcgacgatc catccgaatc ggaaagttcg
     2041 gccgccggag gtggggcgct ccgccgattg ccgtcaggag gttgcacacg gccccaaggt
     2101 ggagcatctc ttcgacggcc acgcctcgaa gcagccggct ccacttgcgt atcttccgaa
     2161 gctgggcatc cgtgacgcct tcgtccgtgc tgctcttcag gctgaacgcg gcgaacaggt
     2221 agcagcacat cacgccgtgc tcgatttccg ccgcctcggt caggaggtag atcaattgag
     2281 aacggttttc tatgcgtaac gttgtctcca tggttctcga ttggcttgcg gcctacggcg
     2341 cctgaaaacc caccgtttcg tgcgtcccgt cgcagaaagg cttgttcgac gattgtccgc
     2401 accgacacaa cgccgcgcgt gtctctttgc ggatcacctc cccgttcacg tcaaagaagg
     2461 tgaggttgcc gcgcacgaag agaggcccgt tcgcttgcgg ttccaccgtc gtttccgttg
     2521 gcaccggctc cggcgctccg ccgtcaaccc ggcggaagtg caacgcgcct gaaggacact
     2581 tcaagactgt ctgcacgatc cgctcggcgt ccgccgcctc gacgcgaatc cacggcctct
     2641 gcttgcggtc gaagacctcc ggcgccgtcc tgaggcagtt ctcggtgtgg atgcaatacg
     2701 cgggttccca ggtcacgacg atgtggctgc ccttgtattc cttcaggagg cctggtggcc
     2761 ttccttctcc ttgtcccatc acgcactcct cgcctgacgc ggcccgtgct ctagccaact
     2821 gagctacggg cgctcgactg caatggtact cgctggctga cgaaacgcgc gcgggcgggc
     2881 tgggcatgca gcttgctggc gcctactggc ggaggtgggt catgtccgtg agaagcattc
     2941 tggttccgac cgatttcagc gagacatctg aggcggccct ccattacgcg acggagatgg
     3001 ccctgacact gggcgcacgc ctctatctga tgcacgtgcc ggggaagacc ggcgagcact
     3061 atgaggcgaa atacccgcac ggacaattcg agacagcgac ccgcgagcgg ctctcatcct
     3121 tcctgacgaa ggaagaactc gagcgcctgc gtcccgaata cgccctgcgt gtcggtccgc
     3181 cggccgagga aatcgtgcac tacgcggaaa tctgcgacat cgacttgatc atcatgggca
     3241 cgcacggtcg cagcggtatc gcgcatgcgc tgatgggcag cgtggcggag caggtcgtgc
     3301 gagcggcgcc atgcccggtc ctgctcgtcc gcgcgcccaa gcgtgcagcg gtgacgcagg
     3361 gcgccgccgt gagagtgcca cccgtcaagc ggatgctcgc atgaaggcaa tggcgtgacg
     3421 cgaaattccc cactgtagcg aaggatttcg cacgtggtag ggctgcactc tcggacctca
     3481 ctagaatgaa ggcgagagca cgcgatcgag accgtcggtc tgaccaagta ctacggacat
     3541 ctgtccgggc tggcagggtc cctgccggcc cggctccggg cgtcggcgtc gccgccaatc
     3601 cgggcgctcg aatcgcttga catcgccgtt cgcgggggcg aggtcttcgg gttcctgggg
     3661 cccaacggct ccggaaaaac gaccacgatt cgcctgctgc tcgggtttct tcacccgact
     3721 gccgggcagg ggcgcgtgct cggactcgac atggtgcgcg acagcgtggc gatacgccgc
     3781 cggctggggt acctgccaag cggaatcgcg ctctacgaca cgctcacggg agaacagctg
     3841 ctcgattacc tggccgatct gagcggccgt ccggcctcgc gccgcgcggc attgtgtgag
     3901 cggctggagc tgtcggcgcg cacgctgaag cggctcgtcc gggactactc tcgcggcatg
     3961 cgccagaaga tcggcatcgt ccaggcgctc cagcacgacc cggagctggc gattctcgac
     4021 gagccgaccg aagggctcga cccgctcatg cagcgggcgt tctacgaaat catcgacacc
     4081 ctcaaggccg aggggcggac gatcttcttt tcgtcgcacg tgctctccga ggtggagcgc
     4141 gtctgcgatc gcgtggcaat cgtccgctcc gggcgactcg tggcgctgga ggagacggcc
     4201 aggctcgtgg cccggcggaa gcgcaacatt acgctgcgat tctccggacc gcccccgaga
     4261 ctcgaggcgg tgtccgggat cacgaacgtc gagcagggcg atggctgcct gacgtgtcgt
     4321 ctcgagggga acgtgcgtcc gttcctggct gccatcgctg acgcggcggt cgtggatctc
     4381 acgatcgagc ccgcgcgcct cgaggaagcg ttcctcgagt tctacgatga ggccccgagc
     4441 gcatgaacgc tgccctcctg cggcacacct ggcgtttgca acggaaccgg ctcgcgatcg
     4501 tctcgatcgc gctgaccgtc tggggcttcc tgccccccat catctatgcg cggttcggct
     4561 ctcagttcac ggctctcgtg cagagcgggt tgctcccgga gcagttcgcc cgcgtcggcg
     4621 gcggagacat cttcagcctc tccggtgcga tcgccctcag cttcatccat ccgatcgcca
     4681 ccattctgac ctccgtattc gcggtgggtt tttcggccgc ggcagtcgcc ggcgagcggc
     4741 agcggggcac gctcgagctc gccctggcgc ggccgatttc caggcgagtg ctctatctca
     4801 gcctgctggc agcctcgttc ggattcgtcg ccatctccat ggcggcactg gtggctggca
     4861 gcgtctccgg ggcgactttc gcgggtgtgg tcaaggaact ggcgttcgga aacctgccgc
     4921 tcctgtggct gaacagcgtc ctgctgtttg gagcctttgc ggcaatcgga ctcgccgcat
     4981 cggtctcgtt cgaccggctc gcaccggcgt tcggcacggc gctgggcatc gtggtcgcca
     5041 tgtacgtcct tgaagtgctc ggttccctct ggcccgcggc ggaattcctg cagccgtact
     5101 cgctgttcca ctacctcaag gccagagcca tcctgaacgg cctggctgcg cccttcgaca
     5161 tgttcctcct ctcgtcggtc attgtgatcg cgatggtgtg gtccctggcg gtttttcccc
     5221 gccgtgacct cgcggcgcca agctgacgtt catcgtgcga caatgccgcg catgattaca
     5281 tccattccct cgcacgctcg caccctcgca ctctcgcttg gcgcagggat ggtggcagcg
     5341 gctctcctct tcctgatcgc ctcatcaatc tcgatcgacg cgcaggctcc gcgctccgtg
     5401 caggacggcg tgttcagcga cgcgcaggcg gtccgtggcc aggcgcttta cgtgcagcgg
     5461 tgcgccggct gccacggtcc cgcgctggag ggcgcgcagg cgccgccgct gatgggcgct
     5521 gcgttcacca cgaagttccg cgccgaaccg ctgtcggcgc tgttcattca gatccgatac
     5581 gccatgcctc cgaacgcagc cgcggaggcg cagttgacgt ccgagcaggc ggccgatctg
     5641 gtagcgcaca ttctcaagac gaacggtttc cccgccggca agactgactt tgcgggagcg
     5701 gacgggccga gcagtcagat tggatggcct tctgcaggtg ccgcgggtgg caacaccgcg
     5761 gcacccgcaa cgatgtaccg tcccgccggc aacctcaatc agttgatgcg cggcgtcttc
     5821 tttccgaatt cgaatctgat cttctccatt caggaggtgg aggcagcaga cctgccgccg
     5881 ccgcccccgg cctcga
//
LOCUS       DBNGSH010000807         2642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2808484, whole genome shotgun sequence.
ACCESSION   DBNGSH010000807 DBNGSH010000000
VERSION     DBNGSH010000807.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2808484"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="AC16CY_18545"
     CDS             <1..351
                     /locus_tag="AC16CY_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355858.1"
                     /translation="GRFEIWVQPYPGPGAPVRVSPDGGVEPVWARNGRELFYLEGRTL
                     KSVAVNAGSTFDFKPATTLFEAVYTLNGQPPSYDVAADGRFLMIKPLGEPTASTMVVV
                     LNWFDELTRRVQAR"
     gene            446..1021
                     /locus_tag="AC16CY_18550"
     CDS             446..1021
                     /locus_tag="AC16CY_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR3355859.1"
                     /translation="MRVLLLSTLLSGLALQTGVAAQGSGGWTSLFDGSTLKGWNVVGN
                     ANWSVAEGAIQASMGTGFLVTPTPYRDFQITLEFWVTDDANSGVFIRCADPKTISATN
                     SYEVNIYDKRPDQSYRTGGIVDVAKPASVVMTGGKWNTFDITARGTKLTVILNGMKMV
                     DVDDAKYPNGPIALQYGAGTVKFRNVRVRTL"
     gene            complement(1062..2510)
                     /locus_tag="AC16CY_18555"
     CDS             complement(1062..2510)
                     /locus_tag="AC16CY_18555"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3355860.1"
                     /translation="MAIALMPFRRRPLVCRVTALALLFLVFDAPAAIAQRTATVQDPL
                     ERLNEAVDALTRKVWPSVVQIQVTGFGASGRTADGDVGVIGRQQSVGSGFVIDTDGYI
                     MTNAHVVNNAQRVQVVVPPTNADGTLKTALSGKMTILPARIVGIASEIDLALLKVDGV
                     RLPALPLATYSQLRQGETVYAFGSPGGLRNTLTHGMVSAVARQIDPDSPLIYIQTDAP
                     INPGNSGGPLVNVRGEVVGVNTFILSQSGGSEGLGFAVPSATARTAFRQLQKYGQLRR
                     QEVGMSLQTITPTMATALGLSRDYGVIVSDVWPRGPAEAAGMKVGDVLVSVDGQPAEN
                     LPTVSYNFRLRDSPENVQIVVLRGGTQQTLSVAAVEDRNELDSLSTMSDVERNIVPEL
                     GILGIAIDAKVVAVASGLRDPYGIIVVARVSGAKTEIPLQPRDVIRSVNNKMIITLRS
                     LQDAVRALTPGSPVTLQIQRDGKLTFVSFTYE"
BASE COUNT          460 a          870 c          854 g          458 t
ORIGIN      
        1 ggccggttcg agatctgggt ccagccgtat cccgggccgg gcgcgcccgt tcgcgtgtcg
       61 ccggacggcg gagtcgaacc ggtgtgggcg aggaacggcc gggagctgtt ctacctcgaa
      121 ggcaggacac tcaagtcggt ggcggtgaac gccggatcga cgttcgactt caagccggcg
      181 acgacgctct tcgaggcggt ctacacgctc aacggacagc ccccttctta cgacgtcgct
      241 gccgacggcc gcttcctgat gatcaagccg ctcggcgagc cgacggcgtc aacgatggtc
      301 gtggtgttga actggttcga cgaactcacg cgtcgcgtcc aggcgcgttg acagccggac
      361 ccgtcgtacg tagtgtccgg ctttagccgg accgtcggag tgtccggctt cagccggacc
      421 gcgagatgga ggttttatga cacgcatgag agttctgctc ctgagcacgc ttctgtcggg
      481 tctcgcgctg cagacaggag tcgccgcgca gggcagcggc ggctggacct cgctgttcga
      541 cggttcgacc ctcaagggtt ggaacgtcgt cggcaacgcc aattggtcag tggcggaggg
      601 ggccatccag gccagcatgg gaaccggatt cctcgtgacg cccactccgt atcgcgactt
      661 ccagatcacg ctggaattct gggtgaccga cgatgccaac agcggcgtgt tcatccggtg
      721 cgcggatccg aaaacgatca gcgctacgaa ttcctacgaa gtgaacatct acgacaagcg
      781 gccggaccaa tcctaccgca ccggcggcat cgtcgacgtc gcaaagccgg cgagcgtcgt
      841 catgacgggc gggaagtgga acacgttcga catcacggcc cgtgggacga agctcaccgt
      901 catcctcaac gggatgaaga tggtggacgt cgacgacgca aagtacccca atgggccgat
      961 tgcgcttcag tacggcgccg ggacggtgaa gttcaggaac gtccgcgtca ggactctgta
     1021 gacgtcctgg caccagtgaa ggcgcggccg gaggccgggc gtcactcgta cgtgaacgac
     1081 acgaacgtca gcttgccgtc gcgctgaatc tgcagcgtca cgggcgagcc tggcgtcagc
     1141 gcgcgcacgg cgtcttggag gctccgcagc gtgattatca tcttgttgtt cacgcttcga
     1201 atcacgtctc tgggctgaag gggaatctca gtcttggcgc ccgagacacg ggccacgacg
     1261 atgatgccgt acggatccct cagcccgctg gccacggcca cgaccttcgc atcgatcgcg
     1321 atgccgagga tgccgagctc gggcacgatg ttccgctcga cgtcggacat cgtggacagc
     1381 gaatcgagct cgtttcgatc ctcgacggcc gccacgctca aagtctgctg cgtgcctccg
     1441 cggagcacga cgatctgcac gttctccggc gagtccctga gacggaagtt gtagctcacg
     1501 gtcggcaggt tctcggccgg ctgcccgtcc accgacacga ggacgtcgcc gaccttcatg
     1561 ccggcggcct ccgcgggtcc gcgcggccag acgtccgaca cgatgacgcc atagtctcgc
     1621 gacaggccaa gcgcggtggc catcgtcggc gtgatcgtct gaaggctcat tcccacttcc
     1681 tgccgccgca gctggccgta cttctggagc tgacggaacg ccgtccgggc cgtcgcgctc
     1741 ggaacggcga aaccgagccc ctcgcttcca cccgactgcg acaggatgaa cgtgttcacg
     1801 cccaccactt cgccgcgaac gttgaccaac gggccacccg agttccccgg attgattggc
     1861 gcgtcggtct ggatgtagat gagcggcgaa tcaggatcga tctgcctggc gacggccgac
     1921 accatgccgt gcgtgagcgt gttccggagg ccgccggggc tgccaaacgc atacaccgtt
     1981 tccccttgtc gcagctgcga gtacgtcgcc aggggcaacg ccgggagcct cacgccatcc
     2041 accttgagca aagccagatc gatctcgctg gcgatcccga cgatacgcgc cgggaggatg
     2101 gtcatctttc ccgagagcgc cgtcttgagc gtgccgtccg cattggtcgg cggcacgacg
     2161 acctgcacac gctgcgcgtt gttcaccacg tgcgcgttgg tcatgatgta gccgtcggtg
     2221 tcgatgacga accctgaccc gacggattgc tgccggccga tgacgccgac gtcgccgtcc
     2281 gcggtccgcc cgctcgcccc gaagccggtc acctgaatct gcacgacgct gggccagacc
     2341 ttcctggtca gcgcgtccac ggcttcgttc agccgctcca gcgggtcctg cacggtagcg
     2401 gttcgctgcg cgattgcggc gggtgcgtcg aagacgagga agagcagggc cagagccgtt
     2461 acgcgacaga cgagcggtcg ccgtcggaac ggcatcagcg cgatcgccat tcgggtacct
     2521 ccaccgagcc aagcctaaac gatcgcgacg ttgcaaccta gtctattgaa tcagtctcgc
     2581 ccatctccga atggtccggc tgccgccttc gcgcacgacg cgcgcttcgg cgagcctcgc
     2641 cg
//
LOCUS       DBNGSH010000808         2694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1789872, whole genome shotgun sequence.
ACCESSION   DBNGSH010000808 DBNGSH010000000
VERSION     DBNGSH010000808.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1789872"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="AC16CY_18560"
     CDS             complement(<1..328)
                     /locus_tag="AC16CY_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355861.1"
                     /translation="MRMLALISALALVPATATAQNSSNSRWSTWLGCWELVTDRSDED
                     APDEQQPDAPSLKPGAAALPQVCVRPAPGGATFTTSAANQPPLEQTVIPDGANHPIVQ
                     QDCRGTQ"
     gene            complement(448..1215)
                     /locus_tag="AC16CY_18565"
     CDS             complement(448..1215)
                     /locus_tag="AC16CY_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355862.1"
                     /translation="MRYLLPALLVLTTGTGAIAGPPEEQDRPPFQAPRSSPDFLFGRP
                     DGSVGVRGSWVFARASSDWFDFVGEQLTIDDGAFNAPAIGADVAFSMSPRLDAVFSVD
                     YSSRKAFSEYRSFAENGLPINQTTELRGVNLGGSLKFSLTPRGDEVGRLAWVPRGVVP
                     YVGAGGGFYWFEMLQYGDFVDYTDYSIFTDAFESSGWTPSVSLFGGADVKLHRRVFLN
                     LEARYLWATGDLGPQWVGFEPIDLAGLRVATGINFVF"
     gene            complement(1423..2628)
                     /locus_tag="AC16CY_18570"
     CDS             complement(1423..2628)
                     /locus_tag="AC16CY_18570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3355863.1"
                     /translation="MLVALLLTTLLAGPGADAQAQVPTQAEAEELARQGQHVEALDAF
                     RRRAANNPNDLNARLWIARLHEEMGRPDLAEPVYRSVLLEAPDNVDAAIGIGRTLVSL
                     HRGEDAITALGRATQLAPKDPNIAASLGAAHLQAGNIKLGLSYLDMAASVAPTPENQL
                     ALEDARRLHGHRVEASAFYEQFSEGVPDAGNGNLLVDYRLHDRFRVMGRGQYQRKFDV
                     SDQRGGGGFQWQPPYMTVVVSALFGPGNDVLPEADALFQVGQTVRYGTWDLQYRYVSF
                     PVAHVSVFSPGIRWEGDDAWFGVRYALAITDYASFLNNEDSHTAAVESGYRVRPRVWV
                     NVGYIYGVDDFDTLSPDRLGAFKAHTIRGGVRIDLASLTSIVGGYDYQWREDDSSMSR
                     VTVKLIQSF"
BASE COUNT          478 a          856 c          891 g          469 t
ORIGIN      
        1 gctgcgtgcc ccggcaatcc tgctggacga ttggatggtt ggcgccgtcc gggatgaccg
       61 tctgctcgag gggcggctgg tttgcggcgc tggtcgtgaa cgtcgcgcca cccggggccg
      121 gccgcacgca gacctgggga agggcagccg ctccaggctt caacgagggc gcgtctggtt
      181 gttgctcgtc gggtgcgtcc tcatccgagc gatcggtcac gagctcccag catccaagcc
      241 aggtactcca gcgggagttc gacgagttct gtgcggtagc cgtggcaggg accagggcca
      301 gcgccgaaat gagagccaac atcctcatgg tgtcgctcct ctaatggtat gcccttcgcg
      361 gactactacc ggccgctccg ttgtcgcggc cggagaattg cggtcgaagc tcgttgttcg
      421 cggtactctt ccagttcata cagtccacta gaacacgaag ttgatgccgg ttgctacgcg
      481 aaggcctgcc aggtctattg gctcgaagcc gacccactgc ggtccgaggt ccccggtagc
      541 ccaaaggtac cgcgcctcga gattcaggaa gacgcgccgg tgcagcttca catcggctcc
      601 tccgaatagg gacacacttg gtgtccagcc gcttgactcg aaggcgtcgg tgaagatcga
      661 atagtcggtg tagtcaacga agtcgccgta ctggagcatc tcgaaccagt agaagccgcc
      721 tccggcgccg acgtacggca cgactccacg cggcacccat gcgaggcgcc ccacttcatc
      781 gccgcgcggt gtcagcgaga acttgaggct gccgccgagg ttcaccccgc gaagctccgt
      841 ggtctggttg atcggcaggc cgttctcggc gaagctgcgg tactcggaga acgccttccg
      901 ggagctgtag tccacgctga agaccgcgtc gagccggggt gacatggaaa aggcgacgtc
      961 ggctccgatg gcgggcgcgt tgaacgcgcc gtcgtcgatc gtcaactgct cgccgacgaa
     1021 gtcgaaccag tcgctgctgg cgcgcgcaaa cacccagctg ccgcggacgc caacggagcc
     1081 gtctggacga ccgaacagaa agtcgggtga ggaccgcggg gcctggaacg gcggtcgatc
     1141 ctgctcctca ggcgggccag cgattgcgcc cgtccccgtt gtcagaacga gaagtgccgg
     1201 cagcaagtag cgcatgccga gctggaccgc aaacttcggg ccacagcggg cgatgaaaaa
     1261 cccctgaaaa acccgggatg aaaagggccg accgtctttc gaaggcgacg gctgtggtgt
     1321 ccttcggaca gggcgacagg ggtcaacgcc caatatccaa ctcccaactc ccaaacacag
     1381 atgaattgga aattggaggt tgggcgttgg gagttgacgg gcctagaacg actgaatgag
     1441 cttgaccgtg acgcggctca tcgatgaatc gtcctcgcgc cactggtaat cgtatccgcc
     1501 cacgattgat gtcagggagg ccaggtcgat cctgacgccg ccccggatcg tgtgtgcctt
     1561 gaaagcgccg aggcggtctg gagaaagcgt gtcgaaatcg tccacgccgt agatgtatcc
     1621 gacgttgacc cagacccgtg gtctcaccct gtagccgctc tcgaccgccg cggtgtggct
     1681 gtcctcgttg ttcaggaacg acgcgtagtc ggtgatcgcg agcgcgtagc ggacaccgaa
     1741 ccaggcatcg tcgccctccc agcggatgcc gggcgagaag accgatacgt gcgcgacggg
     1801 aaagctgacg taccggtact gaaggtccca cgtgccgtag cgcactgtct gcccgacctg
     1861 aaagagcgca tcagcctcgg gcagcacgtc gttgcccggc ccgaacagcg ccgatacgac
     1921 gacggtcatg tacggcggct gccactggaa gcccccgccg ccccgctgat ccgagacatc
     1981 gaacttgcgc tggtattggc cgcgtcccat gacgcgaaaa cgatcgtgca gcctgtagtc
     2041 gaccagcagg tttccgttgc cggcatcggg cacgccctcg ctgaactgtt cgtagaacgc
     2101 cgaggcctcc acccgatggc catggagtcg gcgcgcgtct tcgagcgcga gctggttttc
     2161 cggcgttggc gcgaccgaag cggccatgtc cagataggag agcccgagct tgatgttccc
     2221 cgcctgcagg tgcgcagcgc cgagcgacgc ggcgatgttg gggtccttcg gcgcgagctg
     2281 cgtggcgcgg ccgagcgccg ttatagcgtc ttcgccccgg tgcaggctca ccagcgtgcg
     2341 gccaattccg atcgcggcat ccacgttgtc tggcgcctcg agcagcacgc tgcggtagac
     2401 cggttcggcg aggtccggcc gtcccatttc ctcgtgcagg cgggcgatcc acagccgggc
     2461 gttgaggtcg ttgggattgt tcgcggcgcg cctgcgaaac gcgtccaggg cctcgacatg
     2521 ctgtccctgt cgtgcgagct cctcggcctc ggcctgtgtg ggaacctgag cctgcgcgtc
     2581 ggcaccgggg ccggcaagca gcgtggtcag cagcaacgcg acaagcatcc agtcacctcg
     2641 ataccctcct gttttcggct gcggctgcgg caggcgttag ggacatcttg gtcc
//
LOCUS       DBNGSH010000809         4839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2468981, whole genome shotgun sequence.
ACCESSION   DBNGSH010000809 DBNGSH010000000
VERSION     DBNGSH010000809.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2468981"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..2060
                     /locus_tag="AC16CY_18575"
     CDS             66..2060
                     /locus_tag="AC16CY_18575"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3355864.1"
                     /translation="MTRYLVAALVATALPATPLEAQTADRAAVDQTIAAVYPSLVRIS
                     AVVVDYRDGREVNLEVSGSGTIITPDGFVVTNHHVAGRARRIVCTLADRQEVPADLVG
                     TDPMSDIAVLKLRPATPRSFPAARFGDSSTLRRGDPVLAMGSPRALSQSVTLGVVSNT
                     EMTMPGQSSLDLDGEDVGMIVRWIGHDAAIHPGNSGGPLVNLSGEIIGVNEISLGLGG
                     AIPGNLARSVAESLMKTGRVTRSWTGFEVQPLVGRLQGGSPGALIAWVASSSPASEAK
                     LQAGDVLVRVNETPIDARFAEQLPIVNQVLFGLPIGKPARLAVRRGSGDITVSLTPVD
                     RPPASSIPREIRQWGVVASNITPFEARGLGRTANDGVRIVSLRRGGPAEQAKPPMLRD
                     DVIVQVEGRRAGSLAELEALVRELAGGRDRASMLVTFERGHERRLTVIEVAGDRPTEA
                     PPAEAKKAWLAVETQVLTPALADRLGLKGRTGVRVTHVADSTLPLRVGDAILAIDGQP
                     VRATAPTDEEVFETTIRRFAIGATVNLTVSREGMELTMPVVLGSSPALAREMKRYEDS
                     DFGFRVRDIADSDRSDPRWDARSTSGVLVDNVSQGSWASLAQLAGGDVILALDDQPIA
                     GVDDLAARFEDVKRRRPASVVLFVRRGVKTLFIDLEPAWR"
     gene            2072..2839
                     /locus_tag="AC16CY_18580"
     CDS             2072..2839
                     /locus_tag="AC16CY_18580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HIR3355865.1"
                     /translation="MHRYTAAVVCVCACAWSAPVGAQTPEERGAARALIAKQGDAVVT
                     VLGSGKVRITQGGREVQNRDERIRAIATVLEPSGLAVMALSVLDPGDLLQGTLSRGRP
                     AGTPPIAVTTEAADLRFRLADSREIPVQVVLRDKDLDLAFLRPVDPPAKPMTEVSRTT
                     SKASAIDPVVVLTRLFEVANWQVSASFASIMAVVEKPRTYYLLNSGGFGSPVFDAQGR
                     FVGIVVRLRTDDDQGTAGAPPVVLPADDVREVARQAK"
     gene            complement(2988..3455)
                     /locus_tag="AC16CY_18585"
     CDS             complement(2988..3455)
                     /locus_tag="AC16CY_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355866.1"
                     /translation="MPFFESDAVAWLVLGAFGGAIVSAVVPWVNAELLLLSTVAIRRD
                     ATVPLVIAVALGQMVGKSVLYWLARSARVPRAQRFASAIERWRGRFQGHPYSSVLIMG
                     VSAVTGFPPFYIISIVAGALRISYVAFLIVGSLGRLAHFALFALVPHVASRWF"
     gene            complement(3463..4263)
                     /locus_tag="AC16CY_18590"
     CDS             complement(3463..4263)
                     /locus_tag="AC16CY_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355867.1"
                     /translation="MTLTVAAALGLAAGLHGAVYGAYKDSPHESFLLRRFVRELVIAL
                     VCAVGLVVLFSAAREQSLFIFYLTVFALTRIVTELWKLFIRVEPQDGYRIPTQMHRVN
                     GVVTTPWIRWALGIGFLASIQGIWALCTMLPDEWPAAVKGLLTGLAIGTGEAIAGGYK
                     DGSIEGFHWLKFFKSPTFGAIGGLVASGHVENVHQPFLFLASIGTMRMLLELLFKMVR
                     PDYAPGKFPSMVGRFTEWTERRRHFLTPYAMTWLLYMVLCSHPSWRVP"
     gene            4468..>4839
                     /locus_tag="AC16CY_18595"
     CDS             4468..>4839
                     /locus_tag="AC16CY_18595"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3355868.1"
                     /translation="MIGAFMPRPRMMLLRVSLVALLGVVAAAQTDSHPELLAAIRRGS
                     VPDVQRLLKSGASPEARDSDGTSALMAATLYSNAPLMEILLEHGADPNRTGAGGTTAL
                     MWAVPDLGKVRLLLARGANVNA"
BASE COUNT          796 a         1593 c         1683 g          767 t
ORIGIN      
        1 catgcacatc atcttaccgt gaagagtccc ttgaccaacc cgcgcctctt gcacatgatt
       61 cccgcgtgac tcgatatctg gtcgcagcgc tcgtcgcgac ggcgctcccg gcaacgccgt
      121 tggaggccca aacggccgac cgggccgccg tcgatcagac gatcgcggcg gtgtacccga
      181 gcctggttcg catctcggcc gtcgtcgtgg actatcgcga cgggcgggag gtgaatctcg
      241 aggtatcggg gagcggcacg atcatcacgc ctgacgggtt cgtcgtcacg aaccatcacg
      301 tcgccggtcg tgcacggcgc atcgtatgta ctctggctga caggcaggag gttccggccg
      361 atctcgtcgg cacggatccg atgtcggata ttgccgtcct gaagcttcgg cccgcgacgc
      421 cgcgaagctt tcccgcggcg cgcttcggcg actcctcgac gctgcggcga ggcgatccgg
      481 tgctcgccat gggaagcccg cgcgcgctgt cgcagtcggt gacgctcggg gtcgtgagca
      541 acacggagat gacgatgccg ggccagtcga gcctcgatct cgacggtgag gatgtcggca
      601 tgatcgtccg ctggatcggt catgacgcgg ccatccatcc ggggaactcg ggcggtccgc
      661 tcgtcaacct ctcgggcgag atcatcggcg tcaacgaaat cagcctcggc cttggcggcg
      721 cgattccggg caacctcgcc aggagcgtgg ccgagtcgct gatgaagacc ggccgcgtga
      781 cgcgcagctg gacgggcttc gaggtgcagc cgctcgtcgg ccggcttcag ggcggctcgc
      841 cgggcgcgtt gatcgcgtgg gtggccagct cgtcgccagc atcggaggcg aagcttcagg
      901 cgggcgacgt cctggttcgc gtcaacgaaa cgccgattga tgcgagattc gccgagcagc
      961 tcccgatcgt caaccaggtc ctgttcgggc tcccgattgg caaaccggcg cggctggccg
     1021 tgcgccgcgg gtccggcgac atcacggtct cgctgacgcc ggtcgatcgc ccgccggcgt
     1081 cgtcgattcc gcgcgagatc cgccagtggg gagtcgtcgc ctcgaacatc acgccgttcg
     1141 aggcgcgcgg gcttggacga acggcgaacg acggcgtccg tatcgtcagc ctgcgccgcg
     1201 ggggtccggc cgagcaggcc aagcctccga tgctgcgcga cgacgtgatc gtgcaggtcg
     1261 aagggcgccg cgcgggctcg cttgcggagc tcgaggcgct ggtacgcgaa ctggccgggg
     1321 gacgcgatcg cgcgagcatg ctggtcacgt tcgagcgcgg ccacgagcgc cgcctgacgg
     1381 tgatcgaggt ggccggcgac cggccaacgg aggcgccgcc cgcagaggcc aagaaggcgt
     1441 ggctggcggt cgagacccag gtattgaccc ctgcgctggc cgatcgactc ggcctgaagg
     1501 gccgcaccgg cgttcgcgtg acgcacgtcg ccgattcgac gctgccgctt cgagtaggcg
     1561 atgcgattct cgcgatcgac ggacagccgg tgcgcgcgac ggcgccgacc gacgaagaag
     1621 tcttcgaaac cacgatccgg cgatttgcga tcggcgcgac cgtcaacctg acggtgagcc
     1681 gcgagggcat ggagctgacg atgcctgtcg ttctcggatc gtcgccggcg ctggcgcgtg
     1741 aaatgaaacg ctacgaggac tctgacttcg gcttccgcgt gcgcgacatc gccgacagcg
     1801 accgcagcga tccgcgctgg gacgcgcgca gcacctccgg cgtgctcgtg gacaacgtca
     1861 gccagggcag ctgggcgtct ctggctcaac tggcgggcgg agacgtgatc ctggcgctcg
     1921 acgatcagcc gatcgccggt gttgacgatc tcgccgctcg tttcgaggac gtgaagcgcc
     1981 gccggccggc atcggtcgtg ctgttcgtgc gtcgcggcgt gaagacgctc ttcatcgacc
     2041 tcgagcctgc atggcgataa ggttggggga catgcaccga tacactgcgg ccgtcgtctg
     2101 tgtctgcgcg tgcgcgtggt ccgcgccagt tggcgcccag acgccggagg agcgcggcgc
     2161 cgcgcgcgcg ctcatcgcca aacagggtga tgccgtggtg accgttctcg ggtcgggcaa
     2221 ggtgcgcatc acgcagggcg gccgcgaggt gcagaatcgc gacgagcgga tacgcgccat
     2281 cgcgacggtg ctggagccgt cgggcctggc cgtgatggcg ctcagcgtgc tcgatccggg
     2341 cgatcttctg caaggcacac tgtcgcgcgg ccggccggcc ggtacgcctc cgatcgcggt
     2401 gacgaccgag gcggcggacc tgcgcttccg cctggccgac agccgtgaga tccccgtaca
     2461 ggtggtcttg cgcgacaagg atctcgatct cgcgttcctg cgaccggtcg atcctcccgc
     2521 caagccgatg acggaggtct cacgcacgac gagcaaggcg tcggcgatcg atcccgtcgt
     2581 ggtgctcacg cggctcttcg aggttgcgaa ctggcaggtg tctgcgtcgt tcgcgtccat
     2641 catggcggtc gtcgagaagc cgcggaccta ctatctgctc aacagcggag gcttcggctc
     2701 cccggtcttc gacgcgcagg gtcggttcgt gggcatcgtg gtccgtctgc gaaccgacga
     2761 cgatcagggg actgcggggg cgccgccggt cgtgcttccg gccgacgacg tgcgcgaggt
     2821 cgccaggcag gccaagtaat tggtccttgg tgcttggtgc ttggtccgtc cgtccctggt
     2881 ccctggtccg tccctggtcc ctggtccgtc cgtggttcct ggacatagga ccaaggacgg
     2941 accaaggccc aaggacggac gaaggcctaa ggaccaagca ccaaggacta aaaccagcgg
     3001 ctcgcgacgt gcgggacgag cgcgaagagc gcgaagtgcg ccagccggcc caggctgccg
     3061 acgatcagaa acgccacgta tgagatccgc agcgctcctg cgacgatcga aatgatgtag
     3121 aagggcggaa agccggtcac ggcgctcaca cccatgatca ggacggagga gtaggggtgc
     3181 ccctgaaagc gccctcgcca tcgctcgatg gccgaggcga accgttgcgc gcgcggcacc
     3241 ctggccgagc gcgccaacca gtacaagacg ctcttgccga ccatctgccc gagcgcgacg
     3301 gcgatgacga gcgggacggt tgcgtcacgc ctgatcgcca cggtcgacag cagcagcagc
     3361 tcggcgttca cccacgggac gacggcgctg acgatggcgc cgccgaatgc tccgaggacg
     3421 agccaggcga cggcgtccga ctcgaagaag ggcattgcgc ggttacggca ccctccacga
     3481 cgggtggctg cacagtacca tgtacagcag ccaggtcatc gcatacggcg tcagaaagtg
     3541 acggcgccgc tctgtccact ccgtgaagcg gccgaccatc gaggggaact tgcctggcgc
     3601 gtagtcgggc cgcaccatct tgaacagcaa ctcgaggagc atccgcatgg tgccgatcga
     3661 ggcgagaaac aggaacggct ggtgcacgtt ctccacgtgg cccgaggcga ccagcccgcc
     3721 gatcgccccg aatgtggggc tcttgaagaa cttgagccag tggaacccct cgatggagcc
     3781 gtccttgtag ccgccggcga tggcttcccc ggtcccgatg gccaaaccgg tgagcaggcc
     3841 cttgaccgcc gccggccact cgtccggcag catcgtgcag agcgcccaga tcccctggat
     3901 cgaggcgagg aacccaatcc cgagcgccca ccgaatccac ggggttgtga ccactccgtt
     3961 gacacggtgc atttgcgtgg ggatgcggta tccgtcctgc ggctccacgc ggatgaacag
     4021 cttccagagc tcggtcacga ttcgcgtcag ggcaaagacg gtgaggtaga agatgaacag
     4081 gctctgttcc cgggccgccg aaaacaggac gacgaggccg acggcgcaca ccagcgcaat
     4141 caccagctcg cgcacgaatc gtcgcaggag aaacgactcg tgtggcgagt ccttgtacgc
     4201 gccgtagacg gccccgtgca ggcccgccgc aagccccagc gcggcggcga ccgtcaaggt
     4261 catcgagtcg agcctcctgg cgtgtcgtgt acgtgacgtc gggacgtagg tgtcagagcg
     4321 gcgtgatcct atcgtgaggc gcgggcggca acgggcgggc cagcatactg ggtttgtacc
     4381 acgggacccg ctcgcgtctc caccgaaatc cgcccgcgcg gggggccgcc gcgcggctgc
     4441 ctgttgaaat tctacgatcc gtccagcatg ataggcgcgt tcatgccgag gccacggatg
     4501 atgctcctgc gtgtctcgtt ggttgcgctg ctcggagttg tcgcggccgc gcagaccgat
     4561 agccatcctg agttgctggc ggccatccgc cgcggatccg ttcccgacgt tcagcgcctc
     4621 ctgaaatctg gcgccagccc cgaggctcgc gacagcgacg gcacgtcggc gttgatggcg
     4681 gcgacgctgt acagcaacgc gcctctgatg gagatcctgc tcgagcatgg cgcggatccg
     4741 aatcgcaccg gcgccggcgg caccaccgcg ttgatgtggg ccgtccccga tctcgggaaa
     4801 gtccgcctgc ttctggcgcg cggcgccaac gtgaacgcc
//
LOCUS       DBNGSH010000810         5356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4168052, whole genome shotgun sequence.
ACCESSION   DBNGSH010000810 DBNGSH010000000
VERSION     DBNGSH010000810.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4168052"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="AC16CY_18600"
     CDS             <1..488
                     /locus_tag="AC16CY_18600"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR3355869.1"
                     /translation="DIEVVAAVLRRMHVECGMRDGVFEVKPSTPRAVRRIATGLWPSF
                     PSDLVSLVTVLATQAIGRTLVHDWMYELRLFALEQMSGMGADLFLADAHRIIVTGPTK
                     LRGGRTLDSRDLRSGMSLIAAGLAAEGQTRVTPLETVERGYSQLVERLKALGAKVAKV
                     E"
     gene            complement(508..2328)
                     /locus_tag="AC16CY_18605"
     CDS             complement(508..2328)
                     /locus_tag="AC16CY_18605"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355870.1"
                     /translation="MAHATSRLLASVAVSLALAGCAGSSAMRAGRAADQLQDYDRAVV
                     EYTKALRDDPENRAARQALDVAKLRAARDHFSRGRRFEATGKLDEALVELQIASELNP
                     ANGETDELLRSVRTQLRNKIATSRDGKTQLQTLVEQSRTLMPLGLELPTDVKMPASLV
                     FRDASARDVFTAIGKFANINVVFDPQFRAEPVSIDLTNSSLEDALRALTASTRTFYRV
                     TAQRTVTIVPDTATKRQEYEEEIVRPFFLSNADLKETLDLLRIVIDARRLAPVTANNA
                     IMIKDTPERVAAAGWIIAAIDKARAEVVIDVELLEVDRTRMREFGLQLASPGDPPTGV
                     NGAVTIQREGLTLRDLRNLTQSDIYLTGLPSLYYRLLKADTNTRILANPQLRTSEGES
                     AQARFGERVPVPVTTFSPIATGGLNQQPIVSYNYENIGVNIDITPRTHHDDAVSLALK
                     VTVSSVTGSGYGGLPTFANREITTVIRLKDGETNLLAGLIRDDERRVLRGVVGLSDLP
                     IIGALFAQSQREAHETDIVLTLTPRIIRVLELNEADLRPYRVGRDAGTAPLALPVPIP
                     VQPTPTEPAAAPDQPAAPPAQPRPEGATPILPPAAPPPGR"
     gene            complement(2312..2968)
                     /gene="lolA"
                     /locus_tag="AC16CY_18610"
     CDS             complement(2312..2968)
                     /gene="lolA"
                     /locus_tag="AC16CY_18610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR3355871.1"
                     /translation="MHLFAGLLVLVAAPVAAGQARQSPDALAMALQQRYQAVRDFKAD
                     FVQSYRGGVLRTATQERGTVSVKKPGLMRWIYTSPERKEFVSDGVKMYSYVPEDRQVL
                     VSSVPAGDQASTPVLFLTGKGDFVRDFTAAIVKDAAVPGTVALKLTPRRNEPDYEYLI
                     LALDPGTLQIRALTTRDHQGGESTLKFENLQENKGISDKDFAFRIPRGVDIITDGPRN
                     "
     gene            complement(2975..4225)
                     /locus_tag="AC16CY_18615"
     CDS             complement(2975..4225)
                     /locus_tag="AC16CY_18615"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR3355872.1"
                     /translation="MLASMVGREAQRAELRVSDLVPVGGLRRCAPAMHGASLLAVAAR
                     ERHSEVLSGFSRLPIVKSVIDTSDTTMRAATDRGPLELHVATPEAAGAALVWHTGSKR
                     HTAKLGRRAAARQMTFNGPLLLGRNGVEMTVRDEDTCYRLLGLPYIPPELREGEDEID
                     AADRGALPRLVSTLHIRGDLHLHSTWSDGRDSILHMVLSAQRLGYEYVAITDHSERSW
                     ASRKLLGLEIPLQRQEIESIRPQAPGIEILHGVEVDIMPDGSLDFDDEQLAGFDIVLA
                     SLHDDHGHDSQQLTERYLSAIRHPLVNIITHPANRSPALSDGYDLDFDRLFQAAVETG
                     TAMEVDGSPGHLDMDGRIARRAAAAGVTIVINSDGHRADALGRQMRFGLGTARRGWIE
                     PDRVLNARGADAVRAFVARKRAGG"
     gene            4377..4778
                     /locus_tag="AC16CY_18620"
     CDS             4377..4778
                     /locus_tag="AC16CY_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355873.1"
                     /translation="MVIALREGTFIGRPPGGVTGATLSKATPHAEQVATITVSRMSPD
                     DAGIREIFVSVDGNEIAVLRNGEVVTHEIPAGAHRLKAHNTLFWKTRDLVLAPGEHAR
                     FSAVNRTGWATFGLLIVLGAAPLYLTLEREG"
     gene            4887..4962
                     /locus_tag="AC16CY_18625"
     tRNA            4887..4962
                     /locus_tag="AC16CY_18625"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4920..4922,aa:Ala,seq:cgc)
     gene            5026..>5356
                     /locus_tag="AC16CY_18630"
     CDS             5026..>5356
                     /locus_tag="AC16CY_18630"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3355874.1"
                     /translation="MTQIKRIGVLTGGGDAPGLNAVIRAVVKTASNAGIEVIGLEDSF
                     DGLVYPEKSRPLVPRDVTGILRLGGTILGTVNRGNPFAQPIVTSEGTFDYSDRVVEMF
                     GRMKLDGL"
BASE COUNT          820 a         1711 c         1863 g          962 t
ORIGIN      
        1 aagacatcga agtcgtcgcc gccgtgttgc ggcgcatgca cgtcgagtgc ggcatgcggg
       61 acggggtctt cgaggtcaag ccgtctacgc cccgtgcggt gcggaggatc gcgacggggc
      121 tgtggccctc gtttccgagc gacctggtga gcctggtcac cgtgcttgcc acgcaggcga
      181 tcggccgcac gctggtgcac gactggatgt acgagctccg gctcttcgcg ctcgagcaga
      241 tgagcggtat gggtgcggat ctcttcctcg ccgacgccca tcgaattatc gtgaccggtc
      301 cgaccaagct ccggggaggg cgtacgctcg acagccgcga tctgcgatcg ggaatgtcgt
      361 tgattgccgc cggcctcgct gccgaaggcc agacgcgggt gacgccgctc gagaccgtcg
      421 agcgcggcta cagccagctc gtcgagcgcc tcaaggcgct tggcgcgaag gtggcgaaag
      481 ttgaataagc gcgcgcgcag gcgctgatta acgccccggc ggcggggcag cgggcggaag
      541 gatcggcgtg gccccctctg gtcgcggctg cgccggcggc gcggcaggct gatcgggagc
      601 tgccgccggt tcggtcggcg tcggctgcac tggaatggga accggcagcg cgagcggcgc
      661 cgtaccggcg tcgcgaccca ctcgatacgg acgcaggtcg gcctcgttga gttcgagcac
      721 cctgatgatt ctcggtgtca gcgtgaggac gatgtccgtt tcgtgggctt cgcgctggga
      781 ctgggcgaag agcgctccga tgatcggcag gtcgctcagt ccgaccacgc cccgcagcac
      841 gcggcgctcg tcgtcgcgga tgaggccggc cagcagattc gtttcgccgt ccttcagccg
      901 gatcaccgtg gtgatctcgc ggttggcgaa agtgggcagc ccgccgtagc ccgagcccgt
      961 gacgctgctc accgtcactt tcagcgccag cgagaccgcg tcgtcgtggt gggtccgcgg
     1021 cgtgatgtcg atgttcacgc cgatgttctc gtagttgtag gacacgatcg gctgctggtt
     1081 gaggcccccg gtggcgattg gcgagaacgt cgtcaccggg acgggtaccc gttcgccgaa
     1141 tcgggcctgg gccgactcgc cctccgacgt tcgaagctgc gggttggcga ggatcctcgt
     1201 gttcgtgtcc gccttgagca gccggtagta caggctgggc aggcccgtca ggtagatgtc
     1261 cgactgggtg aggttgcgca ggtcgcgcag cgtgagacct tccctttgga tggtgacggc
     1321 gccgttgacg ccggtcggcg ggtcgccagg ggaggcgagc tgcagtccga actcgcgcat
     1381 ccgcgtccga tcgacttcga gcagttccac gtcgatcacg acctccgcgc gggccttgtc
     1441 gatggcggcg atgatccaac ccgcggcggc gacgcgctcg ggggtgtcct tgatcatgat
     1501 cgcgttgttc gctgtcaccg gcgccagacg cctcgcgtcg atgacgatgc gaagcaggtc
     1561 gagcgtttcc ttcaggtccg cgttgctgag gaagaacggc cggacgatct cttcctcgta
     1621 ctcctgccgc ttggtcgcgg tgtcggggac gatcgtgacg gtccgttgcg cggtcacgcg
     1681 gtagaacgtc cgcgtcgacg cggtgagcgc cctgagtgcg tcctcgagcg acgagttcgt
     1741 caggtcgatg ctgaccggct cggcccggaa ctgcggatcg aagacgacgt tgatgttggc
     1801 gaacttcccg atcgccgtga agacgtctcg cgcgctggcg tcgcggaaca cgagggaagc
     1861 gggcatcttg acgtcggtcg gcagctccag gccgagcggc atcagcgtgc gcgactgttc
     1921 aaccagcgtt tgcagctggg tcttgccgtc tctcgacgtc gcgatcttgt tccgaagctg
     1981 cgttcgcaca cttcgcagca gctcgtcagt ctctccgttg gctgggttga gctcggaggc
     2041 aatctgcaat tcgacgagcg cctcgtccag cttgcccgtc gcctcgaacc ggcgtccgcg
     2101 ggagaagtgg tctcgcgcgg cccggagctt ggccacgtcg agcgcctggc gcgcggcgcg
     2161 attctcgggg tcgtcgcgca gggccttggt gtattcgacg actgcccggt cgtaatcctg
     2221 gagctggtcg gctgcgcggc cggccctcat ggcggaactc ccggcgcagc cggcgagggc
     2281 cagcgacacc gcgacggacg cgagcaaacg gctagttgcg tgggccatca gtaatgatgt
     2341 ctacgccgcg tggaatacgg aacgcaaagt ccttatcgga tatgcccttg ttttcctgca
     2401 ggttctcgaa ttttagcgtc gattcgccgc cctggtggtc ccgggtggtc aaggcgcgga
     2461 tctgaagcgt gcccgggtcc agggccagaa tcaggtattc gtagtccggc tcgttgcggc
     2521 ggggggtcag cttgagcgcg accgtccctg gaacagccgc gtctttgacg atggccgccg
     2581 taaagtccct cacgaagtcc cctttgcctg tcaggaagag gaccggcgtc gaggcctgat
     2641 cgcccgcggg cacgctcgac accagaacct gccggtcctc gggaacatac gagtacatct
     2701 tcacgccgtc ggacacgaat tccttccgct cgggactcgt atagatccac cgcatcagcc
     2761 ccggtttctt gaccgacacc gttccccgct cctgcgtcgc ggtccgcagc acgcctcccc
     2821 ggtaggactg gacgaagtcg gctttgaagt ccctgacggc ctgatatcgc tgttgaagcg
     2881 ccatggccag cgcatcgggg ctctgccgtg cctgcccggc agccaccggg gcggcgacga
     2941 ggacgaggag gcccgcgaaa aggtgcatgc cctattatcc ccccgcccgc ttacgcgcga
     3001 cgaatgcccg gacggcgtcc gcaccccggg cgttcaggac gcggtcgggc tcgatccagc
     3061 cgcgccgcgc cgtgcccagc ccgaagcgca tctgccgccc gagtgcatcg gctcgatggc
     3121 cgtcactgtt gatgacgatg gtgaccccgg ctgccgccgc ccgcctggcg atccggccgt
     3181 ccatgtcgag atggcccggc gaaccgtcca cctccatcgc cgtgcctgtt tcgaccgccg
     3241 cctgaaacag gcgatcgaag tcgaggtcgt agccgtccga cagcgctggc gacctgttcg
     3301 cggggtgcgt gatgatgttg acgagcgggt ggcgaatggc cgacaggtat cgctcggtca
     3361 attgctggct gtcgtgcccg tgatcgtcgt gcagcgaggc gagcacgatg tcgaacccgg
     3421 cgagctgttc gtcgtcgaaa tcgagcgacc cgtcgggcat gatgtccact tcgacgccgt
     3481 gaaggatttc gattcccggc gcctgcgggc ggatggactc gatctcctgg cgttggagcg
     3541 ggatctccag tcccagcagc ttccgtgatg cccacgatcg ctcggaatga tcggtgatgg
     3601 cgacgtactc gtagccgagc cgctgcgcgc tcagcaccat gtgcaggatc gagtcgcgtc
     3661 cgtcgctcca ggtgctgtgc agatggaggt cgccgcggat gtggagtgtc gacacgagcc
     3721 gcggcagcgc accgcgatcg gcggcgtcga tttcgtcctc accttcacgg agctccggcg
     3781 gaatgtaggg aagtccgaga aggcgatagc acgtgtcctc gtcccggacc gtcatctcga
     3841 cgccgttccg gccgaggagg agaggaccgt tgaacgtcat ctggcgggcc gcggcgcgcc
     3901 gcccgagctt ggccgtgtgc cgcttgctcc cggtgtgcca gacgagtgcc gcccccgccg
     3961 cctccggcgt ggccacgtga agctcgagcg gtccccgatc cgtggcggcg cgcatcgtgg
     4021 tgtcggaggt gtcgatgacc gatttgacga tggggaggcg ggaaaagccg gacaacacct
     4081 cggaatgccg ctctcgggct gccactgcca gcagtgacgc tccgtgcatg gccggcgcgc
     4141 atcggcggag cccgcccacc gggacgaggt cgctgacgcg caactcggcc cgctgtgcct
     4201 cacggccgac catcgaggcc agcatgtcag cccgtccgag ggaaatcctg ggatgtcgca
     4261 ctgtcgctcg tcgctctact ggaagtcggc cgttggtctc gaagacgtga atcggcctcc
     4321 gagcatattc cgtttccgcg ctgcaacggg tgcggggttg ggggataaaa ttgtccgtgg
     4381 tcattgcgct gcgggaaggc actttcatcg ggcgcccgcc cggtggagtc accggagcga
     4441 ccctgtcgaa ggcgacgccg cacgccgagc aggtcgcgac gatcacggtg agccggatgt
     4501 cgccagacga tgccggcatc cgcgagattt ttgtgtcggt ggacggcaac gagatcgccg
     4561 tattgcgaaa cggggaagtc gttacgcacg agattccggc gggcgcccat cggctcaagg
     4621 cgcacaacac gctgttctgg aagactcgcg atctcgtcct cgctcccggc gagcatgcgc
     4681 gcttcagcgc agtcaacaga accgggtggg ccaccttcgg gctcctcatc gtgctcggcg
     4741 cagcgccgct ctacctcacg ctggagaggg aggggtgaca agggtgcgag ggtgcgaagg
     4801 tgcgggggtg cgagggagat ggtacactga gggttcggac gcgccgtgga gccttgctct
     4861 ggtgctctcc gagcggtgaa tgcagtgggc ggctagctca gttgggagag cgccgcgttc
     4921 gcaatgcgga ggccgcgggt tcgagccccg cgccgtccac cagattcttc cgctccaacg
     4981 cctccgaggc gtaagccctc aggggcgttt cccgttcggc gcttcatgac gcagatcaag
     5041 cgaatcggcg tgctcaccgg cggaggtgat gctccgggtc tcaacgcagt catccgcgcc
     5101 gtcgtaaaga ccgcgtccaa cgccggcatc gaggtcatcg ggctggaaga cagcttcgac
     5161 ggtctcgtct atcccgagaa gtcccgtccg ctggttccac gcgacgtcac gggcatcctg
     5221 cggctcggag gcaccatcct cggcaccgtc aaccgcggca atccgttcgc ccagcccatc
     5281 gtcacgagcg aggggacgtt cgactactcc gacagggtcg tcgagatgtt tggacgcatg
     5341 aagctggatg gactgg
//
LOCUS       DBNGSH010000811         2504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_772209, whole genome shotgun sequence.
ACCESSION   DBNGSH010000811 DBNGSH010000000
VERSION     DBNGSH010000811.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_772209"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="AC16CY_18635"
     CDS             <1..1281
                     /locus_tag="AC16CY_18635"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3355875.1"
                     /translation="DPNTELHSFPRLRIVAPAAPGGGWDQTARVMQQVLERSGLVRIA
                     PVENISGAAGMIGLARFIGAERGSRDTLMVSGLIMLGGIVTHRSPVTLHDVVPIARLT
                     GEYEVIVVPAGSPFRSLADLVAALRERPEAISWGGGSAGGSDQILAGLVAEAVGVAAS
                     RINYIAFSGGGESLAAILGGQVSVGVNGLAELAAHVEAGTLRVLAVSSAERLTGLDAP
                     TLREQGVDVEFENWRSVVAPPGISREDRVRLEALVGDMVRSTEWRDALSRYRWIDRYL
                     SGSDFDRFTAAEEARVRTILRRLGTGDEESGTLASAGPYPLVILVGLVATAIVAGLQT
                     RRSSASVAPQRRSTWKPVALIAAGVTLTVLLAERAGFIVASALLFWCTARAFDARHPV
                     RDALFAIAISLAAYVLFARVLQLPLPSGLPGGWI"
     gene            1285..>2504
                     /locus_tag="AC16CY_18640"
     CDS             1285..>2504
                     /locus_tag="AC16CY_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3355876.1"
                     /translation="MLDTLRSLADGFSSALTVAHLAWALAGVTLGTAVGVLPGIGPAM
                     TVALLLPVTFTLDPTSAFIMFGGIYYGAMYGGSTTSILLNTPGETSAIATAIDGHAMA
                     RQGRGAAALTTAAIGSFVAGTLATIALTFFAPWLAGVALRFGPAEYFALTVVAFATTS
                     VLLGGSVVRGLFSLFLGLALGLVGIDPLTGNARFSFGIPQLLDGIDVVILVIGLFAVG
                     ETVYQAWRHQGDDVPPVPLRRTTGMTADEWKRSWKPWLRGTAIGFPLGVLPCGGAVIP
                     TFLSYTVEKRLSDRPEEFGQGAIEGVAGPEAANNAAAAGVLVPLLTLGLPSSATAAVL
                     LAAFAQFGLQPGPLLFTTRPDLVWTLIASLYIGNLALLVLNLPLAGVWARVMLVPRPL
                     LFAGILVLASVGAY"
BASE COUNT          314 a          879 c          875 g          436 t
ORIGIN      
        1 gaccccaaca cagaattgca ctcgttcccg cgacttcgca tcgtcgcgcc ggccgcaccg
       61 ggcgggggat gggatcaaac tgcgcgggtc atgcagcagg tgctcgagcg cagcgggctc
      121 gtgcgcattg caccagtgga gaacatctcg ggcgcggccg gcatgatcgg gctggcgcgc
      181 ttcatcggcg ccgagcgcgg cagccgcgac acgctgatgg tctccggcct catcatgctc
      241 ggcggcatcg tcacgcatcg atcgccggtc acgcttcacg acgtcgttcc gatcgcgcgt
      301 ctcacgggcg agtacgaggt gattgtggtg cctgcgggat cgccgtttcg atcgctggcc
      361 gatctcgtgg ctgcgctgcg cgagcgcccg gaagccatct cgtggggcgg cgggtcggcc
      421 ggagggagcg atcagatact ggccggcctc gtcgccgagg ccgtcggtgt ggccgcgagc
      481 cgcatcaact acatcgcgtt ctccggcggc ggcgaatcgc tggccgccat tctcggaggt
      541 caggtctcgg taggcgtgaa cggtctggcc gagctcgcag cccacgtcga agcgggcacg
      601 ctccgcgtcc tcgccgtgtc gagcgccgag cggctgaccg ggctggacgc gccgacgctt
      661 cgcgaacagg gcgttgacgt ggagttcgag aactggcgct cggtcgtggc gccccctggc
      721 atcagcaggg aggatcgggt ccggctggag gcgctcgtcg gcgacatggt gcgatccacc
      781 gagtggcgcg acgcgctgag ccgctaccgc tggatcgatc ggtacctgtc cggatcggac
      841 ttcgaccgtt tcacggccgc cgaagaagcg cgcgtccgga cgatcctgcg ccggctcggc
      901 acgggtgatg aggaaagcgg gacgctcgcc tccgccggcc cctatccact ggtgattctc
      961 gtcggcctcg tcgccacggc gatcgtggcg ggtcttcaga cgaggcgatc gtcggcttcg
     1021 gtggcgccgc aacgtcgatc cacgtggaag ccggttgcgt tgatcgctgc cggcgtcacg
     1081 ttgaccgtcc tgctcgcgga gcgcgcggga ttcatcgtcg catcggcgct gctcttctgg
     1141 tgcacggcac gcgcgttcga cgcgcggcat cccgttcgag acgccctgtt cgccatcgcg
     1201 atctcgctcg ccgcctacgt gctcttcgcg cgtgtgctgc agctgcccct gccatccggc
     1261 ctgccgggcg gctggatctg acgcttgctc gacaccctgc gctccctggc agacgggttc
     1321 agcagcgcgc tgaccgttgc gcaccttgcg tgggcgctcg ccggcgtcac gctcggaacg
     1381 gccgtcggcg tgctgccggg catcgggccc gcgatgaccg tggcgctgct cctgccggtg
     1441 acgttcacgc tcgacccgac gagcgcgttc atcatgttcg gcggcatcta ctacggcgcg
     1501 atgtacggcg gctcgaccac gagcatcctg ctgaatacgc ccggcgagac gagcgcgatc
     1561 gcgacggcca tcgacggcca cgccatggcg cgccagggcc gcggcgcggc ggccctgacg
     1621 acggccgcga tcggatcgtt cgtggcgggc acgctggcca ccatcgcgct gaccttcttc
     1681 gccccctggc tcgccggcgt ggcgttgcga ttcggacccg ccgagtactt cgcgctgacc
     1741 gtcgtcgcgt tcgcgacgac gtcggtgctg ctcggcggat cggtcgtgcg cggcctcttc
     1801 agcctgttcc tgggcctggc gctcggcctg gtcggcatcg atccgctcac cggcaacgcg
     1861 agattctcgt tcggcatccc gcagctgctc gacgggatcg atgtcgtgat tctcgtgatc
     1921 ggtctcttcg ccgtcggcga aacggtgtac caggcgtggc ggcaccaggg cgacgatgtc
     1981 cctcccgtgc cgctgcgccg gaccacgggg atgacggccg acgagtggaa acgatcgtgg
     2041 aagccctggc tgcgcggcac cgcgatcggg tttccactcg gcgtgctgcc gtgcggcggc
     2101 gcggtgatcc cgacatttct ctcctacacc gtcgagaagc ggctgagcga ccggcccgag
     2161 gaattcggac agggcgcgat cgaaggcgtc gcgggacccg aagcggccaa caacgccgcg
     2221 gccgccggcg tgctagtccc gctgctcacg ctcgggcttc cctcgtccgc gacagcggca
     2281 gtgctgctcg ccgcgttcgc gcagttcggc ctgcagcccg gcccgctgct cttcaccacg
     2341 aggcccgacc tggtgtggac gctgattgcg agcctctaca tcggcaacct cgcgctgctc
     2401 gtcctcaacc tgccgctcgc cggcgtctgg gcgcgcgtga tgctggtgcc gcgcccgctg
     2461 ctctttgcgg gcatcctcgt gctcgcctcc gtcggcgcgt acag
//
LOCUS       DBNGSH010000812         3428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3319426, whole genome shotgun sequence.
ACCESSION   DBNGSH010000812 DBNGSH010000000
VERSION     DBNGSH010000812.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3319426"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="AC16CY_18645"
     CDS             <1..203
                     /locus_tag="AC16CY_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355877.1"
                     /translation="VVYTPHAVLYHYEGSTRGLLHPQPDQQAFGEKWKDVLDRGDPYY
                     NPNLTDTHDDWSIRVEDDDKET"
     gene            404..958
                     /locus_tag="AC16CY_18650"
     CDS             404..958
                     /locus_tag="AC16CY_18650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3355878.1"
                     /translation="MSQAVRRFGDLRAWQACREYKQAVYAVCDEGPLSRDWDRRRQLE
                     ESVAGPPGHLAEGFGRFNPAEFARYTVIARSSLMESQNHLRDAVDKRYISEAKRQELD
                     ALAETALKEVTGLMEYLQSPEALRNARRARERRIASRHARRNAVESTEAASGRSNPEP
                     RTPNENREHEPEIEHEPRSEKREV"
     gene            complement(965..1501)
                     /locus_tag="AC16CY_18655"
     CDS             complement(965..1501)
                     /locus_tag="AC16CY_18655"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3355879.1"
                     /translation="MARILTGICLLVIAAGASGAAQSTRSAAGDSVSARLQRFEDKEE
                     IQNLLLDYGRHLDSRDFAAYSRLFARDGEWVGGFGSVSGPANIQAFMEKNMGTAPNKA
                     KNYHLLSNFVITVTGDTATAWSRWAFVVPGERGAAISQAGRYDDTLVREDGRWRFKRR
                     VASNDTAPPATATGRGDK"
     gene            1623..2678
                     /locus_tag="AC16CY_18660"
     CDS             1623..2678
                     /locus_tag="AC16CY_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355880.1"
                     /translation="MSLLRAAAGLSSVVAALAVLQGSALVGQGDDPFAFLKPVVTVSP
                     GDLQRLDRGEVIVRVLPAHDNQLAVFAVSRLDADPDSLIHWAGAIEQMKKSPYVLAIR
                     RFSDPPLLSDLDGLMLDDEDVEDIPRCQRGDCGVKLSEDEIVALKAAADSGGAWKAAV
                     QREFRRVLFNRVTRYRHAGLAGLPPYVDRPETSLPRNAFGALLEHSPHLRRHLPAMTD
                     ALARYPKTTLPSVDSFLYWSKEHYGRGKPVTTITQVHVVRPGPPPLPAVLMLAQEIFA
                     SHYRTGSLSTTAFVVGSADARYLVHLNRSQLDMLKGMFGGVVRSVLEGRLANEAKPAM
                     VRVKGRLESGHPSAASP"
     gene            2730..3185
                     /gene="trxA"
                     /locus_tag="AC16CY_18665"
     CDS             2730..3185
                     /gene="trxA"
                     /locus_tag="AC16CY_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3355881.1"
                     /translation="MSVLVSDPQGVLATCPSCGARNRQRYANLNRRIRCGRCKADLPP
                     PSEPVEAPDAAAFDALIAAAPVPVLVDFWAPWCGPCRMMAPELEKAARRMAGAALVVK
                     VNTDAVPELGERFGIRSIPTLAVFRHGREASRASGARSAADIETFVERA"
     gene            3185..>3428
                     /locus_tag="AC16CY_18670"
     CDS             3185..>3428
                     /locus_tag="AC16CY_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HIR3355882.1"
                     /translation="MSKVRPPTDKVLLEGPHSRRRELWLVLRALKDFITGFRTLHFVG
                     PCVTVFGSARYGESHPHYALGRHVGASLARMGFTVMT"
BASE COUNT          571 a         1129 c         1110 g          618 t
ORIGIN      
        1 tcgtcgtata cacgccgcat gcggtcttgt atcactacga gggatcgaca cgcggcctgc
       61 tgcatccgca gccggaccag caggcgttcg gcgagaagtg gaaggacgtt ctcgatcggg
      121 gcgatcccta ctacaacccc aacctcaccg acacgcacga cgactggagc atccgagtcg
      181 aggacgacga caaggagaca tgatcgagcc gttcacactt ctcacttctc ggttctcggt
      241 tcgtgttcag tgtcaggttc gtgttcgggg ttcgaggttc ggggttcgcg gattctaggt
      301 tcgttaccgc tgtttaggta tcgcaggaac tgcgtactcc gacgcgcctc tcttgtaaac
      361 cggcctgttt cgtggcggca cgtgacgtgc tctcacagca tgcgtgtcgc aggccgtccg
      421 gcgcttcggc gatcttcgtg cgtggcaagc gtgccgcgag tacaaacagg ccgtctacgc
      481 agtctgcgac gaaggcccgc tatctcgcga ctgggaccga cgaaggcagt tggaagaatc
      541 ggtcgccggt ccgccgggac acctggcgga gggctttggc cggttcaacc ccgccgagtt
      601 cgctcgctac acggtgatcg cccggtcgtc gttgatggag tcgcagaacc atcttcgtga
      661 cgccgtcgac aagaggtaca tcagtgaagc gaagaggcag gagttggatg cgctggcgga
      721 gaccgcattg aaggaagtca caggcctgat ggaatacctc cagtcgcccg aggcgctgcg
      781 gaacgcgaga cgggccaggg agcggaggat tgcgtcgagg cacgcgcgcc gaaatgcggt
      841 cgagtcgact gaagcggcgt caggccgttc gaaccccgaa ccgcgaaccc cgaacgagaa
      901 cagggaacac gaacctgaaa ttgaacacga accgagaagt gagaagcgag aagtgtgaac
      961 tgggttactt gtctcctcgt cccgtcgccg tcgcgggagg tgccgtgtcg ttcgatgcga
     1021 cccgccgctt gaagcgccag cgcccgtcct cgcgcacgag cgtgtcgtcg tagcggccgg
     1081 cctgcgagat cgccgcaccg cgctcgccgg gaaccacgaa ggcccatcgc gaccacgccg
     1141 tcgccgtgtc gccggtcacg gtaatcacga agttcgagag caggtggtag ttctttgcct
     1201 tgttgggcgc ggtgcccatg ttcttttcca tgaacgcctg gatgttcgcc ggccccgaga
     1261 ccgacccgaa gccgccgacc cactctccgt ccctggcgaa cagacgcgag tacgcggcga
     1321 agtcacgact gtcgaggtgg cgcccatagt cgagtaagag gttctgaatt tcttccttgt
     1381 cctcgaacct ctgcaggcgc gcggataccg aatcgcccgc ggctgagcgc gtggattgag
     1441 ccgcgccaga ggcaccggcg gcgatcacca acagacagat tcccgtgagt attcgcgcca
     1501 tcctgcacct cgtttcacgc ctcggcacgg tccgtaccac caaggcaggg ccagagccta
     1561 aggccgctca ccgacgagcg tcaagccggc agagggtttg tatagggcgg gagggaggat
     1621 ctgtgtcact ccttcgcgcc gccgcgggcc tgtcctcggt tgtggccgca ctggccgtcc
     1681 tccagggatc cgcgctggtt ggccaggggg acgatccctt cgcgttcctc aaaccggtcg
     1741 tcacggtcag tcccggcgat ctgcagcgtc tcgatcgcgg cgaagtgatc gtgcgggtcc
     1801 tgccggcgca cgacaaccaa cttgccgtgt ttgccgtatc gaggctcgac gccgatcccg
     1861 attccctgat ccactgggcc ggcgcgatcg agcagatgaa gaagagcccg tacgtcctcg
     1921 cgatccgccg gttttccgat cctccgctgc tgtcggatct cgacggcctg atgctcgatg
     1981 acgaggatgt cgaagacatt ccgcgctgcc agcgcggcga ctgcggtgtg aagctgtccg
     2041 aagacgaaat cgtggcgctg aaggcggctg ctgattcggg gggagcctgg aaggccgccg
     2101 tccagcggga gttccgtcgg gtcctgttca accgcgtcac tcgctatcgg cacgcggggt
     2161 tggccgggct gccgccgtac gtcgaccgtc cggagacctc gcttccgagg aacgcgttcg
     2221 gtgcgttgct cgagcactct ccgcacctcc gccgtcacct cccggccatg accgacgcgc
     2281 tcgcccgcta cccgaagacc acgcttccgt cggtcgattc gtttctctac tggtccaagg
     2341 agcattacgg acgcggcaag cccgtgacga cgatcaccca ggtgcatgtc gtgcggccgg
     2401 gtcctcctcc cttgcccgcg gtgctgatgc tcgcgcagga aatcttcgcg tcgcattacc
     2461 gcacggggtc tctcagcacg acggccttcg ttgttggaag cgcggacgcc cggtacctcg
     2521 ttcacctgaa ccgctcgcag ctcgacatgc tgaaggggat gttcggcggt gtggtgcgct
     2581 cggtgctcga ggggcggctc gcgaacgagg caaagccggc aatggtcagg gtgaaggggc
     2641 gcctcgagag cgggcatccg tcggcggcca gcccgtagcg gtctccggtt ttccatccca
     2701 ccggtgcgac aattacagta gaacgcacca tgtccgtctt ggtctccgac cctcagggcg
     2761 tactcgccac ctgcccctcg tgcggggcaa gaaaccgcca gcgttacgcc aacctgaatc
     2821 gccgtattcg ctgcggccgc tgcaaagcgg atcttccgcc gccgtccgaa cctgtcgagg
     2881 cgccggacgc cgcggccttc gacgcgctga tcgccgcggc tcccgttccc gtgctcgtgg
     2941 acttctgggc gccatggtgc ggtccctgcc gaatgatggc cccggaactc gagaaggcgg
     3001 cgcggcggat ggccggcgcc gcgctcgtcg tcaaggtcaa caccgacgcc gtgcccgagc
     3061 tcggcgagcg cttcggcatc cggtccatcc ccacgctggc ggtctttcga cacggccgag
     3121 aggcgtcccg cgcgtcgggt gcacgctcgg ccgccgatat cgagaccttc gtggaacgcg
     3181 cgtgatgtcc aaggttcgtc cacccaccga caaagtcctg ctcgagggac cgcattcccg
     3241 ccgccgggag ctctggctcg tcctgagagc gctcaaggac ttcatcaccg gattccgcac
     3301 gctgcacttc gtcgggccct gcgtcaccgt gttcggctcg gcgcggtacg gagagtcgca
     3361 tccgcattac gccctcggcc ggcacgtagg cgccagcctc gcccgcatgg ggttcaccgt
     3421 gatgacgg
//
LOCUS       DBNGSH010000813         6672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1960976, whole genome shotgun sequence.
ACCESSION   DBNGSH010000813 DBNGSH010000000
VERSION     DBNGSH010000813.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1960976"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="AC16CY_18675"
     CDS             <1..447
                     /locus_tag="AC16CY_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355883.1"
                     /translation="RTHEFRAGDVSCSDAHSHLPENVGRDTEFVQVHLKNRKTFDHLQ
                     TGRSLVYPARVKAPDPIDADPAHYFVQFENDVVRLARIKVPGGVKTPMHAHLAYCIVE
                     IRDMETGVKAGDSSCGNAMAHESLNNAKSVNEAITIEFKNRDSFKP"
     gene            complement(450..1034)
                     /locus_tag="AC16CY_18680"
     CDS             complement(450..1034)
                     /locus_tag="AC16CY_18680"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3355884.1"
                     /translation="MIRIPTSLAVVLLCAGLSGCAAASQDTARAAGALEAQDYIDIRQ
                     LIDGYSHVLDNCTNNGNTYADLFTPDATFGVSSQWGSGAKIWFRGREQLRVAGGGGKD
                     SCRPPQRYPAYHIVISPVITPAPGGAKATSTLLTITNKTTKQGDIVHWEGGYEDTFVK
                     TANGWRFKSRVHVWPEIAWTDNPADMPPRDLEKE"
     gene            1165..2517
                     /locus_tag="AC16CY_18685"
     CDS             1165..2517
                     /locus_tag="AC16CY_18685"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3355885.1"
                     /translation="MADIFISYAREDEERIRPLAAALEAHGWSVFWDRRIPAGQTWRS
                     VLGQSLHDARCVIVAWSRHSVASRWVGEEADAGLQRGILVPVLLEQVEPPIGFRSLQA
                     AELGSGPLDDGSPAFTQFVQDVKKVLGGPSSPHTVPPMRSVRTLPSSSRAAIAGLAVV
                     LLALAGAAYFYSTRVPEPANGESATNSANSSAAGSASTGSGIAPTPPAVTPSSSSADS
                     RAGEFAIKIGDKVSEDVPGPGAGIIARPFEHDVYRFTAAPRQMVYFRVREISATLQNS
                     YLGWRLADEDGTDVFKQGLYTGNPGVHTLFRGGAYTLTVGSRDDAAVGLYEFQLYDVP
                     RPDQFAIALGTRIRDGQPGPGAGSIETPGAKDVYTFKAAPRQQVSARVLGGSPAILNS
                     YLGWLLVDEDGTEIFKRGLYTGHPGVLTLIRGGTYTLTVGSDVDPATGTYDVQIDAQN
                     "
     gene            complement(2576..3364)
                     /locus_tag="AC16CY_18690"
     CDS             complement(2576..3364)
                     /locus_tag="AC16CY_18690"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3355886.1"
                     /translation="MDLTQSLLVERHLAESFLRERVVPGVEVHRDRDVVWIVHGGQAW
                     RNAGIMVRFPAGGAGQRLDTLVRRYERHRRGMALWISPSATPDNLPALLHDRGLRCRK
                     YYPAMVRHLAEHVPVRSRAAALEIRPVTDAAEYERLPHPAIGPITTPLRRVALERLGA
                     LLANGAGRTHSFVAWLQGKPVGALELFVGKEAAGIHGLSVLDEYQGRGVGSALIEHAC
                     AVAAQQGAATMVLLATTEGQRLYAQRGFTEVARFGYWYRSFQRN"
     gene            3602..3946
                     /locus_tag="AC16CY_18695"
     CDS             3602..3946
                     /locus_tag="AC16CY_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3355887.1"
                     /translation="MAVRKGDLLQGTLDLLVLKVLGAGPNHGYGIATRLHQLSDEALR
                     VEEGSLYPALYRMEEQGLIEAEWRPTTNNRTAKFYALTRKGRRAAQAELDGWLRLSGA
                     VTRVLRALEQEA"
     gene            3948..6581
                     /locus_tag="AC16CY_18700"
     CDS             3948..6581
                     /locus_tag="AC16CY_18700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3355888.1"
                     /translation="MALRPPRIIRRLLALFRWHSRDRDMEREMAFHVESLARDYARDG
                     LSDADAQRAARRQFGNLTRLKERGHDERTMRLAEDVARDVRHGARGLWRSPGFSLAVI
                     LTLALGIGGNTAVFSVVDQLLLRPLPYPDGDQIVMVEESVGGRNAHADVSPANWLDWQ
                     RQSRTFRGFAAWGRTQFTLTGAGEPRRLYTQFVSSEFFPLLGVAPLLGRTISEADDRP
                     NGPRVAVLSYRAWQDHLGGDRRAIGRTVQLDERPFEIVGVMPAGFGFVQQDVDLWIAS
                     QLDRNRPWRNTTDGRFLKIVGRLADDATVSSARLEMEGIARRLAATHEFNRNTSVTLT
                     PLRDVLTGEVRTAVLVLFAGVGVLLAIACFNVASMLLARSASRQREIAIRASLGAGRW
                     AIARSMLVESLLLAGAGGALGLGLARWGLDALLAVAPTNLLGVSELFIDRRVLTYAFG
                     VSLATGTIAGLAPTLVFAGRSIADALRTRGSRAGHAPLVRRTLVVVQVAMTVVLLCGA
                     GVLVRTLIALDRAPLGFDARDVLTMVVAISPARYPDERPRDFYREAVTRVRGLPGVES
                     AAVGASTPMVGPPRGGTRFHELGTPERPIRDLPSTVVRMVAPGYFQALRIPVVRGREF
                     SEADNANPMAGFVVNETFARSYLSGRDPLATAITVRMQDENPYLPIIGVVGDVSEGSV
                     RAAPQPTVFYSHGRMPWATMALFVRGRQPESLARPVAAALHELEPTLVISNVRTIETA
                     LAESLARERISAFLSTSFGVGGLLLAALGLYGLLAYLVAERTKDIGIRIALGARLAQI
                     TGSVVAGGLALVAIGAAIGIAGSLLLLRSLGTLVFGVTPYDVPTYAIVVVLLGAIAAV
                     ASYLPARRAARIEPMTALRPE"
BASE COUNT         1093 a         2239 c         2202 g         1138 t
ORIGIN      
        1 cggacgcacg aattccgggc gggcgacgtc tcgtgcagcg acgcccacag ccacctgccc
       61 gagaatgtcg ggagggatac cgagttcgtt caggtccatt tgaagaacag gaagacgttc
      121 gatcatctac agacggggag aagcctcgtc tatcctgctc gagtcaaggc gccggatcca
      181 atcgatgcag atccggcaca ctacttcgtc cagttcgaga acgatgtagt gcggctggcc
      241 cgcatcaagg tccccggagg cgtcaagacc ccgatgcacg cgcatctcgc ctactgcatc
      301 gtcgaaatcc gcgatatgga aaccggagtc aaggccgggg actcatcctg cggcaacgcg
      361 atggctcacg aaagcctgaa caacgccaag agtgtgaatg aagccatcac gatcgaattc
      421 aagaatcgtg acagcttcaa gccctgacgt cactccttct ccagatcccg cggcggcatg
      481 tcggccgggt tgtcggtcca tgcgatctcg ggccacacgt gcacccgcga cttgaagcgc
      541 cagccgttcg cggtcttcac gaacgtgtcc tcgtagccgc cctcccagtg gacgatatcg
      601 ccctgcttgg tggtcttgtt cgtgatcgtg agcagcgtgg acgtcgcctt cgcgccgcca
      661 ggcgcgggcg tgatcaccgg gctgatgacg atgtgatacg ccggatagcg ctgcggcggc
      721 cggcaggagt ccttgccgcc accacccgcg acgcggagct gttcccggcc gcgaaaccag
      781 atcttggcgc cgctgcccca ctgcgacgac acaccgaacg tggcgtcagg agtgaagaga
      841 tccgcgtagg tgttgccgtt gttggtgcag ttgtcgagga cgtgagagta gccgtcgatc
      901 aactggcgaa tgtcgatgta gtcctgggcc tcgagcgccc ccgcggcgcg ggcagtgtcc
      961 tggcttgccg cagcacaccc cgacaatccc gcacacagca gcaccactgc aaggctcgtt
     1021 ggaattcgaa tcatggtttc cccttcggac gcgttgcgta tgccgagtcc acgctgcgcc
     1081 gaattgtagg ccctctcgat ggtggaggcc cccggcttga cggacttctt tgcgagggct
     1141 ctaatcctca cagccttgcc acccatggcg gacatcttca tcagttacgc gcgtgaagac
     1201 gaggaacgga tccggccgct cgctgcggca ctcgaggcgc acggatggtc ggtgttctgg
     1261 gatcggcgaa tcccggctgg tcaaacgtgg cgcagtgttc tcggccagtc gcttcacgac
     1321 gccagatgcg tgatcgtggc ctggtcgcgt cactccgtcg cgtccaggtg ggtcggggag
     1381 gaagctgacg cgggcctgca gcgggggatt ctggttcctg tgctccttga acaggtcgaa
     1441 cccccaatcg gctttcgctc ccttcaggcc gccgagctcg gttccgggcc gctggacgac
     1501 ggttcgccgg cgttcacgca gttcgtgcag gacgtgaaga aagtcctggg cggtccttcg
     1561 tcaccgcaca cggtcccgcc catgcgatcg gtccgcacac taccgtcatc gagccgcgcc
     1621 gccattgcag ggctcgcagt ggttctgctc gcgctcgcgg gcgccgccta cttctattcg
     1681 acgcgggttc cggagccggc aaacggcgag tcggcgacga actcggccaa ttcgtctgcc
     1741 gctggctcgg cgagcaccgg cagcggcatc gcgccgacgc caccggccgt gacaccgtcc
     1801 tccagttccg cggattcgcg agcgggggag ttcgcgatca agatcggcga caaggtgtcc
     1861 gaggacgttc ccggccctgg cgcgggcatc atcgcgcgcc cgttcgagca cgatgtctac
     1921 cggttcacgg ccgcgccccg gcaaatggtc tacttccggg tgcgggagat ctccgcaacc
     1981 cttcaaaaca gttatctggg gtggcgactg gccgacgaag acggcaccga cgtgttcaaa
     2041 caaggcctct acacgggtaa tcccggcgtc cataccttgt tcagaggcgg ggcgtacacg
     2101 ctgaccgtgg ggagccgaga cgacgcggca gtcggccttt acgagtttca gttgtacgac
     2161 gtcccgcgcc ccgatcagtt cgcgatagcg ctcggcacga ggatccggga cggccagccg
     2221 ggacccggag ccggctccat cgagacgccg ggggcgaaag acgtctatac cttcaaggcc
     2281 gcgccgcgtc agcaggtgtc cgctcgtgta ctgggcgggt caccggcgat tctcaacagc
     2341 tatctcggct ggctgctggt ggacgaggac ggcaccgaga tattcaagcg ggggctgtac
     2401 accggtcacc cgggcgtgct cacgttgatc agggggggaa cctacacgtt gaccgtcggg
     2461 agcgacgtcg atcccgccac tggcacctac gatgttcaaa tcgatgccca gaactgacta
     2521 gcaccccgac acacgtccag aaaagcatcg cgggcgtcgg gctcgcgtca tgcggtcaat
     2581 tccgttggaa actgcggtac cagtatccga agcgggcaac ttcggtgaaa ccgcgttgcg
     2641 cataaagacg ctggccctcg gtcgtcgcca gcaacaccat cgtcgctgca ccctgctgcg
     2701 cggcaacggc gcacgcgtgc tcgatgagtg ccgatccgac accccggccc tgatactcgt
     2761 cgagcaccga caggccgtga atgcccgcgg cttccttgcc gacgaagagt tcgagcgcac
     2821 ccactggttt gccctgaagc cacgcgacga acgagtgtgt gcgtccggcg ccgttcgcca
     2881 gtaaggcccc tagtcgttcg agcgcgacgc gtcggagcgg cgtcgtaatc ggacctatcg
     2941 caggatgagg aaggcgctcg tactcggcgg cgtccgtcac cggccgaatc tccagggcgg
     3001 cagcacgcga acggacgggc acgtgctcgg caagatggcg caccatcgcc gggtagtact
     3061 tccgacaccg cagtccgcgg tcgtgaagta gagcaggcag attgtcaggc gtcgcggacg
     3121 gcgaaatcca cagggccatt ccacgacgat gacgctcgta ccggcggacg agcgtatcga
     3181 gccgttgtcc ggctccacca gccgggaacc gcaccatgat gccggcgttc cgccacgcct
     3241 gaccgccatg aacgatccag acgacatctc gatcccgatg cacctcgaca cccggaacga
     3301 cacgctcccg cagaaaactc tccgcaaggt gtcgctcgac gagcagggac tgtgtcaggt
     3361 ccacagggct cgcatcgcga catcgcgggt cgcagcgcac ggttctcaca ccaaccatgt
     3421 cgcacattcg agcgtgcgac acaagcatcg tccgaattgc ctcgtgtccc gtcttccctt
     3481 gaccgtctaa gtatgactgt gttctactga cactcaccca gacggtctaa gcgagccgcg
     3541 tcggggcctg cccgccgtag ctcaatgaat caggtgagcg aaggtgggag gagccgaatc
     3601 gatggcggtg cggaaaggcg atctgctgca gggcacgctg gatcttctcg tgctgaaagt
     3661 gctgggggcc ggccccaatc acggctacgg catcgcgacg cggctgcatc agctctccga
     3721 cgaggcgctg cgagtggagg agggctcgct ctatccggcg ctctatcgca tggaagaaca
     3781 gggcctcatc gaggccgaat ggcgccccac cacgaacaac cgcaccgcca agttctacgc
     3841 gctgacgcgg aagggacgcc gcgcggcgca ggccgagctc gacggctggc tgcggctgtc
     3901 cggcgccgtc acgcgcgtgc tgcgcgcgct cgagcaggag gcgtgacgtg gccctgcgac
     3961 cgccgcgcat cattcgccgc ctcctcgcat tgttccgatg gcactcgagg gatcgcgaca
     4021 tggagcgcga gatggcgttc catgtcgagt cgctcgcgcg cgactacgcg cgtgacgggc
     4081 tgagcgacgc cgacgcgcaa cgcgccgccc ggcggcagtt cggcaacctg acgcggctca
     4141 aggagcgcgg ccacgacgag cggacgatgc gccttgccga agatgtcgcg cgggacgtca
     4201 gacacggggc tcgcggtctc tggcgcagcc cgggcttctc gctcgccgtg atcctgacgc
     4261 tggccctcgg catcgggggc aacaccgccg tcttctccgt cgtcgatcag ctcctgctgc
     4321 ggccgctgcc gtacccggac ggcgaccaga tcgtgatggt cgaagagtcg gttggcggtc
     4381 gcaacgcgca cgccgacgtg tcgcccgcca actggctcga ctggcagcgc cagagccgca
     4441 cgtttcgagg atttgccgcc tgggggcgga cgcagttcac gctcaccggt gctggcgagc
     4501 cgcggcgcct gtacacgcag ttcgtgtcgt ccgagttctt tccgttgctc ggcgttgcac
     4561 cgctcctcgg ccgcacgatc tccgaggcgg atgatcgccc gaacggcccg cgcgtcgccg
     4621 tgctcagcta ccgcgcctgg caagatcacc tgggcggcga tcggcgcgcc atcggccgca
     4681 cggtgcagct cgacgagcgc ccgttcgaaa tcgtcggcgt catgcctgcg ggctttggct
     4741 tcgtgcagca ggatgtcgac ctgtggatcg catctcagct cgatcgcaac cgtccgtggc
     4801 ggaacacgac agacggacgc ttcctcaaaa tcgtcggacg cctcgcggac gacgcgaccg
     4861 tcagttcggc ccgattggag atggagggga tcgcgcggcg tcttgccgcg acgcacgagt
     4921 tcaacaggaa cacgagcgtg acgctgacac ccctgcgcga cgtactcacg ggcgaggtgc
     4981 ggacggcggt gctcgtgctc ttcgccggcg tgggcgtgct gctcgccatt gcctgcttca
     5041 acgtcgccag catgctcctg gcgcgatcgg cgtcgcgcca gcgcgaaatc gcgattcgcg
     5101 cctcactcgg ggcgggacga tgggcgattg cgcgatcgat gctggtcgaa agcctgctgc
     5161 tcgccggcgc cggcggcgcg ctcggcctcg ggctcgcgcg ctggggtctg gacgcgctgc
     5221 tcgccgtggc ccccacgaac ctgctcggcg tctccgagct gttcatcgac cggcgcgtcc
     5281 tgacgtatgc gttcggcgtg tcgctcgcga caggcacaat tgccggcctc gcgccgacgc
     5341 tcgtgttcgc ggggcgatcg atcgccgatg cgttgcgcac gcgtggctcg agggccggtc
     5401 acgcgcccct ggtgcgacgg acgctcgtcg tggttcaagt cgccatgacc gtcgtgctct
     5461 tgtgcggcgc cggtgtcctc gtgcgaacgc tcatcgcgct ggatcgggcc cccctgggat
     5521 tcgacgcccg cgacgtgctc acgatggtgg tcgcaatttc gccggcgcgt tatcccgacg
     5581 agcgcccgcg cgacttctat cgcgaggcgg tgacgcgcgt tcgcgggctg ccgggcgttg
     5641 aatccgcggc cgtgggggcc agcacgccga tggtcggtcc tcctcgtggc ggaacacggt
     5701 ttcatgagct cggcacgccg gagcgcccga tccgcgacct gccgtccacc gtcgtgcgga
     5761 tggtcgcgcc cggatacttc caggcgcttc gcattcccgt ggtgcgtgga cgcgaattca
     5821 gcgaggcgga caacgcgaat ccgatggccg gattcgtcgt caacgagacg tttgcgcgaa
     5881 gctacctctc gggacgcgat cccctggcga cggccatcac cgtgcgcatg caggacgaga
     5941 atccctacct gccgatcatc ggcgtcgtcg gcgacgtgag cgaaggctcg gtgagggccg
     6001 cgccgcagcc gaccgtgttc tacagtcacg gccgcatgcc gtgggcgacg atggcgttgt
     6061 tcgtgcgcgg ccggcaaccg gagtcgctcg ccaggccggt cgccgcggcg ctgcacgagc
     6121 tcgagccgac gctggtgatc tcgaacgtcc gcacgatcga aaccgcgttg gcagagagcc
     6181 tggcgcgcga acgcatcagc gcgttcctct cgaccagctt cggcgtcggg ggcctgttgc
     6241 tggcggcgct cggactctac ggattgctcg cgtatctcgt cgctgagcgg acgaaggaca
     6301 tcggcatccg catcgccctt ggcgcgcggt tggcccagat cacgggatcg gttgtcgccg
     6361 gcggcctcgc gctcgtcgcc atcggcgcgg caatcggcat cgccggctcg ctgctgctcc
     6421 tgcgctcgct cggcacgctg gtcttcggcg tcacgccgta cgacgtcccg acgtacgcga
     6481 tcgtcgtcgt gctgctgggc gcgatcgccg cggtggcgtc ctacctgccc gcgcgccgcg
     6541 ccgcgcgcat cgagccgatg acggcgctca gacctgagta gaaggaaatc cacacggagc
     6601 tgggtttcag gcggcactgc ctcgcattct ggacagttgc gaccctaccg caacgaacca
     6661 tcccccaaag ga
//
LOCUS       DBNGSH010000814         5500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_603056, whole genome shotgun sequence.
ACCESSION   DBNGSH010000814 DBNGSH010000000
VERSION     DBNGSH010000814.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_603056"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="AC16CY_18705"
     CDS             <1..358
                     /locus_tag="AC16CY_18705"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR3355889.1"
                     /translation="ARVVHHAELRARLAPALRSRPRAYWIEILTEAGVPSGAVRDVDE
                     VLADPQVLARDMVTAVEHATEGTLKVLGVPIKLSETPGSVRTAPPTLGQHTTAVLREL
                     GVEDGEIQHLRRERVI"
     gene            496..924
                     /locus_tag="AC16CY_18710"
     CDS             496..924
                     /locus_tag="AC16CY_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355890.1"
                     /translation="METSAVRRRVQAAMAAARERARQRRARVDEADKIYGDFLERVAA
                     PVSRQIAQALKAENYPFTVSTPGRGVRLSFEHGRDDYVELGLQTGTDEPQLVGRIRRT
                     RGSHTIDEELPVKPGTPPQDLSEEDVLEFLMRALEPWLER"
     gene            complement(1042..1464)
                     /locus_tag="AC16CY_18715"
     CDS             complement(1042..1464)
                     /locus_tag="AC16CY_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3355891.1"
                     /translation="MYRKILVAVENSGADRTILTHVRQLAALTGAEILLVHVADGWAA
                     RHFDQLKLRESEEMKIDRAYLDQLATELRAGSLTVATELAMGDPATELIRLAEERKVD
                     LIAMSTHGHRFFNDLLRGTTVTKVRHVVKIPILLLRAQ"
     gene            complement(1467..2765)
                     /locus_tag="AC16CY_18720"
     CDS             complement(1467..2765)
                     /locus_tag="AC16CY_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019422989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR3355892.1"
                     /translation="MPNAPSLREVHGSIAVPADAGFWRKLFAFAGPGYLVAVGYMDPG
                     NWATDLAGGARYGYTLLSVILISNLMAILLQALAVRLGIASGRDLAQACRDSYSKPVT
                     IVLWVLCEIAIAACDLAEVIGAAIALNLLFGLPLIWGVSLTALDVLLILFLQHRGFRY
                     VEALVVALIVAIAGSFAIQIWLARPDALALAVGFVPRSEIVTNPAMLYIALGILGATV
                     MPHNLYLHSSIVQTRQYVDTIASKKEAIRFATLDSSIALMSALFINAAILIVSAAVFH
                     GTPHESVADIGDAHQLLSPLLGTSAAGILFAVALLCSGQNATLTGTLAGQIVMEGFVN
                     IRLRPWLRRLITRLVAIVPAVIVVSLYGEQGTGALLVLSQVILSLQLPFAVFPLVSFT
                     SNRHKMGAFVAPRWVQILAWTVAVIIAGLNVWLLFQTFVA"
     gene            2948..4714
                     /locus_tag="AC16CY_18725"
     CDS             2948..4714
                     /locus_tag="AC16CY_18725"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HIR3355893.1"
                     /translation="MPSSTVENYLKAIYTGVASLDPPQRLLPMGQLASALDVAPGTAT
                     TMVKTLAESGLVRYEPYSGVALTAAGQKLAALVLRRHRLIELFLVQVMGYSWDEVHDE
                     AEQLEHVVTDRLVDRIDEMLGRPEVDPHGDPIPTADGLVRTKKMQTLLTCPLATTVTV
                     TRVIDQDRKFLRFIEQHNLKPGQSIEVEARDPAADSVRVRGSNNKRLTIGTRAASKLL
                     VHVASILVLLAAPSQAQTTQSSEPPRTALSGYMEAHYNNAEFEDGRLDFHRFVLLVTH
                     SFTDRIRFVGELELEHALVEGLEDAGELELEQAYIDFLISRSFNVRAGMMLMPIGIIN
                     ERHEPPVFYGVERPFNDTVIIPTTWFEVGAGVHGEIGRGWRYRAFVVAPLNAAEFDAD
                     EGLRGGRQRGSESNIRRPGVTGRLEYVGVRGLTVGASGWSGRSGFEFQPTFDVPVSLA
                     EADARYARGRLELRGQVSQVWIGNAGQLNDALGLRIGVNPNIAEAIGGFYLEAGYRAI
                     SRPRFGEVGTFVRYEDFNTQRRMPPGYVALPEFDRNAWVVGATYWPDPDVAIKVDYTV
                     VGSKSTVVQAPNSFNAGLGWWF"
     gene            4714..5148
                     /locus_tag="AC16CY_18730"
     CDS             4714..5148
                     /locus_tag="AC16CY_18730"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3355894.1"
                     /translation="METQGAGGRGRGAGGRGQGAGGKAAALALVAVVSCVAAVAAVWR
                     LDLAAQQQAQVIEINAERFSFYPSEIKVKAGTRLEIRLTSEDTMHGFRIIGTDVDVEL
                     PKRGRGVATVMFQPAAGRYTFECSRLCGAGHSFMRGIIIAAE"
     gene            5157..>5500
                     /locus_tag="AC16CY_18735"
     CDS             5157..>5500
                     /locus_tag="AC16CY_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355895.1"
                     /translation="MTPTYVVSGFLGRRSARREGGSRTGVALGAIALLGGLLVAQVRT
                     PLPGDPLPGVTPVEFEEFRLGLDDFLEVETAEEGLGPAFNGASCAVCHSVPAVGGTGS
                     IAEVRAAKRGSRG"
BASE COUNT          964 a         1786 c         1902 g          848 t
ORIGIN      
        1 cgctcgcgtc gtccaccacg ccgagctgcg cgcgcgcctt gcgccggcgc tccgatcccg
       61 gccgcgggcg tactggatcg aaatcctgac ggaggcgggc gtgccctcgg gcgcggtgcg
      121 agacgtcgat gaggtgcttg ccgatccgca ggtgctcgcg cgggacatgg tgacggccgt
      181 cgagcatgcg accgagggta cgctcaaggt cctgggcgtt cccatcaagc tgtcggagac
      241 gccgggctcg gtgcgcacgg cgcctccaac gctcgggcag catacaaccg cggtgctgcg
      301 cgagctgggt gtcgaagacg gggagatcca gcatcttcga cgcgaaaggg tgatttgacg
      361 cgacgcggcg gctgaagccg ccgctctacg atcgattggc tttgcggcag ccgtacgcca
      421 tcgtagagcg cacgcttcag cgtgcgcggt agtagctgac acgaacgttc actacagaga
      481 cgaagaggac acagagtgga aacatcagcc gttcggcgcc gcgttcaggc cgcgatggca
      541 gcggcgcgcg agcgggcgcg gcagcgccgg gccagagttg acgaggccga caagatctac
      601 ggggatttcc tcgagcgcgt ggcggcgccc gtgtcgcgtc agatcgccca ggccctcaag
      661 gcggagaact atccgttcac ggtgtccacg ccgggccgtg gagtccggtt gtcgttcgag
      721 cacggacgtg acgattacgt cgagctcggc ttgcagaccg gaacggacga gcctcagctc
      781 gtgggccgca tccgccggac gcgcggctcg cacaccatcg acgaggaact gccggtcaag
      841 ccgggcacgc cgcctcagga tttgagcgag gaggacgtac tggagttcct gatgcgggcg
      901 ctcgagccct ggctggagcg gtaacttaac tcccaacccc caacacccaa ctcccaatcg
      961 cttcccgaat tcagaaagtt gggagttgga ggttgggagt tgggagtttg cgcgcgcaat
     1021 gacgcgctgc ttcaaccggc gtcattgtgc gcgcagaagc aggatcggga tcttcaccac
     1081 gtgacggacc ttcgtcaccg tggtgccccg cagcaggtcg ttgaagaagc gatgtccgtg
     1141 ggtggacatc gcgatgagat cgaccttgcg ctcttcggcc aggcgaatga gctcggtggc
     1201 cggatctccc atcgccagct cggtcgccac cgtgagcgac cccgccctca gctccgtcgc
     1261 gagctggtcg aggtaggcgc ggtcgatctt catctcctct gactcgcgca gcttcaactg
     1321 gtcgaaatga cgggcggccc agccgtcagc gacgtgaacc aggaggattt cggcgcccgt
     1381 gagcgccgcg agctggcgca cgtgcgtcag gatggtgcga tcggcgccgg aattttcgac
     1441 ggcaacgaga atcttgcggt acacggctat gcgacaaacg tctggaacaa cagccagaca
     1501 ttgagccccg caatgataac ggcaacggtc caggcgagga tctgtaccca ccgcggcgca
     1561 acgaaggcgc ccatcttgtg cctgttgctc gtgaagctca cgagaggaaa gacggcgaac
     1621 ggcagctgca ggctgaggat gacctggctg agcaccagga gtgctcccgt cccctgctcg
     1681 ccgtagagac tcaccacgat caccgccggg acaatcgcca cgaggcgcgt gatgaggcgc
     1741 cgcagccacg gccggagccg gatgttgacg aaaccctcca tcacgatctg ccccgcgagc
     1801 gtgccggtca gcgtcgcgtt ctggcccgag cagagcagcg ccaccgcgaa caggattccg
     1861 gcggcgctcg tgccgagcag cggcgacagg agctggtgcg catcgccgat gtcggcgacc
     1921 gactcgtgcg gcgtcccatg gaacacggcc gccgagacga tgaggatcgc ggcattgatg
     1981 aagagcgccg acatcagcgc gatcgacgag tcgagggtgg cgaaccggat cgcctccttc
     2041 ttgctcgcta tcgtgtccac gtactgccgc gtctgcacga tcgacgagtg caggtagaga
     2101 ttgtgcggca tgaccgtcgc gccgaggatg ccgagcgcga tgtacagcat cgccgggttc
     2161 gtcacaatct cgctgcgcgg cacgaagccg accgccaggg cgagcgcgtc cggccgggcg
     2221 agccagatct ggatcgcgaa cgacccggcg atggcgacga tgagcgcgac cacgagcgct
     2281 tcgacgtagc ggaagcccct gtgctgcagg aagaggatca ggagcacgtc cagcgccgtc
     2341 agcgagaccc cccagatgag cggcaggccg aacagcaggt tgagggcgat ggcggcgccg
     2401 atcacctcgg cgaggtcgca cgcggcgatg gcgatttcgc agagcaccca caggacgatc
     2461 gtgaccggct tggagtagct gtcgcggcag gcctgcgcga gatcgcggcc gctggcgatg
     2521 ccgagcctca cggcgagcgc ctgcagcagg atcgccatga ggttcgagat caggatgacg
     2581 ctcaggagcg tgtaaccgta gcgcgcgccg ccggcaaggt cggtcgccca gttgcccggg
     2641 tccatgtagc cgacggcgac gaggtagccg ggccccgcaa acgcgaagag cttccgccag
     2701 aagcccgcat ccgcgggcac ggcgatcgag ccgtggacct cgcgcaggct cggggcgttg
     2761 ggcacgctcc gccacccgga ggcagacccc gccgccggaa ccactggctc cacggccgcg
     2821 tcgggaacag tgtgattttg aatagtcaaa aaaatatctt gttctgcttc aaaagtcaaa
     2881 ctacactccc ggggtcagat cttgattttt gcgcccttgc aaaaaagaga tctgacccca
     2941 ggacgccatg ccgtcgagca ccgtcgagaa ctacctcaag gcgatctaca ccggggtcgc
     3001 cagcctcgac ccgccccaac gcctgctccc gatggggcag ctcgcctccg ccctcgacgt
     3061 cgcccccggc acggcgacga cgatggtcaa gacgctggcg gagtcgggcc tcgtcagata
     3121 cgaaccctac tccggcgtcg cgctcacggc ggccggacag aagctggccg cgctcgtcct
     3181 gcggcggcat cggcttatcg agctcttcct cgtgcaggtc atggggtact cctgggacga
     3241 agtccacgac gaggccgaac agctcgagca cgtggtgacc gatcgcctcg tcgaccggat
     3301 cgacgagatg ctcgggcgcc ccgaggtcga tccccacggc gatccgattc cgaccgccga
     3361 cggcctcgtc aggacaaaga agatgcagac gctcctcacc tgccccctcg ccacgacggt
     3421 caccgtgacg cgcgtcatcg atcaggacag gaagttcctg cgcttcatcg aacagcacaa
     3481 cctcaagccc ggacagtcga tcgaagtgga agcgcgcgac ccggcggccg acagcgtccg
     3541 cgtgcgcggc agcaacaaca agcggctgac gatcgggacg cgggccgcgt ccaagctgct
     3601 cgtccacgtg gcgtctatcc tcgtgctcct ggccgcgccc tcgcaggcgc agaccacgca
     3661 gtcgtcggaa ccgccgcgca cggcgctctc gggctacatg gaggcgcact acaacaacgc
     3721 cgagttcgag gacggccgcc tcgacttcca ccgcttcgtc ctcctcgtca cccactcctt
     3781 caccgatcgc atccgtttcg tgggagagct ggagctcgag cacgccctcg tcgaggggct
     3841 ggaagacgcc ggcgagctgg agctcgagca ggcgtacatc gactttctga tctcccgttc
     3901 cttcaacgtc cgcgcgggga tgatgctgat gccgatcggc atcatcaacg aacggcacga
     3961 gccgccggtc ttctacggcg tggagcgccc cttcaacgac acggtcatca tcccgacgac
     4021 ctggttcgag gtgggcgccg gcgtgcacgg ggagatcggg cggggctggc gctaccgcgc
     4081 gttcgtagtg gcaccgctga atgccgctga attcgacgcc gacgaaggcc tccgcggcgg
     4141 gaggcagagg ggctcggagt cgaacatcag gcgcccgggc gtcaccggcc ggctcgagta
     4201 cgtcggcgtg cgcggcctca ctgtcggcgc gagcgggtgg agcggacgct ccggtttcga
     4261 gttccagccg accttcgacg tgccggtctc gctggccgag gccgatgccc ggtatgcacg
     4321 cggccgtctc gagctgcggg gccaggtctc gcaggtctgg atcggcaacg ccggccagtt
     4381 gaacgatgcg ctggggctca gaatcggcgt caacccgaac atcgccgagg cgatcggcgg
     4441 cttctatctc gaggcggggt accgggcgat ctcgaggcct cgctttggcg aggtcggcac
     4501 gttcgtcagg tacgaggact tcaacacgca gcggcgcatg ccgcccggct acgtggcgct
     4561 tcccgagttc gatcgcaacg cgtgggtcgt cggcgcgacc tactggccgg atcccgacgt
     4621 cgcgatcaag gtcgattaca cggtcgttgg aagcaagagc acggtcgtcc aggcgcccaa
     4681 ctcgttcaac gcggggctgg ggtggtggtt ctgatggaaa cacagggggc agggggcagg
     4741 gggcgggggg cggggggcag ggggcagggg gcagggggaa aggccgctgc gctggcgctc
     4801 gtcgcagtgg tatcgtgcgt ggccgctgtc gccgctgtct ggcgcctcga tctggcggcg
     4861 cagcagcaag cgcaggtcat cgagatcaac gccgagcgct tcagcttcta tccgtccgag
     4921 atcaaggtca aggccggcac acgcctcgag atccggctca ccagcgagga caccatgcac
     4981 ggcttccgca tcatcggcac cgatgtcgac gtcgagctgc cgaagcgggg acgaggtgtc
     5041 gcgacggtca tgttccagcc ggccgcggga cgctacacgt tcgagtgttc gaggctgtgc
     5101 ggcgccgggc attcgttcat gcgcggcatc atcatcgccg cggaatgaga ggggtcgtga
     5161 ctccgacgta cgtagtgtcc ggctttcttg ggcgccgaag cgccaggcgc gaaggcggca
     5221 gccggaccgg cgtggccttg ggcgcaatcg cattgctggg cggcctgctc gtcgcgcagg
     5281 ttcgtacccc gctgcccggg gatccgctgc ccggtgtcac gcccgtcgag ttcgaggagt
     5341 tccggttagg cctggacgac tttctcgagg tggaaaccgc cgaagaaggg ctcggcccgg
     5401 ccttcaacgg cgcgagctgc gccgtgtgcc acagcgtgcc ggcggtcggc ggcacgggct
     5461 cgattgccga ggtgcgggcg gcaaagcgcg gatcgcgcgg
//
LOCUS       DBNGSH010000815         1621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_943762, whole genome shotgun sequence.
ACCESSION   DBNGSH010000815 DBNGSH010000000
VERSION     DBNGSH010000815.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_943762"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="AC16CY_18740"
     CDS             complement(<1..335)
                     /locus_tag="AC16CY_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355896.1"
                     /translation="MPGLILDGALTRIPETYRRLGAALGVAERGERLAAARLRANPAW
                     QSVGAVVAGRVYQYPGLPYSWGARPPSVNRMPGLVWLAYALSGRPFDAQFDADIRGFF
                     SDFYHVELT"
     gene            635..1597
                     /locus_tag="AC16CY_18745"
     CDS             635..1597
                     /locus_tag="AC16CY_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017292147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR3355897.1"
                     /translation="MEASLGIDIAKAKFAVALLTADGKVRHKSCVNTPAGFTDLGTWL
                     HRQRVARVHACLEATGTYGEALATWLHDAGHTVSVVNPAIIHAYARTQLARTKTDRLD
                     AELIAHFTATQQPPAWTPPAPEIRQLQALVRRLDALQGMRTQELNRLEAGGAVGAVRA
                     SIEAVLAHLDTQIALVKQLIRDHLDRHPGLRAQRELLITIPGIGETTAAVLIAELFDK
                     SYTSARQAAAFAGLVPRIIESGTLRGRSRLSKLGPGRLRKALYLPAVAALRWNPTIRA
                     VRARLHAAGKPPMVIIGAAMRKLIHLAYGVLKSGKAYEPTCAHP"
BASE COUNT          263 a          563 c          532 g          263 t
ORIGIN      
        1 tcggtgagct ccacgtggta gaagtcgctg aagaaaccgc ggatgtcggc gtcgaactgc
       61 gcgtcgaacg ggcgccctga aagcgcgtac gcgagccaga cgaggccagg cattcgattg
      121 accgacggcg ggcgtgctcc ccagctgtac gggagcccgg gatactgata cacgcgaccc
      181 gccacgaccg cgccgaccga ttgccatgcc ggattggcac gcagccgcgc ggcggcaagg
      241 cgctctccgc gctcggcgac gccgagtgcc gcgccgaggc ggcggtacgt ctccggaatc
      301 cgcgtcagcg ccccgtcgag aatgaggccc ggcactcgcg tgcgccgctg tcgcgccgcc
      361 gctccctcgt ggctgtgtcg cttcgtattc ttcgtgcctt gtatgttgcg ttcactgctt
      421 gactgcgttc cagtccggcg gtctactcgg ggacgcctga gtaggagcgt cggggtgctg
      481 tttcgtgcct gaggccatgc gagcctgtca tcgaagatca gcatccgacg ctcaccgtgt
      541 ggcgtccggt cgaacggtat cgaatgcggg cgcgtcgcgt ccagcatgta tgcacaaggc
      601 tgaccgcaca cgacgccacc gaaaggagcg acgtgtggag gccagtctgg gaatcgacat
      661 cgccaaagcg aagttcgcgg ttgcgttgct gaccgcggac ggcaaagtcc gtcataagag
      721 ctgcgtgaat acgccggctg ggtttaccga cctcggcacc tggctgcacc ggcagcgcgt
      781 cgcccgggtc cacgcgtgtc tggaagccac cggcacgtac ggggaggcgc tggcgacatg
      841 gctgcacgac gcggggcaca ccgtcagcgt cgtcaatccc gcgatcattc acgcctatgc
      901 gcgcacgcag ctggcgcgca cgaagaccga tcggctcgac gccgagctca tcgcccactt
      961 caccgccacc cagcagccgc cggcgtggac gcccccggcg ccggagattc gccagctgca
     1021 ggcgctcgtg cgccgcctcg acgccttgca gggcatgcgg acacaggaac tgaatcggct
     1081 cgaggccggc ggggcggtgg gggccgtccg ggcctcgatc gaggccgtgc tcgctcacct
     1141 cgacacgcaa atcgcgctcg tcaagcagtt gattcgtgat catctcgatc ggcatccggg
     1201 cctgcgcgcg cagcgcgagc tgctgattac cattcccggc atcggcgaga ccaccgcggc
     1261 ggtcctcatc gccgagctct tcgacaagtc ctacacgagc gcgcgccagg ccgccgcctt
     1321 cgccggcctc gtgccgcgga tcattgagtc cggcacgctc cgtgggcgga gccggctgtc
     1381 caagctcggg ccgggccgcc tgcgcaaagc gctctatttg ccggcggtcg ccgcgctccg
     1441 gtggaatccc acgatccgcg ccgtgcgcgc acgcctgcac gccgctggca aaccaccgat
     1501 ggtcatcatc ggcgccgcga tgcgtaaact cattcatctc gcctatggcg tactcaaatc
     1561 gggaaaagcg tacgaaccga cgtgcgccca cccttgactt tcaacacggt atcttcgtgg
     1621 c
//
LOCUS       DBNGSH010000816         4322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2302637, whole genome shotgun sequence.
ACCESSION   DBNGSH010000816 DBNGSH010000000
VERSION     DBNGSH010000816.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2302637"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="AC16CY_18750"
     CDS             <1..960
                     /locus_tag="AC16CY_18750"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3355898.1"
                     /translation="GVPAVRAGGLQGLMDVVLHPRFAENRWVYLAYHKPVTVPGQTTP
                     QGGPVTEGATTLARGVWNGTALTGVKDIFESGATRTESSRIGFGRDGMLYMTISASGT
                     GPDVFRSADPNDYAGKTVRLRDDGTVPPDNPFAGKPGYKPGIYTLGHRNGHSMSLNPE
                     TGEFWVTEQGPNGGDEINILKPGADYGWPQTSHGRNYMGPRISESPYREGTQQPVVVW
                     VPSIAVAGGTFYTGDVFAGWKRSYFVGGLREGETPRTGQMQRIEFNEKWEELRREPML
                     RELKQRIRDVRQGPDGLLYVVTGEAQGALLRIEPAGAPASGAR"
     gene            1178..2146
                     /locus_tag="AC16CY_18755"
     CDS             1178..2146
                     /locus_tag="AC16CY_18755"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3355899.1"
                     /translation="MRTRISGAFERVNKELWVILSLFAIALLLNQVIAAQRMVLSFFV
                     LPTVASSYFYGRRHGTLTAFASVLLVVGLVLFRSSLPGGSAVATLSYLDWLELTVWGG
                     SLIVTGYLMGTLYEHKNQQIEELRETYQGVLMILRHFISKDTYTENHCYRVSVYATRI
                     AAQLNLPNDRIEDVRAAALLHDIGKLEISRDLLYKAARLTKEEYEKVQEHVSRGIDIL
                     EPVGGALRRVIPIILAHHDKFDGSGYNPTSGESIPFEARIIAVADVYDALTSDRPYRK
                     AMSTFEARDVICNGENTEFDPKVVAAFNALFRRGELEIWSAPLSAA"
     gene            2298..2435
                     /locus_tag="AC16CY_18760"
     CDS             2298..2435
                     /locus_tag="AC16CY_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355900.1"
                     /translation="MMNETVYLFALSLPALLAPIVADPDDGRAPALPVTEEQVAEAAW
                     I"
     gene            2426..2644
                     /locus_tag="AC16CY_18765"
     CDS             2426..2644
                     /locus_tag="AC16CY_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIR3355901.1"
                     /translation="MDLNRRDTARRAEVVRSVLEEIVHSGVPVITIEGLREALHVSPE
                     AATRIVERLVSAGLLVEVRSGVWTRVTT"
     gene            2659..2889
                     /locus_tag="AC16CY_18770"
     CDS             2659..2889
                     /locus_tag="AC16CY_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355902.1"
                     /translation="MGLLVLSLVLLLTIALGIALAKGVLNLILHLLTEQRVPAVDAFR
                     VAVFVGALIVFWSLAPAIAESPAASGLNAFMR"
     gene            complement(2920..>4322)
                     /locus_tag="AC16CY_18775"
     CDS             complement(2920..>4322)
                     /locus_tag="AC16CY_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate synthase"
                     /protein_id="HIR3355903.1"
                     /translation="GEMNQWAKDFFGRPIISDWRTQLDFTTKIFRARGLHLDDRHVRR
                     ADGGGFSASIVDMALYVVNNERQMRETGASLVLYLPKIQTAEEAALWGEMIGALERHL
                     GLPDGRIKVYVLVEQLEAVYQLMEIRAALGSHFVGFNTGRWDYINSVADAMAWDPAFV
                     DPNIDAITMTYGYMRNYEDRVRRAVNTPDSRGQYALWQGGMEPNIPVGSPDGVKRAME
                     RAVAGAEREEREGASGKWVAHWKMVHIVRPVWERAGKANQLGRPFPRLTYAQEDADGL
                     LLLEPAPRTIRGARDLLSVALQYGNAFGQGMQAAALKPADFFGNDDVLYLMEDMATGE
                     IRLSILWEWLHKGARLTADDAETGLKSGDVFSAPIFARLLSEEYAKLRRAGNRDVHDN
                     SKETTLPIAREIVEAYVTDEVKLPWYIDLLNINLGVHDVAEGKRRIELLRAAFKADGK
                     RVTANLDFDAEQASGI"
BASE COUNT          734 a         1429 c         1382 g          777 t
ORIGIN      
        1 ggcgttcccg cggtgcgcgc agggggactg cagggactga tggacgtggt gctgcacccc
       61 cgcttcgcgg aaaaccgctg ggtgtacctg gcgtaccaca agcccgtcac ggtgccgggc
      121 cagacgacgc cacagggcgg acccgtgacc gaaggcgcga cgacgctcgc tcgcggcgtc
      181 tggaatggaa cggcgctgac gggcgtcaag gacatcttcg aatcgggcgc cacgcgcacc
      241 gagtcctcgc gcatcgggtt cgggcgcgac gggatgctct acatgacgat cagcgcgtcg
      301 ggcacgggcc cggacgtctt ccggtccgcc gatcccaacg actatgccgg caagaccgtg
      361 aggctgcgcg acgacggcac cgtcccaccg gacaacccgt tcgccggaaa acccggatac
      421 aagccgggga tttacacgct cggacacagg aacggccact cgatgtcgct gaaccctgaa
      481 accggggagt tctgggtgac cgagcaggga ccgaacggcg gagacgagat caacatcctg
      541 aagcctggcg ccgactacgg atggcctcaa accagccacg gccggaacta catggggccc
      601 aggatctccg agagccctta ccgggaaggc acccagcagc cggtcgtcgt gtgggttccc
      661 tctatcgccg tcgccggggg taccttctac acgggcgatg tcttcgcggg atggaagcga
      721 agttacttcg tcgggggttt gagagaaggt gaaactccgc gcacgggtca gatgcagcga
      781 atcgagttca acgagaaatg ggaggagctc aggcgcgagc cgatgctccg ggaactgaag
      841 cagcggatcc gcgatgtccg gcaagggcct gacggcctcc tgtacgtggt gacgggcgag
      901 gcgcagggtg ccctgctccg gatcgaacca gccggtgccc cagcctccgg agcgcgctag
      961 agccagaaat cgactttgcc gcggaagaaa acggggggtc gtgttccggt ccggcacagt
     1021 ccggcaccag cgccagcttc ccatcgtttc cagagactga accgcaactc tctgcgttcc
     1081 agcgcgttac cggcagcagt gcgtgatttt ccccgccgga acgacagttg ctccatagcg
     1141 gccgatgcct ccttcgaggc ggtgttgaag ctccgttatg agaacacgca tctccggcgc
     1201 gtttgagcgg gtcaacaagg aactgtgggt catcctttcg ctgtttgcga tcgccctgct
     1261 tctgaatcag gtgatcgccg cgcagcgcat ggtcctgagc ttcttcgtgc tccccaccgt
     1321 tgcctcatcg tatttctacg gacgccggca cgggacgctg accgcctttg cgagcgtgct
     1381 cctggtggtg ggtctggtgc tgttccgcag ctccctgccg ggagggtcgg cggtcgcgac
     1441 cctcagctac ctggattggc tggaattgac ggtctggggc ggctcgctca tcgtcacggg
     1501 gtatctgatg ggcaccctgt acgaacacaa gaaccagcag atcgaggaac tgcgggagac
     1561 ctatcagggc gtgctgatga tccttcggca cttcatctcg aaggacacct acacggagaa
     1621 ccactgttac cgtgtgtcgg tctatgcaac ccggattgcg gcgcagctca accttccgaa
     1681 cgatcgcatc gaggacgtgc gggcggcggc gttgcttcac gacatcggca agctcgagat
     1741 cagccgcgat ctgttgtaca aggccgcgcg gctcacgaaa gaggaatacg agaaggttca
     1801 ggagcacgtg tcccgcggga ttgacatcct cgagccggtg ggcggggctt tgcgccgggt
     1861 catcccgatc atcctggccc atcacgacaa gttcgacggg agcggctaca accccaccag
     1921 cggcgagtcc attccgttcg aggcgcgcat cattgccgtc gccgacgtct acgacgccct
     1981 gacgagcgac cgtccgtatc gcaaggcgat gtccaccttc gaggcgcgcg acgtcatctg
     2041 caacggcgaa aatacggagt tcgacccgaa ggtcgtggcc gcgttcaacg ctctgttccg
     2101 ccggggagag ctggagatct ggtcggcgcc gctgtcggcg gcctgaatcc ggggcacccc
     2161 gcaccctcgc ccctcgcacc ctcgcaccct cgtaccctcg caccttcgaa ccttcgcacc
     2221 ccctccccct attgcccagt ctggctcgac cgataacccc acgggagagg attgcccttg
     2281 cccagggggg cccgccagtg atgaacgaaa cggtctacct ctttgccctt agcctgccag
     2341 ccctgctggc gccgatcgtc gcggatcccg acgacggccg ggcacctgcc ctgccggtca
     2401 ccgaagaaca agtcgcggag gcggcgtgga tctgaatcgt cgtgataccg cccgccgtgc
     2461 cgaggtcgtg cgaagcgtcc tcgaggaaat cgtgcacagc ggggtgccgg tcatcaccat
     2521 cgaggggctc cgggaagcgc tgcacgtctc gccggaagcc gcaacccgga tcgtcgagcg
     2581 gctcgtcagc gccggattgc tggtcgaggt gcgcagcggg gtctggacgc gggtcaccac
     2641 ttagccgata cataggccgt ggggctgctc gtcctctcgc ttgtactcct gcttacgatt
     2701 gcgctcggaa tcgccctggc gaagggcgtg ctgaacctga tcctgcatct gctgactgaa
     2761 cagcgagttc cggccgtgga tgcctttcgc gtcgccgtgt tcgttggcgc gttgatcgtc
     2821 ttctggtcgc tcgcgccggc catcgccgag agcccggcgg cgtccggctt gaacgcgttc
     2881 atgcgctgaa gcaaggcccc agggcccagg cctctggcct cagattcctg aggcctgctc
     2941 cgcgtcgaag tccagattgg cggtcacgcg tttcccgtca gccttgaacg ccgctcgcag
     3001 cagctcgatg cggcgcttgc cctcagccac atcgtggacg cccaggttga tgttcagcag
     3061 gtcgatatac cagggcagct tcacctcgtc ggtcacgtag gcttcgacga tttccctggc
     3121 aatcggcagc gtcgtctctt tcgagttgtc gtggacgtcg cgattgccgg cgcggcgcag
     3181 ctttgcgtac tcctcggaga gcaatcgagc gaagatcggg gcgctgaata cgtctccgga
     3241 cttcagcccc gtctccgcgt cgtcggcggt gagacgcgcc cccttgtgca gccactccca
     3301 gaggatgctc aggcggatct ccccggtcgc catgtcttcc atcaggtaga gcacgtcgtc
     3361 gtttccgaag aagtctgccg gcttgagcgc cgctgcctgc atgccctgac cgaatgcatt
     3421 gccgtactgc agcgccacgc tgagcaggtc gcgcgctcct cgaatcgtcc gcggcgcggg
     3481 ctcgagcagg agcaggccgt cggcatcctc ctgtgcgtag gtcaaccgtg gaaaaggacg
     3541 gccgagctga ttcgcctttc cggcgcgctc ccacacgggc cgcacgatgt gcaccatctt
     3601 ccagtgagcc acccacttac cactcgcgcc ttcccgctcc tcgcgctcgg cgccggccac
     3661 tgccctctcc atggcccgct tcacgccgtc gggcgaaccc acgggaatgt tgggttccat
     3721 cccgccctgc cacagcgcgt actggccgcg cgagtcgggt gtgttgaccg cacgccgcac
     3781 gcggtcctcg tagttccgca tgtagccgta ggtcatcgtg atggcgtcga tgttgggatc
     3841 gacgaacgcg ggatcccagg ccatcgcgtc agcgacgctg ttgatgtagt cccagcggcc
     3901 ggtgttgaag ccgacgaagt gagaccccaa cgccgcgcga atctccatca actggtagac
     3961 ggcttcgagc tgctcgacga ggacatacac cttgattcgc ccgtcaggca ggcccagatg
     4021 ccgctcgagg gcgccgatca tctcgcccca gagcgcggcc tcctcggcgg tctggatctt
     4081 gggtagatac agtacaaggg aggcgccggt ctcgcgcatc tggcgctcgt tgttgacgac
     4141 gtagagcgcc atgtccacga tcgacgcgga gaaccctccg ccgtccgcgc gccggacgtg
     4201 gcggtcgtcc aggtgaaggc cgcgtgccct gaagatcttc gtcgtgaagt cgagctgcgt
     4261 acgccagtcg gagatgatcg gccggccgaa gaagtccttc gcccactggt tcatctcccc
     4321 cg
//
LOCUS       DBNGSH010000817         5162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_434241, whole genome shotgun sequence.
ACCESSION   DBNGSH010000817 DBNGSH010000000
VERSION     DBNGSH010000817.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_434241"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1047)
                     /locus_tag="AC16CY_18780"
     CDS             complement(34..1047)
                     /locus_tag="AC16CY_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355904.1"
                     /translation="MAASAQWFNYPTPGTPRLPDGKPDLSAPAPRTPDGKPDLSGVWS
                     GAGPMYRFNITQDLQPQDIQPWAEELFLQRVRESRKDSPLAKCMPVSLPYHQFFNLTR
                     IIQTPTLMVIMHESPNSPYRMVYTDGRDHPKDPNPTYMGYSIGRWDGDTLVVTTTGFN
                     DKGWLDSAGHPQTESLRVTERFRRRDFGTMEFDITIDDPKAFNKPFTIRTQRRLQPDT
                     DLLEDVCENEKSRAHFSVDTPIRLSPAIVAAYAGVYELAPGRDVTITVTGDLVYVAGL
                     NEPKLPLLVQSETKFMSTATPAGFEFVKDGQGRVTHLIVRGSGGQGEQKAARKADSAP
                     ARK"
     gene            complement(1134..3068)
                     /locus_tag="AC16CY_18785"
     CDS             complement(1134..3068)
                     /locus_tag="AC16CY_18785"
                     /inference="COORDINATES: protein motif:HMM:NF040438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HIR3355905.1"
                     /translation="MRRICAKVASCLGRICCLLLVAHAASAEVVRIAITRKDDAGTHE
                     RVIARVHYAIDPVLPANRAIADLDRAPTNNQGKVEFSGDLLMFVPKDAASARGSVFLE
                     VVNRGRDQSLGLMSGAAQRDLSPESWVLGDHFLLREGFTVAFLGWQFDVGRSQGLTFQ
                     VPIAQVEGVVRASYVESVRGRRRTDFPVSYCSPAQDQKTAALTFRTRIDAAPRTLSRD
                     AWQFAADGCSVHVPDGLEVGLYEVVYGAKGSPIAGLGLAAVRDFASYLRHGPSGAALR
                     ERPAAVQRVIGFGYSQSGRFLREFVRDGFNADERGRAAFDALMVSAAGAGGGSFNHRF
                     AMPGQAGNSVLSVLRPVDLPPFTDEGLLARSRAAGVTPKIFYTFSSTEYWARAGSLTH
                     TTEDGKSDVPLAATSRLYFLAGTPHASGALRPMTGQGQAGEVHANFAQQRWVLRALLL
                     ALDDWTARGIEPPASQYPSIGRGELVPRSNVRFPAMTQLQLPGYMPQVWRMDFGEGYQ
                     ATRVITNEPPRLGAAFPVLVPQVNDDGNDVAGIRMPEVAVPLGTYTAWLVPYPELSGL
                     GYLSGLSGAFVPFAATRGGRQQGDTRLSIDERYRGKQDYLDRVKRSAEDLVRARFMLA
                     RDTGAVLERAEAVWNAVVGN"
     gene            complement(3154..3978)
                     /locus_tag="AC16CY_18790"
     CDS             complement(3154..3978)
                     /locus_tag="AC16CY_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019029785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355906.1"
                     /translation="MISRRFVPAGLIAGCILALTSVATVSANHAWNVYHWARTTTSFD
                     LTVINSTTVDWDPFVTTALGDWSTSAVLNMVEDTSGSTDATVRRRCKAPSGMVRICNL
                     AYGFNGWLGIAGISIDPAGHIISGYTKLNDSYFSQSYYDRDDWRQSVTCQELGHNVGL
                     GHQDENFNNTSLLSCMDYQDPPFPFANEHDFDQLEIIYGHLDSYNSYGTGSGGGGGGG
                     CNAPPGQGCNKLDVGDGNSRREWGLSLGRRGARESFIRIDPDGTRHITFVTWARGN"
     gene            complement(4123..4623)
                     /locus_tag="AC16CY_18795"
     CDS             complement(4123..4623)
                     /locus_tag="AC16CY_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355907.1"
                     /translation="MKHALTCTIRLAVALACMAAPACENNSGPSSVETAAQVQGTWTG
                     EYTVSACNDQSVPGFCGSFVPVGTVLPIQFVLTQSGQSLSGTVELGAFSVPVSGTING
                     SRIVLSGSGTVPIEGFSTTVTLSNWNTAVSGLNMTGTWRTTFSIAGFPGSAFIDSTIR
                     VVTKTA"
     gene            4770..>5162
                     /locus_tag="AC16CY_18800"
     CDS             4770..>5162
                     /locus_tag="AC16CY_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355908.1"
                     /translation="MVPSLAWFVAAPFAQGSKLRPPVFQLDPKWPTIPNNWVLGEVTS
                     ISVDRNDNIWVLHVPQSIPEDKRANAAPPVLQFDGAGKLLTSWGGPGNGAEWLGREHG
                     IFVDANDFVWIGGRAGWPRATTPGNSDDM"
BASE COUNT          900 a         1680 c         1688 g          894 t
ORIGIN      
        1 ctgtccgagt tcttcgccgc cgcgcggtaa ccgctacttg cgcgccgggg cggaatcggc
       61 cttgcgggcg gccttctgct ctccctgtcc cccggagccg cggacgatca ggtgcgtgac
      121 cctgccctgg ccgtccttga cgaactcgaa gccggccggc gtcgccgtcg acatgaactt
      181 cgtctccgac tgcacgagca gcggaagctt cggctcgttc agcccggcga cgtacaccag
      241 gtcgccggtc accgtgatcg tgacgtcgcg accgggggcc agctcgtaca cgccggcata
      301 ggccgcgacg atcgccggac tcagcctgat cggcgtgtcc actgaaaaat gagcccggga
      361 cttttcgttc tcgcagacgt cttcgaggag atctgtgtcc ggctgcagac gccgctgcgt
      421 tcgtatggtg aacggcttgt tgaacgcctt cgggtcgtcg atggtgatgt cgaactccat
      481 ggtgccgaag tcgcgccgcc ggaagcgctc cgtcacgcgc agcgactcgg tctgcggatg
      541 gccggcgctg tcgagccacc ctttgtcgtt gaaaccggtc gtcgtcacga ccagcgtgtc
      601 cccgtcccat cgcccgatcg agtagcccat gtaggtcggg ttcggatcct tgggatgatc
      661 gcgtccgtcg gtgtagacca tgcgatacgg gctgttcgga gactcgtgca tgatgaccat
      721 cagcgtgggc gtctggatga tcctcgtcag gttgaagaac tggtggtacg gaaggctcac
      781 gggcatgcac ttggcgagcg gactgtcttt cctcgactcc cgcacgcgct gcaggaagag
      841 ctcctcggcc cagggctgaa tgtcttgcgg ctggagatcc tgcgtgatgt tgaagcggta
      901 catcgggccg gcgccgctcc acacaccgga gagatccggc ttgccgtccg gggtccgagg
      961 cgccggcgcg gacaagtcgg gcttgccatc gggcaggcgc ggcgtccccg gcgtcgggta
     1021 gttgaaccac tgggccgaag cggccaccgg cacgagtgcg gccagaacca cggtgaacgc
     1081 acagctgaac ctgactggtt ttgagttttg cgttttgcgt tttgagttac acgctagttc
     1141 ccgaccaccg cgttccatac agcctccgct ctctcgagca ccgctcccgt gtccctggcg
     1201 agcatgaagc gcgctcgcac gagatcctcc gccgatcgct tcacccgatc gagatagtcc
     1261 tgctttccgc ggtagcgctc gtcgatcgag agtcgcgtgt cgccttgctg ccggccgccg
     1321 cgcgtcgccg caaacggcac gaacgccccg ctgaggccgc tgaggtaccc caggccggag
     1381 agttccgggt acggcacgag ccatgcggtg taggtgccga gcggcaccgc cacttcgggc
     1441 atgcggattc cagccacgtc gttaccgtcg tcgttcacct gagggaccag caccgggaac
     1501 gccgcaccca gccgcggcgg ctcgttcgtg atgaccctcg tcgcctgata gccttcgccg
     1561 aaatccatcc gccacacctg cggcatgtat ccggggagct gaagctgcgt catggccggg
     1621 aagcgaacgt tcgatcgcgg tacgagctct cccctgccga tcgaaggata ctgcgacgcg
     1681 ggcggctcga tgcccctggc tgtccagtcg tcgagagcga gcagcaacgc gcgcaggacc
     1741 catcgctgct gcgcgaagtt ggcgtgcact tctcccgcct ggccctggcc cgtcatcggc
     1801 cgcagcgcgc cggaggcgtg cggcgtcccg gccaggaagt acaggcgcga ggtggccgca
     1861 agcggcacat cgctcttgcc gtcctcggtc gtgtgcgtga gcgacccggc tcgcgcccag
     1921 tattcggtcg acgaaaacgt gtagaagatc ttcggcgtca cgcccgccgc ccgtgaccgc
     1981 gcgagcagcc cctcgtccgt gaacggcgga agatcgaccg gccgcagcac ggacagcacc
     2041 gagttgcccg cctgacccgg catcgcgaac cggtggttga agctgccgcc tccggcgccg
     2101 gccgccgaca ccatcagcgc gtcgaacgcg gcacgtccgc gctcgtcggc gttgaagccg
     2161 tcgcgaacga actcgcgcag gaagcgcccg ctctgcgagt agccgaagcc gatgacccgc
     2221 tgcaccgccg cgggccgttc gcgcagagcg gcgcccgagg gcccgtggcg gagatacgag
     2281 gcgaaatcgc gtaccgcggc gaggccaagg cccgcaatcg gcgatccctt cgccccgtag
     2341 accacctcgt acaggccgac ctccaatcca tcgggaacat gcaccgagca cccgtcggcg
     2401 gcgaactgcc atgcgtcgcg agacagcgtt cgcggcgcgg cgtcgatgcg tgtacgaaac
     2461 gtcaacgccg cggtcttctg atcctgagcc ggcgaacagt acgacacggg aaaatcggtg
     2521 cggcgtctcc cccggacgga ttcgacgtaa ctggcgcgca cgacgccttc gacctgcgcg
     2581 atcggcacct ggaaggtcag gccctgcgaa cggccgacgt cgaactgcca gccgaggaac
     2641 gccaccgtga atccctcgcg cagcagaaag tggtcgccca gcacccacga ctccggggag
     2701 aggtcccgct gagcggcgcc gctcatcagg ccgagcgatt gatcgcggcc gcgattgacg
     2761 acttcgagga acaccgaccc tctggcggac gcggcatcct tgggaacgaa catcaggagg
     2821 tcgccggaga actcgacctt cccctggttg ttcgtcggag cacggtcgag atcggcgatg
     2881 gcgcgattgg ccgggagcac cgggtcgatg gcgtagtgca cgcgcgcgat cacccgctcg
     2941 tgcgtgcccg catcgtcttt ccgcgtgatc gcgattcgga cgacttcagc ggatgcggcg
     3001 tgcgcgacga gcagcaggca acagatccgg cccaggcagg aagcgacctt cgcacagatg
     3061 cgtcgcatcg atgcgaagta taggaccgaa aatggggtcg ggagtcattt ttgaggtgcc
     3121 ctcaagaaaa atgactcccg acccccttga ggtctagtta cctcgagccc aggtcacgaa
     3181 cgtgatgtgg cgggtgccgt cgggatcgat gcggatgaag gactcgcgcg cgccgcgtcg
     3241 gccgagcgag aggccccatt cgcgccggct gttgccatcg ccgacgtcga gcttgttgca
     3301 gccctggcct ggaggggcgt tgcacccacc gccgcccccg ccgccgcttc ccgtgccata
     3361 ggaattgtag gagtcgaggt gtccgtagat gatctcgagc tggtcgaagt cgtgctcgtt
     3421 ggcgaacggg aagggaggat cctggtaatc catgcacgag agcagcgacg tgttgttgaa
     3481 gttctcgtcc tgatgaccga ggccgacgtt gtgccccagc tcctgacagg tgacgctctg
     3541 tctccagtcg tcgcggtcgt agtagctctg gctgaaatac gagtcgttca gcttcgtgta
     3601 tccggagatg atgtggcccg cgggatcgat cgagatgccg gcaataccca gccacccgtt
     3661 gaatccgtac gccaggttgc agatgcggac catccccgag ggagctttac accgccggcg
     3721 aacggtcgcg tcggtcgaac cgctcgtgtc ctcgaccatg ttgagcacag cggacgtcga
     3781 ccagtcgcca agcgccgtcg tcacgaacgg gtcccagtcg acggtcgtgc tgttgatgac
     3841 cgtcagatcg aaggatgtcg tcgtccgtgc ccagtgatag acgttccagg cgtgattcgc
     3901 tgaaaccgtg gcaacggagg ttagcgccag gatgcagccc gcaatgagtc cggccgggac
     3961 gaagcgtcgt gaaatcatga tgagggagct aacgcacgat tcgggccagt tcgaaatcgg
     4021 gaagaactcg cgaaggtttg ggaaccgtgc gaccggagtc tgccaaagcg tgacatttct
     4081 gatcggcttc gccagaaaac ggcgttccga gccgaccctc gcctacgcgg tttttgtcac
     4141 gactcgaatg gtgctgtcga tgaaggccga cccagggaac ccggcaatgc taaaggtcgt
     4201 ccgccacgtg ccggtcatgt tcaagcctga cacggccgta ttccagttcg agagcgtgac
     4261 tgtggtcgaa aaaccctcga tgggtacggt cccactgccg ctcaggacga tgcggctgcc
     4321 gttaatggtg ccggagacgg ggaccgagaa agccccgagt tcgacggttc cggagagcga
     4381 ctgtcccgac tgggtcagga cgaactgtat cggcagcacc gtacccacgg gcacgaagct
     4441 accgcagaac ccgggaacag actggtcgtt gcacgccgac acggtgtatt cgccggtcca
     4501 ggtcccctgg acctgggccg ctgtctcaac cgaagacggg ccggagttgt tttcacacgc
     4561 gggggcagcc atgcaggcaa gtgcaacggc cagccggatc gtacaagtca gcgcatgctt
     4621 catctgcgtg tgtctccact gctgagccac attaggcgcc gcgctacaat gccggatcct
     4681 ggggtctgac cccgacagat actctccgga gcgcgaacat gtccgtactc actcgacgat
     4741 ctttttcggc gctggccact gcagccgtta tggttcccag ccttgcctgg ttcgtggcgg
     4801 ctccctttgc ccagggcagt aagcttcgcc cgcccgtgtt ccagctcgat ccgaaatggc
     4861 cgaccattcc caacaactgg gtcctcggcg aggtgacgtc gatctcagtc gaccgtaacg
     4921 acaacatctg ggtgctgcac gtgcctcagt cgattcccga ggacaagcgc gcgaacgcgg
     4981 cgccgccggt gcttcagttc gatggtgcgg gaaagctgct gaccagctgg ggcggacccg
     5041 gcaacggcgc cgagtggctc gggcgcgagc acggcatctt cgtcgatgcg aacgacttcg
     5101 tctggatcgg cgggcgcgcc gggtggccgc gcgccacgac gcccggcaac agcgacgaca
     5161 tg
//
LOCUS       DBNGSH010000818         3653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2302944, whole genome shotgun sequence.
ACCESSION   DBNGSH010000818 DBNGSH010000000
VERSION     DBNGSH010000818.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2302944"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..494)
                     /locus_tag="AC16CY_18805"
     CDS             complement(123..494)
                     /locus_tag="AC16CY_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3355909.1"
                     /translation="MTPDDLKARTKKFAVDVIRFAKAIPTDPINNEIAVQLTDAATST
                     AAHYRAVCRARSRADFINKLSGAVEEVDESALWLEILAESGISQASVTTPLWREADEL
                     TRILVSSRETAKANRQSFQTR"
     gene            complement(646..2550)
                     /locus_tag="AC16CY_18810"
     CDS             complement(646..2550)
                     /locus_tag="AC16CY_18810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit B"
                     /protein_id="HIR3355910.1"
                     /translation="MSTYTAKDITVLEGLEPVRKRPGMYIGGVGSAGLHHLVWEILDN
                     AVDEAMNGFASNIVVTLHKDASSITVSDDGRGIPVDKHTSTKKSALEVIFTMLHAGGK
                     FEKGSYKTAGGLHGVGASVVNALSTELRATVKRDGAQWEMAFRRGKPTGALKKVGAAR
                     GSGTTVYFHPDPTIFPKIEFDAAVVRERLEVVSYLHKGVRVVFENEAEGGKDVFQHQE
                     GISDYLKNIVASRGAKAVHDAPFLLERESGPRLDLVLQWTEATDEHVRSYVNGIPTGS
                     GGTHDNGLRSGLGKAVRNYIETHSLSPKGVTLTAEDIREGLTGVLSLFIEEPQFQGQT
                     KDRLNNPEMSSAIDSAVRPALEHWLNHNRSAAEAIVGRIILAARAREASRAAQAEVTR
                     KTATSGRLNLPGKLSDCTSSNRDDTELFIVEGDSAGGSAKQGRDRAKQAILPLRGKVM
                     NTEGMTLAKVLENKELADLVTALGCGMGKGFDLSRLRYGRVIILADADSDGHHIATLL
                     LTFIYRHLPQLISGGKVFLAQPPLYRIDIGKDTYWALDDAHRDRLLKQHGNGRGSPEI
                     TRFKGLGEMMPKVLWETTLSPKTRRLLRVEIADQLVTDRVINELMGKDASARFRFIME
                     RAEEAQELDL"
     gene            2620..>3653
                     /locus_tag="AC16CY_18815"
     CDS             2620..>3653
                     /locus_tag="AC16CY_18815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3355911.1"
                     /translation="MVRTLVALAALLLVAPPLAAQSLNPPPDAKARIDRHFERFTGRP
                     GCAVGAAINGTTVFSAGYGVADLEHAVPITADTVFEPGSVSKQFTAAAVLLLAQQGRI
                     SLDDPVRKYIPEVPDYGTPITIRHLIHHTSGLRDWGGVAAIGGWPRGTVARNHDDVLD
                     IVSRQHALNYAPGAEYSYTNTGYNLAAILVGRVTGKSFAEFTREAIFQPLGMSSSSWR
                     DDFRRVVPKRAIAYSPDGTSFALNMPFENVHGNGGMLTTVGDLLRWNTNFTDAGVGGR
                     ALVDSQLRKGVLNDGRTIDYAGGLVVGNWRGVREISHSGATAGYGGWLARYPDQGLSV
                     AILCNVSSANP"
BASE COUNT          613 a         1207 c         1142 g          691 t
ORIGIN      
        1 tgcgttcccg cgcgacggct cgagctctaa tccccaatca cccaaatccc aaatagatcc
       61 ttcaatcgac aacctccaat caacaacgaa tcaaccatca aagatctgaa aatcaccaat
      121 ggtcaccgcg tctggaacga ttggcgattc gccttggctg tttcgcggga gctgaccagg
      181 attcgagtca gttcgtccgc ttctcgccat agaggtgtcg tcacagatgc ctggctgatg
      241 cccgactcgg ccaggatttc cagccacaac gccgattcgt cgacctcctc gactgctcca
      301 ctcaacttgt tgatgaaatc ggcccgtgat cgcgcacgac agaccgcgcg atagtgcgcc
      361 gcggtcgatg tggccgcgtc ggtcaattgc acggcgattt cgttgttgat gggatctgtt
      421 ggaatcgcct tcgcgaatcg gattacgtcg actgcaaact tcttcgtccg cgctttcaga
      481 tcgtctggcg tcatgcccgt gaagcgagca cgccgcgtgc caatcaacaa tcaggcgcga
      541 ggcagcctca tcgtccaata aatccccaat caataatcaa aaatcacaaa cgaatcgaca
      601 atcaaagatc gcaaaatctt caattgaagg attgggcagt cgagatcaga gatcgagctc
      661 ctgcgcttct tcggcgcgct ccatgatgaa gcggaagcgg gcggaggcgt ccttccccat
      721 cagctcgttg atcacgcgat cggtgacgag ctgatcggcg atctcgacac gcagcagccg
      781 ccgcgttttc gggctgagcg tcgtttccca cagcaccttc ggcatcatct cgccgagtcc
      841 cttgaagcgg gtgatctcgg ggctgccgcg gccgttgcca tgctgcttca gcagccgatc
      901 gcggtgggca tcgtcgagcg cccagtaggt gtccttgcca atgtcgattc gatagagcgg
      961 cggctgcgcg aggaacacct tcccgcccga gatgagctgc ggaaggtggc ggtagatgaa
     1021 cgtgaggagc agcgtggcga tgtggtggcc gtccgagtcg gcgtcggcca ggatgatgac
     1081 ccgcccgtag cgcagccggc tcaagtcgaa gcccttcccc attccgcatc cgagcgccgt
     1141 gacgagatcg gcgagctcct tgttctcgag caccttggcg agcgtcatcc cctcggtgtt
     1201 catcaccttg ccgcgcagcg gcaggatggc ttgctttgcg cggtcgcgcc cctgcttggc
     1261 cgaccctccg gccgaatcgc cctcgacgat gaagagctcc gtgtcgtcgc ggttcgagct
     1321 cgtgcagtcg ctgagtttgc ccgggaggtt caggcggccg gacgtcgccg tcttgcgcgt
     1381 cacctccgcc tgcgccgccc tgctcgcttc gcgcgcccgc gcggcaagga tgatgcggcc
     1441 gacgatcgcc tccgccgcgc tccggttgtg attgagccag tgctcgagcg cggggcggac
     1501 cgcagagtcg atcgccgacg acatctccgg gttgttcagc cgatccttgg tctgcccctg
     1561 gaactgcggc tcctcgatga agaggctgag cacgccggtc agcccctcgc ggatgtcttc
     1621 ggccgtcagc gtcacaccct tgggcgacag gctgtgcgtc tcgatgtagt tgcgaacggc
     1681 cttgcccagg cccgacctca gcccgttgtc gtgcgtgccg cccgagccgg tcgggatgcc
     1741 gttgacgtag ctgcgcacgt gctcgtcagt cgcctcggtc cactgcagca cgaggtccag
     1801 ccgcggcccg ctctcgcgct cgaggagaaa cggggcgtcg tgcaccgcct tggcgcctcg
     1861 agaggcgacg atgttcttga ggtagtcgga gatcccttcc tggtgctgga agacgtcctt
     1921 gcctccctcc gcttcgttct cgaagacgac gcgcacgccc ttgtgcaggt agctgacgac
     1981 ctcgagccgc tcccggacga cggcggcgtc gaattcgatc ttcggaaaga tcgtcggatc
     2041 ggggtgaaag tacacggtcg tgcccgagcc ccgcgcggcg cccaccttct tgagcgcgcc
     2101 ggtcggcttg ccgcggcgga aggccatctc ccactgcgcc ccgtcgcgct tgaccgtcgc
     2161 ccgcagctcc gttgaaagcg cgttgacgac gctggcgccg acgccgtgca gcccccccgc
     2221 ggtcttgtag ctacccttct cgaacttgcc cccggcgtgg agcatcgtga agatcacttc
     2281 gagcgcgctc ttcttcgtgg acgtgtgctt gtcaaccgga atgccccgtc catcgtctga
     2341 gacggtgatg gagctggcgt ccttgtgcag ggtgacgaca atgttcgagg cgaagccgtt
     2401 catcgcctcg tctacggcgt tgtcgaggat ttcccacacc aggtggtgca gcccggcgct
     2461 gccgacgccg ccgatgtaca tgccggggcg tttgcggaca ggctcgaggc cctcgaggac
     2521 ggtgatgtct ttggcggtgt aggtggacat gaggctatcg gctgcgagcc ccggcccgtc
     2581 taaggtagca tgcggattcc tggtcgagag gggatgaaca tggtacgcac gcttgttgcg
     2641 cttgctgcgc ttctcctggt cgcgcctcct ctggccgcgc agagcctcaa cccgccgcca
     2701 gacgccaagg ccaggatcga tcgccacttc gagcgcttca ccggccgccc cggctgcgcc
     2761 gtcggcgcag cgataaacgg gacgacagtc ttctcggccg gatatggcgt agccgacctg
     2821 gaacacgctg ttccgatcac ggctgatacg gtcttcgaac ccggctcggt gagcaagcag
     2881 ttcaccgcgg cggcggtgct gctgctggcg caacagggac gcatctcgct cgacgacccg
     2941 gtgcgcaagt acattccgga agtccccgac tacggaacgc cgattaccat ccggcatctc
     3001 atccaccaca ccagcggact gcgcgactgg ggcggcgtgg cggccatcgg gggctggccg
     3061 cgaggcaccg tggcacgcaa tcacgacgac gtgctcgaca tcgtgagccg tcagcacgcg
     3121 ttgaactacg cgcccggcgc cgaatactcg tacacgaaca ccggctacaa cctggccgcg
     3181 atcctggttg gccgcgtcac cgggaagtcg ttcgccgagt tcacgcgcga ggcgatcttc
     3241 cagccgctcg gcatgtcgtc gtcgtcgtgg cgcgacgatt tccgccgcgt cgtgcccaaa
     3301 cgcgcgatcg cgtactcgcc cgacgggacg tccttcgcct tgaacatgcc gttcgagaac
     3361 gtccacggca atggcgggat gctgaccacg gtcggcgacc tgctgcgatg gaacaccaac
     3421 ttcacggacg ccggcgttgg cggccgcgcg ctggtggaca gccagcttcg caagggcgtg
     3481 ctgaacgacg gccgcaccat cgactacgcc ggaggcctgg tcgtcggaaa ctggcgcggt
     3541 gtgcgcgaaa tcagccacag cggcgctacg gccggttatg gaggctggct tgcgcgttat
     3601 cccgatcagg gtctgtcggt ggcgattctc tgcaacgtca gcagcgccaa ccc
//
LOCUS       DBNGSH010000819         7174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4000252, whole genome shotgun sequence.
ACCESSION   DBNGSH010000819 DBNGSH010000000
VERSION     DBNGSH010000819.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4000252"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..3117)
                     /locus_tag="AC16CY_18820"
     CDS             complement(1..3117)
                     /locus_tag="AC16CY_18820"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3355912.1"
                     /translation="MGVPMAALAQATLTGVVRDTSGAVLPGVTVEATSPVLIEKVRTG
                     VTDGTGRYQIIDLRPGLYTVTFSLESFSVVKREGVELTGTLTTTANAELRVGTLQETV
                     TVTGETPTVDVQSSTRIAVLDKEVIDTLPTSRNSFSLGVLIPGMNVRNGFGPVTDVGG
                     ATGPSTLALNIHGGKTEDQRLLVNGVALSTMIGGGWGGGAIPNATGLSEIAYDYSGVD
                     ATSATGGVRINFIPRDGGNRVTGTIMGSFAADSMQPDTFTTVTRGNTTFDFPDPNNPG
                     QRIGFRASTVKGNGEFNPGVGGPLVQDRLWYFVSGRYQAANSWVTGAFHNANAGNPNA
                     WTLVKDFERPAYNPRAWNVYMGRLTWQAAPKHKIGFTYDHEWFCECAISTSALNSPEI
                     STEFNFPLQRFIQLDWNSPVSNRILLEASGIHRVERWGGMHMTHEKAGETAPPPGMVG
                     VNEQTTGENYRANPGLFPFFAAPFNNSWNVNLHYRAALSYITGSHQLKVGFNNAWGHH
                     ENTAYHDSSPYSYMFSGGRPSQIRQWASPYTTQVDVDADLGIYAQDRWTLGRATISLG
                     VRYDHFVNSYPPQTLGPGPLVPNRNVTYDNGTYDASTGLLRPGAQPNGEPIPNVSWND
                     ITPKLGLTYDLFGNGKTALKASLNKYLAGMGTFSFATFNSVTSANNPINRLSNNATRQ
                     WLDDGSGGGIAGDFIPQCVLTNVNANGECGVLSNTNFGAVLAPTTSYDPDYLTGWGKR
                     YYNWEFSVGVQHEIAPRVSTEVSYFRRSYGNFPVTDNRAYSSVDFASTSIVAPTDPRM
                     PDGGGQTLNGVYYTTGAPRADNFLVTFASNYGEQIEQWNGMDFSVNARLQNGVTLQGG
                     VSTGRTVTDNCDLVRHPELTELNVAGGGFGPLFFFAGTPQDFCHRDEGFITQFKGFAT
                     YTIPRIDLQVAGTYQGLPGLLVAGNYNALVGIPFKVVQVVEPGSLYGDRMNQFDFRLS
                     KLLRFGGTRTMVGLDIYNLLNSTPVLSENAAMGFDPAPGQPAYPAWQSPLTMLQSRFF
                     KVSAQFDW"
     gene            3285..4220
                     /locus_tag="AC16CY_18825"
     CDS             3285..4220
                     /locus_tag="AC16CY_18825"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3355913.1"
                     /translation="MDLRRVKAFIAVAETSSVSKAAERLHISQPPLSRQIHLLEEELG
                     VTLFVRHHHGVTLTDAGRRLLDKARAVDAAAADFCGAAQQVTCAEASEIRIGIGWGLW
                     DIVNSIRVDFAKQYPDVTITATDAFCWYDSDDQLRNRTLDIAFARPPYDEGFDVSQPL
                     FQERIQAIVSDVSPLAAARTVSVRDLANETLLLWDRHVAPVLYDRILELYARAGIEPR
                     TLSTPGAGPFNHAGMMLVASGKGVYLGYGVPLTEPQPPTGVAVRPLSDPGATTEVCVV
                     RRKHDDSPVVKAFLESVWRVFPREQYAPIAVGSRR"
     gene            4231..6030
                     /locus_tag="AC16CY_18830"
     CDS             4231..6030
                     /locus_tag="AC16CY_18830"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3355914.1"
                     /translation="MRRPTLTAAIVLACLATSVPARAQDPFHQTVVVTASARPVELGR
                     VTRTMTVITREQIDVLPLKSVADVLRLASSVDVRARGVRGVQTDFALRGANFTQMLML
                     VDGVRLNDPQSAHHNGDIPVPLDLVERIEVLYGPGSSLHGADAVGGTINIVTRRAADA
                     ALVVEGGSYELAAGRGEYGFERGSLTQAFGGSFDRSSGFMYDRDFKTAIVRSRTSVGD
                     RSGVSVSYLWKEFGANNFYGGNAPSREWTNQTLVTGDRRFGDVGGWSIDSQASYRTHG
                     DRFVFNQLNPALSDNRHRTHTVLGSLSGSRGVGGAAVTIGFESGGDWIRSTNLGDHST
                     ARVSGFGEWRQQFGTSMQVDGLLRLDWYDEFGASWNPSLGVGWWATPSVRVRASAAHG
                     FRVPTFTERYYSDPANLAREEVGPETSWAGEGGVDVLMPKGVVLQASFFGRADSDVID
                     WLRSTTAERWRTYNIRDVDTLGIELGARKTFASGAFLQGQYTALDVSAPTVTQLSKYV
                     LDYAPHSIAVAAGVPLPWQFRVAPRIEYRRRSRSTGTSDYVLLDAVVGRRFGPMFELL
                     VDGANLLDAEYQEISGVAMPGATVSISLAIGAR"
     gene            complement(6113..>7174)
                     /locus_tag="AC16CY_18835"
     CDS             complement(6113..>7174)
                     /locus_tag="AC16CY_18835"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HIR3355915.1"
                     /translation="MPAEWEPHRATWIAWPHHEPDWPGKFGPIPWVYAEIARVIARHE
                     PLEILCQSAEVRESAREVLEAHGVDRQRVRLHVVPTDRVWLRDSAPTGVHDASGHVVL
                     LNWAFTAWSKYDNWSQDSKVGQEMSALTGLRREEPCRPDGGARVVLEGGGIEVNGGGL
                     LLVTEEWLLGDVQVRNEGLDRGAYERLFAEWLGARFTIWLGEGCVGDDTHGHVDDVAR
                     FVGPETVVLAVEPDPLDGNHARSMDNLRRLELASSTAGVGPLRIVTLPFPRAVEMNGE
                     RLPASYANFYVANGVVLVPTFNDPNDRVALATLAEVLPSHEVVGIHAVDLVWGLGTIH
                     CLTQQEPQVKMGSGVIFPE"
BASE COUNT         1166 a         2315 c         2360 g         1333 t
ORIGIN      
        1 ctaccagtcg aactgcgcgc tgaccttgaa gaagcgcgac tgcagcatcg tgagcggcga
       61 ctgccacgcc gggtaggccg gttgccccgg cgccggatcg aagcccatcg cggcgttctc
      121 gctcagcacc ggcgtcgagt tgagcaggtt gtagatgtcc aggccgacca tcgtccgcgt
      181 gccgccgaac cgcaggagct tcgagaggcg gaagtcgaac tggttcatcc ggtcgccgta
      241 gagtgagccc ggctcgacaa cctgaacgac cttgaacggg atgccgacga gagcgttgta
      301 gttcccggcg accagcagcc cgggcaaccc ctggtaggtg cctgcgacct gcaggtcgat
      361 ccgggggatt gtataggttg caaagccctt gaactgggtg atgaagcctt cgtcgcggtg
      421 gcagaagtcc tgcggcgttc ccgcgaagaa gaagagtggc ccgaatccgc cgcccgccac
      481 gttcagctcg gtcagctcgg gatgccgcac cagatcgcag ttgtccgtca ccgtgcgtcc
      541 cgtgctgacg ccgccctgca gggtcacgcc gttctggagc ctggcgttga cgctgaagtc
      601 catgccgttc cactgttcga tctgttcccc gtagttgctg gcgaacgtga cgaggaagtt
      661 atctgctctg ggcgctccgg tggtgtagta gacgccgttg agcgtctgcc caccgccgtc
      721 cggcatacga ggatccgtgg gcgcgacgat cgatgtcgaa gcaaagtcga ccgacgagta
      781 ggctctgttg tcggtgaccg ggaagttgcc gtacgaccgg cggaagtagc tcacctccgt
      841 cgatacgcgg ggcgcgatct cgtgctggac gcccaccgag aactcccagt tgtagtaccg
      901 cttgccccag ccggtgaggt agtcggggtc gtaggacgtg gtcggcgcca gcaccgcgcc
      961 gaagttcgta ttcgagagga cgccgcactc gccgttggcg ttgacgttgg tgaggacgca
     1021 ctgcgggatg aagtcgcccg caattccgcc tccgcttccg tcgtccaacc attggcgcgt
     1081 cgcgttgttc gagaggcgat tgatcggatt gtttgcggac gtcacgctgt tgaaggtggc
     1141 aaaggagaac gttcccatgc cggccaggta cttgttcagg ctggccttga gcgccgtctt
     1201 gccgttgccg aacaggtcgt aggtcaggcc gagcttcggc gtgatgtcgt tccagctgac
     1261 gttcgggatg ggctcaccgt tcggttgtgc gcccggccgc agaaggcccg tcgaggcatc
     1321 gtaggtcccg ttgtcgtagg tcacgttccg gtttggaacg agcggtcccg gcccgagagt
     1381 ctgtgggggg tagctgttca cgaagtggtc gtaccggacc ccgagtgaga ttgtggcccg
     1441 gccgagcgtc cacctgtcct gggcatagat gccgaggtcg gcatctacat cgacctgcgt
     1501 cgtgtatggg cttgcccact gcctgatctg actcggcctg ccgccgctga acatgtagga
     1561 gtaggggctg gagtcatggt acgccgtgtt ctcgtgatgc ccccacgcgt tgttgaagcc
     1621 gaccttgagc tgatgggagc ccgtgatgta ggacagggcg gcccggtagt gaaggttgac
     1681 gttccacgag ttgttgaagg gagccgcgaa gaaggggaag agccccgggt tcgcgcgata
     1741 gttttcaccc gtcgtctgtt cgttcacgcc gaccattccc ggcggagggg ctgtctcgcc
     1801 ggccttttcg tgcgtcatgt gcatgccgcc ccatcgctcg acgcggtgga tgccgctcgc
     1861 ttcgagcagg atgcggttgc tgacgggcga gttccagtcg agctggatga atcgctgcag
     1921 cgggaagttg aactcggtcg aaatctccgg tgagttcaac gccgaggtcg agatggcgca
     1981 ctcgcagaac cactcgtggt cgtaggtgaa gccgattttg tgcttcggcg cagcctgcca
     2041 cgtcaggcgg cccatgtaga cgttccaggc gcgcgggttg tacgccggcc tttcgaagtc
     2101 cttcacgagc gtccaggcgt ttggattgcc cgcgttcgcg ttgtggaacg cgccggtgac
     2161 ccaggaattg gcagcctgat agcgccccga cacgaagtac cacagcctgt cctggacgag
     2221 tggaccgccg acgccgggat tgaactcgcc gtttcccttg accgtgcttg cgcggaaccc
     2281 gatccgctgc cctgggttgt tcgggtccgg gaaatcgaag gtggtgttgc ctctcgtgac
     2341 ggtcgtgaac gtatccggct gcatcgagtc ggccgcgaag ctgcccatga tcgtgcccgt
     2401 cactctgttg ccgccgtcgc gcgggatgaa attgatccgc acgccgcccg tggctgacgt
     2461 tgcgtcaacg cccgagtagt cgtacgcgat ttcggagagc ccggtcgcgt tgggaatggc
     2521 gccgccgccc catccgccgc cgatcatcgt gctgagcgcc acgccgttga ccagcaggcg
     2581 ctgatcctcg gtcttgccgc cgtgaatgtt gagggcgagc gtgctgggtc cagtcgcgcc
     2641 gccgacgtca gtcacaggac cgaacccgtt tctgacgttc attcccggaa ttagcacacc
     2701 gagtgagaag gaattgcgcg acgtcgggag ggtgtcgatg acttccttgt cgagtaccgc
     2761 gattcgggtc gagctctgca cgtccactgt cggcgtttcg cccgtgaccg tgaccgtctc
     2821 ctgcagggtg ccgaccctca actctgcatt cgccgtggtc gtgagggtgc cggtcagctc
     2881 gacgccctcc cgcttcacca ccgagaagct ctcgagcgag aacgtgacgg tgtacaggcc
     2941 cggccgcaga tcgatgatct gatacctgcc ggtgccatcg gtcacgcccg tgcggacctt
     3001 ttcgatcagc acggggctgg tcgcttcaac tgtgacgccg ggcaacacgg cgccagacgt
     3061 atctctcacc acgccggtga gtgtggcctg ggccagcgcc gccatcggca cgcccataac
     3121 cattagtgct gcaacgacaa ccctccgcaa agcacgcatg cccaaccctc cttcggccgc
     3181 aggttttgac acgtagacgg cgataccgtc gcccacctcg cgttttgtgt ccaatacatc
     3241 tttgttaccc gggtataccg acttgatata caatccgcgg ccaaatggac ctgaggcgag
     3301 tcaaggcgtt cattgcggtc gccgagacat cgagcgtgtc caaagccgca gagcggctgc
     3361 acatctcgca accccctctc agccgtcaga tccatctgct cgaagaggag cttggtgtca
     3421 ccctgttcgt ccggcaccac cacggcgtca cgctcaccga tgccgggcgg cgcctgctcg
     3481 acaaggcgcg cgccgtcgac gcggccgccg ctgatttctg cggtgcggcg cagcaagtca
     3541 cgtgcgccga agccagtgag attcgcatcg gcatcggctg gggcctctgg gacatcgtca
     3601 acagcattcg agtcgatttc gccaagcagt acccggacgt gacgattacg gcaacagacg
     3661 ccttctgctg gtacgactcg gacgaccagc ttcgaaaccg tacgctggac atcgcgttcg
     3721 cgcgtccacc gtacgacgag ggtttcgacg tctctcagcc tctgttccag gagcgcatcc
     3781 aggcgatcgt ctccgacgtg agccctctcg ccgcggcccg gacagtcagc gtcagggatc
     3841 tcgccaacga gacgctgctg ctgtgggacc ggcacgttgc gccggtcctc tacgaccgca
     3901 tcctcgagct gtacgcgcgc gcaggcatcg agccccggac gttgtccacg ccgggagccg
     3961 gaccgttcaa ccacgcgggc atgatgctcg tagcctccgg caagggcgtg tacctcggct
     4021 acggagtacc tctgaccgag ccgcagcctc ccaccggagt agctgtcagg cccctgagcg
     4081 accctggggc gacgacggag gtgtgcgtcg tccgccgcaa gcacgacgac tcgccggtgg
     4141 tgaaagcgtt tctcgaaagc gtctggcgag tatttccccg agagcaatac gcacccatcg
     4201 ccgtcggctc gaggcggtag gtcagcgcgg gtgagacgac cgacgctcac cgcggccatc
     4261 gtcctcgcgt gtctcgccac gtccgttcca gcccgcgccc aggatccatt tcatcagacc
     4321 gtcgtcgtga cggcctcggc caggccggtg gagcttggcc gcgtgacgcg gacgatgacg
     4381 gtgattacgc gggagcagat cgacgtgctc ccgctgaagt cggtggccga tgtgctccgg
     4441 cttgcctcgt cggttgacgt tcgtgcgcgc ggcgtgcgcg gcgttcagac cgacttcgcc
     4501 cttcgcggcg ccaacttcac gcagatgctc atgctggtgg acggcgtgcg cctgaacgac
     4561 ccgcaatcgg cgcatcacaa cggcgacatc cccgttccgc tcgatctggt cgagcgcatc
     4621 gaggtgctct acggccccgg gtcgtcgctg cacggtgcgg acgccgtcgg cggcacgatc
     4681 aacatcgtca cgcggcgcgc cgctgacgcg gcgctcgtcg tcgaaggggg cagctacgag
     4741 ctggcggccg gtcgaggcga gtacggtttc gagcgcggct cgttgacgca ggcgttcggc
     4801 ggctccttcg atcgatcctc cggcttcatg tacgaccggg acttcaagac cgcgatcgtt
     4861 cgctcgcgca cgtcggttgg cgaccgttcg ggtgtgtcgg tgtcgtactt gtggaaggag
     4921 ttcggcgcga acaacttcta cggcggcaac gccccgtcgc gcgagtggac gaaccagacg
     4981 ctcgtgacgg gtgatcgccg gttcggcgac gtcggcggct ggagcatcga ctcgcaggca
     5041 tcgtaccgga cgcacgggga ccggttcgtc ttcaaccagc tgaaccccgc gctctcggac
     5101 aatcgtcatc gcacacacac cgtgctcggc tcgctgagcg gctcgcgcgg cgtcggcggc
     5161 gccgccgtca cgatcgggtt cgagagcgga ggcgactgga ttcgttcgac gaaccttggc
     5221 gatcactcga cggcgcgcgt gagcgggttc ggcgaatggc gacagcagtt cgggaccagc
     5281 atgcaggttg acgggctgct tcgcctcgat tggtacgacg agttcggcgc ctcgtggaac
     5341 ccgtcgctcg gcgtgggatg gtgggcgacg ccgagcgttc gcgtccgcgc gtcggccgcg
     5401 catgggttcc gcgtgcctac cttcacggag cggtactatt ccgatcctgc gaacctcgcg
     5461 cgggaggaag tcggcccgga aacgtcctgg gcgggggagg gcggcgtgga cgtgctgatg
     5521 ccgaaaggcg tcgtgcttca ggcgtccttc ttcggccggg ccgacagcga cgtcatcgac
     5581 tggctgcgtt cgacgaccgc cgaaaggtgg cgcacctaca acattcgcga cgtggacacg
     5641 ctggggatcg agctcggggc ccgcaagacg ttcgcgtcgg gtgccttcct gcaggggcag
     5701 tacaccgcgc tcgacgtctc ggcgcccacg gtgacccagt tgtcgaaata cgttctggac
     5761 tacgcccccc actcgatcgc cgtcgcggcc ggcgttccgc ttccgtggca gttccgcgtt
     5821 gcgccgcgaa tcgagtaccg ccgtcgatcg cgctcgacgg ggacatccga ctatgtgctg
     5881 ctcgatgccg tcgtgggccg caggttcggc ccgatgttcg agctgttggt cgatggcgcc
     5941 aacctgctcg acgcggaata ccaggagatc tcaggcgtcg cgatgccggg cgccaccgtc
     6001 agcatctcgc tcgcgatcgg cgctcggtga aaatggggtc gggagtgaat aaaagggggt
     6061 cgggagtcat tttccccgga gagtgcggtg ggaaaatgac tcccgacccc ctttattccg
     6121 ggaaaatgac tcccgacccc attttcactt gcggttcctg ctgcgtcagg cagtgaatcg
     6181 tccccagtcc ccacacgaga tcgaccgcgt gaatcccgac gacctcgtgc gaaggcagca
     6241 cctcggccag cgttgccagc gccactcgat cgttgggatc gttgaacgtc ggcacgagga
     6301 ccactccgtt ggcgacatag aagttggcgt agctggccgg caagcgctcg ccgttcatct
     6361 ccaccgcgcg cggaaacggg agcgtcacga ttcgaagcgg tccgacgccg gcagttgacg
     6421 atgccagttc gaggcgcctg aggttgtcca tcgatctcgc atggttgccg tcaagcggat
     6481 ccggttcgac ggcgaggacg acggtctctg gtccgacgaa tcgcgccacg tcgtccacgt
     6541 ggccatgcgt gtcgtcgccc acgcaccctt ccccgagcca gatggtgaag cgggcaccaa
     6601 gccattcggc gaagagccgt tcgtacgcgc cgcgatcgag accctcgttg cgaacctgca
     6661 cgtcgccgag cagccactcc tcggtcacga gcagaaggcc cccgccgttg acctcgattc
     6721 cgccgccttc cagcacaacg cgggccccgc cgtcgggacg gcacggctcc tcgcgccgca
     6781 gcccggtcaa cgccgacatt tcctgtccga ccttcgagtc ctgcgaccag ttgtcgtact
     6841 tggaccaggc ggtgaatgcc caattgagca ggacgacgtg tcccgacgca tcgtgcacac
     6901 cggtcggcgc ggaatctcgc agccagacac gatccgtggg gaccacgtgc aggcgcaccc
     6961 gttggcgatc gacgccgtgc gcctcgagca cctcacgcgc cgactcacga acctcggcag
     7021 actgacagag gatctcgagt ggttcgtgcc gcgcgattac gcgcgcgatt tcggcgtaca
     7081 cccacggaat gggtccgaac ttgccgggcc agtcgggctc atggtgcggc caggcgatcc
     7141 acgtggcccg gtgcggctcc cattccgcgg gcat
//
LOCUS       DBNGSH010000820         2776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3660839, whole genome shotgun sequence.
ACCESSION   DBNGSH010000820 DBNGSH010000000
VERSION     DBNGSH010000820.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3660839"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC16CY_18840"
     CDS             <1..278
                     /locus_tag="AC16CY_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355916.1"
                     /translation="TVRIKIDDDPELVQQWSDSESGQELFAPDGIALARRLADAEHMQ
                     FSFTPYNAGAATASFAVQGAGELVGLVANTCGWRLDGAAAPATRAAR"
     gene            complement(275..>2776)
                     /locus_tag="AC16CY_18845"
     CDS             complement(275..>2776)
                     /locus_tag="AC16CY_18845"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355917.1"
                     /translation="ASLNHPHIAAIHGFDESGGRNFLVMELVEGETLADLIAGASKDA
                     PLRQNVRRGGSSDPPGLAVEEALPIALQIAEALEAAHENGFIHRDLKPANIKVTSDGQ
                     VKLLDFGLAKAFEAQPSEIDVSNSPTLSAMATHAGVILGTAAYMSPEQARGAAVDRRT
                     DIFAFGSVLFEMLTGRRAFEGENVADILAAVLKVEPDWSRLPADVPPAIRRLLRRCLN
                     KDRRRRPQAAGDVRIEIEEADAEPDVSGADHAPPRSGRREHAAWIIAILAASAAVVLA
                     MLAFRPPHESPEMRVEVTTPPTPDPISIAVSPDGRRLAFVASGDGAPRLWLRALDTAA
                     LQPLAGTEGASYPFWSPDGRSIGFFAAGKLKRIEVAGGLPRTLADAAEGRGGTWSVED
                     VIVFSPLPGARLQRVSASSGGDVATVDSGALETPRFPQFLPDGRRFLFFVIGGFGRAT
                     LKGVYLGSLDGSEPRHLTDADAYGLYMPPGWLLYVRGSALMARPFEAARGEFEGEPVV
                     VADPVGVDANVYAAAVSVSAAGLVAYRSGGPSRRQLAWFDRSGRPSGIVGQPDENDLL
                     GPELSPDGRRVAVIRSIRNTRNVWLLDVDRGVSTPFTDDSSSAQNPRWSPDGGRIAFQ
                     SLRTERPGVYIKPLTGTAGDELLVATPTAIPNDWSSDGRFFVYTNNDPKTRGDIWTVP
                     LDGDRTPAPFTNSSYDESSAQLSPDGRWLAYRSDESGRFEIYVQPFPGPGEKTRVSID
                     GGTEPRWRADGREIFYISPDAKMMAVLFQSSGAAPGIGKPVTLFQTRKVRGGTGNVLQ
                     QYDVSPDGRFLINVNADENVASPIMLIMNWKPPAD"
BASE COUNT          466 a          949 c          908 g          453 t
ORIGIN      
        1 ataccgtgcg catcaagatc gacgacgacc ccgaactggt gcagcagtgg tcggattccg
       61 agagcggcca ggagctcttc gcgcccgacg gcatcgcgct cgcgcggcga ctggcagatg
      121 ccgagcacat gcagttcagc ttcacgccgt acaacgccgg cgccgcgacc gcgagcttcg
      181 ccgtgcaggg cgcgggcgag ctcgtcgggc tcgttgcgaa cacctgcggg tggcgcctcg
      241 atggtgccgc cgctcccgcc acacgcgcgg cccgctagtc ggccggcggt ttccagttca
      301 tgatgagcat gatcggcgac gcgacgttct cgtccgcgtt gacgttgatc agaaaccgtc
      361 cgtccggcga cacgtcgtac tgctgcagca cgttgcccgt gccgccccgg accttccgcg
      421 tctgaaagag cgtcacgggc ttaccgatgc cgggcgccgc gccggaggat tggaagagga
      481 cggccatcat cttcgcgtcc ggcgagatgt agaagatctc tctcccgtcg gcccgccagc
      541 gcggttccgt gcccccgtcg attgataccc gggttttctc gccggggccg gggaagggct
      601 gcacgtagat ctcgaatcgc ccggactcgt cggaccgata cgccaaccag cgcccatcgg
      661 gggaaagctg cgcgctgctc tcgtcgtagc tcgagttcgt gaacggcgcg ggtgtgcgat
      721 caccgtcgag cggtaccgtc cagatgtcac cgcgcgtctt cggatcgttg ttcgtgtaga
      781 cgaagaagcg gccgtcggaa gaccagtcgt tcgggatcgc cgtgggcgtc gcgacgagca
      841 gttcgtcgcc ggccgttcct gtcagcggct tgatgtacac accaggccgt tccgtccgca
      901 atgactggaa agcgatgcgg ccgccgtctg gtgaccaacg cgggttctgc gcgctcgacg
      961 aatcgtcggt gaaaggtgtg gacaccccgc ggtcaacgtc gagcagccag acattgcgcg
     1021 tgtttcgaat cgaacgaatc actgccacgc ggcgtccgtc cggcgagagt tccggcccga
     1081 gcaggtcgtt ttcgtccggc tgtccaacta tcccggaagg acggccggaa cgatcgaacc
     1141 acgccaactg ccgccggctc ggtcctccgc ttctgtatgc caccaggcct gccgctgaaa
     1201 ccgacacggc agctgcgtag acattggcgt ccacgccgac tggatccgcg acaaccacgg
     1261 gctcgccttc gaactcgccg cgcgccgcct cgaagggacg ggccatgagg gcgcttcccc
     1321 ggacgtagag cagccagccg ggcggcatgt acaggccata cgcgtccgcg tctgtgagat
     1381 gccgcggctc cgacccgtcg agcgagccga gatagacacc cttgagcgtt gcccggccaa
     1441 agcctccgat gacaaagaag agaaaccgcc ggccgtccgg gaggaactgc gggaaccggg
     1501 gcgtttcgag ggcgccgctg tcgacggtgg caacgtcgcc cccggaggag gccgagacac
     1561 gctgaagacg tgctccgggc agaggcgaga acacgatgac gtcctcgaca ctccacgtac
     1621 cgccccgccc ctccgccgcg tcggcaagcg tacgcggcag accgccggcg acctcgattc
     1681 gcttcagctt gcccgctgcg aagaagccga tcgatcggcc gtccggcgac cagaacgggt
     1741 agctcgctcc ttcggttccg gccagcggct ggagggcggc cgtgtcgagg gcgcgcagcc
     1801 acaggcgcgg agccccgtcg cccgacgcga cgaatgcgag ccggcgtccg tccggcgaga
     1861 ccgcgatcga aatcgggtcc ggcgtcgggg gcgtcgtcac ctccacgcgc atttcaggcg
     1921 actcgtgtgg cggacgaaag gccagcatcg cgaggaccac ggccgccgac gccgcgagaa
     1981 tcgcaatgat ccaggctgca tgttcgcggc gcccggatcg cggaggcgcg tgatcagcgc
     2041 ccgacacgtc cggttccgcg tcggcctctt cgatctcgat tcgaacatcg cccgcggctt
     2101 gcggccggcg gcgccgatcc ttgttcaagc accgccgcag cagccgccgg atcgccggag
     2161 gaacgtctgc cggcaatcga ctccagtccg gctccacctt gagaaccgcc gccagtatgt
     2221 ccgcgacgtt ttctccttcg aacgcccgcc gcccggtcag catctcgaag agcaccgagc
     2281 cgaaagcgaa gatatccgtc cgcctgtcca cggccgcgcc tctggcttgt tccggcgaca
     2341 tgtaggcggc cgtccccaga atcacgccgg cgtgggtagc catggcgctg agcgtcggcg
     2401 agttcgagac gtcgatttcc gacggctgtg cttcgaaggc tttcgccagg ccgaagtcga
     2461 gcagcttgac ctggccgtcg gaggtcacct tgatgttggc cggtttcaaa tcgcggtgaa
     2521 tgaacccgtt ctcatgagcc gcttcgagcg cctccgcgat ctgcagtgca atcggcagtg
     2581 cctcctcgac tgccaaccca ggcgggtctg aagacccgcc tctacggacg ttctgccgta
     2641 gcggcgcgtc tttagacgcg ccggcgatga gatccgccag cgtctctcct tccacgagct
     2701 ccatgacgag gaaattgcgg ccgcccgatt cgtcgaatcc atgaatcgct gcaatgtgag
     2761 gatgattcag cgaggc
//
LOCUS       DBNGSH010000821         2211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_95595, whole genome shotgun sequence.
ACCESSION   DBNGSH010000821 DBNGSH010000000
VERSION     DBNGSH010000821.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_95595"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="AC16CY_18850"
     CDS             complement(<1..721)
                     /locus_tag="AC16CY_18850"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005027052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR3355918.1"
                     /translation="MTFQDLILKLSGYWASHGCLLQQPLDLEVGAGTSHPETLFRVLG
                     PKPWSVAYVQPSRRPDDGRFGQNPNRLFKHHQYQVILKPSPDEVQQLYLDSLEACGIN
                     PRQHDIRFEEDNWESPTLGAWGIGWQVMLDGLEITQFTYFQQVGGIDLSPISCEITYG
                     LERIAMFLQRVDNVFDLEWGGGVTYAEVRLQEEVEQSKYAFGQVDMSGEAFAALHREL
                     FDKTFDFADRLLASGLVWPALE"
     gene            complement(733..1383)
                     /gene="recO"
                     /locus_tag="AC16CY_18855"
     CDS             complement(733..1383)
                     /gene="recO"
                     /locus_tag="AC16CY_18855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR3355919.1"
                     /translation="MPLYTADALVLRTYKLGEADRIVVFLTRDRGKKRGVAKGARRPR
                     SRFTGALEPLTEVTVAYFEKERRELVGLNYAETRRSPLNLDSQDALGHVSYFAELLDE
                     WAQEADADDRLYRLGASMLDALSAGAPVEPLARYFEYWLLRLQGVYPESQGSLSDAAK
                     AFLAGSRAVAPQDVARLSIDRKALRELEGVHRALITTHLEKTLKSDRVLRDLRRHA"
     gene            complement(1427..>2211)
                     /locus_tag="AC16CY_18860"
     CDS             complement(1427..>2211)
                     /locus_tag="AC16CY_18860"
                     /inference="COORDINATES: protein motif:HMM:NF013895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3355920.1"
                     /translation="LVAPDTPLKRIMTADLISARADMDQEEVARQVAAYNLLAIPVVD
                     AENKLVGIITVDDVIDVIKDEATEDIYRLAGVAGDERAFTPAAESLRKRLPWLGVNLV
                     TAFLAASIVGLFEGTIGLFPALAVFMPIVAGMGGNAGTQTLTVIVRGIALGELTWGNS
                     KKALLKEALVGVGNGVALGALAAVVVWVWRGNPVLGLLLGLAMIINMFVAATAGTLVP
                     LGLRAANVDPALASSVFITTMTDMFGFFSFLGLATVFARYLA"
BASE COUNT          392 a          750 c          703 g          366 t
ORIGIN      
        1 gctcgagcgc gggccagacg agcccggaag ccaggagcct gtcggcaaaa tcgaacgtct
       61 tgtcgaacaa ctcgcgatga agcgcggcaa acgcctcgcc ggacatgtct acctgtccga
      121 acgcgtactt cgattgttcg acttcctctt gcaggcgcac ctcggcgtac gtcacgccgc
      181 cgccccactc gaggtcgaac acgttgtcca cccgctgcag gaacatcgcg atccgctcga
      241 ggccgtacgt gatctcgcac gaaatgggcg agagatcgat cccgccgacc tgctggaagt
      301 aggtgaactg cgtgatctcg aggccgtcga gcatcacctg ccacccgatg ccccacgcgc
      361 cgagcgtcgg cgattcccag ttgtcctctt cgaagcggat gtcgtgctga cgcgggttga
      421 tgccgcaggc ctcgaggctg tcgaggtaga gctgctggac ctcgtccggc gacggcttga
      481 ggatgacctg atactggtgg tgcttgaaga gccggttcgg gttctgaccg aagcggccgt
      541 cgtcgggccg gcgcgacggc tgcacgtacg ccacgctcca gggcttggga ccgaggaccc
      601 ggaagagcgt ctccggatgc gacgttccgg cgccgacctc gaggtcgagg ggctgctgca
      661 gcaggcaccc gtgggacgcc cagtatccgg acagcttgag aatcagatct tgaaacgtca
      721 cgctcggctc attcaggcgt gacgcctcag gtcacgaagg acgcggtccg acttcaacgt
      781 cttctccagg tgcgtggtga tgagcgcgcg atgcacgcct tccagctccc tcaatgcctt
      841 ccgatcgatc gacagcctcg ccacgtcctg aggcgcgacg gcgcgcgatc cggccagaaa
      901 ggctttggcc gcgtcggaca ggctcccctg cgactcgggg tacaccccct gcagcctgag
      961 gagccagtat tcgaagtacc gggccagcgg ctcgaccggc gcgccggccg acagcgcgtc
     1021 cagcatcgac gcccccagcc gatacagccg atcgtcggcg tccgcttcct gcgcccactc
     1081 gtcgagcagc tccgcgaagt agctcacgtg cccgagcgcg tcctggctgt cgagattcag
     1141 gggcgagcgg cgggtttccg cgtagttgag gcccaccagc tcccggcgct ccttctcgaa
     1201 gtaggcgacc gtcacctcag tcagcggttc gagcgcgccg gtgaaacgcg atcggggccg
     1261 ccgggcgccc ttggccactc cacgcttctt cccccgatct ctggtcagga agaccacgat
     1321 ccgatcggcc tcaccgagct tgtaggttcg aagaaccagg gcgtctgctg tgtagagcgg
     1381 cactgacgcg cactatttta tgtcggccca gcgtctttca cgcttttcag gccaggtacc
     1441 gggcaaagac ggtggcaagc cccagaaacg agaagaaccc gaacatgtcc gtcatcgtcg
     1501 tgatgaacac cgacgaggcc agcgcgggat cgacgttggc ggctcgcaac ccgagcggta
     1561 cgagcgtgcc ggccgtggcg gccacgaaca tgttgatgat catcgccaag cccagcagga
     1621 gtccgagtac tggatttcct cgccacaccc agacgacgac ggccgcgagg gccccgagcg
     1681 cgacgccgtt gccgacgccc acgagggcct ccttcagaag cgctttcttc gagttgcccc
     1741 acgtcagctc gccaagcgca atcccgcgga cgatgacggt caaggtctgc gtaccggcgt
     1801 tgcctcccat gcccgcgacg atcggcatga agacggccag cgcggggaac aggccgatgg
     1861 tgccttcgaa caacccgacg atcgaggccg ccagaaacgc ggtgaccagg ttgaccccga
     1921 gccacggcag acgctttcga agggattcgg cggccggcgt gaacgcgcgc tcgtcgcccg
     1981 ccacgccggc gagccggtag atatcctcgg tcgcctcgtc cttgatgacg tcgatcacgt
     2041 cgtcaaccgt gatgatgccg acgagcttgt tctcggcgtc cacgaccgga atcgccagca
     2101 ggttgtaggc ggcgacctgc cgcgcgacct cctcctgatc catgtcggcg cgggcgctga
     2161 tcaagtccgc cgtcatgatc cgcttgagcg gcgtatccgg cgccacgagc a
//
LOCUS       DBNGSH010000822         7317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_435435, whole genome shotgun sequence.
ACCESSION   DBNGSH010000822 DBNGSH010000000
VERSION     DBNGSH010000822.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_435435"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="AC16CY_18865"
     CDS             complement(<1..685)
                     /locus_tag="AC16CY_18865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024842.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3355921.1"
                     /translation="MAHDVVIVGGGHNALVAAFYLAKAGRRPLVLERQSRVGGGAITS
                     EIHPGFHCPGLSHELLLEQQIVGDMDLSRRVEFLPSPALACAPQPDGPALVLFDDVAR
                     SAEALRAHSARDAEAYPQFRETIDRIASILGSTFDSPPPDIDRPTAADLWSLLKTGRR
                     FRSLGKRDGYRLLRWIPMPVADLVTEFFESRLLSSALAGPGVSGTMLGPRSAGSSLVM
                     LLREASRQRA"
     gene            769..3747
                     /locus_tag="AC16CY_18870"
     CDS             769..3747
                     /locus_tag="AC16CY_18870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355922.1"
                     /translation="MPLTAGTRLGPHEIVGPLEGGGTGEFYRARDTRLDRTVVIKVLP
                     PHWTGGPESRERLEREAKIIAGLNHPSVRALHDIGRERPQPASPGTVASEVDASGAVE
                     PGADQSPPVAEEPEVDFLVLEHLEGETVADRLARASAGSGVKKAPAFRVDEALSIAIQ
                     IADAIDKAHQKGLVHRDLKPANVFLVKGGKASDPPVAKLLDLGLTGPGPAGFDFAQPR
                     EAGHYVGLDPSTLPTVAPGTVQAPSPSEAEQLDYKAPEQVEGKAADARTDIFAFGALV
                     YEMLTGRRAFEGKSRAVLMAAIMTAEPDPLLALQPQASPALDHVIKRCLAKDPDDRWQ
                     TAHDLLIQLKWVAGIGAKAGRAAAESATARTVRLLVAAAVVMLAAMAGPALLYLRGPQ
                     AVEAFQYRSPVVGLNTADISISPDGRLLAFMARPNQGEPASLFVRPVGSVVSRRLAGT
                     DDAGLPFWSPDSRYIGFVAGGKLKKVDEAGGPPQEIGDAPAFNGGTWNQAGVILFGSP
                     KGLFRVSAEGGKPEALTTLEKGETGHYWPHFLPDGRHYVYLAWSAEAGSRALYTASLD
                     SKERTRLMTAESNVVYAEPGFLTYHREATLFARPFDPDKLTFTGDDLQIAGGLSYNPA
                     TGRGNFDVSQRDALVYFQGDAGGGAAAGRGQTGPTVQFGWVTRTGQQLGPAGVAGVYG
                     DMDLSPDGRFVAVTRQDGPATDIWVIDWQRAAVATRLTLDPGDDINPVWSHDSSRVAF
                     TTFRKGNADIYVKNANGVGDETPLLDSSNNEIIEAWSRDGRYIAYLFGGADNVQDIYA
                     MPLFGDKKPFRVVWGPFQKNEPQFSYDGKWLAYTSNESGSFQVYVISFPALDQRLQIT
                     MAGGGQPRWRQDGRELYYRGLDNRIMAVDFTPGPKIESGAPRFLFAPVVRADNTFNPT
                     RHMLAASPDGQRFLLRIPIVATGTQAGRPQANTTYVPSTGQSGGRGAIFTGAARGGRG
                     FAAGEAGLTVVRNWTTSVAK"
     gene            3754..6621
                     /locus_tag="AC16CY_18875"
     CDS             3754..6621
                     /locus_tag="AC16CY_18875"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3355923.1"
                     /translation="MTIPRGTRLGTFEILDLVGAGGMGEVYRARDTRLERTVAVKVLP
                     PLWAESPDMRQRFEREAQTIASLNHPHICTLHDIGRHQDPASSAPAIDFLVMEFLEGE
                     TLAQRLERGPLGLDEALRVAIAIADALDKAHRKGVTHRDLKPGNIFLVRGGGASDPPI
                     AKLLDFGLAKLKIGRSSGSGGMLAASATPTNPQLTAHGAILGTLQYMAPEQLEGLEAD
                     PRTDIFAFGVVLHEMISGRKTFEGKSRVLLMSAIATAEPPPLAAAQPAVSPALDHVVK
                     TCLAKDPGERWQTARDVLAELQWIAAGGADTIGSTPTSRTRERWSARSLGVVAAAAVL
                     IASLSAVVALYAGSAGEAEEVRFRLPIQLSAQATQVVGTNVVFDPANVSISPDGRSIA
                     YVARGSNSEPWAMYVRPVASVTPRKLPDTDDATQPFWSADGNWIAFVVGGRLRKVEAS
                     GGPPQDIADVAGFSGGDWNIDGTIIFGTPQGLFRVSAQGGKPEPLTTLDPNEAGHFWP
                     HFLPDGRRYLYLAWSGSEGGRAVFAGALDSKEKTRLIPAESKALYAEPGYLVFRREDA
                     VYAQRIDKASLAISGEPVRVADGVTSNGTNGHGDFDVSTTAALVYYRSAGGQSLVGPQ
                     SDGADWQLRWIGRDGQDLEMAGPYGPYRGFELSPDGKRIAVHRHESTGGDVVVIEPRG
                     AVLNLTVDAARHNSMPIWSPDGARVVYSSLQKGKWGLYETLSNGSGTEQLLYESDLLK
                     VPMSWSPDGKRLVFWVQDPKTAGDVWVLTLDDNPSTGSGQPKGAGGWKAAPLINSSYN
                     ESHPQVSRDGKWIAYTSNRTGRNEIYVQPFPTGSGFYKVSVNGGDWARWNPQGKELFF
                     RALGNGAFVGDILVAPYSVSGAAFENGDPKPVVETVGLNLAHSGGDYHMYDVSPDGQR
                     LLVVQFVNTLVTVAVGTNSPDPDFGLIVAQNWTATIEKQGR"
     gene            complement(6769..6915)
                     /locus_tag="AC16CY_18880"
                     /pseudo
     CDS             complement(6769..6915)
                     /locus_tag="AC16CY_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE-like protein"
     gene            complement(7022..>7317)
                     /locus_tag="AC16CY_18885"
     CDS             complement(7022..>7317)
                     /locus_tag="AC16CY_18885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3355924.1"
                     /translation="TVIDTLCEYAPNLRKIILHRQVLTPLDLEREWGLSEGNIFQGEL
                     TLEQLFFLRPAPGWAQYKTPVSGLFMCGSATHPGGGIMGAPGRNAAQRILAEA"
BASE COUNT         1234 a         2453 c         2454 g         1176 t
ORIGIN      
        1 ccgcccgctg ccggctggct tcgcgcagca gcatcacgag cgagctgccc gcggacctcg
       61 ggccgagcat cgtgccggag acgccgggtc cggcgagcgc cgacgagagc agccggctct
      121 cgaagaactc cgtcaccagg tccgccaccg gcatcgggat ccaccgcagc agccggtagc
      181 cgtctcgctt cccgagcgat cggaagcgcc gccccgtttt cagcaggctc cacagatcgg
      241 ccgccgtcgg ccgatcgata tccggaggag gcgagtcgaa cgtcgaaccg aggatcgacg
      301 cgatgcggtc gattgtttcg cgaaactgtg ggtacgcttc cgcgtctcgc gcgctgtgcg
      361 cacgcagcgc ctccgcgctt cgggccacgt cgtcgaacag cacgagtgcc ggaccgtccg
      421 gctgcggtgc gcacgcgagc gcgggcgacg gaaggaattc gactcggcga gacagatcca
      481 tgtcgccgac gatctgctgc tcgagcagca gctcgtgcga cagccccggg cagtggaagc
      541 ccggatggat ctctgacgtg atcgcgccgc cgccaacccg ggattggcgc tcgagcacca
      601 gcggccgtcg gccggctttc gcgaggtaga aggcggccac gagcgcgttg tgcccgccgc
      661 cgacgatgac gacgtcgtgt gccacggttg ggaatcctag caggcccgtg ctcgcgtata
      721 ctctcccggc tgaggctttc ccgcccctgg gaggccttcg gaactaccat gccgctgacg
      781 gccgggacgc gtcttgggcc gcacgagatc gtcggccccc tcgagggcgg gggcacgggt
      841 gagttctatc gcgcgcgcga tacgcggctc gaccggacgg tcgtcatcaa ggttctcccc
      901 ccgcactgga caggcggccc cgaatcgaga gagcgcctcg agcgcgaggc caagatcatc
      961 gccggcctga accacccgtc ggtccgcgcg cttcacgaca tcggtcggga gcgccctcag
     1021 ccggcgtcgc cgggcaccgt tgcgagcgag gttgacgcta gcggggcagt tgagcccggc
     1081 gcggaccagt cgcccccggt cgcggaggaa cccgaggtcg actttctcgt cctcgaacat
     1141 ctcgagggcg agaccgtcgc cgatcgcctg gcgcgcgcga gcgcgggcag cggggtgaag
     1201 aaggcgcccg cgttccgggt ggacgaagcg ctgtccattg cgatacagat tgccgacgca
     1261 atcgacaagg cgcatcagaa aggtctggtt catcgcgacc tgaagccggc caacgtcttc
     1321 ctcgtgaagg gcggcaaggc gtcggaccct cccgttgcga agctgctcga tctgggattg
     1381 acgggtccag gtccggctgg cttcgacttc gctcagccca gggaagccgg acactacgta
     1441 ggactggatc caagcaccct cccgacggtc gcgccgggaa cggtccaagc gccctcgccc
     1501 tccgaggccg aacaactcga ttacaaggcg ccggagcagg tggaaggcaa ggccgccgac
     1561 gcgcgcaccg acatcttcgc cttcggcgca ctcgtctacg agatgctgac ggggcggaga
     1621 gcgttcgagg gaaaaagccg cgccgtgctg atggccgcca tcatgacggc cgagcccgat
     1681 ccgctgctcg cgcttcagcc gcaggcctca cccgcactcg accacgtcat caagcgctgc
     1741 ctcgcgaaag accccgacga ccgctggcaa acggcccacg acctgttgat tcagctgaag
     1801 tgggtcgcag gcatcggagc gaaggcaggc agagccgcag cggagagcgc gactgcgcgc
     1861 acggttcgcc tgctggtggc ggccgctgtc gtgatgctgg ctgcgatggc gggtcccgcg
     1921 ctgctctatc tccgcggacc gcaagccgtc gaggcctttc agtaccgctc gccggtggtc
     1981 ggattgaaca ctgccgacat ctcgatttct ccggacgggc ggctgctcgc cttcatggca
     2041 cggccgaatc agggcgagcc cgcctcgctt ttcgtccgcc ccgtaggttc ggtggtctcc
     2101 cgccggctcg ccggtaccga cgatgcgggg ctgccattct ggtcacctga cagccgctac
     2161 atcgggttcg tcgccggcgg gaagctgaag aaggtcgacg aagcaggcgg tcctccccag
     2221 gaaatcggtg atgcgcccgc gttcaacggc gggacctgga atcaggccgg agtgattctg
     2281 ttcggctccc ccaaagggtt gttccgcgtg tcggcagaag gcggaaagcc agaggctctt
     2341 actacgctcg agaagggtga gaccggacac tactggcctc acttcctccc cgacggccgc
     2401 cactacgtat atctcgcgtg gtcggccgag gccggcagcc gggcgctcta taccgcatcg
     2461 ctcgattcga aagagcgcac gcgcctgatg accgccgagt cgaacgtcgt ctacgccgag
     2521 ccgggttttc tcacctacca ccgggaggcc acgctctttg cgcggccttt cgatcccgac
     2581 aagctgacgt tcaccggtga cgacctgcag attgccggcg gcttgtcgta caacccggca
     2641 accggccgcg gcaacttcga cgtgtcgcag cgcgacgcgc tcgtgtactt ccagggcgat
     2701 gcgggcggcg gagccgcggc tggccgcggg cagactggtc ccaccgtgca gtttggctgg
     2761 gtcacacgaa cgggccagca gctcgggccc gccggcgtcg cgggggtcta tggtgacatg
     2821 gacctttcgc ccgacggcag gttcgtggca gtgacccggc aggatggtcc cgccaccgac
     2881 atctgggtga tcgactggca gcgcgcggcc gtggcgacgc ggttgacgct cgaccccggc
     2941 gacgacatca accccgtctg gtcgcacgat agctctcgtg tggctttcac gaccttccgg
     3001 aaggggaacg cggacatcta cgtgaagaac gccaatggcg tcggcgacga gacaccgctc
     3061 ctcgactcat cgaacaacga gatcatcgag gcctggtcgc gcgatggcag gtacatcgcg
     3121 tatctcttcg gcggcgcgga caacgtccag gacatctacg caatgccgct cttcggcgac
     3181 aagaagccgt tccgtgtcgt gtggggcccc ttccagaaga acgagccgca gttctcgtac
     3241 gacggaaagt ggctcgccta cacctccaac gagtcgggct cgttccaggt gtatgtcatc
     3301 tcgttcccgg cgcttgacca gcggctgcag atcacgatgg cgggcggcgg gcagccccgc
     3361 tggcgtcagg atggacgcga actctactac cgggggctgg acaaccgcat catggccgtg
     3421 gacttcaccc ctggtccgaa aattgagtcg ggagcgccac gattcctgtt cgcccccgtc
     3481 gtgcgggccg acaacacatt caaccctact cgtcacatgc tggctgcgag cccagacggc
     3541 cagcggttcc tcctgcgcat ccccatcgtc gcgactggaa ctcaggcggg aagaccccag
     3601 gcgaatacca cctacgtgcc atcgacggga caatccggcg gcaggggggc catcttcacg
     3661 ggcgccgccc gcggggggag gggtttcgcc gcgggcgagg ccgggctgac agtcgtccgc
     3721 aactggacga cgtctgtggc gaagtagagc gacatgacga tcccgcgcgg cacgcgtctc
     3781 ggcacgttcg agattctcga cctagtgggc gccgggggga tgggcgaggt ctatcgggcg
     3841 cgcgatacac ggctcgagcg cacggtggcc gtcaaggtcc tcccgccgct ctgggccgag
     3901 agcccggaca tgcgtcagcg cttcgagcgc gaggcgcaga cgatcgcgtc gctgaaccac
     3961 ccgcacatct gcacgcttca cgacatcggc cggcaccagg atccagcgag ctcggccccg
     4021 gcgatcgatt tcctcgtgat ggaattcctc gagggcgaga cgctggccca gcggctcgaa
     4081 cgcgggccgc tcggcctgga cgaggcattg agggtagcga ttgcgattgc cgacgccctc
     4141 gacaaggcgc atcgcaaggg ggtcacgcac cgcgatctca agccgggcaa catcttcctc
     4201 gtgcgcggcg gcggtgcatc ggatccgccg atcgccaagc tgctcgactt cgggctcgcg
     4261 aagctcaaga tcggccgatc gagcgggtcc ggcgggatgc tggccgcgtc cgcaacgccg
     4321 accaacccgc agctgaccgc gcacggcgcc atcctcggga cgctccagta catggcgccc
     4381 gagcagctcg agggtctcga agccgaccca cggaccgaca tctttgcgtt cggcgtcgtc
     4441 ctgcacgaga tgatcagcgg cagaaagacg ttcgagggca agagccgggt gctgctgatg
     4501 tcggcgatcg ccaccgccga gccgcctccc cttgccgcag cgcagccggc cgtctcgccg
     4561 gcgctggacc acgtggtgaa aacgtgcctg gccaaggatc ccggcgagcg ttggcagacg
     4621 gcgcgcgatg tgctcgccga gctgcaatgg attgccgcgg gcggcgccga caccattggc
     4681 tcgacgccga catcgcgcac gcgcgagagg tggagcgcgc ggtctctcgg cgtggtggcg
     4741 gctgccgcgg tcctgattgc ctcgctgtcg gccgtcgtcg ccctttacgc cggaagcgcc
     4801 ggtgaggccg aagaggtccg cttccggttg cccattcaac tgagcgccca ggccactcag
     4861 gtcgtcggca cgaacgtcgt gttcgatccg gcgaacgtct cgatctcgcc cgacggccgc
     4921 tcgattgcct acgtggcaag ggggtcgaac agcgagccct gggccatgta cgtccgaccc
     4981 gtcgcaagcg tcactcctcg gaaactcccc gacaccgacg acgcgacaca acccttctgg
     5041 tcggccgacg gcaactggat tgccttcgtc gttggcggaa ggcttcgcaa ggtagaggcc
     5101 tccggaggcc cgccgcagga catcgccgac gtggcgggct tctcgggcgg cgactggaac
     5161 atcgacggga cgatcatctt tggcacgccg caaggactct tccgcgtgtc ggcccagggt
     5221 ggcaagcctg agcctctcac gacgctggat ccgaacgagg ccgggcactt ctggccgcac
     5281 ttcctcccag atggccgccg ttacctctac ctggcatggt ccggttcgga aggtggtcgc
     5341 gcagtcttcg cgggagcgct cgactcgaag gagaagaccc gcctcattcc cgccgagtcg
     5401 aaggcgttgt acgccgagcc gggctacctg gtgttccggc gagaagacgc cgtctacgcg
     5461 cagcgcatcg acaaggcaag cctggccatt tctggcgagc cggtgcgtgt ggcggatggg
     5521 gtcacctcga acggcaccaa tggccacggt gacttcgacg tgtcgacgac tgccgcgttg
     5581 gtctactacc ggtcggccgg cggccagagt ctcgtcggcc ctcaatcgga cggggccgac
     5641 tggcagttgc ggtggatcgg tcgtgacggt caggacctcg agatggccgg accctatggt
     5701 ccatatcgcg ggttcgagct gtcgccggac ggcaaacgaa ttgcggttca tcggcacgaa
     5761 tcgaccggcg gcgacgtcgt cgtgatcgag ccgcgcggtg ccgtgctgaa tctgaccgtc
     5821 gacgcggcgc ggcacaactc gatgcccatc tggtcgcccg acggcgcccg ggtggtctac
     5881 agctcgctgc agaaggggaa gtgggggctc tacgagacgc tctcgaatgg atcgggtacc
     5941 gagcaactgt tgtacgaatc ggatttgctc aaggttccga tgtcgtggtc gccggacggc
     6001 aagcgcctcg tgttctgggt gcaggacccg aagaccgcgg gtgacgtgtg ggtgctgaca
     6061 ctcgacgaca acccctcgac aggctcggga cagccgaagg gcgctggcgg ctggaaggcg
     6121 gcgccgctga tcaactcgtc gtacaacgag agccacccgc aggtttcgcg cgacgggaag
     6181 tggattgcgt acacctccaa caggaccggg cgcaacgaga tctacgtgca gcctttcccg
     6241 acgggcagcg gtttctacaa agtatcggtc aacggcgggg actgggcccg gtggaatcct
     6301 caaggcaagg agttgttctt ccgcgcgctc ggaaacgggg ccttcgtcgg cgacatcctc
     6361 gttgcgccct acagtgtcag cggcgccgcg ttcgagaatg gcgaccccaa accggtggtc
     6421 gagaccgtcg gactcaacct ggcccacagc ggcggcgact atcacatgta cgatgtgtca
     6481 cccgatggtc agcgtctcct cgtcgtgcag ttcgtcaaca cgctcgtgac cgtcgcagtc
     6541 ggcaccaaca gcccggaccc agacttcggc ctcatcgtcg cgcagaactg gaccgccacg
     6601 atcgagaaac agggtcgata ggctctgggc tctcggcttt cggctctggg gctcggcacc
     6661 gcgtgcccgc ctacggttct ccggcgggag cctcggcgag accgagaaag acgaagattg
     6721 cccgattgag gtgtctcagg tcttcgggag ccagagaacc gacgcgtcgt ccgactggcg
     6781 gtgcgcagcc cgggcgaacg atgtggatcg gacatcggga acccagatct ggaccggacg
     6841 aagtccctgc ttccggagcc gcgctcggtg cgcgcggacc ttgtcgcgcg aagcggatcg
     6901 agcagccgtg cccatcactc tcctccagag gttacatgta acttggcaca ggcgcgggtc
     6961 cggccaaagc cggacgctac gtactcacgg tccggctgaa gccggacact acgtaccttg
     7021 attacgcttc ggcgaggatc cgctgcgccg cgttccttcc cggcgcgccc atgatgcccc
     7081 cgcccgggtg tgtcgccgac ccgcacatga agagcccgct taccggcgtc ttgtactgcg
     7141 cccagccggg cgcgggacgc aggaagaaca gctgctccag cgtcagctcg ccctggaaga
     7201 tgttgccctc cgagagtccc cactcccgct cgaggtcgag cggtgtcagc acctgccgat
     7261 gaagaatgat cttccgcaga ttcggcgcgt actcgcacag cgtgtcgatc accgtgt
//
LOCUS       DBNGSH010000823         4028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_95654, whole genome shotgun sequence.
ACCESSION   DBNGSH010000823 DBNGSH010000000
VERSION     DBNGSH010000823.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_95654"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="AC16CY_18890"
     CDS             complement(<1..534)
                     /locus_tag="AC16CY_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355925.1"
                     /translation="MKSKGVVILAVIAVAALAWWWIRSRVDVRFASDGMDFARLEHEY
                     PLSREEREWFTPERLAAYSQEQVDQVYGRLTAGPIPDGAYDGDLFFPRGTDGEARLAE
                     IIAGHLQSRIANLGVRKTEGLGRLLWKGKVFYRDQRLLRNRIEDLAILAPLTGGNTDG
                     IEKLSVNGRDTFLLFPAR"
     gene            complement(558..2996)
                     /locus_tag="AC16CY_18895"
     CDS             complement(558..2996)
                     /locus_tag="AC16CY_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR3355926.1"
                     /translation="MASKLRSLALALASLALLLVILRVATSQPAPPQVLDPGFHAGAQ
                     GLSPSARAGREIWFKATAGNDRFHTYVFQQRLGVMIDWYRVLRSEARGDRFKTWGIVN
                     DPDCCTPGSPDCPRKSLDETYGFDYCPGDDALLSFVGKPGYRDPACDFQDAPMSDKDP
                     HGPKDQRQSACDLAFGTSTGALGFRKFPNPRFDAGRWRSLNGSMATWSGFNSPLKPDG
                     ADSRTNRLTDGSIEPPFLIGMSCGACHISFNPLKPPADPEHPAWENIDGLVGNQYGRI
                     SEIFASGMSPSSIEWQTFAHARPGTVDTSGIPNDSVSNPGTMNPIMNLARRPTFEEEV
                     TKWRPVTSCAAGADQRACWCEPGRDGKCWERSTKTEPVHHILKGTEDSIGALEAIQRV
                     YFNIGSCSEQCWVNHVTDLRQADPRHRGFGQTPFDVGQCRRDCDNFRAIEDRLQNIAD
                     FFMTSRPVDLYRARDLPSQAALIAQLDREFGANAVARGKAIFASTCAQCHSSQPGPWT
                     EATDFHATVEGQPDLRLDFLSNERPVPVTQVGTSRSRALHSNHMKGRVWEEYGSETLR
                     ARPPVDGIPEPHDGGRGYYRPISLLSVWAFAPFMHNNAIGPEVCGRPTDPLELYRSPY
                     VVRGSWRRMPNPPPCVTFDPSVEGRYRLFKASVEELLNPSARVPKITLVDQPIIIDGP
                     IFPGESQGFELQIPAGIPAAIPANLRHKELIADLVLARANLEGLRIKYAARGPADADR
                     IVTTLQQILKQLTAGLVTSPDSIMSTIGREHLPFIQQMYSNSTVEVENDGHTFGSTLS
                     ADDKKALTAFLATL"
     gene            complement(3097..3909)
                     /locus_tag="AC16CY_18900"
     CDS             complement(3097..3909)
                     /locus_tag="AC16CY_18900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3355927.1"
                     /translation="MRIRPLVGALCLIAGLATARGQEAAFEVASVKINTSDAPVSRIS
                     APAGTGRFEATNASVRMLILNAYGIPGFQLVGGPSWVDSLNVDVIARGAASATRAEIS
                     AMLRTLLAERFNLAVRRDTREMPVYALVVARDDRRLGPRMQASTTDCAAAAAPGSLAP
                     QTASGQLLCTTRMSPFTINAGGMTMVRLAQSLSGIVDRVVTDETKLSGGYDLQLSFTP
                     ERPPPPGASPPADPDAPSIFAALQEQLGLKLDARRGSVEMLVIDRIDRPKEN"
BASE COUNT          637 a         1327 c         1374 g          690 t
ORIGIN      
        1 cctcgccggg aacagcagga acgtgtcgcg cccgttcacc gacagcttct cgatgccgtc
       61 ggtgttccca ccggtcaacg gcgccaggat ggccaggtcc tcgatgcggt tgcggagcag
      121 gcgctggtcg cgatagaaca cctttccttt ccagagcagg cgtccaagtc cctcggtctt
      181 gcgcaccccg aggttggcga tccgggactg aagatgtccc gcgatgatct cggccagccg
      241 cgcttcgccg tccgtgccgc gcgggaagaa gaggtccccg tcgtaggcgc cgtccgggat
      301 cgggccggcg gtgagacggc cgtagacctg atcgacctgt tcctgcgagt acgccgcaag
      361 ccgctcgggg gtaaaccact cgcgctcctc gcgcgacagc ggatactcgt gctccagccg
      421 cgcgaagtcc atcccgtcgc tggcgaaacg cacgtcgact cgtgagcgga tccaccacca
      481 cgccagcgcc gcgacggcga tgacggccag aatgacgacg cccttggact tcacagccag
      541 cctcctctac agcgtctcta cagtgtcgcg aggaaggcag tcagcgcctt cttgtcgtcg
      601 gccgacagcg tgctgccgaa cgtgtggccg tcgttctcca cctcgacggt gctgttgctg
      661 tacatctgct ggatgaatgg caggtgctcg cggccaatcg tcgacatgat cgagtccggg
      721 ctcgtgacca ggcccgcggt cagctgcttc aggatctgct gaagcgtggt gacgatgcga
      781 tcggcgtcag cggggccgcg cgcggcgtac ttgatccgca gcccctcgag gttcgctctc
      841 gcgagcacga ggtcggcgat cagctccttg tgccggaggt tggcgggtat cgcggcagga
      901 atgccggcgg ggatctgcag ctcgaaccct tgcgactctc ccgggaagat cggtccatcg
      961 atgatgatcg gctgatcgac gagcgtgatc ttggggacac gggcggaagg gttgagcagc
     1021 tcttccaccg atgccttgaa cagccggtat cgcccttcga cgctgggatc gaacgtcacg
     1081 cacggcggcg gattcggcat ccgcctccag ctcccgcgca ccacgtacgg gcttcgatac
     1141 agctcgaggg ggtcggtcgg cctgccgcac acctcgggac cgatcgcgtt gttgtgcatg
     1201 aacggggcga aggcccagac gctcaggagc gagatcggac ggtagtagcc gcggccgccg
     1261 tcgtgcggct caggaatccc atcgaccggg ggcctcgcgc gcagcgtctc ggagccgtat
     1321 tcctcccaga cgcgcccctt catgtgattc gagtgcagcg cgcgcgacct cgacgtgccg
     1381 acctgcgtga ccggcaccgg ccgctcgttg ctgaggaaat ccagccgcag atccggctgg
     1441 ccctcgaccg tggcgtgaaa gtcggtcgcc tcggtccatg gccccggctg gctcgaatga
     1501 cactgcgcgc acgtggaggc gaagatggcc ttgccgcggg ccacggcatt ggcgccgaac
     1561 tcccgatcga gctgggcgat gagcgccgcc tgcgagggca ggtcgcgcgc gcggtacagg
     1621 tccacgggcc ggctggtcat gaagaaatcg gcgatgttct gcagcctgtc ttcgatcgcg
     1681 cggaagttgt cgcagtcgcg ccggcactgg ccgacgtcga acggcgtctg gccgaagccg
     1741 cgatgcctgg ggtcggcctg gcgcagatcg gtgacgtggt tgacccagca ctgctcggaa
     1801 cacgaaccga tgttgaagta gacgcgctgg atcgcctcga gcgcgccaat gctgtcctcg
     1861 gtccccttga ggatgtggtg aaccggctcg gttttcgtcg atcgctccca gcactttccg
     1921 tcgcgtcccg gctcgcacca gcacgcgcgc tggtccgcgc cggcggcgca cgaggtgacg
     1981 gggcgccact tcgtcacctc ctcttcgaac gtcgggcggc gcgccaggtt catgatcggg
     2041 ttcatcgtgc ccgggttgct caccgagtcg ttcgggatcc cggacgtgtc taccgttccc
     2101 ggccgggcat gggcgaacgt ctgccactcg atgctgctcg gagacatccc cgacgcaaag
     2161 atctcggaga tgcggccgta ctggttgccg acgaggccgt cgatgttctc ccaggcaggg
     2221 tgttccggat cggccggtgg cttcagcggg ttgaacgaga tgtggcaggc gccgcaactc
     2281 atcccgatga gaaacggcgg ctcgatcgag ccgtcggtca ggcgattggt gcgcgagtcg
     2341 gcgccgtcgg gcttcagcgg actgttgaag ccggaccacg tcgccatcga gccgttcagc
     2401 gatcgccagc gcccggcgtc gaatcgaggg ttggggaact ttctgaaccc gagggcaccg
     2461 gtggacgtgc cgaacgccag gtcacaggcg ctttgccgct ggtccttggg accatgcggg
     2521 tccttgtcac tcatcggtgc gtcctggaag tcgcaggcgg gatctctgta tcccggtttg
     2581 ccgacgaacg acagcagcgc gtcgtcgccc gggcagtaat cgaacccgta ggtctcgtcg
     2641 aggctcttcc ggggacagtc cggcgatccg ggcgtgcagc aatccgggtc gttgacgatg
     2701 ccccatgtct tgaaacgatc gccgcgcgcc tccgagcgca ggacgcgata ccagtcgatc
     2761 atcacgccga ggcgctgctg gaaaacgtag gtgtggaagc ggtcgtttcc tgcggttgcc
     2821 ttgaaccaga tctcccggcc ggcgcgcgcc gatggactga gtccctgtgc gccggcgtgg
     2881 aatccaggat cgaggacctg cggtggcgcc ggctgcgagg tggcgacgcg aaggatcacc
     2941 agcagaagcg cgagcgaagc aagggccagc gcgaggctgc gcagcttcga tgccatggca
     3001 gtatcccccc cgaatccgcg gatgttaact cactccttcg aggatgagct tcgcccgttc
     3061 gggatcaagg cgggtctgcc gaggccatcg aacctgtcag ttctccttcg gccgatcgat
     3121 gcgatcgatc accagcattt ctacgctccc gcgccgcgca tcgagcttga ggccgagctg
     3181 ctcctgcagc gcggcaaaga tcgacggcgc atcgggatcc gccggtggcg acgcgccggg
     3241 cggcggcgga cgctcagggg tgaacgagag ctgcaggtcg tagcctcctg acaacttcgt
     3301 ctcgtccgtc accacgcggt cgacgatgcc gctgagcgat tgcgccagcc gcaccatcgt
     3361 cattccgccg gcgttgatcg taaacggcga catccgcgtg gtgcagagca actgcccgga
     3421 tgcggtttgc ggtgccaggc ttccaggtgc cgcggcggcg gcgcagtctg tcgtcgaggc
     3481 ctgcatgcgc ggcccgagcc gtcgatcgtc gcgcgcgacg accagcgcgt acaccggcat
     3541 ctcgcgcgtg tcgcgtcgca cggcgagatt gaagcgctcg gcgagcagcg tgcgcagcat
     3601 cgcggagatc tccgcccggg tggcgctggc ggcgccgcgg gcgatgacgt ccacgttcag
     3661 ggagtccacc caggacggcc cgccaacgag ctggaacccg gggatcccgt acgcgttcag
     3721 aatcagcatt cgcaccgacg cgttggtcgc ctcgaaccgg ccggtgccag caggcgcgct
     3781 gatgcgcgag acgggagcgt ccgacgtgtt gatcttgacg gaggccacct cgaaggccgc
     3841 ctcctgtcca cgagccgtgg cgaggccggc gatcaggcaa agcgctccaa caagtggccg
     3901 gatgcgcatc tctcctccgt agaccatcac tacagccctt ggacggaggc gggcgctcgc
     3961 cggttagcct gcgggccctc tttcgcgtga cgccttcagt cggccggctc gatccggagc
     4021 aatgcccc
//
LOCUS       DBNGSH010000824        10823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1283831, whole genome shotgun sequence.
ACCESSION   DBNGSH010000824 DBNGSH010000000
VERSION     DBNGSH010000824.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1283831"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="AC16CY_18905"
     CDS             <1..214
                     /locus_tag="AC16CY_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355928.1"
                     /translation="GIAKGNLTGETKISDGKVDGQKITFVENATFMEMPVRFEYSGTM
                     TSADEIKMSRKVLDIATEELVAKRVK"
     gene            304..855
                     /locus_tag="AC16CY_18910"
     CDS             304..855
                     /locus_tag="AC16CY_18910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3355929.1"
                     /translation="MAAARMGTDDDFAELVRAHRVRVIGLCRRLLGPGDQAEDAAHEV
                     FLRAHTRRQDFDRSRPFSSWVLSIAAHYCVDLLRRRSTESRIFGTEEAERIAVASDRV
                     SPLGLLLLRERQQEVRDAIRSLPDKYRVPLVLSAYHEQTYDEIAQALGVPRSHVAMLL
                     FRARQQLRRALSRPLDANNGSRP"
     gene            852..2486
                     /locus_tag="AC16CY_18915"
     CDS             852..2486
                     /locus_tag="AC16CY_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355930.1"
                     /translation="MTCCDELTLMMYADGELEDGPRREVGVHVDQCRRCGSLLAALHV
                     ESRVVAIAVRDEDTASAAVARPVPGRQTSAALRVAAAAASVALGAALLRFLVESAGLA
                     PLAALPDWLNPLSSQGQSNLLFNTAAYLVLEGDLIMSLVMGSASVLALAVLALSFAPA
                     AGRLARGRSVLASLVLLSVLLPAAAADALEVRRAERTLTIASDQTIDDTVIAAAETVE
                     VDGTITGDLIAVGRRVRVRGMVRGNLITAAQNIEIEGTVDGSVLQFGQTVTNRGQTGG
                     NLYAFGQTITTPGVARVGGNVTVFSETATIEGSVGHDVTSFGRQLDLGGSVGRRVNAY
                     GDRVDIRSSARIDGGLTAHVANRENVRVDPGATVGGETRIDVTEPSPSRFATSSFYVR
                     QVIRLAAAFIAGWIFFRLVPRAAAITLGTGGAALTALGIGAVAACATPVLAIVVGITI
                     VGLPIAVFAVLLWIVLLYLAKIVIALLVGRALVGASARGIATALLAGLVLVIVAVNLP
                     YVGGLVSLLLTVTGLGVLVVEASRWYRGSGGVPVRA"
     gene            complement(2460..3269)
                     /locus_tag="AC16CY_18920"
     CDS             complement(2460..3269)
                     /locus_tag="AC16CY_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355931.1"
                     /translation="MPRNVEVCRCGSERRRLEALGYTFDSTPAHAGSQAPSAPRQPRE
                     RRGLLTALIGYEIDTDLSAGWRGTYKGLFVVAVVAVFGFTARFVHTHRQPVRGTVDVI
                     VSLQDFTRSVDPNVKNAIPSFLASAGRVGSLPLTGTPTDPVRPIDEAELRQGFCSPRL
                     ATLVRYEYPGYYDDWSDEELEQVVLEKYPDYSDRLCMLSSRLDAGPGEVVKYEFKPRP
                     PQSWALVGLSALLPTAVFALACLNVYYRVIVGRRAARRERDQGSRTNRNAA"
     gene            complement(3344..4264)
                     /locus_tag="AC16CY_18925"
     CDS             complement(3344..4264)
                     /locus_tag="AC16CY_18925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HIR3355932.1"
                     /translation="MPRRRLVVLTSVAAVLAAGCANMPAPAVVPTAPLASDVVADART
                     LDQPSNAARVDAVQSLLRRRGLPFTLQTFPNTSRQRDPREQGTNVIVDLPGPAGPEII
                     VGAHLDAAALKAGGYSHGMVDNGAGVVVLVRAAEALRARRLRHRVRIVFFDMEEIGLA
                     GSRHFASLLDKAGVAAMVNVDIAGYGDTILSGAAPGAESLRHSLARVCAARNYACMAF
                     PSLPNSDDRSFQAAGIPAISLAVLPALEAHQIWLLLNGGKESGLTPGFAPSILRTIHT
                     PEDTADKLTPQGMTLIHDTVVGLVLELDAR"
     gene            complement(4294..5277)
                     /locus_tag="AC16CY_18930"
     CDS             complement(4294..5277)
                     /locus_tag="AC16CY_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3355933.1"
                     /translation="MPPKPLTGHVAVVAGATRGAGRGIARALGEAGATVYCTGRSVKG
                     QPSPYKRPETIDETAEMINDAGGTAIPVRVDHTIEAEVEALFARIDREHQRLDIVADS
                     VAGEDPMMGQYGNFWQVDLKDADAGFRQALTSHIITAKHAAPFMMRHRRGLIVQVTEN
                     DLLTAGGNPVSQSVKVALRVLALTMAAELKEHGVSALAITPGFLRSESMLEHHGVTEA
                     NWRDAGKQDPNFLESESPLFVGRAVAALAGDPDILTQTGQLFSSWEVARRYGFTDYDG
                     RRPDWGRLAIDWSVLPPPLVDLIRTGTQLQIAWLTTLAERTRNFLDKIPPA"
     gene            complement(5286..6260)
                     /locus_tag="AC16CY_18935"
     CDS             complement(5286..6260)
                     /locus_tag="AC16CY_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3355934.1"
                     /translation="MTKPLTGHVAVVAGAARGAGRGIARMLGEAGATVYCTGRSSRTQ
                     PNTSNHVHARRPETIEETAEMVTAAGGTGIPVRVDHEVGEEVAALFARVRRERKRLDV
                     LATVITGQPASWKNFLNESPDSGRAFVESWIWPHIVTSWHAAKMMVKRRSGLIVELVE
                     HDNVGYHGAFYFDVMETLLKRLVFGLANDLGKQGVTAVAIGPGFMRTEAILDGFGVTE
                     ANWRDAVKSPQAAAMGWGGSETPCFVGRAVAALAADPDVARKNGGIYTAHALSEEYGF
                     TDIDGAPPDHAVLTAAFEQAKSTFLAPMIEATRSVRVDWKVSPKTGEL"
     gene            6336..6959
                     /locus_tag="AC16CY_18940"
     CDS             6336..6959
                     /locus_tag="AC16CY_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3355935.1"
                     /translation="MSPRPRSTSDAEILSATHRAVTRVGPKRLTLADVAREAGVSPAT
                     LVQRFGSKRKLLLAFVATGSSEIEAEWARILSLAERSPLEALYAYAECMAGLAPNPDV
                     LANHMSFLQMDLVDPEFHHHALAQARHTLEFLRKTLDDAVDRGELAPCDTTRLARAVQ
                     ATIGGSLMQWAIDREGKLRRRLREDLETLLQPRYPRRAKRPHARRRR"
     gene            complement(7033..8289)
                     /locus_tag="AC16CY_18945"
     CDS             complement(7033..8289)
                     /locus_tag="AC16CY_18945"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3355936.1"
                     /translation="MTAGTVAGPGPPASRGLARLGAALGHRDFRILWCGAFLSTIGTW
                     VQKVAQNWLVLTITGTSSAFFLGLDSFLGELPILLFTLVGGVIADRHDRRRLLLASQC
                     VQMGAAFTLAALVYWDLVRIWHVLALSVVTGLAQAFGGPAYQSLLPSLVSKDHVPNAI
                     AFNSIQFNLARVIGPLLAGAALAAFGMVACFGLNGLSFVAVIAAILSLHVRHTPPAST
                     TRLHEEFKSGLSYVGSHPALVGLALLGFAATFLGSPVLTFLPLFAQDVLGGGVAQYTH
                     LMSSAGAGAVTGALVVAWRGRFPHMGRTCLLIQIAFGAFVALFAVTRIFWLNAALLFA
                     AGACMVMAFATLSSLVQLNAPNQMRGRVMSIYLVAFRGGMPLGSLAGGWLATLTSAPA
                     VLTVSGLTLSAVAAWVLATNRPIREL"
     gene            8370..8765
                     /locus_tag="AC16CY_18950"
     CDS             8370..8765
                     /locus_tag="AC16CY_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355937.1"
                     /translation="MRPLAPRALGGLVLLWALVSVVSAWQAAAARGPDRVTALEAGFR
                     ALAASAPASGDIGFLRHYEHPDSPDDIRDYYVAQYAFAPRLVARRTDVDLLIVAQGAA
                     HPDRDERLAGFALVASSPEGHRLYRKLIR"
     gene            8762..10141
                     /locus_tag="AC16CY_18955"
     CDS             8762..10141
                     /locus_tag="AC16CY_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355938.1"
                     /translation="MIREAIALLVPASAIVLFAPSLRSACGRSRAAAAALALCLGIGV
                     SSVTTAGLIASGLTPRDGIFVVTDVVIWAIVGTTGWWLWRRNARSVSHTHPPVHARSL
                     NGLDWIVRLVFAAVAAIALGVVATSFRVAPHGDWDAWAIWNLHARFLFSGGDRWRDLL
                     MVDWSQPGYPLLLPASVARVWAYAGHDTTAGPAIVAIVFGVTTVTIVMSTIGLERPRA
                     WIAGALLAGAGSFLVQIPSLCADVPIACFMVVSLAAACGREGVLAGRETSRTAALVSG
                     GAGAMAAWTKNEGLVFVLVMLLLVAVVTVRRGGGRWLSWWIAGGAPMLAALAWFKLAL
                     APPSSWFAQSPLVYAGLLSDTERYAVTASLMAQHLWRWGAPLAGIIPLLALAAVWLSV
                     VGGGAMRMMAALVALMFVGYCLAYLTTPFDITWHVTTSFDRLLVQVWPSLVLTVFFDE
                     TRLNRLEYA"
     gene            10214..>10823
                     /locus_tag="AC16CY_18960"
     CDS             10214..>10823
                     /locus_tag="AC16CY_18960"
                     /inference="COORDINATES: protein motif:HMM:NF012381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR3355939.1"
                     /translation="MTNPAVAVLQSALRARKLDRTLTDALPTLERADPSSLAPMDVAS
                     IDACLHGGLPRGQLSELSGPCSSGRTTLLLQFMAAATRRGEIAAYVDTFDRLDVASAA
                     AAGVDLDRLLWIRGQSVIGDHKPRSHGGAEENNQESPCLRVSVVRDRALDRALKALNL
                     VLQAGGFGIVAIDLADAPPRELRQIPFTTWPRVQRAIEGRDTA"
BASE COUNT         1652 a         3640 c         3697 g         1834 t
ORIGIN      
        1 cgggattgcc aagggcaacc tgacggggga gacgaagatc tcggacggta aggtggacgg
       61 ccagaagatc acgttcgtcg agaacgcgac gttcatggag atgcccgtcc gattcgagta
      121 ctccggaacg atgacctctg ccgacgagat caagatgtcc cggaaggtcc tcgacatcgc
      181 gaccgaggag ctcgtcgcca agcgcgtgaa atagtgagaa ggggtcagac cccttcccac
      241 gatttcccag gttgcgtttc gcaattccgc cacgcctcct tcgtaatacc tctgtgtatg
      301 gccttggccg ccgcacggat gggaaccgac gacgacttcg ctgagctcgt ccgggcccat
      361 cgcgtcaggg tcattggcct ctgccgccgt ctcctgggac caggggacca ggcggaagac
      421 gccgcccacg aagtctttct tcgcgcacac acgcggcggc aggatttcga cagaagccgc
      481 ccgttttcga gctgggtgct gagcatcgcc gcccactact gcgtcgatct gctccggaga
      541 cgaagcaccg aatcgaggat cttcgggacg gaagaggccg agcggatcgc cgtcgcttcc
      601 gaccgtgtca gtccgctcgg gctcctgctc ctgcgagagc gccagcagga agtgcgggac
      661 gcaatccggt cgctgcccga caagtaccgc gtgccgctcg tgctgtcggc gtaccacgaa
      721 cagacctacg acgagattgc gcaggcgctg ggcgtgcccc gctctcacgt cgcgatgctc
      781 ctgttccggg cccggcagca gctccggcgc gcgctgtcgc gtcccctcga tgccaacaac
      841 gggagtcgcc catgacctgc tgtgacgaac tgacgctcat gatgtatgcc gacggcgagc
      901 tggaggacgg acctcgccgt gaggttggcg ttcacgtgga ccagtgccgg cggtgcgggt
      961 cgctgctggc ggcgctgcac gtcgagtcgc gtgtggtcgc gatcgccgtc cgcgatgagg
     1021 acaccgcctc cgccgcggtc gcccgcccgg tcccggggcg gcagacgtct gcggccctgc
     1081 gcgtggctgc cgccgcggcg tcggtcgctc tcggggcagc gctcctccgg tttctcgtcg
     1141 aatcggccgg cctggctccg cttgcggcgc tgcccgactg gttgaacccg ttgagcagcc
     1201 agggacagtc gaaccttctc ttcaacaccg ccgcgtacct tgttctggaa ggagatctca
     1261 tcatgtcgtt ggtcatgggc tcggcgagcg tgcttgcact tgccgtgctg gcgttgtcgt
     1321 tcgcaccggc ggccggcagg cttgcgcgag ggcgaagcgt cctcgcgagc ctggtcctgc
     1381 tctcggtgct gttgcctgcg gcggccgctg atgcgctgga ggtccggcgc gcggaacgga
     1441 ccctgacgat tgcgtccgac cagaccatcg acgacaccgt cattgcggcc gcggagaccg
     1501 tcgaagtcga cgggaccatt actggcgacc tgatcgcggt cgggcgccgg gtccgcgtgc
     1561 gcgggatggt ccggggcaat ctcatcacgg cggcgcagaa catcgaaatc gagggcaccg
     1621 ttgacgggag cgtcctgcag ttcggtcaga cggtgacgaa ccgggggcag acgggcggca
     1681 acctgtacgc gttcggacag acgatcacga cgcccggcgt ggcgcgggtc ggcgggaacg
     1741 taaccgtatt ctccgaaacg gcgaccatcg aaggttccgt gggacacgat gtgacgagct
     1801 ttggccgcca actggatctc ggcggcagcg tcggccggcg cgtgaacgcc tacggcgacc
     1861 gcgtcgacat ccggtccagc gccaggatcg acggcggcct gaccgcgcac gtcgcgaacc
     1921 gcgagaacgt gcgcgtcgat ccaggcgcca ccgtcggcgg cgagacgcga atcgacgtga
     1981 cggaaccttc gcccagccgg ttcgcgacgt cgagcttcta cgtccgccag gtcatccgcc
     2041 tcgctgcggc tttcattgcc ggatggatct tcttccggct cgtgccccgt gccgcggcga
     2101 tcacgctcgg caccggaggg gccgcgctca cggcccttgg catcggcgcc gtcgccgcct
     2161 gcgcgacgcc ggttctggcc atcgtcgtcg gcatcacgat cgtcggattg ccgatcgcgg
     2221 tctttgcggt cctgctctgg atcgttcttc tctatctcgc caagatcgtg attgccctgc
     2281 tggtggggcg ggcgctcgtt ggtgccagcg cccggggcat cgcgacggcg cttctggccg
     2341 ggctggtgct cgtcatcgtg gccgtgaacc tgccgtatgt cggcggcctc gtcagcctgc
     2401 tcttgaccgt caccgggctc ggcgtgctcg tcgtggaagc ctcgaggtgg tacaggggat
     2461 caggcggcgt tccggttcgt gcgtgagccc tggtcgcgct cgcgtctcgc ggcgcgccgc
     2521 ccgacgatca cgcggtagta cacgttcagg cacgcgagag caaacacggc tgtcggcagg
     2581 agcgcgctca gcccgacgag cgcccagctc tggggcggcc gcggcttgaa ttcgtacttg
     2641 acgacttcgc ctggcccggc gtcgagccgg ctcgacagca tgcagagccg atccgagtaa
     2701 tcagggtact tctcgaggac cacctgttcg agttcctcgt ccgaccagtc gtcgtagtag
     2761 ccgggatact cgtatcggac cagcgtggcg aggcgcggcg agcagaaccc ctgccgcagc
     2821 tcggcttcgt cgatcggcct gaccgggtcc gtcggggttc ccgtcaacgg aagcgagccg
     2881 acccggccgg ccgatgcgag aaacgatgga atcgcgttct tcacgttcgg gtccacactg
     2941 cgcgtgaagt cctgcagcga cacgatcaca tcgacggtac cgcgcacggg ctggcggtgc
     3001 gtgtgtacga agcgggccgt gaagccgaat accgccacga cagcgacgac gaagagaccc
     3061 ttgtatgtcc cgcgccaccc ggcgctcaga tcggtatcga tctcgtaccc gatgagagcg
     3121 gtcaggaggc cgcggcgttc acgcggctga cgaggcgccg acggcgcctg actgcctgcg
     3181 tgagccggag tggagtcgaa cgtatagcca agcgcctcga ggcggcgccg ctcgctgccg
     3241 cagcggcaga cctcgacgtt ccgcggcaca tggcgtgcac aggatgggca ctgccaggca
     3301 tttccctgca ccctattctt atcggcccca cggccggcgt cgcttagcgc gcgtccagtt
     3361 cgaggacgag gcccaccacc gtgtcgtgga tgagcgtcat gccctggggc gtgagcttgt
     3421 ccgccgtgtc ctccggcgtg tgaatggtcc ggagaatcga cggcgcgaac cccggagtca
     3481 gccccgattc cttgccgccg ttgagcagca gccagatctg atgggcctcg agggcgggaa
     3541 ggacggccag cgagatggcc gggatgcccg cggcctggaa actgcggtcg tcgctattcg
     3601 ggagagacgg aaacgccatg caggcgtagt tgcgggcggc gcataccctg gccagcgagt
     3661 gccgaaggct ctcggctccc ggcgctgcgc cgctgaggat cgtgtcgccg tagccggcga
     3721 tgtccacgtt gaccatcgcc gccacgccgg ccttgtcgag caggctcgcg aagtgcctcg
     3781 acccggcaag gccgatctct tccatgtcga agaacacgat ccggacccgg tgcctcagcc
     3841 ggcgcgcccg cagggcctcg gccgcgcgca ccaggacgac gacgccggcg ccgttgtcca
     3901 ccatgccgtg gctgtagccg cccgctttga gcgccgccgc gtcgaggtgc gcgcccacga
     3961 tgatctccgg cccggccggt cccggcagat ccacgatcac gttggttccc tgctccctgg
     4021 gatcgcgctg acgcgacgtg ttggggaatg tctggagcgt gaaggggagg ccccgccggc
     4081 gcagcaacga ctgcacggcg tcgacgcgcg cggcgttgga cggttgatcg agcgtccgtg
     4141 catcagccac cacgtcggag gcgagcggcg cggtgggcac gacggctggc gcgggcatgt
     4201 ttgcgcaacc ggcggcgagc acggctgcga ccgaggtgag cacaacgaga cgacgtcgag
     4261 gcatggcggc gagattacta cggcgtgaat gcctcacgcc ggcgggatct tgtccagaaa
     4321 attccgggtc cgctctgcca gggtcgtcag ccacgcgatc tgcagttgcg tgcccgtcct
     4381 gatgagatcc acaagcgggg gaggcagcac cgaccagtcg atggcgaggc gtccccagtc
     4441 aggccgacgg ccatcgtaat cggtgaaccc gtagcggcgc gcaacctccc acgagctgaa
     4501 gagctgcccc gtctgcgtca ggatgtcggg atcgccggcg agggcggcca ccgcccgccc
     4561 gacgaacagc ggcgactccg attccagaaa attcggatcc tgcttgccgg cgtcgcgcca
     4621 gttcgcttcc gtgaccccgt ggtgttccag catggactcc gaccggagga atcccggcgt
     4681 gatcgcgagg gccgagacgc cgtgttcctt gagctcggcg gccatcgtca gcgcgagcac
     4741 gcggagcgcg accttgaccg attgcgacac cgggttgccg ccggcggtca gcaaatcgtt
     4801 ctccgtcacc tgcacgatca gtccgcggcg atgacgcatc atgaacggcg cggcgtgctt
     4861 tgccgtgatg atgtgggagg tcagcgcctg ccggaagccc gcgtcggcgt ccttcagatc
     4921 gacctgccag aagtttccgt attgccccat catcggatct tcaccggcga cgctgtcggc
     4981 cacgatgtcg agccgctggt gctcccgatc gatgcgcgcg aagagcgcct ccacctccgc
     5041 ttcgatcgtg tggtccacgc ggacggggat ggccgtgccc ccggcgtcgt tgatcatctc
     5101 cgcggtctcg tcgatcgtct cgggccgttt gtaaggggag ggctgcccct tcacgcttcg
     5161 gccggtgcag tacaccgtgg cgccggcttc gccgagcgcc cgcgcgatgc cgcgaccggc
     5221 gccccgcgtg gcgccggcga cgacggcgac gtgtccggtc aacggcttcg gtggcatgac
     5281 gcgcactata gttcaccggt cttgggcgaa accttccagt cgacgcgaac ggagcgggta
     5341 gcctcgatca tcggagccag gaacgtgctc ttggcctgct cgaacgctgc cgtcagcacc
     5401 gcatgatcgg ggggcgcccc atcgatgtcg gtaaacccgt actcctctga cagggcgtgg
     5461 gccgtgtaga tgccgccgtt cttccgcgca acgtccgggt cggcggcgag cgcggcgacc
     5521 gcgcgtccga cgaagcaggg cgtttcggat ccgccccatc ccatcgccgc cgcctgcgga
     5581 ctcttcaccg cgtcgcgcca gttcgcctcc gtgacgccga agccgtcgag gatggcctcg
     5641 gtccgcatga agccgggacc tatggcgacg gccgtcacgc cctgctttcc caaatcgttg
     5701 gccaggccga acaccagccg cttgagcagc gtttccatga cgtcgaaata aaacgcgccg
     5761 tgatagccga cgttgtcgtg ctcgaccagc tcgacgatga ggcccgaccg gcgtttcacc
     5821 atcatcttgg ccgcgtgcca ggaggtgacg atgtgcggcc agatccagct ctcgacgaag
     5881 gcccggcccg agtcggggct ctcgttcagg aagttcttcc acgaggcagg ctgcccggtg
     5941 atcaccgtgg cgagcacgtc cagacgcttc cgctcgcggc gtacccgggc gaacagcgcc
     6001 gccacctctt caccgacctc atgatcgacg cgcacgggaa tgcccgttcc gccggccgcc
     6061 gtcaccatct cggccgtctc ctcgatcgtc tccgggcgac gggcgtgcac gtggttcgac
     6121 gtatttggct gtgtgcggct gctgcgcccc gtgcagtaca cggtggctcc ggcctccccg
     6181 agcatgcggg cgatgcctcg tcccgcgccg cgcgcggcgc cggcgacgac ggcgacgtgt
     6241 ccggtcaacg gcttcgtcat gacggcgcac tataaatgaa cagacattta caaatcaagc
     6301 agaaactaaa cggccgttta taatggctgc acccgatgag cccgcgcccc cggtccacca
     6361 gcgacgccga gatcctctcg gcgacccatc gcgccgtcac ccgcgtcgga ccgaagcgcc
     6421 tcacgctcgc cgacgtcgcg cgcgaagcgg gcgtctcgcc ggccaccctg gttcagcgct
     6481 tcggctccaa gcggaagctg ctgctcgcgt tcgtcgccac cggcagcagc gagatcgagg
     6541 ccgagtgggc tcgcattctg tctttggcgg agcggtcgcc cctcgaggcg ctctacgcct
     6601 acgcggagtg catggccggc ctggcgccca accccgacgt gctggccaac catatgtcct
     6661 tcctgcagat ggatctcgtc gatccggagt ttcaccacca cgcgctcgcg caggcgcgcc
     6721 acacgctgga gtttctccgc aagacgctcg acgacgccgt cgacagaggg gagctggcgc
     6781 cgtgcgacac gacgcgcctg gcgcgcgccg tccaggccac catcggcggc tcgctcatgc
     6841 agtgggcgat cgatcgggaa gggaagctcc gtcggcgcct ccgggaggat ctcgagacgc
     6901 tgttgcagcc gcgatatccg cggcgcgcga agcgcccgca cgcccggcgg cgccgctgac
     6961 gccacatccg gcacaccgag gcacgagcct cggtgcgtcg tgatcgtcta gagaagcgaa
     7021 ctcacctgca ggtcacagtt ctctgatcgg ccggttcgtc gccaggaccc acgccgccac
     7081 cgcgctgagc gtcagcccgc tcaccgtcag gacggccggc gcgctcgtga gcgtcgcgag
     7141 ccagcccccg gccaggctgc cgagcggcat gccgccgcgg aacgccacga ggtagatgct
     7201 catcacgcgc ccgcgcatct ggttcggcgc gttgagctgc acgagcgagg acagcgtggc
     7261 gaacgccatc accatgcacg cgcccgccgc gaagaggagc gcggcgttca gccagaagat
     7321 ccgcgtgacg gcgaacagcg ccacgaacgc gccgaaggcg atctgaatca gcaggcacgt
     7381 ccgtcccatg tgcggaaacc ggccgcgcca ggccacgacg agcgcaccgg tgacggcgcc
     7441 ggcgccggcc gacgacatca ggtgggtgta ctgcgcgact ccgccgccga gcacgtcctg
     7501 cgcgaagagc ggcaggaacg tcagcaccgg actgcccagg aaggtcgcgg caaaccccag
     7561 cagggcgagc ccgacgagcg ccgggtgcga tccgacatac gacagaccgc tcttgaactc
     7621 ctcgtgcagg cgcgtggtgg acgcgggcgg cgtgtggcgg acgtgcaggg acaggatcgc
     7681 ggcaatgacg gcgacaaacg acaggccgtt gaggccgaag caggccacca tgccgaacgc
     7741 cgcgagcgcc gcgccggcca gcagcggtcc gatcacgcgc gccaggttga actggatcga
     7801 gttgaacgcg atcgcgttcg gcacgtggtc cttgctgacg agcgacggca gcagcgactg
     7861 atacgcaggt ccgccaaagg cctgcgccag gccggtgacg accgacagcg cgagcacgtg
     7921 ccagatgcgg acgagatccc agtagacaag cgcggccagt gtgaacgcgg ctcccatctg
     7981 cacgcactgc gacgccagca ggagccgtcg tcgatcgtgc cggtccgcga tgacgccgcc
     8041 gacgagcgtg aagagcagga tgggcagctc ccccaggaat gaatcgaggc ccaggaagaa
     8101 ggccgaggag gtgccggtga tcgtcagcac gagccagttc tgggcgacct tctgcaccca
     8161 cgtgccgatg gtcgacagga acgctccgca ccagaggatg cggaaatcgc ggtggccgag
     8221 cgcagcgccg agccgcgcca gcccgcgcga ggcgggcggg cccggccccg ctaccgttcc
     8281 cgcagtcacc gattcgtcgg atgagggagt ggacgcctgg gttcgagaac gatcaaacac
     8341 cgaacgagga attgtacact ctggccgcga tgcgtccctt ggcgccgcgc gctctgggcg
     8401 gccttgtgct cctgtgggcc ctggtgtcgg tcgtctccgc atggcaggcg gccgccgcgc
     8461 gcggtccaga tcgagtgacg gcgctcgagg cggggttccg cgcgctggcg gcgtcggcgc
     8521 ccgcctccgg cgacatcggc ttcctgaggc actacgagca ccccgacagc ccggacgaca
     8581 tccgcgatta ctacgtggca cagtatgcgt tcgcgcctcg cctcgtcgct cgtcgaaccg
     8641 acgtggatct tctcatcgtg gctcagggcg ccgcacatcc ggatcgggac gagcgtctcg
     8701 ccgggtttgc gctcgttgcc agctcccctg aaggtcatcg cttgtatcga aagctgatca
     8761 ggtgatccgc gaggcgatcg cactgctcgt tcccgcctcc gcgatcgtgc tgttcgcgcc
     8821 gtcgctccgc agcgcctgcg gccgatcccg tgccgcggcg gccgcgctgg ccctgtgcct
     8881 cggcatcggg gtgtcttcgg tcacgacggc cggtctcatt gcctcgggcc tgacgccgcg
     8941 tgacggaatc ttcgtcgtga ccgacgtcgt gatctgggcg atcgtcggca cgaccggctg
     9001 gtggctctgg cgccggaacg cccggtcggt gtcgcacacg catccgccgg tccacgcgcg
     9061 atcgttgaac ggtctcgact ggatcgtccg cctcgtgttt gctgcggttg cggcgatcgc
     9121 gctcggagtc gtcgcgacgt ctttccgggt tgcgccccat ggggactggg acgcctgggc
     9181 tatctggaat ctgcacgcgc ggtttctctt cagcggcggc gaccgatggc gcgacctgct
     9241 gatggtcgat tggtcgcagc ccggctatcc gctgctgctg ccggcgtcgg tggctcgcgt
     9301 gtgggcctac gccggacacg acacgacggc aggtccggcg atagtcgcga tcgtcttcgg
     9361 ggttaccacc gtgaccatcg tcatgtcgac gatcggtctc gagcgtcccc gggcctggat
     9421 tgccggggcg ctgctcgccg gcgccggatc gtttctcgtc cagattccgt cgctgtgtgc
     9481 cgatgttccg atcgcctgct tcatggtggt gtcactggcg gcggcgtgtg gacgtgaggg
     9541 tgtactggcc gggcgggaga cctcgcgcac ggcggcgctg gtgtccggcg gggcgggagc
     9601 catggcggca tggacgaaga acgaagggct cgtcttcgtt ctggtgatgt tgctgcttgt
     9661 cgccgttgtg accgtgcgcc gtggcggggg gcgatggctc tcgtggtgga tcgctggcgg
     9721 tgcgccgatg ctcgccgcac tcgcgtggtt caagctcgcc ctggcgcctc cttcctcgtg
     9781 gttcgcacag tccccgctgg tctatgcggg tctgttgtcc gacacggagc gttatgccgt
     9841 gacggcgtcg ttgatggccc aacacctctg gcgctggggt gcgccgcttg ccggcatcat
     9901 tccgcttctg gcgctggccg cagtctggct gtccgtcgtg ggtggcgggg ccatgcggat
     9961 gatggccgcc ctcgtcgcgc tgatgttcgt cggttattgc ctcgcctact tgaccacgcc
    10021 attcgacatc acatggcatg tcacgacgtc gttcgatcgg cttctggtgc aggtgtggcc
    10081 gtcgctcgtc ctcacggtat ttttcgacga gacgcgtctt aaccgcttgg aatacgcctg
    10141 aattcatcga tattcgcctc ttgatcgcct aaggaagttc gcatatagtg ttggcgaaaa
    10201 tagagcgaat gtcatgacca accctgctgt tgcggtcctt caatccgccc tgcgtgcgcg
    10261 caagctcgat cgcacgctga cagacgcgct gccgacgctc gagcgagcgg atccatcgtc
    10321 gctggcgccc atggatgtag cgtccatcga cgcctgcctg cacgggggcc ttcctcgagg
    10381 gcagctgtcg gagttgtcgg ggccctgctc gtcgggccgg acgacgctgc ttctgcaatt
    10441 catggcagcg gccacgcggc ggggggagat tgccgcgtat gtcgatacct tcgatcggct
    10501 ggatgtggcg tctgccgcgg cagccggcgt ggatctcgat cggctgttgt ggatccgcgg
    10561 ccagtcagtg atcggggatc acaaaccacg gagtcacgga ggcgcggagg aaaacaatca
    10621 agaatctccg tgtctccgcg tctccgtggt tcgtgatcgt gctcttgacc gcgcgctgaa
    10681 ggcgcttaat ctggtcctgc aggcgggcgg gttcggcatc gtcgccatcg atctggcgga
    10741 cgcgccgccc cgcgagctca ggcagattcc gttcacgaca tggccgcgcg tgcagcgcgc
    10801 gatcgaaggg agggacacgg cat
//
LOCUS       DBNGSH010000825         2495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4681635, whole genome shotgun sequence.
ACCESSION   DBNGSH010000825 DBNGSH010000000
VERSION     DBNGSH010000825.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4681635"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2232
                     /gene="fdnG"
                     /locus_tag="AC16CY_18965"
     CDS             <1..2232
                     /gene="fdnG"
                     /locus_tag="AC16CY_18965"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HIR3355940.1"
                     /translation="LGGLIHYALSRSRFHEEYVRLYTNAPLLVSETYAFDETRGVFSG
                     WDEERKAYTDHSSWSYELGPDGFARIDPTMQHPRSVFQVMKRFYSRYTPEAVSNICGC
                     SVEDFDAVAELVTSTYTPDRAGTIMYALGWTHHSHSVQLIHAAAMLQMLLGNIGRPGG
                     GLNALRGHANIQGGTDCGMAYHNVPGYIPIPKAGHDSLDTFLRAVTPKPLRPNSTNFW
                     VNTDRFFVSQLKAYYGNAATKANDFGYGFHPRLPQSEQGTDENWSWAYMFDHMYRDQI
                     EGFFSFGMNPVSNGPHSRKAIAALAKLKWLVVAENFEQETATFWREDILALVEKKPED
                     VQTEVFLLPAANFAEKDGTFVNSARWIQWKWKAVDPPGEAKPDQEIIARIFLKVRELY
                     ERDGGTYPEPVRSLNWWYTNPTSPSLDEVCREINGWALEDVKDPAGAVVKRKGQQLSA
                     FLETRADGTTLSGNWLYIGMYTDQGNLTQRRSYADPSGLGRYPEWTFSWPANRRVLYN
                     RCSADAQGRPWDATRVGIAWNGERWVGDVPDYTATSAPAASLGPFIMLPEGVGRFFVP
                     GQFADGPWSEHYEPVESPVANPLHPPTSSNPAVQRFTTEFDVLGTAEEFPIVCTTYRL
                     TEHFHYWTKNNPYSVQLQPEFFIEIPQELASQHGVSSGDTVRVTSARGSLEGKAMVTR
                     RIKPMQVGGRTVYQIGFPIHWGFLGRGQQRGSLANLVTPTIVDPNSFAPEYKGFLVKI
                     EKV"
     gene            2294..>2495
                     /locus_tag="AC16CY_18970"
     CDS             2294..>2495
                     /locus_tag="AC16CY_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3355941.1"
                     /translation="MSLEIVRSSAASSPAPGVRVQPTVTKLVDTSTCIGCKACEVACQ
                     EWNDLPPEATVQSGTYQTLPATT"
BASE COUNT          457 a          813 c          812 g          413 t
ORIGIN      
        1 ctcggcgggc tgatccacta cgccctcagc aggagccgct ttcacgagga gtacgtccgg
       61 ctgtacacga atgcgccgct gctcgtcagc gagacgtacg ccttcgacga gacgcgcggc
      121 gtgttctcag ggtgggacga agagcggaag gcatacaccg accattcgag ctggagctac
      181 gagctcggcc cggacggatt cgcgcggatc gatccgacga tgcagcaccc gcggtccgtg
      241 ttccaggtga tgaagcgctt ctactcgcgc tacacgccgg aggccgtgtc caacatctgc
      301 ggctgcagcg tcgaggactt cgacgccgtc gccgagctcg tgacctcgac ctacacgccc
      361 gatcgcgcgg gcacgatcat gtacgcgctc ggctggaccc atcacagcca ctcggtgcag
      421 ctcattcacg cggcggcgat gctccagatg ctgctcggca acatcggccg cccgggcggc
      481 gggttgaacg cgctgcgcgg ccacgccaac atccagggcg gaaccgactg cgggatggcg
      541 taccacaacg tccccgggta catcccgatc ccgaaggccg gccacgactc gctcgacacg
      601 ttccttcgcg cggtgacgcc gaagccgctg cgcccgaact ccaccaactt ctgggtcaac
      661 accgatcgct tcttcgtcag ccagctcaag gcgtactacg ggaacgccgc caccaaggcg
      721 aacgatttcg ggtacggctt tcacccgcga ttgccccagt ccgagcaggg gaccgacgag
      781 aactggagct gggcgtacat gttcgaccac atgtaccgcg accagataga aggctttttc
      841 agcttcggca tgaatccggt cagcaacggg ccgcactcgc gcaaggcgat cgcggccctc
      901 gcgaagctga aatggctggt cgtcgccgag aacttcgaac aggagaccgc cacgttctgg
      961 cgcgaagaca tcctggcgct cgtcgagaag aaacccgaag acgtgcagac ggaggtcttc
     1021 ctcctgccgg cggccaactt cgccgagaag gacggcacct tcgtcaactc ggcccggtgg
     1081 attcagtgga agtggaaggc ggtcgatccg ccgggagagg ccaagccgga tcaggagatc
     1141 atcgcgcgca tcttcctgaa agtgcgggag ctctacgagc gcgacggcgg cacctacccg
     1201 gagccggtgc gcagtctcaa ctggtggtac acgaacccga cgtcgccttc gctggacgag
     1261 gtgtgcaggg agatcaacgg ctgggcgctc gaggacgtga aagatcccgc cggcgccgtc
     1321 gtcaagcgaa agggtcaaca gctctcggcg ttcctcgaga cgcgcgccga cgggacgacg
     1381 ttgtcgggga actggctgta catcgggatg tacaccgacc agggaaacct gacgcagcgc
     1441 cgatcgtacg cggacccgag cggcctcggc cgctatccgg agtggacctt cagctggccc
     1501 gccaaccgaa gagtgctgta caaccgctgc tcggccgacg cgcaagggcg gccgtgggat
     1561 gccacccggg tgggaatcgc ctggaacggc gagcgatggg tcggcgacgt tccggactac
     1621 acggccacga gcgcgccagc tgccagcctc ggtcctttca tcatgctgcc cgaaggcgtg
     1681 ggccgattct ttgtcccggg acagtttgcc gacggaccct ggtcggagca ctacgagccg
     1741 gtagagtcac ccgtcgcgaa cccgctgcac ccgccgacca gctcgaaccc ggccgtgcag
     1801 cgcttcacga ccgagttcga cgtgctcggg acggccgagg agttcccgat cgtctgcacc
     1861 acgtaccgcc tgaccgagca cttccactac tggacgaaga acaatccgta cagcgtgcag
     1921 ctgcagccgg agttcttcat cgagattccg caggagcttg cgagccagca cggcgtctcg
     1981 agcggcgaca ccgtgcgcgt gacgtcggcc cggggctcgc tcgagggcaa ggcgatggtg
     2041 acgcgccgca tcaagccgat gcaggtcggg ggccgcacgg tgtatcagat cggcttcccg
     2101 attcactggg gcttcctggg gcgtggtcag cagcgcgggt cgctcgccaa tctcgtgacg
     2161 ccgacgatcg tcgatccgaa ttcgtttgcg cctgagtaca agggtttcct ggtaaagatt
     2221 gagaaggtgt gatgtcgttc acacttctcg cttctctctt ctcggttcgt gttcagtttc
     2281 aggttcgtgt tcgatgtctt tagagatcgt tcgaagttcg gcggcctcgt cccctgctcc
     2341 cggcgtgcga gtgcagccga cggtgacgaa gctcgtggac acgtccacct gcatcggctg
     2401 caaggcgtgc gaggtggcgt gccaggagtg gaacgatctg ccgcccgagg cgacggtgca
     2461 gtccggcacg taccagacgc tgccggccac gaccg
//
LOCUS       DBNGSH010000826         8514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2981865, whole genome shotgun sequence.
ACCESSION   DBNGSH010000826 DBNGSH010000000
VERSION     DBNGSH010000826.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2981865"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="AC16CY_18975"
     CDS             <1..152
                     /locus_tag="AC16CY_18975"
                     /inference="COORDINATES: protein motif:HMM:NF026032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR3355942.1"
                     /translation="SAPEGVAQLLARHPDVSIFTPVVDRELNARKFIVPGLGDFGDRL
                     YGTEG"
     gene            365..439
                     /locus_tag="AC16CY_18980"
     tRNA            365..439
                     /locus_tag="AC16CY_18980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Val,seq:cac)
     gene            complement(545..1987)
                     /locus_tag="AC16CY_18985"
     CDS             complement(545..1987)
                     /locus_tag="AC16CY_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HIR3355943.1"
                     /translation="MSTHTRSLAVGVFVAGTGVLVSAQAITGPSSSASPYVLRAEPGV
                     VSAAILTVGDAVGGYRLVGIPDGLGAFDNGDGTFTLLVNHEISAALGVPRAHGAKGAF
                     VSRWTIRKGDLAVLHGSDLIQEVATWNVATGSWNAPATGVAMSRLCSADLPPISALHD
                     AASGLGYAGRMFLNGEEVSPGQEGRAFAHLMDGTSYELPALGKASWENLLARPGTGTR
                     TVVAGLDDSSGSVAGQVYVYVGEKVGSANPIEAAGLSHGQLFGIQVDGVAFETAGTQI
                     PDGTRFTLYPFGNVSGTTGAAIESMSVANGVTAFLRPEDGAWDPSNLNDFYFVTTATY
                     TGPSRLWRLRFDDGAVPELGGTITLILDGTEGHHMFDNLTITRRGRILLQEDPGVPGV
                     APDHLALIWRYTIETDALTAVARHDPERFILGSPGFLTRDEESSGIIDVSDILGAGWF
                     LLTVQAPYNIGDPELVAGGQLVALHVPPGR"
     gene            complement(2205..2738)
                     /locus_tag="AC16CY_18990"
     CDS             complement(2205..2738)
                     /locus_tag="AC16CY_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355944.1"
                     /translation="MTAARGVGLVVPVYLLLASPALVAQESKSAAVVRELTQALDQLK
                     LDAIAAKHGPERYVAALYIAGSQLLVIAAKYSVPARLDSLIAKREYQEVYADLSSASE
                     QASKMFVMDLGANGLRFKRATNEPFDTVDTATGSVVFNGERGKLSEDDYKKVFTTNDE
                     QYASMIQALVAELKKPS"
     gene            complement(2805..3266)
                     /locus_tag="AC16CY_18995"
     CDS             complement(2805..3266)
                     /locus_tag="AC16CY_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355945.1"
                     /translation="MNLTEFFLGLIAVAVLAMAVVQVWAAVVAAQAARRLGETVSKLE
                     QDVRPIVANVQAMSADAARAAAIATAQVERAERLLNDASRRVEETMAVVQQTILGPAR
                     DGMAIFQAVKTIFTAFSDLRARSRRQPPASGRVPVAVPDPGADDDSASFIG"
     gene            complement(3263..3535)
                     /locus_tag="AC16CY_19000"
     CDS             complement(3263..3535)
                     /locus_tag="AC16CY_19000"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR3355946.1"
                     /translation="MSQQGSGGGMFLAFVAGAIAGAAVALLFAPATGEETREFLGQRA
                     REGRDRAAEAARQSREMISKQRENLTTAFDRAREQFQATRETGQDA"
     gene            complement(3562..4602)
                     /locus_tag="AC16CY_19005"
     CDS             complement(3562..4602)
                     /locus_tag="AC16CY_19005"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR3355947.1"
                     /translation="MPRTRSLAWAELKIGVVSVLAVILSVIMIFMIGGEGGFFWQRVG
                     LKTVFDSIPGLKEGAPVRLAGVEIGSVTGIAFTGDRVEVLMEVGRGNQSRITTASIAT
                     LGSVSLLGEAALDITASSTGQPIPEWGYVPSGRAMGTIGDVATQAGVAMEEATALVSS
                     IRAGRGTMGQLMTDDRLYQELTALVSSAEDVASGINKGRGTLGRLASDPAAAKALEAS
                     LDNLQAVTARIRAGEGSLGQLLSNDALSRSLTSTTANLDAITGRINRGEGTAGKLVTD
                     AQLYDRFNSLSDRLDKLVGGLQQGEGTAGQLLRDKKLYDNITETMAELRGLVIDIRRD
                     PKKYLNVKVSLF"
     gene            complement(4613..5458)
                     /locus_tag="AC16CY_19010"
     CDS             complement(4613..5458)
                     /locus_tag="AC16CY_19010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355948.1"
                     /translation="MDRQPTEDRQPTGVQLVESPAIELARVSLSFDESVVLQDVSFTL
                     LPGHTKIILGASGSGKSTILKLILGFWKPDGGIIRVRGERTDTMSERDLMRVRADIGM
                     VFQEGALFDSLSVAENVGYKLYEESGTPPGDVRRRVEQVLGFIGLADYIDNMPSELSG
                     GQRRRVAIARAMAAKPGIILYDEATTGLDPITAITVDEEIIKLRDLERVTSIIVTHQL
                     RDAFYIATHEAIRDGSRVRIVAADEQKADEAEFIMLRNGRVAFEGNASELRATHDSYL
                     KTFLS"
     gene            complement(5481..6254)
                     /locus_tag="AC16CY_19015"
     CDS             complement(5481..6254)
                     /locus_tag="AC16CY_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR3355949.1"
                     /translation="MLDILTTWLKGGVLEVQEYVRLQAAALQAAVSRPFYARDVVEQF
                     DQIGIGSLTVVVLTGFFTGAVLALQTGITLDNFGARPFVGQLVSASMIRELGPVLTGL
                     MLAGRIGSGIAAELGSMVVTDQINALRALGTDPVRKLVVPRVLAGLFMAPVLTVLADA
                     LGILGGWFIAVFQLRVASSLYWTSIVDVLALPDAWMGLIKPFCLGYVITTIGCHVGLR
                     TKGGTQGVGRSTTNAVVASSVAVIAVDFFLTRLLITLLY"
     gene            complement(6254..8269)
                     /gene="uvrB"
                     /locus_tag="AC16CY_19020"
     CDS             complement(6254..8269)
                     /gene="uvrB"
                     /locus_tag="AC16CY_19020"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR3355950.1"
                     /translation="MPSVHDRFRLVSDFEVRGDQVHAVPELVEGLNRGDTHQVLLGVT
                     GSGKTFTMAQVLAQVHRPTLVMAHNKTLAAQLFQEFRRFFPENAVEYFVSYYDYYQPE
                     AYVPATDSYIEKEATINDEIDRMRLSATRSLFERRDVIIVASVSCIYGLGSPEAYYGL
                     LLPLELGQRIERDQILRKLVEIQYERNDTEFGRGTFRVRGDIVEVVPSYEEHALRIGL
                     FGDEVDELAWFDPLTGKVIRRLDKVAVYPKSHFVTSRDRTKQAVETIKAELAERRSLL
                     ESEGKLLEAQRLHQRTMFDLEMIREIGYCHGIENYARHLTGRPPGAPPPTLLDYLPDD
                     ALVIVDESHQTVPQIRGMYQGDRSRKEVLVAYGFRLPSALDNRPLNFEEWEQRVKQVV
                     FVSATPGPYELSRSLGVVVEQVIRPTGLMDPPIEVRPVRGQVDDLLGEIRDRAARKER
                     VLVTTLTKRMAEDLTTYYQELGVKVRYLHSDIDTLERVEILRDLRRGAFDVLVGINLL
                     REGLDLPEVSLVAILDADKEGFLRSAGSLIQTSGRAARNVNGRVIMYADTMTASMRLA
                     IAETERRRALQGAYNEANGITPESVVRQIDDVMASVYERDYMTPAETPQETFRTQAEL
                     DAEITRLEKDMRAAAANLDFERAAALRDRLKALRTRDLGFSGITAGR"
     gene            complement(8305..>8514)
                     /locus_tag="AC16CY_19025"
     CDS             complement(8305..>8514)
                     /locus_tag="AC16CY_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355951.1"
                     /translation="FFPSSVKQVDEAYDTIVAQVQAQYTLGYVSTNTAADGRWRKVEI
                     RVPRSVAKGTRIQARRGYFAPYRPN"
BASE COUNT         1472 a         2862 c         2687 g         1493 t
ORIGIN      
        1 tctcggcgcc cgagggagtc gcccaactgc tcgcgcggca ccccgacgtc tccattttca
       61 cgcccgtcgt cgatcgcgag ctcaacgcgc gcaagttcat cgttcccggg ctcggcgact
      121 tcggcgaccg gctgtacggg accgagggct agcttcgcgg tccttgggcc ttggttcttg
      181 gtccttggtc cgtacttagc tctttgtccg ttcttggtcc ttggtacttg gtccgtcgtg
      241 aacacgtctg accaggacgg atcacggact cacgaccaag gacggaccgg ggaccaaagg
      301 acggaccaag cgccaagaac taaggaccaa aggaccatgt tcgggttaga ctcagattct
      361 ggtcgggcgc gtggctcagc ggtagagcat tcgcttcaca cgcgaggggt cggtggttcg
      421 attccacccg cgcccaccaa ccttcgctca cgcttgttaa cgagagcgtg agctacggtt
      481 ggcgagcgca accttcacgc gaaggtggac cgcgaagggc gtttacacca cgacagccga
      541 agccctaccg cccgggcggc acgtgcaacg ccaccagctg gccgcctgcc acgagctcgg
      601 gatcgccgat gttgtatggc gcctgaacgg taagcaggaa ccagcccgct ccaagaatgt
      661 cgctgacgtc aatgatgccg gacgactcct cgtcacgggt caggaaccct ggtgaaccca
      721 ggatgaagcg ctcggggtcg tgtctcgcga ccgccgtcag agcatcggtc tcgatcgtgt
      781 agcgccaaat caatgccaga tggtcgggag cgacccccgg aactcccgga tcttcctgga
      841 ggagaatccg gccgcggcgg gtgatcgtca ggttgtcgaa catgtggtgg ccctccgtac
      901 catccaggat cagcgtgatc gtgccgccga gctccgggac cgcgccgtcg tcgaagcgca
      961 ggcgccaaag ccggctcgga cccgtgtagg tcgctgtcgt cacgaagtag aagtcgttca
     1021 ggttggatgg atcccaggcg ccatcctccg gacgcagaaa cgcagtgacc ccattggcca
     1081 cgctcattga ttcgatcgcg gcgcccgtcg tgccggagac attgccgaat gggtacagcg
     1141 tgaaacgcgt gccgtcggga atctgcgtgc cggcggtctc aaacgcgact ccgtcaacct
     1201 gaatgccaaa cagctggcca tgcgacagcc ccgccgcctc gatgggattc gccgaaccca
     1261 ctttctcgcc gacatagacg tacacctgtc cggcaaccga ccccgagctg tcgtcgagac
     1321 cggcgacaac cgttcgcgtt ccggtacccg gccgtgccag caggttctcc caggacgcct
     1381 ttccgagggc cggcagctcg tagctggttc cgtccatcag gtgcgcgaag gcgcggcctt
     1441 cctggcctgg actcacctct tcgccattga ggaacatccg acccgcgtat ccgagtccgc
     1501 tcgcggcatc atgcagggcc gagatcggcg gcagatcagc ggaacagagg cgtgacatcg
     1561 ccacgccagt cgcgggggca ttccatgacc cggtcgccac attccaggtg gccacctcct
     1621 gaatcaggtc actcccgtgg agcacggcga gatcgccttt gcggatggtc catctggaca
     1681 cgaaagcgcc tttcgcgccg tgtgcccgcg ggacccccaa ggctgctgag atctcgtggt
     1741 tcaccagcaa ggtaaacgtc ccgtcgccgt tgtcgaacgc gccgagcccg tcggggatgc
     1801 caacgagacg gtacccgccg accgcatcgc cgaccgtgag aatcgccgcg cttaccacac
     1861 cgggctccgc acgaaggacg tacggagacg ccgagctgct cggaccggtg atcgcttgcg
     1921 ccgacaccag gacgccggtg cccgcaacaa acacaccgac tgccaacgac cgagtatgtg
     1981 tagacatggg ccgctccttt caattgtcaa gcgtgtgagt gccgcgcggc tgttctgtga
     2041 ctgagtgcgt taacggaggg agaactggct cacatgcagg gacgggaggc caggccggag
     2101 ttcgagggat ggcgcgagcg acggttggca aggcccagat atgcaaagag ggcagtccga
     2161 agactgccct ccctgttgcc tcgagcgttg gcgcagagcg ccggctacga gggcttcttg
     2221 agctccgcca ccagcgcctg gatcatcgac gcgtactgct cgtcgttcgt cgtgaacacc
     2281 ttcttgtagt cgtcctcaga aagcttgccc cgctcgccgt tgaagaccac actgcccgtc
     2341 gcggtgtcga ccgtgtcgaa gggctcgttc gtcgcgcgct tgaatctcag gccgttggca
     2401 ccgaggtcca tgacgaacat cttcgacgcc tgttccgacg cgctggacag atcggcgtag
     2461 acctcttggt actctctctt ggctatgagc gagtcgagcc gcgccggcac cgagtacttg
     2521 gccgcaatga ccagaagctg actgccggcg atgtagagtg ccgcaacata cctttccggc
     2581 ccgtgcttgg cggcgatcgc atccagcttc agctggtcga gcgcctgcgt cagctcgcgc
     2641 acgacagccg ccgatttgga ttcctgagcg acgagcgctg gcgaagcgag cagcaggtac
     2701 accggaacca caaggcccac gccgcgagca gcagtcatca tggtgtcccc ccgctggaaa
     2761 gctgcgcgag tttacccacg cgacgccacg ccggtcaacg aagatcagcc gatgaacgac
     2821 gccgaatcgt cgtcggcgcc cgggtcggga acggccaccg gaacgcgtcc cgatgcgggc
     2881 ggctgccggc gggagcgtgc ccggaggtcg ctgaacgccg tgaagatggt cttgacggcc
     2941 tggaagatcg ccatgccgtc gcgcgccggc ccgaggatgg tctgctggac gacggccatg
     3001 gtctcttcga cccgcctcga ggcgtcgttc agcaggcgtt ccgcccgctc gacctgtgcc
     3061 gtcgcgatcg ccgccgcgcg cgccgcgtcg gccgacatcg cctggacgtt ggcgacgatc
     3121 ggccggacgt cctgctcgag cttgctcacg gtctctccga gccgccgagc cgcctgggcg
     3181 gcaaccaccg ccgcccacac ctggaccact gccatggcga ggaccgccac ggcaatcaga
     3241 cccaggaaga actcggtcag gttcacgcgt cctgcccggt ctcgcgcgtc gcctggaact
     3301 gctcgcgcgc gcggtcgaac gccgtggtga ggttctcgcg ctgcttgctg atcatctcgc
     3361 ggctctgtcg cgccgcttca gccgcgcggt cgcgcccctc gcgcgcacgc tgccccagaa
     3421 actcacgagt ctcttctccc gttgccgggg cgaagagcaa ggccacggcc gcgcccgcaa
     3481 tcgcgccggc gacaaacgcg aggaacatgc ccccgccgct tccctgctgt gacatgactc
     3541 aactcctgta gattcgccgt ctcagaacag gctcactttg acgttgaggt acttcttcgg
     3601 gtctcgacgg atgtcgatga ccaatccccg aagttccgcc atcgtctccg taatattgtc
     3661 atacaacttc ttgtcgcgca gcagctgccc ggccgtgcct tcgccctgct gaagtccacc
     3721 caccagcttg tccagccggt ccgacagcga attgaaacgg tcgtacagct gcgcatcggt
     3781 gacgagcttc cctgccgtgc cttcaccgcg attgatcctg ccggtgatgg cgtcgaggtt
     3841 ggctgtcgtc gaggtcagcg accggctgag cgcgtcgttg ctcaggagct gtccgagcga
     3901 gccctcccca gcgcgaatgc gcgccgtcac ggcctgcaga ttgtcgagcg aggcctcgag
     3961 cgccttcgcg gccgcgggat cgctggccag acggcccagt gttcctcgtc ccttgttgat
     4021 gccgctcgcg acgtcctcgg cggagctcac gagcgcggtg agctcctggt agagcctgtc
     4081 gtcggtcatc aactgcccca tcgtgccgcg accggcccgg atgctgctga cgagcgccgt
     4141 ggcctcctcc atggcgacgc ccgcttgcgt ggcgacatcg ccaatcgttc ccatggcgcg
     4201 cccggacggc acgtatcccc attcgggaat gggctgaccg gtgctcgagg ccgtgatgtc
     4261 gagtgccgct tcgcccagga gcgacaccga ccccaacgtg gcgatcgagg ccgtcgtgat
     4321 gcgtgactgg ttgccccgtc ccacttccat caggacttcc acgcggtcgc cggtgaaggc
     4381 gatcccggtc accgaaccga tctcgacgcc ggcaagccgg accggcgcgc cctccttgag
     4441 gcccgggatg ctgtcaaaca cggtcttcag cccgacgcgc tgccagaaga agccgccctc
     4501 gccaccgatc atgaagatca tgatgaccga caggatgacg gccaggacgg acacgacgcc
     4561 gatcttgagc tcggcccagg cgagtgaacg tgtgcggggc ataggtcact cctcacgaaa
     4621 gaaacgtctt gaggtaggaa tcgtgcgtgg cgcgaagctc gctggcattg ccttcgaacg
     4681 ccactcgtcc gttcctcaac atgatgaatt ccgcctcatc ggctttctgc tcgtccgccg
     4741 cgacgatgcg aacgcggctg ccatctctga tcgcttcgtg cgtcgcgatg tagaaagcgt
     4801 cgcgcagctg gtgcgtgacg atgatcgagg tcaccctctc gaggtcgcgc agcttgatga
     4861 tctcctcgtc caccgtgatg gcagtgatgg gatcgagccc ggtggtggct tcatcgtaaa
     4921 ggatgatgcc cggcttggcc gccatcgcgc gcgcgatcgc gacgcgtcgc cgctgaccac
     4981 cggacaactc ggacggcatg ttgtcgatgt agtcggcgag tccgatgaag ccgagcacct
     5041 gctcgacgcg ccgcctcaca tcgccgggtg gcgtgcccga ttcctcgtag agcttgtagc
     5101 cgacgttttc cgcgacgctc agcgaatcga agagcgcgcc ctcctggaac accatcccga
     5161 tatcggcgcg tacccgcatc aagtcccgtt cgctcatcgt atccgtacgc tcgccacgga
     5221 cccggatgat gccgccgtcc ggcttccaga agccgagaat cagcttcaga atcgtcgact
     5281 ttcccgatcc gctggcgccc agaatgatct tcgtatggcc gggcagcagc gtgaagctca
     5341 cgtcctgaag cacgaccgac tcgtcgaatg acaacgacac ccgtgcgagc tcgatcgcag
     5401 gactctcgac gagctgaact cccgtcggtt gccggtcttc ggtcggttgt cggtccattc
     5461 gtcggtcttc ggtttccggt ctagtacaac aacgtgatca gtagtcgcgt aaggaagaag
     5521 tccacggcga tcaccgctac tgacgacgcc acgaccgcgt tggtcgtgga gcggccgacg
     5581 ccctgagttc cacctttcgt acgcagaccg acgtggcatc caatcgtcgt gatgacgtag
     5641 cccagacaga agggcttgat gagccccatc cacgcgtccg ggagggccaa cacatcgacg
     5701 atggaggtcc aatagagact ggaggccacg cggagctgaa atacggcaat gaaccatccg
     5761 ccgaggatcc cgagggcatc ggcgaggacg gtgagcaccg gcgccatgaa caggccggcc
     5821 agcacccggg gcacgaccag cttccgcacg gggtcggtgc cgagcgccct cagcgcgttg
     5881 atctggtcgg tcacgaccat cgacccgagt tcggcggcga tgcccgagcc gatgcggccg
     5941 gccagcatca gtccggtcaa gaccgggccc aattcccgga tcatcgatgc gctgaccagc
     6001 tggcccacga acggccgcgc gccgaagttg tcgagcgtga ttccggtctg cagcgccagc
     6061 accgcgccgg tgaagaagcc ggtcagcacg acgaccgtca gcgagccgat cccgatctga
     6121 tcgaactgct ccacgacgtc gcgcgcgtag aagggacgcg aaacggcggc ctgcagcgcg
     6181 gccgcctgca ggcgtacgta ctcttgaacc tcgaggaccc cgcccttcag ccatgtcgtc
     6241 aggatgtcca gcactaccgg cccgccgtga tgccggagaa gccgaggtcg cgcgtgcgca
     6301 gtgccttcag ccggtcgcgc agggccgccg cgcgttcgaa atcgaggttg gctgccgctg
     6361 cccgcatgtc cttttcgaga cgcgtgatct cggcgtcgag ctcggcctgg gtccggaacg
     6421 tctcctgcgg agtctcggcg ggggtcatgt agtcacgctc gtacacactc gccatgacgt
     6481 cgtcgatctg acgaacgacc gactccggcg tgatcccgtt cgcctcgttg taggcgcctt
     6541 gcagcgcgcg acgcctctcg gtctcggcga tggccaaccg catggatgcc gtcatggtgt
     6601 cagcgtacat gatgacgcgg ccgttgacgt ttctggcagc gcggcccgag gtctgaatga
     6661 gcgacccagc ggagcggagg aagccctctt tgtccgcatc gaggatggcg accagggaca
     6721 cttccggcag gtcgagaccc tcgcgcagca ggttgatgcc gacgagcacg tcgaacgcac
     6781 cgcgccgcag atcgcgcagg atctcgaccc gctccagcgt gtcgatgtcc gagtgcaggt
     6841 agcggacctt gacgccgagc tcctggtagt aggtcgtcag gtcctcggcc atgcgcttgg
     6901 tgagcgtggt gacgagcacc cgctccttcc gtgccgcacg gtcgcggatc tctccgagca
     6961 gatcgtccac ctgcccgcga accggcctga cctcgatcgg cgggtccatc aagccggtcg
     7021 gccgtatgac ctgctccacg accactccga gcgacctgga cagctcgtag ggccccggcg
     7081 tcgcggatac gaagacgacc tgcttcaccc gctgctccca ctcctcgaag ttgagcggcc
     7141 ggttgtcgag cgctgacggc aagcgaaagc cgtaggcgac cagcacttcc ttccgcgagc
     7201 gatcgccctg gtacatcccg cgaatctgcg gcacggtctg gtggctctcg tcgacgatca
     7261 ccagcgcgtc gtccggaaga tagtcgagca gcgtcggcgg cggagcgccc ggcggccggc
     7321 cggtcaaatg gcgggcgtaa ttctcgatgc cgtggcaata gccgatctcg cggatcatct
     7381 ccaggtcgaa catcgtccgc tggtgcagcc gctgcgcctc gaggagcttg ccctcggact
     7441 cgagcagcga gcgccgctcg gccagctccg ccttgatcgt ctcgacggcc tgcttcgtac
     7501 gatcgcgcga ggtgacgaag tgcgacttgg ggtacaccgc gaccttgtcg agccgtcgaa
     7561 tgaccttccc ggtcagcggg tcgaaccagg cgagctcgtc cacctcgtcg ccgaacaacc
     7621 cgattctgag ggcgtgctct tcgtaggaag gcaccacctc gacgatgtcg ccgcgcacgc
     7681 ggaacgtgcc gcgaccgaac tcggtgtcgt tgcgctcgta ctggatctcg accagcttgc
     7741 gcaggatctg gtcgcgctcg atgcgctgtc cgagctcgag aggcaggagc aggccgtagt
     7801 aggcctcggg cgagcccagg ccgtagatgc acgacacgct tgccacgatg atgacgtcgc
     7861 ggcgctcgaa cagcgatcgg gtggccgaca gccgcatccg atcgatctcg tcgttgatcg
     7921 tcgcttcctt ttcgatgtac gagtcggtcg caggaacgta cgcctccggc tggtagtagt
     7981 cgtaatagct cacgaagtac tcgacggcgt tctcggggaa gaaccgcctg aactcctgga
     8041 agagctgtgc cgccagcgtc ttgttgtgcg ccatcaccag cgtcggccgg tgcacctgcg
     8101 cgagtacctg cgccatcgtg aaggtcttgc ccgagcccgt tacgcccagc aacacctggt
     8161 gcgtgtcgcc ccggttcagc ccctcgacga gctcgggcac cgcatggacc tggtcgcctc
     8221 gaacctcgaa gtcgctgacg agcctgaagc ggtcgtgaac ggaaggcatg tgcgtcgtcg
     8281 tacggagcag gcagccggca gccgctagtt cggccggtac ggcgcgaagt agccccgtct
     8341 cgcctgaatc cgggtaccct tcgcaaccga ccgcggcacg cggatttcga ccttgcgcca
     8401 gcgtccgtcc gccgccgtgt tggtagagac gtaccccagt gtgtactgcg cttgcacctg
     8461 cgcgacgatg gtgtcgtagg cttcgtccac ctgcttgacc gaggagggaa agaa
//
LOCUS       DBNGSH010000827         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2473369, whole genome shotgun sequence.
ACCESSION   DBNGSH010000827 DBNGSH010000000
VERSION     DBNGSH010000827.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2473369"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..831)
                     /locus_tag="AC16CY_19030"
     CDS             complement(1..831)
                     /locus_tag="AC16CY_19030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3355952.1"
                     /translation="MRSVSSRRNPLVARFRELTDEPDPSGSRLLLDGLHLVSDARASG
                     IAFEVVAVAGSRLSSDTKEGSLGRTLSAAGVDVVEAPDAVFASLSPVNTPSGIVAIAQ
                     RPSVTAEEICAAADAFVVVAVDVQDPGNVGALVRVAEAGGATGMLVCGGSASPFSWKA
                     VRGSMGSVLRLPVASGLTAAEALARLRRSGVRAVASVTRGGLDPHAFDWRGKAAILLG
                     GEGAGLTQEIVDACDARVSIPMAPEVDSLNVATAGAILVYAARRLSTRKASGVRRKAQ
                     "
     gene            907..1371
                     /locus_tag="AC16CY_19035"
     CDS             907..1371
                     /locus_tag="AC16CY_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR3355953.1"
                     /translation="MKEKILKRFNDEIAVLERELKIDLPKEIQRAREHGDLRENAEFK
                     AAKERQEIVNARIGMLKKRAGEIAMMNLDRIPHDRAGFGSTVELRSTAGDLVVYTLVM
                     PEEADAEKGLISTSSPIGRAILNKEEGDDISVTTPGGSKRFELVKLTTIHDE"
     gene            1403..>2685
                     /locus_tag="AC16CY_19040"
     CDS             1403..>2685
                     /locus_tag="AC16CY_19040"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3355954.1"
                     /translation="MTPYSPSRRTALVLTGTGAHGAYHAGVLRALQEAGVKIDLVAGH
                     GVGAGSAALAAIDGAARLWEPTGIWRSRVTGLYRWRWPLRAAAWIGVILMAVLLTPIF
                     VLVLGLVVYLLGFLFEMLRLDAGKTLADAYSGWLQVAFAGENLPTMVPRLVMLALASI
                     LAVLAWGGVLAGRAIGARRGERRWWWRVIAAPLDAQAARERFTAAVWDLIRGAAPVAR
                     PARTTLGRRYSEVLVENLGQPGFRELLLVATDLDARRDVVAALLAEPHRQSFLAHRPG
                     RDRRSEVLDLAGTARDHVIDLIGAAVTPPVLCDPQLVTFAADSFWRGETHRLCDRPGA
                     VERLLEEAAEAGATQAIVVTAVAPTAAPHRLRAPRLDVRGRFGEFHCAAEAAALRDAL
                     EALTPRFDSVYVIQPGHNPIGPFDVSGAYDEASDRA"
BASE COUNT          418 a          907 c          930 g          430 t
ORIGIN      
        1 tcattgggcc tttcgcctga cgcctgacgc ctttcgggtc gatagccgtc gtgcagcata
       61 gacgaggatg gctccggcag tcgcgacatt cagcgagtcc acttcgggtg ccatcggaat
      121 cgagacgcgg gcgtcgcacg cgtcgacgat ctcttgtgta aggccggcac cctctccgcc
      181 aaggaggatg gccgcctttc ccctccagtc gaacgcatgt ggatcgagcc cgcccctggt
      241 gacactggcg acagcgcgga cgcccgaccg ccgcaggcgc gccagcgcct cggccgcagt
      301 caggccggag gcaaccggca gccggagcac gcttcccatg cttccgcgaa cagctttcca
      361 ggagaagggg ctggcagaac cgccacacac cagcatgccc gttgcgccgc cggcttccgc
      421 gacgcgcacg agtgcgccga cgttgccggg gtcctggacg tccacggcga cgaccacgaa
      481 cgcgtctgct gcagcgcaaa tctcctcggc cgtcacgctg ggccgctgcg cgatggcgac
      541 gatgccggat ggcgtgttga cggggctgag cgaggcaaag acggcgtcgg gcgcctcgac
      601 gacgtccact cccgcggcgg acagcgttcg gccaagactc ccttctttcg tgtccgatga
      661 cagccgcgag ccggcgacgg cgacaacctc gaatgcgatc ccggacgcgc gtgcgtcgct
      721 caccaggtgg aggccgtcca ggagcaggcg tgagccggac gggtccggct cgtcggtcag
      781 ctcgcggaag cgggcgacga gcgggttacg ccggctgctg acggaacgca cttaagaacc
      841 tactgcaccc tcgcaccttc acaccctcgc acctttgacc cattgtgcta cattctgatt
      901 tcgacgatga aggagaagat cctcaagcgc ttcaacgacg agatcgcggt actcgaacgc
      961 gagctgaaga tcgatcttcc gaaggagatc cagcgggcgc gcgaacacgg cgatctgcgc
     1021 gagaacgcgg agttcaaggc cgcgaaagag cgtcaggaga tcgtcaacgc ccgcatcggc
     1081 atgctgaaga agcgggccgg cgaaatcgcg atgatgaacc tcgatcgcat tccgcacgat
     1141 cgcgccggtt tcggttcgac cgtcgagctg cgttccacgg ccggcgacct cgtcgtctac
     1201 accctggtga tgccggaaga agcggacgcc gaaaaaggcc tcatctcgac ctcgtcccct
     1261 atcggacgcg ccatcctcaa caaagaggag ggcgacgaca tcagcgtgac gactccgggc
     1321 gggtcgaagc gtttcgagct cgtgaagctg acgacgatcc acgacgagtg acccgtgaac
     1381 ggactgtact gagttcatcg atgtgacccc gtactctccg tcgcgccgga ccgccctcgt
     1441 gctgacaggg acgggcgcgc atggcgccta tcatgccggc gtgttgagag cgctgcagga
     1501 ggccggcgtc aagatcgacc ttgtcgccgg gcacggcgtc ggcgccggca gcgccgcgct
     1561 ggcggcgatt gacggtgcgg cgcgcttgtg ggaaccgacg ggtatctggc gatcgcgcgt
     1621 gacggggttg taccggtggc gatggccgct gcgcgccgcc gcatggatcg gcgtgatcct
     1681 catggcggtc ctgctcacgc ccatcttcgt gctcgtcctg ggcctggtgg tctacctcct
     1741 tggattcctc ttcgagatgc ttcggctgga cgcgggaaag acgctggccg acgcctactc
     1801 gggctggctg caggtcgcct tcgccggaga gaacctgccg acgatggtcc ctcgcctcgt
     1861 catgctcgca ctggcgtcga tactcgccgt ccttgcgtgg ggcggagtgc tggcgggccg
     1921 ggcgatcggg gcgcggcgcg gggagcggcg gtggtggtgg cgcgtcatcg cggcgccgct
     1981 cgacgcccag gccgcgcgcg agcgattcac cgccgcggta tgggacctca ttcgtggggc
     2041 ggcgccggtc gcccgccccg cgcgcacgac cctcggccgg cgatactcgg aagtgctggt
     2101 cgagaacctc gggcagcccg gctttcgcga actgctcctc gtcgcgaccg acctcgacgc
     2161 gcggcgggac gtggtggccg cgctgctggc cgaaccgcac cgccagtcgt tcctggccca
     2221 ccgtccgggc cgcgatcgac gatcggaggt gctggatctg gccggaacgg cacgcgatca
     2281 cgtgatcgat ctgattggcg ccgcggtgac gccgcctgtg ctgtgcgatc cgcagctggt
     2341 gacgtttgcc gccgacagtt tctggcgcgg cgaaactcac cgactgtgcg atcgtcccgg
     2401 cgcggtggag cgcctgctcg aggaggcggc agaggcgggc gcaacccagg cgattgtcgt
     2461 gactgcggtg gcgccgactg ccgcgccgca tcggttgcgt gcgccgcgtc tcgacgtgcg
     2521 agggcggttc ggcgaattcc actgcgcggc ggaagcggcg gccctccggg atgccctcga
     2581 ggcgctgacg ccgcggttcg actcggtcta cgtcatccag cccggccaca acccgatcgg
     2641 ccccttcgac gtctcgggtg cgtacgacga ggcctccgac cgcgc
//
LOCUS       DBNGSH010000828         3472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1624631, whole genome shotgun sequence.
ACCESSION   DBNGSH010000828 DBNGSH010000000
VERSION     DBNGSH010000828.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1624631"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="ligD"
                     /locus_tag="AC16CY_19045"
     CDS             <1..1009
                     /gene="ligD"
                     /locus_tag="AC16CY_19045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HIR3355955.1"
                     /translation="VGRGQGAGGSRAGRRGKGAGGSVEYVPQLTTLVKTPPSGDEWLH
                     EIKLDGYRIGCLIDGGRVRLVSRNGQDWTGRFPEIADAARKLPLDSAVIDGELVVLEP
                     DGRTNFEAMQRAASGRPPRAGLVYFAFDLLRLDGERIDRLPLETRKARLLKLVGDGKT
                     GRIRYTEHVQGRGSEFFEQARRLGLEGVISKRRDLPYQPGTRDGWRKTKCLKQERFVI
                     GGFTDPEGSRAGFGALLVGRRDGSRLVFSGRVGTGFSHALTLDLRRRLDALARKTPPF
                     DPPPPPQFAKNAHWVEPSLACEVTFIERTDAGVLRHPSFQRLVTQDSTLKTHNWKSSH
                     R"
     gene            complement(1041..>3472)
                     /locus_tag="AC16CY_19050"
     CDS             complement(1041..>3472)
                     /locus_tag="AC16CY_19050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355956.1"
                     /translation="FALAAIACLALGIGANALIFSLVNAAFLRPLPYPDADRIAMVRF
                     TPPNQPDEKLGTNSGGYFFIREHNRVFERMGVLRLTGITAAVGDSDDAQREWLQVGWA
                     SPGLADVFGVQPVIGRWFRPDDLEVGVVITHGVWQRLFGGRPDVLGQTMRFDAMRGVV
                     IGVTPPGYRTLTPDVDLWIQQTDENLARALRSPNRPFNMFARLRPNATLEDAQADLRT
                     LERPLGKDLPMHQGWGLTIDSLREVYVGYLRQPVLVLQGAVFLLLLIACANVGGLLLS
                     QAVARQKELGVRAALGSSRNRILRQLLTENVLLSCAAGVLGIVLAWIGLRAFVNTGLS
                     AYRDLQNVTLDWTVIGFAIVVSLATALVFGILPALQLSRLDVVEVVRDAGRGATAGRT
                     RSRLRGAFVVAQVALALVLLVATGLLTRSLLRLNAVDTGIDPERLLALEIPMPRALYR
                     NTLANTPAGGLLVEFDSRFSDLTERLRERFASVPGVQSVAATTPPPLGGTPRRVLFRK
                     DSWLALADDREPWSAEWYPVSAGYFETVQIRVLQGRTFTRQDAQSTPPVAIINASLAA
                     RYWPNDNPIGRLLQTDVLNDPPREIVGVVGDVRQDRYQSAPVPQVYVPRTQLPYRMDM
                     TMSLEVLVTTFVVRAAGDTVSLVPALRAAVRDVDATLSVSSARTVEDYAADQLQELSQ
                     YAAVLGVFGAMSVTLAVIGILGVMAQAVGQRANEIAVRMALGAQSLNVLGLVLRQGLA
                     MIAAGLGVGVVASLMVMPVIRSFLWGVTITDPLTLALVVLALAAVALVACYLPARRAL
                     KVAPIAALRGD"
BASE COUNT          624 a         1191 c         1173 g          484 t
ORIGIN      
        1 agtgggcagg gggcaggggg cagggggcag tcgggcaggg cgcaggggga agggggcagg
       61 gggcagcgtc gagtacgttc cccagctcac cacactggtc aagacgccgc cctcgggcga
      121 cgagtggctc cacgaaatca agctggatgg gtaccgtatc ggctgcctga tcgacggcgg
      181 ccgcgtcagg ctggtcagcc gcaacggcca ggactggacc ggccgcttcc ccgagatcgc
      241 cgacgccgcg cgcaagctcc ccctcgacag cgccgtgatc gacggcgagc tcgtcgtgct
      301 ggagcccgac gggcggacga acttcgaggc aatgcagcgg gccgcgtctg gccgcccgcc
      361 acgcgcgggc ctcgtctact tcgcgttcga cctgctccgc ctcgatggcg agcgcatcga
      421 tcgcctgccg ctcgagacgc ggaaggcaag gctcttgaag ctggttggcg acgggaagac
      481 cggacggatc cggtacaccg agcacgtgca gggacggggc agcgagttct tcgagcaggc
      541 gcgccggctc gggctcgaag gcgtcatctc caagcgccgg gatctgccct accagccggg
      601 aacgcgtgac ggctggcgga agacgaagtg cctcaagcag gagcgcttcg tcatcggcgg
      661 cttcaccgat cccgaaggct cgcgcgcggg ctttggcgcg ctgctcgtcg ggcgccgcga
      721 cggctcgcgg ctcgtcttct ccggccgcgt cggcacgggc ttctcgcacg cgctcacgct
      781 cgacctccgg cgccggctgg acgcgctcgc acggaagacg cctccattcg acccgcctcc
      841 gccgccgcag ttcgcgaaga acgcacactg ggtcgagccg tcgctcgcct gcgaggtcac
      901 gttcatcgaa aggaccgatg cgggcgtgct caggcatccg tcgtttcaga gacttgtaac
      961 tcaagactca acactcaaaa ctcacaactg gaagtcatct catagatagc cggcattgga
     1021 ctggacacca gccggacgcg tcagtcgccg cgcaacgccg caatcggcgc caccttcagc
     1081 gcgcggcgag ccgggaggta gcaggccacc agtgccacgg ccgccaacgc gaggacgacc
     1141 agtgcgagcg tcagcggatc cgtgatcgtg acgccccaca gaaagctgcg tatgacgggc
     1201 atcaccatga gggacgcgac cacaccgacg ccgaggcctg cggcaatcat cgccagccct
     1261 tgccggagca cgagtccgag cacgttcaac gactgcgcac cgagcgccat acggacggcg
     1321 atctcgttcg cgcgctggcc gaccgcttgt gccatgacgc cgaggatgcc gatcaccgca
     1381 agcgtgacgg acatcgcgcc gaacactccc aacacggcgg cgtactggct caattcctgg
     1441 agctggtccg cggcgtagtc ctccacggtt ctcgcgctcg acacgctgag cgtggcgtcg
     1501 acgtcgcgaa ccgcagcgcg cagcgcaggt accagcgaga cggtgtcgcc agcggcacgc
     1561 acgacgaacg tcgtcacgag cacctcgagg ctcatcgtca tgtccatccg gtacggcagc
     1621 tgcgtccgcg ggacgtacac ctgtggcacg ggcgctgatt gataacggtc ctgtcggacg
     1681 tcgccgacga caccgacgat ctcacgcggc ggatcgttca gcacgtctgt ctgcaggagc
     1741 cgtccgatcg gattgtcgtt cggccagtac cgtgcggcca ggctcgcgtt gatgatggcg
     1801 accgggggcg tggattgtgc gtcctggcgc gtaaacgtcc gtccctggag cacgcgaatc
     1861 tgcacggtct cgaagtaacc ggcgctgacc ggataccact ccgccgacca gggctcgcga
     1921 tcgtcggcca gggccagcca cgaatccttc cggaacagca cgcgccgcgg cgttcctccc
     1981 agcggcggcg gcgtcgtcgc agcgacggac tgaactccgg gcaccgaggc aaaccgctcc
     2041 cgcagccgct cggtgaggtc gctgaagcgc gaatcgaatt cgacgagcag cccgcccgca
     2101 ggcgtgttcg ccaacgtgtt gcggtagagc gcgcgcggca tcggaatctc gagcgcgagc
     2161 agccgttcgg ggtcgatccc ggtgtcaacc gcattcaaac gcagcaggct ccgggtgaga
     2221 agcccggtgg cgacgagcag cacgagcgcc agagccacct gcgccaccac gaatgcccca
     2281 cgaagccgac tgcgcgtccg gcccgcggtc gcgccccgtc cagcgtcgcg aacgacctcc
     2341 accacgtcga gacgcgaaag ctgcagcgcc ggcaggatgc cgaagacgag cgccgtcgcc
     2401 agcgagacga cgatggcgaa gccgatcacc gtccagtcga gcgtcacgtt ctggagatcg
     2461 cgataggcgg acagtccggt gttgacgaac gctcgcagcc cgatccacgc gagcacgatg
     2521 cccagcacgc cggcggcgca tgacagcagc acgttctcgg tcagcaactg gcgcaggatg
     2581 cggttgcgcg aggacccgag cgcagcccgt acgccgagct ccttctggcg agcgactgcc
     2641 tgcgacagca gcaacccgcc cacgttggcg cacgcaatca gcagcaggag aaagaccgcg
     2701 ccctgcagca cgagcaccgg ttgccgcaga tagccgacat agacctcgcg gagcgaatca
     2761 atggtcaatc cccacccttg atgcatcggc aggtccttgc ccaggggccg ctccagtgtc
     2821 cgcaggtcgg cctgcgcgtc ctccaatgtg gcgttcggac gcagtcgggc gaacatattg
     2881 aacggccggt tggggctgcg gagcgcccgg gcgaggttct cgtccgtctg ctggatccag
     2941 aggtccacat cgggtgtcag cgtccggtag ccgggaggcg tgacgccgat gacaacgcct
     3001 cgcatcgcgt cgaagcgcat cgtctgtcca aggacgtcgg ggcggccgcc gaagagccgc
     3061 tgccagactc cgtgagtgat gacaacgccc acctccaggt catcgggtcg gaaccagcgg
     3121 ccgatgaccg gctgcacgcc aaagacgtcg gcgagtcccg gcgacgccca cccgacctgc
     3181 agccactcgc gttgcgcatc atccgaatcg ccgacggcgg cggtgatccc cgtgaggcgg
     3241 aggacgccca tccgctcgaa cacgcggttg tgctcgcgga tgaagaagta gccgcccgaa
     3301 ttggtcccca acttctcgtc tggctggttg ggcggcgtga accgcaccat cgcgatgcga
     3361 tcggcgtctg gatacgggag cgggcgcagg aacgccgcgt tgaccaggct gaagatcagg
     3421 gcattcgcgc cgatcccgag tgccaggcag gcgatggccg ccagggcgaa cg
//
LOCUS       DBNGSH010000829        10853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1794892, whole genome shotgun sequence.
ACCESSION   DBNGSH010000829 DBNGSH010000000
VERSION     DBNGSH010000829.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1794892"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="AC16CY_19055"
     CDS             complement(<1..880)
                     /locus_tag="AC16CY_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355957.1"
                     /translation="MRHTLALCGAAALFFSPVLAGTLLGQQDGQPPQRPSVQVGVPEG
                     REGAAGRQGGRGRAAGPPAQPAPRGPDGRVLLTSATPTQKGVWQPGGGGGAAPNAERQ
                     IPFQPWAREVYADRGRHQLEPHTRCKPSGGIRQFITPYGVEIVELRELQRIYIFDIGG
                     PHTYRTIFMDGRTHPENLTPTYYGHSIGWWEGDTLLVETTGFNEGFWIDRRGLPTTEK
                     LRTLEKFTRADSLTINYELTIDDPGAYTAPWTSGLSLRWEDGTELFEYVCQQANYAHN
                     LMLGQYESVDRTTLAVP"
     gene            complement(917..1333)
                     /locus_tag="AC16CY_19060"
     CDS             complement(917..1333)
                     /locus_tag="AC16CY_19060"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355958.1"
                     /translation="MRAYILGGVVAAGLMFAAGSAVAHHSFAAEFDANKPITLTGRVT
                     KVEWTNPHVWFYINVTDEKTGEVSNWGAEMGPPHGLQRRGWRRETLKIGEEVKVSGSL
                     AKNGSKKVNARSVTLLATGGRPGETLDAVSSQGSNP"
     gene            complement(1368..2489)
                     /locus_tag="AC16CY_19065"
     CDS             complement(1368..2489)
                     /locus_tag="AC16CY_19065"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355959.1"
                     /translation="MRFVVALAIAVVLALTLGAASGAAHHPISAKFDDTKPLTLTGVV
                     TLVDWRNPHVHIYMNVRRGNDADNWAVELQSPIDLQTGGWNSESVQPGNSITVTGIAA
                     RNGSRQVWANRIVMTGTGRQVLNVAPPSPPALLQNRPTPKWADGQPRLGPIPGGVQGY
                     WAFPSSTVLAENGSNVQMDQYGLLRNVADARRVAPMQPWALALYELRQRRFLQDDPTY
                     LNCKPPGGPRQFQLSNGVQLVEDRERQRIFVLIGGGNNNFRIIYLDGRGHAGQVGGDD
                     DNPLYYGRAVGKWEGNTLVVDTRGFNEDFWFTNGGLPHTEQLRLVERFTRENFDTLKY
                     EVTIEDAGAYTRPWSSGWTLRWVTSSEDMPRHLCQENRP"
     gene            complement(2499..2900)
                     /locus_tag="AC16CY_19070"
     CDS             complement(2499..2900)
                     /locus_tag="AC16CY_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355960.1"
                     /translation="MRALTIVLTLLVACLSGGVSVTAQEGHPLKGSWLGTWGPAKSHS
                     NDIVLVLNWDGKTISGTINPGVNDAPIKNATLNPDGWVVHLEADIKEKSGAVLNYVID
                     GKIENLSFHNRAIVGTWKNQRESGQLKISRQ"
     gene            3046..3549
                     /locus_tag="AC16CY_19075"
     CDS             3046..3549
                     /locus_tag="AC16CY_19075"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR3355961.1"
                     /translation="MARHSACLRLTLLFVVAWVCVSPPARVAAEAPPAAGGEVREMVR
                     LTNAERSRAGRAPLAASPRLMKAAQLHAEQMVARGQMAHDLPGARYPQTEDRLAAAQY
                     RWLSYAENVAYGQPDPVSALDSWMDSPGHRRNILNASMTELGTGFARDRSGRPYYVQV
                     FGRPRSR"
     gene            3673..3954
                     /locus_tag="AC16CY_19080"
     CDS             3673..3954
                     /locus_tag="AC16CY_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355962.1"
                     /translation="MVITRVGPLSVAKVAGLLYGIIGLLFGVFISLFAMAGFAAGAND
                     SPFRAVFGVGAVILLPIFYGCLGFVMTLIMSWLFNIVVGITGGVEVDAR"
     gene            complement(3923..4876)
                     /locus_tag="AC16CY_19085"
     CDS             complement(3923..4876)
                     /locus_tag="AC16CY_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3355963.1"
                     /translation="MRRTLLGVLLATTLAPWLAAQSTRPVVLSRLLWFDRDGQRLGAL
                     GPVADHGNLELSPDGRRVAVAVTDRSSVSRDIWIYEVDSDSRTRFTTDAADENWSIWS
                     PDGARMLFNSFAPGRLGLFERPAKGSGVRNELLAGADGIWPLSWSPDGRSVLYVTNSE
                     RTGNDISVLSLDGSSRTSPFLRTEEAENWAAFSPDGRWVAFSVTVSGRAEVFVSAFPP
                     TERRWQISAEGGFQARWRRDGEICYVAPNRMLMAASVSASGAEFSVTRVEELFQLSFP
                     NGAYHAFDVTRDGTRFLVNTLVVNPAERGLSVSARPPPRLR"
     gene            5029..6510
                     /locus_tag="AC16CY_19090"
     CDS             5029..6510
                     /locus_tag="AC16CY_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355964.1"
                     /translation="MIVHINHISQSKKPKSSQRSPQSEMAGLVTGSLMSLVEDGVLDA
                     RLAPSLRIHLDWIQYKTNFRDPVMVRRTTNAQGQQMPLAEIAIDLRQADAGRLQDDLA
                     RAVKSLGAGTESNTESHLFLDDFRPYRDCLMWSFNRLFWRHLGDWEAASGKSFEAALP
                     GGSSDANHPQAVSDSVADFWTLLRDLEARGKLPPEIFGLEIGVGSGARAAAWLDRFKA
                     LDEQCGTSFYSRMHFILGDYSPTSLERALAAVAHHGSHASGVPLDALNPFKSLGPYRF
                     KIMYVHLTNVYDNLPFDDLVRRDAKLYLVEVRPYLNGPMVDRLTADFGVTRADLPGLV
                     TTLLDAGPHAVFPGEKGVLFWRRLWDTFKLEERLRVLDDREEDHVPPGLSRTHLEDLL
                     AEAPDDVRFHISRGAGESFANTLPLLHPRGFLQVQDIFVSEMDDYRKGFKGPGKLDGS
                     LVTWVNGALLRAVGARAGYDVHFAPFPYRPGTKTSILYTTQRD"
     gene            6661..7662
                     /locus_tag="AC16CY_19095"
     CDS             6661..7662
                     /locus_tag="AC16CY_19095"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR3355965.1"
                     /translation="MTPPLPILPTTVIGSYSMPEWLERAKNEYLSRRLSRRDLDEMHD
                     AVRKAAIKDQESAGIDIVSDGEAQRDNMIDYFTERMPGVQVDLGSKRFYYDFYESVVR
                     SKLATGTLGLAEEARFLARFSELGSKISISGPHTLVKRIQNKHYATEEAFALDLGHVL
                     NLELKELVKAGATRLQIDEPYYSGFPEDLPWALKAINAMVDGVNAHVTLHICYGNRYG
                     KPSFEGSYEFLFPTIMEARVQAISMEFARRGEDDIKLFKTVPFQVGIGVIDVKTNEVE
                     TAAFVADRIRRALEVIPAERLIITPDCGLVRLPREVAFAKLSAMVEGTRIVRKELGK"
     gene            7659..9257
                     /locus_tag="AC16CY_19100"
     CDS             7659..9257
                     /locus_tag="AC16CY_19100"
                     /inference="COORDINATES: protein motif:HMM:NF014296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3355966.1"
                     /translation="MRPLAERVKSGQFVYMTELVASGLKREAQVLEIASNLARIPEIA
                     AGSITSYAGGQLGQDSIRVGTAVRARGLVPNVHLTCVGQDRTTIDRTLAALRALEMHN
                     IFSISGDWPKAAMRTAVFDIDSVQLAAHIADLRARTGTPFFISCAVSPFKYQREDLLY
                     QYLKLEKKIAAGADMAITQVGWDATKFVELKKYLDERGIRTPVLGNVYVLSRRAAERM
                     NTGNPPGCWASDELVAVLRKESEAPDGGLQARLERAARSVAVLKGIGYAGAYIGGTHD
                     AKHVTHIIRRAEELAPKWQQLFEELRFGKKDGFYLYKRDPHPKPRRTFLPTVMDAAGK
                     TLPFVPWSKTVRDSLMRRALQGTFRWLDRHPRLSHWFERFEYYSKKEVFGCQNCGNCV
                     LGHMEYVCPQTCPKQMRNGPCGGTFHGACEVIDQECIWVKVMERAEASNTIDQLKVYI
                     PPPDRTLTGTASWLNFYLGRDSRPDRPKERWESGGPGRTLAIEEDEKLQTAGPPAADK
                     ALAARTAKTPGKVEAPAKAEKATA"
     gene            9274..>10853
                     /locus_tag="AC16CY_19105"
     CDS             9274..>10853
                     /locus_tag="AC16CY_19105"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR3355967.1"
                     /translation="MKSALEIAHEAQLRPIADVAASAGILPDELEQLGRYRGKVRLSI
                     LDRLATRPNAKLVIVTAITPTKAGEGKTTTSVALTQGLGKIGKKVMLCLREPSMGPVF
                     GVKGGGTGGGYSQIGPMEDINLHFNGDFHAVTAAHNLLAAAVDASIFNGNPLRIDPST
                     VIWPRTLDMNDRELRYTVVGLGGKTHGVPRENQFIITAASEVMAVFALAGSLADLRRR
                     LGRIVVASNYEGEPVTAEMLKVAGAMTVVMKDAIKPNLVQTLEGQPALVHAGPFGNIA
                     HAANSILADRIAIKSADYVVTEAGFASDLGFQKFCDIVCRVGGFAPSAAVLVTTVRAT
                     KSHGGKSFNVLANEDLDSIAKGADNLAAHVQIVRQYGLPCVVAINSFPTDTARELALV
                     RELALKAGAETVVTHSGFVEGGAGATDLAKAIVSACDKPNTFSHLTPDGTPLVQQIEA
                     IATKLYGASAIDLESQARKDLERVEKLGLGHAPVCMAKTHLSLSHDPGLRNRPTGFIL
                     PIRGLVPSAGAEFVVALCG"
BASE COUNT         1860 a         3635 c         3521 g         1837 t
ORIGIN      
        1 atggcaccgc cagcgtcgtg cggtccaccg actcgtattg accgagcatc aggttgtgag
       61 cgtagttggc ctgctggcac acgtactcga agagctcggt gccgtcctcc catcgaagcg
      121 agaggccgct cgtccacgga gcggtgtacg cgcccggatc gtcgatggtc agctcgtagt
      181 tgatcgtcaa cgagtcggcg cgggtgaact tctcgagcgt tcgcagcttc tcggtggtcg
      241 gcaggccgcg tcgatcgatc cagaaccctt cgttgaagcc cgtggtctcg acgagcagcg
      301 tgtcgccctc ccaccagccg atcgagtgtc cgtagtaggt gggcgtgagg ttctccggat
      361 gcgtcctgcc gtccatgaag atggtgcggt aggtgtgcgg tccgccgatg tcgaagatgt
      421 agatgcgctg cagctcgcgc agctcgacga tctccacgcc gtagggtgtg atgaattgcc
      481 ggatgccgcc cgacggcttg cagcgtgtgt gcggctcgag ttggtgccgg ccgcggtctg
      541 cgtagacctc ccgcgcccac ggctggaagg ggatctgacg ctccgcgttg ggcgccgcgc
      601 cgccgccacc acccggctgc cagacaccct tctgggttgg tgtcgccgag gtgaggagca
      661 cgcgcccgtc aggaccgcgt ggcgcgggct gcgcgggggg gcctgccgcg cgccctcgtc
      721 caccttgacg tcctgctgcg ccctcgcgcc cttcgggaac acccacctgc acgctcggac
      781 gctgcggcgg ctgcccgtcc tgctggccga gcagcgtgcc ggcgagcact ggcgaaaaga
      841 acaacgccgc ggcaccacac agcgcgagag tgtgtcgcat cgaactcccc tggagcgagg
      901 tagcggttgt ccggaactag ggattcgatc cctggctcga gacggcgtcg agcgtctctc
      961 cgggtcgccc gccagtggcg agcagcgtga ccgatctggc gttgaccttc ttgctgccgt
     1021 tcttcgccag cgagccgctc accttgacct cttcgccgat cttcagcgtc tcgcgccgcc
     1081 agccgcgccg ctggagtcca tggggcggac ccatctcggc gccccagttg ctcacttcgc
     1141 cggtcttctc gtccgtcacg ttgatgtaga accacacgtg cggattggtc cactcgacct
     1201 tcgtgacccg gccggtgagg gtaatcggct tgttggcgtc gaactccgcc gcgaacgagt
     1261 gatgcgccac cgcacttccc gccgcgaaca tgaggccagc tgcaaccaca ccacccagaa
     1321 tgtacgctct catctgattt cctccaggct gatatatcag agcctgccta cggcctattc
     1381 tcctgacaca gatgtctcgg catgtcctcg ctgctcgtga cccagcgcag cgtccacccg
     1441 ctcgaccacg gccgcgtgta tgccccagcg tcctcgatcg tcacctcgta cttcagcgtg
     1501 tcgaagttct cgcgcgtgaa ccgctcgacc agccgcagtt gctcggtatg tggcaatccg
     1561 ccgttggtga accagaagtc ctcgttgaac ccccgggtgt ccacgaccag cgtgttcccc
     1621 tcccattttc ccaccgcccg gccgtaatag agcggattat cgtcgtcgcc cccgacctgt
     1681 ccggcgtggc cccggccgtc caggtaaatg atacggaaat tgttgtttcc cccgccaatc
     1741 aggacgaaaa tccgctgccg ctcgcgatct tccacgagtt gcacgccgtt ggacagctga
     1801 aactgacgtg gcccccccgg cggcttgcag ttgagatacg tcggatcgtc ctgcagaaaa
     1861 cgacgctggc gaagctcgta cagcgcgaga gcccagggct gcatcggcgc cacccggcgg
     1921 gcgtccgcga cattcctcag gaggccgtac tggtccattt ggacgttcga gccgttttca
     1981 gcgagcaccg tcgaactcgg aaatgcccag tagccctgga ccccgccggg aatcggtccg
     2041 agccggggct gaccgtcagc ccatttcggc gtcggcctgt tctgaaggag cgccggcggc
     2101 gaaggcggtg cgacgttgag tacctgtctg ccggtaccgg tcatcacgat ccggttggcc
     2161 cagacctgac ggctgccgtt gcgagcggcg attccggtta cggtaatcga gttgccaggc
     2221 tggaccgact cactgttcca tccgcccgtc tgcagatcga tggggctctg gagctcgacg
     2281 gcccagttgt cggcgtcgtt gccgcgccgc acgttcatgt agatgtgcac gtgcgggttc
     2341 cgccagtcga cgagcgtgac aacgccggtg agcgtgagcg gctttgtgtc gtcgaacttg
     2401 gcggagatcg gatggtgagc agcgccagac gccgctccga gggtgagcgc gagcacgacc
     2461 gctatcgcca atgcgacgac gaacctcatg ggaactcctt actgccggct gatcttcagc
     2521 tgtccgctct cgcgctggtt cttccacgtc ccgacgatcg cgcggttgtg gaacgagagg
     2581 ttttcgatct ttccgtcgat cacgtagttg agcaccgcgc ccgacttttc cttgatgtcg
     2641 gcttcgaggt gcacgaccca gccgtcggga ttgagcgtcg cgttcttgat cggggcgtcg
     2701 ttcacacccg ggttgatcgt cccgctgatc gtcttgccgt cccagttgag cacgaggacg
     2761 atgtcgttgc tgtgagactt ggcaggcccc caggtgccca gccaggatcc cttcaacgga
     2821 tgcccttcct gagcggtgac ggacacgccg ccggacaggc acgccaccaa cagtgtcagc
     2881 acgatagtca acgctcgcat gggttcggct ccctccgtgt gtgcgcaaag cgtaagctat
     2941 tcacgaaacc gtgttcaagc gttaaaggca tcccgttgcg cggcattgga tctgtccgga
     3001 acaacgcatc ccggggcggc cctgtctttg cacccaccca gccgtatggc ccgccattcg
     3061 gcctgtctgc ggctgacgct gctgttcgtc gtggcgtggg tgtgtgtttc cccgcctgct
     3121 cgcgtggctg ccgaggcgcc gccggcagcc ggtggagaag tccgggagat ggtccgactc
     3181 accaacgccg aacggagccg ggctggccgg gcgccgctgg ccgccagccc acgcttgatg
     3241 aaggcggcgc aactgcatgc cgagcagatg gtggcacgtg gccagatggc ccacgatctg
     3301 cctggggcgc gctacccgca gacggaggat cgcctcgcgg ctgcccagta ccggtggctg
     3361 tcgtacgcgg agaacgtcgc ctacggacag ccggacccag tgtcggcgct cgattcctgg
     3421 atggactcgc cgggtcaccg ccgcaacatt ctcaacgcct cgatgacgga gctgggcacc
     3481 ggtttcgccc gcgaccgaag cggacggccg tattacgtcc aggtgttcgg caggcctcga
     3541 tcgcgataag ggttgcgcga ccgcgtagag cgggggcttc agcccccgcg tgctagtatt
     3601 cccacgcgtt ttcccttcgc ggattcccat tccgaacgtc ttcggcgctt cctggcccaa
     3661 aggagccaaa ccatggtcat cacacgtgtg ggacccctgt cggtcgcgaa ggttgccgga
     3721 ctgctgtacg gcatcatcgg attgctgttc ggcgtcttca tttcgctctt cgccatggcc
     3781 gggtttgccg cgggcgcgaa tgactcgccc tttcgcgcag tcttcggcgt cggcgcggtg
     3841 atcctgcttc cgatcttcta cggctgcctc ggttttgtca tgacgctcat catgtcgtgg
     3901 ctcttcaaca tcgtcgtcgg catcaccgga ggcgtggagg tggacgcgcg ctgacgctga
     3961 gaccgcgttc ggcgggattg accacgagcg tgttcaccag gaagcgtgtg ccgtcccgcg
     4021 tcacatcgaa ggcgtgatag gcgccgttcg gaaaggagag ctggaacagt tcctcgactc
     4081 gcgtgaccga gaactcagcc cctgatgccg acaccgacgc cgccatcagc atgcgattgg
     4141 gcgcgacgta gcagatctcg ccatcgcgcc gccacctcgc ctggaatcca ccttcggcgg
     4201 atatctgcca gcgcctttcg gtcggcggga acgccgacac gaacacctcg gcgcgtccgg
     4261 acacggtgac gctgaaggcg acccaacggc catccggaga aaacgccgcc cagttttccg
     4321 cctcctccgt gcgaaggaac ggagaggtcc gactcgagcc gtcgagcgac agcacggaga
     4381 tgtcgttgcc ggtgcgctcg ctgttggtga cgtagaggac ggagcgccca tccggcgacc
     4441 agctcagcgg ccatatgccg tcggctcccg caagcagttc gttgcgtacg cccgatcctt
     4501 tcgcgggcct ctcgaacagt ccgaggcggc cgggtgcgaa cgagttgaac agcatgcgcg
     4561 cgccgtccgg cgaccagatc gaccagttct catcggccgc gtccgtggtg aaccgcgtcc
     4621 ggctgtccga gtccacctcg tatatccaga tgtcgcgcga cacgctggac cgatcggtca
     4681 ccgcgacggc cacgcgtctg ccatccggcg agagctccag gttgccgtgg tcggcgacgg
     4741 ggccgagcgc accgagacgc tggccgtcgc gatcgaacca gagcagccgc gacagcacca
     4801 cggggcgcgt cgactgcgcg gcgagccacg gggcgagtgt cgtggcgaga agcacgccga
     4861 gaagtgtccg gcgcatgccg gcgagatcat agcgcgctcc cgaatcccgg ctcccgaccg
     4921 tttcgcaggg cgaatgactt ccgcgcagcc gttgcagtac actgaaaggc tgtttgcgcc
     4981 caaaactgct acagaatccc cacaaacctt agacaagcgg cccgtgcggt gatcgttcac
     5041 attaatcaca tttcgcaaag caagaagccg aagagcagcc agcgttcccc ccagagcgag
     5101 atggcgggtc tggtcaccgg gtcgctgatg tcgctcgtcg aagacggcgt gctcgacgcc
     5161 cggctcgcgc cgtcgttgag gattcatctc gactggatcc agtacaagac caactttcgc
     5221 gaccccgtca tggtccgccg caccacgaac gcgcaggggc agcagatgcc cctcgcggag
     5281 atcgcgatcg accttcggca ggccgacgcc ggccggctgc aggacgatct cgcgcgcgcg
     5341 gtgaagagcc tcggcgcggg gaccgagtcc aacaccgaga gtcacctctt cctcgacgac
     5401 ttccgcccct accgcgactg cctgatgtgg agcttcaacc ggctgttctg gcggcacctc
     5461 ggcgactggg aagccgcctc ggggaagagc ttcgaggcgg cgctgcccgg cgggtcttcc
     5521 gacgccaatc acccgcaggc ggtgtcggac tcggtggccg acttctggac gctgcttcgc
     5581 gacctcgagg cgcgcggaaa gctcccaccg gagatcttcg gattggagat cggcgtgggc
     5641 tcgggcgcgc gcgcggcggc gtggctggac cgcttcaagg cgctcgacga gcagtgcggc
     5701 acctcgttct actcccggat gcacttcatc ctgggcgact actcgccgac gagcctcgaa
     5761 cgcgcgctgg cggctgtcgc ccaccacgga agccacgcca gcggcgtccc gctcgacgcg
     5821 ctgaacccgt tcaagtcgct cggtccctac cgcttcaaga tcatgtacgt gcacctgacc
     5881 aacgtgtacg acaacctccc gttcgacgat ctggttcgcc gcgacgcgaa gttgtacctg
     5941 gtcgaagtgc gtccgtactt gaacggtccg atggtggatc gcctcacggc cgatttcggc
     6001 gtcacgcgcg ccgacctccc cggcctggtg accacgcttc tcgacgccgg accgcacgcc
     6061 gtgtttcccg gcgagaaagg cgtgctgttc tggcgccggc tctgggacac gttcaagctg
     6121 gaggagcggc tccgggtgct cgacgatcgc gaggaggatc acgtgccgcc gggcctgagc
     6181 cgcacacacc tcgaggatct gctggccgag gcgcccgacg acgtgcggtt ccacatttcg
     6241 agaggcgcgg gggagagctt cgccaacacg ctaccgctgc tgcacccgcg ggggttcctc
     6301 caggtgcagg acatcttcgt cagcgagatg gacgattacc ggaagggctt caaggggccg
     6361 ggcaagctgg atgggtcgct cgtcacctgg gtgaacggcg cgctgctgag ggccgtcggc
     6421 gcgcgcgccg gttacgacgt tcacttcgcg ccgttcccgt accgccccgg gacgaagacg
     6481 agcattctct acacgacgca acgtgactaa ccgcgaactc ccaactccca ggaaaacggg
     6541 gtcgggagtc attttcctgt cgcaagccgg cccgcggaaa atgactcccg accccatttc
     6601 tcgtcagcca cgggagcgtg tcctcaagaa atgactcccg accccaagga taataaagac
     6661 atgacgccgc cactgcccat cctcccgacg accgtcatcg gcagctactc gatgccggaa
     6721 tggctcgagc gggcgaagaa cgagtacctt tcgcgccgcc tgagccggcg cgacctggac
     6781 gagatgcacg acgcggtccg caaggccgcg atcaaggacc aggaaagcgc cggcatcgac
     6841 atcgtctcgg acggcgaggc gcagcgcgac aacatgatcg actacttcac cgagcggatg
     6901 cccggcgtcc aggtcgatct cggatcgaag cgcttttact acgacttcta cgaaagcgtg
     6961 gtgcgctcga agctggcgac cggcacgctc ggcctggcgg aggaggcgcg gttcctggcg
     7021 cgcttctcgg agctgggatc gaagatctcg atctccggcc cgcacacgct ggtcaagcgc
     7081 atccagaaca agcactacgc caccgaggag gcctttgcgc tcgacctcgg acacgtgctg
     7141 aacctcgagc tcaaggaact ggtcaaggcg ggcgccacgc gcctgcagat cgacgaaccg
     7201 tattactcgg gctttcccga ggatctcccg tgggcgctca aggccatcaa cgcaatggtg
     7261 gacggcgtca acgcgcacgt caccctgcac atctgctacg ggaaccgcta cggcaagccg
     7321 tcgttcgagg gcagctacga gttcctcttc ccgacaatca tggaggccag ggtgcaggcg
     7381 atctccatgg agtttgcccg ccgcggcgaa gacgacatca agctattcaa gacggttccg
     7441 ttccaggtgg gcattggcgt gatcgacgtc aagaccaacg aggtcgagac ggcggcgttt
     7501 gtcgccgatc ggatccgtcg cgccctcgaa gtcattcccg ccgagcggct gatcatcacc
     7561 cccgactgcg gtctcgtgcg gctcccgcgc gaggtcgcct tcgccaagct cagcgccatg
     7621 gtggaaggca cgcggatcgt gcgaaaggaa ctgggtaagt gaggccactt gccgagcgcg
     7681 tgaagtccgg ccagttcgtc tacatgacgg aactggtggc gtcgggcctc aagcgcgagg
     7741 cgcaggtcct cgagatcgcc tcgaatctgg ccaggattcc cgagatcgcc gccggcagca
     7801 tcaccagcta cgcgggtggg cagctcggcc aggactcgat ccgcgtcggc acggccgtgc
     7861 gcgcccgcgg actcgtgccg aacgtgcacc tgacgtgcgt cgggcaggat cgcacgacga
     7921 tcgaccggac gctcgcggcg ctccgcgcgc tcgagatgca caacatcttc tcgatcagcg
     7981 gcgactggcc gaaggcggcg atgcggacgg ccgtcttcga catcgactcg gtgcagctcg
     8041 ccgcgcacat cgccgacctg agggcccgca ccggcacccc gttcttcatc tcctgcgccg
     8101 tgtcgccctt caagtatcag cgcgaggacc tgctctatca gtacttgaag ctcgagaaga
     8161 agatcgccgc cggcgccgac atggcgatta cccaggtcgg ctgggacgcc accaagttcg
     8221 tcgagctgaa gaagtacctc gatgaacgcg gcatcaggac gccggtgctc ggcaacgtgt
     8281 acgtcctgag ccggcgtgcc gccgagcgca tgaacacggg caacccgccg ggatgctggg
     8341 ccagcgacga gctggtcgcc gtgctgcgga aggagtcgga ggcgcccgat ggcggcctcc
     8401 aggcgcgcct cgagcgtgct gcccgatcgg tcgcggtcct caagggcatc gggtatgccg
     8461 gcgcctacat cggcgggacg cacgacgcga agcacgtgac gcacatcatc cgccgcgccg
     8521 aggagctcgc gccgaaatgg cagcagcttt tcgaggagct gcggttcggc aagaaggacg
     8581 gcttctacct gtacaagcgg gatccgcatc cgaagccgcg ccgcacgttc ctgcccacgg
     8641 tgatggacgc cgccggcaag acgctgccct tcgtaccgtg gtcgaagacg gtcagggact
     8701 cgctgatgcg ccgcgccctc cagggcacgt tcagatggct cgatcgtcat ccgcgcctga
     8761 gccactggtt cgagcgcttc gagtactact cgaagaaaga agtgtttggc tgccagaact
     8821 gcggcaactg cgtgctgggg cacatggagt acgtctgccc gcagacctgc cccaagcaga
     8881 tgcgcaacgg accctgtgga ggcacgttcc acggcgcctg cgaggtcatc gatcaggagt
     8941 gcatctgggt caaggtgatg gagcgcgccg aggcctcgaa caccatcgac cagctcaagg
     9001 tgtacatccc gccgcccgat cgcacgctga cgggcacggc atcgtggctg aacttctatc
     9061 ttggccgcga cagccggccg gaccgaccga aggagcggtg ggagagcggc ggcccgggcc
     9121 ggaccctcgc catcgaggag gacgagaagc tgcagacggc cgggcctccg gccgccgaca
     9181 aggcgctcgc cgccaggacg gccaagacgc ccggaaaagt ggaagccccg gcaaaagcgg
     9241 aaaaagccac cgcatagagg acaccgttac acgatgaaat cagcgctcga gatcgcccac
     9301 gaagcacaac tccgtcccat cgccgacgtc gcggcctccg cgggcatcct gcccgacgaa
     9361 ctggagcagt tgggccggta tcgcggcaag gtacgtctgt cgattctcga caggctcgca
     9421 acgcgcccca acgccaagct ggtgatcgtc accgcgatca cgcccaccaa ggcaggcgag
     9481 ggcaagacga ccacgagcgt ggcgttgacg caagggctcg gcaagatcgg caagaaagtg
     9541 atgctgtgcc tacgcgagcc gtcgatgggc cctgtcttcg gcgtcaaggg cggcgggaca
     9601 ggcggtggct actcgcagat cggtccgatg gaggacatca acctgcactt caacggcgac
     9661 ttccatgccg tgaccgcggc gcacaacctg ctggctgccg ccgtggacgc ctcgatcttc
     9721 aacggcaacc cgctgcgcat cgatccgtca accgtgatct ggccgcgcac gctcgacatg
     9781 aacgatcgcg agctgcgcta cacggtggtc ggcctcgggg gcaagacgca cggcgtgccc
     9841 cgcgagaacc agttcatcat caccgccgcg tccgaggtca tggccgtctt cgcgctggcg
     9901 gggagcctcg cggatctgcg ccgtcgcctc ggccgcatcg tcgtcgcctc gaactacgag
     9961 ggggagccgg tcacggcgga gatgctcaag gtggccggcg cgatgacggt cgtcatgaag
    10021 gacgcgatca agccgaacct cgtgcagacg ctcgaggggc agccggcgct ggtgcatgcc
    10081 ggaccgttcg gcaatatcgc gcacgccgcc aactcgatcc tcgccgatcg catcgcgatc
    10141 aagtcggcgg actacgtggt gaccgaggca gggtttgcca gcgacctcgg cttccagaag
    10201 ttctgcgaca tcgtgtgccg cgtcggcggc ttcgcgccga gcgccgccgt gctggtgacg
    10261 acggtacggg cgaccaagtc gcacggcggc aagtccttca acgtgctcgc gaacgaggac
    10321 ctcgattcga tcgccaaagg cgccgacaac ctggccgcgc acgtgcagat cgtccgtcag
    10381 tacgggttgc cgtgcgtggt cgcgatcaac agcttcccga ccgacaccgc gcgcgagctg
    10441 gcgctcgtcc gcgagctggc gctcaaggcc ggggcagaga ccgtggtgac ccattccggg
    10501 ttcgtcgagg gcggggcagg cgccaccgat ctggccaagg cgatcgtgtc ggcgtgcgac
    10561 aagccgaaca ccttctcgca cctgacgccg gacggaacgc cgctcgtgca gcagatcgag
    10621 gcgattgcca cgaagctgta cggcgcctcg gcgatcgacc tggaatcgca ggcgcggaag
    10681 gatctcgagc gcgtcgaaaa gctcgggctg ggccacgcgc cggtgtgcat ggcgaagacg
    10741 cacctgtcgc tgtcgcacga cccggggctg cgcaaccggc cgacgggatt catcctgccc
    10801 atccggggcc tcgtgccgag cgccggcgcc gagttcgtcg tcgcgctctg cgg
//
LOCUS       DBNGSH010000830         3467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3493758, whole genome shotgun sequence.
ACCESSION   DBNGSH010000830 DBNGSH010000000
VERSION     DBNGSH010000830.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3493758"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2824)
                     /locus_tag="AC16CY_19110"
     CDS             complement(<1..2824)
                     /locus_tag="AC16CY_19110"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3355968.1"
                     /translation="MRVVPAAPVRLRQGYGETSPKRFARRRAARFDRVESFIKWWTLP
                     AALRRSVPIARIERLGLALSSVVVLLGVSLTYVGQTRSFETVRRDVAGGRVINLESVT
                     SGAALAPALRIFDQQAERTEVASAIVRRLRAPEAPALTHVGGLAALTIPASTVRRDAR
                     LAVLNERLRAHPAADAVPLLTSADIVAIKPGLIARSPEAYMRQVAIALAVVLAAFWIS
                     HIIRSLGHATGDPIVLPAIGLLTGIGFASMLALRDPLRDSVLVVPYAGGIAAGVALFT
                     AIAFFDFEAPRLRRAVLAPLTAATLLAAALLVFGSGPSGSDAKVNLLGVQPVEAIRLL
                     AVFSLAAYFARRWQFLREFSETVALTDRRFRVRVPRWKDVRPLAITIATLLTFFFLQK
                     DLGPALVLSCVFLGVYGIARARTALVLCGFAVLALGFGAGFVLGVPATVTRRVTMWLD
                     PWNNAMTGGDQVAHALWAFATGSVLGLGPGMGDPRLIPAGHTDLVLAAVGEELGFVGV
                     LVVIGLLGLLVWRMLRASLRAPGDYTSFLAAGLCLVIAVQALVIAAGVLGLLPLAGVV
                     TPFLSYGRSSMLSNFAAAGICCAIARRAGPVREPFARPIRTTGRALAAAALLVVTAAG
                     LVQVVRADGIASRGNVTRQADGGYRYQYNPRLVAAARQIERGTIYDRAGVPLATSRPE
                     ELQSFANELRTLGAPIPSGCDDGRSRCYPLAGLAFHVIGETEFQTNWAARNTSFVERE
                     FDARLKGYDDAPRTVLIRDPEGHTTRGVVRDLSDLLPLARHPGNAGHPAVRQVMEAPR
                     DVRLTLDARLQIRVARALEAYASKAAGRGAVVVLDATSGQVLASASYPWPDFEQLGNS
                     VRLPSTTLGPGKPDTTYAGVRLKPDTTYSERLLDRARYGLYSPGSTFKLITAAAALRS
                     DPAAERTTFQCSRLEDGRVGGRVRGAS"
     gene            complement(2830..>3467)
                     /locus_tag="AC16CY_19115"
     CDS             complement(2830..>3467)
                     /locus_tag="AC16CY_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355969.1"
                     /translation="MVDLADRAILDYRTELPTVNEVQWQRALECLGLARELAPGDRRV
                     AGKHAYVSAHLQRINAKTKEDVDRAIRLFRESARLDSRSPDPYLGLARVAAYVTQDPE
                     ALRQAILEAQNRGYVSGRRERAQVGDLHKVLGDRARADATRLNGASRLERLQRAAAEY
                     QGCIESFSELHYFNSDQNLTSCRRRLEQVEAEIERARPAPPDAGLVLQPAG"
BASE COUNT          593 a         1215 c         1212 g          447 t
ORIGIN      
        1 tggacgcgcc gcgcacgcgg ccgccaacgc ggccatcctc gagccggctg cactggaacg
       61 tggtgcgttc cgccgcggga tcgctccgga gcgccgcagc cgccgtaatc agcttgaagg
      121 ttgaccccgg cgaatacagt ccgtagcgcg cgcgatcgag cagccgctcg gagtacgtgg
      181 tgtccggctt tagccggacc ccggcgtacg tagtgtccgg ctttcctggc ccgagcgtag
      241 tcgagggcag ccggaccgaa ttccccagtt gctcgaaatc aggccacgga taactcgccg
      301 acgccaggac ctgtcccgac gtcgcgtcga gcacgacgac ggcgcctcgc cccgctgcct
      361 tggaggcata cgcctccaac gcgcgcgcga cgcggatctg cagccgcgcg tcgagcgtca
      421 gtcgcacgtc ccggggcgcc tccatgacct gtcgcacggc tggatgtccg gcattacctg
      481 gatgccgcgc gagcgggagc aggtcgctga ggtctctcac gacgccccgc gtcgtgtgcc
      541 cctccgggtc tcgaatcagc accgtcctcg gggcgtcgtc gtagcccttc aggcgcgcgt
      601 cgaactcgcg ctcgacgaac gacgtgttcc gcgcggccca gttggtctgg aactccgtct
      661 cgccgatgac gtgaaacgcc aggccggcca gcggatagca gcgggaccgc ccgtcgtcgc
      721 agcccgaagg tatcggcgcg ccgagcgtgc gcagctcgtt ggcaaacgac tgcagctcct
      781 cgggccggct cgtggcgagc ggcacgccgg cgcggtcgta gatcgtgccg cgctcgatct
      841 ggcgcgcggc ggcgaccaga cgcgggttgt actgatagcg gtatccgcca tccgcctgcc
      901 gggtgacgtt gccgcgcgag gcgatcccgt cggcgcgcac cacctggacg agcccggccg
      961 cggtgacgac gagcagcgcg gccgcggcga gagcccgtcc cgtcgtgcga atcggacggg
     1021 cgaagggttc ccgcaccgga cctgcccgcc gggcgatggc gcagcagatt ccggcggcgg
     1081 cgaagttgct cagcatcgac gagcggccgt aactgaggaa gggagtcacg acgccggcaa
     1141 gcggcagcag accgagcacg cccgctgcta tcacgagcgc ctggacggcg atcacgagac
     1201 acaggccggc ggcaagaaaa gacgtgtagt cgccgggggc acgcaacgag gcgcgcagca
     1261 tcctccacac gagcaagccg aggagcccga tcacgacgag aaccccgacg aagccaagtt
     1321 cctcgccgac cgccgccaga accagatcgg tgtgaccggc gggaatcagc cgcgggtcgc
     1381 ccatgccggg gccgaggccc agaacagacc cggtcgcgaa cgcccacaac gcgtgagcga
     1441 cctggtcgcc tcccgtcatc gcgttgttcc acgggtcgag ccacatggtg acgcgacgcg
     1501 tcaccgtcgc cggaacgccc agcacgaagc cggcgccgaa gccgagagcc agcaccgcga
     1561 acccgcacag gaccagcgcg gtgcgggctc gcgcgatccc gtagacaccg aggaacacgc
     1621 acgacaggac gagcgccggg ccgagatcct tctgcaggaa gaagaacgtc agcagcgtcg
     1681 cgatcgtgat cgccagcggc cgcacgtcct tccaccgggg cacgcgcacg cgaaagcgcc
     1741 tgtcggtgag ggcgacggtc tcggagaact cgcgcagaaa ctgccagcgt cgcgcgaagt
     1801 aggcggcgag cgagaacacg gccagcaaac ggatcgcttc gaccggctgc acgcccagca
     1861 ggttgacctt ggcgtcgctg cccgatggtc cgctgccgaa tacgagcagc gccgctgcca
     1921 gcagcgtggc ggcggtgagc ggcgccagaa ccgctcgccg caagcgcggc gcctcgaagt
     1981 cgaagaaggc aatcgcggtg aacagcgcga cgccggccgc gattccgccg gcatacggca
     2041 cgacgagaac ggagtcgcgc agcggatcgc gcagggcgag catcgaggcg aagccgattc
     2101 cggtgagcag cccgatcgcg ggaaggacga tcgggtcgcc cgtggcatgc cccagcgacc
     2161 ggatgatgtg cgagatccag aacgcagcaa ggacgacggc caaagcgatt gcgacctgac
     2221 gcatgtaggc ctcgggcgac ctcgcgatga ggcctggctt gatggccacg atgtcggcag
     2281 acgtgagcag gggcaccgca tcggccgccg ggtgcgcccg cagacgctcg ttcaagaccg
     2341 caagacgcgc gtcacgacgg acggtgctcg cggggatcgt caacgcggca aggccaccga
     2401 cgtgagtcaa cgccggcgcc tccggcgcgc ggagccgccg cacgatggcg gaagcgacct
     2461 ccgttcgttc ggcctgctgg tcgaagatgc gaagcgccgg cgcgagcgcc gcgccggagg
     2521 tgaccgactc caggttgatc acgcgcccgc cagccacgtc gcggcgcacc gtctcgaagc
     2581 tcctcgtctg ccccacgtag gtcagcgaga cgccgagcag cacgacgacc gacgagagcg
     2641 cgagccccag gcgctcgatg cgcgcgatcg gcactgatcg ccgaagcgcg gcgggcagcg
     2701 tccaccactt gatgaaggac tcgacgcgat cgaatcgcgc ggcccgcctt cgcgcaaagc
     2761 gcttcggcga ggtctcgccg tagccttggc ggaggcggac cggcgcagca ggaacgacgc
     2821 gcatcgtgct caaccggcgg gctgcagtac caagccggcg tcgggcggcg ccggacgagc
     2881 gcgttcgatc tcggcttcga cctgctcgag acgccgacgg cacgaggtca ggttctggtc
     2941 cgagttgaag tagtgcagct cgctgaagga ttcgatgcag ccctggtact cggcagccgc
     3001 gcgttgcagc cgctcgagcc gcgacgcgcc gttcagccgc gtggcgtcgg cgcgggcgcg
     3061 atccccgagc accttgtgca ggtcgccgac ctgcgcccgt tcacgccggc cggagacgta
     3121 acctctgttc tgggcctcga ggatcgcctg ccgcagcgcc tcggggtcct gcgtcacata
     3181 cgctgccacg cgagccaggc cgagatacgg atccggcgat cggctgtcga gccgcgccga
     3241 ttcacggaac aagcggatcg cccggtcgac gtcttctttc gtcttcgcgt tgatccgctg
     3301 gagatgcgct gacacgtacg cgtgcttgcc ggctacccgg cggtcgccgg gcgccagctc
     3361 gcgcgccagc ccgaggcact cgagcgcgcg ctgccattgc acttcgttga ccgtcggcag
     3421 ttcggtcctg tagtcgagaa tcgcgcggtc cgccagatcg accatcc
//
LOCUS       DBNGSH010000831         1825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3324331, whole genome shotgun sequence.
ACCESSION   DBNGSH010000831 DBNGSH010000000
VERSION     DBNGSH010000831.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3324331"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="AC16CY_19120"
     CDS             complement(<1..504)
                     /locus_tag="AC16CY_19120"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3355970.1"
                     /translation="MSQLRLAASGLWVLSLVGCGAPPATPVTDRAAPAGAAAPEGAES
                     GTATLPAGFPERPFVRVQFDTLAWRPTEGNTLGVETAIIEGDPSKPGYYLTINHFPAG
                     VMSRPHYHPDERYIVVLRGTWYTDEGEIFRPNQTVPLKPGDFMRHPRNGPHYDGALDE
                     DTWVAIGG"
     gene            complement(530..1000)
                     /locus_tag="AC16CY_19125"
     CDS             complement(530..1000)
                     /locus_tag="AC16CY_19125"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3355971.1"
                     /translation="MRTAFGVPVALVMLLVTATAAMAHHSFAMFDRTVERVVTATVVR
                     WAFNPPHSWLYLNVKNPDGSVTLWAFEGSAPPSLLTRGITGSTFEPGDTVTISYCPLR
                     DGRPGGGLGWARLANGTFVNPSDGGCDGSVKAIEKWKGWLAAGYTSSTDAQKPK"
     gene            complement(1004..>1825)
                     /locus_tag="AC16CY_19130"
     CDS             complement(1004..>1825)
                     /locus_tag="AC16CY_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355972.1"
                     /translation="AKGGTKHTLTSVPRWDGLWVTAGNTHMNAFIDPPGFAGKVRAGV
                     LTPPYEKAYRERWRQQQELGEVQYDRLTHCEPPGYPRWLLEPYSHEFINLPRESYLIN
                     DYGPGIRRVYIGQEHKNVYGTHSWYGDTIGFWDGDRLVTHTKFLLPGDFTRWSPMTSN
                     QFESVETWELKRYPGGIERLEVQATFYDRHALARPVSAVYAFRRGTELEAAGHRVQHW
                     ECEGSSNDVFDKGTTTSRLPGEAGFKDVRGTTLFPDLPGQSRDPIYNTTLPGGKE"
BASE COUNT          284 a          624 c          589 g          328 t
ORIGIN      
        1 gccgccaatg gccacccagg tgtcctcgtc cagcgcgcca tcgtaatgtg ggccgtttct
       61 ggggtgccgc atgaagtctc caggcttcag cggaacggtc tgattcggac ggaagatctc
      121 tccttcgtcg gtgtaccacg tgcctctgag cacgacgatg tagcgctcgt cagggtgata
      181 gtgcgggcga ctcatgacgc cggcgggaaa gtgattgatc gtcaggtagt agccgggctt
      241 cgacggatcg ccttcgatga tcgcggtctc aacgccgagc gtattgcctt ccgtgggccg
      301 ccacgccagc gtgtcgaact ggacgcgtac gaacggccgt tcgggaaatc ctgccggcag
      361 tgtcgccgtt ccggactcgg caccttcggg tgcggcggcg cccgcgggcg ccgccctgtc
      421 agtgactggc gtcgctggcg gtgcgccgca gccaaccagc gaaagcaccc atagacccga
      481 cgccgccagt cgcagctgag acatgctcga acgactcctc tccgccgcac tatttcggtt
      541 tctgcgcgtc ggtgctcgac gtgtagccgg cggcgagcca ccccttccac ttctcgatgg
      601 ccttgacgct gccgtcgcag ccgccgtcgg aggggttcac gaacgtgccg ttggccagac
      661 gcgcccaccc gaggcctccc cctggtcgcc cgtctctcaa gggacagtac gagatcgtaa
      721 ccgtgtcgcc gggctcgaac gtgctgcccg tgatgccgcg cgtgagcagc gacggcggcg
      781 ccgagccctc gaaggcccac agcgtgacgc tgccgtccgg gttcttcacg ttcaggtaga
      841 gccacgaatg cggcggattg aacgcccagc gcacgaccgt ggccgtcacc accctctcga
      901 ccgtgcgatc gaacatcgcg aacgaatggt gggccatcgc ggccgtcgcc gtcaccagca
      961 acatgaccag ggcgacaggc acgccgaacg ccgtcctcat ctcctactcc ttcccgcccg
     1021 gaagcgtggt gttgtagatc ggatcgcgcg actgacctgg cagatccgga aacaacgtcg
     1081 tgcctcgcac gtccttgaat cccgcctcgc ccggcagcct cgaggtcgtc gtgcccttgt
     1141 cgaagacgtc gttgctgctt ccctcgcact cccagtgctg gacacggtgg ccggcggcct
     1201 cgagctcggt cccgcggcgg aacgcgtaga cggcgctcac gggcctggcc agggcgtggc
     1261 gatcgtagaa ggtggcctgc acctcgagcc gctcgatgcc gcccgggtac cgcttgagct
     1321 cccacgtctc gaccgactcg aactgattgc tcgtcatcgg cgaccagcgc gtgaaatcgc
     1381 cgggcagcag gaatttcgtg tgcgtcacga gcctgtcgcc gtcccagaat ccgatcgtgt
     1441 cgccatacca cgagtgcgtg ccgtacacgt tcttgtgctc ctggccgatg tagacacgcc
     1501 ggatccctgg cccgtagtcg ttgatcaggt acgactcgcg cggaaggttg atgaactcgt
     1561 gcgagtaggg ctcgaggagc caccgcggat agccgggcgg ctcgcagtgc gtcaggcgat
     1621 cgtactggac ctcgccgagc tcctgctgct ggcgccagcg ctccctgtag gccttttcat
     1681 atggcggcgt gagcacgcca gcgcgaacct tgcccgcaaa cccgggcggg tcgatgaagg
     1741 cgttcatgtg tgtgttgccg gcggtcaccc acaggccgtc ccagcgcggc acggacgtga
     1801 gcgtgtgctt cgtgcccccc ttggc
//
LOCUS       DBNGSH010000832         4702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3494219, whole genome shotgun sequence.
ACCESSION   DBNGSH010000832 DBNGSH010000000
VERSION     DBNGSH010000832.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3494219"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="AC16CY_19135"
     CDS             complement(<1..1220)
                     /locus_tag="AC16CY_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR3355973.1"
                     /translation="MATAVSEVHAYELAKQAGRVPYKVADLGLAEFGRKELRLAEGEM
                     PGLMAIRQRYQGKKPLEGARIMGSLHMTIQTGVLIETLALLGADVRWVSCNIFSTQDH
                     AAAAVVVGRPESGGTVQNPKGVPVFAWKGETLDEYWWCTVEALVWPDGKGPSLIVDDG
                     GDSTLWVHKAAEFEKAGKVPAFNPDKDPEEWGVILKFLNEELSKNPGKWTAIAKGIRG
                     VSEETTTGVHRLYEMQKKGTLMFPGINVNDSVTKSKFDNIYGCRHSVIDGLNRATDVM
                     LGGKVAVVFGFGEVGKGCAEALRGQGCRVVVTEIDPICALQAAMQGYEVKTMEDVVST
                     ADIFVTATGNFNIVTAEHMSKMKDKAIVANIGHFDNEIDMAGLAKVKGVKRINIKPQY
                     DEWVFPDGHSVMILA"
     gene            complement(1306..2241)
                     /locus_tag="AC16CY_19140"
     CDS             complement(1306..2241)
                     /locus_tag="AC16CY_19140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3355974.1"
                     /translation="MVAPILDHMTALSDPTRCRMLLLLEKHELTVSELCAVLQLPQST
                     VSRHLKTLGDDDWVTSRRDGTSRFYTMPVDDLDSGAARLWPLIREQVLSTRGAEQDER
                     RLRGVLGRRRAKSEEFFASAAGGWDRLRGELFGDEFFLWAVLALIDSTLTVGDLGCGT
                     GQLTETVAPYVRRVISVDGSRDMLEAARQRLSSATNVDLRRGELESLPIESGELDVAM
                     LSLVLHYSPEPPRALSEVGRVIRPGGRILVVDMLPHDREEYQQQMGHVWLGFSETQIT
                     RLLTGAGFGDVRVRMLPVDPDAKGPALFAAMATKQ"
     gene            complement(2350..3372)
                     /locus_tag="AC16CY_19145"
     CDS             complement(2350..3372)
                     /locus_tag="AC16CY_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355975.1"
                     /translation="MRSSRVVRVALALVLVSLTYAPGNSQQPARPEGRGSAWVVPRTA
                     DGRPDLQGVWENNSATPLERPRQLADKPRLSDKELESFKLRAARLFGPEQDAIFGDGL
                     YLALLADATPPGLGATGTYSQNWLPDRYFEHRTSLIVDPADGKLPPLTPAGAKLRAAA
                     AGRFGRVGASAQDLSLQDRCIHYGFPDLFAAYMSVYRIVQSPEFVAIQMEKIHDARIV
                     PLDGRPHASSALRQFLGDSRGRWEGDTLVVETTNFHPKGNPMGGYSVLSDENLRLIER
                     FRRVADDTLEYTFTVDNPTMWTKPWTAVINWKRSRGELHEYACHEGNYSLRGMLSAAR
                     AEEAGR"
     gene            complement(3394..>4702)
                     /locus_tag="AC16CY_19150"
     CDS             complement(3394..>4702)
                     /locus_tag="AC16CY_19150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR3355976.1"
                     /translation="PHDSYSALTTADMTPPVVTLAAGDRVTLSYGQYRALLETNRNQA
                     DRVAAYRAFHGVYAGNRNTYAALYNGVLQRDWFHARARGYATTLDAALHGNDIPPSVV
                     ETLIAVTKEGIGPLQRYHRLRRRVLGLDRYHLADVFIPLVEHDRRYGYDQVASWIVDS
                     VAPLGAGYQQGVRDAFAGRWIDVFENMGKRSGAYSAPVYGAHPYMLLNYNETLDAVFT
                     LAHEMGHSMHTLLAHQAQPFVYAGYTIFVAEVPSTLSEALFLDYMLERADSGEQRAVL
                     LQHAIDSIASTFYTQVMFADFELQAHRLVEQDRPITAEVLSGIYATLLREYYGDVIDE
                     EPLSSITWARIPHFFSTPYYVYQYATCFASTARLMQDLRDGDSSTRAGAVERYLTLLR
                     AGGSDYPMNLLARAGVDLRQPDTVRAVATELEKLVSSLEGELG"
BASE COUNT          780 a         1593 c         1490 g          839 t
ORIGIN      
        1 gccaggatca tcacgctgtg gccgtccggg aagacccact cgtcgtactg cggcttgatg
       61 ttgatccgct tgacgccctt caccttggca aggccggcca tgtcgatctc gttgtcgaag
      121 tggccgatgt tggcgacgat ggccttgtcc ttcatcttcg acatgtgctc ggcggtgacg
      181 atgttgaagt tgccggtcgc ggtgacgaag atgtcggccg tgctgaccac gtcctccatc
      241 gtcttcacct cgtagccctg catcgccgcc tgcagcgcgc agatcggatc gatctcggtg
      301 acgaccacgc ggcagccctg accgcgcaat gcctcggcgc agcccttgcc cacttcgccg
      361 aagccgaaca ccacggcaac cttgccgccg agcatcacgt cggtggcgcg attcagaccg
      421 tcaatcacgg agtgacggca gccgtagatg ttgtcgaact tgctcttggt caccgagtcg
      481 ttgacgttga tgcccgggaa catcagcgtg cccttcttct gcatctcata cagacgatgc
      541 acgccggtgg tcgtctcttc gctgacgccg cggatgccct tggcgatcgc ggtccacttg
      601 ccggggttct tgctcagctc ctcgttgagg aacttgagga tgacgcccca ctcctcggga
      661 tccttgtcgg gattgaacgc gggcaccttg ccggccttct cgaactcggc ggccttgtgc
      721 acccacagcg tcgagtcccc gccgtcgtca acgatgagtg acggcccctt gccgtcgggc
      781 cacaccagcg cctcgaccgt gcaccaccag tactcgtcga gcgtctcgcc cttccacgca
      841 aacacgggca cgcccttcgg gttctgcacg gtaccgcccg actccggccg tccgacgacg
      901 acggccgccg cggcgtggtc ctgcgtcgag aagatgttgc acgacaccca gcgcacatcc
      961 gccccgagca gcgcgagcgt ctcgatgagc acgccggtct gaatcgtcat gtgcaggctg
     1021 cccatgatcc gggcgccttc gagcggcttc ttgccctggt agcgctggcg aatcgccatc
     1081 aacccgggca tttcgccctc ggcgagcctg agctccttgc ggccgaactc cgccagcccg
     1141 agatctgcga ccttgtacgg aacacggccc gcctgcttcg cgagctcgta tgcgtggact
     1201 tcactgactg ctgttgccat tgctcatctc ccacgcggtg gctgaagcca ccgctctacc
     1261 ctgtccctgc ccatgtagag cgcacgcctc agcgtgcgcg atcacctact gtttcgttgc
     1321 cattgccgcg aacagcgccg gtcccttcgc gtcaggatcg accggcagca tccgcacgcg
     1381 cacgtcgccg aagccggcgc ccgtcagcaa gcgcgtgatc tgcgtctccg aaaacccgag
     1441 ccacacgtgg cccatctgct gctgatactc ctcgcggtcg tgcggcagca tgtcgacgac
     1501 gaggatccgg ccgcccggcc tgatcacgcg gccgacttcc gacagcgcac gaggcggctc
     1561 cggcgaatag tgcaggacga gcgacagcat tgccacgtcc agctcgcccg attcaatcgg
     1621 caggctttcg agctcgcccc gcctgaggtc cacgttggtc gcactgctca atcgctgcct
     1681 ggcagcctcg agcatgtccc gcgaaccgtc aaccgatatc acgcgccgga cgtatggcgc
     1741 gactgtttcg gtgagctgcc cggtgccgca gccaagatcc ccgaccgtga gagtcgaatc
     1801 gatcaacgcg agcacggccc agagaaagaa ctcgtcgccg aacaactccc cgcgcagccg
     1861 atcccagccg cctgccgccg acgcgaagaa ctcctccgac ttggctcgcc gccggccgag
     1921 cacgccgcga agacgccgtt cgtcctgctc cgcgcctctg gtggagagca cctgctcccg
     1981 aatcaacggc cacagccgcg cggcgccgct atcgaggtcg tccaccggca tggtgtagaa
     2041 gcggctggtg ccgtcgcgcc gcgacgtcac ccagtcgtcg tcgcccagcg tcttgaggtg
     2101 gcggctgacc gtggactgcg gcaattggag aaccgcacac aattcggaga ccgtgagctc
     2161 gtgcttttcg agcaggagca gcatacggca gcgcgtcggg tccgacagcg ccgtcatgtg
     2221 gtccaggatg ggtgcgacca tatatccgta aatccggatt aaatgatacg catgaccacc
     2281 agcctgcgtc aagttcgcgg gtcaagccct gtttctggcc agaattggcc tctcctggcc
     2341 tgccccgcgc tatcgtccgg cttcctccgc ccgggcggct gacaacatcc cccggaggct
     2401 gtagttgccc tcgtggcagg cgtattcgtg cagttcgccg cgcgagcgct tccagttgat
     2461 gacggccgtc cacggtttcg tccacatcgt cgggttgtcc acggtgaacg tgtactcgag
     2521 ggtgtcgtcg gcgacgcgcc tgaagcgctc gatgaggcgc aggttctcgt ccgagagcac
     2581 ggagtaaccg cccatcggat tgcctttggg gtggaagttc gtggtctcga ccaccaaggt
     2641 atctccctcc cagcggccgc gcgaatcacc gaggaactgg cgcagcgcgg acgaggcgtg
     2701 cggccggccg tccagcggga cgatgcgcgc gtcgtggatc ttttccatct gtatcgccac
     2761 gaattccggc gactgcacga tccggtagac gctcatatac gccgcgaata ggtccggaaa
     2821 cccgtagtga atgcagcgat cctggagtga cagatcctgc gccgaggcgc cgactcgtcc
     2881 gaaccgtccc gcggccgccg cacgcagctt ggcaccggct ggcgtcagtg gcggcagctt
     2941 cccgtctgcc ggatccacga tcaaggaggt tcggtgctcg aagtaccgat cgggcagcca
     3001 gttctggctg tatgtcccgg tcgcgccaag tcccggagga gtcgcgtcgg ccagcaaggc
     3061 gagatacagc ccgtccccga agatggcgtc ctgctcgggc ccgaagagcc tggcggcgcg
     3121 aagcttgaac gattcgagct ccttatccga gaggcgcggt ttatcggcta gctggcgcgg
     3181 ccgttcgaga ggcgtcgcgc tgttgttctc ccagacgccc tgaagatccg gccggccgtc
     3241 ggccgtgcgc ggaacgaccc acgcgcttcc ccgaccttcc ggtctcgcag gctgctgcga
     3301 gttgcccggc gcataggtca gcgacacgag gacgagcgcg agcgcgacgc ggacgacacg
     3361 tgaactacgc accatacagt cctccggtca acatcatccc agctctcctt ccaggctgga
     3421 caccagcttt tccagttccg tcgccacggc tcgaaccgtg tcgggctggc ggagatcgac
     3481 gccggcgcgc gcgagcagat tcatcggata gtcgctgccc ccggcgcgca gcagcgtcaa
     3541 gtaccgttcg acagcgccgg cacgcgtgga gctgtcccca tcgcgtagat cctgcatcag
     3601 gcgcgcggtc gaggcaaagc aggtggcgta ctggtacacg tagtagggcg tgctgaagaa
     3661 gtgcggaatg cgcgcccacg tgatcgacga caacggctcc tcgtcgatga catctccgta
     3721 gtactcgcga agcagcgtgg cgtagatgcc gctcagcact tccgccgtga tcggccgatc
     3781 ctgctcgacg agccggtgcg cctggagctc gaagtccgcg aacatcacct gcgtgtagaa
     3841 cgtgctggcg atactgtcga tcgcgtgctg cagcagcacc gcccgctgct cgccgctgtc
     3901 ggcgcgctcg agcatgtagt ccaggaagag cgcttcgctc agcgtcgagg ggacctcggc
     3961 cacgaagatc gtataccccg cgtagacgaa cggctgggcc tgatgcgcca ggagcgtgtg
     4021 catcgagtgg cccatctcgt gagcgagagt aaacaccgcg tcgagtgtct cgttgtagtt
     4081 gagcagcatg taggggtgcg cgccgtacac gggcgcagag taggcgccgc tgcgcttgcc
     4141 catgttttcg aacacgtcga tccaccggcc ggcaaaggcg tcgcgcacgc cctgttgata
     4201 cccggctccg agcggcgcca ccgagtccac gatccacgat gcgacctgat cgtagccgta
     4261 gcgccgatcg tgctcgacga gtggaatgaa cacgtcggcc aggtggtagc ggtcgagccc
     4321 aagcacccgg cggcgcagcc tgtggtaccg ctggagcggg ccgatgccct ccttcgtgac
     4381 cgcgatgagc gtctcgacga cggaaggggg aatgtcgttg ccgtgcagcg cggcgtcgag
     4441 cgtcgtggca tagccgcggg ccctggcgtg gaaccagtcg cgctggagca cgccgttata
     4501 gagcgcggcg taggtattgc ggttgcccgc gtagacgccg tggaaggcac gataggccgc
     4561 gactcgatcc gcctggtttc gattggtctc gagcagcgcg cggtactgcc cgtagctcag
     4621 cgtgacgcga tcgccggcgg caagcgtgac gaccggcggc gtcatgtcgg ccgtggtcag
     4681 cgccgagtag ctgtcgtgcg gc
//
LOCUS       DBNGSH010000833         5661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2306426, whole genome shotgun sequence.
ACCESSION   DBNGSH010000833 DBNGSH010000000
VERSION     DBNGSH010000833.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2306426"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="AC16CY_19155"
     CDS             complement(<1..605)
                     /locus_tag="AC16CY_19155"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3355977.1"
                     /translation="MDQPLIKLESVRKVFYTDEVETHALDNINIEINRGEYVSIAGPS
                     GCGKSTLLSILGLLDTPTDGSYVLNSRPATALSLSERARVRNREVGFIFQSFNLIGDL
                     TVYENVELPLTYRGMRGAERKQRVTAALERVGMAHRAKHLPSQLSGGQQQRVAVARAV
                     CGEPSILLADEPTGNLDSKNGEAVMELLSELHKSGATICMVT"
     gene            complement(634..1632)
                     /locus_tag="AC16CY_19160"
     CDS             complement(634..1632)
                     /locus_tag="AC16CY_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3355978.1"
                     /translation="MRILLRPGAQVGPETVILELSNPQVEQDVVSARLTLLSAEASLD
                     NLRVRLENDLLTQRSQAAALKSQFEVARMDAEAKEALAKEKLVSEIDRRKAQNLAELL
                     KSQVALEEQRLATARESINAQLRVQQATVDQARTMADLQSNRLKSLRLTAGFSGVLQQ
                     VPVEVGQQVAQGQNLARVADPSRLKAELKIAETQAKDIEIGQSAEIDTRSGIIPGRVS
                     RKDPAAANGTVTIDVSLTGELPRGAVPDLSVDGTVQLERLDNILHVGRPSLGQDDTTV
                     GLFKLIDASGTAIRVQVSLGKSSVNSIEVKSGLNEGDRVVLSDMSAWDAYNRVRLR"
     gene            complement(2039..3232)
                     /locus_tag="AC16CY_19165"
     CDS             complement(2039..3232)
                     /locus_tag="AC16CY_19165"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3355979.1"
                     /translation="MRLEIRRRMTEGSVPRVLVADDQVDVVVALRLLLRNAGLEVDPA
                     TSVQEVRQRLGSQEYDLVLMDLNYARDTTSGREGLDLLAEIHARDRLLPVIVMTGWGS
                     IETAVEAMRRGARTFVHKPWDNQALAETVRKEIEDGLALRRAESLASTEQADAQAIQR
                     SLLPAALPVLPGCDLAARWEPAKAFGGDCYDAVRLTDTHLAISIADVCGKGLPAALLM
                     SNLMASLRAFTAVGRAPRDVVTSTNRALCRNSDLRRFVTLFYAVYNTGTRLLTYTNAG
                     HNPPIVARRDGSIERLTTGGMVTGIFEDTGYQESGVTLEPGDRLVLFTDGITEARASS
                     GAEFEDEGLLDVVARHRSGNVQSLIGAIFDEVASFAGGRLQDDSTVLAIAIESPSRGR
                     TDASH"
     gene            3400..3756
                     /locus_tag="AC16CY_19170"
     CDS             3400..3756
                     /locus_tag="AC16CY_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3355980.1"
                     /translation="MARDVLVLVGNMDEVPYGALDLMVLKTLAGLGPLHGYGIARRIE
                     QVAEGALALNQGTIYPALLRLEQKGWIKSEWGTSENNRRARFYSITRTGLKQLSVEAD
                     LWSRTVAMMSRLLEES"
     gene            3830..>5661
                     /locus_tag="AC16CY_19175"
     CDS             3830..>5661
                     /locus_tag="AC16CY_19175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3355981.1"
                     /translation="MRVFRVGIGRVLTLFRRGRFDAELDDEIRAHLELLARSHERRGM
                     APGEARLAARRDFGGVEQMKEIYRDRRGLPWMDDARRDVQHVLRALRRAPVFTAAAVL
                     TLAVGLTAVTAIFAILNAFMFRPLPVSHPEQLVSIGTGPDSHVQIPHGISFPDLQDYR
                     ERQASFSDLLGYQVGVGALDIGNATERVTTYEVTDNYFSMLGVEPALGRLIQPGEGKA
                     RGDAPVLVLTHEFWLARFGGDPSVVGRGVRLNGRPLTIIGVTPQSFDRAHSLIRPSAY
                     VPLWMHDDLMNNPATESILESRASHQNWVLGRLNPGVTLSQARAALQVTTAALAREYP
                     ATNKDASLVVIPETHARPNPNIGPMFRVAGMAFAVLAVILLLITSANVTNLLMARAVT
                     REREVALRAALGAGRGRLVRQLLTESVVLAMLAGVATLPVVVLALRRLERETAASTAL
                     ATLRPDFSLDPSVLAAALMLTLVAGIVSGLAPALMATRSGLNVALRAGGRGAGKSRAR
                     FRSALVVAQVALSLMLLVSGGLFVRSLDRARQVELGFDPDNVLVATAVPGETGYDSEQ
                     RRAYYAQVRERILRLAGVERAAWTQWAPLATVSDSARVWLESRPP"
BASE COUNT          873 a         1888 c         1867 g         1033 t
ORIGIN      
        1 gtcaccatgc agatcgtcgc cccgctcttg tgcagctcgc tgagcagctc catcacggcc
       61 tcgccgttct tcgagtcgag gttgcccgtc ggctcgtcgg ccagcaggat cgacggctcg
      121 ccgcagacgg cccgggccac cgcgacgcgc tgctgctggc cgcccgacag ctgactcgga
      181 aggtgcttgg cgcggtgcgc catgcccacg cgttcgagcg ctgccgtgac gcgctgcttc
      241 cgctcggccc cgcgcatccc tcgataggtg agcggcagct cgacgttctc atacacggtg
      301 agatcgccaa tgaggttgaa gctctggaag atgaagccga cctcgcggtt gcgcacgcgg
      361 gcacgctccg agagcgaaag cgcagtggcc ggccggctgt tgagcacgta gctgccgtcg
      421 gtcggcgtgt cgagcagacc caggatcgac agcagcgtcg acttgccgca gcccgagggg
      481 ccggcgattg acacgtactc gccccggttg atctcgatgt tgatgttgtc gagcgcgtgc
      541 gtttcgactt cgtccgtgta aaacaccttc cggacgctct cgagcttgat cagcggctgg
      601 tccattcctc agctcctgtc tgatgtgcca cccttacctc agtctcaccc ggttgtacgc
      661 atcccacgcc gacatatccg aaagcaccac ccggtcgcct tcgttcaatc ccgatttgac
      721 ctctatcgag ttcaccgaac tcttgccgag ggacacctgc acgcggatcg cggtacccga
      781 cgcgtcgatc agcttgaaca ggcccaccgt ggtgtcgtcc tggccgagcg acggtcgccc
      841 cacgtggagg atgttgtcca gccgttcgag ctggacggtg ccgtcaacgc tcaagtcggg
      901 cacggccccc cgcggcagtt cgcccgtcaa tgagacgtca atcgtgaccg taccgttcgc
      961 tgccgccgga tcttttctgc tcacccgccc tggaataatc ccgctgcggg tatcaatttc
     1021 ggccgattgc ccgatttcga tgtccttggc ctgggtctca gcaatcttca gttcggcctt
     1081 gagccggctg ggatcggcga cccgggcgag gttctgcccc tgggcgactt gctggccgac
     1141 ttccaccggc acctgctgca aaacgcctga aaaccccgcc gtgagccgga gcgacttgag
     1201 ccggttgctc tgcagatcgg ccatcgtgcg cgcctggtca accgtcgcct gctgcacgcg
     1261 cagctgcgcg ttgatcgatt cccgcgccgt ggccagccgc tgctcttcga gcgcgacctg
     1321 gcttttcagc aattccgcga gattctgcgc cttccttcgg tcgatctcgg aaacgagctt
     1381 ctccttggcc agcgcctctt tggcctcggc gtccatgcga gccacctcga actgggactt
     1441 gagcgccgcg gcctgcgatc tctgggtgag gaggtcgttc tcgagtctca cgcgcaggtt
     1501 gtcgagcgat gcctctgccg aaagcagcgt cagacgcgcg ctgaccacgt cctgctcgac
     1561 ctgggggttg ctcaattcca ggatgaccgt ctcgggtccg acctgggcgc caggtctcaa
     1621 caggattcgc acgacgcggc cggacgtctc cgatggcagc caccgtgtgt cctcgggcac
     1681 tagtgtcccg agtccccggt gctggcggac gatcgacccg cgcttgaccg tgtccacgag
     1741 caccgtttct tgcgggaccg tcggagccgc cggttccatg cgggccaggg caatcgtgac
     1801 tccgatcacc acgagcacgc cgacgcccgc atagagggcg tgcctcagct tcttccgttt
     1861 gagaatcgcc gggtcgcgtg caatgtcagg cattgatcag ccggtcgctt gtagccgttg
     1921 gccggtagct ccccaatcct acggtcaaag cgtatgccac acggggccgg gagttaaata
     1981 ctttgttcgc cgccgcttga ggtgggggag aggcacgcca ttcccgcggc gctgttcgct
     2041 agtgggacgc gtctgttcgg ccacgggaag gggactcgat ggcgatggcc agcacggtgg
     2101 agtcatcctg aagccggccg ccggcaaacg aggcgacctc gtcgaagatg gctccgatca
     2161 gcgactgcac gttgccggaa cggtgacgcg cgacgacgtc gagtaatccc tcatcctcga
     2221 attcggcgcc ggacgaggcc ctcgcctcgg tgatgccgtc ggtaaagagc acgaggcgat
     2281 cgcccggttc cagcgtgacg ccgctctcct ggtagcccgt gtcctcgaat attccggtca
     2341 ccatccctcc cgtcgtgagc cgctcgatcg acccgtcgcg gcgggcgacg atgggcggat
     2401 tgtggcctgc attggtgtag gtcaggagcc gtgtgccggt gttgtagacc gcgtagaaca
     2461 gcgtgacgaa gcgacggaga tcgctgttgc ggcagagcgc ccgattggtg ctggtcacca
     2521 cgtcgcgtgg cgcacgtcct accgccgtga acgcccgcaa cgacgccatc aggttcgaca
     2581 tcagcagcgc cgccggcagc ccttttccgc acacatcggc aatcgagatc gccaggtgcg
     2641 tgtccgtgag gcgaacggcg tcgtagcagt ctccgccgaa cgctttcgct ggttcccacc
     2701 gtgcggcaag gtcacatccg gggagcacgg ggagcgcggc tgggagcagc gaccgctgga
     2761 tggcctgggc gtcggcctgt tcggtcgatg cgagcgactc ggcgcgtcgc agcgcgaggc
     2821 cgtcctcgat ctccttgcgc acggtttcgg ccagtgcctg gttgtcccat ggcttgtgca
     2881 cgaacgttct ggcgccgcgc cgcatggctt cgactgcggt ctcgatgctg ccccagccgg
     2941 tcatgacgat cacgggcagc aggcgatcgc gtgcgtgaat ctcggccagc aggtcgaggc
     3001 cttcgcggcc cgacgtcgtg tctcgggcgt agttgaggtc catcagcacc aggtcgtact
     3061 cctgactgcc gagacgctgc ctgacctcct ggaccgacgt cgcgggatcg acctcgagtc
     3121 ccgcgttcct gagcaacagg cgcagcgcca cgacgacgtc cacctggtcg tcggcaacga
     3181 gcacgcgcgg caccgagccc tccgtcatgc ggcggcggat ctcaagccgc atgccaagct
     3241 caatcggatg gtggatggtg gatggtggtt ggcggacgat ggccattgcc tgtctgcgca
     3301 cgtgcggtgg cgaacacacg ccgtcccaca ggcggacagg gcgagaaact cctgactgac
     3361 ccctgccccc tgccccctag ccccctgttg tttatctaca tggcacgcga cgtgctcgtg
     3421 ttggtgggca acatggacga agttccgtac ggcgccctgg atttgatggt gctcaagacg
     3481 ctggcggggc tcggaccgct gcacggctac ggcatcgcgc gacggatcga gcaggtcgca
     3541 gaaggtgcgc tcgccttgaa ccagggaacg atctatccgg ccctgctccg gctggagcag
     3601 aagggttgga tcaagagcga atggggcacg agcgaaaaca accgtcgcgc ccgcttctat
     3661 tcgattaccc ggacgggcct gaagcagctg tccgtggaag ccgatctgtg gtcccggaca
     3721 gtcgcgatga tgagccggct gctggaggaa tcgtagagca ggggcttcag cccccgcgga
     3781 cacgcgcagg ctgaagcctg cgctctacgc cgccgagaca ggagacccaa tgcgtgtttt
     3841 tcgcgtcggc atcggacgcg tgttgacgct ttttcgacga ggtcgcttcg atgccgagct
     3901 cgacgacgaa atccgcgcgc acctggagtt gctcgcgcgt agtcacgaac ggcgtggcat
     3961 ggccccgggc gaagcgcgcc tggccgcgcg gcgcgatttc ggaggagtcg agcagatgaa
     4021 ggagatctac cgcgatcggc gtggactgcc gtggatggac gacgcgcggc gtgacgtgca
     4081 gcatgtcctg cgcgccttgc gccgcgcacc ggtgtttacc gcagcggccg tgctgacgct
     4141 ggcggtcggc ctcacggcgg tcacggccat cttcgcaatc ctgaacgcct tcatgttccg
     4201 gccgttgcct gtgagtcatc ccgagcagct cgtgtcgatc ggcaccgggc ccgattctca
     4261 cgtgcagatt ccgcacggga tctcgttccc cgacctccag gactaccggg agcgccaggc
     4321 gagcttcagc gatctcctgg ggtaccaggt cggggtcggc gcgctcgaca tcggcaacgc
     4381 caccgagcgc gtgaccacct acgaggtcac ggacaactac ttctcgatgc tcggtgtcga
     4441 gcctgcgctc gggcgattga ttcaacccgg tgagggcaag gcccgaggag atgcacctgt
     4501 gctcgtgttg acgcacgagt tctggctcgc ccgcttcggc ggagatccgt cggtcgtcgg
     4561 tcggggagtc cgcctgaatg gacggccgct cacgatcatc ggggtgaccc cgcagtcctt
     4621 cgatcgggca cactcgctca tccgcccgtc ggcgtatgtg cccttgtgga tgcacgacga
     4681 cctgatgaac aacccggcaa cggagtcgat cctggagagc cgcgccagcc accagaactg
     4741 ggtactcgga cgtctgaatc ccggcgtgac gctgtcacag gcacgagccg cgttgcaggt
     4801 gacgacggcg gcacttgccc gggagtatcc agcgacgaac aaggacgctt cgctggtcgt
     4861 catccccgag acgcacgccc gtccgaaccc gaatatcggt ccgatgttcc gcgtggccgg
     4921 gatggccttt gcggtgctgg cggtgatcct gctgctcatc acgagcgcca acgtcaccaa
     4981 cctgctcatg gcccgggccg tgacgcgcga gcgcgaggtc gcgctgcgcg cggcgctggg
     5041 cgcgggccga gggcggctgg ttcgccagct cctcaccgag agcgtcgtgc tcgcgatgct
     5101 ggcgggcgtc gccactctcc ctgtcgtcgt gttggcactg cgccgcttgg agcgtgaaac
     5161 agccgcaagc acagcccttg cgacgctgcg gccggacttc agcctggacc catccgtgct
     5221 cgccgccgcg ctgatgctga cgctcgtcgc cggaatcgtg tcagggctgg caccggcgct
     5281 catggccaca cgctccggtc tcaacgtggc gctcagggcc ggcgggcgcg gcgcgggcaa
     5341 gtcccgcgcg cggttccgca gcgcgctcgt cgtggcgcag gtcgcgctct cgctcatgct
     5401 gctcgtcagc ggcggcctgt tcgtgcgcag tctcgatcgg gcgcggcagg tcgagcttgg
     5461 ctttgatccg gacaacgtgc tcgtggccac ggccgttccc ggtgagactg gctacgattc
     5521 agagcaacgg cgggcgtact acgcccaggt gcgagagcgc attctccgcc tggccggggt
     5581 ggaacgcgcc gcatggaccc agtgggcgcc gctggccacc gtctcggaca gcgctcgcgt
     5641 gtggctcgag agccgacctc c
//
LOCUS       DBNGSH010000834         4518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3664015, whole genome shotgun sequence.
ACCESSION   DBNGSH010000834 DBNGSH010000000
VERSION     DBNGSH010000834.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3664015"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC16CY_19180"
     CDS             complement(<1..244)
                     /locus_tag="AC16CY_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355982.1"
                     /translation="MSFAIIVDRVRGEFMEMPDLELTMPQAIRLWSLGAEDCRSVIDA
                     LVDAGFLGWTAKRTIVRRGGDPMGPRKSRLTAISVPA"
     gene            535..3171
                     /gene="glgP"
                     /locus_tag="AC16CY_19185"
     CDS             535..3171
                     /gene="glgP"
                     /locus_tag="AC16CY_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR3355983.1"
                     /translation="MTTQAAAWTPGDISQLASQSGNSSETLTNIVNLIKQRFASDVCS
                     VYLLEPDRANLVLAATVGLRPESVGQVRMRVTEGLAGLVAEQARPVVVQDASAHPRFK
                     YFRDIGEEAYRTFLGVPVMDRSVLQGVLVIQTKEPRGFADDDVRMLTAAGTQLAPLVS
                     EARTEGQFVAPLYRRLAALARNLWWSWDTESVALFRDLDPILWQWCDHNPIALLQQID
                     LHQLEARATQLALHGRINHAYRRMQEYLHSKHTWGARHASVLGARPVAYFSAEFGLHE
                     SMPIYSGGLGILAGDHLKSASDLGIPLVGVGLFYDQGYFRQRLDADLWQHEEYVDTDH
                     RVLPMEPAMAAGAPVAIDIETRTGRIGARVWRTSVGRNTLFLLDSNVEGNSPEDRELT
                     ARLYGGDERVRIRQELLLGIGGVKALAALGISPGVVHLNEGHSAFASLELVRRRMDIE
                     GIGFDEAMRRVASHVVFTTHTPVPAGHDRFAPELVEEHLGPLRDALGLSREALLGLGR
                     VRPDDAGEEFCMTVLALKSSHRANAVSSLHGHVSRAMWRDLFPGLREEHIPIGHVTNG
                     VHVHSWLAPQMRLVYERLLGADWITRGSDPALGEAIDRIDDGELWEAHLALKARLITD
                     VRRRLVRQAERQGETPAVIAQLRRVLSRDALTIGFARRFATYKRANLLLQDVAGLEAL
                     VNHPQKPVQLVFAGKAHPLDGPGKTVLQEVATLARDPRFAGKVVFVEDYDIDLARHLV
                     QGVDVWLNNPRRPLEACGTSGQKVVLNGALNLSVLDGWWAEAYDGVNGFAIGRGDTHS
                     STAVHDARDGDALVDVLSHEVVRLYYERDRDGLPREWISRVKHAIRTLGWRFSADRMV
                     MDYVLKAYIPSVGGTSSDIRGS"
     gene            3232..4257
                     /locus_tag="AC16CY_19190"
     CDS             3232..4257
                     /locus_tag="AC16CY_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355984.1"
                     /translation="MGRWVRFSLVVLAALTPVVTASGQSSGAGAKGSYAPPKTSWGDP
                     DLQGIWPGTDMVGVPFERPDQFGTRLYLTDAELDERQKQAARQEEVDVLDFDLEKPPP
                     EIVALGDVGGVTSPPPHWLERGVPSRQSSLIVQPANGRMPAMTPEGAARQKTAGGTYT
                     KQTGFASAADLGPYDRCISRGVVGSMMPVVYNNGNQIIQAPGYVAFHNEMIHETRIIP
                     LDGRAPLPAAMKSYMGSSRGRWEGNTLVVRTTNLNGKTGMQGNGMMLIPSDALVLEER
                     FIPVSADVLQYEVTIDDPKTWTAPWKVSFPLKRDQEYQIFEYACHEGNHAMRNILSGS
                     RADERAR"
     gene            4273..>4518
                     /locus_tag="AC16CY_19195"
     CDS             4273..>4518
                     /locus_tag="AC16CY_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR3355985.1"
                     /translation="MAFRFLADATVVLHVAFVVFAVAGGLLVLRWPRVAWGHLPAATW
                     GAWVEFAGWICPLTPMEIWLRLRGGQSVYTVSFVYQYV"
BASE COUNT          769 a         1460 c         1545 g          744 t
ORIGIN      
        1 tggcgggcac ggaaatggcg gtcagcctgg atttccgagg acccattgga tccccgcctc
       61 tccgcacgat cgtgcgcttc gccgtccagc caagaaagcc ggcatcgacc agcgcatcga
      121 tgacagagcg gcagtcctca gccccgagcg accacagccg gatggcctgc ggcatggtca
      181 actcgaggtc cggcatctcc atgaactccc ctcgaacgcg gtcgacgatg atggcgaaac
      241 tcatggcctt cgcgtcagca accttcgggc catgcggatg tcggacgacg ccgacgagga
      301 ccgtggcgtg attccggtgt ttcctgcgcg tgcctccggc aacgacctca ctggattcac
      361 gcaacagtgt aggaacaccg actccgcgat acggaaatgt ctgatcggat ccacgcgact
      421 cgacagatga ggccgaaaag cgccgacagt gggcgtgcgt gagggcagtg ttcttgcttc
      481 gtcaggcgct tcgattctcc gggagggcgt tgattggaag gtgagggcgg agcaatgacc
      541 acgcaggcgg cggcctggac gccaggagac atcagccagc tcgcgtccca gagcggcaac
      601 tcgagcgaaa cgctcaccaa catcgtcaac ctgatcaaac aacggttcgc cagtgacgtg
      661 tgctccgtgt acctcctgga gccggaccgc gcgaacctcg tgctggcggc gacggtcggc
      721 ctgcgccccg aaagcgtcgg gcaggtgcgg atgcgtgtca ccgaggggct ggccgggctg
      781 gtcgccgaac aggcgcggcc cgtcgtggta caggacgcca gcgcgcatcc gcgcttcaag
      841 tacttccgcg acatcggcga ggaagcgtac cggacgtttc tgggcgtgcc cgtcatggac
      901 cgcagcgtgc tccagggagt gctcgtcatc cagacgaagg agccacgcgg attcgctgac
      961 gatgacgtgc ggatgctgac ggccgccggc acgcagctgg cgccgctcgt gagcgaagcc
     1021 cgcaccgagg gtcagttcgt ggcacccctg taccgcaggc ttgccgcgct tgcgcgcaac
     1081 ctgtggtgga gctgggacac cgaaagcgtc gccctcttcc gcgatctcga tccgatcttg
     1141 tggcaatggt gcgatcacaa cccgatcgcg ctgctccagc agatcgacct gcatcagctc
     1201 gaagcgcgcg ccacgcagct cgccctgcac ggccgcatca accacgcata ccgtcggatg
     1261 caggagtacc tccactcgaa gcacacgtgg ggtgcgcggc acgccagcgt gcttggtgcc
     1321 cggccggtcg cgtacttttc cgccgagttc ggcctgcacg agtcgatgcc gatctactcc
     1381 ggcggtctcg gcatcctggc gggcgaccac ctcaagagcg cctcggatct gggcattccc
     1441 ctcgtcggcg tcggcctctt ttacgaccag gggtacttcc ggcagcgtct cgacgccgac
     1501 ctctggcagc acgaggagta tgtggacacg gatcaccggg tgctgcccat ggagccggcg
     1561 atggccgccg gcgcgccggt cgccatcgac atcgagacgc gaacgggccg gatcggcgcg
     1621 cgcgtgtggc ggacgtccgt cgggcgcaac acgctcttcc ttctcgactc caacgtcgaa
     1681 ggaaacagtc cggaagatcg agagctgacc gcgcgcctgt acggcggtga cgagcgcgtg
     1741 cgcatccgcc aggagctgct cctcggaatc gggggagtca aagcgctggc cgcgctgggc
     1801 atctcacccg gcgtggtgca cctcaacgag ggacacagcg cgtttgcctc cctcgagctg
     1861 gtcaggcgca ggatggacat cgagggaatc ggcttcgacg aggcgatgcg gcgcgtggcc
     1921 tcccatgtgg tctttacgac tcacacgccg gtgccggccg gccacgatcg cttcgccccg
     1981 gagctggtcg aagaacatct cggtccgctg cgcgacgcac tgggcctgtc ccgcgaggcg
     2041 ctgcttggac tcgggcgtgt gcggcccgac gacgcaggcg aagagttctg catgacggtc
     2101 ctcgcgctga agtcgagtca ccgggccaac gccgtgtcgt cgctgcacgg ccatgtgtcg
     2161 agggcgatgt ggagggatct cttcccgggc cttcgagagg agcacattcc gatcggccat
     2221 gtcacgaacg gagttcacgt gcactcgtgg ctggcgccac agatgcgcct cgtgtacgag
     2281 cgcctcctgg gcgcggactg gatcacgcga ggcagcgacc cggccctcgg ggaagccatc
     2341 gaccggatcg acgatgggga gctctgggaa gctcacctgg cgctgaaggc tcgcctgatc
     2401 accgacgtgc gtcgccggct cgtgcgccag gccgagcgtc agggtgagac gccggccgtc
     2461 atcgcgcagc tccgccgggt gctgagtcgc gatgcgctca cgattggctt tgcgcgccgg
     2521 tttgccacct acaagcgcgc taatctcctc ctgcaggatg tcgcagggct cgaagcgctg
     2581 gtcaaccacc cccagaagcc cgtacagctc gtgtttgccg ggaaggcgca cccgctcgac
     2641 ggtccgggaa agaccgtgct gcaggaagtc gcgacgcttg cgcgcgaccc gaggttcgcg
     2701 gggaaggtcg tgttcgtcga agactacgac atcgaccttg cccggcacct cgtccagggc
     2761 gtcgacgtgt ggctgaacaa cccgaggagg ccgctcgagg cgtgcggcac gagcggacag
     2821 aaagtggtgc tcaacggggc gctcaacctc tccgtgctcg acggctggtg ggccgaggcg
     2881 tacgacggtg tgaacggatt cgccatcggt cgaggcgaca cgcacagttc gaccgcggtg
     2941 cacgacgccc gcgacggcga tgcgctggtt gacgtgctca gccacgaggt ggtgcggctc
     3001 tactacgaac gcgaccggga cggcctgcct cgggagtgga tctcacgcgt caaacacgca
     3061 atccggacgc tcggctggcg cttcagtgcc gaccgcatgg tgatggacta cgtgctcaag
     3121 gcgtacattc cctccgtcgg tggcacgagc agcgacattc gcggttcctg acggcgggcc
     3181 ctgtccaaca aggcgatatc atgaggactg gtcgtctcgc gggaggggtg gatgggtcgt
     3241 tgggttcgat tctcgctggt tgtcctggcc gcgctgacgc ccgtcgtcac ggcgtccgga
     3301 cagtcgtctg gtgcaggcgc gaagggcagc tacgcgccgc cgaaaacatc gtggggcgat
     3361 ccggatcttc aaggcatctg gccgggcacc gacatggtcg gcgtgccatt cgagcggccg
     3421 gaccagttcg gcacccgcct gtacctgacc gacgccgagc tcgacgaacg gcagaagcag
     3481 gccgcgaggc aggaggaagt ggacgtcctc gatttcgatc tggagaagcc gccgccggag
     3541 atcgtcgcgc tcggcgacgt cggaggagtg acctctccgc cgccgcactg gctggagcgc
     3601 ggcgtcccgt cgcgccagtc atcgctgatc gtccagccgg ccaacggcag gatgcccgcg
     3661 atgacacccg agggcgcagc caggcagaaa accgcgggcg gcacctacac gaagcagacc
     3721 ggctttgcga gcgcagccga cctcgggccc tacgaccgtt gcatctcgcg cggcgttgtc
     3781 ggatcgatga tgccggtcgt ctacaacaac ggaaaccaga tcatccaggc gccggggtac
     3841 gtcgccttcc acaacgagat gattcacgag acacgcatca tcccgctcga cggccgcgcg
     3901 ccgctgccgg cggcgatgaa gtcctacatg gggtcgtcga gaggccgctg ggagggcaac
     3961 acgctcgtgg tgcggacgac gaacctgaac gggaagaccg gcatgcaggg caacggcatg
     4021 atgctgattc cgagcgacgc gctggtgctg gaggagcgct tcattccggt gtcggccgac
     4081 gtcctgcagt acgaggtgac gatcgacgac ccgaagacgt ggaccgcgcc ctggaaggtc
     4141 tcgttcccgc tgaagcggga tcaggagtac cagatcttcg agtacgcgtg ccacgagggg
     4201 aaccatgcca tgcggaacat cctctcgggt tcgcgtgcgg acgaacgggc gcgctgagcc
     4261 gcctgtcgcc tcatggcttt ccggttcctg gctgacgcga ccgtagttct tcatgtcgcg
     4321 ttcgtcgtgt tcgcggtggc gggtgggctg ctggttctgc ggtggcctcg cgttgcctgg
     4381 ggacatcttc cggccgcgac atggggtgcc tgggtcgaat tcgcgggatg gatatgtccc
     4441 ctcacgccga tggagatctg gctccgactg cgcggcgggc agagcgtcta tacggtcagc
     4501 ttcgtgtacc agtacgtc
//
LOCUS       DBNGSH010000835         4364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1966748, whole genome shotgun sequence.
ACCESSION   DBNGSH010000835 DBNGSH010000000
VERSION     DBNGSH010000835.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1966748"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1902
                     /locus_tag="AC16CY_19200"
     CDS             <1..1902
                     /locus_tag="AC16CY_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3355986.1"
                     /translation="FVPVGEFRRLLDSGAPAPARAAAFAALARINTLYMIAGAWSGHI
                     GTSFSSLEIMSWLFLNEIRDLRKGDDACDIFFSSKGHDAPALYSVLIGLGLLPEEKLH
                     QLRRLHGLPGHPHVETPYVQANTGSLGMGISKAKGMALANRIAGVSRRIFVMTGDGEL
                     QEGQIWESLGSAATRGLAEIVVIVDHNKIQSDTWVQSVSPLGDLEAKFRAFGWHVARC
                     DGHDVAALQGTLREIAGVSTLPKVVIADTVKGKGVSFMEGPAMKEGELYAYHSGAPAE
                     DRYTAGLKELLAAANKAFGELGLGRVATESRTRNARREPRKTENLVAAYEQALVRQGE
                     RHERLVALDADLVKDCGLVSFAKRFPDRFVECGIGEQDMVSMAGGMARRGVLPVVHSF
                     ACFLSARPNEQISNQCTEGTRVIYVGSLAGLLPGGPGHSHQSVRDISALRGVPNLSLV
                     EPCLEAEVQSLLDYAVNTAADSVYIRLVSVKWPVPFSYPHQQRVEPGRGWVIRDGADA
                     VVFGYGPWLLSNAFDAAEQVEQSTGASVRLVSLPWLNRVDPRWLREVIGGRRYILTLD
                     NHYLKGGQGEMLATAIAELALEPTPRVLRVGVTALPECGTNDEVLAHHRLDVASLVKS
                     MRYVVPQIA"
     gene            1899..2606
                     /locus_tag="AC16CY_19205"
     CDS             1899..2606
                     /locus_tag="AC16CY_19205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006527555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3355987.1"
                     /translation="MTVLFWDIDGTLLTTGKAGVPAWEDAVREVVGFDFQLSSFRVAG
                     LTDFQIAVRTFEMLGVNADEDMLRRMVRRYEELLPLALPKKQGRVLPNVREILDRLEG
                     RADVRSYLLTGNTRGGARAKLMHYDLFRYFPDGAFAEDASERSVIASRALALARREGP
                     VAGEGVFVVGDTPHDIECAAAIGARTVAVATGGYSLEELESHRPWRAFAELPPADEFL
                     RLIDDARASRPSETLRA"
     gene            2603..4318
                     /locus_tag="AC16CY_19210"
     CDS             2603..4318
                     /locus_tag="AC16CY_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006530536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HIR3355988.1"
                     /translation="MKRGIRRFPRVWRASRLARRWYYARAGEYPDWSALVAAEPELWQ
                     SARAAAQGGPRVLMATSIGSYSHAATLESALAAALTLRGAEVHALLCDGAMTACADCD
                     ASLYPDMTHFVRHGPSRDLCRDCSWPAERVYAQLGITVHKYSDWLTPEDRDEARRVAS
                     TIPAGDIRSFTCHGLVVGEHAYAGALRFFATGSLDAEPMADAVLRRYLESALRTAFAT
                     RRLLRTIGFSSAVFTHGIYVPWGIVGEVARQEGVHVSTWNVAYRKRRFIFSHDDTYHH
                     TLMNEPREHWEDLDLDAGRERDLMHYLSSRRDGLFDWIVFHRPSTQDPKELPALLGLD
                     PSRPVIGLLTNVSWDAQIHYPANAFENMLDWLVQTCRYFARRPELQLLVRVHPAEISG
                     FPPSRQPILGELRKAIPVLPPNIVVVPPESELSTYALMSLCNSVVIYGTKMGVELTSR
                     GVPVIVAGEAWIRNKGLTLDASSPGEYFRLLDRLPLPEESDAARLARARRYAYHFFFN
                     RMIPLPFVEPRAGTSLYRLKLNSLKHLLPGQSDGLDTICRGILEREPFVLRDTLPRSP
                     ELVSS"
BASE COUNT          653 a         1501 c         1487 g          723 t
ORIGIN      
        1 ttcgtgccgg tgggcgagtt ccgccgcctg ctcgactccg gcgcgccggc ccccgcccgt
       61 gcggctgcct tcgcggccct ggcgaggatc aacacgctct acatgatcgc gggcgcctgg
      121 tccggccaca tcggcaccag cttcagctcg ctcgagatca tgagctggct gttcctcaac
      181 gagattcgcg accttcgcaa gggcgacgat gcgtgcgaca tcttcttctc ctcgaagggc
      241 catgacgcgc ccgcgctcta cagcgtgctg atcgggctgg gcctgctgcc cgaggagaag
      301 ctgcaccagt tgaggcgact gcacgggctg cccggccacc cgcacgtcga gaccccctac
      361 gtgcaggcca acaccggctc gctggggatg gggatctcga aggcgaaggg catggcgctg
      421 gccaaccgga tcgccggagt ctcgcgccgg atcttcgtga tgacgggcga cggcgagctt
      481 caggagggac agatctggga atctctcggg tcggccgcca cgcgcggcct ggcggagatc
      541 gtggtgatcg tcgatcacaa caagatccag tccgacacgt gggtccagtc ggtcagcccc
      601 cttggcgatc tcgaagccaa gtttcgtgcg ttcggctggc acgtcgcccg ctgtgacggc
      661 cacgacgtgg ccgcgctgca gggcacactt cgcgagatcg ccggcgtctc gacgctcccc
      721 aaggtcgtca tcgccgacac cgtgaagggg aaaggcgtgt ccttcatgga aggcccggcg
      781 atgaaggagg gcgagctcta cgcgtatcac tccggcgccc ccgcggagga tcgatacacg
      841 gccggcttga aggaacttct cgccgcggcc aacaaggcgt tcggagagct cggcctcggc
      901 cgcgtcgcca ccgagtcgcg cacgcgaaac gccaggcgcg agccgcgcaa gacggagaac
      961 ctcgtggctg cctacgaaca ggcgctcgtg aggcaggggg agcgtcacga gcggctggtc
     1021 gcgctcgacg ccgatctcgt caaggactgc ggcctcgtct cgttcgcgaa gcgctttccc
     1081 gatcgcttcg tcgagtgcgg gatcggcgag caggacatgg tctcgatggc cggaggcatg
     1141 gcgcggcgcg gggtgctccc ggtcgtgcat tccttcgcct gcttcctgtc ggcgcggccg
     1201 aacgagcaga tttccaacca gtgcacggaa ggtacgaggg tcatctacgt cggatcgctg
     1261 gccgggctgc tgccgggcgg cccgggccac tcgcaccagt cggtgcgcga catctccgcg
     1321 ctgaggggcg taccaaacct gtcgctcgtc gagccgtgcc tcgaggccga ggtgcaatcg
     1381 ctgctcgact acgctgtcaa cacggcggcg gacagcgtgt acatcaggct cgtgtcggtg
     1441 aagtggcccg tgccgttcag ctacccgcac cagcagcgtg tcgagcccgg ccgcggctgg
     1501 gtgattcgcg acggggcgga cgccgtggtc ttcgggtacg gtccatggct gctgtcgaac
     1561 gccttcgacg ccgccgaaca ggtcgagcag agcacgggcg cgagcgttcg cctggtcagc
     1621 ctgccgtggc tcaaccgcgt cgatccccgg tggcttcgcg aagtgattgg cggccgccgc
     1681 tacatcctca cgctcgacaa ccactacctc aagggcggcc agggcgagat gctcgccacg
     1741 gcgatcgcgg agctggcgct cgaacccacg ccgcgcgtcc tgcgcgtcgg cgtgacggct
     1801 ctccccgagt gcggcaccaa cgacgaggtg ctcgcgcatc accggctgga cgtggccagc
     1861 ctcgtcaaga gcatgcggta cgtcgtgccg cagatcgcat gacggttctc ttctgggaca
     1921 tcgacggtac gctgctgacg acgggcaagg ccggtgtacc ggcgtgggaa gacgccgtgc
     1981 gggaagtcgt gggcttcgac ttccagctgt cgtccttccg cgtcgcaggc cttaccgact
     2041 tccagatcgc cgttcgcacg ttcgagatgc tcggcgtcaa cgccgacgag gacatgctgc
     2101 gccggatggt gcggcgatac gaggagctgc tgccgctcgc gctgccgaag aagcagggcc
     2161 gtgtgctccc gaacgtccgg gagatcctcg atcggctcga ggggcgcgcc gacgtgcgct
     2221 cgtacctgct gacgggcaac acccgcggcg gcgcgagagc caagttgatg cactacgacc
     2281 tgttcaggta cttccctgac ggcgcgttcg cagaggacgc gagcgagcgg tcggtcattg
     2341 cctcgcgggc gctggcgctg gcgcggcgcg agggtcccgt ggccggcgaa ggcgtcttcg
     2401 tcgtcggcga cacaccgcac gacatcgagt gcgccgccgc catcggcgcg cggaccgtgg
     2461 ccgtggccac cggagggtat tcgctcgagg agctcgagtc ccaccggccc tggcgcgcgt
     2521 ttgcggaact gccgccggcc gacgagtttc ttcggttgat cgacgacgcc cgcgcaagcc
     2581 ggccaagcga gacgcttcga gcatgaagcg aggcatccgc cgttttcccc gcgtctggcg
     2641 cgccagccgt ctagcgcgac ggtggtacta cgcgcgcgcg ggcgagtatc cggactggag
     2701 cgcgctggtc gccgccgagc ccgagctctg gcagtcggcg cgcgcggcgg cacagggcgg
     2761 gccgcgcgtg ctcatggcca cctcgatcgg ctcgtactcg catgcggcca cgctcgagag
     2821 cgcgctggcc gccgccctga cgctgcgggg ggccgaggtg catgccctgc tttgcgacgg
     2881 cgccatgacg gcgtgcgccg actgcgacgc ctcgctctat cccgacatga cgcacttcgt
     2941 ccgccacggc ccttcgcgcg atctctgcag ggactgctcg tggccggccg agcgcgtgta
     3001 cgcgcagctc gggatcaccg ttcacaagta cagcgactgg ctcacgcccg aggatcgcga
     3061 cgaagcgcgc cgcgtcgcct ccacgattcc cgccggcgac atccggtcgt tcacgtgcca
     3121 cggcctcgtc gtcggggagc atgcctacgc cggcgccttg aggtttttcg cgaccggctc
     3181 gctcgatgcc gagccgatgg cggacgccgt tctgcggcgc tacctcgagt cggcgctgcg
     3241 cacggccttc gcgacgcgcc ggctgcttcg cacgatcggc ttctcctcgg cggtcttcac
     3301 gcacggcatc tacgtgccct ggggcatcgt gggggaggtc gcgcgccagg aaggcgtcca
     3361 cgtctcgacc tggaacgtcg cctaccggaa gcgccggttc atcttcagcc acgacgacac
     3421 ctaccatcac acgttgatga acgagccgcg cgagcactgg gaggatctcg atctcgacgc
     3481 gggccgcgaa cgcgacctga tgcactacct gtcgagccgc cgcgacgggc tcttcgactg
     3541 gatcgtgttt caccggccct ccacgcagga tccgaaggag ctgcccgccc ttctgggtct
     3601 cgacccgtcg aggcccgtca tcgggctgct caccaacgtg tcgtgggacg cgcagattca
     3661 ctacccggcc aacgcgttcg agaacatgct cgactggctc gtgcagacct gccgctattt
     3721 cgcgcggcgc ccggagctgc agctgctggt gcgcgtgcac cccgccgaga tcagcggttt
     3781 tccgccgtcg cggcagccca ttctcggcga gctgcggaag gcgattcccg tgcttccgcc
     3841 gaacatcgtc gtcgtgccgc ccgagagcga gctgagcacc tacgcgctga tgtcgctgtg
     3901 caacagcgtc gtcatctacg gcaccaagat gggcgtggag ctgacgagcc gcggcgttcc
     3961 ggtgatcgtg gccggggagg cgtggatcag gaacaagggg ctgacgctcg acgcgagctc
     4021 ccccggcgag tacttccggc tgctggatcg gctcccgctg ccggaggaat cagatgccgc
     4081 ccgcctcgcg cgcgcccgcc ggtatgccta ccacttcttc ttcaaccgga tgattccgct
     4141 gccgtttgtc gaacccaggg cgggcacctc gctctaccgg ctgaagctga actcgctcaa
     4201 acatttactg ccaggacagt ccgacggact ggatacaatc tgccgcggga tcctcgaacg
     4261 ggagccgttc gtgctcaggg acacgttgcc tcgcagcccc gagctcgtga gctcgtgatc
     4321 gcgtgattga ggttctgagt ttcctcgttg ccacggccgc cgcg
//
LOCUS       DBNGSH010000836         2395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2136664, whole genome shotgun sequence.
ACCESSION   DBNGSH010000836 DBNGSH010000000
VERSION     DBNGSH010000836.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2136664"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1283
                     /locus_tag="AC16CY_19215"
     CDS             108..1283
                     /locus_tag="AC16CY_19215"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR3355989.1"
                     /translation="MPVIVSAARIPTGKFLGALKDHTAPQLGAIVVREAVRRAGIDPA
                     LVDECIMGNVVSAGLGQAPARQAALRGGLPDSVAAMTINKVCGSGLKAVMLAAHAIAA
                     GDAHVVVAGGMESMSNCPYLLPGARQGLRMGHGQVVDSMINDGLWCAFEEWHMGNAGE
                     VVAEEYGVDRRAQDEFALASHRKAAEAASSGRFRAEIVPVAIPQKKGDPVIFDRDESV
                     RPDTSIEALAALRPAFKANGTVTAGNAPPVSDGAAALVVMSEQRARELRVTPLARIVG
                     QATSGLPPKYLLMTPVEAIRRVLMKAGWSLGDVDLFEINEAFSAQLVAVMSQLGIGPE
                     KVNVNGGAVAMGHPIGASGARILTTLLYALEQRGLRRGVAALCLGGGNGVALAVERA"
     gene            1565..>2395
                     /gene="recA"
                     /locus_tag="AC16CY_19220"
     CDS             1565..>2395
                     /gene="recA"
                     /locus_tag="AC16CY_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR3355990.1"
                     /translation="MPLEDVKERSKAIDIAVGQIEKQFGKGSIMRLGQRNAIAPIEAI
                     STGAISIDYALGVGGLPRGRVTEIFGPESSGKTTLALQVIAEAQRGGGMAAFVDAEHA
                     LDAQYAQKLGVDLENLLVSQPDNGEQALEIVEVLIRSNSVDVVVVDSVAALVPKAEIE
                     GEMGEAQMGLQARLMSQALRKLTGVVSKSKTTLVFINQLREKIGVMFGNPETTTGGRA
                     LKFYASVRVDIRRIASIKDGDQVIGGRTRVKVVKNKVAAPFREAEFDIMYGEGISREG
                     D"
BASE COUNT          436 a          774 c          808 g          377 t
ORIGIN      
        1 aaggaacaac gatcaccgct ccactcatgc tgcctcccgc ggttcttccc ccgctgacga
       61 tacaacgaag gtgacgggac ggcagttaac agggctaaga tctccacgtg cccgtcatcg
      121 tctccgctgc tcgcatcccg actggcaagt tcctgggggc gctcaaggat cacaccgctc
      181 cgcagctcgg ggccatcgtg gtccgcgaag ccgtgcgtcg agccggcatc gacccggccc
      241 tcgttgacga gtgcatcatg ggcaacgtgg tctcggccgg cctcggccag gcgccggccc
      301 ggcaagccgc gctcagggga ggacttccgg acagcgtggc cgcgatgacg atcaacaaag
      361 tgtgcggctc gggcctcaag gccgtaatgc ttgcggcgca cgcgatcgcc gccggcgatg
      421 cgcatgtcgt ggtcgcaggg ggcatggagt cgatgagcaa ctgcccctat ctgctgcccg
      481 gcgcacgcca ggggctgcgg atgggccacg ggcaggtcgt cgactcgatg atcaacgacg
      541 gcctgtggtg tgcgttcgag gaatggcaca tgggaaacgc gggagaggtc gttgccgagg
      601 agtacggcgt cgaccggagg gcgcaggacg agtttgcgct cgcaagccac aggaaggccg
      661 cagaggccgc ctcctcgggc cgctttcgcg ccgagatcgt cccggtggcc atcccgcaga
      721 agaagggcga cccggtgatc ttcgaccggg atgaatcggt gcgccccgac acgagcatcg
      781 aagcgctggc ggccctgcgc cccgcgttca aggcgaacgg caccgtcacg gccggcaacg
      841 ccccacccgt gagcgacggt gcagccgctc tcgtcgtgat gtctgaacag cgtgcgcgtg
      901 aactgcgtgt cacccccctc gcacggatcg tcggtcaagc cacgagcgga ctgccgccga
      961 agtacctgct gatgacgccg gtcgaggcga tccggcgggt actgatgaaa gccggatggt
     1021 cgctgggcga cgtggatctc ttcgagatca acgaggcgtt ctcggcccag ctcgtcgccg
     1081 tgatgtcgca gctcgggatc ggccccgaaa aggtcaacgt caacggcggc gccgtcgcga
     1141 tgggtcaccc gattggcgcg agcggggctc gcatcctcac gaccctgctg tatgcgctcg
     1201 aacagcgagg cctgcgccga ggcgtcgcgg cgctctgcct gggcggcggc aacggtgtag
     1261 cgctcgcggt agaaagggcc tagcagaatc agcatcgcct cccctctttt cgggcgtgcg
     1321 attcctgcga cgcgcggcgt gagaggcgaa agtcaggctg ttccgtaagt cattgataag
     1381 gctggctgga ctccatgtct ggttcacata ttttcgcttg actttcgcgt ggcacgtccc
     1441 taggatcgac ccgggccgtg accttctctg tcacaccacc aagccaccat cgaggcaccc
     1501 cgccgcgcgg ctgtgcgcga cggggacccc gctccagcca cggccacaac gccaaggaga
     1561 ccgcatgccg ctcgaagacg tgaaggaacg cagcaaggcc atcgacatcg ccgtcggtca
     1621 gatcgaaaaa caattcggca agggctcgat catgcgcctc gggcagcgca acgcgattgc
     1681 cccaatcgag gccatctcga ccggagccat cagcatcgac tacgcgctcg gggttggcgg
     1741 gctcccccgc ggccgggtga cggagatctt cggcccggaa tcatcgggca agacaacgct
     1801 cgcgctgcag gtgatcgccg aggcgcagcg cggcggaggc atggcggcgt tcgtggacgc
     1861 cgaacacgcg ctcgacgcgc agtacgcgca gaagcttggc gtcgatctgg agaacctgct
     1921 ggtctcgcag ccggataacg gcgagcaggc tttggagatc gtcgaggtcc tcatccgctc
     1981 gaacagcgtt gacgtcgtcg tcgtggactc ggtcgccgcg ctcgtgccga aagccgaaat
     2041 cgaaggcgag atgggcgagg cccagatggg gctccaggcg cggctgatgt cgcaggcgct
     2101 gcgcaagctc acaggggtgg tctcgaagtc gaagaccacg ctcgtcttca tcaatcagct
     2161 ccgcgagaag atcggcgtca tgttcggtaa ccccgaaacc accaccggcg gccgcgcgct
     2221 caagttctac gcgtcggtcc gcgttgacat ccgccgcatc gctagcatca aggacggcga
     2281 tcaggtcatc ggcgggcgca cgcgcgtgaa agtggtgaag aacaaggtcg cggctccatt
     2341 ccgcgaagcg gagttcgaca tcatgtacgg cgaagggatt tcacgcgagg gcgac
//
LOCUS       DBNGSH010000837         1972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_98712, whole genome shotgun sequence.
ACCESSION   DBNGSH010000837 DBNGSH010000000
VERSION     DBNGSH010000837.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_98712"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="AC16CY_19225"
     CDS             complement(<1..769)
                     /locus_tag="AC16CY_19225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIR3355991.1"
                     /translation="MTAFDLDRYLARIAWTGPRHASFETLAGVLRAHMTAIPFENLDV
                     LLGRGVRLDIDSVCAKLVAERRGGYCFEHGTLFRAALEGLGFEPEAHTARVIMERPRA
                     EAALTHMVLTVRVGGTAFVLDPGFGGHAPIVPVPLEPGQQVADETDLHRMARHGRDWV
                     LEARVDGAMQPLWTSTLEPAEPVDFVMANHFVSTFPESPFVRGLMLRAIAPRGRVSMF
                     NRDLTIRHPGGVEKSVIADRDGLHALLAEHFGFDLPDV"
     gene            complement(769..1806)
                     /locus_tag="AC16CY_19230"
     CDS             complement(769..1806)
                     /locus_tag="AC16CY_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3355992.1"
                     /translation="MCGCTVLILLTAGSLSAQTRAANASTGAAVSTWTPEKTPWGDPD
                     LQAIWSGDSAYGIPMQRPPELGNKAELTDQEFAAKVKQDEAARRRAENAVSSFRNDSA
                     WLERSFRQTSLVVDPPNGRVPALVAGAEARRTPQGTYGNGPLDSAADFTLYDRCVTLG
                     VVGSMTPKIYGNGYRIVQAPGYVVIMSEMIHEARVIPLDGRPHIGQGVRAYLGDSRGH
                     WEGNTLVIETTNFNGKAGTPGGNVLASTDLKIVERITRVEPELLRYEATISDPKTFAS
                     PYTISIPYTSPKGYQLLPYDCHEGNLAVLQGLGGERAEDRALEEDARRGVIRPRRAIQ
                     GELGVGGQPIR"
BASE COUNT          309 a          661 c          678 g          324 t
ORIGIN      
        1 tgacgtcggg cagatcgaag ccgaaatgct ccgcgagcag ggcgtgaagg ccgtcgcggt
       61 cggcaatcac gctcttctcg acgccgcccg ggtgccggat cgtcaggtcg cggttgaaca
      121 tggacacgcg tccgcgcggc gcgattgcgc gcagcatcaa tccgcggacg aacggggact
      181 ctggaaacgt ggacacgaag tggttcgcca tcacgaaatc gaccggctcg gccggctcga
      241 gcgtggacgt ccagagcggc tgcatcgctc cgtccacacg cgcttcgagc acccagtccc
      301 ggccgtgcct cgccatgcga tggaggtcgg tttcgtcggc cacctgttgt cccggctcga
      361 gaggtacggg cacaatcggg gcgtgtccgc cgaagccggg atcgagcacg aaggccgtgc
      421 cgccgacccg gaccgtgagg accatgtggg tcaacgccgc ctcggcgcgc ggccgctcca
      481 tgatgacgcg cgcggtatgt gcctcgggct cgaagccgag accttcgagc gccgcacgga
      541 acagcgtccc gtgctcgaag cagtagccgc cccggcgctc ggcgacgagc ttggcgcaga
      601 cgctgtcgat gtcgagacgc acgccgcggc caagcaggac atcaaggttc tcgaacggga
      661 tcgccgtcat gtgggcgcgc agcacgcccg caagcgtctc gaacgacgcg tgccggggac
      721 cggtccaagc gatgcgcgcg agataccgat cgagatcgaa ggccgtcact atcgaatcgg
      781 ctgtcccccg acgcccagct cgccctgaat cgcccgccgc gggcgaatga cgccacgcct
      841 agcgtcttcc tcgagcgccc tgtcttcggc gcgctcgccg ccgagcccct gcagcaccgc
      901 caggttgcct tcatggcagt cgtacggcag cagctgataa cccttgggcg aggtgtacgg
      961 gatggagatg gtatacgggc tcgcgaacgt cttggggtcg ctgatggtgg cttcgtagcg
     1021 aagcagctcc ggctcgacgc gcgtgatgcg ctcgacgatc ttcagatcgg tgctggccag
     1081 gacgttcccg ccgggcgtgc ccgccttgcc gttgaagttc gtcgtctcga tcaccagcgt
     1141 gttgccctcc cagtggccgc gcgagtcgcc aagatacgcg cgcacgccct gcccgatgtg
     1201 cgggcggccg tcgagcggaa tgacccgcgc ctcgtggatc atctccgaca tgatgacgac
     1261 gtagccgggc gcctgcacga tgcgatagcc gttgccgtag atcttcggcg tcatcgagcc
     1321 aacgacgccg agcgtgacgc agcggtcgta gagggtgaag tcggcggcgc tgtcgagcgg
     1381 gccgttgccg taggtgcctt gcggcgtgcg ccgcgcctcg gcgcctgcca cgagcgccgg
     1441 cacgcggccg ttgggcggat cgacgacgag agaggtttgc cggaacgacc gctcgagcca
     1501 ggcgctgtcg ttgcggaacg agctgacggc gttctccgcc ctccggcggg ccgcttcatc
     1561 ctgcttcact ttcgcggcga actcctggtc ggtcagctcc gccttgttcc ccagctccgg
     1621 cgggcgctgc atcggaatgc cgtacgccga gtcgccggac cagatcgcct gcagatccgg
     1681 gtcgccccac ggcgtcttct cgggggtcca cgtgctgacc gccgcgccgg tgctggcgtt
     1741 ggcggcacgg gtctgtgcgg acagcgaacc ggccgtcagc aggatgagaa cggtgcaacc
     1801 gcacattaca gagaatcgga tcctcataac ggctccgttc cgagaaaggt gtgaaggggt
     1861 ctgacccctg cgcattatcg ctccaatccg gcgaggcggt agcagcctcc cgtataatcg
     1921 cgccggcggt cgagctttcg ttggccttga cgatcggcag tcgtgtcggc gt
//
LOCUS       DBNGSH010000838         6740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3833896, whole genome shotgun sequence.
ACCESSION   DBNGSH010000838 DBNGSH010000000
VERSION     DBNGSH010000838.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3833896"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="AC16CY_19235"
     CDS             <1..194
                     /locus_tag="AC16CY_19235"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR3355993.1"
                     /translation="QIRRLMTRDALDEREAAARVAAQLPAEEKARRADYVIRTDGTYE
                     DTNRQVRAVFDQLVARRSG"
     gene            complement(236..1174)
                     /locus_tag="AC16CY_19240"
     CDS             complement(236..1174)
                     /locus_tag="AC16CY_19240"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIR3355994.1"
                     /translation="MKARTLDGQALALRMQEEIRPQVAAFVKAHGRPPGLGIVLVGAD
                     PASQVYVRNKVKAGGEAGFRVDLERMPESASLDDVLALVERLNRSAVHDGILVQSPLP
                     KGLGAGAQQRVFDAISADKDVDGFGAESVGLLVQNRPRLVACTPAGVIELLQREQIPI
                     KGRHAVVIGRSDIVGKPMALLLLHQDATVTICHSRTADLPSICRQADILVAAVGKAGI
                     VTRAFVKPGAAVIDVGMNRVTDRAVASQLFPAGHPKLASFDSKGSVLVGDVHPEVAEV
                     AGALTPVPGGVGPLTITMLMMNTLRAAQARTSSART"
     gene            complement(1171..2223)
                     /locus_tag="AC16CY_19245"
     CDS             complement(1171..2223)
                     /locus_tag="AC16CY_19245"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3355995.1"
                     /translation="MSGPHPQQVIRDFPTEVRDTWARGPRLAVLLILAILAVGTTGYV
                     LIEGWSAWDAFYMTVTTIMTVGYREVHPLSRQGQVFTVALIICGVGTVFYTATTLATI
                     LVEGGLHRGFERRRIARMLERVSDHFILCGHGRIGSIIAEELHAQGVPFVVIERSHER
                     TEQVKARGWIGIEDDASREEVLERAGIHRARGLIAAVGTDAENVFTVLTARVMRPDLY
                     IVARVESDDAEHKLRRAGADRVISPYQIGGAHMAQTALRPAVVDFVQLATRSGHLDLS
                     MEQVHIVDRSPLVGKSIVDAGIRQKFGVIIVAIKRIDGTMEFNPPPEAVIRAGDELVV
                     LGRPESVKALEETIAA"
     gene            complement(2546..3994)
                     /locus_tag="AC16CY_19250"
                     /pseudo
     CDS             complement(2546..3994)
                     /locus_tag="AC16CY_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(3995..4639)
                     /gene="thiE"
                     /locus_tag="AC16CY_19255"
     CDS             complement(3995..4639)
                     /gene="thiE"
                     /locus_tag="AC16CY_19255"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3355996.1"
                     /translation="MITLPRLHAIVDVEAAARANWEPAALARAFLDGGARCLQLRAKG
                     IAADRFLDLCDAVVRDARRYHALVIVNDRVDLALVSGAGGVHVGQDDFPPADARGLLG
                     PAAVIGYSTHTAAQIEEAAIEPVSYIAVGPVFGTQTKDTGYVAVGLDLVKEAVRRSRG
                     RPVVAIGGITLATAPGVWAAGASAVAIISDLLHGGDPGQRVASYNRLAPAVPGG"
     gene            complement(4636..6006)
                     /gene="accC"
                     /locus_tag="AC16CY_19260"
     CDS             complement(4636..6006)
                     /gene="accC"
                     /locus_tag="AC16CY_19260"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3355997.1"
                     /translation="MFKKILIANRGEIALRIIHACREIGIKTVAVYSEADENSLHVRF
                     ADQDVCIGPPRSADSYLNVPAIISAAEITGADAIHPGYGFLSESAYFAEVCEACHIRF
                     IGPDPSVIKLLGDKARAKKAMRKAGVPMLPGSDGPVTGEEQALKVAKDVGYPVIVKAV
                     AGGGGRGMRVVRNANDLPQALRTAQREAEAAFGNGDVYIEKYLESPRHIEFQIIGDHH
                     GNVVHLGERECSIQRRHQKLIEEAPSPALSDKIRKKMGSVVVDAAKAVQYTNAGTFEF
                     LMDGDGRFYYMETNTRLQVEHPVTEMVTGIDIVKEQIRIASGERLSFKQSEVTFTGHA
                     IECRVNAEHPETFAPSPGVIHAFSVPGGPGVRIDSFAHAECTVPPYYDSMIAKIIAHG
                     RDRQEAIARMRRTLEMTVIEGIHTSVPLHLKILNEPDFQAGRLSTSFMERFMPRQKKT
                     DLADTA"
     gene            complement(6010..6486)
                     /gene="accB"
                     /locus_tag="AC16CY_19265"
     CDS             complement(6010..6486)
                     /gene="accB"
                     /locus_tag="AC16CY_19265"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR3355998.1"
                     /translation="MDLEQIKKILDLMRDNDLAEFELEQDGVKVRLRKHPGPAEWSAA
                     PQPAPEGNARSATPQAPPASATPSQTLTVASEDVDLAIIKSPIVGTFYRAAEPGAQPF
                     ADVGQMVKKGQVLCIIEAMKLMNEINADCEGEVVKVYVENGQAVHYGERLFAIRPA"
     gene            complement(6547..>6740)
                     /locus_tag="AC16CY_19270"
     CDS             complement(6547..>6740)
                     /locus_tag="AC16CY_19270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3355999.1"
                     /translation="EPRIGPPRADSPTVQLEPGMVFTIEPGAYVPGLGGVRIEDDVVV
                     TESGCDVLTSVPRELQALS"
BASE COUNT         1193 a         2302 c         2117 g         1128 t
ORIGIN      
        1 cgcagatccg gcgattgatg acacgtgacg cgctcgacga gcgggaggcg gccgcacgcg
       61 tggccgccca gctgcctgct gaagagaaag cccggcgtgc ggactatgtg atcaggactg
      121 acggcactta cgaggacacg aatcggcagg tccgggcggt attcgatcag cttgtggcac
      181 gacgatccgg ctgaggccgg acaactctat ccaacggtcc ggctgaagcc ggacactacg
      241 tacgcgccga ggacgtccgc gcttgcgcgg cgcgcagcgt gttcatcatc agcatcgtaa
      301 tcgtgagcgg tccgacgccg ccgggcacgg gcgtcaacgc gcctgccacc tcggcaacct
      361 ccgggtgaac atcgcccacg agcaccgagc ctttcgagtc gaacgacgcc agcttcgggt
      421 gcccggctgg aaacaactgc gatgcgacag cccgatcggt gactcggttc atgccgacgt
      481 cgatgactgc ggcacccggc ttgacgaagg cccgcgtcac gattccggcc ttcccgacgg
      541 cagcgaccag gatgtccgcc tgccggcaga tcgacggcag gtcggccgtc cgcgaatgac
      601 aaatcgtcac cgtggcgtcc tggtgcagga gcagcagcgc catcggcttg ccgacgatgt
      661 cgctgcggcc gatgaccacc gcatgacggc ccttgatcgg aatctgctcc cgctgaagca
      721 gctcgatgac gccggccggc gtgcatgcca ccaggcgcgg acggttctga acgagcagac
      781 cgacgctttc ggctccgaag ccgtccacgt ccttgtccgc ggaaatcgcg tcgaacacgc
      841 gctgctgagc ccccgccccc agtcccttcg gcagcggcga ctgcacgagg atcccgtcgt
      901 ggacggcgct tcgattcaac cgctccacga gagccaggac gtcgtcgagc gacgctgact
      961 cgggcatccg ctcgaggtca acccgaaacc ccgcctcgcc gccggccttg accttgttgc
     1021 ggacgtagac ttgcgaggcc ggatcggccc cgacgagcac gatgccaagc cccggtggcc
     1081 ggccgtgcgc cttcacgaac gcggccacct gaggcctgat ttcctcctgc atccgcaagg
     1141 ccagtgcctg gccgtcgagc gtgcgagcct tcatgccgca atcgtctcct cgagtgcctt
     1201 tacgctctcc gggcgaccca gcaccaccag ttcgtctccc gcccggatga cggcctcggg
     1261 gggcgggttg aattccatgg tgccgtcgat ccgttttatg gccacgataa tgacgccgaa
     1321 cttctgccgg atacccgcgt cgacgatgct cttcccgacc agcggcgatc ggtccacgat
     1381 gtgcacctgc tccatcgaca ggtccagatg gcccgagcgt gtggccagct gaacgaagtc
     1441 gacgacggcc ggccggaggg cggtctgcgc catgtgggcg ccgccgatct ggtagggcga
     1501 aatgacgcgg tccgcccctg cccgcctcaa cttgtgctcg gcgtcgtcgg actccaccct
     1561 ggcgacgata tagaggtccg ggcgcatgac acgggcagtc agcacggtga acacattctc
     1621 ggcgtcggta ccgactgcgg cgatcagtcc gcgcgcccgg tggatgcctg cccgctccag
     1681 cacctcctcg cggctcgcgt catcctcgat gccgatccag ccgcgtgcct tgacttgctc
     1741 cgtccgctcg tggcttcgct cgatcacgac gaacggcaca ccctgggcgt gcagctcctc
     1801 ggcgatgatg ctgccgatac ggccgtgtcc acacagaatg aagtgatcag aaacccgttc
     1861 cagcatacga gcgatgcggc gccgctcgaa cccacgatgc agcccgccct ccaccaggat
     1921 tgtggccagt gttgtcgccg tgtagaagac cgtcccgaca ccgcagatga tgagcgccac
     1981 ggtgaagact tgaccctgcc ggctcagcgg atgcacctcg cgatagccga ccgtcatgat
     2041 ggtggtcacc gtcatgtaga acgcgtccca cgcgctccag ccttcgatca acacgtaccc
     2101 ggtcgtcccc accgcgagga ttgcgagaat gagcaggacg gcaagccgtg gaccgcgcgc
     2161 ccacgtgtcc cggacctctg tcgggaaatc gcgaatcacc tgttgagggt gtggtccgga
     2221 catcgtctgt cggtgcggcc gacggcgcca aatggccagt cgactacttc ggaacgcggg
     2281 cccggcccgc ggctctgatg atcaacccgg cgaggagcac gaacagccct gccccgagca
     2341 cttgaaggcc aaacgaatcg gcgtcttcgg gcgtcacacc gatctctgcc caccttgccg
     2401 tgacctcggt cgtcaccccg aactcggtca tgtacgccag caaggtgatg aagaacccgt
     2461 tgaagagcgt gagcccgccg gtcatcagca cgaagttccc gagggattcg acgatcggcc
     2521 gcctcttgcg ctcggccggg tcggcaaggg ggctgtgcgt ccgcccgtag aaccagtaga
     2581 tgaagatccc gatgtcgagc cagacaagga accgcaccca cgtgacaacg ggcaggttga
     2641 gcatcagcca cagacaggac acgatgccga gcaccgggaa caccatgccg ccagggacgc
     2701 ggaacggcct gtgcgcttcc ggccgctgca cgcggaggat caggacggcc gcgcagacga
     2761 ccacgaaggc aaagagcgtt ccgatgctcg tcatctcgcc gacgtcctgg atctgcgtca
     2821 ctcccgcgat caaagcgacc accaggcaag tgatgatcgt cgtgatgtac ggcgttccaa
     2881 aacgaggatg gaccttgctg atgcccttcg gcagcaggcc gtcgcggctc atggcaaaga
     2941 agatgcgcgg ttgagacatc aacatgacga gcagcacgct cgtgatgccg gcggccgcgc
     3001 ccgccgacac gaggtaggcg gcccaatcct gaccgatgag cgccagcgca aaggccaccg
     3061 gcgcgttcag aaactggctg acggcggtac cgtctgccgt cggcagccca gggagggccg
     3121 cagccgagga gcgatactgc gtgacaggca cgatcccgga caggatcgcc gcgactgcca
     3181 gatacagggc agtgcagacc accagcgacg caatgatgcc gatcggcaga tcccgacgcg
     3241 gattcctggc ttcctcggcc gtcgtcgata cggcatcgaa tccaatgtag gcaaagaaca
     3301 cgacggcggc ggcggccatg acgcccgatg tcccgtaggg cgcaaacggc acccagttct
     3361 ccggacggac ccaggtcaca ccgacggcga tgaagaacag gacggcgaaa cccttgatcg
     3421 ccaccatcgc ggcgttgaat ctcgcgctct cccggacgcc gatcaccagc agaaccatga
     3481 tgaccagcac gatgagcatg gccggcacgt tgatgatggt ccctggtgta accgccgacg
     3541 gcgctccgct catccagagt ggaagcgtga tcccggcgcc cgagagcagc cgctgcatgt
     3601 agccgctcca gccaatggcc acagtcatgg agccgacggc gtactcgagg atcaaatccc
     3661 agccgatgag ccacgcgaag atctccccca gcgaggcgta cgcgtaggta taggcgctgc
     3721 ccgcgatcgg caccatcgac gcgaactccg cgtagcagag cgcggcgaac ccgcaggcca
     3781 gcccggcagc cagatacgcc aggacgatgg cggggccggc ctggttggcc gccgccgtgc
     3841 ccgtgaggac gaaaatcccg gtcccgatga tcgcgccaac gccaagcagc gtcaggtccc
     3901 acgcgccgag actgcgccga agcgccttgc cgccttgctc gacatcgctc accaactgct
     3961 cgattgactt ggtcttgagc aggttcgaat ccattcagcc tcccgggact gcgggcgcga
     4021 ggcgattata gctggcgacc cgctggcccg ggtcccctcc gtggagcaag tcactgatga
     4081 tggcaaccgc ggatgctccg gctgcccaca ctcccggcgc ggtggcgagc gtgatgccgc
     4141 cgatggccac gaccgggcgg cccctggatc tccgcacagc ttctttcacc aggtcgagcc
     4201 ccacggcgac gtagccggtg tcctttgtct gcgtcccgaa caccgggccc accgcgatat
     4261 agctcacggg ttcgatcgcg gcctcctcga tctgcgcagc cgtgtgtgtc gaatagccaa
     4321 tgacggccgc cggaccgagc agaccgcgcg catcagccgg gggaaagtcg tcctgtccga
     4381 catgaacgcc ccccgcgccc gagacaaggg ccaggtccac ccggtcgttg acgatcacga
     4441 gggcatggta gcgtcgggcg tcgcggacga ctgcgtcgca gaggtcgagg aacctgtctg
     4501 cggcgatacc cttggctctg agctgaaggc accgcgcgcc gccgtccaga aacgcgcggg
     4561 caagcgccgc cggctcccag ttggcgcgtg cggcggcctc gacatcgacg atggcgtgaa
     4621 gccggggaag cgtgatcagg ctgtatccgc gaggtcagtt tttttttgcc gcggcatgaa
     4681 gcgctccatg aacgaagtgc tgagccgccc ggcctggaaa tcgggttcgt tgagaatctt
     4741 gagatgcaac ggcaccgagg tgtgaatgcc ttcgatgacg gtcatttcga gcgtgcggcg
     4801 cattcgggcg atcgcctcct gccggtcccg cccgtgggcg atgatcttgg caatcatcga
     4861 gtcgtagtac ggcggcaccg tgcactcggc gtgcgcgaac gagtcgatgc gcaccccggg
     4921 gcccccgggc acgctgaagg cgtggatgac gcctggcgac ggggcgaacg tctccgggtg
     4981 ctccgcattg acgcggcatt cgatggcatg ccccgtgaac gtgacctcgc tctgcttgaa
     5041 cgacagccgc tcgcccgacg cgatccggat ctgctccttc acgatgtcga tgcccgtcac
     5101 catctcggta acgggatgtt cgacctgaag acgggtgttc gtctccatgt aatagaacct
     5161 gccgtcgccg tccatcagga actcgaaggt tcccgcgttc gtgtattgca ccgccttggc
     5221 ggcatcgacc accacgctgc ccatcttctt gcggatcttg tcgctcaggg ccggcgacgg
     5281 cgcttcctcg atcagcttct gatgccggcg ctgaatcgag cactcgcgtt cgcccaggtg
     5341 cacgacgttg ccatggtgat cgccgatgat ctgaaactcg atgtggcggg gactctcgag
     5401 atacttctcg atgtaaacat cgccgttgcc gaaggccgcc tccgcctccc gctgcgcggt
     5461 ccgcagggct tgtggcagat cgttggcgtt gcggacgacg cgcatgccgc ggccgccacc
     5521 tccggcgact gccttcacga tgaccggata gccgacgtcc ttcgcgacct tgagcgcctg
     5581 ctcctcgccg gtcaccgggc cgtcgctgcc cggcagcatc gggacgcccg ctttcctcat
     5641 ggccttcttg gcccgcgcct tgtcacccag cagcttgatc acgctcggat cgggtccgat
     5701 gaacctgatg tggcaggcct cgcacacctc ggcgaaatag gcgctctccg acaggaagcc
     5761 atagccggga tggatggcgt cggcgccggt gatctcggcc gcgctgatga tcgccggtac
     5821 gttcaggtag ctgtccgcgc tgcgcggcgg accgatgcag acatcctggt ccgcgaaacg
     5881 gacgtggagc gagttctcgt cggcttccga atacaccgcc accgtcttga ttccgatctc
     5941 cctgcacgcg tggatgatgc gcagggcgat ctcaccgcgg ttggcaatca gaatcttctt
     6001 gaacataggc tacgcaggcc ggatggcgaa caaccgctcg ccgtagtgca cggcctgtcc
     6061 gttctcgaca tacaccttga cgacctcgcc ctcgcagtcg gcgttgatct cgttcatcag
     6121 cttcatggct tcgatgatgc agagcacctg acccttcttc accatctggc cgacgtcggc
     6181 aaatggctgc gcgccgggtt ccgccgcccg gtagaacgtg ccgacaatgg gcgacttgat
     6241 gattgccaga tcgacgtctt cgctcgccac cgtcagcgtc tgcgacggcg tcgcgctcgc
     6301 cggtggtgcc tgcggcgtgg ccgatcgtgc atttccctcc ggcgccggct gcggcgccgc
     6361 gctccactcc gccggacccg ggtgttttct caaccggacc ttgacgccgt cctgctcgag
     6421 ctcgaactcg gccaggtcgt tgtcgcgcat caggtccaga atcttcttaa tctgctcgag
     6481 atccatgcat ccgttccgtg cgtgtgcagg ggtcagaccc cttcgcacca ctgcacagtc
     6541 tgcccgtcac gagagcgcct gcaactcgcg ggggaccgac gtcagcacgt cgcacccgct
     6601 ctcggtcacc accacgtcgt cttcgatccg tacgccgccc aatccgggga cgtacgcccc
     6661 cggctcaatg gtaaacacca ttccgggctc gagttgcaca gtgggtgaat ccgcgcgagg
     6721 cgggccgatg cggggctctt
//
LOCUS       DBNGSH010000839         7124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4514155, whole genome shotgun sequence.
ACCESSION   DBNGSH010000839 DBNGSH010000000
VERSION     DBNGSH010000839.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4514155"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="AC16CY_19275"
     CDS             complement(<1..746)
                     /locus_tag="AC16CY_19275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3356000.1"
                     /translation="MRILVDYRPALSARTGVGEYIHSLLRAYTSAFDNDVIAFTSSWK
                     DRPSPALGAEVGARVVDRRVPVSLLNFLWHRVEWPPIESFAGDLDVVHASHPLLIPAR
                     RAAQVVTIHDLFFLDHPEQTRAEIRRDYARLTTRHAQRADAVITSSAHTKSLVGQRLK
                     VASDRIYVCPPGAPSWQSLGREPRVPADGYILFLGTLEPRKNLGVLLDAYVRLLDTAR
                     RVPRLLIAGGATPAAAPWLDRIARDPLKRVV"
     gene            793..2091
                     /locus_tag="AC16CY_19280"
     CDS             793..2091
                     /locus_tag="AC16CY_19280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR3356001.1"
                     /translation="MRIAIVGTGYVGLVAGACFAESGNDVACVDSDPAKVRSLQRGKV
                     PFYEPGLDELVRRNRSEGRLTFTTTLPKAVRNAAVIFVAVGTPQGEDGSADLSHVLEV
                     ARDVARSMNGYAVVVNKSTVPVGTSERVREIIRRETTHPFSVVSNPEFLKQGAAIDDF
                     LRPDRVIIGAEDPRAQAMMLELYEPLTRTGAPIMMMDCASAEMTKYAANAMLATRISF
                     MNEVANVCELVGADVDHVRKAIGSDKRIGPSFLFPGAGYGGSCFPKDVRAMRHFAADK
                     GYECRILAAVESVNAQQKTKLFAMLKKHYGALEGKTAGVWGLSFKPRTDDMREAPSIP
                     LIEAMLSAGMTVQAFDPEAIPAARRVFGSRITYAGSSYDAVKGADALVIVTEWNEFRL
                     PDFSRIRSLMRSPVVFDGRNLFAPALMKQNGFTYYSIGRS"
     gene            2078..3082
                     /gene="galE"
                     /locus_tag="AC16CY_19285"
     CDS             2078..3082
                     /gene="galE"
                     /locus_tag="AC16CY_19285"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR3356002.1"
                     /translation="MAAVEPTTVLVTGGAGYIGSHAAKALRAAGHRVVIYDNLSAGHR
                     EAALGAPLVEGDTGDVDCVRRAIRESGATAVMHFAAWLSVPDSVRDPAGFYRNNVAGT
                     VGTLEAMAAEKCRQFVFSSTCAVYGEPVDIPIRETHPTSPVNAYGQTKLAVEHALPHF
                     ERAYGIRSVRLRYFNAAGADPDGELGEDHSPEIHLIPRAIDAATGGPPLEIFGEDYPT
                     PDGTCLRDYVHVADLADVHLRALEWLQRDGASGAYNVGTERPSSVKDVIAAVERATGR
                     PVARRSAARRAGDPSVLYASAEPARRDLGWAPRRPDLDTIVADAWRWHSTHPLGFGSR
                     "
     gene            3108..4853
                     /gene="msbA"
                     /locus_tag="AC16CY_19290"
     CDS             3108..4853
                     /gene="msbA"
                     /locus_tag="AC16CY_19290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid carrier activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HIR3356003.1"
                     /translation="MNAIYRLAAYARPYRTRLAGALLAMAVYGAASGALTLLIKPIFD
                     KVLLKSEDLAWISIAVLAVYLLKGLGSYLSSYLMADVGQRVVRDVRNDLFRHMLGQSA
                     AFFALQTTGRLMARIMNDVGQLQRVVSDTVGDLTRETLTLVAFTGVLVYYDPRLALVC
                     LTGAPVVVYPLAQFGRRVRRTSRRSQEALEQMSHVTAEALTGHRIVQAFGAEPHEATK
                     FMQSSSRFYRTSMKVTSVLSLLPPLMELVGGVALVLALWYGSREIAAGRLTTGEFAAC
                     IAALFMMYAPARKLSRVNADLQQAASAAERVFEILETHSEVRDRPGAVPMRPFTKDIE
                     LRDVHFTYNGGEAFTLNGVSFTVHAGQTLAIVGRSGAGKTTLVNLVPRFYDPTGGAIL
                     IDGTDTREVAVSSLRSQIGIVTQETVLFDDTIATNIAYGRPSATRAEIEAAARSAHAH
                     EFIATLDAGYDTMIGERGQRLSGGQRQRIAIARAILRDSPILVLDEATSSLDAESESL
                     VQQALSNLMTNRTSFVIAHRLSTVRRADVIVVLDEGRIVESGTHEDLIAKGGPYAKLY
                     ELQLQEEDRPGVNAD"
     gene            5014..5907
                     /locus_tag="AC16CY_19295"
     CDS             5014..5907
                     /locus_tag="AC16CY_19295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR3356004.1"
                     /translation="MIKSMTGFASLTHEDERGTIAVTVRAVNHRFLDLQLRVPPLVSS
                     LESRLRALLQKRLARGRIELGMSVQLRDAGAPAVELNLDLAKALSAAVDRARELGLVA
                     GALTPGDLLRLPQVIAIREHAAEPDEALDARVAASVETAVDEALADLDAMRVREGAHL
                     AADLESRRERLGGLIEQLCVAAAEGQQALEARLRDRVDELRLELPVDQAMIAQEIVRL
                     AARSDISEEVTRFRAHLAHWRALSESDEPCGRKLDFLLQEMNREVNTIGSKADGIGVS
                     EIVIDAKAELERMREQVQNVE"
     gene            5897..6541
                     /gene="gmk"
                     /locus_tag="AC16CY_19300"
     CDS             5897..6541
                     /gene="gmk"
                     /locus_tag="AC16CY_19300"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR3356005.1"
                     /translation="MSNSLPGAGRLWRGQLFIVSAPSGTGKTTLVERLVQRVAGLRMS
                     RSYTSRPARGGEQDGVDYNFITRGRFEDMVREGAFLEWADVFGNYYGTSASDTDAMLA
                     RGEDVVLVIDVQGARQVRARGIQTIGVFVLPPSADVLEQRLRGRSKDSEEQIRRRLEV
                     AGAEVDEYAMYDYVVINDELDAAVERLRAIVGAERVRTRRMRAEIQAIIETFHS"
     gene            6538..6732
                     /gene="rpoZ"
                     /locus_tag="AC16CY_19305"
     CDS             6538..6732
                     /gene="rpoZ"
                     /locus_tag="AC16CY_19305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3356006.1"
                     /translation="MKSTPKENRFEFVVMASARARQLLKGAVPREAGDKKVTIAQREI
                     LRRKVEKIEERIVEKLEERP"
     gene            6745..>7124
                     /locus_tag="AC16CY_19310"
     CDS             6745..>7124
                     /locus_tag="AC16CY_19310"
                     /inference="COORDINATES: protein motif:HMM:NF014494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR3356007.1"
                     /translation="MSLIALGVTGGIGAYKAVEVCRGLQQRGHDVVAVMTRSATRFVG
                     PMTFEAITRHAVITDQWAPGLNAEIEHIKLTEQASLLLVVPATANLIGKLANGIADDF
                     LTSLYLATRAPVLVAPAMNTNMLG"
BASE COUNT         1193 a         2318 c         2507 g         1106 t
ORIGIN      
        1 acgacgcgct tgagcgggtc tcgcgcgatg cgatcgagcc acggggccgc ggccggcgtg
       61 gcgcctccgg caatcagcag gcgaggcacg cgccgcgccg tgtccagcag ccgcacgtag
      121 gcgtcgagga gcacgccgag attcttccgc ggctccagcg tcccgaggaa caggatgtac
      181 ccgtcggccg gcacgcgggg ctcgcgcccc agcgattgcc aggacggagc accgggtgga
      241 cagacgtaga tccgatcgga ggccaccttc aggcgttggc ccacgagcga tttcgtgtga
      301 gccgacgagg tgatcaccgc gtcggcacgt tgcgcgtgcc gggtcgtgag acgtgcgtag
      361 tcccgccgga tctccgcgcg cgtctgctcc ggatgatcga ggaagaacag atcgtggatg
      421 gtgacgacct gcgccgcgcg gcgtgcggga atcaggagcg ggtgcgaggc gtgcacgacg
      481 tcgagatcgc cggcgaacga ttcgatggga ggccactcga cgcggtgcca caggaagttg
      541 agcagcgaca cgggtacgcg ccggtctacg accctggctc ccacttccgc gccgagtgcg
      601 ggagacggcc gatccttcca cgagctcgtg aacgcgatga cgtcgttgtc gaacgccgac
      661 gtgtacgcgc gaagaaggct gtggatgtac tcgccgacac ccgtacgcgc gctgagagcc
      721 gggcgatagt cgacgagaat ccgcatgtgc cagccgtatg atagtaagta acggaggccg
      781 cgcagcagag ggatgcgaat agccattgtt ggtacggggt atgttggtct tgtcgctggt
      841 gcatgctttg ccgagtccgg gaacgacgtg gcgtgcgtgg acagcgaccc cgcgaaagtc
      901 cgctcgctgc agcgcggcaa ggttccgttc tacgaacccg gcctcgacga actggtccgg
      961 cgcaatcgga gcgaggggcg cctgacgttt acgaccacgc tgccaaaggc cgtccggaac
     1021 gccgccgtga tcttcgtcgc cgtgggaaca ccgcagggcg aggacggatc ggccgacctg
     1081 tcacacgtcc tcgaggtcgc ccgggacgtc gcgcgatcga tgaacggcta cgcggtcgtg
     1141 gtcaacaaga gcaccgtgcc cgtgggcaca tccgagcgcg tacgtgagat cattcgacgg
     1201 gagaccacgc acccgttcag cgtcgtcagc aatcccgagt tcctgaaaca gggggcggcc
     1261 atcgacgact tcctgcggcc ggaccgggtg atcatcggcg ccgaggatcc gagagcgcag
     1321 gccatgatgc tcgagctgta cgagccgctg acgcgcaccg gggcgccgat catgatgatg
     1381 gactgcgcca gcgccgagat gaccaagtat gcggccaacg cgatgctcgc gacgcgcatc
     1441 tcgttcatga acgaggtggc caacgtgtgc gagctggtgg gagccgacgt cgaccacgtg
     1501 cgcaaggcca tcggctcgga caaacggatc gggccgtcat tcctgtttcc gggtgccggc
     1561 tacgggggaa gctgctttcc gaaggacgtg cgtgcgatga ggcacttcgc cgccgacaag
     1621 ggctacgagt gccggatcct cgccgcagtc gagtccgtga acgcgcagca gaagaccaag
     1681 cttttcgcga tgttgaagaa gcactacggc gcgctcgagg gcaagaccgc cggggtgtgg
     1741 gggttgtcgt tcaagccgcg gaccgacgac atgcgcgagg ctccgtcgat tccactcatc
     1801 gaggccatgc tgtcggcggg gatgaccgtg caggcattcg accccgaggc catcccggcc
     1861 gccaggcgcg tgttcggttc ccggatcacc tacgcgggga gcagctacga tgctgtcaaa
     1921 ggcgccgatg cgctcgtcat cgtcacggag tggaacgaat tcagactgcc ggatttcagc
     1981 aggatccggt cgctcatgcg atcacccgtg gtgttcgacg gacgcaatct gttcgcgccg
     2041 gccctgatga agcagaacgg gttcacgtat tactccattg gccgcagttg agccgacgac
     2101 ggtcctggtc actggaggcg ccggctacat cggaagccac gccgccaagg ccctgcgtgc
     2161 cgcaggtcac cgcgtggtga tttacgacaa cctgtcggcg ggccaccgcg aggcggcgct
     2221 gggtgcgccg ctcgtcgagg gggatacggg ggacgtggac tgcgtgaggc gcgccatccg
     2281 cgagagcggc gcgacggcgg tgatgcactt cgcagcctgg ctgtcggtcc ccgattcagt
     2341 gcgcgatcca gccggcttct accgcaacaa cgtcgcgggg acggtgggaa cgctcgaggc
     2401 gatggccgcg gaaaagtgcc ggcagttcgt gttctcgtcc acctgcgccg tgtacggcga
     2461 gccggtggat atcccgatcc gtgagacgca tccgacgtcg ccggtcaatg cctacggtca
     2521 gacgaagctc gccgtcgagc acgccctgcc gcatttcgag cgcgcctacg gcatccggtc
     2581 cgtccggctg cggtacttca acgcggccgg cgccgatccg gacggcgagc tcggagagga
     2641 ccactcgccg gaaatccacc tcattccgag ggcgattgac gccgcaacag gggggccgcc
     2701 gctcgagatc ttcggcgagg actatccgac gccggacggc acctgtcttc gcgactacgt
     2761 ccacgtggcg gacctggccg acgtccatct gcgcgcgctc gagtggctgc agcgtgacgg
     2821 cgcgtccggc gcctacaacg tcgggaccga gcgtccctcg tcggtgaagg acgtgatcgc
     2881 ggccgttgaa cgtgcgacag ggcgtccggt cgcgcggcgt tcggcggcca ggcgcgcggg
     2941 ggatccctcg gtcctctacg cgtccgcgga gccggctcgg cgcgacctgg gctgggcgcc
     3001 gaggcgcccg gacctggata cgattgtggc ggacgcgtgg cgctggcact ccacacatcc
     3061 gctcgggttc gggagccgct gaggagacga tcggagtgga cggcggcatg aacgcgatct
     3121 accgcctggc cgcctacgcg cggccgtatc ggacgcggct tgccggcgcg ctcctcgcga
     3181 tggcggtcta cggcgcggcg tccggagcgc tgacactcct catcaagccg atcttcgaca
     3241 aggtcctgct gaagagcgag gatctcgcgt ggatctcgat cgccgtcctc gcggtgtacc
     3301 tgctcaaggg cctcggttcc tacctgtcgt cctacctgat ggccgacgtc ggccagcggg
     3361 tcgtgcgcga cgtccggaac gacctgttcc gtcacatgct cggccagtcc gccgcgttct
     3421 tcgcgctgca gacaacgggc cggctgatgg cccgaatcat gaacgacgtc ggccagctcc
     3481 agcgtgtcgt gtccgacacg gtcggcgacc tgacgcggga gacgctgacg ctggtcgcct
     3541 ttaccggggt cctcgtgtac tacgacccca ggcttgcgct cgtctgcctg accggcgcgc
     3601 cggtcgtcgt gtacccgctc gcgcagttcg gccggcgcgt ccggcgcacc agccgccgca
     3661 gccaggaagc gctcgagcag atgtcgcacg tcacggccga agcgctcacc gggcaccgga
     3721 tcgtgcaggc gttcggggcg gagccgcacg aggcgaccaa gttcatgcag tcctcgtccc
     3781 gcttctaccg caccagcatg aaggtcacca gcgtgctgtc gctcctgccg ccgctgatgg
     3841 agctggtcgg cggcgtggcg ctcgtgctcg cgctctggta cgggagccgg gagattgccg
     3901 ccggcaggct gacgacgggc gaattcgccg cctgcatcgc cgcgctcttc atgatgtacg
     3961 cgccggcgag gaaactgagc cgcgtgaacg ccgatctgca gcaggccgcg tccgccgccg
     4021 aacgcgtgtt cgagattctc gagacgcaca gcgaggtgcg agaccgtccc ggcgcggtgc
     4081 cgatgcgtcc gtttacgaaa gacatcgagc ttcgcgacgt gcacttcacc tacaacggcg
     4141 gggaagcgtt cacgctcaac ggcgtgtcgt tcacggtgca cgccggccag acgctcgcga
     4201 tcgtcgggcg gagcggcgcg ggcaagacga cgctggtcaa cctcgtcccg cggttctacg
     4261 acccgacggg cggcgccatc ctcatcgacg gcacggacac gcgcgaggtt gccgtgtcgt
     4321 cgctccggtc gcaaatcggc atcgtcacgc aggagaccgt gctcttcgac gacacgatcg
     4381 ccaccaatat cgcctacggc cggccctccg cgacccgcgc ggagatcgag gcggcggcgc
     4441 ggtctgccca tgcgcacgag ttcatcgcga cgctcgacgc gggctacgac acgatgatcg
     4501 gcgagcgcgg ccagcgcctc tcgggtggcc agcggcagcg catcgcgatc gcccgggcga
     4561 ttctgcgcga ctcgccaatt ctggtgctgg acgaggcgac ctcgtcgctc gacgccgaat
     4621 cagagtccct cgtgcagcag gcgctgtcga acctgatgac gaaccggacg tcgttcgtga
     4681 ttgcccatcg cctctcgacc gtccgtcggg ccgacgtcat cgtcgtgctc gacgaagggc
     4741 gaatcgtcga gtcggggacg cacgaggatc tgatcgcgaa gggcggcccc tatgcgaaac
     4801 tctacgaatt gcagctgcag gaggaagacc gtccaggggt gaacgcagac tgacgggacg
     4861 ctagtggtac gtccttcgcg gattactgcc ggccgcgccg ctcccacccc accgcgcaca
     4921 caccgcgcgg cggggacccc ggttgtcgcg gccggaaaat cgctgtcgaa gctcgttgtt
     4981 cgcggtactc ttccggttga tacggtccac tagatgatca agtccatgac cgggttcgcg
     5041 tcgctgactc atgaggacga gcggggcacc atcgccgtga ccgttcgtgc cgtgaatcac
     5101 cggttccttg acctccagct ccgcgtgccg ccgctcgtga gcagcctcga atcgcggctg
     5161 agagcgctgc tgcagaagcg tctcgcgaga ggacgcatcg agctcgggat gtcggtgcag
     5221 ctccgcgacg cgggggcgcc ggcggtcgag ttgaatctcg acctcgcgaa ggctctttcg
     5281 gccgccgtgg atcgggcccg ggagctgggg ctcgtggccg gcgcgctcac gcctggagac
     5341 ctgctccggc tgccgcaggt cattgccata cgcgagcacg ccgccgagcc ggacgaggcg
     5401 ctcgacgcac gggtcgcggc cagcgtcgag accgccgtgg acgaggcgct cgccgatctc
     5461 gacgcgatgc gcgtgcgtga gggcgctcac cttgccgccg acctcgagtc gaggcgcgag
     5521 cggctgggcg gactgatcga gcagctgtgc gtggcggcag ccgagggaca gcaggcgctg
     5581 gaagcgcggc ttcgggatcg cgtggacgag ctgcgcctgg agttgccggt ggaccaggcg
     5641 atgatcgcgc aggagatcgt gcggctggcc gcccgatccg acatctccga ggaggtgacg
     5701 cggtttcgcg cgcacctggc gcactggcgt gcgctgtcgg agagcgacga gccgtgcggc
     5761 cggaagctcg acttcctgct ccaggagatg aaccgcgaag tcaacacgat cggttcgaag
     5821 gcggacggca tcggcgtctc ggagatcgtc atcgacgcga aagccgagct cgagcgcatg
     5881 cgcgagcagg tgcagaatgt cgaatagcct gccgggcgcc ggtcggctct ggcggggaca
     5941 actgttcatc gtctccgcgc catccggcac cggcaagacc acgctcgtcg aacggctcgt
     6001 gcaacgcgtg gcagggctgc gcatgtcgcg ctcgtacacg tcgaggcccg cccggggggg
     6061 agagcaggac ggggtcgact ataatttcat tacacgcggt cgcttcgagg acatggtccg
     6121 ggagggcgcc ttcctcgaat gggccgatgt cttcggcaat tactacggga cgagcgcgtc
     6181 cgacaccgac gcgatgctcg cgcgcgggga agacgtcgtc ctcgtgatcg acgtccaggg
     6241 cgcgcggcag gtccgggccc gcggcatcca gacgattggc gtgttcgtgc tgcccccgtc
     6301 ggcggacgtg ctcgagcagc ggctgcgggg ccgaagcaag gacagcgaag agcagatcag
     6361 gcggcgtctc gaggtcgccg gtgccgaggt ggacgagtac gcgatgtacg actacgtcgt
     6421 gatcaacgac gagctcgacg cggccgtgga gcggctgcgc gcgatcgtgg gagccgagcg
     6481 ggtgcggacg aggcgcatgc gcgcggagat acaagccatc atcgagacct ttcattcatg
     6541 aagagcacac ccaaagagaa ccggttcgag ttcgtggtca tggccagcgc gcgtgcgcgc
     6601 cagctcctca agggcgcggt gccccgcgag gccggcgaca agaaagtcac gatcgcccag
     6661 cgggagatcc tcaggcgcaa ggtggagaag atcgaagagc gaatagtgga gaagctcgaa
     6721 gagcggccgt aggcaggcac agccatgtcc ctcatcgccc tcggcgtcac cggcggcatc
     6781 ggcgcctaca aggcggtcga ggtctgtcga ggactgcagc agcgggggca cgacgtcgtg
     6841 gcggtcatga cccgctcggc cacccggttc gtcggtccga tgaccttcga ggcgatcacg
     6901 cggcacgcgg tgatcaccga tcaatgggca ccgggcctga acgccgagat cgagcacatc
     6961 aagctgacgg agcaggcgtc gctgctgctc gtcgtgccgg ccaccgcgaa tctgatcggc
     7021 aaactcgcca acggcatcgc cgacgacttc ctgacgtcgc tctacctcgc caccagggcg
     7081 cccgtcctgg tggccccggc gatgaacacc aacatgctcg gtca
//
LOCUS       DBNGSH010000840         2236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2985485, whole genome shotgun sequence.
ACCESSION   DBNGSH010000840 DBNGSH010000000
VERSION     DBNGSH010000840.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2985485"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..874)
                     /locus_tag="AC16CY_19315"
     CDS             complement(59..874)
                     /locus_tag="AC16CY_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356008.1"
                     /translation="MRTWVIAASVVWAGVGLTVSPSAQSADVDAFRKTIEPIFMRDRG
                     GTMPGYAACVMCHTWQTSVRFDLETPATDAGWTGEQSQRNFEMITQLLNTRTPENSWL
                     LLKPLEPKAGGLGHTGGTYWSSREDPEYQTILKWIRSLPPDRYVPKAAPAVDFEFFRS
                     CVQNVFTTPREGHIKCSNCHGAGGLMGFAPVPAEGRTAWNEEEARQAFRVISRLIVAG
                     NPTQSRFLLKPLHPDGGGSYAHNGPRRWQSRDDPEWQMLAAWVRGERKGGRCS"
     gene            complement(880..2037)
                     /locus_tag="AC16CY_19320"
     CDS             complement(880..2037)
                     /locus_tag="AC16CY_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3356009.1"
                     /translation="MASSRLFRTLPAVAGALCLAATVAAGQGVPRLDSYKGVAVRILQ
                     SNNAGNIHHIIDPATNRVTGLIRGCPNPHNLTTHPDGLYYYCSNEAEDTVDVFDTKTL
                     KLVTQIPLSGNPNKIVVNKKHKKIYAGIRAWTADKRAVVDVIDIATHKLVRSVAVHHP
                     VHNTYVTPDDNFVIAGLSGGVRPGEPTIQVIDPVKDQVVWGMELTGYQQYGRNNHEVR
                     PMAFEANADGSTKRMFAQATGINAVWVIDWNTHRVVDMLWPPKLPLWKQNADGIQTGD
                     MHGVEVLPDKSALWASSRLDSRIYGWTLPDLKYIGAVEVGPTANWMTPTPDSKFMYVA
                     ISGSDHTVAVDLKKLAIVAKITTGARPARISTAILPPDRVNAPENSSTRRE"
BASE COUNT          360 a          727 c          715 g          434 t
ORIGIN      
        1 cattcggagc gcagggcccc gccgcacacg cccccggcat gcgggaaagc gaacactgtt
       61 acgagcagcg tcccccttta cgctcgccgc ggacccaggc ggccagcatc tgccattcgg
      121 gatcgtcccg gctctgccag cgtcgaggtc cgttgtgcgc gtacgacccg ccgccatccg
      181 ggtggagcgg cttcaggagg aaccggctct gcgtgggatt gccggcgacg atcagccgcg
      241 agatcacgcg gaacgcctga cgcgcctctt cttcgttcca ggcggtgcgc ccctcggcgg
      301 gaaccggtgc gaaacccatg aggccgccgg caccgtggca gttgctgcac ttgatgtgac
      361 cctcgcgggg cgttgtgaac acgttctgca cgcacgaacg gaagaactcg aaatccaccg
      421 ctggagccgc cttcggaacg tagcgatcgg gcgggaggct cctgatccat ttcaggatgg
      481 tctgatactc ggggtcctcg cgcgagctcc agtacgttcc gcccgtgtgc cccaacccac
      541 cggcttttgg ctcgagcggc ttcagcagca gccagctgtt ctccggagtg cgcgtgttca
      601 ggagctgcgt gatcatctcg aagttgcgct gtgactgctc gccagtccac ccggcgtcag
      661 ttgccggcgt ctcgagatcg aacctgacgc tcgtctgcca ggtgtgacac atcacacagg
      721 cggcgtagcc gggcatcgtg ccgccccggt cgcgcatgaa gatcggttcg atggtcttgc
      781 ggaaggcgtc gacgtcggcc gattgcgccg acggagagac cgtcagcccg acgcctgccc
      841 acaccacgct cgcggcaatc acccatgtgc gcatggctgc tactcccggc gcgtgctgga
      901 gttctccgga gcgttgacgc gatcgggcgg cagaatcgcc gtgctgattc gcgccggcct
      961 cgcgccggtc gtgatcttgg cgacaattgc cagcttcttc aggtccaccg ccactgtatg
     1021 gtcggatccg gagatggcga cgtacatgaa cttgctgtca ggagtcggcg tcatccagtt
     1081 ggccgtcgga ccgacttcca ccgcaccgat gtatttgaga tccggcagcg tccagccgta
     1141 gatgcggctg tcgaggcggc tcgatgccca gagggccgac ttgtcgggca gcacttcgac
     1201 gccgtgcatg tcgccggtct ggatcccgtc ggcattctgc ttccagagcg gcagcttcgg
     1261 aggccagagc atgtcaacca cccgatgcgt gttccagtcg atcacccaga cggcgttgat
     1321 gccggtcgcc tgcgcgaaca tccgcttggt cgagccatcc gcgttggcct cgaaggccat
     1381 tggccgcacc tcgtgattgt tgcggccgta ttgctgatac cccgtcaatt ccatccccca
     1441 cacaacctgg tccttgacgg gatcgatcac ctggatcgtc ggttcccctg gccgcacccc
     1501 gccggagaga ccggcgatga cgaagttgtc atccggcgtc acgtaggtgt tgtgcaccgg
     1561 gtgatgaacg gcgacgctgc gaacgagctt gtgtgtggcg atgtcaatca cgtccacgac
     1621 cgcgcgcttg tcagcggtcc aggctcggat acctgcatag atcttcttgt gcttcttgtt
     1681 caccacgatc ttgttgggat tgcctgagag cgggatctgc gtcacgagct tcagcgtctt
     1741 cgtgtcgaac acgtcgacgg tatcctccgc ctcgttcgag cagtagtagt agaggccatc
     1801 cgggtgagtg gtcaagttgt gcgggttcgg acagcccctg atgagtcccg tcaccctgtt
     1861 cgtggccgga tcgatgatgt gatggatgtt tccggcgttg ttgctctgga ggatccgtac
     1921 ggcgacgccc ttataggagt cgagtcgcgg gactccctgg ccggcggcca ccgtcgccgc
     1981 caggcacagc gcgcccgcaa cagccgggag ggttcggaac aagcgtgaag aagccatcgt
     2041 cgacctccat gaccgacctg agggttccgg cggccctcac aataccaagg tccggggatt
     2101 cgcggtagtc cttgctacta tgacgcgatg ccacgagtga ggcgcaccgc cgtggccggc
     2161 cgctggtatc cggacgatcc cgtgccgctt gccgccgaac tcgacgtcat gctggcgagc
     2221 gctcacgtcg agccgg
//
LOCUS       DBNGSH010000841         2651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1458012, whole genome shotgun sequence.
ACCESSION   DBNGSH010000841 DBNGSH010000000
VERSION     DBNGSH010000841.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1458012"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /gene="ccsA"
                     /locus_tag="AC16CY_19325"
     CDS             complement(<1..590)
                     /gene="ccsA"
                     /locus_tag="AC16CY_19325"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR3356010.1"
                     /translation="MPTWFTPAALVCGLLFAASPLVIATAPYESTMGLVQKIFYYHMP
                     SAWMFLIASIVCGVSSVRFLATGDARHDRTAWAAAEMTVLFGLVTLVTGPLWARKAWG
                     TWWVWDARITSSLVGWMIAWAYLILRKYGGAGSEKLAAGLALFGMANVPFIYISVNYW
                     RTIHPATTVVPRLPVSMGFPLWYCVIAFLLLFVLLL"
     gene            complement(692..1366)
                     /locus_tag="AC16CY_19330"
     CDS             complement(692..1366)
                     /locus_tag="AC16CY_19330"
                     /inference="COORDINATES: protein motif:HMM:NF015344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR3356011.1"
                     /translation="MLRAIWLIVRKDLTVEVRSREIAYTTLFFAVSCVLVFAFSLVRE
                     GRAAEGVGAGVLWLAILFAGNLALGRTFERERQAETLRALMLAPASRPAVYLGKLTGI
                     VVLLAGTEIVLVPLVALLFEARLLAHPYWLTAVVATGTIGFAAVGTLFAAMLVRARSR
                     DVLLPVLLYPLTVPVVIAGVRATMALAQPDFDAAIVRFWLMLLVSFDVVFVTLALWTF
                     EPVMTD"
     gene            complement(1366..2070)
                     /locus_tag="AC16CY_19335"
     CDS             complement(1366..2070)
                     /locus_tag="AC16CY_19335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3356012.1"
                     /translation="MTSVDFDEISADDVSRHFGRRRAVSHVSFQAARGTILGLLGPNG
                     AGKSTLLAMAATSMRPSSGAFRYGRLDSSAGAGLRARIGVLGHDQFLYPELTAHENLA
                     FFAGLYGLADPDRRADAALEAAGLATRRNDPVSTLSRGMRQRVALERALIHEPRLALL
                     DEPFTGLDDASADALLGRLKRLRDSGAIVIVATHDLDLAEGLLDRAMVLRDGRALETL
                     AKPEGLRTAYRRVVST"
     gene            complement(2067..>2651)
                     /locus_tag="AC16CY_19340"
     CDS             complement(2067..>2651)
                     /locus_tag="AC16CY_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356013.1"
                     /translation="VTIAGPFASGTTAVQVAYRIRSSDSDLTISQTWPAALPQVIVGV
                     QKIGGLRVSSPQIETMNDVRTDNGDLFVLGNGPAVPAGGTVTVTLSGLPAQSQVPRYV
                     ALALAASVIGLGIWLSLSGRERRDEGRQALVARRETLLGQLAQMEAKRRSGAVEADAQ
                     VRRRSRILAELEQIYGELDETHPAPQGGGEGVAA"
BASE COUNT          495 a          922 c          877 g          357 t
ORIGIN      
        1 ttgaggagca gcacgaacag cagcaggaat gcaatgacgc agtaccagag cggaaacccc
       61 atcgacaccg gcagcctggg cacgaccgtc gtcgccgggt gaatcgttct ccagtagttg
      121 acggagatgt agatgaaggg cacgttcgcc atcccgaaca gcgccaggcc ggccgcaagc
      181 ttctccgacc cggccccgcc gtacttgcgc aggatgagat acgcccacgc gatcatccac
      241 ccgacgaggc tcgacgtgat gcgcgcgtcc cagacccacc acgtccccca cgccttccgc
      301 gcccagagcg gtccggtcac cagcgtgacc aggccgaaga gcaccgtcat ctccgcggcc
      361 gcccatgccg tccggtcgtg cctggcgtcg cccgtcgcaa gaaagcgcac gctcgacacg
      421 ccgcacacga tgctcgcaat caggaacatc caggccgacg gcatgtggta gtagaagatc
      481 ttctgcacca gccccatcgt ggactcgtag ggcgccgtgg cgatcaccag gggtgatgcg
      541 gcgaacagca gcccgcagac aagggcggcc ggagtgaacc aggtaggcat agggatcacg
      601 gaatactatc gcaactccca acgaccaact cccaacgccc aaagaacgtg cgaaccattg
      661 ggagttggag attgggaatt gggagttgac ctcagtccgt catcacaggc tcgaacgtcc
      721 agagcgcaag cgtcacgaac acgacgtcga acgacacgag cagcatcagc cagaaccgga
      781 cgattgccgc gtcgaagtcc ggctgggcca gcgccatcgt ggcccggacg ccggcgatga
      841 cgaccggtac cgtgagcgga tagagcaaca ccggcaggag cacatcgcgg ctccgggcgc
      901 gcacgagcat cgccgcgaac agcgtgccca cggcggcgaa cccgatcgtt ccggtggcga
      961 cgacggccgt gagccagtac gggtgagcca gcagccgggc ctcgaacagc agcgcgacca
     1021 gggggacgag cacgatctcg gtacccgcga gcagcacgac gatgccggtc agcttgccca
     1081 ggtagacggc gggccgcgat gccggggcga gcatcaacgc gcgcagcgtc tcggcctggc
     1141 gctcgcgctc gaaggtccgg cccagtgcga ggttgcccgc aaacaggatc gccagccaca
     1201 gcacgccggc gccgacgcct tccgctgctc tcccctctcg caccagcgaa aacgcgaaca
     1261 ccagcacgca cgacacggcg aagaagagcg ttgtgtacgc aatctcgcgg ctccgtactt
     1321 cgacggtcag atctttcctg acgatcagcc agatcgcgcg aagcatcagg tgctcacgac
     1381 tcgccgatac gcggtccgga gtccctcggg cttcgccagc gtctccaggg cacggccatc
     1441 gcgcagcacc atggcgcgat cgaggagccc ctcggccaga tccagatcgt gcgtggcgac
     1501 gatgacgatc gctccgctgt cccggagccg cttgagcctt ccgaggaggg cgtccgcaga
     1561 ggcatcgtcg agtcccgtga agggttcgtc gagcagggcc agccgcggct cgtggatgag
     1621 ggcgcgctcg agcgcgacgc gctggcgcat gccccgcgac aaggttgaca cagggtcgtt
     1681 gcggcgggtg gcaaggccgg ctgcctcgag cgccgcgtca gcccggcgat ccggatccgc
     1741 gaggccgtag agcccggcga aaaacgcgag gttctcgtgc gccgtcagtt cggggtagag
     1801 gaattgatcg tgtcccagga cgccgatgcg ggcacgaagt ccggcgccgg ccgaactatc
     1861 caggcgtccg taacgaaacg ccccggacga gggacgcatc gacgtcgccg ccatcgccag
     1921 cagcgtggac ttccccgcgc cgttcgggcc gagcaggccc aggatcgtgc cgcgcgcggc
     1981 ctggaagctg acgtgggata cggcgcgccg cctgccgaaa tggcgggaca cgtcgtcggc
     2041 cgagatctcg tcgaagtcga cgctcgtcac gcggcgacgc cctcgccgcc tccttgcgga
     2101 gcagggtgag tctcgtccag ctcgccgtag atctgttcga gctccgccag gattcggctg
     2161 cggcgccgga cctgcgcgtc cgcctcgacc gcgcccgaac ggcgcttggc ctccatctgc
     2221 gcgagctggc cgagcagggt ctcgcggcgc gcgacgagcg cctgccggcc ctcatcccgc
     2281 ctttcccggc cgctgagcga cagccagatc ccgaggccga tcacgctcgc cgccagagcc
     2341 agcgcgacgt accgaggcac ctggctttgt gcgggaagac cagagagcgt caccgtcacc
     2401 gtaccgcccg ccggcaccgc cggtccgttg ccgagcacga acaggtcgcc gttgtccgtg
     2461 cggacgtcgt tcatcgtctc gatctgggga gacgacacgc gaagcccgcc aatcttctga
     2521 acaccgacga tgacctgcgg gagcgctgcc ggccaggtct gcgagatcgt gaggtcgctg
     2581 tccgagctgc gtattcggta cgcgacctgc acggcggtcg tgccggacgc gaacggaccg
     2641 gcaatcgtca c
//
LOCUS       DBNGSH010000842         7178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4005390, whole genome shotgun sequence.
ACCESSION   DBNGSH010000842 DBNGSH010000000
VERSION     DBNGSH010000842.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4005390"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1592)
                     /locus_tag="AC16CY_19345"
     CDS             complement(6..1592)
                     /locus_tag="AC16CY_19345"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3356014.1"
                     /translation="MRTIVSYVCLVVVVVAVFGTTASVFAQEAKPAGQPAAQAGLLTR
                     RLTAAEAVALALENNLGIKAARIDPEIEAINLVGARAGWLPTFSASLQTSSNEAPNQN
                     FLSGALGGSTTSDNFGNELGVQQTLQWGGSYNLGWDSSRFNSNSSYSTFSPQLRSSLS
                     LNYTQPLLRNFSIDNIRQQVQVTTKNQEITDVGLQQTIAQMTRTVRNAHWELAYTIAS
                     LGVQQQSLDLAQESLRNTRARVEIGTTPPIDVVAAEAEVARRREAVIVAEAQIETAQD
                     NLRTLVFDPEMPDFWAIRIEPGELPPFQPTVVNVDSAVRNALERRTDLMQARKSLEVD
                     DINIKYFRNQTLPDVNASFNYGLQGLGGTQLQRGIGPFGPGSGDVLSESQRGFGTVLS
                     DLFANEYPNWTFAVNVGYPLGTSSADASLARARLQQNQSRTQLRNLELQIAAQVRQAA
                     RQVQTLEQRVASSRSARELAERTLAAEERKFEAGTSTNFLVTQAQRDLSLARNVELQN
                     VLEYNRAVVDLETVQDVPLR"
     gene            1689..2267
                     /gene="pyrE"
                     /locus_tag="AC16CY_19350"
     CDS             1689..2267
                     /gene="pyrE"
                     /locus_tag="AC16CY_19350"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR3356015.1"
                     /translation="MPSVTSIPVLDRFRRVGALLEGHFRLTSGLHSPGYLQCALVLQH
                     PQEAEACGAEIAAAAGDLEPQVVLSPALGGIVIGQEVARALGVRAIFAERQDGTLMLR
                     RGFSLAPGERVLVVEDVVTTGGSTRETIDVARASGAVVVGAAAIVDRSGGLRDLDVPF
                     RALAEISLPTYQPDNCPLCAAGQPVVKPGSRV"
     gene            2360..3097
                     /gene="truA"
                     /locus_tag="AC16CY_19355"
     CDS             2360..3097
                     /gene="truA"
                     /locus_tag="AC16CY_19355"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3356016.1"
                     /translation="MRTLKLTLAYDGTRLVGWQRQADGVSVQGVLEDALSLLEGARVS
                     VHGAGRTDAGVHAIGQVASVTVGCSHDAATLQRALNAQLPDDVRVLAIEEVPSDFHAR
                     FSARSKTYRYRIRNAPIADPFDRLYTWHVPETLDVDAMRRAAASLVGTHDFAAFQSTG
                     SDTTSTIRTITRSEWQEAPGVLTYEIAGTGFLRHMVRAIVGTLVEVGRGWRQPASVAA
                     LLAGGSRSESGPTAPALGLFLVDVDYH"
     gene            3124..3912
                     /locus_tag="AC16CY_19360"
     CDS             3124..3912
                     /locus_tag="AC16CY_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR3356017.1"
                     /translation="MPLDNLLAWIPPGHPDAMLAREIHFDRLPVHVAIIMDGNGRWAA
                     QRHLPRVEGHRAGIDAVRDSVETAARLGIRVLTLYAFSIENWKRPASEVGTLMQLLKR
                     YLRSELNTLLSNDIRFRVIGRMEDLAPDIKDELNAAIERTSTSSGMLFNIALNYGGRA
                     EIVDAARQAIASGVSPQDLDEERFASFLYTAGQPDPDLLIRTSGEMRVSNYLLWQIAY
                     SEIYVTDTLWPDFRRRHFIEAVLAYQKRERRYGGISSPAAIGAK"
     gene            3923..4681
                     /locus_tag="AC16CY_19365"
     CDS             3923..4681
                     /locus_tag="AC16CY_19365"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3356018.1"
                     /translation="MVRFASGVALAAVALAAILFLPLAALRVLACLVAALAAYEYLEL
                     AKTPIRAVVLAVALCWVSSAPEPPSAYVLLSMALLWAGAEVLLANHSLEQATTGIFGA
                     VYVGMPLGLLVAVHALQGWRATLLLVGTVVVSDTAQYYTGRSLGRRPLAPSISPHKTI
                     EGAIGGMVAGPALMALVGPSALPSASAGPLLVVGLLVVGLGICGDLFESRLKRAAGAK
                     DSSSLIPGHGGVLDRIDALLFAVAPFYLYVRNIS"
     gene            4678..5847
                     /locus_tag="AC16CY_19370"
     CDS             4678..5847
                     /locus_tag="AC16CY_19370"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR3356019.1"
                     /translation="MKSLAILGSTGSIGRSALAVVDAHPARLRVVALAAGDNADLLAA
                     QVQRYRPELAALATSGAVDRLRAACETAVPMRVAVGAEGLVEVATHPSADIVICASTG
                     TTAIEAVLAAVEAGKTIALANKEVLVTAGALVTEAARRKGVAILPVDSEHNAVHQCLH
                     GRSRTELRRLILTASGGPFRSLSREELDRVGADEALRHPTWKMGRKITIDSATLMNKG
                     LEVIEAHWLFGVSADQIDVLIHPQSIVHSLVELNDGSVIAQLGVTDMRLPIQYACSYP
                     ERWEAALPSLDLTRAGHLEFEAPPHDRFPCLNLAYAALRAGGTLPVVLNSANEVAVEA
                     FLDGKLGFTSIPTLIERTMNAHASESVSSVQTVRRVDAWARQYASEVAGELELTI"
     gene            5861..>7178
                     /gene="rseP"
                     /locus_tag="AC16CY_19375"
     CDS             5861..>7178
                     /gene="rseP"
                     /locus_tag="AC16CY_19375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR3356020.1"
                     /translation="MLTILAFLFVLGVLIFVHELGHFLAARRHGVRVLTFSLGFGPKI
                     LKATRGDTEYCISAIPLGGYVKMAGENPDDPRSGQPDEFLSKTKWQRLQIYVAGPAMN
                     IILAVIVLAFVLAQGAEREVYRDEPPVIGTVLPDSPAARAGLRRNDRIVAVAGRNVAT
                     WQDLFVAIGTRPDRDVGLTYTRDGKSIDVTVRPVGQGEYEIGNIGVLPDIHPVVASLI
                     PGEHAEAAGFKAGDVIVAVNGERMVTRNQLIELTARSAGRTLEFSILRDGKALTLTGT
                     PQLRGSKGMMGFYLGEPTKMFKPGPLTALRLSVERNVELSGLLFRILGGLFTGETSIR
                     DFQGPVGIGRLSGEAAQEGFVSLLSLMALLSLQLGIINLMPVPILDGGHILIMGLEGI
                     ARRDFSMQVKEKMLLAGFVVLMMLMVTVIYNDLTRISWIERFMPWRN"
BASE COUNT         1125 a         2347 c         2421 g         1285 t
ORIGIN      
        1 tgatgttatc gcaacggcac gtcctggacg gtctccagat ccaccaccgc gcggttgtat
       61 tcgagcacgt tctgaagctc cacgttccgc gccagggaca ggtcgcgctg cgcctgagtc
      121 acgaggaagt tcgtggaggt gccagcctcg aacttgcgct cctccgccgc gagcgtgcgc
      181 tcggccagct cgcgcgccga cctcgagctc gccacccgct gctccaaggt ctgcacctgg
      241 cgcgccgcct ggcgcacctg cgcggcgatc tgcagctcca ggttgcggag ctgcgtgcgc
      301 gactgattct gctgcagcct ggctcgcgcc agcgacgcgt ccgccgaact cgttccgagc
      361 ggatacccga cgttcaccgc gaacgtccag ttgggatact cgttggcgaa caaatcggac
      421 agcacggtcc caaagccgcg ttgagactcg ctgaggacgt cgcccgagcc cggcccgaac
      481 ggaccgatgc cgcgctggag ctgtgtgccg cccagaccct gaaggccgta gttgaagctg
      541 gcattcacgt ccgggagggt ctggttgcgg aagtacttga tgttgatgtc gtccacttcg
      601 agcgactttc gcgcctgcat caggtcggtt cgccgctcga gcgcgttccg cacggcggag
      661 tccacattga cgaccgtcgg ttgaaacggt ggcagttccc ccggctcgat cctgatcgcc
      721 cagaagtcgg gcatctcggg atcgaagacg agcgtgcgca ggttgtcctg cgccgtctcg
      781 atctgcgctt ccgcaacgat caccgcttca cgacgccgcg caacttcggc ctcggccgcg
      841 accacgtcga ttggcggcgt cgtcccgatc tcgacgcggg cacgcgtgtt ccgcagcgac
      901 tcctgcgcca gatccagcga ctgctgctgc acgccgagcg aggcgatggt gtaggcgagt
      961 tcccagtgtg cgttccgcac ggtccgtgtc atctgcgcga tcgtctgctg cagccccacg
     1021 tcggtgatct cctggttctt ggttgtgacc tgcacctgct gacggatgtt gtcgatgctg
     1081 aaattgcgga gcaagggctg cgtgtagttc agcgagagcg aggagcgaag ctggggcgag
     1141 aacgtcgagt acgagctgtt gctgttgaag cgggagctgt cccagccgag gttgtagctg
     1201 ccgccccact ggagcgtttg ctggacgcca agctcgttgc cgaaattgtc gctggtcgtc
     1261 gatcctccca gcgctcccga aagaaagttc tggtttggag cctcgttgct gctggtctgc
     1321 agcgacgcac tgaatgtggg gagccagccg gcgcgagcgc ccacgaggtt gatcgcctcg
     1381 atctccggat cgatgcgggc agccttgatc ccgaggttgt tctcgagggc cagcgccacc
     1441 gcttcggcag ccgtcaggcg cctggtcagc agcccggctt gagccgccgg ctgaccggcg
     1501 ggcttcgcct cctgtgcgaa aacggacgct gtcgttccga aaacggccac gacaaccacg
     1561 accaggcaca cgtaacttac gatcgtacgc atctttccac tccccaaagc ttttgtgatt
     1621 ccaacctcaa gtaagacgaa cgcccccggg cagaggttct gacagtgcaa aaggtatact
     1681 cggccgaaat gccctcggtg accagtattc ccgttctcga caggttccgc cgcgtcgggg
     1741 ccctgctcga gggccacttt cgcctgacat ccgggctcca cagccctggc tatcttcaat
     1801 gcgcgctcgt gctgcagcat ccgcaggaag ccgaggcctg cggggccgag attgccgccg
     1861 cggccggcga tctcgagcct caggtcgtgt tgtcccccgc cctcggcggc atcgtcatag
     1921 ggcaggaagt cgcgcgggcg cttggagtac gggcgatttt cgcggaacgt caggacggca
     1981 cgctgatgtt gcggcgcggg ttttcactcg cgccgggaga acgggtgctg gtggtcgagg
     2041 atgtcgtcac gacgggcggt tccacccgcg agacgatcga cgtcgcgcgg gcctcggggg
     2101 ctgtcgtcgt cggggcggcc gccatcgtgg acagaagcgg tggcctgcgg gacctggacg
     2161 taccatttcg tgcactggcc gaaatatccc ttccgaccta ccaaccggac aactgtccac
     2221 tctgtgccgc cgggcagccg gtggtgaagc cggggtcacg tgtctgaaac tcccacatcc
     2281 caactcccaa ttcccaagca agcctatcgc gttgggggtt ggaagttggg agttgggagt
     2341 tgactgagtt gcagcccccg tgcgcacgct gaagctgact ctcgcctacg atgggactcg
     2401 cctcgtgggt tggcagaggc aggccgatgg cgtgtcggtg caaggcgtgc tcgaggacgc
     2461 gctgtcgctc ctcgaaggcg caagagtaag cgttcacggc gcggggcgca cggacgccgg
     2521 cgtccacgcg atcggccagg tggccagcgt gaccgtgggc tgttcccatg acgcagccac
     2581 gttgcaacgc gcgctgaacg cgcagctccc cgacgacgtg cgcgtgctcg cgatcgagga
     2641 ggtcccctcg gacttccacg cgcggttcag cgcgcggtcg aagacctacc ggtaccggat
     2701 caggaacgcg ccgatcgccg acccgttcga ccggctctac acgtggcacg tacccgagac
     2761 gctcgacgtc gatgccatgc ggcgtgccgc ggcgagcctg gtcgggacgc acgacttcgc
     2821 ggcttttcag agcaccggca gcgacaccac gagcacgatt cgaacgatca cgcgatcgga
     2881 gtggcaggag gcgcccggcg tgctcaccta cgagatcgcg ggcaccggct tccttcgcca
     2941 catggtgcgc gccatcgtgg gcacgctcgt cgaggtcggg cgaggatggc gtcagcccgc
     3001 gagcgtagcg gcgcttctcg cgggagggtc gcgatcggag tcgggcccga cggccccggc
     3061 gctcggactg ttcctcgtcg atgtggatta tcattaaccc gttgtcagac gaggaattga
     3121 gctgtgccgc ttgacaatct gctcgcgtgg attccgccag ggcatcccga tgccatgctc
     3181 gcccgggaga tccacttcga tcggcttccc gtccatgtcg cgatcatcat ggatggcaac
     3241 gggcgctggg cggcccagcg tcacctgccg cgcgtcgagg gccatcgggc cggcatcgac
     3301 gcggtgcgcg attcggtcga aaccgccgca cggctcggca tccgggttct cacgctgtac
     3361 gccttctcga tcgaaaactg gaagcggcct gcgagcgaag tgggcacgct catgcagctg
     3421 ctcaagcggt acctgaggtc cgagctgaac acgctgctca gcaacgacat ccgcttccgc
     3481 gtgatcggac ggatggagga tctggctccc gacatcaagg acgagctgaa cgcggcaatc
     3541 gagcgaacgt cgacgagcag cggcatgctg ttcaacatcg cgttgaacta cggcggccgc
     3601 gccgagatcg tggacgccgc gcgacaggcg attgcctccg gcgtgtcacc acaggatctc
     3661 gacgaggagc gattcgcgag tttcctctac actgcgggac aacccgaccc ggacctgctg
     3721 attcggacga gcggcgagat gcgggtcagc aactacctgc tctggcagat tgcctactcg
     3781 gagatctacg tgaccgatac gctgtggccg gacttccggc gccgtcactt catcgaggcc
     3841 gtgctggcgt atcagaagcg tgagcggcgg tacggcggca tcagctcgcc ggcggcgatc
     3901 ggggcgaaat gaagcgcgcc tgatggttcg ctttgccagc ggggtggctc tcgcggcagt
     3961 tgcgctcgca gcgatcctgt tccttcccct cgccgcgcta cgcgtgctgg catgtctcgt
     4021 cgcggcgctg gcggcgtacg agtacctcga gctcgcgaag acgcccattc gcgcggtcgt
     4081 gctggccgtc gcgctgtgct gggtgtcgtc ggcgccggag ccgccgtctg cgtacgtact
     4141 cctgtcgatg gccctgctat gggccggggc agaggtactg ctggccaatc actcgctcga
     4201 acaggccacg accgggattt tcggagccgt ttacgttgga atgcctctcg gcttgctggt
     4261 ggccgttcac gccctgcaag gctggcgcgc gacgctgctc ctggtcggta cggtcgtggt
     4321 gagcgatacg gctcagtact acaccggccg gtcgctgggc cggaggccgc tggctccttc
     4381 gatcagcccg cacaagacca tcgaaggcgc catcggggga atggtggccg gtcccgcgtt
     4441 gatggcgctg gtcgggccgt cagctctgcc gtccgcgagc gccggaccgc tgctcgtggt
     4501 cgggctgctc gtcgtagggt tgggaatctg cggcgatctc ttcgagtcgc ggctcaagcg
     4561 cgccgcgggc gcgaaggaca gctcgtcgct cattcccgga cacggcggcg tgctcgatcg
     4621 catcgacgcg ctgctgttcg ccgtcgcgcc gttctacctg tacgttcgaa acatctcgtg
     4681 aagagcctcg ccattctcgg ttcgacgggt tcgatcggtc gaagcgccct cgcggtcgtg
     4741 gatgcgcatc ccgcgcggct gcgggtggtc gcgctggcgg ccggcgacaa cgccgatctg
     4801 ctggcggcgc aagtgcagcg ctatcggccg gagctcgcgg ccctggccac ctccggcgcc
     4861 gtggaccggc tgcgcgccgc gtgcgagacg gcggtaccga tgcgggtcgc cgtgggcgcc
     4921 gagggcctcg tcgaggtcgc gacgcatccg tccgccgaca tcgtcatttg cgcctcgacc
     4981 ggcacgaccg cgatcgaagc cgtgctcgcc gccgtcgagg cgggcaagac gatcgcgctc
     5041 gccaacaagg aggtgctcgt caccgcgggg gcgctcgtga ccgaggcggc gcggcgcaag
     5101 ggggtcgcca tcctgcccgt agacagcgag cacaacgccg tgcaccagtg cctgcacggc
     5161 cgatctcgca ccgagctccg gcgattgatc ctgacggcgt cgggcggacc gttccggagc
     5221 ctctcgcgcg aggaactcga tcgcgtcggc gcggacgagg cgctgcggca tcccacctgg
     5281 aagatggggc gcaagatcac gatcgattcg gccacgctga tgaacaaggg cctcgaggtc
     5341 atcgaggcgc actggctgtt cggcgtctcc gccgatcaga tcgacgtcct catccacccg
     5401 cagtcgatcg tccactcgct ggtggagctc aacgacggct cggtgatcgc gcagctcggc
     5461 gtgaccgaca tgcgcctgcc gatccagtac gcgtgttcgt atccggagcg gtgggaggcg
     5521 gcgctgccgt cgctcgatct gacacgcgcc ggtcatctcg agttcgaggc cccgcctcac
     5581 gatcgcttcc cctgcctgaa cctcgcctac gccgcgctcc gggcgggagg cacgcttccc
     5641 gtcgtgctga actccgccaa cgaagtcgcg gtggaggcgt ttctggatgg aaaactcggc
     5701 tttacgtcga ttcccacgct catcgagcgg acgatgaacg cccatgcctc cgagagcgtc
     5761 tcgtccgtgc aaaccgtgcg ccgggtcgat gcgtgggccc ggcaatacgc cagcgaggtc
     5821 gcgggcgagt tagaattgac gatctagggc tacgggcgct gtgctgacca tcctggcgtt
     5881 tctcttcgtt ctgggcgtgc tgatcttcgt tcacgagctg gggcacttcc ttgccgcgcg
     5941 tcgtcacgga gtgcgcgtgc tcacgttctc gctcgggttt ggcccgaaga tcctcaaggc
     6001 gacccgcggc gacaccgagt actgcatcag cgcgattccg cttggcggct acgtgaagat
     6061 ggccggcgag aaccccgacg atccccgctc cgggcagccc gacgaattcc tctcgaagac
     6121 gaagtggcag cgcctgcaga tctacgtcgc cggcccggcg atgaacatca tcctggcggt
     6181 catcgtgctc gccttcgtgc tcgcccaggg agcggagagg gaagtctacc gggacgagcc
     6241 gccagtcatc ggcacggtgc tgcccgattc gccggccgcg cgggcgggac tgcggcggaa
     6301 cgaccgcatc gtggccgtgg ccggccgaaa cgtcgcgacg tggcaggacc tgttcgtggc
     6361 gatcggcacg cgccccgatc gggacgtcgg attgacgtac accagggatg gcaagtccat
     6421 cgacgtgacg gtgaggcccg ttggccaggg cgagtacgag atcggcaaca tcggcgtgct
     6481 gccggacatt catccggtgg tcgcctcgct gattcccggc gagcatgccg aggccgcggg
     6541 cttcaaggcc ggggacgtga ttgttgccgt caacggcgag cgcatggtga cgcggaatca
     6601 gctgatcgag ctcaccgcgc gcagcgccgg ccgcacgctc gagttctcca tcctccgcga
     6661 cgggaaagcg ttgacgctga cgggcacgcc gcagttgcgc gggtcgaaag gcatgatggg
     6721 cttctacctc ggggagccca ccaagatgtt caagcccgga ccgttgacgg ccctccgcct
     6781 gagcgtggag cgcaacgtcg agctgtccgg gctgctcttc aggatcctgg gcggactgtt
     6841 cacgggcgag acgtccattc gtgatttcca gggccccgtc gggatcggca ggctctcggg
     6901 cgaggcggcg caggagggct tcgtttcgct gctgtcgctg atggcgctgc tcagcctcca
     6961 acttggcatc atcaacctga tgccggtgcc catcctcgat ggcgggcaca tcctgatcat
     7021 ggggctcgag ggcatcgcgc ggcgcgactt cagcatgcag gtgaaagaga agatgctgct
     7081 cgcggggttc gtcgtgctga tgatgctcat ggtgaccgtc atctacaacg acctcacgcg
     7141 catcagctgg atcgaacgct tcatgccctg gcgcaact
//
LOCUS       DBNGSH010000843         3372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3326649, whole genome shotgun sequence.
ACCESSION   DBNGSH010000843 DBNGSH010000000
VERSION     DBNGSH010000843.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3326649"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..966)
                     /gene="rlmB"
                     /locus_tag="AC16CY_19380"
     CDS             complement(232..966)
                     /gene="rlmB"
                     /locus_tag="AC16CY_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR3356021.1"
                     /translation="MIVYGINPVLEAMRAGRVKQLWIGERRDERMRELLAFAATHGIS
                     PQHVPSDALARRARGGVHQGVVADVDDAAQFTVGEIVRGSSDPPLIVVLDGVEDPHNL
                     GAILRTADASGASGVVVQARRSAARSGAAAKASAGAVAHVRVAEVVNIARALEELKDL
                     GVWTVGLSGDAATAYDSIDFTLPTAIVLGAEGEGLRRLVRERCDYLASIPMSGRVGSV
                     NVSVAAGVVLFEAVRQRRQRPKKPGG"
     gene            complement(988..2511)
                     /gene="gltX"
                     /locus_tag="AC16CY_19385"
     CDS             complement(988..2511)
                     /gene="gltX"
                     /locus_tag="AC16CY_19385"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3356022.1"
                     /translation="MTPDPIRLRFAPSPTGQLHVGNARTALFNWLLARGSGGTLILRI
                     EDTDVERSTSESERGILEDLRWMGLDWDEGVERGGTHGPYRQSERLHIYRAHAVELLA
                     GGHAYHCFCPARQLEADREAALAAGRPPKYSGRCRLLTKDEARHRVESGEQAVIRFRV
                     PDARDVSFDDIVRGRVTFNTDVIGDPVLLRSDGSPAYNYAVVIDDALMEITHVIRGED
                     HISNTPRQLLLYEAFGWRPPAFAHVSLVMGPDHSPLSKRHGATSVAEFRARGFLPEAL
                     ANYLALLGWSPGDGRELLPVGELAALFRLEDVGHSAGVFDVEKLAWVNRHYLKSAAPE
                     RLARLAVPYFQREGWIADPTDADVRFLSQIVPVAAASIDRVEQVTARLAFLFDYSASR
                     ALDVPGIRSEAQSSRDVIAALADELGRAGPLLDRDTFRGAAARVRERTGQKGRALFHP
                     IRLALTGEAEGLELDVAVPAIEAGAALQPSGIRRVPSARERAAEFARCVSDLGPPEA"
     gene            complement(2575..>3372)
                     /gene="ispF"
                     /locus_tag="AC16CY_19390"
     CDS             complement(2575..>3372)
                     /gene="ispF"
                     /locus_tag="AC16CY_19390"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR3356023.1"
                     /translation="AASEYGAAIAAVPVPDTVKQVKEGGVIAATLRREEIYLAQTPQA
                     FRVGVLRAALALDGDATDEAMLAERAGHQVRVVEGDLRNLKITTPDDLALAERYLQID
                     ASRHAALRIGNGYDLHRLVPGRLLILGGVTIPFDTGLLGHSDADAVCHAVTDAVLGAA
                     GAGDIGRHFPDTDPAWKDANSLDMLRRAATLVRAAGYAVVNVDLVVIAQRPKLSPYAD
                     QIRANLANALGCDASQIGVKGKTNEGVDSMGAGESIAVHAVALLARP"
BASE COUNT          543 a         1168 c         1146 g          515 t
ORIGIN      
        1 aatcagctgc tctaccggac tgagctacgc cagccagaca aagggcagat aatagcactt
       61 cctgtgccag catggcaaac cgctgcgcac attgtacttg aagcgcacgc aagacgagaa
      121 gctcccctgg gacggacggc ccgcaccgaa ggtccggaga ttggaacccg taacccattg
      181 gcttataggg ccttggccgt cagggcctac gttcgtgccg tcccggcgcg gctagccccc
      241 gggcttcttc gggcgctgtc gtcgctggcg gacggcctcg aacagcacga cgccggcggc
      301 caccgagacg ttcacgcttc ccaccctgcc gctcataggg atcgacgcca ggtagtcgca
      361 tcgctcacgc acgagccgtc tgagtccttc gccttcggcg ccgagcacga tggcggtcgg
      421 cagcgtgaag tcgatcgaat cgtaggccgt ggcggcatcg cccgacaggc caaccgtcca
      481 cacgccgagg tccttcaact cctcgagggc ccttgcgatg ttgaccactt cggcgacccg
      541 gacgtgcgcc accgcgccgg ccgacgcctt cgcggccgcg cccgacctcg cagccgaccg
      601 cctcgcctgg acgacgacgc cgctcgcgcc gcttgcatcg gcggtgcgga ggatcgcccc
      661 gaggttgtga ggatcctcga cgccgtcgag gacgacgatg agcggtggat cgctcgagcc
      721 gcgcacgatc tcgccgacgg tgaactgcgc cgcgtcatcc acgtcggcga ccacgccctg
      781 gtgcacgccg ccccgggcgc ggcgggcgag cgcgtccgac ggaacgtgct gagggctgat
      841 gccgtgcgtg gcggcaaagg cgagcaactc ccgcatgcgt tcgtctcgac gctcgccgat
      901 ccacaactgc ttcacgcgtc cggcgcgcat cgcctcgagg acggggttga tgccgtaaac
      961 gatcatgaag gcctcagggc tcggggctca ggcctcagga ggccccagat cggaaacaca
     1021 tcgtgcgaat tcagcagccc gttcgcgcgc gctcggaaca cgtcggattc ccgacggctg
     1081 cagcgctgct ccggcttcga tggccggcac ggccacgtcc agctcgagcc cttctgcctc
     1141 gccggtgagc gcgagccgga tcggatgaaa gagcgccctc cctttctgcc ctgttcgctc
     1201 gcgaacgcgg gcggcggcgc cgcggaaggt gtcgcggtcg aggagcggcc cggcccggcc
     1261 gagttcgtct gccaacgcgg caatcacgtc ccgcgacgac tgcgcctcgg atcgaatccc
     1321 ggggacgtcg agagcgcgcg aggccgagta gtcgaacagg aacgccagcc tcgcggtcac
     1381 ctgctccacg cgatcgatcg acgccgcggc cacgggaacg atctgcgaga ggaagcgaac
     1441 atcggcgtcg gtgggatcgg cgatccatcc ctctcgctga aagtagggga cggccagccg
     1501 cgccagccgc tcgggcgccg cgctcttcag gtaatgcctg ttcacccacg ccagcttctc
     1561 gacgtcgaac acaccggcgc tgtggccgac gtcctcgaga cggaacagcg cggccagctc
     1621 gccgaccggc agcagttccc ggccgtcccc gggcgaccac cccaggagcg ccaggtaatt
     1681 cgccagcgcc tcaggcagga agccgcgcgc gcggaactcg gcaaccgacg tggcaccgtg
     1741 ccgcttggac aacgggctgt ggtcgggacc catcacgagc gagacgtgcg cgaaggcggg
     1801 gggccgccag ccgaacgcct cgtacagcag cagctgccgg ggcgtgttgg agatgtgatc
     1861 ctcgccgcga atgacgtgcg tgatctccat cagcgcgtcg tcgatgacga cggcgtagtt
     1921 gtaggcgggc gaaccgtcgg agcgcaggag cacggggtcg ccgatcacgt cggtgttgaa
     1981 ggtcactctg ccgcgaacga tgtcgtcgaa gctcacgtcg cgtgcgtcgg gaacgcggaa
     2041 acggatcacc gcctgttcac cactctcgac gcggtggcgc gcctcgtcct tcgtgagcag
     2101 ccggcagcgg cccgagtact tcggcggccg cccagcggca agcgctgctt cacgatccgc
     2161 ctcgagctgc cgggccggac agaagcagtg gtaggcatgg ccgcccgcca gcagttccac
     2221 ggcatgcgcg cgatagatgt gcagccgctc cgactgccgg tacgggccgt gcgtccctcc
     2281 acgctcgacg ccctcgtccc aatcgaggcc catccaccgc aggtcctcga ggatgcctcg
     2341 ctcggactcg ctcgtcgatc gctcgacgtc ggtgtcctcg atgcgaagaa tgagcgtgcc
     2401 tccgctgccg cgggccagca gccagttgaa gagcgccgta cgcgcgttgc cgacgtggag
     2461 ttgcccggtg gggctggggg cgaagcggag tctaatgggg tcgggagtca tttcttgagg
     2521 gcagtcctat tctcggtcag tgcactgtcc tcgagaaatg actcccgacc ccatttaggg
     2581 gcgcgccagt aaggcgacgg cgtggacggc gattgattcg ccggcgccca tcgaatcgac
     2641 gccttcgttg gtcttgccct tgacgccaat ctgcgacgcg tcgcagccga gcgcattcgc
     2701 caggttcgcg cgaatctgat cggcataggg cgagagcttc ggccgctggg cgatcaccac
     2761 gaggtcgacg ttgacgacgg cgtagccggc ggcgcgcacg agggtggcgg cgcgacggag
     2821 catgtccagg ctgttggcgt ccttccaggc tggatcggta tcgggaaaat gacggccgat
     2881 gtcgcccgcc ccggcggcgc cgagcacggc gtcggttacc gcgtggcaca cggcgtcggc
     2941 gtccgagtgc cccaggagtc cggtgtcgaa cggaatggtc acgcctccga gaatcagcag
     3001 gcgccctggc acgagccgat ggaggtcgta cccgttccca atgcgcaggg cggcgtgccg
     3061 cgaggcatcg atctggaggt accgctccgc gagcgcgagg tcgtccggcg tcgtgatctt
     3121 caggttccgg aggtcgccct cgaccacgcg gacctggtgt ccggcccgct cggccagcat
     3181 cgcttcgtcg gtggcgtcgc cgtcgagcgc cagcgcggcc ctgagcacac cgacgcggaa
     3241 ggcctgcggc gtctgcgcga gataaatctc ttcccgcctc aacgtcgcgg cgatgacgcc
     3301 gccctctttc acctgcttga cggtgtcggg gaccggcaca gccgcaatcg ccgcaccgta
     3361 ctcggacgcc gc
//
LOCUS       DBNGSH010000844         2221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3496655, whole genome shotgun sequence.
ACCESSION   DBNGSH010000844 DBNGSH010000000
VERSION     DBNGSH010000844.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3496655"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="AC16CY_19395"
     CDS             <1..704
                     /locus_tag="AC16CY_19395"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR3356024.1"
                     /translation="HRLLTHRAYRTPKWIEYFLTTCGTLALEGGPIFWVATHRIHHQK
                     SDQDGDPHTPREGTWWAHMGWILFGEGLHHDATVLARYVPDLSRDRYHVWLSNWHWLT
                     NVVVGLGLLVFGGIPYVLWGIFFRTTVGLHATWLVNSATHKWGSRRFKTRDDSTNNWW
                     VALLTFGEGWHNNHHAHPTSARHGLAWYELDQNWIGIRGLQMLGLAWDVKLARLKQPI
                     VEDVLVAVPADEAVA"
     gene            754..1647
                     /locus_tag="AC16CY_19400"
     CDS             754..1647
                     /locus_tag="AC16CY_19400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3356025.1"
                     /translation="MTRRQKAIAFFVALCVLLVAAAISLNIGWIIANADRITPLVLGI
                     VLFGLIIAGLIVYTVFLVLEIKRNEEHDSFINAVTHELKTPIASIRLYLETLQAREVS
                     EERRREFYDVMLADAERLHHTVDQVLKAGVVREKQKAVARGPVDMTALAGECVGLAVV
                     RHHLPADAIALHMPDGASLMVNGDAEELRTVVTNLLDNAVKYSGTTVRVTVSVAAPAP
                     DTVWIRVQDRGVGIPKKQLKRVFNRFYRVHSRGLRHIKGTGLGLYIVRSIARAHGGRV
                     FAQSEGEGHGATFTLELPRLV"
     gene            1644..>2221
                     /locus_tag="AC16CY_19405"
     CDS             1644..>2221
                     /locus_tag="AC16CY_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3356026.1"
                     /translation="MSRILVVEDEQHIADGLRFNLEAESHEAIVARDGEQALEMLLTE
                     RRVFDVVILDVMLPGKDGFTVASELRAAGQFVPILMLTARGRAEDVLRGFESGADDYL
                     PKPFELAILLARIKGLLRRRSWNEQDAEPADTPSDTYSFGGRTLDFGAMEIRAGGRSY
                     RLTQMECDLLKYLVKHAGQAISRGTLLEDVWGL"
BASE COUNT          384 a          746 c          710 g          381 t
ORIGIN      
        1 atcaccgcct gctgacgcac cgcgcctaca ggacgccgaa gtggatcgag tacttcctca
       61 ccacgtgtgg aacgctggcg ctcgaaggcg gcccgatctt ctgggtggcg acgcatcgca
      121 tccaccacca gaaatccgat caggacggtg acccgcacac accccgcgaa ggcacctggt
      181 gggcccacat gggctggatc ctctttggcg aggggctcca ccacgacgca accgtgctcg
      241 cacgctacgt ccccgacctc agccgggatc gctaccacgt gtggctctcc aactggcact
      301 ggctcaccaa cgtcgtcgtc ggcctcgggc tcctggtttt tggcggcatt ccgtacgtgc
      361 tgtggggcat cttcttcagg accaccgtcg gccttcacgc cacctggctg gtgaactccg
      421 cgacgcacaa gtggggctcg cgccggttca agacacgcga cgattcgacg aacaactggt
      481 gggtggcact gctgacgttc ggcgaagggt ggcacaacaa ccaccacgcg cacccgacta
      541 gcgcgcggca cggactcgct tggtacgagc tcgatcagaa ctggatcggg atccgcggtc
      601 tgcagatgct cgggctcgcc tgggacgtca agctggccag gctcaagcag ccgatagtcg
      661 aggacgtgct ggtcgcagtg cccgccgacg aggcggtggc ctagaacggc cggccccctg
      721 cagccggcca ccggctgcgt ctacaatcgc tgaatgacgc gtcgccagaa ggcgatcgct
      781 ttcttcgtcg cactctgcgt cctgctggtc gcggcggcaa tttcgctcaa catcggctgg
      841 atcatcgcca acgccgaccg gatcacgcct cttgttctcg gcatcgtcct cttcgggctg
      901 atcattgccg gcctgatcgt ctacaccgtg ttcctcgttc tcgagatcaa gcgaaacgag
      961 gagcacgaca gcttcatcaa cgccgtcacg cacgagctga aaacgcccat cgcctcgatc
     1021 cgcctctacc tggaaacgct ccaggcgcgt gaagtgagcg aggagcggcg gcgcgagttc
     1081 tatgacgtga tgctggccga cgccgaacgc ctgcatcata cggtcgatca ggtgctcaag
     1141 gcaggcgtgg tccgcgagaa gcagaaagcc gtcgctcgcg gaccggtgga catgaccgcg
     1201 ctcgccggcg agtgcgtcgg cctggcagtc gtgcgtcacc acctgcccgc cgacgcgatc
     1261 gcccttcaca tgcccgatgg cgcttccctc atggtcaacg gcgatgccga ggagctcagg
     1321 acagtcgtca ccaacctgct cgacaacgcc gtgaagtact cggggacgac cgtgcgcgta
     1381 actgtgtcgg tggcggcgcc ggcgcccgat accgtgtgga tccgcgtcca ggatcgcggc
     1441 gtcgggatcc cgaagaaaca gctcaagcgg gtcttcaacc gtttctaccg cgtccactcg
     1501 cgcggcctcc ggcacatcaa gggtaccggt ctcgggctct acatcgtccg atcgatcgct
     1561 cgcgcgcacg gcgggcgagt gttcgcgcag agcgagggcg agggccacgg cgccaccttc
     1621 acgctcgagc tgccccggct ggtatgagcc gcattctcgt cgtcgaggac gagcagcaca
     1681 tcgccgacgg cttgcgcttc aatctggagg cggagagcca cgaggcgatc gtcgcgcgag
     1741 acggcgagca ggcgctcgag atgctcctca ccgaacgccg tgtcttcgac gtcgtgatcc
     1801 tggacgtgat gctgcctggc aaggacggct ttacggtcgc ctcggagctg cgcgccgctg
     1861 gacagttcgt cccgatcctg atgttgacgg cccgcggacg ggcggaggac gtgctgcgcg
     1921 gtttcgagtc cggcgccgac gactacctgc cgaagccctt cgagctcgcg attctcctgg
     1981 cccgcatcaa ggggctgttg aggcggcgat cgtggaacga gcaggatgcc gaaccggccg
     2041 acacaccgtc cgacacgtat tcgttcggcg ggcgcacgct cgatttcggc gcaatggaga
     2101 tccgcgccgg cggccgatcc tacaggctga cccagatgga atgcgatctg ctcaagtacc
     2161 tggtcaagca cgccggacag gcgatttcgc gcggcacgct gctcgaggac gtctggggtc
     2221 t
//
LOCUS       DBNGSH010000845         4105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2308925, whole genome shotgun sequence.
ACCESSION   DBNGSH010000845 DBNGSH010000000
VERSION     DBNGSH010000845.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2308925"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="AC16CY_19410"
     CDS             complement(<1..830)
                     /locus_tag="AC16CY_19410"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3356027.1"
                     /translation="MTRRFTPFLLAFMLLAGFIAGSMLSGPIREQTAAGAQTSGQSRQ
                     VINTPGPTSAAATLTDFSRIAERTVPAVVNISAQQLVRRQYVDPFDFFSRPDVFSSRG
                     VQNSLGSGVIVSPDGFVLTNYHVVTGENRRVTIEQVDVTVTLADKRELRADLVGYDSA
                     TDLALLKIGATGLPKIPWGDSSRLKVAEWVLAIGNPYQLSETVTLGIVSAVGRTNLGV
                     SRYEDFIQTDAAINPGNSGGALVNTRGELVGINTFIYSESGGYQGIGFAVSGNLAQRI
                     V"
     gene            complement(845..1246)
                     /locus_tag="AC16CY_19415"
     CDS             complement(845..1246)
                     /locus_tag="AC16CY_19415"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3356028.1"
                     /translation="MAFVRWDPLRDLLAIQQRLNRLSPGPAGWTPPVDLLETPDAYIL
                     TMELSGIEQDEVQLQLRDGRLTIAGVRRERESAFEQYHQIERGHGRFSRSFDLPAAVE
                     TERVTADLRDGVLTVTCPKSSEGPSRRIDVR"
     gene            1364..1927
                     /gene="efp"
                     /locus_tag="AC16CY_19420"
     CDS             1364..1927
                     /gene="efp"
                     /locus_tag="AC16CY_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014215531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3356029.1"
                     /translation="MATLQATRLKKGMLVKMDQDLYRVLDLQHVTPGNLRGFVRIKMR
                     NIRNGTLSDQKLRSEDSVERATLDERQMQYLYQDGTDYYFMDTTTFEQVHISSEALGD
                     SVNYLKAEMTIQVEFYGSEPVGIELPVTVDLKVTDTAPGIKGATASAQVKPATLETGL
                     VVQVPPFVNPGDSVRVSTDTGEYLSRA"
     gene            2019..2594
                     /locus_tag="AC16CY_19425"
     CDS             2019..2594
                     /locus_tag="AC16CY_19425"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR3356030.1"
                     /translation="MMDVVRQPVQGWIEVIVGSMFSGKSEELIRRLRRARIARQKVQV
                     FKPRIDTRYSTDHIVSHGEMRMESCAVDSSRQLLDMVEGDTEVVGIDEGQFFDPDLPA
                     ICETLADRGLRVIVAGLDQDYLGKPFEPMPQLLAVAEFITKTHAICMVCGNPANHTQR
                     LVASADRVLVGADGLYEARCRRCFDPALSRV"
     gene            2683..3267
                     /locus_tag="AC16CY_19430"
     CDS             2683..3267
                     /locus_tag="AC16CY_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356031.1"
                     /translation="MTTAEWIPWFAWLAPRLLLLFGVGFLVANLRLGASLWAYRQRSR
                     SAALVWEAARPRYYRFSLILGGILFVLLVVEAVVLRRSPWSIFGEAMMVVYYGFAFPL
                     STRIRRGFYGDGVWADRAFVKWSEISAVSWKEGAALTLMLISHARNVARPLSVPGHLY
                     GEARRLLRDRIKAHDIRIGGAGLELGAREDRDAV"
     gene            3309..>4105
                     /gene="ku"
                     /locus_tag="AC16CY_19435"
     CDS             3309..>4105
                     /gene="ku"
                     /locus_tag="AC16CY_19435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end joining protein Ku"
                     /protein_id="HIR3356032.1"
                     /translation="MPARPTWKGYLKVSLVTIPVKVFPATESAAALSFNQLHAECQTR
                     IQQKRWCPHCEREVSNAELAKGYEFEKGRYVVVSDEDIQNVRVESTRVIDLAQFTEDS
                     AIDPIYVDRAYYLAPDGAMAASAFAVMREGMAGKAGIGKVALYGREYLVAVRPQKKGL
                     VMYTLHHDAEIRSIDQIEELGSLPSKVKPEEMKLAKQVISSFEGELNLKDYKDEYKEG
                     LRKIIDAKIAGEEIVAPEVQEPPKVVDLMEALRKSLDSVSADKKKLA"
BASE COUNT          731 a         1268 c         1403 g          703 t
ORIGIN      
        1 acgatccgct gcgccagatt gccggacacc gcgaagccga tcccctggta gccgccgctc
       61 tcgctgtaga tgaaggtgtt gattccgacc agttcgcccc gcgtgttcac gagcgccccg
      121 cccgagttgc cggggttgat cgccgcatcg gtctgaatga agtcctcgta tctcgagacg
      181 ccgaggttcg tgcgaccgac ggccgagacg atgccgagcg tgactgtctc gctgagttga
      241 taggggttgc cgatggcaag gacccactcg gccactttca gacgggaaga atcgccccac
      301 gggatcttcg gaaggccggt cgccccgatc ttcagcagcg cgagatcggt ggccgagtcg
      361 tagcccacga gatcggcgcg cagctcgcgc ttgtcggcca acgtgacggt gacgtccacc
      421 tgctcgatcg tgacgcggcg gttctctccg gtcacgacgt ggtagttggt gaggacgaat
      481 ccgtccgggc tgacgatcac gccggaccca aggctgttct gcacgccgcg tgagctgaat
      541 acgtccgggc ggctgaagaa gtcgaaggga tcgacgtact ggcggcgcac gagctgctgc
      601 gccgagatgt tgacgacggc cggcaccgtc cgttcggcga tgcggctgaa gtcggtgagc
      661 gtcgccgccg ccgacgtggg ccccggcgtg ttgatcacct ggcggctctg gcccgacgtc
      721 tgagcgccag ccgccgtctg ctcgcgaatg gggcccgaga gcatcgagcc ggcgatgaac
      781 cccgccagca gcatgaacgc gagcaggaac ggcgtgaagc ggcgcgtcat ggtccttggg
      841 ttcgtcacct gacgtcgatg cgccgcgagg gtccctcgga ggacttcgga catgtcaccg
      901 tgagcacgcc gtcgcgcagg tcggcggtga cgcgttccgt ctcgacggcc gcgggcaggt
      961 cgaacgagcg gctgaagcgg ccgtggccgc gctcgatctg gtgatactgc tcgaaggccg
     1021 actcgcgctc gcggcggacg ccggcgatcg tcagtcggcc gtcgcggagc tggagctgca
     1081 cctcatcctg ctcgatgccc gacagctcca tggtcaggat gtaggcgtcc ggcgtctcga
     1141 gcagatcgac cggaggcgtc cagcccgccg gccccgggga gagccggttg agtcgttgct
     1201 ggatggcgag cagatctcgg agcggatccc agcgtacgaa cgccacgcct gaatcgtacc
     1261 acaagggtgg atggtggttc gtggatgatg gctggcggcg atggcaggcc caaggttggc
     1321 agcggccttg tgctacgatc gacaggttct gcagactgca tttatggcca ccttacaggc
     1381 aacgcgactc aagaagggca tgctcgtcaa gatggatcag gatctctacc gggtcctcga
     1441 cctccagcat gtgacgccgg gcaacctccg cggtttcgtc cgcatcaaga tgcggaacat
     1501 ccgcaacggc acgctgtccg atcagaagct tcgatcggag gattccgtcg aacgggcgac
     1561 cctggacgag cggcagatgc agtacctcta ccaggacggc accgactact acttcatgga
     1621 caccaccacg ttcgagcagg tccacatctc gagcgaggcg ctcggcgact cggtgaacta
     1681 cctgaaagcc gagatgacca tccaggtcga gttctacggc agcgaaccgg tcggcatcga
     1741 gcttccggtg acggtggacc tgaaggtgac cgataccgcg ccgggcatca agggcgccac
     1801 ggcaagcgcg caggtcaagc cggcgacgct cgagacgggc ctcgtcgtgc aggtcccgcc
     1861 gttcgtgaac ccgggcgact cggtccgggt cagcaccgac acgggggaat atctgtcaag
     1921 agcttagtca actcccaatc tccaactccc aattcccaag tacgaaggac aacgaactct
     1981 tttgggtcgg gagttgggag ttgggagttg ggagttgaat gatggatgtg gtgcgccagc
     2041 ccgtccaggg ctggatagaa gtcatcgtcg gcagcatgtt cagcggcaag agcgaggagc
     2101 tgattcgccg cctgcggcgc gcgcggatcg cgcggcagaa ggtgcaggtc ttcaagcccc
     2161 ggatcgacac gcgctattcg accgatcaca tcgtgtcgca cggcgagatg cggatggaat
     2221 cgtgcgccgt ggacagctcg cggcagctcc tcgacatggt cgagggagac accgaggtcg
     2281 tcgggatcga cgaggggcag ttcttcgacc cggatcttcc ggcgatctgt gaaacgctcg
     2341 ccgatcgtgg attgcgcgtg atcgtggccg gcctcgatca ggactatctc gggaaaccgt
     2401 tcgagccgat gccgcagctg ctcgccgtgg cggagttcat caccaagacc cacgcgatct
     2461 gcatggtgtg cggcaacccc gcgaaccata cccagcgact ggtcgcgagc gcggatcgcg
     2521 tcctcgtggg cgccgacggc ctgtacgagg cacgatgccg ccggtgcttc gatccagcgc
     2581 tctcccgcgt gtaaactccc aactcccagt ctccaactcc caatcgcggt cacgtctgcg
     2641 ataacgttgg gaattgggcg ttgggagttg ggggttgaag acgtgacgac cgcggagtgg
     2701 atcccctggt tcgcgtggct ggcgccgcgg ctgctcctgc tgttcggtgt gggctttctg
     2761 gtcgcgaacc tcaggctcgg cgcctcgctg tgggcctacc ggcagcgaag ccggtccgcg
     2821 gcgctcgtct gggaagcggc caggccgcgg tactaccggt tcagcctcat cctcggcgga
     2881 atcctcttcg ttctgctggt cgtggaggcg gtcgtcctcc ggcgatcgcc atggtcgatc
     2941 tttggcgagg ccatgatggt cgtctactac ggcttcgcgt tccccttgag cacgcgtatc
     3001 cgcagggggt tctacggcga cggcgtctgg gccgaccggg cgttcgtgaa gtggtcggag
     3061 atctcggccg tgtcgtggaa ggagggagcg gcgctcacgc tcatgctgat ttcgcacgcc
     3121 cggaacgtgg cgcgccccct gtcggtcccg gggcatctct acggcgaggc ccgccgcctc
     3181 ctgcgtgacc ggatcaaggc gcacgacatc cgtatcggcg gcgccgggct cgagctgggg
     3241 gccagggagg atcgcgacgc agtatgaatt gaagccctga acccattgca ccgataatgt
     3301 tcggacatat gcctgcacgt cctacgtgga aggggtacct gaaggtcagc ctggtcacca
     3361 tccccgtgaa ggtgttcccg gccaccgagt ccgccgcggc gttgtcgttc aaccagctcc
     3421 acgccgagtg ccagacacgc atccagcaga agcgctggtg tccgcactgc gagcgggagg
     3481 tgtccaacgc ggagctcgcc aaggggtacg agttcgagaa agggcgctac gtcgtggtgt
     3541 ccgatgagga catccagaac gtccgcgtcg aatcgacccg cgtgatcgac ctcgcgcagt
     3601 tcacggaaga ctcggcgatc gatccgatct acgtggaccg ggcctactac ctcgcgccgg
     3661 acggcgcgat ggcggcatcc gcctttgccg tcatgcgcga ggggatggcc gggaaggcgg
     3721 gcatcgggaa ggtggcgctc tacgggcggg agtacctcgt cgccgtccgg ccccagaaga
     3781 aggggctcgt gatgtacacg ctgcaccacg acgccgagat ccgcagcatc gaccagatcg
     3841 aggagctggg atcgcttccg tcgaaggtca agcccgaaga gatgaagctc gccaagcagg
     3901 tgatctcctc gttcgagggc gagttgaacc tcaaggacta caaggacgag tacaaggaag
     3961 gtctgcggaa gatcatcgac gccaagatcg ccggcgagga gatcgtcgcg cccgaggtgc
     4021 aggagccgcc gaaggtcgtc gacctgatgg aggcgctgcg caagagcctc gactcggtga
     4081 gcgccgacaa gaagaagctg gcgaa
//
LOCUS       DBNGSH010000846         3396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_780982, whole genome shotgun sequence.
ACCESSION   DBNGSH010000846 DBNGSH010000000
VERSION     DBNGSH010000846.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_780982"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..3001
                     /locus_tag="AC16CY_19440"
     CDS             80..3001
                     /locus_tag="AC16CY_19440"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3356033.1"
                     /translation="MGEGPARNVNHRNHVGPQQRVLSETASALAESDTLEAAAPRMLE
                     AVCRALDWQFGAIWEVDRAKNVLRCAGTWHQPAPQLEEFSKATREATFPAGVGLPGRV
                     WLHPEPAWIPDVTRDPNFPRAPFAERAGLHAAFGLPILQGSSVVGVMEFFSSEIFEPT
                     ADLKSLMTMICGQIGLYVERKWTAEELDRFFKLSLDLLCVATFDGYFVRANPAWQTLL
                     GYTEDELRVSPFMDFVHPDDKAATIQAMSALLTGEHVLDFENRYRSKGGSYRWLQWRS
                     APFAKQGLIYAAARDVTERKLAEEERERHAQDLQRLVKELEVSRQRAEQATVAKGEFL
                     ANMSHEIRTPMNAIIGMTDLALQTKLTAQQRDYLRAAKESAEALLTIINDILDVSKIE
                     ARKLTLDRSPFRFRDAVEDSVRLLAPRADQKGLELACRIAPDVPDALVGDAGRLRQVI
                     LNLVNNAIKFTDEGEVVVDVSLDDRTDETATLRFLVRDTGIGIAPEKQWEIFGAFVQA
                     DASTTRRYGGTGLGLTISAQLVEMMDGRLWVESDVGKGTKFHFVAQFGIHKGATEAAH
                     STAGSLRDLRALVVDDNETNRLILGEILASWQMQASAVGGAAAALAALYDAADRGQPF
                     HLVLTDALMPDVDGFTLAQQIARDGRLSGVKMILLTSAGSSSGRGRPTGHFAAQLAKP
                     VKQSDLLDAIVTAFAAPGGPEPPRERRVRGPRRKVTRSLRVLVAEDNPTNQKLVVALL
                     KQQGHRVSMVGNGRLAVERAAKQRFDVILMDVQMPEMGGFEATAAIRERERATGTHTP
                     IVALTAHAMAGDRERCLEAGMDGYISKPLRPDELFSTIDLMAPSGGAGPTQERGLSNA
                     GRVDLTTLLANFGGRASVVKGVIDVFLEDAPNMLARLKQATDAKDAGELASAAHAIKG
                     SAGLFLQGYAYESARRLEHYAKAGDLSELDAAYADVEGGVSRLMVELRSIRKALGEKK
                     D"
     gene            complement(3175..>3396)
                     /locus_tag="AC16CY_19445"
     CDS             complement(3175..>3396)
                     /locus_tag="AC16CY_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3356034.1"
                     /translation="VEVQLRDGRVVSHFTRHAPGTKENPLDTAGVNAKARSLMAPVLG
                     ARRCETVIERVNGLEQLSSVRDLAVLLGA"
BASE COUNT          583 a         1110 c         1187 g          516 t
ORIGIN      
        1 gcttgcctcc gggagtgtcc tcaagaaatg actcccgacc ccattttctc ccgaccccgt
       61 gacagcgtag tgtaatgcca tgggcgaggg tccggcgcgc aacgtgaatc accgtaacca
      121 tgtcggcccg cagcagcggg tgctgtcgga aacggcgagc gcactggccg aatccgacac
      181 gctggaggcg gccgctcccc ggatgctcga ggccgtgtgc cgggcgctcg actggcagtt
      241 cggcgccatc tgggaggtag accgcgccaa gaacgtcctg cgctgcgccg gcacgtggca
      301 tcagccggcc ccgcagctcg aagagttctc gaaggccacg cgggaggcca cctttccggc
      361 gggcgtcggc cttcccggac gcgtctggtt gcaccccgag cccgcctgga ttcccgacgt
      421 cacccgcgat cccaactttc cccgcgcgcc attcgccgag cgcgcgggcc tgcacgcggc
      481 gttcggcctg ccgatccttc aaggctcgag cgtcgtcggc gtcatggagt tcttcagcag
      541 cgagatcttc gagccgactg ccgatctgaa gtcgttgatg accatgatct gcggtcagat
      601 cgggctctac gtcgagcgga agtggacggc ggaggaactc gaccggttct tcaagctgtc
      661 attggatctg ctctgcgtgg ccaccttcga cgggtacttc gtgcgggcga acccggcgtg
      721 gcaaaccctg cttggttaca cggaggacga gctgcgcgtg tcgcccttca tggacttcgt
      781 gcaccccgac gacaaggcgg ccacgatcca ggcgatgtcg gcgctgctga ccggcgagca
      841 cgtgctggac ttcgagaacc ggtaccgatc gaagggcgga tcgtaccggt ggctgcagtg
      901 gaggtccgcg ccgttcgcca agcaggggct gatctacgcg gccgcccgcg acgtgaccga
      961 gcgcaagctg gcggaagagg agcgggagcg gcatgcacag gacctgcagc gcctcgtgaa
     1021 ggagctggag gtctcgcgcc agcgcgccga gcaggccacc gtggcgaagg gcgagtttct
     1081 cgccaacatg agccacgaga tccggacgcc gatgaacgcc atcatcggca tgaccgacct
     1141 cgcgctgcag acgaagctga cggcccagca gcgggattac ctgcgggcgg ccaaggagtc
     1201 cgccgaggcg ctcctgacga tcatcaacga catcctggac gtgtcgaaga tcgaagcacg
     1261 gaagctgacg ctcgaccggt ctccgttccg cttccgcgac gcggttgagg acagcgtgag
     1321 gctgctcgcg ccgcgcgccg atcagaaggg attggagctg gcgtgccgca tcgcgcccga
     1381 cgtgccggac gcgctcgtcg gcgacgccgg ccgccttcgt caggtcatcc tcaacctggt
     1441 gaacaacgcg atcaagttca ccgacgaggg cgaggtcgtc gtggacgtgt cgctggacga
     1501 ccggacggac gagaccgcga cgctgcggtt cctggtcagg gacaccggca tcggcatcgc
     1561 accggaaaag cagtgggaga tcttcggcgc gttcgtgcag gccgacgcgt ccacgacgcg
     1621 gaggtacggc gggacgggcc tgggcctgac gatctccgcg cagctcgtcg agatgatgga
     1681 cggacgcctg tgggtggaga gcgacgtcgg caaggggacg aagtttcact tcgtcgcgca
     1741 gttcgggatc cacaagggcg cgaccgaggc agcccactcg accgccggga gcctgcgtga
     1801 cctgcgcgcg ctcgtcgtgg acgacaacga aacgaatcgg ctgatcctcg gcgaaatcct
     1861 cgccagctgg cagatgcagg cctcggccgt cggcggcgcg gcggcggcgt tggcggcgct
     1921 gtacgacgcg gcggaccggg ggcagccctt tcacctcgta ctgaccgacg cgctgatgcc
     1981 cgacgtggac gggttcacgc tcgcgcagca gatcgcgcgc gacggcaggc taagcggggt
     2041 caagatgatc ctcctgacgt cggcgggatc ctcgtcgggg cgcggacgac cgacgggaca
     2101 tttcgccgcg caactggcga agccggtcaa gcagtccgat ctgctcgacg cgatcgtcac
     2161 ggccttcgcg gctccgggcg gacccgagcc tccgcgcgag cgccgggtgc gcgggccgcg
     2221 ccggaaagtg acgcgctcgc tgcgcgtgct cgtggcggaa gacaacccca ccaaccagaa
     2281 gctggtcgtg gcgctgctga agcagcaggg gcaccgcgtg tcgatggtcg gcaacggccg
     2341 cctggccgtc gagcgggccg cgaagcagcg cttcgacgtg atcctcatgg acgtgcagat
     2401 gcccgagatg ggaggcttcg aggccactgc ggcaatccgg gagcgcgagc gcgcgacggg
     2461 cacgcacacg ccgatcgtgg cgctgaccgc gcacgccatg gcgggcgacc gcgagcgctg
     2521 cctggaggcg ggcatggacg gctacatctc caagcctctc agaccggacg agctgttctc
     2581 gacgatcgac ttgatggccc cttccggcgg cgccggcccg acacaggagc gcggactcag
     2641 caatgccggg cgcgtggacc tcaccactct gctggccaac ttcggcggac gcgcgtccgt
     2701 cgtcaagggc gtgatcgacg tcttcctcga ggacgcgccg aacatgctgg ctcggctgaa
     2761 acaagcgacc gatgcgaagg acgccggtga actggcctcg gccgcccacg caatcaaggg
     2821 ctcggccggg ttgttccttc aaggctatgc gtacgagagc gcgcgccggc tggaacacta
     2881 cgccaaggcc ggggatctca gcgagctcga cgccgcttac gcggacgtcg aaggaggcgt
     2941 ctcgcgattg atggtcgagt tgcgctcaat caggaaagcc ctcggggaga agaaggacta
     3001 gctatctttg gtccttggtc cttggggctt cgttcttggt ccgtccttcg ttcttggtcc
     3061 atggtccggc gtctacagga ccttggacct tggaccaagg acggaccaag gaccaaggac
     3121 ggaccaagga ccagggacgg accaaaggac caggcaccaa ggaccaagga ccaattacgc
     3181 gccgagcaag acggcgaggt cgcgcacgct cgagagctgc tcgaggccgt tgacgcgctc
     3241 gatgacggtc tcgcatcgcc gggcgccgag cacgggagcc atgaggctcc gtgccttggc
     3301 gttcacgccc gccgtgtcga gcgggttctc cttcgttccg ggcgcgtgtc gcgtgaagtg
     3361 gctgaccacg cggccgtcgc gaagctgcac ctccac
//
LOCUS       DBNGSH010000847         7316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3158420, whole genome shotgun sequence.
ACCESSION   DBNGSH010000847 DBNGSH010000000
VERSION     DBNGSH010000847.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3158420"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="AC16CY_19450"
     CDS             complement(<1..275)
                     /locus_tag="AC16CY_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380453.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR3356035.1"
                     /translation="MTETATKETLGFQAEVKQLLHLMVHSLYGNKEIFLRELVSNASD
                     AGDKLRFEAMTDGALFENDPDPKIRVSYDAGARTITVSDNGIGMSRQ"
     gene            complement(344..1414)
                     /locus_tag="AC16CY_19455"
     CDS             complement(344..1414)
                     /locus_tag="AC16CY_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356036.1"
                     /translation="MSRPRRRVCPESGGCRGSAGTAGHDAARGHRAVSLPIPGPAPGF
                     VQPKTPWGDPDLQGFWPGVDMVGVPLQRPARFGTRNVLTEEEFSQRARDIKAEEESLV
                     AEIDVFTADVTTPCAIRCPTSPQPHWQETGKPSRQASLIIDPPDGRQPPLSQEGQARQ
                     ARQQADNKARLARLQGREADSHLDRSLYDRCITRGLLGSLLPTIYNNGNEIVQGPGYV
                     VIRNEMIHEARVVPLDGRPHLPAGVTSYMGDSRGRWEGNTLVVVTKNLNGMTGAGGNG
                     GGRTSGRITITERFTMLDRNTLQYTATIDDPGTWTRPWTLSFPWKREPVYGMFEYACH
                     EGNYAMRNMLSISRAAEAGVGR"
     gene            1528..2484
                     /locus_tag="AC16CY_19460"
     CDS             1528..2484
                     /locus_tag="AC16CY_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356037.1"
                     /translation="MRPARAFVRGVLTAALIAGVYHPALADQQPSGWTHPRTPWGDPD
                     LTGRWPITDLNGTPVQRPANLGERRLLTDDEYAQRVKQIESTRERTAGAWAEIGQPNR
                     LTSLVVEPASGRLPPLTAEGRRRAAAMTSSWSDMLFDRVDQFNALDRCITRGLPASMF
                     PFMYNSGMEIVQAPGVVVLRLELIHETRIIPVDGRAPLSPALRHWMGESRGRWEGTTL
                     VVETTNFNGRSPMMIVGPGGNSIPTSESLRIVERFTRATSDRLDYELSVEDPDVLTGP
                     WKAAFPWTRDPSYYIFEYGCHEGNQAIVNALRNSRYLETTKP"
     gene            complement(2527..3813)
                     /locus_tag="AC16CY_19465"
     CDS             complement(2527..3813)
                     /locus_tag="AC16CY_19465"
                     /inference="COORDINATES: protein motif:HMM:NF028479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HIR3356038.1"
                     /translation="MERLVKIAAACIALSVVILLSNRMGAVQGQQKRTPGQGFAAVPG
                     LKGGNDAFGPYDPVANWPRPMGESLPDHQAWTWSQSTDIFPESPDRVFVTQKGELPVL
                     QLGRGTTWLPQIGPSIKFPVGGGLPVRETASATPSFGRAAANGSNGRPGVDWRWEHVI
                     VVFDRNGRMIDTENWAQWDKIWGRPHDVEISPYDPEKNVWIVDADAHFVSKFSHDGKQ
                     RLLTLGTPNMPGTDDTHFSRPTFMAFMDKNTWYLADGYDGTRVIKYDMNGKKLLEWGM
                     RGTPPNEKRPGYMNNVHGIAVNPKTRRVYVNDRNNGRLQVFDENGKFLDQWDFGPRPP
                     MNIHSIYMGSDGILWAADQGSNKLLAYDADGHFLYSWGTFGTCQGCQWGVHGFATDKE
                     GNLYTAEVRTGRVQKYTPRKGANPEFLVGKPWPGVW"
     gene            complement(3892..4848)
                     /locus_tag="AC16CY_19470"
     CDS             complement(3892..4848)
                     /locus_tag="AC16CY_19470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3356039.1"
                     /translation="MNSIRSTSAFAFAALLAATAVSAQQPPAPPAAAAPATTFEVASV
                     KPSPPGDPSNPLSMMPMAAPRPGGRFTATNMPLWALISTAWELPDFRIVGGDKELMNT
                     KYHITAKTEGGATLGMKAALPMLKNLITERFQLKFHFEPREMSHYDLVLARSDGQTGP
                     ELKPSKSDCSNADELNATRAEGQAKGDFAALIPKPGEFFACTIAPNLAGGPANLSLHG
                     DGQEIKVLVDLLSQFTGKHVRDKTGLTGLYDFDMKLDMQALLGLVQKLGVDVPTAALS
                     NLQSDGSSVMTALREQLGLKLESTRGPVDVLVIDSVEAPTQD"
     gene            complement(4947..5183)
                     /locus_tag="AC16CY_19475"
     CDS             complement(4947..5183)
                     /locus_tag="AC16CY_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356040.1"
                     /translation="MLNRWTGVGLLIAGTAIGFGAAGLRESPVASAQAVQSKTTELKE
                     SIEVDAPWLGAAAHLEITATGVSIAGRYKVACEQ"
     gene            complement(5202..7034)
                     /locus_tag="AC16CY_19480"
     CDS             complement(5202..7034)
                     /locus_tag="AC16CY_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356041.1"
                     /translation="MDLPGPRRPQPPAGGGGMTMRSPIIAMLWENWRLTRAEAAWHLA
                     LGIVAASAVLVALAAVAAVAGNEAAKDRWAVMALIVVFVPHVIGWFSVNKLMVERAGF
                     PFHLLYTRPVRTAVAVGIPMAYLAALPAASYLVSALLLRITSGHAFPLLPAAAWITAL
                     NLTSWAVNWSIRNVAVRTLAHLAASVAWSAAPIYLLNEEIPGPGFAPLHLWPTVFDLP
                     LTCYALIGAIGLASYGLTVAAVARQRRGDGRAAISWIPGSGFPDRLVSLFRFPCPISS
                     ATRAQVWFELRSRGLPLLAIGAALAIVNPLLFAVSGPIDAALYDGVRQYVPCADSGCF
                     YARALAMLFAILSVVTVLGLGGNAFGIRWRPGHRYASAFEATQAYGTARLAGLKVLVR
                     SVCQLAAIFAVGVSVWASQSFIAVDKGYAPLVEIADEPLENWQRAIEGAVGALTGYEK
                     LALAAVAFTGVAIWVASWAALGALATRYPRRVNTASSLLLLHGLALVLLALAGQRGIG
                     PEFLLPAMLRATKWLAVAAIVLATVYLVSSSFAERLLTLGQASGAVLVSAAFGATWVI
                     VLRAAGVQLSGMPAMDVVWMLSPALLPLMTSVLAPWSFSRARHT"
     gene            complement(7067..>7316)
                     /locus_tag="AC16CY_19485"
     CDS             complement(7067..>7316)
                     /locus_tag="AC16CY_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356042.1"
                     /translation="LPNVTLRVDAESGDLLALTTTLVMPGLGPVPSTTRFEDYRLVDG
                     VRLPFRQVESNEQLGRTVYEIEHVELDIEIENEQFVLR"
BASE COUNT         1240 a         2449 c         2366 g         1261 t
ORIGIN      
        1 tcctgccgcg acatgccgat gccgttgtcc gacacggtga tcgtccgcgc gccggcgtcg
       61 tagctgacgc ggatcttcgg gtccgggtcg ttctcgaaca gcgccccgtc ggtcatcgcc
      121 tcgaagcgca gcttgtcgcc cgcatcggac gcgttcgaga ccagctcgcg caggaagatc
      181 tccttgttgc cgtacaagga gtggaccatc aggtggagca gttgcttcac ctcggcctgg
      241 aagccgagcg tctctttggt ggccgtttcg gtcatgtcga atccaacgat ctccagcgcg
      301 atccgacgcg gctggcgccg cagcgggaag gggagtgcgg gggctacctg cccacgcccg
      361 cctcggccgc gcgcgaaatg ctcagcatgt tgcgcatcgc gtagttgccc tcgtggcacg
      421 cgtactcgaa catgccgtag accggctcgc gcttccacgg aaacgacaag gtccacggcc
      481 gcgtccacgt ccccgggtcg tcaatggtcg cggtgtattg gagcgtgttc cgatcgagca
      541 tcgtgaaccg ctcggtgatc gtgatccggc cgctcgtccg gccgccgccg ttcccgccgg
      601 cgccggtcat gccgttcagg ttcttcgtca cgacgaccag cgtgttcccc tcccagcggc
      661 cgcgcgagtc gcccatgtag gacgtcacgc ctgccggcag atggggacgg ccgtccagcg
      721 gcaccacgcg cgcctcgtgg atcatctcgt tgcggatcac cacgtacccc ggcccctgta
      781 cgatctcgtt cccgttgttg tagatggtcg gcaggagcga accgaggagg ccgcgcgtga
      841 tgcagcgatc gtagaggctg cgatcgagat ggctgtccgc ctcgcgcccc tggagccgcg
      901 cgagccgcgc cttgttgtcg gcctgctgcc gcgcctgacg cgcctgtcct tcctgcgaca
      961 acggcggctg ccggccgtcc ggcgggtcga tgatgagcga cgcctgccgc gacggctttc
     1021 ccgtctcctg ccagtgcggc tgcggcgacg tcggacagcg gatggcacat ggcgtcgtca
     1081 cgtcggcggt gaacacgtcg atctcggcga caagcgactc ctcctcggcc ttgatgtcgc
     1141 gggcgcgctg agagaactct tcctcggtga gcacgttgcg cgtgccgaac cgcgcgggcc
     1201 gctgcagcgg cacgcccacc atgtcgacgc ccggccagaa cccctgcaga tcggggtcac
     1261 cccatggggt cttcggctgg acgaatcccg gcgcgggtcc gggaatcggc aggctgacgg
     1321 cccggtggcc tctggcggcg tcgtggccgg cggtgcctgc tgagccgcgg cagccaccgg
     1381 actcagggca aacgcggcga cgagggcgag acatgaggtt ttcatggcgt gctccagcaa
     1441 agacagtatg gtaacgcgcc cacgccctgc gctgaacagg gtgttcgcgg caagacgagc
     1501 gacgaccggc gcgtataatc tgcgcgcatg aggcccgcgc gcgcgttcgt gaggggcgtt
     1561 ctgacggccg cgctcatagc cggcgtctat cacccggcac tcgcggacca gcagccatcg
     1621 ggctggacac atccgcggac gccgtggggc gatcccgatc tcaccggcag atggcccatc
     1681 acggatctga acggcacacc ggtgcagcgt cccgccaacc ttggcgagcg gcgattgctg
     1741 accgacgacg agtacgcaca gcgcgtgaag cagatcgaat cgacccgtga gcgcacggca
     1801 ggcgcatggg ccgagatcgg tcagccgaac cggctgacgt cgctcgtcgt cgaacccgcc
     1861 agcggcaggc tgccgccgct cacggcggaa ggccggaggc gcgcggcggc gatgaccagc
     1921 tcgtggtcgg acatgctgtt cgatcgcgtc gatcagttca acgcgctcga ccgctgtatc
     1981 acgcgaggtc ttccggcctc gatgttccct ttcatgtaca acagcggcat ggagatcgtt
     2041 caggcgcccg gcgtcgtggt gctccgcctc gagctgatcc acgaaacccg gatcatcccc
     2101 gttgacggcc gggcgcctct gtcaccggcg ctcaggcact ggatgggcga gtcgcgcggc
     2161 cgctgggagg gcacgacgct ggtcgttgag acgacgaact tcaacggccg gtcgccgatg
     2221 atgatcgtcg gacccggcgg caactcgatc ccgacgagcg aatcgttgcg gatcgtcgag
     2281 cgcttcaccc gtgcgaccag cgatcggctc gactacgagt tgagcgtgga ggatcccgac
     2341 gtcctgaccg ggccgtggaa ggccgcgttt ccgtggacgc gggatccgag ctactacatc
     2401 ttcgagtacg gctgccacga gggaaaccag gcgatcgtca acgcgctgcg aaactcgcgg
     2461 tatctcgaga cgaccaagcc gtaaaacagt caccgctcca ggctcgtgca gcccgctccg
     2521 aggctgctac cacactcccg gccagggttt gcccacgagg aactccggat tcgcgccctt
     2581 gcgcggcgtg tacttctgga ctcggccggt tctcacttcc gccgtgtaga gattgccttc
     2641 tttgtcggtc gcgaacccgt gcacgcccca ctggcagccc tggcacgtac cgaacgttcc
     2701 ccatgaatag aggaagtgtc cgtcggcgtc gtaagcgagg agcttgttcg acccctggtc
     2761 ggcggcccac aggatgccgt cgctgcccat gtagatgctg tggatgttca tcggcgggcg
     2821 cgggccaaaa tcccattgat cgaggaactt tccgttttca tcgaacacct ggaggcgtcc
     2881 gttgttgcgg tcgttcacgt acacacgccg cgttttcggg ttgacggcga ttccgtggac
     2941 gttgttcatg tagccggggc gcttctcgtt gggaggcgtg cccctcatgc cccactccag
     3001 aagcttcttg ccgttcatgt cgtacttgat gacgcgtgtg ccgtcatagc cgtcggcgag
     3061 ataccaggtg ttcttgtcca tgaaggccat gaaggtcggc ctgctgaaat gcgtatcgtc
     3121 cgtgcccggc atgttcggcg tgcccagtgt caggaggcgc tgtttgccgt cgtgcgagaa
     3181 cttgctgacg aagtgggcgt cggcatcgac gatccagacg ttcttctccg gatcgtaggg
     3241 gctgatctcg acgtcgtgtg gccggcccca gatcttgtcc cactgcgccc agttctccgt
     3301 gtcgatcatc ctgccgttgc gatcgaacac gacgatgacg tgctcccagc gccagtcgac
     3361 gccgggccgg ccattgcttc cgttagccgc ggccctgccg aagctcggtg tcgcgctggc
     3421 ggtttcgcgt acaggcagac cgcccccgac ggggaacttg atgctcggtc caatctgcgg
     3481 aagccaggtg gtcccgcgcc cgagttgaag gaccggcaac tcgcccttct gcgtcacgaa
     3541 gacccggtcg ggactctcgg gaaaaatgtc ggtggactgc gaccatgtcc aggcctgatg
     3601 gtcgggaaga ctctctccca tgggccgggg ccagtttgcc accggatcgt agggaccgaa
     3661 cgcgtcgttc ccgcccttga gtcccggcac ggcggcgaaa ccctgcccgg gcgttctctt
     3721 ctgctgtccc tggacagcgc ccatcctgtt gctcaacagg atgaccacgg ataacgcgat
     3781 gcacgctgcc gcaatcttga cgagtcgctc catgtcgatg cctccgcggt gctcggattg
     3841 acgctgcgat catcctcccg cgggctcgaa tgtcaagaac tcctcggtgc gctagtcctg
     3901 agtcggtgcc tcgacgctgt cgatgaccag cacgtcaacc ggaccacggg tcgactcgag
     3961 cttcagcccg agctgttccc tgagcgccgt catcaccgac gagccatccg actgcaggtt
     4021 cgacagtgcg gcagtcggca cgtccacacc cagcttctgg acgagtccca ggagcgcctg
     4081 catgtcgagc ttcatgtcga agtcatagag gcccgtcagg cccgttttgt cgcggacgtg
     4141 cttgccggtg aattgcgaca gcaggtccac cagtaccttg atctcctggc cgtcgccgtg
     4201 cagcgacagg ttggccggac cgccggccag gttcggggcg atcgtgcagg cgaagaactc
     4261 acccggcttc ggaatgagcg ccgcgaagtc gcctttcgcc tgtccctcgg cccgcgtggc
     4321 gttcagctcg tcggcgttgc tgcaatccga cttcgatggt ttcagctcgg gcccggtttg
     4381 gccgtcgctg cgtgcgagca cgaggtcata gtgcgacatc tcgcgcggct cgaagtggaa
     4441 cttcaactgg aaccgctcgg tgatcaggtt cttcagcatc ggcagcgccg ctttcattcc
     4501 aagcgtggcc ccgccctccg tcttcgccgt gatgtggtac ttggtgttca tcaactcctt
     4561 gtcacccccg acgatccgga agtccggcaa ctcccaggcg gtggagatga gcgcccacag
     4621 cggcatattg gtcgccgtga accgtccacc gggccgcggc gccgccatcg gcatcatgct
     4681 cagcgggttc gatggatcgc ctggcgggct cggcttgacg gacgccacct cgaaggtcgt
     4741 ggcgggcgct gcggcggccg ggggcgccgg gggttgctgc gcagacactg ctgtcgcagc
     4801 gagcagggcg gcgaaggcga atgcagacgt cgagcgaata ctgttcatgg tcaaccttcc
     4861 agctttctcc aaacgttcgt agtgtccggc tttcctggcc tgagcgaagt cgaaggcagc
     4921 cggaccgtga tcagtaagag ctctggttat tgttcacacg ccaccttgta tctcccggct
     4981 atcgaaaccc ctgtcgcggt gatctcgagg tgcgcagccg caccgagcca cggagcgtcc
     5041 acctcgatgc tctctttcaa ttccgttgtc ttcgactgga cggcctgagc cgacgccacg
     5101 ggactctccc gcagtccggc cgcgccaaag ccaatcgcag tgcctgcgat cagaagaccc
     5161 acgcccgtcc atcgattcaa catggcgttc tccaatctcc ctcacgtatg gcgagcgcgg
     5221 ctgaatgacc acggcgccag gacgctggtc atcaacggca ggagcgcggg cgacagcatc
     5281 cagacgacgt ccatcgccgg catcccggac agctgcacgc cggccgctcg cagcacgatc
     5341 acccaggtcg ccccgaatgc ggccgagacc agcacggcac cagacgcctg ccccagcgtc
     5401 agcagccgct cggcaaagga actcgagacg aggtagaccg tcgcgagcac aattgccgct
     5461 acggccagcc actttgtcgc cctgagcatc gcgggcaaca ggaactccgg tccgatcccg
     5521 cgctggccgg ccagcgccag caggacgagc gcaagaccat ggagcaacag cagcgatgac
     5581 gcggtgttca cccggcgggg atagcgtgtc gcaagtgctc cgagcgccgc ccacgacgcc
     5641 acccagatgg cgaccccggt gaacgcgacg gctgccagcg cgagcttctc gtagcctgtc
     5701 agcgcgccga cggcgccttc aatcgcacgc tgccagttct cgagaggctc gtcggcgatc
     5761 tcgacgaggg gtgcgtagcc cttgtcgacc gcgatgaatg actgggatgc ccacacactc
     5821 acgccgacgg cgaagatcgc ggccaactgg cagaccgacc tcaccagcac tttgaggccg
     5881 gccagccggg cagtgccgta cgcctgggtc gcctcgaatg cgctggcgta cctgtgtcca
     5941 ggtcgccagc gaataccgaa ggcatttccc ccgaggccca gcacggtcac gacggagagg
     6001 atcgcaaaga gcattgccaa cgctcgagcg tagaagcatc cactgtctgc gcaaggcaca
     6061 tactgtcgaa ccccgtcgta gagagcggca tcgatggggc cgctgaccgc gaacagcaat
     6121 ggattcacga ttgcgagcgc cgctccgatc gccagcagcg gcagtccccg ggacctcagc
     6181 tcgaaccaca cctgcgcacg agttgcagac gagatggggc acgggaaacg gaacaggctg
     6241 acgagccggt ctggaaatcc gctgcctgga atccacgaga tggccgcgcg cccgtcgccg
     6301 cggcgctggc gcgcaacagc ggcgaccgtc agaccgtacg acgccaggcc aattgcgccg
     6361 atcagcgcgt agcaggtgag tggcaagtcg aacaccgtcg gccacaggtg cagcggcgca
     6421 aagcccgggc ccgggatctc ttcgttgagg agatatatag gcgcagcgct ccaggccacg
     6481 ctggcagcca ggtgtgccag tgttcggacg gccacattcc ggatcgacca gttcaccgcc
     6541 cacgaggtca ggttgagggc cgtgatccac gcggcggcgg gcagcagcgg gaacgcatgg
     6601 ccggaggtga ttctcaggag aagcgccgac acgaggtatg acgcagccgg caaggccgcc
     6661 aggtacgcca tcggaatacc gacggccact gccgtccgaa ccgggcgggt gtagaggaga
     6721 tgaaacggaa agcccgctcg ttcgaccatg agcttgttga cagagaacca cccgatgacg
     6781 tgcggcacga agacgacgat gagagccatc accgcccagc gatccttggc cgcttcgttc
     6841 ccggcgacgg cagcgacggc cgcgagcgcc actagtacgg ccgaggccgc gacgattccc
     6901 agcgccaggt gccaggcggc ttcggcgcgc gtcagccgcc agttctccca cagcatcgcg
     6961 atgatgggtg accgcatggt catgccgcct ccacctgctg gcggctgcgg ccggcgcggg
     7021 ccaggaagat ccatgtctat ctctccagtt ccgccgtcaa cgtggcctag cgcagtacga
     7081 actgctcgtt ctcgatctcg atgtcgagct cgacgtgctc gatctcgtaa actgtccggc
     7141 ccaactgctc gttcgactcc acctggcgga acggcagccg cactccgtcg acgaggcggt
     7201 agtcctcgaa ccgcgtggtc gatggcactg gaccgagtcc cggcatgacg agcgtcgtgg
     7261 tgagcgcgag cagatcgccg gactccgcgt cgaccctcag cgtgacgttg ggcagc
//
LOCUS       DBNGSH010000848         1558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3497395, whole genome shotgun sequence.
ACCESSION   DBNGSH010000848 DBNGSH010000000
VERSION     DBNGSH010000848.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3497395"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC16CY_19490"
     CDS             complement(<1..347)
                     /locus_tag="AC16CY_19490"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3356043.1"
                     /translation="MPAPTTRIVRRASQSTPSTIAADICVLGAGIAGVSAALEAARAG
                     RTVVIADAASALGGQAIGSIIGTIIGLYTHGAEPYQITHGIADDLITELTAEGALNRR
                     VSMTGTITFQYDEV"
     gene            457..>1558
                     /locus_tag="AC16CY_19495"
     CDS             457..>1558
                     /locus_tag="AC16CY_19495"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3356044.1"
                     /translation="MNNWLRGSIVSGALGVTLVLAVDIPRAQQAAPPASNASTCQPPA
                     AAGGARGARAGGRAQGARGAQAPAGGAGQAGPAGAARRGGGGGNPPPIAWAAPPLPDG
                     PIMVESAVVAHRNLRLVVTKGLSHPWGMAFLPDGNILITERPGCLRIVRNGVLDPKHV
                     AGLPPISAQGLAGLLDIALHPQFAQNKFVYLTYHKPNPAFTAGAPAQGSVPAQPGPPQ
                     RLATLARGVWDGNALTNVRELFSSDVTTEASRIVFGRDGMIYMSLGRSQEGANAPSQD
                     PSNYGGKLLRLRDDGTVPPDNPFVGRPGYKPEIFTMGHRNQLGLAVNPETGEIWASEQ
                     GPNGGDEINVIQAGRNYGWPVVSYGRSYPGPRV"
BASE COUNT          251 a          506 c          557 g          244 t
ORIGIN      
        1 acctcgtcgt actggaacgt gatggtgccc gtcatggaga cccggcggtt caaggcacct
       61 tccgccgtga gctctgtgat caaatcgtcg gcgatgccgt gagtgatctg atagggctcc
      121 gcgccatgtg tgtacagccc gatgatggtc ccgatgatcg acccgatggc ctgccctccg
      181 agggcggatg ccgcatcggc gatgacgacc gtccgtccgg cgcgcgcggc ctcgagtgcg
      241 gccgagacgc ccgcgatacc ggctccgaga acgcagatgt ccgccgcgat cgtcgagggc
      301 gtggattgag aggcacggcg cacgatgcgc gtggtcgggg cgggcattcc ctatgagtgg
      361 gatgagaata caccggggat atacttacgc gttcgtgacg atgcgcttgc ctcgctgacg
      421 gagcggagcg gcgaaggccc tggcgggaga acgacgatga acaactggct gcgcggttcg
      481 attgtgtcgg gggcgctggg tgtgacactg gtgctcgccg tggatattcc acgcgcgcag
      541 caggccgctc cgccggcgtc aaacgcgtcg acgtgtcagc cgccagcggc ggccggtggc
      601 gcgcgcggcg cccgtgcagg cggacgggcc cagggcgcgc gaggcgcgca ggcgcccgcg
      661 ggtggcgccg gtcaggccgg tcccgctgga gcggcccgcc gcggtggcgg cggagggaac
      721 ccgccgccga tcgcatgggc tgcgccgccg ttgcccgacg gcccgatcat ggtcgagtcg
      781 gccgtcgtcg cgcaccgcaa tctgcggctg gtcgtgacca aggggttgtc gcatccgtgg
      841 ggcatggcct ttctgcccga cggcaacatc ctgatcaccg aacgcccggg ttgtctgcgg
      901 atcgtgcgca acggcgtgct cgatccaaag cacgttgccg gccttccgcc gatctcggca
      961 caagggctgg cagggctgct ggacattgcg ctgcacccgc agttcgcgca gaacaagttc
     1021 gtctatctca cgtaccacaa acccaacccg gcgttcactg ccggggcacc cgctcagggc
     1081 tcagtgccgg cacagcccgg tccgccgcag cgtctcgcga cgcttgcgcg aggggtatgg
     1141 gacggcaacg cgctgaccaa cgtgcgtgag ctcttctcca gcgacgtcac gacggaggcc
     1201 tcgcgcatcg tcttcggccg cgacggcatg atctacatga gcctggggcg atcgcaggag
     1261 ggggcgaatg cgccgtcgca ggatccaagt aactacggag gcaagctgct tcgtcttcgc
     1321 gacgacggca ccgtgccgcc ggacaacccg tttgtcggac gcccaggcta caagcctgaa
     1381 attttcacga tggggcaccg caaccagctc gggttggcgg tgaacccgga aaccggcgag
     1441 atctgggcga gcgagcaggg cccgaacggc ggcgacgaga tcaacgtgat ccaggccggc
     1501 aggaactacg gctggcccgt cgtcagttat gggcgcagtt atcccggccc ccgcgtgt
//
LOCUS       DBNGSH010000849         2156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_440959, whole genome shotgun sequence.
ACCESSION   DBNGSH010000849 DBNGSH010000000
VERSION     DBNGSH010000849.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_440959"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..551)
                     /locus_tag="AC16CY_19500"
     CDS             complement(255..551)
                     /locus_tag="AC16CY_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3356045.1"
                     /translation="MKTIPVDEYILDSLMPDLVGHDRQPSAFLVYLTLWRQTHGGGQA
                     ATQVALQDIAEATGLSKRSVQDALGRLARRRLISVSRASITAVPVYTVLRPWRR"
     gene            696..1130
                     /locus_tag="AC16CY_19505"
     CDS             696..1130
                     /locus_tag="AC16CY_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356046.1"
                     /translation="MTSRIRWGRVLIAAVMSDAGVIALLFAAITTYTSMTPGLTDDQY
                     YALGEDIGYYVAPTAGAILTAFSVLWAARRLTSAFVLHGVLIGVVATLLTVGFIFSAR
                     PEHQVMYIVSFGLKIAGGYVGGLVAQWRFNARTAPAPESHAV"
     gene            1140..1505
                     /locus_tag="AC16CY_19510"
     CDS             1140..1505
                     /locus_tag="AC16CY_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3356047.1"
                     /translation="MIKGVKFVTIPVRDQDRALEFYTKKLGMRVITDSPYDDTQRWIE
                     LGIPRADTKVVLFTASAHEQMIGGVMNITFMAEDVVATASALKARGVEFVQEPQKSDW
                     GTAAVFKDPDGNVFALSTP"
     gene            complement(1553..1981)
                     /locus_tag="AC16CY_19515"
     CDS             complement(1553..1981)
                     /locus_tag="AC16CY_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR3356048.1"
                     /translation="MEDTRYGLRVEIPGSYEEAIERATEALKAQGFGVLTTIDVRQTL
                     KQKLNRDFRKYVILGACNPPLADRALHAELEVGLLLPCNVIVYELAPGRSVVSAMAPL
                     AAMGVVGDNRELQAVAGEADRRLRAALVTLETEVAQLTPR"
BASE COUNT          374 a          729 c          682 g          371 t
ORIGIN      
        1 ggcgctaatc ccggagttca cttgaattca gtctgagatc tgtcgttcac gcttctccat
       61 tctcgcttct ctgttcgtgt tcaagttcgc gggtgccgca gcgcgcgacg caccccgtgc
      121 aaacgccgaa cagcgaaccg agaaccgaga accgaacgtg aacacgaacc gaaaaggtta
      181 gaaccgagaa gtgtgaacgg ctgatctctt gctcgacaca agtgaatccg ggttgtggtt
      241 gctcccgacg acgatcagcg tctccagggc cggaggaccg tatagaccgg cacggccgtg
      301 atgctggcgc gcgacacact gatcaacctg cgcctggcga gccgtccgag cgcatcctgc
      361 accgaacgct tcgagagccc cgtcgcttcc gcgatgtcct gcagcgcgac ctgcgtcgcc
      421 gcctggccgc cgccgtgggt ctgccgccac agagtcaggt acacgaggaa ggcggacggc
      481 tggcgatcgt ggccgacgag gtctggcatc agcgagtcga ggatgtactc gtccacgggg
      541 atggtcttca cgccgggcgt cctccgatat ccatactggc aacggctgcc atagtagtga
      601 atgcacgcct tgcgctcaac agcggatccg cccatcatgt cgtccgggcc gcacgtccat
      661 cgagcggctc gatggcccta atccggaggg agggtatgac gtcacgcatt cgctggggac
      721 gcgtgctcat cgcggcggtc atgtccgacg cgggcgtgat cgcactgctg tttgccgcga
      781 tcacgaccta cacgtccatg acaccgggac tgaccgacga ccagtactac gcgctgggcg
      841 aggacatcgg ctactacgtg gccccgacgg cgggcgcaat cctgactgcc ttttccgtgc
      901 tctgggcggc gcggcgactg acgtcggcct tcgtcctgca cggcgtcctc atcggcgtgg
      961 tcgccacgct cctgaccgtc ggcttcatct tcagcgcgcg gcccgagcat caggtgatgt
     1021 acatcgtctc cttcgggctc aagatcgccg gcgggtacgt cggcggcctg gtcgcccagt
     1081 ggagattcaa cgccagaacc gcgccggcgc cggagagtca cgcagtctag gggggcgcga
     1141 tgatcaaagg ggtcaagttc gtcaccattc cggttcgcga tcaggatcgc gcgctcgagt
     1201 tctacacgaa gaagctcggc atgcgtgtca tcacggacag tccgtacgac gacacgcagc
     1261 gctggatcga gctcgggatt ccgcgcgccg acaccaaggt cgtgctcttc accgcgtcgg
     1321 cgcacgaaca gatgatcggc ggcgtgatga acatcacgtt catggccgag gacgtcgtgg
     1381 cgacggcgtc ggcgctcaag gccaggggcg tcgagttcgt gcaggagccg cagaagtccg
     1441 actggggaac ggccgccgtc ttcaaggacc ccgacgggaa cgtgttcgcg ctgtcgacgc
     1501 cctgagcgct cctcgcgctc cggctcgcgc ccggcgccgg gcgacgtctt cctcaacgcg
     1561 gggtgagctg cgcgacctcc gtttcgagcg tcacgagcgc ggctcgcagc cggcgatcgg
     1621 cctcgcccgc aacggcttgc agctcgcggt tgtcgccgac cacgcccata gcggccagcg
     1681 gcgccatcgc cgagacgacg ctgcgtcccg gtgcaagctc gtacacgatg acgttgcacg
     1741 gaagcagaag gccgacctcg agctcggcgt gaagagcacg gtccgccaac ggcggattgc
     1801 atgcacccag aatcacgtac ttgcggaagt cgcgattgag cttctgcttc aacgtctgcc
     1861 ggacgtcgat cgtcgtcagc acgccaaagc cctgagcctt gagcgcctcc gttgcccgct
     1921 cgatcgcctc ctcgtagctc ccggggatct ccacgcgcaa tccgtaacgg gtgtcttcca
     1981 tgttctcctc caccacccag gtttgcccca tcggtgaatc ggcagccgac ggccgcaggg
     2041 agctgaactc agcccacgac cggcacgtca tgccacttga cggcaccccg ttgctcccgc
     2101 gcaaagtcct ccagccgacg gcgggctgca tcgaatgcct cgcggacggc cacggc
//
LOCUS       DBNGSH010000850         3599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4177838, whole genome shotgun sequence.
ACCESSION   DBNGSH010000850 DBNGSH010000000
VERSION     DBNGSH010000850.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4177838"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="AC16CY_19520"
     CDS             complement(<1..525)
                     /locus_tag="AC16CY_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356049.1"
                     /translation="MSGPSGVLHDLRYAIRLFRNSPSFSAVAIATIALAVGANTAMFS
                     FVNGLLLRPLPYPDAERIVRVLERLPGGGPNSISTLNYLDWTNQSTVFEFMAAEAGWS
                     ATLSGGDEPVLIRAARVSAQYFDIFGVTPALGRTFLPGEDQPGSDRVVLLSHVLWERR
                     FGSDPAMLGRDVVLN"
     gene            591..989
                     /locus_tag="AC16CY_19525"
     CDS             591..989
                     /locus_tag="AC16CY_19525"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3356050.1"
                     /translation="MTRAIVTSFMLAAAILQAVPASPHHSNAAYEVTKVITITGVVKS
                     FEWVNPHTWLTVVVDDGKGGKVEWACEGRAPGILTRAGWTRSVLKAGETVTVDMSPAK
                     DGSKVSIIARVTKADGTILSNQPQFDPRGN"
     gene            991..1779
                     /locus_tag="AC16CY_19530"
     CDS             991..1779
                     /locus_tag="AC16CY_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356051.1"
                     /translation="MFRLIAVLAIAVLSAPSLPAQATPDFSGVYYPAQQGRGGGGGQR
                     GAQPAAGAQRGAPPPRPTQSAPVSDGSQGRSADAPSLTPEYLAKWELMRKSRVAGSYE
                     YDNNAKCLPPGMPAMMNMAYGMEIMQTKDRITFFSELNDALRRVYLDGRKPSQKHLDD
                     PTYAGYSTGRWEGDTLVVDTVALHPDSFIEGFTPHSDAMTVHERIRLIAPDVLEDRIT
                     VTDPKALTKPWEVVRTYRRAAKGNDELREFACAEGLSRELLKGK"
     gene            complement(1789..>3599)
                     /locus_tag="AC16CY_19535"
     CDS             complement(1789..>3599)
                     /locus_tag="AC16CY_19535"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3356052.1"
                     /translation="RRAADALPPGLSAMWRLCKLGYSHEPGLLLAAFMLALLGALPDA
                     LLALWFKMLGDGALQRNWSLVQFAAVALGVSATATWFLQTVSTRVQRRFRDKVTIALE
                     SHVATLLASISTIAHQERPEYLDRLAVLRNQVFMLDHMYMSLFTTCGWILRLGVTIAL
                     LVSIHPALALLAIFAIPTVMTSTWRPAVERRAYERGAQANRLARHLFDVATTATPGKE
                     VRVTGIGDRLKTERRMAWEFGYRPIAAARSASAAWHTLAWAVFGAAYVGAIVFVSSGL
                     QAPVGDVLLALAAGSRLSFYVGATVGEIGFLRGIWLDAARRLAWLEDYASSLAASADQ
                     SAPERLAEGIRFDHVSFAYPGTDRLVLDDVSLTLPAGAVVAIVGENGAGKTTLMKLLA
                     KMYEPTRGRILIDRTDLARIPAADWRDRLAGAFQDFFRFEFRARHSVGVGDVPRLDDE
                     PAVVAAVGRAGANDVVERLPSGLETQLGPAWPGGVDVSFGQWQKLALARGFMRDEPLV
                     LVLDEPTAALDAETEHALFERYAAAAGGEGNGGHGGSRGRITILVSHRFSTVRMAGLI
                     VVLDGARVVEVGTHEALMARQGQYAQLYGIQAAAYS"
BASE COUNT          645 a         1210 c         1237 g          507 t
ORIGIN      
        1 gttcaacacg acgtccctgc ccagcatcgc gggatcggag ccgaagcggc gttcccagag
       61 cacatggctc agcagcacga cacgatcgct gccgggctgg tcctcgcctg gaagaaacgt
      121 gcggcccagc gctggcgtca ctccgaagat atcgaagtac tgcgccgaga ctcgcgcggc
      181 ccggatgagg accggctcgt ccccgccgct caatgtcgcg ctccagccag cttccgcggc
      241 catgaactcg aagacggtgc tctgattcgt ccaatcgaga tagttcagag tagagatgct
      301 gttgggcccg ccccctggaa gcctttcgag gacgcgcacg atgcgctcag cgtcgggata
      361 tggcagagga cgaagtagaa ggccgttcac gaagctgaac atcgccgtgt tcgcgccgac
      421 cgcgagcgca atggtggcaa tcgcgacagc cgaaaagctc gggctgttgc gaaacaggcg
      481 gattgcgtaa cgaaggtcgt ggaggacgcc agaagggccg ctcatgaggc tcccgtcgcg
      541 ccatggcagg cgcgcacgta taatccgtca gtttcgtcca ggaggagcaa atgacccgcg
      601 cgatcgtcac ttcgttcatg ctcgccgcag cgatcctcca ggctgttccc gcgtcgcctc
      661 accacagcaa cgccgcctac gaggtgacca aggtgatcac catcaccggg gtcgtcaagt
      721 ccttcgaatg ggtcaacccg cacacctggc tcacggtggt cgtcgacgac ggcaaggggg
      781 gcaaggtgga atgggcgtgc gaaggacgcg cgccgggaat tctcacccgg gcaggctgga
      841 cgcgctcggt gctgaaggcg ggtgagaccg tcacggtgga catgagcccc gccaaggacg
      901 gttcgaaggt gtcgatcatc gcgcgggtga cgaaggccga cggcacaatc ctgtcgaatc
      961 agccgcagtt cgacccgagg ggaaattgag atgtttcgcc tgatagcggt gctcgcgatc
     1021 gcggtgctgt cggctccgtc gctgccggcg caggcgacgc cggacttctc cggcgtctac
     1081 taccccgcgc agcagggccg tggaggtggt ggaggacagc ggggcgccca gccggcggca
     1141 ggcgcgcagc gcggtgcccc gccgccgcgc cccacgcagt ccgctccggt gtcggacgga
     1201 tcgcagggcc gctccgccga tgcgccctcg ctcacgccag agtacctggc gaagtgggaa
     1261 ctgatgcgga agtcgcgcgt ggcgggctcg tacgagtacg acaacaacgc caagtgcctg
     1321 ccgcccggca tgccggcgat gatgaacatg gcgtacggca tggaaatcat gcagacgaag
     1381 gatcggatca ccttcttcag cgaactgaac gacgcgctgc ggcgcgtcta cctcgacggc
     1441 cggaagccat cgcagaaaca tctcgacgat ccgacgtacg cgggctactc gacgggccgg
     1501 tgggagggcg acacgctggt cgtcgacacc gtcgcgctgc accccgactc gttcatcgaa
     1561 ggcttcacgc cgcacagcga cgcaatgacc gtccacgagc ggatccgtct gattgccccg
     1621 gacgtgctcg aggatcggat taccgtcacc gatccgaaag cgttgaccaa gccatgggag
     1681 gtcgtccgga cgtatcggcg ggccgcgaag gggaacgacg agcttcggga gttcgcgtgc
     1741 gcggagggac tgagccggga gctgttgaag ggaaagtaag gagccgggct acgagtacgc
     1801 cgcagcctgg atgccataca gctgcgcgta ctgaccctgc cgtgccatga gcgcctcgtg
     1861 cgtcccgacc tcgacgacgc gagcgccgtc cagcacgacg atcaacccgg ccatccgcac
     1921 cgtcgagaag cggtgcgaga ccagaatcgt gatgcggccg cggcttccgc cgtgcccgcc
     1981 gtttccttcc ccgccggcgg cagccgcgta gcgctcgaag agcgcatgct cggtttcggc
     2041 gtcgagcgcg gccgtcggct cgtcgaggac gagcacgagc ggttcgtcgc gcatgaatcc
     2101 gcgcgccagc gccagcttct gccactggcc gaagctcacg tccacgccgc ccggccacgc
     2161 gggaccaagc tgcgtctcga gccccgaggg gagccgctcc acgacgtcgt tcgctcccgc
     2221 gcggcccacg gccgcgacga cggccggctc gtcgtccagg cggggcacgt cgcccacgcc
     2281 gacgctgtgg cgggcgcgga actcgaagcg gaagaagtcc tggaaggcgc cggccagccg
     2341 gtcacgccag tcggcggccg gaatgcgggc aaggtcggtc cgatcgatca ggatgcgtcc
     2401 cctcgtcggc tcgtacatct tggcgagcag cttcatcaag gtcgtcttgc ccgcgccgtt
     2461 ctcgcccacg atcgcgacga ccgcgccggc aggcagtgtc aggctcacgt cgtccagcac
     2521 cagtcgatcg gtacccgggt aggcaaagga cacgtgatcg aatcggatgc cctcggcgag
     2581 gcgctcgggg gccgactgat cggccgacgc cgcgagcgag ctcgcgtagt cctcgagcca
     2641 cgcgagacgc cgcgccgcgt ccagccagat gccgcggagg aagccgatct cccccaccgt
     2701 tgctccgacg tagaacgaca ggcgcgagcc ggctgccagc gcgaggagta cgtctcccac
     2761 gggcgcttgg agacccgagg acacgaagac gatcgctccg acgtacgcgg caccgaacac
     2821 cgcccatgcg agcgtgtgcc acgcggccga cgccgaccgg gcggcggcga tgggcctgta
     2881 cccgaattcc cacgccatcc gccgctccgt cttcaagcga tcgccgatgc cggtgacgcg
     2941 cacttccttg ccgggcgtcg ctgttgtcgc cacgtcgaag aggtgacgcg caagccggtt
     3001 cgcctgcgcg ccgcgttcgt acgccctgcg ctcgacggcc ggccgccagg tggacgtcat
     3061 caccgtgggg attgcgaaga tggccagcag cgcgagcgcg ggatggatgg agacgagcag
     3121 cgcgatcgtg acgccgagcc gcaggatcca cccgcacgtc gtgaagagcg acatgtacat
     3181 gtggtccagc atgaacacct ggttgcgcag cacggcgagc cgatcgaggt actcggggcg
     3241 ctcctggtgc gcgatggtcg agatcgacgc gagcagcgtg gcgacgtgcg actcgagcgc
     3301 gatcgtcacc ttgtcgcgga agcgccgctg cacgcgcgtg ctgacggtct gaaggaacca
     3361 ggtcgccgtg gcggacacgc cgagcgccac cgccgcaaac tgcacgaggc tccaattccg
     3421 ctggagcgcg ccgtcgccga gcatcttgaa ccagagcgcg agcagggcgt cgggcagcgc
     3481 tccgagcaac gccagcatga acgccgccag cagaaggccg ggctcgtggc tgtagcccag
     3541 cttgcagagc cgccacatgg ccgagagacc cggaggcaga gcgtcggccg cgcgccgcg
//
LOCUS       DBNGSH010000851         5242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3159818, whole genome shotgun sequence.
ACCESSION   DBNGSH010000851 DBNGSH010000000
VERSION     DBNGSH010000851.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3159818"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..408
                     /locus_tag="AC16CY_19540"
     CDS             262..408
                     /locus_tag="AC16CY_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356053.1"
                     /translation="MLFIVGVVIVLIAVVIILMMRAPSGVNSANLGYMSERWLAEHRT
                     SRSL"
     gene            complement(413..1492)
                     /gene="hemE"
                     /locus_tag="AC16CY_19545"
     CDS             complement(413..1492)
                     /gene="hemE"
                     /locus_tag="AC16CY_19545"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR3356054.1"
                     /translation="MTDSDKGPGAQGSGLRESSRFLKACRRQPVDMTPVWFMRQAGRY
                     MEEYREIRSRHSLLDICRSPDLATEVTLQPVRRLEVDAAILFSDLLLPLEPMGLPFDF
                     IKGEGPQLERPIEGPADIDRLRVFEPREALRHVLDTIRQVQHELDGRVPLIGFAGAPF
                     TLASYAIEGGHSNNFAKTKALMYSQPDAWHRLCQMLSGVVSSYLLAQIDAGVDAVQIF
                     DSWVGALDATDYREFALPHTRRIFDAVAGRVPTIHFGTGTATILEELRDAGGDVIGVD
                     WRIAIDEAWDRIGPGRAVQGNLDPTLLLGPPQRMLHQADALLRKVGGRAGHIFNLGHG
                     ILPSTPVEHVQLLAHHVHTASRKVR"
     gene            complement(1485..1901)
                     /locus_tag="AC16CY_19550"
     CDS             complement(1485..1901)
                     /locus_tag="AC16CY_19550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3356055.1"
                     /translation="MSAVSPGDGGASPARVLIVDDDASVAELFSRMLRLEGFEVWAAH
                     SADEGLGLAQTHRPHAVILDLRMPLASCLRVLRALRAIPGQQQTPVTIVTGDYYLGEA
                     QSDEIRTLGADVRFKPLWLDELVALARDMLPATVRD"
     gene            2164..2814
                     /locus_tag="AC16CY_19555"
     CDS             2164..2814
                     /locus_tag="AC16CY_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356056.1"
                     /translation="MSPILVRPVREQLEHDRVIRLLQAKYKRKFDVAINPGNEQAAPV
                     GSAAAPWYPDLVLQSGERSRKLLGVVEVETAESLNNLEAMSQWVAFSKLRVPFHLYVP
                     ASGVDHARRLCSDLQIQAAEMWAYSAIGEQIRFTLVARSPQAGDRIREPKPARPSAPE
                     PSRSKPSRPAASRAVRGAAASTRARTGAAKKKTADARGAKKATARQSAASRPQKRK"
     gene            2820..3482
                     /locus_tag="AC16CY_19560"
     CDS             2820..3482
                     /locus_tag="AC16CY_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356057.1"
                     /translation="MPFLRVIRDRRGYETTYLMHLYRDGHRQRSRILYVFRSPGGVRV
                     GRSALDPDVQRLIESQNPGITFDWKEVREGQQYIEMAPEPRRRKAPRPEEPAPVPPET
                     AVPAAAMPAAAVPARLEVPSSIEGATPDEQVAFLGRWYPLIRERIPRRTSDPLRQEAL
                     LALNERLNPAAWTDADQIAAGLQQAAVALERLSRVFAKRRKRARRRPQSPPSADQAPS
                     ES"
     gene            3479..4084
                     /locus_tag="AC16CY_19565"
     CDS             3479..4084
                     /locus_tag="AC16CY_19565"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR3356058.1"
                     /translation="MSDWIESVALALSDLGPWAAVVFVAAYVVASVTLAPAFLLTMAG
                     GAVFGLWRGTLLVFAGAVLGASAVFVIASPLSRSRLLRRLDRDPRVAAVRHAVTGEGA
                     WVMFLLRLSPAVPFVLLNYALALSGARYRDYLVASVGMLPAIVVYVYYGKVAGDVARV
                     AAGVVPPRGAEYYVLLAVGLAATVAATHAITKAARNAMKSR"
     gene            complement(4095..>5242)
                     /locus_tag="AC16CY_19570"
     CDS             complement(4095..>5242)
                     /locus_tag="AC16CY_19570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3356059.1"
                     /translation="DQHLDDLDALVTELHRSLPAGSASDADDALTLATWLGQVRTMDG
                     SDLLLVAGQPPLVKIKGVVSPLPGHSLTGEEIESVVSSVVPFRLRERYRAGEAIDLAF
                     TLRGFGRFRVNLHRERGHAAAAIRALPTRIPRIPELHFSHDIAILAELPRGLVLVCGP
                     MGSGKTTTLAALIAEINARYSRHIVTVEDPIEYEHPHGRCVIEQVEIGLDAPDFPTAL
                     RSAVRQAPDVIVVGEMRDPETMRIALSAAETGHLVLATVHATDVAAAVSRIADGFPVE
                     RQNAVRQELAMALAAVLTQSLVPTRTGTIVPVAELLLVSYGARQHIRKNALQHLNQEI
                     TITRKAGSFTLEESLARFVQNGVLDRAEAMLRAAHAEELESLLRPRA"
BASE COUNT          861 a         1706 c         1796 g          879 t
ORIGIN      
        1 aatcgccaaa accatcacag tcaagacctt gtggcgctgc cgagagtgcg aagaaacatg
       61 gacgatcgcg agccgtgcag catctcaggc acgttcgcct tccactttca gataggcggg
      121 cgcgcagagg ccctacgtcc gaaccagatc gcgcacacaa cccccgggtt cacatgcctg
      181 cggtgtgcac aaacgcacag actgtcacct tgccgcgtgt caggatcgct agacgccgac
      241 ctgaatgcgg cgagaaccca catgctgttc atcgttggcg tcgtcatcgt gttgatcgcg
      301 gtcgtcatca tcctcatgat gcgggcgccg agtggcgtga actccgccaa tctcggttat
      361 atgagcgagc gatggctcgc tgaacatcgt acgtcgcgct cactgtaaag aatcaccgca
      421 ccttgcggga cgcggtgtgc acgtggtggg ccagcagctg gacgtgctcg acgggcgtcg
      481 atggcaggat gccatgcccg aggttgaaga tgtgtccggc acggccgccg accttgcgga
      541 gcagcgcgtc ggcctggtgc agcatgcgct ggggcggccc gagcagcagc gtcggatcga
      601 ggttgccttg taccgcgcgg ccgggtccga tccgatccca ggcctcgtcg atcgcgatgc
      661 gccagtccac gccgatcacg tcgccgccgg catcccggag ctcctcgaga atcgtcgccg
      721 tgcctgtgcc gaaatgaatg gtcgggacgc ggccggccac ggcgtcgaag atgcggcgcg
      781 tgtgcggcaa cgcgaactcc ctgtaatcgg tcgcgtccag cgcgccgacc caggagtcga
      841 agatctggac cgcgtccaca cccgcgtcga tctgcgcgag caggtagctc gacacgacgc
      901 ccgacagcat ctggcagagc cggtgccagg cgtcgggctg gctgtacatc agggccttgg
      961 tcttggcgaa gttgttcgag tgcccgccct cgatcgcgta ggaggcgagc gtgaacggtg
     1021 cgcccgcgaa accgatgagc ggcacgcggc catcgagctc gtgttgcacc tggcggatgg
     1081 tgtcgaggac gtgccgcagc gcctcgcgag gctcgaagac gcgaagccga tcgatgtcgg
     1141 caggaccttc gatcgggcgt tcgagctgag ggccctcgcc tttgatgaaa tcgaagggga
     1201 gccccatcgg ctcgagcggc agcagcagat ccgagaacag gatggccgcg tccacctcga
     1261 ggcgccgaac cggctgcagc gtgacctcgg ttgcgagatc cggcgagcgg cagatgtcga
     1321 gcagggagtg ccggctgcgg atctcccggt actcttccat gtatcgcccg gcctggcgca
     1381 tgaaccagac aggcgtcatg tccaccggct gtcggcggca ggccttcaga aaccgggagg
     1441 actcccggag gcctgagccc tgggccccgg ggcctttgtc cgagtcagtc acggaccgtg
     1501 gcggggagca tgtcgcgcgc cagcgccacg agctcgtcga gccagagggg cttgaaccgg
     1561 acgtcggcac ccagcgtccg aatctcgtcc gactgcgctt caccgaggta gtagtcgccc
     1621 gtgacgatcg tgacgggcgt ctgttgctgt cccgggatgg cccgcagtgc gcgcagaacc
     1681 cgaaggcagc tggccagcgg cattctcagg tcgagaatga ccgcgtgagg gcggtgggtc
     1741 tgggcgaggc caagcccttc gtctgcggaa tgggcggccc agacttcgaa gccctcgaga
     1801 cgaagcatcc gtgaaaacag ctccgcaacg ctggcgtcat cgtccacgat cagcacgcgt
     1861 gcgggcgagg cgccgccgtc tcccggagac actgcgctca ttgttatgcc ccggtgggga
     1921 cgcgtcaagt agacgaaaca gcgaggagat ctggcggttc aagccgcgcg gctttcgtgc
     1981 tatattcttg cgcttctgtc ggcgcgtgca gtcatcctag tgggctgtat caactggaag
     2041 agtaccgcga acaacgagct tcgaagcacg atttccggcc gcgatggaca taccactaga
     2101 cggtcgcgag gcacaccagc gcgccggcga tagccgtcgc gtcttccccc gtcggaggtt
     2161 ctgttgagcc ccatcctcgt gcggcccgtg cgcgagcaac tcgagcacga tcgggtgatt
     2221 cggctgctcc aggccaagta caagcggaag ttcgacgtgg ccatcaatcc cggcaacgaa
     2281 caggcggcgc cggtagggtc ggcggccgcg ccgtggtacc ccgatctcgt cctgcagtcg
     2341 ggcgagcggt ccaggaagct gctcggcgtc gtcgaggtgg agaccgccga gtcactcaac
     2401 aacctcgagg cgatgtcgca atgggtcgcc ttctcgaagc ttcgcgtccc gttccacttg
     2461 tatgtgcccg cctcgggtgt ggaccacgcc aggcgtctgt gctcggacct gcagattcag
     2521 gctgcggaga tgtgggcgta ttcggccatc ggagagcaga tccgattcac gctcgtcgcg
     2581 cgctcgccgc aggcaggcga tcgcattcgc gagccgaaac ccgcgcggcc gtccgcgccg
     2641 gagccgtcgc gttccaagcc gtcgaggcct gccgcgtctc gagctgtgcg cggtgctgcc
     2701 gcaagcacgc gtgctcgaac cggtgcggcg aagaagaaaa ccgccgacgc tcggggcgcg
     2761 aagaaggcga ccgcacgcca gtccgccgct tcgaggcccc agaagcgcaa gtagccgctg
     2821 tgcctttcct gcgcgtcatt cgcgataggc gcgggtacga aaccacgtac ttgatgcatc
     2881 tctatcgcga cggacatcga cagcgctcac gcatcctgta cgtgtttcgg tcgcctggcg
     2941 gcgtgcgcgt cggccgcagt gccctcgacc cggacgtcca gcggttgatc gagtcgcaga
     3001 atcccggaat caccttcgat tggaaggaag tcagggaggg ccagcagtac atcgaaatgg
     3061 cgcccgagcc gcggcgacgg aaagcgccca ggccggaaga gccggccccg gtcccgcccg
     3121 agaccgcggt ccccgcggcg gcgatgccgg cagcggccgt gccggcgagg ctggaggttc
     3181 catcgtcgat cgagggtgcg acgcccgacg aacaggtcgc ctttctcggc cggtggtatc
     3241 cgctgatccg cgagcgcatt cccaggcgga cgtcggaccc gctgcggcaa gaggcgctgc
     3301 tggccctgaa cgaacgcctc aaccccgccg cgtggaccga tgccgatcag atagccgcgg
     3361 gacttcaaca ggcggcagtg gcgctcgagc ggctgtcgcg cgtgtttgcg aagcgccgga
     3421 agcgtgcgcg acgccgtcct cagtccccgc cttcggccga tcaggctcct tctgaatctt
     3481 gagcgactgg atcgaatcgg tcgcgctcgc gttatccgat ctgggaccat gggcggctgt
     3541 cgtattcgtt gcggcctacg tggtcgcgtc ggtcacgctt gcgccggcgt ttctgctcac
     3601 catggccggc ggcgccgtgt tcgggctgtg gcgcggaaca ctgctcgtct ttgccggcgc
     3661 cgtcctcggg gcgtccgcgg tgttcgtcat cgcgtcgccg ttgtcgcgct cgcgcctgct
     3721 tcggcggctg gaccgcgatc ctcgcgtagc cgccgtccgg cacgcggtca caggcgaggg
     3781 agcgtgggtg atgttcctgc ttcgcctgtc tccggccgtg ccgttcgtcc tgttgaacta
     3841 cgcactcgcg ctgagcggcg cgcgataccg ggactatctc gtggcgtcgg tgggcatgct
     3901 gccggcgatc gttgtgtatg tctactacgg caaggtggcg ggggacgtcg ccagggttgc
     3961 cgcaggtgtc gtgccgccca gaggtgccga gtactacgtc ctgctcgcgg tgggactggc
     4021 ggccactgtt gccgccacgc atgcaatcac caaggcggca cgcaacgcga tgaagagccg
     4081 ctgacgcgcg atggtcacgc gcgggggcgg aggagagact ccagttcctc cgcgtgggcc
     4141 gcccgcagca tcgcttcggc cctgtcgagc acgccgttct gcacgaacct cgcgagcgat
     4201 tcctccagcg tgaacgagcc agccttgcga gtgatggtga tttcctggtt caggtgctga
     4261 agcgcgttct tgcggatgtg ctgccgggcc ccgtagctga cgagcagaag ttcggcgacc
     4321 ggcacgatcg ttcccgtgcg cgtcgggacg agcgactgag tgagcacggc tgccaacgcc
     4381 atggcgagct cctgccggac ggcgttctgc cgctcgaccg ggaatccgtc ggcgatccgc
     4441 gataccgccg cggcgacgtc tgtcgcgtgc acggtggcca gcaccaggtg accggtctcg
     4501 gccgccgaca gcgcgattcg catcgtctcg ggatcgcgca tctcgccgac gacgatcacg
     4561 tcgggcgcct gacggacggc ggaacgaagc gcggtaggga agtctggtgc gtcaagcccg
     4621 atctccacct gctcgatcac gcaccgaccg tgggggtgtt cgtactcgat ggggtcttct
     4681 accgtgacga tgtgccgcga atacctcgcg ttgatttcgg cgatgagtgc ggcaagcgtc
     4741 gtggtcttgc ccgatcccat agggccgcag accagcacca atccgcgggg cagctccgcg
     4801 aggatcgcaa tgtcgtgcga gaagtgcagc tcgggaattc gagggattcg tgtcggcagc
     4861 gcgcggatgg ccgccgccgc gtggccgcgc tcccggtgaa ggttcacgcg gaagcggccg
     4921 aaccctcgaa gcgtaaaggc cagatcgatc gcctcaccag cgcggtaccg ctcgcggagg
     4981 cggaacggca cgacggaaga cacgacgctc tcgatctcct caccagtcaa gctatgtcct
     5041 ggcaggggcg acacgactcc cttgatcttg acgagcggcg gctggcccgc cacgagaaga
     5101 agatcgctcc cgtccatcgt gcgcacctga cccagccacg tggccagcgt cagggcgtcg
     5161 tcggcatcgg acgccgatcc ggccggcagg ctccgatgga gctcggtgac gagcgcgtcc
     5221 aggtcgtcga ggtgctgatc gc
//
LOCUS       DBNGSH010000852         3275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4178658, whole genome shotgun sequence.
ACCESSION   DBNGSH010000852 DBNGSH010000000
VERSION     DBNGSH010000852.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4178658"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..607
                     /locus_tag="AC16CY_19575"
     CDS             197..607
                     /locus_tag="AC16CY_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3356060.1"
                     /translation="MPVITRLGATVPLLQVFDMQTSLRFYCAVLGFEVVQQTDHDWWA
                     MIRLGDATLMLNTAYEDNRRPLSPDPKRVRGHNDVSLYFEFPDLDALYAHLRASGCDV
                     KPPATTSYGLRQLNVKDPDGYELCFTAPLETQPG"
     gene            682..1056
                     /locus_tag="AC16CY_19580"
     CDS             682..1056
                     /locus_tag="AC16CY_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3356061.1"
                     /translation="MRTPHPTLTPQELAIMKVVWRREKSTVRDVYEALRETRQVAYTT
                     VMTMMKILEEKGYLKKTSVDRAHLYKPARPRQQVVGAMVRDFLDRVFDGASDSLLLHL
                     AKDNKLTEKQRRIVKQIIEESD"
     gene            1056..2837
                     /locus_tag="AC16CY_19585"
     CDS             1056..2837
                     /locus_tag="AC16CY_19585"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR3356062.1"
                     /translation="MDRAVIADLVAYSAQIACLALVGGLLPSLLRVDAAGVRYAYFRA
                     VLALCLVLPWLQGRQEAGEAVVTTTLSSAVVGGPAASGAQVAAAAPFPWLTALGIVLA
                     GGVAARAIWIAIGLIRLRRLRGAGHQAPACEEHQELQRLIGTCADIRYVAGLGQPVTF
                     GVRRPLVLLPDSLRAQPVLVQRAVLSHELIHVRRRDWAWLLAEEAVRAVFWFHPGVWW
                     LVSRVQLAREEVVDQMAMLATGTRRAYLEALAAFADETPLVPAAAFARRRHLIRRMRL
                     ISREGSMSSRRVVLSSAAMALVVIAGGWYAVGAFPLREAPEAQEALTQAGPLERQAKA
                     ITPENPVPRRIISVDPVYPPEAAIAEVNATVTLMIMINGSGSVGEVRRVGVSPTGLPG
                     TAIDQQAMRTALDALVRAGTDAVRQWQYEVPAEAPIAVRVTFAFGPNIAPRQIAHDAW
                     SVVTVAGGVGAARGRQGGPVQLGAAPPPPPPPPLPPSQNLTASPEWAVGAIRVGGAIK
                     PPAKMKHVNPVYPPIAMSSRVQGVVILEVVIGADGRVAQARVLRSIPLLDQAAIDAVM
                     QWEFTPTLLNGQPVPIIMTTTVQFTLT"
     gene            3096..>3275
                     /locus_tag="AC16CY_19590"
     CDS             3096..>3275
                     /locus_tag="AC16CY_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356063.1"
                     /translation="MRSVCGLIATVVVLAGCGNPETTTAASIPAAAHTPQPYSNLAQM
                     MRGIPFTFANIVFDAQ"
BASE COUNT          540 a         1101 c         1102 g          532 t
ORIGIN      
        1 tgcctcgagt gatcgccatg cctgccatcc tcggtccggc agccttcgac ttcgctcagg
       61 ccaggaaagc cggacaccac gtacgtccac gccgagcggc cagtctagcc cagacactca
      121 cccggcatca tcatctactt ccctgcttcg cggcgggacg tccgcgatag actccgcgtc
      181 tgcctgattc gtcgacatgc cagtcatcac acggctcggt gccacggtac ctctcctgca
      241 ggtgttcgac atgcagacgt cgctcaggtt ctattgcgcc gtcctcgggt tcgaggtcgt
      301 tcagcagacg gaccacgact ggtgggccat gatccgtctt ggcgacgcga cgctgatgtt
      361 gaacaccgcg tacgaagaca atcggcgtcc gctgtcgccc gatccgaaac gcgttcgcgg
      421 ccacaacgac gtgtcgttgt atttcgagtt tccggatctc gacgcgctgt atgcgcacct
      481 gcgggccagc ggctgcgacg tgaagccgcc ggcaacaacg agctacggtc tgcgccaatt
      541 gaacgtgaag gaccccgacg gctacgagct gtgcttcacg gcgccactcg aaacccagcc
      601 aggatgagca gggacgggcc cccggaatcg tggatcttga caagggcgcc cgagtactac
      661 taaatagtta gtactaaata catgagaacc ccgcatccga cgctgacccc gcaggagctc
      721 gccatcatga aagtcgtgtg gcggcgcgag aagtccaccg tccgcgacgt ctacgaggcg
      781 ctccgcgaga cgcgccaggt cgcctacacc accgtgatga cgatgatgaa gatcctggag
      841 gagaaggggt acctgaagaa gaccagcgtc gatcgcgccc atctgtacaa gccagccagg
      901 ccgcggcagc aggtcgtggg cgccatggtc cgggacttcc tggatcgcgt cttcgacggc
      961 gcatcggaca gcctgctcct ccacctcgcg aaggacaaca agctgaccga gaagcagcgg
     1021 cggatcgtca agcagatcat cgaggagagc gactgatgga cagagcggtg atcgcagacc
     1081 tcgtggccta cagcgcgcag atagcctgcc tggcactcgt gggcggcctg ctgccgtcgc
     1141 tgcttcgcgt ggacgccgcg ggcgtccgct acgcgtactt ccgcgccgtt ctggcgctct
     1201 gcctcgtgct gccgtggctg cagggccgcc aggaggcggg cgaggccgtc gtgacgacga
     1261 cgttgtcttc cgccgtcgtg ggtggtcccg cggcttccgg cgcgcaggta gccgctgccg
     1321 ctccgtttcc atggctgacc gcgctcggca tcgtcctggc cggcggcgtc gccgcgcgcg
     1381 cgatctggat cgcgatcggc ttgatccgcc tgcgacgcct gcgcggcgcc ggccatcagg
     1441 cacccgcgtg cgaggagcac caggaactgc agcggttgat cggcacctgc gcggacatcc
     1501 ggtacgtcgc cgggctcggc cagccggtga cgttcggcgt ccggcggccg ctcgtgctgc
     1561 tgccggactc cctgcgcgcg cagccggtgc tcgtgcagcg cgcggtgctc agtcacgaac
     1621 tgattcacgt gcggcggcgc gactgggcat ggctgctcgc cgaagaggcg gtgcgagcgg
     1681 tgttctggtt ccatcccggc gtgtggtggc tcgtctcgcg ggtgcagctc gcgcgggagg
     1741 aagtggtgga ccagatggcg atgctcgcca cgggaacgcg gcgcgcgtac ctcgaggcgc
     1801 tcgcggcatt cgccgacgag acgcctctgg tcccggcggc cgcctttgcc aggcgccggc
     1861 acctgatcag gcgaatgagg ctgatttcga gggagggaag catgtcgtcc agacgagtcg
     1921 tgctgtcgag cgccgcgatg gcgctggtgg taatcgcggg cggctggtac gccgtcggcg
     1981 cgttcccgct gagagaagcg cctgaggcac aagaggcgct cacgcaggcc gggccgctcg
     2041 agagacaggc caaggcgatc acgccggaga acccggtgcc gcgccgcatc atctcggtcg
     2101 atcccgtgta tccgcccgag gccgcgatcg ccgaggtcaa tgcgacggtc acgctgatga
     2161 tcatgatcaa cgggtccggc agcgtgggcg aagtccgcag agtcggcgtg tcgccgaccg
     2221 gcttgccagg gacggccatc gatcagcagg cgatgcggac ggcgctcgac gcgctcgtgc
     2281 gcgcgggcac cgacgccgtc cggcaatggc agtacgaagt gccggccgag gcgccgattg
     2341 ccgtccgcgt gacgtttgcg ttcggcccga atatcgcgcc acggcagatc gcgcacgacg
     2401 cgtggtcggt cgtcacagtg gccggcgggg ttggggcagc ccgtggccgg cagggtgggc
     2461 cggtccaact gggcgctgca cccccgccgc ctcctccgcc gccactgcct ccctcgcaga
     2521 acctcacggc gtcacccgag tgggccgtcg gcgcgattcg tgtgggcggc gccatcaagc
     2581 cgccggcgaa gatgaagcac gtcaaccccg tgtatccacc gatcgccatg tcgtctcgcg
     2641 tgcagggcgt ggtgattctg gaagtcgtga tcggcgctga cggcagagtc gcccaagcgc
     2701 gcgtgctgcg ctcgattcca ctgctcgatc aggcagcaat cgacgccgtc atgcagtggg
     2761 aattcacgcc gacgctgctc aacggccagc cggtgccgat catcatgacg acgaccgtgc
     2821 agttcacgct cacctaactc cttggccctg gtccttggtc cggctacccg gaccaaggac
     2881 ccgccaccac ccgccgccgc ctgaatttct tcgcggcctg cgcgaaaaaa gccgcgcgca
     2941 gagccggtcg tgtgttgaat caacgtccac ggattacgcg tagggccagg aactaacccc
     3001 cacggaccaa ggacctacgt ctggccggat gcttgcaccc gctcgcgcca cgctcgcaaa
     3061 cggcccggtg agcggacgcg cgaggagaga gcgccatgag aagcgtgtgt gggttgatcg
     3121 caaccgtcgt tgtgctggca gggtgcggga atccggagac gacgaccgcg gcgtcgatcc
     3181 cggcggccgc gcacacgccg cagccgtact cgaatctcgc gcagatgatg cgcgggattc
     3241 ccttcacgtt cgccaacatc gtcttcgacg cgcag
//
LOCUS       DBNGSH010000853         2721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2820059, whole genome shotgun sequence.
ACCESSION   DBNGSH010000853 DBNGSH010000000
VERSION     DBNGSH010000853.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2820059"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="AC16CY_19595"
     CDS             complement(<1..1345)
                     /locus_tag="AC16CY_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3356064.1"
                     /translation="MRSALPVEISGEPGIGRTALLRRVAHLSDLATTPHGVVYLNAVG
                     QLTGDLLQSLFDAFYESPSSFKPTPSQLQRLLHDRRAVLLLDDVDLTRDGIEQLLDAV
                     PESSVVIADRQRTLWAEAQSIELGGLPEAESVLLLARELGRAVSEGERPAAKALATSL
                     GGHPIRLLQAGATARRAQVSLNQIVSHVESRGPSALTAQLVMGLGDEDRRVLAALVAV
                     GMALSANAVAAIAQLADPGPSLQRLADRGLVQARRDRYGLVPGVAIPQDITQAAESSS
                     DRVQGFYSEWAAAHARTPDAIASDADTLLRVQADAAAKGESSRVLRIGTALGVVLAVS
                     GRWEAWAAVLDRNLAAARAIGDRAAEGLALHDAGTRALCVGDKAVASRLLKQAIEIRS
                     ANGDRLGAAISRHNASLVSPVPVDPADSLPRPQEIQIDYDTLRSDGIPHAAVPAGS"
     gene            complement(1681..>2721)
                     /locus_tag="AC16CY_19600"
     CDS             complement(1681..>2721)
                     /locus_tag="AC16CY_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356065.1"
                     /translation="GGRGRGMNPSEVRSVPAESKAAKYANPGWKAPRTAWGDPDIEGE
                     YSSDDMRGVPMSRPAAQAARESLTPDEFLQRARGDEANKFSSVNTETFLRNEYGIRTF
                     GYTSLIVDPPDGRQPAVTPAEQKRRAAQPERGTFSNVVLAGFDDFTLYDRCISRGVPG
                     SVNAVLYGNGVRFVQSPTAIVMTYEMIHESRVIHLGSAPPRAPAHGSWTGYSVGRWEG
                     DTLVVETKGFNSRTATSENLKLTEWFTRVDPDMVEYRYRIDDPGVYTAPYTVRMMLTT
                     QPNYFVMEYSCHEGNSAVEMGLKGERAYERSVEEARAKGLPIPERMPRNMDVYSGRAA
                     GPVREIGFETKK"
BASE COUNT          447 a          931 c          901 g          442 t
ORIGIN      
        1 ctgaaccagc gggaactgcg gcgtgtggta tcccgtcgga gcggagggtg tcgtagtcga
       61 tctgaatctc ctgaggtcgc ggcaacgagt ccgccgggtc cacaggcacc ggcgacacca
      121 ggctggcatt gtgccggctg atcgcggctc ctagacgatc gccattcgcg ctgcgaatct
      181 cgatcgcctg cttcaagagg cggctggcga cggccttgtc gccgacacag agtgcgcggg
      241 tccccgcatc gtgcagcgcg agcccttcag ccgcgcgatc cccgatcgcg cgagccgcgg
      301 caaggttgcg atcgaggacc gccgcccagg cctcccagcg tccgctgacg gccagcacga
      361 cgccaagcgc ggtcccgatg cgaagcacgc gagaggattc gccctttgcg gcggcgtccg
      421 cctggacgcg aagaagggtg tccgcgtcag acgcgatcgc gtccggagtt cgtgcgtgag
      481 ccgcggccca ctcggaatag aagccctgca cacgatcgct ggacgattcc gccgcctgag
      541 taatgtcctg cggaatggcg acgccgggga cgagtccata ccggtcccgt cgcgcctgaa
      601 ccagacctcg gtcggccagt ctctgcagac tgggcccggg atcggcaagc tgggcgatcg
      661 ctgccacggc gttcgcagac agcgccatgc cgacggcgac cagcgccgcc agcacccggc
      721 gatcctcgtc cccgagtccc atcacgagct gcgccgtcag cgcggaaggc ccgcggctct
      781 cgacgtgcga cacgatctgg ttgagcgaca cctgcgctcg ccgggccgtc gcgcccgcct
      841 gaagcagccg gatcgggtgt cccccgagcg aggtcgccaa cgccttcgcc gcggggcgct
      901 cgccttccga tacggcgcgt cccagttcac gcgcgaggag aagtacgctc tcggcctcgg
      961 gcagcccgcc caactcaatc gactgtgctt ccgcccacag cgttcgttga cgatcggcga
     1021 tcaccactga cgattccggc accgcgtcga gcaactgctc gatgccatca cgcgtcagat
     1081 cgacgtcatc cagaaggaga acagcccggc ggtcatgcag aagccgctgc agctgcgagg
     1141 gcgtcggctt gaaggacgac ggcgactcgt aaaaggcatc gaagagcgat tgcagaaggt
     1201 cgcctgtcag ctgtccaacg gcattcagat agacgacgcc gtgcggcgtg gttgccagat
     1261 cggatagatg cgccactcga cgcagcaacg ccgtgcgtcc gataccgggc tcgcccgaga
     1321 tctcgacggg gagcgcggag cgcaatcctc tgaccgcagt ttcgagctca gcctcacggt
     1381 caagccagcc gttgatcgga cgtggccgga gaaggatggg accagatcgc tcgcggggct
     1441 gtggctgcga gcccggtgcc gcgaggttca ccaccccgcc gtgaacagac ccgatctgaa
     1501 ggatgtagtt accgaccgca acctggccgg tcacgtcccc ttggatctgc gcctgaatcg
     1561 actcggcaga atcttgtgag ctcacgatgc accgcccacg aaggaagcct ggggatccgg
     1621 gtcagaagta tatagcccgg gtcgacgagg ccggcgcgaa caggtaagct cagccgaaga
     1681 ctacttcttc gtctcgaagc cgatttcccg aaccgggccc gcggcgcgcc cgctgtagac
     1741 gtccatgttg cgcggcatgc gctcgggaat cgggagcccc ttggccctgg cttcctccac
     1801 cgagcgttca taggcacgtt cgcccttcag tcccatctcg acggccgagt ttccttcgtg
     1861 gcacgagtac tccatcacga agtagttcgg ctgggtcgtc agcatcatgc gcaccgtgta
     1921 gggcgccgtg tacacacccg gatcgtcgat gcggtacctg tattcgacca tgtccggatc
     1981 gacgcgcgtg aaccactcgg tcagcttcag gttctcgctg gtggccgtcc gtgagttgaa
     2041 ccccttggtc tcgacgacga gcgtgtcgcc ttcccagcgt cccaccgagt agccggtcca
     2101 cgacccgtgc gccggggcgc ggggcggcgc gctgccgagg tgaatgacgc gcgactcgtg
     2161 aatcatctcg taggtcatca cgatcgcggt cggcgactgc acgaagcgga cgccgttgcc
     2221 gtagagcacg gcgttgaccg atcccggcac gccgcgcgag atgcagcggt cgtagagggt
     2281 gaagtcgtcg aagccggcga gcacgacgtt gctgaacgtg ccccgctcgg gctgcgccgc
     2341 gcgccgcttc tgctcggcgg gcgtcaccgc cggctgccgg ccgtctggcg ggtccacgat
     2401 gagcgaggtg tagccgaacg tacggatgcc gtattcgttg cgcaggaacg tctcggtgtt
     2461 caccgagctg aacttgtttg cctcgtcgcc gcgcgcgcgc tggaggaact cgtcgggcgt
     2521 caacgactcg cgagcggcct gcgcggccgg ccggctcatc ggcacgccgc gcatgtcgtc
     2581 gctcgagtac tcgccctcga tatccggatc gccccacgcc gtgcgcggag ccttccaccc
     2641 gggattggcg tacttggcag ccttcgactc ggccgggacg ctccggacct ccgacgggtt
     2701 catgccgcgg ccgcggccgc c
//
LOCUS       DBNGSH010000854         1852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1971316, whole genome shotgun sequence.
ACCESSION   DBNGSH010000854 DBNGSH010000000
VERSION     DBNGSH010000854.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1971316"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16CY_19605"
     CDS             <1..188
                     /locus_tag="AC16CY_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HIT family hydrolase"
                     /protein_id="HIR3356066.1"
                     /translation="INLGKAAGAGIHEHLHVHLVPRWSGDTNFMTVVGDTRVLPEDLA
                     STAARLAPVFERLARGG"
     gene            278..1252
                     /locus_tag="AC16CY_19610"
     CDS             278..1252
                     /locus_tag="AC16CY_19610"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3356067.1"
                     /translation="MDFNLSDTQLTWQSKARSLGRSLAARASAADVVRAAAAAGVAGP
                     APDRLAAVVAIEALAFESSVAGISLALHGGVAAGLAGLGDARFAAPGRGETVGAIALS
                     ADEVPSESDGRLSGRARWVAPLTSGGVAIVGARGRDGLMAAAVALDGPGITAEPIETA
                     GLSGLVCGHVLFAGAPSIALGATMPFMALVRLLLTAAGLGIGRRALHEALTAARGHTG
                     RGAGGEQTVQGLLADAATELDAATLLTWKAATASELSLADASMAKLAATEATQRAVAR
                     ATQVVGADSFRSDHIVERLAQDVRALELFAGRTEALREAVALDTLPRS"
     gene            complement(1262..>1852)
                     /locus_tag="AC16CY_19615"
     CDS             complement(1262..>1852)
                     /locus_tag="AC16CY_19615"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR3356068.1"
                     /translation="SLNAAYDITWDPPNRHVVKSDALALRARTEDELTANRVGVNVRD
                     EYHLTPRLFVFGQNQYLKDEFKSIDYLIAPTGGVGFKVIDTMATQLGVDGSAGGVWEK
                     NAGLDVNGSGALAVGQKLTQTLTSNTTLTEAFSGLWKTSDFEDSLFTLGIGIAVSMST
                     HTQLKLEALDTFKNKPPNPTIEKNDLALLFSFVYKM"
BASE COUNT          250 a          614 c          670 g          318 t
ORIGIN      
        1 gcatcaacct cggcaaggcg gccggggccg gcatccacga gcatctgcac gtgcacctgg
       61 ttccgcgctg gagcggcgat accaacttca tgacggtcgt cggcgacacg cgcgtgctgc
      121 cggaggacct ggcgtccacg gcggcgcgcc ttgcacccgt tttcgaacgg ctcgcccgcg
      181 gaggctaaag cctccgctct acgacgacgg gtgagccgtg tggtgactat gattcgcgta
      241 gagcgcaggc ttcagcctgc gcggatacgt ctcgcacatg gatttcaatc tctccgacac
      301 ccagctcaca tggcagtcga aggcgcgatc actcgggcgc agcctggcag cacgcgcatc
      361 cgcggcggat gtcgtccgag cggccgccgc cgccggcgtt gcggggcccg cccccgatcg
      421 gctggcggcg gtcgtcgcga tcgaggcgct ggccttcgag tcttccgttg ccggcatttc
      481 gctggcgctg cacggcggtg ttgcggcggg actggccggc ctgggcgatg cccgctttgc
      541 cgcgccaggc cgcggcgaaa ccgtcggcgc gattgcgctc tcggccgacg aagtaccgtc
      601 tgaaagcgac gggcgcctgt cggggcgtgc tcgatgggtg gcgccgctca cgagcggcgg
      661 agtcgcgatc gtcggcgcgc gcggccgcga cggcctcatg gcggccgccg tcgcactcga
      721 cggcccgggg attacggcgg agccgatcga gacagccgga ctctcggggc tggtgtgcgg
      781 ccacgtgctg ttcgccgggg cgccgtcgat cgcgctcggg gcgacgatgc cattcatggc
      841 cctcgtgcgg ttgctgctga cagcggcggg cctgggcata ggacgccgcg ctctgcacga
      901 ggcgctcacc gcggcacggg gtcataccgg gagaggcgcc ggcggcgagc agaccgtgca
      961 gggtctgctt gccgacgccg ccacggagct cgacgcggcg acgttgttga cgtggaaggc
     1021 cgcgaccgcg tccgagctgt cgctcgccga tgcgtcgatg gccaagttgg ccgcgaccga
     1081 ggcgacgcag cgcgccgtgg cgcgggcgac gcaggtcgtg ggcgccgact cgttccgcag
     1141 cgatcacatc gtggagcgtc tggcgcagga cgtgcgcgcc ctcgagctgt tcgcggggcg
     1201 gaccgaggcg ctgcgcgagg cggtcgcgct cgacacgctc ccgcgctcgt gagcgcgccg
     1261 cctacatctt gtagacgaag gaaaacagca gggcgaggtc gtttttctcg atcgtcgggt
     1321 tcggcggctt gttcttgaac gtgtcgagcg cctccagctt cagctgcgtg tgcgttgaca
     1381 tcgagacggc gatgccgatg ccgagcgtga agagggagtc ttcgaagtcc gaggtcttcc
     1441 acaggccgct gaaggcttcg gtgagcgtcg tgttcgacgt gagcgtctgc gtgagcttct
     1501 gtcccacggc cagcgcgccg gagccgttga cgtccaggcc ggcgttcttc tcccacactc
     1561 ctccggcgct tccgtccacc ccgagctgcg tcgccatcgt gtcgatgacc ttgaacccga
     1621 cgccgcccgt gggcgcgatc aggtagtcga tgctcttgaa ttcgtccttc agatactggt
     1681 tctgcccgaa cacgaacagg cgcggcgtga gatggtactc gtcgcgaacg ttgacgccga
     1741 cgcggtttgc ggtcagctcg tcctcggttc gtgccctcag cgcaagcgcg tcggacttga
     1801 ccacgtgcct gttcggcggg tcccaggtga tgtcgtaggc cgcgttgagc ga
//
LOCUS       DBNGSH010000855         2794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_782917, whole genome shotgun sequence.
ACCESSION   DBNGSH010000855 DBNGSH010000000
VERSION     DBNGSH010000855.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_782917"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="AC16CY_19620"
     CDS             complement(<1..942)
                     /locus_tag="AC16CY_19620"
                     /inference="COORDINATES: protein motif:HMM:NF014312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3356069.1"
                     /translation="MNALIRCGVALVLVFTSTAAFSQVRIVQTNSQGDNIHLIDPVTQ
                     RIVGEVKGIPINHGAAASPDGRQLYFSSEAEQTLHVVDAKTLQTTKKIPLTGRPNNIS
                     ISRDGRRVYVGIVSQPGAVDVIDTASLDKVKSIPTKGGIHNVYVTPDGRHVVAGSIAG
                     RVMTVIDQKTEEPIWTLFEEGVRPMAFETNADGSTKRIYVQLSNLHGFAIVDFQQRKE
                     VARVELPNDVPAEKIDKGPFNASPSHGLGVAPDGKSLWVTSRPNARVYTYTLPELKLL
                     PDPVDLGGRPDWVTFTPDSKLIYITTENTDLVHAIDVT"
     gene            complement(1019..1405)
                     /locus_tag="AC16CY_19625"
     CDS             complement(1019..1405)
                     /locus_tag="AC16CY_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3356070.1"
                     /translation="MIPERYAPTTYALFRIVFGFLFMCFGTQKMFGWFGGPAAELMTL
                     QGAAGVIETVCGALIMLGFFTKQAAFLASGEMAVAYFYIHWRLMGLVPLVNMGERSTM
                     FCFAFLFIATQGAGLWSLDGLRGRKK"
     gene            complement(1432..2622)
                     /locus_tag="AC16CY_19630"
     CDS             complement(1432..2622)
                     /locus_tag="AC16CY_19630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3356071.1"
                     /translation="MIDLRERSYAVACAAMFVFGMILGLPGTVLGQPETVAQFGLTMA
                     DRGVLISTLYFGLLFGSLASGPFVDAIGHRAAMAVSSALAAVCLPLFAWAGNAAFAAI
                     VLGTVGFASASINTASNALASELFSEERARRMNGLGVLFGFGGLAMPAATVLASDLVT
                     WRGVVVVGGVLSAVVALTAALMPMGGVRPAHASARLSPVAAFRHFARQPGFAWFCLLV
                     MLAGGNEASMAGWTSTFLIASGVSASAATIILSSHWLGVILSRVIFSRYVDRAKTAAI
                     ERSAVAGALLVLIFVISLEPVVLAAGPFAIGMAIALVMPTSLALAGERVAGNPGTLFG
                     LLLTLAQVGGMVLPASIGLVAERAGVRPGLAILVASCAAIVLVVRHISASVPGAYQAG
                     PKGG"
BASE COUNT          526 a          919 c          920 g          429 t
ORIGIN      
        1 cgtgacgtcg atcgcgtgca cgaggtcggt gttctccgtc gtgatgtaga tcagcttact
       61 gtccggcgtg aaggtcaccc agtcgggccg tccgccgaga tcgaccgggt ccggcagcag
      121 cttcagctcc ggaagcgtgt aggtgtagac gcgagcgttc ggcctgctcg tcacccacag
      181 cgacttcccg tccggcgcga ctccaagccc gtgcgaggga gacgcgttga aagggccctt
      241 gtcgatcttc tccgcgggga cgtcgttcgg cagctcgacg cgcgcaactt ccttccgctg
      301 ctgaaaatcg acgatcgcga agccgtggag gttcgacagc tgcacgtaga tccgcttcgt
      361 cgagccgtcg gcgttcgtct cgaacgccat cgggcgcacg ccttcctcga acagcgtcca
      421 gatcggctcc tcggtcttct ggtcgatcac ggtcatgacc ctgcccgcga tcgagccggc
      481 cacgacgtgc cttccatccg gcgtcacgta gacgttgtgg atgccgccct tggtcgggat
      541 gctcttgacc ttgtcgagcg atgccgtgtc gatgacatcc acggcgccgg gctgggagac
      601 gatgccgacg tacacgcgcc gtccgtcgcg gctgatcgag atgttgttcg ggcggcccgt
      661 caacggaatc ttcttcgtcg tctgcagggt tttcgcgtca acgacgtgga gcgtctgctc
      721 ggcctcgctg ctgaagtaga gctgcctgcc atcgggcgac gccgcggcgc cgtggttgat
      781 cggaatgcct ttgacttcgc cgacgatccg ttgcgtcaca ggatcgatca ggtggatgtt
      841 gtccccctgc gagttggtct ggacgatccg gacttgtgag aaggcggcgg tcgaagtgaa
      901 caccaggacc agcgccacgc cgcagcggat gagtgcgttc atgatggctc cttcgtacgc
      961 cgtggagagt agcacggctc cgggctctgg ccgagagccc ggagactacg gttagagcct
     1021 acttcttcct ccctctgagt ccgtcaagac tccacaggcc tgcgccctgg gtcgcaatga
     1081 acagaaacgc aaagcagaac atcgtggacc gctctcccat gttcacgagc gggacgaggc
     1141 ccatcaagcg ccagtggatg tagaagtagg ccacagccat ctcgccgctc gccaggaatg
     1201 ccgcctgctt ggtgaagaag ccgagcatga tgagcgcacc gcacaccgtt tcgatgacgc
     1261 cagcggcgcc ctgcagcgtc atcagctcgg ccgccggtcc gccgaaccag ccgaacatct
     1321 tctgcgttcc gaagcacatg aacaggaaac cgaacacgat acggaacagt gcgtacgtcg
     1381 taggcgcgta gcgttctgga atcatcatct ccctccgctg ggtccgggat tctatccgcc
     1441 tttgggtccg gcctggtaag cgcccggcac cgacgcggaa atgtgccgga cgacgagcac
     1501 aattgccgca cagctcgcga ccagaatggc gaggccgggc cgtacgccgg cacgctcggc
     1561 caccagcccg atcgacgcgg gaaggaccat gccgccgacc tgcgccagcg tgagcaggag
     1621 gccaaacagc gtcccggggt tgccggccac gcgctcgccg gcgagcgcga gcgaggtggg
     1681 catcacgagc gcgatcgcca tgccgatcgc gaacgggccc gcggcgagca cgacaggctc
     1741 caacgagatg acgaatatca gcaccagcag ggctccagcg acggcgctcc gctcgatggc
     1801 agcagtcttt gcccggtcga cgtaacgaga aaagatcacg cgcgacagga tcactcccag
     1861 ccagtgcgac gagaggatga tggtggctgc cgacgcgctc acgccggacg cgatcaggaa
     1921 cgtcgacgtc catccggcca tcgacgcttc gttgccgccg gccagcatga ccagcagaca
     1981 gaaccaggca aagcccggct gccgcgcgaa gtgacgaaac gcagcgaccg gtgacaggcg
     2041 cgccgacgcg tgcgccggtc ggacgccgcc catcggcatg agggcggctg tcagggcgac
     2101 gaccgccgag agcaccccgc cgacgacgac cactccgcgc cacgtcacga gatcggaggc
     2161 gagcaccgtc gccgccggca tggccaggcc gccgaatccg aacagcacgc cgaggccgtt
     2221 catacggcgg gcgcgctcct cggaaaacag ctccgacgca agcgcgttcg acgccgtgtt
     2281 gatgcttgcc gacgcgaatc cgaccgtacc gagcacgatc gccgcgaagg ccgcgttgcc
     2341 ggcccatgcg aagagcggca ggcagacggc agccagcgcc gacgagacgg ccatcgcggc
     2401 gcgatgaccg atggcgtcga cgaaaggacc cgacgccagg ctgccgaaca gcaggccgaa
     2461 gtagagcgtc gagatgagca cgccgcgatc ggccatggtg aggccgaact gcgccaccgt
     2521 ttccggctga cccagtacgg ttccaggcag accgaggatc atgccgaaga cgaacatcgc
     2581 ggcgcacgcg accgcgtacg agcgttcgcg gagatcgatc actgaggcgg agtcgcggtc
     2641 ttacgggcag ggttgaagaa cggcgtacgg acgacggttg cgcggacgaa gtggcgaacg
     2701 ccttcgaccg acatctcgat cctgagtcgc gcgccttcga ccgattgccg cacatcgacg
     2761 caggcgatgg cgatgaggcg cttgagcgtc ggcg
//
LOCUS       DBNGSH010000856         6339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2480806, whole genome shotgun sequence.
ACCESSION   DBNGSH010000856 DBNGSH010000000
VERSION     DBNGSH010000856.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2480806"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="AC16CY_19635"
     CDS             complement(<1..231)
                     /locus_tag="AC16CY_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003232058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3356072.1"
                     /translation="MSGRVQLAPSILAADFAALGRDIAAVERGGADLIHVDVMDGHFV
                     PNITIGPPVVRAIKRVATRPLDVHLMIEHPDRY"
     gene            complement(228..950)
                     /locus_tag="AC16CY_19640"
     CDS             complement(228..950)
                     /locus_tag="AC16CY_19640"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR3356073.1"
                     /translation="MRFGSRVWNLGKSFVLVGALGATALLSFGISMRIALWARDVTVP
                     SLIGRSVNEATAELAGLGLGLRVDEKPRPDDKVEAGRVMQQDPEGGVVSRRQRTVRVW
                     VSSGPKRTIVPALVGQGERAAQMRVQQDGVAIASVSEFRSADYPAEVVAAQNPAPSAR
                     SPHVSLLVNRGEMATTYVMPDVIGVDGDRAAEALRRQGFRVSIVGTQPYPGVPPGTVV
                     SQQPAGGFRVGAADAISLEVSR"
     gene            complement(979..2328)
                     /gene="rsmB"
                     /locus_tag="AC16CY_19645"
     CDS             complement(979..2328)
                     /gene="rsmB"
                     /locus_tag="AC16CY_19645"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR3356074.1"
                     /translation="MTAPARTAAFRALTAVASESRDLPSALADSRARLADERDRSLAA
                     AIVHGTLRWQRACDHLVEHASRRRLDALDTDVLVILRLSLFQLLHLDRVPAAAVVDDA
                     VDLTRMAGRKSAAGFVNAVLRSTLRRRHALPLPPRPDDLSDREAVIRYLGITCSHPDW
                     LVSRWLDRFGFAKAERWVQFNNASAPLTLCVNRMRSSRDDVRAALAADGILTEDVSFA
                     PHGLRVVEGNPLRRPSLGRFLVQDEASQLVPLAVDARPGERVLDVCAAPGGKTVLMSG
                     DMRDTGVLAACDVRPRRVALLRRTIGDAGSRHAHAVHVAAGGHLPFGTVFDRVLVDAP
                     CSGLGTVRRDPDIKWRRREDDLAGLAGRQIALLRRAGRTVGPRGRLVYATCSSEPEEN
                     EMVVDAFLSAEPAFRLIDLRVSGPPALAPVLDERGVLRTLPFAHGLEAFFAAAMERH"
     gene            complement(2325..3329)
                     /gene="fmt"
                     /locus_tag="AC16CY_19650"
     CDS             complement(2325..3329)
                     /gene="fmt"
                     /locus_tag="AC16CY_19650"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3356075.1"
                     /translation="MAVSVESHPRTFAPPHLRTSGGLRIVFFGTPEFAVPSLQRLISS
                     RHYVVGVVTQPDRPRGRGQRVSDSPVKTTALSGELPVLQPDRLREPGVADTIRTWAPD
                     LGVVVAYGKLIPESLLAIPRLGMINVHGSLLPKYRGAAPVHRAVIDGAAETGVTIIRV
                     AALLDSGNMFAKAKRPIGPDETSDVVERDLAELGASLLMTVVEDLAAGRAAEEPQDDM
                     LSTYAARLTKDEGLVDWALPAAYIHNRVRGLYPWPHAYTYLAGDRVIILRTRVDSAPT
                     TEPPGTVVEASNDRLRVATGHSGLIAIEDLQLEGRRAMSSREFLAGHSIRPGARFGSQ
                     "
     gene            complement(3340..3849)
                     /gene="def"
                     /locus_tag="AC16CY_19655"
     CDS             complement(3340..3849)
                     /gene="def"
                     /locus_tag="AC16CY_19655"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3356076.1"
                     /translation="MLRPIVRYGADVLHRPAAPVAAITPDIQSIVDDMIQTMYAAPGI
                     GLAAPQIGVGVRIFVCDISNGRNAADLMTFVNPEFVERDGMQLEEEGCLSVPGFNATV
                     ARPVRALLKGMDRDGHERTVEATGLLARCFQHEMDHLEGTVFVDRLRGLQKDLIVRKI
                     KKLSRAGKW"
     gene            complement(3861..5081)
                     /gene="aroC"
                     /locus_tag="AC16CY_19660"
     CDS             complement(3861..5081)
                     /gene="aroC"
                     /locus_tag="AC16CY_19660"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3356077.1"
                     /translation="MLRFLTAGESHGQTLVMTLDGLPAGLDLDIDALNVQLRRRQGGY
                     GRGRRMEIESDRAEIVGGVRRGRTTGAPIALLIRNRDWVNWQQTMHVEREMPEGASGT
                     RRAEVTRPRPGHADLAGAIKYGHADIRDVLERASARETAARVAAGSLARQLLGRFGIG
                     VTSHVSAIGGIALPSARMVSFEEARAIPDDSPLRCVDAGLQQRMIAAIDAAREAGDTL
                     GGAFEVIATGLPPGLGSYVQWDRKLDGRLAQAMMCIHAIKAVGIGIGPDVAFRPGSAV
                     HDEILPPAGDGGPLVRPSNNAGGLEGGVTNGEDVRVTGFMKPIATLMQPKRSVDLTTL
                     QDAPAAIERSDVCAVPAAAVVGEAMVSFVLADAFLEKTGGDSIEEVERHLAATAEALE
                     RRFRAARASATPAR"
     gene            complement(5171..6088)
                     /locus_tag="AC16CY_19665"
     CDS             complement(5171..6088)
                     /locus_tag="AC16CY_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356078.1"
                     /translation="MKLFTPKKHEPPRAGTDVVATLAEITQRLEKLERVITDLKQNVD
                     LLPAVVRKLYLDGVELTPPYDLIAERFSYLSQNDEDGLILAIFKRIGTTDRRFVEIGC
                     GMNGGNSGFLAQECGWSGLMMDNRPDAIEKVRTRFTGHQVTVRLARVSRENVDALLAE
                     HGMTGELDLLCIDIDGNDYWVWDAITACRPRVVSIEYNWLFGASRPVTIPYDESFDLA
                     TVGVRAYRGASLAALVHLATRKGYRLVATERVNAFFLRTDVGPEWPALDPARGYRAPM
                     NIAHSRDVFTKIRNAGLPLLNVVTGESET"
BASE COUNT         1001 a         2151 c         2189 g          998 t
ORIGIN      
        1 gtaccgatcg ggatgctcga tcatcaggtg cacgtcgagg ggccgcgtcg cgactcgctt
       61 gatggcccgc acaaccgggg ggccgatcgt gatgttcggg acgaaatgcc cgtccatgac
      121 gtccacatga atcaggtccg cgccgccgcg ctcgaccgcg gcgatgtcac gtccaagagc
      181 ggcgaagtca gccgcgagta tggacggagc gagctgaacg cgaccgctca ccggctcacc
      241 tcgagcgaga tcgcgtcggc cgcacccacg cggaaaccgc ccgccggttg ctggctcacg
      301 acagtgccgg gagggacccc gggatacggc tgcgtgccga cgatggagac gcggaaccct
      361 tgccggcgca gcgcttccgc ggcgcggtcg ccgtcgacgc cgatgacgtc cggcatgaca
      421 taggtcgtcg ccatctcccc tcggttgacg agcagcgaca cgtgcggcga tcgtgcgctg
      481 ggcgccgggt tctgggcggc cacgacctcg gcggggtagt cggccgagcg gaactccgag
      541 accgaggcga tggcgacacc gtcctgctgc acgcgcatct gtgcggcgcg ctcaccctgg
      601 cccacgagcg ccggaacgat cgtcctcttc ggccccgaac tgacccagac gcgcaccgta
      661 cgttggcgcc tgctgacgac gccgccttcc ggatcctgtt gcatgacgcg tccggcttcg
      721 accttgtcgt caggacgcgg cttctcgtcc acgcggagtc ccaggccgag gccggccagt
      781 tcggcggtgg cttcgttgac gctgcggccg atcagggagg gtaccgtgac gtctctggcc
      841 cacagcgcga ttcgcatcga gatgccgaat gaaaggaggg cggtggcgcc cagggcgcca
      901 acaaggacga aggacttgcc caggttccac acgcgggatc cgaagcgcat cgacgggaac
      961 attgtacctt gtccgtgttc agtgtctttc catcgccgcg gcgaagaacg cctccaggcc
     1021 gtgcgcgaag ggcagcgtgc gaagcacgcc tcgctcgtcg aggaccggcg cgagcgccgg
     1081 cgggccgctg acgcggaggt cgatcaatct gaacgcgggt tccgccgaga ggaatgcgtc
     1141 caccaccatc tcgttctcct caggctcgct ggaacaggtg gcgtacacga gcctgccccg
     1201 cggaccgacg gtgcggcccg cgcgtcggag aagtgcgatc tgacgcccgg ccagcccggc
     1261 cagatcgtct tcccgccgtc gccatttgat atcgggatcg cgccgcaccg taccgaggcc
     1321 cgaacatggc gcgtccacga gtacgcgatc gaagaccgta ccgaacggca gatggccgcc
     1381 ggcggcgacg tgcaccgcgt gcgcgtggcg gcttccggca tcgccgatcg tgcggcggag
     1441 cagcgccacg cgtcgcggac gaacatcgca cgcggccagc acgccggtgt cgcgcatgtc
     1501 tccgctcatg agaacggtct tgccgccggg cgcggcgcac acgtccagta cccgctcgcc
     1561 cggccgggca tcaacagcga gcggaacgag ttgtgacgcc tcgtcctgaa cgaggaatcg
     1621 gccgaggctc gggcgccgaa gcgggttgcc ttcgacgacg cgcagtccgt gcggcgcgaa
     1681 cgacacgtct tcggtcagga tgccgtctgc agcaagggcg gcgcgcacgt cgtcacggct
     1741 cgagcgcatc cggttcacgc agagcgtcaa aggcgccgaa gcgttgttga actggaccca
     1801 gcgctccgct ttggcaaacc cgaaccggtc gagccagcgc gagacgagcc agtccgggtg
     1861 cgagcacgtg atgccgaggt accggatgac cgcttcccgg tccgacaggt cgtccggtct
     1921 tggaggcagc ggcagcgcat gccgccttcg cagcgtcgag cggaggacgg cgttgacgaa
     1981 tccggccgcg ctcttccgtc cggccatccg cgtcaggtcc accgcatcgt ccacgacagc
     2041 ggcggcgggc acgcgatcga ggtgaagcag ttgaaagagg ctcagcctca ggatcacgag
     2101 gacgtcggtg tccagggcat cgagacgcct gcgcgacgcg tgctcgacga ggtgatcgca
     2161 ggctcgctgc cagcgaagcg tgccgtggac gattgcggca gccagcgatc gatcgcgttc
     2221 gtcggcgaga cgggcgcggc tgtcggccag ggccgacggc aggtcgcggg actccgaggc
     2281 gacggcggtc agcgcgcgga acgcggccgt gcgcgccgga gccgtcattg gctgccgaac
     2341 cgcgctcccg gccgtatcga gtgacccgcc aggaactcgc ggctgctcat cgcccgccgc
     2401 ccttcgagct gcaggtcttc gatggcaatc agtcctgaat gcccggtcgc cacgcgcagc
     2461 ctgtcgttgg acgcctccac gacggttccc ggcggctccg tggtcggcgc ggagtcgacg
     2521 cgggttctca agatgatcac gcggtcgccg gcgagataag tgtaggcgtg gggccacggg
     2581 tagaggccgc gaacccggtt gtggatgtag gcggcaggca gtgcccagtc cacgaggccc
     2641 tcgtccttgg tgagcctcgc ggcgtaggtg gacagcatgt cgtcctgcgg ctcttctgcg
     2701 gccctgccgg ccgccagatc ctcgaccacc gtcatgagca gcgacgctcc cagttcggcg
     2761 aggtcgcgtt cgacgacgtc gctcgtctcg tcgggaccaa tggggcgctt cgctttcgcg
     2821 aacatgttgc ccgagtcgag caacgcggca acccggatga tggtgacgcc ggtttcggcc
     2881 gcgccgtcga tcacggccct gtgcaccgga gccgcgcctc gatacttcgg cagcagcgac
     2941 ccgtgaacgt tgatcatgcc gagcctcggt atcgcgagaa gagactccgg gatcagcttg
     3001 ccgtaggcaa ccacgacgcc gagatccgga gcccatgtcc ggatggtgtc ggcgacgccg
     3061 ggctcgcgca ggcgatcggg ctggaggacc ggcagctcgc ctgacagcgc ggtcgtcttg
     3121 accggggagt cgctgacccg ctggccgcgc ccacgcggcc ggtccggctg cgtgacgacg
     3181 ccgaccacgt agtgccgcga cgagatgagg cgctgcagcg acgggacggc gaactccggc
     3241 gtgccgaaga agacgattcg cagaccaccg gaggtgcgaa ggtgcggggg tgcgaaggtg
     3301 cgagggtgcg attcgacgct gactgccatt cgtcgtgaac taccatttcc cggcgcgcga
     3361 gagcttcttg atcttccgga cgatcaggtc tttctgcagg ccgcggagcc gatcgacgaa
     3421 gacggtcccc tcgaggtgat ccatctcgtg ctggaagcag cgcgccagca ggccggtcgc
     3481 ctcgaccgtg cgttcgtggc cgtcgcgatc catccccttc agcagcgccc gaaccgggcg
     3541 cgcgacggtg gcgttgaatc ccggcacgct gagacagccc tcttcctcga gctgcatgcc
     3601 gtcccgctcg acgaactcgg ggttgacgaa cgtcatcaga tcggcggcgt tccttccgtt
     3661 ggagatgtcg cagacgaaga ttctgacgcc gacgccaatc tgaggcgccg cgagcccgat
     3721 tcccggcgcc gcgtacatcg tttgaatcat gtcgtccacg atcgactgga tgtccggcgt
     3781 gatagccgcc acgggcgccg ccggcctgtg caggacgtcg gctccgtagc ggacaatcgg
     3841 acgcagcatc ggcccggcga tcagcgcgcc ggcgtcgccg aggctcgcgc ggcgcggaaa
     3901 cggcgttcga gggcctctgc ggtcgcggcc aggtgtcgct cgacttcctc gatggagtcg
     3961 cccccggttt tctccaggaa ggcatcggcg aggacgaacg agaccatcgc ttcgcccacg
     4021 acggcggccg ccgggacggc gcagacgtcg ctgcgctcga ttgcggcagg cgcatcctgc
     4081 agcgttgtca gatcgacgga tcgcttgggc tgcatcagcg tggcgatcgg cttcatgaag
     4141 ccggtcacgc ggacatcctc gccgttggtc acgccgcctt cgagcccgcc agcgttgttc
     4201 gacgggcgca ccagaggccc tccgtccccg gccggcggca ggatctcgtc gtgcacggcc
     4261 gatcccggcc gaaacgccac gtccggtccg atgccgatgc cgaccgcctt gatggcgtgg
     4321 atgcacatca tcgcctgggc gaggcggccg tccagcttcc ggtcccactg cacgtacgaa
     4381 cccaggccgg gcgggaggcc cgtggcgatc acctcgaagg cgccgcccag cgtatcgccc
     4441 gcctcgcggg cggcgtcgat ggccgcgatc atccgttgct gaaggcccgc gtcaacgcac
     4501 cggagaggag agtcgtccgg gatggcgcgc gcttcctcga acgacaccat ccgggccgag
     4561 ggaagcgcga tcccgccgat ggccgagacg tggctcgtga cgccgatgcc gaagcgcccc
     4621 agaagctggc gggccaggga tccggcggcc acgcgtgcgg ccgtctcgcg ggcactcgcg
     4681 cgctcgagca cgtcgcggat gtcggcatgg ccgtacttga tcgcgccggc gagatcggcg
     4741 tgacccggcc gcggacgggt gacctcggcg cgcctggtgc cgctcgcacc ctctggcatc
     4801 tcgcgctcca cgtgcatcgt ctgctgccag ttcacccagt cgcggttccg gatgagcagc
     4861 gcgatcggag cgcccgtcgt gcggccgcgg cgaacgccgc cgacgatctc ggcgcggtcg
     4921 ctttcgatct ccatccgccg gccgcggccg tagcctccct gccgccgccg aagctgcacg
     4981 ttgagcgcgt cgatgtcgag gtcgaggccc gccggcaggc cgtcgagcgt catgacgagc
     5041 gtctgcccgt gcgactctcc ggcggtgaga aatcgcagca tttccgctca attctatacg
     5101 aagacctcgc gcgcttccgg gtctcgtgga ccgtatcaac tggaagagga ccctagtctc
     5161 tacgcgatcg tcacgtctcg gattctccgg tgacgacgtt caggaggggc aggccggcgt
     5221 tccggatctt ggtgaacacg tccctggagt gtgcgatgtt catcggcgcc cggtacccgc
     5281 gtgcgggatc gagggcgggc cactccggcc cgacgtcggt ccgcaggaag aaggcgttca
     5341 cgcgttcggt cgccaccagc cggtaccctt tccgggtcgc gaggtgcacg agcgccgcca
     5401 gcgatgcacc ccggtacgcc cgcacgccga cggtcgccag atcgaagctc tcgtcgtagg
     5461 gaatcgtgac cggccgcgag gcgccgaaca gccagttgta ttcgatggac acgacgcgcg
     5521 ggcggcaggc cgtgatcgcg tcccagaccc agtagtcgtt gccgtcgatg tcgatgcaca
     5581 gcaggtcgag ctcgcccgtc atcccgtgct cggcgagcaa cgcatcgacg ttctcgcgcg
     5641 acacacgtgc cagccgtacg gtcacctgat ggccggtgaa gcgggttctg accttctcga
     5701 tcgcgtcggg ccggttgtcc atcatcaacc cgctccagcc gcactcctgg gccaggaagc
     5761 ccgagttgcc gccgttcatc ccgcatccga tctcgacgaa ccggcgatcc gtcgtgccga
     5821 tccgcttgaa gatcgccagg atcaggccgt cttcgtcgtt ctgagagagg tagctgaagc
     5881 gctcggcgat gagatcgtag ggcggagtca gctcgacgcc atcgaggtac agcttacgga
     5941 cgacggccgg cagcaggtcc acgttctgct tcagatcggt gatcaccctc tcgagttttt
     6001 caaggcgctg ggtgatttcg gcgagcgtgg cgacgacgtc ggttccggct cgcggcggct
     6061 cgtgcttctt cggtgtgaag agtttcacgt cagcacgctc ggcgcatgcc tgcggtgaga
     6121 aatcgcagca tccgcgctca attctatacg aagaaactgc gacctttcgg cgcgtccggc
     6181 tcgattccct ctggaaaccg gcgtgggcac gccgttgcaa gggcgagggc atgaccagat
     6241 tgctgcacga tcgctactac gcctacgcgg acgagcacgc gtgggatctc gcgacaggag
     6301 agagcgtccg gatcggcgag ctcgcggcgg cgaccgcgc
//
LOCUS       DBNGSH010000857         4394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2312443, whole genome shotgun sequence.
ACCESSION   DBNGSH010000857 DBNGSH010000000
VERSION     DBNGSH010000857.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2312443"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="AC16CY_19670"
     CDS             complement(<1..834)
                     /locus_tag="AC16CY_19670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356079.1"
                     /translation="MRLLDWLLRRRREDDLDDEIRAHLAMATRDRIESGEPADSARFA
                     ALKEFGNLTLTHEETRGTWGGRWRSWVIDLMHDVRYAGRVLRNSPGYALVVIVVLAVG
                     LAANVAVFSLFKAIFIKPLTGVHESASLAVVVGRTSAGRIIPLSHLDFRDLQARNTTF
                     ASLAGVTQNRYSLGLGTRGETIWVESVTGNFFDVLGVRAQRGRTLLPTDDEAPGRHPV
                     AVISDAMWQRVFGADPDIVGRTIQIAGYPFEVVGVAAPDFRGSIVSMVTDVFVPVMMH
                     AQ"
     gene            complement(839..1195)
                     /locus_tag="AC16CY_19675"
     CDS             complement(839..1195)
                     /locus_tag="AC16CY_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3356080.1"
                     /translation="MEPKTPATRLDLLQGTLDMLILQTLQWGPQHGYAITQMIRSRSS
                     ETLRVETGSLYPALHRLEKQGWVKSGWDVTEANQRAKYYRLTAAGKKQLLRERDRWSQ
                     LVAAIGGVLDPDPAKG"
     gene            complement(1354..3531)
                     /gene="katG"
                     /locus_tag="AC16CY_19680"
     CDS             complement(1354..3531)
                     /gene="katG"
                     /locus_tag="AC16CY_19680"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HIR3356081.1"
                     /translation="MSNESKCPFSGKAHSGTSNREWWPKQLHLKALHQNPPAGDPMGK
                     DFNYAEEFKTVDLDALKKDIEKVMTTSQDWWPADYGHYGPLFIRMAWHSAGTYRIHDG
                     RGGAGSGTLRFAPLGSWPDNANLDKARRLLWPVKQKYGRKISWADLMVFTGNCALESM
                     GFKTFGFAGGREDVWEPEQDVYWGSERTWLGDERYSGDRQLENPLAAVQMGLIYVNPE
                     GPNANPDPIAAARDIRETFRRMAMNDEETVALIAGGHTFGKTHGAAPATNVGPEPEGA
                     PLEMQGLGWKNSYRTGRGADAITSGIEVIWTSTPTKWSNNFFENLFGYEWELTKSPAG
                     ANQWIAKGGAGAGTIPDAHDPSRRHAPTMLTTDLSLRLDPAYEKISRRFYEHPDQFAD
                     AFARAWFKLTHRDMGPTSRYLGKLVPREPQLWQDPVPPVAHELIGAADIAALKAKVLA
                     SGLSVSQLVSTAWASAATFRGTDKRGGANGARIRLAPQKDWEVNNPPELAKVLKTLEG
                     IQKAFNESGGKKKVSLADLIVLGGCAAVEQAARNAGHDVQVPFRPGRTDASQEQTDVE
                     SFAVLEPTADGFRNYLGKGQELPAEHLLIERALMLTLSAPEMTVLVGGMRALGANARP
                     SNVGVFTARPGTLTNDFFVNLLDMDTEWTPTSGAGETFAGRARGRSEVKWTGSRVDLI
                     FGSNSELRALAEAYACDDAKQKFVHDFVAAWNKVMNLDRFDLA"
     gene            complement(3568..4095)
                     /locus_tag="AC16CY_19685"
     CDS             complement(3568..4095)
                     /locus_tag="AC16CY_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019931753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3356082.1"
                     /translation="MASDPADLLRQRGIQVTAQRLAVLRAVAGQPHAAADAVAESVRA
                     EIGAISLQSVYDCLGVLVAEGLVRRIQPSGSPARFETRVGDNHHHLICRVCGRMVDVD
                     CAVGSAPCLAAANEGGYEIDEAEVVYWGRCPDCQVRSRKSSRAHQRVRLRADTASPAH
                     SSRRPRQRATRKRPD"
BASE COUNT          683 a         1482 c         1446 g          783 t
ORIGIN      
        1 ctgcgcgtgc atcatcacgg gaacgaacac gtcggtgacc atgctgacga tcgagccgcg
       61 gaagtccggc gcggcgacgc ccaccacctc gaacggatag ccggcgatct gaatcgtcct
      121 gccgacgatg tccgggtccg cgccgaacac gcgctgccac atcgcgtccg agatcaccgc
      181 caccggatgc cgacccggcg cctcgtcgtc ggttggcagc agcgtgcgcc cgcgctgcgc
      241 gcggacgccg agcacgtcga agaaattgcc tgtgaccgat tccacccaga tcgtctcgcc
      301 gcgcgtcccc aggccgaggc tgtagcggtt ctgcgtcact ccggccaggc tcgcaaaggt
      361 cgtgttgcgc gcctgcagat cgcggaagtc gaggtgcgac agcggaatga tgcgtccggc
      421 cgaggtgcgc ccgaccacca cagcgagact ggcggactcg tggacgcctg tcagcggctt
      481 gatgaagatg gccttgaaca ggctgaagac cgccacgttg gcggcaagcc ccaccgccag
      541 cacgacgatg acgacgagcg cgtaccccgg gctgttgcgg agcacgcgcc cggcgtagcg
      601 gacgtcgtgc atcaggtcga tcacccagct tcgccagcgg ccgccccagg tgccgcgcgt
      661 ctcctcgtgc gtcagggtga ggttcccgaa ctccttcagc gccgcgaagc gggcgctgtc
      721 ggccggctcg ccgctttcga tgcggtcgcg agtcgccatc gccagatggg cgcggatctc
      781 gtcatccagg tcgtcttccc gccgccgccg cagaagccag tccaggagtc tcatcgcgtc
      841 atcccttcgc cggatccgga tcgagcacgc cgccgatggc ggcgaccagc tgcgaccacc
      901 ggtcccgctc ccgcagcagc tgcttcttcc ccgccgcggt cagccggtag tacttggcgc
      961 gctggttcgc ctccgtgacg tcccagccgg atttgaccca tccctgcttc tccagccggt
     1021 gcagcgcggg atagagcgac ccggtctcca cgcgcagcgt ctccgacgat cgggagcgga
     1081 tcatctgggt gatcgcgtag ccgtgctgcg gcccccactg gagcgtctgg aggatcaaca
     1141 tgtcgagcgt gccctgcaag aggtcgagac gggtggcggg ggtctttggt tccataggct
     1201 gtagtcagcc tacagccatg cctgtagttt gtctacatcc ctgaggtccg gctgccttcg
     1261 acttcgctca ggccaggaaa gccggacact acgtacaaga aaagaggcac gaagaaccgg
     1321 atctccgtgc ctcggtgcct cggtgtgtcg tgatcaggcg aggtcgaacc ggtcgaggtt
     1381 catgaccttg ttccaggcgg ccacgaagtc gtgcacgaac ttctgcttcg cgtcgtcgca
     1441 cgcgtaggcc tccgcgaggg cacgcagctc tgaattcgac ccgaagatga ggtccacgcg
     1501 gctgccggtc cacttgacct cggacctgcc gcgcgcgcgg cccgcgaagg tctccccggc
     1561 gccggacgtc ggcgtccact ccgtgtccat gtcgaggagg ttcacgaaga agtcgttggt
     1621 gagcgtcccg ggccgcgcgg tgaacacgcc gacgttcgac ggccgggcgt tcgcgccgag
     1681 ggcacgcatg ccgcccacga gcaccgtcat ctcgggcgcg ctcagcgtca gcatgagcgc
     1741 ccgctcgatc agcagatgct cggccggcag ctcctgccct ttaccgagat agttgcggaa
     1801 gccgtcggcg gtcggctcga gcacggcgaa cgattcgacg tcggtctgct cctgcgacgc
     1861 atccgtgcgt ccgggcctga agggcacctg cacgtcgtgt ccggcgttcc tggcggcctg
     1921 ctcgacggcg gcacacccgc ccagcacgat cagatcggca agcgacacct tcttcttccc
     1981 gccggactcg ttgaacgcct tctggatccc ttcgagggtc ttcagcactt tcgccaactc
     2041 gggtggattg ttgacctccc agtccttctg cggcgcgagg cggatacgag cgccgttggc
     2101 gccgccgcgc ttgtcggtgc cgcggaaggt cgccgcggac gcccacgcgg tcgagaccag
     2161 ctgcgagacg gacagtcccg atgcgaggac cttggccttg agggcggcga tgtccgccgc
     2221 gccgatcaac tcgtgcgcga ccggggggac cgggtcctgc cacaactgcg gctccctggg
     2281 aaccagcttg ccgaggtacc gcgaggtggg acccatgtcg cggtgcgtca gcttgaacca
     2341 cgccctggcg aaggcgtcgg cgaactgatc cgggtgctcg tagaagcggc gcgaaatctt
     2401 ctcgtacgcg ggatcgaggc gcagcgagag gtcggtcgtc agcatggttg gcgcgtggcg
     2461 cctggacggg tcgtgggcat ccggaatcgt gccggcgccc gcgccgccct tcgcgatcca
     2521 ctgattcgcg ccggcgggac tcttcgtcag ctcccattca tagccgaaca ggttctcgaa
     2581 gaagttgttg ctccacttcg tcggcgtgga cgtccagatg acctcgatgc cgctggtgat
     2641 cgcgtcggcg cccctgcccg tgcgatagct gttcttccag ccaagcccct gcatctcgag
     2701 gggagccccc tcgggttcag ggccgacgtt ggtcgcaggc gccgcaccgt gcgtcttgcc
     2761 gaaggtatgt ccgccggcga tgagcgccac cgtctcctcg tcgttcatcg ccatgcggcg
     2821 gaacgtctcg cgaatgtccc tcgcggcggc aatcggatcg ggattggcgt tgggcccttc
     2881 cgggttcacg tagatgaggc ccatctgcac ggccgccagg ggattctcga gttgacggtc
     2941 gccgctgtag cgctcgtctc cgagccaggt gcgctcagag ccccagtaga cgtcctgctc
     3001 gggctcccag acgtcctcgc gccctcccgc gaagccgaac gtcttgaagc ccatcgactc
     3061 gagggcgcag ttgcccgtga agaccatcag gtcggcccac gagatcttcc ggccgtactt
     3121 ctgcttgacc ggccagagca gccggcgcgc cttgtcgagg ttcgcgttgt ccggccagct
     3181 gccgagcggc gcgaagcgca gcgtgcccga tcccgctccg ccgcggccgt cgtgaatacg
     3241 gtatgtaccc gcgctatgcc acgccatacg gatgaagagc ggcccgtagt ggccgtagtc
     3301 cgccggccac cagtcctgcg acgtcgtcat caccttctcg atgtccttct tcagggcgtc
     3361 gagatcgacg gtcttgaact cttcggcgta gttgaagtcc ttgcccatcg gatcgcccgc
     3421 gggcgggttc tggtggaggg ccttcaggtg cagctgcttc ggccaccact ctcgattcga
     3481 cgtgccgctg tgggctttgc cagagaaggg acacttgctt tcgttactca tcgacgttga
     3541 cctcttccag gaatgggagt gcccggctca gtccgggcgt tttcttgtgg cccgctgacg
     3601 gggccgtcgg gatgaatgtg cgggggacgc cgtgtccgcc ctcaggcgga ctcgttgatg
     3661 cgcgcgggat gactttcggg acctcacctg gcaatctggg caccgacccc agtacacgac
     3721 ttcggcttcg tcgatctcgt aacccccctc gttcgccgcg gcgagacagg gggccgagcc
     3781 gaccgcgcag tcgacgtcca ccatgcggcc gcagacgcgg cagatcaggt ggtggtggtt
     3841 gtcgcccacc cgggtctcga atcgcgccgg cgacccggag ggctgaatgc gccggacgag
     3901 gccttcggcg accaggacgc caaggcagtc gtacaccgac tggagcgaga tggccccgat
     3961 ctcggccctg acggattcgg ccacggcgtc ggccgcggca tggggctgac ccgcgacggc
     4021 ccgcaagaca gccaatcgct gggccgtcac ctgaatgccg cgctggcgaa gaaggtccgc
     4081 tgggtccgaa gccacgggag tcatattaaa tcaaataatt gagtgattca aatattgtga
     4141 atactcgtgg cgaagcggca tccctcctgc gctagaatcg ccagcgcttc ccctctccta
     4201 ctctttgtga ggactcgacg atgatgcgac ggtttgtgtt ggcagtagtg ctcgtgtcag
     4261 tggcctccgc ggttcacgcc caggagcccg tggttggcgt gaaggatccc gagtcgttgt
     4321 tcaaggacgc ggatccggtg ctcaaccgca acaagcaggc ggcgctgcac atcatgcgcg
     4381 agctgctgca gtgc
//
LOCUS       DBNGSH010000858         5401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3670051, whole genome shotgun sequence.
ACCESSION   DBNGSH010000858 DBNGSH010000000
VERSION     DBNGSH010000858.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3670051"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="AC16CY_19690"
     CDS             complement(<1..679)
                     /locus_tag="AC16CY_19690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3356083.1"
                     /translation="MSSVAELDNVSVRYGRQWALRDITTAFPSGAVGLLGPNGAGKST
                     MIKALLGLLAPDRGAMRVLGLDVRHAPLRIRGRIGYMPESDAHFPQMNAVSFVAHCGE
                     LSGLPAADAMQRAHEVLYYVGLGEARYRNIDQYSTGMKQRIKLAQALVHDPDLLFLDE
                     PTNGMDPKGREEMIELIRDIAHNKGLNLILSSHLLPDVEQTCDHVVVLDKGAVATAGP
                     IAGLKGSG"
     gene            complement(740..1312)
                     /locus_tag="AC16CY_19695"
     CDS             complement(740..1312)
                     /locus_tag="AC16CY_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3356084.1"
                     /translation="MTPLSRDAAWTLLSEWTQGESLRKHALAVEAAVRGYARTFGEDD
                     NDWGVVALLHDFDYERYPTPADHPFRGCEELARRGYPEWVTRAILSHADYSGVPRESL
                     LERTLFACDEMAGFVTAAALVRPSRSVLDLEAPSVVKRMKDKAFARAVSRDDLRNGAQ
                     VLGLPLDEHIGNVIRFMREQADALGLRGSL"
     gene            complement(1309..1770)
                     /locus_tag="AC16CY_19700"
     CDS             complement(1309..1770)
                     /locus_tag="AC16CY_19700"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3356085.1"
                     /translation="MNTTDRRKYPERPYVGVGAVIVRDGQVLLVKRKHEPLKGRWSLP
                     GGAVEIGETLEGCLVREMVEETGLEVGVGPVIEVFDRITRDEAGRVLYHFVLVDYLCW
                     PVGGALRAGSDVADATFADPGELHRYDLTEKAVSVIERAMLVARGNTRAPQ"
     gene            complement(1952..2365)
                     /locus_tag="AC16CY_19705"
     CDS             complement(1952..2365)
                     /locus_tag="AC16CY_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR3356086.1"
                     /translation="MKTATEYLWFNTKKRQEFVRITDEVASVVTKSGVKEGMVLVSAM
                     HITASVYVNDWEGGLIEDFQVWLEKLAPSGLDYRHHQTGEDNADAHLKRTLMGHQVLL
                     PVTAGKLDLGPWEQVFYAEFDGQRRKRVVVKVIGE"
     gene            complement(2383..3447)
                     /gene="tsaD"
                     /locus_tag="AC16CY_19710"
     CDS             complement(2383..3447)
                     /gene="tsaD"
                     /locus_tag="AC16CY_19710"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3356087.1"
                     /translation="MIVLGIETSCDETAAALVEETGDRGRPWRVRSSVVASQNAIHSE
                     WGGVVPELSARQHIRDICGVVERALAEAGVARYDGVAVTQGPGLVGSLLVGLSFAKAL
                     AWSRDVPLIPVHHLAGHIESLVLEQGEMPTPAVVLVVSGGHTSLYLLTSTGALDMLGR
                     TRDDAAGEAYDKVAKLLGLGYPGGPLVDRLAAAGDDLAVPFPKPRLTHADRNAPDVPG
                     RRDFSFSGLKTSVLRYVMGRRAALGLAEASPLPEEEIRDICASFQRVVVGVLLDRLFE
                     AARWYGARSVGIAGGVSANSRLRAEAEARGRARDLPVFIPGLALATDNAAMIAAAGLR
                     RYHAGAIARWDLEADAAMKL"
     gene            complement(3472..4548)
                     /gene="mtnA"
                     /locus_tag="AC16CY_19715"
     CDS             complement(3472..4548)
                     /gene="mtnA"
                     /locus_tag="AC16CY_19715"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR3356088.1"
                     /translation="MLPTIEWKDDVIVMIDQRKLPASEVYVTCRTAKEVAKAITTMVI
                     RGAPAIGVAAAMGIALGMRRSSATGTKKFTTEFQNTCDLLAATRPTAVNLFWAIERMK
                     RTFAEAAHGGSSVAEIAQRLETEARAIHDEDVASCKAMGAHGATLVPDSANILTHCNA
                     GALATAGYGTALGVIRASVAQGKRIAVLADETRPFLQGARLTAWELVKDGIDTTVITD
                     GMSAAMMGLGRVDLVVVGADRIAANGDVANKIGTYGVAVLAKEHGIPFYVAAPISTID
                     LKTPDGSRIPIEERNDREVTHVGPSRLTPEGARIRNPAFDVTPARYVTAIITERGIAR
                     PPYGESLAELAAGGGQQAVRSVRL"
     gene            complement(4646..5041)
                     /locus_tag="AC16CY_19720"
     CDS             complement(4646..5041)
                     /locus_tag="AC16CY_19720"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR3356089.1"
                     /translation="MNASKLIAAARRARRHAQAGYSGFKVGAALEAADGTIITGCNVE
                     NATYGLTVCAERVAMFKALSEGHRKFRRVAVVADTRSPTPPCGACRQVLWEFGGDLEV
                     VLANLRRETGRHRLKDLLPLPFDGRLLNE"
     gene            complement(5028..>5401)
                     /locus_tag="AC16CY_19725"
     CDS             complement(5028..>5401)
                     /locus_tag="AC16CY_19725"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001882 - nucleoside binding [Evidence
                     IEA];
                     GO_function: GO:0002060 - purine nucleobase binding
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0047975 - guanosine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR3356090.1"
                     /translation="GARFPDMTEVYSRRLRGIADEAARARGVPVTHGVYAGLTGPSYE
                     TPAEIRFLRTIGADAVGMSTVPEAIAARHMGLEVLGVSCITNMAAGVLPQPLVHDEVI
                     ETARRVRGSFISLIEGIIERL"
BASE COUNT          865 a         1893 c         1764 g          879 t
ORIGIN      
        1 cgccggaacc cttcaggccc gcgatcggcc cggccgtggc aacggcgccc ttgtccagca
       61 cgacgacgtg gtcgcaggtc tgctcgacgt cgggcagcag atgcgacgac agaatcaggt
      121 tcagcccttt gttgtgggcg atgtctcgaa tcagctcgat catctcctcg cgccccttcg
      181 gatccattcc gttggtgggc tcgtcgagaa agagcagatc cggatcgtgc acgagcgcct
      241 gtgcgagctt gatgcgctgc ttcatgcccg tcgagtactg gtcgatgttc cgatagcgcg
      301 cctcgcccag tccgacgtaa tagagcacct cgtgcgcccg ctgcatggcg tcggcggcgg
      361 gcaggcccga cagctcgccg cagtgcgcca cgaacgagac ggcgttcatc tgagggaaat
      421 gcgcgtcgct ctcgggcatg tacccgatgc gtccgcggat tcggagcggc gcgtgccgga
      481 cgtcgagccc cagcacgcgc atcgccccgc gatcgggtgc gagcaggccc aggagcgcct
      541 tgatcatcgt gcttttgccg gccccgttcg gtccaagcag acccaccgcg cccgagggaa
      601 aggcggtcgt gatgtcccgg agggcccact gtcttccgta ccggacgctg acgttatcga
      661 gctcggcgac tgaggacatg gctcacgcgg ggttcacggt gaccaatacg atggccgagg
      721 ttcaaaaggt tcaagcggct catagacttc cccgcaatcc cagcgcgtcg gcctgctcac
      781 gcatgaatct gatgacgttg ccgatgtgct cgtcgagcgg caggccgagc acctgggccc
      841 cgttccgcag atcgtcgcgg gagacggcac gcgcgaaggc cttgtccttc atccgcttga
      901 cgacgctggg cgcctccagg tcgagcacgc tgcgggaggg ccggacgagc gcggccgccg
      961 tgacgaagcc ggccatctcg tcgcaggcga acagcgtccg ctcgagaagg ctctccctcg
     1021 ggacccccga atagtcggca tgggacagaa tcgcgcgcgt gacccactcg ggatagccgc
     1081 gccgggctag ctcctcacag cctcgaaacg ggtgatcggc gggcgtggga taccgctcgt
     1141 agtcgaagtc gtgcagcagc gccacgacgc cccagtcgtt gtcgtcctca ccgaaggtgc
     1201 gggcatagcc gcgcacggcc gcctccacgg cgagcgcatg cttccgcagg ctctcccctt
     1261 gagtccattc cgacaacagc gtccacgccg cgtcgcgcga gagcggtgtc attgcggcgc
     1321 ccgggtgttg ccgcgcgcca ccagcatggc tcgctcgatg acggacacgg ccttctcggt
     1381 cagatcgtaa cggtgcagct cgccgggatc ggcgaaggtc gcgtccgcca cgtccgagcc
     1441 cgcgcggagc gcgccgccaa ccggccaaca caggtagtca accaggacga agtggtacag
     1501 aacccggcca gcctcgtcgc gagtgatccg atcgaacacc tcgatcacgg ggcctacgcc
     1561 aacctcgaga ccggtttcct cgaccatctc gcgcacgagg cagccctcga gcgtttcgcc
     1621 gatttcgacg gcgccgcccg gcagactcca tcgccccttg agcggctcgt gcttgcgctt
     1681 gacgagcagc acctgtccgt cccgaacgat caccgcgccg acgccgacgt acgggcgttc
     1741 agggtacttg cgtctatcgg tagtgttcac acttctctct tcacacttct cggttcgtgt
     1801 tcaatttcgg agttcgtgtt cgagagttca gcgttcagcg ttcccggttc ggggccggat
     1861 tctccaggga acaacgaacc gcgaaccttc tgaacacgaa cctgaaactg aacacgaacc
     1921 gagaagcgag aaccgggaag cgtgaacggt actattcacc gatcactttg acaacgacac
     1981 gtttccgccg ctgcccgtcg aattccgcgt agaacacctg ctcccagggg cccagatcga
     2041 gcttgcccgc ggtgacaggc agcagcacct gatgtcccat cagcgtgcgc ttcaggtgcg
     2101 cgtccgcgtt gtcctccccg gtctggtgat gccggtagtc gagccccgac ggcgcgagct
     2161 tctcgagcca cacctggaag tcctcgatga gcccgccctc ccagtcgttg acgtagaccg
     2221 aggcggtgat gtgcatggcc gagacgagga ccatcccttc tttcaccccg ctcttcgtca
     2281 cgacactggc aacctcgtcg gtgatgcgga cgaactcctg ccgcttcttc gtgttgaacc
     2341 agaggtattc ggttgctgtc ttcatgggtc tccggtctac ggtcagagct tcatcgccgc
     2401 gtcggcttcg agatcccagc gtgcgatcgc gcccgcgtgg tagcggcgca ggccggcagc
     2461 cgcgatcatc gccgcgttgt cggtcgcaag cgcaagcccc gggatgaaga cgggaagatc
     2521 gcgagcccgg ccgcgcgcct ccgcttcggc ccgcagccgg ctgttggccg acacgccgcc
     2581 ggcgatgccg acgctgcggg ccccgtacca acgcgccgcc tcgaagagcc ggtcgagcag
     2641 cacgccgacg accactcgct gaaagctggc gcagatgtcg cggatctctt cctccggcag
     2701 cgggctggcc tcggcgaggc cgagcgccgc gcgccgcccc attacgtatc gcaggacgga
     2761 cgtcttgagt ccgctgaaac tgaagtcgcg ccggccgggc acatcggggg cgtttcggtc
     2821 ggcgtgcgtc agccgcggtt tcgggaacgg cacggcgaga tcgtcgcccg ccgcagccag
     2881 gcgatcgacg aggggccccc cggggtatcc cagcccgagc agcttggcga ccttgtcgta
     2941 ggcctcgccg gcagcgtcat cgcgagtgcg gccgagcatg tccaacgcgc ccgtcgacgt
     3001 cagcagatag agactggtgt gcccccccga gacgacgagc acgacggcgg gcgtcggcat
     3061 ctcgccctgc tcgaggacga gagactcaat gtggcccgcg agatgatgca ccgggatcag
     3121 cgggacatcg cgcgaccacg cgagcgcctt ggcgaacgac aagccgacga ggagcgaccc
     3181 gaccaggccc ggcccttgcg tcacggccac gccgtcgtaa cgggcaacgc ccgcctcggc
     3241 gagcgcgcgc tccacgacac cgcagatgtc gcggatgtgc tgccgcgccg agagctcggg
     3301 aacgacgcct ccccactcgc tgtgaatcgc gttctgcgac gccacgacgc tcgatcgcac
     3361 gcgccacggg cggccccggt cgccggtttc ttccaccagc gccgccgcag tctcgtcgca
     3421 cgaggtttcg atgccgagaa cgatcatcgt ttcggatccg gcacggtccg gctaaagccg
     3481 gacactacgt accgcctgct gcccgccgcc ggctgccaac tcggccagcg attcgccata
     3541 cggcggcctg gcgatgccgc gctcggtgat gatggccgtg acatacctgg ccggggtcac
     3601 gtcgaacgcc ggattacgga tgcgcgcgcc ttcgggcgtc aaccgggacg gcccgacgtg
     3661 cgtcacctcc cggtcgttgc gctcctcgat tggaatccgg ctgccgtcgg gagtcttcag
     3721 atcgatcgtg gaaatgggcg cagcgacgta gaacgggatg ccgtgctcct tggcgagcac
     3781 cgccacgcca taggtgccaa tcttgttcgc gacgtcgccg ttcgccgcga tgcggtccgc
     3841 accaacgacg acgagatcga cgcggccgag ccccatcatc gcggccgaca tgccgtcggt
     3901 gatgaccgtg gtgtcgatcc cgtccttgac cagctcccag gccgtgagcc tggcgccttg
     3961 caggaagggg cgcgtctcgt cggccaggac cgcgatccgc ttgccctgcg cgaccgacgc
     4021 acgaatgacg ccgagcgcgg tgccgtagcc ggcggtggcc agcgccccgg cgttgcagtg
     4081 cgtcaggatg ttggccgagt cgggcacgag cgtggcgccg tgcgcaccca tcgccttgca
     4141 gctcgcgacg tcctcgtcgt gaatcgcccg cgcctcggtc tcgagccgct gcgcgatttc
     4201 cgcgacggag gacccgccgt gcgcggcctc cgcgaacgtc cgcttcatgc gctcgatcgc
     4261 ccagaagaga ttcacggcgg tcggacgcgt ggccgcgagc agatcgcacg tattctgaaa
     4321 ctcggtcgtg aacttcttcg tcccggtcgc gctgctccgg cgcatgccga gcgcgattcc
     4381 catcgccgcc gccacgccaa tggccggcgc gccgcggatg accatcgtgg tgatcgcctt
     4441 ggccacttcc ttggccgtcc tgcatgtcac gtagacctcg gaagcaggca gcttccgctg
     4501 gtcgatcatg acaatcacat cgtctttcca ctcgatggtg ggcagcatgt ctcgggatga
     4561 tatcaagcag ggctcagggc ccaggcctca gggccaggcc tggggcctcc gacagccctg
     4621 aggcctgagc cctgggccct gaggcctact cgttgagcag ccgcccgtcg aacggcagcg
     4681 gcagcagatc cttcagccga tgccgcccgg tctcgcggcg gaggttggcg agcaccactt
     4741 cgagatcgcc gccgaactcc cagaggacct gccggcacgc gccgcacggc ggcgtcggcg
     4801 agcgggtgtc ggcgacgacc gccacgcgac ggaactttcg gtgcccctcc gagagcgcct
     4861 tgaacatggc gacgcgctcg gcgcacaccg tcaatccgta tgtcgcgttc tcgacgttgc
     4921 agccggtgat gatcgttccg tcggcagctt cgagcgccgc gccgaccttg aatcccgaat
     4981 agccggcctg cgcgtggcgg cgcgcgcggc gggcggcggc aatcagctta gaggcgttca
     5041 atgatgccct cgatcagcga gatgaacgat ccgcgcacgc gccgggccgt ttcgatgact
     5101 tcgtcgtgga cgagcggctg cggcagcacg ccggcggcca tgttcgtgat gcacgagacg
     5161 ccgagcacct cgagccccat gtgccgcgcg gcgatcgcct ccggcaccgt ggacatcccg
     5221 acggcgtcgg cgccgatcgt cctgagaaac ctgatctccg cgggcgtctc gtagctcggg
     5281 ccggtcaggc cggcgtagac gccatgcgtc accgggacgc cgcgcgcgcg cgccgcctcg
     5341 tcggcgatcc cgcgcagccg gcgtgaatac acctcggtca tgtccggaaa gcgcgcgccg
     5401 a
//
LOCUS       DBNGSH010000859         9704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3839149, whole genome shotgun sequence.
ACCESSION   DBNGSH010000859 DBNGSH010000000
VERSION     DBNGSH010000859.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3839149"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC16CY_19730"
     CDS             complement(<1..342)
                     /locus_tag="AC16CY_19730"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3356091.1"
                     /translation="MPAPRLYRVIVPVDDLERARRFYTTLLGEPGFGVSPGRHYINCG
                     GVILSLYSPRADGDPVDPRPNFEHIYFAVADLEAAYRRAESVGGLSKETGDGNLPMGQ
                     IAKRPWGERSFY"
     gene            417..794
                     /locus_tag="AC16CY_19735"
     CDS             417..794
                     /locus_tag="AC16CY_19735"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3356092.1"
                     /translation="MTRTGDSYRERASCPLYTAIGVIDGRWKPMIFQRLTSRPHGFGE
                     LRRAMPRVTAKVLREQLRQMQADNLVVRHALTPAALGVRYQLTPYGRTLGPVFETLWQ
                     WGVRHLARSSAERGTLVSPPAGA"
     gene            910..2034
                     /locus_tag="AC16CY_19740"
     CDS             910..2034
                     /locus_tag="AC16CY_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356093.1"
                     /translation="MRRRHVERCQMRALGTLVVTGTLGLGAATASAQIKPAIEIRPPV
                     VGDCVVILVPKALESGSIVDLELDYGYLPRHRVVDPSRNIEFPVRIPLQKGNRLRALV
                     NGVAVQPDLLVGATPPADRKPEGTCDGGDEDEDEERETSFAAEAYLGGIVDTFAPDAV
                     GNYQNPEAGARKTRMTFGIDFDGRLYGKDNDRVQFWVFAETLYGVRSADIDCSGQDKP
                     PVCSDDPSAKALYILEHATSLEAFINPRLQFLRLQTHSESPVVLYVTGRLGFIALDGA
                     PSVFKNHEVGAGFLLDGGPFQGSYLDFGWGKNELIADARWNRLKIDGTLVFSLERLLK
                     NGATGFFIRLRVDNDLRGESADSIQTSYGMSFDLGGLFAP"
     gene            2043..3863
                     /locus_tag="AC16CY_19745"
     CDS             2043..3863
                     /locus_tag="AC16CY_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356094.1"
                     /translation="MRTQSASHRLPIAAAAAVLVGMIAPAPAQESPKTVQTAFWQIDP
                     EDWHKTGRAEFPERQKLDKERPQDVGVAFSGGGTRSAAATIGQLRGLRQNGWLDRVRY
                     VTAVSGGSWAAVPYTFYDGPLEELLGEYVPTNSLTPEALTRAASGKLAERVARSELGH
                     VGLQEVPQFLPDRVAGYDTTTLQSAVSTLRRALRKWRGQNLPEPDRRDKSYAHMLGQV
                     FLGKHEDLDPLVARGNLAPYTWDRETAIEMSKVTGYFSGDFVHAAANRPFLIVGGTLI
                     WQHPAFTYPRLIPVEYTPLYTGIRQRFGGIGGTYVWPWAYDRSVVARQPGDRMLVSPG
                     PDGRMFTLADVIASSGAAPQLTLLLGTGVPSRLRASLRTAAEAFPSFANTAVRDGEPI
                     PTGDLFPHGDGGFTDNFGLMPLLARQVRNIIVFVNSNKTWTLNDQLQSFFIPVDVKDG
                     GGDKSMNAVFEASRYKELLDGLDAATASGGPAVYCGRQTWSVHGNELYNIRTYDDLKI
                     CWVYNHVAGEWKKRLDATIRGWLDWTKASGTPRTDEVKALESFPYYATFGENRPRVIR
                     LNPLQINLLADLAAWSITNTATVDAITGAFGEGVLPKPTR"
     gene            complement(3921..5009)
                     /gene="tcuB"
                     /locus_tag="AC16CY_19750"
     CDS             complement(3921..5009)
                     /gene="tcuB"
                     /locus_tag="AC16CY_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HIR3356095.1"
                     /translation="MRVDDLLDHGAHVASVCNACRYCEQFCPVFPAIEQRLFFEKRDL
                     VYLANLCHNCGECLYSCQFAPPHEFGIDVPRTMARIRERTYEEYAWPRWLASALRRNG
                     PTSALTLAGVMAALFLAAVWVTNPQVLWQPGITADFYSVVPHAVMVALFGAVGLMVLM
                     ALSIGGIRFWREAAQSGAFTAAALAAALRDALTLRHLHTPDAECVSGEEQRTPWRRRF
                     HHFTFYGFALCFASTTVAAVYHELGFEAPYAYASVPVILGTAGGFGLLIGPAGLWLLR
                     RRRDPALGGGIDSVGADEAFLVLLVATSLTGLLLLAFRHSAAMSVLLLVHLGAVLALF
                     LTLPYGKFAHGLYRTLALVRYRREVTRD"
     gene            complement(4996..6375)
                     /gene="tcuA"
                     /locus_tag="AC16CY_19755"
     CDS             complement(4996..6375)
                     /gene="tcuA"
                     /locus_tag="AC16CY_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR3356096.1"
                     /translation="MCDVVVVGGGNAALGAALAARLAGARVVVLEAAPRVFRGGNSRH
                     TRNFRCAHDGPTDVLAGAYRDEEFWSDLLEVNGGETNERLARLLIRESAGCPAWMRRF
                     GVRLQPALRGTLHLGRTNAFFMGGGKALLNACYAAAERLGVQVIYDAEVVALDIEDGS
                     FRRAAVLAGGARRTIDGRAVVVAAGGFEANLDWLRDAWGEAARNFVVRGTPYNTGTLL
                     RILLDAGAQPVGDPRACHAVAVDARAPRFDGGIVTRLDSVPFGIVVNRHGRRFSDEGE
                     GLWPKRYATWGMLVAQQPDQIAYSIVDAKAIGRFLPSAFPPIAAGSIGELAAKLQIPA
                     GELESTVAAFNDGVQPGTFDPDTLDDCRTVGLTPDKTHWAQRLDTPPFWGYPLRPGIT
                     FTYLGVAVDDSARVLGSNGRRFDNVYAAGEIMAGNILRHGYLAGIGMTIGTVFGRIAG
                     EAAARHARG"
     gene            complement(6556..8256)
                     /locus_tag="AC16CY_19760"
     CDS             complement(6556..8256)
                     /locus_tag="AC16CY_19760"
                     /inference="COORDINATES: protein motif:HMM:NF012320.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356097.1"
                     /translation="MHILLLSAGGGGGNILRSVKASFRRDLAVTQKTDPKYAERLRRA
                     VTTRFLDTNEFALSDLPREERFLIGARTTGRLGARHDPEVALQALNESRGEIEVLLSR
                     YSIIILIGTGGKGTGSGTMFPLAQIARQQRKLVIPIFVRPSFERHEVDKRHYDYAVKA
                     IEQFDAANIRLIEILNDRGYAEHDPQPQPVVWERMNLPIARSLRGLIYVLWDLSQVDP
                     SDLSILFAGAGRLRLGFAEIDPPEGAEPSNEQVEHAARRCCENPYYAFHKPAGTSLIC
                     IQGDWSNLVDAKIKGQLAAAMRVGAKSPYSPLYARAVGTPKPWGVTTLFAEYTGAHEP
                     LEVDWSLERGVRATATSHRTVPEPLDSPADLSLARGKPAAVEDALPVAPLDSRIDETL
                     ARGGAIDSEADHSPTPTRAAEARPFTTFWEFAVALNRSDAAALALAGNGASDLPIDGV
                     ELRKLLGLMWFRTALGRLSQGWRDRILDVLIDSATVPDHAVKFGRQTVRLRDLSYEQL
                     QEIGAKTIVSDVIQPDLDLLITVGRFWGPDAVTRLHFVDAPERQEPSMMENLLQKFRN
                     "
     gene            complement(8238..9089)
                     /locus_tag="AC16CY_19765"
     CDS             complement(8238..9089)
                     /locus_tag="AC16CY_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356098.1"
                     /translation="MLAASSAVFAQSSLVCHPIRLGESAEDVARRITGQARNTYQAWF
                     QIMNASSRFVPKSQYDRVRAGWRACITKPAVESVPAASDQVRSIEVLDAFAAQDAPGA
                     EALATAAALVSAAHVPQPEASGILRAIRSVDLTIVWLGAAVAVSWAGWRVFDGYSARR
                     NTASIVVKHFTTRFVHEFERPLVRSHAGDRAVRSGFRRKRRGRFDILLAPGEGRRYPN
                     LSDHRKNVEYDVVRVLGLLADESFESGPLYTQAGWVVVPFQTRNGRRVRLQPDHGPAG
                     VTCISSF"
     gene            complement(9304..>9704)
                     /locus_tag="AC16CY_19770"
     CDS             complement(9304..>9704)
                     /locus_tag="AC16CY_19770"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3356099.1"
                     /translation="IGNLALLVLNLPLAGVWARVMLVPRPLLFAGILVLASVGAYSLN
                     RSLLDLALMYAVGAVGCAMRIYDVPLVPAVLGLVLGPLSEQQFRRAVAISEGDLTVFM
                     SRPISAALLALAIVVLVGPRLVDRLARRRR"
BASE COUNT         1695 a         3231 c         3221 g         1557 t
ORIGIN      
        1 gtagaaggat cgctcccccc acggccgctt cgcgatctgg cccatcggca ggttgccgtc
       61 gccggtctcc ttcgacagcc cgccaacgga ttcggcgcgt cgatacgcgg cctcgaggtc
      121 ggcgaccgcg aagtagatgt gctcgaagtt cggccgtgga tcgaccgggt cgccgtcggc
      181 cctcgggctg tagagcgaaa ggatcacgcc gccgcagttg atgtagtgcc gtcccggcga
      241 gacgccgaag ccgggttccc ccaggagcgt cgtgtagaag cgcctggcgc gctcgaggtc
      301 gtccaccgga acgatgacgc gatagagacg tggcgcaggc atgggacgaa ctgtagcgat
      361 cgatccggtc gccggcaaga cggtactttg atgtaccctc gtcgcggagg cggaccgtga
      421 cgcgcaccgg agacagctat cgagagcggg cgtcgtgccc gctctacacc gcaatcggcg
      481 tcatcgatgg acgctggaag ccgatgatct tccagcggct gacgtctcgc ccgcacggtt
      541 ttggagagct gcggcgcgcc atgccgcgcg tcacggccaa ggtcctgcgc gagcagcttc
      601 gacagatgca ggccgacaac ctcgtcgtcc ggcacgcgct cacgcccgcg gcgctcggcg
      661 tgcgctatca gctcacgccg tacggccgca cgctcgggcc ggtattcgaa acgctctggc
      721 aatggggcgt tcgacacctc gcacggagca gcgccgaacg ggggacgctc gtctcgcccc
      781 cggccggggc ctgaccgtgg gacgacccga tcgactgacg gtcccgagct gttcggagtc
      841 tttcccttgc acgtctatca cctaccgaga ccacaacccg gattcgcgtc catcgagagg
      901 ggagagctta tgcggcgccg gcacgtcgag cgatgtcaga tgagggcgct gggtacgctg
      961 gtggtcaccg gcacacttgg actcggcgcg gctactgcat cagcccagat caagccggcg
     1021 atcgagattc gaccgcccgt cgtcggtgac tgtgtcgtta tcctggtccc caaggcgctc
     1081 gaatccggtt ccatcgtgga cctcgaattg gactacggct accttccacg gcaccgggtc
     1141 gtggatccgt cgcgcaacat cgagttcccc gtgcgcatac ccctgcagaa gggcaatcgc
     1201 cttcgagcgc tcgtcaacgg cgtggcggtc cagcccgatc tccttgtcgg ggcaactccg
     1261 ccggctgacc gaaagccgga aggcacgtgc gatggcggag acgaagacga agacgaagag
     1321 cgagagacgt ccttcgctgc cgaagcctat ctgggcggca ttgtcgacac gttcgccccg
     1381 gacgccgtcg gcaattatca gaaccccgag gccggggcac gaaagacgcg gatgaccttc
     1441 ggaatcgact tcgatggccg cctctatggg aaagacaacg accgcgtgca gttctgggtt
     1501 tttgccgaga ccctgtacgg cgtgcggtcc gccgacatcg actgttccgg ccaggacaag
     1561 ccgccggtct gcagtgacga cccgtccgcc aaggccttgt acatcctcga acatgccacg
     1621 agcctggagg cgttcatcaa tcccagactc cagttcctga ggctgcagac gcactccgaa
     1681 tcgcctgtcg tgctctatgt caccggccgc cttggcttca tcgccctgga tggggcgccg
     1741 agcgtcttca agaaccacga ggtcggagcg gggttcctcc tcgatggcgg cccattccag
     1801 gggagctatc tggatttcgg atggggcaag aacgagctga ttgcggacgc gcgctggaat
     1861 cgactgaaga tcgatggcac tctcgtgttc agcctcgagc ggctgctgaa gaacggcgcg
     1921 accggtttct tcattcgcct gcgggttgac aacgaccttc gcggggagtc ggcagacagt
     1981 atccagacgt cgtatgggat gtcgttcgat ctgggcggcc ttttcgcacc atgaggcggg
     2041 tcgtgcgcac gcagtcagca agccatcgtc ttcccatcgc cgccgcggcc gcggtgctcg
     2101 tgggcatgat cgctccggcg ccggcccagg aatccccgaa gacggtccag acggcgttct
     2161 ggcagatcga ccccgaagac tggcacaaga ccggtcgtgc agagtttccg gagcgccaga
     2221 agctcgacaa agagcgccct caagatgtcg gtgtcgcgtt ttccggcggc gggactcgct
     2281 cggcggctgc cacgatcgga cagctccgag ggctgcgcca gaacggctgg ctcgaccgcg
     2341 tgcgctacgt cacggccgtg tccggcggct cctgggcggc cgtgccatac accttctacg
     2401 acggcccgct ggaggagctg ctcggagagt acgtgccgac gaattcgctg acgccggagg
     2461 ccttgacccg tgcggcctcg gggaagctcg ccgagcgtgt cgcgcgttcc gagctcggtc
     2521 acgtcggcct ccaggaggtt ccccagttcc tcccggatcg agttgccgga tacgacacca
     2581 cgacgctgca gagcgcggtg tcgacgctgc gacgcgcgct gcgcaagtgg cgtggacaga
     2641 acctgcccga accggatcgt cgcgacaagt cgtatgcgca catgctcggg caggtgttcc
     2701 tcggcaaaca cgaggatctg gatcctctcg tcgcacgcgg caatctggcg ccctacacgt
     2761 gggatcgcga gacggccatc gagatgagca aggtcacggg ctacttctcg ggagacttcg
     2821 ttcacgccgc cgcgaaccgt ccctttctga tcgtcggcgg cacgctgatc tggcagcatc
     2881 ccgcctttac gtacccccgg ttgattccgg tcgaatacac gccgctctac acgggcatcc
     2941 gccagcggtt cggcggcatt ggcggaacct acgtctggcc gtgggcctac gatcggtcgg
     3001 tcgtggcccg ccagccgggc gacagaatgc tggtgagccc cggtccggat gggcggatgt
     3061 tcacgctcgc agacgtcatc gccagcagcg gggcggcgcc gcaactcacg ttgttgctgg
     3121 gtacaggggt acccagccgg ctgcgcgcgt ccctccggac ggcagccgaa gcctttccct
     3181 ctttcgccaa cacggccgtc agggacggcg agccgattcc tacgggcgac ctctttccgc
     3241 acggcgacgg aggattcacc gacaacttcg gtctgatgcc gctgctcgcg cgacaggtgc
     3301 gcaacatcat cgtcttcgtc aactccaaca agacgtggac gctgaacgat cagctccagt
     3361 cgttcttcat ccccgtcgac gtcaaggacg gcggaggcga caagagcatg aacgcggtgt
     3421 tcgaggcctc acggtacaag gaattgctgg atggacttga tgccgccacg gcgtcaggag
     3481 gtcctgccgt gtattgcggc agacagacct ggtccgttca tggaaacgag ctgtacaaca
     3541 tcaggacgta tgacgacttg aagatctgct gggtctacaa ccacgtagcc ggcgagtgga
     3601 agaagcggct ggacgcaacg attcggggct ggctggactg gacgaaggcg agcggcacgc
     3661 cgcgaaccga tgaggtcaag gcgctggaga gctttccgta ctacgccacg ttcggagaga
     3721 acagaccgcg cgtcatccgc ctcaatccac tgcagatcaa cctgctcgcg gatctggccg
     3781 cctggtcgat taccaacacc gcgacagtcg atgccattac cggcgccttt ggcgaaggcg
     3841 tgctcccgaa gccgacacga tgagtcgagg ccacatgagt tgcgcagaga tctgatgctc
     3901 gagttcggtg gcctccgggg ttaatcacgt gtgacctcgc gtcggtacct caccaaggcc
     3961 aacgtccgat acaggccgtg ggcgaacttg ccgtacggca gcgtcaggaa cagcgcgagc
     4021 acggcgccga ggtgcacgag cagcagcacg ctcatcgcgg cgctgtgacg gaacgcgagc
     4081 agcagcaagc ccgtaaggct cgtcgcgacg agcaggacga ggaacgcctc gtccgcgccc
     4141 actgagtcga tgccgccgcc aagtgcagga tcgcgccggc ggcgaagcag ccacagtccc
     4201 gcggggccga tcagcaatcc gaatccgcca gcggtcccga gaatcaccgg gacgctcgcg
     4261 tacgcgtacg gcgcctcgaa gccaagctcg tgatacacgg ccgccaccgt cgtcgatgcg
     4321 aagcagagcg cgaagccgta aaacgtgaag tgatggaacc ggcgccgcca tggcgtacgc
     4381 tgctcctcgc cgctgacgca ttccgcatcc ggcgtgtgca ggtgccggag cgtcagcgca
     4441 tctcgcagcg cggcagcgag agcagccgcc gtgaatgctc cggactgggc cgcctcacgc
     4501 cagaatcgga tgcctccaat cgagagggcc atcagcacca tcagcccaac ggcgccaaac
     4561 aaggcgacca tcaccgcgtg cgggacgacg ctgtagaagt ctgcggtgat ccccggctgc
     4621 cacagtactt gcgggttcgt cacccacacc gccgcgagaa agagggcagc catcacgccc
     4681 gcgagcgtca atgcgctcgt cgggccgttg cggcgaagag cggacgcgag ccagcgcggc
     4741 caggcgtact cctcgtaggt gcgctcccgg atgcgcgcca tcgtccgcgg cacgtcgatc
     4801 ccgaactcgt gcggcggcgc gaactgacac gagtagaggc actcgccgca gttgtgacac
     4861 aggttcgcca ggtagacgag gtccctcttc tcgaagaaga gacgctgctc gatcgccgga
     4921 aacacgggac agaactgctc gcagtagcgg cacgcgttgc acacgctcgc gacgtgcgcg
     4981 ccgtggtcga gcagatcatc cacgcgcatg ccgcgccgcc gcctcccccg caatccggcc
     5041 gaacaccgtg ccgatcgtca tcccgatccc ggcgaggtac ccgtgccgca ggatattgcc
     5101 tgccatgatc tcgccggcgg catagacgtt gtcgaaccgc cggccgttcg atccgagcac
     5161 gcgcgccgag tcgtccacgg cgacgccgag atacgtgaac gtgattccgg gacggagcgg
     5221 atatccccag aaggggggag tgtcgaggcg ttgcgcccag tgcgtcttgt cgggcgtgag
     5281 cccgacggtc cggcaatcat cgagcgtgtc cgggtcgaac gtgcccggct gcacgccatc
     5341 gttgaaggcc gcgacggtcg attcgagctc gcccgcggga atctgaagct tcgccgcgag
     5401 ctcgccgatc gagccggcgg cgatcggcgg aaacgccgac ggcaggaagc gcccgatcgc
     5461 cttcgcgtcc acgatggaat acgcgatctg atcgggctgc tgcgccacga gcattcccca
     5521 cgtcgcgtac cgcttcggcc agaggccttc gccctcgtcg gaaaagcgcc gcccgtggcg
     5581 gttcacgacg atgccgaacg gcacggaatc gagccgcgtc acgatcccgc cgtcgaacct
     5641 gggcgcccgg gcgtccacgg ccacggcgtg gcatgcgcgc ggatcgccca cgggctgcgc
     5701 gcccgcgtcg agcaggattc tgaggagcgt gcccgtgttg tacggtgtgc cgcgtaccac
     5761 gaagttgcgc gcggcctcgc cccaggcgtc acgcagccag tcgagattgg cttcgaatcc
     5821 gccggcggcg acgacgaccg cacgtccgtc gatcgtcctg cgtgcgccac cggccagcac
     5881 ggctgcgcgc cggaacgagc cgtcctcgat gtcgagcgcg acgacttcgg cgtcgtagat
     5941 cacctgcacg cccaggcgct cggccgccgc gtagcaggcg ttgaggagcg ccttgccgcc
     6001 gcccatgaag aacgcgttgg ttcgccccag gtgcagcgtg ccgcgcagcg cgggctgcaa
     6061 gcgcacgccg aaccggcgca tccacgcggg acatccggca gattctcgaa tcaggagccg
     6121 cgcgagtcgc tcgttggtct ccccgccgtt gacctccagc aggtcggacc agaactcctc
     6181 gtcgcgatac gcgccggcga gcacgtcggt cgggccgtcg tgcgcgcacc ggaaattacg
     6241 ggtgtgccgg ctgttgccgc ccctgaagac gcgcggcgcg gcctccaaca cgaccacgcg
     6301 ggcgccggca agacgcgcgg cgagcgcggc acccagcgcg gcgttgcctc cgccgaccac
     6361 gaccacgtcg cacacgtcct tagcccgtta tgcggcccag agcgcgaatt gtgtactcgt
     6421 tgttgatccg catgacgggc tagccgcgcg gagcgcggca agaagacact gccgactaat
     6481 caagggcgcg aacgggatat caagggcgcg cccgtgatta gctcgattgt cgcacgggcc
     6541 ccgcccgcca caaggtcagt tccgaaactt ctgcagcagg ttctccatca tcgacggttc
     6601 ctgccgctcc ggtgcgtcga cgaaatgaag gcgggtcacg gcgtcaggtc cccagaaccg
     6661 gcccacggtg atcaacagat ccaggtccgg ttgaatcaca tccgacacga tcgtctttgc
     6721 gccgatctcc tgcagctgct cgtagctgag atcgcggaga cgcaccgtct gccggccaaa
     6781 cttgaccgcg tggtcgggca ccgtcgcgct gtcgatcagg acgtcgagga tccgatcgcg
     6841 ccagccttgg gagagtcggc cgagcgccgt gcggaaccac atcaggccga ggagctttct
     6901 cagctcgacg ccgtcgatgg ggaggtccga ggcgccgtta ccggcgagcg caagcgccgc
     6961 cgcatctgaa cggttgaggg cgacggcgaa ttcccagaac gtcgtgaacg ggcgcgcctc
     7021 tgccgccctg gtcggagtgg gcgagtggtc ggcctccgag tcgatggccc cgccccgagc
     7081 gagcgtctcg tcgatgcgcg agtcgagggg cgccaccggc aatgcgtctt cgacggcggc
     7141 cggtttaccc cgagcgagcg acaggtcagc gggcgagtcg aggggttcgg gcacggtgcg
     7201 gtgcgaggtg gccgtcgcgc gaacgcctct ctccaggctc cagtccactt cgagcggctc
     7261 gtgcgcgccc gtgtattcgg caaacagcgt cgtcacaccc cacggcttcg gcgttcccac
     7321 ggccctggcg tacagaggac tgtaggggct cttggcgccg acgcgcatcg cggcggcaag
     7381 ctgtcccttg atcttcgcgt ccacgaggtt cgaccagtcg ccttgaatgc agatcaggga
     7441 tgtccccgcg ggcttgtgga acgcgtagta cggattctca cagcaccggc gagccgcgtg
     7501 ttccacctgc tcgttgctcg gctcggcgcc ctccggagga tcgatctcgg caaagccgag
     7561 acgcaggcgg ccggctccgg cgaacaggat cgagaggtcg gatggatcca cctgcgagag
     7621 atcccagagc acgtagatca ggccgcggag actgcgcgcg atcggaagat tcatccgttc
     7681 ccagacgacc ggctgcggct ggggatcgtg ctccgcgtat ccgcggtcgt tcaggatctc
     7741 gatcagccgg atgttcgccg cgtcgaactg ctcgatcgcc ttgaccgcgt agtcgtagtg
     7801 ccgcttgtcc acttcgtgcc gttcgaacga cggccggacg aagatgggga tgacgagctt
     7861 cctctgctgg cgtgcgatct gcgcgagagg aaacatcgtg ccggagccgg tgccttttcc
     7921 gcccgtgccg atgaggatga tgatcgagta ccggctgagc agcacttcga tttcgccgcg
     7981 cgactcgttc agcgcctgca gggccacctc cgggtcgtgc ctcgcgccga gccggccggt
     8041 tgtgcgggcg ccgatgagga accgctcctc cctgggcaga tcggacagcg cgaactcgtt
     8101 ggtatccagg aaccgggtgg tcacggcgcg cctgaggcgt tcggcgtact tcgggtccgt
     8161 cttctgcgtc acggcaagat cgcggcggaa ggacgccttg accgatcgca ggatgtttcc
     8221 gccgccgccg cccgcactca aaaggaggat atgcatgtga cacctgctgg cccatggtcc
     8281 ggttgcagcc ggacacgacg accgttcctg gtttgaaacg gcaccaccac ccatcccgcc
     8341 tgcgtgtaga gcgggccgct ctcgaaggac tcgtcggcga ggagccccag cactcggacc
     8401 acgtcgtatt cgacgttctt cctgtggtct gacaggttcg gatagcgcct gccctcgccc
     8461 ggcgcgagca gaatgtcgaa tcgccctcgc cgcttccgtc ggaaccccga tcgcacggcg
     8521 cgatcccccg catgggaccg gaccagcggc cgttcgaact catggacgaa tcgggtggta
     8581 aagtgcttca ccacgatcga cgccgtattc ctgcgagcgg agtagccgtc gaacacccgc
     8641 catccggccc atgacaccgc cacggccgcg ccaagccaga cgattgtgag atcgacgctg
     8701 cggatcgcac gaaggatgcc ggaggcttcg ggttgcggaa catgggcggc gctgacgagc
     8761 gccgcggcgg tggcgagtgc ttcggctcct ggcgcgtcct gcgccgcgaa cgcgtctagt
     8821 acttcgatcg acctgacctg atcggacgcc gcaggcacgc tctcgacggc cggcttggtg
     8881 atgcaggccc gccacccggc gcgaacgcgg tcgtattgcg acttgggcac gaacctcgac
     8941 gaggcgttca tgatctggaa ccacgcctgg tacgtgttcc gggcctggcc ggtgatgcgc
     9001 cgcgcgacgt cttctgcgga ctcgccgagt cgaatcggat ggcagacgag gctcgactgc
     9061 gcgaagacgg cgctcgacgc cgccagcatc agcccaattg tcagaacatc cccggcgatg
     9121 cgtgagatcg gccgcaacgc acacctcatc cgttccggcc tcgtaacgag ggtgccgggc
     9181 ctaactcaag ctgtgtgcca gacttttcgg ttcgcggggt tggtcgtctg gcggattgtc
     9241 caacagtcga gcgtggctat tcagagtcac ggtgtcatct cgtgaggtcg ccgcgatcgc
     9301 gtatcaccgc cggcggcggg ccagccgatc gacgaggcgc ggcccgacga gcaccacgat
     9361 cgccaacgcc aacagcgccg cggagatcgg acggctcatg aacaccgtga ggtcgccctc
     9421 gctgatggcg accgccctgc ggaactgctg ctccgacaat ggtccgagaa ccaggccgag
     9481 taccgccggg acgagcggaa cgtcgtagat ccgcatcgcg cagccgacgg cgccgacggc
     9541 gtacatgagc gcgaggtcga gcagcgaacg attcaggctg tacgcgccga cggaggcgag
     9601 cacgaggatg cccgcaaaga gcagcgggcg cggcaccagc atcacgcgcg cccagacgcc
     9661 ggcgagcggc aggttgagga cgagcagcgc gaggttgccg atgt
//
LOCUS       DBNGSH010000860         2929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_274429, whole genome shotgun sequence.
ACCESSION   DBNGSH010000860 DBNGSH010000000
VERSION     DBNGSH010000860.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_274429"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="AC16CY_19775"
     CDS             complement(<1..680)
                     /locus_tag="AC16CY_19775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3356100.1"
                     /translation="MGEIPIRHVAILGAGGWGTALAVHLARVGHHARLWARDAALVAE
                     MRARGANAVYLPDVRFPANLTVTGDLGEALHAADLVVAAVPSHGTRGILRRAAPHVGR
                     GSIVVSATKGLEQGTLFRMSEVIAQELGGAVSVAVLSGPSFAAELARELPTAVSIGSQ
                     TAAVVERVQHEFKAPYFRLYGTDDVVGVEIGGALKNIIAIAAGVVEGLGLGHNAQAGL
                     ITRGLAEMS"
     gene            complement(677..1279)
                     /gene="plsY"
                     /locus_tag="AC16CY_19780"
     CDS             complement(677..1279)
                     /gene="plsY"
                     /locus_tag="AC16CY_19780"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR3356101.1"
                     /translation="MNAIVAVAFGYLAGSVPFSFLLSRRRGVDLRRAGSGNIGASNVL
                     RTTGVGAAVAAMLLDAAKGTGAVVVAQMLTADLAVTVAAACAAVVGHVYPVWLRFHGG
                     KGVATAAGAFAVLAPPALAVATLVFVVLVSVTRFISAGSIAGAVTLAVVAAVNAPRVV
                     AIGAAVAAFVVLYRHRDNMMRLVAGTERRIGQLVADETSR"
     gene            complement(1276..1851)
                     /gene="thpR"
                     /locus_tag="AC16CY_19785"
     CDS             complement(1276..1851)
                     /gene="thpR"
                     /locus_tag="AC16CY_19785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR3356102.1"
                     /translation="MRLFIALDVGAAIQRAAAAVSEELKLRAARLAPRARVSWVKPER
                     MHVTVRFIGEVDERQFAAIRSAIEPALSAGRFDLTIRGIGSFPPDRPPRVLWAGVAEG
                     LDALRVVEREVGARLDALRVSRTASDYNPHLTLARVKHSAGLRAETLLGGLENVVLGT
                     AAIEAVTLFESRLSSTEPIYLAHGRTLLRAS"
     gene            complement(1848..>2929)
                     /locus_tag="AC16CY_19790"
     CDS             complement(1848..>2929)
                     /locus_tag="AC16CY_19790"
                     /inference="COORDINATES: protein motif:HMM:NF014517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HIR3356103.1"
                     /translation="GPTDDDVTREAVASVLGLPLAEDPEITARLRARFVARGYPGPMP
                     EINRRQAMVPAGARVIENPRGSAPGLWIDHEDRVVLLLPGPPRELRPMLTDLVSGPLR
                     ERSSGRSLVRRVVRVAGLIESHVDEALAPLYREWERQPIPVAATILAAFGSIELHLSA
                     RAEQATSSTFAPRASADKAGEALDAAVAQIVSTLGDVVYSTDGRLLEKVVGDLLAGRG
                     LHIGIAESCTGGLITSRLTDVPGSSRYVDRAVVVYANEAKTEWLGVPAALLGEQGAVS
                     ESVALAMAEGIRINARADVGVGVTGIAGPSGGTPEKPVGTVVIAVSMEGDRRVRTFRF
                     HGEREHVKLQASNAALDMVRRLVDR"
BASE COUNT          478 a         1081 c          953 g          417 t
ORIGIN      
        1 gacatctccg cgagcccgcg cgtaatcagg cccgcctgtg cgttgtgccc gagtcccagt
       61 ccttccacca cgccggccgc gatcgcgatg atgttcttga gcgcgccccc gatctcgacg
      121 ccaaccacgt cgtcggtccc gtacagacgg aagtacggag ccttgaactc gtgctggacc
      181 ctctcgacga ccgcggccgt ctgcgacccg atcgagacgg ctgtcggcag ttcgcgcgcc
      241 agctcggccg cgaagctcgg gcccgagagc accgccacgc tcaccgcgcc gccgagctcc
      301 tgcgcgatga cctctgacat ccgaaagagc gtaccttgct ccagcccctt cgtcgcgctg
      361 acgacaatcg atccacggcc gacgtgcggg gccgcgcgtc gaaggatccc gcgcgtgccg
      421 tgcgacggta cggccgcgac cacgaggtcc gcggcgtgaa gcgcctcgcc gagatcgccc
      481 gtgacggtaa ggttcgccgg gaatcggacg tcaggcagat acacggcgtt ggcgccgcgc
      541 gcgcgcattt cggcgacgag cgccgcgtcc cgtgcccaga ggcgcgcgtg atggccgacg
      601 cgtgccaggt ggacggcgag cgccgtgccc cagccccctg ccccgagaat cgcgacgtgt
      661 cgaatgggaa tctctcctat cgagacgtct cgtcggccac gagctggcca attcgacgct
      721 cggtgccggc cacgaggcgc atcatgttgt cgcgatgccg atagaggacg acgaaggcag
      781 caaccgccgc gccgatggcc acgacgcgcg gagcgttgac ggcagccacc accgcaagcg
      841 tgaccgcgcc agcgatagat ccggcagaaa tgaagcgggt gacgctcacg agcacaacga
      901 agacgagcgt ggcgaccgcg agcgccggcg gcgccagcac ggcgaacgcg ccggcggccg
      961 tggccacacc cttgccgccg tgaaatcgca gccacaccgg gtagacgtgc ccgaccaccg
     1021 cggcgcacgc ggctgccacc gtgacagcga gatcggctgt gagcatctgt gccacgacga
     1081 ctgcccccgt tcccttcgcg gcatcgagca gcatcgccgc cacggccgcg ccaacgcccg
     1141 tcgtcctcag cacgttggac gcgccgatgt tgccgctgcc cgcgcggcgc aggtccacgc
     1201 cgcgccgccg ggaaagcagg aaggagaacg gaaccgatcc ggccagatac ccgaatgcga
     1261 ccgcgacgat ggcgttcatg atgcgcggag gagcgtccga ccgtgcgcga ggtagatcgg
     1321 ctcggtcgag gacagccggc tctcaaacag tgtaaccgcc tcgatagcgg ccgtgccgag
     1381 caccacgttt tcaaggccgc ccaacagcgt ttccgcgcgc aggcctgcgg aatgcttgac
     1441 gcgcgccagc gtcagatgcg gattgtagtc cgacgcggtg cgggagaccc ggagcgcgtc
     1501 gagcctggcg cccacctctc gctcgaccac tcgaagtgcg tcgagtccct ccgcgacgcc
     1561 cgcccacagc acccgtggcg gccggtccgg cggaaagcta ccgattcccc ggatcgtgag
     1621 gtcgaacctg cctgccgaga gcgcgggctc gatcgcggaa cggatcgccg cgaactgccg
     1681 ctcgtcaact tcgccgatga acctgacggt gacgtgcatg cgctccggct tcacccagct
     1741 cactcgcgcc ctcggcgcca ggcgcgcggc tcgcagcttc agttcctcgg acacggcggc
     1801 ggccgcccgc tggatggccg ctcccacgtc gagtgcgatg aacagcctca ccgatcaact
     1861 aacctcctca ccatgtcgag cgcggcattc gaggcttgca gcttgacgtg ttcgcgctca
     1921 ccgtggaatc gaaacgtcct gacgcgccga tccccctcca tcgacacggc aattacgacg
     1981 gtcccgacgg gcttttctgg cgttccgccg ctgggacccg cgatgccggt cactccaacg
     2041 ccgacgtcgg cgcgagcgtt gatcctgatc ccttccgcca tggccagcgc cacgctctca
     2101 ctcacggccc cctgttcgcc cagcagcgcc gctggcactc cgagccactc cgtctttgcc
     2161 tcgttcgcgt acacgacaac ggcccgatcg acgtagcgcg agctaccggg tacgtcggtc
     2221 aaccgggacg tgatcagccc ccccgtgcac gattccgcga tgccgatgtg gagaccgcgc
     2281 ccggccagga gatcaccgac caccttctcg agcaggcgcc cgtcggtgct gtacacgacg
     2341 tcgcccaacg tcgagacgat ctgtgcgacg gccgcgtcca gcgcctcgcc cgccttgtcc
     2401 gccgaagccc ggggggcgaa ggtggacgac gtggcctgct cggccctggc cgacaggtgc
     2461 agctcgatcg agccgaacgc cgccaggatc gtcgccgcaa ccgggatcgg ctgacgctcc
     2521 cactcgcgat acaacggcgc gagcgcttcg tccacgtgtg actcgatcag gcccgcgacc
     2581 cgcacgactc ttctgacgag ggacctcccg gagctcctct cgcggagcgg tcccgacacc
     2641 aggtctgtca gcatcgggcg aagctcgcgc ggggggccgg gcagcagaag cacgactcga
     2701 tcttcgtgat cgatccacag gcctggtgcg ctgcctcgcg ggttctcgat cacccgggcg
     2761 ccggcgggga ccatcgcctg gcggcggttg atttccggca tggggcccgg atagccgcgc
     2821 gccacgaagc gcgcgcgcag gcgggccgtg atctccgggt cctctgccag cggtaagcca
     2881 aggacggacg caacggcttc gcgcgtcacg tcgtcgtcgg tcggtccga
//
LOCUS       DBNGSH010000861         3331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_105294, whole genome shotgun sequence.
ACCESSION   DBNGSH010000861 DBNGSH010000000
VERSION     DBNGSH010000861.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_105294"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC16CY_19795"
     CDS             <1..633
                     /locus_tag="AC16CY_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3356104.1"
                     /translation="VPQDAQAPTLDQMRKAVADLRGGRIPRPASYDTMTAEQKAFVKS
                     VLSGPRGDISGSLGVMMVSPVFGDLAQKTIAYSRFAGREGYSVVPPKLSELAIIMGAR
                     AWNAEYAWYVHRRAAERVGLSAEVIAAIRDGRRPAAMDRDVAAVYDFCNELLTTKRVR
                     DATLQAARAVLGGDRGVIDLVGTLGAYQLVAMMMVVDEFPLPDGVAPELR"
     gene            859..2451
                     /locus_tag="AC16CY_19800"
     CDS             859..2451
                     /locus_tag="AC16CY_19800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR3356105.1"
                     /translation="MTSSRRDFIKTTAATAAALAAGPGLTLEAAAQTVPAPGPDPFVL
                     ELAAEALDAARSAGASYADVRVGRYRRQAVGTRERQITGVSDSESYGLGVRTLVNGCW
                     GFAATSVMSRAGVRQTALEAVTLSKAARAVQKRRVELAPITPVTGTWMTPVRRDPLTV
                     PVEEIVALLLSTNEAALKVKNVRFVSSGLALLREVKTLVTSEGTNVTQTMIRVGPSFS
                     CTATSGADFQSYEAELAPRGLGWEYVASLDMPGNAERWASIAAEKLAAKSVEAGQYDL
                     ILEPTNLWLTIHESIGHATELDRAIGDEANYAGTSFVAPPEKMLGQLRYGPPIMNVQA
                     DRTQEGSLSRVAWDDEGVPADQWLLVERGVFKDYQTTREQVARIQKLTGVTRSHGCSF
                     AESWNAVQFQRMPNVSLLPGDRDIGVDDIVADTSRGVLIRNRGSWSIDHQRYNFQFSG
                     QAFYEVRNGKIAGMLKDVAYQSSTPVFWNSMDLIGGKSSYWMGGAFNDGKGEPSQSNS
                     VSHGCVPARFRKVTILNTARSA"
     gene            2451..>3331
                     /locus_tag="AC16CY_19805"
     CDS             2451..>3331
                     /locus_tag="AC16CY_19805"
                     /inference="COORDINATES: protein motif:HMM:NF040244.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PmbA/TldA family metallopeptidase"
                     /protein_id="HIR3356106.1"
                     /translation="MRNASTQAPGTQRTAAPSIGVPLLSREDARALTARVLALSTADQ
                     TRVTIQSGWSGNTRFADGGITTSGGTTDTTLTVWVTFGKRRASSSTNVLDEASLKRTV
                     DLAARLARLAPEDPELMPELGPQNYTPVDAFVAGTADLDAEARAAAVERAVSAATAAG
                     KPAGAIFTAGYLEANAQAVAVATSNGLFAYHRTTDADFSVTARTPDGTGSGWAHGGSR
                     DWSAVDPAAIGRLAAQKAVASRNPQAIAPGLYTAVLEPQAVTDLIPLLAGALNARSAD
                     EGRSAFSKPGGGTRIGER"
BASE COUNT          549 a         1086 c         1181 g          515 t
ORIGIN      
        1 gtgccccagg acgcgcaggc gccgacgctc gaccagatgc gcaaggcggt cgccgacctg
       61 cgcggcgggc ggattccgcg tcccgcgtcc tacgacacga tgacggccga gcagaaggcc
      121 ttcgtcaaga gcgtcctgag cgggccgcgc ggcgacatca gcgggtcgct cggcgtgatg
      181 atggtcagcc ccgtgttcgg cgacctggcg cagaaaacga ttgcctactc gcgattcgcc
      241 ggacgcgagg gatactccgt cgtaccgccg aagttgtcgg agctggcgat catcatgggc
      301 gcccgcgcct ggaacgcgga atacgcctgg tacgtccacc ggcgcgccgc cgagcgggtg
      361 gggttgagcg ccgaggtgat cgccgcgatc cgcgacggca ggcgccctgc ggcaatggac
      421 cgggacgtgg ctgcggtcta cgatttctgc aacgagttgc tgaccaccaa acgcgtgagg
      481 gacgccacac ttcaagcggc ccgcgccgtg cttggcggcg accgcggtgt gatcgatctc
      541 gtaggcacgc tcggcgccta tcagctcgtg gcgatgatga tggtggttga cgaattccca
      601 ttgccggatg gagtggcgcc tgaactcaga tagaacggga cggagaatgt ggatcgtcgt
      661 tcacacttct cacttctcgc ttctccgttc gtgttcaggt ttcgttcgtg gttcgtggtt
      721 cgtggttcgt ggttcagtgt tcggtgttcg gtgtttaccc cgtggttcga gggacgctcg
      781 cctcgagttg cccgccgaac cgtgaaactg aacacgaact gagaagtaag aagcgagaag
      841 tgtgaacgag ggttcagtat gacttcctct cgccgcgatt tcatcaagac gacggcggcg
      901 acggcggcgg cgctcgcggc ggggcccggc ctgacgctgg aggcggccgc gcagacagtg
      961 cctgcgccgg ggccggatcc gttcgttctc gagttggccg ccgaggcgct cgacgccgct
     1021 cgttctgccg gggcttcgta cgcggacgtc cgcgtcggcc gctaccggcg gcaggcggtc
     1081 ggtacgcgcg aacggcagat caccggcgtc agcgacagcg agtcgtacgg gctcggcgtg
     1141 cgcacgctcg tcaacgggtg ctgggggttt gccgcgacga gcgtgatgag ccgtgccggc
     1201 gtccgccaga ctgcgctcga ggcggtgacc ctgtcgaagg cggcccgcgc cgttcagaag
     1261 cggcgtgtgg agctcgcgcc gatcacgccc gtgacgggca cgtggatgac gccggtccgg
     1321 agggacccgt tgaccgtgcc ggtcgaagag atcgtcgcgc tgctgctctc caccaacgaa
     1381 gcggccttga aggtgaagaa cgtccggttc gtcagctcag gcctggcgct gctcagagaa
     1441 gtgaagacgc tcgtcacctc cgagggcacg aacgtcacgc agacgatgat tcgcgtcggg
     1501 ccgtcgttct cctgcacggc gacctctggc gccgacttcc agagctacga agccgagctc
     1561 gcgccgcgcg gtctcggctg ggagtacgtc gcgtcgctcg acatgccggg caacgccgag
     1621 cgatgggcct ccatcgccgc ggagaagctc gcggcgaaga gcgtcgaggc cggccagtac
     1681 gatctgatcc tcgagcccac gaatctctgg ctcacgatcc acgagtcgat cggccacgcg
     1741 acggagctcg atcgggcaat cggcgacgaa gccaactacg cgggcacgag cttcgtggcg
     1801 cccccggaga agatgctggg tcagcttcgg tacggtccgc cgatcatgaa cgtgcaggcg
     1861 gaccggacgc aggaaggctc gctgtcccgc gtcgcatggg acgacgaggg cgtgccggcg
     1921 gaccagtggc tgctcgtgga gcgcggcgtc ttcaaggact accagaccac gcgcgaacag
     1981 gtcgcgcgca tccagaagct gaccggcgtc accaggtcgc acggctgttc gtttgccgag
     2041 tcgtggaacg ccgtgcagtt ccagcgaatg ccgaacgtgt cgctgctgcc cggcgacagg
     2101 gacatcggcg tggacgacat cgtggccgac accagccgcg gcgtgctcat cagaaaccgg
     2161 ggctcctggt cgatcgatca ccagcgctac aacttccagt tctcgggaca ggccttctat
     2221 gaagtgcgca acggcaagat cgcgggaatg ctcaaggacg tggcatacca gagcagcacg
     2281 cccgtgttct ggaactcgat ggacctgatc ggcggcaagt cgtcgtactg gatgggtggc
     2341 gccttcaacg acggcaaggg cgagccgtcg cagagcaatt cggtgagcca cggctgcgtg
     2401 ccggcccggt tccgcaaggt gacgatcctc aacacggccc ggagcgcgtg atgcggaacg
     2461 caagcacaca ggcacccggg acccagagaa cggcggcacc gtccatcggc gtgcccctgc
     2521 tgtctcgtga agacgcgagg gcgctgaccg cgcgcgtgct cgcgctgagc accgccgacc
     2581 agacccgcgt gaccattcag agcggctgga gcggcaacac gcgcttcgcc gacggcggca
     2641 tcacgacgtc gggcgggacg accgacacga cgttgaccgt gtgggtcacc ttcggcaagc
     2701 gccgcgcctc gtcttcgacc aacgtgctcg acgaggcgtc gctgaagcgg acggttgatc
     2761 ttgcggcgcg cctcgcccgc ctcgcgccag aggatccaga gctcatgccg gagctcggtc
     2821 cgcagaacta cacgcccgtc gatgccttcg tcgcgggcac ggccgatctg gacgccgagg
     2881 ctcgtgcggc agcggtcgag cgcgccgtga gtgcggcgac cgcggcgggc aagccggcgg
     2941 gcgcgatctt cacggcgggg tacctcgagg ccaacgccca ggctgtcgcc gtcgcgacga
     3001 gcaacggctt gttcgcctac caccggacga cggacgcgga tttctcggtg accgcccgga
     3061 cgccggacgg caccggcagc ggctgggcgc acggcggttc gcgcgactgg agcgccgtgg
     3121 atccggcggc gatcggacgc ctggcggcgc agaaagccgt ggccagccgc aatcctcaag
     3181 cgatcgctcc cggtctctac accgcggttc tcgagccgca ggccgtgacc gatctcattc
     3241 cgctgctcgc cggtgcgctc aacgcgcgca gcgcggacga ggggcgcagc gccttctcga
     3301 agccgggcgg cggaacgcgg atcggcgagc g
//
LOCUS       DBNGSH010000862         5336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3671365, whole genome shotgun sequence.
ACCESSION   DBNGSH010000862 DBNGSH010000000
VERSION     DBNGSH010000862.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3671365"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC16CY_19810"
     CDS             complement(<1..245)
                     /locus_tag="AC16CY_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356107.1"
                     /translation="MRHTSLAALLILLSAPALAQSVTASVDEVVVHHDDAGRVRGRIV
                     DLGPQGRVLIAANQRLTLSVAGVQRIDTRRDSVWDGAV"
     gene            351..2657
                     /locus_tag="AC16CY_19815"
     CDS             351..2657
                     /locus_tag="AC16CY_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIR3356108.1"
                     /translation="MIRSVAVLGAGVMGAQIAAHMGNAGVPALLLDLTAEAAAEGLKR
                     ARAIKPDPFFTPDVWKLVTTASFDDGFTRIGEADWIIEAVVERLDVKQGLLARVDQFR
                     KPGSIVSSNTSGIPISALAEGRSEDFRRHWIGTHFFNPPRYLRLLEIIPTAETSPAVV
                     KSVSAFADHHLGKGVVVAKDSPNFIGNHLALMGVVPLIAMATSGEFTIEEIDAMTGPA
                     IGRPKSATFRTLDLAGLDILGHVIRNLHERLPDERERARFALPPLVERMLAKGLTGEK
                     AGQGFYKRVKGDGGDSEILTLDADTLEYRPRKSPKLPSLDAAAAITGTGERIKTLFEG
                     KDRVGQFLRRTLAPAIVYAAKVAPDIAHSPDDVDRVMRWGFGWELGPFETADAIGIGP
                     LLDVVRETDPDLLRDGVPAMWQPILTAGGNRMRGSQVPPAASDLQILRAARDRSRIVK
                     TNPGASLVDLGEGVLAVEFHSKMNAIGGDTIQMLQAGVREAERNFAALVVGNEAVHFS
                     AGANLMLVLLEAQEENWDEIDLMVRAFQQATMGLRYANVPVVVAPAGLALGGGCEIVL
                     HADRVQAAGETYMGLVEVGVGLIPAGGGTKEMTARAVEQMPPGSSDLLPSIQRAFETI
                     GFAKTSASGTDAVRLGYLRAVDGVTMNQERLIADAKNRALQRVREGYQPPARRTAIPV
                     GGDPVAATLKLGVHLAWRAGRISDHDKLIGRKLATIMTGGALPHPTTVSEQHLLDLER
                     EAFMSLVAEPKTRERIQYTLKTGKPLRN"
     gene            2770..3579
                     /locus_tag="AC16CY_19820"
     CDS             2770..3579
                     /locus_tag="AC16CY_19820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3356109.1"
                     /translation="MKIIDRGTGAPVVLVPGIQGRWEWLKPAVDALAARCRVITFSLA
                     DEPATGARFDADAGFQCYVEQIEAALDAVGVERATICGISYGGLVAAAFAARKPERTA
                     ALVLASALAPSWRPDWKARFFMRAPTLLSPLFCINSLRLLPEMAAAKQGVGSGLAFGA
                     RHVANVVTHMFSPRRMVRRMHLLEGLDLEHEVSDLHVPTLVITGEPRLDRVVPVRITR
                     DYLRLWPHADYATIERTGHIGSITRPDEFADLVFQFTERSSSLPAQRKRIV"
     gene            complement(3595..4011)
                     /locus_tag="AC16CY_19825"
     CDS             complement(3595..4011)
                     /locus_tag="AC16CY_19825"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3356110.1"
                     /translation="MTAYIDTSALLRLVLREPGALDDLRSADALVSSELIAVESARTI
                     DRLRLQGALTVEEAAARQRAVTEWLEAIDLVLVRAPVLKRASEPLPTPLGTLDGIHLA
                     TALIWRERMGALPTIATHDTALGLAARTFGFDVRGI"
     gene            complement(4008..4250)
                     /locus_tag="AC16CY_19830"
     CDS             complement(4008..4250)
                     /locus_tag="AC16CY_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3356111.1"
                     /translation="MTRVRIADLKSRLSEHLRSVRKGRTLTVLDRDTPVARIIPYGAE
                     PIEVRRATRRLRDLKLPPPPASPTDSLAILLDDRRR"
     gene            4368..5054
                     /locus_tag="AC16CY_19835"
     CDS             4368..5054
                     /locus_tag="AC16CY_19835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3356112.1"
                     /translation="MHGADYLCVRHRPDQGEAISIRELPGPAGRLEALLDEPDGAPRA
                     AVVFAHPLPTMGGTMHTKVVFQGAKALTRIGCVVLRFNFRGVGRSDGTWDNGRGELDD
                     YRAATDFLASKYPDLEMWAAGFSFGSYIAMTCGADDERMCALIGIAPPVDRYEFASVK
                     LSAKPKFIIHGERDEIISLKAVREFYGRLEEPKELVEIDRAGHLFDGQSQEVGDALED
                     LLGDFSCRKR"
     gene            5039..>5336
                     /locus_tag="AC16CY_19840"
     CDS             5039..>5336
                     /locus_tag="AC16CY_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3356113.1"
                     /translation="MQEAVIVSATRTAVGKAPNGSLRTVRPDDMAASVIGEALRRVPS
                     LRPADVDDVVMGCAMPEAEQGLNVARIASLRAGVPVEASAVTLNRFCASGLQAIA"
BASE COUNT          884 a         1759 c         1841 g          852 t
ORIGIN      
        1 acggcgccgt cccacaccga gtcgcgtcga gtgtcgatgc gctgcacgcc ggccactgaa
       61 agcgtcaggc gttgattggc ggcgatcaac acgcgcccct gcggaccgag gtcaacgatg
      121 cggccgcgga cgcggccggc gtcgtcgtgg tgaacgacga cttcgtccac ggacgctgtc
      181 accgactgcg ccaaggccgg cgcggacagc aggatcagca gcgcggcaag cgatgtgtga
      241 cgcatggcca cctccagggc catgatcagg gcgtctgccg gtggagtcct gagggattcc
      301 tgaaaagtac tgatacagcc ggaaccgtcg agccacgcta gactgatcgc gtgatccgtt
      361 ccgtcgccgt gctgggggcc ggcgtcatgg gcgcccagat tgcggcgcat atgggcaacg
      421 ccggtgtgcc ggccctgctg ctcgacctca cggcagaggc cgccgcggag ggcctgaagc
      481 gcgcgcgcgc catcaaaccc gatccgttct tcactccgga cgtctggaag ctcgtgacca
      541 cggccagctt cgacgacggt ttcacgcgca tcggcgaggc cgactggatc atcgaggcgg
      601 tcgtcgagcg gctcgacgtg aaacagggac tgcttgcgcg cgtcgatcaa ttccgcaagc
      661 cgggctcgat cgtcagctcc aacacgtcgg ggattccgat ctccgcgctt gccgagggcc
      721 gcagcgaaga cttcaggcgc cactggatcg gcacccactt cttcaacccg cctcgctacc
      781 tgcgcctgct cgagatcatt ccaaccgcgg agacgagccc cgccgtcgtc aagtccgtca
      841 gcgcgtttgc cgaccatcac ctgggcaagg gcgtcgtcgt cgcgaaggac tcgcccaact
      901 tcatcggcaa tcacctggcg ctcatgggcg tcgtgccgct catcgcgatg gccacctccg
      961 gcgagttcac gatcgaagag atcgacgcga tgacagggcc ggcgatcggg cgtccgaaga
     1021 gcgcgacgtt caggacgctc gatctggcgg gcctcgacat cctcggccac gtgatccgca
     1081 acctgcacga gcgtctgccg gacgaaagag agagggcgcg gttcgcattg ccgccgctcg
     1141 tcgagcggat gctggcgaaa gggctgacag gagagaaggc cggccagggc ttctacaagc
     1201 gcgtcaaggg cgacggcggc gactccgaaa tcctcacgct cgacgccgac acgctcgagt
     1261 accggccaag gaaatcgccg aagctccctt cgctcgacgc agccgcagcc atcacaggca
     1321 cgggcgagcg aatcaagacg ctgttcgagg gcaaggaccg cgtcgggcag ttcctccgcc
     1381 gcacgctggc gccagccatc gtctatgccg cgaaggtggc acccgacatc gcccactccc
     1441 ccgacgatgt ggaccgcgtg atgcgctggg gattcggctg ggagcttggg ccgttcgaga
     1501 cggcggacgc catcgggatc ggtccgctcc tcgacgtcgt ccgcgaaacg gaccctgacc
     1561 tcctgcgcga cggcgtcccc gccatgtggc agccgatcct gactgccggc gggaaccgga
     1621 tgcgaggcag tcaggtgcca ccggccgcat ccgaccttca gattctgcgc gccgcgcggg
     1681 accgatcacg gatcgtgaag acgaacccgg gggccagcct cgtcgatctc ggcgagggcg
     1741 tgctggccgt cgagttccac tcgaagatga acgccatcgg cggcgacacg attcagatgc
     1801 tgcaggccgg cgttcgtgaa gcggagcgga acttcgcggc cctggtcgtc ggaaacgagg
     1861 cggtgcactt ctccgccggc gccaacctga tgctcgtgct cctcgaggcg caggaagaga
     1921 actgggacga gatcgacctg atggtccgcg cgttccagca ggccacgatg gggctgcgct
     1981 acgcgaacgt gccggtggtc gtcgcgcccg cggggctggc gctcggcggc ggctgcgaga
     2041 tcgtgctcca cgcggatcgc gtgcaggcgg cgggtgaaac ctacatgggg ctcgtcgaag
     2101 ttggcgtggg cctgattccc gctggcggcg gtacgaagga aatgacggcg cgcgccgtcg
     2161 agcagatgcc gccgggcagc agcgacctcc tgccgtcgat ccagcgcgcg ttcgaaacga
     2221 ttgggttcgc gaagacgtcc gccagcggta ccgacgcggt ccgcctcgga tatctccggg
     2281 ccgttgacgg cgtgacgatg aaccaggaac ggctgattgc cgacgccaag aatcgagcgc
     2341 tgcagcgggt gcgcgagggt tatcaaccgc ccgcgcggcg gacggcgatc ccggtcggcg
     2401 gcgacccggt ggccgcaacg ctgaagctgg gagtccacct tgcgtggagg gccggccgga
     2461 tcagcgatca cgacaagttg atcgggcgca agctggcgac gatcatgact ggaggggcgc
     2521 tcccccaccc gacgaccgtg tcggagcagc acctgctcga cctcgagcgt gaggcgttca
     2581 tgagcctggt ggcggagccg aagactcgcg agaggattca gtacacgctg aaaacaggca
     2641 agcctttacg aaactgagga cctgcgtgat cacgagccac ggagacacgg aggcatggag
     2701 acgaattcct aaagaagatt cctccgtgtc tccgtgcctc cgtggttcgt gtgattcgtg
     2761 gcaggggcga tgaagatcat cgaccgagga acgggcgcgc cggtcgtgct ggtgccgggg
     2821 attcagggcc gctgggaatg gctgaagccg gccgtggacg cgctcgccgc gcgctgccgc
     2881 gtgatcacgt tttcgctggc cgacgagccg gcgacggggg cccgcttcga tgcagacgcc
     2941 ggattccagt gctatgtgga gcagatcgaa gccgcgctcg atgcagtggg cgtggaacgg
     3001 gcgaccatct gcggcatctc gtacggcggc ctcgtcgcgg cggcgtttgc cgcacggaag
     3061 ccggagcgca ccgcggcgct cgtgctggcc tccgcgctcg cgccgtcctg gaggccggac
     3121 tggaaagcca ggttcttcat gcgggccccg actcttctct ctccgctgtt ctgcatcaac
     3181 tcgctgaggc tcttgcccga gatggcggct gcgaaacagg gcgtcggatc cggtctcgcg
     3241 ttcggcgccc gtcacgtggc caacgtggtc actcacatgt tctcgccccg gcgcatggtg
     3301 cggcggatgc acctgctcga ggggctcgat ctcgagcacg aagtgtccga cctgcacgtg
     3361 ccgacgctcg tgatcaccgg tgagccgagg ctcgatcgcg tcgtccccgt gcgcatcacg
     3421 cgcgattacc tgcggctctg gccgcacgcc gactacgcca cgatcgagcg gaccggccac
     3481 atcggctcga tcacccggcc cgacgagttc gccgacctcg tcttccagtt caccgaacga
     3541 tcgtcgagtc tccctgccca gcggaagcga atcgtctaaa cacagccttc tctctcagat
     3601 tccgcgaacg tcgaatccga aggtccgtgc ggcgaggccg agagcagtgt cgtgtgttgc
     3661 gatggtcggc aaggccccca tgcgctcacg ccagatcagc gcggtcgcca ggtggatccc
     3721 atcgagcgtt ccgagcggcg tcggcagcgg ctcgctggca cgcttcagca cgggtgcacg
     3781 caccagcacg aggtcgatcg cctcgagcca ctccgtgacc gctcgctggc gcgctgccgc
     3841 ttcctccacc gtcagtgcgc cctgaagtcg cagtcggtcg atcgtccgcg cgctttcgac
     3901 ggcgatcagt tcgctcgaca ccagcgcgtc ggcggaccgg agatcgtcga gggcgcccgg
     3961 ctcgcgcagc acgagccgga gcagcgccga tgtgtcgatg taggcggtca ccgccgccgg
     4021 tcgtcgagca ggatggccag actgtcggtc ggcgatgcgg gtggcggcgg gagcttcagg
     4081 tcgcgaagtc ggcgagtggc ccgccgaacc tcgatcggct cggcgccata ggggatgatc
     4141 cgtgcgacgg gcgtgtcgcg atcgagcacg gtcagcgtgc gtcccttccg gaccgatcgg
     4201 agatgctcgc tcagacgcga cttcagatcg gcgattcgca ccctcgtcat gaccatatta
     4261 tagtcacaag tggtcacgct gggacggcgc ggcgccgggc tgcatcgtct atgctgggta
     4321 ctgttccatc gatcgcagag tgcccgtaat tccgggtcgg attcgtagtg cacggcgctg
     4381 attatctctg cgtccgccat cgaccagatc aaggagaggc cataagcatc cgcgagcttc
     4441 cgggtccggc gggacggctc gaagcgctgc tcgacgagcc ggacggggcg ccgcgcgcgg
     4501 cggtcgtgtt cgcccacccg ctgccgacca tgggcggcac gatgcacacc aaggtcgtct
     4561 ttcagggcgc caaggcgctc acgcgcatcg gctgcgtcgt gctgaggttc aacttccgcg
     4621 gtgtgggccg cagcgacggc acgtgggaca acggacgagg cgagctggac gattaccggg
     4681 ccgccaccga tttcctcgcg tcgaagtatc ctgatctgga gatgtgggcc gcggggttct
     4741 ctttcggttc gtacatcgcg atgacgtgcg gcgccgacga cgagcggatg tgcgcgctca
     4801 tcggcatcgc gccacccgtt gaccggtacg agttcgcctc ggtgaagctc tccgccaagc
     4861 cgaagttcat catccacggc gagcgcgacg agatcatctc gctcaaggcc gtccgcgagt
     4921 tctacggccg gctcgaggag ccgaaagagc tcgtggaaat cgaccgcgcc ggccacctct
     4981 tcgacgggca gtcgcaggaa gtgggcgacg cgctggaaga cttgctcggg gatttctcat
     5041 gcaggaagcg gtaatcgtct ccgccacgcg caccgccgtc ggcaaggcgc ctaacggctc
     5101 gctcaggacc gtccgcccgg acgacatggc ggcgtccgtg atcggcgagg cgctgcgccg
     5161 cgtgccgtcg ctgcgcccgg ccgacgtgga cgacgtggtg atggggtgtg cgatgccgga
     5221 ggcggagcag gggttgaacg tcgcccgcat cgcgagcctg cgcgcgggcg tgccggtcga
     5281 ggcatccgcc gtcacgctca acaggttctg cgcgtcgggg ctccaggcga tcgcct
//
LOCUS       DBNGSH010000863         2491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4010845, whole genome shotgun sequence.
ACCESSION   DBNGSH010000863 DBNGSH010000000
VERSION     DBNGSH010000863.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4010845"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC16CY_19845"
     CDS             <1..384
                     /locus_tag="AC16CY_19845"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3356114.1"
                     /translation="HHSNPLYFDMSTAITIEGEVLRLEWINPHVLLFLQSKNDKGELE
                     TWILQGASLNNANRQVGLKERLQPGTRISARVMPPRNPLFVNDAQVVLLTKPDDARQS
                     SRIVGGGQIRFSNGDVMNLGGGPKF"
     gene            439..1248
                     /locus_tag="AC16CY_19850"
     CDS             439..1248
                     /locus_tag="AC16CY_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356115.1"
                     /translation="MPWNSLTFMKQLDACGNRYEAENAWYTKKQWGEQVKYMLNAWNL
                     YRSASTNALGMLLLGDGKIRKDAALLGNFGITSVGGAWDPEEQKLVKELEAKRGQMRT
                     APGLQDAPKVQGQGSVLSDIGWTPLMNDAYILGGTHGLHEFHLALEDAKDPNKPELKT
                     LAHDFALTRETDPVKRWQSFFQSKLDMFWDPRWNSPRVLSRELIGLDMCGYEPQFSLE
                     QLSFASRGVDNATFSDYLDALISSGYRTKDKKALMDVLSQFLFKKPGLLSA"
     gene            1262..>2491
                     /locus_tag="AC16CY_19855"
     CDS             1262..>2491
                     /locus_tag="AC16CY_19855"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR3356116.1"
                     /translation="MRLATLASALVCAISLLVDAQAPAPIDAAMQAFWSADGGGDREA
                     AGRRVAAGGAAFDDVWRRLRTGRAYGARKTGRIELPSTDAGVRLDNVAEIPAEYDPAR
                     ASPVRVSLHGGVGREAPGAGDPPARPLANRIPSAGEIVIHPRAWSASQWWHAGQVDNI
                     RRLIERVKRDYNVDESRMYVTGISDGGTGVYFLAMRDATPWAACMPMNGHPSVIANPD
                     TGADGQLYAGNLFNCPLRIVNGGRDRLYPAASVAPIVEMFKRTKATLEFQVYPDADHD
                     TSWWPQERPRFEAFLAAHRRAAHPSSVSWETERTDRYNRFRWLVIDRLGRRASDVALE
                     DVNTSSRAAIMERPLFERRRPSGRVDVERRGNSFEARTRGVQAFTLLLSPDVVDFGKP
                     VRVTVNGRTVHDGVVTKD"
BASE COUNT          428 a          829 c          865 g          369 t
ORIGIN      
        1 catcactcca atccgctcta cttcgacatg tccacggcca tcacgatcga aggcgaggtc
       61 ctgcgcctgg agtggatcaa cccgcacgtc ctcctgtttc tccagtcgaa gaacgacaag
      121 ggcgagctcg agacgtggat actccagggc gcttcgctca acaacgcgaa tcgccaggtg
      181 ggcctgaagg agcggctgca gccgggcacc cggatctcgg cccgcgtgat gccgccccgc
      241 aacccgctgt tcgtgaacga cgcgcaggtg gtgctgctga cgaagcccga cgatgccagg
      301 cagtcctcgc gcatcgtggg agggggacag atccggttct ccaacggcga cgtcatgaac
      361 cttggcggtg gaccgaagtt ctgaacgagc ggggccacgg gctcagggat tccggctatc
      421 atccccccgg agagccgcat gccctggaac agcctgacct tcatgaagca gctcgacgcc
      481 tgcgggaacc gctacgaagc cgagaacgcc tggtacacga agaaacagtg gggtgagcag
      541 gtcaagtaca tgctcaatgc gtggaacctc taccggagtg cgtccaccaa tgcgctgggc
      601 atgctgctgc tcggcgacgg caagattcgg aaggacgcgg cgctgctcgg caacttcggg
      661 atcacgtcgg tcgggggcgc atgggatccc gaggaacaga agctcgtcaa ggagctcgag
      721 gccaagcgcg gccagatgcg gaccgccccg gggctgcaag acgcgcccaa ggtgcagggg
      781 cagggaagcg tgctcagcga catcggctgg acgccgttga tgaacgacgc ctacatcctg
      841 ggcggcacgc acggcctgca cgagttccac ctggcgctcg aggacgccaa ggaccccaac
      901 aagcccgaac tgaagacgct ggcccacgac ttcgccctga cgcgcgagac ggacccggtg
      961 aaacggtggc agtcgttctt ccagtcgaag ctcgacatgt tctgggaccc gaggtggaac
     1021 tccccgcgcg tgctgtcgcg cgagctgatc ggcctggaca tgtgcggata cgagccgcaa
     1081 ttctccctgg agcagctctc gttcgcctcg cggggagtgg acaacgccac cttcagcgac
     1141 tacctcgacg cgctcatctc gagcggctac cggaccaagg acaaaaaggc gctgatggac
     1201 gtgctctcgc agttcctgtt caagaagccg gggctgctgt ccgcgtaaga tcggcgtcac
     1261 catgcggctc gcgacgctcg ccagcgcgct cgtttgcgcc atctctctcc tcgtcgacgc
     1321 gcaggcgccg gctccgatcg acgctgcgat gcaggcgttc tggagcgccg acggtggcgg
     1381 cgacagggag gcggccggcc gaagggtcgc ggccggcggt gcggcgttcg acgatgtgtg
     1441 gaggcggctg cggaccggcc gggcgtacgg cgccaggaag accgggcgca tcgagctgcc
     1501 ctcgaccgac gctggcgtgc ggctcgacaa cgtggccgag atccccgccg agtacgaccc
     1561 cgcgcgcgcg tcgcccgtgc gcgtgagcct gcacggcggg gtcgggcgcg aggcgcccgg
     1621 tgccggcgat ccaccggcac ggccgctcgc caacaggatt ccgagcgcgg gcgagatcgt
     1681 catccatccg cgcgcgtggt cggcgtcgca gtggtggcac gccggccagg tggacaacat
     1741 ccggcggctc atcgagcgcg tcaagcgcga ctacaacgtg gacgagtcgc ggatgtacgt
     1801 gacggggatc tccgacgggg gcaccggcgt ctatttcctc gcgatgcgcg acgcgacgcc
     1861 gtgggccgcg tgcatgccga tgaacggcca cccgtcggtc atcgccaacc ccgacacggg
     1921 cgccgacggc cagctctatg ccgggaatct tttcaactgc cccctgcgca tcgtcaacgg
     1981 cggccgcgat cggctgtatc cggccgcgtc ggtggcgccg attgtcgaga tgttcaagcg
     2041 gacgaaggcg acgctcgagt tccaggtgta cccggacgcc gatcacgaca cgagctggtg
     2101 gccgcaggag cgcccgcgct tcgaggcgtt cctcgcggcg catcgccgcg cggcgcaccc
     2161 gtcgagcgtc tcgtgggaga ccgagcggac cgatcgctac aaccgtttcc ggtggctcgt
     2221 gatcgatcgc ctcggccggc gtgcgtccga cgtcgcgctc gaggacgtga acacctcttc
     2281 gcgggcggcg atcatggagc ggccgctttt cgagcgacgc cggccatccg gccgcgtgga
     2341 cgtggagcgc cgcggcaaca gcttcgaggc gaggacgcgc ggcgtccagg cgttcacgct
     2401 gctgctctcg ccggacgtcg tcgatttcgg aaagccggtc cgcgtgaccg tcaacggcag
     2461 gacggtgcac gacggcgtcg tgacgaagga c
//
LOCUS       DBNGSH010000864         2515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1803593, whole genome shotgun sequence.
ACCESSION   DBNGSH010000864 DBNGSH010000000
VERSION     DBNGSH010000864.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1803593"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="AC16CY_19860"
     CDS             <1..458
                     /locus_tag="AC16CY_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356117.1"
                     /translation="ESRNELRLPVSFQLRGPAVVVRRVRVVTESGGEMTTWDGTFEAD
                     DEPVAEFAPADGEIRKHRKPLRPFELSAQSSFVAKIFDFVAENPEPLGTGSFAIQLQV
                     QAGSAEWSTVQKGSFTVPADFTLTGRNASGAEFHRYDRWQVFPLKIASR"
     gene            complement(465..>2515)
                     /locus_tag="AC16CY_19865"
     CDS             complement(465..>2515)
                     /locus_tag="AC16CY_19865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3356118.1"
                     /translation="VADAAGVFEPNIWIKINADDTVRIMLTMLEMGQGVMTSMPMLVA
                     EELDFDWTKIKTEWAPADAKYGNPNFGGQQLTAGSNSVRGMWKVLRESGATARAMLVS
                     AAAQTWGVAESACTTDKGEVIHQASGRRLKYGALVDKAAALPVPKSVALKNPKAFKVL
                     GQSLARLDVPEKVNGTAVFGIDVKLPGLLTARVVRCPVFGGKVARLNADKAKAVPGVS
                     HVVQISTGVAVVADNYWSASRGAQALQITWDEGPLATLTSAEIMKRYKTLAQQAGKVA
                     RNDGNADATLAASPRAFERTYEAPFLAHATMEPMNCTADVRADGCDVYVPTQGQTASH
                     LAAVAASGLPPDKVRIHTTYMGGGFGRRGEGDFVTDAVETSKAVGKPVKVVWSREDDM
                     QHDYYRPVSYARMWGAIDPSGKPTVFKQHIVQQSLMKRIGGLPPNGVDFISVDGSANL
                     PYAIPNIRVEYTETDPGIPFGFWRSVGASFQGFVVEAFIDEMATTAGKDPYEFRRELL
                     GKSPRHRAVLELAAEKAGWGKPLPAGRARGIAVMDAFGSYLAQVTEVSVDARGSVRVH
                     KIVCTVDTGWVINPDTIKAQMEGGIVYGLTAALRGEITIKGGRVEQRHFGDYQMLRHN
                     EMPEIEVHIVPSTEDPGGIGEPSTALAAGSLVNAVFAATGRRIYSLPIRPEQLRGGTA
                     "
BASE COUNT          368 a          886 c          813 g          448 t
ORIGIN      
        1 ccgaatcgcg gaacgagctg cggcttccgg tctcgtttca gctccgcgga cccgctgtcg
       61 tcgtccgccg cgtccgcgtg gtcacggagt cgggcggcga gatgacgacc tgggatggga
      121 ctttcgaagc agacgacgag ccggtggccg agttcgctcc ggctgacggc gagattcgca
      181 agcaccggaa gccgctgcgg ccgttcgagc tgtcggcaca gagctcgttc gtcgcgaaga
      241 tcttcgactt cgtcgcggag aatccggagc cactcggaac cgggagcttc gccatacagc
      301 ttcaggtgca agcgggctct gccgagtggt ccaccgtgca gaaggggtcg tttacggtgc
      361 ccgccgactt cacgctcacc ggacgcaacg cgtcaggagc cgaattccat cggtacgatc
      421 gatggcaggt gttccccctg aagatcgcgt cgcggtgaac tcgatcaggc cgttccgccc
      481 cgcagctgct ccggccgaat cggcagcgag tagatgcgcc tcccggtcgc ggcgaagacg
      541 gcgttgacga gcgatccggc cgcgagcgcg gtgctcggtt cgccaatccc tccgggatcc
      601 tccgtgctcg gcacgatatg gacctcgatc tccggcatct cgttgtgccg cagcatctgg
      661 tagtcgccga agtgcctctg ctcgacgcgg ccgcccttga tcgtgatctc ccccctcagc
      721 gcggcggtca acccgtagac gatcccgcct tccatctgcg ccttgatcgt gtcggggttg
      781 atcacccacc cggtgtccac ggtgcagaca atcttgtgca cgcgcacgct gccacgcgcg
      841 tccaccgaca cttcggtcac ctgcgcgagg tagctgccga acgcgtccat caccgcgatg
      901 ccgcgcgcgc gtcccgccgg cagaggcttg ccccaaccgg ccttttcggc cgcgagctcg
      961 agcacggctc gatggcgcgg cgacttgccg agcagctcgc gccggaactc gtacggatcc
     1021 ttcccggccg tcgtcgccat ctcgtcgatg aacgcctcga cgacgaagcc ctggaacgag
     1081 gcgccgaccg atcgccagaa cccgaacggg atgcccggat cggtttccgt gtactcgacg
     1141 cggatgttcg ggatggcgta cgggaggttg gctgagccgt caaccgagat gaagtccacg
     1201 ccgttgggcg gaagcccgcc gatgcgcttc atcagcgact gctgcacgat gtgctgcttg
     1261 aagaccgtcg gcttgcccga cgggtcgatg gcgccccaca tgcgcgcgta gctgacggga
     1321 cgatagtagt cgtgctgcat gtcgtcctcg cggctccaca cgaccttgac cggcttcccc
     1381 accgctttcg acgtctcgac ggcgtcggtg acgaaatctc cctcgccgcg ccggccgaaa
     1441 ccgcctccca tgtaggtggt gtgaatcctg accttgtcgg gaggcagccc ggacgcggca
     1501 accgcggcca ggtgcgacgc cgtctgtccc tgggttggca cgtacacgtc acacccgtcg
     1561 gcgcgcacgt cggccgtaca gttcatcggc tccatcgtgg cgtgcgccag gaagggagcc
     1621 tcgtaggtcc gctcgaaggc acgaggcgac gcggccagcg tggcgtcggc gttgccgtcg
     1681 ttgcgagcca ccttccccgc ctgctgcgcg agggtcttgt agcgcttcat gatctcggcg
     1741 ctcgtcagcg tggccagcgg gccttcgtcc cacgtgatct ggagcgcctg tgctccgcgc
     1801 gacgcggacc agtagttgtc ggcgaccacc gcgacgccgg tgctgatctg cacgacgtgc
     1861 gagacgcccg ggacggcctt cgccttgtcg gcgttgagcc tggcgacctt gccgccgaag
     1921 accgggcagc gcaccacgcg cgcggtcaga agtcctggca gcttgacgtc gatgccgaac
     1981 accgccgtgc cgttgacctt ctccggcacg tcgaggcgcg cgagcgactg cccgagcacc
     2041 ttgaaggcct tcggattctt gagcgccacg ctcttcggta cgggcagcgc ggccgccttg
     2101 tccacgagcg cgccgtactt cagccgccgt ccgctcgcct ggtgaatcac ttcgcccttg
     2161 tcggtcgtgc acgcgctctc ggcaacgccc cacgtctgcg cggcggcgga tacgagcatc
     2221 gcgcgcgcgg tcgcgcccga ttcgcgcagc accttccaca tcccgcgcac gctgttgctg
     2281 ccggcggtga gctgctggcc cccgaagttc gggttgccgt acttcgcatc ggccggggcc
     2341 cactcggtct tgatcttcgt ccagtcgaag tcgagctcct cggccacgag catcggcatc
     2401 gacgtcatca cgccctgccc catctcgagc atcgtcagca tgatgcgcac cgtgtcgtcg
     2461 gcgttgatct tgatccagat gttgggctcg aacacgcccg ccgcgtcggc gacgc
//
LOCUS       DBNGSH010000865         2025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2314257, whole genome shotgun sequence.
ACCESSION   DBNGSH010000865 DBNGSH010000000
VERSION     DBNGSH010000865.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2314257"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="AC16CY_19870"
     CDS             complement(<1..681)
                     /locus_tag="AC16CY_19870"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356119.1"
                     /translation="MPARSTVAAVLLWVLSCTPLHAQTTVAELNEAGWQMLDQGDGAR
                     ASKLFAEALALRPDEPVLLYGAGVAAHLQGRASDARRHVRRALEQNPRFTPASLLLGE
                     FAYRDGDLTLAIKTYESALALSPGNRDLSSRLQQWRQEADVHSSFVERRQDRFRVMFE
                     GHVEQQLATRATAMLTDAFWRIGGVLGAYPSDVVGVTLYTARQFRDITRAPEWSGGIY
                     DGRIRVPAA"
     gene            complement(802..>2025)
                     /locus_tag="AC16CY_19875"
     CDS             complement(802..>2025)
                     /locus_tag="AC16CY_19875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR3356120.1"
                     /translation="EPKIEELLKEGQEIIVQVAKEPLGTKGARLTSHATMPGRFLVFM
                     PTVDHVGVSRKIDSREERSRLRGIVREFREQTGFTGGLIIRTAAAGRPKEDIVSDLSF
                     FHRVWTEIRQKAETSRAPAVIYQEQSLVAKLLRDLLTDDYSAIRIDNETEYRRVLELV
                     ERMMPSLGPKVKRYEREYPIFEEYGVQSEIDRALRSKVWLKSGGSIVINQTEALVAID
                     VNTGRYVGKKTAGRLEDTILKTNLEAVKEIVRQIRLRDLGGIIVLDFIDMEEKKNRQK
                     VFQAVEQELRKDRSPSKALQVSDFGLVIITRKRVKQSLERVLTEPCPYCAGSAVIKSA
                     STICYEILSEVKKISADLNGQTLVLRVNPDIARALRDEERAVFKDLKQALNRDIALRP
                     DAQLHHEQFDLMTVG"
BASE COUNT          305 a          690 c          666 g          364 t
ORIGIN      
        1 cgccgccggg acgcgaatcc gtccgtcgta gatgccaccc gaccactcgg gggcgcgcgt
       61 gatgtcgcga aactgcctgg cggtgtagag cgtcacgccg accacgtcgg acggataggc
      121 gccgagcacg ccgccgattc gccagaaggc gtcggtgagc atcgccgtgg ctcgagtcgc
      181 caactgctgc tcgacgtgcc cttcgaacat gacccggaag cgatcctggc ggcgttccac
      241 gaagctgctg tggacgtcgg cctcctgccg ccactgctgc agcctcgacg agaggtcccg
      301 gttgcccggc gagagcgcca gcgccgattc gtaggtcttg atcgcgaggg tcaggtctcc
      361 gtcgcggtac gcgaactccc cgagcagcag cgacgcgggg gtgaagcggg gattctgttc
      421 cagcgcccgc cgcacgtggc gccgcgcgtc gctggcgcgc ccctgcaggt gcgcggcgac
      481 gcccgcgccg tagagcagca cgggctcgtc cggccgcagc gcgagggcct cggcaaagag
      541 cttcgacgcc cgcgccccgt cgccctggtc gagcatctgc cagccggcct cgttcaactc
      601 ggcaacggtc gtttgcgcgt gaaggggcgt gcagctgagg acccacagca acacggcggc
      661 aacggtcgat cgcgcaggca cgcacggagc acctgccaac tccagaccag cgccggaccg
      721 tgcagtcgcg ccgccagcta cggaaggact cgacggcgga ttacagtttg gatcgcgcta
      781 tttcgttttc gctccctcgc tctagccgac tgtcatcagg tcgaactgct cgtggtggag
      841 ctgcgcgtcc ggcctgaggg cgatgtcccg gttcagcgcc tgcttcaggt ccttgaagac
      901 ggcgcgctcc tcgtcgcgaa gggcgcgcgc gatatcgggg ttcacgcgca gcacgagcgt
      961 ctgcccgttg aggtccgcgc tgatcttctt cacctccgac agaatctcgt agcagatggt
     1021 ggacgccgac ttgatcaccg cgctaccggc gcagtagggg cacggttcgg tcagcacgcg
     1081 ctccaggctc tgcttgacgc gcttcctggt gatgatcacg agcccgaagt cggatacctg
     1141 cagcgccttg gacggcgacc gatccttccg cagctcctgc tccacggcct ggaagacctt
     1201 ctgccggttc ttcttctctt ccatgtcgat gaagtcgagc acgatgatgc cgccgagatc
     1261 gcgcaggcga atctggcgga cgatctcctt cacggcctcg agattggtct tgaggatcgt
     1321 gtcctccagg cgcccggcgg tcttcttgcc gacgtagcgg ccggtgttca cgtcgatggc
     1381 gacgagcgcc tccgtctggt tgatcacgat cgagccgccg gacttgagcc agaccttgct
     1441 ccgcagcgcg cggtcgatct cggactgcac gccgtactcc tcgaagatgg ggtactcgcg
     1501 ctcgtaccgc ttcaccttcg ggccgagcga cggcatcatg cgctcgacga gctcgagcac
     1561 cctgcggtac tcggtctcgt tgtcgatgcg aatcgccgag tagtcgtcgg tcagcaggtc
     1621 gcggaggagc ttggccacga ggctctgctc ctggtagatc acggccggcg cgcgcgacgt
     1681 ctcggccttc tgccgtattt cggtccacac gcgatggaag aaggacaggt cggagacgat
     1741 gtcttccttc ggccgccccg cggcagccgt gcggatgatc agcccgccgg tgaagccggt
     1801 ctgctcgcgg aactcgcgaa cgatgcccct cagccgcgag cgctcttcgc gtgagtcgat
     1861 cttgcgcgac acgccgacgt ggtccaccgt cggcatgaac accaggaagc gcccgggcat
     1921 cgtcgcgtgg gacgtcaggc gcgcgccctt ggtccccagc ggttccttcg ccacctggac
     1981 gatgatttcc tggccttcct tgaggagctc ttcgatcttg ggctc
//
LOCUS       DBNGSH010000866         2969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3163276, whole genome shotgun sequence.
ACCESSION   DBNGSH010000866 DBNGSH010000000
VERSION     DBNGSH010000866.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3163276"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..753)
                     /locus_tag="AC16CY_19880"
     CDS             complement(40..753)
                     /locus_tag="AC16CY_19880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3356121.1"
                     /translation="MVGESGPTVVAVLTRAPSSGGKSRLFAALGRPSDSSLLAALLLD
                     TLDGAALDGTSRVVVVEPAEACDDVRAIVPGDVSVIAQAGGTLGDRMRETMASLLAGG
                     AGAVILIGSDLPDITPQMIGLAVQHLSRDPRSLVLGPSADGGYYLIGATGVPDVFDGI
                     EWGGPRVLAQTCAAAADRGLRVHLLEPLRDVDSREDLLLVAAPRTRAWVEKNRTSCPA
                     SLGAEARSAKAAAGPTPHS"
     gene            complement(714..1331)
                     /locus_tag="AC16CY_19885"
     CDS             complement(714..1331)
                     /locus_tag="AC16CY_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR3356122.1"
                     /translation="MKPIVVVKVLCFAAALIPAAALARGLYAALVLGDLNALTANPGD
                     YITDQTGTWTIAFLTIALAVTPLRRLTGWNDVVKLRRMLGLFAFFYATLHMLTWVVFV
                     NYFDVPSMIVDVAKRPFITVGMATFLILLTLAVTSNRFAIRKLGRRWQTLHRLVYVAG
                     ILAVVHFWWLVKADITLPRRWAFAIGSLLLFRVWWANRGRLSLRS"
     gene            complement(1328..2278)
                     /gene="msrP"
                     /locus_tag="AC16CY_19890"
     CDS             complement(1328..2278)
                     /gene="msrP"
                     /locus_tag="AC16CY_19890"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR3356123.1"
                     /translation="MLIKKAPGIRSSEITPKNVYLGRREFIQASAAALVAAGLPVPDA
                     AAQGGLAKLPNIRKSPLSTTETPSLYDHVTSYNNFYEFAPGAGDGPRNNSKNFKPRPW
                     KIKIDGLVAKPGDYDIDEFIKPYALEERIYRMRCVEAWSFVIPWVGIPMADVIKRVEP
                     TPKAQYVEMTTLHDVARFPMQRTDVLQWPYLEGLRLDEAQNPLTLLAVGLYGEVLPAQ
                     NGAPIRLVVPWKYGFKGVKSIVRIRFVENQPRNTWQTMAPHEYGFYANVNPTVDHPRW
                     SQARERRLPSFVPNHKTEMFNGYTEQVAHMYKGMDLRKNY"
     gene            2371..>2969
                     /locus_tag="AC16CY_19895"
     CDS             2371..>2969
                     /locus_tag="AC16CY_19895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3356124.1"
                     /translation="MIIPVLADGRAAGQLVTQLRVDPRVEVIVSSPDSGLESLEHGRP
                     DLRVVQTATGRAVQMNSGAGLARGDWLLFLHADSRVPDGWLDALEKAAAAGTVGGWFR
                     FALDDPSWQARLIERGVALRIKLFRLPYGDQGLFVRRQTFAAIGGYRDMPLMEDVDFV
                     RRLTRAGEVAEIPLPIATSARRWKRDGWFRRSARNLTLLG"
BASE COUNT          496 a          962 c         1006 g          505 t
ORIGIN      
        1 ttggcatggt cgtcgatcgc ttcggcgtgc cgtgggtgat caactgtgag gggtaggtcc
       61 ggctgccgcc ttcgcgctgc gcgcttcggc gcccaaggaa gccggacacg acgtacgatt
      121 cttctcgacc cacgctcgcg ttcgtggcgc cgcgacgagc aacaagtctt ctctcgaatc
      181 tacgtcgcgc agcggctcga gcagatggac gcgcagtccc cgatcggccg ccgccgcgca
      241 cgtctgagcc aggactcgcg ggccgcccca ctcgatcccg tcgaacacgt ctggcacgcc
      301 cgtagctccg atcaagtagt acccgccgtc ggcggacgga ccgagaacca gcgagcgggg
      361 atcgcgcgag aggtgttgga cggccaggcc gatcatttgc ggcgtgatgt ccggcagatc
      421 cgatccaatc aggatcacgg cccccgcgcc tcccgccagc agcgaggcca tggtctcgcg
      481 catacggtcg ccgagcgtgc cgccggcttg cgcaatcacg ctgacgtcgc cgggcacgat
      541 cgcgcgaacg tcgtcgcacg cctcggccgg ctcgacgaca acgacgcgtg acgtcccgtc
      601 gagggcggcg ccatccagcg tgtcgagcag aagcgcggca agcagcgacg agtcggaagg
      661 cctgccgagg gcggcaaaca gccggctctt gccgccgctc gagggcgcgc gcgtcaggac
      721 cgcaacgaca gtcggccccg attcgcccac catacgcgga acagcagcag cgagccgatc
      781 gcgaacgccc agcggcgcgg cagcgtgatg tcggccttga cgagccacca gaaatgcacg
      841 accgcgagga tcccggcgac gtacacgaga cggtgcagcg tctgccagcg gcggccgagc
      901 ttccggatcg cgaagcggtt ggacgtgacc gcgagtgtca gcagaatgag aaacgtggcc
      961 atccccaccg tgatgaacgg ccgcttcgcg acgtcgacga tcatggacgg gacgtcgaag
     1021 tagttgacga agacgaccca cgtcagcatg tgcagcgtgg cgtagaagaa cgcgaagagg
     1081 ccgagcatcc gccggagctt gacgacgtcg ttccatcccg tcaggcgccg gagcggcgtc
     1141 acggcgagcg cgatcgtcag aaacgcgatc gtccaggtgc cggtctggtc ggtgatgtag
     1201 tcgcccgggt tggcggtgag ggcgttgagg tcgcctaaaa cgagtgccgc gtagaggccc
     1261 ctggcaagcg ccgcggcggg gatgagcgcc gcggcgaagc acaggacctt gacgacgacg
     1321 atcggcttca ataattcttg cgcaggtcca tgcccttgta catgtgggcg acctgctcgg
     1381 tgtagccgtt gaacatctcc gtcttgtggt tggggacgaa gctcggcagc cggcgctccc
     1441 gcgcctggct ccaccgagga tggtccaccg tggggttcac gttcgcgtag aagccgtact
     1501 cgtgcggcgc catggtctgc cacgtgttcc tcggctggtt ctcgacgaac cggattctca
     1561 cgatcgactt cacgcccttg aagccgtact tccagggcac gacgagccgg atcggcgcgc
     1621 cgttctgcgc cggcagcacc tcgccgtaca atccgaccgc cagcagcgtc aacgggttct
     1681 gcgcctcgtc caggcggagg ccttcgaggt acggccactg gagcacgtcg gtgcgctgca
     1741 tcgggaatcg agccacatca tgcagcgtcg tcatctcgac gtactgtgcc ttgggggtcg
     1801 gctcaacacg cttgatcacg tcggccatcg gaatgccgac ccacggaatg acgaacgacc
     1861 acgcctcgac gcatcgcatc cggtagatgc gttcctcgag tgcatagggc ttgatgaact
     1921 cgtcgatgtc gtagtcgccc ggcttggcca ccagcccatc gatcttgatc ttccacgggc
     1981 ggggcttgaa gttcttcgag ttgttcctcg ggccatcgcc cgcgcccggg gcgaactcgt
     2041 agaaattgtt gtagctggtg acgtggtcgt acaaactcgg cgtctcggtc gtgctgagcg
     2101 gactcttgcg gatgttgggc agctttgcca ggccgccctg cgccgcggcg tcgggcacgg
     2161 gcaggcccgc cgccaccagc gcggcagccg acgcctggat gaactcccgg cggccaaggt
     2221 acacgttctt tggtgtgatc tcggacgaac ggatgccggg cgcctttttg atcagcatag
     2281 agcgattata ggaccgttcc cgtcgggttt cgcaaaccca tcggccgccg ttgctgtaca
     2341 tcctacggac gaatgagcct actggtttct gtgatcattc ccgtgctcgc ggacggccgg
     2401 gcagccgggc agctcgtcac acagcttcgc gtcgatccgc gggtcgaagt catcgtttcg
     2461 agcccagatt ccgggctcga gtcgctcgaa cacggcaggc ctgacctgag ggtcgtgcag
     2521 accgcgacag ggcgggcggt tcagatgaac tccggcgccg ggctcgcgcg cggcgattgg
     2581 ctgctctttc ttcacgccga ctcgcgagtg ccggacggct ggctggacgc gttggagaag
     2641 gctgccgcag cgggaaccgt cggcggatgg tttcgcttcg cgctggacga tccgtcatgg
     2701 caggcccggc tgatcgagcg gggcgtggcc ctgcgcatca agctgttccg gctcccctac
     2761 ggcgaccagg gcctcttcgt ccgtcggcag acgttcgcgg ccatcggcgg ctaccgcgat
     2821 atgccgctga tggaggatgt ggatttcgtg agacggctga cgcgggcggg cgaggtcgcg
     2881 gaaatcccgc tgccgatcgc gacatcggca cgccgctgga agcgtgacgg gtggtttcgg
     2941 cgaagcgcac gaaacctcac gcttctcgg
//
LOCUS       DBNGSH010000867         2182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4691681, whole genome shotgun sequence.
ACCESSION   DBNGSH010000867 DBNGSH010000000
VERSION     DBNGSH010000867.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4691681"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC16CY_19900"
     CDS             <1..219
                     /locus_tag="AC16CY_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3356125.1"
                     /translation="VFDPAIRTAEVTSAPALAVYAGTAQVPEVGAVKELFPNYEATQI
                     AGTGHFLMMEKPDEFNRVLTAFLDKIKF"
     gene            227..1147
                     /locus_tag="AC16CY_19905"
     CDS             227..1147
                     /locus_tag="AC16CY_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HIR3356126.1"
                     /translation="MRGQWPDLPYDAWKDTFATLHLWLQIAGKIRLVKTPWLNHSWHA
                     TFYVTSRGLTTSPIPQGERTFELEFDFIAHQLDIRSSDGRRGSVPLRPQSVAAFYGRV
                     IEALNALDIDVRINPKPSEMVDAIPLDRDEVHKSYDSEYAQRFWRVLVQADRVLKIFR
                     SRFIGKCSPVHFFWGGGDLAVTRFSGRPAPEHPGGIPNLADWVTREAYSHEVSSCGFW
                     PGGGPLPYPLFYSYGYPEPPGFAAAQVQPDGAFYSTDFREFVLPYDRVAGATAPDETL
                     LEFLQSTYDAASRLSNWDRGLVRHGAQESG"
     gene            complement(1208..>2182)
                     /locus_tag="AC16CY_19910"
     CDS             complement(1208..>2182)
                     /locus_tag="AC16CY_19910"
                     /inference="COORDINATES: protein motif:HMM:NF040438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HIR3356127.1"
                     /translation="AGHVGLDILTTTHVPFAEFNAERYKTLQVAPGQAADILAQVGAL
                     MKSKRPGNPLAALPVRRMVLAGTSASAAALINYLPAHMVYRLPDMKPIYDGFLPTSTG
                     ATIRQIDVPIIQVPTMTEVMSGTATARQDGDEAGNQFRVYEFPGMAHIDSRDAAAYYP
                     NPCKLPISRFPLAAYMSVALNHLWLWVDKGAVPPRADRILVDRNADNDGSLMALDEHG
                     NARGGIRNAYVDVPAKKYGVRNEGAVPPIPNAHPFVAVRGEAAQNQLCGLAGYEAAFT
                     PDQLRKLYKNKGEYTRRVERSLDDATRKGWFLPVYRGVVLADAAAVEF"
BASE COUNT          347 a          716 c          723 g          396 t
ORIGIN      
        1 gtgttcgacc cggccatccg gaccgccgag gtgacgtcgg cgcctgcgct ggccgtctac
       61 gcgggcacgg cgcaggtgcc agaggtcggg gcggtgaagg aattgttccc gaactacgag
      121 gccacgcaga ttgcgggtac cggccacttc ctcatgatgg aaaagcccga cgagttcaat
      181 cgcgtgctga cggcgttcct ggacaagatc aagttctgag cggaccatgc gcggccagtg
      241 gccggatctc ccgtacgacg cgtggaagga cacctttgcc acgctccacc tgtggctgca
      301 gatcgccggg aagatccgtc tggtcaagac gccgtggctg aaccattcgt ggcacgcgac
      361 gttctacgtc acgtcgcgag ggctgacgac gtcgccgatt ccgcagggcg agcgcacgtt
      421 cgagctggag ttcgatttca ttgcccatca gctcgacatc cggtcgagcg acggccgacg
      481 ggggagcgtt ccgcttcggc cgcagtcggt ggcggccttc tatgggcggg tcatcgaagc
      541 gctgaacgcg ctcgatatcg acgtccgcat caacccaaag cccagtgaga tggttgacgc
      601 tattcccctc gatcgagacg aggtccacaa gtcctacgac tccgaatacg cgcagcggtt
      661 ctggcgcgtg ctcgtgcagg ccgatcgcgt gctgaagatc ttcaggtcgc ggttcatcgg
      721 caaatgcagc ccggtgcact tcttctgggg gggcggcgat ctcgcagtca cccgcttctc
      781 ggggcgcccg gcgccagagc atccaggcgg aatcccgaac ctggccgact gggtcacgcg
      841 cgaggcctat tcgcacgagg tcagcagttg cggtttctgg cccggcggcg gcccgctgcc
      901 gtacccgctt ttctactcct acggctaccc ggagccgccc ggctttgcgg ccgcgcaggt
      961 ccaaccggat ggcgcgttct acagcacgga cttccgcgag ttcgtcctgc cttacgaccg
     1021 cgtcgctggc gccacggcgc ccgacgagac tctcctcgag ttcctgcagt ccacctacga
     1081 cgcggcgtcg agattgagca actgggaccg cggactcgtg aggcacggcg cgcaagagtc
     1141 aggctgaagc cggacactac gatcgatgct cagacgtacg tagtgtccgg tttcagccgg
     1201 accgtgctca gaactccact gccgcggcgt ccgcgagaac cacgccgcgg tagaccggca
     1261 ggaaccagcc ctttcgcgtg gcgtcgtcga ggcttcgctc gacccggcgc gtgtactctc
     1321 ccttgttctt gtacagcttc ctcaactgat ccggcgtgaa ggccgcttcg tatcccgcca
     1381 gcccgcacag ttgattctgc gccgcctcgc cccggacggc aacgaaggga tgcgcgttcg
     1441 gaatcggagg cactgcgccc tcgttccgga cgccgtactt cttcgccggc acgtccacgt
     1501 aggcgttgcg gatgccgcct ctcgcgtttc cgtgctcgtc gagcgccatc agcgagccgt
     1561 cgttgtcggc gttgcggtcg acgaggatcc ggtcggcgcg aggaggcacg gcgcccttgt
     1621 ccacccacag ccacaggtgg ttgagcgcga ccgacatgta ggcggcgagc gggaaccggc
     1681 tgatcggcag cttgcacggg ttggggtagt aggctgccgc atcacgcgag tcgatgtgtg
     1741 ccatgccagg gaattcgtag acgcggaact gattgcccgc ctcgtcgccg tcctggcggg
     1801 cggtcgccgt tccgctcatc acctcggtca tcgtcggcac ctggattatg ggcacgtcga
     1861 tctgccggat cgtcgcgccg gtactggtgg gcaggaaacc gtcgtagatc ggtttcatgt
     1921 ccggcagcct gtacaccatg tgtgccggca ggtagttgat gagcgcggcc gcggatgcag
     1981 acgtgcccgc gagcaccatc ctgcgaaccg ggagcgccgc cagcgggttg ccgggccgct
     2041 tcgacttcat cagcgcgccg acctgcgcaa ggatgtcggc ggcctgtccc ggcgcgacct
     2101 ggagggtctt gtagcgctcg gcgttgaact ccgcaaacgg aacgtgggtc gtcgtcagga
     2161 tgtcgaggcc aacatgcccg gc
//
LOCUS       DBNGSH010000868         1568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3332611, whole genome shotgun sequence.
ACCESSION   DBNGSH010000868 DBNGSH010000000
VERSION     DBNGSH010000868.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3332611"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="AC16CY_19915"
     CDS             complement(<1..980)
                     /locus_tag="AC16CY_19915"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3356128.1"
                     /translation="MLARTSTVTKGSADVAPWDPALADDRSGRGRLLRIPVEHIGIGA
                     PRPGRAGEDIQRLLAEVAARLAATEADSSDEGVVSDLRQVAGALQVAFDELQLREGAG
                     EDDSQGCPNVRLFGTSRLIVSESEAVRGALAQVEQVAPTPATVLLIGETGAGKEVFAQ
                     AIHELSQRHQRQMIRVSCAAIPSALIESELFGRERGAYTGAITRQMGRFEAASQSTLF
                     LDEIGELSAEVQVKLLRVLQERVIERLGSTQSIKVDVRIIAATNRNLEKAVEDKSFRE
                     DLYYRLNVFPIVVPPLRERLDDIPGLVWEFVNEFSKSLGKSISSISKDSMR"
     gene            complement(1009..1308)
                     /locus_tag="AC16CY_19920"
     CDS             complement(1009..1308)
                     /locus_tag="AC16CY_19920"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3356129.1"
                     /translation="MNASVSVSTLCRIVGRSERGLRNAFYRVHGIGPKRWMLEERLHG
                     ARRALSEVRSRPMTVTGVATDHGFYELGRFAATYKQAFGEAPSQTLRGAGRKSAA"
BASE COUNT          257 a          526 c          508 g          277 t
ORIGIN      
        1 cgcatcgagt ccttcgagat cgacgagatc gacttgccga gcgacttcga gaactcgttg
       61 acgaactccc acacgagccc cgggatgtcg tcgaggcgct cgcggagcgg cggtacgacg
      121 atggggaaga cattgaggcg gtagtacagg tcctcgcgga acgacttgtc ctcgaccgcc
      181 ttttccaggt tgcggttggt cgccgcgatg atccggacgt cgaccttgat cgattgcgtg
      241 cttccgagcc gctcgatcac ccgctcctgg agtacgcgca ggagcttgac ctggacttcc
      301 gccgacagct cgccgatctc gtcgaggaac agcgtggact ggctggccgc ctcgaatcgg
      361 cccatctgcc gcgtgatcgc gcccgtgtac gcgccccgtt cccggccgaa gagctcgctc
      421 tcgatcagcg cgctcggaat cgcggcgcag ctcacgcgaa tcatctggcg ctgatgccgc
      481 tggctcagct cgtgaatcgc ctgcgcgaac acctctttcc ccgcgccggt ctcgccgatc
      541 agcagcacag tggcaggagt gggcgcgacc tgctcgacct gagcgagagc cccgcgaacc
      601 gcttcgcttt cagacacgat caggcgcgag gttccgaaca accggacgtt ggggcaaccc
      661 tgcgagtcgt cctcgcccgc gccctcgcgc agctggagct cgtcgaaagc gacctgcagc
      721 gccccggcga cctggcgaag gtcgcttacg acgccctcgt ccgacgaatc ggcctcggtg
      781 gccgccagtc gcgcagcgac ctcagcgagc aggcgctgaa tgtcctctcc ggcccttccg
      841 ggccgtggcg cgccgatgcc gatgtgctcg accggaatgc gcaggagccg gccgcgccca
      901 gatcgatcgt cggcgagtgc ggggtcccac ggcgcgacgt cggccgatcc cttcgtaacg
      961 gtgctggttc gtgcaagcat ggccgtgccc tttcgtgttc ggacctgttc acgcagccga
     1021 cttgcggccg gcgcctcgca gcgtttgaga aggagcctcc ccaaacgcct gtttgtaggt
     1081 ggctgcaaag cgccccagtt cgtagaaccc gtggtcggtc gcgacgccgg tgaccgtcat
     1141 cggtctggag cgcacctcgc tcaaggcgcg gcgggctcca tgcaatcgct cctcgagcat
     1201 ccatcgtttg gggccaatac cgtgtacgcg ataaaacgcg ttacgcaggc cgcgctcgct
     1261 gcgtccgacg atgcggcaga gcgtggacac ggacacagaa gcattcatgt tggcgcgcag
     1321 gtacgtttcg gctagcgcaa ccgtcttctg ccacccggga atcgcaccgc gatcgcgccg
     1381 acatccacgt tgggctggtt tcatggccga agcaggctac atccgtctca ggggggggga
     1441 aactggccga tctgtcaggg tggaccggta aaaactgaca ggggggaggc gccgtctata
     1501 ctagccggaa tgaccatcgg ggcacggctt gcctccgtcg tggtactcgc ctggatggtc
     1561 gcctatcc
//
LOCUS       DBNGSH010000869        10219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4351369, whole genome shotgun sequence.
ACCESSION   DBNGSH010000869 DBNGSH010000000
VERSION     DBNGSH010000869.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4351369"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2088
                     /locus_tag="AC16CY_19925"
     CDS             <1..2088
                     /locus_tag="AC16CY_19925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3356130.1"
                     /translation="SIRGLVPADIRVTANHMAKPLRWRRGHPDAESTPWTVKNLFELK
                     NARRVLVDGNLFEHNWPDGQDGFAILLTVRNQSGSAPWSVVEDVVFANNLVRHAGAGI
                     NILGRDDNQPSEQTRRIAIRGNLFVDIGGRWGSGRLFQLLDGTRDISISHNTAFQTGS
                     VVVGGDHAPHAGFIFESNIALHNQYGISGSGTGTGQPTLDRYFPGAVVRRNVLIGGDA
                     ASYPRDNFFPATLEKAGFLKGPDGSYRVARRDGRAGREGPDIGADMESLMIAMSRVSG
                     GHRALTGRSWQGPEAVQLAGLAGGSTRSRSGALAFWASLCLLAYVYAGYPLVAWLRSR
                     LRPRLRVQAPAEPTVSIIVAAHNEGERIEARIENLLALDYPPGRREILIGSDGSTDGT
                     ASRASRYEDAGVRVEAFAEWRGKPAVLNDLVRRSTAEIVLFADARQRFDRGALRTLVS
                     HFADPSVGAVSGELMLVDGTGTSIGRGTSFYWRYEKFIRLHEGRAASTVGATGAIYAI
                     RRALFQPIPHDTILDDVLIPLRIVRQGYSVQFEPAARAFDAVSTTARQEYARKLRTIA
                     GTFQLFARERWLCNPLKNPLWFETISHKGLRLTLPVLHATALVANLALTDGVVYLGLL
                     AGQVGFYAAALAGHVFRHARRRPMFLTVPYAMCLMIWATLAGFTRFLAHRQLVTWEQS
                     APSVIPASSSSAR"
     gene            complement(2077..4020)
                     /locus_tag="AC16CY_19930"
     CDS             complement(2077..4020)
                     /locus_tag="AC16CY_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356131.1"
                     /translation="MSLAQRTCLDVIGLSAFVVIVAWACTSGLQGQSSGAPGGFMDSA
                     TSVGLRPRLSAAEIQTFLPSRGVFRFPSPYSTQGVRLTNSSDCGGADCVLPVGYSYWS
                     NINNHTNSNTMLIFLGLERRFGGGGPTLFSYDKLTGATLNLGPLFASNSALSWSTGEG
                     WYFSATRPTTLYLNSGPRMLRYDVQLRTLATVFDVSAQLGADKYLWQMHSSNDDRVHS
                     ATVRLTSTYDMLGCAVHNEATGLTTFFAKKGDFDECQVDRSGRWLVIKENVDGANGED
                     NRVIDLQTGAEQLLTDPNGAAGHSDIGFGYMLAEDNYHMDPGAVRLWNLALDMRGGQP
                     ATVQGQGDLVYRLSSWVSGLGHITYGNAHATVPIDQQRACVSNASRQDLPRVNEIVCF
                     PLDGSLNALIVAPNLTDLNAAGGGPDDYSKLPKGNIDVTGEYFIWTANAGTSRLDAFL
                     VRIPGGSTAPAPAPTPSPAPAPPPPAPAPAPVPSLSTAVTWTSLVNVTASTNSLRKTS
                     GCDGCPDAGAVSAQRIKSGDGHVSFSTTDTQRLRFIGLSTGNAGTSPTEIRFALRLHR
                     GVAEVREAGVYKADVAFASGDVFRIAVVGGAVTYAKNGSVFFTSQKAPVYPLLVDTAL
                     LDLNGTLSSVMISGSAASQDLSR"
     gene            complement(4512..5219)
                     /locus_tag="AC16CY_19935"
     CDS             complement(4512..5219)
                     /locus_tag="AC16CY_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356132.1"
                     /translation="MPMSECVVALRVWRRLGLFHLALGILDRWVRLVIPLRVLVVVAH
                     RIDAVRPAAVKNPEGLTARMLTPQQARNLADEGSVWYSPAFASEALAMGDRCLGVFEG
                     ERLLSYCWYSRGPTPAFNDTVVTVDSRYMYSYKAFTADRERGRGLHAYGVAAAAAQLA
                     AEGEIRGIVAFIEASNLSSLLSAQKIGDEVVGFVMLYRTGGEVRSLMTRGCATVGFRV
                     HHATTSDRPGAALEPIA"
     gene            complement(5261..5410)
                     /locus_tag="AC16CY_19940"
     CDS             complement(5261..5410)
                     /locus_tag="AC16CY_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HIR3356133.1"
                     /translation="MLYTIAVVLLILWLLGLVSGYTIGAFIHVLLVIAVVLFLAGLVS
                     RRRLA"
     gene            complement(5589..6464)
                     /locus_tag="AC16CY_19945"
     CDS             complement(5589..6464)
                     /locus_tag="AC16CY_19945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3356134.1"
                     /translation="MTPRSARPWLASSTFSYTDWDLHLFGLWLGARTLFSSPVAGLKS
                     LALPVEYIRCVETRYVLQHLEVRPGQRVLDIGSPKLLSLFLAATLHAEVHATDLVDYF
                     SDTYSRYSRVALGADHRRFCVRQEDARHLSYSRDHFDGVFSISAIEHIPDDGDSVAIQ
                     EIARVLAPGGTACLTVPWSDHGYLEEFKPPGDADMYWAQGHGERVFYQRAYDRDSLER
                     RLLRPSGLSLVDLSFWGERALPVEDVILNRRVPRIARMLLYPAHIALARLFLRRLTET
                     ETSHKKVACVTLRKT"
     gene            complement(6508..6981)
                     /locus_tag="AC16CY_19950"
     CDS             complement(6508..6981)
                     /locus_tag="AC16CY_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356135.1"
                     /translation="MLHYHVPGEQHRQHAEAVHATFDPLYATLDKENGMSLGVERPPI
                     PQARPNLNQGQVELIQRDISSLIGVVLARLLDESAPLPEAAQRIEGAHRWDLYDGVGR
                     NWLAEAKQYVTGQRQYPPSPEHVIAALEVAENLRRACDERDSLIAFLLQGENHAA"
     gene            complement(7060..7710)
                     /locus_tag="AC16CY_19955"
     CDS             complement(7060..7710)
                     /locus_tag="AC16CY_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3356136.1"
                     /translation="MKEPEFSVQGFLDAVGVAKNVGHYGRGDNVFTQGDVSKHVMYIQ
                     SGGVKLSVLSKAGKEAVVAMLGPGDFFGEGCLASQPLRMGTATATMPSAILLIRKAQM
                     SSLLHQQHAMSDRFISHMLTRNIRIEEDLVDQLFNSSEKRLARTLLLLARYGREARPV
                     RIVPQISQETLAEMVGTTRSRVNFFLNKFKKLGFIKYEGEHPIRINNSLLSVLLHD"
     gene            8391..8618
                     /locus_tag="AC16CY_19960"
     CDS             8391..8618
                     /locus_tag="AC16CY_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356137.1"
                     /translation="MSPVTFLLGFLMILSGFVILARRLHDRSMHESIAWLKAPGGDTG
                     GAVSAFAGLVVPILASITAGIGVVAVGVLVR"
     gene            complement(8664..9059)
                     /locus_tag="AC16CY_19965"
     CDS             complement(8664..9059)
                     /locus_tag="AC16CY_19965"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3356138.1"
                     /translation="MLSRVALATVVIASSVMSAAAQTQSAAPPAAPTGNAQRGKVLYE
                     TTLRCYACHGFDGQTGSPRLVPMTRTEDVFLAYVRKPATTGMPNFVSTPEQQLRDVYA
                     YVRAIPQAAPAPDSLPILKGILDRRSKAN"
     gene            complement(9102..>10219)
                     /locus_tag="AC16CY_19970"
     CDS             complement(9102..>10219)
                     /locus_tag="AC16CY_19970"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3356139.1"
                     /translation="AAVREAARLLVTAQSPLIQIAKVGRTPKAWDLLVELAETLQAPV
                     DVMGYSSWQKFPSWHPLYGTGGRGYTPDVTLGLEVTDMSAQARTARANGRKTISISAE
                     HLFQGSNIHDYGRYADVDLAIAADGDATLPVLIEEIRRQITPERKSAIQARGSRVAAA
                     HKEIQLASIGDARHGWDASPVSVPRLIAELGDQIANDDWAIVSGHQFTGDWQRRLLNH
                     DKPYRYNGDCGGFGIGYDTPASVGAALAHKKAGRLSVAIVGDGDLNFSPGVIWTAAHH
                     KIPLLLVVHNNRAYHAEVMIIQRMCGVRGRGNANSHIGNVIADPNPDYAQIAKGYGMY
                     SEGPIENPKELAGAYRRALAKVRAGEPALVDVVSQPR"
BASE COUNT         1696 a         3281 c         3415 g         1827 t
ORIGIN      
        1 tcgatccggg gactggtgcc cgccgacatc cgggtgacgg ccaaccacat ggccaagccc
       61 ctgcgctggc gacgggggca tccggacgcg gagagcacac cgtggaccgt gaagaatctc
      121 ttcgagctca agaatgcgcg ccgcgtcctc gtcgacggca atctgttcga gcacaactgg
      181 ccagacggcc aggacgggtt cgcgattctg ctgacggttc ggaatcaatc ggggagcgcc
      241 ccctggtcgg tcgtggaaga cgtcgtgttc gccaacaatc tggtgcgtca cgccggcgcg
      301 ggcatcaaca ttctcggtcg cgacgacaac cagccgagcg agcagacgcg ccgcatcgcc
      361 atccggggca atctctttgt tgacatcggc ggtcgatggg gttcggggcg gctgttccag
      421 cttctcgatg gaacgcggga catctccatc agtcacaaca ccgcgttcca gaccggatcc
      481 gtcgtggttg gcggagacca cgctccacac gccggattca tcttcgagag caacatcgcc
      541 ctgcacaacc agtacggcat cagcgggtcc gggaccggga ccggccagcc gacgctcgat
      601 cggtatttcc caggcgccgt cgtgcggcgc aacgtgctca tcggcggtga tgccgccagc
      661 taccctcggg acaacttctt ccctgccacg ctcgagaagg cgggtttcct caagggaccg
      721 gacggctcct atcgcgtggc gcgccgcgac gggcgggccg gacgagaggg gcccgacatc
      781 ggcgctgaca tggagtccct catgatcgcc atgagccgcg tctcgggcgg acatcgagcc
      841 ctcacgggcc ggtcctggca gggaccggaa gccgtgcagt tggccggcct tgccggcggg
      901 tcaacgcgca gccggtccgg tgcgctcgcg ttctgggcgt cgttgtgcct gttggcgtac
      961 gtgtacgccg gctaccccct ggtggcatgg ctgcgatcga ggctgcggcc tcgactccgc
     1021 gtccaggcgc cagcggaacc gaccgtgtcg atcatcgtcg cggcgcacaa cgagggcgag
     1081 cgcatcgaag cgcggattga gaacctcctc gcgctggact atccaccggg ccgccgtgag
     1141 atcctcatcg gctctgacgg ctcgaccgac gggacggcat cccgggcgag ccgttacgag
     1201 gatgccggcg ttcgcgtcga ggcattcgct gagtggcggg gcaagccggc cgtgctgaac
     1261 gatctcgtac gccgctcgac agccgagatt gtcctcttcg ccgacgcccg tcagcgcttc
     1321 gatcggggcg cccttcggac cctggtgtca cacttcgccg atccgagcgt cggcgcggtc
     1381 agcggcgagt tgatgctggt cgacggcacc ggcacgtcga tcggacgagg gacgtcgttc
     1441 tactggcgat acgagaagtt catccggctg cacgaaggtc gagcggcatc gacggtgggg
     1501 gcgaccggcg ccatctacgc gattcggagg gcgctcttcc agccgattcc ccacgacacg
     1561 attctcgacg acgtgctgat tccgttgcgc atcgtccgcc aggggtactc cgtgcagttc
     1621 gagccggcgg cgcgtgcgtt cgatgccgtg tcgacgacgg ctcggcagga atacgccagg
     1681 aagctgcgca ccatcgccgg cacgtttcag ttgttcgcgc gcgagcgatg gttgtgcaac
     1741 ccgctgaaga acccgctgtg gttcgaaacc atctcgcaca aggggctgcg gctcacgctg
     1801 ccagtgctgc acgccacggc cctcgtggcc aacctcgcgt tgaccgacgg ggtcgtgtac
     1861 ctgggtctcc tggccggaca ggtgggcttc tacgccgccg cgcttgccgg ccacgtgttc
     1921 cggcatgcgc ggcggcggcc gatgttcctc accgtgccct atgccatgtg tctcatgatc
     1981 tgggcgactc tcgcaggatt cacgcgtttc ctcgcccacc gtcagctcgt gacctgggag
     2041 caaagcgcgc caagcgtgat ccccgcgtcg tcgtcttcag cgcgataagt cctgcgaagc
     2101 ggcactgccg gagatcatca ccgacgacag cgtcccgttg agatcgagaa gcgcggtgtc
     2161 caccagcagg ggatacacgg gtgccttctg actggtgaag aagaccgagc cgttcttcgc
     2221 gtaggtgacc gctcctccga cgacggcgat ccggaagacg tcgccagagg cgaacgccac
     2281 gtcggccttg taaacgcctg cctcgcgcac ttcggcgaca ccgcgatgca gccgcaaggc
     2341 gaacctgatt tccgtcggac tggtcccggc attgccggtg ctcaagccga tgaagcggag
     2401 cctctgcgtg tccgtggtcg agaaggacac atggccatca cccgacttga ttcgttgagc
     2461 agacacggcg ccggcgtcgg gacacccgtc gcatccactc gtcttgcgca ggctgttggt
     2521 gctcgccgtc acgttgacga ggctcgtcca cgtcaccgcc gtgctgaggc tgggtactgg
     2581 ggcgggcgct ggcgcggggg gcggcggtgc cggcgctggt gagggtgtgg gcgccggggc
     2641 tggtgcggtg cttccgcccg gtattcggac gagaaacgca tcgagccgac tggtgccggc
     2701 gttggcggtc cagatgaagt actcgccggt gacgtcgatg ttccccttgg gcagcttcga
     2761 gtagtcgtct gggcccccgc cggcggcgtt caaatcggtc aggttggggg cgacgatcag
     2821 cgcgttgagc gatccgtcaa gaggaaagca gacgatctcg tttacgcgcg gcaggtcctg
     2881 ccggcttgcg ttgctcacgc aggcacgttg ctggtcgatc ggcacggtcg cgtgtgcgtt
     2941 gccgtacgtg atgtggccga ggcccgacac ccaggaggac aggcgataca ccaggtcgcc
     3001 ctgtccctga acggtggctg gttgtccacc ccgcatgtcg agtgcaaggt tccacaggcg
     3061 cacggcgccc gggtccatat ggtagttgtc ctcggcaagc atgtagccga acccgatatc
     3121 agaatgcccc gccgctccgt tcggatcggt gaggagctgc tcggcgccgg tctgcagatc
     3181 gatgacacgg ttgtcttcgc cgttggcgcc gtcgacgttc tccttgatca cgagccagcg
     3241 gccgctgcga tcgacctggc attcgtcgaa gtcgcccttc tttgcgaaga acgtggtgag
     3301 ccccgtcgcc tcgttgtgca cggcacaccc gagcatgtcg tacgtgctgg tcaggcgtac
     3361 ggtcgcggag tggacgcggt cgtcgttgct cgagtgcatc tgccacaggt acttgtccgc
     3421 accgagctga gcgctgacat cgaacacggt cgcgagcgtc cgcagttgca cgtcatagcg
     3481 cagcatgcgg gggccgctgt tcagatacag cgtcgtcgga cgggtggcgc tgaagtacca
     3541 gccctctccg gtgctccagc tcaacgcgct gttcgacgcg aagagggggc cgagattgag
     3601 cgtggcaccg gtcagcttgt cgtagctgaa cagcgtcggg cccccgccgc cgaaccggcg
     3661 ttcgaggccg aggaagatca gcatcgtgtt gctgttggtg tggttgttga tattgctcca
     3721 ataggagtag ccgaccggca ggacgcaatc cgccccaccg cagtcgctcg agttggtgag
     3781 tcgaacccct tgcgtcgagt acggcgaggg gaatctgaag acgccgcgtg atggcaggaa
     3841 cgtctggatt tccgctgccg aaagacgagg gcgaagaccg acgctcgtcg ccgagtccat
     3901 gaatccgccg ggtgcgccag agctctggcc ctgcagaccg gaagtgcacg cccatgcgac
     3961 gatgacaacg aaggccgata gaccgatcac atccagacag gtacgttgtg ccaaggacat
     4021 ggtggctcct tcactcggat gcgcaggcac gagtgttcta atcgacacac cacgagcgtc
     4081 atggcgatat ccggagtcag gattccgagg aatcccttgc cgggtcagtt gagtcggtgt
     4141 gcgtcgaagc gtccttctcg cccaatctgc gcatcggcaa ggccgaggct gaccgacagg
     4201 gcacccgccc aggggaggga ggagattgcc tgtattgagc gtgaagaccc gcggcggaag
     4261 gcttgagcta gttcagcggg cggagaataa ccgacccaga tcgactggtc ccccttcgat
     4321 ctgtgtctct aaccgtccga tgaatcgttg cttgtgcgac cgcgctaacg agcgtgggca
     4381 gtgtagcgtg caattttgaa cggcgtcaag gtgcccagcg tggtagtcgc ctttccgcgg
     4441 tctcgcgagc cgttccgtgc aggatgggcg atcgtcggaa atcggggccg cgtggggggc
     4501 gggcgcaccc gttaggcgat gggttcgaga gcggcgcccg gccggtctga tgttgtcgcg
     4561 tgatgtactc ggaatccgac cgtggcgcaa ccgcgcgtca tcagggaacg gacctcgccg
     4621 cccgtccggt acagcatgac gaagcccaca acctcgtccc cgatcttctg cgccgagagc
     4681 agcgacgaca ggttgctggc ctcgatgaac gccacgatgc cgcgaatctc cccttcggcc
     4741 gccagctgcg ctgccgccgc cgcgacgcca taggcgtgca ggccgcggcc gcgttccctg
     4801 tcagcggtga acgccttgta cgaatacatg tagcgggaat cgaccgtcac cacggtgtcg
     4861 ttgaacgcgg gcgtcggccc tctcgagtac cagcagtaac tgaggaggcg ctcgccctcg
     4921 aagacgccaa ggcatcgatc gcccatcgcg agcgcctcgg acgcgaacgc tggtgaatac
     4981 cagacgctcc cttcgtcggc gagattccgg gcctgctggg gagtcagcat ccgcgcggtc
     5041 agtccctccg ggttcttcac ggcggctggc cgcactgcgt cgatgcgatg agccacgaca
     5101 accaggactc gaagcggaat caccagtcgc acccagcggt ccaggattcc cagagccagg
     5161 tggaacaggc cgagccgccg ccagacacgt aaggcgacga cgcactcgga catgggcatg
     5221 aaactccctt gtccctatcc ttgtcgattc tcgagaacgg tcacgcgagc cgtcggcggc
     5281 ttaccagtcc ggccaggaac agcacgaccg cgatgaccaa caggacgtgg atgaacgccc
     5341 cgatcgtgta gccgctcacg aggccaagca gccacagaat caggagaaca acggcgatgg
     5401 tatacagcac ggtgcacctc tcgcactctg cggtgtgcaa cacatgcgcc atcaccgcgc
     5461 cgtcgcgagc cgcacagaag ccggccctcg aatcgcccct atggagaaag agggaatttg
     5521 tctcgaggct gtcggtaaat ctgcccgatc gggccgactg agcaggaccg cgccgccgcc
     5581 ggggggcgtc acgtcttccg gagcgtgacg caggccacct tcttgtgcga cgtctccgtc
     5641 tcggtcaggc gacgcaggaa gaggcgggcg agcgcaatgt gagccgggta gagcagcatc
     5701 cgggcgattc ggggaacgcg tcgattgagg atcacgtcct cgacaggcag cgcgcgctcg
     5761 ccccagaagc tgaggtcgac cagtgacagc ccgctcggcc ggaggagccg ccgctccagc
     5821 gagtctcgat cgtaggcgcg ctggtaaaag acccgctcgc cgtgtccctg ggcccagtac
     5881 atgtcggcgt cacctggcgg cttgaactcc tcgagatacc cgtggtcgct ccacgggacg
     5941 gtcaggcaag ccgtgccacc cggggcgagc acgcgcgcga tctcctggat ggcgacggaa
     6001 tcgccgtcgt cggggatgtg ctcgattgcc gagatcgaga acacgccgtc gaagtggtcc
     6061 cgggaatacg agagatgcct ggcgtcctct tgacgcacgc agaaccgacg atgatcggcg
     6121 ccgagtgcga cgcgcgagta acggctgtat gtgtcgctga agtagtcgac gagatcggtg
     6181 gcgtggacct ccgcgtgaag cgtggcggca aggaaaagcg acaggagctt ggggctgccg
     6241 atgtcgagga cccgctgacc ggggcggacc tcgagatgct gcaggacgta ccgggtctcg
     6301 acacagcgaa tgtactcgac tggcaacgcc aggctcttga gacccgcgac gggagacgag
     6361 aacagcgtgc gggcgccgag ccagagcccg aacaggtgga gatcccagtc cgtgtaggag
     6421 aacgtcgagc tggccagcca cggtcgcgcc gacctgggag tcatggtact cgaactcatg
     6481 gaggtaggaa ggtcgggcgt ccctgactca cgcggcgtgg ttctcgcctt gcagcagaaa
     6541 cgcgatcagc gagtcccgct cgtcacacgc ccgacgaagg ttctctgcca cctccagggc
     6601 cgcgatcaca tgctcggggc tcggcgggta ctgccgctgc ccggtgacgt actgcttggc
     6661 ctcggcgagc cagttccgcc cgacgccgtc gtacaggtcc cagcgatggg cgccctcgat
     6721 ccgctgagcc gcttcaggca acggcgcact ctcgtccaac agtcgagcga gcaccactcc
     6781 aatcagcgaa ctgatgtcgc gctggatcag ctcgacctgg ccctgattga ggttcggccg
     6841 tgcctgcggg atcggcgggc gctctacacc aagtgacatg ccgttctcct tgtcgagcgt
     6901 cgcgtagagc gggtcgaacg tggcgtgcac ggcctccgca tgctggcgat gttgctctcc
     6961 cgggacgtga tagtggagca tgagcattcc tttctatccg cttgggggcc ggccggcgcc
     7021 gctgtcgcgt gcggtgcgct cgggcgtcca tggctcacct cagtcgtgca ggagcacgct
     7081 cagcagggag ttgttgatcc ggatgggatg ttcaccctcg tacttgatga agcccagctt
     7141 cttgaacttg ttgaggaaga aattcacgcg cgagcgagtg gtgccgacca tctccgcgag
     7201 cgtctcctgt gagatctgcg gcacgatgcg caccggtctg gcctccctcc cataacgcgc
     7261 cagcagcagc aacgtacgtg ccagacgctt ctcgctggag ttgaagagtt gatcgaccag
     7321 atcctcttcg atccggatgt tgcgcgtcag catgtgcgag atgaaccggt cggacatcgc
     7381 atgctgctga tggagcagac tgctcatctg tgctttgcga atgagcagga tcgcactcgg
     7441 catggtggcc gtggcggttc ccatgcggag gggttggctc gccagacatc cttcgccgaa
     7501 gaagtcgccg gggccgagca ttgctaccac ggcctccttg cctgccttgg acaggaccga
     7561 cagcttgacg ccgcctgact gaatgtacat gacgtgcttg gagacgtcgc cctgcgtgaa
     7621 gacgttgtcg ccgcgcccgt agtgcccgac gttctttgcg actcccacag catcgagaaa
     7681 cccttgcacg ctgaattcgg gctccttcac agcctgcgtc gcgacgccgg actgccttcg
     7741 aggttcgatg gcctcaatca cggcgggagg ccgcggcacg gatgcgcgag caagcgcacc
     7801 ggccggcgga cgtgatcggg ttcttcgctg tgtcggtcgg agcacggaga gagcagcctg
     7861 ctttccacca ctcatgcagt cctcgggcgt cttgctgtgc ttggggtccg aagctcttgt
     7921 atcggcccgg cccgcaagcg acggaaaccc atctggaggg tagccgttat gcggtcaaag
     7981 tgtctgtcct gttctgaaca tgccctttcg ggcgggtatc caccggagcg ggcacgatat
     8041 cggcgccggg gtgggcgggg gctgttcaat ttcgaacagc gtgccggacc tggctgcggt
     8101 tatcgtcccc gccgtggctc ggctccagca gctggcgccc cggacgtacg tgacatgccc
     8161 acggtgtcaa tccccagcgg ctttcgtcac ctacgtgcgt gtgcagacac agtgttcctt
     8221 ctgtcccgag tgtcaattcc tttgggacac ggtccggtca cagtcgtcag gaaccttcat
     8281 caccggcccc gccgcgagtc ggcggcaaga cccccagcac cacccccccc gaaatccacg
     8341 gtgaaccagc gtgaccccca cgccgtccaa gaggtgttgg agaattcgaa atgagtcccg
     8401 tcacgtttct tctagggttc ctgatgatcc tctcgggctt cgtcatcctg gcccggcggc
     8461 tgcacgatcg atcgatgcac gagtcgatcg cgtggttgaa agcgcccggc ggcgacaccg
     8521 gcggcgcggt gtcggcgttt gccggcctgg tcgttccgat tctggcgtca atcacggccg
     8581 ggattggggt tgtcgcggtc ggcgtgctcg tgcgctgaca ttgcagattc gcccgaagtg
     8641 gcgcgcgggc ttcagcccgc gcgtcaattc gccttcgatc ggcgatcgag aatccctttg
     8701 aggatcggaa gactgtccgg cgcgggcgcg gcctgaggga tcgcgcggac gtaggcgtag
     8761 acgtcccgca gctgctgctc cggggttgac acgaagttcg gcatccctgt cgtggccggc
     8821 ttccgcacgt acgcgagaaa gacgtcttcg gtgcgagtca tcggcaccag gcgcggcgat
     8881 ccggtttgcc cgtcgaagcc gtggcacgca taacagcgca gcgtcgtctc gtacagcacc
     8941 ttgccgcgtt gcgcgttgcc ggtcggcgcg gccggcggcg cagcagattg agtttgcgct
     9001 gcagcgctca tcacgctcga tgcgatgaca acagttgcga gagcgacgcg gctcagcatg
     9061 agaaggcagc ctccgtgtct cggtgcctcg gtgtgtcgtg atcagcgagg ctgcgagacg
     9121 acgtcaacga gcgcaggttc tccggcgcgt accttggcca gtgcgcgcct gtaggcgccg
     9181 gccaattcct tcgggttctc gataggccct tcgctgtaca tcccgtaccc cttcgcaatc
     9241 tgcgcgtagt cggggttcgg atcggcaatc acgttgccga tgtgggaatt ggcgttgccg
     9301 cgcccgcgca cgccgcacat ccgctggatg atcatcactt cggcgtggta cgcgcggttg
     9361 ttgtgcacga cgagcagcag cggaatcttg tggtgcgccg ccgtccagat cacgccgggg
     9421 ctgaagttca ggtcgccgtc gccgacgatg gccaccgaca ggcggccggc tttcttgtgc
     9481 gccagcgccg cgccgaccga cgccggcgtg tcgtagccga tgccgaagcc tccgcagtcg
     9541 ccgttgtagc gatacggctt gtcgtggttg aggagccggc gctgccagtc gcccgtgaac
     9601 tggtggcccg agacgatcgc ccagtcgtcg ttggcgatct gatccccgag ctccgcgatc
     9661 aggcgcggaa cgctcaccgg gctcgcgtcc catccgtgac gcgcgtcgcc gatgctggcg
     9721 agctgaatct ccttgtgcgc tgccgccacg cgcgagcctc gcgcctggat cgcgctcttg
     9781 cgctccgggg tgatctggcg ccggatctcc tcgatgagca cgggaagcgt ggcgtcgccg
     9841 tccgcggcga tcgccaggtc cacgtccgcg tagcgtccgt agtcgtggat gttgctcccc
     9901 tggaaaaggt gctcggccga aatgctgatc gtcttccggc cgttcgccct ggccgtgcgc
     9961 gcttgcgccg acatgtcggt cacctcgagc ccgagcgtca cgtccggcgt gtagccgcgc
    10021 ccgccggtgc cgtacagcgg atgccaggac ggaaacttct gccacgatga gtagcccatg
    10081 acgtccacgg gcgcctggag cgtctccgcc agctcgacga gcagatccca cgccttcggc
    10141 gtgcggccga ccttcgcgat ctgaatcagc gggctctgcg cggtcacgag cagccgggcg
    10201 gcttcgcgca ccgccgcgg
//
LOCUS       DBNGSH010000870         2080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1635044, whole genome shotgun sequence.
ACCESSION   DBNGSH010000870 DBNGSH010000000
VERSION     DBNGSH010000870.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1635044"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="AC16CY_19975"
     CDS             complement(<1..1007)
                     /locus_tag="AC16CY_19975"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3356140.1"
                     /translation="MTSRTRLWVLAVSTPIIAFALVGGYLGQARGKDETYQHLRVFDD
                     VVSHVVNNYVEEVDLQQAMRGAMRGLSDALDPDSAYLPADLVKAIEQNSSAGPADVGL
                     ELTRQYYLRVVSARDGSPAARAGIRSGDYIRAVNSRATRDMSAVEGMRLMQGAAGSKL
                     ALLVIRGNAAEPHEVTLVRERVTGAETTTRMADASTGYVRVVEFTKNSPARLTQAFDA
                     LAKTGATRYVVDLRGTARGDFDDGIAAARLFVQSGTLAVRQTKARQETIGATSGDGAI
                     ASPAILLVDQGTAGAAEVFAAALRGNTRAELVGERTLGRAARQQIVKLPDGSALLLSS
                     AR"
     gene            complement(1025..>2080)
                     /locus_tag="AC16CY_19980"
     CDS             complement(1025..>2080)
                     /locus_tag="AC16CY_19980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR3356141.1"
                     /translation="AQRIGERIRVLEREAQRLAGEAQTLVGDLRKREIDRSLKVEQLK
                     QAEAERAETERALQQTTDRLSELELRRINQLPDVKAQLVGIYTRGRTGYARLIFGASD
                     LRELARASRAVSAMAAITERRLAEHERTLEALRQEKATFAKTARELQEREAAARRAKS
                     ASDRAVAAAAELITQIDSRRDLTAQYVGELQVAYNRIQQQVTALSSGKPADPVSVPLR
                     PFQGALDWPAQGRIIGAFGQPSDRLGGSLVRAGIEIGTTEDAPVRAVHGGTVGFAGPF
                     TGFGTLVILDHGSNSYSLYGYLESSGLQQGQAVEAGAEVGRVGAGPAGPPALYFELRI
                     DGKSVNPVQWLKPPLKR"
BASE COUNT          289 a          726 c          704 g          361 t
ORIGIN      
        1 cgagccgacg agagcagcag cgcgctgcca tccggcagct tcacgatctg ctgccgtgcg
       61 gcgcggccga gcgtccgttc gcccaccagt tccgcgcggg tgttgccgcg aagcgccgcc
      121 gcgaagacct cggccgcacc cgcggtccct tggtcgacga gcaggatggc cggagacgca
      181 atggcgccgt cgcccgaggt cgcaccgatc gtttcctgcc gggccttggt ctgccggacc
      241 gccagcgtgc cgctctggac gaagagccgc gccgccgcga ttccgtcgtc gaaatctccc
      301 ctggctgttc cacgcaggtc gacgacgtat ctggtggcac ccgtcttggc gagtgcgtcg
      361 aacgcctggg tcaggcgtgc gggcgagttc ttcgtgaact ccacgacgcg gacgtatccc
      421 gtcgaggcat cggccatgcg agtcgtcgtc tccgcgcccg tcacgcgttc gcgaacgagc
      481 gtgacttcat gcggctccgc ggcgttgccg cgaatgacga gcaacgccag cttggatccc
      541 gccgcgccct gcatcagacg catgccctcg acggcggaca tgtcgcgcgt ggcgcggctg
      601 ttgaccgcac ggatgtagtc gcccgaccgg attccggcac gcgcggccgg cgagccgtcc
      661 cgcgccgaga cgacgcgcag gtagtactgc cgcgtcaatt cgagtccgac gtcggcgggg
      721 ccggcgctcg agttctgttc gatggccttg acgagatcgg ccggcaggta tgcgctgtcg
      781 ggatcgagcg cgtccgagag gccccgcatc gcgccgcgca tcgcctgctg caggtccacc
      841 tcttccacgt agttgttgac gacgtgcgag acgacgtcgt cgaagacgcg gaggtgctgg
      901 taggtctcgt cctttccacg cgcctggccc agatagccgc cgaccagggc gaacgcgatg
      961 atgggcgtcg aaacggccag aacccagagg cgagtgcgtg acgtcatgaa tggactcccc
     1021 agccttaccg tttgaggggc ggtttcagcc attgtacggg attcaccgat ttaccgtcga
     1081 tccgcagctc gaagtagagc gccggcgggc ctgccggccc cgcgccgact cttcccactt
     1141 ctgcccctgc ttcgaccgcc tggccctgtt gcaagccgga tgactcgagg tagccgtaca
     1201 gggaatagct gttgctgccg tggtcgagga tcacgagcgt gccgaagccg gtgaacggac
     1261 ccgcgaaccc gacggttccc ccgtgcaccg cgcgaaccgg cgcatcctcg gtggtcccga
     1321 tctcgatacc agctctgacg aggcttccgc cgagccggtc ggagggctgg ccgaacgcgc
     1381 cgatgatccg gccctgcgcc ggccagtcga gcgctccctg gaaggggcgc agcggcactg
     1441 acacaggatc ggcgggcttt cctgaggaga gcgctgtgac ctgctgctgg atgcggttgt
     1501 aggctacctg cagctcgccg acgtactggg ccgtcaggtc ccgccgcgaa tcgatctgcg
     1561 tgatgagctc ggcggcggcc gccacggccc tgtccgaggc cgacttcgcc cgtcgcgccg
     1621 cggcttcccg ttcctgaagc tcgcgagctg tcttggcgaa cgtggccttc tcctgccgaa
     1681 gcgcctccag cgtgcgctcg tgctcggcaa ggcggcgctc ggtgatggcg gccatcgcgc
     1741 tcacggcgcg cgaggcgcgc gcgagctcgc gaagatcgct ggcgccgaag atcagtcgtg
     1801 cgtatcccgt ccgcccgcgc gtgtagatgc ccacgagctg ggccttgacg tccgggagtt
     1861 ggttgattcg ccgcagctcc agctccgata gtcgatcggt cgtctgctgc aacgcgcgct
     1921 cggtttccgc tctctccgct tcggcttgct tcagctgttc caccttgagg ctccggtcga
     1981 tctcgcgctt gcgcagatcg cccacgagcg tctgtgcctc gcccgccagg cgctgtgcct
     2041 cgcgctcgag gacacggatg cgctcgccga tgcgctgggc
//
LOCUS       DBNGSH010000871         2127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1126373, whole genome shotgun sequence.
ACCESSION   DBNGSH010000871 DBNGSH010000000
VERSION     DBNGSH010000871.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1126373"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="AC16CY_19985"
     CDS             <1..855
                     /locus_tag="AC16CY_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356142.1"
                     /translation="TVVFSHYRAVIKVVLGLILLGHAVAAGVLFAGGGTLAEFGSLLR
                     RFFDMAMVALVGITALAVLADRWIARSRILENWDPRSLGRDGHVGRAVRDVGRVVERA
                     GNAGFLARRAFDETSPPRVGSIPQLVVFAVLGAWCLLGLKFPSLMFGGPAPLAWSPLV
                     HRLYPFLVVAQAAILLYHYVRLKGPRDSVFLRFMRVAWANAGWILIVLLSMSDRQWLV
                     WAGTGDEWARFGSLVSVAGRTWSVVDLANGAITAAIVVVAAASLAGSFGRLSRIVSRR
                     STAHRALA"
     gene            893..1858
                     /locus_tag="AC16CY_19990"
     CDS             893..1858
                     /locus_tag="AC16CY_19990"
                     /inference="COORDINATES: protein motif:HMM:NF046861.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAS signaling domain-containing protein"
                     /protein_id="HIR3356143.1"
                     /translation="MRRRLPQRERDDIVAELRDALSSQIDAESAERGRPLTEDEVAAI
                     LKRYGRPRTVAARYGARDSLIGPNLYPAYLFSLRILAWIFVPIAGLSVLLAAMTADNV
                     AARVIEKAFLAVVMALVNLAIVTMVFAWIDRRTRWEETDDWDPHSLPAPFAPPGPTPR
                     PEAVGGICLMTFYLLLWTGVLPVGAWIGAANRWLSGTPLSYGFAPVWDAVNSLIIVLM
                     LTSIARDVASLLQPHRVMLRASVGVALHAGALVALVQLLRADALFVVADPALPGAGHV
                     GTFNMLLRMVFFVAAVGVAASGAFALRRLLRMARGRHQGMMPATS"
     gene            1928..>2127
                     /locus_tag="AC16CY_19995"
     CDS             1928..>2127
                     /locus_tag="AC16CY_19995"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR3356144.1"
                     /translation="MKLSDIGPGLLLAATGIGVGDMVSSTIAGAEYGLTLVWALAAGV
                     ILKFAITEGAARWQLATGTTLI"
BASE COUNT          256 a          719 c          734 g          418 t
ORIGIN      
        1 acggtcgtct tctcgcatta ccgggctgtg atcaaggtgg tgctgggcct gatcctcctg
       61 ggtcatgccg tcgcggcggg cgtgctcttc gctggtggcg ggacgctggc cgagttcggc
      121 tcgctcctcc ggcggttttt cgacatggcg atggtggcac tggtcgggat taccgcgctg
      181 gccgttctgg ccgatcgatg gattgcgcga tcccgcatcc tcgagaactg ggacccgcga
      241 tcgctcggtc gcgacggaca cgtgggacga gccgtccgcg atgtcggccg tgtcgtcgag
      301 cgagccggca acgccggctt cctcgcgcgg cgcgccttcg acgagacgag tccgccgcgg
      361 gtgggatcca tcccgcagct cgtcgtcttc gccgtcctcg gcgcctggtg cctcctgggc
      421 ctgaagtttc cgtcgttgat gttcggaggc cccgcgccgc tcgcgtggtc ccctctggtt
      481 catcgtctct acccgttcct cgtggtcgcg caggcggcga tactgctgta ccactacgtg
      541 cggttgaagg gtccgcgcga ctcggtgttt ctccgattca tgcgcgtcgc atgggccaac
      601 gccgggtgga tcctgatcgt gctgctgtcg atgtccgatc ggcagtggct ggtgtgggcc
      661 ggcaccggcg acgagtgggc gcggttcggg tcgctggtca gcgtcgccgg ccggacctgg
      721 tcggtcgtcg atcttgccaa cggcgcgatt acagccgcga tcgtcgtcgt cgccgcggcc
      781 agcctcgccg gatccttcgg caggctctcg cgtatcgtct ctcggcgctc gaccgcgcat
      841 cgcgcgctcg cgtgaggatg tcatgacact tctcgaacgc tatcttgacg ctgtgcgaag
      901 gcgcctcccg caacgggagc gcgacgacat cgtggcggag ctgcgcgacg cgctttcgtc
      961 gcagatcgac gccgaaagcg ccgaacgggg ccggccgctc accgaggacg aggtcgcggc
     1021 gatcctcaaa cggtacgggc ggccgaggac ggttgcggcg cgctacggcg cgcgcgactc
     1081 cctcatcggg cccaacctgt atccggccta cctgttctcg ctgaggattc tggcgtggat
     1141 cttcgtgccg atcgcgggac tgtcggtcct gctggcggcg atgaccgccg acaacgtggc
     1201 ggcgcgggtg atcgagaagg cgtttctcgc cgtcgtgatg gcgctcgtca acctggcgat
     1261 cgtcacgatg gtgttcgcgt ggatcgacag gaggactcgc tgggaggaga cagacgactg
     1321 ggatccgcat tcgctgccgg cgccgttcgc gccgcccggg ccgacaccgc ggccggaagc
     1381 ggttggcggt atctgtctca tgacctttta tctgctgctg tggaccggcg tactgccggt
     1441 gggcgcctgg atcggggcag ccaatcgctg gttaagtggc acaccgctct cgtacgggtt
     1501 cgcgcccgtc tgggacgcgg tcaattccct gatcattgtc ctgatgctga cgagcatcgc
     1561 ccgtgacgtg gcctcactcc ttcagccgca cagggtgatg ctgcgggcgt cggtcggagt
     1621 ggcgctccac gcgggcgcgc tcgtcgcgct cgtgcagctc cttcgcgccg acgcgctgtt
     1681 tgtcgtcgcg gatcccgcgc tgccaggggc cgggcacgtc ggcacgttca acatgctgct
     1741 gcgcatggtc ttcttcgtag ctgctgtcgg cgtcgcggca agtggcgcgt ttgcgctgcg
     1801 acgcctgctg aggatggccc gcgggagaca tcagggcatg atgcccgcca cctcgtgacc
     1861 cctgccccct gccccctgcc ccctgccccc tgcccccttc ccccttcccc ctgctacagt
     1921 ccgcatcgtg aagctctcag acatcggccc cggcctgctg ctcgccgcaa ccggcatcgg
     1981 cgtcggcgac atggtgtcct cgacgattgc cggcgcggag tacgggctga cgctcgtctg
     2041 ggcgctcgcg gcgggcgtga ttctgaagtt cgccatcact gaaggcgccg cacggtggca
     2101 gctcgccacg gggacgacgc tcatcga
//
LOCUS       DBNGSH010000872         2847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2484533, whole genome shotgun sequence.
ACCESSION   DBNGSH010000872 DBNGSH010000000
VERSION     DBNGSH010000872.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2484533"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="AC16CY_20000"
     CDS             complement(<1..542)
                     /locus_tag="AC16CY_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356145.1"
                     /translation="MRGTWRAFVLAAALSAAAAAPALAQVETAASPAVSREVMEQLLL
                     KGETIATKGKSKGVTEARRVTLRHESLTHDAQIQDVDISMPIFQVDPKHTEVDFKDSY
                     RYNIAAYRLSLLLGLDNVPMSVERVINGKPAAVTWWLDDVMMEEGDRLKRKENKYGPS
                     ALRTVSQTQMMSVFDELIQNR"
     gene            741..1235
                     /locus_tag="AC16CY_20005"
     CDS             741..1235
                     /locus_tag="AC16CY_20005"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3356146.1"
                     /translation="MRRFGAFELDLDAEQLLKNGRTIRIQPQPFRLLRLLTDRPGTLV
                     TREEIQAALWPNDTFVDFEQGVNFAIKQVREALGDRAEASVYVQTVPKRGYRFLAPVT
                     SGSEAQQSGQGGTTDPGLQKALWANIAELRIEDNRRRKQQTILIVALACVTVVLAVML
                     FARL"
     gene            1444..2364
                     /locus_tag="AC16CY_20010"
     CDS             1444..2364
                     /locus_tag="AC16CY_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356147.1"
                     /translation="MTSRRSASIAVAFFILALFYPQPCAAQVTINPGGTANITTATGS
                     GVACRIGDEPRSCNLSRFSGTEVSPGTNTVHAVYDQQAPTIGEANYAAARLHADIELA
                     GPPNSVVDVQIATTFDFDGAVSGAGAYKTTASASLHVTDITGSTALPVASHTLFQQER
                     SGDQGPSPSIDVAGESQTLRNDASTFRVTLRRGRTYRLTFEAQVLGEALVVGAVSSEA
                     RATWIRSAVTVDEDEADLLSIHDTAIQTALAAHDDAVRTELAQHDADIKQELAEIRER
                     LDDTNALLNEIKQLLLTPQGRREGFPIKVK"
     gene            complement(2391..>2847)
                     /locus_tag="AC16CY_20015"
     CDS             complement(2391..>2847)
                     /locus_tag="AC16CY_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HIR3356148.1"
                     /translation="GYESREQLDGDVELKARVERIRLLAGPMMNLGDVKDQTIPKMIL
                     LAPPRAGGVVSTRSFIPHRAHATIGVFAALSAATACLLPGSPAHSVAHIPEGRTKLML
                     VEHPTGASPVSITTGEQDGQVVVTEAAIISTARALFTGQVLVPDDGVRS"
BASE COUNT          490 a          936 c          922 g          499 t
ORIGIN      
        1 cggttctgga tcaactcatc gaacacgctc atcatctgag tctggctgac ggtacgcagg
       61 gcgctcggac cgtacttgtt ttcctttctc ttgagacggt ccccttcctc catcatcacg
      121 tcgtcgagcc accaggtcac ggcggccggc ttcccgttga tcacgcgctc gaccgacatt
      181 ggtacgttgt cgagcccgag gagcagcgag aggcgatagg ccgcgatgtt gtaccggtag
      241 gaatccttga agtccacctc ggtgtgcttc ggatcgactt ggaagatcgg catcgagatg
      301 tccacgtcct ggatctgcgc atcgtgagtg agcgactcgt gcctcagcgt cacgcgcctg
      361 gcctcggtca ctcccttgct ctttcccttc gtggcaatgg tctccccctt gagcaggagc
      421 tgctccatca cctcgcgcga cacagcgggc gacgcggcgg tctcgacttg cgcaagggcc
      481 ggcgccgcag ccgcggccga gagtgccgcc gccaggacga atgcacgcca ggttcctctc
      541 atcgcgacct ctcctctccg cgagtcctct ccgcgagttg ctccggctgc ggcacttata
      601 cgaggcgtcg cacgcccccg tccatgcgcg cgggcggagc cggcgacgat ctcccggcga
      661 gcggtcaacc ctgtctggat caaggggttg ggccggcgcc tatggagccg ggcgggacga
      721 agtggtaggc tgagcgggcc atgcggcgtt ttggcgcgtt cgagctcgac ctcgacgccg
      781 aacagctcct caagaacggc aggaccatcc ggatccagcc gcagccgttc cggctgctcc
      841 gcctgctcac ggaccggccc gggacgctcg tcacgaggga agagatccag gcggcgctct
      901 ggccgaacga cacgttcgtg gacttcgagc agggcgtgaa cttcgcgatc aagcaggtac
      961 gtgaagcgct cggcgatcga gcagaggcgt cggtctacgt tcaaaccgtg cccaaacgcg
     1021 gctaccgctt cctggcgccc gtcacctccg gctccgaggc tcaacagagc gggcagggcg
     1081 gaacgacgga ccccgggctc cagaaggcgc tctgggcgaa catcgccgaa ctgcgtatcg
     1141 aggacaaccg ccggcgcaag cagcagacga tcctgatcgt ggcgctggcg tgcgtgaccg
     1201 tcgtgctggc agtgatgttg ttcgcacggc tttgaccgca cccggcgcga ttgaagcgaa
     1261 gagccgcctg gacgcgcggc aatagtcgcc gcgtctgacg atttctctct acctccaaca
     1321 gccgcacgtc cgtcgtctgc cggtgcaacg agtggtaccg ttattgctcc gtttcgcgtt
     1381 ccgccccgtt ccgagccctt ggtcaatctc tgggagttcc tcgagtttgc gaaaggagaa
     1441 gccgtgactt cacgacggtc agcatccatc gcggtggcgt tcttcatact ggcgctcttc
     1501 tacccacagc cttgcgccgc gcaggtgacc atcaacccgg gcggcacggc gaacatcacg
     1561 accgcgacgg gtagcggcgt cgcatgtcgc attggtgacg agccgcgttc ctgcaacttg
     1621 agccgcttct cgggcacaga agtctctccg ggcacgaaca cggttcacgc cgtgtacgac
     1681 cagcaggcgc cgacgattgg cgaggccaat tacgcggcgg cgcgtctcca cgccgatatc
     1741 gagttggccg gtccaccgaa ctcggttgtc gacgtgcaga tcgcgacgac gtttgatttc
     1801 gacggagcgg tgtctggcgc gggtgcgtac aagaccacgg cgagtgccag tcttcacgtg
     1861 actgacatca ccggcagcac cgccctgccg gttgcgtcgc acacgctatt ccagcaggaa
     1921 cgatcaggcg accaggggcc gagcccgagc atcgacgttg ccggcgaatc gcagactctt
     1981 cgcaacgacg cctcgacctt ccgcgtcacc cttcgtcgcg gcaggacgta cagactgacc
     2041 ttcgaggccc aggtgctcgg cgaggcgctg gtcgtagggg cggtgagcag cgaagccagg
     2101 gccacttgga tcaggtctgc cgtcacggtg gacgaagatg aagcggatct cttgtcgatc
     2161 cacgatacgg caatccagac ggcactggca gcgcacgatg atgccgttcg cacggagctc
     2221 gcgcagcacg acgcggacat caagcaggaa ctggccgaga tccgcgagcg gctggacgac
     2281 accaacgcgc tgctgaacga gatcaagcaa ctgttgctga cgccacaggg tcggcgcgag
     2341 ggctttccga ttaaggtcaa gtgacgagcg ggtgaacgct tcagcaggaa tcaggatcta
     2401 accccgtcgt cgggcacgag gacctgcccc gtgaagagcg cccgggcggt actgatgatg
     2461 gcggcctcgg tgacgaccac ctgtccgtcc tgctccccgg tcgtgatcga caccgggctc
     2521 gcgccggtcg ggtgttcgac gagcatcagc ttcgtcctgc cttccggaat gtgcgccacc
     2581 gagtgcgccg gactgcccgg gagcaggcag gccgtggcgg cactgagcgc cgcgaagacg
     2641 ccaatcgtcg cgtgcgcgcg gtgcgggatg aacgatcgcg tcgagacgac gccgcccgcg
     2701 cgcggggggg cgagcaggat catcttcgga atcgtctgat ccttcacgtc gccgaggttc
     2761 atcatgggtc ccgcgagcag gcgaatgcgc tcgaccctgg ctttgagctc gacgtcgcca
     2821 tcgagctgct cgcggctctc gtagccc
//
LOCUS       DBNGSH010000873        13291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4352607, whole genome shotgun sequence.
ACCESSION   DBNGSH010000873 DBNGSH010000000
VERSION     DBNGSH010000873.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 13291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13291
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4352607"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /gene="rimO"
                     /locus_tag="AC16CY_20020"
     CDS             <1..1335
                     /gene="rimO"
                     /locus_tag="AC16CY_20020"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR3356149.1"
                     /translation="KNLVDSEVMLGLAQADGHQLTADAADADVLIVNTCAFIDKAKQE
                     SIDTILEMAEHKKTGSCRRLVVTGCMAERYRDELREQIPEIDAVLGTGEVPGIVGALG
                     TSAIPLLRANGEPIRPTGDRRPLPTYIYSADTPRLLATPRHYAYVKVAEGCDYACSFC
                     IIPTLRGRYRSRSPESIVEEASRLASRGVKELLLISQDTTFYGIDRHERGGLARLLRA
                     LDRVDGLEWVRMLYLYPTTITDDVLDAMAESGKVCKYIDLPLQHASDPVLKRMRRPGT
                     RAGYERLLDRIRRRVPGVTLRTTFIVGFPGETPDAYEELQSFVRSVEFDHVGVFTYSH
                     EEGTAAHALPDDVPAAVKRRRQAGLMRLQRRIVERAQRARVGQRVRVLVDGPSTEHDL
                     VLRGRLEGQAPDIDPLVYLTDCDPSSIASGQFVQAEIVGSRAYDLVARPASA"
     gene            1475..1951
                     /locus_tag="AC16CY_20025"
     CDS             1475..1951
                     /locus_tag="AC16CY_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR3356150.1"
                     /translation="MQPLEQIRAIAERVAASRGLEIWDIQSRRETGGHVVRVFIDRPG
                     PAARADESVSIEDCEQINREIGTILDVEDPLPFAYTLEVSSPGLDRPLRSLDDYRRFA
                     GRLAKVVVSEAVDNQKAFEGRLGGVVGDDVLLEAPNGRVHRLPARLITRGRLEVEF"
     gene            1959..3188
                     /gene="nusA"
                     /locus_tag="AC16CY_20030"
     CDS             1959..3188
                     /gene="nusA"
                     /locus_tag="AC16CY_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR3356151.1"
                     /translation="MQNPLMQTIEALAKEKGIEADTIITAIEDAVLTASRKYYKTNEN
                     LKTRFNPETGQVDLFAIKHIVSEVTNDATEISLKEAQDLYGNEAEVDMEIEFPKRTDV
                     LGRIAAQTAKQVIFQKVREAERENIFAEYNDRVGEVINSTVKRFENGDLIVEIGRVEA
                     VLPRKEQSRAESYTPGERIRAVIKGVSRSAKGPQIILSRTDPALLIKLFEQEVPEIYD
                     GTVMIRGAVREAGDRAKVAVYSRERDVDPVGACVGMKGTRVQAIIRELRGEKIDIVEW
                     SEDPVQLVTNGLSPAKVQRVSIVDDRERIMEVVVEDKQLSLAIGKKGQNVRLAAKLTG
                     WRIDIKSEEEKRREVEAEFGALGGGEGETAVAEEGAAETPEAAAAAEETADAGDVATV
                     TEDAKTDQTEDAKTDQT"
     gene            3224..6025
                     /gene="infB"
                     /locus_tag="AC16CY_20035"
     CDS             3224..6025
                     /gene="infB"
                     /locus_tag="AC16CY_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3356152.1"
                     /translation="MATVRIYKVAELLGTTSQEVLALLRRDHGIELKSASSTIEEVVA
                     RSFVERLARQRNVSLPGGDMFAEHAPPAKAASKKAAAPKKAEPPKPAAPVLGPPRLVK
                     TIRPPAPPAEAAAAAPAPIPAGPEPDVVDTPYPAAATVEVEPPGPSMAAAADAAQAVA
                     DVAPVEAPPGEEPIEEPAPTPPAAAAPAAAAQHPGRFVPPTIRLRIEEPGRTPQPARP
                     LTPAKRPAVSQPRIVQPPSPSQPAPSAKTTPSRAGASGQPARPPGYAPPRPTTPGMLG
                     GPRPLPSQPVRAQQPAFARPGAAPLTPARPPLGQRPPAGPARPGMRPTQTRRERPVQP
                     VIPSAPAAPPPITRTITLAEGMTVKDLADKLDVRVKDVLAKLLMRRMMMTINSTIETE
                     TAKDIAREFGAEIEMRSFEEELVSAQSDESKPEDIRPRAPVVTVMGHVDHGKTSLLDA
                     IRETKVAEREAGGITQHIGAYHVDVGDGRSVVFLDTPGHEAFTLMRARGAKVTDVVVL
                     VVAADDGVMPQTREAIDHAKAANVPIVVAINKIDKHGANPDNVKRELAELGLVPEDWG
                     GQTVTVPVSAKKRQNLDQLLEMILLSTDILELRANPKRNASGTVLEAKLDRGRGPVAT
                     VLVQDGTLSVGDNFIAGPVVGKVRALIDDRGRPVKSAAPATPVEVLGLTSLPQPGDPF
                     QTVADAAKARQIATFRQAQAKERALGAKGGRLTLESLKEQIAEGGMKELPLIVKTDVQ
                     GSAEVLADTLTKLSDEKVKIRIIRSGVGAINESDVLLASASNAIIIGFNVRPDRNAHD
                     MAEQEKIDIRLHSVIYNVTEEIKKSMAGLLEPTFKEVRIGTAEVREVFRVPKVGMIAG
                     CLVTDGRITRAGETQARLLRDNVVVFEGKIASLRRFKDDVSEVKSGLECGIGLQNYND
                     LKVRDMIEVFTMERVAVPV"
     gene            6037..6318
                     /locus_tag="AC16CY_20040"
     CDS             6037..6318
                     /locus_tag="AC16CY_20040"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR3356153.1"
                     /translation="MRVAVLRVELHIPHSQSLKDKRMVLRRVKDRLQKLNVAVAEVEH
                     QDAWQRAELGIVAVSTTAVHVEQQLMAAADEIERIEPGLVTRTEIDFLT"
     gene            6321..6689
                     /gene="rbfA"
                     /locus_tag="AC16CY_20045"
     CDS             6321..6689
                     /gene="rbfA"
                     /locus_tag="AC16CY_20045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3356154.1"
                     /translation="MAQGHRPDRVGERIREELSEMLSRGVVHDPGIGFITLTRAKVSP
                     DLQVARVYYTTMGDANAKRETAAALRRATPFFRRQMGERLGLRRVPELEFRFDESVEQ
                     QDRIEQILRELHEEDERRSR"
     gene            6686..7696
                     /locus_tag="AC16CY_20050"
     CDS             6686..7696
                     /locus_tag="AC16CY_20050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR3356155.1"
                     /translation="MTTTSADTTAAQVAAEIRRRQRFVVVSHARPDGDAVGSCVAMAL
                     SLEQLGKQVRVVSRDRVPPQMEAFAGVGAIEVVDRIDDPGDAVIVMECGDVQRTGVAG
                     LDRGFVINIDHHPGNTTYGAINWFDLSAAACGEMVFELISELGVTLTRDIATHVYVAI
                     LTDTGSFHYSNITPRTFDICRQCIDAGVNPSLVARTIFDSNNLGRLKLFGAVLHQMQL
                     DASGRIATLWVDQKLARDCGGTYEDTEGLVNLPLTVRDIQAVVFFKENGPGDWRVSLR
                     SKGEIDVNAIAREFGGGGHVNASGCSASGALADLRPVFEKKLTEAVQQWMASSSSTSR
                     RP"
     gene            7657..8541
                     /gene="truB"
                     /locus_tag="AC16CY_20055"
     CDS             7657..8541
                     /gene="truB"
                     /locus_tag="AC16CY_20055"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3356156.1"
                     /translation="MDGVLVVDKPQAVTSHDVVAEARRRLGQPRLGHTGTLDPLATGV
                     LPLACGRATRLVRFLTAADKEYEAGLRFGVSTDSYDIMGRELMRSGLTPSKEAVERAL
                     ASLRGEYLQVPPAHSAKRVGGRRAYELARRDEEVTLAPVRVRVTRADLVTLDGASAIV
                     AITCSSGFYVRSFAHALGELTGTGACVESLRRTRHGEFSLSQAVTVEQLRQGAAAVGD
                     RWIPLDRLLPGLPSVRVSAEGRTRVSHGQELQGSHLLDEAVEGSDSWIRLLDEDNHLL
                     AVATASSARALHPAVVLI"
     gene            8767..9036
                     /gene="rpsO"
                     /locus_tag="AC16CY_20060"
     CDS             8767..9036
                     /gene="rpsO"
                     /locus_tag="AC16CY_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3356157.1"
                     /translation="MALSRDRKSGIIGTYRTHDGDTGSPEVQVALLSERINYLTEHFK
                     IHAKDHHSRRGLLKLVGQRRRLLDYLKSKDSGRYAEIIERLGIRK"
     gene            9048..11183
                     /gene="pnp"
                     /locus_tag="AC16CY_20065"
     CDS             9048..11183
                     /gene="pnp"
                     /locus_tag="AC16CY_20065"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3356158.1"
                     /translation="MHNVHLQTREIRIGSRTVSLETGKLAKQADGSVLVRSGDTVVLV
                     TAAHAASAREGIDFLPLTVDYREYTYASGRIPGGFFKREGKPSEKEVLTSRLIDRPIR
                     PLFTSGWRYETQIIALLMSADTDNDADVLAITGASAALALSEIPFEKTIAGVRVGIVD
                     GAFVINPTFTERKASTLDLVVAGTRDGIVMVEAGAREVSEAQVVGALEAAHAAIKTIV
                     ASIDDLKAAAGKKKLTVQTKAIGHEFYREVEEKVYVPLGEAMRLNGKLESYQRVDQVL
                     EDLIASIPEGELERKVEAKKIFSELKEKVLRDSVLDKSVRLDGRKFDEIRPIWCETGV
                     LPRTHGSVVFTRGETQALVTCTLGTADDEQKIEHVAGEFYKRFMLHYNFPPFSVGEVQ
                     FLRGPGRREVGHGALAERALAPMVPSEDAFAYTIRLVSDILESNGSSSMASVCGGSMA
                     MMDAGVPLKNPVAGIAMGLIMDEQSGRWAVLSDIAGAEDHYGDMDFKVAGTAHGITAL
                     QMDIKVTGITSEIMAKALEQARAGRMHILGEMAKTIAAPRRDMSMFAPRIVTIKIPVD
                     KIRDVIGPGGKMIRSIIERTGVKIDVEDDGRVNVASADGESAAKAIGIIQELTATPEL
                     NKTYMGKVQRITDFGAFVEIMAGTDGLLHVSEIANHRVKDVRDELKEGQQILVKVINI
                     DPTGKIRLSRKALLQEEAQKAASAPAAKE"
     gene            11272..11658
                     /locus_tag="AC16CY_20070"
     CDS             11272..11658
                     /locus_tag="AC16CY_20070"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3356159.1"
                     /translation="MVLKKRAVRLLLAGMAGLAIGPAPFGVAGQDQAPNRREIALTAK
                     NYRFSPSRIEAAQDDLLTLTVQSEDVAYGFTIDEYRVSRRVPAGGMAMLQFRADRPGK
                     FIFYSNLTNDARHAQMKGELIVRARQ"
     gene            complement(11660..12772)
                     /locus_tag="AC16CY_20075"
     CDS             complement(11660..12772)
                     /locus_tag="AC16CY_20075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3356160.1"
                     /translation="MANEDKATRYQRLRRRTFLLGTAWQAVLLVAILVTGGSSALRLA
                     IESRVGPGFAIVCFLYVLAIGVLYELVRLPLAFYHGVVLERRYSLSTETTGRWWTDHV
                     TSSAITLGCLVAGALLVSSLLRWSPDRWWIAAAVSFAATLVLLAKLMPAVLLPFFYDV
                     KPLAREPLGSRLLALADRAGARVLGVFEWRVGDRTRKASAALVGIGSGRRILVSDTLL
                     AEHSDEEIEAVLAHEIAHDARGHVWSAIALEAGLIALGCYAADAALTVLGPALDLEGK
                     SDIAALPLVALAVGAVSFASMPVTNALSRAHERTADRDALEMTRNQAAFISAMKRLAG
                     RNLAEERPSRLIELLFHTHPSTAARIEAAKQLDSRL"
     gene            complement(12783..>13291)
                     /locus_tag="AC16CY_20080"
     CDS             complement(12783..>13291)
                     /locus_tag="AC16CY_20080"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3356161.1"
                     /translation="AEGEGGRGQARSRVTIRYEVRIPPGLDVLLRTQNGDVRMEGLQG
                     TVTASATNGTLTGRQLSGRVDASTVNGGVDVSLESIAGDSRFSAVNGGVRMNVGPGVN
                     AELDATVVNGGVRISDDVTFAADQRTPQRVDGRIGSGGPRIVVHVTNGGITIGSREGR
                     VTTRRDRQ"
BASE COUNT         2402 a         4428 c         4552 g         1909 t
ORIGIN      
        1 aagaacctcg tcgactccga ggtgatgctg gggctcgcgc aggcggacgg ccatcagctc
       61 accgctgacg ccgctgacgc cgacgtcctc atcgtcaaca cctgcgcgtt catcgacaaa
      121 gcgaagcagg aatcgatcga cacgatcctc gagatggccg agcacaagaa gaccggctcg
      181 tgccggcggc tcgtcgtcac cgggtgcatg gccgaacgct accgcgacga gctgcgcgag
      241 cagattccgg agatcgatgc ggtgctgggc acgggcgagg tgcccgggat cgtgggcgcg
      301 ctcgggacgt cggcgattcc tttgctgcgc gcgaacggcg agccgatacg accgaccgga
      361 gaccggagac cgcttccgac ctacatctac tccgccgaca ctccgcggct gctggcaacc
      421 ccccgtcact acgcctacgt gaaagtcgcc gaagggtgtg actacgcctg ctccttctgc
      481 atcatcccca ccttgcgagg ccgataccgg agccgctcgc cggagtcgat tgtggaggag
      541 gcgtcacgcc tggcctcgag gggcgtcaag gagctcctcc tgatctctca ggacacgacc
      601 ttctacggca tcgatcgaca cgagcgcggc gggctcgccc gcctgctccg cgccctcgac
      661 cgcgtggacg gcctcgagtg ggtccggatg ctctacctct atccgacgac gatcaccgac
      721 gacgtcctcg acgccatggc cgagagcggc aaggtctgca agtacatcga tctgcctctg
      781 cagcacgcct cggaccccgt gctcaagcgc atgaggcgcc cgggaacgcg cgccggctac
      841 gagcgccttt tggaccggat ccggcgccgg gtccccggcg tcacgctgcg gacgacgttc
      901 atcgtcggct tccctggcga gacaccggac gcctacgaag agctccagtc gttcgtccga
      961 agcgtggagt tcgaccacgt gggcgtgttt acgtattcac acgaggaagg cacagccgcc
     1021 catgccctgc ccgatgacgt gccggcggcg gtcaagcgcc ggcgccaggc cggcctgatg
     1081 cggcttcagc gccggatcgt ggagcgggcg cagcgggcgc gtgtcggcca gcgcgtccgc
     1141 gtcctggtgg acggcccgtc aaccgagcac gacctcgtcc ttcgcgggcg gctcgaaggc
     1201 caggcgcccg atatcgatcc gctcgtctac ctgaccgact gcgacccgtc gtcgatcgca
     1261 tccggacagt tcgtccaggc cgagattgtg ggcagccgcg cctacgatct ggtcgcgcgt
     1321 cccgcctccg catagcttcg gcgaggctcg ccgaagcgcg tgagcgcgaa gtgcggccgt
     1381 tgtcgggggc tgatccagcg tgataagctt gagggtcacc tacggaggca aactctaagt
     1441 gggctcgtgc ccactttttt tgtttcaggt tgccatgcag ccgctggagc agattcgggc
     1501 gattgccgaa cgggtggcgg cctcgcgcgg actggagatt tgggacatcc agagccggcg
     1561 cgagacggga gggcacgtgg tcagggtctt catcgacagg ccgggaccgg cggcccgcgc
     1621 ggatgagagc gtgagcatcg aagactgcga acagatcaac cgggagatcg ggacgattct
     1681 cgacgtcgaa gacccgctgc ccttcgcgta cacgctggaa gtgtcctcgc cgggactcga
     1741 ccggccgctc cggtcgctcg acgactaccg ccgcttcgcc ggcaggctgg cgaaagtagt
     1801 ggtgagcgaa gcggtggaca accagaaggc gttcgagggg cgtctcggag gcgtcgtcgg
     1861 cgacgacgtg ctgctcgaag cgccgaacgg gcgggtgcac aggctgcccg cgcggctcat
     1921 cacgcgcggc cggctcgagg tcgagttctg aggaagacat gcaaaacccg ttgatgcaga
     1981 cgatcgaggc gctcgccaag gaaaaaggca tcgaagccga cacgatcatc accgcgatcg
     2041 aggacgccgt cctgaccgcg tcgcgcaagt actacaagac caacgagaac ctgaagacga
     2101 ggttcaaccc cgaaacggga caggtcgatc tgttcgcgat caagcacatc gtgtccgagg
     2161 tcaccaacga cgcgacggag atctcgctga aggaagccca ggatctctac gggaacgaag
     2221 cggaagtgga catggagatc gagttcccga agcgcacgga cgtgctcggg cgcatcgcgg
     2281 cccagaccgc gaaacaggtg atcttccaga aagtgcggga ggcggagcgc gagaacatct
     2341 tcgccgagta caacgaccgc gtcggcgagg tgatcaacag cacggtgaaa cgcttcgaga
     2401 acggcgatct gatcgtggag atcgggcgcg tcgaagcggt gctgccccgc aaggagcagt
     2461 cccgcgccga gagctacacg cccggcgagc ggatccgcgc cgtgatcaag ggggtcagcc
     2521 ggagcgccaa gggacctcaa atcatcctgt ctcgcacgga cccggcgctg ctcatcaagc
     2581 tgttcgagca ggaggtgccg gagatctacg acggcacggt gatgattcgc ggggcggtcc
     2641 gtgaagcggg cgaccgagcc aaggtggcgg tctactcgcg cgagcgtgac gtcgatccgg
     2701 tcggggcgtg cgtcgggatg aaaggcacgc gcgtccaggc catcatccgg gagctgcgag
     2761 gcgagaagat cgacatcgtc gaatggtccg aggatccggt gcaactggtc acgaacggct
     2821 tgagcccggc caaggtgcag cgcgtgtcaa tcgtggacga ccgcgagcgc atcatggaag
     2881 tcgtcgtcga ggacaagcag ttgtcgctcg cgatcgggaa gaagggacag aacgtgcgcc
     2941 tggcggccaa gctgaccggg tggcggatcg acatcaagag cgaggaggag aaacggcgcg
     3001 aggtggaggc cgagttcggc gcgctgggag gtggcgaggg cgagaccgcg gttgccgagg
     3061 aaggcgctgc ggagactccg gaggcggctg ctgcagctga ggagaccgcc gacgccggcg
     3121 acgtggcgac cgtgaccgaa gacgcgaaga cggatcagac cgaagacgcg aagacggatc
     3181 aaacgtaaag cgcgggcgtc gccctgcgcg gacggggagc aatttggcaa cggttcggat
     3241 ttacaaggtc gcggaactgc tcggcacgac gagccaggaa gtgttggcgc tgctcaggcg
     3301 cgatcacggg atcgagctga agagcgcgtc cagcacgatc gaggaggtcg tcgcgcgttc
     3361 gttcgtcgag cgcctcgcca ggcagcgcaa cgtctcgctt ccgggcggcg acatgttcgc
     3421 cgagcacgcc ccgccggcca aggcggccag caaaaaggct gctgccccga agaaggcgga
     3481 accgccgaag cccgccgcgc cggttctcgg accgccgcgc ctcgtcaaga ccatcaggcc
     3541 gccggcgccg cccgcagaag cggcggccgc cgcaccggct cccatacccg cgggccccga
     3601 gcccgacgtg gttgacacgc catatcccgc tgccgcgaca gtcgaggtgg agccacccgg
     3661 cccgtcgatg gccgctgccg ccgatgccgc acaggcggtg gctgatgtcg ccccggtcga
     3721 agcgccacct ggcgaggagc cgatcgaaga gccggcgccg acgccgcccg ccgccgctgc
     3781 gccggcagcg gcggcacagc acccgggccg gttcgtgccg ccgacgatcc ggcttcgaat
     3841 cgaggagccc gggaggacgc cgcagcctgc gcggccgttg acgccggcca agcgtcccgc
     3901 ggtctcgcag ccgcgcatcg ttcagccgcc ttcgccgtca cagcctgctc cgtccgcgaa
     3961 gacgacaccc tcgcgcgcgg gcgcttcagg tcagcccgcg cgtccgcccg ggtatgcgcc
     4021 gccgcggcct acgacgcccg gcatgctcgg cggaccgagg cctctgccct cgcagcccgt
     4081 gcgcgcgcag cagcccgcgt tcgcccggcc gggcgccgcc ccgttgacgc cggccaggcc
     4141 gccgctcggt caacgtcccc ccgcgggccc ggctcgaccc gggatgcggc cgacgcagac
     4201 ccggcgcgaa cggccggtgc agccggtgat tcccagcgcg ccggcggcgc ctccgccgat
     4261 cacccgcacg atcacgctgg ccgaagggat gacggtcaag gacctggccg acaagctgga
     4321 cgtccgcgtg aaggacgtgc tggccaagct gttgatgcgc cgcatgatga tgacgatcaa
     4381 cagcacgatc gagacggaaa cggccaagga catcgcgcgc gagttcggcg ccgagatcga
     4441 gatgcggagc ttcgaagagg agctcgtctc ggcccagagc gacgagtcga agccggagga
     4501 catcaggccg cgcgcgcccg tcgtgacggt catgggccac gtcgatcacg gcaagacgtc
     4561 cctgctcgac gcgattcgcg agacgaaggt cgccgagcgc gaggccgggg gcatcacgca
     4621 gcacatcggc gcgtaccacg tcgacgtggg cgatgggcgc agcgtcgtgt tcctcgacac
     4681 gcccggccac gaagcgttca cgctgatgcg cgcgcgcggc gcaaaggtga ccgacgtggt
     4741 ggtgctcgtc gtggctgccg acgacggcgt gatgccgcag acgcgcgagg cgatcgatca
     4801 cgccaaggcc gccaacgtgc cgatcgtcgt ggcgatcaac aagatcgaca agcacggcgc
     4861 gaaccccgac aacgtgaagc gcgagctcgc cgagctcggt ctcgttcccg aggactgggg
     4921 aggacagacg gtcaccgtgc ccgtgtcggc caagaagcgg cagaatctcg accagctcct
     4981 cgagatgatc ctgctgtcca ccgacatcct cgagctgcgc gccaacccga agcggaacgc
     5041 ctcgggaacg gtgctcgaag cgaagctcga tcgcgggcgc gggcccgtcg cgacggtcct
     5101 ggtccaggac ggcacgctca gcgtgggcga caatttcatc gcggggcccg tcgtcggcaa
     5161 ggtccgcgcg ctcatcgacg accgcggccg tcccgtcaag tcggctgcgc cggccacgcc
     5221 cgtggaggtg cttggactga ccagcctgcc ccagcccggc gacccgttcc agacggtcgc
     5281 ggacgcggcg aaggcgcggc agatcgcgac gttccgccag gcgcaggcca aggagcgagc
     5341 gctcggggcg aagggcggaa ggctgacgct cgaatcgctg aaagaacaga tcgcggaagg
     5401 cggcatgaag gagttgccgc tcatcgtcaa gacggacgtg caggggtcgg ccgaggtgct
     5461 cgccgacacg ctcacgaagc tgtcggacga gaaggtgaag atccggatca tccggtccgg
     5521 ggtcggcgcc atcaacgagt cggacgtgct gctggcgtcg gcgtccaacg ctatcatcat
     5581 cggcttcaac gtccggcccg accgcaacgc gcacgacatg gccgagcagg agaagatcga
     5641 catccgcctg cactcggtga tttacaacgt gaccgaggag atcaagaaga gcatggccgg
     5701 cctcctcgag ccgacgttca aggaggtccg gatcgggacg gccgaagtgc gcgaagtctt
     5761 ccgggtcccg aaggtgggca tgatcgcggg ctgcctggtg accgacggcc gaatcacgcg
     5821 cgcgggcgag acgcaggcgc gcctacttcg cgacaacgtc gtggtcttcg aaggcaagat
     5881 cgcgtcgctg cgccgcttca aggacgacgt gagcgaagtc aagagcgggc tcgagtgcgg
     5941 aatcggcctg cagaactaca acgacctgaa ggtccgcgac atgatcgagg tgttcacgat
     6001 ggagcgcgtg gcagtcccgg tctagcgaac ctggacatgc gcgtcgccgt gctccgggtc
     6061 gagcttcaca ttccgcactc gcaatcgttg aaggacaagc gcatggtgct ccgacgggtc
     6121 aaggaccggc tccagaagct caacgtcgcc gtcgccgaag tcgagcacca ggacgcctgg
     6181 cagcgggccg agctcggcat cgtcgccgtc tcgacgacgg ccgtgcacgt cgagcagcag
     6241 ctcatggccg cggccgacga aatcgagcgg atcgaaccgg ggctcgtcac gcggaccgag
     6301 atcgacttcc tgacgtaacc atggctcaag gacaccgccc ggaccgcgtc ggcgaacgga
     6361 tccgcgaaga gctcagcgag atgctgagcc gcggggtcgt gcacgacccg gggatcgggt
     6421 tcatcacgct gacgcgcgcc aaggtctcgc cggacctcca ggtggcgcgc gtgtactaca
     6481 cgacgatggg cgacgccaac gcgaaacggg agaccgccgc cgccctccgc cgcgccacgc
     6541 cgttcttccg gcgccagatg ggcgagcgcc tcgggctgcg gcgcgtgccg gagctcgagt
     6601 tccggttcga cgaatccgtc gagcagcagg atcggatcga gcagatcctc cgcgagctgc
     6661 acgaggagga cgagaggcgc tcccgatgac cacgacgagc gccgacacga cggccgcgca
     6721 ggtcgccgcg gagattcgac gccggcagcg attcgtcgtt gtctcgcacg ccaggccgga
     6781 cggcgacgcc gtcgggtcgt gcgtcgcgat ggcgctctcg ctcgagcagc tcggcaagca
     6841 ggtccgcgtc gtgagccgcg accgcgtgcc gccgcagatg gaggcgtttg ccggcgtcgg
     6901 cgccatcgag gtcgtcgatc ggatcgacga ccccggcgat gccgtgatcg tgatggagtg
     6961 cggcgacgtg cagcgaaccg gcgtcgcggg cctcgatcgc ggcttcgtca tcaacatcga
     7021 tcaccacccg ggcaacacga cgtacggcgc gatcaactgg ttcgacctct cggccgccgc
     7081 atgcggcgag atggtcttcg agctgatctc cgagctcggc gtgacattga cacgagacat
     7141 cgccacccac gtctacgtcg cgatcctcac cgacaccggc tcgtttcact actcgaacat
     7201 cacgccgcgg accttcgaca tctgccggca gtgcatcgac gccggcgtca acccgtcgct
     7261 cgtcgcgcgg acgatcttcg acagcaacaa ccttggccgg ctcaagctgt tcggcgcggt
     7321 cctccatcag atgcagctcg acgcctcggg ccgcatcgcg acgctgtggg tggaccagaa
     7381 gctggcgcgc gactgcggcg gcacttacga ggacaccgaa ggcctggtca acctcccgct
     7441 cacggtcagg gacatccagg cggtggtctt cttcaaggag aacggcccgg gcgactggcg
     7501 cgtgagcttg cgatcgaagg gcgagatcga cgtcaacgcc atcgcgcggg agttcggcgg
     7561 cggaggccac gtgaatgcgt ccggatgctc ggcctccggt gcactcgccg atctgaggcc
     7621 ggtcttcgag aagaagctga cggaggccgt acagcagtgg atggcgtcct cgtcgtcgac
     7681 aagccgcagg ccgtaacctc ccacgacgtc gtcgccgagg cgcggcgccg gctgggccag
     7741 cctcgcctcg ggcacacggg aacactggat ccgcttgcca ccggcgtgct gccgctggcc
     7801 tgcggccggg ccacgcgcct ggtgcgattc ctgacggcgg cggacaagga gtacgaggcc
     7861 gggctccgct tcggggtgtc caccgactcc tacgacatca tgggccgcga gctcatgcgg
     7921 tccgggctga cgccgtcgaa agaggcggtc gagcgggcgc tcgcgtcgct gcggggtgag
     7981 tacctccagg tgccgccggc gcactcggcg aagagagtcg gcgggcggcg ggcgtacgag
     8041 ctggcgaggc gggacgaaga ggtgacgctc gcccctgtcc gcgtgcgcgt gacgcgggcg
     8101 gacctcgtga cgcttgacgg cgcctcggca atcgttgcga ttacctgttc gtccggcttc
     8161 tacgtccgca gtttcgcgca cgcgctcggc gagctcactg gcacgggagc ctgtgtcgaa
     8221 tcgctgcgcc gtacgcgtca cggcgagttc agcctgagcc aggccgtcac ggtcgagcaa
     8281 ctccggcagg gtgcggcggc ggtcggcgat cgctggattc cgctcgacag gctgctgccc
     8341 ggcctcccat cggtgcgtgt cagcgcggag gggcgaacgc gggtgtcgca cggacaggaa
     8401 ctgcagggct cgcacctgct cgacgaggcg gtcgaggggt ccgattcgtg gattcggctg
     8461 ctcgacgagg acaaccacct gctggccgtg gcgacggcct cgtccgcgcg tgctttgcac
     8521 ccggccgtgg tcctgattta actcaggaac tcgcttgtgt tggtggcatc ggcaacgctc
     8581 ttccctgcgg aacggccgcc tctttcgtcg caaccgtacg ccggggcttc aacacaagcg
     8641 agctgtggtg tagaatgaac tgtttaaggg ttcaaggatt gtcgagtctg tacggcggtc
     8701 cggtggatgg cccggccgtg tcggcccgtg gcgatcaggt ctggaccgtt cgtgaaggag
     8761 ccaccagtgg cgctgagcag ggaccggaag agcggcatca tcggaacata cagaacgcac
     8821 gacggcgata ccgggtcacc ggaagtccag gtggcactcc tcagcgagcg catcaactac
     8881 ctcaccgagc acttcaagat tcacgcaaaa gatcatcatt cccgccgtgg gctgctgaag
     8941 ctcgtcggtc agcgtcgtcg cctgctcgac tacctcaaga gcaaggactc cggacgctac
     9001 gccgaaatca tcgagcgcct cggcattcgt aagtagaacc aagtcacatg cacaacgttc
     9061 acctgcagac gcgcgagatc cggattggat cgcgcacagt gtccctcgag accggcaagc
     9121 tggccaagca ggccgacggt tcggtcctcg tccgatcggg cgatacggtc gtcctggtga
     9181 ccgcggccca cgccgcgagc gcacgggaag gcatcgactt tctcccgctg acggtcgact
     9241 atcgcgagta cacctacgcc tcgggccgaa tcccgggagg cttcttcaag cgcgagggca
     9301 agccgtccga aaaggaagtg ctgacgagcc ggctgatcga ccggccgatc cgcccgctct
     9361 ttacgtcggg ctggcgctac gagacgcaga tcatcgcgct gctcatgtcg gcggacaccg
     9421 acaacgacgc cgacgtgctg gcgatcacgg gcgcctcggc ggcgctcgcc ctgtcggaga
     9481 ttccgttcga gaagacgatc gccggcgtgc gcgtcggtat cgtggacggc gcgttcgtca
     9541 tcaacccgac cttcaccgag cgcaaggcca gcacgctcga cctcgtcgtc gccggcacac
     9601 gcgacggcat cgtcatggtg gaggcgggcg cgagggaagt ctccgaggcg caggtcgtcg
     9661 gagcgctcga ggccgcgcac gcggcgatca agacgatcgt cgccagcatc gacgacctca
     9721 aggcggccgc cggcaagaag aagctgacgg tccagaccaa ggccatcggc cacgagttct
     9781 accgcgaggt cgaggagaag gtctacgtcc cgctcggcga ggccatgcgc ctgaacggga
     9841 agctcgaaag ctaccagcgc gtggaccagg tgctcgaaga cctcatcgcc tcgattcccg
     9901 aaggcgagct ggagcgcaag gtcgaagcca agaagatctt cagcgagttg aaagagaagg
     9961 tcctgcgcga ctcggtgctc gacaagagcg tccggctcga tggccgcaag ttcgacgaga
    10021 tccggccgat ctggtgcgaa acgggcgtgc tgccgcggac gcacggatcg gtcgttttca
    10081 cgcgcggcga aacgcaagcg ctcgtcacct gcacgctcgg caccgccgac gacgagcaga
    10141 agatcgagca cgtggccggg gagttctaca agcgcttcat gctccactac aacttcccgc
    10201 cgttctcggt gggcgaggtc cagttcctgc gaggccccgg ccggcgcgaa gtgggccacg
    10261 gagcgctggc ggaacgcgcg ctcgctccga tggtccccag cgaagatgcc ttcgcctaca
    10321 cgattcgcct ggtctccgac atcctcgagt ccaacgggtc gtcgtcgatg gcgtcggtgt
    10381 gcggcggctc gatggcgatg atggatgcgg gcgtgccgct caagaatccc gtcgcgggaa
    10441 tcgccatggg cctgatcatg gacgaacagt ccggccgctg ggccgtgctg tcggacatcg
    10501 cgggcgccga ggatcactac ggtgacatgg acttcaaggt cgccggcacg gcgcacggga
    10561 tcacggcgct gcagatggac atcaaggtga ccggcatcac ctccgagatc atggccaagg
    10621 cgctcgagca ggcgcgggct ggccgcatgc acatcctcgg cgaaatggcc aagacgatcg
    10681 cggcgccgcg ccgggacatg tcgatgttcg cgccgaggat cgtcacgatc aagattccgg
    10741 tggacaagat ccgcgacgtc atcggcccag gcggcaagat gattcgcagc atcatcgaac
    10801 ggaccggcgt gaagatcgac gtcgaggacg acgggcgggt caacgtcgcc tccgccgacg
    10861 gagaatcggc cgccaaggcc atcggcatca tccaggagct gacggcgacg cccgaactga
    10921 acaagaccta catgggcaag gtgcagcgaa tcaccgattt cggcgcgttc gtggaaatca
    10981 tggcgggcac cgacggcctg ctccacgtct cggagatcgc caatcaccgc gtcaaggacg
    11041 tcagggacga actcaaggag gggcagcaga tcctcgtcaa ggtcatcaat atcgatccga
    11101 cgggcaagat ccgcctcagc cgcaaagctc tcttgcaaga ggaggcccag aaggccgcct
    11161 cggccccggc cgcgaaagag tagtgtccgg ctccagccgg accggcgtcc atagtccagc
    11221 ctgagcaatg gatgagaccg ggaccccctc ccggtcgtct acaattcaga catggtcctc
    11281 aagaagcgcg cagtccggct gctgcttgcg ggcatggccg gcctggcaat cggcccggct
    11341 ccgttcggcg tcgccggcca ggatcaggcg cccaaccggc gcgaaatcgc cctcacggcg
    11401 aagaactacc ggttctctcc cagccggatc gaggccgctc aggacgatct cctcaccctc
    11461 accgtgcaga gcgaagatgt ggcgtacggc ttcaccatcg atgagtatcg ggtgtcgagg
    11521 cgcgtccctg cgggcggcat ggccatgctt cagttccgcg ccgatcgccc cggaaagttc
    11581 atcttctact ccaacctcac caacgatgca cggcacgccc agatgaaggg cgagctcatc
    11641 gtcagagcac ggcagtagcc tagagcctgg agtccagctg ctttgccgct tcgatccgcg
    11701 cggcggtgga tgggtgcgtg tgaaacagca gctcgatcag gcgggacggc cgctcctcgg
    11761 cgaggttgcg gcccgccagc cgcttcatgg cgctgatgaa ggccgcctga ttccgcgtca
    11821 tctcgagcgc gtcacggtcg gccgttcgct cgtgcgcacg cgacaacgca ttggtcaccg
    11881 gcatcgaggc aaaggagacg gctcccaccg ccagcgcgac gagcggcaga gccgcaatgt
    11941 ccgacttgcc ttcgagatcg agcgcgggac caagcaccgt cagcgcggcg tcggcggcgt
    12001 agcagcccag ggcgatcagc ccggcctcga gcgcgatggc cgaccagacg tgcccccgcg
    12061 cgtcatgcgc gatctcgtgc gcgagcacgg cttcgatctc ctcgtccgag tgttcggcga
    12121 gcagggtgtc cgacaccagg atccgccggc cgctgccgat gcccacgagc gccgcgctcg
    12181 ccttgcgcgt ccggtctccc acccgccact cgaaaacgcc gagcacgcgc gcgccggccc
    12241 ggtcggccag cgccagcagg cgcgagccga gcggctcgcg cgcgagcggc ttcacgtcat
    12301 agaagaacgg cagcagaacg gccggcatca gcttcgccag cagcacgagg gtcgcggcaa
    12361 acgagacggc cgccgcgatc caccagcgat cgggactcca ccgaaggagc gacgagacga
    12421 gcagcgcgcc ggcgacgagg cagcccaggg tgatcgccga cgacgtgacg tgatccgtcc
    12481 accaccggcc ggtggtctct gtggacagac tgtagcgccg ctcgaggacg acgccgtgat
    12541 agaaggccag gggaagccga accagctcgt acaggacgcc gatcgccagc acgtagagaa
    12601 agcagacgat cgcgaatccg gggccgaccc gcgattcgat ggcgagccgc agggccgaag
    12661 agcccccggt caccagaatc gcgaccagaa gcacagcctg ccacgccgta ccgagcagga
    12721 acgtccgtcg gcgaaggcgt tgataccggg tggccttgtc ctcgttcgcc acctgtcgaa
    12781 ccctactggc gatcccggcg cgtggtgacg cgaccttcgc gagacccgat cgtgatgccg
    12841 ccgttggtga cgtgaaccac gattcgcggg ccgccgctgc cgatgcgccc gtcgacccgc
    12901 tgcggggtcc tctggtcggc cgcgaacgtc acgtcgtctg agatcctcac tccgccgttg
    12961 acgacggtcg cgtcgagctc ggcgttcacg cccggcccga cgttcatccg gacgccgccg
    13021 ttgaccgcgc tgaacctgga gtcgccggcg atcgactcga ggctgacgtc cactccgccg
    13081 ttgacggttg acgcgtccac acggccagac aactgccggc cggtcagagt cccgttggtc
    13141 gccgatgcgg tgacggtgcc ctgcagaccc tccatccgca catcgccgtt ctgcgtcctg
    13201 agcaggacgt ccaggcccgg cggaatccgc acctcgtagc ggatcgtgac ccgcgatcgc
    13261 gcctggccgc gccctccctc gccttcagcc a
//
LOCUS       DBNGSH010000874         2973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1127366, whole genome shotgun sequence.
ACCESSION   DBNGSH010000874 DBNGSH010000000
VERSION     DBNGSH010000874.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1127366"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC16CY_20085"
     CDS             <1..278
                     /locus_tag="AC16CY_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356162.1"
                     /translation="PLCAGCRTREARYGFRDDQEPSAIRPRTLCFDCFRMEIDWRQNV
                     AARLSRGWNAAPVRLPLEERLNELTRKRRRAQIAARHALETSPEPGA"
     gene            complement(275..1525)
                     /locus_tag="AC16CY_20090"
     CDS             complement(275..1525)
                     /locus_tag="AC16CY_20090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3356163.1"
                     /translation="MIAREESLEDAIESLVQAGQLAGAAALVWRDGTATDVVTVGRRD
                     LVSGLPVERDTIFRLASLTKPVTTVAALSMLDEGRFTLDEPIAGVAPELARLRVLRAP
                     EGPLDQCDDAARPITFRDLLTHRSGLTYADFHSGPIGRAYANVLGPQIDNPLTPDEWI
                     ARLATLPLIDQPGTGFHYGHSTDLLGFLLARLEGAPLGAVLERRVFAPLDMSDTGFAV
                     PPAQRDRRAGLCGFDGEGRMATLTAAPGGHALPDRPEGMTFESGGQGLWSTLDDFLTF
                     ARTLIGDGVPGAPLLGRETLAMMTSNQLTPDQRAATRLLGQPIFAAGHGYGMGLAVVM
                     EPEAADPLRCRGGVGTIGWPGAYGAWWQADPNDGSVLIFLAHNMVELNQMAQGIGLGV
                     WSAIGRFHEIATRPAITRRNDLPR"
     gene            complement(1522..>2973)
                     /locus_tag="AC16CY_20095"
     CDS             complement(1522..>2973)
                     /locus_tag="AC16CY_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356164.1"
                     /translation="DARRPSHARADDERRQDRKRAHAMIGRLAFRSLTAHPVRSAVLA
                     AGFGAGVAVMALLLGVASIVLEQAQSPALVGGGDVNISLPLGVPARLVLAGTLQSNTM
                     RPRVRAAAASHTTELYLLRDGRRTRVDVRGGIPSVERELGDPEVAGIGAWRDTPEDSA
                     WTREPPDVVLRQIDRFHGVPDVPEWADSWAEWLYFNGRSKEARFYLTFLVGPRTKSGN
                     RAAGVRLQIERNGRMESYGASTEVTDADLARAPDLAIGASSVRLDGMVYRIHVDVTGA
                     GGRRATGDLSLTASAGRFVPPIEITGARGWRTGYVVPVMSGALDGALTVGPDEIPLGG
                     GTGYHDHNWGFWRGVSWQWGQAQQGDLSLIYGRVFPPPEAADPERIPGLVGALGPDGP
                     LGYATDVRITETNDASGRPQTINVRARGPALTLDLRFDVTSTETSRMTQGPLGNGLDF
                     LQMRGQYTVSGRAGERELSFTAPGSAETFRGDQ"
BASE COUNT          467 a         1062 c         1002 g          442 t
ORIGIN      
        1 cgccgctctg tgccggttgc cggacgcgcg aagcgcgcta cggcttccgt gacgatcagg
       61 agccgtcggc gatccgtccc cgcacgctgt gcttcgactg cttccgcatg gagatcgact
      121 ggcgtcaaaa cgtcgcggcc cggctcagtc gcggatggaa cgcagcgccg gtgcggctgc
      181 cgctcgagga gcggctcaac gagctgaccc gaaagcgccg ccgcgcgcag atcgccgcgc
      241 ggcatgcgct ggaaacgagc cccgagcccg gagcctaacg cggcagatcg ttgcggcgtg
      301 ttatggctgg acgcgtggcg atctcgtgaa accggccgat ggcgctccag acgcccaacc
      361 cgatcccctg tgccatctgg ttcagttcca ccatgttgtg cgcgagaaag atcagcaccg
      421 acccatcgtt cggatccgcc tgccaccagg cgccgtaggc gcccggccat ccgatcgtcc
      481 ccacgccgcc acggcaacgc aacggatctg cggcctccgg ttccatgacg accgcgaggc
      541 ccataccata gccgtgaccg gcggcaaaga tcggctgtcc gagcagacgc gtcgccgcac
      601 gctggtccgg cgtcaactgg ttcgacgtca tcatcgccag cgtctctcga ccgagaagcg
      661 gcgctcccgg cacgccgtcg ccgatgagcg ttcgcgcaaa cgtcagaaag tcgtcgagcg
      721 tagaccacaa accctggccg cccgactcga acgtcatccc ctccggccga tcgggcagag
      781 cgtggccacc cggtgcggca gtcagcgtag ccatgcggcc ctcgccgtcg aagccgcaca
      841 acccggcgcg gcgatcacgt tgtgccggcg gtaccgcaaa gccggtgtcg gacatgtcga
      901 ggggtgcgaa gactcgccgc tccagcacgg cacccaacgg ggcgccctcg aggcgcgcca
      961 gcaggaatcc gagcagatcg gtggagtggc cgtagtggaa gccggtgcca ggctggtcga
     1021 tcagcggcag cgttgcgagg cgtgcgatcc attcatccgg ggtgagcgga ttgtcgatct
     1081 gggggcccag cacgttcgca taggcgcgcc cgatcggccc gctgtggaaa tcggcgtagg
     1141 tcagcccgga acgatgcgtc agcagatcac ggaaggtgat tggacgcgcg gcgtcgtcgc
     1201 attgatcgag aggtccctcg ggagcgcgca atacgcgcag acgcgcaagc tccggggcga
     1261 ctcctgcgat cggctcgtcg agcgtaaagc gcccctcgtc cagcatcgaa agcgcggcca
     1321 ccgtcgtcac gggtttcgtc agcgaggcga ggcggaagat cgtgtcgcgt tcgacgggca
     1381 gtcccgagac gaggtcgcgg cgtcccaccg tgacgacatc cgtggcggtt ccgtcccgcc
     1441 aaacgagtgc agccgctccg gccagctggc cggcttggac gagagactca atcgcgtctt
     1501 ccagagactc ttcgcgtgcg atcactggtc ccctcgaaac gtctcggccg atcccggggc
     1561 ggtgaacgag agctcccgtt cgcccgcgcg gcccgacacc gtgtactggc cgcgcatctg
     1621 cagaaagtcc aggccgttgc cgagcgggcc ttgcgtcatt cgagacgtct ctgtggacgt
     1681 cacgtcaaat cggaggtcga gcgtcagcgc tggtccgcgt gcgcggacgt tgatcgtttg
     1741 cgggcggccg ctcgcgtcgt tggtttccgt gatgcgcacg tcggtggcgt agccgagggg
     1801 gccgtccggt ccgagcgcgc ccacgaggcc cgggatccgc tccgggtcgg cggcctccgg
     1861 cggtggaaac acgcgtccgt agatcagcga cagatcgcct tgctgcgcct gtccccactg
     1921 ccacgacacg cctcgccaga atccccagtt gtggtcgtga tagcccgtac ctcccccgag
     1981 cgggatctcg tccggtccca ccgtcagggc tccgtcgagc gcgcccgaca tcaccggaac
     2041 gacgtagccc gtacgccagc cgcgcgcccc ggtgatctcg atcggaggca cgaagcggcc
     2101 ggcggatgcc gtgagcgaca ggtcgccggt tgcgcggcgc cccccggcgc ccgtgacgtc
     2161 cacgtgaatg cggtacacca tgccgtcgag ccgtacggag ctggcgccga ttgccaggtc
     2221 cggcgcccgc gcgagatcgg catcagtgac ttccgtgctc gcgccgtacg actccatgcg
     2281 gccgttccgc tcgatctgca gcctgacgcc cgcagcgcgg tttcccgact tcgtgcgggg
     2341 accgacgagg aacgtcaggt aaaagcgcgc ctccttcgaa cggccgttga agtacagcca
     2401 ctcggcccag gagtcggccc actcgggcac gtcgggcacg ccgtggaagc gatcgatctg
     2461 ccgcagcacc acgtccggag gctcgcgcgt ccacgccgag tcctccggcg tgtcccgcca
     2521 ggcgccgatg ccggcaacct ccgggtcgcc gagctcccgt tcgacgctcg ggatcccgcc
     2581 tcgcacatcg acgcgcgtac ggcggccatc gcgaagcaga tacagctccg tcgtgtgtga
     2641 cgccgccgcg gccctcacgc gcgggcgcat cgtgttggat tgcagcgtgc ccgcgagcac
     2701 gagccgtgcc ggcacgccga gcggcaggga gatgttcacg tcgccgccgc cgacgagcgc
     2761 cggcgactgc gcctgctcga gcacgatgct cgccacgccg aggaggaggg ccatcaccgc
     2821 cacgccggcg ccgaagcccg ccgccagcac ggcgctccgc acgggatggg ccgtcagcga
     2881 tctgaacgcc agcctgccga tcatcgcgtg cgctcgcttt cgatcctgcc gtctctcatc
     2941 gtcagcacgc gcgtggctcg gtcggcgagc gtc
//
LOCUS       DBNGSH010000875         7133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_958660, whole genome shotgun sequence.
ACCESSION   DBNGSH010000875 DBNGSH010000000
VERSION     DBNGSH010000875.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_958660"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1459)
                     /locus_tag="AC16CY_20100"
     CDS             complement(287..1459)
                     /locus_tag="AC16CY_20100"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3356165.1"
                     /translation="MTSMTSVATTVDLPSRAKAVATIADECAEAGDRHGLLSTPVVEA
                     LHREGLLGMWVPRSLRRGAELDPVSSLEVIEHLSYGDPSAGWVLMASALAIGTGASYL
                     GDAAVAELFGGDRLPVIAGQGTRPGNAVPQAGGFRLTGSWSFASGIKHATHIHTLGVI
                     EGTGEPRIFVLPVASATLIENWDVLGLRGTGSIDYRTEGVFVPEAYTHFAVTESPRRG
                     GALFTIGIAGFALICHSGWACGIGRRLLDELAAKVRSGIGRSGTLAGSDAFHEQYAKA
                     EGKYRAARALVYETWSDVKATLDRGAALDTRQRTLTRLALSHITWCCHDVADFVYSAA
                     GTVALRAGTIQRLVRDMYAGSQHITSAPPVIRNTGRELAGLADRKKWVFLDLVDEI"
     gene            complement(1619..4744)
                     /locus_tag="AC16CY_20105"
     CDS             complement(1619..4744)
                     /locus_tag="AC16CY_20105"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3356166.1"
                     /translation="MRGVMNALVIAAALVVLPTLAHAQGTLTGTVRDASGAVLPGVTV
                     EASSDALIEKVRSVVTDGTGQYRIIDLRPGTYALSFTLPGFTAVKREGVVLAGSQTLT
                     IPIEMRVGQLQETITVTGETPVVDLQSVKRELVLDSEVIQAIPATRAVGSLLNATPGL
                     TVDNNGMAASPTMTFFSARGGQANEGRMTINNMIVAAAFNGGGVSSYIYDSPNSDEVS
                     VVVSGGLGEADIGGPIMNLVPRTGGNSFRGQAFFNTAGDWSRGNNLTEDLMAPPPGPN
                     LRETPGIIQSYDGSASYGGPIMRDRLWFFGSYRKLETHTAVEGVRANANVYDASRWDW
                     LESPVTARFAQGRVMYIGRLTAQVTERNRVSFNYEYQHRCEGTPLKLETTGACRSRSA
                     DWIGLGTTTQSPEATTTAARGYFDFPYHVTQGMWTAPLTNKLLAEAGYTSFIYWPAFG
                     QAPPDGIHNLIPVSEQSTAVNPATGGQYAPAANYAYRGLLSAGRNYGNPNNWRGSLSY
                     VTGAHNMKVGYQGAYFITKDHLVTNDSLMSYRFNQGQPNRVTYRLPEWEAANRVMSNS
                     LYIQDTWTRGRLTLQGALRYDRASSFSPADGNGTQETSPFNAAPITFSRVEGVNAYND
                     LTPRVGVAYDLFGNGKTAIKFNWGHYLDAATNDSIYTAMNPANRIIQSVDRDWTDTNG
                     NKQVDCNLMDPALQTAIDRCAALTGNALNFGQTNPSFATVNPGLLHGWGVRENDYQWG
                     INVQQEIVPRVSLDVGYNRRWFHGATVSDNINRSPDEWDSWVINAPLDPRLPEGGGYP
                     ITMYTVKAANATEPAQNNTTFETDFGPERINYWHGVDVTVNARLQNGLMVQVGTSTGR
                     GVFDACAATTKMDTPDPRTSVPGFGGGQNCKDIDPVETTFRGLASYTIPKIDVLVSAT
                     FRSQPTQQLTATWSIPNALVVAALGRLPVGANPTGNTAVALLDNEHRVYDEDRRAQID
                     MRFAKIFRFGGTRTNVGVDIGNLLNTNYGTAFENTYQYSVGNTAMGGTWANPTAIYTP
                     RFFRLNFTVDF"
     gene            5004..5777
                     /locus_tag="AC16CY_20110"
     CDS             5004..5777
                     /locus_tag="AC16CY_20110"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3356167.1"
                     /translation="MPKIPALVPQDRQGRTADVASQIARAFVGLREMLLVGVFGRGER
                     ISELPLVARLGMSRTPIRLALERLAHIGFLDVVPTGGFVVRGFTIAEVLDAIEVRAVL
                     EGTAARLAAERLTDVSELDPLRQYSAKMGALKGLTIDSLSDYMDLNEAFHAALVDLSK
                     SAMLRRSIEQVNSLPFASPSAMVFPTSVLPNSDETLAIANLHHRSIIEAVSHRQGTRA
                     ESLAREHTFIARRVLELALSDANALKRVPGGALINVSAT"
     gene            complement(5774..6040)
                     /locus_tag="AC16CY_20115"
     CDS             complement(5774..6040)
                     /locus_tag="AC16CY_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356168.1"
                     /translation="MRFFQSTSQTRLAVGDPGQSKEAIFEVTGKKGTVLQVFNRLSLG
                     VQHPFYALDDVLAMGQEELKKLDVFVKRHLARTRHALDRKPRGG"
     gene            6287..>7133
                     /locus_tag="AC16CY_20120"
     CDS             6287..>7133
                     /locus_tag="AC16CY_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356169.1"
                     /translation="MLDQLWKAAPPDQPVLVRDARWLIQLAQSPATDELGAYFEVAEE
                     IAEAFSPEDRVEIHKASARMAAGHLRSQIRYHSLKKAVSLDDKSLVLSTRNSNALDFA
                     LLVHDLVPLLEAYEIAWQSGDGQKRVELADAICQGVSPDPELFLNRVELLGAYTMIEH
                     LFTATERDGHAVYTAMGTRHVQLLQEYESRIGRVSKPLYEDCPRFRPAAGAYSPYGVL
                     YGFSTDLIEHMALKTLQPDTVTHLGLEDVFVDGDASTGKLAWVSGWRKLPHLTPEVEK
                     LFDYPQ"
BASE COUNT         1172 a         2280 c         2387 g         1294 t
ORIGIN      
        1 gggccggggg gacacgggct cgagacgatc cgcgcgagcg ggatgtaacg tccttggtgc
       61 ttcgtccttc gttcttggtc cgtccctggt cctgggtccg tccttggtcc tcggtcacga
      121 agtctgagcc cgaacggtat cgctcacaga cggtgagccg gatggtcgca ccggggacca
      181 aggactaagt aaggacaacg gacggaccaa ggacaacgga cggaccaagg acttaggacg
      241 gaccaagtac caagaacaaa gcaccaagga ctaaagtggc cgcggatcag atctcgtcca
      301 cgagatcgag gaacacccac ttcttcctgt cggccaggcc ggcgagctcg cggccggtgt
      361 tgcggatgac cgggggcgcc gatgtgatgt gctggctgcc cgcgtacatg tcgcggacga
      421 gccgctggat cgtgccggcg cgcagcgcga ccgtgcccgc ggcgctgtac acgaagtccg
      481 ccacgtcgtg gcagcaccac gtgatatgtg acagagccag ccgcgtgagc gtgcgctggc
      541 gcgtgtcgag cgccgcgccc ctgtcgagcg tggccttcac gtcgctccag gtttcgtaga
      601 ccagcgcgcg cgcggcacga tacttccctt cggccttcgc atactgctcg tggaaggcgt
      661 cgctgcccgc cagcgtgccg gagcggccga tgcccgagcg gaccttcgcc gccagctcgt
      721 cgagcaggcg ccggccgatg ccgcaggccc accccgagtg acagatgagg gcaaagccgg
      781 cgatgccgat cgtgaacagc gccccgccgc gtctcggcga ctcggtgacg gcaaagtgcg
      841 tgtacgcctc ggggacgaac acgccctcgg tccgatagtc gatgctgccg gtcccgcgca
      901 ggccgagcac gtcccagttc tcgatgagcg tcgcgctggc cacgggcagc acgaagatcc
      961 ggggctcccc ggtgccctcg atcacgccga gcgtgtgaat gtgcgtggcg tgcttgatac
     1021 cggaggcgaa gctccacgaa cccgtcagcc tgaagccgcc cgcctgcggg acggcgttcc
     1081 cgggacgagt gccctgcccc gcgatgacgg gcaggcgatc gccgccgaac agctcggcga
     1141 cggccgcatc tcccaggtag gaggcgccgg tgccgatggc aagagccgac gccatcaaca
     1201 cccagccggc cgatggatcc ccgtaggaca ggtgctcgat gacctcgagc gaggagaccg
     1261 ggtcgagctc ggcgccgcgg cgaagcgacc gcggcaccca catcccgagc agcccctccc
     1321 ggtgcagcgc ctcgacgacc ggcgtcgaca gcagcccgtg ccggtctcct gcttccgcgc
     1381 attcatccgc gatcgtcgcg accgccttcg ccctcgaggg gagatccacc gtcgtcgcca
     1441 cgcttgtcat gctcgtcatt ccgtctgctc cacggccgag ttgtacacgg cgagtgaagg
     1501 ggaatgatat cgctggaggc cgctgggctg aacggtcgag gaaaggaggc ctcccaaccc
     1561 ccaagattgg ccttgggagt tgggagttgg aagttgggag ttaacgcaaa agctctgact
     1621 agaagtccac cgtgaagttc aggcggaaga accggggcgt gtagatcgcc gtcgggttcg
     1681 cccacgtccc gcccatcgcg gtgttgccaa cgctgtactg ataggtgttc tcgaacgccg
     1741 tcccgtagtt cgtgttcagg aggttgccga tgtccacgcc gacgttggtg cgggtccccc
     1801 cgaagcggaa gatcttggcg aagcgcatgt cgatctgcgc ccggcgatct tcgtcgtaga
     1861 cgcgatgctc gttgtcgagc agcgccacgg ccgtgttccc ggtcgggttt gcgccgaccg
     1921 gcagccggcc gagcgcggca acgacgagcg catttggaat gctccaggtg gccgtcaact
     1981 gctgcgtcgg ctgggagcgg aacgtcgcgc tgaccagcac gtcgatcttc gggatcgtat
     2041 acgacgccag gccccggaag gtcgtctcga ccggatcgat gtccttgcag ttctggccgc
     2101 ccccgaaacc cggcacagag gtccggggat ccggcgtgtc catcttggtg gtggcggcgc
     2161 aggcatcgaa gaccccgcgc cccgtgctcg tccccacctg caccatcagg ccgttctgca
     2221 ggcgcgcgtt cacggtgacg tcaacgccgt gccagtagtt gatgcgctcg ggcccgaagt
     2281 cggtttcgaa ggtcgtgttg ttctgcgccg gctcggtggc gttcgccgcc ttgaccgtgt
     2341 acatcgtgat cgggtagccg ccgccctccg gaaggcgagg gtcgagcggc gcgttgatga
     2401 cccagctgtc ccactcgtcc ggagatcgat tgatgttgtc gctcaccgtt gcaccgtgga
     2461 accagcggcg gttgtacccc acgtcgagcg acacgcgcgg gacgatctcc tgctggacgt
     2521 tgatgcccca ctggtagtcg ttctcgcgga cgccccagcc gtgcagcagc cccgggttga
     2581 cggtggcaaa gctggggttg gtctggccga agttcagcgc gttgccggtc agcgcggcgc
     2641 atctgtcgat cgccgtctgc agggccggat ccatgagatt gcagtccacc tgcttgttgc
     2701 cgttggtgtc ggtccagtca cggtccacgc tctggatgat gcggttggcg gggttcatcg
     2761 ccgtgtagat gctgtcgttg gtcgccgcat cgaggtagtg gccccagttg aacttgatcg
     2821 ccgtcttgcc gttgccgaac aggtcgtacg cgacgcccac gcgcggagtg aggtcgttgt
     2881 aggcgttgac gccctcgaca cgggagaagg tgatcggcgc ggcgttgaac ggcgacgtct
     2941 cctgggtgcc attgccgtcc gccgggctga agctcgaggc gcggtcgtag cgcagcgcgc
     3001 cctgcagcgt cagccgcccg cgcgtccagg tgtcctggat gtacaggctg ttcgacatca
     3061 cccggttagc agcttcccac tccggcaggc gataggtgac ccggttcggc tggccctggt
     3121 tgaagcggta ggacatcagt gaatcgttcg tgacgaggtg gtccttcgtg atgaagtacg
     3181 caccctggta gccgaccttc atgttgtgcg ccccggtcac ataggagagc gaaccccgcc
     3241 agttgttcgg gttgccgtag ttgcgtccgg cgctcagaag tccgcgatag gcgtaattgg
     3301 cggccggggc atactgcccc ccggtggccg gattcaccgc ggtggactgc tcactcaccg
     3361 ggatgaggtt gtggatgccg tcgggcggcg cctggccgaa cgccggccag tagatgaagc
     3421 tcgtgtaccc cgcctcagcc agcagcttgt tcgtgagcgg cgctgtccac atgccctgcg
     3481 tcacgtggta cgggaaatcg aagtagccgc gcgccgcggt cgtcgtcgct tccggcgact
     3541 gtgtcgtcgt gccgagcccg atccagtccg cggaacgtga acggcaggca ccggtggtct
     3601 cgagcttcag cggcgtcccc tcgcagcgat gctggtactc gtagttgaac gagacacggt
     3661 tccgttccgt gacctgtgcg gtgaggcggc cgatgtacat cacgcggccc tgcgcgaatc
     3721 gcgcggtcac cggggactcg agccagtccc atcgcgaggc gtcgtagacg ttggcgttcg
     3781 ccctgacgcc ctcgacggcc gtatgcgtct cgagcttccg gtagctgccg aagaaccaca
     3841 gccgatcccg catgatggga ccgccgtagg acgcgctgcc gtcgtaggac tggatgatgc
     3901 ccggcgtttc cctcaggttc ggaccgggcg ggggtgccat cagatcctcg gtcaggttgt
     3961 tgcccctgga ccagtcgccc gccgtgttga agaacgcctg cccgcggaag gagttgccgc
     4021 cggtccgcgg aaccaggttc atgatgggac cgccgatgtc ggcttcgccg aggccgcccg
     4081 agacgacgac cgacacttcg tcgctattgg gcgagtcgta gatgtaggag gacacgcccc
     4141 cgccgttgaa cgcggcggcc acgatcatgt tgttgatcgt catgcgtccc tcgttcgcct
     4201 gcccgccgcg cgcgctgaag aacgtcatcg tcggcgacgc cgccatgccg ttgttgtcca
     4261 cggtgagacc gggcgtcgcg ttgaggagcg acccgaccgc gcgcgtcgcc ggaatggcct
     4321 ggatgacctc ggagtcgagc acgagctcac gcttcacgct ttgcaaatcc accaccggcg
     4381 tctcgcccgt gacggtgatc gtttcctgga gctgcccgac ccgcatctcg atcgggatcg
     4441 tcagcgtctg ggagccggcc agcacgacgc cctcgcgctt gaccgcggta aagccgggca
     4501 gcgtgaatga cagcgcgtag gtgccggggc gtagatcgat gatgcggtac tgtccggtcc
     4561 cgtcagtcac gacggaacgg actttttcaa tcagggcgtc gctcgacgcc tcgacggtaa
     4621 cgccgggcag gacggcgccg gacgcgtccc ggacggtgcc ggtcagtgtg ccttgcgcgt
     4681 gcgcaagagt cggcagcacg acgagggctg ccgcgatgac caacgcgttc atcactccac
     4741 gcataccttc tccttgtctg aatcgtcact accgatgccc cccgccgttg aggccggtcg
     4801 gccattccgc ggggggactc actggcatgc cgcctcggcc ggcctgcatg cagatgggcg
     4861 gagaatacgc atggcacact gatatgtcaa actgaatcgc attgataatc gatatatgtg
     4921 agatcgcagg ttccatgtcc tgcatgcaag acgctgcact ggctgatgtt ctgcatgcgg
     4981 ccgtgctgca gtaggattca agaatgccga agattccggc actcgtcccg caggaccgcc
     5041 aggggcggac ggccgacgtc gcctcgcaga tcgcgcgggc gttcgtcggg ctgagagaga
     5101 tgctgctggt cggcgtgttc gggcgcggcg agcgcatctc ggagcttccg ctcgtggcgc
     5161 ggctcggcat gtcgaggacg cccatccggc tcgcgctcga gcgtctggcg cacatcggct
     5221 ttctcgacgt cgtccccacc ggcggcttcg tggtgcgggg gttcacgatc gccgaggtcc
     5281 tcgatgcgat cgaagtacgc gccgtgctgg aaggcaccgc ggcgcgcctg gcggccgaga
     5341 ggctcaccga cgtgtcggag ctcgatcccc ttcgtcagta cagcgcgaag atgggagcgc
     5401 tcaaggggct gacgatcgac tcgctgtccg attacatgga cctgaacgag gcgtttcacg
     5461 cggcgctggt cgatctgtcc aagagcgcca tgctgcgccg cagcatcgag caggtgaact
     5521 cgctgccgtt cgcctcgccg agcgcgatgg tgtttccgac gtcggtcctg ccgaactcgg
     5581 acgagacgct cgcgatcgcc aacctgcatc atcgatcgat catcgaagcg gtcagccatc
     5641 ggcagggcac gcgggccgag agcctcgccc gcgagcacac cttcatcgcc cgccgcgtgc
     5701 tcgagctggc gctctcggat gccaacgcgc tgaagcgcgt gcccggcggc gcgctgatca
     5761 acgtctcggc gacttagccc ccccttggct tacgatccag ggcgtgccgc gtcctggcca
     5821 ggtgccgttt cacgaacaca tcgagcttct tcagttcttc ctggcccatc gcgaggacat
     5881 cgtcgagcgc atagaagggg tgctgaacgc ccagcgaaag ccgattgaat acctgcagga
     5941 cggttccctt ctttcccgtc acttcgaaga ttgcttcttt actctgaccg ggatcaccca
     6001 cagccaatcg cgtctgagag gtcgattgga agaagcgcat tgggccagtg gattcaggcc
     6061 tcgagagctt ccgggactgc acaacggcct ggtcgatccg gcggagatga tgatccgcgg
     6121 ctttcacttg tggcggcaca cgcgctggcc tggccgcaac ggacggctcc gttatgcgca
     6181 cacgcttttc aacctgtacg tcatccggtg cctcgagctg ttgagcatgc ggctatggga
     6241 cgcgggatcg ggcagcgccg gcgatcggct ctcgcaggtt caaggcgtgc tcgaccagct
     6301 ctggaaagcc gcgccgcccg atcagccggt gctcgtgcgg gatgcacgat ggttgattca
     6361 gctggcgcag agccccgcga cggatgaact gggggcctac ttcgaagtgg ccgaagagat
     6421 tgcagaggcc ttttcgccag aggaccgggt tgaaatacac aaagccagtg ctcgaatggc
     6481 ggccgggcac ttgcgctcgc agattcgcta ccactccttg aagaaggcag tgtctctcga
     6541 cgacaagagc ctggtgctga gcactcgcaa ctccaacgct cttgatttcg ccctgctcgt
     6601 tcacgatctc gtgccgctgc tcgaggcgta tgaaatcgcc tggcagagtg gggatggtca
     6661 gaagagggtc gaactggcgg acgccatttg ccagggcgtt tctccggacc cggagttgtt
     6721 cctgaatcgc gtcgaactgt taggcgcgta cacgatgatc gagcatctgt tcacggcgac
     6781 ggaacgcgat gggcacgcgg tgtatacggc catgggaacg cggcacgttc agctgctcca
     6841 ggagtacgaa tcacggatcg gccgcgtgtc gaagccgttg tatgaggatt gtccgcgctt
     6901 ccggccggca gccggcgcgt attcgccgta tggggtcctg tacgggttct cgaccgacct
     6961 catcgagcac atggcgctca agactttgca gcccgatacc gtgacgcacc tcggcctgga
     7021 agatgtcttt gtcgatgggg acgccagcac tggcaaactc gcgtgggtga gcggctggcg
     7081 gaaattgccg cacctcacgc cggaagtgga aaagctgttc gactaccccc agc
//
LOCUS       DBNGSH010000876         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_108738, whole genome shotgun sequence.
ACCESSION   DBNGSH010000876 DBNGSH010000000
VERSION     DBNGSH010000876.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_108738"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="AC16CY_20125"
     CDS             complement(<1..764)
                     /locus_tag="AC16CY_20125"
                     /inference="COORDINATES: protein motif:HMM:NF024821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356170.1"
                     /translation="MVSLAAALALTIVFQSPTERDQAEDLARAGRTQEAIAIYRRILE
                     QNPADTDARLWIARLNMRIGKTEDAEAAFRSVVREHPGDVDARIGLGAALTRRGAWDE
                     AIAILHETEPAAGQNADLFGTLARAYRRAGDDRSALEYFERARALTPTDWDIVSGYEA
                     TAQVYGHSIVFDGFTESGSTGTDAQSGSLSMLVRAAPRLHIHARGRVQQRAGTTDVLG
                     GGGVLWRAGRATTVGLYIAGGSGNTSLPTTDMWGEVV"
     gene            complement(967..>2543)
                     /locus_tag="AC16CY_20130"
     CDS             complement(967..>2543)
                     /locus_tag="AC16CY_20130"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3356171.1"
                     /translation="LAVLCVACANVAGLLASRAPERAREIALRLSIGASRPRIITGLL
                     AESLAIALVGGAAGVGVGYAGIVLLRQIEWPSELIVYPVIQLDERTMMFALLAALGSV
                     FLFGVGPAIQTARVDLARAMKANDASQSKRLRLRGRNVLVATQVAMSLAVLTIATFVF
                     QIFQRELTAGPGFRTSHIAMARIDPMQARYTGEGSLRFFEQAVEQARRLPRVVSATVA
                     SEIPMWGLELTSMVPEGYRLADGQSGVRIYSSSVDESFFATLGIAILSGRPFRATDAA
                     GSPKVAIVNEVIAARYWPGQDAVGRRFQLKDARGPWVEVIGVAKLSTYVYTGERPFEV
                     VYFPFRQEPRGAMMLLAHTSGDSRDLLDPLREMVRRMDPDVPISDAQTMERFYEVRAT
                     SINGIAVSLIGGLGIMGLILAMVGLYGLVSYAVGRRTREIGVRIAVGASPYRVLGMIL
                     QEGMRPAWVGLAAGLVLSIVTARLLPALVPIGHRYDAATVLAVVPLLLAVSTLAAGIP
                     ARRAARVDPTVALRTE"
BASE COUNT          441 a          893 c          848 g          361 t
ORIGIN      
        1 acgacctcac cccacatgtc cgtcgtcgga agcgaggtat tccccgagcc gcccgcgatg
       61 tacaggccca ccgtcgtcgc gcggccggcg cgccacagca cacccccgcc gcccaggacg
      121 tcggtcgtgc cggcgcgctg ctgcacgcgg ccgcgcgcgt ggatgtggag ccgcggcgcg
      181 gcgcgcacga gcatcgacag cgaacccgat tgagcgtccg ttcccgtgct gccgctctcg
      241 gtgaacccat cgaagacgat cgaatggccg taaacctgcg ccgtcgcttc gtagcccgac
      301 acgatgtccc agtcggtcgg cgtcagggcc ctggcccgct cgaagtactc gagcgcggat
      361 cggtcgtcgc cggcacgccg gtaggcacga gcgagcgtgc cgaacaggtc cgcgttctgc
      421 ccggccgccg gctccgtttc gtggaggatg gctatcgcct cgtcccaagc gccgcgacgc
      481 gtcagcgccg caccgaggcc aatgcgcgca tcgacgtcgc caggatgttc gcggacgacc
      541 gaacgaaagg cggcctcggc gtcctcggtc ttgccgatcc gcatgttcag ccgtgcgatc
      601 cacaaccgcg cgtccgtgtc ggccggattc tgctccagga tgcgccgata gatggcgatc
      661 gcctcctgcg tccgccccgc gcgagcgagg tcttcggcct gatcccgttc ggtgggggac
      721 tgaaagacga tcgtcagggc cagcgccgcg gcgagagaga ccataggaac ccgtgaaccg
      781 tgctgtggcg cttggacgaa ccgcgtgcga gtgtagcacc cactcacggt ccggctaaag
      841 ccggacacta cgtactcgcg gtagggccgg acactacgta ctcgcggcag ggccggacac
      901 gacgtactcg cggtagggcc ggacacgacg tactcccggt agggaacgtg agaagcggtc
      961 cgctccttac tccgtgcgca gcgcgaccgt gggatcgact cgcgcggcac gccgggcggg
     1021 gatgccggcc gcgagtgtgc tgactgccag aagcaacggc acgacggcca gcaccgtcgc
     1081 cgcatcgtag cggtgtccga tggggaccag cgccggcaga agccgcgccg tgacgatgct
     1141 caggaccaga ccggcggcga gcccgaccca ggcaggcctc atcccttcct gcaggatcat
     1201 cccgaggacg cgatacgggc tggctccgac ggcaatgcgc accccgatct cgcgcgtccg
     1261 ccgaccgacc gcgtacgaga cgagcccata gaggccgacc atcgcgagga tcagccccat
     1321 gatgccgaga ccgccgatca ggctgacggc gatcccgttg atgctggttg ctcgcacttc
     1381 gtagaaacgc tccatcgtct gcgcatccga gatgggcacg tctgggtcca tgcgacgcac
     1441 catctcccgc agcggatcga ggaggtcgcg ggagtctcct gaggtatgcg cgagcagcat
     1501 catcgccccg cgcggctcct gtcgaaacgg aaagtacacc acctcgaacg ggcgctcgcc
     1561 cgtgtagaca tacgtggaca acttcgcgac gccgatcact tcgacccacg gcccgcgcgc
     1621 atccttcaac tggaagcggc gtccgacggc gtcctgccct ggccagtagc gcgcggcgat
     1681 gacttcgttc acgatcgcca ccttcgggga ccccgcagcg tcggtcgctc ggaacggccg
     1741 ccccgacaga atggcgatgc ccagcgttgc gaagaaggac tcgtccacgc tgctcgagta
     1801 gatgcgcact cccgattgac cgtcggccag ccggtagcct tccggaacca ttgacgtcaa
     1861 ctccagcccc cacatgggta tctccgaggc gaccgtggcg gagacgacgc gaggaagacg
     1921 gcgggcctgc tcgacggcct gttcgaagaa tcgcagcgat ccttctccgg tgtagcgcgc
     1981 ctgcatcgga tcgatcctgg ccatcgcgat gtgcgacgtg cggaagccgg ggccggcggt
     2041 caactcgcgc tggaagatct ggaacacgaa tgtggcgatg gtcagcacgg cgagcgacat
     2101 cgcgacctgc gtggccacga gcacgttccg gccgcgcagc cggagccgct tcgactggct
     2161 cgcatcgttg gccttcatcg cccgggcgag atcgacgcgt gcggtctgaa tcgccgggcc
     2221 cacaccgaag agaaacacgc tgccgagagc cgccagcagg gcgaacatca tcgtgcgctc
     2281 gtcgagctgg atgacgggat acacgatcag ctcagagggc cattcgatct gtcgaagcag
     2341 gacgatgccg gcgtagccga cgccgacgcc cgccgcccct ccaacgagcg cgatcgcaag
     2401 gctctcggcg agcaaaccgg tgatgatccg cggccggctg gctccgatcg agagccggag
     2461 ggcgatctcg cgcgcgcgct ccggcgcccg gctggcgagc agcccggcca cgttcgcgca
     2521 cgcgacgcag aggaccgcga gcg
//
LOCUS       DBNGSH010000877         2931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3504747, whole genome shotgun sequence.
ACCESSION   DBNGSH010000877 DBNGSH010000000
VERSION     DBNGSH010000877.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3504747"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="AC16CY_20135"
     CDS             complement(<1..1431)
                     /locus_tag="AC16CY_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HIR3356172.1"
                     /translation="MATQSRPDAEARTAQQPVEQRPAEAAEAFVRALVAQDVECLFLN
                     PGTDTISIQEALSKMEQLGQPVPRTVLCLFELVALAAAHGYFAATGRPQAVLVHVDVG
                     TQNLGGMLHNAQRGRAAVLICAGRTPYTTDARQRGTRDVYIHWLQEQFDQHGIVRNFV
                     KWDYELRRGEQMGEVVARAFQIAASDPPGPVYLTLPREVLMTEVGTIAVPGRDQAPPA
                     KMGAGDPDALRGVASRLVRSERPLVLTATTGRTREGFEGLVRLAELLALPVVERRERT
                     NFPTTNPLHQGFQPGPLVESADVILVLDSDVPYIPTQTTPRADAAIVQIDLDPVKERI
                     PLWSFPLTTAVRADTSRALDLVADYATELITDGARRRIEVRRAELAARHRERRAAREA
                     AALAARGARPIAVEWLGYCLGQLQREAPECVFVDEALTSHVALWNHVDLEEPGTMFGS
                     GGSSLGWGLGAALGVKLARPDRQVVLA"
     gene            complement(1452..>2931)
                     /locus_tag="AC16CY_20140"
     CDS             complement(1452..>2931)
                     /locus_tag="AC16CY_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356173.1"
                     /translation="FVDLGFGLKRVASAGGPAVPITRLDGAGRGATWGPDETIVFATS
                     TNLTGLQMVSAAGGKPTVLTKPDAGRGEADHYWPEFLPGGQAVLFTVFSTTGPDNNQV
                     AVLDLRSGMHKILVRGTGAQYVPSGHLVYVAAGTLLAVPFDLDRLEVRGTPVPVEPAI
                     LTTSAGAAIFDVSGNGTLVYLSATRAIDARTLVWVDRGGREEILDDRPRAYIYPQLSP
                     DRTKVAIAILDQERDIWILDLRRQNLTRLTTHPGDDRYPVWAPDGQRLVWDSTRTGAY
                     NLYWQAADGSGDVERLTESPNTQASHTFTPDGKRLLLREDKPGTLQDLVVLELDGKRR
                     VTPLLNGPYNERNAEISPDGKWMAYESDESGQFEVYVRPFPDVNKSKSKISTGGGRQP
                     LWSADGRELFYRDLRGAVVGVTVKAVADTFEPGAPVTLVPPGYYSGSGGIFGRTYDVS
                     ADGKRFLMIKEANDSKNADAPTPTITVVQNWFEELKRLVPAN"
BASE COUNT          455 a          998 c          987 g          491 t
ORIGIN      
        1 cgcgagcacg acctggcgat ccgggcgcgc gagcttcacg ccgagcgccg cgcccagccc
       61 ccagccgagc gacgagccgc cgctgccgaa catcgtgccc ggctcctcga ggtcgacgtg
      121 gttccagagc gccacgtggc tggtgagcgc ctcgtccacg aacacgcact cgggcgcctc
      181 gcgctgaagc tggccgaggc agtacccgag ccactcgacg gcaatcggcc gcgccccgcg
      241 ggcagccagc gcggcagcct cgcgtgcggc ccggcgctcg cggtggcgtg cggccagttc
      301 agctcgacgg acctcgatgc gcctccgcgc gccgtcggtt atcagctccg tcgcgtagtc
      361 ggccacgaga tcgagcgcgc gcgacgtgtc ggcgcgaacg gccgtcgtca gcgggaagct
      421 ccagagcggt atccgctctt tcacgggatc cagatcgatc tgcacgatcg ccgcgtcggc
      481 gcggggcgtg gtctgggtgg ggatgtaggg cacgtcggag tcgagcacga ggatgacgtc
      541 ggccgactcg acgagcggtc cgggctggaa cccctggtgc agcggattgg tcgtcgggaa
      601 gttcgtgcgc tcccggcgct cgaccaccgg cagcgcgagc agctcggcga gcctgacgag
      661 cccctcgaag ccctcacggg tgcgtccggt cgttgccgtc agcaccagcg gacgctcgct
      721 ccggacgagg cgcgaggcga cgccgcgcag cgcgtccgga tctcccgcgc ccatcttcgc
      781 cggcggagcc tgatcgcgcc ccggcacggc gatggtgccc acctcggtca tgagcacctc
      841 gcgaggcagc gtgagataga cggggcccgg cggatcgctt gccgcgatct gaaacgcgcg
      901 agccaccacc tctcccatct gctctccgcg ccggagctcg taatcccact tcacgaagtt
      961 gcggacgatg ccgtgttgat cgaactgctc ctgcagccag tggatgtaga cgtcgcgcgt
     1021 gcctcgctgg cgcgcgtcgg tcgtatacgg tgtgcggccg gcgcagatca gcacggcggc
     1081 gcgtccccgc tgcgcgttgt gcagcatccc gccgaggttc tgcgtgccga cgtccacgtg
     1141 aacgagcacg gcttgcggcc gtccggtcgc cgcgaaatag ccgtgggccg cggccagcgc
     1201 gacgagctcg aacaggcaga gcacggtgcg cggcaccggc tgaccgagct gctccatctt
     1261 cgagagcgct tcctgaatgg agatcgtgtc ggtgcccggg ttgaggaaaa ggcactcgac
     1321 gtcctgcgcg accagcgcgc ggacgaaggc ctccgccgct tccgcaggtc gctgctcgac
     1381 gggctgttgg gcagttctgg cttcggcatc cggtcgtgat tgtgtcgcca tagtgaaagg
     1441 atatccgggc gtcagttcgc aggcacgagg cgcttcagtt cctcgaacca gttctgcacg
     1501 acggtgatgg tcggcgtagg cgcatcggcg ttcttcgagt cgttggcttc cttgatcatc
     1561 aggaagcgct tgccgtcggc cgacacatcg tacgtccggc cgaagatccc gccgctgccg
     1621 ctgtagtagc ccggcggcac caacgttacg ggggcgcccg gctcgaacgt gtctgcgacc
     1681 gccttcaccg tcacaccgac gacggccccg cggagatctc gatagaagag ctcgcgtccg
     1741 tctgctgacc acagcggctg cctcccgcca cccgtcgaga tcttggactt tgacttgttc
     1801 acgtccggaa atggccggac gtacacctcg aactgaccag actcgtcgga ctcgtaggcc
     1861 atccacttgc cgtcaggaga gatctcggcg ttgcgctcgt tgtatggacc gttgagcaag
     1921 ggcgtcacac gccgctttcc atcgagctcg aggacgacca gatcctggag cgttccgggc
     1981 ttgtcttcac gcaacaggag acgcttcccg tcgggcgtga acgtgtgcga cgcctgagtg
     2041 ttcgggctct ccgtcaaccg ctcgacgtcg cccgacccgt ccgctgcctg ccagtacagg
     2101 ttatatgcac ccgtgcgcgt cgagtcccat accagccgtt ggccgtccgg cgcccagacg
     2161 gggtagcggt cgtcgcccgg atgcgtcgtc agccgggtca gattctggcg ccgtagatcc
     2221 aggatccaga tgtcgcgctc ctggtcgaga atggcgatcg cgaccttcgt ccggtcgggt
     2281 gacagctgcg gatagatata cgcgcgcggc cggtcgtcca ggatctcctc gcgtcctcca
     2341 cgatcgaccc agaccagcgt gcgtgcgtcg atcgctcgag tcgcggacag atacacgagc
     2401 gtgccgttcc cagagacgtc gaaaatagcg gcgccagcgc tcgtcgtgag gattgccggc
     2461 tcgactggaa ccggggtccc tcggacctcc aaccggtcga gatcgaaggg gacggcaagc
     2521 agcgtgcccg cggcaacgta gaccagatgc ccgctcggta cgtattgcgc gccagtgccg
     2581 cggaccagaa tcttgtgcat gccgctccgc aggtcgagga ccgccacctg gttgttgtcg
     2641 ggtcccgtcg tggagaagac ggtgaagagc acggcctgtc cacccggtaa gaattccggc
     2701 cagtagtggt cagcttcgcc gcgcccggca tcgggcttgg tcagtacggt gggtttcccg
     2761 ccggctgccg acaccatttg cagtccggtc aggttcgtgc tggtcgcgaa gacaatggtc
     2821 tcgtcgggac cccacgtagc gcctcggccc gcgccgtcga ggcgggtaat cggcacggct
     2881 ggtccgccag cactcgcgac ccttttgagc ccgaagccaa ggtccacgaa a
//
LOCUS       DBNGSH010000878         4036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4694399, whole genome shotgun sequence.
ACCESSION   DBNGSH010000878 DBNGSH010000000
VERSION     DBNGSH010000878.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4694399"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2191)
                     /locus_tag="AC16CY_20145"
     CDS             complement(<1..2191)
                     /locus_tag="AC16CY_20145"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HIR3356174.1"
                     /translation="MADPGILDELPILRFLPEETRARVVSRFVPASFPFGGVLAEEGE
                     AADALYVLVSGRARVVKRTDHDDEVPLNVLHAGDSFGETELLHGWPRPTTVRATSDVL
                     ALRLDRAAFQELVAADPHIRTYLELQLKHDTLQRFFRDFPAFGRLPAEAMVGIVLAEL
                     EPRVAAAGEVIVAEGDPPGPLYLIEEGRVRVMKMVDGAPRYMASLVEGQYFGEVSALA
                     GKPRTATVEAMTTCRLLALSPETVTRLADGLPDFRRRLEERIQQYDFKNRAQTPAGIE
                     KELLPAGAAAQRQVDDAELDQAESEHAADEAPFEDAGHFVKRKRRIRRMRFVRQIDEM
                     DCGAACLAMIAGHFGRNVSLARIRQLVNTGLDGTSLRSLCSAAEELGLAARSVKASLH
                     NLDRMPLPAICHWDGEHWLVLYHVTPRSAFVADPALGYRKLPREEFERRWTAYAALFD
                     YTPAFEQAPRAARGLGWLWPIVKPHGPLIVKALGLAVVVSVLQMVLPVFTQVIVDRVL
                     VEQDLSLLRLLIVAMGGTMVFIVASVLAQRYLLSFVAVRIDASGLDFITRRLLALPMS
                     YFATRRTGDLQRRIEGMRQVRDLLVQHGISGVTAVAQLTVTAALMAVYSPWLTTVFLT
                     TAPFYALLMVMAVRWLRPTIYDLEDAYGRYHSYQVDAIKGIETVKAVGGEPMFRQLML
                     GQFQRVAHKLFRADFTVMTYDGAIDAVTFLGLGLFLWAGAHEVLGGRL"
     gene            complement(2198..3292)
                     /locus_tag="AC16CY_20150"
     CDS             complement(2198..3292)
                     /locus_tag="AC16CY_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356175.1"
                     /translation="MGISAASRSRLSAFWDQYSFSIGDVDFLWIDVAIGAMVHGEWSD
                     FERRLTEGLACSARAEAERTGLEERVVDEAATAFRYDRDLISGAEMNEWLDGVGVSGD
                     EWMAHLRRGVLRRMWTADMDDVVDRYAASPRQLEAAAVAEGICSGCFDAFERSFAGRV
                     AAIVAAGGAVPPAGSLVSPAIEASVNRLARQHAHWLAMRPEADTRERLAAALRLEESF
                     SSLSDRLTSKDCLREVVNAQALDWTIIEIDSITFATEHAAREAILCVREDGLSLHDVA
                     ALARRQVTRTRVFLEEIPQQHRDRLLSVELGQVLGPLAADDRFDVGAVIGRSAPSLDD
                     DRVAERARRAALDNATRRAAREHVTRRAKN"
     gene            3404..3550
                     /locus_tag="AC16CY_20155"
     CDS             3404..3550
                     /locus_tag="AC16CY_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356176.1"
                     /translation="MTAATLAFQKRILANQKRILANQKRIEANQAKLDRVLANQKAIL
                     ARLK"
     gene            complement(3543..>4036)
                     /locus_tag="AC16CY_20160"
     CDS             complement(3543..>4036)
                     /locus_tag="AC16CY_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356177.1"
                     /translation="PAEAHNGPPYPIVSNQAAGPYRISIWTDPDATDDGSLGGQFWVL
                     IDQPDAKALSPDLRARVSVRPLDRHGRLEAALAAPVDDNRARLFAALLMDHEGRFAVR
                     VEIESATGGGAIDSEVQATYDLRPPRAMLAIYAVPFALVGLLWAKRLARRRRAQAARR
                     PLT"
BASE COUNT          706 a         1390 c         1359 g          581 t
ORIGIN      
        1 tcagccgtcc gccgaggacc tcgtgcgcac ccgcccagag gaacaggcca agacccagga
       61 aggtcacggc gtcgatggcc ccgtcgtagg tcatgaccgt gaagtcggca cgaaacagct
      121 tgtgcgcaac gcgctggaac tggccgagca tcaactgcct gaacatcggc tctccgccca
      181 cggccttcac ggtctcgatc cccttgatgg cgtccacctg atacgagtgg taccggccgt
      241 aggcgtcttc gaggtcgtag atggtcggcc ggagccagcg gacggccatc accatgagga
      301 gcgcgtagaa cggagcagtg gtgaggaaca ctgtcgtcaa ccacgggctg tagacggcca
      361 tcagcgcggc ggtgacggtc aactgcgcca ctgccgtgac gccggaaata ccgtgttgga
      421 ccagcaggtc gcgaacctgc cgcatgccct cgatgcggcg ctgcaggtcg ccggtccgcc
      481 tcgtcgcgaa gtacgacatc ggcagcgcca gcaggcggcg tgtgatgaaa tccagtccgg
      541 acgcatcgat gcgcacggcg acaaaactga gcaggtaccg ctgcgccagc accgacgcga
      601 cgatgaacac catcgtcccg cccatcgcca cgatgaggag acgcagcagc gacaggtcct
      661 gctcgaccag cacgcgatcg acgatgacct gcgtgaagac cggcagcacc atctgcagca
      721 cgctgacgac cacggcgagg ccgagcgcct tgacgatgag cggcccgtgc ggcttgacga
      781 tcggccacag ccagccaagc ccgcgcgcgg cccgaggggc ctgctcgaag gcgggcgtgt
      841 aatcgaagag cgccgcgtac gcggtccatc ggcgctcgaa ttcctcgcga ggcagcttgc
      901 ggtacccgag cgcgggatcg gcgacgaatg cgctgcgcgg ggtgacgtga tagagcacga
      961 gccagtgctc tccgtcccag tgacagatgg ccggcagcgg catccgatcg aggttgtgca
     1021 gggaggcctt gaccgatcgc gcggccaggc ccagctcctc ggccgcggag cacaacgacc
     1081 gcaagctcgt gccgtcgagc cccgtgttga ccagctgccg gattcgcgcg agcgacacgt
     1141 tccgcccgaa gtggccggcg atcatggcca ggcacgcggc gccgcagtcc atttcgtcga
     1201 tctgccggac gaaccgcatg cgtcggatgc gtcgcttgcg cttgacgaaa tgcccggcgt
     1261 cctcgaacgg cgcctcgtcg gcggcgtgtt ccgactcggc ctgatcgagc tccgcgtcgt
     1321 caacctggcg ctgcgcggcc gcgcccgccg ggagcaactc cttctcgatg ccagccggcg
     1381 tctgcgcccg gttcttgaag tcgtactgct gaatccgctc ctcgagccgg cggcggaagt
     1441 ctggaagtcc gtccgccagc cgcgtcacgg tctccgggct gagcgcgagc agacggcacg
     1501 tcgtcatcgc ctcgaccgtc gcggttcgcg gcttcccggc cagcgctgac acctcaccga
     1561 agtactggcc ctcgacgagg ctcgccatgt agcgtggagc gccgtcgacc atcttcatca
     1621 cgcgcactct gccctcttcg atgaggtaca ggggccctgg aggatcgcct tccgcgacga
     1681 tgacttctcc cgcagctgcc acccgcggct cgagctcggc cagcacgatg cccaccatcg
     1741 cctcggccgg caggcggccg aatgccggaa agtcgcgaaa gaaccgctgc agcgtgtcgt
     1801 gtttgagctg gagctcgagg taggtgcgga tgtgcgggtc cgccgcgacc agttcctgaa
     1861 acgcggcgcg atcgagccgc agggccagca catcgctggt ggcccgtacc gtggtcggcc
     1921 ggggccagcc gtgcaacagc tcggtctcgc cgaagctgtc gcccgcatga agcacgttca
     1981 gcgggacctc gtcgtcgtgg tccgtgcgct tgacgacgcg agcgcggccg ctcacgagga
     2041 cgtaaagtgc gtcggccgcc tccccctcct cggccaggac accgccgaag gggaacgacg
     2101 ccgggacgaa gcggctgacg acccgcgcgc gagtctcctc tggaaggaac cggaggatag
     2161 gaagctcgtc gaggatcccg gggtccgcca caggacctca gttcttcgcc cgccgcgtca
     2221 cgtgctctcg agccgcgcgc cgcgtggcat tgtcgagcgc tgcgcggcgc gcacgctcgg
     2281 cgacgcggtc gtcgtcgaga gacggcgcgc tgcggccgat cacggcgccg acgtcgaatc
     2341 gatcgtcggc cgccaacggc cccaggacct gtcccagctc gaccgagagc agacgatccc
     2401 ggtgctgctg cggaatctct tcgaggaaga cgcgcgtccg cgtgacctga cgccgggcca
     2461 gcgcggcgac atcgtggagg gacaggccgt cttccctgac gcagaggatt gcttcgcgtg
     2521 cggcgtgctc ggtggcaaag gtgatcgagt cgatctcgat gatcgtccag tcgagcgcct
     2581 gggcgttgac gacctcgcga aggcagtctt tcgacgtcag tcgatcgctg agggacgaaa
     2641 agctctcctc gaggcgaaga gcggctgcaa gccgctcgcg cgtgtccgcc tcaggacgca
     2701 tcgccagcca gtgcgcgtgc tgtcgcgcga gcctgttgac agacgcctcg atcgccggcg
     2761 aaacgaggct gccggcaggc gggacggcgc cgcccgcggc gacgatcgcg gccacgcgtc
     2821 cggcaaacga ccgctcgaag gcgtcgaagc agccggagca gatgccctcg gccacggcgg
     2881 cggcctcgag ctggcgcggc gaggccgcat agcgatcgac cacgtcgtcc atgtcggcgg
     2941 tccacatgcg ccgcaggacg ccgcggcgca ggtgcgccat ccactcatcc ccggataccc
     3001 cgacgccgtc gagccactcg ttcatttcgg ccccggagat cagatcgcgg tcgtaccgga
     3061 acgccgtcgc cgcttcgtcc acgacgcgct cctccaaccc ggtgcgttcg gcctcggcgc
     3121 gcgccgagca cgccaatccc tcggtgagac gccgttcgaa gtcggaccac tcgccgtgca
     3181 ccatggcacc gatcgcgacg tcgatccaga ggaagtccac gtcgccgatc gagaaggagt
     3241 actgatccca gaacgccgag agccggctgc gtgacgcagc agaaatgccc atttccaagg
     3301 gggtggccgg caatcatagc agccagccgc acgaagatct tgctacagtc tggacatcgg
     3361 gccgacgcgc cattgcgtgt cgccatgtga aaaggagtcc ttgatgaccg ccgcgacgct
     3421 cgccttccag aagcggattc tggccaacca gaagcgcatt ctcgcgaacc agaaacggat
     3481 cgaagccaac caggccaagc tggacagggt cctcgccaac cagaaggcga tcctggcacg
     3541 gctcaagtaa gcgggcgtcg cgcggcctgg gcacgccgac gccgggcgag ccgcttcgcc
     3601 cacagcagcc ctaccagcgc gaagggtacg gcgtagatgg cgagcatggc ccgcggcgga
     3661 cgcaaatcgt aggtggcctg gacctcggag tcgatagcgc cgccgccggt tgcgctctcg
     3721 atctcgacgc gcacggcaaa gcggccttcg tgatccatca ggagcgccgc gaacagacgg
     3781 gcgcgattgt cgtcaaccgg tgcggccagg gccgcttcca ggcggccatg ccggtcgagc
     3841 ggacggaccg agacacgcgc gcgcagatcc gggctgagcg ccttcgcatc gggctgatcg
     3901 atcaacaccc agaactgccc tccgagcgaa ccatcgtcgg tggcatcggg atccgtccag
     3961 atggagatcc gatacgggcc ggcggcctgg ttcgagacga tcggatacgg cgggccgttg
     4021 tgagcctctg ccgggc
//
LOCUS       DBNGSH010000879         7847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2826184, whole genome shotgun sequence.
ACCESSION   DBNGSH010000879 DBNGSH010000000
VERSION     DBNGSH010000879.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2826184"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4092
                     /locus_tag="AC16CY_20165"
     CDS             <1..4092
                     /locus_tag="AC16CY_20165"
                     /inference="COORDINATES: protein motif:HMM:NF012873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIR3356178.1"
                     /translation="DELPYWLSTPRSTIGRLPIDEPQGTNSVGSARSVTVALTASETE
                     ALLRQVPDVYRTQINDVLLTAVADAFAAWTGERRLLIDLEGHGREELFADVDVSRTVG
                     WFTAMYPVALEWEPGNDPGASLKSIKEQLRKVPNRGIGYGLLRYLGGDAEGQLARLPR
                     AEMTFNYLGQVDQALPTSSRFVWAAESPGPAQSPRATRSHLLDVSGVISDGCLRLSLK
                     FSEHLHRRETIEQLAGRVVESLRSLIRHCQSAEAGGCTPSDFPMAELDQQALDRLVGA
                     AREIEDVYPLSPMQQGLLFHTLLDSASGVYVEQFCCTVHGKLDLSRFQSAWQHVLERQ
                     PVLRTSFRHEGLRQPLQIVHRRVPIEWVQQDWRELSTEEHRTELDAFLQEDRQRGFDL
                     SNPPLMRLAVLQIAADSYQIVWTYHHLLLDGWSVPIVLNEVLAAYGSFGGGGRVQPVV
                     SRPYRDLIAWHRRQDLEAASQFWRRTLKGFEAPTPLTVDSPHAQQAGEVSPHAEQGVE
                     LSKASTDALRSFGRQQQLTLNTLVQAAWGVLLSRYSGRDDVLFGATVSGRPASLPGVE
                     SMVGLFINTVPVRVRVLRSEPLVKWLQTVQSEQLEARDYEYCPLSRVQGWSELPAGTP
                     LFESLLVFENFPVDETLLKRQQELTIDGSRTIERTNYPLTLVAAPGQRFSLRINYDAS
                     RFDQVTVARMLGHLEALLEGMISDADRRIDDIPMLTGAERQDLLVAWNATEATYEGPR
                     TVHEMFEAQVGKVPDAVALVYKDQAVTYAGLDRWTNQLAQRLVALGVKPNALVGLFME
                     RGPEMVAGILGVLKAGGAYVPLPRDYPRQRLQFMLEDSAAALILTQEHLRDELAGGSA
                     AVECVDRHWRDEAAPAVRRCRTDVTADDLAYVMYTSGSTGRPKGTLIEHRSVANYLTW
                     CTRTYPLAEGCGAPVQSSIGFDATITSLIAPLLTGRRVVLLPESQEVEALGAALASDS
                     DFGLVKLTPAHLKMLGPMVPPDRAPGGTRAFVIGGEALTANDVAFWRTHAPRTKLVNE
                     YGPTETVVGCCVYEVSAATPTSGAVPIGRPIANTQLYVLDRGMAPMPMSVPGEIYIGG
                     AGLGRGYLNRPELTAEKFVPNPFSDEPGTRLYRTGDLGRHRLDGELEYHGRTDDQVKL
                     RGFRIELGEIEAVLSQHPQVREAVVLAREDGSRGKRLVAYVTSAEPSGVSVLELRSHL
                     QGALPSHMVPAAFVMLDALPLTANGKVDRRALPAPDQTRQELQVPFVAAATDAEQKIA
                     AIWKETIGIDTIGVDDNFFEAGGDSLIMIEVQWKLKDVFGRNVPMAQMFQHPTVRSLA
                     AALSRRSPEEDEAASGASRSRAQARLAAVAERGRRRDTRRGRS"
     gene            4184..5476
                     /locus_tag="AC16CY_20170"
     CDS             4184..5476
                     /locus_tag="AC16CY_20170"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR3356179.1"
                     /translation="MVTRLLTEAERPYPAGEAGTPLVIQATSSSDLSFLKAFLSAHTQ
                     SIRNDLYGHGAVLLRGFRITSPRDFEEALTSIQGFRPMKGYFMSEQGRDPVKGTDYVF
                     DTSTFFISGGDFRFGGVHSENYYSPDVPQFQCLCCLKRPWIGGETALFHMANTYEGLR
                     ERLAPKLETESFVANVISLQSVADRYKLPEAEVETFLKDNGVELIQWNGRKVMLNHKP
                     SILCHPHTGRPSLQVNLSLELKNLDHQRHFLAGYGGMKWALHRLAWRRPRVLKALTYI
                     AHLSRAIRHPSLFWHFLLEPLGRRFVRSAPEQQPAKPAGAPAIPRIGQRLDPEDQRLL
                     GRALWEHSSVFTWQAGDVLIVDNLQMHHAGMPGFGPRDLKVVMGNPISVSTRGSTGLH
                     RLGVDDAFVSLHDRLAQLAARHAGLPPVATEHRGVSSR"
     gene            5509..6357
                     /locus_tag="AC16CY_20175"
     CDS             5509..6357
                     /locus_tag="AC16CY_20175"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR3356180.1"
                     /translation="MAKLAELVARRSTSFARTLPTVFILGATKSASTSLTNMLWHHPA
                     HVSAFAKELMYLQQLPNFENQWEFRRSVAFVWGRISNGHARYSLAGYRKFFPLKLHML
                     LRAISQGQAVTSECDPFNLYCPTAQRRIEAFAKDPRFIISMRNPIDRAYSDYNMHATR
                     MDERRSFEQCIEDELSGRETQFRKQFLNQSVYEPHVRRWIEAFGRERFLILKAEDLFA
                     DPERAAAEMFRFLNLPDAPTDLTPTNTGRYAAKLSTDTRARLAEYFAPHNERLFDLVG
                     WKHDLD"
     gene            6416..>7847
                     /locus_tag="AC16CY_20180"
     CDS             6416..>7847
                     /locus_tag="AC16CY_20180"
                     /EC_number="2.3.1.-"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0034081 - polyketide synthase complex
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HIR3356181.1"
                     /translation="MAGRFPGAANAAEFWRNLANGVESISFFSKEELLSEGVSGDLIA
                     DPAYVGARGVIRDAEWFDAGFFGFAPRQAEVMDPQHRLFMECVWEVLESGGYDPQRYP
                     GAIGVYAGASMSSYLLENLISNRNARGSSGGFETVIANDKDFLATGVSYRLNLKGPSI
                     TVQSSCSTSLVAVHVACQSLLNGECDMALAGGVSIVFPQKSGYVYREGGTRSPDGHCR
                     AFDAKAGGMVSGSGVGVVLLKRLDRALEDGDHIRAVIRGSAVNNDGNLKVGYTAPSVE
                     GQSAVIREAQAVAGVDPATITYVEAHGTGTRLGDPCEISALTQAFHAGTDRKRFCAIG
                     SVKTNIGHLDVAAGVAGLIKTVLALENRRIPASLHFERPNPEIDFDSTPFFVNDTLAE
                     WPEGPTPRRAGVSSFGIGGTNAHVVLEEAPQLESAPDSRARHLVVLSAKSAAALARAT
                     SNLADHLRASSETSLADVAHTLQVGRS"
BASE COUNT         1400 a         2505 c         2589 g         1353 t
ORIGIN      
        1 gacgagctgc cctactggct gtccacgccg cgcagcacga ttggccggct tcccatcgac
       61 gaaccgcagg gcacaaattc cgttggctcg gcgcggagcg tgaccgtggc gctgaccgcg
      121 tctgagaccg aggcgctgct gcggcaggtt cctgacgtct accggacgca gatcaacgac
      181 gtcctgctga ctgccgtggc cgacgcgttc gccgcctgga ctggagagcg ccggctgctg
      241 atcgacctcg agggtcacgg tcgcgaagag ctgttcgcgg acgtggacgt gtcgcgcacc
      301 gtgggctggt tcacggcgat gtatccggtg gcgctcgaat gggagccagg caacgatccg
      361 ggagcatcgc tgaagtcaat caaggagcag cttcgcaagg tgccgaaccg cggcatcggc
      421 tacggtctgt tgcgatacct ggggggcgat gcagaggggc agctcgcccg cctgccccgg
      481 gctgaaatga cgttcaacta cctcgggcaa gtcgatcagg ctctgcccac gtcgtcgcgg
      541 ttcgtctggg cggccgaatc accgggaccg gcccagagcc cgcgtgccac tcggagccac
      601 ctgctggacg tgagcggcgt aatcagcgat ggctgcctgc ggctgtccct gaagttcagt
      661 gaacacctgc accgccgcga gacgatcgag caactggccg gacgcgtcgt cgagtcgctt
      721 cgatccctga tacggcactg tcagtccgcc gaggccgggg gctgcacgcc ttcagacttc
      781 ccgatggcgg agctggacca gcaggcgctg gatcggctcg tgggagccgc ccgtgagatc
      841 gaggacgtgt atcccctctc gccgatgcag cagggcctgc tgttccacac gctgctggac
      901 agcgcgtcgg gtgtctacgt cgagcaattc tgctgcacgg tacacggcaa gctcgatctg
      961 tccaggttcc agtcggcctg gcagcacgtg ctcgaacggc aacccgtgtt gcgcacctcg
     1021 tttcgccatg agggactgcg gcagcccttg cagatcgttc accgccgggt ccccatcgaa
     1081 tgggtgcagc aggattggcg ggagctgtca accgaggagc accgcacgga gctcgacgcg
     1141 ttcctgcagg aagatcgtca gcgcgggttc gacctgtcga atcctccctt gatgcggctc
     1201 gccgtactgc agatcgcggc ggactcctac cagattgtct ggacctatca ccatcttctg
     1261 ctcgacggct ggtcggtgcc catcgttctc aacgaagtgc tggcggctta cgggtcgttc
     1321 ggcggcggcg gacgagtaca gccggtcgta tcccgcccgt accgagacct cattgcctgg
     1381 caccgtcgcc aggatctcga ggccgcatcg cagttctggc ggcgcacgtt gaagggattc
     1441 gaggccccga caccgctgac cgtcgattcc ccgcacgccc agcaggctgg cgaagtatca
     1501 ccgcacgcgg aacaaggggt cgagctctcg aaggcatcca ccgacgcgct ccggtcattc
     1561 gggcggcagc aacaactgac gctgaacacc ctggttcaag cggcatgggg ggtgttgttg
     1621 agccgctaca gcggtcgcga cgacgtgctc ttcggagcga ccgtctccgg acgtcccgct
     1681 tcgctgcccg gcgtggagtc gatggtgggg ctcttcatca ataccgtgcc ggtgcgcgtg
     1741 cgggtgctga ggagcgagcc gctcgtgaag tggctgcaga ccgtgcagag cgaacagctc
     1801 gaggcacgcg actacgagta ctgcccgctc agcagagttc aggggtggag cgagcttccg
     1861 gcagggacgc cgttgttcga gagcctgctc gtcttcgaaa acttcccggt tgacgagacg
     1921 ctgctcaaga gacagcagga gctgacaatc gatgggtcgc gcactatcga gcgcacgaac
     1981 tatccattga ccctggtggc ggcgccaggc cagcggttct cgcttcgcat caactacgac
     2041 gccagccgtt tcgatcaggt gacggtcgca cgaatgctcg gccacctcga ggcgttgctc
     2101 gagggcatga tcagcgacgc cgatcgacgc atcgacgaca tcccgatgtt gaccggggcg
     2161 gagcggcagg atctgctggt cgcctggaac gccacggagg ccacgtacga aggtccgcgc
     2221 accgtgcacg agatgttcga ggcgcaggtt gggaaggtgc cggacgcggt tgcgctggtc
     2281 tacaaagacc aggcggtgac gtatgcgggg ctcgaccgct ggaccaatca gctggcccag
     2341 cgcctggtgg cattgggagt gaagccgaac gcgctggtgg gcctgttcat ggaacggggt
     2401 cccgagatgg tggccggcat ccttggagtc ctcaaggccg ggggcgcgta cgtgccgctg
     2461 ccgcgagact atccgagaca gcggcttcag ttcatgctcg aagacagcgc ggcggccttg
     2521 atcctgacgc aggaacacct gcgggacgaa ctcgccggcg gttcggcggc ggtggaatgc
     2581 gtcgatcgtc actggcggga cgaagcggcc ccagccgtga ggcgttgccg gaccgacgtc
     2641 acggccgacg acctcgcata cgtgatgtac acctcgggat ccaccgggcg acccaagggc
     2701 accctgatcg aacaccggag cgtggccaat taccttacgt ggtgcacgcg gacctaccca
     2761 ttggcggagg gctgtggagc cccggtgcaa tcgtcgattg gattcgatgc gaccatcacg
     2821 agcctgattg ccccgctcct caccggccgc cgcgtggtgc tgctcccgga gtcgcaggaa
     2881 gtcgaagcac ttggtgcggc cctggcctcg gacagcgact tcggcctggt gaagctcacg
     2941 ccggcccacc tgaagatgct cggcccgatg gtcccgccgg acagggcccc gggaggcacg
     3001 cgagcgttcg tcatcggcgg cgaggcgctc acggcaaacg acgtcgcgtt ctggcggaca
     3061 cacgccccgc ggacgaagct cgtcaacgaa tacggcccga ccgagaccgt cgtcgggtgc
     3121 tgtgtctacg aagtctccgc ggcgacgccg acctccggtg cggtgccgat cggccggccg
     3181 atcgccaaca cgcagctcta cgtactcgat cgcggcatgg ccccaatgcc gatgagcgtg
     3241 ccaggtgaaa tctacattgg cggcgccggc ctgggtcggg gctacctgaa tcggccggag
     3301 ctgaccgccg agaaattcgt ccccaatccg ttcagcgatg agccggggac ccggctctac
     3361 cggacgggcg atctcggccg ccatcgcctc gacggcgagc tcgagtatca cgggcgcacg
     3421 gacgatcagg taaagcttcg ggggttccgc atcgagctcg gcgaaatcga agccgtgctg
     3481 agtcagcacc cgcaggtgcg cgaagcggtc gtgctggctc gcgaggacgg ctcgcgtggc
     3541 aagcggctcg tggcctatgt gaccagcgcc gaaccgtcgg gggtatcggt cctcgagctg
     3601 cgaagccatc tccagggcgc cctgccgagc cacatggtac ctgcggcctt cgtcatgctc
     3661 gacgccctgc cgctcactgc caacggcaag gtcgatcgcc gcgcgctgcc ggcgcccgat
     3721 cagacccgtc aggagctgca ggtcccgttc gtcgccgccg ccaccgatgc cgagcagaag
     3781 atcgccgcga tctggaagga gaccatcggc atcgacacca tcggcgtcga tgacaacttc
     3841 ttcgaggcgg gcggagactc gctcatcatg atcgaagtgc agtggaagct gaaggacgtc
     3901 ttcggccgga acgtgccgat ggcccagatg ttccaacacc ccaccgtgcg gtccctggcg
     3961 gcagcgttga gtcggcggtc ccccgaagaa gatgaggccg ccagcggcgc gagccggagt
     4021 cgagcgcagg cacggctggc ggcggtcgca gagcgggggc ggcgccgaga cactcgccgc
     4081 ggcagatcgt gacgcccacg tcccgccgcg ccactcgtaa ccgacacacg tgaagggaag
     4141 tatcgcagtg aagaacggtc caagcgtgtc acagtatgaa caggtggtca ctcgccttct
     4201 cacggaggcc gagcgtccct atcccgcggg tgaggctggt actcccctcg tcattcaggc
     4261 gacctcctcc tcggacctgt cgttcctgaa ggcgtttctt tcggctcaca cgcagagtat
     4321 ccgaaacgat ctgtacggcc atggagcggt tctcttgcgg ggcttccgga tcacctcgcc
     4381 gcgggatttc gaggaggccc tgacgtcgat acagggattc cggccgatga agggctactt
     4441 catgtccgaa caaggacgcg atcctgtcaa gggaacggac tatgtcttcg atacgagcac
     4501 gttcttcatc agcgggggcg atttccgatt cggcggagtt cattcagaga actactactc
     4561 cccggatgtc cctcagttcc aatgcctgtg ctgtctgaag agaccctgga tcggcgggga
     4621 gacggccctc tttcacatgg ccaacacata cgaagggttg cgggagcgcc tggcgccgaa
     4681 actcgagacg gagtcgttcg tcgcgaacgt catctcctta cagtcggtgg ccgaccggta
     4741 caaactgcca gaggccgagg tcgagacgtt cctgaaggac aacggcgttg agttgatcca
     4801 gtggaatgga agaaaggtga tgctcaacca caagcccagt attctgtgcc accctcacac
     4861 cggacgcccg tcgcttcagg tcaacctgtc gctggagttg aagaacctgg accaccagcg
     4921 ccactttctg gcgggctacg gcggcatgaa atgggccctt caccggctcg cgtggagacg
     4981 accgcgggtc ctgaaggcgc tgacctacat cgctcacctc tcccgggcga ttcggcaccc
     5041 cagtctgttc tggcacttcc tcctggagcc gctcgggcgc cggtttgtca ggtcagctcc
     5101 ggaacagcag cctgcgaagc cggcgggcgc gccggcgatt ccgcgcattg gccagaggtt
     5161 ggatccggag gaccagcggc tgctcgggcg tgcgctgtgg gagcattcgt cggtgttcac
     5221 ctggcaggcc ggcgacgtgc tgattgtcga caacctgcag atgcatcacg cggggatgcc
     5281 agggttcggg ccgagggatc tcaaggtcgt catgggcaat ccgatttcgg tttcgacccg
     5341 tggttcgacc ggcctgcatc ggctgggagt cgacgacgcc ttcgtctcgc tgcatgaccg
     5401 gctcgcgcaa ctcgcggccc gccacgccgg gctgccacca gtcgcgaccg agcatcgcgg
     5461 agtgtcgtca cgttgaaggc gcggcgaatc cggcgtcacg cgtgggtcat ggcgaagctg
     5521 gccgagctcg tggccagacg cagcacctcc ttcgcccgca cgttgcccac agtcttcatt
     5581 ctgggtgcca ccaagtccgc gtccacgtcg ttgacgaaca tgctgtggca tcaccccgcg
     5641 cacgtcagcg ccttcgcgaa ggagctgatg tacttgcagc aactcccgaa cttcgagaat
     5701 caatgggagt ttcgccggtc cgtcgcgttc gtctgggggc ggatcagcaa tggtcacgcc
     5761 cgatacagcc tggccggcta tcggaagttc ttcccgttga agctgcacat gctgcttcgg
     5821 gcgatctccc agggtcaggc cgtgaccagc gagtgcgatc ccttcaacct gtattgtcca
     5881 accgcccaga ggcgtatcga ggccttcgcg aaggatcccc gtttcattat cagcatgcgt
     5941 aacccgattg accgcgccta ctcggactac aacatgcatg cgacgaggat ggatgagagg
     6001 cgcagcttcg aacagtgcat agaggatgag ctgagcgggc gcgagacgca gttccgcaaa
     6061 caattcctga accagagcgt ctacgagccg cacgtgcggc gctggatcga ggcgttcggg
     6121 agggagcgct tcctgattct gaaagcggaa gacctcttcg ccgatccgga gcgagccgca
     6181 gcagagatgt tccgattcct gaacctgcca gacgcgccga cggatctcac gccgacgaat
     6241 acgggccgct acgcggcgaa gctgagcacg gacactcgtg ccaggctcgc ggagtatttt
     6301 gctccccaca acgagaggct gttcgatcta gttggctgga agcacgacct ggattgaccc
     6361 cgacagggcc gcgaccgtga cagacacaca gggaacggag atcgccgtca tcggcatggc
     6421 cggccgcttt ccaggtgcgg ccaacgcggc ggagttctgg cgcaacctcg ccaacggggt
     6481 ggagtccatc tcgttcttct cgaaagagga gctgctctct gaaggcgtca gcggcgatct
     6541 gatcgccgac cccgcatacg tcggggcaag gggagtcatt cgcgacgccg agtggttcga
     6601 cgccgggttc ttcggcttcg ccccccggca agccgaggtc atggacccac agcaccgcct
     6661 cttcatggag tgtgtctggg aggttctcga gtccggcggc tacgatcctc aacggtatcc
     6721 cggcgcgatc ggcgtctacg ccggagcgtc aatgagcagt tacctcctcg agaacctgat
     6781 ctccaaccgc aacgcgcgcg gttcatccgg tggcttcgag acggtcattg cgaacgacaa
     6841 ggattttctg gcgacggggg tctcctacag actgaatctg aagggcccca gcatcacggt
     6901 tcagtcctcc tgctccacgt ctctggtggc cgtccatgtc gcctgccaaa gccttctcaa
     6961 cggtgagtgc gatatggcgc tggccggcgg cgtctcgatc gtcttcccac agaagtcggg
     7021 atacgtgtac cgcgaaggag gcacccgttc acccgacggt cattgccggg cattcgacgc
     7081 gaaggccgga ggaatggtga gcggcagcgg ggtcggcgtc gtacttctga agagactcga
     7141 ccgggcgctc gaggacgggg accacatccg ggccgtcatt cgcggctcgg ccgtcaacaa
     7201 cgacgggaac ctcaaggtcg gctataccgc gccgagcgtg gaggggcagt ccgccgtcat
     7261 tcgcgaggcg caggccgtcg ccggagtgga tccggccacg attacctacg tcgaggccca
     7321 cggaacgggg accagactgg gcgatccgtg tgagatcagc gcattgacac aggcgtttca
     7381 cgccgggacg gacaggaagc ggttctgtgc gatcgggtcg gtgaaaacca acatcgggca
     7441 cctcgacgtc gccgcaggcg tcgcgggtct gatcaagacg gtactcgcac tcgagaatcg
     7501 tcgcatcccg gcgagcctgc acttcgagcg gccgaatccg gagattgatt tcgactccac
     7561 tccgttcttc gtcaacgaca cgctcgccga atggcccgag gggccaacgc cccgacgggc
     7621 gggggtcagt tcgttcggca tcggcggaac gaacgcccat gtcgtgctgg aagaagcgcc
     7681 gcagctcgag tcggctcccg attcccgtgc gcgccacctc gttgtgttat cggcgaagag
     7741 cgcggcggca ctggctcgag cgacgagcaa tcttgccgat cacctgcgag cgagctccga
     7801 gacatccctc gccgatgtcg cgcatacgct gcaggtcggg cgcagcg
//
LOCUS       DBNGSH010000880         3130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4353993, whole genome shotgun sequence.
ACCESSION   DBNGSH010000880 DBNGSH010000000
VERSION     DBNGSH010000880.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4353993"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..901
                     /locus_tag="AC16CY_20185"
     CDS             266..901
                     /locus_tag="AC16CY_20185"
                     /inference="COORDINATES: protein motif:HMM:NF024934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3356182.1"
                     /translation="MPFIRMSTVTTGETLQAHRRRIKDVYFPNGGVFSITNEMQNGGL
                     VEVATVGREGMLGIGVFFGDLAGIGRTFRQVANGRLPSMAASRFVKETAGAGPFRDVV
                     RVYAQANLLQIMQCTACNALHSVTERCCRWLLQTHDRVDDDTFSLKQEFLAVMLGVRR
                     PTVTVVLQTLQREGLIASRYGRIRILERKRLEAASCECYRVIRAHFARLGL"
     gene            950..1084
                     /locus_tag="AC16CY_20190"
     CDS             950..1084
                     /locus_tag="AC16CY_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356183.1"
                     /translation="MNDSWFGRAREVACDLLIATALIWTLPLLLGAAVALVQLLLGSD
                     "
     gene            complement(1415..2092)
                     /locus_tag="AC16CY_20195"
     CDS             complement(1415..2092)
                     /locus_tag="AC16CY_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3356184.1"
                     /translation="MSKAKARPVKGSQAPTFDVEAFLESVGAARRLVVFPKGKVVFAQ
                     GEPSDAVMYIQKGGVRISVLSRSGKEAVVAMLGPGDFFGEGALTGQPVRIGTATATTH
                     VTVFVIEKGAMLRLLHEEPAFSDAFISFMLSRNIRIEADLVDQLFNSSEKRLARALLL
                     LAQYTGQTGAERTIPRISQETLADMIGTTRSRVNFFMNKFRELGFIEYNGGLKINSAL
                     LSVVLHD"
     gene            complement(2529..2729)
                     /locus_tag="AC16CY_20200"
     CDS             complement(2529..2729)
                     /locus_tag="AC16CY_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356185.1"
                     /translation="MELQRLKMTVSAAWVVTVFAVAMAVGVSGVAMAAAAAFGLLPPL
                     ALLLFWNDPAQTMSQIINKARR"
     gene            2885..>3130
                     /locus_tag="AC16CY_20205"
     CDS             2885..>3130
                     /locus_tag="AC16CY_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR3356186.1"
                     /translation="MAQPGSVTRREFVKTAGTALAGTSLMNAGPSAGGRPGPPVRRYA
                     IIGTGDRATSMWGRPVLQEYSDVVELVGLCDVNPIRAE"
BASE COUNT          541 a         1038 c          986 g          565 t
ORIGIN      
        1 acacgcgcac cgcgcctgcg agctggccgc gagttttgac ttgaatgacg cgtgggaatt
       61 cgacgccgaa cggttccgtt cggccgtgga atcagtcgct cgctagacgg acacgcaaag
      121 gctcgagtac ctgacctgtt cagggttgct cacaggagag gcgatgccat ccgcgccatt
      181 acgttcgacc cgtcttttgc ctgacgggac ccctttgcac aatcggctcc ttgcggctct
      241 gccgcgtgcc gaatacgacc ggatcgtgcc cttcatccga atgagcaccg tgaccaccgg
      301 cgaaaccctg caggcgcacc gtaggcggat caaggacgtc tacttcccga acggcggcgt
      361 cttctccatc accaacgaaa tgcagaacgg cgggctggtg gaagtggcaa ccgtggggcg
      421 cgagggcatg ctggggatcg gcgtcttctt cggcgacctg gccggaatcg gccgcacgtt
      481 ccggcaggtg gccaatggcc ggctgccgtc gatggccgcc tcgagattcg tcaaggagac
      541 cgccggcgcc ggaccatttc gcgatgtcgt ccgcgtctac gctcaggcga acctgctgca
      601 gatcatgcag tgcaccgcgt gcaacgcgct acacagcgtg accgagcggt gctgccggtg
      661 gctgctgcag acgcacgatc gcgttgacga cgacaccttc tcgctcaaac aggaattcct
      721 tgcagtcatg ctcggcgtcc gtcgcccgac ggtcacggtg gtgctgcaga ccctgcagcg
      781 cgaggggttg atcgcgagcc gctatgggcg aatccgcatc ctcgaacgca agcgcctgga
      841 agccgcctcg tgcgagtgct accgcgtcat tcgcgcccat ttcgcacggc tcgggttgta
      901 gcgcacggca ccacgcccag gaccgatgtc tgcgttaccc agtcccaccg tgaacgactc
      961 ctggttcggg cgtgcccggg aagtggcatg cgatctcctg atcgccaccg ctctcatttg
     1021 gacgctgccg ctgctgctcg gcgccgcggt cgccctcgta cagctgctct tgggctcgga
     1081 ttagcgtgtc cacgcagagc gaccgcgcag tgttccaaac agcacagttc caaacaggac
     1141 atccgcaggc atcccatcct gtcaacatgc ggtccgtagc caacgaggga tgcgatgccg
     1201 aaatccggag tctcacgtcg cgcacgccgg gaccaggaac cacccgatcg agcggagtgg
     1261 gccggcgcag tggaaggcca ggtcaaggag atctatcacg agatcggcgc acaggtgaaa
     1321 cgaatgcagc gactgcagga gcaagcggac gagttgcgga cgacgcttct cctctgggtc
     1381 gttcccccag ccgggaacgc caggcgcccg cccgctagtc gtggagaacg acgctcagga
     1441 gagccgagtt gatcttgagg ccgccgttgt attcgatgaa ccccagttcc cggaacttgt
     1501 tcatgaaaaa gttcacgcgc gatcgtgtcg tcccgatcat gtccgccagt gtttcctggg
     1561 agatcctcgg tatcgttcgc tccgcgccgg tctgaccggt gtactgtgcc agcagcagaa
     1621 gcgcccgcgc gagccgcttc tcgctggagt tgaacagctg gtcgacgagg tccgcttcga
     1681 tgcggatgtt cctcgacagc atgaacgaga tgaacgcgtc ggagaacgcc ggctcctcgt
     1741 ggagcagccg gagcatcgcg cccttctcga tcacgaagac cgtgacgtgc gtcgtcgccg
     1801 tcgcggtccc gattcgaacc ggctggccag tcagcgcgcc ttctccgaag aagtccccgg
     1861 ggcccagcat ggccacgacc gcttcttttc cgctccggga caggaccgag atcctgacgc
     1921 cgcccttctg gatgtacatc accgcgtcgc tcggttcgcc ctgcgcgaag acgactttcc
     1981 ccttcgggaa caccaccagg cggcgcgcgg cacccacgga ttcgaggaac gcttcgacgt
     2041 cgaacgtcgg tgcctggctg cccttcacgg gccgtgcctt cgcctttgac atcacaattc
     2101 ggagaaatta tcgcacgcga ctccgctctc cggcgtcatc gcgtgggtgt ccactgtgga
     2161 acagcgcgcc tctgccgctt ttggtaggct caatgcgcat ggtgttgttt ccgcgtcccg
     2221 cgccccggtc tcatgtcgag tgtccgcagt gcggatcgcc acacgccttc gtcacgctcg
     2281 cgcgcgccga cacgcagtgc tctttctgtc cccagtgcca gtatctctgg gacacgcttc
     2341 gtgcctctca cccggcgtac tcgtctggtc gtcccgcgac tgttcgcgac tgagcaacgc
     2401 agggttgcgc gaagccgatg tgggttatgt gacgttcctg agtggatacg cgcgtgaggc
     2461 caacggtgac cgcatcgacc tgtattgctg agctgccgac acgagcggcg cgctggcgcg
     2521 cgggagggtt atcgccgcgc cttgttgatg atctggctca tcgtctgcgc cggatcgttc
     2581 cagaagagca ggagcgcgag cggcggcagc aacccgaagg cggccgcggc tgccatggcg
     2641 acacccgaga caccgaccgc catcgccacg gcgaacacag tgacgaccca cgcggcggag
     2701 acggtcatct tgaggcgttg cagttccatg gcgacagcat actggagcag cggctgtcgc
     2761 actgttcaaa acggcacgta cccgccccgc agcctgctgc cgatcatgaa cagcgatcgt
     2821 gcgcatcctg gcacactcgg ggcgcagcgg ctattatccg gcctcagatc caaggagcat
     2881 cgacgtggca cagcccggca gcgtgacgcg gcgcgagttc gtgaagacgg cgggcaccgc
     2941 actcgcgggc acgtcgttga tgaatgccgg accgtccgcc ggcggccgcc ccggcccgcc
     3001 tgtccggcga tacgccatca tcggcaccgg cgatcgcgcg acgtccatgt ggggacgccc
     3061 ggttcttcag gagtattccg acgtcgtcga gcttgtcgga ttgtgcgatg tcaatccgat
     3121 acgcgccgag
//
LOCUS       DBNGSH010000881        16998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2826422, whole genome shotgun sequence.
ACCESSION   DBNGSH010000881 DBNGSH010000000
VERSION     DBNGSH010000881.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2826422"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(430..1626)
                     /locus_tag="AC16CY_20210"
     CDS             complement(430..1626)
                     /locus_tag="AC16CY_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356187.1"
                     /translation="MITLTWGIVGALLLSVAVELAARFWIRRKGDYFVFPPGLRLRLK
                     IDRDLFPQLERETRFEVNRDGERGGEVPRSVDRLHRILVAGGSQPEGFLLDQYTAWPG
                     ALQRLLERPEALRRLGATHVHVGSIARSGVGSEALNLILERVLPRYPRLSTIIVLVGA
                     SDVLRWLEDGAPPAPPRRARVADVFRCHPEGPFGWTPRALAAFELLRRARRRWLRPVE
                     VHERAGKADAQARAMRARAKEIRDTMPDPSSMLDHFELHLRALLRKAQAHADRVLVVR
                     QPWLDKVFTPEEVACMWHGGAGRVWREDVTTYYSFDVCSRLMALLDARAVRVAKELHL
                     EHVDLMPLLEPSLEDYYDGFHATPSGSKTVARAVAAAVLRQPMTRPQLTPQRLPERAA
                     MYAQTM"
     gene            complement(1640..2764)
                     /locus_tag="AC16CY_20215"
     CDS             complement(1640..2764)
                     /locus_tag="AC16CY_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356188.1"
                     /translation="MSAALAQVRRVNAHRTAGVRRFEEADIPQVAALHQRCWPDGVGV
                     QRSYRGYFTEVFLENPAQRGPARSLVYHESDGRIVGFLGIVPRRMAMGGRHVEAAVSS
                     QFIVDPASAVGLVALKLSKTFLEGPQDVSIADEANDVSRIIWEGLGGTTARLLSLYWT
                     RPLRPAGLALSYLRGRRPFAPLATVAQPFARLADACAARLPGSHFRQSRPAVPPCDLY
                     VHEVLASVPQLGEAALRVEHDERNFQWLLDRAAGRRGARRLLTGAVKDGRTMAGWYIA
                     VLDRDGVAEVAQVAATPSSIHDVLDHLFHHAWREGALAATGRMEPRFMQALSDRYCLF
                     HRRGPWVLVKARYPELVRPFETGDAWFSPFDGEWSLRFQG"
     gene            complement(2761..3963)
                     /locus_tag="AC16CY_20220"
     CDS             complement(2761..3963)
                     /locus_tag="AC16CY_20220"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3356189.1"
                     /translation="MRGNPAAVDLPAPPRHARASGRAPLAIELKRDLELAPEDDVTLG
                     ALIDSRPHVGVFLSRAWLSGFFAEPPPGSEPFLLMLREGTTLRGIVPIAVRRRNTLVH
                     VSLLGGGLGSDRIDLLAERGFEPACADRFLAWLGESFGRRGFVLELRDAADSSALWGA
                     VHRTAQEGTLRLVLEPREVYTLPHLDIIDLKSLYDGRLASESLARTLGKHRRWLEGRG
                     RLTIEVLRERGDVISAFESLVGFLRARWQGATHRSALDDPRAQRFHRHVLPLLLGEGR
                     LRMLRVTSDLRTVAVFYGIASARWWGYYLAGYDREWAGRIHLGQLTLAAAIDAAAREG
                     AMEFDFLKGVERVKYLWPVRDRITMDADVYSAQPASQFARAGHATRTVAAALVKSARG
                     LFLTTGHR"
     gene            complement(3960..5468)
                     /locus_tag="AC16CY_20225"
     CDS             complement(3960..5468)
                     /locus_tag="AC16CY_20225"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR3356190.1"
                     /translation="MLRNVGSNWALILVTIAASYLLTPFIIHALGEEGYGTWTLITTL
                     TGHISLLALGVPMACVRYLSQHVAAGDTRRMNQTIGSCVGLYGVVGVVAAVLGALLLL
                     FFQQYSIPDGWKAESLLAFWVMVVTVSASFMGFLPEGILFAHHDFVRRNVIRMAGVLL
                     RTGLTIGLLALDASLVALASIQLTCLAFDFFVSWLIISRRYPEVRIGLAGFNLGVVRE
                     IFSFSVWVLLLTAGARLSFESDALVIGAFMGVGAIPFYVVANSLIVYLIDFMTSIAAV
                     VSPMTTKLNTEGRIDEVRAMFLKWSKVALSLTIMAGLFLCVLGPRFIAWWIDPSYEQP
                     AGGVLQILMVSSFIFLPVRGVAVPILMGLGKPRMPALAFIAAGLLNVGLSMALIGPLG
                     LAGVALGTAIPNVLFSLVVLTVVCRELDVSVAHWTRYVVPRATLGALPVLALLLWFRL
                     GLQVQSLTGLLVAGIVMVLLFGLVWIGFVYRGDPYVDVRAQKLLVMRAWGRA"
     gene            complement(5509..6315)
                     /locus_tag="AC16CY_20230"
     CDS             complement(5509..6315)
                     /locus_tag="AC16CY_20230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3356191.1"
                     /translation="MRRLVKAALGQTLFTSRLHQLLLKDAAVVVAFHRVFPVGARRER
                     PSGLTIDLDLFERYCRFFQRYFHVIPLQELVTRLERNQDLNRRLVITFDDGYRDNFEH
                     AAPILERCSLPATFFVVTQWMGTDVVPWWDRRHGRRYPWMTWDEVRSLHRRGFDIGAH
                     TRTHADLGLVRDVTARREIQGARIELEERLGAPVASFAYPYGGRHNLTEANRELVKDA
                     GYRCCCSVFGGINGPGRSPFDVRRVAMSPWCTTPQQFGFEVLLGRTLQAA"
     gene            complement(6312..8063)
                     /locus_tag="AC16CY_20235"
     CDS             complement(6312..8063)
                     /locus_tag="AC16CY_20235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3356192.1"
                     /translation="MTEGRKLRVCHVMSADLWAGAEVQVASTVAYLVGDPGISLMVVL
                     LNDGPLARELRRLGVSVTVVDESRTSALAILFFLIGFLKRNEIELVHTHRYKDTVLGF
                     IAARFAGVPHAVRTIHGLREPMKGWDRVKFGVYEALERVALHRWADLVIAVSKHIADD
                     LTGAGYPPAIVTHIHNGVDLRALARTRRRDEVRHDLGIDPTALVIGTAGRLVPVKGHA
                     SLLRAAGLILQKERRAKFLIVGDGPLRGELTALAVDLGIDGACQFLGHRADVHDLVSA
                     MDIFVLPSLAEGIPMAVLEAMALGSPVVATAVGGVPEVVGHRASGLLVESGEHAIADA
                     CLELAHDRDLAGRLAAEGRRVVEDRFSNVRSGEALVRAYERVALEERRPPHTRGLVRR
                     LARSLLNYASRRVAHAVGRQRMNRIRRNPELLTRAAQSARNLLIVCHGNIIRSPFAAQ
                     LLAKTLGDWRPRAIGSAGLEAVPGRPPHPTALVTATSHSVDLSRHTASPVTPERVAAS
                     DLIFVMDVPQLVTIQRRYPDARRKTFLLTCLAPEVPLEIHDPVDGDESMFKACFDHIA
                     RAASPLARALAAEGAAR"
     gene            complement(8060..8824)
                     /locus_tag="AC16CY_20240"
     CDS             complement(8060..8824)
                     /locus_tag="AC16CY_20240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3356193.1"
                     /translation="MLAHSNNVRTGTGRWLKAQEYEQAFWQRLGDDIESGTRGRLDWY
                     AWRATQLDSQLASAPDPPDRAGRVLEIGSGPVGIVNFLEWGERHAVDPLEHFYRTRPA
                     LVGLRKPGVSYFTGTGENLEFDDASFSLVIIDNVIDHTFEPGTIMREIGRVLRPDGYL
                     YLSVNVHTRWGALLHTALAVLQIDKGHPYTFTSRRLRALLSAHRFAIVAERVGDYAEA
                     VRENRRSPRWTDRIKGYTGLSEFSHGTLCRKQVQAS"
     gene            complement(8805..10067)
                     /locus_tag="AC16CY_20245"
     CDS             complement(8805..10067)
                     /locus_tag="AC16CY_20245"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HIR3356194.1"
                     /translation="MKVFVTDGDQRPALAITRSLGRRGVDVIVGEERPDSLASSSRYA
                     AAHVTYPSPYRQPQSFERWLLDFAARERVDVMIPVTDVTTHAVTRRQDVLRRHSALAV
                     PPFEAFDFVSDKGRLLERAATCGIPIPRTQVVSGLADVKRILAHVEFPAVVKPTRSRI
                     RTDHGWRSTTVSYAASPDELLRLYAEVDYLSSSPSLIQERIVGTGLGVFVICDHGRLL
                     TTFAHRRLREKPPSGGVSVLSESVPVDPQLRAQTMSLLGPIGWHGVAMAEYKQDERTG
                     RPVLMEVNGRFWGSTQLAIDAGVDFPFLSCQLALGRPPDLPPTYELGMKSRWLLGDLD
                     HLLIRLFEGPHQPARQTWSKMRAVVEFLGGTRGSRQEVFRRDDPRPMVYELTQYARAL
                     LRLRTIPAHHDVIGARVPFGGNHVGALE"
     gene            complement(10064..12061)
                     /locus_tag="AC16CY_20250"
     CDS             complement(10064..12061)
                     /locus_tag="AC16CY_20250"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR3356195.1"
                     /translation="MTPRVAFVGAGQMARQHRRAVMGLQIPSVVVGVHDSTPGRGRAF
                     AAAAGTQAYSSLDALLAEGRPDIVHVCTPPHSHVEVACAALDAGAHVYVEKPFALTAR
                     DARALLDLASSRRRIVCAGHQLLFDPAFQTLTKRVAELGAPLQVDSHFAFRPVAVSTT
                     RGSARALALQVLDILPHPLYSLIAVMEQSISDGERIDLAWVHAGPTSLHAVLRAGDLI
                     GRLSVSLRARPVASSISVTGTHGSLACDFVRSMVVGAANPGTEALEKVFTPMVEGAQL
                     VSRTIGSTLRRLRSGIDYPGLAELTGAFYRAVTQGGPPPVSGGHLIRVTEIFEALAAR
                     IEAAVERPAPRRSTPSCAAGTRVVVTGARGLLGAEVARALAPVRGIGRASACDDPNVS
                     EWVAADLSQGLHVDALTGADIVVHAAAETAGGYDDHRRNSIAATRHLMRTMHAAGVRK
                     LLLVSSLSVLAPPRTPWERQNERTPRASKPRPLGAYTWGKCLQEWLVERDAPKLGIEV
                     RIVRPGALMDWRDPALPGLMGRRLFGRWHLGLGRPGLPIAVCDVESCADVIASCVRHF
                     DRIPPVIHVFDPALPTRGALVARARTLGWRGRVVWIPISVVAVGMNAVRTMLSLLRLQ
                     WPASLAVWSILRPRRYDDVMATAVVRDARANMQQPTSEAAR"
     gene            complement(12058..13149)
                     /locus_tag="AC16CY_20255"
     CDS             complement(12058..13149)
                     /locus_tag="AC16CY_20255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3356196.1"
                     /translation="MTAHALDVAVDERDRTSAVAPDVHVTRVVAGECGEWRRLSRQLG
                     GHLAHAPEWSTVIQETYGHEPLYLLATDEVGPGGLLPAFIVRRPLIGAAVTSMPFLDG
                     GGPRSASPAMTTGLVNGLIAQAHRIGASVVELRCQERLPVAWQPMEHKVNQVLPLPPD
                     PEKLWHRLDRTVRNQVRKAHRSGLCVEFGGLERLDAFYAIFSNRMRELGSPVHDREFF
                     RSILTRFAGQARIAVVQNGATPIGGMLMLASNNVLTVPWASCLSRYSTLCPNMLLYWE
                     AIRAGCLEGRARFDFGRSTRGSGTHHFKRQWGADEIPLYWYTIPIKGRSTFSPGAGAT
                     SSRLADVWKRLPLSVTRHLGPRVRKYLIQ"
     gene            complement(13194..14348)
                     /locus_tag="AC16CY_20260"
     CDS             complement(13194..14348)
                     /locus_tag="AC16CY_20260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3356197.1"
                     /translation="MAEITFWVAVGLVFYAYIGYPLALLLLSRVYSREAKQAGEGRFT
                     PSVSFIIAVHNEEERIRSKIENTLAQDYPRHLLEIIVASDCSTDCTDGIVEEYSEQVR
                     LVRALERRGKEAAQELAVRAARGDILIFSDAATAIAPYGISRIVRNFSDPTVGCVSSI
                     DRSIDGDTIAGESAYVRYEMFLRTLETRVCGVVGLSGSFFAARREVCRRWAADRQSDF
                     NTVLRAAAVGLRSVLDQESVGYYRNVADPAREFQRKVRTVVRGMAVLASNLRLLNPFR
                     YGLFAWELASHKLCRWLVPLAMIAALLLNVVLFARSPLYQVMLILQLSFYAAALVGVW
                     TGARALRLPAFLCVANAAVLTAWLRFLRGDRFVSWNPSHRIGSLPSVGSR"
     gene            complement(14341..15582)
                     /locus_tag="AC16CY_20265"
     CDS             complement(14341..15582)
                     /locus_tag="AC16CY_20265"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3356198.1"
                     /translation="MRIGRSVPPAAAPFGWQDLGHAVAGAWSPVRAIHAREEEIREHF
                     GVPHVFLVSSGTAALTVTLAALKSLSSKTEVVIPAFTCFSVPAAVLKAGLRPVLCDID
                     PATFDFDHALLEQTLTDNTLCVVAHHLFAIPSDIARLKAMCQPRGIVVVEDAAQAMGV
                     RSGAGYLGTLGDVGIFSLGRGKTITCGSGGIVITRSDRIADAVHQRYLRLRFPSRAEM
                     WLDFVRILLMAVFIRPSLYWVPAAMPFLRLGETIFPKDVPLRLLSGLKAGFLRHWRRR
                     LHDSIQIRSETAADFSRQLSLHPVHGPAHPYLRLPVLAATPGDKDRIHALSRRLGLGM
                     SVAYPTSVNEIRQISAAFHGREFPNAKRVAEHLLTIPTHHWLSEADRCAIVTCLATAA
                     PDARPPGRWASPSSVEVVHHG"
     gene            complement(15597..>16998)
                     /locus_tag="AC16CY_20270"
     CDS             complement(15597..>16998)
                     /locus_tag="AC16CY_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356199.1"
                     /translation="GIGRVYLDGVMTEIDLYSTTEQVQATIFEATNLAAGSHTLVVES
                     TGTKNAQATDYAVVLDAIDIAPAVPPTTVGTRTEETAPSITFTAGWIGGDTTRAWSGG
                     TAASSGTAGARTTFTFTGTTVSWVGLREPGGGIARVFLDGAFQAQVDTYSPIDVQAVV
                     FTLTDLAPASHRLEIEVTGQRNPAATGSQIVVDAFDVRTRIEDADPSVVYTGTWSLHN
                     TDRNFSGTSQNTGGGTAARSATAGTRADFSFTGTSVSLVGFRGPWAGLADVFLDGAFA
                     TRVDLFSPGEQVQTPVFAAGGLAAGPHTLRIDVTGDKNPAATAALVHVDAFDITVPQP
                     APPVTRKQETDSSITYAGSWTASGTTPLWSGQTAVQATTSGAQATFTFTGTAIRWLGE
                     RGFATGVARVSVDGVFVAQVDTRTPLQEEYQAVLFSRTGLAPGSHTLTIDVIGRNGEP
                     PGVPVERVVVDAFDVY"
BASE COUNT         3005 a         5729 c         5698 g         2566 t
ORIGIN      
        1 gcgggaccag cgggcccgtc gtcgacgggt tcggcatgtg gagctggatg ttgccgccac
       61 cgggcccgcc gtcgcccagg aaccgaggtg tcacgaggta cccgttaccg aggcgaatac
      121 cgaaccggtc gtttcgtccg ggctcgccgt tgtcctccat gcgcacgcgg aaacggacgt
      181 actcgccggc tctggccgag gaattcgccg tgatcgacgt agttgacgtg gccccagaac
      241 gcgcccttct tgcacccgcc gtgggagccg aagttcgcca tggcgccggc gtcggtgaag
      301 atgaagccgc cgtcggagta acgtagtggc ggcggctgac ctcctttctc tgtgctgagt
      361 cgggggggct gtagccgggg cgggtgggcg caaccccttc ccgcccttca ttcctgaatt
      421 cgtgctttcc tacatcgttt gtgcatacat cgccgccctt tcgggcagcc gctgcggggt
      481 cagctgtggc cgcgtcatcg gctgccgtag caccgcggcg gcgacagctc tggcgacggt
      541 cttggacccc gatggcgtgg cgtggaagcc gtcgtagtag tcctcgaggc tcggctcgag
      601 gaggggcatc agatcaacgt gttcgaggtg cagctccttc gccacccgga cagccctcgc
      661 gtcgagcagc gccatgagtc gcgagcacac gtcgaaggag tagtaggtcg tcacgtcctc
      721 gcgccagacc cgccccgctc cgccgtgcca catgcacgcc acctcctcgg gcgtgaacac
      781 cttgtcgagc cacggctgcc gaacgacgag cacgcgatcg gcgtgcgcct gtgccttgcg
      841 gaggagcgcg cgaagatgga gctcgaagtg gtcgagcatc gaagagggat cgggcatcgt
      901 gtctcgaatc tccttcgcgc gcgcccgcat ggcccgcgct tgtgcgtccg ccttgcccgc
      961 gcgctcgtgg acctcgacgg gccgcagcca gcgccgccgc gccctccgca acagctcgaa
     1021 ggcggcgagc gcgcgcggtg tccagccgaa cggcccctcg gggtgacatc ggaagacgtc
     1081 tgccacgcgc gcccggcgcg gcggcgccgg cggggctcca tcctcgagcc atcgcagcac
     1141 gtcgctggcg ccgacgagca cgatgatcgt ggatagacgc gggtatcgcg gcagcacgcg
     1201 ctcgagaatg aggttgagcg cttcggatcc gacgcccgat cgagcgatgc tgccgacgtg
     1261 aacgtgcgtg gcgccaagcc tgcggagcgc ttccggccgt tcgagcagcc gttggagagc
     1321 gcctggccac gccgtgtact ggtcgagaag aaagccctcc ggttggctcc ctcctgcgac
     1381 gaggattcga tgcagccggt ccacggagcg ggggacctct ccgccacgtt caccgtcgcg
     1441 gttgacctcg aacctcgtct ctcgctccaa ctgtggaaag aggtcccgat cgatcttcag
     1501 gcgaagacgc aagcctggag ggaagacgaa gtagtcgccc ttgcggcgga tccagaatcg
     1561 ggccgccagt tccactgcga ctgacagcag cagggcgccg acgatgcccc atgtaagggt
     1621 gatcacatgt tcctccacgt caaccctgaa acctgaggga ccactcgccg tcgaacggtg
     1681 aaaaccaggc gtccccggtc tcgaacggtc gaacgagctc cggataccgc gccttcacca
     1741 gcacccatgg cccccggcga tgaaacaggc agtacctgtc ggacaacgcc tgcatgaatc
     1801 tgggctccat tctccctgtg gcggcaagcg cgccttcccg ccacgcgtga tgaaacagat
     1861 gatcgagcac gtcgtgaatc gaagacggtg tggcggcgac ctgcgccacc tcggcgacgc
     1921 cgtcgcgatc gagcaccgcg atgtaccagc ccgccatggt ccggccgtcc ttcacagcgc
     1981 ccgtgaggag ccgccgtgca ccgcgccggc ccgcggcgcg atccagcagc cactggaagt
     2041 tccgctcgtc gtgttcgacg cgaagcgctg cctcgcccaa ctgcggcacg ctggcgagga
     2101 cctcgtggac gtacagatcg cagggaggca ccgcgggccg ggactgccgg aaatgactgc
     2161 cggggagacg cgccgcacag gcgtcggcaa gccgcgcaaa cggttgcgcc accgtcgcga
     2221 gcggcgcgaa cgggcggcgc ccgcgcagat acgacaacgc cagcccggcg ggacgcaggg
     2281 gacgcgtcca atacaggctg agcagacgag ccgtcgtgcc gcccagccct tcccagatga
     2341 tccgggacac gtcgttcgcc tcgtcggcga tcgacacgtc ctggggcccc tcgagaaacg
     2401 tcttactgag cttgagggcc accagaccga cggcgctggc gggatccacg atgaactgcg
     2461 agctgacggc tgcctcgacg tgccggccac ccatggccat gcggcgcggg acgatgccca
     2521 ggaacccgac gatgcgcccg tcgctctcgt gatacacgag cgaacgtgcc gggccccttt
     2581 gggccggatt ctcgaggaag acctccgtga agtagccgcg atacgagcgt tggacgccga
     2641 cgccgtccgg ccagcagcgc tgatgcagcg cggccacctg cgggatgtcc gcctcttcga
     2701 atcgacggac gccggccgtc ctgtgagcgt tcacccgtcg aacctgcgcc agtgcagcac
     2761 tcatctgtga ccagtcgtca agaacagccc gcgtgcggat ttgaccaagg ccgctgcgac
     2821 cgtgcgggtt gcgtgtcccg cgcgcgcgaa ctgcgaggcc ggctgcgccg agtagacgtc
     2881 ggcgtccatc gtgatccgat cgcggaccgg ccagaggtat ttgacgcgct cgacgccctt
     2941 gaggaagtcg aactccatgg cgccctcgcg tgccgcggcg tcaatggccg cagcaagcgt
     3001 gagttgcccg aggtgtatcc gtcccgccca ttcccggtcg tagccggcca ggtagtagcc
     3061 ccaccaccgc gcgctcgcga tcccgtagaa gaccgcgacc gtacggagat cggaggtcac
     3121 gcgcagcatc ctgagccggc cttccccgag cagcagcggc aggacgtgcc ggtggaaccg
     3181 ctgcgcgcgc ggatcgtcga gcgccgatcg gtgcgttgcg ccctgccacc gcgctcgcag
     3241 aaacccgacg agcgactcga acgccgagat gacgtctcct cgctcccgga gcacctcgat
     3301 cgtcaatcga ccgcgcccct cgagccatcg gcgatgcttg ccgagcgtgc gggccagaga
     3361 ctctgaagcc agtctgccgt cgtagagaga cttcagatcg atgatgtcga ggtgtggcag
     3421 cgtgtacacc tctcgcggct cgagcacgag ccgcagcgtc ccctcctgag cggtccggtg
     3481 aactgcgccc cagagcgccg aactgtcggc ggcgtcccgc aactcgagga cgaagccccg
     3541 gcggccgaac gactcaccca gccacgcgag gaagcgatcc gcgcacgccg gctcgaagcc
     3601 ccgctcggca aggaggtcga tccgatccga ccccaggcct cccccgagca ggctcacgtg
     3661 gaccagcgtg ttcctacgac ggaccgcaat cgggacgatg cctcgaaggg tcgttccctc
     3721 ccggagcatc aacaggaacg gctccgagcc gggcggcggc tcggcgaaga agccggacag
     3781 ccaggcccgc gagagaaaca cgccgacgtg cggccggctg tcgatcagcg cgccgagtgt
     3841 gacgtcgtct tccggcgcca gctcgagatc gcgcttcagc tcgatggcga gaggcgcgcg
     3901 cccggaagcg cgtgcgtgcc ggggcggggc tggcagatcg acggccgcag gatttcctct
     3961 cacgcgcgac cccatgcccg catcacgagg agtttctgcg ctcgcacatc cacgtaggga
     4021 tcgcctcggt acacgaagcc gatccagacg agcccgaaca ggagaaccat gacgatccct
     4081 gcgacgagca gaccggtcaa gctctgtacc tgcaacccca gcctgaacca gagcagcaac
     4141 gccaggaccg gcagggctcc aagcgtggct ctgggcacga cgtagcgggt ccagtgcgcc
     4201 acgctgacgt cgagttcccg gcagacgacc gtcaacacca cgagggagaa gaggacgttc
     4261 ggaatcgctg tcccgagagc gacacctgcc agcccgagcg gcccgatgag ggccatgctc
     4321 aggccgacgt tcagcagacc ggcggcgata aaggcgaggg ccggcatcct cggcttccca
     4381 aggcccatga gaatgggcac ggccacgccg cgcacgggta agaagatgaa gctcgacacc
     4441 atgaggatct gcagcacgcc gccggccggc tgctcgtacg acggatcgat ccaccaggcg
     4501 atgaacctcg gcccgagcac gcacaggaag agtccggcca tgatcgtcag cgacaaggcc
     4561 actttcgacc acttcaggaa catcgccctg acctcgtcga tgcgtccctc ggtgttcagc
     4621 ttcgtcgtca tgggcgagac gaccgctgca atcgacgtca tgaagtcgat caggtagacg
     4681 atgaggctgt tggcgacgac gtagaacgga atcgcgccga cgcccatgaa ggcgccgatc
     4741 accagcgcgt cgctctcgaa gctcagacgc gcgccggcgg tgagcagcag gacccagaca
     4801 ctgaaggaga agatttcccg cacgaccccg agattgaacc cggcgaggcc gatgcgcacc
     4861 tccggatacc gccggctgat gatgagccac gacacgaaga aatcgaacgc gagacaggtg
     4921 agctgaatcg aggccagggc gacgagggag gcgtcgagcg ccagcagtcc aatcgtgagg
     4981 cccgtgcgca gcagaacgcc ggccatccga atgacgttcc gccgcacgaa gtcgtggtgc
     5041 gcgaacagga tgccttccgg caggaagccc atgaagctgg ccgagacggt cacgaccatc
     5101 acccagaagg ccagcagcga ctcggccttc cacccgtccg gaatcgagta ctgctgaaag
     5161 aacagcagca gcagcgctcc cagcacggcg gccacgacgc cgacgacgcc atacagaccg
     5221 acgcagctgc cgatcgtctg gttcatcctg cgcgtgtcgc ccgccgcgac gtgctgcgac
     5281 aggtaccgaa cgcacgccat cggcacgcca agggcgagca ggctgatatg tccggtgagc
     5341 gtcgtgatga gcgtccacgt cccgtaaccc tcttcaccga gtgcgtggat gatgaagggc
     5401 gtgaggaggt agctcgccgc aatcgtgacg aggatcagcg cccagttgct tccgacattg
     5461 cgcagcatgg ttggtgtgga tggggtgcgc gcctccggac ggccccgctc aggcggcctg
     5521 aagggtgcga cctagcagga cttcgaatcc gaactgttgt ggcgtcgtgc accacggaga
     5581 catggccact cggcgcacgt cgaacggact acgacccggc ccgttgatcc ctccgaaaac
     5641 ggagcagcag cagcggtagc cggcatcctt cacgagctct cggttcgcct cggtgaggtt
     5701 gtggcggcct ccgtacggat acgcgaagga cgcgaccggc gccccgagcc gctcctccag
     5761 ctcgatccgc gcgccctgaa tctcccgtcg ggctgtgacg tcgcgcacga ggccgagatc
     5821 ggcgtgcgtc cgcgtatgcg cgccgatgtc gaagccccgc cggtgcagcg accgcacctc
     5881 gtcccacgtc atccaggggt atcgacggcc gtgccgccgg tcccaccacg ggacgacgtc
     5941 ggtgcccatc cactgcgtga cgacgaagaa ggtcgcgggc agggagcatc gctccaggat
     6001 cggcgccgcg tgctcgaagt tgtcccgata gccatcgtcg aacgtgatga cgagccttcg
     6061 attgagatcc tgattgcgct cgagcctcgt cacgagctcc tgcaggggaa tcacgtggaa
     6121 gtagcgctgg aagaaccggc agtatcgttc gaacagatcg aggtcgatgg tgagcccgct
     6181 gggccgctcg cggcgcgcgc cgaccgggaa cacacggtga aacgccacga ccacggcggc
     6241 gtccttcagc aagagctggt ggaggcgcga ggtaaacaga gtctggccga gtgccgcctt
     6301 cacgagtcgt ctcatcgagc cgcaccctcc gccgcaaggg cgcgcgcgag cgggctggcg
     6361 gctcgggcga tatggtcgaa gcaggccttg aacatcgatt cgtcgccgtc gaccgggtcg
     6421 tggatttcga gcggaacctc cggcgccaga cacgtcagga gaaaggtctt tctgcgagcg
     6481 tccgggtagc gccgttgaat ggtcacgagc tgtggcacgt ccatcacgaa gatcaggtcg
     6541 ctggccgcca cccgttccgg ggtgactggc gaggcggtgt ggcgactcag atcgaccgag
     6601 tgcgacgtgg ccgtgaccag cgccgtcggg tgaggcggcc ggccgggcac cgcctcgagg
     6661 cctgcggagc cgatggcgcg cggcctccag tcgccgagcg ttttcgcgag cagctgtgcc
     6721 gcaaaggggc tcctgatgat gttgccgtgg cacacgatga ggaggttcct cgcggattgt
     6781 gcggcgcgcg tcaggagctc cgggttacga cgaatccgat tcatccgctg ccggccgacg
     6841 gcgtgcgcca cgcggcgcga tgcatagttg agcagactgc gcgcaagccg ccggacgaga
     6901 ccacgcgtgt gagggggacg tcgctcctcg agagccactc gttcgtacgc gcggacgaga
     6961 gcctcgccgc tccgcacgtt cgagaagcgg tcttcgacga cgcgccgacc ctcagccgca
     7021 agacgccccg ccaggtcgcg atcgtgcgcc agttcgaggc aggcgtcggc gattgcgtgt
     7081 tctccggatt ccaccagcag accgcttgcg cgatgaccga cgacttccgg cactccgccg
     7141 acggcagttg ccacgacggg gctgccgagc gccatcgcct ccagaacggc cattgggatc
     7201 ccctccgcaa gcgatggcag cacgaagatg tccatcgcag acacgaggtc gtgcacgtcc
     7261 gcccggtggc ccagaaactg gcaggcaccg tcgatcccga gatccacggc cagcgccgtc
     7321 aactccccgc gcagcggacc gtcgccgacg atgaggaact tcgcacgccg ctccttctgc
     7381 agaatcagac cggccgcccg cagcaggctt gcgtgcccct tgaccggcac cagccttccg
     7441 gccgtgccaa tgaccagagc cgtggggtca atgcccaggt cgtgccggac ctcgtctcgt
     7501 cgtcgcgttc gcgccagggc acggagatcg acgccgttgt ggatgtgcgt cacgatcgcc
     7561 ggcgggtatc cggctcccgt gaggtcgtcc gcgatgtgct tcgacacggc gatcaccagg
     7621 tccgcccagc gatgcagcgc gactctctcg agcgcttcgt agacgccgaa cttgacccgg
     7681 tcccagccct tcatcggctc gcgcaggccg tgaatcgtcc gtacggcgtg cggcacaccg
     7741 gcaaaccttg cagcgatgaa cccgagcacc gtgtccttgt accggtgcgt gtgcacgagc
     7801 tcgatctcgt tccgcttcag aaacccgatc aggaagaaca ggatggcaag ggcgctcgtc
     7861 ctcgactcgt cgacgacggt cacggagacg ccgagccgcc ggagctcccg ggccagcggt
     7921 ccgtcgttca gcagcaccac catcaggctg atgcccgggt cgccgacgag ataggcaacc
     7981 gtcgaggcga cctgcacttc cgctcccgcc cacaggtcgg cggacatgac gtggcagacg
     8041 cgcagcttcc tgccctcggt cacgacgctt gaacctgttt gcgacagaga gtgccgtgcg
     8101 agaactccga gaggccggta tagcccttga tacgatcggt ccatctcggc gatctgcggt
     8161 tctcgcgcac agcctcggcg tagtccccta cgcgctcggc gacgatcgcg aaccggtgcg
     8221 cgctcaggag cgctcgcaat ctcctgctgg tgaaggtgta ggggtggccc ttgtcgatct
     8281 gcagcaccgc gagggcggtg tgcagcaacg ctccccatcg cgtgtggacg ttgaccgaca
     8341 agtagaggta cccgtcaggc ctgaggacac gcccaatctc ccgcatgatc gtcccgggtt
     8401 cgaaggtgtg gtcgatgacg ttgtcgatga tgacgagaga gaacgaggcg tcgtcgaact
     8461 cgaggttttc gccggtcccc gtgaagtagc tcacgcccgg cttcctcagc ccgacgaggg
     8521 ccggcctcgt gcggtagaag tgctcgagcg gatccacggc atggcgctct ccccactcga
     8581 ggaagttcac gatgccaacc gggccgctgc cgatctcgag cactcgaccg gcgcgatcgg
     8641 gcggatctgg agccgatgcg agctgcgagt cgagctgagt cgcccgccac gcgtaccagt
     8701 cgagccgccc ccgcgttcca gactcgatgt cgtcgccgag ccgttgccag aacgcctgtt
     8761 cgtactcctg agccttgagc cagcgtccgg ttccggtccg gacgttattc gagtgcgcca
     8821 acatggttgc ctccgaacgg cacgcgcgcg ccgatgacgt cgtggtgggc agggatggtc
     8881 cgcagcctga ggagtgccct cgcgtactgc gtcagctcgt agaccatggg gcgaggatcg
     8941 tcgcgtcgga acacctcctg ccgcgagccc cgggttccac cgaggaattc gacgaccgcg
     9001 cgcatcttcg accacgtctg tcgcgcgggc tgatgcggtc cctcgaacag acggatcaga
     9061 agatgatcca gatcgccgag cagccagcgg ctcttcatcc ccagctcgta ggtcggcggg
     9121 aggtccggcg gtcgtcccag cgcgagctga caggagagga aggggaaatc cacaccggca
     9181 tcgatcgcca gttgcgtcga cccccagaag cgaccgttga cctccatcag caccggccgt
     9241 cctgtgcgct cgtcctgttt gtattccgcc atcgccactc cgtgccagcc gatcggccca
     9301 agcaggctca tcgtctgcgc gcgaagttgt ggatccacgg ggacgctctc gctgagcacg
     9361 ctcacgccgc ccgacggcgg cttctcacgg agtcgccggt gtgcaaatgt cgtcagcagc
     9421 cggccgtgat cgcagatcac gaagacgcca aggccggttc ccacgatccg ctcctggatc
     9481 agcgacggag aggacgagag gtagtcgacc tccgcgtaga gccgcagcag ctcgtccggg
     9541 gaggcggcgt agctcaccgt cgtcgatcgc cagccgtggt ccgtgcgtat cctcgaccgg
     9601 gtcggcttga ccacggccgg gaactcgacg tgcgccagaa tgcgtttcac gtcggccagc
     9661 cccgagacga cttgcgtccg agggattggt atcccgcatg tcgccgcacg ctcgaggagt
     9721 cgtcctttat ccgacacgaa atcgaacgcc tcgaacgggg gaaccgcgag cgcactgtgc
     9781 cgtcggagga cgtcctgcct ccgcgtcacg gcatgcgtcg tgacgtccgt cacgggaatc
     9841 atgacgtcca cgcgctcccg cgcggcgaag tcgagcagcc atcgttcgaa tgattgcggc
     9901 tggcggtacg gcgagggata ggtcacgtgg gccgcggcgt acctggacga cgaggccaga
     9961 ctgtccggac gctcctcgcc gacgatcacg tcgacgcccc gccggccgag cgagcgcgta
    10021 atggcgaggg caggccgttg atcaccgtcg gtgacgaaca ctttcatctg gctgcctcac
    10081 tcgtcggttg ttgcatgttt gcacgcgcgt cccgcacgac cgcggtggcc atcacgtcgt
    10141 cgtagcgtcg cgggcgcagg atcgaccaga ccgcgagact ggccggccac tgcagccgga
    10201 ggagagacag catcgtgcgc acggcgttca tccccacggc caccacgctg atggggatcc
    10261 acacgacgcg gccgcgccag ccgagcgtgc gcgcgcgggc cacaagcgcg ccgcgcgtcg
    10321 gcaatgctgg atcgaagacg tgaatcaccg gaggtatccg gtcgaagtgc ctcacgcacg
    10381 aggcgatgac atcggcgcag ctttcgacat cgcagacggc aatcggcaga ccgggccggc
    10441 ccagacccag atgccagcgg ccgaagaggc ggcgacccat cagccccggc agcgccgggt
    10501 cgcgccagtc catcagcgct cccggccgca cgatgcgcac ctcgatgccg agcttcggcg
    10561 cgtcgcgttc gacgagccat tcctgcaggc atttgcccca cgtatacgcg ccgagcggcc
    10621 tgggtttcga cgcccggggc gtgcgctcgt tctgccgctc ccacggcgtg cgcggcggcg
    10681 caagcaccga gaggctgctg accagaagga gcttgcggac gcctgccgca tgcatcgtgc
    10741 gcatcaggtg ccgcgtggcc gcgatcgagt tacggcgatg atcgtcgtac ccgccggcgg
    10801 tctccgctgc ggcgtgcacc acgatgtcgg ccccggtcag cgcgtcgacg tggagcccct
    10861 ggctcaggtc ggcggccacc cactcgctca cgttcgggtc gtcgcatgcg ctggctcgcc
    10921 cgatgcctcg aacgggagcg agtgcccgtg ccacttctgc cccgagcagg ccgcgcgcgc
    10981 cggtgacgac gacccgggtt ccggcggcgc aggacggagt cgatcggcgc ggggccggac
    11041 gttcaaccgc cgcctcgatt ctcgcggcga gcgcttcgaa gatctcggtg acgcggatca
    11101 ggtgcccgcc tgacacgggc ggcggtcctc cctgggtgac ggcgcgatag aacgcacctg
    11161 tgagctcggc cagccccgga tagtcaatgc ccgaacgcag ccggcgcagc gtgctcccga
    11221 tcgtcctcga cacgagctgt gcgccttcca ccatgggggt gaagaccttc tcgagtgctt
    11281 ccgtgccggg gttcgcggca cccacgacca tcgagcgaac gaagtcgcag gccagcgatc
    11341 catgcgtgcc ggtgacgctg atcgacgagg cgaccggcct ggcccgaagg ctgaccgaca
    11401 ggcgcccgat cagatcgccg gcacgcagga ccgcgtggag agaagtcggc ccggcgtgca
    11461 cccaggcgag gtcgatgcgc tcgccgtctg agatcgattg ctccatcaca gcgatgagcg
    11521 agtagagagg gtgcggaagg atgtcgagga cctgcagggc aagcgcccga gcgctgccgc
    11581 gggtcgtgga cacggcgacg ggccggaagg caaagtgact gtcgacctgt agcggggctc
    11641 ccagctccgc cacccgcttc gtcagggtct ggaacgccgg gtcgaagagc agctgatgcc
    11701 ccgcgcacac gatccgccgc cgggacgagg cgagatcgag caacgccctg gcatcacgcg
    11761 cggtgagggc gaatggcttc tcgacataga cgtgcgcccc cgcgtcgagc gccgcgcaag
    11821 ccacttcgac atgcgaatgg ggcggcgtgc agacgtgaac gatatcgggc cgaccctcag
    11881 cgagcagggc atcgagcgag gaataggcct gcgttccagc cgccgcggca aacgctctcc
    11941 cgcggccggg agtggaatcg tggacgccca ccacgacgga cgggatctgc aggcccatga
    12001 cagcccggcg gtgctggcgg gccatctggc ccgcgccgac gaaagccaca cgcggtgtca
    12061 ttgaatgagg tacttccgga cacgaggtcc gagatgccgt gtcaccgaga gcggcaggcg
    12121 cttccataca tcggctagac gagaggacgt cgcgcccgcg ccgggggaga aggtgctccg
    12181 ccccttgata ggaatcgtgt accagtagag cggaatctcg tccgcacccc attgacgctt
    12241 gaagtgatgg gtccccgagc cccgagtcga tcggccgaaa tcgaaccgcg cgcgtccctc
    12301 caggcacccc gcccggatcg cctcccaata gagcagcatg ttcgggcaca gcgtggagta
    12361 ccgcgacaga cacgacgccc agggcacggt gagaacgttg ttggaggcga gcatgagcat
    12421 tccgccgatc ggtgtggcgc cgttctgcac gacggcgatc cgcgcctggc ccgcgaagcg
    12481 cgtgaggatg gaccggaaga actcgcgatc gtgcaccggc gaaccgagct cgcgcatacg
    12541 gttcgagaag atcgcgtaga aggcatccag gcgctccagt cccccgaact cgacgcacag
    12601 gccagaccgg tgcgccttcc gaacctggtt cctgacggtg cggtcgagcc gatgccacaa
    12661 cttctccgga tcgggcggca gaggcagaac ctggttcacc ttgtgctcca tcggttgcca
    12721 ggcgaccggc agcctctcct ggcagcgcag ctccacgacg ctggcgccga tccgatgagc
    12781 ctgcgcaatg agcccgttga cgagcccggt ggtcatcgcc ggggaggcgg accgggggcc
    12841 cccgccatcc aggaacggca tggatgtcac tgccgccccg atcagtggcc gccgcacgat
    12901 gaacgccggg agcaggcctc cgggtccgac ctcgtccgtg gccagcaggt agaggggctc
    12961 gtgcccgtac gtctcttgaa tcacggtgct ccactccggc gcatgggcca ggtgtccccc
    13021 gagttgccgg gagagccggc gccactctcc gcactcgccg gcgaccacgc gtgtgacgtg
    13081 cacatcgggt gcgacggcgc tggtgcggtc gcgttcgtcg acagcaacgt caagggcgtg
    13141 ggcagtcata cgccatcacc tcgtgttggg ggggactaat cagctcgacg cgactagcgc
    13201 gagccgacag aaggaagcga gccgatgcga tgcgaggggt tccaggacac gaagcgatct
    13261 cctcgaagga accggagcca tgccgtcagg acagcggcgt tcgccacgca gagaaaggcc
    13321 ggcaggcgca gcgctcgagc cccggtccac accccgacga gcgccgccgc atagaagctc
    13381 agttgaagaa tcagcatcac ctgatagagc ggtgaacgcg caaacagcac cacgttcagg
    13441 agaagggccg cgatcatggc cagcggcacg agccagcggc atagcttgtg gctggcgagc
    13501 tcccaggcga aaaggccgta tcgaaacggg ttcagcaatc gaagattgga tgcgagcacg
    13561 gccatgcccc gaacgacggt cctgaccttt cgctgaaact cgcgcgccgg atccgcgacg
    13621 ttccggtagt agccgacact ctcctggtcc agcacgctcc gcaggcccac ggccgccgcc
    13681 ctcaggaccg tgttgaaatc gctctgacga tcggccgccc accggcggca gacctcgcgg
    13741 cgcgccgcga agaacgaccc gctgagcccc acgacgccgc agactcgcgt ctcgagcgtc
    13801 cggaggaaca tctcgtatcg gacataggcg ctctcgcccg cgatggtgtc accgtcgatc
    13861 gaccgatcga tgctgctgac gcagccgacg gtgggatccg agaagttcct gacgatgcgc
    13921 gagatgccgt agggcgcaat agcggtggcc gcgtcggaga agatcaggat gtccccccgc
    13981 gcggcccgca cggccaactc ctgcgcggcc tccttgccac gacgctccag cgcacgaacg
    14041 agccgcacct gttccgagta ctcctcgaca atcccgtcag tgcagtcggt cgagcagtcg
    14101 gaggccacga tgatctccag cagatggcgt ggatagtcct gggcgagggt gttctcgatc
    14161 ttgctgcgaa tgcgctcttc ctcgttgtgg accgcgatga tgaatgagac ggagggggtg
    14221 aagcgaccct cgccggcctg tttcgcctcc cggctgtaca cgcgcgacag gagcaggagc
    14281 gcgagcgggt agccgatgta ggcgtagaac acgagaccga ccgcaaccca gaacgtgatt
    14341 tcagccatga tgcacgacct cgactgaaga cgggctcgcc catcggcctg gagggcgagc
    14401 gtcgggcgcc gccgttgcga gacacgtgac gatggcgcac ctgtctgcct ccgaaagcca
    14461 gtggtgcgtc gggatcgtca gcaggtgttc cgccaccctc ttggcgttgg gaaactccct
    14521 gccgtggaac gccgcgctga tctgccgaat ttcgttgacc gacgtgggat acgcgacgct
    14581 catgccgagt cccagccgcc gggagagcgc gtggatgcgg tccttgtcgc ccggcgtggc
    14641 ggcaagaacg ggtaaccgga gataggggtg cgccggccca tggaccgggt ggagcgacag
    14701 ttgccgactg aagtcggccg ccgtctccga tctgatctga atcgagtcat gaagacgtcg
    14761 tcgccagtga cgcaggaacc cggccttcaa acccgacagg agacgaagcg gcacgtcctt
    14821 cggaaaaatg gtctcgccca gccgcaggaa cggcatcgcc gccggaaccc agtagaggct
    14881 cggtcggatg aagacggcca tcaggagtat ccggacgaag tccagccaca tctcggcacg
    14941 cgagggaaag cgcaggcgca ggtatcgctg gtggacggca tcggcaatcc ggtcgctgcg
    15001 cgtgatcacg atgccgcccg acccgcacgt gatcgtcttg ccacggccga ggctgaagat
    15061 tccgacgtcg ccgagcgtgc cgagatagcc ggctccggac cgcacgccca tcgcctgggc
    15121 cgcgtcttcg accacgacaa tgccgcgcgg ctggcacatc gctttgaggc gtgcgatatc
    15181 ggagggaatc gcgaacagat ggtgcgccac gacgcacagg gtgttgtccg tcagcgtctg
    15241 ctcgaggagg gcgtggtcga aatcgaacgt cgctggatcg atgtcgcaca ggaccggacg
    15301 caacccggct ttcagcaccg cggccggtac cgagaagcag gtgaacgcgg ggatgacgac
    15361 ctcggtcttc gaggacagcg acttgagcgc cgccaacgtc acggtcaacg cggcggtacc
    15421 agacgagacc aggaagacat ggggcacgcc aaagtgctcc ctgatttcct cttcgcgcgc
    15481 gtgaatggcg cgcacgggag accaggcgcc ggcaacggcg tgaccgaggt cctgccagcc
    15541 gaacggggcg gcggcaggag gcaccgagcg accgattctc atggcggaat ctgctgtcaa
    15601 tacacgtcga atgcgtctac gacgacccgc tccacgggca cgcccggcgg ctcgccgttt
    15661 cggccgatca cgtcgatcgt cagcgtgtgg ctgccgggcg cgaggccggt cctgctgaac
    15721 aggacggcct ggtactcctc ttgaaggggc gtgcgggtgt ctacctgcgc cacgaagacg
    15781 ccgtccacgc tcacgcgggc gacgcccgtc gcaaaacctc gttccccgag ccacctgatt
    15841 gcagtgcccg tgaaggtgaa ggtggcctgc gcgccggacg tcgtcgcctg tacggcggtt
    15901 tgcccgctcc agagcggagt ggtgccactg gccgtccacg atccggcata cgtgatcgat
    15961 gaatcggtct cctgtttgcg cgtcacggga ggcgcgggct ggggcacggt gatgtcgaat
    16021 gcgtcgacgt ggacgagggc cgctgtcgct gctgggttct tgtcgccggt cacgtcgatc
    16081 ctcaacgtgt gcggcccggc ggccagccca ccggcggcga agacgggcgt ctggacctgt
    16141 tcacccggcg aaaacagatc cacgcgcgtg gcgaacgcac cgtcgagaaa cacgtcggcc
    16201 aggccggccc atggcccgcg gaacccgacc aggctcaccg aggttcccgt gaacgagaag
    16261 tccgcgcgcg tgcccgcggt cgcgctgcgt gctgccgtgc ctccacctgt gttctgcgac
    16321 gtgccgctga agttgcggtc ggtgttgtgg aggctccacg tgccggtgta cacgacagac
    16381 ggatcggcat cctcgatgcg ggttcgcaca tcgaaggcgt ctaccacgat ctggctcccg
    16441 gtggcggccg ggttccgctg acccgtgacc tcgatctcca accggtgact ggcgggggcg
    16501 agatcggtca gggtgaagac aaccgcctgc acgtcgattg gggaatacgt gtcgacctga
    16561 gcctggaacg ctccgtcgag gaagacgcgc gcgatgccgc cgccaggttc tcgcaggcca
    16621 acccaactga ccgtcgttcc tgtgaacgtg aacgtggtgc gcgcgccagc ggtgccggag
    16681 gatgccgccg tgcctccgct ccatgccctc gtcgtgtcgc cgccaatcca ccccgcggtg
    16741 aacgtgatgg acggagccgt ttcctccgta cgggtcccta ccgtcgtcgg cggcaccgcc
    16801 ggtgcgatat cgatcgcatc gagcacgacg gcgtagtcgg tcgcctgcgc gttcttggtg
    16861 ccggtcgatt ccacgaccag cgtgtgactg ccggccgcca aattggtggc ctcgaagatc
    16921 gtggcctgca cctgctcggt ggtcgaatac aggtcgatct cggtcatgac gccgtccagg
    16981 tagacgcggc cgatacct
//
LOCUS       DBNGSH010000882         1549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_109327, whole genome shotgun sequence.
ACCESSION   DBNGSH010000882 DBNGSH010000000
VERSION     DBNGSH010000882.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_109327"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..>1549
                     /gene="guaA"
                     /locus_tag="AC16CY_20275"
     CDS             21..>1549
                     /gene="guaA"
                     /locus_tag="AC16CY_20275"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR3356200.1"
                     /translation="MSHQTILVLDFGSQYTQLIARRLRELSVYSEIVPFNTPLSKLEQ
                     KRPVGLILSGGPSSVSDEGAPACDARLFELGIPVLGICYGMQLMTRTLGGEVRRSGNR
                     EFGHALVRVCRNGGQATHPPPRLFHEVPSELRVWASHGDDVAAVPPGFAVAATSATAP
                     IAAMEAPERHLYALLFHPEVAHTDHGVAILRNFAYGECGCTGDWTISSFIEESTDRIR
                     RQVGDGQVVCGLSGGVDSTVAAMIIHGAIGDRLTCIFVDNGLLRYEEANQIQKRFRDK
                     LRLPLDFVDASSLFLDRLAGVVDPEQKRKIIGATFIDVFEKRAKELGEFGFLAQGTLY
                     PDVIESASVHGPAVVIKSHHNVGGLPERMKFALVEPLRDLFKDEVRRVGRDLGLDREF
                     LVRQPFPGPGLAVRVVGEITKPRLDMLRLADRIVADEIRRAGWYERLWQSFAVLLPVQ
                     SVGVMGDSRTYEYTIAIRAVESLDGMTADWARLPHELLAAISSRIVNEVRGINRVVYD
                     IS"
BASE COUNT          250 a          496 c          511 g          292 t
ORIGIN      
        1 cgccgcaagg tcacctcatc gtgtcgcatc agaccatcct cgttctcgac ttcggctcgc
       61 agtacacgca gctgattgcg cgccggctgc gcgagctgtc ggtgtattcc gaaatcgttc
      121 cgttcaacac gccgctgtcg aagctcgagc agaagcgacc cgttggactc atcctttccg
      181 gcggaccgag cagcgtgtcg gacgaaggtg cgccggcatg cgacgcgcgc ctgttcgagc
      241 tcggcattcc tgtcctgggc atctgctacg gcatgcaact gatgacgagg acgctgggcg
      301 gcgaggtcag gcgctcgggc aatcgcgaat tcgggcacgc gctcgtccgc gtctgccgca
      361 atggcggcca agccactcat ccgccgccgc ggctgtttca cgaggtgccg tcggaactgc
      421 gcgtctgggc gagtcatggc gacgacgtgg ccgccgttcc gcccggtttc gccgtcgcgg
      481 caaccagcgc aacagcgccc atagcggcga tggaggcccc ggaacggcac ctctacgcgc
      541 tgctcttcca cccggaagtg gcgcacacgg atcacggcgt tgcgattttg cggaattttg
      601 cctacggaga atgcggctgc accggcgatt ggacgatttc gtccttcatc gaggagtcta
      661 ccgatcggat ccgtcgccag gtgggggacg gccaggtggt ctgcggattg tccggcggcg
      721 tggattcgac tgttgccgcc atgatcattc acggcgcaat cggcgatcgg ctgacgtgca
      781 tcttcgtgga caacgggttg ctgcgctacg aggaagccaa tcagatccag aagcgattcc
      841 gcgacaagct gcggctgccg ctcgacttcg ttgacgcgag ctcgctcttt ctggaccgtc
      901 tcgccggcgt cgtcgatccg gagcagaagc gcaagatcat cggcgcgacc ttcatcgacg
      961 tgttcgagaa acgcgcgaag gagctcggcg aattcggttt cctcgcgcag gggacgctct
     1021 acccggacgt gatcgaatca gcgtcggtgc acggcccggc cgtcgtcatc aagagccacc
     1081 acaacgtcgg cgggctcccg gagcggatga agtttgccct tgtggagccg cttcgggatc
     1141 tgttcaagga cgaggtgcgg cgggtcggcc gcgacctcgg gctggaccgc gagttcctcg
     1201 tgcgccagcc gtttccaggg cccggcctgg ccgtgcgcgt cgtcggcgag attacgaagc
     1261 cccgcctcga catgctgcgc cttgccgatc gcattgttgc cgacgagatc cggcgcgccg
     1321 gctggtacga gcggctctgg cagagtttcg cggtcctgct gcccgtccag agcgtcggcg
     1381 tcatgggtga ttcgaggacg tacgagtaca ccatcgccat ccgggcagtc gaaagccttg
     1441 acggcatgac ggctgactgg gcgcgcctgc ctcacgaact gctcgcggcc atctcgtcac
     1501 ggatcgtcaa cgaggttcgt ggtatcaacc gggtggtgta cgacatctc
//
LOCUS       DBNGSH010000883         9151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3844012, whole genome shotgun sequence.
ACCESSION   DBNGSH010000883 DBNGSH010000000
VERSION     DBNGSH010000883.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3844012"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="AC16CY_20280"
     CDS             complement(<1..1165)
                     /locus_tag="AC16CY_20280"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3356201.1"
                     /translation="MSARVLAYVGSSLLVYASISSAAESDARLAEAARREDRAAIRTL
                     LAQKAEVNAPLADGSTALHWAVQADDLETVNLLLEAGANATLANRFGVTPLHVATSNG
                     NSAILARLLRAGADPDARDASGETLLVMAVRAGNLDSVASLVERGASVNAADTATKQT
                     PLMWAVRSNQAAAAKLLIEHGADVNAATRVGATPQWTPPNAGGGSHGLGIIRGGWPER
                     GSRAAVPGAMTALLYAARDGHLPLARLLLDARADVNLAEANAISPLLMAITNDHMDVA
                     RLLVERGADVNAADFWGRTPLFSAVEIRNRDFTRGNEHGIDRAAALEMVKLLLDRGAN
                     PNARTKEIPPIRRWVTSLGDLTWINMIGQTPFIRASMSGDITTMRLLLERGADP"
     gene            1201..1863
                     /locus_tag="AC16CY_20285"
     CDS             1201..1863
                     /locus_tag="AC16CY_20285"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR3356202.1"
                     /translation="MNTTARFGRVVALGAIAWMALCFPAPLQSQAPTGSTRIEITAGS
                     TAEYRVREQLARLNFPNDAVGRTDSLTGAIVVGPGGAIAPGSKLTVDLRDLQSDADRR
                     DNFLRQNTLHTSKFPMAEFVPRRQEGLPSPIPSSGKAGFKLIGDMTVHGVTTELAWDV
                     AATFAPGAVSGEATTRFQFAKFGLAIPKVMGLLSVDDDIRLVLNFKARRVEVPAVASS
                     SN"
     gene            complement(1897..2457)
                     /locus_tag="AC16CY_20290"
     CDS             complement(1897..2457)
                     /locus_tag="AC16CY_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIR3356203.1"
                     /translation="MLRAEAPKLKTYIALFRAINVGGKNMLPMKELVAVLEALGARNV
                     NTYIQSGNVVFESPEKDPARLSSKMSQAIRKRRGFEPYILLLELEDIERAIRKNPFPE
                     AASDPKALHAGFLASKPETPDLEALENLKSGSERFRLIDRVFYLHAPDGIGRSKLAAK
                     AEKLLGVPMTVRNWRTVCTIRDMART"
     gene            2579..5308
                     /locus_tag="AC16CY_20295"
     CDS             2579..5308
                     /locus_tag="AC16CY_20295"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3356204.1"
                     /translation="MTLPVGSRIGPYEVKGLLGAGGMGEVYRARDTTLARDVALKVLP
                     DVFALDPDRLARFRREAQVLASLNHPHIAAIYGLEESHSSTGSGRAAVQALVLELVEG
                     PTLADLIAGASKDAPLPVRRGHAIPLEEALRIARQIAEALEAAHEQGIVHRDLKPANV
                     KVRADGTVKVLDFGLAKAFDPAATASDRSQSPTLTSPAATRLGVILGTAAYMSPEQAR
                     GKVVDKRTDVWAFGCVLYEMLLGTRPFDGDDVSETMARVIEREPDWNAFAAVVPPSIA
                     RLVRRCLQKDPQNRLRDIGDARLELRDALAEAGQETSGEPALVARRSFGSLAAVLAAG
                     LILGSVGAWAFTYVRSGGTPAATSTAGPPLVAEINLPADAALALDSEAANVGYDSTLL
                     DLSPDGRTLVYVGSSGGTVRLFARQLDSFDIQPLAGTEGALHPFFSPDGRSVGFLTND
                     KVKTYSFTTGTTTTICDVDVGVVGTWTADDQLFFGAGEGRQLFRVSAHGGTPVMVADA
                     REGFRYGRVTPDGRHVLVTYRRLGIGADFAQIRLMNLATKETKTLTTDGYDARLTSAG
                     DLVFGRSGRVFAARFDPERQVVGDPVPIASDVRMHALYPHLQLAVSTTGVLAYVPGGD
                     LAVAGLAWVNRQEQAEFLPLEPRVYGMFDLSDDGRRLAIQVGDTQDYILVYDIDRGSS
                     RRLPTTDSAGWPKWSPTGDALAYTSFSEGKPYRVLVQRVDSDRPPVAVAESQTRLTPS
                     TWNPDGRLTFYEFPTNRIGIVSVPRDTATSPAAPQYLDFAGSAHDISADGRWLAYSDA
                     SAGLSVRSLPVGERVHKIAEAGSEPRWCRTCDELIYRSGNRWFSIEVRRGPVFEWKQP
                     RLILQTQFNDSPGPSWALSPDGQRILVVKRKEDLPRTRLHVVHGWRAQAADGR"
     gene            5343..5852
                     /locus_tag="AC16CY_20300"
     CDS             5343..5852
                     /locus_tag="AC16CY_20300"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3356205.1"
                     /translation="MTPDDVRTLIDYNYWARDRVLDAVQPLTPEQFTRDMGNSFRSVR
                     DTLAHMYAAEWIWYSRLIGESPTALPPADRFPDLASLRSEWRDLEGKLRAFFDGLDER
                     GIERVIEYRSAMFGPGASVLWQMLQHVVNHGSYHRGQVTTLLRQLGATPPKSTDLIGF
                     YRERAAART"
     gene            complement(5857..6729)
                     /locus_tag="AC16CY_20305"
     CDS             complement(5857..6729)
                     /locus_tag="AC16CY_20305"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021982.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CE1 family esterase"
                     /protein_id="HIR3356206.1"
                     /translation="MRARHVVTAAFVVLGATAAHGQGRPAGPASCDLPHQPGVTSQQL
                     MSGQRQRAYRLFVPPGYDGRQRLPLVLDLHGSGGNAAGQARNSGLETLSASESFIVAT
                     LEADGGRWNVPVQENRPDDVAYVRDVMGHVGARVCLDETRVYATGFSGGGRMSSLLGC
                     RLGSRLAAIAPVSGLRAPGVCDGRPVPVLTFHGLADPQNTYDGHAANRGAEWLESVPD
                     ALAGWARLNSCKGDAILDDPPGPLSTLRYEGCAAGTEVRMIRIDGLGHTWTKKEVDTT
                     GVMWQFFKSHRLKN"
     gene            6823..7221
                     /locus_tag="AC16CY_20310"
     CDS             6823..7221
                     /locus_tag="AC16CY_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3356207.1"
                     /translation="MAIRIVRLGSPRALGEGLRLGTVRRPPRGVSKNELASRDFYDVW
                     VPGVAPSELLLKQGKQSVDSEARWKAFARRYRSEMKRPEAARWLDLLAALSHTADLSV
                     GCYCEDESRCHRSLLRQLLEERGARLTPSR"
     gene            complement(7205..7627)
                     /locus_tag="AC16CY_20315"
     CDS             complement(7205..7627)
                     /locus_tag="AC16CY_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3356208.1"
                     /translation="MTTVVLDRPVPKAWPIALWVVQFLLAFLFGGAGFAKLTTPLPEL
                     ATMLPYTADLPGALVRFIGASELAAAVGLVLPALFRVLPFLTIWAAIGLNIIMWLATL
                     FHLFRAEWRDMLVTFVIGNVAAFVALGRLKKAPISARG"
     gene            complement(7630..8643)
                     /locus_tag="AC16CY_20320"
     CDS             complement(7630..8643)
                     /locus_tag="AC16CY_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356209.1"
                     /translation="MGCRIHWGRAVAIVSVALAGEVYVAAPALAQSAAQTASRVAPAA
                     SWNHPRTPWGDPDIQGTWTTDDTIGVPVERPQALGNKLFLTDEDLAERARQQEAARRG
                     AGGGGGVGPPDHWGESASKPVRQTSFVVDPPNGRIPDVTLEAQKRAAGRDRGSFGTGP
                     FNRPEDLTNFDRCISRGVVGSLLPRPYGNGLDIVQAPGVVAIRYEMIHEPRIIRLGDA
                     PHVGQSIRQYMGDARGHWEGDTLVVVTRNFTDRMSIGFNGNGLRHSEAMTLVERFTRV
                     DTGTIQWEVTVDDPKTYTRPFKIATPIRLQNDYQVYEYACHEGNYGMRNILSAARADE
                     QKQ"
     gene            8853..>9151
                     /locus_tag="AC16CY_20325"
     CDS             8853..>9151
                     /locus_tag="AC16CY_20325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3356210.1"
                     /translation="MPFRILLVEDDRASRQGLSALLSGAGYSVAETASMADALDIIQH
                     DPPDLIITDIRLQEFNGLYLAAVNPLRIPVVIVTGYPDPGLEQEARNLGADFLLKP"
BASE COUNT         1485 a         3052 c         3045 g         1569 t
ORIGIN      
        1 tcgggtccgc ccccctctcc agcaggaggc gcatcgtcgt gatgtcgccc gacatcgagg
       61 cccggatgaa cggcgtctgc ccgatcatgt tgatccaggt caggtcgccg agcgaggtga
      121 cccaccgtcg aatcggaggg atctctttcg tcctggcgtt cgggttggcg ccccggtcga
      181 ggagcagctt caccatctcg agcgccgcag cgcgatcgat cccgtgctcg ttgccccgcg
      241 tgaaatcgcg gttgcggatc tcgacggccg agaagagcgg cgtccgcccc cagaagtcgg
      301 cggcgttgac atcggcgccg cgctcgacga gcaggcgggc cacgtccatg tggtcgttcg
      361 tgatcgccat gagcagcggc gagatggcgt tggcttcggc gaggttgacg tctgccctgg
      421 cgtcgagcag cagccgcgcc agcggcagat gcccgtcgcg cgcggcgtag agcagcgctg
      481 tcatcgcgcc ggggaccgcc gcgcgcgagc cgcgctccgg ccagccgccg cgaatgatgc
      541 cgagcccgtg cgaaccgccg ccggcattgg gcggcgtcca ttgcggcgtc gcgcccacgc
      601 gcgtggcggc gttcacgtcg gcaccgtgct cgatgaggag cttcgccgcc gctgcctgat
      661 tcgagcggac ggcccacatg agcggcgtct gcttcgtcgc cgtgtcggcg gcgttcaccg
      721 atgcgccgcg ctcgacgagc gatgccaccg agtcgaggtt gccggctcgc accgccatga
      781 cgagcagcgt ctcgccgctc gcatctctcg cgtccggatc ggcgccagcc cgcaggagac
      841 gcgcgaggat ggcgctgttg ccgttggacg tggcgacgtg cagcggcgtc acgccgaacc
      901 ggtttgcgag cgtggcgttc gcgccggcct cgagcaggag gttcacggtc tcgagatcgt
      961 cggcctgcac cgcccagtga agggccgtgg acccgtcggc cagcggcgcg ttgacctcgg
     1021 ccttctgcgc cagcagcgtg cgaatggcag cgcggtcctc gcggcgcgcc gcctcggcga
     1081 gtcgagcgtc gctctcggcg gccgagctga tggaagcgta aacaagcagg ctcgacccga
     1141 cgtaagccaa gacacgagcg gacattgtgg tccgaagcct aacagattcc ggtcctggat
     1201 atgaacacga cagcacggtt tggacgcgtc gtcgcactcg gcgctatcgc atggatggcc
     1261 ctgtgtttcc ccgcgcctct gcagtcccag gcgcccacgg gctcgacacg tatcgagatc
     1321 acggcaggct cgaccgcgga gtaccgcgtc cgtgaacagc tcgctcggct gaacttcccg
     1381 aacgacgccg tcggcagaac cgactcactg acgggcgcca tcgtcgtcgg ccccggcggc
     1441 gcgatcgcgc cgggatcgaa gctgacggtt gacctgcgcg atctgcagag cgacgccgat
     1501 cgccgcgaca acttcctgcg tcagaacacg ctgcacacga gcaagttccc gatggccgag
     1561 ttcgtcccgc ggcgacagga ggggctgccc tcgccgatcc cttcatcggg caaagcgggc
     1621 ttcaagctga ttggcgacat gaccgtccac ggtgtgacga cggaactggc ctgggacgtg
     1681 gcggctacgt tcgcgcccgg ggcggtctcc ggcgaggcga cgacgcgatt tcagttcgcg
     1741 aagttcggcc tggcgatccc caaggtgatg ggattgctca gcgtggacga cgacatcagg
     1801 ctggtgctga acttcaaggc ccgccgagtg gaggttcccg ccgtcgcgtc ctcctcgaac
     1861 tagtcaaggg ctccgcccgt gctatcccgg cgaggctcac gtccgcgcca tgtcgcggat
     1921 cgtgcacacg gttctccagt tgcgaaccgt catcggcaca ccgagcagtt tctccgcctt
     1981 ggcagccagc ttcgagcgac cgatcccgtc cggggcatgg aggtagaaca ctcgatcgat
     2041 gagccggaat cgttcgctgc cgctcttgag gttttcgagc gcctcgagat ccggcgtctc
     2101 cggtttggac gccaggaacc cggcgtgcaa cgccttcgga tcgcttgccg cttcgggaaa
     2161 cgggttcttc cgaatcgccc tttcgatgtc ttccagctcc agcagcagaa tgtagggctc
     2221 gaatccgcgg cgcttcctga tggcctggct catcttgctc gaaagccgtg ccgggtcttt
     2281 ctcggggctc tcgaatacga cgttgccgct ctggatgtag gtgttgacgt ttcgagcgcc
     2341 gagcgcttca agaacggcca cgagctcctt catcggcagc atgttcttcc cgccgacgtt
     2401 gatggcgcga aagagggcga tgtaggtctt caatttcggc gcctcggctc gcagcataac
     2461 gccaatgccg ccccgcgagg agtacactca cgcgctgaga catgacgttg acccggggcg
     2521 accggctcgg tccctacgag attctctcgg tgctcggtgc gggcccctcg actcgcctat
     2581 gacgttgccc gttggatcgc gaatcggtcc gtatgaggta aagggcttgc tcggggcggg
     2641 cggtatgggt gaggtctatc gagcccgcga cacgacattg gcccgggacg tggcgctgaa
     2701 ggtgctcccc gatgtcttcg cgctcgatcc cgatcgtctc gcgcgcttcc ggcgggaagc
     2761 gcaggtcctg gcttcgctca atcatcctca catcgccgcc atctacggcc tcgaggagtc
     2821 gcactcttcg acgggctcag ggcgggcggc ggtgcaggcg ctcgtcctgg aactggtcga
     2881 gggaccgacc ctcgcggacc tgatcgccgg cgcgtctaaa gacgcgccgc taccggtacg
     2941 tagaggacac gcgatccccc ttgaggaggc gctgcgaatt gcgcgacaga tagccgaggc
     3001 gctcgaagcc gcgcacgagc aggggatcgt tcatcgggac ctgaagccgg ccaacgtcaa
     3061 ggtgcgcgcc gatggcacgg tcaaagtgct cgacttcggc ctggcgaaag cgttcgatcc
     3121 cgccgcgacc gcatccgatc gctcgcagtc gcccaccctg accagtccgg ccgcgacgcg
     3181 gctcggcgtg atcctgggca ccgccgccta catgagcccc gagcaggcgc gcggcaaggt
     3241 ggtggacaaa cgcaccgacg tgtgggcgtt cggctgcgtg ctgtacgaga tgctgctggg
     3301 cacacgtccg ttcgacggcg acgacgtctc cgagaccatg gcgagagtca tcgagcggga
     3361 accggactgg aacgcgttcg cggcggttgt gccgccgtcg atcgctcgcc tcgtccggcg
     3421 ctgcctccag aaggatcccc agaaccgcct tcgcgacatc ggtgatgcgc ggctcgagtt
     3481 gcgcgacgcg ctggccgagg cgggccagga gacctcaggc gagcctgcgc tcgttgcgcg
     3541 acggtcgttt ggttcgctgg ccgcggtgct cgcggcaggg ctcattctgg gtagcgttgg
     3601 agcctgggcg ttcacgtacg tcaggtcagg aggtacgccg gccgcaacat cgaccgctgg
     3661 tccgccgctc gtcgcggaaa tcaacctgcc cgccgatgcg gctctcgccc tggactccga
     3721 agcggccaac gtcggatacg attccacgct gctcgatctc tcaccggacg ggcggacgct
     3781 cgtctacgtc ggatcgtcag gcgggactgt tcgcctcttc gcgcgtcagc tcgacagctt
     3841 cgatatccag ccgctcgcgg gcacggaagg cgcgctgcat ccattcttct cgccggacgg
     3901 gcggtcggtg ggctttctca ccaacgacaa ggtcaagacg tattcgttca cgacgggaac
     3961 gacgaccacg atctgcgacg tggatgtggg cgtggtcgga acctggaccg ccgacgatca
     4021 actgttcttc ggagctggag aaggccgcca gctctttcgt gtgagtgctc acggcggaac
     4081 accggtgatg gtggccgacg cgcgggaagg cttcaggtac ggacgcgtga cgcccgacgg
     4141 caggcacgtg ctcgtgacct accggcgcct gggcattggc gctgatttcg cgcagatccg
     4201 gctcatgaat ctcgcgacca aggaaacgaa gacgctcacg accgacggtt acgacgcccg
     4261 gctgacctcc gccggagatc ttgtctttgg ccgatcggga cgggtgtttg cggcgagatt
     4321 cgacccggag cgtcaggtcg tgggcgatcc tgtaccgatt gcgtcggatg tgcgcatgca
     4381 tgcgctctat cctcacctgc agctcgccgt gtcgacgacc ggcgtgctgg cctacgttcc
     4441 cggcggcgat ctggccgtcg ccggcctcgc gtgggtgaac cggcaggaac aggcggagtt
     4501 tctcccgctc gagccacgcg tctacggcat gttcgacctg tcggacgacg gccgccggct
     4561 ggccatccag gtaggcgaca cccaggacta catcctggtc tatgacatcg atcgcggctc
     4621 ttctcggcgt ctgccgacga ccgacagcgc tgggtggccg aaatggtcgc ccacgggaga
     4681 cgcacttgcg tacacgagtt tctcggaggg caagccctac cgcgtgctcg ttcaacgcgt
     4741 ggattcggac cggccgccgg ttgccgtcgc cgagtcacaa acgcggctca ctcccagtac
     4801 gtggaaccct gacggacgcc tcacgtttta cgaattcccg acaaaccgca tcggcatcgt
     4861 ctccgtcccg cgcgacaccg cgacctcgcc ggccgcgccg cagtacctgg atttcgcggg
     4921 gagcgctcac gacatctccg ctgacggccg ctggctggcc tactccgatg cgagtgccgg
     4981 cctcagcgtt cgctcgctgc ctgtcggcga acgcgttcac aagatcgcgg aggccggcag
     5041 tgaaccgcga tggtgccgca cgtgcgacga gctcatttac cgcagtggaa accggtggtt
     5101 cagcatcgag gtgcgacggg gaccggtgtt cgagtggaaa cagccgcggc tgatccttca
     5161 aacccagttc aacgacagcc cggggccgtc atgggcgttg tctccggacg ggcaacgcat
     5221 cctggtggtg aagaggaagg aagacctgcc acgaacgagg cttcacgtcg tccacgggtg
     5281 gcgtgcgcag gcggcagacg ggcggtgagc caggcgtgct caacaaacgg tagactgctc
     5341 gaatgactcc agacgatgta cggacgttga tcgactacaa ctactgggca cgcgaccgtg
     5401 tactggacgc cgtgcaaccg ctgacgccgg agcagttcac gcgggacatg ggcaacagct
     5461 tccgctcggt ccgcgacacc ctcgcgcaca tgtacgccgc cgagtggatc tggtactcgc
     5521 gcttgatcgg tgagtcgccg acggcgctcc ctcccgccga ccggtttccc gacctggcat
     5581 cgctccgcag tgaatggcgc gacctcgaag gcaagctccg cgcgttcttc gacgggctgg
     5641 atgagcgggg gatcgaacgc gtcatcgagt acaggtcggc catgttcgga cccggagcgt
     5701 cggtcctgtg gcagatgctc cagcacgtcg tcaaccacgg gtcgtaccac cgcggccagg
     5761 tgaccacgct gctgcggcag ctcggcgcga cgccgccgaa aagcaccgat ctgatcgggt
     5821 tctatcgaga gcgcgccgcg gcgaggacgt aatggctcag ttcttcagac gatgactctt
     5881 gaagaactgc cacatcacac ctgtggtgtc gacttccttt ttcgtccagg tgtgaccgag
     5941 gccatcgatg cgaatcatgc ggacttcagt acctgcagcg cacccctcgt accgcagggt
     6001 ggacagcggg cctggaggat cgtcgaggat cgcgtcccct ttgcaggagt tgaggcgcgc
     6061 ccagcccgcg agcgcatcgg gaacgctttc cagccactcc gcgcctcggt tcgctgcgtg
     6121 gccatcgtag gtgttctgag ggtcggccag gccgtggaac gtcagaacag gaaccggacg
     6181 gccgtcgcac acgccgggag cccgaagccc ggagaccggc gcgattgccg cgaggcgcga
     6241 gccaagcctg cacccgagca gcgacgacat ccgcccgccg cctgagaatc ccgtcgcata
     6301 gacgcgcgtc tcgtcgaggc acacacgcgc gcccacatga cccattacat cgcggacgta
     6361 ggccacgtcg tctggacggt tctcctggac gggaacattc cagcggccgc catcggcttc
     6421 gagcgtcgcc acgatgaagg actcgctggc gctcaatgtc tcgaggccgc tgttcctggc
     6481 ctggcccgcg gcgtttccgc cgctcccgtg cagatcgagc acgagtggca ggcgttgccg
     6541 accatcgtat ccagggggga cgaacaaccg gtacgcgcgc tgccgctgcc ccgacatcag
     6601 ctgttgcgac gtcacgcccg gctggtgagg caggtcacag gacgcgggcc ccgcagggcg
     6661 accctgccca tgcgcggccg tcgcgccgag cacaacgaag gctgccgtga cgacgtgtcg
     6721 tgcgcgcatc ttcatctctc cttcgccagc gctgcctgcg ccaaccgtcg tgattataga
     6781 ggcgggccac cctccgcgct cacgggcgtg atatgaagac gcatggccat acggatcgtc
     6841 cggctcggga gcccgcgcgc actgggagaa gggctgcgcc tcggtactgt tcgacggccc
     6901 ccgcgcggcg tgtcaaaaaa cgagctggcg tcgcgcgatt tctatgacgt gtgggtgccc
     6961 ggtgtggcgc cgagcgagct gctcctgaaa caagggaagc agtcagtcga cagcgaggcg
     7021 cgctggaaag cgtttgcgcg gcgctatcgg agcgagatga agcggccgga ggcggcacgg
     7081 tggctggacc tgctcgccgc gctctcacat actgctgatc tgtcggtcgg atgctactgc
     7141 gaggacgaaa gccgctgcca tcgctctctg ctgcggcaac tgctcgagga gcgcggcgcc
     7201 aggctcaccc cctcgcgctg atcggcgcct tcttcagccg gcccaacgcc acgaaggccg
     7261 cgacgttccc gatcacgaat gtcacgagca tgtcgcgcca ctctgccctg aacaggtgga
     7321 acaacgtcgc gagccacatg atgatgttga gtccgatggc cgcccagatc gtgaggaacg
     7381 gaaggactcg aaagagcgcc gggagcacga gcccgacggc ggcggcgagc tccgacgccc
     7441 cgatgaagcg aacgagcgcg cctggaaggt cggccgtgta gggcagcatc gtcgccagct
     7501 cgggcagcgg cgtcgtcagc ttcgcgaagc ccgccccgcc gaagaggaac gccaggagaa
     7561 actgcacgac ccagagcgcg atgggccagg ctttggggac cggtcgatcg agcacgaccg
     7621 tcgtcatgtc tattgcttct gttcgtcggc gcgagcggcg ctcaggatgt tccgcatgcc
     7681 gtagttgccc tcgtggcacg cgtactcgta cacctgatag tcgttctgga gccgaatcgg
     7741 cgtcgcgatc ttgaagggcc tcgtgtaggt cttcgggtcg tcaacggtga cttcccactg
     7801 aatcgttccc gtgtccacgc gcgtgaagcg ctcgacgagc gtcatcgcct cgctgtggcg
     7861 gaggccgttt ccgttgaagc cgatgctcat gcggtccgtg aagttcctgg tcacgaccac
     7921 cagcgtgtcg ccctcccagt gcccgcgcgc gtcgcccatg tactggcgga tgctctggcc
     7981 gacgtgcggg gcgtcgccaa gccggatgat tcgcggttcg tgaatcatct cgtatcggat
     8041 cgcgacgacg ccgggcgcct gcacgatgtc gaggccgttg ccgtacggcc tggggaggag
     8101 cgatccgacc acgccgcgtg agatacaccg atcgaagttg gtgagatcct ccggccgatt
     8161 gaacggtccc gtgccgaagc ttccgcggtc gcgcccggcc gcgcgcttct gggcttcgag
     8221 tgtgacgtcg ggaatccggc cgtttggcgg atcgaccacg aacgaggtct gacgaacggg
     8281 cttggacgcg ctctcgcccc agtgatcggg cggcccgact cccccgcctc cgccggcccc
     8341 acgacgcgcc gcttcctgct gccgcgcgcg ctcggccaga tcttcatcgg tcaggaacag
     8401 cttgttgccg agcgcctgcg ggcgctccac cgggacgccg atcgtgtcgt ccgtcgtcca
     8461 ggtgccctgg atgtccggat cgccccaggg cgtgcgcgga tggttccacg acgcggccgg
     8521 cgccacacgg gatgcggtct gcgcggcaga ctgcgctagc gcaggcgcgg cgacgtacac
     8581 ctctcccgcg agggcaacag acactatcgc aacagcccga ccccagtgga ttcggcagcc
     8641 cataccgtcc ctcccagcgt ggccggatcg tagcacgcgg ggcccagaag cccggtcctt
     8701 tagtcggtga gccctgggat tgcggatcac aacctgggct cgaccgtttt cacattcgta
     8761 tgaagccatg acactttctg acgattcggc ccttcaaaca acgggaaatc cgacctgcca
     8821 gttggtctgt cccttgcgtg tggaaggggg gcatgccgtt ccggattctc ctcgtcgagg
     8881 atgacagggc ctccaggcag ggcctgtcgg cgctcctgtc cggggcgggc tacagcgttg
     8941 ccgagacggc gtccatggcc gacgcgctcg acatcatcca gcatgatccg ccggacctga
     9001 tcatcaccga catccggctc caggagttca acggcctgta cctggcggcg gtgaatccgc
     9061 tccgcatccc ggtcgtgatc gtcaccggat acccggaccc cggcctcgag caggaagcgc
     9121 gtaatcttgg cgccgatttc ctgctcaaac c
//
LOCUS       DBNGSH010000884        13459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3844964, whole genome shotgun sequence.
ACCESSION   DBNGSH010000884 DBNGSH010000000
VERSION     DBNGSH010000884.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 13459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13459
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3844964"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="AC16CY_20330"
     CDS             complement(<1..1269)
                     /locus_tag="AC16CY_20330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3356211.1"
                     /translation="MNDGLGWALEHYVIVGPIASGRFGSVFHARHVATNQAVALKLIP
                     RQGPESDEKVAAERHGAILQQHFSLAHKNLVPDVFEHGPMGGFYAIAMEFVAGQQLTE
                     LVKSGPLPPQRAGDIGLAVCTFLEKAHQFKTTIEDVHYELIVHADLKPDHILILPDDT
                     IRVLDFGIAKALETRSLVTTNKWGSVQYASPERLQSDGRVNRHTDFWSLGVMLFEMLA
                     GYRPYRRFEYSVSRLDRAIRTMEAPEGLPIGSDVVLGAVVRKLLATQIERRYASAAAI
                     AKDLEAYRRGEPVAAALEEARAGQETIRVSGNPLSAPGSGLPATPRPRPARSLWSMRR
                     IGRVASISALVVVALLEGIGLVRGAQARARVGAFEPTDVRAARAAYQAVGRWTPLGLG
                     SRSVAGALTARMVDLADRAILDYRTELPTVN"
     gene            complement(1283..2860)
                     /locus_tag="AC16CY_20335"
     CDS             complement(1283..2860)
                     /locus_tag="AC16CY_20335"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF012693.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3356212.1"
                     /translation="MIEPVAALSATGLRAAGASDAGRVRRNNEDRFYFDLERGIFLVV
                     DGVGGHAAGEVAASIATDVIVDRLERGGASPASVMRQAITCANNEIHRQARRSVQHFG
                     MTCVVTVALVSDDRLTIGHVGDSRLYKVTPDGLWKLTRDHSPVGEQEDAFEISEVEAM
                     RHPRRNEVFRDIGSAPRKADDPEFIETVETRLEPDSALLLCTDGLSDMLPSTSLERII
                     RCHAGDPQKVVDELIGAANDAGGKDNVTAVYIEGRAFSGAALPSAARPDRMSSGQIRP
                     AGMSRRRILWLGIGAVFGGALGLAATASVDRVVTMSRGRVLAAGGDGAFPSIAAAMSA
                     ASPRDVVRVEPGEYAEAVVIKKGVDLIARVAGSVVLVAPTGAENWRALTASGENHVSG
                     IRVLGRPDAPIATGVTLDGGDLVLDDSGFEGNIDVAVDVAHDGDIVVRANRFSDVRGL
                     PLRIGRSARPAVRQNIFTRGTEEHGPAVHVLEDAMPTLDDNLFVGYAEPIGAPPARRQ
                     QLLPGNFVTRGQERVGR"
     gene            complement(2939..3835)
                     /locus_tag="AC16CY_20340"
     CDS             complement(2939..3835)
                     /locus_tag="AC16CY_20340"
                     /inference="COORDINATES: protein motif:HMM:NF023818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HIR3356213.1"
                     /translation="MKVASAPTGRKIVQELEREMEDRLYPLFYRTLPPAEYHVYLHPT
                     DYRQIESIVPLIVTDAQQRLSDRVDRLNGRGRWSLVSEKPSRIEIPAGGWAVFIQPDP
                     NDEVAAGELGIVSRLAIPAPAAFDGGAPTVRIGRTVVTETIRRSAATEVTAPGSTTAS
                     AGIAQAPALSPGADPGASSGFATLTYVDDEGPHLFTMRKASIAIGRGGDAHWVDVQVT
                     SSTRISREHCRIRRDGSGEFFLEDVSTWGTSVNGTRVAPYGRSDMSNSGEPAQGFRLP
                     RQARIELADALTIEFSAHAEPC"
     gene            3923..4603
                     /locus_tag="AC16CY_20345"
     CDS             3923..4603
                     /locus_tag="AC16CY_20345"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3356214.1"
                     /translation="MPIQFGEFTLDSAGRQLFRGSEAIHLSPKAFDVLSVLIERRPTA
                     VSKTDLHELVWAGTFVADASLTMVITEVRKALGDEAQHPRFIRTVHRFGYAFIADGHS
                     VRVEPDRDVRSVRLQPDPPGAWLVWNERALVLRDGQNTVGRDPQCEVWVDAAGVSRRH
                     ARVTVIRGAATLEDLGSKNGTMLDGASIRTVMPLSDGDVIQFGSVEMQFRLGSDRNAT
                     ETVRLDRA"
     gene            4634..7123
                     /locus_tag="AC16CY_20350"
     CDS             4634..7123
                     /locus_tag="AC16CY_20350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR3356215.1"
                     /translation="MHAGALRTLEFDRIVSLVSGLAVTPTGRDRLAALHPATDASLIV
                     AAQRATSEGTRFLASHPGFPLRAPSDLESILEALALDGRALEPMRLLGLSGYLESIES
                     SRGAIVKVGAAFPILSRQVEGVASFSDEIADVRRKIEPSGDIADHASPALSQIRDRLR
                     RQKQRLRSTLDSLLRGRDTSKYLQEQVVTDRNGRYVLTVRAEHRSAIPGIVHGGSTSG
                     ASLFVEPLETVEINNDIVALEEQEAEEIRRILLALTDAFRARPAELARTIEVATELDV
                     IQARARLSLLTSSVEPIVAADGTFELRGARHPLLVLRGKWGRESFFEDAAPGDAMKND
                     SRPHFNSVVPVDILLSPPTRILVITGPNTGGKTVALKTAGLLAAMAQSGLHVPAEPGS
                     RIPIFKSLFADIGDEQSISASLSTFSAHVTNLVSMDRDLALPALVLLDEVGAGTDPVE
                     GGALGIAVIDHFRRRGAHLVATTHYDSLKSYASTTDGVAGAAFGFNPETFAPTYRLLY
                     GSPGRSLAIEIAARLGMPPSVIAAARENLSDREKQLAEHLARIDDEMRRLEAEHRAVA
                     RERATVSETERHLRAREESVRERENTYKRRLDAKLDEQLRLGRREIDTIIEGLKAKTA
                     ELSEQAERRIRLNTGDAGAVKADARAAIERVVSRLKTGPDLPESASHPSARDHRARIE
                     PGMRVVVGALGFEGVVVEIHGKQAEIDVKGKRLRAAVQDLRLAGAGGGAPARPSVRVN
                     VDLQPRQGSLSEVNVIGCTVDEAIARVEKFLDDSTVSDQQIVRIIHGHGTGQLRRGLA
                     GFLKEHPLVAKFQAAPTDQGGSGVTVVELKD"
     gene            7167..8936
                     /gene="dnaG"
                     /locus_tag="AC16CY_20355"
     CDS             7167..8936
                     /gene="dnaG"
                     /locus_tag="AC16CY_20355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR3356216.1"
                     /translation="MALYPQSFIEDLKHHADIVVVIQDYVSLKKAGTSYKGLCPFHGE
                     KTPSFHVNRDKGFFHCFGCGVGGDVFKFLELHEKVGFQDAVKQLAQRFGLTLPELEQS
                     DEQRASAAERESLLKIHEAAAAWFREQLLSPAGVRIRRQIAGRGVTDATSEAMGLGFA
                     PPSRDALMQRLTTQGFSDGLLIRSGLVTRRDDGSIVDRFRNRLIIPIRRDTGSVIAFG
                     GRAVDADQQPKYLNSPETPIYTKGRTLYGLDLSKAAIRERGFAVLVEGYFDFGQVHQA
                     GFPAVASCGTALTPQQVQQLRRFTSRVVLSFDPDAAGQGATEKSCELFVAEGFDVNVA
                     VLPSGQDPDAYIRSNGPAGYGERLKQSKSYLEYLLDRASAGHDLNSDEGRVRFLAAML
                     PTAARIPDAAMRDRFADRLAFKANVTDEVVRAKIREAAVQRQTTISANALPSVGRVTK
                     AEKGLIWWLVHDPGPALTALDELNPSDFEALAAGSVLDLARKLNENRGFSPSVLLERL
                     NMVEAQVVTAIASEPEPPALSLSFCVRELRRGRYERERAAVQREIAELQTRGAASGVE
                     LDALLVRKGDLGRLIQALVLSED"
     gene            8939..10639
                     /gene="rpoD"
                     /locus_tag="AC16CY_20360"
     CDS             8939..10639
                     /gene="rpoD"
                     /locus_tag="AC16CY_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR3356217.1"
                     /translation="MSIEEKYDEVRQLITVGKEKGYLLYDEVNELLPSEITSSEELDD
                     LFNAFGSAGIEVVDSDKAYRGDKDFDREGSGDEPELDLTPGALDKTNDPVRMYLREMG
                     TVPLLTREGEVAIAKRIERGKLAVIKSISRTPTIAKRILIMGDQLRAGERTIRELVIF
                     SDEEITDEVIQERAVEVLGQIEAVRKARGNYLKLGEKLDEVTKKDKRRFRRARLKTMR
                     AWVEVSRRIRDIEFTESIKRRLVDEVKDAVDAVQKVQREAEHLDKQISPRVKLKGPRL
                     REEEKKALQKRLKDIRVQVKQMIDDLEETPERLRRTIEVIQRGEAQAEQAKKELVEAN
                     LRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITR
                     AIADQARTIRIPVHMIETINKLIRTSRALVQELGREPTSEEIAKRMDIPVAKVRKVLK
                     IAQEPISLETPIGEEEDSHLGDFIEDRNIVSPAEAVINLNLKEQTEAVLKTLTPREEK
                     VIKMRFGVGDGSEHTLEEVGQNFAVTRERIRQIEAKALRKLRHPSRSRKLKAFLEGRT
                     "
     gene            10689..10761
                     /locus_tag="AC16CY_20365"
     tRNA            10689..10761
                     /locus_tag="AC16CY_20365"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10722..10724,aa:Ile,seq:cat)
     gene            10778..11521
                     /locus_tag="AC16CY_20370"
     CDS             10778..11521
                     /locus_tag="AC16CY_20370"
                     /inference="COORDINATES: protein motif:HMM:NF049746.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3356218.1"
                     /translation="MSPDLERLIRLQHLEAAIAEARAAIALHPQRLAALDARLEEARR
                     AVDDVKQRLKTNHDARLELDKGIALYQGRLTKYKDQLSEVKTNREYQAMQHEIAAAQN
                     DLGAVEEKVLVRMMEGDDLTATAKRAEAALAAATKVVEAEKKTLAEELASTESSLAKA
                     TEGRAALAREMEPRLVALFEQVARVRKGVAICVATKDGLCSLCHVRLRPSVFQQVRHN
                     DTILQCESCQRVLYWIPPPSPVSPPVVHV"
     gene            11577..12020
                     /locus_tag="AC16CY_20375"
     CDS             11577..12020
                     /locus_tag="AC16CY_20375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024848.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HIR3356219.1"
                     /translation="MTQPSLFGLETGGSAVANIDGGSRGNPGPAGYGVRIEREDGTLV
                     DLKQSLSACTNNVAEYSGLLAALRWAADHGVTTLRVRSDSELLVKQMKGEYRVKNPGL
                     QPLYEEARALARRIGRVTFEHVRREFNKDADRLANEAMDEAETSP"
     gene            complement(12035..12553)
                     /locus_tag="AC16CY_20380"
     CDS             complement(12035..12553)
                     /locus_tag="AC16CY_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB-like translocation protein"
                     /protein_id="HIR3356220.1"
                     /translation="MSYRAIVRDDFGWQPPVNAGSGINSPGNEQNPNFFENDDVGVPQ
                     LFFSNATDGIYVSDLLPDGTFGPARPVVELDSTALDRSPSVRFDGLEVFFQSNRPGSF
                     GLLNLWTATRDTVSDAWSEPTNLGPLVNSLAIDGDPEITSDRETLYFVSNRTGGVGGF
                     DLYVTTRAKHTK"
     gene            13168..>13459
                     /locus_tag="AC16CY_20385"
     CDS             13168..>13459
                     /locus_tag="AC16CY_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3356221.1"
                     /translation="MRTASTGQVTGLLRAWAAGHPEAQDQLLPLVYQELRRRAAGHLR
                     RERKDHTLQPTALVHEAYLRLLGQRADWQSRTQFFALASRMMRRILVDHARAR"
BASE COUNT         2396 a         4388 c         4543 g         2132 t
ORIGIN      
        1 gttgaccgtc ggcagttcgg tcctgtagtc gagaatcgcg cggtccgcca gatcgaccat
       61 ccgcgccgtg agcgctccgg ccacactgcg tgaaccgagg ccgagcggcg tccacctgcc
      121 gacggcctgg tacgccgctc gcgcagccct gacgtcggtc ggctcgaagg ctccgacgcg
      181 cgcgcgcgcc tgcgccccgc gtacgagccc gatcccctcg agcaaggcga cgacgacgag
      241 ggccgaaatc gacgcgactc gaccgatccg ccgcattgac cagagggacc gagcaggtcg
      301 cgggcgcgga gtcgccggga gcccggagcc cggagccgag agcgggttgc cagaaacgcg
      361 aatcgtctcc tgccctgcgc gcgcctcctc gagagcagca gcgaccggct cgccgcgtct
      421 gtacgcctcg aggtccttgg cgatggccgc cgcggaggcg tagcgccgct cgatctgcgt
      481 cgcgagcagt ttcctgacga cggccccgag taccacgtcg ctgccgatgg gcagcccctc
      541 gggcgcttcc atggtccgga tcgcgcgatc gaggcggctg acgctgtact cgaagcgccg
      601 atacggccga tacccggcca gcatctcgaa cagcatcacg ccgagcgacc agaagtcggt
      661 gtgccggttg acgcgcccat ccgattgcag ccgctcgggc gacgcgtact gaacggaacc
      721 ccacttgttg gtcgtgacca ggctgcgcgt ctcgagcgcc ttcgcgatgc cgaaatcgag
      781 gacgcggatc gtgtcgtccg gaaggatgag gatgtggtcg ggcttcagat cggcgtgcac
      841 gatgagctcg tagtgcacgt cctcgatcgt tgtcttgaac tggtgcgcct tttccagaaa
      901 cgtgcagacg gcgaggccga tgtctccggc gcgctgcgga ggaagcggac ccgacttgac
      961 cagctcggtg agctgttgtc cggccacgaa ctccatcgcg atcgcgtaga agccgcccat
     1021 cggcccgtgc tcgaacacgt cgggaaccag gttcttgtgc gcgagcgaga agtgctgctg
     1081 aagaatggcg ccgtgccgct cggccgcgac cttttcatcg ctctcgggcc cctgcctggg
     1141 aatcagcttc aacgcgacgg cctgattcgt cgcaacgtgc cgcgcgtgga agaccgaccc
     1201 gaatcggccg ctcgcaatcg ggccgacgat gacgtagtgc tcgagcgccc agccgagacc
     1261 gtcgttcacc ggcggctccg tgctagcgac cgacacgttc ctggcctcga gtgacgaagt
     1321 tgccgggaag caactgctgg cggcgcgcgg gcggcgcgcc gatcggctcg gcgtatccga
     1381 cgaacaggtt gtcgtcgagc gtcggcatcg cgtcctcgag gacgtgcacg gcgggcccgt
     1441 gctcctccgt ccctcgcgtg aagatgttct ggcgaacggc cggccttgcc gatcgtccga
     1501 tccgaagcgg cagccccctg acgtcggaga agcggttcgc gcggaccacg atgtcgccgt
     1561 cgtgtgcgac gtcaacggca acgtcgatgt tcccttcgaa tccggaatcg tccagcacga
     1621 gatcgccccc atcgagcgtc acgccggtcg caatcggcgc atcgggacgg cccagcacgc
     1681 gaatgccgct gacatggttt tcgccgctcg cggtcagcgc cctccagttc tcggcgcctg
     1741 tcggagccac gaggacgacc gaacccgcca cacgagcgat caggtccacg cctttcttga
     1801 tcacgactgc ctcggcgtat tcgcccggct ccacacgcac gacgtcgcgc ggggacgctg
     1861 ccgacatcgc cgcggcaatc gacgggaacg cgccgtcgcc gccggccgcg agcacgcggc
     1921 cccgcgacat cgtgacgacg cgatcgaccg acgccgttgc cgcgaggcca agggcgccgc
     1981 caaacacggc gccgatcccc agccacagga tccgacgtcg ggacatgccg gccggccgga
     2041 tctgcccgga cgacatccgg tcgggccgcg ccgcggaagg cagagccgcc cccgagaacg
     2101 cacggccttc gatgtagacc gcggtgacgt tgtctttgcc gcctgcatcg ttggccgcgc
     2161 cgatcagctc atcgacgacc ttctgcggat cgccggcatg gcaccggatg attcgctcga
     2221 ggctcgtgga cgggagcatg tcgctcaggc cgtcggtgca caggaggagc gccgagtcgg
     2281 gctcgaggcg tgtttccacc gtttcgatga actccggatc gtcggccttg cgcggagcgc
     2341 tgccgatgtc gcggaacact tcgttccggc gcggatgacg catcgcctcg acttcggaaa
     2401 tctcgaacgc atcctcctgc tcgccgacag gcgaatggtc gcgcgtgagc ttccagaggc
     2461 cgtccggcgt gaccttgtac aagcggctgt ccccgacgtg accgatcgtc aggcgatcgt
     2521 ccgagaccag cgccacggtg acgacgcacg tcatcccgaa gtgctgaacc gagcgccggg
     2581 cctggcggtg gatttcgttg ttggcgcacg taatggcctg gcgcatcacc gacgcgggcg
     2641 acgcgccgcc gcgttcgaga cgatcgacga tgacgtccgt ggcgatcgaa gcggccactt
     2701 cgcccgccgc gtggccgccc acgccgtcca cgaccaggaa gatgccgcgc tcgaggtcga
     2761 agtagaaccg atcttcgttg ttccgccgga cgcgccccgc gtcggaggcg ccggccgcgc
     2821 gcagacccgt cgcactgagc gccgcgacgg gctcaatcat ggctgctcac ccacgccagg
     2881 taggtcagcc agatgagggc ggcccccagc cccacgagaa acagcgcctg ggcggcaatc
     2941 agcacggctc tgcatgggcg ctgaactcga tcgtcagcgc gtcggccagt tcgatccgcg
     3001 cctgcctcgg cagcctgaag ccctgggccg gctctccgga gttcgacatg tcgctgcgcc
     3061 cgtacggcgc gacgcgcgtg ccgttcaccg aggtgcccca ggtactcacg tcctcgagga
     3121 agaattcgcc gctgccgtcg cgtcgaatgc ggcagtgctc gcgcgagatg cgtgtgctcg
     3181 acgtgacctg cacgtccacc cagtgggcgt cgcctccgcg gccgatggcg atcgacgcct
     3241 tgcgcatcgt gaacaggtgc gggccctcat cgtccacgta ggtgagcgtc gcgaagcctg
     3301 aagacgcgcc gggatcagcg cctggagaaa gcgccggcgc ctgcgcgatc cctgctgaag
     3361 ctgtcgtgct tcccggcgcc gtgacctccg tcgccgcgct tcggcggatg gtctcggtca
     3421 cgaccgtacg gccgattcga acagtcgggg ccccgccgtc gaatgccgcg ggtgcgggaa
     3481 tcgccaggcg cgaaacgatg ccaagctcgc ccgcggcgac ctcgtcgttc ggatcgggct
     3541 gaatgaagac ggcccagccg cccgccggga tttcgatccg ggaaggcttc tcggagacga
     3601 gcgaccagcg gccccggccg ttcagccggt ccacgcggtc gctgaggcgc tgctgggcgt
     3661 cggtgacgat cagcggcacg atcgactcga tctggcggta gtcggtcgga tgaaggtaga
     3721 cgtggtactc cgccggaggc agggtccggt agaacaaggg atagagccgg tcctccatct
     3781 cccgctccag ttcctgaacg atcttgcgcc cggtcggcgc gctcgccact ttcatacttc
     3841 accaggacga gtgcactgtc gcgcaggttc tggtgtaaag tcctcaagaa tccgcaagat
     3901 ttttccaaag cctcgcatcc ccgtgcccat ccagttcggc gagttcacgc tcgattcggc
     3961 gggtcgtcag ctgtttcggg gatccgaggc tattcatctg tcgcccaagg ccttcgacgt
     4021 cctgagcgtc ctgatcgagc gccgcccgac agcggtcagc aagacggacc tgcatgagct
     4081 ggtctgggcc ggaacgttcg tcgccgatgc cagcctgacg atggtcatca ctgaagtacg
     4141 gaaggcgctc ggggacgagg ctcagcaccc gcgcttcatc agaacggtgc accgattcgg
     4201 ctacgccttc atcgccgacg gacatagtgt ccgggtcgag ccggaccgtg acgtacgtag
     4261 tgtccggctt cagccggacc ccccgggcgc gtggctcgtc tggaacgagc gggccctcgt
     4321 gctgcgcgac gggcagaaca ccgtgggacg cgatccgcag tgtgaggtgt gggtggacgc
     4381 cgcgggcgtc tcccggcgcc acgcccgcgt caccgtcatc cggggcgctg ccacgctcga
     4441 agaccttggc agcaagaacg ggacgatgct cgacggtgcg tcgatccgga ccgtgatgcc
     4501 gttgagcgac ggggacgtga tccagttcgg ctctgtcgaa atgcagtttc gattggggtc
     4561 ggaccgcaat gcgaccgaga cggtccggct cgaccgagcg taagtggtac actcctccac
     4621 tcgagctccg tcgatgcatg cgggcgccct ccgaacgctc gagttcgatc gcatcgtgtc
     4681 cctggtctcc ggcctcgcgg tgacgccaac cggccgggat cgtctcgcgg cgctgcaccc
     4741 ggcgaccgat gcctcgctga tcgtcgcggc gcagcgcgcc accagcgagg ggacacggtt
     4801 tctcgccagt catccaggct tcccccttcg cgctccatcc gacctcgagt cgattctcga
     4861 agcgctggcg ctcgacgggc gcgcgctcga gccgatgcgg ctgctggggc tgtccggcta
     4921 cctcgagtcg atcgagtcgt cgcgcggcgc gatcgtgaag gtgggcgcgg cattcccgat
     4981 cctcagcagg caggtcgagg gtgtggcgtc cttttcggac gagatcgccg atgttcgccg
     5041 caagatcgag ccgtctggtg acatcgcgga ccacgccagc ccggcgctct cccagatacg
     5101 cgatcgcctt cgacggcaga agcagcgcct gcgctccacg ctcgattctc ttctacgcgg
     5161 ccgcgacacg tcgaaatacc ttcaggagca ggtcgtcacc gatcgcaacg ggcggtacgt
     5221 gttgacggtg cgcgccgagc atcgttcggc gatcccgggc atcgttcacg gcggatcgac
     5281 gagcggcgcg agcctcttcg tcgagccgct cgagacggtc gagatcaaca acgacatcgt
     5341 cgcgctcgaa gagcaggaag cggaggagat ccggcggatc ctgctggcgc tcaccgatgc
     5401 ctttcgcgcc cggccggccg aacttgcgcg cacgatcgag gtggcgaccg agctcgacgt
     5461 catccaggca cgcgcgcggc tgtccctgct caccagctcc gtagaaccca tcgtcgcggc
     5521 ggacggcacg ttcgagctgc gcggcgcgag acacccattg ctggtgttac ggggaaaatg
     5581 gggtcgggag tcatttttcg aggacgctgc gccaggcgac gccatgaaaa atgactcccg
     5641 accccatttc aattctgtcg tcccggtcga catcctcctg tcgccgccca cccggatcct
     5701 cgtgatcacg gggccgaaca cgggcggcaa gaccgtggcg ctcaagacgg cgggactcct
     5761 ggccgccatg gcgcagtcag gcctccacgt tcccgccgaa cccggatcgc ggatcccgat
     5821 cttcaagtcg ctcttcgccg acatcggcga cgaacagtcc atttcggcga gcctcagcac
     5881 gttctcggct cacgtgacga acctcgtctc catggaccgt gatctcgccc ttcccgccct
     5941 cgtgctgctc gacgaggtcg gggcgggcac cgatcccgtt gaagggggcg cgctcggcat
     6001 cgccgtcatc gatcatttca ggcggcgcgg cgcgcacctg gtggcgacca cgcattacga
     6061 ttcgctgaag tcgtacgcat cgaccacgga cggcgtggcg ggcgcggcgt tcggattcaa
     6121 ccccgagacg ttcgcaccga cctaccggct cctctacggg tcccccggac gcagcctggc
     6181 gatcgagatt gccgcgcggc tgggcatgcc tccgagcgtc atcgcggcgg cgcgcgaaaa
     6241 cctcagcgac cgcgagaagc agctggccga acacctggcc cgaatcgacg acgagatgcg
     6301 gcggctcgag gccgagcatc gagcggtggc cagggagcgc gcgacggtca gcgagaccga
     6361 acggcacctc agggcgcgcg aggagtcggt gcgcgagagg gagaacacct acaagcgccg
     6421 gctcgatgcc aagctcgatg agcagctccg cctgggccgg cgcgagatcg acacgatcat
     6481 cgaaggtctc aaggcaaaga cggcggagct gtcggagcag gcggagcggc gcatccggct
     6541 caacacgggc gacgcgggcg ccgtcaaggc cgacgcccgg gcggcgatcg agcgcgtggt
     6601 gtcgcggctc aagacgggac cggacctgcc ggagtcggcg tctcaccctt cagcgcgcga
     6661 ccatcgagcc aggatcgaac ccggaatgag agtcgtggtg ggggctctcg gcttcgaggg
     6721 cgtcgtcgtc gagattcacg gcaagcaggc ggaaatcgac gtgaagggca agcggctgcg
     6781 cgccgcggtt caggatcttc gcctggctgg cgcgggcggc ggcgctcccg cccggccgtc
     6841 ggtgcgcgtg aacgtggacc ttcagccccg ccagggatcg ctctcggaag tcaacgtgat
     6901 cggctgtacc gtggatgaag cgatcgcgcg cgtcgaaaaa ttcctggacg attcgacggt
     6961 gagcgatcag cagatcgtgc gtatcattca cggtcacggc acgggccagc tgcgccgcgg
     7021 cctcgcgggg ttcctgaaag aacatccgct cgtcgcaaaa ttccaggcgg ctccgacgga
     7081 ccagggtgga tcgggagtga cggtggtgga gttgaaggac tgacgagttt tgagtcttga
     7141 gttttgagtc tcgagttttg agttccatgg ccctgtaccc ccagtctttc atcgaggacc
     7201 tcaaacacca cgccgacatc gtcgtggtga tccaggacta cgtgtcgctc aagaaggcgg
     7261 gcacgagcta caagggcctg tgcccgtttc acggcgagaa gacgccgtcg ttccacgtca
     7321 atcgggacaa gggtttcttt cactgcttcg ggtgcggcgt cggcggcgac gtgttcaagt
     7381 ttctcgagct gcacgagaag gtcggatttc aggacgccgt gaagcagctc gcgcaacggt
     7441 tcggcctgac gcttcccgaa ctcgaacaga gcgacgagca gcgcgcgagc gccgccgagc
     7501 gcgagtcgct cctcaagatc cacgaggctg ccgccgcctg gtttcgcgag cagctgttgt
     7561 cgccggcagg cgtgcggatc cggcgtcaga tcgccggccg cggcgtcacc gatgccacct
     7621 ccgaagcgat gggactcggg tttgcgccgc cttcacgcga cgcgttgatg cagaggctga
     7681 cgacgcaggg gttcagcgac gggcttctga ttcgctcggg gctcgtgacc aggcgcgacg
     7741 acggcagcat cgtggatcgg ttcaggaacc gcctgatcat tccgatccgc cgcgacaccg
     7801 gatcggtcat cgccttcgga gggcgcgcgg tggacgccga ccagcagccg aagtacctga
     7861 actcgcccga gacgccgatc tacacgaagg gccggacgct gtacgggctg gatctcagca
     7921 aggcggcgat ccgcgaacgg gggtttgccg tgctcgtcga gggctacttc gatttcggcc
     7981 aggtgcatca ggcgggcttc ccggctgtgg cctcctgcgg gacggcgctg acgccgcagc
     8041 aggttcagca gctgcggcgg ttcacgtcga gggtcgtgct cagcttcgac cctgatgcgg
     8101 cgggccaggg cgccaccgaa aaatcgtgcg agctgttcgt cgcggagggc ttcgacgtca
     8161 acgtggcggt gctgccgtcg ggccaggatc cggacgcata catcaggtcg aacggaccgg
     8221 ccggttacgg tgaacgactg aagcagtcca agtcgtatct ggagtatctg ctggaccggg
     8281 cgagcgccgg tcacgacttg aactccgatg agggcagggt gagattcctg gcggccatgc
     8341 tcccgaccgc tgcgcggatt ccggacgccg cgatgcggga ccggtttgcg gaccgcctgg
     8401 cgttcaaggc caacgtgacc gacgaagtgg tccgggccaa gattcgggag gcggccgtcc
     8461 agcggcagac caccatttcg gccaatgccc tgccgagtgt ggggcgcgtc accaaggccg
     8521 aaaaagggct gatttggtgg ctcgttcacg accctggtcc cgccctgacg gcgctcgatg
     8581 agctcaatcc gtcggatttc gaggcgctgg cggccggatc ggtgctggat ctcgcgcgaa
     8641 aactgaacga aaatagaggt ttctctcctt ctgtgctgct cgagcgtcta aacatggtgg
     8701 aggcccaggt ggtcaccgca attgcgagtg agccggagcc gcccgccctg tcgctcagtt
     8761 tctgcgtacg cgagcttcgc cgcggtcgat acgagcgcga gcgggccgcc gtgcagcgcg
     8821 agattgccga gctccagacc aggggggcgg ccagcggagt ggaactggat gccttgctcg
     8881 tgcgcaaggg cgacctcggc cggctcatac aggcgctcgt gttatcggag gactgacctt
     8941 gtcgattgaa gagaaatacg acgaagtacg acagctgatc acagtcggca aggaaaaggg
     9001 ctacctgctc tacgacgagg tcaacgaact gctcccctcc gagatcacat cgtcggaaga
     9061 gctcgacgac ctcttcaacg ccttcggcag cgcgggcatc gaggtcgtcg actccgacaa
     9121 ggcgtaccgc ggcgacaagg atttcgatcg cgaaggatcg ggcgacgagc cggagctcga
     9181 cctgacgccg ggcgcgctcg acaagaccaa cgacccggtc cgcatgtacc tgcgggagat
     9241 gggcaccgtg ccgctgctga cgcgcgaggg cgaggttgcc atcgccaagc gcatcgagcg
     9301 cggcaagctc gccgtgatca agtcgatctc gcgcacgccg acgatcgcca agcgcatcct
     9361 catcatgggc gatcagctcc gcgcgggcga gcggacgatc cgcgagctgg tgatcttcag
     9421 tgacgaggag atcaccgacg aagtgatcca ggagcgggcg gtcgaagtcc tcggccagat
     9481 cgaggccgtg cgcaaggcgc ggggcaacta cctcaagctg ggggagaagc tcgacgaggt
     9541 cacgaagaag gacaagcgcc ggttccgccg ggcgcggctg aagacgatgc gggcgtgggt
     9601 cgaagtgtcg cgccgcatcc gcgacatcga gttcaccgaa tcgatcaagc ggcggctcgt
     9661 ggacgaggtg aaggacgcgg ttgacgccgt ccagaaggtc cagcgcgagg ccgagcacct
     9721 cgacaagcag atcagcccgc gggtgaagct gaagggaccc aggctcaggg aggaagagaa
     9781 gaaggcgctc cagaagcggt tgaaggacat ccgcgtccag gtcaagcaga tgatcgacga
     9841 cctggaggag acgcccgagc ggctgcggcg cacgatcgag gtgattcagc gaggcgaggc
     9901 gcaggccgag caggccaaga aggagctggt cgaagccaac ctccggctcg tagtctcgat
     9961 tgcgaagaag tatacgaacc gcggccttca gttcctggac ctgattcagg aaggcaacat
    10021 cggcctcatg aaggcggtgg acaagttcga gtaccggcgc gggtacaagt tctcgaccta
    10081 cgccacgtgg tggatccggc aggcgatcac gcgcgccatc gccgatcagg cgcggacgat
    10141 ccggatcccg gtccacatga tcgagacgat caacaagctg attcgtacct cgcgggcgct
    10201 cgtgcaggag ctcggcaggg agccgacctc cgaggagatc gccaagcgaa tggacatccc
    10261 ggtggccaag gtccggaagg tgctcaagat cgcgcaggag ccgatctcgc tggagacgcc
    10321 gatcggcgag gaggaagact cgcaccttgg cgacttcatc gaggaccgga acatcgtgtc
    10381 cccggccgaa gcggtcatca acctgaacct caaggagcag accgaggcgg tgctgaagac
    10441 gctcacgccc cgggaggaaa aggtcatcaa gatgcggttc ggcgtcggcg acggcagcga
    10501 gcacacactt gaagaggtcg gtcagaactt cgcggtgacc cgcgagcgca tacggcagat
    10561 cgaggccaaa gcgctccgga agctgcggca cccctcgcga tcgcgtaagc tgaaggcctt
    10621 tctcgaaggc agaacgtaac ccggaggcgg tggggcttgc cccgccgtag ccgcaaagcg
    10681 gcgaaggcgg gcccgtagct cagtggttag agcggccggc tcataatcgg ttggtcccag
    10741 gttcgaatcc tggcgggccc actcattgcg atgcgtgatg tcccctgatc tcgagcgtct
    10801 catcaggctg cagcacctcg aagccgccat cgcggaggcc agggcggcca tcgcgctgca
    10861 ccctcagcgc ctcgccgcgc tggatgcccg ccttgaggaa gcgaggcggg ccgtcgatga
    10921 cgtcaagcag cgcctcaaga ccaatcacga cgcccgcctc gagctggaca agggcatcgc
    10981 gctctaccag ggacggctga ccaagtacaa ggaccagctg tccgaggtca agaccaaccg
    11041 ggaataccag gcgatgcagc acgagatcgc cgcggcgcag aacgacctcg gggccgtcga
    11101 agagaaggtg ctcgtacgga tgatggaggg cgacgacctg acggccaccg ccaagcgggc
    11161 cgaggcggcg ctcgcggcgg caaccaaagt cgtcgaagcg gagaagaaga cgctcgccga
    11221 agagctcgcg tccaccgagt cgtcgctcgc caaggccacg gagggccgcg cggcgctcgc
    11281 cagggagatg gaaccgagac tcgtcgccct gttcgagcag gtggccagag tccggaaagg
    11341 cgtcgcgatc tgcgtcgcaa cgaaggacgg tctctgttcg ctgtgccacg tgcggctcag
    11401 gccgtcggtc ttccagcagg tccgccacaa cgacacgatc ctccagtgcg agagttgcca
    11461 acgggtgctg tactggatcc cgccgccctc gcccgtctcg ccccccgtcg tgcacgtgtg
    11521 accgctcgac tcaccaacgt caaagcgctc acggtcatcc cgagcgtagt cgagggatga
    11581 cccagccctc gttgttcgga ttagagaccg gggggtccgc cgtcgccaac atcgacggcg
    11641 gctcccgcgg caaccccggg ccagcgggtt acggagtccg catcgagcgt gaagacggca
    11701 cgctcgtcga cttgaagcag tcgcttagcg cgtgcacgaa caacgtcgct gagtacagcg
    11761 ggcttctcgc tgcactgcgc tgggcggccg atcacggcgt caccacgctc cgcgtgcgat
    11821 ccgattcgga gctgctcgtc aagcagatga aaggcgagta ccgggtgaag aaccccgggc
    11881 tgcagccgct ctacgaggag gcgagagcgc ttgctcgccg catcggacgc gtgacgttcg
    11941 agcacgtccg ccgcgagttc aacaaggacg ccgaccggct cgcgaacgag gcgatggacg
    12001 aggcggagac ttcgccgtaa ttcagaaagt acagtcactt cgtgtgcttg gcgcgagtgg
    12061 tcacatagag atcgaaaccg ccaaccccgc cggtgcgatt cgacacgaag tacagcgttt
    12121 ctctgtctga ggtgatttcc ggatctccgt ctatagcaag gctgtttacc agcggcccca
    12181 ggttggtggg ctccgaccac gcatcggaca ccgtatcgcg agtggctgtc cacaagttga
    12241 ggagaccaaa gctgccgggt ctgttcgact ggaagaacac ttcgagcccg tcgaatctca
    12301 cggatgggct tcggtcaagt gcggtgctgt cgagctccac aactggtctc gccggcccga
    12361 acgtcccatc cggcagcaga tcgctgacat agattccgtc cgttgcgttc gagaagaaaa
    12421 gctgcggtac accgacatca tcgttttcaa agaaattcgg gttctgctcg ttgccgggag
    12481 aattgatgcc ggaacccgcg ttcactggag gctgccagcc gaagtcgtcg cggacaatcg
    12541 cgcggtagga cacccagatg tccccggatc cgaaaccggg gcgcccactg gtgaagaaga
    12601 gccagtgccc gtcacgcgac aagctaggct gaaagtcgcc aaagatcgtg ttgacgaccg
    12661 ggccgaggtt ggtcggaggg ccccacgact ctcccacgct gttgcgttgg gacacccaga
    12721 tgtcgttccc gccaagtcca tcgggcctgg tggagctgaa gtacaaactc agttggtcct
    12781 tggacacggc gggagctgtg tcaccgaacg cggaattgac gttgggccca aggttcaccg
    12841 gtgcggacca gtctgagaag tcgggggccg ccgtcggaga cgacaccgcg agcagcacga
    12901 aacccatcgc cgtcagggcg aatcgtcggg aaaccatggg aactaccctc cttgacgagc
    12961 ttccggccga ggtgccagag ttcgcgatct ggcgtcacta gtggagcgct tcagcgaaac
    13021 gtaaagcggg ccgcccgtcg gttggcccgc gaaatcggcg tcgcagggct gtccgactcg
    13081 ctgcgggttc agttgatcca gtccactagt agacgcggga gatctggttt gggtgtgtca
    13141 cgcgcgggac aaggggagcg cacacgtttg cggacggcgt cgaccggaca ggtgaccggc
    13201 ctgctgcggg cgtgggccgc cggacacccg gaggcgcagg accaactgct gccgctcgtc
    13261 taccaggagc ttcgccggcg cgccgccggc catcttcgcc gcgagcgcaa ggaccacaca
    13321 ctccagccca cggcgctcgt ccacgaagcc tacctgcgcc tgctcggcca gcgcgcggac
    13381 tggcagagcc ggacgcagtt tttcgcgctc gcgtcgcgga tgatgcgccg gatcctcgtg
    13441 gaccacgccc gggcccgcg
//
LOCUS       DBNGSH010000885         2404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1978275, whole genome shotgun sequence.
ACCESSION   DBNGSH010000885 DBNGSH010000000
VERSION     DBNGSH010000885.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1978275"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="AC16CY_20390"
     CDS             <1..336
                     /locus_tag="AC16CY_20390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HIR3356222.1"
                     /translation="DAARSARAGALRNRGVSVLPVAGESMTAALSMLSGLDVQSVVLE
                     GGAQVQRSAWDEGVVDYVQLYLAPVWLGEEGVPLLEGCTFSPMALLERRVEQLGPDVL
                     IEGYVHRPH"
     gene            317..925
                     /locus_tag="AC16CY_20395"
     CDS             317..925
                     /locus_tag="AC16CY_20395"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR3356223.1"
                     /translation="MFTGLIEAVGEVSEITPTSSGYRLRIHTPERLGLEPGDSVAVNG
                     VCLTATQCDEQGTDADVGPETARVTTLGRLQRGQKVNIERSVRADGRFDGHFVQGHVD
                     GTGVVREVRDDGDSHWISIGIPPELEPFLILRGSVAVDGVSLTVARLSEACFDVMIIP
                     FTWANTSLSSLGAGDLVNLECDMVGKYVARSMALAARSGATT"
     gene            922..2175
                     /locus_tag="AC16CY_20400"
     CDS             922..2175
                     /locus_tag="AC16CY_20400"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR3356224.1"
                     /translation="MTTTPSALRIAKGARRRGPFATIEQAIEAIRAGQLVVVVDDEGR
                     ENEGDLTMAAEKVTPEAINFMARYGRGLICLAMTPDRLDQLQVPLEVSDNSSRRDTAM
                     CVSIDARDGTTTGISAADRAETIRVAIAPKTRPRDLQRPGHVFPLRARKGGVLVRAGH
                     TEAAVDLAGIAGLTPAGVICEIMNEDGTMARVPELARFARKHGLLMMTIADLIQYRMR
                     TERLVKRAASADLPTLHGAFRIYAYESLLDSMTHVALVRGDIGDGKDVLVRVHSKCLT
                     GDVFHSARCDCGPQLDTAMQRVAKEGRGVLLYLNQEGRGIGLVNKIRAYELQDQGLDT
                     VEANERLGFKADQRDYGIGAQILSDLGVRTMRLLTNNPRKFVGLQGYGLSVSEAVPLE
                     IPASDHTRRYLKTKKDKLGHKLSTV"
     gene            2221..>2404
                     /locus_tag="AC16CY_20405"
     CDS             2221..>2404
                     /locus_tag="AC16CY_20405"
                     /inference="COORDINATES: protein motif:HMM:NF016430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HIR3356225.1"
                     /translation="MHERAERLAGPLVTANREIEHDFEARLGDSATLAFRVAYSVLRH
                     REDAEDVTQEAFAKAYR"
BASE COUNT          415 a          753 c          865 g          371 t
ORIGIN      
        1 gacgccgcgc gatccgcgcg cgccggggcg ctcaggaaca ggggtgtcag cgtgctcccg
       61 gttgcagggg aaagcatgac cgcggcgctc tcgatgctgt cgggcctcga cgtgcagagc
      121 gtcgtgctgg aaggcggagc gcaggtgcag cgatcggcct gggatgaagg cgtcgtggat
      181 tacgtccagt tgtatctcgc gccggtctgg ctcggtgaag aaggcgtccc gctgctcgag
      241 ggctgcacgt tctcgccgat ggcgctcctc gagcggagag tcgagcagct tggaccggac
      301 gtgctgatcg aaggttatgt tcaccggcct cattgaagcc gtcggcgagg tttcagagat
      361 cacgcccacg tcgtccgggt accggctgcg aatccacacg ccagagcggt tggggctgga
      421 gccgggggac agcgtggccg tcaacggcgt gtgcctgacg gcgacccagt gtgacgaaca
      481 gggcacggac gcggacgtcg gacccgagac cgcgcgggtg acaacgctcg gccgcctcca
      541 gcgcgggcag aaggtgaaca tcgagcgatc ggttcgcgcc gacggccgct tcgacggcca
      601 cttcgttcag ggacacgtcg atggcacagg cgtcgtgaga gaggttcgag acgacggcga
      661 ctcgcactgg atctcgatcg ggatcccgcc ggagctcgag cccttcctga ttctgagagg
      721 gtccgtcgcc gttgacggag tgagcctcac cgtcgcgcgc ttgagcgagg cgtgtttcga
      781 cgtcatgatc attccgttca cctgggcgaa caccagcctg tcgtcgctgg gcgcgggcga
      841 cctggtgaat ctcgagtgcg acatggtcgg caagtacgtg gcgcggtcga tggcgctggc
      901 cgcccgcagc ggggcgacga catgacaacg acaccatcgg ctctccggat cgcgaagggt
      961 gcgcgccggc gcggaccctt cgcgaccatt gaacaggcga tcgaggccat tcgggccggg
     1021 cagcttgtcg tcgtcgtgga cgacgaaggg cgcgagaacg agggcgacct gacgatggct
     1081 gccgagaagg tgacgccgga ggcgatcaac ttcatggcga ggtacggccg cggcctgatc
     1141 tgtctggcga tgacgccgga tcgcctcgac cagctccagg ttccgctcga agtgagcgac
     1201 aactcgtcgc gccgggacac ggcgatgtgt gtgtcgatcg acgcgcgcga cgggacgacg
     1261 accgggatct cggctgctga ccgcgccgag acgattcgtg tcgcgattgc ccccaagacg
     1321 cggccgcgcg atctgcagcg gcccggccac gtgtttccgc tgcgcgcccg gaagggcggt
     1381 gtgctggtgc gggccgggca caccgaggcc gcagtggacc ttgcggggat cgccggcctc
     1441 acgccagccg gcgtcatctg cgaaatcatg aacgaggacg gcacgatggc gcgggtgccc
     1501 gagctggcga ggtttgcccg gaagcacggc ttgctgatga tgacgatcgc ggacctgatt
     1561 caatatcgca tgcgtaccga acggctcgtg aagcgggcgg catcggccga tctgccgacg
     1621 ctgcacggag catttcgcat ctacgcgtac gagagcctgc tcgacagcat gacccacgtg
     1681 gcactggtgc gcggcgacat cggcgacggt aaggacgtgc tggtccgcgt gcactcgaag
     1741 tgcctgacgg gcgacgtctt tcattcggcc cggtgcgact gcggccccca gctcgacacc
     1801 gccatgcagc gcgtggccaa ggaagggcgg ggcgtgctgc tgtacctgaa ccaggaaggg
     1861 cgcgggattg ggctggtgaa caagatccgc gcctacgagc tgcaggacca ggggctcgac
     1921 accgtcgagg ccaacgagcg cctcgggttc aaggcggacc agcgggacta cgggatcggc
     1981 gcgcagatcc tgagcgacct gggcgtgaga acgatgcggt tgctcacgaa caacccccgc
     2041 aagttcgtcg gcctgcaggg ctacggcctc tccgtgtccg aagccgtccc gctggagatt
     2101 cccgccagcg accacacgcg gcggtacctc aagaccaaga aggacaagct cggtcacaag
     2161 ctgtctacgg tgtaagtgaa ccgcccgcgc ccccgcggcg tcttgcctgg gtgaagcctg
     2221 atgcatgagc gcgccgaacg ccttgcgggc cccctcgtga cggccaaccg tgagatcgag
     2281 cacgacttcg aggcccgtct cggtgattcc gcaacgctgg cattccgggt ggcgtacagc
     2341 gtgctccggc acagggaaga cgccgaggac gtcacgcagg aagcgttcgc caaggcgtac
     2401 cgca
//
LOCUS       DBNGSH010000886         3782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1129300, whole genome shotgun sequence.
ACCESSION   DBNGSH010000886 DBNGSH010000000
VERSION     DBNGSH010000886.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1129300"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2264
                     /gene="nifJ"
                     /locus_tag="AC16CY_20410"
     CDS             <1..2264
                     /gene="nifJ"
                     /locus_tag="AC16CY_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR3356226.1"
                     /translation="GEETDFHPQGYFVYDSKKSGAITVSHLRTSTRPIRSAYLVDRAR
                     FVACHQFEFVDKIDVLEHAAPGATFLLNAPFPADEVWNHLPREAQEQIIDKKLRFFAI
                     DAYDLAKRAGMGTRINTIMQTCFFAIAGLLPRDEAIGHIKKAIEKTYGKRGPEVVRRN
                     WEVVDQSLAHLREIPVPDAVNASHGRPPLVSEQAPDFVQKVTAVMLAGKGDQLPVSAF
                     PVDGTWPVATAKWEKRNIAAEIPVWDTRICIQCNQCALVCPHAAIRAKVYDESALGGA
                     PPTFKSTPYRGNEYKGKHFTIQVAPEDCTGCNLCVNVCPAKDRTNPRHKAINMQPQAP
                     LRSSERVNYDFFLNLPEPDRAAIARIDHKQSQFMEPLFEYSGACAGCGETPYLKLLTQ
                     LFGDRLLIANATGCSSIYGGNLPTTPYTTNRDGRGPAWSNSLFEDNAEFGFGFRLALD
                     AHVATSRRLLQEIAPQVGDGLATAILDADQASEAGIAEQRRRVEALRQRLAGIATAEA
                     RRLETLVDYLVKKSVWLVGGDGWAYDIGYGGLDHVLANRRDVNILVMDTEVYSNTGGQ
                     QSKATPLGAAAKFAAAGKPIGKKDLGLLANMYGHVYVAKVAFGAKMAQTAQAFLEAES
                     YAGPSLIIAYSHCIAHGYDMANGAAQQKLAVDTGVWPLYRFDPRRLVKGEPPLKLDYG
                     PPKGRVVDYLRNESRFRTVERADPALYKSFVAESQAAAQRRYAVYQQLAGITVPVIQP
                     AADEPAQPESKGE"
     gene            2264..3241
                     /locus_tag="AC16CY_20415"
     CDS             2264..3241
                     /locus_tag="AC16CY_20415"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR3356227.1"
                     /translation="MDLTTTYLGMRLPHPLIVGAGPLGDDLETVRALEDAGAALLVLR
                     SLYEEEITGEQMDAFLSSESHSDSFAEAGTYAPDPILALGPDEYLEHLRLIKSAVRIP
                     VMASLNGVTPGGWISYAKQLEEAGADGLELHIYHAASDMTISAADVERQAVEIVREVK
                     RSLRIPVAVKLAPLLTAFAHYARQLDGAGADGLVLFTRFHRVDIDVDELEVIRSLPLS
                     DSSELPMRLRGAAALSGRVKASLALTGGIHTGLDVIKATMAGAHVTQMVSALLRHGPA
                     HLATVRRDVESWMEEHEWSSLGEMRGNMSLERIPDPAAYERANFRMALR"
     gene            complement(3277..>3782)
                     /locus_tag="AC16CY_20420"
     CDS             complement(3277..>3782)
                     /locus_tag="AC16CY_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3356228.1"
                     /translation="SNDPIPPYTWTGGPPPEEKNFVPDLHFVEISQDRRVYIGERGQN
                     RIQVFTTDGRFLQEFSVAPNTPARGEGCGGLNNTKMPPCGTTYKLAISRDPAQKYLYV
                     ADGTNNRVWILDRASGKTLGSFGGNGRYAGQLHWINAIATDSTGNIYTGEVEQAKRIQ
                     KFEPVRK"
BASE COUNT          650 a         1286 c         1234 g          612 t
ORIGIN      
        1 tcggcgagga gaccgacttc catccccagg gctacttcgt ctacgactcc aagaagtcgg
       61 gcgccatcac cgtctcccac ctgcggacga gcacgcggcc aatccggtct gcgtacctcg
      121 tggatcgcgc gcgcttcgtg gcctgccatc agttcgaatt cgtggacaag atcgacgtgc
      181 tcgagcacgc ggcgcccggc gccacgttcc tgctgaacgc tccgttcccc gccgacgagg
      241 tctggaacca cctgcccagg gaggcgcagg aacagatcat cgacaaaaag cttcgcttct
      301 tcgccattga cgcgtacgac ctcgcgaagc gtgccggcat gggcacgcgc atcaacacga
      361 tcatgcagac gtgcttcttc gcgatcgcgg gcctcctccc ccgcgacgag gcaatcggcc
      421 acatcaagaa ggcgatcgag aagacctacg gcaagcgcgg accggaggtc gtgcgccgga
      481 actgggaggt ggtcgatcag tcgctcgcgc acttgcgcga gattcctgtg ccggacgcgg
      541 tcaacgccag ccacggtcgg ccgccgctcg tatcagagca ggcccccgat ttcgttcaga
      601 aggtcaccgc ggtcatgctg gccggcaagg gcgatcagct tcccgtgagc gcgtttccgg
      661 tggacgggac gtggccggtg gcgaccgcca aatgggagaa gcggaatatc gcggccgaga
      721 taccggtgtg ggacaccagg atctgcatcc agtgcaacca gtgcgcgctc gtctgcccgc
      781 acgccgcgat tcgggcgaag gtgtacgacg agagcgcgct cggcggcgcg ccgccgacgt
      841 tcaagtccac gccgtacagg ggcaacgagt acaagggcaa gcacttcacc atccaggtcg
      901 cacccgagga ctgcaccggc tgcaatctgt gcgtcaacgt ctgtcccgcc aaggatcgca
      961 ccaaccccag gcacaaggcg atcaacatgc agccgcaggc gccgctgcgg agcagcgagc
     1021 gcgtcaacta cgacttcttc ctcaacctgc ccgaaccgga tcgcgccgcc atcgctcgca
     1081 tcgatcacaa gcagtcgcag ttcatggaac cgttgttcga gtactcgggc gcgtgcgccg
     1141 gctgcggcga aacgccgtac ctgaagctgc tcacgcagct cttcggcgat cgcctcctca
     1201 tcgcgaacgc gaccggctgc tcgtcgattt acggcggcaa cctgccgacg acgccgtaca
     1261 cgaccaaccg cgacgggcgc ggtccggcat ggtcgaactc gctcttcgag gacaacgcgg
     1321 agttcgggtt cgggttcagg ctggcgctcg atgcccacgt cgccacgtcg cgccgcctgc
     1381 ttcaggagat cgcgccgcag gttggcgacg gcctggcaac ggcgatcctg gatgcggatc
     1441 aggcgagcga ggccggtatc gccgaacagc gccggcgcgt cgaggcgctc cggcagcgcc
     1501 tcgccgggat cgcgaccgcc gaggcgcgca ggctcgaaac gctcgtggac taccttgtca
     1561 agaaaagcgt gtggctcgtc ggcggagacg gctgggcgta cgacatcggc tacggcggcc
     1621 tcgatcacgt gctggcgaac cggcgggacg tcaacatcct ggtcatggac accgaggtct
     1681 actcgaatac cggcgggcag cagtcgaaag cgacgccgct cggcgcggcg gccaagttcg
     1741 ccgccgcggg aaagccgatt ggaaaaaagg acctgggcct gctggccaac atgtacggcc
     1801 acgtgtacgt ggccaaggtc gccttcggcg ccaagatggc ccagacggcg caggcgtttc
     1861 tcgaagccga gtcctatgct ggtccgtcgt tgatcatcgc ctacagccac tgcatcgccc
     1921 acgggtacga catggccaac ggcgcggcgc agcagaagct cgccgtggac accggcgtct
     1981 ggccgctcta ccggttcgat ccccgccggc tcgtgaaggg cgagcctccg ctgaagctcg
     2041 actacggccc acccaagggg agggtcgtcg actatctgcg gaacgagtcg cgcttccgga
     2101 ccgtggagcg cgccgatcct gcgctgtaca agtccttcgt cgccgagtcc caggccgcgg
     2161 cccagcggcg atacgccgtg tatcagcagc tggccggcat cacggtgccg gtgattcagc
     2221 cggcggccga cgagccggcg cagcctgaat cgaaggggga gtgatggatc tcaccacgac
     2281 gtacctcgga atgcgcctgc cccatccgct gatcgtcggc gccggtccgc tcggcgacga
     2341 cctggagacg gtgagggccc tcgaagatgc cggcgccgct ctgctcgtgc tccgttcgct
     2401 gtacgaggag gaaatcacgg gcgagcagat ggacgcgttc ctcagctccg aaagtcacag
     2461 cgattcgttt gcggaagcgg gaacctacgc gcccgatccg atcctggcgc tcgggcccga
     2521 cgagtacctg gagcatctgc ggctgatcaa gtccgccgtg cgaatcccgg tgatggcgtc
     2581 cctcaacggc gtcacaccgg gcggctggat ttcctacgcg aagcagctcg aagaggctgg
     2641 cgcggacggc ctcgagctcc acatctacca cgcggcgagc gacatgacga tcagcgccgc
     2701 cgacgtcgag cgtcaggccg tcgagatcgt gcgcgaggtg aagcgcagcc tccggatccc
     2761 ggtcgctgtc aagctggcgc cgctcctcac agccttcgcc cattacgcca gacagctcga
     2821 tggggccggc gccgatggcc tcgtgctctt cacgcgcttc caccgggtcg acatcgacgt
     2881 ggacgagctc gaggtgatcc gttcactgcc cttgtcggac tcgagcgagc tcccgatgcg
     2941 tcttcgcggc gccgctgcct tgtccggacg cgtcaaggca tccctcgcgc tcaccggcgg
     3001 catccatacg ggtctcgacg tcatcaaggc cacgatggcc ggcgcgcacg tcacgcagat
     3061 ggtgtcggcc ctgcttcgtc atggccccgc tcatctcgcc acggtccgca gggacgtcga
     3121 gtcgtggatg gaggagcacg agtggagctc gctcggcgag atgcgcggca acatgagtct
     3181 cgagcggatc cccgatcccg cggcgtacga gcgcgcaaac ttccgtatgg cactgaggtg
     3241 acatgagggc gctcgcgcca gggggcacgg gcgccgtcac ttccgcaccg gctcgaactt
     3301 ctggatccgc ttcgcctgct ccacctcgcc ggtgtagatg ttgcccgtcg agtccgtggc
     3361 gatcgcgttg atccagtgga gctgcccggc ataccggccg ttgccgccga acgatcccag
     3421 cgtcttcccg ctcgcgcgat cgaggatcca cacgcggttg ttcgtcccgt ccgcgacgta
     3481 caggtacttc tgcgcgggat cgcgcgagat cgccagcttg taggtcgtgc cgcagggagg
     3541 catcttcgtg ttgttcaacc cgccgcaccc ttctccacgc gccggcgtgt tgggcgcgac
     3601 ggagaactcc tgcaggaacc tgccgtccgt cgtgaacacc tggatccggt tctgcccgcg
     3661 ctcgccgatg tagacgcgcc gatcctgcga gatctccacg aagtgaagat cgggcacgaa
     3721 gttcttctcc tcgggaggcg ggccgccggt ccacgtgtat ggcggaatcg ggtcgttcga
     3781 ga
//
LOCUS       DBNGSH010000887         2612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1468595, whole genome shotgun sequence.
ACCESSION   DBNGSH010000887 DBNGSH010000000
VERSION     DBNGSH010000887.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1468595"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="AC16CY_20425"
     CDS             complement(<1..832)
                     /locus_tag="AC16CY_20425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3356229.1"
                     /translation="MSGWAVRIGVMAIVSTTAAVAQQPVFRADAEIVSLAATVTDGRG
                     EFLSSLGREDFEIYEDGEKQVIQHFARGDDLEEGPELHIGLMFDTSGSMTDDIRLSRT
                     AAIRFLNVMKHAKDITYVDFDTEVRVTRFTQQDFPRLVERIRSREPEGFTAMYDALGV
                     YLDGASRLPGRKVLVVFTDGGDTRSAIRFGDVITLIRASDVTVYSIGFLQSQPNSART
                     EQRLRLTQIASESGGSAFFPSSVKQVDEAYDTIVAQVQAQYTLGYVSTNTAADGRWRK
                     V"
     gene            969..2396
                     /locus_tag="AC16CY_20430"
     CDS             969..2396
                     /locus_tag="AC16CY_20430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR3356230.1"
                     /translation="MRCLAACQTELAIRMLDEILLPNFEVEFLVESKPLARRLHDSGL
                     NITCGDLKRTDTYLKADLSPGTCVVVEDNGRKSLRKVLQAIMDAGGTLVYALGVGTSH
                     TREREEELKGQFPELNYLPLSELFGGQLLTEFGRSLTRLKVQQYQRFFSDADRVLIIL
                     HNDPDPDALASGLALRNVLHRNRQTAVIAAVEGVTRPENQRMMNLLDIHVEIITPDQV
                     GEFDRVAMVDVQPHYFGGLIDRVDLVIDHHPEQPGYTSVYKDIRVDYGSTSTILSEHL
                     RAIDANISERVATAMLYAIKSDTLFFNRQANRVDIDAFSYLYPLADATLIRKMEGAEI
                     TMERLEYVLRAKQNGRMTEQVFCAFLGTTPREDFIPYVADFYLQLENVQWTIVAGIVN
                     GSIVMSVRNVGYSRNAGEFVRKYFQEIGNAGGHRAMAKAVVPLRAFQEKFGILQADEY
                     TERILALALEFLHEHQPADKKLVKA"
BASE COUNT          463 a          883 c          800 g          466 t
ORIGIN      
        1 cgaccttgcg ccagcgtccg tccgccgccg tgttggtaga gacgtacccc agtgtgtact
       61 gcgcttgcac ctgcgcgacg atggtgtcgt aggcttcgtc cacctgcttg accgaggagg
      121 gaaagaacgc gctgcctccg ctctcggacg cgatctgggt caagcgcagg cgctgctctg
      181 tacgggcgct gttcggctga ctctgcagga atccgatcga gtacaccgtt acgtccgacg
      241 cgcgaatcag cgtgatcacg tccccaaagc ggatcgcgct gcgcgtgtcg ccgccatcgg
      301 tgaagaccac gagcaccttc cggcccggga gccgggacgc gccgtccagg tacacgccca
      361 gcgcgtcgta catcgccgtg aagccttccg gctcgcggga gcggatgcgt tcgaccagcc
      421 ggggaaaatc ttgctgtgtg aaccgggtca cgcggacctc ggtgtcgaag tccacgtacg
      481 tgatgtcctt ggcatgcttc atcacgttca ggaaccggat ggctgccgtt cgcgacagcc
      541 tgatgtcgtc ggtcatgctg ccgctcgtgt cgaacatcag cccgatgtgc agctcgggac
      601 cttcttccag atcgtcgcca cgcgcgaaat gctggatgac ctgcttctca ccgtcttcgt
      661 agatctcgaa atcctcgcgg cccagacttg acaggaactc cccgcgcccg tcggtgacgg
      721 tggcggcaag gctcacgatc tcggcgtcgg cccggaagac gggctgctgg gcgacggcgg
      781 ccgtcgtgga cacaatggcc atcaccccaa tccggacggc ccatccgctc atggcgacat
      841 gatagctggt tggcgagcgc tcgccgtagg agcacgccgg gcggctgccg gcaggctgcc
      901 ggcggctggg cgtataatgg tggtttgtcg cctatgtcta gggtgctggc gcaggttacc
      961 ggcgcggcat gaggtgcctc gccgcctgcc aaacggagtt ggccatcagg atgctcgacg
     1021 agatcctgct ccccaacttc gaggtcgagt tcctggtcga gagcaagccg ctggcgcggc
     1081 gcctgcacga tagcggtctc aacatcacgt gcggggacct gaagcgaacc gacacctacc
     1141 tcaaagccga tctctcgccg ggcacgtgcg tcgtcgtcga ggacaacggg cggaagagcc
     1201 tccgcaaggt cctgcaggcc atcatggacg ccggagggac gctcgtctac gcgctggggg
     1261 tggggacgtc gcacacgcgg gagcgggagg aagagctcaa agggcagttt cccgagctca
     1321 actaccttcc cctctccgag ctctttggag gtcagcttct caccgagttc ggccggtccc
     1381 tcacacgcct caaggttcag cagtatcagc gcttcttcag cgacgccgat cgcgtcctga
     1441 tcattctgca caacgatccc gatcccgacg ccctggccag cgggctggcc ctgcgcaacg
     1501 tcctgcaccg caaccggcag accgccgtca tcgccgcggt cgaaggagtg actcgtcccg
     1561 agaaccagcg gatgatgaac ctgctcgaca ttcacgtcga gatcatcacc cccgaccagg
     1621 tcggcgagtt cgaccgcgtc gccatggttg acgtgcagcc tcactacttc ggcggcctca
     1681 tcgaccgcgt cgatctcgtc atcgatcacc atcccgagca gccgggctac acgtcggtct
     1741 acaaggacat tcgcgtggac tacggctcga cctcgaccat cctgagcgag cacctccgcg
     1801 ccatcgacgc caacatctcg gagcgcgtgg ccacggccat gctctacgcc atcaagtccg
     1861 acacgctgtt cttcaaccgt caggccaatc gcgtggacat cgacgcgttc tcgtatctct
     1921 acccgctggc cgacgcgacg ctgatccgca agatggaagg cgccgaaatc acgatggagc
     1981 gcctcgagta cgtgctcagg gcgaagcaga acggccgcat gacggaacag gtcttctgcg
     2041 cgttcctcgg cacgacgccg cgcgaggact tcatccccta cgtcgccgac ttctacctgc
     2101 agctcgagaa cgtccagtgg accatcgtcg ccggcatcgt caacgggtcc atcgtgatgt
     2161 cggtgcgcaa cgtcgggtac tcgcgcaacg caggtgagtt cgtccgcaag tacttccagg
     2221 agatcggaaa cgccggcggc cacagagcga tggccaaggc cgtcgtgccg ctgcgggcgt
     2281 tccaggaaaa gttcgggatc cttcaggccg acgagtacac cgaacgcatc ctcgcgctgg
     2341 cgctcgagtt cctgcacgag caccagcccg cggacaagaa gctcgtcaaa gcctgaacac
     2401 tcccccggtt cacacttctc gcttctcact tctcggttcg cgtttccgcc tccgccaagg
     2461 ctacggcggg acctcgccga agcgcttcgc gcgaaggcgg gcactttcag gttcgtgttc
     2521 tatcatggag accgcatggc cacgatcctg cagcgccttc ccgtcggtga acgggtaggc
     2581 atcgcattct ccggcggcct cgacacgagc gc
//
LOCUS       DBNGSH010000888         3873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4015466, whole genome shotgun sequence.
ACCESSION   DBNGSH010000888 DBNGSH010000000
VERSION     DBNGSH010000888.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4015466"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC16CY_20435"
     CDS             complement(<1..202)
                     /locus_tag="AC16CY_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3356231.1"
                     /translation="MPRVVRVTLAVAAFLVLLPALASAQEGQIAGTVRDASGGVIPGV
                     LVEVTSPALIEKVRSTTSDASGQ"
     gene            441..2153
                     /locus_tag="AC16CY_20440"
     CDS             441..2153
                     /locus_tag="AC16CY_20440"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3356232.1"
                     /translation="MRHSTSIAGLALAAALTASGEAGQRGTVRNSPTQAPAYLDVSRD
                     AGLAFTNVNGASAQKRLVETMGSGGLFFDFDNDGWIDIFLVDGGSEADPAVARTARHR
                     LYRNRRNGTFEDVTSSSGIRHREYGMGACAGDYDNDGLVDLYVTNVGPNVLYRNSGGG
                     AFTDVTQKAGVASALWGTSCAFADFDRDGRLDLFVTNYVEMGKDRDRFCGSTRPMVRQ
                     YCHPLVFQSAPNVVYRNAGNGTFTDVTARAAVAAQRGNGLGVAVADYDDDLWPDVFVA
                     NDGVPNFLFRNDGRGGFTDVALVAGVSSASDGRSRAGMGTAFGDVDGDGRLDLVVTNH
                     ETEMHSLFKNLGNGSFADATVESGVGPATLPYVGFGVVLADYDNDGDLDLAIVNGHVI
                     DNVALVRSGATHAQARLLLQNGGTGRFTDVSAQSGPAFGVSAVGRGLAAGDIDNDGDL
                     DLLATSNGGPAALLRNDGGNRSNALLVRLTGTMSNRDGIGARLRATTGARTQMRELTS
                     GSSYLSQNDARVHLGLGSAAMVDRLEIRWPSGRTETLEKVSANQIITVREREGIVGTA
                     PLAR"
     gene            2270..>3873
                     /locus_tag="AC16CY_20445"
     CDS             2270..>3873
                     /locus_tag="AC16CY_20445"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356233.1"
                     /translation="MTVMSTVRRGPKTLTTAAFLVAALTVLPAGAAAQRDPFIDAFVS
                     FHSALSGTYGDEGPKVAASLDQMAASLAAWDRTAAGAVAKLTSQRTNTAGDLALAYLD
                     WGRLDEALAALDSAIAIEPTRAAFHQLRGLILDASGRRDDAIAAFASAWALDPNDPIK
                     AYLLADRRGTDAGAGDLQPQLAALLASYGRGTPAGRAPFIPLTLIEDRAATTPIFSPA
                     PYAGGFALVAQGRYQDAIARFREAMAGDGLVADTPARSEPMSKGIAALREGRVGAAIE
                     LLEAAVAAAPRSSEAHRILGAAYGANRNHDKSVAHLNTAIGLAPRDERARVTLGRELA
                     DAGTFEEAERALRDTIAMLPASGEARWALANLYDKNGRGPDAVRELEAAASLTVMAGK
                     GAIYWRIAEISHVHQDYERVAGALSRRARLMPNEPIAHKDLGLAYNRMGRQDQALIEL
                     VMASLLGLEDAESLGVMGQLHLNAGRYATAEAVLRRAVALQPDAAQPRYALGSTLLRL
                     GKAEEAKSQLAEFQRLRAAALDEQRRT"
BASE COUNT          659 a         1333 c         1286 g          595 t
ORIGIN      
        1 actgcccgct tgcgtcgctc gtggtcgagc gaactttttc aataagtgcc gggctcgtga
       61 cttcgacgag cacgccaggg atgacgccgc cggacgcgtc gcgcaccgtt ccggcgatct
      121 ggccttcctg cgcggaggcc aacgccggca gcagcaccag aaatgccgcc actgccaggg
      181 tcactctcac aactcgtggc attacgacct cctcgagttt cccaaaccgc tttcagttgg
      241 acgaagccga gaaacgaacc agggttccca gggcgagacg cgttcctaca gggttcctac
      301 gggtttcctc cggagttcct acgcgggcgt gggcactatt tacgcctcat ggccgagtct
      361 gtcaagacga atgttaaaac catgtaaacc cggtgccaaa accgatctct tcgagcgtcg
      421 tatagtgact cctggggcgc ttgagacatt ccacctcgat agcgggtctg gcgctggcgg
      481 ctgccttgac ggcatcgggc gaggccggcc agcgcggcac cgtccggaac tccccgacac
      541 aggcgccggc ctacctcgac gtcagccgtg acgccggcct ggccttcacg aacgtcaacg
      601 gcgccagcgc gcagaagcgg ctggtcgaaa cgatgggctc cggcgggctc ttcttcgatt
      661 tcgacaacga cggctggatc gacatcttcc tcgtggacgg cggatcagag gcggaccccg
      721 cagtcgcgcg aaccgcgcgc caccggctgt atcggaatcg ccgcaacggc acattcgagg
      781 acgtcacgtc ctcatccggc atccgtcatc gtgagtacgg catgggcgcc tgcgccggcg
      841 actacgacaa cgacggcctc gtggatctct acgtcaccaa cgtcggtccc aacgtgctgt
      901 accgcaactc gggcggcggc gcattcaccg acgtcacaca gaaggccggc gtcgcgtctg
      961 cgctgtgggg aacgagctgc gcgtttgccg acttcgacag agatggacgc ctcgatctgt
     1021 tcgtcaccaa ctacgtcgag atgggcaagg atcgcgaccg cttctgcggc agcacgcggc
     1081 cgatggtgcg gcagtactgt catccgctcg tcttccaaag cgcgccgaac gtcgtgtacc
     1141 gcaacgcggg gaacgggacc ttcaccgacg tcaccgcgcg tgcggcggtc gcggcgcagc
     1201 gcggcaacgg tctgggcgtc gcggttgccg attacgacga cgacctgtgg cccgatgtgt
     1261 tcgtggccaa cgacggcgtg ccgaatttcc tgttccgcaa cgacgggcgc ggcggcttca
     1321 cggatgtggc gcttgtcgct ggagtgtcgt ccgcgtccga cggcaggtcg cgcgcgggga
     1381 tgggcaccgc attcggcgac gtggacggcg acggccgact ggatctcgtc gtgacgaacc
     1441 acgagaccga gatgcacagc ctcttcaaga atctcggcaa cggatccttc gcggatgcca
     1501 cggtcgagag cggcgtgggg ccagccacac tgccgtacgt gggcttcggc gtcgtgcttg
     1561 ccgactacga caacgacggc gatctggatc tcgcgatcgt caacggacac gtcatcgaca
     1621 acgtcgcgct cgtgcgcagc ggcgcgaccc acgcgcaggc acgcctgctg ctgcagaacg
     1681 gtggcacggg gcgcttcacg gacgtgagcg cgcagtcggg ccccgccttc ggcgtcagcg
     1741 ccgtcggccg cgggctcgcc gcgggcgaca tcgacaacga cggcgatctc gatctgctgg
     1801 ccacgagcaa cggaggcccg gccgcgctgc tgcgcaacga cggcggcaac cggagcaacg
     1861 cgctgctcgt ccggctcaca ggaacgatga gcaaccgcga cggcatcggc gcgcgacttc
     1921 gcgcgaccac gggcgcgcgc acgcagatga gggagttgac ctcggggtcg agctacctca
     1981 gtcagaacga cgcgcgcgta catctaggtc ttggcagcgc cgccatggtc gaccggctgg
     2041 agattcgatg gccgagcggc cggacggaga cccttgagaa ggtcagcgcg aatcagatca
     2101 tcacggttcg cgagagggaa ggtatcgtcg gcacagcccc gctggcgaga taaacccggt
     2161 ccggcttaag ccggacacta cgtacttccg cttcttctga tctgtcacgc agcctctctg
     2221 aatccgccgc agtgttaaga aaacgttaag gtgtaaaatc cgccctgaca tgacagtgat
     2281 gtcgacggtc cgccggggtc cgaaaacgtt gacgactgct gcgttcctgg tcgccgccct
     2341 gacagtcctg cccgcgggcg ctgcggccca gagagatccg ttcatcgacg cgttcgtcag
     2401 ctttcactcg gcgctgtcgg gaacctatgg agacgaagga ccgaaggtcg cagcgtcgct
     2461 ggaccagatg gccgcgagcc tcgccgcgtg ggatcggaca gccgccggcg ccgtcgcgaa
     2521 gctgacgtcg cagcgaacca acacggccgg cgatctcgcg ctcgcgtatc tcgactgggg
     2581 ccggctcgac gaggctctcg cggcactcga ttcggcaatc gcgatcgagc ccacgcgcgc
     2641 cgccttccac cagcttcgag gcctgatcct cgacgcgtca ggtcgccgcg acgatgccat
     2701 cgcggcgttc gcttcagcgt gggcgctcga cccgaacgac ccgattaaag cctacctcct
     2761 ggccgaccgg cgcggcaccg atgcgggcgc gggcgatctt caaccgcagc tggcggccct
     2821 gctcgcatcc tacgggcgag gcacgcccgc cgggcgcgcg ccgttcattc cgctgacgct
     2881 catcgaagac cgcgccgcca cgaccccgat cttttcgcca gccccctacg ccggcggctt
     2941 cgcgctcgtg gcgcagggcc gttaccagga cgcgatcgcc cgcttccggg aggcgatggc
     3001 gggcgacggc ctcgtggccg acacgccggc tcgatccgag ccgatgtcga aggggatcgc
     3061 cgcgctgagg gagggccgcg tcggtgcggc catcgagctg ctcgaggccg cggtggcggc
     3121 ggcgccgcgg tcgtcggagg cccaccgcat tcttggcgcc gcctacggag ccaatcgaaa
     3181 tcacgacaaa agcgtggcgc atctcaacac cgcgatcggc ctcgctcccc gcgacgaacg
     3241 cgcgcgcgtc acgctgggcc gcgagctggc cgacgccggc acgttcgagg aagcggaacg
     3301 cgcgcttcga gacacgatcg cgatgctgcc agcctcgggc gaggcgcgtt gggcgctcgc
     3361 caatctctac gacaagaacg gacgcggccc cgacgccgtt cgggagctcg aggcggccgc
     3421 gtcgctcacg gtgatggcgg gcaagggagc gatctactgg cggatcgccg aaatctccca
     3481 cgtccatcag gactacgaac gagtcgccgg cgcgctgtcg cgccgcgcga ggctcatgcc
     3541 caacgagccg atcgcgcaca aggatctcgg cctcgcctac aaccggatgg gccggcagga
     3601 tcaggcgctc atcgagctcg tcatggcctc gctccttggc ctcgaggacg cggaatcgct
     3661 cggggtcatg ggccagcttc atctcaacgc cggccgctac gccacggcgg aggcagtgtt
     3721 gcggcgcgcc gtcgcgctgc agcccgatgc cgcacagccg cggtacgccc tcggcagcac
     3781 gctgctccgc ctcgggaaag ccgaggaagc gaaatcgcag ctcgcggagt tccagcgcct
     3841 gcgcgccgcg gcgctcgacg agcagcgccg cac
//
LOCUS       DBNGSH010000889         2836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3845345, whole genome shotgun sequence.
ACCESSION   DBNGSH010000889 DBNGSH010000000
VERSION     DBNGSH010000889.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3845345"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1062)
                     /locus_tag="AC16CY_20450"
     CDS             complement(145..1062)
                     /locus_tag="AC16CY_20450"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3356234.1"
                     /translation="MIGVLISRLRLGAGLCLVLAGAVPLEAKDPCVAWPVAHTASQTT
                     AVQTAKDPDTFAVASLNMAGQSRIADVLMEWTHQRTLDVLLLQEVGEKSNDGARFAAD
                     LAERLGFHVAYAPANRLGGTESTQGLAIVSRYPLADVRVDLLKYKALRFKAKCRIALS
                     ATVATTSGPVRLVNVHLDTRINSEDRVDQLAPVLEGLKGATGPQIIGGDMNTANIRWF
                     QTMYPLPFFQRQVAAVRALFTKNGFDSPFFASTPSTFKFLGFPLKLDWLYLKHLKPVE
                     YDVDHVRFSDHRGIWAHVKSDPVASVNVQ"
     gene            complement(1059..2387)
                     /locus_tag="AC16CY_20455"
     CDS             complement(1059..2387)
                     /locus_tag="AC16CY_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3356235.1"
                     /translation="MTSTSDRLTPVQWLICAIAALGFAFDSYELLMLPLIVRPALADL
                     LGVPGNSLLVNEWVGTMLYVPAVAGGIFGLLGGYLTDLFGRRRVLVWSILLYAFSAMA
                     AGYSTSVYQLLFWRCCTFIGVCVEFVAAVAWLAELFADPVRRERIIGYTQAFGSFGGL
                     MVTGAYYLIVTYEESFPLIRGEQIAWRYTLISGVIPAIPLILIRPFLPESPAWQQKKK
                     AGTLKRPSFAALFTPEFRQTTIVTSVMMACSFAAAFGAIQQMPRVVPGIAEVRTMAPT
                     AQEQTIAGVQAFQEYGGLTGRILLAALATIIIGRRRLLYVFQIPGLVILPIVFLLAPT
                     GGLALAQWGIFFVGLTTVAQFSFWGNYLPRVYPTHLRGTGESFAANVGGRMIGTCAAL
                     VTTSLVPLMPGAAVPIKLAYAAAVVGTTVYVIGVIASRWLPEPKSTELPE"
     gene            complement(2452..>2836)
                     /locus_tag="AC16CY_20460"
     CDS             complement(2452..>2836)
                     /locus_tag="AC16CY_20460"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3356236.1"
                     /translation="GRRLRPSPQFDAMPPDFRELGPSYRASNPEGTQRWIELERASRP
                     EGLRPPPLTARNRVTFAALETLKVPTLLLTGDADLYTPPPVLQLVARRIRGAESLVID
                     GAGHSAYWEQPEAFNRAVLAFIGKH"
BASE COUNT          545 a          913 c          961 g          417 t
ORIGIN      
        1 ggatcggccg cgagacggtc acctacgtga gcaacatcta caagtactac gtcgcctaca
       61 agctcatcgc gaccgagaac acgcgacgga aggccgcaaa ggaagcgatc gagaaaagca
      121 gccagtaggc cgcggcggct gccgctattg cacgttcacg gacgcaacgg ggtctgattt
      181 cacgtgcgcc cagatccccc ggtggtccga gaaccgcacg tggtccacgt cgtactcgac
      241 cggcttcagg tgcttcaggt acagccagtc cagcttcaac ggaaagccca ggaacttgaa
      301 ggtcgagggc gtgctcgcga agaagggcga gtcgaagccg ttcttggtga acagcgcgcg
      361 caccgcggcg acctgacgct ggaagaacgg cagcgggtac atcgtctgga accagcggat
      421 attcgcggta ttcatgtctc caccgatgat ctgcggccct gtggcccctt tgagcccctc
      481 gagcacgggc gccagctgat ccacccggtc ttcgctgttg atccgtgtat ccaggtgcac
      541 gttgaccaga cgcaccgggc ccgacgttgt cgccaccgtc gcgctgaggg cgatccggca
      601 cttcgccttg aagcggaggg ccttgtactt cagcaagtca acgcggacgt cggcgagcgg
      661 atagcggctg acgatcgcga ggccctgcgt ggactccgtg ccgccaaggc gattggccgg
      721 cgcgtaggcg acatggaagc ccaggcgctc agcgagatcg gccgcgaagc gcgcgccgtc
      781 gtttgacttc tccccgactt cctggagcag gaggacgtcg agcgttcgct ggtgggtcca
      841 ctccatcagc acgtcggcga tccggctctg ccccgccatg ttcaggctgg caacggcgaa
      901 ggtgtccggg tccttcgccg tctggacggc cgtcgtctgg ctggccgtgt gtgcgacggg
      961 ccatgcgacg cacggatctt tcgcctcgag gggcaccgcg ccggcgagca cgaggcagag
     1021 ccccgcgccc agacggagac gtgagatgag aacaccgatc attcgggcag ctccgtggac
     1081 ttgggttccg gaagccagcg gctggcgatg acgccgatga cgtagaccgt cgtgcccacc
     1141 accgccgcgg cataggccag cttgatcgga accgccgcac ccggcatcaa cgggacgagc
     1201 gaggtggtga cgagcgccgc gcacgttccg atcatccggc cgccgacgtt ggcggcaaag
     1261 ctctcacccg tgccccgcag gtgcgtcggg tagacccgtg gcaggtagtt gccccagaag
     1321 ctgaactgcg cgaccgtcgt caagccgaca aagaagatac cccattgcgc cagcgcgaga
     1381 cccccggtgg gcgcgagaag gaagacgatc ggcaggatca cgagaccggg aatctggaag
     1441 acgtagagca gccggcggcg cccgatgatg atggtggcca gcgccgcgag caggatccgg
     1501 ccggtcagcc cgccgtattc ctgaaaagcc tgaacgccgg caatcgtctg ttcctgcgcg
     1561 gtcggcgcca tcgtccgcac ttccgcaatg ccgggaacga cgcgcggcat ctgctggatg
     1621 gcgccgaacg cggcggcaaa ggagcacgcc atcatcaccg aggtgacgat ggtcgtctgc
     1681 cggaactccg gcgtgaacag ggcggcgaag ctggggcgct tcagcgtgcc cgccttcttc
     1741 ttctgctgcc aggcgggcga ttcaggcagg aacgggcgga tgagaatgag cgggatcgcg
     1801 ggaatcactc cggaaatcag cgtgtagcgc cacgcgatct gctcaccccg aatcagcggg
     1861 aacgactcct cgtaggtcac gatcaggtag taggctcccg tgaccatgag gccgccgaac
     1921 gagccgaatg cctgcgtgta gccgatgatg cgctcgcgcc gcacgggatc ggcgaagagc
     1981 tccgccagcc aggccaccgc cgcgacaaac tcgacacaga cgccgatgaa cgtgcagcag
     2041 cgccagaaca gcagctggta caccgatgtg gagtacccgg cggccatcgc cgagaaggcg
     2101 tagagaagaa tgctccacac caggacgcgg cggcgcccga agagatcggt gaggtagcct
     2161 ccgagcagtc cgaagatccc gccggccacg gccggcacgt acagcatcgt gccgacccac
     2221 tcgttcacca gcaggctgtt cccgggaacg ccgagcagat cggcgagcgc ggggcggacg
     2281 atgagcggca gcatcagcag ctcgtaggag tcgaacgcga acccgagcgc ggcgatcgcg
     2341 cagatcagcc actgcaccgg ggtcaaacgg tctgaagtcg atgtcatgag gagtcagggg
     2401 attctactcg actgcatcac ggtccggctg aagccggaca ctacgtacgc atcagtgctt
     2461 cccaatgaag gcgagcacgg cgcggttgaa ggcctccggc tgctcccagt aggcggagtg
     2521 tccagcgccg tcgatgacga gcgattcggc gcccctgatc cgcctggcga cgagctgcag
     2581 caccggcgga ggcgtgtaga gatcggcatc gccggtgagc agcagcgttg gcactttcag
     2641 cgtctccagc gccgcgaacg tcacgcggtt tcgtgccgtc aacggcggag gccgcaaccc
     2701 ttcgggacgg ctggcgcgtt cgagctcgat ccagcgctgc gtgccctccg ggttcgacgc
     2761 gcggtacgac ggaccgagct ctctgaaatc aggcggcatc gcgtcgaact gaggcgaggg
     2821 cctgagccgc cggccg
//
LOCUS       DBNGSH010000890         4166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_791037, whole genome shotgun sequence.
ACCESSION   DBNGSH010000890 DBNGSH010000000
VERSION     DBNGSH010000890.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_791037"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC16CY_20465"
     CDS             <1..278
                     /locus_tag="AC16CY_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356237.1"
                     /translation="TVRIKIDDDPELVQQWSDSESGQELFAPDGIALARRLADAEQMQ
                     FSFTPYNAGAATASFAVQGAGELVGLVANTCGWRLDGAAAPARRAAR"
     gene            complement(275..2899)
                     /locus_tag="AC16CY_20470"
     CDS             complement(275..2899)
                     /locus_tag="AC16CY_20470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3356238.1"
                     /translation="MIGTRIAHYEINSHLGSGGMGEVYEATDSKLGRRVAIKVLPEEF
                     ARDADRVARFEREARVLASLNHPHIAAIHGFDESGGRNFLVMELVEGETLADLIARGA
                     LSVDEALPIALQITEALEAAHEKGFIHRDLKPANIKVTSEGQVKLLDFGLAKAFEAQP
                     SEIDVSNSPTLSAMATHAGVILGTAAYMSPEQARGAAVDRRTDIFAFGSVLFEMLTGR
                     HAFEGENVADILAAVLKVEPDWSRLPADVPPTIRRLLRRCLNKDRRRRPQAAGDVRIE
                     IEEANAEPDTTAAGHAAPPSRRGERAAWIIAALAASAALVLATLAFRPLPEAPEMRVE
                     VTTPPTPDPISIAVSPDGRRLVFVASGDGVPRLWLRALDTAALQPLAGTEGASYPFWS
                     PDSRSIGFFAAGQLKRIEVAGGLPRRLADAAEGRGGTWSVEDVIVFSPQPGSRLQRVS
                     ASGGDAAVVDHITVDSARFPQFLPDGRRFLFFVIGGFGRATLKGVYLSSLDGSEPRHL
                     TDADASGLYMPPGWLLYVRGSALMARPFDEARGEFEGEPVVVADPVGVDANVYAAAVS
                     VSTAGLVAYRSGGPSRRQLAWFDRFGRPSGTVGQPDENDLLGPELSSDGRRVAVFRSI
                     RSTRNVWLLDVDRGVSNPFTDEPSSAQYPRWSPDGGRIAFQSLRTERPGVYLKPLTGT
                     AGEELLVAMPLTIPNDWSSDSRFLVYTTTDPKTRGDLWTLPLGGDRKPAPFAVGNDDE
                     SSAQFSPDGRWIAYRSDESGRYEIYVQPFPGPGERTRVSIDGGTEPRWRADGREIFYI
                     SPDAKMMAVPFQSPGPAPDIGKPVPLFQTRKVRGGTSNVLQQYDVSRDGRFLINVNAD
                     ESVASPIMLIMNWKRPAD"
     gene            complement(2916..>4166)
                     /locus_tag="AC16CY_20475"
     CDS             complement(2916..>4166)
                     /locus_tag="AC16CY_20475"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3356239.1"
                     /translation="DKLILCCGRVNRGFAIRGDRLYMTTLDAHLLALDTRTGNVIWDV
                     EIDDPARGYSATVAPLVVKDKIIVGIAGAEYGVRGFIDAFDADTGKKAWRFWTVPEPG
                     QPGGDTWTGDSWRRGGGSTWVTGAYDPELNLVYWGTGNPAPDSYGAARLGDNLYTDAI
                     VALDADTGRLRWHYQFTPHDVHDWDAVQVPILADLAIGGTRRKVVMTANRNGFFYTLD
                     RTSGELLRARPYIKTTWAEEVGRDGRPILLPANQIGPEGTITCPDVGGGTNWMSPSFN
                     PQTGLFYVTTREVCAKYYAFPQKFLEGHVYWGGAVERIPGEPGYGALRAIDPVSGLVR
                     WEFKYPKPSMGGTLSTASGVVFAGEADGNLMAFDAVSGKHLWHLQTGAGVRSGPMTFM
                     LNNRQYIVVASGSTVIALALPAGR"
BASE COUNT          683 a         1424 c         1377 g          682 t
ORIGIN      
        1 ataccgtgcg catcaagatc gacgacgacc ccgaactggt gcagcagtgg tcggattccg
       61 agagcggcca ggagctcttc gcgcccgacg gcatcgcgct cgcgcggcga ctggcagatg
      121 ccgagcaaat gcagttcagc ttcacgccgt acaacgccgg ggccgcgacc gcgagcttcg
      181 ccgtgcaggg cgccggcgag ctcgtcggac tcgtcgcgaa cacctgcggg tggcgcctcg
      241 atggtgccgc cgctcccgcc agacgcgcgg cccgttagtc ggccggccgc ttccaattca
      301 tgatgagcat gatgggcgac gcgacgctct cgtccgcgtt gacgttgatc agaaaccgtc
      361 cgtcccgcga cacgtcgtac tgctgcagca cgttgctcgt gccgccccgg accttcctcg
      421 tctgaaagag cggcacgggt ttgccaatgt cgggcgccgg gcccggggat tggaacggca
      481 ccgccatcat cttcgcgtcc ggcgagatgt agaagatttc tctcccgtcg gcgcgccagc
      541 gcggttccgt gcccccgtcg atcgataccc gggttctctc gccggggccg gggaacggct
      601 gcacgtagat ctcgtatcgc ccggactcgt cggaccggta cgcgatccag cgcccatcgg
      661 gggaaaactg cgcgctcgac tcgtcgtcgt ttccgaccgc gaagggcgcc ggcttgcgat
      721 cgccgccgag cggcagcgtc caaaggtcgc cgcgcgtctt cggatcggtg gtcgtgtaga
      781 cgaggaagcg gctgtcggaa gaccagtcgt ttggaatcgt aaggggcatc gccacgagca
      841 gctcttcgcc ggccgttcct gtcagcggct tgaggtacac gccgggccgt tccgtccgca
      901 atgactggaa cgcgatgcgg ccaccgtctg gtgaccatcg cgggtactgc gcgctcgacg
      961 gctcgtccgt gaaagggttc gacaccccgc ggtcaacgtc gagcagccag acattgcgcg
     1021 tgcttctaat cgaacgaaac accgccacgc ggcgtccgtc cgaggagagt tctggcccga
     1081 gcaggtcgtt ttcgtctggc tgtccgactg tgcccgaagg acggccgaaa cgatcgaacc
     1141 acgccagctg ccgcctgctc ggtccgccgc ttctgtacgc caccaggcct gccgtcgaaa
     1201 ccgacacggc ggcagcatag acattggcgt ccacgccgac tggatccgca acgaccaccg
     1261 gctccccttc gaactcgcca cgcgcttcgt cgaagggccg ggccatcagg gcgcttcccc
     1321 ggacgtagag cagccagccg ggcggcatat acaggcccga cgcgtccgcg tctgtgagat
     1381 gccgcggctc cgatccgtcg agcgaactga gatagacacc cttgagcgtt gcccggccga
     1441 agcccccgat gacgaagaag agaaaacgcc ggccgtccgg gaggaactgg gggaaccggg
     1501 ccgagtcgac ggtgatgtga tcgacgacgg ctgcatcgcc cccggaggcc gagacacgtt
     1561 gaagacgcga tccgggctgt ggcgagaaca cgatgacgtc ctcgacactc cacgtaccgc
     1621 cccgcccctc ggcggcgtcg gcaagcctac gcggcagacc gccggcgacc tcgatccgct
     1681 tcagctggcc ggcggcaaag aagccgatcg atcggctatc cggcgaccag aacgggtagc
     1741 ttgccccctc ggttccggcc agcggctgga gggcggccgt gtcgagcgcg cgcagccaca
     1801 ggcgcggaac cccgtcgccc gacgcgacga agacgagccg tcgtccgtcc ggcgagaccg
     1861 cgatcgagat cgggtccggc gtcgggggcg tcgtcacctc cacgcgcatc tcaggggcct
     1921 cgggcagcgg acgaaaggcc agcgtcgcca gaacgagggc cgccgacgcc gcgagagccg
     1981 caatgatcca agccgcacgt tcgccgcgcc tcgatggagg agccgcgtga ccggcggccg
     2041 tcgtgtccgg ttcggcgttt gcctcctcga tctcgattcg aacatcgccc gcggcctgcg
     2101 gccgccggcg ccgatccttg ttcaagcacc gccgcagcag ccgccggatc gtcgggggaa
     2161 cgtctgcggg caagcggctc cagtccggct ccactttgag caccgccgcc aggatgtccg
     2221 cgacgttctc tccctcgaac gcgtggcgcc cggtcagcat ctcgaagagc accgagccga
     2281 aagcgaagat atccgtccgc ctgtccacgg ccgcgcctct ggcttgttcc ggcgacatgt
     2341 aggcggcggt ccccagaatc acgccggcgt gtgtagccat ggcgctgagc gtcggcgagt
     2401 tcgagacgtc gatttccgac ggctgtgctt cgaaggcctt cgccaggccg aagtcgagca
     2461 gctttacctg gccctcggag gtgaccttga tgttggccgg tttcaaatcg cggtgaatga
     2521 atcctttttc gtgggctgcc tcgagtgcct ccgtgatctg cagcgcaatc ggcagtgcct
     2581 catccacgga cagagcgccg cgggcgatga gatccgccag cgtctctcct tccacgagct
     2641 ccatgacgag gaaattgcgg ccgcccgatt cgtcgaatcc atgaatcgct gcaatgtgag
     2701 gatgattcag cgaggcaagc acacgtgcct cgcgctcgaa tcgcgccact cggtccgcgt
     2761 cgcgcgcgaa ctcctcgggc aggaccttga tcgcgacgcg cctcccgagc ttggagtcgg
     2821 tggcttcgta cacctccccc atgcctcccg accccaggtg cgagttgatc tcgtaatgcg
     2881 cgatgcgcgt gccgatcatc ggaggcgcgt ggcggctagc ggcccgcggg cagcgccagc
     2941 gcgatcacgg tcgatccaga cgccacgacg atgtactgcc ggttgttcag catgaaggtc
     3001 atcgggcccg agcggacccc ggcgcccgtc tgcagatgcc acagatgctt gccgctaacg
     3061 gcgtcgaacg ccatcaggtt gccgtccgct tcgcctgcga agaccacgcc cgaggccgtg
     3121 ctcaacgtgc cgcccatcga cggcttcggg tacttgaact cccagcgcac gaggccgctg
     3181 acgggatcga tcgcgcgcag cgcgccgtag ccgggctcgc cgggaatgcg ctcgaccgct
     3241 ccgccccagt acacgtgtcc ctcgaggaac ttctgcggga aggcgtagta cttcgcgcag
     3301 acctcgcgcg tcgtgacgta gaacagtccg gtctgcgggt tgaaggatgg cgacatccag
     3361 ttcgttccgc cgcccacgtc aggacaggtg atcgttccct cggggccgat ctggttcgcc
     3421 ggcagcagaa tgggacggcc gtcgcgcccg acttcctccg cccacgtggt cttgatgtag
     3481 gggcgcgcac gaagcagttc gccgctggtc cgatcgagcg tgtagaagaa gccgttccgg
     3541 ttggcggtca tcacgacctt gcgccgcgtg ccgccaatgg ccaggtccgc caggatcggc
     3601 acctgcaccg cgtcccagtc gtgaacgtcg tgcggtgtga actgatagtg ccagcgcaag
     3661 cgccccgtgt cggcatcgag cgcgacgatc gcgtcggtgt agaggttgtc tccgaggcgg
     3721 gcggcgccgt acgaatcggg cgcggggtta cccgttcccc agtagacgag gttcagctcg
     3781 ggatcgtagg cgccggtcac ccacgtcgag ccgccgccgc gtcgccagga gtcgccggtc
     3841 caggtatcgc cgccaggctg gcctggttcg ggaacagtcc agaatcgcca tgctttcttc
     3901 ccggtgtcgg cgtcgaacgc gtcgatgaac ccgcggacgc cgtactcggc gcctgcgatc
     3961 ccgacgatga tcttgtcctt gacgacgagc ggcgccacgg tggccgagta cccgcgggca
     4021 ggatcgtcaa tctcgacatc ccagatgacg tttccggtcc tggtgtcgag cgccagcaga
     4081 tgcgcgtcga gcgtcgtcat gtagaggcga tcgcctcgga tcgcaaagcc gcggttgacg
     4141 cggccgcagc agagaatcag cttgtc
//
LOCUS       DBNGSH010000891         4244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1639613, whole genome shotgun sequence.
ACCESSION   DBNGSH010000891 DBNGSH010000000
VERSION     DBNGSH010000891.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1639613"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="AC16CY_20480"
     CDS             complement(<1..1053)
                     /locus_tag="AC16CY_20480"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HIR3356240.1"
                     /translation="MRVRITFAVCAALVIATTSGVPAAISDPVRTDAGLVSGVTLASG
                     VRAFKGIPFGAPPVGELRWKEPQQVATWDGVRKADRFGNVCVQPSQPARSPNNVSVDL
                     PDSPKPSEDCLFLNVWTSAAAANARQPVMVWIFGGAYSEGAGSSPHNDGEALATKGAV
                     LVTFNYRLGAFGFFSHPELTKESGRNASGNQGLMDTIATLRWVQRNIAAFGGDPNNVT
                     IFGESAGAAMVGGLVGSPVAKGLFHRAISQSGQWMGLGIGAMAPPAPRPLPSLAELRA
                     RSTEEVVKTLRGSGMIIDGWIVPEDLSITFANGRQNAVDLIAGSNKDEHTSMGGNVAF
                     RDTLAWGMRLFAERQAA"
     gene            complement(1100..3775)
                     /locus_tag="AC16CY_20485"
     CDS             complement(1100..3775)
                     /locus_tag="AC16CY_20485"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3356241.1"
                     /translation="MSQLPPGTTIGAFQIAAHIGSGGMGEVYRARDTKLKRDVALKVL
                     PPAFALDPDRLARFRREAQVLASLNHPNIAAIYGLEEAAVIALVLELVNGPTLADRIA
                     EGPIPLDEAQSIARQIAKALEAAHEHGTVHRDLKPANIKLRPDGAVKLLDFGLAKALD
                     QTPQDGAQGAEDPDNSPTITTPAMTRAGVILGTAAYMSPEQARGRPVDKRCDLWAFGC
                     VFYEMLTGRRAFQSEDVADTLATVLKSEPDWNAWPANVPASIRVFVRRCLEKDPKRRI
                     ADATTALFVIDDLATTSAGVTHQTKRPEWRVAAYIALTAVLAAGAGAYIVREPRDGRV
                     YRSTIPVTVTSDAGVEATLSLSPDGRRLAFIGSDANGARLVYVRPLDGLAPQPLAGTE
                     GAAALFWSPDSRFLGFVTSNKLKTIDTTGSAPPFEVADASGTRPGAWNGDDVILFTPT
                     QGSPLFRVSATGGMPSAVTSLDTKAGESSHAFPFFLPDGRHFLYLAQGSGGVPRGVYA
                     GSLDSTDRTRIFDSASNVQFARGALVFLRGSTLMAQPFDAGRMALSGDAVPLADHVQV
                     NEGTDFLRTGTFSVSEAGHLVYRADTSGGSELVWRDRTGTETGKLGDRAKYLDVGLSP
                     DAAHATVSIMEAGTTTRDVWTYEVAKGLRNRLTFDREDDLDARFSPDGSRIVFSSRRK
                     QGHLDLYIKAASGAGTEEELLVDDMDKYPQSWSPDGRFLLYVGTSPTTGQDLWVLPLT
                     GDDRKPFPFLNTEASEGTGVFSPDGRWIAYRSNQTGGRFEVYVASFRPGGGGGGQRQI
                     STTGGSAPRWPRDSKELVYVGPANTLMSAAVSVEAERVDVGAVKKLFQVHPVTPRYFY
                     DVSPDGQRFLVNTADPSSAAAPLMLVVNWPALLQK"
     gene            complement(3831..>4244)
                     /locus_tag="AC16CY_20490"
     CDS             complement(3831..>4244)
                     /locus_tag="AC16CY_20490"
                     /inference="COORDINATES: protein motif:HMM:NF019157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR3356242.1"
                     /translation="GGILSATSSWTNLNTYSNHGGKLVFFHGVSDPWFSALDTVDYFE
                     RMTKANGGPEKVTNWGRLYLVPGMGHCAGGRALDSFDLLTAVVEWVEKGTPPAVTATG
                     RTLPGRSRPLCAYPQHPHYKGQGDPQDAKSFECRD"
BASE COUNT          727 a         1427 c         1335 g          755 t
ORIGIN      
        1 cgccgcctgg cgctccgcga acaaacgcat cccccaggcc aacgtgtcgc ggaaggccac
       61 gttgccgccc atggacgtgt gttcgtcctt gttggatccc gcgatgagat ccaccgcgtt
      121 ctgcctgccg ttggcaaacg tgatcgagag atcctccgga acaatccagc catcgatgat
      181 catcccggag ccgcgcagcg tcttgacgac ctcttcggtg gaccgtgcgc gcagctcggc
      241 gagcgaagga agaggacgtg gcgccggcgg cgccatcgcg ccgataccga gccccatcca
      301 ctggccgctc tgggaaatcg cgcgatggaa cagccctttg gcgaccggcg atccgacgag
      361 gccgcccacc atggccgcgc ccgctgattc gccgaagatg gtgacgttgt tcggatcacc
      421 accgaaggcg gcgatgttcc tctggaccca gcgcagcgtg gcgatagtgt ccatcagtcc
      481 ctggttgccg gacgcgtttc gtcccgactc cttcgtcagc tccgggtgcg agaagaatcc
      541 aaacgctccg agcctgtagt tgaacgtgac gagcacggcg cccttcgtgg ccagcgcctc
      601 gccgtcgttg tgcggactcg atccggcgcc ttcactgtac gcgccgccaa aaatccagac
      661 catcaccggc tggcgcgcat tggcggcggc cgccgacgtc cagacattga ggaacaggca
      721 gtcctcgctc ggcttcggcg aatccggcaa gtccaccgag acgttgttcg gcgagcgtgc
      781 cggctgcgac ggctgcacgc agacgttgcc gaaccgatcg gccttccgga caccatccca
      841 cgtcgcgacc tgctgcggct ccttccatcg cagctcgccg acaggcgggg ccccgaacgg
      901 aatccccttg aaggctcgca cgccggacgc cagggtcaca cccgatacga ggcctgcgtc
      961 ggtcctgaca ggatcggaga tggctgccgg aacgccgctt gtcgtggcga taacgagcgc
     1021 ggcgcagact gcgaacgtga ttctgaccct cattgtgcgg ttaccctctc cctcttctgg
     1081 ccgcgcctgt tgcggtcctc tacttctgca ggagcgctgg ccaattgacg acgagcatga
     1141 gcggcgccgc cgcagacgac gggtccgcgg tgttcacgag aaaccgctgg ccgtctggcg
     1201 agacgtcgta aaagtagcgc ggcgtcacgg gatgtacctg aaacagtttc ttgacggcac
     1261 caacgtcaac gcgctcggct tccaccgata cggctgccga catcagcgtg ttggccggcc
     1321 cgacgtaaac cagctctttg ctgtcccggg gccaccgagg agcgctgccg cccgtcgtgg
     1381 aaatctgcct ttggccccct ccaccccctc caggtcgaaa cgaggcgacg tacacttcga
     1441 atcggccccc tgtctggttc gatcggtagg caatccagcg gccgtctggc gaaaacacgc
     1501 cggtgccttc actggcctcg gtgttgagaa aaggaaaggg ctttcgatcg tctccggtca
     1561 gcggcaacac ccacaggtcc tgtcccgtgg tcgggctagt cccgacatag agaagaaacc
     1621 gcccgtcagg cgaccagctt tgcggatact tgtccatgtc gtcaaccagt agctcctcct
     1681 cggtgccggc gccgctggcc gccttgatgt agagatccag gtgtccctgc ttgcggcgtg
     1741 agctgaagac gatgcggctg ccatcgggcg aaaaccgcgc gtccaggtcg tcttcccgat
     1801 cgaatgtgag tctgttgcga agccccttgg cgacttcgta ggtccacaca tcgcgagtcg
     1861 tggttccagc ctccatgatg ctcacggtcg cgtgagcggc gtcgggagaa agaccgacat
     1921 cgagatactt cgcgcggtcg ccgagcttgc cagtctcggt gcctgtgcgg tcccgccaca
     1981 ccagctccga gcctcctgac gtgtcagccc ggtagacgag gtggcccgct tcagaaacac
     2041 tgaacgtgcc cgttcggaga aagtcggtcc cttcgttgac ctggacgtga tccgcaagcg
     2101 gcaccgcatc tcccgagagc gccatgcggc cggcgtcgaa gggctgcgcc atcagcgtgc
     2161 tgccacgcag aaacaccagc gcaccccgtg caaactgcac gttggacgcg ctatcgaaga
     2221 tccgtgtccg atccgtcgag tccagcgagc cggcatacac gccgcgaggc actccgccgc
     2281 tgccctgggc cagatacagg aagtgccggc catcaggcag gaagaacggg aacgcatgag
     2341 agctctcccc agctttcgtg tcgagcgacg tgaccgcaga cggcatgccc cccgtcgctg
     2401 agactcgaaa cagaggcgat ccctgcgtcg gcgtgaaaag aatgacgtcg tcgccgttcc
     2461 aggcgcctgg ccgcgttcca ctggcgtcag cgacctcgaa ggggggcgca cttccggtcg
     2521 tgtcgatcgt cttgagctta ttactggtga cgaacccgag aaagcggctg tccggtgacc
     2581 aaaaaagcgc cgcggcgccc tcggtcccgg cgagcggctg cggcgcgagg ccatcgagtg
     2641 ggcgcacgta aaccagtcgg gcgccgttgg cgtccgaccc gatgaatgcc agccggcgtc
     2701 catcgggcga aagcgacagc gtcgcttcaa cgcctgcatc ggacgtgacg gttaccggaa
     2761 tggtgctgcg atagacgcgc ccgtcgcgag gctctcgcac gatgtaggcc cctgctccgg
     2821 cggcgagcac ggcggtgagc gcgatgtagg ccgcgaccct ccactcgggc cgcttcgtct
     2881 ggtgagtcac gccggcggac gtcgtcgcga ggtcgtcgat cacgaagagt gcggttgtgg
     2941 catccgcgat tcgccgtttg ggatcctttt ccaggcatcg ccgaacgaac acgcgaatcg
     3001 acgccggtac gttcgcaggc catgcgttcc agtcgggctc gctcttgagc acggtcgcga
     3061 gcgtgtcggc cacgtcctcg ctctggaacg cacgcctccc ggtcaacatc tcgtagaaca
     3121 cgcacccaaa ggcccagaga tcgcagcgct tgtcgaccgg tcgtcctctg gcctgttcgg
     3181 gactcatgta cgcggctgtc ccgagaatca cgccggccct ggtcatcgcg ggagtcgtga
     3241 tagtcggtga gttgtcggga tcttcggcgc cctgagcccc gtcctgcgga gtctggtcca
     3301 gcgctttcgc gagtccgaaa tccagtaact tcacggcacc atccgggcgc agcttgatgt
     3361 tcgcgggctt caggtcgcgg tggacggtgc cgtgctcgtg cgccgcctcc agcgctttgg
     3421 cgatctgcct cgcgatcgac tgcgcctcgt ccaggggaat cgggccctct gcaatgcggt
     3481 cggcgagcgt cggaccgttg accagttcca ggaccagcgc aatgacagcc gcctcttcga
     3541 ggccgtagat ggccgcgatg ttcggatgat tcagcgacgc gaggacctgc gcttcgcgcc
     3601 ggaaacgagc taaacgatcg gggtcgagcg cgaaggccgg tgggagaacc ttcagcgcga
     3661 cgtcccgctt cagcttcgta tcgcgggcgc gatagacctc gcccatgccg ccagatccga
     3721 tatgggccgc gatctggaac gctccaatcg tcgttcccgg cggaagttga ctcatgggcg
     3781 tgcctctatt gccgtcacgt ctgccttttg cccttagacc tactcggcgc tcaatcccga
     3841 cactcgaaac tcttcgcgtc ctgcggatca ccctgccctt tgtagtgcgg gtgctgcggg
     3901 tacgcgcaca acggacgact gcgtcccggc agcgtcctgc cggtcgccgt gacggcaggc
     3961 ggcgttccct tttcgaccca ctcgacgacc gcggtcagca gatcgaagct gtcgagggcc
     4021 cggccgcccg cgcaatgtcc catcccgggc acgagataca aacggcccca gttcgtcacc
     4081 ttctcgggcc cgccgttggc cttcgtcatg cgctcgaagt agtccacggt gtcgagcgcg
     4141 gagaaccacg ggtcgctcac gccgtggaag aacacgagct tgccgccatg gttcgaatag
     4201 gtattgaggt tcgtccagct cgacgtggcc gacaggatcc cacc
//
LOCUS       DBNGSH010000892         4086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2149509, whole genome shotgun sequence.
ACCESSION   DBNGSH010000892 DBNGSH010000000
VERSION     DBNGSH010000892.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2149509"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..2304)
                     /gene="fusA"
                     /locus_tag="AC16CY_20495"
     CDS             complement(196..2304)
                     /gene="fusA"
                     /locus_tag="AC16CY_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3356243.1"
                     /translation="MPRQTPLNRTRNIGIMAHIDAGKTTTTERILFYTGITYKIGEVH
                     EGTAVMDWMEQEQERGITITSAATTCFWRDMRINIIDTPGHVDFTAEVERSLRVLDGA
                     CAVFDAVSGVEPQSETVWRQADKYRVPRICFVNKMDRIGADFKATLSQIKTKLGANPV
                     AIQLPIGAEDKFTGVIDLVKMKAITYKDETMGADYDVSDIPAELMAEAQEYREKLIEK
                     VSEHDDKLLEKYLGGQEITESEIKAALRDRVIKSVYHEDTAFVVVICGTAFKNKGVQP
                     LLDAVVDYLPSPLDVPAISGLDPNAKEETLVERPASDDAPFSSLAFKLMTDPFVGQLT
                     FIRVYSGVLTSGSAVYNATKGKSERVGRLLKMHANKREEIKEVYAGDIAAAVGLKQVS
                     TGDTLCEEKKPVVLESMDFPEPVISLAIEPRTKGDQEKLGVGLQKLMSEDPTFRVNTD
                     EQTGQVVIAGMGELHLEIIVDRLKREFGVEASVGKPQVAYKETLTRAADGEMRYAKQT
                     GGRGQFAEVKIRLFPGEPGTGYIFENEIKGGTIPKEYIKPVDEGIKEALTRGILAGYP
                     IDDVRIELYDGKHHEVDSSEMAFKIAGSMAFQDAAKKAKPVLLEPVMRVEVVVPKDYM
                     GDVMGDLSGRRGRIQSQEDRGSTQIINARVPLSEMFGYATDLRSRTQGRATYSMHFDR
                     YEQTPQNVSEEVVARIQGTK"
     gene            complement(2343..2816)
                     /gene="rpsG"
                     /locus_tag="AC16CY_20500"
     CDS             complement(2343..2816)
                     /gene="rpsG"
                     /locus_tag="AC16CY_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR3356244.1"
                     /translation="MPRRREIPKRELAADPLYNSALVSKFINTVMHDGKRSTAERILY
                     QSFDIIKERTGDDPLKVFKKALDNVKPSLEVKSRRVGGSNYQVPVEVNPNRRLSLSIR
                     WLVGYARDRGDGKTMQEKLANELLDASNLRGGAVKKREDTHRMAEANKAFAHYRW"
     gene            complement(2884..3258)
                     /gene="rpsL"
                     /locus_tag="AC16CY_20505"
     CDS             complement(2884..3258)
                     /gene="rpsL"
                     /locus_tag="AC16CY_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR3356245.1"
                     /translation="MPTISQLVRQGRTKIVTKTKSPALQVSPQKRGVCVRVFTQTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGVGHNLQEHSLVLIRGGRVKDLPGVRYHVVRGTL
                     DAVGVQGRKQGRSKYGAKRPKQ"
     gene            3732..3893
                     /locus_tag="AC16CY_20510"
     CDS             3732..3893
                     /locus_tag="AC16CY_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356246.1"
                     /translation="MSVSPNAGMFANGSIPAGSVIHLRSHVPEEHRDDACDDHQSDGC
                     PMVERKSRL"
BASE COUNT          656 a         1338 c         1254 g          838 t
ORIGIN      
        1 gtcgtcttcc cgtggtcgat gtgcccgatc gtccccacgt tcacgtgcgg tttcgaacga
       61 tcaaactttt ctttcgccat ctgacgctcc gattcagaag gcctcagggc tcagacccca
      121 aggcctcagg gctgtctctg ttccaaggcc tcaggcctgg cccagccctg gggcccgagg
      181 cctgattccc ggggcctatt tcgttccctg tatcctggcc acgacctctt cgctcacatt
      241 ttgcggtgtc tgctcgtagc ggtcgaaatg catcgagtaa gtcgcgcggc cctgcgtgcg
      301 cgagcgcagg tcggtcgcat agccgaacat ctccgagagc ggcacgcgcg cgttgatgat
      361 ctgcgtgctt ccacgatcct cttgtgactg gatacgaccg cgacggcccg agagatcgcc
      421 catgacgtcg cccatgtagt ccttcggcac cacgacctcg acacgcatca cgggctcgag
      481 cagcacgggc ttggccttct tggccgcatc ctgaaacgcc atggagccgg caatcttgaa
      541 cgccatctcc gacgagtcga cttcgtggtg cttgccgtcg tacagctcga tcctgacatc
      601 gtcgatcgga tagccggcga ggataccgcg cgtgagcgct tctttgatgc cctcgtccac
      661 gggcttgatg tattccttcg ggatcgtgcc gcccttgatc tcgttctcga agatgtaacc
      721 cgtaccaggc tcgcccggga acaagcgaat cttcacctcg gcgaactggc cgcgaccacc
      781 ggtctgcttg gcgtaccgca tctcgccgtc ggcggcgcgc gtaagcgttt ccttgtacgc
      841 cacctgtggc ttgcctacac tcgcttcgac gccgaattcg cgcttcaagc ggtcgacgat
      901 gatctcgagg tgcagctcgc ccatgccggc gatgacgacc tggccggtct gctcgtcggt
      961 attgacccgg aaagtcgggt cttccgacat gagcttctgc aggccgacgc cgagcttctc
     1021 ctggtcgcct ttcgtcctcg gctcgatcgc gagcgagatg accggctcgg ggaagtccat
     1081 cgactcgagt acgacgggct tcttctcctc gcagagcgtg tcgccggtgc tgacctgctt
     1141 gaggccgacg gccgcagcga tatcgccggc gtagacctct ttgatctcct cacgcttgtt
     1201 ggcgtgcatc ttcagcaggc ggccgacgcg ctccgacttg cccttggtgg cgttgtagac
     1261 cgctgagccc gaggtcagta cacccgagta gacccggatg aacgtgagct ggcccacgaa
     1321 cgggtcggtc atgagcttga acgccagcga ggaaaagggc gcatcgtctg acgcggggcg
     1381 ctcgaccagc gtctcttcct tcgcgttcgg atcgaggccc gaaatggccg gcacatcgag
     1441 cggcgaaggc agatagtcca cgacagcgtc gagcagcggc tgcacgccct tgttcttgaa
     1501 cgcggtgccg cagatgacga cgacgaaggc cgtgtcctcg tggtagaccg acttgatcac
     1561 gcggtcgcgg agcgccgcct tgatctcgct ctcggtgatt tcctggccgc cgaggtactt
     1621 ctcgagcagc ttgtcgtcgt gctcgctgac cttctcgatc agtttctcgc ggtactcctg
     1681 cgcctcggcc atcagctcgg ccgggatgtc cgacacgtcg tagtcggcac ccatggtctc
     1741 gtccttgtag gtgatcgcct tcatcttgac gaggtcgatc acgcccgtga acttgtcttc
     1801 ggcaccgatg ggcagctgaa tcgcgaccgg attggccccg agcttcgtct tgatctggga
     1861 gagcgtcgcc ttgaagtccg cgccgatgcg gtccatcttg ttcacgaagc agatgcgcgg
     1921 cacgcgatac ttgtccgcct gacgccacac ggtctcggac tgcggttcga cgcccgagac
     1981 cgcgtcgaac acggcgcacg cgccgtcgag cacgcgcagc gagcgctcga cttccgccgt
     2041 gaagtcgacg tggccgggcg tgtcgatgat gttgatgcgc atgtcgcgcc agaagcaggt
     2101 cgtcgcggcg gacgtgatgg tgatcccgcg ctcctgttcc tgctccatcc agtccatgac
     2161 cgcggtgccc tcatgaacct cgccgatctt gtacgtaatc ccggtataga agaggatccg
     2221 ctcggtggtg gtcgtcttcc cggcatcgat gtgcgccatg atgccgatgt tccgggttcg
     2281 attcagcggt gtctgacggg gcatttctgg tccttggtgc ttggtgcttg gtgctgggtc
     2341 ccttaccagc ggtaatgggc gaaggccttg ttcgcttcgg ccatccggtg cgtgtcctcg
     2401 cgcttcttga cggcgccgcc gcgcaggttc gaggcgtcga gcaactcgtt ggccagcttt
     2461 tcctgcatcg tctttccgtc gccgcggtcg cgcgcgtagc cgaccagcca tcggatgctg
     2521 agcgacagcc ggcggttcgg gttgacttcg accggaacct ggtagttcga gccacccacg
     2581 cgccgcgact tcacctcgag gctcggcttg acgttgtcga gcgccttctt gaacaccttg
     2641 agcgggtcgt ctcccgttcg ctccttgatg atgtcgaagc tctggtagag gatgcgctcg
     2701 gcggtgctgc gcttgccgtc gtgcatcact gtgttgatga acttgctgac cagggcgctg
     2761 ttgtagagcg ggtcggccgc cagttctcgc ttcggtatct ctcgtctgcg tggcataact
     2821 ctgatccttg aaggtctcag ggcccggggc tcaggcctca ggcctcagca gggcccaggc
     2881 cgttcattgc ttcgggcgct tggcgccata cttcgagcgg ccctgcttac ggccctgcac
     2941 gcccacggcg tcgagcgtgc cgcggacgac gtgataccgc acgccgggca ggtccttgac
     3001 gcggccgccg cgaatgagca cgagcgagtg ctcctgaagg ttgtggccga cgccaggtat
     3061 ataggtcgtc acctcgatcc cgttggtcag gcgtacgcga gccactttcc gaagggccga
     3121 gttcggcttc ttcggcgtct gggtgaacac gcggacgcac acgccccgct tctgggggct
     3181 gacctggagc gcagggctct tcgtcttcgt cacgatcttc gtgcggccct ggcgtacgag
     3241 ctgacttatc gtcggcatac ggggtcggga gtcatttctt cgaggacacg cctactcggc
     3301 tggcccggga gcgtcctcga aaaatgactc ccgaccccat tttccgtaca cgcgcagccg
     3361 ccagccaagc agccccacgt ggttccaggc ctgaagagcg aaagcgatcg ctctggcgcc
     3421 tgaaacgctg aaccctaact tgttgaatac tgaggggact gcgggcgcca cacccgcatc
     3481 ctctccagga aaacagcctt cgaggctttc ttccgttgcg gttggggcta gatagggtgt
     3541 cctaacccca agagaacctg acgaatatag cactggaccc cggcctggcg caacgggcgc
     3601 cggctcgctc cagaccctcg agacgacggg acttcggacg ggttaccgtc gtctgggcag
     3661 ccgccgccgc ccccaagggg gcctacctcc gcgcggccaa atactccacg agggctgtca
     3721 tttcgtcctc ggtgagcgtc tcaccaaacg ccggcatgtt cgccaacggg tcgatccccg
     3781 ccgggtcggt gatccacctc cgcagccacg tgcccgaaga gcatcgagac gatgcctgtg
     3841 acgaccatca gagcgacggg tgcccgatgg tggagcggaa gagccggctc tgacgactcc
     3901 tgccgctgag ttgacgaatc ctgcaactgt cgcacattac attgcgggcg attgagcgga
     3961 aacggcgcga acacttttcc ggactgcggc tacctgccga cgagccgccg aaccccgcgg
     4021 acggcaaccg ccagaacctg gaacaccagc gccccgacga acaccaccat gaagaaagcc
     4081 atcacc
//
LOCUS       DBNGSH010000893         9067 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2997331, whole genome shotgun sequence.
ACCESSION   DBNGSH010000893 DBNGSH010000000
VERSION     DBNGSH010000893.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9067)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9067
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2997331"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="AC16CY_20515"
     CDS             <1..1065
                     /locus_tag="AC16CY_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3356247.1"
                     /translation="RIDGILYNIMQPPMKFRDAITSRIKIMSKLDIAEKRLPQDGRIK
                     IRFQDNGEMRDIDFRVSCLPTLFGEKIVLRLLDKTKLMLDMTKLGFESESLSKFEVAI
                     SKPWGMVLVTGPTGSGKTNTLYSSIARINTPETNIMTAEDPVEFNLMGVNQVQVRENI
                     GLNFAAALRSFLRQDPNIILVGEIRDFETAEIAVKAALTGHLVLSTLHTNDAPSTINR
                     LMNMGIEPFLVASSLNLICAQRLVRRICKNCTEPDPVPAPALMQAGFSAEDAKKVVPN
                     RGKGCEKCNNTGYKGRVGLYEVMEITEEIRELVLVGASALELRRKAVDEGMLTLRSSG
                     LRKIKEGVTTIEEVVRETVK"
     gene            1066..2187
                     /locus_tag="AC16CY_20520"
     CDS             1066..2187
                     /locus_tag="AC16CY_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3356248.1"
                     /translation="MQTLPELLQMVVQMQGSDLHITTSTPPQVRVHGHLTRLEGAALT
                     PSDTKQLTYSVLTDAQKKRFEETLELDFSFGIRGLARFRVNMFNQRGAVGAVYRLIPE
                     QIRSFQELNLPPVLATLAERPRGLVLVTGPTGSGKSTTLAAMIDKINKERRDHILTIE
                     DPIEFVHQHQGCLVNQREVHSDTESFSNALRAALREDPDIVLIGEMRDLETVEAALRI
                     AETGHLTLATLHTNSTSQTINRIIDIFPAHQQSQIRTQLSLVLEGIVCQALLPKVGGG
                     RVVALEIMVPTAGIRNLVREDKVHQIYSAMQTGQEKMGMQTMNQSLATLYQKRLITLE
                     TALMASSNRDELQDLINRGVGVVAGAGLGRTGTAGRPAR"
     gene            2234..3445
                     /locus_tag="AC16CY_20525"
     CDS             2234..3445
                     /locus_tag="AC16CY_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3356249.1"
                     /translation="MATFAYSGRTRAGQTVTGERVADSMDGAVAALRREQILVTQINP
                     VKEREKAQPKPKGRKVSPKNLAVFTRQFSVMIDAGLPLVQCLEILGTQEEDKNFAGVI
                     LATRTDVESGASLADAMKKHPKTFDSLFTNMIAAGEAGGILDAILKRLATYIEKNVKL
                     KSQVKSAMIYPIAVIVIAALVVGAILWKVIPTFANLFAGLGAQLPLPTRIVIAASNGL
                     VRFMPFVLVGIGASIFAFRAYYGTPGGRRVVDAVILKMPVLGLLMRKIAVARFCRTLS
                     TLLASGVSILEALDITARTAGNAIVEEAILKTRKSIEGGETIAAPLKETRVFPPMVVQ
                     MISVGEATGALDTMLGKIAEFYEEEVDVAVAGLLTLLEPIMIFFLGIVVGGIVIAMYL
                     PIFDLISKLTG"
     gene            3536..5224
                     /locus_tag="AC16CY_20530"
     CDS             3536..5224
                     /locus_tag="AC16CY_20530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3356250.1"
                     /translation="MNLRSRLSTIIAARVVLVTLLMGTAILIQINRPGSFPVDPFFAL
                     IGLTYALSVFYLAMLRFVERHLWLIDLQFGVDALLVSAFIHVTGGIASNFSSLYLLPI
                     IAASMIRYRRVAIHVASLSAALYLALVTVQYVDVLPLARRFSPAVDLPTLRVAQYTVA
                     INLGGFLAVAMLAGSLADSLRSAGAKLEHASLAIRDLRAFNEYVINSLLSGLITTDGD
                     GRILTLNRAAVSILGIPAARAAGRDVCEVMQTPDWVRPRLPGLSEGRSLRVDCPHRPP
                     DGRVLEMGLTFSSLTFPDGRKGYLLSFQDVTDVRRLEHEARLRQRLAAVGEMAAGIAH
                     EIRNPLASMSGSIQVLRQELPLSEDQAQLMDIVLRESERLNDTIRSFLAYARPQPSAP
                     ARLDVRRILQDTALLLRNSAEVRGDHVIDVEAPREPVWCEADESQIRQVVWNLATNGL
                     RAMASGGRLLLAVVADRTAGVDESVVLTVEDQGCGIAPEDLDSIFQPFRSSFERGTGL
                     GLAIVHRVVTDYNGTIQVSSKVGVGTKVSVRLPAHAGTGGSVPAGDATRRSAVA"
     gene            5221..6693
                     /locus_tag="AC16CY_20535"
     CDS             5221..6693
                     /locus_tag="AC16CY_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3356251.1"
                     /translation="MIRPSPATPPPDAVEAPDPGARPRILVVDDEPSMRDMLRIVLRR
                     DGYDVSVAANGAEAIDFIGRERLDLLLSDIRMPDISGVDVLRAAKEANRDIIAFMMTA
                     FASTDTAVEAMRLGAVDYFTKPFSMDELRLKVRQHLEALRLKRENVLLKRALNTSHQF
                     SNIIGRSDVMLELFKMIETIARTTSTVLITGESGTGKDLVARAVHVNSLRREHPFVAL
                     NCGGMPETLLESELFGHVRGAFTGADTNKKGLIEVAEHGTIFLDEIGEMNQAMQVKLL
                     RVLQDRRFRRLGGTDEVQADIRVIAATNQDLQKMVGEGRFREDLYYRINVIHVHLPPL
                     RDRREDVPLLADHFLAKYTKGLGKPVQSVSHEAQELLAAYAWPGNVRELENVIERAVA
                     LEQSATVLPESLPPHVRAGATMLPRTASASAAPGQPASSPGLPDLTEGFDLEAVGEEF
                     YRHYIALALERAGGVQTRAAEMLGMSFRSFRYYAKKLNLR"
     gene            7050..7580
                     /locus_tag="AC16CY_20540"
     CDS             7050..7580
                     /locus_tag="AC16CY_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3356252.1"
                     /translation="MKRLQKNEGFTLIELLIVVAIIGIIAAIAVPGLLRARMSGNETS
                     AIGSVRTVNTSEVGYSSAAGNGGYAVLLATLAAPCPGSQQAFISPDLRLDPSVKSGYT
                     VALAAGAAAVVGPNDCNGTATQTAYYATAIPLTLGTTGNRSFASTAGGSIFYNTTAVA
                     PTEAQMAPGGGGTTIQ"
     gene            7640..8212
                     /locus_tag="AC16CY_20545"
     CDS             7640..8212
                     /locus_tag="AC16CY_20545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3356253.1"
                     /translation="MVQTDDRPALPRVDGASRGQDPRGFTLVELLVVVGIIGIVAGIA
                     APALLRARMNSNETSAIGSVRIINTAQLDYSAACASGFFALTFPTLAVGPGGSRGFLS
                     QDLSYALVPQKSGYIYTMAAGATGIAGPPDCNGAATTTAYYITALPASVGTSGTRGFA
                     GNNSGAIWQDVSGAAPPEPFVFGPGVSPVQ"
     gene            8301..>9067
                     /locus_tag="AC16CY_20550"
     CDS             8301..>9067
                     /locus_tag="AC16CY_20550"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR3356254.1"
                     /translation="MNKAGRYRVPLILFALLGLVASIYASYVHYKLLTVPDYTSLCEV
                     SETVSCQQVFQSQYGSVLGVPVAAGGAIWAGLVLLLAAWGMRKPESPDAARVAGYVFV
                     LGTLGLAAAFYFGYVSFFVLGSACVVCITMYVSIAGVFLLSAAAAGPLGALPSLISKD
                     LSSVWRTPVTGVLTALWLLASVGLVMAFPREQPVSAASSEQAAPAVPQETLTPEQIAE
                     WEQWLAAQTPSTVADVLPSGDTKILVLKFNDFQCPSCR"
BASE COUNT         1611 a         2953 c         2960 g         1543 t
ORIGIN      
        1 cgcatcgacg gcatcctcta caacatcatg cagccgccga tgaagttcag ggacgcgatc
       61 acctcgcgca tcaagatcat gtccaagctg gacatcgccg agaaacggct cccgcaggac
      121 ggccgcatca agatccggtt ccaggacaac ggcgagatgc gcgacatcga cttccgcgtc
      181 tcgtgcctgc cgacgctgtt cggcgagaag atcgtccttc gtctgctgga caagacgaag
      241 ctcatgctcg acatgaccaa gctcggattc gagtccgagt cgctcagcaa gttcgaggtc
      301 gccatctcca agccgtgggg catggtgctc gtcaccggcc cgaccggatc gggcaagacg
      361 aacacgctct attcgtcgat cgcgcgcatc aacacgcccg agaccaacat catgacggcc
      421 gaggatccgg tcgagttcaa cctgatgggc gtcaaccagg tccaggtgcg cgagaacatc
      481 gggctgaatt tcgccgcggc gctccgcagc ttcctccgcc aggacccgaa catcatcctg
      541 gtgggcgaga tccgcgactt cgagacggcg gagattgcgg tcaaagcggc attgacgggc
      601 cacctggttc tctcgacgct ccacaccaac gacgcgccga gcacgattaa ccgtctcatg
      661 aacatgggca tcgagccctt cctggtcgcc agctcgctca acctgatttg cgcccagcgc
      721 ctcgtgcgca gaatctgcaa gaactgcacg gaaccggacc ccgtgccggc gcccgcgctg
      781 atgcaggccg gcttctctgc cgaagatgca aagaaggtgg tcccgaaccg ggggaagggc
      841 tgcgagaagt gcaacaacac cggatacaag ggacgtgtcg gcctctacga ggtcatggaa
      901 atcaccgaag agatccgtga gctcgttctg gtcggcgcct cggcgctcga actgcgacgc
      961 aaggccgtgg acgaggggat gctgacgctt cgaagcagcg ggctcaggaa gatcaaggaa
     1021 ggcgtcacga ccatcgaaga ggtcgtcagg gaaacggtga aatagatgca gacactgccc
     1081 gaactgctgc agatggtggt gcagatgcag gggtcagacc ttcacatcac gaccagtacc
     1141 ccgccacaag tgcgggtcca cggccatctc acgagactcg aaggggcggc gctgacgccc
     1201 tcggacacca agcagctgac ctacagtgtg ctcaccgacg cccagaagaa gcgcttcgag
     1261 gaaacgctcg aactggattt ctccttcggc atcagggggc tcgcccgctt ccgcgtcaac
     1321 atgttcaacc agcgcggcgc cgtcggggcc gtctaccgtc tgattccgga gcagatccgc
     1381 agctttcagg agttgaacct gcccccggtg ctcgccacgc tcgccgagcg tccgcgtgga
     1441 ctcgtgctgg tcaccggtcc gacgggctcc ggcaagtcga cgacgctcgc cgcgatgatc
     1501 gacaagatca acaaggaacg ccgggaccac atcctgacga tcgaggatcc gatcgaattc
     1561 gtgcaccagc accagggctg tctcgtgaat cagcgagagg tccacagcga taccgagagc
     1621 ttttcgaacg cgctgcgtgc cgccctccgc gaagatcccg acatcgtgct gatcggtgaa
     1681 atgcgggacc tcgagacggt cgaggcggcg ctccgcatcg cggagaccgg ccacctgacg
     1741 ctggccacgc tgcacaccaa ctcgacgtcg cagacgatca accgcatcat cgacatcttc
     1801 cctgcgcacc agcagagcca gatccgcacg cagctgtcgc tggtgctcga gggcatcgtc
     1861 tgtcaggcac tgctgccgaa ggtgggaggc ggacgcgtgg tggcgctcga gatcatggtg
     1921 ccgacggccg gcatcaggaa cctggttcgc gaggacaagg tgcaccagat ctactcggcg
     1981 atgcagacgg gccaggagaa gatgggcatg cagacgatga accagtcgct ggcaacgctg
     2041 tatcagaagc ggctgatcac gctcgagacg gcgctgatgg cgtcgtcgaa ccgcgacgag
     2101 ctgcaggacc tgatcaatcg aggcgtcggc gtcgtggcag gcgccggact cgggcgcacc
     2161 ggcacggcgg ggcgtcctgc cagatgaggc gtgaatgaca cggcgaaacc ggaactgggc
     2221 ggagtaagtc gtcatggcta catttgccta tagcggacgg acccgcgcgg gacagacagt
     2281 gacaggagag cgcgtcgccg actcgatgga cggcgccgtc gccgcgctgc ggcgggagca
     2341 gattctcgtc acgcagatca acccggtcaa ggagagggag aaggcccagc ccaagccgaa
     2401 gggccggaag gtctcgccga agaacctggc ggtcttcacg cggcagttct ccgtgatgat
     2461 cgacgcggga ctcccgctcg tgcagtgcct cgagattctc ggaacgcagg aagaggacaa
     2521 gaacttcgcc ggcgtcatcc tggccacgcg caccgacgtc gaaagcggtg catcgctggc
     2581 cgacgccatg aagaagcacc ccaaaacgtt cgattcgctc ttcaccaaca tgattgccgc
     2641 cggtgaagcg ggcggtatcc tcgatgcgat tctgaagcgg ctcgccacct acatcgagaa
     2701 gaacgtcaag ctgaagagcc aggtgaagtc cgcgatgatc tacccgatcg cggtgatcgt
     2761 gattgccgcg ctcgtcgtcg gcgcgatcct ctggaaggtg attccgacct tcgcgaacct
     2821 gttcgcgggg ctcggcgccc agctgccgct gcccacgcgg atcgtcatcg cggcgagcaa
     2881 cggcctggtc cgcttcatgc cgttcgtcct ggtcggcatc ggcgcgtcca tcttcgcgtt
     2941 ccgcgcctac tacggcacgc ccggcgggcg gagagtggtt gacgccgtca tcctgaagat
     3001 gccggtgctc gggttgctga tgcgcaagat tgccgtcgcc cgattctgcc ggacgctgtc
     3061 cacgctgctg gcgtcgggcg tctcgatcct cgaagccctc gacatcacgg ctcgcaccgc
     3121 cggcaacgcc atcgtggaag aggccatcct gaagacgcgg aagagcatcg agggcggcga
     3181 aacgatcgca gcgccgctca aggagacgcg tgtcttcccg cccatggtgg tgcagatgat
     3241 cagcgtcggc gaagcgacgg gcgcgctcga cacgatgctg ggcaagatcg ccgagttcta
     3301 cgaggaggaa gtcgacgtgg ccgtcgccgg cctgctgacg ctgctcgaac cgatcatgat
     3361 cttcttcctg ggcatcgtgg tcggcggcat cgtcatcgcg atgtatctgc cgatcttcga
     3421 cctgatcagc aaattgacgg gataatactc gtcaacgccc agcttccaac tcccaactcc
     3481 caactccgcg ggatgccttg ggagttgggt cttgggagtt gggagttgag tgagtatgaa
     3541 tctgcgctcc cgtctctcga cgatcatcgc cgcgcgggtc gtgctggtca ccctgctgat
     3601 ggggacggcc attctcattc agatcaaccg gccgggatcc tttccggtcg atccgttctt
     3661 cgcgctcatc gggctgacct acgcgctgag cgtgttctat ctcgccatgc tgaggttcgt
     3721 cgagcgccat ctctggctga tcgacctgca gttcggcgtc gacgccctgc tggtgtccgc
     3781 tttcatccac gtgacggggg gcatcgcgag caatttctcg tcgctctacc tgctgccgat
     3841 catcgctgcg agcatgatcc ggtaccggcg cgtggcgatc cacgtcgcct cgctcagcgc
     3901 tgcgctgtac ctggcgctcg tgacggtcca gtacgtggac gttctgccgc tggcccgccg
     3961 cttctcgccg gcggtggatc tgcccacgct gcgcgtcgcc cagtacacgg tggccatcaa
     4021 cctcggcggg ttcctcgccg tcgcgatgct ggccgggtcg ctggccgaca gcctgcgttc
     4081 ggctggcgcg aaactggaac acgcctcgct cgcgatacgg gacctgcgcg ccttcaacga
     4141 gtacgtcatc aacagtctcc tgagcggcct catcacgaca gacggcgacg gccgaatcct
     4201 gacgctcaac cgggcggccg tgtccatcct cggcatcccc gccgcacggg ccgccgggcg
     4261 cgacgtctgc gaggtgatgc agacgccgga ctgggtacgg ccgcggctgc ccgggctgtc
     4321 cgaggggcgc agcctcagag tcgattgtcc acaccggccc ccagacggcc gcgtgctcga
     4381 gatgggattg accttcagct ctctgacgtt cccggatggc cggaagggct acctgctgtc
     4441 gttccaggac gtcaccgacg tgaggcggct cgagcacgaa gcgcgccttc gccagcggct
     4501 cgccgcggtc ggcgagatgg cggccggcat cgcccacgag attcggaatc ctctggcctc
     4561 gatgtcgggc tccatccagg tgctgcgcca ggaactgccg ctgagcgagg atcaggccca
     4621 gctgatggac atcgtgctgc gcgaatccga gcgcctgaac gacaccatcc ggtcgtttct
     4681 ggcgtacgct cggccgcagc cgtcggcgcc tgccaggctg gacgtgcgac gcattcttca
     4741 agacacggcg ctgctccttc gcaacagcgc cgaagtgcgc ggcgaccatg tgatcgacgt
     4801 cgaggcgccc agggagcccg tctggtgtga agccgacgag agccagatcc gtcaggtcgt
     4861 ctggaacctc gcgaccaacg gtcttcgcgc gatggccagc ggcggccggc tgctgttggc
     4921 ggtcgtcgcc gaccggacgg ccggtgtgga cgagtcagtg gtcctcaccg tcgaggatca
     4981 gggctgcggc atcgcgcccg aggatctcga cagcatcttc cagccgttcc gaagctcctt
     5041 cgagcgagga acgggtctcg ggctggcgat cgtgcatcgg gtggtcaccg actacaacgg
     5101 cacgattcag gtgtcgtcga aggtgggcgt cgggacgaag gtcagcgtcc ggctccccgc
     5161 tcacgcggga accggcggca gcgtgcccgc tggcgacgcg acgcgccgga gcgcggtcgc
     5221 atgatccgcc cctcgccggc cacgcccccg cccgacgcgg tggaagcccc cgatcccggc
     5281 gccaggcccc gcatcctcgt cgtcgacgac gagccgtcca tgcgggacat gctgcgcatc
     5341 gtgctgcgcc gagacgggta cgacgtgtcg gtggccgcca acggagccga ggcgatcgac
     5401 ttcatcggac gcgagcgcct cgacctgctg ctctcggaca tccgcatgcc cgacatcagc
     5461 ggcgtggacg tgctccgcgc ggcgaaagag gccaaccggg acatcatcgc gttcatgatg
     5521 acggcgttcg cctcgaccga caccgccgtc gaagccatgc ggctcggcgc cgtggattac
     5581 ttcaccaagc cgttcagcat ggacgaactg cggctcaagg tgcggcagca cctcgaggcg
     5641 ctccgcctca agcgcgagaa cgtcctgctg aagcgggccc tcaacacgag ccaccagttc
     5701 tcgaacatca tcggccgcag cgacgtgatg ctcgagctct tcaagatgat cgagaccatc
     5761 gccaggacca cgagcacggt gctgatcacc ggcgagtcgg gcaccggcaa ggatctggtg
     5821 gcgcgggcgg tgcacgtcaa ctcgctgcgg cgcgaacatc cgtttgtcgc cctcaactgc
     5881 ggtggcatgc cggagacgct gctcgagtcg gaactcttcg gccacgtacg aggcgcgttc
     5941 accggcgccg atacgaacaa gaagggcctg atcgaagtcg ccgagcacgg caccatcttc
     6001 ctcgacgaga tcggggagat gaaccaggcc atgcaggtca agctgcttcg cgtgctccag
     6061 gatcggcgat tccggcgtct gggcggcacc gacgaagtgc aggccgacat ccgggtgatc
     6121 gccgccacga accaggacct gcagaagatg gtgggcgagg gacgcttccg ggaggatctc
     6181 tactaccgga tcaacgtgat ccacgtgcac ctgcctccgc tgcgcgaccg gcgtgaggac
     6241 gtgccgctgc tggcggacca ctttctcgca aagtacacga aggggttggg caagccggtg
     6301 cagagcgtgt cgcatgaggc gcaggaactg cttgcggcgt acgcatggcc gggtaacgtg
     6361 cgggagctgg agaacgtgat cgagcgcgcg gtggccctcg agcagagcgc cacggtcctg
     6421 ccggagagcc tgccccccca cgtcagggcg ggtgccacga tgcttcccag gactgcatcg
     6481 gcaagcgccg cccctggcca accggcgtcg tcgcccggcc tgccggatct gacggaaggc
     6541 ttcgacctgg aagcggtggg tgaggagttc taccggcact acattgccct ggctctcgag
     6601 cgcgcggggg gcgtgcagac cagggccgct gaaatgctcg gaatgagctt ccgctccttt
     6661 cgctactacg ccaagaagct caacctgcgt tgaaccgggg cggcagactg acgaggaccg
     6721 ccaattcttg acaccctggg ttgacacttc ttgtcaaccg gcctggtaaa tggcacctga
     6781 cgaacgggtg gggccggacc gtccaaagac ggtaagccta cagtggcaaa tcagttacgc
     6841 cgatggagtg ggtaagggtt gagcgtggca ccacgcttgc ctctcggggg ttgtccacct
     6901 tcgggattgc tcagggtgcc ctgggcgacc cgggtgacgg tgagtaaggt tgtcctgggc
     6961 agtgtcgaag gggaacgagg cgccgtgtcg gaacggcaat cggttaaacg tcgggccttc
     7021 cggcccgagc gcactcaagg agtagggttg tgaagagact acagaagaac gaagggttca
     7081 cgcttatcga gctgctgatc gtcgtggcga tcatcggcat catcgcggcg atcgcggtgc
     7141 ctggtctgct tcgggcccgc atgtcgggta acgaaacgtc ggcgatcggg tctgtgcgga
     7201 ccgtgaacac gtcggaagtg ggctattcgt cggcggcggg caacggcggg tacgcggtgc
     7261 ttctcgcgac gctggcggca ccgtgcccag gcagtcagca ggcgttcatc tcgcctgacc
     7321 tgcgcctcga cccgtcggtc aagagcggct acacggttgc gctggccgcc ggggccgcgg
     7381 cggttgtcgg gccgaacgac tgcaacggca cggcgacgca gacggcgtac tatgcgacgg
     7441 caattccgct gacgctcgga acgaccggca accgtagctt cgcgtctacg gctggcggca
     7501 gcatcttcta caacacgacc gcggttgcgc cgacggaggc ccagatggcg cccggcggcg
     7561 gcggcacgac gattcagtaa ccccgcgatt accaccgggg atgcccaccg tgggcatccc
     7621 cacctctcta atcactccca tggtacaaac cgacgacaga ccggcgctgc cacgagtgga
     7681 cggcgcgagc cgcggccagg accctcgcgg gttcacgctc gtcgagctgc tggtggttgt
     7741 cggcatcatc ggcatcgtcg ccgggattgc tgctccggcc ctgctccgtg cccggatgaa
     7801 cagcaacgag acgtcggcga ttggatctgt tcgcatcatc aacacggctc agctcgacta
     7861 ctcggcggca tgtgcgtccg gtttcttcgc tctcacgttc ccgacgctgg ccgtgggccc
     7921 cggcggttcg cgcggattcc tttcccagga tctgtcatac gcgctggtgc cgcagaagag
     7981 cggctatatc tacacgatgg cggccggagc caccggtatc gccgggccgc cggactgcaa
     8041 cggggcggcc acgaccactg cctattacat aaccgcgctt cccgcaagcg tcggcacgtc
     8101 aggcaccaga gggtttgcag gcaacaattc gggcgccatc tggcaggatg tgagcggcgc
     8161 cgcgcctccc gagcccttcg tcttcggacc aggcgtctcg cccgttcaat agccgggtct
     8221 tcacgtcttc ttacccgtcg gccgacggcc ccgacgggca tttcgaactg acgggagcct
     8281 ccgctataat cgccccgatc atgaacaagg ccggccgtta tcgcgtcccg ctgatcctct
     8341 tcgctctgct cggtctcgtt gcgtcgatct acgcctccta cgtccactac aagcttctga
     8401 cggttcccga ctacacgagc ctctgcgagg tcagcgagac ggtgagctgt cagcaggtgt
     8461 tccagagcca gtacggaagc gttctcggag tgccggtcgc ggccggcggc gccatctggg
     8521 ccgggctggt gctgctgctc gcggcctggg gcatgaggaa gcccgagtct ccggacgccg
     8581 cccgggtcgc aggctacgtt ttcgtactcg gcacgctcgg tctcgcggca gcgttctatt
     8641 tcggctacgt gtccttcttc gtactgggct cggcatgcgt ggtctgcatc acgatgtacg
     8701 tgagcattgc tggcgtgttc ctgctctcgg cggcggccgc cggaccgctc ggagcactgc
     8761 cgtcgctgat aagcaaagac ctcagtagtg tgtggcgtac accggtcacg ggggtactga
     8821 cggcgctgtg gcttcttgcc tcggtcggac tcgtcatggc cttcccgcgg gaacagccgg
     8881 tgagcgccgc ctcgtccgag caggcggctc cggcagttcc gcaggaaacg ctgacgcccg
     8941 agcagatcgc cgagtgggaa cagtggctcg cggcacaaac gccctcgacg gtggccgacg
     9001 tcctgccgtc gggcgacacg aagatcctgg tgctcaagtt caacgacttc cagtgtccgt
     9061 cctgccg
//
LOCUS       DBNGSH010000894         3598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4697494, whole genome shotgun sequence.
ACCESSION   DBNGSH010000894 DBNGSH010000000
VERSION     DBNGSH010000894.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4697494"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC16CY_20555"
     CDS             <1..186
                     /locus_tag="AC16CY_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356255.1"
                     /translation="LFMPLFREIDEMLYQWEEPFVIDDRQFRERFHVVPTDVDRAAAD
                     TLEWATRHYGRGTSRTT"
     gene            454..762
                     /locus_tag="AC16CY_20560"
     CDS             454..762
                     /locus_tag="AC16CY_20560"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3356256.1"
                     /translation="MSNKLYVGNLPYETTESDLQALFEGAGQVSTINIVRDRTTGQAR
                     GFAFVEMGDAEGAQRAISELDKHQYGGRSLTVNEAKPMTPRGNGGGDFGGRQRRDARW
                     "
     gene            complement(861..2795)
                     /locus_tag="AC16CY_20565"
     CDS             complement(861..2795)
                     /locus_tag="AC16CY_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIR3356257.1"
                     /translation="MSDSPAVAERSAAHESEAQGATDRLLPVTLTALGVVYGDIGTSV
                     LYALRECFFGPHSVPPTHENVLGVLSLIIYSLLLVISIKYVAIVMRADNQGEGGVLAL
                     TSLIPGRAGTPGVARLATGRPVLIALGIFGTALLYGDGMITPAVSVLSATEGLEVATP
                     LFQPYVVPATVAILVGLFLIQKYGTHRVGGLFGPIMAIWFLTIAGLGIVWTARYPAVL
                     AAFDPRHAVAFFTDNGFIGFVVLGAVFLVVTGGEALYADMGHFGRRPIRLAWFALVLP
                     ALILNYLGQGALLLLEPAAEHPFFMLAPSWALLPLIGLSTAAAIIASQALISGSFSIT
                     RQAIQLGLAPRLEVEHTSASEMGQIYVPQVNWALMFATVLIVVGFGSSSALASAYGIA
                     VTLTMIITVLLLYIVMTELWRWPKPAAAAVTTMFLIIDGAFFGANALKIMQGGWVTLA
                     VALLLFTLMTTWKTGRRLVAERLTARALPLPDFIATVEAMKPIRVPGTAVFMTAQPTG
                     TPPALAHNLQYNKMLHQHVVVLMVTTAQQPHVPPEERVFVEPLGHDLFYVRVQYGFME
                     DPDVPQALEQARQQGLVLDSGDLTYFLGRETIIVSRRKGMAIWRERLFMLMARNAMRA
                     TAFFRLPTERVVELGVQVEM"
     regulatory      complement(2794..2966)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            3100..>3598
                     /locus_tag="AC16CY_20570"
     CDS             3100..>3598
                     /locus_tag="AC16CY_20570"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3356258.1"
                     /translation="MTKTEWFLAELESEAAITRRVLAEVPEGKRDWKPHDRSMAFGYL
                     TDLTANILSWVGLAITLDELDLAPKSGPTYRPMPAQTSAEAIAALDAAVAQARVALQK
                     TNDAHLATSWRLLAGGTVVMEQRRHDVIRDTFLHSSHHRGQMTVYLRLLGARVPSVYG
                     PSADDR"
BASE COUNT          679 a         1174 c         1182 g          563 t
ORIGIN      
        1 ctgttcatgc cgctgttccg ggagatcgac gagatgctct atcaatggga ggagccgttc
       61 gtcatcgacg atcgacagtt ccgcgagcgc ttccatgtcg tcccgacaga cgtcgatcga
      121 gccgccgccg ataccctcga gtgggcgacc cgtcattacg gacgcggcac gtccaggacc
      181 acctgaagag atcgacgcgc ggtacggatc gacccggtgg cggcgctgca gagcgagtga
      241 ctcgctgacg acgacgccgc ccgcactgag acggcagaat ccgttgcgcc gtcgtgtact
      301 gcctgatata ctcgcatcgg cttctgacag ctgcgcttcc ggccttccgc atcagatccg
      361 agcgttcctc gcctctcctg cgtttcgtca tcgagcattt cgctcgagcc tgccatcaga
      421 ttcagacgca tcctcgagat gcaggagtag tgaatgagca acaaactgta cgtcggcaac
      481 ctgccgtatg aaacgaccga gtccgacctt caagcactgt tcgaaggcgc gggccaggtg
      541 agcaccatca acatcgtccg tgaccgcacc accggtcagg cgcgcggatt cgcgttcgtc
      601 gaaatgggcg acgccgaagg cgcacaacgc gcgatcagcg aactggacaa acaccagtac
      661 ggcggccgca gcctgacggt gaacgaagcg aagccgatga cgccccgcgg caacggcggc
      721 ggtgacttcg gcgggcgcca gcgccgcgac gcgcgctggt aagcgtcacg cccgcgcggg
      781 cgtgacgtct cgatcagccc ccgggtccgc acgagcggat ccggggcgct tcacgcgtgg
      841 atcaccggtc catccggaac tcacatctcc acttgaacgc cgagctccac cacgcgttcc
      901 gtcggaagac ggaagaacgc ggtggcccgc atggcgttgc gggccatcag catgaacagc
      961 cgttcgcgcc agatcgccat ccccttgcgg cgcgacacga tgatggtttc gcggccgagg
     1021 aagtaggtca ggtcgccgga atcgagaacg agcccctgct gacgcgcctg ctccagcgcc
     1081 tgcggcacat cgggatcctc catgaagccg tactggaccc gcacgtagaa gagatcgtgc
     1141 ccgagaggct cgacgaagac gcgctcctcc ggcggcacgt gcggctgctg cgcggtcgtg
     1201 accatcagca ccaccacgtg ctggtgcagc atcttgttgt actgaagatt gtgcgcgagc
     1261 gccggagggg tgcccgtcgg ctgcgccgtc atgaacacgg ccgttcccgg gactcggatc
     1321 ggcttcatcg cttcgaccgt cgcgatgaaa tccggcagcg gcagtgcgcg agccgtcagc
     1381 cgctccgcca ccagccggcg cccggtcttc cacgtcgtca tcagcgtgaa cagaagcagc
     1441 gcaacggcga gcgtcaccca gccgccctgc atgatcttca gggcgttggc cccgaagaac
     1501 gcgccgtcga tgatcaggaa catcgtcgtg accgccgccg ccgcgggctt cggccatcgc
     1561 cacagctccg tcatgacgat gtacagcagc agcacggtga tgatcatggt cagcgtcacg
     1621 gcgatcccgt aggccgaggc cagggcactc gacgacccga acccgacgac gatcagcacg
     1681 gtcgcgaaca tcagcgccca gttcacctgc ggaacgtaga tctgtcccat ttcgctcgcc
     1741 gacgtgtgct cgacctcgag ccgtggagcc agcccgagct gaatggcctg acgggtgatc
     1801 gaaaacgatc cggagatcag cgcctgcgac gcgatgatcg ccgccgcggt cgagaggccg
     1861 atcagcggca acagcgccca ggacggcgcg agcatgaaga aggggtgctc ggccgcgggc
     1921 tcgagcagca gcaaggctcc ctgacccagg tagttcagga tcagcgccgg cagcacgagg
     1981 gcaaaccagg ccagccggat cgggcgcctg ccgaagtgtc ccatgtcggc atacagcgcc
     2041 tcgcctccgg tcacgaccag gaacacggcg ccgagcacga cgaagccaat gaagccgttg
     2101 tccgtgaaga acgccaccgc atgccgggga tcgaacgccg ccagcacggc cggataacgg
     2161 gcggtccaga cgatgcccag gccggcgatc gtcaggaacc agatcgccat gattggaccg
     2221 aacagccctc cgacgcggtg agtcccgtac ttctggatga ggaacagccc gaccaggatc
     2281 gcgacggtgg cggggacgac atacggctga aacaacggcg tggcgacctc gaggccttca
     2341 gtcgcgctca gcaccgaaac cgccggcgtg atcatgccgt cgccgtagag cagcgcggtt
     2401 ccgaagatgc ccagggcgat gagaacaggc cggccggtcg cgagccgggc cactcctggt
     2461 gttcccgcgc ggcccggtat caacgatgtc agcgccagta cacctccctc accctggttg
     2521 tcggcgcgca tgacgatcgc gacgtacttg atcgagatca ccaggagcag cgagtagatg
     2581 atcagcgaca gcacgccgag cacgttctcg tgcgtcggcg ggaccgagtg cggcccgaag
     2641 aagcactccc gcagggcgta cagcacgctc gtcccgatgt cgccatagac gacgccaaga
     2701 gcggtgagcg tgacgggaag caggcgatcg gtggcgccct gcgcctcgga ctcgtgcgca
     2761 gcggaccgct cggcgacggc gggtgaatcg gacacagacc ctccaagggc cgacggggtt
     2821 agctgacggg ctcgaggttg gagtcctcta ccgtcggcct tcgcctttca ggcgccggcg
     2881 cgacggatac gccccttgga agcgtgatta cttccggtgg gtcccccgtt tcgctcgcaa
     2941 gcgagcgaat ttggctcttg gttgattatc ggcggaatcg ggccggccgc gataggaccg
     3001 tagcggcgac cccctgagct tcactttttc acgatcgtct gccaccgtgt ggggaagaat
     3061 ggctggagga gtggaattat ttgacccggc tggttgacta tgacgaagac tgaatggttc
     3121 ctggccgagc tcgagagcga ggcggcaatc acccggcgcg tgctcgcgga ggtgccggaa
     3181 ggaaagcggg actggaagcc gcacgaccgc tccatggcgt tcgggtacct gacggatctc
     3241 accgcgaaca tcctgtcgtg ggtgggcctg gccatcacgc tcgacgagtt ggatctcgcg
     3301 ccgaagagcg gtccgacgta ccggccgatg ccggcccaga ccagtgccga ggcgatcgcc
     3361 gcgctggacg cggctgtcgc tcaggcgcgt gtggcgctgc agaagacgaa tgatgcccac
     3421 ctcgcgacgt cgtggcggct gctggcggga ggtacggtcg tgatggaaca acgccgtcac
     3481 gatgtcattc gggacacctt ccttcattcg tcgcaccatc gcggacagat gacggtgtat
     3541 ttgcgtttgc tgggagcgcg tgtgccgtcg gtctacggtc cgtcagcgga cgaccgtc
//
LOCUS       DBNGSH010000895         4549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2659198, whole genome shotgun sequence.
ACCESSION   DBNGSH010000895 DBNGSH010000000
VERSION     DBNGSH010000895.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2659198"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="AC16CY_20575"
     CDS             complement(<1..295)
                     /locus_tag="AC16CY_20575"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR3356259.1"
                     /translation="MRRAFASSAVRIALAFCSLAAASAVPSSAQTPPPPKIWTVTAGA
                     GAALTSGNSDTSSLNAAYDITWDPPNRHVVKSDALALRARTEDELTANRVGVNV"
     gene            complement(299..1453)
                     /gene="asd"
                     /locus_tag="AC16CY_20580"
     CDS             complement(299..1453)
                     /gene="asd"
                     /locus_tag="AC16CY_20580"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR3356260.1"
                     /translation="MRVGIIGWRGMVGSVLVQRMREERDFDLVEPSFFSTSQAGAAAP
                     DIGRRVPAVKDARDERELTAMDVLISCQGGEYTTEIHPRLRRAGWNGYWIDSARALRM
                     SDQAVIILDPVNRPVIDRALAGGIKDYIGGNCTVSLMLIAMDGLFRADLVEWITSMTY
                     QAASGGGAKHMRELVSQMGRSYASVADLLADPSSSILDIDAALIATQRGPDFPIDNFE
                     HPLAGSLLPWIDADLGNGQSREEWKAQAEGNKILGRSSQPIPIDGVCVRVGAMRCHSQ
                     ALTVKLRRDVPLADIERLLAGANDWVTVVPNEKHATLRGLTPTAVTGTLQVPIGRLKK
                     LSMGGDFLQAFTVGDQLLWGAAEPLRRMMRIIVGAPLDSPADRLTTAARH"
     gene            complement(1490..2635)
                     /locus_tag="AC16CY_20585"
     CDS             complement(1490..2635)
                     /locus_tag="AC16CY_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR3356261.1"
                     /translation="MPSALTTLTDDERLFRDSVFEFADREVRPLVRRMDDEAKMPRDL
                     IDKLFALGVMGIEIPESDGGGGATFFHAVLAVEALSRVDPSVAVLIDVQNTLVVNAIL
                     RWGSPAQQREFLPRLAQKAVGAYALSEAGSGSDAFALATRAVPRGNDFVLTGRKLWIT
                     NAAEADLFIVFANANPDEGHRGITAFLIERGAAGFTIGKKEDKLGIRASSTCELILEE
                     CAVGRDRVLGEVGKGYKVAIETLNEGRIGIGAQMIGLAQGAFDHAVQYTQERKQFGAA
                     IASFQGVQFQLARAATEIEAARLLVYNAARLRDARQPFLQEAAMCKLFSSEVAERVTS
                     LAVNLFGGYGFVKDYPVEKLYRDAKIGQIYEGTTNLQLQTIARQLLS"
     gene            2724..>4549
                     /locus_tag="AC16CY_20590"
     CDS             2724..>4549
                     /locus_tag="AC16CY_20590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3356262.1"
                     /translation="MLSSRAVPAFLLCLALVGASLVEPGSAQQGSTTPQPGQAEAPAS
                     QAPPQTGEAQPQTPVFRTGINFVRVDVIVGDRKDQPVTDLSQKDFELFEDGKPREIEQ
                     FSLVRTDGNPRPGAPPPRAIRNAADEEIEASREDTRVFVIFLDDYHVRRSNSISIREP
                     LKRFVQNQLRPTDMVAVMYPLTPVTVMSFTRNHNTILTAVERFTGRKFDYTPTNEIEY
                     QYQRLSTEEIERIRNDVVLTALEGLSVRLGALREGRKSVIFVSEGFTALLPPQMRRQD
                     ASMPANPFEVERQAKGQDSPREYTAEWFSMADIYQKMRNVFNAANRNNTSIYSLDPRG
                     LAPFEYGFDDLPGGPPPSFATDSRALRMTQDTLRTLSEETDGRAIVNRNTLDQGLAQV
                     TRDSSYYYLLGYTSQSPTDGKFHEISVRVRRRDVNVRARKGFWAVTNEDVIRAANPTP
                     EIAKPVQTALASISTSVQAGKYVRTWVGSERGESGKTRVTLVWEPLALPPGDRREPAV
                     HVSLLAATSAGSLVYRGRAPERSSTTPSAATGPLTAGPRRLVFEAPPGKLELRLTVEG
                     ASGGTLDQEIRDFVVPDLTTPQVVLSTPRVYRARTLPELRLIV"
BASE COUNT          756 a         1554 c         1527 g          712 t
ORIGIN      
        1 gaacgttgac gccgacgcgg tttgcggtca gctcgtcctc ggttcgtgcc ctcagcgcaa
       61 gcgcgtcgga cttgaccacg tgcctgttcg gcgggtccca ggtgatgtcg taggccgcgt
      121 tgagcgagga ggtgtccgag ttgccgctgg tgagcgcggc gccggcgccg gcggtgaccg
      181 tccagatctt cggggggggc ggcgtctggg ccgacgaggg gacagcggac gcagcggcga
      241 gcgagcagaa cgcaagcgcg attcggacgg cggacgaagc gaaggcgcga cgcacgagtc
      301 agtgcctggc ggcggtcgtc agccggtctg ccggcgagtc gagcggcgcg ccgacgatga
      361 tgcgcatcat gcggcgcagg ggttccgccg cgccccacag cagctggtcg ccgacggtga
      421 acgcctgcag gaagtcgcct cccatcgaca gcttcttcag gcgtccgatg gggacctgca
      481 gcgtcccggt gaccgcggta ggcgtcaggc cgcgcagcgt cgcgtgcttc tcgttcggca
      541 cgaccgtgac ccagtcgttg gcgccggcga ggagccgttc gatgtcagcc agcggcacgt
      601 cgcggcgcag cttcaccgtc agcgcctggc tgtggcagcg catcgcgccg accctgacgc
      661 agacgccgtc gatcggtatc ggctgcgacg agcggccgag gatcttgttg ccttccgcct
      721 gcgccttcca ctcctctcgg ctctgcccgt tgcccaggtc ggcgtcgatc cacggcagca
      781 ggctccctgc gagcgggtgc tcgaaattgt cgatggggaa gtcgggcccg cgctgcgtgg
      841 cgatcagcgc ggcgtcgatg tcgaggatcg aggacgacgg atcggcgagc aggtcggcga
      901 ccgacgcgta ggaccggccc atctgcgaca ccagctcccg catgtgcttg gcgccgccgc
      961 ccgaggcggc ctgataggtc atcgacgtga tccactcgac gagatcggca cggaagaggc
     1021 cgtccatcgc gatcagcatg aggctgaccg tgcagttgcc gccgatgtaa tccttgatgc
     1081 ccccggcgag cgcgcgatcg atgaccgggc ggttcaccgg gtcgaggatg atcacggcct
     1141 ggtccgacat ccggagcgcc cgcgcggagt cgatccagta gccgttccac ccggcgcgcc
     1201 tcagccgcgg atgaatctcc gtggtgtact cgcccccctg gcaggaaatc aggacgtcca
     1261 tcgccgtcag ctcgcgctcg tcgcgcgcgt ccttgacggc gggcacgcgc ctgccgatgt
     1321 cgggcgccgc ggcgcccgcc tgggaggtgg agaagaagct gggttcgacc aggtcgaagt
     1381 cccgctcctc gcgcatgcgc tgcacgagga ccgagcccac catgccgcgc caaccgatga
     1441 tgccgactcg catgtaaata ttgtagccgg tagccggcgg cccgaagggc tacgacaaca
     1501 actgcctggc aatcgtctgc aattgcaggt tggtggtccc ctcgtagatc tgcccgatct
     1561 tcgcgtcccg atagagcttc tcgacggggt agtccttcac gaacccgtac ccgccgaaca
     1621 ggttgacggc gagcgacgtg acgcgttcgg ccacctcgga tgagaacagc ttgcacatcg
     1681 ccgcctcctg gaggaacggc tgacgggcgt cgcgcaggcg cgcggcgttg tagacgagca
     1741 gccgcgcggc ctcgatctcg gttgccgccc gcgcgagctg gaactgcacc ccctgaaacg
     1801 aggcgatggc cgcgccgaac tgctttcgtt cctgggtgta ctgcaccgcg tggtcgaacg
     1861 ccccttgggc gaggccgatc atctgcgcgc cgatgccgat gcgtccttcg ttgagcgttt
     1921 cgatggccac cttgtagccc ttgcccacct cgccgagcac gcgatcacgc ccgaccgcgc
     1981 actcctcgag aatcagctcg cacgtgctgc tggcccggat gccgagcttg tcctctttct
     2041 ttccaatcgt gaacccggcc gcgccgcgtt cgatcaggaa ggcggtgatg ccgcgatgcc
     2101 cctcgtcggg attcgcgttg gcgaagacga tgaagaggtc cgcctcggcg gcgttggtga
     2161 tccagagctt ccggcccgtc aggacgaaat cgttccctct cgggacggcg cgcgtggcca
     2221 gcgcgaacgc gtcgctgccc gacccagcct cggagagcgc gtacgcgccg accgccttct
     2281 gcgccaggcg cggcaggaac tcgcgctgct gcgccgggct gccccagcgg agaatcgcgt
     2341 tgacgaccag cgtgttctgc acgtcgatca gcacggcgac cgacggatcc acgcgcgaaa
     2401 gcgcttcgac cgccagcacg gcgtggaaga acgtcgcccc gccgccgcca tccgactcgg
     2461 ggatctcgat gcccatgacg ccgagcgcga agagtttgtc gatgagatcc cgaggcatct
     2521 tcgcctcgtc gtccatacgc cgcaccagag ggcggacctc gcgatccgcg aactcgaaca
     2581 cgctgtcgcg gaacagccgc tcgtcgtcgg tcagagtcgt aagggcactc ggcacggccc
     2641 ctatggtagc cggtagcccg tagccggcag cccatagacc gaagcgtccc acaacgccta
     2701 tcatcggaaa gagggcacga ctcatgctca gctcccgagc ggttccggcg tttctgctgt
     2761 gtctggcgct tgtcggcgcc agcctggtcg agcccggctc ggcgcagcag ggttcgacga
     2821 cgccgcagcc ggggcaggcc gaggcccccg cgtctcaggc tccgccgcaa acaggggagg
     2881 cgcagcccca gactccggtc ttccgcacgg gcatcaactt cgtgcgcgtg gacgtcatcg
     2941 tcggcgatcg caaggaccag cccgtcaccg atttgtcgca gaaagacttc gagctgttcg
     3001 aggacggcaa gccgcgggag atcgagcagt tctcgctcgt gcgcaccgac ggcaacccga
     3061 ggccgggcgc cccgccgccg cgcgcgatcc gcaatgccgc cgacgaagag atcgaggcca
     3121 gccgcgagga cacgcgcgtc ttcgtgatct ttctcgacga ctaccacgtc cggcgcagca
     3181 actcgatcag catccgcgag ccgttgaagc gcttcgtcca gaaccagctc agaccgaccg
     3241 acatggttgc cgtgatgtat ccgctgacgc cggtgaccgt gatgtccttc accaggaatc
     3301 acaacaccat cctgacggcc gtcgaacgct tcaccgggcg caagttcgac tacaccccta
     3361 cgaacgaaat cgagtatcag tatcagcgcc tctcgaccga ggagatcgaa cgcatccgca
     3421 acgacgtggt gttgacggcg ctcgaagggc tgtcggtcag gctgggggcg ctgcgcgagg
     3481 gccgcaagtc ggtcatcttc gtcagcgaag ggttcacggc gctgcttccg ccgcagatgc
     3541 gcaggcagga cgcgtcgatg ccggccaacc cgttcgaggt ggaaaggcaa gccaaggggc
     3601 aggactctcc cagggagtac acggcggaat ggttcagcat ggccgacatc taccagaaga
     3661 tgcggaacgt gttcaatgcc gccaatcgaa acaacacttc gatctattcg ctcgatccgc
     3721 gggggctcgc gccattcgag tacggcttcg acgacctgcc ggggggaccg ccgccgagtt
     3781 tcgccaccga ctcgcgcgcg ctgcgcatga cgcaggacac gctgcggacc ctgtccgaag
     3841 agaccgacgg gagggcgatc gtcaaccgga acacgctcga ccagggcctg gcccaggtca
     3901 ctcgcgactc gagctattac tatctgctcg gctacacgag ccagtcgccg accgacggca
     3961 agttccacga gatctcggtg cgcgtcaggc ggcgcgacgt caacgttcgc gcccggaagg
     4021 gattctgggc cgtcaccaac gaggacgtca tccgcgcggc aaatccaaca ccggagatcg
     4081 ccaagccggt gcaaaccgcg ctcgcctcga tctccacgtc agtgcaggcg ggcaagtacg
     4141 tgcgcacgtg ggtgggctcc gagcgcggcg agagtggaaa gactcgcgtg acgctcgtgt
     4201 gggagccgct ggcgctgcct ccaggcgatc gccgcgagcc ggcggtacac gtctcgctgc
     4261 tcgccgcgac gtccgcgggc agtctggtat accgggggcg cgctcccgaa cgatcgtcga
     4321 cgacaccctc cgccgcgacc ggtccgctga cggctgggcc gcggcgcctc gtgttcgaag
     4381 ctccaccggg taagctcgag ctgcggctca cggtcgaagg cgcctcgggc ggcacgctcg
     4441 accaggagat tcgcgacttc gtcgtgccgg acctgacgac gccgcaagtg gtgttgagca
     4501 cgccgcgcgt ctaccgcgcg cgcacgctgc ccgagctccg gctcatcgt
//
LOCUS       DBNGSH010000896         2232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2997852, whole genome shotgun sequence.
ACCESSION   DBNGSH010000896 DBNGSH010000000
VERSION     DBNGSH010000896.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2997852"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(372..1082)
                     /locus_tag="AC16CY_20595"
     CDS             complement(372..1082)
                     /locus_tag="AC16CY_20595"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3356263.1"
                     /translation="MTNTNPILAEQLAYYRARAREYDKWFRREGLHDLGEEHNARWRL
                     ELNRVYSALDAFAPRGDVLELAYGTGEWTIRLAEVATSVVGVDAAPEMRELALAKLQD
                     AGKSNVELWVDDLFSWQPDQDYDVVFFAFWLSHVPESHTDRFWRSVHDALRPGGRFFL
                     VDAARAVLDRNVIHGSRLPDRESATELRRLEDGREFRIFKRSFDPDRLARDLRDHGLD
                     ASFVETGEFFVYGLGRRA"
     gene            complement(1085..1903)
                     /locus_tag="AC16CY_20600"
     CDS             complement(1085..1903)
                     /locus_tag="AC16CY_20600"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR3356264.1"
                     /translation="MSVTALTSAEISLPAEADGQRVSDRIAVLADTVDVVGLTDNHAG
                     QPRMSPLAAVALAREQGVGTIVHVSCRDRNRLALQSQVVGAAALGAEGVLCMQGDPVA
                     GVDHVADMTATELIARAPEWSVPHSLAVGAVVNPFAEDLDRELRLLQRKVAAGATFVQ
                     SQMVFDLPRLEAFLARGEDMLAGVRVYASVALLRSERMAERACALPGVTIPEPACEQI
                     RRGGGIELARELAAGLAGVSRVDALHVFPLGAEAATREVAAEFRVTRGINSRRR"
BASE COUNT          420 a          766 c          711 g          335 t
ORIGIN      
        1 cgagatcctt ggcggccttc tcccagtcga cgaagaactc ctgggcggcg gggtccagga
       61 aggtgggtcg tcatcgaccg cgccctgcac gacgagctga cagcgggccc tacgaggcca
      121 acggagcgct tgctcaaggg gcagcgggca cccgcaacct gcccactgcc caagccgcgc
      181 tgctcgtcct gatccgtgac gaacgggatc cgacgacgcc gacgaggcgg ccatgcaacc
      241 cgctgcctct ggagaagtca catatcgcat agccaggacc gctaccgacg atctaccggg
      301 cgggacaact gtcgaggccg gtcgtatgtc ttgcttgcac acgggctccg cgtgagtagt
      361 cccaggtcgg gctaggcacg tcggccgagt ccgtagacga agaactcgcc cgtctcgaca
      421 aagctcgcgt caaggccgtg gtcacgaaga tctcgcgcga gccggtcggg atcgaacgaa
      481 cgcttgaaaa tccgaaactc ccgaccgtcc tcgagtcgcc tgagctcagt cgcgctctcg
      541 cgatcgggca gtcgcgaacc gtgaatgacg ttcctgtcca ggaccgcgcg tgcggcatcc
      601 acgaggaaga agcggcctcc ggggcgaagc gcgtcgtgga cgctgcgcca gaaccggtcg
      661 gtatggctct cgggaacgtg cgaaagccag aacgcaaaga agacgacgtc atagtcctga
      721 tctggctgcc agctgaagag atcgtcaacc cagagctcga cattgctctt accggcatcc
      781 tgcagcttcg ccagagcgag ttcgcgcatc tccggcgctg catcgactcc gactacactg
      841 gtcgccacct ccgcgagacg gatcgtccac tcacccgtgc cgtaggcaag ctcgaggacg
      901 tctccacgag gcgcaaacgc atcgagtgct gaatacactc gattgagctc aagacgccat
      961 ctggcattgt gctcttcgcc caagtcatgg agcccctcgc gacggaacca cttgtcgtac
     1021 tcacgagcgc gagcccgata gtaggcgagt tgctcggcga gaatcggatt cgtgtttgtc
     1081 acagctatcg acgtcgcgag ttgatgcctc gtgtcacccg aaactcggcg gcgacctcgc
     1141 gcgtggccgc ttccgccccg agcgggaaga cgtgcagtgc gtccacgcgc gagacaccgg
     1201 cgagcccggc cgccagctcg cgcgcgagct cgatgccgcc accgcgacgg atctgctcgc
     1261 aggcgggctc ggggatcgtc acgcccggca gcgcgcaggc gcgttctgcc atgcgctcgc
     1321 tgcgcagcaa cgccacactc gcgtagacgc gcacgcccgc gagcatgtcc tcgccgcgcg
     1381 cgaggaatgc ttcgagcctc gggagatcga agaccatctg cgactgcacg aaggttgctc
     1441 ccgcggcgac ctttcgctgc aggagccgca gctcccgatc caggtcttcg gcgaacggat
     1501 tgacgaccgc cccgaccgcc agcgaatgcg ggacgctcca ctccggcgcc ctcgcgatca
     1561 gctcggtcgc ggtcatgtcc gccacatggt cgacgccagc caccggatcg ccctgcatgc
     1621 acagcactcc ctcggcgccg agcgcggcgg cgcccacgac ctgcgactgc aacgcaagcc
     1681 ggttgcggtc gcggcaggaa acgtgcacga tcgtcccgac gccctgctcg cgggcgagag
     1741 ccacagccgc caacggcgac attcgcggct ggccggcgtg gttgtcggtc aacccgacga
     1801 catcgacggt gtcagccaga acagcgatgc gatcactcac ccgctggcca tcggcctcgg
     1861 ccggcagcga aatctccgca gacgtgaggg cagtcacgga caccggcgaa tcatcgcagc
     1921 ccgacgcccc tgagacgcgc cgtggtcgaa ggaaggaagg aaggcaggca gacctcgctt
     1981 tctcgatccg ggcggagccg tgaaatctgg acgatctctc agacggccgc ggcgaacgat
     2041 ggagtgcacc cgtcacgacg gccggcccat tgtcgtcttc gacgcgttcc actagctgat
     2101 ggattgaggc acaaggagaa gtgccagtcc gaagccggtc gcagtcggca gcaggcgaat
     2161 gcaggagcac agctgacggc gcgactggct ggttctctct cggcgccgtc cgatctcacc
     2221 ggcgagccat ag
//
LOCUS       DBNGSH010000897         2629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1471043, whole genome shotgun sequence.
ACCESSION   DBNGSH010000897 DBNGSH010000000
VERSION     DBNGSH010000897.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1471043"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..2572
                     /locus_tag="AC16CY_20605"
     CDS             227..2572
                     /locus_tag="AC16CY_20605"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3356265.1"
                     /translation="MLFWILAVTIPIYAGALYLAYQTTARRLEAGAERDADELAGRLA
                     AGLDGVIRPIEGGIRTVAFQLEEVDPPRSQYEQRIRGILSAWPDVYGSTIAVETANAG
                     GNQQPFAPYLFRRGDGIGFSDLSRDSYAYRDLPWYRRAADSKSPVWSLPYFDAGGGET
                     WMVTYSMPFFRRLPDARRELAGVVTADLDLNWVKSTAAGVTLGSIGMGWLSSPPGAQP
                     FVVPIGDTAGRIAQFDASMQQEAIRRAGEAMLASGVMLAPVPQGLSARPAYLAVRNLQ
                     TLEWRLMLVIPQRELLAGARELLNQQLSLGAIGLALLIAAIWLVAAAVAHPLRALAQA
                     VGGASEEALDFQLPETRRRDEVGVLTEALRRMRSSLQRHIQLRAESLAEQARLEHELE
                     IAASIQQSMLPRRDAATAMPAGVRVAAALLPAKQVGGDLYDYFAIREGEILFAIGDVS
                     DKGIPAALFMARLSALLRVLGAAGELPDRLLTGINARLVDGNEACMFVTVGCGLINVN
                     TGRVRYASAGHEAPLVRHLEGTVRPLGGENGPALGIDTDVDYQLGEGFIAPGDTLVLF
                     TDGVTEAEAEDGSQFGHERLTRLLGEAPDGDPPALVRRIVNTVAAHASGYRATDDLTV
                     MAVGLRPPEVAVRRSDGVVSWVIEPDASAAGIRQAQYWLHAILAAREAGSDRIGDVEL
                     IAEELLTNVVRAAGSREGGVRLSLECALTVSDIMLTFRDDGPPFDPTAVEGPNLDGDI
                     ADRDVGGLGILLVRKLADDCRYSRLDGWNVVEIRLGRTPLN"
BASE COUNT          402 a          869 c          924 g          434 t
ORIGIN      
        1 gcgctgcgcg gcgttccctg gggcgtgaac ttcggccact gagcaaacag cgtctgcgat
       61 ccgatcgcga gcgcaatgaa tgcgatgacg gagatgaatc ggttcatgcc ttccatccta
      121 ttcgaagaag acgctccgtc cgggtcctgg gtcaattgcg gccaactggc cgacaatagc
      181 gggtagagag atcgtgctgg gaagcttccg gacagttcgc ggcaggatgc tgttctggat
      241 cctggccgtg acgattccga tctacgcggg cgccctgtat ctggcgtacc agaccacggc
      301 caggcgtctc gaggccggcg ccgaacgcga tgcggacgag ttggccgggc gtctggcggc
      361 gggcctggac ggcgtcatcc ggccgatcga aggcgggatc cgtactgtgg cgttccagct
      421 cgaggaagtc gatccgcccc ggtcgcagta cgaacagcgc atccgcggca ttctctcggc
      481 ctggcccgac gtgtacgggt ccacgattgc cgtcgagacg gccaacgcgg gcggcaacca
      541 gcagcccttc gccccatacc tgtttcgccg cggagacggg atcgggttct ccgatctctc
      601 gcgcgacagc tacgcgtacc gcgaccttcc ctggtaccgg cgagcggcgg actcgaagag
      661 ccccgtgtgg tcgctgccgt acttcgacgc aggcgggggt gaaacgtgga tggtcaccta
      721 ctcgatgccc ttctttcgca ggctgccgga cgcgcgccgc gagcttgccg gcgtcgtgac
      781 ggcggacctc gatctgaact gggtcaagag cacagcggcc ggggtcaccc tgggctcgat
      841 cggcatgggc tggctctcgt ctccgcccgg cgcccagccg ttcgtcgtgc caatcggcga
      901 caccgcgggc cgcatcgcgc agttcgacgc ctcaatgcag caggaggcga ttcggcgcgc
      961 gggcgaggcg atgctcgcca gcggcgtcat gctcgcacca gtgccgcagg gcctgagcgc
     1021 caggcccgcc tacctggccg tgcgcaacct tcagacgctc gagtggcggc tgatgctggt
     1081 cattccacaa cgggagctgc tggccggtgc gcgggagctc ctgaatcagc agctgtcgct
     1141 cggggcgatc ggcctcgcgc tgctgattgc cgccatctgg ctggtggcgg cggccgtggc
     1201 gcatccgctc cgcgccctgg cgcaggccgt cggcggcgca agcgaagagg ctctcgactt
     1261 tcagctgccg gagacgcgaa ggcgcgacga ggtcggcgtg ctgaccgagg cgctgcgacg
     1321 gatgcggagc tcgctccagc ggcacattca gctgcgtgcc gaaagcctgg cggaacaggc
     1381 gaggctcgag cacgagctcg agatcgcggc cagcatccag cagtcgatgc tgccgaggcg
     1441 cgacgcggcc accgcgatgc cggcgggggt acgcgtggcc gcggccctgc tgccggccaa
     1501 gcaggtcggc ggcgatctgt acgactactt cgcgattcgc gagggggaga tcctcttcgc
     1561 gatcggcgat gtgtcggaca agggtattcc ggccgccctc ttcatggcca ggctgtcagc
     1621 gctgctcagg gtcctcggcg ccgcaggcga gctgccggat cgcctgctca ccgggatcaa
     1681 cgcgagactg gttgacggca acgaggcgtg catgttcgtc accgtcggct gcggcttgat
     1741 caacgtgaac acgggacggg tccgttatgc gagcgccgga cacgaagcgc cgctcgtgcg
     1801 gcatctggag ggaaccgtga gaccgttggg aggcgagaac ggcccggctc tcggcattga
     1861 taccgacgtg gactatcagc tcggcgaagg attcatcgcg ccgggcgata cgctcgtgct
     1921 gttcaccgac ggcgtcacgg aggccgaggc ggaagacggc tcgcagttcg gccacgagcg
     1981 cctgacgagg cttctcggcg aggctcccga cggcgatccg ccggcgctgg tccgccggat
     2041 cgtcaacacc gtcgccgcgc acgcctcggg ttaccgcgcc accgacgatc tcaccgtcat
     2101 ggccgtcggc ctgcgtcccc cggaggtcgc cgttcgccgg tcggatggcg tcgtctcctg
     2161 ggtcatcgag ccggacgcct cggcagcggg cattcgccag gcgcagtact ggctgcacgc
     2221 gatcctggcc gcgcgcgagg ccggctccga tcggatcggg gacgtggagc tgatcgcgga
     2281 agagttgctc accaatgtcg tccgcgccgc cgggtcgcgg gagggcggcg tccgtctttc
     2341 actcgagtgc gccctgacgg tgtccgatat catgctcacg ttccgcgacg acgggccgcc
     2401 gttcgatccg actgctgtcg agggcccgaa tctggacggc gatatcgccg atcgggacgt
     2461 gggcgggctc ggcattctcc tcgtgaggaa gctggccgac gactgccgct actcacgtct
     2521 cgacgggtgg aatgtcgtgg agatccgcct tggccgcaca ccactgaatt gaggtaccca
     2581 tgtcactcaa gatcgtccag catgcgaacc cgccgaccat cgcgctgac
//
LOCUS       DBNGSH010000898         4745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3170315, whole genome shotgun sequence.
ACCESSION   DBNGSH010000898 DBNGSH010000000
VERSION     DBNGSH010000898.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3170315"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="AC16CY_20610"
     CDS             complement(<1..1243)
                     /locus_tag="AC16CY_20610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3356266.1"
                     /translation="MNGTGGVLTAPRARGRVYYGWVVLAVAAAAMVGTLPGRTQGLGL
                     ITEPLLRDVGISRVVFAQINLVATLVGALFCFGIGRLIDRIGSRPVLTATAIALGLTV
                     VVMSQASGVAAMLVLVTLTRGFGQSALSVISLAMVGKWFRRRLTVAMAVYAVAMSIGF
                     MAAFPIVGAVVQASGWRVAWGAIGTCLLLGLAPVAWLFDRSSPEAIGAEVDGGASGDE
                     AAVVAGAQATLGDALRSPAFWVFASASSVYGLVASGIGLLNESILVERGFMPDVYYTA
                     LAVTAITGLAGNFAAGALADRVSLRTILVVAMVVLAGGLTALAHVATQSQVMVQAVAM
                     GIAGGFVTVVFFGFWGRAYGRLHLGRIQGAAQAMTVVASAVGPLFLAVWADRAGSYAS
                     AFYLLAGLAAMLAVAAAFVPMP"
     gene            complement(1317..2006)
                     /locus_tag="AC16CY_20615"
     CDS             complement(1317..2006)
                     /locus_tag="AC16CY_20615"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3356267.1"
                     /translation="MLKVSDVGKSYATPRGPLTVLTGVSFTLSPGDAAAITGPSGSGK
                     SSLLYMLGALEPPSEGAITLSGRNPFTLSPAEVADFRNTDIGFVFQDHCLLPQCSVLE
                     NVLVPTLVARKGGHNSGDDAQRAMACLDQVGLRERLDHRPGELSGGEKQRVAIARALV
                     RQPKLVLCDEPTGNLDQASASTVASLLLDLHARQRNILIVVTHSERLAAQFPIRFEIA
                     DGRLRRAGRSG"
     gene            complement(2000..3313)
                     /locus_tag="AC16CY_20620"
     CDS             complement(2000..3313)
                     /locus_tag="AC16CY_20620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3356268.1"
                     /translation="MRILSITAGAGGMYCGSCFRDNALAAELIARGHDVTLMPVYTPT
                     RTDEENVSRPDVLFGGISVYLQQHSALFRRTPRFVDRLWDSPGVIKAFAGRGVSTDAR
                     LLGDLTISMLEGERGLLKKEFDKLLEWTRDEPRPDVINLPNSLLISMAAPLKRALGAP
                     VCCTLQGEELFVERLQPAYRDETLSLIRRQVPHVDRFVAVSEYCATFMAGFLGIPPAQ
                     MSVVPLGISLTGYTRRDPGGSRDEYRVGYFARIAPEKGLSVLADAYVRFRRRVGTARV
                     KLEVAGYMSPNDAAYLEESRAVLAKAGLADEFAYHGELDRDAKLAFLQGLDVLSVPAT
                     YDEPKGVFLLEAMASGVPVVQPRRGTFTEVVEKTGGGLLVAPDDPEALAEGLHAVWKD
                     RNLAARLAQCAAEGIRAHYTIARSADRILEVYESLGRRAATGASC"
     gene            complement(3356..>4745)
                     /locus_tag="AC16CY_20625"
     CDS             complement(3356..>4745)
                     /locus_tag="AC16CY_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3356269.1"
                     /translation="YNSLEVDEVVHFSTGEPIARVSRANGGLVQRDMRKAQRARDALR
                     DIPIDKLIDMVGRAGDLYMNAALPMGDGTQTADEFVHAQSASTGLPERMCRANMKKNA
                     FVLGEMRSILESLTRGLDFDILSEGHGEERGVPVSYQAQSPVVGLVLPSNSPGVHTLW
                     LPIIPLQIGLVLKPGPQEPWTPYRMASAFFEAGIPSQVISIYPGGADVGASVLDACAR
                     SVVFGGAQTVDRYRGNPGVQAHGPGYSKILLGDDQVDRWEEYLDLMVESVFLNSGRSC
                     INTSGVWASRHTREIADAMAKRLASIRPLPPEHPEASIAAFTVPGVAEGISQSIDADL
                     KAPGVTDVTAKYRNGDRLVKNGKADYLLPTVIHAESPEPEVVKKEFMFPFVTVVECPE
                     SKMIEAIGPTLVCTAITCNEGFKSRLLDAVHVDRLNFGRVPTTQLNWRQPHEGNIVEF
                     LFRARAFQTANL"
BASE COUNT          821 a         1672 c         1551 g          701 t
ORIGIN      
        1 gtggcatcgg cacgaacgcg gccgccacgg cgagcatcgc cgccagtccg gccagcaggt
       61 agaacgcgga cgcgtaggag ccggcgcgat cggcccagac cgcgagaaac agcggcccga
      121 cggccgaggc gaccacggtc atcgcctgcg ccgcgccctg gatccgcccg agatggagcc
      181 ggccgtaggc gcgtccccag aagccgaaga acaccaccgt gacgaaaccg ccggcgatcc
      241 ccatcgctac ggcctgaacc atcacctgcg actgggtcgc gacgtgcgcg agggcggtaa
      301 gcccgcccgc gagcacgacc atcgccacga cgagtattgt gcggagagac acacgatcgg
      361 cgagggcgcc cgccgcgaag tttccggcca ggccggtgat cgccgtgacc gcgagcgccg
      421 tgtagtacac gtccggcatg aatccccgct cgacgagaat cgattcgttg agcaggccga
      481 tgcccgacgc caccaggccg tacaccgagc tggccgacgc gaacacccag aaggccggcg
      541 agcgcagcgc gtcgccgaga gtcgcctgcg ccccggccac gacggctgcc tcgtcgcccg
      601 aggctccgcc atcgacttcc gccccgatcg cctccggcga cgagcgatcg aacagccacg
      661 caaccggtgc gagccccaac agcagacagg tgccgatggc cccccaggcg actcgccagc
      721 ccgaggcctg caccaccgcg ccgacgatcg gaaacgcggc catgaagccg atgctcatcg
      781 ccacggcata gaccgccatc gccacggtca gcctgcgccg gaaccacttg cccaccatcg
      841 ccaggctgat caccgacagc gcgctttgcc cgaacccgcg cgtcaacgtg acgagcacca
      901 gcatcgcggc aacacccgac gcctggctca tgacgaccac ggtcaggccg agggcgattg
      961 cggtcgccgt caggacgggc cggctgccga tccgatcgat cagccgcccg atgccgaagc
     1021 agaacagcgc cccgacgagc gttgccacga ggttgatctg cgcgaagacg acacggctga
     1081 tgccgacgtc ccggaggagc ggctcggtga tgaggccgag cccctgcgtg cggcccggga
     1141 gcgtgccgac catcgccgct gccgcgaccg ccagcacgac ccagccgtag tagacgcggc
     1201 cgcgcgctcg cggggccgtc aacacgccgc cggttccgtt catgtctcct tcccacggtc
     1261 cggctgaagc cggacactac gatcgattcg tttccccgcg tacgtagtgt ccggcttcag
     1321 ccggacctgc cggcgcgccg taagcgcccg tcggcaatct cgaaccgaat cggaaactgc
     1381 gcggcgaggc gctcgctgtg cgtcacgacg atcagaatat tccgctggcg ggcgtgcaga
     1441 tcgagcagga gcgacgcgac cgtcgaggcc gacgcctgat cgaggttgcc cgtcggctcg
     1501 tcgcacagga caagcttggg ctgccgcaca agcgcccgtg caatagccac tcgttgtttc
     1561 tcgccgcccg acaactcgcc cgggcgatgg tcgaggcgct ctcgaagccc gacctggtcc
     1621 agacacgcca tggctcgctg tgcgtcatca cccgagttgt gcccgccctt gcgggcaacg
     1681 agcgtcggca ccagcacgtt ctcgagaacc gagcactgcg gcaacaggca gtgatcctgg
     1741 aagacgaacc cgatgtcggt attcctgaag tcggccacct cggccggcga caacgtgaac
     1801 ggattgcgcc ccgacagcgt gatggcaccc tccgacgggg gctccagcgc gccgagcatg
     1861 tagaggagcg agctcttccc gctgccggac ggcccggtga tcgccgccgc gtccccgggt
     1921 gacagcgtga aggacacgcc ggtcagcacc gtcaggggac cgcgcggcgt ggcatacgac
     1981 ttcccaacgt cagacacctt cagcacgagg cacccgtcgc ggcacgccgt ccgaggcttt
     2041 cgtagacctc cagtatccgg tccgccgatc gggcgatcgt gtagtgcgcg cgaatgccct
     2101 cggcggcgca ctgcgcgagc cgcgccgcga ggtttctgtc cttccacacc gcgtgcaatc
     2161 cttccgcgag ggcctcggga tcgtcgggag cgacgagcag gccgccgccc gtcttctcga
     2221 cgacctcggt gaaagtccct cgtctcggct gcaccacggg gacgccgctc gccatcgcct
     2281 cgagcaggaa gacgcccttg ggctcgtcat acgtcgccgg aacggacagc acgtccaggc
     2341 cctgcaggaa cgcaagcttg gcgtccctgt cgagctcacc gtggtaggcg aactcgtccg
     2401 cgagcccggc tttcgccagc acggcgcgcg actcctccag gtacgccgca tcgtttggcg
     2461 acatgtaacc ggccacttcg agcttcacac gtgccgtacc cacgcggcgc ctgaacctga
     2521 cgtaggcgtc cgccagaacg ctgagccctt tttccggcgc gatgcgcgcg aagtagccga
     2581 ctcgatactc gtcgcgtgag ccgccgggat cgcggcgggt gtagccggtc aggctgatcc
     2641 cgagcgggac gaccgacatc tgcgccggag gaatgccgag gaaccccgcc atgaacgtgg
     2701 cgcagtactc gctgacggcc acgaagcgat cgacgtgagg aacctggcgt cgaatcagcg
     2761 acagcgtctc gtcccggtag gctggctgca gccgctccac gaacagctcc tcgccctgaa
     2821 gcgtgcagca cacgggagct cccagcgccc gtttcagcgg agcggccatc gagatcagca
     2881 gcgagttggg caggttgatg acgtctggtc gcggctcgtc gcgcgtccac tccaggagct
     2941 tgtcgaactc cttcttcagc aggccgcgct cgccctcgag catcgagatg gtcagatcgc
     3001 cgagcagccg cgcgtcggtc gagacgccgc gcccggcgaa cgccttgatg acacccggcg
     3061 agtcccacaa ccgatcgacg aaccggggcg tccgtcgaaa gagggccgaa tgctgctgga
     3121 ggtagacgct gatgcctccg aagaggacgt ccggccggct gacgttttct tcgtcggtgc
     3181 gcgtcggcgt gtacaccggc atcagcgtga catcgtggcc gcgcgcgatc agctcggccg
     3241 caagggcgtt gtcgcggaaa cagctcccgc agtacatccc gcccgcgccc gcagtgatcg
     3301 aaaggattct catgcgagcg ggccgagtgt cctcaagaaa tgactcccga ccccattaca
     3361 gatttgcagt ttgaaacgcg cgcgcgcgga agaggaattc gacgatgttg ccttcgtgcg
     3421 gctggcgcca gttcaactgc gtggtcggca cgcgcccgaa gttcagccga tcgacgtgca
     3481 ccgcgtcgag gaggcgcgac ttgaatccct cgttgcaggt aatggccgtg cagaccagcg
     3541 tgggaccgat ggcctcgatc atcttcgact cggggcactc cacgacggtg acgaacggga
     3601 acatgaattc cttcttcacc acctccggct ccggggactc cgcgtggatg accgtcggga
     3661 gcaggtagtc ggccttcccg ttcttcacga gacggtcgcc attccggtac ttggccgtga
     3721 catcggttac gcccggtgcc ttcaggtccg cgtcgatcga ctgggaaatc ccttccgcca
     3781 cgccaggcac ggtgaacgcc gcgatcgacg cctccggatg ctccggcggg agcggccgta
     3841 tgctggcgag gcgcttcgcc atcgcatcgg cgatctcgcg cgtgtggcgg ctcgcccaca
     3901 cgccggaggt gttgatgcag ctgcggccgc tgttcaggaa gacgctctcg accatcaggt
     3961 cgagatactc ctcccatcga tcaacctggt cgtcgccgag aagaatcttc gagtagccgg
     4021 gcccgtgcgc ctgcacgccc gggttgcccc gatagcgatc gacggtctgg gcgccgccaa
     4081 acaccacgct gcgagcgcac gcgtcgagca cggacgcgcc gacgtcggcg ccgccggggt
     4141 agatcgaaat cacctggctg ggaatccccg cctcgaagaa tgccgacgcc atccggtacg
     4201 gcgtccaggg ctcctgggga ccgggcttga ggacgaggcc gatctggagt gggatgatcg
     4261 ggagccagag cgtgtgcacg cccggggagt tggacggcag caccaggccg acgacagggc
     4321 tctgcgcctg atagctgacg ggcacgccgc gctcctcgcc gtgcccctcg gacaggatgt
     4381 cgaagtcgag cccgcgggtc agcgactcga gaatgctgcg catctcgccg agcacgaagg
     4441 cgttcttctt catgttcgcc cggcacatcc gctccgggag cccggtgctg gccgactgtg
     4501 cgtggacgaa ctcgtcagcc gtctgcgtcc cgtcgcccat cggcagcgcg gcgttcatgt
     4561 acaagtcgcc ggcgcggccg accatgtcga tcagcttgtc gatcgggatg tcacgcagcg
     4621 cgtcccgggc gcgctgcgcc ttccgcatgt cgcgctggac gaggccgccg ttggcgcggc
     4681 tcacgcgggc gatcggctct ccggtcgaaa agtgaacgac ctcgtcaacc tcgaggctgt
     4741 tataa
//
LOCUS       DBNGSH010000899         3091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3339441, whole genome shotgun sequence.
ACCESSION   DBNGSH010000899 DBNGSH010000000
VERSION     DBNGSH010000899.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3339441"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /gene="fusA"
                     /locus_tag="AC16CY_20630"
     CDS             <1..383
                     /gene="fusA"
                     /locus_tag="AC16CY_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525548.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3356270.1"
                     /translation="NELSFKLAGSLAFKDAMTRARPTILEPIMKVEVYLPNEFAGDLM
                     GDLNGRRGRIAGMDTRGTMTVIRAQVPMAEMLTYEQHLTSATGGRGSYHMEYSHSEEV
                     PHSLQTKIIAASKAERGQAVAEEV"
     gene            412..2703
                     /locus_tag="AC16CY_20635"
     CDS             412..2703
                     /locus_tag="AC16CY_20635"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3356271.1"
                     /translation="MAVSPGQRLGPYEVLARIGAGGMGEVYRARDMRLGRDVALKVLP
                     ASAAADPDSLARLEREARTASSLNHPNILTIFDVGELAGTHFITAEFVDGETLRSRLA
                     RERLPVETVIEIAIQMVTGLTVAHEAGLVHRDMKPENVMIRLDGLVKVLDFGLAKPVG
                     PAADDGVTTMATRAGVVVGTTAYMSPEQARGLPLDARTDLFSVGAVMYEMLAGRRAFE
                     GETPSDVLVAVLEHQPEPLGRHRPDVPGDLSRIVDCCLAKDPALRHRTGRDLLADLEP
                     LRAARAAKPDTKAVPSIAVLPFASLSPDPDNEFFADGLMDEVIADLSAVRALRVISRT
                     SAMRYKRTEKDLRTIAHELSVRYVLEGTVRRAAMSLRVTAQLIDAETDSHLWAEKYSG
                     RAEDVFAIQEEISRKIVEALQVRLTDGELRILAERPIENLAAYECYLKARHEMYLVAG
                     DSLDRAQKLVDDGLAIIGENPLLLATKALVLWHHVNQSVGPAERRLDEAARCAAKALA
                     GDPQCDLAIFARGLVAAKRGDLERALPDLRRAAELRPGDGGILAELCRFAFAAGQELR
                     EWVRKPLEDLPRVDPLTPPFRLPVAGWHFSAGRLDRAAGEARRVVELSEAGSPVRIFA
                     AWNLAVAGERDEAKRILRQLAAALTDTPYALYGSLSSFVLAALEGRDEAAVHVTTPLE
                     EAGRWNEYIALLLAEGYAMLARPADAVQWLRTAVTRGFVNYPFLAQRDPLLHAVRNDV
                     GFQALMGEVRTRWNALSAESTEA"
     gene            complement(2715..>3091)
                     /locus_tag="AC16CY_20640"
     CDS             complement(2715..>3091)
                     /locus_tag="AC16CY_20640"
                     /inference="COORDINATES: protein motif:HMM:NF013369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HIR3356272.1"
                     /translation="ARRMVLAALPDPEVVSKLFETLAPRFADRPGGYTRILRVGFRRG
                     DSAEVAHVELVGSEYNPQAKTDKDSAEEAPKKAKGVGERLKRAAQRIRGGKAEGGSKA
                     QAGGQTKGAARKSTTPRKAGGS"
BASE COUNT          491 a         1031 c         1069 g          500 t
ORIGIN      
        1 cgaacgaact gtcgttcaag ctggccgggt cgctcgcctt caaggacgcg atgacgcggg
       61 cgcggcccac catcctcgag ccgatcatga aggtcgaggt ctacctgccc aacgagttcg
      121 cgggagacct gatgggggat ctcaacggcc gccgcggccg tatcgcggga atggacacgc
      181 gcggaacgat gacggtgatc cgggcccagg tgccgatggc ggagatgctg acctacgaac
      241 agcacctgac gtcggccacc ggaggccgcg gctcctatca catggagtac tcccactccg
      301 aggaggtacc ccactcgctt cagacgaaga tcatcgcggc gtcgaaggcc gagcgtgggc
      361 aggcggtggc ggaggaagtc tgagagttcg actccttcgg tagacccccc gatggccgta
      421 agccctggcc aacgtctcgg gccgtatgaa gtactcgcgc gtatcggcgc cggcggcatg
      481 ggggaggtgt accgcgcccg ggacatgagg ctgggccggg acgtcgccct caaggtgctg
      541 cccgcctcag ccgcggccga tccagacagc ctggcgcggc tggaacgcga ggcgcgcacg
      601 gcctcgagcc tcaaccatcc aaacatcctc accatcttcg atgtcggcga gcttgccgga
      661 acccacttca tcacggcgga gttcgtggac ggcgagacgc tgcgctcgcg cctcgcccgg
      721 gagcgcctgc cggtcgagac ggtcatcgag atcgccatcc agatggtcac tggcctgacg
      781 gtcgctcacg aggccggcct cgtccatcgc gacatgaagc ccgagaacgt catgatccgt
      841 ctggacggcc tcgtcaaggt gctcgacttc gggctggcaa agccggtcgg cccggccgcc
      901 gacgatggtg tcacgaccat ggcaacccgc gcgggcgttg tcgtcggcac aacggcgtat
      961 atgtctcccg aacaggctcg tgggctcccg ctcgatgcgc gcaccgacct gttcagcgtg
     1021 ggcgccgtca tgtacgagat gctcgccggg cgacgtgcgt tcgaagggga aacgccgagc
     1081 gacgtcctgg tcgccgttct cgagcaccag ccggagccgc tcggccgtca tcggcccgac
     1141 gtacccggcg atctgtcgcg catcgtggac tgctgtctcg cgaaggatcc ggcacttcgc
     1201 caccggaccg ggcgggatct cttggcggac ctcgagccgc tacgcgccgc gcgcgcggca
     1261 aagccggaca cgaaggcggt gccctcgatc gcggtgctcc cgtttgcgag cctcagcccg
     1321 gatccggaca acgagttctt cgccgacggg ctcatggacg aggtcatcgc ggatctgtcc
     1381 gcggttcgcg ccctccgcgt gatctcccgg acctccgcga tgcggtacaa gcggacggaa
     1441 aaggacttgc ggacgatcgc ccacgagctc agcgtccggt acgtcctcga agggaccgtc
     1501 cgccgcgcag cgatgagcct ccgcgtcacg gcgcagttga tcgacgcaga aaccgattcg
     1561 cacctctggg cggaaaaata ctcgggccgc gccgaagacg tcttcgcgat tcaggaagag
     1621 atctcgagga agatcgtcga ggcgctgcag gtgcgcctca ccgacggcga gttgcggata
     1681 ctggcggaac ggccgatcga gaacctggca gcgtacgagt gctacctgaa ggcgcgtcac
     1741 gagatgtacc tggtagcggg tgattcgctg gaccgcgcgc agaagctggt cgacgacggt
     1801 ctggcgatca tcggcgagaa cccgctgttg ctggcgacca aggcgctcgt gctgtggcac
     1861 cacgtgaacc agtcggtcgg tcccgcggag cggcgtctgg atgaggcggc acgctgtgcc
     1921 gccaaggcgc tcgcgggaga tcctcaatgc gacctcgcca tctttgcgcg cgggctggtt
     1981 gccgccaagc ggggcgatct cgagcgcgcg cttccggacc tccgccgggc cgcggaactc
     2041 aggccgggcg acggcgggat cctcgccgaa ctctgccgct tcgcattcgc ggcggggcag
     2101 gaactgcggg agtgggtgcg caaaccgctc gaggatctgc cgcgagtgga tccgctgacg
     2161 ccgccgttcc gcctccccgt tgccggctgg cacttcagcg ccggccgcct ggatcgcgcg
     2221 gccggagagg ctcgaagggt tgtcgagctg agcgaagcgg ggagcccggt gaggatcttt
     2281 gctgcgtgga acctcgccgt ggcaggcgag cgggacgaag cgaaacggat tctccggcag
     2341 cttgccgcgg cgctcacgga tacgccgtat gcgctgtacg gctcgttgtc cagctttgtc
     2401 ctggcggcgc tggaaggtcg tgatgaagcc gcagttcacg tcacgacgcc gctggaagaa
     2461 gcgggtcgct ggaacgagta catcgcgttg ttgctggccg agggctatgc gatgctcgcg
     2521 cgtccggccg acgccgtcca gtggctgcgg acggcggtga cgcgcggatt cgtcaactac
     2581 ccgtttctcg cccagcgcga tccgctgttg cacgccgttc gcaacgacgt ggggttccag
     2641 gccctgatgg gagaggttcg cacgcgatgg aacgcgctgt cggcggagag cactgaagcg
     2701 tgacggactc cctgctacga gcccccggcc ttcctcggcg tggtggattt cctcgcggcg
     2761 cccttggtct gaccgccggc ctgggccttc gaaccgccct cggccttgcc gccgcggatg
     2821 cgctgagccg cgcgcttcaa acgctccccg acgcccttcg ccttcttcgg cgcttcctcg
     2881 gcactgtcct tgtcggtctt ggcctgcggg ttgtactcgc tgccgacgag ctcgacgtgg
     2941 gccacctcgg ctgaatcacc gcgtcggaaa ccgacgcgaa ggatcctcgt gtagccgccg
     3001 gggcgatccg cgaaacgagg cgcgagcgtc tcgaacagct tgctcacgac ctctgggtcc
     3061 ggcagcgcgg cgagcaccat ccggcgcgcg t
//
LOCUS       DBNGSH010000900         2300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4698774, whole genome shotgun sequence.
ACCESSION   DBNGSH010000900 DBNGSH010000000
VERSION     DBNGSH010000900.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4698774"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="AC16CY_20645"
     CDS             complement(<1..259)
                     /locus_tag="AC16CY_20645"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3356273.1"
                     /translation="MTDPAVDGLAADVYAELRRLAARYVRRERAKSVQATELVHEAYL
                     RLARDKPVRWENRAHFIAIAAISMRRLLVERARARAAAKRGG"
     gene            complement(266..817)
                     /locus_tag="AC16CY_20650"
     CDS             complement(266..817)
                     /locus_tag="AC16CY_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3356274.1"
                     /translation="MRHLTWAELEAGLETIKEAPKDRGRLELIVRRPQVGAREVLDVG
                     ELSHEEGLVGDSWNRRRHPRRPDGSPLTDTQLNVMNARAIALMAQDRSRWPLAGDQLF
                     LDMDISPENLPAGTQLQIGTAVIEVTAEPHTGCGKFVERFGVDAMKFVNSPVGRAHNL
                     RGLNARVVRPGTIRVGDLASKQR"
     gene            complement(814..>2300)
                     /locus_tag="AC16CY_20655"
     CDS             complement(814..>2300)
                     /locus_tag="AC16CY_20655"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3356275.1"
                     /translation="GRRLHAEGRRAVTRVGPFRTWWRSHSVRVRLTLWYVAAIMLVLG
                     VYVFGVYTIVRRNVSESLDERLRADYYWASATVEEGSDGMFMPVPQVDLLLEDESPWV
                     QVWNGDGSELLLANDEARRRPIPEAQALAAQTEDRTATFPTTTVPVRVLSRRSFIGAR
                     PVTIQVARSEAAMRQELRELAIIFVFGLPIAVVIAGLGGYTLARRALAPIEQMTARAR
                     SITAERLSDRLPVSNPDDEMGRLATVFNETLGRLQQSFDQMGRFTADVSHELRTPLTA
                     IRSVGEVGLRGHRDEAAYRGIIGSMLEEVDRLASLVDRLLALSRAETHQATLSLDEVE
                     LARLAEDVVGHLAVLAEEKRQTITIEAGPAPRVRADRLVLRQALINLVDNAIKFTPAG
                     GEIRIRLSDAPLETRVDVVDSGPGVPPEARERIFDRFYRSDGLDAGGSGLGLSLARGA
                     VEALGGHVTLEDTGARGSTFRITLPRKVKSSKVEVQSKLEVRAS"
BASE COUNT          344 a          823 c          751 g          382 t
ORIGIN      
        1 ggccgccgcg cttggcggcc gcgcgcgccc tcgcccgctc cacgagcaga cggcgcatcg
       61 agatggcggc aatggcgatg aaatgcgcgc ggttctccca tcgcaccggc ttgtcgcgtg
      121 cgagccggag ataggcttcg tgaaccagct cggtcgcctg cacgctcttc gcgcgttcgc
      181 ggcggacgta gcgggccgcg agacgccgca gttccgcgta gacgtcggca gccagcccgt
      241 caacggccgg gtccgtcacg cgccctcagc gttgcttgct ggcaaggtcg ccaacccgga
      301 tggtgcccgg ccgcaccacc ctggcattga ggccccgcag gttgtgggcc cggccgacgg
      361 gagagttgac gaacttcatg gcgtccacgc cgaagcgctc gacgaacttc ccgcagccgg
      421 tgtgcggctc ggccgtcacc tcgatcacgg ccgttccgat ctggagctgc gtcccggctg
      481 gaaggttctc cgggctgatg tccatgtcga ggaacagctg atcgcccgcc aacggccacc
      541 gcgatcggtc ctgcgccatc aaggcgatgg cgcgcgcgtt catcacgttc agctgcgtgt
      601 cggtgagtgg cgacccgtcg ggcctgcgcg ggtggcggcg cctgttccag ctgtcgccca
      661 ccaggccttc ttcgtggctc aactcgccga cgtcgaggac ttcccgcgcg ccgacctgcg
      721 gacgcctgac gatcagctcc aggcggcctc tgtccttagg tgcctccttg atcgtctcga
      781 gcccggcctc gagctcggcc catgtcaaat gtctcacgac gccctcactt ccaacttgct
      841 ttgcacttcg acctttgaac tcttcacttt cctcggcaag gtgatgcgga aagtactgcc
      901 ccgtgcgccg gtgtcctcca gtgtcacgtg gccgccaagc gcctcgaccg ctcctctggc
      961 cagcgacaac ccgagcccgc tgccgcctgc atcgagcccg tcgctgcgat agaaccgatc
     1021 gaagatgcgc tcgcgtgcct cgggcggcac gcccggcccc gagtccacga cgtccacacg
     1081 cgtctcgagc ggtgcgtcgg acagccggat cctgatctcg cctcccgcgg gcgtgaactt
     1141 gattgcgttg tccacgaggt tgatgagcgc ctgccgcaag acgagccggt cggcgcgcac
     1201 gcggggagcc gggccagcct cgatcgtgat tgtctggcgc ttctcctccg ccagcacggc
     1261 cagatggccg accacgtcct cggcgaggcg cgcgagctcc acttcgtcga gcgacagcgt
     1321 cgcctgatgc gtttccgcgc gcgacaacgc cagcagccga tcgacgagcg aggcaaggcg
     1381 atcgacttcc tcgagcatgc tgccgatgat gccgcggtag gcggcttcgt cgcgatgccc
     1441 gcgcagcccg acttcgccga cgctccggat cgccgtcagc ggcgtccgta gttcgtgcga
     1501 cacgtcggcc gtgaagcgcc ccatctggtc gaaggactgc tggagccggc cgagggtttc
     1561 gttgaacacc gtcgccagcc ggcccatctc gtcgtccggg ttcgagaccg ggagccgatc
     1621 gctcagccgc tcggcagtga tgctgcgcgc gcgcgcggtc atctgctcga ttggcgcaag
     1681 cgcccgccgc gccagtgtgt agccacccag gccggcaatg acgacggcga tgggaagacc
     1741 gaagacgaag atgatcgcaa gctcccgcag ctcctggcgc atcgctgctt cggagcgcgc
     1801 cacctggatc gtgaccggtc tcgcgccgat gaagctgcgg cggctcagca cgcggaccgg
     1861 tacggtcgtg gtcggaaacg tggcggtccg atcctcggtc tgcgcggcca gcgcctgcgc
     1921 ctcggggatg gggcggcgcc tggcttcgtc gttggccagg agcagctcgc tgccatcgcc
     1981 gttccagact tgcacccagg gcgactcgtc ctcgagcagc agatcgacct ggggcacggg
     2041 catgaacatg ccgtcggatc cttcttcgac ggtcgcggag gcccagtagt agtccgcgcg
     2101 cagcctctca tcgagcgact cggacacgtt tcgcctgacg atggtgtaga cgccgaagac
     2161 gtaaacgccg agaaccagca tgatcgcggc gacgtaccac agcgtcagcc gcacgcgtac
     2221 gctgtgcgac cgccaccacg tcctgaacgg accgacgcgc gtcacggctc gccttccttc
     2281 agcatgaagc cgacgccccg
//
LOCUS       DBNGSH010000901         4255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1641409, whole genome shotgun sequence.
ACCESSION   DBNGSH010000901 DBNGSH010000000
VERSION     DBNGSH010000901.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1641409"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..2534)
                     /locus_tag="AC16CY_20660"
     CDS             complement(177..2534)
                     /locus_tag="AC16CY_20660"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3356276.1"
                     /translation="MGAWLSPPVLERLAGSLLHFVWQGAAIAILAALTLGALRRRSAE
                     SRYAVASAGLLAMVLAPVATFVFYEEAGALAQRLLRSLDDAAAIAAAQAPASGVNLWT
                     RRIVALWAAGVVVCMARLAGGWLLSRGLVRSASAVATPVVQEAMRLARAGLGFDRAVR
                     VLAGTNIGTPVVVGWLRPAILLPASAVTGLDADQLLAILAHELAHIRRQDFLVNALQR
                     AVECVLFYHPAVWWISRRVRVERERCCDDLAVRVCGDRLVYARSLIALEKARSVGPAL
                     AVPAAGDSLTDRVRRILGAGGTSLDWPSAMAALVCVAILVGAGLSRPIFAEPAVLPAP
                     RTSTAPPRTPEPPAETPGPIASPLSAIAAIATAQNARPAAPISSAEPRSPGPQVTQPT
                     ISAARQAAREKLGQLRVEYSADSFARQAAEGDTIAVKLFLAAGMDIDARDQEGFTALA
                     RAARKRQTETAQSLVAAGADPNLAVSKDPAWGSPLAFAAENGDVATMKVLLLGGAKVD
                     LKVGRIGETPLLFAAYAGHRDAAALLLDNKADIEARTTDQSTAILAAVCSRNSDVMRV
                     LGDRGADVNAASFQQRTALHCAAQQHDLEAMEYLVGKGADVNAAAKSRSPLLSALSAP
                     NTDATRASAAAVFLIDRGADVNVTADDSAGSTPLILAVRRQLPDVVGALLARGADPNA
                     KAKNGETALMRALGQALGGTEANASLAMVLIARGADVNLPGRSIVGPAPTPLLLAVQA
                     KNIGLVRALLARGADPNAPTAPSPLSAAAGNEEIVKLLTDAGAKP"
     gene            complement(2534..2965)
                     /locus_tag="AC16CY_20665"
     CDS             complement(2534..2965)
                     /locus_tag="AC16CY_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3356277.1"
                     /translation="MRPRITNPRPTDAELEILNILWQTGPATVRRVHEQLARTKPSQY
                     TTTLKLMQIMAGKGLLERDERERSHVYGPTVGREHAQRQIAAHLMERAFAGSLRSLVV
                     GALGGRRATRGELAELRELIEEHESGRPGARNRNGRNGRAQ"
     gene            complement(3022..4170)
                     /locus_tag="AC16CY_20670"
     CDS             complement(3022..4170)
                     /locus_tag="AC16CY_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3356278.1"
                     /translation="MSAALDPAILLLRDLVAIDSVNPLLVPGARGESDVARRLAEELR
                     ACGLAVEVTDVAPGRPNVVGVLEGRQPGRTLMMCGHTDTVGVAGMLRPFDPEIRGGRL
                     YGRGAQDMKGGVAAMVNAARRVVESGGLPRGRLVVAAVADEEHASRGAEALVTGWRAD
                     AAVVTEPTGLDLAVAHKGFEWVGVETRGRAAHGSRPQEGRDAILRMGRVLSRLEALDG
                     RLQRGAPHALLGTASLHASIIEGGLEMSTYPVAASLKLERRTLPGEPARVGLGEAEAI
                     LEELRAADAEFDARAAPIFSREPYEIDPGSPLPDALMRAAAAVGHAPARVGMTFWTDA
                     AILGAAGIPSVLFGPGGAGLHSQEEYVLIEDVCRCRDVLVELARTFIG"
BASE COUNT          678 a         1506 c         1429 g          642 t
ORIGIN      
        1 ctacggcgtc cgccgggacc tgcaggcctc gctgcgcgcc gacggatacc gcatgcgcgt
       61 ctacgtcccg ttcgggcggg aatggtttcc gtatttcatg cggcgcctcg gggaacgcaa
      121 ggagaacctg tggttcgtga ttcgcgcgct gatacgagaa cgaaggtaag cctccgttag
      181 ggcttcgccc ccgcgtcggt caacaacttc acgatctcct cgttgccagc cgccgcgctc
      241 aaaggactgg gtgctgttgg cgcattcggg tccgcgcccc tggccaggag cgcgcgcacg
      301 aggccgatgt tcttggcctg aacggccaga agcaacgggg tcggcgctgg ccccacgatt
      361 gagcgtcccg gcaggtttac gtcggcaccc ctagcgatca ggaccatggc gaggctcgcg
      421 tttgcttcag tgccgccgag ggcttggccg agggcccgca tcaaagcagt ctcgccattc
      481 ttcgcctttg cgtttggatc ggcgccccgg gccagcagcg cgccgacgac gtctggaagc
      541 tgtcgtcgga ccgccaatat cagcggcgtc gatccggcac tgtcgtctgc ggttacgttg
      601 acgtcggcgc cccgatcgat gagaaacact gcggcggcgc tggcccgggt tgcatctgta
      661 ttgggagcgc tcagcgcgct cagaagaggc gaacgactct tcgctgcggc gttgacgtcg
      721 gcgcccttgc cgaccaggta ctccatggct tcgaggtcgt gttgctgtgc cgcgcaatga
      781 agcgcggtgc gctgctggaa tgaagcggcg ttgacgtcgg ctccccgatc cccaaggacc
      841 ctcatgacgt cgctatttcg agagcagacg gcggccagaa tggcggtgct ctggtccgtg
      901 gtcctggctt caatgtcggc cttgttgtcg agcagcagag ccgccgcgtc gcggtgcccg
      961 gcgtaggcgg caaacagcaa cggtgtctcg ccaatccgac cgaccttgag gtcaaccttc
     1021 gcccccccga gcagcaggac cttcatcgtc gccacgtctc cgttttccgc agcgaaggca
     1081 agggggcttc cccatgccgg atccttgctg accgcgagat tcgggtccgc cccagcggcg
     1141 acgagagact gggcagtctc ggtctgtcgc ttccgcgcgg ctctggcgag ggccgtgaat
     1201 ccctcctgat cgcgcgcgtc gatgtccatc ccagccgcca ggaacagctt gactgcgatg
     1261 gtgtcgcctt cggcagcctg cctcgcgaac gagtcggcgc tgtactccac gcggagctga
     1321 ccgagctttt ccctcgctgc ttgtcgggcc gcggaaatcg tgggctgcgt gacctgcggc
     1381 ccgggcgacc gcggctcggc cgacgagatc ggcgcggccg gccgggcgtt ctgagcggtg
     1441 gcaatggcgg cgatcgcgct taatggagat gcgatgggcc ccggcgtttc cgccggaggc
     1501 tcaggcgtgc gaggcggtgc tgtgctcgtt cgaggagcgg gcagaaccgc cggttccgca
     1561 aatatgggac gcgacaaccc ggcgcccacg aggatcgcca cgcagacgag cgcggccatc
     1621 gcagacggcc agtccaggct tgttcccccc gcgccgagaa tgcgccgcac gcgatcggtc
     1681 agactatcgc cggcggcggg aacggccagg gctggtccga ccgagcgcgc cttctcgagc
     1741 gcgatcaacg atcgggcgta gaccagccga tcgccgcaga cccgaaccgc gaggtcgtcg
     1801 cagcagcgct cgcgttccac gcggactcgt cgagagatcc accacaccgc cggatggtag
     1861 aagagcacac actccacggc gcgctggagg gcgttgacga gaaagtcctg gcgccggatg
     1921 tgcgcgagct cgtgcgcgag aatcgccagg agctgatcgg catccaggcc cgtgaccgcg
     1981 gatgccggca acagaatggc gggccgcaac cagcccacca ccaccggcgt gccgatgttc
     2041 gtccccgcga gcacccgcac tgcgcggtcg aagccgagcc ccgcacgtgc cagccgcatg
     2101 gcctcctgga ccaccggcgt cgcgacggcc gaagccgatc gcaccaggcc gcgcgacagg
     2161 agccatccac ccgccaagcg ggccatacag acgacgacgc cggccgccca caacgcgacg
     2221 atccggcgcg tccagagatt gacgccggag gccggagcct gcgcggcagc gatcgccgcc
     2281 gcatcgtcga ggctgcgcag gagccgctgc gccagagcgc cagcctcttc gtagaagacg
     2341 aaggtcgcga ccggggcgag caccatcgcc agcagccccg ccgacgccac cgcgtagcga
     2401 ctctccgctg agcgtcgtcg cagggcgccg agcgtcagcg cggccagaat cgcaatggcc
     2461 gcgccctgcc agacgaagtg aagcagggaa ccggccagcc gttcgaggac cggcggagac
     2521 agccacgcgc ccatcattgt gccctcccat tccggccgtt ccggttcctt gcgcccggcc
     2581 tgccactttc gtgctcctcg atcaactcac gcagctccgc cagctcgcct ctcgtcgccc
     2641 gccgcccgcc gagggcacca accacgaggc tccgaagcga tccggcgaac gcacgctcca
     2701 tcagatgggc ggcgatctgc cgctgcgcgt gctcccgccc gacggtcgga ccgtagacgt
     2761 gcgaccgctc gcgctcgtca cgctcgagca gacccttgcc cgccatgatc tgcatcagct
     2821 tcaaggtggt cgtgtactgg gagggcttcg tcctggcgag ctgctcatga acacgccgaa
     2881 cggtcgcggg accggtctgc cacaggatgt tcaggatctc gagctccgcg tcggtcggcc
     2941 ggggatttgt aatgcgcggt ctcatagtac gaggctttta ctatgaatag tttcgtatgt
     3001 caaccgcggg gccgccagca tctacccaat gaacgtcctc gcgagctcga cgaggacgtc
     3061 gcggcagcgg cacacgtcct cgatgagcac gtactcctct tgactatgga ggccggcgcc
     3121 tcccggcccg aagagcaccg acgggatccc ggccgcgccg aggatcgccg cgtcggtcca
     3181 gaacgtcatg ccgacgcgcg ccggggcatg gccgacggcg gcggcggcgc gcatcaacgc
     3241 atcgggcaac gggctgccgg gatcgatttc gtacggctcg cggctgaata tgggcgccgc
     3301 acgggcgtcg aattccgcgt cggccgcgcg cagctcttcg aggattgcct cggcctcccc
     3361 cagcccgacc cgggcgggct cgccgggaag agtccgccgt tccaacttga gtgaggccgc
     3421 caccgggtag gtgctcatct cgaggccgcc ttcgatgatc gaggcgtgca gcgatgccgt
     3481 gccgagcagc gcgtgcggcg cgccgcgctg gagccggccg tcgagcgcct cgaggcgcga
     3541 gagcacgcgc cccatgcgga ggatcgcgtc gcgcccctcc tgcgggcggc tgccgtgcgc
     3601 ggcacgtccg cgtgtctcga caccgaccca ctcgaatccc ttgtgtgcga cggcaaggtc
     3661 gagacctgtc ggctcggtca cgaccgccgc atccgctcgc catccggtga cgagcgcctc
     3721 ggcgccccgg ctggcgtgct cctcgtcggc gacggccgca acgaccagac ggccgcgagg
     3781 aaggccgccg ctttcgacca cacgccgcgc cgcgttcacc atcgcggcaa ctcccccctt
     3841 catgtcctgg gcgccgcgcc cgtacaggcg gccgccgcga atctcgggat cgaacggacg
     3901 gagcatgccg gcaacgccga ccgtatcggt gtgcccgcac atcatcagcg tgcgtccggg
     3961 ctgacggcct tcgagcacgc cgacgacgtt gggccgcccc ggagccacgt cggtgacctc
     4021 caccgcaaga ccgcacgcgc ggagctcctc ggccagtcgg cgagccacgt ccgattcgcc
     4081 gcgagcgccg ggcacgagca acgggttgac cgaatcgatc gcgaccaggt cgcgcagcag
     4141 gaggatcgcc gggtccaagg ctgcagacat acgtagataa tcgagtcgtg caaggtacta
     4201 cacccgcgct ggttcgagag atcgccgacg ccgtccggaa cgccggcgga cgcgc
//
LOCUS       DBNGSH010000902        12239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2491411, whole genome shotgun sequence.
ACCESSION   DBNGSH010000902 DBNGSH010000000
VERSION     DBNGSH010000902.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2491411"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..3223)
                     /locus_tag="AC16CY_20675"
     CDS             complement(62..3223)
                     /locus_tag="AC16CY_20675"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3356279.1"
                     /translation="MSIPRLAIQRPVTMFMISAVVTLLGVISLTRLPVDLMPEFEQPQ
                     LNVRTSYPGVGPLEIEELITRPMEQAVGAVPGITRVESSSSEGNSQIRLNFDWGSDLS
                     EAADEVRTRVDRMRNRLPEDADPPTIFKFDSNQLPVMQIGIEGDYDPVTLREVAQNEI
                     APRFERVDGVAAVTVNGGVRREIHVDLSKEKTTALNLSVSTVVQTLRQENQNTPLGEI
                     YQGDSTFLVRSQGQFQSIEDIRNLVVMTRQGVPVYLRDIADVQDTTEQRRSFMRINGR
                     PGIQLQVQKQSGKNTVAVAELVRAEVNRVNQEVPGVKMTITQDNSVFIRRAIDNVQEH
                     AMVGGILVVLIIFAFLRDLRSTLIVCTSIPVSVVGTFALLYFGGFTLNTMTFGGLALG
                     IGMIVDASIVVLENTHRHLHMGKDRMTAAIEGSEEVWSAILASTLTHIAVFVPLLFLS
                     GTASIMFTQLSYVVVFSLAMSLFVAVTIVPVLCSRWLKTPDEEARATGVMAAFYRASE
                     RFLEGMDERYRRAIHVALNHRPTVIGGAAALVVLAVVLYPMVSVELVTQTDEGEVNIN
                     AELPVGTRIERTEEALLRLEEMVREKVPESERITLITNGGGGGNNWSGSGGGGGGSTH
                     RGQIRLLLVPRDQRTRTNEDIAIMLRRELQGALPGVQVRANPSGGNNQIMRFLSGGGT
                     NNNSGGSSRLQVEIRGHDLDDARRVAQDVRAVMEDTPGVADVSVGREEGRPEIAVRVD
                     RPKAAMLGMTVGSVASTIQTNVAGTAAAQFRERGNEYPVVVRLREADRQEISDVGDVL
                     LSTPTGQVVPAKNVLAVNREAGPVQIDRKNMERVTRVNAETEIPLSEAVEAVQERIGQ
                     VQRPPDFSVGFGSEVEEQARSFQQLRMVLILAVLLVYAVMASQYESLRDPFIIMFSVP
                     VAAIGVVLSLMVTGSSFSMQAYIGIIMLAGIVVSNAILLVDYVNTLRRRDGLPIREAV
                     ELGGRTRLRPILMTSIATMLGLVPMAIGVGEGGELQAPLARVVIGGLLTSTVVTLVLV
                     PAVYTLFEEGFSGIFRRTARTAE"
     gene            complement(3307..4797)
                     /locus_tag="AC16CY_20680"
     CDS             complement(3307..4797)
                     /locus_tag="AC16CY_20680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3356280.1"
                     /translation="MKKAVIAAIVLLVGGGAAYYGWQLTSNSSAANPGTNSSGGGRGS
                     QGGGFGGGGFGGGGFGGGGFGNRSGGGRGPMTIELARASIATLDAEITVVGNLIGETT
                     VAVAPRTAGRLEEIYVRLGDRVNRGQRIAKIEDYEIVEQVKQAEAAQEVSLATIRQRE
                     ADLKLAETNVERSRSLYDKKLLPKQTLDDNEARYLASIAQVDLARAQTTQSKARLDEL
                     RINLANTVITSPVTGFVARRAVDPGAFVSQNVPVADVVDITLVRLVANVVEKDLKQLE
                     SGNSANVQVDAYPGETFAGRIARVSPVLDPATRTASIEIEIPNPTFRLKPGMYARVGI
                     TTQTKKNALVLPANAVVDLGGRRGVFQLQNNVAVFRTVQVGTEKGDIVEILAGLKEGE
                     EVISTGAGALRDGDRVLLAGSEDDGSRGRNPQAGPSPRAGSSGQAPSGEAGGDAGRSG
                     GGRSRQAAPGTDGSRSGGAPGGRTGSGDVGGQRGGSLPAPSSAISQ"
     gene            complement(4821..6485)
                     /locus_tag="AC16CY_20685"
     CDS             complement(4821..6485)
                     /locus_tag="AC16CY_20685"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3356281.1"
                     /translation="MRTTCAFLTTLLASASLSPAAAQQAGDQARLAEIARQAAQQFAV
                     ARVDVEQTRPTVPITAPGPSVELTLVEAEARALERNLDLAVERLNPQLQDLQLERLHA
                     TYRPTVSGTFSQRNQVQPPTSQLNGGLIVENNQGAYNTGLTQAVPWGGGDFSVNFTNQ
                     RQETNNSFSNFNPSYNSNLTASYTQPLLRDFRIDSTRQQIRVSTINREVSDIQLRRTI
                     TTTVANVRNSYWELLFASQAVEVARGSLELALKLVDDNRARVEVGTMAPLDVVQAEAE
                     AATRRQTLAQAEATMQTAEIALKRLIVNGTEDDLWRAKITAIDRPEFRPEPLDVEAAV
                     RRSLDNRTDVAQARKTIESNDVTMGYLKNQTLPALDLTANYSAQGVGGTRFERQGLGG
                     SISNIIEGGYGDAMKAIVGRDYPVWQVQLNVSYPLGSSAQEAQYARARVQRNQSVAQL
                     RALELQVAAEVTNAALQVESSLKRFEAATAARELSDTRLRAEESRFEVGLSTNFFVVQ
                     AQRDLQTAQNSELRALLDYRRALVEYERVQDAPAGGGGGITSIQGN"
     gene            complement(6539..6967)
                     /locus_tag="AC16CY_20690"
     CDS             complement(6539..6967)
                     /locus_tag="AC16CY_20690"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3356282.1"
                     /translation="MQFSVRIGWASALLALAVLLAVQAAGQGSKWWQSDKYKRELGLT
                     AEQSRQLEEIFQAAMPNLRVLNETLDQAEAEFERRVQKGDDSAILEQVKRVAASRADL
                     YTARTMMLVRMRKKLTTDQWARFTAMHQAAEKTTQSGRGK"
     gene            complement(7108..8295)
                     /locus_tag="AC16CY_20695"
     CDS             complement(7108..8295)
                     /locus_tag="AC16CY_20695"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR3356283.1"
                     /translation="MTDSPLIFRKGLDRKHEVAAVLAETYHSSLVGRIKESGFRHHAG
                     RLTLHLAREFGFCYGVDRAVDYAYQARQRFPDRTVYLTGEIIHNPHVNDRIRAQGVRF
                     LSDPGESIEALTADDVVILPAFGITIELLETLSKRGCTLVDTTCGSVLNVWKNVKRYA
                     EDGLTSVIHGKYWHEETRATASQAHHGGEGHYLVVLDRREAATACDYIRHGGDRAGFL
                     AAFSHSLSPGFDPDQHLQRIGCANQTTMLSSESLEIGEMFRAAIQDRYGDDTLAARFR
                     SFDTICSATQDRQDAVLALLDEQPLDLMIVLGGYNSSNTCNLARICAGRVPTFHVAEP
                     DGLVSAAEIRHLPVGRKQDVATTGWLPPGEVAVGLTSGASTPDNVLEAVIRRLETAAA
                     GRR"
     gene            complement(8327..8893)
                     /locus_tag="AC16CY_20700"
     CDS             complement(8327..8893)
                     /locus_tag="AC16CY_20700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356284.1"
                     /translation="MSHPHLSDDRLIEIALDLDAASADSAHLQDCEACESRRISLAET
                     LDDVTAAMTVKADEAFSDERLDKQRARILQRVELDGRPARVIVFPNGQTQDAARASMR
                     RARRWATLGGALAASFLIGLAAEHLAHDLSGTRQARPTIAVPQADAGRPSLSDDELFG
                     KVEVAAAQVGPVALMPLDALTPRAWDAR"
     gene            complement(8890..9585)
                     /locus_tag="AC16CY_20705"
     CDS             complement(8890..9585)
                     /locus_tag="AC16CY_20705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3356285.1"
                     /translation="MRTPLQRVTRAGAVSEPVPAGRGKAARHQPGAPSRDSALAARIK
                     ALVQAGDRDRAREQFAELVALHQRRAVRIAYSYLRDAHDADEAVQDAFVKVFAHIASY
                     REEYPFEVWFTRILVNGSLDLRKARARRLRWALPMSAHADAPVTEPAATQPSPEDRLI
                     SSERAGQIAAAVDKLPNRQRTVFTLCHVAEQSTSEISQALGLSEATVRVHLFRAVRKL
                     RKLLAPERGVAVL"
     gene            9771..11369
                     /locus_tag="AC16CY_20710"
     CDS             9771..11369
                     /locus_tag="AC16CY_20710"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HIR3356286.1"
                     /translation="MTIQVERAHGLEALGIVRSGPVHWNLSAAALYEQALRRGEGTLA
                     AEGPLVCRTGQHTGRSPNDKFVVREPSSEKHVHWGNVNRPLDESRFEVLHRDMMAYLQ
                     ETELYVLDAWAGADPAFRLPIRIVNEFAWHNLFARNMFLPENDRARRASHRPEFTVID
                     APNFKADPARHGTRSEVFICVHFGRRLVLIGGTHYAGEIKKSIFTILNYVLPLQGVMS
                     MHCSANIGAAGDTALFFGLSGTGKTTLSSDPARRLIGDDEHGWCDTGVFNFEGGCYAK
                     VIKLSAEAEPQIYETTRRFGTILENVIVDADTRALDLNDASLTENTRGSYPIEFIDNA
                     ELSGRGGHPANVIMLTADAYGVLPPIARLSSEGAMYHFLSGYTARVAGTEKGVTEPKA
                     TFSTCFGAPFLPLNPNVYAKMLGERIARHDARVWLVNTGWTGGPAGVGHRMKIAYTRA
                     MITAALTGQLDRVEYRRHPIFNLDMPASCPGVPDSVLQPRSTWPDADRYDEQARKLAA
                     MFVENFKAFEKDVAPAVTGAGPKA"
     gene            11381..>12239
                     /gene="gatB"
                     /locus_tag="AC16CY_20715"
     CDS             11381..>12239
                     /gene="gatB"
                     /locus_tag="AC16CY_20715"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3356287.1"
                     /translation="MGHANGYEAVIGLEVHAQLRTRTKIFCGCSTQFGSPPNSQVCPV
                     CLGLPGALPVLNHQAVDFAIKAALACGCRIAPTSVFARKNYFYPDLPKGYQISQFDLP
                     ICRGGQLMIPVADGSMHRVGIIRAHLEEDTGKLGHELPGGRPYRGSLIDLNRAGTPLL
                     EIVTHPDLAGAEQAVTFARELRNICRTLGVSECVMQRGHIRFEPNVNVIISDPSGREH
                     ATPIVEVKNLNSFRAIRDAIEYEHRRQVEAWRADGIEMAPGLKRTRGWDDARQVTVLQ
                     RETEEAHDYR"
BASE COUNT         1945 a         4115 c         4045 g         2134 t
ORIGIN      
        1 ctgctgacac tgagcgcttg acgcagggcc ccagggctca ggcctcgggg cccagtctga
       61 tctactcggc cgttctcgcc gttcgccgga agatccccga gaagccttcc tcgaagagcg
      121 tgtacacggc gggcacgagg accagcgtga ccaccgtcga ggtcagcagc ccgccaatca
      181 cgactcgcgc gagcggcgcc tgcagttcgc ctccctcgcc cacgccgatc gccatcggca
      241 ccaggccgag catcgtggcg atcgacgtca tgaggatcgg ccgcaggcgc gtgcggccgc
      301 caagctccac cgcttcacgg atgggaagcc cgtcgcggcg gcgcagtgtg ttgacgtagt
      361 ccacgagaag gatcgcgttg ctcacgacga tcccggccag catgatgatc ccgatatagg
      421 cctgcatgct gaacgacgac ccggtcacca tcaggctgag caccacgccg atggccgcga
      481 cgggaaccga gaacatgatg atgaaggggt cccgcagcga ttcgtactgc gacgccatca
      541 cggcgtagac caggagcacg gccaggatca gcaccatcct cagctgctgg aacgatcgcg
      601 cctgctcctc gacttccgag ccgaatccga cggagaagtc gggggggcgc tggacctggc
      661 cgatccgttc ctggaccgcc tccaccgctt cactgagcgg gatctccgtt tcggcgttga
      721 cccgtgtgac gcgctccatg ttcttccgat cgatctgcac ggggcccgct tcgcggttga
      781 ccgccagcac gttcttcgcc ggcacgacct gtcctgtcgg agtgctgagc aggacgtcgc
      841 cgacgtcgga gatctcctgt cgatcggcct cccgcaggcg gacgacgacc ggatactcgt
      901 tgccccgttc gcggaactgc gccgccgccg taccggcgac gttggtctgg atcgtcgacg
      961 cgacgctgcc gaccgtcatg ccgagcatgg cggccttcgg ccggtccact cgcaccgcga
     1021 tctcgggtcg cccttcctcg cggccgacgc tcacgtccgc aacccccggc gtgtcctcca
     1081 tgacggcgcg gacatcctgc gccacgcggc gcgcgtcgtc gaggtcgtgg ccgcggatct
     1141 cgacctgcag ccggctggaa ccgccgctgt tgttgttcgt tccgcctccg ctgaggaacc
     1201 gcatgatctg gttgttgccg cccgacgggt ttgcgcgcac ctgcacgcct ggcagcgccc
     1261 cctgcaactc gcgccgaagc atgatggcga tgtcctcgtt cgtgcgcgtg cgctggtcgc
     1321 gcgggacgag gaggagccgg atctggccgc gatgcgtgct gccgccgccg ccaccgccgc
     1381 tgcccgacca gttgttgccg ccgcccccgc cgttggtgat gagtgtgatc cgctcgcttt
     1441 cgggaacttt ctcgcgaacc atctcctcca gccgcagcag cgcttcctcg gtccgctcga
     1501 ttcgcgttcc gacggggagc tccgcgttga tgttgacctc gccctcgtcg gtctgcgtga
     1561 ccagttcgac gctcaccatc gggtagagca cgacggcaag gacgacgagc gcggctgctc
     1621 ctccgatcac tgtggggcgg tgattgagcg cgacgtggat cgcccgccgg taccgttcgt
     1681 ccattccttc caggaagcgc tcgctggcgc gatagaaggc ggccatgacg cccgttgcgc
     1741 gcgcctcctc atccggcgtc ttgagccagc gggagcacag aacgggcacg atcgtcaccg
     1801 cgacgaacag ggacatcgcg agcgagaaca cgacgacgta ggagagctgg gtgaacatga
     1861 tgctcgccgt tccgctgagg aacagcagcg gcacgaagac ggcgatgtgc gtgagcgtgg
     1921 aggccagaat cgccgaccat acctcctcgc tcccttcgat ggccgccgtc atgcggtcct
     1981 tgcccatgtg caagtggcga tgcgtgttct cgagcaccac gatcgaggca tcgacgatca
     2041 tgccgatgcc cagcgcgagc ccgccgaagg tcatcgtgtt cagggtgaac ccgccgaagt
     2101 aaagcagcgc gaaagtgccg accaccgaca ccgggatcga cgtgcagacg atcagcgtgg
     2161 agcgcaggtc gcgcaggaag gcaaaaatga tcaacaccac caggatgccg ccgaccatcg
     2221 cgtgctcctg tacgttgtcg atcgcgcgcc ggatgaatac cgagttgtcc tgcgtgatgg
     2281 tcatcttgac cccgggaacc tcctggttca cccgattgac ctccgcacgg acgagttccg
     2341 ccacggccac cgtgttctta ccggactgtt tctggacctg gagctggatg cccggccggc
     2401 cgttgatccg catgaacgag cggcgctgct cggtcgtgtc ctgcacgtcg gcgatgtcgc
     2461 gcaggtacac gggaacgccc tggcgcgtca tcaccaccag gttgcggatg tcctcgatgc
     2521 tctggaactg accctggctg cgaacgagga aggtcgaatc cccctggtag atctcgccga
     2581 gcggcgtgtt ctggttctcc tggcgcagcg tctggacgac cgtgctgacc gagaggttga
     2641 gcgcggtggt cttctccttc gacaggtcca cgtggatctc gcgacggacg ccgccattca
     2701 ccgtcacggc cgccacgccg tccacgcgct cgaagcgcgg ggcgatctcg ttctgggcca
     2761 cctcgcgcag cgtcacgggg tcgtagtccc cctcgatgcc gatctgcatg acgggcagct
     2821 ggttcgaatc gaacttgaag atggtggggg ggtccgcgtc ctcgggaagg cggttgcgca
     2881 tgcggtccac gcgcgttctc acctcgtcgg ccgcttccga caggtctgag ccccaatcga
     2941 agttgagccg gatctggctg ttgccttccg acgagctcga ctcgacgcgc gtgatgcccg
     3001 gcacggcgcc gaccgcctgc tccatcggac gagtgatcaa ctcttcgatc tcgagcgggc
     3061 cgacgcctgg atagctggtc cggacgttga gctgcggctg ctcgaactcc ggcatgaggt
     3121 ccaccggaag ccgcgtcagc gagatcacgc caagcagcgt gacgacggcg ctgatcatga
     3181 acatcgtgac cggacgttgg atcgcgagac gaggaatact catatcggca ctctgtcgtg
     3241 gcgtgacgtg aacgagacgc gcgcttcgag tcgtggcgcg gccgcgtcgg ctggccgcgc
     3301 gccttgctac tggcttatcg cactcgacgg cgccggcagc gaaccgccgc gctgaccgcc
     3361 gacatctccg ctgccggtgc gcccgccggg agcgccgccc gatcgcgatc cgtcagtgcc
     3421 cggcgccgcc tgccggctgc gcccgccgcc ggatcgcccg gcatcgccgc cagcctctcc
     3481 cgacggcgcc tgcccggacg agcctgcgcg cggggacggt ccggcctgcg ggttccggcc
     3541 tcgtgaacca tcatcctcgc tgccggccag caggacgcga tcgccgtcgc gaagggcgcc
     3601 ggcgccggtc gagatgacct cttcgccttc cttgagtccc gccaggatct cgacgatgtc
     3661 gcccttctcg gtacccacct gtacggtccg gaacaccgca acgttgttct gcagctgaaa
     3721 cacgccgcgc cgtccgccca gatcgacgac agcattggcc ggcaggacga gcgcgttctt
     3781 cttcgtctgt gtcgtgatgc ccacgcgcgc gtacatgccg ggcttgagcc ggaacgtcgg
     3841 gttcggaatc tcgatctcga tggacgccgt gcgcgtcgcc ggatcgagca ccggggagac
     3901 gcgcgcgatg cggccggcga aggtctcgcc cggatacgcg tccacctgca cgttcgcgga
     3961 gttgcccgac tcgagctgct tgaggtcctt ttccacgacg ttggcgacaa gcctgacgag
     4021 cgtgatgtcg acgacgtcgg cgaccggaac gttttgcgac acgaacgctc ctggatcgac
     4081 cgcgcggcgc gccacgaagc cggtgacggg cgaggtgatc accgtgttgg ccaggttgat
     4141 ccgcagttcg tcgagacggg ccttggactg cgtggtctgg gcacgcgcca gatccacttg
     4201 agcgatggac gccaggtacc gcgcctcgtt gtcgtcgaga gtctgtttgg gaagcagttt
     4261 cttgtcgtat agacttcgag agcgctcgac gttggtttcc gccagcttca ggtcggcttc
     4321 gcgctggcga atggtggcga gggagacttc ctgtgcggcc tctgcctgct tgacctgctc
     4381 gacgatctcg tagtcttcga tcttggcgat tcgctggcca cggttcacgc gatcgcccag
     4441 acgaacatag atctcctcga gacgcccggc ggtccgcggc gcgaccgcca cggtcgtttc
     4501 gccgatgaga ttcccgacga ccgtgatctc ggcgtcgagc gtcgcgatgc tcgctctcgc
     4561 cagttcgatg gtcatggggc cccggccgcc gccgctgcgg ttcccgaacc cgccgccgcc
     4621 aaagccgccg ccgccgaatc cgccgccgcc gaatccgccg ccctggctgc cgcggccgcc
     4681 accggacgag ttcgtgccgg ggttcgcggc cgacgaattg gacgtcaact gccaaccgta
     4741 gtacgcggcg ccgccaccga cgaggagaac gatggctgca atgacggctt tcttcatgcg
     4801 ttcacgaccg agtctccgtg tcagttcccc tgaatcgagg taatacctcc accgccgccg
     4861 gcgggcgcgt cctgcacccg ttcatattcc accaaagcgc gccggtagtc gagcagggcg
     4921 cgaagctcgg agttctgtgc cgtctgcagg tcccgctggg cctgaacaac gaagaaattc
     4981 gtcgagagac cgacctcgaa ccggctctct tccgcgcgga gccgggtgtc ggacagctcc
     5041 ctggctgccg tggcggcctc gaaccgcttc aggctgctct cgacttgcag cgcggcgttg
     5101 gtcacttcgg cggcgacctg cagctcgagc gcccggagct gcgcgaccga ctggttccgc
     5161 tgcacgcgcg cgcgggcgta ctgcgcttcc tgcgcgctcg agccaagggg ataactgacg
     5221 ttcaactgga cctgccacac gggatagtcg cgaccgacaa ttgccttcat cgcgtccccg
     5281 tagcctcctt caatgatgtt cgagatcgag ccgccaagcc cctggcgttc gaaccgggtg
     5341 ccgccgacgc cctgcgccga gtaattggcc gtcaagtcga gcgccgggag cgtctggttt
     5401 ttcaagtagc ccatggtcac gtcgttgctt tcgatcgtct tgcgcgcctg ggcgacgtcg
     5461 gtgcgattgt cgagcgacct gcgcacggcg gcctcgacgt cgagcggctc gggccggaac
     5521 tccggccgat cgatcgcggt aatcttcgca cgccacaggt cgtcctcggt gccgttgacg
     5581 atgaggcgct tgagcgcgat ctccgcggtt tgcatcgtgg cttccgcctg agcaagcgtc
     5641 tgcctgcgcg tggccgcttc agcttccgcc tggacgacgt cgagcggagc catcgtgccc
     5701 acctccacac gggcccggtt gtcgtcgacg agcttcaacg ccagctcgag cgacccgcgg
     5761 gccacctcaa cggcctggct ggcgaagagc agctcccagt acgagtttcg cacgttggcc
     5821 actgtcgtcg tgatggtcct ccgcagctgt atgtcggaga cctcgcgatt gatcgtcgag
     5881 acgcggatct gctggcgcgt gctgtcgatg cggaagtcgc gcaacagcgg ctgcgtgtag
     5941 ctcgcggtga ggttcgagtt gtagctcggg ttgaaattgg agaaggagtt gttggtttcc
     6001 tgccgctggt tcgtgaagtt caccgagaag tcgccgccgc cccaggggac ggcctgcgta
     6061 agcccggtgt tgtaggcgcc ctggttgttc tcgacgatga ggccgccgtt caactggctc
     6121 gtcggcggct ggacctgatt gcgctgcgag aaggtcccgg acaccgtcgg ccgatacgtc
     6181 gcatgcaggc gctcgagctg gaggtcttgc agttgcgggt tcaggcgctc gacagcgagg
     6241 tcgaggttgc gctcgagcgc gcgcgcctcc gcctcgacga gcgtcagctc gacactcggg
     6301 ccgggcgccg tgatgggaac cgtcggacgg gtctgctcca cgtcaactcg cgccacggca
     6361 aattgctgcg cggcctgacg agcgatctcg gccaggcgcg cctggtcccc cgcctgctgc
     6421 gcggcagccg gcgaaaggct cgcggaggcc agcagagtcg tcaagaaggc acacgtagtt
     6481 ctcatgatgt cttgttaatc ctgttaaatc ctgttaatcg ggatggtcga tatgacggct
     6541 atttgccgcg cccgctctgt gtggtctttt ccgccgcctg atgcatcgcc gtgaacctgg
     6601 cccactggtc cgtcgtgagc ttcttgcgca tgcgaaccag catcatggtt cgggccgtgt
     6661 acaggtcggc tcgagacgct gccacgcgct tcacctgctc caggatggcg ctgtcgtcgc
     6721 ccttctggac ccgccgttcg aactccgcct ctgcctggtc gagcgtctca ttcagaaccc
     6781 gcagattcgg catcgcggcc tgaaagatct cctcgagttg gcggctctgc tcggcggtca
     6841 gtccaagctc gcgcttgtat ttgtccgact gccaccactt ggatccctgg ccggccgcct
     6901 gaacggccag aagtaccgcc aaggcgagca gggcgcttgc ccacccgatc cggacactga
     6961 actgcatggc tattcgtgtt acgaccgcac gtcaagtcgt atttacggcg gcggacgcaa
     7021 ttccatagcc taccacggct taggcttgat tcgacgaggt gtggacgacc ggagcccgcc
     7081 gacggcccga acgggcctcc agaagggcta ccgccggccg gccgccgccg tttccagccg
     7141 acggataacg gcctccagta cgttatcggg agtcgaggcg ccagacgtaa gcccgaccgc
     7201 aacttcccca gggggcaacc atcccgtcgt cgcgacgtcc tgcttcctgc cgaccggcaa
     7261 gtggcggatt tctgccgccg agacgaggcc gtccggctcc gcgacatgga acgtggggac
     7321 gcgcccggcg cagatccggg cgaggttgca ggtgttgctg ctgttgtagc cgcccaggac
     7381 gatcatcaga tcgagcggct gctcgtcgag cagcgcgaga acggcgtcct ggcggtcctg
     7441 ggtcgcgctg cagatggtgt cgaacgatcg aaatcgtgca gccagcgtgt cgtcgccgta
     7501 gcggtcttga atcgccgccc ggaacatctc gccgatttcg agcgactccg aactgagcat
     7561 cgtcgtctgg ttcgcgcaac cgatcctctg gaggtgctgg tcggggtcga agccgggcga
     7621 caggctgtgt gagaaagccg cgaggaagcc ggcccggtcg ccgccatgtc tgatgtagtc
     7681 gcatgccgtc gccgcctcgc gccggtccag gacgaccagg tagtggccct cccctccatg
     7741 atgggcctgc gatgccgtcg cgcgcgtttc ctcgtgccag tacttgccgt ggataacgga
     7801 ggtcaggccg tcctcggcgt agcgcttcac gttcttccag acgttgagca ccgaaccgca
     7861 ggtcgtatcg accagcgtgc agccgcgctt cgagagcgtc tccagcagtt cgatcgtgat
     7921 gccgaacgcc gggaggatga cgacatcgtc tgccgtcagc gcctcgatcg actcgccggg
     7981 atcgctcagg aaccggacgc cctgcgcccg gatccggtca ttgacgtgcg ggttgtggat
     8041 gatctcgccg gtcaggtaca cggtgcggtc gggaaatcgc tgacgcgcct ggtaggcgta
     8101 gtccacggcg cgatcgacgc catagcagaa gccgaactct cgggcgagat gcagcgtgag
     8161 acgcccggcg tgatggcgaa agccggattc tttgatgcgg cctacgaggc tgctgtggta
     8221 cgtctccgcg aggaccgcgg cgacttcgtg cttgcgatcg agccccttgc ggaaaatgag
     8281 agggctgtcg gtcatctggc ccgggcgccg gggttcgcgt cggcgatcaa cgcgcgtccc
     8341 acgcgcgggg cgtcagcgcg tccagcggca tgagcgcaac cggtcccacc tgcgccgcgg
     8401 cgacttcgac cttgccgaac agctcgtcgt cggacaggga cggcctccct gcgtcggcct
     8461 gcggaacggc gatggtcggc cgcgcctgcc gcgttcccga cagatcgtgc gcgagatgct
     8521 ccgcggcgag cccgatgagg aaagacgccg ccagtgcgcc gccaagtgtg gcccagcgtc
     8581 gtgcacggcg catcgacgct cgcgccgcgt cctgtgtctg gccgttcggg aagacgatca
     8641 cgcgagcggg ccgcccatcg agctcgacgc gctgcaggat gcgcgcgcgc tgcttatcga
     8701 gccgctcgtc ggagaacgcc tcatccgctt tcactgtcat cgcagcggtc acgtcgtcca
     8761 gtgtctcggc caggctgatc cggcgcgact cgcaggcctc gcaatcctgc aggtgagcag
     8821 agtcagcaga tgcggcatcg aggtcgagcg cgatctcgat gaggcggtcg tcggacaggt
     8881 gcggatggct cataggacgg cgactcctcg ctcaggcgcg agtagtttcc gaagctttcg
     8941 cacggcgcga aacaaatgaa cacggaccgt cgcctcgctg aggccgagcg cctggctgat
     9001 ctcgctggtg ctctgctcgg cgacatggca caacgtaaag accgtccgct gccgattggg
     9061 gagcttgtcg accgcagcgg cgatctggcc cgcccgctcg ctggaaatca gccggtcctc
     9121 cgggctgggc tgcgtggcag cgggctcggt caccggcgca tccgcatgag ccgacatcgg
     9181 cagggcccat cggagccgcc gcgcccgggc tttgcggagg tccaggctgc cgttcacgag
     9241 gatccgggtg aaccacacct cgaaggggta ctcctcacga tagctcgcga tgtgagcgaa
     9301 cactttcacg aacgcatctt gtacggcctc gtcggcgtcg tgcgcgtccc tcaggtagct
     9361 gtaggcgatg cgtacggcgc gccgctgatg cagcgcaacc aactcggcga actgttcccg
     9421 cgcgcggtcg cgatcgcccg cctgcacgag cgccttgatg cgggccgcca gcgcggaatc
     9481 cctggacggc gcgcctggct ggtggcgcgc ggccttcccg cggccggcgg gaaccggctc
     9541 gctgacggca ccggcacgtg tgacgcgttg caggggcgtt ctcatcgcag attacggccg
     9601 tcccgttctg gtgtttaccg gtgggagcct aacgtgtgtc ccaccaacac tataccccgg
     9661 aacgacccgt gccggcgacg ccgccgcggg cggaccaggc gagttgactg ctttggaccg
     9721 gaaaagtacg atttcctctt tgcgtctctg gccggaggag cggaacacgc atgacgattc
     9781 aggttgagcg ggcgcatgga ctcgaagcgc tcggcatagt ccgatcgggt cccgtccact
     9841 ggaacctgag cgccgcggcg ctctacgaac aggcgctccg ccggggtgaa gggacgctcg
     9901 cggccgaggg gccgctggtg tgccgcaccg gacagcacac cggccgatcg ccgaacgaca
     9961 agttcgtcgt ccgcgagccg tcgagcgaaa agcacgtgca ctggggcaac gtgaaccgtc
    10021 cgctcgacga gtccaggttc gaggtcctgc accgggacat gatggcgtat ctccaggaga
    10081 cggagctcta cgtcctcgac gcgtgggccg gcgccgatcc ggccttccgg ctgcccattc
    10141 gcatcgtcaa cgagttcgcc tggcacaatc tcttcgcccg gaacatgttt ctgcccgaga
    10201 acgatcgggc ccgccgcgcg tcgcaccgcc ctgagttcac cgtgatcgac gcgcccaact
    10261 tcaaggccga cccggcccgg cacggcacgc ggtccgaggt gttcatctgc gtccacttcg
    10321 gccggcggct cgtgctcatc ggcgggaccc actatgcggg cgagatcaag aaatcgatct
    10381 tcacgattct caattacgtc ctgccgctgc agggtgtgat gtcgatgcac tgctcggcca
    10441 acatcggcgc ggccggcgat accgcgctct tcttcggtct ctccgggacc gggaagacga
    10501 cgttgtcgag cgaccctgcc cggcggctca tcggcgacga cgagcacggc tggtgcgaca
    10561 cgggcgtctt caacttcgaa ggcgggtgtt acgccaaagt gatcaagctg tcggcagagg
    10621 cggagccgca gatctacgag accactcgcc ggtttggcac gatcctcgag aacgtcattg
    10681 tcgatgcgga cacgcgggct ctcgacctga acgacgcgtc gctcaccgag aacacgcgcg
    10741 ggtcgtatcc gatcgagttc atcgacaacg cagagttgtc gggccgcggc gggcacccgg
    10801 ccaacgtgat catgctgacg gcggacgcct acggcgtgtt gccgccgatt gcgcggctct
    10861 cgtcggaagg cgcgatgtat cacttcctgt cgggctacac cgcgcgcgtc gcgggaaccg
    10921 aaaaaggcgt gaccgagccc aaggcgacct tcagcacgtg ctttggcgcg ccgttcctgc
    10981 cgctgaatcc caacgtctac gcgaagatgc tcggcgagcg gatcgcgagg cacgacgcgc
    11041 gcgtgtggct cgtgaacacc gggtggacgg gtggaccggc cggcgtcggc caccgtatga
    11101 agattgccta cacgcgcgcg atgatcacgg cggcgctgac gggccaactg gatcgtgtgg
    11161 agtaccgccg gcaccccatc ttcaatctcg acatgcccgc ctcgtgtccc ggcgtgccgg
    11221 actcggtgct ccagccacgg agcacgtggc cggatgccga ccggtacgac gaacaggcgc
    11281 gaaagctggc ggcgatgttc gtcgagaact tcaaggcgtt cgagaaggac gtggctccgg
    11341 cggtcacagg ggctgggccg aaggcgtaag ggccagtcag ttgggacacg cgaacggata
    11401 tgaggccgtc atcggcctcg aagtccacgc gcagttgcgg acgcggacca agatcttctg
    11461 cggctgcagc acgcagttcg gctcgcctcc gaactcgcag gtctgtccgg tgtgcctcgg
    11521 gctgccaggc gccctcccgg tcctcaatca tcaggcggtc gacttcgcga tcaaggcggc
    11581 gctggcgtgc ggctgccgca tcgcgccgac gtcggtgttc gcgcgaaaga actacttcta
    11641 tcccgacctg cccaagggct accagatcag ccagttcgat ctgccgatct gccggggcgg
    11701 gcagctcatg attcccgtcg ccgacgggag catgcatcgc gtgggcatca tccgggcgca
    11761 cctggaggag gacacgggca agctcggcca cgagctgccc gggggccgcc cctatcgggg
    11821 gtcgctcatc gacctcaatc gcgcggggac gccgctgctg gagatcgtca cccatccgga
    11881 cctggctggc gccgagcagg ccgtcacctt tgcccgggag ctgcgcaaca tctgccgcac
    11941 gctcggcgtc agcgagtgcg tgatgcagcg cggccacatc cggttcgagc ccaacgtgaa
    12001 cgtcatcatc tccgacccgt cggggcgcga gcacgccacg ccgatcgtcg aggtcaagaa
    12061 cctcaattcc ttccgggcca tccgggacgc catcgagtac gagcaccggc gccaagtcga
    12121 ggcatggcgg gccgacggga tcgagatggc cccggggctc aagcgcaccc gcggctggga
    12181 cgacgcccgc caggtgacgg tgctgcagcg cgagacggag gaagcgcacg actatcgct
//
LOCUS       DBNGSH010000903         2502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4019919, whole genome shotgun sequence.
ACCESSION   DBNGSH010000903 DBNGSH010000000
VERSION     DBNGSH010000903.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4019919"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="AC16CY_20720"
     CDS             complement(<1..798)
                     /locus_tag="AC16CY_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3356288.1"
                     /translation="MQPQRFCRYELRTTDLNAARAFYADVLGAQFWGPGVSLAPLPER
                     AAAMGAPAHWLGHIGTGDVEAAAGKIVEAGGQRLGPSSKGADGSSLAVLRDPFGAVMA
                     VGSDTPSPPPSAVAWHLLHVKDHERAFATYAGLFGWTATALMDLDLAIGRQQLFAWDD
                     SGESVGSVTDMARLPHVHAQWMFCFRVANLEDARVKVLARGGTMAESTLTPNGDLVAA
                     GEDAQKAAFALLSHEGTKVRPRRHEDITRRTAQPRRRPRRHEDSKAGP"
     gene            complement(877..1845)
                     /locus_tag="AC16CY_20725"
     CDS             complement(877..1845)
                     /locus_tag="AC16CY_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356289.1"
                     /translation="MTSQRIVFSLVAAGTLVGLTAGVGAQDDSQQAPAARRYARIQSG
                     QAQEVVYQALLAQGRRGGGAGGAEAGTDPMTGPVVTNAPYTADAVTTVTQILGDGTRI
                     EQRVTARFYRDSAGRVRREQTVIGLTAEPQTTVTITPEPGTGTAYALDANARTARRVG
                     VGGARIALRTAAGDQLNYFAANEVTWTTGLGLQNSAAAVRLPQPAPGSKPTEESLGTR
                     QIEGVKATGRKSTSTIPAGQIGNDRPIVITDERWESQELKQIVLSRFHDPRTGDVEFQ
                     LTNINRREPPADLFQVPADYTILGGERGGGGGARSGGAGGRGAPAK"
     gene            complement(1870..2358)
                     /locus_tag="AC16CY_20730"
     CDS             complement(1870..2358)
                     /locus_tag="AC16CY_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356290.1"
                     /translation="MQERKHIDPSLSAALRALAEEDGGRSASAAVEARLLREVRSIGR
                     ARQRRGLMAIAAVAAAVFIAVSVPGWHDAPLPVARNIGTGGDTIVNGETTDAFIPLMY
                     ASVPMTNGQIVRLEVSRSALASYGAAPADVPGDRRSATVLADVLVGEDGLARAVRFVR
                     QE"
BASE COUNT          354 a          876 c          825 g          447 t
ORIGIN      
        1 cggacccgcc ttcgaatctt cgtgtcttcg tggcctcctt cgtggttgag ccgtccgtcg
       61 cgtaatgtct tcgtgccttc gtggcctgac cttcgtgcct tcgtggctga gcagcgcgaa
      121 cgcagccttc tgcgcatcct cacccgcggc cacgagatcg ccgttcggcg tgagggtgga
      181 ttcggccatg gtgccgccgc gagcaagaac cttgacgcgc gcgtcttcga ggttggccac
      241 ccggaaacag aacatccact gcgcgtggac gtggggcagt cgcgccatgt ccgtcacgct
      301 tccgacgctc tcgccggagt cgtcccacgc gaagagttgc tgacggccga tggcgaggtc
      361 cagatccatg agcgccgtgg ccgtccagcc gaagaggccg gcgtacgtag caaaggcgcg
      421 ctcatggtct ttcacgtgca gaaggtgcca ggcgacggct gagggtggtg gcgagggggt
      481 gtctgagccg acggccatga cggcgccgaa cgggtcgcgc agtacggcaa gcgacgatcc
      541 gtccgcgccc ttgctgctcg ggcccagtcg ctggccgccc gcctccacga tctttcccgc
      601 cgcggcctcg acgtcgcccg tgccgatgtg accaagccag tgggcgggcg cgcccatcgc
      661 ggcggcgcgt tccgggaggg gcgccagaga gacgcccggt ccccagaact gcgcgccgag
      721 cacgtccgca tagaacgcac gggcggcatt caggtcagtt gtacggagct cgtatcgaca
      781 gaatctctgc ggctgcacgt cactcaaaac tcaaaacgca aaggtcaaaa ctaactcagt
      841 tttgagtctt gagttttgag tcttgagtta ccgtgttcac ttggccggcg ctccacgtcc
      901 cccggcgcca ccggagcgag ctccgccgcc gccgccgcgt tcgccgccca ggatcgtgta
      961 gtcggccggc acctggaaga ggtccgcggg aggctccctg cggttgatgt tggtcagctg
     1021 gaactcgacg tcgcccgtgc gcggatcgtg aaagcgcgag aggacgatct gtttcagctc
     1081 ctgcgactcc catcgctcgt ccgtgatcac gatagggcgg tcgttgccga tctggcctgc
     1141 cgggatcgta ctcgtgctct tgcgtcccgt cgccttcacc ccttcgatct gccgcgtgcc
     1201 cagagactcc tcggtcggtt tgctgccggg cgcgggttgc ggcaggcgga cggccgccgc
     1261 ggagttctgc aagcccaggc cggtcgtcca cgtaacctcg ttggcggcga agtagttcag
     1321 ctgatccccc gccgcagtcc gcaaggcgat ccgtgcgcca cccacaccca ctcgacgcgc
     1381 cgtgcgcgcg ttggcgtcga gcgcgtaggc cgtcccggtg ccgggctcgg gcgtgatggt
     1441 gaccgtcgtc tgcggctcgg cggtgagacc gatgaccgtc tgctcgcgcc gcacgcggcc
     1501 ggcgctgtcg cgatagaacc tcgccgtcac gcgctgctcg atgcgcgtgc cgtccccgag
     1561 tatctgcgtg accgtcgtga cggcgtcagc cgtgtacggc gcgttggtca cgactggtcc
     1621 ggtcatcgga tcggtgcccg cttccgctcc gcccgctccg ccccctcgac ggccttgcgc
     1681 gagcaacgcc tgatagacga cttcctgcgc ctgcccgctt tggatacggg cgtacctcct
     1741 tgcggcaggc gcttgctgac tgtcgtcctg cgcgccgacg cccgccgtca ggccgacgag
     1801 cgtgccggcg gcgacgagag agaagacgat tcgctgcgat gtcatgatgg ctgctcctgc
     1861 gcccacgcac tattcctggc gcacaaatcg gaccgcgcgg gccagtccgt cctcgccgac
     1921 gaggacgtcc gccagcacgg tggcggagcg tcgatcgcct ggcacatccg cgggcgctgc
     1981 gccatacgag gcgagcgccg accgcgacac ttcaagtctg acaatctgtc cgttggtcat
     2041 cggcacgctg gcgtacatca gcgggatgaa cgcgtccgtt gtctcgccgt tcacgatcgt
     2101 gtcgcctccg gtcccgatgt ttcgagcgac cggcagcggc gcgtcgtgcc aaccgggcac
     2161 cgacaccgcg atgaagaccg ccgccgcgac cgccgcgatt gccatcaacc ctcgacgctg
     2221 cctggcgcgg ccgatcgacc gcacctcgcg gagcaggcgc gcttcgacgg ctgccgacgc
     2281 acttcttccg ccgtcttcct cggccagcgc gcgcagcgcg gcgctgagtg atgggtcgat
     2341 gtgtttgcgt tcctgcatgg tcaattctgc cggtccgttt caagccggac acttcgcacg
     2401 cagcccaccg gctgcatgac cgcgagcttc gccgtcagca gcgctttcgc ccggtgcagg
     2461 cgcgatcgga tggtgccgat cgggcagccg acgatctccg cg
//
LOCUS       DBNGSH010000904         2746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_794406, whole genome shotgun sequence.
ACCESSION   DBNGSH010000904 DBNGSH010000000
VERSION     DBNGSH010000904.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_794406"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1132)
                     /locus_tag="AC16CY_20735"
     CDS             complement(95..1132)
                     /locus_tag="AC16CY_20735"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3356291.1"
                     /translation="MTRVLCIATAVSLIACSTAPAQQASPGDGDVVARVGDVVVTAKE
                     IDDHWRSEDPSGHAEAAQKLYDGRRGALDAIVAQILIGQAAKAKGMDPKAFEQAELKA
                     RTKAVGESEVVSFYQSNLNQMQGRSLETMAPAIRQYLQEQRSSEARSSLVADLRKAGP
                     AVRVMIEPPRHQVDLSAEDPSVGSASAPVTLVEFSDFQCPFCQRLAPTIKQVQKTYGD
                     KVRIVWKDFPLTQIHPQAFKAGEAAHCAGDQGKFWEFHDRLFANQDALQPSDLKQHAS
                     ELGLNAATFASCLDSSKHGERVRNGVALGSRLGVNSTPTTYINGRLVAGAQPYEMFVA
                     VIDEELARAKK"
     gene            1206..2105
                     /locus_tag="AC16CY_20740"
     CDS             1206..2105
                     /locus_tag="AC16CY_20740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3356292.1"
                     /translation="MRPLITAALAALAATALTAQQPTFKAGSGVTVSLPTIVLDREGR
                     ISPDLEQDQFTVLDNGKPQPITIFQNSAEPFTVVTMLDFSASMTGNLARLKAATEQFI
                     LRLLPADKGQVGAFSDKIQFSGTFTSDRDDLIGALDDLQFGNPTRLYDAVYASIDELK
                     EVQGRKVVLVFTDGDDTASRLGAGDVADSARDRDIMIYAIGLSSEYFDGISRRSTRPD
                     RGLRKLAEETGGGYFELKKADELAPTFTRVAQELHSLYTIGFVAPVLDGKEHRIEVKM
                     KQPGMTARTRRTYIATQDRLGER"
     gene            2122..2439
                     /gene="cyaY"
                     /locus_tag="AC16CY_20745"
     CDS             2122..2439
                     /gene="cyaY"
                     /locus_tag="AC16CY_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HIR3356293.1"
                     /translation="MALSEQEFRLQADRALEGVQRALLPMADREGFEVELQNGVLQVV
                     FEDPSPAKFVVSPNAPVRQIWVSAMSRSYKLAWNQDALTFQLDGESLGALVERLTQQF
                     LSA"
     gene            complement(2445..>2746)
                     /locus_tag="AC16CY_20750"
     CDS             complement(2445..>2746)
                     /locus_tag="AC16CY_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3356294.1"
                     /translation="ALAHEGRRHGVHATAVCPGFTLSEFHDVTGTRDQVSKLPRWLWM
                     DAATVARQGFEAVMEGTPIYVNGRVNRTIALLVRYLPQAVIVWAGRRLARSYRKT"
BASE COUNT          481 a          931 c          873 g          461 t
ORIGIN      
        1 ccgcttcctg atcaacgtca tcgacagcga gcagcccgcg cagacgccca tcaccgtcgt
       61 caccaactgg acgtcgttgc tgcgttgatc gtcgctactt cttcgctcgc gcgagctcct
      121 cgtcgatgac cgccacgaac atctcgtagg gctgcgcgcc cgcaaccagg cgcccgttga
      181 tgtaagtggt gggtgtcgag ttcacgccga gccgcgatcc cagcgccacg ccgttgcgga
      241 cgcgctcccc gtgcttggag gaatcgaggc acgacgcgaa cgtcgccgcg ttcaagccga
      301 gctcggaggc gtgctgcttc aggtcgctcg gctgaagcgc gtcctgattg gcgaacaggc
      361 gatcgtggaa ctcccagaac ttcccctgat cgccggcgca gtgcgccgcc tcacccgcct
      421 tgaatgcctg cggatgaatc tgcgtcaggg gaaagtcctt ccacacgata cggaccttgt
      481 cgccgtaggt cttttgaacc tgcttgatcg tcggggcgag acgctggcag aaggggcact
      541 ggaaatccga gaactcgacg agcgtgaccg gcgccgacgc gcttccgacc gacgggtcct
      601 ccgccgacag atcaacctga tgccgcggcg gctcgatcat gacgcgcacg gcgggccccg
      661 ccttccgcag gtccgcgacg agcgatgaac gggcctcgct gctccgctgt tcctgcagat
      721 actgccggat ggccggcgcc atcgtctcga gcgaccgccc ctgcatctgg ttgaggttgg
      781 actggtagaa gctcaccacc tcggactcgc cgaccgcctt ggtccgcgcc ttcagttcgg
      841 cttgctcgaa ggccttcgga tccatgcctt tggccttggc ggcctgcccg atcaggatct
      901 gcgcgacgat cgcgtcgagc gccccccgtc gtccgtcgta gagtttctgt gcagcctcgg
      961 cgtggccaga cggatcctcc gaccgccagt ggtcgtcgat ctccttggcc gtgacgacga
     1021 catcgccgac gcgagcaacc acatccccgt cgccggggct cgcctgctgc gcaggggccg
     1081 tcgagcaggc aatcagcgaa acggctgtcg cgatacacag cactctggtc atgtgggaat
     1141 catacgacgg gcagggtccc gccgccgggg tttactcgcc gcagtccggt tgctaagctg
     1201 agtgaatgcg accccttatc acggcggctc tcgcggcgct ggctgcaacg gctcttacgg
     1261 cccaacagcc cacgttcaag gccggcagcg gcgtcacggt gtcgttgccc acgatcgtgc
     1321 tcgaccggga agggcgcatc tcgcccgacc tcgagcagga tcagttcacc gtgctcgaca
     1381 acggcaagcc gcagccgatc acgatcttcc agaacagcgc cgagccgttc accgtggtga
     1441 cgatgctcga cttcagcgcg agcatgacgg gaaacctcgc gcgcctcaaa gccgccaccg
     1501 agcagttcat cctccgcctg ctgcccgcag acaagggcca ggtgggcgcc ttcagcgaca
     1561 agattcagtt cagcggcacc tttaccagcg accgggacga cctgatcgga gcgctcgacg
     1621 atctgcagtt cggcaacccc acgcggctct acgacgccgt gtatgccagc atcgacgagc
     1681 tcaaggaggt ccaggggcgc aaggtggtgc tcgtctttac cgatggcgac gacacggcca
     1741 gccggctcgg cgccggcgac gtggccgaca gcgcgcgcga ccgcgacatc atgatctacg
     1801 ccatcgggct gtcgtctgaa tacttcgacg gcatcagccg gcgatcaacg cggcccgatc
     1861 gcggcttgag gaaactggcc gaagaaacgg gcggcgggta cttcgagctc aagaaggcgg
     1921 acgagctggc gccgaccttt acacgcgtgg cgcaggagct ccacagcctg tacacgatcg
     1981 gcttcgtggc gcccgtgctc gacggcaagg agcacaggat cgaagtgaag atgaaacagc
     2041 cgggcatgac cgcccgcacg cgcaggacct acatcgcaac tcaggacaga ctcggggagc
     2101 gataggcagt tcacagttca catggcgctc tcggaacagg agttccgtct gcaggcggat
     2161 cgcgcgctcg aaggcgtgca gcgcgcgctg ctgccgatgg ccgaccggga aggcttcgaa
     2221 gtggagctgc agaacggcgt gctgcaggtc gtcttcgaag atccctcgcc cgcgaagttc
     2281 gtcgtcagcc cgaacgcgcc cgtccggcag atctgggtgt cggccatgtc gcgcagttac
     2341 aagctcgcct ggaatcagga cgcgttgacg tttcaactgg acggcgaatc gctcggcgcg
     2401 ctcgtcgaac ggctcacgca gcagttcttg agcgcgtaga tcaatcaggt cttcctgtac
     2461 gacctcgcta gacggcggcc cgcccacacg atgaccgcct gcggcaggta tcgcacgagc
     2521 agcgctatcg tgcgattgac ccggccgttg acgtagatcg gcgttccctc catcacggcc
     2581 tcgaaaccct gccgcgccac cgtcgcggcg tccatccaga gccaccgggg aagcttgctg
     2641 acctggtcgc gggtgccggt cacgtcatgg aactcgctca aggtgaagcc cgggcagacg
     2701 gcggtggcgt gcacgccgtg ccgtcgccct tcgtgcgcga gcgcct
//
LOCUS       DBNGSH010000905         2103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1133766, whole genome shotgun sequence.
ACCESSION   DBNGSH010000905 DBNGSH010000000
VERSION     DBNGSH010000905.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1133766"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2000
                     /locus_tag="AC16CY_20755"
     CDS             <1..2000
                     /locus_tag="AC16CY_20755"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR3356295.1"
                     /translation="PKGVSWLGFFYGGNLAGAVAGSLIAGFYLLRVYDMPTATAVAVA
                     LNLIVAVLAVGVAARTPHVVIKSEEPVPATPAAAPEGARLVYVAIALSGLTALGSEVV
                     WTRVLSLLFGATAYTFSLILAVFLFGLGIGSSLGAALARSVKDVRAALGWAQLGLCAS
                     IAWAAYATSESMPYWPINPSISTSPTFTFQLDLMRAIWVMLPGAMLWGASFPLALAGV
                     ARPGQDSARLVGGVYAANTVGAIVGATVTSLGLVGTVGSQLTQQILIGVSAMSGLLML
                     MPPAGEKRPFLSSTPVVVVGIALVAGLLARSVPALPGILVAYGRYAATWVGQNEIVYV
                     GEGVTASVAVSRTPTGVLNYHNAGKVQASSEPQDMKLQRMLGHITTLVPKTPTKVLVI
                     GCGAGVTAGAVSIDPLVKDQTIAEIEPLVPRVVSTHFAQHNFDVVANPKVRIHLDDAR
                     HYLLTTDEKFDAITSDPLDPWVKGAATLYTREFFQVVKDHLNPGGVVTLFVQLYESTD
                     SAVKSEIATFLEAFPNGAVFANTVNGQGYDLVLFGQLEAGKINVDAVQARLDDPANEP
                     IARSLREIGVMSAIDLFGTYAGRKDDMQNWLSDAIINTDRNLKLQYLAGLGLNLYQSD
                     AIYRAMIRDVKYPEALFEGSPETLAALRRKIDLSIGGAPSF"
BASE COUNT          327 a          695 c          710 g          371 t
ORIGIN      
        1 cgccgaaagg cgtgtcctgg ctcggcttct tctacggggg caacctggcg ggagcggtcg
       61 ccggcagcct tatcgccggc ttttatctgc tgcgcgtata cgacatgccg acggcgacgg
      121 ccgtcgcggt ggcgctcaac ctcatcgtgg ccgtgctcgc cgtcggcgtc gccgcgcgta
      181 cgccgcatgt cgtgatcaag tcggaggaac cggttcctgc gacaccggcg gcggcaccgg
      241 aaggcgcgcg actcgtgtac gtggcgatcg ccttgtcggg gttgacggcg ctcggatccg
      301 aggtggtgtg gacgcgtgtg ctgtcgctgc tgttcggcgc cacggcgtac acgttctcgt
      361 tgatcctcgc tgtcttcctc ttcggactcg gcatcggcag cagcctcggc gcggcccttg
      421 cgcgcagcgt gaaggacgta cgagcggcgc tcggctgggc gcagctcggg ttgtgcgcgt
      481 cgatcgcctg ggcggcatat gcgacgagcg agtcgatgcc gtactggccg atcaacccct
      541 ccatctcgac gtcgcccacc ttcaccttcc agctcgatct gatgcgtgcg atctgggtca
      601 tgctgcccgg cgcgatgctc tggggtgcga gctttccgct cgccctcgcc ggagtggcga
      661 ggccgggtca ggattcggcg cggctggtcg gcggcgtcta cgccgccaat actgtgggcg
      721 ccatcgtcgg agccacggtc accagccttg ggctcgtcgg cacggtggga agccagttga
      781 cgcagcagat cctcatcggc gtgtcggcga tgtccgggct gctgatgctg atgcctcccg
      841 ccggcgagaa gcggcccttc ctctcctcga cgccagtcgt tgtcgtcggg atcgccctgg
      901 tggctggtct cctcgcgcgc tcggtgccgg ccctgccggg gattctcgtc gcctacggcc
      961 gatacgcggc cacgtgggtg gggcagaacg agatcgtgta tgtcggggaa ggggttaccg
     1021 cgtcggtggc cgtctcccgc acgccgacgg gcgtgctgaa ctatcacaac gccggaaaag
     1081 tgcaggcgtc gagcgagccg caggacatga agctccagcg catgctcggg cacatcacca
     1141 cgctcgtgcc gaagacgccg acgaaggtgc tcgtcatcgg ctgcggggcc ggcgtgaccg
     1201 cgggcgccgt ctcgatcgat ccgctcgtca aggaccagac gatcgccgag atcgagccgc
     1261 tcgtgccgcg cgttgtctcg acccacttcg ctcagcacaa cttcgacgtg gtggccaatc
     1321 ccaaagtgcg cattcacctg gacgacgcgc gtcactatct gctgacgacc gacgagaagt
     1381 tcgacgcgat cacgtctgac ccgctcgacc cgtgggtcaa gggcgcggcc acgctgtaca
     1441 cgcgcgagtt cttccaggtc gtcaaggatc acctcaatcc gggcggcgtc gtgacgctgt
     1501 tcgtgcagct gtacgagagc accgactcgg cggtgaagag cgagattgcc acgttcctcg
     1561 aggcgttccc gaatggcgcc gtgttcgcca ataccgtcaa cgggcaggga tacgacctgg
     1621 tgctcttcgg acagctggag gccggcaaga tcaacgtgga cgccgttcag gcgcggctcg
     1681 acgacccggc caacgagccg atcgcgcggt cgctgcgcga gatcggcgtc atgtcagcga
     1741 ttgatctgtt cggcacctac gcgggccgca aagacgacat gcagaactgg ctgagcgacg
     1801 ccatcatcaa caccgatcgc aacctcaagc tgcagtacct ggccgggctg ggactcaatc
     1861 tctaccagag cgacgccatc tatcgggcga tgatccgcga cgtgaagtac cccgaggcgc
     1921 tgttcgaggg atcgccggag accctggcgg cgctgcggag gaagatcgac ctctcgattg
     1981 gcggggcgcc gtccttctaa gccccaggcc tcggacctca gacctcagga agccgtcatc
     2041 tcgccgcacc gacgtcaggg ccttgccggt ggacgcggag gccatgaggc catcccggaa
     2101 cac
//
LOCUS       DBNGSH010000906         2730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_625011, whole genome shotgun sequence.
ACCESSION   DBNGSH010000906 DBNGSH010000000
VERSION     DBNGSH010000906.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_625011"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC16CY_20760"
     CDS             <1..377
                     /locus_tag="AC16CY_20760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3356296.1"
                     /translation="VASIYVHYAPYSLRGSHWHVAKDVLLARTIETLERCAPGIRSLV
                     VAAGVVTPAELHTELGLAGGHIFHGELSLDQLFTMRPLLGHAGYDSPIDGLHLCGAGT
                     HPGGFMTGASGRLAAGAVLERR"
     gene            475..2064
                     /locus_tag="AC16CY_20765"
     CDS             475..2064
                     /locus_tag="AC16CY_20765"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3356297.1"
                     /translation="MRPKAVALTLACGAVLGAHVLGAQGRPDAGTASIAGRVLDAATL
                     TPVRRAEVAAVHPSGVRIAATTDDEGRYELERLTTGSWRVTISKPGYFTWQIGQKRPF
                     RAPPPVAVTGGQRLTADVQLTRGGAISGRVYDELGEPVAGLQVRVYRARMLRGRRHAE
                     AVGAADLTDDTGAFRVYGLPPGDYYVGASLRVAPVDSIVETTYAPTYYPGTGNLAEAQ
                     RIKLALGGEATAVFPLLPIRRVRVSGVVATSSGGPANAFLNLESDVTELGMPLGIGGV
                     TRADGTFTIPDVSSGRYNLIASLRGDGPDESGEVPVTVFGDDVTGISITTGKAATLRG
                     SFVADAGISRRLPTGPTVTAITARASGTVLSSGSGLEFELGSLSEPFHLSVDDLPNDW
                     AVKDIFVNGVSAVDGPIDLPRGQAAEARIVLTDRLTEVAGIVDAPTPGDISVVVFPED
                     STKWGFKTRYVQLARTDATGQFRMTGLPPGRQYLAVAADDLEDGEHLDPEFFASMREF
                     AVVLSLGEGEKRAIGLRLVER"
     gene            2061..>2730
                     /locus_tag="AC16CY_20770"
     CDS             2061..>2730
                     /locus_tag="AC16CY_20770"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3356298.1"
                     /translation="MTIRAGIGPYVLVFAVLMSAAVSSQTARSRPQRVDPLTASIRGT
                     VTAAGTGTPVRGAIVHLMADGSGNRLATTDGDGQYELTNLPAGTYRLTVSRAGFSSLQ
                     FGQRRPFETPSQIELSEGERVTANLSLPRAGVIYGRVFDRFGEPVAGTRVQAFRARVV
                     QGQRRMQVMGAGDQTDDTGAYRLYGLAPGDYFVAASVGAVDAVRRDPPIYYPGTPSFS
                     EAQVI"
BASE COUNT          428 a          904 c          960 g          438 t
ORIGIN      
        1 acgtcgcctc gatctacgtg cattacgccc cgtactcgct gcgcggatcg cattggcacg
       61 tggcaaagga cgtgctcctg gctcgcacga tcgagacttt ggagaggtgc gcgccaggaa
      121 tcaggtcact cgtggttgcc gccggcgtcg tcacgcccgc ggaattgcac acggagctcg
      181 gactggcggg aggacacatc tttcacggag agctgtcgct cgatcagctc ttcacgatgc
      241 gtccgctgct cggccacgcg gggtacgaca gcccgatcga tgggctccat ctctgtggag
      301 cgggcacgca cccgggcggc ttcatgacgg gcgcgagcgg ccggctcgcc gcaggggcgg
      361 tattggaacg gcgttgaatg ggtccgccta aaggcggaca ctacgtacca cggtccggct
      421 gaagccggac actacgtaca acggtccgcc tgaaggcgga cactacgtac taccatgaga
      481 ccgaaggccg tcgcgctcac gcttgcctgt ggcgccgtcc ttggcgcgca cgttctcggc
      541 gcacagggac gccccgacgc cggtaccgct tcgatcgccg gccgtgtgct cgacgcggcg
      601 acgctcacgc cggtgcggcg cgccgaggtc gcggcggtcc acccgtcggg cgtccggatc
      661 gcggcgacca ccgatgacga agggcgatat gagctggaga ggctgacgac cgggtcgtgg
      721 cgggtcacca tttcgaagcc tggctacttc acgtggcaga tcgggcagaa gcggccgttt
      781 cgagccccgc ctccagtcgc cgtgaccggc ggccaacgat tgaccgctga tgtgcagctg
      841 acccgcggcg gcgcgatctc ggggcgcgtg tacgacgagt tgggcgagcc tgtcgccggt
      901 cttcaggtgc gcgtgtatcg ggcgcgcatg ttgagaggac gccgtcacgc cgaagcggtg
      961 ggcgccgcgg atctgaccga cgacacgggc gccttccgcg tgtacggtct gccgccgggc
     1021 gactactacg taggcgcgag cctgcgcgtc gcgcccgtgg actcgatcgt cgagacgaca
     1081 tacgcgccga cctattaccc cggtacaggc aatctcgccg aggcgcagcg gatcaagctg
     1141 gcgctcggcg gagaggccac tgccgtcttc ccgctgctgc cgattcggcg cgtgcgcgta
     1201 tccggcgtgg tggcgacctc ctccggcggg ccggccaacg ctttcctgaa cctcgagtcc
     1261 gacgtgaccg agctcggcat gcccctcggc atcggcggcg tcaccagggc ggatggcacc
     1321 ttcacgatcc ccgacgtgtc gtctggacgt tacaacctga tcgcgtcgct tcgtggagac
     1381 ggccccgacg agtccggcga ggtgcccgtc accgtgttcg gcgacgacgt gaccggcatc
     1441 tcgatcacga ccgggaaggc cgcgacgctg cgtggatcgt tcgtggccga tgcgggcata
     1501 tcgcggcgtc tgcccaccgg cccgaccgtc actgcgatta cggcccgcgc ctcaggaacg
     1561 gtcctgtcga gcggctcggg cctggagttc gagctggggt cgctgagcga gcccttccac
     1621 ctgtcggtgg acgacctgcc gaacgactgg gccgtgaagg acattttcgt gaacggcgtc
     1681 agcgccgtcg acggcccgat cgatctcccg cgcggacagg cggccgaggc gcgcatcgtg
     1741 ctcaccgatc gcctgacaga agttgccggg atcgtggatg cgcccacacc aggggacatc
     1801 agcgtcgtcg tgtttccgga ggactcgacg aagtggggat tcaagactcg ctacgtccag
     1861 ctggcaagaa ccgacgcgac cggccagttc cgcatgacgg gcttgccgcc cggccgccaa
     1921 tacctcgcgg tcgcggccga cgacctcgaa gacggcgagc acctggaccc ggagttcttc
     1981 gcgagcatgc gggagttcgc cgtcgtgctg tcgctcggtg agggcgagaa acgcgcaatc
     2041 ggcttgaggc tggtcgaacg atgaccattc gcgcaggtat cgggccgtat gtcctggtgt
     2101 tcgcggtcct gatgtccgcg gccgtgtcga gccagacggc gagatcgcgc cctcagcgag
     2161 tcgatccgct gacagcgagc attcgcggca cggtcaccgc cgcgggcacc ggaacgccgg
     2221 tccgcggagc catcgtgcac ctgatggccg acggctccgg caaccggctc gcgacgaccg
     2281 acggcgacgg ccagtacgag ctgacgaacc tgcccgcagg aacctacagg ctcacggtct
     2341 cccgcgcggg attctcgtcg ctgcagttcg ggcagcgtcg tccgttcgag actccgagcc
     2401 agatcgagct gtcggaggga gagcgcgtga cggcgaacct ctccctgccg cgggcgggcg
     2461 tcatctacgg ccgcgtattc gatcggtttg gcgagccggt ggcaggaacg cgggtgcagg
     2521 cgtttcgcgc gagagtcgtc caggggcagc ggcgcatgca ggtcatgggc gcgggcgacc
     2581 agacggacga caccggcgcc tacaggttgt acggcctggc gcccggggac tacttcgtgg
     2641 cggcaagcgt tggagcagtg gatgccgtca gacgcgaccc gccgatttat tacccgggaa
     2701 cgccgagctt ttccgaagcg caggtgatcg
//
LOCUS       DBNGSH010000907         2937 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_794805, whole genome shotgun sequence.
ACCESSION   DBNGSH010000907 DBNGSH010000000
VERSION     DBNGSH010000907.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_794805"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="AC16CY_20775"
     CDS             complement(<1..315)
                     /locus_tag="AC16CY_20775"
                     /inference="COORDINATES: protein motif:HMM:NF016825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR3356299.1"
                     /translation="MTPERPSSKRALSGRTLDDVLDAFASSAPVPGGGSAAALAGAVG
                     VALLIMVAGLKRRSGDTGADLLADAAERLQPLRQQLTDLVDRDADAYASVMSALRLPK
                     ADE"
     gene            complement(312..1562)
                     /gene="hutI"
                     /locus_tag="AC16CY_20780"
     CDS             complement(312..1562)
                     /gene="hutI"
                     /locus_tag="AC16CY_20780"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR3356300.1"
                     /translation="MISADFLIEHASVIATCAGPAPRRGTAQLEITAVADGTIASKDG
                     LIVYVGPSSSVVGHIEAAPGAVRVDASGCAVVPGFVDPHTHVVFAGDRRSELRDRLAG
                     ATYAQIASAGGGILRTVAATREASPDALVAAALPRLDEMLANGTTTCEVKSGYGLTTE
                     SELEQLRAIRTLDSMHAMDLVATFLGAHEIPREFERARAAYVSLVVDEMIPRVAAERL
                     AAWCDVFCEDGVFTPEEAHAILEAGRRAGLGARIHAEELGPSGGSLVAARVGARSADH
                     LVYCDRAGAEALASADVGAVLLPAAAFYLKLGRFAPARMLIDAGVPVALGTDVNPGGG
                     YSPSMPFAMALACFAMNMTLEESLVAATLNAAWSLDRSDRVGSLEVGKLMDAVVVRGG
                     LTELLRVGASAVRTVVKRGKVVAG"
     gene            complement(1559..1831)
                     /locus_tag="AC16CY_20785"
     CDS             complement(1559..1831)
                     /locus_tag="AC16CY_20785"
                     /inference="COORDINATES: protein motif:HMM:NF014633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR3356301.1"
                     /translation="MDVHVIPRAKKAGLDGVRAGALLVRLHSAPVDGAANKELTAVIA
                     NAFGVSRAAVTIVSGQRSRRKRVHVVGLSVVEADLMVHNLSARARA"
     gene            2070..2144
                     /gene="pqqA"
                     /locus_tag="AC16CY_20790"
     CDS             2070..2144
                     /gene="pqqA"
                     /locus_tag="AC16CY_20790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HIR3356302.1"
                     /translation="MTWTKPEFTVVAVTLEVTAYAGKK"
     gene            2190..>2937
                     /gene="pqqB"
                     /locus_tag="AC16CY_20795"
     CDS             2190..>2937
                     /gene="pqqB"
                     /locus_tag="AC16CY_20795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HIR3356303.1"
                     /translation="MHVVLLGTAAGGGFPQWNCWCRCCRVARADPSLAKPRTQSSAAV
                     SLDGRRWFLLNASPDIRDQLRWIAASDVPVTARHVPVEGILFTDAEIDHTLGLVLLRE
                     AGCLRVHATPAVASILEHDSRLLSTTRAFSDVPVTALSLNERVNVAYRDGSASGLSVE
                     AFAVPAGPPRFASREEEGHTVGLMLRDETGGAACAFVPGCGGLDAALLDRLAEADGLL
                     FDGTFWSDDELIALGIGARTAREIDHLPIGG"
BASE COUNT          469 a          998 c          993 g          477 t
ORIGIN      
        1 ctcatcggcc ttcggcagcc gcagggcgct catcacggag gcgtacgcat cggcgtcgcg
       61 gtcaacaaga tcagtcaact gctggcgaag gggctgaagc cgttcggctg cgtccgcgag
      121 caggtcagcc cctgtgtcgc cgcttcgcct cttcaggccg gcgaccatga ttagcagggc
      181 cacgccgacg gcaccggcca gggcggccgc tgaccctcct cccgggaccg gcgcgctcga
      241 cgcaaaggcg tcgagcacgt cgtcgagcgt gcggccggac agcgctcgct tggacgaggg
      301 tcgctcgggt gtcatccggc cacgaccttg ccgcgtttga cgacggtccg taccgcggac
      361 gcaccgactc gaagcagctc ggtcaagccg cctcggacga cgaccgcgtc catcaacttg
      421 ccaacctcaa ggctgccgac ccgatcggac ctgtccagcg accacgcggc gttgagcgtc
      481 gccgccacca gcgattcctc gagcgtcatg ttcatcgcga aacaggccag cgccatcgca
      541 aacggcatcg acggcgagta gccgccgccg ggattgacgt cggtcccgag cgccaccggc
      601 acaccggcgt cgatcaacat cctggccggc gcgaagcggc ccagcttgag atagaaggcg
      661 gccgccggca gcagcaccgc gccgacatcg gcggacgcga gtgcctcggc accggcgcgg
      721 tcgcagtaga ccaggtgatc ggccgaccgc gcgccgacgc gcgcagcgac cagactgcct
      781 ccgctcggtc caagctcctc ggcgtggatg cgtgcgccca ggcccgcacg cctgcccgcc
      841 tccagtatcg cgtgcgcctc ttcgggggtg aacacgccgt cctcgcagaa gacgtcgcac
      901 catgccgcca gccgttcggc ggcgactcgc gggatcatct catcgacgac gagcgacacg
      961 taagccgcgc gggcacgttc gaactctcgc gggatctcgt gcgcgccaag gaaggtcgcc
     1021 acgaggtcca tcgcgtgcat cgagtcgagc gtccggatcg cgcgcagctg ttccagctcc
     1081 gattccgtcg tgaggccgta cccgctcttg acctcgcacg tcgtcgtacc gttggcgagc
     1141 atttcgtcga ggcgcggcaa cgccgccgcc acgagggcgt ccggcgaggc ctcgcgcgtg
     1201 gcggcgaccg tgcgcagaat tccgccgcct gccgacgcga tctgggcgta ggtggcgccc
     1261 gcgaggcgat cgcgaagctc cgatcggcga tcgccggcga agacgacgtg cgtgtgagga
     1321 tcgacgaagc caggaacgac ggcgcaaccc gaggcatcga cgcgcaccgc tcctggcgcg
     1381 gcctcgatgt ggcccacgac gctggaagaa gggccgacgt agacgatgag cccgtctttc
     1441 gaggcgatcg tcccatcggc aacagccgtg atctcgagct gggcggtgcc gcggcgcggt
     1501 gccggaccgg cgcaggtggc gatcacgctc gcgtgttcga tcaggaagtc ggcggaaatc
     1561 acgctcgggc ccgggcgctc aggttgtgga ccatcaagtc ggcctccacg acggacaggc
     1621 ccacgacgtg cactctcttt ctgcgcgacc gctggccgga gacgatcgtg accgcggccc
     1681 tggacacgcc gaacgcgttc gcgatcacag cggtcagttc cttgttggcg gctccatcga
     1741 cgggcgcaga gtggagccgg accagcagcg cgcccgcccg tacgccatcg agacctgcct
     1801 tcttcgctcg tggaatgacg tgcacgtcca tggcaactcc ctgtggcgtt gggcagatca
     1861 tgacgtgtgg tgacacgagg tgtgcattct agcggacggt agaaggaccc tgccgcgtcc
     1921 agggtggccc agtggaagtc tttacgtgcc agttagatag cggtggcaag atctgagctg
     1981 ttgcgcccgg ggtagccaga tccatataat tcgaggtgct atagcaactg gggccccagg
     2041 ggggtacccg tcactggaag gagtcttcaa tgacgtggac caaacccgaa ttcacggttg
     2101 tggccgtgac tttggaagtt accgcgtacg ccgggaagaa gtagatccca aataggcggg
     2161 gctcgtgagg gccccgccta ttcttttgcg tgcacgtcgt tctgctcggc accgccgctg
     2221 gcggaggatt cccccagtgg aattgttggt gtcggtgctg ccgggttgca cgcgccgatc
     2281 cctccctcgc gaagccccgc acacagtcgt ccgccgcggt gagcctcgac ggtcggcgat
     2341 ggttcctgct caacgcgtcc cctgacattc gcgaccaact gcgctggata gccgcatccg
     2401 acgttcctgt aactgcgcgt cacgtgccgg tcgaagggat cctcttcacc gacgccgaga
     2461 tcgatcacac gctgggcctc gtgctgctcc gggaagcggg atgccttcgc gtccacgcga
     2521 ctccggcggt cgcctcgatt ctcgaacacg attcccggct gctctcgacc acccgcgcgt
     2581 tcagcgacgt gccggtcacg gcgctatcct tgaacgagcg cgtcaacgtg gcctaccgtg
     2641 acggttctgc gagcggtctg tcggtggagg cgtttgccgt cccggccggc cctccgcgat
     2701 ttgcctcgcg ggaagaggag gggcacaccg tcggcctcat gctccgcgat gaaacgggcg
     2761 gggcggcctg tgcgttcgtg ccggggtgcg gcggcctcga cgccgcgctt ctcgatcggc
     2821 tggcggaggc tgacggcctg ctgttcgacg ggacgttctg gtccgacgac gagctgattg
     2881 cgctcgggat cggggcacgc acggctcgcg agatcgatca ccttccgatc ggagggg
//
LOCUS       DBNGSH010000908         5173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_115643, whole genome shotgun sequence.
ACCESSION   DBNGSH010000908 DBNGSH010000000
VERSION     DBNGSH010000908.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_115643"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="ispE"
                     /locus_tag="AC16CY_20800"
     CDS             complement(<1..546)
                     /gene="ispE"
                     /locus_tag="AC16CY_20800"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3356304.1"
                     /translation="MKAITVRAHAKINLDLRVLGMRPDGFHELRTVFQAVALHDSVEC
                     IPRPGPLAIECETAGVPLDRSNLAWRAAEALWRALRRDGEVRDILIRLHKRIPLQGGL
                     GGGSTDAAATLLALCRVWRVPVRPSQLVDVASVLGADVPFFRSGGTALGLGRGDEIYP
                     LADLPRHWVVLLVPGFGVATAD"
     gene            complement(576..1214)
                     /locus_tag="AC16CY_20805"
     CDS             complement(576..1214)
                     /locus_tag="AC16CY_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIR3356305.1"
                     /translation="MRTLTAELGLRGRAGRQRLRGRLIAGFERPDSMRLEAVAPLGAP
                     VFILASRGTTAVLLLTREDRVVRGESAEAILGALTGVSLSPADLQAILDGCVVPEPRA
                     TGGRQHADGSASIDLAGGARLYLRRADTWQVRAARRAGWQVEYPAWEGSFPASVRLQS
                     DSPSVQVDVTATLTQIETNAGIRPDAFSIDVPSSARPLSLAELRDAGPLGDK"
     gene            complement(1358..2782)
                     /locus_tag="AC16CY_20810"
     CDS             complement(1358..2782)
                     /locus_tag="AC16CY_20810"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356306.1"
                     /translation="MPNTLARSFVVVGALLSVLGPSAALAQGTSEAYLAFLMARRLEA
                     EGDNSGALAALKRAADMDPKSAEVRAEMAGFHLRRNERPQAEAEAKAALAIDADNAGA
                     NRTLGAINAAAYESSLGKAPAAQSATYLRDAIMYLERAAAQSSVTADVRLFDTLGRLY
                     LQNGDTRKAIDAFTKVVSQNPDSAEGRLWLAQSYAANKDLKSAIETLDEIVEYAPRVA
                     AILGQYQEEAGLLADAVESYTLALSVRTRDREVKVRRIVVLLDLKEYGRAAGFAAEAR
                     KQHPEDVRFVRLQARALFDGGDRTGAIALLEQVTKASPKDTGTLYALADIYVDAGRGN
                     DAERTLRQIIASEPANANALNYLGYLLALRGERLDEAVSLVQQALKAEPENGAYLDSL
                     GWAYFRRGDLDEAEKYLAAAAARLPENSEVQDHLGDLFARRGRMAEAVDAWTRALKGD
                     SQDVDKATIEKKISNAKGKMQNAK"
     gene            2840..3013
                     /locus_tag="AC16CY_20815"
     CDS             2840..3013
                     /locus_tag="AC16CY_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR3356307.1"
                     /translation="MPVDQELLEILACPNCKTPVRLVNDSQGLKCDTCRRVYPIKDDI
                     PVMLIDEATIDAD"
     gene            3140..4333
                     /locus_tag="AC16CY_20820"
     CDS             3140..4333
                     /locus_tag="AC16CY_20820"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3356308.1"
                     /translation="MTATTLDIQHPRSAPGLDRLSNIFLLVFAASVQLSIVAGEAALA
                     ALLLVWGVGLVQRRTWPAAPMFFPALLAFAGVTLISSAFSIDPLASLIDSRQLLLFLI
                     VPAVYDLARGQRATTVTDVIITVGAATAIYGIVQYGMLHYDNLGRRPGGTLGQYMTFS
                     GTLMLVLGAVAARLVLGTRGRMWPALVMPALVVALSLTLTRSAWVGACVSVALIFILK
                     DLRLLGVLPVVGALLFAFAPDSVTNRMMSTFDLRDPSNRDRLAMMRSGSAMVRDHPLT
                     GVGPEMIPRVYAQYRDPDAVQATNRHLHNVPMQIAAERGLPALAVWIAFVAVLTFGLF
                     RLFRAGRDPALAGAALAAIAGMLSAGFFEYNFGDSEFLMLFLVIVTLPFAASRPDDAA
                     ANPHA"
     gene            4308..>5173
                     /locus_tag="AC16CY_20825"
     CDS             4308..>5173
                     /locus_tag="AC16CY_20825"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HIR3356309.1"
                     /translation="MLLPTLTRDRAREIMSRAVGAGVLVIGDAMVDKFIVGRVSRISP
                     EAPVPVVAFDHDLHRMGGAANVASNIVALGGQATIVAVTGRDEAAATLTEACREAGIA
                     PSLVSDASRGTTTKVRVVTDRNQQVARIDYEVDDEIAGEIESRVIAEIGKHAPRASAI
                     VLSDYLKGCVTRQVVQTALAAAARHGVPLLVDPKIPHLPYYAGASIVTPNHHEAEAAT
                     GVRVRSEDDARRAARLFRERAGCRDVVMTRGDQGIWLLSDRVEGHLPAAAREVADVTG
                     AGDTVVATLALAL"
BASE COUNT          788 a         1799 c         1750 g          836 t
ORIGIN      
        1 gtccgcggtt gccacgccga agcctggcac gagcagcacg acccagtggc gcggcaagtc
       61 agcgagaggg taaatctcgt cgccgcgccc gaggccgaga gcggtcccgc ccgaccgaaa
      121 gaacggcacg tcggcgccaa gcacggaggc gacgtcgacg agctggctgg gacgtaccgg
      181 cacacgccac acccggcaca gtgccagcag cgtcgccgcg gcgtcggtgc tgccgccgcc
      241 gagccctccc tgcaggggga tgcgcttgtg aagccggatc aggatgtccc tgacttcccc
      301 gtcgcgcctg agcgcgcgcc acaacgcctc cgccgcccgc cacgcaaggt tcgaacggtc
      361 gagaggaaca ccggccgttt cgcactcgat ggccagcggc cccggccgcg ggatgcactc
      421 gacgctgtcg tgcagcgcca ccgcctggaa gaccgtccgg agctcgtgaa aaccgtccgg
      481 cctcatgccg agcacgcgca gatccagatt aatctttgcg tgagcgcgca cggtaatcgc
      541 cttcacggac gccgtcccgg agcgtggacc aggcatcact tgtcgccgag cggtccagcg
      601 tcgcggagct ccgcgagcga cagcggacgc gcgctcgagg gaacgtcgat cgaaaacgcg
      661 tccggcctga tgcccgcgtt tgtttcgatt tgcgtgagcg tcgcggtcac gtccacctga
      721 accgaagggg aatcggactg cagtcgaacc gacgcgggaa acgagccctc ccacgcgggg
      781 tactcgacct gccacccggc gcggcgtgcg gcgcgaacct gccacgtatc ggcgcggcgg
      841 aggtagagcc tcgcgccgcc ggcgaggtcg atcgatgccg atccatcggc gtgctggcgg
      901 ccacccgtcg cccgtggttc gggcacgacg cagccgtcga gaatggcttg aagatcggcg
      961 ggcgacagcg acacaccggt cagcgccccg aggatcgcct cggccgactc gccccggacg
     1021 actcggtcct cgcgagtcag gagaagaacg gcggtcgttc cccgcgacgc cagaatgaag
     1081 acgggtgccc cgagcggagc gaccgcctcg agcctcatcg agtccggccg ttcgaacccg
     1141 gcaatcaagc gcccgcgaag acgctggcgg ccggcgcggc cgcgcaaacc gagctcggcg
     1201 gtcagcgtgc gcacgccgcg gcaggccgca gcgacctgcg cgtggatctc ctcgtatttc
     1261 ggaaggggcg aacccggatc agtcgggacg gtgaatcgtt tcggtgcgca cgaagcggaa
     1321 gcgacacacg caatgcaaaa tgcaagatgc aggtgtttca ttttgcattt tgcatcttgc
     1381 cttttgcgtt actgatcttc ttctcgatcg tcgccttgtc cacgtcctga ctgtcgccct
     1441 tgagcgcgcg cgtccaggcg tcgacagctt cggccatgcg cccccggcgg gcgaacagat
     1501 cgccgaggtg atcctggacc tcggagttct cgggaagccg ggcggcggcg gccgccagat
     1561 acttctccgc ctcgtccagg tcgccgcggc ggaagtaggc ccatcccagg ctgtcgaggt
     1621 acgcgccgtt ctccggctcg gccttgagcg cctgctggac gagactgacc gcctcgtcga
     1681 gccgttcgcc gcgcagcgcc aggaggtagc ccagatagtt gagcgcattc gcattggccg
     1741 gttccgaggc aatgatctgg cggagcgttc gctcggcgtc gttgccccgg ccggcgtcga
     1801 cgtagatgtc ggccaacgcg tagagcgtgc cggtgtcctt cggcgacgcc ttcgtcacct
     1861 gctcgagcag cgcgatcgct ccggtccggt cgccgccgtc gaacagcgcg cgagcctgga
     1921 gccggacgaa ccggacgtcc tcgggatgct gcttccgcgc ctcggccgca aacccggccg
     1981 cgcgcccgta ctctttcagg tcgagcagca cgacgatgcg cctgaccttc acttcgcgat
     2041 ctctcgtgcg aaccgacagc gcgagggtgt agctctcgac ggcgtcggcc agcagccccg
     2101 cctcttcctg gtactggccg aggatcgccg cgacgcgcgg cgcgtactcg acgatctcgt
     2161 cgagcgtctc gatcgcgctc ttcagatcct tgttcgcggc gtaggactgc gccagccaga
     2221 gccggccctc cgcggagtcc ggattctgac tgaccacctt cgtgaacgca tcgatcgcct
     2281 tgcgcgtatc cccgttctgc agatagaggc ggcccagcgt gtcgaagagc ctcacatcgg
     2341 ccgtcacgct cgattgtgcg gccgcccgct cgagatacat gatggcgtcg cgcaggtagg
     2401 tggccgactg cgcggccggc gccttgccca agctcgactc gtacgcggcg gcgttgatgg
     2461 cgccgagcgt gcgattggcg ccggcgttgt ccgcatcgat cgccagggcg gccttggcct
     2521 cggcttctgc ctgcggccgc tcgttgcgac gcaggtggaa ccccgccatc tcggcgcgaa
     2581 cctccgccga cttgggatcc atgtcggcgg cgcgcttgag cgccgccagc gccccgctgt
     2641 tgtccccttc agcttcaaga cgcctcgcca tcaggaacgc gaggtacgcc tccgaggtcc
     2701 cctgcgcgag cgcggccgac ggtccgagaa cgctcagcag ggcgccgacg accacaaacg
     2761 accgggcgag ggtgttgggc atagctaagc gatcttagcc caggatgtta tagttcgagc
     2821 ggatcaggcc ggagacggta tgccagttga ccaggaactg ctcgaaatcc tcgcgtgccc
     2881 caactgcaag acccccgtcc ggctcgtcaa cgacagccag ggcctcaaat gcgacacgtg
     2941 ccgccgggtc tatccgatca aggacgacat cccggtcatg ctgatcgacg aggccaccat
     3001 cgacgccgac tagtggtatg gccttcgcgg attacgagcg gccgctccgt tgtcgcggcc
     3061 ggaaaatcgc ggtcggagct cgttgttcgc ggtactcttc cagtggatac ggtccagtag
     3121 tgcgaccgct ggtgcgacca tgacggccac caccctcgac atccagcacc cacggagcgc
     3181 cccgggcctc gaccggctgt cgaacatctt cctgctcgtg ttcgccgcct cggtccagtt
     3241 gtccatcgtc gccggcgagg ccgccctggc ggcactgctg ctggtctggg gggtcggcct
     3301 cgttcagcgc cggacctggc cagcggcccc gatgttcttt cccgcgctcc tggcctttgc
     3361 cggagtcacg ttgatttcct cggccttctc gatcgatccg ctcgccagcc tgatcgacag
     3421 ccgtcaactg ctcctcttcc tgatcgtgcc cgcggtgtac gacctcgcgc gcggccagcg
     3481 cgccacgacg gtcaccgacg tcatcatcac ggtcggcgcg gcaaccgcca tctacggcat
     3541 cgtccagtac gggatgctcc actacgacaa cctgggccgc cgcccgggcg gaacgctcgg
     3601 ccagtacatg accttctcgg gaacgttgat gctcgtgctc ggcgccgtgg cggcgcggct
     3661 cgtgttgggt accaggggtc ggatgtggcc ggcgctcgtc atgcccgcgc tcgtcgtggc
     3721 gctctcgctg acgctgacgc gcagcgcgtg ggtgggtgcg tgcgtcagcg tggcgctcat
     3781 cttcatcctg aaggatctcc ggctgctggg cgtcctgccg gtcgtcggag cgctgctctt
     3841 tgccttcgct cccgacagcg tcacgaaccg gatgatgtcc accttcgatc tgcgcgaccc
     3901 gtcgaatcgc gatcgcctgg cgatgatgcg aagcggcagc gccatggtcc gcgaccaccc
     3961 gctgaccggt gtggggcccg aaatgatccc gcgggtgtac gcgcagtacc gggaccccga
     4021 cgcggtccag gccaccaacc gccacctcca caacgttccg atgcagatcg cggcggagag
     4081 ggggctgccg gcgctcgccg tctggatcgc gtttgtcgcc gtcctgacgt tcggcctgtt
     4141 ccgcctgttc cgcgcgggac gcgatcccgc cctggccgga gccgcgctcg cggcgatcgc
     4201 aggaatgctg agcgcggggt tcttcgagta caacttcggc gactccgagt tcctgatgct
     4261 gttcctcgtg atcgtgaccc tgccgtttgc cgcgtcccgc ccggacgatg ctgctgccaa
     4321 ccctcacgcg tgaccgcgcg cgcgagatca tgtctcgcgc cgtcggcgcc ggcgtgctcg
     4381 tcatcggcga tgcgatggtg gacaagttca tcgtgggacg ggtgagccgc atctcgccgg
     4441 aggcgcccgt gcccgtggtc gccttcgacc acgacctcca tcgaatgggg ggcgccgcca
     4501 atgtggcaag caatatcgtg gcgctcgggg gacaggcgac gatcgtggcc gtgaccggcc
     4561 gcgacgaggc ggccgcgacg ctgaccgagg cctgccggga agcggggatc gcgccctcgc
     4621 tcgtgagcga cgcgagccgc ggaacgacga ccaaggtccg ggtggtcacg gaccggaatc
     4681 agcaggtcgc gcggatcgac tacgaagtgg atgacgagat cgcgggagaa atcgagagcc
     4741 gggtcatcgc ggaaatcggg aagcacgcgc cgagggcctc ggcgatcgtc ctctcggact
     4801 acctgaaggg ctgcgtcacg cggcaggtgg tgcagacggc gcttgctgcg gcggcccgcc
     4861 acggggtgcc gctgctcgtc gacccgaaga ttccgcacct gccgtattac gcgggcgcat
     4921 cgatcgtgac tccgaaccac catgaggcgg aggccgcgac cggcgtgcgg gtgaggagcg
     4981 aagacgacgc gcgccgggcc gcgcggctct ttcgagagcg ggccggctgc cgcgacgtcg
     5041 tgatgacgcg aggcgatcag ggcatctggc tgctgagcga ccgcgtggag ggccacctcc
     5101 cggcggccgc acgcgaagtg gccgacgtca ccggggcggg cgacaccgtc gtggccacgc
     5161 tggcgctcgc gct
//
LOCUS       DBNGSH010000909         6684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3001419, whole genome shotgun sequence.
ACCESSION   DBNGSH010000909 DBNGSH010000000
VERSION     DBNGSH010000909.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3001419"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="AC16CY_20830"
     CDS             complement(<1..698)
                     /locus_tag="AC16CY_20830"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3356310.1"
                     /translation="MTSAVNVGRTRRVVTFGEIMLRLSPPGFERFLQSPAFSATFGGG
                     EANAAVSLAHFGLDSVYVTALPAHAIGDAAIRALRAEGVRTEHVVRGGSRVGIYFAET
                     GASQRASTVVYDRAHSSISEMTPDALSWDGILAGADWFHVSGITPALGASAAESTRRA
                     IGAARTAGAKVSFDLNYRRKLWTEADAQRVVGPLLRDVHLVIANEEDLQSMLGIHVAG
                     TDATAGHLDVDGFR"
     gene            complement(695..1348)
                     /locus_tag="AC16CY_20835"
     CDS             complement(695..1348)
                     /locus_tag="AC16CY_20835"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR3356311.1"
                     /translation="MTARQGVVSAIEEAGVVAVIRIREAGRLRAIVDALIDGGVRVLE
                     VTMTVPGAIDQIRDLSASLPAGLLLGAGTVLDEATAERVIDAGARFVVSPVFKPGLIE
                     VCHHRDVAALPGCFTPTEILTAWESGADLVKVFPATALGPTFFKDVKGPLPDVKLIPT
                     GGVTLDNAGDWIRAGAAAVGVGTALLDARAIAAGDYRVLTANAARIMAGVRAARGQQ"
     gene            complement(1345..2130)
                     /gene="kduD"
                     /locus_tag="AC16CY_20840"
     CDS             complement(1345..2130)
                     /gene="kduD"
                     /locus_tag="AC16CY_20840"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HIR3356312.1"
                     /translation="MTPRREVPVLDLFRLDGRTALVTGASRGLGAAMARALAEAGADV
                     ALHASRPLSPERTRELTSACGGRARVLTADLASRGEAERLADDAIAALGRIDILVNNA
                     GIIRRADADAYPDKDWDDVLMVDLSSVFRLCRSVGRTMLARGSGKIINIASMLTFQGG
                     MRVPAYAAAKGGVAQLTKALANEWAARGVNVNAIAPGYMRTDNTQALQDDRPRYEAIT
                     ARIPAGRWGEPDDLAGAVVFLASPASDYIHGYVLAVDGGWLAR"
     gene            complement(2319..3146)
                     /locus_tag="AC16CY_20845"
     CDS             complement(2319..3146)
                     /locus_tag="AC16CY_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR3356313.1"
                     /translation="MAVLPISDRTCIVRNTGATRGRTRSIEPGTAAVKHLRYGRIVLG
                     AADAPVEVPAGEVETALIALGGEAAVALAGVEYELRPYDSLYIPRGTAFQVRAGSGGC
                     DLAEIAAPVERQYPVQFVSFESVSRDPGLHFETGGDGARRTLNILVGKNVSAGRLMAG
                     VTFSQPGNWTSWPPHEHGVLAEEAYLFIDMPRPAFGVQLVYVDDTSPELATIVREGDV
                     VLMPQGYHPNVAAPGHPINFLWMMAAERELVDRQFGVVNVHPDFASGGSGLDRGRAR"
     gene            complement(3158..4033)
                     /gene="iolB"
                     /locus_tag="AC16CY_20850"
     CDS             complement(3158..4033)
                     /gene="iolB"
                     /locus_tag="AC16CY_20850"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR3356314.1"
                     /translation="MATWYTDVKARRLAIARFQTLSWLLKWIDMLKDTLFRQPRRTGL
                     HVLQRCGEAGARELTTRRLRLEAGATASLRLDEEETIAVVQDGSGMLAAGGSEWPVSR
                     KGVFAERATALYLPAGVEMQARSDSMLEAVLFSTRARPGGSPALIRPEQVAVAVRGRG
                     SYTREVHNIFVSDPHAQRLMVGETFNPPGNWSSYPPHKHDGRDGEPTLEEVYYYRVDP
                     PHGFGQQMLYTRDGESVTHTVLDGDAVLLPYGYHPVSAPPGYRLYYLWGLAGAERRLA
                     LHEDPAHQWIHSASL"
     gene            4067..5509
                     /locus_tag="AC16CY_20855"
     CDS             4067..5509
                     /locus_tag="AC16CY_20855"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR3356315.1"
                     /translation="MSRISRRDFMVAAAAAAVARPSILDGRGLPAVARTAKAGQRSAA
                     DPWVRARAIVESITVPRFPSRDFEITRYGAVGDGVASCTSAIGKAVAACRAAGGGRVV
                     VPRGRYLTGAVCLESGVNLHLAEGATLAFSAAPADYPLVFTRWEGVELMNYSPFIYAF
                     EAENIAITGTGTLDGQAGPDRWWNWRGTGAARGARQTSGRTRLFDMQARGVPVAERIF
                     GDGHFLRPNFIQPYRSRNVLIDGVTIVNSPMWEIHPVLCRSVTVRNVTITSHGPNNDG
                     CNPESCRDVLIERCSFDTGDDCIALKSGRNDDGRRLNVPVENVVIRDCSMKDGHGGVV
                     IGSEISGGARNVFAERCRMDSPRLDRALRMKTNSVRGGVIEGVYMRDVTVGQVAEAVV
                     TIDFFYEEGDSGKYPPTVRDVEVRNVTSRKSQYALLLRGYGHAPITGVRIVDCRFDGV
                     EKADVLEAVRDLELANVSINGKALTSRITR"
     gene            complement(5487..>6684)
                     /gene="pelA"
                     /locus_tag="AC16CY_20860"
     CDS             complement(5487..>6684)
                     /gene="pelA"
                     /locus_tag="AC16CY_20860"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HIR3356316.1"
                     /translation="GGRRHHAGLAFVHRTLPLRAMTVARYWYSHFAMRTQAGSFRRGP
                     RALARVVIGACAAALAATSPAAQVPWSRVLDQQAEWYGSAEARSIADSVLLHQRANGG
                     WPKDIDMSTPPSGAAARPSEPDSTIDNGATVTQIRLLDRVWLSTTNARYRDAAARGLD
                     YLFLSQYANGGWPQIYPLRKDYTRRITFNDDAMAGVLLLLDDLVAGRALASAGDVARA
                     RAVKAIDTATSVILRSQVRVNGRLTAWCAQHDEVTLEPAPARSYEHVSLSGRETVDIV
                     RFLMRRPPTPEVVAAVEAAVEWLRSVPIRGRRLDRRADPAGRRAPDLELVVDPAAPAL
                     WARFYQIGTNRPIYSGRDGVVRYDISEIEAERRNGYAWLGPWPAALVDAEYPAWRARV
                     SGLSVK"
BASE COUNT         1035 a         2309 c         2287 g         1053 t
ORIGIN      
        1 tcgcggaagc cgtcgacatc cagatgcccg gccgtcgcat cggttcccgc gacatggatg
       61 ccgagcatcg actgcagatc ttcttcgttg gcgatgacga ggtgcacgtc acggaggaga
      121 gggcccacga cgcgctgcgc gtcggcctcg gtccagagct tgcggcggta gttcaggtcg
      181 aagctgacct tcgcgcccgc cgtccgcgcc gcgccaatgg cgcgccgggt ggactcggcc
      241 gcgctcgcgc cgagggcggg cgtgattccg ctgacgtgaa accagtcggc gccggccagg
      301 atgccgtccc acgagagggc gtccggcgtc atctcggaaa tcgaggagtg cgcgcggtcg
      361 tagacgacag tcgaggcgcg ctgactggcg cccgtttccg cgaagtagat gccgacgcgg
      421 ctgccgccgc gcacgacgtg ctcggtgcgc acaccttcgg cgcgtagcgc ccggatcgcc
      481 gcgtcgccga tcgcgtgcgc gggcaacgcg gtgacgtaga cgctgtccag gccgaagtgc
      541 gccaggctca ccgctgcgtt cgcctcgcct ccgccgaagg tcgccgagaa cgccggggac
      601 tgcagaaagc gctcgaaccc cggcgggctc agccgcaaca tgatctcgcc gaaggtgacc
      661 acccgtctgg tccggccaac gttcacggcg cttgtcactg ctggcctctc gccgcccgca
      721 cgccggccat gatacgcgcg gcgttggccg tcagcacgcg gtagtcgccc gccgcaatcg
      781 cccgggcgtc gagcagggcc gtgccgacgc caaccgccgc ggcgcccgcg cgaatccagt
      841 cgcccgcgtt gtcgagcgtc acgccgccgg tcggaatcag cttcacatcc gggagcggtc
      901 ccttcacgtc cttgaagaag gtcggaccga gcgcggtggc gggaaacacc ttgaccagat
      961 ccgcgcccga ttcccaggca gtcaagatct ccgttggcgt gaagcatccc ggcagtgccg
     1021 cgacatcacg gtggtgacat acctcgatga ggcctggctt gaacacgggg ctgactacga
     1081 accgcgcacc cgcatcgatc acgcgctccg ccgtggcttc gtcgagcacg gtgccggcgc
     1141 ccagaaggag tcctgccggc agcgaagccg atagatcccg gatctgatcg atcgctcctg
     1201 gcaccgtcat cgtgacttcg aggacgcgca cgcccccatc gatcaaggca tcgacgatgg
     1261 ctctcaatcg gccggcttcg cggatccgga tgacggcgac gacgccggct tcttcgatcg
     1321 cggagacgac gccctgtcgg gccgtcatcg tgccagccac ccgccgtcca cggcgagcac
     1381 gtatccatgg atgtaatccg acgcgggcga ggccaggaag accaccgcgc cggccagatc
     1441 gtccggctcc ccccagcggc ccgccggaat gcgcgcggtg atcgcctcgt atcggggccg
     1501 atcgtcctgt aacgcctgcg tgttgtcggt gcgcatgtag ccgggcgcga tcgcgttgac
     1561 gttcacgccc ctggccgccc actcgttggc aagcgccttg gtgagctgag cgacgccgcc
     1621 cttcgcggcg gcgtacgccg ggacgcgcat ccctccctga aaggtcagca tcgaggcgat
     1681 gttgatgatc ttccccgagc ccctcgccag catcgttcgc ccgacgctgc ggcacagcct
     1741 gaacacgctc gacaaatcga ccatcagcac gtcgtcccag tctttgtcgg ggtatgcgtc
     1801 ggcgtccgcc cgccggatga tgcccgcgtt gttgacgagg atgtcgatcc tcccgagggc
     1861 ggcgatggcg tcgtcggcaa gccgctcggc ctcgccgcga ctggcgagat cggccgtcaa
     1921 cacgcgcgcg cgccctccgc acgcgctcgt gagctcgcgg gttcgttccg gggagagcgg
     1981 acggctggcg tgtaacgcga catccgcccc ggcctcggcg agcgcccgcg ccatggcggc
     2041 gccgaggccg cgtgaggccc ccgtgacgag ggccgttcgt ccatcgagcc tgaacaggtc
     2101 gaggaccggc acttctctgc gtggcgtcat ctgtcgagaa cgagatccgt tgttcacact
     2161 tctcggttct gaccttctca gttcgtgttc aatttcggag ttcgtgttcg cagttctggg
     2221 ttcgggttcg aggttccggg ttcgagcccg agcggagcgt acgcagtgtc cgggaaatgt
     2281 gaacgacaga tctcatgctg agttcaaggc ctccgcgatc agcgtgcgcg tccgcggtcc
     2341 aggcccgagc cgccgctcgc gaagtcgggg tggacgttca cgacgccgaa ctgacgatcc
     2401 acgagctcgc gctcggcggc catcatccag agaaagttga tcggatgccc gggcgcggcg
     2461 acgttgggat ggtagccctg cggcatgagc acgacgtcgc cctcccggac gatcgtcgcc
     2521 agctcggggc tggtgtcgtc cacgtagacc agctgcacgc cgaacgccgg ccgcggcatg
     2581 tcgatgaaga gatacgcctc ctcggccagc acgccgtgct cgtgcggcgg ccatgacgtc
     2641 cagttccccg gctggctgaa ggtcacgccg gccatcaggc ggccggccga gacgttcttg
     2701 ccaaccaaga tgttcagcgt ccgacgcgcc ccgtcgccgc cggtttcgaa atgcaggccc
     2761 gggtcgcgcg agacgctttc gaaggagacg aactgcacgg ggtactgccg ttcgacgggt
     2821 gcggcaatct cggcgagatc gcatccgccg gacccggcgc gcacctgaaa cgcggtcccg
     2881 cgcgggatgt acagcgaatc gtaggggcgc agctcgtact cgaccccggc gagcgccacc
     2941 gcggcctcgc ctccgagcgc aatcagggcg gtttcgacct cgccggccgg gacctcgacg
     3001 ggcgcgtcgg cggcgcccag cacgatgcgc ccgtatcgca ggtgcttgac cgcggcagtc
     3061 cccggctcga tcgatcgtgt acgcccccgg gtggcgccgg tgttccgcac gatgcaggtt
     3121 cggtcggaga tcggaagaac agccatcggc ctgcgcgtca gagactggcg gagtgaatcc
     3181 actgatgggc ggggtcctcg tgcagggcga gacgccgctc ggcccctgcc aggccccaca
     3241 ggtaatagag ccggtatccg ggcggcgccg agaccgggtg gtagccatac ggcagcagca
     3301 ccgcgtcgcc gtcgaggacg gtgtgcgtga cgctttcgcc gtccctggtg tagagcatct
     3361 gctggccgaa gccgtgcggc ggatcgacgc gatagtagta cacctcttcg agcgtgggct
     3421 caccgtcgcg gccgtcatgc ttgtgcgggg gatagctgct ccagttccct ggagggttga
     3481 acgtctcgcc gaccatcagg cgttgcgcgt gcggatcgct gacgaagatg ttgtgcacct
     3541 cgcgcgtgta gcttccccgg ccgcgcaccg ccaccgccac ctgctcgggc cggatgagcg
     3601 cgggcgagcc gcccggcctg gctcgggtcg agaagagcac cgcctcgagc atcgaatccg
     3661 aacgcgcctg catctcgacg ccggccggca ggtacaacgc cgtggcgcgc tcggcaaaca
     3721 cgcccttccg gctgaccggc cactcgctgc cccccgctgc cagcatcccg ctgccgtcct
     3781 ggacgaccgc gatcgtctct tcctcgtcca gccgcagcga ggccgttgcg ccggcctcca
     3841 gcctcagccg ccgcgtcgtc aactcgcggg cccccgcctc gccgcaccgc tgcaggacgt
     3901 gcagccccgt gcgcctcggc tgtctgaaca gcgtgtcctt caacatgtca atccacttga
     3961 ggagccagct tagcgtttga aaccgcgcga tggcaagcct gcgcgccttg acatcggtgt
     4021 accaggtcgc caagatgtgt cacctcctgt aggtggtctg tccactatga gtcgcatctc
     4081 gaggcgggac ttcatggtcg cggctgcggc ggcggccgtc gcgcggccgt cgatcctgga
     4141 cgggcgcggt ctgcccgctg tagcccgaac ggcgaaggca ggccagcgca gcgccgccga
     4201 tccgtgggtg cgcgcgcgcg ccatcgtcga gagcatcacg gttccgcggt ttccctcccg
     4261 agacttcgag atcacccggt acggcgcggt tggagacggg gtcgcgtcgt gcacgagcgc
     4321 catcggcaag gccgtcgccg cctgccgagc cgctggcggc gggcgggtgg tggttccgag
     4381 gggacggtac ctcactggcg cggtgtgtct cgagagcggc gtgaaccttc acctggcgga
     4441 gggcgccacg ctcgcgttca gcgccgctcc ggccgactac ccgctcgtgt tcacccggtg
     4501 ggagggcgtc gagttgatga actactcccc gttcatctac gccttcgaag ccgagaacat
     4561 cgcgatcacc ggcacaggca cgctggacgg ccaggccggg cctgaccggt ggtggaactg
     4621 gcgcggcacc ggcgcggccc gcggcgcgcg ccagacctcg ggccggacgc gtctgttcga
     4681 catgcaggcc cgcggcgttc cggtcgccga gcggatcttc ggcgacggcc acttcctcag
     4741 gccgaatttc atccaaccct accggagccg caacgtgctg atcgacggag tcacgatcgt
     4801 caactcgccg atgtgggaaa ttcaccccgt gctctgccgg agcgtcacgg tccgcaacgt
     4861 caccatcacg agccacggcc cgaacaacga cggctgcaat cccgagtcgt gccgcgacgt
     4921 gctcatcgag cgctgctcgt tcgacaccgg cgacgactgc atcgcgctca aatccgggcg
     4981 caacgacgac ggccggcggc tgaacgtgcc ggttgaaaac gtcgtgattc gcgactgctc
     5041 gatgaaggac ggccatggcg gcgtcgtcat cggcagcgag atctcgggcg gcgcccgaaa
     5101 cgtgttcgcc gagcggtgcc gcatggacag cccccgcctc gatcgagcgt tgcgcatgaa
     5161 gacgaactcg gtgcgagggg gcgtgatcga gggcgtctac atgcgcgacg tcacggtggg
     5221 tcaggtggcg gaggcggtcg tcacgatcga cttcttctac gaggaagggg attcggggaa
     5281 gtaccctccg acggtccgcg acgtcgaggt tcgcaacgtg accagccgga aaagccaata
     5341 tgcgctgctg ctgcgcggct atggtcacgc tccgattacg ggcgtgcgca tcgtggattg
     5401 ccgcttcgac ggcgtggaaa aggcggatgt gctcgaagcg gttcgcgatc tcgagctggc
     5461 caacgtgtcc atcaacggca aggcgctcac ttcacggata acccgctgac gcgggcgcgc
     5521 cacgccgggt attccgcgtc aacgagcgcg gccggccacg ggcccagcca ggcatatccg
     5581 ttccggcgct cggcctcgat ctccgagatg tcgtatcgca ccacgccgtc gcggcccgag
     5641 tagatgggcc tgttcgttcc gatctgatag aagcgcgccc agagagcggg cgctgccgga
     5701 tcgacgacga gctcgagatc gggcgcgcgc cgtccagcgg ggtcggcgcg gcggtcgagc
     5761 cgccgaccgc gaatcgggac cgatcgaagc cactcgacgg cggcctccac cgccgcaacg
     5821 acctccggag tcggcggacg gcgcatcagg aagcgaacga tatcgacggt ctcgcggccg
     5881 ctgagcgaca cgtgttcgta cgatcgggcc ggagcgggtt cgagcgtcac ttcgtcgtgc
     5941 tgcgcgcacc aggccgtgag gcggccgttg acgcggacct gcgatcggag aatcaccgac
     6001 gtggcggtgt cgatggcctt gaccgcgcga gcccgtgcga cgtcaccggc ggatgcgagc
     6061 gcccgccccg ccacgagatc gtccagcagc aggaggaccc cagccatcgc gtcgtcgttg
     6121 aacgtgatgc ggcgggtgta gtccttccgc agggggtaga tctgcggcca tccgccgttg
     6181 gcatattgag agaggaacag atagtcgagc cccctcgccg cggcgtcccg gtacctcgcg
     6241 ttcgtcgtgg acagccacac tcgatcgagc aggcggatct gggtgaccgt ggcgccgttg
     6301 tcgatcgtcg agtcgggctc ggagggtcgc gctgctgcgc ccgagggcgg agtgctcatg
     6361 tcgatgtctt tcggccaccc gccgttcgcc cgctgatgga gcagcacgct gtcggcgatc
     6421 gagcgcgcct ccgcggagcc gtaccactcg gcctgttgat cgagcacgcg gctccacggc
     6481 acctgagcgg ccggcgacgt cgccgccagc gccgccgcgc acgcgccgat cactacgcgt
     6541 gcgagcgcgc gcggcccgcg gcggaaagac ccggcttgcg ttctcatcgc aaagtggcta
     6601 taccaatacc gcgcgaccgt catggcgcgc aaggggagcg ttcgatgcac aaaggccaac
     6661 ccggcgtggt gacgtctgcc gccg
//
LOCUS       DBNGSH010000910         3736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4191838, whole genome shotgun sequence.
ACCESSION   DBNGSH010000910 DBNGSH010000000
VERSION     DBNGSH010000910.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4191838"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1805)
                     /locus_tag="AC16CY_20865"
     CDS             complement(<1..1805)
                     /locus_tag="AC16CY_20865"
                     /inference="COORDINATES: protein motif:HMM:NF019259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3356317.1"
                     /translation="MNDRISFYGRIVPVAAASWLLAMAAVWGGTEPVGAEQQQPPSVA
                     APTDTPAFLKQYCIGCHNTRANIGGLALDALDVTRVGPAAETWEKVVKKVRTGMMPPS
                     GARRPERAALDAFASDLEARLDKAADVNAALATPALHRLNRTEYANAIRDLLALDVDV
                     NALLPADGSSQGFDNLAEALAVSPSLVQGYVSAAMKISRLAVGDRTLAPSQVTYGPAA
                     GLAQDRHIDGLPLGTRGGVLIRHTFPLDAEYEFSLGGRGGGPGGGIDITLDGEQVRLT
                     GRGVRMKVPAGPHTIGVAVVDRQRGAGIDDIYSDFRADAAFATGGGVPNLVITGPHNA
                     TGPGDTPSRRLIFTCRPASAKEEPAPASATATARSRRSSQTIEASEGGCARTILSALA
                     RRAYRGPVSAPEIDTLMGFYQQGRKTGDFDSGIQQALARVLVAPRFVYRAEEEPATVR
                     QGQAYRLSDVDLASRLSFFIWSSIPDQELLDVAIAGRLREPNVLKQQLARMLADQKAD
                     ALIDNFAGQWLYLRELENIQTEAKSFDANLRRSFRRETELLFGAIVREDRSLIDLLDS
                     DYTFVDERLARHYGIPNVYGSYFRRVTLGPDSPRRG"
     gene            complement(1802..3355)
                     /locus_tag="AC16CY_20870"
     CDS             complement(1802..3355)
                     /locus_tag="AC16CY_20870"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3356318.1"
                     /translation="MKRTVGEIGWACLAAVLLVMLAPAPGTAAGDTRLADAAMRRDLA
                     AVRTLIGQKVDVNAPGVDGTPALHWIVRVDDVATARLLLGAGAQPSLPNRYDLRPLAI
                     AASNGSAAMIGVLLDAGADPNAPDPAGDTPLMSVARVGSVDAAVLLLDRGATVDATDA
                     TYQQTALMVAVRANRPEIVKLLVARGAGVNLKTRVGRTPQWVLPNSVPGFGHGIGIVR
                     GGLPPRGSRAPIPGGMTPLLYAARDGRIEIARVLLDAGADVNLADANGITPLIGAITN
                     NHPEAARFLIDRGADIRAADWYGRTPLWAAVETRNMDVDNASFVNSIDRAPYLPLIEL
                     LLQRGADPNVRMKEVPPIRRDFLRITGSLAWVDFTGQTPFLTASLAGDLAVMKMLLAH
                     GADPNIPTFGGTTALMAAAGVNWVVDQTYDEGQAALLEAVKLCVELGMDVNAVNSMGL
                     TAAHGAANRGSDEILRFLVQQGAKLDVKDAEGRTPLIWAEGVFLATHPSRPKPSSIAL
                     IKQLTPSAP"
     gene            complement(3428..>3736)
                     /locus_tag="AC16CY_20875"
     CDS             complement(3428..>3736)
                     /locus_tag="AC16CY_20875"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3356319.1"
                     /translation="ADDTVANGRLFVAHGSDGMRVDQKGNLYTTSGGGPGVIQITSPE
                     GKPLGRLHLPQPASEPRVRICATNVAFGDADNRGLYITACTHLFKIRLNQPGVRPGRR
                     "
BASE COUNT          548 a         1282 c         1289 g          617 t
ORIGIN      
        1 cctctgcgcg ggctgtccgg cccgagcgtc acccgccgga aataactgcc gtagacgttg
       61 gggatgccgt aatgacgggc gagccgttcg tccacgaacg tgtagtcgga gtcgaggaga
      121 tcgatgaggc tgcgatcctc gcggacgatg gcgccgaaga gcagttccgt ttcgcgtcga
      181 aacgatctcc gcaggtttgc gtcgaagctc ttggcttccg tctggatgtt ctcgagctcc
      241 cgcagataga gccactggcc cgcgaagttg tcgatcagtg catcagcctt ctggtccgcg
      301 agcatccgcg caagctgctg cttgaggacg ttcggctcgc gcagccgccc cgcgatggcg
      361 acatcgagca gttcctgatc cgggatgctg ctccagatga aaaacgacag gcgagacgcc
      421 agatccacgt cgctgaggcg atatgcctgt ccctgacgta cggttgcggg ctcctcctcg
      481 gcgcggtaca cgaatcgcgg cgccaccagg acgcgggcca gcgcctgctg gatgcccgag
      541 tcgaagtcgc ccgtcttccg cccctgctgg tagaacccca tcagcgtgtc gatttcaggc
      601 gcgctgaccg gcccgcggta cgcgcgcctg gccagtgccg acagaatcgt gcgcgcgcat
      661 ccgccttcgc tcgcctcgat cgtctgcgag ctacggcgag acctcgccgt agccgtggcg
      721 gaggcggggg ccggttcctc tttcgcggag gcgggccggc aggtgaagat cagccttcga
      781 ctcggcgtgt cgcccgggcc ggtcgcgttg tgcggtccgg tgatgaccag gttcggcacg
      841 cctcccccgg tcgcgaacgc cgcatcggcg cggaagtccg agtagatgtc gtcgatcccc
      901 gctcctcgct gccgatcgac gacagcgacg ccgatcgtgt gcgggccggc gggcactttc
      961 atgcggacgc ctcgaccggt gagcctgacc tgctcgccgt cgagcgtgat gtcgatgccg
     1021 ccgcccggtc ctccgccgcg cccgcccagg gagaattcgt actcggcgtc gagcggaaac
     1081 gtatgtcgaa tcagtacgcc tccgcgtgtg ccgaggggga gcccgtcgat gtggcgatcc
     1141 tgcgcgagcc ccgcggccgg tccgtaggtc acctgcgaag gcgcgagcgt ccgatcgccc
     1201 acggccagac ggctgatctt catggcggcc gagacatagc cctggacgag cgacggcgag
     1261 acggccagcg cctcggccag gttgtcgaac ccctggctcg acccgtcggc gggcagcagc
     1321 gcgttcacgt ccacgtcgag cgccagcaga tcccggatgg cgttggcgta ttcggtgcga
     1381 ttcagccggt gcagggcggg cgtcgccagc gccgcgttga catcggccgc cttgtcgagc
     1441 cgcgcttcca gatccgacgc gaacgcatcg agcgccgcgc gctcggggcg gcgggcaccg
     1501 cttggaggca tcatgcccgt gcgtactttc ttgacgacct tctcccacgt ttccgcggca
     1561 ggtcccacgc gcgtcacgtc gagcgcatcg agcgcgagtc cgccgatgtt ggcgcgcgtg
     1621 ttgtgacagc cgatgcagta ctgcttcaga aacgcgggcg tgtcggtggg tgcggcgacg
     1681 ctgggcggct gctgctgctc ggcgcccacg ggctccgtgc cgccccacac ggccgccatc
     1741 gcgagcagcc acgacgcggc cgccaccggc acgatccgcc cgtaaaaact gattcggtcg
     1801 ttcatggcgc cgacggcgtc agttgcttga tcaaagcgat cgagctgggc ttgggccggc
     1861 tcggatgcgt cgccaggaac acgccttcgg cccagataag cggcgtgcgt ccttcggcgt
     1921 ccttgacatc gagctttgct ccctgctgca cgaggaagcg aaggatctcg tccgagccgc
     1981 ggttggcggc gccgtgggcc gcggtcaacc ccatcgagtt caccgcgttg acgtccattc
     2041 cgagctcgac gcacagcttg acggcctcga gcagcgccgc ctggccctcg tcgtaggtct
     2101 gatcgacgac ccagttgacg cccgcggccg ccatcagcgc cgtcgtcccg ccgaaggtcg
     2161 ggatgttcgg gtcggcgccg tgtgccagca gcatcttcat caccgcaagg tctccggcga
     2221 gcgacgcggt caggaacggc gtctgcccgg tgaagtccac ccacgcgagc gaccccgtga
     2281 tgcggaggaa gtcgcgcctg atcggcggca cctccttcat gcggacgttc gggtcggcgc
     2341 cgcgctggag cagcagctcg atcagcggca ggtacggcgc gcggtcgatg ctgttgacga
     2401 acgaggcgtt gtccacgtcc atgttgcgcg tctcgacggc agcccacaac ggcgtgcggc
     2461 cgtaccagtc ggcggccctg atgtcggccc cgcggtcgat cagaaagcgg gcggcctcag
     2521 gatgattgtt cgtgatcgcg ccgatgagcg gcgtgatgcc gttggcgtcg gccaggttca
     2581 cgtccgcacc ggcgtcgagc aggacgcggg caatctcgat tcggccgtcc cgcgccgcat
     2641 agagcagggg agtcataccg ccgggaatcg gcgcgcgcga cccgcgcggc ggcagtccgc
     2701 cgcggacgat gccgatgccg tgtccgaagc cgggaaccga gttgggcagc acccattgag
     2761 gcgtccgtcc aactctcgtc ttcaggttca caccggcgcc gcgggcgacc agcagcttca
     2821 cgatctcggg acgattcgcc ctgacggcca ccatgagcgc cgtctgctgg taggtcgcgt
     2881 cggtcgcgtc gaccgtcgcg ccgcgatcga ggagcagcac cgccgcgtcg acgctgccga
     2941 cgcgcgccac gctcatcagc ggcgtatcgc ccgccggatc gggggcgttc ggatccgcac
     3001 cggcgtcgag caggacgcca atcatggccg cgctgccgtt gctggcggca atggccagcg
     3061 gtctcaggtc gtatcgattc ggaagcgacg gctgcgcgcc cgcgccaagc agcaggcgtg
     3121 ccgtcgcgac atcgtccact cggacgatcc agtggagtgc cggcgtgccg tccacgccgg
     3181 gggcgttgac gtcgaccttc tggccgatca gcgtgcggac ggccgccaga tcccgccgca
     3241 tggcggcatc cgccagccga gtgtctccgg ccgcggtgcc tggggctggc gccagcatga
     3301 cgagcagcac ggcggccaga cacgcccacc cgatttcgcc gacggtcctt ttcatgtcct
     3361 cgatcctctt ctattagacg acgggtcgga cgcaatgaaa agggggcgac ggagccggga
     3421 accgggccta ccgccgtccc ggccgcacgc cgggctggtt caatcgaatc ttgaagaggt
     3481 gggtgcacgc cgtgatgtag agcccgcggt tgtcggcgtc cccgaaggcc acgttggtgg
     3541 cgcagattct cacgcgcggt tcgctcgccg gctgcggcag gtgcagcctc ccgagcggct
     3601 tgccttccgg cgacgtgatt tggatcacgc caggtccgcc gccgctcgtc gtgtagaggt
     3661 tgcccttctg atcgacgcgc atgccatcgg acccgtgcgc gacgaacagc cggccgttgg
     3721 cgaccgtgtc gtcggc
//
LOCUS       DBNGSH010000911         1901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1644586, whole genome shotgun sequence.
ACCESSION   DBNGSH010000911 DBNGSH010000000
VERSION     DBNGSH010000911.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1644586"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC16CY_20880"
     CDS             complement(<1..815)
                     /locus_tag="AC16CY_20880"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3356320.1"
                     /translation="MIGRRLAHYEITAHLGSGGMGDVYQATDSKLDRTVALKFLPQAF
                     AGDPDRSARFAREAKALAALNHPNIAAIHGLDDGEGGERFLVMEFVPGSTLADRIGRT
                     PLPLDEALGIARQIAEALEAAHDKGVVHRDLKPANVKITPEGQVKVLDFGLAKLAGGD
                     AEETGRALSNSPTLSAAVTRAGVVLGTAAYMSPEQAKGRLVDRRADLFAFGCVLYEML
                     AGRPAFEGESAADILSCVLQREPDWTRLPSSVPPAVRQLLRLCLEKDPRKRRQS"
     gene            complement(846..>1901)
                     /locus_tag="AC16CY_20885"
     CDS             complement(846..>1901)
                     /locus_tag="AC16CY_20885"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356321.1"
                     /translation="FASGADFQKILRRPNLTVDDAKAIERLAPSVGIVDIWMGQGMDQ
                     TAERVSYEGQRTPMLGVLGATEQFAAVNFVKLSFGRFFTESEVLRRRRVVVLADGPYQ
                     ALFAPSGLDPIGKKVRIGAIEYTVVGVFEKRPSPFPQADNFVVIPQTTHQVIFNTNAR
                     RMFGGGGSATITIVPVETATRDQAVAEIEEIMRIRHRLTLDEPNDFDIGGQDAILRIW
                     DQVSQAVFLALVVISSVALMVGGIGVMAIMMISVTERTREIGVRKALGARRQEILFQF
                     LAEAAALTSVGGILGVLIGAGIGAAISLATGFPVSLPWWSFAIGLGFSASVGIFFGMF
                     PAVRASRLDPIEALRHE"
BASE COUNT          324 a          650 c          621 g          306 t
ORIGIN      
        1 gactgacgcc gcttccgtgg atctttttcc aggcacagac gaagaagctg ccggaccgct
       61 ggcggcacgc tggacgggag gcgcgtccag tccggttcgc gctgcagcac gcacgacagg
      121 atgtcggcgg cgctctcgcc ttcgaacgcc ggccgcccgg cgagcatttc gtagagcacg
      181 caaccgaacg cgaacagatc cgcgcgccga tcgaccaatc gtccctttgc ctgctcggga
      241 gacatgtacg ccgccgtacc caggacgacg cccgcgcgtg tcaccgccgc gctcagcgtc
      301 ggcgagttcg agagcgctcg tcccgtctcc tccgcgtccc cacccgcgag cttggccagg
      361 ccgaagtcga ggaccttgac ctggccctcg ggcgtgatct tcacgttcgc cggcttgaga
      421 tcgcggtgca cgacgccctt gtcgtgcgct gcctcgagtg cctcggcgat ctgcctggcg
      481 atgccgagcg cctcgtcgag cggcagcggc gtgcgtccaa tccgatccgc cagcgtcgag
      541 cccggtacga actccatgac caggaagcgt tcgccgccct ccccgtcgtc cagcccgtgg
      601 atcgcggcga tattgggatg gttgagcgcg gcgagggcct tcgcttcgcg cgcgaaccga
      661 gccgagcgat cgggatcgcc ggcgaaggcc tgggggagga acttgagcgc gaccgtgcga
      721 tcgagcttcg agtcggtggc ctggtagacg tcgcccatgc cgcccgatcc gagatgggct
      781 gtgatctcgt agtgtgcgag ccgccggcca atcatgtcag cagacggccg tatcgtagat
      841 caaagctatt cgtgtctgag cgcctcgatc ggatcgaggc gcgacgcgcg gactgccggg
      901 aacatgccga agaagatccc gaccgacgcc gagaagccca gcccgatcgc gaacgaccac
      961 caggggagcg agacggggaa gccggtcgcc aggctgatgg cggcaccgat gcctgcgccg
     1021 atcaggaccc cgaggatccc tccgaccgac gtcaatgccg cggcctccgc caggaactgg
     1081 aagaggatct cctgcctgcg cgcgccgagc gccttccgga cgccgatttc tctcgtccgc
     1141 tcggtgaccg agatcatcat gatggccatg acgccgatgc cgcccaccat gagcgccacc
     1201 gacgaaatca cgacgagcgc caggaacacg gcctgactga cctggtccca gattcgcaga
     1261 atggcgtcct gcccgccgat gtcgaaatcg ttgggctcgt ccagcgtcag cctgtggcga
     1321 atccgcatga tctcctcgat ctcggcgacc gcctggtcgc gcgtggccgt ctcgaccggg
     1381 acgatggtga tcgtggcgct gcctccgccg ccgaacatgc gccgcgcgtt cgtgttgaag
     1441 atgacttgat gcgtcgtctg cggaatgacg acgaagttgt ccgcctgtgg aaagggcgac
     1501 ggccgctttt cgaacacgcc gacgacggtg tactcgatgg cgccgatgcg gactttcttc
     1561 ccgatcgggt cgaggccgga cggggcgaag agcgcctgat acggaccgtc cgccagcacg
     1621 actacgcgac gccggcgcag cacctcggac tccgtgaaga accggccgaa cgacagcttc
     1681 acgaagttga ccgcggcgaa ctgctcggtg gcgccgagca ccccgagcat cggcgtgcgc
     1741 tgtccctcgt aggaaacgcg ctcggcggtc tgatccatcc cctggcccat ccagatatcc
     1801 acgatcccaa ccgatggtgc cagtctctcg atggccttgg cgtcgtccac ggtcaagttc
     1861 gggcggcgca ggatcttctg aaagtcggca cccgaggcga a
//
LOCUS       DBNGSH010000912         9332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_796543, whole genome shotgun sequence.
ACCESSION   DBNGSH010000912 DBNGSH010000000
VERSION     DBNGSH010000912.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_796543"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16CY_20890"
     CDS             complement(<1..224)
                     /locus_tag="AC16CY_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356322.1"
                     /translation="MYSTRAVIAASVLMTAACGSASAQNDATTVIPAALMASADAWNK
                     ADLPGHIGLYTDNATFMGANGPIQGRERVG"
     gene            complement(274..663)
                     /locus_tag="AC16CY_20895"
     CDS             complement(274..663)
                     /locus_tag="AC16CY_20895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3356323.1"
                     /translation="MVIVVDSSIWIQAHRRPNGKTATILTKLLDADQVALALPVRLEL
                     LAGVSSRDRAALARGLSALPLLRPSAETWALIERWVPDAADKGFTFGLADWLVTALAN
                     EIDALIWSLDDDFTHLETLKMARLYSA"
     gene            complement(647..862)
                     /locus_tag="AC16CY_20900"
     CDS             complement(647..862)
                     /locus_tag="AC16CY_20900"
                     /inference="COORDINATES: protein motif:HMM:NF021474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HIR3356324.1"
                     /translation="MRTTLILPEGLVDAARETLGFTSKTDTVVYALREVVRRGRRDEL
                     TNLLGRVRFEFDPAGIRKRDRRRGDRR"
     gene            complement(920..2371)
                     /locus_tag="AC16CY_20905"
     CDS             complement(920..2371)
                     /locus_tag="AC16CY_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007301395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR3356325.1"
                     /translation="MGKPTGFIEIHRKKHPTRPVAERIQDWREVYLPYPDGELAKQGA
                     RCMDCGIPFCHQGCPLGNLIPDWNDLVYRDRWHSAIDRLHATNNFPEFTGKLCPAPCE
                     GSCVLGINEDPVTIKSIEAAIIDRAWDEGWVTPRTPAQATFKKVAIVGSGPAGLAAAD
                     QLASAGHSVTVFEKADRIGGLLRYGIPEFKMEKRFLDRRLAVMEEEGVVFRTGVHVGV
                     DVPASRLVSDYDAVLLAGGAEKPRDLQVPGRDLAGIHFAMEYLTLQNRRCEGDDIPDD
                     EFITAKDKHVVIIGGGDTGADCLGTVHRQGARAVYQLELLDRPPDARAEDNPWPLWPQ
                     IFRVSSAHEEGGDRLYAVSTQRFSGDAAGGVRALHAVKVEVVKNAGPRLQLVPVPGSE
                     FQIPTELVLLAMGFVGPERGGLLDGLGVTLTDRGNVARDSRWMTNVPGVFTAGDMQRG
                     QSLIVWAIAEGRSAARAIDQYLMGESQLPAPLD"
     gene            complement(2364..6977)
                     /gene="gltB"
                     /locus_tag="AC16CY_20910"
     CDS             complement(2364..6977)
                     /gene="gltB"
                     /locus_tag="AC16CY_20910"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR3356326.1"
                     /translation="MRATGHSEAPPAQGLYDPQAEHDACGVGFVVHIKGRRSYGIVRK
                     ALEVLVNLEHRGACGCEENTGDGAGILIQIPDAFLRKVAGVLLPAEGEYGTGLVFLPH
                     EKAARTRIKNLVAAVAEEEGQQLLGWREVPTDNRLVGPSARATQPVFEQVFIAARGAA
                     SLDRLLFERKLYVIRKRIEHAVNSLKMSALSRRFFFIVSLSCNTLTYKGMLTARQLEP
                     MFPDLSDPDVATALALVHQRFSTNTFPSWPLAHPYRFIAHNGEINTLRGNINWMRARE
                     GLLQSDLFGDDLKKLLPVIREGGSDTATFDNVLEFLVRGGRSLPHAILMMIPEPWSGN
                     EEMDPDRKAFYEYHASLMEPWDGPASIAFTDGTMIGAVLDRNGLRPSRYYVTKDDLVI
                     MASEVGVLDVPPENILVKERLHPGRIFLVDTSQGRIVADDEIKRELAAQHPYRAWLDK
                     HLVDIDDLPPARAVEPDHETVLNRQLAFGYTQEDLRILIGPMAMAGEEPLGSMGTDTS
                     LAVLSNRPRVLFDYFKQLFAQVTNPPLDAIREELVTSMGSTIGPERNLLKPEPQSCEQ
                     ITIEYPIIHNEHVAKLRHLPPDSPFRSITLSMLFDPEKGGAGLEQAMDELCRQASEAV
                     AGGYNILILSDRGHGPRHAPIPSLMATAGVHHHLVREGTRTRCALVVECGEAREVHHV
                     SLLMGYGAGVVNPYLAFETIDDMIRHGLLTGTTHKEAVARYIKALNKGILKVMSKMGI
                     STLASYCGAQIFEAIGLDQAFIDKYFTHTTSRVGGVGIDVIAEEVRLRHERAYGRRPA
                     GVAELDAGGEYQWRRDGEFHLFNPDTVFKLQHATRSGQYRIFKDYTRAVNDQHERRAT
                     LRGLFRLKPAGPAVPIEEVEPVESIVRRFATGAMSYGSISQEAHETLAIAMNRLGGKS
                     NTGEGGESPERYIPLANGDSKRSAIKQVASARFGVTSEYLVNADELQIKMAQGAKPGE
                     GGQLPGHKVYPWIAKVRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNSNPRARISV
                     KLVAEGGVGTVAAGVAKAHSDVVLISGHDGGTGASPLTSLKHAGLPWELGLAETQQVL
                     VLNKLRDRIVVQVDGQMKTGRDVVIGALLGAEEYGFATAPLVVMGCIMMRVCHLNTCP
                     VGIATQDPELRKKFTGTPEMVQNFFRFIAEEVRELMAELGFRKMDEMIGRADCLDAER
                     AVHHWKAKGLELAPLLHQPDLPASVARRRVRAQDHGLEATLDNDLIRAAMPAIEHKTP
                     VELTAYIRNADRTVGTMLGSEITRRHGGRGLPDNTLRITFTGSAGQSFGAFVPAGMTL
                     RLVGDANDGFGKGLSGGRLIVHPPTLAAFTSQDNIIIGNVGFYGATSGEGYVRGLAGE
                     RFCVRNSGAMVVVEGVGDHGCEYMTGGRVVVLGRTGRNFAAGMSGGVAYVVDLDGRFE
                     RRCNLEMVDLEPLVDADDVDFIRAAIVKHVTYTGSRYAEGLLRDFETVRRRIVKVMPR
                     EYRRALAEQAAREEAERKSLIEQLVVTGVVAASASGAETTVHG"
     gene            7249..>9332
                     /locus_tag="AC16CY_20915"
     CDS             7249..>9332
                     /locus_tag="AC16CY_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3356327.1"
                     /translation="MHAPMKYVEKGLALTANGLWQVFSTANRIKQNPSFIPRWSDKPL
                     LKSYEKTKPQLGWPRETDSLCPNCVREARQEILDGKRDVSVLLNEKVGEIKATIVEKD
                     GKIVMVKDCPIHGHFEDVMAIDTAFFKHLEEVFPGRDIRAHNDETLHNHGSSTVLHGR
                     GSVLTVDLTNRCNMMCDPCFMDANQVGFVHELTWEDIKQILDNAITIKPRRQMSVQFS
                     GGEPTMSPYFLDAIRYSRKVGYNSVQAATNGIEFAKSPEFAKAAAEAGLRYAYLQFDG
                     IGNAANSHRRVGNLFDVKLRAIENLYQAGVDIVPVITIVNGVNNEQVGRVVEFALDNP
                     KRINFLSFQPVSFTGRDEEVTPERRAAQRYTLSHLAHDVKNQTGIGEPIRDWFPISFM
                     STFTDWADVVHGPDREWGQLTCGCHPNCGIGMAVMIDKETKEAVPVTAFLHADQLAKD
                     VAKVNDAARGRFLSVVGMALALMRNYDPFKAPTHFRIIDLMKKFDKTFGATGKDYGKV
                     TGDRTMADIEKRRQDRWNFLFIAGMWFQDLFNYDFRRTEMCIIPYGTQQGEISFCAYN
                     TGIGWRNILEKMHMTATLTKWYDEHGRHEIFAGNKHVPLASKEHSLQLNAEAVAAGAQ
                     TDLDKMGVAKNAREEKLAARKKAQMTPEEIRQHAEMERLYRQEVLKEQPTLQIQGLGG
                     SKKKQDVQPVTH"
BASE COUNT         1571 a         3180 c         3043 g         1538 t
ORIGIN      
        1 tcgcccacgc gctcgcggcc ctgaatcgga ccgttggcgc ccatgaaggt ggcgttgtcg
       61 gtgtagaggc cgatgtggcc cggcaggtca gccttgttcc acgcatccgc gctcgccatg
      121 agcgcggccg ggatgacggt ggtcgcatcg ttctgagccg acgcggatcc acacgccgcg
      181 gtcatgagca cggacgccgc gatcacagca cgtgttgagt acattggaat cttctcccga
      241 tgtcaggatc cggacccgca ctccgtctca tggtcaggct gagtagagcc gcgccatctt
      301 caacgtttca agatgggtga agtcgtcgtc cagcgaccag atcagtgcgt cgatctcgtt
      361 cgccagcgcc gtgacaagcc agtctgccag cccgaaggta aaccctttgt ccgccgcgtc
      421 tggcacccac cgctcgatga gtgcccaggt ttcggcggag ggtcgcagca gcggcagagc
      481 agagaggccg cgtgcaagcg cggcccggtc gcggctggag acacccgcca ggagttccag
      541 tcgaaccggc agcgccagcg cgacctggtc ggcgtccagc agtttcgtca ggatggtcgc
      601 cgtctttccg ttcggccggc gatgcgcctg gatccagatc gacgagtcaa cgacgatcac
      661 cacgccgccg atctcgcttg cggattcccg ccgggtcgaa ctcgaacctc acccgaccaa
      721 gcaggttcgt gagctcgtcg cgacgtcccc gcctcacgac ctcacgcagg gcgtagacca
      781 ccgtgtcggt cttcgacgtg aaaccgaggg tctcacgcgc cgcgtcgacg agcccttccg
      841 gaagaatcag cgtggtgcgc atatgatacg accataatca tatgctttaa agcatatccc
      901 cgacaagcat cgcccgggct cagtcgagag gcgcaggaag ctgcgactcg cccatcaggt
      961 actgatcgat ggcgcgcgcg gcgctgcgcc cctccgcgat tgcccacacg atgagtgact
     1021 ggccgcgctg catgtcgccg gcggtgaaca cgcctgggac gttcgtcatc caccgcgaat
     1081 cgcgcgccac gttgccccgg tcggtgagcg ttacgccgag accgtcgagc aacccgccac
     1141 gctccggccc gacgaacccc atcgccagca ggaccagctc ggtcgggatc tggaactcgc
     1201 tgcccggaac cgggaccagc tgcaagcggg ggcctgcgtt cttcacgacc tcgactttca
     1261 ccgcgtgcag cgctcgcacg cctccggcgg cgtcgcccga gaaccgctgc gtcgagacgg
     1321 cgtagagccg gtcgccccct tcttcgtgcg ccgacgacac gcggaagatc tgaggccaga
     1381 gcggccacgg gttgtcctcc gcccgcgcgt cgggcggcct gtccagcagc tcgagctgat
     1441 acaccgcccg cgcgccctga cggtgcaccg tgcccaggca gtccgcgccc gtgtcgccgc
     1501 caccgatgat gacgacgtgc ttgtctttcg ccgtgatgaa ctcgtcgtcc gggatgtcgt
     1561 cgccctcgca gcggcggttc tgcagcgtga ggtactccat cgcgaagtgg atcccggcga
     1621 ggtcgcgccc cggaacctgg agatcgcgcg gcttctcggc gcctcccgcc agcagcaccg
     1681 cgtcgtagtc ggacaccagc cggctggccg gtacgtccac gcccacgtgg acgccggtgc
     1741 ggaacacgac gccctcctcc tccatcaccg ccaggcggcg gtcgaggaag cgtttctcca
     1801 tcttgaactc ggggattccg taccgcagca gcccgccgat gcggtcggcc ttctcgaaca
     1861 cggtgaccga atggccggcg gacgcgagct ggtcggccgc cgccaacccc gcgggaccgg
     1921 agccgacgat cgccaccttc ttgaaggtcg cctgcgcggg cgttcgcggc gtcacccatc
     1981 cctcgtccca cgcgcgatcg atgatcgctg cctcgatcga cttgatcgtg acggggtctt
     2041 cgttgatgcc gagcacgcac gacccctcgc acggcgcggg acagagcttg ccggtgaact
     2101 ccgggaagtt gttcgtcgcg tgcagccggt cgatcgccga gtgccagcgg tcacgataga
     2161 cgagatcgtt ccagtccgga atcaggttgc cgagcgggca cccctgatgg cagaagggga
     2221 tgccgcagtc catgcaccgc gcgccctgct ttgcgagctc tccatccgga tacggcagat
     2281 agacctcgcg ccagtcctgg atgcgctccg cgaccggacg cgtcggatgc ttcttgcggt
     2341 ggatttcgat gaacccggtc ggcttaccca tggacggtcg tctccgcccc ggaggcggac
     2401 gccgccacca cgcccgtcac cacgagctgc tcgatgaggg acttgcgctc ggcttcttcg
     2461 cgcgcggcct gctccgccag ggcgcgccgg tactcgcgcg gcatcacctt gacgatgcgc
     2521 cggcgcacgg tctcgaaatc cctcagcagc ccttccgcgt accggctgcc ggtgtaggtg
     2581 acgtgcttga cgatggccgc ccggatgaag tccacgtcgt cggcgtcgac gagcggttcc
     2641 agatcgacca tctcgaggtt gcagcgccgc tcgaaccggc cgtcgaggtc cacgacgtac
     2701 gccacgccgc cgctcatgcc cgccgcgaag ttccgccccg tgcggcccag gacgacgacg
     2761 cggccgccgg tcatgtactc gcagccgtgg tcgccgacgc cttcgacgac gaccatcgcg
     2821 ccgctgttgc ggacgcagaa acgctcgccg gccaggccgc gcacgtagcc ttcgccgctc
     2881 gtcgcgccgt agaagccgac gttgccgatg atgatgttgt cctgcgacgt gaaggcggcg
     2941 agcgtcggcg gatgcacgat cagccggcca ccggacaggc ccttgccgaa cccgtcgttg
     3001 gcgtcgccga cgagccgcag cgtcatcccc gcgggcacga acgcgccgaa gctctggccc
     3061 gcggagcccg tgaaggtgat ccgcagcgtg ttgtcgggca ggccgcggcc gccatgccgc
     3121 ctggtgatct ccgagccgag catcgtgccg acggtgcgat cggcgttgcg gatgtaggcc
     3181 gtcagctcca cgggagtctt gtgctcgatc gccggcatcg cggccctgat caggtcgttg
     3241 tcgagcgtgg cctcgagccc gtgatcctgc gcgcggacgc ggcgcctcgc caccgacgca
     3301 ggcagatcgg gctggtgcag cagcggcgcc agctcgagcc ccttcgcctt ccagtgatgg
     3361 acggcgcgtt cggcgtcgag gcaatcggcc cgcccgatca tctcgtccat cttccggaag
     3421 ccgagctcgg ccatcagctc gcgcacttcc tcggcgatga accggaagaa gttctgcacc
     3481 atctccggcg tgccggtgaa tttcttgcgc agctccggat cctgcgtggc gatgccgacc
     3541 gggcacgtgt tcaggtgaca cacgcgcatc atgatgcagc ccatgacgac gagcggcgcc
     3601 gttgcgaagc cgtactcctc ggccccgagc agcgcgccga tcacgacgtc gcgccccgtc
     3661 ttcatctgac cgtccacctg cacgacgatc cggtcgcgca gcttgttgag gacgagcacc
     3721 tgctgcgtct cggcgagccc gagctcccac ggcagccccg cgtgcttgag gctcgtcagc
     3781 ggcgacgcgc ccgtgccacc gtcgtgaccc gagatgagga cgacgtcgga gtgcgccttg
     3841 gccacgcccg ccgcgacggt cccgaccccg ccttccgcga cgagcttcac cgagatccgc
     3901 gcgcgcggat tggagttctt caggtcgaag atcagctgtg ccagatcctc gatcgagtag
     3961 atgtcgtggt gcggcggcgg cgagatcagg ccgacgcccg gcgtggagtg ccgcaccttc
     4021 gcgatccagg gatagacctt gtgcccggga agctggccgc cctctcccgg cttggcgccc
     4081 tgcgccatct tgatctgcag ctcgtcggcg ttcaccaggt actcgctcgt cacgccgaac
     4141 ctggcggagg cgacctgctt gatcgcgctt cgcttcgagt cgccgttggc cagcgggatg
     4201 taccgttccg gcgactcccc gccttcaccg gtgttcgact tgccacccag cctgttcatg
     4261 gcgatcgcca gcgtctcgtg cgcttcctgg ctgatcgacc cgtacgacat cgcgccggtc
     4321 gcaaagcgcc gcacgatcga ctcgacgggc tcgacctcct cgatcggcac ggcgggcccg
     4381 gccggcttca ggcggaagag ccccctgagc gtggcgcggc gctcgtgctg atcgttcacg
     4441 gccctggtgt agtccttgaa gattcggtac tgcccgctcc gcgtggcgtg ctgaagcttg
     4501 aacaccgtgt cggggttgaa caggtggaat tccccgtcgc ggcgccactg gtactcgccg
     4561 ccggcgtcga gctcggccac gcctgccggc cggcgcccgt aggcgcgctc gtgccggagg
     4621 cgcacttcct cggcaatcac gtcgatgccg acgccgccga cgcgcgacgt ggtgtgcgtg
     4681 aagtacttgt cgatgaacgc ctggtcgagg ccgatggcct cgaagatttg cgcgccgcag
     4741 tagctggcga gcgtcgagat ccccatcttc gacatcacct tgaggatccc cttgttcagc
     4801 gccttgatgt agcgcgcgac cgcctccttg tgggtcgtgc ccgtgaggag gccgtgccgg
     4861 atcatgtcgt cgatcgtctc gaaggcgagg tacgggttca ccacgccggc cccgtagccc
     4921 atgaggagcg agacgtgatg gacctcgcgc gcctcgccgc actcgaccac gagcgcgcac
     4981 cgcgtgcgcg tcccctcgcg gaccaggtgg tgatgcacgc ccgccgtggc catcagactc
     5041 ggaatcgggg cgtgtcgcgg gccgtggccc cgatccgaga ggatcaggat gttgtagccg
     5101 ccggcgaccg cttcgctcgc ctgccggcac agctcgtcca tcgcctgctc gagacccgcg
     5161 ccccccttct cgggatcgaa cagcatcgag agcgtgatcg atcggaacgg cgagtcgggc
     5221 ggcaggtggc gcagcttcgc cacgtgctcg ttgtggatga tcgggtactc gatcgtgatc
     5281 tgctcgcacg actgcggctc gggcttcagc aggttccgct cgggaccgat cgtcgagccc
     5341 atcgacgtga cgagctcctc acggatcgca tcgagcggcg ggttggtgac ctgcgcgaac
     5401 agctgcttga agtagtcgaa gagcaccctc ggccggttcg acaggacggc gagcgacgtg
     5461 tcggtgccca tcgatcccag cggctcttcg ccggccatcg ccatggggcc aatcaggatc
     5521 cgcagatcct cctgcgtgta gccgaatgcg agctggcgat tgagcaccgt ctcgtgatcg
     5581 ggctcgaccg cgcgcgccgg cggcagatcg tcgatgtcta ccaggtgctt gtcgagccac
     5641 gcccggtacg ggtgctgcgc cgccagctcc ctcttgatct cgtcgtcggc gacgatgcgg
     5701 ccctgggacg tgtcgacgag gaagatgcgg cccgggtgca ggcgctcctt gaccaggatg
     5761 ttctcgggcg gcacgtcgag cacgccgact tccgaggcca tgatcaccag gtcgtccttg
     5821 gtgacgtaat agcgtgaggg ccgcagcccg ttgcgatcga gcacggcgcc gatcatcgtg
     5881 ccgtcggtga aggcaatcga cgccgggccg tcccacggct ccatcagcga ggcgtggtac
     5941 tcgtagaacg ccttccggtc cgggtccatc tcctcgttcc cgctccacgg ctccggaatc
     6001 atcatcagga tcgcgtgcgg gagcgatcgg ccgccgcgga cgaggaactc cagcacgttg
     6061 tcgaacgtgg cggtgtcgct gccgccttcg cggatgacgg gcaggagctt cttcaggtcg
     6121 tcgccgaaga gatcgctctg gagcaggccc tcgcgggcgc gcatccagtt gatgtttccg
     6181 cgcagcgtgt tgatctcccc gttgtgcgcg atgaaccggt acggatgggc cagcggccac
     6241 gacggaaacg tattggtgct gaaccgctgg tgcaccagcg cgagcgcggt ggccacgtcc
     6301 ggatcggaca ggtcggggaa catcggctcg agttgccgcg ccgtcagcat gcccttgtag
     6361 gtcagcgtgt tgcacgagag gctgacgatg aagaagaagc ggcgcgagag cgcgctcatc
     6421 ttgagcgagt taacggcatg ctcgatgcgc ttgcggatga cgtacagctt ccgctcgaag
     6481 aggaggcggt cgaggctggc ggcgccgcgc gcggcaatga agacctgctc gaacaccggc
     6541 tgcgtcgccc gcgcgctcgg gccaacaaga cggttgtcgg tcggcacctc ccgccacccg
     6601 aggagctgct gcccttcttc ttcggcgacc gctgcgacga ggttcttgat tcgagtgcgg
     6661 gccgccttct catgcggcag gaagacgagg ccggtgccgt actcgccttc cgccggcagc
     6721 aacacccccg cgacctttcg caggaaggcg tcggggatct ggatcaggat gccggcgccg
     6781 tcgccggtat tctcttcgca cccgcacgcg ccgcggtgct cgaggttgac gagcacctcg
     6841 agcgccttgc gcacgatgcc gtacgagcgc cggcccttga tgtggacgac aaagcccaca
     6901 ccgcaggcgt cgtgctcggc ctgagggtcg tacagacctt gggccggggg cgcttcggag
     6961 tggccagtcg cacgcatggg attggacatt gtggcaagcc gagctgcata agtgaaatac
     7021 aatgatctaa gagattcaat ctaactttca catggactcc aggctcagcc gtgcctgcca
     7081 tccaccccgc agagctgcca gcacagcatg cggaaacgcc aatcggctga aaaatggcct
     7141 gattccgtga tttcccccgt atcatgaccc gatatccaag ctgggctggg gcgttcggga
     7201 gcccagccac gggcccagaa cctagagcct atttagagga gtcgtcgaat gcatgcgccg
     7261 atgaagtacg tcgagaaggg tctcgcgctc accgccaacg gtctctggca ggtcttctcg
     7321 accgccaaca ggatcaagca gaacccttcg ttcatcccgc gctggtcgga caagcctctg
     7381 ctcaagtcgt atgagaagac caagccgcag ctgggctggc cgcgcgaaac cgactcgctc
     7441 tgcccgaact gcgtgcgcga ggcgcggcag gagatcctcg acggcaagcg cgacgtctcg
     7501 gtcctgctca acgagaaggt cggcgagatc aaggcgacca tcgtcgagaa ggacggcaag
     7561 atcgtcatgg tgaaggactg ccccatccac ggccacttcg aggacgtgat ggcgatcgac
     7621 acggcgttct tcaagcacct cgaggaggtc ttcccgggcc gcgacatccg cgcgcacaac
     7681 gacgagacgc tccacaacca cggctcgtcc acggtgctgc acggccgcgg ctcggtgctc
     7741 accgtcgatc tgacgaaccg ctgcaacatg atgtgcgacc cgtgcttcat ggacgcgaac
     7801 caggtgggct tcgtccacga gctgacgtgg gaggacatca agcagatcct cgacaacgcc
     7861 atcaccatca agccgcggcg ccagatgtcg gtgcagttct cgggcggcga gccgacgatg
     7921 tcgccgtatt tcctcgacgc gatccgctac tcgcgcaagg tgggctacaa cagcgtgcag
     7981 gcggcaacca acggcatcga gttcgccaag agccccgagt tcgccaaggc ggccgccgag
     8041 gcggggctgc gctacgccta cctgcagttt gacggcatcg gcaacgccgc caactcgcac
     8101 cggcgcgtcg ggaacctctt cgacgtcaag ctccgcgcca tcgagaacct gtaccaggcc
     8161 ggcgtggaca tcgtgccggt catcacgatc gtcaacggcg tcaacaacga gcaggtcggc
     8221 cgcgtcgtcg agttcgcgct cgacaacccg aagcggatca acttcctgtc gttccagccg
     8281 gtgtccttca ccggccgcga cgaagaggtg accccggaac ggcgcgcggc gcagcgttat
     8341 acgctgtcgc acctggcgca cgacgtgaag aaccagaccg gcatcggcga gccgatacgc
     8401 gactggttcc cgatctcgtt catgtccacc ttcaccgact gggccgacgt cgtgcacggt
     8461 cccgatcggg aatggggcca gctgacctgc gggtgccacc cgaactgcgg catcggcatg
     8521 gcagtgatga tcgacaaaga aacgaaggaa gccgtccccg tgacggcgtt cctccacgcc
     8581 gatcaactcg ccaaggacgt cgccaaggtg aacgacgcgg cgcgaggcag gttcctgtcg
     8641 gtcgtcggga tggcgctggc gctgatgcgc aactacgacc cgttcaaggc gccgacgcat
     8701 ttccgcatca tcgacctgat gaagaagttc gacaagacgt tcggcgccac cggcaaggac
     8761 tacggcaagg tgaccggcga ccggacgatg gccgacatcg agaagcggcg gcaggaccgc
     8821 tggaacttcc tcttcatcgc cgggatgtgg ttccaggacc tgttcaacta cgacttccgc
     8881 cgcaccgaga tgtgcatcat cccctacggg acgcagcagg gcgagatttc cttctgcgcg
     8941 tacaacacgg gcatcggctg gcgcaacatc ctcgagaaga tgcacatgac ggccacgctc
     9001 accaagtggt acgacgagca cggccgccac gagatcttcg ccgggaacaa gcacgtgccg
     9061 cttgcgagca aggagcactc gctgcagctc aacgccgagg cggtggcggc gggcgcgcag
     9121 acggacctcg acaagatggg cgtggccaag aacgcgcgcg aggagaagct ggccgctcgc
     9181 aagaaggcgc agatgacgcc cgaagagatc cggcagcacg cggagatgga gcggctgtac
     9241 cggcaggaag tgctcaagga gcagccgacg ctccagatcc aggggctggg cggctccaag
     9301 aagaagcagg acgtccagcc ggtcacgcac aa
//
LOCUS       DBNGSH010000913         2776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3682897, whole genome shotgun sequence.
ACCESSION   DBNGSH010000913 DBNGSH010000000
VERSION     DBNGSH010000913.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3682897"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="AC16CY_20920"
     CDS             <1..440
                     /locus_tag="AC16CY_20920"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3356328.1"
                     /translation="TDALDGLIARVTRQRTSLGAWLDPMADKLLLVSTFVVLTVPAIP
                     LTNHLPLWLTVLVISRDVVIVAVVAIVNLAVGPRTFRPSIWGKAATGTFIVTSVVVMY
                     FNYRREASLLVDLGIWLSLAVTLVSSADYFFRLRRLINDPGTT"
     gene            complement(659..2092)
                     /locus_tag="AC16CY_20925"
     CDS             complement(659..2092)
                     /locus_tag="AC16CY_20925"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HIR3356329.1"
                     /translation="MVRRYNRLLVAFYIVGDIVLAMWAFLLAYGIRFESGLIPVFRGY
                     PPIEQYLRLLPFVAIVTPLAFWVQGVYRLRRGRSRVDDFFAVLVGSVLSVVFGVVSTL
                     YVQTYWASPEARGRGAYEVSQIVWGLFLVLTVMFAYASREAVRELLERRWRAGIGLKR
                     ILIAGAGDLGRMVADRMLQHRELGYHVVGFVDDRATGDHIGYRGLALLGTLSDVAEIS
                     HQERVDHLYVALPPSEHAKVLELMEIASRECIDVKVVPDLLQFIALRARLEDLDGLPI
                     INVNDVPLQGFGAWVKRGLDVVLSAAAMVVLALPALVIAALVKRSSPGPVFYKQERMG
                     LDGRAFTVYKFRSMADDAEFATGPVWARDDDPRITGIGWWLRKLDLDELPQFWNVLKG
                     DMSIVGPRPERPFFVEQFKHRIPQYMLRHKVKAGITGWAQVNGWRGNTSLEKRIEYDL
                     YYIENWSVTLDLKIMWLTLVRGLFQRA"
     gene            complement(2086..>2776)
                     /locus_tag="AC16CY_20930"
     CDS             complement(2086..>2776)
                     /locus_tag="AC16CY_20930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3356330.1"
                     /translation="PVLSRLARWDVATSARVNRFVANSTYVAGRIRRYYNREATIVYP
                     PVDTVFYRPADVTPGNHFLIVSALVPYKRIDLAIEACSALGAALRIVGDGPERDRLQR
                     RAGADTSFLGWLPDEALRDEYQRAAAVIMPGEEDFGIVPLEANACGRPVVALARGGAT
                     ETVEDGDTGVLFTEPTRESLTTALVRARETRFDSIVIRAHAEQFSRERHTERMRAVID
                     ETMAAPVGTRW"
BASE COUNT          487 a          928 c          908 g          453 t
ORIGIN      
        1 tgaccgatgc gctcgacggc ctcattgcgc gggtgacccg ccagcgcacg agcctgggcg
       61 cgtggctcga cccgatggcc gacaagctcc tgctcgtgtc gaccttcgtg gtgctgacgg
      121 ttccggccat tccgctcacc aaccatctgc cgctctggct caccgtgctc gtcatcagcc
      181 gagacgtcgt gatcgtcgcg gtcgtggcca tcgtcaatct cgcggtcggg ccgcggacgt
      241 tcagaccgtc gatatggggg aaggcggcga cgggcacgtt catcgtgacg agcgtggtcg
      301 tgatgtactt caactaccgg cgggaggcgt cgctgctggt ggacctgggt atctggctgt
      361 cgctggcggt gacgctggtc tcgagcgccg actacttctt ccggctccgg cggttgatca
      421 acgatccggg gacgacgtaa atgctggtcc ttggtcctta gagcttggtg cttggtccgt
      481 ccgatggtcc ctggtccgtc ctaggtccct ggttcttggt cttcgtcatt ctgcaaatgt
      541 ttctgcacag tggaccgaca cgggaccaag gcccaaggac ggaccaagga ccaaaggacg
      601 gaccggggac caaaggacgg accggggacc aggaaccaag gacgaaggac ccatctgtct
      661 aggcccgctg gaacagtccc cgcacgagcg tcagccacat gatcttcagg tcgagcgtga
      721 cggaccagtt ttcgatgtag tacaggtcgt actcgatccg cttctcgagc gacgtgttgc
      781 cgcgccagcc gttgacctgc gcccacccgg tgatgcccgc cttgaccttg tggcgcagca
      841 tgtactgcgg gatgcggtgc ttgaactgct ccacgaagaa cggacgctcc ggcctgggcc
      901 ccacgatcga catgtcgccc ttcaacacgt tccagaactg gggcagctcg tccaggtcca
      961 gcttgcgcag ccaccacccg attcccgtga tccgcggatc gtcgtcgcgc gcccagacgg
     1021 gccccgtcgc gaactcggcg tcgtcggcca tcgacctgaa cttgtagacg gtgaacgcgc
     1081 gtccgtcgag ccccatgcgc tcctgcttgt agaagaccgg tcccggcgac gatctcttta
     1141 ccagcgcggc gatcacgagc gccggaaggg ccaggacgac catcgccgcc gccgagagca
     1201 cgacgtcgag accgcgcttg acccaggcgc caaacccctg gagcgggacg tcgttcacgt
     1261 tgatgatcgg cagaccgtcg aggtcttcga gcctggcgcg cagcgcgatg aactgaagca
     1321 gatcgggcac caccttgacg tcgatgcact cgcggctggc gatttccatc agttccagga
     1381 ccttggcgtg ctccgagggt ggaagggcca cgtagagatg atcgacgcgc tcctgatgcg
     1441 agatctcggc gacgtcggac agcgtgccga gcagcgccag cccccgatag ccgatgtgat
     1501 cgcccgtcgc gcgatcgtcc acgaagccca cgacgtggta gccgagctcc cggtgctgga
     1561 gcatgcggtc cgcgaccatc cgccccaggt cgcccgcgcc cgcaatcagg atccgcttga
     1621 gtccgatccc cgcgcgccac cgccgctcga gcagctcgcg gacggcttcg cgcgaggcgt
     1681 aggcgaacat gaccgtcagc acgaggaaga ggccccacac gatctgggag acctcgtagg
     1741 cgccacggcc gcgcgcctcc ggcgacgccc agtacgtctg cacgtagagg gtggacacca
     1801 cgccgaacac gaccgagagc acgctgccga ccagcacggc gaagaagtcg tccacgcggg
     1861 agcgcccgcg ccgcaggcgg tagacgccct gcacccagaa tgccagcggc gtgacgatgg
     1921 cgacaaatgg caggaggcgc aggtactgct cgatcggcgg gtacccccgg aagaccggaa
     1981 tcaggccgct ctcgaatctg atcccgtacg cgagcaggaa cgcccacatc gccagcacga
     2041 tgtcgccgac gatgtagaac gctacgagca ggcggttgta gcgtcttacc atctggtccc
     2101 gacaggcgcg gccatcgtct cgtcgatgac ggcgcgcatg cgctcggtgt ggcgctcgcg
     2161 ggaaaactgc tcggcgtgcg cgcggataac gatcgagtcg aatctcgtct cgcgcgcgcg
     2221 cacgagcgcc gtcgtcagag actcgcgagt gggctccgtg aagagcacgc ccgtgtcacc
     2281 gtcctcgacc gtttccgtcg cgccgccgcg ggccagcgcg acaacgggcc tgccgcacgc
     2341 gttcgcctcg agtggcacga tgccgaagtc ttcttctccg ggcatgatca ctgccgcggc
     2401 ccgttgatac tcgtcccgga gggcctcgtc cggaagccag cccaggaacg aggtgtccgc
     2461 tccggcccgg cgttggaggc gatcgcgctc cgggccgtcg ccgacgatcc gcaacgcggc
     2521 gccgagcgcc gagcacgcct cgatggccag atcgatccgt ttgtagggaa ccagcgcgga
     2581 cacgatgagg aagtgattgc cgggagtgac gtcggcggga cggtagaaca ccgtgtcgac
     2641 gggcggatac acgatcgttg cctcgcgatt atagtatcga cggatccggc cggcaacgta
     2701 agttgagttg gccacgaagc gattgactcg cgccgacgtt gcgacatccc atcgagccag
     2761 cctcgacagc accggc
//
LOCUS       DBNGSH010000914         3104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_797067, whole genome shotgun sequence.
ACCESSION   DBNGSH010000914 DBNGSH010000000
VERSION     DBNGSH010000914.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_797067"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="AC16CY_20935"
     CDS             complement(<1..583)
                     /locus_tag="AC16CY_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3356331.1"
                     /translation="MAEREPKTPVEIADLLQENRSFQPSPSFRSQAIVRDEDVYARAE
                     RDPEAFWAGFAGELEWFTKWTKVLEWTPPHAKWFLGGTLNATVNCVDRHIRGPRRNKA
                     ALVWEGEPGDRRTLTYFDLYRQVSQFANVMKGLGVKRGDRVAIYLPLIPELAIAMLAC
                     ARIGAVHSVVFGGFSAESLRDRINDAQCTLLITA"
     gene            complement(711..1205)
                     /locus_tag="AC16CY_20940"
     CDS             complement(711..1205)
                     /locus_tag="AC16CY_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356332.1"
                     /translation="MQAPLFGGLFIGVLSALPLVGLGNCCCCLWIVSGGVLAAYLDQQ
                     NDPRPTSAGRGALVGLKAGVIGAFVWLIVTLLLQPILEPVQERMTAFVLTMARDLPPE
                     LREALEQSDDASGFRYVIDFLGFLVIGAIFSTLGGLLGASFFKNDVPPALGGPGAPPP
                     PLPE"
     gene            complement(1354..2655)
                     /locus_tag="AC16CY_20945"
     CDS             complement(1354..2655)
                     /locus_tag="AC16CY_20945"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR3356333.1"
                     /translation="MTIAPAGGKLGVLLVGLGAVSTTTIAGVMAIRRGLAKPIGSLTQ
                     MGTIRLGKRTEGRAPRIKDFVPLAGLDDLAFGGWDIFEEDCYAAARTAGVLEPSQLDQ
                     VRTELEAIKPMPAVFDQRYVKRLDGPNVKKGKNKRDLAEQLVEDIRAFKARHGCDRLV
                     MIWVGSTEVYMKETAAHATIASFEKALQENDDSIPSSMIYAYAALREGLPFANAAPNL
                     TADVPAMLQLAGRTKSPVAGNDMKTGQTLIKTIVAPGLKARLLGVRGWYSTNILGNRD
                     GEVLDDPESFKTKEESKKSALEYIFQPHLYPELYSDLCHVVRINYYPPRGDNKEGWDN
                     IDLIGWLGYPMQLKINFLCRDSILAAPIVLDSVLFLDLAKRAGLCGIQEWLSFYFKSP
                     MHAPEVYPEHDLFIQLMKLKNTLRYLQGEDLITHLGREYYD"
     gene            2692..>3104
                     /locus_tag="AC16CY_20950"
     CDS             2692..>3104
                     /locus_tag="AC16CY_20950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3356334.1"
                     /translation="MGRFLRESTVGREPLAVAMSGVRLGERILQIGITDRRVTGALAA
                     KPGLSGESAIVVTDEAAAEVARRAAADAGALVHVAVHALERLPFESGSFDVAVVHAGD
                     AQAAAGVPRAGVLGECRRVLRSGGRIVVLEAGTRTG"
BASE COUNT          565 a          970 c         1020 g          549 t
ORIGIN      
        1 cggcggtaat gagcagcgtg cactgggcgt cgttgatgcg atccctgagt gactcggcgc
       61 tgaacccgcc gaacacgacc gaatgaacgg cgccgatgcg cgcgcaggcg agcatcgcga
      121 tggccagctc cggaatgagc ggcaggtaaa tcgccacgcg atcgccgcgc ttgacgccaa
      181 gccctttcat gacgttagcg aactgcgaga cctggcggta aaggtcgaag taggtcagcg
      241 tgcggcggtc gccgggctca ccctcccaga cgagcgcggc tttgtttcgc cgaggtccgc
      301 ggatgtggcg atcgacacag ttgacggtgg cattcagggt gccgccgaga aaccatttcg
      361 cgtggggagg cgtccactcg agaactttcg tccacttcgt gaaccattcg agctcgccgg
      421 caaaaccagc ccagaaggct tcggggtcgc gctcggcacg ggcatagacg tcctcgtcgc
      481 gcacgatggc ctgcgagcga aacgacgggg acggctgaaa agagcgattc tcctgcagca
      541 ggtcggcaat ctcgaccggg gtcttcggct cccgttcggc cataggtcga acaggataca
      601 tcgccgcgcc actggaaaat ggggtcggga gtcatttatt gaggacagtt tcgcgcagac
      661 gaggatcgcc acgggtcccc ggacagtcat caataaatga ctcccgaccc ctattccgga
      721 agcggcggag gaggtgcgcc cggtccgccc agggccggcg ggacgtcgtt cttgaagaaa
      781 gaggcgccga gcagcccgcc gagcgtggag aagatggccc cgatgaccag gaagccgagg
      841 aaatcgatca catacctgaa tccggacgca tcatcgctct gctcgagcgc ctcgcgcagc
      901 tccggcggca ggtcccgtgc catcgtgaga acaaacgccg tcatacgctc ctggacgggc
      961 tcgagaatcg gttggagcag gagcgtcaca atcaaccaga cgaatgcgcc gatgacaccg
     1021 gccttcaggc cgaccaacgc gccgcgcccg gccgacgtcg gacgagggtc gttctgctga
     1081 tcgaggtagg cggccagtac tccgccgctc acgatccaga ggcagcaaca gcagttgccc
     1141 aggccgacga ggggcagggc ggatagaacg ccgatgaaca atccgccaaa cagtggcgcc
     1201 tgcactactg gtatgtcctt cgcgcatgac taccggccgc tccgctccca ccccaccgcg
     1261 cagacaacgc acggcgggga ccccggttgt cgcggccgga gaatcgcgtt cgaagctcgt
     1321 tgtgcgcggt actcttccag ttgatacagt ccactagtcg tagtactccc gaccgagatg
     1381 ggtaatcaga tcctcaccct gaaggtatcg gagtgtgttc ttcagtttca tcaactggat
     1441 gaacaggtcg tgttcggggt agacctcggg agcgtgcatc gggctcttga agtagaacga
     1501 gagccactcc tgaatcccgc acaggccggc gcgcttcgcc aggtcgagaa acaggaccga
     1561 atcgagcacg atcggcgcgg cgaggatgct gtcacggcac aggaagttga tcttcagctg
     1621 catcggatac ccaagccagc caatcagatc gatgttgtcc catccttcct tgttgtcgcc
     1681 gcgcggcgga tagtagttga tacggaccac gtggcacaga tcgctgtaca actcgggata
     1741 gagatgcggc tggaagatgt actcgagcgc cgacttcttg ctctcttcct tggtcttgaa
     1801 cgactcgggg tcgtcgagga cctccccgtc gcggttcccg aggatgttgg ttgaatacca
     1861 gcctcgaacg cccaggagcc gcgccttgag tccgggcgcc acgatcgtct tgatgagcgt
     1921 ctgccccgtc ttcatgtcgt tgcccgccac gggggacttc gttctgcccg ccagctgcag
     1981 catcgccgga acgtcggcgg tcaggttcgg cgcggcgttg gcgaagggaa ggccttcgcg
     2041 cagcgcggcg taggcgtaga tcatgctcga aggaatggag tcgtcgttct cctggagcgc
     2101 tttctcgaag gacgcgatgg tggcgtgcgc cgccgtttcc ttcatgtaga cctcggtgct
     2161 gccaacccag atcatcacga ggcggtcaca cccgtgccgg gctttgaagg cgcggatgtc
     2221 ctccacgagc tgctccgcga ggtcacgctt gttcttgccc ttcttgacgt tcgggccatc
     2281 gaggcgcttg acgtagcgct ggtcgaacac cgcaggcatc ggcttgatgg cttccagttc
     2341 cgtcctgact tgatccagct gcgacggctc gagcacgccg gcagttcgtg ccgccgcgta
     2401 gcagtcttcc tcgaagatgt cccagccgcc gaacgccaga tcgtccagac cggcgagcgg
     2461 cacgaagtcc tttatcctgg gcgcccgtcc ctcggtccgc tttccgaggc ggatcgtgcc
     2521 catctgggtc agtgagccga tcggctttgc gaggcctcgc cgaatggcca tgacgccggc
     2581 aatcgtcgtg gtgctcacgg cgccgaggcc gacgagcaac acgccgagtt tgccgcccgc
     2641 aggggcaatg gtcactggtg aagtcacaat cgccgactat atcatgagga aatgggcagg
     2701 tttttgcgcg agtccacggt cggacgcgaa ccgctcgccg tggcgatgag cggggtccgg
     2761 ctcggggagc gcatcctcca gatcggcatc acggaccgtc gcgtcacggg tgccctcgcc
     2821 gccaaaccgg gattgtcggg agagtctgcg atcgtggtca ccgacgaagc ggccgcggag
     2881 gttgcgcggc gcgcggccgc agacgcggga gcgctcgttc acgtcgcggt gcatgcgctc
     2941 gaacgccttc cgttcgagag cgggtcattc gatgtcgccg tggtccatgc gggcgacgcc
     3001 caggccgcgg ccggcgtccc gcgcgcggga gtacttggcg agtgcaggcg cgtcctacgc
     3061 tccggcgggc gtatcgttgt gctcgaagca gggacgcgca ccgg
//
LOCUS       DBNGSH010000915         2696 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1305474, whole genome shotgun sequence.
ACCESSION   DBNGSH010000915 DBNGSH010000000
VERSION     DBNGSH010000915.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1305474"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="AC16CY_20955"
     CDS             complement(<1..808)
                     /locus_tag="AC16CY_20955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356335.1"
                     /translation="MTEKRAWLPQYPKVVWVVALVLFGGSFLYRFLEVAFHNDHFEYL
                     ALATEILNGAVPGIDFFDPTRPLQYYASAAGLWFGHQLLAEALLCVTLLSAGAALVFF
                     LGLEISGAVSLGAMASFFVVAILPRLYSYPKIIVPALALLFLWRYLDRPAPSRLAAVA
                     LATAVSFYSRFDYGMWVAFAVGASLLARHWRAWRQLATAAMQYACVVVLLCGPYVVMQ
                     VAMGGGLTSGPGSGRLRHLLQGEDVVSLGLPSIPSERPLVWFRPAGPLATV"
     gene            complement(817..1629)
                     /locus_tag="AC16CY_20960"
     CDS             complement(817..1629)
                     /locus_tag="AC16CY_20960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356336.1"
                     /translation="MATPGPSALSRRQVLERSTHLREDHLRYMQKWGLIRPSHRAHGE
                     VFFAFGDLAIIRQADAELGEGASFRAVLRNLLASRDGQLAFDFRLEASPAKILRLERP
                     EPPPLAALMGDIARLPASTAEQEFAAASALDDGDPEHFDAALAAYRRTLEIDPYLVPA
                     LINLANIHYSRNELVEAQALYERAIAIEPEVFESHFNLGNIFHDLRRYIEARQCYRDA
                     LRINPTYADAHFYLAVTLEKSGHSQEARMHWRAYQELAPNGEWVHLAREFSD"
     gene            1668..2633
                     /gene="glk"
                     /locus_tag="AC16CY_20965"
     CDS             1668..2633
                     /gene="glk"
                     /locus_tag="AC16CY_20965"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR3356337.1"
                     /translation="MLLAGDVGGTKTLLGLFERGSGRPVPVATHSYATTSFATFPDIL
                     DAFARDAGPFRIEAAAVGVAGPVVHEYARLTNVNWHVARDELAARVGGARVHLLNDLE
                     AIAYAVEVLSPDEILVLQQGEPNTVGDAAVIAAGTGLGQAYLRRVNGRLTPAPSEGGH
                     ADFAARTDREMELVRMLRERVGRAEVEQVLSGPGLLNLHRFTHRGGQCQVVDDLSAAD
                     APAAVSRAGLGGRCQGCAEALRMFVSAYGAEAGNLALRGLTLSGLYVGGGIAPKILSA
                     LSNGTFMEAFLAKAPLVDLLAKIPVKVILNAEAGLIGAAICAQEL"
BASE COUNT          463 a          878 c          952 g          403 t
ORIGIN      
        1 ggacggtggc caggggaccc gccgggcgga accacacgag cggccgttcc gaggggatgc
       61 tcggcaaccc gagcgacacg acgtcctcgc cctggagcag atgtctcaat cgtccgctcc
      121 cgggtccgct ggtgagcccg ccccccatcg ccacctgcat cacgacgtac gggccgcaca
      181 ggagcacgac cacgcaggcg tactgcatcg ccgcagtcgc cagttgccgc cacgcccgcc
      241 aatgccgtgc gagcagggat gcgccgacgg cgaaggcgac ccacatgccg tagtcgaatc
      301 gagaataaaa ggacaccgcg gtcgcgagcg cgactgcggc caggcgcgac ggcgcgggcc
      361 ggtccaggta gcgccacagg aacagaaggg ccagggcggg cacgatgatc ttcgggtacg
      421 agtacaaccg gggaagaatg gcgacgacga agaacgacgc catcgctcca agcgacaccg
      481 cgccggatat ctcgaggccg aggaagaaga ccagagccgc cccggccgac agcagcgtca
      541 cgcagaggag ggcctcggcc agcaactggt gtccgaacca gagtcccgcg gcggacgcgt
      601 aatactggag ggggcgagtc gggtcgaaga aatcgatgcc ggggaccgct ccgttcagga
      661 tttcggtggc cagcgccagg tactcgaagt ggtcgttgtg gaaggcaacc tcgaggaacc
      721 ggtacaggaa cgacccgccg aacaacacga gcgcgacgac ccagacgact ttcgggtact
      781 ggggcaacca ggcgcgcttc tccgtcatct gatctctcag tcggagaact ccctggccag
      841 gtgcacccac tccccgttgg gcgccagctc ctgatacgcg cgccaatgca tgcgggcctc
      901 ctgcgagtgc ccgctcttct ccagcgtcac ggccaggtag aagtgcgcgt cggcgtaggt
      961 cgggttgatg cggagcgcgt cgcggtagca ctgccgcgcc tcgatgtagc ggcggaggtc
     1021 gtgaaagatg ttaccgaggt tgaagtgcga ttcgaacacc tccggttcga tcgcgatcgc
     1081 acgttcgtac aacgcctggg cctccacgag ctcgttgcgc gagtagtgga tgttggcgag
     1141 gttgatcaac gccggcacca ggtaagggtc gatttcgagc gtgcgccggt aggccgccag
     1201 cgccgcatcg aaatgctccg gatcgccgtc gtccaaggct gacgccgccg cgaattcctg
     1261 ttcggccgtg gaggccggaa gtcgcgcgat gtcgcccatc agcgccgcca acggcggcgg
     1321 ctcggggcgc tcgagccgga ggatcttcgc cgggctcgcc tcgaggcgga aatcgaacgc
     1381 gagctggccg tcgcgcgacg cgagcaggtt tcgcagcacc gcgcggaagc tggcgccctc
     1441 gccaagctcg gcatctgcct gcctgatgat ggccagatcg ccgaaggcga agaagacttc
     1501 gccgtgcgcg cggtgcgaag ggcggatgag cccccatttc tgcatgtagc ggaggtgatc
     1561 ctcgcgcagg tgcgtggatc gctcgagcac ctggcgacgc gagagagcgc tgggaccagg
     1621 agtagccacg gcgtcatcac tataaactac atgcagcttc actcatcatg ctgcttgctg
     1681 gcgacgtcgg cggtaccaag acgctgctcg gcctcttcga gcgcggcagc ggccgtccag
     1741 tgccggtcgc cacgcacagc tacgcgacga cgtcgttcgc cacctttccc gacatcctcg
     1801 acgctttcgc gcgagacgcg gggccgttca ggatcgaggc ggccgcggtg ggcgtggcgg
     1861 gcccggtcgt gcacgagtac gcccggctca ccaacgtcaa ctggcacgtc gcccgcgatg
     1921 aactcgcggc ccgtgtgggc ggcgcccgcg ttcacctgct gaacgatctc gaggcgatcg
     1981 cgtacgccgt cgaggttttg tcgccggacg agatcctcgt tcttcagcag ggggagccga
     2041 atacggttgg cgacgcggcg gtgatcgcgg cgggcaccgg cctggggcag gcctatctgc
     2101 ggcgcgtgaa cgggaggctg acacccgccc cctccgaggg cgggcacgcg gacttcgcgg
     2161 cgcggaccga tcgcgaaatg gaactggtcc ggatgcttcg cgaacgcgtc ggccgcgccg
     2221 aggtcgagca ggtgctcagc gggccgggcc tgttgaacct ccaccgtttc acgcaccgtg
     2281 gcggacagtg ccaggtcgtg gacgatctga gcgcggcgga cgctcccgcc gccgtctccc
     2341 gggcaggcct gggaggccgc tgtcaggggt gcgcggaggc gctccgcatg ttcgtcagcg
     2401 cgtatggcgc tgaggcaggg aacctcgcgc ttcgagggtt gacgctgtcg ggcctgtacg
     2461 tgggcggcgg catcgcaccg aagatcctct cggcgctctc gaacggtacg ttcatggagg
     2521 cgttcctggc caaggcgccg ctcgttgacc tgctcgcgaa gatcccggtg aaggtcatcc
     2581 tgaacgcaga ggccgggctg atcggcgcgg cgatctgcgc ccaggaactg tgatagaagg
     2641 gggctgtcgt catgcgcacg cgcattcggg acctggccgt cgtcatcgcc gccgcg
//
LOCUS       DBNGSH010000916         4282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_797364, whole genome shotgun sequence.
ACCESSION   DBNGSH010000916 DBNGSH010000000
VERSION     DBNGSH010000916.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_797364"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1026)
                     /locus_tag="AC16CY_20970"
     CDS             complement(100..1026)
                     /locus_tag="AC16CY_20970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3356338.1"
                     /translation="MKRVLVLGTLIAVGALSIVASGQAPQGPSAEAKAAARIERVRGN
                     LYVITGSGAENQALFSGGNTAVFITETGVTLVDTKLPGFGPTILERIKTVTDKPVTRI
                     INTHSHGDHTGGNLFFGTTVESIVHENAKASMAKAEYSGDNAKFLPKRTYTTTLTLGS
                     GSDRIDLYHFGRGHTNGDTFIVFPALRTMHAGDMFAWKALPYVDAGNGGSVVAQPDSL
                     ARAAAVPNVETVIPGHIPVATINELKEYASFTRDFVNYARASMKAGKTVEQAAKEYTV
                     DPKYKGYVVSVNPAFGSAQANLEIAYTELKGK"
     gene            1192..1746
                     /locus_tag="AC16CY_20975"
     CDS             1192..1746
                     /locus_tag="AC16CY_20975"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR3356339.1"
                     /translation="MNGHGHCSSPRVGVHAARRLSDFSYQGPHSYSVTCCTFARRPSF
                     QHDGIVASSSEQLLQLSLQEHFAVLAYCFMPDHVHLLVEGLQASSDLRRLMARWKQRT
                     GYDHSRATGERLWQAGFFDHVLREEEDRLSVVAYILANPVRAGLVTHPSDYPSWGSSV
                     WTRAELIESLFNRHGGESDNHRGG"
     gene            complement(1867..2952)
                     /locus_tag="AC16CY_20980"
     CDS             complement(1867..2952)
                     /locus_tag="AC16CY_20980"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate
                     methylesterase/protein-glutamine glutaminase"
                     /protein_id="HIR3356340.1"
                     /translation="MTPTHAAKTLSVLVVDDSAVMRQVMTTVLSREAGIHVETAAHPL
                     IAVGKMKQRRPDVILLDIEMPHVDGLTFLQQIMADEPIPVVVCSALAQGGADTVLRAL
                     EYGAVDVVQKPAVGIQDFLYESAIMLVDKVRGAAQARPRRRAAVSQVERRHTADAVLP
                     TMAGRSLRVTTDKVVAVGTSTGGTEALRTLLMTLPHDSPGLVVVQHMPEVFTAGFAER
                     LNQICQVDVKEASTGDRVRPGCVLIAPGNRHTTIVRNGAQYAVQVVDGPLVSRHRPSV
                     DVLFRSVAQSAGSNAVGVLMTGMGDDGAEGLLEMKRAGAATIAQDEATSVVFGMPKAA
                     IDRGAVDEVLPLPRIAGAILAKAVAKR"
     gene            complement(2976..3878)
                     /locus_tag="AC16CY_20985"
     CDS             complement(2976..3878)
                     /locus_tag="AC16CY_20985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR3356341.1"
                     /translation="MSPARPVPSVEIHVREIDDGLFERFRALIYREAGIALGDGKKSL
                     FISRVAGRLRELGLSSFEQYYRIVADPDQVEERGCLLDRICTNETQFFRDPRQWQFLD
                     EHAFARFESEMDGQGVRRIRAWSAACSSGEEPYSLAMALLNRFQPSAGWRVEVVGTDL
                     STKVLALARAGVWPIEKSSDIPASYRRAFMLKGTGSQSGRMKAGPELRDVVSFARLNL
                     NDAVYPVTGQFELIFCRNVLIYFDRESKSRVIDRLLTHLAPGGYLFMGYAETTATISD
                     RLESVGPNVYARVGERTRDPGRTP"
     gene            complement(3875..>4282)
                     /locus_tag="AC16CY_20990"
     CDS             complement(3875..>4282)
                     /locus_tag="AC16CY_20990"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR3356342.1"
                     /translation="ATPPYIRGVINLRGSVVPVVDLSVKFNLPVHPVSRRTCIVIMET
                     TILGERATMGIVADSVNQVIEFSPQDIEAAPAFGTGIRLEYLEGMAKIGTKFVLVLNM
                     DEILSSADAPVLPAAGAQTAQQAAAAAREAQIS"
BASE COUNT          669 a         1445 c         1412 g          756 t
ORIGIN      
        1 cggtctatct gcgcggcacg gaggtcaacc gcgctgcgct gcgatgaccg gcctggcgtc
       61 ggcgcgcgca cggccaccgc gggggctcaa gcccccgctc tacttccctt tcaactcggt
      121 gtaggcgatc tccaggttgg cctgcgcgct gccaaacgcg gggttgacgc tcacgacgta
      181 gcccttgtac ttcgggtcga ccgtgtactc cttcgcggcc tgctcgaccg tctttcccgc
      241 cttcatggac gcgcgcgcgt agttgacgaa gtccctcgtg aagctcgcgt actccttcaa
      301 ctcgttgatg gtggcgaccg gaatatgccc gggaatcaca gtctccacgt tcggcacggc
      361 ggcggccctc gcgagcgagt cgggctgcgc cacgacgctt ccgccgttgc cggcatcgac
      421 gtagggaagc gccttccacg cgaacatgtc gccggcgtgc atcgtccgca gcgccgggaa
      481 gacgatgaag gtgtcgccgt tggtgtggcc gcggccgaag tgatacaggt cgatccgatc
      541 gctgccggag ccgagcgtca gcgtggtcgt gtaggtccgc ttcgggagga atttcgcgtt
      601 gtcgccgcta tactccgcct tcgccatgct cgccttggca ttctcgtgaa cgatgctctc
      661 gacagtagtg ccgaagaaca ggttgccgcc cgtgtgatcg ccgtgggagt gcgtgttgat
      721 gatgcgcgtg accggcttgt cggtgaccgt cttgatcctt tcgaggatgg tcgggccgaa
      781 gccgggcagc ttcgtatcga cgagcgtgac gccggtctcc gtgatgaaca ccgcggtgtt
      841 gccgcccgaa aacagcgcct ggttttccgc gccggagccg gtgatcacgt agaggttgcc
      901 cctgaccctt tcgatacggg ccgccgcctt cgcttcggcc gatggcccct ggggcgcctg
      961 ccccgacgcg acgatggaca acgctccaac cgcaatcaac gtgccgagca cgagcactcg
     1021 cttcatgacg tcgctccggc gctcattcta gcgccgtcat tcaatcaccg cggggctaat
     1081 tcgatcacgc gggggctgaa gcccccgccc tacgacggtc gggcgttggc cgccccgcag
     1141 ccgtagagcg cacgcttcag cgtgcgcgat cacgcggtac ggcctcgttc gatgaacggg
     1201 cacgggcatt gctcctcgcc acgcgtgggt gtccatgcgg ctcgccgctt gtcggatttt
     1261 tcctaccagg ggccgcattc gtactcggtt acctgctgca ccttcgcgcg ccgtccgtcg
     1321 tttcagcacg atgggatcgt tgcctcgtcg tctgagcagc tcctgcaact gtcactgcag
     1381 gaacacttcg cagttcttgc gtactgcttc atgccagacc atgttcactt gctggtcgag
     1441 ggactgcagg catcgagcga cctccgcagg ctgatggcgc ggtggaagca gaggactggg
     1501 tacgaccact cacgggcaac gggggagcgc ctgtggcagg ccggcttctt cgaccacgtg
     1561 ctgcgggagg aagaggatcg cctctccgtt gtggcctaca ttctggcgaa ccctgtgcgg
     1621 gccgggctcg tgacgcaccc gtccgattac ccgtcgtggg gatcgagtgt gtggacccgg
     1681 gcggagttga tcgaatcgct gttcaaccgc cacggaggcg agagcgacaa ccaccgcggg
     1741 ggctgaagcc cccgctctac gacggtcggg cgttggccac cccgcaaccg tagagcgcac
     1801 gcttcagcgt gcgcggatcg gcccagcgtg cgccgatcgg cccagcgtgc gcggatcagg
     1861 ggcgcgtcag cgcttcgcca cggccttcgc gaggatcgca ccggcgattc ggggcagggg
     1921 cagcacttcg tccacggcgc cgcggtcgat ggcggccttg ggcatgccga agacgaccga
     1981 ggtcgcctcg tcctgcgcga tggtcgccgc gccggcgcgc ttcatctcca gcaaaccctc
     2041 cgcgccgtcg tcccccatcc cagtcatcag gacacccacg gcgttgctcc cggccgactg
     2101 ggcaaccgat cggaacagca cgtccacgct gggccggtgc ctcgacacga gcgggccgtc
     2161 cacgacctgg acggcgtact gagcgccgtt gcggacgatc gtcgtgtgcc ggtttcccgg
     2221 agcgatgagc acgcagcccg gccggactcg atcgccggtg gacgcctctt tcacgtccac
     2281 ctggcagatc tgattcagcc gctccgcgaa gcccgccgtg aagacctcgg gcatgtgctg
     2341 tacgacgacg agcccgggcg aatcgtgcgg aagcgtcatg agcagcgtgc ggagcgcctc
     2401 ggttcctcct gtcgaggtcc cgaccgcgac gaccttgtcc gtcgtgaccc tgagcgagcg
     2461 gccggccatc gtcgggagga cggcgtccgc cgtgtgacgc cgctccacct gtgacaccgc
     2521 cgcgcgcctc cgcggccgcg cctgagcggc gccgcgcacc ttgtccacga gcatgatggc
     2581 cgattcgtac aggaagtcct gaatgcccac cgcgggcttc tgcacgacgt ccacggcgcc
     2641 gtactcgagg gcccggagca cggtgtccgc cccgccttgc gcgagggcag aacagaccac
     2701 gaccgggatc ggctcgtccg ccatgatctg ctgaaggaac gtgagcccgt ccacgtgcgg
     2761 catctcgata tcgagcagga tcacgtccgg ccggcgctgt ttcatcttcc ccacagcgat
     2821 gagcgggtgc gcggccgtct cgacgtgaat ccccgcttcg cgcgacagga cagtcgtcat
     2881 cacctgccgc atcacggcgg aatcgtccac gacgaggacg ctcagcgtct tggctgcgtg
     2941 cgtcggtgtc atgccggcgt tcctcctcga tgttctcagg gcgtacgtcc ggggtcccgt
     3001 gtccgctcgc ccacgcgggc gtatacattc gggccgacgc tctcgagacg atccgagatg
     3061 gtggccgtcg tctcggcgta gcccatgaac aggtaaccgc ccggcgccag atgcgtcagc
     3121 agtcgatcga tgaccctcga cttcgattcg cgatcgaagt agatgaggac gttgcggcag
     3181 aagatcagct cgaactgacc ggtgaccgga tacaccgcat cgttgagatt gagccgcgcg
     3241 aagctcacca cgtcgcggag ctccggaccc gccttcatcc tgccgctctg gctgccggtt
     3301 cccttcagca tgaaggccct gcggtatgac gcggggatgt cggacgactt ctcaatcggc
     3361 cagacgccgg cgcgggccag ggcgaggacc ttggtggaca ggtccgtccc gaccacttca
     3421 acccgccacc cggcggacgg ctgaaaccgg ttgagcaacg ccatcgccag cgagtacggc
     3481 tcctcgccgg aggagcaggc ggcgctccac gctcgaatcc gcctgacgcc ttgcccgtcc
     3541 atttccgact cgaagcgggc aaaggcgtgt tcgtcgagga actgccactg gcgagggtcg
     3601 cggaagaact gggtttcgtt cgtgcagatg cgatcgagca ggcacccgcg ctcctcgacc
     3661 tggtcgggat cggccacgat gcggtagtac tgctcgaagg acgagaggcc cagctctcga
     3721 agccttccgg ccacccggct gatgaacagc gatttctttc cgtccccgag cgcgattccg
     3781 gcttcgcggt agatgagcgc ccggaaccgc tcgaacaagc cgtcgtcgat ctcccggacg
     3841 tggatctcga cggagggaac gggccgggcc gggctcacga tatctgggcc tctcgagccg
     3901 ccgcggccgc ctgctgcgcc gtctgggcgc ccgccgccgg aaggaccggg gcatcggcag
     3961 aggagaggat ctcgtccatg ttcagcacga ggacgaactt ggtcccgatc ttggccatgc
     4021 cttcgaggta ctcgagccgg atgccggtgc cgaacgctgg tgcagcctcg atgtcctgcg
     4081 gcgagaactc gatgacctgg ttcaccgagt ccgccacgat gcccatcgtg gcgcgctcgc
     4141 cgagaatcgt cgtttccatg atgacgatgc aggtgcggcg gctgaccggg tgcaccggca
     4201 ggttgaactt gacggacaga tcgacgacgg gcacgacgct cccgcgcagg ttgatgacgc
     4261 cgcggatgta gggaggtgtc gc
//
LOCUS       DBNGSH010000917         1971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2664934, whole genome shotgun sequence.
ACCESSION   DBNGSH010000917 DBNGSH010000000
VERSION     DBNGSH010000917.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2664934"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="AC16CY_20995"
     CDS             <1..468
                     /locus_tag="AC16CY_20995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3356343.1"
                     /translation="FNGHYRRIARVILRVIQDPSRAEELAVEVFWKLWRRPSAQGPQC
                     AGWLYRTAVRAAVDELRKRSRREKYERWFGVRGPSGDPEAAREATEQRTRVRTVLAAL
                     RSRDAQLIALRSEGLSYEELAEALQMKPASVGTILRRAHESFRQEYIRRYGED"
     gene            455..1384
                     /locus_tag="AC16CY_21000"
     CDS             455..1384
                     /locus_tag="AC16CY_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356344.1"
                     /translation="MEKIDRLEEWVHQRMAAPEPAAAWPDPAAGRRLLDRRIAARQRP
                     LMLWAGAAAALCAAVLVLPGPRLAAQRLWDRVVLGRIQVLIADLDVAGAASGFFSPQN
                     LRLDVRSASSIGEASERAGFWPRLPDPDVFPGSPTYSVADATLATLPLRTPAIRYLVA
                     RAGGSPGEVPDSWNGAMLEVRVGPVILADYGGVVLLQSLPFELVKPVDFDLELFYRIA
                     FQALGASELYAQSLGADLAVNPALLMFMPREDSHLVHEFPTRSGNGVMIGEVYGPGSK
                     VALWSGSDRLYALFSGTGKVTSDFLIRVANTLD"
     gene            complement(1398..1805)
                     /locus_tag="AC16CY_21005"
     CDS             complement(1398..1805)
                     /locus_tag="AC16CY_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005193455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3356345.1"
                     /translation="MLAVVDTGPLYAAVDADDADHARCRAVLEDGRYSVIIPTLIVAE
                     ATYLIGTRLGPAVEARFLKSLSVMDVEAPIPDDWIRIAELVEHYGDFPLGGADASVVA
                     LAERLDAEVIVTLDERHFRAVRPRHRKAFRLVP"
     gene            complement(1789..>1971)
                     /locus_tag="AC16CY_21010"
     CDS             complement(1789..>1971)
                     /locus_tag="AC16CY_21010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356346.1"
                     /translation="RRHDTSVSDVVRRAVSAFLGVSEGGRRELPFAALGRSGRRHTAR
                     DAEKILTAEWGHARRR"
BASE COUNT          297 a          657 c          678 g          339 t
ORIGIN      
        1 ttcaacggcc actaccggag gatcgcgcgc gtcatcctcc gggtcattca ggatccctcc
       61 cgcgccgagg aactggcggt cgaggtgttc tggaaactgt ggcgccggcc atccgcgcag
      121 ggaccgcagt gcgcgggatg gctctaccgg acggccgtcc gggcggcggt ggacgaactc
      181 cgcaagcgat cgcggcgcga gaagtacgag cgctggttcg gtgtcagagg cccctcgggc
      241 gatcccgaag ccgcgcggga ggccaccgaa cagcggaccc gcgttcgcac ggttctggcg
      301 gcgcttcgat ccagggatgc gcagctcatt gctctccgtt cagagggtct gtcctacgag
      361 gagctggccg aggcgctgca gatgaagcct gcgtcggtgg gcacgatcct gcgccgcgcg
      421 cacgaatcat tccgacagga gtacatcaga cgctatggag aagattgatc gtctcgaaga
      481 gtgggtgcac cagcggatgg ccgcgccgga gcctgcggcc gcgtggccgg accccgcggc
      541 cgggaggagg ctcctcgacc ggcggatcgc ggcgcggcag cggcccttga tgctctgggc
      601 cggcgcagca gcggcgttgt gcgcggccgt gctcgtgctg ccgggacccc gcctcgcggc
      661 tcagcgactc tgggaccgtg tggtcctcgg caggatccag gtgttgattg cggatctcga
      721 tgtagccggt gcagcgtcgg gcttcttctc tccgcagaat ctacggctcg acgtccgttc
      781 cgcgtcgtcg atcggcgagg ccagtgagcg cgctggcttc tggccccgcc tgcccgatcc
      841 tgatgtgttc ccgggttcgc cgacctattc ggtcgccgac gccacgctgg ccactctgcc
      901 gctccgcacg ccggcgatcc gctacctcgt cgcccgggcc ggaggatcac ccggcgaggt
      961 gcccgattcg tggaacggcg cgatgctcga ggtccgcgtg ggccccgtga tcctggccga
     1021 ctacggcgga gtcgtgctgc tgcagagtct tcccttcgag ctggtcaagc cggtggactt
     1081 cgacctcgag ctgttctacc gaatcgcatt tcaggcgctc ggcgcgagtg agctgtatgc
     1141 gcagtcgctt ggcgccgatc tcgcggtcaa tcctgccctg ctcatgttca tgcccaggga
     1201 agacagccat ctggtgcacg agttcccgac caggtcaggc aatggcgtca tgatcgggga
     1261 ggtctacggg cccggatcga aagtcgcctt gtggagcggt tccgaccgtc tgtatgccct
     1321 cttctcgggc actgggaagg tgaccagcga tttcctgatc cgcgtggcga atactctgga
     1381 ctaatggtgc cggtccgcta cggcacgagg cggaacgcct tgcggtgtct cggacgcacg
     1441 gcgcggaagt ggcgttcatc cagggtcacg atcacctccg catcaagacg ttccgcgagc
     1501 gcgacgacgg aggcgtcagc gccacccagc gggaaatcgc cgtagtgctc gaccagttca
     1561 gcgatccgga tccagtcgtc ggggatcggc gcctccacgt ccatgactga caggctcttc
     1621 aggaatcgcg cctcgacggc gggtcccagc cgtgtgccaa tgaggtaggt ggcttcggcg
     1681 acgatcagcg ttggaatgat cacgctatac cggccgtcct cgagcacagc ccggcatcgc
     1741 gcatgatcgg catcgtcggc gtccacggcc gcatagagcg gcccggtatc aacgacggcg
     1801 agcatggccc cattcggccg tcaggatctt ctcggcgtcg cgcgcggtgt gacgccgccc
     1861 gctgcgtccg agtgccgcaa acggcagctc gcggcgcccg ccctccgaga cgcccaggaa
     1921 cgccgagacc gctcggcgca cgacgtcgga caccgacgtg tcgtgccgcc g
//
LOCUS       DBNGSH010000918         2814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4533092, whole genome shotgun sequence.
ACCESSION   DBNGSH010000918 DBNGSH010000000
VERSION     DBNGSH010000918.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4533092"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="AC16CY_21015"
     CDS             complement(<1..1634)
                     /locus_tag="AC16CY_21015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3356347.1"
                     /translation="MRTRRAALTAAAIAVVGTGALVARPLPQGQDPTVFRGRVQSIEV
                     DVRVTDVNGNAVRGLTKDDFTLLEDDKPQQIITASFVDLERESPVTPMVPGVVESDVA
                     TSAGVGRLWVILLGSTIGPGGRAGRARFVAGKFVQESLGPNDQVAVISIHGTMKSAQA
                     FTRNRTLLLNAIERLDEEPPNPADDAIRTAYQVLEDVCNRLGRLVGRKAVLYFDPPTF
                     FNIAGPPPAGSSAISKDVGYFFDQRDAIAAATRNNVAIYVVSTVGIAGATIDGSTAGG
                     SLPPSTIQAVAGQRLLAEETGGDAIVNTNNYQVGYERFVQDSNQYYLLGYTPQVEHRD
                     GKLHRLTVRVNRPGLTVRARQGYYAPRPERRNARETKPEQIAGAGKLSSAAIDALQMP
                     LSMNGLTLDLFAAPFRAASGKNATVLVGAQVRGRGLVLSRGERMEIGFVGMNTDGKTS
                     PGAFHIVRLDLTDHSRQVVEGSGLPFVDRLQLAPGRHQVKFVAHQPTGQTGMVLVDVD
                     VPKFSDAPLSMSGVVLASFLELPQTAVKRDDPLLRLL"
     gene            complement(1688..2083)
                     /locus_tag="AC16CY_21020"
     CDS             complement(1688..2083)
                     /locus_tag="AC16CY_21020"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3356348.1"
                     /translation="MKGFLVAWMSALVIVAAAPAIAHHSSAGIDRAKSVTLTGTIRQF
                     SWSNPHSWMEVDVTDEKGAIVTWKVEMTSPAYLVRAGWKSTTLKQGDEVKVTVRPLRD
                     GSPGGLFVSVTLADGRSFTEKPAIPATAK"
     gene            complement(2104..>2814)
                     /locus_tag="AC16CY_21025"
     CDS             complement(2104..>2814)
                     /locus_tag="AC16CY_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356349.1"
                     /translation="PSVAPVVGALAPANLAKPRPKPPFDLTGTWFIDFNSPNSWRFGP
                     PTTKLTPQAQVHFDAARKAQAEGKAYRDDIGQCWPAGLPLIMTRVWPIAMIQLPTAIY
                     MVSHFMNSLRIIYMDGRPHSDPDIVVRTFNGESIGRWEGDTLVVDTKHFVNDHHWIDS
                     GVPASDALHIVERIRLINKGATLEIEYTMTDPKSWEGEWKYLKRWNRVDDQDITEVEC
                     LPDLNSHIPSTQSEFQAR"
BASE COUNT          468 a          923 c          910 g          513 t
ORIGIN      
        1 agaagccgca acagcgggtc gtcccgcttc accgccgtct gcggcagctc gagaaacgag
       61 gcgagcacga cgccgctcat cgacagcggc gcatccgaga atttcggcac gtcgacatca
      121 acaaggacca tccccgtttg cccggttggt tgatgcgcga cgaacttcac ctgatgacgt
      181 cccggtgcca gttgcagccg gtcaacgaac ggcaatccac tcccctctac cacctgacgt
      241 gagtggtcgg tcagatccag cctgacgatg tggaacgcgc ccggcgatgt cttgccgtcc
      301 gtgttcatgc ccacaaagcc gatctccatc cgctcgcctc ggctgaggac gaggccgcgg
      361 ccacgcacct gggcgccgac caggaccgtc gcgttctttc ccgacgcagc gcggaagggc
      421 gccgcaaaca gatcgagcgt caggccgttc atcgacaacg gcatctggag ggcgtcgatg
      481 gctgccgagg agagcttgcc cgccccggcg atctgctccg gcttcgtctc gcgcgcattg
      541 cgccgctcgg gtcgaggcgc gtagtacccc tgtcgcgcgc gcacggtcaa cccggggcga
      601 ttcacgcgaa cggtcagacg atggagcttg ccgtcacggt gctccacctg aggggtgtag
      661 ccaagcaggt agtactggtt gctgtcctgg acgaagcgct catagccgac ctggtagttg
      721 ttcgtgttga cgatcgcatc accccccgtc tcctctgcga gaagacgctg cccggcaacg
      781 gcctggattg tgctgggtgg caggctgcca ccggccgtcg atccgtcaat ggtcgcgcct
      841 gcgatgccga cggtcgagac cacataaatc gcaacgttgt ttcgcgtcgc cgctgcaatc
      901 gcgtcccgct ggtcgaagaa atagccgacg tccttggata tggcgctcga gcctgccggc
      961 ggcggtcccg cgatgttgaa gaaggtcggc ggatcgaaat acagcacggc cttgcgaccg
     1021 acaagacgtc cgagacgatt acagacgtct tccagtacct ggtacgccgt gcgaatggcg
     1081 tcgtcggcgg gattgggcgg ctcctcgtcg agtcgttcga ttgcgttgag gagcagcgtg
     1141 cggttgcgcg tgaacgcctg ggcgctcttc atcgtgccgt ggatgctgat gacggccacc
     1201 tgatcgtttg gtccgagtga ctcctggacg aactttccgg cgacaaatcg cgcgcgcccg
     1261 gcccggccgc caggcccgat cgtgctgcca agcaggatga cccacaggcg cccgacgccg
     1321 gcgctggtgg caacgtcgga ttcgacaacg ccagggacca tgggcgtcac gggcgattca
     1381 cgctccagat cgacgaagga ggcggtgatg atctgctgcg gcttgtcgtc ctcgagcagc
     1441 gtgaagtcgt ccttcgtcaa gcctcgaacg gcgtttccgt tcacgtcggt gacgcggacg
     1501 tccacttcga tcgactgaac gcggccgcga aagacggtgg ggtcctgacc ttgcggcagg
     1561 ggacgggcga cgagcgcgcc ggtcccgacg acggcgatgg cggctgcggt gagagctgcg
     1621 cgtcgcgtcc gcatacgtcg ttctctcctt accgatgcgc cggagcctca cggggaggcc
     1681 tccgatgcta tttcgcagtc gcgggaatcg caggcttctc ggtgaacgag cggccgtcgg
     1741 ccagcgtgac cgacacgaac agcccgcccg gcgaaccatc ccgcagcggg cgcaccgtga
     1801 ccttcacctc gtcgccctgc ttcagcgtcg tggacttcca cccggcgcgg accagatacg
     1861 ccggcgacgt catctcgacc ttccaggtga cgatggcccc cttctcgtcc gtgacgtcca
     1921 cttccatcca cgagtgcggg ttcgaccacg agaactgacg gatggtcccg gtcagcgtga
     1981 ccgacttcgc gcgatcgatg ccggccgacg agtgatgtgc gatggcgggc gccgccgcga
     2041 cgatcaccag cgccgacatc cacgcgacga gaaagccttt catgagcgtc ctccggccgt
     2101 gtctcaacgg gcctgaaact ccgattgcgt gctgggaatg tgcgaattca ggtcgggcag
     2161 gcactccact tccgtgatgt cctgatcgtc cacgcgattc caccgtttca agtacttcca
     2221 ctccccttcc cagctcttcg gatcggtcat cgtgtactcg atctccagcg tcgcgccctt
     2281 gttgatcagc cggatgcgct cgacgatgtg cagggcgtcg ctggctggca cgcccgaatc
     2341 gatccagtgg tggtcgttga cgaagtgctt cgtgtcaacg accagcgtgt ccccttccca
     2401 tcgtccgatg gactcgccgt tgaacgtgcg gacgacgatg tcgggatcgg agtggggcct
     2461 gccgtccatg tagatgatcc gcaggctgtt catgaagtgg ctgaccatgt agatcgccgt
     2521 cggcagttga atcatcgcga tcggccagac gcgcgtcatg atgagcggca gacccgccgg
     2581 ccagcactgt ccgatgtcgt cgcggtaggc cttgccttcg gcctgcgcct tcctcgccgc
     2641 gtcgaagtgc acctgcgcct gcggcgtgag cttcgtcgtc ggcggaccga agcgccagga
     2701 gttcgggctg ttgaagtcga tgaaccacgt gccggtgagg tcgaacggcg gcttcgggcg
     2761 cggcttcgcc agattcgccg gcgccagcgc gccaacaacg ggcgcgacgg acgg
//
LOCUS       DBNGSH010000919         2924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3345065, whole genome shotgun sequence.
ACCESSION   DBNGSH010000919 DBNGSH010000000
VERSION     DBNGSH010000919.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3345065"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..527
                     /locus_tag="AC16CY_21030"
     CDS             78..527
                     /locus_tag="AC16CY_21030"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR3356350.1"
                     /translation="MKARRLSLAAVAILALAAPAFAQQPSPAGRVKVVSGSAFIVRAG
                     STVPAKVGQELFESDSLRTGDDGRIGVTFEDDTRLSLGPASEVRLDTFRYAPAEGSLG
                     MAMRFLRGVAVYVSGKIAKLAPDSIRLETPSAIVGVRGTTLAINVRE"
     gene            524..1156
                     /locus_tag="AC16CY_21035"
     CDS             524..1156
                     /locus_tag="AC16CY_21035"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3356351.1"
                     /translation="MTRAWGGVVLVAVTACLNAACGPQRPRTPQRSGQDLVVLLPNDD
                     GTTGKVEVSNPSGRVTLDTARASTLIAVGQAPGEVTELTEAEIQRIFGPALAAQPPAP
                     IQFTLYFQFDSEELTDDSRQMLSEVLQVVKKYPVPRVTVVGHTDTTGSAESNVELGLR
                     RANAVRALLVDVGVEGAAIDSTSHGEAALLVPTADGVFEPKNRRVDVTVR"
     gene            1153..>2924
                     /locus_tag="AC16CY_21040"
     CDS             1153..>2924
                     /locus_tag="AC16CY_21040"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3356352.1"
                     /translation="MSPRHRRILLLCGLLPTLTAAILSLYRPASFAGLEYAAYDTLVR
                     WADTRPPSGRVLIVDVDERSLASVGQWPWRRDVVGRLIDGLRSRGAATVALDIMFPEA
                     DRFEGSGRSPDAALAKTLHQGRVILGYGLLFASTAGAPSPCLRHALGLPIVRGIGAPT
                     DDPFFRATSAVCNIPVLTDASDASGFLNAAPDPDGILRRAPLLMQYADRIYPALSLAA
                     VRAVTDANDLLLRVANVNTSSLTLAGQAVPLDGKSNLLLRYRGPKGTFRYVSAVDVLY
                     GKTDPDVFKDKIVFVGTTALGTREVVATPLDTLFAGVEVQATLADNLLQRDFIYRPEH
                     GVTLETQAVIGLGAIVALVVARFGLVWGALAAGSFVASIWIGADSLLASDGAYFSPLY
                     PTLALTTGLAAMTVAAFGVERRRAERAGREKESSRRLMVQTLLSLTGIRDFETGRHSL
                     RTQKYTKVIAEELSKHPDFRAYLTPERVELLASLAPLHDIGKVGVPDRVLNKPGLLTP
                     DELTEMRRHPRYGRDVIENAERLAGVRDDVALAVAKDIVYTHHEWWDGSGYPEGLRGT
                     AIPIPGRVMAVVDVYDAIRTRKLY"
BASE COUNT          450 a         1029 c          955 g          490 t
ORIGIN      
        1 aggagccacg ccgtccttgt tctcggcggc cgtctctggc acagtagaat tctgcccccc
       61 cggatgtgga gtgtcatatg aaggcccgtc gcctctcgct cgccgccgtc gcgatccttg
      121 cgctggctgc accggcgttt gctcagcagc cgtcgccggc agggcgcgtc aaggtggtct
      181 ccggctccgc gttcattgtc agggctggct cgacggtccc ggccaaggtc gggcaagagc
      241 tgttcgagtc cgacagcctg aggactggtg atgacgggcg gatcggggtg accttcgagg
      301 acgacaccag gctgtcgctc gggcccgcga gcgaagtccg cctcgacacg ttccggtacg
      361 ctcccgcgga gggatccctc ggaatggcca tgcgttttct gcgcggcgta gccgtgtacg
      421 tctcgggcaa gatcgccaag ctcgcgcctg attccatccg tctcgaaaca ccatcggcca
      481 tcgtcggtgt tcgaggaacc acgctcgcca tcaacgtacg cgaatgacac gggcgtgggg
      541 gggcgtggtg ctcgtggcgg tgaccgcatg cctgaatgca gcgtgcggcc cgcagcggcc
      601 ccggaccccc caacgctcgg ggcaggacct ggtcgtcctg ctcccgaacg atgacggaac
      661 cactggcaaa gtcgaagtct ccaacccctc gggccgcgtc acgctggaca cggcccgcgc
      721 atcaacgctc atcgcggtcg gtcaggctcc aggagaggtc accgagctga ccgaggccga
      781 gattcagcgc atcttcgggc ccgcgctcgc cgcgcagccc ccggccccga ttcagttcac
      841 gctctacttc cagttcgact ccgaagagct gaccgacgac agccggcaga tgctctccga
      901 ggtgctgcag gtcgtgaaga aataccccgt tccccgcgtg accgtcgtcg gccacaccga
      961 caccaccggc tcggccgaat cgaatgtcga gctgggattg cgacgcgcca acgcggtccg
     1021 cgcgctgctc gtcgacgtcg gcgtcgaggg cgcggcgatc gacagtactt cccatggtga
     1081 ggcggcgctc ctggtcccca ccgcggacgg ggtcttcgag cccaagaacc ggcgcgtcga
     1141 cgtcacggtt cgatgagtcc ccggcatcgg cgaatcctcc tgctgtgcgg cttgctgccc
     1201 acgctgaccg ccgcgatctt gtccttgtac cgtcccgcgt ccttcgccgg cctcgagtac
     1261 gcggcctacg acacgctggt gcgatgggcc gacacgcgcc cgccgagcgg ccgcgtgctc
     1321 atcgtggatg tggacgagcg cagccttgca agcgtcggtc aatggccgtg gcggcgcgac
     1381 gtcgtcggcc gcctgatcga cggcctgaga agccgcggcg ccgccacggt tgcactcgac
     1441 atcatgttcc cggaagcgga tcgattcgaa gggtcgggtc ggtcccccga tgccgcgctg
     1501 gcgaagacgc tgcatcaggg acgcgtcatt ctcggttacg gtctcctctt cgcgtcgacg
     1561 gcgggggcgc caagcccgtg cctccggcac gcgctcggcc ttccgatcgt ccgcgggatc
     1621 ggcgcgccga cagacgatcc gttctttcga gccacgagcg cggtgtgcaa cataccggtc
     1681 ctgaccgacg cttccgatgc ctcagggttc ctgaatgccg ctccggaccc tgacggcatc
     1741 ctccgccgcg cgccgctgct catgcagtac gccgatcgaa tctacccggc actatcgctc
     1801 gcggccgtgc gtgccgtgac cgatgccaac gacctcctgc ttcgtgtcgc caacgtgaac
     1861 acctcctcgc tcacgcttgc cggccaggcc gtgccgctcg acggcaagag caatctgctg
     1921 ctgcgctacc gcggcccgaa gggaacgttc cgctacgtgt cggcggttga cgtcctgtat
     1981 ggcaagacgg acccggacgt gttcaaggac aagatcgtgt tcgtcggaac caccgcgctc
     2041 ggcacccgtg aggtcgtcgc tacgccgctc gacacgttgt tcgcaggcgt cgaagtgcag
     2101 gcgacgctgg ccgacaacct gcttcagcgc gacttcatat accgccccga gcacggcgtc
     2161 acgctcgaga cccaggccgt catcggcctc ggcgccatcg tcgccctcgt cgtcgcgcgt
     2221 ttcggcctcg tgtggggcgc gctcgcggcc gggagcttcg tggcctccat ctggatcggg
     2281 gcagactccc tgctggcgag cgacggcgcc tacttctcgc ccctctatcc gacgctggcg
     2341 ctcacgaccg gcctggctgc gatgacagtc gcggccttcg gcgtggagcg gcgtcgagcc
     2401 gagcgggccg ggcgcgaaaa ggagagctcg cggcggctga tggtgcagac gctgctgtcg
     2461 ttgacgggca tccgcgactt cgaaaccggg cgccactccc ttcgaacgca gaagtacacg
     2521 aaagtgattg ccgaggagct ctcgaagcat cccgatttcc gcgcctacct cacgccggag
     2581 cgcgtcgagc tcctggcgag cctcgcgccc ctccacgaca tcggcaaggt gggcgtgccg
     2641 gaccgcgtcc tgaacaagcc cggactgttg acgcccgacg agctcactga aatgcgccgt
     2701 catccccgct acggacgcga cgtcatcgag aacgccgagc gcctggccgg cgtccgcgac
     2761 gacgtggcgc tcgcggtggc gaaggacatc gtgtacacgc atcacgagtg gtgggacgga
     2821 tcgggctacc ccgaagggct gcgggggacg gccatcccga ttcccggccg tgtgatggcc
     2881 gtcgtggacg tatacgacgc gatcagaacc cgcaagctgt acca
//
LOCUS       DBNGSH010000920         2936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3176268, whole genome shotgun sequence.
ACCESSION   DBNGSH010000920 DBNGSH010000000
VERSION     DBNGSH010000920.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3176268"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1177)
                     /locus_tag="AC16CY_21045"
     CDS             complement(20..1177)
                     /locus_tag="AC16CY_21045"
                     /inference="COORDINATES: protein motif:HMM:NF017634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HIR3356353.1"
                     /translation="MHDAVVIGAGPAGLYAALRLAQNGRRVVLLEEHHVVGRPVHCTG
                     ILAREAFDQFDISADVILNELTAARFISPAGRDVVYRTRAIEAVAIDRPAFDTDLAER
                     AALAGVTFIHGARVTSVTRDARGVTVTAAGHPAVSARACVLACGGRYALHRHLGLSVP
                     SMYLHTAQKEVPAAHPGDVELHFGSHVAPKGFGWVVPIWREGRSYARIGVMSADRSPT
                     YFDRMVARVAGRWGVRPTEEGPPRQKILPLNRISRTYGDRLVVVGDAAGLVKPTTGGG
                     IYYSLVSASLAADVLTRAIDEDNLTSTRLAEYEQRWRERLEGELNTQLAFRSLAQSMR
                     DEDIEGLFELAQTDGVMPIVRRTATFNRHRRLIVALLKHPPARQLFFRSFL"
     gene            complement(1206..1964)
                     /locus_tag="AC16CY_21050"
     CDS             complement(1206..1964)
                     /locus_tag="AC16CY_21050"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3356354.1"
                     /translation="MYSMLREHALRAVTRRRGVSRVVNGLPFRVASPVRAAFAEDCDR
                     SVADFLRARVTPGSEVWNVGANVGVYALQLASWVGEAGRVVAFEPNPLAARFLRENLR
                     LNGLERRVEVVESAVADETGHVDLYVEGANGMARPGYPNPLLRHTTVVRTPVTTLDAF
                     LSRRPKAPSWIMMDIEGWEIAALRGARALLADTHVAVELHPSAWSWSGHSRADLEMVL
                     NEWDLVAVPLVGQSDALAQHGQVVLERRERSVDS"
     gene            complement(1964..2680)
                     /locus_tag="AC16CY_21055"
     CDS             complement(1964..2680)
                     /locus_tag="AC16CY_21055"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3356355.1"
                     /translation="MQRDSVALSGHRQGDILASPYRTRDRTLSVALAGRPQPAKRALD
                     LALSGVGLLASMPLWALVALLIKLEDGGPVFYGQERSGLNGVPFHVRKFRSMIQDAEA
                     RVGAVQAKERDPRVTRVGRLLRATAMDELPQLWNIFRGDMSFTGPRALRPGEIETLGS
                     GRVELLEDVPGFAERASVRPGLTGIAQIFAPRDIPRRLKFRYDLLYVRRQSFWLDVRL
                     IALSFWISFRGTWEARGGKF"
     gene            complement(2596..2910)
                     /locus_tag="AC16CY_21060"
     CDS             complement(2596..2910)
                     /locus_tag="AC16CY_21060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3356356.1"
                     /translation="MVQLEAMLCARPVVSTEVGTGVSWVNQHERTGLVVRPGDPRELH
                     HALARLVGDTVMRRRLGDAARARVLEHFTAETMCNATRLLYRDIGKATSSPARIEPAI
                     AR"
BASE COUNT          533 a         1005 c          980 g          418 t
ORIGIN      
        1 gaaatgactc ccgaccccat tacagaaatg atcggaagaa gagctgacgc gcggggggat
       61 gtttcagtaa cgccacaatc agacgccggt gccggttgaa cgtcgccgtg cgccggacga
      121 tgggcatgac gccgtcggtc tgggcgagct cgaacaggcc ttcgatgtcc tcgtcccgca
      181 tggactgcgc cagcgaccgg aacgcgagct gcgtgttgag ctccccttcc agccgctcgc
      241 gccagcgctg ctcgtattcg gccaggcgcg tagacgtgag attgtcctcg tcgatcgccc
      301 tggtcagcac atcggcagcc agcgacgcac tgaccaggct gtaatagatt ccaccgcccg
      361 ttgtcggctt cacgagaccc gccgcgtcac cgacgaccac gaggcgatct ccataggtac
      421 gggagatgcg gttgagcggg aggatcttct ggcggggcgg cccctcctcc gtcggccgga
      481 cgccccagcg cccggccact cgcgcgacca tgcgatcgaa atacgtcggc gaccgatccg
      541 ccgacatcac gccgatgcgc gcgtaggatc gaccttccct ccagatcgga acgacccagc
      601 caaaaccctt cggcgcgaca tgactgccaa aatgcagctc gacgtcgccc gggtgagccg
      661 ccggcacttc cttctgcgca gtgtgcaggt acatcgacgg gaccgacagg ccaagatgcc
      721 ggtggagcgc gtatcgccct ccgcacgcca gcacgcacgc gcgcgcagac acggcgggat
      781 ggccggcggc cgtcacagtc acaccccgcg catcgcgggt caccgatgtg accctcgcgc
      841 cgtggatgaa tgtgacgccg gccagagccg cacgctccgc caggtccgta tcgaaggcag
      901 gacggtcgat ggcgaccgcc tcgatggcgc gggtccgata gacgacgtcg cgcccggcag
      961 gcgagatgaa acgagccgca gtcagctcgt tgaggatgac gtcggcagag atgtcgaact
     1021 ggtcgaaggc ttcacgggcg aggatgccgg tacagtggac cgggcggccc acgacgtgat
     1081 gctcctcgag aagtacgacg cgccgtccgt tctgcgccaa tcgaagggct gcatacaagc
     1141 cagccggacc agcgccaatg acgaccgcat cgtgcattca ggctccgagt ttccacatcg
     1201 gcagctcagc tgtcaacgct gcgctcgcga cgttccagga cgacctggcc gtgctgagcg
     1261 agggcatcgg actgaccaac caacggtacg gctaccaggt cccactcatt gagcaccatt
     1321 tcgaggtccg cgcgcgagtg acccgaccac gaccacgcag aagggtgcaa ctcgacggcc
     1381 acgtgcgtgt cggcgagaag ggcgcgagcc ccgcgcagcg cggcgatctc ccagccctcg
     1441 atgtccatca tgatccacga cggcgccttc gggcggcgcg agagaaacgc gtccagcgtc
     1501 gtcaccggtg tgcgaacgac ggtcgtgtgt cgaagaagtg gattggggta ccccggacga
     1561 gccatgccgt tggcgccctc gacatagaga tccacgtgcc ccgtctcgtc ggccacggcg
     1621 gactcgacga cctccacgcg ccgctcgaga ccgttgagtc gcaggttctc acgcaggaat
     1681 cgggccgcca gtggattggg ctcgaaggcc acgactcgcc ccgcctcgcc tacccaggag
     1741 gcgagctgca gggcgtacac gccgacgttg gcgccaacgt tccacacctc ggagccgggc
     1801 gtgacgcggg cgcgcaggaa atcagccacg gagcgatcgc agtcctcggc aaaggcggcg
     1861 cggacgggcg aggcgacgcg gaacgggaga ccgttcacca cccgcgatac gccacggcga
     1921 cgcgtgaccg cccggagtgc gtgctctcga agcatcgagt acatcagaac ttgccgcctc
     1981 tcgcttccca cgttccgcgg aaggagatcc agaaggacag cgcgatgagg cgcacgtcga
     2041 gccagaagga ctgccggcgg acgtacagaa ggtcgtagcg gaatttcaaa cggcgcggga
     2101 tgtcgcgcgg cgcgaagatc tgggcgatgc ccgtcagacc gggccggacg ctggcgcgct
     2161 cggcgaagcc cggcacgtct tccagcagct ccacccgccc ggagcccagc gtctcgatct
     2221 caccgggcct gagcgcccgc ggacccgtga agctcatgtc gccgcgaaag atgttccaga
     2281 gctgcgggag ctcgtccatc gccgtcgccc gcaggagccg gcccacgcgt gtcacgcgcg
     2341 gatcgcgctc tttcgcctgg acggcgccca cacgggcctc ggcgtcctgg atcatggagc
     2401 ggaacttccg cacgtgaaag ggcacaccat tgaggccaga ccgctcctgc ccgtagaaca
     2461 ccgggccgcc gtcctccagc ttgatgagga gcgcgacaag cgcccagagc ggcatcgacg
     2521 cgagcaggcc aacgccggag agcgcgaggt cgagcgcccg cttggcgggc tgcggccgtc
     2581 ccgccagcgc gacgctcagc gtgcgatcgc gggttcgata cgggctggcg aggatgtcgc
     2641 cttgccgatg tcccgataga gcaaccgagt cgcgttgcac attgtctccg ctgtgaagtg
     2701 ttcgagcacg cgcgcgcgcg cggcgtcacc cagtctgcgt ctcatgacgg tgtcgccgac
     2761 cagacgggcc agagcgtggt ggagctcgcg cgggtcgcct gggcgcacga caaggccggt
     2821 gcgctcgtgt tgattgaccc aggagacgcc cgtcccgacc tccgtgctga cgaccgggcg
     2881 cgcgcagagc atggcctcga gctgcaccat cccgaaggct tcctggcgcg tgatcg
//
LOCUS       DBNGSH010000921         6193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1817212, whole genome shotgun sequence.
ACCESSION   DBNGSH010000921 DBNGSH010000000
VERSION     DBNGSH010000921.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1817212"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="AC16CY_21065"
     CDS             complement(<1..534)
                     /locus_tag="AC16CY_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356357.1"
                     /translation="MSRLSLAALIALATIALQAQDQPTFRAGANFIHVDMYASADGKS
                     VEDLTRDEVEILEDGVRQTIDAFEYVKVRPATQEERREPDTLEQSRQGAADPRARVFV
                     IFLDTYHTTIEGSANMRLPLIKFLDRLLGPDDLVALMTPEMDASSIALGRKTTVISNI
                     MQAEWMWGRRGRTAGDKD"
     gene            complement(566..1276)
                     /locus_tag="AC16CY_21070"
     CDS             complement(566..1276)
                     /locus_tag="AC16CY_21070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3356358.1"
                     /translation="MLIAVRSVVAYIAILSYIAVAGPLALVLGGLLRWTNGVFILGHW
                     GVWLALALAGIRYRVAGRAPDSTTAVVFCSNHESNVDPAVLFKVLHPRLRVLYKAELH
                     KFPVMGTLFDIGGFVPVDRADREKSAASITRGAASLKAGRSFLIFPEGTRSRTGELLP
                     FKKGGFIMAIEAQVPIVPVAVTGGRDAMRKGSAFVRPVTVSVRIGQALPTEGLTLDDR
                     DALIERVRGEIGALRQLG"
     gene            complement(1385..1981)
                     /locus_tag="AC16CY_21075"
     CDS             complement(1385..1981)
                     /locus_tag="AC16CY_21075"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR3356359.1"
                     /translation="MTFQLGDKVIYPNHGLGIVERIEEKTILGTTCGFFHLRIVSNDT
                     TVLVPVANVDNVGLRKAITDDEVDRLFNLLSDGRIDNHQNWKGRFKDNSDKMRTGSIY
                     DMADVLKSLTFLAQSKSLSFREKRMLDRAKALIISEVSEVMREKAPEVETKVDRALER
                     CFMVKARNVARATARTKAKPVKAPMVMAAAPRRAARAS"
     gene            2241..2804
                     /locus_tag="AC16CY_21080"
     CDS             2241..2804
                     /locus_tag="AC16CY_21080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3356360.1"
                     /translation="MGLRLPGLVGVSLIGSLCMAQLAPGQSRSLAPHVATPPEVVERM
                     LKLAKVGPSDLVYDLGCGDGRIVIAAAQKFGARGVGVDIDAGLIRQAEVNAKAAGVSP
                     RVRFRVQDAMTVDVSDATVVTLYLLSASNVKLRPILTRQLHKGSRIVSHSFAMGDWEP
                     DVVDTFTDQGGTTRTLYLWQVDGKPRP"
     gene            complement(2801..3823)
                     /locus_tag="AC16CY_21085"
     CDS             complement(2801..3823)
                     /locus_tag="AC16CY_21085"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3356361.1"
                     /translation="MSKLKIVVLYDRVLVDESDDQAASPDKAPVTRSLDKKEVEEEVA
                     EALEKLGHEPTLHELDGTPKSLLALAKLECDLVFNLTESFAGDDTADFKIAGFLELIG
                     KKYTGSGTHGLMLAQDKAIAKKIFAFHGIHTPVFAKSFRGRLDFSHDLQFPVIVKPAR
                     EDGSIGIEFSAVVSSIRELMERMDWLHANFDSPVLIEEYIEGREMYVGVIGNDKPAAL
                     PVVELDLSKLPDGTPRIASAEVKWGKGTKAYRDTKSAVATDLPEENVLALQQTAVAAY
                     QALELRDYGRVDMRLQADGRVHVIEVNPNPWLSSKAEFAMAARKAGKTYPQLVEEIVE
                     LAMARA"
     gene            3854..5092
                     /locus_tag="AC16CY_21090"
     CDS             3854..5092
                     /locus_tag="AC16CY_21090"
                     /inference="COORDINATES: protein motif:HMM:NF018434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3356362.1"
                     /translation="MRFVMTPVGSAGDVHPFVAIGRALRSRGHEVVVATAEPFGNAVR
                     RAGLGFHQTISADEFDAAVKNPDLWHPYRGLRVVLGEVASRLRPGYEHVKALYEPGRT
                     VLVGHSISFFTRVFEELHGAPAATLHLAPSVFRSDYQQPAYAPGLDGSTWPRWIKRSA
                     WWTIDRLMVDPPIAPALNAWRRDMGLPPVDRPFRDWIHSPRRVVGLFPDWFGQPQPDW
                     PKALRLTGFPLYDESDHAEVPMGTSRFMDAGSPPIVFTPGSANQQAAGFFRAAVDASA
                     RLGRRALLLTRYPAQLPPLPPFAHHESFVPLSKVLPRCAALVHHGGIGTLAQGLAAGI
                     PQLTMPMGFDQPDNATRLHRLGVGRWIRPSKFTGQPVADALHELIEDPHTRMQCRHWA
                     NRMGTGAALDQTCRLLEELA"
     gene            5103..>6193
                     /locus_tag="AC16CY_21095"
     CDS             5103..>6193
                     /locus_tag="AC16CY_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3356363.1"
                     /translation="MTADYEKLGLFYLGRRYDLSAGARLPDPVLYDSKDLLTHAVCVG
                     MTGSGKTGLGLGLIEEAAIDGIPVVAIDPKGDLPNLLLTFPNLLPSDFAPWVDPAEAV
                     RRNVSVEAHAAEIAQRWKDGLTDWGQDGGRISRLRAAADIRVYTPGSRASTPLAVLGS
                     FGHAHHETGEDTQARIAATAAALLGLVGITDVQPHSREQALLSAILASQPAGDAGNLA
                     WIVQQIQRPSFERVGVLDLETFYPARERQDLALRFNSVLASPGFDLWSTGEPLDIGSL
                     LFTREGRPRVAIVSIAHLDESQRMLVVSLVLSAVLQWTRRQSGTGSLRALVYMDEVFG
                     YLPPVANPPSKAPLLTLLKQARAYGVGIVL"
BASE COUNT         1060 a         2036 c         1966 g         1131 t
ORIGIN      
        1 gtccttgtct ccagccgtgc ggccccgccg gccccacatc cactcggcct gcatgatgtt
       61 ggaaatgaca gtcgtcttac ggccgagcgc gattgagctg gcgtccatct ccggcgtcat
      121 caacgccacc aggtcgtcgg ggccgaggag ccgatccagg aacttgatga gcggcagccg
      181 catgtttgcg gagccttcta tcgtggtgtg gtaggtgtcc aggaagatga cgaacacgcg
      241 cgcacgggga tcggccgccc cctggcggct ttgttcgagc gtgtccggct ccctgcgttc
      301 ctcctgcgtg gccgggcgga ccttcacgta ctcgaacgcg tcgatggtct gacgcactcc
      361 gtcttcgaga atctcgactt cgtcgcgggt caggtcctcg acactcttcc cgtcggcgga
      421 ggcgtacatg tcgacgtgga tgaagttggc gccggcgcga aatgtcggct ggtcctgcgc
      481 ttgcagcgcg atcgtcgcga gcgcgatgag ggcggcaagt gaaagtctag acacgggcac
      541 ccgcgagcag gctagcaagg caagtctacc ctaactgccg gagcgccccg atttctccac
      601 ggacccgctc gatcaaggcg tcgcggtcgt caagggtcag gccctcggtc ggcagagcct
      661 gtcctattcg gacgctcacc gtgacgggcc gcacgaaggc actccccttt ctcatggcgt
      721 cccggccccc cgtaaccgca acgggcacga tgggaacctg tgcctcgatg gccatgatga
      781 atcctccctt cttgaacggg agcagctcac ccgtccggct ccgagtcccc tccggaaaaa
      841 tcaggaacga gcggcccgcc ttcagtgagg cagccccgcg cgtgatcgat gccgccgact
      901 tttcgcgatc ggcgcgatcg actggcacga accccccgat gtcgaacaac gtccccatca
      961 ccgggaattt gtggagttcg gccttgtaga gcacgcggag acggggatgc aggaccttga
     1021 agagcacggc tggatcgacg ttgctttcat gattcgagca gaacacgacg gcggtggtcg
     1081 aatcgggcgc ccggccggcc accctgtagc ggatccctgc gagtgccagg gcgagccaca
     1141 cgccccaatg ccccaggatg aacacgccgt tggtccaccg cagcagacct cccagtacga
     1201 gcgccagcgg ccccgccacg gcgatgtagc tcagaatggc gatgtaggcg acaacggaac
     1261 gaacggcgat gagcatcctc tgtgggctac aggctacagg ctatcggcta ccctctagag
     1321 ttccagtaca aaaaaacgcc cggaccatcg gtccgggcgt caaagaagca accgggaaga
     1381 gcgactagga cgctcgagcc gcgcggcgcg gagcagccgc catgaccatc ggcgccttca
     1441 ccggcttcgc cttggtcctg gctgtggcgc gcgccacgtt gcgtgccttg accatgaagc
     1501 agcgttcgag cgcgcggtca actttggtct cgacctccgg cgccttctcg cgcatcacct
     1561 cggacacctc cgagatgatc agcgccttgg cgcgatcgag catccgcttc tcacgaaacg
     1621 agaggctctt cgactgcgcc aggaaagtca ggctcttcag gacgtcggcc atgtcataga
     1681 tcgacccggt ccgcatcttg tcggagttgt ccttgaaccg tcccttccag ttctggtgat
     1741 tgtcgatccg gccgtcgctg agaaggttga acagacgatc gacttcgtcg tcggtgatcg
     1801 ccttgcgcag tcctacgttg tcgacgttcg caaccggcac cagcacggtc gtgtcgttgg
     1861 aaacgatccg cagatgaaag aacccacacg tggtacccaa aatagtcttt tcttcgatcc
     1921 gttcgacgat gccgagtcca tggttcgggt agatgacctt gtcacctagc tgaaatgtca
     1981 cgttgccccc gttgcaggaa ggttggtcct agcggccgat gctgggaaat gcagtgatga
     2041 gtatagctca ctcgccgggg cggatcaacg gaaaatgccc aaaaaaggcc cggtttaggc
     2101 acttcaggcc gcgcaattgc ccgttgggcg gggcggcccg ggatttgtcc gaggtcgccc
     2161 ggttaactcg ctcgtagcgc aggacttgag tcgaagcctc gaccgggacc tcgcagggcc
     2221 ctgaagtaga atccctcgcc atgggcctgc gacttccagg tctcgtgggc gtcagcctca
     2281 tcggcagcct gtgtatggct cagttggcgc ccggccagag ccgcagtctc gccccccacg
     2341 tggccacgcc gcccgaggtc gtcgagcgca tgctgaagct ggccaaggtg gggccctcag
     2401 acctggtcta cgacctgggg tgcggcgatg gccgcatcgt catcgcggcg gcccagaaat
     2461 tcggggccag gggggtgggg gtggacatcg acgccggcct gatcaggcag gcggaagtta
     2521 atgcaaaagc ggccggcgtc tcgccccgcg tcaggttccg ggtccaggac gcgatgaccg
     2581 ttgacgtgtc ggacgccacg gtcgtcacgc tttatctcct gtcggcgtca aacgtgaagc
     2641 tgcggcccat cctcacccgt cagcttcaca agggctcgcg aatcgtctcc cacagcttcg
     2701 cgatggggga ttgggagccc gacgtcgtgg acacgttcac ggaccagggc ggcaccacgc
     2761 ggacgctgta tctgtggcag gttgacggga agccgagacc ctaggccctg gccattgcga
     2821 gctcgacaat ctcctcgacc agctgcggat aggtcttgcc ggccttcctg gccgccatcg
     2881 cgaactcggc cttcgagctg agccaggggt tcgggttgac ctcgatcacg tgtacccggc
     2941 catcagcctg cagccgcatg tccacgcggc cgtaatcccg cagctccaac gcctgatagg
     3001 cggcgacggc ggtctgctga agcgcgagca cgttctcttc gggaaggtcc gttgccacgg
     3061 ccgacttcgt gtcgcggtag gccttagtgc ccttgcccca tttcacttcc gccgacgcga
     3121 tgcgaggcgt gccgtcggga agcttggaca aatcgagctc cacgaccggc agcgccgcgg
     3181 gcttgtcgtt gccgatcacg ccgacataca tttcccgccc ttcgatgtac tcctcgatca
     3241 acactggcga atcgaaattg gcgtgcagcc agtccatgcg ctccatcagc tcacggatcg
     3301 agctgacgac cgcgctgaat tcgatgccga tcgagccgtc ctcgcgggca ggcttgacga
     3361 tgacgggaaa ctgcaggtcg tgcgagaagt cgaggcggcc gcggaacgac ttggcgaaca
     3421 cgggtgtgtg gatgccatga aaggcgaaga tcttcttcgc aatcgccttg tcttgcgcga
     3481 gcatcaggcc atgagtgccg gaacccgtgt atttctttcc aatgagctcc aggaatcccg
     3541 caatcttgaa atcggccgtg tcatcgccag caaacgactc cgtcaggtta aagaccagat
     3601 cgcattccag cttcgcaagc gcgagcaggc ttttcggcgt tccatcgagt tcgtgcaacg
     3661 tcggttcgtg accgagcttc tcgagcgctt cagcgacctc ttcctcgact tcctttttgt
     3721 ccagactccg cgtgacgggg gccttgtcgg gggaggcggc ctgatcgtcg gattcgtcca
     3781 cgagcacacg gtcgtacagg acgacaatct tgagtttgct catcgatgat cgagctaact
     3841 ttacacttct accatgcgtt tcgtcatgac gccggtcgga tccgccggcg atgtccatcc
     3901 attcgtcgca atcggacggg cgctgcgctc gcgcggccac gaggtcgtcg tcgcgacggc
     3961 ggagccgttc ggcaacgccg tccggagggc tggcctgggg ttccatcaga ccatctccgc
     4021 cgacgaattc gacgcggcgg tgaagaaccc cgacctctgg catccgtatc gcggactgcg
     4081 agtcgtcctc ggtgaggttg catccaggct gcggcccggt tacgagcacg tgaaggccct
     4141 gtacgagccg ggacggacgg tgctcgtcgg ccactcgatc agcttcttca cccgcgtgtt
     4201 cgaagaactg cacggcgcgc ccgcggcaac gctgcatctg gcgccatcgg tcttcaggtc
     4261 cgactaccag cagcctgcat acgcaccggg cctggacggc tcgacgtggc ctcgctggat
     4321 caagcgcagc gcgtggtgga cgatcgaccg attgatggtg gatccgccga ttgctcccgc
     4381 gttgaacgca tggagacgcg acatgggact gcctcccgta gaccgtcctt ttcgtgactg
     4441 gatacattcg ccgcgacggg tggtcggcct ctttccggac tggttcgggc agccccagcc
     4501 cgactggccg aaggcccttc gtctgaccgg ctttccgctg tacgacgaat cggaccacgc
     4561 ggaggtcccg atgggaacgt cgcgtttcat ggacgccggc tctcctccga tcgtcttcac
     4621 tcccggctcg gcgaaccaac aggccgcggg gttcttcagg gccgccgtcg acgcgagcgc
     4681 gcgcctcgga cgccgggcgc tgctgctcac gcgatacccg gcccagcttc cgccgctgcc
     4741 gccgttcgcc catcacgagt ccttcgtccc gctttcgaag gtgcttccga ggtgcgccgc
     4801 gctcgttcac cacgggggca tcggcacgct cgcccagggt cttgcggccg gcattccgca
     4861 gttgacgatg ccgatggggt tcgatcagcc ggacaacgcc acgcggcttc accggcttgg
     4921 cgtgggacgc tggattcgac cgtcgaagtt caccgggcag cctgtcgccg acgcgcttca
     4981 cgagctgatc gaagacccgc acaccaggat gcagtgccgg cattgggcga atcggatggg
     5041 cacgggcgcg gcgctcgacc agacatgccg tctgctcgag gagcttgcat aatggatggc
     5101 tcatgacggc cgactacgag aaactggggc tgttctacct cggacggcgg tacgacctgt
     5161 cagcgggcgc ccgcctgccg gatcccgttc tctacgactc gaaagacctg ctcacgcacg
     5221 ccgtctgcgt cgggatgacg ggcagcggca agaccggtct cgggctcggc ctcatcgagg
     5281 aggcggccat cgacggcatc cctgtcgtcg cgatcgatcc caaaggcgac ctgcccaatc
     5341 tgctcctcac gtttccgaat ctgctgccgt cggatttcgc gccctgggta gatccagcag
     5401 aagccgtgcg gcggaacgtg agcgtcgagg cgcatgcggc cgagatcgcg cagcgctgga
     5461 aggacggact caccgactgg ggccaggacg gcggccgcat cagccggctt cgcgccgccg
     5521 ccgacatacg cgtgtataca cccggaagcc gcgcgagcac gccgctggcc gttctgggat
     5581 cgttcggaca tgcgcaccac gagacaggag aagacacgca ggctcggatt gcagccactg
     5641 ccgcggctct cctcggcctg gtcgggataa cggacgtcca gccccacagc cgggagcagg
     5701 cgctgctctc cgcgatcctg gcgagccagc cagccggcga cgcgggcaac ctcgcgtgga
     5761 tcgtccagca gatccagcgg ccttccttcg agcgcgttgg cgtgctggac ctcgagacgt
     5821 tctatcccgc gcgggagcgg caggatctgg cactccggtt caacagcgtc ctggcgtctc
     5881 ccggattcga tctctggagc acgggcgaac cgctcgacat cgggtcgctg ctcttcacgc
     5941 gcgaaggccg cccgcgtgtg gcaatcgtgt cgatcgcgca cctggacgag tcgcaacgga
     6001 tgctggtcgt gtcgctcgtg ctgagcgccg tgctgcagtg gacccggcgc cagagcggga
     6061 ccggatcgct gcgggcgctg gtctacatgg acgaagtgtt cggctacctg ccgcccgtgg
     6121 ccaacccgcc gtcgaaggcg ccgcttctca cgctcctcaa acaggctcgc gcctacggcg
     6181 tcggcatcgt act
//
LOCUS       DBNGSH010000922         5792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4705294, whole genome shotgun sequence.
ACCESSION   DBNGSH010000922 DBNGSH010000000
VERSION     DBNGSH010000922.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4705294"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="AC16CY_21100"
     CDS             complement(<1..195)
                     /locus_tag="AC16CY_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3356364.1"
                     /translation="MRIGIDVGGTKIEGIALDSTREAARIRVDTPRDDYAATLNAIVA
                     VVADLERQTGARGTVGVGIPG"
     gene            245..427
                     /locus_tag="AC16CY_21105"
     CDS             245..427
                     /locus_tag="AC16CY_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356365.1"
                     /translation="MRSYLWTSFVALSVVTALTQTPLSGRPFDAQFDADIRGFFSDFY
                     HVELTDQQLRRLVAAP"
     gene            complement(452..3949)
                     /locus_tag="AC16CY_21110"
     CDS             complement(452..3949)
                     /locus_tag="AC16CY_21110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3356366.1"
                     /translation="MYIELHAASAFSFLQGASLPESLIERAAALDYPALALLDADGVY
                     GAPRFHKAAKQAGLRPIVGAELTIQASGLGPQASGLGKGKGLRPGQALRPEAWALRPA
                     RWRLPVLVASSEGYKNLCRLVTRMKLRAPKGGVPAAHACAVGCKGEGALALEELDGFT
                     AGLVALAGRPVLDVRQHGVGGLLDRLVGLFGRANVCVELQRHHMRDEESDIAALTELA
                     GAYRVPTIATNDVRMATAADRPLFDVLTCVRHHTTLDEAGRRLARNAERYLKSPEVMA
                     ELFADRKDALKGTVALAERLEYTMADLGYRFPAYPVPSGETAASFLRHVAEAGARDRY
                     RPYHDKARAQIARELDLIEKLDLAGYFLIVWDIVNYCRQQNILVQGRGSAANSAVCYS
                     LGITAVDPIRMELLFERFLSEERGEWPDIDLDLPSGDRRERVIQHVYEKYGRLGAAMT
                     ANVITYRSRSAAREVGKALSLDEGRIDRLAKVMHNFEFADPHETLARNMEAAGLSLDM
                     PRVRAFADLWTAIQDLPRHLGQHSGGMVVSQGALDSVVPLEPASMPDRVVVQWDKDDC
                     ADMGIVKVDLLGLGMMAVLQDAIEVVNTEGGRGQGAGDRAVSGLSRIDLAHLPPDDPA
                     VYDMLQKADTVGVFQVESRAQMATLPRLKPTCFYDLVVEVAIIRPGPIVGQMVHPYLN
                     RRAGREPVVPLHPSLEPVLARTLGVPLFQEQLLRMAMVAAGFTGGQAEELRRAMGFKR
                     SEKRMKQIEVQLREGMSRNGITGETAERIVTSITSFALYGFPESHAASFALLAYASAY
                     LKAHYSSAFYAALLNNQPMGFYHPATLVKDAQRRGVRFHPIDVQQSDWLCRVEEDGGI
                     RIGFMYVNGLRKDAGQAMAAVDRTKTIAPAPLCPKCGADDESMIEIVREGPRPQSALS
                     PAPCPLPPSRSYYCNICSHEWTVPLQPVGRFRSIEDVVQRTGLRRDELTTLADIGALN
                     AFTNERRSALWQVERSVRPAGELFGGQEINSQLPPSNSQEPKAKSPEPGAQSPLPVMD
                     AAERLQADYAGMGLTIGPHPMALRRSELALRGVLRASDLPRARDGRRVRVAGMVITRQ
                     RPGTAKGFVFLTLEDETGISNIIIRPDLYDRDRTTVVRQPFLMVEGVLQHQDGVISVR
                     AERVEAVQGDAAVDAHDFY"
     gene            complement(3989..5467)
                     /locus_tag="AC16CY_21115"
     CDS             complement(3989..5467)
                     /locus_tag="AC16CY_21115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR3356367.1"
                     /translation="MFGALHGPEGTPPGVLADVAREFSPRIEIDTPRDVLLDLSGLTR
                     LFGDARTIAEHMRRTAADRGVRVRVAIAGTRTAARLLVRHRAGLTVVEPGTEAATLAA
                     LPIALLTAIHPDAVNSQLPPLDHARGGLSDSRRPTPNSQDGRPPRRHRGTEKNVRDPI
                     EQLSTLRRWGLRTLGDLAALPSDEVASRLGHDGTEWQRLARGEDPRPLVPAVPEERFE
                     QSLELEWPIEEIEPLSFVLGRLMEPLSAHLEQRDRGAAVLHVRLDLVKTAGVARDVFE
                     RSLQLPFPIRDARTLRTLALLDLESHPPPAAIDRVTVAVDPTPARIVQWSLLSRALPS
                     PEHISTLMARLQALMGETRCGSPVVVDSWQPGVFAIKPFAPSDASLSHDGRHVPSMPL
                     WLTAVPSSPMPVVALRRFRHPVPVRVQAEHGRPSSLMSMRRGLSGGRVESCAGPWRTS
                     GGWWEPEPWDRDEWDVALTGGVTYRLLRERNTNRWLLEGVFD"
     gene            complement(5472..>5792)
                     /locus_tag="AC16CY_21120"
     CDS             complement(5472..>5792)
                     /locus_tag="AC16CY_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356368.1"
                     /translation="LQAGGFGIVAIDLADAPPRELRQIPFTTWPRVQRAIEGRDTACV
                     LLTEEPLARSAGGLTLSLAGRSTWAGDSIRSRRLLGADVSVRVVSPRKRIDGNVVITT
                     RCSH"
BASE COUNT          945 a         2024 c         1912 g          911 t
ORIGIN      
        1 gcccggaatg ccgacgccga ctgttccacg agcaccggtc tgccgttcga gatcggcgac
       61 gaccgcgacg atcgcgttca gcgtggctgc gtagtcgtcc cggggtgtgt caacgcggat
      121 gcgggccgcc tcgcgcgtgc tgtcgagcgc gatcccttcg atcttggtac cgcccacatc
      181 gatgccgatg cgcacggggg cagcttagct caaggccgtg ccacgaatgc ggtagcctac
      241 tcgaatgcgc tcgtacctct ggacgtcgtt cgtcgctctc tcggtcgtga cagccctgac
      301 gcagacgccg ctttcagggc gcccgttcga cgcgcagttc gacgccgaca tccgcggttt
      361 cttcagcgac ttctaccacg tggagctcac cgatcagcag cttcgaaggc tcgtcgccgc
      421 cccctaatcc cttccccctt cccccctccc cctaataaaa gtcgtgcgcg tccacagcgg
      481 catctccctg gaccgcctcg acccgctcgg cgcgaaccga gatcacgccg tcctggtgct
      541 gcaacacgcc ttcgaccatc aggaatggct gccggaccac cgtcgtccgg tcacgatcgt
      601 agagatcggg ccggatgatg atgttcgaga tgcccgtctc gtcctcgagc gtgaggaaga
      661 cgaagccctt ggccgtgccc ggccgctggc gtgtgatgac catgccggcc acccgcacgc
      721 ggcggccgtc gcgcgcgcgc ggcagatcgg aggcccgaag cacgccgcgc agcgccagct
      781 cgctgcggcg cagcgccatc gggtgcggac cgatcgtcaa ccccatcccg gcgtaatcgg
      841 cttgcaggcg ctcggcggca tccatgactg gaagcggact ctgggcgccc ggctctgggc
      901 tcttggcttt cggctcctgg gagttggaag gtggaagttg ggagttgatc tcctgtccac
      961 cgaatagttc gcctgccggc cgcaccgatc gctccacctg ccagagcgcg ctccgccgct
     1021 cgttggtgaa cgcattgagc gcgccgatgt cggccagcgt cgtcagctcg tcgcgacgca
     1081 aaccggtccg ctggacgacg tcttcgatcg aacggaagcg cccgaccggc tggagcggaa
     1141 ccgtccactc gtgcgagcag atgttgcagt agtaagaacg tgaagggggc agggggcagg
     1201 gggcagggga cagggctgat tgaggcctgg ggccttcacg gacaatctct atcatcgact
     1261 cgtcgtcggc gccgcatttg ggacacaacg gcgcgggcgc aatcgttttc gtcctgtcga
     1321 cagcagccat cgcctgacct gcgtcctttc gcaaaccgtt cacgtacatg aagccgatgc
     1381 ggatcccgcc gtcctcctcg acgcggcaga gccagtcgga ctgctgtacg tcgatcggat
     1441 ggaagcggac gccgcggcgc tgcgcgtcct tgacgagcgt cgcagggtga tagaagccca
     1501 tcggctggtt gttgaggagc gccgcgtaga acgcggatga gtagtgcgct ttcaggtacg
     1561 cgctcgcata cgccagcagc gcgaagctgg ccgcgtgcga ttcggggaat ccatagagcg
     1621 caaacgaggt gatcgaggtg actatccgct ccgctgtctc gccggtgatg ccgttgcgcg
     1681 acattccctc gcggagctgc acctcgatct gcttcatccg cttctccgag cgcttgaagc
     1741 ccatcgcgcg ccgcagctcc tctgcctgcc cgccggtgaa tccggcggcc accatcgcca
     1801 tccgcagcaa ctgctcctgg aagagcggca cgcccagcgt gcgcgccagc accggctcga
     1861 gcgacgggtg cagcgggacg accggctccc gtcccgcccg gcggttgaga taggggtgca
     1921 ccatctgccc gacgatcggg ccgggacgaa tgatcgccac ctccacgacc aggtcgtaga
     1981 agcaggtcgg cttcagccgc gggagcgtcg ccatctgcgc gcgcgactcg acctggaaga
     2041 cgccgacggt gtcggccttc tgcaacatgt cgtagacagc gggatcatcc ggcggtaaat
     2101 gagcgagatc gatccgactc aatcctgaga cagccctgtc ccctgccccc tgccccctgc
     2161 ccccttcggt gttcaccacc tcgatggcgt cctgcagcac cgccatcatg ccgaggccga
     2221 gcagatcgac cttgacgatg cccatgtcgg cgcagtcgtc cttgtcccac tggacgacca
     2281 cgcggtccgg catgctcgcg ggctcgagcg gcaccaccga atcgagcgct ccctgcgaca
     2341 ccaccatccc gccggaatgc tggccgagat gccggggcag gtcctggatg gccgtccaca
     2401 ggtcggcgaa cgcccgcacg cgcggcatgt cgagcgaaag acccgcggcc tccatgttgc
     2461 gcgcgagcgt ctcgtgcggg tcggcgaact cgaagttgtg catgaccttg gcgagccgat
     2521 cgatccggcc ctcgtcgagc gacaacgcct tgcccacttc gcgcgccgcg ctgcggctgc
     2581 gataggtgat gacgttggcc gtcatggccg cgccgagccg gccgtatttc tcgtacacgt
     2641 gctggatgac gcgctcgcgc cgatcgccgc tcggcaaatc gagatcgatg tcgggccatt
     2701 ccccgcgctc ctcagacagg aagcgctcga acagcagctc catcctgatg ggatcgaccg
     2761 cggtgatccc gaggctgtag cagacggcgc tgttggccgc cgacccgcgg ccctgcacga
     2821 ggatgttctg ctggcggcag tagttgacga tgtcccagac gatcaggaag tagccggcga
     2881 gatcgagctt ctcgatgagg tcgagctcgc gggcgatctg cgcacgcgcc ttgtcgtgat
     2941 agggccggta ccggtcgcgc gcgccggcct cggcgacgtg gcggaggaac gacgccgccg
     3001 tttcgccgga cggcaccgga tacgccggaa accgataccc cagatcggcc atcgtgtact
     3061 cgagccgctc ggccagcgcc acggttccct tcagcgcatc cttgcgatcg gcgaacagct
     3121 cggccatcac ctcgggcgac ttgaggtacc gctcggcatt acgcgccagg cggcgccccg
     3181 cctcgtcgag cgtcgtgtga tggcgcacgc acgtgagcac gtcgaacagc gggcggtcgg
     3241 cggccgtggc catgcgcacg tcgttggtgg cgatcgtcgg gacgcggtag gcgcccgcca
     3301 gctcggtgag cgccgcgatg tccgattcct cgtcacgcat gtggtgacgc tgcagctcca
     3361 cgcacacgtt ggcccgcccg aagaggccga cgagccgatc gagcagcccg cctacgccgt
     3421 gctgccgcac gtcgagcacg ggccggccgg ccagcgccac gaggccggcc gtgaagccgt
     3481 cgagctcctc gagggcgagc gctccctcgc ccttgcaccc caccgcgcaa gcgtgcgcgg
     3541 cggggacccc gcccttcggc gcacgcagct tcatgcgggt cacgaggcgg cataaatttt
     3601 tgtaaccctc tgacgatgcc accagtacag gaagccgcca tcgagcaggc ctcagggccc
     3661 aggcctcagg cctcagggct tgtccaggcc tcaggccttt tcccttgccc agccctgagg
     3721 cctgaggccc gaggcctgag gcctgaatgg tgagttcggc accgacgatc gggcggagcc
     3781 ccgcctgctt cgcggccttg tgaaagcgcg gcgccccgta gacgccgtca gcatcgagga
     3841 gcgcaagcgc gggatagtcg agcgccgccg cgcgctcgat gagcgactcg ggcagcgaag
     3901 caccctggag gaagctgaac gcggacgcgg catggagctc gatgtacata aaggcctcag
     3961 ggcccaggcc tcaggcctca gccttctgtt aatcgaacac cccctccagc aaccatcggt
     4021 tcgtgttgcg ctcgcggagc agccgatagg tgacaccgcc cgtgagcgcc acgtcccatt
     4081 cgtcgcgatc ccagggctcg ggttcccacc agcctcccga tgtccgccac ggccccgcgc
     4141 acgattccac tctcccgccg ctcaacccgc gccgcatcga catgagactc gagggtcgtc
     4201 cgtgctccgc ctgcacgcgg accggaacgg ggtgacggaa gcgccgaagc gcgacgaccg
     4261 gcatgggcga ggacggcaca gccgttaacc acagaggcat agagggcaca tgacgtccgt
     4321 cgtgggacag cgatgcatcc gaaggcgcga agggcttgat cgcgaacacg cccggctgcc
     4381 acgagtcgac caccactggc gatccgcagc gcgtctcgcc catgagtgcc tgaaggcggg
     4441 ccatcagcgt ggatatgtgt tccggcgacg gcagcgcgcg cgacagcagc gaccactgca
     4501 cgatgcgggc gggtgtggga tccacggcca ccgtcacccg atcgatcgcg gcgggaggcg
     4561 gatgcgactc gagatcgagc agcgcaagcg tgcgcagcgt gcgcgcgtcg cggatcggga
     4621 acggcagctg caacgatcgc tcgaagacgt cgcgcgcgac gccggcggtc ttgacgagat
     4681 cgagacgcac atggaggacg gccgcccccc tgtcgcgctg ctcgagatgg gccgagagcg
     4741 gctccatcag ccgtcccagc acgaacgaca acggctcgat ttcctcgatc ggccactcga
     4801 gctcgagcga ctgctcgaac cgctcctcgg gcacggcagg caccagcgga cgcggatcct
     4861 ccccgcgcgc cagccgctgc cattccgtcc cgtcatggcc gagacgggat gcgacctcgt
     4921 cggaaggcag cgccgcgaga tcacccagcg tcctcagccc ccagcggcgg agcgtggaca
     4981 gctgctcgat cggatcacga acatttttct ccgtgcctct gtgtctccgt ggtggacgtc
     5041 cgtcttggga gttgggagtt ggccttcgac tgtcgctcag gccaccccga gcatggtcga
     5101 ggggtgggag ttgggagttg acggcgtccg gatggatcgc ggtcagcagg gcgatgggca
     5161 gcgcggccag agtcgcggcc tcggtgcccg gctcgacgac ggtgagcccc gcgcgatgcc
     5221 gcaccagcag ccgtgccgcg gtccgcgtgc cggcaatggc cacgcgcacg cgcaccccgc
     5281 gatcggcggc cgtgcggcgc atgtgttcgg cgattgtccg cgcatcgcca aacaaccgcg
     5341 tcaggccgct cagatcgagg agaacgtccc gcggtgtgtc gatttcgatg cgcggcgaaa
     5401 actcgcgcgc gacgtccgcc aatactccag gcggggttcc ttccggtcca tgcagcgcgc
     5461 caaacatgat gtcaatgcga gcatcgggtc gtgatgacga cgttcccgtc gatccgcttc
     5521 cgcggcgaca cgacacgcac gctgacgtcg gcccccagca ggcggcggct gcgaatggag
     5581 tcgccggccc atgtcgaacg tcccgcaagc gacaacgtca gcccgccggc gctgcgcgcc
     5641 agcggttcct ccgtcaggag gacacatgcc gtgtccctcc cttcgatcgc gcgctgcacg
     5701 cgcggccatg tcgtgaacgg aatctgcctg agctcgcggg gcggcgcgtc cgccagatcg
     5761 atggcgacga tgccgaaccc gcccgcctgc ag
//
LOCUS       DBNGSH010000923         7249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2837140, whole genome shotgun sequence.
ACCESSION   DBNGSH010000923 DBNGSH010000000
VERSION     DBNGSH010000923.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2837140"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2330
                     /locus_tag="AC16CY_21125"
     CDS             <1..2330
                     /locus_tag="AC16CY_21125"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3356369.1"
                     /translation="ELLYKRLPFDDGGRVVRLETRDVAASRVEPRVLHDFAIWRRSLK
                     TVAELGAARLSERNVLTGEGRVESLRVAEITASAFPLTRVQPLLGRALQPADDMQVAE
                     PVVVLGYDVWQRQFLADPAIIGRLVTVGRTARTVVGVMPSRFGFPVNQQLWVPLPVQD
                     AVPREGPAVQVFGRLADGASWQDAATELDVVSARFAADQPATHAQLRTRVRAFAGRTP
                     GDPLRLEDLAVHAIVLLLLGAVSANVMTLVFARTAMRESEIVVRHALGASRARVIAQI
                     VAESLVLTLAAALLGLVVAQTTVRYAWAWAPQTTGDSLPFWVDLTLEPVTVAYALLLA
                     VVAAPMIGLLPALKATRDSVQRGLQGITSTGGTMKFGGIWSFIIGAQVAFTLLFVPAA
                     VGIYTNTVEDQSRWPAFPSERYLTFSLRIDNEALAGEGGVPDDGQIGARRARAYEGLA
                     GLLREEPGVTQVTHGDRLPTMSPEWVAVEMERDGAPPARLQGNFEGGFAMAAVGAGYH
                     DAFGARILAGRGLHTADAGAANGPVVVNEAFMRVLGRNPVGTRVRTVQRGGQRELGPW
                     HEIVGVVTDLEMLFPADWGEAAYIYRAASAAELDPVVVAVRVAGDASPLAPRAAALAP
                     QVDAGLHLRDFVTLDAVVAQERRRRVGGSVIFGSVLLVALVFCAAGLHALMAVAVARR
                     TREIGIRIALGANPRGVLRTVFARAGRQLGGGIVAGNSIVLLIAWRSDSLTANLLVSS
                     VITTVIMAVVGVMACAAPARGALRVQPTETLRQGV"
     gene            complement(2327..2710)
                     /locus_tag="AC16CY_21130"
     CDS             complement(2327..2710)
                     /locus_tag="AC16CY_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356370.1"
                     /translation="MSALKTYRVMLFVSIALNLVIGVFIFVAPDAFANVAGQPEAFPK
                     AWPRHWGLQLWAINLLYLPGLRDPVKHRWPNWLGIAIRLTFATFFFSQGDGFTPMGIY
                     DGASGLLLLVTYLPAVRSAAHQERT"
     gene            2833..3279
                     /locus_tag="AC16CY_21135"
     CDS             2833..3279
                     /locus_tag="AC16CY_21135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356371.1"
                     /translation="MADTIRLVEYFYLTAPNKAGEGARALATLRDAGVNLLAFSGFPQ
                     GRRAQLDLIPADPAALKQAAKKAKWKLVGPKRGFLVQGDDRVGAVADLLDRLGRAKIN
                     VTAIDAVCAGVGRYGAIFWVEPKDVKKAAALLGSASATPSPTSVTL"
     gene            3348..3695
                     /locus_tag="AC16CY_21140"
     CDS             3348..3695
                     /locus_tag="AC16CY_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3356372.1"
                     /translation="MSDKAPSKAGVLQGTLDLMVLQTLDTLGPQHGYAIAARLEQVSG
                     GALQVNMGTLYPALMRLEQRALIRGTWGTTDANRKARFYGLTAAGRRQLAKEKQAWEQ
                     MAGIIHTLLKDES"
     gene            3692..>7249
                     /locus_tag="AC16CY_21145"
     CDS             3692..>7249
                     /locus_tag="AC16CY_21145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3356373.1"
                     /translation="MTRVLREWLQRLRATLVPDRRDEDLEEELRLHLELVAAEARRRG
                     HDPDEAVRMARLEAGGTAQAMEALRDQRGLLWLEDLGRDVRYGLRVLRRSPAFVVVAI
                     LTLALGIGANAAIFHLIDAIRLRSLPIANPDELVDIRADGIDGFGVNSGPNGRVTYPL
                     WEQIRTHQRALSGTFAWGNAGLVIGRGADARRAEGLWVSGDFFRVLGLTPARGRLLTP
                     GDDSPACDAGAAVVSHDYWQASLGGSEAVVGSTLTIVNNVFTVVGVAPAGFSGLEVGR
                     SFDVALPLCVVVRATPDSQRRDSWWLRVMGRVGPGETLAAADAHVRSLSPGILEATIP
                     DGYSAELLERYKSLRFEAVPVGRGVSRLRQTHSMSLSLLLGLSGLVLLITCANLATLM
                     LARAGAREREIAMRVAMGAPRRRLVSQMVVESLLVAAAGAAAAVPIAVASARALVTFL
                     STPDNPISLAVAADWRLVAFVTAAAVLTTLFFGLVPALRVSLVDPVAAMRQTSRGLTV
                     DRHRARLRRGFVAGQIAVSLVLVVSAALFVRSFRNLSAVDIGFDPNGLIVASFFDMGG
                     FAPPERRLAFRQELASAIRSTPGVASVAASMHVPLDGNTWSHFFKLPDVAGDETKVAR
                     FAYISPGYFETMRVKVLAGRGFDEHDRAESRRVLLVNDAFVRRHLGGAQAIGTRVRTA
                     AEAGYPETTYEIVGVVGSTKYGTLRDEDCFCDAGNDAMPPIAYVALAQDPNPLPFVHV
                     IARASGSVSTVAAGIRRRFDAIDPGVGVQVAELAPRIRTLLATERAMAWLAGAFGVLA
                     IVLVVVGLYGVIAYLAVSRRPEVGIRLALGCTRAGIVALVLLDSLWLLAAGAVIGLLL
                     TPTVMRGAGALLFGIGPTDLPTLAIATLVLVIAGAFAGSVPAFRAALLPPMAAIRDEP
                     ESMWRSARAAVQRAVRDLTAGDDTAADTLTSDIAGAIHRAASFQEAVSAALGTLRDRI
                     NARPVLLLQKIGDEYRGSECAIPARGLLINRLTHYPHPLPLTPADIQAWRRWATELRP
                     GHLAEIETLERAGVRIAVPLRTSNELVGVLLLGGPDSREAFTATETKLLSGAASVFAL
                     LIENARLNARELEQEQLRRDLSLAAEVQKRLLPTELPSSEIAEFSAASLPARSIGGDY
                     YDFLDVGSRRLGIALADVSGKGIAAALIMSGVQASLRIMA"
BASE COUNT         1020 a         2430 c         2597 g         1202 t
ORIGIN      
        1 acgaactgct gtacaaacgg ctgccgttcg acgacggcgg ccgggtcgtg cgcctggaga
       61 cgcgtgacgt cgcggcgtca cgcgtggagc cgagggtgct ccacgatttc gcgatctggc
      121 ggcggtccct gaagacggtg gccgagctcg gcgccgcccg cctgagcgag cgcaacgtgc
      181 tcacgggcga aggccgcgtc gaatcgctgc gcgtggcgga gatcaccgcg tctgcattcc
      241 cgctcacccg cgtgcagccg ctgctcggca gggcgttgca gccggcggac gacatgcagg
      301 ttgcggagcc cgtcgtggtc ctcggctacg acgtctggca gagacagttc cttgcggacc
      361 cggcgatcat cggccgcctc gtgaccgtgg gtcgcaccgc tcgcacggtg gtgggcgtga
      421 tgccgtcgcg cttcggcttc ccggtcaatc agcagctctg ggtgccattg cccgtccagg
      481 atgctgtgcc gagggaaggg ccggccgtgc aggtgttcgg ccgcctcgct gacggcgcga
      541 gctggcagga cgccgcgacg gagctcgacg tcgtctcggc gcgctttgcc gccgaccagc
      601 cggcaaccca cgcgcagttg cggacgcggg ttcgcgcatt tgccggacgc acaccgggcg
      661 atccgctgcg gctggaagac ctggcggtcc acgccatcgt cctactgctg ctgggggctg
      721 tgtcggcgaa cgtcatgacg ttggttttcg cgcgtacggc gatgcgcgaa tcggagatcg
      781 tcgtccgcca cgcgctgggg gccagccgtg cccgcgtgat cgcgcagatc gtggccgaat
      841 cgctcgtgct gaccctcgcc gccgccctgc tcggcctcgt tgtggcccag acgacggtgc
      901 ggtatgcctg ggcctgggcg cctcagacga cgggcgacag cctgccgttc tgggtggatc
      961 tgacgctcga gccagtcacc gttgcgtatg cgctgctgct cgcggtcgtc gccgcgccaa
     1021 tgatcggcct gctgcccgcg ctgaaagcca cgagggattc cgtccaacgg ggactgcagg
     1081 ggatcacgag caccggcggg acgatgaagt tcggcggcat ctggtcgttc atcatcggcg
     1141 cgcaggtggc gttcacgctg ctgttcgtgc ccgccgcggt gggaatctac accaacaccg
     1201 tcgaggacca gtcgaggtgg ccagcgtttc cgtcggagcg ttacctcacc ttcagcctca
     1261 ggatcgacaa cgaggcgctg gctggcgaag gaggtgtgcc tgacgatggg cagatcgggg
     1321 cgcgtcgcgc tcgtgcatac gaagggctcg cgggcctgtt acgtgaggag cctggagtga
     1381 cgcaggtgac gcacggcgat cgcctgccga cgatgtctcc ggagtgggtg gcagtggaaa
     1441 tggagcggga cggtgcaccg ccggcgcgcc tgcagggcaa tttcgagggc ggattcgcga
     1501 tggcggctgt cggcgccggt tatcacgacg cgttcggcgc caggatcctg gccggccgcg
     1561 gcttgcatac cgctgacgcc ggtgcggcga acgggccggt cgtggtcaac gaggcgttca
     1621 tgcgcgtgct gggcaggaat cccgtgggca cacgcgttcg cacggtccag cgaggcggcc
     1681 agcgcgagct ggggccctgg cacgagattg tcggcgtggt caccgacctg gagatgttgt
     1741 tccccgcgga ttggggtgag gcagcgtaca tctatcgcgc agcctccgcg gcggagctcg
     1801 atcccgtcgt ggtcgccgtg cgcgtcgcgg gagacgcatc gccgcttgcg ccccgcgccg
     1861 ccgcccttgc gccgcaggtc gatgcgggtc tgcacctccg ggatttcgtc accctcgacg
     1921 ccgtcgttgc acaggagcga agacggagag tgggcggcag cgtcatcttc ggcagcgttc
     1981 tgctcgtcgc gctggtcttc tgcgcggccg gtctgcacgc gctcatggcc gttgccgtgg
     2041 cgcgccgcac ccgcgagatc ggcatccgca tcgcactcgg cgccaacccc cggggcgtgc
     2101 tgcgtaccgt ttttgcccgt gccggtcgtc aactcggcgg cggcattgtc gccggcaaca
     2161 gcatcgtcct gctcatcgcg tggcgctccg acagcctgac cgcgaatctc ctcgtctcgt
     2221 cggtgatcac gaccgtcatc atggcggtcg tcggcgtaat ggcgtgcgcc gcaccggcgc
     2281 gcggggcgct gcgggttcaa cccaccgaga cgctgcggca gggcgtctag gttctctcct
     2341 ggtgtgcggc cgagcgcacg gctggaagat acgtcacgag cagcagcagc cctgacgcgc
     2401 cgtcgtagat ccccatcggc gtgaacccgt caccctggct gaagaagaac gtggcaaacg
     2461 tcagccggat ggcgatcccc agccagttcg gccagcggtg tttcaccggg tcccgcaagc
     2521 ctggcaggta gagcaagttg atggcccaga gctgaaggcc ccagtggcgc ggccacgctt
     2581 tcggaaaggc ttcaggctgt cccgccacgt tcgcgaaggc gtccggggcg acgaagatga
     2641 acaccccgat gacgaggttc agcgcgatgc tgacgaacaa catcactcga tacgtcttca
     2701 gcgcgctcat cggaacagga ggccaattgt ggtcgcctcg caggttgctg tcaacgacgt
     2761 tgactgcgtg cgcccgtgct aggctctcga tctcgggcgc gagtgtgacg cattttcgga
     2821 ggaggctgtt ccatggccga caccatccgt ctcgtcgagt acttctacct cacggcgccg
     2881 aacaaagctg gcgaaggcgc acgggcgttg gcgacgctca gggacgcggg cgtcaacctg
     2941 ctggcgttct ccggatttcc ccagggacgg cgggcgcagc tggacttgat ccctgccgat
     3001 cctgcggcgc tcaagcaggc cgcgaagaaa gcaaagtgga agctggtcgg acccaagcgc
     3061 ggcttcctgg tccagggcga cgatcgcgtg ggggccgtcg cggatctgct cgatcggctg
     3121 ggaagggcga agatcaacgt gacggcgatt gacgccgtct gcgccggtgt cgggcgctat
     3181 ggagcgatct tctgggtcga gccgaaggac gtcaagaaag cggcggcgct ccttggtagt
     3241 gcctcggcga ctccgtcacc tacgagcgtc acgctctagc gggctgagtc cagacgcacg
     3301 gcatcttcca tttgacatcc tgatggagtc ggtgatactc catttgggtg tctgataaag
     3361 ccccgtcgaa agccggcgtc ctccagggca cgctcgacct gatggtgctc cagacgctgg
     3421 acacgctcgg gccgcagcac gggtatgcca tcgccgcgcg cctcgagcag gtgtccggcg
     3481 gcgcgctgca ggtgaacatg ggcacgctct atcccgcctt gatgcggctg gagcagcgcg
     3541 ccctgattcg cggcacctgg ggcaccaccg acgccaatcg caaggcgcgt ttctacggcc
     3601 tcacggcggc aggacggcgg cagctcgcga aagagaagca ggcgtgggag cagatggcgg
     3661 gcatcatcca cacgctgctg aaagacgagt cgtgacgcgc gtcctgcgtg agtggctgca
     3721 gcgactccgc gccacgctgg ttcccgaccg cagagacgag gatctggaag aggagctgcg
     3781 gcttcacctg gagctcgtgg cggcggaggc gcgccgccgc ggccacgatc cggatgaggc
     3841 cgtgcggatg gcgcggctcg aggccggagg caccgcccag gcgatggagg cgctccgcga
     3901 tcagcgcggc ctgctgtggc tcgaggacct gggacgcgac gtgcgatacg ggcttcgcgt
     3961 gctgcggcgg agcccggcgt tcgtcgtggt ggcgatcctg acactggcac tcgggatcgg
     4021 cgccaacgcc gcgatatttc acctgatcga cgcgatcagg ctgcgaagcc tcccgatcgc
     4081 gaatccggac gaactcgtcg acatccgcgc cgacggcatc gacggtttcg gcgtcaactc
     4141 gggccccaac ggccgtgtca cgtatccgtt gtgggagcag atccggacgc accagcgcgc
     4201 cctgtcaggg acgttcgcct ggggaaacgc cgggctcgtc atcgggcggg gcgccgacgc
     4261 gcggcgggcc gaggggctct gggtgagcgg agatttcttc cgcgtgctcg gtctcacgcc
     4321 ggcgcgcggg cggctgctca ccccgggcga cgattcaccg gcctgcgacg ccggcgccgc
     4381 ggtggtgagc cacgactact ggcaggcgtc gctcggcggc agcgaggcgg ttgtcggcag
     4441 cacgctcacg atcgtgaaca acgtgttcac cgtcgtcggc gtcgcgccgg ccggattctc
     4501 ggggctggag gtcggccgct cgttcgacgt cgcgcttccc ctctgtgtcg tcgtgcgggc
     4561 gaccccggat tcgcaacggc gcgacagctg gtggctgagg gtgatgggcc gggtcgggcc
     4621 aggcgaaacg cttgcggccg cggacgcgca cgtgcggtcg ctcagtcccg gcatcctcga
     4681 ggccacgatc ccggacggct acagcgccga actgctcgag cgatacaaat cgctccggtt
     4741 cgaggcagtg cccgtcgggc gcggcgtgag ccgcctgcga cagacgcaca gcatgtcgct
     4801 gtcgctgctc ctgggcctga gcggactcgt gctcctgatc acgtgcgcca acctggccac
     4861 gctgatgctc gcccgcgccg gggcccgcga gcgcgagatc gcgatgcgcg tggccatggg
     4921 cgccccgcgg cggcggctcg tctcgcagat ggtggtggag agcctgctcg tcgccgccgc
     4981 cggcgctgca gccgctgtgc cgatcgcggt cgcgtcggcc agggcgctcg tgaccttcct
     5041 cagcacgccg gacaacccga tcagcctggc cgtcgccgcc gactggcggc tcgtggcatt
     5101 cgtgacggcg gccgcagtgc tgacgacgct gttcttcggc ctcgtgccgg cgctccgcgt
     5161 ctcgctggtc gatccggttg ccgccatgcg gcagacctcg cgcgggctga ccgtcgatcg
     5221 ccatcgcgcc aggctcaggc gagggttcgt ggccggccag atcgcggtct cgctggtcct
     5281 cgtggtctcg gcggcgctct tcgtccgcag cttccgcaac ctgtccgcgg tcgacatcgg
     5341 cttcgacccg aacggcctga tcgtcgcctc cttcttcgac atgggcggct tcgcgccacc
     5401 ggagcggcgc ctggccttcc ggcaggagct ggcgtccgcc atccgttcga cgcccggcgt
     5461 tgcgtccgtg gccgcctcga tgcacgtgcc gttggacggg aacacgtggt cgcacttctt
     5521 caagctgccc gacgtcgcgg gggacgagac caaggtcgcg cggtttgcgt acatcagccc
     5581 cggctatttc gagacgatgc gggtcaaggt gctcgcgggc cgcggtttcg acgagcacga
     5641 tcgcgcggag tcgcgccgcg tgctgctggt gaacgacgcc ttcgtccgcc ggcatctcgg
     5701 aggcgcacag gccatcggca cccgggtgcg gacggctgcg gaggccggat accccgagac
     5761 cacgtacgag atcgtcggcg tggtcgggag cacgaagtac ggcactctgc gggacgagga
     5821 ctgtttctgc gatgccggca acgacgcgat gccgccgatc gcgtacgttg cgctcgccca
     5881 ggacccgaac cctctgccat tcgtccacgt gatcgctcga gcgagcggct ccgtctcgac
     5941 ggtggccgcg ggcatccggc gacgcttcga cgcgatcgat cccggagtgg gcgtgcaggt
     6001 cgccgagctg gcgccgagga tccgtaccct gctcgcgacc gagcgggcga tggcgtggct
     6061 ggctggcgca ttcggcgtcc ttgcgatcgt cctcgtcgtc gtcggcctct acggcgtgat
     6121 tgcgtacctc gccgtgagcc gcaggcccga ggtcggcatc cggctcgcgc tcggatgtac
     6181 gcgagcggga atcgtcgcgc tcgtgctgct cgattcgctc tggttgctgg cggcgggagc
     6241 ggtcatcggc cttctgctca cgcccacggt catgcgtggt gccggagcgc tgctcttcgg
     6301 catcgggccg acagatctgc cgactctcgc gatagcgacg ctcgttctgg taatagcagg
     6361 ggcgttcgcc gggagcgttc ctgcgttccg tgctgcgctg ctgccgccga tggctgcgat
     6421 ccgcgacgag cccgagtcga tgtggcgctc ggcgcgcgcg gcggtccagc gtgccgtgcg
     6481 cgacctgacg gcgggcgacg acacggcggc ggacaccctg accagcgaca tcgcgggcgc
     6541 gattcatcgc gcggcgtcgt tccaagaggc cgtgagcgcg gcgctcggca cgctgcgcga
     6601 tcgcatcaac gcacgccccg tcctgctcct ccagaagatc ggggacgagt accgcggctc
     6661 cgagtgcgcc atcccggcca gggggctgct gatcaaccgg ctcacgcact acccgcatcc
     6721 cctgccgctc acgcccgccg acatccaggc atggaggagg tgggcgacgg agcttcgccc
     6781 cgggcatctc gcggagatcg agacgctcga gcgcgccggc gtccgcatcg ccgtacctct
     6841 gcgtacgtcg aacgagctcg tcggcgtgct tctccttgga ggccccgact cgcgcgaggc
     6901 gttcaccgcc accgagacga agctcctcag cggggcggcg agtgtcttcg cgctgctgat
     6961 cgagaacgcg cgattgaacg cgcgggagct cgagcaggaa cagctgcggc gcgatctgtc
     7021 gctggcggcg gaggtgcaga agcgcctgct gcccaccgag ctcccgtcct cggagatcgc
     7081 cgagttctcg gccgcgagcc tgcccgccag aagcatcggc ggcgactact acgacttcct
     7141 cgacgtcggc agccgccgcc tcggcatcgc gctggcggac gtgtcgggca agggcattgc
     7201 tgcggcgctg atcatgtcgg gggtgcaggc gtcgctgcga atcatggcc
//
LOCUS       DBNGSH010000924         8290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3516362, whole genome shotgun sequence.
ACCESSION   DBNGSH010000924 DBNGSH010000000
VERSION     DBNGSH010000924.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3516362"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC16CY_21150"
     CDS             <1..231
                     /locus_tag="AC16CY_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356374.1"
                     /translation="VQIDYPRYYVPLTLRGKLAVRLRLYRGLLGLLPPSIISLLSRVR
                     SSVLGALTQAPRANGSSDASRLPLSSSSGESR"
     gene            250..321
                     /locus_tag="AC16CY_21155"
     tRNA            250..321
                     /locus_tag="AC16CY_21155"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:282..284,aa:Thr,seq:tgt)
     gene            431..2191
                     /locus_tag="AC16CY_21160"
     CDS             431..2191
                     /locus_tag="AC16CY_21160"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR3356375.1"
                     /translation="MIHHAGFSHAVLSFPHVGACVGTAGFGGTPAPDVALDRHAITAI
                     TARGADVCYRHDAAGDDESPCHARELVKAYEECGGIGLVGFSERCAGFVSDRRRRRCL
                     LFNDAYGSERIFLHVSGSRVFFSSEAKAILTVVPATREFDPLGLAELLACGCTLGTRS
                     LFRNIDVLPGGTVLTFENGAVQRRRYFDPAQLEGLEPVSMPEFVEGFSSSLSTAVNRR
                     LRGSGTVGVSLTGGLDSRMVMASLDAGPGDVPCYTFGSMYRTTGDVSVARQVASACRQ
                     PHHVIELGKEFLDNATEHLENAVYASDGYLGLSGAAELYCNRRARTIAPCRMTGNWGG
                     ELMRGVRAFRYALPKGGFISRDLVDQVHQSAERFAPPSSNSLSAALFQQVPHQGYGRY
                     AIERSQVRVTSPFLDADVVRWLYKAPPAIRESSTTAAAVIARRPELLDIPTDAGLLGV
                     RPSRLRRMVRRSVVKAEYLTSHGAPDWFARISSALPARVLETRFLGVDKFQHFRFWIR
                     HDLAGLVRDTLFGQDRDELSTWFDMRRVRQMVAEHVEGRANHTDAIDKLLTVAVARRT
                     LLGSRPHPSAAEFNQRELAL"
     gene            2188..2952
                     /locus_tag="AC16CY_21165"
     CDS             2188..2952
                     /locus_tag="AC16CY_21165"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3356376.1"
                     /translation="MTSHVIRRLSSKWRRFSVESFGRRDYTLSTDVPYVSFTFDDFPR
                     TALTEGGTILRGHGVTGTYFVSLQLLGKPSPSGPIATPDDVKALLADGHELGCHTYEH
                     LDGCHSSVDEFERSIRQNRAALADVAPGATFPVFAYPLNGPVLSIKRAVGKHFVGCRG
                     GGQTFNSGVTDLNLLKAYFLDWRNRGNADVVREVVERNAAERGWLIFATHDVAPEPSR
                     YGCDPDFFENAVRLSISSGARVVPMMEACAALGIAR"
     gene            2980..4164
                     /locus_tag="AC16CY_21170"
     CDS             2980..4164
                     /locus_tag="AC16CY_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356377.1"
                     /translation="MKPKQILVNVAVILTSLLVGTMLCEVGARLVLNPADYLSVTMLR
                     DPVLGMTIAPGSPGFDKWGFRNPVVPASVDVLAIGDSHTFGNTAKMDEAWPSVVQRQT
                     GLTVYNMGLGGYGPNQYYQVLTTRGLSLHPKRVVCGLYMGDDFENAFTMTYGLDHWAS
                     LRIGQWGKVEADIWGDTEAPGTIKIFRNWLSRNSMIYRLVVHGPALRALKGSIQTRLA
                     NATADPSVTTFASADGTIDEAFRPIRIAAGLDQGRAEVKEGMRLTFHFLQEMNLACRR
                     SGCTFSVVIIPTKETVFAKYLQSDPQLDLKDAVNAVISNEQVATAALEQFLDGAGIPY
                     VVTLPALRERVTERLYYRGPADMHPGPNGYRVIGESVARFLHQPDAAVHGTPDPATAG
                     VP"
     gene            4161..4901
                     /locus_tag="AC16CY_21175"
     CDS             4161..4901
                     /locus_tag="AC16CY_21175"
                     /inference="COORDINATES: protein motif:HMM:NF016668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HIR3356378.1"
                     /translation="MIIVNADDFGRSRAETDAIVACHSARRITSTTAMMFMEDSERAA
                     RTALDLGIDVGLHLNLSQSFTGLRVSEPLRRSHDRIVGFLRATKYALVLYNPFLRRQF
                     RQVVDAQIEEFARLYGKSPAYIDGHHHQHLCANVLFDGIIPRGRRVRRNFTFWPGEKS
                     ALNRWYRRRVDGMLARDYSLVDYFFSLEECLGNGRLSRVFHLAETATVELMTHPVNAS
                     EFEYLMSDAYFDRVRHVGADGRRVRSAS"
     gene            4920..6137
                     /locus_tag="AC16CY_21180"
     CDS             4920..6137
                     /locus_tag="AC16CY_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356379.1"
                     /translation="MSTISGLDVLVGFAEAEAAPEVVWSLVDAGARVTAFARKGVASA
                     LRHSRHVVCRDITPPETDAARAVADLQAVQRDMGQGRTTRLLFPLDDTGVWLCNQVLL
                     DGNWLLAGPRGANVEVALNKGLQVALARECGFNVPESAVVHTRSEAKAFADAQGFPII
                     FKSAHCVPIRDGRRQGSPVWICANEQEFDRTMTQWDARVPLLAQRYIVGTGEGVFGLG
                     AIDGVRGWSAHRRLRMMNPQGSGSSACVSQAVPPDLGASVSALIERTQWRGLFMIELL
                     RDQAGKVWFVELNGRPWGSMALARRQGLEYPAWHVSLAVDERSAAGKTTTSVPGLVCR
                     HLGRELLHPLFVLRGPRSTALGHWPSFWKSMFAVMTIRPRDGIYNWRPSDPSVFLADL
                     CYTLHKNLFKARA"
     gene            6144..6881
                     /locus_tag="AC16CY_21185"
     CDS             6144..6881
                     /locus_tag="AC16CY_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3356380.1"
                     /translation="MDVFAACHLHSEWSYDGSWPIDGLAREFGRRGYRVMMMTEHDNG
                     FTPARYAEFRDACARASSESMYVLPGMEYSDPENRVHVLVWGVPFLGEGLRTEELLDA
                     VAARGGVAVFAHPSRRAAWECFSPKWADSLLGIESWNRKYDGWAPGKSASTLLQASPQ
                     VPFVGLDFHTARQFFPLGMALDVEGPIDEAGVLECLKQRRCAARAFGLPLDERKLHRT
                     LPLLQVAEQGRRGLAALKRYSRSYFPG"
     gene            6895..7794
                     /locus_tag="AC16CY_21190"
     CDS             6895..7794
                     /locus_tag="AC16CY_21190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3356381.1"
                     /translation="MAATPLITVCVCTFKRGEMLEHLLRTLERQRTDGLFTYDVVIAD
                     NDAARSAEPVVRVFAASSSLKVTYCSEPVANIARARNAALARATGDFVAFIDDDEFPT
                     EGWLYSLFRTRETHGADGVLGPVLPHFRLAPPRWLSKGRFFDRPRYPTGRRLAWFEAR
                     SGNVLFARAILMHLDPPFRPQFATAGEDMDFFRRAIEQGRVFIWCDEAEVFEFVPPSR
                     CTRRYLLRRAVLRGSNFPKHPTDRVKNALKSVVALPCYTVALPVLAVVGHHVFMAYLI
                     KVLDHASRLLAFAGLPLMTERET"
     gene            7826..>8290
                     /locus_tag="AC16CY_21195"
     CDS             7826..>8290
                     /locus_tag="AC16CY_21195"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3356382.1"
                     /translation="MALAALESLPRVAVIGMGSWGRNLVRNFQHVGALAAVCDASDAA
                     ETLCRKEYPSVPFVRDYRTVLADPDIAAVAIATPAVTHFDIAKAALEAGKDVFVEKPL
                     STEVSQGEALVRLADDAGRILMVGHILRYHPAVIRLKEMVQNGTLGKINYIYS"
BASE COUNT         1409 a         2570 c         2735 g         1576 t
ORIGIN      
        1 gtgcagatcg actatccgcg ctactacgtg ccgctcacgc tgcgaggaaa gctggcggtc
       61 aggctgcgat tgtaccgagg gttgctcggg cttttgccgc cctcgattat ttcgctcttg
      121 tctcgcgtgc ggtcgtctgt gctcggcgcc ctgactcaag cgccgcgcgc gaacggctca
      181 tcagacgcgt cacgtctccc cctgtcctcg tcgagtggtg agtcgagatg agtcgcgagc
      241 caaacctgtg ccggtgtagc tcaatggtag agcagccgat ttgtaatcgg cggacggagc
      301 gttcgaatcc tcccgccggc tctcattctc aactctagtg gctggcatct tcggcgtagt
      361 cgatacggcg ccggcgggtg ctggaggtca acaggaactg gtcgcaatcg cgagaagaat
      421 gcgagagtcg atgatccacc acgcgggctt ctcgcatgcc gttctgtcct tccctcacgt
      481 cggtgcgtgt gttggtacgg cgggatttgg cggtacgccc gcgcccgacg ttgccctcga
      541 caggcacgcc attacggcca tcaccgcacg tggagccgat gtctgctacc ggcacgacgc
      601 agccggcgac gacgagagtc cctgccacgc acgcgaactc gtgaaggcgt acgaagagtg
      661 cggcggaatt ggtctggttg ggttctcgga gcgctgtgcc ggattcgtgt cggaccgcag
      721 gcgccggaga tgcctgttgt tcaacgacgc atacggaagc gagcgcatct tcctgcacgt
      781 ctcaggcagt cgggtgttct tctcctcgga agccaaggca atcctgacag tggtgcctgc
      841 gacgcgtgag ttcgatccgc tcggcctggc cgaactgctg gcgtgcgggt gcacgctggg
      901 tacacggtcc ttgtttcgga atatcgacgt tctgccgggt ggtacggttc tcacgtttga
      961 aaacggagcg gtccagcgca ggcgctactt cgatccggct caattggagg gactcgagcc
     1021 ggtgtcgatg ccggagttcg tcgaggggtt ctcgagcagt ctgtcgaccg cggtcaaccg
     1081 tcgtctgcgt ggtagcggta cagtcggagt ctctctcacc ggggggctcg actccaggat
     1141 ggtcatggcg tcgctggatg ccggacccgg cgacgtgccg tgttacacgt tcgggagcat
     1201 gtaccgcacc accggagacg tgagcgtggc caggcaggtg gcatcggcct gccgtcagcc
     1261 acaccacgtc atcgagctcg gaaaggagtt tctggacaac gccaccgagc acctcgagaa
     1321 cgcggtgtac gcatccgacg ggtatctcgg gctgtcaggc gcggccgaac tctactgtaa
     1381 tcggcgggcc cgtacgatcg ccccgtgtcg catgactggc aactggggtg gagagctgat
     1441 gcggggtgtt cgcgcgttca ggtatgcgtt gccgaagggc ggattcatca gccgcgacct
     1501 cgtggaccag gtccaccagt ctgcggagag attcgcgccg ccgagctcca attcgttgtc
     1561 cgcggccctt tttcagcagg tcccgcacca gggatacggt cgctacgcga tcgagcggtc
     1621 tcaggtgcgg gtgacgtcgc cgttcctgga cgcagacgtc gttcggtggt tgtacaaggc
     1681 gccgccggcg attcgcgagt cgagtacgac ggccgccgcc gtcatcgcca ggcgtccgga
     1741 actccttgac attcctaccg acgcaggtct gcttggcgtc cggccgtcgc ggttgcggcg
     1801 catggtgcgg cgatctgtcg tcaaggcgga gtatctgacc agtcatggtg ctccggattg
     1861 gttcgcgagg atttcatcgg cgctgccagc ccgggtgctc gagacgcgct tcctgggcgt
     1921 ggacaagttc cagcacttcc ggttctggat tcgccacgac ctcgctggtc tcgttcgcga
     1981 cacgttgttc ggccaggatc gggacgagct ttccacctgg tttgacatgc gtcgggtgcg
     2041 gcagatggtg gccgagcacg ttgaagggcg ggccaatcac acagacgcga ttgacaagct
     2101 gctgacggtt gccgtggcgc gaagaacgct gctggggtct cgcccgcacc catccgctgc
     2161 ggagttcaat cagcgcgagc tcgcgttgtg acatcacacg tgattcgccg gctctcgtcg
     2221 aaatggcgac gcttcagcgt tgaatcgttc ggccgtcgcg actacacgct ctcgacggat
     2281 gtgccgtacg tgtcgtttac cttcgacgac tttccacgga cagctctgac cgaaggcggc
     2341 acgatcctgc gcggacacgg cgtcacaggc acgtacttcg tctcgctcca gctgctcggg
     2401 aagcccagcc cttcgggacc gattgccacg cccgacgacg tgaaggcgct tctggccgat
     2461 ggccacgaac tcggatgcca tacctacgag cacctcgacg ggtgccactc gagcgtcgac
     2521 gagttcgaac gctcgattcg gcagaatcgc gccgcgttgg ccgatgtcgc tccgggcgcc
     2581 acgttcccgg tgtttgccta tccgctgaac ggacccgtgt tgagcatcaa acgagcagtc
     2641 ggcaagcact tcgtgggctg ccgtggtgga ggtcagacct tcaactcggg agtcaccgac
     2701 ctgaacctct tgaaggcgta ctttctcgat tggcggaatc gcgggaacgc ggatgttgtc
     2761 cgcgaggtgg tcgagcgcaa cgccgccgag cgcggatggc tgatctttgc gacgcatgac
     2821 gtggcccccg agccatccag gtacggttgc gacccggact tcttcgaaaa cgccgtccgg
     2881 ctgtccatct cgtcgggggc gcgcgtggtg ccgatgatgg aagcgtgcgc ggcgctcggc
     2941 atcgcgcgat gagaccgctg gcggcacgat ccaggtgaca tgaaacccaa gcagattctc
     3001 gtgaacgtgg cggtgatact gacgtccctt ctggtgggga cgatgctgtg cgaagtgggc
     3061 gcgcggctcg tcctgaatcc ggcagattac ctctcggtga cgatgcttcg agatcccgtt
     3121 cttggcatga ccatcgcgcc gggctcgccc ggcttcgaca aatggggctt cagaaaccct
     3181 gtcgtgcccg cgagcgtcga tgtcctcgcc atcggcgact cgcacacgtt tggcaatacc
     3241 gccaagatgg acgaagcatg gcccagcgtc gtccagcgac agaccggtct tacggtctac
     3301 aacatgggtc tgggtggata cggaccgaac cagtactacc aagtactcac aacacgtggg
     3361 ctgtcgcttc atcccaagcg cgtcgtctgt ggactgtaca tgggcgacga cttcgaaaac
     3421 gcgttcacca tgacctacgg gctcgatcac tgggcgtctc tccgtatcgg ccaatggggg
     3481 aaagtggagg ccgacatctg gggcgacacg gaggccccgg gcaccatcaa gatcttcagg
     3541 aactggctct cacgaaacag catgatctat cggctcgtcg tccacggccc cgcgttgcgc
     3601 gcgctcaagg gcagcataca gaccaggctc gccaacgcca ctgccgatcc gtcggtgacg
     3661 acgttcgcga gcgccgatgg gaccatcgac gaggcgttcc ggcccattcg aattgccgcc
     3721 ggtctcgacc aggggcgtgc cgaagtcaag gagggcatgc ggctcacgtt ccatttcctg
     3781 caggaaatga atctggcgtg ccgaaggagc gggtgcacgt tttcagtggt gatcatcccg
     3841 acgaaggaaa ccgtcttcgc caagtatctg caatccgacc cacagctcga cctcaaggac
     3901 gccgtcaacg ccgtcatctc caacgagcag gtcgcgactg cggcgctcga gcagttcctc
     3961 gatggcgcgg ggattccgta tgtggtcacg ctgccggcgc tgagagagcg ggtgacggaa
     4021 cgactgtatt accgcggacc cgcggacatg catcccggtc cgaacggcta ccgggtcatc
     4081 ggtgaaagcg tggccaggtt tcttcatcag ccggacgccg ccgtgcacgg aacccccgat
     4141 cccgcgacgg caggtgtgcc gtgatcatcg tcaacgctga cgacttcggg cgctcgcgcg
     4201 cggagaccga cgcgattgtg gcttgccaca gcgctcgtcg tattacatcg acgacggcca
     4261 tgatgttcat ggaagactcc gagcgggccg ctcgaacggc gctggacctc ggcatcgatg
     4321 tagggctcca cctcaacctc tcgcagagct tcacgggcct ccgcgtgtcg gagcctctac
     4381 gccggtcgca cgatcgcatc gtcggtttcc tgagagcgac caagtacgcg ctcgtgctgt
     4441 acaacccgtt tctccgccgc cagtttcgcc aggtcgtcga tgctcaaatc gaggagttcg
     4501 cgcggctcta tggaaagtcg cccgcgtaca tcgacggaca tcaccatcaa cacctgtgcg
     4561 cgaacgtcct gttcgacgga atcattccgc gcgggcgtcg cgtgcgccgc aatttcacct
     4621 tctggcccgg ggagaagagc gccctgaaca ggtggtaccg ccgaagggtt gacgggatgc
     4681 tggcgcgcga ctactcgctg gtcgactatt tcttctcgct ggaggagtgc ctcgggaacg
     4741 ggcgtctctc ccgggtattt catctggcgg aaacggccac cgtcgagctc atgacgcacc
     4801 cggtgaacgc gagcgagttc gagtatctga tgagcgacgc ctacttcgac cgcgtgcgcc
     4861 acgtcggagc ggatggacgc cgtgtacgca gcgcaagtta gcggccgcgc ctgcagttgg
     4921 tgagtacgat ctcgggtctg gacgtgctgg tcggctttgc cgaggcggaa gcggcgccgg
     4981 aagtcgtgtg gagcctcgtg gacgccgggg cccgcgtgac cgcgttcgcg cgcaagggcg
     5041 tggcgtcggc cctgcgccac agccggcatg tcgtctgcag agacatcacg ccaccggaaa
     5101 ccgacgccgc acgcgccgtc gcggacttgc aggccgtcca acgagatatg gggcagggta
     5161 gaaccacgcg cttattgttt ccgctcgacg acaccggcgt ctggctgtgc aatcaggtcc
     5221 tgctggacgg gaactggctc cttgccggtc ctcgcggcgc gaacgtcgaa gtggcgttga
     5281 acaaagggct gcaggtggca ctcgcgcggg agtgtggctt caacgtaccc gagtccgccg
     5341 tcgtccacac ccggtcggag gccaaggcgt tcgcagacgc ccagggattt ccaatcatct
     5401 tcaagtccgc acactgcgtg cccattcgtg acggccgccg gcagggaagc ccggtgtgga
     5461 tctgcgccaa tgagcaggag ttcgaccgca ccatgacgca gtgggacgct cgagttccgc
     5521 tgctcgccca acgctacatc gtgggcacgg gagagggtgt gttcggactg ggtgcgatcg
     5581 acggcgtgcg ggggtggagc gcgcatcgca ggctgaggat gatgaaccct caaggctcgg
     5641 gttcgagcgc ctgcgtatca caagccgtcc cgcccgacct cggcgccagt gtatcggcgt
     5701 tgatcgagag gactcagtgg cgtgggttgt tcatgatcga gctcttgaga gaccaggcgg
     5761 gcaaggtctg gttcgtcgag ctgaatggaa ggccctgggg aagcatggcg ttggcgcgca
     5821 gacagggcct ggaatatccg gcatggcatg tgagccttgc cgtggatgaa aggtccgccg
     5881 cgggcaaaac gacgacatcc gtgccaggcc tggtctgcag gcatctcggc cgcgagctgc
     5941 tgcacccgct gttcgtgttg cgtgggccca ggtcgacggc gcttgggcac tggccctcgt
     6001 tctggaagtc gatgttcgcg gtcatgacga ttcgccctcg agacggcatc tacaactggc
     6061 ggccgtccga tccgagcgtg ttcctcgcgg acttgtgcta cacgctgcac aagaacctgt
     6121 tcaaggcgag agcctgattg gccgtggacg tattcgccgc ctgtcacctg cattcagagt
     6181 ggtcttacga cggaagctgg ccgatcgacg gcctggcgcg cgagttcggc cggcgcggct
     6241 atcgagtgat gatgatgacc gagcacgaca atggcttcac tccggcccga tacgccgagt
     6301 tcagagacgc gtgtgcgcgc gcgtcatccg agagtatgta cgtgctcccc gggatggagt
     6361 acagcgatcc ggagaaccgc gtacacgtgc tcgtgtgggg agtgccgttt ctgggcgaag
     6421 gactgcgcac ggaagagttg ctcgatgccg tggcggctcg cggaggcgtg gctgtgttcg
     6481 ctcacccatc tcgacgagct gcatgggaat gcttctcgcc gaagtgggcc gacagccttc
     6541 tcggcatcga gtcgtggaac cggaagtacg atggctgggc ccccggcaag agtgcgtcca
     6601 cgctcttgca ggcgagtccg caggttccgt tcgtcgggct ggatttccac accgcgcgcc
     6661 agttctttcc tctcggaatg gcgctcgacg tcgagggccc gatcgacgaa gccggcgtgc
     6721 tcgagtgcct caaacagcgc cgctgcgcgg ctcgcgcgtt cgggctgccg ctcgacgagc
     6781 gaaagctgca caggacgctt cccttgctgc aggttgccga gcagggacgg cgggggcttg
     6841 cggccctgaa gcgatactcc aggagctatt tccctggtta aggaagccgg tcaattggcg
     6901 gccactcctc tcatcacggt gtgcgtctgc acgttcaagc gcggcgagat gctggaacat
     6961 ctcctgcgca cgcttgaacg tcaacggacc gacggcctgt tcacttacga cgtcgtgatc
     7021 gccgacaacg acgcggcccg gtcagccgag cctgtcgtgc gcgtgttcgc ggcgtcatcc
     7081 agtctgaagg tgacgtactg ttcggaaccg gtcgccaaca tcgcacgcgc acggaacgcc
     7141 gcattggcgc gtgccaccgg cgatttcgtc gcgttcatcg atgacgacga gttcccgacg
     7201 gagggatggc tgtactcgct gttccgcacg cgcgagaccc acggtgcgga cggcgtgctg
     7261 ggcccggtcc tgccgcactt ccggctcgcg ccaccgcgtt ggttgagcaa gggcaggttc
     7321 ttcgatcgcc cgcggtatcc aacgggacgg cgcctggcgt ggttcgaggc tcgcagcggc
     7381 aacgtgctgt tcgcgcgcgc gatcctgatg cacctcgacc cgccctttcg cccgcagttt
     7441 gcgaccgccg gcgaggacat ggacttcttc cgccgcgcga tcgagcaggg gcgcgtgttc
     7501 atctggtgcg acgaggcaga ggtgttcgaa ttcgtcccgc cctccaggtg cacgcggcgc
     7561 tacctgctga gaagggccgt ccttcgcggc agcaacttcc cgaagcaccc gaccgatcgc
     7621 gtgaagaacg cgctgaaatc ggtggtcgcg ctgccctgct acaccgtcgc gctgcccgtg
     7681 cttgcagtcg tgggacatca cgtgttcatg gcgtatctga tcaaggtgct ggaccacgcg
     7741 tcacgccttc tcgccttcgc gggattgccg ctcatgaccg agcgcgagac gtagcttcac
     7801 aactgggaca caacaaggag aaggaatggc gttagccgca ctggaatcgt taccgagagt
     7861 cgccgtcatc ggaatggggt cgtggggacg caacctggtc cgcaactttc agcacgtcgg
     7921 tgcgcttgcg gccgtctgcg acgccagcga cgcggctgag acgctgtgcc ggaaagagta
     7981 cccgtcggta ccgtttgtcc gcgactaccg cactgtcctg gccgatcccg acatcgcggc
     8041 ggtggcgatc gcgacgccgg cggtgacgca cttcgacatc gccaaggctg ctctcgaggc
     8101 gggcaaggac gtgttcgtcg agaagccgct gtcgaccgaa gtctcacaag gcgaggcgct
     8161 cgtacggctt gccgatgacg ccggtcggat tctgatggtc ggccacatcc ttcgctacca
     8221 tccggcggtg attcgcctga aggagatggt ccagaacgga acgctcggga agatcaacta
     8281 catctactcg
//
LOCUS       DBNGSH010000925         1516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3855492, whole genome shotgun sequence.
ACCESSION   DBNGSH010000925 DBNGSH010000000
VERSION     DBNGSH010000925.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3855492"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1516
                     /locus_tag="AC16CY_21200"
     CDS             <1..>1516
                     /locus_tag="AC16CY_21200"
                     /inference="COORDINATES: protein motif:HMM:NF014731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIR3356383.1"
                     /translation="LVISVKYIVVVMRADNQGEGGILSMTALVPTGNGVRSGRYSLLV
                     GLGIVGASLLLGEGMITPPISILGAIEGLQVATPVFTPWVVPLSVGILVGLFLIQRHG
                     TQRVGGFFGPVMVVWFLTIAGLGVTWIARSPEVLWAFDPRHAVAFFVEHGWHGFAVLG
                     AVVLVVTGAEALYADMGHFGRRPIRLAWFLLVLPALLINYFGQGGLLLERGEAASRPF
                     FQMVPAWGLLPLVALSTTAAIIASQALISGAFSLARQAIQLGYAPRLDIQHTSSSTIG
                     QIYVPQVNWFLAISTVLIVIGFRSSSALAGAYGIAVTGTMVITAVLLSVVAIERWKWP
                     KAAVYSVAAVFLLVDLAFFGANMLKVLHGGWLPLVIGALIFTMMTTWKTGRRLVAERL
                     TARALPLEDFMAAVTTTPPTRVPGTAVFMTAQPRGTPPALAHNVRYNKILHEHVVVLT
                     VTTAPIPYVEADRQVQAQVLGHGIYNVRAQYGFMQDPDVPDALLKAREQGVELDL"
BASE COUNT          216 a          501 c          499 g          300 t
ORIGIN      
        1 ctcgtcatct cggtcaagta catcgtcgtc gtgatgcgcg ccgacaacca gggggagggc
       61 ggcattctct ccatgactgc cctcgtgccc accgggaacg gcgtccgttc ggggcgttac
      121 tcgcttctcg tcggactggg gatcgtcgga gcctctctgc tcctgggcga aggcatgatc
      181 acgcctccca tcagcatcct gggcgcgatc gagggcctgc aggtcgcgac accggtcttc
      241 acgccgtggg ttgtccctct ctcggtcggg attctcgttg gtctcttcct gatccagcgg
      301 catggcacgc agagagtcgg agggtttttt ggaccggtga tggtggtgtg gtttctcacc
      361 atcgccggac tgggcgttac ctggatcgcc cgctcacccg aggtgttgtg ggcgttcgat
      421 ccgcgccacg cggtcgcctt cttcgtggag cacggctggc acggattcgc ggtgctcggc
      481 gccgtcgtcc tggtggtgac cggagccgaa gcgctgtacg cggacatggg ccacttcgga
      541 cggcgaccga ttcggctcgc atggtttctc ctcgtgctgc cggccctgct catcaactac
      601 ttcggtcagg gcggactgct gctcgagcgc ggtgaagccg cctcgcggcc gtttttccag
      661 atggtgccgg cgtggggact tcttccgctg gtcgccttgt cgaccacggc cgccatcatc
      721 gcgtcccagg cgctcatctc gggcgcgttc tctctggcgc gccaggcgat tcagctgggc
      781 tacgcacccc ggctggacat tcaacacacg tcctcgagca cgataggcca gatctacgtg
      841 ccgcaggtga actggttcct cgcaatcagc accgtgctca tcgtgatcgg cttccggtcg
      901 tcgagcgccc tggcgggagc gtacgggatt gccgtcacgg ggacgatggt gatcacggcg
      961 gtcctgctgt cggtcgtggc cattgaacgc tggaagtggc ccaaggccgc cgtctactcc
     1021 gtcgcggccg tgtttctact tgtcgatctg gcattcttcg gagccaacat gctgaaggtg
     1081 ctgcacggcg gctggcttcc gctcgtcatc ggcgcgctga tcttcacgat gatgacgacg
     1141 tggaagaccg gacggcgcct cgtcgcggaa cgattgacgg cgcgcgcgct gccgctcgag
     1201 gacttcatgg cagcggtgac gacaacgccg cccacccgag tgcccggcac ggcggtcttc
     1261 atgaccgcac agccgcgcgg cacgccgccg gctctggcgc acaacgtccg ctacaacaag
     1321 attctccacg agcacgtcgt cgtgctcacg gtgaccacgg cgcccattcc gtacgtcgag
     1381 gcggaccggc aggtccaggc acaggtgctg ggccacggga tctacaacgt gcgcgcgcag
     1441 tacggcttca tgcaggatcc ggacgtcccg gacgcgctgc tgaaggcccg cgaacagggc
     1501 gtcgagctcg acctcg
//
LOCUS       DBNGSH010000926         3701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1648905, whole genome shotgun sequence.
ACCESSION   DBNGSH010000926 DBNGSH010000000
VERSION     DBNGSH010000926.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1648905"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="AC16CY_21205"
     CDS             <1..340
                     /locus_tag="AC16CY_21205"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3356384.1"
                     /translation="AHHSFSAEYDSAKPLKMSGKVTEMRWANPHAWIYIDVVGKDGKA
                     VNWAWETGGANALYRRGWRKEDLVAGTMIVIDGFQARNGSRTANATSITFPDGRKLFA
                     GSSSQNPATK"
     gene            complement(371..1093)
                     /locus_tag="AC16CY_21210"
     CDS             complement(371..1093)
                     /locus_tag="AC16CY_21210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3356385.1"
                     /translation="MTSSAGHFLFKVRDALFPVLLLLAGFGTRPQLAGESVQLDHWMD
                     ALGVAVALAGQAWRFLVVGLDYIARGGKNRQVWANTLVDGGMFAHSRNPLYVSNVIII
                     LGLAIVHNGWAMYALVPVFMLVYVAIVSAEEEYLSARFGDAYAQYCKRVPRWLPSSGG
                     MLTTIRSSQFDWLKVLRKEYGTVFAWTSGVLVFMVWEHYSPSAPPIGSNELYVIAATW
                     IGLAVAYVVTRTLKLRGRLGPP"
     gene            complement(1119..1883)
                     /gene="cmoA"
                     /locus_tag="AC16CY_21215"
     CDS             complement(1119..1883)
                     /gene="cmoA"
                     /locus_tag="AC16CY_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007802201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HIR3356386.1"
                     /translation="MDLPKKASPETSAADRLFAERRELAKDFDFGAETAAVFDDMLDR
                     SVPFYGELQRMTGELAADFATQGSSIYDLGCSTANTFLSIGAHLTPDRDLTFIGLDSS
                     EEMLSRAGAKLAGARFPWPYSLRRFDLNESLRIENASVVLMVLTLQFVRPLNRESLLK
                     SVYDGMNHNGCLVLIEKVLSPHTTFNRLFIDHYYQMKKRKGYSDLEIAQKREALENVL
                     VPYHLEENQQLLRTCGFQHLDVFFKWYNFCGILAIK"
     gene            1992..2960
                     /locus_tag="AC16CY_21220"
     CDS             1992..2960
                     /locus_tag="AC16CY_21220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HIR3356387.1"
                     /translation="MAVDVSPSQLAARGAWDILLAAASLAESLDRRGHDAHFGLRGSA
                     ELQPLASNDTGVVLAWSPDTGWQSRLPSQDARSELLNLYLPICSATSARPVTVGHLGQ
                     SLDGFIAMHSGESQFVTGDDNIIHLHRMRALCDAVVVGAGTVAADDPQLTVRHVPGPN
                     PLRVIIDPGRRLAPTFRVFTDQSGPTLYFCSRTLIRPGESHLGSAAIVGVDSDEPGGG
                     VGEVMRRLHARGCARVFVEGGGVTVSAFLEADLLDRLQIAIAPVLIGDGRPAIRLAPH
                     ARLRDCRRPRYRVFRMGGDVMFDCELKGSQDDNGASPETPVVSRVI"
     gene            complement(2944..>3701)
                     /locus_tag="AC16CY_21225"
     CDS             complement(2944..>3701)
                     /locus_tag="AC16CY_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase type 12"
                     /protein_id="HIR3356388.1"
                     /translation="HVLDLCTGTGSNLRYLIGRLPDRQRWLVVDRDGSLLEEVPGRLG
                     LWAGERGLTTGTDSTGVHIRGSRLECDVEIRQMNVASLDAGIFEGRHLVTASALLDLA
                     SESWLTLLAERCRAVRASALLAITYDGSSSCDPPEPEDEIVLGLFNQHQRTDKGLGGP
                     AGGPGAWAAAVRSFTDAGYLVESASSDWVLKPADRAFQRQLIEGWARAAAEIAPERTA
                     LVDDWLRRRQIHVDAGRSRIIVGHRDMAARLLG"
BASE COUNT          634 a         1228 c         1231 g          608 t
ORIGIN      
        1 cgcgcatcat tcgttctcgg cggagtacga ctcggcgaag ccgctgaaga tgagcggaaa
       61 agtcacggag atgcggtggg cgaaccctca cgcgtggatc tacatcgacg tcgtgggcaa
      121 ggacggcaag gccgtcaact gggcctggga gaccggcggc gccaacgctc tctaccggcg
      181 gggctggcgg aaggaggatc tggtcgcggg cacgatgatc gtcatcgacg gcttccaggc
      241 tcgtaacgga tcgagaaccg ccaacgcgac gagcatcacc tttcccgacg ggcggaagct
      301 gttcgcgggt tcgtcgtcgc agaacccggc caccaagtag aacagggggc agggggcagg
      361 gggcaggggt tcacggcggc ccgaggcggc cgcgcagctt gagagttcgc gtcaccacgt
      421 acgccacggc gagcccgatc cacgtcgccg cgatcacgta gagttcattg gagccgatgg
      481 ggggcgccga cgggctgtag tgctcccata ccatgaacac gagaacaccg ctcgtccacg
      541 cgaagaccgt tccgtactcc ttccgcagca ctttcagcca gtcgaactgg ctgctgcgta
      601 tcgtcgtcaa cataccgccg gacgacggca gccatcgcgg cacccgcttg cagtactggg
      661 cgtaggcgtc gccgaaccgc gcgctcaggt actcctcttc ggcgctcacg atggccacgt
      721 agaccagcat gaacacgggc acgagtgcgt acatcgccca cccgttgtgg acgatcgcga
      781 gtcccaggat gatgatcacg ttggagacgt acagcgggtt gcggctgtgc gcgaacatcc
      841 cgccgtcaac gagcgtattg gcccagacct gacggttctt tcccccgcga gcgatgtagt
      901 ccaacccgac gacgaggaaa cgccacgcct ggccggcgag cgcgacggcg acgcccaggg
      961 cgtccatcca atgatcgagt tgaacgcttt cccctgcgag ctgcggcctg gtgccaaagc
     1021 cggcgagcag cagcagcacg ggaaagagcg catcccgtac cttgaacagg aagtggcctg
     1081 ccgaggatgt caagccgcga ggtgggacgg ccgcctcgct acttgatcgc gaggatcccg
     1141 cagaagttgt accacttgaa gaagacatcg aggtgctgaa aaccgcaggt gcggagcaat
     1201 tgctggttct cttcgagatg gtagggcacg aggacgttct cgagcgcctc gcgcttctgc
     1261 gcgatctcga ggtcgctgta gcccttgcgc ttcttcatct ggtagtagtg gtcgatgaac
     1321 agccggttga acgtcgtgtg cgggctcagc accttctcga tgaggaccag gcagccgttg
     1381 tggttcatgc cgtcgtacac gctcttcagc agcgactcgc ggttgagcgg ccgcacgaat
     1441 tgcagcgtca acaccatcag cacgaccgat gcgttttcaa tccgcaggct ctcgttcagg
     1501 tcgaaccgcc tgagcgagta cggccagggg aagcgcgcgc cggcaagctt ggccccggcg
     1561 cggctcagca tctcctcgga cgagtcgagc ccgatgaagg tgaggtcgcg gtccggcgtg
     1621 agatgggcgc cgatggagag gaacgtgttc gccgtggagc agccgaggtc gtagatcgac
     1681 gagccctggg tggcgaagtc ggcggcgagc tcgccggtca tgcgctggag ctccccgtag
     1741 aaggggaccg accggtccag catgtcgtcg aagaccgcgg ccgtttcggc gccaaagtcg
     1801 aagtctttcg ccagctcgcg ccgttcggcg aagagtcggt ccgcggccga cgtttccggg
     1861 ctggccttct tcggcaggtc caccgcgaaa cagaatacca aaccttcttg gcattcgcct
     1921 gagctcgtgt ggcatagtga cgccatgcac gccggtcgac ctcccgccag ccggcaccgg
     1981 agcgtcgctc agtggcagta gacgtatcgc ccagccagct cgccgcgcgc ggcgcgtggg
     2041 acatcctgct ggccgcggca tcgctggccg agtcgctcga ccgtcggggg cacgacgctc
     2101 acttcggtct tcgcggatcg gctgaacttc agccgctcgc gtcgaacgac accggtgtcg
     2161 tcctcgcctg gtcgcctgac acgggatggc agtcgcgcct gccctcgcag gacgcccggt
     2221 ccgagctgtt gaatctgtat ctccccatct gcagcgcaac ctcggcgcgc ccggtcaccg
     2281 tgggccacct tgggcagagc ctcgacgggt tcatcgcgat gcactccggc gagtcgcagt
     2341 tcgtcaccgg agacgacaac atcatccatc tccaccgcat gcgcgcgctg tgcgacgcgg
     2401 tcgtcgtcgg ggccggcacc gtggcggccg acgacccgca actgaccgta cgacacgtcc
     2461 cgggtccgaa tcctctcagg gtcatcatcg atcccggacg ccggctggcg ccgacgttcc
     2521 gggttttcac cgatcagagc gggcccacgc tgtatttctg ttcacggacg ctcatccgcc
     2581 ccggcgagag ccacctgggg agcgcggcca ttgtcggcgt ggacagcgac gagcccggcg
     2641 gcggtgttgg cgaggtgatg cggcggctgc acgcgcgcgg gtgcgcacgc gtcttcgtcg
     2701 aagggggcgg cgtgacggtc tcggcgtttc tcgaggccga tctgctcgac cgcctgcaga
     2761 tcgcgatcgc tccggtgctc atcggagacg gccggccggc aatccgtctt gcgccgcacg
     2821 cgcgcctgcg cgattgccgc cgccctcggt accgtgtgtt ccgtatgggc ggcgacgtca
     2881 tgttcgactg cgagctcaaa ggaagccagg acgacaacgg cgcgtccccg gaaacgcccg
     2941 tggtcagccg agtaatctag cggccatgtc gcggtggccc acgatgatcc gggagcggcc
     3001 ggcatcgacg tgaatctgcc ggcggcgcag ccagtcatcg acgagcgccg ttcgttcggg
     3061 cgcgatctcg gcagcggcgc gtgcccatcc ttcgatcaac tgccgctgga atgcgcgatc
     3121 cgccggcttc aacacccagt cgctcgaagc gctctccacc aggtacccag catccgtgaa
     3181 actgcgcacg gccgcggccc acgccccggg tccccccgct ggtccgccga gccccttgtc
     3241 cgttcgttga tgctgattga acagacccag aacgatctcg tcctccggct cgggtgggtc
     3301 acaagacgaa ctgccgtcgt acgtgatggc caggagcgcc gacgcgcgaa cggccctgca
     3361 gcgctcggcc agcagggtga gccagctttc ggacgcgaga tcgagcaacg ctgatgcggt
     3421 gaccaggtgg cgtccctcga agatccccgc gtcgagcgag gcgacgttca tctgccggat
     3481 ctcgacgtca cactccaggc ggctgcctcg tatgtggacg cccgtcgagt cggtcccggt
     3541 cgtgagacct cgctcgccgg cccagagccc cagccgtccg ggcacttcct cgagtaacga
     3601 cccgtcgcgg tcaacgacca gccagcgctg ccggtccgga agacgaccga tgaggtagcg
     3661 caggttcgag ccggtgcccg tgcacaggtc gagcacgtga a
//
LOCUS       DBNGSH010000927         3061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4196914, whole genome shotgun sequence.
ACCESSION   DBNGSH010000927 DBNGSH010000000
VERSION     DBNGSH010000927.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4196914"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="AC16CY_21230"
     CDS             <1..534
                     /locus_tag="AC16CY_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR3356389.1"
                     /translation="AKEYGYRTDRIAISGASSGAHLAALVGVTNGNKELEGTLGNYPK
                     ESSDVQAIIDYFGASNLTTILPQSTPFGLNVRKPALDLLLGGAPEDASIKALAELASP
                     VKHVDKGDPPLLLFHGDQDPQMPINQAHELHAAYKKAGLDVAFTVVHGAAHGGNQFFA
                     PDNLQQALAFLNRTIGK"
     gene            544..1731
                     /locus_tag="AC16CY_21235"
     CDS             544..1731
                     /locus_tag="AC16CY_21235"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3356390.1"
                     /translation="MPQSSTLTHLECSRPTCRKVADCSVRQNLCPVCGAPLLARYDLA
                     AARRTLTRAALDTRVHSMWRYEEVLPRARPVTLGEGLTPLVHARRLGAHLGLDRLYVK
                     DEGQNPTASFKARGLSAAVSMARALGVEAIAIPSAGNAGAAAAAYAAQADLSCVVAMP
                     ADTPAAIVLETRAFGADVHLIDGLISDCGTFIAERATANGWFEVSTLKEPYRIEGKKT
                     MGYELWEQFEGTLPDVIVYPTGGGVGLIGMWKAFDELETMGLVDSRRPRMIAAQAEGC
                     APIERAFARRADVSERWEQATTVAAGLRVPKPLGDFLILADIYASGGEAVAASDEALM
                     EACHDLARLEGILAAPESGAGLVAIRKLVEQAKIRRDESVVLFNTGSGYKYLEAWQTG
                     ETR"
     gene            1728..2099
                     /locus_tag="AC16CY_21240"
     CDS             1728..2099
                     /locus_tag="AC16CY_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3356391.1"
                     /translation="MSVRFQSLRPMLRTKDLHATVTFWTDCLGFSCKGLSEEDGWASL
                     CRDHVSVMIATPNAHVPFETATFTGSFYVDVDNVTELWADLKDRVRIAYPMEDFEYGM
                     REFAIYDNNGYMLQFGSPVRS"
     gene            complement(2121..3056)
                     /locus_tag="AC16CY_21245"
     CDS             complement(2121..3056)
                     /locus_tag="AC16CY_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3356392.1"
                     /translation="MARLGRTDLDVFPLCLGGNVFGWSSSEQESFAVLDAYAAAGGNF
                     IDTADSYSFWVPGHRGGESETILGRWMAARGARNRVIVATKVGRAPGLNGLSPKTIRT
                     AAEASLRRLGSDRIDLYYAHADDPNTPIEETLRAFDGLVREGKVRYIAASNFTAPRLS
                     EALAISTREGLSRYVALQPHYNLVHRGDYEGPLAELCAREGLACLPYFALAQGFLSGK
                     YRPGTTVESVRAEGARAYLDARGRRVLGVLDDVAAAHKVSVAAVSLAWLLSQPTVGAP
                     IASARTVAQLAELVQVATLTLNGEEIQRLTDASRP"
BASE COUNT          513 a         1010 c         1043 g          495 t
ORIGIN      
        1 gccaaggagt acgggtaccg gacggatcgc atcgcgatct ccggcgcctc gtcgggcgcg
       61 catcttgccg cgctcgtcgg cgtgaccaac ggcaacaagg aactcgaggg gacccttggc
      121 aattacccga aggagtcgtc cgacgtccag gcgatcatcg actacttcgg cgcatcgaac
      181 ctgacgacga tcctgcctca gtccactccg ttcggcctga acgtgcggaa gccggcgctg
      241 gatctgcttc ttggcggggc acccgaagac gccagcatca aggcgctcgc cgaactggcc
      301 agccccgtca agcacgtgga caagggcgat ccgccgctgc tcctgttcca cggcgatcag
      361 gatccccaga tgccgatcaa ccaggcacac gagctgcacg ccgcctacaa gaaggccgga
      421 ctcgacgttg ccttcaccgt cgtccacggg gcggcgcacg gcggcaacca gttcttcgcg
      481 ccggataacc tccagcaggc gctcgcgttc ctgaatcgca cgatcggaaa gtaagaggct
      541 cgaatcccgc agtcctcgac tctcacgcac ctggagtgct ccaggccgac gtgccgcaag
      601 gtcgccgact gttccgtcag gcagaacctg tgcccggtct gcggggcccc gcttcttgcg
      661 cgttacgacc tggccgccgc gcgccggacg ctgacgcgcg ccgcgctcga cacgcgcgtg
      721 cactcgatgt ggcgctacga ggaggttctg ccgcgcgcgc gccccgtcac gctcggtgaa
      781 gggctgacgc cgctcgtcca cgcgcgtcga ctcggcgcgc acctcggcct cgacaggctc
      841 tacgtcaaag acgaagggca gaaccccacg gcgtccttca aggcccgcgg cctgtcggcg
      901 gcggtcagca tggccagggc gctcggcgtc gaggccatcg cgattccaag cgcgggcaac
      961 gccggcgccg cggccgcggc gtacgccgcc caggcggatc tctcgtgcgt cgtcgccatg
     1021 cccgcggaca cgcccgccgc catcgtcctc gagacgcgcg cgttcggcgc ggacgtgcat
     1081 ctcatcgacg ggctgatttc cgattgcggg acgttcatcg ccgagcgggc gacggcgaac
     1141 ggctggttcg aagtgtcgac gctgaaggag ccgtatcgca tcgaaggcaa gaagacgatg
     1201 gggtacgagt tgtgggagca gttcgagggc acgctccccg acgtgatcgt gtatcccacc
     1261 ggcggcggag tcggcctcat cgggatgtgg aaggcgttcg acgagttgga gacgatgggc
     1321 ctcgtggaca gccggcggcc gcgcatgatc gcggcgcagg ccgagggctg cgcgccgatc
     1381 gagcgggcat tcgcgcggcg cgcggacgtt tcggagcgat gggagcaggc gacgaccgtt
     1441 gcggcgggac tgcgtgtgcc caagcctctc ggcgatttcc tgattcttgc cgacatctat
     1501 gccagcggcg gcgaagccgt ggcggcgagc gacgaggcgt tgatggaggc gtgccacgat
     1561 ctggctcgcc tcgaaggcat tctggcggcg ccggagtcgg gcgcggggct ggtcgcaata
     1621 cggaagctcg tcgagcaggc caagatccga cgggacgaat ccgtcgtcct cttcaacacg
     1681 ggaagcggat acaagtatct cgaggcgtgg cagacggggg agacacgatg agcgtacgct
     1741 ttcagtcgct gcggccgatg ctgcgaacca aggatctgca cgcgacggtg acattctgga
     1801 cggattgtct ggggttcagc tgcaagggcc tgagcgagga agacggctgg gcctccctct
     1861 gccgcgacca cgtgagcgtg atgattgcca ccccgaacgc ccacgtccct ttcgagaccg
     1921 cgaccttcac cgggtccttt tacgtcgacg tcgacaatgt gaccgagctg tgggccgacc
     1981 tgaaggatcg tgtgagaatc gcctatccga tggaggattt cgagtacggc atgcgcgagt
     2041 ttgcgatcta cgacaacaac ggctacatgc tgcagttcgg ttcccccgtc cgttcgtgac
     2101 gctgcaggca cggctccgct tcagggccga ctggcgtcag tgagccgctg tatttcttcg
     2161 ccgttcagtg tcagcgtcgc cacctggacg agctccgcga gctgcgcgac cgtccgtgcg
     2221 ctggctattg gcgcgccgac cgttggttga gacagcagcc acgccagcga cacagcggcg
     2281 acagatacct tgtgtgcggc tgccacgtcg tccaggacgc cgaggacgcg ccgtccgcgg
     2341 gcgtcgagat aggcgcgtgc gccttcggcc cgaacgctct cgaccgtggt gcccggccgg
     2401 tacttccctg acaggaatcc ctgcgcgagg gcgaagtagg ggaggcacgc cagaccctcc
     2461 cgcgcgcaga gttcggcaag ggggccctcg tagtcgccgc gatgcaccag gttgtagtgc
     2521 ggctgcagcg cgacatatcg cgacagtccc tcgcgcgtcg agatcgcgag cgcttccgac
     2581 agacgaggcg ccgtgaagtt ggacgccgcg atgtagcgca ccttgccctc gcggacgagg
     2641 ccgtcgaagg cgcggagcgt ctcctcgatc ggtgtgttcg gatcgtcggc gtgcgcgtag
     2701 tacaggtcga tccgatcgct gccgagacgt cgaagcgatg cctctgcggc ggtccggatt
     2761 gttttcggcg acagcccatt caggccggga gcccggccga ccttggtcgc gacaatgacg
     2821 cgattgcggg cgccccgcgc cgccatccag cggccgagga ttgtttccga ctcgccgccg
     2881 cgatggcccg gcacccagaa ggaataggag tccgcggtgt cgatgaagtt gccgcccgcc
     2941 gcggcgtatg catcgagcac ggcaaacgac tcctgctcgc tcgagctcca tccgaagacg
     3001 ttcccaccga ggcagagcgg gaagacgtcc agatccgttc gtccaaggcg cgccatggtt
     3061 a
//
LOCUS       DBNGSH010000928         3360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3855993, whole genome shotgun sequence.
ACCESSION   DBNGSH010000928 DBNGSH010000000
VERSION     DBNGSH010000928.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3855993"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="AC16CY_21250"
     CDS             <1..473
                     /locus_tag="AC16CY_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356393.1"
                     /translation="PDRTVTLVISREWRDEHGVPLKEDFRRTLRVGPPDERPLDPASW
                     RIQAPPAGSRDAFVVTFPEALDHGLLMRALAVRRNGEAIPGDIAVPAGEKQWTFVPNA
                     PWRTGDHELLALDILEDLAGNQIGRAFEVDNFDTVDKSPNPQTITIPFRVRASE"
     gene            561..2903
                     /locus_tag="AC16CY_21255"
     CDS             561..2903
                     /locus_tag="AC16CY_21255"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIR3356394.1"
                     /translation="MRPLFHGLLICLAVSCGLLAQGPAAERSESIRGQVVNTADGVPL
                     RRARVEVTGGRRVDPVFTDDEGRFRVEGAPATSLTVKVTKAGYAPGLVTVPAGGGSTE
                     LSFALTKSAAVMGRVLNRYGGPIRFVTVAGRMILPAADKTQAAVRQFSVQTDELGEYR
                     LGGLPPGRYEIMAGRRLPPGVAPSTPVTDLPRPVEDLLFGSRESLEIAKGALTIALTA
                     GEEVQDVDFTIAGASATCPAASGRMPPVGTASIAGRVTGASGEPLACAIVWPVGPDAP
                     DSSVSTDAQGRYVIEGLHAGSFVLQARKDYRLTLQYGQRHPSDAATPIVLRDKEQRTR
                     VDFVLPHGSIVSGTVVDEHGEPVERTMVTAHRLLRSEGRTAAFTHAPPSSTDDRGQYR
                     LIGLDPGTYIVAATGRGALSGTSPERAQGYVSHYYPGSPDPLYAQRVVLDAGRNADGI
                     DIAFTPTRTFTVSGSVSGPVFEAIGRPFDGNVTLSVSGRSGAVSVDSRGGQVDADGGF
                     VVRNVLPGDYVVKAVASPRGARLFGMQYVTVTDADPPPAAVTVVEGATVEGSWIIEGV
                     QDQDTRRIEISAYTADPDFWPGLPGRPEAQNPVQNDRSSTIPAGMFRIDGVSGPSRFQ
                     VRTPWCEACYVKSMYVNGADVTNTPFDFGVRGGVYRDVEVILSDASATIEGRATDERE
                     APVATSYVVVFSTDRGLWYPASPHVRYERTTTGGAFRVTGLAPGEYFVAAVNRLDDAF
                     PLGLETADPEGLAEQLAQRAERVTLSERDRRTINLRLIRR"
     gene            complement(3043..>3360)
                     /locus_tag="AC16CY_21260"
     CDS             complement(3043..>3360)
                     /locus_tag="AC16CY_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356395.1"
                     /translation="DLLISHELPMADRKRLRLELNIQNLFNQKTATHRYNFLNKGGPA
                     GSQTRASDAIDFTDVNLAAGYDYNALLLASPSGASAYDPRYGMDDLFNPGLQGYFSVK
                     FIF"
BASE COUNT          555 a         1094 c         1137 g          574 t
ORIGIN      
        1 atcccgatcg caccgtgacg ctcgtcatca gccgcgagtg gcgggacgag cacggagtgc
       61 cgctcaagga ggacttccgc cgcaccttgc gcgtgggccc gcccgacgag cggccgctgg
      121 acccggcgtc gtggcggatt caggcgccgc cggccggcag ccgcgacgcc ttcgtcgtca
      181 cgtttcccga ggcgctcgat cacggcctgc tgatgcgggc gctcgcggtc aggcggaacg
      241 gcgaggcgat cccgggcgac atcgccgtgc cggcgggcga gaaacagtgg acgttcgtgc
      301 cgaacgctcc gtggcgtacg ggcgatcacg aactgctcgc gctcgacatc ctcgaagacc
      361 tggccggcaa ccagatcggc cgcgcattcg aagtcgacaa ctttgatacg gtagacaaga
      421 gcccaaatcc gcagacgatt acgatcccgt tccgcgtccg cgcgtcggag tagtacgtag
      481 tgtcctgctt cagccggacc gagagcgcag tcgtacgtag tgtccggctt cagccggacc
      541 agaacgatga caggaaaccg atgagacctc tgttccacgg gttgctcatc tgcctcgccg
      601 tttcttgcgg cctcttggcg cagggtcccg ccgctgagcg ctctgaatcg atccggggcc
      661 aggtcgtcaa tacggcggac ggagtcccgc tgcggcgcgc acgcgtcgag gtgacggggg
      721 ggcgccgagt ggacccggtc ttcaccgacg acgagggacg gttccgcgtc gagggtgcgc
      781 ccgcgacatc cctgaccgtc aaggtcacga aggcgggata cgcccccggg ctcgtgaccg
      841 tgcccgccgg gggcggcagc actgagcttt ccttcgcgtt gacaaagagt gcggccgtga
      901 tggggcgcgt gctcaatcgg tatggcggcc cgattcgatt cgtgaccgtt gccggacgga
      961 tgattctgcc ggctgcggac aagacgcagg ctgcagtgcg ccagttctcc gtgcagacgg
     1021 acgagctggg cgagtaccgc cttggcggcc tgccgcccgg ccgctacgaa atcatggcgg
     1081 ggcgtcgttt accgccgggt gtggcgccga gtacgccagt cacggacctg ccccggccgg
     1141 tcgaagacct gctcttcgga tcgcgggaat cgctcgagat cgcaaaaggt gcgttgacga
     1201 ttgcgctgac agcgggcgag gaggtacagg acgtcgattt cacaattgcg ggcgcgtctg
     1261 cgacctgtcc ggccgcgtca ggtcgcatgc ctcccgtagg cacggcctcg atcgcggggc
     1321 gtgtcaccgg cgcatccggc gaaccgctgg cgtgcgccat cgtgtggccg gtgggcccgg
     1381 acgcgcccga ttcgagcgtg tccaccgatg cgcagggccg ctacgtgatt gaaggtcttc
     1441 acgctgggtc tttcgtcctc caagcccgaa aggactatcg ccttacgctc cagtacggtc
     1501 agcggcatcc ctcggacgcg gcaacgccga tcgtccttcg cgacaaagaa cagcggacac
     1561 gcgtcgactt cgtccttccg cacggcagca tcgtcagcgg aaccgtcgtg gacgagcacg
     1621 gtgagcctgt cgaacggacg atggtgacgg cgcacaggct tctccggtcc gaagggcgga
     1681 cagcggcgtt tacccacgcg ccgccatcgt cgacggacga ccgcggacag tatcggctga
     1741 tcggattgga tcccgggacc tacatcgtcg ctgcgacggg acggggtgcg ttgtcgggaa
     1801 ccagccctga gcgggcgcag gggtacgtgt cccattacta cccgggctcg cccgatccgc
     1861 tgtacgcgca gcgcgtcgtg ctcgatgccg gccgcaacgc ggacggcatc gacatcgcat
     1921 tcacccccac acgcacgttc acggtgtctg gttcggtgtc cggcccggta ttcgaggcga
     1981 ttggcagacc gttcgacggc aacgtcacgt tgtccgtcag cggcagatcc ggcgccgtgt
     2041 ccgtggacag ccggggtgga caggtcgacg ccgacggcgg cttcgtcgtg cgcaacgtgc
     2101 tgccaggcga ttacgtcgtc aaggcggtcg cctcgccccg cggcgcgcga ctgttcggca
     2161 tgcagtacgt gacagtgacc gacgctgacc cgccgcccgc ggcggtcact gttgtcgagg
     2221 gcgcgaccgt tgaaggcagc tggatcatcg agggggtgca agaccaggac accaggagaa
     2281 tcgagatatc cgcgtatact gccgatcccg atttctggcc aggcctgcca ggccggccgg
     2341 aagcccaaaa tcctgttcaa aacgatcgat cttccacgat cccggccggc atgttccgca
     2401 tcgatggcgt aagcggaccg agccgattcc aggtcaggac gccgtggtgc gaggcctgtt
     2461 acgtgaagtc gatgtatgtg aacggggccg acgtcaccaa tacgccgttc gacttcggcg
     2521 tcaggggcgg tgtctatcgc gacgtcgaag tcatcctgtc cgacgctagc gccacgatcg
     2581 aggggcgtgc gactgacgag cgcgaagcgc cagtggccac gtcctatgtt gtcgtcttct
     2641 cgaccgaccg tggcctgtgg tacccggcgt ctcctcatgt gaggtacgaa cgtaccacca
     2701 ccgggggcgc gtttcgggtg accgggctgg ctcctggcga gtatttcgtg gccgccgtca
     2761 atcgccttga cgacgcgttt ccgctcggac tcgagacggc cgatcccgag gggctcgccg
     2821 agcaactcgc gcagcgagct gagcgagtca cgttgtccga gcgcgaccgg cggacgatca
     2881 atctgcgatt gatccgccgg tagcgctcac gacaccacga cggtccggct gaagccggac
     2941 actacgtacg actgcgctcg cggtccggct aaagccggac actacgtacg actgcgctcg
     3001 cggtccggct gaagccggac actacgtacg agtcgcgcgc gatcagaaga tgaatttcac
     3061 gctgaaatac ccctgcaggc cggggttgaa cagatcgtcc atcccgtacc gcggatcgta
     3121 cgccgacgcg ccgctcggcg acgccaggag cagcgcgttg tagtcgtagc ccgccgccag
     3181 gttcacgtcc gtgaagtcga tcgcatccga tgccctggtc tggctgcccg ccggccctcc
     3241 cttgttcagg aagttgtagc ggtgcgtggc cgtcttctgg ttgaacaggt tctggatgtt
     3301 cagctcgagc cggagccgct tccggtccgc catcggcagc tcgtgcgaga tcaggaggtc
//
LOCUS       DBNGSH010000929        13958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3856487, whole genome shotgun sequence.
ACCESSION   DBNGSH010000929 DBNGSH010000000
VERSION     DBNGSH010000929.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 13958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13958
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3856487"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="AC16CY_21265"
     CDS             <1..519
                     /locus_tag="AC16CY_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR3356396.1"
                     /translation="YSRQYELGVDFITSGEYRTLAAAYREIQDIKFPVVVSSGEPADD
                     ADGETVDEGVSGGADATAPAAAPAAPAPPSRARKEPDVKLDSLDAFVEFFITSGKKGV
                     AVNRYKGLGEMNPETLWTTTMDPDNRTLLQVRAEDHAEADQMFTTLMGDQVEPRRKFI
                     EDNALDVRNLDV"
     gene            529..2994
                     /gene="gyrA"
                     /locus_tag="AC16CY_21270"
     CDS             529..2994
                     /gene="gyrA"
                     /locus_tag="AC16CY_21270"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3356397.1"
                     /translation="MSAPASPNRIPVNIEDEMKRSYMDYAMSVIIGRALPDVRDGLKP
                     AHRRVLFAMRQMGLASNRAYRKCAKIVGEVIGNYHPHGDVPAYDTLVRLAQDFNMRCP
                     LVDGQGNFGSIDGDPPAAYRYTEARLEGLAEAMMVDLDKETVDFVPNFDETTDEPTVF
                     PAPFPNLLVNGSAGIAVGMATNIPPHNMREVIEAAIWLITSAQSDDPPTLPEKQRKLL
                     ELVPGPDFPTGGYIVGRQGVQQAYLTGRGTVIMRGKAEIEVSKKGDRSSIVIDAIPYQ
                     VNKARLIERIAELVREKVLEGISDIRDESDRDGMRIVVDLKRGEIGDVILNNLYKHTQ
                     LQSTFGVITLAIVGGRPKVLSLLDVLENFVEFRREIVRRRTEFELRKAEARRHILEGL
                     KIALDHLDAVIALIRASANPAAAREGLMTQFGLTQVQSQAILDMQLQRLTGLERDKIL
                     LELAELLKLIERLLAILGSDTLVMQIAVDELRDVQAKYGEDRRTEILADEGEFRIEDL
                     IAEEDMAITVSNTGYIKRTAISTYRNQRRGGKGRIGMRTREEDFVSHLFVASSHAYIM
                     IFSDRGRAYWLKVHEIPDVGPDGRGKAIANLVSMEEGERIAALLAVKEFEADKYVVMG
                     TRRGVIKKTELSAFSNPRAGGIIAMGVEQGDSVIAVGISDGKNEIFIGTRSGMAIRFP
                     EDDVRPMGRTAYGVRGITLRDDDEVVGMDVLAAGGTILSVTEQGYGKRTGLDEYRVQA
                     RGGLGIINIQTSERNGKVIGVVQVSDDDELMLITQQGKILRMASNNIRSIGRATQGVR
                     LIEIEGEDRAVSIARLAEREGDE"
     gene            complement(2997..5243)
                     /locus_tag="AC16CY_21275"
     CDS             complement(2997..5243)
                     /locus_tag="AC16CY_21275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3356398.1"
                     /translation="MDLLSSLNPEQRQAVLATEGPLLILAGAGSGKTRVIVHRIAHLV
                     SEGLSEPDRILAVTFTNKAAGEMRARVESLLGVDCQGMWLSTFHALCARLLRREAPHI
                     GLSRDFVIYDSSDQLTVMKQVLREAGLDGTLQPRVALSRISHAKNRMEPAEGSEGAWN
                     PRDEQLGQLHAMYARALKEANALDFDDLLLKTVELFDEAPLVRERYGKKFRFVLVDEY
                     QDTNRPQYLLIRHLAPHHNLCVVGDPDQSIYKWRGADLRNILDFEHDFPEAATVRLER
                     NYRSTQVILDAASAVIRHNRNRKDKRLYTERQGGSKIVYYRAGDDLDEAEFVARTSRA
                     ALQESTEDTVAILYRTNAQSRTLEDALRRAGIAYRIIGGVRFYERKEIKDALAYLRLL
                     VNPHDDVSLRRVINVPARGIGKGVMESLDGVVLPSSGDGPPLLSQLAPAAANNSLWAR
                     LLLAIEQRLLPARSLASLTAFRDLLIDLTGMAAEESVSIALGKVLDRSGYLRDLRDER
                     SEDAEGRIENLLELVSAAREYESRSPEPSLAGFVDQLSLLSDVDEETGSKNARVLMLT
                     LHSAKGLEFPVVVISGLEDGLFPHSRATSDEVDLEEERRLCYVGITRAQRRLVLTSAA
                     RRRVFGDYQPTEPSRFLSEIPPELIEDAPSLYVAPQPSYPAFGSRRRRGGPGGYVREE
                     PQAYEYENEDQSVPFGLRLGLRVRHPTFGVGTILRVEPLDDDAKLVVRFAIGQKTLRA
                     KFSKLEMA"
     gene            complement(5310..7154)
                     /gene="glmS"
                     /locus_tag="AC16CY_21280"
     CDS             complement(5310..7154)
                     /gene="glmS"
                     /locus_tag="AC16CY_21280"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR3356399.1"
                     /translation="MCGIIGYLGSKPVVPVLLDGLRRMEYRGYDSAGVALVSPEGIAL
                     RRSAGKLVNLESVLREDPADGVYGIGHTRWATHGRPTEENAHPHRDCTGRIVVVHNGI
                     IENYMELKQELRAQGHEFKTETDTEIVAHLVEREMRSDGLEQAVRRSLKLMRGMFALV
                     LVSVEDPEKIVAVRNGPPIIVGLGEDEFFVASDIPAILSHTRDVVFLGDEEMAVITRA
                     GVEFTDFSGRAVSKTTKRVLWDPIAAEKGGHKHFMLKEIFEQPTAARDTILGRVSLDR
                     GQIFLEDMNLSEDTLRAVLKVTIIACGTSWHAGLVGKNLIETLAKVPVEVDYGSEYRY
                     RSPIVSENELAVVITQSGETADTLAALREAKRKGAASIAICNVVGSMATREADGTIYT
                     HAGPEIGVASTKAFTSQLVALHLLALRLAQVRGTIAPAEMRTCIGELLQIPHVIDDAI
                     RASAAMERVAERFHSRRDFLFLGRGINYPIALEGALKLKEVSYIHAEGYPAGEMKHGP
                     IALIDENMPVVALAPDDAVFEKMQGNVQEAKARGGSVIAITSAGDARMDAVLSSSHDV
                     VVPMPRTTSLLTPIVLTIPLQLLAYYIAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(7156..8565)
                     /gene="glmU"
                     /locus_tag="AC16CY_21285"
     CDS             complement(7156..8565)
                     /gene="glmU"
                     /locus_tag="AC16CY_21285"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR3356400.1"
                     /translation="MPSPDVHVVVLAAGQGVRMRSRLPKVLHAVNGRPMLDRVLRVAE
                     SLSPKTLTVVVGHGADAIRARFTELPRVRFALQEPQLGTAHALLQAAPLLDRQTGALV
                     LLSGDVPLLKAVTLQGLLATHCGSRAAATVVTATVERPSGYGRIVREGGRIARIVEER
                     DASPTERGIPEINSGIYAFDLVPLFEALRGIAAENDQKEYYLTDLIALYRRRKMVVET
                     FLAGDPQEIRGVNSRSELAEANRLARLEKNEEVMAAGVTLVDPAATYIGPDVEIGTDT
                     VIHPGVFLEGNTRVGASCEIHAHVRISDTEIGDRATIKSFTLITRSRVGPGAEVGPFA
                     HLRPESVVGENAKVGNFVELKNTSLGAGSKANHLSYLGDATIGTGVNVGAGTITCNYD
                     GERKHATVIEDGAFIGSDTQLIAPVRVGKGAYVAAGSSITENVPDGALGIARGRQTNV
                     DRWVERRKDATKKDKEAGT"
     gene            complement(8613..9467)
                     /gene="rsmI"
                     /locus_tag="AC16CY_21290"
     CDS             complement(8613..9467)
                     /gene="rsmI"
                     /locus_tag="AC16CY_21290"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR3356401.1"
                     /translation="MSGTLFVVATPIGNLEDVTLRALRVLREVDLIAAEDTRRTSNLL
                     ARHAIRTPLLSFHQHNVRSRTPHLLSRLQSGRTVALVTDAGTPGISDPGVELVKACLE
                     AQIPVESIPGATAPLAAAVLSGFPLIPMTILGFPPTRLKDRTGWFQESSRIRHTFTFF
                     ESPQRVARTMKELVPYLGLRPIVVARELTKVHQSLIRGTAPDIFEQLQQVKGEVTVVI
                     GPEIARDMSNDQFVEPARAAAVAYFGQLAEGGATSRRQAVSLAARRFGLSAKTVYQAV
                     EQAKGSAK"
     gene            9551..11209
                     /locus_tag="AC16CY_21295"
     CDS             9551..11209
                     /locus_tag="AC16CY_21295"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR3356402.1"
                     /translation="MYPSEPFPHFVDDYLAYLHEVLPSYAGLDGVHVHDDLLEDLSRQ
                     ALDTHLRTLSGFLRRLNQIDPSHLSKVERVDHAILVAGIEARMYDLEVVRPWERNPRI
                     YAELIGSSLAGQALFAYAPEAERARRVVSKLRQVPRLVQAARDNVKECPGIFVKVGLE
                     TWRGTLKFIEVDLPRAFSTLDDLHILGDLADTCGEAAAAVRSYIGYLEEDLAPKAKAS
                     FRLGRDRFEQKLRLEDGVKVDSERLLAIALREIESVREEFRSVAGRLNGGDPIEAWRR
                     AKEDHPAAGQLIGVAEAQVGELEEFLRRQDVVSLPDAEPVVVAPSPDFYRWAFASMWT
                     PGPFESKRSQAYYYLTDVDRSWPEDRQTEHLRDFNIPTLWNISIHEVYPGHFLHFRHL
                     QHVTSKVRKSTLFAPASFVEGWAHYCEQMMIEAGFRKGDVTIRLGQLAEALVRLARFV
                     VAIRLHCEDLSVEQGMRIFRDEAFLEEATARREAERGTFDPTYLVYSLGKLMMLKLRR
                     DYKEQYGGRYTLRSFHDAVLSQGSAPFWAIRQLLLDETVDASLE"
     gene            11212..11523
                     /locus_tag="AC16CY_21300"
     CDS             11212..11523
                     /locus_tag="AC16CY_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3356403.1"
                     /translation="MPLYEYECEACGQRFELIRKFSDPEVDTCARCGKGPVRRLFSSP
                     AIQFKGTGWYITDYSQKGKSGTDASAPSSSTDSKSASSTEATPAKKETPAPAAKPASD
                     T"
     gene            complement(11556..12458)
                     /gene="galU"
                     /locus_tag="AC16CY_21305"
     CDS             complement(11556..12458)
                     /gene="galU"
                     /locus_tag="AC16CY_21305"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR3356404.1"
                     /translation="MERPVRTAVFPVAGLGTRFLPATKVLPKEMLVLVDRPIIQYGVE
                     EARLSGIENIVLVTGRGKGAIEDHFDIAYELESLLERRGKTQDLETVRRISRSINVAS
                     VRQGEPLGLGHAVLSARHVVGEQPFAVVLTDDVIDAETPALQQMMDVYARVGGPVIAV
                     ERVPLENVSSYGIVAVDERANLGRGVYRVTDLVEKPRREDAPSTLAIVGRYLLTPDIF
                     PALQQTASDRTGEIQLTNGLRRLLSDRPLYACEIAGVRHDTGNRLGFLKATAYFALRH
                     PELRDSFRQYLETLDVQLGPPASR"
     gene            12492..13778
                     /gene="serS"
                     /locus_tag="AC16CY_21310"
     CDS             12492..13778
                     /gene="serS"
                     /locus_tag="AC16CY_21310"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3356405.1"
                     /translation="MLDPALLRDDLAAVRAGLQKRGADLTKELEDLAVLEARRRRILP
                     ELEGLKREQNTAGDEVARAKRQGLDIGKIQEAGRQRATQVKQLSIELDQVEQRRNRGL
                     LMIPNLPHETVPVGKSSDDNVEVRRHGTPRTFAFTAQPHWDLGPALGIIDFERGTKIA
                     GARFTALVGAGARLERALINFMLHLHTTGHGYVEVAPPFMANGASLTGTGNLPKFEED
                     LFKIAGDWDLYMVPTAEVPLTNMHRGEILDSRQLPIRYTAYTPCFRSEAGSYGADVRG
                     LIRQHQFDKVELMAFSEPEKSYEELERLTLNAEDVLKRLELPYRTVVLCAGDMGFASA
                     KTYDIEVWLPGQNAYREISSCSNTESFQARRANIKYRPQSTGKAEFVHTLNGSGLAVG
                     RTLIAILENYQQADGSVVIPDALRPFMDGASVISAH"
     gene            13839..13928
                     /locus_tag="AC16CY_21315"
     tRNA            13839..13928
                     /locus_tag="AC16CY_21315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13873..13875,aa:Ser,seq:tga)
BASE COUNT         2527 a         4466 c         4528 g         2437 t
ORIGIN      
        1 tattcgcggc agtacgagct gggcgtcgac ttcatcacgt ccggcgagta ccgcacgctg
       61 gccgcggcgt accgcgagat ccaggacatc aagtttccgg tcgtcgtgtc gtccggagag
      121 ccggcagacg acgcggacgg cgagacggtg gacgaaggcg tgtcgggagg agcggacgcg
      181 acagctcccg cagccgctcc agcggcgccg gcgccgccat cgagggcccg caaggagccg
      241 gatgtgaagc tggattcgct tgacgcgttc gtcgagtttt tcatcacgtc gggcaagaag
      301 ggtgtggccg tcaatcgcta caagggtctg ggcgagatga accccgagac gctctggacg
      361 accacgatgg acccggacaa ccgcacgctc ctccaggtcc gcgcggagga ccacgcggag
      421 gccgaccaga tgttcacgac gttgatgggc gaccaggtcg aaccacgccg caagttcatc
      481 gaagacaacg ctctcgatgt ccggaacctg gacgtctagc ctgagtgcat gagcgcacca
      541 gccagcccga atcgcatccc ggtcaacatc gaagacgaga tgaaacgctc gtacatggat
      601 tacgccatga gcgtaatcat tggacgagct ctgccggacg tacgcgacgg tttgaagccg
      661 gcacaccgcc gcgtcctctt cgcgatgcgc cagatggggc ttgcgtcaaa cagggcgtat
      721 cgcaagtgcg caaagatcgt cggcgaggtc atcggcaact accatccgca cggcgacgtt
      781 cccgcctacg acacgctcgt gcgtctcgcc caggacttca acatgcgctg ccccttggtc
      841 gacggccagg ggaacttcgg atcgatcgac ggagatcccc cggcggcgta ccgctacacc
      901 gaggcgcgac tcgaagggct ggccgaagcc atgatggtcg acctcgacaa ggagacggtc
      961 gacttcgtcc ccaacttcga tgaaaccacc gacgaaccaa cggtctttcc ggccccgttc
     1021 cccaacctgc tcgtcaacgg gtctgccggg attgccgtcg ggatggcgac caacatcccg
     1081 ccgcacaaca tgcgggaggt catcgaagcg gcgatctggc tgatcacgag cgcgcagtcc
     1141 gacgatccgc cgacgctgcc ggagaagcag cggaagcttc tggagctcgt tcccgggccc
     1201 gacttcccga cgggaggcta catcgtcggc cgacagggag tgcagcaggc ctacctgacc
     1261 ggtcgcggca ccgtcatcat gcggggcaag gcggaaatcg aggtctcgaa gaagggtgac
     1321 cgctcgtcga tcgtcatcga cgcgattccc tatcaggtca acaaggcgag gctgatcgaa
     1381 cgcatcgcgg agctcgttcg agagaaagtc ctggagggga tttccgacat tcgcgacgaa
     1441 tcggaccgcg acgggatgcg aattgtcgtc gatctcaagc ggggagaaat cggcgacgtc
     1501 attctcaaca acctctacaa gcacacgcag cttcagagca cgttcggcgt gatcacgctg
     1561 gccatcgtcg gcggccgccc gaaggtgctg tcgctgctcg acgtcctcga gaacttcgtc
     1621 gaattccgcc gggaaatcgt caggcggcgc acggagttcg agcttcgaaa ggccgaagcc
     1681 cggcggcaca tcctcgaagg cctcaagatc gccctcgatc atctggatgc ggtgatcgcc
     1741 ctgatccggg cctcggccaa tcccgccgcc gcccgcgagg ggttgatgac ccagtttggt
     1801 ctcacgcagg tgcagtcgca ggcgatcctc gacatgcaac tgcagcgcct gaccgggctc
     1861 gagcgggaca agatcctgtt ggaactggcc gaactgctca agctgatcga gcgcctgctt
     1921 gcgattctcg gcagcgacac gctcgtcatg cagatcgcgg tggacgagct tcgcgatgtc
     1981 caggcgaaat acggcgagga ccgccggacc gagatactgg ccgacgaggg ggagttccgg
     2041 atcgaagatc tgatcgctga agaagacatg gcgatcaccg tgagcaacac cggctacatc
     2101 aagcggacag cgatctccac ctatcgaaat cagcgcaggg gcggcaaggg ccgcatcggg
     2161 atgcggacga gggaagagga cttcgtcagc catctcttcg tggcgtccag ccacgcctac
     2221 atcatgattt tctcggatcg cggccgcgca tactggctca aggttcacga aatccccgac
     2281 gtggggcctg acggccgcgg gaaggccatt gccaacctgg tgtcgatgga ggaaggggag
     2341 cgaatcgcgg cgcttctggc cgtcaaggag ttcgaggcgg acaagtacgt tgtcatgggg
     2401 acccgccgag gcgtcatcaa gaagacggag ttgagcgcgt tcagcaatcc acgggccggc
     2461 ggcatcattg cgatgggcgt cgagcaagga gactcggtca tcgcggtcgg aatctcggac
     2521 ggcaagaacg agatcttcat cggcacacgc agcggcatgg caatccgctt cccggaggac
     2581 gacgtccggc cgatgggccg gacggcttac ggcgtgcgcg gaatcacgct tcgggacgac
     2641 gacgaagtcg tcgggatgga cgtgctggcg gccggcggga ccatcctgtc ggtcaccgag
     2701 cagggctatg ggaagcggac cggactcgac gaataccgcg tccaggcgcg aggcggcctg
     2761 gggatcatca acatccagac cagtgagcgc aatggcaagg tcattggcgt ggtgcaagtc
     2821 agcgacgacg atgagttgat gctgatcacg cagcagggca agatcctgcg gatggcgtcg
     2881 aacaacatcc gcagcatcgg ccgcgccacg cagggcgtgc gcctcataga gatcgagggc
     2941 gaggatcgcg ccgtttccat cgcccgcctc gccgaacggg aaggcgacga gtagggctac
     3001 gccatctcga gtttcgaaaa cttggctcgc agcgtcttct gcccgattgc gaaccgcaca
     3061 acgagcttcg cgtcgtcgtc gagcggctcg acccgcagga tggtgccgac gccgaatgtc
     3121 ggatgtcgca cccgtagacc gagccgcagt ccaaacggca ccgactgatc ttcgttttcg
     3181 tattcgtagg cctgcggttc ttccctgaca tacccgcccg ggccgcctcg ccgccgcctg
     3241 gagccgaagg ccggatacga cggttgcggc gccacgtaca acgaaggcgc gtcctcgatc
     3301 agctcgggcg gaatctcgct caggaagcgg gacggctcgg tcggctggta atcgccaaag
     3361 acacgccggc gagcagcaga cgtcagcacg agccggcgct gtgcccgcgt gattccgacg
     3421 tagcacaggc ggcgctcctc ttcgagatcg acttcatccg acgtcgctct ggagtgggga
     3481 aaaaggccgt cctcgagccc gctgatcacc accaccggaa actccaggcc cttggcgctg
     3541 tgcagggtca gcatgaggac gcgcgcgttt ttcgagcccg tctcctcgtc cacgtccgag
     3601 aggagggaaa gctggtcgac gaagccagcc agcgacggct cggggcttcg gctctcgtac
     3661 tcccgcgcgg ccgacacgag ctccagcagg ttttcaatcc ggccctcggc gtcctcgctg
     3721 cgctcgtcac ggaggtcccg gaggtatccg gagcgatcga ggacctttcc aagggcgatc
     3781 gagaccgatt cctcggccgc catgccggtc aggtcgatca gcagatcgcg gaacgccgtc
     3841 agcgacgcga gcgaccgggc cggcagcagg cgctgctcga tggcgagcag aagccgggcc
     3901 cacagcgaat tgttggctgc tgccggggcc aactggctga gaagcggcgg cccgtccccg
     3961 ctcgacggca gaaccacgcc gtcgagagac tccatcacgc ccttcccgat cccccgtgcg
     4021 ggcacgttga tgacacgccg gaggctcacg tcgtcgtgag ggttcacgag gagcctcagg
     4081 taggccagcg cgtctttgat ttcctttcgc tcgtagaacc ggacaccgcc gatgattcgg
     4141 tacgcgatgc cggcgcggcg cagcgcgtct tcaagcgttc gcgactgtgc gttcgtacga
     4201 tacaggatgg cgacggtgtc ttccgtgctt tcctggagtg cggcgcgcga ggtgcgcgcg
     4261 acgaactcgg cctcgtcgag gtcgtcgccc gcgcggtaat acacgatctt gctgccgccc
     4321 tgacgctccg tgtacaggcg cttgtccttc cggttccggt tgtgccggat gactgcggac
     4381 gcggcatcga gaatgacctg ggtcgagcgg taattgcgct cgaggcgcac ggtggctgcc
     4441 tcggggaagt catgctcgaa gtcgaggatg ttgcgcagat cggcgccgcg ccacttgtag
     4501 atcgactggt cggggtcgcc gacgacacag aggttgtggt gcggggcgag gtgccggatc
     4561 agcaggtact gcggccggtt ggtgtcctgg tattcgtcga ccagcacgaa gcggaacttt
     4621 tttccgtacc gctcccgtac gagcggcgcc tcgtcgaata gctcgaccgt cttgagcagg
     4681 agatcgtcga agtcgagggc gttggcctcc ttgagcgccc gtgcgtacat cgcgtggagc
     4741 tggcccagtt gctcgtcgcg agggttccag gcgccctcgc tgccttcggc cggctccatg
     4801 cggttcttgg cgtggctgat tcgcgacagc gcgacccgcg gctgaagcgt tccgtccaat
     4861 ccagcctcgc gcaggacctg cttcatgacc gtgagctggt cgctcgagtc gtagatgacg
     4921 aagtccctcg acagcccgat gtggggcgcc tcccgccgga gcaacctggc gcacagcgcg
     4981 tggaacgtcg acagccacat gccctggcag tcgacgccga gaagcgactc gacgcgggcc
     5041 cgcatctcac cggcggcctt gttggtgaag gtgacagcca gaatccgatc tggttccgac
     5101 agaccctcgc tcaccaggtg ggcgatgcgg tgcacgatga cgcgcgtctt gccggagccc
     5161 gctccggcaa gaatcagcag cggcccttcg gtcgcgagaa cggcctgccg ctgttcaggg
     5221 ttgaggctgg acaggagatc cacgggacga ttcacggtcg acacttccac ccacagtgtc
     5281 ccgtcgtccg atggacggag tcaatgcgtc tattccacgg tgacgctctt ggcgaggttg
     5341 cgaggctgat cgacgtcgca gccgcggcgc accgcgatgt agtaggcgag caactgcaac
     5401 gggatcgtga gcacgatcgg cgtcaacaag gacgtggttc gcggcatcgg gacgacgacg
     5461 tcatgagagg aggacagcac ggcgtccatg cgcgcgtctc ccgcgctcgt gatggcaatg
     5521 acggatccgc cgcgcgcctt ggcttcctgc acgttgccct gcatcttctc gaagacggcg
     5581 tcgtcggggg cgagcgcgac aaccggcatg ttctcgtcga tgagcgcgat cggtccgtgt
     5641 ttcatctctc ccgcggggta tccctccgcg tggatgtagg agacttcctt gagcttgagc
     5701 gcgccctcga gcgcgatcgg atagttgatg ccccgcccca ggaacaggaa atcgcggcgc
     5761 gagtggaacc gctcggccac gcgctccatc gccgccgagg ccctgatcgc gtcgtcgatg
     5821 acgtggggga tctgcaggag ctcgccgatg cacgtgcgca tctcggccgg cgcgatcgtg
     5881 cctcgaacct gggccaggcg cagggcgaga aggtggagcg cgacgagctg cgaggtgaac
     5941 gccttcgtcg aagcgacccc gatttcgggg ccggcgtggg tgtagatcgt gccgtccgcc
     6001 tcacgcgtcg ccatgctccc gacgacgttg cagatggcga tgctcgcggc gcccttgcgt
     6061 ttcgcttccc gaagcgcggc cagcgtgtcg gccgtctcgc cagactgcgt gatcacgacc
     6121 gccagctcgt tctctgacac gatcggcgac cggtagcggt actccgaccc gtagtccacc
     6181 tccacgggca cctttgccag cgtctcgatc aggttcttgc caacgaggcc ggcgtgccac
     6241 gacgtcccgc acgcgatgat cgtcactttg agcaccgccc gcagcgtgtc ttccgacaga
     6301 ttcatgtcct cgaggaagat ctggccgcgg tccagtgaga cacggcccag gatcgtgtcg
     6361 cgcgcggccg tgggctgctc gaagatctcc ttgagcatga agtgcttgtg cccgcccttt
     6421 tcagccgcga tcgggtccca cagcacgcgc ttcgtggttt tcgataccgc tcgacccgaa
     6481 aagtcggtga actccacgcc ggcccgcgtg atgacggcca tttcctcgtc gcccaggaag
     6541 acgacgtccc tcgtgtggct caggatggcg ggaatgtcgg atgcgacgaa gaactcgtcc
     6601 tctcccagcc ccacgatgat cggcgggccg ttacggacag ctacgatttt ctccgggtcc
     6661 tccaccgaga ccagcaccag ggcaaacatg ccgcgcatca gcttcagcga gcgccggacg
     6721 gcctgttcaa ggccgtcgga gcgcatctcc cgctcgacca ggtgggcgac gatctccgtg
     6781 tcggtctccg tcttgaactc gtggccctgt gcccggagct cctgtttgag ctccatgtag
     6841 ttctcgatga tgccgttgtg gacgacgacg attcggccgg tgcagtcccg gtgggggtgt
     6901 gcattctctt ccgtggggcg cccgtgagtg gcccaccgcg tgtggccgat gccgtagacg
     6961 ccgtcggcgg gatcctcgcg cagaacgctt tcgagattca ccagcttccc ggccgaacga
     7021 cggagcgcga tcccctcagg gctcaccagg gcgacgcccg cggagtcgta tccccgatat
     7081 tccatccgcc gcaatccgtc cagcagcacg ggtacgaccg gtttcgagcc gaggtagcca
     7141 atgattccgc acatatcagg ttcctgcttc cttgtccttt ttcgttgcgt ctttccggcg
     7201 ttcaacccaa cgatcgacgt tggtctgccg gccgcgcgcg atgcccagcg ctccgtccgg
     7261 cacgttctcg gtgatcgacg atccggcggc gacataggcg cccttcccca ctcgcacggg
     7321 ggcgatgagc tgcgtatcgc tgccaatgaa ggcgccgtcc tcgatcacgg tcgcgtgctt
     7381 gcgctcgccg tcgtagttgc acgtgatcgt gcccgccccc acgttgacgc ccgtcccgat
     7441 cgtcgcgtcg cccaggtacg acaggtggtt ggccttcgac ccggcgccga gcgacgtgtt
     7501 cttgagctcg acgaagttgc cgaccttcgc attttcgccg acgaccgact caggccgcag
     7561 gtgggcaaac gggccaacct ccgcgcctgg ccccacgcgg gagcgcgtga tcagcgtgaa
     7621 gctcttgatg gtagcgcggt cgccgatctc ggtgtctgaa atccggacgt gcgcgtggat
     7681 ctcgcaggac gctcccacgc gcgtgttccc ctcgaggaac acgccgggat ggatcaccgt
     7741 gtccgtgcca atctcgacat ccggcccgat ataggtcgca gctggatcga cgagggtgac
     7801 gccagccgcc atgacttcct cgttcttctc gagcctcgcg agcctgttcg cttcggccaa
     7861 ctcgctgcgg ctgttcaccc ctcggatctc ctgcggatcg ccggcaagaa acgtctccac
     7921 caccatcttg cgccgccggt agagggcaat gaggtcggtc aggtagtact ccttctgatc
     7981 gttctcggcc gcgatgccgc ggagagcctc gaacagaggc acgaggtcga acgcgtagat
     8041 gccgctgttg atctccggga tccctcgctc agtcggagac gcgtcgcgct cttccacgat
     8101 tcgcgcaatc cggccgccct ccctcacgat tcgcccgtag ccgctcggcc gttcgaccgt
     8161 ggccgtgacc actgtcgctg cggctctcga gccacagtgg gtcgcgagca gcccttgcag
     8221 cgtgactgcc ttgaggagtg gaacgtcgcc ggacaacagg acgagagcgc cagtctgtct
     8281 gtccagcaga ggcgccgcct ggagcagcgc atgggcagtg cccagctgcg gctcctggag
     8341 agcgaaccgg acccgaggca actcggtgaa acgcgcccgg atcgcgtcgg cgccgtgtcc
     8401 gacaacgacg gtcaacgtct tgggagacaa cgactccgcg acccgcaaga cgcggtccag
     8461 catcggtcgt ccattgaccg cgtggaggac cttcgggagt cgcgaccgca ttcggactcc
     8521 ctgaccagca gcgagcacga cgacgtggac gtctggggag ggcatggatc gttcaggaaa
     8581 ttaacgcaag gatcgaaccg ggcaccgtct ggtcatttgg cggagccctt ggcctgctcg
     8641 acggcctgat aaacggtctt cgccgaaaga ccaaaccgtc tcgcagccag cgaaacggcc
     8701 tgccttcttg aagtcgcccc accttcagcc aattgaccaa aataggcgac ggccgccgct
     8761 ctcgctggct caacgaattg gtcatttgac atatctctgg caatctctgg tccgatgaca
     8821 accgtgactt cacccttaac ctgttgtaat tgctcgaaaa tgtcgggagc agtcccccga
     8881 atgagcgact gatgcacctt cgtgagctca cgcgccacaa caataggccg taagcccaaa
     8941 tatggcacaa gctccttcat cgttcgggca accctttggg ggctttcgaa gaacgtgaac
     9001 gtatgacgga tcctcgatga ctcttgaaac caaccagttc tgtcttttaa cctagtcggt
     9061 gggaatccca gaattgtcat cggaatcagc ggaaaccctg aaaggaccgc ggccgccagg
     9121 ggggctgtcg ctccgggaat cgactcaacc gggatctggg cctccagaca ggccttgacc
     9181 agctctacgc cagggtcaga gatgcccggg gtgcccgcgt ccgtgacgag cgctactgtt
     9241 cggcccgact gcagccgtga aaggaggtgt ggcgtccgcg aacgaacgtt gtgctggtga
     9301 aaactgagga gaggtgtcct gatcgcgtga cgagccagca agttcgaggt gcggcgtgtg
     9361 tcttccgcgg cgatcagatc aacctctcga aggacccgta acgcccgcag cgtgacgtcc
     9421 tcgaggttgc cgatcggtgt ggcgaccacg aacaacgtgc cggacatcgc ctctggaagt
     9481 ccggttattg taccattgac gggatgagcg ctccccgtgg ggcggcgact tgcacgacca
     9541 ttatcccggc atgtacccat ccgagccgtt cccgcacttc gttgacgact acctcgcgta
     9601 tttgcatgag gtgctgccta gttacgcagg cctggacggc gtccacgtcc acgacgacct
     9661 gctcgaggac ctgagtcgcc aggcgctcga cacccatctg cgcacgctgt ccggattcct
     9721 ccggcggctc aatcagatcg acccgtcgca tctctcgaaa gtcgagcgcg tggaccacgc
     9781 gatcctggta gccggaatcg aagcgcggat gtacgacctc gaggtggtgc ggccctggga
     9841 gcgcaacccg cgaatctacg ccgagctcat cgggagcagt ctcgccggcc aggcgttgtt
     9901 cgcgtacgcg cccgaggccg aacgcgcccg tcgcgtcgtg tcgaagttgc gccaggttcc
     9961 ccgtctcgtc caggccgcgc gcgacaacgt caaggagtgt cccggtatct tcgtgaaggt
    10021 cggtctcgag acctggcggg gaacactgaa gttcatcgaa gtcgatttgc cgcgcgcgtt
    10081 ttcgacgctc gacgacctgc acatcctcgg agacctcgcc gacacgtgcg gcgaagcggc
    10141 agccgccgtc aggtcctata tcgggtatct ggaggaggat ctggcgccga aggccaaggc
    10201 ctcgttccgc ctcggtcgcg accggttcga gcagaaactc aggctggagg acggcgtcaa
    10261 agtcgactcc gagcgactgc ttgcgatcgc gctccgggag attgaatccg tccgcgaaga
    10321 atttcggtcg gttgcgggac gactgaacgg aggcgacccg atcgaggcgt ggcgacgggc
    10381 gaaggaggac catcccgcgg cgggtcagtt gatcggggtc gccgaggcgc aagttggtga
    10441 gctcgaggaa ttcctgagga gacaggatgt cgtgtcgctc ccggacgccg agccggtcgt
    10501 cgtcgcaccg tcaccggatt tctaccggtg ggcctttgcc agcatgtgga cgccgggtcc
    10561 cttcgagagc aagcgaagcc aggcgtatta ctacctgaca gacgtggatc ggagctggcc
    10621 cgaggatcgg cagaccgagc accttcgcga cttcaacatc ccgaccctct ggaacatctc
    10681 catccacgaa gtctacccgg gccacttcct gcacttccgg cacctgcagc acgtcacgtc
    10741 caaggttcgc aagtcgacgc tcttcgcgcc tgcatcgttt gtcgagggct gggcgcacta
    10801 ctgcgaacag atgatgatcg aagccggctt ccgcaaaggc gacgtgacga ttcgtctcgg
    10861 ccagctggcg gaagccctcg ttcggctggc gcgcttcgtg gttgcgattc gcctgcactg
    10921 tgaggatctc tcggtcgagc aagggatgcg catcttccgc gacgaggcat tcctggagga
    10981 ggcgacggcc cgccgcgagg ccgagcgcgg gaccttcgat cccacctacc tggtgtactc
    11041 gctcggcaag ctgatgatgc tcaagctccg ccgcgactac aaggagcagt acggggggcg
    11101 gtacacgctc cgatcgtttc acgacgcggt gctgtcgcaa gggagcgcgc cgttctgggc
    11161 gatcaggcag ctgttgctcg atgaaacggt ggacgcgtcg ctcgaatagc catgcctctc
    11221 tacgagtacg agtgcgaggc gtgcggacag cgattcgaat tgatccggaa gttctccgat
    11281 cccgaagtgg acacgtgcgc gcgctgtggc aagggccccg tgcggcggtt gttctcgtct
    11341 cccgcgatcc agttcaaggg caccgggtgg tacatcaccg actactcgca aaagggcaag
    11401 agcgggaccg acgcgtctgc gccatccagt agcactgatt ccaagagcgc ctcgtccacc
    11461 gaggcgacgc ccgcgaagaa ggagactcca gcgccggccg ccaagcccgc gtccgatacc
    11521 tgacgtggtt tctgctcagc gggaggagag ccgtttcaac ggcttgccgg cggtccgagc
    11581 tgcacgtcga gtgtttcgag atactgacgg aaggaatcgc gcagctccgg atgccgtagc
    11641 gcgaagtacg ccgtcgcttt caggaacccc agcctgtttc ccgtgtcatg gcggacgccg
    11701 gcaatctcgc acgcgtagag gggccggtcg ctcagcaacc tccgcaaccc gttggtgagc
    11761 tggatttctc ccgtgcgatc gctggcggtc tgctgcagcg ccggaaaaat gtccggcgtc
    11821 agcaggtatc gcccgacgat ggccagcgtc gaaggggcgt cttctcttcg cggtttctcg
    11881 acgagatccg tgacgcggta gacgcctcga ccgagattcg cccgctcgtc gactgccacg
    11941 atgccgtagc tcgagacgtt ttcgagaggc acacgttcga cggcgatgac cgggccgccg
    12001 acgcgagcgt acacgtccat catctgctgg agggccggcg tctccgcgtc gatcacatcg
    12061 tcggtgagga caaccgcaaa aggctgctct cccaccacgt ggcgcgccga cagcacggca
    12121 tggcccagcc ccagcggctc tccctggcgc accgacgcga cgttgatcga ccgcgagatc
    12181 ctgcggaccg tttccaggtc ctgcgtcttg ccgcgacgtt cgagaaggct ctcgagctcg
    12241 tatgcgatgt cgaagtgatc ctcgatcgcg cccttgccgc gaccggtcac caggacgatg
    12301 ttttcgattc ctgacaggcg cgcttcctcg acgccgtact gaatgatcgg ccggtcgacg
    12361 agcacgagca tctctttggg gagcaccttc gttgccggga gaaaacgggt ccccaatccc
    12421 gcgactggaa aaacggccgt tctgacgggt cgctccatgc ccgacatcgt atcgcaggta
    12481 gaatcggcat catgctggat ccagcactgc tccgcgacga cctcgccgcc gtccgcgccg
    12541 ggctgcagaa acgcggcgcc gacctcacga aggagctcga ggatctggcc gtgctcgagg
    12601 cgcgccggcg acggattctt ccggagttgg aggggctcaa gcgcgagcag aacacggcgg
    12661 gtgacgaggt agcccgcgcc aaacgacagg gcctggacat cggcaagatt caggaagccg
    12721 gccggcagag ggcgacgcag gtcaagcagc tcagcatcga gctcgaccag gtcgagcagc
    12781 ggcgcaatcg cgggctgctg atgattccca atctgccgca cgaaaccgtt cccgtcggca
    12841 agagcagtga cgacaacgtg gaggttcgac gccacggaac gccgcgcacg ttcgcgttca
    12901 ccgcgcagcc gcactgggat ctgggccctg cgctcggcat catcgatttc gagcggggga
    12961 ccaaaattgc cggcgctcga ttcaccgcgc tcgtgggagc cggggcgcga ctggagcgcg
    13021 cgctgatcaa cttcatgctc cacctccaca cgaccgggca cggctacgtg gaggtggcgc
    13081 cgccgttcat ggccaacggc gcgtcgttga ccggcaccgg caatctcccg aaattcgagg
    13141 aagacctgtt caagatcgcg ggcgactggg acctgtacat ggtgccgacc gcggaagtgc
    13201 cgctgacgaa catgcaccgc ggcgagatac tggactcgcg ccagctcccg atccggtaca
    13261 cggcatacac gccctgtttc cggagcgagg cggggtcata cggggcggac gtgcgtggcc
    13321 tgattcgaca gcatcagttc gacaaggtcg aactgatggc gttcagcgag cccgagaagt
    13381 cgtacgagga actggagcgc ctgacgctga acgccgagga cgtcctgaag cgtctcgagc
    13441 tgccgtaccg cacggtcgtg ctgtgcgccg gagacatggg ttttgcgtcg gccaagacct
    13501 atgacatcga agtctggctt ccagggcaga acgcgtatcg ggagatctcg tcgtgcagca
    13561 acaccgagtc gtttcaggcg cggcgggcga acatcaagta ccggccgcaa tcgaccggga
    13621 aggcggaatt tgtccacacc ttgaacggct ccgggctcgc cgtcggcagg acgctcatcg
    13681 cgatcctgga gaactaccag caggcggacg ggtccgtcgt gattccggac gcgctgcggc
    13741 cgttcatgga cggggcgtcg gtgatcagcg cacactaact gcggcgggtt caggggtgcg
    13801 acaggttcaa ggggtttgcg ctcgctgcgc ccgcgcacgg agggatggcc gagtggttga
    13861 aggcggcggt cttgaaaacc gttggatcgg aaacggtccc gggggttcga atccctctcc
    13921 ctccgccaac cttcgccaaa cgctcgcttc gctcgcgg
//
LOCUS       DBNGSH010000930         2070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3348255, whole genome shotgun sequence.
ACCESSION   DBNGSH010000930 DBNGSH010000000
VERSION     DBNGSH010000930.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3348255"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="AC16CY_21320"
     CDS             <1..2070
                     /locus_tag="AC16CY_21320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3356406.1"
                     /translation="LDDDVARGGHPVVMLSHGYWQRAFGSDPQVVGRVLRMGRRSYTI
                     IGVAPADYPGGVPVLTPAFYIPMTMLDEVMGFEMLDERFVHNFFVKARLAPGVTRVQA
                     EHAASLVAASLTATRPEGWVPGAKFALVPTSEVQFLPGVDGLLRAATWLLTAVVGLVL
                     LLASTNLASFLLARAIDRGQEVAVRRALGATRGGLARQLLVESVLLGLGGAAAGLALA
                     FALLNVLLWVDLPLSYGMTLDVHFGLDWTMLFEWRVLAIAAGAGVLAGAFLGIIPAVQ
                     GTRADPGAALKTGSRGSDAPRALRWRNVLVVAQVAISLVLLVGAGLFLRGWQQTLAVD
                     PGFGREPTAIVSVMVPVRQSAPDEAVRRTRLLLERFRALTGVEAAGVIQPLPLEFNFS
                     FTDFTIDGRIPPAGQETFRADSAFVDGGFFDAAGMDIVEGRTFNDDDRRDNRPVAVIS
                     RAMARRYWPDGEALGRILRRPDPALPDVEIVGVAGDIMVRSLGEPPRDVIYTPYTQGP
                     GIPMLNFLVRTAGDPARMLPILASAGQEVVPDLRVVQSTTMTKHLSLSRLPSQIAAFL
                     LSAFGVMAVALASVGLYGMVRYTVAMRTREVGIRMALGAEAAVVTRLLAARGLRLVLL
                     GSAIGVAASLLAARFLVTMLYGGNAFDPVAFVGAPLVLGAAAWLAAYLPARRASRVDP
                     LAALRSE"
BASE COUNT          264 a          688 c          749 g          369 t
ORIGIN      
        1 ctggacgacg atgtggcgcg gggcggtcac ccggtggtga tgctcagcca cggatattgg
       61 cagcgcgcct ttggcagcga cccgcaggtg gtcggccgcg tgctgcgcat gggccggcgc
      121 agctacacga tcatcggcgt cgccccggcc gactatccgg gcggcgttcc tgtgctgaca
      181 ccagcgttct acatcccgat gaccatgctg gacgaggtga tgggattcga gatgctcgac
      241 gagcgcttcg tccacaattt cttcgtcaag gcacggcttg cccccggagt gaccagagtc
      301 caggccgaac acgccgcgtc gctcgtggcg gcgtcgctga ccgcgacgcg gcccgagggg
      361 tgggtccccg gcgcgaagtt cgcgctcgtg ccgacgtccg aggtgcaatt cctccccgga
      421 gtggacgggc ttctgcgcgc cgcgacctgg ctgctgacgg ccgtcgtggg actggtgctg
      481 ctgctggcga gcacgaacct tgcgagcttt ctgctggcgc gcgctatcga ccgcggccag
      541 gaggtggccg tgcgccgtgc cctcggcgcg acacgcgggg gactcgcgcg tcaactgctc
      601 gtcgagagcg tgctgctcgg gctcggtggg gcggcggccg gcctggccct ggcgttcgcg
      661 ctcctcaacg tgctgctgtg ggtcgatctt ccgttgtcct atggcatgac gctcgacgtg
      721 cacttcggtc tcgactggac gatgttgttc gagtggagag tgctcgcgat cgcagcaggc
      781 gctggcgtgc tggctggggc attcctcgga atcattccag ccgttcaggg cacgcgcgcc
      841 gatcccggcg cggcgctcaa gacgggaagc cgggggagcg acgctccccg ggcgctcagg
      901 tggcgcaacg tgctcgtggt tgcgcaggtt gccatatcgc tcgtgctgct cgtcggggcc
      961 ggcctgttcc tgaggggctg gcagcagacg ctggccgtgg accccggctt cggccgcgag
     1021 ccgacggcga tcgtttcggt tatggtgccg gtcagacaat cggctccgga cgaggccgtg
     1081 cgtcgtacgc gtctcctgtt ggagcgcttc cgggcactga ccggcgtcga ggccgccggc
     1141 gtcatccagc cgctgccgct cgaattcaac ttcagcttca ccgacttcac gatcgacgga
     1201 cgcatccccc ccgcgggcca ggagaccttc cgcgcggata gcgcgttcgt ggacggcggg
     1261 ttcttcgacg cggccggaat ggacatcgtg gagggccgga cgttcaacga cgacgaccgg
     1321 cgcgataaca ggcctgtcgc ggtgatcagc cgtgcgatgg cccgtcggta ctggcccgac
     1381 ggggaggcat tggggagaat tcttcgccgt cctgaccccg cgttgcccga cgtggagatc
     1441 gtcggcgtag caggcgacat catggtgcga tcgctcggcg agccgccacg cgatgtcatt
     1501 tacacgcctt atacccaggg cccgggcata ccgatgctga atttcctggt ccgcacagcc
     1561 ggtgacccgg cccggatgct gccgatcctc gccagcgccg gacaggaggt cgtgcccgac
     1621 ctgcgcgtgg tgcagtccac gacgatgacg aagcacttga gcctatcgcg cctgccgtcg
     1681 cagatcgccg cgttcctgct ctcggcgttc ggggtgatgg ctgtggcgct ggcctctgtc
     1741 ggactctatg ggatggtgcg gtacaccgtg gcgatgcgga cccgcgaggt cgggatccgc
     1801 atggcgctcg gggccgaggc ggccgtcgtc acgcgactgc tcgcggcgcg cgggctgcgg
     1861 ctggtgctgc tcgggagcgc catcggcgtg gccgcgtcgc tgctcgctgc acgcttcctc
     1921 gtgacgatgc tgtacggcgg caacgcattc gacccggtcg cgttcgtcgg tgcgccgctc
     1981 gtcctcggcg cggcagcgtg gctggccgcg tatctgcccg cgagacgcgc cagccgcgtg
     2041 gacccgctcg ccgcgctccg cagcgagtga
//
LOCUS       DBNGSH010000931         8442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2160459, whole genome shotgun sequence.
ACCESSION   DBNGSH010000931 DBNGSH010000000
VERSION     DBNGSH010000931.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2160459"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="AC16CY_21325"
     CDS             complement(<1..1187)
                     /locus_tag="AC16CY_21325"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3356407.1"
                     /translation="MKSRVVAVLLVSIVGAARLGADTPLVEAARRGDTSAVRALLQAK
                     ADSNVAEPDGTTALHWAVRAGSLPTADLLLRAGARVAAANRYGITPLYLAAMNGDAAM
                     VSRLLEAGADPNAALADGETVLMTAARTGDVATLKALIARGADVRARERRKGQDALMW
                     AAADNNADAVRVLVEAGADRNGKSKAGSFTPLLFAVRGGHIGSTRVLLDLGVDVNQSL
                     SDGTSALVLAVVNAHYELAGFLLERGANPNAEAQGWSALHQIAWSRRHNAGFNLPGPV
                     ATGSLDSLDLARQLVKRGANVNARVTKEPRDGNRNMLNRIGATPFLMAAKNDDVPLMK
                     TLLELGADPSLTTNRGTTALMVAAGVGIWAPGENPGTHEEALAAAKLALEVGGGRIND
                     IDM"
     gene            complement(1184..2554)
                     /locus_tag="AC16CY_21330"
     CDS             complement(1184..2554)
                     /locus_tag="AC16CY_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3356408.1"
                     /translation="MFITRKALPRRTFLRGMGAALGLPLLDAMVPALSATSQTAAKPV
                     RRLGFIYLPNGVARNFTGINYWTPTGEGTGFELSPILSPLAPYKNRLLVISGLAQHQA
                     DAFDDGANGDHTRSVASWLTGVHCKRTEGADVRNGISADQIAAPHLGKDTALPSLELA
                     IDLNFLGGQCENSYSCAYLNTLSWSSATTPLPTENNPRIVFERLFGDGGTNEQRLAQA
                     RRNQSILDSVVADLARLQASLGAGDRAVVADYLDSVREVERRIQAIEARDGAELPTLE
                     RPAGVPDRFDQHVKLMYELQWLAFRADATRVVTFMLGRELNFRTYPEIGITEGHHGLS
                     HHQDNPNQLAKYARLNTYQTELFAWFLDKLQATPEGDGTLLDHSMFLYGASLSNPNLH
                     AHYDLPLAIVGGGAGQIQGGRHLVFRNETPMTNLLLSMLDKVGVHAETLGDSTGRLQV
                     EPLSGV"
     gene            complement(2555..5230)
                     /locus_tag="AC16CY_21335"
     CDS             complement(2555..5230)
                     /locus_tag="AC16CY_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HIR3356409.1"
                     /translation="MGHRGTRRRRIARRAPLLFSTVLALGLAAVWSGRHTQAAPKQAA
                     PAAPEAAHRATLDRYCLTCHTQRLKTQGTVPISLEGLDLARVGDDAQVWEKVVLKLRA
                     GLMPPASSPRPDKATHDALASWLEAELDRASRANVNPGRTEPIHRLNRAEYRNAVRDL
                     LDLDVDVAALLPADDVSYGFDNIAGVLKMSPTLMERYLTAAQKVSRLAIGTPLPVPSI
                     DYYRVADDLSQDEHLPGLPLGSRGGTLVRYTFPMDGVYEFRPRLTRDLNESVPLYAEE
                     QQLEISIDGERVGLFTLPGVSGAGNAQPPEDPDNPSEPPAQPASAPPQRPGGQGQPAR
                     PAISQIQQTVRASAKERASRNRADETWNLRVPVKAGARDVVVTFLNRVSALDETARLP
                     FERPYPAGVNIPETRLGAYLRSVEISGPYSSTGSGNAPSRKRIFVCHPSSSSSGRPAS
                     APDAAAERTCARQILSTLARRAYRRPAASADVDTLMAFFDEGRAQGGFDMGVEHALRR
                     LLASPEFLLRVERDPASAEGSGEARRSAPDAQRRPAGVAGVYRISDIELASRLSFFLW
                     SSIPDDQLLDLAVNNRLGDAAVLTAQVRRMIADPRADAFVKNFAGQWLFLRNLDAVVP
                     VQSIFPDFDDALRQGFRRETELFFDSIVREDRSALDLLRADYTFVNERLAEHYGIPNV
                     KGNHFRRVTLGSDTKRRGLLGQGSILTVTSYPDRTSPVVRGKWILENLLGSPPPPPLP
                     NVPQLRATTAEGQVLSMRQRMERHRANPVCASCHAMMDPLGLSLENFDAIGRWRTLGE
                     SAAPIDVSGVAPDGMKFEGADGLRAALLRSDRFVATLTEKMMTYALGRGLEYYDAPAV
                     RAILRDGSRDEYRFSTLVLGVVQSAPFRMRKAAGG"
     gene            5392..6930
                     /locus_tag="AC16CY_21340"
     CDS             5392..6930
                     /locus_tag="AC16CY_21340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR3356410.1"
                     /translation="MHESGVHYRQLVDLSPDGILVSRDNRIVFVNPAAMRLLGAVSPD
                     RILGQPPLRFFPPDRHAHLRERFRQLAMGKAVPPAEETLVGITGVTTDVELASSLFED
                     PPDRAIQLIVRDISERKRTESALRESEERLSLAFAGAQEGVWDWNLETGAVVYSSHWK
                     QMLGYTEDEIEPHVSAWERLLHAEDKPRTQEVFDGVVRGERNYTMEFRLRHKAGHYVH
                     VLSRGFPVRREDGRVVRIVGTHFDLTERKQAEQALRRAQEELELRVRERTGELALANV
                     SLRAEMAERERAERARTELLSRLVFAQEDERRRIAREMHDQFGEQLTALGLGIRALKR
                     AFEDHPALRAQVDALEEVAQQLDRDVDHMVWELRPTALDDLGLRAALANYVQDWSRRV
                     GVRADLHTTGLSADRLTSEVETTLYRIAQEALTNVAKHAHADSVDVILERRGDHVSLI
                     IEDDGVGFEPGGTEAPLAGFGLLGMQERAALVGATVQVESASGEGTTVIVRMPIALTN
                     RTTA"
     gene            6927..7589
                     /locus_tag="AC16CY_21345"
     CDS             6927..7589
                     /locus_tag="AC16CY_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3356411.1"
                     /translation="MSQQTSSIRILLADDHVTVRHGLKMLIDSQADMRVVSEASDGAA
                     AVEQARTLHPDVVVMDISMPGMNGLVATRTLKQLQPDSVIVILTRHGDDAYLQELLRA
                     GADGYVLKQSAPSELLKAIRTTAGKRPYLDSALAARVTEGYLARTRKDHATGASLTDR
                     ETAVLRQIAAGHSNKEIAAQLELSVKTVEVHKANAARKLGLKGRIDIVKYAVLQGWLG
                     DA"
     gene            7711..7911
                     /locus_tag="AC16CY_21350"
     CDS             7711..7911
                     /locus_tag="AC16CY_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356412.1"
                     /translation="MTFEKRPQQPFDKAALSLVNGGGAAPEARGGNDPVTEATSFTLR
                     VKPDRRQTHTAFPPDLERRRDH"
     gene            complement(7979..>8442)
                     /locus_tag="AC16CY_21355"
     CDS             complement(7979..>8442)
                     /locus_tag="AC16CY_21355"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3356413.1"
                     /translation="IDVARFKGVDQLQGGYEITPADLKGALQRERVEIHPGDVVLLHT
                     GWGSLWMKDNARFTASAPGIGVAAGQMLADAEVVVVGADTWGVEVMPNPDSSLSAPVH
                     QLLLARNGIYIHENLITADLARDSVYEFMYVFAPLRLKGATGSPGNPLAIR"
BASE COUNT         1361 a         2830 c         2934 g         1317 t
ORIGIN      
        1 tccatgtcga tgtcgttgat gcgccctccg cccacctcga gcgcgagctt cgccgccgca
       61 agcgcttcct cgtgcgtccc cggattctct cccggggccc agatgccgac gcctgcggcc
      121 accatcagcg ccgtggtacc tcgattcgtc gtgagcgacg gatcggcccc cagctcgagg
      181 agcgtcttca tgagcggcac gtcatcattc ttggccgcca tgagaaacgg cgtcgcgcca
      241 atgcggttga gcatgttgcg attgccgtcg cgcggttcct tcgtcacccg cgcgttgacg
      301 ttggcgccgc gcttgacgag ttgacgcgcc agatcgaggc tgtcgaggct gcccgtggcc
      361 accggtcccg gcaggttgaa gccggcgttg tggcgccgcg accacgcgat ctgatggagg
      421 gctgaccatc cctgcgcctc ggcgttcgga ttcgctcccc gttccaggag gaacccggcc
      481 agctcgtagt gcgcgttcac gacggcgagc acgagcgcgc tcgtcccgtc cgacagcgac
      541 tggttcacgt cgacgcccag gtcgagcagc acgcgcgtgg agccgatgtg tccgccgcgc
      601 acggcaaaca gcagcggcgt gaaggatccc gccttcgact tgccgttgcg gtcggccccc
      661 gcttcgacga gcacccggac ggcgtcggcg ttgttgtccg ccgccgccca catcagcgcg
      721 tcctgtccct tgcggcgctc gcgcgcgcga acgtccgcac cgcgcgcgat cagcgccttg
      781 agcgtcgcga cgtcgccggt ccgggcggcc gtcatcaaca cggtctcgcc gtcggcaagc
      841 gcggcgttcg ggtccgcgcc ggcctcgagc agacgcgaga ccatggccgc atcgccgttc
      901 atcgccgcga ggtagagagg cgtgatgccg tagcggttgg ccgcggcgac cctggccccg
      961 gcgcggagca gcaggtccgc cgtcggcagg cttccggcgc gcacggccca gtgcagcgcg
     1021 gtcgtgccgt cgggctcggc gacgttgctg tcggccttgg cttgcagaag cgcgcgcacc
     1081 gcggaggtat cgccgcggcg ggcggcctcg acgagaggcg tgtccgcccc gaggcgcgcg
     1141 gcgccgacga tcgagaccag gaggaccgcg acgactctcg acttcatacg cccgacagcg
     1201 gctccacctg aagccggccc gtgctgtcac cgagcgtctc cgcgtggacg cccaccttgt
     1261 cgagcatgct gagcagaagg ttcgtcatcg gcgtctcgtt ccggaagacg agatgacgtc
     1321 cgccttgaat ctgccccgcg ccccctccga cgatggcgag cggcagatcg tagtgcgcat
     1381 gcaggtttgg attgctgagg ctcgccccgt acaggaacat cgagtggtcg agcagcgtgc
     1441 catccccttc aggcgtcgcc tgcagcttgt cgaggaacca ggcgaagagc tccgtctggt
     1501 acgtgttcaa ccgcgcgtac ttcgcgagct gattcgggtt gtcctgatgg tgcgacagcc
     1561 catggtgacc ttcggtgatg ccgatctccg gataggtgcg gaagttcagc tcgcggccga
     1621 gcatgaacgt cacgacgcgg gtcgcgtcgg cgcggaaggc cagccactga agctcgtaca
     1681 tcaacttgac gtgctggtcg aagcggtcgg ggacgccggc cggccgctcc agcgttggaa
     1741 gctcggcgcc gtcgcgcgct tcgatggcct ggatccggcg ctccacctcg cgcaccgagt
     1801 cgaggtagtc cgcgacgacc gcgcggtcgc ctgcgccgag cgacgcctgc aggcgcgcga
     1861 gatcggcgac gaccgaatcg agaatgctct ggttccgcct ggcctgggcg aggcgctgct
     1921 cgttggtgcc cccatccccg aacagccgct cgaagacgat ccgcgggttg ttctccgtgg
     1981 gcagcggcgt cgtcgccgag ctccacgaca gcgtgttgag gtacgcgcag ctgtagctgt
     2041 tctcgcactg gccgccgagg aagttgagat cgatggcgag ctcgagcgac ggcagcgcgg
     2101 tgtccttccc gagatggggg gccgcgatct ggtcggccga gatgccgttg cgcacgtctg
     2161 cgccctcagt gcgcttgcag tgcacgccgg tcagccacga cgccacgctg cgcgtatggt
     2221 ccccgtttgc gccgtcgtcg aacgcatccg cctggtgctg cgcgagcccg ctgatgacga
     2281 gcaggcgatt cttgtacggc gcgagcgggc tgaggatggg cgacagctcg aacccggttc
     2341 cctccccggt gggtgtccag tagttgatgc cggtgaagtt ccgtgcgaca ccgttcggca
     2401 ggtagatgaa ccccaggcgc cggacgggtt ttgccgccgt ctgcgacgtc gccgagagcg
     2461 ccgggaccat cgcgtcgagc agcggcagcc cgagcgccgc tcccatgccg cgcaggaacg
     2521 ttcgacgagg aagagccttt ctggtaatga acatctaccc tccagccgcc ttccgcatcc
     2581 ggaacggcgc gctctgcacc acgccaagga cgagcgtcga gaagcggtac tcgtcgcggg
     2641 acccgtcgcg aaggatcgcg cgcaccgcgg gcgcgtcgta gtactcgagc ccgcggccga
     2701 gcgcgtaggt catcatcttc tcggtcagcg tggccacgaa ccggtccgat cggagcagcg
     2761 cggctcgtaa gccgtcggcg ccctcgaact tcattccatc gggcgctaca ccgctgacat
     2821 cgatcggcgc ggccgactcg cccagcgtgc gccagcgtcc aatcgcgtcg aagttctcga
     2881 gcgacagccc cagcggatcc atcatcgcgt ggcaggacgc gcagacgggg ttggcgcgat
     2941 gccgctccat gcgctggcgc atggaaagca cctgtccctc ggctgtggtc gccctcagct
     3001 gcggcacgtt gggcagcggc ggcggcggcg gcgagcccag gaggttctcg agaatccatt
     3061 ttccccgaac gacgggcgag gtccggtcgg gataagaggt gaccgtcagg atgctgccct
     3121 gaccgagcaa cccgcggcgc ttcgtgtccg acccgagcgt cacgcgccgg aaatggttgc
     3181 ccttgacgtt cggaatgccg tagtgctccg ccagccgttc gttcacgaag gtgtagtcgg
     3241 cgcgcagcag gtcgagggcg ctgcgatctt cgcggacgat gctgtcgaaa aagagctcgg
     3301 tctcgcgcct gaacccctgg cgcaaggcgt cgtcgaagtc cggaaagatg ctctgcaccg
     3361 ggacgacggc gtcgagattc cgcaggaaca gccactggcc cgcgaagttc ttcacgaacg
     3421 cgtcggcgcg agggtcggcg atcatccgcc gcacttgcgc ggtcaacacg gcggcatcgc
     3481 cgagcctgtt gttcaccgca agatcgagca actgatcgtc ggggatgctg ctccagagga
     3541 agaaggacag tctcgacgcg agctcgatat cgctgatgcg gtagacgccc gcgactccgg
     3601 cgggccgcct ctgcgcgtct ggcgcgctcc ggcgagcctc gccggagcct tcggcggagg
     3661 cgggatcccg ctcgacgcgc aggaggaact ccgggctcgc gagcaggcgg cggagcgcgt
     3721 gctcgacgcc catgtcgaaa ccgccctgcg cgcgtccctc gtcgaagaac gccatgagcg
     3781 tgtcgacgtc ggccgacgcg gcgggccggc gataggcgcg gcgcgccaga gtcgagagga
     3841 tctgccgcgc gcacgtcctt tccgcggcgg catcgggagc ggaggccggc cgcccggacg
     3901 atgaagacga tggatgacac acgaagatgc gcttgcggct gggcgcattc cccgaaccgg
     3961 tggagctata aggaccgctg atctcgacgc tccgcaggta cgcgccgaga cgcgtctccg
     4021 gaatgttcac acccgcgggg tacggacgct cgaacggaag cctcgccgtc tcgtcgagcg
     4081 cggacacgcg gttgaggaag gtcaccacga cgtcgcgggc gccggccttg acgggcacgc
     4141 gcaggttcca cgtctcgtcg gcgcgattgc gggacgcgcg ttccttcgcg ctcgcgcgca
     4201 cggtctgttg aatctgcgag atcgccggcc gggcgggctg cccctggccg cccgggcgct
     4261 gcggtggagc gctcgccggc tgcgccggcg gctcggacgg gttgtcgggg tcctccggcg
     4321 gctgcgcgtt tccggctccc gagacgccag gcagtgtgaa gaggccgacg cgttcaccgt
     4381 cgatgctgat ctcgagctgc tgctcctctg cgtagagcgg cacgctctcg ttcaggtcgc
     4441 gcgtcaggcg cggacgaaac tcgtacacgc cgtccatcgg gaaggtgtag cgaacgagcg
     4501 tgccgccgcg cgagccgagc ggcagcccgg gcaggtgctc gtcctgcgac aggtcgtcag
     4561 cgacgcgata gtagtcgata ctcggcacgg gcagcggcgt cccgatcgcg agccggctga
     4621 ctttctgggc ggccgtcaga tagcgctcca tcagcgtcgg cgacatcttg agcacgcccg
     4681 cgatgttgtc gaacccgtag ctcacgtcgt cggcgggcag cagcgcggcg acgtcaacgt
     4741 cgagatcgag cagatcccgg acggcgtttc gatactcggc ccggttgagg cgatggatcg
     4801 gctcggtgcg gcccgggttg acgtttgcac gcgacgcgcg atcgagctcg gcctcgagcc
     4861 acgaggcgag cgcgtcgtgc gtcgccttgt ccggccgagg cgagcttgcc ggaggcatca
     4921 ggcctgcccg cagcttcagg acgaccttct cccagacctg ggcgtcgtcg ccgacgcgcg
     4981 ccaggtcgag cccttcgagc gagatcggaa ccgtgccctg ggtcttcagc cgctgggtgt
     5041 ggcaggtcag acagtaccga tcgagcgtgg cgcggtgagc cgcttcgggg gctgcgggcg
     5101 ccgcctgctt gggggccgcc tgcgtgtgac ggccggacca gacggccgcc aggccgagcg
     5161 ccaacacggt ggaaaacagg agcggagcgc ggcgagcgat tcgcctccgg cgggtgccgc
     5221 ggtgtcccat aactctggct ccaatcctag cgtcttttga cgcggggatc agccccccgc
     5281 ccacctgaca ggggcgcggc gccagagtag actcttgcgt ctgactgatg ccgcagccgg
     5341 ccaaggtttc gtaggggccg gtcgagtgaa cctcggtcgc cgggagacag cgtgcacgaa
     5401 tcaggagtgc actatcgcca actcgtggat ttgtcgccag acggcatcct cgtcagccgc
     5461 gacaaccgga ttgtgttcgt caatccggcc gcgatgcgcc tgctcggcgc ggtctcgccc
     5521 gatcggattc tcggacagcc gccgctgcga ttctttccgc ccgatcgaca cgcgcacctt
     5581 cgcgaacggt ttcggcagct ggcgatgggc aaggccgttc ctccggccga ggagacgctc
     5641 gtcggcatca cgggcgtgac gacggacgtg gagttggcga gctcgctctt cgaggatccc
     5701 ccggaccggg cgattcaact gatcgtgcgg gacatcagcg agcggaagcg cacggagtcc
     5761 gcgctccgcg agagcgagga acgactgtcg ctggcgtttg ccggcgcgca ggagggcgtc
     5821 tgggactgga acctcgaaac gggcgcggtg gtctactcgt cgcactggaa acagatgctc
     5881 ggctacaccg aggacgagat cgagccgcac gtgagcgcgt gggagcgcct gctgcacgcg
     5941 gaagacaagc cgcggacgca ggaagtgttc gacggggtgg ttcgcggcga gcggaactac
     6001 acgatggagt tccggcttcg gcacaaggcc ggccattacg tgcacgtgct ctcgcgcggt
     6061 tttcccgtgc gacgggagga cgggagggtc gttcgcatcg tcgggacgca cttcgacctg
     6121 accgaacgga aacaggctga acaggccctg aggcgcgcgc aggaggagct cgagctgcgc
     6181 gtccgtgagc gaaccggcga gctggcgctc gccaacgtgt cgctgcgcgc ggagatggcg
     6241 gagcgggagc gcgcggaacg ggcgcgcacg gagctgctct cgcggctcgt gttcgcccag
     6301 gaggacgagc ggcggcggat cgcacgcgag atgcacgatc agttcgggga acaactgacg
     6361 gcgctcgggc tcggcatccg ggcgctcaag cgcgcgttcg aggaccaccc ggccctgcgc
     6421 gcacaggtgg acgcgctcga agaggtcgca cagcagctcg atcgcgacgt cgatcacatg
     6481 gtctgggagc tgcggccgac cgcgctcgac gacctcggcc tccgcgcggc gctcgccaac
     6541 tacgtgcagg actggtcgcg ccgcgtgggt gtgcgcgccg atctccacac gacggggctc
     6601 agcgccgatc gactgacgtc ggaagtcgaa acgacgctct accgcatcgc ccaggaagcg
     6661 ttgaccaacg tcgccaagca cgcgcacgcc gacagcgtgg acgtcatcct ggagcgccgc
     6721 ggcgaccatg tctccctgat catcgaagac gacggcgtgg ggttcgagcc gggcggcacc
     6781 gaggcgcccc tcgcgggatt cgggctgctc ggcatgcagg aaagggcggc actggtcggc
     6841 gcgaccgtcc aggtcgagtc ggcgtcgggt gaagggacca cggtcatcgt gcgcatgccc
     6901 atcgccctca cgaaccgcac gacagcatga gtcagcagac gtcgagcatc cgcattctcc
     6961 tggccgacga tcacgtgacc gtccggcatg gcctgaagat gctcatcgac agccaggccg
     7021 acatgcgcgt cgtctcggag gcgagcgacg gcgccgcggc cgtcgagcag gcgcgcacgc
     7081 tccatcccga cgtcgtcgtc atggacatct cgatgccggg catgaacggg ctggtcgcca
     7141 cgcggacgct caagcagctg cagccggact cggtgatcgt gatcctcacg cggcacggcg
     7201 acgacgcgta cttgcaggag ctgctgcgcg cgggcgccga cggctatgtg ttgaagcaga
     7261 gcgccccctc ggaactgctg aaggcaatca ggaccacggc gggaaaacgg ccttacctcg
     7321 attccgccct cgccgcgcgg gtcaccgagg gatacctcgc gagaacgcgc aaggaccacg
     7381 cgaccggagc cagcctcacg gatcgagaaa cggctgtgct gcgacagatc gccgcaggcc
     7441 acagcaacaa ggagatcgcg gctcaactcg agctcagcgt gaaaacggtc gaggtgcaca
     7501 aggccaacgc cgcgagaaag cttggcctca aaggccgcat cgacatcgtc aagtacgcgg
     7561 tccttcaggg ctggctgggc gacgcgtgat ttttctcatg cacaatcatt agggattctc
     7621 cctaatctca cccggcatga tcccaatggt cgcgcggctc gcgagagtgt aaagtcccgc
     7681 gcatccccag tgcctgcgat cagtccgacg atgacgttcg agaagagacc ccagcagcct
     7741 ttcgacaagg cggcgctgag tctcgtcaac ggaggcggcg cggctcccga ggcgcgcggc
     7801 ggtaacgatc ctgtaacaga ggcgacgtcc ttcacgcttc gcgtcaagcc ggatcgacgg
     7861 cagacgcaca cggcgttccc acccgacctc gagcggcgac gcgaccacta gccgaaaaag
     7921 ggggtcggga gtcattttcc cggagcaaca cggaaatgac tcccgacccc ctttattgct
     7981 agcgtatcgc cagcggattg cccggcgacc ccgtcgcccc cttcagcctg agcggcgcaa
     8041 agacgtacat gaactcgtag acactgtcgc gcgcgaggtc ggccgtgatc aggttctcgt
     8101 ggatataaat gccgttgcgc gccagcagga gctggtgcac cggcgccgag agtgagctgt
     8161 ccggattggg catcacctcg acgccccacg tgtcggctcc gacgacgacg acctcggcgt
     8221 cggcaagcat ctgccccgcg gcaacgccga tgccgggcgc gctggcggtg aagcgcgcgt
     8281 tgtctttcat ccagagcgat ccccacccgg tgtgcaggag cacgacgtcg cccgggtgaa
     8341 tctcgacgcg ctcgcgctgg agcgcgccct tgagatcggc cggcgtgatc tcgtatccgc
     8401 cctgcagctg atcgacgccc ttgaaccgcg ccacatcgat ga
//
LOCUS       DBNGSH010000932         5287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1310273, whole genome shotgun sequence.
ACCESSION   DBNGSH010000932 DBNGSH010000000
VERSION     DBNGSH010000932.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1310273"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC16CY_21360"
     CDS             <1..252
                     /locus_tag="AC16CY_21360"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3356414.1"
                     /translation="VAPGKVSSVQPPWPSGLVSLNAAPWAQVWIDGQSVGETPLSNIR
                     VPIGSHEVVFRHPELGEKLVRAVVTAGTPTKVSADLRQK"
     gene            258..1181
                     /locus_tag="AC16CY_21365"
     CDS             258..1181
                     /locus_tag="AC16CY_21365"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3356415.1"
                     /translation="MRTHVIALTCVVLVVVAAPRVSAQESLDAARELYASAEYDSALA
                     MLDGLLRNGSGGEDRHSIEMYRVLCLMAMRRDGEARGAIETLVVRNPMYRPAGDLPPR
                     IRNVYDETRRRVLPSAVQAKYQEAKTAYDAKDYVSARRGFSEVLEVLADPDMAAAANQ
                     SPLADVRTLARGFEELSAKAIVPSGRVAASTEQPMVPATPVPQPPPPTPRVEKVYTAA
                     DTNVVPPVTINQRIPAFPERVRVPRTGIVEVVIDPTGAVESATMIEPLTPQIDRLTLA
                     ATRTWQYQPATVNGVPVKYVKRIQVSVVPSP"
     gene            1373..3670
                     /locus_tag="AC16CY_21370"
     CDS             1373..3670
                     /locus_tag="AC16CY_21370"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HIR3356416.1"
                     /translation="MNVLILRTGRPSLAASLLLWLLAVSPASADTLTVTWDPNSQTVA
                     GYVVYMGTQSGSLTQRYDVGNTITFTNPNAVPGQVYCFQVAAYFVPTAEGPRSAQVCG
                     YADQYPTLANPGNQSSAIGQQVGLQLLGNDPDGLPVTYNATGLPAGLTLGTGTGFIGG
                     APTSAGTYTVTARVSDGVLQSAPQTFTWSVTTAPAVDTTPPSVSITVPTSNSSYTALS
                     STLGVSGTATDNVGVTAVTWTNDRGGSGTATGTTSWSVPSIALQSGTNIITIRARDAA
                     GNQGMDVLTVTYSTSSEGDPIAPTVSISGPTSNSSYAALSSTLAVSGTASDNIGVTAV
                     TWTNDRGGSGTAAGTTSWSVPSIALQSGANVITIRARDAKGNQGVDVLTVTYSLADSI
                     PPTVTISTPTTQSTLATTSSSLTIGGTASDNVGVTQVTWVNDRGGSGTANGTSSWSVG
                     GIILQTGTNVITVRARDAAGNQGTDVLTMTYSTTSEDVIAPTISIAGPTTGPSYSTTT
                     SVVTLGGSSSDNVGVTAVTWTNNRGGAGFSSGTTSWSIPSVPLQGGTNVITVTAQDAA
                     NNKGTDVLTVTYNAPTVPAPDTTPPSVSISMPTSQPSYATTESGMALGGTSSDNVGVT
                     QVSWVNNRGGSGVASGTTSWSVPAVALQTGDNVITVTARDAAGNQSSDVLTVSFSAPA
                     PTPSPSIVLSATATYVGGGDRLRVILRWTQAQGRTVTVYRNTKVVTTTSNDNYTDRPG
                     GQGPFSYRVCLTGTTVCSNTVTVTR"
     gene            complement(3691..5115)
                     /locus_tag="AC16CY_21375"
     CDS             complement(3691..5115)
                     /locus_tag="AC16CY_21375"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3356417.1"
                     /translation="MHKTVQTTDLRLSELLAGLSHALDLTEGQREGHAVRTCLIGMRL
                     AQVLRLPAAQRSSLFYALLMKDLGCSSNAARFAALFGADDHRVKADLKVTDWSQALEA
                     FRFVSRNVAPGHFWLRRVWQLLGVLSRGPEGAREVVVTRCERGADIARMLGFPGETAE
                     AIRALDEHWDGAGQPYSLRREAIPLLARILGLAQTVEVFFTAHGLDAAYDMAVSRRST
                     WFDPVVVEALLAMRKDLNFWHSLADGDPLPLVSAMEPQEHIQTVDEDRLDLVAEAFAL
                     VIDAKSPWTFRHSKGVADLSVAIARQMGYREHQLRELRRAALLHDIGKLGVSSLILDK
                     PGTLTDGEFAIMRQHPAATRAILRRTGCFSGLADVASSHHERLDGRGYDRGVANLPMV
                     ARVLSVADICDALGASRPYRDGMPPDRVLDIMGREVGTAIDAECFAALQAVLPTLTPA
                     RTQEQGTPAAICVPSLAEDYRQAA"
BASE COUNT          928 a         1794 c         1741 g          824 t
ORIGIN      
        1 gtcgcgcccg gaaaagtgtc gtccgtgcag ccgccctggc cgagcgggct ggtgtccctc
       61 aacgcggccc cctgggccca ggtctggatc gacggccagt ctgtcggcga gaccccgctc
      121 agcaacatca gggtgccgat tggatcgcac gaggtagtgt tccgccaccc tgagctcggc
      181 gaaaagctgg tccgcgcggt cgtgaccgcg gggacgccga ccaaggtgag cgccgatctg
      241 aggcagaagt aatggccatg cgtactcatg tcatcgctct cacgtgcgtc gtcctcgtcg
      301 ttgtcgccgc gccccgtgtg agcgcccagg aatcgctcga cgccgcgcgc gagctgtatg
      361 cctccgcgga atacgattcg gccctggcca tgctcgacgg cctgttgaga aacggcagcg
      421 ggggagagga ccggcactcc atcgagatgt atcgcgtgct gtgcctgatg gcgatgcggc
      481 gcgacggcga ggcgcggggt gcgatcgaga cgctggtcgt tcgcaacccg atgtaccgcc
      541 ccgccggcga cctgcctccg cggatccgca acgtgtacga cgagacgcgc cggcgcgtcc
      601 tgccatcagc cgtccaggcg aagtaccagg aggcgaagac ggcgtacgac gccaaggact
      661 acgtcagcgc gcgccgcggc ttcagcgagg tgctcgaggt gctggccgat ccggacatgg
      721 ccgcagcggc caatcagtcg cccctggcgg acgttcgcac gctggcgagg ggtttcgagg
      781 aactgagcgc gaaggcgatc gtcccctccg ggcgcgtggc cgcgtccact gagcagccga
      841 tggtcccggc cacccccgtg ccgcagccgc ctccgcccac gccccgcgtg gagaaggtct
      901 acaccgccgc tgacacgaac gtcgttccgc ccgtcacgat caatcagcgg attccggcgt
      961 ttcccgagcg ggtccgcgtc ccgcgcaccg gcatcgtgga ggtcgtcatc gacccgaccg
     1021 gcgccgtcga gtccgccacg atgatcgagc cgctcacgcc gcaaatcgac cggctcacgc
     1081 tcgcagcaac gcggacctgg cagtatcagc ccgcaaccgt gaacggggtc cccgtgaaat
     1141 acgtgaagcg aattcaggtc agcgtcgtcc ccagcccgta gatccgtccg ctcacacaat
     1201 ctgtaagccc gccgacagaa ctggaactta ttgcatcatg gtcgcgcgga tcagcttggg
     1261 ctaagttaca cgacgcaggc cgtttggctt gcgtaccgtc acgcgggcgc gatcttgcta
     1321 cgtaggccct cgggactcgc gacctccccc cgggcgctcc ctcacgcacc agatgaacgt
     1381 gctgatactg aggactggtc gcccctcgct tgccgcctcg ctgctcctgt ggctgctcgc
     1441 cgtgagtccg gcgtctgccg acacgctcac ggtgacgtgg gacccgaata gtcagacagt
     1501 tgccggctac gtcgtctaca tgggcacgca gtcaggcagc ctcacgcagc gctacgacgt
     1561 cggcaacacc ataaccttca cgaacccgaa cgccgtgccg gggcaggtgt actgcttcca
     1621 ggtggccgcg tatttcgttc caaccgcgga ggggccgcgt tcggcccagg tgtgcggcta
     1681 cgccgaccag tacccaactc tggccaatcc ggggaaccaa tcgtcagcga ttgggcaaca
     1741 ggtcgggctc cagttgctcg gcaacgaccc ggatgggctt cccgtgacct ataacgcgac
     1801 ggggctgccg gccgggttga cgctcggaac gggtaccggg ttcatcggcg gcgcgccaac
     1861 ctcggccgga acctacacgg tgacggcgcg cgtatctgac ggcgtccttc agtcggcccc
     1921 gcagacgttc acctggtccg tgacgacggc accggccgtg gacacgacgc cgccgtcggt
     1981 gtcgatcacc gttccgacct cgaactcgag ctacaccgcg ctgtcctcca cgctcggcgt
     2041 cagtggtact gccaccgaca acgtgggcgt cacggcagtg acgtggacca atgatcgcgg
     2101 gggcagcggg accgcaaccg ggaccacgag ctggagcgtg ccgtcaatcg ctctgcagtc
     2161 aggtacgaac atcatcacga tcagagcgcg cgatgccgct ggtaatcagg gcatggatgt
     2221 cttgacggtg acctactcca catcatctga gggggatccg atcgcgccaa cggtctccat
     2281 tagcggtccg acgtcgaact cgagctacgc cgcgttgtct tccacgctcg cggtcagcgg
     2341 cactgccagc gacaacatcg gtgtcacggc cgtgacgtgg accaatgacc ggggtggcag
     2401 cgggaccgca gcggggacga cgagctggag cgtgccgtcg atcgctctgc agtcgggcgc
     2461 gaacgtcatc acgatcagag cgcgcgacgc caagggcaac cagggggtag acgtgttgac
     2521 ggtgacctac tccctcgccg actcgatccc gccgaccgtg acgatctcca cgccgacgac
     2581 tcagtccacg ttggccacca catcgtccag cttgacgatc ggaggcacgg cctcggacaa
     2641 cgtcggagtc acacaggtca cctgggtcaa cgatcgtggc ggctccggaa cggcgaacgg
     2701 cacgtccagc tggagcgtcg gcggaatcat tctgcagacg ggcaccaacg tcatcactgt
     2761 gagagcgcgc gacgccgcgg gcaatcaggg cacggatgtc ttgacgatga cctactccac
     2821 cacatctgag gacgtgatcg caccgacgat cagcattgct ggcccgacga cgggccccag
     2881 ttattccacg acgacctccg tggtaacgct gggtggctcg tcatccgaca acgtcggggt
     2941 cacagccgtg acgtggacca acaatcgcgg cggcgccggt ttctcgagcg gcacgacgag
     3001 ctggagcatc ccgtccgtgc ctctgcaggg cggcaccaac gtgatcacgg tgacggcaca
     3061 ggacgcggcc aacaacaagg gcaccgacgt gctcacggtc acctacaacg ctcccacggt
     3121 gcccgcccca gacacgacgc cgccctcggt gagcatctcg atgccgacgt cgcaaccgag
     3181 ttacgccacg acggagtccg gcatggctct cggcggaacc tcgtcagaca acgtgggtgt
     3241 gactcaggtg agctgggtca acaatcgcgg cggcagcggc gtcgccagcg gcacgacgag
     3301 ctggagcgtg cctgccgtgg cgttgcagac gggggacaac gtgattacgg tgacggcaag
     3361 ggacgcggcg ggcaaccagt cgagtgacgt gttgacggtg agcttttcgg cgccggctcc
     3421 gactccctcg ccgtcgatag ttctgtcggc aactgccacc tacgtcggcg gcggggatag
     3481 actcagggtg atcctccggt ggacgcaagc gcagggtcgt accgtcaccg tgtatcgaaa
     3541 caccaaggtc gtgaccacaa cgagcaacga caattacacc gacaggccag gaggccaggg
     3601 cccgttcagc taccgcgtct gcctgacggg aacgaccgtg tgttcgaaca cggtcaccgt
     3661 gaccagatga cacgagccgt caggccgcgc tcacgcggcc tgccggtagt cttccgccag
     3721 gctgggcacg cagatcgcgg caggcgtacc ctgctcctgc gtgcgggcag gagtcagcgt
     3781 cgggagaacc gcttggagcg cggcaaagca ctccgcgtcg atggccgttc cgacctcccg
     3841 tcccatgatg tcgagcacgc gatcgggcgg catcccgtcg cggtacggcc gcgacgcccc
     3901 gagcgcgtcg cagatgtctg cgaccgacag cacgcgagcg accatcggca gattggcgac
     3961 gccgcgatcg tagccgcgcc cgtcgagccg ctcgtggtgc gacgaagcca cgtcggcgag
     4021 tccgctgaaa cagcctgtgc ggcggaggat cgctcgcgtc gcggccggat gctgccgcat
     4081 gatggcgaac tccccgtcgg tcaacgtgcc gggtttgtcg agaatgaggc tcgagacacc
     4141 cagcttgccg atgtcgtgca gcaaggccgc gcgacgaagc tcgcgcagct gatgctcccg
     4201 gtagcccatc tggcgcgcga tggcgacgct cagatcggcc acgcccttgg agtgccggaa
     4261 cgtccacggg gacttggcgt cgatgacgag cgcgaacgcc tcggccacca gatcgagccg
     4321 atcctcgtcg accgtctgga tgtgttcctg cggctccatt gccgacacga gcggaagcgg
     4381 gtcgccgtcg gccagcgagt gccagaaatt gaggtccttc cgcatcgcga gcagcgcctc
     4441 gacgacgacg ggatcgaacc aggtgcttcg gcgggagacg gccatgtcgt aggcggcatc
     4501 gagcccgtgg gcggtgaaga acacttcgac ggtttgcgca agcccgagga tgcgtgcgag
     4561 cagcgggatt gcctcgcgcc tgagcgagta cggttgcccc gctccgtccc aatgctcgtc
     4621 gagcgcgcga atggcctccg cggtctcgcc ggggaacccc agcatgcggg cgatgtcggc
     4681 gccgcgctcg cagcgcgtga cgacgacctc tcgcgcgccc tccggtccgc gcgacaggac
     4741 gcccagcagc tgccacacgc gtcgaagcca gaagtgtccg ggtgccacgt ttctcgagac
     4801 gaaccggaag gcttcgagcg cctgcgacca gtcagtcacc ttcaggtcgg ccttcacgcg
     4861 gtgatcgtcg gcgccgaaca gcgccgcgaa tcgcgcggcg ttgctcgagc agcccaggtc
     4921 cttcatcagg agcgcgtaga acagcgacga tcgctgcgcc gcgggcagac gcaacacctg
     4981 cgcaaggcgc atcccgatga gacaggtccg caccgcgtgt ccctcgcgct gcccttcggt
     5041 tagatccagc gcgtgggaga gccccgcaag cagctcggag aggcgcaggt cggttgtctg
     5101 cacggtcttg tgcacgactg catcgatggc aacgagcctg ccgggcgcct gtctgcgcgc
     5161 ggctgcacag ccggctcagc gcatctcttt gactgtgaag atcttcgtcg ccgtcgacgg
     5221 atccgtcatt ccttgacgcc ggctggaata aaggcggtac ttgcccagac ttccagccgg
     5281 tgccgcc
//
LOCUS       DBNGSH010000933         3590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2669325, whole genome shotgun sequence.
ACCESSION   DBNGSH010000933 DBNGSH010000000
VERSION     DBNGSH010000933.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2669325"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..711)
                     /locus_tag="AC16CY_21380"
     CDS             complement(316..711)
                     /locus_tag="AC16CY_21380"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR3356418.1"
                     /translation="MKRFLRFNGVGALGIGVQLSTVAALTAWLHVNVAAATAIGVAAA
                     VVHNFIWHREWTWADRRAEGRHVLLTFCSFVLSNGAVSLVGNVALASILVWWPGLDGV
                     TANIVAIGACGVVNYWLGDRVVFPNLTIP"
     gene            complement(708..1394)
                     /locus_tag="AC16CY_21385"
     CDS             complement(708..1394)
                     /locus_tag="AC16CY_21385"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0017169 - CDP-alcohol
                     phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3356419.1"
                     /translation="MNAYTAATRLHTSVLSATERRVLIWLAGRMPGWVNSDHLTGLAL
                     AAMVGAGLSYWLASVTPIGLVLVIVFLAINWFGDSLDGTLARVRNCQRPRYGFYVDHV
                     VDEIGIAFLLGGLALSGYMSPAVAIGVLVAYLMLLVEVFLATHALGTFRMSYFKLGPT
                     ELRILLAIGNVIVISRPTTMFFGREVLFFDVGGVVAAVALVLTLIVSAVSNARTLYRA
                     EPLPMPRIAE"
     gene            1506..2357
                     /locus_tag="AC16CY_21390"
     CDS             1506..2357
                     /locus_tag="AC16CY_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356420.1"
                     /translation="MSPARVRRRPAPALALAVSLLVETSLVAGSLKAETAAGWAAYLA
                     ATERRRAGELADSRRFLVMDHQPTPAEDRRAVLSGAVVVAPMSTVERGGLVAMPWGLV
                     HHWRGAVLVKGTTVSELMSRIQHATPPALQEDVLRSSVIARGPDAMKVYLRLQRSKVI
                     RVVYDTEHDVRFEHIDDRRAASTSTATKIAELKYPGTAAEYELPLGSDRGFLWGLNAY
                     WRYEATPGGTIAECESISLSQDVPAALRFIAGPLIRDAARESMERTLTALRDRFGRKP
                     PAPSPAR"
     gene            complement(2245..>3590)
                     /locus_tag="AC16CY_21395"
     CDS             complement(2245..>3590)
                     /locus_tag="AC16CY_21395"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3356421.1"
                     /translation="RLPDRVGPYRPLRVLGVGGMGVVYLAEDTRLGRTVALKALAPWL
                     VDNAAQRERLRREARAAASLNHPGIATIYALEEIDDNVYIASEYVPGETLRDEIARGP
                     LEPLRAIDTMIGIARALAAAHQRGIVHRDLKPENVMRTPDGDVKILDFGLAQSSDLPS
                     APSVPSRGVMTFGTPLYMSPEQIRGDAVDQRSDVFSLGVVLYELVTGATPFASADPKS
                     TIADDAPGHVSRISLRHVERVVLVCLRVSPEARFASAADLLRALEEARSHLTSADHDR
                     KAAPVPAGPAPREGHAIWWWQFHQAAATLVYLLLLIPLWRVRDLAPWSSGTLLFVVGL
                     VGVVIASALRWHLWFAVRLDPAAWHDQHGRSRRWILAGDALFVVILVSVGVFSLLAER
                     HWGILLLAAAAAVLVSFAVIEPATARAAFGQTYPSTPSRSAPSIREPRLESEDRR"
BASE COUNT          662 a         1213 c         1201 g          514 t
ORIGIN      
        1 gactgcccgt cccgaagcag cgtgaccgcc gcgccgggca ctcgcgcgcc gagcgcgtca
       61 acgacgacgc cggtcagcgt gccctggctc tgggcggaca ggcgtgtcgc cgcgcctgcc
      121 ccggccacca ctaccatcag cgcgcacatg agtcgcttca cgaaatcgag tcctcctgtc
      181 ggcctccggt ccggcgcgac cccggcatta gaacctgagt tcgccgcgcg gtgcctgaag
      241 acttcatgaa ttcttcatca gacgacacac gggggcacgt gtgccgtttt cgcgggaggc
      301 atcctgacga aaacttcagg gaatcgtaag gttagggaac acaacccgat cgccgagcca
      361 gtagttgacg accccgcacg cgccaatcgc cacgatgttg gccgtgaccc cgtcgagccc
      421 cggccaccag accaggattg aggccagcgc cacgttcccc acgagcgaca cggcgccgtt
      481 cgagaggacg aatgagcaga acgtcagcag cacatgccgg ccctccgcgc gtcgatcggc
      541 ccacgtccac tcgcggtgcc agatgaagtt gtgcacgaca gccgccgcca cgccgatcgc
      601 ggtcgcggca gccacgttga cgtgcaacca ggctgtcaac gcagcgaccg tcgagagctg
      661 aacgccgatg ccgagcgcgc cgacgccgtt gaacctcagg aatcgtttca ctcggcgatc
      721 cggggcatcg gcagcggctc tgcccgatag agtgttcgcg cgttcgagac cgccgacacg
      781 atcagcgtga gcacgagggc gaccgccgcg accacgcctc cgacgtcgaa aaagagcact
      841 tcgcgaccga agaacatcgt cgtcggacgg gatatgacga tgacgttgcc gatggccagc
      901 aggatgcgca gctcggttgg accgagcttg aagtagctca tgcgaaacgt gccgagcgcg
      961 tgcgtcgcca gaaacacttc gacgaggagc atcaggtacg cgaccagcac tccgatggcc
     1021 acagccgggc tcatgtagcc cgagagcgcg aggcctccca gcagaaatgc gatgccgatt
     1081 tcgtccacga cgtgatcgac gtagaacccg tagcgaggcc gctggcaatt ccgcacacgc
     1141 gcgagcgtgc cgtcgaggct gtctccgaac cagttgatgg cgagaaagac aatgacgagg
     1201 acgaggccga tcggcgtgac gctcgcaagc cagtaggaca gtcccgcgcc aaccatcgcg
     1261 gcgagcgcga ggcccgtgag atggtccgag ttcacccagc ccggcattcg cccggcaagc
     1321 cagatcagca cgcgtctttc tgtcgccgac agcacgctcg tgtgcaggcg cgtggcggct
     1381 gtataggcat tcattccata accccccgag ggggaatgcg taaaacgggc cgaacggggc
     1441 tcagggccga cgagctcggg caatcgacat gagccgtttc ggcacgattc gacgcattcg
     1501 tccgtatgag ccccgcccgc gttcgacgcc ggccggcacc ggcactcgcg ctcgcggtca
     1561 gcctgctcgt cgagaccagc ctcgtcgctg gcagcttgaa ggctgagacc gccgccggct
     1621 gggcggcgta tctcgccgcc accgaacgcc ggcgtgccgg ggaactggcc gactcccgcc
     1681 ggttcctggt gatggaccat cagccgactc cggcagagga tcggcgcgcc gtgctctccg
     1741 gcgccgtcgt cgttgctccg atgtccacgg ttgagagagg cgggctggtt gccatgccat
     1801 ggggcctcgt gcaccactgg cgcggcgccg tcctcgtcaa aggcacgacc gtttccgagc
     1861 tgatgtcgag gattcagcac gcgacaccgc cggcgcttca ggaggacgtg cttcgatcgt
     1921 cggtcatcgc ccgcggtccc gatgccatga aggtgtacct gaggctccag cggtcgaagg
     1981 ttataagggt cgtctacgac accgagcacg acgtccggtt cgaacacatt gacgaccggc
     2041 gcgcggccag cacgagcacc gcaacgaaga ttgcggagtt gaaataccca gggaccgcgg
     2101 ccgagtacga attaccgctc ggcagcgatc ggggattcct ctgggggctg aatgcgtact
     2161 ggcgctacga ggccacaccg ggcggaacga ttgccgaatg cgagtccatc agcctcagcc
     2221 aggacgtccc ggccgcgctg cggttcatcg ccggtcctct gattcgagac gcggctcgcg
     2281 aatcgatgga gcggaccttg acggcgttga gggataggtt tggccgaaag ccgcccgcgc
     2341 cgtcgccggc tcgatgacgg caaacgacac gagaacggcg gcggccgccg cgaggagcag
     2401 gattccccag tgccgctcgg ccagcaggga gaacaccccg acggacacga gaatgacgac
     2461 gaacagcgcg tcgccggcga ggatccatcg gcgggagcga ccgtgctggt cgtgccaggc
     2521 cgcaggatcg agacgcacgg caaaccacaa gtgccagcgg agcgcgctcg cgatcacgac
     2581 tcccaccagg ccaacgacga acagcagcgt cccgctcgac cacggggcca gatcgcgcac
     2641 gcgccagagt gggatcagga gcagcagata gaccagcgtt gctgcggcct ggtggaactg
     2701 ccaccaccag atcgcgtgac cctcgcgcgg cgcgggaccg gcgggtaccg gcgcggcctt
     2761 ccggtcgtga tcggcggacg tgagatggct gcgtgcctct tcgagcgccc gcaggagatc
     2821 ggcggccgac gcgaaccgcg cctccggtga cacgcgcagg cacaccagca ccacccgttc
     2881 gacgtggcgc agcgatatcc gcgagacgtg cccgggggca tcgtccgcga tcgtcgattt
     2941 gggatcggcg ctcgcgaacg gagtggcgcc cgtcaccagc tcgtagagca cgacgccgag
     3001 cgagaagacg tccgaccgct gatcgaccgc gtcgccgcga atctgttccg gggacatgta
     3061 gagcggcgtg ccgaacgtca tgacgccgcg gcttggcaca gagggagcgg acggcagatc
     3121 gctcgattgc gccaggccga agtcgagaat cttcacgtcg ccgtccggcg tgcgcatgac
     3181 gttttcgggc ttcagatcgc gatgcacgat gcctcgctga tgggcggcgg ccagcgcgcg
     3241 cgcgatgccg atcatcgtgt cgatcgcccg cagcggctcg agcgggccgc gagcgatctc
     3301 gtcgcgaagc gtctcgcccg gcacgtactc ggacgcgatg tacacgttgt cgtcgatctc
     3361 ctcgagcgcg tagatcgtcg cgataccggg gtggttaagc gaggccgcgg ccctcgcctc
     3421 gcggcgcagg cgctcccgct gcgcagcgtt gtcgaccagc catggcgcca gcgccttcag
     3481 cgccacggtt cggccgagcc gcgtgtcttc ggccaggtac acgacgccca ttccacccac
     3541 gccgagtacg cggagcgggc ggtacggacc cacgcgatcc ggaaggcggg
//
LOCUS       DBNGSH010000934         4631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_462270, whole genome shotgun sequence.
ACCESSION   DBNGSH010000934 DBNGSH010000000
VERSION     DBNGSH010000934.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_462270"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..527)
                     /locus_tag="AC16CY_21400"
     CDS             complement(159..527)
                     /locus_tag="AC16CY_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3356422.1"
                     /translation="MIKGLATTIYKVTDLERAKTWYTAAFQQAPYFDEPFYVGFNIAG
                     YELGLDPDQKSGKAGPGGSVAYWRVAGIDEAVRHFVSAGASVVVPAHDVGESIKVATV
                     ADPFGNLIGLIENPHFSLPA"
     gene            complement(551..2116)
                     /locus_tag="AC16CY_21405"
     CDS             complement(551..2116)
                     /locus_tag="AC16CY_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3356423.1"
                     /translation="MEDKSPGASSDPGTTEPISRRTFGQTLGIAALASTVNGLAASAP
                     AAQSGTPAAEALCDLGAVDLVAKIRRREVSARDVMTAHLARIERINPRVNAIVTLVAE
                     RALADAARADEMTVRGGPLGVLHGLPVAHKDLVDTAGIRTTRGSPFYRDTVPTIDAPI
                     VTRIRAAGAITCGKTNTPEFGAGSQTFNSVFGATRNPYDVTKTCGGSSGGAAVALACG
                     MVPIADGSDTGGSLRNPAAYCNVVGIRPSPGRVPGGSGSWSPLPVSGPMARSVADVAL
                     FLSAIAGFDPGSPLSIPEDGSRFRTPLEREFKGVRVAWWRGLGGIPFEPEIRRIIDGH
                     RRTFESVGCVVEEAEPDFTGVDQAFPTLRYAANHPQYAPLVRQRPEWVKDTIKYEVAE
                     AERMTAADYGRALARQSLMYEQSRRFFERYDYFVLPVTQVSPFDVNVPYPTQIAGTPM
                     TTYVDWMRSCWYVSFMSNPAISVPAGFTADGLPVGIQIVGRHRDEWSVLQIAHAFEQA
                     TQFGKTRPGVLKG"
     gene            2174..2623
                     /locus_tag="AC16CY_21410"
     CDS             2174..2623
                     /locus_tag="AC16CY_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR3356424.1"
                     /translation="MTRPRGLVSFMSIDTAYWSLLGADLVLAVHAVFVAFVVFGLALI
                     LIGRALSWPWVRNRVFRLTHLAAIGVVALQAWLGVVCPLTTLEMSLRRRAGASAYEGT
                     FISHWLESLLYYDLPAWVFVVCYTAFAALVGASWFVVPPEPRSGRTR"
     gene            2841..4214
                     /locus_tag="AC16CY_21415"
     CDS             2841..4214
                     /locus_tag="AC16CY_21415"
                     /inference="COORDINATES: protein motif:HMM:NF048350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7507 domain-containing protein"
                     /protein_id="HIR3356425.1"
                     /translation="MARLFLAAATAVLIAAVACSSVSPTSPSGTGATITGTVIASPSA
                     ANVAVAGTPLSAAVDGTGSFFLRGVPAGNVQLVFSGSGASSAVSLLNVADQELIEIDV
                     AITGGTATVQREVRGSSGKVVLCHHTGNGSYHTIDVSVNAEPAHRGHGDAKPGEPVPA
                     DRTKVFSRDCRLISPVEIKKFTNGQDADVEPGPTVAVGAPVNWTYVVTNNSTLTLTSV
                     TVTDDRGVVVTCPQMLPAPGASITCNASGVAVAGQYRNVGTVTATASGMSFTDSDASH
                     YHGGPVSSVTIDKFTNGQDVTQAPGPVIPVGDPVTWTYVVTNRTALALSSLTVTDDRG
                     VAVACPQMLPAPGASITCNASGVAVAGQYRNVGMVVATAGASKFTDSDESFYHGGPIV
                     EEPKVTLCHRTGNGSYHSITVGASAEPAHRAHGDAKVGEPVPGSPGQVFTASCGLQPA
                     GPTSSAG"
BASE COUNT          771 a         1557 c         1512 g          791 t
ORIGIN      
        1 gggccacgca tggatgcctc cctgaaagac cggagccgtc gatgcgccgc ggtgcggtgt
       61 agggtaactc agacggccgg ccagggccag cgtccatgcg gcagctcgag cgctgcgtag
      121 agatcccagg aggagcccgg gagcctggcg cagagcatct acgctggcaa ggaaaaatgc
      181 ggattctcga tcaacccgat caggttgccg aacgggtcgg ccacggttgc gaccttgatg
      241 ctctctccta cgtcgtgcgc cggtaccacg accgatgcgc cggcgctcac gaagtgccgg
      301 acggcctcgt cgattccggc aacccgccag taggcgacgc ttccgccagg tccggccttg
      361 ccggacttct ggtccgggtc gaggcccagc tcgtagccag cgatgttgaa tccgacgtag
      421 aacggctcgt cgaagtacgg cgcctgctga aacgccgccg tataccaggt cttcgcgcgc
      481 tccaggtccg tcaccttgta gatggtcgtc gcaagccctt tgatcatgtt ccagcctcga
      541 actcgcctcg tcaccccttc agcacacccg gccttgtctt tccgaattgc gtggcctgtt
      601 cgaacgcgtg cgcgatctgc agcacgctcc actcgtcgcg gtgacggccc acgatctgga
      661 tgccgacggg cagcccatcc gcggtgaacc ctgcgggcac ggaaatcgcg gggttcgaca
      721 tgaagctcac gtaccagcac gagcgcatcc agtccacata cgtcgtcatc ggcgttcccg
      781 caatctgcgt gggatatggc acgttcacgt cgaatggcga cacctgtgtc acgggaagga
      841 cgaaatagtc gtagcgttcg aagaagcgcc ggctctgctc gtacatcagc gactgccggg
      901 caagcgcccg cccgtaatcc gcggcggtca tgcgctcggc ctctgcgacc tcgtatttga
      961 tcgtgtcctt cacccactcc ggccgttgac ggaccaacgg tgcgtattgc ggatggttcg
     1021 ctgcgtatct gagcgtggga aaggcctgat ccacgccggt gaaatcgggc tccgcttcct
     1081 cgacaacgca gccgacgctc tcgaacgtgc gccgatggcc gtcgatgatg cgccggatct
     1141 ccggctcgaa gggaatgccg ccgaggcccc gccaccacgc gacccgcacg cccttgaatt
     1201 cgcgctcgag cggtgtacgg aagcggctgc cgtcctcggg gatcgagagc gggctcccgg
     1261 gatcgaatcc ggcaatggcg ctcaggaaga gcgcgacgtc cgcgaccgac cgtgccatcg
     1321 ggcccgacac cggcagcggc gaccaactgc cggagccgcc aggtacgcgc cccggcgacg
     1381 gccggatccc gacgacgttg cagtacgccg ccggattgcg gagggatccg cccgtgtcgc
     1441 ttccgtccgc gatcggcacc atgccgcacg ccagcgcgac ggcggcgccg ccgctgctac
     1501 cgccgcaggt cttcgtgacg tcgtacgggt tgcgcgtcgc tccgaacaca ctgttgaagg
     1561 tctgcgatcc tgcgccgaac tccggcgtgt tcgtcttgcc gcacgtgatg gcgccggctg
     1621 ctcggatgcg cgtcacgatc ggtgcgtcga tcgtcggcac agtgtcccgg taaaaggggg
     1681 atccgcgcgt cgtgcgaatg ccggcagtgt ccacgagatc cttgtgggcc accggcagcc
     1741 catgcagcac gccaagcggc ccgccgcgca cggtcatctc gtcggcccgg gcggcgtcag
     1801 ccagcgcgcg ctcggcgacg agcgtcacga tcgcgttcac acgagggttg atccgctcga
     1861 ttctggcaag atgggcggtc atcacgtcgc gggcggagac ttcccggcgc cggatcttcg
     1921 ccacgagatc gaccgcgccg agatcgcaga gggcctcggc agcgggtgtg ccgctctgcg
     1981 ccgccggagc ggaagcggcg aggccgttca ccgtactcgc gagggccgcg atgccaagcg
     2041 tctgaccgaa ggtccggcga ctgatcggct ccgtcgtgcc cgggtcggag ctcgcaccgg
     2101 gcgacttgtc ctccatacgg tgccacagta caccacacgg aaccacttcg cacgcaacgc
     2161 tcgccggacc acaatgacac ggccgcgagg cctggtgtct ttcatgtcga tcgatacggc
     2221 gtactggtct ctgctcggcg ccgatctcgt actggccgtg cacgcggtgt tcgtggcgtt
     2281 tgtcgtgttc gggctggcct tgatcctgat cggcagggcg ctttcctggc cctgggtgcg
     2341 aaaccgggtg tttcgcctca cgcacctcgc ggcgattggc gtcgtcgcgc tgcaggcatg
     2401 gctcggagtc gtatgccccc tcacgacgct cgaaatgtct ctgcgccggc gcgcgggcgc
     2461 ctcagcgtac gagggcacat tcatctcgca ttggctggag tcgctgctct actacgatct
     2521 gcctgcgtgg gtgttcgtgg tgtgctacac cgcgttcgcg gccctggtcg gggcgagctg
     2581 gttcgtcgtg ccacctgagc cgcgcagtgg aagaacgcgg tgagtagaat cttcacaact
     2641 gtcggattcc tgttcactca aaaggccatc ttgtggagac gtaaagtctc cttctgacgc
     2701 ggcttccact ggttgatcga tgtagcgaga aagaccctcc ggattcggct tgaatcctcc
     2761 cgactccatg gtgagtggga cccgtcggtg cgttctcgct tgttgagtcg gaaggcgcac
     2821 ggtttgcttg acggctgtgc atggccagac tcttcctcgc tgccgccacc gctgtcctca
     2881 ttgctgccgt cgcctgcagc agtgtgtcgc cgacctcccc ctcaggaacc ggcgccacca
     2941 ttacggggac ggtcatcgcg tcgccctccg cggcgaacgt ggccgtggcc ggcacgccgc
     3001 tgtcagcggc cgtcgacgga accgggagct tcttcctgcg gggtgttccg gcgggaaacg
     3061 tccagctggt gttcagcggc tcgggcgcga gttcggccgt ctcactcctg aacgtggcag
     3121 atcaggagct catcgagatc gacgtcgcca tcacgggggg cacggcgacg gtgcagcgcg
     3181 aggtgcgtgg aagcagcggc aaggtggtgc tctgccatca caccggcaat ggcagctacc
     3241 acacgatcga cgtcagcgtg aacgccgagc cggcgcaccg cgggcacggt gacgcgaagc
     3301 ctggcgaacc ggtgcccgcc gatcggacca aggtattcag ccgggactgc cgtctgattt
     3361 caccagtcga aatcaagaaa ttcaccaacg gccaggacgc ggacgtggaa ccggggccca
     3421 cggtcgccgt cggtgcaccc gtgaactgga cgtacgtggt gaccaacaac agcacgctca
     3481 ccctcaccag cgtgacggtg actgacgacc gtggcgtcgt cgtgacctgc ccgcagatgc
     3541 tgcccgcgcc tggcgcgtcg atcacctgca acgcgtcggg agtggccgtg gcgggtcagt
     3601 accgcaacgt cggaacggtc acggccacgg ccagcggcat gtcgttcacc gactcggacg
     3661 ccagtcacta ccacgggggc cctgtcagca gcgtgacgat cgacaagttc accaacggcc
     3721 aggacgtcac tcaggcgcca ggtccggtga ttcccgtcgg ggacccggtg acatggacgt
     3781 acgtcgtcac caaccggacg gccctggcgc tctcgagcct gacggtgacc gacgaccgtg
     3841 gcgtcgccgt ggcctgcccg cagatgctgc ccgcgccggg cgcgtcgatc acctgcaatg
     3901 cgtcgggcgt cgcggtcgcg ggacagtacc ggaacgtcgg aatggtggtg gcaacggcgg
     3961 gcgccagcaa gttcaccgac tcggacgaga gcttctacca tggcggaccg attgtcgagg
     4021 aaccgaaggt cacgctctgc catcgcacgg gcaatgggag ctatcactcg attaccgtag
     4081 gcgccagcgc cgaaccggcc catcgggctc acggggacgc gaaggtgggc gagccggtgc
     4141 ccggcagccc cggccaggtc ttcacggcgt cctgcggcct acaaccggcc ggaccgacca
     4201 gttccgcggg ctgagctacg ggtgccagat cgagacgcac gcatccctga gctttggatc
     4261 agaaagctgg tgcgcgttct gccattcgaa accgtattga gaaaccgcca tgacgcagca
     4321 tgaggtgagc gtggccgcag agtagccgta gagaaatcgc ctgcctgtca taatccctcg
     4381 gtcccgagtc ctgacatcga gcccgcgctt ccgtcaacca ccgggagcgt atatagtctc
     4441 tggcagcgga gcacccgatg acacacagaa tcgctgcact cgcgctaacg ctcttcgtcg
     4501 ccgggctcat gccggcacgc gcactcgccc agtcgggctc gcctgcgccg gccgccactg
     4561 cagcgccgta cttcgcggag aactcaatga acgtcttccg ccgcttcaac ggcgacggcc
     4621 gcaagacgct g
//
LOCUS       DBNGSH010000935         2304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_802738, whole genome shotgun sequence.
ACCESSION   DBNGSH010000935 DBNGSH010000000
VERSION     DBNGSH010000935.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_802738"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC16CY_21420"
     CDS             complement(<1..202)
                     /locus_tag="AC16CY_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR3356426.1"
                     /translation="MSRRFKQLETNVVRAGEPERRIEGAVTMPIFQSAMFEYAGETSY
                     HDLKYIRLNNTPTHVVLHAKLAA"
     gene            536..1201
                     /locus_tag="AC16CY_21425"
     CDS             536..1201
                     /locus_tag="AC16CY_21425"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3356427.1"
                     /translation="MMSLRGVEKSYPVGAGQIFVLRRINIDIKPGEFVSIMGPSGAGK
                     STMLHILGMHDSAWAGEYHLMGHPIHALPQKDRATLQKRHIGFVFQSYHLLDNLTVYE
                     NIELPLSYRDVRKSERQGTVASVLDRFGIVAKKDLYPNQLSGGQQQLVGVARAIVANP
                     AVILADEPTGNLHSSQGQEIMELFKRLNEQGTTIVQVTHSELNASYGDRIIKLKDGYV
                     EQS"
     gene            complement(1213..2091)
                     /locus_tag="AC16CY_21430"
     CDS             complement(1213..2091)
                     /locus_tag="AC16CY_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356428.1"
                     /translation="MPSRSATAIGVLVVCLCAAVAPAQDRPLPDQEAFLRETRRHLQT
                     DATLQSSYMYVETRREQELDGKGRVSEDRVRVYENYPGLPGQPRWERLIAENGRPVSP
                     EKLAEQDRDRQQDAGEAARRLTNQPADERARLERQRERQLREMERAIDDIMTVYEIRM
                     VGREAIEGHDTIAFSLTPRPDAKPKTREGSQMKKFSVKAWISESDYELVRLEAKAIDS
                     LSWGLGLLARLHKGAQLTFLRRKVNGEVWLPAMFNYSGSARVGLVWTLRRSGASEYSG
                     YRKFSVDTSTTFNTPK"
BASE COUNT          382 a          786 c          714 g          422 t
ORIGIN      
        1 tcgcggcgag cttggcgtgc agcaccacgt gcgtcggcgt gttgttgagc cggatgtact
       61 tcagatcgtg gtagctcgtc tcgcccgcgt attcgaacat cgcggactga aagatcggca
      121 tcgtgaccgc gccttcgatc cgccgctccg gctcgccggc ccggacaacg ttggtttcaa
      181 gctgtttgaa acgacggctc atgcccgcct cccactctaa ccaccaacca ccaacctaag
      241 tggaggatac cccctccgcc ggccgggttg tgcgtatgcc attagcccag tccaaggaaa
      301 tcgggttgga agttggagat tgggcgttgg gagttggtgc cggtgtccgc ccgcgggacc
      361 ggcgttcccg tcttcgaaca catccagcgc cggatcgcgt aaattcatta gacacgcgtc
      421 cggcacacgg attgattccg ccggcctgtc gagactggac acgaacacta tgatcttcag
      481 acgctcatcc tcgtccgcgc tgaccgagcc tcccccgctc gacccgaaca cccccgtgat
      541 gtcgctccgc ggcgtcgaga agtcctatcc ggtcggggcc ggccagatct tcgtgctccg
      601 ccgcatcaac atcgacatca agccgggcga gttcgtctcg atcatggggc cgtcgggcgc
      661 gggcaagtcc acgatgctcc acattctcgg gatgcacgac agcgcgtggg cgggcgagta
      721 ccacctgatg gggcatccga ttcacgccct gccgcagaag gaccgcgcga cgctgcagaa
      781 gcgccacatc ggcttcgtgt tccagagcta tcacctgctc gacaacctga cggtctacga
      841 gaacatcgag ctgccgctct cctatcgcga cgtcaggaag tcggagcgcc agggcacggt
      901 cgccagcgtg ctcgaccgct tcggcatcgt ggccaagaag gatctctatc ccaatcagct
      961 gtcgggcggg cagcagcagc tggtcggcgt ggcgcgcgcc atcgtcgcca accccgcggt
     1021 gatcctcgcc gacgagccga cgggcaacct gcattcctcg caggggcagg agatcatgga
     1081 gctcttcaag cggctcaacg agcaggggac gacgatcgtc caggtgacgc actccgagct
     1141 gaacgcgtcg tacggagacc ggattatcaa gttgaaagac gggtacgtcg aacagtcctg
     1201 accagtgtcg cgctacttcg gggtattgaa cgtcgtggac gtatcgaccg agaacttgcg
     1261 gtagcccgaa tactccgacg cgccgctccg tcgaagcgtc caaacgagtc cgacgcgcgc
     1321 gcttccgctg tagttgaaca tcgccggcag ccagacctcc ccgttcacct tgcgacgcag
     1381 aaaggtcagc tgcgcgccct tgtgcaggcg cgccagcagg ccgaggcccc aggagagcga
     1441 gtcgatggcc ttcgcctcga gccgtaccaa ctcgtaatcg gactcgctga tccacgcctt
     1501 cacgctgaac ttcttcatct ggctcccttc gcgggtcttc ggcttcgcat cgggccgcgg
     1561 cgtgagcgag aaggcgatcg tgtcgtggcc ttcgatcgcc tcacgaccga ccatgcggat
     1621 ttcgtacacg gtcatgatgt cgtcgatggc ccgctccatc tcgcggagct gcctctcccg
     1681 ctggcgctcc aggcgcgcgc gctcgtccgc gggctggttg gtcagacgtc gagcggcctc
     1741 cccggcatcc tgctgccggt cccggtcttg ctccgcgagc ttctcgggag acaccggacg
     1801 cccgttttcg gcaatgaggc gctcccaccg gggctggccg ggaaggccgg ggtagttctc
     1861 gtagacccgc acgcggtctt cgctgacgcg gcccttcccg tcgagctcct gctcgcgccg
     1921 cgtctcgacg tacatgtagc tgctttgcag cgtcgcgtcg gtctggaggt gcctgcgggt
     1981 ttcgcgaaga aaggcctcct ggtcgggcag cggacgatcc tgtgccggcg caacggccgc
     2041 gcacaggcat accaccaaca cgccgatggc tgtcgcactg cgggatggca tcctgagatc
     2101 tatcgtaacc gcgtcccgcg cgcggacgca gcgtcccacg atcggacaac ctgtgccgga
     2161 acaggcactt tttccccgcc tcctggacgg cacgccgatt gatgtgcttc tcggccgggc
     2221 ctgagtccaa tggagtccgt gcttcgggac ctccgcatcg ccgcccgcgg gcttcggcgc
     2281 aacctcggct tcacggccgt ggcc
//
LOCUS       DBNGSH010000936         2587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1991414, whole genome shotgun sequence.
ACCESSION   DBNGSH010000936 DBNGSH010000000
VERSION     DBNGSH010000936.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1991414"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..1528)
                     /locus_tag="AC16CY_21435"
     CDS             complement(344..1528)
                     /locus_tag="AC16CY_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158044795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HIR3356429.1"
                     /translation="MVSRVGPRLELADIVRTHGDAYRRTHRLAPVQHRALRAIETCRT
                     AVLGGHQETCDQCGAVRISYNSCRNRHCPKCQTLTKERWLAARRADLLPIPYFHIVFT
                     LPHALNALTQGNPRVIYTLLFRAAADTLLTFGRDPRHLGGTIGITAILHTWGQNLSQH
                     LHLHCLVTGGALAADGSRWIAGRSSFLFPVRALSTVFRAKYLAGLQHAGAAGQLTFAG
                     GTADLLDPPVFRRFLGQLRAVDWIVYAKRPFAGPEQVLDYLGRYTHRVALSNDRLVHH
                     RDGQVGFRWKDYADHNRVKVMALEADEFLRRFLVHVVPRGFMRIRHFGLLANRTRRDR
                     LRRCRLLLHHPPSADAGPESVADMMQRLTGVDLSRCPICGDGRMHVTAILLRAPSPPD
                     TS"
     gene            complement(1557..1823)
                     /locus_tag="AC16CY_21440"
     CDS             complement(1557..1823)
                     /locus_tag="AC16CY_21440"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3356430.1"
                     /translation="MHPTSLQRAYQAAKRRAGVTKPGGIHGLRHAFATHLLEAGVDLH
                     TIQRLLGHGDITTTTRYFHLTKHAQIGPNSALDLLARFTPPPQG"
     gene            complement(1904..2413)
                     /locus_tag="AC16CY_21445"
                     /pseudo
     CDS             complement(1904..2413)
                     /locus_tag="AC16CY_21445"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          493 a          775 c          907 g          412 t
ORIGIN      
        1 gctaacggaa agggtttcat ccgcactgcc acggcgatcg acgtgagccc cgacgcgggc
       61 tcagtgcgga taaacccggt tggactgagc cgcgtggcga tgctgccgca agtctatggg
      121 gattgtaagg cagtgcgggt gtgagagggc gggcattcga ccatcgcgac gatgcgtgga
      181 gcgtgacgga cgcggcgcgg cgcccgtgac agcgcacaga gaggaggacg ttcagggtat
      241 ctccgtgccg tcgatacgac tccggtcaga tgcgaggcgc agcagggtcg accggacgcg
      301 tgtccgtcga gaagcgtcgt ccggtggcga cacctgccgg gcctcatgag gtatccggcg
      361 gggagggcgc ccgcagcagg atggcggtga cgtgcatccg gccatccccg cagatcggac
      421 agcgcgacag gtccacgccg gtgagccgct gcatcatgtc agcaacggat tcagggccgg
      481 cgtcggcgga cggtgggtgg tgaagcagca gacgacaccg tctgagcctg tcgcgacggg
      541 tgcgattggc gagcaggcca aagtgacgaa tgcgcatgaa gccgcgcggg acgacgtgca
      601 cgagaaagcg tcggaggaat tcgtcggcct caagcgccat caccttcacg cgattgtgat
      661 cggcatagtc tttccaacga aatcccacct gaccatcacg atgatggacg agccgatcgt
      721 tcgagagggc gacgcggtgc gtgtaccgcc ccaggtagtc gagcacctgc tccgggccag
      781 cgaagggccg ctttgcgtac acgatccaat ccacggcgcg cagctgaccg agaaagcggc
      841 gaaagacggg agggtcgagc agatcggcgg tgccaccggc gaaggtgagc tggccagcgg
      901 cacccgcgtg ttgcagcccg gcgagatatt tcgcacgaaa gaccgtcgac agggcgcgga
      961 ccggaaacag gaaggacgat cgaccggcga tccaccgcga gccatcggcc gccaaggcgc
     1021 caccggtcac caggcaatgg aggtggaggt gctgagatag attctggccc caggtgtgca
     1081 gaatcgccgt gatgccgatc gtgccgccca ggtggcgcgg gtcccggcca aacgtgagga
     1141 gcgtgtcggc agccgcgcga aacaggagtg tgtagatcac ccggggattg ccctgcgtga
     1201 gcgcgttcag ggcgtggggc agcgtgaaga cgatatggaa atacggaata ggtaacaggt
     1261 cggcacggcg tgccgccagc cagcgttcct tcgtcagcgt ctggcacttg gggcaatgtc
     1321 ggttgcgaca cgagttgtac gagatgcgca ccgcgccaca ctggtcacac gtctcctgat
     1381 gcccgccgag cacggcggtc cgacaggtct cgatcgcgcg gagcgctcga tgctggacgg
     1441 gggcgagacg gtgcgttcga cgatacgcgt cgccatgggt gcgcacgatg tccgccagct
     1501 cgagtcgagg accgacgcgc gagaccaccg tgcccgatgg cacggcgcac accgaatcag
     1561 ccctgcggcg gcggcgtgaa gcgcgcgagc agatcgagcg cggaattcgg cccgatctgg
     1621 gcgtgcttgg tcaaatggaa ataccgggtc gtggtcgtga tatcgccgtg tccgagcaac
     1681 cgttgaatcg tgtggaggtc gacgccggct tccagcaggt gcgtggcaaa ggcgtgacgg
     1741 agtccgtgaa tcccacccgg cttggtgacc ccggctcgcc gtttcgcagc ctgatacgca
     1801 cgctgcaaac tggtcggatg catcggtcgg tcgccccgtc cgcaggaaag agccacagcc
     1861 gggggcgaga ggtcggccaa tacacgcgca agcactccaa caagcgagcg gagaggatgg
     1921 tgtaccgatc cttcgcgcct ttcccctgtt ccacgcgaat catcatccgc gtcgcgtcga
     1981 tgtcgcgcac ccgcagatgg aggacttcat tgaggcgcaa gcccgccgca tacgtggtga
     2041 gcagcatcgt ccgatgtttc ggactgcgag tctgcgcgag gacccgctcg atctcgtcgg
     2101 ggctcaggat gactgggagc tgaaccggct ggcggggccc gggaatctca aaggtcgtgc
     2161 gatcgcgctt cagggtcgtg tgatagaaga accggaggcc gtgcacgaca atgtggagcg
     2221 tgctccagga gcggtgtcga tcgcgcagca gatggcgtaa gtacgcttgc acctcctgat
     2281 cggagatctg gtcaggcgag cgccggtagt agcgcgcgag cccttcgacc gcccagaggt
     2341 acgtttcacg agtccggtcc gccatcccgc cgaccaccat gtcttcgtcc atgcgacgac
     2401 gcaattcagt catgacacac ctcgctcccg cgaacgtctt ccgtgccgtt cgtcagcgcg
     2461 cattgtgtgg gcagcgtctt cgtcgcgagc gaacaccaga ggagtggccg acgccacggc
     2521 cactgcagta tcgatggagg taccttggag ggtctttcct gccgcgttca gcggcttagt
     2581 tcaacta
//
LOCUS       DBNGSH010000937         3613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1821309, whole genome shotgun sequence.
ACCESSION   DBNGSH010000937 DBNGSH010000000
VERSION     DBNGSH010000937.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1821309"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="AC16CY_21450"
     CDS             complement(<1..880)
                     /locus_tag="AC16CY_21450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HIR3356431.1"
                     /translation="MLLVVSLAGVTDSFGFGSEGHQIVCEIAFNRLTPGGRALVNAVR
                     ADVGDVTDPFDSCPGCQAAHPDDGRNLTFRQGCIWADEARTDTFKGTYEYHFINVSRE
                     FTTLDLARDCASLDCVAVAIQRYARYIALPRSTNRRERERRVLALRFLGHFVGDLHQP
                     LHVGFAQDQGGNLIDVRASSGADTRSLHSVWDEEVLQRAGLTDPVLDGAALNKEITPA
                     ELTAWQTFDVVAWAAESFALARTRAYTKPDGTRVMPNDRLTDAYFKAASPVLREQLKK
                     AGVRLAHLINAAAAGTL"
     gene            complement(919..2127)
                     /locus_tag="AC16CY_21455"
     CDS             complement(919..2127)
                     /locus_tag="AC16CY_21455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3356432.1"
                     /translation="MNTLSREGIGADAIDGVVPRHVVRPESPDELSRALAGASRDRHS
                     TVLRGGGTKIEWGRSPASVDLVVETAGLARLVAHRHGDMTATAQAGMPLMLLNRHLAE
                     QGQYLPVDSAFHGATIGGMIATNDCGPLRHRFGTPRDLLIGITLAMADGRLVKAGGTV
                     VKNVAGYDLGKLVSGSFGTLAAIVDATFKLLPLPRASRTLVSTYMDGSALARDVAALN
                     ASQVELTSFDVHVSDKARFQLLMRMASSGEATEAMAAEASRLLSSPASILTGDDEQAF
                     WAEQVRAPWSEGGTIVRLSWLPANLSVVLASLDELRRHGCRPSMFTGRTIGAGLLRLE
                     GDDRACAAAITDLRSRSEVGHVVVLRASRRLKEQTDVWGGGSDRTGPARALKRMFDPQ
                     GILNAGRGPI"
     gene            complement(2306..>3613)
                     /locus_tag="AC16CY_21460"
     CDS             complement(2306..>3613)
                     /locus_tag="AC16CY_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3356433.1"
                     /translation="SAADVQAIVRLCSREGLPFVARGHGTGLSGGALPVPGGVVISLA
                     RLNRVLDVDIANRRVTVEPGVTNLEITRQVAPFGYYYAPDPSSQQVCSIGGNVAENSG
                     GAHCLKYGFTVHHILAVEAVLPDGELVQFGSPLADAPGPDVLGLIVGSEGTLAVVTKA
                     VVRILRKPESVQTLLAAYDSIGEAGSAVSEIIKAGIIPAAVEMMDRLAIQAAEAAVHP
                     NFPDADTILIVELDGPAAEVRELFAIVEATCRKTGATRIEVAQDEAQRARIWKGRKAA
                     FAAIGRVSPNYYVQDGVVPRTKLPEVLERIRLLESREGLRIGNVFHAGDGNLHPLICF
                     DERIPGQAALAERVAGEILIYCIEAGGSITGEHGVGADKTTFMPKMFSSDDLDVMQSV
                     RDAFDPKRLCNPGKVLPTPRLCGEVPGPYRQHPVERAGLAERF"
BASE COUNT          594 a         1244 c         1178 g          597 t
ORIGIN      
        1 gcagcgtgcc ggcggccgcg gcattgatga gatgcgccag ccgcacgccg gccttcttga
       61 gttgttcccg cagtaccggt gacgcggcct tgaagtacgc atccgtgagc cgatcgttcg
      121 gcatgacgcg cgtcccgtcg ggcttcgtgt aggcgcgggt gcgtgccagc gcgaacgact
      181 cggccgccca cgccaccaca tcgaaggtct gccatgcggt gagttcagcc ggcgtgattt
      241 ccttgttcag cgcggcgcca tccagaacag ggtcggtcag tccggcccgc tgcagcactt
      301 cttcatccca cacgctgtgg agacttcgcg tgtcggcgcc gctgctcgcg cggacgtcga
      361 tgaggttgcc tccctggtcc tgggcgaagc cgacgtgcag cggttggtgc agatcgccca
      421 cgaagtggcc aaggaaacgc aaggccagca cgcggcgctc gcgttcacga cggttcgtgg
      481 aacggggaag ggcaatgtag cgggcgtacc gctgaatcgc gacggcgacg cagtccagcg
      541 acgcgcagtc gcgggcgagg tcaagcgtcg tgaactcgcg cgacacgttg atgaagtggt
      601 actcgtaggt gcctttgaac gtgtcggtgc gcgcttcatc cgcccagatg cacccctgcc
      661 gaaacgtcaa attgcgccca tcgtcggggt gggcggcctg acagcccggg caactgtcga
      721 agggatccgt gacgtcacca acgtcggccc ggacggcgtt cacgagggcg cggcctccgg
      781 gtgtgagtcg attgaacgcg atctcacaaa cgatctggtg gccctcgctc ccgaacccga
      841 acgagtccgt cacgccagcg agcgagacga ccaggagcac agcacacgta gtcaggcagc
      901 gggagtgcga catggccatc aaattggtcc ccttccggcg ttgagaatgc cctgcggatc
      961 gaacatgcgc ttcaacgcgc gcgctggccc ggtccggtcc gatccgccgc cccagacatc
     1021 ggtctgctcc ttcaggcggc gggatgcacg cagcacgacg acgtgtccga cctcacttct
     1081 cgaacgcagg tccgtaatcg cggcggcaca cgcgcgatcg tctccctcga ggcgcagcaa
     1141 ccccgcaccg atcgttctcc cggtgaacat cgacgggcgg cagccgtgcc ggcgaagctc
     1201 gtcgaggctg gcgagcacga cggacaggtt ggccggcagc cagctcagtc tcacgatcgt
     1261 tccgccctcc gaccatggcg cgcgcacctg ctcggcccag aacgcctgct cgtcgtcccc
     1321 ggtcaggatc gacgccgggc tcgacagcag tcgagacgct tcggcagcca tcgcctcggt
     1381 cgcttccccg ctcgacgcca tccgcatcag gagttgaaat cgcgccttgt ccgagacgtg
     1441 cacgtcgaac gacgtcaact ccacctggct tgcattcagg gcggcaacgt cgcgcgccag
     1501 agcgctgccg tccatgtagg tggacacgag cgtccgcgac gcgcgcggga gcggcagcag
     1561 cttgaaggtg gcgtcgacga tcgctgcgag cgtgccgaac gaaccgctga cgagctttcc
     1621 aagatcgtaa ccggccacgt tcttcacgac cgtcccgccg gccttgacga ggcggccgtc
     1681 ggccatggcg agcgtgattc cgatcaacag gtcccgcgga gtgccgaagc gatggcggag
     1741 cggcccgcag tcgttggtcg caatcatccc gccgatcgtg gcgccgtgaa acgcactgtc
     1801 tacaggaagg tactgaccct gctcggcgag gtggcggttc aacagcatga ggggcatgcc
     1861 tgcctgcgcc gtcgccgtca tgtcgccgtg tcgatgcgcc accagccgag cgaggcccgc
     1921 cgtctcgacg acgaggtcca ccgacgcagg cgaacgcccc cattcgatct tcgtgccgcc
     1981 cccgcgcagc acggtactgt gccggtcccg cgacgcgcca gcgagcgcgc ggctcaactc
     2041 gtccgggctc tccggccgca cgacatggcg cgggacgaca ccatcgatgg cgtccgcccc
     2101 gatcccctca cgagacagtg tgttcacgct tctctcttct cgcttctcag ttcgtgttca
     2161 cgttcacgtt ctgttcggcg ttcgtaattg agcgtgcgac gttccaaccg ggaaagcggg
     2221 ggcggtcgga gcctgaacac gaaccgagaa gcgagaagac tgaagtgtga acgacagatc
     2281 gcattctcgt ggcgcgaacc tcgttttaga accgctcggc gaggcccgct cgttcgactg
     2341 gatgctgccg gtacggaccg ggaacttcgc cgcacagcct gggcgtaggc aggaccttgc
     2401 ccgggttgca caaccgtttc ggatcgaacg cgtcgcggac gctctgcatg acgtccaggt
     2461 cgtcgctcga gaacatcttc ggcatgaacg tcgtcttgtc cgcgccgacg ccgtgttcgc
     2521 cggtaatcga ccccccggcc tcgatgcagt agatcaggat ctcgccggcg accctctccg
     2581 ccagcgccgc ctgtcccgga atgcgttcgt cgaagcagat gagcgggtgg aggttgccgt
     2641 cgcccgcgtg gaaaacgttt ccgatccgca ggccctcccg cgattcgagc aggcggatcc
     2701 gttcgagcac ttcgggcagc ttcgtacgcg gcacgacgcc gtcctgcacg tagtagttcg
     2761 gcgagacgcg cccgatcgcg gcaaacgcgg ccttgcggcc cttccagatg cgggcacgct
     2821 gcgcctcgtc ctgcgcaacc tcgatgcgcg tcgccccggt cttccggcac gtcgcctcga
     2881 cgatcgcgaa cagctccctg acctccgcgg ccgggccatc gagctccacg atgagaatcg
     2941 tgtccgcgtc cgggaagttg gggtgcaccg cggcctccgc ggcctgaatg gcgagccggt
     3001 ccatcatctc caccgccgcc gggatgatgc ctgccttgat gatctcggaa acggccgatc
     3061 ccgcttcccc gatgctgtcg taggcggcga gcagtgtctg cacgctctcc ggcttgcgga
     3121 gaatcctgac gaccgctttc gtgacgacgg ccagcgtgcc ttcgctgccg acgatgagcc
     3181 ccagcacgtc cgggcccggc gcgtcggcca gcgggcttcc gaactgcaca agctctccgt
     3241 cgggaagcac ggcctcgacc gcgaggatgt gatgaacggt gaatccgtac ttcaggcagt
     3301 gggcgccccc cgagttctcg gcaacgttgc cgccgatgga gcacacctgt tgactggagg
     3361 gatcgggcgc gtagtagtag ccgaagggcg ccacctgacg cgtgatctcc aggttggtca
     3421 cgccgggttc caccgtcacg cggcggttgg cgatgtcaac gtcgagcacg cgattcagcc
     3481 tggccaggct gatgacgacc ccgcccggga ccggcagcgc gccgccggac agtcccgtcc
     3541 catgcccccg cgcgacgaag ggaaggccct cgcggctgca cagccgcacg atggcctgga
     3601 cgtcggccgc cga
//
LOCUS       DBNGSH010000938         4155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1482981, whole genome shotgun sequence.
ACCESSION   DBNGSH010000938 DBNGSH010000000
VERSION     DBNGSH010000938.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1482981"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="AC16CY_21465"
     CDS             complement(<1..794)
                     /locus_tag="AC16CY_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356434.1"
                     /translation="MRTALPAVLAVLTLYAGVGGHAQDAGVQDLVSRAARYVEEYERQ
                     LSAVVSEERQTQRVIKRDGTTGKKRELVSDLLMVKTGDYTQTFRDVIAVDGKPVRNRQ
                     ERLQKLFLGEPHGRMKQAQAIAKESARYNIGFERGLDTLMLPLIILHAKRATGFRFAR
                     TADGLTFDEFRSPALLRSRIRGDVRDMFLRGALTIEDDTGRLGGARLVAENPQFTVTV
                     DVRYVEEAASGLLVPVELREDYRTPVEKDRLEVLATYSNFRRFQVTV"
     gene            1003..3654
                     /locus_tag="AC16CY_21470"
     CDS             1003..3654
                     /locus_tag="AC16CY_21470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3356435.1"
                     /translation="MALIGEGGMGQVYRATDTRLKRQVAIKTLPPSLSADQDRLARFQ
                     REAEVLASLNHPNIAAVHGFEESGGVSALVMELVDGDDLSLRIARGAIPLEEALPIAR
                     QIAEALEAAHEKGIIHRDLKPANIKLRADATVKVLDFGLAKALEPAGAPSGSATMSPT
                     INTPAMTQAGMILGTAAYMSPEQAKGRAIDRRADVWAFGAVLFEMVSGRQPFSGDTVS
                     ETLAEVMKSEPPWQAIPVTVPAHLVRLIRQCLVKEPRQRIRDMGDVRLALDGTFESAT
                     PVTTLSPDVPWTRLAAMRLAAAAAVVASIGVGGAVWMMARPAPAPTAAIARLAMTLPD
                     DQRVGGLGLDQLALAVSPRGTLVAYVAVMSGREQLYVRAIDAVESKALAGTEQATNPF
                     FSPDGQWIGFFSQGKLKKVSVAAGTIQTLSDAPNARGGSWAGDVIYFAATNSGGISKV
                     SADGGVPTEVTTLDRAKGEISHRWPQVLPGAQALLLDVWTGPGADEKQVHVLRLDTGK
                     RTVVVQAAASGRYVASGHVIYARNDELFSVPFDVDGLRVSGQASRLRDTAWKGSEGNQ
                     FAVSDNGVFVSVSGSASRYERRLVWVGRDGRVEPLAAPPREYYGNAVISPDGTRAAVD
                     MEGGTVSVWLYDFVRATLTPLTTGKGSSQAPRWTADGTRLVYRGTRTGTRNLWWKGVD
                     DAAGEERLSSGDGIQTPGSWSADGQWLAYNDSDPVTGFDIWALPSGGDRKPRAVAGTP
                     SMEASPRLSPDGRWLAYTSDESGRTEVLVQSFPEPGGRTQISTRGGTEPVWSHDGREL
                     FYLNGDAMMAVEIRSSPAFAAGTPRMLFEGRYVRSPNSVASYDVSADGQRFLRVQPMH
                     PDPPTNQIQVTLNWFEELKRIVRREFP"
     gene            3672..>4155
                     /gene="greB"
                     /locus_tag="AC16CY_21475"
     CDS             3672..>4155
                     /gene="greB"
                     /locus_tag="AC16CY_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR3356436.1"
                     /translation="MNGSRHVSQALEKSDKNYITPSGLQRLRDELRFLLARERPAVTQ
                     TVAWAASNGDRSENADYQYGKRRLRQIDRRIRFLTKRIDAAEVVDPESPRAGRAATHV
                     FFGATVRYADADGTERVVSIVGLDEVDLDRHYISWVSPLARALMKSGAGDTVVLSAPG
                     R"
BASE COUNT          722 a         1329 c         1418 g          686 t
ORIGIN      
        1 acggtcacct ggaaacgccg gaagttcgaa taggtcgcca acacttcgag ccggtctttc
       61 tcgaccgggg tgcggtagtc ttcgcgcaat tcgaccggaa cgagcaggcc tgacgcggcc
      121 tcctccacgt atcggacgtc cacggttacg gtgaactgcg gattctcggc gaccagccgc
      181 gcgcctccca ggcggccggt gtcgtcctcg atcgtcaggg cgccgcgcag gaacatgtcg
      241 cgcacgtcac ccctgatgcg ggaccgcaac agggcaggcg agcggaattc gtcgaacgtg
      301 agcccgtctg ccgtgcgcgc gaagcggaag cccgtcgccc tcttggcgtg taggatgatg
      361 agcggcagca tcaacgtgtc gaggccgcgc tcgaacccga tgttgtagcg tgcgctttct
      421 ttggcaatgg cctgcgcctg tttcatccgc ccgtgcggct cgccgagaaa cagcttctgc
      481 agccgctcct gacggttgcg gaccggcttc ccatcgacgg cgatgacgtc tcgaaacgtc
      541 tgtgtatagt cgcccgtctt gaccatcaac aggtccgata cgagctcacg cttcttgccc
      601 gtcgttccgt cgcgcttgat gacgcgctgc gtctggcgct cctcagacac caccgccgac
      661 agctgccgtt cgtactcctc cacgtaccgg gcggcgcggg aaacaaggtc ctgcacgccg
      721 gcatcctgtg cgtgcccgcc aacgccggcg tacagcgtga gcacggcaag gacggctgga
      781 agggcagttc gcatatatac ggcgaggctc cagcggccgc gctttgcgca acccgtgcca
      841 agtcaggcgt acaagaacac gtgcctttca acgcggcgac ctcgcgcccc gcctccgcca
      901 aggcttcggc ggggcgggcc gtcctcctcg caggacgcga cgcgctctat aatcaccaac
      961 ctgaccctca ctccaggcac gcgcgtcggc gtctacgaaa tcgtggcgtt gatcggtgaa
     1021 ggcggcatgg ggcaggtgta tcgcgccacc gatacccggc tgaaacgtca ggtcgccatc
     1081 aagactcttc cgccatcgct ctcggccgac caggatcggc tcgcgcgctt ccagcgtgag
     1141 gcggaagtgc tcgcgtcctt gaatcatccc aatatcgccg ctgtccacgg cttcgaagag
     1201 agtggcggtg tctccgcact cgtgatggaa ctggtcgacg gcgacgatct ctcgcttcgc
     1261 atcgcgcgtg gcgcgatccc gctcgaagag gcgctcccga tcgccaggca gatcgccgag
     1321 gccctcgagg cggcacacga gaaggggatc atccatcgcg atctcaagcc ggccaacatc
     1381 aagctgcgcg cagacgccac cgtgaaagtg ctcgacttcg ggctcgcaaa ggcgctggag
     1441 cccgcgggtg cgccgtcggg gagcgccacg atgtctccga cgatcaacac accggcgatg
     1501 acgcaggcag gcatgatcct gggcacggcc gcctacatgt cgccggagca ggcgaagggg
     1561 cgggctatcg atcggcgcgc ggatgtgtgg gcgttcggag cggtgctgtt cgagatggtg
     1621 tcaggccggc agccgttctc gggcgacacg gtgtcggaga cgctggccga agtgatgaag
     1681 tcggagccgc cctggcaggc catccccgtc actgtgccgg cgcatctcgt gcgcctcatt
     1741 cgacaatgcc tggtcaagga accgcgccag cggattcgcg acatgggcga cgtgcgcctg
     1801 gcgctggatg gcacgttcga gtcagcgacg ccggtgacga ccctgtcgcc cgatgtgccg
     1861 tggacacgcc tggccgcgat gcgtctcgcg gcggcggcgg cggtggtcgc gtcgatcggt
     1921 gttggtgggg ccgtgtggat gatggcgcgt ccggcgcccg cgccaacggc cgccatcgcg
     1981 cggttggcca tgacgctgcc cgatgaccag cgcgtcggtg gcctgggcct ggatcaactc
     2041 gccttagctg tttcgccccg ggggacgctt gttgcgtacg tggcggtgat gagcggccga
     2101 gagcaactgt acgtgcgcgc catcgatgct gtcgaatcga aggctcttgc aggaacggag
     2161 caggccacca atcctttctt ctctccagat gggcagtgga tcggcttctt ctcgcaggga
     2221 aagctgaaga aggtctctgt tgcggccggc acgatccaga cgttgtccga cgcgcccaac
     2281 gcacgcggcg gaagctgggc cggcgacgtc atctacttcg cggcaaccaa cagcggtggg
     2341 atctcgaagg tctcggccga tggcggagtg ccgactgaag tcaccacact cgaccgcgcc
     2401 aagggcgaga tcagccacag gtggccacag gtgctcccgg gcgctcaagc gctgctgctc
     2461 gacgtgtgga cgggccccgg agcagacgaa aagcaggtgc acgtcctgcg gttggatacc
     2521 gggaagcgaa cggttgtcgt gcaggcggcg gcatcaggcc gctacgtcgc atcgggtcac
     2581 gtgatctacg cgcggaatga cgagctgttc tcagtgccgt ttgacgtaga cggcttgcgc
     2641 gtgtccgggc aggcaagccg tctgcgcgac acggcgtgga agggcagcga aggcaaccaa
     2701 ttcgcagtct cggacaacgg cgtcttcgtg tccgtatctg gaagcgcgag ccgatacgaa
     2761 cgtcggctcg tgtgggtggg ccgcgacggc cgcgtcgagc cgctggcggc gccgccgcgc
     2821 gagtactacg gaaacgccgt catctcgccg gacgggacgc gagcagccgt cgacatggaa
     2881 ggggggacgg ttagcgtgtg gctctatgac ttcgtgcgcg ccacgctgac gccgctgacg
     2941 acagggaagg ggagcagcca ggccccgcgc tggactgccg acgggacgcg tctcgtctat
     3001 cgcggaacac ggacgggaac gcggaacctg tggtggaagg gagtggacga tgcggcggga
     3061 gaggagcgct tgtcgagcgg cgacggcatt cagacgccag gatcgtggtc ggccgatggt
     3121 caatggctgg cctacaacga cagtgacccg gtaacgggtt tcgacatctg ggccttgccc
     3181 tccggcggtg accgaaaacc acgggccgtg gcggggacac cgtctatgga agcgtccccg
     3241 aggctctcgc cagatggccg ctggctcgca tacacgtctg acgaatccgg ccgtactgag
     3301 gtgctcgtgc agtcgttccc ggaaccgggg ggcagaacac agatctccac ccgcggcggc
     3361 accgagcccg tctggtctca cgatggacgg gaactgttct atctcaacgg cgacgccatg
     3421 atggccgtcg agattcggtc gtcaccagcg ttcgcggccg gcaccccacg aatgctgttc
     3481 gagggccgct acgtgcgcag tccgaattcc gtcgccagct atgacgtgag cgcggacggc
     3541 cagcgattcc tccgcgtcca gccgatgcat cccgacccgc cgacgaatca gattcaggtg
     3601 acgttgaact ggttcgagga gttgaagcgg atcgtccgcc gcgagttccc atgataacgg
     3661 gactaatggt catgaatggg tcccgtcacg tgagccaggc actcgagaaa tccgacaaga
     3721 actacatcac cccgagcggc ttgcagcggc tcagggacga actccgtttt ctgctcgcga
     3781 gggaacgtcc ggccgtgacg cagacggtcg cgtgggcggc gagcaacggc gaccgcagcg
     3841 agaacgccga ttaccagtac ggcaagcggc ggctgcgcca gatcgatcgc cggattcgct
     3901 tcctcaccaa acgcatcgac gcggcggaag tggtggaccc ggagtcgccg cgagccgggc
     3961 gggccgccac gcacgtgttc ttcggagcga cggtgcgcta cgccgatgcc gacgggacgg
     4021 aacgcgtggt cagcatcgtc ggactcgacg aggtcgatct cgatcgccac tacatcagct
     4081 gggtgtcgcc gctcgcgcgc gcgttgatga agtcaggggc aggcgacacc gtggtgctca
     4141 gcgctccagg ccgta
//
LOCUS       DBNGSH010000939         1978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3520870, whole genome shotgun sequence.
ACCESSION   DBNGSH010000939 DBNGSH010000000
VERSION     DBNGSH010000939.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3520870"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="AC16CY_21480"
     CDS             complement(<1..572)
                     /locus_tag="AC16CY_21480"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3356437.1"
                     /translation="MKAAVPFLVLLTSLATFGQEPAPGAIAGTVQDVSGSPAPGATVT
                     AVRPDGTVAGSVVTGANGSFTLSLPPDTYAIRATLPGFQLASVGGVELRSGSRVDLQP
                     FRLRVGPIPEVLRQAPPMPFGRDREYDITADRQTRQGAVVQYRGNVRMKTYSMEVSAD
                     ELDFDTRARTGDARGNVRVRVVPPEFKMIPL"
     gene            complement(590..985)
                     /locus_tag="AC16CY_21485"
     CDS             complement(590..985)
                     /locus_tag="AC16CY_21485"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3356438.1"
                     /translation="MPVLVLLTSLAVFGQEPAPGVIAGTVLDASDLAIPGATVTAFRQ
                     DETLAGTVVTSSNGSFILSLPSGRYTVRATLQGFRPAGESGIELRSEARVDLRPFRLR
                     VARGDIRFRGPQPEIKMLPLRHDRRRETN"
     gene            complement(1020..1496)
                     /locus_tag="AC16CY_21490"
     CDS             complement(1020..1496)
                     /locus_tag="AC16CY_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356439.1"
                     /translation="MTLPDGALPQAHAALQRLQAWTPPAPPVPTGREAAREWLRDMSE
                     RHQARIQARMSEMRERNPDQCFAVLGGGGGHRGPRGTSSRHSFGYRDGEDDVYETAIV
                     HENHELVLVERRRIDRPARKLVFALQVTGPDGATARLDRTFDVALRAGSDVDELTP"
     gene            1699..>1978
                     /locus_tag="AC16CY_21495"
     CDS             1699..>1978
                     /locus_tag="AC16CY_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356440.1"
                     /translation="MNRRLSLAIACSIALLGSYVYAQFGDKPAELSTIKVRDDLFVVY
                     NDFVPGNTTVLVTNQGLILVDNKFEIDFDNLMAQIRKISNQPIRYVVNT"
BASE COUNT          347 a          680 c          602 g          349 t
ORIGIN      
        1 agcgggatca tcttgaactc cggcggcacc acacgcactc tgacgttgcc gcgcgcatcg
       61 cccgtgcgcg cccgcgtgtc gaagtccagc tcgtctgccg acacttccat gctgtaggtc
      121 ttcatgcgga cgttgccacg gtactggacg accgctccct gccgtgtctg gcggtccgca
      181 gtgatgtcgt actcccggtc acggccgaac ggcatcggag gagcctgacg caacacctcc
      241 ggaatgggtc cgacccggag cctgaaaggc tgcaggtcga cgcgcgatcc gctgcgcagt
      301 tcgacgccgc cgacggaagc gagttggaat cctggcaacg tcgcgcggat cgcgtacgtg
      361 tcggggggaa gtgacagcgt aaacgacccg ttggcgccgg tcacgaccga gcccgccact
      421 gtcccgtcgg gccgcacggc ggtcaccgtc gcaccgggag ccggcgaacc ggacacatcc
      481 tggaccgtcc cggcgatcgc gcccggcgcc ggttcctgac cgaacgtggc caacgaggtg
      541 agaagaacga ggaacggcac tgcagccttc atcgcgcacc tccgcgctct caatttgtct
      601 ctcgtcgacg atcatgcctc agcgggagca tcttgatctc cggctgcggc ccccggaatc
      661 tgatgtcgcc gcgcgccacc ctgagcctga acggccgcag gtcgactcgc gcttcgctgc
      721 gaagctcgat tccgctttcc ccagcgggac ggaatccctg cagcgtcgcc cggacggtgt
      781 acctaccgga aggaagagac aggatgaacg agccgttgga actggtcacc acggtgcctg
      841 ccaatgtctc gtcctgccga aaggcggtca ccgtcgcgcc cggtatcgcc aaatcggacg
      901 cgtccagcac ggttccggcg atcacgcccg gcgccggctc ctgaccgaag acggccaagg
      961 aggtgagaag aacgagaacc ggcattgctg ccttcagagc gcccctccag caccaacgtt
     1021 catggggtca attcatcgac gtctgagccc gcgcgcaacg ctacgtcgaa cgtgcggtcc
     1081 agccgcgcgg tagccccatc cggcccggtc acctgcagcg cgaacaccag cttcctggcc
     1141 ggacgatcga tgcgtcgccg ctcgaccagc acgagttcgt ggttctcgtg aacaatcgcc
     1201 gtctcgtaca catcgtcctc gccgtcccgg tacccgaacg aatgcctgct ggaggtacca
     1261 cgaggcccgc gatgaccccc gccgccacca agaactgcaa aacactggtc ggggtttcgt
     1321 tcgcgcatct cactcatccg cgcttggatc cgtgcctggt ggcgttcgga catgtcgcgc
     1381 aaccattcac gcgcagcctc gcggccggtt ggaacgggcg gcgccggcgg agtccacgcc
     1441 tgcaggcgtt gcagcgccgc gtgtgcttgc ggaagcgcac catccggcag ggtcatcacg
     1501 aactgctgga gctcttctcg agaggtcatg gtccgattat gcctccgtgg aacacgcgtc
     1561 agccccgttg ggagccgacc ggggtttcgt tcgtcgcctt cgtgtcttcg tggccaaggc
     1621 cttcgtggcc ttcgtggttt tagagcgcga ccggtcgcga tagactctct acgcccatct
     1681 gaccggagga gccgccacat gaaccgccgt ctgagcctcg cgatcgcctg ttccattgcc
     1741 ctgctcggtt cctacgtcta cgcgcagttt ggcgacaagc cggcggaact gtcgacgatc
     1801 aaggtccggg acgatctgtt cgtcgtctac aacgatttcg tgcctggcaa taccacggtg
     1861 ctcgtcacca atcaggggct gatcctcgtc gacaacaagt tcgagatcga cttcgacaat
     1921 ctcatggcgc agatcaggaa gatctcgaat cagccgatcc gctacgtcgt caacacgc
//
LOCUS       DBNGSH010000940        12068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3859697, whole genome shotgun sequence.
ACCESSION   DBNGSH010000940 DBNGSH010000000
VERSION     DBNGSH010000940.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12068
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3859697"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..717)
                     /locus_tag="AC16CY_21500"
     CDS             complement(133..717)
                     /locus_tag="AC16CY_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIR3356441.1"
                     /translation="MTRGRIEAFSDGVIAIIITIMVLELPEPEGPEWSALRPVAPVFL
                     TYVLSFAYIGIYWSNHHHLLHSAQRINGRILWANLHLLFWLSLVPFVTGWMGHNHSAP
                     EPTAVYGVVMLLAAVAYWILQKTILLQHGSDSVLARAIGRDLKGKLSPVFYAAAIPLA
                     FVNQWLSDALYVAVALMWLVPDRRIELTLEREGK"
     gene            750..1556
                     /locus_tag="AC16CY_21505"
     CDS             750..1556
                     /locus_tag="AC16CY_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3356442.1"
                     /translation="MEPVLELQGATLVRGRARVLDDLTLTIQHGEHSVILGPNGAGKT
                     SLIRMLTLDDRPLASANGAPPMRIFGREKWDLVELRTRLGVVTGELDAGFGLGTSGGR
                     VSGLDTAMSGLFGSHGVFSHHEVTADARERALAALERVEASHLAARPLNEMSAGERRR
                     VLIARALVTRPDALVLDEPTTGLDFVARYRFMETIRRLAAEGTTIVLVTHHVDEVIPE
                     IAKVVLLRKGRVAFVGRPAEALTDERLSEVFGAPMTVERSGAYYYVKVAR"
     gene            1560..3026
                     /locus_tag="AC16CY_21510"
     CDS             1560..3026
                     /locus_tag="AC16CY_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3356443.1"
                     /translation="MAADRFPAFLSRYPEYQATQRLDELRAAEYGRLDEAGHTYLDYT
                     GASLHAQSQVRRHADLLSAHVLGNPHSASPASFASTELVERARQAVLDWFGAGVGYTA
                     IFTANATGALKHVGESYPFAPGGRLLLTVDNHNSVNGIREYARIRGASVSYAPLTTPE
                     LRIDQSRLGALLGEADPQARNLFAFPAQSNFSGVKHPLDLVARAQSAGWHVLLDAAAF
                     VPTDRLDLQAVQPDFVSVSFYKMFGYPTGVGCLLARKEALAVLERPWFSGGTVNFATV
                     GVRRHLLTPGEAGFEDGTLNYQSIAAVEVGLQHLASVGLDAIRTRVRCLTGWLLEQLI
                     SLRHSNGRPMVRIYGPLTTSMRGGIVTMNFYDPAGHLLDYRRVEELAAERSISFRTGC
                     FCNPGADETAEGITAEDLTAVLAERPDLTLQLFLQIVTARGGKSAGAIRVSFGLASNF
                     ADAERFLQFAETLRDQTRLAVGEVTFDIEAGRALRDGS"
     gene            3040..5016
                     /locus_tag="AC16CY_21515"
     CDS             3040..5016
                     /locus_tag="AC16CY_21515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3356444.1"
                     /translation="MQKPFGTWPSPITAQMVAAHGLRLSHVSVDGGDVYWVEGRPAEE
                     GRNVLVRRGADGRVADVTPPGFNVRTRVHEYGGGSCLVSGGVAYVSNFADQRLYKVDL
                     SKSRERTPAAGQPVPITPPGQWFYADAAFDAARARLIAVREDHSDPNPINTLVSVPVD
                     GPPHAGDVIASGHDFYSTPRLSPDGTKLAWLSWWHPQMPWDGTELWVADVTGSGRLAD
                     VERVAGGPSESIYQPGWTPDGTLVFVSDRDGWWRIRRWNHSTVADIVTNPPPDAEFGR
                     PQWVLGGATWAPAGPGRLVVSYTRAGRWYLAVVDLGRGSLSPLAGGLEPREWLTATAT
                     DVVLVAGSTTAPDAVVRVSIETGLVRMLRPSSAIDLNAAWISKPEPIEFPSGDGHVIR
                     AFYYAPRHPTCRAPDGERPPLIVIGHGGPTTATTAIFDARVQFWTSRGFAVVDVNYGG
                     STGFGRAYRERLKGQWGIVDVNDMVAAARYLVAQGKADPDRLAIRGGSAGGYTTLAAL
                     TFRAGVFKAGASYYGISDVEVLARDTHKFESRYLDGLIGPYPAARDVYRSRSPINFID
                     RLSSALILFQGLEDKVVPPNQSEMMAAAVRSRGLPVAYLAFEGEQHGFRRAATIARCL
                     EAELFFYGAVFGFTPADPIEPPAIDNLPRRTVRT"
     gene            5231..6217
                     /gene="nadE"
                     /locus_tag="AC16CY_21520"
     CDS             5231..6217
                     /gene="nadE"
                     /locus_tag="AC16CY_21520"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR3356445.1"
                     /translation="MTTIDCTWLSFDAAAEVERIVARLREILSRDLKRRGLVVGLSGG
                     IDSSVTAALGVRAFGADRVFGLLMPEKQSDPDTLAISQIVAEAFGLEFAHEDISVILE
                     SVGFYRRYDDAVRELVPQFADDWKSKVVIQNGGDVAGFNYFELVVQSPSGDILRQRLP
                     LKTYLGIVASTNFKQRVRKMLEYYHADRLNFAVAGTPNRLEYDQGFFVKNGDGSADVK
                     PIAHLYKSQVYELGAYLKVPEKVRNRLPTTDTYSLAQAQDEFYFLVPYHQMDLCLYAK
                     NNGISLEETVRVSGLSVDKVQRVWIDIDRKRAATRYHHLQAVLVADVPEIAL"
     gene            6249..6500
                     /locus_tag="AC16CY_21525"
     CDS             6249..6500
                     /locus_tag="AC16CY_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3356446.1"
                     /translation="MSDTRTRVRGFIVEHFLFGQGATLRDDSSFLEQGIIDSTGVLEL
                     IAFLEQTFSIKVSDHELVPDNLDSVDRVCVYLASKTPKT"
     gene            6537..8063
                     /locus_tag="AC16CY_21530"
     CDS             6537..8063
                     /locus_tag="AC16CY_21530"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3356447.1"
                     /translation="MLVHELLTRSAHWRPEQVALVCGGRRFSFRELDEMSNQVAQALV
                     QRGVDRGDRVALYLPNCPEAVLGVFGVLKAGAVFVMVHPTTKREKLHYILHDCGASAV
                     LVDEALPARFDESAFPDLKAVLSRERAGAPVRASGSRACSFGAVLADCSKDAPPLRNI
                     DLDLACLVYTSGSTGEPRGVMCDHRNVVFVTQSIVEYLGNNESDIVLSVLPLSFSYGL
                     YQLMAVMCSGARLVLEDSFAFPAAILHTMAAERVTGFAGVPTMYAVLLGLDLGAFDLS
                     ALRYLTNAAAALPVDHVRRLREQVPHAALFLMHGLTEVARSVYLPPDQVDARPSSVGR
                     AIPGTEVWLEDEHGRRVGAGEIGELVVRGRNVMRGYWNNAEATQARFRPGPTPGERLC
                     YSGDLFRADEEGFLYFVSRQDDIIKSRGEKVSPREVEEVLYAIEGVTEAAVIGVPDPV
                     LGQAIKAFVVAPGRGLASPQVLAHCKAHLEDVLVPKFVEFRDELPKTPTGKIRKTDLR
                     "
     gene            8063..10042
                     /gene="asnB"
                     /locus_tag="AC16CY_21535"
     CDS             8063..10042
                     /gene="asnB"
                     /locus_tag="AC16CY_21535"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3356448.1"
                     /translation="MCGIAGIVDAQSVPADREGLVRRMLGAIRHRGPDQFGLFVDRRA
                     ALGHARLSIIDLIGGQQPIANEDDTLWIVFSGEIFNYVELRAELETRGHRFATRTDTE
                     VIVHLYEELGPDCLSRFNGQFAIAIWDTRRQALLLARDRLGVRPLFYTRVGQALLFAS
                     EIKSLFCDARVRREIDPISLDQTFTYWAPLSPRSAFRDVFEIPPGHYLLAQGGDQAIK
                     PYWTLDFHPAADSGGESTSALVDEFRSLLVDAVRIRLRADVPVGAYVSGGLDSSTIAS
                     VVRRFTSNRLSTFSIAFDDANFDESAFQQRMAGELGTEHQVVHATHAQIGQAFPDVVW
                     HAEVPLMRTAPAPMFLLSQLVRRSGYKVVLTGEGADEFLCGYDIFKEDKVRRFWARQP
                     DSRWRWLLLQRLYQDIARLSESTGAFLAAFFKDRLTEVECPWYSHLIRWRNSRRSCRF
                     FSAGMAAGSVGGPDEYLRSVPLPRRFGEWSPTERAQYWEITTFLSPYLLSSQGDRMAM
                     AHSVEGRHPFLDHRMVELASRLPSRMKLRVLRDKHILRAASRDWLPAEIRQRPKRPYR
                     APIHRSFFGGPTLEYVDTLLSERMVAHVGVFEPKAVTQLVTRSRAGAALGETDEMALA
                     GILSTQLLHHQFVDSFPDVPPVGGNDDVKVCLATP"
     gene            complement(10039..10794)
                     /locus_tag="AC16CY_21540"
     CDS             complement(10039..10794)
                     /locus_tag="AC16CY_21540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3356449.1"
                     /translation="MPQVFDRRVRALRAAWKRSGMEGVAMLALGSQLVPRQLLFAHDV
                     DVLKLVELNVPAFVASTGAYEFSTADASILEELVECTDVPVSIDRRRKRLAQALALCA
                     SCITVRHQGRLVAYACAFVGKYVLTYDDYGPRTLTFALDQHAVFLGNGFIRPEYRMKG
                     LFPHLLRNCVERYPTGTRFFGHIDTDNVQSFNSHRRLGFAPLLTITCLGIGPARFFFQ
                     RPFGTRRRIRVEHHAALRLVEHDGGLALGTTRH"
     gene            complement(10835..>12068)
                     /locus_tag="AC16CY_21545"
     CDS             complement(10835..>12068)
                     /locus_tag="AC16CY_21545"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3356450.1"
                     /translation="RRYAIIGTGDRATSMWGRPVLQEYSDVVELVGLCDVNPIRAEAS
                     KQLMGASCPVFTRFDEMCERAKPDLLMVTTVDSFHHEYIVKALDRGIDVITEKPMVID
                     EKQCQAVLDAEARNRRKIIVTFNYRYAPRHRIVKELLMSGEIGRVLSVDFAWYLDVIH
                     GADYFRRWHRLRNGGGTLFVHKATHHFDLVNWWLDADPVEVVASGDLKVYGRNSPFRH
                     THCRPCPHKTECRFHYDMTNDATRMKLYASAEGVDGYHRDGCVFREDVNIFDTMSAIV
                     KYSNGVTMSYELDAHLPYEGFAIAFNGEFGRIDVRDHERQPWKVAEEDETEIYLTKSF
                     GVRRRIPITGSTVGGHGGGDTRLRDLIFRKADAPDHLKLPDSRAGAMSCLTGIAARKS
                     ADEKRAVRLADLVRFPNA"
BASE COUNT         1945 a         3921 c         4035 g         2167 t
ORIGIN      
        1 ctcggctcgg tcagcgcgga acatcgcatc ttcggatcgc tcacgatgta ccagtgggtc
       61 gagctgacgg ccgcccacga gatgcggcac gcggatcaga ttcgggaaat cgcaggcgcg
      121 ctcggccgct agctattttc cttccctctc gagcgtcagc tcaattcgcc gatcgggcac
      181 cagccacatc aatgcgactg ccacatacag cgcgtcggag agccactggt tgacgaatgc
      241 cagcgggatg gcggctgcgt agaacaccgg cgagagcttg cccttcaggt cgcggccgat
      301 cgccctggcg agcaccgagt cggatccgtg ttgcaggagg atcgtcttct gcaggatcca
      361 gtaggccacc gcggccagaa gcatcacgac gccgtagacg gccgtgggtt cgggggcgga
      421 gtgattgtga cccatccagc cggtgacaaa cggcacgagc gacagccaga acagcaggtg
      481 caggttcgcc cacagaatcc ggccgttgat gcgctgcgcg gagtgcagca ggtggtggtg
      541 attgctccag tagataccga tgtacgcgaa gctgaggacg taggtgagga acacgggagc
      601 caccggacgg agcgcggacc actccgggcc ttccggttcc ggcaactcga ggaccatgat
      661 cgtgatgatg atcgcgatca ctccgtcgct gaaggcttcg attcgtcctc gtgtcatcgt
      721 cgatgttgta ttcgagagag aatagtagcg tggaaccggt actggagctg cagggcgcga
      781 cgctcgtgag agggcgcgcg cgcgtgctcg acgacctgac gctgacgatc cagcacgggg
      841 aacactccgt catcctcggc ccgaacggcg cgggcaagac ctcgctcatt cgcatgctga
      901 cgctcgacga tcgtccgctg gcgtcggcga acggggcgcc gcccatgcgc atcttcggcc
      961 gcgagaagtg ggacctcgtc gagctgcgca cgcggctggg cgtggtcacc ggcgagctgg
     1021 acgccggctt cggcctcggg acctctggtg gccgcgtctc cgggctcgat acggcgatgt
     1081 cggggttgtt cggcagccac ggcgtcttct cgcaccacga ggtgacggcc gacgcgcgcg
     1141 aacgcgcgct ggcggcactc gagcgggtcg aggcctcaca cctggctgcc aggccgctga
     1201 acgagatgtc ggccggtgaa cggcggcggg tgctgatcgc ccgggcgctg gtcacgcggc
     1261 ccgacgcgct cgtcctggac gagccgacga ccgggctcga tttcgtcgcc cgctaccgct
     1321 tcatggagac catccggcgc ctggccgccg agggaacgac gatcgtgctc gtgacgcatc
     1381 acgtcgacga ggtcatcccg gaaatcgcga aggtcgtcct tctgcgcaag gggcgcgtcg
     1441 ccttcgtcgg ccgtcccgca gaggcgctga ccgacgagcg gctgagcgag gtgttcggcg
     1501 ctcccatgac cgtcgagcgg agcggcgcgt actactacgt gaaggtggca cgctgacccg
     1561 tggccgccga tcgattcccg gcgttcctga gccgctaccc tgagtaccag gcgacgcagc
     1621 gcctcgacga gctgcgcgcc gccgagtacg ggaggctcga cgaggcgggc catacctatc
     1681 tcgattacac cggcgcgagc cttcacgccc agtcgcaggt ccgacggcac gcggacctgc
     1741 tcagcgcgca cgtgctcggg aacccgcact cggcaagtcc ggcgtcgttt gcctcgacgg
     1801 agctcgtcga gcgtgcgcgt caggccgtgc tcgactggtt cggcgccggc gtcggctaca
     1861 ccgcgatctt cacggccaac gcgacgggcg cgctcaagca cgtcggcgag tcgtacccct
     1921 tcgcgcccgg cggccgcctc ctgctgacgg ttgacaatca caactcggtg aacggcatcc
     1981 gcgagtacgc gcgtatccgc ggcgcctcgg tctcgtatgc gccgctcacg accccggaac
     2041 tgcgaatcga tcagtccagg ctcggggcgc tgctcggcga ggcggatccg caggcccgca
     2101 acctgttcgc gtttccggcg cagtcgaact tctccggtgt gaagcatccg ctcgatctcg
     2161 tcgcgcgggc gcagtcggcg ggctggcacg tgctgctcga cgcggccgcg ttcgttccta
     2221 ccgatcggct ggacctgcag gccgtccagc cggacttcgt gagcgtgtcc ttctacaaga
     2281 tgttcggcta cccgaccggc gtcggctgtc tgctcgcacg caaagaggcg ctggccgtgc
     2341 tcgagcgccc ctggttctcc ggtggcaccg tcaacttcgc gaccgtgggc gtgcggcgcc
     2401 atctcctgac gcccggtgaa gcgggcttcg aggacgggac gctcaattac cagtccattg
     2461 ccgccgtcga ggtcgggctg cagcacctcg cgtcggttgg cctcgacgcc atcaggacgc
     2521 gagtgcggtg tctgacaggg tggctgctcg agcagctcat cagccttcgg cattcgaacg
     2581 gacgaccgat ggtgcgcatc tacggcccgc tcacgacgtc gatgcgcggc gggatcgtca
     2641 cgatgaactt ctacgacccc gccggccacc tgctggacta ccgacgcgtc gaggagctag
     2701 cggccgaacg gagcatttcg ttcagaactg gctgcttttg caatcccggt gccgacgaga
     2761 cggccgaggg gatcacggcg gaagacctca ccgccgtcct ggccgagcgt cccgacctga
     2821 cgctgcagct ctttcttcag atcgtgacgg cccgcggggg caagagcgcc ggggccatcc
     2881 gcgtgtcgtt cggcctcgcg agcaacttcg ccgacgccga gcgcttcctc cagtttgcgg
     2941 agacgctccg cgatcagacg cggctcgccg tcggcgaggt gacattcgac atcgaggcgg
     3001 gccgggccct cagggacgga agctgagacc ctgaaggcca tgcagaagcc gtttgggacc
     3061 tggccgtctc cgattaccgc gcagatggtc gcggcacacg gccttcgcct gagccacgtc
     3121 agcgtggacg gcggcgacgt gtactgggtc gaggggcgcc ccgcagaaga ggggcgcaac
     3181 gtgctcgtcc gccgcggagc ggacggacgc gtggccgacg tcacgccacc cggcttcaac
     3241 gtgcggacgc gcgtccacga gtacggcggc ggcagttgcc tcgtctcggg tggcgttgcc
     3301 tacgtgtcga acttcgccga tcagcgtctg tacaaggtcg acctctccaa gtctcgagaa
     3361 cgcacgccgg cagcgggcca gccggttccg atcacgccgc cggggcaatg gttctacgcg
     3421 gacgccgcat tcgacgccgc gcgcgcgcgg ctgatcgccg tcagagaaga ccactccgat
     3481 ccgaacccga tcaacacgct ggtgagcgtg cctgtggacg gcccgccgca tgcgggagat
     3541 gtgatcgcgt ccgggcacga cttctattcg accccgaggc tgagtcccga cgggacaaag
     3601 ctcgcgtggc tttcgtggtg gcacccacag atgccctggg atgggaccga gctgtgggtc
     3661 gccgacgtga ccggatcggg caggctcgcc gacgtcgagc gcgtggctgg cgggccatcg
     3721 gaatcgatct accagcctgg ctggacgccc gacggaacgc tcgtcttcgt gagcgatcgc
     3781 gatggctggt ggaggatccg tcgctggaac cattcgacgg tcgctgacat cgtcaccaat
     3841 ccgcctcccg acgccgagtt cggacggcct caatgggtac tgggaggcgc cacctgggcg
     3901 ccggcgggcc ctgggcgcct ggtcgtgtcg tacacccgag cgggccggtg gtacctggcg
     3961 gttgtggatc tcggacgcgg ctcattgtca ccgctggctg ggggactcga gccgcgggag
     4021 tggctgacgg cgaccgccac cgacgtcgtg ctcgttgccg gatccacgac agctcccgac
     4081 gccgtcgttc gcgtgtcgat cgagaccggc ctggtccgga tgcttcggcc ctcgtcggcg
     4141 atcgatctga atgctgcctg gatctcgaag cccgagccaa tcgaattccc ttccggggat
     4201 gggcacgtga ttcgcgcctt ctactacgcg ccgcgacacc cgacctgcag ggcgcccgac
     4261 ggggaaagac caccactcat cgtgatcggg cacggcggtc cgacgacggc caccacggcg
     4321 atcttcgatg cccgggtaca gttctggacc agtcgggggt tcgccgtcgt tgacgtgaac
     4381 tacggcggca gcaccgggtt tggacgcgcc taccgtgagc gtctcaaggg ccagtggggc
     4441 atcgtggacg tgaacgacat ggtggccgcc gcgaggtatc tcgtggcgca gggcaaggcc
     4501 gaccccgatc gcctcgccat tcggggggga agcgcgggag gctacacgac gcttgcggcg
     4561 ctgacgtttc gtgccggcgt gttcaaggcg ggtgcgagtt actacgggat cagtgacgtc
     4621 gaggtgctcg cgcgcgacac ccacaagttc gagtcgcgct acctggacgg cctcatcggc
     4681 ccgtatcccg cagcgcggga cgtgtaccgc tcgcgttcgc caatcaattt catcgatcgg
     4741 ctgtcgagtg cactgattct cttccagggc ctggaggaca aggtggttcc gccgaaccag
     4801 tcggagatga tggcagcggc ggtcaggtcc agggggcttc cagttgcgta tctcgccttc
     4861 gagggggagc agcatggttt ccgccgggcc gcgacaatcg cgcgatgcct cgaggcggag
     4921 ctgttcttct acggtgccgt gttcggcttc acgccggccg atccgatcga gccgcccgcg
     4981 atcgacaacc ttccgcgtcg caccgtccgg acttgaaacg ctgtggagga gcgccagttc
     5041 cccgaatcgg aggaccggcg agccgccgga ccatccggcc gtacgcactc gcccacggcc
     5101 ccttctgtgg tccgccccgg cactcgtcgc ggggactttc aagtggttcg ggacgtgaat
     5161 tggggcggac ctgcggggcg gtcgcgccgc ggttcgcgtg gctcaccggg ggtgaaataa
     5221 tcagtcagtc atgaccacca tcgactgcac gtggctctcg ttcgatgccg ccgcggaagt
     5281 ggagcggatt gtggcccgtc tccgggagat cctctcccgg gacctcaagc gtcgcggtct
     5341 ggttgtcggg ctgtcgggcg gcatcgacag ttcggtgact gcagcgctgg gggttcgggc
     5401 ctttggcgcc gatcgggtct tcggcctgtt gatgcccgag aagcaatcgg acccggacac
     5461 gcttgcgatc agccagatcg tggcggaagc cttcggcctc gagttcgcac acgaggacat
     5521 cagcgtcatt ctggaatcgg tcggattcta tcggcgctac gacgacgccg tgcgcgagtt
     5581 ggtaccccag ttcgctgatg actggaaatc gaaggtggtc atccagaacg gcggtgatgt
     5641 cgccgggttc aattacttcg aactcgtggt gcaatccccg tcgggtgaca tcctccgcca
     5701 gcggcttccg ctcaagacgt acctcggcat cgtcgcctcc acgaatttca agcagcgcgt
     5761 tcgcaaaatg ctggagtact atcacgccga tcgactgaac ttcgccgtgg ccggcacgcc
     5821 gaaccgtctc gagtacgacc agggcttctt cgtgaagaac ggcgacggct ccgccgacgt
     5881 gaaacccatc gcgcatcttt acaagagtca ggtatacgaa ctgggcgcct atctgaaggt
     5941 gcccgagaag gtccggaacc ggctgcccac gaccgacacc tactcgctgg cgcaggcgca
     6001 ggatgagttc tatttcctcg tgccgtacca ccagatggat ctgtgtctgt acgcgaagaa
     6061 caacggcatc agcctcgagg agaccgtgag ggtctccggc ctgtccgtgg acaaggtcca
     6121 gcgcgtctgg atcgatatcg accgcaagcg ggcagccacc cgctatcatc atttgcaggc
     6181 ggtgctggtg gccgatgtcc ccgagatcgc actctaggcc cagccggcgc accgatgcct
     6241 gaatctgtat gagcgacact cggacacgcg tccgcggatt catcgttgaa cacttcctct
     6301 ttgggcaggg cgcgacgctg cgtgacgaca gctccttcct cgagcagggc atcatcgatt
     6361 cgaccggcgt gctggagctg atcgcgttcc tggaacagac cttttccatc aaggtgtccg
     6421 accacgagct ggtgccagac aacctggact ccgtcgatcg cgtctgtgtg tatctcgcca
     6481 gcaagacgcc gaagacctag caggatctgt cccccgcctc tgtcaagggt tccttcatgc
     6541 tggttcacga gttgctcacc cgaagcgccc actggcgtcc ggagcaagtc gcgctcgtct
     6601 gtggcggacg gcgtttttca tttcgagaac tggacgagat gtccaaccag gtggcccagg
     6661 cgctcgtcca gcgaggagtc gaccgcggcg accgcgtggc cctctatctg ccgaactgtc
     6721 ccgaggctgt cctcggcgtc ttcggggtgc tcaaggccgg cgccgtgttc gtcatggtcc
     6781 acccgacgac gaagcgcgag aagctccact acatccttca tgactgcggg gcgtcggccg
     6841 tgctggtaga cgaggccctg cccgcgcggt tcgacgagtc ggcctttcca gatctgaagg
     6901 ctgtgctcag ccgcgagcgg gctggggcgc cggtcagggc ctcaggctcg cgtgcgtgtt
     6961 cgttcggagc ggtgctggcc gactgttcga aggatgctcc acctctccgc aacatcgatc
     7021 tcgatctggc ctgcctcgtc tacacctccg gtagcacggg cgaaccgagg ggcgtgatgt
     7081 gcgatcaccg caacgtggtg tttgtcacgc aatcgatcgt cgagtacctg ggcaacaacg
     7141 agtccgatat cgtgctcagc gtcctgccgc tgtcattcag ctacgggctg taccaattga
     7201 tggcggtgat gtgctctggt gcgcggctgg tcctggagga ctccttcgcc ttcccggcgg
     7261 ccattctgca cacgatggcg gccgagcgag taaccggctt cgccggcgtg ccgacgatgt
     7321 acgccgtgct tcttggactc gatctcggcg ccttcgattt gtcggccctg cgctatctga
     7381 cgaatgccgc cgccgcgctg ccggtcgacc acgtgcggcg gttgcgggaa caggttcccc
     7441 acgccgcgct cttcctgatg catggcctga ccgaagtcgc tcgcagcgtg tatctgccgc
     7501 ccgatcaggt cgacgcccgc ccttcgtcag tgggtcgcgc catacccggc accgaggtct
     7561 ggctcgagga tgagcacggc cggcgagtgg gcgctgggga gattggcgaa ctggtcgtgc
     7621 ggggccggaa tgtcatgcgc ggctactgga acaatgccga ggcgacacag gcgcgctttc
     7681 gtcccgggcc tacccccggc gagcgcctct gctacagcgg ggacctgttc cgggcagacg
     7741 aggagggctt cctctatttc gtctcgcgcc aggacgacat catcaagagt cgcggcgaga
     7801 aagtgtcgcc ccgtgaagtc gaagaagtgc tctatgccat cgagggcgtg acggaagccg
     7861 ccgtcattgg cgtgccggac ccggtgttgg gccaggcgat caaggcgttt gtggtcgcgc
     7921 cgggccgggg cctggcatcg ccgcaggtgc tggcgcactg caaggcgcac ctggaggatg
     7981 tgctggtgcc gaagtttgtc gagtttcgag acgaacttcc gaagaccccg accggcaaaa
     8041 tccggaaaac ggacttgcga taatgtgcgg tatcgcgggg atcgtcgacg cgcagtcagt
     8101 tccggcggac cgtgagggac tcgtccggcg gatgctcggc gcgatccgcc atcgtggtcc
     8161 ggatcagttc ggcctgttcg tggaccgtcg cgctgccctc ggtcacgccc gcctcagcat
     8221 catcgacctg atcggagggc agcagcccat tgcgaacgaa gacgacacgc tctggattgt
     8281 gttcagcgga gagatcttca actatgtcga gctccgcgcg gagctggaga cacgcggtca
     8341 tcgcttcgcg acgcgtacgg atacggaggt catcgttcac ctctacgagg agctcgggcc
     8401 ggattgcttg agccggttca atggccagtt tgccatcgcc atctgggaca cccgccgcca
     8461 ggcgctcctg ctcgcccgtg atcgcctggg tgtgcgtccg ctcttctaca cccgggtggg
     8521 ccaggcgctg ctcttcgcgt ccgaaatcaa gtcgctgttc tgcgatgcgc gcgtgcggcg
     8581 tgagatcgat ccgatcagtc tcgatcagac cttcacgtac tgggcgccgc tctcgccgcg
     8641 ctccgcgttt cgcgacgtct tcgagatccc accggggcac tatctgctgg cgcagggcgg
     8701 cgatcaggcg atcaagcctt actggaccct ggactttcat ccggcggctg actcgggcgg
     8761 ggaatccacc tcggcgctcg tcgacgagtt tcgctcgttg ctcgttgacg ccgtccgcat
     8821 ccgcctccgc gcggacgtgc cggtcggcgc gtatgtgagc ggcgggctgg attcatcgac
     8881 gatcgcgtcg gtcgtccgtc gtttcacctc caatcgcctg tccacgttct cgatcgcctt
     8941 tgatgacgcc aacttcgacg agagcgcgtt tcagcagcgg atggccgggg agctcggcac
     9001 cgagcaccag gtggtgcacg caacgcacgc ccagatcggt caggcatttc ccgacgtcgt
     9061 ctggcatgcc gaggtaccgc tgatgcggac cgcacccgcg ccgatgtttc tgctgtcgca
     9121 actcgtccgg cgttccggct acaaggtcgt gttgacgggt gaaggggcgg acgaattcct
     9181 gtgtggttac gacattttca aagaagacaa ggtccggcgg ttctgggcgc ggcagccgga
     9241 ttcgcgctgg cgctggctgt tgttgcagcg cctgtaccag gacatcgcgc ggctgtcgga
     9301 gagcactggc gcattcctcg ccgcgttctt caaggatcgc ctcactgaag tcgagtgccc
     9361 gtggtactcg catctgatcc gctggcgcaa cagccgccgg tcgtgtcgat tcttctctgc
     9421 tggcatggcc gccggatcgg ttggcgggcc ggatgagtac ctccggtctg tcccattgcc
     9481 gaggcggttt ggcgagtgga gcccgaccga gcgggcgcag tactgggaga ttacgacgtt
     9541 cctctcgccg tacctcctct cgtcgcaggg cgaccggatg gccatggccc actccgtcga
     9601 aggccgccat ccgttcctcg accatcgcat ggtggaactg gcgagtcgcc tgccgtcccg
     9661 gatgaagctg cgggtgctgc gcgacaagca catcctgcgc gcggccagcc gcgactggtt
     9721 gcccgccgag attcgccagc gcccgaaacg cccctatcgc gctccgatcc accgcagttt
     9781 ctttggcggg ccaacgttgg agtacgtcga tacgctgctg tcggagcgta tggtcgcgca
     9841 cgtcggcgtc tttgaaccga aggcggtgac ccagctcgtg acgcggagcc gggccggcgc
     9901 cgctctcggc gaaacggacg agatggcgct cgccggcatt ctctccactc aactcctgca
     9961 ccatcaattc gtggacagtt tccctgacgt tccacccgtg ggcgggaacg acgatgtgaa
    10021 ggtgtgcctc gcgactcctt aatggcgcgt tgtcccgagc gccaggccgc cgtcgtgctc
    10081 gacgagccgg agcgctgcat gatgctcgac gcgaatgcgc cgccgcgtgc cgaacggacg
    10141 ctggaagaaa aagcgcgcgg ggccaatacc gagacacgtg atggtgagaa ggggggcaaa
    10201 tccaagacgg cgatgggagt tgaacgactg cacgttgtcg gtgtcgatgt gcccgaagaa
    10261 ccgggtgccc gtggggtagc gctccacgca gttgcgcagg aggtgcggga agagaccctt
    10321 catccggtac tcgggacgga tgaagccgtt gccgaggaaa acggcgtgct ggtcgagcgc
    10381 gaaggtgagc gtccgtggcc cgtagtcgtc gtacgtcagc acgtacttgc ccacgaacgc
    10441 gcaggcgtag gccaccagac ggccctggtg gcgcaccgtg atgcaggagg cgcagagggc
    10501 cagggcctgc gccaacctct tgcggcgacg atcgatggag accggaacgt ccgtgcactc
    10561 cacgagctcc tccaggatcg acgcgtcggc cgtcgagaac tcgtacgcgc cggtggacgc
    10621 gacgaatgcg gggacgttca gctcgacgag cttgagcacg tcgacgtcgt gcgcgaacaa
    10681 gagctgtcgc ggtacgagct gggacccgag ggcgagcatc gcgacgccct ccatgccaga
    10741 ccgtttccac gcggcgcgca aggccctgac tcggcgatcg aacacctgcg gcacgtcagt
    10801 attctgcctc tccgcccgac gacatcgcgc ccgcctatgc gttcgggaat cggaccagat
    10861 cggcgagccg cacggcgcgc ttctcgtccg cgctcttgcg cgccgcgatg cccgtgaggc
    10921 acgacatggc gcccgcgcgc gagtcgggga gcttcaagtg gtcgggcgcg tccgctttcc
    10981 ggaagatcag gtcgcgcaac cgcgtgtcgc cgcctccgtg cccgccgacg gtgctgccgg
    11041 taatcggaat gcgcctccgg acgccgaagc tcttcgtgag gtagatctcc gtttcgtcct
    11101 cctcggccac cttccacggc tgccgttcgt ggtcgcgcac gtcgatacgc ccgaactcgc
    11161 cgttgaacgc gatcgcgaac ccctcgtacg gcaggtgggc atcgagctcg tagctcatgg
    11221 tgacgccgtt cgagtacttc acgatcgccg acatggtatc gaagatgttc acgtcctccc
    11281 ggaagacgca gccgtcgcga tggtagccgt ccacgccctc cgcggatgca tagagcttca
    11341 tccgcgtcgc atcgttggtc atgtcgtagt ggaaccggca ctctgtcttg tgcgggcacg
    11401 ggcggcaatg ggtgtggcgg aatgggctgt tccgcccgta caccttcagg tccccgctcg
    11461 ccacgacctc cacgggatcg gcgtcgagcc accagttgac gaggtcgaag tggtgcgtcg
    11521 ccttgtgcac gaacagcgtg ccgccgccgt tgcgaagccg gtgccaccgg cggaagtagt
    11581 cggcgccgtg gatcacgtcg aggtaccagg cgaaatccac cgagagcacg cggccgatct
    11641 cccccgacat cagcagctcc ttgacgatgc gatggcgcgg cgcgtagcgg tagttgaacg
    11701 tgacgatgat cttccgcctg ttgcgtgcct cggcgtcgag gacggcctgg cactgcttct
    11761 cgtcgatgac catcggcttc tcggtgatga cgtcgatgcc gcggtcgagc gccttcacga
    11821 tgtactcgtg atggaaactg tcgaccgtgg tcaccatcag caggtccggc ttcgcccgct
    11881 cgcacatctc gtcgaagcgg gtgaagacgg ggcaggacgc gcccatgagc tgcttcgacg
    11941 cctcggcgcg tatcggattg acatcgcaca atccgacaag ctcgacgacg tcggaatact
    12001 cctgaagaac cgggcgtccc cacatggacg tcgcgcgatc gccggtgccg atgatggcgt
    12061 atcgccgg
//
LOCUS       DBNGSH010000941         2420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2333680, whole genome shotgun sequence.
ACCESSION   DBNGSH010000941 DBNGSH010000000
VERSION     DBNGSH010000941.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2333680"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC16CY_21550"
     CDS             <1..189
                     /locus_tag="AC16CY_21550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356451.1"
                     /translation="LKVTVTGLELDPTTDRVIVGRQATAPEAPAFRVDDPRNAAPETK
                     CRCSNAGASAAAALGSTP"
     gene            186..935
                     /locus_tag="AC16CY_21555"
     CDS             186..935
                     /locus_tag="AC16CY_21555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3356452.1"
                     /translation="MIAVRDLVKEYGRFRAVDGVTLDVKPGEIHGFLGPNGAGKTTTL
                     RMIAGLLKPTSGRILVNDHDLEREPEAAKAALGFIPDRPFIYDKLTAGEFLTFHAGLY
                     GFSNGSVQGRVHEMLDLFELRQWEHQLVESFSHGMKQRLVMSSAFFHRPRAVAVDEPM
                     VGLDPRGARLIKDVFRRMSERGVAILMSTHTLEVAQEMCHRVSIIQKGRIIAHGTVDE
                     VKQLAGGADEHLTSVFLKLTGGSGLQEIDEI"
     gene            937..>2420
                     /locus_tag="AC16CY_21560"
     CDS             937..>2420
                     /locus_tag="AC16CY_21560"
                     /inference="COORDINATES: protein motif:HMM:NF028259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit"
                     /protein_id="HIR3356453.1"
                     /translation="MTPSATAFPHLLLPHLWASRNRVRRGEKGDLLRGLVFGSIGLLV
                     SGALYYGAFWVTMQLAAYEEFGDYLLRLGLSWLFLTFLAFLAFSGIVTALSTFFLADD
                     LRLLMASPVAARRLFLARFARTVGSASWMVVIFMAPVLIGVGVGACAPPGFYATAAAT
                     VVPFCVIPVALGSACTLFVVNVFPARRARDLLMLMGLVFAAAVVLVLRFIQPERLLRV
                     DSLPDITDFFATLQSPVTPLLPSFWAGESLFASVHGGFDWLHQAALWTTAGAFTIVLG
                     AAHERWHFAGYSRSQEAPKTRFARFRTLDVLAASLPLSAVRRQLLVKDVKIFLRDVSQ
                     WVQLLPLLALVLLYLYNFRVLDLDRIPYMSGFVKNVYAFINLGMAGFVMATVAVRFVF
                     PAVSAEGQAFWIIRTSPISMHDFLWSKFWTALVPVLLFTEVLTIAANELLGVDPFLKI
                     VAAVAIVVLTFALVGLATGLGARYPRFGADATQAAGSYGGVMFMV"
BASE COUNT          329 a          817 c          804 g          470 t
ORIGIN      
        1 ttgaaggtga cggtgaccgg cctcgagctc gatccgacaa ccgaccgcgt gatcgtcggc
       61 cgtcaggcca ctgcgcccga ggctcccgcc tttcgcgtgg acgatcctcg caacgcggcg
      121 ccggagacga agtgccgctg ttcaaacgcc ggagcgtcgg cggcagccgc gctagggagc
      181 accccatgat tgcggtacgg gatctggtca aggagtacgg ccgcttcagg gcggttgacg
      241 gcgtcacgct cgacgtgaag ccgggggaga ttcacgggtt tctcggacca aacggcgccg
      301 gcaagacgac gacgctgcgg atgattgcgg ggctgctgaa gcccacgtcc ggccgcatcc
      361 tcgtcaacga tcacgatctc gagcgggagc ccgaggcggc gaaggcggcg ctcggcttca
      421 tccccgaccg gccgttcatc tacgacaagc tcacggccgg cgagttcctg acgttccacg
      481 ccgggcttta cggtttcagc aacggcagcg tgcaggggcg cgtgcacgag atgctcgacc
      541 tcttcgagct ccggcagtgg gagcaccagc tggtcgaaag cttctcgcac ggaatgaaac
      601 agcggctcgt catgagctcc gcgttctttc atcggcctcg cgccgtcgcc gtggacgagc
      661 cgatggtggg cctcgacccg cgcggcgcgc gcctcatcaa agacgtcttc cggcgcatga
      721 gcgagcgcgg cgtggcgatc ctgatgagca cccacacgct cgaagtggcg caggagatgt
      781 gccaccgcgt cagcatcatc cagaaggggc gcatcatcgc gcacggcacg gtagacgagg
      841 tcaagcagtt ggcgggtggc gcggacgagc atctgacgtc cgtcttcctg aagctcaccg
      901 gcggcagcgg cctgcaggag atcgacgaga tctgagatga cgccgtcggc gacagcattt
      961 ccgcatctgc tccttccaca cctctgggcg tcgaggaatc gcgtccgcag aggcgagaag
     1021 ggcgatctgc tcagggggct cgtcttcggt tcgatcggcc tgctcgtttc cggagcgctc
     1081 tactacggcg ccttctgggt gaccatgcag cttgccgcct acgaggagtt cggggactac
     1141 ctgcttcgcc tgggcctctc gtggctgttc ctcaccttcc tcgcctttct cgcgttcagc
     1201 ggcatcgtca cggcgctgtc cacgttcttc ctggccgacg accttcgtct cctgatggcg
     1261 agtcccgtgg ccgcgaggcg gctgttcctg gcccgcttcg cgcggacggt cgggtcggcg
     1321 tcgtggatgg tcgtgatctt catggcgcca gtgctcatcg gcgtgggcgt cggggcgtgc
     1381 gcgcctcccg gcttctacgc caccgcggcg gccacggtcg tgccgttctg cgtgataccg
     1441 gtcgcgctcg gctcggcgtg cacgctcttt gtcgtgaacg tgtttcccgc gcgccgcgcg
     1501 cgcgatctgc tgatgctgat ggggctcgtc ttcgcggcgg ccgtcgtgct ggtccttcga
     1561 ttcattcagc ccgagcgcct gctccgcgtc gattccctgc ccgacatcac cgacttcttc
     1621 gcgacgctgc agtcgccggt cacgccgctc ctgccgtcgt tctgggccgg agagtcgctc
     1681 tttgcctcgg tgcacggcgg gttcgactgg ctgcaccagg ccgcgctctg gacgacggcc
     1741 ggcgccttca ccatcgtgct cggcgcggcc cacgagcggt ggcacttcgc cgggtacagc
     1801 cgatcgcagg aagcgcccaa gacccgcttc gcgcgcttcc gcacgctcga cgttctcgcc
     1861 gcgtccctgc cgctgtcggc ggtccggcgt caactgctgg tgaaggacgt gaagatcttc
     1921 ctgcgcgacg tgagccagtg ggtgcagctg ctgccgctgc tggccctcgt cctgctgtat
     1981 ctctacaact tccgcgtgct cgatctcgat cgcattccct acatgagcgg cttcgtcaag
     2041 aacgtgtacg ccttcatcaa cctcgggatg gccgggttcg tgatggcgac cgtggcggtg
     2101 cgcttcgtct ttcccgccgt gtccgcggaa gggcaggcgt tctggatcat caggacctcg
     2161 cccatctcga tgcacgactt cctgtggtcg aaattctgga cagcgctcgt gcccgttctg
     2221 ctcttcacgg aggtgctgac gatcgcggcc aacgagctgc tgggcgtcga tccgttcctc
     2281 aagatcgtgg ccgcggtcgc gatcgtcgtc ttgaccttcg cgctggtcgg cctggccacg
     2341 gggctcggcg cgcgctaccc gcgcttcggc gccgacgcca cgcaggcggc gggctcgtac
     2401 gggggcgtca tgttcatggt
//
LOCUS       DBNGSH010000942         1575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1144206, whole genome shotgun sequence.
ACCESSION   DBNGSH010000942 DBNGSH010000000
VERSION     DBNGSH010000942.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1144206"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="AC16CY_21565"
     CDS             complement(<1..666)
                     /locus_tag="AC16CY_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3356454.1"
                     /translation="MSTRVEEQLRAEIVEAGRRLYARGFVASNDGNISARLDETRLIT
                     TPKSVSKGFMTPDMMVIVDYEGKKMSGDREASSELPMHLEIYRSRPDVNAVVHAHPPV
                     ATGFAVAGIPLTRAVLAEVVTTLGSIPIAEYGTPSTEELPAAVRKYIKAHDGLLLANH
                     GAVTCCRSVMTAYYKMETIEHFAKISLVARLLGREHLISRDEVARLQGLRGMYGIAAP
                     APLC"
     gene            complement(663..926)
                     /locus_tag="AC16CY_21570"
     CDS             complement(663..926)
                     /locus_tag="AC16CY_21570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356455.1"
                     /translation="MTRIALLAILLLAVAWAFWRLMDGVIEVLGGRPGSARGRAKAPA
                     VKLVRDPVCGTWVSPANARFLQRGSATHYFCSDECRVKFEKRS"
     gene            complement(923..>1575)
                     /locus_tag="AC16CY_21575"
     CDS             complement(923..>1575)
                     /locus_tag="AC16CY_21575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356456.1"
                     /translation="PHPIYWRPQLSMKSDSIAYVVAGICFGVILGWVIGTQQARRSTV
                     TPAAASPAASQTTGNRVQEPPPLNEGRVQELSATLKQNPKDVNATVQLGNLYFDAERW
                     DDAITWYRRALELDPDNPDASTDLGVSYYYTNRLDQALAQFERSLAMSPTHTKTLLNK
                     GIVLAFGKQDLKAAAVEWQKVVELAPNSPEGQAARRALDGIASAAHSNSGTTTGNP"
BASE COUNT          245 a          516 c          525 g          289 t
ORIGIN      
        1 acaaagcggc gccggcgcgg cgatcccgta cattccccga agtccctgca gtcgcgctac
       61 ttcgtctctc gagatcaggt gctcgcgacc gagcagtctc gccaccaggc tgatcttggc
      121 gaagtgctcg atggtttcca tcttgtagta cgcggtcatc accgaacggc agcacgtcac
      181 ggcgccgtgg ttggccagca gcaggccgtc gtgggccttg atgtacttgc gcaccgcggc
      241 gggcagctcc tcggtggacg gcgtgccgta ctccgcgatc ggaatgcttc ccagcgtcgt
      301 cacgacctcc gcgagcacgg cacgcgtgag agggatgccg gcgacggcga acccggtggc
      361 cacgggcggg tgcgcgtgga ccacggcgtt gacatccggc cggctccggt agatctccag
      421 gtgcatgggg agctcggacg aggcctcgcg atcgccggac atcttctttc cctcgtagtc
      481 cacgatcacc atcatgtccg gagtcatgaa gcccttggag acgctcttcg gagtggtgat
      541 gagccgcgtc tcgtcgagcc gggcgctgat gttcccgtcg ttcgaggcca cgaagccgcg
      601 cgcgtacaag cggcggccgg cttcgacgat ctccgcgcgc agctgttctt cgacgcgggt
      661 gctcacgatc gtttctcgaa cttcacacgg cactcatcgg agcagaaata gtgtgtcgcc
      721 gaaccgcgct gcaggaatcg tgcgttggct ggcgagaccc acgtgccgca taccggatcg
      781 cgcaccagct tgacggccgg tgctttggcc ctgccgcgag cgctgccggg ccggccgccg
      841 agcacctcga tcacgccgtc catgagccgc cagaacgccc aggcgacggc cagcaggaga
      901 atcgcgagta gcgcgatgcg ggtcacgggt ttcccgtcgt cgtgccggag ttcgagtggg
      961 cggccgacgc aatcccgtcg agcgcgcggc gggccgcctg gccctcggga ctgttgggcg
     1021 ccagctccac gaccttctgc cattcgactg ccgcggcctt cagatcctgc ttgccgaacg
     1081 ccagcacgat gcccttgttc aacagcgtct tggtgtgcgt gggactcatc gcgagagagc
     1141 gctcgaactg cgccagcgcc tgatcgagcc tgttcgtgta gtagtaactc actcccaggt
     1201 ccgtgctggc atcggggttg tccgggtcga gctcgagcgc ccgccggtac cacgtaatcg
     1261 cgtcgtccca ccgctcggcg tcgaaataga gattgccgag ttgaacggtc gcgttcacgt
     1321 ccttggggtt ctgtttcagc gtagcgctga gctcctgcac ccggccttcg ttcagcggcg
     1381 gcggctcctg caccctgttg cccgtcgtct ggctggcggc aggagaggcg gcggccgggg
     1441 tgacggtcga tcgcctggcc tgctgcgtgc caatcaccca tcccaggatg acgccgaagc
     1501 agatgccggc aacgacgtaa gcaatcgagt cggatttcat cgataactgg ggtcgccaat
     1561 aaatggggtg ggggg
//
LOCUS       DBNGSH010000943         1981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_635728, whole genome shotgun sequence.
ACCESSION   DBNGSH010000943 DBNGSH010000000
VERSION     DBNGSH010000943.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_635728"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="AC16CY_21580"
     CDS             complement(<1..397)
                     /locus_tag="AC16CY_21580"
                     /inference="COORDINATES: protein motif:HMM:NF042769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3356457.1"
                     /translation="MTRHQIGRRSSMAALLAVMGFVLAGSPAGQTPSVREHKDLVYAT
                     VDGKSLALDLYIPAGVQSPGLVVWVHGGAWRSGTKAQVQRQFFENGYATASLDFRQST
                     EARFPAQAHDIKAAIRFLRAKAKEYGYRTD"
     gene            524..1699
                     /locus_tag="AC16CY_21585"
     CDS             524..1699
                     /locus_tag="AC16CY_21585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3356458.1"
                     /translation="MHLSPRSSHLRPTAVNTILAEIRQAQARGRIIVSLMRGEPDFDT
                     PLHIADAAARAMKAGRTRYPDNRGELVLREAVAAKLSRDNGVTYDPASEILVTTGATL
                     GIYAALMALLNEGDEVLLADPVYDAYNSPVVLAGGRPVPVPATIEDGRFVVTAAAIER
                     ALTPRSRALLLNTPWNPVGTVFRASELHAIGDVVLRRDLALISDEIYEAITYGDARHV
                     SPSSLSAELRARTILVNSLSKTYAMTGWRVGYCAAPAPVIDSMFLVLQQSSRGPSTFV
                     QDAAAAALSGPQSSVNAMRDEYTRRRSQVLSALAGINRVRVLPPEGGFFAMVDARDTK
                     VTSNEIRRRLLNEAGVAVVHGAAYGPAGEGTLRVSFASGGDTLARGLERLRAGLEAL"
     gene            1696..>1981
                     /locus_tag="AC16CY_21590"
     CDS             1696..>1981
                     /locus_tag="AC16CY_21590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3356459.1"
                     /translation="MSAELARALADERIEDVRFDSVSRALYSTDASVYQIEPVGVALP
                     RSRDELRRIVQVCARLGCPITMRGGGTSQAGQAVGHGLVVDTSKHLNAVLE"
BASE COUNT          320 a          685 c          643 g          333 t
ORIGIN      
        1 gatccgtccg gtacccgtac tccttggcct tcgcgcgcag gaagcggatg gcggccttga
       61 tgtcgtgggc ctgggccggg aagcgcgctt cggtggactg gcggaagtcc aggctcgccg
      121 tcgcgtaccc gttctcgaag aactgtctct ggacctgtgc cttggtgcca ctgcgccacg
      181 cgccgccgtg aacccagacc accagcccgg gcgactgaac gcctgcgggt atgtacagat
      241 cgagagccag gctctttccg tcaaccgtcg cgtagaccag atctttgtgt tcgcgaaccg
      301 acggcgtctg tcccgcggga gatccagcga gcacgaagcc catgacggcc agcagcgcgg
      361 ccatcgacga gcggcgtcca atctgatgtc tggtcatcgg cggggccctc acagttcatg
      421 gtcatgcgcg gaaccagcca cgcagggtgg agtgtagaac atcgcacttc ccacttcaaa
      481 cgtccttttg aactttgcac cttccacttt agacttgcag accttgcacc tttcaccgcg
      541 ctcctcgcac cttcgcccca ctgccgtcaa cacgatcctc gccgagattc gccaggcgca
      601 ggctcgagga cggatcatcg tgtcactgat gcgtggcgag ccggacttcg acacgcctct
      661 tcacatcgcc gacgcagccg cccgtgccat gaaggcgggc cgtacgcgct accccgacaa
      721 tcgcggagag ctggtccttc gcgaagcagt ggcggcgaag ctttcgcgcg acaacggcgt
      781 gacctatgac cccgcatctg aaatcctcgt gacgacggga gcgacgcttg gcatctatgc
      841 ggccttgatg gcgctcctga acgaaggaga cgaggtgctg ctggcggacc cggtctacga
      901 cgcctacaac tcccccgtcg tgctcgccgg cggccggccg gttcccgtgc ccgcgacgat
      961 cgaagacggc cggttcgttg tgactgccgc cgcgatcgag agagccctga cgccccgatc
     1021 gcgagcgctg ctgctgaaca cgccctggaa tccggtcggc acggtgtttc gagcctcgga
     1081 gcttcacgca atcggcgacg tcgtcctgcg acgcgatctt gcgctcatca gcgacgagat
     1141 ctacgaggcg atcacctacg gcgatgcgcg ccacgtctca ccatcctcgc tgtcggccga
     1201 gctgcgcgcg cgcacgatcc tcgtcaacag tctgtcgaaa acgtacgcga tgaccggatg
     1261 gcgcgtgggt tactgcgccg cgcccgcgcc cgtcatcgac agcatgttcc tcgtgctgca
     1321 gcagtcgagc cgtggaccgt ccacgttcgt ccaggacgcg gcggcggccg cgctatcggg
     1381 cccccagtcg tcggtgaacg cgatgcggga cgaatacacg cgccgccgat cgcaagtgct
     1441 gagcgctctc gcgggcatca accgggtgcg cgtgcttcca cccgagggcg ggttttttgc
     1501 catggtggac gcgcgagaca cgaaggtcac ctccaacgag atccggcgcc ggctcctgaa
     1561 cgaggctggt gtggccgtcg tccacggcgc cgcctacggg cccgcaggcg aaggcacgct
     1621 tcgcgtctcg ttcgcaagcg gcggcgacac gctcgcccgc ggcctggaac ggctgcgtgc
     1681 cggactcgag gcgctgtgag cgccgaactg gcgcgggcgc tggcagacga gcgcatcgaa
     1741 gacgtccggt tcgacagcgt gtcccgggcg ctgtactcca ccgatgccag cgtctaccag
     1801 atcgagccgg tcggcgtggc gctcccacgg tcgcgcgacg agctccggcg gatcgtgcag
     1861 gtctgcgcgc ggctcggctg cccgatcacg atgcgcggag ggggcacgtc acaggccggc
     1921 caggccgtcg gtcacggcct cgtcgtcgat acgtcgaagc acctgaacgc cgtcctcgag
     1981 g
//
LOCUS       DBNGSH010000944         1632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1144814, whole genome shotgun sequence.
ACCESSION   DBNGSH010000944 DBNGSH010000000
VERSION     DBNGSH010000944.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1144814"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="AC16CY_21595"
     CDS             <1..245
                     /locus_tag="AC16CY_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3356460.1"
                     /translation="IVNQGTIYLCLVRLVQKKWISAKWGTSENNRRAKFYAITSAGRA
                     QLRAETENWARISGVIGRVLQPGGGLPTGAPAKAGS"
     gene            249..>1632
                     /locus_tag="AC16CY_21600"
     CDS             249..>1632
                     /locus_tag="AC16CY_21600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356461.1"
                     /translation="MRALFHTLVSRLRGFVRRAAADDEFDQELSAHLTMAEEDKVRRG
                     MSPSEARREARLELGGVTQLREAARATRGLPWIDSFWLDAKLGGRMLRKSWGLTLVGG
                     LAMAITIGLGASIFTIWDAVSGTRLPLDEGDRVVAIQTLNRGTQQVNSLTPLADFRRW
                     RETLTSVAQVSAMRPRNLTVVTRDGALEPVRAAEITASAFQLARVQPLMGRPLIDSDE
                     REGAEPVAVIGHRLWEEAFASDPSVIGQRLQVGDTPHVVVGVMPRGFRFPVNQRLWMP
                     LRTDRVSEAGPDSDVFVFARLAVGASQERAQVEVATIGLLPRNQARETAQAEPRVVPY
                     AAGFLPVVHGNRWLTGVIFLVGALLLVPPCANVAILVYARTVTRREEFAARTALGASR
                     ARIVMQVFVELLVLAAGAGILGFFIARQYSGQLIGMVVPMMGPGSVPFWMDFTPSLAT
                     VLWVAGLSVLA"
BASE COUNT          232 a          542 c          584 g          274 t
ORIGIN      
        1 tcatcgtcaa tcaggggacg atctatctgt gtctcgtgcg cctcgtgcag aagaagtgga
       61 tctcggcgaa atggggcacg tccgagaaca accgcagggc aaagttctac gcgatcacga
      121 gtgccggacg cgcgcagctg agagcggaga ccgagaactg ggcgcgcatt tccggcgtga
      181 tcggccgcgt cctgcagccg ggcggcggcc tgcccaccgg agctcccgcg aaggcgggaa
      241 gctgacccat gcgagccctg ttccacacgc tggtgtcgcg cctgcgcggg ttcgtgcgcc
      301 gcgccgcggc tgacgacgag ttcgaccagg agctttcagc gcatctgacg atggcggagg
      361 aggacaaagt ccgccgcggc atgagcccgt cggaggctcg gcgcgaggcg cggctggagc
      421 tgggcggagt gacgcagttg cgcgaggcgg ctcgggcgac gcggggcctg ccatggatcg
      481 attccttctg gctcgacgcc aagctcggcg ggcgcatgct gcgcaagtcg tgggggctga
      541 ccctggtcgg tgggctggcg atggcgatca cgatcgggct cggcgcctcc atcttcacca
      601 tctgggacgc cgtctcgggc acgcggctcc cgctcgacga aggggatcgc gtggtggcga
      661 tccagacgct gaacaggggt acgcagcagg tcaacagtct cactccgctc gccgacttca
      721 ggcgctggcg tgagacgttg acgtcggtcg cgcaggtcag cgcgatgcga ccgcgcaacc
      781 tcaccgtcgt gaccagggac ggcgcgctcg agcccgtgcg cgccgccgag atcaccgcgt
      841 cagccttcca gcttgcacgg gtccagccgc tgatgggacg gccgctcatc gacagcgacg
      901 aacgggaggg cgcggagccc gtcgccgtca tcggccatcg cctctgggag gaggcgttcg
      961 cgtccgatcc atcggtcatc ggccagcgcc ttcaggtcgg cgatacgccg cacgtcgtcg
     1021 tcggcgtgat gccgcgcggc ttccggtttc ctgtcaacca gcgcctctgg atgccgctcc
     1081 ggaccgatcg ggtcagcgag gcggggcctg acagcgatgt cttcgtcttc gcgcggctcg
     1141 ctgtgggagc atcgcaggag cgtgcgcagg tggaagtggc caccatcggc ctgttgccac
     1201 ggaatcaggc ccgggagacg gcgcaagcgg aaccgcgggt cgtgccctac gccgccgggt
     1261 tcttgcccgt cgttcatgga aaccgctggc tcacgggcgt catcttcctg gtcggtgcac
     1321 ttctcctggt cccgccgtgc gccaacgtcg ccatcctggt gtacgcgcga acggtcacga
     1381 ggcgcgagga gttcgccgcc cgcaccgcgc tcggcgccag ccgcgcccgc atcgtcatgc
     1441 aggtcttcgt cgagttgctc gtgctcgccg ccggcgccgg cattctcggc ttcttcatcg
     1501 cccggcagta cagcggccag ttgatcggga tggtcgtgcc catgatgggg cccgggagcg
     1561 tgcccttctg gatggacttc acgccgtcgc tcgcgaccgt cctctgggtg gcaggtttga
     1621 gcgtgctcgc tg
//
LOCUS       DBNGSH010000945         2437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4370924, whole genome shotgun sequence.
ACCESSION   DBNGSH010000945 DBNGSH010000000
VERSION     DBNGSH010000945.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4370924"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="AC16CY_21605"
     CDS             <1..240
                     /locus_tag="AC16CY_21605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356462.1"
                     /translation="DQRDKGAPPFNIWAIGLTVPLEAGDANPRAYDRLSDIAKDVVNA
                     RIYLGIHFRFADTQARSQGRRVAGYAFRNFLEPVE"
     gene            352..1062
                     /locus_tag="AC16CY_21610"
     CDS             352..1062
                     /locus_tag="AC16CY_21610"
                     /inference="COORDINATES: protein motif:HMM:NF016301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HIR3356463.1"
                     /translation="MTPPVRRRALLLLAASIAILIPGLFLPVLSIRGLLTREGIAAVA
                     PQMLDKGLNDETMATLTSMLNPQIVTMVQITGADLRKTIIDRLTPQLTTALQKNVGDV
                     EVYQQTRSIIGSIENLYDVGSPVPATLILLFSVVVPFTKAALVGWAVLVSGDERGRRT
                     MRFVEAIAKWSMADVFVVGLFIAYLAAEATQQAEGSTALGLVAFDARFGPGFYWFAAY
                     CLLSLASQQYTRRAMERE"
     gene            1098..>2437
                     /locus_tag="AC16CY_21615"
     CDS             1098..>2437
                     /locus_tag="AC16CY_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356464.1"
                     /translation="MDCGRRIDSIAVALAVVLAIAVPATAGEQAPQAPAGAGPQGRGA
                     GGAQGQAGAPGRGRGGDPNAGAPYTPAAGAKDLRAVLFNWFWHQGMLKGTDERDMVAM
                     LEYQAKGGTIQVDGQPCTLSRLRESTNFQTFSQRVDYTCTRPNKQAVSNIEVVSWQYA
                     WNEDTRGAEIGGHKGKATAMPATVQERLIRLWASSQGAPKSALAGTMDTWTLGANPGT
                     VIPDGVTKLGNTSVSFDAAGKPVVTFPIPGVAGATATATLDAKYMTEKVVVSHGGTTT
                     EFTYSNYQDWNNPLHKIEVFYAGRLIERKNGAVVRDLTTRETETGTVYVVAPVPPSVQ
                     KAMNVTTKAPTIIFAKAEPPTNTSAPTPRLGKVPDLTGNWAVAQNGWIGNYMGNGGRR
                     CGPTQVQPCNRATNQTEDFALYSPSRFGQFGRPLYKPEHWDKVIELDMWTNKYDP"
BASE COUNT          446 a          798 c          816 g          377 t
ORIGIN      
        1 gaccagagag acaaaggggc gcccccgttc aacatctggg ctatcggcct gaccgttccg
       61 ctcgaagctg gcgacgccaa tccgcgtgcg tacgacaggc tgtccgacat cgccaaggat
      121 gtggtcaacg cgcgcatcta cctggggatt cacttccgct ttgccgacac ccaggcgcga
      181 agccagggcc ggcgcgtcgc cggatacgcg ttccggaact tcctcgagcc ggtcgagtag
      241 gagcacggcc gcggccatcg atcgcacatg cagagagggc agcccgaggc tgccctcttt
      301 gccgcctcgc accgtcgtac gccgggtccg gacgagtatg atctggcttc gatgacccca
      361 ccagtccgcc ggcgcgctct cctgctcctc gcagcgtcga tcgccatcct gatccccggc
      421 ctgtttctgc ccgtcctcag catcaggggc ctcctgacgc gcgaaggcat tgccgccgtc
      481 gctccgcaga tgctcgacaa gggcttgaac gacgaaacga tggcgacgct cacgtcgatg
      541 ctgaaccccc agatcgtgac gatggtgcag atcaccggcg ccgatctgcg caagacgatc
      601 atcgatcggc tgacgccgca gttgacgacg gcgctgcaga agaacgtggg cgacgtcgag
      661 gtctaccagc agacgagaag catcatcgga tcgatcgaga acctgtacga cgtcggcagc
      721 ccggtccccg caaccttgat cctgctgttc agcgtcgtcg tgccgttcac gaaagcggcg
      781 ctcgtcggct gggcggtcct cgtcagcggc gatgaacgcg ggcggcgcac gatgcgcttc
      841 gtcgaagcca tcgcgaagtg gtcgatggcc gacgtcttcg tcgtcgggct cttcatcgcc
      901 tacctcgccg cggaggccac gcagcaagcg gaggggagca cggcgctggg gctggtggcg
      961 ttcgacgccc ggttcggccc cggattctac tggttcgcgg cctattgcct gctctcgctc
     1021 gcgtcgcagc agtacacgcg tcgggcgatg gaacgggagt aatatttgcc aaagtcagga
     1081 tacgcgggag ggccatcatg gattgtggtc gacggattga ctcgatagcg gtcgcgctcg
     1141 cggtcgtcct cgcgatcgcc gtgccggcga cggccggcga gcaagctcct caggcgcctg
     1201 ccggcgcggg tccacagggc cgcggtgcag gcggcgccca gggtcaggcc ggcgcgccgg
     1261 gccggggtcg tggcggtgac ccgaacgcgg gtgcgcctta cacgcccgcc gcaggcgcca
     1321 aggatctaag ggctgtactc ttcaactggt tctggcacca gggcatgctg aagggcaccg
     1381 acgagcgcga catggtcgcg atgctcgagt accaggccaa gggcggcacc attcaggtgg
     1441 acggtcagcc gtgcacgctc tcgaggcttc gcgagagcac gaacttccag acgttcagtc
     1501 agcgcgttga ctacacctgc acgcgtccga acaagcaggc ggtgtcgaac atcgaagtgg
     1561 tgagctggca gtacgcgtgg aacgaggaca cgcgaggcgc cgagatcggc gggcacaagg
     1621 gcaaggccac cgcgatgccg gccaccgtgc aggagcggct cattcgcctc tgggcgagtt
     1681 cgcagggcgc gcccaagtcc gcgctggcgg gaacgatgga cacctggacc ctgggcgcca
     1741 acccgggcac ggtcatcccc gacggcgtga cgaagctcgg caacacctcc gtgtcgttcg
     1801 acgccgcggg caagccggtt gtgaccttcc cgatccccgg cgtcgcaggc gccaccgcca
     1861 ccgcgacgct cgatgcgaag tacatgaccg agaaggtcgt ggtgagtcat ggcgggacga
     1921 cgaccgagtt cacctacagc aactatcagg actggaacaa cccgctgcac aagatcgagg
     1981 tgttctacgc cggccgattg atcgagcgga agaacggcgc ggtggttcgc gacctgacga
     2041 cgcgcgagac ggagacgggc actgtgtacg tggtggcgcc cgtgccgccc agcgtgcaga
     2101 aggcgatgaa cgtcaccacg aaggcaccca ccatcatctt tgcgaaggcg gagccgccca
     2161 cgaacacgtc ggcgcccact ccgcggctcg gcaaggtccc cgatctgacc gggaactggg
     2221 cggtcgcgca aaacggctgg attgggaact acatgggaaa cggcgggcgg cggtgcggtc
     2281 cgacgcaggt acagccctgt aaccgagcca ccaatcagac cgaggacttt gcgctctact
     2341 ccccatcccg gttcggccag ttcggtcgtc cgttgtacaa gccggagcac tgggacaagg
     2401 tcatcgagct cgacatgtgg acgaacaagt acgatcc
//
LOCUS       DBNGSH010000946        12097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3691869, whole genome shotgun sequence.
ACCESSION   DBNGSH010000946 DBNGSH010000000
VERSION     DBNGSH010000946.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12097
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3691869"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="AC16CY_21620"
     CDS             <1..371
                     /locus_tag="AC16CY_21620"
                     /inference="COORDINATES: protein motif:HMM:NF015279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356465.1"
                     /translation="VSASLALRVPAPTDWTPPIVKTQALSGEGLDLLWSEVGRFRTWA
                     AEHRPAQRRQRHESRLRDLLARAFLRQVDATIPAGEFDRFVAAVDARESDPYSAAAEI
                     MRRVQQGAQGSGPRDQGPRE"
     gene            368..1630
                     /gene="mce"
                     /locus_tag="AC16CY_21625"
     CDS             368..1630
                     /gene="mce"
                     /locus_tag="AC16CY_21625"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR3356466.1"
                     /translation="MRAILDHVGIAVQDVQQALAFYRDALGLDVHAPEEVATERVRVH
                     AVPVGGPEIELLEATAADSTIAAFVRKRGPGLHHVTLRVEDLRAALAQLRARGVRLVG
                     EAPRKGAEGSLVAFVHPSDAHGVFIELKQRASPPVRLDVRSLELGDLRLTLLHDGPFR
                     LDGGAMFGVVPRPLWETVSPPDERNRIQLAMRPLLVEASWGRMIVDCGAGDKMSPKQV
                     DIYALDRSRHLDHALDTVGLTSDSIDFALATHLHFDHFGGATTQAGGSLRPRFANARY
                     LIRAAEWEDATHPHERNRASYLQENFVPLAGAGVIDFFADDGPIKPGVRVFRTGGHTG
                     QHQIVYVESRGRTAVFVADMIPTTAHLQDPWIMGYDLFPMDTLAFKKRFIREAVEREY
                     LILFEHDPRVAAGYVREKDGKRFVEQVL"
     gene            1715..2584
                     /gene="mtnP"
                     /locus_tag="AC16CY_21630"
     CDS             1715..2584
                     /gene="mtnP"
                     /locus_tag="AC16CY_21630"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HIR3356467.1"
                     /translation="MEKVQIGIIGGSGLYDMAEVTHRSEVTITTPFGEPSGPYLLGTL
                     RGKRVAFLARHGAGHRLLPSELNFRANIFGMKTLGVEYILSASAVGSLKQEYKPLDIV
                     IPDQFIDRTKGRVSTFFGRGLAAHVGFAHPFCRILSGLVVASGQHAGATVHAGGTYVC
                     MEGPQFSTLAESNLYRSWGADIIGMTNLQEAKLAREAEMCYATIALVTDYDCWHPDHD
                     SVTVEMIISNLVHNAATAQQIIAHAVDRLPFERSCECATALKFALITRPDMIPAQLRQ
                     DLAPLVGKYLQTT"
     gene            2746..3738
                     /locus_tag="AC16CY_21635"
     CDS             2746..3738
                     /locus_tag="AC16CY_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HIR3356468.1"
                     /translation="MNSATFDVTAIGNAIVDVIAQADDALLAAHNLPKGAMNLIDAAM
                     AEKLYSIMGPGKEASGGSAGNTIAGIAMLGGKTAYIGKVADDQLGSIFTHDIRAAGVT
                     YDTRPLVGGLPTARSLIFVTPDAQRTMQTFLGACTQLGPEDVNLNHVSASKVVYMEGY
                     LWDLPRAKEAMLGAAMKAKEHGVKVSLTLSDAFCVGRFRDEFLELAEKYVDILFANES
                     EILSLYQTDDFDEALQQVRKHCDIAALTRSEKGSIVVNGDEVHVIDAVKGVKVVDTTG
                     AGDAYAAGFLYAYTQGADLKKAGRLGGAMAAQVISQYGPRSEKADVLRIKASVV"
     gene            complement(3745..5229)
                     /gene="guaB"
                     /locus_tag="AC16CY_21640"
     CDS             complement(3745..5229)
                     /gene="guaB"
                     /locus_tag="AC16CY_21640"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR3356469.1"
                     /translation="MSDLLDLVRGCTFDDFLFTPQYGVIERRDPSTIDLSCRLSEHLT
                     LKRPIVSANMDTITRAEMAIAIAEEGGIGIIDRGFRAGDIEPQVREVERVKRRQHGII
                     GDPYTIGPEAPLTDASAVMQRTGVGTLVVVDEARRVLGLLTTRDMRFADPSASVAARM
                     TPRDRLVVRQGAIDAPAAEAVMREHKVKKLPLVDGDLRLIGLVTAKDLVKQEQLPFAT
                     RDAHGRLRVGAAIGAKGDYLERATELLRAGADLIVIDIAHGHSAIMARAIDAFRSRFG
                     DAELVAGNVATADGVRFLVERGANAVKVGIGPGGGCTTRLNTNFGVPQVEALAAGRAA
                     AGGRVPLIADGGIRRDGSIVQALLFGGDTVMLGSVFAGTLETPGDTVQKPVVIPESNK
                     TVKVPFKVFRGMASLQAIVDRLDVEDAETADVEALGAEGMEVSVPARGSVRPIVRDMV
                     KHLCSAISYGGAGSLSELQEKFRQDPRRYTIRLSDPARRESFDR"
     gene            5286..5771
                     /locus_tag="AC16CY_21645"
     CDS             5286..5771
                     /locus_tag="AC16CY_21645"
                     /inference="COORDINATES: protein motif:HMM:NF021384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HIR3356470.1"
                     /translation="MWVLLLGVAATAWVALLVVAPYLPAYAGAVLYGTGSLVCHQISE
                     RSFHVAGSQLPVCARCLGLYAGAWLGVIGRLWWGRVPALDSSRTARAAAVLAAVPTAV
                     TWTFEVVGVWNPSNATRAIAGLPLGAVVGLVVAGAVATLHYDECVPPRPTAPRPPPPS
                     I"
     gene            5720..6115
                     /locus_tag="AC16CY_21650"
     CDS             5720..6115
                     /locus_tag="AC16CY_21650"
                     /inference="COORDINATES: protein motif:HMM:NF042755.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HIR3356471.1"
                     /translation="MRAAAADRAQAASTVYLICAAAWLLPGAGHLWLGRRQKGLVFLV
                     ALTLMFGFGLWLEGRLFPFEVTQPLIVLAAIADLGMGAPYLAAKAFGAGAGRVVAITY
                     EYGNTFIIVAGLLNMLVVLDVFDIAKGRK"
     gene            6112..6285
                     /locus_tag="AC16CY_21655"
     CDS             6112..6285
                     /locus_tag="AC16CY_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356472.1"
                     /translation="MTSHLGLMVIFAAFVSVVFAVLSRDEPRDQMVLGARMFGGFVFA
                     GVLIGWLLYPLPF"
     gene            6348..6686
                     /locus_tag="AC16CY_21660"
     CDS             6348..6686
                     /locus_tag="AC16CY_21660"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR3356473.1"
                     /translation="MALIFFVSSQPSAPLPESVSDKGAHLLAYSVLGVLSVRAAGGGL
                     PCRVTVPIALTALAITSGYGALDEWRQGVVPGRSQDIADWYADTAGAVIGIGVCWAWG
                     MMRALGRRSP"
     gene            6683..7162
                     /gene="ribH"
                     /locus_tag="AC16CY_21665"
     CDS             6683..7162
                     /gene="ribH"
                     /locus_tag="AC16CY_21665"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR3356474.1"
                     /translation="MKELQGSKRAAGCRFAIVVSRFNAEITEGLLQGACSALADAGVG
                     SEDVTIVRVPGAFEIPLVARRLAKTGRFDAVVCLGCLIKGETMHFEYIASAVSQGIMD
                     AGADTGVPVTFGVLTALTDEQAASRALGGADNKGREAAMAAVEMATLGRRLNEEGQR"
     gene            7159..7623
                     /gene="nusB"
                     /locus_tag="AC16CY_21670"
     CDS             7159..7623
                     /gene="nusB"
                     /locus_tag="AC16CY_21670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3356475.1"
                     /translation="MKAGAVPRPAVRQRAESRHRARETALQMLYQWEVGRTPIEEVCR
                     TFWSDGPSTVRLGDALRAFAVALATGVATHTTGLDTLIVDAAEHWRIERMNVVDRLIL
                     RLAVYELLYEPDTPGKVVINEALELARTFSAGESTRFINGMLDAIRRTLGRE"
     gene            7620..9128
                     /gene="lysS"
                     /locus_tag="AC16CY_21675"
     CDS             7620..9128
                     /gene="lysS"
                     /locus_tag="AC16CY_21675"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR3356476.1"
                     /translation="MSAEESEQGGQRRANFDELKKLGVDPYPRRFEGGVSIGSLVAEH
                     GARSGEELESLQIRTRTAGRILAIRSFGKANFLQLSDGHARVQVYVRQDSVPELDFKV
                     FKLLDIGDWVGVEGRLFRTKTNEFSVYASSMQFLSKCLLPLPEKWHGLQDVEIRYRQR
                     YLDLIVNPDSRRVFEVRSRLVTSIREFLTRRGFLEVETPMMQPIAGGALARPFVTHHN
                     TLDMRLYMRVAPELYLKRLTVGGIEKVFEINRNFRNEGISTQHNPEFTMLEFYWTYAD
                     YNDLMTLTEDMLQQVARAATGADEATFGEHVISFRPPFRRLSLREGARAAASARLGTG
                     VTDRELRTRESAAALASRLGIEVAPGSGPGKIAAAIFEALCEADLVQPVFVYDFPTEV
                     SPLSKQKSDDPDTVERFELYVAGFEIANAFSELNDPVEQRRRFEHQLSGRGDRHEEAH
                     AMDEDYIRALEYGLPPTGGEGIGIDRLVMVLTNSPSIRDVILFPLMRPRDGG"
     gene            complement(9166..10047)
                     /locus_tag="AC16CY_21680"
     CDS             complement(9166..10047)
                     /locus_tag="AC16CY_21680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR3356477.1"
                     /translation="MTPSLACGRRRAWAGTILSISLSAALGAQERPADFKTLYESADY
                     DQALVLLASLDTPDAHRYKALCLLALGRNQEAAAAVSALVTASPLFVASADDAPPRLV
                     QLVDETRRKMLPSIARRAFADGREHFAAKRNEEAERQFSLVLNLVADPAFVNATDAQD
                     LKTLAQGFIDLARAASAAQAAQVASKPAAPPAETVREAAVAPRPVKVIPAAPIRQRIP
                     PIPAALQGRVGPVLSLVVEIDTEGKVTSATVKQSSQPVYDQIVLGAVRDWQYTPASLN
                     GQPIGSEHLVTIKIASP"
     gene            complement(10044..11153)
                     /locus_tag="AC16CY_21685"
     CDS             complement(10044..11153)
                     /locus_tag="AC16CY_21685"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3356478.1"
                     /translation="MAQTPLRTDAVATFPDRSAVPPAQGDEDATLEFAAESKPVPEVP
                     QAASARKWIIAVTLAVVTVTAAAAGAVWAWPDSIGVAGAASGSLRVESDPPGAEVRLN
                     GEVRGTTPLSLTLPVGAYTLAVRQGSNVKQLPVEIAKGGGRDFHISFTEVAPPVVPAT
                     AGGLQVTSDSPDSVVSVDGVERGRTPLTVRNLAAGRHEVVVRTAGTTYRRTVQVEPGA
                     TASLVIGGTAPSTSWGWISLKSSFVIQVLEGGRLIGTSEIDRIMLPPGNHDLEFVSEP
                     FGLRSASRVSVRAGQAAPVSVNMPEVPMNINAIPWAEVFINNTRIGDTPLANVTLPPG
                     DHVIVFRHPQLGEKRQAVRVTLKESPRISVDMRSR"
     gene            11327..>12097
                     /locus_tag="AC16CY_21690"
     CDS             11327..>12097
                     /locus_tag="AC16CY_21690"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356479.1"
                     /translation="MSFELFVALRYLLASRQHSSISRVSLFSTIGVAVGVTALVVALA
                     LMTGLQGELRSRILGSTAHVYVWKAGGLDDYQADVARLRALPGVAGAGPAIVDKAIVR
                     SDRESAFVTLKGIDPVLESQVTDIDRTIKQGSVAALSNLGEEDRPGVLLGIDLAQHLE
                     VKVGDYVSLLTLAGIGSPMGRMPFPRRLRVAGIYQLGLYEYDSAYGFVTIDVAKRLFA
                     KTQPDLIQLRVDDIWSAAAIAKSVPERLSTDYIAEDWSE"
BASE COUNT         1949 a         3938 c         4187 g         2023 t
ORIGIN      
        1 cggtctccgc gagcctcgcg ctgcgtgtcc cggcgcccac ggactggact ccgccgatcg
       61 tgaagacgca ggcattgtcg ggagaagggc tggacctgtt gtggagcgag gtcggacggt
      121 tcaggacgtg ggcggccgag caccggccgg cgcaaaggcg ccaacggcac gagtcgcgtc
      181 tccgcgatct gctcgcacgt gcctttctcc gtcaggtgga cgcgacgatc ccggcggggg
      241 agttcgatcg gttcgtcgcc gccgtcgatg caagagaaag tgatccatac tcggccgccg
      301 ccgagatcat gaggcgtgtg caacaaggag cccagggctc agggcccagg gaccagggac
      361 caagggaatg agagccatcc tcgaccacgt cggcatcgcc gtgcaggatg tccagcaggc
      421 gctcgccttc tatcgcgatg cgctcggcct cgacgtgcat gcgccggagg aggtggcaac
      481 cgagcgtgtg cgcgtacacg ccgttcccgt gggagggccc gagatcgagc tgctcgaagc
      541 gaccgctgcc gattcgacga tcgccgcctt cgtgcggaag cgcggcccag ggctccacca
      601 cgtcacgctg cgtgtcgaag atctgcgcgc ggcgctcgcg cagctccggg cccggggcgt
      661 acggctggtg ggcgaggcac cgcggaaggg tgccgagggt tcgctcgtgg cgttcgtcca
      721 tccgtccgat gcgcacggcg tgttcatcga gctgaagcag cgcgcatcgc cgccggtgcg
      781 gctcgacgtc aggagcctcg agctcggcga tctccggctg accctgctgc acgacggccc
      841 attcaggctc gatggagggg cgatgttcgg cgtcgtgccg cgccccttgt gggagacggt
      901 gtcgccaccc gacgaacgca acaggatcca gctggccatg cggcccctgc tcgtcgaggc
      961 gtcgtggggg cggatgatcg tcgactgtgg cgccggcgac aagatgagcc cgaaacaggt
     1021 ggacatctac gccctcgacc ggagccgcca cctcgatcac gcgctcgaca ccgtcgggct
     1081 gacatcggac tcgatcgact tcgcgctcgc gacgcacctg cacttcgatc acttcggcgg
     1141 cgcaacgacg caggcaggcg ggagtcttcg tccgcggttc gccaacgcgc gatatctcat
     1201 cagggcggcc gagtgggagg acgcgacgca cccgcacgaa cgcaatcgtg cgagctacct
     1261 ccaggagaac ttcgttccgt tggcgggcgc gggggtcatc gacttctttg cagacgacgg
     1321 gccgatcaag ccgggcgtac gggtgttccg cacgggcggc cacactggcc agcaccagat
     1381 cgtgtacgtc gagtcccgcg gccggacggc cgtgttcgtc gccgacatga tccccacgac
     1441 cgcgcacctg caggacccgt ggatcatggg atacgacctg ttcccgatgg acacgctggc
     1501 gttcaagaag cggttcattc gtgaggccgt cgagcgggag tacctgattc tgttcgaaca
     1561 cgatccgagg gtcgccgcgg ggtatgtcag ggagaaagac gggaagcggt tcgtcgagca
     1621 ggtgctctag caggggacag ggggcagggg gcagggggca gggggcagag ggcagggggc
     1681 agagggcagc gggcgaaggc gagggggcag tagcttggaa aaagtgcaga ttggcatcat
     1741 cggtggcagc ggcctctatg acatggccga agtcacccac cgcagcgagg tcacgatcac
     1801 cacgccgttc ggcgagccgt ctggcccgta tctgctcggc acgcttcgag gcaagcgagt
     1861 ggcttttctg gcgcggcacg gcgcgggcca caggctgctg ccgtcggagt tgaacttccg
     1921 cgccaacatc ttcggcatga agacgctcgg cgtcgaatac attctctctg cgagtgcggt
     1981 cggcagcctg aagcaggagt acaagccgct cgacatcgtg atccccgacc agttcatcga
     2041 tcggacgaaa gggcgcgtca gcacgttttt cgggcgcggt ctggcggcgc acgtggggtt
     2101 cgcgcacccg ttctgccgga ttctgagcgg cctcgtcgtc gccagcggcc agcacgcggg
     2161 cgccacggtc cacgcgggcg ggacctacgt gtgcatggaa gggccgcagt tctcgacgct
     2221 ggccgaatcg aacctgtatc gatcctgggg agcggatatc atcgggatga cgaacctgca
     2281 ggaggccaag ctcgcccgcg aggccgagat gtgctacgcc accatcgcgc tggtgaccga
     2341 ctacgattgc tggcacccgg accacgactc ggtcaccgtg gagatgatca tctcgaacct
     2401 ggtccacaac gccgcgaccg cgcagcagat catcgcccac gcagtggatc gtcttccgtt
     2461 cgagcgctcg tgcgagtgcg cgaccgctct caagttcgcg ctcatcacca ggccggacat
     2521 gattccggcc cagctcagac aggacctggc gccgctcgtc ggcaagtatc tgcagacgac
     2581 ttagtggtat gtctttcgag gagtactacc ggccgctccg ttgtcgcggc cggattctcg
     2641 ctgtcggagg ccggttggga ctggtactcc ttcagtggat acactccact agaacgccgc
     2701 gcccatagaa aaccgcccag ccggcctacc aaggagagac ctactgtgaa cagcgccacg
     2761 ttcgatgtca ctgcgatcgg gaacgccatc gtcgacgtca tcgcccaggc cgacgacgcc
     2821 ctgcttgccg cacataacct gccgaagggc gcgatgaacc tgatcgacgc cgcgatggcc
     2881 gagaagctgt acagcatcat gggacctggc aaggaggcgt ccggcggatc ggcggggaac
     2941 acgattgcgg gcatcgcgat gctcgggggg aagaccgcct acatcggcaa ggtggctgat
     3001 gatcagctcg gctcgatttt cacgcacgac attcgcgctg ccggcgtcac ctacgacacg
     3061 cggccgctgg tcggagggct cccgaccgcc cgcagcctga tcttcgtgac gccggacgcg
     3121 cagcggacga tgcagacgtt cctcggcgcg tgcacgcagc tcggtccgga ggacgtcaac
     3181 ctgaatcacg tcagcgcgtc gaaagtggtc tacatggagg gctacctgtg ggacctgccg
     3241 cgcgcgaagg aagcgatgct cggcgccgcg atgaaggcca aggagcacgg agtgaaagtg
     3301 tcgctcacgc tctcggacgc gttctgcgtc gggcgcttcc gcgacgagtt cctcgagctc
     3361 gccgagaagt acgttgacat ccttttcgcg aacgaaagcg agatcctgtc gctctaccag
     3421 accgacgact tcgacgaggc gttgcagcag gtgcgcaagc actgcgatat cgcggcgctg
     3481 acgcgcagcg agaaaggctc gatcgtggtc aacggagacg aggtccacgt catcgacgcg
     3541 gtgaagggcg tcaaggtcgt ggatacgacc ggcgccggcg acgcctacgc tgcagggttt
     3601 ctctacgcct acacgcaggg ggcggacttg aagaaggcgg gcaggctcgg cggagcgatg
     3661 gcggcgcagg tgatcagcca gtacgggccg cgttccgaga aggccgacgt cctgaggatt
     3721 aaggcgtcgg tcgtgtagcc cgtttcagcg atcgaacgac tcgcggcgcg ccgggtcgga
     3781 caggcgaatc gtatagcgcc gtgggtcctg gcggaacttc tcctggagct ccgacaggct
     3841 gccggccccg ccgtagctga tcgccgagca gaggtgcttc accatgtcgc ggacgatcgg
     3901 cctcaccgat cctcgcgccg gcacgctgac ctccatgccc tcggccccca gcgcctcgac
     3961 gtcggccgtc tcggcgtcct cgacgtcgag gcggtccacg atggcctgca gcgacgccat
     4021 cccgcggaac actttgaacg gcaccttgac cgttttgttc gattcgggga tcacgaccgg
     4081 cttttgaacc gtgtccccgg gggtttccag cgttcccgcg aacacgcttc cgagcatcac
     4141 ggtgtcgccg ccgaagagca gcgcctgcac gatcgagccg tcccgtctga tgccgccgtc
     4201 ggcgatgagc ggcacgcgtc cgcccgcagc agcgcggccg gcggcgagcg cctccacctg
     4261 cggcacgccg aagttcgtgt tcagccgggt cgtgcagccg ccgccggggc cgatgccgac
     4321 cttgacagca ttggcgccgc gctcgacgag aaagcgcacg ccgtcggcgg tcgcgacgtt
     4381 gccggccacg agctctgcat cgccgaagcg cgagcggaag gcgtcgatcg cgcgcgccat
     4441 gatcgcggaa tggccgtggg cgatatcgat gacgatcagg tccgcgccgg cgcgaagcag
     4501 ctcggtggcg cgctcgaggt aatcgccctt ggcgccgatc gcggcgccca cccgcagccg
     4561 cccatgggcg tcacgcgtgg caaagggaag ctgctcctgc ttgacgaggt ccttggccgt
     4621 gaccaggcca atcagtcgaa ggtcgccgtc gacgagcggc agcttcttga ccttgtgttc
     4681 gcgcatcact gcctcggcgg cgggagcgtc gatggccccc tggcgcacga cgaggcgatc
     4741 gcgcggcgtc atccgggccg ccacggacgc ggaagggtcg gcgaacctca tgtcccgcgt
     4801 ggtcagcagg cccagcacgc gccgagcctc gtcgacgacc acgagagtcc cgacgccagt
     4861 ccgctgcatc acggcggagg cgtcggtcag cggcgcctcc gggccgatcg tgtacgggtc
     4921 gccgatgatc ccgtgctgac gccgcttcac gcgctccacc tcgcgcacct gcggctcgat
     4981 gtcgccggcc cggaagccgc ggtcgatgat gccgatgccg ccttcctcgg cgatggcgat
     5041 ggccatttcg gcccgggtga ttgtgtccat gttggccgag acgatcggcc gtttcagggt
     5101 caggtgctcg gacagccggc acgagagatc gatggtggac gggtcgcgcc gctcgatgac
     5161 gccgtactgc ggcgtgaaga gaaagtcgtc gaacgtgcag ccgcgaacca ggtcgagcaa
     5221 gtcggacatt ggaccaatcc aaggtttcgg acgttcagcc accggccacc agctaccata
     5281 ggcccgtgtg ggtcctgctt ctgggagtgg cggccacggc gtgggtcgcg ctcctcgtcg
     5341 ttgcgccgta cttgccagca tatgccggcg cggtgctcta cggcaccggc tcgctcgtct
     5401 gccaccaaat ctccgagcgc tcttttcacg tggccggctc tcagctgccg gtctgcgcga
     5461 gatgtctggg cttgtacgcc ggagcatggc ttggagtgat cggccgtctc tggtggggcc
     5521 gcgtcccggc gttggacagt tcgcgcacgg ctcgcgccgc cgccgtcctg gccgcagttc
     5581 ccaccgccgt gacgtggacg ttcgaggtgg tcggcgtctg gaacccttcc aacgcgacgc
     5641 gtgccatcgc gggactgcct ctcggcgctg tcgtcggtct tgtagtggcg ggcgcggtcg
     5701 ctacgctaca ctacgatgaa tgcgtgccgc cgcggccgac cgcgcccagg ccgcctccac
     5761 cgtctatctg atctgcgcgg ctgcctggct cctgccgggc gccgggcatc tgtggctggg
     5821 gcggcgtcag aaggggctgg tgttcctcgt cgctctgacg ctgatgttcg ggttcggcct
     5881 gtggctggag ggccgtctct tccccttcga ggtcacgcag ccgctgatcg ttctcgccgc
     5941 catcgcggac ctggggatgg gcgcgccgta tcttgccgcg aaagcgttcg gcgcgggcgc
     6001 cggccgcgta gtcgcgatca cctatgagta cggcaacacc ttcatcatcg tcgcgggcct
     6061 gctgaacatg ctcgtggtgc tcgacgtatt cgacatcgcc aaggggcgca agtgacgagt
     6121 cacctcggcc tcatggtgat cttcgccgcc ttcgtgtcgg tcgtcttcgc ggtgctgagc
     6181 cgcgacgagc ccagggacca gatggtgctt ggcgcgcgca tgttcggcgg cttcgtcttc
     6241 gccggcgtgc tcatcggctg gttgctgtac ccgcttccgt tctgagggcc gctgccacga
     6301 ccagcctcgc gcgccgcgta tccctctggg cgccggtcgt cgcgtacatg gcgctcatct
     6361 tcttcgtctc gtcgcagccg agcgcgccgc tgccggagtc ggtatccgac aagggcgctc
     6421 acctgctcgc gtactcggtc ctcggcgtgc tgtcggtccg cgcggccggc ggcggactgc
     6481 cgtgccgggt gacggtgccc atcgcgctga cggcgctcgc catcacgagc gggtatggcg
     6541 cgctcgacga atggcgtcag ggcgtcgtgc ccggtcgaag ccaggacatc gcggactggt
     6601 acgcggacac cgcgggcgcc gtgatcggca tcggcgtgtg ttgggcgtgg ggtatgatgc
     6661 gggcgctggg ccgaagatcg ccgtgaagga gttgcagggc tcgaaacgtg cggccggttg
     6721 tcggttcgcg atcgtcgtat cgcggttcaa cgccgagatc accgagggcc tgttgcaggg
     6781 cgcgtgctcc gcgctcgccg acgccggagt cgggagcgaa gatgtcacca tcgtccgcgt
     6841 accaggggcg ttcgagatcc cgctcgtggc gcggcgcctg gcgaagacgg ggcgcttcga
     6901 cgcggtggtc tgcctgggat gcctgatcaa aggcgagacg atgcacttcg aatacattgc
     6961 gtccgcggtc tcgcagggga tcatggatgc cggcgccgac accggcgtac cggtgacctt
     7021 cggcgtgctc acggcgttga ccgacgaaca agcggcctcc cgcgccttgg gcggcgccga
     7081 caacaagggc cgagaggcgg cgatggcggc cgtggaaatg gcgaccctcg gccgccggct
     7141 gaacgaagag ggccaacgat gaaggccggc gctgtgcccc ggccggccgt ccggcagcgt
     7201 gccgagtcgc ggcaccgcgc gcgcgaaacg gcgctgcaga tgctctatca gtgggaggtc
     7261 ggacggacgc cgatcgagga ggtctgccgg acgttctggt cggatggtcc ttccacggtg
     7321 cggctcggcg acgcgcttcg cgccttcgcc gtcgcgctcg cgactggagt cgccacacat
     7381 acgaccgggc tcgacacgct catcgttgac gccgccgagc attggagaat cgagcggatg
     7441 aacgtcgtcg atcggttgat cctgcggctt gcggtgtacg agctgctgta cgagccggat
     7501 acgccgggca aggtcgtcat caacgaggct ctcgagttgg ccagaacctt cagcgcgggc
     7561 gaatcaactc gcttcatcaa cggcatgctc gacgccatcc gccggacgct cggacgcgaa
     7621 tgagcgctga agaatcagaa cagggcggcc agcgccgcgc caacttcgac gagctgaaga
     7681 agctcggggt ggatccctac ccgcggcgct tcgagggcgg agtgtcgatc ggcagcctcg
     7741 tcgccgagca tggcgccagg agcggcgagg agctcgagtc gctgcagatc cggaccagaa
     7801 cggcgggccg gatacttgcc atccggagct tcggaaaggc caacttcctt cagctgtccg
     7861 atgggcacgc gcgcgtccag gtgtacgtcc gccaggattc ggtgcccgag ctcgacttca
     7921 aggtgttcaa gctcctcgac atcggcgact gggtcggcgt cgaaggacgg ctctttcgca
     7981 ccaagacgaa cgagttcagt gtctacgcgt cgagcatgca gttcctgtcg aagtgcctgc
     8041 tgccattgcc cgagaagtgg cacgggctgc aggacgtcga aatccgctac cggcagcggt
     8101 acctggactt gatcgtgaac cccgattcac gacgcgtgtt cgaggtgcgc agccgcctcg
     8161 tcacctccat ccgggagttc ctcaccaggc gcgggtttct cgaggtcgag acgcccatga
     8221 tgcagccgat agcgggcggg gcgctggccc ggccgttcgt gacccatcac aacacgctcg
     8281 acatgcgcct ctacatgcgg gtcgccccgg agctctacct caagcggctc accgtcggcg
     8341 gcatcgagaa ggtgttcgag atcaatcgga acttcaggaa cgaggggatc tcgacccagc
     8401 acaatcccga gttcacgatg ctcgagttct actggaccta cgcggactac aacgacctga
     8461 tgacgctgac cgaagacatg ctgcagcagg tcgcgagggc cgccaccggg gccgacgaag
     8521 caacgtttgg cgagcacgtg atctcgttca ggcctccttt caggagactc tcccttcgtg
     8581 agggtgcgcg cgcggcggcg tctgcccgcc ttggaaccgg cgtcacggat cgggagctgc
     8641 ggacccgtga aagcgcggcc gcgctggcgt cccgactcgg catcgaggtc gcgccgggca
     8701 gcggacccgg aaagatcgcg gccgccatct tcgaggcgct ctgcgaagcc gatctcgtgc
     8761 agccggtatt cgtgtacgac tttccaaccg aggtctcgcc gctgtccaag cagaagagcg
     8821 acgaccccga tacggtcgag cgctttgagt tgtacgtcgc gggcttcgag attgccaacg
     8881 ccttcagcga gctcaacgac ccagtcgagc agcggcggcg cttcgagcat cagctcagcg
     8941 ggcgcggcga caggcacgaa gaggcgcacg ccatggacga agactacata cgggcgctcg
     9001 agtacgggct gcccccgacg ggcggcgagg gaatcggtat agaccgcctc gtgatggtgc
     9061 tgaccaacag cccgtcgatc cgggacgtga tcctgtttcc gctgatgcgg ccgcgcgacg
     9121 ggggctagcc ggtccccggt gcctgtgcct tcgtgcctgg tccggctatg gcgacgcgat
     9181 tttgatcgtg acgaggtgct cggagccgat cggctggccg ttcagcgagg ccggcgtgta
     9241 ctgccagtcg cgcacggccc cgaggacgat ctggtcgtac accggctgag aagactgttt
     9301 gacggtcgcc gacgtgactt tcccctcggt atcgatttcc acgacgagcg agagtactgg
     9361 accgacccgc ccctgcaggg ccgcggggat aggggggatt cgctgccgga tgggcgcggc
     9421 tggaatcacc ttcaccggcc tgggcgcgac ggcagcttcc ctgaccgttt ccgcaggcgg
     9481 agcggcaggt ttgctcgcga cctgagccgc ctgggccgcg gacgccgcgc gagcgagatc
     9541 gatgaacccc tgcgcgagcg ttttcagatc ctgcgcgtct gtcgcgttca cgaacgcggg
     9601 gtcggccacg aggttcaaga ccagggagaa ctgcctttcc gcctcctcgt tccgcttggc
     9661 ggcgaaatgc tcacggccgt cggcgaaggc gcggcgggcg atcgatggca gcatcttcct
     9721 gcgggtctcg tccaccagct gcaccagccg cggcggcgca tcgtcggccg acgccacgaa
     9781 cagcggcgac gccgtgacga gcgccgagac cgccgccgcc gcttcctggt tgcgtccgag
     9841 cgcaagcagg cagagcgcct tgtagcgatg agcgtcaggc gtgtcgagcg acgcgagcag
     9901 gacgagcgcc tgatcgtagt cagctgactc gtagagcgtc ttgaagtccg ccggacgctc
     9961 ctgggcgccg agggcggcgg agagcgagat ggagaggatc gttccagccc acgcgcgccg
    10021 ccgtccgcac gcgagggagg gtgtcaccgc gatctcatgt cgacagagat ccgaggcgac
    10081 tctttgagcg tcactcggac cgcctgtcgc ttctcgccaa gctgcggatg gcggaacacg
    10141 atcacgtgat cgccgggtgg cagcgtcacg ttcgccaacg gggtgtcgcc gatgcgcgtg
    10201 ttgttgatga atacctccgc ccacggaatg gcgttgatgt tcatcggcac ctccggcatg
    10261 ttgacggaga ccggcgcggc ctggccggct ctcacgctca cacggctcgc cgagcggagc
    10321 ccgaacggct ccgagacgaa ttcgaggtcg tggttgcctg gcggcagcat gatccggtcg
    10381 atctcgctcg tcccgatcag gcgcccgccc tcgaggacct gaatcacgaa cgacgacttc
    10441 agcgagatcc agccccagga tgtcgagggg gccgtgccgc cgatcacgag cgacgcggtg
    10501 gcgcccggct ccacctgcac ggtgcgccgg tacgtggtgc ccgcggtgcg gacgacgacc
    10561 tcatggcggc ccgcggcgag attgcgcacg gtgagcggcg ttcgtcctcg ctcgacgccg
    10621 tccacgctca cgacgctgtc aggggaatcg ctggtcacct gaagaccgcc cgctgtcgcg
    10681 ggtacgaccg ggggcgcgac ctcggtaaag gagatgtgga agtcccggcc gcctcccttg
    10741 gcgatctcga ccggaagctg cttgacgttc gatccctgcc ggacggccag tgtgtaggcg
    10801 ccgaccggaa gcgtcaggga cagcggcgtc gtgcctcgga cctcgccgtt gaggcgcact
    10861 tcggcgcccg gagggtccga ctcgacgcgg agcgatccgc tcgcggctcc cgcgacgccg
    10921 atcgagtcgg gccaggccca gacggcgcca gcggcggcag ccgtcacggt gacgaccgcg
    10981 agcgtcacgg cgatgatcca ctttcgagcg ctggccgcct gagggacctc ggggacgggc
    11041 ttgctttcgg ccgcaaattc aagcgtcgcg tcttcgtcac cctgggccgg cggcacggcc
    11101 gagcggtcag gaaacgtggc gacggcgtcg gttctgaggg gagtctgggc cacggcgcaa
    11161 ggcacaagca tagctcgttg tgtgccagat tcgccctccg ggattccccc gatccgggca
    11221 atggaaaccg cctaaagtcc gccattaggc cgactgttgt ctgtgtcgtc tgggtgtgtc
    11281 tggcaggagc acggcatccc cgttcctgat ataagtggtg gccagcgtgt cgtttgagct
    11341 ctttgttgcg ctccggtacc tgctggccag ccggcagcac tcatcaatat cccgcgtttc
    11401 cctcttctcg acgatcggcg tcgccgtggg cgtgacggca ctggtggtcg cgctcgcgct
    11461 gatgacaggc ctccaaggcg agcttcgaag ccggattctc ggctcgaccg cgcacgtgta
    11521 cgtctggaag gccggcggcc tggacgatta tcaggcggac gtcgcccggc tgcgagccct
    11581 gccgggcgtg gcaggcgccg gtccggccat cgtggacaag gcgatcgtga ggtcggacag
    11641 ggagtccgcc ttcgtcactc tcaagggcat cgatcccgtg ctcgagtcgc aggtcaccga
    11701 catcgatcga accatcaagc aggggagcgt tgccgcgctc agcaacctgg gcgaggagga
    11761 tcgtcccggc gtactgctcg ggatcgatct cgcgcagcac ctggaagtca aggtcggcga
    11821 ttacgtgtca ctgctgacgc tggccggtat cggatcgccg atgggacgga tgccgtttcc
    11881 gcggcgcctt cgcgtcgcgg gcatctatca gctcggcctg tacgagtacg actcggcgta
    11941 cggcttcgtc acgatagacg tcgccaagcg gctcttcgcc aagacgcaac ccgatctgat
    12001 acagcttcgc gtggacgaca tctggagcgc cgctgcgatt gcgaaatcgg tccccgagcg
    12061 gctctccacc gattacatcg cggaagactg gtccgag
//
LOCUS       DBNGSH010000947         2202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4201954, whole genome shotgun sequence.
ACCESSION   DBNGSH010000947 DBNGSH010000000
VERSION     DBNGSH010000947.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4201954"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="AC16CY_21695"
     CDS             complement(<1..626)
                     /locus_tag="AC16CY_21695"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HIR3356480.1"
                     /translation="MITLTLQEQPNVPLEAESLSPDVMAPLSHDAIRALPVYLGKRQK
                     RVDDFFTVEGEGSEELVIDGDATKIKWIGRGMTRGRITINGHAGMHLGAYMTGGVIEV
                     SGNTTDWLGAEMSGGLIRIRGDAGGQVGAAYRGSLSGMTDGTILIDGSAGLEVGMRMK
                     RGIIAVGGAVRDFAGLQMKGGTIVLGGGAELRTGAWMIRGTIVSLKPIP"
     gene            complement(623..>2202)
                     /locus_tag="AC16CY_21700"
     CDS             complement(623..>2202)
                     /locus_tag="AC16CY_21700"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HIR3356481.1"
                     /translation="DGVVKDVCIAGGRIVDRVEGGRTIDASGTIVFPGGVDAHTHVAG
                     GALNFARGLVPENQRVARKFLHTTKYRAGIGGATPTTFATGYLYAGMGWTTVNEAAVP
                     ILTAKHTHEELHDTPIVDKSCLLLMANNEIVLDLLEAGEVERARHVVAWLIWAAKVYG
                     VKAVNPGGVAAWKWGKNASKLHEPVEGYAKTTPAHIIAGLAQIIDDLGLPHPLHLHCN
                     NLGNPGNIVTTLETMKVLEGRRAHLAHLQFHSYGGDDWASMRSGAAEIADEFNRQTNL
                     TADAGAILFGDAVTITADGPWQHMLHQLTGRKWGNLDVENETGCGIVPYVYRDRHVVN
                     AIQWAVGLELLLLITDPWRVFLTTDHPNGGCFWRYPEIIRLLMDVEFRKEQVKRLPEV
                     ARGRIVLGDLDRQYTLSEVAIVTSAGPARALGLTQKGHLGVGADADVTVYPASPEDGM
                     LFSYPRYVLKGGEVVVEEGELRGLTEGREFVFRPAYDAQIEEYLRPLFQKVYTISFEN
                     YPVEMERLRHVEVTERR"
BASE COUNT          344 a          770 c          689 g          399 t
ORIGIN      
        1 ggaatcggct tcagagagac gatcgtcccc cggatcatcc aggccccggt ccggagctcg
       61 gcgccgccgc caaggacgat ggttccgccc ttcatctgca gcccggcgaa gtcccgcacc
      121 gcgcctccga cggcgatgat tccgcgcttc atccgcatgc cgacttcgag gccggcggat
      181 ccgtcgatga ggattgtccc gtccgtcatc cccgacaagc ttccccggta ggccgccccg
      241 acctgtccgc ccgcatcgcc gcggatgcgg atgaggcctc ccgacatctc ggccccgagc
      301 cagtcggtcg tgttgcccga cacttcgatg acgccccccg tcatgtacgc accgagatgc
      361 atgccggcgt ggccgttgat cgtgattcga ccgcgcgtca tgccgcggcc gatccatttg
      421 atcttcgtgg cgtccccgtc gatgaccagt tcctcgctgc cctcgccctc gacggtgaag
      481 aaatcgtcaa cgcgcttctg gcgctttccg aggtagaccg gcagcgcgcg gatcgcgtcg
      541 tgcgacagcg gcgccatcac gtccggcgac aacgactcgg cttcgagcgg aacgttgggc
      601 tgctcttgga gggtcagcgt gatcatcgtc tctcggtcac ttccacgtga cgaagtctct
      661 ccatctccac cggatagttc tcgaacgaga tcgtgtagac cttctgaaac agcggacgca
      721 gatattcttc gatctgcgcg tcgtaagcgg ggcggaacac gaactcgcgg ccttcggtca
      781 acccgcgcag ctcgccttct tcgaccacga cctcgccccc tttgagcacg tagcgagggt
      841 acgagaacag cattccgtcc tccggcgaag cgggatagac cgtcacgtcc gcgtcggcgc
      901 ccacgccgag gtgccccttc tgcgtgaggc cgagcgcgcg cgccgggccg gcggaggtga
      961 cgatcgccac ctccgacagc gtgtactggc ggtcgagatc gccgagcacg attcggccgc
     1021 gagccacttc cggaagccgc ttgacctgct ccttccggaa ctccacgtcc atcagcaggc
     1081 ggatgatctc cgggtaccgc cagaaacagc cgccgttcgg atgatccgtc gtcaggaaga
     1141 cgcgccaggg atccgtgatg agcagcagca actcgagccc gaccgcccat tgaatcgcgt
     1201 tgacgacgtg ccgatcgcgg tagacgtagg gaacgatccc gcacccggtc tcgttctcga
     1261 cgtccaggtt gccccacttg cgccccgtga gctgatggag catgtgctgc cacgggccgt
     1321 cggccgtgat cgtgacggcg tccccgaaca ggatcgcgcc ggcgtctgcg gtcaggttcg
     1381 tctgacggtt gaactcgtcg gcgatctcgg ccgctcctga acgcatcgac gcccagtcgt
     1441 ccccgccgta tgaatggaac tgcaggtgcg ccaggtgcgc gcgccgcccc tcgagcacct
     1501 tcatcgtctc gagcgtcgtc acgatgttcc ccgggttgcc gagattgttg cagtgaaggt
     1561 gcagaggatg cggcagcccg aggtcgtcga tgatctgcgc aaggcctgca atgatgtggg
     1621 ccggcgtcgt cttcgcgtag ccctcgacag gctcgtggag cttgctggcg ttcttgcccc
     1681 atttccacgc ggcgaccccg ccgggattga cggccttcac gccatacacc ttcgccgccc
     1741 agatcagcca cgccaccacg tgccgcgcac gctccacctc gccggcctcg agaaggtcca
     1801 ggacgatctc gttgttggcc atgagcagca ggcacgactt gtccacgatc ggcgtgtcat
     1861 gcagctcttc atgcgtgtgc ttggccgtga ggatcggcac cgcggcctcg ttgaccgtcg
     1921 tccagcccat gccggcgtag aggtatccgg tggcaaaggt cgtgggcgtg gcgcctccga
     1981 tgcccgcgcg gtacttcgtc gtgtgcagga acttccgggc cacgcgctgg ttctcgggca
     2041 cgaggcccct ggcaaagttc agggccccgc cggccacgtg tgtatgcgcg tccactccgc
     2101 cgggaaagac gatcgtgccg ctcgcgtcga tggtccggcc tccctcgacg cggtcgacga
     2161 tgcggccgcc ggcgatgcag acgtccttga cgacgccgtc ga
//
LOCUS       DBNGSH010000948         1775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3861944, whole genome shotgun sequence.
ACCESSION   DBNGSH010000948 DBNGSH010000000
VERSION     DBNGSH010000948.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3861944"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="AC16CY_21705"
     CDS             <1..317
                     /locus_tag="AC16CY_21705"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3356482.1"
                     /translation="VELPERPGAREAAYRFGSLDYPATERRFNSYYAEFLPMAPEKLR
                     PHDHAGVEFIYVINGILSVHMNGEEHALEAGDAIYFDATTPHAYRRSGGRLCSAVIVT
                     AS"
     gene            439..>1775
                     /locus_tag="AC16CY_21710"
     CDS             439..>1775
                     /locus_tag="AC16CY_21710"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3356483.1"
                     /translation="MTQGQLQHLTTALAGRYRVLREIGRGGMATVYLADDPKHHRQVA
                     IKVFDAGLAEALGKDRFLREIEVTAGLTHPHILPLYDSGLADGLAYYVTPFVEGKTLR
                     EYILERGRLPLEEALRIVKGVADALAFAHRKGIVHRDIKPENVLLQAGHALVADFGIA
                     RALSLAGDLALTQTGMVVGTPSYMSPEQTVGDSTVDGRSDIYSLACLLFEALTGSPPF
                     RGGTAADVARKRLTEAAPRLRASASDVPAAIDEAVAKALAREPGDRFSTADAFAAALS
                     PSAATPGAARDGAAAAAETKGLVVLPFTNLSPDRENEFFADGLTEEVIADLSGISALR
                     VISRTSAMRFKGTDKDLRAIARELSVRYVLEGSVRRAGSSLRVTAQLVEADTDSHVWA
                     EKYSGSIDDVFAIQEEISRKIVKALQVKLSDTESRQVAERPIDNAVAYDCYLRA"
BASE COUNT          302 a          574 c          605 g          294 t
ORIGIN      
        1 gcgtggaatt gccggagcgc cctggcgcgc gagaggcggc gtatcggttc gggtcgctcg
       61 attacccggc gaccgagcgc cgtttcaaca gctattacgc cgagttcctg ccgatggcgc
      121 ccgagaaact acggccccac gaccatgcgg gcgtcgagtt catctacgtg atcaatggca
      181 tcttgagcgt gcatatgaac ggcgaggagc atgcgctcga agcgggcgac gccatctatt
      241 tcgatgcaac cacgccgcac gcgtaccgac gcagcggcgg gcgcctctgc agcgccgtca
      301 tcgtcactgc ttcatagcgg cgacgccgag acaggcgcgt gaacagattg aaggcgggag
      361 acggcgtgtc ccgatcagct cggcaggccc gattgagtat gatgcgttgt tccagcagac
      421 gaggttggac caggtttcat gacgcaggga cagctgcagc atctgacgac ggctctggcc
      481 ggccgctatc gcgtgctccg cgagatcggc cgcggcggga tggccaccgt gtatctggcg
      541 gacgacccga aacaccatcg ccaggtcgcc atcaaggtct tcgacgcagg cctggccgag
      601 gcccttggca aggatcgatt tctccgcgag atcgaggtca cggccggcct gacgcatccg
      661 catatcctgc cgctctacga ttcgggtctg gccgacggtt tggcctatta cgtgacgccc
      721 ttcgtggagg gaaagacgct tcgcgaatac atcttggaga gggggcgcct tcccctggaa
      781 gaggcgctgc gcatcgtgaa gggtgtggcc gacgcgctcg ccttcgcaca tcgcaagggc
      841 atcgtccatc gcgacatcaa gcccgagaac gtgctgctgc aggcgggaca tgcgctcgtc
      901 gccgacttcg gcatcgcgcg cgcgctcagt ctcgcgggcg acctggcgtt gacgcagacc
      961 ggcatggtcg taggtacgcc ttcctacatg agcccggagc agacggtcgg cgacagcacg
     1021 gttgacggcc gcagtgacat ctacagcctg gcctgtcttc tgttcgaggc cttgacggga
     1081 tcgccgccgt tcagaggcgg tacggcagcg gacgtcgcac ggaaacgtct caccgaggcg
     1141 gcaccgcggt tgcgcgcgtc ggcctccgac gttccagccg cgatcgacga ggcggtggcg
     1201 aaggcgctgg cgcgcgagcc gggtgaccgc ttttccacgg ccgacgcctt tgccgcagcg
     1261 ctgagtcctt cggccgctac gccgggcgcc gcccgtgacg gcgcggctgc cgccgccgag
     1321 acgaaggggc tcgtcgttct cccgttcacg aacctgagcc cggatcgcga gaacgagttc
     1381 ttcgccgacg gcctgaccga agaagtcatt gcggacctgt cgggcatcag cgcactgcgc
     1441 gtgatctcgc ggacctccgc gatgcgcttc aagggcaccg acaaggatct ccgggcgatc
     1501 gcgcgcgagc tgagcgtccg ctacgtgctc gagggcagcg tgcggcgcgc gggatcgagc
     1561 ctgcgcgtca cggcgcagct ggtcgaggcc gacaccgatt cgcacgtgtg ggccgagaaa
     1621 tattccggca gcatcgacga cgtgttcgcg atccaggagg agatctcgcg gaagatcgtc
     1681 aaggcgctgc aggtcaagct gtcggatacc gagtcgcgcc aggtggccga gcgtcccatc
     1741 gacaacgccg tcgcctacga ctgctacctg cgggc
//
LOCUS       DBNGSH010000949         6709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_977372, whole genome shotgun sequence.
ACCESSION   DBNGSH010000949 DBNGSH010000000
VERSION     DBNGSH010000949.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_977372"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="AC16CY_21715"
     CDS             complement(<1..214)
                     /locus_tag="AC16CY_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3356484.1"
                     /translation="MLSLAILVSAAAANAQDLVLTNATIVDPASRTTVRGALWIEGGK
                     IVGRGADPPAGARGERIDLQGRWVIPG"
     gene            complement(365..799)
                     /locus_tag="AC16CY_21720"
     CDS             complement(365..799)
                     /locus_tag="AC16CY_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356485.1"
                     /translation="MATYTYQGKPDLVDVPDPAIPPIPRLMAMAISSAFCPVKERCVE
                     GEEIVFNLPPGTPSEVYQRVIGHLGTARPIRHSWERAVHVMEVRARAVESEVEVIVGS
                     PGIPAYGTGVEYTVSILNGQRVAALRVGRTNVTPPAPHCPEC"
     gene            complement(889..2919)
                     /locus_tag="AC16CY_21725"
     CDS             complement(889..2919)
                     /locus_tag="AC16CY_21725"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3356486.1"
                     /translation="MAVGSLSVAFGLAQTKQGSGDDFSAARLTAAPTTSWPTNGGNLY
                     NQRYSPLTSINRANVGQLKGVWRARLGGSGTAPQYSGFAQPIVYDGVAYVSTGANDVF
                     ALSIDTGAILWQYSAHLDPNITSVCCGWNNKGVAVSADRVFIGQLDDRLVALDRATGK
                     VVWSIQAERWQDAFSITAAPLYINGTVIIGFAGGDRGTRSRLKAYDAKDGRLIWTFYT
                     IPGPGEPGHETWPKDNDAWKYGGAAIWQTPAVDPELGLVYFSTGNAAPDYNGAFRVGD
                     NLYAASMLAIELRTGKYRWHFQQVHHDIWDYDAVNPVVLFDASIGGRTRKAIAEVGKT
                     GWAYILDRETGKPLIGIDEKPVPQEPRQATARTQPFPRGDAIVPQLIEIAPEGHALVN
                     DGRIFTPFVGTNSTIVVPGIWGGASWPPSSYDPAQQRLFVCASSVINGFTGGGDPKFA
                     APTSGASYLGGATTFTRAARTGIIAALDVTTNRLAWRYQWPEQCYSGTLATAGGLLFV
                     GRNDGRLTALDSSTGKQLWEFQTGAGMHAPASTFDHRGKQYVLAFSGGSALIGSARGD
                     SVWLFALDGTLSPERPGTPVSRQAAAATTPLNLAEGRRLFAQACAVCHGDDGKGGHTG
                     GAPLDRVADLAAAVQTVTEGRNTMPAFRTAFTPEQIRDVTAYVVETLVRSPR"
     gene            3046..3630
                     /locus_tag="AC16CY_21730"
     CDS             3046..3630
                     /locus_tag="AC16CY_21730"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3356487.1"
                     /translation="MDSYTLPHRHAARWSGAALVAAAILCASPQGAAQQKSSGNDPVA
                     RGRYLVGITGCHDCHSPKIKGMTPNLDLALSGRPASTPLPSPAKDEVHASADLTAWQG
                     PWGYSVASNLTPDEATGIGKRYTEASFIATMRTGKKPNGTPVMPPMPAEVYQNMTDED
                     LRAIWAYLRTVKPIRNAVLAGLPPPAAQKPAGQK"
     gene            complement(3641..3985)
                     /locus_tag="AC16CY_21735"
     CDS             complement(3641..3985)
                     /locus_tag="AC16CY_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356488.1"
                     /translation="MKPEELDRVLSGEADIVPSSGFVASVMEAVTAEASAAPLAFPWK
                     RAWPLAAGFPVLFLWLAVSQIGIGPEAATPGPDLYEWFERIVPMATGWVASGLLMTAA
                     ITVWSLRLIRLR"
     gene            complement(3982..4533)
                     /locus_tag="AC16CY_21740"
     CDS             complement(3982..4533)
                     /locus_tag="AC16CY_21740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3356489.1"
                     /translation="MSAEEDAADVNRVLAGDISAFEPIVRRWQAPLVNLAYRFCHDRS
                     RAEDMAQEAFLRAYRNLARWRREAAFSTWLFAIATNLYRSEIRRLPPLTVPIDEAAAT
                     PDPRAAEPPLDDERRDRLVRSAVLALPAKYRDVLTLFYFHGKDVALSAASLGVPQGTV
                     KARLSRGRDILRSKLPRLLRESV"
     gene            4670..5065
                     /locus_tag="AC16CY_21745"
     CDS             4670..5065
                     /locus_tag="AC16CY_21745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356490.1"
                     /translation="MESGLWIFLSFTVVSVFSFVAVAVWAGTRHEERKEFYRSETLKK
                     LAESGSAAVIEYLREEEKQEERRRAERHEQMIQGNRLGGLILLVVGALVAVALRQLVP
                     GLPVYLFGMIPFGIGVVLLLTPFITRRRS"
     gene            complement(5073..5855)
                     /locus_tag="AC16CY_21750"
     CDS             complement(5073..5855)
                     /locus_tag="AC16CY_21750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3356491.1"
                     /translation="MTVATSTLLGRPLWYELMTSDMKAAEAFYQTVVGWKTSPFDGAG
                     QPYSMFNRSGDVPVGGVMTTPSEVKAPPFWAMYIGVPRLEDAAAHIKTLGGRECTEVI
                     TVPSIGRMQMMADPQGAAFYLYEPASEDQPPEGAAEVGEASWRELMTTDAPAAMKFYE
                     QVFGWQPTQTMDMGPAGMYQVFNRPHGMIGGMMNRPPEMANVPPNWQMYFRVPDVHAA
                     AERVKANGGQIVNGPMELPDGDWIANAIDPQGAAFGLHAKKA"
     gene            complement(6019..6480)
                     /locus_tag="AC16CY_21755"
     CDS             complement(6019..6480)
                     /locus_tag="AC16CY_21755"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3356492.1"
                     /translation="MAVCLAALAVVFEATALGHHSFAMYDQKKVVTLTGVVTQFVPQA
                     NHAEIHFVLLTPDHKGPAKGPDGKYVQWGIEMAGAAQLDRQGINAKTYAPGTVISVHL
                     NPLRDGSNFGARTGVMARCPIDPATNKSKLPEPGKHCDSVPGHTLSGGTAF"
BASE COUNT         1093 a         2252 c         2237 g         1127 t
ORIGIN      
        1 gcccgggtat gacccagcgc ccctggagat cgatccgctc gccgcgggcg ccggccggcg
       61 gatccgcgcc gcggccaacg atcttgccgc cttcgatcca gagcgcgcca cgaaccgtcg
      121 tcctcgaggc gggatcgacg atggtggcgt tggtgagtac gagatcctgg gcattggcgg
      181 ctgccgccga gaccaggatt gcgagagaga gcaatccgtg tcgcataact ccctttgacg
      241 gcaactcatg acaccgcgtt tgctccggtc gcccgcgtcc ggccaaagct gcccttcgac
      301 acggcgcgag ggccttctga taggcgtttt gaaccgtacc ggcgtcacgc cttgattccg
      361 ctggtcagca ctcggggcag tgcggtgccg gcggtgtcac gtttgtgcgc cctacccgaa
      421 gagctgcgac gcgctgtccg ttgaggatgg agaccgtgta ctccaccccg gtgccatatg
      481 ccgggatgcc gggggaaccg acgatgacct cgacctccga ctcgacagcc cgggcgcgta
      541 cttccattac gtgtaccgcc cgctcccacg aatggcgaat cggcctggcc gtgccgaggt
      601 gtccgatcac gcgctggtac acctcggacg gcgtgccggg gggcaggttg aagacgatct
      661 cttctccctc gacgcagcgc tctttgacgg ggcaaaacgc ggaactgatc gccattgcca
      721 tgagcctggg gatcggcggg atcgcggggt caggcacatc gaccaagtcc ggcttgccct
      781 ggtacgtgta agtggccacg ggagtacagg cacccgcggc gagcaagacc gggagagcaa
      841 cgacccagag cgccttacag agcggccgcg gcatcggtgg ccatcgcttc aacgaggcga
      901 tcttaccagg gtctccacca cgtaggcggt cacgtcgcga atctgctcgg gcgtgaacgc
      961 cgtccggaag gccggcatgg tgttgcggcc ttcggtgacg gtctgaaccg cggccgcgag
     1021 atccgcgacg cggtcgagcg gcgcgccgcc cgtgtggccc cctttcccgt cgtcgccatg
     1081 gcagaccgcg cacgcctgcg cgaacaggcg ccggccttcg gccaggttca acggcgttgt
     1141 cgcggcggcg gcctgccgcg acacgggcgt gcccggccgt tcgggagaaa gcgtgccgtc
     1201 gagagcgaac agccacacgc tgtcgccgcg cgcggagccg atcagcgcgc tgccgcccga
     1261 gaacgcgagc acgtactgct tgcccctgtg gtcgaacgtg ctcgcgggtg cgtgcatgcc
     1321 ggctccggtc tggaattccc agagctgctt ccccgtcgag gagtcgagcg cggtgagtcg
     1381 gccgtcgttg cggccgacga acagcagccc tccagcggtc gcaagcgtgc cgctgtagca
     1441 ctgttcgggc cactggtatc gccatgcgag cctgttcgtc gtcacatcca gcgccgcaat
     1501 gatgcccgtc cgcgccgcgc gcgtgaacgt cgtcgcgccg ccaagatacg aggcgcccga
     1561 ggtgggagcc gcgaacttcg gatcgccgcc gccggtgaag ccgttgatca ccgatgaggc
     1621 acagacgaac aagcgctgct gcgcgggatc gtaagagctc ggcggccagc tggctccacc
     1681 ccagatgccg ggcaccacga tcgtcgagtt cgtgccgaca aacggcgtga agatgcggcc
     1741 gtcgttgacg agcgcgtgcc cttccggagc gatctcgatc aactgcggga cgatggcgtc
     1801 gccgcgtggg aagggctggg tcctggccgt tgcctgccgc ggctcttgcg gcaccggctt
     1861 ctcgtcgatg ccgatcagcg gcttccccgt ctcgcgatcg aggatgtacg cccacccggt
     1921 cttacccact tcggcgatcg ccttgcgcgt gcggccgccg atgctcgcat cgaaaaggac
     1981 gacgggattg accgcgtcgt agtcccagat gtcgtgatgt acctgctgaa agtgccatcg
     2041 atacttgccg gtcctgagct cgatcgccag catcgaagcg gcgtacaggt tgtcgccgac
     2101 gcggaaggcg ccgttgtagt cgggggccgc attgccggtc gagaagtaga cgagaccgag
     2161 ctcgggatcg accgccggcg tctgccagat cgcggcgccg ccgtacttcc aggcgtcgtt
     2221 gtccttcggc cacgtctcgt ggccaggctc gcctgggccc ggaatcgtgt agaaggtcca
     2281 gatcagacgt ccgtctttcg catcgtaggc cttgagccgg ctgcgcgtgc cgcgatcgcc
     2341 gccggcaaag ccgatgatga cggtgccatt gatgtacagc ggcgcggcgg tgatggagaa
     2401 cgcgtcctgc cagcgctcgg cctggatgga ccagacgacc ttcccggtcg cacggtcgag
     2461 cgccacgagc cggtcgtcga gctggccgat gaagaccctg tcggcactga ccgcaacgcc
     2521 tttgttgttc cacccgcagc agacggacgt gatgttggga tcgagatggg ccgagtactg
     2581 ccagaggatc gcgcccgtgt cgatcgacag cgcgaacacg tcgttggcgc ccgtgctgac
     2641 gtaggcgacg ccgtcgtaca cgatcggctg cgcgaagccc gaatactggg gcgccgtgcc
     2701 ggagccgccg agacgcgcgc gccacacgcc tttgagctgc ccgacattgg cgcggttgat
     2761 cgacgtgagc ggggagtagc gctggttgta caggttgccg ccgttggtcg gccagctggt
     2821 agtgggcgcc gcggtcagcc tggcggctga gaagtcatcg ccgctcccct gtttcgtctg
     2881 cgcgaggccg aacgcgacgg acagcgagcc gactgccaag gcgagcgctc ccaacctgct
     2941 tgacggagtc gtggcggtca caacggggta tagtaccgcg tccgccggcg cggccgtcct
     3001 tttcatacgc gtgatgggcc acgtcggcac agggaggcga ccgggatgga ctcatacact
     3061 cttccgcatc gacacgccgc acgctggtcg ggcgccgcgc tcgtggcagc cgcgatactc
     3121 tgcgcgtctc cgcagggtgc ggcccagcag aagtcttcag gcaacgaccc cgttgcgcgc
     3181 ggccgctacc tggtgggcat caccgggtgc cacgattgcc attccccgaa gatcaagggg
     3241 atgacgccga atctcgacct cgcgctgtcg gggcggccgg ccagcactcc cctgccgtcg
     3301 cccgccaagg acgaagtcca cgcgtcggcg gatctcacgg cgtggcaggg cccctggggg
     3361 tacagcgttg cgtccaacct gacgccggac gaggcgaccg ggatcggaaa gcgctacacc
     3421 gaggcctcgt tcatcgccac gatgcgcacc gggaagaaac ccaacgggac gcccgtgatg
     3481 ccgccgatgc ccgccgaggt ctaccagaac atgacggacg aggacctgcg cgccatctgg
     3541 gcgtatctgc ggacggtcaa gccgatcaga aatgccgtgc tcgcgggact gccgccgccg
     3601 gctgcacaga agccggccgg acagaaataa ggcgatcagg tcaccgcagg cggatcagtc
     3661 gcagcgacca gaccgtgatg gccgcagtca tcaggagtcc cgacgcgacc cagccggtcg
     3721 ccatcggcac gatcctctcg aaccactcgt agagatccgg tcccggggtg gcagcctcgg
     3781 ggccgatgcc gatctgcgac accgccagcc acaggaacaa cacggggaac cccgcggcga
     3841 gcggccacgc ccgtttccac gggaacgcca gcggcgccgc cgacgcctcg gcggttaccg
     3901 cctccatgac ggacgccacg aatcccgacg aggggacgat gtcggcttcc ccggacagca
     3961 cgcgatcgag ctcctcaggc ttcataccga ttcccgcaac agccgcggca gtttactcct
     4021 caggatatcg cgcccgcgcg acagccgcgc cttgaccgtc ccctgcggga cgccgaggct
     4081 tgcggccgac agggcgacgt cctttccatg aaagtagaac agcgtcagca cgtcccgata
     4141 cttcgccggc agcgcgagca ccgccgagcg aaccaggcga tcgcgccgct cgtcgtcgag
     4201 gggcggttcg gccgcgcgcg ggtcgggcgt ggctgcggcc tcgtcgatcg gcaccgtcaa
     4261 cggcggcagc cgccggatct ccgatcggta caggttcgtg gcgatcgcga agagccacgt
     4321 ggagaacgcc gcctcccgac gccagcgcgc caggttccga taggcgcgga gaaacgcttc
     4381 ctgcgccatg tcctccgcgc ggctgcggtc gtggcagaac cggtacgcga gattcaccag
     4441 cggagcctgc caccggcgga cgatcggctc gaaggccgag atatctcccg ccagcaccct
     4501 gttcacgtcc gcggcgtcct cctcggcgct cacgtggctt cctgctgtag tctgacagcc
     4561 gcctcctcgg gcgggttgcg ttatttccaa acgggccttg cggaaacaaa acgcaaccgc
     4621 ccgcgagacc cgcaggtcat acccaacgaa cgtcacggag gtgagcgcca tggaatctgg
     4681 cttgtggatt tttctgagct ttacggtcgt ctcggtgttc tccttcgtgg ccgtcgccgt
     4741 gtgggcgggc accaggcacg aggaacgcaa ggagttctac cgcagcgaga cgctcaagaa
     4801 gctggctgaa tctggctcgg ccgccgtgat cgagtatctg cgcgaagagg aaaagcaaga
     4861 ggagcggcgg cgtgcggaga ggcatgagca gatgatccag gggaaccggc ttggcgggct
     4921 gatcctcctg gtcgtcggcg cactggtggc cgtggcgttg cgtcaactcg tccccggcct
     4981 gcccgtctat ctgttcggca tgatcccctt cggcatcgga gtcgtgctgc tcctcacgcc
     5041 gttcatcacc cgccgccgtt cgtgacgcgt gtctacgcct ttttcgcgtg aagaccgaac
     5101 gcggccccct gcggatcaat cgcgttggcg atccagtcgc cgtcgggaag ctccatcggg
     5161 ccgttgacga tctgcccgcc gttcgccttg acgcgctcgg cggcggcgtg cacgtccggt
     5221 acgcggaagt acatctgcca gttcggcggg acgttcgcca tctccggcgg tctgttcatc
     5281 atgccgccga tcatcccgtg cgggcggttg aagacctggt acatgccggc cggccccatg
     5341 tccatcgtct gggtgggctg ccagccgaac acctgttcgt agaatttcat cgccgccggc
     5401 gcgtccgtcg tcatcagctc gcgccacgac gcttcgccga cctctgccgc gccctccggg
     5461 ggctggtcct cgctggccgg ctcatagagg tagaacgcgg cgccctgcgg atcggccatc
     5521 atctgcatgc ggccgatgga gggtaccgtg atcacttcgg tgcactcgcg gccacccagc
     5581 gtcttgatgt gcgccgccgc gtcctcgagc ctcggcacgc cgatgtacat cgcccagaac
     5641 ggcggcgcct tcacctcgct cggcgtcgtc atcacgccgc cgacggggac gtcgccgctg
     5701 cggttgaaca tcgagtaagg ctggcccgcg ccgtcgaatg gcgacgtctt ccagcccacg
     5761 accgtttggt agaacgcttc ggccgctttc atgtcgctgg tcatcaattc gtaccagagg
     5821 ggccgtccga gcagggtcga tgtggcaaca gtcatagaaa cctcctgccg ttcacgaaca
     5881 cgccgctgac ctcgacagag gagcggacga gtgcccagca aacgtgcagt ctgtcacaca
     5941 ccggtgacgg ccgcacgacg accggctgag gtccgggagg aattacctgg gacgaatggt
     6001 gggacgaggg tcaaactctc agaaggccgt gcccccgctc agcgtatgcc ctgggaccga
     6061 gtcgcagtgc ttgcccggct cggggagctt cgacttgttt gtggccggat caatgggaca
     6121 cctggccatg acgcccgtgc gggcgccgaa gttcgaaccg tcgcgaagcg gattcaggtg
     6181 gacgctgatc acggtgcctg gcgcatacgt cttcgcgttg atgccctggc gatcgagctg
     6241 agcggccccc gccatctcga taccccactg gacgtacttc ccgtcggggc ccttggcggg
     6301 ccctttgtgg tccggggtga gcaggacgaa gtggatctcg gcgtgattgg cctgcgggac
     6361 gaactgcgtg acgactcccg tcagcgtcac gaccttcttc tggtcgtaca tcgcgaacga
     6421 gtggtggccc agggccgtgg cctcgaacac cactgccagc gcggccaggc agactgccat
     6481 cgtcaaggct ctcggtatgc gtgcggtcat ttctgcggcc ccaataacgt gaagataccc
     6541 tatttgaaga tgtcggtcac ggccgacggg agctcgtccg ccggcttgtc ccatccggcc
     6601 tcgaagtact tctcccagtt ctttgcccac gggcggccgt aataatcgac gaagcgatcg
     6661 tcggacgccg tcacctgctt cgggcgcccg tcggtattgt agatgttgc
//
LOCUS       DBNGSH010000950         2663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_466984, whole genome shotgun sequence.
ACCESSION   DBNGSH010000950 DBNGSH010000000
VERSION     DBNGSH010000950.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_466984"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="AC16CY_21760"
     CDS             complement(<1..170)
                     /locus_tag="AC16CY_21760"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3356493.1"
                     /translation="MDDPAAVERGKAAYSTNCAFCHGADTRGGDGGPSLLRSALVLDD
                     QKGELIGPVIRGG"
     gene            complement(337..462)
                     /locus_tag="AC16CY_21765"
     CDS             complement(337..462)
                     /locus_tag="AC16CY_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356494.1"
                     /translation="MRTTVFLVAADAALLPPKGEIVFAGSYQLWSTVLRPYLTAR"
     gene            486..881
                     /locus_tag="AC16CY_21770"
     CDS             486..881
                     /locus_tag="AC16CY_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356495.1"
                     /translation="MRYLVTARLKPGRRPALDRAIQDGSLGAGSIAGDEYLHNMSHAR
                     QLTDGSVKWVETCFCATPLAEERPYWESFFTLVSVKDAHARSRCRHETGDEPWACSDC
                     DCTDRLEARLRETGEPFCKFAVHGSPFKA"
     gene            878..1936
                     /locus_tag="AC16CY_21775"
     CDS             878..1936
                     /locus_tag="AC16CY_21775"
                     /inference="COORDINATES: protein motif:HMM:NF024136.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HIR3356496.1"
                     /translation="MKVWQVLVVALAAGAAVAPLDARLVERWYSAGVYPAFQRLVTPV
                     SNLFPFAWFDLVTIALAALLLTLLVRLVRRAREGEWRLAFTTLVNLVTAVALIYLVFL
                     SAWGLNYRRVPMSERIVLERDRPTPRDVLALGISAVDKLNELHRAAHEAGWRTAPWRD
                     RQMQQAAVAVQGMLSDAAPAVPGRLKWSIYGPFFRWSNVDGMVNPLGLEVIGNPDLLP
                     FERPFVAAHEWAHLAGYAGEAEASFVGWLTCLHAGAPAAYSGWLFLFWQINSEVGAAD
                     RKQMAALLGPGPREDIAAINARLTRGEVPVVRNASWRVYDQFLKANRVEHGIRNYGAV
                     ITLILRARFEENWTPVRR"
     gene            complement(1969..>2663)
                     /locus_tag="AC16CY_21780"
     CDS             complement(1969..>2663)
                     /locus_tag="AC16CY_21780"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF015446.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3356497.1"
                     /translation="ARALIAAAAVPVAAPSANLFSRPSATQASHVIEDLDGRIDLVLD
                     AGPATIGVESTVLDLSRETPIVLRPGAVTIDMLREVLPRVDVRATLSGAAAASAAAPM
                     PSPGMLEKHYSPRATLTLYEGNGKTAVTSLAGAARAAVADGKYVGVIAYDDDLAALQL
                     DGSSGRILVRTIGSHHSAAMVASRLYGAMRELDAAGVDLILVRGFADGRGLGLAVQDR
                     LRRAAAGRIVQC"
BASE COUNT          422 a          875 c          949 g          417 t
ORIGIN      
        1 ccgcctcgga tcacgggccc gatgagctct cctttctgat cgtcgagcac cagcgccgag
       61 cgcagcaggc tcggcccccc atcacctcca cgagtgtcgg cgccgtggca gaaagcgcag
      121 ttggtgctgt aggcagcctt gccgcgctcg accgccgcgg gatcgtccac cgtccgctgc
      181 gggtaggctc cgatgcccgc gccgccgatt cgaccgcggc cgccggggcc tgcgccggtg
      241 gctgctgggc cgccagcacg gcggagagga agaacacggt ggacaggaga aggaaagtgc
      301 gaaggtgcga ggcggacatt attcggcggg ctcgtgtcag cgtgccgtca gatacggacg
      361 cagtaccgtt gaccagagtt gatagcttcc ggcgaaaacg atttcacctt tgggcggcag
      421 caacgccgcg tcggccgcca cgagaaagac ggtcgtgcgc atgcgcgaaa gagtaacatc
      481 aatcaatgcg ctacctggtg accgcccgcc tcaagcccgg caggcgcccg gcgctcgatc
      541 gcgcgattca ggacgggagt ctgggagccg ggtcgatcgc gggcgacgag tacctccaca
      601 acatgtcgca cgcccgccag ttgacggacg ggtcggtcaa gtgggtggaa acctgtttct
      661 gcgccacgcc cctcgcggaa gagcggccct actgggagtc gttcttcacg ctggtctcgg
      721 tgaaggatgc ccatgccagg agccgctgcc gacatgagac cggcgacgag ccctgggcct
      781 gttccgactg cgattgcacg gatcgcctgg aggcacggct ccgggaaacc ggggagccgt
      841 tctgtaagtt cgcagttcac ggttcaccgt tcaaggcttg aaagtctggc aagtcctcgt
      901 cgtggcgttg gcggctggcg ccgcggtcgc gccgctcgac gcgcgcctcg tcgagcgctg
      961 gtactccgct ggcgtgtatc ccgcgtttca gcggctggtc accccggtat ccaacctctt
     1021 tccgttcgcg tggttcgatc tcgtcacgat cgccctcgcg gcgctcctgc tgaccctgct
     1081 cgtgcgcctc gtccggaggg cgcgggaagg ggagtggcgg ctcgcgttca cgacgctcgt
     1141 caatctggtc acggcggtgg cgctgatcta tctcgtgttc ctgtcggcgt ggggcctcaa
     1201 ctacaggcgc gtgccgatga gcgagaggat cgtcctcgag cgggaccggc cgacgccgcg
     1261 cgacgtcctc gctcttggca tctccgcggt cgacaagctg aacgaactcc accgggccgc
     1321 gcacgaggca gggtggcgga ctgcgccctg gcgcgaccgg cagatgcagc aggccgcggt
     1381 ggcggtgcag gggatgctct cggacgccgc ccccgcagta ccaggccgcc tgaagtggtc
     1441 gatctacggt cccttcttcc ggtggtcgaa cgtcgatggc atggtcaacc ctctgggact
     1501 cgaagtgatc ggcaatcccg atctgctgcc gttcgagcgc ccgttcgtcg ccgcgcacga
     1561 atgggcgcac ctggcgggct acgccgggga ggcggaagcg agcttcgtcg gctggctgac
     1621 ctgcctgcac gccggggcac cggcggccta cagcggctgg ctcttcctgt tctggcagat
     1681 caactcggag gtcggcgccg cggatcggaa gcagatggcg gcgttactcg ggccgggccc
     1741 tcgggaggac atcgccgcca tcaacgcgcg gctcacgcga ggagaggtcc cggtcgtgcg
     1801 caacgcgagc tggcgagtgt acgaccagtt cctaaaggcc aatcgcgtcg agcacggaat
     1861 ccgcaactac ggcgccgtga tcacgctgat ccttcgcgcg cgcttcgagg agaactggac
     1921 gccggtcaga cgatagtcag ggggcagggg gcaggggcag gggacagttc aacactgaac
     1981 gatgcggccg gcggccgcac gcctgaggcg gtcctgcacg gcgagaccga ggccccgccc
     2041 gtcggcgaag ccgcggacca gaatcaggtc cacgccggca gcgtccagtt cacgcatcgc
     2101 accgtagagc ctggaggcga ccattgctgc ggagtggtgc gagccgatcg tgcggacgag
     2161 aatgcggccg ctcgagccgt cgagctgcag cgcggcgagg tcgtcgtcgt acgcgatgac
     2221 gccgacgtac ttgccatcgg cgaccgccgc gcgcgccgcc ccggcgagcg atgtcaccgc
     2281 ggttttccca ttgccctcgt acagcgtcag cgttgcgcgc ggtgaatagt gcttctcgag
     2341 catgcccggg gacggcatcg gcgcggcggc cgacgctgcg gctgccccac tgagggttgc
     2401 acgcacgtct acccgcggaa gcacctcgcg gagcatgtca atcgtgacgg cacccgggcg
     2461 aaggacgatc ggcgtctcgc gggagagatc gagcaccgtc gattcgacgc cgatcgtggc
     2521 cggaccggcg tcgagcacga ggtcgatgcg gccgtcgaga tcctcgatca cgtgcgacgc
     2581 ctgcgtggcg ctcggcctgg agaagagatt ggcgctcggc gcggcgacgg gaacggcagc
     2641 ggccgcgatg agcgcgcggg cga
//
LOCUS       DBNGSH010000951         2955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3523522, whole genome shotgun sequence.
ACCESSION   DBNGSH010000951 DBNGSH010000000
VERSION     DBNGSH010000951.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3523522"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..2541
                     /locus_tag="AC16CY_21785"
     CDS             31..2541
                     /locus_tag="AC16CY_21785"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3356498.1"
                     /translation="MPPPNGPSQPPATSGSRGSGTSRLSRSSGDPSAHAPASAPAGAP
                     RRALPVDEALKIARQIADAVEYAHDRGIIHRDLKPANIKVNPDGSVKVLDFGLAKALD
                     ESGPAAHPHGTMSPTLSLTATHAGVILGTAAYMAPEQAKGRPADRRSDVWAFGVVLYE
                     MLTGQRMFGGDSVAETLASVMKEQITLADLPAETPHTVRRLVARCLDRDPRRRLQSIG
                     EARIVIEDAIEGVVVEEAAPQPVLASRPAASNWPWALAAVAGLAAVGAVAWAWSRPVA
                     TAAAVARFTVPPPNGTTITGYRSQASNLAVSPNGKYVAFVADEAGRDRTIWVRALDSL
                     IAQRLDRTEGATYPFWSPDSQHIAYFANGSLMRISVAGGAPLRICDALDGEGGTWFQE
                     EGEDGIIVFAPTQSGGLQRVLAQGGVAAPVTKLAEGELAHTFPQFLPDGRRVLYLARG
                     AKQALYVQSLGSDERTFVMNSLGRAAFSPPGFLLYLRDGTLLAHRFNLDTLGLEGEPI
                     SIAQDVRFGGGNNGRNAFGISSNGVLAYRSGGFGGNAQVTWYTRDGKADGSALESGEY
                     GPIQLSPDDKRLVVIRGSADDRDLWLKELTSGVLSRLTSAAGAEVDPVWSADSRRVAY
                     VGSSEGKRGLFVTLIGSGKHTPIPGAGEDSLEDWADKYLLTRRGGAISLLPAPEESPS
                     SVSAAIKAQEVLNEPYAIDTLQVSPDGKWVAYASLESGQPEVIVASFPAFTDRRQIST
                     GGSGAVQPRWRVDGRELFFVTRDEQLMAVDVKAGATLDTGPVRTLFQTAINTASQVHM
                     YAVTRDGKRFLIREPVGRETGAVEQLYVVTNWTSLLRR"
     gene            complement(2542..>2955)
                     /locus_tag="AC16CY_21790"
     CDS             complement(2542..>2955)
                     /locus_tag="AC16CY_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR3356499.1"
                     /translation="FQKRGGKMIVTIGTNDTLASPGAQLDYFQSVVDRMGRASVDRFA
                     RFFVMPQTGHGLTGTSYGVDGNGREIPSQPIPNRFDQLGLLFDWVENNVAPGMSVTVT
                     AGDKSLPLCSYPSYPRHQGGPSASATSYECATAQR"
BASE COUNT          474 a          996 c          993 g          492 t
ORIGIN      
        1 ctgatcgccc tcgcgcccga gcgggcagat gtgcctccgc ccaacgggcc gtcacagcct
       61 ccagcaacat cgggttctcg cgggagcggg acgagccgac tctcgcggtc gagtggcgat
      121 ccgtctgcac acgctcctgc gtcggcgccc gcgggcgccc cacgacgcgc actgcccgtg
      181 gacgaggctc tgaagatcgc cagacaaatc gccgatgccg tagagtacgc gcacgaccgc
      241 ggcatcatcc atcgcgacct caagcccgcc aacatcaaag tcaatcccga cgggtcggta
      301 aaggtcctcg acttcggcct tgcaaaggcg ctcgacgagt cgggcccggc ggctcacccc
      361 cacggcacga tgtcgccgac gctgtcgctc accgcgacgc acgcgggcgt gatcctcggc
      421 acggccgcct acatggcgcc ggagcaggcc aagggcaggc ccgccgatcg gcgctccgac
      481 gtgtgggcgt tcggcgtcgt gctctacgag atgctcacgg gccagcgcat gttcgggggc
      541 gactcggtgg cagagacgct ggcgagcgtc atgaaagagc agatcacact tgctgacctg
      601 ccggcggaga cgccgcacac cgtgcgcaga ctggtggccc gatgcctcga tcgcgatccc
      661 cgccgacgcc tgcagtcgat cggcgaagcg cgcatcgtca tcgaagacgc gatcgagggc
      721 gtggtggttg aagaggctgc gccccagccc gtgctggcgt cccgaccagc ggcgagcaac
      781 tggccctggg ccctggcggc ggtcgcgggt ctggccgccg tgggtgcagt ggcgtgggcc
      841 tggtcgcgcc ccgttgccac cgctgcggcc gtggcgcgct tcaccgtgcc cccgcccaat
      901 ggcacgacga taaccggcta tcggagtcag gcgtcgaacc tggcggtttc gccgaacggc
      961 aagtacgtgg ccttcgtcgc cgacgaggca ggtcgcgatc gcaccatctg ggtccgcgcc
     1021 ctcgactcgc tcatcgcgca gcggctggat cgtacggagg gcgcgacgta tcccttctgg
     1081 tcccccgatt cgcagcacat cgcgtatttc gccaacggca gcctgatgcg catctcggtg
     1141 gcgggcggcg ctccgctgag gatctgtgac gcccttgacg gcgaaggcgg gacgtggttc
     1201 caggaggaag gcgaggacgg gatcattgtc ttcgcgccca cccagagcgg cggactgcag
     1261 cgcgtgcttg cgcagggcgg cgtcgccgcg ccggtcacga agctcgccga gggcgagctt
     1321 gcccacacct ttccgcagtt cctgcccgac ggcaggcgcg tcctctacct ggcgcgcggg
     1381 gcgaagcagg cgctctacgt gcagtcactc ggctcggacg agcgcacgtt cgtcatgaat
     1441 tctctgggcc gggccgcgtt ctcaccaccc ggcttcctgc tgtacctccg ggatgggacg
     1501 ctcctggctc accgcttcaa cctcgacacg ctcggcctcg agggcgagcc gatctctatc
     1561 gcccaggacg tccggttcgg cggcggcaac aacggtcgaa acgcctttgg catatcgtcg
     1621 aatggagtgc tggcctatcg gagcggcggt ttcggaggca acgcacaggt cacctggtac
     1681 acccgtgacg gcaaagccga tggctccgcg ctggaatcgg gcgagtacgg gccgattcag
     1741 ctctcgcccg acgacaagcg cctcgtcgtc atacggggca gcgcggacga ccgcgacctg
     1801 tggctgaagg agttgacgag cggcgtcctt tcgcgtctca cgtcggcggc tggagccgag
     1861 gtggacccag tctggtctgc tgactcgcgt cgcgtcgcgt acgtgggcag ctcggaaggc
     1921 aagcgtggcc tgttcgtgac gctcatcgga tcgggcaagc acacgccgat cccgggcgcc
     1981 ggcgaggatt ccctggaaga ttgggctgac aagtatcttc tgactcgccg tggcggcgcc
     2041 atcagccttc tccccgctcc ggaggagagt ccttccagcg tgagcgcagc catcaaggcg
     2101 caggaggtgc tcaacgagcc ttacgcgatc gacacgctcc aggtgtctcc tgacggcaag
     2161 tgggtggcct atgcatctct cgagtcgggt caacccgagg tcatcgtcgc gtccttccct
     2221 gccttcaccg accggcgcca gatctcgacc ggtggctctg gcgccgtgca gccgagatgg
     2281 cgcgtcgacg ggagggagtt gttcttcgtc acccgcgacg agcagcttat ggccgtggac
     2341 gtcaaggcgg gcgcgacgct cgataccgga ccagttcgaa cgctctttca gacagccatc
     2401 aataccgcct cacaggtgca catgtacgcc gtgacgcgcg acggcaagcg ttttctgatc
     2461 cgcgaaccgg tgggccgtga gaccggcgcc gtcgagcagc tctacgtggt gacgaactgg
     2521 acgtcgctgc tccgacggta gttatcgctg cgcggttgcg cactcgtacg aggtcgcgga
     2581 cgccgaaggc cctccctggt gacgcggata cgacggatac gagcacagcg gcaggctctt
     2641 gtcgcccgct gtcaccgtca cggacatccc gggcgccacg ttgttttcaa cccaatcgaa
     2701 gaggaggccg agctggtcga agcgattcgg aatcggttgt gacgggattt cgcggccgtt
     2761 gccgtcgacc ccgtagctgg tgccggtgag tccatgccct gtctgcggca tcacgaagaa
     2821 gcgggcgaac cgatcgacgc ttgcgcggcc catccgatcc acgaccgact gaaagtagtc
     2881 gagctgcgcc ccgggcgacg ccagcgtgtc gttggtgccg atggtgacaa tcatcttgcc
     2941 gcctcgtttc tgaaa
//
LOCUS       DBNGSH010000952         1926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1656109, whole genome shotgun sequence.
ACCESSION   DBNGSH010000952 DBNGSH010000000
VERSION     DBNGSH010000952.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1656109"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="AC16CY_21795"
     CDS             <1..986
                     /locus_tag="AC16CY_21795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3356500.1"
                     /translation="WDYRRTLSAQADDFDLFHIVDHSYAHLVTVLPPDRSLVMCHDVD
                     AFAGVLPGTGGQSMVGRILGEQLLAGLVSARKIVCGSRATRDALVASGLVEPSRLAVV
                     PYGLHPTCRPRPDPRADAVADEQLGPIDPARPEILHVGSTVPRKRIDVLLDVFAALRD
                     RHPSVRLIRVGGAFTRSQQRHVSRLGLGGHVTVLPYLERRVLAAVYRRAAVVLQPSDR
                     EGFGLPVAEAMACGTPVVASDLPPLREVGGTMASYCAVGDVDSWVSTVGGLLDERAGD
                     AARWAARKAAAAADSRRFDALTHAQRMLDVYRELLPALPKVDLAGPIAASR"
     gene            983..>1926
                     /locus_tag="AC16CY_21800"
     CDS             983..>1926
                     /locus_tag="AC16CY_21800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3356501.1"
                     /translation="MTRPRVLHFGKYYPPHKGGIETVVETLCRGEKRWAETQALVTRR
                     ERSTVFEVIDDVPVRRVGSAATVGAVSIAPTLPMWLARADADILVLHEPNPMALLAYC
                     LARPAIPLVVWYHSEVIRPQWKYRLLYQPLLDFALRRAARVVVASPPMLDVEALAPYR
                     AKCVVVPYGLPLERYSPAPAVLARADALRVEAVRPTLLFVGRLVRYKGVEVLLRAMPG
                     LDADAVVIGDGPCRASLESVARELGLGARVRFLGEVEGDELLAWYHACDVLVLPSITR
                     QEAFGMVQLEAMLCARPVVSTEVGTGVSWVNQHERTGLV"
BASE COUNT          253 a          650 c          676 g          347 t
ORIGIN      
        1 tgtgggacta tcgacgcacg ctcagcgccc aggccgacga cttcgacctc ttccacatcg
       61 tggatcacag ctacgcgcat ctggtcaccg tgctgccgcc cgatcgatcg ctcgtgatgt
      121 gtcatgacgt cgacgccttc gccggcgtgc tgccaggcac cggggggcag tcgatggtgg
      181 ggcggatcct cggcgagcag ctgcttgcgg gactcgtctc ggcacggaag atcgtgtgcg
      241 gaagccgggc gacgcgtgac gctctggtcg cgtctggcct ggtcgagccg tcgcgtctcg
      301 ccgttgtccc gtacgggctg catcccacgt gcaggccgcg gccagacccg cgtgccgacg
      361 cggtcgctga cgagcagctc ggtccgatcg atccggcgcg tccggagatc ctgcacgtcg
      421 gaagcacggt tccgcgcaag cggatcgatg tgcttctcga cgtgttcgcc gcgttgcgcg
      481 atcgccatcc atccgtcaga ctgattcggg tcgggggggc cttcacgcga agccagcaac
      541 ggcacgtatc ccgtctgggc ctgggaggtc acgtcaccgt gctgccgtac ctcgaacggc
      601 gggtgctggc tgcggtctat cgtcgagcgg ccgtcgtcct gcagccgtcc gaccgtgagg
      661 gcttcggatt gcccgtggcc gaggccatgg cctgcggaac cccggtggtc gccagcgacc
      721 ttccgccgct gcgcgaggtc ggcgggacga tggcatcgta ctgtgccgtg ggcgacgttg
      781 attcgtgggt ctcgacggtt ggggggctgc tggacgaacg cgccggcgac gctgcccgat
      841 gggcggcgcg gaaggcagcc gctgccgccg attcacgtcg attcgacgcg ctcactcacg
      901 ctcagcggat gctcgacgtg taccgcgaac ttctgcccgc gctgccgaag gtcgacctcg
      961 ccggtcccat cgccgcctcc agatgacccg cccgcgcgtc ctccacttcg gtaagtacta
     1021 tccgccgcac aagggcggga tcgagaccgt tgtcgaaacc ctttgccgcg gggagaagcg
     1081 ctgggcggag acgcaggcgc tcgtgacccg cagggaacga tcgacggtct tcgaggtgat
     1141 cgacgacgtg ccggtccgac gcgtcgggag cgcggccacg gtcggggccg tctcgatcgc
     1201 acccacgctt ccgatgtggc tcgcgcgggc cgacgccgac atcctggtgc tccacgagcc
     1261 caacccgatg gcgctgctgg cgtactgcct ggctcgcccg gccattccgt tggtcgtctg
     1321 gtatcacagc gaggtgatcc ggccccaatg gaagtaccga ctgctctatc agccgctgct
     1381 cgacttcgcc cttcgccgcg ccgcgcgagt cgtggtggcg tcgcctccga tgctcgacgt
     1441 cgaagccctc gcgccgtatc gtgccaagtg cgtcgtcgtt ccgtacggat tgcctctcga
     1501 acggtattcg ccggcgcccg cggtgttggc gagggccgac gcgctgcgtg tcgaggccgt
     1561 caggccgacg ctgctgttcg tcggacgact ggtccgctac aagggcgtcg aggttctgtt
     1621 gagggcgatg cccggactcg acgctgacgc ggtcgtcatc ggcgacgggc cgtgccgcgc
     1681 gtcgctcgaa tctgtggcgc gcgagctggg ccttggcgcg cgtgtgcggt ttctcggcga
     1741 ggtcgaaggc gacgagttgc ttgcctggta tcacgcctgc gacgtgctgg tgctcccctc
     1801 gatcacgcgc caggaagcct tcgggatggt gcagctcgag gccatgctct gcgcgcgccc
     1861 ggtcgtcagc acggaggtcg ggacgggcgt ctcctgggtc aatcaacacg agcgcaccgg
     1921 ccttgt
//
LOCUS       DBNGSH010000953         6886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_807831, whole genome shotgun sequence.
ACCESSION   DBNGSH010000953 DBNGSH010000000
VERSION     DBNGSH010000953.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_807831"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16CY_21805"
     CDS             <1..188
                     /locus_tag="AC16CY_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356502.1"
                     /translation="RSRVSTIHRDGTLSYESAIEFDRPHALEEMVNASPADEVDRHQS
                     QPEPDEDVYILDADNDW"
     gene            178..696
                     /locus_tag="AC16CY_21810"
     CDS             178..696
                     /locus_tag="AC16CY_21810"
                     /inference="COORDINATES: protein motif:HMM:NF015908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HIR3356503.1"
                     /translation="MTGNDGDLTRAEDSGRTREVRGGEVVVSASAHDVLVSAPVGSCV
                     AVCLWDPAGVGGLLHFLWPDSKLDPARAESEPASYADTGLVLLFEQAARVGADKRRCK
                     VRLVGGAECPGRDESDRWAKRNLLAARSVLWRSGVLLDGEEVGGTKARRATLTVADGR
                     LSVTIDEPPTGQ"
     gene            719..1138
                     /locus_tag="AC16CY_21815"
     CDS             719..1138
                     /locus_tag="AC16CY_21815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3356504.1"
                     /translation="MSQNKPAAAQAKALNILIVDDSATMRALLHRVVGLADLPIGTIY
                     QAPNGQEALKILETCSVQALFTDLNMPVMNGMELLREVAKRDSWKNILRIIISTDGSK
                     LRREEARELNVDLYVEKPFRPEVVRDVLCQIAGADAR"
     gene            1101..1628
                     /locus_tag="AC16CY_21820"
     CDS             1101..1628
                     /locus_tag="AC16CY_21820"
                     /inference="COORDINATES: protein motif:HMM:NF025070.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HIR3356505.1"
                     /translation="MSSVKSPGLTPADLYAVLQTALVEVSENAFFVFVEKCDARQFAA
                     LAEQVPVRGAAGHGRSWLKSSIDFAGAFSGSVEIALPERLGFWLVASLLGMSPDETLE
                     DAQMFDGVGEFTNMVCGACLSRLGDASLFELRVPAVTRMAVEWNPLVEAPGRAELASL
                     MVSLNESPMRVRVLA"
     gene            1635..2009
                     /locus_tag="AC16CY_21825"
     CDS             1635..2009
                     /locus_tag="AC16CY_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3356506.1"
                     /translation="MSVRKKILLVDDSQTALTLERMLLAQSAYDVVLARDGREAVRQA
                     VDHTPDLIILDVVMPNMDGFEACQEIRRRESTRSIPVIMVTTRGEGHHVETGFKVGCN
                     DYVTKPINGPELLSKVRNLLGD"
     gene            2052..2696
                     /locus_tag="AC16CY_21830"
     CDS             2052..2696
                     /locus_tag="AC16CY_21830"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3356507.1"
                     /translation="MSTEHTRESFSSDADPEDGPASDYVRSLQDENQKLRALLAAQQT
                     GSEDHTARLASLYVAVNSVHSALDRRGVLSTVRDIVTTLVGSEEMALFEVDAAQGRLT
                     LLSFEGIDPSAYQDVRIGEGAIGAVALTGEALIRLEGGSADGTGDETLSACIPLKLGE
                     RVIAVLAVFRLLPQKGRFDTSDLELFDALTAHVAVALVFARLYAESQRSPGASM"
     gene            2693..3220
                     /locus_tag="AC16CY_21835"
     CDS             2693..3220
                     /locus_tag="AC16CY_21835"
                     /inference="COORDINATES: protein motif:HMM:NF015908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HIR3356508.1"
                     /translation="MSSRTSEASTAPGPDAAWAPLPRVGNLESVYLHAGHLLVSRTPC
                     RVTTVLGSCVSVGIWDPAAGIGGLNHFLLPHGAAQGARVTRYGTAAMRALIDAVLAAG
                     GRRNGLQAKLFGGACVLRSFQKSGWHLGTQNVAVARAVLLDEGIPLVAEDVEGERGRK
                     LIFQTHDGSAWVRTL"
     gene            3222..4955
                     /locus_tag="AC16CY_21840"
     CDS             3222..4955
                     /locus_tag="AC16CY_21840"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HIR3356509.1"
                     /translation="MDQMPIDHVELVQIFSAESEENLREMEVGFVQLESTPADAETLQ
                     AIFRAAHTIKGNAAGLGFSALAKFAHGVEDVLDGLRNGANVLTTPLATTLLEAVDALR
                     RLVADAVKGTDALLREHVDLLEDLRVEASLALVGDGSVLQAGSERRESDRRIGTGRRW
                     RDLHDGRSRTLRVAIEKLDRIVNLTGEIAVARERLADLLDGHDRMRRTEEILEVHRAA
                     DRLFMDLQEEVMKVRMVPLGPTFRQFVRTIRDVATAQGKQAYLELSGEDVDVDMNVIE
                     HLRDPLTHIVRNALDHGIETPEERRALGKPSSGRIFLQAWHEGGSIVIRVQDDGAGLD
                     RARVIERARAMGYGADVEKLPDGDLFRLILDAGFSTASEVTEYSGRGVGLDVVRRNVE
                     QLRGSISIESRAGAGTSFTLRLPLTLAIIRGFSVGVEDETYVMPLDAVVECIEFPQAN
                     RADQSSRGVINLRGQALPYLRLRDCFRLGGDRPERENVLVLRHHDQRVGLVVDRLFGE
                     SQTVIKPLNRVLGELPGVSGSAILGNGRVALILEVDGLMRAALRHGASESGLPASGTT
                     TQGWTGDSAVS"
     gene            4978..6621
                     /locus_tag="AC16CY_21845"
     CDS             4978..6621
                     /locus_tag="AC16CY_21845"
                     /inference="COORDINATES: protein motif:HMM:NF024140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR3356510.1"
                     /translation="MKGMFRNWTIGKKLTGAFVLMSLLVAAVGLIGSANVRQLREDAN
                     VITTSAREAGRFAVIQVDMLRQIAAEKNYILSGDQKHRQNHERLGQAVASAIDAEIRK
                     ARESGNAKRAASLEQVKAKSLGYEGTFQDVAALVSAKQVNEAVNVSLNESEIRADQMV
                     SALGDLIVENDAQLELVAQTSIMTVIVMVSVLLAVLFGLRLSRSISRPLRELARAAEG
                     VAAGDLGARVAVDNHSEVGQVADAINSMISKLLEVVSEVRAGAANLAAASAQVSATTQ
                     SLSRGTSEQAASVQETTSNIEQMNASISQNADNSRQMEQMASKGVKDGEESGQAVRET
                     VTAMKSIAEKVSIIEEIAYQTNLLALNAAIEAARAGDHGKGFAVVAAEVRKLAERSQS
                     AAREISSLASSSVRTAERSGSLLSELVPAIRKTADLVQEVAAASREQASGISQVNKAI
                     GHMDQVTQRNAAAGEQLASTAEEVASQAESLQQLISFFKVNGAHGHNGQSGHSRDAHR
                     SALASATQAAALHSLVAERAAKASAAVDPSAVDSEFQRF"
     gene            6631..>6886
                     /locus_tag="AC16CY_21850"
     CDS             6631..>6886
                     /locus_tag="AC16CY_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR3356511.1"
                     /translation="MSSDERPVGQRQYLTFFLGDEQYAVGVLGVKEIIEYGIVTKVPA
                     TPPYIRGVINLRGSVVPVVDLSVKFNLPVHPVSRRTCIVIM"
BASE COUNT         1168 a         2209 c         2472 g         1037 t
ORIGIN      
        1 tgcgatcgcg ggtgtccacc attcacaggg acggcacgtt gagctacgag tcggcgattg
       61 agttcgatcg gccgcacgcg ctcgaggaaa tggtcaacgc gtctccggca gacgaggtcg
      121 atcgtcatca gtcgcagccc gagccggacg aggacgtcta catcctcgac gccgacaatg
      181 actggtaacg acggcgacct gacgcgcgcg gaggacagtg gccgcacgcg cgaggtccgc
      241 ggcggcgaag tcgtcgtgtc ggccagcgcg cacgatgtgc tggtgagcgc gcccgttggc
      301 agctgcgtgg cggtctgcct gtgggatccg gcgggcgtgg ggggcctgct gcactttctc
      361 tggcccgact cgaagctgga cccggcgagg gccgagtccg agccggcgtc gtatgccgac
      421 accggcctcg tgctcctgtt cgagcaggcc gcgcgcgtcg gcgccgacaa gcggcgctgc
      481 aaggtgcggc tggtgggcgg ggcggagtgc ccgggccggg acgagtcgga tcggtgggcg
      541 aagcgcaacc ttcttgcggc ccgcagcgtc ctgtggcgga gcggcgttct gctcgacggc
      601 gaggaagtcg ggggcacgaa ggctcgaagg gcgacgctga cggtggcgga cgggcggctc
      661 tcggtgacga tcgacgagcc gccgaccggg cagtgacgaa ctactggcag gagtcgacat
      721 gagtcagaac aaaccagcgg cagcgcaggc gaaggcgctg aacattctca tcgtggacga
      781 ttcggcgacg atgcgcgcgc tgctgcaccg cgtcgtcggg ctggccgacc tgccgatcgg
      841 cacgatctac caggccccga acggccagga ggctctgaag atcctggaaa cctgcagcgt
      901 gcaggcgctg ttcacggatc tcaacatgcc cgtcatgaac gggatggaac tgctccggga
      961 agtcgccaag cgagacagct ggaagaacat cctccgcatc atcatctcga cggacggatc
     1021 gaagctgcgc cgcgaggaag cccgggagct caacgtggac ctgtacgtcg agaaaccttt
     1081 ccgtccggag gtggtgcgcg atgtcctctg tcaaatcgcc ggggctgacg cccgctgacc
     1141 tgtacgccgt cctgcagacg gcgctggtcg aggtgagcga gaacgcgttc ttcgtgttcg
     1201 tcgaaaagtg cgacgcccgg cagtttgccg cgctcgccga gcaggtaccg gtacggggtg
     1261 cggccggaca cggccgctcg tggctgaagt cgtccatcga tttcgccggc gcgttctcgg
     1321 gttcggtcga gatcgcgctg ccggaacgtc tgggcttctg gctggtggcg tcgctgctgg
     1381 gcatgtcgcc cgacgagacg ctcgaggacg cgcagatgtt cgacggcgtc ggcgagttca
     1441 ccaacatggt gtgcggcgcc tgcctgagca ggctcggcga cgcgtcgctc ttcgagctgc
     1501 gtgtgccggc cgtgaccagg atggcggtcg aatggaaccc gctggtcgag gctccggggc
     1561 gtgccgagct ggccagcctg atggtgtccc tgaacgaatc accgatgcgg gttcgggtgc
     1621 tcgcgtagga ggggatgagc gtgcgcaaga agatcctgct cgtggacgac tcgcaaacgg
     1681 cgctgacgtt ggagcggatg ctgttggccc agagcgcgta cgacgtcgtg ctcgcccggg
     1741 acggccgcga ggcggtgcgg caggcggtgg accacacgcc ggacctgatc atcctcgacg
     1801 tcgtcatgcc caacatggac ggcttcgagg cgtgtcagga gatacgccgg cgcgagtcca
     1861 cccgatcgat ccccgtgatc atggtcacga cgaggggaga aggccaccac gtcgagaccg
     1921 gattcaaggt cggctgcaac gactacgtca ccaagccgat caacgggccg gagctgctct
     1981 ccaaggtccg caaccttctc ggggactagg acgtagagcg ggggcttcag cccccgcgaa
     2041 cagaggcgac gatgtccact gaacacacgc gagagtcgtt ctcatccgac gcggatcccg
     2101 aggacggtcc ggcatcggac tacgtgcggt cgctccagga tgagaaccag aagctgcggg
     2161 cgctcctcgc ggcccagcag accggcagcg aagatcacac ggccaggctc gcgagcctct
     2221 acgtggccgt caacagcgtg cacagtgccc tcgaccggcg cggagtgctc tccacggttc
     2281 gcgacatcgt gaccacgctc gtcggctccg aggagatggc gctcttcgag gtggacgcgg
     2341 cgcagggccg cctcacgctc ctgtctttcg aaggcatcga tccctccgcg taccaggacg
     2401 tgcgcatcgg tgagggagcg atcggcgcgg tggcgctgac gggcgaagcg ctgatcaggc
     2461 tcgagggagg atcggccgac gggaccggcg acgagacgct gtcggcgtgc attccgctga
     2521 agctcgggga acgggtgatt gccgtgctgg cggtgttccg gttgctgccg caaaaggggc
     2581 ggttcgacac gagcgatctc gagctcttcg acgcgctcac cgcgcacgtg gctgtggcgc
     2641 tggtgtttgc ccggctctac gccgagtccc agagatctcc gggagcgtcg atgtgagttc
     2701 gcgaacgtcc gaagcgtcaa cggcgccggg cccggacgcg gcgtgggctc cgcttccccg
     2761 cgtgggaaac ctggagtccg tgtacctgca cgcgggtcac ctgctcgtgt cgcggacgcc
     2821 ctgccgggtg acgaccgtcc tcggctcctg cgtgtccgtc gggatatggg accccgccgc
     2881 cggcatcggc gggctgaacc actttctgct tcctcacggc gccgcgcagg gtgcgagggt
     2941 gacccgctac ggcacggccg cgatgagggc gctcatcgac gcggtcctcg ccgccggagg
     3001 ccgccggaac ggcctgcagg ccaagctgtt tggcggcgcc tgcgtgctgc ggtccttcca
     3061 gaaaagcggg tggcatctcg gcacgcagaa cgttgccgtg gcgcgcgccg tgctcctcga
     3121 cgagggcatc ccgttggtgg cggaggacgt cgagggggaa cgcgggcgaa agctgatctt
     3181 tcagacacac gacggctcgg cgtgggtgag gacgctctga catggatcag atgccgatcg
     3241 atcacgtcga gctcgtgcag atcttcagcg ccgagtccga ggagaacctt cgtgagatgg
     3301 aagtgggctt cgtgcagctc gaatcgacgc ccgccgacgc cgagacgctg caggccatct
     3361 tccgagcggc gcacaccatc aagggcaacg ccgccgggct cgggttctcc gcgctggcga
     3421 agttcgcgca cggcgtggag gacgtgctcg acgggctgcg aaacggcgcc aacgtcctga
     3481 ccacgccgct ggcgaccacg ctgctcgagg ccgtggatgc gctccggcgg ctcgtggccg
     3541 acgccgtcaa gggaaccgac gcgctcctgc gcgagcacgt cgatctgctc gaggacctgc
     3601 gggtcgaggc gagtctggcg ctcgtcggcg acgggtcggt cctgcaggcc ggcagcgagc
     3661 ggcgggagag cgaccgtcgc atcggcacgg gacgccgctg gcgggatctg cacgacgggc
     3721 gctcacggac gctgcgcgtc gccatcgaga agctcgatcg catcgtgaac ctgaccgggg
     3781 agatcgccgt cgcccgcgag cggctggccg acctgctgga cggacacgac cggatgcgtc
     3841 gaaccgagga gatcctcgag gtgcaccgcg ccgccgaccg gctgttcatg gaccttcagg
     3901 aagaagtcat gaaggtgcgc atggtgccgc tcgggccgac gttccggcag ttcgtgagga
     3961 cgatccgcga cgtcgcgacg gcacaaggca aacaggcata tctcgagctc tccggcgagg
     4021 acgtcgatgt cgacatgaac gtcatcgagc acctgcgcga tccgctgacc cacatcgtcc
     4081 ggaacgcgct cgatcacggc atcgagacgc ccgaggagcg ccgggcgctc ggcaagccct
     4141 cgagcggccg catcttcctg caggcctggc acgaaggcgg gtccatcgtc atccgcgtcc
     4201 aggacgacgg tgcggggctc gatcgcgcca gggtcatcga gcgcgcgcgc gccatgggct
     4261 acggcgccga cgtcgagaaa ctgccggacg gcgatctgtt ccgcctgatt ctggacgccg
     4321 gcttctcgac ggcgtcggag gtgaccgaat actccggccg cggcgtcggc ctggacgtgg
     4381 tccgccgcaa cgtggaacag cttcgcggct cgatctcgat cgagagccgc gccggggcgg
     4441 gcacgtcctt cacgctgcgc cttccgctca cgctcgctat catccggggc ttctcggtcg
     4501 gcgtcgagga cgaaacctac gtgatgccgc tcgacgcggt ggtcgagtgc atcgagtttc
     4561 cgcaagccaa ccgggccgac cagtccagcc gcggcgtgat caacctgcgg ggccaggcgc
     4621 tgccgtacct gcggctgcgc gactgcttca ggctcggcgg cgatcggccg gagcgggaga
     4681 acgtactggt cctccgccac cacgatcagc gcgtcggcct cgtggtggat cgcttgttcg
     4741 gtgaaagcca gaccgtcatc aagccgctca accgggttct cggcgagctg cccggcgtgt
     4801 ccggctccgc cattctcggc aacggccggg tggcgctgat tctcgaggtg gacgggttga
     4861 tgcgtgcggc gttgagacac ggggcgtccg agagcggtct tccggcgtcc ggcacgacga
     4921 cgcagggctg gaccggcgac agcgccgtgt cctgaggtgt gaaggaggca aggggcgatg
     4981 aaaggcatgt ttcggaactg gacgatcggc aagaagctca ccggagcgtt cgtgctgatg
     5041 tccctgctcg tggcggccgt gggcctcatc gggagcgcga acgtgcgcca gctccgcgaa
     5101 gacgccaacg tgatcacgac gtcggcgcgc gaggcgggcc ggttcgccgt gatccaggtg
     5161 gacatgctca ggcagatcgc ggcggagaag aactacatcc tctccggcga ccagaagcat
     5221 cgtcagaacc acgaacggct cggacaggcc gtcgcctccg cgatcgacgc cgagatcagg
     5281 aaggccaggg agagcggcaa cgccaagcga gccgcgagcc tggagcaggt gaaggccaag
     5341 agcctcggct acgagggcac gttccaggac gtggccgcgc tcgtctcggc aaagcaggtc
     5401 aacgaagccg tcaacgtctc gctgaacgaa tccgagatca gggcggacca gatggtgagc
     5461 gcgctcggcg acctgatcgt cgaaaacgac gcccagctgg agctggtcgc tcagacgtcg
     5521 atcatgacgg tgatcgtgat ggtatcggtg ctgctggccg tgctgttcgg cctgcgcctg
     5581 agccggagca tcagcaggcc gctgcgggag ctggcccgcg ccgcggaggg cgtcgcggcg
     5641 ggcgacctcg gcgccagggt cgcggtggac aaccacagcg aggtcgggca ggtggccgat
     5701 gcgatcaact ccatgatctc gaagctgctc gaagtggtgt ccgaggtgcg cgcgggcgcc
     5761 gcgaatctcg ccgcggcctc cgcgcaggtc tcggccacga cgcaaagcct gtcccgtggc
     5821 acgagcgagc aggcggcgag cgtccaggag acgacctcga acatcgagca gatgaacgcg
     5881 tcgatctcgc agaacgccga caacagccgc cagatggaac agatggcgtc caagggcgtc
     5941 aaggacggcg aggagagcgg acaggccgtt cgggagacgg tgaccgcgat gaagtcgatc
     6001 gcggagaaag tgtccatcat cgaggagatc gcctaccaga cgaacctgct cgcgctgaac
     6061 gcggccatcg aggccgcacg cgcgggcgat cacggcaagg gcttcgccgt cgtcgccgcc
     6121 gaggtgcgca agcttgccga gcgcagccag tcggcggcgc gcgagatcag ctcgctggcg
     6181 tcttccagcg tgagaacggc ggagcgatcg ggaagcctgc tgtccgagct cgttcccgcc
     6241 atccggaaaa ccgccgacct cgtgcaggaa gtcgcggcgg cctcgcgcga gcaggcctcc
     6301 ggcatcagcc aggtcaacaa ggccatcgga cacatggatc aggtcacgca gcggaatgcg
     6361 gcggccggcg aacaactggc gtcaacggcc gaggaagtgg cgtcccaggc cgagagcctg
     6421 cagcagctca tctccttctt caaggtcaac ggggcccacg ggcacaacgg ccagtccggt
     6481 cactcgcgcg acgcgcatcg atcggcgctc gcgtcggcga cgcaggccgc ggcgctgcac
     6541 agcctggtcg cggaacgcgc cgcgaaggcc agcgcggcgg tcgatccgtc tgccgtggac
     6601 tccgagttcc agaggttcta gaggttccag atgtcaagcg acgaacgacc ggtcggccag
     6661 cgccagtacc tgacgttctt cctgggcgac gagcagtacg ccgtgggcgt tctgggcgtc
     6721 aaggagatca tcgagtacgg catcgtcacc aaggtcccgg cgacacctcc ctacatccgc
     6781 ggcgtcatca acctgcgcgg gagcgtcgtg cccgtcgtcg atctgtccgt caagttcaac
     6841 ctgccggtgc acccggtcag ccgccgcacc tgcatcgtca tcatgg
//
LOCUS       DBNGSH010000954         3060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3694140, whole genome shotgun sequence.
ACCESSION   DBNGSH010000954 DBNGSH010000000
VERSION     DBNGSH010000954.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3694140"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="AC16CY_21855"
     CDS             <1..1973
                     /locus_tag="AC16CY_21855"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3356512.1"
                     /translation="RTTRDLFGLVRTTMPSDNAGGLPEGTYVNIIAYILQRNGVAPGA
                     TALTAATDVRIGAAAPPAPAAAPAAAAAPGRGAAPPAAAAQAPAGRGAGAQGGGAGPG
                     RGGRGQEPPPTGLTVRGEVRNLGRVTDEMLRNPPPADWPMLRRDQFASSYSPLTQISR
                     DNVQDLQLQWVWPMNEGGTNQPSPLAKDGTIFLNNTGGIVQALDGRTGDLIWEQRLGV
                     NIAMRGMSLYEDKLYLALSNAHLVALNARTGEVVWDVQMPDGRGSSSGPLIARGKVIQ
                     GMGGCSQYVEQKCFISAYDAATGKQLWRFHTIAREGEPGGNTWGSLTNLFRAGGETWI
                     TGSYDPDLNMTYWGTAQAKPWMPVSRGMYTLDKALYTSSTVALDADTGKLAWYFSHAP
                     GEALDLDIVFERVLVDSGGQNFVFTVGKDSVIWKLDRKTGRYLGHKEGTFQNVWESFD
                     PKTGEPRYRADIIEHEIGQWIDGCPSTEGGHNWQAMSHHKQTNQLVIPLSQSCIAIRA
                     QRIEQVAGGGSGGGADRRFYEMPGTDGNIGKLAAFDVNSLKETWALQQRAPFLTSVLS
                     TGGDVVFAGDLAREFKAVDARTGRVLWRTRLGTSVQGFPISFAVDGRQYIAVTTGLGG
                     GSPRLVPNTIAPEIRVPTTGQALYVFALPERK"
     gene            2057..>3060
                     /locus_tag="AC16CY_21860"
     CDS             2057..>3060
                     /locus_tag="AC16CY_21860"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3356513.1"
                     /translation="MASMFEQWPWWVFSLLWAALTIGGAYLLGRVVNAVIARRLTGLA
                     KRTRGQWDDVVIRELTSRIPLWITLVGCWVALGYWPMGDRWLTLASNAILIVGVGSIT
                     FALSTIATTLVAAYGLQAVPGAPASHLTQNVVRLVIMSVGLLVLLNQLGIEIRPMLAA
                     LGVGGLAVALALQEPLSNLFAGLFVSMAGQVRIGDQVRLDSGLEGRVVDFNWRATWIE
                     VPSGNIAVVPNAKLSQAVVTNYSLPTTEVAVLVDIIVDHRNDAAQVERLALEVARGVL
                     SEIDGAFPGFEPVVRFHTFADTGLRATVSMRATSLAGLSALRHAFIMRLHKRFQQAGV
                     A"
BASE COUNT          517 a         1034 c         1044 g          465 t
ORIGIN      
        1 cgcgaaccac tcgcgacctg ttcggtctgg tgcgcacgac catgccctcg gacaacgccg
       61 gaggcctgcc ggaaggcacc tacgtcaaca tcatcgccta catcctgcag cgcaacggcg
      121 tcgcgccagg cgccacggcg ctgacagccg ccacggacgt gaggataggc gccgctgccc
      181 cgccggcgcc tgcagccgca cctgcagccg cggccgcacc cgggcgagga gccgccccgc
      241 ctgccgccgc ggcccaggcc ccggccgggc gaggcgcggg cgcgcaaggc ggcggcgcag
      301 gaccagggag aggaggccgt ggacaggagc cgcctccaac gggtctgacc gtccgtggag
      361 aagtgaggaa cctcgggcgc gtgacggacg agatgctccg caatcctccg ccagccgact
      421 ggccgatgct gcggcgggac cagtttgcat cgagctacag cccgctcacg cagatctcgc
      481 gcgacaacgt gcaggacctt cagctgcagt gggtgtggcc catgaacgaa gggggcacga
      541 atcagccgtc gccgctcgcc aaggacggga cgatcttcct gaacaacacg ggcggcatcg
      601 tgcaggcgct cgacggccgc acgggcgatc tgatctggga gcagcggctt ggcgtgaaca
      661 tcgctatgcg cggcatgtcg ctctacgaag acaagctgta cctcgcgctg agcaacgcgc
      721 acctggtcgc gctcaacgcg cgcaccggcg aggtcgtgtg ggacgtgcaa atgccggacg
      781 gccgcggcag ctcgagcggt ccgctcatcg cacgcggcaa ggtgatccag gggatgggcg
      841 gatgttcgca gtacgtcgaa cagaaatgct tcatcagcgc ctacgatgcg gccaccggca
      901 agcagttgtg gaggttccac accatcgcgc gcgaagggga gccgggcggc aacacctggg
      961 gcagcctcac caatctgttc cgcgcgggcg gcgaaacgtg gatcaccggc agctacgatc
     1021 ccgatctgaa catgacctac tgggggaccg cgcaggccaa gccctggatg ccggtcagcc
     1081 ggggcatgta cacgctcgac aaggcgctct acacgagctc gacggtcgcg ctcgacgccg
     1141 acaccggcaa gctggcctgg tacttctcgc atgcgcccgg tgaggcgctc gacctcgaca
     1201 tcgtgttcga gcgcgtgctg gtggacagcg gcgggcagaa cttcgtgttc acggtcggca
     1261 aggactccgt catctggaag ctcgatcgca agaccggccg gtacctcgga cacaaggaag
     1321 ggacgttcca gaacgtctgg gaaagcttcg acccgaagac gggcgagccg cggtaccggg
     1381 cggacatcat cgagcacgag atcggccagt ggatcgacgg gtgccccagt accgaaggcg
     1441 ggcacaactg gcaggcgatg agccaccaca agcagaccaa tcagctcgtc atccccctga
     1501 gccagagctg catcgccatt cgcgcgcaga ggatcgagca ggtcgccggc ggcggcagcg
     1561 ggggcggcgc cgatcggcgc ttctacgaga tgcccggtac cgacggcaac atcggcaagc
     1621 tcgccgcctt cgacgtcaac agcctgaagg aaacgtgggc gctgcagcag cgcgcgccgt
     1681 tcctgacctc ggtgctctcg acgggcggcg acgtggtctt tgccggcgac ctcgcgcggg
     1741 aattcaaggc cgtggacgcg aggacgggac gagtgctgtg gcgcacgcgc ctgggcacgt
     1801 cagttcaagg gttcccgatc tccttcgccg ttgacggccg gcagtacatc gccgtcacga
     1861 cggggctcgg cggcggcagc ccgcgcctcg tgccgaacac gatcgcgcca gagatccgcg
     1921 tgccgacgac gggacaggcg ctgtacgtct tcgcactgcc ggagcgcaaa tagccgcctc
     1981 actgcgctga cgtaccgcgg tccgggtgct acggccgtgc tcgatccacg ccggtcgagc
     2041 gcgtacactc acccccatgg cttccatgtt cgagcaatgg ccgtggtggg tgttctcgct
     2101 gctgtgggca gcgctcacca tcgggggcgc atacctcctc gggcgcgtgg tgaacgccgt
     2161 catcgcgcga cgcctgacgg gcctggccaa gcgcacgcgg ggccagtggg acgacgtcgt
     2221 gatccgcgag ctcacgtcgc ggattccgct ctggatcaca ctcgtcggct gctgggtcgc
     2281 gctcggctac tggccgatgg gcgatcgctg gctcactctt gcatcgaacg cgattctgat
     2341 tgtcggagtc gggtcgatca ccttcgcgtt gtccacgatc gcgacgacac ttgtcgccgc
     2401 ctacggactg caggctgtcc cgggtgcgcc ggcctcgcac ctcacgcaga acgttgtgcg
     2461 cctggtgatc atgtccgtcg ggctgctcgt gctgctgaac caactgggca ttgaaatccg
     2521 gccgatgctc gcggcgctgg gagtcggggg cctggcggtc gccctggcac ttcaggagcc
     2581 gctgtccaat ctgttcgccg gcctcttcgt ctcgatggca gggcaggtcc ggatcggcga
     2641 tcaggtccgg ctcgactcag gactcgaggg ccgcgtcgtc gacttcaact ggcgcgcaac
     2701 ctggatcgaa gtcccgagcg gcaatatcgc cgtcgtcccc aacgccaagc tctcgcaggc
     2761 ggtcgtgacc aactacagcc tgccgaccac ggaggtcgcc gtgctcgtcg acattatcgt
     2821 ggaccacagg aacgacgcgg cgcaggtcga acgcctggcg ctcgaagtgg cgcgaggcgt
     2881 tctgagtgag atagacggcg cgttcccggg cttcgagccg gtcgtgcggt ttcacacgtt
     2941 cgccgacaca ggacttcgcg ccaccgtgtc gatgcgcgcg acgtcgctgg ccggcctctc
     3001 tgcgctgcgc cacgccttca tcatgcggct ccacaaacgc ttccagcagg cgggcgtcgc
//
LOCUS       DBNGSH010000955         1632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1316624, whole genome shotgun sequence.
ACCESSION   DBNGSH010000955 DBNGSH010000000
VERSION     DBNGSH010000955.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1316624"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1632)
                     /locus_tag="AC16CY_21865"
     CDS             complement(<1..>1632)
                     /locus_tag="AC16CY_21865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3356514.1"
                     /translation="DAKWPLKVTSNEPDTDGWSGIRGYNYQTSPNEKRDVGVTTRRAG
                     DTWTVSIYDVEQAIGEKRGAQIALIFGRLLPKGYTRETFAGKKAHTLDAARIAELSKF
                     VESGREKLRVPGIAIGLVQDGKVVFAGGFGAKEIGKPAKPDADTLFMVASNTKAMTTL
                     MLAKLLDEGKLTWETPVTKLLPSFKLGDADTTSRVLVKHLICACTGLPRQDYEWLLQY
                     EGVTAEGALKTLATIQPTTKFGELFQYSNLLAAAAGYVGGHVAFPSLELGAAYDRAMQ
                     TRVFGPLGMASTTFDFRRALAANHAAAHAPDVDGTPARAVMEVNYSVIPVRPAGGAWS
                     SVRDMLKYVQMELDEGALPGGRRYVSKDALLARRAPQVSVGRDITYGMGLVVETTYGT
                     PVVQHGGALIGYHSDMMWLPEHNVGAVILTNADPGQRLLGPFQRKLLEVLFDGRPEAD
                     AQAAAQATTLYEQLAAERKLLTVPAAPDDAAKLAPKYTNAALGTITVRRDGGAVVFDL
                     GEWKSEMASRHNPDGTVSFLTIVPGFTGFEFVVGGA"
BASE COUNT          240 a          554 c          551 g          287 t
ORIGIN      
        1 cgcgcccccg acgacgaact cgaagccggt gaagccgggc acgatggtga gaaatgacac
       61 ggtgccgtcc gggttgtgcc gggaggccat ctcgctcttc cactcgccca ggtcgaagac
      121 gacagcgccg ccatcgcgcc gaacggtgat ggtgccgagc gcggcgttcg tgtacttcgg
      181 cgcgagcttc gccgcatcgt cgggagcggc cggtaccgtc aggagcttcc gttcggcggc
      241 gagctgctcg tagagcgtgg tggcttgagc cgcggcctgt gcatcggcct ctggacgtcc
      301 gtcgaagagc acctcgagca gcttccgctg gaacggcccc agaagccgct gtcccggatc
      361 ggcgttggtc aggatcacgg caccgacgtt gtgctcgggc agccacatca tgtcgctgtg
      421 gtagccgatg agcgcccctc cgtgctgtac gacaggagtt ccgtacgtcg tttcgaccac
      481 gagccccatg ccgtaggtga tgtcccgccc aaccgacacc tgcggcgcgc gccgcgcaag
      541 gagcgcgtcc ttcgagacgt atcgcctgcc gcccggcagc gcgccttcgt cgagctccat
      601 ctggacgtac ttcagcatgt cgcgaacgct gctccaggcg ccgcccgcgg gacgaacggg
      661 aatgaccgag tagttgacct ccatcacggc tcgcgcgggc gtgccgtcga catcgggcgc
      721 gtgcgcggcc gcgtgattgg ctgccagcgc gcggcggaaa tcgaacgtcg tcgacgccat
      781 gccgagcgga ccgaagacgc gcgtctgcat cgcccggtcg taggcggcgc cgagctcgag
      841 cgagggaaac gccacgtggc cgccgacgta gccggcggct gccgcgagca ggttcgaata
      901 ctggaacagc tcgccgaact tcgtcgtcgg ctggatggtg gcgagcgtct tcagcgcccc
      961 ctccgccgtg acgccctcgt actgcaacag ccactcgtag tcctgccgcg gcaggcccgt
     1021 gcaggcgcag atcaggtgct tcacgaggac gcggctcgtg gtatcggcgt cgccgagctt
     1081 gaacgacggc aggagtttcg tgacgggcgt ctcccacgtc agcttgcctt cgtccagcag
     1141 cttggcgagc atcagcgtcg tcatcgcctt cgtattcgac gcgaccatga agagcgtgtc
     1201 ggcatccggc ttggcgggct tgccaatctc cttcgcgccg aagccgccgg cgaagacgac
     1261 cttgccgtcc tgcaccaggc caatcgcgat gccagggacg cggagctttt cgcggccgct
     1321 ttcgacgaac ttcgagagct ccgcgatcct cgcggcgtcg agcgtgtgcg ccttcttgcc
     1381 ggcgaaggtt tcgcgcgtgt agcccttcgg caggaggcgg ccgaagatca gcgcgatctg
     1441 cgctccccgc ttctctccga tggcctgttc cacgtcgtag atggagaccg tccacgtgtc
     1501 gccggcgcgg cgcgtggtga cgcccacgtc gcgcttctcg ttcggcgacg tctggtagtt
     1561 gtatccacgg atgccggacc agccgtcggt gtcgggctcg ttgctggtca ccttgagcgg
     1621 ccacttcgcg tc
//
LOCUS       DBNGSH010000956         5517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3354720, whole genome shotgun sequence.
ACCESSION   DBNGSH010000956 DBNGSH010000000
VERSION     DBNGSH010000956.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3354720"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2222
                     /locus_tag="AC16CY_21870"
     CDS             <1..2222
                     /locus_tag="AC16CY_21870"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3356515.1"
                     /translation="TVAVVVDGVLQTTGLGFSEELYDIEQVEVLKGPQGALYGRNSSG
                     GAINITTRPPTNALEGFARIGYGNGENVVFNGAVSGALVPDTLMGRAAVSIKDADGWR
                     QNITLQKKADTYADRSVRGRLLWKPAPAFSGDLRFAYSNAEAGASQFVSNAPNFVEPP
                     PVGGLPGLAANADGRDNGSATPVPGLPASITPLVGDPNNTSVGIQGDIAGIDDREVTS
                     ISGKFDWQTEAGTLTSVTAWDKLTLLGTLETFPYFPFLQSTADPTAGTRTDAIVLPPA
                     VYGPLAAVNASTGQNRFHNAFSQEVRLTSPANQRARWILGGYFVANDLDVMISVNRDL
                     GQDAVLQDKDPNIGGVNPTATWNERFVAAVAPVYAANPGLIPPQCIGGAVPPTVCAAN
                     LANPNLNSAALSYNLDHNDNTAFAGFGQVNFDVSTQVELSFALRYDRDNRQLTLETPQ
                     TYLPVFAFPSGQEGDVREETFDAWQPKATIRWKPTPGVSVYGVYAQGFRSGGFNLSGV
                     AAGVNALRNAGVPGMPQGVTDSWDQEDTRGVEFGFKSSLLGGAASFNASGFYTNIDNA
                     FTFFFVAPYNAQTIRNIDGARASGFEADFSWLPFNGLQFDVAVGVLDTEITSSTWIGT
                     GGIDIVGKKLPFNPDSTVNVGVSYSRPIGQGWRGFTRLDWELLGETAFDPENFATRDA
                     INLLNLRGGVTAPGEWEIAAWARNLTNKDYLSENINPNGISWLGRPRQFGIELMKRF"
     gene            2456..2752
                     /gene="gatC"
                     /locus_tag="AC16CY_21875"
     CDS             2456..2752
                     /gene="gatC"
                     /locus_tag="AC16CY_21875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR3356516.1"
                     /translation="MATQLTVADVERIAALAHLELTDAEKQVFTRQLADILAYAEQLQ
                     SIDTTGVEATAHVNAHEAERDDTPRESLPVAEALANAPDPAPDGGLFRVPRVIG"
     gene            2749..4239
                     /gene="gatA"
                     /locus_tag="AC16CY_21880"
     CDS             2749..4239
                     /gene="gatA"
                     /locus_tag="AC16CY_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR3356517.1"
                     /translation="MSGLPAGAREIRDEIAAGRVSAVEVCQRALDRIAAVNPSLNAFN
                     LVDADRALARAAHIDKQRAAGAALGPLAGVPVAVKDNLCVRGMRTTASSRILDSFVPP
                     YDATVVSRLERAGAVIVGKTNCDEFAMGSSTENSAYGPAKNPWALDRAPGGSSGGSAA
                     AVAASCVPLALGSDTGGSIRQPAAFCGVTGLKPTYGRVSRYGLLAFASSLDQIGPMAR
                     SVGDAALTLSVIAGPDPMDSTSSRDAVPDFAAMLNGHAKGVRVGVPRAFVSEGVNDSV
                     RAAFDAALDQLRGVGATIVDIELPHAKYAIPVYYLICTAEASSNLARYDGVKYGHRST
                     AASQGLKAMYNRTRDEGFGPEVKRRIMLGTYVLSAGYYDAFYLKAQQVRTLLRQDYAH
                     ALESVHVVAMPTTPTPPFKLGEKTDDPLQMYLEDIFTVSVNLAGLPGISVPCGFVHRE
                     QAGAGGSQQLPLGFQLIGRMFDEATLLRVADAYQRATNWSQQTPRL"
     gene            4261..5205
                     /locus_tag="AC16CY_21885"
     CDS             4261..5205
                     /locus_tag="AC16CY_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3356518.1"
                     /translation="MTNRRDWLLQMAALGVAAGAARTVVEAQTVTDTTRFFPGFKNFR
                     VKTSGAEINGVIGGQGPPILLLHGAPQSHVSWRLVAPKLAATRTVVVPDLRGYGDSSK
                     PPDGENHSNYSKRAMALDQVEVMRSFGFEKFPVIGHDRGGRVTHRMALDHAGSVTHAA
                     VLDIVPTHYLYTHFRIEFVQRYFHWFNYLRPAPGPENDLRAQNEAARARATSEVQIEY
                     LRTAGDPANIHAMCDDYRAGASIDLAHDEADMKKKIACPLLVLWGQKGAMDGLYDVLG
                     IWRERGRKAFGKSLPAGHNLQEDVPDLVFNEIQQLLKM"
     gene            5221..>5517
                     /locus_tag="AC16CY_21890"
     CDS             5221..>5517
                     /locus_tag="AC16CY_21890"
                     /inference="COORDINATES: protein motif:HMM:NF039593.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIR3356519.1"
                     /translation="MRALVTSVCVLILLGASGPAGQRNAPAPNPPNLPPISMTCAHHP
                     DVLELRPGTCPLCKMALVPVRLDSSWMCPVHTTIIETERGSCRLCRRQLVPVTVA"
BASE COUNT          923 a         1911 c         1832 g          851 t
ORIGIN      
        1 ccaccgtggc tgtcgtcgtg gacggcgtgc tccagaccac ggggctcggc ttctccgagg
       61 agctgtacga catcgagcag gtggaggtgc tcaagggccc gcagggcgcg ctgtacggcc
      121 gcaactccag cggcggcgcg atcaacatca ccacgcggcc gccaaccaac gcgctcgaag
      181 ggttcgcgcg aatcggctac ggcaacggcg agaacgtcgt cttcaacggg gccgtcagcg
      241 gcgccctggt gcccgacacg ctgatggggc gcgcggccgt gtcgatcaag gacgccgacg
      301 gatggcggca gaacatcacg ctccagaaga aggccgacac ctacgcggat cggtcggtcc
      361 gcggccggct gctgtggaag cccgctccgg ccttcagcgg cgatctgcgg ttcgcctact
      421 cgaacgcgga agccggtgcg tcgcagttcg tgtcgaacgc gcccaacttc gtggagcccc
      481 cgccggtggg cgggctgccg ggcctggccg ccaacgcgga cgggagggac aacggctcgg
      541 cgacgcccgt tccgggcctg cccgcgtcca tcaccccgct cgtcggcgat ccgaacaaca
      601 cgagcgtcgg catccagggc gacatcgccg gcatcgacga ccgcgaggtc acctcgatct
      661 cgggcaagtt cgactggcag accgaggccg gcacgctgac ctcggtgacc gcgtgggaca
      721 agctcacgct gctcggcacg ctcgaaacct tcccgtactt cccgttcctg cagtcgacgg
      781 cggatccgac ggccggcacg cggaccgacg cgatcgtgct gccaccggcc gtctacggcc
      841 cgctcgcagc ggtgaacgcc agcacgggac agaaccggtt ccacaacgcg ttcagccagg
      901 aggtgcgcct cacgtctccg gccaaccagc gggcgcgctg gattctcggc ggctacttcg
      961 tcgccaacga tctcgacgtg atgatctcgg tcaaccgcga tctcgggcag gacgcggtgc
     1021 tgcaggacaa ggacccgaac atcggcggcg tgaacccgac ggccacgtgg aacgagcgct
     1081 tcgtcgcggc ggtcgctccg gtgtacgccg ccaacccggg cctcatcccg ccacagtgca
     1141 tcggcggggc ggtgccgccc acggtctgcg cggcgaacct cgccaacccg aacctgaact
     1201 ccgcggcgct ctcctacaat ctcgaccaca acgacaacac ggcgtttgcc gggttcggac
     1261 aggtgaactt cgatgtgagc acacaggtgg agctgtcgtt cgcacttcgc tacgaccgtg
     1321 acaaccggca gctcacgctc gagacgccgc agacctactt gccggtattc gccttcccga
     1381 gcggccagga gggcgacgtc cgcgaggaga ccttcgacgc gtggcagccg aaagccacga
     1441 ttcggtggaa gccgacgccc ggggtctccg tgtacggcgt ctacgcgcag gggttccgga
     1501 gcggcgggtt caacctgagc ggcgtggccg cgggcgtcaa cgcacttcgc aacgccggcg
     1561 tgcccggcat gccccagggc gtcaccgatt cgtgggacca ggaagacacg cgcggcgtgg
     1621 agttcggctt caagtcgagc ctgctggggg gcgcggcgtc gttcaacgcg tcgggcttct
     1681 acacgaacat cgacaacgcc tttaccttct tcttcgtcgc accgtacaac gcgcagacca
     1741 tcaggaacat cgacggcgcc agggccagcg gcttcgaggc cgacttctcg tggctgccgt
     1801 tcaacgggct gcagttcgat gttgcggtgg gggtgctcga caccgagatc acgagcagca
     1861 cctggatcgg cacgggcggc atcgacattg tcggcaagaa gctgcccttc aaccccgact
     1921 cgaccgtcaa cgtcggcgtc agctattccc ggccgatcgg ccagggatgg cgcggcttca
     1981 cgcgactgga ctgggagctg ctcggggaaa ccgcgttcga tcccgagaac ttcgcgacac
     2041 gcgatgcgat caacctgctg aacctgaggg gcggggtgac cgctcccggc gagtgggaga
     2101 tcgcggcctg ggcgaggaac ctgacgaaca aggactacct gtcggagaac atcaacccga
     2161 acggcatctc gtggctcggc aggccgcgcc agttcggcat cgagctgatg aagcgcttct
     2221 agggctggag agaagactgg cgggcttttc cggtccgtcc ctggtccccg gtcctgaacg
     2281 ggaccaggga ccaagggacc aaggcttcag ttgggcctga acagatctct ccgcccatga
     2341 tcatggtcaa gagctggtcc gcctcccttt tcagtgtgcg gtcgaattgt ccatcggttc
     2401 gcgaatgcga aggccccacg gggcccggga gcctcccgag tggtaccatc gagccgtggc
     2461 cactcagctg accgtcgcag acgtcgagcg catcgccgcg ctcgcgcacc tcgagctcac
     2521 tgacgccgag aagcaggtct tcaccaggca gctcgccgac atcctcgcct acgccgaaca
     2581 actgcagtcg atcgacacga ctggcgtcga ggcaaccgcg cacgtcaacg cgcacgaggc
     2641 agaacgagac gacacaccgc gggaatcgct gccggtcgcc gaggcgctcg ccaacgcgcc
     2701 cgaccccgcg ccggacggcg gcttgttccg cgttccccga gtcatcggat gagcggcctc
     2761 cccgccgggg cccgcgaaat tcgcgacgag atcgccgccg ggcgtgtctc cgcggtggag
     2821 gtctgccagc gcgcgctcga tcggatcgcg gccgtgaacc cctcgctcaa cgctttcaac
     2881 ctggtggacg ccgatcgcgc tctcgcacgg gcggcccaca tcgacaagca gcgtgcggcc
     2941 ggcgccgccc tgggcccgct ggccggcgtg ccggtcgccg tcaaggacaa cctctgcgtg
     3001 cgcgggatgc gcacgaccgc ctcgtctcgc attctcgact cgttcgttcc gccgtacgac
     3061 gcaacggtcg tgtccaggct cgagcgggcc ggagcggtca tcgtcggcaa gaccaactgc
     3121 gacgagttcg ccatgggctc ttcgaccgag aactcggcgt atgggcccgc gaagaacccc
     3181 tgggctctcg accgcgcgcc cggggggtcg agcggcggat cggcggcggc cgttgccgcc
     3241 tcgtgcgtgc cgcttgcgct cggctccgat acgggcggct cgatcaggca gccggccgcc
     3301 ttctgcggcg tcaccggcct caagccgacc tacggtcgcg tctcgcgcta cggcctgctg
     3361 gcctttgcgt cgtcgctcga tcagatcggc ccgatggcgc gcagcgtagg cgatgccgct
     3421 ttgacgctct cggtcatcgc ggggcccgac ccgatggact cgacctcgtc ccgggacgcc
     3481 gtgcccgatt tcgccgccat gttgaacgga catgccaagg gagtgcgggt cggcgtgccg
     3541 cgggcattcg tgagcgaggg agtgaacgac agcgtccgcg cggccttcga cgccgctctc
     3601 gatcagctgc gcggcgtggg cgccacgatc gtggacatcg agctcccgca cgcgaagtac
     3661 gccatcccgg tctactacct gatctgcacg gcggaggcga gctccaatct cgcgcgctac
     3721 gacggagtga agtacggcca ccgttcgacg gccgcgagcc agggcctcaa ggcgatgtac
     3781 aaccggacca gggacgaggg cttcgggccg gaggtcaagc ggcggatcat gctcggcacc
     3841 tacgtcctga gcgccggcta ctacgacgcg ttctacctga aggcccagca ggtccggacg
     3901 ctgctccgac aggactacgc ccacgcgctc gagtcggtcc acgtcgtcgc gatgccgacg
     3961 acgccgacgc cgccgttcaa gcttggagag aagacggacg acccactcca gatgtacctg
     4021 gaggacatct tcaccgtcag cgtgaacctg gccgggctgc ccgggatcag cgtcccgtgc
     4081 ggcttcgtcc atcgggaaca ggcgggcgca ggcggctctc agcagttgcc gctcggcttt
     4141 cagttgatcg gccggatgtt cgacgaggcg acgctgctgc gcgtggccga cgcctatcag
     4201 cgcgccacca actggagtca gcagacacct cgcctttgac ctttgcccta caatgactcc
     4261 atgaccaacc gccgcgactg gctcctgcaa atggccgcgc tcggcgtggc cgccggggcc
     4321 gcccggacgg tcgtcgaggc gcagaccgtg accgatacga cgcgcttttt tccaggcttc
     4381 aagaacttcc gggtgaagac gagcggcgcc gagatcaacg gcgtgatcgg cggccagggc
     4441 cccccgatcc tccttctgca cggcgcgccg cagtcgcacg tctcgtggcg cctggtggcg
     4501 ccgaagctgg cagcgacgcg cacggtcgtc gtgcccgatc tgcgcggata cggcgacagc
     4561 agcaagcccc ccgacggcga gaatcactcg aactactcga agcgcgcgat ggcgctggat
     4621 caggtcgaag tgatgcgctc cttcggcttc gagaagtttc ctgtgatcgg ccacgaccgc
     4681 ggtggacgcg tgacgcacag gatggcgctg gatcatgcgg ggtcagtcac gcacgcggcc
     4741 gtcctggaca tcgtaccgac gcactacctg tatacgcatt tcaggatcga gttcgtccag
     4801 cgctactttc actggttcaa ctacctgcgt ccggcacccg gcccggagaa cgacctgcga
     4861 gcgcagaacg aggcggcccg cgcgcgggcg acctccgagg ttcagatcga gtacctgcga
     4921 acggccggcg acccggcgaa catccacgcg atgtgcgatg actaccgcgc cggggcctcc
     4981 atcgatctgg cgcacgacga agccgacatg aagaagaaga tcgcgtgccc gctgctcgtg
     5041 ctctggggac agaagggcgc gatggacggg ctctacgacg tgctcgggat ctggcgtgag
     5101 cgcggcagga aagcgttcgg caagtcgctg ccggccggac acaatctgca ggaggatgtg
     5161 ccggatctgg tcttcaacga gatccagcaa ctcttgaaga tgtgacctga ggcgtctgcc
     5221 atgcgcgcac tggtgacctc cgtctgcgtg ctgatcctgc tcggggcatc cggcccggcc
     5281 gggcagcgga atgcgcctgc gccgaatccc ccgaacctcc cgcccatctc gatgacgtgc
     5341 gcgcatcacc cggacgtcct cgagctccgc ccggggacat gtccgctctg caagatggcg
     5401 ctcgtccccg tacggctcga ttcgtcctgg atgtgccccg ttcacacgac catcatcgag
     5461 accgagcgcg gttcgtgccg gctgtgccgc aggcagctcg tcccggtcac cgtggcg
//
LOCUS       DBNGSH010000957         6380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3863539, whole genome shotgun sequence.
ACCESSION   DBNGSH010000957 DBNGSH010000000
VERSION     DBNGSH010000957.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3863539"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="AC16CY_21895"
     CDS             <1..567
                     /locus_tag="AC16CY_21895"
                     /inference="COORDINATES: protein motif:HMM:NF015961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3356520.1"
                     /translation="GLHHFDAIHASGRQPVMAFWHGRILPATFYFRRRGIVVITSENF
                     DGEWIARIIERFGYGTARGSTTRGGRKAILQMVRDMKKGKPTGFTLDGPRGPARVAQP
                     GAVWLASATGNPLLPFHLEASSYWSARSWDRTQVPKPFSTVALVVGQPMEVGKDASDE
                     ALEQARLEVERALAALESRAQALLGHRA"
     gene            complement(578..814)
                     /locus_tag="AC16CY_21900"
                     /pseudo
     CDS             complement(578..814)
                     /locus_tag="AC16CY_21900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
     gene            complement(849..1181)
                     /locus_tag="AC16CY_21905"
     CDS             complement(849..1181)
                     /locus_tag="AC16CY_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356521.1"
                     /translation="MDTCSACGEKLPAGATKCPLCLVAVPQNEDDDVYDLSATDETIP
                     AEWEGGAVYTLEFPARCPHCRELVRSLRVLRLKRSQVSFTSPLPRGGRAIVCSACQGI
                     ISADVSTL"
     gene            1247..2164
                     /locus_tag="AC16CY_21910"
     CDS             1247..2164
                     /locus_tag="AC16CY_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356522.1"
                     /translation="MGRYLARRLALTVPVLLGVATLVFSLIHLIPGDPAQAMLGATAA
                     DADVAQLRRQLGLDRPLIVQYVSFLQGLLFGDLGTSLRTGTPVTVELLGRIPATLELA
                     AAAMLVALAVSLPLGILAAIRRGGLVDHLAMTVSLAGACLPNFWLGPLLAIVFAVELG
                     WLPVSGRGRVDHLVLPALSLGAALAAVLARMTRASLAEELREPYVTAARARGASPSRA
                     VLVHAFRNSLIPVVTVVGLQFGAVLTGAVITETIFAWPGIGRLLIQSIGFRDYPMVQG
                     CILLIATTYAAMILLTDLLYVALDPRIRY"
     gene            2164..2955
                     /locus_tag="AC16CY_21915"
     CDS             2164..2955
                     /locus_tag="AC16CY_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356523.1"
                     /translation="MTKAGGIIVGLAVLLAAIGPWLVPFDPSAQDLSMRLEGPTALHW
                     FGLDELGRDILARLIFGARVSLLVGLVVVSISAAAGTVLGSIAGYYGGLVDDAISRVI
                     DILLAFPGLLLAIAVVAVLGPSLTHVVFALSLIGWVGYARLVRGQVLRTRELAYVEAA
                     VALGASTPRILMRHVVPAALPALTVQVTLGLGGVILSEAALSFLGLGVQPPTPSWGTM
                     LNYGRAHLLDAPHLTIFPGLAIAVLVLGFSFLGDGLRDSLTSDTS"
     gene            complement(2974..4098)
                     /locus_tag="AC16CY_21920"
     CDS             complement(2974..4098)
                     /locus_tag="AC16CY_21920"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356524.1"
                     /translation="MNRSIVAAVGVTALVTSVPLVWLNARQEGEYRRFIAAGDAAIAR
                     DQTFEAIEAFTGALTLKPDSTIARLKRGDTYRRRGEFASAVRDLAEASALDATSPRPL
                     ELLGDARGAMGQNEQAARDYERFLELDDRAPRVVYKLGLAYYRAGNLPRAVETLQRAV
                     SLDQRFAEAHYMLGLCLRDTEPAQAERALLRAVEVDPAFTAAREELVALYTTSNPLKA
                     LEQLEALSTLEPSRPERQVRVGLAQASLGRRDAAVLTLSRAADRHPDAAIVYAALGRV
                     WLEVAESQGDRIALSKAIEALAPAATWPTATSSTLALYGRALYLSGDPAAAERALRRA
                     TLQFPVDPSAFAYLADAAERLGHTQAARDASASHAALVAD"
     gene            4257..4859
                     /locus_tag="AC16CY_21925"
     CDS             4257..4859
                     /locus_tag="AC16CY_21925"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3356525.1"
                     /translation="MKHAGAIGWSDVDTAEAALIERCNTGDERACADLVNTHQRMVYT
                     LAIHLLGDPDEALDLSQEVFLRVFRTLSSFRGHSALRTWIYRIVINQARNRQRWWRRR
                     HRSSQVSLDDHLKQFGEIESKSEVLPDRLLASKETSALIWQALDRLPFDQRTALILRE
                     VDGLRYDEIAYSLGIAVGTVKSRLTRARQALRAELLGLHA"
     gene            4856..5803
                     /locus_tag="AC16CY_21930"
     CDS             4856..5803
                     /locus_tag="AC16CY_21930"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3356526.1"
                     /translation="MNTTPYIGCDYAQDRLESFVDGELSVEEQVAVESHVRWCQTCSA
                     RIEDLNLIGASVRAGSQALQGTGHSEPDLNAVTSSVLMRMDAERGQSFLVRVREQFSD
                     MRLLWPALGATVAVVLCASVAAEVLKRALIEYPESVAMRLDAMANPGSEMNPLRPDNN
                     AYVHPFFGKFVDSDRAGGISMPRVLDDGFFEGIEDREATFTMATVVGRDGRIANAELL
                     VSERNGSAERALHASDVAVLDAVRQSRLTPAQTPVGRAVAVNMVWLFVVTTVQTPDAQ
                     PAGRAAPVTARARRLPAVDAVAPVPLNQRSARTSHTPTA"
     gene            complement(5835..>6380)
                     /locus_tag="AC16CY_21935"
     CDS             complement(5835..>6380)
                     /locus_tag="AC16CY_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3356527.1"
                     /translation="YEKGLLTFVWRAEDDNGDDLSYDIFYRQEGDTSWKLLKRAVAEE
                     ILVWDTTSVPNGRYTVRVVASDARSNSPSTALNGSRESASFEVDNVPPVITVTASRRE
                     NGRAVITLDVRDAHSSVQKVDYSLDGDRWQAIYPRDGIADSRFEQFELILEGEAASRG
                     VILRATDALNNTSSVRGETGK"
BASE COUNT          945 a         2121 c         2197 g         1117 t
ORIGIN      
        1 ggccttcacc acttcgacgc catccatgcg tcgggccgcc agcccgtgat ggcgttctgg
       61 cacggccgca tccttcccgc caccttctac ttccgccgtc gcggcatcgt ggtcatcacg
      121 agcgagaact tcgacggcga atggatcgcg cgcatcatcg agcggttcgg gtacggcacc
      181 gcccgcggct cgacgacgcg aggcggacgg aaagccatcc tgcagatggt gcgtgacatg
      241 aagaaaggaa agccgaccgg attcaccctg gacgggccac gcgggccggc gcgcgtcgcg
      301 cagccgggcg cggtgtggct cgcgtcggcg accggcaatc ctctcctccc gtttcacctc
      361 gaggcgtcgt cgtactggag cgcgcgcagc tgggatcgaa cgcaggtgcc gaagccgttc
      421 agtacggtgg cgctggtggt cgggcaaccg atggaggtgg gaaaggacgc ctccgacgag
      481 gcgctggaac aggccaggct cgaggtcgaa cgcgcgctcg ccgccctcga gtcgcgcgcg
      541 caggccctgc tcggtcatcg tgcgtagcgg gttcagatca cggcacctgc actccgttct
      601 cgttgtgata gtgcccgtgg acgggatccc agacacgggt tccgccggag cgtccttcgc
      661 ggttgaacga tgccacgccg gcggcaattg cggcgagcac ggcggcgccc acgacgatca
      721 tcagtacccg tgccgagcgc ctctcgttcg acttggactc gtggcagtgc ttgtacttct
      781 tgccgctgcc gcaggggcac ggctcgtttc gtccaggttt ggccattcag aacctccgac
      841 tctgcggctt agagcgtcga tacgtccgcc gatatgatcc cctgacacgc cgagcagacg
      901 atggcccggc ccccgcgcgg caacggcgac gtgaagctga cctgcgaccg cttcaggcgc
      961 agtacccgca ggctgcgaac cagctcgcgg cagtgcgggc accgggcagg gaattcgagc
     1021 gtgtacacgg cgccgccttc ccactccgcc gggatggtct catcagtcgc cgaaaggtcg
     1081 tagacgtcgt cgtcctcgtt ctgcggcacc gcgacgaggc aaagcgggca cttggtggcg
     1141 ccggccggca gtttctcgcc gcacgcgctg catgtgtcca tgcggggatt gtagcgggac
     1201 tcggacgtcc ttgcgccgaa ttctccgaag cggaaagaat ggcgccgtgg ggaggtatct
     1261 cgcgcggcgg ctcgcgctta ccgtgcccgt cctgctcgga gtggcgacgc tggtgttctc
     1321 gctgattcac ctgattcccg gagacccggc gcaggcgatg ctcggcgcga cggccgccga
     1381 tgccgacgtc gcgcagctcc gccggcaact gggcctcgac cggccgctca tcgtgcagta
     1441 cgtctcgttt ctgcagggcc tgctgttcgg tgatctcggt acctcgctga gaacggggac
     1501 gccggtgacc gtcgagctcc tgggacgcat accggcgacg ctggagctcg cggccgcggc
     1561 gatgctcgtt gccctggccg tgtcgcttcc gctgggcatc ctggccgcaa tccggcgagg
     1621 aggcctcgta gaccatctcg ccatgaccgt gtctctggcc ggcgcctgcc tgcccaattt
     1681 ctggctcgga ccgctgctcg ccatcgtctt cgccgtggag ctcggatggc tcccggtgtc
     1741 gggacgtgga cgcgtggatc atctcgtgct gcctgcgttg tcgctcggcg cggcgctggc
     1801 agccgtcctc gcgcgcatga cgcgggcgtc gctggccgaa gagctccgcg aaccgtacgt
     1861 cacggcggct cgtgccaggg gagcctcgcc cagccgtgcg gtgctcgtcc acgcattccg
     1921 gaacagcttg attcccgtcg tgacggtggt cggcctgcag ttcggagccg tgctcacggg
     1981 tgcggtcatc acggagacaa tctttgcgtg gcccggcatc gggcggctgc tcatccaatc
     2041 gatcggcttt cgcgactacc cgatggttca aggctgcatc ctgctcatcg cgacgaccta
     2101 cgccgcgatg atcctcctga ctgacctgct gtacgtcgct ctcgatcccc ggatccggta
     2161 ctgatgacga aggcgggtgg catcatcgtg ggcctcgcgg ttcttctggc ggccatcgga
     2221 ccgtggctgg tgccgttcga tccctcggcg caggacctgt cgatgcggct tgaagggccg
     2281 acggcacttc actggttcgg actcgacgaa ctgggcaggg acattctcgc gaggctaatc
     2341 ttcggcgcgc gcgtctcatt gctggtcggc ctggtcgtcg tctcgatttc cgcagcggcg
     2401 ggcacggtgc tgggctcgat cgcaggctac tacggcggtc tcgtagacga tgcgatcagc
     2461 cgggtcatcg acatccttct ggcgttcccg ggcctgctgc tcgcaattgc cgtcgttgcg
     2521 gtcctcggac cgagcctgac ccacgtcgtc tttgccctgt cgctcatcgg atgggtcggc
     2581 tatgcgcgcc tcgttcgcgg gcaggtgctg cgcacgcgcg agctcgcgta cgtggaggcg
     2641 gcagtggcgc tgggtgcgtc cacgccgcgg atcctgatgc gccacgtcgt gcccgccgct
     2701 ctcccggcgc tgacggtcca agtcacgctc ggcctgggag gggtgatcct ctccgaagcc
     2761 gccctgagct ttctgggcct gggtgttcag ccgccgactc cgagctgggg gacgatgctg
     2821 aactacggcc gcgcgcatct gctcgacgca ccccacctga cgatctttcc cggccttgca
     2881 atcgccgtcc tggtgcttgg cttcagtttt ctcggggacg ggctgcggga ttcgctgacg
     2941 agcgacacga gctagggagc cgtggcgggc cgttcagtcg gcgaccagag ccgcgtgact
     3001 cgccgatgcg tctcgcgccg cctgcgtgtg tccgagccgt tcggccgcgt cggcgaggta
     3061 cgcgaaggcg gacgggtcca cgggaaactg cagcgtcgct cgtcgcagcg ctctttccgc
     3121 cgccgcggga tcgccggaca aatacagcgc ccggccgtag agcgcgagag tgctactggt
     3181 cgcggtcggc caggttgccg ccggggcgag tgcctcgatc gccttactca aggcgatccg
     3241 gtcgccttga gactccgcga cctcgagcca cacacgaccc agcgctgcat agacgatcgc
     3301 ggcgtccgga tggcggtcgg cggcccggct gagggtgagg acggcggcat cccgccggcc
     3361 gaggctcgcc tgggccagtc caacgcggac ctgtcgctct gggcgcgaag gctcgagagt
     3421 cgacaaggcc tcgagctgtt cgagcgcctt cagcgggttc gaggtcgtgt agagcgcgac
     3481 cagctcttcg cgcgcggccg tgaatgcggg atcgacctca acggcgcgca gcaaggcccg
     3541 ctcggcctgc gccggctccg tgtcccggag acagagtccg agcatgtaat gggcctcggc
     3601 gaaccgctgg tccaggctga ccgcgcgttg cagggtctcg acggccctcg ggagattgcc
     3661 cgcgcgatag taggccaggc cgagcttgta gacgacgcgc ggcgcacggt cgtcgagttc
     3721 gaggaatcgc tcgtaatcac gcgcggcctg ctcgttctgt cccatcgcgc cgcgcgcatc
     3781 gcccagaagc tcgagaggcc ggggggaggt cgcgtcgagg gcgctggcct cggcaagatc
     3841 gcggaccgcg gacgcgaact cgccccttcg ccgataggtg tcaccgcgct tcagccgcgc
     3901 gatcgtggaa tccggcttca gcgtcagcgc tccggtgaac gcctcaatcg cctcgaaggt
     3961 ctgatcccgg gcgatggccg cgtcgcccgc cgcaatgaaa cgcctgtatt caccctcctg
     4021 ccgcgcgttc agccagacca gcgggacgga cgtgaccagg gcggtcaccc ccacggcggc
     4081 gacaatggag cgattcatcg gtgtcgatgg taacgccaat cagggcaggg ggcagggggc
     4141 agggggcagg gggcaggggg caggcggcag gcggcaggga accccggccg tccggcgggg
     4201 tctaatgggc caaggacggt gctaaactca tgggaacacc ggtttccgga ggtttggtga
     4261 agcacgctgg cgctatcggc tggtctgacg tcgatacggc ggaagccgca ctcatcgagc
     4321 gttgcaatac gggcgacgaa cgcgcctgcg ccgacctagt caatacccac cagcggatgg
     4381 tgtacaccct ggccattcat cttctcgggg acccggacga ggcccttgac ctgtcccagg
     4441 aggtcttcct tcgagtcttt cggacgctct ccagtttccg gggccactcc gccctgcgaa
     4501 cctggatcta ccggatcgtc atcaaccagg cgcgcaaccg gcagcggtgg tggaggcggc
     4561 gtcatcgctc cagccaggtg tcgctcgacg atcacctgaa gcagtttggc gagattgagt
     4621 ccaagagcga ggtgcttccg gatcggctgc tggccagcaa ggagacatcg gcgctcatct
     4681 ggcaggcgct cgatcgcctt ccgttcgacc agcgcacggc actgatcctt cgcgaggtgg
     4741 acggcctgcg ctacgacgag atcgcctatt cgctgggcat cgcggtcggc acggtgaagt
     4801 cgcgtctgac gcgcgcgcgg caggcgctgc gggcggagct tctggggttg cacgcatgaa
     4861 cacgaccccg tacatcggct gcgactacgc gcaggatcgg ctcgagagct tcgtggacgg
     4921 cgagctgtca gtcgaggagc aggtggctgt tgaaagccac gtgcgatggt gccagacctg
     4981 ctcggcccgc atcgaagacc tgaacctgat cggggcgtcg gttcgcgcgg gttcgcaggc
     5041 gcttcagggc acgggccatt ccgagccgga cctcaacgcc gtgacgtcga gcgtgctgat
     5101 gcggatggac gccgagcgag gacagtcgtt cctcgtgcgc gtccgcgagc agttctccga
     5161 catgcggctc ttgtggccgg ccctcggcgc gaccgtggcc gtcgtcctgt gcgccagcgt
     5221 ggccgccgaa gtgttgaagc gggccttaat cgagtacccc gagtcggtgg ccatgaggct
     5281 cgacgcgatg gccaaccccg ggtccgagat gaacccgttg agaccggaca acaacgccta
     5341 cgtccatccg ttcttcggaa aattcgtgga cagcgatcgg gccggcggga tttcgatgcc
     5401 ccgtgtgctc gacgacggct tcttcgaggg gatcgaagac cgcgaggcga cgttcacgat
     5461 ggcgaccgtg gtcggccggg atggccgcat tgccaacgcc gagttgctcg tgtccgaacg
     5521 caacggctcg gccgagagag cgctgcacgc cagcgacgtg gccgtgctcg acgcggtacg
     5581 ccagtcgaga ttgacgccgg cccaaacgcc cgtcggccgg gccgttgccg tcaacatggt
     5641 gtggctgttc gtggtgacca ccgttcaaac gcctgacgcg cagccggccg gccgtgccgc
     5701 gccggtcacc gcgcgcgccc ggaggctgcc ggcggttgac gccgtggcgc cggtcccgct
     5761 caaccagcga tccgcgcgga ccagccatac gccgacggcc tgacggccgt ctcacttcac
     5821 cgcgagtcct ggagttactt ccctgtttct ccgcgtacgc tcgacgtgtt gttgagcgcg
     5881 tcggttgcgc gcaggatgac gccgcgcgag gcggcttcgc cctcgaggat caactcgaac
     5941 tgctcgaagc gcgagtcggc gatcccgtcc cgggggtaga tcgcctgcca cctgtcgccg
     6001 tcgagggagt agtccacctt ctgcacggac gagtgcgcgt ccctgacatc cagcgtgatg
     6061 acggcccgcc cgttctcgcg gcgggaggcg gtgacggtga tgacgggcgg aacgttgtcg
     6121 acctcgaacg aggcgctctc ccggctcccg ttgagcgccg tggagggaga attcgaccgc
     6181 gcatcggagg ccacgacgcg gaccgtgtag cgcccgttgg gtaccgacgt cgtgtcccag
     6241 acgagaatct cttcggcgac cgcgcgcttg agcagcttcc aggatgtatc gccctcctgg
     6301 cgatagaaga tgtcgtagct gagatcgtcg ccgttgtcgt cttcagcccg ccagacgaac
     6361 gtcagcaatc ccttctcgta
//
LOCUS       DBNGSH010000958         2119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3694600, whole genome shotgun sequence.
ACCESSION   DBNGSH010000958 DBNGSH010000000
VERSION     DBNGSH010000958.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3694600"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="AC16CY_21940"
     CDS             <1..942
                     /locus_tag="AC16CY_21940"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3356528.1"
                     /translation="DYWQRHGLSREARVLGHARTVAAWGSRLAPLSNWVAASRAVRML
                     NERLLGLDRRRRLPRFRRTTLSSLVRAPKDHADTLLFNDTFTNYYDPEIGVAAIRVLE
                     AAGRRTVLASNQCCGRPQISKGLLGEARDLASRNADGLYQDAAAGRPIVFCEPSCLSA
                     VREDAPALLRGEARRRAETVASASVLFEEFTSTIAPRLPLRPGPASILLHGHCHQKSM
                     SLVPSSISLLARIPGARVVDLDAGCCGMAGSFGYAREHYDLSRAIGERKLFPAVRSKA
                     ADAVVVAAGTSCRHQIADFTGATAIHPAVLLAELLEG"
     gene            942..>2119
                     /locus_tag="AC16CY_21945"
     CDS             942..>2119
                     /locus_tag="AC16CY_21945"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3356529.1"
                     /translation="MPLAWLSLGALVVAMIVSCTTTMNVGVLALALAWIVGVYLGGMS
                     LGDVLSGFPVQLFLTLTGVTLLFTQAQLNGTLDRVAHAAVRVCRGNAGLIPVMFFGLG
                     CVIASLGPGNVATAAMLAPMAMAVAARASIPPFLMAIMVGNGAQSGALSPVAPTGIIV
                     TGLMDKIGLGGYEIRTYAANLAAHAIIAFGGYLLLGGVRLFRHRYSGGESADQTDRLN
                     AKHWLTLAIVGGVLGTVLFLNANIGMAAFTGAVVLAALRLADHEQAIRKMPWIPILMV
                     SGVSVLVALLEKTGGLDLFTTILARFATPGTLTGVIAFVTGVISVYSSTSGVVLPAFL
                     PTVPGLIARLGGDPFSVASSMNIGAHLVDMSPLSTTGAMCIAGITDVSQVRPTYNKLL
                     A"
BASE COUNT          288 a          762 c          696 g          373 t
ORIGIN      
        1 gactactggc agcggcacgg cctgtcgcga gaggcccgcg tgctcggcca cgcgcgaacg
       61 gtcgcggcct ggggcagccg tctggcgccg ctctcgaact gggtggcggc aagccgcgcc
      121 gtccgaatgt tgaacgagcg gctgctcggc ctcgatcgac gccggcggct tcccaggttc
      181 cggcggacga cgttgagcag tctcgtccgc gcgccaaaag accacgcgga cacactgctc
      241 ttcaacgaca cgttcaccaa ctactacgac cccgagatcg gcgtcgccgc gatacgggtg
      301 ctcgaggccg ccgggcgtcg caccgtcctc gcatccaacc agtgctgcgg ccgtccgcag
      361 atctcgaagg ggctgctcgg agaggcccgc gaccttgctt ccaggaatgc cgacgggctg
      421 tatcaggacg cggcggccgg ccggccgatc gtgttctgcg agccaagctg cctgtcggcc
      481 gtacgcgagg acgccccggc cctgctgcgc ggcgaggcgc gccgtcgcgc ggagacagtc
      541 gcctccgcca gcgttctgtt cgaggagttc acctccacga ttgccccgcg gctgccgctc
      601 aggcccggac ccgcgtcgat cctgctgcac ggccattgtc atcagaagtc gatgagcctg
      661 gtcccctcgt cgatctcgct cctggctcgc atccccggcg cccgcgtggt tgacctcgat
      721 gccggctgct gcggcatggc gggctccttc gggtacgcgc gcgagcatta cgatctctcg
      781 cgagcgatcg gcgaacgaaa actctttccg gccgttcgca gcaaggcagc cgatgccgtc
      841 gtcgtcgcgg ccggcacctc gtgccggcat cagatcgccg atttcaccgg ggcgacggca
      901 atccatccgg cggtgctgct ggccgaattg ttggagggct gatgcctctt gcctggttgt
      961 ctctcggcgc cctggtcgtg gcgatgatcg tcagctgcac gaccaccatg aacgtcggcg
     1021 tgctcgcgct cgccctcgcc tggatcgtcg gcgtctatct cggagggatg agcctgggtg
     1081 acgtgctgag cggcttcccg gtgcagctgt tcctcacgct gaccggcgtc acattgctgt
     1141 tcacccaggc acagctgaac ggcacgctcg atcgcgtggc gcacgccgcg gtgcgcgtct
     1201 gccgcggcaa cgccgggctc attcccgtga tgttcttcgg gctcggctgc gtgatcgcct
     1261 cgctcgggcc cggtaacgtg gcgaccgccg cgatgctcgc gccgatggcc atggccgtcg
     1321 cggcgcgcgc ctcgattcca ccgttcctca tggccatcat ggtgggcaac ggggcccagt
     1381 cgggcgcgct ctcgccggtg gcgccgaccg ggatcatcgt caccggcctg atggacaaga
     1441 tcggcctcgg gggctacgag attcgcacgt acgccgccaa cctcgccgcg cacgccatca
     1501 tcgcgttcgg cgggtacctc ctgctcggag gcgtccggct gttcaggcac cgctacagcg
     1561 gcggcgagtc cgccgatcaa acggaccggc tcaacgcgaa gcactggctc acgctcgcga
     1621 tcgtcggcgg cgtgctcggc accgtgctct tcctgaacgc caacatcggc atggccgcgt
     1681 tcaccggcgc ggtcgtcctc gcggcgctgc ggctggcaga ccacgagcag gccatccgca
     1741 agatgccctg gattcccatc ctgatggtga gcggcgtcag cgtcctggtg gcgctgctcg
     1801 agaagaccgg cggcctcgat ctgttcacga cgattctcgc gcgattcgcc acgcccggga
     1861 cgctgactgg tgtcatcgcc ttcgtgaccg gcgtcatctc cgtctacagc agtacgtcgg
     1921 gcgtcgtgct gccggccttc ttgcccaccg tccctggact catcgcgcgt ctcggcggcg
     1981 atcccttcag cgtcgcctcg tcgatgaaca tcggggcgca cctcgtggac atgtcgccgc
     2041 tgtccacgac cggcgccatg tgcatcgcgg ggatcacgga cgtctctcag gtgcggccga
     2101 cctacaacaa gctcctcgc
//
LOCUS       DBNGSH010000959         5353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3186260, whole genome shotgun sequence.
ACCESSION   DBNGSH010000959 DBNGSH010000000
VERSION     DBNGSH010000959.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3186260"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1118)
                     /locus_tag="AC16CY_21950"
     CDS             complement(150..1118)
                     /locus_tag="AC16CY_21950"
                     /inference="COORDINATES: protein motif:HMM:NF052887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8021 domain-containing protein"
                     /protein_id="HIR3356530.1"
                     /translation="MLLGGVAAAQGTGQCDRECLVGISEAYLDALVAKDVSKLPLAGP
                     AVKYTENGQRLRLGDGLWNSVSARGTYKLHVADTTQGHIATIITMREAGTPIILVVRL
                     KVDKRRISEIESMVARSENGAKGFEALGQPREAFSRLTPAADRASRADMVRVANMYFS
                     GMQLNDGKGQYPFADDCDRIENGGRSTNAPGRGGAPRPDPKTASNYSSMWTCREQFES
                     GLLHFVSRIRDRRYVAVDEERGLVMAFVFFDHDAGASRTFKTPDGRTVTAGPVTPWTW
                     QIAELFKVEKGLLHEIEAVLERVPYGMTSGWSSWDDGMSDKIQFSR"
     gene            complement(1157..2461)
                     /gene="hmgA"
                     /locus_tag="AC16CY_21955"
     CDS             complement(1157..2461)
                     /gene="hmgA"
                     /locus_tag="AC16CY_21955"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HIR3356531.1"
                     /translation="MAATERPYLSGFGNEFASEALDGALPREQNTPQRPAYGLYIEEL
                     NGTAFTAPRGISRSTWTYRIRPSAMHKPFTPRDSRLIRSGPFNEVPPTPNQLRWKPMP
                     IPTAPTDFVDGIVTLGGNGDPTFQSGAAVHMYAANTSMKDRFFYDADGELLIVPQAGA
                     LAVHTEFGILDVPPGHIALIPRGVKFRVELPDGSARGFICENYGQHLRLPDLGPIGTF
                     GLANARDFEAPAAAFEDREGSFTVTAKFGGGLWEAEFDHSPLDIVAWRGNYVPFRYNL
                     YKFQCINTVTFDHPDPSIYCVLASPSTTPGTSNMEFGCFPPRWSVAEHTFRPPPFHRN
                     VASEFLGLLAGKYIGKGEGFGPGSASLHNCMSGHGPDAEAYERGRSAELKPQYLDDTM
                     VFLFETQLPIRPTKFALETDLLEREYYQHWQGLKKNFGLVGA"
     gene            2687..3007
                     /locus_tag="AC16CY_21960"
     CDS             2687..3007
                     /locus_tag="AC16CY_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356532.1"
                     /translation="MKTQLGELDSMVIGDRPVIRMFTHELNTVRWETRIAEGRGLELN
                     VLGPGALRRSYSFADALALVEYQVQFERQLLSNGYAVLPQSERRTGYDRRLRPRSEAD
                     RRKQ"
     gene            3021..3293
                     /locus_tag="AC16CY_21965"
     CDS             3021..3293
                     /locus_tag="AC16CY_21965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356533.1"
                     /translation="MLQIVASNDSPTQRLALARRHIETAIAHINDVAQTRVPPSIVDD
                     SAGVDELRLIVRALADIREALRASDWTPARLDSEPLAQPNQPENDW"
     gene            3353..5323
                     /locus_tag="AC16CY_21970"
     CDS             3353..5323
                     /locus_tag="AC16CY_21970"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3356534.1"
                     /translation="MRTAGVSLALVVSMALAGWTLSGRAADGDRDWPAYGGDKAGTKY
                     SPLDQINKDTIKNLRIAWRRSGMPEELRSAFPDIQASANYQHTPLVVDGLLYLSSAVG
                     AVAALDPATGRTVWHDTLPPRPDGQGLARGGPTRGIAYWTDGRDARIVTNVGANLVAL
                     NAKTGKRYADFGDNGQVDLTKGFERPITGWRWSSGPIVVRDVVVVAGVPSPATDILNE
                     RVRAPKEMPPDDVRGYDVRTGKLLWTFHVVPRKGEFGNETWLNDSWTYSGNSGAWSLL
                     SADEELGYVYLPFEEATGDYYGGTRPGNNLFAESIVCLDARTGRRVWHFQTLHHGLWD
                     YDLPAAPVLGDITVDGRRIKAVAQVSKQAYVYVFDRVTGEPVWPIVERPVPQGNVPGE
                     WYSPTQPIPSKPPAFDQQGVSDNDLIDFTPELRAEAKRIVSEYKTGPLYTPPVLLGSP
                     DGPKGLLLVPGTNGGADWGGASFDPETGILYVPSAHMPTVIALGKSLHPESTLPYVKQ
                     QTPWPFGPQGLPDPFKPPYARLVAIDLNKGDILWSVANGDGLRNHPAFKGLNLPPIGT
                     PGRNAALVTRSFVFMGEGSDAGVGLPPGGGYGGKMFRAFDKKTGAIAWEMELPAGVSN
                     APMTYQFNGKQYIVVAVSGRQHPGEFIALALP"
BASE COUNT          939 a         1756 c         1741 g          917 t
ORIGIN      
        1 ccctgccccg ccggcggccc ctgcagcgcc gacaggttcg cggccgccgc ggcaacggcg
       61 cccgcaatca caatcacgcc cagcacgtat gtgcgcttca tgaccacccc tcgcaagagt
      121 caagacgaat tagggaactc actgatattt cagcggctga attgtatctt gtccgacatc
      181 ccgtcgtccc agctgctcca gcccgacgtc atcccgtagg gaacccgttc gagcaccgcc
      241 tcgatctcgt ggagcagccc cttttcgacc ttgaaaagct cggcgatctg ccaggtccag
      301 ggcgtcaccg gaccggccgt caccgtgcgg ccgtcgggcg tcttgaacgt tcgggacgcc
      361 ccggcatcat ggtcgaagaa gacgaacgcc atcactaggc cccgctcttc gtccacggcg
      421 acgtagcggc ggtcgcggat gcggctgacg aaatgcagga gcccggactc gaactgctcg
      481 cggcacgtcc acatcgacga gtagttgctg gccgtcttcg gatcgggcct tggcgcccct
      541 ccgcgccctg gcgcgttggt ggatcgtcct ccgttctcga tgcgatcgca gtcgtcggcg
      601 aacgggtact gccccttgcc gtcgttgagc tgcatccccg agaaatacat gttggccact
      661 cgaaccatgt cggcacggga cgcacgatcg gcggccggcg tcaggcggct gaacgcctcg
      721 cgcggctgtc cgagcgcttc gaagcccttg gcgccgttct cgctgcgggc aaccatcgac
      781 tcgatttcgc tgatgcgacg cttatcgacc ttgaggcgga cgaccaggat gatgggcgtg
      841 cctgcctcgc gcatggtgat gatcgtcgcg atgtgtccct gcgtggtgtc ggcgacgtga
      901 agcttgtagg tgccgcgggc cgagacggag ttccagaggc cgtcgcccag gcgcagacgc
      961 tgcccgttct ccgtgtactt gacggccggg cccgcgagcg gaagcttcga cacgtccttg
     1021 gcaacgaggg cgtcgagata cgcctccgag atgccgacaa ggcactcgcg gtcgcactgc
     1081 ccggttccct gtgcggcggc cacaccgccg agcagcatga acgccgcgag gactacccct
     1141 ccccgaacca tggacgtcag gcccccacca gaccgaagtt cttcttcagg ccttgccagt
     1201 gctggtagta ctcgcgctcc agcagatcgg tctcgagcgc gaacttggtc ggcctgatcg
     1261 gcaactgggt ctcgaacaag aacaccatgg tgtcgtcgag atactgcggc ttgagctcgg
     1321 cgctcctgcc gcgctcgtac gcctcggcgt cgggcccatg ccccgacatg cagttgtgga
     1381 ggctcgcgct tccggggccg aatccttcgc ccttgccgat gtacttccct gccagcagcc
     1441 cgaggaactc gctcgccacg ttgcggtgaa acggcggcgg tcgaaacgtg tgctcggcca
     1501 ccgaccagcg cggcggaaaa cagccgaact ccatgttgga agtcccggga gtggtcgatg
     1561 gcgaggccag cacgcagtag atcgatggat ccggatggtc gaaggtgacc gtgttgatgc
     1621 actggaactt gtacaggttg tatctgaacg gtacgtagtt gccgcgccag gccacgatgt
     1681 cgagcggcga gtgatcgaac tcggcctccc acaggccgcc gccgaatttc gccgtcacgg
     1741 tgaagctgcc ttcgcgatcc tcgaacgcgg ccgccggcgc ctcgaagtcg cgcgcgttcg
     1801 cgaggccgaa cgtgccgatc ggaccgaggt cggggagccg caggtgctgt ccgtagttct
     1861 cgcaaatgaa cccgcgcgcc gaaccgtcgg gaagctcgac tcggaacttg actcccctcg
     1921 ggatcaacgc aatgtgcccg ggaggaacat cgaggatgcc gaactcggtg tgcacggcga
     1981 gagccccggc ctgcggcacg atgagcagct cgccgtcggc gtcgtagaag aagcggtcct
     2041 tcatcgacgt gttggccgcg tacatgtgta cggcagcgcc actctggaag gtcggatcgc
     2101 cgttcccgcc gagcgtcacg atgccatcga cgaaatcagt tggcgccgtc ggaatcggca
     2161 tcggcttcca gcgcagctgg ttcggcgttg gcggaacttc gttgaacggt ccactccgga
     2221 tcaagcggct gtctcgcgga gtaaacggtt tgtgcatcgc ggatggccga atgcgatagg
     2281 tccacgtcga ccggctgatt ccacgaggcg ccgtgaacgc cgtcccgttc agttcctcga
     2341 tgtagagtcc gtaggcgggc cgctgcggtg tgttctgctc ccgcggcaac gcgccgtcga
     2401 gcgcctccga cgcgaactcg tttccgaagc cggacaggta gggacgttcc gtcgccgcca
     2461 tggcggactg taggctaacc ctgccaccgt ggaaggtcaa ccttgggcgc cgacgcggct
     2521 ccatcggctg ggcatcctgg gccatccggc aaccgtgcgc atattcggcg cacagattgc
     2581 taacgccaac cgtggaggtt aggattctcg cgcaaactgc ggggattcac tgtcggaagg
     2641 ctctaagaag aagggaacaa agtccgtctc cacggagact gtccacgtga aaacgcagct
     2701 cggagagttg gattcgatgg tcatcggcga ccgcccagtg attcggatgt tcacacacga
     2761 gctcaacacg gttcggtggg aaacacggat cgcggaggga cggggactcg agttgaacgt
     2821 gctaggcccg ggggcgttgc ggaggtcgta ctcattcgcc gacgcactcg cactcgtgga
     2881 gtatcaggtc cagttcgagc gccagttgct gtcgaacggg tacgctgtgc tcccgcagag
     2941 cgagcggcgc acaggatacg accggcgcct gcggccgcgc tcggaagcgg accggcgcaa
     3001 gcagtgattt tcgagggagc atgctccaaa tcgtagccag caacgactcg cccactcagc
     3061 ggcttgcgct cgcgcgcagg cacatcgaga cggcgatcgc ccacatcaac gacgtcgcac
     3121 agactcgtgt gcctccgtcg atcgtggacg actccgctgg cgtggacgag ctccgtctca
     3181 tcgtccgcgc gctggccgac atcagggagg ccctccgcgc atcggactgg acgcccgcgc
     3241 gtctcgacag cgagcctctc gcccaaccga accagcccga gaacgactgg tagcaccttt
     3301 cgcaccctcg caccttcgca ccttcgcacc ttcatcctgt tacactcccc ccatgcgtac
     3361 agcaggtgtg tctctcgcac tcgttgtctc catggcattg gccggctgga cgttgtccgg
     3421 acgagcggcc gacggtgacc gggactggcc cgcatacggt ggcgacaagg cgggcacgaa
     3481 gtattcgccg ctcgaccaga tcaacaagga cacgatcaag aacctgcgga tcgcctggag
     3541 acggtcgggg atgccggaag aacttcgatc cgcctttccc gatatccagg cctcggccaa
     3601 ctatcagcac acgccgctcg tcgtggacgg cctgctctat ctgagctccg ccgtcggcgc
     3661 cgtcgcggcg ctcgacccgg cgactggacg gaccgtctgg cacgacacgc tgcctccgcg
     3721 ccccgatggc caggggctcg ccaggggcgg acccacccgg ggtatcgcct actggaccga
     3781 cggccgcgat gcgcgcatcg tcaccaacgt cggggcgaac ctggttgcgc tgaacgcgaa
     3841 gacgggcaag cgatacgccg acttcggcga caacgggcag gtggacctga cgaaggggtt
     3901 cgagcgaccg atcaccggat ggcgctggag cagcggccca atcgtcgtcc gggatgtggt
     3961 ggtggtggcc ggcgtgccgt cgccagccac tgacatcctg aacgagcgcg tacgcgcccc
     4021 caaggagatg ccgcccgacg acgtacgtgg ctacgacgtg cgcacgggca agctgctgtg
     4081 gacgtttcac gtcgtgccgc gcaagggcga gttcggcaac gagacgtggc tcaacgactc
     4141 atggacctat tcgggcaaca gcggcgcatg gtcgctcctg agcgcggacg aagagctcgg
     4201 ctacgtgtac ttgccgttcg aggaggccac cggcgattac tatggcggca ctcggcccgg
     4261 caacaacctg tttgccgaat cgatcgtctg cctcgatgcg cgaaccggcc ggcgtgtctg
     4321 gcatttccag actcttcatc acgggctgtg ggactacgac ctgcccgcgg cgccggtgct
     4381 cggcgacatc accgttgacg gccgccgaat caaggccgtg gcgcaggtgt cgaagcaggc
     4441 ctacgtctat gtgttcgatc gggtcacggg cgaacccgtc tggccgatcg tcgagcgccc
     4501 ggtgccgcaa ggcaacgtcc cgggtgagtg gtattcgccg acgcagccga tcccgtccaa
     4561 gcctccggcc ttcgaccagc aaggcgtgag cgacaacgat ctcatcgact tcacgcccga
     4621 gctgagagcg gaggcgaaga ggatcgtgag cgaatacaag accggcccgt tgtacacgcc
     4681 gccggtcctg cttggcagtc ccgatggccc caaggggttg ctgctggtcc cggggaccaa
     4741 cgggggcgcg gactggggcg gcgcgtcctt cgatcccgag accggcatcc tgtatgtgcc
     4801 gtcggcccac atgccgactg tgatcgcgct cggcaagtcg ctccacccgg agtcgacgct
     4861 gccgtacgtg aagcagcaga cgccgtggcc gtttggtccg cagggacttc ctgatccgtt
     4921 caagccgccg tacgcgcggc tggtcgcgat cgatctgaac aagggcgaca tcctgtggtc
     4981 cgtcgccaac ggcgacggtc tgcgcaatca cccggccttc aaggggttga accttccacc
     5041 gatcggcacg cctggccgca acgcggcgct cgtaaccagg tcgttcgtct tcatgggcga
     5101 gggaagcgac gccggcgtcg gcctgccgcc cggaggcggg tacggcggaa agatgttccg
     5161 agccttcgac aaaaagaccg gggcgatcgc atgggaaatg gagctgccgg ccggagtgag
     5221 caacgccccg atgacctatc agttcaacgg caagcagtac atcgtcgtcg cggtttcagg
     5281 gcgccagcac cctggcgaat tcatcgccct ggctctgccg tgataaatgg ggtcgggagt
     5341 catttcttga gtg
//
LOCUS       DBNGSH010000960         8382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3525343, whole genome shotgun sequence.
ACCESSION   DBNGSH010000960 DBNGSH010000000
VERSION     DBNGSH010000960.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3525343"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16CY_21975"
     CDS             <1..291
                     /locus_tag="AC16CY_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="band-7 C-terminal domain-containing protein"
                     /protein_id="HIR3356535.1"
                     /translation="EAEGQAAAILAVATATAEGLRQVADAMRTEGGLEAVQLRVAEQY
                     IAQFGELAKSTNTLVLPANAADVGSMIALAMKVITGADARGGTPPPLRPRDV"
     gene            366..1133
                     /locus_tag="AC16CY_21980"
     CDS             366..1133
                     /locus_tag="AC16CY_21980"
                     /inference="COORDINATES: protein motif:HMM:NF046841.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6982 domain-containing protein"
                     /protein_id="HIR3356536.1"
                     /translation="MATNVASSIDRREHRRFTALALRGLHGARVKYGQDITVLNLSAG
                     GVWFETTSQLSPDSTIVLEFLGPGHSTLIPSRVMRCQPAPATEHGARIKSACAFKRLL
                     RVKDLVTGPTLPIDAVLSIEDAGQWQPVVGSYGDGRLVRGYTSDFSPSKSHLRIAPAR
                     SAAEAQPVFLNDLDALFFMRDDPVLANGAGAELARESASPYGRRVAMALPGGEELVGS
                     TLDYKRGHSCFIHPSENDFGVERVFVTPKGVRKVRLL"
     gene            1223..1642
                     /locus_tag="AC16CY_21985"
     CDS             1223..1642
                     /locus_tag="AC16CY_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3356537.1"
                     /translation="MGLPRLSRLELQIMETLWSHGATSVREIQEAFPEKARPAYTTVQ
                     TMVYRLEAKGAVRRSKKIGNAHVFEAVVSRNAAQRRLIDDLLGLFGGSSQPVVAHLIE
                     SGSLTLEDVREAERMLKRLQAEKTASSKVKGRSRRLT"
     gene            2017..2883
                     /locus_tag="AC16CY_21990"
     CDS             2017..2883
                     /locus_tag="AC16CY_21990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3356538.1"
                     /translation="MTKRTLSCAAVAAGVLSMVTGQGPARAQAPAGNPAFEVASVRPN
                     KSGDGRVMLGVQPGGRFTATNVPLKMLLRQAYNVQDFQVVGGPEWLASDRFDVVAKAA
                     EEGINFETMRPLVRSLLADRFKLKVHSETRQMPIYALVKARSDGTLGPSMKPSSADCA
                     ALMGRRGGGPPPALPPPGQPADCSFIIGIGKLSVGGMPVEQLARALSPMVGRVVLDKT
                     GLTGNYTYELTYSPEQLGGQPLLNGGVPAGDPSSPSIFTALQEQLGLKLDSGRGPVEV
                     LVVDSAEQPTAD"
     gene            2906..5992
                     /locus_tag="AC16CY_21995"
     CDS             2906..5992
                     /locus_tag="AC16CY_21995"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3356539.1"
                     /translation="MKRAIAYVLVLATAIVNVPVSLAAASEHYGQVTVAGVAIPGATV
                     TATRGDHRLVVYTDAQGIFRLADVEDGVWAISVEMRGFSTTSREVTTGPDAPPSMWEL
                     TLLPFEEVAKTIAPPAPPPVRTPDKPGAGQPERQQSGARTPVAVPQTGFQRAGVTAPR
                     NPAPAPAPAAADDPPADAAAAADGFLINGSVNNGAASPFAQAAAFGNNRRRPGSLYNG
                     MVGAEFSNSFWDAQPYSLTGRPTLQPDYYNVNLLSTFGGPVKLPGVQNRLNFFVGFQR
                     AVRDDASTQPALMPSVRERRGDFSETRDASGNLVQIRDPLTGLPFPGNVIPPDRISPQ
                     AASLLQYYPAATAGATGRYNYEALTLRATRSISVQTRLTQVINNRNQIQGSVSYQHSG
                     TDSTTLFGFEDENVAPAADVQVNWSHRFGLLASSRVRYQLTQQRSEAIPYFAYRTNVS
                     GEAGITGNNQEPANWGPPALSFASGIAGLSDGLPGLDRNLTNAVGGDFLFSRGRHSFT
                     IGGDVRHNQVDIDSQQDPRGRFSFTGAGSGFDFADFMLGFPSTSGIAYGNADKYFRGN
                     SYDAYITDDWRVGPSLTVTAGVRWDYESPYTELQGRLVNLDVAPDFSAIAPVVATDPT
                     GALTGESYPSSLVRPDLAGIQPRLAVAWRPVPGSSIVIRAGYGIYRNTNLYRSLTNNM
                     AQQPPLSNALSVSSTPDNRLTLANGFTTPEGLTANTFAVDPDFRNGSAHNWQASIQRD
                     LPASLTINASYLGSRGTGLIREFLPNTYPRGGVNPCAACPAGFVYLTSDGTSSRHAGQ
                     IQVRRRLRSGFTATVQYTLARAFDNAASYQGANLSGTAFAQDWLNLDAEYAPSSFDQR
                     HLMTVQFQYSTGVGVAGGALIDGWQGALLKGWTLIGQMTTGSGLPLTPYFLTPTAGTG
                     YSGATRASLTGESTAAPEGHYLNPAAYRAPDPGSWGDAGRNSGTGPAQFQMNAGVQRT
                     FQLGGRYNMDFRIDATNVLNAVTYSSVNAVVGSPQFGLPESANQMRRVTSSLRLRF"
     gene            5992..8103
                     /locus_tag="AC16CY_22000"
     CDS             5992..8103
                     /locus_tag="AC16CY_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3356540.1"
                     /translation="MKKNVVVWTLLLAALGAQSAAQQQPQRETPQATFRSTTRLVVQT
                     VTVKDADGKVVEGLTAKDFTVTEDGQPQEIAFVEFQRMQNTPLTPDAVPTTIAQAPLT
                     QADPPVQAVTPTTLTQIAAPPSGDIRYQDRRLLVFYFDASSMPLPDQVRAYSNALKYI
                     DTQMTPSDLVAIMAFQNGAVSVKTDFTDDRNKLREVIQILTYGDDQNNDGIPDHPEGT
                     AFGQNDAEFNIFNTDRQLAALQTALAMLRPLPELKTLIYFASGLRLNGADNEAQLRAT
                     VNAAIRANVTLNPVDARGLVATPPLGDATRRSPGGVAMFSGKLAEAMVTNFARSQDTL
                     YALAKDTGGTAMFDYNDLSLGIQRAAQAVSDYYILGYYSTHVENDGKFRRVRISLSGG
                     RTGEVAHRQGYFADKSFARFNDSDKERQLEDAMMLGDPVTDITIAMEVNFFKLNSAEY
                     FVPVAVKIPGSELELARRRGAARTLIDFIGEVKDGYGYTIQNIRDRLDIKLSEETATQ
                     LATRPIQYETGFTLLPGKHVIKILARDSTTGRIGTYQASFTIPNLMKEAQRLPISSVV
                     LSSQRVPMTDALHTVQTGAAQLTHPLVFDGQKLFPSVTRVFSRGREMFVYLQAYEQAA
                     TSTQPLVAFVSFFRDGHKVLETEPLGITDGLDPKSKAVPIRLSIPLEGLAPGRYDCQV
                     TVLEPTGRRAAFWQAPIAVVP"
     gene            8195..>8382
                     /locus_tag="AC16CY_22005"
     CDS             8195..>8382
                     /locus_tag="AC16CY_22005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356541.1"
                     /translation="MTLLRRRCARFVAVAIVVAATASLAAQATAPSGKSFLWKIQAGS
                     NVVYLAGSVHALNADAYPL"
BASE COUNT         1472 a         2882 c         2706 g         1322 t
ORIGIN      
        1 gaagccgagg gtcaggcggc cgccattctg gccgtggcca ccgcgacggc cgaaggtctc
       61 cgtcaagtgg ccgacgcgat gcggaccgag ggcggcctgg aagcggtgca gctccgggtc
      121 gccgagcagt acatcgccca gttcggcgag ctcgccaagt ccaccaacac gctcgtgctt
      181 ccagcgaacg ccgccgatgt gggctcgatg atcgccctgg ccatgaaggt gatcacgggc
      241 gccgacgccc gaggcggaac gccgccgccg ctgcggcccc gcgacgtctg acagccgctg
      301 cccggcaccg aacggccgag attgccaatc gccgcccgtc cgaggcagag tattgcgctg
      361 ggcccgtggc aacgaacgtc gcttcgtcca tcgatcggcg tgaacatcgg cgattcacgg
      421 cactcgcgct gcgcgggctg catggcgcgc gtgtgaagta cggtcaggac atcaccgtcc
      481 tcaacctgtc agcaggcgga gtctggttcg agaccaccag ccagctgtcg cccgactcca
      541 ccatcgtcct ggaattcctc ggacccggac actccaccct gattccctcc cgggtcatgc
      601 gctgccagcc cgctcccgcg accgagcacg gtgcccgcat caagagcgcg tgcgcgttca
      661 agcgcctgct gcgggtcaag gacctggtca ccgggccgac gctgcccatc gacgctgtgc
      721 tcagcatcga agacgcgggc cagtggcagc ccgtcgtcgg ctcgtatggc gatgggcgtc
      781 tcgttcgcgg atacaccagc gacttcagcc cgtcgaaatc acacttgcgc atcgcgccgg
      841 cgcgatccgc agccgaggcg cagcccgtct tcttgaacga tctggacgcg ctcttcttca
      901 tgcgggacga ccccgtcctc gcgaacggcg ccggtgcgga gctcgcgcgc gaaagcgcgt
      961 cgccgtacgg ccgcagggtc gcgatggccc tgcccggcgg cgaggaactc gtcggctcga
     1021 cgctcgacta caagcgcggc cacagctgct tcatccatcc gtccgagaac gacttcggcg
     1081 tcgagcgcgt cttcgtgacg cccaagggcg tccgcaaggt caggcttctc taggctcggc
     1141 tcccgtcccg ggggtcccgg ggggccctct tgccactacg actacagttg tagtagatct
     1201 aaccacgcta ttaggaggcg ccgtgggatt gccgcgactg agcaggttgg agctccagat
     1261 catggagacg ctgtggagcc acggcgcgac ctcggtgcgc gaaatccagg aggccttccc
     1321 cgaaaaagcc cggcctgcgt acacgaccgt ccagacgatg gtctaccggc tcgaagcgaa
     1381 gggggccgta cggcggtcga agaagatcgg caacgcgcac gtgttcgagg ccgtcgtctc
     1441 gagaaacgcc gcccagcggc ggctcatcga cgatttgctc ggcctgttcg gcggcagcag
     1501 ccagccggtg gtcgcccatt tgatcgaatc cggcagcctg acgctcgagg acgtcaggga
     1561 agccgagcgg atgctgaagc ggctgcaggc ggagaagact gccagttcaa aggtcaaagg
     1621 tcgaagtcgg aggctcacat gattggaccc ttagccgatc acctgtggca atcgacgctg
     1681 tttgcggcgg gcgtggcgct gcgcgcactg gtcttccggc gcaaccggcg ctggatacct
     1741 ccccccggct ggtttcctgt atctcttcca ccccacggcg caagcgtgcg ccgcggggac
     1801 cccggctttg cgcgcttcgc gcgccgcgac cgttacgagg atgagcaacg agaacgtaaa
     1861 tcgccgctcg gggccgcata acggtcagcc ctggaaccat ttgccggcgt cgggatcgaa
     1921 tcgggaagac cgtccggcga gcgaaccaag cgggaaaccc gaacgtcaaa tactgcgttc
     1981 gcagacacac gggaacctgt atccccgaaa atggcaatga cgaaacgaac cctgtcgtgt
     2041 gccgccgtcg ccgccggtgt cctctcgatg gtgacgggtc aggggcccgc gcgcgcgcag
     2101 gcgcccgcgg gaaacccggc cttcgaggtc gcctccgtga gaccgaacaa gtcgggcgac
     2161 ggccgtgtca tgctcggcgt gcagccggga ggtcgattca cggcaacgaa cgtcccgctg
     2221 aagatgctgt tgaggcaggc ctacaacgtc caggactttc aggtggtcgg cggccctgaa
     2281 tggctcgcct ctgaccgctt cgacgtcgtg gccaaggccg ccgaagaggg catcaacttc
     2341 gagacgatgc gtccgctggt gcggtcgctg ctggccgatc gcttcaagct gaaggtccac
     2401 agcgagacgc ggcagatgcc gatctacgca ctcgtcaagg cccgcagtga cggcacgctc
     2461 ggcccctcca tgaaaccgtc gagcgcggat tgcgccgccc tcatgggacg gcgcggcggc
     2521 ggcccgccac cagcgctgcc tccacccggc cagcccgccg actgctcctt catcatagga
     2581 attggcaagt tgagtgtcgg tggcatgccc gtcgagcagc ttgcgcgtgc cctgtccccg
     2641 atggtgggcc gcgtcgtctt agacaagacc ggcttgacgg gaaactacac ctacgagctc
     2701 acgtattccc ccgagcagct gggtggccag ccgctgctca atggcggcgt tccggcgggc
     2761 gacccgagct ccccgtcgat cttcacggcg ctccaggagc agctcggact caagctggac
     2821 tcgggcaggg gtccggtcga agtactggtg gtagacagcg ccgagcagcc gacggcggat
     2881 tgaggaacgc ggattgagga acactgtgaa gagggcgatc gcgtacgtct tggtactcgc
     2941 aacggcaatc gtcaacgtac cggtgtcgct agcggcggcc tcggagcact acggacaggt
     3001 gacggtcgca ggcgtcgcca tccccggcgc gaccgtcacc gcgacgcggg gcgaccatcg
     3061 gctcgttgtg tacaccgacg cgcagggcat cttccggctc gcggacgtgg aggatggcgt
     3121 ctgggcaatc agcgtggaga tgagaggctt ttcaacaacg agccgcgaag tcaccacagg
     3181 tcccgacgcg ccgccgtcga tgtgggaatt gacgctgctg cccttcgagg aagtcgcaaa
     3241 gacgatcgca ccgccggctc cccctccggt tcgaaccccg gacaagccag gcgcgggcca
     3301 gccagagcgg cagcagtcgg gcgcacgcac accagttgcc gttccgcaga ccggttttca
     3361 gcgcgcgggc gtgaccgcgc cccggaaccc cgcgccggct ccggctccgg ccgccgcgga
     3421 cgacccgccg gcggacgcgg cggcggccgc cgacgggttc ctgatcaacg gcagcgtcaa
     3481 caacggcgcc gcatctccgt tcgcacaggc cgccgcgttc ggcaacaacc ggcggcggcc
     3541 gggttcgctc tacaacggaa tggtgggcgc cgagttcagc aactccttct gggacgcgca
     3601 gccgtactcg ctcacgggcc ggccgacgtt gcagcccgac tactacaacg tcaacctgct
     3661 ctcgaccttt ggcggccccg tcaagcttcc gggcgtgcag aaccgcctca acttcttcgt
     3721 tggcttccag cgcgcggtca gagacgacgc ttcgacgcag cctgcgctga tgccgtcggt
     3781 tcgcgaacgt cgcggggact tctcggagac ccgcgacgcc tccggcaatc tcgttcagat
     3841 ccgcgatccg ctgacgggcc tcccatttcc cggcaacgtc attccgcctg atcgcatcag
     3901 ccctcaagcc gcctcgctgc ttcagtacta tccggccgcg accgctggcg cgacgggccg
     3961 ctacaactac gaggcgctca cgttgagggc cacgcggagt atcagcgttc agacgcggct
     4021 gacgcaggtc atcaacaatc gcaaccagat ccagggtagc gtgtcgtatc agcacagcgg
     4081 caccgactcg acgacgctct ttgggttcga agacgagaac gtcgcgccgg cggccgacgt
     4141 gcaggtgaac tggagccatc gcttcgggct gctggcgtcc tcgcgcgttc gttatcagct
     4201 cacgcaacag aggtcggagg cgattcccta cttcgcctat cggaccaacg tctcgggtga
     4261 agcgggcatt acgggcaaca accaggagcc ggcgaactgg ggaccgccgg ccttgagctt
     4321 tgcgagcggg atcgcgggcc tgagcgacgg gctgccaggg ctcgaccgaa acctgaccaa
     4381 cgccgtgggc ggggacttcc tcttcagccg cggccgccac agtttcacga ttggcggcga
     4441 cgttcgccac aatcaggtcg atatcgactc gcagcaggat ccgcggggac gtttttcgtt
     4501 caccggcgcg ggaagcggct tcgacttcgc cgacttcatg ctcggattcc cctcgacgag
     4561 cgggatcgcc tacggcaacg ccgacaagta cttccgcggc aactcgtacg acgcgtacat
     4621 cacggacgac tggagagtcg ggccgtcgct gacggtcacg gccggcgttc ggtgggacta
     4681 cgagtcgccg tacacggaac tgcagggccg cctggtgaac ctcgacgtcg cgccggactt
     4741 ctccgcgatc gcgcccgtgg tcgccaccga cccgacgggg gcgctgacgg gcgagtcgta
     4801 cccgtcctcg ctcgtccgtc ccgacctggc aggcatccag cctcgccttg cggtggcgtg
     4861 gaggccggtg cccggctcct cgatcgtcat ccgcgccgga tacggcatct accgcaacac
     4921 caacctgtac cgctcgctca cgaacaacat ggcgcagcag ccgccgctct cgaatgcgtt
     4981 gagcgtgtcg agcacgcccg acaaccgcct gacgctggcc aacggattca cgaccccgga
     5041 aggattgacg gcaaacacgt tcgccgtcga tccggatttc cgcaacggct cggcgcacaa
     5101 ctggcaggcg tcgattcagc gcgatctgcc cgcgtcgctg accatcaacg cgtcgtatct
     5161 cggctcgaga ggcaccggcc tcatccgcga gttcctgccg aacacgtacc cgaggggcgg
     5221 cgtcaacccg tgcgcggcgt gtccggcggg cttcgtctac ctgacgtcgg acggcacctc
     5281 gtcgcgccac gcgggccaga tccaggtgcg gcggcgcctg cgcagcgggt tcacggccac
     5341 agtgcagtac acgctcgcgc gagccttcga caatgccgcc tcgtatcagg gagcgaactt
     5401 gagcggcacc gctttcgcgc aggactggct caacctcgac gccgaatacg cgccctcgag
     5461 cttcgatcag cggcacctga tgacggtgca gtttcaatac tcaacgggcg tgggcgtcgc
     5521 gggcggcgcg ctcatcgacg ggtggcaagg cgcgctgttg aaaggctgga cgctcatagg
     5581 gcaaatgacg acgggcagcg gcctgccgct cacgccgtat ttcctcacgc cgacggctgg
     5641 cacgggttac agtggcgcga cgcgtgcctc gctgaccggc gaatcgaccg cggcacccga
     5701 agggcactat ctcaacccgg cggcgtatcg cgcgcccgat ccgggaagct ggggcgacgc
     5761 ggggcgcaat tcggggaccg ggccggcgca gttccagatg aacgcggggg tccagcgcac
     5821 gttccagctg ggcggccgct acaacatgga cttccggatc gatgcgacca acgtgctgaa
     5881 tgcggtcacg tattcgagcg tcaacgccgt agtcggcagc ccgcagttcg ggctgccgga
     5941 atcggccaac cagatgcgcc gcgtgacatc gagtctgcga ttgaggttct gatgaaaaag
     6001 aatgttgtcg tctggacgct gctgctcgcg gctctgggtg cgcagagcgc cgcccagcag
     6061 cagccgcagc gggagacgcc tcaagcgacg tttcgcagca cgacgcgcct cgtggtccag
     6121 acggtcaccg tcaaggacgc agacggaaag gtcgtcgaag ggctgacagc aaaggacttc
     6181 accgttaccg aggatggcca gccgcaggag atcgccttcg tcgagttcca gcggatgcag
     6241 aacactccgc tcactccgga tgcggttccc acgacgatcg ctcaagcccc gctgacccag
     6301 gcggacccgc ccgtgcaggc cgtcaccccg acgacgctca cgcagatcgc ggcgccgccg
     6361 tcgggcgaca tccgctacca ggaccggcgg ctgctggtgt tctacttcga cgcctcctcg
     6421 atgccgctgc ccgaccaggt ccgcgcgtac tcgaacgcac tgaagtacat cgacacgcag
     6481 atgacgccat cggacctcgt agcgatcatg gcgttccaga acggcgccgt ctccgtgaag
     6541 accgatttca ccgacgaccg caacaagctg cgcgaggtga tccagatcct cacctacggc
     6601 gacgaccaga acaacgacgg gattcccgat catcccgaag ggacggcgtt cggccagaac
     6661 gatgccgagt tcaacatctt caacaccgac cggcagctcg cggcgctgca gacggcgttg
     6721 gcgatgctgc ggccgctgcc cgagctgaag acgctcatct acttcgcgag cggcctccgg
     6781 ctgaacggcg ccgacaacga ggcgcagctg cgcgccacgg tcaacgcggc gatccgggcc
     6841 aacgtcacgc tcaacccggt cgacgcgcgc gggctcgtgg ccacgccgcc gctcggcgac
     6901 gcgacgcgcc gatccccggg cggcgtcgcg atgttcagtg gaaagctcgc ggaagccatg
     6961 gtcaccaact tcgcgcgatc gcaggacacg ctgtacgcgc tcgcaaagga cacgggcggc
     7021 acggcgatgt tcgactacaa cgacctgtcg ctcggaattc agcgcgcggc gcaggccgtg
     7081 agcgactact acatcctcgg ctactacagc acccacgtcg agaacgacgg gaagttcagg
     7141 cgcgtgcgca tctcgctgag cggcggccgc acgggcgagg tcgcccaccg gcagggttac
     7201 ttcgccgaca agtcgttcgc ccgcttcaac gattccgaca aggagcggca gctcgaggac
     7261 gcgatgatgc tgggcgaccc ggtcaccgac atcacgattg cgatggaggt gaacttcttc
     7321 aagctcaata gcgcggagta tttcgtcccg gtggccgtca agatccccgg tagcgagctc
     7381 gagctggcgc ggcgccgggg cgccgcccgg acgctcatcg acttcatcgg cgaggtcaag
     7441 gacggctacg gctacacgat tcagaacatc cgcgacaggc tggacatcaa gctgagcgaa
     7501 gagaccgcca cgcagctggc gacacggccg atccagtacg agaccggctt cacgctgctg
     7561 ccgggcaagc acgtgatcaa gatcctggcg cgcgactcga ccacgggccg catcggcacc
     7621 taccaggcgt cgttcaccat tcccaacctg atgaaggaag cgcagcgcct gcccatcagc
     7681 tccgtggtgc tgtcgagcca gcgtgtgccg atgaccgacg cgctccacac ggtccagacc
     7741 ggcgcggcgc aactcaccca cccgctggtc ttcgacgggc agaagctctt ccccagcgtg
     7801 acccgcgtct tcagccgggg gcgcgagatg ttcgtctacc tgcaggccta cgagcaggcg
     7861 gccacgtcca cccagccgct cgtcgcgttc gtgtccttct tcagggacgg gcacaaggtg
     7921 ctggagaccg agccgctcgg catcaccgac ggcctcgacc cgaaatcgaa ggcggtgccg
     7981 atccggctca gcattccgct cgagggactc gctcccggcc gatacgactg ccaggtcacc
     8041 gtgctcgagc ccacggggcg cagagccgcg ttctggcagg cgccgatcgc agtggtaccc
     8101 tgacgagtag tccatttacc gtcgttcaca tttctcgctt ctcacttctc tgttcgtgtt
     8161 cacgttcgtg ttcgcgagtg ccgatacctc catcatgacc cttcttcgaa gacgctgtgc
     8221 ccggtttgtc gcggtcgcga tcgtcgtggc cgcaacggcc agcctcgccg cgcaggcgac
     8281 ggccccgtct ggcaagagtt ttctctggaa gatccaggcc ggctcgaatg tcgtctatct
     8341 ggccggctcc gttcatgcgc tcaacgccga cgcgtacccg ct
//
LOCUS       DBNGSH010000961         3688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4205538, whole genome shotgun sequence.
ACCESSION   DBNGSH010000961 DBNGSH010000000
VERSION     DBNGSH010000961.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4205538"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1059)
                     /locus_tag="AC16CY_22010"
     CDS             complement(7..1059)
                     /locus_tag="AC16CY_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356542.1"
                     /translation="MRRLSPAIVLLTVCTLVAMWPAPARAQAKGSADAAAARRTNPHW
                     KAPRTAWGHPDLEGIWTTDDMRGVPMSRPQQFGTRIYMTDDEFAARAKQRGNARDVDN
                     ARTGTFRNEEGSRDFSYTSMVIDPPDGRVPPITAAARARPRLQGSFGVGPWEKVQDFG
                     LYDRCITRGALGSFMPAVYGNGARIVQTPDSVVISYEMVHDTRVIPLDGRPPIGHGIH
                     LFMGDSRGHFEGDTLVVESRNFTDRTAVGGATHSEELRMVERFTRVDPEMIDYEIRID
                     DPATFTSPWTMRMTITSQPDYQIYEYGCHEGNIAMRNALSAERAYETAAAEAAAKGLP
                     PPERVFERVNGPDRGR"
     gene            1209..1556
                     /locus_tag="AC16CY_22015"
     CDS             1209..1556
                     /locus_tag="AC16CY_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3356543.1"
                     /translation="MQLSAIQTLADPCRFRIVETLLSGEQAVNDIVDRMDIHQSGVSR
                     HLRILEEAGFVRMRPDGQRRLYSLRPEPFRELEAWARQYRALWEGRLDAFATALERRQ
                     HARTRQRRRRRNQ"
     gene            1553..2044
                     /locus_tag="AC16CY_22020"
     CDS             1553..2044
                     /locus_tag="AC16CY_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3356544.1"
                     /translation="MRKRISLERVYHADVQDVWDLWTTKEGIESWWGPGGFAVTVRKL
                     DLRPGGELLYAMTAIDPPQVEFMKKAGMPLSHDARITFTEIVPRRRLAYVHLADFIPG
                     VDPYDVATVVELHPTERGVRMVLTFDAMHDEEWTRRATMGWESELGKLEALLGARIGG
                     GRS"
     gene            2041..2457
                     /locus_tag="AC16CY_22025"
     CDS             2041..2457
                     /locus_tag="AC16CY_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356545.1"
                     /translation="MTESEAPGHRGRSVGAVVAGFALTAGLSLGMDAVMHATGVFPPW
                     GEPMSDGLFAWATVYRIAFTVAGGYLTANLAPRRPMRHVIALGVVGLVAAAAGAAATW
                     NAGPAFGPRWYPLALVVTALPCVGAGGLLQGYLNRA"
     gene            complement(2681..>3688)
                     /locus_tag="AC16CY_22030"
     CDS             complement(2681..>3688)
                     /locus_tag="AC16CY_22030"
                     /inference="COORDINATES: protein motif:HMM:NF014871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3356546.1"
                     /translation="TDLSSCDMTAEIVRVARSLPPGLVDVIVGGHSHDLMGHQIEGVA
                     ISEAYSAGRAFSRVDVIVDRATRRVLERRSFAPRDLCARVDPGTANCAPDAAASARVP
                     AEYEGAPVTPDPAITKVIAPAIEAAATLKKSPVGVTLDTPVRIGDIGNSPLGNLFTDA
                     YLAAVPGADVAINNTDGGLRTDLPAGPLTYGAVFEVMPFDNRVVALRLTGAELRTVLE
                     TQLRAGAPLVGLAGLRARGTCRGGSIDVALLRPDGARVRDAEPLLVVTTDFLATGGDR
                     VFDPVKPSGGFAIVRDAGGARDIVVEMLRKRGGTLREGDLIDAANPRWVLPGPQPVTC
                     R"
BASE COUNT          569 a         1208 c         1271 g          640 t
ORIGIN      
        1 gggcgatcag cggccgcggt cggggccgtt gacgcgctcg aacacgcgct ccggcggcgg
       61 cagccccttg gcagcggctt ccgccgccgc agtctcgtaa gcgcgctcgg cgctcaacgc
      121 gttgcgcatc gcgatgttcc cctcgtggca cccgtactcg tagatctggt agtccggctg
      181 gctcgtgatc gtcatccgca tcgtccatgg gctggtgaag gtggcgggat cgtcgatgcg
      241 gatctcgtag tcgatcatct cgggatcgac gcgcgtgaag cgttcgacca tgcgcagctc
      301 ctcgctgtgc gtggcgccgc cgaccgccgt cctgtccgtg aagttccgcg actcgacgac
      361 gagcgtgtcc ccttcgaaat ggccgcgcga atcgcccatg aagagatgga tgccgtgacc
      421 gatgggcggc ctgccgtcga gcggaatcac ccgcgtgtcg tggaccatct cgtagctgat
      481 gacgaccgag tctggcgtct ggacgatgcg cgcgccgttg ccgtagacgg cgggcatgaa
      541 ggaaccgagc gcgccgcgcg tgatgcaccg gtcgtagagc ccgaagtcct ggaccttctc
      601 ccacggtccc acgccgaacg atccctggag acgaggccgc gcccgcgccg cggccgtgat
      661 gggcggcacg cggccgtcgg gcggatcgat caccatcgac gtgtagctga agtcgcggga
      721 gccttcttca ttgcggaatg tgccggtcct cgcgttgtcg acgtcgcgcg cgttgccgcg
      781 ctgctttgcg cgcgcggcga actcgtcatc cgtcatgtag atgcgcgtgc cgaactgctg
      841 cgggcgcgac atcggcacgc cgcgcatgtc gtcggtcgtc cagatgccct cgaggtccgg
      901 gtggccccac gccgtgcgcg gcgccttcca gtgcggattc gtccgccgcg cggccgccgc
      961 gtcggccgac cccttggcct gggcgcgcgc cggcgccggc cacatcgcga ccagggtgca
     1021 gacggtgaga agaacgatgg ccggggacag gcgccgcatc atggagacct ccaccgcgtc
     1081 ctattgtgca ctcaattcga gttttgagtt ttgagttttg agtctcgagg tttgaggttt
     1141 gagttccgag tgtcgagcaa cgctccgctt gacacagtat atatgcacta tgatacatat
     1201 acgcatcaat gcaattgagc gccatacaga cgcttgccga cccgtgccgc ttccggatcg
     1261 tcgaaacgct gctctccggg gagcaggccg tcaacgacat cgtggacagg atggacatcc
     1321 atcagtccgg cgtgtcgcgt cacctgcgca tcctggagga agccgggttc gtgcgcatgc
     1381 ggcccgacgg ccagcggcgc ctctattcgc ttcgcccgga gccgttcagg gagctcgagg
     1441 cgtgggccag gcagtaccgc gcgctctggg aagggcggct cgacgcgttt gcgacagcgc
     1501 tcgagcgacg ccagcacgcc aggactcgac agcgccggag acggaggaac caatgagaaa
     1561 gcgcatctcg ctcgaacggg tctatcacgc cgacgtccag gacgtctggg acctctggac
     1621 caccaaggag ggcatcgagt cgtggtgggg ccctggcggg ttcgccgtca ccgtgcggaa
     1681 gctcgatctc cgcccggggg gagagttgtt gtacgcgatg acggcgatcg atccgccgca
     1741 ggtggagttc atgaagaagg cgggcatgcc gttgtcgcac gacgcgcgca tcacgttcac
     1801 cgagatcgtt ccacgccgcc gcctggcgta cgtgcacctg gccgatttca tacctggtgt
     1861 tgatccctac gacgttgcca ccgtcgtgga acttcatccc accgagcggg gagtgcgaat
     1921 ggtcctgacc ttcgacgcga tgcacgacga agagtggacg cggcgcgcga ccatgggctg
     1981 ggagagcgag ctgggcaagc tcgaagccct gctcggcgcg cgcatcggcg ggggacgatc
     2041 gtgaccgaat cagaggcgcc gggtcatcgc gggagaagcg tcggcgcggt cgttgccgga
     2101 tttgcgctga ccgcagggct ctcgctgggc atggacgcag tcatgcacgc gaccggcgtc
     2161 ttccccccgt ggggcgagcc gatgtcggac ggactgttcg cgtgggccac cgtctaccgg
     2221 atcgccttca ccgtggcggg cggctatctg acggcgaatc tggcgccccg ccggccgatg
     2281 cgccacgtca tcgcgctcgg cgtcgtcggt ttggttgcgg cggcggcggg tgcggccgcc
     2341 acctggaacg cggggcccgc ctttggtcca aggtggtatc cgctcgcact cgtcgtgacg
     2401 gcgttgccct gcgtgggggc gggcggcctc cttcagggtt acttgaatcg agcgtgagct
     2461 cagtcgttca cccttctcgc ttctcgcttc tctgttcgtg ttcagcttca tgttcgtgtt
     2521 ctgggttcct ggttcacacg gtccggctaa agccggacac tacgatcgat tggtgctcgg
     2581 cgtacgaagt gtccggcttc agccggactc gcgaacatga acacgaaccg agaagtgaga
     2641 agcgagaagt gtgaacgcga gatctcgtca agcttcgacg ttaccggcag gtgacgggct
     2701 gaggtcccgg cagcacccat cgcgggttcg ccgcatcgat caggtcgccc tcgcgcagcg
     2761 tcccgccgcg ctttcgaagc atctcgacga cgatgtccct tgcgccgccg gcgtcgcgca
     2821 cgatcgcgaa gcctcctgac ggcttcaccg gatcgaagac gcggtcgccg ccggtggcca
     2881 ggaagtccgt tgtcaccacg agcaacggct ccgcgtctct gactctcgcg ccgtccggcc
     2941 gcagcagcgc gacgtcgatc gacccgcccc ggcatgtacc gcgcgcgcgc aggccggcca
     3001 ggcccacgag gggcgcgccc gcgcgaagct gcgtctcgag gacggtgcga agctcggcgc
     3061 cggtcagccg gagtgcgacc acgcggttgt cgaacggcat cacctcgaag accgcgccgt
     3121 aggtgagcgg accagcgggc aggtccgtgc ggagaccgcc gtccgtgttg ttgatcgcga
     3181 cgtcggcgcc gggaaccgct gcgaggtacg catccgtaaa gagattcccg agcggcgaat
     3241 tgccgatgtc gccgattcgt accggcgtgt ccagcgtgac gccaacgggc gacttcttca
     3301 gagttgccgc tgcttcgatt gccggcgcga tcactttggt aattgcggga tcgggcgtga
     3361 cgggagcccc ctcatactcg gccggcacgc gcgcgctggc cgcggcatcc ggggcgcagt
     3421 tggccgttcc cgggtccact cgcgcgcaga gatcgcgagg cgcgaagctg cgccgctcga
     3481 gcacccgcct cgtcgcgcga tcgacgatca catcgacgcg gctgaaggcg cggcctgctg
     3541 aatacgcctc ggagatggcc acgccctcga tctggtgacc catcaggtcg tgcgagtggc
     3601 cccccacgat gacatcgacg aggcccggcg gcagcgaacg tgcgacgcgc acgatctctg
     3661 cggtcatgtc gcaggacgag aggtcggt
//
LOCUS       DBNGSH010000962         6409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1488160, whole genome shotgun sequence.
ACCESSION   DBNGSH010000962 DBNGSH010000000
VERSION     DBNGSH010000962.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1488160"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="AC16CY_22035"
     CDS             <1..1877
                     /locus_tag="AC16CY_22035"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3356547.1"
                     /translation="VLMLATVPIFQRLGSEFMPPLDEGTLLVMPTTFPGISIEEARRA
                     LQRQNRTIKSFAEVASVHGKVGRAETATDPAQLDMIETVITLRPRDEWPTRHTHRWFS
                     DDAPEWLKPMLRTVWPEERARTLAELSRDLDAALRMPGYQMAIAPPIRTRIDMLTTGV
                     RTPVGIKVFGSDLGEIERVSIALEGMLREVRGTRSTFAERQTGREYVDIVPNRDAIAR
                     YGLTVRDVQDVVEAAVGGMPVSTAIAGRARFSINLRYAADRRSDPQALRRILVPVQST
                     AGVSDFGGGNVGGGVSSPTAGMVTSATAAAGGGMGAMGGRSASTGMQPANAMGASQPV
                     LPMGGSQGATDLLEQWRQPGAAVPLGELADVRVVTGPPMIKDENGVLVGYVFADIDQT
                     QRDLGGWVDDAKALVAARLQLPPGYRLQWTGQYEFLAQMEARLRYIIPLTLVLVVALL
                     YMSMRGWPQTLLVLSSLPFAVAGSIWLLAFMHYNLSTAVWVGLIAVAGVAAETGIVMV
                     VYLDEAFDRYMREGRIQGPEDVDAAVVEGASARVRPLLMTVATTVLGLLPLLWEAGVG
                     ADVSARTAAPVVGGLWSCMFLTLLVLPAAYAMWRRHQVRATLPLSLAHDAVTPSEGAA
                     "
     gene            2000..2665
                     /locus_tag="AC16CY_22040"
     CDS             2000..2665
                     /locus_tag="AC16CY_22040"
                     /inference="COORDINATES: protein motif:HMM:NF024911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HIR3356548.1"
                     /translation="MVPCECATVHDRRRVVLTGGPGAGKTAVLELIRQSFCEHVKVLA
                     EAAGVVFGGGFPREDDPGCTRAAQRAIFYVQRELENAGDSHNPAIVLCDRGTLDGLAY
                     WPSPVDEFWTTLGTTRSEQLARYHAVIHLRTPGLDHGYNNQNPLRTESAAAAAEIDQR
                     ILQAWEAHPRRFVIESTAAFLDKAAEALQIVRGEMPACCRRHVIPALSEHAKTAGVTQ
                     VAR"
     gene            3298..3993
                     /locus_tag="AC16CY_22045"
     CDS             3298..3993
                     /locus_tag="AC16CY_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3356549.1"
                     /translation="MTEHRRRSGRRMPFRNAASAAKDGHWSATIRALLLLVVLISAVG
                     GVIAYSIVSRGLSAHDEPSRVETMLARAMRRWATPGSLRNRTNPVEPTEAVLEQALSH
                     YADHCASCHANNGSGDTAIGRALYPRVPDMRASATQSLTDGELFSIIEHGIRLTGMPG
                     WGNGTPEGERDSWGLVHFVRRLQKLTDEDIERMDALNPKSPAELKEEEEARRFLRGDS
                     AKPSDGSSKDSQH"
     gene            4001..5803
                     /locus_tag="AC16CY_22050"
     CDS             4001..5803
                     /locus_tag="AC16CY_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HIR3356550.1"
                     /translation="MRSQPYSSRRRSSEGTSRRTFVKGLAVGGTVAALGLWRASAFAQ
                     GNTPSRWGDVSGTEFDLRIGETPMNITGNARSAVTVNGSIPAPTLHWKEGDTVTLRVA
                     NTLSEDASIHWHGILLPANMDGVPGLSFHGIRPGETYVYRFTVRQGGTYWYHSHSGFQ
                     EQRGLYGPLVIHPREPEPFGYDREHVVMLTDWTDESAERVFAKLKKRSDYYNFHQRTV
                     GDFFRDVSEDGLKSALADRKMWGEMRMNPTDLADVSGYTYTYLLNGVTPAGNWTGLFK
                     PGERVRLRFINGSAMSYFDVRIPGLQMTVVAADGQYVHPVTVDEFRIATAETYDVIIE
                     PKAQDAFTIFAQSMDRTGFAGGTLAVREGLRAELPTLDPRPRLTMADMGHGGKGAGHA
                     ASPTAAADPHAGHQTPPKTPADPHAGHDMSAMSAVQQHPPSEKGNPLVDMQTMAPASR
                     LDDPGIGLRDNGRRVLRYADLRNTFADPDGREPGRTIELHLTGHMQRFAWSFDGIRFS
                     DAEPLRLTYGERLRIVLVNDTMMTHPIHLHGMWSDVEDDEGRFHVRKHTVDMPPGTKR
                     SYRVTADALGRWAYHCHLLYHMESGMFREVRVDE"
     gene            5800..>6409
                     /locus_tag="AC16CY_22055"
     CDS             5800..>6409
                     /locus_tag="AC16CY_22055"
                     /inference="COORDINATES: protein motif:HMM:NF017119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HIR3356551.1"
                     /translation="MIRRVFYAILAVGSLTCGVTRPASAQDAHTGHQQEKPARQPTPA
                     SDQHQHQASTDLPSFIPPLTDEDRKAAFPDVQGHAVHENAVNYLVLFDQLEWQAGEGA
                     NGISIDTRGWVGRDRDRLWFRAEGDGDDGGVGEAQTHVLYGRRFSRWWDVVAGIRQDF
                     RPGSPQTWAAFGVQGLAPYWFEIEATAYVGASGRTHARFEVEY"
BASE COUNT         1140 a         2059 c         2112 g         1098 t
ORIGIN      
        1 ccgtgctgat gctcgccacc gtcccgatct ttcagcgact cggttcagag ttcatgccgc
       61 cgctcgacga aggcacgctg ctcgtcatgc cgaccacctt tcctggcatc tcgatcgagg
      121 aagcgcggcg cgccctgcag cggcagaacc ggacgatcaa gagcttcgcg gaggtggcgt
      181 ccgtccacgg caaggtgggc cgcgcggaaa ccgccaccga tcccgcgcag ctcgacatga
      241 tcgaaacggt tatcactctt cgtccacggg acgagtggcc gacgagacac acacaccgct
      301 ggttcagcga cgacgccccc gagtggttga agcccatgct ccgtacggta tggcccgagg
      361 agcgtgcccg taccctcgcc gagctgtcgc gcgatctgga cgccgcgctc cgcatgcccg
      421 gctatcagat ggcgattgcg ccgccgatcc gcacgcggat cgacatgctg acgacgggcg
      481 ttcgcacacc cgtcggcatc aaggtgtttg ggagcgacct cggtgagatc gaacgcgtca
      541 gcatcgccct tgaaggcatg ctccgcgagg tgcgcggcac gcggagcacc tttgcggagc
      601 gacagactgg ccgcgagtac gtcgacatcg tgcccaatcg tgacgccatc gcacggtatg
      661 ggctcaccgt ccgtgacgtg caggacgtgg tggaagccgc tgtcggcggg atgccggtgt
      721 caacggcgat cgccgggcgc gcgcggttct cgatcaatct gcggtatgcc gcggatcgcc
      781 gatccgatcc gcaggcgctg cgccgcatcc tcgttccagt ccagtcgacc gcgggcgtct
      841 ccgacttcgg cggcggcaat gtgggcggcg gtgtctcgtc gccaacggca ggaatggtga
      901 cgtccgccac tgcagcagcc ggtggcggaa tgggcgcgat gggcgggcgg tctgcctcaa
      961 ccggaatgca gcccgcaaac gcgatgggcg cgtcacaacc cgtacttcct atgggcggat
     1021 cacaaggtgc aacggatctg ctcgaacagt ggcgccagcc aggtgccgcc gttccactcg
     1081 gcgaactggc cgatgtgaga gtcgtcactg gcccgccaat gatcaaggac gagaacggcg
     1141 tgctcgtcgg atacgtgttc gcagacatcg accagacaca gcgcgacctc ggtggctggg
     1201 tcgacgacgc caaggctctt gtcgcggctc gattgcagct ccctcccggg taccgcctgc
     1261 agtggaccgg gcaatacgaa ttcctcgccc agatggaagc gcggctccgc tacatcatcc
     1321 cgctgacgct cgtgctcgtg gtcgcactgc tttacatgtc gatgcggggc tggccacaga
     1381 cgctgctcgt cctctcgagc ctgccctttg ccgtcgctgg cagcatctgg ctgctggcct
     1441 tcatgcacta caacctctcg accgccgtgt gggttggact gatcgccgtg gccggcgtcg
     1501 ccgccgagac cgggatcgtc atggtcgtct atctggacga agcgttcgat cgctacatgc
     1561 gcgagggccg cattcagggg cccgaagacg tcgatgccgc ggtcgtcgaa ggagccagcg
     1621 ctcgcgtccg gccgctgctg atgacggtgg caacgacggt gctcggcctg ttgccgctgc
     1681 tctgggaggc aggcgttgga gcggacgtct cggcccgcac agctgccccc gtcgtcggag
     1741 gcctctggtc ctgcatgttt ctgacgctgc tcgtgctccc ggccgcctac gcgatgtggc
     1801 gtcgtcatca ggtgcgcgcc acgctcccgc tgtccctggc gcacgatgcg gtcactccgt
     1861 cggaaggcgc cgcatgactc gattgacgct cgtcgagttc gctcccgcgt cgtcttccaa
     1921 cgagctaccc gcaagggagg gcggcgagcg aggaggacac cgtgacgacc agcattgact
     1981 acgatcccga ggaggaacca tggtgccgtg cgagtgcgcc accgtgcatg accggcgacg
     2041 ggtcgtgctg acaggcggac caggtgcagg gaagaccgca gttctcgagc tgattcgcca
     2101 gtcgttctgc gagcacgtca aggtgctggc tgaggccgcc ggtgtcgtct tcggcggtgg
     2161 cttcccgcgc gaggacgatc ccggatgcac gcgcgcggcg caacgggcga tcttctacgt
     2221 gcaacgcgag ctcgagaacg ccggcgacag ccataatccg gcgatcgtgc tgtgcgatcg
     2281 cggaacgctc gacggtctcg cgtattggcc gagccccgtc gatgagttct ggacgacgct
     2341 tggtacgacc cgttcggagc aactggcccg gtaccacgca gtgatccatc tacgcacgcc
     2401 gggattggac cacgggtaca acaaccagaa tccactcaga acggaatctg cggctgctgc
     2461 ggccgaaatc gaccagcgga ttctgcaagc ctgggaggcg caccctcgac gcttcgtgat
     2521 cgagtccacg gcggcgtttc ttgacaaggc ggcagaggcg ctccagatcg tacgaggtga
     2581 gatgcccgcg tgctgcagac gtcatgtcat ccccgcgctg agcgaacacg cgaagaccgc
     2641 cggcgttaca caggttgcga ggtaggagcc caacgacggt cgaggccggc ggctgtcatc
     2701 gattccgaag gtctgcgttg aggggctgaa ggccactgaa tgaaacgccg cgcatggctg
     2761 tgccggcgtc ctgaagtccc gcggtatact cgatccgtgt gttggcgaca caggattgcc
     2821 gttgtccttc tgaccgttct ggccggcgtg ccggtttcgg gaaccgtctg tgtgatggtg
     2881 tgcgaatcgg catccggcgc agcggccggc caccacggtt caggacagac gtgtgaggag
     2941 ccctcgcttc catcgactgg accccacctg agcggcccgt cgcagcacga ctgccgcgcg
     3001 cacgccggcg tcattccgca ggcagcgacg acagccgcgg aacgagctga caccatcgcg
     3061 aaatctcccc tacaggtgct cggcgccgtt cccgtccggc ccgtgacgct gcgcgactct
     3121 gggaccttcg acgacacttc tccgccgggt acgactccac tcacaacgac gccgctcgtt
     3181 ttgcgcgtct gatcccgatc gcataggcgc atagcgccaa gtaatcgcgt ggcacggtgt
     3241 tgacaccgtt gcattgccgt gtcatcgcac gtcgcgtgtg tgggggaagg gatcttcatg
     3301 acggagcatc gacgtcgttc cggccgacgg atgccgttca gaaatgctgc cagtgcagcg
     3361 aaagacggcc actggagtgc aaccattcgt gcacttctgc tgctcgtcgt tctgatttcc
     3421 gcggtcggag gcgtcatcgc ctactcgatc gtgagccgcg gcctgagcgc tcatgacgaa
     3481 ccgagtcgcg tcgagacgat gctcgcgcgg gccatgcgcc gctgggcgac gccggggtcg
     3541 ttgcgcaacc ggaccaatcc cgtcgagccc acagaagcgg tcctcgaaca ggcgctgtcg
     3601 cactacgccg accattgcgc ctcctgtcac gcgaacaacg gcagcggcga cacggcaatc
     3661 gggcgcgctc tctatccgcg agtgcccgac atgcgcgcca gtgcgacgca gtcgctgacc
     3721 gacggcgagc tgttctcgat catcgagcac gggatccggc tcaccggtat gcccgggtgg
     3781 ggaaacggca cgcccgaggg cgagcgcgac agctggggcc tcgtgcactt cgtacgaagg
     3841 ctccagaagc tgactgacga ggatatcgag cgcatggacg cgctcaaccc gaagtcgccg
     3901 gccgaactga aggaagagga agaggctcgc cgcttcctgc ggggggactc cgccaagccg
     3961 tcagacgggt cctcgaaaga ttcgcagcac tgaggaatca atgaggtcgc agccctactc
     4021 atctcgccgc cgatccagcg aaggcacgtc gcgacgaaca ttcgtcaagg gcctcgcagt
     4081 tggagggacc gtcgcggccc ttggcctgtg gcgcgcgtcg gcattcgcgc agggcaacac
     4141 cccgtcgcgg tggggtgatg tgtcgggcac cgagttcgat ctgcgaatcg gcgagacccc
     4201 aatgaatatc acggggaacg cgaggagcgc ggtcaccgtg aacggctcga ttcctgcgcc
     4261 cacgttgcac tggaaggaag gagacacggt gacactccgt gtcgccaaca ccctctctga
     4321 agacgcctcg attcactggc acggcattct gctgcccgcg aacatggacg gcgtgcccgg
     4381 tctcagcttt cacggcatac ggccgggcga aacctacgtc taccgattca cagtgcgtca
     4441 gggagggacg tactggtacc acagccactc cgggttccag gagcaacgag gtctgtacgg
     4501 accgctggtc atccacccgc gcgagccgga accattcggg tacgaccgcg aacatgtcgt
     4561 gatgctcacg gactggacgg atgagagcgc cgagcgcgta ttcgcgaagc tgaagaagcg
     4621 ttcggactac tacaactttc atcagaggac tgtcggagac ttcttccggg acgtcagcga
     4681 ggatggcctg aagagcgccc tcgccgatcg aaagatgtgg ggcgagatgc gcatgaatcc
     4741 aacggacctc gccgatgtgt ccggatatac gtacacgtac cttctgaatg gtgtgacccc
     4801 ggcaggcaat tggacggggc tcttcaagcc aggagagcgt gtgcgcctgc gtttcatcaa
     4861 tgggtccgcg atgtcgtact tcgacgtgcg gattccagga ttgcagatga cggtggtggc
     4921 tgcggatggt cagtacgtgc atcccgtgac ggttgatgaa ttcagaattg ccacggcgga
     4981 gacctacgac gtcatcatcg agcccaaggc tcaggacgcg ttcacgatct tcgcgcaatc
     5041 catggatcgc acggggttcg ccggtggcac gcttgcggtg cgcgagggcc ttcgggccga
     5101 gcttcccacg ctcgacccga ggccgcggct cacgatggcg gacatgggcc acggcgggaa
     5161 gggggccggc cacgccgctt cgcccacggc tgccgctgat ccgcacgcag ggcaccagac
     5221 gccgccgaag acgccggctg atccgcatgc gggccacgac atgtccgcga tgagcgccgt
     5281 gcaacagcac cctccaagcg agaagggcaa tccgctcgtc gacatgcaga cgatggcacc
     5341 ggcatcgcgg ctcgacgatc ccgggatcgg acttcgcgac aacgggcgcc gggtactccg
     5401 ctacgcggac ctgaggaaca ccttcgcaga tcccgacgga cgtgagcccg gccggacgat
     5461 tgaattgcat ctgaccgggc acatgcagcg cttcgcgtgg tcgttcgacg gcatcaggtt
     5521 ctcggatgct gagcccctcc ggcttaccta cggcgaacgg ctccggatcg tgctcgtcaa
     5581 cgacaccatg atgacccacc cgattcactt gcatgggatg tggagcgatg tcgaggacga
     5641 cgagggcagg ttccacgtgc gcaagcacac cgtcgacatg ccgccgggca cgaagcgatc
     5701 gtatcgggtg acggccgatg cgctgggccg gtgggcgtat cactgccacc tcctttacca
     5761 catggaatcc ggcatgttcc gggaagtgcg ggtggacgaa tgattcgtcg cgtgttctac
     5821 gccattctcg cggtcggatc gctcacgtgt ggtgtgactc ggcctgcctc tgctcaggat
     5881 gcccacactg ggcatcaaca ggagaagccc gcgaggcaac cgacaccggc gtcagaccag
     5941 caccagcatc aggcttcgac cgatctgccg tcgttcatcc caccgctgac cgacgaagac
     6001 cggaaggccg cgttcccgga tgtccagggg catgcggtgc atgagaacgc agtgaactac
     6061 ctggtgctct tcgatcagct cgagtggcaa gccggcgagg gcgcaaacgg catcagcatc
     6121 gatacacgcg gatgggtggg gcgggatcgc gaccgcctgt ggttccgcgc cgaaggcgat
     6181 ggcgacgacg gcggtgttgg cgaagcgcag acacacgtgc tgtacggacg ccggttctcg
     6241 cgttggtggg atgtggttgc cggcatccgt caggatttcc gcccgggctc gccgcaaacc
     6301 tgggcggcct ttggcgttca aggcctcgcc ccgtattggt tcgaaatcga ggcgaccgcg
     6361 tacgtcggtg cctcaggcag aacccatgct cgcttcgagg tcgagtatg
//
LOCUS       DBNGSH010000963         2777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_469576, whole genome shotgun sequence.
ACCESSION   DBNGSH010000963 DBNGSH010000000
VERSION     DBNGSH010000963.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_469576"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1556)
                     /locus_tag="AC16CY_22060"
     CDS             complement(18..1556)
                     /locus_tag="AC16CY_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR3356552.1"
                     /translation="MPRDRYDIIIIGTGAGGGTVAQALAGSGARMLIIERGTFVPQEE
                     ENWDPAAVWKDLRYRTREEWLDDQGRAFLPYTHYCVGGNTKFWGSVLYRLRREDFQAV
                     EHVDGVSPAWPIQYETLEPYYERAERLYQVHGGSGDDPTEPPRRAPYPFGPIAHEPAM
                     EEIVERLRGQGLHPSPLPLGLIRPGEPGGCVRCNTCNSFPCRVNAKSDADVLCVRPAT
                     TFPNVTLWTQTIARRLLTDPSGKRVEAVEVERAGETIQAEASLFVVSCGAVNSAALLL
                     RSSSPAHPAGLANSSGLVGRRYMAHLATMMQGFHPFRRNSIVFQKTVAINDYYLRGVD
                     APYPLGQIQSQGRTHGVMAQTVAPQIPLWAYDAWVARGVDWLAMTEDLPRDENRVTVT
                     SDGRIKLEYRPNNVTAHRQLVKELTRILKRLGYWIVMKHSHENRNTTHQCGTLCFGDD
                     PRTSVLDPYCRTHDVRNLFVVDASFFPSSAAVNPGLTIAAQALRVAEHIELAGRREPV
                     AGSL"
     gene            complement(1568..2578)
                     /locus_tag="AC16CY_22065"
     CDS             complement(1568..2578)
                     /locus_tag="AC16CY_22065"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3356553.1"
                     /translation="MPLTPAPVHIAFLGCGFITGVHSRHLRALRSDVVCSYASRDGEK
                     AEAFRRRYGGLKSYAGYAQAMADTKVDAVVIAVPPRFHLDLTLQALSADKHVLVEKPA
                     YPRMADYQRVLEAKSRAQRIVLVGENDHYKPLAVCLRALLREGAIGEMLFAHFTTLVK
                     KFKQAGDWRNDEAMAGGDAFFEEGIHWLHLAASLGPRIVRIEGFRPTPSPGGADARAK
                     SMMVAFAYDNGAVGSLYYSREVPSLLRGLRLSALSGRDGIITFESNGLFVFVRGRGLP
                     RLLLPGFRDIRGYRAMYRDFVSAMRQQRPPQMSLERAMDDQRLMDRIYESLAHGALAA
                     SR"
     gene            complement(2595..>2777)
                     /locus_tag="AC16CY_22070"
     CDS             complement(2595..>2777)
                     /locus_tag="AC16CY_22070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356554.1"
                     /translation="ATMIANAKTFLTTVQARAGELKKQGRSLDETVKLVQDELQSRYD
                     RNRMAGAIRAAYNEAP"
BASE COUNT          455 a          963 c          888 g          471 t
ORIGIN      
        1 gagcgcgctc tcatacgcta caggctacca gctaccggct cccgcctgcc ggccagttca
       61 atgtgttccg ccacgcgcaa tgcctgcgcc gcgatcgtca gccccgggtt cacggccgcg
      121 gacgagggaa agaacgaggc gtcaacgacg aacaggttcc gtacgtcgtg cgtccggcag
      181 tacgggtcga gcaccgatgt acgcggatcg tcaccgaaac agagcgtacc gcactggtgc
      241 gtcgtgttcc ggttctcgtg cgagtgtttc atcacgatcc aatacccgag acgcttgagg
      301 atcctcgtca actccttcac gagttgcctg tgcgccgtga cattgttggg tcggtactcg
      361 agcttgatgc ggccgtccga cgtcaccgtc acgcggtttt cgtcccgcgg gagatcctcg
      421 gtcatggcaa gccagtccac gcccctggcg acccacgcat cgtaggccca cagcggaatc
      481 tgcggcgcga ccgtctgcgc catgaccccg tgcgtacggc cctgcgactg gatctgcccg
      541 agcggatacg gcgcgtccac gccccgcaga tagtaatcgt tgatggcgac cgtcttctga
      601 aacacgatcg agttgcggcg gaacggatgg aaaccctgca tcatggtggc cagatgcgcc
      661 atgtagcgcc gccccaccag acccgaggag ttcgcgagcc cggccgggtg cgccgggctg
      721 gatgaccgca gcaacagcgc cgccgagttc acggcgccgc acgacaccac gaacagcgag
      781 gcctccgctt gaatggtttc gccggcccgt tccacctcga ccgcttcgac cctcttccct
      841 gacgggtcgg tcagcagccg gcgcgcgatg gtctgggtcc agagtgtaac gttgggaaac
      901 gtcgtcgccg gtcggacgca cagcacgtcc gcgtcgctct tggcgttcac ccggcaggga
      961 aacgagttgc acgtgttgca gcgcacgcag ccgccgggct caccgggcct gatgagacca
     1021 agcggcagcg gcgacggatg cagcccctgt ccgcgcaggc gctcgacgat ctcctccatc
     1081 gccggctcgt gcgcgatcgg gccaaacggg tagggcgcgc gcctgggcgg ctcggtcggg
     1141 tcatcgccgg atccgccgtg cacctgatac agtcgctcgg cccgctcgta gtacggctcc
     1201 agcgtctcgt actgaatagg ccaggccggc gacacgccat cgacgtgctc gaccgcctgg
     1261 aagtcctcgc gccgcagccg atagagcacg cttccccaga acttcgtgtt tcccccgacg
     1321 cagtagtgcg tgtacggcag gaacgcacgg ccctggtcgt cgagccattc ttctctcgtc
     1381 cggtagcgaa ggtctttcca cacggcggcc gggtcccagt tttcctcttc ctgcgggacg
     1441 aacgtgccgc gctcgatgat gagcatccgc gcgccgctgc cggcgagcgc ctgcgcgacc
     1501 gtcccgccgc cggcgccggt gccgatgatg atgatgtcgt agcgatccct gggcatcgtg
     1561 gagcggccta tcgtgaggca gccagcgctc cgtgtgcgag gctctcgtag atccgatcca
     1621 tgagtcgctg gtcgtccatc gcgcgttcga ggctcatctg cggcggccgc tgctggcgca
     1681 tggcgctcac gaagtcacga tacatcgccc gatagccgcg gatgtcgcga aagccgggca
     1741 gcaggagccg cgggagccca cggccccgca cgaaaacgaa cagcccgttg gattcgaacg
     1801 tgatgatgcc gtcgcggccc gacaacgccg agagccgaag ccctcgcagc agcgacggaa
     1861 cctcgcgaga gtagtacaac gagcccacgg cgccgttgtc gtaagcgaaa gccaccatca
     1921 tgctcttggc gcgcgcgtcg gcgccgcccg gagaaggcgt ggggcggaag ccctcgatgc
     1981 gaacgatgcg cggcccgagg ctcgccgcca ggtgcagcca gtgaatgccc tcttcgaaga
     2041 aggcgtcgcc accggccatc gcttcgtcgt ttcgccagtc gcctgcctgc ttgaacttct
     2101 tcaccagcgt cgtgaaatga gcgaacagca tctcgccaat cgcgccttcc cgcagcagcg
     2161 ctcgcagaca gacggccagc ggcttgtagt gatcgttttc gcccaccagc acgattcgct
     2221 gagcccgcga cttggcctcg aggaccctct ggtagtcggc catccgcgga tacgccggct
     2281 tctccaccag cacgtgcttg tccgccgaga gcgcctggag cgtcaagtcc aggtgaaatc
     2341 gcggcgggac ggcgatgacg acggcgtcca ccttcgtgtc cgccatcgcc tgtgcgtagc
     2401 ccgcgtagct tttcaggccg ccatagcgcc ggcggaacgc ctccgccttc tccccgtcgc
     2461 ggctggcgta gctgcacacg acgtcggaac gaagggcgcg aagatggcgg ctgtggacac
     2521 cggtgatgaa gccgcagccg aggaatgcga tgtgaaccgg tgccggtgtc agcggcatcc
     2581 gacgcgcaga ccggtcatgg cgcctcgttg taggccgccc ggattgcgcc cgccatccgg
     2641 ttgcgatcgt agcggctctg cagctcgtcc tgcaccagct tcaccgtctc gtcgaggctc
     2701 cgcccctgct tcttgagctc acccgcgcgc gcctgcacgg tggtcaggaa cgtcttcgcg
     2761 ttcgcgatca tcgtcgc
//
LOCUS       DBNGSH010000964         2446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2507718, whole genome shotgun sequence.
ACCESSION   DBNGSH010000964 DBNGSH010000000
VERSION     DBNGSH010000964.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2507718"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="AC16CY_22075"
     CDS             <1..309
                     /locus_tag="AC16CY_22075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356555.1"
                     /translation="ADDFVVSAEHRSGSRYHGQAAIFWDLGTVSGTIRVPPGWSVLDV
                     RVDQATEPRYVGQVSLGTFSRQGETLKFCGSEPGAQVRTQSFEAPPAGVRCLTLVRVG
                     "
     gene            complement(387..2267)
                     /locus_tag="AC16CY_22080"
     CDS             complement(387..2267)
                     /locus_tag="AC16CY_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HIR3356556.1"
                     /translation="MRTRMWASVVVVLACLLSALLVAQVDLGALHGTVVDATGLPIPG
                     ATVTITGPEKRTTVTDSRGEFSFVKLRSGDYEVVTGLTGFATSRIKASVGPGRIERVA
                     ITLTVGALTESVSVFAESRVLDANASAHRLVGGVAGGVVSGAIDGLPGRYNRAFNTEA
                     YDHIDENRFRRVEVDPLSTFSIDVDTASYANVRRFLTTGTRPPAGAVRTEELINYFRF
                     DYPQPSRDEPFSITTELVSSPWNPRHKLALIGLQGRELTGREPSPRNLVFLIDVSGSM
                     MPQDKLPLVRTAMRMLADVLTERDRVAIVVYAGASGLVLPSTSGDQKERIHSALERLE
                     AGGSTNGAAGIRLAYQTARDNFIPGGVNRVVLATDGDFNVGVTNQSELVTLIEQERAS
                     GVFLSVLGVGTGNLKDSTMEKLADKGNGNYAYLDTLHEARKVLVKEAGGTLETIAKDV
                     KIQVEFNPREVASYRLIGYENRLLKNEDFNDDRKDAGEIGAGHSVTALYEIVPAGMDT
                     PAPGVDPLKYQQEARETRAARSGELLTVKVRYKAPDGDTSRLLTKVLMNEASEMTANA
                     GFASAVAEFGMVLRGSPDIGTASFQSAAVRARRFKGTDAEGYRAEFIQLAERAARVVD
                     RR"
BASE COUNT          402 a          806 c          812 g          426 t
ORIGIN      
        1 gccgacgact tcgtcgtatc ggccgagcac aggtccggca gccgctatca cggacaggcg
       61 gcgatcttct gggacctcgg caccgtgtcc gggaccatcc gcgtgccgcc tggctggagc
      121 gtgctcgacg tgcgggtgga ccaggcaacc gaaccacgtt acgtcggaca ggtgtcgctc
      181 ggaaccttca gccggcaggg cgagacgctg aagttctgcg gctcggagcc gggcgcgcag
      241 gtccgtacgc agtcgttcga ggcgccgccg gcgggcgtgc ggtgcctgac gctggtccgg
      301 gtcgggtagc ccagaaaggg ggtcgggagt catttttcga gcgcgggtga acaacgcatc
      361 ctcgaaaatg actcccgacc ccattttcag cgacggtcca ccacgcgtgc agcgcgttcg
      421 gccagctgaa tgaactccgc gcgatagccc tccgcgtccg ttcccttgaa gcgcctcgcg
      481 cgtacagccg cggactggaa gctggcggtc ccgatatccg gagacccgcg aaggaccatg
      541 ccgaactcgg ccacggcgga ggcaaacccg gcgttggcgg tcatctccga cgcctcgttc
      601 atcaacacct tcgtgagcag ccggctcgtg tcgccgtcag gggccttgta tcgcaccttc
      661 acggtcaaca gctcgccaga cctcgcagcg cgcgtctctc gcgcctcctg ctgatacttg
      721 agcggatcga cgcccggcgc gggcgtgtcc atcccggcgg ggacgatctc gtagagcgcc
      781 gtcaccgaat ggccggcgcc gatctcgccg gcatccttgc ggtcgtcgtt gaagtcctcg
      841 tttttcagca gccggttctc gtacccgatc aaccggtacg acgccacttc gcgggggttg
      901 aactccacct ggatcttcac gtccttcgcg atcgtctcga gcgtgccgcc ggcttccttg
      961 acgagcactt ttcgcgcctc gtgcagcgtg tccaggtagg cgtagttgcc gttccccttg
     1021 tccgccagct tctccatcgt cgagtccttc aggttgccgg tgccgacgcc gaggaccgac
     1081 aggaagacgc cgctcgcgcg ctcctgttcg atcagtgtga cgagctcgct ctgattcgtg
     1141 acgccgacgt tgaagtcgcc gtcggtggcc agcacgacgc ggttcacgcc gcccggaatg
     1201 aagttgtcgc gcgcggtctg gtaggcgagc cgtatgccgg ccgcgccgtt ggtagacccc
     1261 ccggcctcga ggcgctcgag cgccgagtgg atgcgctctt tctgatcgcc ggacgttgac
     1321 ggaagcacga gaccgctggc gcccgcgtag acgacgatgg ccacgcggtc tcgctcggtg
     1381 aggacgtcgg cgagcatccg catcgccgtg cgcacgagcg gcagcttgtc ctgcggcatc
     1441 atcgagcccg agacgtcgat caggaacacc aggttgcgcg gcgacggctc cctgccggtc
     1501 agctcccgac cctgcagccc gatcagcgcc agcttgtgcc gtggattcca cggagacgac
     1561 accagctcgg tggtgatcga gaagggctcg tcacgggagg gctgcgggta gtcgaagcgg
     1621 aagtagttga tcagttcctc ggtgcgaacg gccccggcgg gcggccgggt gcctgtcgtg
     1681 aggaaccgac gcacgttggc gtacgaagcc gtatcgacgt caatcgagaa cgtcgagagc
     1741 ggatcgacct ccacacggcg gaagcggttc tcgtcgatgt ggtcgtacgc ttctgtgttg
     1801 aacgcacggt tatatcggcc gggcaggccg tcaatcgcgc cgctgacaac tccccccgcc
     1861 acgccgccaa ccaggcggtg ggcagaggcg ttcgcatcca gtacccgcga ttcggcaaac
     1921 acggagacgc tttcggtcaa ggcgccgacg gtcaacgtga ttgcgacgcg ctctatccgg
     1981 cctggcccca cggacgcctt gattcgcgac gtcgcaaacc cggtcaggcc ggtcacgact
     2041 tcgtagtcgc ccgagcgcag cttgacgaac gagaactccc cgcggctgtc ggtgacggtg
     2101 gtgcgctttt cgggtccggt gatggtgacc gtggcacccg gaatcgggag tccggtggca
     2161 tcgaccacag tgccatggag cgcaccgagg tcgacttgag cgacgaggag cgcggacaga
     2221 agacacgcga gaacgacgac aacagaagcc cacatgcgcg tacgcatggc ttcacctccc
     2281 aggcaggcgc gttatcgtcg atccggatcg gcgagtgaga ggcacgcccg caaagctcgt
     2341 gcctcgggca acccttatgc tggcttagac accgctgagc gccgcatggt ctcgaggcgc
     2401 cggccgacga ttgtctggat cgataatgtt gtccggttgg atccgg
//
LOCUS       DBNGSH010000965         4720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2677550, whole genome shotgun sequence.
ACCESSION   DBNGSH010000965 DBNGSH010000000
VERSION     DBNGSH010000965.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2677550"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..873
                     /gene="hslV"
                     /locus_tag="AC16CY_22085"
     CDS             349..873
                     /gene="hslV"
                     /locus_tag="AC16CY_22085"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR3356557.1"
                     /translation="MHATTILALRHQDRTVLAGDGQVTLGATVVKHSARKIRRIYNDT
                     ILAGFAGSAADSFALFSRFESKLEQYRGNLERASVELARDWRTDRLLRRLEAMLIVAD
                     RQSTFLLSGTGDLIEPDDGVVAVGSGGSYAMAAARALVRHSPLSARQIAEESMRIAAD
                     ICVYTNSNLTIEEL"
     gene            882..2270
                     /gene="hslU"
                     /locus_tag="AC16CY_22090"
     CDS             882..2270
                     /gene="hslU"
                     /locus_tag="AC16CY_22090"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR3356558.1"
                     /translation="MAIFLPERTTTAVGSLTPREIVTELDRYVIGQAKAKRAVAIALR
                     NRLRRQRLSPEMAEEVTPKNILMIGPTGVGKTEIARRLARLAHSPFIKVEASKFTEVG
                     YVGRDVESMVRDLVEVAVDMVRAEKSEEVRDKARAAAEERLLDLLLPPSRPSAPGEDP
                     AALRDQAGRTRDRMREQFRDGRLDERVVEIDVQAPAMPAFEIIAGSSVEEIGVNLKDM
                     MGNMFGKGARTRRLKVPAAFDHLLAEEQAKLVDTDSVNRTAVQRVEQTGIIFVDEIDK
                     IASRDGAGGGRGPDVSREGVQRDILPIVEGTTVNTKHGMVKTDHILFIAAGAFHVSKP
                     SDLIPELQGRFPIRVELEPLTRADFVRILTEPQNALVKQYSALLGTEDVALTFTPGAV
                     DRIADFAARVNDMTENIGARRLHTVMERLLDEVSFSAPELTEKTLTIDEAYVDRMLAD
                     IVRNEDLSRYIL"
     gene            complement(2301..2765)
                     /locus_tag="AC16CY_22095"
     CDS             complement(2301..2765)
                     /locus_tag="AC16CY_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356559.1"
                     /translation="MTDQRLHRLVHEGLRDSDTFRGLVARLEQSDVVVYFECDSAASP
                     AGRLTFVSAVAGVRYLHVRVSRLGSREQQIALIAHELRHAVEIADAPDVVDVASLGAA
                     YERIGYVNHRVATRGVAFDSEAAVRTGHQVLRELSGRQRSGPSLLATVKATR"
     gene            3171..>4720
                     /locus_tag="AC16CY_22100"
     CDS             3171..>4720
                     /locus_tag="AC16CY_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HIR3356560.1"
                     /translation="MPAARPGSDATVARDARRLSTLLEVSQALSGTLNFKSSIQRVLL
                     TLMRHHSVLRGMVTLLRDGELHVEAVEGFEDRARTIRIRLGEGITGKVVESGKPIVVP
                     RISKEPAFLNLAPRRPENPKQELSYICVPITLNRAAVGALAVDLRFQPDRDYDSSVKF
                     IGVVSSMIAQALNVQRMVEEERRRLLDENTHLRQELKERYDFSNIIGTSGPTRQMYEQ
                     VAQVAQTNTTVLIRGESGTGKELIAHAIHYNSLRVKKPFVKVSCAALPDTLIESELFG
                     YEKGAFTGAHTRKKGRFEMAEGGTLFLDEIGDINLGTQVKLLRVLQEREFERVGGTET
                     VKVNVRLIAATNKDMEKALVDGTFREDLYYRLNVFTIFVPPLRDRKADLLLLADHFLE
                     KFSREHGKVIKRISTPAIDMLMAYHWPGNVRELENALERAVLVCDGAVVHGHHLPPSL
                     QTAEASGTVTRVTLKDAVASYERDLIIDALKTTRGNRAKAARLLDTTERILNYKVRGY
                     GIDVRRFKT"
BASE COUNT          874 a         1561 c         1576 g          709 t
ORIGIN      
        1 gcgcggctcg gcccgacgct gctcggatcg accttgcccg ccttgcccgc ggtcgccgcc
       61 ccgctggcgc tggccgcctt ccgacgaagg gacgatggtt gacgacagca tcgcgaccac
      121 gaccacagcg accagagttg ggactcgcat acaggaggtt ggagcaacac tgctgccagc
      181 tggaaatgga cgatttcttt ccgaaataac ggcgtatcga ctgccaggcc cggcctgtgt
      241 cctcagctgt ttccagcgga caggacactc ggctgaaacc cgaatcctgc cccctgcccc
      301 ctgtcccctg acccccgccc cctgaccccc gcgaatatac tggccgtgat gcacgccacc
      361 acgatcctcg ccctgcgcca tcaggaccgg acggtcctcg ccggggacgg gcaggtcacg
      421 ctcggcgcga ccgtggtcaa acacagcgcg cgcaagatcc ggcgaatcta caacgacacg
      481 atcctcgcgg ggttcgccgg atcggccgcc gattcgtttg cgctcttctc gcgattcgaa
      541 tcgaagctcg aacagtaccg cgggaacctg gagcgagcct cggtggagtt ggctcgcgac
      601 tggcgaaccg accggctgct gcggcgactc gaagcgatgc tcatcgtcgc ggatcgccag
      661 tccacgttcc tgctctccgg cacgggcgat ctcatcgagc ctgacgacgg cgtcgtggcc
      721 gtcggatcgg gagggtcgta cgcgatggcg gccgcgcggg cgctcgtgag gcacagcccg
      781 ctgtcggcgc gccagatcgc ggaagagtcg atgcgcatcg ccgccgacat ctgcgtctac
      841 acgaattcga acctcacgat cgaggaactc tgacggacgg tatggcgatt ttccttcccg
      901 aacgcacgac gacggccgtg gggtcgctga caccgcgaga gatcgtcacc gagctggatc
      961 ggtatgtcat cggccaggcg aaggccaagc gggccgtcgc gatcgccttg cgcaaccgcc
     1021 tccgccgcca gcggctgtcc cccgagatgg ccgaggaggt gacgcccaag aacatcctga
     1081 tgatcggacc cacgggcgtg gggaagaccg agatcgcgcg acggctggcg cggctcgcgc
     1141 actcgccatt catcaaggtc gaggcgtcga agttcaccga ggtggggtac gtcggccggg
     1201 acgtcgagtc gatggtccgc gacctcgtgg aggtcgccgt cgacatggtc cgcgccgaga
     1261 agagcgaaga ggtccgcgac aaggcgaggg ccgccgccga ggaacggctc ctcgatctgc
     1321 tcctgccgcc gtcgcgtccg tcggcgccag gcgaggaccc ggcggccctc cgagatcagg
     1381 ctggccgcac gcgagatcgg atgcgggaac agttccggga cggccgcctc gacgagcgcg
     1441 tcgtggagat cgacgtgcag gcgcccgcga tgccggcgtt cgagatcatc gccgggtcct
     1501 cggtcgagga gatcggggtc aacctgaagg acatgatggg caacatgttc gggaagggtg
     1561 cgagaacgcg tcggctgaag gtgcccgccg ccttcgatca cctgttggcg gaggagcaag
     1621 ccaagctcgt ggacacggac tcggtcaacc ggaccgccgt gcagcgcgtc gagcagacgg
     1681 gcatcatctt cgtggacgaa atcgacaaga tcgcgagccg ggacggcgcg ggcggcggcc
     1741 gggggccgga cgtgagccgc gagggggtgc agcgcgacat cctgccgatc gtcgaaggca
     1801 cgacggtcaa cacgaagcac gggatggtga aaaccgacca tatcctgttc atcgcggccg
     1861 gcgccttcca cgtctcgaag ccgtcggatc tcatcccgga gctgcagggc cggttcccga
     1921 ttcgcgtcga gctcgagccg ctgacccgcg cggacttcgt ccggatcctg accgagcccc
     1981 agaacgccct ggtcaagcag tactcggcgc tgctcggcac cgaggacgtg gcgctgacgt
     2041 tcacgccggg ggccgtcgac cggatcgccg acttcgcagc ccgcgtcaac gacatgacgg
     2101 agaacatcgg agccaggcgc ctccatacgg tgatggagcg tctgctcgac gaggtctcct
     2161 tcagcgcccc ggagctgacc gagaagaccc tgacgatcga cgaagcctac gtagaccgca
     2221 tgctcgccga catcgtcagg aacgaggatc tgtcgcgcta catcctgtag ctcccgaggg
     2281 ccacgacccc tcggcgacgg ctaccgggtg gccttcaccg ttgcgaggag actcggaccg
     2341 gaccgctggc gtcctgacag ctcgcgcagc acctggtgcc ccgttcgcac cgctgcctcg
     2401 ctgtcgaagg cgactccacg tgtcgcgact cgatgattga cgtacccgat ccgttcgtac
     2461 gccgccccca gtgacgccac gtcaaccacg tcgggcgcgt cggcgatttc cacggcgtga
     2521 cggagctcat gagcgatgag cgcgatctgt tgctcccggg atccgagccg cgacacgcgc
     2581 acgtgcaggt atcgaacccc cgccacggcg gacacgaacg tgaggcggcc agcggggctc
     2641 gcggcgctgt cgcattcgaa atagacgacg acgtcggact gctcgagacg cgcgacgaga
     2701 cctcgaaacg tgtcagagtc gcggagcccc tcgtggacga gtcgatgcag cctttggtcg
     2761 gtcatcctga cgtgcgcatg cgcatcgtcg ggaggggact cggcggcgag gggtgctgcg
     2821 gaaccgaggg cgaacactgt gaggaggcca accgcgatcg gtgagaacgt catcgaaggc
     2881 gcagaatggc aaggcgcgag ccacgcgcac gcggggcgca ttcacgccga atcgctcgct
     2941 ttcctgcgga attccggtac aatggggctg ctgaactcag tcgagaaagg ccacagattc
     3001 gtggtgtgac tgtgctgtaa ctacaaaaaa gtcatgatcg ggggaacgtc gattcccccg
     3061 gccgcaggcc ccaagacgcg ctggcgggta gattcgccgc tccacgtccc gagcgacgac
     3121 agctttggtt cgaacgccga atcagtgcgc agaaatgtag gagacctctt atgcctgccg
     3181 ccagacccgg atccgacgcg accgtggcgc gcgatgcgcg ccgcctctcg accctgctcg
     3241 aagtcagcca ggccctgtcg ggcacgctca acttcaagtc ctccattcag cgcgtcctcc
     3301 tcaccctgat gcgccaccac agcgtgctcc gcggcatggt gacgctgctg cgcgacggcg
     3361 agctccacgt cgaggcggtc gaggggttcg aggatcgcgc ccgtaccatc cgcatccgcc
     3421 tcggcgaggg catcacggga aaggtcgtcg agagcggcaa gccgatcgtc gttccccgga
     3481 tcagcaagga gccggcgttc ctcaacctgg cgccgcggcg gccggagaac ccgaagcagg
     3541 agctgagcta catctgcgtg ccgatcacgc tcaatcgcgc ggccgtgggg gcgcttgcgg
     3601 tcgatctgcg cttccagccc gatcgcgact acgacagcag cgtgaagttc atcggcgtcg
     3661 tcagctcgat gatcgcccag gcgctcaacg tccagcggat ggtcgaggaa gagcggcgga
     3721 ggctgctcga cgagaacacg cacctgcggc aggagctgaa ggagcgctac gacttctcga
     3781 acatcatcgg cacgagcggc ccgacccggc agatgtacga gcaggtcgcg caggttgccc
     3841 agaccaacac cacggtgctc atccgcggtg agtcgggcac cggcaaggag ctgatcgcgc
     3901 acgcgatcca ctacaactcg ctgagggtca agaaaccgtt cgtgaaggtg agctgcgcgg
     3961 cgcttcccga cacgctcatc gagtcggagc tgttcgggta cgagaagggc gcgttcacgg
     4021 gagcccacac ccgcaagaag gggcggttcg agatggcgga ggggggcacg ctctttctcg
     4081 acgagatcgg cgacatcaac ctgggcaccc aggtgaagct gctccgcgtg ctgcaggagc
     4141 gggagttcga gcgcgtgggc gggaccgaga cggtgaaagt aaacgtccgg ctgatcgccg
     4201 cgacgaacaa ggacatggaa aaggcgctcg tggacggcac cttccgcgag gacctgtact
     4261 accggctcaa cgtgttcacg attttcgtgc cgccgctgcg cgaccggaaa gcggacctgc
     4321 tgctgctcgc cgaccacttc ctcgagaagt tctctcgcga gcacggcaag gtcatcaagc
     4381 gcatctcgac acccgccatc gacatgctga tggcgtatca ctggcccggc aacgtccgcg
     4441 agctcgaaaa cgccctcgag cgggcggtgc tcgtctgcga cggcgccgtg gtgcacggac
     4501 atcacctgcc tccttcgctg cagacggccg aagcgtctgg aaccgtgacg cgcgtgacgc
     4561 tgaaggatgc cgtggccagc tacgagcggg acctgatcat cgacgcgctc aagaccacgc
     4621 gaggcaaccg cgccaaggcg gcgcggctgc tcgacaccac cgagcggatc ctcaactaca
     4681 aagtacgggg atacggcatc gacgtgcggc ggttcaagac
//
LOCUS       DBNGSH010000966         2878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1998799, whole genome shotgun sequence.
ACCESSION   DBNGSH010000966 DBNGSH010000000
VERSION     DBNGSH010000966.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1998799"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="AC16CY_22105"
     CDS             <1..240
                     /locus_tag="AC16CY_22105"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3356561.1"
                     /translation="VTIQAPDESGTLQRWSIEWAGTAALDNQGVKRDALRVGDAVVIV
                     GRPSRVPGEYRALMVSLKRPLDGFAWGARTGEIVD"
     gene            233..985
                     /locus_tag="AC16CY_22110"
     CDS             233..985
                     /locus_tag="AC16CY_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356562.1"
                     /translation="MTRLPVIVVAALVLAAPIAAAAQRGRGGAPAAPQAARDAAPIDL
                     TGYWVSVVTEDWKLRMVTPRKGVYDTLTLNPAGRKVGDAWDPAKDEAAGEQCRAYGAP
                     AIMRIPGRLHITWQDANTLKIDTDAGTQTRLFRFGAPQPSAEPSWQGDSTAQWQLGPA
                     RRGGPRTGSLKVVTNNLRTGYLRKNGAPYSKDATVTEYYDLNSFPNGDTWFTVTTKVE
                     DPVYFTRAYITTTDFKKLPDASGWNPTACSSR"
     gene            1085..1966
                     /locus_tag="AC16CY_22115"
     CDS             1085..1966
                     /locus_tag="AC16CY_22115"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR3356563.1"
                     /translation="MRASGAGLGCPDWPRCFGSWIPPSSPADLPPQFDPSLFNPTLMW
                     TEYLNRLLGVSVGLLILATVVSAWRHHRHEPRILWSSVAALLLTGFQGWLGGRVVAHE
                     LAAWIVTVHMIVALVIVQLLLYATVATRPHGAATTGTPRTPVTRATLSLILVTMIQIA
                     IGTQVRGAVDVALDGGVPRAGAVASVGMLDHLHRGAALAVFAGSLMMVLWLGSRVPGE
                     RTLIRWAYVVAGLATAQVGLGVVMTYVSLLPAAQVGHLVVASVLLGAETVLLLLGRTE
                     SVAAAFPLRDRRLPRVP"
     gene            complement(1935..>2878)
                     /locus_tag="AC16CY_22120"
     CDS             complement(1935..>2878)
                     /locus_tag="AC16CY_22120"
                     /inference="COORDINATES: protein motif:HMM:NF017591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR3356564.1"
                     /translation="VSARASLSGTIRNCAGGPTPWNTWLSCEESLVGPRDRSPMPRQH
                     GYVFEVPLTGASNAEPIKAMGCFVHEAAAVDPRTGFVYETQDRGQAGLYRFVPNTPGQ
                     LAKGGRLEILAVDGRQRFDTSRGQRAGVSYPIHWEAIRDPDRAHHNGTDGLGVLQQGL
                     DRGGATFSRLEGAWFGDGRLFVTATDGGDARMGQVWELDPERSILRLVFESTGAPELN
                     MPDNITVSPRGGLVLCENGAKAVQHVHGLTRDGRVFRFARNNVRLNSERNGLRGDFTM
                     TEFAGATFSPDGRWLFFNVQVPGITCAVTGPWEDGGL"
BASE COUNT          424 a          975 c          980 g          499 t
ORIGIN      
        1 gtcacgattc aggcgcccga cgagagtggt acgctgcagc gatggtccat cgagtgggcc
       61 ggcactgcgg cgctcgacaa ccagggcgtc aagcgcgacg cgctgcgggt cggcgacgcg
      121 gtcgtcatcg tcggccgtcc atccagggtg cccggcgagt atcgcgcgct gatggtcagc
      181 ctcaagcggc cgctcgacgg cttcgcgtgg ggcgcgcgga cgggtgagat tgttgactag
      241 actccccgtc attgtcgtcg cggcgctggt cctcgcggct ccgatcgctg cggccgcgca
      301 gcggggaaga ggcggcgcgc cagccgcccc gcaggccgca cgcgacgccg cgccgatcga
      361 tctgacgggc tactgggtct ccgtcgtcac cgaggactgg aagcttcgga tggtgacgcc
      421 caggaagggc gtctacgaca cgctcacgct caaccccgcg ggccggaagg tgggcgacgc
      481 gtgggacccg gccaaggacg aggcggccgg cgagcagtgc cgggcctacg gcgccccggc
      541 catcatgcgc atccccggac ggctgcacat cacgtggcag gacgcgaaca cgctcaagat
      601 cgataccgac gccgggacgc agacgcgcct cttccgcttc ggggcgccgc agccgtctgc
      661 cgagccgtcg tggcagggcg actcgaccgc tcaatggcag ctcggaccgg cgcggcgagg
      721 ggggcctcgt accgggagct tgaaggtcgt gaccaacaac ctgcggacgg gctacctgcg
      781 caagaacggc gcgccctaca gcaaggacgc cacggtgacc gagtactacg acttgaactc
      841 cttccccaac ggtgacacct ggttcaccgt cacgaccaaa gtcgaagatc ccgtctactt
      901 cacgcgggcg tacatcacga cgaccgattt caagaagctt ccggacgcgt ccggctggaa
      961 tcctacggcc tgctcgtccc ggtagtaagc aagtagcagg cagctggctg tagtctcttc
     1021 tttccatcgt ctcgccacct ggacgacggc ggccacctat ttcctgatcc tcgtcggcgg
     1081 gctcgtgcgt gcgtcgggcg caggactggg ttgcccggat tggccgcgct gcttcgggtc
     1141 gtggattccg ccctcttcac cggcggacct gccgccgcag ttcgatccct cgctcttcaa
     1201 ccccaccttg atgtggacgg agtacctcaa ccgcctgctc ggcgtgagcg tgggcttgct
     1261 gatcctcgca acggtggtgt ccgcctggcg gcatcatcga cacgagccgc ggatcctgtg
     1321 gagctcggtt gcggcattgt tgctgacggg atttcaaggc tggctcggcg gccgcgtggt
     1381 ggcgcacgag cttgccgcgt ggatcgtcac ggtgcacatg attgttgcgc tcgtgatcgt
     1441 gcagctgttg ctgtacgcga cagtagcgac ccgcccccat ggagccgcca cgacgggcac
     1501 cccgcgcacg ccagtcaccc gagccacctt gtccttgatc ctcgtgacga tgatccagat
     1561 cgccatcggg acccaggtcc gcggcgcggt tgatgtggcg ctcgacggcg gcgttccgcg
     1621 cgcgggcgcc gtcgcctcgg tggggatgct cgatcacctc caccgcggcg cggctctcgc
     1681 tgtcttcgcg ggctcgctga tgatggtgct ctggctcgga tccagggttc ccggcgaaag
     1741 gaccctcatc cgctgggcct atgtggtcgc gggtcttgcg acggcccagg tcgggctggg
     1801 cgtcgtgatg acgtatgtgt cgctcttacc ggctgcgcag gtcgggcatc ttgtggtcgc
     1861 gagcgtgttg ctcggcgctg aaaccgtgtt gctgttgctc ggcaggaccg aaagcgtggc
     1921 ggcagcgttc ccgctcagag accgccgtct tcccagggtc ccgtaacggc gcaggtgatg
     1981 ccgggcacct gcacgttgaa gaacagccag cggccgtcgg ggctgaaggt ggcgcccgcg
     2041 aattccgtca tcgtgaagtc tccgcgaagg ccgttccgct cgctgttgag cctgacgttg
     2101 ttgcgcgcga aacggaagac ccgcccgtcg cgcgtcaggc cgtggacgtg ctgcaccgcc
     2161 ttggctccgt tctcgcacag cacgagaccg ccgcgcgggc ttaccgtgat gttgtcgggc
     2221 atgttcaact ccggcgcgcc cgttgactcg aatacgagcc ggaggatgct ccgctccgga
     2281 tcgagctccc acacctgtcc catccgtgcg tccccgccat cggtggccgt gacgaacagg
     2341 cgcccgtccc cgaaccacgc gccttcgagc cgcgagaagg tcgcgcctcc acggtcgagg
     2401 ccctgctgaa gcacgccaag cccgtcggta ccgttgtgat gggcccgatc gggatcgcgg
     2461 atggcttccc agtgtatggg gtaggagact cccgcacgct gaccacggct cgtgtcgaac
     2521 cgctgtctgc cgtcgacggc gagtatctcc aaccggccgc ctttggccag ctgccctggt
     2581 gtgtttggaa cgaagcgata gagtccggcc tgcccgcgat cttgcgtctc gtagacgaag
     2641 cccgtccggg gatcgacggc ggcggcctcg tgcacgaagc atcccatcgc cttgatcggc
     2701 tcggcattcg aggcgccggt cagtggaact tcgaagacgt atccgtgctg gcgcggcatg
     2761 ggactgcggt cgcggggccc gaccagcgac tcctcgcacg acagccacgt gttccacggc
     2821 gtcgggccgc cggcgcagtt gcggatcgtg cccgagaggc tggcgcgcgc gctcacca
//
LOCUS       DBNGSH010000967         3730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_302056, whole genome shotgun sequence.
ACCESSION   DBNGSH010000967 DBNGSH010000000
VERSION     DBNGSH010000967.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_302056"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="AC16CY_22125"
     CDS             complement(<1..397)
                     /locus_tag="AC16CY_22125"
                     /inference="COORDINATES: protein motif:HMM:NF042769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3356565.1"
                     /translation="MTRHQIGRRLSMAALLAVMGFVLAGSPAGQTPAVREHKDLVYAT
                     VDGKSLGLDLYMPAGVQSPGLVVWVHGGAWRNGTKAQVQKQFFENGYATASLDFRQST
                     EARFPAQAHDIKAAIRFLRAKAKEYGYRTD"
     gene            521..1696
                     /locus_tag="AC16CY_22130"
     CDS             521..1696
                     /locus_tag="AC16CY_22130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3356566.1"
                     /translation="MHLSRRSSHLRPTAVNTILAEIRQAQARGRTIVSLMRGEPDFET
                     PPHIAEAAARAIQAGRTRYPDNRGEPSLREAVAAKLSRDNGVTYDPASEILVTTGATL
                     GIYAALMALLNEGEDVLLADPVYDAYHSPIVLAGGRPVPVPATIEDGRFVITVAAIEN
                     ALTTRSRALLLNTPWNPVGTVFRASELQAIGDIVLRRDLALISDEIYEAITYGDARHV
                     SPSSLSGELRARTILVNSLSKTYAMTGWRVGYCAAPAPVIDSMFLVLQQSSRGPSTFV
                     QDAAAAALSGPQSSVNAMRDEYARRRSQVLSALAGIKRVRVLPPEGGFFAMVDARDTK
                     IPSDEIRRRLLNKSGVAVVHGAAYGRAGEGTLRVSFASGGDTLTRGLERLRAGLKAL"
     gene            1693..>3730
                     /locus_tag="AC16CY_22135"
     CDS             1693..>3730
                     /locus_tag="AC16CY_22135"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR3356567.1"
                     /translation="MSTELARALADERIEDVRFDSVSRALYSTDASVYQIEPVGVALP
                     RSRDELRRIVQVCARLGCPITMRGGGTSQAGQAVGHGLVVDTSKHLNAVLEVNAAERW
                     ARVEPGVVLDELNAQLRQHGLRFAPDISTASRATIGGMMANNSSGARSIVYGKTIDHV
                     IEQDVVLSDGSVAVFGPVTDRQLSDKRAGDSLEARCYRLVSELAQRHREEIDRRFPKV
                     LRRVGGYNLDEFTPGRPFNLAKLMVGSEGTLGVVLSAKLNLVPLPRAKAVLAIQFHHL
                     LESLAATPLILRHSPSAVEVMDKSILDHTRKSAALEALRRSFIEGDPAALLCVEFYGD
                     RAEDLPPRLDALEHALRESGLGYRFFHALDLSQQARIWSLREAGLGLSMAMRDDAKSI
                     SFVEDTAVAPERLRDYIERFLAIVAHHGTTAGVYAHASVGCLHVRPVVNMKTEAGVRQ
                     FEAIANDISDLVLEFGGALSGEHGDGLVRGPFMAKMFGPALYDAFRTIKATFDPTGIF
                     NPGKIVDAPPITANLRFGPRYAARNPPTYFDYAEGGLAGAIEMCSGLGACRKTLEGTM
                     CPSYMATREEQHSTRGRANVLRLTIAGRLGESGLGDAGVYDTLDLCLECRACKAECPV
                     GVDVARFKSEFLADYWQRHGLSREARVLGHARTVAAWGSRLAPLSNWVAASRAVR"
BASE COUNT          600 a         1289 c         1221 g          620 t
ORIGIN      
        1 gatccgtccg gtacccgtac tccttggcct tcgcgcgcag gaagcggatg gcggccttga
       61 tgtcgtgggc ctgggccggg aagcgcgctt cggtggactg gcggaagtcc aggctcgccg
      121 tcgcgtatcc gttctcgaag aactgtttct ggacctgcgc cttcgtgccg ttgcgccagg
      181 cgccgccgtg cacccagacg acgagcccgg gcgactgcac gcctgccggc atgtacagat
      241 ccaggcccag gctcttcccg tcaaccgtcg cgtagaccag atccttgtgc tcgcgaaccg
      301 ccggcgtctg gcccgcggga gacccggcga gcacgaagcc catgacggcc aggagcgcgg
      361 ccatcgacaa gcggcgtcca atctgatgtc tggtcatcgg cgtgccctca cagttcatcg
      421 tcacgcggaa ccagccgcgc tcggtggagt gtagaacatc gcactttcca cttcaaacgt
      481 acttttgaac tttgaacctt ccactttaga cttgcagacc ttgcaccttt cacggcgctc
      541 ctcgcaccta cgccccactg ccgtcaacac gatcctcgcc gagattcgac aggcgcaggc
      601 tcgagggcgg accatcgtgt cgctgatgcg cggcgaaccc gactttgaaa cgcctcctca
      661 catcgccgag gcggccgctc gtgccataca ggcgggtcgt acgcgctacc cggacaaccg
      721 tggagagcct agcctccgcg aggcagtagc tgcgaagctc tcgcgggaca acggcgtgac
      781 ctatgacccc gcgtctgaaa tcctcgtgac gaccggagcg acgcttggca tctatgcggc
      841 cctgatggcg ctcctgaacg agggagaaga cgtgctgctg gcggacccgg tctatgacgc
      901 ctaccactcc cccatcgtgc tcgcaggcgg ccggccggtt cccgtgcccg cgaccatcga
      961 agacgggcgg ttcgtcatca ccgtcgctgc catcgagaac gcgctcacga cccgctcgcg
     1021 tgcgctgttg ctgaacacac cctggaaccc ggtcggcacg gtgtttcgag cgtcggagct
     1081 tcaggcaatc ggcgacatcg tcctgcgccg cgatcttgcg ctcatcagcg acgagatcta
     1141 cgaggcgatc acctacggcg atgcgcggca cgtctcacca tcctcgctgt cgggcgagct
     1201 gcgcgcgcgc acaatcctcg tcaacagtct gtcgaaaacg tacgcgatga ccggatggcg
     1261 cgtgggttac tgcgccgcgc cggcgcccgt catcgacagc atgttcctcg tgctgcagca
     1321 gtcgagccgt ggaccgtcca cgttcgtcca ggacgcggct gcggccgcgc tgtcgggtcc
     1381 ccagtcgtcg gtgaacgcaa tgcgcgacga atacgcgcgc cggcgatcgc aggtgctgag
     1441 cgctctcgcg ggcatcaagc gggtgcgcgt gcttccaccc gagggcgggt tctttgcgat
     1501 ggtggacgcg cgagacacga agatcccttc cgacgagatc cggcgccggc tcctgaacaa
     1561 gtccggtgtc gccgtcgtcc acggcgccgc ctacggacgc gcaggcgaag gcacgcttcg
     1621 cgtctcgttc gcgagcggcg gcgacacgct cacccgaggc ctggaacggc tgcgtgccgg
     1681 gctcaaggcg ctgtgagcac cgaactggcg cgggcgctgg cagacgagcg catcgaagac
     1741 gtccggttcg acagcgtgtc ccgggcgctc tactccaccg atgccagtgt ctaccagatc
     1801 gagccggtcg gcgtggcgct cccacggtcg cgcgacgagc tcaggcggat cgtgcaggtc
     1861 tgcgcgcggc tcggctgccc catcacgatg cgcggagggg gcacgtcaca ggccggccag
     1921 gccgtcggtc acggcctcgt cgtcgatacg tcgaagcacc tgaacgccgt cctcgaggtg
     1981 aacgcggccg agcgatgggc gcgtgtcgag ccgggcgtcg tgctcgacga gctcaacgcg
     2041 cagctccgtc agcacggctt gaggttcgcg cccgacatct ccacggccag ccgcgcgacc
     2101 atcggcggca tgatggcgaa caactccagc ggcgcgagat cgatcgtgta cggaaagacc
     2161 atcgaccacg tgatcgagca ggacgtcgtg ctgtcggacg gctcggtcgc cgtcttcggc
     2221 ccggtcacgg atcgccaact gtccgacaag cgcgccggcg actcgctcga ggcccgctgc
     2281 taccgtttgg tctctgaact ggcccagcgg cacagggagg aaatcgatcg gcgcttcccg
     2341 aaggtgctgc ggcgcgtggg gggctacaac ctcgacgaat tcacgcccgg ccggcccttc
     2401 aatctggcca agctgatggt cggctccgaa ggcacgctcg gcgtcgtgct ctccgcgaag
     2461 ttgaacctcg tgccgcttcc gagggccaag gccgtcctcg ccatccagtt tcaccatttg
     2521 ctggaatcgc tggccgccac gccgctcatc ctccgccact cgccctcggc cgtcgaagtg
     2581 atggacaagt cgatcctcga ccacaccagg aagagtgccg cgctcgaggc gctcaggcgc
     2641 agcttcatcg agggcgatcc tgctgcgctg ctttgcgtgg agttctacgg agaccgcgcc
     2701 gaggatctgc cgccccggct cgatgcgctc gaacatgcgc tgcgcgagag cggcctgggc
     2761 taccgctttt tccacgcgct cgacctcagc cagcaggccc gtatctggag ccttcgcgag
     2821 gcaggtctcg ggctctccat ggcgatgcgc gacgacgcca agtcgatctc gttcgtcgaa
     2881 gacaccgccg tcgcgccgga gcggctgcgc gactacatcg agcgcttcct cgccatcgtc
     2941 gcccatcacg ggacgaccgc cggcgtctac gcccatgcgt cagtgggatg cctgcacgtg
     3001 cggccggtcg tcaacatgaa gaccgaagcg ggcgtcaggc agttcgaggc catcgcaaac
     3061 gacatctcgg atctggtgct cgagttcggg ggcgcgctgt cgggcgaaca cggtgacggc
     3121 ctcgtgcgcg gcccgttcat ggccaagatg ttcgggcccg cgctgtacga cgcgtttcga
     3181 acgatcaagg cgacgttcga tccgacgggg attttcaatc ccggcaagat cgtcgatgcg
     3241 cctcccatca ccgccaatct tcgattcggg ccccgttacg ccgctcggaa tccgccgacc
     3301 tacttcgact acgccgaagg cggcctggcg ggcgcgatcg agatgtgcag cggtctcggt
     3361 gcgtgccgca agaccctcga agggacgatg tgcccttcct acatggccac gcgcgaggaa
     3421 cagcattcga cgcgagggcg ggccaacgtg ctgcggctca cgattgccgg acggctcggc
     3481 gagtcgggcc tcggcgacgc cggcgtgtac gacacgctcg acttgtgcct cgagtgccgc
     3541 gcgtgcaagg ccgagtgccc cgtgggcgtg gacgtggccc gcttcaagag cgaattcctc
     3601 gcggactact ggcagcggca cggcctgtcg cgagaggccc gcgtgctcgg ccacgcgcga
     3661 acggtcgcgg cctggggcag ccgtctggcg ccgctctcga actgggtggc ggcaagccgc
     3721 gccgtccgaa
//
LOCUS       DBNGSH010000968         5906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1490346, whole genome shotgun sequence.
ACCESSION   DBNGSH010000968 DBNGSH010000000
VERSION     DBNGSH010000968.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1490346"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /gene="smpB"
                     /locus_tag="AC16CY_22140"
     CDS             complement(<1..302)
                     /gene="smpB"
                     /locus_tag="AC16CY_22140"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR3356568.1"
                     /translation="MASDKTDREKAQRTIADNRKALHDYHVIDTWEAGIVLLGTEVKA
                     IREASVNLRDSYARIEHGEVWLMNVHISPYSHTGYAHHDERRQRKLLLHRHEIQKLT"
     gene            complement(1616..2065)
                     /locus_tag="AC16CY_22145"
     CDS             complement(1616..2065)
                     /locus_tag="AC16CY_22145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3356569.1"
                     /translation="MPITADVVLGARVTIVHPGLVNLYGCRIGDDTKVGASIGERCKI
                     SSHSFICEGVTIEDEVFVGHGVMFINDPRPRATSEGRLRMEADWHVVPTRVARGASIG
                     SGAAILCGVTIGEHAMVGAGAVVTRDVPPYATVAGVPARARGSGSAR"
     gene            complement(2070..3509)
                     /locus_tag="AC16CY_22150"
     CDS             complement(2070..3509)
                     /locus_tag="AC16CY_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356570.1"
                     /translation="MKILFHVNLRTMLRHFESVVLELAARGHIVRIASTADRKDVPPP
                     DALLGHERISFVEASDERSDAWRERIVEFRVWRDYLRYLDKRFEQAPKLRARVVRKLA
                     AAITHDERTHLVAFCPHCEGRLVDNDVGVILRTGLSKKGFNNLRSLFALMEETIPSDP
                     GIDALLRQEQPDVLVITPLIKLASRQPDFVKSARALGIPVVYPVFSWDNLSTKGLVHV
                     QPDAVLVWNHRQFAEAVEMHHVPGDRVVVTGAPRFDEFFAMTPQTSRDEFCRQHGLDA
                     RQPIVSYLCSSEFVAGHEFEFVQRWIEEIRLAPALRRCNVLIRPHPRRTKSWKGFKTA
                     DRQIAVAMPRRMNGDQTLFDTVHHSAAVVGLNTSAELEAGIVGRPVLTVLAPESAGGQ
                     RGTLHFDYLLKEHGGFVQLAPDMDTHRRQLSVAVAGDYDAAAIRAFVRDFLRPHGLER
                     PVTPIVADAIEAAARQTSGTGQPLAAQHG"
     gene            3739..5682
                     /locus_tag="AC16CY_22155"
     CDS             3739..5682
                     /locus_tag="AC16CY_22155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356571.1"
                     /translation="MTTSVTPRVRSEVPVGRRGLLVGTSVAVAGTFLFRFLTVAFTND
                     HFVHLSRGWQILQGDIPDIDFFDEGVLGPYYASAAALAWSGHNLFGEAIVTVAFIAAA
                     AGLTFLASARLSGSLWLAAAATTAAVISMPRLYGYPKVFFYVLAIVAAWRYAHRPGRG
                     NLVWLAVVTVVAFLFRHDHGVYIGVATVAMLTVLHWGQTRDVVAALTRYSAVTAVMLL
                     PFLVFVQVTVGLLRYTGDLPAPSLRFNLTRFQIDPSAPWVVVPPPSGPRVNVRWRDDI
                     DDAARRDLERRHDLLDPRHVEDSTWSYVPANIDESHLHALVRDPAAADTHGIDRAAYA
                     ITEPPPLYLRLQRRFPVLRAQVAPGIFSPGNAFSWFYYVTFLLPLLGLLLVGLLLRSG
                     RISRPEAAVACMASVLCLIVVQQLVRGSPDSRLPDVANATAVLGAWVTARLLDAGVGL
                     GRLARRAWTLAVAAAGIATLWSVWTFAEASASLETSRLLTGPAGVWKRMQAVSTNLHT
                     RPIDNWTRESPGLGGLVRYVFECTSATDRLLVTWFAPEVFFQAERAFAGGQVYLMPRW
                     HSSPADQRLTIERLEGQRVPIVLEKAGEDYASYFPLVADYLRGRYRSAPIQGESMRDF
                     RVLVDTQLTPTGTYEPLGTPCYR"
     gene            5682..>5906
                     /locus_tag="AC16CY_22160"
     CDS             5682..>5906
                     /locus_tag="AC16CY_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356572.1"
                     /translation="MIAPSAVSVSRPPVGGPLFVAGIAVAVVGTFLFRFLTADFTNDH
                     YVHLSRGWQILQGDVPTRDFFDPGLVLQYYT"
BASE COUNT          973 a         2014 c         1952 g          967 t
ORIGIN      
        1 gtgagcttct ggatctcgtg acggtgcagg agcagcttgc gctgccggcg ctcgtcgtga
       61 tgggcgtagc cggtgtggct gtaggggctg atgtggacgt tcatcagcca gacctcaccg
      121 tgctcgatcc gggcgtagct gtcgcggagg ttgaccgagg cctcgcgtat cgccttcacc
      181 tcggtgccga gcaggacgat gccggcctcc cacgtgtcga tcacgtgata gtcgtgaagg
      241 gccttgcggt tgtccgcaat ggtgcgctga gccttctcgc gatcggtctt gtcgctggcc
      301 acggtccggc gcgaatcgta ccacgcgcac gtgggacggc agatcgacga cgaccaggcc
      361 gaacagggcg atggcggccc cgtgggccca cgggcggctc cgccccgcga ccagtctcac
      421 cagccgatcg acgccggtgg cgccgagcat cgccaccgcg agggtcacga cgaacagcat
      481 ccgcccggga atccgaagac ccggaaagaa caggtagtgc atccggtaga acggcagcac
      541 acctgccagg acgaatgcga tgaagccgag cgccagtatc gcgaacagga tggcgacacg
      601 gcgatgatcg cggccgatga aggcgaaggg cacggccagc gccaggaccc agccgacgta
      661 cgcggtgtga tcgcccatga accgcagggg ggattcgacg ggaatgccag agaacgggaa
      721 gaagaacgtt cccagatcgc gaagcaacca tgcaccgcgc accgcagtcg aaaacggcac
      781 gccaccggtg cgggccgcct cgctcaccag ctgcaacgag gggagcagct cgaacgacgc
      841 caggccggcg gcgaggatgc cggcgatgcc gagttgcgcg atcggcagca gccgcgccga
      901 accacgccgg ccgtccgacg ggaacagcgc cagccagagc atacataccg ctgccgccgc
      961 ggccacatag atcgttccct ggaggtaccc ggccaggaac tgcaccacca gaacgatcac
     1021 cagtgcgggg tgcggccacg gcgtcgatcg tcgggccgaa tagatgatca ggccgaggac
     1081 ccacggcagc cacgcggtcg caaacagcag cagcaggtga ccgttgtaga tccatgccgg
     1141 gacgcttccc ccgagcgtga atgcgacggc agcggccgcc gccgaagtcc atcgcaatcc
     1201 gatgacgcga gtcaggaacg ccgtgccgac gccggcgatc cacatgtgca gcgccatcat
     1261 ccacgaaaag aacgcggcgg gcgggaacca ccgcagcagc atcgccggcg ggcacaggac
     1321 cgtcgtctcg gtgtcggcga ggtgtggcgt gccgccgaag aggaacggat ccaatgtggc
     1381 agccggccaa gcgacagcac ggaccgcgca tacagctccc acccatacag gttgctggcg
     1441 tcggtgcctg acagcacgcc cgcacgcgat ccgaagagag ggcggagcgt gaagagcacc
     1501 agcgctgcga ccggcacggc aacgatcgcg aggccgcgat cgcagatcgt caacgaggtc
     1561 gctcctagcg gggagtcgga gctcgtgtcc agggcacctc gcctggtcgg ctcactcatc
     1621 gcgcggaccc gctcccgcga gcacgcgccg gcacacccgc caccgtcgca tacggcggca
     1681 catcgcgcgt gacgacggcg ccggcgccga ccatcgcgtg ctcaccgatc gtgacgccgc
     1741 agaggatggc cgcgccgctg ccgatggatg ctccccgcgc gacgcgggtc ggcaccacgt
     1801 gccagtcggc ctccatccgc aggcgaccct ccgaggtcgc gcggggccgc ggatcgttga
     1861 tgaacatcac gccatggccg acgaagacct cgtcttcaat cgtgacgcct tcacagatga
     1921 agctgtgtga cgagatcttg cagcgttctc cgatggaagc gccgaccttg gtgtcgtcgc
     1981 cgatccggca gccgtacagg ttcaccaggc cgggatggac gatcgtgacg cgtgctccga
     2041 gaacgacgtc ggccgtgatt ggcatcggct caaccgtgct gcgcggccag cggctggccg
     2101 gttccagacg tctgccgcgc cgcggcttca atcgcatcgg ccacgatcgg cgtgaccggg
     2161 cgctcgagcc cgtgcggacg aaggaagtca cgcacgaacg cccggatcgc ggcggcgtca
     2221 tagtcgccag ccactgccac tgacaactgc cgacggtggg tgtccatgtc gggcgccagc
     2281 tggacgaagc cgccatgctc cttcagcagg tagtcgaagt gcagcgtgcc gcgctgccca
     2341 cccgccgact cgggtgccag tactgtcagc accggcctgc cgacgatgcc cgcttccagt
     2401 tcggcgctgg tgttcaagcc gacgaccgcc gcgctgtggt ggacggtgtc gaagagcgtc
     2461 tggtcgccgt tcatcctccg tggcatggcc accgcgattt gccggtccgc ggtcttgaat
     2521 cccttccagg atttcgttcg gcgcggatgc ggccggatca gcacgttgca gcggcgcagc
     2581 gccggcgcca ggcggatctc ctcgatccaa cgctggacga attcgaattc gtgcccggcc
     2641 acgaattcgg acgagcagag ataactgacg atcggctggc gagcgtccag gccgtgctgc
     2701 cggcagaatt cgtcgcgcga ggtctgcggc gtcatggcga agaactcatc gaagcgcggc
     2761 gcacccgtga cgacgaccct gtcgccgggg acgtggtgca tctcgaccgc ttcggcgaat
     2821 tgacgatggt tccacaccag caccgcgtcg ggctgcacgt gcaccagccc cttcgtcgac
     2881 aggttgtccc agctgaagac gggatagacg accggaattc cgagcgcgcg tgcgctcttg
     2941 acgaagtcgg gctgacgcga tgcgagcttg atgagcgggg tgatgaccag cacatcgggc
     3001 tgctcctgcc gcagcagcgc gtcgattccg gggtcgctcg ggatcgtctc ctccatcaat
     3061 gcgaacaggc tgcgcaggtt gttgaagccc ttcttgctca acccggttcg caggatgacg
     3121 ccgacatcgt tgtcgacgag tcggccctcg caatgcggac agaacgccac caggtgcgtc
     3181 cgctcgtcgt gggtgattgc ggcggcgagc ttacgaacca cccgcgcccg cagcttcggc
     3241 gcctgctcga agcgcttgtc gagatagcgc agatagtcgc gccatacgcg gaactcgaca
     3301 atccgctccc tccaggcgtc actgcgctcg tcggaagcct cgacgaacga gatgcgctcg
     3361 tgtcccagga gcgcgtccgg cggcggaacg tccttccggt cggcagtcga cgcgatgcgc
     3421 acgatatgac cgcgggccgc cagttcgagt acgacgctct cgaaatgcct gagcatcgtc
     3481 cgcaggttga cgtgaaagag gatcttcaca agcggaacgt gctccagcca agagacgata
     3541 ggaacgatta acggagtttt cgtgcgcctg gttgacggaa tggccgttat gcggccccag
     3601 tcctcccccg gcacagcgcg gattgccccc acgcacggcg cggatcatcg tcccgcagaa
     3661 tgcacttgtc aaacgagcac gtgggaatcg taccacgcgg ttgacccgcg caagaccggt
     3721 tcatatactc gcaaacccat gacgacgtcg gtcacgcctc gtgtccgaag cgaggtgccg
     3781 gtcggccgcc ggggcttgct cgtcggcacg agcgtcgccg ttgcagggac gtttctcttc
     3841 cgattcctca ccgtcgcgtt caccaacgat cacttcgtgc acctctcgcg cgggtggcag
     3901 atccttcagg gggacattcc cgacatcgac ttcttcgatg agggcgtctt ggggccgtac
     3961 tacgcatcgg ctgccgcgct cgcctggtcg gggcacaacc tcttcggtga agcgatcgtc
     4021 acggtggcct tcatcgccgc cgcggccggg ctcacctttc tggcgtccgc acgcctgtcc
     4081 ggctcgctgt ggctcgctgc cgccgcgaca acagccgcgg tgatctcgat gccgcggctg
     4141 tacgggtacc ccaaggtctt cttctacgtg ctcgcgatcg tcgccgcctg gcgctacgcg
     4201 catcgacccg ggaggggcaa tctcgtctgg ctcgccgtcg tcacggtcgt ggcgttcctc
     4261 ttccgtcacg accacggcgt ctacatcggc gtggccaccg tcgccatgct cacggtgctg
     4321 cactggggac agacgcggga cgtcgtggct gcgctcacga gatacagcgc ggtgacggcg
     4381 gtgatgctgt tgccgtttct cgtcttcgtg caggtgacgg tcgggttgct ccgctacacg
     4441 ggcgacttgc ctgcgccgtc gcttcggttc aacctgacgc ggtttcagat cgatccgtcg
     4501 gcaccgtggg tcgtcgtgcc gccgccgtcg gggccgcgcg tgaacgtgcg gtggcgggac
     4561 gacatcgacg acgccgcgcg ccgggatctc gagcgccggc acgatctgct ggatccccgg
     4621 cacgtggaag actccacgtg gagttatgtg ccagccaata tcgacgagag ccacctccac
     4681 gcgctcgtcc gcgatcccgc tgccgccgat acgcacggta tcgatcgggc cgcatatgcg
     4741 atcacggaac cgccaccgct ctacctgcgc ctgcagcgcc ggtttcccgt gctgcgcgcg
     4801 caggtcgcgc cggggatctt ctcgccggga aacgctttct cgtggttcta ctacgtcacg
     4861 ttcctgctgc cgctgctggg acttcttctc gttggcctgc tgctgcggag cggccgcatc
     4921 agccggcccg aagccgcagt ggcctgcatg gcgagcgtgc tctgcctcat cgtcgtccag
     4981 cagctcgttc gcggctcgcc cgattcacgg cttcccgacg tggcgaacgc gaccgccgtt
     5041 ctcggcgcct gggtgaccgc caggctcctc gacgccggtg tcggactcgg ccggctcgcg
     5101 cggcgcgcgt ggaccctggc cgttgctgcc gctgggatcg ccacgctgtg gagcgtctgg
     5161 acgttcgcgg aggcgtcggc gagcctggaa accagccggc tcttgaccgg ccccgccggt
     5221 gtgtggaagc gaatgcaggc ggtctcgacg aacctgcaca cgaggcccat cgacaactgg
     5281 acacgcgagt cccccggcct gggagggctc gtccgctacg tcttcgagtg cacatccgcc
     5341 accgaccgcc tgctcgtcac gtggtttgcg ccggaggtgt tcttccaggc cgagcgcgcg
     5401 ttcgccggcg gccaggtcta cctgatgccc cgctggcaca gctcgcccgc ggaccagcgc
     5461 ctcaccatcg agcggctcga ggggcagcgc gtgccgattg tgctcgagaa agccggcgag
     5521 gactacgcaa gctacttccc gctcgtggcc gactacctgc gcggccgcta caggtccgcg
     5581 ccgatccagg gcgagtccat gagggacttt cgcgtgctcg tcgatacaca actgacgccg
     5641 actggcacgt acgagccgct cggcacgccg tgctaccggt gatgatcgcg ccgtcggctg
     5701 tctccgtcag caggccgccg gtcggcgggc cgttgttcgt cgccggcatc gcggttgcgg
     5761 tcgtcggcac gtttctcttc cgttttctca ccgccgactt caccaacgat cactacgtcc
     5821 atctgtcgcg cgggtggcag atcctgcagg gcgacgtccc cacgcgggac ttcttcgatc
     5881 cgggactcgt cctgcagtac tacacc
//
LOCUS       DBNGSH010000969         3765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2849138, whole genome shotgun sequence.
ACCESSION   DBNGSH010000969 DBNGSH010000000
VERSION     DBNGSH010000969.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2849138"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="AC16CY_22165"
     CDS             <1..1434
                     /locus_tag="AC16CY_22165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356573.1"
                     /translation="MNTRAAALIAFVILPSVTASGQTWTPPRTPWGDPDLQGDYSNKY
                     EQGTPFERPAEFEGRTIDDIKGDELARLVKERAVEVLLNSPFTGGDPVAGNFGGAPAF
                     YDRFEADRGSRPWFVVDPPDGRIPPMTPEGQQASAARAAARAAARRGRGPADSYIDRS
                     LYDRCITRGLPGSMMPANYGNSYRIVQGPGFVAISYEMVHETRVIPLDGRPRLGSAIR
                     QYMGEGRGRWEGDTLVVETTNFRDEPVYRGSNPARLRLIERFTPIARDRILWSVTVDD
                     ASTWTRPWTFAMPLTRSEGEAIFEYACHEGNRAMANLLSAARTEEKTGARATSESRTP
                     PPSREGANPGSRVSALAGDWRIAGTGGRGNFAGYSTPTRLTIQESAAEVTIDTDTGTE
                     NQMQSATYRLDGADTAVPGPLGWDTKANAARREDALVVTITRTIDGPDGKLRFEIRDT
                     YRVSGDVLTLERTQGSQSRKMTYNRVR"
     gene            complement(1437..2861)
                     /locus_tag="AC16CY_22170"
     CDS             complement(1437..2861)
                     /locus_tag="AC16CY_22170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide isomerase"
                     /protein_id="HIR3356574.1"
                     /translation="MKACLIAVAGSLLGASTLYAADPPTFYKDVLPVLQANCQGCHRP
                     GEVAPMSLVTYEQARPWARAIKAATSSRQMPPWFADPSYGHFANERRLVDREIAMLGA
                     WADAGAPAGNEADAPAPRTFQNGWNITPDLIVEMPKPFELPARGTINYKYILVKTNFP
                     QDMWVTSAEMRPGNAEVLHHGKVWVRPQGSKWMENAVPGEAYEVESHRDILGRNAIEE
                     GNDILGKFNPGLGAQPFDIEGAAKFVPKGSDLVFELHYTTSGKPTSDVSKLGLVLAKA
                     PPEKRYFFHAGPTALNLAIPPGEGAAEVVSEITFGEDAQLVYAQPHMHLRGKDFELRI
                     VSPSKETTTVLKGNWDFEWQIGYQYAEPILLPKGSRLQLISHFDNSPANRFNPDPTKK
                     VVWGPQNWDEMSNCFIGVLFKTDTAPEKVFLRSGPSMLPRGDSGPTLAALNQAPDAVA
                     PATNGNINANVSPATNGGPGSFER"
     gene            2994..3170
                     /locus_tag="AC16CY_22175"
     CDS             2994..3170
                     /locus_tag="AC16CY_22175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356575.1"
                     /translation="MEFRCPTCGAESALRLPADAFPTHVTLKCLSCAALVRLPRNRPD
                     FSESPGPDGAGTAI"
     gene            3203..3649
                     /locus_tag="AC16CY_22180"
     CDS             3203..3649
                     /locus_tag="AC16CY_22180"
                     /inference="COORDINATES: protein motif:HMM:NF016535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3356576.1"
                     /translation="MNNLYLLLALATGAGVAVMAVLNARLGVLLGGPFWAAAAQFTVA
                     LSAVLTIALLTRQPAPATAGLGGAPWWVWAGGLFSATFIVVSILLTPRLGVALTLATI
                     IVGQLTAALVVDHYGLFGASVIRVSLPRVLGVLLLLVGITLLRWRP"
BASE COUNT          604 a         1249 c         1252 g          660 t
ORIGIN      
        1 atgaacacac gtgccgccgc cctgatcgcg ttcgtgatcc tgccctccgt taccgcgtca
       61 ggccagacgt ggacgccgcc ccgcacgccc tggggcgatc ccgacctgca gggcgactac
      121 tcgaacaaat acgagcaggg caccccgttc gagcggccgg cggagttcga aggccgcacg
      181 atcgacgata tcaagggcga cgagctcgcc cggcttgtca aggaacgcgc cgtcgaagtg
      241 ctcttgaact ctccgttcac gggcggcgat cccgtggccg gcaacttcgg cggcgctccg
      301 gcgttctacg accggttcga agccgatcgc ggcagccggc cgtggttcgt cgtcgatccg
      361 cccgacggca ggatcccgcc aatgacgccg gaggggcagc aggcgtcggc cgcccgcgcg
      421 gcagcacgcg ctgcggcgcg tcgagggcgc ggtccggccg attcctacat cgatcgaagc
      481 ctctacgacc ggtgcatcac ccgcggcctt ccgggatcga tgatgcccgc caactacggc
      541 aactcctatc gcatcgtcca ggggcccgga ttcgtcgcca tttcgtacga gatggtgcac
      601 gagacgcgcg tcatcccgtt ggacggccgc ccacggctcg ggtcggccat ccgtcagtac
      661 atgggcgagg ggagaggccg ttgggaaggc gacacgctcg tggtcgagac gaccaacttc
      721 agggacgagc ctgtctaccg cggctcgaat cccgcgaggc tgaggctgat cgagcgcttt
      781 acaccaatcg cgcgcgacag gattctgtgg tcggtgaccg ttgacgatgc ctcaacgtgg
      841 acgcgtccct ggacgttcgc catgccgctg acgcggagcg agggcgaggc catcttcgag
      901 tacgcgtgcc acgaggggaa cagggcgatg gcgaatcttc tcagcgctgc gcgcaccgag
      961 gagaagacgg gcgcgcgcgc gacaagcgaa tcccgaaccc cgccgccttc gcgcgaaggg
     1021 gcgaatcccg gatcccgagt ctcagcactc gcaggagact ggcgcatcgc cggcaccggc
     1081 ggccgcggga atttcgccgg gtactcgacg cccacccgat tgacgattca ggagtcggcc
     1141 gccgaggtca cgatcgacac cgataccggc accgaaaacc agatgcagtc ggcgacctac
     1201 cggctcgacg gggccgacac agcggtgccc ggaccgcttg gatgggatac gaaggcgaac
     1261 gctgcccgcc gtgaggacgc gctcgtcgtg acgatcacgc ggacgatcga tgggcctgac
     1321 ggcaagctgc gcttcgagat cagagacacc taccgagtga gcggtgacgt gctcacgctc
     1381 gagcgaacgc agggaagcca gtcgcggaaa atgacctaca accgcgtgcg gtagccttac
     1441 cgctcgaagc tcccagggcc tccgtttgtc gccggcgaga cgttggcgtt gatgttgcca
     1501 ttggtggccg gcgcaacggc gtccggcgcc tggttgagcg ccgccagcgt cggccccgag
     1561 tcgccgcgtg gcagcatgct cggccccgat ctcaggaaca ctttctcggg cgccgtgtcg
     1621 gtcttgaaca gcacgccgat gaagcagttg ctcatctcat cccagttctg cggcccccac
     1681 acgactttct tggtcggatc cgggttgaag cggttggcgg gcgagttgtc gaagtgggag
     1741 atgagctgca gcctcgagcc cttgggcagg aggatcggct cggcgtactg atagccgatc
     1801 tgccactcga aatcccagtt gcccttcagc accgtcgtcg tctccttcga gggcgacacg
     1861 atgcgcagtt cgaagtcctt gccgcgcagg tgcatgtgcg gctgtgcgta gacgagttgg
     1921 gcgtcctcgc caaaggtgat ctcgctcacg acttcggccg ctccctcgcc cggagggatc
     1981 gccaggttca gcgcggtcgg cccggcatgg aaaaaatagc gcttctcggg cggcgccttc
     2041 gccaggacga gtccgagctt ggacacgtcg gaggtcggct tgccagacgt cgtgtagtgc
     2101 agctcgaaca cgaggtccga tcccttcggg acgaactttg ccgccccctc gatgtcgaag
     2161 ggctgcgctc cgaggcccgg gttgaatttg ccgaggatgt cgttcccttc ctcgatcgcg
     2221 ttgcgtccca ggatgtcgcg atggctttcg acctcgtagg cctcaccggg aaccgcgttc
     2281 tccatccact tcgatccctg cggacgcacc cacaccttgc cgtggtgcag cacttcggcg
     2341 ttgcccggac gcatctccgc cgacgtcacc cacatgtcct gcgggaagtt cgtcttcacg
     2401 aggatgtact tgtagttgat cgtccctcgc gccggcagct cgaacggctt cggcatctcg
     2461 acgatgaggt ctggcgtgat gttccagccg ttctggaacg tgcgaggcgc tggcgcgtcg
     2521 gcctcgttgc cggccggcgc tccggcatcg gcccacgccc cgagcatggc gatctcgcgg
     2581 tcgaccagcc ttcgctcgtt ggcgaagtgg ccgtagctcg ggtcggcaaa ccacggcggc
     2641 atctgcctgc tggatgtggc ggccttgatg gcgcgcgccc atggcctggc ctgctcgtag
     2701 gtcacgagcg acatcggcgc cacttcgccc ggccggtggc agccctgaca gttcgcctgg
     2761 aggacgggaa ggacgtcctt gtagaaggtc ggagggtcag ccgcgtacag cgtcgatgct
     2821 ccgagcagag aacccgcaac ggcgatgaga cacgccttca tagtcagcac tcctggcttc
     2881 acacggtcga tacggcccca gtatacagtc tggagggcct ttccgatgga aaacgcccgg
     2941 cggctcatgt gaaaatgatg acacttcgga acatgtcgag aagccccgca atcatggaat
     3001 tccgctgtcc gacctgcggc gccgagtcgg cgctgcggct cccggcggac gcgtttccca
     3061 cccacgtcac gctcaagtgc ctctcgtgtg cggcactcgt ccgtctgccc aggaacaggc
     3121 cggacttttc cgagtcgcca ggccccgacg gagccggcac ggctatctga ggtatcttca
     3181 tcgctctggc agcggagcga ttgtgaacaa tctgtacctg cttctggcgc tggcaaccgg
     3241 agctggcgtc gcggtgatgg cggtactcaa cgctcgactg ggcgtgctcc tcggcggacc
     3301 gttctgggcg gcggctgcgc agtttaccgt cgcgctgagc gccgtgctca cgatagcgct
     3361 gttgacgcga cagccggccc ccgcaaccgc aggactcgga ggcgcgccgt ggtgggtgtg
     3421 ggccgggggt cttttcagcg cgacgttcat cgtcgtgagc atcctgctga cgccgcgtct
     3481 cggcgtcgcg ctgacgctcg ccaccatcat cgtcggacag ctcacggcgg cgcttgtcgt
     3541 cgatcactac ggcctcttcg gcgcatcagt catccgcgtc tcgctgccac gggtcctggg
     3601 ggtgctgttg ttgctggtcg gcatcacgct cctcaggtgg aggccgtagg tgcgcgattc
     3661 gcagcgccgt ctgctcaccg agctccggca ggcgctggtg ccgctccacc ggacgctgct
     3721 cgaatgggag cgcgcggcct acgagcgggt gcacggtcgg acggg
//
LOCUS       DBNGSH010000970         6367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2000429, whole genome shotgun sequence.
ACCESSION   DBNGSH010000970 DBNGSH010000000
VERSION     DBNGSH010000970.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2000429"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="AC16CY_22185"
     CDS             <1..312
                     /locus_tag="AC16CY_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356577.1"
                     /translation="PWSGYNPGTGAGSRAVEIVTPAVSSPPMFSSAPLRDAAKLLLAA
                     AGHVKFLDGDSRGYVLLDIGPDRMLADWYFVPTVTERTDRESRAAGFVCDRGSSRLVR
                     Q"
     gene            complement(320..733)
                     /locus_tag="AC16CY_22190"
     CDS             complement(320..733)
                     /locus_tag="AC16CY_22190"
                     /inference="COORDINATES: protein motif:HMM:NF014471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR3356578.1"
                     /translation="MNPLVVYVLLLKAVATSFAGMGSLPQIHEDFVETRRVLTDEQLS
                     RAVLVGRSTPGPVGAYVVAVGYFAAGWRGAVAGWLAMITPALAAIPLFTLMQRWLHVA
                     RVRAAVDAVVIASAVLLLSSAFQLAVDGFRQLLPR"
     gene            complement(730..1284)
                     /locus_tag="AC16CY_22195"
     CDS             complement(730..1284)
                     /locus_tag="AC16CY_22195"
                     /inference="COORDINATES: protein motif:HMM:NF014471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR3356579.1"
                     /translation="MNTISHFGSSVARDANRTLGGGWAAIELLRRTFIASGALDEEGH
                     AGLVAVSRLTPGTNLLAYCVALGWRMHGWRGSLTALGAASVPGSVIVFALTAALVRVD
                     QYSLVRFALAIGILAATVLVLSAAWSLLRPYLTASGLASTSVIIALSAALLLAGLTPV
                     RILLLSAAAGAMLGRPLRAPQEEP"
     gene            1395..3563
                     /gene="ppk1"
                     /locus_tag="AC16CY_22200"
     CDS             1395..3563
                     /gene="ppk1"
                     /locus_tag="AC16CY_22200"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR3356580.1"
                     /translation="MDTPRDSVVVASRARTQPVRRDAPAKPAIPSDRSTDPADLKNPA
                     LYINRELSWLEFNERVLAQARDASHPLLERVKFLAITGTNLDEFFMIRVATTLKKLRE
                     DIDDVAADGYNTEQQLDAMRARARKMLADQAATWADLRRQLEAEKIFFLETDAWTPAV
                     RDYLSGYFSREICPVLTPLAFDPGHPFPFISNLSRNFAVVVRHAARTKFARVKVPDVL
                     PRFISLPPSVSPSDGTAFVFIEDVIRANIQELFPGTQVRGAYQFRVVRDTDLEIEEDE
                     ADDLLETIEGSLRQLRRGAISLLHVEAGMPTRVLNILAENFEVTDDVVLRTQDRLGFG
                     DWWQLTKLHRPELKDSPFSQRSPWRSDEDPEVIFDQIRYQDILVHRPFESFASLETFL
                     RAAVKDPHVTAIKITLYRIGADSPLIPLLVAAAEAGKQVAVLVELKARFDERNNILWA
                     RQLESHGIHVVYGFANLKTHAKLCLVVRQEAAGIQRYVHTSTGNYNPDTAKVYTDLSL
                     FTADPDIVADASDVFNYLTGYSNQKEFRSLIVAPVQLRQRLKEMIEREMEHARAGRPA
                     RLMIKVNAITDDQMIRVLYRAAQAGVSMDLLVRGICCLRPGVPGVSDTIRVRSIVGRF
                     LEHSRLFWFYNNGDEEMYIGSADLMERNLDRRVESLAPIRDPEIREHLRDIVLGAYLR
                     DTERAMVLDAAGHYERPAVESERFDAQQFLIRHYTDIGES"
     gene            complement(3798..3986)
                     /locus_tag="AC16CY_22205"
     CDS             complement(3798..3986)
                     /locus_tag="AC16CY_22205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356581.1"
                     /translation="MRTALTIYAIGVLVGLWRTDGPPATKLLVALLWPIGPLAFLVTV
                     SGLLVAAVIAFAGPGRRA"
     gene            4035..5336
                     /locus_tag="AC16CY_22210"
     CDS             4035..5336
                     /locus_tag="AC16CY_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3356582.1"
                     /translation="MRIAGPVILAEVGWMSMGLVDTIMVGPLGPAAIAATGMGSSVFT
                     AIAIFGMGLMLGLDAFVSQAHGAGDERECTRWLHQGVWLACLAAVPVMALTWLAFATL
                     DLWGLHPDIRRLVGPYLSVISAGALPLLLYAAFRRYLQGMHVVRPIMIALVSANLVNA
                     AVNWVLIYGNLGMPALGVVGSAWATTAARIYMALFLYAAIRLDNRRRGRQDRGVYIRV
                     DWHRIWRLVRLGFPAASQVALEVGVFAAATALAGRLDPLSSGSHQIALNVASLAFMVP
                     LGLSSAAAVRVGFAFGAGDLQRAARAGWTALAAGAVIMTAIGLLFFFWPVPLLSVFTT
                     DARIIEIGVGLLAIAAAFQIFDGTQAVATGALRGISETRMPMIVNVIGHWLLGLPVGY
                     ALCFLLGWGVAGLWIGLSIGLVLAALVLTGVWWRRTLHDLR"
     gene            5323..6234
                     /locus_tag="AC16CY_22215"
     CDS             5323..6234
                     /locus_tag="AC16CY_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3356583.1"
                     /translation="MICDSHCHFFSTQFLAALSRQRGRGDTVASLCGELQWDDPAEPE
                     ALADRWVKELDERGVARSALIASIPGDEGSVASAVARHPGRFVGFFMIDPSAEDAPDR
                     VRHAVATLGLRVVCLFPAMHHVALTDPRIERIVDVAVSLPGTAIFVHCGVLSVGVRKK
                     LGLASRFDLRLGDPLGVARLALTFPRVPFIIPHFGAGLLRETLMAADASANVYVDTSS
                     SNSWIRYAPELTLARVFEAAVSVLGPSRLLFGTDSSFFPRGWQRGVYESQRAAAAAAG
                     ITTGDQALIFGGNFERLFPSQSPSPRV"
BASE COUNT         1016 a         2159 c         2126 g         1066 t
ORIGIN      
        1 ccgtggtccg gttacaaccc gggcacaggc gcgggatcgc gggcggttga aatcgtcacg
       61 ccggccgtga gctcgccgcc gatgttttcg agcgcgcccc tgcgcgacgc ggcgaagctc
      121 ctgctggcgg ctgccggcca cgtgaagttc ctcgacggcg acagccgggg ctacgtcctg
      181 ctggacatcg gaccggatcg catgctggcg gactggtact tcgtgccgac ggtcaccgag
      241 cggaccgacc gtgaatcgag agcggcgggc ttcgtctgcg accgggggtc gtcgaggcta
      301 gtacggcagt agactgccgc taacgcggta gtagctgccg gaatccatcg accgccagct
      361 gaaacgcaga cgacaggagg agaaccgcgc tggcgatgac aaccgcgtcg acggctgcac
      421 gaacacgggc aacgtggagc cagcgctgca tcagcgtgaa gagcgggatc gccgccagcg
      481 ccggcgtgat catggcaagc cagccggcca ccgcgccgcg ccaccccgct gcgaagtacc
      541 ccacagcgac gacgtaggcg ccaaccggac ccggcgtgga ccggccaacg aggacggccc
      601 ggctcagttg ctcgtcggtc aggacccgcc gcgtctcgac gaagtcctcg tgaatctggg
      661 gcagcgagcc catgcctgcg aacgacgtgg cgaccgcctt cagaagcagc acgtagacga
      721 cgagcgggtt cacggttcct cctgcggcgc gcgcagcggc cggcccagca tcgctccagc
      781 ggccgcggac agcagcagga ttctcacggg cgtcagaccc gcgagcagca gcgccgcgga
      841 cagggcgatg atgacgctgg tacttgcgag cccggacgca gtcaggtacg ggcgcaggag
      901 actccacgcg gccgagagca cgagcaccgt cgccgcaagg attccaatcg ccagtgcaaa
      961 tcgtacgagc gagtactgat cgacccgcac caatgcggcg gtcaaggcaa agacgatgac
     1021 cgagccggga accgatgcgg ccccgagggc ggtgagcgat cccctccacc cgtgcatgcg
     1081 ccatccgaga gcgacgcagt aggccagcag gttcgttcct ggagtcaggc gcgagacggc
     1141 tacgagtcct gcgtgtccct cttcgtcgag cgctcccgag gcgatgaacg tgcgccgcag
     1201 gagttctatc gcggcccagc cgccaccgag cgttctgttg gcatcgcgag caacggacga
     1261 cccaaagtga gaaatagtgt tcatttctgg cattcgaccg tggcagcaga ttctctcagt
     1321 tttggcccaa atcctggcgt catttggctc tgatatactc aaaatcttgc ggcctgagcg
     1381 gccccaggcc ccctatggac accccccgag attccgtcgt cgtcgcatcg cgtgcacgga
     1441 ctcaacccgt acggcgggac gccccggcca aaccggccat accgtcggat cgctcgacgg
     1501 atccggccga cctcaagaac cccgcgctct atatcaaccg ggaactgagc tggctcgaat
     1561 tcaacgagcg tgtgctcgcg caggcgcgtg acgcctccca cccgctgctc gagcgggtga
     1621 agttcctcgc gatcaccggc acgaacctcg acgagttctt catgattcgc gtcgccacga
     1681 cgctgaagaa gctccgcgag gacatcgacg acgtcgcggc ggacggctac aacacggagc
     1741 agcagctcga cgccatgcgc gcgcgggcgc ggaagatgct ggccgatcag gcggccacgt
     1801 gggccgatct gagacggcag ctcgaggccg aaaagatctt cttcctcgag accgacgcgt
     1861 ggaccccggc ggtccgcgat tacctgagcg ggtacttttc gcgggagatc tgccccgtgt
     1921 tgaccccgct ggctttcgac ccgggccatc cgttcccgtt catctcgaac ctgagccgga
     1981 acttcgcggt cgtggtccgt catgcggcgc gcaccaagtt cgcgcgcgtg aaggtgccgg
     2041 acgtcctgcc gcgcttcatc agcctgcccc cgtcggtcag cccgtccgac ggcacggcgt
     2101 tcgtgttcat cgaggacgtg attcgcgcca acatccagga gctgttcccg gggacgcagg
     2161 tcaggggcgc ctaccagttc cgcgtcgtcc gcgacaccga tctggagatc gaggaggacg
     2221 aggccgacga cctgctggag acgatcgaag gcagcctgcg gcagcttcgc cgcggcgcca
     2281 tctcgctgct gcacgtcgag gccggcatgc cgacgcgcgt gctgaacatc ctcgcggaga
     2341 acttcgaggt caccgacgac gtcgtgttga ggacgcagga tcgcctcggc ttcggcgact
     2401 ggtggcagct gaccaagctg caccggccgg agctgaagga ctcgcccttc tcgcagcgaa
     2461 gcccgtggcg gagcgacgaa gaccctgaag tgatcttcga tcagatccgg tatcaggaca
     2521 tcctggtgca ccgtccgttc gagtcgtttg cgtcgctcga gacgtttctg cgcgccgcgg
     2581 tgaaggaccc gcacgtcacg gcgatcaaga tcacgctcta ccggatcggc gccgattcac
     2641 cgctcatccc gttgctcgtc gcggccgcgg aggcaggcaa gcaggtggcg gtcctcgtgg
     2701 agctcaaggc gcgttttgac gagcggaaca acatcctctg ggcgcggcag ctcgagtcgc
     2761 acgggatcca tgtcgtctac ggcttcgcca atctcaagac gcacgccaag ctgtgcctgg
     2821 tcgtccggca ggaggcggcc ggcatccagc ggtacgtcca caccagcacc ggcaactaca
     2881 acccggacac ggcgaaggtc tacaccgacc tcagcctctt caccgccgat cccgacatcg
     2941 tcgccgacgc gtcggacgtc ttcaactacc tgacgggcta ctcgaaccag aaagagttcc
     3001 gctcgctcat cgtggcgccg gtgcagctcc ggcagcggct gaaggagatg atcgagcgcg
     3061 agatggagca cgcacgggcc ggccggcctg cgcggctgat gatcaaggtc aacgcgatca
     3121 ccgacgacca gatgatccgc gtgctgtacc gggcggccca ggccggggtg tcgatggacc
     3181 tgctggtgcg cgggatctgc tgcctgcggc ctggcgtgcc cggcgtcagc gacacgatcc
     3241 gcgtgcggtc gatcgtcggc cgtttcctcg agcactcgcg gctgttctgg ttctacaaca
     3301 acggcgacga ggaaatgtac atcggcagcg cggatctgat ggagcgcaat ctcgaccggc
     3361 gcgtcgaatc gctggcgccg atccgcgacc cggaaatccg ggagcacctg agggacatcg
     3421 tgctgggcgc ctacctgcgc gacaccgagc gcgcgatggt gctcgatgcc gcgggccatt
     3481 acgagcggcc cgccgtcgag tccgaacgct tcgacgcgca gcagttcctg atccgccact
     3541 acaccgacat cggcgaatcc tagagccttt caaagcggac gccgtttcgt tcttggtcct
     3601 tcgtcacttg gttcttggtc cgtccctggt ccctggtccg tcctgggtcc ttggtcccag
     3661 gtccgccgag ccgagtccct cacgctgtgg cagctcgttc accagaggac aaaggacaaa
     3721 ggaccaagga cttgggacgg accaaggact caggacggac caagtcccaa gaaccaagga
     3781 ccaaggactg agaggcgcta ggctcttcgt ccgggtccgg cgaaggcgat caccgccgcc
     3841 accagcagac cgctcaccgt gacgagaaac gccagcggtc cgatcggcca cagaagggcg
     3901 acgagcagct tcgtggctgg cgggccgtcg gtccgccaga gtcccaccag cacgccaatc
     3961 gcgtagattg tcagagccgt gcgcattggt gtgacgctac tgtactgcga atgctcagcg
     4021 aactccgccc cctgatgcga atcgccgggc ccgtgatcct ggccgaagtg ggctggatga
     4081 gcatgggcct ggtcgacacg atcatggtgg gcccgctcgg ccccgcggcg atcgcggcga
     4141 cgggcatggg cagcagcgtc ttcacggcca tcgccatctt cgggatgggg ctgatgctgg
     4201 ggctcgacgc gttcgtgtcg caggcccacg gcgcgggcga cgagcgggag tgcacgcggt
     4261 ggctgcacca gggcgtgtgg ctggcgtgcc ttgccgcagt gccggtgatg gcgctgacat
     4321 ggctcgcatt cgcgacactg gacctgtggg ggctgcaccc cgacatccgg cggctcgtcg
     4381 ggccctatct gagcgtcatc tccgcgggcg cgctaccgct gctgctctac gccgcgtttc
     4441 gccggtacct gcagggcatg cacgtcgtcc ggcccatcat gatcgcgctc gtgtcggcga
     4501 acctcgtgaa cgcggcggtg aactgggtgc tcatctacgg caacctgggc atgccggcgc
     4561 tcggcgtcgt ggggtcggcg tgggccacga cggccgcgcg gatttacatg gcgctgtttc
     4621 tgtacgcggc catacgcctg gacaatcgac gccgcggccg ccaggaccgc ggggtctaca
     4681 ttcgcgtcga ttggcaccgc atctggcggc tcgtgcgcct cggattcccg gccgcgtccc
     4741 aggtggcgct cgaggtgggc gtcttcgcgg cggccacggc gctggcgggc cgcctcgatc
     4801 ccctgtcgtc cggctcgcac cagatcgcgc tcaacgtcgc ctcgctcgcc ttcatggtgc
     4861 cgctcggcct ttcgtcggcc gcggccgtgc gcgtcgggtt cgcctttggc gcgggagatc
     4921 tccagagagc ggcgcgcgcc gggtggacgg cgctggccgc cggggccgtg atcatgacgg
     4981 ccatcggcct gctcttcttc ttctggcctg ttccgctgct cagcgtcttc acgaccgacg
     5041 cgcgcatcat cgagatcggc gtcggcctgc tggcgatcgc cgccgcattc cagatcttcg
     5101 acggcacgca ggctgtcgca accggggcgc ttcgggggat ctcggagacg cgcatgccga
     5161 tgatcgtcaa cgtcatcggt cactggctcc tcggcctgcc cgttggctac gctctgtgct
     5221 tcctcctcgg gtggggcgtg gccgggctct ggattggcct gtcaatcggc ctggttctgg
     5281 ccgcgctggt cctgaccggg gtatggtgga gaagaacgct tcatgatctg cgatagccat
     5341 tgccacttct tctccacgca attcctggcc gcgctgtccc gccagcgggg gcgcggcgac
     5401 acggtcgcct cgttgtgcgg cgagctccag tgggacgatc cggcggaacc cgaagcgctg
     5461 gcggatcggt gggtgaagga gctcgacgag cgcggcgtcg cgcgctcggc tctcatcgcg
     5521 agcatccccg gcgacgaagg atcggtcgca tccgcggtcg cgcggcatcc cggccggttc
     5581 gtcggcttct tcatgatcga cccgtcggcc gaggacgcgc ccgaccgcgt caggcatgcc
     5641 gtggccacgc tggggctgcg cgtcgtgtgc ctcttcccag cgatgcatca cgtggcgctc
     5701 accgaccctc ggatcgaacg gatcgtcgat gtcgcggtgt ccctgccggg tacggcgatc
     5761 ttcgttcact gcggcgtgct gtcggtcggg gtgcgcaaga agctcggcct cgccagccgg
     5821 ttcgatctgc ggctgggcga tccgctggga gtcgccaggc tcgcgctgac gttcccgcgc
     5881 gtgccgttca tcattccgca tttcggcgcc gggctgcttc gcgaaacgct catggccgcc
     5941 gatgcgtccg ccaacgtgta cgtggacacg tcgagctcga acagctggat tcggtacgcg
     6001 cccgagctca ccctcgcccg tgtcttcgag gccgccgttt ccgtgctcgg cccgtcgaga
     6061 ctgctcttcg gcactgactc gtcgttcttc ccgcgcggct ggcagcgcgg cgtctacgag
     6121 tctcaacgag ccgccgcggc tgcggccggc atcacgacgg gcgatcaggc gctcatcttc
     6181 ggaggcaatt tcgagaggct gttcccgagc cagtccccgt cgccccgcgt ctaagcagcc
     6241 aggactggcg ctggacgacc gtggctgaca ttcctggaac cattctgtcc gccgccgcaa
     6301 tcacgtggca catggccgat gcgggcggcg attccgacgt cgatctcatg agccgcgccg
     6361 ccgcggg
//
LOCUS       DBNGSH010000971         3874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3867535, whole genome shotgun sequence.
ACCESSION   DBNGSH010000971 DBNGSH010000000
VERSION     DBNGSH010000971.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3867535"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..497
                     /locus_tag="AC16CY_22220"
     CDS             12..497
                     /locus_tag="AC16CY_22220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3356584.1"
                     /translation="MPIASNVVLGERVKIVHPDLVNLYGCRVGDDSRIGAFVEIQKNA
                     TVGSRCKISSHTFVCEGVTIEDDVFVGHGVMFTNDLRPRATTDGKPQSEADWHVVPTL
                     VKRGASIGSGAAIICGITIGEGAMIGAGAVVTRDVGPGETVAGVPARLLRPGAGQERR
                     A"
     gene            494..1519
                     /locus_tag="AC16CY_22225"
     CDS             494..1519
                     /locus_tag="AC16CY_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR3356585.1"
                     /translation="MIGIGVVGYGYWGPNLVRNFSDLPTARVVSVCDRRPERLAIVER
                     RYPGVRVTIDAAALIADPAVDAVAIVTPVQHHFELAMLALRAGKHVLVEKPMASTSEQ
                     AARLIEEARLRSLVLMVDHTFVYTSAVAKLRELVRNGELGDVYYYDSVRINLGLFQHD
                     VNVLWDLAVHDLSIMDYVLGRQPTAVSATGLAHVAGQPENIAYMTMFFDGPLIAHVHV
                     NWLAPVKVRQTLLGGSRRMAVFDDLDAGEKIKVYDKGISVNPSPENVYQMLVGYRTGD
                     MWAPKLDVTEALSVEAAHFVDCIGSGRTPITDGEAGLRVVRLLEAATVSMAARGQLVT
                     VDSVVHS"
     gene            1563..2249
                     /locus_tag="AC16CY_22230"
     CDS             1563..2249
                     /locus_tag="AC16CY_22230"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3356586.1"
                     /translation="MDPARARTVNAVHGRQPVTPDSPDEIALSDRLRGTHSREELLDL
                     AARHALEQSEAGARMRRAVWRALAKRFGHGVRIGRAALATHPETFEIGDGVFIGEQAF
                     IQGRFDGRCVIGDHSWIGPQSYFDARDLEIEEYVGWGPGARVLGSTHTGLPIDVPIIQ
                     TDLVIKPVRICAWADIGVNAVVMPGVTVGRGAIVGAGAVVTRDVEPYSIVAGVPARFV
                     RRRSGGPGEP"
     gene            2246..3214
                     /locus_tag="AC16CY_22235"
     CDS             2246..3214
                     /locus_tag="AC16CY_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3356587.1"
                     /translation="MKIDGAKIVVTGGAGLMGSTTVDLLLTQHDPRAVVIFDNFSRGT
                     QSNIQAALRDPRVTLVEGDLRDRQAVARALAGADAVIHMAAVRIILCAADPRLAFDVM
                     CGGVFNVVEAAQAAGVQKIVAASSASVYGLADQLPTTETHHPYNDRTWYGASKMFMEG
                     LLRSFNEMYGLPYVALRYFNVYGPRMDVHGKYTEVLVRWMERMDAGQPPLILGDGTQT
                     MDFVYVEDVARANLLALASDVSDDVFNVATGIETSLNELAAALARAMGVRCTPEYGPE
                     RKINSVPRRLGSTEKAARVLGFKASVGLEEGLSRLVRWWRAERVRV"
     gene            3214..>3874
                     /locus_tag="AC16CY_22240"
     CDS             3214..>3874
                     /locus_tag="AC16CY_22240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3356588.1"
                     /translation="MDTIPVARPLLDEREAEAARRVILSGWVTQGPEVASFEREFAAA
                     VGAPHACAVSSCTTALHVALKACGVEPGDEVVTVSYSFIATANAIRYCGAMPVFVDVR
                     PDGFNIDPDAVEQAIGPRTRAVLVVHQFGMPCDLRAILGVAGRHGVPVIEDAACAVGS
                     EIEWNGRWERIGRPHGLVACFSFHPRKLLSTGDGGMLTTADAALDRQFRLLRHHGMSV
                     SD"
BASE COUNT          591 a         1296 c         1359 g          628 t
ORIGIN      
        1 agacagttga tatgccaata gcgtcgaacg tcgtgctcgg cgaacgcgtg aagatcgtcc
       61 accccgatct ggtcaacctg tacggttgcc gcgtcgggga cgactcgagg atcggcgcct
      121 tcgtcgagat ccagaagaac gccacggtcg gcagccggtg caagatctcg tcgcacacct
      181 tcgtgtgcga gggggtcacg atcgaagacg acgtcttcgt cgggcacgga gtgatgttca
      241 ccaacgatct gcgcccgagg gcgacgaccg acggcaagcc tcagagcgag gccgactggc
      301 acgtggtgcc cacgctggtg aagcgcggcg cctcgatcgg cagcggcgcc gcgatcatct
      361 gcggcatcac gatcggcgag ggcgcgatga tcggcgcggg cgccgtcgtc acccgcgacg
      421 tcgggcccgg cgagacggtc gccggcgtgc cggcgcggct gcttcgccct ggcgccggtc
      481 aggagcgccg cgcgtgatcg ggatcggcgt ggtcgggtac ggatactggg gaccgaatct
      541 cgtccgcaac ttctccgacc tgcccacggc gagagtcgtc agcgtgtgcg atcgccgccc
      601 cgagcggctg gccatcgtcg agcgccggta tcccggcgtg cgcgtgacga tcgacgccgc
      661 cgcgctcatc gccgatcccg ccgttgacgc cgtggcgatt gtcaccccgg ttcagcatca
      721 cttcgagctg gcgatgctcg cgctccgcgc ggggaagcac gtgctcgtcg aaaagccgat
      781 ggcgtccacc tccgagcagg ccgcccgcct catcgaggag gcccggctcc gcagcctggt
      841 gctgatggtc gatcacacct tcgtctatac cagcgccgtc gccaagctgc gcgagctggt
      901 ccggaacggc gagctgggcg acgtctacta ctacgactcg gtgcgcatca acctcggctt
      961 gttccagcac gacgtcaacg tgctgtggga tctcgccgtg cacgacctgt cgatcatgga
     1021 ctacgtgctc ggccggcagc ccaccgcggt gtcggcaacg ggactcgccc acgtggctgg
     1081 tcagcccgag aacatcgcct acatgacgat gttcttcgac ggcccgctca tcgcgcacgt
     1141 gcacgtcaac tggctggccc ccgtcaaggt ccggcagacg ctgctcggcg gcagccggcg
     1201 gatggcggtg ttcgacgatc tcgacgccgg cgagaagatc aaggtctacg acaaggggat
     1261 ctcggtcaac ccgagccccg agaacgtcta tcagatgctc gtggggtatc gcacgggcga
     1321 catgtgggcg ccgaagctcg acgtcaccga agcgctcagc gtcgaggctg cgcactttgt
     1381 cgactgcatc ggctccgggc gcacgccgat caccgacggc gaagccggct tgcgcgtcgt
     1441 ccgcctgctc gaagccgcca ccgtgtcgat ggcggcacgg gggcaactcg tgacggtcga
     1501 cagtgtggtt cactcatgag cgtgccgaga gccggactgg ccgcgggagc gagggcctaa
     1561 gcgtggatcc ggcccgcgct cgtaccgtca atgccgtgca cggccgccag ccggtgacgc
     1621 ccgattcgcc ggacgagatc gcgctgtccg atcgcctgcg cgggacgcac tcgcgcgagg
     1681 aactgctcga cctggccgcc cggcacgcgc tcgagcagag cgaggccggc gcccgaatgc
     1741 gccgcgccgt gtggcgcgcg ctcgcgaagc ggttcgggca cggcgtccgg atcgggcgcg
     1801 cggccctggc gacgcacccc gaaacgttcg agatcggcga cggggtcttc atcggcgagc
     1861 aggcgttcat ccagggccgg ttcgacggcc ggtgcgtgat cggcgaccac agctggatcg
     1921 gcccgcaaag ctacttcgac gcccgcgatc tcgagatcga ggagtacgtc ggctggggac
     1981 ccggcgccag ggtcctgggc tcgacgcaca cggggctgcc gatcgatgtg ccgatcatcc
     2041 agacggacct cgtgatcaag ccagtgcgca tctgcgcgtg ggccgacatc ggcgtgaacg
     2101 ccgtcgtcat gcccggcgtc accgtcggtc gaggtgccat cgtcggcgcc ggggccgtcg
     2161 tcacgcgcga cgtggaaccc tattcgatcg tggcgggcgt gccagcgcga ttcgtgcggc
     2221 gtcggtccgg cggtccagga gagccatgaa gatcgacggt gcgaaaatcg tggtgaccgg
     2281 cggcgccggt ctgatggggt cgacgactgt cgatctcctg ctgactcagc acgaccctcg
     2341 cgccgtggtg atcttcgata acttctcccg cggcacgcag tccaacatcc aggcggcctt
     2401 gagggatccg cgtgtcacgc tcgtcgaggg cgaccttcgc gatcggcagg ccgtggcccg
     2461 ggcgttggcc ggcgccgatg cggtgatcca catggcggcg gtccgcatca tcttgtgcgc
     2521 ggccgatccc cgcctcgcct tcgacgtcat gtgcgggggt gtcttcaacg tcgtcgaagc
     2581 cgcacaggca gccggcgtcc agaagattgt cgcggcgtcg tcggcgtcgg tctacggcct
     2641 ggcggaccag ctcccgacga ccgagaccca ccatccctac aacgatcgca cgtggtacgg
     2701 cgcgtcgaaa atgttcatgg agggcctgct gcgctccttc aacgagatgt acggcctgcc
     2761 atacgtggcc ttgcgctatt tcaacgtgta cgggccgcgc atggacgtcc acggcaagta
     2821 cacggaggtc ctggtccggt ggatggagcg gatggacgcc ggtcagccgc cgctgatcct
     2881 cggagacggc acgcagacga tggatttcgt gtacgtggag gacgtggcgc gcgccaacct
     2941 gctggcgctg gcgtccgacg tgtccgacga tgtgttcaac gtcgccaccg ggattgagac
     3001 gagcctcaac gagctcgcgg cggcgttggc tcgcgccatg ggcgtgcgct gcactccgga
     3061 atacgggccg gagcgaaaga tcaattcagt cccgaggcgc ctgggctcca cagagaaggc
     3121 ggcgcgcgtg ctcggcttca aggcatcagt ggggctcgaa gaggggctgt cgcggctcgt
     3181 gcgctggtgg agggcggagc gggttcgcgt gtaatggaca ccatcccggt agcccgcccg
     3241 cttctcgacg agcgggaagc cgaggctgcc cgccgcgtca tcttgtcagg atgggtcacg
     3301 cagggacccg aggtggcgtc gttcgagcgt gagttcgccg ccgccgtcgg cgctcctcat
     3361 gcgtgcgccg tgtcgagctg cacgacggcg ctccacgtcg cgctgaaggc gtgcggggtg
     3421 gagccgggtg acgaggtcgt caccgtcagc tactcgttca tcgccaccgc caacgccatc
     3481 cgctactgcg gggcgatgcc cgtcttcgtc gacgtgcgcc ccgatggctt caacatcgat
     3541 ccggatgccg tcgagcaggc gatcggcccg cgcacccgtg ccgttctcgt cgtgcaccag
     3601 ttcggtatgc cgtgcgacct tcgcgccatc ctgggcgttg ccggccggca cggggtgccc
     3661 gtgatcgagg acgccgcctg cgccgtgggc agcgaaatcg agtggaacgg gcgatgggag
     3721 cggatcgggc ggccgcacgg cctcgtggcc tgcttctcgt ttcatccccg caagctgctg
     3781 tccaccggcg acggcgggat gctgaccacg gccgacgccg cgctcgaccg ccagttccgg
     3841 ctgctgcgtc atcacggcat gagcgtgtcc gaca
//
LOCUS       DBNGSH010000972         3573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_982947, whole genome shotgun sequence.
ACCESSION   DBNGSH010000972 DBNGSH010000000
VERSION     DBNGSH010000972.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_982947"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="AC16CY_22245"
     CDS             <1..176
                     /locus_tag="AC16CY_22245"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3356589.1"
                     /translation="YFVVEGTGQFVVGGEHRPAGEGMVVWAPAGVSHGVVNRGDRRLV
                     LLVGIAPAPGDHS"
     gene            305..2932
                     /locus_tag="AC16CY_22250"
     CDS             305..2932
                     /locus_tag="AC16CY_22250"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3356590.1"
                     /translation="MALTPGTRLGAYEVTALIGVGGMGQVYRATDTRLKRAVAVKVLP
                     QAVASDADRLARFQREAEVLASLNHPNVAQIYGLEEAGGTSAIVMELVEGPTLADRIA
                     SGPFPLAEALPVAKQCAEALEAAHEQGIVHRDFKPANVKVREDGLVKVLDFGLAKAME
                     PAGAASVGFSQSPTLTTPAMTMAGTIMGTAAYMSPEQARGKPADKRADIWAFGCVLAE
                     MLSGRQVFTGETVSDVMAAILTRDIDWDTVPAVPGKVRELLARCLQKDPLKRLRDIGD
                     ARILIEEALTPSAETPTASPTPAGSNRRRSLAWAFAVVLSFVAGLGAMWIGSDRTDPR
                     PSWSGTKLGGPEVAFGPRVSPKGSLVAFQAMVDGLNQVAVMQPESGDWRVLTEDRSQG
                     YIDCALSWSADERIYYGRWYGVPRGVFSVPAVGGEERLVVENAACPEALADGSLLVIQ
                     INDKGQYQVYRHWPGTDRQPRAYPLQAGVRGFTFLRAFRDGKEAVVWAQSGDQPESDQ
                     CLHLYTLDLTSGSVQDIQTDASLPCEGTEDAGVAVTIGAEDQTILVSTAQGGFARLLE
                     IPRRGGRATQLASTTSKIRGLDTAFNGDVYIDQFTRPMEIISMDPDGGQPRTILTSDT
                     YVNIPAAVMPDGRVLFTSEAGGRRRVLAARPGQNPTPFIQTTEESSGPVALAGHADVA
                     LLIGPSVTRKLAIVSQDGRNVRRLEKADGRSITSLAASADGKTLYYAAEGTIWAIARD
                     GSDPRRIQSGHSVVVDPRGNLIVQLLERDTVRLERVPEAGGQGQALTFPDVRLALMPF
                     TANAVRADGAIIKNISTPDSWLWSPVILFPDTGKVQRVPLPSFLDVHHVGWAPDGKVM
                     VYGLQTHGSLWRLRRSIGD"
     gene            complement(3110..>3573)
                     /locus_tag="AC16CY_22255"
     CDS             complement(3110..>3573)
                     /locus_tag="AC16CY_22255"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the hydrolysis of a monocarboxylic acid
                     amid to form a monocarboxylate and ammonia; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3356591.1"
                     /translation="SLGDRAPVKTLSELRAWNRAHESSGTLKYGQTQLDNADDMDLAA
                     DRARYEADRARDIALTGREGIDAAFTAHKLDALLFPGANGAAIAAKPGYPSVIVPFGT
                     VPNAQMPKEFEARPAPFGVTFTGTACSEGRLIELAYAFEQATKRRSAPPLD"
BASE COUNT          641 a         1119 c         1174 g          639 t
ORIGIN      
        1 tctacttcgt cgtcgagggc acggggcagt tcgtcgtggg cggggagcat cgccccgcgg
       61 gcgaaggcat ggtggtgtgg gcgccggccg gcgtgagcca cggcgtcgtc aaccgcggcg
      121 accggcgcct ggtgctgctg gtcggcattg ctcccgcgcc cggcgatcat tcttgatttg
      181 acgattattg attcttgatt tggttgttga ttggcgattg gcgattgagg attatcaaag
      241 atcacaaatc gtcaacgaat cgataatcaa caatcaagtc aacgatgctc tagagaatcc
      301 ccccgtggcc ctgaccccag gcacccgtct cggcgcgtac gaggtcaccg cgctcatcgg
      361 cgtcggcggg atgggccagg tgtaccgggc taccgacacc agactgaagc gcgcggtcgc
      421 cgtcaaggtt ctccctcaag ccgtcgcatc cgacgccgat cgcttggcgc gattccagcg
      481 cgaagccgag gttctggctt cgctcaatca tccaaacgtc gcgcagatct acggcctcga
      541 agaagccggc ggcaccagcg ccatcgtgat ggaactggtc gaggggccga cgctcgccga
      601 ccggatcgcg agcggtccct ttcctctcgc ggaagcgctg ccggtcgcca agcaatgcgc
      661 cgaggcgctc gaagccgccc acgaacaggg catcgtccac cgcgatttca agcccgccaa
      721 cgtgaaggtg cgcgaggacg gcctggtgaa ggtgctcgac ttcgggctcg cgaaagccat
      781 ggagccggcg ggggccgcct cggtcggctt ctcgcagtca ccgacgttga cgacaccggc
      841 catgaccatg gccggaacga tcatgggcac cgccgcctac atgtcaccgg agcaggcacg
      901 agggaagcct gccgacaagc gtgccgacat ctgggcgttt ggatgcgtgc tggcagagat
      961 gctttcgggt cgtcaggtct tcacaggcga gaccgtctcc gacgtaatgg ccgcgatcct
     1021 gacccgcgac atcgactggg acacggtgcc agccgttcca ggaaaggtgc gcgagctgct
     1081 cgcgcgctgc ttgcagaaag atcctctcaa gcggctccgc gacatcggcg acgcccgaat
     1141 cctgatcgag gaggctttga cgccgtcggc tgaaacgccg acggcgtcac ccacgcccgc
     1201 aggttcgaat cgccgccgtt cactggcatg ggcgtttgcg gtggtcctgt cgttcgtggc
     1261 aggccttggc gcgatgtgga ttggcagcga cagaacggac ccgcgtccaa gctggtcggg
     1321 gacgaagctg ggcgggccgg aagtggcgtt cgggcctcgt gtgtcgccga agggaagtct
     1381 ggtcgccttt caggccatgg tagacggctt gaaccaggtg gccgtgatgc aaccggagtc
     1441 gggcgactgg agggtgctca ccgaggaccg gagccagggc tacatagact gtgcgctgag
     1501 ctggtctgcc gatgagcgca tctactacgg ccgctggtac ggcgtccctc gcggcgtgtt
     1561 cagcgttccc gctgtcggcg gcgaggaacg gctcgtcgtc gagaatgcgg cgtgcccgga
     1621 agccttggcg gacggaagcc tcctcgtcat tcagatcaac gacaaagggc agtatcaggt
     1681 gtaccgccac tggccaggga ctgatcgtca acctcgcgcg taccctctcc aggcgggcgt
     1741 taggggcttt acctttctac gcgcgtttcg agacggcaag gaggcggtag tgtgggcgca
     1801 gtcgggggac cagcccgaat ccgaccagtg cttgcatctg tacacgctgg acctgacgtc
     1861 gggctcggtt caggacatac agaccgacgc gagtctcccc tgtgagggaa ccgaagatgc
     1921 cggcgtggcc gtaacgatcg gtgccgagga ccagacgatt ctggtttcga cagcgcaagg
     1981 cggctttgca cggctactgg agattccccg caggggcggg cgtgctacgc aattggcttc
     2041 gacaacctct aaaatccggg gcctcgacac cgcgttcaac ggcgacgtgt acatcgacca
     2101 gttcacccgg ccgatggaga tcatctcgat ggaccccgac ggcggccagc ctcgcacgat
     2161 cctgacctcg gacacgtatg tcaacattcc ggccgcggtg atgccggacg ggcgcgtgct
     2221 cttcacgagc gaggccggcg ggcgccggcg tgtgctggcc gccaggccgg gccagaaccc
     2281 gacaccgttc atccaaacca cggaagaatc gtctggcccc gtcgcgctgg caggacacgc
     2341 ggacgtggcc ttgctcatcg gcccttccgt aacccgcaag ctcgcaatcg tttcgcagga
     2401 cggacgcaac gtccgccgct tggaaaaggc ggacggccgt tcgatcacgt ccctggccgc
     2461 gtccgcggat ggcaagacgt tgtactacgc agccgaggga accatctggg ccatcgcgcg
     2521 cgacggaagc gatccgcggc gcatccagtc aggccacagc gtggtcgtcg atccacgggg
     2581 gaacctcatc gtccagctac tcgagagaga cacggtgcgg ctggagcgag tgcccgaagc
     2641 cggagggcag ggtcaggcgt tgacttttcc ggacgtccgt ctggcactca tgccttttac
     2701 agcgaatgcg gtccgagccg acggcgccat catcaagaac atcagcactc cagactcctg
     2761 gctctggtcg ccggtcatcc tctttcccga cactggcaag gtacagcgcg ttccgctgcc
     2821 gtccttcctc gatgtccacc acgtgggctg ggcgccggac ggcaaggtga tggtgtacgg
     2881 cctgcagacg cacggctccc tctggcgcct gaggcgatcg attggtgatt aattaagcca
     2941 tggttgattg ttgattcgtc gttgattgtc gattgaggga cttattgtcg gttggaatcc
     3001 ggcgagcatt gcggacttgc gcagcaatcg ccaatcgacc atagatcacc aatcactaat
     3061 caccaatcaa caacgaatcg acaatcaaaa atcgggaaat caacaatgat cagtcgagcg
     3121 ggggagcaga gcgtctcttc gtggcctgct cgaacgcgta ggccagctcg atgagccggc
     3181 cttcgctgca cgccgtgccg gtgaaggtca cgccgaacgg cgcgggcctt gcctcgaact
     3241 ccttgggcat ctgcgcattg ggaaccgtgc cgaaggggac gatcacggat ggatacccgg
     3301 gcttggcggc aatggccgcg ccgttggccc caggaaagag cagcgcatcc agcttgtggg
     3361 ccgtgaacgc ggcatcgatg ccttcgcgcc ccgtcagcgc gatgtctctg gcgcggtcgg
     3421 cctcgtatct ggcccggtcc gccgcgaggt ccatgtcgtc cgcgttgtcc agctgggtct
     3481 gaccgtactt cagcgtgccg ctcgattcgt gcgcgcgatt ccacgcacgc aattccgaga
     3541 gcgtcttgac cggcgcccgg tctccgagcg agg
//
LOCUS       DBNGSH010000973         2024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2342620, whole genome shotgun sequence.
ACCESSION   DBNGSH010000973 DBNGSH010000000
VERSION     DBNGSH010000973.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2342620"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..614
                     /locus_tag="AC16CY_22260"
     CDS             117..614
                     /locus_tag="AC16CY_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356592.1"
                     /translation="MRGPARLLARQALVCAVAVLVAANLHGQTATQPSQDLLLGTWRL
                     DLAKSTYVPGPPPRSETRTYARDGDGVKGTIRRQRADGRREVIEYRADFDHEYPVTGT
                     EAYDTIRLKRIDVRTAEVVLSHAGRVFGRARRVISEDGLTLTIAFRQGDRETFTNNVA
                     VYRKE"
     gene            complement(624..1388)
                     /locus_tag="AC16CY_22265"
     CDS             complement(624..1388)
                     /locus_tag="AC16CY_22265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin family protein"
                     /protein_id="HIR3356593.1"
                     /translation="MTRAVVLAALVALSAPLDAQWHGYPTPGIPRTPDGKPDLSAPAP
                     RAADGRPDLSGIWMASRAVFDLRQALKPGDAVPFTMEGRAVFEERRATNSKDDPSARC
                     LPTGLPVRALLRTPLKIVQAPALTVILYESRTTFRQILTDGRPLPKVVDWPAWQGFSV
                     GAWEGDTFVVQSAGFNGRAWLDQAGYPATDAFRLTERFRRRDFGHIDLEMVIDDRRMY
                     TRPWSVFTELVYQADTELLEFICEENERDAKHMVGK"
     gene            complement(1385..1819)
                     /locus_tag="AC16CY_22270"
     CDS             complement(1385..1819)
                     /locus_tag="AC16CY_22270"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3356594.1"
                     /translation="MRRHVLAASIVTCCVLLAAAGTTLAHHSFAAQFDASKPVTLNGT
                     ITKVEWTNPHTYFYIDVADAKGKVVNWAVEGGAPNVLYREGWRPDSLKIGDRVTISAS
                     RAKDGSNTANATLFMLPDGRCLFAGTSGPGGGATPNCTAGRR"
     gene            1931..>2024
                     /locus_tag="AC16CY_22275"
     CDS             1931..>2024
                     /locus_tag="AC16CY_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HIR3356595.1"
                     /translation="MTLADLRSDTVTRPSAGMRDAIAAAPVGDDQ"
BASE COUNT          326 a          683 c          680 g          335 t
ORIGIN      
        1 gggatctcta cgcagcgctc gagctgccgc atggacgctg gccctggccg gccgtctgag
       61 ttaccctaca ccgcaccgcg gcgcatcgac ggctccggtc tttcagggag gcatccatgc
      121 gtggccccgc tcgtctcctc gcccgtcagg ctctcgtctg tgcagtggcc gtcctcgtcg
      181 cggccaacct tcatggacag acggccaccc aaccgtcgca agaccttctg ctcggcacgt
      241 ggcggctcga cctggccaaa tcgacgtacg tgcctggtcc gcctccgcga tccgagacgc
      301 ggacgtacgc tcgcgacggc gacggcgtca aaggcacgat tcgccggcag cgtgctgacg
      361 gcagaagaga ggtcatcgag taccgggcag acttcgatca cgaatacccc gtaacgggga
      421 ccgaggccta cgacacgatc aggctcaaga gaatcgacgt gcgcacggct gaagtcgtcc
      481 tctcacatgc gggccgcgtc ttcggacggg cccggcgcgt catttccgag gacggcttga
      541 cgctgacgat tgcgttccgg caaggggacc gcgagacgtt cacgaacaac gttgccgtct
      601 accgcaagga gtaggagcgc cggctacttc ccgaccatgt gcttggcatc gcgttcgttc
      661 tcctcgcaaa tgaactccag cagctcggtg tcggcctggt agacgagctc ggtgaaaacc
      721 gaccacggcc tggtgtacat ccgacggtcg tcgatgacca tctcgaggtc gatgtgtccg
      781 aagtcgcgcc tgcggaaccg ctcggtcagc cggaaggcat cggtggcggg gtagcctgcc
      841 tggtcgagcc aggcgcgccc gttgaagccg gccgattgga cgacgaacgt gtcgccttcc
      901 catgcgccga cggaaaaccc ctgccacgcg ggccagtcga cgaccttcgg cagcggccgg
      961 ccgtcggtca ggatctgccg gaacgtcgtg cgcgactcgt agagaatcac cgtcagcgcc
     1021 ggcgcctgca cgatcttgag cggtgtccgg aggagcgcgc ggacgggcag tcccgtcggc
     1081 aaacaccggg cgctcgggtc gtccttcgag ttggtcgccc ttcgctcctc gaatacggcg
     1141 cgcccctcca tcgtgaacgg aactgcgtcg cccggcttga gcgcctgcct gagatcgaac
     1201 acggcgcgcg acgccatcca gatgcccgac aggtccgggc ggccgtcggc cgcgcgtggc
     1261 gccggcgccg acaggtccgg cttgccgtcc ggcgttcgcg gaatgccggg cgtcgggtag
     1321 ccgtgccact gcgcatcgag cggcgccgag agcgcgacga gagcggcgag aacgacggcg
     1381 cgcgtcatcg ccgtcccgcg gtgcagttgg gtgtggcgcc gccgccgggt cccgacgtgc
     1441 ccgcgaagag gcatctgccg tcgggcagca tgaacaacgt cgcgttcgcg gtgttcgatc
     1501 catccttggc tcgcgaggcg ctgatcgtga ccctgtcgcc gatcttcagc gagtccggcc
     1561 gccagccttc gcggtaaagg acattgggtg cgcctccctc gaccgcccag ttcacgacct
     1621 tccctttggc gtctgcgacg tcgatgtaga agtaggtgtg ggggttggtc cactccactt
     1681 tcgtgatcgt tccattcagc gtgacgggct tgctggcgtc gaactgcgcg gcaaacgaat
     1741 gatgagcgag cgtagtcccg gccgccgcga gcagcacaca gcaagtaacg atagaggccg
     1801 cgagcacgtg acgtcgcatg gcgttcctcc gcggaagagc gtctcgagat cccggttgcc
     1861 gagactatgg gggccgggcc ggtcgaagtc aataccggcg gcttgccgcg tcgtcatatg
     1921 atcgggacgc gtgacgctcg ctgacctccg ttccgacacg gtcacgcgcc cgagtgccgg
     1981 catgcgcgac gccatcgccg cggcgccggt cggcgacgac cagt
//
LOCUS       DBNGSH010000974         2788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3191424, whole genome shotgun sequence.
ACCESSION   DBNGSH010000974 DBNGSH010000000
VERSION     DBNGSH010000974.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3191424"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="AC16CY_22280"
     CDS             <1..624
                     /locus_tag="AC16CY_22280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356596.1"
                     /translation="GVNDVPVAMSNFSPGHRITLSAIYTRPFGPTEASFSAYYNGQTG
                     RPYAYRYSNDVNVDGGTTNDLLYIPRDASDVIIANGTFDQLMNFINECEGMTAGAIVA
                     RNSCRGPWTNSMDFGVVFRVPMGRYRGEISFNLQNLINAFDSGNGLVEYATFNGIAPV
                     SAAVDAATGKWRYTLNNVVLAPETNPRFSRDDLRSRWQGQLGFNFRF"
     gene            complement(759..1211)
                     /locus_tag="AC16CY_22285"
     CDS             complement(759..1211)
                     /locus_tag="AC16CY_22285"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3356597.1"
                     /translation="MAAHQHHGAERVVVGKIPQATAEMNITPMIDVLLVLLVIFMVTL
                     PLNQRGLDVNLPAETQQPKQATPDLSQIVLNYTADKKISINNQDVPLGQLGERLRAIF
                     EERTQKTMFIMGAPTLRYGEIVEVIDVAKGAGVEKVGIVTEGMRRAGG"
     gene            complement(1211..1642)
                     /locus_tag="AC16CY_22290"
     CDS             complement(1211..1642)
                     /locus_tag="AC16CY_22290"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3356598.1"
                     /translation="MAMNLGGGGSIKSDINVTPLVDVMLVLLIIMMIVAPLLQQGVEV
                     RLPQAANTTEKPDTQEQTVLAIDANKRLYLNTVPVTAADLRRRIEEALEGKKDRIVII
                     KADQDVEYSAVMDAMDELRASGIEDMGLITDPRQTKVEGGE"
     gene            complement(1709..2482)
                     /locus_tag="AC16CY_22295"
     CDS             complement(1709..2482)
                     /locus_tag="AC16CY_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR3356599.1"
                     /translation="MGVRTIRQFGLVALGVAGFLLISAPAFAQEGGGNIDLVDMFAQM
                     GKVAWGVAIVLFIMSFWSVGIAIERIYTYNQARKQSKMYAPQVAKHLKDNRLKEAIQL
                     SASKDYRYSHLAKVVLAGLQEFQFVQDSGSSLNRDDVLDTVRRSIQRASALTTNDLKK
                     GVNTLATIGSTAPFVGLLGTVVGVINAFVGIAGTGSAGIAGVSAGIAEALVETALGLF
                     VAIPAVWFYNYLTGRLEYFNVEMDNSSSELVDYFIRKTA"
     gene            complement(2537..>2788)
                     /locus_tag="AC16CY_22300"
     CDS             complement(2537..>2788)
                     /locus_tag="AC16CY_22300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3356600.1"
                     /translation="TKTKHVNPQYPTIAQSARVQGVVIIEAIIGPNGKVQDAKVLRSI
                     PLLDQAALEAVKQWEFTPTLLNNNPVPVIMTVTVQFTLN"
BASE COUNT          482 a          908 c          877 g          521 t
ORIGIN      
        1 ggcgtcaacg acgtgccggt cgcgatgtcg aacttctcgc ccgggcaccg catcacgctg
       61 tcggccatct acacccgccc gttcggcccg accgaggcct cgttctccgc gtactacaac
      121 ggccagacgg gccgccccta cgcgtaccgc tacagcaacg atgtgaacgt cgacggggga
      181 acgaccaacg accttctcta catcccgcgc gacgccagcg acgtgatcat cgccaacggc
      241 actttcgatc agctgatgaa cttcatcaac gagtgcgagg gcatgacggc gggagccatc
      301 gtggcgcgca actcctgccg cggcccctgg acgaactcga tggacttcgg cgtcgtgttc
      361 cgggtgccga tggggcgcta ccggggcgag atcagcttca acctgcagaa cctgatcaac
      421 gccttcgaca gcggcaacgg cctggtggag tatgcgacgt tcaacggcat cgcgccggtg
      481 tcggccgccg tcgacgccgc gacgggcaag tggagataca cgctcaacaa cgtggtgctg
      541 gcgcccgaga ccaacccgcg cttctcacgc gacgacctcc gctcccgctg gcagggacag
      601 ctcgggttca acttccggtt ctgagcaggc gggtctaaag acccgcctct acgtgactca
      661 ggccagaaaa gacccgcctc tacgtcggcg cagaagcgac gagtacacaa gaaaggccgc
      721 cttgaggcgg ccttttgttt gagcgggcgg ctgagagtct agcccccggc ccggcgcatg
      781 ccctcggtca cgatgccgac cttctcgacg ccggcgccct tggccacgtc aatcacttcg
      841 acgatctcgc cgtagcgcag cgtcggggcg cccatgatga acatcgtctt ctgagtgcgc
      901 tcttcgaaga tcgcccgcag ccgttcgccg agctggccga gcggcacatc ctggttgttg
      961 atggagatct tcttgtcggc ggtgtagttc agcacgatct ggctcaggtc gggcgtcgcc
     1021 tgcttcggct gctgggtctc ggccggcagg ttgacgtcga ggccgcgctg gttgagcggc
     1081 agcgtgacca tgaagatgac gaggagcacg agcagcacgt cgatcatcgg cgtgatgttc
     1141 atctcggccg tcgcctgcgg gatcttcccg acgacgacgc gctccgcacc gtggtgctgg
     1201 tgggcggcca ttactcgcct ccctcgactt tcgtctgccg cgggtcggtg atgagcccca
     1261 tgtcctcgat cccgctcgcg cgcagttcgt ccatggcgtc catcacggcg ctgtactcga
     1321 cgtcctgatc cgccttgatg atgacgatgc ggtccttctt accctccagg gcctcttcga
     1381 tgcggcgccg caggtccgcg gccgtcaccg gaacggtgtt caagtagagc cgcttgttcg
     1441 cgtcgatggc gagtacggtc tgctcctgcg tatcgggctt ctcggtggtg ttggctgcct
     1501 gcggcagcct gacctccacg ccctgctgga gcaggggcgc gacgatcatc atgatgatga
     1561 gcagcaccag catcacgtcc acgagcggcg taacgttgat gtcggacttg attgagccgc
     1621 cgccgccgag attcattgcc atacgtcagg ctccaccggc gagcacaggc cgggtcgtcg
     1681 aacccggcca gatctcaccc gggacagatc aggccgtctt cctgatgaag tagtcgacca
     1741 gttccgacga ggagttgtcc atctccacgt tgaagtactc gagccgcccc gtgaggtagt
     1801 tgtagaacca cacggcggga atcgcgacga acagcccgag cgccgtctcg acgagcgcct
     1861 cggcgatgcc ggcggacacg ccggcgatac cagccgagcc ggttcccgcg atgccgacga
     1921 aggcgttgat gacgccgacg accgtgccga gcagtccgac gaagggcgcc gtcgagccga
     1981 tcgtggccag ggtgttaacc cccttcttca ggtcgttggt ggtgagggcg gacgcgcgct
     2041 gaatggaacg gcgaaccgtg tcgagaacgt cgtcgcggtt gaggctcgag ccgctgtcct
     2101 gcacgaactg gaactcctgg agtccggcca gcacgacctt cgccagatgg ctgtagcgat
     2161 agtccttcga cgccgagagc tggatggctt ccttcagccg gttgtccttg aggtgcttgg
     2221 ccacctgcgg cgcgtacatc ttcgactgct tgcgcgcctg gttgtaggtg tagatccgct
     2281 caatggcgat gccgaccgac cagaacgaca tgatgaacag cacgatggca acgccccagg
     2341 ccaccttgcc catctgggcg aacatgtcca cgaggtcgat gttgccgcca ccttcctgcg
     2401 cgaacgccgg cgcgctgatc aacaggaatc ccgcgacacc caaggcgacc agtccgaatt
     2461 gacgaatcgt ccgaactccc attactgcat cctccagatc ccgagaattt ggctcgctgt
     2521 gatgacgtgt tcgcgtctag ttgagcgtga actgcaccgt gaccgtcatg atgaccggca
     2581 cgggattgtt gttgagcagc gtcggcgtga actcccactg cttcacggct tcgagcgcgg
     2641 cctgatcgag gagcggaatg gaacgcagga ccttcgcgtc ctggaccttg ccattcggcc
     2701 cgatgatggc ctcgatgatg acgacaccct gcacgcgcgc cgactgcgcg atcgtcgggt
     2761 actgcgggtt gacgtgcttg gtcttggt
//
LOCUS       DBNGSH010000975         3499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2342631, whole genome shotgun sequence.
ACCESSION   DBNGSH010000975 DBNGSH010000000
VERSION     DBNGSH010000975.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2342631"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="AC16CY_22305"
     CDS             complement(<1..305)
                     /locus_tag="AC16CY_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3356601.1"
                     /translation="MDKATFDKGREIRSAVLGKEFVDAAFKAADSFSMPMQELVTEYC
                     WGAVWGREGLDRRTRSLLNLAMISCLNRPHELRLHIAGALRNGVTREEIREVFMQVA"
     gene            complement(398..1285)
                     /locus_tag="AC16CY_22310"
     CDS             complement(398..1285)
                     /locus_tag="AC16CY_22310"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3356602.1"
                     /translation="MAPVLGFVGVGRMGGPMAGRLLDAGHELCVFDTNPAVVAPLSAR
                     GAKSAASADEVASAADIVFMSLPTPPIVQAVCLGERGILKGTRVRTLVDLSTTGPTVA
                     GVIAKVAAERGVAWVDSPVSGGVAGAMKGTLAVMVSCRKDAFPIVDPVLKTFGKTFYV
                     GEKPGLAQIAKLANNLLAAAAIVLSSEAVVMGVKAGLDARTLIDIINAGSGRNSATQD
                     KFPRAILTRTFDFGFATGLSYKDVRLCLDEAEALGVPMIAGAAVRQMLAITNAKFGPD
                     SDFTSIAKVVEEWAGVEIK"
     gene            complement(1295..2758)
                     /locus_tag="AC16CY_22315"
     CDS             complement(1295..2758)
                     /locus_tag="AC16CY_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012707191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR3356603.1"
                     /translation="MYIDGQFVDAASGDSFESDDPFLGGPWALIPRGTAADVDRAVRA
                     AHRAFTSGDWPRLTASRRGALLRRLGDLVTERSKALAEIEVRDNGKLYAEMSMQTAYM
                     AQWYHYYAGLADKIEGAVLPTDKPDTFNYTRYEPLGVVAAIVPWNSPLLLTAWKLAPA
                     LAAGNTVVIKPSEFTSASTLELMKLVEDAGFPPGVVNVVTGFGQDVGAPLVEHPLVAK
                     VAFTGSDATGQRIYEAAARGLKRVTMELGGKSPNIVFDDANIENAVKGVISGIFAATG
                     QTCIAGSRLLVQESIHDAFLEKLVAFARTAKMGNPMDPHTQIGPVTNRPQLEKILRYI
                     DIAKSEGARPVLGGARAERPECGSGWFVEPTIFEGVTNRMRIAQEEVFGPVLAVIPFT
                     DEDEAIAIGNDVVYGLAAGVWTESMRRALKMADRLQAGTVWINTYRAVSYLSPFGGYK
                     RSGIGRESGQEMIKEYLQVKSVWISTVKDVPNPFIQR"
     gene            complement(2801..>3499)
                     /locus_tag="AC16CY_22320"
     CDS             complement(2801..>3499)
                     /locus_tag="AC16CY_22320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356604.1"
                     /translation="MPDWTGLWVWSTKAGFKFDPDTPPGVVTTAKLTPEFQRKLDEEM
                     ARLAKGIEYDPISSCAPPGFPRWLAIPFLREFIVAPDQTWLTSETVNNVRRIYTDGRD
                     HIPEEDRYPLYYGDSIGFWNNQTLTIHTNQLRAGIYQRGNPEYSDRVETVEIWQKVDD
                     RTIEVDVWVYDPPVLLEPWYVRQRYSRISNDDKLVRIRYWDCLENPNNAVYKTKEGGT
                     QFKDFTFTHEDDRK"
BASE COUNT          553 a         1218 c         1075 g          653 t
ORIGIN      
        1 atcgccacct gcatgaagac ctcgcgaatc tcctcgcgcg tgacgccgtt ccgcaacgcg
       61 ccggcaatgt gcagccgcag ttcgtgcggg cgattcaggc agctgatcat cgccaggttg
      121 agcaggctcc gcgtgcgccg atcgagccct tctcttcccc acacggcgcc ccagcagtac
      181 tccgtcacca gttcctgcat cggcatgctg aagctgtcgg ctgccttgaa cgccgcgtcc
      241 acgaactcct tgccaagcac cgcactgcga atctcacgcc ccttgtcgaa cgtcgccttg
      301 tccatgtctt ctccccggat gtgtccgcgg gggctgaagc ccccgctcta cgtcgtactt
      361 ccacttactt gccaacgtcg aactttgaac ttcttactta tttgatctcc acaccggccc
      421 actcctccac caccttcgcg atcgaggtga agtcggagtc gggcccgaac ttcgcgttcg
      481 tgatggccag catctgtcgc accgccgcgc cggcgatcat cggcacgccg agagcctcgg
      541 cctcgtccag gcacaggcgc acgtccttgt acgaaagccc ggtcgcgaag ccgaagtcga
      601 acgtccgcgt caggatggcg cgcgggaact tgtcctgagt ggcactgttc cgcccgctgc
      661 ccgcgttgat gatgtcgatg agcgttctgg cgtcgaggcc ggccttgacg cccatcacca
      721 cggcctccga cgacaggacg atggccgcgg ccgcgagcag gttgttcgcc agcttcgcaa
      781 tctgggcgag ccccggcttt tcgccgacgt agaacgtctt gccgaaggtt ttcagcacgg
      841 gatccacgat cggaaacgcg tccttgcgac aggagaccat caccgccagc gtgcccttca
      901 tcgctccggc gacgccgccg ctcaccggcg aatccaccca ggccacgccc cgctccgccg
      961 caaccttcgc gatcacgccg gcaacggtcg gccccgtcgt cgacagatcg accagcgtcc
     1021 tgacgcgcgt ccccttgagg attccccgct cgcccaggca gaccgcctgc acgatgggcg
     1081 gggtcggcag gctcatgaac acgatgtcgg cggcggaggc cacttcgtcc gccgaggcgg
     1141 cgctcttcgc accgcgggct gacagcggcg cgaccacggc ggggttcgtg tcgaacacgc
     1201 acagctcgtg tcccgcatcg agcagacgcc ccgccatcgg cccgcccatc cgacccacgc
     1261 ccacaaaacc cagcactggt gccatctgtc ctcgttacct ctgtatgaaa ggattgggaa
     1321 catccttgac cgtcgagatc cagacgctct tcacctgcag gtactccttg atcatttcct
     1381 gcccgctctc gcgtccgatc cccgagcgct tgtatccgcc aaacggcgac aggtagctca
     1441 ccgccctgta ggtgttgatc cagaccgtcc cggcctgcag cctgtcggcc atcttcagcg
     1501 cgcggcgcat gctctcggtc cacacgccgg ccgcaagacc atacaccacg tcgttgccga
     1561 tcgcgatcgc ctcgtcctcg tccgtgaacg gaatcacggc gagcaccgga ccgaagactt
     1621 cctcctgcgc gatccgcatg cggttggtca cgccctcgaa gatcgtcggc tcgacgaacc
     1681 agccgcttcc gcattcgggg cgctcggcgc gcgctccgcc cagcacgggc ctggcgcctt
     1741 cgctcttcgc gatgtcgatg tagcggagga tcttctcgag ctgcggcctg ttggtgacgg
     1801 ggccgatctg cgtgtgcgga tccatcgggt tgcccatttt cgcggtccgg gcaaacgcga
     1861 cgagcttctc gaggaacgcg tcgtgaatgc tctcctgtac gagcaggcgc gagcccgcga
     1921 tgcacgtctg gcccgtggcc gcgaagatgc ccgagatgac gcccttcacg gcgttctcga
     1981 tgttggcgtc gtcgaagacg atgttcggcg acttgccgcc gagctccatc gtcacgcgct
     2041 tcaggccccg cgcagccgcc tcgtagatcc gctgccccgt cgcatcggat cccgtgaagg
     2101 cgaccttcgc cacgagggga tgctccacga ggggcgcgcc gacgtcctgg ccgaacccgg
     2161 tgaccacgtt gacgacgccg ggaggaaagc cggcgtcctc gaccagcttc atgagctcca
     2221 gcgtcgatgc cgacgtgaac tccgacggct tgatgacgac cgtgttgccg gccgcgagtg
     2281 ccggcgccag cttccacgcc gtcagcagaa gcggcgagtt ccagggcacg atcgcggcga
     2341 cgacgccgag cggctcgtag cgcgtgtagt tgaacgtgtc gggcttgtcg gtgggcagga
     2401 cggcgccctc gatcttgtcc gcgaggccgg cgtagtagtg gtaccactgc gccatgtacg
     2461 ccgtctgcat gctcatctcg gcgtacagct tgccgttgtc gcggacttcg atctcggcga
     2521 gcgccttcga tcgctcggtg acgaggtcac ccagccggcg aaggagcgcg ccccggcgcg
     2581 aggcggtcag cctcggccag tcgccggacg tgaaggccct gtgcgccgcg cgaactgcgc
     2641 gatcgacgtc tgcagccgtg ccgcgcggaa tgagcgccca tggaccgccc agaaacgggt
     2701 cgtcgctttc gaaactgtcg ccggaggctg catcgacgaa ctggccgtcg atgtacatct
     2761 ggtacctggc gagcgcggcg gtgacgggca cctggcgatc ttatttgcga tcgtcctcgt
     2821 gtgtgaaggt gaagtccttg aactgcgttc cgccttcttt cgtcttgtag acggcattgt
     2881 tcgggttctc gaggcagtcc cagtagcgga tccgcaccag cttgtcgtcg ttgctgatcc
     2941 tcgagtagcg ctgccggacg taccacggct cgaggagcac ggggggatcg tatacccaca
     3001 cgtcgacctc gatcgtgcgg tcgtccacct tctgccagat ctcgacggtc tccacgcgat
     3061 cgctgtactc cgggttccca cgctgataga tgccggcccg cagctggttc gtgtgaatcg
     3121 tgagggtctg gttgttccag aagccgatcg agtcgccgta gtagagcggg taccgatcct
     3181 cctccggaat gtggtcccgc ccgtcggtgt agatgcgccg gacgttgttt accgtctcgc
     3241 tcgtcagcca cgtctggtcg ggcgcgacga tgaactcgcg aaggaacgga atggcgagcc
     3301 agcgcggaaa tccgggcggg gcgcacgagc tgatcgggtc gtactcgatc cctttcgcaa
     3361 ggcgtgccat ctcctcgtcg agcttccgct ggaattccgg cgtcagcttg gccgtggtca
     3421 cgacgcccgg tggcgtatcg ggatcgaact tgaagccggc cttcgtactc cacacccaca
     3481 agcccgtcca gtccggcat
//
LOCUS       DBNGSH010000976         3660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4041155, whole genome shotgun sequence.
ACCESSION   DBNGSH010000976 DBNGSH010000000
VERSION     DBNGSH010000976.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4041155"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="AC16CY_22325"
     CDS             <1..281
                     /locus_tag="AC16CY_22325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356605.1"
                     /translation="GLNRLLDITIPKDKAEGGTYVIPGHGRLSDEADVVEYRDMLTIV
                     RDRVQDLIAQGKSLAEVKAAGPTRDYDGRYGAAAADQLVEALYRELVK"
     gene            complement(286..2913)
                     /locus_tag="AC16CY_22330"
     CDS             complement(286..2913)
                     /locus_tag="AC16CY_22330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3356606.1"
                     /translation="MIERLLRRVRYWLRSRREDAALREELEFHHAMKQQELEREGMDA
                     RNARLAARRDLGNATLARETARAVWIWPWLDSVKQDVSYAVRSLRRQPGFTFVSLIVL
                     GIAIGLNTSFFTVFAGLAIRPMPGVADAGRVVTVSGFNPLVRAVNGFSFPEYEFLASQ
                     SRAIAGLVAHRPMSIRLQTESVGKSTGAYLVTANYFDVLGVRMEHGRRFQPGEDRRGS
                     QAVAILSHELWQTRFGGDAAIVGRQVRVNGLPVTVVGILSRDFGGPEGSASRIWLPFS
                     MQPLLRPNDPFETQMLESAQHCCVNVAGRLATGATRAAAQTEMQLLSGRFRASAGIPE
                     RAIAVEGTQFLRGGRAASGAVAILGVLFVAMTLVLLIACANVGNLLLARATARVREIG
                     VRLSLGAGRGRIVRQLLTEGFVLALIGSALGVLLAALPPIVLRLLSGDSAPFDLKPDG
                     WVLGYGLLLAVVSCVAFALAPALHATSGNVANALKDDSKVLAARFPLRTTLLAVQVAV
                     SVVMLTSAGLLQRGVARASVLDPGFVVDDVSVATIDLPDAYDPARVVSLVSDVTSGLR
                     AAKVERFAFVTNEPLGDNSFMMGMRLQGESEDRMRTIEVVNMSPGYFDTLGIRLVAGR
                     GFDDADAGRDVVIVNESMARLWPDSSALGRSVIIARGGQPIEIVGIVRDSHVASMEAI
                     EPVLFLPIGGSVNVFGDRPPKLLFGTSDPSAAATVAGIVERVDKDARVEITPMKERLD
                     DWLGGLAMAPLAASALGAFALGVATVGMLGVFSYVVRQRTREIGIRMALGARAADVIR
                     VVLASSSKAVAAGLAVGFLGAVAASQVLRGSLYGVSPMDPIAYGAVVLVMAVAALAAS
                     YVPVRRAVQVDPVRALRYD"
     gene            complement(2910..3248)
                     /locus_tag="AC16CY_22335"
     CDS             complement(2910..3248)
                     /locus_tag="AC16CY_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3356607.1"
                     /translation="MGKTDEPPDLLPGTLDLLVLKTLQRGVMHGYGIAQHLRTASDEV
                     VQVGESSLYPALQRLLLNGYVKAEWGASENNRRARYYTLTAAGRKQLASERQEFDRLI
                     AAIQKVLATS"
     gene            complement(3352..>3660)
                     /locus_tag="AC16CY_22340"
     CDS             complement(3352..>3660)
                     /locus_tag="AC16CY_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3356608.1"
                     /translation="ALVETYAVLTRLPPPHRLTAKDAWALIEGNFVTGALLEVLDGGG
                     YVDLLRRAASQDVAGGRTYDAVIGACAREAGATVLLTFNRRHFDPAPPGLQVLEPALA
                     "
BASE COUNT          645 a         1282 c         1226 g          507 t
ORIGIN      
        1 acggcctgaa tcggctgctc gacatcacca tccccaagga caaggccgag ggcggcacct
       61 acgtcattcc ggggcacggg cggctgtccg acgaagccga cgtcgtcgag taccgcgaca
      121 tgctgaccat cgtccgcgat cgcgtccagg acctgatcgc gcaggggaag tcgctcgccg
      181 aggtgaaggc cgccggcccg acgcgtgatt acgacggccg ctacggggcc gccgccgcgg
      241 atcagctcgt cgaagccctg tatcgggaat tggtgaagta accgcttagt cgtatcgaag
      301 ggcccgaacg ggatcgacct gcacggcccg gcgaacggga acgtagctgg ccgcgagggc
      361 cgcgactgcc atcacgagga ccacggcgcc gtacgcgatc gggtccatgg ggctcacccc
      421 gtacagcgag ccgcggagga cctgcgacgc ggcgaccgcg ccgagaaacc cgacggcaag
      481 acccgccgcc acggccttcg agctcgacgc gaggactacc cggatcacgt ctgcggcgcg
      541 cgcgccgagc gccatgcgga tgccgatctc ccgggtccgc tgccggacga cgtaggagaa
      601 cacgccgagc atgccgacgg tggcgacgcc gagcgcgaac gcgccgagcg ccgacgccgc
      661 gagcggcgcc atcgcaagtc cgcccagcca gtcgtcgagc ctttccttca tcggagtaat
      721 ttcgacgcgc gcgtccttgt ccacacgctc gacgatcccg gcaaccgtcg cggcggctga
      781 cggatccgag gttccaaaga gcagcttcgg cggccgatcc ccgaacacgt tgacactgcc
      841 tccgatcggc aggaacagca ccggctcgat ggcctccatg ctggcgacgt gagagtcccg
      901 gacgatcccc acgatctcga tgggctggcc gccccgggcg atgatgacgg accggcccag
      961 ggcactgctg tccggccaga gacgggccat cgactcgttg acaatgacca cgtctctgcc
     1021 ggcgtcggcg tcgtcgaagc ctcgccccgc aacgaggcga atcccgagcg tgtcgaaata
     1081 ccccggcgac atgttgacga cctcgatggt cctcatccga tcctcggact caccctgaag
     1141 gcgcatgccc atcatgaagc tgttgtcgcc aagcggttcg ttcgtgacga acgcgaaacg
     1201 ttccactttc gcggcccgca gccccgacgt gacgtctgac accagcgaca ccactcgcgc
     1261 cggatcgtat gcgtccggaa ggtcgatggt ggccaccgac acgtcgtcaa cgacaaatcc
     1321 tggatccagc acgctggccc gcgcgacgcc gcgctggaga aggccggcgc tggtgagcat
     1381 cacgaccgac acggcgacct gcacggcgag gagcgtcgtg cggagcggaa accgcgccgc
     1441 gagaaccttc gaatcgtcct tgagcgcgtt cgccacattg ccgctcgtcg catgcagcgc
     1501 cggcgccagc gcgaaggcca cgcaggacac gacggcgagc agcagaccgt agccgagcac
     1561 ccatccgtcc ggtttcaagt cgaagggggc cgagtcgcca gacaggagtc gaagcacgat
     1621 tggcggcagc gcggcaagga gcacgcccag agcgctgccg atcaacgcca ggacaaaacc
     1681 ctcggtcagc agctgccgga cgatgcgccc gcggccggcc ccgagcgaca ggcgcacccc
     1741 gatctcccgc acgcgcgcgg tcgcgcgggc gagcagcagg ttgccgacgt tcgcgcacgc
     1801 gatgaggagc acgagcgtca tcgccacgaa cagcacgccc aggatggcga cggcgccgct
     1861 ggcggcgcgc ccgccgcgga gaaactgcgt gccctcgaca gcgatggccc gctccggaat
     1921 gccggcgctg gcgcgaaacc gcccgctcag gagctgcatc tccgtttgcg ccgccgcgcg
     1981 cgtcgcgccc gtcgcaagcc ggccggccac gttcacgcag cagtgttggg cgctctcgag
     2041 catctgcgtt tcgaacggat cgttggggcg aagcagcggc tgcatcgaga acggaagcca
     2101 gatccggctg gccgatcctt cgggcccgcc gaaatcgcgc gacaggatcc cgaccaccgt
     2161 cacgggcaat ccgttgacac ggacctgcct cccgacaatg gccgcgtcgc cgccaaagcg
     2221 cgtctgccac agctcgtgac tcaggatggc gacagcctgc gacccacggc ggtcctcgcc
     2281 gggttggaag cgccggccgt gttccatccg gacgccgagg acgtcgaagt agttcgcggt
     2341 cacgagatag gcgcccgtgg actttccgac gctctcggtc tggagacgga tcgacattgg
     2401 ccggtgggcc acgaggccgg caatcgctcg cgactgcgac gccaggaatt cgtattcggg
     2461 aaacgaaaat ccgttcacgg cacgcacgag tggattgaag ccagacacgg tgacgacccg
     2521 gccggcgtcg gccacgcccg gcatgggacg gatggccagg cccgcgaaca ccgtgaaaaa
     2581 actcgtgttg agtccgatcg cgatcccgag cacgatgagc gacacgaacg tgaagccggg
     2641 ctgacgccgg aggcttcgaa cggcgtagct cacgtcctgc ttcacgctgt cgagccaggg
     2701 ccagatccag acggcgcgag ccgtctcgcg cgccagcgtc gcgttcccga ggtcgcgacg
     2761 cgcggccagt cgagcgttgc gggcgtccat cccctcccgc tcgagctcct gctgcttcat
     2821 cgcgtggtgg aactcgagct cctcgcgcag cgccgcgtcc tcgcggcggg atcgaagcca
     2881 gtaccgaacc cgccgcagca gccgctcgat catgacgtcg ccagcacttt ctgaatggcc
     2941 gcaatcaggc ggtcgaactc ctgccgctcg gaggcgagct gcttccgccc cgccgccgtc
     3001 agcgtgtagt agcgggcccg ccggttgttc tcggaggcgc cccactccgc cttgacgtat
     3061 ccgttgagga gcaggcgctg cagggcggga tagagcgagc tctcgcccac ctgcaccacc
     3121 tcgtcggacg cggttctcag gtgctgcgcg atgccgtagc cgtgcatcac gccgcgctgc
     3181 agcgtcttca gcaccagcag gtccagggtg cccggcagca ggtcaggcgg ctcgtcggtt
     3241 ttgcccatgc gctcccatcg ttaaacgatg ggagcatcgt cgatcgcgcg ggggatgact
     3301 gtcaagcccc atcgtcagtc gatggggcgc ggcggacctc gtgtcgagac gtcaggcgag
     3361 ggccggctcg aggacctgga gcccgggagg cgccgggtcg aagtggcgcc ggttgaacgt
     3421 cagcaggacc gtcgcaccag cttcgcgggc acacgcaccg atgaccgcgt cgtaggttct
     3481 ccccccggcg acgtcctgac tggcggcgcg gcgcaggaga tcgacgtacc cgcctccgtc
     3541 gagtacctcg agcagagctc cggtcacaaa gttgccctcg atgagcgccc aggcgtcttt
     3601 ggccgtcaac cgatgcggcg gcggcagacg cgtgagaacg gcgtaggtct cgacgagggc
//
LOCUS       DBNGSH010000977         1599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1154917, whole genome shotgun sequence.
ACCESSION   DBNGSH010000977 DBNGSH010000000
VERSION     DBNGSH010000977.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1154917"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="AC16CY_22345"
     CDS             <1..549
                     /locus_tag="AC16CY_22345"
                     /inference="COORDINATES: protein motif:HMM:NF016199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HIR3356609.1"
                     /translation="IVTTLGADGSVNCAPMGVEWGEEVIVLKPFLETATYRNVLATEA
                     AVVNLVDDARVFARAAISNPDYPTIPAEVVRGVVLSDCCSWRELTVRSIDSTPPRSRI
                     EMSVVHRGVRREFIGFNRARHAVLEAAIYATRLHLLERAFLESEMARLQVIVDKTAGP
                     DEREAMALLTEHMRKTWATGRV"
     gene            617..>1599
                     /locus_tag="AC16CY_22350"
     CDS             617..>1599
                     /locus_tag="AC16CY_22350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3356610.1"
                     /translation="MWLLGALVVILAAGACYQAVFTARQRRLFAPPGTLIDVGGHRLH
                     AVCAGEGSPPVLLESGIAASSLSWAAVRPEIAAFTRVCAYDRAGLAWSDAPSSPRTFD
                     RLLDELGIVMRDVGFREPAVLVGHSFGSLLVRAYGARHPERVAGLVLVDPPTEWIVMT
                     PARARMLRGGRSLSRIGALLAQVGVVRGCLALLSGGAPGVPRRFVRIFGPTAARTLER
                     LVGEVRKLPPEVHPVVQAHWCQPKCFHSMADHLLMFEREAAQMTALAPPPHLPVVVVS
                     SAGHSPEQLAAHQRLAGSSARGRHVIARRSAHWVQFDEPGLIVGIVREMVE"
BASE COUNT          213 a          545 c          560 g          281 t
ORIGIN      
        1 atcgtgacga cgctcggtgc ggacgggagc gtgaattgcg ctccaatggg cgtcgaatgg
       61 ggcgaggagg tcattgtcct caagccgttt ctcgaaaccg ccacgtaccg gaacgtgctg
      121 gcgacggagg cggctgtcgt caatctcgtt gacgacgcgc gcgtgttcgc gcgggcggcg
      181 atctccaatc ccgattaccc cacgatcccg gccgaggtgg tgcgcggagt cgtgctgtcc
      241 gactgctgct cgtggcgcga gctgaccgtc cgctcgatcg acagcacgcc gccccgctcc
      301 cggatcgaga tgtcggtcgt gcaccgcggc gtgcgtcgcg agttcatcgg tttcaaccgc
      361 gcccgccacg ccgtgctcga agcggcgatc tacgcgacca ggctgcacct gctggagcgc
      421 gcgttcctcg aaagcgagat ggcgcgtctc caggtcatcg tggacaagac cgccgggccc
      481 gacgagcggg aggcgatggc gctgttgacc gagcacatgc gcaagacgtg ggccaccggg
      541 cgggtctgaa gacccacccc tacgtaccag tagcggcgcg tcttcagacg cgccggactt
      601 ctggcacgat cgcgaggtgt ggctccttgg tgccctggtc gtgatcctgg ctgccggcgc
      661 gtgctatcaa gcggtcttca cggcccgcca gcgccgtctc tttgctccgc cagggacgtt
      721 gattgacgtt ggtggacatc gtctccacgc cgtctgcgcc ggggagggaa gcccgcccgt
      781 cctgctggag tcggggatcg cggcgtcgtc gctgagctgg gcggccgtgc ggcccgagat
      841 cgcggccttt acgcgcgtct gcgcgtacga ccgcgccggc ctcgcgtgga gcgacgcgcc
      901 gtcgtcgccg cggaccttcg atcggcttct cgacgagctg ggcatcgtga tgcgcgacgt
      961 cggcttcaga gaacccgctg tcctcgtggg ccactcgttc ggcagccttc tcgtccgggc
     1021 gtacggcgca cgccatcccg agcgggtcgc cggactggtg ctcgtcgatc cgcccaccga
     1081 atggatcgtg atgaccccgg cgcgcgcccg gatgcttcgc ggtggacgat cgctctccag
     1141 gattggcgcg ctcctggcgc aggtcggcgt cgtccgtgga tgcctcgcgc tcctctcggg
     1201 tggcgctcca ggcgttccac ggcgctttgt caggatcttc ggtccgacgg cggcacgaac
     1261 cctcgagcgg ctcgtgggcg aagtgcggaa gcttccgccc gaggtccacc ctgtcgtgca
     1321 ggcgcactgg tgtcagccga agtgctttca ctcgatggcc gaccatctgc tgatgttcga
     1381 gcgggaggcc gcgcagatga cggcgctcgc gccgccgccg caccttcccg tcgtcgtcgt
     1441 gtcgagcgcc ggccattcgc cggagcagct cgccgcgcac cagcggttgg ccgggagctc
     1501 cgcgcgcggg cgccacgtga tcgcacgaag gagcgcccac tgggttcaat tcgacgagcc
     1561 cgggctcatc gtcggaatcg tccgtgagat ggtcgagca
//
LOCUS       DBNGSH010000978         3717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1494408, whole genome shotgun sequence.
ACCESSION   DBNGSH010000978 DBNGSH010000000
VERSION     DBNGSH010000978.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1494408"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="AC16CY_22355"
     CDS             <1..884
                     /locus_tag="AC16CY_22355"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356611.1"
                     /translation="RHARGLLRRTGAGGLRRSIESHVGVMRAIAVIAGGVFRDSIRDR
                     IAYGLVFFAVLLIASSFLLARLTAGQDVKIIKDLGLAASSGIGVLIAVFFGIGLVTKE
                     VERRSIYSLLSKPVSRTEFVVGKYVGLVLTLLINLSVMVAAFYAVLAVLNYLAIPDLR
                     ASWDAPALDPRMLVAFALIGVELMLVTATALFFSTFSSPFLSVALTFAIYIIGHFGED
                     LKHLDNIVQSPALAQVARGVYYVMPNLAALDVKSDVVHGVAVSAQHVLAASAAAGLYI
                     ALILVAATVIFSRRDLK"
     gene            895..1785
                     /locus_tag="AC16CY_22360"
     CDS             895..1785
                     /locus_tag="AC16CY_22360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356612.1"
                     /translation="MRGPLISPWQWIALALLLPAAVGAQVAFEDVYAPRETRPETLWV
                     RSPGLMRRLSVGFAAVMADVYWIRAVQYYGDTKLSLSTNRNYDLLYPLLDTTTTLDPR
                     FNIAYRFGAILLSEGYPNGPGEIDGAIALLQKGIRAMPEKWQYYHDAGFVEYWWRDHK
                     AASEWFLKASKVPGAPPFLPTIAARALAVGGDRATARALWQGLATAEHEWLRQSARRA
                     LMRLDAEDEIEALQRIVDAFHGDTGRYPAGWIELAQLGRVRRIPLDPSGVPYALDPVS
                     GAVSVTPDSALYPLRPVRKP"
     gene            1800..2555
                     /locus_tag="AC16CY_22365"
     CDS             1800..2555
                     /locus_tag="AC16CY_22365"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR3356613.1"
                     /translation="MTALIALLGLCIGSFLNVCIFRLPRGESLAWPGSRCPSCGRTLS
                     WFENVPIVSWAVLRGRCRTCRTPISWLYPLVELLTAVTLVGAYELYGLTAFGGVRALF
                     ACSLIVLFVTDLQHKILPNVITLPGIVIGFLSSLFLPPGWFDSLIGIVVGGGTLFVIS
                     EAYYRLRGQEGMGMGDVKLLGMIGAFLGWKLVLLTLVFASFAGSLVGVAFIASGRGSM
                     QYALPFGTFLAIGAFAAAIWGTPVVDWYFGFYG"
     gene            2552..>3717
                     /locus_tag="AC16CY_22370"
     CDS             2552..>3717
                     /locus_tag="AC16CY_22370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3356614.1"
                     /translation="MNPQAYALLGLTALVAMIVGAAVFAVLRFAAAARDNRHLFRDNA
                     AQSPLLGAALQEAILKLRAQERATAVRAEASERLNTQIVTSLTAGLLVVDRDGNVQIL
                     NPAGQRMLGIAEGAWSHVSALPPAAAPLARAIEECLSTGQPIVRRALEMPNAGPVTHV
                     GVTVSPLGPVQDSTGGVICLFSDLTTVIQLEDQLRLKETLARLGELTAGIAHEFRNGL
                     ATIHGYGKLLDPDALPPQFRPYVEGIRQETEALGQVVTNFLNFARPTQLSLATHDLGA
                     IVDRAADDLKSEARQLKGDIVVAGDFGSVEADDVLLRQAFMNLLRNAVEACQGHGVAP
                     AVRVQATLDRTQKTVRIDVHDNGPGVSGDARERVFRPFFTTKKDGTGLGLALVQ"
BASE COUNT          533 a         1252 c         1229 g          703 t
ORIGIN      
        1 cgcgccacgc tcgaggactt cttcgtcgaa caggtgcggg cggcctccgg cgatcgatcg
       61 aaagccacgt tggcgtcatg agagcgattg cggtcatcgc cgggggggtg ttccgcgact
      121 cgattcgtga tcgcatcgcg tacggactgg tgttcttcgc cgtgctgttg atcgcctcgt
      181 cgttcctcct ggccaggctc accgccggac aggacgtcaa gatcatcaag gacctcgggc
      241 tggccgcctc gtcgggaatc ggcgtgctca ttgccgtgtt cttcggcatc ggcctggtca
      301 ccaaggaagt ggagcggcgc tcgatctact ccctgctctc gaaaccggtc agtcgcaccg
      361 agttcgtcgt cggcaaatac gtcgggctcg tgctgacgct cctcatcaac ctgtcggtca
      421 tggtcgccgc cttctacgca gtgctcgcgg tcctgaacta ccttgccata ccggatctca
      481 gggcgtcgtg ggatgcgccg gcgctggatc cgcgcatgct cgtcgcgttt gcgctcatcg
      541 gcgtcgagct gatgctcgtc accgcgacgg cgctcttctt ctcgacgttt tcgagcccgt
      601 tcctgtcggt cgcactcacc ttcgccatct acatcatcgg gcacttcggc gaggacctga
      661 aacacctcga caacatcgtg cagtcgccgg cgcttgcgca ggtcgcgcga ggtgtctact
      721 acgtgatgcc gaacctggcc gcgctcgacg tcaagtcgga cgtggttcac ggcgtggccg
      781 tcagcgcgca gcacgtgctt gccgcgagcg cggcagccgg tctgtacatc gcgttgatcc
      841 tcgtcgcggc gacggtcatc ttctcgcgcc gcgacctcaa gtagggctcg atacatgcgc
      901 ggcccgctca tctcgccctg gcaatggatc gcgttggcgc tgctgctgcc cgccgccgtc
      961 ggtgcccagg tggccttcga ggacgtctac gccccccgtg agaccaggcc ggagacgctg
     1021 tgggtgcgct ctccagggct gatgcggcgc ctgtcggtcg gcttcgccgc ggtcatggcc
     1081 gacgtgtact ggattcgtgc cgttcagtac tacggcgaca cgaagctctc cttgtcgacg
     1141 aacaggaact acgacctgct ctatccattg ctcgacacga cgacgacgct cgatcctcgc
     1201 ttcaacatcg cgtatcgctt cggcgcgatc ctgctgtcgg agggatatcc gaacgggcca
     1261 ggcgagatcg atggagcgat cgcgctgctg caaaagggga ttcgggccat gccggagaag
     1321 tggcagtact accacgacgc ggggttcgtg gagtactggt ggcgggacca caaggcggcg
     1381 tcggagtggt tcctcaaggc gtcgaaggtg cccggcgcgc cgcccttcct gccgacgatc
     1441 gccgcgcggg cgctcgccgt tggaggcgat cgcgccacgg cgcgcgcgct gtggcagggg
     1501 cttgccacgg cggagcacga atggttgcgg cagtctgccc gccgcgcgct catgcgcctg
     1561 gacgccgagg atgagatcga ggcgcttcaa cggatcgtcg acgcgtttca cggcgacaca
     1621 ggccgctacc cggccggatg gatcgagctt gcgcagctgg gccgggtcag gagaataccc
     1681 cttgatccat cgggtgtccc gtacgcgctc gatcccgtct cgggagcggt cagcgtgact
     1741 cccgattctg cgctctatcc gctgcggccg gtccggaagc cgtgattcct gagcccgcct
     1801 tgaccgcgct catcgcgctg ctcgggctct gcatcggcag cttcctcaac gtgtgcatct
     1861 tccgcctgcc gcgcggcgag tcgctcgcct ggccgggctc acggtgcccg tcgtgcggcc
     1921 gcacgctgtc gtggttcgag aacgtcccga tcgtcagctg ggccgtcctc aggggccgct
     1981 gccgtacctg ccggacgccg atctcgtggc tctatcccct cgtggagttg ctgaccgccg
     2041 tgacgctggt cggcgcttac gagctctacg gactgacggc gttcggcggc gtcagagcgc
     2101 tcttcgcctg ctcgctgatc gtgctcttcg tgaccgattt gcagcacaag atcctgccca
     2161 acgtcatcac gctgccaggc atcgtcatcg gtttcctcag cagcctgttc ctgccgcccg
     2221 gctggttcga ttcgctgatc gggatcgtcg tgggcggcgg cacgctgttc gtgatctcgg
     2281 aggcctacta tcgtctccgc ggacaggaag ggatggggat gggcgatgtg aagctgctcg
     2341 gcatgatcgg cgcgtttctc ggatggaagc tcgtactgct gaccctcgtg ttcgcgtcct
     2401 tcgccgggtc gctggtcggc gtggccttca tcgcgtccgg gcgggggagc atgcagtacg
     2461 cgctgccgtt cggcaccttc ctcgccatcg gcgcgttcgc cgcggccatc tggggcactc
     2521 ccgtcgtcga ctggtatttc gggttctacg gatgaatcct caggcatacg cgctgctcgg
     2581 attgacggcg ctcgtcgcga tgatcgtcgg cgccgccgtg ttcgccgtcc tccgcttcgc
     2641 tgcggcggcg cgggacaacc gccacctctt tcgcgacaac gcagcgcagt cgccgctgct
     2701 cggcgccgcg ctccaggagg cgatcctgaa gctgcgggca caggagcgcg ccacggccgt
     2761 gcgagcggag gcttccgagc gcctcaatac ccagatcgtc accagcctga cggccggcct
     2821 gctcgtcgtg gatcgcgacg gcaacgtgca gattctcaat ccggcggggc agcggatgct
     2881 cgggatcgcg gaaggcgcgt ggtcccacgt gtcagcgctg ccgccggcag cggcgcctct
     2941 ggctcgggcg atcgaggagt gtctctcgac tggtcaaccc atcgtgcggc gtgcgctcga
     3001 gatgccgaac gcggggccgg tgacgcacgt gggggtcacc gtgtcgcccc tcggtcccgt
     3061 gcaggacagc acgggcggcg tgatctgtct gttctcggac ctgacgaccg taattcagct
     3121 cgaggatcaa cttcggctga aggaaacgct ggccaggctc ggcgagctca ccgccggcat
     3181 cgcccacgag ttccgcaacg gcctggcgac gatccacggc tacggcaagc tgctcgatcc
     3241 cgacgcgctt ccgccacagt tcaggccgta cgtggaaggc atccgccagg aaacggaagc
     3301 gctcgggcaa gtggtcacca acttcctcaa cttcgcccgg ccgacgcagc tctcgctcgc
     3361 cactcacgac ctcggcgcga tcgtcgatcg cgccgccgac gatctgaagt cggaggctcg
     3421 ccagctgaag ggcgatatcg ttgtcgcagg cgatttcgga tcggtcgagg cagacgacgt
     3481 gctgctgcgg caggcgttca tgaatctcct tcggaacgcc gtggaggcgt gccagggcca
     3541 cggcgtcgcg ccggccgtgc gcgtacaggc cacgctcgac cggacacaga agaccgtccg
     3601 catcgacgtc cacgacaatg gccccggcgt gtcgggcgac gcgcgcgagc gcgtcttccg
     3661 tcccttcttc acgaccaaga aagacgggac gggcctcggc ctcgcgctgg tccagaa
//
LOCUS       DBNGSH010000979         3329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_136769, whole genome shotgun sequence.
ACCESSION   DBNGSH010000979 DBNGSH010000000
VERSION     DBNGSH010000979.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_136769"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..929)
                     /locus_tag="AC16CY_22375"
     CDS             complement(153..929)
                     /locus_tag="AC16CY_22375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3356615.1"
                     /translation="MSVAVPDLVTIGLLVVLEGLLSADNALVMAIMVLGLPKRDHMKA
                     LRYGLLGGFAFRIAATMLAVYLIQLAWVKVLGGLYLMYLTYTHFWGQEEGSDRRLAPR
                     ARPWLGLPAFWATVVKVELINLAFSIDSILVAVAMSPKLWVIITGGILGIVAMRLVAG
                     HLITLVQRYPALVDGAFIIIAWVGLKLCLEYLHEAHIIGLEIPRWLSLGLIVVIFVVA
                     LVYARAQGAVEGVDPLTEEAGDILAHDSEVVASIDGDVTK"
     gene            1004..1405
                     /locus_tag="AC16CY_22380"
     CDS             1004..1405
                     /locus_tag="AC16CY_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3356616.1"
                     /translation="MSTLDVYQGFGATALGVEASVLDQDLFFISMAADRLGMHPQTLR
                     KYERLGLVRPSRTLGSMRLYTPSELERLRLIKQLVERHRINLAGIQRLLAVAEVMQRI
                     HPLMDETALSTGAGRRQLLNEIKRLNDIIGL"
     gene            1407..2396
                     /locus_tag="AC16CY_22385"
     CDS             1407..2396
                     /locus_tag="AC16CY_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR3356617.1"
                     /translation="MEFKDYYRTLGVTKASGDKEIKQAFRKLARKYHPDVNPGDKAAE
                     AKFKEINEAYEVLGDPSSRKKYDELGANWRMYEQQAQSGGHPFAGAWNVGGSGSRGGF
                     RTMSPEEMEDLFGDSNPFSDFFTTFFGGGSGEAAGHGAGRARRRQGSDVENEIELALE
                     DAYYGTTRRLSLRHDGHARTVDVRIPPGVTDGSRVRVAGEGGQGVGGATSGNLYLRVK
                     LAEHAVFERKGRDLYVKTPVSVTTAVLGGEAQVPTIAGKPARLRIPPLTQNGQVFRLK
                     GYGMLAVGKPQDTGDLYARVDVQLPSSLTEKEREHYEALKNLGGDDKNSHSAV"
     gene            2408..>3329
                     /locus_tag="AC16CY_22390"
     CDS             2408..>3329
                     /locus_tag="AC16CY_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR3356618.1"
                     /translation="MNLNSLTEKAQEAVLGAQNLATEQNHSEVTPEHLFVTLVEQPGG
                     IVPSLMRKLSLDPSRVSAEARDLLKAMPQAYGADLRLSPRMKLILDSAQAEAKRLQDQ
                     FVSTEHLLLAIATESGRSPAAQMLQRLGATKDALYKALTDVRGNQRVTSQNPESTYEA
                     LVRYGRDLTELARDGKLDPVIGRDEEVRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVRGDVPEGLKNKKIFALDMGALVAGAKYRGEFEERLKAVLKEIAESEGQIVLF
                     IDELHTVVGAGAAEGAMDASQMLKPMLARGE"
BASE COUNT          662 a         1058 c         1122 g          487 t
ORIGIN      
        1 agaccatcgc caacatctac gactacacct acgcgctgga gttgctgaag atcggcgagc
       61 cggtcgcggt cgtctacatg cgcggcgcgg agaagcgcga gaccacgctc acgccagcgg
      121 caagacggta ggtcccgctg gtttgtagcg gcctacttcg tcacatcccc gtcgatgctc
      181 gccacgactt cgctgtcgtg ggccagaata tcccccgctt cttccgtcaa cggatcgacg
      241 ccctcgactg ccccctgcgc tctggcgtag acgagcgcca cgacgaagat gacgacgatg
      301 agaccgagcg agagccaccg tggaatctcc agcccgatga tgtgcgcctc gtggaggtac
      361 tcgagacaca gcttgagtcc cacccaggcg atgatgatga acgcgccgtc cacgagagcc
      421 ggataccgct gcaccagcgt aatgaggtga ccggcgacga gacgcatcgc cacgatgccg
      481 agaatgccgc cggtgatgat gacccacagc ttcggcgaca ttgcgacggc cacgaggatc
      541 gagtcgatcg agaacgcgag attgatcagc tcgactttca cgacggtggc ccagaaggca
      601 ggcaggccaa gccagggtct ggcccggggc gccagacggc ggtcgctgcc ctcctcctgg
      661 ccccagaagt gtgtgtaggt caggtacatc aggtagagcc cgccgaggac cttcacccat
      721 gccaactgga tgagatacac ggcgagcatc gtcgcggcga tccggaaggc gaagccgccg
      781 agcaaaccgt acctcagcgc cttcatgtga tcgcgcttgg gcaggcccag gaccatgatg
      841 gccatgacca gggcgttgtc agcgctgagg agcccttcga ggacgacgag aagaccgatg
      901 gtgacgaggt ccggaaccgc gacgctcaag gccttcgatc ctaccctgcc ccggggaaag
      961 cgccatcaga ccgagccggc caatattgac acgagtcgta tcaatgtcta cactcgatgt
     1021 gtatcagggt ttcggcgcaa cggctctggg cgtggaggca tcagtcttgg accaggacct
     1081 gttcttcatc agcatggcgg cggaccggct ggggatgcat ccccagacgc tgcggaaata
     1141 tgagcgcctc gggctggttc gtccgagtcg cacgctcggc agcatgcgtc tctacacgcc
     1201 gagcgagctc gaacggctgc gtctcatcaa gcagttggtc gagcggcacc ggatcaacct
     1261 ggccggcatc cagcgcctgc tggccgtggc agaagtgatg cagaggattc acccgctcat
     1321 ggacgagacc gcgttgagca ccggcgccgg ccggcgccag ttgctgaacg agatcaaacg
     1381 cctcaacgac atcatcgggt tgtagcgtgg agttcaagga ctactacagg acgctcgggg
     1441 tgaccaaggc ctccggggac aaggagatca agcaggcgtt ccggaagctc gcccgcaagt
     1501 accaccccga cgtcaacccg ggtgacaagg ccgcggaggc gaagttcaag gagatcaacg
     1561 aggcgtacga agtcctcggc gatccgtcga gccgcaagaa gtacgacgaa ctcggcgcga
     1621 actggcggat gtacgagcag caggcccaga gcggcggcca tccgttcgcc ggggcatgga
     1681 acgtgggcgg cagcggatcg cgcggcggct tccgcacgat gtcgccggag gaaatggagg
     1741 acctgttcgg cgattccaac ccgttctccg actttttcac cacgttcttc ggcggcggaa
     1801 gcggcgaggc tgccggccac ggcgcgggcc gggcccgccg gcgccaggga agcgacgtgg
     1861 agaacgaaat cgagctggcc ctcgaggacg cgtattacgg aaccacgcgg cggttgtcgt
     1921 tgcggcacga cgggcacgcc cgaaccgtcg atgtgaggat ccctcccggc gtcaccgacg
     1981 gatcgcgggt gcgcgtcgcc ggggagggcg gacagggcgt cggcggagcc acgtccggca
     2041 acctgtacct gagagtcaag ctcgcggaac acgccgtgtt cgagcgcaag ggacgggatc
     2101 tctacgtcaa gactccggtc agcgtcacga cggcggtcct cgggggcgaa gcgcaggtgc
     2161 cgaccattgc gggcaagccc gcgcggttgc gaattccgcc gctcacgcag aacgggcagg
     2221 tcttccggct gaagggatac gggatgctcg ccgtcggcaa accgcaggac accggcgatc
     2281 tctacgcccg ggtggacgtg cagctgccgt cgtcgctgac ggagaaggaa cgcgaacact
     2341 acgaggcgct caagaacctc ggcggcgacg acaagaactc acacagcgcg gtgtaaggaa
     2401 cagtccgatg aatctcaact cgctcaccga aaaagcccag gaggccgtgc tcggggcaca
     2461 gaacctcgcc acggaacaga accactcgga agtcacgccc gagcacctgt tcgtgacgct
     2521 cgtcgaacag cccgggggca tcgttccgtc gctgatgcgg aagctgtctc tcgatccctc
     2581 tcgtgtgagc gccgaggccc gcgacctgct gaaggcgatg cctcaggcct atggcgccga
     2641 cctgcgtctg tcgcctcgca tgaagctgat cctggactcg gcgcaggccg aggccaagcg
     2701 cctgcaggat caattcgtca gcacggagca cctgctgctg gccattgcca ccgagagcgg
     2761 ccgctccccc gccgcgcaga tgctgcagcg actcggcgcc acgaaggacg cgctctacaa
     2821 ggcgttgacg gacgtccgcg ggaatcagcg cgtgacgtcc cagaatccgg aatccaccta
     2881 cgaggcgctc gtgcgctacg gccgcgacct cacggagctg gcgcgcgacg gcaagctcga
     2941 tccggtcatc gggcgcgacg aggaggtgcg gcgcgtcatc caggtcctct cgcgccgcac
     3001 gaagaacaac ccggtcctca tcggcgagcc gggcgtcggc aagacggcga tcgtcgaggg
     3061 gctcgcgcag cggatcgtgc gaggcgacgt tccggagggg ttgaagaaca agaagatctt
     3121 cgcgctcgac atgggagcac tggtggccgg cgcgaagtat cgcggcgagt tcgaagagcg
     3181 gctgaaggcc gtcctcaagg agatcgcgga gtcggagggc cagatcgtcc tgttcatcga
     3241 cgagctgcac accgtcgtcg gcgcgggcgc ggcggaaggc gcgatggacg cgtcgcagat
     3301 gctcaagccg atgctcgcgc gcggcgagc
//
LOCUS       DBNGSH010000980         5176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4042158, whole genome shotgun sequence.
ACCESSION   DBNGSH010000980 DBNGSH010000000
VERSION     DBNGSH010000980.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4042158"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="AC16CY_22395"
     CDS             <1..1821
                     /locus_tag="AC16CY_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356619.1"
                     /translation="TEILNGAVPGIDFFDPTRPLQYYASAAGLGLFGHQLLAEALLCV
                     TLLSAGAALVFFLGLEISGAVSLGAMASLFVVAILPRLYSYPKIIVPALALLFLWRYV
                     DRPAPARLAAVALATALSFYSRFDYGMWVAFAVGASLLARHWQAWRQLATAAMQYACV
                     VVLLCGPYVAMQVAMGGGLTSGPGSGRLGHLLKGEDVVSLGLPSIPSERPLLWFRPAG
                     PLATVRWVPNITAENRLMLERQYSLRMVRVVDEHARQYVLVDRSPATLARLIVDPSVE
                     ATTNIDDRGGILREPPWSVLRRWLRVPVVESPLLNQNDAMIWLYDALFLTPVLAAAVL
                     AVRAARRRAAEGETPKVLSVIVLSVLFNIFLIRGNLDSHLPDVIVPAALLWAWMMRGA
                     YQQLKAGAPARFGVATVALVLLWLTVDVYAGSVNQLTASDLFSTPRQAARRLYGTIRD
                     LRRDPLEQFAPPGATGLRALTRYVNRCTRETDRLLVLGYQPEMFFYANRRIGGGNVVY
                     QANLGAAPRQQATIVARLQRESVPIVILPANRVAELEQVYPIVKDYVYERYELAHESG
                     FGEDRLFRVLVDRRASFSTVDPELGLPCFTGSAVAASATP"
     gene            complement(1784..2551)
                     /locus_tag="AC16CY_22400"
     CDS             complement(1784..2551)
                     /locus_tag="AC16CY_22400"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR3356620.1"
                     /translation="MSTAGEWSVTVVVPTLREVENISPLVERVDAVRRATHLNLELLF
                     MDDDSRDGTVERVRALALDWVHVVVRTGEKGLSKAVLDGLGRSGRDVLVVMDADLSHP
                     PEKIPELIDALRQGADMAVGSRFVEGGSTDDDWGLFRWINSRVATWLAAPLTTLHDPM
                     SGFFALRQSTFRAGHAFNPVGYKICLELLVKCRCRSVAEIPIHFTDRTLGKSKLSLKE
                     QFNYLRHVRLLYIHKFGTWSPWSPRIREWRSQRPPSP"
     gene            complement(2579..2866)
                     /locus_tag="AC16CY_22405"
     CDS             complement(2579..2866)
                     /locus_tag="AC16CY_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356621.1"
                     /translation="MASNFNDPPVDPPADPPGDQSAAAPAPPQPAGLARFNSCRWQQP
                     AEEGNAAFCTHREVKPYAGTAGFDPEAWCVDCQYYKLRRVPKKRPPDEYRY"
     gene            complement(2847..4013)
                     /locus_tag="AC16CY_22410"
     CDS             complement(2847..4013)
                     /locus_tag="AC16CY_22410"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3356622.1"
                     /translation="MANAPEPVALAYEPAPFDPSPIRFDALSLDTRLLSGIRDLGWEE
                     TRPVQSGVIPLALAGGDVIASAETGTGKTAAFLVPILQRFLNEPAPQPPRTRTLILAP
                     TRELAVQIEDQVQGLTYHTAISSVAVFGGVPMDGQERALRAGVDIVVATPGRLMDHMR
                     HASIDFSGLEVLVLDEADRMLDMGFWPDVQRILSALPASRQTLLFSATMPGEVLKLTT
                     EFLREPDYIQVGRRGGPAQTISHNVQTIAAGQKTEWLARWLKHDATGPVLIFCRTKIG
                     AERLAQRLSGMGIRAASLHADRTQQQRMAAVEGFRTGTYPVLVATDVAARGLDIDGIA
                     HVVNYEVPDTPEAYVHRVGRTGRAEAAGNALTLVAPEEIRALRALEKAVGVQLQ"
     gene            complement(4104..4322)
                     /gene="infA"
                     /locus_tag="AC16CY_22415"
     CDS             complement(4104..4322)
                     /gene="infA"
                     /locus_tag="AC16CY_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3356623.1"
                     /translation="MSKDDLIDMQGTVVAVHSGGLYRVQCDAGHEVLAQLSGRMRRFR
                     IKVVPGDRVTVGVSPYDPVRGIITFRAR"
     gene            complement(4476..>5176)
                     /locus_tag="AC16CY_22420"
     CDS             complement(4476..>5176)
                     /locus_tag="AC16CY_22420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3356624.1"
                     /translation="EPLEEPVEFPQWNRTWPVRLVRRVSQATWILPMARVFAHIRVEG
                     LEHLRGLDGPVVFASNHQSHMDVPVILASMPGRWRARVAPAMAKEFFKAHFFPAAFSW
                     RERFTNSLNYYLAAFYFNAFPLPQREAGARQTLKYMGELTGKGWSILIFPEGERSNGG
                     ILPFRGGIGMIGSRLDIPIVPVRIEGVNRVLPVQGTFARPGRVRVAFGAPIRLRGDDY
                     AAMASTVEEAVRAL"
BASE COUNT          801 a         1733 c         1712 g          930 t
ORIGIN      
        1 accgaaatcc tgaacggagc ggtccccggc atcgatttct tcgacccgac tcgccccctc
       61 cagtattacg cgtccgccgc gggactcggg ctcttcggac accagttgct ggccgaggcg
      121 ctcctctgcg tgacgctgct gtccgccggg gcggccctgg tcttcttcct cggtctcgag
      181 atatccggcg cggtgtcgct tggcgcgatg gcgtcgctct tcgtcgtcgc cattcttccc
      241 cggttgtact catacccgaa gatcatcgtt cccgccctgg cccttctgtt cctgtggcgc
      301 tacgtggacc ggcccgcgcc ggcgcgcctg gccgcagtcg cgctcgcgac cgcgctgtcc
      361 ttttattctc gattcgacta cggcatgtgg gtcgccttcg ccgtcggcgc atccctgctc
      421 gcacggcatt ggcaagcgtg gcggcaactg gcgactgcgg cgatgcagta cgcctgcgtg
      481 gtcgtgctcc tgtgcggccc gtacgtcgcg atgcaggtgg cgatgggggg cggactcacc
      541 agtggacccg ggagcggacg actgggacat ctgctcaagg gcgaggacgt cgtgtcgctc
      601 gggttgccga gcatcccctc ggaacggccg ctcctgtggt tccgcccggc gggtcccctg
      661 gcaaccgtcc gatgggtccc gaacatcacc gcagagaatc gactgatgct cgagcggcaa
      721 tactcattgc gcatggtgcg ggtcgtcgat gaacacgcca ggcagtacgt tctggtcgat
      781 cggtcgccgg cgacgctcgc gcgtctcatc gtcgatccct cggtcgaagc caccaccaac
      841 atcgacgatc ggggcggcat cctccgggaa ccgccgtggt cggtgctgcg ccgctggctt
      901 cgtgtgcccg ttgtcgaatc tccgcttctg aaccagaacg acgcgatgat ctggctctac
      961 gacgcgttgt ttctcacgcc ggttcttgct gccgccgtgc tcgcggtacg cgccgcgagg
     1021 cgccgcgctg cggaaggaga aacaccgaag gtcctgtcgg tcatcgtgct cagcgtcctc
     1081 ttcaacatct tcctcattcg cgggaatctc gattcgcacc tgcccgacgt gatcgtgccg
     1141 gccgcgctgc tgtgggcctg gatgatgcga ggcgcctacc agcagttgaa ggccggggcg
     1201 ccggcgcgat tcggcgtggc gaccgtcgcc ttggtgttgc tgtggctcac cgtggacgtc
     1261 tacgccgggt cggtgaatca gctgacggcg agcgatctgt tttcgacgcc gagacaggcc
     1321 gcgaggcgtc tctacggcac gattcgcgat ctccggcggg atccgctcga gcaattcgcg
     1381 ccgcccggcg ccaccgggtt gcgcgcgctg acccggtacg tgaatcgatg caccagggag
     1441 accgaccgcc tgctcgtgct cgggtatcag ccggaaatgt tcttctacgc caatcgacga
     1501 atcggcggcg gcaacgtggt ctaccaggcg aacctgggcg ccgcgccgcg gcagcaggcg
     1561 acgatcgtcg cccggcttca acgcgagagc gttccgattg tcatccttcc cgccaatcgg
     1621 gtggcggagc tggagcaggt gtatccaatc gtgaaggact acgtgtacga gcgttacgag
     1681 ctcgcgcacg aatcgggctt cggcgaggac cggctctttc gcgtgctcgt cgatcggcgg
     1741 gcgtcgttct caaccgtcga tccggagctc ggcttgccct gcttcacggg ctcggcggtc
     1801 gctgcgagcg ccactccctg atccgcgggg accacgggct ccacgtgccg aacttgtgaa
     1861 tgtacagaag gcgaacatgc ctgagatagt tgaactgctc cttgagcgac agcttgctct
     1921 ttccgagtgt tcgatccgtg aagtggatcg ggatttcggc caccgatcgg caccggcact
     1981 tcacgaggag ctcgaggcag atcttgtatc ccaccggatt gaacgcatga ccggctctga
     2041 acgtcgactg ccgcagtgca aagaagccac tcatcgggtc gtgcaacgtc gtcagcggcg
     2101 cagccagcca tgtggcgacg cggctgttga tccatcggaa gagtccccaa tcgtcgtcgg
     2161 tggagccgcc ttcgacgaaa cgggatccca cggccatgtc cgcgccttgc ctcaacgcgt
     2221 cgatcagctc ggggattttt tccggcggat gactcaggtc ggcgtccatc acgaccagga
     2281 cgtcgcgccc tgaccggccg aggccgtcga gaacggcctt gctcaaccct ttttcccccg
     2341 tccgcacgac gacgtgcacc cagtcgagag ccagggcccg gacgcgttca accgtcccgt
     2401 cgcggctgtc gtcgtccatg aaaaggagtt cgagattcag gtgagtcgca cggcgcaccg
     2461 cgtctacgcg ttcgaccagg ggggagatgt tttccacctc ccgcagggtc ggcacgacga
     2521 ccgtcacgct ccattcgccc gcagtgctca ttcctgtggc ctgagccctg aggccttctt
     2581 agtagcgata ctcgtccggt ggtcgcttct tcggcacgcg tctgagcttg tagtactggc
     2641 agtccacgca ccacgcttcg gggtcgaacc ccgcggtgcc tgcgtacggc ttcacctcgc
     2701 ggtgcgtgca gaacgcggcg tttccttcct cggccggctg ctgccagcgg caggaattga
     2761 atcgtgcgag cccggccggc tgcggcggcg cgggagcggc cgccgactga tcgccgggcg
     2821 gatcggccgg cggatcgacg ggcgggtcat tgaagttgga cgccaacggc tttctccagc
     2881 gcgcgcaggg cgcgtatctc ttcgggggcg accagcgtca acgcgttgcc agccgcctcg
     2941 gcgcgcccgg ttcgtccgac gcggtgcacg tacgcctccg gcgtgtcggg cacctcgtag
     3001 ttgaccacgt gggcgatccc gtcgatgtcg agcccgcggg ccgcgacatc agtggccacc
     3061 agcacgggat acgtccccgt ccggaagccc tcgactgccg ccatccgctg ctgctgcgtt
     3121 cgatcggcgt gcagcgatgc cgcgcgaatg cccatcccgc tcagccgctg cgcgaggcgc
     3181 tcggcgccga ttttcgtccg gcagaagatg aggaccggcc ccgtcgcgtc gtgtttcagc
     3241 caacgggcga gccattccgt cttctgccct gcggcaatcg tctgcacgtt gtgcgagatc
     3301 gtttgcgccg ggccgccgcg gcgcccgacc tggatgtagt cgggctctcg caggaactcg
     3361 gtcgtgagct tgaggacctc gcccggcatc gttgccgaga acagcagcgt ctgacgcgac
     3421 gccggcaggg ccgagagaat ccgctggacg tccggccaga agcccatgtc cagcatgcga
     3481 tcggcttcgt cgagcacgag gacctcgagg ccggagaaat cgatggacgc gtgccgcatg
     3541 tgatccatca accgtccggg tgtcgcgacg acgatgtcca ctccggcgcg gagcgcgcgc
     3601 tcctgcccgt ccatcggcac gccgccaaat accgccacgc tcgagatggc cgtgtggtag
     3661 gtcagtccct gaacctggtc ttcgatctgc accgccagct cgcgcgtcgg cgcgaggatg
     3721 agcgtgcgcg tgcgcggcgg ctgcggcgcc ggctcgttga ggaatcgctg gagaatcggc
     3781 accaggaagg ccgccgtttt tccagtgccg gtttcggcgc tcgcgatgac gtcgccgccg
     3841 gcgagcgcga gcggaatcac gccgctctga accggccgcg tctcctccca cccaaggtcg
     3901 cggatgccgc tcagcaggcg cgtgtccagc gagagcgcgt cgaagcggat cggcgaagga
     3961 tcgaatggcg ccggctcgta ggcgagcgcc accggctcgg gcgcgttcgc cacgatggat
     4021 ctcggtcccc ggcgacgtgg cgggcgtcct cggccgcggc gtgtctgtgg tgatgtggag
     4081 ccgtgcttga tcaatgggat ggcttatctg gcgcggaacg tgatgatgcc cctgaccggg
     4141 tcatacgggc tgacgcccac agtcacgcga tcacctggaa cgactttgat tcggaagcgg
     4201 cgcatccggc cgctcaactg tgcgagtact tcatgcccgg catcgcactg cacacggtac
     4261 agcccacccg agtgcacggc gactactgtt ccctgcatgt cgatcagatc gtctttgctc
     4321 aataccccat ccttgcggtc ggtgacgggc tcgtggcgac cccgtgactg gttggctgtg
     4381 tccgggcgcg ctggggcgcg ctcagggcag caatcagggt gccacactgg caagcatcat
     4441 gcaagttatg cacgttcagc ggttagaaga cgcaactaca gggcgcgaac ggcctcctcg
     4501 accgtcgaag ccatcgccgc gtagtcgtcg ccgcgcaggc gaatcggggc gccaaatgcc
     4561 acgcgcacac ggcccgggcg ggcgaaagtg ccctggacgg gaaggacacg attgacgccc
     4621 tcgatgcgga ccgggacgat ggggatgtcg agccgggagc caatcatgcc gatcccgccc
     4681 cggaagggca ggatgccccc gttcgaccgc tcgccctcgg ggaagatcag gatcgaccac
     4741 cccttgcccg tcagctcacc catgtatttc aacgtctgcc gggcgcccgc ctcccgctgc
     4801 ggcagcggaa acgcgttgaa gtaaaaggcg gccaggtaat agttgagcga gttggtaaac
     4861 ctctcgcgcc aggagaatgc cgcggggaag aagtgggctt tgaagaactc cttggccatt
     4921 gccggcgcca cgcgcgcccg ccatcgtccc ggcatcgagg cgaggatgac cggcacgtcc
     4981 atgtggctct gatggttcga cgcgaagacg acggggccgt cgaggccgcg caggtgttcg
     5041 agcccctcga cgcggatgtg cgcgaacacg cgggccatgg ggagaatcca cgtggcctgc
     5101 gacacgcgcc ggaccaggcg gaccggccac gtccgattcc actgcggaaa ctccaccggc
     5161 tcctcgagcg gctcgc
//
LOCUS       DBNGSH010000981         5930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2514328, whole genome shotgun sequence.
ACCESSION   DBNGSH010000981 DBNGSH010000000
VERSION     DBNGSH010000981.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2514328"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..2597)
                     /locus_tag="AC16CY_22425"
     CDS             complement(165..2597)
                     /locus_tag="AC16CY_22425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3356625.1"
                     /translation="MERLWSDLRYALRSLARSPQFAVVAVLGLGVGLAVSSLMFSVVN
                     AYLFRPLPVKRGNEFVALGVQKPTSSGLRNLSYPNLIDIRDDLRSFEDVFAYSTVSPA
                     VRLGSMSERWGAQVVTGNFFQALGVQPRLGRALLPGDDVAGATPVAVISHAIWENRLA
                     SDPSILGRTISLNGSLFVVVGVAPEGFQGIDSLLRSDVWVAAASASKVGSGTDNRDSN
                     ALRVRGHLRPGVGMKTAQAEVDTLSVRLRERYPAENAGLRILVLPERRTRPLIDAGGL
                     VPIVGSMLIGLGIVVLINTCGSVMGLLLARSVERQREVSLRMALGATSRQVIGASVLE
                     SVLVAMGGGLVASVVVAWGARVIATGVPLASGYRFDIHPDPRVLAFTFLIAAVAGAAI
                     GLVPALGAARADIRSSLGSGHGVTFDRRRTRARTRVVMVQFALVTVLLVTAGLFMSGA
                     SGTKNTDIGFATDSRILIPVSPGDNGYDETRGRQILDEALARASALPGVRSATLVWDV
                     PLGASSSSIEVRAQGQAADAPPRRVPYNVVGSKYFETMAIPLVRGRSFDAPGALAASA
                     AIINETLAESYWPGQDPIGRALIVAGEGDARGYDVIGVARNAKYNSLREAPRGFVYLP
                     YAQHYRSEMTLVLHAPVNLAATTADVRELVESIDPTLVANDVTTLDASIETNALGSVR
                     LGAGLLVVFGAFGATLAVFGLFGLLSYTTRLQRRDIGIRMALGATTAIVTVELLGGSL
                     RLAGRGMVAGFVLAVVVAVVLRGTVLGIGALDLAPFLTVPVLLAVVTLAAGYLPVRRI
                     LNENPMRALSHE"
     gene            complement(2620..3351)
                     /locus_tag="AC16CY_22430"
     CDS             complement(2620..3351)
                     /locus_tag="AC16CY_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3356626.1"
                     /translation="MTDSRRRTIRVATYNIHRCRGLDGRTMPARIAEVIRSIDPDLIA
                     LQEVIGASIRSAGQAEELGALLGMGWVMAPVRQRRGAQFGNVVFSRHPIVHHAHYDLS
                     WKTCEPRGCQRVDIALGEHRLHLYNVHLGTALLERRYQARRLGAIVNDRRVGVPKVVL
                     GDFNEWMKGLATQTLTERLQSIDLRTHLRRRRTYPGVFPVLHLDHIYYEGSVEVVKVE
                     LPRSRMSLIASDHLPLVADLRVQFT"
     gene            3377..3679
                     /locus_tag="AC16CY_22435"
     CDS             3377..3679
                     /locus_tag="AC16CY_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356627.1"
                     /translation="MAKLKSEIVLECPCCKASLVVDTNLRRIVSHTVPERADKPELND
                     AANILAKETARRESMFEQSFEAEKSRDDVLSRRFEEALKQARTEPVTRPTRDFDLD"
     gene            complement(3681..4391)
                     /locus_tag="AC16CY_22440"
     CDS             complement(3681..4391)
                     /locus_tag="AC16CY_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356628.1"
                     /translation="MPNSTRFPLPKTDPIVPPSLPLTSAERRLIAGLRTPAAVQGWLN
                     ALPYNHERGAETLRSFRGVVRTGTAHCLEAALSAAVILEQHGYPPLVLSFESIDLLDH
                     VIFVYRTRTGWGSVARSRDPGLHGRKPLFATPRALALSYFEGYIDFTGRIKAYAVVDL
                     RVMGNYDWRLSPRNVWKVEQVLLDWKHRRIASSDRKAEAMRRRYRAFRETHGYKPWNY
                     YKGRERWMPLPEEFARRG"
     gene            complement(4354..5208)
                     /locus_tag="AC16CY_22445"
     CDS             complement(4354..5208)
                     /locus_tag="AC16CY_22445"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3356629.1"
                     /translation="MRACAVLCLVVAAAAAACSPRTPAREYELTGQILAVRPERREVV
                     IKHEDIKGFMPGMTMPFTVKDTSLLTGKQPGDLVTATLVVGQTEAHLSALTATGHAAL
                     DAPPPAPVADVLSAGQAVADAALIDQDGKARPFSSFRKHRLVLTFIYTRCPLPDFCPL
                     MDRHFASIQKTIAERAEFSDVRLVTVTLDPAFDRPAILKAYSRRRGADPAVWSFLTGE
                     PADVSRFATQFGLYVEHDPGNPIDITHNLRTAVIDPQGRLVSVRSGNDWTPAELVAEL
                     NAVPATED"
     gene            5296..>5930
                     /locus_tag="AC16CY_22450"
     CDS             5296..>5930
                     /locus_tag="AC16CY_22450"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3356630.1"
                     /translation="MRKTFGQTVAVEAMDLVVPRGALYGVIGPNGAGKTTCIRMIMSI
                     LFADSGELSVLGRRSALEAKDRIGYLPEERGVYRKMKVGAFLTYVARLKGLSQDDAED
                     RVPELLRKVGLPETEHKRCEDLSKGMLQRIQFVAAVIHKPDLLILDEPFSGLDPVSVR
                     VLRELVLEEHRRGATILLSTHVMVHAEEICQQVVMIHQGRKVLDEPVAGLRR"
BASE COUNT         1089 a         1963 c         1925 g          953 t
ORIGIN      
        1 cactcgaggc cgagagcgtg aatacacgac ggggctacgg cttcgccgtg cagttcgtca
       61 gtatgacgga cgacgtccgt gcggcgcttg aacgcgtcat cgaatggcgg gtcaccgcgt
      121 tgtggcacga cgaacggagg cgcgacgagt gacagtgatt tcagctattc gtgagagagt
      181 gccctcatcg gattctcatt gagaatgcgg cgcaccggaa ggtagcccgc cgcgagcgtg
      241 acaacagcaa gcagcacggg aaccgtgaga aacggcgcga ggtcgagtgc gccgattcca
      301 agcaccgtcc ctcgaaggac gactgccaca acgacggcga ggacgaagcc agccaccatt
      361 cctcggccgg ccagacgaag gcttcccccc agcagctcca ccgtcacaat ggcggtggtg
      421 gcaccaagcg ccatcctgat gccgatgtct cgcctctgca gccgcgtcgt gtaactcaag
      481 aggccgaaca gcccgaacac cgccagggtt gctccgaacg cgccgaagac aaccagaagt
      541 ccggcgccaa gcctgaccga gccgagcgcg ttcgtctcga tcgatgcgtc gagcgtggtc
      601 acgtcgttcg cgacgagcgt cggatcgatt gactccacca gctcccgcac gtccgccgtc
      661 gtcgccgcaa ggttgaccgg cgcatggagc acgagcgtca tctccgaccg gtagtgctgc
      721 gcgtacggca ggtagacgaa gccgcgcggg gcctcacgga gcgagttgta ctttgcgttc
      781 ctcgccacgc cgatcacgtc gtagccgcgg gcgtcacctt cgccagccac gatgagcgcc
      841 cggccaatcg gatcctggcc cggccagtac gattcggcca gggtttcgtt gatgatcgcc
      901 gcgctggctg cgagggcgcc cggtgcgtca aagctgcgac cccggaccag gggaatcgcc
      961 atcgtttcga aatacttcga tccgactacg ttgtagggca cacggcgtgg aggggcgtct
     1021 gcagcctggc cttgcgctcg aacctcgatc gaactgctgc tcgcgccaag gggcacgtcc
     1081 cacaccagcg tggccgagcg cacacccggc agggcggatg cgcgcgccaa ggcctcgtcg
     1141 aggatctgac ggcctcgggt ttcgtcgtag ccgttgtcgc ccggagatac gggaatcagg
     1201 atccggctgt ctgttgcaaa cccgatgtcc gtgttcttcg tgccgcttgc accgctcatg
     1261 aagagccctg ccgtgaccaa cagcacggtc accaaggcga actgcaccat cacgacgcgt
     1321 gtccgcgcgc gcgttcgacg acgatcgaac gtcacgccgt gcccgcttcc gagactgctt
     1381 cgaatgtccg cgcgcgctgc gccgagtgcc ggcacgagcc cgatggcggc ccccgcgaca
     1441 gcggcgatca gaaaagtaaa tgccaacacc ctgggatccg ggtggatgtc gaaccgatag
     1501 ccggaagcca gtggcacgcc agtcgcgatg actcgagctc cccatgcgac gacgaccgac
     1561 gcgacaagcc caccgcccat ggcaaccagt acgctttcga gaacagacgc tccaatcacc
     1621 tgcctcgaag tcgcgcccag agccattcgc agcgacacct cccgctggcg ctcgaccgag
     1681 cgcgctagca ggaggcccat cacgcttccg cacgtgttga tcaacacgac gatgccgaga
     1741 ccgatcagca tggagccgac gatcggcacg agcccgccgg catcgatcag cggacgcgtc
     1801 cgtctttccg ggagaaccaa tatacggagg cctgcgtttt cagccggata tcgttcgcgc
     1861 aggcgcactg agagggtgtc gacctccgcc tgcgccgtct tcatcccgac gccaggacgc
     1921 agatgccctc ggacccgaag ggcgttgctg tcgcggttgt cggtcccgct tccaaccttg
     1981 gacgcgctcg cggcggcgac ccagacgtcc gaccgtagca acgagtcgat tccttgaaac
     2041 ccttcgggag ccacgccgac gacgacgaac agggagccgt tgagggagat cgtgcgtccg
     2101 aggatggaag gatcggaggc caaccggttc tcccaaatcg cgtggctgat caccgccacc
     2161 ggcgtggcgc cggcgacgtc atcgccgggt agcagcgcac ggccaagccg cggctgaaca
     2221 ccgagggcct ggaagaagtt cccggtcacg acctgcgcgc cccagcgctc tgacatgctt
     2281 cccaatcgaa cggcagggct cacagtcgaa taggcaaaca cgtcctcaaa cgaacgcaga
     2341 tcgtcccgga tgtcgatgag attcgggtac gaaagattcc ggagtccgct cgacgttggc
     2401 ttctggactc ccaacgccac gaactcgttg ccgcgcttca cgggcagcgg ccggaacagg
     2461 tacgcattga cgacgctgaa catcaggctg ctgacggcga gcccgactcc caggccaaga
     2521 accgccacga cggcaaactg cggcgaccgg gccagcgacc gcagcgcgta cctcaggtca
     2581 ctccacagtc gttccatgac tctctccttt gccgttgccc tacgtgaact gcaccctcag
     2641 atccgccacg agcggcaggt ggtcggaggc gatcaacgac attctgctgc gcggcagttc
     2701 gaccttgacg acctccaccg agccctcgta gtagatgtgg tccaggtgta agacaggaaa
     2761 cacgccgggg taggttctcc gccgccgcag gtgcgttcgc aggtcgatgc tctgcagccg
     2821 ctccgtcagc gtctgcgtcg ccagcccttt catccactcg ttgaagtcgc ccaggaccac
     2881 cttgggtacg ccgacgcggc ggtcgttcac gatcgccccc agcctccggg cctggtaccg
     2941 ccgctccagg agtgccgtgc cgaggtgaac gttgtagagg tgcagtcgat gctcgcccag
     3001 cgcgatgtca acccgctggc atccgcgggg ctcgcacgtc ttccacgaca ggtcgtagtg
     3061 cgcgtggtgc acgatggggt gccggctgaa cacgacgttg ccaaactgcg ctccccgtcg
     3121 ttgccggacc ggcgccatca cccatcccat tccgagcagg gcccccagtt cttcggcctg
     3181 ccccgccgag cggatactgg cgccgatgac ctcctgcagc gcaatcaggt cggggtcgat
     3241 gcttcggatg acctcggcga tgcgcgcggg catggtgcgg ccgtccagtc cccggcaccg
     3301 gtggatgttg taggtggcaa cacgaatcgt ccgccgtcgc gaatcagtca cgctggatgg
     3361 tggttagaat acgtgtatgg cgaagctgaa gagcgaaatc gtgctcgaat gcccttgctg
     3421 caaggcatcg ctggtggtcg atacgaacct ccgccggatc gtgtcgcaca ccgtgcccga
     3481 acgcgcggac aaacccgaac tgaacgacgc cgcgaacatc ctggccaagg aaaccgcccg
     3541 tcgggaatcg atgttcgagc agtcgttcga ggcagagaag tcccgagacg acgtgctgtc
     3601 caggcgcttc gaggaagcgc tgaagcaggc caggaccgag ccggtcacga gaccgacccg
     3661 ggacttcgat ctcgactgac tcaaccacgt cgggcgaact cctccggcag cggcatccat
     3721 cgctcccggc ctttgtagta attccacggc ttgtagccgt gcgtctctcg aaacgcccgg
     3781 taccgccgtc gcatcgcctc cgccttccgg tccgacgagg cgatgcgccg gtgcttccag
     3841 tcgagcagca cctgctcgac cttccacacg ttgcgcggcg acagccgcca gtcgtagttg
     3901 cccatgactc gcagatcgac gaccgcgtac gccttgatgc ggcccgtgaa gtcgatgtag
     3961 ccctcgaagt agctgagggc gagcgctcgc ggcgtggcga agagcggctt gcgtccgtgc
     4021 aggccggggt cgcgcgagcg tgcgaccgat ccccagccgg tccgtgtccg gtagacgaag
     4081 ataacgtgat cgagcaggtc gatcgactcg aaactcagga cgagcggagg gtacccgtgc
     4141 tgctccagga tgacggcggc cgacagcgcc gcttcgaggc aatgggcggt gcccgtcctg
     4201 acgacgcctc gaaaactgcg cagcgtctcg gcgcccctct catggttgta cggcagcgcg
     4261 ttcagccatc cctgcaccgc ggccggcgtg cgtagcccgg cgatcaggcg gcgctccgcg
     4321 ctcgtcagcg gcagcgacgg cgggacgatc gggtcagtct tcggtagcgg gaaccgcgtt
     4381 gagttcggca acgagttcag ccggtgtcca gtcattaccg ctgcgcaccg agaccaggcg
     4441 gccctgcgga tcgatgaccg cggtccgcag gttgtgtgtg atgtcgatcg ggttccccgg
     4501 gtcgtgctcg acgtacaggc cgaactgcgt ggcgaagcgg ctcacgtcgg ccggctcgcc
     4561 cgtcagaaag gaccagacgg cggggtcggc tccgcggcgg cgcgagtacg ccttcaggat
     4621 ggcgggtcgg tcgaatgccg ggtcgagcgt caccgtcacg agccgcacgt ctgagaactc
     4681 cgcgcgttcc gcgatcgtct tctgaatcga cgcgaagtga cggtccatca gaggacaaaa
     4741 atcagggagc gggcagcgcg tgtagatgaa ggtcagcacg agacgatgct tcctgaagct
     4801 cgagaagggc cgagccttac cgtcctgatc gatcagcgcc gcgtccgcga cggcctggcc
     4861 agcggagagg acatcggcga ctggtgccgg cggaggcgcg tcgagcgccg cgtggccggt
     4921 ggccgtcagc gccgataggt gcgcctcagt ttggccgacg acgagcgtgg cggtgacgag
     4981 gtcgccgggc tgcttgcccg tcaggagcga ggtgtccttc acggtgaacg gcatcgtcat
     5041 gccgggcatg aagcccttga tgtcctcatg cttgatcacg acctcccggc gctcgggccg
     5101 caccgccagg atctgcccgg tgagctcgta ttcccgcgcc ggggtccgcg ggctgcacgc
     5161 cgcggccgcc gcggcaacca ccagacacaa gaccgcacac gcacgcatgt ctcgctactg
     5221 taccaacttt tggcgcgtga tataacgcca gtctcggaga acacaacggt ggaaccagcg
     5281 ctcgttctga agggtgtgag aaagacgttc ggccagacgg tcgcggtcga ggccatggac
     5341 ctcgttgttc cgcgaggcgc gctgtatggc gtcatcggac ccaacggcgc agggaagacg
     5401 acctgcatcc ggatgatcat gtcgatcctg ttcgcggact cgggcgagct gtcggtcctg
     5461 ggacgccggt cggcgctcga ggcgaaagat cggatcggat acctccccga agagcgcggc
     5521 gtgtaccgca agatgaaggt cggcgcgttc ctgacctacg tcgcacggct caaggggttg
     5581 agccaggacg acgctgaaga ccgcgtgccg gagctcctcc gaaaagtcgg cctgccggaa
     5641 accgagcaca aacgctgcga ggatctgtcg aaggggatgc tgcagcggat ccagttcgtg
     5701 gcagccgtca tccacaaacc ggacctgctc atcctcgacg agccgttcag cgggctcgat
     5761 cccgtgagcg tccgcgtgct tcgcgagctc gtgctcgagg agcatcgccg cggcgccacg
     5821 attctcctgt ccacccacgt catggtgcac gccgaagaga tctgccagca ggtggtgatg
     5881 atccatcagg ggcgcaaggt gctcgacgag cccgtggcag ggctgcgccg
//
LOCUS       DBNGSH010000982         5306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_307487, whole genome shotgun sequence.
ACCESSION   DBNGSH010000982 DBNGSH010000000
VERSION     DBNGSH010000982.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_307487"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..513)
                     /locus_tag="AC16CY_22455"
     CDS             complement(271..513)
                     /locus_tag="AC16CY_22455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356631.1"
                     /translation="MTKGRLLDRIRREFQDHPGIAVTLRQAQLLWSLDKRRCTEAFDD
                     LTAEGFLRQVEDVYVWVDAPVPQFRRPVAMRAYSPD"
     gene            667..1101
                     /locus_tag="AC16CY_22460"
     CDS             667..1101
                     /locus_tag="AC16CY_22460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3356632.1"
                     /translation="MDTPVRLLVVDPSAANLASMASLAEVEGVDVRTAVDASGAVEQC
                     RLGYLDLVMIDAGTDGAAGSRLLQAIRTQRPTVHAVLMSGPGARVARPSPDEDLACFD
                     KPVGLGPLRQLVRELRNRPRHVPNDLSDVSVGSDQLEHRGRH"
     gene            complement(1069..2490)
                     /locus_tag="AC16CY_22465"
     CDS             complement(1069..2490)
                     /locus_tag="AC16CY_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HIR3356633.1"
                     /translation="MPEYPLPLTSYPALDGAGLFEILRARAALEPFNVVAAAIFFCAI
                     VHTFLAARFTRIAHRLQYEHDERQRAAGLEPTPSVLAELLHFVGEVEVVFGLWVVPLL
                     AALALSKGGSVATHYLNDTVNFTEPLFVVVIMVLASARPVVALAETSLQRVAMLGRGT
                     AAAWWITILTVAPLLGSFITEPAAMTIGALLLSRRFFALEPSPRLKYGTLGLLFVNVS
                     IGGTLTHFAAPPVLMVARPWAWDTWFMLSHFGWRAAIAVAISTVAYRLLFWTDFRELA
                     GRTAVDDLQRADDLDGAGSSLPTPWWITTVHLVFMAWTVATSHYPVLFVGGFFFFLGF
                     TTVTAPYQGRLDLRPPLLVGFFLAGLVIHGGLQGWWIAPLLAGLSEQPLFVGSALLTA
                     FNDNALITYLGTLVPGLGDPLKLALVAGAVAGGGLTVIANAPNPAGQALLARFFGGAV
                     EPLKLLAGALLPTLVAAAMFQLI"
     gene            2595..3341
                     /locus_tag="AC16CY_22470"
     CDS             2595..3341
                     /locus_tag="AC16CY_22470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3356634.1"
                     /translation="MSAERVDLRADEGVLDVHVFVPDGGTGPWPAVILYMDAFGIRPQ
                     LASMAQRLASAGYVVAVPNLYYRTPFAPFAPNVVTTEGPERERFKGLIASINHAMVMR
                     DTNAVIGWLDSSPSVRKGSIAALGYCMGGGYAISAAGTFPDRVHLAASFHGGSLATDK
                     PDSPHRLAGRIRARVYVGAAEVDASFPRDQQDRLARALTEAGVTHEIETYAKTKHGFA
                     VTGHVVYDHEASELHWRRLLQLLKDAAAST"
     gene            3378..3932
                     /locus_tag="AC16CY_22475"
     CDS             3378..3932
                     /locus_tag="AC16CY_22475"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3356635.1"
                     /translation="MRLGIALVVGSVLAVAASSNAVAQGRGGAPACTTVACDVQGDWG
                     RGYALLIGVAEAMPEDKFGAKPTPAQESFGQRLMHVANIDLKLVSTLGGKTPAPMVNP
                     KAASKAEIVAALRQVSDYGSAVLKEFNDQQLAERVTSMPFLGPTASRLRVIYFAMTHS
                     QDIYGQLVVYLRLNGITPPASNRP"
     gene            complement(3948..4760)
                     /gene="truA"
                     /locus_tag="AC16CY_22480"
     CDS             complement(3948..4760)
                     /gene="truA"
                     /locus_tag="AC16CY_22480"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3356636.1"
                     /translation="MRFKLTIEYAGTRYSGWQVQRNARTVQGEIQRAVASLTGAREFE
                     LYGSSRTDAGVHALGQVAHLDLNTRLSPDALRRGINDELPADINVLDVQKVRHTFHAR
                     HSARGRSYIYQVARRRTAFAKPFVWWVKEDLDIARMRAAAARLVGFHDFRSFSDDDPQ
                     DKSTDVKLEALDVSVAGDLIVFRVEGSHFLWKMVRRLVGILVEVGRAGLTPADAAAML
                     TTPSELPARLTAPASGLFLERVRYEGDPPNGVGSHFLKTLVEPTPTRSVPEK"
     gene            complement(4783..>5306)
                     /locus_tag="AC16CY_22485"
     CDS             complement(4783..>5306)
                     /locus_tag="AC16CY_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR3356637.1"
                     /translation="FPKQKQVFGPSQVVGRIGQDQVISPQITLWNQQGSQVIWGTLMV
                     IPIEESLIYVRPLYLRSTGGRIPELTRVIVAYENRIVMEETLEAGLAQLFGGALAPVE
                     PAGRAPGRAAAGTPDAAKPPDAGPGGDLAALAAEARAHYERAVAAQRAGDWAAYGDEI
                     RQLGEVLQRMRGK"
BASE COUNT          932 a         1726 c         1841 g          807 t
ORIGIN      
        1 gccagcgcaa ggatcgcgac ggcggcgagc gagaggcgac gggccttcat atgacactcc
       61 acatccgggg gggcagaatt ctactgtgcc agagacggcc gccgagaaca aggacggcgt
      121 ggctcctgtg gaccctcgat caaatctgca tgtcgagggc ggattctgta aggggttggt
      181 gcctcgtatc aggacaaccc gttggccggg tcgggtaggc ggctgacgcc agaaggctgc
      241 cttcgcgcgt cgcgcttggg cgcccgggcc tcagtctggt gaatacgccc tcatcgccac
      301 cggccgccgg aactggggga ccggcgcgtc cacccatacg tacacgtcct cgacctgacg
      361 cagaaagcct tcggcggtca ggtcgtcgaa cgcctcggtg cagcgccgtt tgtcgaggga
      421 ccagaggagt tgcgcctgtc gcagcgtgac ggcaattccc ggatgatcct ggaactcgcg
      481 gcggattctg tccagcaggc gacccttcgt catacgtcct ccactgaagc gatcgcgccg
      541 ggccgtgcac gtgaagcggg gcaagcggcg gcgcgcagcc cgcgcaggtg agcaagaaca
      601 catccagtgc aattgtgtgt agacgagccg tgctcgaatg gaagtgtcct tgctatcact
      661 cactccgtgg acactcccgt caggctcctc gttgtcgatc ccagcgcggc caacctggcc
      721 tccatggcga gccttgctga agtcgaaggc gtagacgtcc gcacggccgt ggacgcgagc
      781 ggcgccgtgg agcagtgcag gctcgggtac ctcgatctgg tcatgatcga cgcgggcact
      841 gacggcgccg cgggcagccg tctgctgcag gcgatacgca cccagcgccc gaccgtccac
      901 gccgtgctga tgagcggacc cggcgcacgc gtggcgcggc catcgccgga cgaagacctc
      961 gcctgcttcg acaagccggt tggactcggt ccgctgcggc agctcgtccg ggagctccgc
     1021 aaccggccgc ggcacgtgcc gaacgatctc agcgacgtga gcgtgggatc agatcaactg
     1081 gaacatcgcg gccgccacta gcgtcggcag cagcgcgccg gcgagcagct tcaacggctc
     1141 caccgctcca ccgaagaacc tggcgagcaa cgcctgcccg gccgggttgg gcgcattggc
     1201 gatgaccgtc agacccccac cggccacggc accggcgacg agcgccaact tcaacggatc
     1261 gccgaggccg gggacgagcg tgccgagata ggtgatcagc gcgttgtcgt tgaacgcggt
     1321 gagcagcgcg gatccgacaa acagcggctg ctcggacagg ccagccagca gcggagcgat
     1381 ccaccagccc tgcagaccgc cgtggatgac gagtccggcg agaaagaagc cgacgagcag
     1441 cggcggccgt aagtccagac ggccttggta tggcgccgtc acggtcgtga atcccaggaa
     1501 gaagaagaag cctccgacaa acagtaccgg gtagtgagat gtggcgacgg tccacgccat
     1561 gaagacgagg tgaacggtcg tgatccacca tggcgtcggc agactcgagc cggcgccgtc
     1621 gaggtcatcg gcgcgctgga gatcgtccac ggctgtacgg ccggccagct cgcggaagtc
     1681 tgtccagaag agcaggcggt acgccacggt cgagatcgct acggcgatgg ccgcgcgcca
     1741 gccgaagtgg ctgagcatga accaggtgtc ccacgcccac gggcgcgcga ccatcaacac
     1801 cggcggtgcg gcaaagtgcg tcaacgtgcc gccgatcgag acgttgacga agagaagccc
     1861 gagcgtgccg tacttcagtc gtgggctcgg ttcgagggcg aagaacctgc gggacagcag
     1921 cagcgcgccg atcgtcatgg ccgccggctc ggtgatgaag gagccgagga gcggggcgac
     1981 cgtcagaatc gtgatccacc acgccgcggc cgtgccgcgg ccgagcatcg cgacgcgctg
     2041 aaggctcgtt tcagcgagtg ccacgacggg ccgcgccgac gcgagaacca tgatgacgac
     2101 cacgaacaag ggctcggtga agttcacggt gtcgttcagg tagtgggtcg ccacgctgcc
     2161 gcccttgctg agcgcgagcg ccgcgagaag cgggaccacc cagaggccga acaccacctc
     2221 gacttcgccg acaaagtgca ggagctcggc aagaacggag ggagtgggct cgaggccggc
     2281 tgcccgctgc cgctcgtcgt gctcgtactg cagcctgtgt gcgattcgcg tgaagcgggc
     2341 ggcgagaaag gtatgcacga tcgcgcagaa gaagatcgcg gcggccacga cgttgaacgg
     2401 ttcgagcgcc gcgcgtgcac gcaggatttc gaacagcccc gcaccgtcga gcgcggggta
     2461 cgacgtcaat ggaaggggat actcgggcac ctcgggtcgg tcctcgtcgg ttcctgcacc
     2521 atacccgagc cggccccatc ggccaagaac atccggcagg cacggtacaa tgctcgctct
     2581 cgagaggtga gcagatgagc gcggagcggg ttgacctgcg ggccgacgag ggggtcctcg
     2641 acgtccacgt gttcgtaccg gacggcggta cgggtccctg gccggccgtg atcctctaca
     2701 tggacgcctt cggcattcga ccccagctgg ccagcatggc ccagcggctt gcgtcggcgg
     2761 gatacgtcgt ggccgttccg aacttgtact accgcacgcc gttcgcgccg ttcgccccca
     2821 acgtggtcac gacggaagga ccagagcggg aacgcttcaa gggcctgatc gcgtcgatca
     2881 accacgcgat ggtgatgcgc gataccaacg ccgtgatcgg gtggctggat tcgagtccgt
     2941 ccgtgcggaa ggggagcatc gccgcgctcg gatactgcat gggcgggggc tatgccatca
     3001 gcgctgccgg cacgttcccg gatcgagttc atctggcggc gtcgttccac ggcggctcgc
     3061 tcgcgaccga caagccggac agcccgcatc ggctcgccgg ccggatacgc gcccgcgtgt
     3121 acgtcggcgc ggccgaagtc gacgccagct ttccccgcga ccagcaggat cggctcgcgc
     3181 gcgcgctgac ggaggccgga gtgacgcacg agatcgagac ctacgcgaag acgaaacacg
     3241 gtttcgcggt gacggggcac gtggtgtacg accacgaggc gtcggagctg cactggcggc
     3301 ggctgctcca gctcctcaaa gacgccgcag caagtacgta gtgtccggct tcagttgaac
     3361 caattgcagg aggcttcatg agactcggaa tcgcactcgt ggtcggatcg gtactggcgg
     3421 tggccgcctc gtcgaacgca gtggctcagg gcaggggagg ggcacccgca tgcacgacgg
     3481 ttgcgtgcga cgtgcagggc gattggggca ggggctatgc cctgctcatc ggcgtcgcgg
     3541 aggcaatgcc cgaggacaag tttggcgcca aaccgacccc ggcgcaggag agcttcggcc
     3601 agcgcctgat gcacgtcgcc aacatcgatc tgaagctcgt gagcacgctc ggcggcaaga
     3661 cgccggcgcc gatggtgaac ccgaaggcgg cctcgaaggc cgagatcgtg gcggcgctgc
     3721 ggcaggtgtc ggactacggg tcggcggtgc tcaaggagtt caacgatcaa cagcttgccg
     3781 aacgcgtcac gtcgatgccg ttccttgggc cgaccgcgag ccggctgcgg gtgatctact
     3841 tcgcaatgac gcacagccag gatatctacg gccagttagt cgtctatttg cgcctcaacg
     3901 gcatcacgcc gccggcaagc aacaggccgt agaaatgggg tcgggagtca tttctcgggg
     3961 acgctcctcg tgggcgtcgg ctcgacgagt gtcttcaaga aatgactccc gaccccgttc
     4021 ggagggtcac cctcgtagcg cacgcgttcc aggaacaagc cggaggccgg cgcggtcagg
     4081 cgcgccggca gctccgacgg cgtcgtgagc attgcggcgg cgtccgcggg cgtgaggccc
     4141 gcgcgcccga cttcgacgag gatccccacc agccgccgga ccatcttcca caggaagtga
     4201 gagccttcca cccgaaagac gatcaggtcg ccggccacgg agacatccag cgcctcgagc
     4261 ttgacatcgg tggatttgtc ctgcgggtcg tcgtcggaga acgaccggaa gtcgtggaac
     4321 ccgacgagcc gcgctgcagc cgcgcgcatc cgcgcgatgt cgaggtcctc cttcacccac
     4381 cagacgaacg gcttggcaaa cgccgtccgc cggcgcgcga cctggtaaat gtagcttcgg
     4441 cccctggcgc tgtgccgcgc gtgaaaggtg tgccgcacct tctggacgtc gagaacgttg
     4501 atgtcggccg gcagctcgtc gttgatgccg cgtcgaagcg cgtcaggtga cagccgcgtg
     4561 ttcagatcga ggtgagcgac ctgtcccagg gcgtgcacgc cggcgtcggt ccggctcgat
     4621 ccgtatagct cgaactcgcg tgcgccggtc agagaggcga cggcgcgctg gatctcaccc
     4681 tgcaccgtgc gcgcgttccg ctgcacctgc cagccgctgt agcgcgtgcc ggcgtactcg
     4741 atggtgagct tgaagcgcac gtcttctacc gcgcgtgcgc gatcatttac cgcgcatgcg
     4801 ctggagtacc tcgccgagct ggcggatctc gtcgccgtat gccgcccagt cgccggcgcg
     4861 ctgcgcggcg accgcgcgtt catagtgggc tcgcgcttcg gcggcgagcg ccgcaaggtc
     4921 gccgccagga cccgcgtccg gcggcttcgc cgcgtccggt gtgccggccg cggctcggcc
     4981 cggcgctctt ccggccggct cgacgggcgc cagggcgcct ccgaacagct gtgccagccc
     5041 ggcctcgagc gtctcttcca tgacgatgcg gttctcgtac gcgacgatga ccctggtcag
     5101 ctcggggatg cggccccccg tggagcgcag gtagagcggg cgtacgtaga tgagcgactc
     5161 ctcgatggga atcaccatga gcgtgcccca gatgacctgc gagccctgtt gattccagag
     5221 ggtgatctgc ggcgagatga cctgatcctg gccgatacgg cccacgacct ggcttggacc
     5281 gaacacctgc ttctgtttcg ggaact
//
LOCUS       DBNGSH010000983         2977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_987853, whole genome shotgun sequence.
ACCESSION   DBNGSH010000983 DBNGSH010000000
VERSION     DBNGSH010000983.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_987853"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="AC16CY_22490"
     CDS             complement(<1..1217)
                     /locus_tag="AC16CY_22490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356638.1"
                     /translation="MPPSHKGLQALFSYPLMSAIFDRRTRRVARGTSIVSGPISYTST
                     NKPAPLTPLEEAVLIVSTGLTGKVTMHDVPAKNDNGSNRFSAPLINVLARSASSIDNA
                     HAVSFFLINDEGTWLIKHFRNQEALAFLSKLPPRWEDWTEMDWIGAASLLKHKLYKER
                     LDFPRHWPYYFIWNRQISNRPGTTILLPIVDLTRQFINVVISLLSEEDGQRPLFVDDW
                     QRFRPRTLLDWGAWFGSLVGLVPNISFQIIGGARRARGNWLNNEYPAPIGFANTLRTD
                     YETFFQLQNLMLIAQAMGLGAWIHAAVGSPYVFERDPAKGKFGIEFRMQKPRRWRRWP
                     PLPTTLDNPIGIDGVLESLTPPYVKSMDAAVDQVIEEKYGRGGTYGDNSIFDRAYKDS
                     QHGDAFLKMASQRP"
     gene            complement(1231..2811)
                     /locus_tag="AC16CY_22495"
     CDS             complement(1231..2811)
                     /locus_tag="AC16CY_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR3356639.1"
                     /translation="MPTSFDVIVIGSGFGGAITARRLAEKGMRVLMLERGRRWEPAQY
                     PRKPGDAWIFDADQPARRNGWIDMRFFRRMTVAQAAGVGGGSLTYSSVAVEASPDVFT
                     RGWPAEITYAELKPYYDTVAQEMNLQVIPDGQLTQRFILAREAARNLGHSSRFSKAPL
                     AVSFSDDWNYQLEDPFNPKHSRQFVNAHGQRQGTCIHLGNCDIGCDVRAKNGLDVNYI
                     PRAEQRGAEVRPLHLVRYIEPRGGAYRVVFDRLDRGRLIAGDETAERVVLAAGSLGTT
                     ELLLRCRDQYGTLRALSPTLGTRWSANANFISMGAYSERDRVRQSTGPTIASLLDFTD
                     GSFRNERFVVEDDGFPNLLLSATKAYLDAGVRTRFGRHLLSELEEHLRDDTRLRGLMI
                     WLGAGMDAGDGRLHLRRRWLLPWKRVLNLAWEVERSEGVFNAMNDMQQNLTEATGGRP
                     RSLPTWTLLRSLLTLHPLGGCPMGATHDTGVVDHAGRVFGHRNLIVADGAIVPTPTVR
                     NPSHTIAALAERIAAHVS"
BASE COUNT          459 a          967 c         1010 g          541 t
ORIGIN      
        1 ggacgctggc tcgccatctt caggaaggcg tcgccgtgct ggctgtcctt gtacgcgcgg
       61 tcgaagatcg agttgtcgcc gtaggttccg ccccgcccgt acttctcctc gatcacctgg
      121 tccaccgcgg cgtccattga cttgacgtag gggggcgtca gcgactcgag cacgccgtcg
      181 atgccgatcg ggttgtcgag cgtcgtcggc aacggcggcc agcgccgcca ccgcctcggc
      241 ttctgcatcc ggaattcgat gccgaacttg cccttggccg gatcgcgctc gaagacgtac
      301 ggcgatccaa ccgcggcgtg aatccacgcg cccagcccca tcgcctgcgc gatgagcatc
      361 aggttctgca gctgaaagaa cgtctcgtag tcggtgcgca gcgtgttggc gaacccgatc
      421 ggcgccgggt actcgttgtt gagccagttg ccgcgtgccc gccgcgcgcc gccgatgatc
      481 tggaacgaaa tgttcgggac gaggccgacg aggcttccga accacgcgcc ccagtcgagg
      541 agcgtcctcg ggcggaatcg ctgccagtcg tccacgaaga gcggccgctg cccgtcctcc
      601 tcggacagga ggctgatgac gacgttgatg aactgccgcg tgagatcgac gatcggcaga
      661 aggatcgtcg tgcccgggcg gttcgagatc tgccggttcc agatgaagta gtagggccag
      721 tgcctcggga agtccaggcg ctccttgtac agcttgtgct tgagcagcga ggccgcgccg
      781 atccagtcca tctcggtcca gtcctcccag cgcggcggca gcttgctgag gaaggcgagc
      841 gcctcctggt tgcggaagtg cttgatgagc cacgtccctt cgtcgttgat caggaagaac
      901 gacacggcat gcgcgttgtc gatgctgctc gcggaccgcg ccagcacgtt gatgagcggc
      961 gccgagaacc ggttgctccc gttgtcgttc ttggccggaa cgtcgtgcat cgtcaccttg
     1021 cccgttagcc cggtggacac gatgagcact gcctcttcga gcggtgtgag cggcgcgggc
     1081 ttgttcgtgc tcgtgtagct gatcgggccc gacacgatcg acgtaccgcg cgcgaccctt
     1141 cgagtgcgcc gatcgaagat ggccgacatc agcggatacg agaacagcgc ctgcagcccc
     1201 ttgtgtgatg gaggcatgga atctcctctg ttacgagacg tgtgccgcga tgcgctcggc
     1261 gagcgcggca atcgtgtgcg agggattgcg gaccgtcggc gtcgggacga tcgcgccatc
     1321 ggcgacgatc aggtttcgat ggccgaacac gcggccggcg tgatcgacga cgccggtgtc
     1381 gtgagtggcg cccatgggac accctccgag cggatgcagc gtcaacaggc tcctgaggag
     1441 cgtccacgtc gggagcgacc gcggacgtcc gccggtcgct tcggtcaggt tctgctgcat
     1501 gtcgttcatg gcgttgaaca cgccctcgga ccgctcgacc tcccaggcca ggttcagcac
     1561 ccgtttccac ggcaacagcc atcgtctcct caggtgcagt cggccatcgc cggcgtccat
     1621 gccggctccc agccagatca tcagcccgcg aagacgggtg tcgtcgcgca ggtgctcttc
     1681 gagctcgctg agcagatgcc ggccaaacct cgtcctcacg ccggcatcga ggtaggcctt
     1741 ggtcgcgctg agcaggaggt tcgggaagcc gtcgtcttcg acgacgaatc gctcgtttct
     1801 gaagctgccg tccgtgaagt ccagcaggct ggcaatggtc ggaccggtgg actgccggac
     1861 gcggtctctc tccgagtacg cgcccatcga gatgaagttg gcgttggcgc tccagcgcgt
     1921 gccgagcgtg gggctgagcg cgcgcagcgt cccgtactga tcgcggcagc gcagcagcag
     1981 ctcagtcgtc ccgaggctgc cggcggcgag gacgacgcgc tcggccgtct cgtcaccggc
     2041 gatcaggcgt cccctgtcga ggcgatcgaa cacgacccgg tacgcgccgc cgcgcggctc
     2101 gatgtatcga accaggtgca gcggccgcac ctcggcgccg cgctgctcgg ctcgtggaat
     2161 gtagttgacg tccagcccgt tcttcgcccg cacgtcgcac ccgatgtcgc agttgccgag
     2221 atggatgcac gtgccctgtc gctgtccgtg ggcgttgacg aactgccgcg agtgcttcgg
     2281 attgaacggg tcctcgagct ggtagttcca gtcgtcggag aagctgacgg ccaggggcgc
     2341 cttcgagaac cggctggaat gccccaggtt gcgcgcggcc tcgcgcgcga ggatgaagcg
     2401 ctgcgtcagc tgcccgtcgg ggatgacctg caggttcatt tcctgggcca cggtgtcgta
     2461 gtagggcttc agctccgcgt aggtgatctc ggccggccag cccctggtga acacatcggg
     2521 gctggcttcc acggcaacgc tgctgtaggt cagcgatccc ccgccgacgc cggcggcctg
     2581 tgccaccgtc atgcgcctga agaagcgcat gtcgatccag ccgttgcggc gcgcgggctg
     2641 gtctgcgtcg aagatccacg cgtcgccggg cttcctcgga tactgtgcag gctcccagcg
     2701 gcggccacgc tccagcatca ggacgcgcat gcccttttcg gcgagccgcc gcgccgtaat
     2761 cgcgccaccg aagccgctgc cgatgacgat gacgtcgaaa gatgtgggca tgccgacgcc
     2821 tggtcgttgt cgcgttgtcg atcagcgccg cacgcgttgc tcgttcacgc gaatgtaggt
     2881 ggacggatcg ttgcggtcgt cccgccagcc gggatggtgg aactggacga cgtggtcggc
     2941 gttgtaggag gccaccgcct ctgtgaacct caacttg
//
LOCUS       DBNGSH010000984         2912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4043461, whole genome shotgun sequence.
ACCESSION   DBNGSH010000984 DBNGSH010000000
VERSION     DBNGSH010000984.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4043461"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="AC16CY_22500"
     CDS             complement(<1..1091)
                     /locus_tag="AC16CY_22500"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR3356640.1"
                     /translation="MQRSLEQIIAAYRTDLPLERASTIPAAWYTDARVSELERSTVFK
                     RSWQVAGRRDAVRAPGDYIACDLAGGEPIVVSRADDRVLRAFFNVCRHHAAAVVTDAA
                     GSARQFRCPYHGWTYALDGTLKGTPDFTGVCDFDRSSNGLVPIDCASSGQWVFVKPAQ
                     GGLSLERYLGPELSSGLEQLGLDRLHWMEQRRYTLDCNWKVFVDNYLDGGYHVPHLHK
                     GLDSVLDYSEYTIEIGERFCLQWSPMVKEGADERTGAVREGERAHYYWIYPNFMINCY
                     GGAMDTNLVIPRGVDKTEVVFDFYFADVSEPARARNLASIAVSEQIQEEDVSICVSVQ
                     RGLRSGGYDAGRLSVRREAGEHLFHRLLHA"
     gene            complement(1104..2057)
                     /locus_tag="AC16CY_22505"
     CDS             complement(1104..2057)
                     /locus_tag="AC16CY_22505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3356641.1"
                     /translation="MPVDPQCKAFLDILAGVGGKPIEALPPEEARQVSGGMINFAGPE
                     PPVARVENRSIPGPSQPIPIRVYWPSLDDRLPALVFFHGGGFVLCDLDSHDRLCRSLA
                     NESGCVVVSVGYRLAPEHKFPAAPEDAYAATCYVAAHASEFGIDSGRIAVGGDSAGGN
                     LATVVALMSRDRQGPSLRFQLLIYPVTDFYDESPSMHDYGRDHFLTRDAMNWFLKQYF
                     SSAADARLPYASPMNADVRGLPPAMIITAECDPVRDQGEAYADKLRRAGVSVELKRYD
                     GMVHPFFSLGGIVDAARTATKDAASAMEHALARAAPAGSGR"
     gene            complement(2067..>2912)
                     /locus_tag="AC16CY_22510"
     CDS             complement(2067..>2912)
                     /locus_tag="AC16CY_22510"
                     /inference="COORDINATES: protein motif:HMM:NF024946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR3356642.1"
                     /translation="VYSKIRVRVDSAFRSTAPDRAEFFYAKCGCYGLLPAASPNFDPD
                     APGPARAVNDLDFNQIDIFGEWAFGIFSAFGQLPLRWIQPRSLIPGPGGAFPNQSGLG
                     DVRAGIKMAVVETRDQSLTAQAQFYVPTGDASQGLGTNHTSFEPSLLYYRRLSDDIGL
                     ESQFGVWFPLDGSAGVPTSVEESFSGQVLNYGVGASFDVHREGRILFAPIVELVGWRV
                     LSGFQTSPTVAANPANGVNILDFKVGARVTVDGTHSVYGGFGHALTDATWYDDIVRIE
                     YRFSF"
BASE COUNT          506 a          955 c          994 g          457 t
ORIGIN      
        1 gcgtgcagca gacgatgaaa gagatgctct ccggcttccc gccggacgga caggcggccg
       61 gcgtcatagc cgcccgagcg caggccgcgc tggaccgaga cgcaaatcga aacgtcctcc
      121 tcctgaatct gctcgctgac ggcgatgctg gcgaggttgc gggcgcgagc cggctccgag
      181 acgtcggcga agtagaagtc gaacacgacc tcggtcttgt ccacgccgcg ggggatgacc
      241 aggttcgtgt ccatggcgcc gccgtagcag ttgatcatga agttcggata gatccagtag
      301 tagtgcgctc gctcgccctc gcgcaccgcg ccggttcgct cgtcggcccc ttccttcacc
      361 atcgggctcc actgcaggca gaaacgctcg ccgatctcga tcgtgtactc gctgtagtcc
      421 aggacgctgt cgagcccctt gtgcaggtgc gggacgtgat acccgccgtc gagatagttg
      481 tccacgaaga ctttccagtt gcagtcgagc gtatagcggc gctgctccat ccagtgcagc
      541 cgatcgaggc cgagctgctc gagacccgac gacagttccg ggccgagata gcgctcgagc
      601 gagagcccgc cctgcgccgg cttgacgaac acccattgcc cggacgaggc gcagtcgatc
      661 gggacgaggc cgttggagga gcgatcgaag tcgcagacgc ccgtgaagtc gggcgtccct
      721 ttcaacgtac cgtcgagcgc gtaggtccac ccgtggtagg ggcatcggaa ctggcgggcc
      781 gagccggcgg cgtcggtcac gaccgccgcg gcgtgatggc ggcagacgtt gaagaaggcc
      841 cgcaggacgc gatcgtcggc tcgcgagacg acgatcggct cgccgccggc gagatcgcag
      901 gcgatgtagt cgcctggcgc gcgcaccgcg tcgcgccgcc cggccacctg ccacgagcgc
      961 ttgaacaccg tgctgcgctc gagctcggag acccgggcgt ccgtgtacca ggccgcgggt
     1021 atggtggacg cccgctcgag cggcaagtcc gtccggtacg cagcgatgat ctgctcgagg
     1081 gagcgctgca tgtgagacgc gggctacctg ccggagccgg ccggtgccgc acgagccagc
     1141 gcgtgttcca tcgcggaggc ggcgtccttc gtcgccgtgc gcgcggcgtc cacgattccg
     1201 cccaggctga agaaggggtg caccatgccg tcgtagcgct tcagctcgac ggacacgccg
     1261 gcccgtcgca gcttgtccgc gtaggcctct ccctgatcgc ggaccgggtc gcactcagcg
     1321 gtgatgatca tcgccggcgg gagtcctcga acgtccgcgt tcatcggcga cgcgtacggc
     1381 aggcgggcat cggcggcgct cgagaagtac tgtttcagga accagttcat cgcgtcgcgt
     1441 gtcaggaagt gatccctccc gtagtcgtgc atggaaggac tctcgtcgta gaaatcggtg
     1501 acggggtaga tcagcagctg gaatctcaac gacggcccct ggcgatcgcg cgacatcagc
     1561 gccaccaccg tcgccaggtt tccgcccgcg ctgtcgccgc ctacggcgat gcggccggaa
     1621 tcgatcccga actcggaggc gtgtgccgcc acgtaacagg tcgccgcgta cgcgtcttcc
     1681 ggtgcggcgg ggaacttgtg ctcgggcgcc aggcggtagc ccaccgacac gacgacgcag
     1741 cctgactcgt tggcgaggct gcggcacagc cgatcgtggg aatcgagatc gcagagcacg
     1801 aagccgccgc cgtggaagaa gacgagcgcg ggcaggcgat catcgagcga gggccagtag
     1861 acgcggatcg gaatcggctg cgaggggccg gggatcgagc ggttctcgac cctggcgacc
     1921 gggggctccg gtccggcgaa gttgatcatc cctcccgata cctgccgggc ctcctcgggc
     1981 ggaagcgcct cgatcggctt gccgccgacg ccggcgagga tgtcgagaaa ggccttgcac
     2041 tgcgggtcga ctggcatagc ggcattctag aacgagaacc ggtactcgat tctcacgatg
     2101 tcgtcgtacc aggttgcgtc ggtgagcgcg tgcccgaagc cgccgtacac cgagtgcgtg
     2161 ccgtcgacgg tcacccgcgc cccgaccttg aaatcgagga tgttgacgcc gttggcgggg
     2221 ttggccgcga ccgtgggcga cgtctgaaat ccgctcagga cacgccatcc cacgagctcg
     2281 acgatcggcg cgaagaggat cctgccctcg cggtgcacat cgaagctcgc gccgaccccg
     2341 taattcagca cctggcccga gaagctttcc tcgacgctcg tcgggacgcc ggcagagcca
     2401 tcgagcggga accagacgcc gaactgggat tcgagcccga tgtcgtcgga aagccgcctg
     2461 taatacaaca gcgacggctc gaagctcgtg tgattggtac ccaacccctg cgaggcatcg
     2521 ccagtcggca cgtagaactg ggcctgcgcc gtcaggctct gatcccgggt ctcgacgacc
     2581 gccatcttga tgccggcgcg cacgtcgccg aggccggact gattcggaaa ggcgccgccc
     2641 ggtcccggta tcaacgacct cggctgaatc catcgcagcg gcagctggcc gaaggcggaa
     2701 aagattccga acgcccactc gccgaagatg tcgatctggt tgaaatcgag atcgttgacc
     2761 gctcgcgccg gcccgggtgc gtcgggatcg aagttcgggc tggccgcggg cagcaggccg
     2821 tagcagccac atttcgcata gaagaactcc gcccggtccg gcgcggtgct gcgaaacgcg
     2881 ctgtcgactc gaacacggat cttcgaatag ac
//
LOCUS       DBNGSH010000985         4397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4214164, whole genome shotgun sequence.
ACCESSION   DBNGSH010000985 DBNGSH010000000
VERSION     DBNGSH010000985.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4214164"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="AC16CY_22515"
     CDS             <1..306
                     /locus_tag="AC16CY_22515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HIR3356643.1"
                     /translation="ENPPSTIIDAVASGKIDVAIVWGPAAGYFVKNQRVPLAMVPVPS
                     AKGDLPFEFDIAMGVKIGNDALKTQLQKVVDAKQAEITKILKDYGVPLIDRKADSKR"
     gene            303..779
                     /locus_tag="AC16CY_22520"
     CDS             303..779
                     /locus_tag="AC16CY_22520"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3356644.1"
                     /translation="MTTRRPHGQFPPITLGAALAVFLSVLAGVVHERLPLLPDTSLAA
                     QAPADIYKDVYDGWKWWHVYCYRCHGTNAIATTLAPDLIDPNRRLTDAQFLKVVRDGA
                     PDKGMQAWDKLLDNKQIGQIHLYVRARTDKVLPPGRPDEVGPKGGPWVPPAGWPKR"
     gene            833..1324
                     /locus_tag="AC16CY_22525"
     CDS             833..1324
                     /locus_tag="AC16CY_22525"
                     /inference="COORDINATES: protein motif:HMM:NF046164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HIR3356645.1"
                     /translation="MPPKSAQLQVRVTPTQKAALKRLAHRAGLDVSSYVLSRALPDGP
                     ARFRAMLDEFRRGADRRFVLAEINDLLTELTPAPFSDAVADVDLRGLSPLLQNYVAAM
                     VEHAASRKGVTPPPWARDVEPMDEPYFAVPFPRLRPHLLRAAPVAFKRRNLFVDSSVG
                     DRV"
     gene            1335..1856
                     /locus_tag="AC16CY_22530"
     CDS             1335..1856
                     /locus_tag="AC16CY_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356646.1"
                     /translation="MPRLSKQDIHRLFELLDAELATEDVEGELYLVGGAVMCVALGAR
                     DATRDVDAFFKPSRVVREAATRVAAKAGVPDTWLNDAVKGYLSPRGEFELFLELPHLR
                     VFVAHPRYLLAMKCAAMRLGEEFHDVDDVRYLLRHLGVSKVDEALAIVSQYFDEKELL
                     PKTRLILEELLGQ"
     gene            complement(1878..2699)
                     /locus_tag="AC16CY_22535"
     CDS             complement(1878..2699)
                     /locus_tag="AC16CY_22535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3356647.1"
                     /translation="MRVAATAALVLVAAGRLSAQAPTIEPVRTETLAHPDDPSKRIEI
                     YWTKPAGEGPWPVLLLVHGHQEPERPGAKAYVDSGSFERYASRGLLVAAVSQPGYGKS
                     DGPPDFCGPRSQRAVIAAIDHFRKQPFVDAKRMALYGYSRGAVVASMVSSQVPDLAAV
                     VLGGGIYDLKATYTRMARGIQRNIDQEAGKSDEAYRARSAIFHVNRIKSPTIVLHGEQ
                     DDRASAESAKVFGAALEKTGTPVRVVIFPGVGHGIPPPQQTAEWAPFLRQYLGTK"
     gene            complement(2709..3440)
                     /locus_tag="AC16CY_22540"
     CDS             complement(2709..3440)
                     /locus_tag="AC16CY_22540"
                     /inference="COORDINATES: protein motif:HMM:NF013360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HIR3356648.1"
                     /translation="MRLSMRRRVVVIGFVFLCLTSAAPADAQRTSVGREFPVYDNGGR
                     PDLTMDPKRFVSQMEIVDRYFDPIDDACAFVEGVIGGAGYRRLLRFDTVVLNAGDADL
                     VVGDRSDPDNPYAPAFEFSPCHGHYHIKDFSEYSLLRPNGTVAAVGHKQGFCFEDSLK
                     YGDNRSSGFDCSFQGITSGWGDWYYKQLSGQWIDITGVPEGDYIVRATINMVKTFDEG
                     TDLYPDTIEVRIHVPDPRKKVTIVE"
     gene            complement(3472..>4397)
                     /locus_tag="AC16CY_22545"
     CDS             complement(3472..>4397)
                     /locus_tag="AC16CY_22545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356649.1"
                     /translation="DAKGQVRWDAPTPRTADGKPDLSGVWQRANSGPPRGRGGGGRQG
                     RGGGQGGGQAQPAAPPAAPAAPAAQPAAGEPGNTNTPFAGGRGGVQLEPPTEAFPFDP
                     AGPPVATFFEAGGNMEGGLPYTEWSRAIRKQRFDVDKAKDNPDANCMPMGFLQFHQQP
                     QPRMIVQTPKMILIEYEANYGLRHIYLDGRSLPPQGEPQPWWYGYSVGRWEGDTLVVE
                     TNNLRGAEDGPFDGWLDVNGSPHSQQAKFTERFRRLNYGRLQIDITLEDPKAYTKPFT
                     VRVDQRIVPEGELIEFICNENQQFRRRIKID"
BASE COUNT          717 a         1498 c         1424 g          758 t
ORIGIN      
        1 gagaatcctc cgagcacgat catcgacgca gtggccagcg gcaagatcga cgtcgccatc
       61 gtgtgggggc ccgccgccgg gtacttcgtg aagaaccagc gggttccgct ggcgatggtt
      121 cccgtgccgt ccgcgaaggg cgaccttccc ttcgagttcg acatcgccat gggcgtcaag
      181 atcgggaatg acgcgctcaa gacgcagctc caaaaagtgg tcgacgcgaa gcaagcggag
      241 attacgaaga tcctgaagga ctacggcgtg ccactgatcg acagaaaggc agactcgaaa
      301 cgatgaccac gcggcgcccg cacgggcagt tcccccctat cacgctgggc gcggccctgg
      361 cggttttcct gtcggtcctt gccggcgtcg tccacgagcg gctgccgctg cttcctgaca
      421 cgtcgcttgc cgcacaggcg ccggccgaca tctacaagga cgtctacgac ggctggaagt
      481 ggtggcacgt gtactgttat cgatgccacg gcacgaacgc gattgccacg acgctggcgc
      541 cggacctgat cgaccccaac cggcggctca ccgacgcgca gttcctcaag gtcgtgcgcg
      601 acggcgcgcc ggacaaaggc atgcaggcgt gggacaagct gctcgacaac aagcagattg
      661 gacagatcca cctgtacgtg cgcgcgcgca ccgacaaggt gctgccgccg ggacgacccg
      721 acgaggtcgg gccgaagggc ggcccatggg tgccgccggc cggctggccg aagcgctagt
      781 tgtcaatacg atagtattga cgatgaatgc acggcggtct atccttgcgg atatgccgcc
      841 caagtccgcg cagctgcagg tccgcgtcac acccacccag aaggccgccc tcaagcggct
      901 cgcccacaga gccggcctgg acgtctcgag ctacgtgctc tcccgcgcct tgccggacgg
      961 tcccgcccga ttccgcgcga tgttggacga gttccggcgc ggcgccgacc ggcggttcgt
     1021 gctcgctgag atcaacgacc tgctcaccga gctgactccg gctccgttct ctgacgccgt
     1081 cgctgatgtc gatctgcgag ggctctcccc cttgctccag aactacgtcg ccgcaatggt
     1141 cgagcacgcc gcgagccgta agggagtcac cccgccgccc tgggcacgcg acgtcgagcc
     1201 gatggacgag ccgtacttcg ccgtgccgtt cccccgactg cgtccacatc tgctgcgcgc
     1261 ggcgccggtc gcgttcaagc ggcggaatct gttcgtcgat tcgtccgtgg gcgacagggt
     1321 ctgagggacc cgccgtgccg cggctgtcga agcaggacat ccatcgtctg ttcgagctcc
     1381 tcgacgcgga gctcgcaacg gaagatgtcg agggcgagct ctatctggtg ggtggcgcag
     1441 tgatgtgcgt ggcccttggc gcgcgcgacg ccacacggga cgtcgacgcc ttcttcaagc
     1501 cgagccgggt cgtccgggaa gccgccacac gggtggccgc gaaggcgggc gtccccgaca
     1561 catggctcaa cgacgcggtc aagggctacc tgagtccacg cggcgagttc gagctcttcc
     1621 tcgaactccc ccatctccgg gtcttcgttg cgcacccccg ctatctgctg gcgatgaagt
     1681 gcgccgcgat gcgcctcggc gaggaattcc acgacgtgga tgacgtgcgc tacctcctcc
     1741 ggcacctcgg cgtctccaag gtcgatgagg cgctcgccat cgtcagccag tacttcgacg
     1801 aaaaggagct gctgccgaag acacgcctga tactcgagga actgctcggc caatagccga
     1861 ttccgagagc ccagagccta cttggtcccc aggtattggc gcaggaacgg cgcccactcg
     1921 gctgtctgct gcggcggcgg gatgccgtgg cccacacctg ggaagatgac gacccgaacc
     1981 ggagtcccgg tcttctccag agccgcgccg aagaccttcg ccgattcggc gctcgcgcga
     2041 tcgtcctgct cgccgtgcag gacgatcgtc ggcgacttga ttcggttcac atggaagatc
     2101 gccgagcgcg cgcggtacgc ctcgtcggac ttgccggcct cctgatcgat gttccgctga
     2161 atcccgcggg ccatcctcgt gtaggtggcc ttcagatcgt agatgccgcc gcccagcacg
     2221 acggccgcaa ggtcgggcac ctgcgaggac accatcgacg cgacgacggc gccgcggctg
     2281 tacccgtaca aggccattcg cttcgcatcg acgaacggct gcttccggaa gtgatcgatg
     2341 gcggcgatga ccgcgcgctg cgaacgaggc ccgcagaaat caggagggcc gtccgacttg
     2401 ccgtagccgg gttgtgacac ggcggctacc agaaggccgc ggctggcgta tcgctcgaaa
     2461 ctgccgctgt ctacgtacgc cttcgcgccc ggccgttcgg gctcctgatg cccgtgaacc
     2521 agaaggagga cgggccacgg cccctcgcca gctggtttcg tccagtagat ctcgatgcgc
     2581 ttcgacgggt cgtcggggtg ggccagtgtt tcggtccgga caggctcgat cgtcggtgcc
     2641 tgggcagaaa gccggcccgc cgctacgaga acgagcgccg ccgttgccgc aacgcgcatc
     2701 atcgcgtcct actcgacgat cgtgaccttc ttgcgcggat ccggcacgtg gatccgtact
     2761 tcgatcgtgt cgggatagag gtcggttcct tcatcgaagg tcttgaccat gttgatcgtg
     2821 gcccggacga tgtagtcgcc ttcgggcacc ccggtgatgt cgatccactg accagagagc
     2881 tgcttgtagt accaatcgcc ccacccgctc gtgatcccct ggaaactgca gtcgaacccg
     2941 ctgctccggt tgtccccgta cttgagagag tcctcgaagc agaagccctg cttgtgcccc
     3001 acggcggcca ctgtcccgtt ggggcgcagc agcgagtact cggagaagtc cttgatgtga
     3061 tagtggccgt ggcacggtga gaactcgaag gccggcgcgt acggattgtc gggatcggac
     3121 ctgtccccga caaccagatc ggcatcgccg gcgttgagga cgacggtgtc gaatctcagg
     3181 agacgccggt agcctgctcc accgatcacg ccctcgacga acgcacacgc atcgtcgatc
     3241 gggtcgaagt accgatcgac gatctccatc tgcgacacga accgcttcgg gtccatcgtc
     3301 aggtccggcc gtccgccgtt gtcgtacacg ggaaactcgc ggcccacgga cgtccgctgc
     3361 gcgtcggccg gcgccgcgct cgtcagacag agaaacacga agccaatcac gacgacgcga
     3421 cgccgcatgc tgagtctcat gacttcacac ccttcgaccg cccgagccgg gctagtcgat
     3481 cttgatccgt cgccggaact gctggttctc gttgcagatg aactcgatca gctcgccctc
     3541 cggcacgatc cgctgatcga cgcgaacggt gaacggcttc gtgtacgcct tcgggtcctc
     3601 gagggtgatg tcgatctgca ggcgcccgta gttcaatcgc cggaagcgct cggtgaattt
     3661 cgcctgctgg ctgtgcgggc tgccgttgac gtccagccac ccatcgaacg ggccgtcctc
     3721 cgcgccgcgc aggttgttgg tctcgacgac gagcgtgtcg ccctcccagc ggccgaccga
     3781 gtacccatac caccacggct gcggctcgcc ttgcggcggg agcgagcggc cgtccagata
     3841 gatgtggcgg aggccgtagt tcgcctcgta ctcgatgagg atcatctttg gcgtctggac
     3901 gatcatgcgc ggctgcggct gctggtgaaa ctgcaggaag cccatcggca tgcagttggc
     3961 gtcgggattg tccttcgcct tgtccacgtc gaaccgctgc ttccgtatcg cgcgcgacca
     4021 ctcggtgtac ggaaggccgc cctccatgtt gccgccggct tcgaagaacg tcgcgacggg
     4081 tggaccggcg ggatcgaacg gaaacgcttc ggtcggaggc tcgagctgca cgccaccgcg
     4141 tcctcccgcg aacggagtgt tcgtattgcc tggctcgccc gccgcgggct gcgcggctgg
     4201 tgctgccggc gccgctggcg gggccgcggg ctgcgcctgt ccgccgccct gtccaccgcc
     4261 gcgtccctgc cgtccgccgc cgccgcgtcc gcggggcgga ccgctgttgg cgcgctgcca
     4321 cacgccggaa agatcaggct tgccgtccgc cgtacgcggc gtcggcgcgt cccaccgcac
     4381 ctggcccttc gcgtcgc
//
LOCUS       DBNGSH010000986         2015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1836273, whole genome shotgun sequence.
ACCESSION   DBNGSH010000986 DBNGSH010000000
VERSION     DBNGSH010000986.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1836273"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(736..>2015)
                     /locus_tag="AC16CY_22550"
     CDS             complement(736..>2015)
                     /locus_tag="AC16CY_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR3356650.1"
                     /translation="ELSWAVMVYGFIASVLPVWLLLAPRDYLSAFMKVGTILVLGVAI
                     LVVMPPLRMPAVTQFVDGTGPVFAGKLFPFAFITIACGAVSGFHALVSSGTSPKMLRC
                     EPDARLVGYGSMLTESLVGVMAMVAAATLDPGVYFAMNVGPATLGTNSTTAAQAIAQW
                     GFTIGPQQLDALAREMGEATLLSRTGGAPSLAVGMAGILGNVFTGPGLALWYHFAIMF
                     EAVFILTTIDAGTRVGRFMLQELLGHVSAPLGRTSWYPSILLSSFIVVAGWGYFLYQG
                     VIDPLGGINSLWPLFGIANQLLAAVALCVATTVLAKMGKLRYTWVTLAPLAWLAAATL
                     TAGWQKVFSPDPKLGFLSHAQSLASAPGPNTARMIFNDRLDAGLALFFMAIVVVVILA
                     SAREWYLIASRRKAPAVNEAPFVESRLDVAAGL"
BASE COUNT          380 a          685 c          645 g          305 t
ORIGIN      
        1 ttcacggtgg gatccagccg aacgaggtgc acttcgccgc gtccaatcga gtcgcgccgt
       61 accgccaccg ccattccttg tcgtcgaatt ggctggatgt cggcgcgtca agaagtcggt
      121 cgtcacccct ggcgttcatg gatcttgctg catcggccca acctgcccgc gcggcccgag
      181 cagcgcgaac gattaagcgc ccatgttccg cctggagttc gacgtcgtcc ggcagctgcg
      241 cgtgctcaag caacattttg ggcacgcgaa tgcccctcga gtttccgatc tggactattc
      301 gggtacgggt gatcatgtga ctacagtgta atcacatcaa ccaccttcgg caaggaccgc
      361 actcttgcag gctaaccagt attgattgga ccgcccgatc gtctatcggc cctggcggct
      421 aatccaggtc agaacgcttc gttcgcgagg cggcaggcgg ttgaccgcgc ggatgacagc
      481 acgctgctac gaagacagcg ggcggagcga ggtttcctcg cgtctcttgt cggcctcggt
      541 gcgggaccgt aggtccgaag caaaaccgta gggagtgtac tcgcatacct cgttcgtggg
      601 ctgcgaagcc gcgatgcgtg gatcaagtcg gttttgcgga gcgacctacg gtcccgcgcc
      661 gcggccacga tgaccactcg gcgggtctgc cttcgacttc gctcaggcca ggaaagaccc
      721 gccgctacat ccagtctaaa gacccgccgc tacatccaga cgtgactcga caaacggcgc
      781 ctcgttcacg gcgggcgcct tccgtcgcga cgcgatcaga taccactcgc gcgcggacgc
      841 caggatcacg acaacgacga tcgccatgaa gaacagcgcg agccccgcgt cgagccgatc
      901 gttgaagatc atgcgcgcgg tgttcgggcc cggcgccgac gccagcgatt gcgcgtgcga
      961 gaggaacccg agcttcgggt ccggtgagaa caccttctgc cagcccgccg tcagcgtcgc
     1021 cgccgccagc catgcgagcg gcgccagcgt gacccaggtg tagcgaagct tccccatctt
     1081 tgccagcacc gtcgtcgcga cgcagagcgc gacggccgcc aggagctggt tggcgatccc
     1141 gaagaggggc cagagcgagt tgatgccccc gagcggatcg atcactccct ggtacaggaa
     1201 gtagccccac cccgcgacga cgatgaagga cgagaggaga atgctgggat accacgacgt
     1261 gcgcccgaga ggcgccgaga cgtgccccag cagctcctgc agcatgaagc ggccgacccg
     1321 cgtgcccgcg tcgatcgtcg tgagaatgaa gaccgcctcg aacatgatcg cgaagtggta
     1381 ccagagcgcg agacccgggc cggtgaacac gtttccgagg atccccgcca tgccgacagc
     1441 gaggctcggc gcgcctcccg tgcgcgacag gagcgtcgcc tcacccatct ccctggccag
     1501 cgcgtccagc tgttgcgggc cgatcgtgaa gccccactgc gcgatcgcct gcgccgccgt
     1561 cgtcgagttc gtgccgagcg tcgcgggacc gacgttcatc gcgaagtaga cgccgggatc
     1621 gagcgtcgcg gccgccacca tcgccatcac gccgacgagc gactcggtca gcatgctgcc
     1681 gtagccgacg agccgcgcgt cgggctcgca tctcagcatc ttcggagagg tgccgctcga
     1741 gaccagggcg tgaaacccgg acacggcccc gcacgcaatc gtgatgaacg cgaaggggaa
     1801 cagcttgccc gcgaacaccg ggccggtgcc gtccacgaac tgggtgaccg cgggcatccg
     1861 aagcggcggc atcaccacga ggatcgccac gccgagcacg aggatcgtcc cgaccttcat
     1921 gaacgccgac aggtagtccc ggggtgccag cagcagccac acggggagca ccgaggcgat
     1981 gaacccgtac accatcacgg cccaggacag ctcgg
//
LOCUS       DBNGSH010000987         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4214546, whole genome shotgun sequence.
ACCESSION   DBNGSH010000987 DBNGSH010000000
VERSION     DBNGSH010000987.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4214546"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="AC16CY_22555"
     CDS             complement(<1..638)
                     /locus_tag="AC16CY_22555"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3356651.1"
                     /translation="MLSVFALTLTALAQQNQPQIGIAPVTLNAAPYTFDTAELHKIRV
                     VIVARGLPHPFSLAFLPSGDALVTERGARLRIVRNATGAGGAQPALDPQPVAGIPATP
                     GFRGGGLQEVALHPGFAGNRFVYFTYNKAGAVGANPNQRQSAVTLARGRFDGKALTGV
                     EDLFTGDWSNGASGSRIAFGPNGHVFMTTGAPFGEQAQDSNTVYGKVLRLLD"
     gene            complement(694..1947)
                     /locus_tag="AC16CY_22560"
     CDS             complement(694..1947)
                     /locus_tag="AC16CY_22560"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3356652.1"
                     /translation="MKTASLCALFLTVVLTVPATTASTPTAKPEDVGLSSERLQRVTQ
                     MIDRHIAAGDLAGAVTIVARKGKVVHHSARGVMDLQSKQPMANGSMFRIASMTKPVVG
                     VAIMMLVEEGKVHLNDPIARYIPQFRDMRVAVARPAAGGRGAGAGAPQFYTVPADRAI
                     TVKDLLTHVSGLGSGAMGNSEIEKVARKEGETLADYIPRLGSTALEFQPGSRWTYSPG
                     AGFETLGRVIEVASGMKLDQFFRTRIFEPLGMTDITFWPTEAQWSRVASVYTRGEKGL
                     TKTVPPNDTMSRNVYFRGSGGLYSTAEDYIPLGMMLANGGELNGKRLLGRKTVEMFGA
                     VHVPDSLPGRPQGEGYGLSVRVVTDHAARGTMLSDGTFGWSGAQGTHFFVDPKEELVG
                     VLMVQTSIQEVQREFEDLVAQSIVD"
     gene            2045..>2830
                     /locus_tag="AC16CY_22565"
     CDS             2045..>2830
                     /locus_tag="AC16CY_22565"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR3356653.1"
                     /translation="MKYGVIPTTTIERLALLSGKVPVPMIDALFGPLKTRALMAAVRL
                     GVFEAMREGEHTPAALAATLHLDPDTLELLLRGLVVCDYVRQRPGNRFALSRLGRRSM
                     IDGAPMEFVGFMRFNYRQWEFIGHLEDLLRTGRGVDFHETMTDADSWRDYQRAMLERA
                     RFDAPIVASRVPVRKGATSLLDIAGSHGLFGAAICRRHPPMRSTVLDLPQAISHARAL
                     ASAEGIDDVVTHREGDLFASDLGSNHDVVLLLNILHHFTEDRIL"
BASE COUNT          440 a          969 c          939 g          482 t
ORIGIN      
        1 tcgtcgagca accgcaggac cttgccgtaa accgtgttcg agtcctgcgc ctgttcgccg
       61 aacggagcgc ccgtagtcat gaacacatgc ccgttcggac cgaaggcgat gcgcgacccg
      121 ctggcgccgt tgctccagtc gccggtgaac agatcctcga cgcccgtcag cgccttgccg
      181 tcgaatcgtc cgcgcgccag ggtgacggcg ctctgccgct ggttcgggtt cgcgcccacg
      241 gcgcccgcct tgttgtaagt gaagtacacg aacctgttgc cggcgaatcc cggatgcagc
      301 gcgacctcct gcaggcctcc gcccctgaac cctggggtcg cagggatgcc ggcgaccggc
      361 tgcggatcga gcgccggctg cgcgccgccg gcgccggtcg cgttgcgcac gatccggagg
      421 cgcgcaccgc gttcggtgac gagcgcgtcg cccgacggga gaaacgcgag gctgaaggga
      481 tgtggcaagc cacgcgcgac gatgacgacc ctgatcttgt gcagctcggc cgtgtcgaac
      541 gtgtacggcg ccgcgttgag cgtcacgggg gcgatgccga tctgtggttg attctgctgc
      601 gccagcgccg tgagcgtcag cgcgaagacc gagagcaccg tcaagaacga agaaacccgc
      661 cgaagagtgg acatcgcgtg taggctccct gtgctagtcg acgattgatt gcgcgacgag
      721 atcctcgaac tcgcgctgaa cttcctggat cgaggtctgc accatcagca cgccgaccag
      781 ttcctccttc gggtccacga agaagtgcgt gccctgggcg ccgctccatc caaacgtgcc
      841 atcggacagc atcgtgcctc tcgcggcgtg gtcggtgacg acgcggacgc tcaacccgta
      901 gccctctccc tgcggccgcc ccggcagcga atcggggacg tggacggcgc cgaacatctc
      961 gacggtcttg cggcccagga ggcgcttgcc gttcagttca ccaccgttgg ccagcatcat
     1021 gcccagcggg atgtagtcct cggccgtgct gtacaacccg ccggagccgc ggaagtagac
     1081 gtttctcgac atcgtgtcgt tcggtggcac ggtcttcgtc agacccttct cgccgcgcgt
     1141 gtacacgctc gccacgcgcg accactgtgc ctcggtcggc cagaaggtga tgtcggtcat
     1201 ccccagcggc tcgaagatgc gcgtgcggaa gaactgatcg agcttcatgc ctgatgcgac
     1261 ctcgatgacg cggccgagcg tttcgaagcc ggcgccgggg ctgtaggtcc accgcgatcc
     1321 cggctggaat tcgagcgcgg tcgagccgag gcgggggatg tagtcggcca gcgtctcgcc
     1381 ttccttgcgc gcgacctttt cgatctcgct gttgcccatc gccccgctcc cgaggcccga
     1441 gacgtgcgtc agcaggtctt tgaccgtgat cgcgcgatcg gcgggaaccg tgtagaactg
     1501 cggagcgccg gcgcccgcgc cgcgcccgcc cgcggccgga cgcgccacgg ccactctcat
     1561 gtcgcggaac tgcgggatgt agcgggcgat cggatcgttg aggtgcacct tgccctcctc
     1621 gaccagcatc atgatcgcca cgcccacgac gggcttcgtc atcgaggcga tgcggaacat
     1681 cgagccgttc gccatcggct gcttcgactg caggtccatc actccgcgcg cggaatggtg
     1741 cacgaccttc cccttccgcg ccacgatcgt gacggcgccc gcgaggtcgc ccgcggcgat
     1801 gtggcgatcg atcatctggg tcacgcgctg gagccgctcc gacgagagcc ccacgtcctc
     1861 gggctttgcc gtgggagtgg aggcggtcgt cgccggcacg gtcaggacga cggtgaggaa
     1921 gagggcgcac agggaagcag tcttcatggg aggagtctcc tgagtacgta gtgtccggct
     1981 tcagccggac cttttcatgc tgacgacttg cgcagattat gccccgccgc gccatagtta
     2041 ggccatgaaa tacggtgtga tcccgaccac gacgatcgag cggctcgcgc tgctgtcagg
     2101 caaggtcccc gtgccgatga tcgacgcgct cttcggcccc ctgaaaacgc gtgccctcat
     2161 ggcggcggtc aggctcggcg tattcgaggc gatgcgcgag ggcgagcaca ccccggccgc
     2221 tctcgccgcg acgctgcacc tggatcccga cacgctcgag ctgttgctgc gcggcctcgt
     2281 cgtctgcgac tacgtgcgtc agcgccccgg aaaccgtttc gcgctctcca ggctcgggcg
     2341 ccgctcgatg atcgacggcg cgccgatgga gttcgtcggg ttcatgcgct tcaactaccg
     2401 gcagtgggag ttcatcgggc acctcgagga tctcctgcga acgggccgcg gcgtcgactt
     2461 ccacgagacg atgaccgatg cggacagctg gcgcgactac cagcgggcca tgctggagcg
     2521 cgcccggttc gacgcgccga tcgtcgcctc gcgcgtcccg gtacggaaag gggcgacgag
     2581 cctgctggac attgccggct cgcacggcct cttcggcgcc gcgatctgcc ggcggcatcc
     2641 gccgatgcgc tccacggtgc tcgaccttcc gcaggccatc tcccacgcac gcgcgctcgc
     2701 cagcgccgaa gggatcgacg acgtggtgac gcaccgcgaa ggggacctgt tcgcctcgga
     2761 tctggggtcg aatcacgacg tcgtgctgct gctcaacatc ctccatcact tcacggagga
     2821 ccggattctg
//
LOCUS       DBNGSH010000988         6021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2348028, whole genome shotgun sequence.
ACCESSION   DBNGSH010000988 DBNGSH010000000
VERSION     DBNGSH010000988.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2348028"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /gene="rsfS"
                     /locus_tag="AC16CY_22570"
     CDS             <1..318
                     /gene="rsfS"
                     /locus_tag="AC16CY_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR3356654.1"
                     /translation="VRDKKAEDVVVLDLRKTGAFTDFFVICTGGNPRQMTAIADSVRD
                     ALKQTLGERPTLAEGIDRSEWILLDYFNFVVHIFSRERRVFYGLERLWGNAARHEFGN
                     EIK"
     gene            327..1112
                     /locus_tag="AC16CY_22575"
     CDS             327..1112
                     /locus_tag="AC16CY_22575"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR3356655.1"
                     /translation="MFLRDPALSAADALLAALFAPCCAACSNPLERPTRGPVCDACWA
                     AVALVRPPFCRTCGSMLASWRTLDGAAGLCPLCRRRPGAVDAARSVGPYEGALRDIVH
                     AFKYEARRTLAPALGGMMREAGCDLLAGAACAVPVPLHPWRRFRRGFNQSADLARSLG
                     LPVVHALWRMRATAPQTGLPAAGRRRNVRGAFSASPLLSHRVHRTLIVNRAVVLVDDV
                     RTTGATLDACARALKAAGAAEVRTLTVALADHPARRRRSFHAM"
     gene            complement(1073..1477)
                     /locus_tag="AC16CY_22580"
     CDS             complement(1073..1477)
                     /locus_tag="AC16CY_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356656.1"
                     /translation="MNTIDRLAGSFTGSGAWHDEVGKSAAYTVQHTNRETGTGFDVAF
                     KHDFEDGTVVDARFGMTWIAPGIFRVDVAGTPVGNGYVFEGYCHYHLKLGETFVEASY
                     RSTDSGLEVFGSSTANAEGHYIAWKERLRRAG"
     gene            complement(1492..2079)
                     /gene="tmk"
                     /locus_tag="AC16CY_22585"
     CDS             complement(1492..2079)
                     /gene="tmk"
                     /locus_tag="AC16CY_22585"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3356657.1"
                     /translation="MRGLLIAFEGLDQSGKQTQAERVRDRLVGSGRVVELLSFPAYHT
                     PIGFEIRQALEGTRRWRADVMQLLYIANRYEAKPRIEDALARGTIVVCDRYVASSVAY
                     GESQGLDAEWLHDIQRYLPQPDLTILLDIAPEVSAQRKTAGRDAYERDLALLARVRVS
                     YLSQARARGWLLLDASRDREKIASEIFEALEPLLR"
     gene            complement(2105..3358)
                     /gene="lysA"
                     /locus_tag="AC16CY_22590"
     CDS             complement(2105..3358)
                     /gene="lysA"
                     /locus_tag="AC16CY_22590"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR3356658.1"
                     /translation="MRRLVYLPFQPSAAGLHCDQVSVASIVERVGTPVYVYSARAVRE
                     SYREIDDAFAGYPHAIHYALKANSTLALVRLLRSLGSRADANSGGEIHVALRAGFEPR
                     DIVFTGVGKTREELDDAVARDIGTINAESPGELDRIAALAAARGRVARVALRVNPDID
                     ARSHPSISTGLKSNKFGVAIEEARAICAERKGVRSLRFVGVHVHIGSQITAAEPLARA
                     ARALVSLALELAADGFALEHVDLGGGLGIGYDGNAALAPAGYAAAVLPELRRIGIPVI
                     LEPGRAIVGHAGALVSRVVDVKRYPDGRQFAVLDAAMSELLRPALYGSYHRIVPVAPR
                     GGESPWDIVGPICESSDVFARERLLPDLCVDDLVAVLDTGAYGAVMASNYNRRFLAPE
                     VLVDGPQWSVIRRRQTLDDVLALET"
     gene            3365..4087
                     /locus_tag="AC16CY_22595"
     CDS             3365..4087
                     /locus_tag="AC16CY_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3356659.1"
                     /translation="MRASSASRVRARFICPIATCVAIPRFAREPTRSLLPEMPDAPST
                     TFATDEVIERCLKGDQAAWELIVRSYRRKVFNVAYKFVGRHDQAEDLTQDVFLKLYKS
                     LDTFDRRANFQTWLISVSRNLCIDHYRAVRKERETINRDVDPADFAPSAPEARADVQL
                     EQRDRVKLLRQALDKLAPTLRTAVMLRDIQELTYQEIADRLRLPEGTVKSRINRGRTE
                     LARQIQRLREEQDQVPRAGVSG"
     gene            4084..4425
                     /locus_tag="AC16CY_22600"
     CDS             4084..4425
                     /locus_tag="AC16CY_22600"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3356660.1"
                     /translation="MNLTTDRNQNVAVVRVNETRLMYPILSDFASTITSLITGGEKRL
                     LLDLSAVTYVDSATIGCLMDLYRQASAAGGALKLSGVQKRVETMLSMTGAQNFLEIHP
                     DEASAIRSFGV"
     gene            4422..4691
                     /locus_tag="AC16CY_22605"
     CDS             4422..4691
                     /locus_tag="AC16CY_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356661.1"
                     /translation="MSTRTIKTTTGAEIPLDGDLLEVMEILYREVTARRELERSFEDM
                     AREIGHLIAQMDDAERRAYLLESLFLNTVTYENDKLEAYMRKIAK"
     gene            4849..5520
                     /locus_tag="AC16CY_22610"
     CDS             4849..5520
                     /locus_tag="AC16CY_22610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3356662.1"
                     /translation="MTRIRYCPRVVVALFSVLALLAAHQLEAQTQFRVQPTPRLTFEA
                     ASIKERDPKAPLGLVGLEVLPGRLVGRCVSLKALLFYAYHLTLSSPITGLQDWADTSC
                     NYSSGTNTYELQATMPSETTDAQAREMMQSLLAERFKLAVHWDTKTRPVFALVIGSVG
                     FKLKPSDPKDDPARALSSIGCPPDDRACRMIVMGSGPTSALARSSGVCRMAASPSRRR
                     DQRRG"
     gene            complement(5735..>6021)
                     /locus_tag="AC16CY_22615"
     CDS             complement(5735..>6021)
                     /locus_tag="AC16CY_22615"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR3356663.1"
                     /translation="NAIVISDTGVLNNGLRFDDEFVRHKILDVIGDMALVGHPMIGHL
                     VAHRGGHALHTAFAAKVLEEGDSWTLESGAADAAADVPAPIPATGAARAS"
BASE COUNT         1041 a         2010 c         1966 g         1004 t
ORIGIN      
        1 gtccgcgaca agaaggcgga ggacgtcgtc gtgctcgatc tgcgcaagac cggcgccttc
       61 accgacttct tcgtcatctg cacgggaggc aatccccggc agatgacggc gatcgcggac
      121 tcggtgcggg acgcgctcaa gcaaacactc ggcgagcgcc cgaccctggc cgaagggatc
      181 gacaggtcgg aatggattct gctcgactac ttcaacttcg tcgtccacat cttcagcagg
      241 gaacgccgcg tcttctacgg cctcgagcgg ctctggggca acgccgcacg ccacgaattc
      301 gggaacgaga ttaagtagcc gtagccgtgt ttcttcgtga ccctgcgctc tccgccgccg
      361 atgcgctgct cgcggcgctg tttgccccct gctgcgccgc ctgctcaaat ccgctcgaga
      421 ggccgacgag gggaccagtg tgtgacgcat gctgggcggc cgtcgccctc gtcagaccgc
      481 ctttctgccg aacctgcgga agcatgctgg cgtcgtggag gacgctcgac ggggccgcgg
      541 gcctgtgccc cctctgccgc cggcggcccg gggccgtcga tgccgcacga tcggtcggtc
      601 cctacgaagg tgctctccgc gacatcgtcc acgcgttcaa gtacgaggcg cggcggacgc
      661 tcgcaccggc actgggcggc atgatgcgcg aggccggatg cgatctcctc gcgggcgccg
      721 cgtgcgccgt gcccgttcct cttcatccgt ggcgccgctt caggagaggc ttcaaccagt
      781 cggccgatct cgcacgctcg ctcggcctgc ccgtagtcca tgcgttgtgg cgaatgcggg
      841 cgacggctcc gcaaaccggg ctcccggccg cggggcggcg ccgcaacgtg agaggagcgt
      901 tctcggcttc cccgctcctc tcgcaccgcg tgcaccgaac gctgattgtc aatcgcgccg
      961 tcgtgctcgt tgacgatgtg aggacgacgg gggcaacgct cgacgcgtgc gcgcgagcgc
     1021 tgaaggcggc cggcgcggcg gaagtgcgca cgctcaccgt ggcgctggcg gatcaccccg
     1081 cgcggcggag acgctccttc cacgcgatgt aatgaccctc ggcgttcgcc gtgctcgagc
     1141 cgaacacttc cagccccgag tccgtcgaac ggtaactcgc ctcgacgaac gtctcaccaa
     1201 gtttcaggtg gtagtggcag taaccctcga acacgtatcc gttgccgacc ggtgtcccgg
     1261 cgacgtccac gcggaagatg ccgggggcga tccaggtcat gccgaagcgg gcatcgacga
     1321 ccgtgccgtc ctcgaagtcg tgcttgaagg cgacgtcgaa gccggtcccc gtttcccggt
     1381 tggtgtgctg gaccgtgtac gccgccgact tgccgacctc gtcgtgccac gctccagacc
     1441 ccgtgaacga tccggcaagt cggtcgatgg tgttcatcgg cctcctccac ctcagcgaag
     1501 caacggctcc agcgcttcga atatctccga cgcgatcttc tcgcgatcgc gactggcgtc
     1561 gaggagcagc cagccgcgcg cccgcgcctg actcaggtag ctcactcgca cgcgggcgag
     1621 cagcgcgaga tcgcgttcgt aggcgtctcg gccagcggtc ttgcgctgcg cggagacctc
     1681 gggcgcgatg tcgagcagaa tcgtgagatc cggctgtggc aggtagcgct ggatgtcgtg
     1741 cagccattcg gcgtccagcc cctgcgattc cccgtacgcg acgctcgagg cgacgtagcg
     1801 gtcgcagacg acgatcgtgc cgcgggcaag cgcatcttcg atccgcggct tcgcttcgta
     1861 gcggttggca atgtagagaa gctgcatcac gtcggcacgc cagcggcggg tcccttcgag
     1921 ggcctgccgg atctcgaagc cgattggcgt gtggtacgcg gggaacgaca gcagttccac
     1981 gacgcggccc gagccgacca gccgatcccg tacgcgctcg gcctgagtct gcttgccgct
     2041 ctggtcgaga ccttcaaatg cgatcagcag gccgcgcata cgccgttcgg taccatgaga
     2101 ggcatcacgt ctcgagcgcg aggacgtcgt cgagcgtctg cctccgccgg atgacgctcc
     2161 attgcgggcc gtccacgagt acttccgggg ccagaaagcg ccggttgtag ttcgacgcca
     2221 tgaccgcgcc gtacgcgccg gtatcgagca cggcgacgag atcgtccacg cagaggtctg
     2281 gcagcagccg ctcgcgcgcg aagacgtcgc tgctctcgca gatcggtccc acgatgtccc
     2341 acgggctctc gccgccgcgc ggcgccacgg gcacgatgcg gtgataggag ccgtagagag
     2401 cggggcgcag cagctccgac atcgccgcgt cgagcacggc gaactgacgg ccgtcggggt
     2461 agcgcttgac gtcgacgacg cgcgacacca gggcgccggc gtgcccgacg atcgcgcgcc
     2521 cgggttcgag gatcacggga atcccgatcc gccggagctc cggcagcacc gcggcggcgt
     2581 atccggcggg agccagggcc gcgttcccgt cgtacccgat acccaggccg cctccgaggt
     2641 ccacgtgttc gagcgcgaag ccgtcggcgg caagctcgag ggccagcgag accagcgctc
     2701 gcgccgcgcg cgccagcggc tcggcggccg tgatttggga tccgatgtgc acgtggacgc
     2761 cgacgaacct gaggctccgc acgcctttcc gctcggcgca gatggcgcgc gcctcctcga
     2821 tcgccacgcc gaacttgttg ctcttgaggc cggtggagat gctgggatga ctgcgtgcgt
     2881 cgatgtcggg gttgacgcgg agcgccacgc gcgcgacacg gccccgcgcc gccgccagcg
     2941 ccgcaatccg atccagctcg cctggcgatt ccgcgttgat cgtcccgatg tccctcgcca
     3001 ccgcgtcatc gagctcctcg cgcgtcttgc cgacgccggt gaacacgatg tcgcgcggct
     3061 cgaaaccggc gcggagcgcg acgtgaatct cgcctccgga gttggcgtcg gcgcggctcc
     3121 cgagcgagcg cagcagccgc acgagcgcca gcgtggagtt cgccttcagc gcgtagtgga
     3181 tggcgtgcgg gtacccggcg aacgcgtcgt cgatctcgcg gtacgactcg cgcaccgcgc
     3241 gggcgctgta cacgtacacg ggtgtgccga cccgctcgac gatggatgct acgctgacct
     3301 ggtcgcagtg aaggcccgct gccgagggct gaaacggtag gtacacgagc cttcgcatgt
     3361 tatcatgcgc gcatcttctg catcccgggt tcgagcgcgg ttcatctgtc cgatcgcgac
     3421 gtgcgtcgcc atcccgcgtt tcgcgcgcga gccgacgcgg agtctcctgc cagagatgcc
     3481 tgacgcgcct tcaacgacat ttgcaaccga cgaggtgatc gagcggtgcc tcaagggtga
     3541 tcaggccgcg tgggagctga tcgtacgctc gtaccgccgc aaggtcttca acgtcgcgta
     3601 caagttcgtc gggcgccacg atcaagccga ggatctcacg caggacgtgt ttctgaagct
     3661 gtacaagtcg ctcgacacgt tcgatcgccg cgccaacttt cagacgtggc tgatcagcgt
     3721 cagccgcaac ctgtgcatcg atcactaccg cgccgtccgc aaggagcgcg agacgatcaa
     3781 ccgcgacgtc gatccggcgg acttcgcccc ttccgccccc gaggcgcgcg ccgacgtgca
     3841 actggagcag cgagaccgcg tcaagctgct gcgccaggcg ctcgacaagc tggcgccgac
     3901 ccttcgcacg gccgtcatgc tgcgtgacat ccaggagctc acctaccagg agatcgccga
     3961 ccgcctgcgc ctgccggagg ggaccgtcaa atctcgcatc aatcggggac ggactgaact
     4021 cgcgcgccag attcaacgtc tgcgtgaaga gcaggatcag gtcccgagag ccggagtatc
     4081 aggatgaatc tgaccaccga ccgtaaccag aacgtggccg tcgtccgggt caacgagacc
     4141 cggctgatgt acccgatcct gtccgatttc gcgagtacca tcacgtcgct catcaccggc
     4201 ggcgaaaagc ggctgctgct cgacctctcc gcggtgacct acgtggacag cgcgacgatc
     4261 ggctgcctga tggatctgta ccgccaggcg agcgccgcgg gcggagcgct gaaattgtcg
     4321 ggcgtgcaga aacgcgtcga aaccatgctc tcgatgacgg gagcccagaa cttcctcgag
     4381 attcatcccg acgaggcgtc ggctatcagg agcttcgggg tatgagtacg cgaaccatca
     4441 agacgacgac cggcgccgag atcccgctcg acggcgacct gctggaggtc atggaaatcc
     4501 tgtaccggga agtcacggcc aggcgcgaac tggaacgctc gttcgaggac atggcgcgcg
     4561 agatcggcca cctgatcgcg cagatggacg acgccgagcg ccgggcctac ctgctggaga
     4621 gcctgttcct caacaccgtt acctacgaga acgacaagct cgaagcctat atgaggaaga
     4681 tcgcgaagta gggggcaggg gttaggggtc agttggcgct cgcgggctga tcctttcgtg
     4741 acgagcaccg gcgattggcg ccttccacct acacaacgtt aggcaacaat cccgagtcca
     4801 gcaaggtgta gaacaggacg tacactcacg ccacatcagg gtgagaacat gacgcggatt
     4861 cgctattgcc ctcgagtcgt cgtcgcgttg ttttcagttc tggccctgtt ggcagctcac
     4921 caactggagg cacaaacgca gtttcgcgtg caaccgacgc ctcggctcac cttcgaagcc
     4981 gcatcaatca aggaacgcga cccgaaggca ccactcgggc tggtgggact agaagtcttg
     5041 ccaggcaggc tcgttggtcg ttgcgtcagt ctcaaggctc tcttgttcta cgcctatcac
     5101 ctcacactct catcaccaat caccggtctt caggattggg ccgacacgtc ctgcaactac
     5161 tccagcggca cgaacacgta tgaactccag gcgacaatgc cgtccgagac caccgacgcg
     5221 caagccaggg agatgatgca gagcctgctc gcagagcgct tcaagctggc cgtgcactgg
     5281 gacacgaaga cgaggccggt cttcgcgctt gttattggat cggttggttt caagctcaag
     5341 ccgtccgatc ccaaggatga tccggcaaga gcactcagct cgatcggttg tccccccgac
     5401 gatcgcgcgt gtcgcatgat cgtcatgggt tcaggtccaa cttcggcgct tgcccggagc
     5461 tcaggtgtgt gtcgaatggc ggcaagccct tcgaggcgac gcgatcagcg aagaggataa
     5521 gaacgcgctg gaggctcgcc gggcggcgac agcgttgcca tcagcaaggt aaacgggtgc
     5581 caacgagagt gccggaggcg cgttctttcc gcgtcgaact cgcgggcttg ccgacgttcc
     5641 acacacacct gagcgacgaa tagtgattcg aggcagtggc gggaagggcg actgtgagct
     5701 atccgctctg aaccgtgaac cgtagactag agtgctagct ggcgcgcgcc gcgcccgtgg
     5761 cgggaatcgg cgcaggcacg tcggccgccg cgtcagcagc ccccgattcg agcgtccacg
     5821 agtcgccctc ctcgagaacc tttgccgcaa acgccgtgtg cagcgcgtgg cctccgcgat
     5881 gcgcgacgag gtgcccgatc atcgggtgcc cgacgagcgc catgtcgcca atgacgtcca
     5941 ggatcttgtg ccgcacgaac tcgtcgtcga accgcaggcc gttgttcagc acgcccgtgt
     6001 cggagatgac gatggcgttc t
//
LOCUS       DBNGSH010000989         2489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3026130, whole genome shotgun sequence.
ACCESSION   DBNGSH010000989 DBNGSH010000000
VERSION     DBNGSH010000989.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3026130"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="AC16CY_22620"
     CDS             <1..466
                     /locus_tag="AC16CY_22620"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3356664.1"
                     /translation="SVEPEIGSVAQYTDLVRGSGGLYRLGFGRPSLCGSLDDLRQHGS
                     DPTEAAFNKGVLCSPTTAAGGYPNNLGYGNLGRNELRGFWQRRVDLSLAKAFALGGAR
                     NIELRWDIFNVFNTVNYALPNNVIGAATTDFGKITDSVGGPRVMQVGARFRF"
     gene            complement(601..1443)
                     /locus_tag="AC16CY_22625"
     CDS             complement(601..1443)
                     /locus_tag="AC16CY_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR3356665.1"
                     /translation="MNAIKDAVQEKYGEAAQQARLGKKAGCGCGPACCETITSDLYDG
                     AEAAAVPAEAMLASLGCGNPTALAELKPGEVVLDLGSGGGIDVLLSAKRVGPTGKAYG
                     VDMTDEMLALARENQVKSGLTNVEFLKGEIEHIPLPDGSVDVIISNCVINLSADKDRV
                     IAEAFRVLKPGGRFAVSDVVVRGEVPEAVRRSMELWTGCVAGALEEGSYRRKLTEAGF
                     ENVDVEPTRIYSGADAEALLTEGGLSDETIAADIDGRFMSAFVRAQKPTAVRNCCASS
                     CCSS"
     gene            1511..1924
                     /locus_tag="AC16CY_22630"
     CDS             1511..1924
                     /locus_tag="AC16CY_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3356666.1"
                     /translation="MKRPSAARNRAAIDQVEEVFKALADKTRLRILALLGRNEVCVCH
                     IHDSLGLPQPTVSRHLAYLRRSGLVAVRRDGVWMHYRVSQSLDPVILGVVSVAVEALK
                     HVPAAAEDRRQFQRSFGDLYVLNSPAGGTCCAPRA"
     gene            1943..2374
                     /gene="arsN2"
                     /locus_tag="AC16CY_22635"
     CDS             1943..2374
                     /gene="arsN2"
                     /locus_tag="AC16CY_22635"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HIR3356667.1"
                     /translation="MIEAAIEGDLPAVRDLLARLQLPLDEIDDHVQTMIVAREEGQVV
                     GTAALEVYADGALLRSVAVNPARQGRRLGHQLTEAALRLARTRGADTVFLLTTTAERF
                     FPRFGFEPIARDEVPASVLASVEFRSACCASAIVMRKRLGP"
     gene            2371..>2489
                     /locus_tag="AC16CY_22640"
     CDS             2371..>2489
                     /locus_tag="AC16CY_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase/protein-tyrosine-phosphatase
                     family protein"
                     /protein_id="HIR3356668.1"
                     /translation="MTTVLFACVHNAGRSQMAAAWFNLRADPAKARAISAGTDP"
BASE COUNT          404 a          847 c          805 g          433 t
ORIGIN      
        1 ctcggtcgag cccgagatcg gaagcgtggc ccagtacacc gacctggttc ggggttcggg
       61 cggcctctac cgcctcgggt tcgggcggcc gagcctgtgc ggatcgctgg acgatcttcg
      121 ccagcacggc agcgacccca cggaggccgc attcaacaag ggcgtcctct gctcgccgac
      181 gacggcggcg gggggatatc ccaacaacct gggatacggc aacctgggcc gcaacgagct
      241 ccgcgggttc tggcaacggc gcgtggacct gagcctggcg aaggcgttcg cgcttggcgg
      301 cgcgcggaac atcgagctcc gctgggacat cttcaacgtg ttcaacacgg tcaactacgc
      361 gctgcccaac aacgtgatcg gcgcggccac caccgacttt ggcaagatca ccgactcggt
      421 gggcggcccg cgcgtgatgc aggtcggcgc ccggttccgg ttctgagagc ctgagacgaa
      481 atcacgtcca acgacagccg ccacgaagag ctcggccacg aagtttcacg aagccatttg
      541 aatcttcgtg tccttcgtgg cagacggtct tcgtgatcct cgtggcccga gttcccggga
      601 tcaggaagag cagcagctcg acgcacagca gttccgcacg gctgtcggct tctgcgcccg
      661 gacgaaggcg ctcatgaagc gcccatcgat gtccgccgcg atcgtctcgt ccgacaatcc
      721 gccttccgtc agaagcgcct cggcgtctgc gccgctgtag atacgcgtcg gctcgacgtc
      781 cacgttctcg aatcctgcct ccgtcagttt gcgccggtag ctgccctctt cgagggcgcc
      841 cgccacgcac ccggtccaca actccatgct gcgtcgaacc gcctcgggca cctcgcccct
      901 gacgaccaca tccgacactg cgaagcgccc cccgggcttc agcacgcgga acgcctcggc
      961 gatcacccgg tccttgtccg ccgagagatt gatgacgcag ttggagatga tgacgtcgac
     1021 cgagccgtca gggagcggaa tgtgctcgat ctcgcccttc aggaactcga cgttcgtgag
     1081 ccccgacttc acctggttct cacgggcgag cgccagcatc tcgtccgtca tgtccacgcc
     1141 gtacgccttg ccggtgggac cgacccgctt cgccgacagc aggacgtcga tgcccccgcc
     1201 cgagccgaga tcgagcacca cctcgcccgg cttcagttcg gcgagcgcag tcggattgcc
     1261 gcacccgagg gatgccagca tcgcctccgc cgggacggcc gcggcctccg cgccgtcgta
     1321 gagatccgag gtgatcgtct cgcagcaggc gggcccgcat ccgcatcctg ctttcttccc
     1381 gagacgagcc tgctgcgccg cctcgccgta tttctcctgg accgcgtctt tgatcgcatt
     1441 catgggtgtc tcctaaatat tcgcatttgc gaatatatag ggaccgtggt atattcgtca
     1501 agacaaatat atgaagcgac cttcggccgc ccggaatcgg gccgcgatcg accaggtgga
     1561 agaggtcttc aaggccctgg ccgacaagac ccgtcttcga atccttgcct tgctcggcag
     1621 aaacgaagtg tgcgtctgtc acattcacga cagcctgggg ctgccgcagc cgacggtgtc
     1681 ccggcacctg gcgtacctgc ggcgcagcgg gctggttgcc gtcaggcgcg acggcgtgtg
     1741 gatgcactat cgggtgtcgc agtcgctcga ccccgtgatc ctcggtgtcg tcagcgtcgc
     1801 cgtcgaggcg ttgaagcacg tccccgccgc agccgaggac cgcaggcagt ttcagcgatc
     1861 gttcggagat ctctatgtgc tgaacagccc cgccggaggg acgtgctgcg cgccgcgcgc
     1921 gtagaggagt agacgagacg agatgatcga agctgcgatc gagggcgatc tcccggcggt
     1981 gcgcgacctc ctcgcgcgcc tccagctgcc gctggatgaa atcgacgacc acgtccagac
     2041 gatgatcgtg gcccgggagg aggggcaggt ggtcggcacg gcggccctcg aggtgtatgc
     2101 cgatggcgcc cttctgcgat cggtcgccgt caatccggcc cggcaggggc gcaggctcgg
     2161 gcatcagctg accgaggcgg cgctgcgcct tgcccggacg cgcggcgccg acacggtgtt
     2221 tctcctgacg accacggctg aacggttctt cccgagattc ggcttcgagc cgatcgcgcg
     2281 tgacgaggtg ccggcatcgg tgctggcctc ggtcgagttc cgctccgcct gctgcgcgtc
     2341 agcgatcgtg atgcgcaagc ggctcggacc atgaccaccg ttctcttcgc gtgcgttcac
     2401 aacgcaggac ggtcgcagat ggcggccgcc tggttcaatc tgcgtgcgga cccggcgaag
     2461 gcgagggcga tctcggcggg gaccgatcc
//
LOCUS       DBNGSH010000990         1978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_310704, whole genome shotgun sequence.
ACCESSION   DBNGSH010000990 DBNGSH010000000
VERSION     DBNGSH010000990.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_310704"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1334)
                     /locus_tag="AC16CY_22645"
     CDS             complement(177..1334)
                     /locus_tag="AC16CY_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HIR3356669.1"
                     /translation="MTQLGGVRSKLPAGGVSIFAVMTRLSNEVGAINLSQGFPDFDTS
                     PELVESVARQMRDGHNQYAPMPGVLALREALSLKIERLYGRRYDPATEITITAGATEG
                     LFASITALVHPGDEVLLFQPAYDSYTPAVLLSGGVPVYATLRYPDYRIDWDEVRRKVS
                     SRTRAIVINSPHNPTGMVWTSGDVRELGRILQGTDAVVIADEVYEHILFDGTRHESLA
                     RYPEIASRAVVISSFAKTYHTTGWKVGYCAAPASITSEIQGVHQWITFAVNTPIQMAY
                     ADLVRRNPECRDVTVFYQNRRDTFLSLIQGSRFRPLAGRGTFFQMLDYSDITKEGDAD
                     FAIRLTRDHGVAAIPISPFLYGNEPSGPVLRFCFAKRDATLERAAERLRGV"
     gene            complement(1354..1602)
                     /locus_tag="AC16CY_22650"
     CDS             complement(1354..1602)
                     /locus_tag="AC16CY_22650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356670.1"
                     /translation="MSVRRFRSDVLEQLWQHGVQPTPTTKPELVHEFVSDLYRYELRR
                     LRDRLVRKEIPKTGYFDRVVALRRRYALLSLKPHEWLE"
     gene            complement(1599..>1978)
                     /locus_tag="AC16CY_22655"
     CDS             complement(1599..>1978)
                     /locus_tag="AC16CY_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356671.1"
                     /translation="REEMALRLDHALRLETQLRAGGAPGAPIVSAHEAAGDLWLRVHR
                     FEDARRAYVNAAGEVGLTSRVHLGLARTAARLEDVNAACAEYRMLLGEWKLPETESAE
                     IAEARTFLGRPACQNPPARPSRP"
BASE COUNT          316 a          659 c          687 g          316 t
ORIGIN      
        1 ggcgcgctgg aagcgtccaa tgccgcgccc gtggccatgc tggggctggg aggcggcggc
       61 gtgctggcgt tcgtccgccg cgtcaaaggt ctcgtgtggg cggcgatcgg cctggcgtgc
      121 tacccgagag tcacgccgcg cgccccggcg ccatcgcccg cccgatagcg agcgagctag
      181 acgccgcgca gccgttcggc cgcgcgctcg agcgtggcgt cccgcttggc gaagcagaac
      241 cgcagcaccg gcccagacgg ttcgttcccg tagaggaacg gcgagatcgg aatggcggcc
      301 acgccgtgat cgcgcgtcag ccgtatcgcg aagtccgcat cgccttcctt cgtgatgtcc
      361 gagtagtcga gcatctggaa gaacgtgccg cggccggcca gcgggcggaa gcgcgagccc
      421 tgaatcagcg acagaaacgt gtcgcgcctg ttctggtaga acaccgtgac gtcgcgacac
      481 tcggggttgc gccggacgag atcggcgtag gccatctgga tcggggtgtt gacggcaaac
      541 gtgatccact ggtgcacgcc ctggatctcg gacgtgatcg aggccggcgc cgcacagtac
      601 ccgaccttcc agcccgtcgt gtggtaggtc ttggcgaacg agctgatcac caccgcccgg
      661 ctcgcgatct cgggatacct cgccaggctc tcgtgacgcg tgccgtcgaa caggatgtgc
      721 tcgtagacct cgtcggcgat caccacggcg tcggtgcctt gcaggatccg cccgagctcg
      781 cggacgtcgc cagacgtcca gaccatgccc gtcggattgt gcggcgagtt gatcacgatc
      841 gcgcgcgttc tcgaggacac cttgcgtcgc acctcgtccc agtcgatccg gtagtccgga
      901 taccgcagcg tggcgtacac gggcacgccg ccgctcaaca ggaccgcggg cgtataggaa
      961 tcgtacgccg gctggaacag gagtacttcg tcgcccgggt ggaccagcgc cgtgatcgag
     1021 gcgaacagcc cctccgtcgc gccggcggtg atcgtgattt cggttgcggg atcgtagcgc
     1081 cggccgtaca gccgctcgat cttgagggag agcgcttcgc gcagcgccag tacccccggc
     1141 atcggcgcgt actggttgtg gccgtcgcgc atctgccgcg cgacagactc cacgagctca
     1201 ggggacgtgt cgaaatcggg aaaaccctgc gagaggttga tggcgcccac ctcgttcgag
     1261 aggcgcgtca tcacggcgaa gatgctgacg ccgcccgccg gcaacttcga gcgcacgccg
     1321 ccgagctgcg tcatcgtgcc tatgatagct ggttcactcc agccactcgt gcggcttgag
     1381 cgacaggagc gcgtagcggc gcctgagtgc cacgacgcgg tcgaagtacc ccgtcttggg
     1441 aatctccttg cggacgagac ggtcgcgcag gcgcctgagc tcgtagcgat acaggtcgct
     1501 gacgaactcg tgcacgagct ccggtttcgt cgtgggcgtt ggctggacgc cgtgctgcca
     1561 gagctgctcg aggacgtcgg agcgaaagcg gcggacgctc aaggcctgga tggcctggcc
     1621 ggaggattct gacaggcagg ccgccccaga aacgtacgcg cttcggcgat ctcggccgat
     1681 tcggtctccg gcagcttcca ctcgccaagg agcatccggt attccgcaca ggcggcgttg
     1741 acgtcctcga ggcgggcggc ggtcctggcc aggccgaggt ggacacggct ggtaagaccg
     1801 acctcaccgg ctgcgttcac ataggcgcgc cgcgcatcct cgaaacggtg aacgcgaagc
     1861 cacaggtcgc ccgcggcttc gtgggcgctg acgatcggcg cccccggagc ccccccggca
     1921 cgcagctgcg tctcgaggcg aagcgcgtgg tcgagccgca gcgccatctc ttcgcgct
//
LOCUS       DBNGSH010000991         1953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3026603, whole genome shotgun sequence.
ACCESSION   DBNGSH010000991 DBNGSH010000000
VERSION     DBNGSH010000991.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3026603"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="AC16CY_22660"
     CDS             complement(<1..1157)
                     /locus_tag="AC16CY_22660"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR3356672.1"
                     /translation="MSYRPQAGQLPERGAIPATYTWDLSHICANWDEWQASYRRLDEA
                     IESFTARQGTLAGGTDALLGAFRAMDEMGALSYLVWYYASLRYDEDQRDNEINARRQQ
                     VQILFARQQQSTSWFNPELLAIPLETIRGWMSARPDLAVYRFAIESLFHDQEHVLDER
                     GERLLSYAARSGSVPHDSYSALTTADMTPPVVTLAVGERVTLSYGQYRALLETNRNQA
                     DRVTAYRAFHGVYAGNRNTYAALYNGVLQRDWFHARARGYATTLDAALHGNDIPPSVV
                     ETLIAVTKEGIGPLQRYHRLRRRVLGLDRYHLADVFIPLVEHDRRYAYDQVASWIVDS
                     VAPLGALYQQGVRDAFAGRWIDVFENIGKRSGAYSAPVYGSHPYMLLNYNET"
     gene            complement(1154..>1953)
                     /locus_tag="AC16CY_22665"
     CDS             complement(1154..>1953)
                     /locus_tag="AC16CY_22665"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3356673.1"
                     /translation="EAQMASLGENVSALAVHGTFDDCQRLVKQAFADDRLRQHVWLTP
                     ANSINLGRLLPQVVYYFVLTSLAGPDGSPAPGPIVSVPSGNFGNLTAGLIAKRIGLPI
                     ARFVAATNVNDAVPAYLASGLYEPRPSIRTVANAMDVGAPSNFERVQALYGGDLAALR
                     RDVAGFAVSDDRVTAEIGRVYREHGYLMDPHTTIAWLALRDFQAKQPGVGVFLATAHP
                     AKFREIVEPAIGERVELPAPLAEALARPRQSTSMPAEYGALEEFLRA"
BASE COUNT          328 a          633 c          677 g          315 t
ORIGIN      
        1 gtctcgttgt agttgagcag catgtagggg tgcgagccgt acacgggcgc cgagtaggcg
       61 ccgctcctct tgccgatgtt ttcgaacacg tcgatccatc gaccggcaaa ggcgtcgcgc
      121 acgccctgtt gatacagggc tccgagcggc gccaccgagt ctacgatcca cgatgcgacc
      181 tgatcgtagg cataccgccg atcatgctcg acgagcggaa tgaacacgtc ggccaggtgg
      241 tagcggtcga gcccgagcac ccggcggcgc agccggtggt accgctggag cgggccgatg
      301 ccctccttcg taaccgcgat gagcgtctcg acgacggaag gggggatgtc gttgccgtgc
      361 agcgcggcgt cgagggtcgt ggcgtagccg cgggccctgg cgtggaacca gtcgcgctgg
      421 agcacgccgt tgtagagcgc ggcgtaggta ttgcgattgc ccgcgtagac gccgtggaag
      481 gcacgatagg ccgtcactcg atccgcctgg ttccgattgg tctcgagcag tgcgcggtac
      541 tgcccgtagc tgagcgtgac gcgctcgccg acggcaagcg tgacaaccgg tggcgtcatg
      601 tcggcggtgg tcagcgccga gtagctgtcg tgcggcacac tcccggaccg ggccgcatac
      661 gacagcagcc gctcaccccg ttcgtcgagg acgtgctcct ggtcgtggaa caggctttca
      721 atggcgaagc ggtacacggc aaggtcgggg cgcgccgaca tccatccgcg aatcgtctcg
      781 aggggaatgg ccagcagctc ggggttgaac cacgaggtgg actgctgctg gcgggcgaag
      841 aggatctgca cctgctgacg ccgggcgttg atctcgttgt cgcgctggtc ttcgtcgtag
      901 cgcagcgacg cgtaatacca caccaggtag ctgagggcgc ccatctcgtc catcgcgcgg
      961 aacgcaccga ggagggcgtc ggtcccgccg gccagcgtcc cctgcctggc cgtgaacgat
     1021 tcaatcgcct cgtcgaggcg acggtaactc gcctgccact cgtcccagtt cgcgcagatg
     1081 tgactcaggt cccacgtgta ggtcgccggt atcgcgcctc gttccggcag ctgtcctgct
     1141 tgagggcggt aactcatgcc ctcaagaact cctcgagcgc cccgtactcg gccggcatcg
     1201 acgtcgactg ccgcggtctg gcaagcgcct cggcaagcgg cgccggaagc tcgacgcgct
     1261 caccaatggc cggctcgacg atctcgcgga acttggcggg atgcgcggtg gccaggaaca
     1321 cgccgacacc cggctgcttc gcctggaaat cccggagcgc cagccatgcg attgtcgtgt
     1381 gcgggtccat caggtacccg tgctcccgat aaacgcggcc tatctcggcg gtcacccggt
     1441 catcggacac cgcaaaaccg gcgacgtcac ggcgcagcgc cgccaggtcg ccgccgtaga
     1501 gcgcctggac gcgctcgaag ttgctcgggg ccccgacgtc catcgcgttg gccacggtcc
     1561 gaatggaggg acgaggctcg tagaggccgg acgccagata tgcagggacg gcgtcgttga
     1621 cgttagtggc cgcgacgaac cgggcaatcg gcaggccgat ccgcttcgcg atcaggccgg
     1681 ccgtcaggtt tccgaagttg ccgctgggca ccgagacgat gggaccggga gccggcgagc
     1741 cgtccggccc ggccagagac gtcaggacaa agtagtagac cacctgcggc aggagccgac
     1801 ccaggttgat ggaattggcg ggcgtcagcc agacatgttg gcgcaggcga tcgtcggcaa
     1861 acgcctgctt gacgagccgc tgacagtcgt cgaaggtccc gtgcacggcc agcgccgaca
     1921 cgttctcgcc cagggacgcc atctgggcct cct
//
LOCUS       DBNGSH010000992         3958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3706692, whole genome shotgun sequence.
ACCESSION   DBNGSH010000992 DBNGSH010000000
VERSION     DBNGSH010000992.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3706692"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="AC16CY_22670"
     CDS             <1..269
                     /locus_tag="AC16CY_22670"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR3356674.1"
                     /translation="EIPFAFDNVKAATRLTGGGPDAIALADRVSDAWIAFARTGNPDT
                     PKLPHWPAFNAADRPTMVIDNTSRVEKDPIREQRLLMFRALNYS"
     gene            269..1066
                     /locus_tag="AC16CY_22675"
     CDS             269..1066
                     /locus_tag="AC16CY_22675"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016701 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR3356675.1"
                     /translation="MRMPVVFVPHGGGPWPFVEMGFPQADIDRLAGYLRSVHDVPRRQ
                     PRALLVISAHWEEPVPTVMTAERPSILYDYYGFPPESYAITWPAPGEPQLASRVRSLL
                     DAAGIPNGADGERGFDHGTFIPLKLTYPDADVPAIQLSLKRGLDPSEHLAMGRALAPL
                     RDEGVFIVASGMTFHNLRIFRDPRAVPAAEAFDAWLRETMTLEAVERNRRLEQWTTAP
                     AARTAHPREEHLLPLMVAAGAAGEERASVAFNDTFTGLRLSAYHFGA"
     gene            complement(1372..2217)
                     /locus_tag="AC16CY_22680"
     CDS             complement(1372..2217)
                     /locus_tag="AC16CY_22680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3356676.1"
                     /translation="MIPTAFDYQRATSIDDALAKLKAAGGGKLLAGGHSLVPLMKLRL
                     SQPGTLIDIARIPGLAGIREKDGLIEIGAATTHRDVAASELLRRVCPMIADAAGEIGD
                     PQVRNRGTLGGSLAHADPAADYPAAMLALDAEVHLKGPKGWRKVKASSFFQGLFTVDL
                     AADEIIVGIQFTPIKAAAYAKLHQRASHFAIVGVAAALEVKGGVIQSARIGLTGASTH
                     ATRLTAVEKALAGKPAAKQSIEAASASATAGDLNADIHASEDYRRAMIPVFTKRALEG
                     ALARA"
     gene            complement(2214..>3958)
                     /locus_tag="AC16CY_22685"
     CDS             complement(2214..>3958)
                     /locus_tag="AC16CY_22685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3356677.1"
                     /translation="RGVVAHFEPGKGTMTIWSSTQNPHILRTMIAAMTGMGQDQVRAI
                     APEVGGGFGAKINIYAEEYVAAVVSKKLGIPVKWIEDRAEAFVATTHGRDILAYVDLA
                     AKRDGTVLGLKMRLIADIGAYNMLLTAAIPTLTMLMANATYNIPAIRTTLTEVFTNKT
                     PTDAYRGAGRPEATYFVERAMDMLAAELKMDPADVRRKNFIPADKFPFQTQMGAVYDS
                     GDYAKALDVAIAKSGWNELKKQRDAARAQGRLVGLGLSMYVEVCGIGPSATLPTGGWE
                     HAQVTVERDGRITAATGASPHGQGNETTFAQMIADQLGVPFEHVTVLHGDTGVVKQGI
                     GTFGSRSQAVGGAALHMAGGKAKAKMAKFAAAMLEAHEEDLVFENGTISVKGAPARGK
                     SFAEVAAFAYIPVPLPEGLEPGVSEEAFFEPTNNTYPFGCHISMLEIDRDTGEPTLLK
                     LVAVDDAGNLINPLIVEGQIHGGLAQGIGQAMIEEAVYNSDGQLVTGSFMDYALPRAI
                     DFPRFELHSTVTPTPVNPLGAKGVGEAGTLGSTPSIVSAAVDALSGFGVKHIDMMLRP
                     EKLWRIIHGQGGQA"
BASE COUNT          611 a         1349 c         1314 g          684 t
ORIGIN      
        1 tcgagattcc gttcgcgttc gacaacgtca aagcggccac ccgcctcacg ggtggagggc
       61 cggatgcgat cgcgctggcc gaccgggtca gcgacgcgtg gatcgcgttc gccaggacgg
      121 gaaatccaga cactccgaag ctgccgcact ggccggcctt caacgccgcg gatcgaccga
      181 cgatggtgat cgacaacacg agccgggtcg agaaggatcc gatccgcgag cagcggctgc
      241 tgatgttccg ggcgttgaac tacagctgat gcgcatgccg gtcgtgttcg tgccgcacgg
      301 cggcggcccc tggccgttcg tcgagatggg gtttccccag gcggacatcg atcggctggc
      361 ggggtacctg cgctccgtgc acgacgtgcc ccggcgtcaa ccgagggcgc tgctcgtcat
      421 ctcggcgcac tgggaagagc cggtcccgac ggtcatgacc gccgagcggc cgtcgatcct
      481 gtacgactac tacggctttc cgccggaatc gtacgcaatc acgtggccgg ctcccggtga
      541 gccgcagctg gcctcgcgcg tacgaagcct gctcgacgcc gcggggatcc cgaacggggc
      601 cgatggtgaa agaggcttcg accacggcac gttcattccc ctgaagctga cctatcccga
      661 cgcggatgtt ccggcgattc agctctcgct caagcgcggg ctcgatccct ccgaacatct
      721 ggcaatgggc cgcgcgctcg cgccgctccg ggatgaaggc gtcttcatcg tcgcgagcgg
      781 gatgacgttt cacaacctgc ggatcttccg ggacccgcgc gccgttcccg cggccgaggc
      841 cttcgacgcc tggctgcgcg agacgatgac gctcgaggca gtagagcgga atcgacggct
      901 ggagcaatgg acgacagcgc ctgcggcgcg gaccgcacac ccgcgcgaag agcatctgct
      961 gccgctgatg gtcgcggccg gggcggcggg ggaggaacgc gccagcgtgg ccttcaacga
     1021 cacgttcacc ggccttcggc tatcggcgta tcacttcggg gcgtgagcga cggctgaagc
     1081 cgtttaagtt gatctaccgt agcagcgcac taccgctgca gccaggccag tgtcagccac
     1141 gcgcttgcgc cggcgaccag ccccatagcg agggcttggc cccaggggat agcgcgaccc
     1201 agtcgctttt cacccagcac gtgaccgccg gcgatgccga tcgccgtgcc agcgccgaca
     1261 ccgaaaggcc ccggcacaaa cagcgaagct aaccctgcaa ccgctccgcc cagcagggcg
     1321 ctgacaacca atcgaacggt cattatccgt ggttcgtgaa gatcagcagc gtcacgcccg
     1381 cgcgagcgcg ccctcgagcg cgcgcttcgt gaacaccgga atcatcgccc ggcggtagtc
     1441 ttcgctggcg tgaatgtccg cgttgaggtc gccggccgtt gcagaggccg atgcggcctc
     1501 gatgctctgc ttcgccgccg gcttgcccgc gagcgccttc tcgactgccg tgagacgcgt
     1561 ggcatgcgtg ctggccccgg tcaggccgat gcgtgccgac tggatcacgc cgcccttgac
     1621 ctcgagcgcg gccgcgacgc cgacgatggc gaaatgcgac gcgcgctggt ggagtttcgc
     1681 gtaggccgca gccttgattg gagtgaactg aatacccacg attatctcgt cggccgccag
     1741 atcgacggtg aacaacccct ggaagaagga gctggccttc acctttctcc agcccttcgg
     1801 ccccttgagg tgcacttccg cgtcgagcgc gagcatggcg gcagggtagt cggccgcggg
     1861 atccgcgtgc gcgaggctgc cgccgagcgt gccgcgattg cggacttgcg ggtcgccgat
     1921 ctctcccgcg gcgtcggcga tcatgggaca cacgcggcgc agcagctctg aggcggcgac
     1981 gtcgcgatgg gtcgtggccg cgccgatctc gatcaggccg tccttttcgc ggatgcctgc
     2041 gagcccggga atcctggcga tgtcgatgag cgtccccggc tggctcaggc gaagcttcat
     2101 cagcggcacg aggctgtggc cgcccgccag cagcttgccg ccgcccgcgg ccttcagctt
     2161 cgcgagcgca tcgtcgatcg acgtcgctcg ctggtagtcg aatgctgtgg ggatcacgcc
     2221 tgacctcctt ggccgtggat gatgcgccac agcttctccg gccgcagcat catgtcgatg
     2281 tgcttgacgc cgaagccgct cagtgcatct acggcagccg acacgatgct gggcgtcgag
     2341 cccagcgtac cggcctcgcc cacgcccttc gcgccaagcg ggttcaccgg cgtcggcgtc
     2401 accgtgctgt gcagctcgaa gcggggaaaa tcgattgccc gcggcagggc gtagtccatg
     2461 aacgagcccg tcacgagctg cccgtccgag ttgtagacgg cttcctcgat catcgcctgg
     2521 ccgattccct gcgcgaggcc gccgtgaatc tggccttcga caatcagcgg attgatcagg
     2581 ttgcccgcgt cgtccaccgc gaccagcttg agcagcgtcg gctcgccggt gtcgcgatcg
     2641 atttcgagca tcgagatgtg gcagccgaac gggtacgtat tgttcgtcgg ctcgaagaac
     2701 gcctcttcgc tgacgccggg ctcgagtccc tcgggcaggg gaaccgggat atacgcgaag
     2761 gcggcgacct cggcgaacga cttgccgcgc gccggagcgc ctttcaccga gatggtgccg
     2821 ttctcgaaca cgaggtcctc ctcgtgcgcc tcgagcatcg cggcggcgaa cttcgccatc
     2881 tttgccttcg ccttgccccc ggccatgtga agggccgcgc cgccgaccgc ttgcgagcgg
     2941 ctgccgaacg tgccgatgcc ctgcttcacg acgccggtgt ccccgtgcag gacagtgacg
     3001 tgctcgaacg gcacgccaag ctggtcggcg atcatctgag cgaaggtcgt ctcgttgccc
     3061 tgcccgtgcg gtgaggcgcc ggtcgcggca gtgatacggc cgtcgcgctc gacggtcacc
     3121 tgcgcgtgct cccagcctcc ggtcggcagc gtcgccgatg ggccgatgcc gcagacctcg
     3181 acgtacatcg acaggcccag cccgacgagc ctgccctgtg cccgggccgc gtcgcgctgc
     3241 ttcttcagct cgttccatcc tgacttcgcg atcgcgacat cgagcgcctt ggcgtaatcg
     3301 cccgagtcgt acacggcgcc catctgcgtc tggaatggaa acttgtcggc gggaatgaaa
     3361 ttcttccgcc tcacgtccgc gggatccatc ttcagctcgg ccgccagcat gtccatggcg
     3421 cgctcgacga agtaggtggc ttcgggccgg ccggcgccgc ggtaggcatc ggtgggcgtc
     3481 ttgttcgtga agacctcggt cagcgtcgtg cggatggcgg gaatgttgta cgtcgcattc
     3541 gccatcagca tcgtcagcgt gggaatggcg gccgtgagca gcatgttgta ggcgccgatg
     3601 tcggcgatga gccgcatctt gagcccgagc accgtgccgt cacgcttcgc ggcgagatct
     3661 acgtacgcga ggatgtcgcg cccatgcgtc gtggcgacga aggcctcggc gcggtcctcg
     3721 atccacttca ccggaattcc gagcttcttc gagaccaccg ccgcgacgta ctcctcggcg
     3781 tagatgttga tctttgcgcc gaagccgccg ccgacctcgg gagcgatggc ccggacctgg
     3841 tcctggccca tgcccgtcat cgccgcgatc atcgtccgca ggatgtgcgg gttctgcgtg
     3901 gacgaccaga tcgtcatcgt gccttttccc ggctcgaagt gcgcgaccac gccccgcg
//
LOCUS       DBNGSH010000993         3439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1160115, whole genome shotgun sequence.
ACCESSION   DBNGSH010000993 DBNGSH010000000
VERSION     DBNGSH010000993.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1160115"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="AC16CY_22690"
     CDS             complement(<1..396)
                     /locus_tag="AC16CY_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3356678.1"
                     /translation="MTVLIVGVSSRAAAESAARAGFDVVAIDAFGDLDQHPSVRGLSL
                     PRDFGAPFTPRALVRAASTVAAGAVVYLSTLENHPRTVTALSRGRELWGNASGVLGRV
                     RDPFLLAQVLRTRGFAVPAISGSDPMTRVD"
     gene            complement(393..1484)
                     /locus_tag="AC16CY_22695"
     CDS             complement(393..1484)
                     /locus_tag="AC16CY_22695"
                     /inference="COORDINATES: protein motif:HMM:NF018968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1464 family protein"
                     /protein_id="HIR3356679.1"
                     /translation="MPRVIGIDPGTVSVDLCGLADGRVFLDESIPTPDALGEPSRIVE
                     LLEIASPIDLVAGPSGYGLPVTLAKDLTESDLRLACLAPRGEAGGIGGLAALLRALGR
                     ASMPVVVTPGVVHLTTVPAHRKINRVDMGTADKVCAVALAIHEQARTRRCSERDVSFI
                     LLELGGAFTAAIAVEGGRIVDGLGGSSGPMGPAASGALDAEVAYLAERITKRDLFSGG
                     ALSVAGTPDAPADALADVTTPAGQIAREAYVEDAVKAVVRLTVSAPGARTVVLSGRLA
                     RVAGVRDALSTRLTRSIADISVEVLKGFAASSKHAAQGAAIVADGLAGGASAAIVDRL
                     GIREARGTVLDHLYVISAAAARARLGITG"
     gene            complement(1530..2384)
                     /locus_tag="AC16CY_22700"
     CDS             complement(1530..2384)
                     /locus_tag="AC16CY_22700"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent
                     methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HIR3356680.1"
                     /translation="MKKLLLQIDSSPHPSVFDRVVAFDGGADEVMSYGGVTEDAVRDL
                     VHGAIFTRGPKHLHSTAIFIGGTDMAQGERLLAAVRKAFFGPLRVSAMLDSNGSNTTA
                     AAAVAKMRQASGGLEGRAAVVTAGSGPVGLRAAGLLARAGAHVAVTSRKKADGSLAER
                     IRQRFGGTIRDVTMADASGAASALEGAELLLNAGPAGVCLVPRAAWAGRSGLRVAADV
                     NAVPPSGVEGIEAGDDGVTRDGVSVFGALGIGGLKMKIHKACIARLFERNDLVLDAET
                     IAEIADAL"
     gene            complement(2474..2977)
                     /gene="fae"
                     /locus_tag="AC16CY_22705"
     CDS             complement(2474..2977)
                     /gene="fae"
                     /locus_tag="AC16CY_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HIR3356681.1"
                     /translation="MAADFFLGESLVGDGNEVAHIDLIIGSKTGPAGTAFCNALANNK
                     DGFTTLLAVVAPNLPAKPDTLLFNKVTIKGAKQAVQMFGPAQAAVARAVVDSVESGVI
                     PKDKANDLCILVGVFIHWEASDDKKIFDFNYQATKESIQRALAGKPSVADVLAGAKTA
                     KHPFSPK"
BASE COUNT          599 a         1177 c         1113 g          550 t
ORIGIN      
        1 gtcgactcgt gtcatggggt ctgaccccga tatcgctggc acagcgaaac cgcgcgtacg
       61 gaggacctgc gcgaggagga acggatcgcg cacgcgcccg agcacgccgg atgcgttgcc
      121 ccaaagctcg cgacccctcg agagcgcggt gacggtgcgt ggatgatttt cgagggtcga
      181 cagatacacg acggcgccgg ccgcaaccgt ggacgccgct cgaaccaggg cgcgcggagt
      241 aaacggcgcg ccgaagtccc gcggcagcga aaggccccgc acggatgggt gctgatccag
      301 atcgccaaac gcgtcgatgg cgacgacgtc gaatccggcg cgcgcggctg attcggctgc
      361 ggcgcgcgac gagacaccaa cgatcaggac ggtcatcccg tgatccccag gcgcgcacgc
      421 gcggccgccg ccgagatgac atacagatga tcgagcactg ttccgcgtgc ctcccggatt
      481 cccagccgat cgacgatggc cgccgaagcc ccgcccgcga gcccatccgc gacgatcgca
      541 gccccttgcg cggcgtgttt cgagctggcc gcaaagcctt tcaatacctc gactgagatg
      601 tcggcgatcg accgcgtcaa ccgggtcgac agcgcgtcgc gcactccggc gactcgggcc
      661 agacgccccg acagcacgac ggttcgtgcg ccgggcgccg acaccgtcaa cctgacaacg
      721 gctttcacgg cgtcttcgac gtacgcctcc cgcgcaatct ggccggctgg cgtggtgacg
      781 tcggcgagcg cgtctgccgg cgcatcgggc gtgcccgcga cgctcagagc gccgccgctg
      841 aacaggtcgc gcttggtgat gcgctccgcc agataggcga cttcggcatc gagcgctccc
      901 gacgcagccg gccccatcgg acccgacgag ccgccgagtc cgtcgacgat gcggccgcct
      961 tcgacggcga tggcggcggt gaaggcgcct ccgagctcga gcaggatgaa ggagacgtcg
     1021 cgctcgctgc atctccgcgt ccgggcctgc tcgtgaatgg cgagcgccac cgcgcagacc
     1081 ttgtcggccg tgcccatgtc cacgcgattg atcttgcgat gggcgggcac ggtggtcagg
     1141 tgcacgacgc cgggcgtcac gaccaccggc atcgacgcgc gaccaagcgc tcgaaggaga
     1201 gcggcaagcc ccccgatgcc gcctgcctca cccctcggcg cgaggcacgc gagccggagg
     1261 tccgattcgg tcagatcctt cgcgagcgtg accggcagtc cgtagcctga tggcccggcc
     1321 acgaggtcga tcggggacgc gatctcgagc agctcgacga tccgcgacgg ctcgccgagt
     1381 gcgtcaggag tcgggatcga ctcatcgagg aagacgcgtc cgtcggcgag accgcacagg
     1441 tccacactga cagtacccgg gtcgatgccg atcacgcggg gcattcgaag agcgcccgta
     1501 actcaaaact caagactcaa gactcaaaac tacagagcgt cggcgatttc ggcaatcgtc
     1561 tcggcgtcca gcacgagatc gttgcgctcg aacaggcgcg cgatgcaggc cttgtggatc
     1621 ttcatcttga gtcccccgat gccgagcgcg ccaaagacgc tcactccgtc ccgcgtcacc
     1681 ccgtcgtcgc cggcttcgat gccctcgacg ccactcggtg ggaccgcgtt cacatcggcg
     1741 gcgacgcgca ggcccgatcg accagcccac gccgcacgag ggaccaggca gacgccggca
     1801 gggccggcgt tgagcagcag ctcggcgccc tcgagcgcgg acgccgcgcc agacgcatcg
     1861 gccatagtca cgtcgcggat cgttccgccg aaccgctggc ggattcgttc cgccaacgag
     1921 ccgtcggcct tcttgcgcga tgtcacggcg acgtgggctc cggccctggc gagcaggccg
     1981 gcggcgcgca gccccacggg gccggatccg gcggtgacga cggccgcacg cccctcgagt
     2041 ccaccggagg cctgccgcat cttcgccacg gcggcggcgg cggtggtatt ggagccgttc
     2101 gagtcgagca tcgccgagac gcgcaatgga ccgaaaaacg ccttgcgcac cgcagcgagc
     2161 agccgttctc cctgggccat gtcggtcccg ccgataaaga tcgccgtcga gtggaggtgc
     2221 ttcggaccgc gagtgaagat cgcgccgtgc acgagatcgc ggaccgcgtc ctcggtgacg
     2281 cctccgtagc tcatgacctc gtccgcgcct ccgtcgaacg cgacgacacg gtcgaagacg
     2341 ctcgggtgcg gagagctgtc gatctgcagg aggagcttct tcacgggaga ggatgtgtca
     2401 actcccaagc tccaactccc aactcccaag aggttctcgg cgttgggagt tgggcgttgg
     2461 gagtcgaaga gcgctacttc ggactgaagg ggtgcttcgc cgtcttggcg cctgccagca
     2521 cgtcggcaac cgacggcttg cccgccaagg cgcgctggat cgattccttc gtggcctggt
     2581 agttgaagtc gaagatcttc ttgtcgtcgc tggcctccca gtggatgaac acgccgacca
     2641 gaatgcacag gtcgttcgcc ttgtccttgg ggatgacgcc gctctcgacc gaatccacca
     2701 cggcgcgcgc caccgcggcc tgggcgggcc cgaacatctg cacggcctgt ttcgctccct
     2761 tgatcgtgac cttgttgaac aggagcgtgt ccggcttggc cggcaggttc ggggcgacca
     2821 ccgccagcag ggtcgtgaac ccgtctttgt tgttcgcgag cgcgttgcag aaggccgtcc
     2881 ccgccggccc cgtcttcgag ccgatgatca ggtcgatgtg cgcaacctcg ttgccatcgc
     2941 caaccagcga ctcgccaaga aagaagtccg ctgccatgcc caactcctgt ctggaatgta
     3001 ggtgtgcctt gttgccgggg ccgaggtggc ccgggtctcc tccgcacgcc cgtcatcggc
     3061 ggggcgcccg gagccggacg actatatagg aagaatgcgg gtaattcaaa accggctgac
     3121 gcctgctgaa ccgcccgcct gcgctcgccc cttgcccgcc tgcgactgaa ccgaaacgtg
     3181 gtgccggtcc tacgccttga cctatggtcc caacagtcaa gtacctgtga cgaaacgtta
     3241 tccttgcatt accggatgcg actgatccat gattttgatt cgtaatgttg ctcctggtgg
     3301 atcaacccgt cccaagccaa ggcttccgca gctctaatct caacaaaaca aagccattcc
     3361 gtccgtgggc cgggttggta cgacttttcg ttgtcttttc ccgttaactg agagagcaac
     3421 caagctccac aaaaggaga
//
LOCUS       DBNGSH010000994         1664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4386465, whole genome shotgun sequence.
ACCESSION   DBNGSH010000994 DBNGSH010000000
VERSION     DBNGSH010000994.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4386465"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="AC16CY_22710"
     CDS             complement(<1..1178)
                     /locus_tag="AC16CY_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR3356682.1"
                     /translation="MTTTDTRTTRIAVFIDFDNVEIGVKSTLGAQFDIGLVLEAIKER
                     GEIVTKIAYSDWKRAGDYSRHLTQHAIRMVQRNLTPGGDKNGADITMALDALEMAFTH
                     DHINAFVIVGGDSDFISLVEKLKQYGRKVIVVGGRQFTSVTMQKNCHEFIAYENLVGT
                     RGGGRVADRGGRPSATSDISKALPIVMRALKVLSEREVTPQLGLLKSTLLQLDSTFSE
                     REYGAGSFRDFMEKVAQSGAVTLKHAGRSMLVEVHEEGSTEPEHARGADGHAERAPAA
                     PAPAASGLPEDEDEESMPASPMTMQDGIRAVQQAINRAPAPPRWPMYVRQAKQFLRGA
                     IENFDERHYGFASVVDLLRAAGKEGVLRIERDRQGAVRVFPGANLASRPVAPPVEEPV
                     "
     gene            complement(1471..>1664)
                     /locus_tag="AC16CY_22715"
     CDS             complement(1471..>1664)
                     /locus_tag="AC16CY_22715"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR3356683.1"
                     /translation="KGLLVIHGDQDTVNPIEASKKMADAAKAAGVNAAYATVPGGTHL
                     AAYLTYAKEIFDFLDAQRK"
BASE COUNT          236 a          530 c          571 g          327 t
ORIGIN      
        1 tcgacgggct cctcgacagg cggcgcgacg ggcctcgagg cgaggttcgc gcccgggaac
       61 acgcgcacgg cgccctgccg gtcgcgctcg atccgcagta cgccttcctt gcccgcagca
      121 cgcagcaggt caaccaccga cgcgaagccg tagtgccgct cgtcgaagtt ttcgatcgcg
      181 ccgcgcagga actgcttcgc ctgccggacg tacatcggcc atcgaggcgg agcgggcgct
      241 cgattgatcg cctgctgcac ggcgcgaatg ccgtcctgca tcgtcatggg cgaggccggc
      301 atcgactctt cgtcctcgtc ctcgggcagg cccgatgcgg cgggcgccgg ggccgccggc
      361 gcccgttcgg cgtgtccgtc tgccccgcgg gcgtgctccg gttccgtcga gccttcttcg
      421 tgcacttcga cgagcatgct ccggccggcg tgcttcagcg tgacggcgcc gctctgcgcc
      481 accttctcca tgaaatcgcg gaagctgccg gcaccgtatt cgcgctccga gaaggtggag
      541 tcgagctgaa gcagcgtgct cttgagcagg ccgagctgcg gcgtgacctc ccgctccgac
      601 agcaccttga gggcgcgcat gacgatgggg agcgccttcg agatgtcgct cgtggcagac
      661 ggccggccgc cgcggtcggc gactcgccct ccacctctgg tgccgacgag gttctcgtat
      721 gcgatgaact cgtggcagtt cttctgcatc gtcacgctgg tgaactgccg tccgccgacg
      781 acgatgacct tgcggccgta ctgtttgagc ttctcgacga gcgagatgaa gtcgctgtcg
      841 ccgccgacga tgacgaaggc gttgatgtgg tcgtgggtga aggccatctc gagcgcgtcg
      901 agcgccatgg tgatgtcggc gccgttcttg tcgccgcccg gcgtgaggtt gcgctgcacc
      961 atgcggatgg cgtgctgcgt caggtgccgg ctgtagtcgc cggcgcgttt ccagtcgctg
     1021 taggcgatct tggtgacgat ctcgccgcgc tccttgatgg cctcgaggac gaggccgatg
     1081 tcgaactgtg cgccgagggt ggacttgacg ccgatctcga cgttgtcgaa gtcgatgaag
     1141 acggcgatgc gggtggtgcg tgtatctgtg gtggtcatgt actctctgcc ggcgcgaggg
     1201 ccggactcta acgcgtggag gcgggtgttc ggggctggtc ggacaccgac tcgtaattgt
     1261 cagtcgtcgg attcacggcc tgaggggttc gggctcgagc aagaaatcgg ttcgatttcc
     1321 gcagcacttc tacgagccgt gccccgcagg gcgaagtcta gtacggccgc acgtctgggc
     1381 agccgtcgtt tctattatac gggcttatct gggatccagg ctccggggtc cggtcaaagc
     1441 accggaatcc ccaaccgccg cacgagtgcc tcatttccgc tgggcgtcga ggaaatcgaa
     1501 gatctctttc gcgtaggtca gatacgccgc aaggtgggtt ccgcctggaa cggtggcgta
     1561 ggcggcgttg acgccggctg ccttcgccgc atccgccatc ttcttcgacg cctcgatggg
     1621 attgaccgtg tcctggtcgc cgtgaatcac gagcaggccc ttcc
//
LOCUS       DBNGSH010000995         1695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1500130, whole genome shotgun sequence.
ACCESSION   DBNGSH010000995 DBNGSH010000000
VERSION     DBNGSH010000995.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1500130"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="AC16CY_22720"
     CDS             complement(<1..305)
                     /locus_tag="AC16CY_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356684.1"
                     /translation="MPPLTDAARKREAARAEARRGRGPSDSWEDRSLGDRCIVSRGGW
                     PHVVVNAGSYGNIIRILQSPGYVVLTHEMIHETRIIPVDGRPHISQGIRQYLGDPRGR
                     "
     gene            complement(293..676)
                     /locus_tag="AC16CY_22725"
     CDS             complement(293..676)
                     /locus_tag="AC16CY_22725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356685.1"
                     /translation="MRVIPAVATIVAALCSQAVVGLSAGQAGGSYVAPRTSWGDPDLQ
                     GMWSYNDDVNTPFERPGGPGAKAEFGDEELAAVLEERARRNVERAPTIGGETGAGPTH
                     WYEFWNAKSTRTSKVIDPPDGGCRP"
     gene            complement(700..>1695)
                     /locus_tag="AC16CY_22730"
     CDS             complement(700..>1695)
                     /locus_tag="AC16CY_22730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3356686.1"
                     /translation="GATQFAVSDTGTLVYVPGPATMLSTARSLVIGDARGDPVPLKVP
                     PAVYVHPRISPDGRAVAVGRVDGQSSDIWTYDLSEKVEITRLTFGGVSRFPVWSADSR
                     RVTYQSARDDERGIWWQAASGGTAEQLTKAAKDEEHVPESWSRDGTRLLFSVLKGAKY
                     SLWVLTLDGRKTEPFGKVESEESLSASFSPDGRWVAYAATERAGGTLSPNRGVFVEPF
                     PPTGLKRQAPKRLLDYHPLWAPDGHNIFYVPGSSRPIVSVPVVTRPSVDFGTPVELPR
                     APLPGLISLDVRGYDVLPDGRFLSVSVPQAATGNPSAEVRVVINWFEELKRLAPQ"
BASE COUNT          270 a          580 c          569 g          276 t
ORIGIN      
        1 cggccgcgcg ggtccccgag atactgacga atgccctgac tgatgtgggg gcggccgtcc
       61 accgggatga tgcgggtctc gtgaatcatc tcgtgcgtga gcacgacgta tccggggctc
      121 tgaaggatcc ggatgatgtt gccgtagctg ccggcgttga cgacgacgtg cggccagccg
      181 ccacgcgaca cgatgcagcg atcgccgagg ctgcgatctt cccaggagtc ggagggccca
      241 cggccgcgcc tcgcctccgc ccgggccgcc tcacgtttgc gggccgcgtc ggtcaggggc
      301 ggcacccgcc gtccggcgga tcgatgactt ttgaggtgcg cgtggacttc gcattccaga
      361 actcgtacca gtgcgtcggc cccgcgcccg tctcgccgcc gatcgtgggc gcgcgctcga
      421 cgtttctgcg cgcccgctct tccagcaccg cggccagctc ctcgtcgccg aactccgctt
      481 tcgcgcccgg cccgcccggg cgctcgaacg gcgtgttcac gtcgtcgttg taggaccaca
      541 tgccctggag atccggatcg ccccacgacg tgcgcggggc gacgtacgat ccacccgcct
      601 gaccggcgga gaggccgacc acagcttgtg aacacaaagc cgcgacgatc gtggcgacgg
      661 cgggtatcac gcgcatgggc tcaccattat cgattgaagt tattggggcg ccagccgctt
      721 cagctcctcg aaccagttga tcaccacgcg cacctcggcc gacggattac ctgtcgccgc
      781 ctggggcacg gacacgctga ggaatcgccc gtcggggagc acgtcgtatc cgcgcacgtc
      841 cagtgagatc agccccggca gcggcgcacg cggcagttcg accggtgttc cgaagtcgac
      901 tgagggccgc gtgactaccg gcaccgagac aatgggacgc gacgagccgg gcacgtagaa
      961 gatgttgtgg ccgtcagggg cccacagcgg gtgatagtca aggagcctct tcggggcctg
     1021 acgcttcaag cccgtaggcg ggaacggttc gacaaagacg ccgcggttgg gcgacagcgt
     1081 gccgcccgcc ctctccgtgg ccgcgtaggc gacccatcgt ccatccggcg agaagctcgc
     1141 gctcagcgac tcctcggact cgacctttcc gaagggctcc gtcttcctcc cgtcgagtgt
     1201 gagcacccac aacgagtatt tcgctccctt gaggacggag aacaacagac gcgtgccgtc
     1261 gcgtgaccac gactcgggca cgtgctcttc gtccttggcc gccttcgtca gttgctccgc
     1321 ggtaccgccg ctggcagcct gccaccagat tccccgctca tcgtcgcgag ccgactgata
     1381 ggtcactcgg cggctgtctg cggaccagac gggaaagcgg ctcacgccgc cgaacgtcag
     1441 acgcgtgatc tcgaccttct ccgacaggtc gtacgtccag atgtccgatg actggccatc
     1501 gactcggccg acggcgaccg cccggccgtc gggggagatt ctcggatgga cgtagaccgc
     1561 gggtggaacc ttcagcggga cggggtcgcc acgcgcgtcg ccgatgacga gactgcgggc
     1621 ggttgagagc atcgttgcgg ggccaggtac gtacacgagc gtgccggtgt cggagacagc
     1681 gaactgcgtg gcgcc
//
LOCUS       DBNGSH010000996         7868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4556612, whole genome shotgun sequence.
ACCESSION   DBNGSH010000996 DBNGSH010000000
VERSION     DBNGSH010000996.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4556612"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1833
                     /locus_tag="AC16CY_22735"
     CDS             52..1833
                     /locus_tag="AC16CY_22735"
                     /inference="COORDINATES: protein motif:HMM:NF021578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HIR3356687.1"
                     /translation="MQWTAHDVEEIESELRVLDGVRQRQILHRAYERALRYRLYDALI
                     RHPPRQPPASPAFQAVFCIDEREESFRRHLEEIDPAVETFGTPGFFRVAMYHQSASDA
                     HPRPLCPVVIRPEHYVPEIEQDADAFVRRSLRVQRRAAGFLGYNVHMGSRMLVRGALL
                     TTVFGGLMLVPLILRVVFPWLASRWGRVGDTAPARTRLRLERDETRPPVGKHVGFTVT
                     EMTRIVHNVLEEIGIAGRLSPLVLVVGHGSISLNNPHESAHDCGACGGGRGGPNARAF
                     AQMANDPRVRRQLAADGLAIESSTWFVGAERNTCNNTVTFFDEDLVPVHLQPVLGRAR
                     EAFEATRRQEAHERCRRFDAVPSWYPPLAALAHVEGRAADLAQPRPEYGHATNAYCVI
                     GRRARTSGLFLDRRAFLVSYDPTSDEDGAILSRVMAAVVPVVAGISLEYYFSYVDPAG
                     YGCGTKLPHNVTSLLGVMDGAQSDLRTGLPWQMVEIHEATRLAIVVEASRDRLLRAVQ
                     ANPSIERLVRNRWIWLACLDMHTDALWELRSGGFVAHKPEHSLPTVAGESATWYEGKR
                     GFLPPVAIVREPLPAQVARARHGDVRA"
     gene            1839..3194
                     /locus_tag="AC16CY_22740"
     CDS             1839..3194
                     /locus_tag="AC16CY_22740"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3356688.1"
                     /translation="MTSLEALMLAGVGPPAVLLAVLGGASLLNRPVPERWTGRLAAAA
                     MAISCTAFCVALLLYATTRTGGMVLYGSWSTSHAGGIAIEFLLDGTSLGFAALTSAIA
                     GVVAAFSNRYLHREPGYNRYFFLLAMFVTGMLLVALAGNMEVLFAGWELVGLSSALLV
                     AFFHERPAPVTNALRVFTVYRISDAAMLSAAVLLHHVAGSGSLALLFGGADAASTAGL
                     TGTDATMIAVLLIVAVAGKSALLPFSGWLPRAMEGPTPSSAVYYGSLSIHAGCFLLLR
                     AAPLLEQSPAARLLAGGIGAATALYSSLTTRVQSDVKSSLAYASLTQVGIIVVEIALG
                     LYALAFLHLVGHACFRLLQFLSAPNVLHDLHGLEAAVGERLTPAGGYLETMIPDGVRR
                     RLFLFALERGFVDAVLDRLVVDPFYRVTRLMSRLDRFLCEVVTRPTPAIIVEGGDDRD
                     D"
     gene            3187..4683
                     /locus_tag="AC16CY_22745"
     CDS             3187..4683
                     /locus_tag="AC16CY_22745"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3356689.1"
                     /translation="MIDARLALAGLAALPALVAGVSFLRIDVERLRRLAVASAVVMLL
                     AALAVAVSPDLRGVSIRTSLLSGVPGGEALLRIDALSSALLPFAAGLWLLTVAVTPRA
                     ALDRAGLRRTSLATLVTLASFLTESAIVLLLLWIGSVRIFTSALSDPLHRHQRRVVAT
                     YLGASSLLFAVGVALIVWPMAQGTLFEVVGMWLIVIAALVRKGIFPFHAWVPEVFDHG
                     RLGPAILFSAPQLGAYVTVVLIVPRASPDVLRIIAILALCTAVYGAALALVQTSARRA
                     CGYLFVSQSALVMAGLDCTSVSALAGGLLLWLSAGTAFAGLARCVLVLEARRGRLDLS
                     THHGGYERMPVLAISFLSMGLACTGFPGTLGFAGQELLVDGAVDVFPVLGFAVVIATA
                     LTGLAVLRMYFALFCGRRDAATDSGIHLGLTRREAWTFAVLVMVLVGLGLAPRPVVDS
                     RFTASEEILRQRQARVVTSLEPLEAPSSRVRAPGERLRPGPRADAASP"
     gene            complement(4586..6409)
                     /locus_tag="AC16CY_22750"
     CDS             complement(4586..6409)
                     /locus_tag="AC16CY_22750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR3356690.1"
                     /translation="MAPAGSESWMWWVGERLLSGSVQGAFVVALVWLVCRSIPAIPAS
                     ARACLWWLASLKLALALAPVPSLPVPLLPSGADNAVSVARSLAADALTLPRVAPVTPS
                     SSSQAPTENPARPVVSQSTLDAWLAVAIGLWLAGVAALTVHLLRVYARLRGAVRSSIP
                     IGAEDADVADRLARLLGLRKTPDVRVSDRVTTPLVAGLFRPTVLIPAQALAVLSPDER
                     AMAICHELAHVRRRDLLFGWVPALAERLFFFHPLARLAAREYVVAREAACDTLVLRAM
                     DVAPQEYGRMLVRLGVGGLDPAFMAAGSSPSISSLKRRLDMLHHLTFTGSRRRVIWLL
                     VAVSALTFVPLRLVARAPLQREAAAGVPVLAGAEEQPAKPAPVLDTAQTKPAAPREST
                     DPLLELLFADHTTRVYRADPTLAAQTAEDARQRAEAEYRKAVEEEYRRAVENGHTGDF
                     LKKEYETKLKEYERALLDFTGNQAFTEADRIRQQALAAQRTEVEQLRSKIEEMVQAMK
                     LVAEQREKAAEADRRAMVARIEAERTARTQPGTLDTQSSLARQIEALTVAQQTLIDQL
                     KSLAAQQDQLTAMQRQLSDQVEAVRRELERATRALPTAPAK"
     gene            complement(6414..6815)
                     /locus_tag="AC16CY_22755"
     CDS             complement(6414..6815)
                     /locus_tag="AC16CY_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3356691.1"
                     /translation="MSSQRRLKPVGDQELALLRFVNDAGRATVGQAAERFGQPRGLAR
                     STVLTMMERLRKKRHLTRRQQDGVYHYSPRTAPGAAVRQAVKSFVDRTLEGSIAPFVA
                     YLGERERISDEDLAELEALLARFESQRRKKG"
     gene            complement(6980..>7868)
                     /locus_tag="AC16CY_22760"
     CDS             complement(6980..>7868)
                     /locus_tag="AC16CY_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3356692.1"
                     /translation="ARIRVDTPRDDYAATLNAIVAVVADLERQTGARGTVGVGIPGTL
                     SPSTRLVKNANSVWLIGRPLQSDLEARLGREVRIANDANCFVASEAADGAATGAHVVF
                     GVIVGTGTGAGVSVGGNVITGPNGIAGEWGHNSLPWPDGDETPGPPCYCGKRGCIETF
                     LSGPGMAADHERRTGRREPAPAIVSAALAGDAASLGTLSRYEHRMARALAGVINVLDP
                     DVIVLGGGMSNIERLYENVPRIWREYVFGATRSTPYSQAPNFQTPDGSARVEPVRTRL
                     VRARHGDSSGVRGAAWLWA"
BASE COUNT         1109 a         2705 c         2645 g         1409 t
ORIGIN      
        1 tggccgcagt ttcatttgga gcagctcagc ggcctcgacg actagatcgt cttgcagtgg
       61 acggcgcacg acgtggaaga gatcgaatcc gaactgcggg tgctcgacgg cgtacgacag
      121 cgccagattc tgcatcgagc ctacgaacgc gcgctgcgct atcgcctgta tgacgccctc
      181 atccgccacc cgccgcgcca accgccggcc tcgccagcgt tccaggcggt gttctgcatc
      241 gacgagcgag aggagtcgtt tcggcgtcat ctcgaggaga tcgacccggc ggttgagacg
      301 ttcgggaccc ccggcttctt ccgcgtggcc atgtaccatc agagcgcgag cgacgcccat
      361 ccccgaccgc tctgccctgt cgtcattcgt cccgagcact acgtaccgga aatcgagcag
      421 gacgccgatg ccttcgtgcg gcgatccctg cgcgtccagc ggcgcgcggc aggcttcctc
      481 ggctacaacg tccacatggg gagccggatg ctggtccgcg gagcgctcct gacgacggtg
      541 ttcggcgggt tgatgctcgt ccccttgatc cttcgtgtcg tcttcccgtg gctcgcgtcg
      601 cggtggggtc gagttggtga cacggcgcct gccaggacac gacttcgcct ggaacgggac
      661 gagacgcgcc cacccgtcgg gaaacatgtc gggttcacgg tcacggagat gacgcgcatc
      721 gttcacaacg tcctcgaaga gatcgggatc gccggccgcc tgtcgcctct cgtactcgtc
      781 gtcggacacg gatcgatcag cctcaacaat ccgcatgaat cggctcacga ttgcggcgcc
      841 tgcggaggcg ggcgcggggg gccgaatgcc cgcgccttcg cacagatggc caacgatccg
      901 cgggtgcggc gccagctcgc tgccgacggg ctcgcaatcg aatcgtccac gtggtttgtc
      961 ggggccgagc gcaacacctg caacaacacc gtcaccttct tcgacgagga tctggtccct
     1021 gtccaccttc agccggtgct cggtcgagcc cgtgaggcgt tcgaggcgac gcgacgacag
     1081 gaggcgcacg agcggtgcag gcgtttcgac gcggtgccga gctggtaccc gccgctggcc
     1141 gcgctggctc acgtcgaggg acgggccgcg gatctggcgc agcctcgccc ggagtacggc
     1201 cacgcgacca acgcgtactg cgtcatcggc cggcgtgcgc gcacgtccgg gctgttcctc
     1261 gaccgccgcg cttttctggt ctcctacgat ccgacctccg acgaggatgg ggcgatcctg
     1321 tcgcgggtga tggccgccgt cgtgccggtc gtggccggta tcagtctcga gtactacttc
     1381 agctacgtcg atccggccgg ctacggatgc ggaaccaagc tgccgcacaa cgtcacgtcg
     1441 ctgctcggcg tcatggacgg cgcacaaagc gatctgcgga cgggattgcc gtggcagatg
     1501 gtcgagattc acgaggccac gcgactggcg atcgtcgtcg aagccagccg cgaccgcctt
     1561 cttcgcgccg tccaggccaa tccgtcgatc gagcggctcg tccgcaaccg ctggatctgg
     1621 ctcgcctgtc tcgacatgca caccgacgcg ttgtgggagc tgcgatcggg cgggttcgtc
     1681 gcgcacaagc cggagcactc gttgccgacc gtcgccggcg agtcggcgac ctggtacgag
     1741 ggcaagcgcg ggtttctgcc gcccgtggcc atcgtccggg agccgttgcc ggcacaggtc
     1801 gcccgcgccc ggcatgggga cgtccgcgcg tgacctggat gacctcgctc gaggcgctga
     1861 tgctcgcagg cgtcgggcca ccggcggtgc tgctcgccgt actcggcggc gcctcgctgc
     1921 tcaaccggcc cgtgccagag cgatggaccg gccggctcgc ggcggccgca atggcgatct
     1981 cgtgtaccgc cttctgcgtg gccctcctgc tgtacgcgac gaccaggacg ggagggatgg
     2041 tcctctacgg ctcctggtcg acctctcacg caggcgggat cgcaatcgag ttcctcctcg
     2101 atgggacctc tctggggttc gccgcgctca cgtcggcgat cgcgggcgtc gtggccgctt
     2161 tctcgaaccg gtatctgcac cgcgagcctg gctacaaccg ctacttcttc ctgttggcga
     2221 tgttcgtgac gggcatgctg ctcgtggcgc ttgccggcaa catggaagtc ctcttcgccg
     2281 gctgggaact ggtgggcctc agctcggcgc tgctggtggc gttctttcac gagcggcccg
     2341 ctccggtcac caacgcactc cgcgtgttca cggtctacag aatcagcgac gcggcgatgc
     2401 tgtcggcggc cgtgctgctg caccacgtcg ccggcagcgg cagccttgcg ctgctcttcg
     2461 gaggcgcgga cgcggcgtcg acagcgggac tgaccggaac cgacgcgacg atgatcgccg
     2521 tgctgctcat cgtagcggtc gcgggcaaga gcgcgctgct gcccttttcg ggctggctgc
     2581 cgcgcgcgat ggaagggccg acgccgtcga gcgccgtcta ctacgggtcg ctctcgatcc
     2641 acgccggctg cttcctgcta cttcgtgcgg cgccgctgct cgaacagtcg cccgcggccc
     2701 ggctgctggc tggcggcatc ggcgcggcga cggcgctgta ttcctcgctc acgactcgcg
     2761 tgcaaagcga cgtcaagtcg tcactggcct acgcctccct cacccaagtc ggcatcatcg
     2821 tcgtcgagat tgcgctgggc ctgtatgccc tcgcgtttct ccatctggtg ggtcacgcct
     2881 gtttcaggct tctccagttc ctgagcgcgc cgaacgtgct gcacgacctt cacgggctcg
     2941 aagcggctgt cggcgagcgt ctcacccccg cgggcggcta cctggagacg atgattccgg
     3001 atggcgttcg tcgccgcctg tttctgtttg ccctcgagcg cggattcgtg gatgcggttc
     3061 tcgatcgtct cgtcgtggac ccgttctacc gggtcacgcg actcatgtca cgcctcgatc
     3121 ggttcctgtg tgaggtcgtg acacgaccga cgcccgcgat catcgtcgaa ggcggagacg
     3181 atcgcgatga ttgacgccag actcgcgctc gcaggactcg ccgccctgcc cgcgctcgtc
     3241 gcgggggtgt cgttccttcg catcgacgtc gagcggcttc gccgtctggc cgtggcctcc
     3301 gcagtcgtga tgctcctggc ggccctggcc gttgccgtat caccagatct tcggggcgtc
     3361 tcgatccgga cgtcgctgct cagcggggtg cccggcgggg aagcgcttct tcgcatcgac
     3421 gcgctgtcgt ccgcgcttct tccctttgcc gccggcctct ggctgttgac ggttgccgtc
     3481 accccgcgtg cggcactcga tcgggccggc ctccgacgca cgtcgctcgc cacgctcgtc
     3541 acgctcgcgt cgtttctgac cgaaagcgcc atcgtcctgc tgctgctctg gattgggtcg
     3601 gtccggatct tcacgtccgc tctctccgat ccgctgcacc ggcatcaacg tcgggtcgtc
     3661 gcgacctacc tgggggcctc gtcgctcctg tttgccgtcg gcgttgcgct catcgtgtgg
     3721 ccgatggcgc agggaacgct gttcgaggtc gtcggcatgt ggctcatcgt catcgccgcg
     3781 ctcgtgcgca agggaatctt tccgtttcac gcgtgggtcc cggaggtctt cgaccacgga
     3841 cggctcgggc cggccatcct cttcagcgca ccccagcttg gtgcgtacgt gacagtggtg
     3901 ctcatcgtgc cgcgcgcgtc gcccgatgtg ctgcgtatca tcgcgattct cgcgctctgc
     3961 accgcggtgt acggcgcggc gctggccctg gtgcagacga gcgcccggcg cgcctgcgga
     4021 tatctcttcg tgagccagtc agccctggtc atggccggcc tcgactgcac gagcgtgagc
     4081 gcgctggcgg gcgggctgct gctgtggttg tccgccggga ccgcgtttgc cggacttgcc
     4141 cgctgcgtgc tcgtgctcga agcccgtcgc gggcggctgg acctctccac ccatcacggc
     4201 ggctacgagc ggatgcccgt gctggcgatc agcttcctgt cgatggggct ggcctgcacg
     4261 gggtttccgg gcacgctcgg cttcgccggc caggaactgc tggtcgacgg cgcggtcgac
     4321 gtgttccccg tcctgggatt cgccgtcgtc atcgcgacgg cgctcacggg actggccgtc
     4381 ctgcggatgt acttcgcgct cttctgcggc cgtcgagacg cggcgacaga ctccgggatt
     4441 cacctcggac tcacgcggcg ggaggcgtgg acgttcgccg tactcgtgat ggtgctcgtc
     4501 ggcctgggac ttgcgccgcg cccggtcgtc gactcacggt tcacggccag cgaagagatc
     4561 ctgcggcagc gtcaggcccg tgtcgtcact tcgctggagc cgttggaagc gccctcgtcg
     4621 cgcgttcgag ctcccggcga acggcttcga cctggtccga gagctgacgc tgcatcgccg
     4681 tgagctgatc ctgctgggcc gcgagactct tgagctgatc gatgagcgtc tgctgtgcga
     4741 ccgtgagcgc ctcgatctga cgggcgaggc tgctttgcgt atcgagcgtg cccggctggg
     4801 tgcgcgcggt gcgttcggct tcgatgcggg caaccatcgc cctgcgatcg gcctccgccg
     4861 ctttctcccg ctgctccgcg accaatttca tcgcctgcac catttcttcg atcttgctcc
     4921 gtagttgttc cacttcggtc cgctgcgctg cgagtgcctg ctgtcggatc cgatcggctt
     4981 ccgtgaacgc ctggtttccc gtgaagtcca gaagcgccct ctcgtactcc ttcagtttcg
     5041 tctcgtactc tttcttcaag aagtcacccg tgtgcccgtt ctcgaccgct cggcgatact
     5101 cctcttccac cgccttgcgg tactccgcct cggctcgttg gcgcgcgtcc tcggcggtct
     5161 gggcggccag tgtcgggtcg gcccgataga cacgggtcgt gtggtcagca aacaggagct
     5221 caagaagcgg gtcggtcgat tcgcgtggag cggcaggctt cgtctgggcc gtgtccagga
     5281 ccggcgccgg cttcgcaggt tgttcttcgg cgccagccag gaccgggaca cctgcggcgg
     5341 cctcgcgctg aagcggggcc ctggccacga gccgcaacgg cacgaacgtc agcgccgaca
     5401 cggcgacgag cagccagatc acgcgacggc gggaaccggt gaacgtgagg tggtgcagca
     5461 tgtcgagtct ccttttgagc gacgagatag acggcgaaga gcccgccgcc atgaatgccg
     5521 gatcgaggcc gccgacgccg agccgcacga gcatccgtcc gtactcctgt ggcgcgacgt
     5581 ccatggcgcg cagcaccagc gtgtcgcacg cggcttcccg ggcaacgacg tactcacgag
     5641 cggcaaggcg ggccagcgga tggaagaaaa acagccgctc ggctagcgcc ggtacccacc
     5701 cgaaaaggag atcgcgcctg cgcacgtgcg ccaactcgtg gcagatcgcc atggcacgct
     5761 cgtcgggcga gagcaccgcg agcgcctggg caggaatcag cacggtcggc cggaacaggc
     5821 cggcgacgag cggcgtcgtc acgcggtccg acacgcgaac gtccggcgtc ttcctcaacc
     5881 cgaggaggcg cgcaagtcga tccgcgacat cggcgtcctc ggccccgatt ggaatcgagc
     5941 tccgtactgc gccgcgcaac ctcgcgtaga cgcggagcag atgaacggtc agcgcggcca
     6001 cgccggcaag ccacagaccg atggcaaccg ccagccacgc gtcaagggtt gattgggaca
     6061 caacaggacg cgccgggttc tctgtgggcg cctgagatga cgacgacggc gttaccggcg
     6121 ccacgcgcgg cagcgtcagc gcgtcagcag caagcgagcg ggcgactgac acggcattgt
     6181 ccgcccctga cggcaggagc gggacaggga gcgaaggaac aggcgcgagc gccagggcca
     6241 gcttgaggga ggcgagccac cagaggcacg cgcgcgccga tgcgggaatg gcagggatgc
     6301 tccggcacac gagccagacc agggccacga cgaacgcacc ctgcacggat cccgaaagca
     6361 gccgctcgcc cacccaccac atccacgatt cgcttccagc gggcgccacg tcttcagccc
     6421 ttcttccggc gctggctctc gaagcgcgcc agcagcgctt ccagttcggc gagatcctcg
     6481 tcggagatgc gctcgcgctc gccgagatac gccacaaacg gcgcgatcga tccttcgagt
     6541 gtccgatcga cgaagctctt gaccgcctgc cgcacggccg cgcccggcgc agtccgcggc
     6601 gagtagtgat agacgccgtc ctgctgacgc cgcgtcaggt gtcgtttctt ccggaggcgc
     6661 tccatcatcg tcagcacggt ggaccgcgcg aggccgcggg gctgcccgaa gcgctcggcc
     6721 gcctggccga cggtggcgcg gccggcgtcg ttgacgaacc ggaggagtgc cagctcctgg
     6781 tccccgaccg gcttgaggcg tcgttgacta ctcatgtcgt tactcgagac tgtacacgtg
     6841 acgacagctg tagtcaagac tggtccttcg ttcttggtcc ttcgttcttg gtccttcgtt
     6901 cctggtccgt ccttttgtcc ttggtccatg gtccgctgtc cggaccaagg accaggcacc
     6961 aagaaccaag gacaaccggc tatgcccaga gccatgcggc gccccgcacg ccgctcgaat
     7021 ccccgtgacg ggcgcgcacc aggcgcgtcc gcaccggctc gacgcgggct gacccgtcgg
     7081 gcgtttggaa gttgggcgct tgggagtagg gagttgatct ggttgccccg aatacatact
     7141 ccctccatat ccggggcacg ttctcgtaga gccgctcgat gttcgacatc ccgccgccca
     7201 ggacgatgac gtcggggtcg agcacgttga tgacgccggc gagcgcgcga gccatgcggt
     7261 gttcgtagcg cgaaagcgtg ccgagcgatg cggcgtcgcc cgcgagggcc gccgagacga
     7321 tcgccggcgc cggttcgcgg cgacccgtgc ggcgctcgtg atccgcggcc attccggggc
     7381 cggacaggaa cgtctcgatg cagccgcgct tgccgcagta gcacggcggg cccggagtct
     7441 cgtcgccatc gggccagggc aggctgttgt ggccccactc ccccgcgatg ccgttcggtc
     7501 ctgtgatcac attgccgccg acggagaccc cggccccggt acccgtgccg acgatgacgc
     7561 cgaagacgac gtgcgcgccc gtggccgccc cgtcggccgc ttcggatgcc acgaagcagt
     7621 tcgcgtcgtt ggcgatgcgg acctcgcgcc cgaggcgcgc ctcgagatcg gactggaggg
     7681 gcctgccaat cagccagacc gagttggcgt tcttgacgag acgtgtggac ggcgagagcg
     7741 tgcccggaat gccgacgccg actgttccac gagcaccggt ctgccgttcg agatcggcga
     7801 cgaccgcgac gatcgcgttc agcgtggctg cgtagtcgtc ccggggtgtg tcaacgcgga
     7861 tgcgggcc
//
LOCUS       DBNGSH010000997         6946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4727201, whole genome shotgun sequence.
ACCESSION   DBNGSH010000997 DBNGSH010000000
VERSION     DBNGSH010000997.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4727201"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="AC16CY_22765"
     CDS             complement(<1..385)
                     /locus_tag="AC16CY_22765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356693.1"
                     /translation="MTHRMTGAAIIAAVALSGCGGQPAPGRGGEGELPTLDATHWTDK
                     TELFMEYPPLVAGRPALFAVHLTALADFTPVAAGRAKVEFTPESGGAPRALTGPEPSR
                     PGAFRIEEAPPAPGRYRWALVLEGAA"
     gene            complement(388..1647)
                     /locus_tag="AC16CY_22770"
     CDS             complement(388..1647)
                     /locus_tag="AC16CY_22770"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3356694.1"
                     /translation="MRRTLFCAALAFAFVPSSGGAQDLMLTEADALARLSANSPRVRA
                     LRAGVDVARADVLSSGRWPNPRVNWDRQSVSGVTEHYFTVSQLLPVSGRRDLSVQAAS
                     ARAAAASSRIDDELRRLRTDLRLAFAELDAAQTRVQALVSSRDRLRELADILAKRERE
                     GDAAGFDRLRAEREMLDAEADLIVATTERAQAQATLAAFFENIGEPSQIVAVPRRGRP
                     SQVPTITALMELAETNRGELRALLQDSEAANLLGQAASRSRVPEPELVAGTKSSTATS
                     GGPGSAITVGAGDIGSVFGVHATIPLFDRAQPERTLALSKAAQAEAETAAFRQALRGQ
                     IAALRAAVIDRRNGADRYRTEAVNRTDDIERIARVSYDAGERSILELLDAYRIGASAR
                     VRQAALDLAVRQAELELEFASGWEVPV"
     gene            complement(1705..2103)
                     /locus_tag="AC16CY_22775"
     CDS             complement(1705..2103)
                     /locus_tag="AC16CY_22775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356695.1"
                     /translation="MVPNRPTRRVVAGLAVLGVLGLAVLPQEHVHASHDGEHQDVVHR
                     HLAPHHLFEHDTTVDHGDDDDARYLSAVFIAPGTVSGVDQDPSFVIADLPAVRPAQTR
                     WRTLSFLNVRVHDPPWRTASGPRAPPTPLV"
     gene            complement(2146..3312)
                     /locus_tag="AC16CY_22780"
     CDS             complement(2146..3312)
                     /locus_tag="AC16CY_22780"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR3356696.1"
                     /translation="MLRLHQRYIRRPDLFAAFALVTCLAAAAQAQTQAPNGQKPEPST
                     YDKIWTAFTQFYKDDGNPVVQQVLFSGRYQYEFAAIDADQGDHDEWNVRRLRLGPRIT
                     LLRTLTLHAEVELNPQERDPLYVRITDAYVQWNKSSRLVMTVGKQGVPFTIDGATSSK
                     ELLTIDRSVIGNNIWFPQEYMPGVSVSGQKAPWVYRGGVYSSGAMNPEFGEFNGDYFT
                     LALVGYDFAKRLGAKAAVLTGNYVYQHPDQDNTFTRPLEHTVSVHVRFEDDVWGLRTD
                     LSGATGYLGQSDLWAVTAMPFVNLTPKLQVVGRYSYMGSADPNGIRLGTYENRLVAGR
                     GDEYTELYLGANYYFYGHKLKLQTGLQWADMNDRANDGGEYAGFSWTTGVRVGW"
     gene            3499..>6946
                     /locus_tag="AC16CY_22785"
     CDS             3499..>6946
                     /locus_tag="AC16CY_22785"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3356697.1"
                     /translation="MRLAIRLIVAVACVATVGLFVDSTPARAQGSPTGTLSGSVIDSS
                     GAVLPGVTVIATGAKTGLTQQTISGGAGDWRIPGLPLGVYDVSFELDGFKKLVRSGVT
                     VEASTIRSVPVTLEVGGLTETVQVTGDAALLNITTSATARSLTNAELESVPTSTGSFT
                     HLLSSEAGVSADLPPVLVNGTGNISPSVNGTRTTSTSLFFNGIDATNLTTNEGAMSDN
                     ISPAADTLEEVKLQTSMYDASTGRSGGGNFQLVTRSGTNTLRGTAGFTFQHEGMNAND
                     FFYEKDGIDKPKARRDEGGFTVGGPIRQNRFFFFGGYQRTQAETGFVPTASSISVLPQ
                     ALQLIQGARTKENLLNAFAALNPNILQSIPKAQCSSPTDTACISDVALNLLNLRNPVT
                     GDYVLPAPRAGATMVGNDINAGSAPFVSVGGNPFVRQRNVVPAEFTQDQFTAKLDGEL
                     ATNNRLSVTGFYAKFPGLDPFPDPSSLASPFTLKRDDYNATVAVSDNQIWGASHVNEM
                     RGGIFFLNNSRQLDDPFLAMTNDSVGVPNPANFFDASAATTRLGHYVGRPGGTMERFS
                     FGGPNDTFNKRQQRTYTIGDTLSLTTASHALRMGGEFRHNQYNTNLPEEQATEFEKFD
                     NFTMFLRGLGTEGDTQFGITDKQFRFNDFNMFVADDWRISRTVTINLGMRYEFFGLPT
                     EVNGRIGNVDFDAITDLSNPVNAFIVPNNVQNTGFAAVDAAIAASRRVDNKHTLNGQD
                     WNNVAPRIGFAWTPTEQWVVRGGYGIFYDRPSAAFMNTVFSNYPFLREQEVTFPGAAV
                     PFNSAWSQQDPTYPFNQYLPNQIVRTGGAAGTYQIRDGTNVIRGADGTLNPINPATGL
                     PFRGNIAETFEFRAVDRDLHAAYVQQYNFGVQRSLGANMMVEVRYVGSKGSELLEALA
                     FNQGYDLNDPSTPDHIFERFNQAYVAANSPNGPLNAGATARERGVGRAFGFPNSTLGG
                     MLDYNLANAGGSVIGFEARTPILGFNVPEALLLGNTGRSLYNSVQFGFTKRMSDGLQF
                     NLAYTYSRSMDTSSADPGSTAGGGKPDTPNAGFVVQGDNRDVDANWALSDFDRPHRFS
                     GSWIWNMGAGFRFSGFVQLQSGLPYSIYSVEPEIGSVAQYTDLVRGSGGLYRLGFGRP
                     SLC"
BASE COUNT         1213 a         2356 c         2251 g         1126 t
ORIGIN      
        1 gggccgctcc ttcgaggacg agcgcccagc gatatcggcc gggcgccggc ggcgcctcct
       61 cgatccggaa cgcgccaggc cgggacggct cgggaccggt caacgcgcgt ggcgcgccgc
      121 cggactccgg tgtgaattcg accttcgccc ggcccgccgc gaccggcgtg aaatccgcca
      181 gtgccgtcaa gtgcaccgcg aacagcgccg ggcggcccgc gacgagcggc gggtactcca
      241 tgaacagttc cgtcttgtcc gtccaatgag tcgcgtccag cgtggggagc tcgccctcgc
      301 cgcctcgacc gggagccggc tgcccaccgc acccgctcag cgcgacggcc gcgatgattg
      361 ccgcgccagt cattcgatgt gtcaccgtca tacgggaacc tcccagccgg atgcgaactc
      421 cagttcgagc tccgcctggc gaacggccag gtcgagtgcg gcttgacgca cgcgggcgga
      481 cgcgccgatg cggtacgcgt ccagcagctc gaggatgctg cgctcgcccg cgtcgtagct
      541 cacgcgcgcg atgcgctcga tgtcatccgt ccggttgacc gcctccgtcc ggtagcgatc
      601 tgcgccgttg cggcgatcga tgacggccgc gcggagcgcc gcgatctgcc cgcggagcgc
      661 ctgtcgaaac gccgctgtct cggcctcggc ctgcgcggcc ttcgagaggg cgagcgtgcg
      721 ctccggctgc gcacgatcga acagcgggat ggtggcgtgc acgccgaaca cgctgccgat
      781 gtcgccagcg ccgacggtaa tcgcgcttcc cggcccgccg ctcgtggcgg tcgaggactt
      841 ggtgccggcg accagttcgg gctccggcac gcggctgcgc gaggccgcct gtcccaggag
      901 gttcgcggct tcggaatcct gaagcaacgc ccgcagctcg ccccggttcg tctccgccag
      961 ctccatgagg gcggtgatgg tgggaacctg cgagggacgt ccgcggcggg gaacggccac
     1021 aatctgggac ggctcgccga tgttctcgaa gaaggctgcc agcgtcgcct gggcctgggc
     1081 acgctcagtg gtcgcgacga tcaggtcggc ttcggcatcg agcatttccc gctcggcccg
     1141 gagccgatcg aagcctgcgg cgtccccctc tctctctctc ttcgcgagaa tgtcggccag
     1201 ctcccgcagg cgatcgcggc tgctcacgag cgcctgaact cgagtctgag ccgcatcgag
     1261 ctccgcgaac gccaaccgca gatccgtccg cagccggcgg agctcatcgt cgatccggct
     1321 cgaggcggcc gccgcccgcg cggacgccgc ctgcacgctc agatcccgcc ggccgctcac
     1381 aggcaggagc tgtgacaccg tgaagtagtg ctcggtgacg ccggaaaccg attggcgatc
     1441 ccagttgacg cgcgggttcg gccaccggcc cgaggagagg acgtccgcgc gcgcgacgtc
     1501 gacgcctgct cgaagcgctc gtacacgcgg actgttcgcc gacagccgcg ccagggcatc
     1561 ggcttcggtc aacatcaggt cttgtgcgcc accactggac gggacgaacg cgaaggccag
     1621 cgcggcgcag aacagcgttc tgcgcataac acctcgattg aaaacggatc gtggacagac
     1681 gacggccgat gccgtcgagt caggctagac gagcggagtg ggaggcgcgc gaggaccgga
     1741 cgcagtccgc cacggggggt cgtggacgcg gacgttcagg aacgacagag tccgccagcg
     1801 agtctgtgcc ggccggaccg cgggaagatc ggcgatgacg aacgaaggat cctgatcgac
     1861 gccagagacc gtccctggcg cgatgaagac cgcgctcaga taccgcgcgt cgtcatcgtc
     1921 gccgtgatcg acggtggtgt cgtgctcgaa gaggtgatgc ggcgccagat ggcggtggac
     1981 gacatcctga tgctcgccgt cgtggctcgc atggacgtgt tcctggggca ggaccgccag
     2041 acccagcacg cccagcactg ccaggccggc gacgacgcgc ctagtcgggc ggtttggcac
     2101 cacgaagatt atccggggga tacgcgaccg cgtcaaacct cgccgctacc agccgacgcg
     2161 cacgccggtc gtccatgaga agcccgcgta ttcgcctccg tcgtttgcgc ggtcgttcat
     2221 gtcggcccac tgaagccccg tctgcagctt cagcttgtgc ccgtagaagt agtaattcgc
     2281 gccgagatac agctcggtgt actcgtcccc tcgaccggcg acgagacgat tctcgtacgt
     2341 cccgagcctg atcccgttcg gatccgcgct gcccatgtag ctgtatcgcc cgaccacctg
     2401 gagcttcggg gtcaggttga cgaacggcat ggccgtcacc gcccacaggt cgctctgtcc
     2461 cagatacccg gtcgcgccgg acaggtcggt ccgcaggccc cacacgtcgt cctcgaacct
     2521 gacgtgaacg gacaccgtgt gctcgagcgg ccgcgtaaac gtgttgtcct ggtccggatg
     2581 ctggtagacg taattgcctg tgaggacggc cgcctttgcg cccagccgct tggcgaagtc
     2641 ataacccacg agcgcgagcg tgaaatagtc tccgttgaac tctccgaact ccgggttcat
     2701 ggcgccggac gaatacacgc cgccgcgata cacccagggc gccttctgac ctgaaacgct
     2761 gacgccgggc atgtactcct ggggaaacca gatgttgttg ccgatgacgc ttcggtcgat
     2821 cgtcagaagc tccttggacg aggtcgcgcc gtcgatggtg aacggcaccc cctgctttcc
     2881 gacggtcatc acaaggcggc tgctcttgtt ccactgcacg tacgcgtcgg tgatacgcac
     2941 gtagaggggg tcccgctcct gcgggttcaa ctcgacttcg gcgtgaagcg tgagcgtgcg
     3001 caacagcgta attcgcggac cgaggcgcag ccgccggacg ttccactcgt cgtgatcgcc
     3061 ctggtcggcg tcgatggccg cgaactcgta ctggtagcgg ccgctgaaca gcacctgttg
     3121 gaccaccgga ttgccgtcgt ccttgtagaa ctgagtgaag gccgtccaga tcttgtcgta
     3181 ggtcgacggt tcgggcttct gaccgttcgg cgcctgcgtc tgtgcctgcg cggccgcggc
     3241 aaggcaggtg acaagcgcga acgcggcaaa gaggtcgggc cggcgtatgt atcgctgatg
     3301 taaccggagc atgtcgccaa agtaacgact cgagatgact ggtctgtttc ggcgagatta
     3361 aatccgtgtt acaagcccat cgacggtcat gttcgaggct gcgcgcgagg cgactggcgt
     3421 cctttaacac gtcggcaacc agcgcgttac gtggccgtca cgggcgcccg ataacttcgc
     3481 gccgagcgag gtgattcgat gcgtctggca atccgtctga tcgtcgcagt ggcctgcgtg
     3541 gccacggtgg gtctctttgt tgattccacg cccgcgcgcg cgcagggcag tcccacgggc
     3601 acgctgtccg gctctgtcat cgactcgagc ggcgccgtgc ttccgggcgt cacggtgatt
     3661 gccaccggcg cgaagaccgg cctgacgcag cagaccatca gcggaggcgc gggcgactgg
     3721 cgcatcccgg ggctgccgtt gggcgtctac gacgtgtcct tcgagctgga cggattcaag
     3781 aagctggttc gcagcggcgt gacggtcgaa gcctcgacga ttcggtcggt ccccgtcacg
     3841 ctcgaagtcg gaggtctcac tgaaaccgtt caggtgacgg gcgacgcggc gctgctgaac
     3901 atcaccacgt cagcgaccgc gcgcagtctg acgaacgcgg agctcgaatc ggtcccgacg
     3961 tcgacgggca gcttcacgca cctgctgtcg tcggaagcgg gcgtcagcgc ggacctgccg
     4021 cccgtcctgg tcaacggcac cggcaacatc tcgccatcgg tgaacggcac gaggacgacg
     4081 agcaccagcc tgttcttcaa cggcatcgac gccacgaacc tgacgaccaa cgaaggcgcg
     4141 atgagcgaca acatctcccc ggccgcggac acgctcgagg aagtcaagct ccagacgagc
     4201 atgtacgacg cgtcgacggg ccggtcgggc ggcggcaact tccagctcgt gacacgcagc
     4261 ggcacgaaca cgcttcgcgg gaccgccggc ttcacctttc agcacgaggg gatgaacgcg
     4321 aacgacttct tctacgagaa ggacggcatc gacaagccga aggcgcgccg cgacgagggc
     4381 ggattcaccg tgggcgggcc cattcgtcag aatcgcttct tcttcttcgg gggctaccag
     4441 cgcacgcagg cggaaaccgg tttcgtgccg acggcaagca gcatctcggt gcttccgcag
     4501 gcgctgcagc tgatccaggg cgcccgcacc aaggagaacc tcctgaacgc gtttgcggcg
     4561 ctgaacccga acatccttca atcgattccc aaggcgcagt gttccagccc gaccgatacc
     4621 gcgtgcatct cggacgtggc gctgaatctg ctcaatctgc ggaatccggt gaccggagac
     4681 tacgtgctgc cggcgccgcg cgctggcgcc acgatggtcg gcaatgacat caatgccggc
     4741 tcagccccgt tcgtgtcggt cggcggcaat ccgttcgtgc gccagcgcaa tgtggttccc
     4801 gccgagttca cgcaggacca gttcaccgcg aagctggacg gcgagcttgc gacgaacaat
     4861 cgactcagcg tgaccggttt ctacgcgaag tttcctggac tcgatccgtt ccccgacccg
     4921 tcgagcctcg cctccccgtt cacgctgaaa cgggacgact acaacgcgac ggtcgccgtt
     4981 tcggacaacc agatctgggg cgccagccac gtcaacgaga tgcgaggcgg catcttcttt
     5041 ctcaacaact cccgccagct cgacgacccg ttcctggcga tgaccaacga cagcgtcggg
     5101 gttcccaatc cagccaactt cttcgacgcg agcgccgcca cgacccggct gggccactac
     5161 gtcgggcggc ccggcggcac gatggagcga ttctccttcg gcggtcccaa tgacaccttc
     5221 aacaagcggc agcagcggac ctacaccatc ggcgacacgc tcagcctcac gacggcctcc
     5281 cacgccctgc ggatgggcgg cgagttccgg cacaaccaat acaacacgaa ccttcccgaa
     5341 gagcaggcga ccgagttcga gaagttcgac aacttcacga tgttcctccg cggcctcggc
     5401 acggaaggcg acacgcagtt cggcatcacc gacaagcagt tccgcttcaa cgacttcaac
     5461 atgttcgtcg ccgacgactg gcgcatcagc cggacggtga cgatcaacct gggcatgcgg
     5521 tacgagttct tcggactgcc gaccgaggtc aacgggcgca tcgggaacgt ggacttcgac
     5581 gcgatcaccg acctcagcaa ccccgtgaac gcgttcatcg tgccgaacaa cgtccagaac
     5641 accggcttcg cggccgtcga tgcggcgatc gcagcgtcac ggagagtcga caacaagcac
     5701 acgctcaacg ggcaggattg gaacaacgtg gcgccgcgaa tcggattcgc gtggacgccg
     5761 acggagcagt gggtcgtccg cggcgggtac ggcatcttct acgaccgccc atccgcggcg
     5821 ttcatgaaca cggtgttcag caactatccc ttcctgcggg agcaggaggt caccttcccg
     5881 ggagccgcgg tcccgttcaa cagcgcgtgg tctcagcagg atccgaccta cccgttcaac
     5941 cagtacctgc cgaaccagat cgtgcggacc ggtggcgccg ctggcaccta ccagatccgg
     6001 gacggcacga acgtcatccg cggcgccgac ggcacgctca acccgatcaa tccggcgacc
     6061 ggattgccct tcaggggcaa catcgccgag acgttcgagt tccgcgcggt cgatcgggat
     6121 ctccatgcgg cttacgtgca gcagtacaac ttcggcgtgc agcgcagcct cggcgccaac
     6181 atgatggtgg aagtgcggta cgtcggcagc aaggggagcg agctgctcga ggcgctcgcc
     6241 ttcaaccagg ggtacgacct caacgatccg tctacgccgg accatatctt cgagcggttc
     6301 aaccaggcgt acgtggcggc gaacagcccg aacggtccgc tcaacgcggg cgccacggcg
     6361 cgcgagcgcg gcgtcggcag agcgttcggc ttcccgaact ccacgctcgg cggcatgctc
     6421 gattacaacc tcgccaacgc cggcgggagc gtgatcggct tcgaagcccg cacgccgatc
     6481 ctcggcttca acgtgcccga ggcactgctc ctcggcaaca cgggccggtc gctgtacaac
     6541 tcggtccagt tcgggttcac caagcggatg tcggacggcc tgcagttcaa cctcgcctac
     6601 acgtattcgc gatcgatgga caccagctcc gccgatccgg gaagcaccgc gggaggcggc
     6661 aagcccgaca cgcctaacgc aggcttcgtc gtgcagggcg acaaccgcga tgtcgacgcc
     6721 aactgggcgc tctccgattt cgatcgcccg caccgcttca gcggaagctg gatctggaac
     6781 atgggcgccg ggttccgctt ctcgggcttc gtgcagttgc agtccggtct cccgtactcg
     6841 atctactcgg tcgagcccga gatcggaagc gtggcccagt acaccgacct ggttcggggt
     6901 tcgggcggcc tctaccgcct cgggttcggg cggccgagcc tgtgcg
//
LOCUS       DBNGSH010000998         4800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2519171, whole genome shotgun sequence.
ACCESSION   DBNGSH010000998 DBNGSH010000000
VERSION     DBNGSH010000998.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2519171"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /locus_tag="AC16CY_22790"
     CDS             complement(<1..1973)
                     /locus_tag="AC16CY_22790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3356698.1"
                     /translation="MNDTPGQGSDDADARKVEAVLRQECEQLGRIVDEQERTIAGLRR
                     EVTLHAAAIGWRLQRRLDPLRSWLLSVPVLRQLYRAWYRALEIWVDEGFLQIFARTGN
                     KVDLAVRGRSFLVEDRDRVPHNVEEQYQRWLARHGAPPAGHEMQTAVAGLRHTPLVSV
                     LAVLDAADASRLAHAAGTMRAQGYERWELCLAMPAGVRAALGDDLDRLLGSEPRLRVA
                     EAGGATATCADAYRLARGDLVAVLNVDDELEPHALVELVARLDTEPSADVVYSDEDAI
                     TGEGRRDDPIFKPDWGPDLLLSMNYLERFGVLRRRIVDEAGGFSPDAGLGQSYDLMLR
                     VSEKTDRIAHVPKVLYHARRHPSTVEDVLARHAANRDERRVIDGALERRGRSGRTEAV
                     FARRGPRCYATRYDLASRPLVSIVIPTRDKRALLQTTIESIQARTDYDRYEIIVVDNG
                     SSEPDAVAYLSSLSAPCQVHRWSEPFNYSAINNFGCRHARGEQVLFLNNDVEVIRPDW
                     LAALLEHAQRPEVGAVGAKLLYADGRIQHAGVVVGINRAAGNAFRSWPGQTIGAPRLA
                     DLTRNCSAVTGACMMVPRRVLEKVGGFDETLRVVLNDVDLCLRIRQHGYLVVYTPHAV
                     LYHYEGSTRGLLHPQPDQQAFGEKWKDVLDRGDP"
     gene            complement(1970..3253)
                     /locus_tag="AC16CY_22795"
     CDS             complement(1970..3253)
                     /locus_tag="AC16CY_22795"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3356699.1"
                     /translation="MSSPAIEIEGVSKVYKVYPTPRHRALELLTFGARTYHTDLCALR
                     DVTLRVDRGTTLGVVGNNGSGKSTLLQVVAGISTPTTGRVAVHGRISSLLELGAGFNP
                     EFSGRENVELYGAVVGMTREETRARFPAIEAFAGIGEAIDRPVKSYSSGMFVRLAFSA
                     AIHVDPDILLVDEALAVGDGAFQHQCLRRIRELQAGGTTILFVSHDMAMVRAICTDAV
                     LLHGGRVEARDHPATIVDLYHARTSALEAAQLAPRGPRRETGASGAVPFHVGKDFDER
                     VKAYRHGTGAARIRQVELIDAEGRPISAVHFNEQVVLRVHVEYYEDAPLSILGFGFRD
                     RTGVDIIGTNTHEERVDLPPRRAGDTLVVDFRQHLPLAKGAYSVSTALAYDPARPTYF
                     DWVDHALLVTVLPPESGKVVHGKVWVPVEISVHAS"
     gene            complement(3253..4080)
                     /locus_tag="AC16CY_22800"
     CDS             complement(3253..4080)
                     /locus_tag="AC16CY_22800"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356700.1"
                     /translation="MPITRGIVGSAVVSPFRTLVVHRDVIEIFVRRDVQSRYINSALG
                     LWWAVIQPLALLALFTFVFSGIMKMRLGERDGTGEFALYLFCGLLPWLAFSDAVTRSS
                     SVIVEQTPLIKKVLFPSEILPVHLVLSALVVEAAGLAVFLAAMVALGRPPGWALAALP
                     VVIALQVVFTAGVAWLMAAVAVFLRDTRHVVGLGLTLWLFLTPIMYSDSLVPDRYRWI
                     LAVNPMAYVVEAYRSALLQNELPAAVPSAVFAGVAFAAFVSGHWVFVRSKRAFADLL"
     gene            complement(4112..>4800)
                     /locus_tag="AC16CY_22805"
     CDS             complement(4112..>4800)
                     /locus_tag="AC16CY_22805"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3356701.1"
                     /translation="LNNLGAARARGALLCFLNNDTEVISPDWLEELVSLASREEIGAV
                     GATLYHPDDTVQHAGIALGLGGIAAHRFRGLPRGEAGNSGLLALTQAVSAVSAACMMV
                     RKSVFDEVGGFDEALAVAYNDVDLCLRLGRRGLRNVWTPFAELYHFESATRGTDVRGE
                     ARARLLAEAQVVRQRWRDVLGADPYFNPNLSLERPDFALAYPPRHTASWWQVRSAARD
                     TLDEPRTSVR"
BASE COUNT          826 a         1646 c         1612 g          716 t
ORIGIN      
        1 ggatcgcccc gatcgagaac gtccttccac ttctcgccga acgcctgctg gtccggctgc
       61 ggatgcagca ggccgcgtgt cgatccctcg tagtgataca agaccgcatg cggcgtgtat
      121 acgacgaggt acccgtgctg ccggatgcgg agacagagat cgacgtcgtt gagcacgacg
      181 cgcagcgtct cgtcgaagcc gccgaccttc tccaggaccc gtctcggcac catcatgcag
      241 gcgcccgtga ccgcgctgca gttgcgtgtc agatcggcga gccgcggcgc accaatcgtc
      301 tggccgggcc acgatctgaa cgcattgccg gcggctctgt tgatgccgac cacgacgccc
      361 gcgtgctgga tgcgcccgtc cgcgtacagc agcttggcgc cgacggcgcc gacctccggc
      421 cgttgtgcat gctcgagcag cgccgccagc cagtccgggc ggatcacctc cacgtcgttg
      481 ttgaggaaca gcacctgttc tccgcgcgcg tggcgacacc cgaagttgtt gatcgccgaa
      541 taattgaacg gctcgctcca ccggtgcacc tggcacggag cggagagcga cgagagatag
      601 gcgaccgcat cgggctccga gctgccgttg tcgacgacga tgatctcgta gcggtcgtag
      661 tccgtgcgcg cctggatact ttcgatcgtg gtctgaagga gggcgcgttt gtcgcgtgtg
      721 ggaatcacga tcgagacgag cggcctcgac gcgagatcgt acctcgtggc atagcagcgt
      781 ggcccgcgcc gcgcgaatac cgcctcggta cgcccggacc ggcctcgtcg ttcgagcgcg
      841 ccgtcaatga cgcggcgctc gtcgcggttg gccgcgtgcc tcgccagcac atcctccacg
      901 gtcgatggat ggcgccgagc gtgatagagc accttgggca cgtgcgcgat ccggtccgtc
      961 ttctcgctca cgcgcagcat cagatcgtag gactgcccca gaccagcatc gggcgagaac
     1021 ccgccggcct cgtccacgat gcgcctgcgc aggaccccga atcgctccag gtagttcatc
     1081 gagagcagca gatccgggcc ccagtcgggc ttgaagatcg gatcgtcccg gcggccctcg
     1141 cccgtgatcg cgtcttcgtc cgagtagacg acgtcggctg acggctccgt gtcgagccgc
     1201 gcgaccagct ccacgagagc gtggggctcg agctcgtcgt ccacgttcag cacggcaacg
     1261 aggtcgccgc gggccagacg atacgcgtcg gcgcacgtcg ccgtggctcc gccggcctcc
     1321 gcgacgcgga ggcgaggctc gctgccgagg agccggtcga ggtcgtcccc gagcgccgcc
     1381 cggacgccgg cgggcatcgc gaggcacagc tcccagcgct cgtagccctg ggcccgcatc
     1441 gtgcctgccg cgtgggcgag ccgcgaggca tcggcggcat ccaaaacggc cagcacactg
     1501 acgagcggtg tgtggcgcaa gcccgccacc gccgtctgca tctcgtgacc ggccggcggc
     1561 gccccgtgcc gcgcgagcca gcgttgatac tgctcttcca cgttgtgcgg cacacggtcg
     1621 cggtcctcga cgaggaaact gcggccgcgc acggcaaggt cgaccttgtt tccggtacgg
     1681 gcgaagatct gcaggaaccc ttcgtccacc cagatctcga gggctcgata ccacgcgcgg
     1741 tacaactgcc gtagaaccgg gacgctgagc agccaggatc ggagcggatc cagccgacgc
     1801 tgcagccgcc acccgatcgc ggcggcgtgc agggtcacct cgcggcggag gccggcgatc
     1861 gtccgttcct gttcgtcgac gatccgcccg agctgttcgc actcctgccg caggaccgcc
     1921 tcgaccttcc gtgcgtcggc gtcgtcagaa ccctgcccgg gcgtgtcgtt catgacgcgt
     1981 gcaccgagat ctcgacgggc acccagacct tcccgtgaac caccttgccc gactcgggcg
     2041 gaaggaccgt caccaacagg gcgtgatcga cccagtcgaa gtacgtcggt ctggcgggat
     2101 cgtaggcgag cgcggtgctg acgctgtagg cacccttcgc cagggggagg tgctggcgga
     2161 aatcgacgac cagcgtgtcg ccggcgcgcc ggggtggcag gtcgacccgc tcctcgtgcg
     2221 tgttcgtccc gatgatgtcg acgcccgtcc tgtctcgaaa accgaagccg aggatgctca
     2281 atggagcgtc ctcgtagtac tccacgtgca cccgcaggac gacctgctcg ttgaagtgca
     2341 cggcgctgat cggcctcccc tctgcgtcga tgagctcgac ctggcggatc ctggcggcgc
     2401 cggtgccgtg ccggtatgcc ttgacccgct cgtcgaagtc tttcccgacg tggaacggga
     2461 cggcgccgct cgcgcccgtc tcgcggcgcg gcccgcgcgg cgccagctgt gctgcctcga
     2521 gagccgacgt ccgggcgtgg tagagatcga cgatcgtggc ggggtggtcc ctggcctcga
     2581 cgcgtccgcc gtggagcagt acggcatcgg tgcagattgc ccgcaccatg gccatgtcgt
     2641 ggctgacgaa gagaatcgtc gtgccgccgg cctgcagctc ccggatccgg cgcaggcact
     2701 ggtgctggaa cgcgccgtcg ccgacggcca gcgcctcatc gacgagcagg atgtcggggt
     2761 cgacgtggat ggccgcggaa aacgcgagcc gcacgaacat cccgctcgaa tacgacttca
     2821 caggacgatc gatggcctcg ccgatgcccg caaatgcctc gatcgccgga aagcgcgccc
     2881 gtgtctcctc gcgcgtcatg ccgacgacgg cgccgtacag ctcgacgttc tcacggccgc
     2941 tgaactcggg gttgaagccg gcccccagct ccagcagcga cgagatccgc ccatggacgg
     3001 cgacccggcc ggtcgtgggc gtcgagatgc ccgcgacgac ctgcagcagc gtgctcttcc
     3061 ccgaaccgtt gttgccgacg acgccaagcg tggtaccgcg atcgacgcga agcgtgacgt
     3121 cacgcagcgc gcacaggtcg gtgtggtacg tcctggcccc gaacgtgagg agctcgagcg
     3181 cccgatggcg gggagtcggg tagaccttgt agaccttcga gacgccctcg atctcgatcg
     3241 cgggtgagct catcagagca gatccgcgaa ggcgcgcttc gatcggacga acacccagtg
     3301 tccggacacg aatgccgcga aagcgacgcc cgcgaagacc gcagacggca ccgcagcggg
     3361 cagctcgttc tggagcaggg cgctccggta cgcctctacg acgtacgcca tcgggttcac
     3421 ggccaggatc cagcggtagc gatcgggtac cagcgagtcg gaatacatga tcggcgtgag
     3481 gaacagccac agggtaaggc cgagacccac cacatggcgc gtgtctcgga ggaacacggc
     3541 gacggcggcc atcagccagg cgacgccggc ggtgaacacg acctgcagcg cgatcacgac
     3601 gggtagcgcg gcaagcgccc agcccggagg ccggccgagc gcgaccatgg cggcgagaaa
     3661 caccgcgagt ccggcggcct cgacgaccag cgcggagagc accagatgca cgggcaggat
     3721 ctccgaagga aacagcactt tcttgatgag cggcgtctgc tcgacgatga ccgacgacga
     3781 gcgggtaacg gcatcggaaa acgccagcca gggcagcagc ccgcagaaca ggtacagcgc
     3841 gaactcgccg gttccgtcgc gctctcccag gcgcattttc atgatgcccg agaagacgaa
     3901 cgtgaacagc gccagcagcg cgagcggctg gatcacggcc caccacaggc cgagagcgga
     3961 gttgatgtag cggctctgaa cgtcccggcg aacgaaaatc tcgatcacgt cgcgatgaac
     4021 gacgagcgta cggaacggag acacgacggc gctgccgacg atcccgcgag tgatcggcat
     4081 tctcagcgag tcagtatagt tcgcgcgctg gtcatcggac actcgttcgt ggttcgtcga
     4141 gcgtgtcgcg agccgccgaa cgtacctgcc accacgaggc cgtgtgccgc ggcggatacg
     4201 cgagcgcgaa gtccggtcgc tcgagcgaga gattagggtt gaaatacggg tccgcgccca
     4261 gcacgtccct ccagcgttgc ctcaccacct gggcctctgc gagaagcctg gctcgcgcct
     4321 cgccacggac atccgtcccc cgggtggcgg actcgaagtg atacaactcc gcgaacggag
     4381 tccacacgtt ccgcagaccg cgtcttccga gccggagaca cagatccacg tcgttgtagg
     4441 cgacggcgag cgcctcgtcg aaccccccga cttcgtcgaa cacgctcttg cggaccatca
     4501 tgcacgcggc ggagacagcc gacacggcct gggtgagcgc caacagaccg gagttcccgg
     4561 cctcgccgcg cggcagtccc cggaaccgat gggcggcgat gcccccaagc cccagggcga
     4621 tcccggcgtg ctgaacggtg tcgtcggggt ggtagagcgt cgcaccaacc gccccgatct
     4681 cctcccggct ggcgagactg accagttctt cgagccagtc cggagaaatg acctcggtgt
     4741 cgttgttcag gaaacacaac aaggcgccgc gcgcccgggc ggcgcccagg ttgttgagcc
//
LOCUS       DBNGSH010000999         2196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3707980, whole genome shotgun sequence.
ACCESSION   DBNGSH010000999 DBNGSH010000000
VERSION     DBNGSH010000999.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3707980"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="AC16CY_22810"
     CDS             <1..1581
                     /locus_tag="AC16CY_22810"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3356702.1"
                     /translation="ARFLAPHYVGMILADLPDPKPTRVVTTLDAGLQRAIEGIVRSQR
                     ALLERHGAKNVAVVVLDNTTSQWLAWEGSGNFGSPDRGGSINGAIAPRQPGSALKPFT
                     YALAFEQGFSPASVLADVPSSFPTAEPGVVYSPRNYDGRYRGPLLARRALAGSENVPA
                     VVLASELGVPALLRFLGRAGLSTFDKTAAYYGLGVTLGNAEVRLDELTAAYAAFARGG
                     EWIEPAFVRRSAGAAPSTRQLVSARSAFWVTDILADPEAREFVFGRGGSLELPFPVAV
                     KTGTSQAYHDNWAIGYSHRVTVGVWVGNFDRTPLRNSSGVTGAGPIFNAVMLAAERRL
                     GGSSPAFPDAPIRPVPGNVVEREICALSGMAANAWCPLRRREWIGAEAPSQPCHWHHL
                     SDEGLLTFWPPEYRQWARTQGLDEAPRARARDVMAYGASSADDRRVASRAPLTIVSPA
                     ADSTYLIDPTLRREFQTLPLRVVASTSGPIEWTVSGRAVGTANADAALDWPLSTGRHR
                     IVARDRQGRTAETMVTVR"
     gene            1711..2082
                     /locus_tag="AC16CY_22815"
     CDS             1711..2082
                     /locus_tag="AC16CY_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3356703.1"
                     /translation="MTPDDLKARTKKFAVDVIRFAKSIPHDPIHNEIAVQLTDAATSV
                     AAHYRAVCRARSRADFINKLSGAVEEADESALWLEILKESGICHGAATAPLWQEADEL
                     TRILVRSRETARANRQSGNRK"
BASE COUNT          370 a          731 c          719 g          376 t
ORIGIN      
        1 gcgcgattcc tcgcgccgca ctacgtcggg atgatcctcg ccgatctgcc cgatcccaag
       61 ccgacgcgcg tcgtgaccac gctcgacgcc gggctgcagc gcgcgatcga gggcatcgtc
      121 cgcagccagc gcgctctcct cgagcgccac ggtgcgaaga acgtcgccgt cgtcgtcctc
      181 gacaacacga cctcgcagtg gctcgcctgg gaaggctccg gcaacttcgg gtcgccggac
      241 cgcggcggat cgatcaacgg cgccatcgcg ccccgccaac cggggtcggc gctcaagccg
      301 ttcacctacg cgctcgcgtt cgagcagggg ttcagccccg cgagcgtact ggccgacgtg
      361 ccctccagtt ttccaaccgc agaacccggc gtcgtctaca gcccgcgcaa ctacgacgga
      421 cgataccggg gcccgctgct cgcacggcgc gcgctcgccg gatcggagaa cgtgccggcc
      481 gtcgtcctgg cgtcggagct gggcgtcccg gcgctgctgc ggttcctcgg gcgcgccggg
      541 ttgagcacgt tcgacaagac ggcggcctac tacgggctgg gcgtcaccct gggcaacgcg
      601 gaggtgaggc tggacgagct gacagcggcc tacgcggcct tcgcgagagg cggcgagtgg
      661 atcgagccgg ccttcgtgcg ccgctccgcc ggagccgcgc cgagtacgcg gcagctggtg
      721 tcggcgcgct cggcgttctg ggtgaccgac atcctcgcgg atcccgaggc tcgcgagttc
      781 gtcttcggcc gcggcggcag cctcgagctg ccgtttcccg tcgccgtgaa gacgggcacg
      841 tcgcaggcct accacgacaa ctgggcgatc ggctactcgc accgcgtcac ggtcggcgtg
      901 tgggtgggca acttcgatcg gacgccactc agaaattcca gcggagtcac cggcgccggg
      961 ccgatcttca acgcggtgat gctcgccgcc gagcggcgtc tcggcggatc gtcgcccgcg
     1021 tttcccgacg cgcccattcg accggtgccc ggcaacgtcg tcgagcgcga gatctgcgcg
     1081 ctctccggca tggcagctaa cgcgtggtgt ccgttgcggc gtcgcgagtg gatcggcgcc
     1141 gaggcgccgt cgcagccgtg ccactggcat cacctgagcg acgagggcct gctgacgttc
     1201 tggcctcccg aataccgtca gtgggcgcgc acccaggggc tcgacgaggc gccgcgagcc
     1261 cgcgcgagag acgtcatggc gtacggggct tcctcggccg acgatcggcg tgtcgcatca
     1321 cgcgcgccgt tgacgatcgt cagtcctgct gcggattcca cctacctgat cgatcccacg
     1381 ctgcgccggg agttccagac gctgcccctg cgtgtggtcg cctcgacgag cggaccgatt
     1441 gaatggaccg tgtccggccg ggcggtgggc acggccaatg ccgatgcggc cctcgactgg
     1501 ccgctctcca cgggtcgtca ccgcatcgtg gcgcgcgatc ggcaagggcg taccgcggag
     1561 acgatggtga ctgtgaggta gattgaagat tttgcgatct ttgattgttg attcgttggc
     1621 gatttttgat tgttgattgg ggatttattg gcgattactt gtttttgccc atgattattg
     1681 attggcacgg ccggtgctcg ccaatggggc atgacaccag acgatctgaa ggcccgcaca
     1741 aagaagttcg cagttgacgt gatccgcttc gccaaatcca ttccacacga tcccatccac
     1801 aacgagatcg ccgtacagct gactgacgca gcaacatccg tcgcggcgca ctaccgtgcc
     1861 gtttgccgcg cgcggtcgcg cgccgatttc atcaacaagt tgagcggggc cgtggaggaa
     1921 gccgacgaat cggcgttgtg gctcgagatc ctgaaggagt ccggcatctg tcacggagcc
     1981 gcaacagcgc cgctgtggca ggaagccgac gaactgacgc gtatcctggt ccgctcccgc
     2041 gaaacggcca gggcgaatcg acaatcaggc aatcgcaaat agatcgaaaa tcaagaatct
     2101 ccaatcaata aggaatcaag aatcaacaat cccaaatcaa gaatcaacaa tggtctagtt
     2161 acaccgtcgc caccgggcgc atcttcgcct cgggat
//
LOCUS       DBNGSH010001000         2569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2519297, whole genome shotgun sequence.
ACCESSION   DBNGSH010001000 DBNGSH010000000
VERSION     DBNGSH010001000.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2519297"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2569
                     /locus_tag="AC16CY_22820"
     CDS             <1..>2569
                     /locus_tag="AC16CY_22820"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3356704.1"
                     /translation="LAALVDRARIAARRGDEPRLRGSRDALAQLTSSWSPQAIEQFMA
                     LERAIEARRFEEAARATTFLRNVLTPVPAFRESLAAVRTPAELIAEPFDRFLVLAPPE
                     AEPSPPDASITFSAEAAGGDAASGLSSLIAMTPTIEDPPLLFAADAGSLRRLDGSPAS
                     WPVVSSRGGSLDPPALAALDWNHDFRTDIVAAGRGGVRVFLQDEQSGFADATAKVAGG
                     APVADAVFGAWPADVEMDGDIDIVVGSREGQTFVMRNNGDTTWRRIQPFGGLGAVRGF
                     GWADLDGDADADAVFLGADNALHLFANRQAGVFSRMENVSGVAGVVAVAVADIDADGG
                     VDVVALESSGVIRRISRQGAGWISTEIASWQGLDSAGPGTHRLLVADLDNNGALDVIA
                     AGGGASRIWLAGADHTLQLLPAPPPAEVFAGIDRNGDGRIDLAALMDGRPSWLMNGGG
                     KAGYHWKVIRPRGQQRAGDQRINSFGVGGSISVRSGLLVQTQIMTGTPVHFGLGMHTT
                     VDVARIVWPSGIAQAEFGTGADEVMAAEQRLKGSCPWVFAWDGSRMTFVTDFLWRSPL
                     GLRINAQDTAGVGQTEDWIRIAGSQLVPRDGFYDVRITAELWETHFFDHVSLMVVDHP
                     KDTEVFVDERFRPAQPPALAVRSMRMLGPVAKARDELGRDVTDVVAARDGRYLASFER
                     GPYQGIAQEHYVELDVAGRIPSRGATLVASGWIYPTDSSINVAIGQGGHVKASGVALE
                     ALVDGRTWVVLTPDVGYPAGKDKTMLIDLERAGKATRLRLRTNLEVYWDSFALAEEVE
                     TAMRTARLSVEESELRYRGFSETTSPRGDAPETPDYSRIATASQRWRDLVGFHTRFGN
                     "
BASE COUNT          387 a          832 c          932 g          418 t
ORIGIN      
        1 ctggcggcgc tcgtcgatcg cgccaggatt gcggccaggc gcggcgacga accccgtctc
       61 cgcggttccc gtgacgcgct cgcgcaattg acgtcgagct ggtcgcccca ggcgatcgag
      121 cagttcatgg cgctcgagcg cgcgatcgag gcgcgccgct tcgaggaggc cgcgcgtgcc
      181 actactttcc tgaggaacgt gctgacgccg gtgccggcgt ttcgcgagag cctggccgcc
      241 gtgcggacgc cggccgagct gattgcggag ccgttcgatc gtttcctcgt gttggcgccg
      301 ccagaggctg agccatcgcc gcctgatgcg tcgatcacgt tctccgccga ggcggcgggc
      361 ggtgatgcgg cgagcggatt gtcctcgctg atcgcgatga cgccgacgat cgaggatcca
      421 ccgctgctgt ttgccgctga tgcgggcagc ctcaggcggc tcgacggatc tcctgcgtca
      481 tggccggttg tctccagtag aggcgggtct ttagacccgc ctgccctcgc ggcgctcgac
      541 tggaaccacg acttccgcac ggacatcgtc gccgccggcc gcggcggcgt gcgcgtcttc
      601 cttcaggacg agcagagcgg gttcgcggat gccaccgcga aggtcgcagg gggtgcgccc
      661 gtcgccgacg ccgtctttgg agcctggccc gccgacgtcg agatggacgg cgacatcgac
      721 atcgtcgtgg ggagcaggga aggccagacg ttcgtgatga ggaacaacgg cgatacgacg
      781 tggcggagaa tccagccatt cggcggcctc ggcgccgtgc gtggcttcgg atgggcggat
      841 ctcgacggcg atgccgacgc ggatgccgtg tttctgggcg ccgacaacgc gcttcacctc
      901 ttcgcgaacc ggcaggccgg agtcttctct cgaatggaga acgtgagcgg tgttgccggc
      961 gtggtcgccg tcgccgtcgc ggacatcgac gccgacggcg gggttgatgt cgtcgcgctc
     1021 gagtcgagcg gcgtcatccg gaggatctcg cggcaggggg cgggttggat cagcaccgag
     1081 atcgcgtcgt ggcaggggct cgattccgca gggcccggca cccaccgcct cctggtcgcg
     1141 gatctcgaca acaacggcgc cctcgacgtg atcgcggccg gaggcggagc gtcgcggatc
     1201 tggcttgccg gggcggacca tacgctgcaa ctcctgccgg cgccgccgcc ggcggaggtc
     1261 tttgccggca tcgatcgaaa cggcgacggg cgaatcgatc tggccgccct gatggacggg
     1321 cggccgagct ggctgatgaa cggcggaggg aaagcgggct atcactggaa ggtgatccgt
     1381 ccgcgcggcc agcagcgcgc cggcgaccag cggatcaatt cgttcggcgt cggcggctcg
     1441 atcagcgtgc gctcgggcct cctcgtccag acacagatca tgacgggtac gccggtccac
     1501 ttcggactcg gcatgcacac gaccgtggac gtcgcccgca tcgtctggcc cagcggcatc
     1561 gcgcaggccg agttcgggac cggcgccgac gaagtcatgg cggccgagca gcgcctcaag
     1621 ggttcgtgtc cgtgggtgtt cgcctgggac ggcagtcgaa tgacgttcgt gaccgacttc
     1681 ctgtggcggt cgccgctcgg tctccggatc aacgcgcagg acactgcggg cgttggtcag
     1741 acggaggact ggatcaggat cgcgggcagc cagctggtgc cgcgagacgg tttctacgac
     1801 gtgcggatca ccgcggagtt gtgggagacg cacttcttcg atcacgtctc gctgatggtg
     1861 gtggatcacc cgaaggacac ggaggtcttc gtggacgagc gcttcaggcc ggctcaaccg
     1921 cctgcgctcg cagtgcgctc catgcgcatg ctcgggccgg tcgccaaggc gcgcgacgag
     1981 ttgggccgcg acgtcaccga cgtcgttgcc gcacgtgacg ggaggtacct cgcttctttc
     2041 gagcggggtc cgtaccaggg gattgcgcag gagcactacg tcgagctcga cgtcgccggg
     2101 cggatcccct cgcgcggggc cacgctcgtc gcgagcggat ggatctatcc gaccgacagc
     2161 agcatcaacg tcgccatcgg ccagggcggg catgtgaaag cgagcggcgt ggcgctcgag
     2221 gcgctcgtcg atggacggac ctgggtcgtc ctcactccgg acgtcggcta ccccgcgggc
     2281 aaggacaaga cgatgctcat cgacctcgag agggctggga aggcgacgcg actccgcctc
     2341 cgcaccaatc tcgaggtcta ctgggactcg ttcgccctgg ccgaggaagt cgaaacggcg
     2401 atgcgaacgg ccaggctttc tgtcgaggag tcggaactgc ggtaccgcgg gttctccgag
     2461 acgacgtcac cgagaggcga cgccccggag accccggact actcgcggat cgcaaccgcg
     2521 tcccagcggt ggcgcgacct cgtgggtttc cacacgcgct tcggtaatg
//
LOCUS       DBNGSH010001001         1888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3368780, whole genome shotgun sequence.
ACCESSION   DBNGSH010001001 DBNGSH010000000
VERSION     DBNGSH010001001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3368780"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1095)
                     /locus_tag="AC16CY_22825"
     CDS             complement(214..1095)
                     /locus_tag="AC16CY_22825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3356705.1"
                     /translation="MLRSSVAATVTVLLGLAALSAQAPQGGRGGRGPAAGQTVEKIRE
                     LKPNLYMITGGGANTLVRVTTDGLIVVDTKNPGDENYKRVMEEIASVSPLPVKYVLNT
                     HHHPDHVGNNQKFIDAGAQVVGLEALKTHMTSDPRTKEIPGPPTITFAKDYVLKLAGA
                     VVEAHAFGRGHTGDDTMVYFPDAKVVMVSDQITNATPIVDFANGGSAVEWTKVLDGVL
                     KLDFEMAIPGRGDPKTRAEVQAFRTNFAALVSRASDAIKAGAGRDQLAAQVKTDDLGW
                     QFNPQFFGQLYDELTKK"
     gene            complement(1241..>1888)
                     /locus_tag="AC16CY_22830"
     CDS             complement(1241..>1888)
                     /locus_tag="AC16CY_22830"
                     /inference="COORDINATES: protein motif:HMM:NF019040.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3356706.1"
                     /translation="DEEKRVLNAMSQPRETTNATLKFLASTAQNATESSDFVRRASSS
                     YRTTVDYGSGGGLGGNLQRVAALIAAQMPTRLYYVTYSGNNFDTHVQQADLHTRLLMY
                     TADAVRGFMEDIRRIGREDEVAVMIFTEFGRRVEENGSLGTDHGTATPMFVLGQGVKG
                     GFYGRHPSLTDLDDGNMKMTTDFRRVYATMIEEWLGCREAQAILKGRFEPLGVFA"
BASE COUNT          284 a          647 c          595 g          362 t
ORIGIN      
        1 gcggcggtgg atcccgtggc ggccgcggca tcgccgcact tgatttcgac gctggcgtcc
       61 ttgacgccct ggcctcccat gacgatcgtc ccgtacacct gtcccgcgtc ggcgacggcc
      121 ggcagcaaca gcgcgacgaa cagcgccacg acagtagtgg ttcgcatcga catcctccgg
      181 aatcggctca tggaatgact gtacaccgtc cggctatttc ttcgtcagct cgtcgtagag
      241 ctggccgaag aactgcgggt tgaactgcca gcccagatcg tcggtcttga cctgcgccgc
      301 gagctgatca cggcccgcgc ccgccttgat cgcgtccgat gcgcggctga cgagcgcggc
      361 gaagttcgtc cggaacgcct ggacctcggc gcgcgtcttc ggatctccgc ggccgggaat
      421 ggccatctcg aagtcgagct tcagcacgcc gtccagaacc ttcgtccact cgacggcgct
      481 cccgccgttc gcgaaatcga cgatcggcgt ggcgttggtg atctggtcgc tgaccatcac
      541 caccttcgcg tccgggaagt acaccatcgt gtcgtcgccc gtgtgcccgc ggccgaaggc
      601 gtgcgcttcg accacggcgc cggccagctt gagcacgtag tccttcgcga aagtgatcgt
      661 cggcggaccg ggaatctcct tcgtccgcgg atcgctcgtc atgtgcgtct tcagcgcctc
      721 gaggcccacg acttgcgccc cggcgtcgat gaacttctgg ttgttgccga cgtgatcggg
      781 gtgatggtgc gtgttgagca cgtacttcac gggaagcggc gaaaccgacg cgatctcttc
      841 catgacgcgc ttgtagttct cgtcgcccgg attcttcgta tcgacgacga tcaacccgtc
      901 agtggtcacg cggaccagcg tgttcgcgcc tccgccggtg atcatgtaga ggtttggctt
      961 cagttcccgg atcttctcga ccgtctgtcc tgcagcgggg ccccgaccgc cgcgcccgcc
     1021 ctgcggggct tgggccgaca gcgcggcgag accgagtaac accgtgacgg tggcggcgac
     1081 agacgaacga agcatgtgtt cccccttgaa tcggacagtc cggctgccgc cttcgcgcga
     1141 cgcgcttcgg cgcccaagga agccggacac tacgcgcgat cacggtccgg ctgaagccgg
     1201 acactacgta ctaccgattg gggtcttcgt gtcttcgtgg ctacgcgaag acgcccagcg
     1261 gctcgaagcg ccccttcagg atcgcctgcg cctctctgca cccgagccac tcctcgatca
     1321 tcgtggcgta cacgcgcctg aagtcggtgg tcatcttcat gttgccgtcg tccagatcgg
     1381 tgaggctcgg atgacgcccg tagaagccgc ccttgacgcc ctggccgagg acgaacatcg
     1441 gcgtcgcggt gccgtgatcg gtgccgaggc tcccgttctc ttcgacgcgc cgcccgaact
     1501 cggtgaagat catcaccgcc acctcgtcct cgcgcccgat gcgcctgatg tcctccatga
     1561 atccccgcac ggcatcggcc gtgtacatca ggagccgcgt gtgcaagtcg gcctgctgca
     1621 cgtgggtgtc gaagttgttc cccgagtagg tgacgtagta caggcgcgtc ggcatctgcg
     1681 cggcgatgag cgccgcgacc ctctgcaggt tcccgccgag cccgccgccg ctcccgtaat
     1741 ccaccgtcgt ccggtaggac gacgaggcgc ggcgaacgaa gtcggagctc tcggttgcgt
     1801 tctgcgccgt cgacgcgagg aacttgagcg tcgcgttcgt cgtctcgcga ggctggctca
     1861 tcgcgttgag cacgcgcttc tcttcgtc
//
LOCUS       DBNGSH010001002         2123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2690186, whole genome shotgun sequence.
ACCESSION   DBNGSH010001002 DBNGSH010000000
VERSION     DBNGSH010001002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2690186"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="AC16CY_22835"
     CDS             complement(<1..1011)
                     /locus_tag="AC16CY_22835"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 isoprenoid biosynthesis enzyme"
                     /protein_id="HIR3356707.1"
                     /translation="MASMPAAAALHSPSLASRLTPLRDACIADWHATPPVLPRLGSPS
                     TRWQKWRNGRATGRLIDDIARRVDGYPESVSERREWRESVRTRLQDFGEAHFHWPSGY
                     RRLLFGDAFYEASVDFARRARQFDPALSLDDLWQALRNVWIGNSLQMLLARQVALTPG
                     LFAYSMLYPVTDNLLDSPDVPSEDKRLFNRRLGRRLAGDRLEAGRQAEEHAFALVRQI
                     EETLPRHLYPDVWESVLAIHRGQIDSLRQHGARLSRTELVEVSFAKGGASVLADLYLV
                     AGSATAHEERFAFGYGVFLQLLDDLQDVSSDRAAGHQTLFTHALNDGRLDEETARLAR
                     FID"
     gene            complement(1061..1558)
                     /locus_tag="AC16CY_22840"
     CDS             complement(1061..1558)
                     /locus_tag="AC16CY_22840"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3356708.1"
                     /translation="MALVDALLPEFDHEMSLTRKILERVPEDRLDWKPHQKSYSLGQL
                     AQHVATLPMWGSVTLNESEIDIGGSERPAPIDSRSALLKLFDGHVEGTRAALVGKTDA
                     ELRVPWTLKNSGHVVFSMPKASVWRSFVISHIIHHRAQLGVYLRMQDVPLPAMYGPTA
                     DEPGF"
     gene            complement(1575..>2123)
                     /locus_tag="AC16CY_22845"
     CDS             complement(1575..>2123)
                     /locus_tag="AC16CY_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3356709.1"
                     /translation="VFALSVTALASSGCSYNNFVGQEEAIKTQWAQVENQLQRRNDLI
                     PNLVESVKGIMQQEQDVFGRIADSRAKLSGATTPEQRMQAANEQSAALARLLVVMENY
                     PQLRSNESLARLMDELSGTENRIAVERMRYNEKVQAYNTLRRSFPSNITGGLFGFKEH
                     ALFDAPKEAESVPRVNFGKPGA"
BASE COUNT          348 a          692 c          704 g          379 t
ORIGIN      
        1 atcgatgaac ctcgccaggc gcgccgtctc ctcgtccagc cggccgtcgt tcagcgcgtg
       61 cgtgaacagc gtctgatggc cggcggcccg gtcggaggag acgtcctgca ggtcgtccag
      121 caactgaagg aacacgccgt agccgaacgc gaaccgctcc tcgtgtgcgg tcgcgctgcc
      181 cgcaacgagg tacagatccg cgaggacgga cgcgcccccc ttggcgaagc tgacctcgac
      241 cagttcggtg cgtgacagcc tcgcgccgtg ctggcgcagg ctgtcgatct gtccgcgatg
      301 aatggcgagc acgctttccc acacgtccgg atagaggtgg cgtggaagtg tctcctcgat
      361 ctggcggacc agtgcgaagg cgtgctcctc tgcctggcgg ccggcctcga gcctgtcgcc
      421 cgccagccgg cgtcccaggc gtcgattgaa gagacgcttg tcctccgacg gcacgtccgg
      481 ggaatcgagc aggttgtcgg tgaccggata cagcatgctg taggcgaaga gtcccggagt
      541 cagcgccacc tggcgcgcca ggagcatttg aaggctgttg ccgatccaca cgttgcgcag
      601 tgcctgccag aggtcgtcga gcgacaacgc cggatcgaac tgccgtgctc tcctggcgaa
      661 atcgacggag gcctcgtaga aggcgtcgcc gaacagcagg cggcggtagc ccgacggcca
      721 gtgaaagtgc gcctcgccga aatcctggag ccgcgtgcgg accgattccc gccattcgcg
      781 acgctcggaa acggattcgg ggtagccgtc gacacgccgt gcgatgtcgt cgatcaaccg
      841 gccggttgcc ctcccgttgc gccacttctg ccagcgcgtg gacggggagc cgagccgggg
      901 cagcacggga ggcgtggcgt gccagtcggc gatgcacgcg tcacggagcg gcgtcaatcg
      961 actggcgagg ctcggcgagt gcagggccgc ggcggctggc atcgatgcca ttggcatcaa
     1021 ctacgcgaca gcgcgcggaa ggttcctgag cctcttccag ctagaagccg ggctcgtcgg
     1081 cggttgggcc gtacatcgcg ggcagcggca cgtcctgcat gcgcaggtac acgcccagct
     1141 gcgcgcgatg gtgaatgatg tggctgatga cgaaggaccg ccacaccgat gccttcggca
     1201 tcgagaacac gacgtgcccg ctgttcttga gcgtccacgg cactctgagc tctgcgtccg
     1261 tcttgccaac gagcgccgcg cgcgtgcctt cgacgtgtcc gtcgaacagc ttcaatagag
     1321 cggaccggct gtcgatcggc gcgggacgct cggagccgcc gatgtcgatt tccgattcgt
     1381 tcagcgtgac ggatccccac atcgggagcg tggcgacgtg ttgggcgagc tgccccagcg
     1441 aatacgactt ctgatgcggc ttccagtcca agcgatcctc gggcacgcgc tcgaggatct
     1501 tgcgcgtcag cgacatctcg tggtcgaact cgggcagcaa cgcgtctaca agcgccatga
     1561 cagacctctc gtgctcaggc tcccggtttt ccgaagttga cccgcggtac gctttcggcc
     1621 tccttcggcg cgtcgaagag cgcgtgctcc ttgaatccaa agagcccgcc cgtgatgttc
     1681 gatgggaagc tcctgcgaag cgtgttatac gcctggacct tctcgttgta acgcattcgc
     1741 tcgacggcga tgcggttctc ggtgcccgac agctcgtcca tcaagcgtgc gagcgactcg
     1801 ttcgatcgca gctgcgggta gttttccatg acgacgagca gccgggcgag cgcggcgctc
     1861 tgctcgttgg ccgcctgcat ccgctgttca ggcgtcgtcg cgccggacag cttggcgcgt
     1921 gagtcggcga ttcggccgaa gacatcctgc tcctgctgca tgatgccctt caccgactcc
     1981 accaggttcg ggatcaggtc gttgcgccgc tggagctgat tctccacctg cgcccattgc
     2041 gtcttgatgg cctcttcctg gccgacgaag ttgttgtacg aacaccccga cgaggcgaga
     2101 gccgtcacgg acagcgcgaa cac
//
LOCUS       DBNGSH010001003         2203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2182389, whole genome shotgun sequence.
ACCESSION   DBNGSH010001003 DBNGSH010000000
VERSION     DBNGSH010001003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2182389"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="AC16CY_22850"
     CDS             <1..870
                     /locus_tag="AC16CY_22850"
                     /inference="COORDINATES: protein motif:HMM:NF024946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR3356710.1"
                     /translation="LRAQTIDDGIMLSDGELQAGGLFSYDTWNEYWEGTLKRVNGNIG
                     TITTSTNLWTANYAVSDRLNVMGMVPYVWTRASLGVLHGIQGFQDLTLAAKWSGFEKP
                     APASGTLRGILVVSAGIPMSNYNLELLPLSIGTGSTWVSGRGTVNFQSGPGWFLNGSA
                     AYTWRSGVELDRPYFYTDDEFFMSSEVDMPNTFDYVASAGYMKNGLMAAFAFSQLQTL
                     GGGDIRRQDMPIPSNQMNFSKVGGMLMYPIPKLRPLAFRLAVAQVLSGQNVGEATTVT
                     TGLMYTFNGKAGR"
     gene            867..>2203
                     /locus_tag="AC16CY_22855"
     CDS             867..>2203
                     /locus_tag="AC16CY_22855"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR3356711.1"
                     /translation="MKRSRTRLLWFLAASASMAVAPSTIGQTAASTDPSAGNWRMILL
                     SGPTQFTVPPPGQTSGADYQAELNAIKSAQSRITTAQRKAIDYWGRGGAVRWNEIMLG
                     LVARYNLPPAPAPGDTYPVPDANNPFADPPFPFANPPYAARAYSYVAAAQYDALKAAW
                     HFKHLYNRPAPWKVDSGVKALGPVTDLPAYPSEDAVMSGVNAELLKLLFPASVQEITL
                     KAAEQRQAAVLSGRATASDVAAGLALGQAVAAVFTARAGTDGMRTAGGSPALWQALAD
                     AARARGEIPWRSMETPPRPPMLPLFGSVRTWMMTADDIARERPGPPPSTSSALMAQEV
                     AEVRRYVDSGTREELRIANFWADGPGTPTPPGHWNYLALPYLLKAKYSEVRTARALAL
                     LNMAMHDAAVACWDAKFAYFNPRPSQMDPELKTIIGLPNFPSYTSGHSTFSSAA"
BASE COUNT          381 a          736 c          733 g          353 t
ORIGIN      
        1 ctgcgcgccc agaccatcga cgacggcatc atgctgtcgg acggtgaact gcaggccggg
       61 ggcctcttct cctacgacac ctggaacgag tactgggagg ggacgctcaa gcgcgtgaac
      121 ggaaacatcg gaacgatcac gaccagcacc aatctctgga ccgccaacta cgcggtttcc
      181 gatcgactca atgtgatggg gatggtcccg tacgtgtgga cccgggcgag cctgggcgtt
      241 ctgcacggca tccagggctt ccaggacctc acgcttgccg ccaaatggag cgggttcgag
      301 aaacccgcgc cggcctccgg cacgctgcgg ggcattctcg tcgtctcggc gggaattccc
      361 atgtcgaact acaacctgga gctgctgccg ctctcgatcg gaacgggcag cacgtgggtg
      421 tcgggccgcg gaacggtgaa tttccagtcg ggtccgggat ggttcctcaa cggatcggcg
      481 gcctacacct ggcgctcggg cgtcgagctc gatcgcccgt acttctatac cgacgacgag
      541 ttcttcatga gcagcgaggt ggacatgccc aacacgttcg actacgtggc gagcgcgggc
      601 tacatgaaga acggcctgat ggccgccttc gcgttttcgc agctgcagac gctcggcggc
      661 ggcgacatcc ggcgtcagga catgcccatc ccgtccaacc agatgaactt ttccaaggta
      721 ggcggcatgc tcatgtaccc gatcccgaaa ctgcggccgc tggcgttccg gctggccgtg
      781 gcgcaggtgc tcagcggcca gaacgtggga gaggcgacga cggtcaccac gggcctcatg
      841 tataccttca acggaaaggc tggccgatga agcgttcgcg tacgcgtctc ctctggttcc
      901 ttgcggcgtc ggcgtcgatg gccgtggcgc cgtcgacgat cggccagact gctgcgagca
      961 ccgatccgtc tgcgggcaac tggcggatga tcctgctgtc cggtccgacg cagttcaccg
     1021 tgccgccgcc cggccaaacc agcggcgcgg actaccaggc ggagttgaac gcgatcaaga
     1081 gcgcgcagtc gcgcatcacc accgcgcagc gcaaggccat cgactactgg ggccgcggcg
     1141 gcgccgtgcg ctggaacgaa atcatgctcg ggctggtcgc gcggtacaac ctgcctccgg
     1201 cgccggcccc cggcgacacc tatccggttc ccgacgcgaa caacccgttt gccgatcctc
     1261 cgttcccgtt cgccaatccg ccgtacgccg cgcgcgccta cagttacgtg gccgccgcgc
     1321 agtacgacgc gttgaaggcg gcgtggcact tcaagcacct ctacaaccgc cccgcaccat
     1381 ggaaggtgga cagcggcgtc aaggcgctgg ggccggtgac cgatctgccg gcctatccgt
     1441 cggaagatgc ggtcatgtcc ggtgtcaacg cggagctctt gaaactgctc tttccggcat
     1501 ccgtgcagga gatcacgctc aaggcagccg aacagcggca ggccgcggtc ctgtccggga
     1561 gagccactgc aagcgacgtc gcggcgggcc tggccctggg gcaagctgtc gcggcggtct
     1621 tcactgcgcg agccgggacg gacggcatga ggaccgccgg gggctcaccc gcgctgtggc
     1681 aggcgctggc cgatgcggca cgggcgcgag gcgagattcc gtggaggagc atggagacgc
     1741 cgccgcggcc gccgatgctc ccgctgtttg gcagcgtccg cacctggatg atgacggcgg
     1801 acgacatcgc gagggaacgg cccgggccgc ctccatccac gtcgtcggcg ctgatggcgc
     1861 aggaagtggc ggaagtgcgg cgatacgtcg atagcggcac gcgcgaggag ctcaggattg
     1921 ccaacttctg ggcggacggc cccggcacgc caacgcctcc aggccactgg aattacctgg
     1981 cgctgccgta cctcctgaaa gccaagtaca gcgaggttcg gactgcgcgc gcgctggcgc
     2041 tgctgaacat ggccatgcac gatgccgccg tcgcatgttg ggacgcgaag ttcgcctact
     2101 tcaacccgcg cccctcgcag atggatccgg agctcaagac gatcatcggc ctcccgaact
     2161 ttccgtcata cacgtcgggg cactcgacct tctcgagcgc ggc
//
LOCUS       DBNGSH010001004         4484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_994026, whole genome shotgun sequence.
ACCESSION   DBNGSH010001004 DBNGSH010000000
VERSION     DBNGSH010001004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_994026"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..274)
                     /locus_tag="AC16CY_22860"
     CDS             complement(50..274)
                     /locus_tag="AC16CY_22860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 domain-containing protein"
                     /protein_id="HIR3356712.1"
                     /translation="MRGLVIAGVLLIVLGLVALASRGVTYTSRETVVDIGPLHATADQ
                     QRTLPLPPVLGLAGVAAGVALVIAGVRKHA"
     gene            complement(324..992)
                     /locus_tag="AC16CY_22865"
     CDS             complement(324..992)
                     /locus_tag="AC16CY_22865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356713.1"
                     /translation="MKRLLILTVGAALGVAACGTTASDATDTTPGGNATPPGAARQPP
                     DRPVYRAVTIPVGTTLPLALMTAVASDTSAVEDAVTAELTRPVTIDGQDVLPAGARLT
                     GVVTDVDDSARVKGKAMIAFRFTSLHLGDKQYDVQSATVSHVAPGTKVQDATKIGIGA
                     GAGAIIGGVLGGKSGAATGAAVGAGAGTGVVLATKGPEVRLGPGADVTTELTAPLTVR
                     IRLS"
     gene            1368..2114
                     /locus_tag="AC16CY_22870"
     CDS             1368..2114
                     /locus_tag="AC16CY_22870"
                     /inference="COORDINATES: protein motif:HMM:NF012948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356714.1"
                     /translation="MLTRIVASALAVLTLGIGGTAQAQPADYRTYFTFSAPVTLPGVT
                     LPAGRYLFRLADPSTSRKVINVMSADGRTSLAMLLTIPNKLAKAPENAEIRFMETPAN
                     TPSAIKTWWYPGNSIGYEFIYPRQQALLLAKVNSEPVLTTVAETRDLEKAELARISRE
                     GVPAPVVVDENPPLVVATGRVQQGEVVTGGGTVTSSVQDPRADERARTTRTALPQTAS
                     SLPTVLLFGTLAMLAGLGLSLRRRASVYAD"
     gene            2168..3100
                     /locus_tag="AC16CY_22875"
     CDS             2168..3100
                     /locus_tag="AC16CY_22875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3356715.1"
                     /translation="MCWPCVAVVTVAILLLPGCVNVRGQGPPVFSADSELVVLHVTVK
                     DRTGAYVTGLPQEAFSIVEDGRAQTVRLFIDIDTPVTVGLLIDSSASMHPHRRLVITG
                     ATSFAEASHPRDEIFALAFNEDVHPVLSPTAPFTGNSTVLHAALERSVTARGRTALYD
                     AISAGLDYLSRGTRERKVLVVLSDGGDNASRATREESVRRALASNAVIYTIGLVALGE
                     SDANPTLLKELAQASGGESFRPQAPREIAAVLGQISRNIRHTYTVGYTPTNTARDGAF
                     RRVRVVVTAPPGRPRPLAVRSRAGYVAGTTKVEP"
     gene            3097..3681
                     /locus_tag="AC16CY_22880"
     CDS             3097..3681
                     /locus_tag="AC16CY_22880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HIR3356716.1"
                     /translation="MTRLVQRWLARLLIAGGAMALMWVAANLVWTVSVRRQYEATLER
                     IRSAPAAEEPVAPVSLPVGEPIGTLEIARIGLSGVVVEGDDDSVLDRAIGHLPDTPLP
                     WHTGNSALAAHRDTLFRPLRGVRPGDVLRLKTPHGDFDYVVRETVIVKPDDLWVLDPT
                     PVSMLTLISCYPFNFIGNAPKRFIVRAERMPRFL"
     gene            3790..>4484
                     /locus_tag="AC16CY_22885"
     CDS             3790..>4484
                     /locus_tag="AC16CY_22885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3356717.1"
                     /translation="MTRIDGRLIVFVAMLAGGGVLLGGQTPQTPPDQQSPLPTFRAAV
                     EAVQLSVIVTDGDGKPVTGLTAADFEIFENRAPRAITTFSAVDIPVERSERALADPDV
                     LGNDRPPGRLYVIAFDAITPVNALRARVFLRQFIENYFGPNDTAAVVLTTRGPRDSGQ
                     DFTSNPRLLLNAIDRFGGDQPDGWDSTMRERNFIGDFRNLMAFMATLPGARKAMIYVT
                     ESIPVDAYDVVDAR"
BASE COUNT          757 a         1529 c         1436 g          762 t
ORIGIN      
        1 ggtcgcactt gcagccacgg acctcggcct cgggttcacc acgtcaccgc tacgcgtgct
       61 tgcgaacccc ggcgatcacc agcgccacgc cggcggccac ccccgccagc cccagcaccg
      121 gcggcagcgg cagcgtccgc tgctgatccg ccgtcgcgtg caggggaccg atgtcgacga
      181 ccgtttcgcg actcgtgtag gtcacgcccc gagaggcgag cgccaccagg ccgagtacga
      241 tgagcagcac gccggcgatt accagacctc tcatgggatc ctcatgtcgg tgcgcttcct
      301 ggtcgctgtg acccgcgtga cgatcagctc aggcggatgc gcaccgtcaa cggcgccgtg
      361 agctccgtcg tgacgtcggc gcccggtccg aggcgcactt ccggcccctt cgtggcgagg
      421 acgacacccg tcccggcacc ggcccctacc gccgcgcccg tggcggcgcc actcttcccg
      481 cccaggaccc cgccgatgat cgcgccggcg ccggcgccga tgccgatctt cgtggcgtcc
      541 tgcaccttag tgcccggtgc cacatgcgac accgtcgcgg actgcacgtc gtactgtttg
      601 tcgcccagat gcaatgacgt gaagcggaac gcgatcatcg ccttgccctt cacgcgggcc
      661 gaatcgtcga catcggtcac cacgccggtc agccgcgcgc cggcgggcag cacgtcctgg
      721 ccgtcaatgg tgaccggacg cgtgagttcg gccgtgaccg cgtcctcgac agcgctcgtg
      781 tcggaggcga ccgccgtcat caaagccaac ggcagcgttg tgccaacggg aatcgtcacc
      841 gcacggtaga cgggacgatc cggcggttgc ctcgccgctc cagggggggt ggcgttccca
      901 ccgggtgtcg tgtcggtcgc gtccgacgcc gtcgtgccgc acgcagcgac gccaagcgcg
      961 gcgccaaccg tcaggatcag tagtcgcttc acattcacac tcctctgctc acttcgtatg
     1021 ctcacttcgt agagcagcgt aatcgctcga acgcgagacg tctgtgccgt tctgaacgtt
     1081 tcggttggtc gaactcacgt gtcccttctt gcacagacac gctcggtgcg accgggctat
     1141 cgtgaacctc gttcctgggc gcacggtgtg cccggagatg cgggattcca caatggacat
     1201 cccggctcat cccaaccccg ccgagtctgc gccgcggaca gccctgacct cctgcgaccg
     1261 ccacaagggc gacatcgaga tcctggacga gtttgcagcc gaactctgga gggcaggcgc
     1321 gcacgtgcgc ggcgggcgtg gatgcgactc aaggaaggat tcgacacatg ctgaccagaa
     1381 tcgttgcctc ggcgctcgcc gtgctcacgt tgggcattgg cggcacggcg caagcgcagc
     1441 cggccgacta tcgaacctat ttcacgttca gtgcaccggt gacattgcca ggtgtcactc
     1501 ttccggccgg cagatatctg ttccggctgg ccgatcccag cacgagccga aaggtcatca
     1561 acgtgatgag cgcggatggc aggacgtccc tggccatgct cctcacgatt cccaacaagt
     1621 tggcgaaggc gccggagaac gccgagattc gtttcatgga aaccccggcc aacactccgt
     1681 cggcgatcaa gacctggtgg tacccgggaa attcgatcgg gtatgagttc atctatccac
     1741 ggcagcaggc gctcctgctg gcgaaggtga actcagaacc ggtgttgacg acggtcgccg
     1801 agacgcgaga tctcgagaaa gccgaactgg ctcgcatctc cagagaaggt gttcccgccc
     1861 ccgtggtcgt ggacgagaac cctcccctgg tggtggccac cggcagagtc cagcagggtg
     1921 aggtggtgac cggcggcggc accgtgacct cttccgtgca agacccgcgc gccgatgagc
     1981 gcgcgcgcac gacgcggacc gcgctgccgc agacggccag ctcgttgcca accgtgttgc
     2041 tcttcggcac gttggcgatg ttggcgggtc tcggcctttc gctccggcgg cgcgccagcg
     2101 tttacgcgga ctgacggata cttgtgaggg gtgcggcgca ttccgcactc ctcacgctgc
     2161 cattcatatg tgctggccat gtgtcgcggt cgtcacggtg gcgatactgc tgctgcccgg
     2221 ctgcgtcaac gttcggggtc aggggccgcc ggtcttttcg gcagactcgg agctcgtggt
     2281 tctgcacgtg accgtcaaag acagaacagg tgcgtacgtc acaggcctgc cgcaggaggc
     2341 gttcagcatc gtcgaagatg gccgcgcaca aacggtccgc ctcttcatcg acatcgacac
     2401 gccggtcacc gtcggcttgc tcatcgacag cagcgcgagc atgcatccgc accggcgcct
     2461 ggtcattacg ggcgccacct cgttcgcgga agccagccat cctcgagacg aaatcttcgc
     2521 gctcgccttc aacgaggacg tccaccctgt gctctcaccg accgcgccat tcacgggcaa
     2581 cagcacggtg cttcacgcgg cactcgagcg cagcgtcacc gcccgcggac gcacggcgct
     2641 ctacgacgcg atttcggccg gactcgacta cctgtcacgt ggcactcgcg agcgcaaggt
     2701 gctcgtcgtg ctgagcgatg gcggggataa cgcgagccgg gcgacgcggg aggaatctgt
     2761 tcggagggca ctggcgtcga acgccgtcat ctacacgatt gggctggtcg cccttggcga
     2821 aagcgacgcg aatccgacgc ttctcaaaga gttggcgcag gcgagcggcg gcgaatcgtt
     2881 ccggccgcag gctcccaggg aaatcgcggc agtcctgggc cagatctccc gcaacatccg
     2941 ccatacctat acagtcggct acacgccgac caacaccgcg cgtgacggcg ccttccggcg
     3001 cgtccgcgtc gtggtgacgg cgcctcccgg ccggccacgg ccactcgcgg tcagaagccg
     3061 ggccggttat gtggccggaa ccacaaaggt cgaaccgtga cccgactcgt ccaacgctgg
     3121 ctcgcccgat tgctgatcgc cggcggcgcc atggccctga tgtgggtggc ggccaatctg
     3181 gtgtggacgg tgtcggttcg acgccagtac gaagcgacgc tcgagcgtat acgctcggca
     3241 ccggcagcag aggaaccggt cgcgccggta tcgctcccgg taggcgagcc catcggaacg
     3301 ctggagattg cgcgcatagg tctgtccggc gtcgtggtcg aaggagacga cgattcggtc
     3361 ctggatcgcg ccatcgggca cctgccggat actccgctcc catggcacac cgggaacagc
     3421 gcgctggccg cgcaccgaga caccttgttc aggccgttga ggggagttcg tcccggagac
     3481 gtcctgcgac tcaagacccc gcacggcgac ttcgactacg tcgttcgaga aaccgtgatc
     3541 gtcaagccgg acgatctgtg ggtgctcgac cccacgcctg tgtcgatgct cacgttgatc
     3601 agttgctatc cgttcaactt catcggcaac gcccccaagc gattcatcgt gcgtgccgag
     3661 cggatgccgc gcttcctcta gcggtcccag tcgcgggtcg tcctcgcggg catggtcaat
     3721 caggaactcg cgagaacgtg ttcgccaaat tgcaatccgc atattgatac ggtaacgtcg
     3781 ccgtcaggca tgacgcgcat cgacggccgg ctgatcgttt tcgtcgcgat gctcgcggga
     3841 ggcggcgtac tgctcggcgg tcagacgccg caaacgccgc ccgatcagca gtctccgcta
     3901 cccactttcc gcgcggccgt agaagccgtg caactcagcg tcatcgtcac cgacggcgac
     3961 ggcaaaccgg tcacaggcct gaccgcggct gatttcgaga tcttcgagaa ccgcgccccg
     4021 cgtgccatca cgacgttctc ggcggtcgac atcccggtcg agcggtccga gcgcgcgctc
     4081 gccgatccgg acgtgctcgg caacgaccgg ccgccgggac gactgtatgt gatcgcgttc
     4141 gacgcgatta caccggtgaa cgcgctgcgc gcgcgtgtgt tcctgcggca gttcatcgag
     4201 aactacttcg ggccgaacga tacggcggcg gtggtgctga ccacgcgcgg gccgcgcgat
     4261 tcagggcagg acttcacgag caacccgcgc ctgctgctga acgccatcga caggttcggc
     4321 ggcgaccagc ccgatgggtg ggattccacc atgcgggaac gcaacttcat cggcgacttc
     4381 cggaacctca tggccttcat ggcgacgctc cccggcgcgc gcaaggcgat gatttacgtg
     4441 acagagagca ttccggtgga cgcgtacgac gtcgtggatg cccg
//
LOCUS       DBNGSH010001005         3829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2690972, whole genome shotgun sequence.
ACCESSION   DBNGSH010001005 DBNGSH010000000
VERSION     DBNGSH010001005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2690972"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC16CY_22890"
     CDS             complement(<1..235)
                     /locus_tag="AC16CY_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356718.1"
                     /translation="MRTLLLIIAVSVVSLNAQSNPPLLLQRPTLSRTHIAFVFAGDLW
                     TVPREGGDARRLTTGVGVESNPVYSPDGSTIAFT"
     gene            complement(317..2725)
                     /locus_tag="AC16CY_22895"
     CDS             complement(317..2725)
                     /locus_tag="AC16CY_22895"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356719.1"
                     /translation="MMHRLDTIARDIRYACRSLARRPGFASVAVLTLAIGIGTMTVAF
                     SAVNAFFLARPPIDTAGAGLIEIDDGSPEGGGASFRELETFARDVPALDVSAQVVVTL
                     SRRAGGSAAIAWGLAVTDNYFDALGVKAALGRTFDEIDELSTVVSDRFWREELAEASL
                     TGLTVRLNGLDVPVVGVLPGDFRAGLYDAQVWVRIDDWDALRLPARSRRPDAFTLNLF
                     ARLRPGASPALANSQLQTASRELATAWPVIYAGRSASFVSYEEVGGTERRALAVVAMM
                     AMAMIGIVLLIALFNVVGLQLSRAVDREREMTLRGALGASRGRLTQQIVTESVVIAIV
                     GGTLALFVSRWSNTLLATFALETPLPQRIDVTPDWTVAAFTSALMIVCGIGAGLLPAR
                     RATSLAIATAMAPPTVVGGARSGRLRAAIVSMQVAGATLLLTMAALLVHSALTRASVP
                     LGFETARAIVLEIDPASHGYREPAAERFVTDALARLRALPGAVSAAVMDRVPFYVGFP
                     ARLEVSVDGRSCAVEMCPTAASYRVGPDYFRTMNIPLRRGRELDGSQGEQSAVISETM
                     ARSFWPAADPLGQWMSIGPEERRVQVVGVAADVTHRAAGERPEPYVYLPFEQSAYAEP
                     LAIVLRTRGDPQPLLQPASDQIRAMDSSLPIYQLRTMQQRIDARQQAGNLIVVKFFGI
                     CGGLALFLSIVGLSGTLSYAVSQRVREFGIRAAVGASPADQTRLVLGFTLRMAGPGIA
                     MGLFGALLLSWLFAARASGLNLDSPITFALVGLLQLTIAIIAAAIPGRRAARVTPLTA
                     LRES"
     gene            complement(3009..>3829)
                     /locus_tag="AC16CY_22900"
     CDS             complement(3009..>3829)
                     /locus_tag="AC16CY_22900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356720.1"
                     /translation="VEKILAARDENLQFDDPIVHCLPEGPRFSHFVAFPKKIVQTPTL
                     ILVLGEDLTFRQIFMDGRPLPTDPSPSFMGYSIGRWDGDTLVVESTGFKDRTWLDWAG
                     HPHSEKLHLSERWRRLDFGRLEIEETFTDPEYYNQPLTAKVTGTLVPDTDLLEYVCAE
                     NERDRPKLIGTSAEVKKQFQPVKVEPTVLAQYVGTYDFRFPENPTIPSMMQVALNGDT
                     LFLGSVPLVPIGEARFLLGPNPMEFVKDEQGRVTHFSATFVEGDLIGRRITEAK"
BASE COUNT          705 a         1265 c         1279 g          580 t
ORIGIN      
        1 ccgtgaacgc gatggtggag ccgtccggtg aatacaccgg gttgctctcc acgcccacgc
       61 ccgtggtcag gcgccgggca tcgcctccct cgcgcggcac ggtccacaag tcgccggcaa
      121 agacaaaggc gatgtgcgtc cggctcaacg tgggtctctg cagcagcagc ggtggattcg
      181 actgcgcgtt cagggagacg accgacacgg cgatgatcag aagcagcgtg cgcattggag
      241 agcctccgcc ggaagcataa cccagggcgc gaacacggcc ccaggcgacc gtgaaactgc
      301 aatggcagaa ggccggttac gactcgcgca gcgcggtgag gggcgtcaca cgcgcggcac
      361 gccggccggg aatcgccgcg gcgatgatcg cgatggtcaa ctgcaggagc ccgacgagcg
      421 cgaacgtgat cgggctgtcg agattcaacc cgctcgcgcg cgcggcgaac agccatgaga
      481 gcagcagggc gccgaagagg cccatcgcaa tgcccggccc ggccatgcga agggtgaagc
      541 caaggacgag ccgtgtctgg tcggcaggag atgcgccgac agccgcgcgt atgccgaact
      601 cgcggacgcg ctggctgacg gcatacgaca gcgtgccgct cagcccgacg atcgacagga
      661 acagcgcgag cccgccgcag atgccgaaga acttgacgac gatcaggttg ccggcctgct
      721 gacgcgcgtc gatacgctgc tgcatcgtcc gcaactggta gatgggcagc gaagaatcca
      781 tggcccgaat ctgatcagac gccggctgca gaagcggctg cggatccccg cgagttctca
      841 gcacgatcgc cagcggctct gcgtacgcgg attgctcgaa cggcaggtag acgtacggct
      901 cgggacgctc gccggcggcg cggtgcgtca catcggcggc cacgccaacc acttgcacac
      961 gccgctcctc cgggccgatt gacatccact gtcccagggg atcggccgcc ggccagaagc
     1021 tcctggccat cgtttcgctg atcaccgccg actgctctcc ctgcgacccg tcgagctcac
     1081 gtcctcgccg cagcgggatg ttcatggttc ggaagtaatc aggacccacg cgatagctgg
     1141 ccgcggtggg acacatctcg acagcgcacg aacggccgtc caccgacacc tcgagccgcg
     1201 cgggaaaccc gacgtagaac ggcacgcgat ccatgacggc ggcgctcacc gcgccaggca
     1261 gggcacgcag gcgtgccagg gcgtcagtga caaatcgctc cgccgccggt tcccggtagc
     1321 catgggaagc cggatcgatc tcgagcacta tcgcccgggc ggtctcgaag ccgaggggaa
     1381 cggacgccct cgtcaaggca ctgtgcacga gcagtgcggc catcgtcagc agcagggtgg
     1441 caccggccac ctgcatcgac acgatcgcgg ctcgcagtct gccggaacga gctccaccga
     1501 caacggtcgg cggcgccatc gcggttgcga tcgcgaggct cgtggctcgc cgcgcgggca
     1561 agagcccggc cccgatgccg cagacgatca tcaacgcgct cgtgaacgcc gcaacggtcc
     1621 agtcgggcgt gacgtcaatg cgttgcggca acggcgtttc gagcgcaaag gtcgccagca
     1681 gggtgttgct ccacctcgag acgaagagcg cgagcgtgcc cccgacgatg gcgatcacga
     1741 cgctctcggt cacgatctgc tgtgtcaatc gaccccggct cgcgcccagc gcgccgcgga
     1801 gcgtcatctc ccgctcgcga tcgacggcgc gcgacaactg caggccgacg acgttgaaga
     1861 gcgcgatcag gagcacgatg ccgatcatcg ccatcgccat catggccacg acggcaagcg
     1921 cacgccgttc cgtgccgcca acctcttcgt atgacacgaa cgacgcagag cggccggcgt
     1981 agatgaccgg ccacgccgtg gccagctcgc gggaagccgt ctgcagctgg ctgttcgcaa
     2041 gtgccggaga ggctccgggc cgcaggcgcg caaacaggtt cagcgtgaac gcgtcggggc
     2101 ggcgactgcg ggccggcaat cgaagcgcgt cccagtcgtc gatgcgaacc cagacctgcg
     2161 cgtcgtagag cccggcgcgg aaatcgcccg gcagcacgcc gacgacggga acgtcgaggc
     2221 cgttgagacg taccgtgagg cccgtgaggg aggcttcggc gagttcttcg cgccagaatc
     2281 gatcgctgac aacggttgac agttcgtcga tctcgtcgaa ggtccgcccg agcgccgcct
     2341 tgacgccgag tgcgtcaaag tagttgtccg tgaccgccag tccccaggcg atcgctgcgc
     2401 tgcccccggc acgccgactc agtgtgacaa cgacttgcgc gctgacgtcg agcgccggca
     2461 cgtcgcgcgc gaacgtctcg agctcgcgga aagacgcgcc gccgccttca ggagagccgt
     2521 catcgatctc gatcaggccc gcgccagccg tgtcgatggg aggtctcgcg aggaagaagg
     2581 cgttcacggc gctgaacgcc accgtcatcg tgccgatgcc gattgccagc gtcagtacgg
     2641 cgacgctggc aaatcccggg cggcgggcca gcgaccggca ggcgtagcgg atgtcgcgcg
     2701 cgatcgtgtc caggcggtgc atcatggtcc ggcctcggtg gatggggcat cagcgggtcg
     2761 gtcccgcagt caacgatgag ggtgaggggg cacgatggag aggcgattct acgctacaac
     2821 ccgctctaca actcgaactc gccgaagctc gtctcaggcg ctcgccgcgc ggcaacaccg
     2881 tggcgatgtc gccgtgcacg ccgccgtgga tcttcgaata gatcctcgat gagcaccgag
     2941 tcgtacgaat cgggcgcccc gaggtcggcc tcgaggcgca acgtgatctg cgtcgcggga
     3001 ggagtcgact acttcgcctc cgtgattctc cgtccgatca gatcgccttc gacgaatgtc
     3061 gctgagaagt gcgtgacgcg gccctgctcg tccttcacga actccatcgg attggggccg
     3121 agcagaaaac gcgcctcgcc gatcggcacc agcgggacac ttccgaggaa gagcgtgtcg
     3181 ccgttgagcg ccacctgcat catcgaagga atcgtcgggt tttccgggaa gcggaagtcg
     3241 taggtcccga cgtattgagc gagcacggtt ggctcgactt tcaccggctg gaactgtttc
     3301 ttcacttcag ccgatgttcc gatcagcttt ggccggtccc gctcgttctc ggcgcacacg
     3361 tattcgagaa ggtccgtatc cggcacgagc gtgcccgtga ccttcgcggt caacggctgg
     3421 ttgtagtact cgggatccgt gaacgtctcc tcaatctcga ggcggccgaa gtccagccgg
     3481 cgccagcgtt cgctgagatg gagcttctcg ctgtgcggat gcccggccca gtcaagccag
     3541 gtgcggtcct tgaaaccggt ggactcgacg acgagcgtgt cgccgtccca tcgaccgatg
     3601 gaatagccca tgaaactcgg gctcggatcc gtcggcagcg gacggccatc catgaagatc
     3661 tgccgaaagg tcagatcctc tccgagcacg agaatgagcg tcggcgtctg gacgatcttc
     3721 ttcgggaacg cgacaaagtg gctgaagcgc ggaccctctg gcaggcagtg cacgatcgga
     3781 tcgtcgaact gcaggttttc gtcgcgcgcc gccaggatct tctcgactg
//
LOCUS       DBNGSH010001006         3749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4559627, whole genome shotgun sequence.
ACCESSION   DBNGSH010001006 DBNGSH010000000
VERSION     DBNGSH010001006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4559627"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="AC16CY_22905"
     CDS             <1..350
                     /locus_tag="AC16CY_22905"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239058.1"
                     /note="AcsA; in Sinorhizobium meliloti this enzyme is
                     required for acetoacetate activation; similar to
                     acetyl-CoA synthase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR3356721.1"
                     /translation="AVEDSLIVNLDLPGGGFFMPLFVKTRNNVTLDESIGDEIRARIR
                     RDYSPRHVPDKIVQVTAIPYTLTGKKMEVPVRRILMGVPVEKAANRAAMANVESLDYF
                     IEYARTQRDYSLT"
     gene            387..1196
                     /locus_tag="AC16CY_22910"
     CDS             387..1196
                     /locus_tag="AC16CY_22910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3356722.1"
                     /translation="MRHLILAVLAAASLASQQSFSFEVASVKPARPDNPQGFQGVSRP
                     PGGRFNAVNAPLRFLILYAYQLQGYQLIGAPEWIADERYDVVARMEGDAPSIVTSGVD
                     PIRLALRTLLADRFGLVAHRETRELDIYALTMAKPGGQPGRGLKASPEDCAVMAARAK
                     AAPPATDADGFPPFICGQSWGQGRIRFAGYPLSMFANGLSQNVGRAVVDRTGLTGNWT
                     FELTYAAEPGPDSDAPSLFTALQEELGLKLESTKGPVEVLVIDSVRPPTPD"
     gene            1253..1720
                     /locus_tag="AC16CY_22915"
     CDS             1253..1720
                     /locus_tag="AC16CY_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356723.1"
                     /translation="MKAIVLAALVVLAPCVAAAQGLAGLEGRWLGSCPGCAAGAMPGG
                     FARTLVIRVSTSAITIQQGGLSAEAYQLDGSDTQLRDGRTATALVADGSLVVRTVRKR
                     ARSRDEVFQTILRDIYRVSGNTLTVERSARAIRPDEPPSEKWASVGEVVYRRD"
     gene            complement(1746..2366)
                     /locus_tag="AC16CY_22920"
     CDS             complement(1746..2366)
                     /locus_tag="AC16CY_22920"
                     /inference="COORDINATES: protein motif:HMM:NF043005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCE4755 family polysaccharide monooxygenase-like
                     protein"
                     /protein_id="HIR3356724.1"
                     /translation="MKSRILFGVIAAASLSGVADAHFVLIQPAASLIQNRLGDPQKIG
                     PCGGVSANPGRGTPPNPGVPSGAFTNVKGGTDLPLLVHESIFHPGHYRVALARTMAQL
                     PKDPEVTVVETEKGPRSKSAVIQNPPAPPVLLDGIFAHTEKPASPQTFDTMIPIPNIN
                     CPSCVLQVIEFMADHPGIAVDGGHNYHHCAILNITADPDKPIDKRW"
     gene            complement(2412..3713)
                     /locus_tag="AC16CY_22925"
     CDS             complement(2412..3713)
                     /locus_tag="AC16CY_22925"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3356725.1"
                     /translation="MRHSTPVKPRRLAWFALAAGGAVVIQLAGTAQQTVPFNNGTPVA
                     PAGFEPHAIPAEPIEYDTAEVMRIRVVPVARGLVNPWSVTFLPSTSTSPGVTMLVTEK
                     EGRVRVIRNDVLDPKPVPGAPVVRVQGRSGLMDVVLHPQFASNRLVYFSYLKPVGDER
                     QAALTVARGRFDGSAISELKDIFSCGPGVGGPSRLAWGRDGKLYVTTPSGGTGSGSQD
                     PNTYSGKVLRLNEDGTVPADNPFVGRAGHKPEVYTFGHRNSLGLAVHPATGEMWQNEM
                     GPNGGDEINILKPGANYGWPIVSLGRTYSGPWQSPTFSKEGIESPVVYWMPSISLSGM
                     AIYTGNKLPKWKGDVFVGGQRTGEIPGTGRLERILVNEKLEELRRESLLVPLGMRIRD
                     VRQGPDELLYVVVDHDTNGGILRIEPSATRQMPTTYSARPK"
BASE COUNT          630 a         1213 c         1231 g          675 t
ORIGIN      
        1 cggccgtcga agactcgctc atcgtcaatc tggatcttcc cggcggcggg ttcttcatgc
       61 cgctgttcgt caagaccagg aacaacgtga cgctggacga gagcatcggc gatgagatcc
      121 gcgcccgaat ccggcgcgac tactcgccgc gccacgtccc ggacaagatc gttcaggtca
      181 ccgccatccc ctatacgctc accggcaaga agatggaagt gccggtgcgc cgcatcctga
      241 tgggggttcc ggtggagaag gccgccaatc gcgctgcgat ggccaatgtc gagtcgctgg
      301 attacttcat cgagtacgca cgcacgcagc gcgactactc actcacgtag agtccggcta
      361 tagccggacc cggcattctg agagccatgc gacatctgat tctcgccgtc ctcgccgccg
      421 cgagcctcgc gtcacaacag tcgttctcgt tcgaggtcgc gtcggtgaag ccggccaggc
      481 cggacaatcc acagggcttc cagggcgtgt cgcgcccccc gggtggacgc ttcaacgccg
      541 tcaacgcgcc gcttcggttc ctcatcctgt acgcctatca gctccagggg tatcagctga
      601 ttggcgcgcc tgaatggatt gccgacgagc gctacgacgt cgtcgccagg atggagggcg
      661 atgcgccttc gatcgtgacc tctggcgtcg atccgattcg tctcgcgctg cgaacgttgc
      721 tcgccgatcg cttcgggctg gtcgcacatc gcgagacgcg tgagctcgac atctacgccc
      781 tgaccatggc aaagccggga ggacagccgg gtcgcgggct caaggcgtcg cctgaagact
      841 gcgcggtgat ggcggcgcgc gcgaaggcgg ccccgcccgc gacggacgcc gacggctttc
      901 cgccgttcat ctgcgggcag tcgtgggggc agggacgcat ccgctttgcc ggctatccgc
      961 tctcgatgtt tgcgaacggc ctgtcgcaga atgtcggtcg agcggtcgtc gatcgtaccg
     1021 gcctcacagg caactggacg ttcgagctga cctacgccgc cgagcccggg ccagactcgg
     1081 acgcgccgag cctgttcacc gcgctacagg aggaactggg attgaagctc gaatcgacga
     1141 aagggcccgt cgaggtcctg gtaatcgaca gcgtgcggcc gcccactccg gactaagggt
     1201 cagtaataaa gcggtaacgt cctttcgtga cacttcccgg agaggtgcaa ccatgaaggc
     1261 gatcgtgctc gctgctctcg tggtcttggc gccatgcgtg gcggcagcac aagggctcgc
     1321 cggtctcgag ggacgctggc tcggctcctg ccccggctgc gcggccggcg cgatgccggg
     1381 cggatttgcg aggactctgg tcatccgggt ctccacatcg gcgatcacga ttcaacaggg
     1441 cggattgtcg gccgaggcgt accagctcga tgggagcgac acccaactcc gcgatgggag
     1501 aacggcaacg gcgctggtcg cggacggcag cctggtggtg aggacggtac ggaagcgagc
     1561 gcggagtcgt gacgaggtat tccagacaat ccttcgcgac atctatcgcg tctccgggaa
     1621 cacgctgacc gtcgagcgct ctgcacgcgc aatccgcccg gacgagcctc cttcagagaa
     1681 gtgggcatcg gtcggggaag tcgtctatcg gcgtgactga ggcgacgcgc ctcgctctga
     1741 tcgcttcacc accgcttgtc gatcggcttg tccggatccg cggtgatgtt gagaatcgcg
     1801 cagtggtgat agttgtgacc gccgtccacg gcgatgccgg ggtggtcagc catgaactcg
     1861 atcacctgca gcacgcaact cgggcagttg atgttcggga tcggaatcat ggtatcgaag
     1921 gtctgcggcg aggccggttt ctcggtgtga gcgaagatcc cgtccagcag gacgggcgga
     1981 gcgggcggat tctgaatgac cgccgacttc gaccgcggcc ctttctcggt ctccacgacc
     2041 gtcacctcgg ggtccttcgg cagctgcgcc atcgttctcg ccagcgccac gcgatagtgc
     2101 cctggatgga agatcgattc gtggacgagc agcgggagat cggtaccacc cttcacgttc
     2161 gtgaaggcgc cgctcggcac gcctggattc ggcggcgttc cacgtccggg attcgccgac
     2221 acgccaccgc acggcccaat cttctgtgga tcgccgagcc ggttctgaat cagcgaagca
     2281 gccggctgga tgagcacgaa gtgcgcgtcg gccacgcctg acaggctggc ggctgcgatc
     2341 acaccaaaca ggattctgct cttcatggcc gacaattgta gtcgtccgac tccggctttg
     2401 gcgatagagg cctacttcgg ccgcgccgag taggtcgttg gcatctggcg tgtcgccgac
     2461 ggctcgatcc gcaggatgcc gccattcgtg tcgtgatcga ccaccacgta cagcagttca
     2521 tcggggccct gccggacgtc gcggatgcgc atcccgagcg gaacgagcag cgactcgcgg
     2581 cggagctctt cgagtttctc gttgacgagg atccgctcga ggcggccggt accggggatt
     2641 tcgccggtcc gctgtccgcc cacaaagaca tcgcccttcc acttcggcag cttgttgccc
     2701 gtatagatgg ccatgccgga cagggaaatg gacggcatcc agtacacgac cggcgattcg
     2761 atgccctcct tcgagaacgt cggcgactgc cacggtcccg aataggttct gccgaggctc
     2821 acgatcggcc agccgtagtt ggcgccgggc ttgaggatgt tgatctcgtc gccgccgttg
     2881 ggccccatct cgttctgcca catctccccg gtcgccggat gaacggcgag tcccagcgag
     2941 ttgcggtgcc cgaaggtgta gacctcgggc ttgtgtccgg cgcgaccgac aaacgggttg
     3001 tccgcgggca ccgtgccgtc ctcgttcagg cgaagcacct tgcctgagta cgtgttcgga
     3061 tcctgtgagc cgctgccggt cccgccgctt ggcgtcgtca cgtagagctt gccgtcgcgc
     3121 ccccaggcga gccgcgacgg accacccact ccgggtccgc agctgaagat gtccttgagc
     3181 tcggagatgg ccgacccgtc gaagcgcccg cgagcgaccg tcaatgcggc ctgccgctcg
     3241 tcgccaaccg gcttcagata cgagaagtag acgagtcgat tgcttgcgaa ctgcgggtgc
     3301 agcacgacgt ccatgagccc cgagcggccc tgaacccgaa cgaccggcgc gccgggaaca
     3361 ggtttcgggt cgagcacatc gtttcgaatg actcgcaccc gcccttcctt ctcggtcacc
     3421 agcatcgtca cgccgggcga agtcgaggtg cttgggagga acgtcacgct ccacggattc
     3481 acgagcccgc gggccaccgg gacgacgcgg atccgcatga cttcggccgt gtcgtattcg
     3541 atcggttcgg ccgggatcgc gtgcggctcg aatccggccg gtgccaccgg cgtcccgttg
     3601 ttgaaaggca ccgtctgttg cgccgtgccg gcaagttgga tgacgaccgc gccgccggcg
     3661 gcaagcgcga accaggcgag acggcgtggc tttactgggg ttgagtgccg catgggcgaa
     3721 tctcctcgca agcggcatca gtatacggc
//
LOCUS       DBNGSH010001007         4166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1843140, whole genome shotgun sequence.
ACCESSION   DBNGSH010001007 DBNGSH010000000
VERSION     DBNGSH010001007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1843140"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="AC16CY_22930"
     CDS             <1..279
                     /locus_tag="AC16CY_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356726.1"
                     /translation="TTGLHFDDIAKLLAAFRKLVEAGHTLLVIEHNLDVVKTADYLID
                     LGPEGGDAGGRVVAAGTPEQVVQVEESHTGRALRDVLAAGRSNAYAAR"
     gene            344..970
                     /locus_tag="AC16CY_22935"
     CDS             344..970
                     /locus_tag="AC16CY_22935"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR3356727.1"
                     /translation="MTPDPLTKSFYERPTLTVAAELIGKVLVHRTRAGAAAGMIVEVE
                     AYIGEDDPACHAAPGLTTRNAPLYGPPGHAYVYLNYGVHRLVNAVTEAEGSPAAVLIR
                     ALEPIDGVDLMVERRSRGRARLDERDLCRGPGNLTQALGITLDQNRADLVAGPLRIED
                     RRFTAGTICWGPRIGIRRGVDRQWRCWVKGHPCVSGPRATMKVRHAND"
     gene            970..2373
                     /gene="leuC"
                     /locus_tag="AC16CY_22940"
     CDS             970..2373
                     /gene="leuC"
                     /locus_tag="AC16CY_22940"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3356728.1"
                     /translation="MGTTLLKKVWDLHTVRPLPTGQTQLFIGLHLVHEVTTPQAFDEL
                     RARGWKVARPDLTFATADHIVPTMARTRPFLDVMAEDMMASLERNVRDFGLRFAGLED
                     ERQGIVHVIGPELGLTQPGMTIACGDSHTSTHGAFGAVAFGIGTSQVRDVLASQCLAM
                     EPLKVRRIRLTGALPRGVYAKDVILTIIQRLGVRGGTGYAYEYAGDTVERMSMDERMT
                     ICNMSIEGGARVGYVNPDETTFAYMRGRPFAPQGDAFERARTWWTSMASDPDAAYDDE
                     VEIRAAELTPVVTWGVNPGQSIGIDGSVPSPSEPGVEPQAVSDALKYMGLKPGQPIAG
                     TKVDVAFIGSCTNGRLSDLREAARVVRGRRVAPRVKALVVPGSVSISRAAEREGLDHI
                     FREAGFEWRGAGCSMCLGMNPDKLEGNQVCASSSNRNFKGRQGSPTGRTLLMSPAMVA
                     AAAVSGEVIDVREMVDR"
     gene            2370..2972
                     /locus_tag="AC16CY_22945"
     CDS             2370..2972
                     /locus_tag="AC16CY_22945"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3356729.1"
                     /translation="MTRITRIDGRGLPLRGSDIDTDRIIPARYLRSITFEGMEQHLFE
                     DDRKTTAHPLDDARFAGASVLIVNSNFGCGSSREHAPQAIHRWGIDAVVGESFAEIFF
                     GNSLMIGMPCLTASGADVRALMDLVERDPRTEIRLDLASGTCEAGDFRCAVSLPPNCR
                     DAFATGAWDTTGMLLDRYDEVDATAAHLPYIRGFPVEAGL"
     gene            3066..3806
                     /locus_tag="AC16CY_22950"
     CDS             3066..3806
                     /locus_tag="AC16CY_22950"
                     /inference="COORDINATES: protein motif:HMM:NF019032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR3356730.1"
                     /translation="MAEASPVRRFALVAFALAILAIAATVYAIAGSRNGLVSYINAFT
                     WLRWIAVGSTALIGPAVLIWLVSMRLPDTGATVYSGVATLVLFALSGTLIVNRAEPPP
                     GPFINDITTDLEDPPRFEAVIPLRPAGSTPAEYGGAHTATNQRQVHPDVRPIDTTLTP
                     PAAFARALSLAQEMGWNIVAANESTGVIEAVDTTTFFRFKDDIVIRVRPAGAGSRVDL
                     RSHSREGLTDLGKNAARIREYSAAFSGH"
BASE COUNT          696 a         1372 c         1432 g          666 t
ORIGIN      
        1 accacgggcc tgcatttcga cgacatcgcg aagctgctcg ccgccttccg gaagctcgtg
       61 gaggccgggc acacgctgct cgtcatcgag cacaatctcg acgtcgtcaa gacggcggac
      121 tacctgatcg atctcggacc cgaaggtggt gatgccggcg gacgcgtcgt cgccgccggg
      181 actcccgagc aggtggttca ggtcgaagag tcgcacaccg gccgcgcgct tcgcgacgtg
      241 ctcgcggccg gacggtcgaa tgcgtatgcc gcgcggtaaa gggggtcggg agtcatttct
      301 tgaggacacc cgctatgagt ctgcggagaa gtgtcctcaa gaaatgactc ccgaccccct
      361 tacaaaatcg ttctacgaga gaccgacgct caccgtggct gccgagttga tcggcaaggt
      421 cctcgtccac cgcacgaggg ccggcgccgc cgcggggatg atcgtcgaag tggaggccta
      481 cattggcgaa gacgacccgg cctgccatgc tgcgcccggt ctcacgacgc ggaacgcgcc
      541 gctctatggc ccgcccggac acgcgtacgt gtatctgaac tacggcgttc accggctcgt
      601 gaacgcggtg accgaagccg agggctcgcc cgccgcggtg ctgatccgcg cgctcgagcc
      661 gattgacggc gtcgatctga tggtcgagcg gcggtcgcgc ggccgggccc ggctggacga
      721 gagggacctc tgtcgggggc cgggcaacct gacgcaggcg ctcggcatca cgctcgatca
      781 gaatcgcgcg gacctcgtcg cgggcccgct ccggatcgag gaccgccggt tcactgcggg
      841 cacgatttgc tggggtccga gaatcggcat ccggcgcggc gtcgatcgac aatggcggtg
      901 ctgggtcaaa ggccatccgt gcgtctcggg gccgcgtgct acgatgaaag ttcgccacgc
      961 caacgactga tgggaacgac gcttctgaag aaagtgtggg atctccacac cgtccgcccg
     1021 cttccaaccg ggcagacgca gctgttcatc ggcctgcacc tcgtgcacga ggtcacgacg
     1081 ccgcaggcct tcgacgagct gcgggcgcgg ggttggaagg tggcgcgtcc ggatctgacg
     1141 tttgccaccg ccgatcacat cgtgccgacc atggcgcgga cgcgcccgtt tctcgacgtc
     1201 atggccgaag acatgatggc ctcgctcgag cgcaacgtcc gcgacttcgg tctccgcttc
     1261 gccgggctcg aggacgagcg ccagggcatc gtgcacgtca tcggcccgga gctcgggctc
     1321 acgcagcccg gcatgacgat cgcgtgcggc gacagccaca cctcgacgca cggggcgttc
     1381 ggcgcggtgg cgttcggcat cggcacgtcg caggtaaggg acgtcctcgc gtcgcagtgc
     1441 ctggccatgg agccgctcaa ggtccggcgc atccgcctga ccggagcgct gccgcggggc
     1501 gtctatgcga aggacgtcat cctcaccatc atccagagac ttggcgtgcg cgggggcacg
     1561 ggttacgcct acgaatacgc gggcgacacc gtcgagcgca tgtcgatgga cgagcgcatg
     1621 acgatctgca acatgtcgat cgagggcggc gcccgcgtcg gctacgtcaa cccggacgag
     1681 acgacgttcg cctacatgcg cggtcgtccg tttgctccgc agggggacgc cttcgagcgc
     1741 gccagaacct ggtggacgtc gatggcatcc gatccggacg cggcctacga cgacgaggtg
     1801 gagatccgcg cggcggagtt gacgccggtc gtcacctggg gcgtgaaccc cggccagtcg
     1861 attggcatcg acggcagcgt gccgtccccc agcgagcccg gcgtcgagcc gcaggcggtg
     1921 tcggacgcgc tgaagtacat ggggctcaag ccgggccagc cgattgccgg aacgaaagtt
     1981 gacgtggcct tcatcggatc gtgcaccaac gggcgcttgt ccgatctccg cgaagcggcg
     2041 cgtgtggtgc gcggccgccg cgtggctccg agggtgaaag cgctcgtcgt tccggggtcg
     2101 gtctcgatca gccgcgcggc cgagcgcgaa ggcctcgacc atatcttcag ggaggcgggg
     2161 ttcgaatggc gaggggccgg gtgctcgatg tgcctcggga tgaaccccga caagctcgaa
     2221 ggcaaccagg tgtgcgcctc ctcctcgaac cgcaacttca aggggcggca gggaagtccg
     2281 accggccgaa cgctcctcat gagcccggcg atggtggcgg ccgccgcggt ctcgggcgag
     2341 gtgatcgacg tgcgggagat ggtggaccga tgacccggat cacgcggatc gacgggcgcg
     2401 ggctgccgct gcggggcagc gacatcgaca ccgatcgcat catccccgcg cggtacctcc
     2461 gttcgatcac gttcgagggg atggagcagc acctcttcga ggacgaccgg aagacgacgg
     2521 cacacccgct ggacgatgcg cgcttcgcgg gagcgtcggt gctcatcgtc aactcgaact
     2581 tcgggtgcgg atcgtcgcgg gagcacgcgc cgcaggccat tcaccggtgg gggatcgacg
     2641 cggtcgtggg cgagtccttc gcggagatct tcttcggcaa ctcgctcatg atcgggatgc
     2701 cgtgcctgac cgcgtccggc gccgacgtcc gcgcgctcat ggatctggtc gaacgcgatc
     2761 cgcggaccga gattcgcctc gacctcgcct cgggcacctg cgaggccgga gacttccgct
     2821 gcgccgtctc gctgccaccc aattgccgcg acgcgtttgc aaccggcgcc tgggacacga
     2881 cgggcatgct cctggatcgc tacgacgagg ttgacgccac ggcggcgcac ctgccttaca
     2941 tcagaggctt tcccgtagag gcggggcttt agacccgcct tgtgagtaga ggcgggtctt
     3001 tagctttctc gtagaggcgg gtctttagac ccgccttctc agccttccga gcaagcccga
     3061 gggacatggc cgaagccagc ccggttcgac gatttgcgct ggttgcgttc gcccttgcca
     3121 tcctggcgat cgccgcgacg gtgtacgcaa ttgcgggatc gcgaaatggc ctcgtctcgt
     3181 atatcaacgc gttcacctgg ctccggtgga ttgccgtcgg atcgaccgcg ctgatcgggc
     3241 cggccgtcct catctggctc gtctcgatgc ggctgcccga cacgggcgcg acggtctatt
     3301 caggcgtcgc gacgctcgtc ctgtttgcct tgagcggaac gctgatcgtg aaccgggccg
     3361 agccgccgcc gggtcccttc atcaacgaca tcacgacgga cctggaggac cctccgcgat
     3421 tcgaggcggt gattccgctg cgccccgccg gcagcacccc cgcggagtac ggaggcgcgc
     3481 acacggcgac caatcagcgg caggtgcacc cggacgtccg gccaatcgac acgacgctga
     3541 cgccgcccgc tgcgttcgca cgggcgctct ccctggccca ggagatgggg tggaatatcg
     3601 tggccgccaa cgagtccacg ggcgtcatcg aagcggtgga cacgacgacg ttcttcagat
     3661 tcaaggacga tatcgtgatt cgcgtgcggc cggccggcgc cggaagccgc gtggatctgc
     3721 gatcgcactc gcgggagggc ctcaccgatc tcggcaagaa cgccgcccgg atcagagagt
     3781 acagcgccgc gttctcaggc cactgaggtc cgtggtcctt ggtccctggt tccaggtccg
     3841 tccagagtcc ttcgtccgtc catggtcctg ggttcttggt ccaaccctcg gaccaaggac
     3901 cggggaccac ggacggacca aggaccgggg accacggacg gaccaaggac cggggacgga
     3961 ccaaggacca aggaacggac caaggaccaa ggaacggacc aagcacgaag gactaaggac
     4021 caagaaccag ggttaagccg gcttcttctt ctcctcgagc atcgcgagga aggcggcaga
     4081 ggcgtgagat cgctctccgc tcttgcggaa gatcagccgc acctggcgct tgatgcggac
     4141 ctcgggcatc gccagcgcga ccagct
//
LOCUS       DBNGSH010001008         3592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4051180, whole genome shotgun sequence.
ACCESSION   DBNGSH010001008 DBNGSH010000000
VERSION     DBNGSH010001008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4051180"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="AC16CY_22955"
     CDS             <1..1338
                     /locus_tag="AC16CY_22955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3356731.1"
                     /translation="RSYLDASVDGFVILFALIVTCGSAVMFGLVPALRSARLDLAASM
                     KDDLSPRGGSRGRLRAVLVVAQVAVSLLLLVGVGLVMRSVDAARHADGGFDQRDVAVV
                     SLDVTPGGYDEARGRVFYERLLDAVRAHPGVESASLAASMPLSLVDGPSQRISVERDV
                     PKQGEDTIFLFNRVADGYFDTMRIPLLAGRDFEDRDGPGSQQVVIVNETMAVRFWGGA
                     QQAVGKRLRTGDSDWKVVVGVARDIKYARVTEASRPHVYEPFDQRYAPARILHVRGRG
                     LDERTLIETARASVASVDPTMPILLSTMLREQTKLGLAVLEVAGGVLSVFGVIAMALA
                     ALGTYGLIAYAAKQSTHEIGIRMAVGASRGHVARRFLQRGLRLGAIGALLGLLAAGAL
                     TRLLAGLVYGVSVFDAVSFVSALAAVLLAVLTASSIPAWRASRTDPIAALRHR"
     gene            complement(1432..1965)
                     /locus_tag="AC16CY_22960"
     CDS             complement(1432..1965)
                     /locus_tag="AC16CY_22960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356732.1"
                     /translation="MRFPKVSLIVPMLGVGLAIPAITGAHGDDRPQYQFEQRTYERMR
                     GLAHVLDERAQHAVRQAIDGAHHGGRSEQRFLNDIRHFARRAADFHRRMDRYRQAPWN
                     VPGEIDHLTDDARQVDRRLRNAHVFEHTWDDWAAVIDVLAQMQRSAVVERGRDRYGDH
                     AGTRNGIRYSDRDRRRY"
     gene            2160..2726
                     /locus_tag="AC16CY_22965"
     CDS             2160..2726
                     /locus_tag="AC16CY_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HIR3356733.1"
                     /translation="MNACVLRTAYVGLCLLACLSMETRAIAQNAGAPAAQPAPPLTFA
                     FELRAAVGDPVEIGQIPHGRRRIVPILGGTVRGPMLTAAVVPGSGSDWQLIQPDGFSE
                     LDTRYTLRTEKGQLVYVQNAGIRHAAPEVMKKLLAGEVVDPKLVYFRTVPKFETSAPD
                     LQWLTRSVFVGVGERFPSEVVVRFYRLE"
     gene            complement(2801..3199)
                     /locus_tag="AC16CY_22970"
     CDS             complement(2801..3199)
                     /locus_tag="AC16CY_22970"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3356734.1"
                     /translation="MTKSISRFVVLFAFIMASASVYAHHSLAGVYALGKEAKVTGAFK
                     AFRLVNPHSTMKIDVKNKDGSVTEWTFLGGSVGQIARLGLKEGPNALKPGDEITVTIV
                     PAIDGKSPVGLLSAITYADGHTVRFRSPDE"
     gene            complement(3240..>3592)
                     /locus_tag="AC16CY_22975"
     CDS             complement(3240..>3592)
                     /locus_tag="AC16CY_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356735.1"
                     /translation="HTKEAEFDITYMGNTVGRWEGDTLVLEAIAFIDTTWLGRGGFFH
                     SDRMHVVERFTRRGDAILYDVTVEDPEVLVEPWVLPTRTLRRNTNPNGGLIRERGNCE
                     TSFETEAAATQIRH"
BASE COUNT          542 a         1184 c         1202 g          664 t
ORIGIN      
        1 cgaagctacc tcgacgccag cgtggacggg ttcgtgatcc tgttcgcgct gatcgtcacg
       61 tgcggcagcg ccgtgatgtt cggcctggtg cccgccctgc gcagcgcgcg tctcgatctc
      121 gcggcaagca tgaaggacga tctctcgccg cgcggcggct cgcgcggccg tctgcgcgcc
      181 gttcttgtcg tcgcccaggt ggccgtgtcg ctgctcctcc tcgtcggcgt cggcctcgtg
      241 atgcgcagcg tcgacgccgc gcggcacgcc gacggcgggt tcgatcagcg cgacgtcgcg
      301 gtggtgtcgc tcgacgtgac gccgggcggc tacgacgaag cgcgcggacg cgtgttctac
      361 gagcgtctgc tcgacgccgt gcgggcgcat ccaggggtcg agtcggcgag cctcgccgcc
      421 agtatgcctc tgtcgctggt cgatggtccg tcgcaacgaa tcagcgtcga gcgcgacgtg
      481 ccgaaacaag gggaggacac gatctttctc ttcaaccggg ttgccgacgg ctacttcgac
      541 acgatgcgca ttccgctcct ggccgggcgg gacttcgagg atcgggacgg gcccggcagc
      601 cagcaggtgg tcatcgtcaa cgagacgatg gccgtgcgct tctggggagg cgcgcagcag
      661 gcggtcggca agcgcctgcg cacgggcgac agcgactgga aggtggtcgt cggtgtggcg
      721 cgcgacatca agtacgcgcg ggtgaccgaa gcctcgcggc cgcacgtata cgagccgttc
      781 gaccagcggt acgcaccggc gcggatcctg cacgtgcgcg gacgaggcct ggacgagcgg
      841 acgctcatcg agacggctcg cgccagcgtc gcgtcggtgg atccgacgat gcccattctg
      901 ctgtccacga tgctccgtga gcagacgaag ctggggctgg ccgttctcga ggtcgcgggt
      961 ggcgtgctgt ccgtcttcgg cgtgatcgcg atggcgctgg ccgcgctcgg cacgtatggc
     1021 ctgatcgcct atgcggcgaa gcagagcacg cacgagatcg gcatccgcat ggcagtgggt
     1081 gcgagtcgtg ggcacgtcgc gcggaggttc ctgcaacgcg gacttcgtct cggcgccatc
     1141 ggcgcgctgc tcgggctcct cgcggcgggc gcgctgacgc gcctgctggc cgggctggtg
     1201 tacggcgtga gcgtgttcga cgccgtgtcg ttcgtctcgg cgctcgccgc agtgctcctg
     1261 gcggtcctca ccgcctcgtc gatcccggcg tggcgcgcct cgaggaccga tccgatcgcc
     1321 gcgctgcggc atcggtagcg tcgaagccag tcgcaccgac gcgcagggcc cctccgtcgc
     1381 cttggcgacg gcggggcagg ttcgagcgac tgaaggtacg gtggcagagc gtcagtagcg
     1441 gcgacgatcg cgatcgctgt agcgaatacc gttgcgagtg ccggcgtgat ctccatagcg
     1501 atcacggccg cgctccacga ctgccgatcg ctgcatctgc gccaggacgt caatgacggc
     1561 tgcccagtca tcccaggtgt gctcgaagac atgggcattg cgaagtcgac gatcgacctg
     1621 tcgcgcatca tccgtcaggt ggtcgatctc gcctggaaca ttccaggggg cttggcgata
     1681 ccggtccatg cggcgatgaa agtccgccgc ccgccgtgcg aaatgcctga tgtcgttcag
     1741 aaaacgctgc tctgagcgtc ctccgtggtg cgcgccgtcg atcgcctgac gtacggcgtg
     1801 ttgcgctcgc tcgtcgagaa cgtgagcgag cccgcgcatt ctctcgtagg tccgttgctc
     1861 gaattggtac tgcggccgat cgtcgccgtg tgcaccggta atggccggaa tggcaagtcc
     1921 aacgccgagc atcggcacga tgagggacac ctttggaaag cgcatgatgg gcctcctgcg
     1981 tattcgagtc aggcctcgtt cactcgactc agggggcaag gtccatgccg ttccagggtc
     2041 cggttttatt gaagcattgc cgcggcgttg tcccgtccgt gcgtcgtcct gtcatctgga
     2101 cgctccatca cagccgctcg agacctcgac tggcccggca cgcgatagga tcggaagcca
     2161 tgaatgcatg tgtcttgcgg actgcttacg tcgggctctg cctgcttgcc tgcctctcga
     2221 tggagacacg cgcgatcgcc cagaacgccg gcgcccccgc cgcgcagccc gcgccgccgc
     2281 tcaccttcgc cttcgagctg cgtgcggcgg ttggcgatcc cgtcgagatc ggccagatcc
     2341 ctcacggccg ccggcggatc gttccaatcc tgggcgggac ggtgcgcggt ccgatgttga
     2401 ccgcggccgt ggtgccgggc agcgggtccg attggcagct cattcaaccg gacgggttct
     2461 cggagctcga tacgcgctac acgctccgca ccgagaaggg ccagctcgtg tacgtgcaga
     2521 acgccggcat ccgccacgcc gcgccggagg tcatgaagaa gctgcttgcc ggagaggtcg
     2581 tcgacccgaa gctcgtctac ttccgcaccg tgccgaaatt cgagacgtcg gcgccggacc
     2641 tgcaatggct gacgcgcagc gtcttcgtgg gtgtgggtga acggtttccg agcgaggtgg
     2701 tcgtgcggtt ctatcggctc gagtagggga tcaggaaggc gctcaggccg tccgccgtcg
     2761 cgccggtggc gccccggcag gacgacctga gtaccaacct ctattcgtcg ggcgaccgga
     2821 aacgaaccgt gtgcccgtcc gcgtaggtga tcgctgacag cagcccgacg gggctcttgc
     2881 cgtcgatcgc cggcacgatc gtgactgtga tttcatcccc gggcttgagg gcgttgggcc
     2941 cttctttcag gccgagcctc gcaatctgcc ccacgctgcc gccgaggaac gtccactccg
     3001 tgaccgagcc gtccttgttc ttgacgtcga tcttcatcgt cgagtgcggg ttcacgagcc
     3061 ggaacgcctt gaacgccccg gtcacttttg cctctttgcc cagcgcgtac acgcccgcga
     3121 gggagtgatg cgcatagacc gaggcactcg ccatgatgaa ggcgaacagg acgacgaatc
     3181 gcgaaatcga tttagtcatg ttgtgcatct ccaccggact ctataagtct attagaagtc
     3241 taatgccgaa tctgcgtggc ggccgcctcg gtctcgaaac tcgtctcgca gttgccccgc
     3301 tcgcgtatga gccccccgtt ggggttcgtg ttgcgccgca gcgtccgtgt cggcaacacc
     3361 cacggctcca ccagcacctc ggggtcctcg accgtgacgt cgtacaggat tgcatcgccc
     3421 cgacgcgtga atcgctccac gacatgcatc cgatcggagt gaaagaaccc gccgcgcccc
     3481 aaccatgtcg tatcgatgaa cgcgatggcc tcgagcacca gcgtgtcgcc ttcccagcgt
     3541 cccaccgtgt tccccatgta cgttatatcg aactccgcct ctttcgtatg ct
//
LOCUS       DBNGSH010001009         3462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4731333, whole genome shotgun sequence.
ACCESSION   DBNGSH010001009 DBNGSH010000000
VERSION     DBNGSH010001009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4731333"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="AC16CY_22980"
     CDS             complement(<1..1058)
                     /locus_tag="AC16CY_22980"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR3356736.1"
                     /translation="MRPVEVLYTPESIASLDYERDLGEPGQFPYTRGIHPDGYRGKLW
                     TMRQFAGYGTPEETNQRYRRLLEAGGTGLSVAFDLPTLMGRDPDHPLALGEVGKCGVS
                     VAALEDMEALFDGIDLGAITTSMTINSPAPMLLAMYLVVAERRGTDWRALSGTIQNDI
                     LKEFIAQKEFIYSPEPSMRLVTDVIAFCTSHVPRWNTISVSGYHIREAGATAAQELAF
                     TLRNGVEYVEHAIRAGLDVDSFAPRISFFFNSHSDFFEEIAKFRAARRIWARVMRERF
                     KATSPRSWQLRFHAQTAGVSLTAQQPYNNVVRTALQAMAAVLGGTQSLHTNALDEALA
                     LPTEAAATLALRTQQIIA"
     gene            1102..2349
                     /locus_tag="AC16CY_22985"
     CDS             1102..2349
                     /locus_tag="AC16CY_22985"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011944141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3356737.1"
                     /translation="METGPIFGAMLKEFDRAARILKLDPGIWRILTYPKRQIVVSCPV
                     LMDNGEIEVFRGYRVQYNITLGPAKGGIRYHPDVTLDEVTALAAWMTWKCAVAHIPFG
                     GGKGGIVCDPTRMSQRELEALTRRYVAEIIDAIGPEKDVPAPDVNTNDQIMAWVMDTY
                     SMHVGHTETAVVTGKPIEMGGSLGRREATGRGVMIVTREAARHLGLNIKGATVAVQGF
                     GNVGSVSAELLAGLGAKIVAVTDWKGGVLNPGGLDIARLLEHVRAHKTVDGFPGGDPL
                     DNDGLWGLDVDVLIPAALENQITMDNAPQIKAKILTEGANGPTTPEAHNHLHERGVFV
                     IPDILANSGGVTTSYFEWVQDRHGYFWELDEVNVRLEKKMVEAFDDVLQTSLKYGVDL
                     RTAAYIVAISRVGMVTKMRGMYA"
     gene            2375..2764
                     /locus_tag="AC16CY_22990"
                     /pseudo
     CDS             2375..2764
                     /locus_tag="AC16CY_22990"
                     /inference="COORDINATES: protein motif:HMM:NF016056.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
     gene            complement(2800..3042)
                     /locus_tag="AC16CY_22995"
     CDS             complement(2800..3042)
                     /locus_tag="AC16CY_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356738.1"
                     /translation="MGSVGVAAWIAGIAFPTLLLIGWLTGELGPKAAAAFAILGAAAW
                     IGLPRIPNGAMLVTPALAVMDIVLVLVVFKRDIRIG"
     gene            complement(3149..>3462)
                     /locus_tag="AC16CY_23000"
     CDS             complement(3149..>3462)
                     /locus_tag="AC16CY_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356739.1"
                     /translation="RLIVADANAVPAAAREFSRTERVLVRFNAYGPGTEKPQATAVLL
                     NRGGQKMSDLAVSVTDADQGMHEITLGLASVPAGEYLVEITVKGASGEVKELVPFRVT
                     A"
BASE COUNT          610 a         1122 c         1157 g          573 t
ORIGIN      
        1 tgcgcgatga tctgctgggt ccgcagcgcc agcgtggcgg ccgcttctgt cggcagcgcg
       61 agcgcttcgt ccagcgcgtt ggtgtgcagc gactgcgtgc cgcccagcac cgccgccatc
      121 gcctgcagcg ccgtgcggac gacgttgttg tagggctgct gggcggtcag cgacacaccc
      181 gccgtctggg cgtggaagcg gagctgccac gaccgggggc tcgttgcctt gaaccgctcg
      241 cgcatgaccc gggcccagat gcggcgtgcc gcgcggaact tggcgatctc ttcgaagaag
      301 tcgctgtggg agttgaagaa gaaagaaatc ctcggggcga agctgtcaac gtcgaggccc
      361 gcccggatgg catgctccac gtactccacg ccgttgcgca gcgtgaaggc gagctcctgc
      421 gccgcggtcg ctcctgcctc gcggatgtga tatccgctga ccgaaatggt gttccaccgg
      481 ggcacgtggc tcgtgcagaa ggcgatgacg tcggtgacga gccgcatcga cggctccggg
      541 gaatagatga actctttctg ggcgatgaac tccttcagga tgtcgttctg gatcgttccg
      601 gagagcgccc gccagtcggt tccccggcgc tccgcgacga ccagatacat cgcgagcagc
      661 atgggagccg gcgagttgat cgtcatcgac gtcgtgatcg cgccgaggtc gataccgtcg
      721 aacagcgcct ccatgtcctc gagcgccgcg acgctgacgc cgcacttgcc cacctcgccg
      781 agcgcgagcg ggtgatcggg gtcgcggccc atcagggtcg gcaggtcgaa agccacgctc
      841 aggccggtgc cgcccgcctc gagcagccgg cggtagcgct ggttggtctc ttcaggcgtg
      901 ccgtacccgg cgaactgccg catcgtccag agcttgccgc gatagccgtc cggatgaatg
      961 ccgcgcgtgt acgggaactg gccgggctcc cccaggtcgc gctcgtagtc gagagaagcg
     1021 atgctctccg gcgtgtagag gacctcgact ggtctcacag tggaattata tcggtctgcg
     1081 gagacagact aagattcctc catggaaacc ggacccatat tcggcgcgat gctgaaggag
     1141 ttcgatcgcg ccgcgcggat cctgaagctc gatccgggta tctggcgcat cctcacctac
     1201 ccgaagcggc aaatcgtcgt ctcgtgcccg gtgctgatgg acaacggcga gatcgaagtc
     1261 ttccgcggct accgtgttca gtacaacatc acgctcggtc cggcgaaggg cggcatcagg
     1321 tatcaccccg acgtgacgct cgacgaggtg acggcgctcg cggcctggat gacgtggaag
     1381 tgcgccgtcg cgcacatccc gttcggcggc ggcaaggggg gcattgtctg cgatccgacc
     1441 aggatgtcgc agcgggagct cgaggcgctg acgaggcggt acgtcgccga gatcatcgac
     1501 gccatcggtc ccgagaagga cgtcccggcg cccgacgtca acaccaacga tcagatcatg
     1561 gcgtgggtga tggataccta cagcatgcac gtcggccaca ccgaaacggc cgtcgtcacc
     1621 ggcaagccga tcgagatggg cggatcgctc ggacgccgcg aggcgactgg ccgaggggtg
     1681 atgatcgtga cgcgggaagc cgccaggcac ctcgggttga acatcaaggg cgcgacggtc
     1741 gccgtgcagg gattcggcaa cgtcgggtcg gtgtcggcgg agctgctcgc cggcctcggc
     1801 gcgaagatcg tggcggtgac cgactggaag gggggcgtgc tcaacccggg cggcctcgac
     1861 atcgccaggc tgctggagca cgtgcgggcg cacaagaccg tggacggctt ccccggcggc
     1921 gatccgctcg acaacgacgg cctctggggc ctcgacgtgg acgtgctgat tcccgcggcg
     1981 ctcgaaaacc agatcacgat ggacaacgcg ccgcagatca aggcgaagat cctgaccgag
     2041 ggagccaacg gtcccacgac tcccgaggcg cacaaccacc ttcacgaacg cggagtgttc
     2101 gtcatcccgg acatcctggc caactcgggc ggcgtcacca cgtcgtactt cgaatgggtc
     2161 caggaccgcc acggctactt ctgggagctg gacgaagtga acgtgcgcct cgagaagaag
     2221 atggtcgagg cgttcgacga cgtgctgcag acctcgttga agtacggcgt ggaccttcgc
     2281 accgccgcct acatcgtcgc gatctcgagg gtcgggatgg tcaccaagat gaggggcatg
     2341 tacgcatagc caccggcacc tcgtcctcta cgacggcgtg tgcggcctct gcaatcgcct
     2401 gaacctgttt ctcctgaccc gcgacgtgca ggaggtcttc tcgtttgcct cgctccagag
     2461 ccagaccggc cagtcgctgc ttcagcggtt cggcaaggcg accggggacc tcaccacctt
     2521 ctacgtggtg acggactacc gcgccgactc gcctgcgctg ttgtcgaaat cacgcgcggc
     2581 cttgttcgtg gtgtcgaggc ttggcggtgt ctggccggcg ctgggaacgt tcggcgtgtt
     2641 gccaacggcg atgctggacg ccgtgtacga cctggtcgcc cgccatcgct accgcttctt
     2701 cggccgctac gagacttgcc cggtaccggc cccggagcac aggcggcgat tcatcgacga
     2761 ctgaaccgca ccgtcgcacc ctcgcactgt agcatccgct cacccgatgc ggatgtctcg
     2821 cttgaacacc acgagcacca ggacgatgtc catgacggcg agggcgggcg tgacgagcat
     2881 cgcgccgttg gggatccgcg gcaggccgat ccatgccgca gcgccgagga tcgcgaaagc
     2941 agcggcggcc ttcggaccca attcgccggt cagccagccg atcaggagga gagtcggaaa
     3001 ggcgatcccg gcaatccacg ccgccactcc gaccgaaccc atggctaagt gatacggacg
     3061 ttaaccacag aggcacagag aacacagagg aaacccaagt ctctgcgtcc tctgagtctc
     3121 tgtgtttgac gtccgtgacg tcgcaccgtt aagcagtcac ccggaacgga acaagctcct
     3181 tcacttcccc gcttgcgccc ttgacggtaa tctcgacgag atactcgccg gccggaacgc
     3241 tcgccagccc cagggtgatt tcgtgcatgc cctggtcggc atcggtcacc gacacggcaa
     3301 gatcggacat cttctgcccg ccgcggttca gaaggacggc tgtggcctgc ggcttctccg
     3361 tcccgggtcc gtaggcgttg aaccggacca gcacgcgctc ggtgcgggag aactcccggg
     3421 cggccgcggg aacggcgttg gcatcggcga cgatgagccg ga
//
LOCUS       DBNGSH010001010         3414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_996106, whole genome shotgun sequence.
ACCESSION   DBNGSH010001010 DBNGSH010000000
VERSION     DBNGSH010001010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_996106"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1424)
                     /locus_tag="AC16CY_23005"
     CDS             complement(39..1424)
                     /locus_tag="AC16CY_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3356740.1"
                     /translation="MPRPPRSKICDSILDAIGNTPMVRINAITRGVTEAAVLAKVETF
                     NPGNSLKDRIALRMIEDAERSGRLKPGGTIVEGTSGNTGMGLAIVAAIKGYRCIFTTT
                     DKQSKEKIDALKAFGAEVIVCPTDVDPEDPRSYYSVSSRLASEIPGAWKANQYDNPSN
                     TAAHYEQTAPEIWDQTGGSVTHLVVGVGTGGTISGIGRFLKERNPAVKVWGIDTYGSV
                     FKKYKETGIFDKNEIYPYITEGIGEDFIPGNVAFDVVDHFEKVTDKDAAIMTRRLARE
                     EGIFVGNSAGAAMAGVVQLAEHFKKGDVVVVVFHDHGSRYMGKMFNDEWMGSRGFLEV
                     TGTTARDLVATGVSGELFAVEADRSVEEAARLMSAHDFSQVSITKHGRIVGSLNETHL
                     YEEMVKNRSVKKQPIETIMQPAFAYVDISTPVELLSTMITPENPAVLIRDFKTDKTYI
                     ITRSDVIRVLM"
     gene            complement(1560..2753)
                     /locus_tag="AC16CY_23010"
     CDS             complement(1560..2753)
                     /locus_tag="AC16CY_23010"
                     /inference="COORDINATES: protein motif:HMM:NF012377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HIR3356741.1"
                     /translation="MSARPGQHVLEAAIEDNPSSRPVVQRLVKPVLMRSLRLVLLLAA
                     CSAPAFGQSPSWVAVSETGDGFVLSNQRAAFIPWGFNYFRDERFRLLEDYWNGDGPDG
                     WPKIERDLREMKRLGANVVRVSLQFARFMDAPRSPNQISLARLEKLIGFAEDVGVYLD
                     VTGLGTFRVADEPSWYRNLTEKERWASQAAFWEAVAAVGANRPGVFAYNVMNEPLVST
                     TPRRAGEWTHPAELGGMRYLEYINLDPAGRRAPDIARAWLRQMTQAIRRHDQRHPITV
                     GMIWIDNAKPENMAGFPPADIALEVDFLAVHVYPASGQVDVALDSLARYRKGKPVVVE
                     ETFPLNCTPAEYADFLRRSRGIASGWLAHFWSLTPEDLKGRTDAASGLMLESLNVFQS
                     LNPNR"
     gene            complement(2761..3318)
                     /locus_tag="AC16CY_23015"
     CDS             complement(2761..3318)
                     /locus_tag="AC16CY_23015"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3356742.1"
                     /translation="MHPQLEAIASEFLSASERLARLRQGLPPEAWSRRPAPEKWSPGE
                     CVAHLNLTSAAFVPLLRQGLDECRRSGRGVGGRYRRDLVGWLLWRALSVPGRFKSKTT
                     AAFVPSGDRPAGELAAEFERLQAEQIALTRASDGLPIDRVKIVSPFDGRVRYSIFAAL
                     SILPRHQHRHLWQAEQALGARANLT"
BASE COUNT          552 a         1139 c         1080 g          643 t
ORIGIN      
        1 cgtcggcgca acggcgtggc gcgccgccgg agggcgggtc acatcaatac gcgaattacg
       61 tccgaccgcg tgatgatgta ggttttgtcg gtcttgaagt cgcggatcag caccgccggg
      121 ttctccggcg tgatcatcgt ggacagcagc tccacgggcg tcgagatgtc cacgtaggca
      181 aacgccggct gcatgatcgt ttcgatcggc tgcttcttca cgctccggtt cttcaccatc
      241 tcttcataga ggtgcgtctc gttcagcgat ccgacgatgc ggccgtgctt ggtgatcgag
      301 acctgcgaga aatcgtgggc gctcatcagc cgcgcggcct cctcgacgga ccggtcggcc
      361 tcgacggcga acagctcgcc tgagacgccc gtcgccacca ggtcccgcgc cgtcgtcccg
      421 gtgacctcca ggaatcccct ggaccccatc cactcgtcgt tgaacatctt ccccatgtac
      481 cgcgagccgt ggtcgtgaaa cacgaccacg accacgtcgc ccttcttgaa gtgctcggcg
      541 agctgcacca cgcccgccat cgccgccccc gctgaattgc cgacgaaaat gccctcctcg
      601 cgcgccagcc ggcgcgtcat gatggctgcg tccttgtcgg tgaccttctc gaagtggtcg
      661 acgacgtcga aggccacgtt gcccgggatg aagtcctcgc cgatgccttc cgtgatgtag
      721 gggtagatct cgttcttgtc gaagatgcct gtctccttgt atttcttgaa caccgacccg
      781 taggtgtcga tcccccacac cttgaccgcc ggattgcgct ccttgagaaa ccggccgatg
      841 ccgctgatcg tccccccggt ccccacgccc acgaccaggt gcgtgaccga gccgccggtc
      901 tgatcccaga tctccggggc cgtctgctcg tagtgcgccg ccgtgttcga cgggttgtcg
      961 tactggttgg ctttccaggc tccgggaatc tcgctcgcga ggcgcgagga caccgagtag
     1021 taggacctgg gatcctcggg gtccacgtcg gtcgggcaga cgatgacctc ggcgccgaag
     1081 gctttcaagg cgtcgatctt ctccttcgat tgcttgtcgg tggtcgtgaa gatgcagcga
     1141 taccccttga tcgccgccac gatggccagc cccatgccgg tgttgccgga cgtgccctcg
     1201 acgatcgtgc cacccggctt gagcctgccc gatcgctcgg cgtcctcgat catccgcagc
     1261 gcgatccggt ccttgagcga attgccggga ttgaacgtct cgaccttcgc gaggacggcc
     1321 gcctcggtca cgcctcgcgt gatggcgttg atgcgcacca tgggcgtgtt gccgatggcg
     1381 tcgagaatcg agtcacagat cttcgagcgg ggaggcctgg gcaccgtgtg attctaatcc
     1441 gaagactcac tcggattgag aaggcgaacc cttgttcaca cttctcactt ctctcttctc
     1501 ggttcgtgtt cgatttcagg gttctgttcc gggttcgatt gtgtcgccgt cggtcttgat
     1561 cagcgattgg ggttgaggct ctggaatacg ttcaacgact ccagcatcag gccgctcgcc
     1621 gcgtcggttc ggcccttcaa atcctcgggc gtcagactcc agaagtgtgc gagccagccg
     1681 ctggctatgc cgcgggagcg acgcaggaaa tcggcgtact cggcgggcgt gcagttgagc
     1741 ggaaacgtct cttcgacgac aaccggcttt ccttttctat agcgggccag cgaatcaagt
     1801 gcgacgtcca cctgtccgct cgccgggtac acgtggacag cgaggaaatc cacctcgaga
     1861 gcgatgtccg cgggcgggaa cccggccatg ttctcgggct tcgcattgtc gatccagatc
     1921 atgccgacag taatcgggtg gcgctgatcg tgcctgcgga tcgcctgcgt catctgccgc
     1981 agccacgcgc gagcaatgtc cggagccctg cgccccgctg gatcgaggtt gatgtactcg
     2041 agatagcgca tgcctcccag ttccgcggga tgcgtccact cgcccgcccg tcttggcgtg
     2101 gtggaaacaa gcggctcgtt catcacgttg taggcgaaga cccccggacg attcgcgccg
     2161 accgccgcga cggcttccca gaacgccgcc tgtgaagccc agcgttcctt ctcggtgaga
     2221 ttccggtacc acgatggctc atcggcgaca cgaaacgtgc ccaggccggt cacgtccagg
     2281 tacaccccga cgtcttcggc gaagccaatc agcttttcga ggcgcgccag gctgatctga
     2341 ttcggcgagc gcggcgcgtc catgaatctg gcgaactgca ggctgacccg gaccacgttg
     2401 gcgccgaggc gcttcatctc gcgcaaatcg cgttcgattt tcggccaacc gtcgggcccg
     2461 tcgccgttcc agtagtcctc gagcaggcgg aaccgctcgt cgcggaagta gttgaagccc
     2521 cacgggatga acgccgcccg ctggttcgag aggacgaacc cgtctccggt ttcgctcacc
     2581 gccacccagg aaggcgactg gccgaaggcc ggggcgctgc aagccgcgag aagaagcacc
     2641 agccgaagcg agcgcatcag cactggtttc acgagccgtt ggacgacggg ccggctggaa
     2701 gggttgtcct cgatcgcggc ttcgaggacg tgctgtcccg gacgcgcgct catgtgctcg
     2761 tcatgtcaga ttcgcgcgcg caccaagagc ctgttcggcc tgccacagat gcctgtgctg
     2821 atggcgcggg aggatcgaca gcgcggcaaa gatcgaatag cggacccggc cgtcgaacgg
     2881 cgacacgatc ttcactcgat cgatcggcag gccgtcggag gcgcgggtca gcgcgatctg
     2941 ctcggcttga aggcgctcga actcggccgc cagctcccct gccggccggt cgccggatgg
     3001 cacgaatgcc gcagtggtct tggacttgaa gcgcccgggg acgctcagtg cccgccagag
     3061 gagccacccg accaggtcgc gccggtaccg gccgccgacc ccccgcccgg atcgcctgca
     3121 ttcgtcgagc ccctgccgca gcaagggcac gaacgccgcc gacgtcaggt tcaggtgggc
     3181 aacgcactcc ccgggggacc atttctccgg agcgggccgc cggctccacg cttcgggtgg
     3241 gagcccttgc cgcagacgcg cgagccgttc ggacgcagac aggaactcgc tggcgatagc
     3301 ttcgagctgg ggatgcacgt caggcgattc tagcggtacg aggtgcgagg gtacgagggt
     3361 gcgagggtgc gagtgcttcc ccctgcccct gccccctgcc cctgccccct gccc
//
LOCUS       DBNGSH010001011         6647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_316283, whole genome shotgun sequence.
ACCESSION   DBNGSH010001011 DBNGSH010000000
VERSION     DBNGSH010001011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_316283"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="AC16CY_23020"
     CDS             complement(<1..526)
                     /locus_tag="AC16CY_23020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356743.1"
                     /translation="MTWKLYAVGSAGAFLATYLVSNVAPVKPVQNTPVASPRAVDGAG
                     ESVDLTEQAERLRAGIAATTPYREPRRDAFRFGDSRRRTAAPPLEASEIEAPVVAPPP
                     RPPFALAGVATDAPGGTAGRTAILSSLRGVLLVREGDLVEGMFRIVSIDADAITVERV
                     DDGTSTTLRLSGSDS"
     gene            614..1354
                     /gene="kdsB"
                     /locus_tag="AC16CY_23025"
     CDS             614..1354
                     /gene="kdsB"
                     /locus_tag="AC16CY_23025"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR3356744.1"
                     /translation="MRTSALQAVAVIPARYASSRLPGKPLAEIAGRPMIEHVYRRAAE
                     ARGVDAVVVATDDPAIAAAVERFGGVARLTRPDHRTGTDRVAEVAAELRCDIVVNVQG
                     DLPLVEPGMIGEVIEPLLADASIRMSTLRKAIDDPADLGNPNIVKVVVDRNGDALYFS
                     RSAIPYGREPIETAFRHIGMYGYRRDFLLTFAALPQTPLERAESLEQLRALEHGFRIR
                     TVPTRHESIEVDTPEDLERVRQLLTTRV"
     gene            1361..3019
                     /locus_tag="AC16CY_23030"
     CDS             1361..3019
                     /locus_tag="AC16CY_23030"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR3356745.1"
                     /translation="MSQRADGRPVKYVFVTGGVVSSLGKGLAAASIGRLLEDHGYKVA
                     LQKFDPYINVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTSMVATRNSNWTTGKI
                     YLSVIQKERRGDYLGRTVQVIPHITNEIKDAIRLASREVDVVLVEIGGTVGDIESMPF
                     LEAIRQFRLDVGRDNTLYIHLTLVPYIGTAGELKTKPTQHSVRDLRSIGIQPDVLLCR
                     TDRFLDADIKRKIALFCDVPEEAVITAKDVDTIYEVPLVLKAEGLDQIILKQLGLPLT
                     QAQTGAWDELVDRIKHPVDDITIHVVGKYTGYEDSYKSLNEAVYHGGFAHRLRARLNW
                     VESEALERENGASLLEGAHGILVPGGFGSRGTRGMMKAAEYARERSVPFFGICYGFQW
                     ATVEYARHVCGLDGADSTEVDENAPHKVIYKLRDLLGVDDLGGTMRLGRYACELAPGS
                     LAERVYGTSLVHERHRHRFEFNCLYEQPLLEQGLRISGRSPDGKFAEIAELPAHPWFL
                     AVQFHPEFQSKPLKPHPLFAAFVEAAHRHKSGRLTPARVGAARG"
     gene            3029..3886
                     /gene="kdsA"
                     /locus_tag="AC16CY_23035"
     CDS             3029..3886
                     /gene="kdsA"
                     /locus_tag="AC16CY_23035"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009657274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR3356746.1"
                     /translation="MNPVPVGAFTVGGGSPLAVIAGPCVIESAAHALDLALEIRAISE
                     RCRVPMIFKASYDKANRTSRDSFRGPGLKEGLRILAEIKARTGLAVLTDIHEPSHAAP
                     AAEVVDILQIPAFLSRQTDLLVAAARTQKAVNVKKGQFLAPRDVRHAVEKITGEGNHA
                     VLVTERGVCFGYNNLVVDMRAFPILRELGYPVVFDVTHSLQLPGGGDGVSSGTIEYVE
                     PLASAGVAAGVDAVFMEVHEEPSRARSDAANALRLDRLEPLVRRLTRIDAAAREQSVA
                     LGEGSGVRS"
     gene            3883..4854
                     /locus_tag="AC16CY_23040"
     CDS             3883..4854
                     /locus_tag="AC16CY_23040"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR3356747.1"
                     /translation="MSKPPLDLSLARKVLQTEAAAILALVERLGEDFDRAVRLLLDCR
                     GRVIVTGMGKSGIICRKIAATLSSTGTPAIFLHPAEAIHGDLGVVQSDDVVIALSYSG
                     ETDEVLRLLETIKRLGARLVAITGDCRSTLAQASDVALDCRVSEEACPMNLVPTASTT
                     AALALGDALAMTLSAAKGFSQEHFASLHPGGKLGKRLMRVEQLMHRGDQVPIVRADTP
                     IPNVIYEMSSKGLGMTCVVDGNAALAGIITDGDLRRHMADGTNLLDRKASEIMTRRPV
                     TIRREILAAEALLVLEQRKITSIVVVDEESRVQGVVHVHDLWRTEMF"
     gene            4867..6093
                     /locus_tag="AC16CY_23045"
     CDS             4867..6093
                     /locus_tag="AC16CY_23045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356748.1"
                     /translation="MSDYALLAVLFALLVGLVVGKAWERYKLQDGRWIDRRRLRQTPH
                     YMLGLNYLVDNQIDQAVEELTRAAGTDSDALEIQIILGNLYRQKGQVGRAINVHQGLL
                     QRQDLRAIEHAHVLLCLGLDYRHGGFVDRAFETFQEVVRLDPQNRYAYVNLQKLYEDQ
                     RQWTEASAARQRIATIDGGRQDENRQILGFLQNEIGNTQLRDGNNQAAARTFQDAIDI
                     DARTAPAYLNLGDARERNDQPAAAVEAWEKLVQALPDRAHLAFDRLERTYRKAGSPRR
                     FVELCERLIEQNAQDWRTRLALSRHLAAAGQHRRALDLLLAALPHNPHGLTLHQEVWR
                     ALLALNLEAALVQRYVDLTRSAVFYLDPHVCLHCRYRSTELLWQCPQCHEWNTFVEER
                     IAPAKDAAAAAEMAEP"
     gene            6109..>6647
                     /gene="coaE"
                     /locus_tag="AC16CY_23050"
     CDS             6109..>6647
                     /gene="coaE"
                     /locus_tag="AC16CY_23050"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.7"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR3356749.1"
                     /translation="MKRIALTGGIATGKSHVRAEFERLGVPTIDADVLAREVVTPGTP
                     ALAQIVSRFGARVLDGGGALNRRTLASIVFPDPDARRDLERIVHPAVQKATSDWFAAL
                     DPARHPIALADIPLLYEVGREVDFDAVVATVCAPDTQIRRLMTRDALDEREAAARVAA
                     QLPAEEKARRADYVIRTDGT"
BASE COUNT         1180 a         2201 c         2209 g         1057 t
ORIGIN      
        1 gcgagtccga gccggacagc cgcaaggtcg tggacgtgcc atcgtccact ctttccacgg
       61 tgatcgcgtc ggcgtcgatg ctcacgatcc tgaacatgcc ctcgacgaga tcgccctcgc
      121 ggacgaggag cacgccgcgc agagagctga ggatcgcggt gcggccggcc gttccgccgg
      181 gcgcgtcggt agccacaccg gccagcgcaa acggcggccg aggcggaggc gcgacgacgg
      241 gcgcctcgat ttccgaggct tcgagaggcg gcgcggccgt tcgacgacgc gagtcgccga
      301 accggaacgc gtcgcgacgc ggctcgcggt acggagtcgt ggcggcaatg cccgcgcgaa
      361 gccgttcggc ctgctcggtg agatcgaccg actcgcccgc gccatccacc gcgcgcggcg
      421 aggccaccgg cgtattctgg accggcttga cgggcgcgac attcgacacg agatacgtgg
      481 ccaggaacgc tcccgccgag ccgactgcgt agagtttcca agtcatccgg ggatattcat
      541 cttaacattt cggctcatcg agccctcggt gttatgattc gtcgtgcgcc cggttacgtt
      601 tctcaatcct ttcctgagaa cgtcagcgct acaggctgtc gcagtcattc ccgcccgata
      661 cgcgtcttcc cgtcttcccg gtaaaccgct cgctgaaatc gccggccgcc cgatgatcga
      721 gcacgtgtat cgacgcgcgg ccgaggcgcg cggcgtggac gcggtggtgg tcgcgaccga
      781 cgatccggca atcgccgcgg ccgtcgaacg gttcgggggc gtggcccggc tgacccgtcc
      841 ggaccacagg acgggcaccg accgcgtggc ggaggtcgcc gccgagctgc gctgcgacat
      901 cgtcgtcaac gtgcagggcg atctgccgct cgtcgaaccg ggaatgatcg gggaagtgat
      961 cgaaccgctg ctggctgacg cctcgatccg tatgtctacg ctgcgcaagg cgatcgacga
     1021 cccggccgac ctcggcaacc ccaacatcgt gaaggtcgtc gtggaccgca acggcgacgc
     1081 gctgtacttt tcccgatcgg cgatcccgta tggccgggag ccgatcgaaa cagccttcag
     1141 acacatcgga atgtacggct accgccgcga ctttctcctc actttcgcgg cgctgccgca
     1201 aacgccgctc gagcgcgccg aatcgctcga gcagctgcgg gcactcgaac atgggtttcg
     1261 gattcgcacc gtaccaacgc ggcacgaatc catcgaagtg gatactccgg aggatctcga
     1321 acgcgttcgt cagctcctga cgacgcgtgt gtgaggtggc atgagccagc gagcagacgg
     1381 cagacctgtg aagtacgtgt tcgtgaccgg tggcgtggtg tcctccctgg gcaaaggcct
     1441 ggcggcggct tcaatcgggc ggcttctcga ggaccacggc tacaaggtgg cgctccagaa
     1501 gttcgacccg tacatcaacg tggaccccgg cacgatgagc ccttaccagc atggcgaggt
     1561 ctacgtcacc gacgacgggg cggaaacgga cctcgacctg ggccactacg agcgcttcac
     1621 cagcatggtc gccacgcgga acagcaactg gacgacgggg aagatctatc tctcggtgat
     1681 ccagaaggag cggcgaggcg actacctggg gcgcacggtc caggtgatcc cgcacatcac
     1741 taacgagatc aaggacgcca tccggctggc gagccgcgag gtggacgtcg tcctcgtgga
     1801 aatcggcggg accgtcggcg acatcgagag catgccgttt ctcgaagcca tccggcagtt
     1861 ccggctggat gtcggccgcg acaacacgct ctatatccac ctgacgctcg tgccctacat
     1921 cggcacggcc ggcgaactca agacgaagcc gacacagcac agcgtccgcg acctcaggtc
     1981 gatcggcatc cagccggacg tgctgctctg ccgcaccgat cgattcctcg acgccgatat
     2041 caagcgcaag atcgcgctgt tctgtgatgt gcccgaagaa gccgtcatca cggcgaagga
     2101 cgtagacacc atctacgaag tcccgctcgt actgaaggcg gaaggactgg accagatcat
     2161 cctgaagcag ctcgggctgc cgcttacgca agcccagacc ggcgcctggg acgagctcgt
     2221 ggaccggatc aagcacccgg tggacgacat caccattcac gtggtcggaa aatacactgg
     2281 ctacgaggac tcgtacaaaa gcctgaacga agcggtctac cacggcgggt tcgcccaccg
     2341 cctgagagcc cgcctgaact gggtggaatc cgaggcgctc gagcgggaga acggagcgtc
     2401 gcttctggag ggcgcgcacg gcatcctcgt ccccggcggc ttcgggtcac gcggaacgcg
     2461 cgggatgatg aaggcggccg aatacgcccg cgagcgctcg gtgccgttct tcggcatctg
     2521 ctacggcttc caatgggcga cggtggagta cgcgaggcac gtgtgcgggc tcgatggcgc
     2581 cgattcgaca gaggtcgacg agaacgcgcc gcacaaggtg atctacaagc tcagggacct
     2641 tctgggggtg gatgatctcg gcggcacgat gcgcctgggt cgttatgcgt gcgagctggc
     2701 cccaggctcg ctggcggagc gggtgtacgg tacgtcgctc gtccacgagc gccaccgcca
     2761 ccggttcgag ttcaactgcc tctacgagca acccttgctc gaacagggcc tgcgcatctc
     2821 ggggcgttcg ccggacggaa agttcgccga aatcgccgaa ctgcccgcgc atccctggtt
     2881 cctcgccgtg cagttccatc cggagttcca gtccaagccg ctgaagccgc atccgctctt
     2941 tgccgcgttc gtggaagcgg cgcatcggca caagagcggc aggctcacgc cggcaagggt
     3001 gggagcggcg cggggataga atgggttcgt gaacccggtc cctgtcgggg cattcaccgt
     3061 cggtggcggg agcccgcttg ccgtcatcgc cggtccgtgc gtcatcgaga gcgcggcgca
     3121 tgcgttggac ctggcgttgg agattcgcgc gatctcggag cgctgccgcg tcccgatgat
     3181 tttcaaggcg tcgtacgaca aggctaatcg aacgtcgcgg gattcgtttc gcgggccagg
     3241 cctgaaggag gggctccgaa tcctggcgga gatcaaggcg aggaccggct tggccgtcct
     3301 gaccgacatt cacgagccgt cccacgccgc gcctgccgct gaagtcgtgg acatcctgca
     3361 gattccggcc ttcctctcgc gccagaccga tctgctggtg gccgccgccc gcacgcagaa
     3421 ggccgtcaac gtgaagaagg ggcagttcct cgctccccga gacgtccggc acgccgtcga
     3481 gaagatcacg ggcgagggaa accatgccgt gctcgtgacc gagcggggcg tctgcttcgg
     3541 ctacaacaat ctggtcgtcg acatgcgggc cttccccatc ctcagagagc tcggctatcc
     3601 cgtggtcttc gacgtcacgc acagccttca actgcccggc ggcggcgacg gcgtgtcgtc
     3661 cggcacgatc gagtacgtcg aaccgctggc atcggcgggc gtcgctgcgg gagtcgacgc
     3721 ggtgttcatg gaggttcacg aagagccttc gcgggccaga agcgacgcgg caaatgcgct
     3781 tcgcctcgac cggctcgaac cgctcgtccg ccggctcaca cggatcgacg cggcagcccg
     3841 cgagcaatcg gtcgccctgg gcgaagggag cggagtccgg tcttgagcaa gcccccgctc
     3901 gacctcagcc ttgcccgaaa ggtcctccag acggaggctg cggcaatcct ggcgctcgtg
     3961 gagcgcctcg gggaagactt cgaccgtgcc gtccggctgc tgctcgactg ccgcggccgg
     4021 gtcatcgtca ccggcatggg caagtcgggc atcatctgcc gcaagatcgc cgccacgctg
     4081 tcgagcacgg gaacgccggc gattttcctg caccctgccg aggcgatcca cggagacctc
     4141 ggcgtcgtac agtccgacga cgtggtcatt gcgctttcgt acagcggcga gaccgacgag
     4201 gtccttcggc tgctcgaaac gatcaagcgg cttggcgctc ggctggtggc cattaccggc
     4261 gactgccgct ccacgctcgc ccaggcctcc gacgtggcgc tcgactgccg tgtatcggaa
     4321 gaagcgtgcc ccatgaacct ggtgccgacg gccagcacga cggccgcgct cgcgcttggc
     4381 gacgccctgg ccatgacgct gtcggccgcc aagggattct cccaggagca cttcgcaagc
     4441 cttcacccgg ggggaaagct cggcaaacgc ctgatgcgcg tcgaacaact catgcaccgg
     4501 ggcgaccagg ttcccatcgt gcgtgcggac acgccgatcc cgaatgtcat ctacgaaatg
     4561 tccagcaagg ggctggggat gacgtgcgtc gtggacggga atgcggcgtt ggccggcatc
     4621 atcaccgacg gggatctccg ccggcacatg gcggatggga ccaacctgct cgaccgcaag
     4681 gccagcgaga tcatgacgcg ccgtccggtc acgattcgac gggagatact ggcggcggag
     4741 gcgctgctgg tcctcgagca gcgcaagatc acgtcgatcg tcgtcgtgga cgaggagtcc
     4801 cgcgtccagg gcgtggtgca cgtgcacgac ctgtggcgga ccgagatgtt ctgataaccg
     4861 atcgtcatga gtgattacgc cttgctcgcc gtgctgtttg cgctgctggt gggcctggtg
     4921 gtcggcaaag cctgggaacg gtacaagctg caggacgggc ggtggatcga ccggcggcgg
     4981 ctccggcaga caccccacta catgctcggc ctcaactatc tggtagacaa ccagatcgat
     5041 caggccgtcg aagagctcac ccgcgcggcc gggaccgaca gcgacgcgct cgagatccag
     5101 atcatcctcg gcaacctcta ccgccagaag ggacaggtcg gacgtgccat caacgtccat
     5161 caggggctgc tacagcgcca ggacctgcgg gcgatcgagc acgcccacgt cctgctctgc
     5221 ctgggcctcg actaccgtca tgggggattc gtcgaccggg cgttcgagac gttccaggag
     5281 gtcgtgcgcc tggatccgca gaaccggtat gcctacgtca acctccagaa gctgtacgag
     5341 gatcagcgcc aatggaccga ggcctccgcg gcgcgtcaac ggatcgccac gatcgacgga
     5401 ggccgtcagg acgagaaccg gcagattctg gggttcctgc aaaacgaaat cggcaacacc
     5461 cagctgcgcg acggcaacaa ccaggcggcg gcccgaacgt tccaggacgc gatcgacatc
     5521 gacgcacgaa ccgcgccggc atacctcaac ctgggcgacg cccgcgagcg aaacgaccag
     5581 cccgcggcgg cggtcgaggc ctgggagaag ctggtccagg cgctgccaga ccgcgcgcac
     5641 ctggccttcg accgcctcga acgtacatat cggaaggcgg ggtcgccacg acggtttgtc
     5701 gagctgtgcg agcgcctgat cgaacagaac gcccaggact ggcggaccag gcttgcgctg
     5761 tcgcggcatc tggcggccgc cggccagcat cgccgcgcgc tcgatctgct gctggcggca
     5821 ctcccccaca acccgcatgg gctgacgctt caccaggagg tctggcgggc gctgctggcc
     5881 ctcaatctcg aagcggccct cgttcaacgc tacgtggacc ttacgcgcag cgccgtgttc
     5941 tacctggatc ctcatgtgtg cctgcactgc cggtatcgaa gcaccgagct gctgtggcag
     6001 tgcccgcagt gccatgaatg gaatacgttc gtcgaagaac gtatcgcgcc ggccaaggat
     6061 gccgccgcgg cggcggagat ggcggagccc tgacggcgcc gacgtgccat gaagcgcatc
     6121 gcgctcaccg gcgggattgc cactggcaag agccatgttc gcgcagagtt cgagcggctg
     6181 ggcgtgccga cgatcgacgc cgacgtgctt gcgcgcgagg tcgtcacacc aggaacaccg
     6241 gctctcgcgc agattgtgtc gcgctttggc gcccgggtcc tcgacggcgg cggagcactc
     6301 aaccggcgca cgctggcgtc aatcgtcttc cccgaccctg acgcgcgccg ggacctcgag
     6361 cggatcgtcc acccggccgt tcagaaggcg acgtccgact ggtttgccgc gctcgacccg
     6421 gcccggcacc ccattgccct tgcggacatt ccgttgctgt acgaggtcgg gcgcgaggtt
     6481 gacttcgacg ccgtggtcgc cacggtctgc gccccagaca cgcagatccg gcgattgatg
     6541 acacgtgacg cgctcgacga gcgggaggcg gccgcacgcg tggccgccca gctgcctgct
     6601 gaagagaaag cccggcgtgc ggactatgtg atcaggactg acggcac
//
LOCUS       DBNGSH010001012         4198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4732251, whole genome shotgun sequence.
ACCESSION   DBNGSH010001012 DBNGSH010000000
VERSION     DBNGSH010001012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4732251"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1658)
                     /locus_tag="AC16CY_23055"
     CDS             complement(168..1658)
                     /locus_tag="AC16CY_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356750.1"
                     /translation="MAMGRVAAAIASVVLVAVLVFSQGTAVPRAQGPVTFSEHVAPIV
                     FANCTSCHRPGEAAPFALMSYRDARPLAKAIASATASRAMPPWKAGPGDYAFTNARAL
                     TDEQIAVIQRWVADGMLEGDPARVPPLPRFTEGWQLGRPDLAVTMSEPFEVPARGPDL
                     YRNFVVPLNLDRDTWVRAVDFRPSARSVVHHSLFFLDATGAARERDAADPLPGFPGGM
                     GAGRVAGAGRGGGLAALLAGRGGGGDRSDDQVGRAGGGLGGWALGGRALELPEGLAFF
                     VPKGSDLILSTHFHPSGSIQREQSTVGLYFASGPPKQAFGTIQLPPIFGVLEGLDIPA
                     GEARYVIKDSFVIPVDVRAFSVGAHAHYLGKQMKLTATFPDGTVKTLLRIDDWDFSWQ
                     ERYGFRDFVTLPKGTRLDAEISYDNSAANKRNPSRPPVRVTWGEESNDEMGSIGLQVV
                     AANRGELPQLQQALADHTRQAALSRPGLRQLLQQRGARGAGAGSTR"
     gene            1769..2671
                     /locus_tag="AC16CY_23060"
     CDS             1769..2671
                     /locus_tag="AC16CY_23060"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3356751.1"
                     /translation="MAAFDVVGIGENSVDVVYRVPGAAAPNAKLSVSSSRVSCGGQVA
                     TTLVTCAAFGLRAAYAGSFGSDDNGRRVRDALAGQHVDTRHALIRDAPNRHAVIIVDE
                     RTGDRTVVWHRDVRLALGADDVSRAIVTGGRLLHVDDLDEDASIAAARMARAAGLLVT
                     SDIDRVTGRTADLVASVSVPICSWHVLSSLTGESDPEQALRKLGARKKGWLSVTLGTD
                     GAMLLDGDRLHHVPAFEVDSVDTTGAGDIFRGAFIYALLRGQEPADVLRFAVAAAGLG
                     CTKEGAIDSVPAMEDVERLLATRV"
     gene            complement(2726..>4198)
                     /locus_tag="AC16CY_23065"
     CDS             complement(2726..>4198)
                     /locus_tag="AC16CY_23065"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3356752.1"
                     /translation="SRDHAEIEQVHGRFVVRDRGSKFGTFVNGQQVTAEHALAHGDVI
                     RLGRLGRIELRFLIDDEPLSSSVLDSSIIGGFQQLKKLIEGLRSGRALVLDDVLALVL
                     DAAIEVTGAERGFIMLANAAGTLEFKLARGENKVTLDGARFDMSRKIPEEVFATGRDA
                     AIDLLEVPDDHEGTIVHGIRYVRCVPLRVVRIVERADISIDSRPIGVLYLDGRQKATL
                     MSRAAAASLTALANEAADAIESARLWREARDNARIVEELRVAFQIQQALLPRPTRSAP
                     FFDAVAASVPCREIGGDFFDYMDLPENRFGFALGDVAGKGVPAALLAAVIQGIFVGHQ
                     ASHDPGVMMAAVNRSLLTRAVEARFATAFLGVLDPGGTLIYCNAGHSPPFLFSSAGVQ
                     RLAGGGTVLGVFDGARYESVAVQLRQGDTVIVFSDGVSEARSPLGEEFGDDRISSTLA
                     PIVGQPVQIVLDALLVAVREFAATVPQNDDITAVVVRYRG"
BASE COUNT          671 a         1405 c         1489 g          633 t
ORIGIN      
        1 gggcagcgag gccatgctca tctgaatgaa gtacccggcg tgatcgatga tctcggtcag
       61 cggggcggtg atgacgattc gccgtccgcc ctggctggct ggtgtggtca tgtcgctctc
      121 gctccgatgg cgccgccgcc gcaaacggtc attgtagctg gacgggctca gcgcgtcgag
      181 ccggctccgg ctccccgggc gccgcgctgc tgaagcagct gccggaggcc cgggcgcgaa
      241 agtgcggctt gccgcgtgtg gtcggcgagc gcctgctgga gttgcggcag ctcgccgcgg
      301 ttggcggcca cgacctggag cccgatgctc cccatctcgt cgttcgactc ttcgccccac
      361 gtgactcgca cgggcggacg gctggggttt ctcttgttcg ccgccgaatt gtcgtagctg
      421 atctcggcgt cgagccgcgt ccccttgggc agcgtcacga agtcgcgaaa gccgtaccgc
      481 tcctgccacg agaagtccca gtcgtcgatc cgcaggagcg tcttgacggt accgtcgggg
      541 aacgtcgccg tcagcttcat ctgcttgccc aggtaatgcg cgtgagcgcc cacgctgaag
      601 gcacgcacgt caaccgggat cacgaacgag tccttgatca cgtaccgggc ttcgccggcg
      661 gggatgtcga gcccttcgag gacgccgaag atgggcggga gttgaatcgt gccgaaggcc
      721 tgctttggcg gcccggacgc gaagtacaac ccgacggtgg actgctcgcg ctgaatgctg
      781 cccgagggat ggaagtgcgt ggagagaatg aggtcggacc ccttggggac gaagaacgcg
      841 aggccctccg gaagctcgag cgctcgaccg ccaagcgccc agccgccgag ccctcctcct
      901 gcgcggccga cctggtcgtc ggatcgatcg ccgccgccgc cacgcccggc gagcaacgcc
      961 gccagaccgc ccccacgtcc ggcgcccgcc acgcgcccgg cacccatccc gccggggaac
     1021 cccggcagag gatctgccgc gtcgcgctcg cgcgcggcgc ccgtggcatc gaggaagaag
     1081 agactgtgat gcacgacgct gcgcgcggac ggcctgaagt caacggccct gacccacgtg
     1141 tcgcgatcga ggttgagcgg caccacgaag ttgcgataga ggtcggggcc tcgtgccggc
     1201 acttcgaacg gttcgctcat cgtgacggcg aggtcgggtc ggccgagctg ccacccttcg
     1261 gtgaatcgcg gcagcggcgg cacgcgggcg ggatcaccct cgagcatgcc gtcggcgacc
     1321 cagcgctgga tgacggcgat ctgctcgtcg gtcagcgcgc gcgcgttggt gaacgcgtag
     1381 tcgcccgggc cggccttcca gggcggcatc gcccgcgacg cggtggcgga ggcaatcgcc
     1441 ttggccagcg ggcgcgcatc gcgatagctc atgagcgcaa acggggcggc ttcgccgggc
     1501 cggtggcagg acgtgcagtt ggcgaacacg atcggggcga cgtgctcgct gaaggtgacc
     1561 gggccttgag cccggggcac cgccgtcccc tgactgaaga ccaacaccgc gaccaggacg
     1621 acgctcgcga ttgcagctgc gacacggccc atggccatcg ccacctcctc gaccagtgta
     1681 accggggcag ggggcagggg gcagggggca gggggcaggg ggcaggggtt agggggcagt
     1741 ggatagtggt tggtagactt cgtccgcggt ggctgcgttc gatgtggtcg gcatcggcga
     1801 aaactcggtg gacgtcgtct atcgtgtccc cggcgcggcg gcgccaaacg ccaagctttc
     1861 ggtgtccagc agccgggtgt cgtgcggcgg ccaggtggcc accacgctcg tcacgtgcgc
     1921 ggccttcggg ctgcgtgccg cgtatgcggg ttcattcggc agcgacgaca acggccgtcg
     1981 ggtccgcgac gcgctcgcgg ggcagcacgt ggatacgagg cacgccctga tccgcgacgc
     2041 gcccaaccgg cacgccgtga tcatcgtgga cgaacggacg ggcgaccgga cggtcgtatg
     2101 gcatcgcgac gtacggctcg cgctcggcgc cgacgacgtg tcgcgcgcga tcgtgaccgg
     2161 cgggaggctg cttcacgtgg acgacctcga cgaggacgcc tcgatcgccg cggcacgcat
     2221 ggcgcgcgcg gcgggacttc tggtcacgag cgacatcgat cgcgtcacgg gacggacggc
     2281 ggatctcgtc gcgtcggtga gcgtcccgat ctgctcgtgg cacgtgttga gctcgctcac
     2341 cggcgagtca gaccccgaac aggcgctcag aaagctggga gcgagaaaaa aagggtggct
     2401 gtcggtgacg ctgggaaccg acggcgcgat gctgctcgac ggagatcggc tccatcacgt
     2461 ccccgccttc gaggtcgatt ccgtcgatac gacgggggcc ggcgatatct tccggggcgc
     2521 gttcatctac gcgctgcttc gagggcagga gcccgccgac gtcctgcgct tcgcggtcgc
     2581 cgccgcgggg ctcggctgca cgaaggaggg cgcgatcgac agcgtgcccg cgatggagga
     2641 cgtcgaacgc cttcttgcca cgagggtctg acgaaccgcc acgacgacac gaagaccacg
     2701 agctggccac gacgacacga gcgcctcagc cgcggtaccg caccacgaca gccgtgatgt
     2761 cgtcgttctg cgggaccgtg gctgcgaatt cccggacggc gacgagaagc gcatccagga
     2821 ctatctgaac gggctgcccg acgatcggcg cgagcgtcga cgagatccgg tcgtcgccga
     2881 actcttcgcc cagcggactt cgcgcttccg acacgccgtc gctgaagacg atcaccgtgt
     2941 cgccctgccg gagctgcacg gcgaccgact cgtatcgtgc gccgtcgaag acgccgagga
     3001 cggtgccgcc gcccgcgagc cgctgcaccc ccgcggacga gaagagaaac ggcgggctgt
     3061 gtccagcatt gcagtagatc agcgtgccgc cggggtcgag gacgcccaga aacgcggtgg
     3121 cgaacctggc ttcgaccgcg cgcgtcaaaa ggctccggtt gacagccgcc atcatcacgc
     3181 ccgggtcgtg cgaggcctgg tgaccgacga agatgccctg gatgacggcg gccagcagcg
     3241 ccgcgggtac gcccttaccg gctacgtcgc cgagcgcgaa cccgaaccgg ttctccggca
     3301 ggtccatgta gtcgaagaag tcgcccccga tctcgcggca cggcaccgac gcggcgaccg
     3361 cgtcgaagaa cggggccgac ctggtcgggc gcggaagcag tgcctgctgg atctgaaacg
     3421 ccacgcgcag ctcctcgaca atccgggcgt tgtctctggc ttccctccag agccgcgcgc
     3481 tctcgatagc gtccgccgcc tcgttggcta acgccgtcaa cgaggccgcc gccgcgcgcg
     3541 acatcagcgt ggccttctgc cggccgtcga gatagagcac gccgatcggc cgcgaatcga
     3601 tcgatatgtc ggcgcgttcg acgatccgca cgacgcggag aggcacgcat ctcacgtatc
     3661 ggatgccgtg aacgattgtg ccctcatggt cgtcgggcac ctcgagcaga tcgatggcgg
     3721 catcgcggcc ggtcgcaaac acctcctcgg ggatcttgcg gctcatgtcg aagcgcgcgc
     3781 cgtcgagcgt gaccttgttc tctcctcgag cgagcttgaa ttcaagcgtc ccggctgcgt
     3841 tggccagcat gatgaatccg cgttcggccc ccgtcacctc gatggccgcg tcgaggacga
     3901 gcgcgagcac gtcgtccagc accagcgccc ggccggagcg gaggccctcg atgagcttct
     3961 tcaactgctg gaagccgccg atgatcgacg agtcgaggac cgagctggac aggggttcgt
     4021 cgtcgatgag gaatcgcagc tcgatgcgtc cgaggcggcc gagtcgaatg acgtcgccgt
     4081 gcgcgagagc gtgttccgcc gtcacctgct ggccgttgac gaacgtgccg aacttggatc
     4141 cgcggtcgcg cacgacgaag cgcccgtgga cctgctcgat ctcggcgtgg tcgcggga
//
LOCUS       DBNGSH010001013         3782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1846047, whole genome shotgun sequence.
ACCESSION   DBNGSH010001013 DBNGSH010000000
VERSION     DBNGSH010001013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1846047"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1452)
                     /locus_tag="AC16CY_23070"
     CDS             complement(172..1452)
                     /locus_tag="AC16CY_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3356753.1"
                     /translation="MNQADTAWMLMSTALVLLMTPALAFFYGGLARTKNALNTMMMSF
                     VALGFVAVVWALAGYSLAFTTGGKWIGDLSNVFLNGVGLEPKGTIPHVLFMAYQATFA
                     IITAALISGAVIERMRFSAYVIFISVWVLAVYAPIAHWVWGGGWLMQWGALDFAGGTV
                     VHVNAGIAALVAAAVIGRRSDYGSASILPHNVPFVLLGAGLLWFGWFGFNSGSAVAAS
                     PQAGLAFVTTMLAPAATLVVWTIIDAVRSGKPTTVGCATAIVVGLVAVTPAAGHISPM
                     SALALGAIAAVPSYLGLMIRPKTGLDDSLDVVAAHGVGGTVGALLTGVFAQKALNGTT
                     DGALFGNPYQLVIQSAAILAAIVYSGLGTFVILKAIALFMPLRATKSEEAEGLDITAH
                     GEEAYIHMGGSMSPAPSKEKSSAGPVFGTAPVDA"
     gene            1948..>3782
                     /gene="fusA"
                     /locus_tag="AC16CY_23075"
     CDS             1948..>3782
                     /gene="fusA"
                     /locus_tag="AC16CY_23075"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3356754.1"
                     /translation="MKVYDAASIRNVAIVGHGGCGKTQLVSTMLFVAGAVNRIGRVDE
                     GTTVTDFDDEEIARKHTLSSSLAYAEWQKIKINIIDTPGFANFLTDARAALRVAEAAI
                     VVVDAVAGVEVQTEKLWAEAVALGLPRIVALNRLDRDRASLERTLESLRRDCAREIVP
                     IQLPLGEEKAFTGVVDLVRMKALTFGSDGKMTEGDIPAALANAAQRAREQLIEMVAEA
                     DEQLMELFFAEGTLTQEQLVAGLKSATMAGKMYPLVCTSGLHATGIQPLLDAIVGYAP
                     SPAERDFRAVAPDGTETAVKASDTGPYSAFVWKTIADPFAGRITMLRVVSGALKSDAT
                     VNNATRDAAERLGHLLVLQGKAQTHVPELKAGDLGAVAKLKDTHTSDVIADKGNKVRY
                     PPITFPEPVLSYAIEPKSRGDEDKISSAMHRLEEEDPSIKYARDPQTTELLLSGQGQL
                     HIEVTVAKLKRRFGVEVNLKPPRIPYRETITASTEAHGRHKKQTGGHGQFGDCKIRVE
                     PLPRGSDFQFEDDIFGGSIPRQFVPAVEKGIQDARLRGFLAGYPMVDFKATVFDGSYH
                     QVDSNELSFKLAGSLAFKDAMTRARPTILEPIMKVEVYLPNEFAG"
BASE COUNT          724 a         1285 c         1233 g          540 t
ORIGIN      
        1 cgtgccggcg cggttgccag taacaaggcg cacgtggcca tggccagtgc aagtgcgtgc
       61 ttccgtactc tcgacatcaa gcgcatcgtc cctccgaatt caagccgtca cagttgagag
      121 cggcggcaac gtgccgccgc tttatcaggg agctcagttc gcatgtcacg cctacgcgtc
      181 cacgggagcg gtcccgaaca cgggtcccgc cgaagacttc tccttggacg gcgcggggga
      241 catcgatcca cccatgtgga tgtaggcctc ttcaccgtgg gccgtgatat ccaggccctc
      301 ggcttcctcg gacttcgtcg cgcgaagcgg catgaagagc gcgatcgcct tcagaatcac
      361 gaacgtcccg aggccgctgt agacgatggc cgcgaggatc gccgcacttt gaatgaccag
      421 ctgatacgga ttgccgaaca gcgcgccgtc cgtcgttccg ttcagcgctt tctgcgcgaa
      481 cacacccgtg agcagggcgc cgaccgtgcc gccgacgccg tgagcggcca cgacatccag
      541 agagtcgtcc aggccggtct tgggccggat catgaggccg agatagcttg gcaccgccgc
      601 aatcgcgccg agcgcgagcg cgctcatcgg gctgatgtgg ccggcggcag gcgtgacggc
      661 gacgaggccg acaacgatgg ccgtggcaca gcccaccgtc gtgggcttgc cgctcctcac
      721 cgcgtcgatg atcgtccaga cgacgagggt cgccgccggc gccagcatgg tcgtcacgaa
      781 cgcgaggccg gcctgcgggc tggcggccac ggcgcttccc gagttgaacc cgaaccagcc
      841 gaaccagagc agcccggcgc cgagcaggac gaacgggacg ttgtgcggaa ggatcgacgc
      901 actgccgtag tccgaccggc gtccgatgac cgcggccgcg acgagcgcgg cgatgccggc
      961 gttgacgtgg acgacggtgc cacccgcgaa atcgagcgcg ccccactgca tcagccagcc
     1021 tccgccccag acccagtgcg cgatcggggc gtagacggcg agcacccaga cggaaatgaa
     1081 gatcacgtac gccgagaagc gcatgcgctc gatgacggcg ccggagatga gcgccgcggt
     1141 gatgatggca aacgtggcct gatacgccat gaagagcaca tggggaatgg tgcccttcgg
     1201 ctcgagcccg acgccgttca ggaacacgtt ggagagatcc ccgatccact tccctcccgt
     1261 cgtgaaggcg agcgaatagc cggcgagggc ccagaccacc gccacgaacc cgagggcgac
     1321 gaaactcatc atcatcgtgt tgagcgcgtt cttggtccgc gcgagccccc cgtagaagaa
     1381 ggcgagcgcc ggcgtcatca gcaacacgag ggcagtggac atcaacatcc aggcagtgtc
     1441 agcttgattc attcgtcacc tctgttccgc atctggaacc gatcacccga gccaagggcg
     1501 gcgcgatcca cctgagcaaa acggacgcca gcggacggac ccgcggggac gcgggccgga
     1561 tcgaccggga tcggcccgaa tccgcggatc catcgcgccg ggagagcagt tcgcggggct
     1621 caactctcac gacattcctg tcgtactaat aggtgtgtgg aacgtaaatg tcggaatcga
     1681 tacccgtcca ggcggccgcg cgtcccccgg cacacccgtc aggcgcacac cggatgcggc
     1741 gcgggtccgg ccggaacgca tggatcggag gggtggccgc gatcgtcaga caattctgtc
     1801 ctacagatgt tcgtatacga catgattgtt ggtatcgtgt gcggccatgg gcgcaggcgc
     1861 cgccgcggac gcccggccct ccgatgcctt gctacccccc aaccggcgtg actatgatcc
     1921 cctcttgcgc gaggagggat accccgtatg aaggtctacg acgccgccag catccgcaac
     1981 gtcgccatcg tcgggcacgg cggatgcggc aagacccagc tggtctcgac catgctgttc
     2041 gtcgcgggcg cggtcaaccg catcggccgg gtggacgaag gcacgaccgt caccgacttc
     2101 gacgacgaag agatcgccag gaagcacacc ctctcctcca gcctcgccta cgccgagtgg
     2161 cagaagatca agatcaacat catcgatacg ccggggttcg ccaacttcct gaccgacgcg
     2221 cgcgccgcgc tgcgagtggc cgaggcggcc atcgtcgtgg tggacgccgt cgcaggcgtc
     2281 gaagtgcaga ccgaaaagct ctgggccgag gcggtggcgc tcggcctgcc ccgaatcgtc
     2341 gcgctcaacc gtctcgatcg cgaccgagca agcctcgagc gcaccctcga gtcgctgcgc
     2401 cgcgactgcg cccgcgaaat cgtcccgatt cagctcccgc tcggcgagga gaaagcgttc
     2461 accggcgtcg tcgacctcgt gcggatgaag gcgctgacgt tcggaagcga cggcaagatg
     2521 acggaaggcg acattccggc ggcgctcgcc aacgccgccc agcgcgcgcg ggagcagctc
     2581 atcgagatgg tcgccgaagc cgacgagcaa ctgatggagc tattcttcgc cgaggggacg
     2641 ctcacccagg agcaactcgt cgccggcctc aagtccgcca cgatggccgg caagatgtac
     2701 ccgctggtgt gcacgtccgg cctccacgcc accggcatcc agccgttgct cgacgccatc
     2761 gtcggctacg cgccgtcccc cgcggagcgc gatttccgcg cggtggcgcc ggacgggacc
     2821 gagacggcgg tgaaggcgtc ggacacgggc ccgtattcgg ccttcgtctg gaagacgatc
     2881 gccgacccgt tcgccgggcg catcacgatg ctccgcgtcg tctcgggcgc cctgaagtcg
     2941 gacgcgaccg tgaacaacgc cacgagggat gcagccgagc ggctcggcca cctgctcgtc
     3001 ctccagggca aggcccagac ccacgtgccc gagctcaagg ccggagacct cggcgccgtc
     3061 gcgaagctga aagacaccca cacgagcgac gtcatcgcgg acaagggcaa caaagtgagg
     3121 tacccgccca ttacgttccc cgagccggtc ctgtcgtacg cgatcgagcc gaaaagccgt
     3181 ggcgacgagg acaagatcag cagcgccatg caccggctcg aggaagagga cccgtcgatc
     3241 aagtacgcgc gcgatccgca gaccaccgaa ctcctgctgt cgggccaggg ccagctccac
     3301 atcgaagtca ccgtcgccaa gctcaagaga cggttcggcg tcgaagtgaa cctgaaaccg
     3361 ccgagaatcc cctaccgcga gacgatcacg gcctccaccg aggcgcacgg ccggcacaag
     3421 aagcagacag gcgggcacgg ccagttcggc gactgcaaga tccgcgtgga gccgctgcca
     3481 cgcggcagcg atttccagtt cgaggacgac atcttcggcg gatccatccc ccgacagttc
     3541 gtcccggcgg tggaaaaagg catccaggac gcccggctgc gggggttcct ggccggctac
     3601 ccgatggtgg acttcaaggc gaccgtgttc gacgggtcgt atcaccaggt cgactcgaac
     3661 gaactgtcgt tcaagctggc cgggtcgctc gccttcaagg acgcgatgac gcgggcgcgg
     3721 cccaccatcc tcgagccgat catgaaggtc gaggtctacc tgcccaacga gttcgcggga
     3781 ga
//
LOCUS       DBNGSH010001014         2539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2525456, whole genome shotgun sequence.
ACCESSION   DBNGSH010001014 DBNGSH010000000
VERSION     DBNGSH010001014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2525456"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..>2539
                     /locus_tag="AC16CY_23080"
     CDS             39..>2539
                     /locus_tag="AC16CY_23080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR3356755.1"
                     /translation="MKSARTFVDRLLSLFRRSGLDNDLDDEVRTHLELLARDHERRGM
                     SASDARLAARRDFGGVEQMKEAYRDRRGLPWVDDLHRDVRYAVRALARNPGFTAVALL
                     VVALGIGANTAIFSLLDAVILRPLPVRQPDELVLGSYQVEGRRTLPFTAYQFRALRAN
                     RDVLADMAAFRPLPLSVTFRGDSELAFGQLVSGSYHAMLGVPAALGRTLMASDDSALV
                     GDRPAVISYGYWQRKFGGARTVLGESIEINAIPYTIVGVTERRFFGTEPGLAVDVTIP
                     MSTQVGVLGARSLIDEASNARWLYLIGRLAPGVSRERATSTLTVTWDRLRASRAPAGR
                     ALPPQPFALLDGAQGRNVLRDRFSQPLRVLMAMVGVVLIIACANLATLLLARSGARRQ
                     EVGLRLSLGASRSRLLRQLLTESLLLSMIGGAIGVGLAYVASDVLVQIMSRGVDAIVL
                     DLAPNGRTLAFTLLASLGAGLVFGILPSLRAARWGVATAARVAPSPAHGGRRWSQATI
                     AAQVMLSVLLLVEAGLFVRSLTALRGLDTGFTNGNQVLLASIRTRSGNTDELVRILDL
                     FNELSARPDSLSARSVTFSVDMPFVEGLSFAQNIEVPGRPPDGNEAAVWFNFVGPRFF
                     ETMGIPLEGRGFRGDDVESAPAVAVISQGLARRYFPGVNAIGKRVRTNDVEVEVVGVA
                     PDVPYTSLRAVPTEMIYLPFFQGRVAKNVGIITMAVRAAGRADQTAAALRREVPAVSS
                     DLLISSLVTLDERRDASLARERVVATLSMWFGLLALLLGCVGLYGTLSYAVTRRTSEL
                     GVRVALGADRPRLVGMVLAESLRPVLVGITLGLPLA"
BASE COUNT          354 a          824 c          895 g          466 t
ORIGIN      
        1 gccgcgagcg tggatcccgt ggtggcgttg agagctgaat gaagtctgct cgcaccttcg
       61 tcgatcggct cctgtcgctg ttccggcgtt ccggtctcga caacgacctg gacgacgagg
      121 tgcgcacgca cctcgagctg ctggcgcgag accacgaacg ccgtgggatg agcgcgagcg
      181 acgcgcgtct cgcggcgcgg cgcgacttcg gcggcgtgga gcagatgaag gaagcctatc
      241 gcgaccgccg gggattgccg tgggtcgacg acctgcaccg cgatgtccgg tacgcggtgc
      301 gggcgctcgc caggaatccc ggcttcaccg cggtggccct gctggtcgtg gcgctcggca
      361 tcggcgcgaa cacggcgatc ttcagcctgc tcgatgccgt gatcctgcgg ccacttccgg
      421 ttcggcagcc cgacgagctc gtactgggca gctaccaggt cgagggccgc cgcacgttgc
      481 cgttcaccgc gtatcagttt cgcgccctcc gcgcgaaccg cgacgtgctt gccgacatgg
      541 cggccttccg gccgttaccg ctcagcgtca cgttccgggg cgactccgag ttggcgttcg
      601 gtcagctggt ctctggaagc tatcacgcga tgctgggcgt gcctgccgcg ctcggcagaa
      661 ccctgatggc ttcagacgat tcggcgcttg tcggcgatcg gccagccgtc atcagctacg
      721 gctactggca gcggaagttc ggcggcgccc gcacggtgct cggcgagtcg atcgagatca
      781 acgcgatccc gtacacgatc gttggcgtca ccgaacgcag gttcttcggg acagaacccg
      841 gtctcgcggt ggacgtcacg attccgatgt cgacgcaggt gggggtgctc ggcgccaggt
      901 cgctcatcga cgaagcgagc aacgcacgct ggttgtacct gattggccgt ctcgcgccag
      961 gggtgtcacg agagcgcgcg acgagcacgc tgacggtgac atgggatcgg ctccgggcgt
     1021 cgcgtgcccc cgccggccgc gctttgcctc cgcagccatt cgcactgctc gacggcgctc
     1081 aaggacgcaa cgtcctgcgc gaccggttct cgcaaccact tcgcgtgctg atggcgatgg
     1141 tgggcgtggt cctgatcatt gcgtgcgcga acctggcgac gctgctcctc gccaggtccg
     1201 gcgcgcggcg tcaggaagtg ggcctgcggc tctcgttggg ggccagccgt agtcgcctgc
     1261 tgcggcagtt gctgaccgag agcctgttgt tgtccatgat cggaggcgcc atcggcgttg
     1321 ggctggccta cgtggccagc gacgtactcg tgcagatcat gtcacggggc gtcgacgcga
     1381 tcgtgctcga cctcgcaccg aacggcagga cgctcgcgtt tacgctgctc gcgagcctgg
     1441 gtgccggcct ggtgttcggc atcctgccct cgttgcgcgc ggcccgctgg ggcgtggcga
     1501 cggcggcccg cgtcgcgccg agcccggccc acggcggccg ccgctggagc caggcgacga
     1561 ttgccgcaca agtcatgctc tcggtgctgc tgctcgtgga ggcgggactc ttcgtgcgca
     1621 gcctcacggc gcttcgaggg ctggacaccg ggttcacgaa cggaaatcag gtgctgctgg
     1681 cgagcattcg cacgcgctcg ggaaacaccg atgagctcgt ccgcattctg gatctcttca
     1741 acgagttatc tgcgcgtccc gattccctga gcgcccgttc agtcaccttc tcggtagaca
     1801 tgccgttcgt cgaagggctt tcgttcgccc agaacatcga agtgcccggc cgcccaccgg
     1861 acggcaacga ggcagcagtc tggttcaact tcgtcgggcc gcgcttcttc gagacgatgg
     1921 gcattccgct cgaaggccgc ggcttccgag gcgacgacgt tgagagtgcg cccgcggtgg
     1981 cggtgatcag tcaagggctg gctcgacggt actttccggg cgtgaatgcc attggcaagc
     2041 gcgtgcggac aaacgatgtg gaggtcgagg tcgtcggcgt cgccccggat gtcccgtaca
     2101 ccagcctgcg cgcggttccc accgagatga tctatctgcc gttctttcag ggacgcgtcg
     2161 cgaagaacgt cggcatcatc acgatggccg tccgtgcggc gggacgcgcg gaccagacag
     2221 ccgcggcgct gcggcgcgag gtaccggccg tctcgtccga tctgttgatc tcgagtctgg
     2281 tgacgctcga cgaacgaagg gacgcatcgc tggcgcgcga gcgagtggtg gcgacgctgt
     2341 cgatgtggtt cggcttgctc gcgctgctgc tcggctgcgt cgggttgtac ggaacgttgt
     2401 cctatgcggt gacccggcga accagtgagc tcggagtacg cgtggcgctc ggtgcggacc
     2461 ggccgcggct ggtcggcatg gtgctcgctg aatcgctccg accggtgctg gtgggcatca
     2521 cgctcgggtt gccgctcgc
//
LOCUS       DBNGSH010001015         5156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_148542, whole genome shotgun sequence.
ACCESSION   DBNGSH010001015 DBNGSH010000000
VERSION     DBNGSH010001015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_148542"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="AC16CY_23085"
     CDS             complement(<1..302)
                     /locus_tag="AC16CY_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HIR3356756.1"
                     /translation="MAEVANRLASEQSPYLLQHKDNPVDWYPWGQEAFDRARRENKPI
                     FLSVGYSTCHWCHVMEHESFEDAAIAEVLSRYFVSIKVDREERPDVDRVYMTFVQMT"
     gene            complement(346..1248)
                     /locus_tag="AC16CY_23090"
     CDS             complement(346..1248)
                     /locus_tag="AC16CY_23090"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3356757.1"
                     /translation="MAGRRAWRRLRLELRLSGQAAWRAAVGLVKGNDLTHAASIAFYG
                     VLCLFPFLLLVMSVLGSITADEADRAQALAFLFRNFPARLDFLTDQLDALRQKRLGIG
                     VAGALGLMWASLGFFGAVTSAVDEAWGVERQRSFWKHRLVSFLMLVAAGSTLAVALVL
                     VSFFHVAQASWFGQILASFGWLVTLQTFVFRYLATLLLVLGVGLVFYFIPNARTRFRD
                     VWVGALLTGVLWRAAFSGFAWYIRQNEGLSMIHGSIAAVIVFLLWIYISSAILMYGVE
                     FTAAYARLRRHRRDEQSAAATPRT"
     gene            complement(1273..2682)
                     /locus_tag="AC16CY_23095"
     CDS             complement(1273..2682)
                     /locus_tag="AC16CY_23095"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3356758.1"
                     /translation="MHPQLRRRRRMLVVDGDSGMFPWLERVVESEPFDVRGAISGLQG
                     EEIYRQWRPHIVLTDLAPPDGDGIELLQRLKRVDPEPEVIVIGGQGTITRAVEAGRAG
                     AFFFLERPVSEEGLLDLLGKAAARIDERVEHQQLKERVRGQYSFANVIGQSKAMTELF
                     ELVESVARSDANILIQGENGTGKELIANAIHYNSHRAGGPFIKINCASIPKDLIESEL
                     FGHKKGAFTGATSDKVGLVEMAEGGSLLLDEIGEMPGYLQTKLLRVLQERAYRPVGGD
                     RLVTVDFRLICATNVDLDVALREGRLREDLYFRINTITLRVAPLRERTEDIPLLCNHF
                     LAKFRQRYQKSVRSISPAVYHLLIRNRWPGNVRELENAIERSVLVCKSGEITPSDLPE
                     SIRETVAGTHDFTIPPHRTLAEIERMAIVQTLQRTNWNKQEAAHILGLYRPTLYSKMK
                     KHEITDGRAAARRAGGGQQ"
     gene            2832..4439
                     /locus_tag="AC16CY_23100"
     CDS             2832..4439
                     /locus_tag="AC16CY_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3356759.1"
                     /translation="MQVLETHVRPEAQLFQDNARRMSTLVEELRARLATARAGGGERY
                     LQRHRELGKLPVRTRIDRLLDTGSPFLELSPLAAWDVYDNDAPGAGIVTGIGRVSSKE
                     VLIVANDATVKGGTYYPATVKKHLRAQHVALENQLPCIYLVDSGGAFLPLQAEVFPDR
                     DHFGRIFFNQARMSALGIPQIAVVMGSCTAGGAYVPAMSDETIIVQGTGTIFLGGPPL
                     VKAATGEEVTAEELGGADVHTRLSGVADYLADDDDHALRLARTIVGTLNRVKTMPADM
                     TSPEDPAYDPREIYGIVNADVRKPYDVREVIARLVDGSRFDEFKQRYAPTIVTGFARL
                     HGFLVGIVANNGVLFSESALKATHFIELCNMRSVPLIFLQNITGFMVGRQYERGGIAK
                     DGAKLVHAVANSVVPKFTVVIGGSFGAGNYGMCGRAYDPRLLWMWPNARISVMGGEQA
                     AGVLTAVKREQLGREGKSFSAEEEQAIRGPLLEKYEHEGSPYYSTARLWDDGILDPAE
                     TRQALALGISAAYNAPIPAAKYGIFRM"
     gene            4561..>5156
                     /locus_tag="AC16CY_23105"
     CDS             4561..>5156
                     /locus_tag="AC16CY_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006376631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HIR3356760.1"
                     /translation="MTESTSFSRILVANRGEIAVRIIRACREMGIESVAVYSDADARA
                     LHASLADVAVGIGPAPAAESYLSIDAILAAAATTGSQAIHPGYGFLSQSTRFASACQA
                     AGLVFIGPPPEAMARMGSKIAARRLAHDAGVPVVPGEIPGDQSDQAIADAARRIGFPV
                     LLKPSEGGGGIGMKTARDARELDALIGQARREAQAAFGA"
BASE COUNT          912 a         1732 c         1664 g          848 t
ORIGIN      
        1 gtcatctgga cgaacgtcat gtagacgcga tccacatccg gccgctcctc ccggtccacc
       61 ttgatcgaca cgaagtagcg cgagagcacc tcggcgatcg ccgcgtcctc gaagctctcg
      121 tgctccatga cgtggcacca gtggcaggtc gaatagccga ccgacaggaa gatcggcttg
      181 ttctcccgcc gggcacggtc gaatgcttcc tgaccccacg ggtaccagtc gaccgggttg
      241 tccttgtgct ggaggaggta gggactctgc tcgctcgcga gtcgattggc cacttcagcg
      301 attatgtatc gagccagccg agagccaggc gccgagagaa agccgtcacg ttcggggcgt
      361 cgccgctgct gactgctcgt cgcgccgatg ccggcgcagt ctcgcgtacg ccgcggtgaa
      421 ctccacgccg tacatgagga tggccgatga gatgtagatc cagaggagaa acacgatcac
      481 ggcggcgatc gagccgtgaa tcatgctcag cccttcgttt tggcggatgt accaggcgaa
      541 acccgagaag gcggcacgcc acaggacgcc ggtcagcagc gctcccaccc acacgtcacg
      601 gaaacgggtc cgggcgttcg ggatgaagta gaagacgaga cccactccga gcacgagcag
      661 caacgtcgcc aggtacctga acacgaacgt ctgcagcgtc accaaccagc cgaagctcgc
      721 caggatctgc ccgaaccacg atgcctgcgc cacatgaaag aagctgacga ggacgagcgc
      781 cacggccaga gtgcttccgg ccgccaccag catgagaaag gaaaccagcc ggtgcttcca
      841 gaaactacgc tgcctctcca cgccccaggc ttcgtcgacc gcgctcgtga cggcgccgaa
      901 gaagccgagc gacgcccaca tgagccccag cgcgcccgcc acgccgatac ccagccgctt
      961 ctggcggagg gcgtcgagct ggtcggtgag gaaatcgagt cgggcgggaa agtttcggaa
     1021 cagaaacgcc agcgcctggg cccggtccgc ctcgtccgcc gtgatcgagc cgaggacgga
     1081 catgacgagg aggaggaagg gaaagaggca cagcaccccg tagaaggcga tcgaggctgc
     1141 gtgtgtgaga tcgttgccct tgacgagccc gaccgcggcc cgccaggccg cctggccgga
     1201 gagacgcaac tccagccgca gccggcgcca tgcacgtcgc ccagccacaa tggatcagcg
     1261 gcgtgccgcc gcctactgct ggccgccccc cgcccgtcgg gcggccgccc ggccgtcggt
     1321 gatctcgtgc ttcttcatct tgctgtacag cgtggggcgg tacaagccca ggatgtgcgc
     1381 ggcctcctgc ttgttccagt tggtccgctg cagcgtctgc acgatcgcca tgcgctcgat
     1441 ttcggccagc gtccggtgcg gcggaatcgt gaagtcgtgc gtgccagcca ctgtctcccg
     1501 gatcgactcg ggcagatccg agggcgtgat ctctccgctc ttgcacacca gcaccgaccg
     1561 ctcgatcgca ttctccagct cgcggacgtt gcccggccag cggttccgaa tcagcaagtg
     1621 atagacggcc ggcgagatgg accgcacgga cttctggtac cgctgcctga acttcgcgag
     1681 gaagtgattg cagaggagag ggatgtcctc ggtccgctcg cgaagcggcg cgacccgcag
     1741 cgtgatcgtg ttgatgcgga agtacagatc ttcccgcagc cggccctccc gaagggccac
     1801 gtcgagatcc acgtttgtcg cgcagatgag acgaaaatcg accgtcacga gccggtcgcc
     1861 gccaacgggc ctgtaggcac gctcctgcag gacgcgcagc agcttcgtct gcaggtaccc
     1921 cggcatctcg ccgatttcgt ccagcagcag cgagcctcct tcggccatct cgaccaggcc
     1981 gaccttgtcg gaggtggcgc cggtgaaggc gcccttcttg tggccgaaca gctcgctttc
     2041 gatcagatcc ttgggaatgg acgcgcagtt gatcttgatg aaggggccgc ccgcgcggtg
     2101 cgagttgtag tgaatagcgt tggcgatgag ctccttgccg gtcccgttct cgccctggat
     2161 caggatgttg gcgtcgctgc gcgcgacgct ttcgaccagc tcgaagagct ccgtcatcgc
     2221 cttgctctgc ccgatgacgt tggcgaagct gtactgcccg cgcacccgct ccttgagctg
     2281 ctgatgctcg acccgttcgt cgatccgcgc ggccgccttg ccgagcagat ccagcagccc
     2341 ctcttcggaa accggccgtt caagaaagaa gaaggctcca gctcggccgg cctcgacggc
     2401 gcgcgtgatc gtgccctgcc cgccgatgac gatcacctcg ggctcgggat cgacgcgctt
     2461 gagtcgctgg agaagctcga tgccgtcgcc gtcgggcggg gccaggtccg tcaggacgat
     2521 gtggggacgc cactgccggt aaatctcttc accttgcagg cccgagatcg ctccacgcac
     2581 gtcgaacggc tccgactcga ccactcgctc gagccagggg aacatgccac tgtcgccgtc
     2641 aacgacgagc atccggcggc gccgtctcag ctgggggtgc actgaaatga cgaaatcctc
     2701 tgatcggtag actatcacgc gacgtctgca atcaatacac gacgggcgag cgcgactgaa
     2761 aagcagacag gcctgtaata tccgcctacc tcacggaccc gctgatctac tcctataatc
     2821 gcgaacatct catgcaggtc ctcgagaccc acgtccggcc cgaagctcag ctgtttcagg
     2881 acaatgcgcg acggatgagc accctcgtcg aagagcttcg cgcccgcctg gcgacggcgc
     2941 gcgcgggcgg cggcgagcga tatctccagc ggcaccgcga gctcggaaag ctccccgttc
     3001 ggacccggat cgaccggctc ctcgataccg gctcgccgtt tctcgagctc tcccctctcg
     3061 cggcctggga cgtgtacgac aacgacgctc ccggcgccgg catcgtcacc ggcatcggcc
     3121 gcgtgtcgtc gaaggaggtg ctcatcgtcg ccaacgacgc gacggtcaag ggcggcacct
     3181 actacccggc gaccgtcaag aagcacctga gggcgcagca cgtggcgctc gagaaccagc
     3241 tcccgtgcat ttacctggtc gattcgggcg gcgcattcct cccccttcag gcggaggtct
     3301 ttccggatcg cgatcatttc gggcgcatct tcttcaacca ggcgcggatg tccgcactgg
     3361 gcattccaca gatcgccgtc gtgatgggct cctgcactgc cggaggcgcg tacgtgcccg
     3421 cgatgtcgga cgagacgatc atcgttcagg gcacgggcac gatcttcctc ggcgggccac
     3481 cgctggtgaa agcagccacg ggcgaagaag tcacggccga ggaactgggc ggtgccgacg
     3541 tccacacgcg cctgtcgggc gttgccgatt atcttgccga cgacgacgac cacgcgctgc
     3601 gcctcgcgcg gacgatcgtc ggcacgctca atcgcgtcaa gacgatgccc gccgacatga
     3661 cctcgcctga ggaccctgcc tacgacccca gggagatcta cggcatcgtc aacgcggacg
     3721 tgcgcaagcc gtacgacgtc cgcgaggtca tcgcccggct cgtggacggc tcgcgattcg
     3781 acgagttcaa gcagcggtac gcgccgacga tcgtgaccgg tttcgcccgg ctgcacggct
     3841 tcctcgtggg catcgtcgcc aacaacggcg tgctgttttc ggagtcggcg ctcaaggcga
     3901 cccacttcat cgagttgtgc aacatgcgct cggttccgct gatcttcctg cagaacatca
     3961 ccggcttcat ggtgggccgc cagtacgagc gcggggggat cgcgaaagac ggcgccaaac
     4021 tggtccacgc cgtcgccaac tccgtcgtcc cgaagttcac cgtcgtgatc ggcgggtcgt
     4081 tcggcgccgg caactacggc atgtgcgggc gtgcctacga cccgcgtctc ctgtggatgt
     4141 ggccgaacgc acggatctcc gtcatggggg gcgagcaggc ggccggcgtc ctcacggcgg
     4201 tcaagcgcga gcagctcggc cgcgagggaa agtcgttcag tgccgaggag gagcaggcca
     4261 ttcgcggtcc tctcctcgag aaatacgagc acgaaggatc gccctactac tccacggcca
     4321 ggctctggga cgacgggatt ctcgacccgg ccgagacgcg ccaggcgctg gcgctcggca
     4381 tctcggcagc gtacaacgcg ccgataccgg cagcgaagta cggaatcttc cggatgtgag
     4441 tggccgcgtg agccacgatc acggacgcta accacagagg cacggagact cagaggccat
     4501 ttattctctg tgtcctctgt gactctgtgg ttaacgttcg tgggggaagg aaggatagga
     4561 gtgaccgaga gcacttcctt cagtcgcatt ctcgtcgcca accgcggcga gattgcggtc
     4621 cggatcatcc gggcgtgccg cgagatgggc atcgagtcgg tcgccgtcta ttcggacgcc
     4681 gacgctcgcg cgctccatgc gtcgctggcg gacgtcgcgg tcgggattgg accggcgccg
     4741 gctgccgaga gctacctgtc cattgacgcg atcctcgccg cggcggcgac gaccggctcg
     4801 caggcgatcc atcccggcta cggtttcctc tcacagagca cgcgctttgc ctccgcatgc
     4861 caggccgccg ggctcgtctt catcggacca ccgcccgagg cgatggcgcg catgggctcg
     4921 aagattgccg ccaggcgtct cgcgcacgac gccggtgtgc ccgtggtccc gggggagatt
     4981 cccggtgacc agtctgacca ggcgatcgcc gacgcggcgc ggcgcatcgg cttccccgtt
     5041 ctgctgaagc cctccgaggg gggcggcggc atcgggatga agacggcccg cgacgcgcgt
     5101 gaactggacg ccctcatcgg acaggcgcgg cgcgaagcgc aggcagcgtt cggcgc
//
LOCUS       DBNGSH010001016         6795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1846402, whole genome shotgun sequence.
ACCESSION   DBNGSH010001016 DBNGSH010000000
VERSION     DBNGSH010001016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1846402"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="AC16CY_23110"
     CDS             <1..576
                     /locus_tag="AC16CY_23110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356761.1"
                     /translation="PAYDFSAISTRIEEALEQSKYDAPESEIERPARLVLLATCRTLL
                     LEAGKLQEVASVPTTSLVRFGEELPADDRKWRSRARRWNGDELPWLSSIQLAWGASTF
                     PTLRLLNISSTGLLLESSVPLTQGNKAGFRLAGSDHEQLIVHARVVRADPLMEDRSET
                     KYVIAATFDWPFSSLGPVRSLRDLRRLDRIL"
     gene            complement(623..2974)
                     /locus_tag="AC16CY_23115"
     CDS             complement(623..2974)
                     /locus_tag="AC16CY_23115"
                     /inference="COORDINATES: protein motif:HMM:NF045061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3356762.1"
                     /translation="MPTNWIRLLTCVALLPLLVSGCGRRAADEAAATTTAAAKGTPGL
                     VGPLTNDTSDDIVSWVDGKVVSDWAAEIAKRDEAIEGYLNDADPARAAKYGFRSGQNP
                     ALAWSWFRDYPVGFNGVPFVVFKTLLDLDPNDPDPSLQRIARIWKRQTTMPIGSGPSA
                     AWTFDHIGIGPNPSDYADGVARPAGERRSPLPYGLVFENPRSFEPLSAIELTRHDAQL
                     RLKSVFQITSLLLAKLRTVDQEETWEDDRKGFGTPGSMDRVFFSCAACHVGRVVVGGK
                     MKFLPGMPNTEIEAQYYSKLLMLTSAALVESGFDPTSTTPVNPASISPNRNAVRALYG
                     AMLKKALDRPETMYGSSPADIARAKIQTLAVADRFPDVMKDLIAIGVKTHFIYYVVAK
                     NNGYKRPLPDVLEDRPGQMDAFGIASGLVAIHTRRTKNTFLEFVKNDNPKSPIFTGFS
                     KANGLPTDTPGMTEPVTDPKLAGDRIFKNIPAWAPPVPAPVDVKSVNWAVERYHANWD
                     GNQGASSRTLASGASATGDPRMVNVRIHEPLNPFIDNLPPPPYPFTSVDLARAREGKT
                     LFRANCAGCHMSRNQMIYPAATLGVDPNRTMVNTSVSRYGLAALVTEACGIYGENNKG
                     QPGADWCVPQGDWQARLDEYFRDTPRRVAEGTAGYKADMLHGIWAQAPYLHNGSVPTL
                     GQLVCPSTRPPRFLRGSLHYDEALVGFEWANRPTTRYGPNEMLLVKEFDTTVPGKSNA
                     GHAFGSDLCPDTSGLDPTSDRKEIEARLLESPVGALLAYLKTL"
     gene            complement(2976..4817)
                     /locus_tag="AC16CY_23120"
     CDS             complement(2976..4817)
                     /locus_tag="AC16CY_23120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR3356763.1"
                     /translation="MRLSVDDHVYLPPAVAAPPAPAAPVVPADPTAPEISFLATQTAV
                     ALQSFYATIERITADNPVSIARLQELAGKARLGQIAPEEAQELQGLKLQAVRQGRAVA
                     AVIGFFLKLKPYPMLEEVRKKAKLFQPAFGPVLVVEGDAVRNLLERDQEFSVDPYGVE
                     MVKVMTPSRNGGFTTFILSTDDNAAYEPDKRLLSAVCNREDADRITDTIHKDCVRRVG
                     AALASARSAGSSTIDIVKAVARFVPVTLGHRYLGVPVAALRGSFELTPEMLACYGSPI
                     EGQVETALRKDDGVIPDEEQMYLWIKAAFRHFFNNVQKDPQVQKDGLRACRLLLAYLL
                     REIGIQRDRILGGQPVDDTMLTRLLLFQLGRSAPTVARPADLDARLVSDLRIAENVMG
                     TIVGAIAGQEEATCRVIDSLMRLKGGEYRTSGSSSARYGSFSEATELAVNVLRGTAVA
                     ESRRTLYKYFLEALRLQPQGEVLARKCVSEGARIADSRPIAAGTFVFAAHGSAMRDVP
                     DADAFILDRPREHYLHYGWNRHTCLGQHVSPVIIVESMIAVLGLQDLSRPDPRAGESA
                     FPFERRFGRLQLDDQNLYATTFSLRFADTGATVSFWPPASAGGAEVT"
     gene            4943..5935
                     /locus_tag="AC16CY_23125"
     CDS             4943..5935
                     /locus_tag="AC16CY_23125"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3356764.1"
                     /translation="MDTSVLLTGASGFLGYHVAKKLNESGVRPRVLELRDSRTDVLNA
                     LNVERCSGALDDAASVRAACSGVGTLLHLAFKVSVGGGATLREEMERVNVAGTRQLLE
                     TAAASGVRRAVVAGSALAVGVNKQPQPLDESADWAEHAFNLPYALNRRKAELDALALA
                     SPSFAVMTVCPSFTLGPDDPVGAPANKLVGAVVSKKLRFTLPVGFGALDVRDFASGVL
                     LAAERGRSGQRYLLSGDNVTVNQLFEQVAGIAGVRAPRLVLPTPLVRVLVGVLQLVSS
                     IRGKPAPVTQDVLQIIGRYAWYDTSKARTELGWSSRPLRETLTDTIRWGQRERG"
     gene            5942..>6795
                     /locus_tag="AC16CY_23130"
     CDS             5942..>6795
                     /locus_tag="AC16CY_23130"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3356765.1"
                     /translation="MADIFISYASDDRDRVRPLAVALEAQGWSVWWDRHIVAGQAYDE
                     VIERELRSAKSVVTLWSASSTASEWVRNEAALAAERGVLVPALIDRVDLPLEFRRKQT
                     VDLVGWDGGTSHEGFQGLCRGIAASISGTAPPPRQTPGTPGPEPRTKWIAAASVAAAV
                     VVAAVAYWGWPREQPQPPPPPVAAEANPRGVEQPPSPSPNVEASTAPAAPKVTDRGPL
                     EAAIDSDVVRVRWTGRSVPLPERGTESRNELRLPVSFQLRGPAVVVRRVRVVTESGGE
                     MTTWDGTF"
BASE COUNT         1100 a         2211 c         2302 g         1182 t
ORIGIN      
        1 cccgcctacg atttcagcgc catctcgacg cgaatcgaag aggcgctcga gcaatcgaaa
       61 tacgacgccc ctgaatcgga gatcgagcgc ccggccagac tcgtgcttct cgcgacatgc
      121 aggacgcttc tgctcgaagc gggcaagctc caggaggtcg cgtccgtgcc gaccacgagc
      181 ctcgtgcgct ttggtgaaga gctcccggcc gacgaccgaa agtggcggtc ccgcgcgcgc
      241 cgctggaacg gtgacgaact gccctggctt tcgagcattc agctcgcgtg gggagcgagc
      301 acgttcccga cgctgcgtct tctcaacatc tcttcgaccg ggcttctcct tgaatcgagt
      361 gtccctctga cgcaggggaa caaagccggc tttcggcttg ccggctcgga ccacgagcag
      421 ttgatcgttc acgccagagt ggttcgcgcc gacccgctga tggaggaccg atctgagacg
      481 aagtacgtca tcgcggccac gttcgattgg ccattctctt cgctgggccc cgtgcgctcg
      541 ttgcgcgact tacgccggct cgatcgcatc ttgtgaccgg cggcgccgat tggcgttatc
      601 gatgtggtca ggctgaaccg gcttacagcg tcttcaggta ggccagcagc gctcccacgg
      661 gcgattcgag aagcctggct tcgatctctt tccggtcgct cgtcggatcg aggccgctcg
      721 tgtcggggca cagatccgac ccgaaggcat gaccggcgtt ggacttgccg ggcaccgtcg
      781 tgtcgaactc cttgaccagc agcatctcgt tgggtccgta tcgcgtcgtc ggccggttcg
      841 cccactcgaa cccgaccagc gcctcgtcgt agtgcaggct gccgcgaagg aatcgcggcg
      901 gacgcgtgga cgggcacacc aactggccga gcgtcggaac cgagccgttg tggaggtacg
      961 gcgcctgggc ccagatgccg tgcagcatgt cggccttgta tccggcggtg ccctcggcga
     1021 cgcgccgcgg cgtatcgcgg aagtactcgt cgagcctcgc ctgccagtcg ccctgcggga
     1081 cgcaccagtc ggcgcccggc tgccccttgt tgttctcacc gtagatgccg cacgcctcgg
     1141 tcaccagggc ggcgaggccg tagcgggaca cgctcgtgtt caccatcgtg cggttggggt
     1201 ccacgcccag cgtcgcggcc ggatagatca tctgattgcg cgacatgtgg cagccggcgc
     1261 aattcgcccg gaagagcgtt ttgccttcgc gcgcgcgggc cagatcgacg ctcgtaaacg
     1321 ggtacggcgg cggcggaagg ttgtcgatga acgggttgag cggttcgtgg atgcgcacgt
     1381 tcaccatccg cggatcgccg gtggccgacg cgcccgacgc gagcgttcgc gacgatgccc
     1441 cctgattgcc gtcccagttg gcgtgatagc gctcgacggc ccagttgacg ctcttgacgt
     1501 ccaccggcgc cggcaccgga ggtgcccacg ccggaatgtt cttgaagatg cggtcgcccg
     1561 caagcttcgg atcggtgacc ggttcggtca ttccgggagt gtcggtcggc agtccattgg
     1621 ccttcgagaa cccggtgaag atcgggctct tcgggttgtc gttcttcacg aactcgagga
     1681 acgtgttctt ggtccggcgc gtgtgaatcg cgaccaggcc agacgcgatg ccgaacgcat
     1741 ccatctggcc gggacgatcc tcgaggacgt cgggaagcgg ccgtttgtag ccgttgttct
     1801 tcgcgacgac gtagtagatg aagtgcgttt tgacgccgat cgcgatgagg tccttcatga
     1861 cgtccgggaa tcgatcggcg acggcaagcg tctgaatctt cgcgcgggcg atgtctgccg
     1921 gcgacgaccc gtacatcgtc tccgggcggt cgagcgcctt tttcagcatc gcgccgtaca
     1981 gcgcgcgaac ggcgttccgg ttcggcgaga tgctggccgg gttcaccggc gtcgtcgacg
     2041 tcggatcgaa gccggactcg acgagcgccg cagaggtgag catcagcagc ttcgagtagt
     2101 actgcgcctc gatctccgta ttcggcatgc cgggaaggaa cttcatcttc ccgccgacga
     2161 ccacgcgccc gacgtggcat gccgcgcacg agaagaacac gcgatccatg gacccgggag
     2221 tgccgaagcc tttgcgatcg tcttcccagg tctcctcctg gtccacggtc ctgagcttgg
     2281 cgaggagcag gctcgtgatc tgaaacacgc tcttcaggcg cagctgcgcg tcatgacgcg
     2341 tcagctcgat cgctgacagc ggctcgaacg accgcgggtt ctcgaagacc aatccgtagg
     2401 gcagcggcga ccggcgctcg ccggcgggcc tggccactcc gtcggcgtag tcggaaggat
     2461 tcggtccgat gccgatgtga tcgaaggtcc acgcggcgga aggccccgat ccgatcggca
     2521 tcgtggtctg gcgcttccag atccgggcga tccgctgcag ggaggggtcc gggtcgttcg
     2581 gatccaggtc caggagcgtc ttgaagacca cgaacggtac gccgttgaag ccgaccgggt
     2641 agtcgcggaa ccagctccac gccagcgccg ggttctggcc gctgcggaag ccgtacttcg
     2701 cggcgcgggc gggatcggcg tcgttcaaat agccttcaat cgcctcgtct cgtttcgcga
     2761 tctccgctgc ccagtccgag acgaccttcc cgtccaccca cgacacgatg tcgtcgctcg
     2821 tgtcattggt caacgggccg acgagtccgg gagttccttt cgcggctgca gtcgtcgttg
     2881 ccgccgcctc gtcggcagcc cgccggccgc acccggagac aaggagcggc agcagcgcaa
     2941 cgcacgtcag cagtcggatc cagttggtcg gcatctcacg tcacctcggc gccgccggcc
     3001 gatgcagggg gccagaacga gaccgttgca ccggtgtcgg caaaccggag agagaaggtc
     3061 gtggcgtaca ggttctggtc gtcgagttgg agccggccga accgccgttc gaacgggaag
     3121 gccgattcgc cggcccgagg atcgggccgg gacagatcct gcaggccgag aacggcgatc
     3181 atcgattcga cgatgatgac cggcgagacg tgctgaccga ggcacgtgtg ccggttccag
     3241 ccgtagtgca agtagtgctc gcgaggcctg tcgaggatga aggcgtccgc gtctggaacg
     3301 tctctcatcg ccgacccgtg cgccgcgaag acgaacgtac cggcggcaat cggacggctg
     3361 tcggcaatgc gcgctccttc gctgacacac tttcgcgcca gcacttcgcc ctgcggctgc
     3421 aggcgaaggg cctccaggaa gtacttgtag agcgtgcgcc ggctctccgc cacggcggtg
     3481 cccctcaaca cgttgactgc cagttcggtg gcctcactga agctgccgta tcgggccgat
     3541 gaggagccgc tcgtccggta ctcgccgccc ttcaaccgca tgagcgagtc gatcacccgg
     3601 cacgtcgcct cttcctggcc cgcgatggcg ccaacgatcg ttcccatgac gttctcggcg
     3661 atccgcagat cgctgaccag ccgtgcgtcg agatccgccg ggcgggcgac ggtgggggct
     3721 gaacgaccga gctgaaagag gaggagcctg gtgagcatcg tatcgtccac cggctgaccg
     3781 cccaggatgc ggtctcgctg gatgccgatc tcgcgcagga ggtaggcgag cagcaggcgg
     3841 cacgcgcgca acccgtcctt ctgcacctgc ggatccttct gtacgttgtt gaagaagtgc
     3901 cggaaggcgg ccttgatcca cagatacatc tgctcttcgt cggggatgac gccgtcgtcc
     3961 ttcctcagcg cggtctcgac ctggccctcg atcggcgacc cgtagcaggc cagcatctcc
     4021 ggagtgagct cgaaggaacc acgcagcgcc gccacgggaa cgcccagata cctgtgcccg
     4081 agcgtgaccg ggacaaagcg cgcgacggcc ttcacgatgt cgatcgttga cgagccggcg
     4141 ctccgggcgg acgcgagcgc ggcacccaca cgtcgaacgc agtccttgtg gatcgtgtca
     4201 gtgatccggt ccgcatcctc gcggttgcac acggccgaga gcagacgctt gtcgggctcg
     4261 taggcagcgt tgtcgtccgt gctcaggatg aacgtggtga atccgccgtt gcgcgacggc
     4321 gtcatcacct tcaccatctc caccccgtag ggatcgacgc tgaactcctg gtcgcgttcg
     4381 agcaggttgc gaacggcgtc tccttcgacc accagcaccg gaccgaacgc cggttgaaac
     4441 agcttcgcct tcttcctgac ctcttccagc atcggatacg gcttcagttt caggaagaag
     4501 ccgatgaccg cggccaccgc gcgaccctga cgaactgcct ggagcttcaa tccctggagc
     4561 tcctgcgctt cctccggagc gatctgaccg agccgggctt tcccggcaag ctcctggagt
     4621 ctggcgatcg agaccgggtt gtcggccgtg atccgctcga tcgtcgcgta gaaagactgc
     4681 agcgccacgg cggtctgcgt ggcgagaaag gagatctcgg gggcagtagg atccgccgga
     4741 acgacggggg cagcgggagc gggaggagca gctaccgccg gcggtaggta cacgtggtca
     4801 tcgacggaga gacgcatgaa gaacctcagc cagctgcggt gggcccattc tcactggctg
     4861 ttcttgccga acacaagtgc aaatcggacc aatggatgag attgatgtat gccttattgt
     4921 gtgtgccgga ggatttcagc cagtggacac cagcgtcctg cttacgggag cgtcggggtt
     4981 tctcgggtac cacgtcgcga agaagctgaa cgagtccggc gtgcgcccgc gggtgctcga
     5041 gctgcgagac agccggaccg acgtgctcaa cgcgctgaac gtcgaacggt gttccggtgc
     5101 cctggacgac gcggcgtcgg tgcgcgccgc ctgctccggc gtcgggacgt tgctgcacct
     5161 tgcgttcaag gtgagcgtcg gaggcggcgc gacgcttcgc gaagagatgg aacgagtcaa
     5221 cgtcgcgggt acgaggcagc tgctggagac cgcggcagcc agcggcgtca ggcgcgccgt
     5281 cgtcgcgggc agcgcgctgg ccgtcggcgt caacaagcag ccacagccgc tcgacgaatc
     5341 cgccgactgg gccgagcacg ccttcaatct tccgtacgcc ctcaatcggc ggaaggcgga
     5401 gctggacgcc ctggcgctgg cgtcgccgtc gttcgccgtc atgacggtgt gcccgtcatt
     5461 tacactgggc ccggatgatc cggtgggcgc gccggccaac aagctggtgg gagcagtggt
     5521 ttcgaagaaa ctgcgcttca cgctccccgt cgggttcggg gcgctcgacg tccgggactt
     5581 cgcgtcaggt gtcctgcttg ctgccgagcg tggccgatcg ggacagcgat acctgctgag
     5641 cggcgacaac gtcacggtga accagttgtt cgagcaggtg gccgggatcg cgggtgtccg
     5701 cgcacctcgc ctcgtccttc ccacacccct cgtgcgcgtg ctggtcggcg ttctccagct
     5761 cgtcagctcg atccgcggca agccggctcc cgtgacccag gacgtcctgc agatcatcgg
     5821 ccgctacgcg tggtacgaca cctcgaaggc gcgaaccgag ctcggatggt cgtcgcggcc
     5881 cctgcgcgag acgctgaccg acaccattcg atggggccag agagagaggg gctgagctcg
     5941 catggcggac atcttcatca gctacgcgag cgacgatcgc gaccgggtgc ggccgctggc
     6001 cgtcgcgctc gaggcgcagg ggtggtcggt ctggtgggat cgccacatcg tggccggcca
     6061 ggcctacgac gaggtcatcg aacgcgagct gcggtcggcg aaaagtgtcg tcacgttgtg
     6121 gtccgccagc tcgacggcgt cggaatgggt gcggaacgag gctgcgcttg cggccgagcg
     6181 aggcgtcctc gtgccggccc tcatcgaccg cgtcgatctg ccgctggaat tccggcgcaa
     6241 gcagaccgtc gatctggtgg gttgggacgg cggaacgtcg cacgagggat tccaggggct
     6301 ctgccgagga attgcggcgt cgatttctgg aaccgcgccc ccgccacgcc agacgcccgg
     6361 cactccgggg ccagagcctc gcacgaagtg gatcgcagcc gcaagcgtag ccgccgcggt
     6421 ggtcgtcgcg gccgtagcgt attggggctg gccgcgcgaa cagccgcagc cgccgccgcc
     6481 accggttgcc gcagaagcca atccgcgtgg cgtggagcag ccgccttcgc cctctccgaa
     6541 cgtcgaggcc tctactgcgc ccgccgcgcc caaagtcact gaccggggcc ccctcgaggc
     6601 cgcgatcgat tccgacgtcg tgcgcgtgcg atggactggc aggagtgttc cgctccccga
     6661 gcggggcacc gaatcgcgga acgagctgcg gcttccggtc tcgtttcagc tccgcggacc
     6721 cgctgtcgtc gtccgccgcg tccgcgtggt cacggagtcg ggcggcgaga tgacgacctg
     6781 ggatgggact ttcga
//
LOCUS       DBNGSH010001017         1501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2865551, whole genome shotgun sequence.
ACCESSION   DBNGSH010001017 DBNGSH010000000
VERSION     DBNGSH010001017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2865551"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="AC16CY_23135"
     CDS             <1..803
                     /locus_tag="AC16CY_23135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF045201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ family protein"
                     /protein_id="HIR3356766.1"
                     /translation="ANCLRACARHGVPVIVCDRPNPIGGLDVEGALLSPGYESFVGQF
                     AIPMRHGMTIGELGRLFNEHFGLGADLEVVRMDGWTRGMYADATGLPWVMPSPNMPTL
                     DTAIVYPGTVLFEGILLSEGRGTTRPFELVGAPWIDADRFAARMNDLALPGVHFRPAV
                     FEPTFQKHAKQSCGGCQIHVTARDVFRPVLVAVALAGMFRQADPSRFAWRQPPYEYEH
                     VKMPIDILAGSDTLRRQIEAARPALEIAAGWREDEDAFRTLRKQYLMY"
     gene            851..1315
                     /locus_tag="AC16CY_23140"
     CDS             851..1315
                     /locus_tag="AC16CY_23140"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3356767.1"
                     /translation="MREFTHSLTIDAPAAAVLDAFFDADSLAAWWSVSRSLCVARPLG
                     VYAVEWTPTEWEDDILGRLGGAFRGTVIEFKPGREFFVADLFWLPPDGEPIGPMALEA
                     ACSQHGNSTVLHVRQSGWEDSPRWSRYYDLVATGFALALSQLKRHVEEKWKD"
     gene            complement(1320..>1501)
                     /locus_tag="AC16CY_23145"
     CDS             complement(1320..>1501)
                     /locus_tag="AC16CY_23145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356768.1"
                     /translation="WGVQPLLTDFSGDVGSAAARITADLIARGVLEPGSIVVVVNVTT
                     ELSPGSASFLRLQRV"
BASE COUNT          230 a          495 c          530 g          246 t
ORIGIN      
        1 tggcgaactg ccttcgcgcg tgcgcgcgcc acggcgtccc ggtcatcgtg tgcgaccggc
       61 cgaatccgat tggcggcctc gacgtggaag gggcgctgct ctcgccgggc tacgagtcct
      121 tcgtggggca gttcgcgatt cccatgcgtc acgggatgac gatcggcgag ctcggacggc
      181 tgttcaacga acacttcggc ctcggcgccg acctcgaagt cgtgaggatg gacggctgga
      241 cacgcgggat gtacgccgac gcgacgggac tgccgtgggt gatgccgtcg ccgaacatgc
      301 ccacgctcga cacggcgatc gtgtacccgg gaacggtgct cttcgaaggc atcctcctct
      361 cggaggggcg cggaaccacg cggccgttcg agctggtggg cgccccgtgg atcgacgcgg
      421 accgtttcgc ggcccgcatg aacgacctcg cgctgcccgg ggtccatttc aggcccgcgg
      481 tattcgagcc gacgttccag aagcatgcga aacagagctg cggcggctgc cagattcacg
      541 tgacggcccg cgacgtgttc cgccccgtgc tggtcgccgt cgcgctcgca ggaatgttcc
      601 gccaggccga cccgtccagg tttgcctggc ggcagccgcc gtacgagtac gagcatgtca
      661 agatgccgat cgacatcctg gcgggctccg acacgctgcg ccggcagatc gaggccgcgc
      721 ggcccgctct cgagattgcg gcgggctggc gcgaggacga ggacgcgttt cgaacgctcc
      781 ggaagcagta cctgatgtac tgaacgcgca cgctgaagcg tgcgctctac gggcggccag
      841 gcgaagacca atgcgcgagt tcacacacag cctcacgatc gatgcgccgg cggccgccgt
      901 gctcgacgcc ttcttcgacg ccgattcgct ggccgcgtgg tggagcgtct cccgctcgct
      961 gtgtgtggcc cggccgctcg gcgtgtacgc cgtggagtgg acgccgaccg agtgggagga
     1021 cgacatcctg ggccggctcg ggggcgcctt tcgcgggacc gtgatcgagt tcaagccggg
     1081 acgggagttc ttcgtcgccg acctcttctg gctgcctccg gatggcgaac cgatcggtcc
     1141 gatggcgctg gaggcggcgt gcagccagca tggcaacagc accgttcttc acgtccgcca
     1201 gtctggctgg gaggacagtc cgcggtggtc gcgctactac gacctggtcg cgaccggctt
     1261 cgcgctggcg ctctcgcagc tcaagcggca cgtcgaggag aagtggaagg attgaacctt
     1321 cacacccgct gaagcctcag gaagctcgcg gacccggggg acagttcggt cgtcacgttg
     1381 acgaccacga cgatcgagcc gggctcgagt actcctcgtg cgatgagatc cgcggtgatg
     1441 cgggcggctg ccgagccgac gtcgccgctg aagtcggtca gcagcggctg cactccccag
     1501 a
//
LOCUS       DBNGSH010001018         2774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_659054, whole genome shotgun sequence.
ACCESSION   DBNGSH010001018 DBNGSH010000000
VERSION     DBNGSH010001018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_659054"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="AC16CY_23150"
     CDS             complement(<1..748)
                     /locus_tag="AC16CY_23150"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HIR3356769.1"
                     /translation="MQGHAAIRPVRPLLKWAGGKRQLLPVLAEHYPSTFSRYIEPFVG
                     SGAVFFDLLNSGRLTRSEILLCDVNPDLIGCYRMVRDQTDEVIRALKTLAREYRDSGH
                     RCYYDVRDRRFNPARAELQMRSSGPEVASRYTPELAAMLIFLNRTGFNGLFRLNRRGG
                     FNVPAGRYTDPRICDEGNIRAVAGALGREGVSINLSPFDETLAGAARGDFVYCDPPYA
                     PLSRTSSFAQYTADGFSSLDQQRLQRAVIGA"
     gene            complement(787..969)
                     /locus_tag="AC16CY_23155"
     CDS             complement(787..969)
                     /locus_tag="AC16CY_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008574708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin"
                     /protein_id="HIR3356770.1"
                     /translation="MADRKPFLLRIDARVLEGVQRWANDDLRSLNAQVEYLLREALRK
                     AGRAPKDPPMERPEDG"
     gene            complement(982..1872)
                     /locus_tag="AC16CY_23160"
     CDS             complement(982..1872)
                     /locus_tag="AC16CY_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3356771.1"
                     /translation="MIRERERNGLSAIPLLVLAILAPVGIVLLFAWGGAALDDGFNLG
                     GLFAVLVAIALVPTWIVSLKGFFMVAPNEAKVLQLFGDYVGTAKVTGLRYTNPFYGKR
                     KISLRVRNFESGQLKVNDHDGNPIDIAAVVVWRVVETAEASFEVDDYEHYVRVQSEAA
                     LRNAATNYPYDAHEDGVVSLRGSTAVVAEHLKKEVQQRLEKAGVEVLEARFSHLAYAP
                     EIAAAMLQRQQAGAIIAARQKIVEGAVGMVEMALAQLSTKNIVHLDDERKAAMVSNLL
                     VVLCGERAAQPIVNTGTIHQ"
     gene            2016..>2774
                     /locus_tag="AC16CY_23165"
     CDS             2016..>2774
                     /locus_tag="AC16CY_23165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356772.1"
                     /translation="MHSSRLVLLLLLVTAATSSAGPQTVPLPPLPVTRLDARQTDPEL
                     DGRRMSFGFPQPTPVRDVLLMLVRGARVSLIPQTTIEQTFVGDLKNVTLREALDLILE
                     PLGLDYSVRGQVIRVFPRELETRLYSVDYVSAQRSVRSRAATAVTGGPNVAPDLYDDL
                     AEGVRSLLSPGGGMNLDRTAALLQVSDSPSRLARIENYLETVMLRATRQVQIEATVIE
                     VELSDQFSAGIDWPAVLVKPNTPVGASTLLKALAG"
BASE COUNT          467 a          919 c          892 g          496 t
ORIGIN      
        1 acgcgccgat gacggcccgc tgcagtcgct gctgatcaag agaggagaac ccgtccgccg
       61 tgtactgcgc gaagctggac gtgcggctca gcggagcgta cggggggtcg cagtagacga
      121 agtctccgcg cgcggccccg gccagcgtct cgtcgaaggg cgagaggttg atggacacgc
      181 cctcccggcc aagcgcgccg gcgaccgcgc ggatgttccc ctcgtcgcag attcgcggat
      241 cggtgtatcg acctgcgggc acgttgaagc cgccgcggcg attcagccgg aacaagccgt
      301 tgaagccggt acgattcagg aagatcagca tcgccgccag ctcgggcgtg tagcgcgagg
      361 cgacctccgg gcccgatgac cgcatctgca actctgcgcg cgcgggattg aatcgtcgat
      421 cgcgcacgtc gtagtagcac cggtgccccg aatcgcggta ctcgcgggcg agcgtcttca
      481 acgcacgaat cacctcgtcg gtctggtcgc ggaccatccg ataacagccg atcaggtccg
      541 gattgacgtc gcacagcagg atctcgctgc gcgtcaggcg ccccgaattc aacaggtcga
      601 agaacaccgc tccgcttccg acgaacggct cgatgtagcg ggagaaggtg gaagggtagt
      661 gctcggccag gacgggcagc agttgacgct tgccgccggc ccacttgagg agcggccgaa
      721 ccggacgaat ggctgcatgc ccttgcacat gccaattatc ggtgctttgc gcccgggccg
      781 taggccctac ccgtcctccg gccgctccat cggcgggtcc ttcggggcgc ggcccgcctt
      841 tctcagcgcc tcgcggagca ggtattccac ctgggcgttc aggctgcgca ggtcgtcgtt
      901 ggcccagcgc tgaacgccct cgaggacccg ggcgtcgatg cgaagaagga agggcttccg
      961 atccgccacg gtccccttcg cctattgatg aatcgttccc gtgttgacga tcggctgcgc
     1021 cgctcgctcg ccacacagca ccacgagcag gttgctcacc atcgcggcct tccgctcgtc
     1081 gtccaggtga acgatgttct tcgtggaaag ttgcgcgagc gccatctcca ccatcccgac
     1141 cgcgccctcg acgatcttct ggcgggccgc gatgatcgcc ccggcctgtt gtcgctgcag
     1201 catggccgcc gcgatttcgg gcgcgtaggc caggtggctg aaacgggcct cgagcacctc
     1261 cacgccggcc ttctcgagcc gctgctggac ttccttcttc agatgttcgg caaccaccgc
     1321 ggtcgatccc ctgagcgaga cgacgccatc ctcgtgtgcg tcgtacgggt agttcgttgc
     1381 ggcgtttcgc agggccgctt cgctctgcac gcggacgtag tgctcgtaat catcaacctc
     1441 gaagctggcc tcggcggttt cgaccacccg ccagacgacg acagcggcga tgtcgatggg
     1501 gttgccgtcg tggtcgttga ccttgagctg cccgctttcg aagttccgga cgcggagcga
     1561 gatcttcctc ttgccgtaga acgggttggt gtagcgcagg cccgtcacct tggccgtgcc
     1621 gacatagtcg ccgaagagct gcagtacctt ggcctcgttg ggggccacca tgaagaaccc
     1681 cttcagcgac acgatccagg tggggaccag tgcaatggcg acaagcactg cgaataagcc
     1741 gccgaggttg aagccgtcgt caagggccgc gccaccccaa gcgaagagca agacgatgcc
     1801 cacaggggcc aggatcgcta ggaccaggag tgggatggcg gacaggccgt ttcgctcacg
     1861 ttcacggatc atcgacgacc tccaaaggaa gggcgtttcg cccagttgac atctaagtga
     1921 tatcactatg aaagcagcgc cgtcaaggcc ggaggtcccg gtcgggctat ttgccccaac
     1981 tcctcagtcc gacaatgagg accgagggaa agtctgtgca ctcgtctcga ctcgttctct
     2041 tgctgcttct tgtgacggcg gcgacctcgt ccgccgggcc gcagacggtg ccgcttccgc
     2101 cgctcccggt gacgcggctg gacgcgcggc agaccgatcc tgaactggac ggccggcgga
     2161 tgtccttcgg attccctcaa ccgaccccgg ttcgagacgt cctgctgatg ctcgtacgcg
     2221 gcgctcgagt gagcctgatt ccgcagacca cgatcgagca gacgttcgtt ggcgacctca
     2281 agaacgtgac gctccgcgag gcgctcgatc tcatcctcga gccgctcggc ctcgactatt
     2341 cggttcgcgg gcaggtgatc cgcgtgtttc cccgggagct cgagacgcgg ctgtacagcg
     2401 tcgactacgt gagcgctcag cgatccgtcc gttcgcgtgc cgcaacggcc gtaacgggcg
     2461 gcccgaacgt cgcgcccgat ctgtacgacg acctcgccga gggcgtccgc tcgctgttgt
     2521 cgcctggtgg aggcatgaat ctcgatcgca ctgcggcgct gctgcaggtc agcgacagcc
     2581 cgagccgcct cgcccggatc gagaactatc ttgaaaccgt gatgctgcgc gcgacccgtc
     2641 aggtgcagat cgaagccacg gtgatcgagg tcgagttgag cgatcagttt tccgccggca
     2701 tcgactggcc tgccgtcctt gtcaagccga acacgcctgt cggcgcttca acgctcctga
     2761 aagccctggc cggg
//
LOCUS       DBNGSH010001019         2184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4225516, whole genome shotgun sequence.
ACCESSION   DBNGSH010001019 DBNGSH010000000
VERSION     DBNGSH010001019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4225516"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="AC16CY_23170"
     CDS             complement(<1..346)
                     /locus_tag="AC16CY_23170"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3356773.1"
                     /translation="MLRFGRVIVLFALCVAAPAAAYAQASIVGTARDASGAVLPGVAV
                     EASSPALIEKTRTVVTNAVGQYAIEDLRPGTYTVTFTLSGFSGIRREGIELAGAFVAT
                     VNAEMRIGGVTET"
     gene            complement(607..1872)
                     /locus_tag="AC16CY_23175"
     CDS             complement(607..1872)
                     /locus_tag="AC16CY_23175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3356774.1"
                     /translation="MTTNSRFAGTFAVIVAAAGWGIGNAQAPATQGAKAARVSHQDWP
                     TGHYRVVENWPKPLPDTRHSHDGWTWGSFGGVYAESPDRIWIAMRGELPLPQGASPWT
                     PYAALNPSRGNATGNGDGISATCQAEPKRGWERRFQHSILVVDGQGNLVDEWPHLDEM
                     FSKLPCGRGPHQIKISPYDKEKHVWIIDDQLHMIYRFTYQGKLVHSKGELGKRGRGPN
                     TFDRPTDIAWLPDGTYFITDGYGGTRVAKYDAKDNFITDWGQAPKDPKSPGPNEFNTV
                     HSIAISADRRLFVVDRGHARMQVFDENGKFLDMWTLRSPHWPANQNTLMVNHFIDQSG
                     FIWVGDAPTSRIMKFDLNGNFLYGWGAPGGQAGRLNCSHGMTTDQLGNLYVADCFAGR
                     VQKFEPVPGADPTKIAGQILRTWDTWKRK"
     gene            complement(2017..>2184)
                     /locus_tag="AC16CY_23180"
     CDS             complement(2017..>2184)
                     /locus_tag="AC16CY_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR3356775.1"
                     /translation="KYGKTVTMLPKKLDEEVARLHLDQLGVKLTKLSKEQADYLGVPV
                     EGPYKPEHYRY"
BASE COUNT          371 a          722 c          675 g          416 t
ORIGIN      
        1 tcgtctccgt gacgcccccg atcctcatct ctgcgttgac ggtcgcgacg aacgcgccgg
       61 ccagttcgat cccttcgcgc ctgatcccgc tgaacccgga cagcgtaaac gtgaccgtat
      121 aggtcccggg cctcaggtcc tcgatggcgt actgaccgac cgcattggtc acgaccgttc
      181 gggtcttttc gattagcgcc gggctcgatg cttcaacagc gacaccgggc aacacggccc
      241 cggaggcatc cctggcggtc cccacaatcg aggcttgagc gtaggcggcc gccggcgcgg
      301 caacgcacaa cgcgaacaac acgatcaccc tgccaaatct caacatgtcg aaatcctcca
      361 cgaggagcga acgatcgttg tgagttgaga gaaagtcccc atgacttggc gctgatccgg
      421 cgacagccct aatccaatcc aaaatgctgg atagctgtgg ggagactgat atgccgccgg
      481 cctcgcaagt ggttcctcat cacgtcttta ctgtgtttca ccgatagcag cactcttcct
      541 ccaggcagag gggccgcatc atgattcaac agccgcgcca agcggcccga ctcggtcgag
      601 cctcggtcac ttgcgcttcc aggtatccca ggtccgcaga atctgtcccg cgatcttggt
      661 gggatcggcg ccgggaaccg gctcgaactt ctgcacccgg cctgcgaagc aatcggccac
      721 gtacaggttc ccgagttgat cggtcgtcat cccgtgcgag caattcagcc gcccagcctg
      781 cccgcccgga gcgccccagc cgtacaggaa attcccgttg agatcgaact tcatgatccg
      841 gctcgtcggc gcgtcgccca cccagatgaa gccgctctgg tcgatgaagt gattcaccat
      901 gagcgtgttc tggttggcgg gccagtgcgg ggaccgtagc gtccacatgt cgaggaactt
      961 gccgttctcg tcgaagacct gcatccgtgc atgtccccga tcgaccacga agagccgtcg
     1021 gtcggcgctg atcgcgatgc tgtgaacggt gttgaactcg ttcggccctg gactcttcgg
     1081 gtccttcggc gcctggcccc agtccgtgat gaagttgtcc ttggcgtcgt acttcgctac
     1141 gcgcgtcccg ccgtagccgt ccgtgatgaa gtacgtcccg tcaggcagcc acgcgatgtc
     1201 cgtcggccga tcgaacgtgt tcgggccgcg gccacgcttg ccgagctctc ccttgctgtg
     1261 tacgagcttg ccctggtagg tgaagcggta aatcatgtgg agctggtcgt cgatgatcca
     1321 cacgtgcttc tctttgtcgt aggggctgat cttgatctgg tgcggtccgc ggccgcacgg
     1381 cagcttgctg aacatctcgt cgaggtgcgg ccactcgtca acgaggttcc cctggccgtc
     1441 cacgacgagg atcgagtgct ggaaccggcg ctcccatccg cgcttgggct cggcctggca
     1501 ggtcgccgag atgccgtcgc cgttgcccgt ggcgttgcca cgcgacgggt tgagcgcggc
     1561 gtacggcgtc cagggcgagg ctccctgcgg cagcggcagc tcgccgcgca tcgcgatcca
     1621 gatgcgatcc ggactctccg cgtagacacc gccgaacgat ccccaggtcc agccgtcgtg
     1681 cgagtgccgc gtgtccggca gcggcttcgg ccagttctcg acaaccctgt aatggcccgt
     1741 cggccagtcc tggtgactca cacgcgcggc cttcgcacct tgagttgccg gcgcctgggc
     1801 gttgccgatg ccccagccgg ctgccgcaac gatcaccgcg aacgtccccg cgaaacgcga
     1861 attcgtagtc acaggcggtc ctttcgagtg tggcgagtgg ccggagtata gacgaaggca
     1921 gggggcaggg ggcagggggg cagggggcag gggcagagga aaagaaaaag ggccggcccg
     1981 aaaggaccgg ccccctcact aaccaccaac cactcactag tagcggtagt gctccggctt
     2041 gtacggtccc tcgacgggca cgccgaggta gtcggcctgt tccttcgaga gctttgtcaa
     2101 cttgacgccg agctgatcga ggtgcagccg cgccacttcc tcgtccagct tcttcggcag
     2161 catcgtcacc gtcttgccgt actt
//
LOCUS       DBNGSH010001020        12990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3715869, whole genome shotgun sequence.
ACCESSION   DBNGSH010001020 DBNGSH010000000
VERSION     DBNGSH010001020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 12990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12990
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3715869"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16CY_23185"
     CDS             complement(<1..230)
                     /locus_tag="AC16CY_23185"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3356776.1"
                     /translation="MVPALVLTAGLATRLRPLSYVRAKAALPLAGVPLVRRILRLLSA
                     AGVTDAVLNLHHLPHTLTTVVGDGSDLGLRVR"
     gene            complement(209..775)
                     /locus_tag="AC16CY_23190"
     CDS             complement(209..775)
                     /locus_tag="AC16CY_23190"
                     /inference="COORDINATES: protein motif:HMM:NF014038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HIR3356777.1"
                     /translation="MIEKEIKLMFGSPSEARAAILAAGAAPLRCRRLQEDAMFDTGDE
                     LLRRSGCALRVRSESGRSLLTFKGPVQPGVVKTREEHETVVSDGDVMTRVLESLGLHV
                     WFRYQKFREEFAAEDAMIALDETPIGTFVEIEGGERAIEQMARALGRSSSDYLLDSYR
                     GLFLRRREQFGFTGADMMFADEWSQRSS"
     gene            complement(911..2443)
                     /locus_tag="AC16CY_23195"
     CDS             complement(911..2443)
                     /locus_tag="AC16CY_23195"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3356778.1"
                     /translation="MSSRRQTWFYGLLIAVTSAAAALVFASRLGLAPESAAQTPAPAM
                     NSSPLNGPLTSSTFRDIAKTVSPAVVNIRTESRRRTQDLSEFFGGDSGLFERFFGQPD
                     SRDERQRPREELVRSAGTGFIIDKAGLILTNNHVVEGATKIKVSLYGEDEDQEYDARV
                     IGRDVLTDSALIELTQKPDHTLPEVRFGDSSQMQPGDWVMAIGNPFGLAHTVSVGVIS
                     GNRPSELEVAEGRRVSVIQTDAAINPGNSGGPLLNIRGEVVGVNVAIYTDARRQGNIG
                     IGFAIPANSVRELLPQLRVGKVTRGRIGVDVLSIPREAVDELGLKTRGGALIRRVLAD
                     SAASRAGLEPGDVIIAFGGKPVQARDELIQMVLNTKPGTTVPLRIVRNREERSLSITV
                     DELDLEAENRTERAAKPDADPAPETSSGFGFKLQDVTAEVARQLNLRDRRGAVVTAVE
                     PGGPAERLLAPGDLIVRVGGQQVQNRAEAQRELDQVRSGGTAFLRVIRGGEEIFVPVT
                     KE"
     gene            complement(2637..3818)
                     /locus_tag="AC16CY_23200"
     CDS             complement(2637..3818)
                     /locus_tag="AC16CY_23200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3356779.1"
                     /translation="MPIDVTAVQERLRQAGLDGWLLYDFHGSNPIATRLAGLSDGRHM
                     TTRRWYYLIPASGQPRGLVHAIERHNLDGLPGERLVYAGRQQLENGLTRLLSGMTRVA
                     MEYSPMCAIPYVSRVDAGTVEMVRARGVEVLSSGDLVQQFEARWTPEQLASHRDASAS
                     LYRIKDRAFQEAADALRRGRSLNEYQLQQQMVRWFEDEGLVSDSPPVVAVGGNAGDPH
                     YLPAAGDCRAIVANEVLLLDLWGKKPDPGAVFADITWVAVTSARVPGEVSKAFHAVAL
                     ARDAAVELVVGAARDGRDLHGWEVDRAARVVLADEGYADRIVHRTGHSLGENVHGNGA
                     HMDDYETHDDRQLLPGTGFTIEPGLYFESFGVRTEINVYRGEHEALVTGPRQSEVVTL
                     A"
     gene            3876..5963
                     /locus_tag="AC16CY_23205"
     CDS             3876..5963
                     /locus_tag="AC16CY_23205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3356780.1"
                     /translation="MTRRLALALIAAALGGVLPAAQQQAPAPTAVEAQAEVPQVTFRV
                     EVNYVEVDAIVTDGQGNPVTNLTKDDFELFEDGRRQDIASFSLVNIPIERAEQALFAT
                     TPIEADVQTNENVEGRIYLLVLDDLHTDASRTARVKAAARRFIEQNFGTNDLAAVVFT
                     WRSALGQDFTNNKRMLIAAIDKFNGGKLRSETLNRLDTASPNPQTGELQPGTDIDQMD
                     RAHRARQLAHSIKKLAEFMAGVRGRRKAMLLIGEGVDYDIYQVTGQLGATASSVVLDS
                     QDAIAAATRGNVTIYTIDPRGLMNAETELIEAQGPIGDAGIQSSQYSFRTSQDTLRAL
                     AGNTGGFASLNRNDFDTAFDRIVAENSSYYLLGFYSTNDRRDGRFRKLEVRARRPGLR
                     VRNRNGYFEARGRAPAQLLPSQSPNALPPAVSAAVGSPIPMAGLPIKVFAAPYKGTAP
                     NAAVALVFEIDIKGLNIVEKDGTFNEQLEVVYNTVNNNNRVFPGDRHTVTMAMKPETY
                     ERVKTHGLRVLSQAQLPPGRYQMRVAAGDRSGTAGSVVYDLEVPDFSSAPLVMSGVSL
                     TAASAGQTWTIKPKDPLGDVLPGPPTARREFDKADLVVLFAEFYENAPTARPHMLDFK
                     AELREQGGRVVQQIADERSSTELQGSSGGYGFTARFQLQDAQPGLHVIHVEGRSRDNP
                     DSVVSRDIQIRVR"
     gene            5980..7194
                     /gene="gltS"
                     /locus_tag="AC16CY_23210"
     CDS             5980..7194
                     /gene="gltS"
                     /locus_tag="AC16CY_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HIR3356781.1"
                     /translation="MRLDLVQTVAFAGVMLFAGYGLKRQIPLLARYNIPAPVVGGLPA
                     AALFTLLRALDLSGLEFDTTLQVPLQNTFFASIGFGASVLVLRRGGPLVLIMMVLASV
                     AAALQNVVGAAVASVLGQHPLMGVLAGSATLTGGPATGLAFAPFFEQAGIEGAATLAV
                     TSAMIGIVAGGLLGGPIATFLLQRRPTWIPERAADKPIAHYVEARVEEPPAGAPVGED
                     VEAYGLLKHLVLLIVAVGAGVWVSAYIEALGLTLPAYIGAMIVAAVVRNVDDRTGVIG
                     ISQRTVDDLGGIALALFLVMALMTLRLWELGAVVPLFVILTVQVALVTALCVWAVPRL
                     LGRDYDAVVMAGGFCGFMLGTTANAVANMSALVERYGPAPKAFLVVPLVGAFGIDFVN
                     GLLIAAFIDIWR"
     gene            7321..7725
                     /locus_tag="AC16CY_23215"
     CDS             7321..7725
                     /locus_tag="AC16CY_23215"
                     /inference="COORDINATES: protein motif:HMM:NF021221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HIR3356782.1"
                     /translation="MTPDPISTGLPPRLAAPLAYAGWWITGLIFWHFERRNAYVRFHA
                     AQSCVVFGAIAIVVAVLTGIAGASVVYNPPAVGFWSWTAAIAWGAGLVLWPVAMWRAA
                     TGRLWRIPVAASVADRLCRQSDEATASSAAGS"
     gene            complement(7697..8170)
                     /locus_tag="AC16CY_23220"
     CDS             complement(7697..8170)
                     /locus_tag="AC16CY_23220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356783.1"
                     /translation="MSRPRRLFLSLAGVAFLSAAVSAGQTGKPAVTPPPASTQKPADE
                     GAPKKLVAPVRGEALIGYLTPVTKPEGNMIVTTIRIKNLSSGAIAGLKVDEFWFDKAG
                     QPVTGAQTFRYRKPLQPGEVIDVVLRVPRDDRMNRNTYQFEHANGKIKTQQLKKL"
     gene            complement(8203..8667)
                     /locus_tag="AC16CY_23225"
     CDS             complement(8203..8667)
                     /locus_tag="AC16CY_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356784.1"
                     /translation="MAPRKSNVAKTKCALCEAKEVSEPRGEERYCRDCWEKKIAVEEI
                     VAREFALKRYIRAHSAEKYLIYHSTLKRPCGQILVVDDGYDLFLSVILYPNFGWDEQA
                     YHLDGDPEGRSFAEILVDVVATEVIEPWGGGKWHLEIFRSVNPEPEDWNGEM"
     gene            complement(8699..10831)
                     /gene="glgP"
                     /locus_tag="AC16CY_23230"
     CDS             complement(8699..10831)
                     /gene="glgP"
                     /locus_tag="AC16CY_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR3356785.1"
                     /translation="MTPLPERLSRMEALARDVWWSWTPTARQVFRSLDYPLWRVTAHN
                     PVRMLQQVSPEALQRALENPEWLANYDAAVARFDAARQSTTTWCDRECPDLAGKPIAY
                     FSAEFALHQSLPIYAGGLGVLAGDHCKEASDLGVPLVGVGFMYPQGYFHQNLTSDGWQ
                     QEVYEKLNWTGAPIEPAVTPEGKPCVTAVPLGNRTVLVAVWRVRVGRVALYLLDTDLE
                     ENAPWDRALSARLYGGDRETRVQQEIILGVGGVRVLKAMGYTPLVYHLNEGHAAFVVL
                     QRIRDLCGGGASFDDALAEVRRTTVFTTHTPVAAGHDAFPFHLVETHLAGAWGDLGGH
                     RERFLSLGHYDNGSGPMFNMTALALRTSGAINGVSRLHGEVTKQMWHPIWPGVPYEKL
                     PVRPITNGVHVPTWMSAEIGSLLERHLGANWLDSHDDPAVVDRVMDIPDEELWVARQS
                     LRAFLFNFIRERARARWTHEQVSTARIVAAATLFDHNTLTLGFARRFTSYKRPELIFT
                     DPDRLARLLNAPGRAVQIVFAGKAHPADEAGKHHLQQIFRRTLDPKFGGRIGFVDDYD
                     LHVAHFLVQGCDVWMNNPRKPMEASGTSGMKAAINGTPHLSIGDGWWAEGFTGENGWL
                     IEGRSDSADHGAQDWADAGALYTLIEEQLVPTFYDRDAQGVPRKWLQIVKQAIRTVLP
                     GFSARRMVKDYVREMYLPAVSQPVAGSR"
     gene            10967..11173
                     /locus_tag="AC16CY_23235"
     CDS             10967..11173
                     /locus_tag="AC16CY_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356786.1"
                     /translation="MARNARPTAAKREREKALIERRQQKAARRQDAKERKATTGPDRA
                     LGDDPDIAHIKLGPQPPADWQLEE"
     gene            complement(11259..11993)
                     /locus_tag="AC16CY_23240"
     CDS             complement(11259..11993)
                     /locus_tag="AC16CY_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HIR3356787.1"
                     /translation="MRRMRIGVAAISLAALGTLVAFAQAPQAPAGGQAPPGGGQGRGA
                     PKGPPSFFITSVGKGDGANYGGLAGADAYCAQMAKAGGLDPGAGRTWRAYLSQQASGG
                     QPAVNARDRIGAGPWHNARGGLIANNVADLHGDVQRDRNQINKLNALSEKNAQVNGVG
                     DMPNQHDILTGSDSHGRAVAGDMDTTCSNYTSNADGKGSVMLGHHDRLGGGNTSWNSA
                     HGSRGCSQGNLVQTGGAGLLYCFAAN"
     gene            12091..12963
                     /locus_tag="AC16CY_23245"
     CDS             12091..12963
                     /locus_tag="AC16CY_23245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LBP/BPI/CETP family protein"
                     /protein_id="HIR3356788.1"
                     /translation="MSCRLVSLGAPASAVVAAVVVGSSLEVSGQVGALDETVARLSNY
                     VARYYARAQAVVVEENVSVQPLLSDMTASGFARRLTFELRVEWSPDAAAEDAVKVVRR
                     LLKVNGRPPDDKHDPECLDPNDVSPEPLAFLLPDRRHAFTFTMAGQGSVDGQPALMLD
                     FRSVRPEPPIVEWDDECASIDLPGRSRGRLWADPETGEVLRFDEHLIGIVDIDVPLPQ
                     QRRGARPHLTVERADTSIRYRRVSFESPDETLLLPSSIETVTVIRNSGVPRVRISQTF
                     GNYRRFATDSRVVQ"
BASE COUNT         2052 a         4449 c         4270 g         2219 t
ORIGIN      
        1 tacctgactc gcagtccgag atccgagccg tctcccacga ctgtcgtgag cgtgtgcggc
       61 aggtgatgca ggttcaacac ggcgtcggtc acgcccgcgg cggacagcag acgcaggatg
      121 cgccgcacga gcggcacgcc ggcgagcggc agcgctgcct tcgcgcgaac gtacgacaga
      181 gggcgcagtc gggtggccag gccggccgtc aggacgagcg ctgggaccat tcgtccgcga
      241 acatcatgtc tgcgcccgtg aacccgaact gctcccgccg gcgcagaaac aacccgcgat
      301 aggaatcgag gaggtaatcg gagctggagc gtcccagcgc gcgcgccatc tgctcgatgg
      361 cccgctcgcc gccttcgatt tcgacgaacg tgccgatggg cgtctcatcg agcgcgatca
      421 tggcgtcctc ggccgcgaat tcctccctga acttctggta gcggaaccag acgtgcaggc
      481 cgagcgactc gagcacccgc gtcatcacgt cgccgtcgct gacgaccgtt tcgtgctcct
      541 cgcgggtctt gacgacgccc ggctggaccg ggcccttgaa ggtgagcagg ctccggcccg
      601 actcgctgcg gacgcgcaaa gcgcatccgc tgcggcggag cagctcgtcc ccggtgtcga
      661 acatcgcgtc ttcctggagc cggcggcatc tgaggggcgc cgctcccgca gccagaatgg
      721 ccgcgcgtgc ttctgaaggg ctcccgaaca tcaacttgat ctctttttcg atcatgttcg
      781 cgtcacgttg tgtcgtggcc gtttgggggc ttcaggacag ggaggcccag attatagaca
      841 ggtcgtcgaa aaaggaaagg ccccagaccg gctaccggcc tggggcccaa aaccacggac
      901 ccggaacgtc ttactccttc gtgacgggga cgaagatctc ctccccaccg cggatgaccc
      961 gcaggaaggc cgttccacct gatcgaacct ggtccagttc gcgctgggcc tcggcacggt
     1021 tctgtacctg ctgcccgcca acgcggacga tcagatctcc aggcgccagg agccgttctg
     1081 cggggccgcc cggctcgacg gccgtgacca ccgcgccccg gcgatcgcgc aggttcaact
     1141 ggcgggcgac ttcggccgtc acgtcctgta acttgaagcc gaatccgctg ctcgtctcgg
     1201 gggccggatc ggcatcgggc ttggcagcac gctcggtccg gttctccgcc tcgagatcca
     1261 gttcgtccac ggtgatgctg agcgagcgct cctcgcggtt gcggacgatg cggagcggca
     1321 ccgtggttcc gggcttcgtg ttcagcacca tctgtatcag ctcgtcgcga gcctgaacgg
     1381 gcttcccgcc gaacgcgatg atgacgtcgc ccggctcgag gccggccctg ctggccgcgc
     1441 tgtcggcaag cacccgccgg atcagcgcgc cgccgcgagt cttcaagccg agctcgtcca
     1501 ccgcctcgcg cggaatcgac agcacgtcca cgccgatgcg tccgcgtgtg accttgccga
     1561 cgcggagctg tggcagcagc tcgcgcaccg agttcgctgg gatggcgaac ccgatgccga
     1621 tgttcccctg ccgcctcgcg tccgtgtaaa tcgcgacgtt gacgccgacc acctcgccgc
     1681 ggatgttgag cagcggtccg ccggagttcc ccgggttgat ggccgcgtcg gtctgaatga
     1741 cgctgaccct gcgcccctcg gccacttcca gctcgctcgg ccggtttccg ctgatgacgc
     1801 cgacgctgac ggtgtgcgcc aggccgaagg ggttgccgat cgccatgacc cagtcgcccg
     1861 gctgcatctg cgacgagtca ccgaacctga cctcgggcag cgtgtgatcg ggcttctgcg
     1921 tcaattcgat caacgcgctg tcggtcaaca cgtcgcggcc gatcacacgt gcgtcgtatt
     1981 cctgatcctc atcctctccg tacaacgaga ccttgatctt ggtggcgccc tcgacgacgt
     2041 gattgttggt gagaatgagg ccggccttgt cgatgatgaa gcccgtaccg gctgaccgca
     2101 cgagctcctc acgcggccgc tggcgttcgt cgcgcgaatc cggctgcccg aaaaagcgct
     2161 cgaacaaccc gctgtcgccg ccgaagaact cgctcaagtc ctgcgtgcgc cgccgcgatt
     2221 ctgtccggat gttgacgacc gccgggctga cggtcttcgc gatgtcgcgg aacgtgctcg
     2281 acgtgagcgg gccgttgagc ggcgagctgt tcatcgccgg ggccggcgtc tgcgccgccg
     2341 attccggggc caggcccagc ctcgaggcga acaccagggc ggccgcggct gacgtcaccg
     2401 cgatgagtag cccgtaaaac caggtttgtc ttcggctcga catcgattcc tccaaacctc
     2461 tagtggagcg tatcagcgaa acgtagggcg ggccgcccgg tggttggccc gcgaaatcgg
     2521 cgtcgaaggg ctgtccgatt cgctacaggt tcagttgata cagtccgcta gatcctattg
     2581 tcggccaagt gcccccgatc ccggtagggc cccgagcctt tcgccttgcg atcccctcag
     2641 gcgagcgtga cgacctcgct ctgccgcggc cccgtgacga gggcctcgtg ctcgccacgg
     2701 tagacgttga tctcggtccg cacgccgaac gactcgaaat acaggcctgg ctcgatggtg
     2761 aaccccgtgc cgggcagcag ctgccggtcg tcgtgcgttt cgtagtcgtc catgtgcgcg
     2821 ccgttgccgt gcacgttttc accgaggctg tggccggtgc gatggacgat gcggtccgcg
     2881 tacccttcat ccgccagcac cacgcgcgcg gcgcgatcga cctcccatcc gtgcaggtcg
     2941 cggccgtctc gggccgcgcc gaccacgagt tcgaccgcgg cgtcccgcgc gagcgccacg
     3001 gcgtgaaacg ccttcgacac ctcacccggc acgcgggcgc tcgtcacggc gacccacgtg
     3061 atgtcggcga acacggcgcc cggatcgggc ttcttgcccc acaggtccag caggagtact
     3121 tcgttcgcga cgatcgcgcg gcagtccccg gcggccggca ggtagtgcgg gtcgcccgcg
     3181 tttccgccca cggcgacgac cggcggcgaa tcgctgacga gcccctcgtc ctcgaaccac
     3241 cgcaccatct gctgctgcag ctgatactcg ttcaacgacc ggccgcggcg cagcgcgtcg
     3301 gcggcttcct ggaacgcgcg atccttgatg cggtagagcg aggcggacgc gtcgcggtgc
     3361 gacgcgagct gctcgggcgt ccaccgggcc tcgaactgct gcacgaggtc gcctgacgac
     3421 aggacctcga cgccgcgcgc gcggaccatt tcaaccgttc cggcatcgac gcgcgacacg
     3481 tacgggatgg cgcacatcgg cgagtactcc atcgccacgc gcgtcatccc cgagagcaga
     3541 cgcgtcagcc cgttctccag ctgctggcgg cccgcataca ccagccgctc gccgggcagc
     3601 ccgtccaggt tgtgacgctc gatggcgtgc acgagcccac gcggctgtcc gctggccggg
     3661 atcaggtagt accaccgccg cgtggtcatg tgccgcccgt cggagagccc ggccaggcgc
     3721 gtcgcgatcg gattggaacc gtggaaatcg tagaggagcc acccgtcgag gccggcctga
     3781 cgaagccgtt cctggacggc cgtcacatcg atgggcatcc tccgattcta gcgggaatga
     3841 ccgtcgggcc tataatcgag gcggcctaca tgatcatgac aagacgactt gcgctcgcgc
     3901 tgatcgccgc cgctctcggc ggcgtgctcc cggcggctca acagcaggca cccgcgccga
     3961 cggccgtgga ggctcaggcg gaagtgccgc aagtgacctt ccgcgtggag gtcaactacg
     4021 tcgaggttga cgcgatcgtc accgacggcc agggcaaccc ggtcaccaac ctgacgaaag
     4081 acgacttcga gctcttcgag gacgggaggc gccaggacat cgcttcgttc tccctcgtca
     4141 acattccgat cgaacgcgcc gagcaggcgc tcttcgccac gacgccgatc gaagccgacg
     4201 tgcagaccaa cgagaacgtc gaggggcgga tctacctgct cgtgctcgac gacctgcaca
     4261 ccgacgcgtc ccgcaccgct cgcgtcaagg cggcggcgcg gcggttcatc gagcagaact
     4321 tcgggaccaa cgatctcgcg gccgtcgtgt tcacgtggcg gtccgccctt ggccaggact
     4381 tcaccaacaa caagcgcatg ctcatcgccg cgatcgacaa gttcaacggc gggaagctgc
     4441 ggtccgagac gctgaaccgg ctcgacacgg cctcgcccaa cccgcagact ggagagctcc
     4501 agccggggac cgacatcgat cagatggatc gcgcccatcg ggcacggcaa ctggcgcact
     4561 ccatcaagaa gctcgccgaa ttcatggcgg gagtccgcgg ccggcggaag gccatgctgc
     4621 tgatcgggga aggggtcgat tacgacatct accaggtcac cgggcagctg ggcgcgaccg
     4681 cgtcctcggt ggtcctcgat tcgcaggacg ccatcgcggc cgcgactcgc gggaacgtca
     4741 ccatctacac gatcgacccg cgcgggttga tgaatgcgga gaccgagctc atcgaggcgc
     4801 agggacccat aggcgatgcc ggcatccagt cctcgcagta ctccttccgc acgtcgcagg
     4861 acacccttcg tgcgctcgcc gggaacaccg gcgggttcgc ctcgctgaac cgcaacgact
     4921 tcgacacagc cttcgaccgc atcgtggccg agaacagcag ctattacctg ctgggcttct
     4981 actcgaccaa cgacaggcgg gacggccgtt tccgcaagct cgaggtgcgc gccaggcgcc
     5041 cgggcctgcg cgtgcgcaac cgcaacgggt acttcgaggc gcgcggccgc gctccggcgc
     5101 agctgcttcc gtcacagtcg ccgaacgcgc tgcctcccgc cgtgtccgcg gcggtcggca
     5161 gtccgattcc gatggccgga ttgccgatca aggtgttcgc ggcgccctac aaaggcacgg
     5221 cccccaacgc cgccgtggcg ctggtcttcg aaatcgacat caaaggcctg aacatcgtcg
     5281 agaaggacgg cacgttcaac gagcagctcg aggtcgtcta caacactgtg aacaacaaca
     5341 acagggtctt cccgggcgac cgccacaccg tcacgatggc gatgaagccg gagacctacg
     5401 agcgcgtcaa gacacacggc ctgcgcgtgc tgtctcaggc gcaactcccg ccggggcgct
     5461 accagatgag ggtcgcggcg ggagacaggt cggggacggc cggcagcgtc gtgtacgacc
     5521 tcgaggtgcc agacttcagc agcgcgccgc tggtgatgag cggcgtgtcc ctgacggcgg
     5581 catcggccgg ccagacgtgg acgatcaagc cgaaggaccc gctgggcgac gtccttccgg
     5641 gcccgccgac ggcgaggcgc gagttcgaca aggccgatct cgtggtgctc ttcgccgagt
     5701 tctacgaaaa tgcgccgacg gcacggcccc acatgctcga tttcaaggcg gagctgcgcg
     5761 agcagggtgg ccgagtcgtg cagcagattg cggacgagcg atcgtcaacc gagttgcagg
     5821 gctcgagcgg cggctacggc ttcaccgcac ggttccagct gcaggacgcc caacctgggc
     5881 tccacgtcat tcacgtcgaa gggcgatcgc gcgacaaccc ggattcggtg gtgagccggg
     5941 acattcagat ccgggtgcgt tgagccggca cggacgggga tgcgtctcga cctcgtccag
     6001 accgttgcgt ttgccggcgt gatgttgttc gccggctacg ggctcaagcg acagattccc
     6061 cttctggccc gctacaacat cccggccccc gtcgtcggcg gtcttcccgc cgccgcgctc
     6121 ttcacgctgc tccgggcact ggacctgtcg ggactcgagt tcgacacgac attgcaggtc
     6181 ccgctgcaaa acaccttctt cgcgtcgatc ggattcggcg cgagcgtgct cgtgctgcgc
     6241 cgcggcggac cgctcgtcct catcatgatg gttctggcgt cagtggcggc ggcgcttcag
     6301 aacgtcgtgg gcgccgccgt cgcatcggtg ctcggccagc accccttgat gggcgttctt
     6361 gccggatcgg ccaccctcac cggcggtccc gcgacgggat tggcgtttgc gccgttcttc
     6421 gagcaggccg gcatcgaggg cgcggcgacg ctcgcggtca cgtccgcgat gatcggaatc
     6481 gtggcggggg gcctgctcgg cggccccatc gccacgttcc tgctgcagcg gcggcccacg
     6541 tggattccgg agcgggccgc ggataagccc atcgcgcact atgtggaagc ccgggtcgag
     6601 gagccgccag ccggcgcgcc cgtcggagag gacgtggagg cgtacggcct tctcaagcac
     6661 ctcgtcctgc tcatcgtggc cgtcggcgcg ggcgtctggg tcagcgccta catcgaagcg
     6721 ctgggcctca cgctgccggc ctacatcggg gcgatgatcg tcgccgccgt ggttcgcaac
     6781 gtggacgatc gaaccggggt gatcggcatc tctcaacgga ccgtggacga tctgggcggg
     6841 atcgcgcttg cgctcttcct ggtgatggcg ctcatgacgc tgcgcctctg ggagctcggc
     6901 gccgtcgtcc cgctcttcgt gatcctcacc gtgcaggtcg cgctggtgac ggcgctgtgc
     6961 gtgtgggccg tgccgcggct cctcggcagg gactacgacg ccgtcgtcat ggccggcggg
     7021 ttctgcggct tcatgctcgg caccacggcg aacgcggtgg caaacatgag cgcgctcgtg
     7081 gagcgatacg gacccgcgcc aaaggcgttt ctcgtcgttc cgctcgtcgg cgcctttggc
     7141 atcgatttcg tcaacggact gctcatcgcc gccttcatcg acatctggcg gtaatggggt
     7201 cgggagtcat ttattgaagg ggctataaat ggggtcggga gtcattttat gaggaccccc
     7261 gcttccgaag tcgtgccaag agcgtcttta gaaaatgact cccgacccca ttttcaataa
     7321 atgactcccg accccatttc caccggtctg ccgccgcgcc tcgcggcgcc gctcgcctac
     7381 gccggctggt ggatcaccgg gctgatcttc tggcatttcg agcgccgcaa cgcgtacgtc
     7441 cgctttcacg ccgcgcagtc gtgcgtcgtg tttggcgcca tcgcgatcgt ggtcgcggtg
     7501 ctgaccggga tcgcgggcgc gtcggtggtc tacaatccgc cggcagtcgg attctggtcg
     7561 tggacggccg cgatcgcctg gggagcaggg ctggtgctct ggccggtcgc gatgtggcga
     7621 gcggcaaccg gccggctctg gcgtattccg gttgccgcct ctgtcgccga ccggctctgc
     7681 cgccagtccg acgaggctac agcttcttca gctgctgggt cttgatcttg ccgttggcgt
     7741 gctcgaactg gtacgtgttc cggttcatcc tgtcgtcgcg cggcacgcgc agcacgacgt
     7801 cgatgacttc gccgggctgg agcggcttcc tataacggaa tgtctgcgcg cccgtgaccg
     7861 gctggccggc cttgtcgaac cagaattcgt ccaccttcaa ccctgcgatc gcgcccgacg
     7921 agaggttctt gattctgatc gtcgtgacga tcatgtttcc ttcgggtttg gtcaccgggg
     7981 tcaggtagcc gatcagggcc tcgccgcgga cgggagccac cagcttcttc ggcgcaccct
     8041 cgtccgcagg cttctgagtg ctcgcgggcg gaggagtcac cgctggcttg cccgtttgtc
     8101 cggccgagac ggccgcggag agaaacgcaa cgccggcgag cgacaggaag aggcggcgtg
     8161 gacggctcat actcgaccct ccaacagtcc gggcattata ggttacatct ccccgttcca
     8221 gtcttcgggc tccgggttca cggaccggaa gatttccagg tgccacttgc ccccgcccca
     8281 gggctcgatg acctcggtgg cgacgacgtc aacgaggatt tcggcgaaac tccgcccttc
     8341 cgggtccccg tccaggtgat acgcctgctc gtcccacccg aagttcgggt agaggatgac
     8401 ggacaggaac agatcgtagc cgtcgtcaac gaccaggatc tggccgcagg ggcgcttgag
     8461 cgtcgagtgg taaatcaggt acttctcggc gctgtgcgcg cggatatatc gcttcagcgc
     8521 gaactcgcgc gcgacgatct cctcgacggc aatcttcttt tcccagcagt cgcggcagta
     8581 gcgctcctcg ccgcgcggtt cggacacctc tttcgcctcg cacagagcgc acttcgtctt
     8641 ggcgacgttg gattttcgtg gcgccattgc ttcgattata gctaccagcg acccccagct
     8701 accggctacc ggccaccggc tgactgaccg ccggaagata catctcccgc acgtagtcct
     8761 tcaccatgcg gcgggcgctg aagcccggca ggaccgtccg gatcgcctgc ttgacgatct
     8821 gcagccactt ccgcgggacg ccctgggcgt cgcgatcgta gaaggtcggg acgagctgct
     8881 cttcgatgag cgtgtacagg gcccccgcat ccgcccagtc ctgcgcgccg tgatcggccg
     8941 agtcgcttcg cccctcgatc agccagccgt tctcaccggt gaatccttcg gcccaccagc
     9001 cgtcgccaat cgacaggtgc ggcgtgccgt tgatggcggc cttcatgccg ctcgtgccgc
     9061 tggcttccat cggcttgcgc gggttgttca tccagacgtc gcacccctgg acgaggaagt
     9121 gggcgacgtg aaggtcgtag tcgtccacga acccgatccg gccgccgaac ttgggatcga
     9181 gcgtgcgcct gaagatctgc tgcagatggt gcttgcccgc ctcgtccgcc ggatgcgcct
     9241 tgccggcgaa cacgatctgc acggcgcgac cgggggcgtt gagcagccgg gccagtcggt
     9301 cggggtcagt gaagatcagc tccgggcgct tgtagctggt gaaccgcctt gcgaacccga
     9361 gggtcagcgt gttgtggtcg aagagcgtgg ccgcggccac gatgcgcgca gtgctcacct
     9421 gttcgtgcgt ccagcgcgcg cgcgcccgct cgcgaatgaa gttgaacagg aacgcccgca
     9481 gcgactgacg cgcaacccac agttcctcgt ccgggatgtc catgacgcga tcgacgaccg
     9541 ccggatcgtc atggctgtcg agccagttcg cgccgaggtg gcgctcgagc agcgacccga
     9601 tctccgccga catccatgtc ggcacgtgga cgccgttggt gatcggccgc accggcagct
     9661 tctcgtacgg cactccgggc cagatcgggt gccacatctg cttggtcact tcgccgtgca
     9721 gccggctgac gccgttgatg gcgccgctcg tgcgcagagc gagcgccgtc atgttgaaca
     9781 tcggaccgct gccgttgtcg tagtgtccca gcgacagaaa gcgctcgcgg tggccgccga
     9841 ggtcgcccca cgcgccggcc aggtgcgttt caaccagatg aaacgggaac gcgtcgtgcc
     9901 cggccgcgac cggcgtgtgc gtcgtgaaca ccgtcgtccg ccgcacttcg gccagcgcat
     9961 catcgaagct ggcgccccct ccgcagaggt cgcgaattcg ctgcaggacg acgaacgccg
    10021 cgtggccttc gttcaagtgg tagaccaggg gcgtgtagcc catcgccttg aggacgcgga
    10081 cgcctccgac gccgagaatg atctcctgct ggacccgcgt ctcgcgatcg cccccgtaca
    10141 ggcgcgccga cagcgcgcga tcccaaggcg cgttctcctc caggtccgtg tcgagcaggt
    10201 acagcgcgac gcggcccacc cgcacgcgcc agaccgcgac gagcacggtg cggttgccga
    10261 gaggcacggc cgtcacgcac ggcttgccct ccggcgtcac cgccggctcg atcggcgccc
    10321 ccgtccaatt gagcttctcg tagacctcct gctgccagcc gtcggaggtc aggttctggt
    10381 gaaagtagcc ctgtggatac atgaacccga cgcccacgag cggaacgccg aggtcgctcg
    10441 cttccttgca gtgatcgccg gcgagcacgc cgaggccgcc ggcgtagatc ggcagcgact
    10501 gatgaagcgc gaactcggcg gagaaatagg cgatcggctt gcccgccaga tcggggcact
    10561 cccgatcgca ccatgtggtg gtcgattgcc gggccgcgtc gaaccgggcg actgccgcat
    10621 cgtagttggc cagccactcc gggttctcga gggcgcgctg gagcgcctcg ggcgacacct
    10681 gttgaagcat gcggaccggg ttgtgcgcgg tgacgcgcca cagagggtag tcgagcgaac
    10741 ggaagacctg ccgcgccgtt ggtgtccagc tccaccacac gtcgcgggcc agcgcctcca
    10801 tccggctcag tcgttcaggg agtggagtca cgctcgatat cggccgggaa aattcgcacg
    10861 cgaaaaccgc atgctaacac gcagttcgac ccctcgctcc gatgcggctc cgcagcacac
    10921 ctctgtgtgt gccggggtgc aggtggtgta tgctgaagag cgccgcatgg ccagaaacgc
    10981 cagacctacc gccgcgaaac gcgaacgaga aaaggcgctc atcgagcggc gtcagcagaa
    11041 agccgcgcgt cgccaggacg cgaaggagcg aaaggcgacg acgggaccgg accgggcctt
    11101 gggcgatgat ccggacatcg cccacatcaa gctcggtccg caaccgccgg ccgactggca
    11161 gctcgaagag tagccgtctc cagactccgg tcttcagtct ccggtcgatt cgctgacgcg
    11221 aggaccgaag accgaagcct ggaagacccg atcgcgcctc agttagccgc gaagcagtag
    11281 agcagtcccg caccgcccgt ctgaaccagg ttcccctggc tgcagccgcg cgagccatgg
    11341 gccgagttcc acgacgtgtt gccgccgccc aaccgatcgt gatggccgag catcacgctg
    11401 cccttcccat ctgcgttgct ggtgtagttg ctgcaggtgg tatccatgtc gccagcgacg
    11461 gcgcgcccgt gcgagtccga tccggtgaga atgtcatgct ggttcggcat gtcacccacg
    11521 ccattgacct gcgcgttctt ctcgctgagc gcgttcagct tgttgatctg attgcggtcg
    11581 cgctgcacgt cgccgtgcag atcggccacg ttgttggcaa tcagcccgcc gcgcgcgttg
    11641 tgccacggac cggcgccgat ccggtcgcgg gcgttcacgg ccggctgccc gccgctggcc
    11701 tgctggctga gatacgcacg ccacgtgcgt ccggcccccg ggtcgaggcc gccagctttc
    11761 gccatctgcg cgcagtacgc gtcggcgccg gcgagtccgc cgtagttggc gccgtcaccc
    11821 ttgcccacgc tcgtgatgaa gaacgagggc gggcccttcg gggcgccccg gccctgccca
    11881 ccaccagggg gcgcctgtcc tcccgccggc gcctgaggcg cctgggcaaa ggccaccaga
    11941 gttcccaacg cggccagtga tatcgccgcc acgccaattc gcatcctgcg catcgtcgga
    12001 ttcctcctgt atgaatgaag tctagcggcg ccgtaatccg ctcttcgatc cctgcgccaa
    12061 ggcgggtaaa gttagcacag tccgaacgtg atgtcctgcc gcctcgtgtc tctcggcgcg
    12121 cctgcgtcag ccgtcgtcgc ggccgtcgtc gtcgggtcct cgctcgaggt ctcggggcag
    12181 gtcggcgccc tggacgagac cgtggcgcgg ctctcgaact acgtcgcgcg gtactacgcg
    12241 cgggcgcagg cggtagtggt cgaggagaac gtttccgtcc agccgctgct gagcgacatg
    12301 acggcctcgg ggtttgctcg acggctgacc ttcgagctcc gcgtcgagtg gagcccggac
    12361 gccgcggccg aagacgccgt gaaggtggtg cggcggctgc tgaaagtcaa cggccggccg
    12421 cccgatgata agcacgatcc ggaatgcctc gaccccaacg acgtgtcgcc cgagccgctt
    12481 gcgtttctgc ttcccgaccg tcgccacgcg ttcaccttca cgatggccgg gcaggggagc
    12541 gtcgatgggc aaccggccct catgctcgac tttcgatcgg tgcggcccga gcctcccatc
    12601 gtcgagtggg atgacgagtg cgcgagcatc gatctgcccg ggcgctcgcg cgggcggctg
    12661 tgggccgatc ccgagaccgg ggaggtcttg cggttcgacg agcacctgat cgggatcgtg
    12721 gatatcgacg tgcccctgcc acagcagcgg cgcggtgcgc ggccgcatct gacggtcgaa
    12781 cgagccgaca cgtcgatccg ctacaggcgc gtgtcgttcg agagtccgga cgagacgctc
    12841 ctgctgcctt cgagcatcga gacggtgacc gtcatccgga actccggcgt tccgcgcgtg
    12901 cgcatctcgc agaccttcgg caactaccgc cgcttcgcga cggattcgcg cgttgttcag
    12961 tgagaggcgg cgtgccagaa tcggccacgt
//
LOCUS       DBNGSH010001021         5782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3884976, whole genome shotgun sequence.
ACCESSION   DBNGSH010001021 DBNGSH010000000
VERSION     DBNGSH010001021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3884976"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..3009)
                     /locus_tag="AC16CY_23250"
     CDS             complement(280..3009)
                     /locus_tag="AC16CY_23250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356789.1"
                     /translation="MRDWELYVRTHLPLPHLTRERQSRIVQELATQFEDFYREAIARG
                     MTESDADAFARAQITDWIGLASTLADVARSHVRSPMDRWSERLDDRVRRKKGVSPMDL
                     VADLRYALRLLRKTPVFTAAAIGTLALGIGANTTIFSLVQTMLLRPLPYQDPDQVVMV
                     WEDRSAAGFPRNTPAPANYRDWRARNRSFTDMAAAAFAFANLTGDGGPELVIGRRVTA
                     NFFSVLGVQPVVGRTFTAADDAMGQRVVVISHALWQRRYGGDPGIVGRTISMSGQGSG
                     GAASDVKQEVVGVAPPSFVFLARDVDYWVPMQFSPEEAAVRGNHFLNVVARLKPGVTL
                     EGANSDIGAIARRLSEEYPETNRDFSEAVVVPIREQVLGNTRVQVIALMTAAAVIVLI
                     ACANLASLLLARASVRRGEYAVRLSLGATRGRLARQVLVEALCLSIAGGVLGLAIPLL
                     TTTFVERIVPVGLQSFAVSLVDWRLLVFAGALSIATGLLFSLGPVLQSARASTADVLQ
                     QHARGNTGGRSRAFRDGLVVLQVAATLVLLVAAGLMLRTLANLNAVERGFDADNVLTM
                     VVPLMPKYAEPSKRPAFYNGIIAGVRALPGVRGVAFGSLLPFQSTGNTRSFTIEGRPR
                     QPGDDLDVLFRIGTADYLQTLRVTLVEGRLLDARDGADAPRAVVVNETLVRRYLPGQS
                     ALGQHIRFDPSEPFFTIVGVVKDVLERGFEREAKPGVYVTQSQGPRFFPTVNLMVRVD
                     NDPLGYAPAVQRIIRAVDPDQPIRLVRPMTEVIALTVGDRRQQTTILVVFGALALVIA
                     SLGLYGLLAQTVSARSREIGIRMALGATWRSVMGIVMSRGMVLTGAGVCIGAVLAWSV
                     TRAMDSLLYGVGASDPVTFALVAGLLATVSAVACAIPAVRAARVDPMLVLRDQ"
     gene            complement(3002..3319)
                     /locus_tag="AC16CY_23255"
     CDS             complement(3002..3319)
                     /locus_tag="AC16CY_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3356790.1"
                     /translation="MKRGSTEMLILSLLEARPRHGYEIGKLIEARSGGKLTFALPSLY
                     PTLLRLESRGWIKGRWVEKAGERRRCFYRLTPEGRRVLTEQRKTWEAFIEAVRAVMGP
                     SHA"
     gene            complement(3436..5232)
                     /locus_tag="AC16CY_23260"
     CDS             complement(3436..5232)
                     /locus_tag="AC16CY_23260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3356791.1"
                     /translation="MRARAAWTTVPQLILVSFASLALAEPASALNADYWRGGWRTPLG
                     DEPHIYEFVIRGTRVTGVYCRNCSDATTIGFIDGTWDEKAGVNFTVTFANPAGRILST
                     DDQHAMLVDGRMIVSGIASIKGGKPLTLVKDPRGADPGGAPAYHLPPGTPPALPAGRP
                     GGGGGGGGGRGAGAAPYWQAGPFKTLRPADLVGTWIASFGLGMNRQLFTFLLVGDRLR
                     GVVCGRCDNPYTIGAIENVVLAGDRLYFDIVHQDWGEIDPPTFDRSIVAQVVQNEMLA
                     AILGNGIVVDPKSPPARPAGRGFTLVGPIAPESTRGNSSEGVDVWGTGTGSGTQPPPG
                     RTPIVPVKTFAVRVVGNVPYLQGASYPDDKDKLDLYVPEGRSNAPVIVSYYGNQLMGG
                     DKEEDAFIGRRFATAGFVTVVVNYRLTPDVSHPAHVQDAAASFAWVKRHIAEYGGNPD
                     RVFVIGYSAGGYLAALLSTDPRYLAAHNLSPRDIRGTVPVSAFYWVERRGVAPDRNTR
                     VWGTDPKVWVDASPAHHLQRNAPPMLILYADRDEDWRRQQNVEVAAAMKAAGLPSVDI
                     SMIGDRTHASIWTRVGNEGDPTAEQIIRFASR"
     gene            complement(5229..5678)
                     /locus_tag="AC16CY_23265"
     CDS             complement(5229..5678)
                     /locus_tag="AC16CY_23265"
                     /inference="COORDINATES: protein motif:HMM:NF039360.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HIR3356792.1"
                     /translation="MRFWLPALIAAAVIPAGAHHPFTPYYDASRPASITGVVAELREV
                     NPHVVLIVEGTGPDGRTGRWAFEGLPPNVFKRQGTDFRARLTPGTRITISGWPAKDPQ
                     ARAFSGRDITFADGSVMRFGLTPEEGDGWKCASGPCPYTYPDPRPQQ"
BASE COUNT          979 a         1953 c         1918 g          932 t
ORIGIN      
        1 atcgggggcc tcgggcacct tcgggtctct caggatggtc gatgggctct catcggcgtc
       61 accgaactat gggaaagcga catcatggtg gctgagggcg cctggtagct gaccgtgggg
      121 acacggattc ccagcgcggt cccctgcgct aattgatgga gttggcggcg cggcatccac
      181 tagttggttg acgcctcgag ctgcgcggtc agcacagtca tgtggcgcgt cggccgagga
      241 cgttctcatc cagcacgaag cgggggtgct cgcctgttgc tactggtcgc gcaggacgag
      301 catcggatcg acgcgagccg cgcggacggc cggaatcgcg cacgcgacgg cggacacggt
      361 ggcgagcaga ccggcgacca gcgcgaacgt cacaggatct gaagcgccga cgccgtagag
      421 cagcgagtcc atggctcgcg tgaccgacca tgccagcacg gcgccaatgc acacgccggc
      481 gccggtcagc accatgcctc gtgacatcac tatccccatc acgcttcgcc acgtggcgcc
      541 gagtgccatc cgaatcccga tctcgcggct ccgcgccgac acggtttgcg cgagaagtcc
      601 gtataggccg agcgacgcga tgacgagcgc cagcgcaccg aacacgacca ggatcgtcgt
      661 ctgttgacga cgatcgccga cggtgagcgc gatcacctcc gtcatcggcc ggaccaggcg
      721 gatcggctgg tcgggatcga ccgcgcgaat gatccgctgg actgccggcg cataaccgag
      781 cgggtcgttg tccacgcgca ccatcagatt cactgtgggg aagaagcgtg gcccctgcga
      841 ctgcgtcacg tagacacccg gcttcgcctc cctctcgaac ccacgctcga ggacgtcctt
      901 cacgacgccg acgatggtga aaaacggctc gcttggatcg aaccggatgt gctggccgag
      961 cgcggattgg cccggcaggt atcgtcggac gagcgtttca ttgacgacga cggcgcgagg
     1021 cgcgtcggcg ccatcgcgcg cgtcgaggag gcgcccttcg accagcgtca ctcgaagtgt
     1081 ctgcagatag tccgccgtgc caatgcggaa cagcacgtcc agatcgtctc caggctggcg
     1141 cggacgtccc tcgatagtga agcttcgagt gttgccggtg gactggaagg gaagcaatga
     1201 gccaaatgcc acgccgcgca cacctggaag tgcgcgcacg ccggcgatga tgccgttgta
     1261 aaacgccggc cgtttgcttg gctccgcgta tttcggcatc agcggcacca ccatcgtcag
     1321 cacattgtcc gcgtcgaacc cgcgctcgac ggcgttgagg ttggcgagcg ttcgcagcat
     1381 caggccggcg gcgacgagca gcaccagcgt ggccgccacc tggagcacga ccaggccgtc
     1441 gcggaacgcg cggctccggc cgccggtatt gccacgggcg tgctgctgaa ggacgtcggc
     1501 agtggacgcg cgcgccgatt gaagcactgg cccgaggctg aacaacaggc cggtcgcgat
     1561 cgacagcgcg ccggcaaaca cgaggagacg ccaatccacg agcgacacgg cgaacgactg
     1621 cagtccgact ggcacgatcc gctcgacgaa cgtcgtcgtc agcagcggaa tcgccaggcc
     1681 caggacaccg ccggcgatcg acaggcagag cgcttcgacg agcacctgcc tcgccagccg
     1741 tccccgcgtg gcgccgagcg agaggcgcac ggcgtactcg ccgcggcgca ccgaggcgcg
     1801 agccaggagg aggctggcga gattggcgca ggcgatgagg acgatgactg ccgctgccgt
     1861 catcaatgcg atcacctgga ccctggtgtt gccgagcacc tgctcgcgta tcgggacgac
     1921 gacggcttcg ctgaaatcgc gattggtctc cggatactcc tcgctgaggc gtctcgcaat
     1981 cgctccgatg tccgagttcg caccttcaag cgtcacaccg ggcttcagac gggcgaccac
     2041 gttcaggaag tggttgccgc gcaccgcagc ctcctcgggc gagaactgca tggggaccca
     2101 gtagtcgaca tcgcgggcga ggaacacgaa tgaaggcggc gccacgccga ccacctcctg
     2161 cttgacgtcg ctcgcagccc ctccgctacc ctgcccggac atcgaaatgg tgcgcccgac
     2221 aatgcccggg tcgccgccgt agcggcgctg ccagagggcg tggctgatca ccacgactcg
     2281 ctgtcccatg gcgtcgtccg ccgccgtgaa cgtgcggccg acgaccggtt gaacgcccag
     2341 caccgagaag aagtttgccg tcacgcgccg gccgatcacg agctccggcc caccgtcgcc
     2401 cgtcaggttc gcgaaggcga acgcggcagc cgccatgtcg gtgaacgacc tgtttctcgc
     2461 gcgccagtcc cggtaattgg cgggcgctgg cgtgttccgt gggaagcccg ccgcgcttct
     2521 gtcctcccag accatgacca cctggtccgg atcctggtac ggcagcggcc gcagcagcat
     2581 cgtctgtacg agcgagaaga tggtggtgtt ggcgccaatg ccgagtgcca gcgtacctat
     2641 cgcggcggcg gtgaagacgg gtgtctttcg cagcaggcgg agggcgtatc gcaggtccgc
     2701 aacaagatcc atgggtgaca cgcctttctt ccgtcgtacg cgatcgtcga ggcgctccga
     2761 ccaccgatcc atcggcgatc gaacgtgaga ccgggcgacg tctgccaggg tggatgcaag
     2821 gccgatccag tccgtgatct gcgcgcgagc gaacgcgtcg gcatcggact ccgtcatgcc
     2881 acgcgcgatc gcctcgcggt agaaatcttc gaactgcgtg gccagttcct ggacgatgcg
     2941 cgattggcgc tcacgcgtga ggtggggcag cggcagatgc gtgcggacgt agagttccca
     3001 atcacgcatg tgagggtccc atcaccgccc gaaccgcttc gatgaaggcc tcccacgtct
     3061 tgcgctgctc cgtcaggacg cggcgtcctt ccggggtgag ccggtagaag catcgtcgcc
     3121 gctcgccggc cttctccacc caacgcccct tgatccagcc gcggctctcg aggcggagca
     3181 acgtcgggta cagcgagggg agcgcgaacg tcagctttcc gcccgagcgg gcctcgatga
     3241 gcttgccgat ttcgtacccg tgacggggac gcgcctcgag cagcgagagg atcagcattt
     3301 cggtgctgcc tcttttcatg tccgcgctga acatatactc aggctaacga tatgtagtgt
     3361 gccttattat acggccggcc gcaagtgcgg ccgcaaggca ggctcgaggg cacacgcgtg
     3421 agttggcgaa cgcgcttatc gcgacgcgaa acggatgatc tgttcggccg tcggatcgcc
     3481 ctcgttgccg acgcgcgtcc agatcgacgc gtgcgtacgg tcaccgatca tcgagatgtc
     3541 gacgctgggg agaccggcgg ccttcatcgc cgcggccacc tcgacgttct gctgacgccg
     3601 ccagtcttcg tcccggtcgg cgtacaggat cagcatgggc ggcgcattgc gctgcagatg
     3661 atgcgccggg gaggcgtcaa cccagacctt cggatccgtg ccccacacac gcgtgttgcg
     3721 atccggtgcc acgccccgcc gctcgaccca gtagaacgcg ctgacgggaa ccgtgccccg
     3781 gatatcccgc ggtgacaggt tgtgcgcggc gagataccgc ggatcggtcg acaacaaggc
     3841 cgcaagatag ccgcccgccg agtagccgat gacgaacacc cggtcgggat tgccgccgta
     3901 ctccgcgatg tgacgcttca cccaggcgaa cgacgctgcc gcgtcctgaa cgtgggccgg
     3961 atgagacacg tccggcgtga ggcgatagtt gacgacaacc gtcacgaagc cggcggtcgc
     4021 gaaacgccgg ccgatgaacg cgtcctcctc cttgtctccg cccatcaact ggttgccgta
     4081 gtaagagacg atcaccgggg cattggagcg tccctcgggc acgtagaggt cgagcttgtc
     4141 cttgtcatcc ggatagctcg cgccctgcag gtagggcacg tttccgacga cgcgaaccgc
     4201 aaaggtcttc accggcacga tcggcgttct gccgggcggt ggttgcgtgc cggagccggt
     4261 gcccgttccc cagacgtcga caccctcgga gctgttccct ctcgtgctct ctggcgcgat
     4321 cggcccgacg agcgtgaacc cgcgtcccgc cggccgcgca gggggactct tgggatcgac
     4381 gacgatgccg ttgccgagaa tggcggcgag catctcgttc tgcacgacct gggccacgat
     4441 gcttcgatcg aaggtcggcg ggtcgatctc gccccagtcc tggtggacga tgtcgaagta
     4501 cagcctgtcg ccggcgagga cgacgttctc gatcgccccg atcgtgtagg ggttgtcgca
     4561 gcgcccgcac accacgcccc gcagccggtc accgaccaga agaaaagtga agagctgcct
     4621 gttcataccc aggccgaacg aggcgatcca cgtgccgacg aggtccgcag gccgcagcgt
     4681 cttgaacggt ccggcctgcc agtagggtgc cgcgccggcg ccacgacctc cgccgccacc
     4741 accgcctcct ccgggtcgcc ccgcgggcag cgcgggtgga gtgcccggcg ggaggtgata
     4801 tgccggtgcc ccgcctggat cggcgccgcg cggatccttc acgagcgtca gcggctttcc
     4861 gcctttgatg ctcgcaattc ccgacacgat catccgcccg tcaacgagca tggcgtgctg
     4921 gtcgtcggta gacaggatgc ggccggccgg attcgcaaac gtgaccgtga agttgactcc
     4981 ggccttctcg tcccacgtgc cgtcgatgaa gccgatcgtg gtggcgtcgg agcagttccg
     5041 gcaatacacg ccagtgaccc gggtgccccg gatgacgaac tcgtagatgt gcggctcatc
     5101 cccgagcggc gttcgccacc cgccacgcca gtagtcggcg ttgagcgccg acgccggctc
     5161 tgcgagcgcc aggcttgcga acgacaccag gatcagttgc ggcacggtcg tccatgccgc
     5221 tcgggctctc attgctgcgg gcgcgggtcg gggtacgtgt acgggcaagg gcccgaggca
     5281 catttccaac cgtctccctc ttccggcgtc aatccgaagc gcatcaccga cccgtccgcg
     5341 aacgtgatgt cgcgtccgct gaatgcgcgc gcctgcggat ccttcgccgg ccatccagaa
     5401 atcgtgatcc tcgttccggg cgtcagccgt gccctgaagt cggtgccctg tctcttgaac
     5461 acgttgggcg gcaatccttc gaacgcccag cggccggtgc ggccgtccgg tcccgtccct
     5521 tcgacgatca gcaccacgtg cgggttgacc tcgcggagct cggcgaccac gcccgtgatc
     5581 gacgccggcc tggacgcgtc gtagtacggc gtgaacgggt ggtgcgcccc ggcggggatc
     5641 acggcagccg cgatcagcgc cggcagccag aatctcatgt gtttcattgc ggtaaggcga
     5701 acgcgatgta cgcgcccggc agcgttggcc cgccgccgcc acggccgcct cttccggccg
     5761 ccgctccggc ctggccgccc tg
//
LOCUS       DBNGSH010001022         3602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_150457, whole genome shotgun sequence.
ACCESSION   DBNGSH010001022 DBNGSH010000000
VERSION     DBNGSH010001022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_150457"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC16CY_23270"
     CDS             complement(<1..308)
                     /locus_tag="AC16CY_23270"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011935411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR3356793.1"
                     /translation="MIPEPALTAVIALLGLCIGSFLNVCIFRLPRGESLAWPGSRCTS
                     CGRTLSWFENVPIVSWVVLRGRCRSCRTPISWIYPLVELLTAVTLVGVYELYGLTAFG
                     G"
     gene            complement(305..1195)
                     /locus_tag="AC16CY_23275"
     CDS             complement(305..1195)
                     /locus_tag="AC16CY_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356794.1"
                     /translation="MHGPLISPWRWIALALLLPAAVGAQVAFEDVYPPRDTRPETLWV
                     RSPGLMRRLSVGFAAVMADVYWIRAVQYYGDTKLSMTTNRNYNLLYPLLDTTTTLDPR
                     FNIAYRFGAILLSEGYPNGPGEIDGAIALLQKGIRAMPEKWQYYHDAGFVEYWWRDHK
                     AASEWFLKASKVSGAPPFLPTIAARALADGGDRATARALWQGLATAEHEWLRQSARRA
                     LMRLDAEDEIEALQRIVDAFHGDTGRYPAGWIELAQLGRIRGVPVDPSGVPYALDPVS
                     GVVGVTPDSSLYPLRPVRKP"
     gene            complement(1205..2011)
                     /locus_tag="AC16CY_23280"
     CDS             complement(1205..2011)
                     /locus_tag="AC16CY_23280"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356795.1"
                     /translation="MRAIAVIAGGVFRDSIRDRIAYGLVFFAVLLIASSFLLARLTAG
                     QDVKIIKDLGLAASSGIGVLIAVFFGIGLVTKEVERRSIYSLLSKPVSRTEFVVGKYL
                     GLVLTLLINLSVMVAAFYAVLAVLNYLAIPDLRASWDAPALDPRMLVAFALIGVELML
                     VTATALFFSTFSSPFLSVALTFAIYIIGHFGEDLKHLDNIVESQTLAQVARGVYYVMP
                     NLAALDVKSEVVHGVAVSAQHVLAASAAAGLYIALILVAATVIFSHRDLK"
     gene            complement(2008..2970)
                     /locus_tag="AC16CY_23285"
     CDS             complement(2008..2970)
                     /locus_tag="AC16CY_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3356796.1"
                     /translation="MDAISIEGLTKHYRVGFWRPRPYVALDGLTLGVQPGEIFGFLGP
                     NGAGKTTTLKLLMQLIYPTAGRAEILGAHVGDASVKRRIGYLPENPYFYDNLTAEELL
                     EYFARLFGYSAAEGRRRASSLLDEVGIGPERRFALRRFSKGMVQRVGIAQALINDPEI
                     VFLDEPMSGLDPLGRREVRELILRLRDKGRTVFFSSHVLSDAEALCSRVAIVVNGRLV
                     ATGSLDELTAFEAKGWELVVNGLPGAVLDGLRSSVSGATRLGDGRYALTLPPSAAPEP
                     VIAALSAAGARVVSLTPLRATLEDVFVEQVRAASGDRSKATLAS"
     gene            complement(3016..>3602)
                     /locus_tag="AC16CY_23290"
     CDS             complement(3016..>3602)
                     /locus_tag="AC16CY_23290"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3356797.1"
                     /translation="QIAEWEQWLAAQTPSTVADVLPSGDTKILVLKFNDFQCPSCRQA
                     WLLYKDIVAKYESQYPGAFRFENRDFPLETECGAGNAGHIAACEAAAAVRMAREKNKE
                     RELEAALFNRQSPTMSRDDVKAALHEIAQIDDFDARYAKVLETVRLDVQLGQKLGVKG
                     TPTFFLNGIQLPSLRPAYFDAALAWAARQSGVNP"
BASE COUNT          659 a         1191 c         1202 g          550 t
ORIGIN      
        1 ccgccgaacg ccgtcagtcc gtacagctcg tagacgccga ccagcgtcac agcggtcagc
       61 agctccacca ggggatagat ccacgagatc ggcgtccggc agctccggca gcggcccctg
      121 aggacaaccc agctgacaat cgggacgttc tcgaaccacg acagcgtgcg gccgcacgac
      181 gtgcaccgtg agcccggcca ggcgagcgac tcgccccgcg gcaggcggaa gatgcacacg
      241 ttgaggaagc tgccgatgca cagcccgagc agcgcgatga ccgcggtcaa ggcgggctcg
      301 ggaatcacgg cttccggacc ggccgcagcg gatagagcga cgaatcggga gtcacgccga
      361 caactcccga gacgggatcg agagcgtacg ggacacctga tggatcgact ggtactcccc
      421 tgatccggcc cagctgcgca agctcgatcc atccggccgg gtaccggcct gtgtcgccgt
      481 gaaaggcgtc gacgatccgt tgaagcgcct cgatctcgtc ctctgcgtcc aggcgcatga
      541 gcgcgcggcg ggcagactgg cgcagccatt cgtgctccgc cgtggcaagc ccctgccaca
      601 gcgcgcgcgc cgtggcgcga tcgcctccat cggcgagcgc ccgcgcggcg atcgtcggca
      661 ggaagggcgg cgcgccggac accttcgacg ccttgaggaa ccactccgac gccgccttgt
      721 ggtcccgcca ccagtactcc acgaagcccg cgtcgtggta gtactgccac ttctccggca
      781 tggcccggat ccccttctga agcagcgcga tcgccccatc gatctcgccc ggtccgttcg
      841 gatatccctc cgacagcagg atcgcgccga agcgatacgc gatgttgaat cgaggatcga
      901 gcgtggtcgt cgtatcgagc aatggataga gcaggttgta gttcctgttc gtcgtcatgg
      961 acagcttcgt gtcgccgtag tactgaacgg cacgaatcca gtacacgtcg gccatgacgg
     1021 cggcgaagcc gaccgacagg cgccgcatca gccccggaga gcggacccac agagtctccg
     1081 gcctcgtgtc acgggggggg tagacgtcct cgaaagccac ctgggcgccg actgcggcag
     1141 gcagcagcag cgccaacgca atccatcgcc agggcgagat gagcggtccg tgcatgtgtt
     1201 cgccctactt gaggtcgcgg tgcgagaaga tcaccgtcgc cgcgacgagg atcagagcga
     1261 tgtacagacc ggccgccgcg ctcgccgcaa gcacgtgctg cgcgctgacg gccacgccgt
     1321 gaaccacttc cgacttgacg tcgagcgcgg ccaggttcgg catcacgtag tagacgcctc
     1381 gcgcgacctg cgcgagcgtc tgtgactcca cgatgttgtc gaggtgtttc aggtcctcgc
     1441 caaagtgccc gatgatgtag atggcgaagg tcagggcgac cgacaggaac ggactcgaaa
     1501 acgtcgagaa gaagagcgcc gtcgcggtga cgagcatcag ctcgactccg atgagcgcga
     1561 acgccacgag catgcgcgga tccagcgccg gcgcatccca cgacgcccgg agatccggta
     1621 tggcaaggta gttcaagaca gcgagcaccg cgtagaaggc ggcaaccatg accgacaggt
     1681 tgatgagcag cgtcagcacg agtccgaggt acttgccgac gacgaactcg gtgcggctga
     1741 ccggcttcga gagcagggaa tagatcgagc gccgctccac ttccttggtg accaggccga
     1801 tgccgaagaa cacggcgatg agcacgccga tccccgagga cgcggccagt cccaggtcct
     1861 tgatgatctt gacgtcttgt ccggcggtga gcctggccag caggaacgac gaggcgatca
     1921 acagcacggc gaagaacacc agtccgtacg cgatgcgatc gcgaatcgag tcgcggaaca
     1981 ccccgccggc gatgaccgca atcgctctca tgacgccagc gtcgctttcg atcgatcgcc
     2041 ggaggccgcc cgcacctgtt ccacgaagac gtcctcgagc gtggcgcgca gcggggtgag
     2101 cgacacgacg cgggcacccg ccgcggacag ggcggcgatg acgggctccg gtgcggccga
     2161 cggcggcagc gtgagcgcgt agcggccgtc gcccagccgc gtggccccgg agacggaaga
     2221 gcgcaggccg tccagaaccg cgcccggaag gccgttgacg accagttccc accccttggc
     2281 ttcgaatgcg gtgagctcgt cgagcgatcc ggtcgcgacg aggcgaccgt tcacgacgat
     2341 cgccacgcgg ctgcacagcg cctccgcgtc actcaacacg tgcgaactga agaagacggt
     2401 ccggcccttg tctcgaagcc gcaggatcag ctcgcggacc tcgcggcggc cgagcgggtc
     2461 gaggcccgac atcggctcgt cgaggaacac gatttccgga tcgttgatga gcgcctgggc
     2521 gatgccaacg cgctgcacca tgccctttga gaaacggcgg agcgcgaacc ggcgttccgg
     2581 cccgatgccg acctcgtcga ggaggctcga ggcacgccgg cgtccttccg ccgccgagta
     2641 cccgaacaga cgggcaaagt actcgagcaa ctcctcagcg gtcaggttgt cgtagaagta
     2701 cggattctcg ggcaggtacc cgattcgccg tttgaccgag gcgtccccga cgtgggcgcc
     2761 gagaatctcg gctcggccgg ccgtcgggta gatcagctgc atgagcagct tgagcgtggt
     2821 ggtctttccg gcgccattcg gcccgaggaa gccgaagatt tcgccaggct ggacgccgag
     2881 cgtcaggccg tcgagggcga cgtagggtcg cggccgccag aagccgaccc ggtaatgctt
     2941 cgtcagccct tcgatgctga tggcgtccat accatcagtg tttttcggtc cgtcggctgc
     3001 cggacctgag tcccgtcagg ggttgactcc gctctgccga gccgcccagg ccagcgcggc
     3061 gtcgaagtat gccggtctga gggacgggag ctggatgccg ttcagaaaga aggtcggcgt
     3121 gcccttcacg cccagcttct gcccgagctg cacgtcgagg cgcacggttt cgaggacctt
     3181 ggcgtaccgc gcgtcgaaat cgtcaatctg tgcaatttcg tggagggccg ccttcacgtc
     3241 gtcgcgcgac atggttggcg actggcggtt gaaaagcgcg gcctcgagct cccgctcctt
     3301 gttcttctcg cgcgccatgc gaacggccgc ggccgcctcg caggcagcga tatggcccgc
     3361 gttgcccgct ccgcactccg tctcgagcgg gaagtcgcgg ttctcgaaac ggaaagcgcc
     3421 agggtactgg gattcgtact tcgccacgat gtccttgtag agcagccagg cctggcggca
     3481 ggacggacac tggaagtcgt tgaacttgag caccaggatc ttcgtgtcgc ccgacggcag
     3541 gacgtcggcc accgtcgagg gcgtttgtgc cgcgagccac tgttcccact cggcgatctg
     3601 ct
//
LOCUS       DBNGSH010001023         6535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1169424, whole genome shotgun sequence.
ACCESSION   DBNGSH010001023 DBNGSH010000000
VERSION     DBNGSH010001023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1169424"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="AC16CY_23295"
     CDS             <1..452
                     /locus_tag="AC16CY_23295"
                     /inference="COORDINATES: protein motif:HMM:NF052145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
                     /protein_id="HIR3356798.1"
                     /translation="PIVVPPLRERLDDIPGLVWEFVNEFSKSLGKSISSISKDSMRQL
                     QLYPWPGNIRELRNVIERAVIMATGPRLTVPMPQPGVARATQTQTALTLKELEIEHIR
                     TALKTTNWRVRGRGGAAERLGLKPTTLESRMAKLGVMRPKDIVGAGL"
     gene            complement(449..2098)
                     /locus_tag="AC16CY_23300"
     CDS             complement(449..2098)
                     /locus_tag="AC16CY_23300"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3356799.1"
                     /translation="MPTPRPRFWFASVVLALAVPGSMASAQGGANAPAAAPAAQAEPQ
                     KDLLGRDTPRGTVLGFMSAARDGKNDVAPLYLNTRLRGQAAVDLARQLYVVLDSRLPV
                     RVNELSDRREGSLANPLRPDQDVVGTVSTATGQLDLVVERVTVEGQSVWLFARATLER
                     IPDVHDEIDLVPIDRYLPGFLAVRIVGIRLFEWLLLIVVLPIGYRLLGLLGWAVSKSV
                     TPYLQRRFHVWDGPITHHFPGALRLIVLGIVIRTASSSFELPLLERQFWAVTAFFLAI
                     CGLVWSATLLNAFGERYIYRHLNASRLGESVALLRLGRRVADVLVYAAAFLLVLWRFD
                     RDVTAVLAGLGIGGIAVALAAQKTLENFIGGVSLVFDKAVRVGDFLRLGTTEGTVDEV
                     GLRSTRIRTLERTIVTVPNGVIASASIETVSDRDKFWFHHFVGLQYDTTPEQMRAVTD
                     NMRDLLARQPGVELESVRVRFLRLGAFSLDIELYAYVFAHDWSAFLEIQEKLLLRVME
                     IIERAGTAIAFPSQTLHMAEGDRPPELEKGAKSKADPPGPV"
     gene            2353..2916
                     /locus_tag="AC16CY_23305"
     CDS             2353..2916
                     /locus_tag="AC16CY_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HIR3356800.1"
                     /translation="MRKLSVFFVALLLAGASAAFAQDVRYNFDKQANFSSFKTYRWVQ
                     IKGAQAPNELVDGQIKAAIDSELHTKGLSRVETDSADLYIGYQVAVNTEKEYTTFNTG
                     WGTGPGWYGGGWYGGGGMSTTTGTTSTIYVGQLAVDMYSSAGKALIWRGNASKTIDTN
                     AKPEKQQKNLAKAVKKLFKNYPPPVKK"
     gene            3033..3380
                     /locus_tag="AC16CY_23310"
     CDS             3033..3380
                     /locus_tag="AC16CY_23310"
                     /inference="COORDINATES: protein motif:HMM:NF023390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIAA family protein"
                     /protein_id="HIR3356801.1"
                     /translation="MHHERDNVYRFDIRGTLTKAEMDQASSAVVVEMKRIGKVRLLFV
                     LIGFKGWEKNQAWNDLTFYVQHGDQIERIAIVGDEQWKSEAMMFAAADLRSAPVEFFP
                     SRAMAEARAWLSS"
     gene            3430..5259
                     /locus_tag="AC16CY_23315"
     CDS             3430..5259
                     /locus_tag="AC16CY_23315"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3356802.1"
                     /translation="MSHSLATRLIVWSLTVTGAVYLLTIGLSNRAGRQTAIAAAQREA
                     YAGADAAARAIDDVLHAVEESGAALGRAVSELEPDRDALDRLVERFSSDARGTSARYQ
                     VVRDPSDEAPSWYREARERGSPAWSEPYRDASGEGGTIITWSLPVPKAGGFGAVVAAS
                     VRLDFLSSVLRDAQPGTNGFALVLSRQGLLIAHTRRDLSKAVHDPLAELSPELRSIVA
                     PIVDRARAGESGFGTLSIDGEPYRITFRPISRTGWSLATAYAERDVLADVASLRRTQI
                     ALAVSGLLILGMAVVMLSRRITRPLVGLAASAGRLATGDLDGPLPEAASRDEIGALTT
                     AFRHMRDSLKDYIRNLQETTAAKERLEGEMKAARRIQADMLPAPEAGSGPEGYQLSAA
                     LVPALAVGGDLFHHFEHQRRVFFLVGDVSGKGVAAALFMARTKTLFDAVAATQRDPGG
                     VLAALNDSLCRQNQSGMYVTVVCGALNLDTGVVSFATAGHEPPVIVGPKRSPHPLETE
                     GGPILGLLPIASFPVSSVALEEGDALVMYTDGVSEARDAAGGFFGPERLLEAAARSAS
                     GDATAITTGLLQAVKAFAADAPQSDDITILTLRLTRRPILEVT"
     gene            complement(5534..6052)
                     /locus_tag="AC16CY_23320"
     CDS             complement(5534..6052)
                     /locus_tag="AC16CY_23320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356803.1"
                     /translation="MRVNDSIRRQMIACAVCALCVTASACDESTEVNPLPTSSMITAS
                     ITEVPSQLPVEQPAFVNFGCLATSLTYAPRPLSRRFDIVITALTPVDVDSVTIQMLDG
                     TSLGGPMLTFPRAKLTERFGDLRVAAGIPRRFGFDSQFPCGTSATSVLADIGVRYPRG
                     GMQVLTASQALR"
     gene            complement(6311..>6535)
                     /locus_tag="AC16CY_23325"
     CDS             complement(6311..>6535)
                     /locus_tag="AC16CY_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356804.1"
                     /translation="HDGLYSIDLRPLLNGRANDHRFVGTNPGVVVSWSATPHLTLIGV
                     ISRFFSSDFLENTFVDRGFGFYSSSLTYRF"
BASE COUNT         1186 a         2141 c         2162 g         1046 t
ORIGIN      
        1 tccccatcgt cgtaccgccg ctccgcgagc gcctcgacga catcccgggg ctcgtgtggg
       61 agttcgtcaa cgagttctcg aagtcgctcg gcaagtcgat ctcgtcgatc tcgaaggact
      121 cgatgcggca gctgcagctc tacccgtggc cgggcaacat ccgcgagctg cgcaacgtca
      181 tcgagcgggc cgttatcatg gcgaccggcc cccggctgac cgtgccgatg ccgcagcccg
      241 gcgtcgcccg cgcgacgcag acgcagacgg cgctgacgct caaggagctt gagatcgaac
      301 atatccggac tgcgctcaag accacgaact ggcgggtccg cggcaggggg ggcgcggccg
      361 aacggctggg cctgaagccg acaacgctcg aatcgcgaat ggcgaagctg ggcgtgatgc
      421 gaccgaaaga catcgtaggg gccggtcttt agaccggccc aggcgggtct gccttcgact
      481 tcgcgccttt ttcgagctcg ggggggcggt ccccttcggc catgtgcagc gtctgtgacg
      541 gaaacgcgat cgcggttccc gcgcgctcga tgatctccat gacccgcagc agcagcttct
      601 cctggatctc caggaaggcc gaccagtcgt gggcgaagac gtaggcatac aactcgatgt
      661 cgagcgaaaa ggcgccaaga cggaggaacc tgacgcgcac gctctcgagc tcgacgccgg
      721 gctgccgggc gaggagatcg cgcatgttgt ccgtgaccgc gcgcatctgc tccggcgtcg
      781 tgtcgtactg gagcccgacg aagtggtgaa accagaactt gtcgcggtcc gacacggtct
      841 cgatgctcgc ggacgcgatc acgccgtttg gaacggtcac gatcgtccgc tcgagcgttc
      901 ggatgcgcgt cgagcggagg ccgacctcgt caaccgttcc ctcggtcgtg ccgagacgca
      961 ggaagtctcc gactcgtacg gctttgtcga atacgagcga gacaccgccg atgaagttct
     1021 ccagcgtctt ctgcgccgcg agcgccacgg cgatgccgcc gatgccgagc ccggcgagca
     1081 ccgccgtgac atcgcggtcg aaccgccaca agacgagcag gaaggcggcc gcgtacacga
     1141 gcacgtccgc gacacgacgt ccgagccgca gcaaggcgac gctctcaccg agcctggagg
     1201 cgttgaggtg ccggtagatg tagcgctcgc cgaaggcgtt cagcagggtc gccgaccaca
     1261 ccaggccgca gatcgccagg aagaaggccg tcaccgccca gaactgtcgc tccaggagcg
     1321 ggagctcgaa actgctcgac gcggtccgaa tcacgatgcc gagcacgatc aggcgcaggg
     1381 cgccgggaaa gtggtgcgtg atcggcccat cccagacgtg aaagcgacgc tgcaggtacg
     1441 gcgtcaccga cttgctgact gcccagccaa gcaggccgag cagccgatag ccgatcggaa
     1501 ggacgacgat cagcagcagc cactcgaaca ggcggatgcc gacaatgcgg accgccagga
     1561 acccggggag gtagcgatcg atcgggacga gatcgatttc gtcgtgcacg tcgggaatgc
     1621 gctcgagtgt cgcgcgagcg aacagccaca cgctctgacc ttcgaccgtg acgcgctcga
     1681 cgacgagatc gagctgaccg gtggcggtgc tgacggttcc cacgacgtcc tgatccggcc
     1741 tcagtgggtt ggccagcgag ccctcgcgcc gatcgctcag ctcgttcacg cggacgggca
     1801 gccggctgtc gaggacgacg tacagctggc gcgcgaggtc gacggccgcc tggcccctca
     1861 agcgtgtgtt caagtacagc ggagccacgt cgttcttccc gtcccgggcg gcgctcatga
     1921 atccaagtac ggtcccgcgc ggcgtgtcgc ggccaagcag gtccttttgc ggctcggcct
     1981 gggcggcggg cgcggctgcg ggagcattcg caccaccctg ggccgaggcc atcgagccgg
     2041 gcacggcgag ggcgagaacg acggacgcga accagaaccg gggcctgggt gttggcatgt
     2101 gtcacctgag gccgtccatt ctcctcaatt gccacccgct ctgcgaggcg catgccggcc
     2161 cctgaagcgt gagcggatcc tcctgcccgt tggaggcggt ccgaaatctc ggatcggcag
     2221 ccccgcgggc tggacactgg taacgccgaa tacaatccca gaggctaaaa taggaggccg
     2281 cgctcgggtc gaggtacggc tcgtcgtttg caccgcagca ggcgcgttcg accttggagg
     2341 aaaggtacgc acatgagaaa gctgtccgtc ttcttcgtgg cgctgctcct ggccggcgca
     2401 agcgccgcat tcgcccagga cgttcgatac aacttcgaca agcaggccaa cttctcgtcg
     2461 ttcaagacct acaggtgggt ccagatcaag ggcgcacagg cgcccaacga actggtggac
     2521 gggcaaatca aggccgcgat cgattccgag ctccacacga aggggctctc acgggtcgag
     2581 accgactccg ccgatctcta catcggatac caggtcgcgg tgaacaccga gaaggagtac
     2641 accaccttca acaccggctg gggcacgggc ccgggttggt acggcggagg ctggtacggc
     2701 ggcggtggaa tgagcaccac caccggcacg acgtcgacga tctacgtcgg tcaactggcg
     2761 gtggacatgt actcgtctgc gggcaaagcg ctcatctggc gcggaaatgc cagcaagacg
     2821 atcgacacga acgcgaagcc ggaaaagcag cagaagaacc tggccaaggc cgtcaagaag
     2881 ctgttcaaga actatccgcc gccggtgaag aagtaggatc acggtacacg gggagcccgg
     2941 tcgccccggg ccccccggac cgtctccgct gcccgctgcc ttactgaggc ttgggccctg
     3001 agccctggcc cctgctactt atgtccgtct caatgcacca cgaacgagac aacgtgtacc
     3061 gcttcgacat tcgcggcacg ctgaccaagg ccgagatgga tcaggcgtcg tctgcggttg
     3121 tcgtcgaaat gaagcgcata ggcaaggtca gactgctgtt cgtcctgatc gggttcaagg
     3181 gatgggagaa gaaccaggcc tggaacgacc tgacgttcta cgtgcagcac ggcgaccaga
     3241 tcgagcggat cgctatcgtg ggcgacgagc agtggaagag cgaggcgatg atgttcgcgg
     3301 ccgccgacct gcggagcgct cccgtggagt tcttcccgtc acgggccatg gcagaggcac
     3361 gcgcttggct ttcatcctga tctagggttc actggcttcg gtgcgttcgt ctcgggcgct
     3421 ggcacgcgca tgtctcacag cctcgcaacg cggctcatcg tctggagtct tacggtcacg
     3481 ggcgccgtgt atctcctgac gatcggcctc tcgaaccgtg ccggacgaca gaccgcaatc
     3541 gccgcggcgc agcgcgaagc ctacgccggc gccgacgcgg ccgcgcgcgc gatcgacgat
     3601 gtgctgcacg cggtcgagga gagcggcgcg gcgctcgggc gtgcggtctc tgaactcgag
     3661 cccgaccgcg acgccctcga ccgactcgtc gaacgtttct cgtccgacgc tcgcggcacg
     3721 tccgcaaggt atcaggtcgt cagggaccca tccgacgagg ctccctcgtg gtaccgcgag
     3781 gcgcgcgagc ggggctcacc agcctggagc gagccatacc gggatgcgag cggtgaaggc
     3841 ggcaccatca tcacgtggtc gcttcccgtg ccgaaggccg gcggcttcgg cgccgtcgtc
     3901 gccgccagcg ttcgactgga cttcctgtcg tcggtgctcc gcgacgctca accggggacg
     3961 aacgggttcg cactggtgct gtccaggcaa ggcctgctca tcgcgcacac gcgtcgagat
     4021 ctcagcaagg ccgtccacga tccgctggcc gagctgtcgc ccgagctccg gtccatcgtc
     4081 gcgccgatcg tggatcgtgc ccgggccggt gagtccgggt tcggaaccct ctcgatcgac
     4141 ggcgagccct accggatcac gttccgtccg atcagccgca ccggctggtc gctggccact
     4201 gcctatgcgg aacgcgatgt cctcgcggac gtggcctcgc tgcgccggac gcagatcgcc
     4261 ctcgccgtgt cagggctgct gatcctcgga atggccgtcg tgatgctttc ccgccgtatc
     4321 acgcgtccgc tcgtcggcct ggcggcgagc gcaggccgcc tggccacggg cgatctggac
     4381 ggtccgctgc cggaagcggc ctcgcgcgac gagatcggcg cgttgacgac ggcgtttcgc
     4441 cacatgcggg attcgctgaa ggactacatt cgcaacctgc aggagacgac cgcggccaag
     4501 gaacggctcg aaggggagat gaaggcggcc cgccgcatcc aggccgacat gctgccggcg
     4561 ccggaggcgg gaagcgggcc cgagggctat cagctgtcgg cggctctcgt cccggccctg
     4621 gccgtcggcg gcgacctgtt ccatcacttc gaacaccagc gccgtgtctt cttcctggtc
     4681 ggcgacgtgt cgggcaaggg ggtggcggcc gcactcttca tggcgcgcac caagacgctg
     4741 ttcgatgcgg tggctgcaac ccagcgcgat cccggtggtg tgctggcggc gctcaacgac
     4801 agcctgtgcc ggcagaacca gtcgggcatg tacgtcaccg tcgtgtgcgg cgctctgaat
     4861 ctcgataccg gcgtcgtcag cttcgcaacc gccgggcacg aaccgcctgt gatcgtgggc
     4921 cccaagcgga gccctcatcc gctcgagacc gaggggggac cgatccttgg actactgccc
     4981 atcgccagct tccccgtctc gagcgtcgcg ctcgaggagg gggacgcgct ggtgatgtac
     5041 acagacggcg tctccgaggc tcgagatgcc gccggcggct tcttcggccc cgagcggctg
     5101 ctcgaggcag ccgcgcgcag cgcgagcggg gacgccactg ccatcaccac gggccttttg
     5161 caggcggtca aggccttcgc ggcggacgcc cctcaatccg acgacatcac gatcctgacg
     5221 ctcaggctga cgcgacgacc gattctagag gtcacttgaa tcgagcacga gatcggtcgt
     5281 tcacccttct ccgttctcgc ttctccgttc gtgttcaggt cccggtacta cggccgacat
     5341 cgccaagaac ccccgcggga acgccgaacc ctcgcacacg acgaaccctc gaaccgaacg
     5401 tgcccgcctt cgcgcgaagc gcttcggcga ggtctcgccg tagccttggc ggaggcggaa
     5461 acacgaaccg agaaggccag aagcgagaag cgtgaacaca cgatctcttg ctcaactact
     5521 agtacttcga cgttcagcgc agcgcctgcg acgccgtgag gacctgcatg cctccacggg
     5581 gataccgcac gccgatgtcg gccagcaccg aggtcgcgct cgttccgcag gggaactgcg
     5641 aatcgaaccc gaagcgcctg ggtatgcctg ccgcaacgcg cagatcgccg aagcgctccg
     5701 tcagtttcgc tcgagggaac gtcagcatcg ggccgcccag gctcgttccg tcgagcatct
     5761 gaatcgtgac gctgtccaca tcgaccggcg tcaacgccgt gatgacgatg tcgaagcgcc
     5821 gactgagggg ccgcggtgcg tacgtgaggc tcgtggcgag gcagccgaag ttgacgaacg
     5881 ccggttgttc gaccggcagc tgggacggca cctcggtgat ggacgctgtg atcatcgacg
     5941 atgtcggcaa cgggttgacc tccgtgcttt catcgcacgc ggacgcggtg acgcacaagg
     6001 cgcacacggc gcacgcgatc atctgacgac gaatcgagtc gttgactcgc atgattcctc
     6061 cttcgacccg tgtctcttac accacagctc gcttgtgttg aggctccacc gcccgatcgc
     6121 aaccagaaag gactgccgat tccgccgggc agagcgttgc ctttgccacc aacacaagcg
     6181 agttcctggg taaaacgtgc gtcaccttat atcgcaatcg cgacgctctg gtacgcggaa
     6241 agctgcacgc gattcgcgga tgagtgcgat cacgaaattc gtcggcgttg cgatcacgaa
     6301 acagtaagac tcagaagcgg taggtcagtg atgaggagta gaagccgaag ccgcgatcga
     6361 caaaagtgtt ctcgaggaag tcgctcgaga agaacctcga gatcacgccg atgagcgtca
     6421 ggtgcggcgt cgcgctccag ctgacgacca cgccggggtt ggtcccgacg aatctgtgat
     6481 cgttggcccg gccgttcagc agcggcctga gatcgatgct gtagagaccg tcgtg
//
LOCUS       DBNGSH010001024         4005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2867586, whole genome shotgun sequence.
ACCESSION   DBNGSH010001024 DBNGSH010000000
VERSION     DBNGSH010001024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2867586"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="AC16CY_23330"
     CDS             <1..544
                     /locus_tag="AC16CY_23330"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR3356805.1"
                     /translation="DNWESPTLGAWGIGWQVLLDGLEITQFTYFQQVGGIDLAPISCE
                     ITYGLERIAMFLQRVDNVFDLEWGGGVKYGEVRLQEEVEQSKYAFGQVDMPGEAFAAL
                     HRELFDKTFDFAGRLLASGLVWPALEHCLKCSHLFNILDSSGSIGVTERTAYVLRIRQ
                     LAVRIAKAYVGEEAPAGESR"
     gene            541..2745
                     /gene="glyS"
                     /locus_tag="AC16CY_23335"
     CDS             541..2745
                     /gene="glyS"
                     /locus_tag="AC16CY_23335"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR3356806.1"
                     /translation="MTGMDRELLIEIGCEEIPASWLPDLTSQLGRHLDARLKQFRLTS
                     EGPTETYSTPRRLTARVAKLAERQTDFEELVTGPPESAAFAADGQPTPAATGFAKKYE
                     VEVGALERHTTPKGTYLAYRVKHRGKATVDVLAEIMTGLLRDLAFPRQMRWDAYLEDG
                     KGDLLFGRPIRWIVLLYGSRVVPFVIRRTEIAQGPTVQDIRSGPNTYGHRFLTTSGRA
                     GRAIKVKTFEDYQGRLLENFVILDRAEREQRIRLELETHARKLGARVGLAAAHATLLQ
                     EVPDLVEYPSVIAGHIPVEFLQLPEEVLTTTMIHHQHFFPVVDDEGRLKPAFLAVTNV
                     QVERPELIARNCERVLIARLRDARFFWDEDRKSTLESRIERLSTILFHKKLGSYREKA
                     DRVSALAAWIAEDLFGRPDAAPRAEQAGRLCKTDLATGMVREMTELQGTMGGIYAREE
                     GLPEDVWKAIYYHYMPVGVEAESPPSRAQLGDAAVTWASVSLADKLDSVVGMFGAGER
                     PTGSRDPLGLRRLAQGAVKILADLPELTGLELRLPLGRLLAQAAVPFGGVGESAGPLH
                     AFMSDRLAYLLEQRGFDVRSIRAVMHGGLEAVSPLDARRKLEALSRMAGSQALLGVAT
                     LLKRVKNISKGVAVPESAGRDRSLLTEPAEQALASALDGSAAGIRTAAARGDYGDAFN
                     GIAALQPVVAKFFDDVLVMAEDERVRAARLGLVASLRGLILEIADISEIVTE"
     gene            2849..>4005
                     /locus_tag="AC16CY_23340"
     CDS             2849..>4005
                     /locus_tag="AC16CY_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3356807.1"
                     /translation="MAKKAVKKSVKRAVTKTTRKAAGKHVYLFGKKTDGNGTMKPLLG
                     GKGANLAEMCRIGLPVPPGFTITTEVCTHYYAHKRSYPSALQGQMKAGIAAIEKQTRK
                     TFGDLKDPLLVSVRSGARDSMPGMMDTILNLGLNDQTVEALARKTGNPRFAWDCYRRF
                     VQMYGDVVLGVQKRPDEDHEPFETVIETLKHERHHEGIEDTRLTVDDLKELVARFKAL
                     VKDRAGKQFPASPWDQLMGAVGAVFGSWMNDRAIVYRRKYNIPSEWGTAVNVQAMVYG
                     NTGDTSGSGVAFTRNPANGAKEFYGEFLINAQGEDVVAGVRTPEPVAELRKHLPKAYQ
                     ELERIRQTLERHFKDVQDFEFTIEEGVVYMLQTRNGKRTAMAALKFSMDMQ"
BASE COUNT          710 a         1267 c         1393 g          635 t
ORIGIN      
        1 ggacaactgg gagtcgccga cgctcggcgc ctggggcatc ggatggcagg tcctgctcga
       61 cgggctcgag atcacgcagt tcacctactt ccagcaggtc ggcggcatcg atctggcccc
      121 gatctcctgc gagattacct acgggctcga acggatcgcg atgttcctgc agcgcgtcga
      181 caacgtcttc gacctggaat ggggcggcgg cgtgaagtac ggcgaggtgc gcctgcaaga
      241 ggaagtcgaa cagtcgaagt acgcgttcgg acaggtagac atgcccggcg aggcgtttgc
      301 cgcgcttcat cgcgagctgt tcgacaagac gttcgatttt gccggcaggc tcctggcttc
      361 cgggctcgtc tggcccgcgc tcgagcactg cctgaagtgc tcgcacctgt tcaacatcct
      421 cgactcgagc ggaagcatcg gcgtcaccga gcggacggcc tatgtgctcc gcatccgcca
      481 gctggcggtc cggatcgcga aggcctacgt cggagaggaa gcgccggcgg gtgagtcgcg
      541 atgacaggca tggaccgcga gctgctgatc gagatcgggt gcgaggagat cccggcgagc
      601 tggctgcccg acttgacgtc gcagcttgga cgccacctcg atgcgcgcct gaagcagttc
      661 aggctgacga gcgagggccc gaccgaaacc tacagcacgc ccaggcgcct gaccgcacgc
      721 gtcgcgaagc tcgccgagcg ccagaccgac ttcgaggagc tggtcaccgg ccctccggag
      781 tcagcggcct tcgcggccga cggccagccg acgccggctg ccaccggatt cgcgaaaaag
      841 tacgaggtcg aggtcggcgc gctcgaacgg cacacgacgc cgaaggggac gtacctggcg
      901 tatcgggtca agcaccgcgg caaggcgacg gttgacgtgc tggcggagat catgaccggg
      961 ctgctgcgcg acctcgcgtt cccgcggcag atgcgctggg acgcatacct cgaggacggg
     1021 aagggcgacc tgctgttcgg gcggccgatc cgctggatcg tcctcctgta cggcagccgc
     1081 gtcgtcccgt tcgtgattcg tcgaaccgag atcgctcagg gtccgacggt ccaggacatc
     1141 cgttccgggc cgaataccta cggccaccgt ttcctgacga cgagcgggcg ggcggggcgc
     1201 gcgatcaagg tgaagacctt cgaggactac caggggcggc tgctcgaaaa cttcgtcatt
     1261 ctcgatcgcg ccgagcgcga acagcgcatc cgcctggagc tcgaaacgca cgcgcgcaag
     1321 ctcggcgcgc gcgtggggct cgccgccgcg cacgccacgc tgctccagga agtgcccgac
     1381 ctcgtcgagt acccgtcagt catcgcgggg cacatcccgg tggagttcct ccagctcccg
     1441 gaggaagtgc tcacgacgac gatgattcac catcagcact tcttccccgt cgttgacgac
     1501 gaaggacggc tgaagccggc gttcctggcc gtgaccaacg tgcaggtcga gcggccggag
     1561 ctcatcgcac gcaactgcga gcgggtgctc atcgccaggc tccgggacgc gcgcttcttc
     1621 tgggacgagg accgcaagtc caccctcgag tcgcgcatcg agcggctgtc gacgattctg
     1681 ttccacaaga agctcgggag ctatcgcgag aaggcagatc gtgtgtcggc gctggcggcc
     1741 tggatcgcgg aggacctgtt cgggcggcct gacgcggcgc cgcgggcaga gcaggcgggc
     1801 cgcttgtgca agaccgacct ggcgaccggc atggtccggg agatgaccga gctccagggg
     1861 accatgggcg gaatctacgc ccgcgaggaa ggcctgcccg aggacgtgtg gaaggcgatc
     1921 tactaccact acatgccggt cggcgtggag gccgagtctc ctccgtcgcg ggcgcagctg
     1981 ggcgacgcgg ccgtgacgtg ggcgtcggtg tctctggccg acaagctcga ctcggtcgtc
     2041 gggatgttcg gggctggcga gcgcccgacc ggctctcgcg atcctctggg actccggcgg
     2101 ctggcccagg gcgccgtgaa gatcctggcc gacttgccgg agctgaccgg tctcgagcta
     2161 cggctgccac ttggccgcct cctggctcaa gctgccgttc ctttcggcgg tgttggcgag
     2221 tctgcgggtc cgctccatgc gttcatgtcg gatcgattgg cgtacctgct ggagcagcgc
     2281 ggcttcgacg tgcgcagcat acgcgccgtg atgcatggag ggcttgaagc cgtcagcccg
     2341 ctcgacgcgc ggcgcaagct ggaagcgctt tcacgcatgg cgggatccca ggcgctgctc
     2401 ggagtagcga cgctgctcaa gcgcgtcaag aacatctcga agggtgtggc cgttcccgag
     2461 tcggccggtc gagaccgcag cctgctgacg gagcccgccg agcaggcgct ggcgtcggcg
     2521 ctggacggca gcgccgccgg gatccgcacg gcggcagcgc gcggcgatta cggcgacgcc
     2581 ttcaacggca tcgcggcgct tcagcccgtg gtggcgaagt ttttcgacga cgtgctcgtg
     2641 atggccgaag acgagcgcgt gcgcgcggcc cgtctcgggc tcgtggcgtc gctgcgggga
     2701 ttgattctcg agatcgcgga tatctcggag atcgtgacgg agtaaatggg gtcgggggtc
     2761 atttaaagag gacactgcgc tttggagtac gtgctgccgc agtgtcctcc ttaaatgact
     2821 cgcgaccccg taggggagac cgaaccgaat ggcgaagaag gctgtgaaga agtctgtgaa
     2881 gagggctgtg acgaagacca cccgcaaggc cgcgggtaag catgtctatc tctttggcaa
     2941 gaagaccgac ggcaacggca cgatgaagcc gctgctcggc ggcaaggggg cgaacctcgc
     3001 cgagatgtgc cgcatcggac tgccggtccc ccccggcttc acgatcacaa ccgaggtctg
     3061 cacgcactac tacgctcaca agcgctcgta tccatcggcg ctccagggcc agatgaaggc
     3121 tggcatcgcc gccatcgaga agcagacgag gaagacgttc ggcgatctga aggacccgtt
     3181 gctcgtctcg gtgcggtccg gcgcgcgcga ctcgatgccg ggcatgatgg acaccatcct
     3241 caatctcgga ctgaacgatc agacggtcga ggcgctcgcc aggaagaccg gcaacccgcg
     3301 ctttgcctgg gactgctacc ggcgcttcgt gcagatgtac ggcgacgtgg tgctcggcgt
     3361 gcagaagcgc ccggacgagg atcacgagcc gttcgagacc gtcatcgaga cgctcaagca
     3421 cgagcgccat cacgagggca tcgaggacac caggctcacg gtcgacgatt tgaaggaact
     3481 cgtcgcccgc ttcaaggccc tcgtcaagga tcgcgccggc aagcagttcc ccgcctcgcc
     3541 ctgggaccag ttgatgggcg ccgtcggcgc cgtgttcggt tcctggatga acgaccgggc
     3601 catcgtctac cgccgcaagt acaacattcc gagcgaatgg ggtaccgcgg tcaacgtgca
     3661 ggcgatggtg tacgggaata cgggcgacac gtccggatcg ggcgtcgcgt tcacgcgcaa
     3721 cccggccaac ggggcgaagg agttctacgg cgagttcctc atcaacgcgc aaggcgaaga
     3781 cgtcgtggcc ggcgtgcgca cgccggagcc ggtggccgag ctgaggaagc acctgccgaa
     3841 ggcataccag gaactggagc gcatccgcca gacgctggag cggcatttca aggacgtcca
     3901 ggacttcgag ttcacgatcg aggagggcgt cgtctacatg ctgcagacgc gcaacggcaa
     3961 gcgcacggcg atggccgcgc tcaagttctc gatggacatg cagaa
//
LOCUS       DBNGSH010001025         2640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3547326, whole genome shotgun sequence.
ACCESSION   DBNGSH010001025 DBNGSH010000000
VERSION     DBNGSH010001025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3547326"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="AC16CY_23345"
     CDS             <1..125
                     /locus_tag="AC16CY_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3356808.1"
                     /translation="RLPEVCANVCFGGPRRNRLFMTASQSLYAVYVETVGAHIA"
     gene            193..>2640
                     /locus_tag="AC16CY_23350"
     CDS             193..>2640
                     /locus_tag="AC16CY_23350"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3356809.1"
                     /translation="MNSEDDPTLLTGAVTAGAPSPVPSSASTAITFAPGSIIAGRYRL
                     VALLGKGGMGEVYRADDLTLDHPVALKFLPSAATGDEATLSQFHNELRVARQVSHKNV
                     CRLYDLGEAGGRRFLTMEYVDGEDLASLIRRIGRLPHDKAVQIARQLCAGVAAAHDRG
                     VLHRDLKPANVMIDGEGDVRITDFGIATATADDTRQFVGTPQYMAPEQFSGGAASVRS
                     DIYALGLILFEVFTGRRAQDSKTLEELKRFHETGTHATPSSIVRDLDPSVERVILRCL
                     ERAPERRPISVLAVAAALPGGDPLAAALAAGETPSPEVLAAAGESEALGVGRGLALVS
                     FVAVALLAFAAASQRTSLTGRTPLDVEPPVLVDRAEQLVSSVGYDEPAGDRAFGFMPF
                     EDYRQWLRRRREPRSTRWDALSSGDPSGVLFWYRSAPGALIPVEDAAVTLTDPPATET
                     GMREVVLDPRGRLQRFRSVPPQFDDSADPARAPAWNMLFDAAGLSMSAFAGATPQWTP
                     PDFADARAAWTGPHPLLQDVTLRVEAASYRGAPVFFEVIGPWTEPERMQPDRTSLADF
                     ILIGVLLGILATLLLAAAVLARHHIRSGRADRRSAWRLTAYLAVVGMVAWLLRADHSL
                     NLEGEVGLLFREAANLSLLSLASWTVYVALEPYVRRLWPDALLGWSRLLTGHIRDPRV
                     GRDLLIGMAFGVALALVDVGKATIIPALGQSAPFPRYGYGDVMLGSGTGALWGTLLES
                     ASAVVAALLATLGIVILRLVLRWRWLAIVVTWIFLSLTTTYDMTAIPMSLAFPLASGA
                     LLSIIAFRFGLLALVVTW"
BASE COUNT          368 a          881 c          896 g          495 t
ORIGIN      
        1 ttcgtcttcc cgaagtgtgc gcgaacgtct gtttcggcgg gccgagacga aacaggctgt
       61 tcatgacggc gagccagtcg ctgtacgcgg tgtacgtcga aacggttggt gcgcacatcg
      121 cctgagcgtg cttcgggact gatcgagcct cacggacgtt aaccacagag acacagagga
      181 cacagagact tcgtgaattc ggaagacgat cccacgctgc tcactggcgc cgtcacggcc
      241 ggggcgcctt ccccggttcc ttcctccgcg tcgacggcca tcacgttcgc cccaggctcc
      301 atcatcgcgg gcaggtatcg cctcgtggcc ctgttgggga aaggcggaat gggggaggtc
      361 taccgcgccg acgatctcac gctcgatcac ccggtcgcgc tgaagttcct tccctccgct
      421 gccacgggcg acgaagcgac actgagccag ttccacaacg aattgcgcgt ggcgcgtcag
      481 gtgtctcaca agaacgtctg ccgcctctac gacctcggtg aagccggcgg ccgccggttc
      541 ctgacgatgg agtacgtcga cggcgaagat ctcgcgtcgc tcatccgccg gatcggccgg
      601 ctgccgcacg acaaggcggt acaaatcgcc aggcaactgt gcgccggcgt ggccgccgcc
      661 cacgatcgag gcgtgctcca tcgcgacctg aagccggcca acgtgatgat cgacggcgag
      721 ggcgacgttc gcatcaccga cttcggcatc gcgacggcga ctgccgacga cacccggcag
      781 tttgtcggca cgccgcagta catggcgccg gagcagttca gcggcggggc ggcctcggtc
      841 cgaagcgaca tctacgcgct cgggctgatc ctgttcgagg tattcaccgg acgccgggcc
      901 caggacagca agactctcga ggagctgaag aggtttcacg agaccggcac ccacgcgacg
      961 ccttcgtcga ttgtccgtga tctcgatccg tcggtcgagc gcgtcatcct tcgatgtctc
     1021 gagcgtgcgc cagaacgccg gccgatctcc gtgttggcgg ttgccgcggc ccttcccggc
     1081 ggcgatccct tggcagcggc gctcgcggcg ggggagacgc cgtcgcccga agttcttgcg
     1141 gcggccgggg aatccgaggc gctcggcgtc ggccggggac tggccctcgt ctcgttcgtg
     1201 gccgtcgccc tgctggcgtt tgccgccgcc tcgcagcgga cgtcgctcac cgggcgtacg
     1261 cctctcgacg tcgaaccacc cgtgctggtc gatcgcgcgg aacagttggt ctcgtccgtc
     1321 ggctatgacg agcctgcggg ggacagggcg tttgggttca tgccattcga ggactaccgg
     1381 caatggctgc gccggcgccg cgaacctcgt tccacgagat gggacgccct ctcgagcggg
     1441 gatccctcgg gcgtgttgtt ctggtatcgc agcgcgcccg gcgccctcat ccccgtcgag
     1501 gatgccgcag tcacgctgac cgatccgccg gccacagaga cgggcatgcg cgaggtggtg
     1561 ctcgacccgc gcgggcggct gcagcgcttt cgatcagtgc cgccgcaatt cgacgacagc
     1621 gccgatccag cccgggcgcc tgcgtggaac atgctgttcg acgccgcagg cttgtcgatg
     1681 tccgcgtttg caggggccac gccgcaatgg actccgccgg attttgcgga cgcccgcgcc
     1741 gcgtggacgg gtcctcatcc gctgctccag gacgtgacgc tgcgtgtcga agcggcgtcg
     1801 tatcggggtg cgccggtctt cttcgaagtg atcgggccgt ggactgagcc cgagcgcatg
     1861 cagcccgacc ggacctcgtt ggcggatttc attctcatcg gcgtgctgct cggtattctc
     1921 gccacgctgc tcttggctgc agcggtgctg gcgcggcacc acatccggtc cggccgtgcc
     1981 gatcggcgca gtgcgtggcg gctcaccgcg tatttggccg tcgtgggtat ggtcgcgtgg
     2041 ctgctgcggg cggatcactc gttgaatctc gagggggaag tcgggctgct cttcagagag
     2101 gctgccaacc tgtcgctgct gtcgctcgcg tcctggactg tgtacgtcgc gctcgagccc
     2161 tatgtcagac ggctctggcc ggacgcgctg ctcgggtggt cccgcctgct gaccgggcac
     2221 attcgcgatc ctcgcgtggg ccgagacctg ttgatcggga tggcgttcgg cgtcgcgctg
     2281 gcgctcgttg acgtcggcaa ggccaccatc attcccgcgc ttggccagtc ggccccgttt
     2341 ccgcgctacg gctacggcga cgtgatgctt ggaagtggaa ctggggcgtt gtggggcacc
     2401 ctgctcgaaa gtgcgagcgc cgtcgtcgcg gcgctccttg ccacgctcgg catcgtgatc
     2461 ctgcggctcg tcctgcgctg gcgctggctg gcgatcgtcg tcacctggat cttcctgtcg
     2521 ctgacgacaa cgtatgacat gacggcgata ccgatgtcgc tcgcattccc gctcgccagc
     2581 ggcgcgcttc tgtcgatcat cgccttccgg ttcggattgc tggcgctcgt ggtgacgtgg
//
LOCUS       DBNGSH010001026         3997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3717350, whole genome shotgun sequence.
ACCESSION   DBNGSH010001026 DBNGSH010000000
VERSION     DBNGSH010001026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3717350"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="AC16CY_23355"
     CDS             <1..295
                     /locus_tag="AC16CY_23355"
                     /inference="COORDINATES: protein motif:HMM:NF017088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR3356810.1"
                     /translation="ELRVPEEALKPLADGAYYVHQLVGCVVETVAGQRVGEVARVEPG
                     SGTSLLAVDGQRGEVLVPLAADICVEVDVAGRRIRIEPPEGLLELNLGRQQAE"
     gene            332..1075
                     /gene="trmD"
                     /locus_tag="AC16CY_23360"
     CDS             332..1075
                     /gene="trmD"
                     /locus_tag="AC16CY_23360"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR3356811.1"
                     /translation="MKFDVVTIFPAMIEAGLGGGVVSRGVERGLVDVAVHDLRRWTRD
                     RHRSVDDVPYGGGPGMVMKAEPLMLAVEEITATRGVPDTVVLLSPQGRTFTQAEAERL
                     ARLDHIVLLCGRYEGMDERVGELVATEELSIGDFVLSGGELAALVVVDAVSRLVPGVV
                     GDEQSVAEDSFSRGLLDHPHYTRPAEIAGKRVPDVLLSGHHAAVRRWRRKAALVRTLD
                     RRPDLIVEAALDDEDRALLDEIRRERSQA"
     gene            1143..1487
                     /gene="rplS"
                     /locus_tag="AC16CY_23365"
     CDS             1143..1487
                     /gene="rplS"
                     /locus_tag="AC16CY_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR3356812.1"
                     /translation="MSAIDLVEKSQMTNRPEMKSGDTVRVHVKVREGDKERIQIFEGI
                     VIGMHRGGVRASFTVRKVSFGQGVERIFPLHSPTIAKVDVIRSARVRRAKLYFLRDLK
                     GKAARMKETKRA"
     gene            1492..2118
                     /locus_tag="AC16CY_23370"
     CDS             1492..2118
                     /locus_tag="AC16CY_23370"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR3356813.1"
                     /translation="MVRVRATRTLENALRRWGFYRIAGCDEVGRGCLAGPVVAGAVVL
                     DPSCHIAGLADSKLLTAAERDRLHDLITTRALAWSVVGLEPAEIDAMNIHRASLEAMR
                     RAVLALAPLPDAVLVDAFRIPDLPMAQRSVLHGDRRCAAIAAASIVAKVVRDRQMITQ
                     HELDPRYGYDRHKGYATAEHRAAVDRFGYSPQHRRSFKPQSGLFDRID"
     gene            2119..3501
                     /locus_tag="AC16CY_23375"
     CDS             2119..3501
                     /locus_tag="AC16CY_23375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356814.1"
                     /translation="MPIDRDATIKKAEKLLKEGKLPAAIREYVGLVEDRPEDWASANA
                     LGDLYVKAGDADRAVEQFLRAADHLHKEGFYPRASAVYKKVLRIKDGHDHTIWRLADI
                     AERHGLLLDARTYYARLIRDRRSRADERGAVDCLVRLALFEDADAEAMLVAARALIPL
                     GESKKAARLMLSAADDLVKHGRATEALDTLKEAAQMSPDDREIRERLSAVASGEGEPV
                     ETPSVGAITLVERDSVEAAPAAPDIAPSPAGADASDEIAAAPPDEDTDIAWTAPEAEP
                     EAPIVPGDAITLSSDAAELSAVLAEIEDLPDPVIEQPRDLESIFEELRAKVARDQDTK
                     ARDQYERALRHLDEGKLAEAIANLEEAARTPTMRFEAASRLARVLVSRGELAAAVDWL
                     EWAVEAPPPTPEEGHAAMYELGDALSRLGETARALAVLMELEGESNGYRDVRTRIAQL
                     SQAQSRGEEM"
     gene            3501..3773
                     /locus_tag="AC16CY_23380"
     CDS             3501..3773
                     /locus_tag="AC16CY_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356815.1"
                     /translation="MRLVLTAFFIETGFVLMLVPWSEFWDRNYFIQGVPLLHALLANN
                     FVRGAVSGLGVINLCAAIAEMYAFFADRRLDEHNVVSLRHTPAAEE"
     gene            3783..>3997
                     /locus_tag="AC16CY_23385"
     CDS             3783..>3997
                     /locus_tag="AC16CY_23385"
                     /inference="COORDINATES: protein motif:HMM:NF014622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3356816.1"
                     /translation="MRHVVCLITDRGRLGSNAEAALIRRVSVAARAGVHLVQIRERDL
                     PDRALLALVTRAVEAARPTRTRVLVNDR"
BASE COUNT          655 a         1251 c         1472 g          619 t
ORIGIN      
        1 cgagctcagg gtgcccgaag aggcgctgaa gccgctcgcc gatggcgcgt actacgtgca
       61 ccagctcgtg ggctgcgtcg tggaaaccgt cgccggccaa cgcgtgggtg aagtggcgcg
      121 cgtggagccg ggcagcggga cgagcctgct ggccgtcgac gggcagcgtg gcgaggtgct
      181 ggtgccgctc gcggcggaca tctgtgtcga ggttgacgtc gcgggacggc ggatacgcat
      241 cgagccaccg gaggggctgc tggagctgaa ccttggcagg cagcaggcag agtagagcgc
      301 acgcttcagc gtgcgcgtgc aagagcgggg aatgaagttc gacgtcgtca cgatctttcc
      361 ggcgatgatc gaggcgggtc tcggcggagg agtcgtgagc cggggcgtcg aacggggctt
      421 ggtggacgtc gcggtgcacg acctgcgtcg atggacgcga gatcgtcatc gaagcgtgga
      481 cgatgtgccg tacggcggcg gacccgggat ggtgatgaag gccgagccgc tgatgctggc
      541 ggtcgaggag atcacggcaa cccgcggcgt gccggatacg gtggtgctgc tgtcgcccca
      601 gggacggacg ttcacgcagg ccgaggccga gcggcttgcc cggctcgatc acatcgtgct
      661 gctctgcggc cgatacgagg ggatggacga acgggtcggc gagctggtgg cgaccgaaga
      721 actgtcgatt ggcgacttcg tgctgagcgg cggcgagctg gcggcgctcg tggtcgtgga
      781 cgcagtcagc cggctggtgc cgggagtggt gggcgacgag caatccgttg ccgaggactc
      841 gttctcgcgt ggtttgctcg accatccgca ctacactcgt cccgcagaga ttgcgggaaa
      901 gcgggtgccg gacgtgttgc tgtcggggca tcacgcggcc gtgcgacggt ggcggcgcaa
      961 ggcggcgctg gttcgtacgc tcgaccggcg gccggatctc atcgtcgagg cggccctcga
     1021 cgacgaggat cgcgcgttgc tggatgagat caggcgcgag cggtcgcagg catgaggtac
     1081 gacggtgcaa gggtgcaacg tagagcgcgg gctcgagccc gcgcggtagt gaggagaagg
     1141 caatgagtgc gattgacctg gtcgagaagt cccagatgac gaatcgaccc gagatgaagt
     1201 ctggggatac ggtacgcgtg cacgtgaagg tgcgcgaagg cgacaaggaa cgcattcaga
     1261 tcttcgaggg catcgtcatc ggcatgcacc gcgggggcgt ccgcgcgtct ttcaccgtgc
     1321 ggaaggtctc gttcgggcag ggggtcgagc gcatcttccc gctgcattcg ccgacgattg
     1381 cgaaggtgga cgtcattcga tccgcgcgcg tgcgtcgcgc aaagctgtac ttcctccgcg
     1441 acctgaaggg gaaggcggcc aggatgaagg aaaccaagcg cgcgtgatcc catggtccgg
     1501 gtgcgcgcca cgaggaccct cgagaacgcc ctcaggcgat gggggtttta ccgcatcgcc
     1561 ggctgcgatg aagtcggccg cggttgcctg gccggccccg tcgttgcagg cgcggtcgtc
     1621 ctggatccgt cctgtcacat cgccggtctc gccgactcga agctgctgac cgcggccgaa
     1681 cgggaccggc tgcacgacct gattaccacg cgcgcgctgg cctggtcggt cgtcgggctc
     1741 gaaccggccg agatcgacgc gatgaacatt catcgcgcgt cgctcgaggc gatgcgccgc
     1801 gcggtgctcg ccctggcccc acttcccgat gcggtgctcg tcgacgcgtt ccggatcccc
     1861 gacctgccca tggcccagcg aagcgtgctc cacggcgacc ggcgatgcgc cgcgattgcc
     1921 gccgcgtcca tcgtggccaa ggtcgtgcgc gacaggcaga tgatcactca gcacgagctc
     1981 gatccccgct acggctacga ccggcacaag ggctatgcga cggctgaaca ccgcgcggcc
     2041 gtcgaccggt tcggatactc gccgcagcat cggcgatcgt tcaagcctca atcggggctg
     2101 tttgatagga tcgattgagt gccgatcgat cgagacgcca cgatcaagaa ggcggagaag
     2161 ctcctcaagg agggaaagct tccggcggcg atccgggagt acgtcgggct cgtcgaagac
     2221 aggcccgagg actgggcctc ggcgaacgcg ctcggtgacc tctacgtcaa ggcgggcgat
     2281 gccgatcgcg cagtcgagca gttcctccgc gccgccgatc acctgcacaa ggaaggcttc
     2341 taccctcgtg cctccgcggt ctacaagaag gtgcttcgga tcaaggacgg ccacgaccac
     2401 acgatctggc gtctggctga catcgccgag cgtcatggcc tgctgcttga tgcgcggacc
     2461 tactacgccc ggctgattcg ggaccggcga agccgggcgg acgaacgggg cgccgtcgac
     2521 tgcctggtgc ggctggcctt gttcgaggac gccgacgccg aggcgatgct ggttgcggcg
     2581 cgcgcgctca ttccgctcgg ggaatcgaag aaggcggcca ggttgatgct gagcgccgcc
     2641 gacgacctgg tcaagcacgg ccgggcgacg gaggccctgg ataccctgaa ggaagccgcg
     2701 cagatgtcgc cggacgaccg agagattcgc gagcgcttgt cggcggtcgc ctcgggcgaa
     2761 ggggagccgg tcgagacgcc atcggtcgga gcgatcactc tggtggagcg ggactccgtg
     2821 gaggccgctc ccgcggcgcc cgatatcgcg ccgtcccccg ctggagcgga cgcgtccgac
     2881 gagatcgctg ccgcgccacc ggacgaagac acggacatcg cctggacagc gccagaagcg
     2941 gagccggagg cgccgatcgt tcccggcgac gcgatcacgc tgtcgagtga cgccgcggag
     3001 ctgagcgcgg tgctcgccga gatcgaggat cttccggacc cggtgatcga gcagccgcgc
     3061 gacctcgagt cgatcttcga ggagctgagg gccaaggtgg cccgcgacca ggacacaaag
     3121 gcgcgcgacc aatacgagcg cgctcttcgg cacctggacg aggggaagct cgccgaggcc
     3181 atcgcgaacc tcgaggaggc cgcgcgaacc cccacaatgc gcttcgaggc cgcctcgcgc
     3241 ctggcccgcg ttctcgtctc gcgcggcgag ctcgccgccg cggttgactg gctcgagtgg
     3301 gccgtcgaag cgcccccacc cacgccggaa gagggacacg ccgcgatgta cgagctgggc
     3361 gatgcgctca gccggctcgg cgaaaccgcg cgggcgctcg ccgtgttgat ggagctcgaa
     3421 ggggagtcga acggctaccg ggacgtacgg acgcggatcg cgcagttgtc gcaggcgcag
     3481 tcgcgaggcg aggaaatgta gtgaggttgg tcctgacggc cttcttcatc gagaccggct
     3541 tcgtgctgat gctggtgccg tggtcggagt tctgggatcg gaactacttc atccagggtg
     3601 tgccgctgct tcacgcgctg ctcgccaaca acttcgttcg aggcgcggta tccggcctcg
     3661 gggtcatcaa cctctgcgct gccatcgccg agatgtacgc cttcttcgcc gatcgccggc
     3721 tcgacgagca caacgtcgtc tcgctcaggc acacgcctgc ggccgaggag taggccggtt
     3781 ccgtgcggca cgtcgtctgc ctgatcacgg atcgcgggcg gctcggctcg aacgccgagg
     3841 ccgcgctgat tcgccgcgta tcggtggccg caagggccgg tgtccacctg gtgcagatca
     3901 gggagcgcga cctcccggat cgtgcgctgc tggcgctcgt gacacgggcg gtcgaagccg
     3961 cgcggccaac ccggacgagg gtcctcgtca acgaccg
//
LOCUS       DBNGSH010001027         1827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4737580, whole genome shotgun sequence.
ACCESSION   DBNGSH010001027 DBNGSH010000000
VERSION     DBNGSH010001027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4737580"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC16CY_23390"
     CDS             <1..390
                     /locus_tag="AC16CY_23390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HIR3356817.1"
                     /translation="GNSVSFHGGSHHQDDPVQIRRYAALNRYHVSTMAYLAEELKSIP
                     DGDGTLLDRTLILYGSNMGNSNQHQHYDVAHLLVGGANGKLKGNRHLKYERKTATTGN
                     LLLSVMDMFDVHKEKQGDSTGRLARLL"
     gene            403..>1827
                     /locus_tag="AC16CY_23395"
     CDS             403..>1827
                     /locus_tag="AC16CY_23395"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3356818.1"
                     /translation="MRTSRAVACAGLLWAVIVGIAAGAGKSDVADAARDGDRAAVQKL
                     ISQKADVNAPQVDGSTALHWAVYRDDAQLLDMLIRARADVNPTTREGVTPLAMASLYG
                     NAAMIDRLIKAGADVKQRGPNGETMVMFAARNGNPQALTVLLEAGAEVNARETVRGTT
                     ALMWAVEQKHPEAVKVLLAAGADPRAKSGGAGLPRNYMANRVNQRAVQLAQDRRKRAA
                     AAGITYEEQLVIDQKTGAEIGGQRGLGQALGPDGQPLAQQPGRQGGAGAAAGAQPPVQ
                     QESPAAAPAAAPDQGQRGGRGRGRGQGAGQTDRTTPQPEGDNDNDDDTEIVVAGLVGS
                     GGGGLTALVFAAREGDTESARLLLDAGAPIDQTTEYGWTPLLTAVNNRNYKTAAFLLE
                     KGADPNLANKGGWTPLYLATDNRNIEGGDYPVPKGDMDHLDIIRLLLEKGANPNAKVK
                     DNTLTRTIFTMQWFFEDGATPFIRA"
BASE COUNT          352 a          598 c          648 g          229 t
ORIGIN      
        1 ggcaacagcg tcagcttcca cggcgggtcg caccaccagg acgatcccgt ccagatacgc
       61 cggtacgcgg ccctcaaccg ctaccacgtc tcgacgatgg cgtacctggc cgaggagttg
      121 aagtcgattc ccgacggcga cggaacgctg ctcgatcgca cgctgattct ctacggttcg
      181 aacatgggca actcgaatca gcaccagcac tacgacgtgg ctcacctcct cgtcggcggc
      241 gccaacggga agctcaaggg gaatcgtcat ctgaagtacg aacgcaagac cgccacgacg
      301 ggcaatcttc tgctcagcgt gatggacatg ttcgacgttc acaaggaaaa gcagggcgac
      361 agcacgggcc gccttgccag gctgctgtag gccgaggcgt acatgagaac ttcgcgcgct
      421 gttgcgtgtg ccggtctgtt gtgggccgtc atcgtcggga tcgcggccgg tgccggcaag
      481 agcgatgtgg ccgacgccgc cagagacggg gaccgcgcgg ccgtccagaa gctgatctcg
      541 cagaaggccg acgtcaatgc gccgcaggtg gacggaagca ccgcgctgca ctgggccgtc
      601 taccgtgacg acgcgcagct gctggacatg ctgattcgcg cgcgggcgga cgtgaacccc
      661 acgacgcgcg aaggggtcac gccgctggcg atggcctcgc tgtacggcaa cgccgcgatg
      721 atcgaccggc tcatcaaagc gggagcggac gtcaagcagc ggggcccgaa cggcgagacg
      781 atggtcatgt tcgccgcgcg gaacggaaac ccgcaggcgc tgacggtgct gctcgaggcg
      841 ggcgccgagg tgaacgcgcg tgagaccgtg cgcggcacga ccgcgctgat gtgggccgtc
      901 gagcagaagc atccggaagc cgtgaaggtg ctgctcgcgg cgggcgccga tccgagggcg
      961 aagtccggcg gcgcgggact tccccgcaac tacatggcga accgcgtgaa ccagcgcgcg
     1021 gtgcagctgg cgcaggaccg ccgcaagcgc gcggcggccg cgggcattac ctacgaagag
     1081 caactcgtca tcgatcagaa gacgggggcg gagatcggcg ggcagcgggg gctcggtcag
     1141 gcgctggggc cggacggcca gccgctggcg cagcaaccgg gccgccaggg tggtgcagga
     1201 gccgcagccg gtgcgcagcc gcccgtgcaa caagaatcac ccgcggcggc gcctgcagcg
     1261 gcgcccgacc aaggccagcg aggcggccgt gggcgcggcc gtggacaggg agccgggcag
     1321 acggatcgga ccactccgca acccgagggt gacaacgaca acgacgatga caccgaaatc
     1381 gtcgtcgccg gcctcgtggg cagcggcggc ggcggcctga cggcgctcgt gttcgcggca
     1441 cgcgagggcg acaccgagtc ggcgcggctg ctgctcgacg ccggcgcgcc gatcgaccag
     1501 acaaccgaat acggatggac gccgctcctg acggccgtca acaaccgcaa ttacaagacc
     1561 gccgcgttcc tgctcgagaa gggcgcggat ccgaacctcg cgaacaaggg cgggtggacg
     1621 ccgctctatc tggccaccga caaccgcaac atcgagggcg gcgactatcc ggtccccaag
     1681 ggggacatgg atcatctcga catcattcgc ctcctgctcg agaagggcgc gaacccgaac
     1741 gcgaaggtca aggacaacac gctgacgcgc acgatcttca cgatgcagtg gtttttcgag
     1801 gacggcgcca cgccgttcat ccgcgcg
//
LOCUS       DBNGSH010001028         3408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1849893, whole genome shotgun sequence.
ACCESSION   DBNGSH010001028 DBNGSH010000000
VERSION     DBNGSH010001028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1849893"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..400
                     /locus_tag="AC16CY_23400"
     CDS             8..400
                     /locus_tag="AC16CY_23400"
                     /inference="COORDINATES: protein motif:HMM:NF041373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGGxSTG domain-containing protein"
                     /protein_id="HIR3356819.1"
                     /translation="MRHGNRPGDPSTAPWCGAQTRRNQPCRAPAVHGRRRCRLHGGRS
                     TGPRTPEGLKRSVRARWKHGRYSAERRREFQRLQAEYAAFNVAAAARHAWVIAAAKLL
                     LREEAKAARNARRRQRRTQQHVRLNRGT"
     gene            complement(823..1647)
                     /gene="istB"
                     /locus_tag="AC16CY_23405"
     CDS             complement(823..1647)
                     /gene="istB"
                     /locus_tag="AC16CY_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR3356820.1"
                     /translation="MLTSTVPPPTEQLTTLLTTFGLTTAAEEIVPRLTQAGHAASVPA
                     LVEVFETEADVRRQRRIARLRRAARLPPGKTFETLDAGRLPLPIMQRLQELATGTFLE
                     TATNILAFGLPGVGKSHALCAVGHALVEAGHTVLFTPAYSVVQELLSAKRDLELPRAL
                     RKLDLFEVILLDDLGYVQQSPEEAEVLFTLLAERYERRSVMVTSNLIFSQWDRIFRDQ
                     MATAAAIDRLVHHAVLLEFDVPSYRTDRSRPTPSAPAPKASRRGRASGPLLPKRRR"
     gene            complement(1641..3086)
                     /gene="istA"
                     /locus_tag="AC16CY_23410"
     CDS             complement(1641..3086)
                     /gene="istA"
                     /locus_tag="AC16CY_23410"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR3356821.1"
                     /translation="MDGNTVGAAAAAAGMCERTARHWQEGPFPSATKTARTWRTREDP
                     FEGVWATEIVPRLAADEDRRLQALTLFEWLSERHPGRFQPGQVRTLQRRVRDWRAQHG
                     SDVQAYFEQTAVPGREGALDFTDATGLGVTIQGEPFPHLLFEWVLSFSGWTYVALAAS
                     ETFEALVAGLQGAIWTLGAAPAVVRHDNLSAATHELKRSGGRQLTERFRGVLDHYGLR
                     SSRIQPGEAHENGVVEQAHFRTKTAVEQALLLRGHAEFGDEATYEGFVRDVIDRKRNR
                     PAAARLAEERPYLRALPPAPVPNYTTFTSVVRRWSTIRVGGRTYSVPSRLLGHTIEAR
                     QHPTTVEVLYRGHVIETMPRLRGEAEHRIDYRHIIGSLVRKPGAFARYRYREELFPSL
                     TFRQAYDALGVTHGERADVEYVRLLHLAATTSERHVETTLLALLEDRQPFDYAGVQAL
                     VTPTTTRVPAIHIPTPDLAQYDALLAGGGRC"
     gene            complement(3212..>3408)
                     /locus_tag="AC16CY_23415"
     CDS             complement(3212..>3408)
                     /locus_tag="AC16CY_23415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356822.1"
                     /translation="YEGFYQLQTESIQLIRDTLGEARILLFIRRQDAHANSWYNQLIK
                     AHRVPIFEIEEFERRVINDN"
BASE COUNT          584 a         1116 c         1110 g          598 t
ORIGIN      
        1 cggctggctg aggcacggca accgaccggg cgacccctcg acggcaccgt ggtgcggggc
       61 acagacacgc cggaatcagc cctgtcgagc cccagccgtt cacggccgac ggcgctgtcg
      121 gctgcatggc gggcgaagca ccggaccccg cacgcctgaa ggtcttaagc gcagcgtgcg
      181 ggcacgctgg aaacacgggc ggtattcggc tgagcgacga cgtgagttcc agcgcctgca
      241 ggcggaatac gcggcgttta atgtggccgc ggcggcgcgc catgcttggg tgattgctgc
      301 cgccaagttg ttacttcgtg aggaagcgaa agccgccaga aacgcgcgac gacgtcaacg
      361 gcggacgcag cagcatgtgc gcctcaatcg ggggacttga cctccttctc gtcgattatc
      421 gcgattaatc gacgatcgca tgatcgacgt caactacttt tgccggttgg cgacaactat
      481 tcttgccggt cctgaatgtg gccgaggctg cgacgatcct gcggccggtt gaatctgagg
      541 acgaggtcgc tctcggtcag tctcggtgtc cttttctcct ttggataaga aggatgagga
      601 tggcttgtgg gaatgtggga atctcgcgtt ctgtgcgaga tttccaagtc cctgtggaaa
      661 ccgtttttgg gtttccatag ggacgtcatt tccacagccg ttttcgcgtt cgcggtttgc
      721 gaggccggca tccggggggc tgtataccct ggccggcccg tcgatcgtcg ctcctggcgc
      781 ctccgacgcg gtcgtttgtg atcggccgac tgatcgaatc gctcatcggc gccgtttcgg
      841 cagcaagggt cccgacgctc gcccccgtcg cgacgctttc ggcgcgggcg ccgacggcgt
      901 cgggcgtgag cgatcggtgc gatagcttgg gacatcgaac tcgagcagca ccgcgtgatg
      961 cacgaggcga tcaatcgcgg ctgccgtggc catctgatcg cgaaagatcc ggtcccactg
     1021 actgaagatc agattgctcg tgaccatcac ggagcggcgt tcataacgct cggcgaggag
     1081 cgtaaagagg acttcggctt cctcgggact ctgttgcacg tagccgagat cgtcgagcag
     1141 gatcacttcg aagagatcga gcttccggag cgcgcgcggc agttccagat cgcgcttcgc
     1201 gctcagcagc tcctgcacca cactgtaggc gggcgtgaag agcacggtgt gccctgcctc
     1261 gaccaaggcg tgaccgacgg cacagagcgc gtgacttttc ccaacgccgg ggagaccgaa
     1321 cgccaggata ttggtcgcgg tctcaagaaa cgtcccggtc gcaagctcct gcaaccgctg
     1381 catgatcggc agcggcagcc ggccggcgtc gagcgtctca aacgtcttgc ccggcgggag
     1441 ccgcgcggct cggcgcaatc gcgcaatccg ccgctgccgc cggacgtcgg cctctgtctc
     1501 aaacacctcg accaacgccg gcacgctcgc ggcgtggcca gcctgcgtca atcgcggcac
     1561 aatttcctcc gcggcggtcg tcaagccgaa ggtcgtgagg agcgtggtca actgctcggt
     1621 gggcggtggc acggtggacg tcagcatcgg ccaccgcccg ccaggagcgc gtcgtactgc
     1681 gcgagatcag gcgtcggaat gtggatcgcg gggacgcgcg tggtcgtcgg agtcaccagc
     1741 gcctgcaccc cggcatagtc gaacggctgc cggtcctcga gcagggcgag caacgtcgtc
     1801 tcgacgtgcc gctcgctggt ggtggcggcc agatgcagca gccgcacata ctcgacgtcg
     1861 gcgcgctcgc cgtgcgtgac gccgagcgcg tcgtacgcct ggcgaaacgt gagggaggga
     1921 aagagctcct cccgatagcg ataccgcgca aacgcgcccg gcttgcggac caagctcccg
     1981 atgatgtggc ggtaatcgat gcggtgctcg gcctcgccgc ggagccgcgg catcgtttca
     2041 atcacgtggc cgcgatagag gacctccaca gtcgtcggat gctgccgcgc ctcgatggtg
     2101 tgtccgagca accgcgacgg cacggagtac gtccgcccgc cgacgcggat cgtgctccac
     2161 cggcgcacca cgctcgtaaa cgtcgtgtaa ttggggacag gcgccggcgg aagcgcgcgc
     2221 aagtacggac gctcctcggc gagtcgggcc gccgccggcc gattccgctt gcggtcaatc
     2281 acgtcccgca cgaagccctc gtaggtcgct tcgtcaccga actccgcatg accgcgcagc
     2341 agcaacgcct gctccactgc cgtcttcgtg cggaaatggg cctgctcgac cacgccgttt
     2401 tcgtgtgcct caccgggctg aatgcgcgag gagcgtaaac cgtaatgatc gaggacgccc
     2461 cggaagcgct cagtgagctg acggccgccg ctccgcttca attcatgcgt ggctgccgag
     2521 agattgtcgt ggcgaacgac ggcaggggcc gcgcccagcg tccagatcgc gccctgcaac
     2581 ccggccacca gcgcttcaaa cgtctcgctc gccgccagcg cgacgtaggt ccagccgctg
     2641 aagctgagga cccattcgaa caagagatgc gggaagggct cgccctgaat ggtgacgccc
     2701 aatccagtcg catcggtgaa atcgagcgca ccctcgcggc cggggacggc cgtttgctca
     2761 aagtacgcct ggacatccga accgtgttgc gcgcgccagt cgcgcacgcg acgctgcaac
     2821 gtgcgcacct ggcccggctg aaaccgtccc gggtgccgct cagataacca ctcaaacagc
     2881 gtcagcgcct gcagccgccg gtcttcatcc gctgccagcc ggggcacgat ctccgtggcc
     2941 cagacgcctt cgaacggatc ctcgcgcgtc cgccaggtcc gcgccgtctt ggtggccgag
     3001 ggaaacggtc cctcctgcca gtgccgggcc gtccgctcac acatgccggc cgccgcagcg
     3061 gccgctccaa cggtgttgcc gtccatccgc ttctccctta accgccgaac ctgcgcgtcg
     3121 gtgaccaatc cgtcctccgt gacggacagc gtcgacgccg aaattcggcg ggtagcggac
     3181 cggcaaatct aattgtcgtc ggccggcaaa tttaattgtc gttgatcacc ctccgctcga
     3241 attcctctat ctcgaagata ggcactcgat gcgccttgat gagctggtta taccaggagt
     3301 tcgcgtgcgc atcctgccgc cggatgaaga ggaggattcg cgcttcgccg agcgtgtccc
     3361 gtatgagctg tattgattcg gtctgcagct ggtagaagcc ttcgtacg
//
LOCUS       DBNGSH010001029         2912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3038894, whole genome shotgun sequence.
ACCESSION   DBNGSH010001029 DBNGSH010000000
VERSION     DBNGSH010001029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3038894"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC16CY_23420"
     CDS             complement(<1..225)
                     /locus_tag="AC16CY_23420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356823.1"
                     /translation="MRRIALVVATAAACALGTHTAGAQQQFKVFASGADAAGNPVPTL
                     ETTDLKIQESGLDAKIVAVEPINWPTKLQVL"
     gene            complement(239..1195)
                     /locus_tag="AC16CY_23425"
     CDS             complement(239..1195)
                     /locus_tag="AC16CY_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3356824.1"
                     /translation="MTSARYLVTGGAGFIGSHLCEELVRRGHHVRAVDNLITGHRQNL
                     AHVPQVDFLEGDLADLDVARRATNGIDYVLHQAAIPSVPRSVEDPITSNRANVDASLN
                     LLVAARDAGVRRVVYAGSSSAYGDAPSLPKVETMATAPLSPYALQKLVAEDYARMFTR
                     LYGLETVTIRYFNVFGPRQDPSSPYSGVISLFISALCEGRRPTIFGDGEHTRDFTYVA
                     NVVDGVLRACDAPGVSGEVINVATGGRISLNRLFETLRRLIGADVEPIYRDARPGDVR
                     HSQADIGKAQRLLGYQPLVSFEEGLGRTLAWYRQMASAASRT"
     gene            complement(1192..2367)
                     /locus_tag="AC16CY_23430"
     CDS             complement(1192..2367)
                     /locus_tag="AC16CY_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3356825.1"
                     /translation="MALMTGRALLAVLVSIAAASVIVAQAPSGRQSEGALQARPGADR
                     QPEFPVPDIRQYKPKSSLVVPEHPTPRAKYPVIDLHSHHNLPLQAADFKRVVTSMDAL
                     NLRVLVNSTGESGAGLDEALRSIRASGYADRIVQFADVNFSRVAPGFGPAAARQLEAD
                     VKAGALGLGELSKNLGLSARKADGTRLKIDDPELDPIWETAARLNIPVLIHTADPEKF
                     WEPIDYANERWLELALYANRRYQDSRFPSFETLMGERDRLFRRHPKTTFVAAHLGWHG
                     NNLARLGKMLDEMPNLYTEVGAVLYDLGRQPRAAREFLVKYQDRVLFGKDNYQPDEFP
                     YYWRTFETADEYFDYYRDYHAFWKLYGLSLPDEVLRKLYYQNALKIVPGMPRTGFPK"
     gene            complement(2485..>2912)
                     /gene="rnpB"
                     /locus_tag="AC16CY_23435"
     ncRNA           complement(2485..>2912)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16CY_23435"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          454 a          956 c          975 g          527 t
ORIGIN      
        1 gagcacctgc agcttcgtgg gccagttgat cggctcgacc gcgacgatct tggcgtcgag
       61 cccggattcc tggatcttga ggtccgtcgt ttcgagggtc ggcacggggt ttccggccgc
      121 gtccgccccg ctggcgaaca ccttgaactg ctgctgcgcg cctgccgtgt gggtgccaag
      181 ggcacacgcg gctgcagtgg cgacgacgag ggcgatccgg cgcatgagca acctccgatc
      241 aggtccgtga cgccgccgag gccatctggc ggtaccacgc aagggtacga ccgagtcctt
      301 cctcgaagct cacgaggggc tgatacccga gaaggcgttg tgccttgccg atgtcagcct
      361 gagagtgtcg cacgtctccc gggcgggcgt cccggtagat cggctccacg tctgccccga
      421 tcagacggcg aagtgtctcg aacagccggt tgagggaaat gcgcccgccc gtggcgacgt
      481 tgatgacttc cccgctcacg cctggcgcgt cgcacgcgcg cagcacgccg tccacgacgt
      541 tggccacgta ggtgaagtct ctcgtatgct cgccgtcccc gaagatcgtg ggccgccgcc
      601 cctcgcacag cgcgctgatg aagagcgaga tgacgcccga ataaggcgac gacgggtcct
      661 gccggggacc gaacacgttg aagtagcgga tggtcacggt ttcgaggccg tacaggcgcg
      721 tgaacatcct ggcgtagtct tcagcgacca gtttctggag cgcatacggc gatagcggcg
      781 ccgtagccat cgtctccacc ttcggcagcg acggcgcatc accgtaggcc gacgatgagc
      841 ccgcgtagac gacgcgccgg accccggcgt cgcgggccgc caccagcagg ttcagcgacg
      901 catcgacgtt ggcgcggttc gacgtgatgg gatcctcgac cgagcgcggg acggagggga
      961 tcgcggcctg atggagcacg tagtcgatac cgttcgtcgc ccgcctggcg acgtcgagat
     1021 cggccaggtc gccttcgaga aagtccacct gcggcacgtg ggccaggttc tggcgatgtc
     1081 ccgtgatcag gttgtccacg gcacggacgt gatggccgcg gcggacgagt tcctcgcaca
     1141 ggtgggagcc gatgaatccg gcgcccccgg tcacgaggta gcgggcggat gtcacttcgg
     1201 gaatccggtg cgaggcatgc cgggaacgat cttcaaagcg ttctggtagt acagctttct
     1261 gagcacctcg tcgggcagcg acagcccgta cagcttccag aacgcgtgat agtcgcggta
     1321 gtaatcgaag tactcgtcgg ccgtctcgaa cgtgcgccag tagtacggga attcgtcggg
     1381 ctggtagttg tccttgccga acaggacgcg gtcctggtat ttcaccagga attcgcgggc
     1441 ggctcgcggc tgccggccaa gatcgtacag gacggcgccc acctccgtgt agaggttcgg
     1501 catctcgtcg agcatcttgc cgagcctcgc caggttgtta ccgtgccagc caaggtgcgc
     1561 ggcgacgaag gtcgtcttcg gatgcctcct gaacaggcga tcgcgctccc ccatcagcgt
     1621 ctcgaagctg gggaacctgg aatcctggta ccggcggttg gcgtagagcg ccagctcgag
     1681 ccatcgctcg ttcgcgtagt cgatgggctc ccagaacttc tccggatcgg ccgtgtggat
     1741 caacacgggg atgttgaggc gcgcggcggt ttcccagatc ggatcgagct cggggtcgtc
     1801 gatcttcagc cgcgtaccgt ccgcctttcg agccgacagg cccaggttct tcgacagctc
     1861 gcccaggccg agcgcgccgg ccttgacgtc ggcttcgagc tgacgggcgg ccgccgggcc
     1921 gaagccggga gcgacgcggc tgaagttgac gtccgcgaac tgcacgatcc ggtcggcgta
     1981 cccgctggcg cgaatcgatc ggagggcctc gtcgaggccg gcccccgact cgcccgtcga
     2041 gttcacgagt acgcgcaggt tgagcgcgtc catcgaggtg acgactctct tgaaatcagc
     2101 cgcctggagc ggcaggttgt gatggctgtg gagatcgatg acggggtatt tcgcccgcgg
     2161 tgtcggatgc tcgggtacca ccagcgacga tttcggcttg tactgccgga tgtcgggaac
     2221 cggaaactcc ggctggcgat cggcgcccgg ccgcgcctgc agggccccct cgctctggcg
     2281 gccgctcggc gcctgggcga cgatcactga agctgcggca atactgacga gaactgcaag
     2341 aagggcccgg ccggtcatga gtgccattgt aactatcccg agccccgaat ccctgattcc
     2401 tggttctgag gcccgggccc tgaggtctgg accctgaaac ctgggccctg aggcctggcc
     2461 cctgaggcct gaggcctttg ataaagccgc agtcagccga taagccgaat tctgttccac
     2521 ctccgcccgt tcgggctacg gcggacaagc ccttgcgagc ctgcccaccg aagcgcgcga
     2581 cgcgcgcgaa ggtgggacga ccattcctct aggccggtga tcgctcaccg gctccagcga
     2641 cctacccggt ggcctcggac gggccgtcct cagcgccacc ctatttggtc ttgctccgtg
     2701 cggggtttgg cctgccgccg tcgttgccgc cggcgcggtg cgctcttacc gcaccttttc
     2761 acccttaccc ctcgactcgg cgcgtccttg cggacgcgcc tcgctcgggg cggtatgttt
     2821 tctgtgccac tgtccgtcag gttgccctgc ccgggcgtta cccggcacac tgcccttcgg
     2881 agttcggact ttcctcccgc ctccgtcctt cg
//
LOCUS       DBNGSH010001030         2159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4059740, whole genome shotgun sequence.
ACCESSION   DBNGSH010001030 DBNGSH010000000
VERSION     DBNGSH010001030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4059740"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2159
                     /locus_tag="AC16CY_23440"
     CDS             <1..>2159
                     /locus_tag="AC16CY_23440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356826.1"
                     /translation="GLSESEARRQVSLELGGAAQVQEAVRDAWTYRWLDALARDVRHA
                     TRSLRRSWGFTFGAGAVLSLAIGANVAIFSVVNAVLLQPLPYPDAGRIVAIETLWTNT
                     GVASQAVSGPDFLDWQAQNDVFEKMAAYYSGDTGGTDDAPVILGDRATFANPRYVSAD
                     FFDVFGQTPSAGRLLTGGDYSVPRGGIPVAVVRHEWAVGHFGSAEAAIGKRLTVNGGP
                     LEIVGVAAPGFRYPGATDLWAPFAPDGDPHDRGGHDYHAVGKLKAGAELARAQAQMRT
                     IGDALARQYPGNRLKTVSLTPLQERLTGNLQATLWVLLGAVGVVWLIGCANIANLLLA
                     RAAGRTREIALRAALGAGRARVVRQLLTESCVLAGTAGLAGLLLASVLMKGVVAFAPA
                     DLPRLDEVRMDMTVAFFAIGLSLVSTVLFGLVPALHASRLNVSDGLKQGGSKATASKT
                     GTRLRSTMVVAEVALSVVLLAAAGLLLRSFLTLQHQDLGFTTDRVLVAFTEHPVKDRI
                     RFFADVLDRLRAVPGVSAASGVALLGMGVEPRPPRDYFIDGRPEGRSGERAQAEFHDI
                     TGDYFRTLEIPMRAGRDFSRTDTVDGPRVAIINEALARTAFAGESPIGRRIKENPDPR
                     VPWMEIVGVVGDARWQDPSQPAPPVVYRASTQGTGNSLSILARTSLDPTALAGTLRTF
                     MYEANPTVPVKFETMEALFDDTLAYPRFRTQVIGLFAG"
BASE COUNT          334 a          706 c          745 g          374 t
ORIGIN      
        1 ggcctgagcg agtccgaggc ccggcggcag gtcagcctcg agcttggcgg cgccgcgcag
       61 gtgcaggagg ccgtacggga tgcgtggaca taccgatggc tcgatgcgct cgcacgcgac
      121 gtccgccacg cgacgcggag ccttcgcagg agctggggat tcaccttcgg cgcgggcgcc
      181 gttctttccc tggccatcgg cgccaacgtc gccattttct cggtggtcaa tgccgtgttg
      241 cttcagccgc tcccctatcc tgacgccggg cgcatcgttg cgatcgagac gctgtggacc
      301 aacaccgggg tcgccagcca ggccgtgtca ggtcccgact ttctggactg gcaggcgcag
      361 aacgacgtgt tcgagaagat ggccgcgtac tacagtggag acaccggtgg taccgacgat
      421 gccccagtca tccttggaga ccgcgccacg tttgccaacc cccgatacgt ctcggccgat
      481 ttctttgacg tcttcggtca gacgccgtcg gcgggccgcc tgctgaccgg aggtgactac
      541 tcggtgcccc gggggggaat cccggtcgcc gtcgtacgac atgagtgggc cgtggggcac
      601 ttcggaagcg ccgaggccgc gatcgggaag cggctcacgg tgaacggcgg gccgctggaa
      661 atagtggggg tggcagcacc agggtttcgt tatcccggcg ccaccgatct gtgggcgccg
      721 ttcgcgcccg acggagatcc gcacgaccga ggtggtcacg attaccacgc cgtcgggaag
      781 ctgaaggctg gcgcagagct cgcgcgggca caggcgcaga tgcggaccat tggcgatgcg
      841 cttgcgcggc agtatccggg gaatcgcctg aaaaccgtca gcctgactcc gctgcaggag
      901 cggctgaccg gaaatctcca ggcgacctta tgggtcctgt tgggcgccgt gggcgttgtc
      961 tggcttatcg ggtgcgcgaa tatcgcgaac ctcctgctgg cgcgggcggc aggccggacg
     1021 cgcgaaatcg cgctgcgcgc cgcgctcggc gccggccgcg cccgcgtggt gcgccaactg
     1081 ctcaccgaaa gctgcgtgct cgcggggacg gccggactgg ccggcctcct gctggcgtcc
     1141 gtgcttatga agggagtcgt cgcgttcgca cccgccgatc tgccccgcct cgacgaggtg
     1201 cgaatggaca tgaccgtcgc tttcttcgcg atcggactct cgctggtatc cacggtgctc
     1261 ttcggcctcg tgccggcact acatgcctcg cggctgaacg tgtcggatgg gttgaaacag
     1321 ggcggttcga aggcgacggc ctcgaaaacc ggcacgcggc tccgctcgac gatggtcgtc
     1381 gccgaagtgg cattgtcggt tgtgctcctg gcggccgccg ggctgctgct gcggtcgttc
     1441 ctgacgctcc agcatcagga cctcgggttc acgacggatc gggtactggt agccttcacg
     1501 gagcacccgg tgaaggaccg gatccgtttc tttgcagacg tgctcgaccg cctccgtgcc
     1561 gtcccaggcg tcagcgcggc gtcgggcgtc gccctcctcg ggatgggagt cgagccgcga
     1621 ccacctcgcg actatttcat cgacggccga ccggaggggc gatccggcga gcgggctcaa
     1681 gccgagttcc acgacatcac gggcgactac ttcaggacgc tggagattcc catgcgtgcc
     1741 gggagggact tctccaggac cgacacagtc gatgggccgc gagtcgccat catcaacgaa
     1801 gccctggcgc gtaccgcatt tgccggggag tcgccgatcg gacggcgaat caaggagaac
     1861 cccgatccca gggtgccctg gatggagatt gtgggcgtgg ttggcgacgc ccgctggcag
     1921 gatcccagtc aacccgcgcc gccggtggtc tacagagcct cgacgcaagg cacgggcaac
     1981 tccctgtcca tcctggcgcg aacctcgctc gacccgacgg cgcttgccgg cacgcttcgc
     2041 acgttcatgt acgaggccaa tcccaccgtg cccgtgaagt tcgagacgat ggaggcactc
     2101 tttgacgaca cgctggcata tccccggttc cgcacgcagg tgattggact gtttgccgg
//
LOCUS       DBNGSH010001031         3988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1852376, whole genome shotgun sequence.
ACCESSION   DBNGSH010001031 DBNGSH010000000
VERSION     DBNGSH010001031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1852376"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="AC16CY_23445"
     CDS             complement(<1..211)
                     /locus_tag="AC16CY_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3356827.1"
                     /translation="MDLAVSVRNLSVQYGRRLVFQGVSFDLAAGQAAGVIGPNGSGKT
                     TLLRTIAGCLTPRSGEVRINGLTPRD"
     gene            complement(211..915)
                     /locus_tag="AC16CY_23450"
     CDS             complement(211..915)
                     /locus_tag="AC16CY_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356828.1"
                     /translation="MTDTPYLLRFFQVVPPVPPLIVATFVAVTGVASLAVATSPGRAR
                     GALAPVLLLQLFTASSGFAVPARRGHYDLLLTRGTSRLRVTLAHWVTTIAPGVFSWLV
                     LAVVERLAVGDGAVLLASGTLAAIFMVSTLPWALTIALPRFSGGIGWLLLLVTMGTTF
                     AEGVFAEWTVTSTRVEALVWPAWVFLIYPLGAVGESLGPPQLLAVAPGITLAAAAMAI
                     ACHWAAHSDIPLSAAQ"
     gene            complement(917..2347)
                     /locus_tag="AC16CY_23455"
     CDS             complement(917..2347)
                     /locus_tag="AC16CY_23455"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3356829.1"
                     /translation="MGETFGAVQRDGVPCPRAYVSIASLRQVLEALGAALVRNTTDKD
                     LEATFEQEVRQLLDLRAVRLREVRVRYQARLVTPTRTADSIVLGVPTADPRVQAVLEA
                     SCDPAKPLDDPDFEALITLAHLGGLVLEVARGRALFGNKPRPSPGQLIGSTLVMGALR
                     EQVERVAATDFTVLIEGESGTGKELVARQLHDLSRRRRGPFVALNCAAVVETLLEAEL
                     FGIEERTATGVRGRRGKFEHAEGGTLFLDEVSDLSLSAQAKLLRVLQDLAVERVGGQG
                     VHRIDTRIVAASNRPLAELVARGQFRPDLYYRLSGVEIHVPPLRARRDDVPELARYFL
                     GRHRNTRDLTLSPEAEQALTIYPWPGNVRELERLIERSLALAESDRIEIDDLPRRIRG
                     DFKEILLPSLARDESMRAWGSQYARLVFDRCGGNKRQACRILKISYHTLDAYLRVAGW
                     RGRGGGRYLPRWVKSVSDSQPGSSGN"
     gene            2677..3843
                     /locus_tag="AC16CY_23460"
     CDS             2677..3843
                     /locus_tag="AC16CY_23460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3356830.1"
                     /translation="MSEKPVEVSVVIPCLNEADTLATCIRKASDGMARAGINGEIVVA
                     DNGSTDDSPAIARAAGARLVPVAERGYGAALMGGIDAARGRFIVMGDADDSYDFGEVP
                     AFVERLREGFDLVQGCRLPSGGGRVLPGAMPPLHRWFGNPMFSRVARAWFRAPVHDIY
                     CGLRGFTKALYERLDQRCTGMEFATEMIVKASLNGARISEVPITLHPDGRRTHAPHLK
                     TFRDGWRTLRFFLMCTPRRLFLMPGLVLLVLGLIGYTVAMPGVTLGGLTFDAHTLLFA
                     SLAVICGYQSMLFSLIAKTFAASEGLLPADRRVERFASILPLERSLLAGLGTVAIGGV
                     MLAAALNEWRVLNFGRLDYAHTMRVVVPGVTLSVLGYQTILSSFFLSLLGMRRR"
BASE COUNT          673 a         1364 c         1310 g          641 t
ORIGIN      
        1 catctcgagg cgtcagcccg ttgatgcgaa cttccccgga cctcggcgtg aggcagccgg
       61 cgatcgtcct cagcagcgtc gtctttcccg acccgttcgg gccgatgacg ccggcagcct
      121 gccccgctgc gagatcgaag gagacgccct ggaacacgag gcgccggccg tattgaaccg
      181 agagattccg gaccgatacc gcaaggtcca tcactgtgcg gccgacagag gaatgtccga
      241 gtgtgcggcc cagtggcacg caatggccat cgcggccgcg gccagggtga tgcccggcgc
      301 cactgccagc agttgcggcg gtccgagcga ctcacctacc gcaccgagtg gatagatcag
      361 gaacacccac gcgggccaga cgagcgcttc gacgcgcgtg gaggtcacgg tccattcggc
      421 gaacacgcct tcggcaaacg tcgtccccat ggtcacgagc agcaagagcc agccgatgcc
      481 gccggaaaag cgcggcagag cgatcgtcaa cgcccacgga agcgtagaga ccatgaatat
      541 ggcggccagt gtcccggagg cgagcaatac ggcgccgtct ccgacggcga ggcgttcgac
      601 aacggcgagc accagccagc tgaagacccc gggcgcaatc gtcgtcaccc aatgcgcgag
      661 ggtgacgcga aggcggctgg tgccccgggt cagcagcagg tcgtagtgac cgcgccgcgc
      721 cgggacggcg aaccccgacg aagccgtgaa caactggagg aggagcacgg gagccagcgc
      781 tcccctcgct cgtccggggg aggtcgccac agcaaggctc gcgacgccgg tgacggcgac
      841 gaaggtcgcc acgatcaacg gcggaaccgg cggaacgacc tgaaagaaac gcagaaggta
      901 cggagtgtcc gtcacgtcag tttcctgaac tccccggctg cgaatcgctg accgatttca
      961 cccaccgggg cagatacctg cccccgccgc ggccgcgcca gcccgcgacc cggaggtacg
     1021 cgtcgagcgt gtgatagctg atcttcagga tgcggcacgc ctggcgcttg ttgcctccgc
     1081 accggtcgaa gacgagccgc gcatattggc ttccccacgc ccgcatcgat tcgtcccgcg
     1141 ccagtgacgg cagcaggatc tccttgaagt ccccgcgaat gcggcgcggc aggtcgtcga
     1201 tctcgatgcg atccgattcc gccagcgcca gcgaccgctc gatgagccgt tcgagttcac
     1261 gcacattgcc cggccacgga tagatcgtca gcgcctgctc ggcttcgggc gagagtgtca
     1321 ggtctctcgt gttcctgtga cgtcccagaa aatatcgcgc cagctccggc acgtcatcgc
     1381 gccgcgcgcg caggggcgga acgtgaatct ccacaccgct cagacggtag tacaaatccg
     1441 gccggaactg gcctcgcgcc accagctccg cgagagggcg gttggacgct gccacgatgc
     1501 gcgtgtcgat acgatgcact ccctgtccgc caacgcgctc gaccgcgaga tcctgcagaa
     1561 cgcgcagaag cttggcctgg gccgacagcg acaggtcaga cacctcgtcg aggaagagag
     1621 tgccgccctc ggcgtgctcg aacttgccgc gacgccctcg aacaccggtt gccgtgcgct
     1681 cctcgatgcc gaacagctcc gcttcgagca gcgtctcgac caccgcggcg cagttgagcg
     1741 cgacgaacgg gccccgccgg cgcctgctca agtcgtgaag ctgtcgcgcc acgagctcct
     1801 tgccggtgcc gctttcgccc tcgatgagca ccgtgaagtc ggtcgccgcg acgcgctcga
     1861 cctgctcacg cagtgccccc atgacgagcg tggatccaat gagctgcccc gggctcggcc
     1921 tgggcttgtt tccaaacaga gcccggcctc gcgcgacctc gaggaccagg ccgccaagat
     1981 gagcgagcgt gatgagcgct tcgaaatccg gatcgtcgag cggttttgcg ggatcgcagg
     2041 aggcttccag caccgcctgt accctcgggt ccgccgtcgg tacgccgagg acgatggagt
     2101 cggccgtgcg tgtgggcgtc acgagccttg cctggtagcg aacccgtact tcgcgcaatc
     2161 gaacggcacg caaatcgaga agctggcgga cctcctgctc gaaagtggcc tcgaggtcct
     2221 tgtcggtggt gttgcggacg agtgccgccc ccagggcttc gagcacctgc cgcaggctcg
     2281 cgatgctgac gtaggcccgc ggacacggga ctccatccct ctgcacggct ccgaatgtct
     2341 ctcccacgcc cacctccgtg agcggcaaac caagctcgat gccacgtcac ggccagcggc
     2401 gtaagctgtt cgtgccaaac gctttggagg accgccgtat cctatttcgt gcacgagccg
     2461 ggtaagccgg accctcggcc gtttcatcgc ggctcacgct catgggaaag cgctacccac
     2521 gactgcaagt ccttccaagt ccgcccgata aatggattaa ccctgggact cgcttgtgtt
     2581 ggtgtggttg gctacgtcgt ttctggctgc aaccggcggg gttctcaccc ggggctcgtg
     2641 ccgaaggccg caacacaagc gagctgtgga gtagaggtgt cagagaaacc ggtcgaagtg
     2701 tcggtcgtca tcccctgcct gaacgaggcg gacacgctgg cgacgtgcat ccgaaaggcc
     2761 agcgacggga tggcgcgcgc cggaatcaac ggtgagatcg tcgtggccga caatggcagc
     2821 acggacgact cccccgcaat cgcccgggcc gcgggcgccc ggctggtccc ggtggccgaa
     2881 cgaggctacg gcgccgcgct catgggcggc atcgacgccg cgcgcggccg cttcatcgtc
     2941 atgggcgacg ccgacgacag ctacgacttc ggcgaggtgc ccgcctttgt cgagcgcctg
     3001 agggaagggt tcgacctcgt ccagggatgc cgtctgccgt ccggtggagg acgtgtcctc
     3061 ccgggggcga tgccgcccct gcaccgatgg ttcggcaacc cgatgttctc ccgggtcgcg
     3121 cgcgcctggt tccgcgcgcc ggtccacgac atctactgcg gcctccgcgg tttcaccaag
     3181 gcgctctacg agcgtctcga tcagcgttgc acggggatgg agtttgccac cgagatgatc
     3241 gtgaaggcca gcctcaacgg ggcgcggatc agcgaggtgc cgatcacgct gcacccggac
     3301 ggccgacgga cccatgcgcc ccatctcaag acgttccgtg acgggtggcg cacgctgcgg
     3361 ttcttcctca tgtgcacgcc gcgccggctg ttcctgatgc cgggactcgt cctgctggtg
     3421 ctcgggctca tcggctacac ggtcgcgatg ccgggcgtca cgctcggcgg gctgaccttc
     3481 gacgcccaca cgctgctatt cgcgagcctc gcggtgatct gcggctatca gagcatgctc
     3541 ttctcgctca tcgccaagac gttcgccgcc tcggaaggcc tgcttcccgc ggatcggcgc
     3601 gtggagcgat tcgcctcgat cctgccgctc gagcggagcc ttctcgccgg cctgggcacg
     3661 gtagccatcg gcggcgtgat gctcgcggcc gcactgaacg aatggcgcgt cctgaacttc
     3721 ggccgcctcg actacgcgca cacgatgcgc gtcgtcgttc ccggagtcac gctctcggtg
     3781 ctcggatacc agacgatcct ctcgtccttt ttcctcagtc tcctggggat gcggcggcgc
     3841 tgatgtcgca attcgacgcg tacgcggcgg actacgacgc ggcgctcaac cgcgggctgt
     3901 cgctcagcgg cgagtcgagg gagtacttcg ccacggagcg ggtgaaatgg gtgtcgcggt
     3961 gccttgccga attgagcgcg cgtcccac
//
LOCUS       DBNGSH010001032         1570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1682691, whole genome shotgun sequence.
ACCESSION   DBNGSH010001032 DBNGSH010000000
VERSION     DBNGSH010001032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1682691"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="AC16CY_23465"
     CDS             complement(<1..434)
                     /locus_tag="AC16CY_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356831.1"
                     /translation="MIRVIAAVLLLSAPAFAQEWTEFVSKEDRFSANFPGPPQVRETT
                     YTSQFGADLPARVYSASLGPSRFSLTVVDYRPIEKILTEKSRSCPAGAETCLGGSNPE
                     SSTGAGYWKADVAGAIVYATFQFMQRDATVTHLVWSNIDLVEG"
     gene            complement(526..861)
                     /locus_tag="AC16CY_23470"
     CDS             complement(526..861)
                     /locus_tag="AC16CY_23470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356832.1"
                     /translation="MRIKIHNGTVSHGEQSLCGSCRHATITRGRTLDEEIVRCELRPM
                     STVFIRFKVTECSAYMDARLPSYAQLLEQAWILRPGKGRRPAGFVRSSELSEDELCEV
                     MTDLPEFGV"
     gene            1051..>1570
                     /locus_tag="AC16CY_23475"
     CDS             1051..>1570
                     /locus_tag="AC16CY_23475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3356833.1"
                     /translation="MWNRLQRPWQLGLLALGTAAVAAAQDATPQVPAFDVVSIRQFVD
                     GGPRRVEWTPGRFVALGAPLPGLVNMAYGGGAPVRLEGGDALRGMAWDITATFDPAFT
                     PTLEQRAAMLRAVLEDRFRLVLRRESRETDVYALLLVRPDGQPGPSLQPSELPCTSPP
                     RASFSVGRLPPDG"
BASE COUNT          238 a          535 c          508 g          289 t
ORIGIN      
        1 ccttcgacca ggtcgatgtt gctccagacg aggtgcgtca ccgtggcgtc gcgctgcatg
       61 aactgaaacg tcgcgtagac gatggcgccg gcgacgtcgg ccttccagta tcccgcgccg
      121 gtggagctct ccggattgct cccgcccagg caggtctccg cgccggcggg acacgacctg
      181 gatttctcgg tgaggatctt ctcgatcggg cggtagtcca ccacggtcag cgagaaccgg
      241 ctgggtccga gcgacgcgct gtacacgcgc gcggggagat cggcgccgaa ctgcgacgtg
      301 taggtggttt cacgtacttg gggtggaccc ggaaagttgg cgctgaaccg atcttccttg
      361 ctgacgaact cggtccactc ctgggcgaag gccggcgctg acagcaagag cactgcggca
      421 atgactcgaa tcacgccaac tcccgatttc caactcccac gagaagttcg ggcgttgatg
      481 ttactcctcc tgaacgcgag ggcgcgagct ggagctccgc cgggcttaga ccccgaactc
      541 cggcaggtcc gtcatgacct cgcagagctc gtcttctgac agctcgctgc tccgcacgaa
      601 gcccgccgga cgtcgccctt tgcccggcct gaggatccac gcctgctcga gcagctgcgc
      661 gtagctcggg agtcgcgcat ccatgtacgc cgagcactca gtcaccttga acctgatgaa
      721 cacagtgctc atcggccgca gctcgcagcg gacgatctcc tcgtccaacg tacgcccgcg
      781 tgtgatcgtc gcgtgacggc agctgccgca gaggctctgt tcgccgtgtg acaccgtgcc
      841 gttgtggatt ttgattcgca tttcgacgac gaatggtgca gcttgaatgc cagctccgcc
      901 acccgcctct ttttcgggcg gatcgcgcgg gcagggcgac ggcatcgctg ccgcagtttg
      961 gattcctgca accacgtttc ggccggatcg atggcacaaa ctgccgtccg gttgcggagc
     1021 cgatcctgga cactggtgtc aaatcccaac gtgtggaacc gtctccagcg tccctggcag
     1081 ctcggccttc tagctctcgg caccgccgcc gtggccgcgg cgcaggatgc gacgccacag
     1141 gttcccgcgt tcgacgtggt ctcgatcagg cagtttgtcg acggcggtcc ccgccgtgtc
     1201 gagtggacgc cgggccgctt tgtcgcgctg ggcgccccgc tccccgggct cgtgaacatg
     1261 gcctacggcg gcggcgcgcc cgtacgcctc gaaggcggtg acgcgctgcg agggatggcg
     1321 tgggacatca cggcgacctt cgatccggcg ttcaccccca cgctcgaaca gcgcgcagcg
     1381 atgctgaggg ccgtgctcga ggatcgattc cgcctcgtgc tgcggcgcga atcgcgcgaa
     1441 acagacgtct atgcgctgtt gctcgtacgg cccgacggcc agcccggccc atcgctgcag
     1501 ccctcggagc tgccgtgcac gtcgccgccg cgggcttcct tctcggtggg caggcttcct
     1561 cctgacgggc
//
LOCUS       DBNGSH010001033         3450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1853638, whole genome shotgun sequence.
ACCESSION   DBNGSH010001033 DBNGSH010000000
VERSION     DBNGSH010001033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1853638"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="AC16CY_23480"
     CDS             complement(<1..620)
                     /locus_tag="AC16CY_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356834.1"
                     /translation="MRLAALIVGCLLCSQPAHGQEPAAARPATIRVFIDCTNTPCDEN
                     YFRTEMTFVEHVRDRQNADVHILLTSQPTGGGGREITFDFFGQGRFMGRDDRLTERFL
                     VAVSQDEIRSGMVRVMSLGLVSYLLETEAISKLRLTTEPVDPAKTAAALVDDPWNRWS
                     LRANVNGNSSGERRSKSFSFNTNFNANRTTDETKVNLNARLNYRESR"
     gene            714..1151
                     /locus_tag="AC16CY_23485"
     CDS             714..1151
                     /locus_tag="AC16CY_23485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356835.1"
                     /translation="MRRFLLVTSASIALVGVTLMAQAKMSTEEYAKLMKANAGAFGPM
                     NKAIASGGYADAKTQLATLRTNYTALQGFWAGVKKDDAVAIVKDGLTRLDALDKMLSA
                     ATVDQAAAQAAAKEFQGATCGACHKAYREGDPQSGWRFKAGVL"
     gene            1163..1897
                     /locus_tag="AC16CY_23490"
     CDS             1163..1897
                     /locus_tag="AC16CY_23490"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3356836.1"
                     /translation="MRFRVVRERRIARSASLLLLVWAAGALLTAQAPARTVWDGVYTD
                     AQAERATATFNQSCARCHTLTADGTRPLSGDKFWEGYTQRTVADLLTYVRTNMPNGSG
                     GSLPAASYNDLVALILKSNGLPAGNTEITPETVADVQIIPKGGAGELPANTLVRVVGC
                     LAPRSGGDWVLTNGTAPVRIDKTGPVPEDATRPLGDRTMALKFVLTRLDSFVGQRMSV
                     SGILIGAGGAEGINVTTVNRVAATCP"
     gene            2048..>3450
                     /locus_tag="AC16CY_23495"
     CDS             2048..>3450
                     /locus_tag="AC16CY_23495"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3356837.1"
                     /translation="MRTARNSGLLIALLTFVALFPGGAGRAAADSPIVEAARRGDEAA
                     VRALIAKKANVNVRGNDGSSALLWATYHDDVELVRALLAAGAEVNAPNHYGITPLLQA
                     SRTGDAAVIDALLKAGADPRLNHPQGETPLMAASKTGRVNAVKLLLETGANVNAADTY
                     QQQTALMWAAADGHVEVVQALLDARADPNRKAAVTGLEERRHADHPTGGFTALMFAVR
                     NGHEQVARALVKGGADMKLTNGDGVTATIIAIVNDRLDLAGTLVDLGADPNDGSLYFA
                     VDMHDATTDMRARDGSRLRADHANTMTALDLVKKLLDKGADPNKGFQGQLHSLTLCCG
                     EDINSSPFYRAAVAADVEVLKLMIAKGAKVEWTPPEIKREGRGGGPGRGGNTGRTPMM
                     VAMTGGRGAAFAAGPGFERLSPPPFREASNREPLEAIKVLLSAGANPNAKSGDGTPLL
                     HQAVQARQVPIIRALAGA"
BASE COUNT          583 a         1136 c         1187 g          544 t
ORIGIN      
        1 cggctctccc ggtaattcag ccgcgcgttc agattgacct tcgtttcgtc ggtcgtgcgg
       61 ttcgcgttga agttggtgtt gaaggagaac gatttcgacc tccgctcgcc gctcgagttg
      121 ccgttcacat tggccctcag gctccatcga ttccaggggt cgtcgacgag cgcggcggcg
      181 gtcttcgccg gatcgaccgg ctcggtcgtg aggcgaagct tgctgatcgc ctccgtctcc
      241 agcaggtacg acacgaggcc cagcgacatg acgcggacca tcccgctccg gatctcgtcc
      301 tgcgacaccg cgacgagaaa ccgctcggtc aaccggtcgt ctcgtcccat gaagcggccc
      361 tggccgaaga agtcgaacgt gatctcgcgg ccgccgcccc ccgtcggctg gctcgtcagc
      421 aggatgtgga cgtcggcgtt ctggcgatcg cggacgtgct cgacgaacgt catttccgtg
      481 cggaagtagt tctcgtcgca gggggtgttc gtgcagtcga tgaagacgcg aatagtcgcc
      541 ggccgggccg ccgccggctc ctgtccatgg gccggctggc tgcagaggag gcatccgacg
      601 atgagggcgg ccagacgcaa gggattaatg gtatccgcat tgaatccgcg ccgcccgtgg
      661 gatagcattc cgactccccc acacggaaac ggtctctcaa ggagggcagc tctatgcggc
      721 gattccttct cgttacctcg gcgtcgatcg cgctcgtcgg ggtcaccctg atggcgcagg
      781 cgaagatgtc gaccgaagag tacgcaaagc tcatgaaggc gaacgcggga gccttcgggc
      841 cgatgaacaa ggccatcgcg tctggcgggt acgcggacgc caaaacgcag ttggcgactc
      901 tgcgaacgaa ctacaccgcg ctccagggct tctgggcggg cgtgaagaaa gacgacgccg
      961 tcgccatcgt caaggacggc ctgacgcgcc tcgacgcgct cgacaagatg ctgtcggcgg
     1021 cgacggtcga tcaggcggcc gcgcaggccg cggccaaaga gttccagggc gcaacctgcg
     1081 gcgcgtgcca caaggcgtat cgtgagggcg acccccagtc gggatggcgt ttcaaggccg
     1141 gcgtccttta atcgattagt cgatgcggtt tcgtgtggtg agggagaggc ggatcgcaag
     1201 atccgcctcg ttgctgctgc tggtgtgggc agccggcgcg ctgctgactg cgcaggcgcc
     1261 ggcgcgcacg gtctgggacg gcgtctacac cgacgcgcag gcggagcgcg ccacggcgac
     1321 gttcaatcag agctgcgccc ggtgtcacac gctcaccgcc gacggcacgc gacccctttc
     1381 gggcgacaag ttctgggagg gctacacgca acgaacggtc gccgatctgc tcacctatgt
     1441 ccgaaccaac atgcccaatg gcagcggcgg ctcgctgccg gccgccagct acaacgacct
     1501 cgtcgcgctg atcctgaagt cgaacggctt gcccgccggc aataccgaga tcacgcctga
     1561 aacggtggcc gacgttcaga ttatccccaa aggtggcgcc ggagagctgc cggccaacac
     1621 gctcgtgcgc gtcgtcggct gcctcgcgcc gcggtccggc ggcgactggg tcctgaccaa
     1681 cggcacggcc ccggtccgca tcgacaagac agggcccgtt cccgaggacg ccacccgtcc
     1741 tctcggcgat cgaacgatgg cgctgaagtt cgtcctcacc cggctcgatt cctttgtcgg
     1801 ccagcgcatg tcggtcagcg gcatcctcat cggggcgggc ggcgccgaag gcatcaacgt
     1861 gacgaccgtc aaccgggtcg ctgcaacctg cccctgattc gcctctatct gatactttcc
     1921 agacgacttt cgttataggc gcgggttata ggtctgcccg gccgacggac cgtctacggg
     1981 tgtaaaatcg gccttgtcac gacccccgtc gcgagcgggt attcgtgtag aaagagtgga
     2041 gccgtcgatg cgcaccgcac gaaattccgg actgttgatc gcgctgttga cgttcgtcgc
     2101 gctgttcccg ggcggggccg ggcgcgccgc ggcggacagc cccatcgtcg aggcggccag
     2161 gcgaggtgac gaggcggccg ttcgcgcgct gattgcgaaa aaggcgaacg tcaacgtccg
     2221 cggcaacgac ggctcgtcgg cgcttctctg ggcgacctac cacgacgacg tcgaattggt
     2281 gcgcgcgctg cttgccgcgg gcgcggaggt gaatgcgccg aatcattacg gcatcacccc
     2341 gctgcttcag gccagccgca cgggcgatgc cgcggtcatc gacgcactgc tcaaggcagg
     2401 cgcggatcct cggctgaacc accctcaggg cgagacgccg ctgatggcgg cttcgaagac
     2461 ggggcgcgtg aacgcggtga agctgctgct cgagacgggc gccaacgtga acgccgcgga
     2521 tacctatcag cagcagacgg cgctcatgtg ggccgcagcc gatggccacg tggaggtggt
     2581 tcaggcgctg ctcgacgcgc gggcggatcc gaatcgcaag gcggcggtca ccggcctcga
     2641 ggagcgccgc cacgccgacc acccgacggg cggattcacg gcgttgatgt tcgccgtccg
     2701 caacgggcac gaacaggtcg cgcgcgcact cgtcaagggc ggcgcggaca tgaagctgac
     2761 caacggcgac ggcgtgacgg cgacgatcat cgccatcgtg aacgatcggc tcgacctggc
     2821 gggcacgctg gtcgatctcg gcgcggaccc gaacgatgga tcgttgtact tcgccgttga
     2881 catgcacgac gccacgaccg acatgcgcgc gcgcgacggg tcgcgcctgc gcgccgatca
     2941 cgccaacacg atgacggcgc tcgacctcgt caagaagctg ctcgacaagg gcgcggatcc
     3001 gaacaagggc ttccaaggcc agctccactc gctgacgctg tgctgcggcg aggacatcaa
     3061 ctcgtcgccg ttctatcgcg cggccgtggc ggctgacgtc gaggtgctga agctgatgat
     3121 cgcgaagggc gcgaaggtcg agtggacgcc gcccgagatc aagcgcgaag gccgcggcgg
     3181 cggccccggg cgcggcggca ataccggccg gacaccgatg atggtcgcca tgacgggcgg
     3241 ccgcggcgcc gcgttcgcgg cgggtcccgg gttcgagcgc ttgagcccgc cgcctttccg
     3301 cgaggcgtcg aatcgcgagc cgctcgaggc catcaaggtg ctcctctccg cgggggcgaa
     3361 cccgaacgcc aagtcgggcg acggcacgcc gctgctgcac caggcggtgc aggcgcggca
     3421 ggtgccgatc attcgcgcgc tggcgggagc
//
LOCUS       DBNGSH010001034         2012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1684320, whole genome shotgun sequence.
ACCESSION   DBNGSH010001034 DBNGSH010000000
VERSION     DBNGSH010001034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1684320"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..323
                     /locus_tag="AC16CY_23500"
     CDS             36..323
                     /locus_tag="AC16CY_23500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356838.1"
                     /translation="MPFLVGCLALSAPRFAIALVVLFSDYIGRAYETALWPFIGFLFM
                     PLTTLAYAWAINSRGSVEGVHLIVVVIAVLVDLGLAGGSASERRRKRKRER"
     gene            complement(522..941)
                     /locus_tag="AC16CY_23505"
     CDS             complement(522..941)
                     /locus_tag="AC16CY_23505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356839.1"
                     /translation="MKPLQWWLRIVGVLYVFEGGGLALAALFDPNEFAAIWSSTEAGT
                     LDALAVRGIRIAGLPGVLTWALLGAMMLIFSRVPARARMLVVTVAAWELLVWLPVDLV
                     AMFDGFGVARAATLITIHVVIGVSGIVLLRRQRGDSG"
     gene            complement(938..1276)
                     /locus_tag="AC16CY_23510"
     CDS             complement(938..1276)
                     /locus_tag="AC16CY_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR3356840.1"
                     /translation="MDFDVVKQIARTLPDVEEHTSPRGTGFTVRGKLLACEAVHTSAE
                     PGSLMVRIGSVEREFLLAAEPDAYYLTDHYRGYPAVLVRLSKISRKALRERLEGAVKF
                     VGARKPRARR"
     gene            complement(1390..1908)
                     /locus_tag="AC16CY_23515"
     CDS             complement(1390..1908)
                     /locus_tag="AC16CY_23515"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3356841.1"
                     /translation="MKMTRMMAVAAVAGAIAWASIGIGAQDNTMREARDRDEIEALMW
                     RYTRALDTANAEAYASAYTETGQFSAGGANPTKGRAALTKMIADIKARQTAAAAKGEP
                     QAPMYHMTLNSQISFIDKDHARIDAYYQTVFGAGGQNTPPRLAAAGRSVDLLVRQNGK
                     WLIESRDVAPRD"
BASE COUNT          338 a          694 c          629 g          351 t
ORIGIN      
        1 atcgcctacc gccgctgatc agctagactc ggcgcatgcc tttcctcgtc gggtgccttg
       61 cgctgtcggc gccacgattc gccatcgcgc tcgtggtgct cttcagcgac tatatcggcc
      121 gcgcctacga gacggccctc tggccgttca tcggattcct gttcatgccg ttgacgacgc
      181 tggcctacgc gtgggcgatc aacagccgcg ggtccgtgga gggcgttcac ctgatcgtag
      241 tcgtcatagc agtcctggtg gatctgggtc ttgccggcgg ttcagcctcg gagcggaggc
      301 gcaagcgcaa gcgtgagcgc taaacagtat ccggagccgg gttcggttct cggttcgtgg
      361 ttcctacggt ccggctgaag ccggacacta cgatcgattg gtgtgcgcgg cgtaagggtg
      421 tccgacttca gccggacttg cgaacgtgaa cgcgaaccga gaagtgagaa gcgagaagtg
      481 tgaacacacg atctctgagg gtcgactcaa gtgatttgac cttaaccgct atctccgcgc
      541 tgccgtcgaa gcagcacgat ccccgagacg ccgatgacga cgtggatggt gatcagcgtg
      601 gcggcccgcg ccacgccgaa cccgtcgaac atggccacga gatcgacggg cagccacacc
      661 agcagctccc acgccgcgac ggtgacgacg agcatgcggg ctcgcgccgg gacgcgcgag
      721 aagatcagca tcatggcgcc gagcagcgcc catgtgagaa cgcccggcag tcccgcgatc
      781 cgaatccccc gaacggccag tgcgtcgagc gtgccggcct cagtcgagga ccagatggcc
      841 gcgaactcgt tcggatcgaa gagcgccgcg agcgccagcc cgccgccttc gaacacgtac
      901 agcacgccga cgatgcgcag ccaccactgc agcggcttca tcgccgggct cgcggcttcc
      961 tggcgcccac gaacttcacc gccccctcga gccgctcgcg aagggcctta cggctgatct
     1021 tcgaaagccg caccaggacg gccggatagc cgcgatagtg atcggtcaga tagtacgcgt
     1081 ccggctccgc cgcgagcagg aactcgcgct cgaccgatcc gattcgaacc atgagcgagc
     1141 caggctccgc cgacgtgtga accgcctcgc acgccaggag cttgccgcgg actgtgaacc
     1201 ccgtgcctcg gggcgaggtg tgctcctcga cgtccggcag cgttcgcgcg atctgcttga
     1261 caacgtcgaa gtccatgccg cgtcgtgttg ccactgttct agggctcggg gctcagggac
     1321 caatgaccaa gtacggaccc aggaccaagt acggacccaa ggaccaagaa caaagcacca
     1381 agaaccaagt cagtcgcgag gcgcgacgtc gcggctctcg atcagccact tgccgttctg
     1441 cctgaccagc aggtccacgc tgcggccggc cgcagccagg cgtggcggcg tgttctggcc
     1501 gcctgcgccg aacaccgtct ggtaataggc gtcgatgcgc gcgtggtcct tgtcgatgaa
     1561 cgagatctgc gagttcaggg tcatgtgata catcggcgcc tgtggctcgc ccttcgcagc
     1621 ggccgcggtc tgccgcgcct tgatgtcggc aatcatcttc gtgagcgcgg cgcgcccctt
     1681 ggtcgggttc gcgccgccgg cgctgaactg gccggtctcg gtgtaggccg acgcgtacgc
     1741 ttcggcattc gccgtgtcga gcgcgcgcgt gtagcgccac atcaacgctt cgatctcgtc
     1801 gcgatcgcgg gcttcgcgca tcgtgttgtc ctgcgcgccg atcccaatgg acgcccacgc
     1861 gatcgcgccc gcgaccgccg ccactgccat catcctcgtc atcttcatca caggctcctc
     1921 acagtgaatg cctcgactgc ccgtcggcct cgcgggctga ttctaccgcc atctcagatc
     1981 ccgtcttctc ttgccccctg ccccctgccc cc
//
LOCUS       DBNGSH010001035         4463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2365168, whole genome shotgun sequence.
ACCESSION   DBNGSH010001035 DBNGSH010000000
VERSION     DBNGSH010001035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2365168"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="AC16CY_23520"
     CDS             <1..1583
                     /locus_tag="AC16CY_23520"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356842.1"
                     /translation="SRTVIAASLFAALVATALPAAQEPADPPSIAPAGSGLLAVPLPR
                     IDELEPAVSDQIREQYRSFIDVTAGKPATRALADAYGSMGQLLHAYEFLDGAEPAYRN
                     AARLAPGDGRWPHLLGFLCQQTGRLDEAAGFYREASRLQPGDRAAAAQLGAVYLGLNR
                     LAEAGQQFRGITEIFPAVARQGLGEVALREGKFADAIDHLAAALQRAPQASSIHYSLA
                     MAYRGLGRMDQARSHLRQRGPGELRPADPIVDGLQALVRGERAHVIQGRRAYEAGRFQ
                     EAADAFSKAVAASPASVAARVNLGMALAQTGDAVGARGHLETALRLDADNVTAHAGLG
                     MMLARLGRDDEAVDHLRAAFDRTPEDAGIRTELVGSLLRLRRNDDAIEVLAAARSIDP
                     DDEGLLVNLSILLADRERYREAVTLLDEAHARFPERAATATTLARLLASSPDLSVRDG
                     RRALAIAMDVYTREPTPVRRETVAMSLAELGRCDEAAEWIRRAVTEAAQLKDTMESAR
                     LRGEAAKYETASCRPPGR"
     gene            complement(1696..>4463)
                     /locus_tag="AC16CY_23525"
     CDS             complement(1696..>4463)
                     /locus_tag="AC16CY_23525"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3356843.1"
                     /translation="VVTVNADMALGTLEETVTVTGATPTVDLQSTTRQVSITQEIVSA
                     IPSSRTPFSLGVLIAGVRQDFGGRDVGGAVVAEVASLVANGGRTNDQRMMVNGVALSS
                     GIAGGWGGGAVPNATGTAEFAIDVSGVDAQAATGGVRVNFIPRDGGNRFSGTIFGSYS
                     RGNFAGDNYTDSDVEDRGLVSPNKIKGNGDFNPGAGGPIVRDKVWFFLSGRYLFADNY
                     QASVFFNANANDPNQYRYANTGEQAVLHQDQQIYQARFTWQASARDKFGATYDQENYC
                     GCPFGLTGAGGALISPEAATDRRFPLQRFVTADWTSPVTNRLLLEASGIHRVERWGNM
                     HLQTGKGENIESLTPGITSVTDNPSLATGGNFTYRSAAQFNNSWNWNIHYRAAVSYIS
                     GGHNIKVGFNNAYLHHENTTYTDPTTPYSYNFVSGNPSQIVYRIAPRTVAVNVDYDLG
                     VFAQDRWTVGRWTLQGGVRFDTFKNHFPAQSIAPTALAPTLNVSFPKTDSLNWKDVTP
                     KMGATYDLFGDGRTALKVTLNKYLEGLGTTGFGPAQVSDAPNPINRLNLGGPGVTFRN
                     WSDTTGLGINNDFIPQCDLLNFAAHQECGAVVNGATFGNVTGTTVYDPDLMTGWGKRG
                     YNWEFTAGVQHELMPRVSLSVQYARRTYGNIRIMDDTAVTSAEYQRFTMTVPTDSRLP
                     NSGGTLTAFDLTPAGGTTAARYLVTLAENYGHQTEHFDGVNISVSARLQNGLMLQAGL
                     GPGRQVTNDCEVVDDLPEMLHTLAGNPTRNSLFAARPLDRCEQNNGWRTGVSGLATYT
                     IPKIDVQVSGTFQNQPGAQLDANALTAASSTTLGRGFTNAPGGRFFNIVPAGEVFIER
                     LNQIDLRVSKLFRIGGTRTSLNFDFYNMTNSNSVLTENPTFGAAWRTPQSILLPRLFK
                     MSAQFDF"
BASE COUNT          715 a         1456 c         1504 g          788 t
ORIGIN      
        1 tcagccggac cgtgatcgcc gcgtcgttgt tcgccgctct cgttgcgacc gcgctgccag
       61 cggcacagga accggcggac cctccatcga tcgcgcccgc cggatccgga ctgcttgcgg
      121 ttccgctccc acgaatcgac gaactcgagc cggcggtgtc ggaccagatt cgcgaacagt
      181 acagatcgtt catcgacgtg acggcgggga agccggcgac gagagcgctg gccgacgcgt
      241 acgggtcgat gggccagctc cttcacgcgt acgagttcct cgatggggcc gagccggctt
      301 acagaaacgc cgcgcgcctg gctcccggcg acggccgctg gccccatctg ctcggcttcc
      361 tgtgccagca gaccggacgg ctcgatgagg cggcgggctt ctatcgggag gcgagccggc
      421 tgcagcccgg cgatcgggcc gcggccgcac agcttggggc cgtgtacctc gggctcaacc
      481 gactggccga ggccgggcag caatttcgcg gcatcaccga gatctttccc gccgtcgcgc
      541 gccagggtct gggcgaggtc gcgctccgtg aaggcaagtt cgcggatgct atcgaccatc
      601 tcgccgccgc gctccagcgg gcgccgcagg cgtcgtccat tcactattcg ctggcgatgg
      661 cctaccgcgg tctgggccgc atggaccagg cgcgatcgca cctgcgacag agaggcccgg
      721 gcgagcttcg gcctgccgat ccgatcgtgg acggccttca ggctctcgtg cgcggcgagc
      781 gggcccacgt catccagggg aggcgagcct acgaagccgg ccggtttcag gaggccgccg
      841 atgcattcag caaggcggtc gccgcgtcgc cggccagcgt cgcagcccgg gtgaacctcg
      901 gcatggcact ggcacagacc ggcgatgccg tcggggcgag ggggcacctc gagacggcac
      961 tcaggctcga tgctgacaac gtcacggcgc atgcgggcct cggcatgatg ctcgccaggc
     1021 tggggcgcga tgacgaggct gtcgaccacc tacgcgcggc cttcgaccgg acgcctgaag
     1081 acgccggcat caggaccgag ctcgtcggct cgctgttgag acttcgccga aacgacgacg
     1141 cgatcgaggt gctcgccgca gcgcgatcga tcgatcccga cgacgagggt ctcctcgtga
     1201 acctgtcgat cctgctggca gatcgagagc gctatcgtga ggccgtgacg ctgctggacg
     1261 aagcccacgc acggtttccc gaacgagccg ccacggcgac gacacttgcg cgcctgctgg
     1321 cctcgtcgcc cgatctctcc gtccgcgacg gacgccgggc gctcgcaatc gccatggacg
     1381 tgtatacgcg agaaccaaca cccgtacgcc gggagaccgt cgcgatgtca ctggccgagc
     1441 tgggacgatg tgacgaggcc gccgagtgga tacggcgcgc ggtcaccgag gccgcgcagc
     1501 tgaaggacac gatggagagc gcgcgtctca ggggcgaagc ggcgaagtac gagacggcct
     1561 cgtgcaggcc gccgggacga tagccatcac gaagttctcg ccaggaagat cacgaagacc
     1621 ctgccacgaa gtcacgaaga gaatctggct tcgtgttcat cgtggccgat gtcttcgcgt
     1681 ctccatggcc gcgcgtcaga aatcaaactg tgcgctcatc ttgaagagcc gggggagcag
     1741 gatcgactgc ggcgttcgcc acgcggcgcc gaacgtcggg ttctccgtca gcaccgagtt
     1801 ggagttcgtc atgttgtaga agtcgaagtt gaggctcgtc cgcgtcccgc cgatccggaa
     1861 gagcttcgac acgcggaggt cgatctgatt caaccgctcg atgaacacct cacccgccgg
     1921 aacgatattg aagaaccggc cgccgggcgc gttggtaaac ccgcgcccga gcgtggttga
     1981 cgaggctgcg gtgagggcgt ttgcgtccag ctgcgccccc ggctggttct ggaaggtgcc
     2041 gctgacctgg acgtcgatct tcgggatcgt gtacgtggcc agtccggaga cccccgtccg
     2101 ccacccgttg ttctgctcgc agcgatccag cggccgtgcc gcgaagaggg agttgcgcgt
     2161 gggattgcct gcgagggtgt ggagcatttc gggcagatcg tccacgacct cgcagtcgtt
     2221 ggtgacctgg cgacccgggc ccaagccggc ctgcaacatc aggccgttct gcagccgcgc
     2281 cgaaaccgaa atgttcacgc cgtcgaagtg ctctgtctgg tgtccgtagt tctcggcgag
     2341 cgtgacgagg tacctcgccg ccgttgtccc gcccgccggc gtcagatcga acgcggtgag
     2401 cgtccccccg ctgttcggca accgggaatc agtcggcacc gtcatggtga atcgctgata
     2461 ctcggctgac gtcaccgccg tgtcatccat gatcctgatg ttgccgtagg ttcttcgcgc
     2521 gtactgcacg ctgagcgaca cgcgaggcat gagctcgtgc tgaacacccg cggtgaactc
     2581 ccagttgtag ccgcgcttgc cccacccggt catcaggtcc ggatcgtaga cggtggtgcc
     2641 ggtcacgttg ccgaacgtgg cgccgttgac gaccgcgccg cactcctggt gggcggcgaa
     2701 gttcagcagg tcgcattgcg ggatgaagtc gttgttgatc ccgaggccgg tcgtatcgct
     2761 ccagtttcgg aacgtcacac ccggtccccc caggttcagg cggttgatgg ggttcggcgc
     2821 gtcggacacc tgtgcagggc cgaagccggt ggtgcccagg ccctccaggt acttgttcag
     2881 cgtcaccttc agcgccgtgc gcccgtcgcc gaacaggtcg taggtcgcgc ccatcttcgg
     2941 cgtgacgtct ttccagttca ggctgtctgt cttcgggaag ctgacgttca gcgtgggcgc
     3001 cagcgccgtg ggcgcgatcg actgcgccgg gaagtggttc ttgaacgtat cgaagcggac
     3061 tcctccctgg agagtccatc ggccgaccgt ccagcggtcc tgcgcaaaca ccccgaggtc
     3121 gtagtcgaca ttcaccgcga ccgtgcgagg cgcgattcga tagacgatct ggctcgggtt
     3181 gccgctgacg aagttgtagc tgtacggcgt cgtgggatcc gtatacgtcg tgttctcgtg
     3241 atgcaggtag gcgttgttga agcccacctt gatgttgtgg ccccctgaga tatacgagac
     3301 cgccgcccga tagtggatgt tccagttcca ggagttgttg aactgtgcgg ccgatcggta
     3361 cgtgaagttt cctcccgtcg cgagcgacgg gttgtcggtc accgaggtga tgcccggcgt
     3421 cagcgactcg atattctccc ccttgcccgt ctgcaggtgc atgttgcccc agcgctccac
     3481 gcggtggatg ccgctcgcct cgagcagcag gcggttcgtg accggagagg tccagtcggc
     3541 cgtgacgaac cgctggaggg ggaacctccg gtcggtggcc gcctcgggcg aaatcagcgc
     3601 gccgccggcg cccgtcagcc cgaacggaca gccgcagtag ttttcctgat cgtaggtggc
     3661 gccaaacttg tccctggcgc tcgcctgcca cgtgaatcgg gcctggtaga tctgctgatc
     3721 ctggtgaagg acggcctgtt caccggtatt tgcgtaccgg tactgattcg ggtcgttggc
     3781 gttcgcgttg aagaacaccg acgcctggta gttgtccgca aaaaggtagc ggcccgagag
     3841 gaagaaccag actttgtccc ggacgatcgg gccgccggct cccggattga agtcgccgtt
     3901 gcccttgatc ttattcggag aaaccagccc gcggtcctcc acgtcgctgt ccgtgtagtt
     3961 gtcgccggcg aagttgcccc tcgagtagct gccgaagatc gtgcccgaga atcggttgcc
     4021 gccgtcgcgc gggatgaagt tgacgcgaac accgccggtg gccgcctggg cgtccacgcc
     4081 ggacacgtcg atggcaaact ccgctgtgcc ggtcgcgttt ggaaccgcgc cgcctcccca
     4141 gccgccggcg atcccggagc tgagcgccac gccattcacc atcatgcgct ggtcgttcgt
     4201 ccggccgccg ttggcgacga gcgacgccac ttcggcgacg acggcgccgc caacgtcgcg
     4261 gccgccaaag tcctgcctga cgccggcgat cagcacgccc agcgagaacg gggtgcgcga
     4321 gcttggaatc gccgagacga tctcctgggt gatcgagact tgccgcgtgg tgctctgcag
     4381 gtccaccgtg ggcgtagcgc cagtgacggt caccgtttcc tcgagcgttc caagcgccat
     4441 gtccgcgttg acggtcacga cag
//
LOCUS       DBNGSH010001036         1803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2195533, whole genome shotgun sequence.
ACCESSION   DBNGSH010001036 DBNGSH010000000
VERSION     DBNGSH010001036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2195533"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="AC16CY_23530"
     CDS             <1..1257
                     /locus_tag="AC16CY_23530"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3356844.1"
                     /translation="MGGWHPNQWAEHRHPTLKELDEAVPDRPVLLYERFTGPAVTNSL
                     GKKFFDAADAASPVHPEIKRVSVAENGAIAAAGFAGGGPAASALFHLRLAQTFDDKKR
                     STLDAMAYSASLGLTAHLDEVLFPTPGPLHPNQILSNLDQYRMYDSWLALHREGRTSI
                     RLQMNFLQNQNDPALPELKERLRNQFQFFGDDMLMTGSIGEWAAPLGSGAVWREAQRL
                     VAQARWRNENSVQNLAGLTQVVDAYEAVNKEFDITGLRWMVHHVPEVTADLLTRLRAM
                     GCGVQMGAFRWVTSTDPKVVVGPPFRTIVDHGIQVGIHGDGVHIAPLNPWQHIYFATT
                     GVNSFGARVNGDQQITRQEALRLFTRGNSWFLRMEDRIGSIEPGKLADLVVLDRDYFT
                     VPDVQLKQIRSLMTVVGGRIVHGSIG"
     gene            1271..>1803
                     /locus_tag="AC16CY_23535"
     CDS             1271..>1803
                     /locus_tag="AC16CY_23535"
                     /inference="COORDINATES: protein motif:HMM:NF017591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR3356845.1"
                     /translation="MSFSRRSFLHASGMAAFALPLEALRSRVEAGLPVAGEPHYGPLS
                     PVRDGTTGLPLLELPEGFRYTTFGWNGDPLDDGKRTPSAHDGMAAFDGGDGRIVLIRN
                     HEIGPGDPFGETAYDRNAGGGTTTLVFDPASERLVSARASLSGTIRNCAGGPTPWNTW
                     LSCEESLVGPRDRSPMPR"
BASE COUNT          306 a          629 c          591 g          277 t
ORIGIN      
        1 atgggaggct ggcacccgaa ccagtgggcc gagcatcggc acccgacgtt gaaggaactg
       61 gacgaagccg tgccggacag gccggtcctg ctgtacgagc gcttcaccgg cccggctgtg
      121 accaacagcc tcggcaagaa attcttcgac gccgccgatg cggcttctcc cgtgcatccc
      181 gagatcaagc gcgtgagcgt cgccgagaac ggcgccatcg cggccgcggg ttttgccggc
      241 ggcggaccgg ccgcaagcgc actcttccat cttcggctcg cacagacctt cgacgacaag
      301 aagcggagca cgctcgacgc gatggcgtac tcggcgagcc tgggactgac ggcgcacctg
      361 gacgaagtgc tcttccccac gcccggaccg ctccacccga accagatcct gtcgaacctc
      421 gatcagtacc gcatgtacga ttcgtggctc gcccttcacc gcgaagggcg gacgtccatc
      481 cgcctgcaga tgaacttcct gcagaaccag aatgatccgg cgctcccgga gctgaaggaa
      541 cgcctccgca accagttcca gttcttcggc gacgacatgc tgatgaccgg gtcgatcggc
      601 gagtgggccg cgccgctcgg gagcggcgcc gtctggcgcg aggcccagcg gctggtggcg
      661 caggcgagat ggcgcaacga gaacagcgtg cagaaccttg ccggcctgac gcaggtcgtc
      721 gacgcgtacg aagcggtcaa caaggagttc gacatcacgg gcctgcgctg gatggtgcac
      781 cacgtcccgg aggtcaccgc agacctcctt acgcgcctca gggcaatggg ctgcggcgtg
      841 cagatgggcg ccttccgctg ggtcacctcc accgacccga aggtcgtcgt cggtccgccg
      901 ttccgcacca tcgtcgatca cggcatccag gtcgggattc acggcgacgg cgtccacatc
      961 gccccgctca atccctggca gcacatctac ttcgcgacga ccggcgtcaa ctccttcggc
     1021 gcgcgcgtca acggcgacca gcagatcacg cgtcaggaag cgctccgcct cttcacgcgc
     1081 gggaacagct ggttcctgcg catggaagac cggatcggat cgatcgagcc cgggaagctc
     1141 gcggacctcg tcgtgctcga ccgcgactac ttcacggtgc cggacgtgca gctcaaacag
     1201 atccgctccc tgatgaccgt cgtgggcggt cgcatcgtgc acggctcgat cggctaaaat
     1261 accggcagac atgtccttca gccggcggtc gtttctgcat gccagcggga tggccgcctt
     1321 cgcgctccct ctcgaggctc ttcgctcacg tgtggaggcc gggctgccgg tggccggtga
     1381 gccccactac ggtccgctga gtcccgtaag ggacgggacc actggactac cccttctcga
     1441 gctcccggag ggcttccgct acacgacgtt cggatggaac ggcgatccgc tcgatgatgg
     1501 caagcgaacg ccgtcggcgc acgacgggat ggccgcgttc gacggcggcg acgggcgcat
     1561 cgtgctgatc cgcaaccacg agattgggcc cggggacccc tttggcgaga cggcctacga
     1621 tcgcaacgcg ggaggcggca ccacgacgct cgtgttcgac cccgcgtcgg agcgcctggt
     1681 gagcgcgcgc gccagcctct cgggcacgat ccgcaactgc gccggcggcc cgacgccgtg
     1741 gaacacgtgg ctgtcgtgcg aggagtcgct ggtcgggccc cgcgaccgca gtcccatgcc
     1801 gcg
//
LOCUS       DBNGSH010001037         1882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2196705, whole genome shotgun sequence.
ACCESSION   DBNGSH010001037 DBNGSH010000000
VERSION     DBNGSH010001037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2196705"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1882
                     /locus_tag="AC16CY_23540"
     CDS             <1..>1882
                     /locus_tag="AC16CY_23540"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3356846.1"
                     /translation="PALGRFFVPEEDRVPGRDAVIVLAHETWAEQFGSDPAVLGSRVK
                     VGGVDFTVIGVAPASFTGMHLVLPPHFYVPMAMTPSLAGESPRALEQRDARGLDVRGL
                     LKPGVSLEQARQESAVIAAGLEKAFPDTNRNIGTTVKNALDARLEERGPSAPSVFTLM
                     TLALVVLLVACANVAGLLLSRAPERQREIGLRLAIGGGRFRLVRQLLTESLLLAVGGG
                     LLGLAVGYGGVVLLHQLPIISDIGVRIRFDLDRRAITVGLTLAAASVILSSLVPAWRA
                     TRATDLTTTLRTGTARLERLSRLWGRNALVAIQVALSLVLLTVTAFLSRSFEAELNRP
                     GFRTERMLLAGFEPELASYDASRTDAFYRLLKERARNIPGVTSVGMTSILPLNQDNRE
                     SRRIVPEGFQLPQGTENVPILSSRIDEGYLETMAIPIVRGRGFQPNDTADSRLVVLVN
                     VAMANRYWPGQDPIGKRIQLLDREGGPWAEVVGATADNKYNWIGEPPTPWMYLPWQQE
                     PGPRATLLVAASRDSSGLAIPVRQIVREIDANMPISGMRTMEDYYHGNAVGIVTTLLR
                     VTGSMGLMGMAMAVVGLYGLVAYFVARRTREIGIRMAVGARASSILGLVLRHGLMLTA
                     LGV"
BASE COUNT          286 a          617 c          646 g          333 t
ORIGIN      
        1 gcccgcgctg gggcgcttct tcgtgccgga agaggaccgc gtgccggggc gcgatgccgt
       61 catcgtgctc gcgcacgaga cgtgggccga gcagttcgga tccgatccgg ccgttctcgg
      121 cagccgcgtg aaggtcggcg gcgttgattt cacggtgatc ggtgtcgcac cggccagctt
      181 cacgggcatg catctcgtgc tgcctccgca cttctacgtt ccgatggcca tgacgccttc
      241 gctcgcggga gagtctccac gcgccctcga gcagagggac gcgcgtggcc tcgacgtccg
      301 cggcctgctc aagcccggcg tttccctgga gcaggccagg caggagtcgg cggtcattgc
      361 cgccggcctc gaaaaggcgt ttcccgatac gaaccggaac atcgggacga cggtgaagaa
      421 cgctctcgac gcccggctcg aagagcgggg cccctccgcg ccgagcgtgt tcacgctgat
      481 gacgctcgcg ctggtcgtgc tcctggtggc ctgcgcgaac gtcgccggcc tcctgttgag
      541 ccgcgctccg gagcgacagc gcgagatcgg cctgcggctg gccatcggcg gcggcaggtt
      601 ccggctcgtc aggcagttgc tgaccgaaag ccttctgctc gccgtaggag gcggcctcct
      661 cggccttgcg gtcggctacg gcggggtggt gctgctgcac cagttgccca tcatcagcga
      721 catcggtgtc cgcatcaggt tcgacctgga tcgccgcgca attaccgtgg gtcttacgct
      781 ggccgcggca agcgtcatcc tgtcaagcct cgtccccgcg tggcgtgcga cacgcgcgac
      841 cgatctcacg acgacgctgc gaacggggac cgcgaggctc gagcggttgt cgcggctgtg
      901 gggccgcaac gcactcgtcg ccattcaggt ggctctgtcg ctcgtgctcc tcaccgtcac
      961 ggcatttctg tctcggagct tcgaagccga gttgaaccgc cctggctttc gaaccgagcg
     1021 catgctcctc gcgggcttcg agcccgagct cgcctcgtac gacgcgtcac gcaccgacgc
     1081 gttctaccgc ctgctcaagg agcgtgcccg taacatcccg ggcgtgacgt cggtcggcat
     1141 gacctcgatc ctgccgttga accaggacaa ccgggaatcg aggaggatcg tcccggaagg
     1201 gtttcagctg ccccagggga cagagaacgt accgattctc tcgtcccgca tcgacgaggg
     1261 ctatctcgag acgatggcca tcccgatcgt gaggggacgc ggctttcagc cgaacgatac
     1321 ggcggactcg cggctcgtgg tccttgtcaa cgtcgcgatg gcgaaccgct actggccggg
     1381 gcaggatcca atcggcaagc ggatccagct tctcgaccgt gaaggagggc cgtgggcgga
     1441 ggtcgtcggc gccacggcgg acaacaaata caactggata ggcgaaccac cgactccgtg
     1501 gatgtacctt ccgtggcagc aggaaccggg acctcgcgcc actctgctcg tggcggcctc
     1561 tcgcgactcg agcgggcttg ccattcctgt tcgccagatc gtccgcgaga tcgatgcgaa
     1621 catgccgatc tccggcatgc ggacgatgga ggactactat cacggaaacg ccgtcggcat
     1681 cgtgacgacg ctgctccgcg tcacgggcag catggggttg atggggatgg cgatggcggt
     1741 ggtgggactc tacggcctgg tcgcgtactt cgtcgcgcgg cgcacgcggg agatagggat
     1801 ccggatggcg gttggcgcca gggcgtcatc gatcctgggc ctcgtgctgc gccatgggct
     1861 catgctgacg gccctgggcg tg
//
LOCUS       DBNGSH010001038         2846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_158481, whole genome shotgun sequence.
ACCESSION   DBNGSH010001038 DBNGSH010000000
VERSION     DBNGSH010001038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_158481"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="AC16CY_23545"
     CDS             <1..715
                     /locus_tag="AC16CY_23545"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR3356847.1"
                     /translation="AAFDLGLAAGADGLEIDVHLSSDGVVVVCHDATLDRTTDASGPV
                     AARTAAELARVDAGFRYGSNGDHPFRGRGVGIPALRDVLRRYPDTPIIVEMKVDGREM
                     GLALAADVRGASAMDRVCAASSGCRALRAVREALPGLATSACRWDVRLALYRSWVGWP
                     VRQAAYGGFQVPEVAGATRIVSRDFIRHAHEGGFEVQVWTVDEEADMERLLAWGADGL
                     ITNRPAAAVAVRDRRSVRL"
     gene            730..1701
                     /locus_tag="AC16CY_23550"
     CDS             730..1701
                     /locus_tag="AC16CY_23550"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HIR3356848.1"
                     /translation="MASAMNPPTFEDVVAARERVYAHMRPSPLLNHPLLDEWVGCRVW
                     VKHENHNPTGSFKIRGGLNLVAQLSADERQRGVIAASTGNHGQSLALACRLHGVRCRI
                     VVPVGNNPDKNAALRAFGAELVEHGRDFDEAREYVEAVAGREGWRYVHSANEPHLIAG
                     VGTYAVEVFEAQPGIDRIFVPIGGGSGAAGCAIVRSGLGLPTQIIGVQAQQADAFTRS
                     WRGPERVTRDRVDTFAEGLATRTTFDLTFGLLKATTGLDEVVTLEEEELREGVTAAIQ
                     RTHNLAEGAGAATLAAARRAGLPRDATVVCVMSGGNADARTLRHALA"
     gene            1766..2200
                     /locus_tag="AC16CY_23555"
     CDS             1766..2200
                     /locus_tag="AC16CY_23555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356849.1"
                     /translation="MRRLLTYSTVVLVALAGTVAAQKATTPAELEGAMKRISAANGAV
                     AKAIKSGAFEDAKTQVAAVKQALTDAENFWVVAKKDDAIKMSKDAMAKVTAVEAAVSA
                     AAPDAQAALGAFKEVGATCTACHMAYRVQNEDKTYSLKPGTI"
     gene            complement(2314..>2846)
                     /locus_tag="AC16CY_23560"
     CDS             complement(2314..>2846)
                     /locus_tag="AC16CY_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3356850.1"
                     /translation="LVGLAPAAHGANRTGRVFTGDGIGGSGDFLMSALHRTGFANIPT
                     SRHPADGLKLKDAYITAAVRCAPPGNKPAPDEITRCLDHLDAEIALLPRLQVVVALGK
                     IAFDAWLQLLARRGVTRSPRPQFGHGGVVRIGEPNKGPQIVIGCYHPSRQNTNTGKLT
                     PAMMAAVFRKARTQLR"
BASE COUNT          463 a          908 c         1032 g          443 t
ORIGIN      
        1 ggcggccttc gacctgggcc ttgccgcggg cgccgacgga ctggagatcg acgtgcacct
       61 gtcgagcgac ggcgtggtcg tcgtctgcca tgacgcgacg ctcgatcgga cgacggacgc
      121 ctcggggccg gtcgcggccc ggacggcggc cgagctggca cgcgtggacg cgggattccg
      181 ctacggcagc aacggcgacc acccgtttcg aggccggggt gtcggcattc ctgcgctgcg
      241 cgacgtgctg cgtcgatatc ccgacactcc gatcatcgtc gagatgaaag tcgatggccg
      301 ggagatgggg ctggctctgg ccgctgacgt gcgcggggcg tcggccatgg accgcgtctg
      361 cgcggcgtcg agcgggtgcc gggcgcttcg ggcggtgcgc gaggcgctgc cggggctggc
      421 gacgagcgcg tgccgctggg acgtccggct tgcgctctat cggtcgtggg tgggatggcc
      481 ggtgcgccag gctgcatacg gcggattcca ggtcccggag gtcgcgggcg ccacgcgaat
      541 cgtgtcgcga gacttcatcc ggcacgccca cgaagggggc ttcgaggtcc aggtgtggac
      601 cgtggacgag gaagccgaca tggaacggct gctggcgtgg ggtgcagacg ggctgattac
      661 caatcgcccg gccgctgcag tggccgtaag agaccgacgt agtgtccggc tttagccgga
      721 ccgtgagcag tggccagtgc gatgaatccg ccgaccttcg aagatgtagt tgccgctcgc
      781 gagcgcgtgt acgcccacat gcgcccgtcg ccgctgctca atcacccgct gctcgacgag
      841 tgggtcggct gcagggtgtg ggtcaagcac gagaatcaca atccgacggg ctcgttcaag
      901 attcgaggcg gactgaatct cgtggcgcag ctctcggccg acgagcggca gcgcggcgtg
      961 atcgccgcga gcaccggcaa tcacggccag tcgctggccc tggcctgcag gctccacggc
     1021 gtgcggtgcc gcattgtcgt gcccgtgggc aacaacccgg acaagaacgc cgccctgcgc
     1081 gcgttcggcg cggagctcgt cgagcacggg cgcgatttcg acgaggcgcg cgagtacgtc
     1141 gaagcggtcg cgggccgcga ggggtggcga tacgtgcact cggccaacga gccgcacctc
     1201 attgccggcg tcggcacgta tgccgtcgag gtcttcgagg cgcagcccgg gatcgatcgc
     1261 atcttcgtgc ccatcggcgg cggcagcggc gccgccgggt gcgcgatcgt gcgaagcggc
     1321 ctgggcctgc cgacgcaaat catcggcgtg caggcacagc aggccgacgc gttcacccgc
     1381 tcgtggcggg ggccggagcg tgtcacccgc gaccgcgtcg ataccttcgc cgaagggctc
     1441 gccaccagga cgaccttcga cctcacgttt ggactgctga aggccacgac cgggctggac
     1501 gaggtggtca cgctcgaaga agaggagctg cgggaaggcg tgactgccgc gatacagcgc
     1561 acgcacaacc tggccgaagg cgcgggggcg gccacgctcg cggccgcccg ccgggccggc
     1621 ctgcccaggg atgcgacggt ggtgtgcgtc atgtcgggcg gcaacgcgga cgcgcggacg
     1681 ctccgtcacg cacttgcctg atggcagggg aatcgggcac gattacgggt cttgcgttcc
     1741 gttcacccgt cataaggagg tcttgatgcg ccgtcttctc acctattcga ccgtcgtgct
     1801 cgtggccctg gcgggcactg tcgcggctca gaaagccacg actcctgcgg agctcgaggg
     1861 agccatgaaa cggatcagtg cagccaatgg cgcggtggcc aaggccatca agtcgggcgc
     1921 cttcgaggac gccaagacgc aggttgccgc cgtgaagcag gcattgacgg atgccgagaa
     1981 cttctgggtc gtcgccaaga aggacgatgc gatcaagatg agcaaggacg cgatggcgaa
     2041 ggtcacggcg gtcgaggccg cggtgtcggc cgcggcgccc gacgcgcagg ccgcgcttgg
     2101 ggcgttcaag gaagtgggtg cgacgtgcac ggcgtgccac atggcgtatc gcgtgcagaa
     2161 cgaggacaag acgtactcgc tcaagccggg gacgatctag actcgacgcg agtcaactcc
     2221 caatctccaa ctcccaactc ccaaggaact cactcccaac gattccacgt cgtttctggg
     2281 agttgggaat tgggcgttgg gagttgacca gaatcagcgg agctgcgttc tcgccttcct
     2341 gaatacggcc gccatcatcg ccggcgtgag cttgccggtg ttcgtgttct gccggctcgg
     2401 gtgataacac ccgatcacga tctgcggtcc tttgtttggc tcgccgatcc gtaccacccc
     2461 accgtggccg aactgcggcc gcggcgatcg cgtgacgccc cgccgagcga gcagctgcag
     2521 ccacgcatcg aaggcaatct tcccgagcgc gacgacgacc tgcaggcggg gaagcagggc
     2581 gatctcggca tcgagatgat cgaggcagcg tgtgatttca tcgggcgccg gcttgttgcc
     2641 gggaggcgcg catcgtacgg ccgccgtgat gtaggcatcc ttcaacttca gcccatccgc
     2701 cgggtgacgc gacgtcggga tgttcgcgaa gccggtccgg tgcagcgcgg acatcagaaa
     2761 atcgcccgac ccgccgatac catcgccggt gaagacgcgg cccgtacggt tggcgccgtg
     2821 cgctgccggc gcgagaccga cgagca
//
LOCUS       DBNGSH010001039         1587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4743736, whole genome shotgun sequence.
ACCESSION   DBNGSH010001039 DBNGSH010000000
VERSION     DBNGSH010001039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4743736"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..722)
                     /locus_tag="AC16CY_23565"
     CDS             complement(12..722)
                     /locus_tag="AC16CY_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356851.1"
                     /translation="MLVLAANGGDPLGAQLRPDFTGVWTDYVDPQQQAGRAGGVPGTA
                     VDLPLTADARQKVESYRKLVAATGDTPGAYCLGPGMPGVLFGGATYPMEIIHRPEQIT
                     IIHELHNDVRRIYLGSRNMAEADRLPGRNGYSSAHWEGDTLVVETTKLVEQVDQRYPH
                     SDRARVIERYELTTSPRGERVLVVDMTMTDPVFYTRPVTGQKKWVEVPNGHLLPYDCA
                     EEGWLKRIEQLEKSATPG"
     gene            complement(995..>1587)
                     /locus_tag="AC16CY_23570"
     CDS             complement(995..>1587)
                     /locus_tag="AC16CY_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HIR3356852.1"
                     /translation="IRTKDYAARGIDISNAMPPGGEGLDRNNPTVARLMNQQRQMVLS
                     MGQFLGEEVLHVSRRIERMDTAVPLSGRFSTISCPGRERTNQGRAGFEGTYKESSPVE
                     IRLGLLRIGDVMIGAVNAEVFNAIAQRLKRESPYKATMLATLTNGSARSGYIPDDASF
                     GKQTFEVLSSRLQPGCAESAIVNGLLSLIDGARGLR"
BASE COUNT          248 a          530 c          516 g          293 t
ORIGIN      
        1 cgccgccgcg gtcacccggg cgttgcgctc ttctcgagct gctcgatgcg cttgagccac
       61 ccttcctcgg cgcagtcgta cggcagcagg tggccgttgg gaacctccac ccatttcttc
      121 tggccggtca ccggccgcgt gtagaaaacc gggtcggtca tggtcatgtc gacgacgagc
      181 acgcgctcgc cccgcgggct cgtcgtgagc tcgtaccgct cgatcacccg cgcacgatcg
      241 ctgtggggat agcgctgatc cacctgttcg accagcttcg tcgtctcgac gaccagcgtg
      301 tcgccttccc aatgcgcgct cgaatagccg ttgcgcccgg ggagccgatc ggcctccgcc
      361 atgttgcggc tgccaagata gatccgtcgg acgtcattgt gcagttcgtg aatgatcgtg
      421 atctgctcgg gcctgtgaat gatctccatc gggtaggtcg caccgccgaa gagcacgccg
      481 ggcatccccg gtccgaggca ataggctccc ggcgtgtcgc cggtcgctgc gacgagcttc
      541 cggtatgact cgaccttctg gcgtgcgtcg gcggtcaacg gcaggtccac ggctgtcccc
      601 ggcactccgc cggcgcgccc ggcctgctgc tgcggatcga cgtagtcggt ccacacgcca
      661 gtgaagtccg gacggagttg tgcgccgagg gggtcgccgc cgttcgcggc gagtaccagc
      721 acaatgaaag aagcctcgag gccgcgaaac acgggactct gaaatcggcg agtgccggcg
      781 gagtgatgac gagcgcggcc gatgtggcct gaggcgagag ctctgtgaac ccgttccacg
      841 atcgatctat atcctagatc gtagtgcctg cagcgcggcg agccgtgcga cacctacgag
      901 gactacggcc tgggcgtcca tgttggcgtt gtagaccgcg ggaatcatcg cgtcaggaag
      961 cccgccgccg tggacgatac cgcggtcccg ggtcttaacg aagccctcgc gcgccgtcga
     1021 tcagcgacag gaggccattg acaatggccg actcggcaca gcctggctgt agccgcgacg
     1081 acagcacctc gaaggtctgc ttcccgaacg acgcatcgtc ggggatgtag ccggagcgcg
     1141 ccgaaccgtt cgtcagcgtc gccagcatgg ttgccttgta cggcgactcg cgcttcagcc
     1201 gctgcgcgat ggcgttgaac acctccgcgt tcaccgcgcc gatcatcacg tcgccgatgc
     1261 gcaggagacc gagcctgatc tccaccggac tgctttcttt gtaggtgcct tcgaagccgg
     1321 cccggccctg attcgtccgc tcgcgtccgg gacagctgat cgtcgagaag cggccggaga
     1381 gcggcaccgc ggtgtccatg cgctcgatgc ggcgactgac atgcagcacc tcctcgccga
     1441 ggaactgccc catcgagagc accatctgcc gctgctggtt catgagtcgt gcgacggtcg
     1501 gattgttgcg gtccagccct tccccaccgg gtggcatggc gttgctgatg tcgatgcctc
     1561 gcgcggcgta gtccttcgtc cgtatgt
//
LOCUS       DBNGSH010001040         5973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3892727, whole genome shotgun sequence.
ACCESSION   DBNGSH010001040 DBNGSH010000000
VERSION     DBNGSH010001040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3892727"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..2439)
                     /locus_tag="AC16CY_23575"
     CDS             complement(160..2439)
                     /locus_tag="AC16CY_23575"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3356853.1"
                     /translation="MHPDVIAHYKIGEKLGHGGMGEVYRATDTKLGRQVAVKFLPDSL
                     ATDPAQMARFTREAQVLASLNHPNIAAIYGVEDRALVMELVEGPTLSDRIKTGAVPLE
                     EALRIAGQIVDALEAAHAKGIVHRDLKPANIKVTRDGTVKVLDFGLAKVAIAESTEAT
                     DTSPTMTTTQAGLILGTAGYMSPEQARGKEVDKRADIWAFGVVLYEMLTGTQLFQGDT
                     VTDVMAAVVRQDPDFTKVPAKVQPLLRRCLEKDPKRRLRDIGDAMLLLDAGAALSASP
                     APARTSSWSRISAAAAVLFLAAFAAVAFVHFREAPPAADAVRFQVPYPSDLSPVPSGV
                     FALSPDGRTIAYGAFGTDGIARIWLRPLDRLEATSLPGVEINRNTLALFWSPDGRHLA
                     YWAGQKLQRIDVAGGSPQVIADAAMGTLGGSWSRDGIIIYGSGAGIMQVPASGGTPTA
                     VTKTGGGQGDLHVMPYFLPDGRHFLYLRTGAPESRGVHVGSLDAKPEEQNATPLVKTD
                     NSAIYAPGQDGRIGQLLFVRGSTLLAQPFDAARLELLGEPVPVADPIAASNFGFGGVA
                     FVSTSNTGTLLYFRPASQNVQLTWFDREGQPGRPAGEPGRWGVMKLSPDGTRAAIVRN
                     DGNNQDIWQIDLSKGTSTRFTFDPGGDTQPVWSADGSRIAWISNRDGFTGIYAKPADG
                     SGTEELLHKFKTRPTSLTDWTRDGRFLVYTDGGDIWALPVSGVPHEKREPIPVIQGER
                     GQLGAYVSPDQRWIAYISN"
     gene            2481..3122
                     /locus_tag="AC16CY_23580"
     CDS             2481..3122
                     /locus_tag="AC16CY_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014873039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3356854.1"
                     /translation="MHQILVYGDSLTWGIIPDTRQRWPFETRWPGVMERALGKAGHDV
                     RVIEDCLNGRRTAWDDPFKPGRNGRIGLEQRIEVNSPLALVVLLLGTNDFQSVHDVNA
                     GHSAQGIASLVRAIKLAPIEPGMPAPKILIVAPPEIHEAKGVMAAKFEGARAKCAGLA
                     AAYAAVARELGCSFFDAGTVTTTSRIDGVHLDADQHERMGAALAAVAGPLLPG"
     gene            3169..4932
                     /locus_tag="AC16CY_23585"
     CDS             3169..4932
                     /locus_tag="AC16CY_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3356855.1"
                     /translation="MSTSPQHTGTSRREFLTSTAAAGLGAASCAPQIAGGGASPAAAQ
                     ALVGDGSKRRILLRGGVVLSLDTKVGDFERADVLIDGKRIAQIAPSVSAADAEVIDCA
                     GTIVMPGFITTHHHQYETLQRSIIADGLLGGAWPMESYGSVVQNIWTAGRIADPKDPS
                     RFIWDLGRVPYDPEDCYISQLVACLSEISEGITTGTDTSQANHTPDHTDAMIKGLMDS
                     GRRTVFAYSGGIDRSAAGIPFEYPGAMNDTTKGIGRIAKTYFSSKDQLVTLGFAGGTG
                     PAFAGASYTGWQLGRSFGASLHNHVVGNPMGIVNAAADARNGSDWSDVTFIHATRWQE
                     TPRAQVGPGASTYPGGSRSRPWEICRERGAHISIAVLIEMQMRHGMPPFQEALNHGIL
                     PSLSPDVDSNMTTDPFSLMRGAFCLQRALANDLAFTESNPGGLPIPQLVTSRQVIEMA
                     TIAGAASNRVLDKVGTLTPGKEADIVVLDARNINTWPMNNVPGTVVTMMNPRHVRDVL
                     IAGRVVYWNRKHVGWDIERLLVQIEQARERVLARIGGPARVGSLPPGNNSLANPYRPN
                     LLGNCCFKGQNTDAPEYVLRP"
     gene            4934..>5973
                     /locus_tag="AC16CY_23590"
     CDS             4934..>5973
                     /locus_tag="AC16CY_23590"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3356856.1"
                     /translation="MTSRRAFLSHVAGATAGCLLAGGDLVNAGLRMQVGAPPGKRREL
                     VIGGRRVKTVDVHAHCFVPEVWDVVKDTPLAAAAKGSLTGNIALGNPQRLIDMDAQGI
                     DVQAINVNAWGYSADRALATDLVKVQNEKISQWCAAHPDRFVGMATVALQHPELAADQ
                     LDEAVRKLGLRGAAIGGSVEGQELSDRKFDPFWAKAEQLGVLLFMHPQAAQGTTQNPR
                     LQGRGGLGNTIGNPLETTVFLSHLIFEGTLDRFPGLRICAAHAGGYLASYTGRSDALC
                     GRGGGLGADCRALKKKPSEYFRRELLIDTMIFREEGLRHLVAEAGVGQIVYGTDYPFD
                     WPVGIDFVLNAP"
BASE COUNT          979 a         2015 c         2015 g          964 t
ORIGIN      
        1 gtccgtgccg aggaagatca gctccctgcc gtcggcgcgc catcgcgcca tgccgagcgt
       61 gccgttcgaa gacaccatcc acttgcccga caccggctgt cccttcgcgc ccggcgctcc
      121 cggggcaaac ggctgcacgt acatttcctg ctggcccgac tagttcgaga tgtacgcgat
      181 ccaccgctgg tcgggggaga cgtacgcgcc gagttgtccc cgctcgccct ggatcactgg
      241 tatcggctcg cgcttctcat gcggaacgcc ggacaccggg agcgcccaga tgtcgccccc
      301 gtcggtgtag acgaggaagc gcccgtcgcg cgtccagtct gtcagtgatg tcggtctcgt
      361 cttgaacttg tggagcagtt cttcggtgcc cgacccgtcg gccggcttcg cataaatgcc
      421 agtgaagccg tcgcggttcg aaatccacgc gatccggctt ccgtcggccg accagaccgg
      481 ctgcgtgtct cccccgggat cgaacgtgaa ccgcgtgctc gtccccttgc tcaagtcgat
      541 ctgccagatg tcctggttgt tcccatcgtt gcggacgatc gcagccctgg ttccgtctgg
      601 tgagagcttc atgactcccc agcgtccggg ctcgcctgcc ggacggcctg gctgcccttc
      661 gcggtcgaac catgtgagct gcacgttctg cgatgcgggc ctgaagtaca acagcgttcc
      721 ggtgttcgac gtcgagacga aggcgactcc gccgaatccg aagttcgatg ctgcgatcgg
      781 gtctgcaact ggaacgggct cacccagaag ctcgagccgc gcggcgtcga agggctgcgc
      841 cagcagcgtg ctgccgcgga cgaacaggag ctgcccgatc ctgccgtcct gtccgggtgc
      901 gtagatggcg ctgttgtcgg tcttgacgag cggcgtcgcg ttctgctcct ccggcttcgc
      961 gtcgagcgat ccgacatgaa ccccccggct ctcgggagcg ccggtccgaa ggtagagaaa
     1021 gtgccggccg tcggggagaa agtacggcat gacgtgcaag tcgccctggc cgccgcccgt
     1081 cttggtgacc gcagtcggag tcccgccgga ggccggcacc tgcatgatcc ctgcgccgct
     1141 tccgtagatg atgatgccgt ctcgactcca ggagcccccg agcgtaccca tcgcggcatc
     1201 ggcgatgacc tgcggggacc cgccggcgac gtcgatgcgc tgcagcttct ggccggccca
     1261 gtacgccagg tgccggccgt cgggcgacca gaacagcgcg agcgtgttgc ggttgatctc
     1321 gactcccggc agtgaggtgg cttccagccg atccagcggg cgcagccaga tgcgcgcgat
     1381 gccgtcggtc ccgaacgcac catacgcgat cgtgcggccg tcgggcgaca gagcgaacac
     1441 gcccgaggga accgggctca gatcggatgg gtagggaacc tggaaccgca cggcgtccgc
     1501 cgcggggggc gcttcgcgaa agtggacgaa cgcgacggcg gcgaatgccg ccaggaacag
     1561 caccgcggcc gcggcgctga tgcgcgacca cgacgatgtt cgagcgggcg ccggcgacgc
     1621 ggaaagcgcg gccccggcat ccagaagcag catcgcgtcg ccgatgtctc tcagccgccg
     1681 cttcggatcc ttttcgagac atcgcctgag cagcggctgg accttcgccg ggactttggt
     1741 gaagtcggga tcctggcgca cgaccgcggc catcacgtcg gtcacggtgt cgccctggaa
     1801 cagctgcgtg ccggtgagca tctcgtagag caccacaccg aacgcccaga tgtcggcgcg
     1861 cttgtccacc tctttgccgc gagcctgttc aggcgacatg tagccggcgg tccccaggat
     1921 gagtcccgcc tgcgtcgtcg tcatcgttgg cgacgtgtcc gttgcctcag tgctttccgc
     1981 gatggcgacc ttggccagcc cgaagtccag caccttgacc gtgccgtcgc gcgtgacctt
     2041 gatgttggcg ggcttcaaat cgcgatggac gatgcccttg gcgtgcgcgg cttcgagcgc
     2101 gtccacgatc tggcccgcga tgcgcagcgc ctcctcgagt ggaaccgcgc cggtcttgat
     2161 gcggtccgag agcgtcgggc cctcgaccaa ctccatcacc agtgcgcgat cctcgacgcc
     2221 gtagatcgcg gcgatgttgg ggtggttcaa cgaggccagc acctgcgcct cgcgcgtgaa
     2281 gcgcgccatc tgtgcgggat cggttgccag gctgtccggg aggaacttca cggcgacctg
     2341 gcgtccgagc ttcgtatcgg tcgcccggta cacctcgccc atgccgccgt ggccgagctt
     2401 ttctccgatc ttgtagtgcg cgatgacgtc tggatgcacg atgcccgcct cgggatgcga
     2461 atggtagcat ctctggcgtc gtgcaccaga ttctcgtcta cggcgactcg ctgacgtggg
     2521 gcatcatccc cgacacccgc cagcgatggc ccttcgagac gcgctggccc ggcgtcatgg
     2581 aacgcgcgct cggcaaggcc ggccacgacg tccgcgtgat cgaggactgc ctgaacggac
     2641 ggcggacggc gtgggacgac ccgttcaagc ccgggcgcaa cggacgcatc ggcctcgagc
     2701 agcgcatcga ggtcaactcg ccgctcgcgc tggtggtgct gctgctgggg acgaacgact
     2761 tccagtcagt gcacgacgtc aacgccggtc actcggcgca ggggatcgca tcgctggtgc
     2821 gcgcgataaa gctcgctccc atcgagccgg gcatgcctgc accgaagatc ctgattgtcg
     2881 cgcctccgga aattcacgag gccaagggcg tcatggctgc gaagttcgaa ggcgcgcgcg
     2941 cgaaatgcgc cgggcttgcg gcggcgtacg cggccgtggc ccgcgagctg gggtgttcgt
     3001 tcttcgacgc ggggacggtc acgacgacca gccgaatcga cggcgtccat ctcgacgccg
     3061 atcaacacga acgcatgggc gcagcgcttg ccgccgtcgc aggaccgctg ctgcccggtt
     3121 agcgcgggga ttccaatcga ccgtttcgac tcgtcaggga ggttcgcgat gtccacgagt
     3181 ccgcagcaca caggcacctc acgccgggag ttcctgacct cgacggccgc ggccggcctc
     3241 ggcgccgcgt cctgcgcacc gcagatcgcg ggcggcggcg cgagcccagc cgccgctcag
     3301 gcgctcgtcg gcgatggcag caagcggcgc attctcctgc gaggcggcgt cgtgctcagc
     3361 ctcgacacga aggtgggcga cttcgagagg gccgacgtcc tcatcgacgg caagaggatc
     3421 gcgcaaatcg cgccgagcgt gtccgccgct gatgccgagg tcatcgactg cgccggcacc
     3481 atcgtcatgc ccggcttcat cacgacgcac caccatcaat acgagacgct gcagcgcagc
     3541 atcatcgccg acggcctgct cggcggggcg tggccgatgg aaagctacgg gtcggtcgtg
     3601 cagaacatct ggaccgctgg ccggatcgcg gatccgaagg acccgtcccg attcatctgg
     3661 gacctgggcc gcgtgccgta cgaccccgag gactgctaca tctcccaact cgtggcctgc
     3721 ctgagcgaga tcagcgaggg catcacgacc gggacggaca cgtcacaggc caaccataca
     3781 cccgaccaca ccgacgccat gatcaagggg ctgatggact cgggccggcg cacggtgttt
     3841 gcctacagcg ggggaatcga ccgcagcgcc gcgggcattc ccttcgagta tcccggcgcg
     3901 atgaacgaca cgacgaaagg gatcggacgg atcgcgaaga cctacttcag ctcgaaagac
     3961 cagctggtga cgctcggctt cgccggcggc accggaccgg cgttcgcggg cgcgtcgtac
     4021 accggctggc agctcggtcg ctccttcggc gcatcgctcc acaaccacgt cgtcggcaac
     4081 ccgatgggga tcgtcaatgc ggccgcggac gcgaggaatg gctcggactg gtccgacgtc
     4141 accttcatcc acgcgacccg gtggcaggag acaccgcgcg cccaggtcgg ccccggcgcg
     4201 tccacctacc cgggcggcag ccgttcgagg ccgtgggaga tctgccggga gcgaggtgcg
     4261 cacatctcga tcgccgttct catcgagatg cagatgcggc acgggatgcc tccgtttcag
     4321 gaggcgctca accacggcat cctgcccagc ctgagcccgg acgtcgactc gaacatgacg
     4381 accgacccgt tctcgctgat gcgcggggcg ttctgcctgc agcgggcgct ggccaacgat
     4441 ctggcgttca cggaaagcaa cccgggcggc ctgccgatcc cgcagctcgt cacgtcccgt
     4501 caggtgatcg agatggcgac catcgcgggg gcggcgtcca atcgcgtcct cgacaaggtc
     4561 ggcacgctga cgccggggaa ggaagcggac atcgtcgtcc tcgatgcgcg caacatcaac
     4621 acgtggccga tgaacaacgt gccgggcacc gtcgtgacga tgatgaaccc gaggcacgtc
     4681 cgcgacgtgc tgatcgcggg acgtgtcgtg tactggaacc ggaagcacgt gggatgggac
     4741 atcgagcgcc tcctcgtcca gatcgaacag gcgcgggaac gcgtgctcgc acggatcggc
     4801 ggcccggcca gggtgggatc gcttccgccg ggcaacaaca gcctggccaa cccctaccgg
     4861 ccgaatctgc tcggaaattg ctgcttcaag gggcagaata cggacgctcc cgagtatgtg
     4921 ctgaggccct gacatgacca gccgacgagc ctttctctcg cacgtcgccg gcgcgacagc
     4981 cggatgcctt cttgccggag gcgatctcgt caacgccggc cttcgaatgc aggtcggcgc
     5041 cccgcccggc aagcgccgcg aactcgtcat cggcgggcgt cgcgtgaaga cggttgacgt
     5101 gcacgcgcac tgcttcgtgc cggaggtgtg ggatgtcgtg aaggacacgc ctcttgccgc
     5161 ggccgcgaag gggagtctca ccggcaacat cgcgctcggg aacccgcagc gcctgatcga
     5221 catggacgcg caaggcatcg acgtgcaggc gatcaacgtc aacgcctggg gatattcggc
     5281 agaccgcgcg ctggccaccg atctcgtcaa ggttcagaac gagaagatct cccagtggtg
     5341 cgcggcccat ccggatcggt tcgtggggat ggcgaccgtg gccttgcagc atcccgagct
     5401 cgccgccgat cagctggacg aggcggtcag aaagctgggg ctgcgcggcg cggccatcgg
     5461 agggagcgtc gaagggcagg agctgtccga tcggaagttc gatcctttct gggcgaaggc
     5521 ggagcagctg ggcgtgctgc tgttcatgca tccgcaggcc gcccagggca cgacgcagaa
     5581 tccgcgcctg caggggcggg gcgggctggg gaacacgatc ggcaatcctc tggaaacgac
     5641 ggtgttcctc tctcacctga tcttcgaggg cacgctcgat cggttccccg gcctcaggat
     5701 ctgcgcggcg cacgccggtg gatacctggc gtcgtacacc ggccgatcgg atgcgctctg
     5761 cggccgcggg ggcggcctcg gcgccgactg ccgcgcgctc aagaagaagc cgagcgagta
     5821 tttcaggcgc gagctgctga tcgacacgat gatcttccgc gaggagggcc tgcggcacct
     5881 cgtcgccgaa gcgggcgtcg gccagatcgt gtacggaacc gactatccct tcgactggcc
     5941 cgtcgggatc gacttcgtcc tcaacgcgcc ctt
//
LOCUS       DBNGSH010001041         1803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_838312, whole genome shotgun sequence.
ACCESSION   DBNGSH010001041 DBNGSH010000000
VERSION     DBNGSH010001041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_838312"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="AC16CY_23595"
     CDS             <1..402
                     /locus_tag="AC16CY_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HIR3356857.1"
                     /translation="VIFALSNPTSKAECTAEQAYTWSDGRAVFTSGSPFGTVVYRGVS
                     RRPGQGNNAYVFPGIGLGAVACRARTLAGLVSEKDLAQGCLYPPLTDIRKISLAIAVR
                     VAGKAYAMKLARGRRPADLRKNIARLMYGPA"
     gene            complement(467..>1803)
                     /locus_tag="AC16CY_23600"
     CDS             complement(467..>1803)
                     /locus_tag="AC16CY_23600"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3356858.1"
                     /translation="HHTPLEGAASIAEIQGILKARAKSVPAGAFITSMGGWNQAHFAE
                     KRLPTLAELDAAAPNHPVLVYQSFTGPASTNTRGLAFFKSKGVAVSDTGAIGANAPSL
                     AALNALRAEQTFEDRKRGTTEAMAYSASVGVTTSADMGAFNLPGTQNLQGSFEADTLA
                     SADQFRMYDAFAALHREGRLTTRLRIFFLTMDMRPEVPILSERLRNDFLGFGDDMMRI
                     SGIGEFASSWPLFGQKPPTNYEAALTAIAKQGWAFQQHSLSPAENELTVSTFEAVNKT
                     VPIANLRWSLAHVGSVDKATIDRLKAMGAAIAVHPFQFLAGGRGGPPLRTIVDSGIKV
                     GAGSDSAQISTLNPWLVISYMVTGKALDGSLINKGEQLTRMEALRLYTAENGWFLKEE
                     ATIGTIEAGKLADVVVLTDDYFDAARVPDDAIKRIKSVLTVVDGKVVHNTMQ"
BASE COUNT          272 a          607 c          616 g          308 t
ORIGIN      
        1 gtgatctttg cgctgtccaa tccgacgtcc aaggccgagt gcacggccga acaggcgtac
       61 acctggagcg acgggcgggc ggtgtttacg agcggcagcc cgttcggcac cgtcgtgtac
      121 cggggcgtgt cgcgccggcc cggccagggg aacaatgcgt acgtgttccc ggggatcggc
      181 ctgggcgccg tggcgtgccg cgcccggacg ctggcgggcc tcgtcagcga aaaggacctc
      241 gcgcagggat gcctctaccc gccgctcacg gacatccgga agatctcgct cgccatcgcg
      301 gtccgcgtgg ccggaaaggc gtatgcgatg aaactggcgc gcgggcgccg gccggcggac
      361 ctccggaaga acatcgcgcg cctgatgtac ggaccggcgt gagcgacgag accgaggcca
      421 tgtggaatct ccgattcgcg cgacagcccg agtcctgcag gcccgcctac tgcatcgtat
      481 tgtgcacgac cttcccgtcc acgacggtca gcactgactt gatccgcttg atcgcgtcat
      541 ccggcacgcg cgcggcgtcg aagtagtcgt cggtcaggac gacgacgtcg gccagcttgc
      601 cggcctcgat cgtgccgatc gtcgcctctt ccttcaggaa ccacccgttc tcggcggtgt
      661 acaggcgcag cgcctccatg cgcgtgagct gctcgccctt gttgatgagc gatccgtcca
      721 gcgccttccc ggtcaccatg taggagatca cgagccacgg gttgagcgtc gagatctgcg
      781 ccgaatccga ccccgcgccc accttgatcc cgctgtccac gatggtccgc agcggcgggc
      841 cgccgcggcc gccggcgagg aactgaaacg ggtggacggc tatggccgcc cccatcgctt
      901 tcagccggtc gatcgtggcc ttgtcgaccg accccacgtg cgcgagcgac catcgcaggt
      961 tggcgatcgg gacggtcttg ttcaccgcct cgaaggtgct gacggtcagc tcgttctccg
     1021 cgggagagag gctgtgctgc tgaaacgccc agccctgctt cgcgatcgct gtgagcgccg
     1081 cctcgtaatt ggtcggaggc ttctgtccga acaagggcca gctcgacgcg aactcgccga
     1141 tgcccgagat ccgcatcatg tcgtcgccga agccgaggaa gtcgtttcgc aggcgctccg
     1201 acaggatcgg cacctcgggc cgcatgtcca tcgtcaggaa gaagatgcgc aggcgcgtcg
     1261 tcaatctgcc ctcgcggtgg agcgcggcga aggcgtcgta catccggaac tgatcggcgc
     1321 tggccagcgt gtcggcctcg aaggagccct gaaggttctg tgtgcccggc aggttgaacg
     1381 cgcccatgtc ggcgctcgtc gtcacgccga cactcgcgga gtacgccatc gcctcggtgg
     1441 tgccgcgctt gcggtcctcg aaggtctgct cggcgcgaag cgcgttgaga gccgccaggg
     1501 acggggcgtt ggcgccaatc gcgcccgtgt cgctcaccgc cacgcccttc gacttgaaga
     1561 aggccaggcc tcgcgtgttg gttgaggcgg gtcccgtgaa ggattggtag acgagaaccg
     1621 ggtgattggg cgccgccgcg tcgagctcgg ccagcgtcgg cagccgtttc tccgcgaagt
     1681 gcgcctggtt ccacccgccc atcgacgtga tgaacgcgcc cgcggggacg ctcttggccc
     1741 tggccttcag aattccctgg atctccgcga tcgacgcggc gccttcgagc ggcgtgtggt
     1801 ggc
//
LOCUS       DBNGSH010001042         3879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_329558, whole genome shotgun sequence.
ACCESSION   DBNGSH010001042 DBNGSH010000000
VERSION     DBNGSH010001042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_329558"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2571
                     /locus_tag="AC16CY_23605"
     CDS             <1..2571
                     /locus_tag="AC16CY_23605"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3356859.1"
                     /translation="SDADRVSRFQREAQVLASLNHPNIAAIYGLEESSSGRSSDPPTR
                     ALVLELVEGPTLADRLALEPLALEEALSIARQMAEALEAAHAHGVIHRDLKPANVKVR
                     PDGLVKVLDFGLAKILEASGANEASSVMGLSLSPTMTSPAMTRMGLIMGTAAYMSPEQ
                     ARGSLVDSRTDVWSFGVVLYEMLAGKTLFEGPTVSDTLAAVLRADLDWDRLPSNLPPD
                     VQRLLRRCLQRDPKKRLQHIGDARVEIEDLQTGPVRESPETPTGKRWGLAAAAALLTA
                     LVVAPAVWTLKPLPAASVLATSFVIRTPSEAPVGRNSPLALSPDGQQLVYTVGLGSDF
                     VSRLFHQSLNAFDPRSLEGTDASSPFFSPDGSAVGFFSRSSNQLQQLRLAGGGATRLV
                     SAANSVGASWGDDETIVFAEDWGLPLRVTQLGTSATRDLTRLNVSAGERAHLWPQLLP
                     SNRAVLFTVWTAAPTWDESLLAVADLETGTHRIVLRGGAFGRYSSSGHLVFWRDNALM
                     AVPFDLSRLAVSGDPVKVVSGVRLDNSNGGAHFALSSTGTLAYVAGGVDTFAEGFLAD
                     RSGRQVVRLDEMVPVGDPAFSPDGKRVALTLYKGGTFGIGVYDLERHLLTPLPLAGDN
                     LRPSWTMKGDRVTFVSNASGAYNYHSMPFDGSGAPEALFTPEQAAQGGLLASAVWSPD
                     GRQLLYGKSGGKTGADIWIHTPGQKAAPKALIATGATESNPAFSPDGRFIVYQSDESG
                     TVEVYVRPFPNVDASRELISRSGGTRPTWSREGTEIFYLSGKGMMRVPVTYGRGDASR
                     PSFGQPSEALAMSGLSSFDVSPDGRMFAIERVPIEKAATEIRVVVNWFEELRRLVPAR
                     "
     gene            2645..3625
                     /locus_tag="AC16CY_23610"
     CDS             2645..3625
                     /locus_tag="AC16CY_23610"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR3356860.1"
                     /translation="MRSTVLMFVAVSDSVFPNLDPARGVLRAIGAELQLAADSSPASI
                     MAIASGADALLVTYAKITADMIGQMPRCRIISRFGIGVDNVDLQAATGAGIVVTKVPD
                     YCIDEVSDHTLALLLALVRKIPSSSARTHSGRWEMKAVVPIHRLRGSVLGLVGFGRIP
                     QLVAPKAQSFGMRVLAYDPFVPRDVFARAGVEGVELAELLTRSDYVSIHTPLMPETRG
                     LFNRDTFGRMKRGAYLVNTARGPIVDEAALAEALDAGQLAGAALDVMTEEPPPRSPLF
                     GRDNVIITPHTSFYSEESLVELQVKAAQEVVAVLSGKAARNPVNPEVVTR"
     gene            3622..>3879
                     /locus_tag="AC16CY_23615"
     CDS             3622..>3879
                     /locus_tag="AC16CY_23615"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3356861.1"
                     /translation="MKAMVLTAPGELARQDVARPGPASGDVLVHVTHSGICGTDYKIF
                     GGAIPVRYPRIMGHEMAGDVVDAGASTLVGGDRVIIDPQLYC"
BASE COUNT          640 a         1273 c         1294 g          672 t
ORIGIN      
        1 agcgacgctg accgcgtgtc tcgattccag cgcgaggcgc aggtcctcgc ctcgctcaac
       61 caccccaaca tcgctgcgat ctacgggttg gaggagtcca gtagcggccg gtcttcagac
      121 ccgccgacca gggcgctggt cctcgagctc gtcgagggcc caacactcgc agatcggctg
      181 gcgcttgaac cgctggcgct cgaggaggcg ctgtcgatcg ccaggcagat ggccgaggcg
      241 ctggaagccg cacacgcgca cggcgtcatc caccgcgacc tgaagccggc caacgtgaag
      301 gtgagaccgg acggcctggt aaaagtgctc gacttcggcc tcgcaaagat cctcgaggcg
      361 agtggggcga atgaggccag ctcggtgatg gggctgtcgc tgtcgccgac aatgacgagt
      421 ccggcgatga ctcggatggg cctgatcatg ggcacggccg catacatgag ccccgagcag
      481 gcgcgcgggt ccctcgtcga ctcacggacc gacgtgtggt cgttcggagt cgtgctgtac
      541 gagatgctcg cggggaagac gctgttcgaa gggccaacgg tgtccgacac gctggcggcc
      601 gtgcttcgcg ccgatctcga ctgggatcga ttgccgtcca accttcctcc cgacgtccag
      661 cgtctgcttc gacgctgcct tcagcgggat ccgaagaaac ggttgcagca catcggcgac
      721 gcgcgggtcg agatcgaaga ccttcagacg ggaccggtcc gcgagtctcc cgagacaccg
      781 acgggaaaac ggtgggggct cgccgctgca gccgctctgc tgacggccct cgtcgtggcc
      841 ccggccgtgt ggaccctcaa gccgctgcct gcggcaagcg tcctggcgac gagcttcgtc
      901 ataagaacgc cttccgaggc acccgtgggc cggaactctc ccctcgcgct gtcgcccgac
      961 ggccagcagc tcgtgtacac agtcggcctc ggctctgact ttgtcagccg cctctttcac
     1021 cagagtctga acgcgttcga tccgcgctcg ctcgaaggca ctgatgcgag cagtccgttc
     1081 ttctctcctg acggcagcgc cgtagggttc ttctcccgat cctccaacca gctgcagcaa
     1141 ctgaggctgg ctggaggcgg cgcgacaagg ctcgtcagcg cggctaactc agtcggtgca
     1201 agctggggag acgacgagac cattgtcttc gcggaagact ggggactgcc cttgcgggtg
     1261 acacagctcg gcacctccgc aacgcgcgac ttgactcgac tgaacgtctc cgccggcgag
     1321 cgtgcgcatc tctggcctca gctcctcccc agcaaccgtg cggtgctgtt caccgtctgg
     1381 accgcggcgc cgacgtggga cgagtcgctc ctggcggttg cggacctcga aacaggcacg
     1441 cacaggatcg tgcttcgggg aggggcattc gggcggtaca gctcgagcgg gcacctggtg
     1501 ttctggcgtg acaacgcact catggccgtg ccgttcgacc tgagccgact ggcggtttcg
     1561 ggagatccgg tcaaggtcgt ttcaggcgta cgcctcgaca attccaacgg cggcgcccac
     1621 ttcgctctgt cgagcacggg cacgctggcc tatgtcgcgg gcggcgtcga cacgtttgcc
     1681 gaaggcttcc tcgccgaccg ctcgggccgg caggtcgttc gtctcgacga gatggtcccg
     1741 gtcggcgatc cggctttttc gccagacggc aagcgagtgg cgttgacgct ctacaagggc
     1801 ggcacgttcg gaatcggcgt ttacgatttg gagcggcacc tgctgactcc gctgccgctg
     1861 gccggcgaca atctgcgccc atcgtggacg atgaagggcg atcgcgtgac cttcgtgtcg
     1921 aacgccagcg gcgcgtacaa ctaccattcg atgccattcg acggcagcgg tgcacccgag
     1981 gcgctgttca cgccggagca ggccgcgcaa ggcggcttgc tagccagcgc cgtatggtcg
     2041 cccgatggga ggcagctctt gtacggcaag tcgggtggga aaaccggagc ggatatctgg
     2101 atccacacgc ccggccagaa ggcagctccg aaggcactca tcgcaacggg tgcgacggag
     2161 agcaacccgg cgttttcacc ggacggccgt ttcatcgtgt atcaatcaga tgaatccggc
     2221 acggtcgaag tctacgttcg gccctttccg aatgtcgacg caagccggga actgatttcc
     2281 aggtcgggtg gaacgcgccc gacgtggagt cgcgaaggca ctgagatctt ctatctctca
     2341 ggcaagggga tgatgagggt cccggtcact tacggtcgtg gcgacgcttc gcgaccttcg
     2401 ttcggtcagc cgtcggaagc cctcgcgatg agcggcctct cgagcttcga cgtctccccg
     2461 gacggccgca tgtttgccat cgaacgggtg cccatcgaga aagcggccac ggagatccgc
     2521 gtggtggtca actggttcga ggagctcagg cgcctcgtgc ccgcgcggtg acacgcgatg
     2581 gactagacta acaacgcgca cgctgaagca tgcgctctac ggttgtcaat gcgcacgctg
     2641 aagcgtgcgc tctacggtgc ttatgttcgt cgctgtttct gattccgtct ttccgaacct
     2701 ggatccggcc cgcggggtac tgcgggcgat cggagccgag ctgcagctgg ccgccgattc
     2761 gtcgcccgcg tcgatcatgg cgatcgcctc cggtgccgat gccctgttag tcacctacgc
     2821 caagatcacg gcggacatga ttgggcaaat gccacgctgc cggatcattt cccgcttcgg
     2881 catcggcgtg gacaacgtcg atctgcaggc cgccacaggt gccgggatcg tcgtgacgaa
     2941 ggtacccgac tactgcatcg acgaggtctc ggatcacacg ctcgcgctgc tgctggcgct
     3001 cgtcaggaag atcccgtcgt ccagcgcccg tacccattcg ggccgatggg agatgaaagc
     3061 cgtcgtcccg atccaccggc tgcgcggcag cgtgctcggg ctcgtcggct tcggcaggat
     3121 cccgcagctc gtcgccccca aggcgcagtc gttcgggatg cgcgtgctcg cgtacgaccc
     3181 gttcgtgccc cgggacgtgt tcgcccgcgc gggcgtggag ggcgtcgagc tggccgaact
     3241 gctgacgagg tcggactacg tctcgatcca cactccgctg atgcccgaga cgcgcggtct
     3301 cttcaaccgc gacacgttcg gccggatgaa gcggggcgcg tatctggtca acacggctcg
     3361 ggggccgatc gtcgatgaag ccgcgctggc cgaggcgctc gacgcggggc aactggccgg
     3421 ggccgccctg gatgtcatga ccgaggagcc gccgccgcgc tcgccgctct tcgggcgcga
     3481 caacgtgatc atcaccccgc acacgagctt ctattccgag gagtcgctcg tcgagctgca
     3541 ggtcaaggcg gcacaggagg tcgtggcggt gctcagcgga aaggcggcac ggaaccccgt
     3601 caatccggaa gtggtcacgc gatgaaggcg atggtcctca cggcgccggg agaactggcc
     3661 cgccaggacg tggcgcgccc cgggcccgcc tccggcgacg tcctcgtcca cgtgacgcac
     3721 agcggcatct gcgggaccga ctacaagatc ttcggcggtg caattcccgt ccgctatccg
     3781 cgaatcatgg ggcacgagat ggcgggcgac gtggttgacg ccggcgccag cacgctcgtc
     3841 ggcggcgatc gcgtcatcat cgacccgcag ctctactgc
//
LOCUS       DBNGSH010001043         9777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2877028, whole genome shotgun sequence.
ACCESSION   DBNGSH010001043 DBNGSH010000000
VERSION     DBNGSH010001043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2877028"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..2007
                     /locus_tag="AC16CY_23620"
     CDS             316..2007
                     /locus_tag="AC16CY_23620"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR3356862.1"
                     /translation="MQIAAIDVGTNSVHMIVVRVRPDLSFEVVDREKVMVRLGAGGLD
                     GKALTSEAMSSSLQALSKFKRLAESHGVDVILAAATSATREARNGGEFLARIEAETGI
                     RPRVITGAEEARLIHQAAVYGVDVGSARAVVIDIGGGSVEITHGTATQIQAARSFKIG
                     VIRLTERFIHSDPLSGRDEEKLTRHVLNEIDRHCEQITAAGFDRVIGTSGTIMSLGAV
                     AVALTTGTPPSELRNLRVSANQIRRLRKEIVGLSPEQRLALAGLDPRRADLAVAGALL
                     LDLILRRLDAESLTLCDLALREGLVLDYIRRNSKQIARAERIPDVRRRSTIELAERCS
                     YYAEHSQQVARLALAIFDQTRAVHGLTDREREWLEFASLLHDIGSLISFSRHHRHSYY
                     LIRNGGLRGFQPDEIEVMALVARYHRRGTPKRSHDEYARLPAPLRRIVRMLSSILRVA
                     ECLDRSHTQAISGLDLRDRGKDVLIQVHTSADAELEIWATNRHLKPFEKLVGKPVHLE
                     AAAVPSVAVPERASAEKVKKTAGHSRGNGHSRPRKVVVSPRQPAPASSRAPERRT"
     gene            2099..3112
                     /locus_tag="AC16CY_23625"
     CDS             2099..3112
                     /locus_tag="AC16CY_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3356863.1"
                     /translation="MVIRVSGLVLAVVSLAACSGGSNAGESGAPAMIQIDGSSTVFPI
                     TEAVAEEFQKTDTAVRVTVGISGTGGGFQKFCRGETDISNASRPIRPTEIETCSKAGI
                     EYIELPVAYDGLAIVVNPKNTWADQVAVSELKTLWAPEAQGKILRWNQVRGSWPDREI
                     RLFGAGVDSGTYDYFTEATVGKEGTSRGDFTSSEDDNVLVQNISTDELALGFLPLAYV
                     EQNQSKLKLVPVDDGKPDNGKGPITPTAATVRDGTYQPLSRPLFIYVARKAADRPEVQ
                     RFVQAYLDSAPLMREVGYVELTPQIYDLARKHFSDRRVGTAFGAGGSQVGVTLEQLLT
                     REQ"
     gene            3109..3984
                     /gene="pstC"
                     /locus_tag="AC16CY_23630"
     CDS             3109..3984
                     /gene="pstC"
                     /locus_tag="AC16CY_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR3356864.1"
                     /translation="MRKAEFLIEWGLFGCALLSIATTIGIIVVLTIETAAFLEEVPVA
                     DFVFGTEWTPLFAMPRFGVLPLVTGTLLVSVIAMLVALPMGLLSAIYLSEYADERLRR
                     TVKPILEILAGVPTVVYGYFALLFVTPLLQRVIPGMSGANALSPGIVMGLMILPLVSS
                     LSEDAMRGVPRGLREGSYALGATKMQTSLRVVVPAAFSGITAAFILAVSRAIGETMIV
                     AIAAGQQPRLTLDPTVPIETMTAYIVQVSQGDTPQGTLEYRTIFAVGMLLFIGTFGLN
                     LVSAWLRERYREEYA"
     gene            3981..4853
                     /gene="pstA"
                     /locus_tag="AC16CY_23635"
     CDS             3981..4853
                     /gene="pstA"
                     /locus_tag="AC16CY_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006456590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR3356865.1"
                     /translation="MSAAVASGMARRRGADTAFQALALLVLVVTLGALATLIWDIVAD
                     GGGTLSWSFLTSFSSRRASQAGIWHALTGSIFVVLVTAVLAVPVGVAAAIYLEEYGGR
                     SLTARIIELNITNLAAVPSIIYGLLGLGLFVRTMGMGRSVLAGGATLALLALPVVILS
                     TREALRAVPKSLREGSYALGATKWQTIWHQVLPVAAPGILTGLILALSRAIGETAPLI
                     TIGAFAFVTFAPDSIWSPFTVLPIQIFNWVSRPQVEFHANAAAAILVLVTLLLSLNAA
                     AIWLRDRYQKGGIS"
     gene            4940..5686
                     /gene="pstB"
                     /locus_tag="AC16CY_23640"
     CDS             4940..5686
                     /gene="pstB"
                     /locus_tag="AC16CY_23640"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR3356866.1"
                     /translation="MEVDSLNFYYGETRALQDISLQIQPNVVTAFIGPSGCGKSTFLR
                     TLNRLNDIIPGTRVEGRILIDRVDIYRSGMNVVDLRRKVGMVFQKSNPFPKSIFENVA
                     YGLRINGMAGSRSELAGRVEDSLTQAALWDEVKDRLQESALAMSGGQQQRLCIARALA
                     VRPDILLMDEPASALDPIATQRIEELIYDLKKNYTIVIVTHNMQQAARVSDRTAFFWL
                     GRLVEYDRTEKIFTAPTEKLTEDYVTGRFG"
     gene            5686..6366
                     /gene="phoU"
                     /locus_tag="AC16CY_23645"
     CDS             5686..6366
                     /gene="phoU"
                     /locus_tag="AC16CY_23645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR3356867.1"
                     /translation="MDRQDRQVPHFHEELEALQGRLLEMGGLAEERVRAAVQGLVTRD
                     PALMDKVLAGDEPINQLHVEVDNRCFRLLALHQPMATDLRAIVAAVKINTDLERVGDL
                     AVNIAEASMRYLAHAPVKPLIDIPSMGNIAQAMLRDALDAYVRRDTELARRVLNEDDR
                     LDVLKTQVFRELLTLMLQDRSTVEPSLDLILVSRHLERIGDHATNIAEDVIFMVSALD
                     VRHHLTGS"
     gene            6452..6652
                     /locus_tag="AC16CY_23650"
     CDS             6452..6652
                     /locus_tag="AC16CY_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR3356868.1"
                     /translation="MGVIIARMGEAPLCVLCRARPVDEPWRPFCSERCRNEDLARWAE
                     ERYKMAGEPAPDTALDAEADRD"
     gene            6668..7954
                     /locus_tag="AC16CY_23655"
     CDS             6668..7954
                     /locus_tag="AC16CY_23655"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR3356869.1"
                     /translation="MADTLTVTDNRTGKQYEIPIADGAIRAMDLRQIKAGKDDQGLGT
                     YDPAFMNTASCRSRITYIDGDKGILEYRGYPIEQLAERSTYLETAYLIIFGQLPTDSQ
                     LKTWIHAITLHTMLHENIKKLMEGFQYDAHPMSVFLSTVGAFSTFYADAKRIFDEESR
                     KKQIHRLIAKVPTIAAYAYRHSIGRPYNYPDNDLSFTGNFLNMLFRMTELKYQVNPVL
                     ERALEVLFILHADHEQNCSTSAMRGIGSSHVDPYSAMAGAAAALYGPLHGGANEAVLR
                     MLNEIGTVQNIPGFIKRVKAGEGRLMGFGHRVYKSYDPRARIIKQTADQVFEVTGRNP
                     LLDIALELERIALEDEYFISRRLYPNVDFYSGLIYQAMGFPVTMFPVLFAIPRTSGWI
                     AQWEEMLLDPEQKISRPRQLYIGAPRRDYVALDKRG"
     gene            8004..8951
                     /locus_tag="AC16CY_23660"
     CDS             8004..8951
                     /locus_tag="AC16CY_23660"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3356870.1"
                     /translation="MALFFLACNGGAASDATAPPSNSRTVAQSNPGTPAPSIAFDSSK
                     AWEHLRRQVGFGPRPSGSPAILDTRRYIVGELKASGIDVREQPFAGATPLGEVSMANV
                     IGTIPGKRPDRLILASHYDTKLFKEFRFVGANDGASSTAVLLELGRVLKGRQSELTIE
                     LLFLDGEEARMTEWRGTDNTYGSRYYVQNAQKNGNLKTIKALVLLDMIGDKDLVVRRD
                     SNSTPWLVDIIWAAAARLGRGSTFSNELTTIEDDHIPFLRAGVAAADVIDLETPMRRG
                     SWHTPDDTLEFVSARSLQVVGDVVLAAIPEIEKRIVSQR"
     gene            8956..9423
                     /locus_tag="AC16CY_23665"
     CDS             8956..9423
                     /locus_tag="AC16CY_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3356871.1"
                     /translation="MSILNLKVNGRSHTVDVDPATPLLFVLSDDLDLRGPKFGCGLGQ
                     CGSCTVLADGRAIRSCVTPVGTMTNAEIVTLDGLATSNGPHPIQRAFIEQQASQCGFC
                     LSGVILTAKAFLDANPRATDAQIEQALSGVLCRCFTNVRMLEAIRRYAREARA"
     gene            9420..>9777
                     /locus_tag="AC16CY_23670"
     CDS             9420..>9777
                     /locus_tag="AC16CY_23670"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3356872.1"
                     /translation="MTAPLSRDARGALDRAGFSRRDFLKGSGALVVAFGSAAVAHRYG
                     VLAQGINGAGSTQLDSWIAVGSDGAVTAYTGKCDFGQGLFTAQMQLVAEELAVPLSRV
                     RLIQCDTSMTPDQGTTS"
BASE COUNT         1711 a         3309 c         3142 g         1615 t
ORIGIN      
        1 gccgtgtcgc gacgcgcgct ttgtgaatgg cgctgacgtt gcccctccgc gcggacggca
       61 aacttcgatc gagcgccacg aggcgctggc ggatcaacag ctccgaaggt cccgcctcgg
      121 tcatgaacgt cgctcgtctt ggaccgctat gcggctcgtt gcgtgaattt tgtactcgtt
      181 gtgacacgca tagcggtcct tgccgcgcgg agcgcggcaa gagatactgg caaccagtcg
      241 agggctctgc cgggatatca agggcagagc ccttgactgg ttactatagt ccggacccca
      301 cagagaatcg aagcgatgca gatcgccgcg atcgacgtcg gcacaaattc ggttcacatg
      361 atcgtcgtcc gtgtgcgccc cgatctgtcg ttcgaagtcg tcgatcggga aaaggtgatg
      421 gtgcggctcg gcgcgggcgg gctcgacggc aaggcgctga cgagcgaggc gatgagctcg
      481 tcgctgcagg cgctctcgaa gttcaagcgc ctcgccgaat cgcacggcgt ggacgtcatc
      541 ctcgcagccg cgacgagcgc gacgcgagag gcgcggaacg gcggcgagtt cctcgcgcgc
      601 atcgaggccg agaccggcat caggccgcgc gtgatcacag gggccgaaga ggcgcggctg
      661 atccatcagg ccgcggtgta cggcgtggac gtcggcagcg cgcgggccgt cgtgatcgac
      721 atcgggggcg gcagcgtcga gatcacacac ggcaccgcca cccagattca ggcggcccgc
      781 agcttcaaga tcggcgtcat ccgcctgacc gagcggttca tccacagcga tcccctctcg
      841 gggcgcgacg aagagaagct gaccaggcac gtgctgaacg agatcgaccg gcactgcgag
      901 caaatcaccg ccgcaggctt cgatcgcgtc atcgggactt ccggcacgat catgagcctg
      961 ggagccgtgg ccgtggccct cacgacgggg acaccgccga gcgaactgcg gaacctgcgc
     1021 gtgtcggcaa accagatccg ccggcttcgc aaggagatcg tcgggctttc accagaacag
     1081 cggctggcgc ttgccggtct cgaccctcgc cgcgcggacc tggccgtcgc cggcgcgttg
     1141 ctgctcgatt tgatcctccg gcggctcgac gccgaaagcc tcacgctgtg cgatctggcc
     1201 ctgcgcgaag ggctggtgct cgactacatc cgccgcaaca gcaaacagat cgcgcgagcc
     1261 gaacgcatcc ccgacgtgcg ccgccgcagc acgattgagc tggcggagcg ctgcagctac
     1321 tacgccgagc actcgcagca ggtcgcgcgg ctggcgctgg cgatcttcga tcagacacga
     1381 gccgtccacg ggctcaccga ccgcgagcgc gagtggctgg agtttgcgtc gctgcttcac
     1441 gacatcggca gcctcatcag cttttcacgc caccatcgcc actcctacta cctgattcgc
     1501 aacggcggcc tgcggggctt tcagccggac gagatcgagg tgatggcgct ggtcgcccgg
     1561 taccaccgcc gcggcacgcc gaagcggtcc cacgacgagt acgcgcggct gccggctccc
     1621 ctgcgccgca tcgtgcggat gctgtcgtcc attctccggg ttgccgagtg cctcgaccgg
     1681 agccacacgc aggccatctc gggcctggac ctgcgcgatc gcggcaagga cgtgctgata
     1741 caggtgcaca cctcggcgga tgccgagctg gaaatctggg ccaccaaccg ccacctgaaa
     1801 ccgttcgaga aactcgtcgg caagcccgtg catctcgaag cggccgccgt cccctccgtt
     1861 gcggttcccg aacgcgcctc cgccgagaag gtcaagaaga cggccggtca ctcgcgcggc
     1921 aatggccact cccggccccg aaaagtggtc gtcagccccc ggcagcccgc gcctgcgagc
     1981 agccgcgctc ccgaacgccg aacctagcta ccggccaccg gctacccgcc gcgttacacg
     2041 gatttaacac cccagaaacc agtgtgaaat tcgcgttcga taacttcagc tcatgctgat
     2101 ggttatccgt gtctccgggc tcgttctcgc ggtcgtctcc cttgccgcct gcagcggcgg
     2161 gtcgaacgcc ggtgagtcgg gcgctccggc catgattcag atcgacggct cgagcacggt
     2221 gtttccgatc accgaggcgg tcgccgaaga gtttcaaaag accgataccg ccgtgcgcgt
     2281 gacggtcggc atctcgggca cgggcggcgg cttccagaag ttctgccgcg gcgagaccga
     2341 catcagcaac gcgtcacggc cgatccgtcc gaccgaaata gagacctgtt cgaaggcggg
     2401 catcgagtac atcgagctgc ccgtcgccta cgacggcctc gccatcgtcg tcaacccgaa
     2461 gaacacgtgg gcggatcagg ttgccgtgtc cgagctgaag acgctctggg cgcccgaggc
     2521 gcagggcaag atcctccggt ggaaccaggt gcgtggttcc tggccggacc gcgagatccg
     2581 gctgttcggc gctggcgtcg attcgggcac gtacgactac ttcaccgaag ccacggttgg
     2641 aaaagaggga accagccggg gcgacttcac ctccagcgaa gacgacaacg tgctcgtcca
     2701 gaacatcagt accgacgagc tcgccctcgg attcctgccg ctggcgtatg tcgaacagaa
     2761 ccagtcgaag ctgaagctcg tgccggttga cgacggcaag ccggacaacg gaaagggccc
     2821 gatcacgccg acggccgcca cggttcgcga cggcacgtat caacccctct cccgcccctt
     2881 gttcatctac gtcgcgcgca aggcggcgga tcgcccggag gtccagcgct tcgtccaggc
     2941 gtacctcgac tcggcgcccc tgatgcggga agtcggctac gtggagctga cgccgcagat
     3001 ctacgacctc gcgcgaaagc acttctccga tcgacgagtt ggcacggctt tcggtgcagg
     3061 cgggtcgcag gtaggagtga ccctcgagca actgctgacc cgggagcaat gaggaaagct
     3121 gagttcctca tcgaatgggg cctcttcggg tgcgcgctcc tgtccatcgc caccacgatc
     3181 ggcatcatcg tcgtcctcac catcgagact gccgcttttc tcgaggaagt gccggtcgcc
     3241 gacttcgtgt tcggcaccga atggactccc ctgtttgcga tgccccgctt cggggtgctc
     3301 ccgctcgtca cgggcacgct gctcgtgtcg gtcatcgcca tgctcgtggc tctcccgatg
     3361 ggcctgctga gcgcgatcta cctcagtgag tacgccgacg agcgcctcag gcggaccgtc
     3421 aagccgattc tcgagattct ggcgggcgtg ccgacggtcg tctacggcta cttcgcgctg
     3481 ctcttcgtca cgccgctctt gcagcgcgtc atccccggga tgagcggcgc caatgccctc
     3541 agcccgggca tcgtcatggg tctcatgatt cttccgctcg tgtcctctct atcggaggac
     3601 gccatgcgtg gcgtgccgcg cgggctgcgg gagggatcgt acgcgctcgg cgcgacgaag
     3661 atgcagacga gcctgcgcgt ggtcgtgccg gccgcctttt cgggcattac cgccgcgttc
     3721 atcctcgccg tgtcgcgcgc gatcggggag acgatgatcg tggcgatcgc cgccgggcag
     3781 cagccacggt tgacgctcga cccgacggtc ccgatcgaga cgatgaccgc ctacatcgtg
     3841 caggtgagcc agggcgatac gccgcagggc acgctcgagt accgcacgat cttcgccgtg
     3901 ggaatgctgc tcttcatcgg cacgttcgga ctgaacctcg tcagcgcatg gctgcgcgag
     3961 cggtaccggg aggagtacgc gtgagcgccg ctgttgccag cggcatggcg cgccgccgcg
     4021 gcgcggacac cgccttccag gcgctggcgc tcctggtcct cgtggtcacg cttggcgctc
     4081 tcgccacgct catctgggac atcgtcgccg acggaggggg cacgctgtcg tggagcttcc
     4141 tcacgagctt ctcgtcgagg cgcgcctcgc aggccggcat ctggcatgcg ctcaccggca
     4201 gtatcttcgt tgtcctcgtc acggccgtgc tcgcggtgcc ggtcggcgtg gccgccgcga
     4261 tctacctcga ggaatacggc ggccgcagcc tgactgcccg catcatcgag ttgaacatca
     4321 ccaacctcgc ggccgtcccg tcgatcatct acgggctgct gggcctgggg ctgttcgttc
     4381 gaacgatggg gatggggcga agcgtcctgg ctggtggcgc gacgctcgcg ctgctggcct
     4441 tgccggttgt catcctgtca acgcgagagg cgttgagagc cgtacccaag tcgcttcgcg
     4501 agggctcgta cgcgctcgga gcgaccaagt ggcagacgat atggcaccag gtgctccccg
     4561 tcgccgcgcc gggcatcctt accggcctca tcctggccct ctctcgcgca atcggggaga
     4621 cagcaccgct catcacgatc ggcgccttcg cgttcgtgac cttcgcgccc gactcgatct
     4681 ggtctccgtt cacggtcctg ccgattcaga tcttcaactg ggtgtcccgc ccgcaggtcg
     4741 agttccatgc caacgcggcg gcagcgattc tggtccttgt cacgcttctg ctctccttga
     4801 acgccgcggc aatctggctc cgggaccggt accagaaggg ggggatctcg tgagcgccgt
     4861 gccgcggtcc ctgccgagcc tgggcgaggt ggcgtcgtcg cggcgcgagg ccgccggggg
     4921 cgccgacagg gcgtgcaaga tcgaggtgga ctcgctgaac ttctactacg gcgagacgcg
     4981 ggcgctccag gacatctcac tccagatcca gccgaacgtg gtcacggcat tcatcgggcc
     5041 gtcgggctgc ggcaagagca cgttcctgcg cacgctgaac aggctgaacg acatcatccc
     5101 cggcacgcgc gtcgagggcc gcatcctcat cgacagggta gacatctacc ggtccggcat
     5161 gaacgtcgtg gatctgcgcc gcaaggtcgg catggtcttc cagaagtcga atccgtttcc
     5221 gaagtcgatc ttcgagaacg tcgcctacgg cctgcgcatc aacgggatgg ccggcagccg
     5281 gtcggagctg gccgggcgcg tcgaggacag cctcacgcag gccgccctct gggacgaagt
     5341 gaaggaccgg ctccaggaat cggcgctcgc catgtcggga ggccagcagc agcggctctg
     5401 catcgcgcgt gccctcgccg tgcgtcccga catcctcctg atggacgagc ccgcgtcggc
     5461 gctcgacccg attgccaccc aacggatcga ggaactcatc tacgatctga aaaagaacta
     5521 cacaatcgtg atcgtgacgc acaacatgca gcaggcggcg cgcgtgtcgg accggacagc
     5581 gttcttctgg ctcggccggc tggtcgagta cgaccggacc gaaaagatct tcacggcgcc
     5641 gaccgaaaag ctgacagaag actacgtgac cgggagattc ggctgatgga ccgacaggac
     5701 cggcaggtcc cgcactttca cgaggagctc gaagcgctcc agggacggct gctcgagatg
     5761 ggcggcctgg cggaggagcg cgtccgcgcc gccgtgcagg ggctcgtgac gcgcgacccc
     5821 gcgctgatgg acaaggtcct cgccggcgac gagccgatca accagctgca cgtcgaggtg
     5881 gacaaccgct gcttccggct gctggcgctg catcagccga tggccaccga tcttcgcgcc
     5941 atcgtggccg cggtcaagat caataccgac ctcgagcgcg tcggcgacct ggccgtgaac
     6001 atcgccgaag ccagcatgcg ctatctggcg cacgcgccgg tgaagccgct catcgacatc
     6061 cccagcatgg gcaacatcgc ccaggcgatg ctgcgcgacg cgctcgacgc gtacgtcagg
     6121 cgcgacaccg agctggcccg ccgggtcctc aacgaggacg atcgactgga cgtcctgaag
     6181 acgcaagtgt tccgcgaact gctcaccctt atgctgcagg atcgatcgac ggtcgagccg
     6241 tcgctcgatc tcatcctcgt ctctcggcac ctcgaacgca tcggcgacca cgcgaccaac
     6301 atcgccgaag acgtcatctt catggtctcg gcgctcgacg tgcggcacca cctgacgggc
     6361 agttgatcga atcactcccc attcccgatt cccaactccc aaccggggct gtacggacgc
     6421 acagaattgt ttgagcgttg ggaattggga gatgggagtt atcattgccc ggatgggcga
     6481 ggcaccattg tgcgtcctgt gccgcgctcg tccagtagat gaaccctggc gtccgttctg
     6541 cagcgagcga tgccggaacg aggacctggc tcgatgggcg gaggagcgct acaagatggc
     6601 cggtgagccg gcccccgata cggcgctcga tgccgaggcc gaccgcgact gatacctgtt
     6661 ccttcttatg gccgacacgc tcaccgtcac cgacaaccga actggaaaac aatacgagat
     6721 ccccattgcc gacggcgcga tccgcgcgat ggacctgcgt cagatcaaag ccggaaagga
     6781 tgaccagggc ctcggcacct acgacccggc gttcatgaac acggcctcct gccggagccg
     6841 catcacctac atcgacggcg acaagggcat cctcgagtac cgcggctatc cgatcgagca
     6901 gctggctgag cgctccacct atctggagac ggcgtacctc atcatcttcg gtcaactgcc
     6961 gaccgactcg cagctgaaga cgtggatcca cgcaatcacg cttcacacga tgctgcacga
     7021 gaacatcaag aagctgatgg aggggttcca gtacgacgcc cacccgatga gcgtgttcct
     7081 gagcacggtc ggcgcgtttt cgaccttcta cgccgacgca aagcggatct tcgacgagga
     7141 gtcgcgcaag aagcagatcc accgcctcat cgcaaaggtt ccgaccatcg ccgcctacgc
     7201 gtaccggcac agcatcgggc gcccctacaa ctacccggac aacgatctca gcttcacggg
     7261 caacttcctc aacatgctgt tccggatgac cgaactgaaa taccaggtca acccggtgct
     7321 cgagcgggcg ctcgaggtgc tgttcatcct ccacgccgac cacgagcaga actgcagcac
     7381 gagcgccatg cgcgggatcg gcagctcgca cgtggatccg tactcggcga tggcgggggc
     7441 cgcggccgcg ctgtacggcc cgctgcacgg cggcgccaac gaggccgtgc tgcgcatgct
     7501 caacgagatc ggcaccgtgc agaacatccc gggattcatc aagcgggtca aggccggcga
     7561 ggggcggctg atgggattcg gccaccgggt ctacaagtcg tacgaccccc gggccaggat
     7621 catcaagcag acggccgacc aggtcttcga ggtgacaggg cgaaatccgc tgctcgatat
     7681 cgcgctcgag ctcgagcgga tcgcgctcga ggatgagtac ttcatctccc ggcggctcta
     7741 tcccaacgtg gacttctact ccggcctgat ttaccaggcg atgggcttcc cggtgacgat
     7801 gttcccggtc ctgttcgcga ttccgcgcac gtcaggatgg atcgcgcagt gggaggagat
     7861 gctgctcgat ccggagcaga agatctcgag gccgcgccag ctctacatcg gggcaccgcg
     7921 ccgggattac gtggcactgg ataaacgggg gtaggacggt gcgagggcgc gagggtgcga
     7981 cggtgcgaag gtgcgtggct gcgatggccc tattcttcct tgcctgtaac ggcggcgcgg
     8041 cgagcgacgc cactgcccca ccttcgaatt ctcgcaccgt cgcacaatcg aaccctggca
     8101 cccccgcacc gtcgatcgcc tttgacagca gcaaagcctg ggaacacctt cgccgtcagg
     8161 tcggcttcgg cccgcgccca tcgggctcgc cagccatcct cgacacgcgt cgctacatcg
     8221 tcggcgagct caaggcgtcc ggcatcgacg tgcgcgagca gccgtttgcc ggcgcgacgc
     8281 cgctcggcga ggtctcgatg gcgaacgtca tcggcaccat cccgggcaag cggcccgatc
     8341 gtctgatcct ggccagtcac tacgacacga aactcttcaa ggaattccgg ttcgtcggcg
     8401 ccaatgacgg cgcgtcgtcc acggcggtcc tgctcgagct gggccgtgtc ctcaagggac
     8461 ggcagagcga gctcacgatc gagctgctct tcctcgacgg cgaggaagcg cggatgacgg
     8521 aatggcgcgg cacggacaac acctacggca gccggtacta cgtccagaac gcccagaaga
     8581 acggcaactt gaagacgatc aaggcgctgg tgctgctcga catgatcgga gacaaggatc
     8641 tcgtcgtccg gcgcgactcc aattccactc cgtggctcgt ggacatcatc tgggccgccg
     8701 ccgcgcgact cggccgcggc tccaccttct ccaacgagct gacgacgatc gaggacgacc
     8761 acataccgtt tctgagggca ggcgtcgccg ccgcagacgt gatcgacctg gagacgccga
     8821 tgcggcgcgg cagctggcac actcccgacg acacgctgga gttcgtgagc gcccgtagcc
     8881 tgcaggtggt cggcgacgtg gtcctcgccg ccataccgga gatcgaaaag aggatcgttt
     8941 ctcaacggta ggcccatgag catcctgaac ctgaaggtga acggacgctc gcacacggtg
     9001 gacgtggacc cggccacgcc gctgctgttc gtcctgagcg acgacctcga cctgcgcggg
     9061 cccaagttcg gctgcggcct cgggcaatgc ggctcgtgca cggtgctcgc cgacgggcgg
     9121 gcgatcagat cgtgcgtcac gcccgtcggc accatgacga acgccgagat cgtcacgctc
     9181 gacgggctcg ccacctccaa cgggccgcat ccgattcagc gcgcgttcat cgagcagcag
     9241 gcgtcgcagt gcggcttctg cctgagcggc gtcatcctga ccgcgaaggc cttcctcgac
     9301 gcgaatcccc gcgcgaccga cgcgcagatc gagcaggcgc tctcgggggt gctctgccgc
     9361 tgcttcacca acgtccggat gctcgaggcc atccggcgct acgccaggga ggccagggca
     9421 tgacggctcc gctctcccgg gacgctcgag gcgcgctcga tcgcgcgggc ttctcgcgcc
     9481 gcgatttcct caaaggatcc ggcgccctcg tcgtcgcgtt cgggtcggcg gcggtggcgc
     9541 accggtacgg cgtcctcgcg caaggcatca acggcgcggg cagcacgcag ctcgattcgt
     9601 ggattgccgt cggcagcgac ggagccgtca ccgcctacac gggcaagtgc gatttcggac
     9661 aagggctctt caccgcccag atgcagctcg tcgccgaaga gctcgcggtc ccgctcagcc
     9721 gcgtccgtct gattcagtgc gacacctcga tgacgcccga tcaaggcacg acctccg
//
LOCUS       DBNGSH010001044         3500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4236527, whole genome shotgun sequence.
ACCESSION   DBNGSH010001044 DBNGSH010000000
VERSION     DBNGSH010001044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4236527"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2596)
                     /locus_tag="AC16CY_23675"
     CDS             complement(<1..2596)
                     /locus_tag="AC16CY_23675"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3356873.1"
                     /translation="MNLSALFIARPVTTTLLMLGILVFGGLAYRELPVADLPSVDFPS
                     IRVSANLPGASAETVASTVALPLEKQFATISGLTSINSTSTQGGTDIQLQFDLSRDID
                     AAAQDVQAMIGRASRQLPPQMPAPPSYQKVNPSDQSIFFVVLRSKTLPLSTLDEYAQS
                     NVAQRISTVSGVAQVNVFGSQKYAVRVDVDPRELAARSIGIDEVATAIESANANLPTG
                     SIYGEKTFTVETNGQLLRGAAYGPTIIAYRNGNPVRLDEVAHVYDGVENDRTASWQNG
                     ERCVYLSIQKQPGVNVVETVERIHALLPGIRAQLPAAISLDVRSDRSVTIRESVHDVK
                     VTLVVTMCLVVLVIFLFLRNVSATIIPSLALPGAVVGTFAVMYLLGYSVDNLSLMALT
                     LSVGFVVDDAIVMLENIVRHMEMGKPRMRAALDGSKEIGFTILSMTASLVAVFIPVLF
                     MGGIVGRLLREFAVTITAAILLSGFVSISLTPMLCSRFLRPPHADGHSRLYNAFERLF
                     EGWRNVYGWTLSATLRHRTITVAVSVALLAASGYLFVVTPKGFLPSEDQGRFNVNTEG
                     AQGISFDQMVRHQLRVADVLLEDPSVASVGVNVGLIGNSGSGGSNTGRMFVEMKPRAE
                     RSLTVDQVIAALRPKFEQIPGVRVFMVNQLPINLGGGGGSRGLYQFSMQDTDTDELYR
                     WAPVMEQAIRQSPGFEDVSSDLQINNPLVTVEMDRDKMSRLGLTATQVETALFNAYGT
                     RQVSQIYAPNNQYQVILRVAPEFQSDPAAMSLLHVRSNSGRLIPLESVARLSTAVGPF
                     QVNHFSQLPSVTVSFNLAPGYALGDAVERIQSTAAQTLPASIILAFQGTAQAFQDSLR
                     GLGLVLLMA"
     gene            complement(2593..>3500)
                     /locus_tag="AC16CY_23680"
     CDS             complement(2593..>3500)
                     /locus_tag="AC16CY_23680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3356874.1"
                     /translation="EGDDVKAGQVLFTLDKRPLEAGLEQAKANLDRDLAQAANAEAQF
                     KRYQELADRGIAPKEQVDTARASMSALNATVNADRAALENAQVQLQYATITATLSGRT
                     GELQVNEGNLVRANDQTPLVVINQIAPIFVTFAIPESRLPELKRYMARGPLRVTASPP
                     KDDGPPAVGRISFVDNQVDQTTGTIKLKGTFPNDDRRLWPGQFLNLLVTLTSEPDAIV
                     VPTAAVQAGQQGPYVFVVKDDRTVDMRAVDVSRTRASETVIASGVTPGETVVTDGHLR
                     LVPGSRISVKSEERGPAAPAGAGAS"
BASE COUNT          551 a         1173 c         1190 g          586 t
ORIGIN      
        1 ttgccatcaa cagcacgagc cccaggcctc gcagcgagtc ctggaacgcc tgcgcggtcc
       61 cctgaaacgc caggatgatc gacgccggca aggtctgcgc ggccgtggac tggatgcgct
      121 cgaccgcgtc gccgagcgcg tagcccggcg caaggttgaa cgacacggtc accgacggaa
      181 gctggctgaa gtggttcacc tggaacgggc cgacggcggt gctgagccgc gcaaccgatt
      241 cgagcggaat caggcgtccg ctattcgatc ggacgtgcag cagcgacatc gccgccgggt
      301 cgctctgaaa ctcgggagcg acgcgcagga tcacctgata ctgattgttc ggcgcgtaga
      361 tctgcgagac ctgtctcgtg ccgtacgcgt tgaagagcgc ggtttccacc tgcgtggccg
      421 tcaggcccag cctcgacatc ttgtcgcggt ccatctcgac ggtgaccagc gggttgttga
      481 tctgcaggtc gctgctgacg tcctcgaagc cggggctctg ccgaatcgcc tgctccatga
      541 ccggcgccca cctgtacagt tcgtcggtgt cggtgtcctg catcgagaac tggtacagcc
      601 cacggctccc gccgccgccg ccaaggttga tcggcagctg gttgaccatg aagacgcgta
      661 ccccgggaat ctgctcgaac ttcgggcgca gcgccgcgat gacctgatcg acggtcagcg
      721 agcgttcggc gcgcggcttc atctcgacga acatccggcc ggtattggag ccgccggagc
      781 cgctgttgcc gatcaggccg acgttcacgc cgacgctggc gacgctcgga tcctcgagca
      841 ggacgtcggc cacccgtagc tggtgccgca ccatctggtc gaagctgatg ccctgcgcgc
      901 cctcggtgtt gacgttgaag cggccctgat cctcgctggg cagaaacccc ttcggcgtca
      961 ccacgaacag gtagccgctg gccgcgagca gcgccaccga cacggccacg gtgatcgtcc
     1021 gatggcggag ggtcgcgctc agcgtccagc cgtagacgtt ccgccaccct tcgaacagcc
     1081 gctcgaacgc gttgtagagc cggctgtgcc cgtccgcgtg cggcggcctc aggaagcggc
     1141 tgcacagcat cggggtcagg ctgatcgaca cgaaccccga caacaggatg gcggcggtaa
     1201 tcgtcaccgc gaactcgcgc agcaggcgcc cgacgatgcc gcccatgaag agcaccggga
     1261 tgaagaccgc gacgagcgac gcggtcatcg acaggatcgt gaagccgatc tccttcgacc
     1321 cgtccagcgc ggcgcgcatc cgcggcttcc ccatctccat gtgcctgacg atgttctcca
     1381 gcatcacgat cgcgtcgtcc acgacgaagc cgaccgagag cgtgagcgcc atcagcgaca
     1441 ggttgtccac gctgtagccg agcaggtaca tgaccgcgaa cgtgccgacg accgcgccgg
     1501 gcagggccag gctgggaatg atcgtcgccg acacgttgcg cagaaagagg aagatcacga
     1561 gcacgaccag gcacatcgtg acgacgagcg tcaccttcac gtcgtggacc gactcgcgga
     1621 tcgtgaccga ccggtcgctg cgcacgtcga gcgagatcgc cgccggcagc tgcgcgcgaa
     1681 tgcccgggag cagcgcatgg attcgttcga ccgtctcgac gacgttgacg ccgggctgct
     1741 tctgaatcga caggtagacg caccgctcgc cgttctgcca gctggccgtc ctgtcgttct
     1801 cgacgccgtc gtacacgtgc gcgacctcgt ccaggcgcac cgggttgccg ttgcggtacg
     1861 cgatgatcgt cggcccgtag gcggcgccgc gcagcagttg gccgttggtt tccaccgtga
     1921 aggtcttctc gccgtagatc gagccggtcg gcaggttcgc gttggcgctc tcgatcgccg
     1981 tcgccacctc gtcgatgccg atcgagcgcg ccgccagctc cctgggatcg acgtccacgc
     2041 gcacggcgta cttctgcgag ccgaagacgt tcacctgggc gacgccgctg accgtcgaga
     2101 tgcgctgggc gacgttggac tgcgcgtact cgtccagggt cgacagcggc agcgttttcg
     2161 accggagcac gacgaagaag atcgactggt ccgacggatt cactttctga tacgagggcg
     2221 gcgccggcat ctggggcggg agctgccgtg acgcgcggcc gatcatcgcc tgcacgtcct
     2281 gcgcggcggc gtcgatgtcg cggctcaagt cgaactgcag ctggatgtcg gtgccgccct
     2341 gggtgctggt cgagttgatg ctggtgagcc cggagatggt ggcgaactgc ttctcgagcg
     2401 gcagggcgac cgtggaggcg accgtctcgg cgctggcccc gggcagattg gcgctgacgc
     2461 gaatggacgg gaagtccacg ctcggaaggt cggcgacggg cagctcccgg tacgcgagcc
     2521 ctccgaacac gaggatgccc agcatgagca gcgtggtcgt caccgggcgc gcgatgaaga
     2581 gcgccgagag attcatgagg ctccggcccc ggctggcgcg gcgggccctc gttcctcgct
     2641 cttcacgctg atgcggctgc cgggcacgag ccgcaggtgg ccgtcggtga cgacggtctc
     2701 cccgggtgtc acgccgctgg cgatcaccgt ctcgctcgcg cgcgtgcggc tgacgtccac
     2761 cgcccgcatg tcgaccgtgc gatcgtcctt taccacgaac acgtacggcc cctgctgccc
     2821 cgcttgaacg gccgcggtcg gcacgacgat cgcatccggt tcgctcgtca gcgtcaccag
     2881 gaggttgaga aactgccccg gccagagacg ccgatcgtcg ttcggaaagg tgcctttcag
     2941 cttgatcgtg ccggtcgtct ggtcgacctg gttgtccacg aacgaaatcc tgccgacggc
     3001 gggcggcccg tcgtctttgg gcggcgaggc ggtgacgcgc agcgggccgc gcgccatgta
     3061 ccgcttcagc tcgggcaggc gcgactccgg gatcgcgaac gtgacgaaga tcggggcgat
     3121 ctggttgatg accacgagcg gcgtctgatc gttggcgcga acgagattac cctcgttcac
     3181 ctgcagctcg ccggtccggc ccgagagcgt ggcggtgatc gtggcgtatt gcagctgcac
     3241 ctgcgcgttc tcgagcgcgg cgcgatccgc attcaccgtg gcgttgagcg cgctcatcga
     3301 ggcgcgggcg gtgtcgacct gctccttcgg cgcgatgccg cgatcggcga gctcctggta
     3361 gcgcttgaac tgcgcctcgg cgttggccgc ctgcgcgagg tcgcgatcga gattcgcctt
     3421 cgcctgctcc agcccggcct cgagcggccg cttgtcgagc gtgaagagca cctgcccggc
     3481 cttgacgtcg tcgccttccg
//
LOCUS       DBNGSH010001045         6061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2028915, whole genome shotgun sequence.
ACCESSION   DBNGSH010001045 DBNGSH010000000
VERSION     DBNGSH010001045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2028915"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="AC16CY_23685"
     CDS             complement(<1..770)
                     /locus_tag="AC16CY_23685"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3356875.1"
                     /translation="MTAPIVDPMVGRTIAQYDILARVGGGGMGVVYRARDAKLGRTVA
                     LKFLPQQWSHDEAAKQRFLREAQAASATNHPNICTIHDIETAADGQLFIVMAYYEGQT
                     LKQRLEQGPLSVDEALDIATQLADGLAKAHAQGVVHRDVKPGNVMLTEDGVRILDFGL
                     ATFADALKLTIEHSTLGTAAYMSPEQVQGRAADPRSDVWAAGVVLYEMLAGHVPFQGS
                     HAEAVSYAIRNETPAPLRSSRPGIPEEVEQLVFRALHK"
     gene            complement(828..1814)
                     /locus_tag="AC16CY_23690"
     CDS             complement(828..1814)
                     /locus_tag="AC16CY_23690"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR3356876.1"
                     /translation="MICVEITRPGEPEVLVPAERPDPAPGPDDVLIRVAGAGVNWPDV
                     LQRRGLYPPPPGASDIPGLEVSGTVAAVGARVEQWRVDDPVVALVSGGGYATLCVAPA
                     RQCLPVPASIDLVSAAAIPEAFFTVWTNVFDRGRLRAGETALFHGGSSGIGTTAIQLA
                     AARGATVLATAGTDEKCRACEEIGATQAINYRTADFVEAVRQLTDGRGVDLILDIVGG
                     GYVARNLQCLAMDGRLVQIGFMSGQSDATVDFRRVLSRRLTITGSALRPRTIEQKGEI
                     AMALRREVWPLIDRGQVKPIVHRVFPLSEAADAHRLMESSSHVGKIVLTMRS"
     gene            complement(1883..3103)
                     /locus_tag="AC16CY_23695"
     CDS             complement(1883..3103)
                     /locus_tag="AC16CY_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356877.1"
                     /translation="MNALAERYVKLVLALGQHDADYVDAYYGPPAWRKEAETARRELA
                     DIDREASDVLAALDAARPASGAEELVRLRHQYVTRQLQSLRARVSMLRGEKLRFDEES
                     KALYDAVAPTHAEAEFESILKDLEGRLPGRGSLVDRLDAFRARFVIPKERLDATFKAA
                     IDGCRQRTLERMSLPPEESFAVEYVTDKSWSAYNWYQGGYRSLIQVNTDLPVHVDRAI
                     DLACHEGYPGHHVYNALLEKTLVRDRGWVEYSVYPLFSPQSLIAEGTANYGIDVAFPG
                     PERLAFERKVIFPAAGLDPATVEEYFGVQALVDRLSYAGNEAARRYLDGGIDATAAAE
                     WLGRFGMYTPPRAKQRIRFIDQYRSYVINYNLGKDMVARYVESQAGGTSDRARRWQAF
                     ERLLSSPRLPSGLN"
     gene            complement(3235..4245)
                     /locus_tag="AC16CY_23700"
     CDS             complement(3235..4245)
                     /locus_tag="AC16CY_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3356878.1"
                     /translation="MRTPVTLVTGASGEIGHGLITRLAGTGRPIVTVDLVPLDPALGG
                     LVLREFTGSITDANLLERVLAEFEVDLIFHLAALLSTRSEFTPVTAHHVNVDGTLTLL
                     EFAQREGESHGRTVLFLYPSSVAAYGLPDLAAKSAAGRVREDEWNTPRTMYGCNKLYC
                     EHLGRYYARYYKQLAAHATGRVDFRSVRFPGLISAVTLPSGGTSDYAPEMIHAAARGE
                     PYTCFVRPDTRIPFMAMPDAISALLALADAPREALTRTAYNLTAFSATAQDIHDAVVA
                     AFPKAVVRWATDLKRQTVVDSWPEDVDDSAARRDWGFAPAYDFRGAFEHYVIPKVRER
                     YR"
     gene            complement(4311..5114)
                     /locus_tag="AC16CY_23705"
     CDS             complement(4311..5114)
                     /locus_tag="AC16CY_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIR3356879.1"
                     /translation="MADMHEIDYKIYGDDMQYVEVELDPNEATVAEAGGMMFMDDGIE
                     METVFGDGSRQQKSGFMDALMGAGKRLLTGESLFMTVFFNRTTRRRKVAFGAPYPGKI
                     VPVHLSEIGGTLITQKDSFLAAAKGVSIGIAFQKRLGVGLFGGEGFIMQKLEGDGLCF
                     VHAGGTVHPLDLGAGQTMRVDTGCLVALQPSVAYDIQYVGKIKTALFGGEGLFYAVLT
                     GPGRVWLQSLPFSRMADRIYKAAPAAGGSRKEGGSILGGLGDLLDGDRT"
     gene            complement(5366..5833)
                     /locus_tag="AC16CY_23710"
     CDS             complement(5366..5833)
                     /locus_tag="AC16CY_23710"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3356880.1"
                     /translation="MPIAPRRYLLKVSVGQETYDNLQRVRGLLRHTHPDGDLEAILDQ
                     ALALLLRDTERRRIAAASKPRLAAAAAAPERRYVPAAVRRAVWARDSGQCAFVGPAGR
                     CGETAFLEFHHVVPFSAGGRTDSTNLELRCRAHNHYEARKAGLPIPTRRAASR"
BASE COUNT          959 a         2105 c         1981 g         1016 t
ORIGIN      
        1 tccttgtgca gggcacggaa gacgagctgc tcgacttcct ccgggatccc cggccgcgac
       61 gagcgcagtg gcgccggtgt ctcgttccgg atggcgtacg aaacagcctc ggcgtgcgag
      121 ccctgaaagg gcacatggcc ggcgagcatc tcgtacagca ccacgccggc ggcccagacg
      181 tcggaccgcg ggtcggccgc gcgcccttgc acctgctcgg gcgacatgta cgcggcggtg
      241 ccgagcgtgg agtgctcgat cgtcagcttg agcgcgtcgg cgaacgtcgc gagcccgaag
      301 tcgagaatcc ggacgccgtc ctcggtcagc atcacgttgc ccggcttgac gtccctgtgc
      361 acgacgccct gggcgtgggc ctttgcgagg ccgtcggcca actgcgtcgc gatgtcgagc
      421 gcctcgtcca cgctcagcgg tccttgctcg aggcgctgct tcagcgtctg cccttcgtag
      481 taggccatca cgatgaacag ctgcccgtcg gcggcggtct cgatgtcgtg aatcgtgcag
      541 atgttcgggt ggttggtggc cgacgccgcc tgggcctcgc ggaggaaccg ctgcttggcg
      601 gcctcgtcgt ggctccactg ctgaggcagg aatttcaggg cgacggtccg tccgagcttt
      661 gcgtccctgg ctcgatagac cacgcccatc cccccgccgc cgacgcgggc gaggatgtcg
      721 tactgggcga tcgtgcgccc gaccatcgga tcgacgatgg gagctgtcac cgtggtgtcc
      781 gccgattcgt cccggccctt acaccgggat ggtgagcgcc gattttctca cgatcgcatc
      841 gtcagcacga tcttcccgac gtgcgaactc gactccatca ggcggtgcgc gtcggccgcc
      901 tcggacagcg ggaacacgcg atggacgatc ggcttcacct ggccgcggtc gatgaggggc
      961 cagacctcgc ggcgaagggc catggcgatc tcgcccttct gctcgatcgt tctcggcctc
     1021 agcgcggacc cggtgatggt caggcggcgg ctgagcaccc gccggaaatc gaccgtggcg
     1081 tccgactgtc cgctcatgaa cccgatctgc accagccggc cgtccatcgc caggcactgg
     1141 aggttcctgg ccacatatcc gccgccgacg atgtcgagga tgagatcgac gccccttcca
     1201 tcggtgagct gacggacggc ttccacgaaa tcggccgtgc ggtagttgat cgcctgcgtg
     1261 gcgccgatct cctcgcaggc ccggcatttc tcgtctgtcc ccgcagtggc cagcacggtt
     1321 gctccgcgcg cggcggcgag ctgaatggcg gttgtgccga tgccgctcga cccgccgtgg
     1381 aagagcgccg tctcgcccgc ccgcaggcgg ccgcgatcga acacgttggt ccagaccgtg
     1441 aagaaggctt ccggaatcgc cgcggccgac accaggtcga tgctggccgg caccgggagg
     1501 cactgccgcg ccggagccac gcacagggtc gcgtagccgc cgccggagac gagcgcgaca
     1561 acaggatcgt caactcgcca ctgttcgacg cgcgcgccaa ccgctgcgac ggtgcccgat
     1621 acctcgaggc cgggaatgtc gcttgccccc ggaggcggcg gatacaatcc tcggcgctgc
     1681 aggacgtcag gccagttcac gcctgccccg gccacacgta tcaggacgtc gtccgggccc
     1741 ggtgccggat cgggccgctc cgcaggcacg agcacttctg gctcgccggg acgagtgatt
     1801 tcgacgcaga tcacgctgcc ccctgccccc tgtccccttg ccccctgccc cctgccccct
     1861 gccccctgcc ccctgccccc tgttagttca gccctgaggg aagacggggc gaggacaaca
     1921 gccgttcgaa tgcctgccag cggcgcgccc ggtcggacgt gccgccagcc tgggactcga
     1981 cgtaccgcgc caccatgtcc ttcccaaggt tgtagttgat gacgtagctc cggtactgat
     2041 cgatgaagcg gatgcgctgc ttcgcgcgcg gcggcgtgta catgccgaaa cgcccaagcc
     2101 actcggctgc cgcggtggca tcgatcccgc cgtcgaggta gcgccgcgcc gcttcattgc
     2161 ccgcatacga aagtcgatcg acgagcgcct gcacgccgaa atactcctct acggtggcag
     2221 ggtcgaggcc agcggctgga aagatgacct tccgctcgaa ggcgagccgc tcgggtccgg
     2281 gaaaggcgac gtcgattccg tagttggccg tcccctcggc aatgagcgac tgcggtgaga
     2341 acaaggggta gacggaatac tcgacccatc cgcgatcccg gacgagcgtt ttctcgagca
     2401 gcgcgttgta gacgtgatgg cccgggtagc cctcgtgaca ggccaggtcg atggccctgt
     2461 cgacatggac cggcaggtcc gtgttcacct ggatgaggct ccggtagccg ccctgatacc
     2521 agttgtacgc gctccagctc ttgtcggtga cgtactcgac ggcgaagctc tcttccgggg
     2581 gaagcgacat gcgctccagg gtccgctgcc ggcatccatc gatggccgcc ttgaacgtgg
     2641 cgtcgagacg ctccttcgga atgacgaacc gcgcgcggaa cgcgtcaagc cgatcgacca
     2701 ggctgccgcg ccccggcagc ctcccctcca ggtccttcag gatggactcg aattcggcct
     2761 cggcgtgcgt cggcgccacg gcgtcgtaca gcgccttcga ctcctcgtcg aagcggagct
     2821 tctcccctcg cagcatcgac acgcgcgcgc gcagcgactg gagctgccgc gtcacgtact
     2881 gatgccggag gcgtacgagc tcttcggcac cggacgccgg acgcgccgcg tcgagcgcgg
     2941 ccaggacgtc cgaggcctcg cggtcgatgt cggcgagctc gcgtctcgcc gtctcggcct
     3001 ccttccgcca cgcaggcggc ccgtaatagg cgtccacgta gtccgcgtcg tgctgcccca
     3061 gggcaagcac gagcttcacg taccgctccg ccaacgcatt catcggatcg ctctccccgg
     3121 atcgatcggc cactccacac gcggacacga gcaccgcccc gacgcacgtc gccaccagtc
     3181 cacgcgccac ggaggcacgg agacaagggc gtggttcgtg atcgcgaatg cgcgtcatct
     3241 atagcgctcc cggactttcg ggatcacata gtgctcgaac gcgccacgaa agtcgtacgc
     3301 gggcgcgaac ccccagtcgc gtcgcgcggc gctgtcgtcc acgtcctcgg gccacgagtc
     3361 aacgacggtc tggcgcttga ggtcggtcgc ccagcgcacc acggccttcg gaaacgccgc
     3421 gacgacggcg tcgtgaatgt cctgcgccgt cgcgctgaag gccgtcaggt tgtacgccgt
     3481 cctcgtgagc gcttcgcgcg gcgcgtcggc caaggcgagg agcgccgaga tggcgtcggg
     3541 catcgccatg aatggaatcc gcgtatcggg tctgacaaag caggtatacg gctcgcccct
     3601 ggctgcggcg tgaatcatct ccggtgcgta gtcggacgtc ccgccggaag gcagcgtgac
     3661 ggcggagatc aggccgggaa agcggaccga gcggaagtcc acgcgcccgg tcgcgtgggc
     3721 ggcgagctgc ttgtagtacc gcgcgtaata tcggcccagg tgctcgcagt aaagcttgtt
     3781 gcagccgtac atcgtgcgcg gcgtgttcca ctcgtcctct cgcacgcgcc cggccgccga
     3841 cttggccgcc aggtccggca gtccgtatgc cgcaaccgac gatgggtaga ggaagaggac
     3901 cgtccggccg tgcgactcgc cctcgcgctg cgcgaactcc agcagcgtca gcgtgccgtc
     3961 gacgttgacg tggtgggcgg taacaggcgt gaactcggac cgcgtggaca acagcgccgc
     4021 gaggtgaaag atcagatcca cctcgaactc ggcgagcacg cgctcgagca ggttcgcgtc
     4081 ggtgatcgac cccgtgaact cgcgcagcac cagcccgccg agcgcaggat ccagcggcac
     4141 gaggtcgacc gtgacaatcg ggcggccggt cccggccagg cgcgtaatca ggccgtggcc
     4201 gatttcaccg cttgctcccg tgaccagcgt gaccggagtt cgcatcggga gatcattcta
     4261 gtctccggga taccgccccc tgccccctgc cccctgcccc ctgccccctg ctaggttcga
     4321 tccccatcga gcagatcgcc gaggccgccc agaatcgagc ccccctcctt gcggctgccg
     4381 ccggctgcgg gagcggcctt gtagatccga tcggccatgc ggctgaaggg cagcgactgc
     4441 agccagacgc gcccgggtcc ggtcagcacc gcgtagaaca gcccttcgcc gccgaacagc
     4501 gccgtcttga tcttgccgac gtactggatg tcgtaggcca ccgacggctg cagggcgacg
     4561 aggcatccgg tgtccacgcg catggtctga cccgcgccga gatccagcgg gtgaaccgtg
     4621 ccccctgcgt gcacgaaaca caggccgtcg ccttcgagct tctgcatgat gaagccttcg
     4681 ccgccgaaca ggccgacgcc cagccgcttc tgaaatgcga tgccgatgct gacgcctttc
     4741 gcggccgcga gaaacgagtc cttctgcgtg atgagggtgc cgccgatctc ggaaaggtgc
     4801 acgggcacaa tcttgcccgg gtacggcgcg ccgaaggcga ccttgcggcg gcgcgtggtg
     4861 cgattgaaga acaccgtcat gaacagcgac tcgcccgtga gcaggcgctt gcccgcgccc
     4921 atcagcgcgt ccatgaaccc gctcttctgc tgccgggagc cgtcgccgaa cacggtctcc
     4981 atctcgatgc cgtcgtccat gaacatcatg ccgccggctt cggcgacggt ggcctcgttg
     5041 ggatcgagct ccacctccac gtactgcatg tcgtcgccgt aaatcttgta gtcaatctcg
     5101 tgcatgtctg ccatctgtgc ggtgcctccg aaaggtcttc atgctatcgc gcgtgcgtgc
     5161 tgggagcctc gcgcttggcg cagtcgccta tgccgctctg gtgactatcg agcttgccca
     5221 cgcccgtcgg agcttcacac gcggtgcccc ccgcatgtat ttcgacacgc cccggactcg
     5281 ccgggagcgg gccggctggt tgattgtctc cgtattggct ggggtcactt gaacgatctt
     5341 cggcgacata aacggcgcga acatctcatc gactcgccgc gcggcgagtt gggattggca
     5401 ggccagcctt tcgcgcctcg tagtgattgt gagcgcggca ccgaagctcg aggttggtac
     5461 tgtcggtccg gccacccgca ctgaacggca cgacgtggtg gaattcgaga aaggccgtct
     5521 cgccgcagcg tccggccggt ccgacaaagg cacactgtcc tgagtcgcgg gcccatacgg
     5581 cgcgtctcac cgcagcgggt acgtatcggc gctccggtgc cgccgccgcc gcggccagcc
     5641 ggggcttgct ggccgctgcg atcctccttc gttccgtgtc gcggaggagt agagccagcg
     5701 cttgatccag aatcgcctcg agatctccgt ccgggtgggt gtgacgtagt agcccgcgaa
     5761 cccgctgcag attgtcgtac gtctcctgac cgacactgac tttcagcaga taccgtctcg
     5821 gcgcaatcgg cacaacgatc ggccgaggtg cagggcgctg ttacggcaag acaggtgcag
     5881 tggcaaccga ctctgacatg gccagcccac tgtcgaggag aacgggcttc ggctccggca
     5941 acgctcggac gctcgcggga atgtctggct gcgggtgcgc cacagcgatc aacccttcaa
     6001 cctcacgagt gctcctgccg cgggcggcct caagcacagc gtccacattc tcgtcggtca
     6061 g
//
LOCUS       DBNGSH010001046         2351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1179716, whole genome shotgun sequence.
ACCESSION   DBNGSH010001046 DBNGSH010000000
VERSION     DBNGSH010001046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1179716"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="AC16CY_23715"
     CDS             <1..878
                     /locus_tag="AC16CY_23715"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR3356881.1"
                     /translation="TLLAAVPAAMAQGQPLMLLGMALVLAGLGFKIAAFPFHMWAPDA
                     YEAGSTPFIAWLSVAPKAAGFVVIIRLYLEGAGNAALAWVPLLSMLAAVTIVAGNLMA
                     IPQRNIKRLLAYSGVAHIGYMLIGLAAMSATGVARVLFYLVAYVFGNMGAFLVVEAVA
                     RSEQSDDIDAYRGLAQRSPLLALAMRLFLLSLGGIPFVAGFWAKLYVFLAGVERGLLG
                     LVFLGAVLTVVALYYYLLVARRMYIEAPERHSQVPMPGLLGTAIAVCVLGVVGIGVYP
                     GPWVDAARRIAATLF"
     gene            1014..1964
                     /locus_tag="AC16CY_23720"
     CDS             1014..1964
                     /locus_tag="AC16CY_23720"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3356882.1"
                     /translation="MRSIQPGPAAIRCALRIAPCVCALVMLGTLAGDARQLRSITDGV
                     YSAAQAERGQRIYLAQCAECHGKAMEGTVGPPMAGDGFLAGWSARPLSGLVDRIQKTM
                     PFNQPGSLSRQESTDLTAYVLQVGRFAAGTSELSEAALSQIAFPASRVPPAVAAATPA
                     GVSLPPPEGNLAELMRAIAFPNANIIFNLQIKNPATQPKKELGASPFDYVEWGATVYP
                     GWLAVDQAAVALVETAPLLLTPGRRCQNGKLAPVERADWKQYVAALADAGRLAYRTSR
                     ARDFEAFGGVSEKLNEACDNCHKVYRDKGGPEGSGAARCQ"
     gene            1979..>2351
                     /locus_tag="AC16CY_23725"
     CDS             1979..>2351
                     /locus_tag="AC16CY_23725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356883.1"
                     /translation="MRRMAFVTAFVILWTSTPSFGQEWIQYASKTDLFAVNFPGEPKV
                     QDVTYTTEFGIGLPGRIYSAGSGGSRYAVTVVDYATAEKLHTERAALCKKNGGEADAC
                     QNDWRGDVQGSIVHASWKLIQK"
BASE COUNT          350 a          788 c          813 g          400 t
ORIGIN      
        1 cgacgctcct ggcggcggtg ccggcggcga tggcgcaggg acagccgctg atgctgctgg
       61 gcatggcgct cgtgctcgcc ggcctcgggt tcaagatcgc ggcgttcccc ttccacatgt
      121 gggcgcccga cgcctacgaa gccggcagca caccgttcat cgcctggctc tcggtcgccc
      181 ccaaggccgc cgggttcgtc gtcatcatcc gcctctacct cgagggcgcg ggcaacgcgg
      241 cgctcgcgtg ggtgccgctc ctgtcgatgc ttgcggccgt gacgatcgtg gcgggaaacc
      301 tgatggccat cccgcagcgg aacatcaagc ggctgctcgc ctactccggc gtcgcgcaca
      361 tcggctacat gctcatcggg ctcgcggcca tgtcggcgac cggcgtggcc agggtgctct
      421 tctatctggt tgcgtacgtc ttcggaaaca tgggggcgtt tctcgtcgtg gaagcggtgg
      481 cgcgctcgga gcagtccgac gacatcgacg cctaccgcgg cctcgcccag cgctcgccgc
      541 tgctcgcgct cgccatgcgg ctgttcctgc tgtcgctcgg aggcattccg ttcgtggcgg
      601 gtttctgggc caagctgtac gtcttcctcg cgggcgtcga gcgaggtctc ctgggcctcg
      661 tgttcctcgg cgccgtgctc acggtcgtgg cgctctacta ctacctcctc gtcgctcgac
      721 gcatgtatat cgaagcgccc gagcgtcact ctcaggttcc gatgccgggc ctgctcggca
      781 cggccatcgc cgtctgcgtg ctcggtgtgg tcggcatcgg cgtctatccg gggccctggg
      841 tggacgctgc ccgccgaatc gccgcgactc tgttctagac gtagtgtccg gcttcagccg
      901 gacccgtgac aggcgtggtg tccggctttc ctggcttgag cggagtcgaa ggcagccgga
      961 cctcgcggca cacgctataa tccgggcgcc tcccgcgagt tggagggttt cacgtgcgct
     1021 ccattcaacc gggcccggcg gcgattcggt gcgcccttcg catcgcgccg tgcgtctgcg
     1081 cgctggtcat gctgggcacc ctggctggag acgcccggca gctgcgatcg atcacggacg
     1141 gcgtctactc cgcggcgcag gcggaacgcg ggcagcggat ctacctggcg cagtgcgccg
     1201 agtgtcacgg caaagcgatg gaaggcactg tcggtccgcc gatggccggc gacggcttcc
     1261 tcgccggctg gagcgcccgc ccgctgtcag gtctcgtgga ccggatccag aagacaatgc
     1321 cgttcaacca gccgggaagc ctgtcccgcc aggaatcaac cgacctcacg gcctacgtcc
     1381 tccaggtcgg ccgattcgcc gccggcacgt cagagttgag cgaagccgcg ctgtcgcaga
     1441 ttgccttccc cgcttcacga gtgcctccgg ctgttgcggc cgcgacgccc gcgggcgtgt
     1501 cgcttccgcc gccggaaggc aacctcgcgg agttgatgag ggccatcgcc tttcccaacg
     1561 cgaacatcat cttcaacctg cagatcaaga accccgcgac gcagcccaag aaggagctcg
     1621 gcgcgtctcc gttcgattac gtcgagtggg gcgccacggt gtatcccggg tggctcgcgg
     1681 tggatcaggc cgcggtggcg ctggttgaaa ccgcgccgct gctgctgacg cccgggcggc
     1741 gctgccagaa cggcaagctc gcgccggtgg agcgggctga ctggaagcag tacgtggcgg
     1801 cgctcgctga cgcgggaagg ctcgcgtacc ggacgtcgcg ggcgagagac ttcgaggcgt
     1861 tcggcggcgt gagcgagaag ttgaacgagg catgcgacaa ctgccacaag gtgtaccgcg
     1921 acaagggcgg ccccgagggg agtggcgcag ccagatgcca atgacgggga ggagtgggat
     1981 gcgccggatg gcattcgtga cggcgttcgt gatcctgtgg acgtccacgc cgtccttcgg
     2041 acaggagtgg atccagtacg ccagcaagac ggatctcttc gcggtcaact tccccggcga
     2101 gccgaaggtg caggacgtca cctacaccac cgaattcggc atcggcttgc cgggccggat
     2161 ctacagcgcc ggcagcggcg gcagccgcta cgcggtcacc gtggtggact acgccactgc
     2221 ggaaaagctg cacaccgaac gggcggcgct ctgcaagaag aacggaggcg aggccgacgc
     2281 ctgccagaac gactggcgcg gcgacgtgca gggatcgatc gtccacgcct cgtggaagct
     2341 cattcagaag a
//
LOCUS       DBNGSH010001047         2216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_840915, whole genome shotgun sequence.
ACCESSION   DBNGSH010001047 DBNGSH010000000
VERSION     DBNGSH010001047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_840915"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="AC16CY_23730"
     CDS             <1..419
                     /locus_tag="AC16CY_23730"
                     /inference="COORDINATES: protein motif:HMM:NF024804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3356884.1"
                     /translation="LAHWCRFCYELRLHRARGYGALKAILDILAEDEGLTLTEISHRL
                     RRTPGSTKDYLSWLEDVDLVVSRQKRYSFTDPVLRLWVRLHCHPVEPTEDDVAREVQR
                     YAIARIPPREAVPALAYAGVDATAEERKSWGIIEID"
     gene            complement(416..1351)
                     /gene="rnc"
                     /locus_tag="AC16CY_23735"
     CDS             complement(416..1351)
                     /gene="rnc"
                     /locus_tag="AC16CY_23735"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR3356885.1"
                     /translation="MPPIVVFSTSSDIEASVVMALLDSQGIESVRVSGNRQGIGPMAV
                     SALGEVRVAVAEEAADEARRIIDSHRQDVGARVVRLRDEFDELQVRIAYRFRDRGLLE
                     HALTHKSRAAEDASGGVADNESLEFLGDAVLGLVVADALFHQYPQYTEGQKSKIKASV
                     VSTHSLARHAERLRLGDHLILGRGEEKTGGRFKQALLADAYEALIAGIYLDGGLEAAE
                     AFLRRELKDAIDEGSAPSFSRDHKSALQERLQALGRPLPDYRVSGETGPDHRKTFTVE
                     VVVNGDVLGAATGRAKKEAEQEAARLALEQLKDSS"
     gene            complement(1435..>2216)
                     /locus_tag="AC16CY_23740"
     CDS             complement(1435..>2216)
                     /locus_tag="AC16CY_23740"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3356886.1"
                     /translation="EKAEKERLEQELLIARSIQMSLLPQGPMRMPGLTLAGHCEPARE
                     VGGDYYDFLPLDDNRMGILIADVAGKGTSAALYMAELKGIVLSLSQQHASPRQLLIDA
                     NRIISKHLGSTSFITMTYAVVDLAARTLTFARAGHCPLVYVPGPHNGSRLGQTLTPEG
                     MVVGLQIDTGDSFPRLLEEMTLPLGRGDLFVLYTDGITEAMNTQGDYFGDARLVELAE
                     QHADLACDELRERILREVHAFAGDAAQHDDMTIVLLKIDDL"
BASE COUNT          342 a          754 c          719 g          401 t
ORIGIN      
        1 cgctcgccca ctggtgccgg ttctgctacg agcttcggct gcaccgcgcg cgcggctacg
       61 gggcgctcaa ggccatcctc gacatcctcg ccgaagacga ggggctgacg ctcaccgaga
      121 tctcgcaccg cctgcggcgt acgccgggct cgacgaagga ttacctgtcg tggctcgaag
      181 acgtcgatct ggtcgtgtcg cgccagaagc gatacagctt caccgacccg gtgctccggc
      241 tgtgggtccg gctccactgc catccggtcg agccgaccga ggacgacgtg gcacgcgagg
      301 tccagcgcta tgccatcgcc cgcattccgc cgcgcgaggc ggtaccggca ctggcctacg
      361 cgggcgtgga cgcaacggcg gaggaacgaa aaagctgggg gattatcgag atcgactaac
      421 tcgagtcctt taattgctcc agcgccagtc gcgcggcttc ctgctcggct tccttcttcg
      481 cccggcccgt cgcggcgccc agcacgtcgc cgttcacgac cacctccacg gtgaacgtct
      541 tccggtgatc gggaccggtt tcgcccgaca cgcggtagtc gggcagcggc cggccgagcg
      601 cctgcaggcg ctcctggagc gccgacttgt ggtcccgcga gaagctcggc gcgctaccct
      661 cgtcgatggc gtccttgagc tcgcggcgga ggaacgcctc ggctgcctcg agtcccccgt
      721 caagatagat cccggcaatc agcgcctcgt acgcgtcggc gagcagcgcc tgcttgaagc
      781 gtccccccgt cttctcttcc ccgcgtccga ggatcaggtg atcgcccagc cgcaggcgct
      841 cggcgtgcct ggccagcgag tgggtggaca cgacgctcgc cttgatcttc gacttctgcc
      901 cctccgtgta ctgcgggtac tggtgaaaga gggcatcggc cacgacgagg ccgagcacgg
      961 cgtctccgag aaactcgagc gactcgttgt cggcgacgcc gcctgaggca tcctcggccg
     1021 cgcgcgactt gtgcgtcagc gcgtgctcga gcaggccgcg atcccgaaac cggtacgcga
     1081 tgcgcacctg gagctcgtcg aactcgtccc gcagccgcac cacgcgcgcc ccgacatcct
     1141 ggcgatggct gtcgatgatg cgccgcgcct cgtccgccgc ttcttcagcg acggccacgc
     1201 gaacctcgcc gagggcgctc accgccatcg gtccgatccc ttgccggttc ccggagactc
     1261 gaacgctctc gatgccctgg ctgtcgagca gcgccatgac gacgctcgct tcgatgtccg
     1321 atgatgtgct gaacacgacg atcgggggca ttgttgatgt tgcgatctgt gattgtcgat
     1381 tcgttgtcga ttggtgattg gtgattgttg atctattgat gattcaatcg ccaatcacag
     1441 atcgtcaatc ttgaggagca ctatcgtcat gtcgtcgtgc tgcgcggcgt cgccggcgaa
     1501 ggcgtgcacc tcgcgcagga tgcgctcgcg gagctcgtcg cacgcgaggt cggcgtgctg
     1561 ctcggccaac tccaccagcc gcgcgtcgcc gaagtagtcc ccctgtgtgt tcatggcctc
     1621 tgtgatgccg tcggtgtaga ggacgaacag atctccgcgt ccaagcggca gcgtcatctc
     1681 ctcgagcaag cgggggaagg agtcgcccgt gtcgatctgc agccccacga ccatcccttc
     1741 cggcgtcagc gtctgcccca gcctcgaccc gttgtgcggg ccggggacat agacgagggg
     1801 acagtggccg gcgcgcgcga aggtcagcgt ccgagcggca agatccacga cggcgtaggt
     1861 catggtgatg aagctcgtcg agcccaggtg cttcgagatg atgcggttgg cgtcgatgag
     1921 cagctgcctc ggggacgcat gctgctggct gagcgagagg acgatgccct tgagctccgc
     1981 catgtacaga gccgcggacg tgcccttccc cgcaacgtcg gcgatcagga ttcccatccg
     2041 attgtcgtcg agcgggagaa agtcgtagta gtcgcctccc acttcccgtg cgggctcgca
     2101 atgtccggcc agcgtcaggc ccggcatgcg catcggcccc tgcgggagga gcgacatctg
     2161 gatgctgcgc gcgatcagga gctcctgctc cagccgctcc ttctcggcct tctcac
//
LOCUS       DBNGSH010001048         3750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_331644, whole genome shotgun sequence.
ACCESSION   DBNGSH010001048 DBNGSH010000000
VERSION     DBNGSH010001048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_331644"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="AC16CY_23745"
     CDS             <1..1226
                     /locus_tag="AC16CY_23745"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3356887.1"
                     /translation="LGLRWAGVDRETLQKLARELRAAQQPDGGWNSVKGRPSDAYSTA
                     QALVALRDGGLVAIIDASYQRGAAFLLKTQAADGTWHVPSRLHAPAPVSPPFFESGYP
                     HGHDQFLSMQGAAWAVMALSYLLEPGVPAAPPPLPETAPSGVEPWAETMIFGTVDEVR
                     KLLDGGLSPNAATKTGGTTALMMAAPDPAKMQLLLDRGADVNARAASRYSALMVAAQY
                     QDGEAAINLLLDRGARATPPESGAPVFNANPFFLAAYAGNAKSLRRLLAAGGKIDEAM
                     IAIGTSRTTPMLGAFKFGDAEVARALFDLGAPIDFADGNGITMLGRSVLNNEIEMARM
                     LIARGANVNVVDKQGMTPLLWAANIDFGDASMVELLLGAGARADARNKDGLTPLELAR
                     KYGHTNLIPALERGK"
     gene            1316..1846
                     /locus_tag="AC16CY_23750"
     CDS             1316..1846
                     /locus_tag="AC16CY_23750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3356888.1"
                     /translation="MPDEVESDLLERFVHGDQAAFETLFRRFQSEVHRWIARIVRDAG
                     TADDVLVEAFWRAYRARGRFDPSRSFGAWMRRIATNAALDAVRHARAARWRAIDDTLP
                     AVGGPDPDVRDAIVAAFRRLPPKLQVAATLALVEEVPYAEIADALDIPIGTVKSRVSR
                     ATGLLRTELARRGVRP"
     gene            1843..2055
                     /locus_tag="AC16CY_23755"
     CDS             1843..2055
                     /locus_tag="AC16CY_23755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356889.1"
                     /translation="MTDDDRLVTLLRSAMPPVPGDGPVRDLWPAVVDRARPPRAWSWL
                     DLAVAAAVVAAGVMFPGWLVSLAYYL"
     gene            2067..2501
                     /locus_tag="AC16CY_23760"
     CDS             2067..2501
                     /locus_tag="AC16CY_23760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356890.1"
                     /translation="MRPRPYLRAYMAGIVVPTLFLLVIVTVDAYQRYYFEVPNQFVLG
                     YSTAPLERAILFPMAIVPNVWGAWNMLYLRIRSRVRWSVGVHGSLLVLLLIPGGVALA
                     RAFDVFTIQLQFALPMVPIGMGIYYLAWKHLVGRLNAEMGIA"
     gene            2633..3142
                     /locus_tag="AC16CY_23765"
     CDS             2633..3142
                     /locus_tag="AC16CY_23765"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR3356891.1"
                     /translation="MLIEARLADITTLEVDAIVNAANTSLAPGGGVCGAIHRAAGPEL
                     AEACARAAPCPTGQARLTRGFKLPAKVVIHAVGPVWRGGNAGEAALLAAAYRSSLALA
                     REHQLASIAFPAISTGIFGYPLREATEIAVAAVRAEAAIPGSLDRVVFACFNREVLGA
                     YETALSPHP"
     gene            3188..3652
                     /locus_tag="AC16CY_23770"
     CDS             3188..3652
                     /locus_tag="AC16CY_23770"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3356892.1"
                     /translation="MSEHKATVRWTHSQGDFLKGSYSREHTWTFDGGATVPASPSPSA
                     VKPPYCNPAFVDPEEAYVASISSCHMLTFLWFASRQGVDVASYEDEAVGLLTKNDRGV
                     PWVSSVVLRPRIVYVGTPPSPEVEEQLHHKAHEGCFIANSVKTTITVEGTNH"
BASE COUNT          581 a         1270 c         1266 g          633 t
ORIGIN      
        1 tgctcgggct ccgctgggcc ggcgtcgatc gcgaaacgct gcagaagctg gcgcgcgagc
       61 tgagggcggc gcagcagccc gatggcggct ggaactcggt caaggggagg ccgagcgacg
      121 cctactcgac ggcccaggcg ctcgtggccc tccgcgacgg cggtctcgtg gccatcatcg
      181 atgcgtcgta tcagcgcggc gccgcgttcc tcctgaagac gcaggccgcc gacggcacgt
      241 ggcacgtgcc gtcgcgcctg cacgcgccgg ccccggtcag tccgccgttc ttcgagtcag
      301 gctatccgca cggccacgat cagttcctgt cgatgcaggg cgcggcctgg gccgtgatgg
      361 cgttgtcgta cctgctcgag cctggcgttc ccgccgcgcc gcccccgctg cccgagaccg
      421 cgccgtcggg cgtcgagccg tgggccgaga cgatgatctt cggcactgtg gacgaggtgc
      481 gcaagctgct cgacggcggc ctcagcccga acgcggccac caagaccggc gggaccacgg
      541 ccctgatgat ggcggcgccc gatcccgcga agatgcagct gttgctcgac cgcggcgcgg
      601 acgtgaatgc ccgtgccgcc agccggtatt cggcgctgat ggtggccgcg cagtatcagg
      661 acggcgaggc cgccatcaat ctgctgctcg accgtggggc gcgggccacg ccgcccgagt
      721 ctggcgcgcc ggtcttcaat gccaacccgt tcttcctcgc cgcgtatgcc ggcaacgcga
      781 agagcctcag gcggctgctc gcggcgggcg gcaagatcga cgaggcgatg atcgccatcg
      841 ggacgtcgcg taccaccccc atgctcggcg cgttcaagtt cggagatgcc gaggtggccc
      901 gcgcgctgtt cgacctcggc gcacccatcg actttgccga cggcaacggc atcaccatgc
      961 tgggccgctc ggtcctgaac aacgagatcg agatggccag gatgctcatt gcccggggcg
     1021 ccaacgtcaa cgtcgtagac aagcagggga tgacgccgct gctgtgggcc gccaacatcg
     1081 acttcggcga cgcgtcgatg gtcgagctgc tgcttggcgc cggcgccagg gccgacgcgc
     1141 gcaacaagga cggactcaca ccgctggagc tggcgcgcaa gtacggccac acaaatctga
     1201 tcccagccct cgagcgcggg aagtagaccc tccgatcctc gattcgtacg tagtgtccgg
     1261 cttcagccgg accctggaac ctttgccacc cccgccgagt aaggcagata gggccatgcc
     1321 ggacgaggtc gagagcgacc ttctcgagcg cttcgtgcac ggggaccagg ccgctttcga
     1381 aacgctcttc cgccggttcc agtcggaggt ccatcgctgg atcgcgcgaa tcgttcgcga
     1441 tgccggcacg gcggacgacg tgctcgtgga agcgttctgg cgggcgtatc gggcgagggg
     1501 ccggttcgac ccttcccgca gtttcggcgc gtggatgcgc cgaattgcga ccaacgcggc
     1561 gctcgacgcg gtgaggcacg cgcgcgccgc gaggtggcga gcaatagacg acaccctccc
     1621 ggccgtgggc ggtccggacc cggacgttcg tgacgccatc gtggccgcgt tccggcgtct
     1681 gccgccgaag ctgcaggtgg cggcgacgct cgcgctggtg gaagaagtgc cctacgccga
     1741 gatcgccgat gcgctcgaca tcccgattgg cacggtgaag tctcgcgtct cccgggcaac
     1801 cggcctgctg aggacggagc tggcgcgacg tggagtgcga ccatgaccga cgacgaccgg
     1861 ctcgtgacac tgctgcgatc ggcgatgccg ccggtgccgg gcgacggacc ggtacgcgat
     1921 ctgtggccgg ccgtcgtcga tcgcgcccga ccgccgcgtg cgtggtcgtg gctcgacctg
     1981 gcggtcgccg ccgcagtggt cgccgccggc gtgatgtttc ccggctggct cgtctcgctt
     2041 gcttactatc tctgacggag gacgtcatgc gaccccgacc atacctgcgc gcgtacatgg
     2101 cgggcatcgt cgtgcccacg ttgtttctgc tcgtcatcgt gacggtggat gcctatcagc
     2161 gctactactt cgaggtgcca aaccagttcg tgctcgggta ctcgacggcg ccgctcgagc
     2221 gggcgatcct gtttccgatg gccatcgttc ccaacgtctg gggtgcctgg aacatgctct
     2281 accttcgaat ccgatcccgg gtgcgctggt cggtcggcgt gcacggctcg ctgctcgtgc
     2341 tcctgctgat cccaggggga gtggcactgg cccgggcgtt cgacgtgttc accattcagc
     2401 tgcaattcgc cctcccgatg gtgccgatcg gcatggggat ctactacctg gcgtggaagc
     2461 atctggtcgg gcgcttgaat gcggagatgg gaattgcttg acgagccctg gagatgccga
     2521 tgacgtacgc taaccacaga gtggcagagg acacagagtg ttgcttcgat ctgatgattc
     2581 agatcctctg tgttctctgt gcctgtggtt agcgtccgtg tcgccaaatg tcatgctgat
     2641 cgaggcgcgg ctcgccgaca tcacgacgct cgaggttgac gcgatcgtca acgccgccaa
     2701 cacgagcctg gcgcccggcg ggggagtgtg cggcgccatc catcgggcgg ccgggccgga
     2761 actcgccgag gcctgcgcgc gtgcggcgcc gtgccccaca gggcaggccc ggctgacacg
     2821 cggcttcaag ctgccggcga aggtcgtgat ccatgccgtg ggccccgtgt ggcgtggcgg
     2881 gaacgcgggc gaggccgcac tgctcgcggc cgcctatcgc agctcgcttg cgctggcacg
     2941 cgagcaccag ctcgccagca tcgcgtttcc agccatcagc acgggcatct tcggatatcc
     3001 gcttcgcgag gcgaccgaga ttgccgttgc cgcggtgcga gccgaggccg ccatcccggg
     3061 ctcactggat cgtgtggtct tcgcgtgctt caatcgagag gtcctcggcg cgtatgagac
     3121 tgccctgtcc cctcacccct gacccctaac ccctaacccc taacccctac cccctacacc
     3181 aaccaccatg tccgaacaca aggccactgt cagatggacg cattcgcagg gcgatttcct
     3241 caaggggagc tactcgcgcg agcacacctg gacgttcgac ggcggggcga cggtccccgc
     3301 gtcgccgtcg ccctccgccg tcaagcctcc atactgcaat ccggccttcg tcgatccgga
     3361 ggaggcgtac gtcgcgtcga tctcgagctg ccacatgctg acgtttctct ggttcgcctc
     3421 acgtcagggc gttgacgtcg cgagctacga agacgaggct gtcggtctgt tgacgaagaa
     3481 cgaccgaggc gtcccctggg tcagttcggt cgtcctccgc ccgcgcatcg tctacgtggg
     3541 cacgccgccg tcgcccgagg tcgaggagca gcttcaccac aaggcgcacg aggggtgctt
     3601 catcgccaat tccgtgaaga cgaccattac cgtggaaggc acgaaccact gaggcacgga
     3661 gacaaggaga gattgacgtc agccttctgc gccgtcaatg ctccggtggg aagaggtcgt
     3721 ccacgggcaa agaccagccg ggcaaggccg
//
LOCUS       DBNGSH010001049         2183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1012173, whole genome shotgun sequence.
ACCESSION   DBNGSH010001049 DBNGSH010000000
VERSION     DBNGSH010001049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1012173"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1054)
                     /locus_tag="AC16CY_23775"
     CDS             complement(95..1054)
                     /locus_tag="AC16CY_23775"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3356893.1"
                     /translation="MTPLRAVEGGHVTLRGSGFPVDRVPRVSVGGQPARVAFASSSRL
                     VVIVPPDVETGRVPIRVEDLPGETAFVTVGTAWATGLHQVDNPVFDADGNLFVTYSGS
                     RGQEAPVSIFRVTREGTREPFVTGIVNATSMTIGPDGRLYVSSRFEGSVHRVKPDGSH
                     ELVVSDLGVACGLAFDRDGALYVGDRSGTIFRVREGRAEQFATLPPSVAAFHLAIGPD
                     GDLFVSVPTLATYDHVYRIGPNGQVRSVLASFGRPQGLAFSPDEGVLHVTDALAGRSG
                     LYRLKDLDGEPELIASGCGFIGVAFGPGGQMVVASNDTAYRFD"
     gene            complement(1085..1618)
                     /locus_tag="AC16CY_23780"
     CDS             complement(1085..1618)
                     /locus_tag="AC16CY_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3356894.1"
                     /translation="MQQALLLNASYEPLKVVHWQKAITLWCQGKVEVIAVHDREVRAV
                     SVSFKLPSVIRLLRFVKIKRRFDYVPFSRANIYARDRNTCQYCNESLPTSDLTFDHVV
                     PVAQGGRKDWENIVTCCISCNRLKGGRTPAEAGMKLLRAPRRPDSAPAIRITIGLRDA
                     PESWRDYLYWNVELEDT"
     gene            1714..>2183
                     /locus_tag="AC16CY_23785"
     CDS             1714..>2183
                     /locus_tag="AC16CY_23785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3356895.1"
                     /translation="MTARDLVRLLVSSVLAAGVTACASGGNAPRPRPFPGANAPPAVK
                     TAEVEPEDTQPPLLATALALRGTPYLNGGSEPTQGFDCSGFVQWVFGQHGTPLPREVR
                     KQFDEGRGIDRDEVKAGDLVFFTTVSRGASHVGIALGNGEFVHAPSSRGVVRVEP"
BASE COUNT          381 a          734 c          727 g          341 t
ORIGIN      
        1 ttggtggttg gtggatagtg gaagtggttg gtggtcggcg gactcgcggt gaatattagc
       61 agccactcac cactaaccac taaccactca ccgctcaatc aaaccgatag gcggtgtcgt
      121 tggacgccac gaccatctgg ccgcccggcc cgaatgcgac gccgatgaac ccgcagcccg
      181 acgcgatcag ctcgggctcg ccgtccaggt ccttgaggcg gtacagaccg cttctgccgg
      241 ccaaggcgtc tgtcacgtga agcacgccct cgtcgggcga gaacgcgagc ccctgcgggc
      301 ggccgaacga cgcgagcacc gagcgtacct ggccgttggg tccgatgcgg tacacgtggt
      361 cgtaggtggc aagcgtcggc acgctcacga acaggtcgcc gtcaggacca atcgccagat
      421 ggaaggccgc cacgctcggc ggcagcgtgg cgaactgctc ggcgcgaccc tcgcgaacgc
      481 ggaagatcgt tccggatcgg tctcccacat acagcgcgcc gtcgcggtcg aaggccaggc
      541 cgcacgccac gccgaggtcc gacaccacga gctcgtggct gccgtccggc ttgacgcgat
      601 ggaccgaacc ttcgaaccgg ctcgacacgt acaaccggcc gtccggcccg atcgtcatcg
      661 aggtggcatt cacgatgccc gttacgaacg gctcccgcgt gccctcgcgc gtgacgcgga
      721 agatcgacac tggagcctct tgcccgcgcg atccgctgta ggtcacgaac agattgccgt
      781 cggcgtcgaa gacgggattg tccacctggt gaagacccgt cgcccaggcg gtccccaccg
      841 tgacgaacgc cgtctcgccg ggaagatcct cgacgcgtat cggcacgcgc ccggtctcga
      901 catcgggagg cacgatcacg acgaggcggg agctcgaggc aaatgcgacg cgggccggct
      961 gcccgcccac gctgacacga ggaacccgat cgacgggaaa ccccgatccc ctcagggtca
     1021 cgtggccgcc ttcgacagcg cggagcgggg tcaccgaggc gatggtggag cggggcaaag
     1081 cgccctacgt gtcttccagc tcgacgttcc aatacagata gtcgcgccag ctctccgggg
     1141 cgtcgcgcag cccgatcgtg atcctgatcg cgggcgcgga atcgggccgg cgcggcgccc
     1201 gcagcagctt catgcctgcc tccgcgggcg tgcggccgcc cttgagccgg ttgcacgaga
     1261 tgcagcacgt cacgatgttc tcccagtcct ttcgtccccc ttgcgccacg ggcacgacgt
     1321 ggtcgaaggt gagatccgag gtggggagcg actcgttgca gtactggcag gtattgcggt
     1381 cgcgcgcgta gatgttggct cgcgagaacg gcacgtagtc gaatcgtcgt tttatcttga
     1441 cgaagcgcag caggcggatc accgacggca gcttgaagct caccgacacc gcgcgtacct
     1501 cgcggtcgtg gaccgcgatc acctccactt tcccctggca ccagagcgtg atcgctttct
     1561 gccagtggac gaccttgaga ggctcgtacg acgcgttcag aaggagcgcc tgttgcatga
     1621 cctgaggcca tcatgccgga cgtcggctgg actgtcaagc gagcggacgc gttaaactct
     1681 aagcaacatg agggttacgc cgataggatg tccgtgactg cacgcgatct cgtccgcctg
     1741 ctcgtgtcat ctgtccttgc agcaggagtc accgcctgtg caagcggggg gaacgcgccg
     1801 cgaccgcggc cgttccctgg agcgaacgcc ccgccggccg tcaagacggc ggaggtcgag
     1861 ccggaggaca cccagccgcc tctcctggcc acggcgctgg cgctgcgcgg gacgccgtac
     1921 ctcaacggcg gaagcgaacc cactcaagga ttcgactgca gcggcttcgt gcaatgggtg
     1981 ttcggccagc acggaacgcc gctcccccgt gaagtgcgca agcagttcga tgaggggcgc
     2041 gggatcgacc gcgacgaggt caaggccggc gacctcgtgt tcttcacgac ggtcagccgc
     2101 ggcgcgtcgc acgtcggcat cgcgctcggc aacggcgagt tcgtccacgc gccatcctcg
     2161 cgaggcgtcg tgcgggtcga gcc
//
LOCUS       DBNGSH010001050         2461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1350384, whole genome shotgun sequence.
ACCESSION   DBNGSH010001050 DBNGSH010000000
VERSION     DBNGSH010001050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1350384"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="AC16CY_23790"
     CDS             complement(<1..763)
                     /locus_tag="AC16CY_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356896.1"
                     /translation="MGVCALIAAVLSVGAWAQEMPDPSQIHGRALPAPELPNGTVTVR
                     VVREAIGNNVPGQQVRVTAGGATRLAATDDQGRAEFKDLAAGGTSAVAEVTVDGERLT
                     SQPFTMPDSGGLRVILVAGLARAAERRKLEEAAAAAEPPVKGIVVFGGNSRVLMQFND
                     DALDVYYVLEIINSARARVDIGGPLVIELPTAAAGASVLEGSSPSASVEGNLVTIAGP
                     FASGTTAVQVAYRIRSSDSDLTISQTWPAALPQVIV"
     gene            complement(847..1491)
                     /locus_tag="AC16CY_23795"
     CDS             complement(847..1491)
                     /locus_tag="AC16CY_23795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3356897.1"
                     /translation="MSSETSTEPQVRLKPDTTYEGTLAPRSPESRAQVSAAFEPWQLF
                     TLAGLIGATIAVFMSRGQSPAAVILLSLIVFAASAVGIAALRMVLPLVQKGTAVEGPQ
                     VLGGRTRAGLEREKTLALRSIKELEFDRAMGKVSEKDFAEMSGRLRSRAARLMRQLDA
                     GAGYRDEIDREIVKRIGAAPTPPSPHGACPSCATVNDADARFCKQCGRALEARS"
     gene            complement(1469..>2461)
                     /locus_tag="AC16CY_23800"
     CDS             complement(1469..>2461)
                     /locus_tag="AC16CY_23800"
                     /inference="COORDINATES: protein motif:HMM:NF015853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR3356898.1"
                     /translation="LVGLVGRNKRRYGGYLVHVGIVLMFLGFAGSGYKQKQIVEATPG
                     QNVTIGDYTVRFDQLKVTQDERKQMVTSEMTVFRNGAELARMYPARWYFHGRESEPTS
                     EVAIRRSFGEDLYLVMSDFNLAEQRASLEVHVNPLVNWLWLGFGMLAFGTFIALLPDR
                     AFAFAEAKVPAHAATASMLLLAAIVWPAAILAQEAAPRVRFTALEEQVRGEVMCTCSC
                     RRSLTFCGMANCHGEAEQMTRLRAYIAEGKSHDEILASFVNDMGAEILMTPPDTGFNR
                     LAWLLPYLFGAAGLVAIVMTARRWSRPAAAVAGADATLDPALDARLDDELRDLD"
BASE COUNT          398 a          861 c          812 g          390 t
ORIGIN      
        1 cgacgatgac ctgcgggagc gctgccggcc aggtctgcga gatcgtgagg tcgctgtccg
       61 agctgcgtat tcggtacgcg acctgcacgg cggtcgtgcc ggacgcgaac ggaccggcaa
      121 tcgtcacgag attaccctcg actgacgctg acggcgacga cccttcgagc acgctcgcgc
      181 cggccgccgc agtcggcagc tcgatcacca gcgggccgcc gatgtccacg cgcgcccgag
      241 cgctgttgat gatctcgagc acgtagtaca cgtcgagcgc atcgtcgttg aactgcatca
      301 gcacgcgcga gttgccgccg aacacgacga tgcccttgac gggcggctcc gcggctgctg
      361 ccgcctcctc gagtttgcgc cgctccgccg cccgagccag gccggcgacc aggatcactc
      421 ggagtccgcc cgagtctggc atcgtgaacg gctgcgaggt gaggcgctcg ccatccacgg
      481 tgacttcggc caccgcgctc gtacctcccg ccgccagatc cttgaactcc gcgcggcctt
      541 gatcgtcggt cgcggcgagc cgcgtcgcgc caccggccgt gacccgcacc tgctggccgg
      601 gtacgttgtt gccgatcgcc tctcgaacca cccgcaccgt caccgttccg ttcggcagct
      661 ccggtgccgg cagggctcgg ccgtggatct gcgatgggtc gggcatctcc tgcgcccacg
      721 cgccaacgga caggacggcg gcgataaggg cgcaaactcc caacgcccaa atcccaattc
      781 ccaaccgact gcgttgagag gacagattct gcatttggga gttgggaatt gggggttggg
      841 agttgctcat gaccgcgcct ccagagcccg cccacactgc ttgcagaacc gtgcgtcggc
      901 gtcgttgacg gtcgcgcacg aggggcacgc gccgtgaggc gacggcggcg ttggggcggc
      961 cccaattctc ttgacgattt cgcggtcgat ttcgtcgcga tagccggcgc ccgcgtcgag
     1021 ctgacgcatg agccgcgcag cacgggagcg cagccgtccg ctcatctcgg cgaagtcctt
     1081 ctcggagacc ttccccatcg cccgatcgaa ctcgagctcc ttgatcgatc tgagcgcaag
     1141 cgtcttctcg cgctccaggc ccgcgcgcgt acgccccccg agcacctgcg ggccttcgac
     1201 ggcggtcccc ttctgcacga gcggcagcac catgcggagc gcggcgatgc caaccgcgga
     1261 ggcggcgaag acgatgaggc tgagcaggat gacggcggcg ggcgactggc cgcgcgacat
     1321 gaagacggcg attgtcgccc cgatcaggcc ggccagcgtg aagagctgcc acggctcgaa
     1381 tgcggcgctg acctgcgccc tcgattccgg gcttcgaggc gccagcgtac cttcgtacgt
     1441 agtgtccggc ttcagccgga cctggggttc agtcgaggtc tcggagctca tcgtccaggc
     1501 gggcgtcgag cgccggatcg agcgtcgcgt cggcgccagc caccgcggcg gcggggcgcg
     1561 accagcggcg ggcggtcatg acaatcgcca cgagtccggc cgccccaaac aggtacggaa
     1621 gaagccaggc cagcctgttg aacccggtgt cgggcggcgt catcaggatc tctgcgccca
     1681 tgtcgttgac gaacgacgcc agaatctcgt cgtgcgactt cccctccgcg atgtacgcgc
     1741 ggagccgtgt catctgctcg gcctcgccgt ggcaattggc catgccgcag aaggtcagcg
     1801 accggcggca cgagcacgtg cacatgacct cgccccggac ttgctcttcg agcgcggtga
     1861 acctgacgcg gggcgcggcc tcctgcgcca gaatcgccgc cggccagacg atcgccgcca
     1921 gaaggagcat cgatgccgtg gccgcatggg ccggcacctt ggcctctgcg aacgcaaacg
     1981 cgcggtccgg aagcagcgcg atgaacgtgc cgaacgccag catgccaaag cccaaccaga
     2041 gccagttcac gagcgggttg acgtggacct ccaggctcgc gcgctgctcc gccagattga
     2101 agtccgacat cacgagatac aggtcctcgc cgaacgagcg gcggatggcc acctcggacg
     2161 tcggttcgct ttcgcggccg tggaagtacc atcgcgcggg atacatccgg gccagctcgg
     2221 cgccgttgcg gaacacggtc atctcgctcg tgaccatctg tttccgctcg tcctgcgtca
     2281 ccttcaactg gtcgaaccgg accgtgtaat cgccgatcgt gacgttctgg cccggcgtgg
     2341 cctcgacgat ctgtttctgc ttgtagcccg agccggcaaa tcccaggaac atcagcacga
     2401 tgccgacgtg aacgaggtag ccgccgtagc ggcgcttgtt gcgcccgacc aggccgacca
     2461 g
//
LOCUS       DBNGSH010001051         6251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3388965, whole genome shotgun sequence.
ACCESSION   DBNGSH010001051 DBNGSH010000000
VERSION     DBNGSH010001051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3388965"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC16CY_23805"
     CDS             complement(<1..202)
                     /locus_tag="AC16CY_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3356899.1"
                     /translation="MGKTTTAINLATSLALAGRRVLLVDVDPQGNLTSGIGFKSQRSD
                     GGTVYEALLAGGPPESFVLPTRI"
     gene            complement(404..1033)
                     /locus_tag="AC16CY_23810"
     CDS             complement(404..1033)
                     /locus_tag="AC16CY_23810"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR3356900.1"
                     /translation="MAISAEVGTQLEVYYRLLVAWNHRINLTGLDLESLGPEALDRLL
                     VEPLVAAKHVSAEAGRAIDIGSGGGSPAIPLMLALPGIRLVMVESKTRKSVFLREVVR
                     ALGHARSEVVTARFEELLADPRLHEAHDVLTIRAVRVESRVLMGLQAFVRPRGELLLF
                     RSAGGLGGPTTLIPPLRWKATYPLIEAHGSQLIVLEKHSTRDQHAGRST"
     gene            complement(1084..2946)
                     /gene="mnmG"
                     /locus_tag="AC16CY_23815"
     CDS             complement(1084..2946)
                     /gene="mnmG"
                     /locus_tag="AC16CY_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR3356901.1"
                     /translation="MPLKFDVVVIGAGHAGVEAAHAAARLGCHVGLCTLSLKTVAHMP
                     CNPAIGGTAKGHLVREIDALGGLMGRAIDATGIQFKLLNRSRGPAVWSPRAQADKRTY
                     GDWIRQAIGQHPNIEWILEPASRLLVESGRIVGLAIEGGQRLQCPAVVVTTGTFLNGL
                     IHEGPEQRPAGRHGEPPSISLAESIRDLGFEVGRLKTGTPPRLDRRSIDFQRQVEAGT
                     FVEERGDPHPTPFSYRTTGQLRNHISCWLLHTNDRVRELVRDNIAQSPLYNGQIRGIG
                     PRYCPSLEDKIMRFPDRERHQIYLEPEGVDAREIYVNGLSMSLPRPVQEALVRALPGL
                     EHAVMLRPGYAVEYDFVQPTELTACLEAHRVRGLYLAGQVNGTSGYEEAAAQGLVAGI
                     NAARQARGESGFVLGREESYIGVLVDDLVTRGCLEPYRMFTSRAEYRLLLRIDNADLR
                     LTPKGRSLGLVDDEQWDVFRARRARYESNAEALETSRVRAPDGRSTTAARLLKQPEVR
                     LADLIADRRVHLKIDANRAELDVTSLETAIKYAGYLEQEEARAERAKREDRRRIPQGF
                     SFQSIPGLSREVVQRLTQCRPSTLGQAARIPGVTPAAVAVLSVFLRRQSPQVSA"
     gene            complement(2946..4295)
                     /gene="mnmE"
                     /locus_tag="AC16CY_23820"
     CDS             complement(2946..4295)
                     /gene="mnmE"
                     /locus_tag="AC16CY_23820"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR3356902.1"
                     /translation="MFSSDDTIAAIATPEGRGGIGIVRLSGPDALPIATRILARREKL
                     EPRHATLTKVRLDRSASGVGDQVIATWFPAPQSYTGEHVVEISAHGSPVVLRAILESL
                     VGEGARLARPGEFTFRAYLNSRLDLIQAEAVADLVDAVTPLQARVAFAQLEGTLSDQV
                     RRLDAELLDLIARLEASLDFPDEGFHFIEPHEVGGRIGAVLACLEVVLQHSEHGRVVR
                     EGAKVAIVGRANVGKSSLFNALLGEPRAIVTGVPGTTRDLVTEQVEIDGLAVTLIDTA
                     GLRSTGDVVEQEGVNRAMGAIEAADLLLVVIDSGDDLDDEDRRLLEDVPSARRLVIAS
                     KSDRRSRWDLEGAIRVSTLTNSGIGTLKQSIVRALSGGDAWRDTPAISNLRHASLLRR
                     AQHSLRRAQAAAGEHGAPEEFVLVDLHQARDHFEEVVGARTNEDVLRHIFDRFCVGK"
     gene            complement(4343..4798)
                     /locus_tag="AC16CY_23825"
     CDS             complement(4343..4798)
                     /locus_tag="AC16CY_23825"
                     /inference="COORDINATES: protein motif:HMM:NF013583.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Jag family protein"
                     /protein_id="HIR3356903.1"
                     /translation="MNQTLAAQIADFVQHVVNAMGVGLTANIEEKPEGTRINLEGEDG
                     GLLIRRGGEGLQALQHIVATAFRRQLGDDNRVVIDCNGFRKDKDAEIRQMARFMAEKA
                     RQSGLPQEMGPMNPYERRIVHLAIAEDPTVSSESIGDAFLKTVIISTKQ"
     gene            complement(4861..>6251)
                     /gene="yidC"
                     /locus_tag="AC16CY_23830"
     CDS             complement(4861..>6251)
                     /gene="yidC"
                     /locus_tag="AC16CY_23830"
                     /inference="COORDINATES: protein motif:HMM:NF026199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR3356904.1"
                     /translation="VPADQPRPFSIVADDQGVTRRLNSALYKVTGDADGRVDAATTSA
                     SLVFEFQDASGLRVRKEFTFEPKNYVVTFTAAVSNNAGPLRTRIAWGPGLGDAGAMAG
                     GGSFFTGNYVQPPQAIYYRDGEVSRVPASTLQEQPVHEGSFRWAGVDDHYFIAAAITP
                     GQARLEFHPLTLPVSDSRQRNLVTQTIQLSNASERLRFFIGPKQFEVLREVDQELVRA
                     INFGMFGFLSVPLLNALKWVHTYVGNWGWSIVVLTILINLAIAPLRHKSVVSMRKMQA
                     LQPQLKAIQDHYAGLKVTDPARQKMNSEIMSLYREKGVNPASGCVPMLLTLPILFAFY
                     SLLSQAIELRGAEFTLWIKDLSEHDPLYVTPVLMAATMFWQQWITPTTADPAQQKIMM
                     MMPLMFGVMFLWMPSGLVVYWFVGNLWAIGQQYFTNWLIGPPVAAGTRPPAERRLKSA
                     GTGRTAAAERKN"
BASE COUNT         1111 a         2003 c         1926 g         1211 t
ORIGIN      
        1 ggatacgcgt gggaagcaca aacgactccg ggggaccgcc ggcgagaagc gcctcgtaga
       61 cagtgcctcc atcggatcgt tgagacttga agccgatgcc gctggtcaga tttccctgtg
      121 gatcaacgtc gaccagcagc actcgtctac ccgccaaggc aagcgaagta gccaggttga
      181 tggccgtcgt tgtcttgccc acgccaccct tctgattggc aatcgctacg attcgggcgc
      241 caaatgcacc agtcgatggc ggcggtgatt ctgtcatatg aactaaatat ttggactgtc
      301 tctacctgcg gtgttgcacc tgatgaatct agggctcatt ggctctcacg tcaagggctg
      361 ttccacgtgg aacatggcga acataaagcg atcctctggt gtttcacgtg gaacgtcccg
      421 catgctggtc tcgggtcgag tgcttttcca acacaatcag ctggcttccg tgagcctcga
      481 taagtggata ggtcgccttc cacctcagtg gcggaatgag tgtcgtcggt ccaccaaggc
      541 ctcccgctga acggaagagg aggagttcgc ctctcggtcg cacgaaggcc tgtagcccca
      601 tcaacactcg cgactcgacg cggacggccc gaattgtcaa cacgtcgtgc gcctcgtgca
      661 gccgggggtc cgccaggagt tcctcgaacc ttgcggtgac gacctcgctt cgagcgtgtc
      721 cgagagcccg aacgacctcc cggagaaaca ccgattttcg cgtcttggac tcgaccatca
      781 cgagacggat tcccggtagg gccagcatga gcggaatcgc cggcgagcct cccccgcttc
      841 cgatgtcgat ggcgcgtcca gcctccgcag agacatgctt ggcggcgacg agtggttcca
      901 cgagcaggcg gtcgagcgct tccgggccga gactctccag gtcaagtccg gtcaggttta
      961 tccgatggtt ccaggcaacg agaagccggt aatagacttc gagctgtgtc cccacttccg
     1021 ccgaaattgc cacgccagcg cgccgggcgc gtcgcgtcag ccgctgttga aactcacggc
     1081 tggtcacgcg ctgacctgtg ggctttgtcg tcgcagaaag acagagagaa cggcaacggc
     1141 ggcgggtgtg acacctggta tgcgggccgc ctgtccgagc gttgacggcc ggcattgtgt
     1201 gagacgttgc accacctcgc gtgacaaccc gggaatggac tgaaacgaga agccctgtgg
     1261 aatgcgtcgg cggtcctccc tcttggcccg ttccgcccgc gcctcctcct gctcgagata
     1321 tccggcgtac ttgatcgccg tctcgagact cgtcacgtcg agctcggcac gattcgcgtc
     1381 gatcttgagg tgaacgcgac ggtcggcaat caggtctgcc agcctgactt caggctgttt
     1441 caggaggcga gctgcagtcg tgctccggcc atcgggggcg cgcactcggc tcgtctcgag
     1501 cgcttcggcg ttgctctcgt agcgggcgcg ccgcgctcga aagacgtccc attgctcgtc
     1561 atcgacgagg ccgagcgatc gccctttggg cgtcaagcgg agatccgcgt tgtcgattcg
     1621 aaggagcagg cgatactccg cccgggacgt gaacatgcga tacggctcca ggcaacccct
     1681 cgtgacgaga tcgtctacga gcacgccgat gtaagactcc tcgcggccga ggacgaatcc
     1741 ggactctcct cttgcctgcc gagcggcatt gatgcccgcg acgaggcctt gcgctgcagc
     1801 ctcctcgtag cctgaggtcc cattcacctg cccggccagg tagagcccac gaacgcggtg
     1861 ggcttcgagg cacgctgtca gctcggtcgg ctgaacgaag tcgtattcga cggcgtagcc
     1921 tggccggagc atgaccgcgt gctcgagccc tggcagtgca cggacgagtg cctcctgaac
     1981 cggccgtggc aacgacatcg agaggccatt cacatagatt tcgcgtgcgt cgacgccctc
     2041 aggctcgaga tagatctggt ggcgttcccg gtccgggaaa cgcatgatct tgtcctcgag
     2101 cgagggacag tatcgaggtc ctattcctcg aatctggccg ttatagagcg gactctgagc
     2161 gatgttgtct cgaaccaact cgcgcacgcg gtcattggta tgcaggagcc agcaactgat
     2221 gtgattcctg agctggccgg tcgtgcggta cgagaacggc gtcgggtgag gatcgccgcg
     2281 ttcttcgacg aaggttcccg cctccacttg ccgctgaaaa tcaatgctcc tcctgtccaa
     2341 tcgtggcgga gtgccggtct tcaaccgtcc cacttcgaag ccgaggtctc gaatcgattc
     2401 agcgaggctg atcgatggtg gctctccgtg tctgccggcg ggccgctgct ccggcccttc
     2461 gtggatcagg ccgttcagga acgtgccggt cgtcacgacg acagccggac attgaagacg
     2521 ttgcccaccc tcgatcgcca ggcccactat gcgaccgctc tcgacaagca gccgtgaggc
     2581 gggctcaaga atccactcaa tattggggtg ttgcccaatt gcttgccgga tccagtcacc
     2641 atacgtgcgc ttgtcggctt gtgcccgcgg agaccagacc gccggccctc gacttcggtt
     2701 gagcaacttg aactgaattc cggtcgcatc gatggcgcgg cccatcagtc cgcccagtgc
     2761 gtcgatctcc cgaaccaggt gccccttggc ggttcccccg atcgcggggt tgcatggcat
     2821 gtgagcgacc gtcttcagcg agagcgtgca caaccccaca tggcaaccca ggcgcgccgc
     2881 ggcgtgcgcg gcctccacgc ctgcgtgtcc tgcgccgatg acgacgacat cgaacttcaa
     2941 gggcactact tcccgacgca gaagcggtcg aagatgtgcc gcaggacatc ctcattcgtg
     3001 cgtgcgccca cgacttcttc gaaatgatca cgggcctggt gcagatcgac gagaacgaat
     3061 tcttctggcg ctccgtgttc gcccgcggcc gcctgcgcgc gtcgcaatga gtgttgtgcc
     3121 cgccgcagca gcgacgcatg gcgaaggttc gagatcgctg gtgtgtcgcg ccacgcatcc
     3181 ccgccgctca acgccctgac gatcgactgc ttcaaggtgc cgattccgga gttcgtcagc
     3241 gtcgagacgc gaatggcgcc ttcgagatcc caccgtgatc gacgatcgga tttgctcgca
     3301 atcaccagtc gccgtgcgga gggaacgtct tcaagaagcc ggcggtcctc gtcgtccaga
     3361 tcgtctccgg aatcgatgac cacgagaagc aggtctgccg cttcgatcgc gcccatcgcc
     3421 cgattgacgc cctcttgctc tacgacatcg cccgtactgc ggaggccggc cgtgtcgatt
     3481 agcgtgacgg ccaacccgtc gatctccacc tgctcggtga ccaaatctcg cgtagtgccc
     3541 ggaaccccgg tcacgatcgc tcgaggctcg ccgaggagcg cgttgaacaa actcgatttg
     3601 ccgacgttgg ctcgacccac gatcgccact ttcgctccct cacgaacgac gcggccgtgt
     3661 tcggaatgct gcagcacaac ctcgagacag gcgagcactg ccccgatccg gccgccgacc
     3721 tcgtgcggct cgatgaagtg aaacccctcg tccgggaagt cgagcgatgc ctcgagcctt
     3781 gcgatcagat cgagcagttc tgcgtcgagc cgccttacct gatcgctgag cgtgccctcc
     3841 agttgggcga acgcgacccg cgcttgcagt ggcgtgacgg cgtcgacgag atccgccacc
     3901 gcctccgcct gtatcaggtc gaggcgcgag ttcaggtatg cacggaaggt gaattcgcct
     3961 ggtctcgcaa gccgcgcgcc ttcgccaacg aggctctcga gaattgccct gagcaccacg
     4021 gggcttccgt gcgcgctgat ttcgacgacg tgttcgcccg tatatgactg cggcgcggga
     4081 aaccacgtcg cgatgacctg gtcgcccaca cctgaagccg accgatcgag tcgcaccttc
     4141 gtcagcgtcg cgtgacgcgg ttcgagcttc tcccgtcgag cgagaatacg cgtcgcgatg
     4201 gggagggcgt caggtccgct gagccggacg ataccgatac cgccgcgtcc ctctggcgtc
     4261 gcgatggctg cgatcgtgtc gtcggaagaa aacatgcggt cagccgcgac ctcgttgccg
     4321 actgccgggg ccggcgggcc ggttactgct tcgtcgaaat gatgacggtc ttcagaaacg
     4381 cgtcgccgat gctctcggaa ctgacggtcg gatcctcggc aatcgcgaga tgcacgatgc
     4441 ggcgttcgta ggggttcatc ggccccatct cctggggaag gccgctctga cgggctttct
     4501 cggccatgaa ccgcgccatc tgcctgatct cggcatcctt gtccttgcga aacccgttgc
     4561 aatcgatgac gactcggttg tcgtccccca actgccggcg gaacgccgtc gccacgatgt
     4621 gctgcaacgc ctgcagacct tcgccgccac gtcgtatcag caggccgccg tcctcgccct
     4681 cgagattgat ccgagtgccc tcgggcttct cctcgatatt cgcggtcagg ccgacaccca
     4741 tggcgttcac gacgtgctgg acgaagtctg cgatttgcgc ggccaatgtt tggttcacct
     4801 gacgtccctc gtgtgctctt actccgcggc tcgcgtgtgt cgaatccgtc ggcccctggg
     4861 tcagttcttg cgttcggctg ctgccgtgcg cccggttccg gcgctcttca atcggcgctc
     4921 cgcaggcggg cgtgtacccg cggcgaccgg cgggccgatc aaccagttcg tgaagtactg
     4981 ctgaccgatc gcccagaggt ttccgacgaa ccaatagacg acgagaccgc tcggcatcca
     5041 gaggaacatg acgccgaaca tgagcggcat catcatcatg atcttctgct gggcgggatc
     5101 tgcagtcgtc ggcgtgatcc actgctgcca gaacatcgtg gccgccatca gcacgggagt
     5161 gacgtacagc gggtcgtgtt cggacaggtc cttgatccac agtgtgaact cagcgccgcg
     5221 cagctcgatg gcctgtgaga gcagggagta gaacgcgaaa agaatcggga gcgtcaggag
     5281 catcggaacg catccgctgg ccgggttgac gcctttctcc cggtacaggc tcatgatctc
     5341 cgagttcatt ttctggcgcg ccggatcggt caccttgagc cccgcgtagt ggtcctgaat
     5401 cgccttcaat tggggctgta acgcctgcat cttccgcatc gacacgacgc tcttgtgccg
     5461 gaggggcgca atggcaaggt tgatcagaat agtgagcacg acgatcgacc agccccagtt
     5521 gccgacgtag gtgtggaccc acttcaacgc gttcagcaga ggcacagaga gaaacccgaa
     5581 catcccgaag ttaatcgcgc ggacgagctc ctgatcgacc tcacgcagca cctcgaactg
     5641 ctttgggccg atgaagaatc gcaggcgttc cgaggcgttc gacaactgga tcgtctgcgt
     5701 caccaggttg cgctgcctgc tgtcggacac gggcagtgtg agggggtgaa actcgagccg
     5761 cgcctgtccc ggggtgattg ctgcagcaat gaagtaatgg tcgtcgacgc cggcccatcg
     5821 aaaactgcct tcatggacag gttgttcctg cagcgtgctc gccggaaccc tcgaaacttc
     5881 cccgtcgcgg tagtagatcg cctgcggcgg ctggacgtag ttgccagtga agaaactgcc
     5941 gccgccggcc atcgccccgg cgtctccgag gcctggcccc catgcgattc tggtccgcaa
     6001 cgggccggcg ttgttcgaca cagcagcggt gaacgtgacg acgtaattct tgggttcgaa
     6061 cgtgaattct ttccggaccc gcagtccgct tgcgtcctgg aactcgaaga cgagactcgc
     6121 agacgtggtc gccgcatcga ctcggccgtc ggcgtccccg gtcactttgt agagcgcact
     6181 attgagtcgc cgcgtgacgc cctggtcgtc ggccacgatc gaaaacgggc gtggctgatc
     6241 ggctggcacg t
//
LOCUS       DBNGSH010001052         3400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2540513, whole genome shotgun sequence.
ACCESSION   DBNGSH010001052 DBNGSH010000000
VERSION     DBNGSH010001052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2540513"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1511
                     /locus_tag="AC16CY_23835"
     CDS             54..1511
                     /locus_tag="AC16CY_23835"
                     /inference="COORDINATES: protein motif:HMM:NF020390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HIR3356905.1"
                     /translation="MARGQPGVEHLLRRAGFGASQDDLKVFEDMSTFGALEHLLDYER
                     QPDDVDSKIGNPAYAAVPAGLSPNLNIEHARLRWLFRMVHSNRPLQEKMALFWHNHFA
                     TAYSKIAGSFVGPGQATRMMANKPGELSGPPGQYELFRSRALGSFRDLLIDVAKDPAM
                     LVWLDGILNTRVRPQENFAREIMELFTFGIGHYTELDVYAAARVFTGWNIRPVGNRAA
                     GDAAFYYEFVYNGSQHDTGEKTFTFPIYRGGGRTIPARPPAEGMQDGIDFMTALATHP
                     ATARRLARKLWTFFVNEVEEPDPSLIGDAATIYLQSGTRIGPVVSYMLRSREFQNPGN
                     WHTRYSWPVEFVARAIKEVGWVGYNLASARTPLLSMGQLLFEPPDVNGWELGQGWFTT
                     GGMLARMNFAAGLAGTQRLNLASAAASAGSSPESLLEYFMERLSPLPYAESPRTELMA
                     YLESGGAWTGSEAQLRLKATGLARLIVGSAEYQLV"
     gene            1573..2832
                     /locus_tag="AC16CY_23840"
     CDS             1573..2832
                     /locus_tag="AC16CY_23840"
                     /inference="COORDINATES: protein motif:HMM:NF019040.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3356906.1"
                     /translation="MVTRRDFIRDGAAAFVVSFAAPAFLGDAARAQGARSRNLVVVYL
                     GGGNDSLNTVVPYQDEYYYRRRPTIAVPAGQVLQIGQDSGGNQLGLHPRLSGLHGIFN
                     EGRLAIVQRTGYTGSSRSHFQGLDIWSTAKPSEPTTTGWLGRFLETVQPPHGLCGWCT
                     TRAVPRALLSRELSVPGISDARAYRFESPNGVAEGLNERRAASRIASHVAVDRPHLAF
                     VNAAATSTFETLDVVAQVAAYVPTVAYPGGGFGLALQTVAGSIVRNTGTRVYWVQTGG
                     FDTHAAQGGQGGGFYGNLMGTYNDGITAFYNDLRNHGLLGDTLVLQFSEFGRRIAENG
                     SAGTDHGAAAVMMAIGGAVRGGVYGTAPSLNNTDSSNPTLESNGGDVRHETDFRAVYA
                     RVLDGWLGASSTAILGGDFRAGAPPVV"
     gene            complement(2896..>3400)
                     /locus_tag="AC16CY_23845"
     CDS             complement(2896..>3400)
                     /locus_tag="AC16CY_23845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356907.1"
                     /translation="ASAGRVRLHRQIGVAGMAYGAFVFLIGVMVSIGAPALRVRAGDF
                     PVEVGGMVAILSLADLVLFGAFLAFAFAYRSQPEVHKQWVVAATAALGGAAVGRVLKS
                     DSLAYLLVWLSPVLAIIAIDLASRRRLSAVPFVSGGLIVVSFFKAQLLAAPIWGEFGR
                     ALVRPFV"
BASE COUNT          623 a         1139 c         1116 g          522 t
ORIGIN      
        1 ggagtgtaag tcgaggcgcg aaaacccacc gtctctcccc tggaggacgc gtcatggcac
       61 gaggacaacc cggagtcgaa cacctgctcc gacgcgccgg cttcggcgcg agccaggacg
      121 acctgaaagt cttcgaagac atgtccacgt ttggcgctct cgagcacctg ctcgactacg
      181 agcggcaacc cgacgacgtg gactcgaaga tcggcaaccc cgcctacgcc gccgttcccg
      241 ccgggttgtc accgaacctg aacatcgagc acgcgcgcct gcgctggctg ttccgcatgg
      301 tgcatagcaa ccgccctctg caagagaaga tggcgctctt ctggcacaac cacttcgcca
      361 ccgcgtacag caagatcgcc ggcagcttcg tggggccagg acaggccacc cggatgatgg
      421 cgaacaagcc gggcgagctg tcggggccgc caggccagta cgagctgttc cggtcgcgcg
      481 cgctcggaag cttccgcgac ctgttgatcg acgtcgcgaa agatccggcg atgctcgtgt
      541 ggctcgacgg catcctcaac acgcgcgtgc gtccgcagga gaacttcgcg cgcgaaatca
      601 tggagttgtt cacgttcggc atcggccact acaccgagct cgacgtgtac gccgcggcgc
      661 gcgtgttcac cgggtggaac atccgtccgg tcggcaaccg ggctgcaggc gacgcggcct
      721 tctactacga gttcgtctac aacgggagcc agcacgacac gggggaaaaa accttcacgt
      781 tcccgatcta tcgcggcggt ggccggacga tcccggcgcg tccgccggcc gaggggatgc
      841 aggacggcat tgatttcatg acggcgctgg cgacgcatcc ggcgacggcg cgcaggctgg
      901 cgcgaaagct ctggacgttc ttcgtgaacg aggtggagga gccggacccg tccctcatcg
      961 gcgacgcggc caccatctac ctgcagagcg gcacccgcat cggaccggtc gtgtcgtaca
     1021 tgctgcggtc ccgcgagttc cagaaccctg gcaactggca cacgcgctac tcctggcccg
     1081 tcgagttcgt cgcccgcgcg atcaaggaag ttggttgggt cggctacaac ctggcctccg
     1141 cgcgcacgcc gctcctgtcg atgggccagc tcctgttcga gccgcccgac gtcaacgggt
     1201 gggagctcgg acagggatgg ttcacgaccg gcggcatgct cgcgcgcatg aactttgccg
     1261 cgggccttgc aggcactcag cggctcaacc tggcaagcgc cgctgccagc gcgggctcct
     1321 cgcccgagtc gctgctggag tacttcatgg agcggctgtc gccgttgccc tatgccgaat
     1381 ctccacgcac ggaactgatg gcgtacctcg agagcggagg cgcgtggacc ggaagcgagg
     1441 cgcagcttcg cctcaaggca acagggttgg cgcggctgat cgtgggatct gcggagtatc
     1501 aactggtcta agcagggggc agggggcagg gggcaggggg cagggggcag accgaagacc
     1561 ggagaccgac atatggttac tcgccgagac ttcatccgtg acggtgcggc ggcgttcgtg
     1621 gtcagcttcg cggctccggc gtttctgggc gacgccgcac gagcgcaggg tgcgcgctcg
     1681 cgaaacctgg ttgtcgtcta cctgggcggc ggcaacgatt ccctgaatac cgtcgtgccg
     1741 taccaggatg agtactacta tcgccggcgc ccgaccatcg ccgtgcccgc ggggcaggtg
     1801 cttcaaatcg gacaggattc cggtggcaac cagctcggat tgcacccgcg actctccgga
     1861 ctgcacggca tcttcaacga aggacgtctc gccatcgtcc agcgaaccgg atatacgggc
     1921 tccagccgat cccactttca gggactcgat atctggagca cggccaaacc gtcggagccg
     1981 accaccacgg ggtggctggg ccgcttcctc gaaaccgtgc agccgccaca cggcttgtgt
     2041 ggctggtgta cgacgcgcgc ggtgccgcgc gcgctgctct cgcgcgagtt gagcgtcccg
     2101 ggaatcagtg acgcgcgagc gtaccggttc gaaagcccca acggcgtggc cgaaggactc
     2161 aacgaacgtc gcgcggcctc gcggattgcg tcgcacgtcg cagtggaccg cccgcatctg
     2221 gcgtttgtca acgccgcagc gacgagcacg ttcgagacgc tggacgtggt ggcgcaggtg
     2281 gccgcgtacg tccccacggt ggcgtatccc ggtggcggct ttggcctggc gctgcagacg
     2341 gtcgccggct ccatcgtccg caacacgggg acgcgcgtgt actgggtgca gaccggcggc
     2401 ttcgacacgc atgctgcgca gggcgggcag ggcggggggt tttacggcaa cctgatgggg
     2461 acctacaacg acggcatcac cgctttctat aacgacctgc gcaaccacgg cctgctgggc
     2521 gacacgctcg ttctgcagtt ctcggagttc ggcaggcgca tcgccgagaa cggaagcgcc
     2581 ggcaccgatc atggcgccgc ggcggtgatg atggcgattg gaggcgcggt gcgtggcggc
     2641 gtgtacggga ccgccccgtc actgaacaac acggactcca gcaacccgac gctcgagagc
     2701 aacgggggcg acgtccggca cgagaccgac ttccgcgccg tctacgcccg cgtgctcgac
     2761 ggctggctcg gcgcgagctc cacggcgatc ctcggcggtg acttccgggc cggagccccg
     2821 ccagtcgtct gagcgagtac cccctcgcac cgtcgcaccc tcgcaccttt accgtcgtct
     2881 ttcggccaga agccgtcata cgaacggacg gaccagtgcc cggccgaatt caccccagat
     2941 cggcgccgca agcagttgcg ctttgaaaaa cgagacgaca atcagtcccc cgctcacgaa
     3001 cgggacggca ctcaacctgc gccgagacgc gagatcgatc gcgatgatcg ccagcacggg
     3061 agagagccac accaacaagt acgccagcga gtcactcttg agtacgcgtc caacggccgc
     3121 accgccaagg gccgccgttg cggcgacaac ccattgttta tgtacttcgg gctgcgaacg
     3181 atacgcgaag gcgaaggcaa ggaacgcgcc gaaaagcacc aagtcggcga ggctgagaat
     3241 cgccaccatg cctccgactt cgaccgggaa atctccagcc cgcacacgaa gcgccggcgc
     3301 cccgatactg accatgacac cgatgagaaa aacgaacgca ccgtacgcca ttccagccac
     3361 accgatctgt cggtgtaagc gcacgcgccc ggcactcgcg
//
LOCUS       DBNGSH010001053         4557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4749059, whole genome shotgun sequence.
ACCESSION   DBNGSH010001053 DBNGSH010000000
VERSION     DBNGSH010001053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4749059"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1465
                     /locus_tag="AC16CY_23850"
     CDS             104..1465
                     /locus_tag="AC16CY_23850"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3356908.1"
                     /translation="MKRRSLPPRGAPIPIWAVAAIVTAACACATSGQAPDRAAIDSAI
                     RERTSSGIRIESTAPSMPPDVTLDDGLTSDEAVAIALWNSPSFQATLADLGVARADLV
                     EAGLLRNPVLSLLFPLGPKQLEWTVQFPFELIWQRPARVAAASLNAQAVGERLVWNAL
                     TLVAETRTAHGDAVIADRRLQLTSEIADLVRRLAGITEARLRAGDISELEARAARSDA
                     ARVEAVRQAVEHDRDLSRLTLAALLGLDADADRIRPAPGPAPDVAACGTDAARVERAL
                     ASRPDVRAAELGIDAAAQRARWERSRVVTLMGILDGNGEGAEGAEVGPGIGAEIPIFN
                     RNQGGIRRAEAEVERASHRYAAVRAQVVAEVRAASVRVRQAQQAIAAWQKDIVPSLEV
                     EQRQAESAYQAGEIPLFSLLDVSRRLVDGRTRHLDAEADLHRAAIALERSMGRSCLER
                     GQP"
     gene            1561..2700
                     /locus_tag="AC16CY_23855"
     CDS             1561..2700
                     /locus_tag="AC16CY_23855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3356909.1"
                     /translation="MSFAAAGCSGGGPSGGAHEAPASVEKPQQEADLTTVKLTQDAVN
                     RLGLRTVKAATESAALTRTLGGAIVVPEGRGVVVTAPVAGTVMDASGARPGVRVRRGE
                     SLMTIAPLVSTERDQRIEAQRGVSSAEAEELAARQRLQRLEQLLKDGATSMRAVEEAR
                     AQHQVSVATLTAARDRLAGASRNPVGAQGDLVVSAPFDGVVQRVAAVQGQTVAASAAL
                     LEIAQMDSLWVRVPVYAGIVDDIDTGRPVSIQRLGASSAPMRGTYVAAPLSGDPAAAS
                     VDLFFALERAGSSLRPGERVLVELSLRKNERALVVPSSAVLYDVHGDAWVYEDLGGRA
                     YARRRIQVARFAGDRAIVTRGIAEGTPVVTDGAAELFGTEFGAGH"
     gene            2705..>4557
                     /locus_tag="AC16CY_23860"
     CDS             2705..>4557
                     /locus_tag="AC16CY_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3356910.1"
                     /translation="MWWIVRLSLHHRVVVVALGVLLVVLAARTLGTTPLDVFPEFAPP
                     RVEVQTEAPGLSTDEVESLVTVPLEAALNGVPGLSTIRSKSVLGLSSVVLILDPSADL
                     MSARQVVQERLARVTTQLPAVAHPPVMLAPLSSLSRVMKIGMTSETLTQVELSTLARW
                     TIRPRLMAIPGVANVAIWGQRDRQLQVLVDPERLRANGVTVDDVLRATGEAVSVQAGG
                     FLDTPNQRLAVTHAGAVRSATDLESIVIVARNGAALRVGDVATVVEGFPAPIGDAVIN
                     NGPGLMLIVEKQLGANTLSVTREVERTLDEMKPALAGVAVDPTIFRPATFIEMSLQNL
                     SRALLVGCVLVVLVLLVFLADWRTALISSIAIPLSLLAAGLLLHYRGGTLDTMVLAGL
                     VIALGEVVDDAIIDVENIMRRLRLNRVAEHPRSPLSVVLDASMEVRSAVLYGSLIVVV
                     VFVPVFMLDGLAGAFFRPLALSYVLAILASLLVALTITPALALLLLPRRLDGREARLV
                     TSLKARYRQVLPALVDRPKAALGSLAAMFALTAGVAPLLGEEFLPNFREYDFLMHWVE
                     KPGTSIEAMRRITERASRELMAVPGVRNFGAHIGRAEVADEVVGPNFTELW"
BASE COUNT          641 a         1585 c         1615 g          716 t
ORIGIN      
        1 gacagtatcg cgacgagctg cagccgttga ccggcagccc gctcgacgag cgcgtctaca
       61 cgatcgttca gtagtccggc gaaatagcct atccttcccg gtagtgaagc gtcgttcact
      121 cccgccccgc ggggctccta ttccaatatg ggcggtcgcg gcgatcgtca cggcggcctg
      181 cgcgtgcgcc acgagcggcc aggctcccga tcgggcggcg atcgactcgg cgatccgcga
      241 gcgcacgagc agcgggatcc ggatcgagag cactgcgccg tcgatgccgc ccgacgtcac
      301 gctcgacgac gggctgacgt cggacgaggc ggtggcgatc gcgctctgga acagcccgtc
      361 gttccaggcg acgctcgcgg acctcggagt cgcccgcgcg gatctcgtgg aagccgggct
      421 gctccggaat cccgtgttgt cgttgctgtt tcctcttggc cccaaacagc tcgaatggac
      481 ggtgcagttc cccttcgagc tgatctggca gcgccccgcg cgcgtcgccg ccgcgtcgtt
      541 gaacgcgcag gccgtcggcg agcgcctggt gtggaatgcg ctgacgctgg tggccgagac
      601 gcgcaccgcc catggcgacg cggtcatcgc cgatcggcgg ctgcagctga catccgagat
      661 cgcggacctc gtccgccggc tggccggcat cactgaggca cgtctgcgcg cgggcgacat
      721 cagcgagctc gaggcgcgcg ccgccagaag cgatgcggcg cgagtcgagg ccgtgcgcca
      781 ggcggtcgag cacgatcggg atctgtcgcg gctgacgctc gccgcgctcc tcggactcga
      841 cgcggacgcc gatcggattc gtccggcacc gggtcccgct cccgacgtcg cggcgtgcgg
      901 caccgacgcc gccagggtcg agcgggcgct ggcgtctcgc cccgacgttc gcgccgccga
      961 actgggcatc gacgccgcgg cccagcgcgc gcgctgggag cgatcgcgcg tggtgacgct
     1021 catgggcatc ctcgacggaa acggcgaagg cgctgaaggg gccgaggtgg gacccggcat
     1081 cggcgccgag atcccgatct tcaatcgcaa ccagggcggc atacgacggg cggaggccga
     1141 agtggagcgc gccagccatc gatacgcggc ggtgcgcgcc caggtcgtcg ccgaagtccg
     1201 ggcggcctcg gtcagggtgc gtcaggccca gcaggccatc gccgcatggc agaaggacat
     1261 cgtgccgtcg ctcgaagtgg aacagcgtca ggccgagagc gcataccagg cgggcgagat
     1321 tccgctcttt tcgctcctcg acgtgagccg ccggctcgtg gacgggcgca cccgccacct
     1381 cgatgccgag gccgatcttc acagggccgc catcgcactg gaacggagta tgggccgctc
     1441 gtgcctcgaa cgcggacaac catgatgccg attcgatacg aaccacggag acgcggagga
     1501 cacggagaat ccctattgtc gtctccgtgt ctctgtgcct ccgtggtgta cgtgatcgcc
     1561 gtgtctttcg ccgctgcggg gtgctccggc ggcgggccgt ccggcggggc tcacgaagcg
     1621 ccggccagtg tcgagaaacc gcagcaggag gcggacctga ccacggtgaa gctgacccag
     1681 gacgccgtca atcggctcgg gctgcgcacc gtgaaagccg ccaccgaatc ggccgccctg
     1741 acgcgcacgc tcggcggcgc catcgtcgtc cctgaagggc gcggcgtcgt cgtgaccgcg
     1801 cccgtcgcgg ggacggtcat ggacgcctcc ggcgcacggc cgggggtgcg ggtgcggcgg
     1861 ggcgagtccc tgatgacgat cgcaccgctg gtttcgaccg agcgcgatca gcggatcgag
     1921 gcgcagcggg gcgtcagctc ggccgaggcc gaagaactgg ccgcgcgcca gcgtctgcag
     1981 cgcctcgagc aactgctcaa ggacggcgcg accagcatgc gggccgtcga ggaggcgcgc
     2041 gcgcagcacc aggtgagcgt cgccacgctg acggccgcgc gcgatcggct ggcaggcgcg
     2101 tcgcgaaacc ccgttggcgc gcagggcgac ctcgtcgtgt cggcgccctt cgacggcgtg
     2161 gtgcagcgcg tggccgcggt gcagggccag acggtggcgg cctccgcggc cctgctcgag
     2221 attgcgcaaa tggactcgtt gtgggtccgc gttcccgtgt atgccgggat cgtggacgac
     2281 atcgacaccg gcagaccggt ctcgatccag aggctcggcg cctcgtccgc gcctatgagg
     2341 gggacgtacg tcgccgcgcc gctctcgggc gaccccgccg ccgcgtccgt cgatctgttc
     2401 ttcgcactcg aacgcgccgg gtcgtcgctc cgtcctggtg agcgggtgct ggtcgagctc
     2461 tcgctcagga aaaacgagcg cgcgctggtc gttccgtcga gcgccgtgct ctacgacgtc
     2521 cacggcgacg cgtgggtgta tgaggatctc ggaggacgcg catacgcacg gcggcgcatt
     2581 caggtggcgc gattcgccgg ggatcgcgcc atcgtcacgc gcggcatcgc cgagggcact
     2641 ccggtcgtga ccgacggggc cgccgagctc ttcgggacgg aattcggcgc cggacactga
     2701 cgccatgtgg tggatcgtcc ggctgtcgct tcaccaccgc gtcgtcgtcg tcgcgctcgg
     2761 ggtgctgctc gtcgtgttgg cggcgcggac gctcggcacg acgccgctcg acgtcttccc
     2821 tgaattcgct cccccgcgtg tcgaggtgca gacggaagct ccaggcctct cgaccgacga
     2881 ggtcgaatcg ctcgtcacgg tcccgctgga ggcggcgctc aacggcgtgc ccgggctcag
     2941 cacgatccgc tcgaagtccg tgctcggcct ctcctctgtg gtgctgatcc tggacccgtc
     3001 cgcggacctg atgtcggccc gccaggtggt gcaggagcgg ctcgcgcgcg tgacgacaca
     3061 gctgccggcg gtggcccacc ccccggtgat gctggcgccg ctgtcgtcac tcagccgggt
     3121 catgaagatc ggcatgacgt ccgagacgct cacgcaagtc gagctgagca cgctcgcgag
     3181 gtggacgatc aggccgaggc tgatggctat tcccggtgtg gccaacgtcg ccatctgggg
     3241 gcagcgcgat cgccagcttc aggtcctggt ggacccggag cgtctccgcg ccaacggcgt
     3301 cacggtggac gacgtcctgc gcgccaccgg tgaggccgtg tcggtccaag ccggcgggtt
     3361 cctcgacacg cccaaccagc ggctcgcggt gacgcacgcc ggcgccgtcc gatcggcgac
     3421 ggatctcgag tcgatcgtca tcgttgcgcg caacggcgcc gcgctccgcg tgggcgacgt
     3481 ggcaacggtg gtcgagggct tccccgcccc catcggcgat gccgtgatca acaacggccc
     3541 gggcctgatg ctgatcgtcg agaagcagct cggggcgaac acgctcagcg tcacgcgcga
     3601 ggtcgagagg acactcgacg agatgaagcc cgccctcgcc ggagtggccg tggacccgac
     3661 cattttccgg ccggccacct tcatcgagat gtcgctgcag aacctgagcc gggcgctgct
     3721 cgtcggctgc gtgctggtcg tgctcgtgct gctggtgttc ctggccgact ggcgcaccgc
     3781 gctcatcagc agcatcgcca tcccgctgtc gctgctcgcc gcgggcctcc tgctccacta
     3841 ccgcggcggc acgctcgaca cgatggtcct ggccggcctc gtcatcgcgc tcggcgaggt
     3901 cgttgacgac gccatcatcg acgtcgagaa catcatgcgc cggctgcgtc tcaaccgtgt
     3961 ggcggaacat ccacgatctc cgctctcggt ggtgctcgat gcctcgatgg aagtcagaag
     4021 cgccgtgctc tacggcagcc tgatcgtcgt cgtcgtgttc gtcccggtgt tcatgctcga
     4081 cggcctcgcc ggggccttct tccggccgct ggcgctctcc tacgtcctcg cgatcctggc
     4141 gtcgctgctc gtcgcgctga cgattacgcc ggcgctggcg ctgctgctcc tgccccgccg
     4201 cctcgacggc cgcgaagccc gtctcgtcac gagcctgaag gctcgatatc ggcaagtgct
     4261 tcccgcgctg gtggaccggc cgaaggcagc actcggctcg ctggcggcga tgttcgcgct
     4321 gaccgcgggg gtggcaccgc tgctgggcga ggaattcctt cccaacttcc gggagtacga
     4381 cttcctcatg cactgggtgg agaagcccgg cacgtccatc gaggccatgc ggcgcatcac
     4441 cgagcgcgcg agccgggagc tgatggccgt tccgggcgtt cgaaacttcg gcgcgcacat
     4501 cgggcgcgcg gaagtcgccg acgaagtggt cggccccaac ttcacggagc tctggat
//
LOCUS       DBNGSH010001054         5318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3390460, whole genome shotgun sequence.
ACCESSION   DBNGSH010001054 DBNGSH010000000
VERSION     DBNGSH010001054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3390460"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="AC16CY_23865"
     CDS             <1..732
                     /locus_tag="AC16CY_23865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356911.1"
                     /translation="VWGVAVHPVSVTAGASGAIFGLYGLLIATLLWGLVERSAATIPF
                     AALKGLWPGVAIFLVYHVLTEGFVSSSMQAGLVVGFVCGAVLTGRVISHKPPVRRVCA
                     ALAATVGIVVICAAPLRGLADINGEVALVKQMETRTATAYDSAVGRFRKGRIDAGELA
                     ALAEGIASEMEVLRTHLASISNVPRDHQSLFTAISEYVQLREDSWRLRAEGLRAGQMA
                     TLQKADSAQRAALTALERTDASEQN"
     gene            complement(883..1893)
                     /locus_tag="AC16CY_23870"
     CDS             complement(883..1893)
                     /locus_tag="AC16CY_23870"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HIR3356912.1"
                     /translation="MCCFQVGCSLGVPWEEWELPHNRSSARRPRVKLHRNAKTTPLTR
                     ALLVSRVRHQGWTPAAAAVAHGVSVRTVFKWLRRGREATFEDRPSAPHRQPRRTSAVM
                     TAAIVGARYEGLTAWRIAVRLQVPRSTVSAVLVRCGLNRLARLQPRPPIHRYERSRPG
                     ELVHLDMKRVARIGVVGHRIHGDWRRRTRGLGWETIHVCVDDFSRVAYVEVLPDQHGA
                     TAAAFLHRASQWFARRGVRLERVLTDNGPAYYSHRFRQRCAALAVRHLRTRPYRPQTN
                     GKAERFIQTLLREWAYGATYPSSWRRTRALRPWLRFYNAERPHASLAYRPPVIRFPRA
                     AQ"
     gene            complement(1964..3493)
                     /locus_tag="AC16CY_23875"
     CDS             complement(1964..3493)
                     /locus_tag="AC16CY_23875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIR3356913.1"
                     /translation="MSVVTPHLKVSLPRRRAITAGLLLGMSLGALEATVVSTAMPTVI
                     ATLGGLAHYGWVFSAYLLTSTASVPIWGRLSDLYGRRRMYLIGVAVFLVGSVACGTAT
                     NMITLIIARLIQGLGAGAIIPMSMTIVGELYTLAERARTQALFSGVWGVASIAGPLVG
                     GYITDALSWRWVFYLNVPFGFCCMAVIALAYPASRLTRLVHVDWLGASLMFLGISSLL
                     LALGGDAGSSAWWLAAAVALLGGFVATERRSREPMLPIQLVSMPVISRSIVVVFLAGI
                     AMFGAIAFIPLFVQSVQGGNATQAGQVLTPLFLGWVVMSIVGARVTVRVGYRVAAVGG
                     SILMTVGFVGLSLLGSDSPRMLLLASCTVLGAGMGSQMLSMLLAVQHAVQRSQLGLAT
                     SLTQFSRSVGAAIGVAAMGAIMTRSLTGISLPGGAEGLAASGAMALSGPVRDQFAAAL
                     HRVFLAGTVVSIAGLVASLFLPHVDLSHGMPASAGEQMIEAEMTNLRPEDEPVAVPEE
                     A"
     gene            complement(3579..4475)
                     /locus_tag="AC16CY_23880"
     CDS             complement(3579..4475)
                     /locus_tag="AC16CY_23880"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3356914.1"
                     /translation="MTLHTRTTLAMACIAAGMLSLPATAAAQHHGPVIQSRPAVSVGV
                     GVYYGPTVYRSWYASPYFYWGGYPFYPYYGAPWYPYPYAYGYPYAYPYAYNVGGSLRL
                     QVTPKQAGVFVDGYYAGTVDDFDGTFQRLHVEPGEHDLQVYLPGHRLIRQKVYLQPGK
                     TFSVRQAMEPLAPGEVEPEPPVPAEKPADARPQQSRTRPGRPPARSRDQVDVEPEPAP
                     PSPSRPEFGAISLRVQPDDASITIDGERWENAPVNERLIVQLGTGVHNVEIRRDGYRT
                     YVTDITVRPGQTATINVSLTKQ"
BASE COUNT          943 a         1775 c         1770 g          830 t
ORIGIN      
        1 gtctggggtg ttgccgtgca ccccgtgtcg gtgaccgcgg gtgcgtcggg agcgatcttc
       61 ggcctgtacg ggttgctgat cgccacgctg ctctgggggc tcgtggagcg ttcggcagcg
      121 acgatcccgt ttgcggcgct gaagggcctc tggcctggcg tcgcgatctt cctcgtgtac
      181 cacgtcctga ccgagggatt cgtcagctcg tcgatgcagg ccgggctcgt cgtggggttc
      241 gtctgcggag ccgtgctgac cggccgggtc atttcgcaca aaccgccggt ccggcgggtg
      301 tgcgcggcgc tggcggcgac ggtcggcatc gtcgtcatct gcgccgcacc gcttcgcggg
      361 cttgccgaca tcaacggaga ggtcgcgctc gtcaagcaga tggaaacgcg caccgccacg
      421 gcctacgact cggcagttgg tcggttcagg aaggggcgca tcgatgccgg ggagctggcc
      481 gctctcgccg agggtatcgc gtcggagatg gaagtcctga ggacccatct ggcgtcgatc
      541 agcaacgtgc cccgcgacca tcagtcgctg tttacggcga tctcggaata cgtgcagctg
      601 cgcgaagaca gctggcggct gcgggccgag gggctgcgcg ccggacagat ggcgacgctg
      661 cagaaggccg acagcgcgca gcgggcggcg ctgacggcgc tcgagcgaac cgacgcttcc
      721 gaacagaact agttagtcct tggtccttgg ttcttggtcc ttgggtccgt tcttcgtcct
      781 gggtccgacc ctggtccccg gtcctgggtc catgacggac gtaggaccaa ggacggacat
      841 aggaccaagg gcggacatag gtgttgtttc tagggacgtt gttcactgtg ctgcccttgg
      901 gaagcggata acgggcggcc gatatgcgag gctcgcatgc ggccgctctg cattgtaaaa
      961 ccgcagccac ggccgcaacg cgcgggtgcg tcgccacgag ctgggatacg tcgccccgta
     1021 cgcccactcc cgtaacagcg tctgaatgaa ccgctcggcc ttgccattcg tttgcggacg
     1081 gtacgggcgc gtacggagat ggcgcacggc cagcgcggca cagcgctgcc ggaagcgatg
     1141 tgagtaatac gccggcccgt tatcggtcag cacccgctcg aggcggacgc cgcgccgcgc
     1201 aaaccattga gaggcccggt ggagaaacgc ggcggccgtc gcaccatgct gatccggcag
     1261 gacttcgacg taagcgacgc ggctgaaatc atccacgcaa acgtggatcg tctcccaacc
     1321 taatccgcgg gtccggcgtc gccaatcccc atggatgcgg tggccgacca cgccgattcg
     1381 cgcgacccgt ttcatatcga gatggaccaa ctcccccggc ctcgaccgtt catagcggtg
     1441 gatcggcggc cggggctgca ggcgggcaag ccgattgagc ccacaccgca ccagcaccgc
     1501 cgacacggtt gagcggggca cttgcaggcg cacggcaatg cgccaggcgg tgaggccctc
     1561 gtaacgagca ccaacgatcg cggcggtcat gacggcgctt gtccgacgcg gctgccgatg
     1621 tggcgccgac ggacgatcct caaacgtggc ctcacggccg cggcgcagcc acttgaacac
     1681 cgtgcgcacg ctgacgccat gcgccaccgc ggccgcagcc ggcgtccaac cttggtgccg
     1741 cacgcggctt accaggagcg cgcgggtcaa cggagtcgtc ttggcgttac gatgaagctt
     1801 cactcggggc ctcctcgctg aagaacggtt gtgtggtaac tcccattctt cccaagggac
     1861 tccgagtgaa caacctacct ggaaacaaca cataggacca aggacggacc gaggaccaag
     1921 cacggaccaa gccaccaagg accaaggaca aaggaccgtt acttcatgcc tcttcaggca
     1981 ccgccacagg ctcgtcctcc ggcctgaggt tggtcatctc ggcttcgatc atctgctcac
     2041 cagccgaggc cggcattccg tgggagagat cgacgtgcgg cagaaacagc gaggcgacga
     2101 ggcccgcgat cgacacgacg gtgccggcga ggaacacgcg gtgaagggca gcagcgaact
     2161 ggtcgcgcac cgggcccgac agcgccatcg ctcccgacgc ggcgagccct tcggcgccgc
     2221 caggcagcga tatcccggtc aggctgcgcg tcatgatcgc gcccatcgcg gcaaccccga
     2281 tggccgcccc gacgctgcgc gagaactggg tgagcgacgt cgcgaggccg agctgcgacc
     2341 gttgcacggc gtgctgcacg gccagcagca tcgacagcat ctggcttccc attccggcgc
     2401 cgagcacggt gcagcttgcg agcagcagca tgcgcggtga gtcgctgccg agcaggctga
     2461 gccccacgaa ccccaccgtc atcagaatgc tcccgccgac cgccgccacg cgatagccca
     2521 cgcgaacggt gacgcgggcg ccgacgatcg acatcacgac ccagccgagg aagagcggcg
     2581 tcaacacctg gcccgcctgc gtggcgttgc ctccctgtac gctctgcacg aagagaggaa
     2641 tgaaggcgat ggcgccgaac atggcgatgc ccgccaggaa gacgacgacg atggagcgcg
     2701 agatgacggg catcgacaca agctgaatcg gaagcatcgg ttccctggaa cggcgttcgg
     2761 tcgccacgaa accgccaagc agcgccaccg cggccgccag ccaccaggcc gacgaacccg
     2821 cgtcgccgcc gagcgcgagg aggagcgaac tgatccccag gaacatcaac gaggcgccga
     2881 gccagtccac gtgcacgagg cgcgtcagcc gcgacgccgg gtaggcgagc gcgatgacgg
     2941 ccatgcagca gaatccgaac gggacattca ggtagaacac ccaccgccac gagagggcgt
     3001 cagtgatgta gccgcccacg agcggtccgg cgatcgacgc cacgccccac acgccgctga
     3061 agagcgcctg ggtcctggcg cgctcggcga gcgtgtacag ctccccgacg attgtcatgc
     3121 tcatcgggat gatcgcgccg gcgccgagtc cctgaatcaa cctggcgatg atgagcgtga
     3181 tcatgttcgt cgcggtgcca cacgccaccg agccgacgag gaagacggcc acgccaatca
     3241 ggtacatccg ccggcggccg tagaggtcgg agagccggcc ccagatcggg accgatgccg
     3301 tagacgtcag cagatacgcc gagaacaccc agccgtaatg cgccagcccg ccaagggtcg
     3361 cgatgacggt cggcatcgcg gtgctgacca ccgtcgcctc gagcgcgccg aggctcatgc
     3421 cgagaagcag accggccgtg atcgcgcgcc ggcgaggaag actgaccttc aaatgtggag
     3481 tcacaacgga cattgatagc agagtaccac ggcacacgag cgagcggtcc ctcgcctccc
     3541 gcgactctgc aggtgccgcc gtgcctgcct gctaccggct actgcttcgt gagtgaaacg
     3601 ttgatcgtcg ccgtctggcc cggacgcacg gtgatgtccg tgacgtaggt acggtaaccg
     3661 tccctccgga tctccacgtt gtgcacgccc gtgcccagct gcacgatcag gcgttcgttc
     3721 accggagcgt tctcccaccg ctcgccgtcg atcgtgatcg aggcgtcgtc tggctgcact
     3781 cgcagcgaga tcgcaccgaa ctcgggcctc gatggagacg gcggcgcagg ttccggttcc
     3841 acgtcaacct gatcgcgcga gcgggccgga ggccggcctg gccgcgtgcg ggattgctgc
     3901 ggcctggcgt ccgccggttt ctcggccggg accggaggct cgggctcgac ttctccaggg
     3961 gcaagcggct ccatcgcctg gcgcacactg aacgtcttgc ccggttgcaa gtacaccttc
     4021 tgccggatca gtcgatgtcc gggcaggtac acctgcagat cgtgttcgcc gggttcgacg
     4081 tgcagccgct ggaacgtgcc gtcgaagtcg tcaaccgttc cggcgtagta gccgtccacg
     4141 aacacgcccg cctgcttcgg cgtgacctgc aggcgcagcg atccgccaac gttgtacgca
     4201 taagggtacg cgtacggata tccatacgcg tacggatacg ggtaccacgg cgccccgtag
     4261 tagggataga acgggtagcc gccccagtag aaatacgggc tcgcgtacca cgaacggtag
     4321 acggtggggc cgtagtagac gccgacgcca acgctcaccg caggacgcga ttggatgacg
     4381 ggcccgtggt gctgagccgc ggcggtcgcg ggaagtgaca gcatgccggc ggcgatgcac
     4441 gccatggcga gagtggttcg cgtatgcagg gtcacagtgg ccctccctcg agtgctacca
     4501 gtgtgcactg gacgcaacgg tcgtgccgct cgaaacccgt cgtcagtcgc ggaaaatcgc
     4561 cgtgtgcacc ggatgtgtgc gtcgctcggc gtggacgatt gtcacggctg ggtgacggga
     4621 ggtgtctgct cacctcggcc tggaggcgga ccctcgcctc tgccacgccg gcccgacccg
     4681 tcacctcgac ctcgccggcc cgatccatcg ccgcgtccgc gccggcctgt tccatcgcct
     4741 cgtgctcggc cctcacctgt gccgcggcgc ccggtcccat cgcctcgtgc gcggccctcg
     4801 cctgcgccgc ggcgcccggt cccatcgcct cgtgcgcggc cctcgcccgt tcctctcccg
     4861 gtgttctgat ccggaggctt ctcgccctcg ggtgcctgcg ccagggttgg ctttgccgga
     4921 acggcaaatg caagtgcggt ccctgctgct gcaagcaggt gtttcaggag cgcgcggcgt
     4981 gcagggtcga cggttcgagg aaccacgtca tcctcctcag cagtgcgcat agttttacat
     5041 acttaacacg gggattgtag aacgaccagt acgggcatgt gatgattgtc ggggccagac
     5101 tagcggactg tatcaactgg aagagtaccg cgaacaacga gcgccgaccg cgatcatccg
     5161 gccgcgacaa cgcccctcga cttcgctcgg ggcgtcctga gcgagagtcg aaggacggag
     5221 cggccggtag taattcgcga aggacatacc actagattca tgcagatccg atcgacatca
     5281 cgtcttcgcc agatcgcggg aactgccgcg agcctcgt
//
LOCUS       DBNGSH010001055         2904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4579503, whole genome shotgun sequence.
ACCESSION   DBNGSH010001055 DBNGSH010000000
VERSION     DBNGSH010001055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4579503"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="AC16CY_23885"
     CDS             complement(<1..643)
                     /locus_tag="AC16CY_23885"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP family kinase ATP-binding protein"
                     /protein_id="HIR3356915.1"
                     /translation="MSLKTTEPALAGAAVGDYIDVPGSVANLGGGFDTLGVAVQLYLR
                     ARIVDVTDDGGTALSVVRSTPPVQGGNAVERAFGIAAQRTGRRAPTVTVEVTSEIPMA
                     AGLGSSAAATVAGLRLFERVTGTLDDGTLLGTAIEAEGHGDNAAPALFGGLNSVVDAV
                     RGEPLALRWSWPDDLRLVVVTPAVGLATAKARAALPREVARTDAIFNLQRVLAL"
     gene            complement(659..1927)
                     /locus_tag="AC16CY_23890"
     CDS             complement(659..1927)
                     /locus_tag="AC16CY_23890"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR3356916.1"
                     /translation="MPGAPLRVAILGFGTVGASVARILVERPELAAHVQLTHVFNRDV
                     SRKRADWVPSSVVWTENFDEVLATRPDVVVEVVGTVTPAREWVCQAIDRGASIVTANK
                     MLLATHGPELLQAASRARVRLRFEAAVGGGVPLIHGIREGLAGDVLTAVDGILNGTCN
                     YILTRMSATGEALDVVLRDAQRLGYAEADPSADVDGMDAAAKLVVLAGIAFHRHLRLD
                     DVPRRSIRPLTTVDFKYANRLGCSIRQVSRIQRVDDGFFGFVGPVLVPQGSSFGRNEG
                     ANNVVTIHGRYGGESSFSGAGAGGPATAVAVVSDLLTLSRNGQGERHSTEQWVPASFV
                     PPPPLPYFLRFVVRDQAGILATIAAALARRGINVDALVQEPGYPKDALPFVITVEPCE
                     EAALLEALKDLAAEDWHAQPPLAMPMLLGS"
     gene            1976..>2904
                     /locus_tag="AC16CY_23895"
     CDS             1976..>2904
                     /locus_tag="AC16CY_23895"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HIR3356917.1"
                     /translation="MEHHREPGARSPFTIGIIQDAALPDRAANLERTGRLVREAASRG
                     AQIVCLKELFQSPYFCKSQQIDRFDLAESIPGPTTDAMQTLARELAIVLVVPIFERQT
                     AGIYRNSAAVIDADGSLLGVYRKMHIPDDPLYHEKYYFTPGDAQAGEAGAERGFRVWR
                     TRYATIGVLICWDQWYPEAARITALLGAQVLFYPTAIGWHPAEKSEWGEAQVEAWRTI
                     QRAHAIANGIYVASPNRIGHEDEPGTNGITFFGHSFIADPFGRFVAEAGEGEQVLVAR
                     CDPALMETVRRNWPFLRDRRVDAYEPILNRYLGA"
BASE COUNT          536 a          988 c          941 g          439 t
ORIGIN      
        1 cgagcgccag cacgcgctgc agattgaaga tcgcgtccgt gcgcgccacc tcccggggca
       61 gcgcggcgcg tgccttggcc gtcgcaagcc cgacggccgg agtcacgacg acgagacgaa
      121 gatcgtcggg ccagctccag cgcagcgcga gcggttcccc gcgcaccgca tcgacgacgg
      181 aattgagacc gccaaagagc gccggcgcag cgttgtcgcc atgaccctca gcctcgatcg
      241 ccgtcccgag aagcgtgcca tcgtcgaggg tccctgtgac gcgctcgaac aacctcaagc
      301 ctgcgaccgt cgcggcagcg ctgctgccga gaccggcggc catcggaatc tcgctggtca
      361 cttcgaccgt gacggtcggc gctctcctgc cggtccgttg cgcggcgatc ccgaatgccc
      421 gctcgacggc gttacctccc tgcaccggcg gggtgcttcg aaccacgctg agcgccgttc
      481 ccccgtcgtc ggtcacatcc acaatccgtg cccgcaggta caactggacg gctacaccaa
      541 gggtatcgaa gccgccgccc aggttggcaa ccgagccggg aacgtcaatg taatccccga
      601 cagcggcgcc ggccagcgca ggctctgtcg tcttaagtga cacgaaataa aaacgatatc
      661 acgaacccag cagcatcggc atcgccagcg gcggctgagc atgccagtcc tctgccgcca
      721 gatccttcaa tgcttcgagc agggcggcct cttcgcaagg ctcgaccgtt atgacaaagg
      781 gcagtgcgtc cttgggatac ccgggctcct ggaccagggc atcgacattg attccccggc
      841 gcgcgagggc agcagcgatc gtggcaagaa tccccgcctg atcccgcacg acgaaccgga
      901 gaaagtacgg caggggcggg ggcgggacga acgaggcggg cacccactgt tccgtgctgt
      961 gcctctctcc ctgtccattg cgcgacagcg taagcagatc cgacacgacc gcgacggcgg
     1021 ttgccggacc accagcgccg gcgccactga agctgctctc accgccgtac cggccgtgga
     1081 tcgtcacgac gttgttcgcg ccctcgttgc ggccaaagct ggagccctgc ggaacgagca
     1141 ccggtcccac gaatccaaag aagccatcgt ccacccgctg gatccgggac acctgtcgaa
     1201 tgctgcatcc cagacggttc gcgtacttga aatcgacagt cgtcaaggga cgaatcgagc
     1261 ggcgaggcac gtcatccagc cggaggtggc gatgaaaggc gatgccggcg agcacgacga
     1321 gcttggccgc ggcgtccatg ccgtcaacat cggcgctcgg atcggcttcg gcataaccca
     1381 atcgctgcgc gtcccggagc acgacgtcca gcgcctcgcc ggtggcggac atccgcgtca
     1441 ggatgtagtt gcaggtgccg ttgaggatgc cgtctactgc cgtcagcaca tcgccggcca
     1501 gaccctcgcg aatcccgtga atgagcggca cgccgcctcc aacggctgcc tcgaatctca
     1561 gtcgcacgcg ggcgcgcgac gccgcctgaa ggagctcggg gccgtgcgtg gcaagcagca
     1621 tcttgttggc ggtgacgatc gacgctccgc gatcgatcgc ctggcagacc cactcgcgcg
     1681 cgggcgtcac cgtgccgacg acctccacga cgacatccgg acgggtcgcg agtacctcgt
     1741 cgaagttctc ggtccagacc accgacgacg gtacccagtc ggctcgcttg cgcgagacgt
     1801 cccgattgaa cacgtgggtc agctggacgt gagcggcaag ctcgggacgc tcgaccagga
     1861 ttcgggcgac cgacgcaccg acagtgccga acccgaggat ggcgacgcga agaggggctc
     1921 ccggcatgtc tcaacagtct acaaggtcca agctgccgta gactgaacaa cggacatgga
     1981 acatcaccga gagcccggag cccggagccc gttcacgatc ggcatcatcc aggatgccgc
     2041 gttgcccgac cgtgccgcga atctggagcg gaccgggcgg ctcgtacggg aagccgcctc
     2101 acgcggcgcc cagatcgtgt gcctgaagga gctgtttcag tctccgtatt tctgcaagtc
     2161 gcagcagatc gacaggttcg acctggccga atcgattccg ggtccgacca ccgacgccat
     2221 gcagacactc gcgcgggagc tggcgatcgt cctcgtcgtg ccgatcttcg agcggcagac
     2281 tgcgggcatc taccgcaact cggctgccgt catcgacgcg gacggttcct tgctcggggt
     2341 gtaccgaaag atgcacatcc cggacgaccc gctgtaccac gagaagtact acttcacgcc
     2401 gggcgacgca caagccggag aagcgggagc ggaacgcggc ttcagggtct ggcggacgcg
     2461 ctacgcgacg atcggcgtcc tgatctgctg ggatcagtgg tatcccgaag ccgcgcggat
     2521 cacggccctg ctcggcgccc aggtgttgtt ctacccgacc gccatcggat ggcaccccgc
     2581 cgagaagtcg gagtggggcg aggcgcaggt agaggcctgg cggacgatac aacgcgcgca
     2641 cgccatcgcg aacgggatct acgtggcctc tccgaatcgg atcggccacg aggacgagcc
     2701 ggggaccaac ggcatcacct tcttcgggca ctcgttcatc gcggatccct ttggccggtt
     2761 cgttgccgag gcgggcgagg gcgagcaggt gctggtcgcg cggtgcgatc cggcgctcat
     2821 ggagacggtc cgcaggaact ggccgttcct ccgcgacaga cgtgtggacg cgtacgaacc
     2881 gattctcaac cggtacctcg gcgc
//
LOCUS       DBNGSH010001056         1676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_4750411, whole genome shotgun sequence.
ACCESSION   DBNGSH010001056 DBNGSH010000000
VERSION     DBNGSH010001056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4750411"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1676
                     /locus_tag="AC16CY_23900"
     CDS             <1..>1676
                     /locus_tag="AC16CY_23900"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3356918.1"
                     /translation="NGLTFFIRQNALPDNRVLLRLAVKAGSIDEADDQRGLAHVLEHM
                     AFNGTAHFKPGELVSYLESIGAEFGAHVNAYTSFDETVYMLDVPTERPGVLERGFEAL
                     SDFAGGLTLDPAEIDRERGVVIEEWRGRLGAGTRMQEPQMKALFGASRYTDRMPIGTP
                     EILKSFTPERLRAFYRDFYRPDRMAVIVVGDIEPQAAEALVRKNFSGMPTPAPATRTV
                     YEIPPHAETRYVSVSDREAQGSSVTVMSKGPLHELRSMADYRRSLVQGLVFQMMNARF
                     AEIARQADAPFLAASAGSDTLGRTVEAASVSARVRDGAIARGLSALGEEVTRIRQHGF
                     GEAELDRARRTTLASFERAFNERDKAQSEPLASELIRHFLSGEASPGIAAEMELARRF
                     LSTISTTETAALAREMLGDGNRVVLATAPEKPGLAAIAEPALGEALRTGLAARVEPWR
                     DEIAGRALLASIPKPGSVSARREIPEIGVTVLTLSNGVEVWLKPTDFRNDQLLFTAYA
                     RGGLSRASADDYQNAALATSLVGMAGVGGITPTDLSKLLAGKIAGASPSLS"
BASE COUNT          256 a          570 c          606 g          244 t
ORIGIN      
        1 aacgggctga cgttcttcat ccgccagaac gcgcttcccg acaaccgtgt gctgctgcgc
       61 ctggccgtga aggccggttc gatcgacgaa gccgacgatc agcgcgggct ggcgcacgtg
      121 ctcgagcaca tggctttcaa cgggacggcg cacttcaagc ccggcgagct ggtgtcgtac
      181 ctcgagtcga tcggggccga attcggcgcg cacgtcaacg cctatacgag cttcgacgag
      241 accgtctaca tgctggacgt gccgaccgaa cgcccgggtg tgctcgagcg agggttcgag
      301 gcgctcagcg acttcgcggg cggcctcacc ctcgatcccg cggagatcga ccgcgaacgc
      361 ggcgtggtga tcgaggaatg gcgcggacgc ctgggcgcag ggacgcggat gcaggagccg
      421 cagatgaagg cgctcttcgg cgcgtcgcgc tacaccgacc gcatgccgat cggcacgccg
      481 gagatcctca agagcttcac gcccgagcgt ctcagggcgt tctatcgcga cttctaccgc
      541 cccgacagga tggcggtgat cgtggtcggc gacatcgagc cccaggcggc cgaagcgctc
      601 gtgcgaaaga acttctcggg gatgccgaca cccgcgccgg ccacgcggac ggtgtacgag
      661 attccgccgc acgccgaaac gcgctacgtg tcggtgtcgg accgcgaggc ccagggctcg
      721 tcggtcacgg tgatgagcaa gggtccgctt cacgagctgc ggtcgatggc cgactaccgt
      781 cgctcgctcg tccaggggct cgtcttccag atgatgaacg cgaggttcgc cgagatcgcg
      841 cggcaggcgg acgcgccgtt tctcgcggcg tcggcgggca gcgacacgct cgggcggacc
      901 gtcgaggcgg cctccgtctc ggcccgcgtg cgggacggcg ccatcgcgcg cggcctgagc
      961 gcgctcggcg aggaagtgac gcgcatccgg cagcacggct tcggcgaagc cgagctggac
     1021 cgggccaggc gcacgacgct cgccagcttc gagcgcgcct tcaacgagcg ggacaaggcg
     1081 cagagcgagc cgttggcctc agagctcatt cggcatttcc tgagcgggga ggcctcgccc
     1141 gggatcgccg ccgaaatgga gctggcgcgg cgcttcctct cgaccatctc caccacggag
     1201 acggccgcgc tcgcgcgcga gatgctgggc gacggcaacc gcgtcgtgct ggcgacggcg
     1261 ccggagaagc cggggctcgc cgcgatcgcc gagcccgcgc ttggcgaggc gcttcgaacg
     1321 gggctggcag cccgcgtgga gccgtggcgc gacgagatcg cggggcgggc gctgctcgcc
     1381 agcatcccga aaccgggttc ggtgtccgcg cgtcgcgaga ttcccgaaat cggcgtgacg
     1441 gtgctgactc tgtccaacgg cgtcgaggtg tggctgaagc cgaccgactt ccgcaacgac
     1501 cagctgctct tcacggccta cgcccgcggc ggcttgtcgc gtgcgtccgc ggatgactat
     1561 cagaacgcgg cgctcgccac ctcgctcgtg ggcatggccg gcgtgggcgg catcacgccc
     1621 accgacctgt cgaagctgct ggccgggaag attgcggggg cgtcgccgtc cctgtc
//
LOCUS       DBNGSH010001057         5580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_165289, whole genome shotgun sequence.
ACCESSION   DBNGSH010001057 DBNGSH010000000
VERSION     DBNGSH010001057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_165289"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="AC16CY_23905"
     CDS             complement(<1..1372)
                     /locus_tag="AC16CY_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3356919.1"
                     /translation="MIGVAADPADLEVVREFFELFKTPWEVARPNRKYRSVLCTDGNA
                     GGFDADVIFAFGADPHPADLQADMLPSPIEDAVVRFDDAEIPIYGRASVFAAAPPARL
                     TAHGRPLEYRRRRGTAMVHRVGYDLFREVRHLLTVGQPPSQALIPTLDRHIALLRRLL
                     TEAGVPFLEIPPRPARHDFICCLTHDIDFYGIRRHGLDLTMAGFLLRASIGTVVDLAR
                     SRRTLRDAVRNFAAVCSLPLVYAGLMRDFWRPFDDYAAVEDPKRSTFFLVPFKNRPGQ
                     SPAGLTDATRAVAYQASDIAGDVAAAAARGSEIGLHGIDAWRDPGAGRAEMREIASIT
                     GRPKTGVRMHWLYFSEQAPRQLEAAAFDYDSTWGYNDAVGFRAGTSQVFAFPGVPTLL
                     ELPLSIMDTALFAWDRWAMTRADARRHCDVVLAHAAQTGGTVVVNWHCRSLAPERLWH
                     SFYRELL"
     gene            complement(1386..2408)
                     /locus_tag="AC16CY_23910"
     CDS             complement(1386..2408)
                     /locus_tag="AC16CY_23910"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR3356920.1"
                     /translation="MLHELDPLRDDRWPAFVERHPRSSLFHTRAWLEALRRSYGYEPV
                     VYTDAAPSEALRNALVFCRVSSWITGRRLVSLPFSDHCEPLVEDAASGRVLLEALRSR
                     IGQEGRYIEVRPLLTPPATDGYVPSAHFVHHHIDIRPSLDEIFGTFHRSHAQRGIRRA
                     ERLGVSCVVGRSKELVGDFYRLHAMTRGRHRMPVQPAIWFRSLVECFDAQASVYVARR
                     GTTPIAALFTLDHKGTRVYKYGCSDVAFNRYAGTLALMWRAIQDARQNGQLQFDLGRS
                     DADDAGLIAFKDHLGARRSSLTYYRYSRTSSAPGWTPRLSRGVGAMLPSPFRAFLGRA
                     AYRHFG"
     gene            complement(2418..3299)
                     /locus_tag="AC16CY_23915"
     CDS             complement(2418..3299)
                     /locus_tag="AC16CY_23915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HIR3356921.1"
                     /translation="MSRMRLPARTIVLAAFTLCLAAINLRPLVQLVATTQSDSSASHV
                     LAVPLVSLALIVLNRKDIFSTVTTSNAAGLAVMLGGAGLSLAGFARHGASALSVQVAG
                     LVVCWIAGYMLIYGVAAARRAAFPLAFLLFTIPPPPVVIDVATQVLKAGSSEIVAGLF
                     SLVGTPYYREGDVFALPGVTIEIADECSGIRSSTALLLTSLLAGHMFLSRGWSRAALV
                     MVVLPVALLKNGIRIVSLSLLSLHVDPSFLTGQLHHEGGIVFFLLALGLMMPFLFLLR
                     RSEERSADVPVGYVTQV"
     gene            complement(3296..4735)
                     /locus_tag="AC16CY_23920"
     CDS             complement(3296..4735)
                     /locus_tag="AC16CY_23920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3356922.1"
                     /translation="MLLHIPSRALPEPNPFQLVNEPAGSASVEGKRMHQCLFDYFGCP
                     RDLVALSAAERLLSDPGYFAFGDAVAYGRSDRCRVTSLPTDGLEDSLPAVAATHDGVR
                     LPFDLDEVVTNLRQERYRQPARGYLEQATSVGTIQRLYYLLRPVLPVAVRRHLQRVRL
                     SGWERIAFPRWPLETAVDSLMRTTLGLLVRQNGGRAIPFIWFWPEGAPGCVVMTHDVE
                     GAAGGEFCGQLMDLDDAHDIRSAFQVVPEMPGQPTRALVNDMRKRGFEVNLHDLNHDG
                     YLFHERAEFLKRAALINRYTRELECRGFRSGAMYREQDWFDAFEFSYDMSVPNVAHLE
                     PQRGGCCTVMPYFVGNVLELPLTTTQDYSLFHIIGDYSSALWKQQVELILAFNGFVSF
                     IAHPDYLIEKRARAVYVSLLEHLKMLRETRHVWMALPADVDRWWRNRRRMTLVERGGA
                     WRIEGPDAERARLAYAELDGERVVYRLAA"
     gene            complement(4739..>5580)
                     /locus_tag="AC16CY_23925"
     CDS             complement(4739..>5580)
                     /locus_tag="AC16CY_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3356923.1"
                     /translation="PTEILTSPDVDAVAVVTPVWTHYELAKAALEHGKHVFVEKPFTS
                     TSRQAEELIELADRKNLKIMVDHTFLFSGAVRKIREIVEKGTLGSLYYFDSTRVNLGL
                     FQHDVSVVWDLAPHDLSIMDHILHTKPEAVVTTGAKHINDLADMAFITVFFPGNLIAH
                     INVNWLSPVKVRMTLIGGKDKMLVWNDLEPDEKIRVYDKGVDVTNGQGVHDLLVSYRS
                     GDVYSPRVEGTEALKYELNYFVDCIVKGETPVNDGAAGLRVVRLLEAAERSLNERGRI
                     IPL"
BASE COUNT         1070 a         1828 c         1810 g          872 t
ORIGIN      
        1 cgagcagctc gcgatagaac gagtgccaga ggcgttcggg cgcgaggctg cggcagtgcc
       61 aattgacgac cacggtgccg ccggtctgag ccgcgtgagc cagcaccacg tcacagtgcc
      121 gccgtgcatc ggcgcgcgtc atcgcccaac gatcccacgc aaacagcgcc gtgtccatga
      181 tcgacagcgg cagctcgagc agcgtcggca cacccgggaa cgcgaagacc tgtgacgtcc
      241 cggcccgaaa ccccacggca tcgttgtagc cccacgtcga gtcgtagtcg aacgcggccg
      301 cctccagctg tcgcggggcc tgttccgaga agtacaacca gtgcatcctg acgcccgtct
      361 tcgggcggcc tgtgatcgac gcgatctccc gcatttcggc ccgccctgca ccgggatccc
      421 gccacgcatc gataccgtga aggccgatct cgctcccgcg agcggccgca gcggcgacgt
      481 ccccggcgat atcgctcgcc tggtaggcca cggcgcgtgt ggcatccgtc aggcccgcgg
      541 gggactggcc ggggcggttc ttgaatggca cgagaaagaa cgtcgagcgc ttcgggtctt
      601 ccaccgccgc gtagtcgtcg aacggccgcc agaagtcccg catcaggccg gcgtacacga
      661 gcggcagcga acacacggcg gcgaagtttc ggacggcgtc gcggagggtt cgacgagacc
      721 gtgcaagatc gacaacggtt ccgatcgagg cgcgcaggag gaaccccgcc atcgtgaggt
      781 ccaggccgtg gcggcggata ccgtagaaat cgatatcgtg cgtgaggcag cagatgaagt
      841 cgtgccgagc cggccgcggg gggatttcca gaaacggcac gccggcctcc gtcagcaggc
      901 gacggagcag tgctatgtgc cggtcgagcg tcgggatcag cgcctgcgag ggcggctggc
      961 cgaccgtgag cagatgccgg acttccctga agaggtcgta gccgacccgg tgcaccatcg
     1021 ccgtgccgcg gcggcgccgg tactcgagcg gccggccgtg cgccgtgagc cgtgccggcg
     1081 gcgcggcggc aaagaccgat gcccgcccgt agatcgggat ctccgcgtca tcgaatcgaa
     1141 cgaccgcatc ctcgatgggg ctgggaagca tgtcagcctg gaggtccgca ggatgcggat
     1201 ccgcgccgaa cgcgaagatg acgtcagcgt cgaacccgcc ggcgttgccg tctgtacaca
     1261 agaccgatcg atacttccgg ttcggcctcg caacctccca cggggtcttg aacaactcga
     1321 agaactcccg cacgacctcg aggtcggccg ggtcggcagc tacgccaatc accgcagaaa
     1381 cgatttcagc caaagtgacg gtacgcggcc ctcccgagga aggcgcggaa aggcgatggg
     1441 agcatggcgc ccactcctct ggagagccgg ggcgtccagc cgggggcgga cgacgttcga
     1501 gagtagcgat agtacgtcag gctcgaccgc cgggccccga ggtgatcctt gaacgcaatg
     1561 agtccggcat cgtcggcgtc ggaccgcccc aggtcgaatt gcagctgtcc gttctgcctg
     1621 gcgtcctgga tcgccctcca catcagcgcg agcgtgcctg catagcggtt gaaggccacg
     1681 tccgagcatc cgtacttgta gacgcgagtc cccttgtgat cgagcgtaaa cagggcggcg
     1741 atcggcgtcg tcccgcgccg tgcgacatag acgctcgcct gcgcgtcgaa gcactcgacg
     1801 aggctccgga accagatcgc cggctgaacc ggcatccggt gacgcccgcg ggtcatcgca
     1861 tgcaaccgat agaaatcgcc gaccagctct ttcgatcgcc cgacgacgca cgagacgccg
     1921 agccgctcgg cgcgccggat gccccgctgc gcgtgactcc tgtgaaacgt cccgaagatc
     1981 tcatcgagcg acggccggat gtcgatgtga tgatgcacga aatgcgctga gggcacatac
     2041 ccgtccgtcg ccgggggcgt caggagggga cgcacctcga tgtatcggcc ttcctgcccg
     2101 atgcgcgatc tcaacgcttc gagcaacacg cgaccactgg cagcgtcctc gaccaggggc
     2161 tcacagtggt ccgagaacgg cagagagacg agccgacggc ccgtgatcca gctgctgaca
     2221 cggcagaaca cgagcgcgtt gcgcagtgcc tcagacggcg cggcgtccgt gtagacgacc
     2281 ggctcgtagc catacgaacg acgcagcgct tccagccagg ccctcgtgtg gaacaaggaa
     2341 ctgcgcggat gccgctcgac gaatgcgggc catcggtcat cgcggagcgg gtcgagctca
     2401 tgcagcaagg cggccgttca cacctgtgtc acgtagccaa ccggaacatc ggcagatcgt
     2461 tcttccgacc gacgaagcag aaacaggaac ggcatcatca agccgagcgc caacagaaag
     2521 aacacgattc cgccttcgtg atggagctgc cccgtgagga acgacggatc gacgtgcagg
     2581 gacagaagcg acaggctcac gatccggatg ccgttcttga gcagcgccac cggaagaacc
     2641 accatcacca gcgctgcccg ggaccaccct cgactcagga acatatggcc agccagcagg
     2701 ctcgtcagga gaagggcggt gctcgatcgt atcccgctgc attcgtcggc gatttcgatc
     2761 gtcactccgg gaagagcaaa tacgtcacct tccctgtaat acggcgtccc gacgaggctg
     2821 aagagccctg cgacgatttc cgacgagccg gccttcagga cctgggtcgc cacgtcgatg
     2881 accaccgggg ggggtgggat cgtgaacaac aggaaggcca gcggaaatgc cgccctgcga
     2941 gcagccgcga cgccgtagat cagcatgtat ccggcgatcc agcacacgac cagcccggcc
     3001 acctgcaccg acagtgcact cgctccgtga cgggcgaacc cggcgagcga caggcccgcg
     3061 ccgcccagca tcaccgcgag accggccgcg ttcgaggtcg tcaccgtcga gaaaatgtct
     3121 ttccggttga ggacgatgag cgccagggac accagcggaa cggcgaggac atgggatgcc
     3181 gacgaatccg actgcgtcgt cgccaccagc tgaaccagcg gccgcaggtt gattgccgcc
     3241 aggcagagcg taaaggcggc aaggacgatc gtgcgggccg gcaggcgcat tcgactcatg
     3301 cggccagtct gtagacgact ctctcgccgt ccagctcggc ataggcaagc cgcgcccgct
     3361 cggcgtcggg cccctcgatc cgccaggcac cgccccgctc gacgagcgtc atccgtcttc
     3421 ggtttcgcca ccagcgatcg acgtcggcgg gcagcgccat ccagacatgc cgcgtctcgc
     3481 gaagcatctt caggtgctcg agcagactca cgtagacggc gcgcgcgcgc ttttcgatca
     3541 ggtagtcggg atgcgcgatg aagctgacaa agccgttgaa cgccaggatc agctcgacct
     3601 gctgcttcca caacgcgctc gagtagtcgc cgatgatgtg aaagagcgag tagtcctgcg
     3661 tcgtcgtcag gggcagctcg aggacgttgc cgacgaaata tggcatcacg gtgcagcagc
     3721 cgccgcgctg cggctcgagg tgcgcgacgt tcgggacgga catgtcgtac gagaactcga
     3781 aggcgtcgaa ccagtcctgc tcgcgataca tcgcgcccga gcggaaaccg cggcactcga
     3841 gctcccgtgt ataccggttg atcagggcgg ctcgcttgag gaattccgcg cgctcgtgga
     3901 acaggtagcc gtcatggttc aggtcgtgca ggttcacctc gaacccgcgc ttgcgcatgt
     3961 cgttcacgag cgctctcgtc ggctggccgg gcatctcggg aacaacctga aacgccgacc
     4021 ggatgtcgtg ggcgtcgtcg agatccatga gttggccgca gaactcaccc ccggcggcgc
     4081 cttccacgtc gtgcgtcatc acgacgcaac cgggagcgcc ctcgggccag aaccagatga
     4141 agggaatggc tcgtccaccg ttctgacgga ccagcaagcc gagcgtcgtt cgcatcagcg
     4201 aatcaacggc agtctcgagc ggccaccgcg gaaaggcaat ccgttcccag ccgctcaacc
     4261 gcacgcgctg caagtgccgc ctgacagcca ctggcagcac gggccgcagg aggtaataga
     4321 gcctctgaat cgtcccgaca ctcgtcgcct gttccaggta gccgcgcgca ggctggcggt
     4381 agcgctcctg gcgcaggttg gtgacgactt catccagatc gaacggaagt cgcacaccgt
     4441 catgggtggc ggcgacggcc gggagcgagt cttcgagacc gtcggtgggc agcgaagtga
     4501 cccggcaccg atcggaccgt ccgtacgcca cggcgtcccc gaacgcgaaa tagcccgggt
     4561 cggacagaag acgttcagca gccgagagtg caaccaggtc tcgtggacaa ccgaagtagt
     4621 cgaacagaca ctggtgcatc cgcttacctt cgaccgatgc cgacccagca ggctcgttga
     4681 ccagttgaaa cgggttcggc tccggcagcg cgcgcgatgg gatgtggagc agcaagactt
     4741 acagcgggat gattcgtccc cgttcgttca gcgaccgttc ggcggcctcg agcaaccgca
     4801 ccacccggag gcctgcagca ccatcgttca ccggtgtctc gcccttgacg atgcagtcca
     4861 cgaagtagtt gagctcgtat ttgagcgcct ccgtcccctc cacgcgaggg gagtacacgt
     4921 caccggagcg gtagctgacg agcaggtcgt ggacgccctg gccgttcgtg acgtcgactc
     4981 ccttgtcgta caccctgatc ttctcgtcgg gctcgaggtc gttccacacc agcatcttgt
     5041 ccttgccgcc gatgagcgtc atgcgcactt tgaccgggga cagccagttc acgttgatat
     5101 gcgcgatgag attcccgggg aagaacacgg tgatgaaggc catgtccgcc aggtcgttga
     5161 tgtgtttggc gcccgtggtg acgaccgcct cgggcttggt gtggaggatg tggtccatga
     5221 tcgacagatc atgaggcgcc aggtcccata ccacgctcac gtcgtgctgg aagaggccca
     5281 ggttgacccg tgtcgaatcg aagtagtaca gcgaccccag cgtccccttt tcgacgatct
     5341 cgcgaatctt cctgacggcg ccgctgaaca ggaaagtgtg gtccaccatg atcttcaggt
     5401 tcttcctgtc ggcgagctcg atgagctcct cggcctgtcg agacgtggac gtgaacggct
     5461 tctccacgaa cacgtgcttg ccgtgctcga gcgccgcctt ggccagctcg taatgcgtcc
     5521 acaccggcgt gacaacggcg acggcgtcga catccggcga cgtcagaatc tccgtcgggt
//
LOCUS       DBNGSH010001058         6454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3899476, whole genome shotgun sequence.
ACCESSION   DBNGSH010001058 DBNGSH010000000
VERSION     DBNGSH010001058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3899476"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="AC16CY_23930"
     CDS             complement(<1..863)
                     /locus_tag="AC16CY_23930"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3356924.1"
                     /translation="MALAPELQSLVQAAAQAAEAGDFVSAERLLREVASRQESELGPR
                     HPDLANTFNNLGVLCEKAGTPDEAERCYRRAYAIASSALAPDDPFVVTSRNNLREFCE
                     ARGRPFELEAPSPVELEAPEVPSPVELKVPSPVELEAPAPVADDEPLAVLPLPIESRD
                     GGGRGGRAFAVMLGGLGLVLLAWYGPWRDSAEPPVGADTSSIAPPAAAPAPTEDAGPP
                     PADVPSTAAPRTTAPAPAPVPVKSSRALESPARTASAPAAIAVVEARLCRSLSRSDWR
                     CVPAESEGGPGP"
     gene            962..3313
                     /locus_tag="AC16CY_23935"
     CDS             962..3313
                     /locus_tag="AC16CY_23935"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3356925.1"
                     /translation="MRADDLLELFFAQSLDGFFFMMLDEPVRWDDSVDKEKTLDYVFD
                     HQRVTKVNDAMLAQYGATREAFLGTTPKGLFGHDLDHGRRVWRDFFDRGRLHIETDER
                     RVDGTPIRIEGDYLCFYDEQGRVTGHFGIQRDVTERERANQALRQYNRRLQVLQDIHL
                     DILGSQSPRQIARATIRHIRDLIPCSRVSVAALDQARGTGTLIALHQEGPTNLHEGAV
                     VPIGSFGSVDDLRAGAVRSVASLAPGTGVLETLRSEGLRSFINVPLLADGNLIGVLNV
                     SSVEEGAFGPDAIEIAREVASTLALALWQAQLRERIERYANELEDRVAVRTADLERSE
                     NRLAAILNALPDLVFVADGDGRYLEILTSREELLYLAAAEMKGRRFHDILPEAVADTH
                     LRRVRETLATRTTQTFEYSLRVRAGERWFEGRTGLLGVQIDHRPAVAVIARDITDRKR
                     AEELESQNIYLQEELKVERSFGEIVGQSPAMQRVFKAIELVAETDSTVLLLGETGTGK
                     ELVARAIHKLSRRQHAVMVKVNCGALPSSLAESELFGHERGAFTGAVQQKKGRFELAN
                     GGTIFLDEVGELSADVQVKLLRVLQEQEIERLGSARPTKVNVRVVAATNRDLPVEAQR
                     GTFRSDLFYRLNVFPIHLPPLRERRQDVPLLAGHFVTEFARRIGKPVERIGPQVIGRL
                     SSYDWPGNIRELANVLERAVILCDGPVILEEHLGALSREPPGTDKDSFVTLEEMERRH
                     ILRALAQTGGVLAGPQGAARLLGMSRSTVWSRMRKLGIQPPRD"
     gene            3533..3970
                     /locus_tag="AC16CY_23940"
     CDS             3533..3970
                     /locus_tag="AC16CY_23940"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3356926.1"
                     /translation="MFTRRQAVRWAELWLACWNDGDFDTLLAVYRDDASFDSPSTAAE
                     EGSPVDRRDAFKRYWAAIPYGIHASPAELDRVSFDPETRALTIVYTADLGGARLRGCD
                     LLTLDEDSRIITGEPCVGRLDPRPASDGAFAHESSVETAGARR"
     gene            3991..6444
                     /locus_tag="AC16CY_23945"
     CDS             3991..6444
                     /locus_tag="AC16CY_23945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3356927.1"
                     /translation="MIGIVIGVWGLLAGPAAAIAETAALAPELTGLGTLHVPVTTGVP
                     RAQRFFDQGMRLLYAFNHAEAIRAFREAARLDPRLAMAHWGHALALGPNLNAPMTEEN
                     RRQAYAAIGRAQRLASSATPRERALVDALAARYAPDGKGNRAVLDRAYAAAMRKAAER
                     FPDDPDVLTLYADAQMNTMPWDYWTKGGLAKPETQWTLDVLERVITRQPDHVGALHYH
                     IHLLEASATPQRAEASADRLGSLMPSAGHMVHMPAHIYIRVGRYADAAEANVRAIAAD
                     EDYLAQCQVQGLYPISYYPHNLHFLWAAATLEGRRAVALDAARKVAAKVPHHHAGALS
                     WTADFPVTPMLAYARFGLWQDALTEPKPPANEPYAVGIWHYSRGLAFAARAQLDRAVG
                     ELAALKAITKHEAFATTLKDLPLLTNLQIAARIVDGELTMRRGDAASAIALLKEAVEI
                     EDGIPYNEPPVWHHPSRQVLGALLLEAGRAAEAEAVYREDLARFPENGWSLFGLKQSL
                     DAQGRGAEGADVQRRFEKAWARADIRLTWSRIAGASKDAHPRERFATLPDGTRLAYVE
                     QGDPNGTPVILLHGYTDSRRSFDRILPHLSGSLRVFAVTQRGHGTSDKPAGRYESDVF
                     ARDVAGFLDVFGIERAVIVGHSMGSTVAQRFAVEYPHRTQALVLEAAFMPRPAGAELT
                     AFLGEVSGLKDPIDPAFVRGFQQSTLTRPVPPEFFDTIVGESMKVPARVWKAALEPYR
                     TTNFAARLAEIRVPTLLVWGEHDAFTLRAEQEALTQAIPGATLVVYSGTGHCPHWEEP
                     ERFAGEVASFIAHVVSIEG"
BASE COUNT         1075 a         2140 c         2212 g         1027 t
ORIGIN      
        1 gggccggggc cgccttcact ctcggccggc acgcaacgcc agtccgatct ggacaagctt
       61 cggcaaagcc gcgcctcgac gacagcaatc gccgccggcg cggacgccgt tcgagccggc
      121 gactccaggg cgcgtgacga ctttaccggg actggcgcag gtgcaggcgc ggtcgtgcgc
      181 ggcgccgccg tcgagggcac gtctgctgga ggcggccctg cgtcctctgt cggtgccggt
      241 gcagcggctg gcggagcgat tgacgaggtg tcggcgccta ccgggggctc ggccgaatca
      301 cgccacggcc cgtaccaggc aagcaggacg aggccgagac cgccgagcat cacagcaaag
      361 gcacggcctc cgcggccgcc accgtcccgc gactcgatcg gaaggggaag caccgccagc
      421 ggttcgtcgt ctgctacggg ggcgggcgct tccagttcga caggggaggg cactttcagt
      481 tcaacggggg agggcacttc gggcgcttcc agttcgacgg gcgagggcgc ttccagttca
      541 aacggacgtc cgcgagcctc gcagaactca cgaaggttgt tccggctcgt gacgacgaac
      601 ggatcgtccg gcgcgagagc ggaggacgca atcgcgtacg cgcgtcgata gcagcgctcc
      661 gcctcgtccg gcgttccggc cttttcgcag agaacgccga gattgttgaa cgtgttggcg
      721 aggtcgggat gccggggacc gagttcggat tcctggcggc tggcgacctc gcgcagcaac
      781 cgctcggccg agacaaagtc tcccgcttcg gccgcctgtg cggccgcctg gacaagcgac
      841 tgaagctcgg gagcgagggc catgtacttg ctccggctat cgccccattg tagaccgcgc
      901 cgcagggctt cgtggcgccc ggaccccgcg cgcgttagga tccgccattg acgagctcag
      961 catgagagcc gacgacctcc tcgagctgtt ctttgcccag tccctggacg gcttcttctt
     1021 catgatgctc gacgagcccg tccgttggga cgactccgtc gacaaggaga agacgctcga
     1081 ttacgtcttc gatcaccaga gggtgaccaa ggtgaacgac gcgatgctgg cgcagtacgg
     1141 cgccacgcgc gaggcgttcc tggggaccac gccgaagggc ctgttcggcc acgacctcga
     1201 ccacgggcgg cgcgtctggc gcgacttctt cgatcgcggc cggctccaca tcgagaccga
     1261 cgaacgccgg gtcgacggaa cgccaatccg aatcgaaggg gactacctct gcttctacga
     1321 cgagcagggg cgcgtcaccg gccacttcgg tattcagcgc gacgtgacag aacgcgagcg
     1381 cgcgaaccag gcgctgcgtc agtacaaccg gcggctgcag gtgctgcagg acatccacct
     1441 cgacatcctc ggctcgcagt cgccccggca gatcgcgcgg gccacgattc gccacatccg
     1501 cgacctgatc ccgtgcagcc gggtgagcgt ggccgccctc gatcaggccc gcggcacggg
     1561 gacgctcatc gcgctccacc aggagggtcc cacgaatctg cacgagggcg ccgtcgtgcc
     1621 gatcgggtcc ttcggcagcg ttgacgatct tcgggcgggc gccgtgcgaa gcgtcgccag
     1681 cctggcgccg gggaccgggg tgctggagac gctcaggagc gagggcctgc gatcgttcat
     1741 caacgtgccg ctgctcgcgg acggcaatct catcggcgtg ctcaacgtct cgtcggtcga
     1801 ggagggggcg ttcggccctg atgcgatcga gatcgcccgt gaagtggcga gcacgctggc
     1861 ccttgccctg tggcaggccc agttgcggga acgcatcgag cgctatgcaa acgagctgga
     1921 ggaccgcgtc gccgtgagga ccgcggacct cgagcggagc gaaaaccggc tggcggcgat
     1981 cctcaacgcg cttccggatc tcgtgttcgt cgccgacggc gacggccggt atctcgagat
     2041 cctgacgtcc agggaagagc tgctctacct cgcagccgct gagatgaagg gaaggcggtt
     2101 ccacgacatc ctgccggagg cggtcgccga tactcacctc cggcgggtac gcgagacgct
     2161 cgcgacgcgc acgacccaga cgttcgagta ctcccttcgc gtgcgggcag gagagcgatg
     2221 gttcgaaggc cgcacgggcc tgctcggcgt gcagatcgat catcgcccgg cggtggccgt
     2281 catcgcgcgc gacatcaccg atcgcaagcg ggccgaggag ctggagtcgc agaacatcta
     2341 cctccaggag gagctgaagg tcgaaagaag cttcggcgag atcgtcggcc aatcgcccgc
     2401 catgcagcgc gtgttcaagg cgatcgagtt ggtggccgag accgattcca ccgtgctgct
     2461 gctgggcgag accggcaccg gcaaggagct cgtcgcgcgg gcgattcaca agctgagccg
     2521 gcgtcagcac gccgtcatgg tgaaggtgaa ctgtggtgcg ctcccctcga gcctggccga
     2581 gagcgagttg ttcgggcacg aacggggcgc cttcacgggg gccgtgcagc agaaaaaggg
     2641 acgcttcgag ctcgccaacg gcggcacgat attcctggac gaagtggggg agttgtccgc
     2701 cgacgtgcag gtgaagctgc tgcgcgtcct ccaggagcag gagatcgagc gcctcggctc
     2761 agcccgcccg acaaaggtga acgtgcgcgt cgtcgccgcg accaaccgtg acctgcccgt
     2821 ggaagcgcag cgcggcacgt tcagatccga cctgttctat cgcctcaacg tctttcccat
     2881 tcatctgccg ccgctcagag agcgccggca ggacgtcccg ctgctcgccg gtcacttcgt
     2941 gaccgagttc gcccggcgca tcggaaagcc cgtcgagcgt atcggtcccc aggtcatcgg
     3001 gcgcctcagc tcgtacgact ggccagggaa tatccgcgag cttgccaacg tcctcgagcg
     3061 cgcggtcatc ctctgcgacg gtccggtgat tctcgaagag cacctcggcg ctctcagccg
     3121 tgaaccccct ggaaccgaca aagactcgtt cgtcacgctc gaagagatgg agcggcggca
     3181 catcctgcgc gcgttggccc agaccggtgg tgtgctggcc ggaccgcagg gcgccgcgcg
     3241 cctgctcggc atgagccgat cgaccgtctg gtcccgaatg cggaagctcg ggattcaacc
     3301 ccctcgcgac taagcctcca cgcgacagat tgatgctgag atatcggtac gtctgccgat
     3361 atctcagcac gacatccggt ccacggacgc cagcgagact cgtcaggcgc cacgcaagtc
     3421 attgaaggca gtcgatatgt gcgattgcca tcctcacaat ggccaggctg gcacattgat
     3481 cgctcttgtc gatggacgcc gcaagtcatg gcatcgcggc gagggaggtc acatgtttac
     3541 ccgcaggcag gccgtccggt gggccgaact ctggctcgcg tgctggaacg acggcgactt
     3601 cgacacgctg ctcgctgtct accgggacga cgccagtttc gacagtccgt caactgccgc
     3661 ggaggaaggc tcgccggtcg atcgtcgaga cgcgttcaag cgctactggg ccgccatccc
     3721 gtacggcatc catgcctcgc cggccgaact cgaccgcgtg tctttcgacc cggaaacacg
     3781 agcgctgacg atcgtctata ccgccgacct cggcggagcc cggctgcgcg gctgcgatct
     3841 cctgacgctc gatgaggaca gtcgaatcat cacaggcgag ccctgtgtcg gtcgtctgga
     3901 tccgcgtccc gcgagcgacg gggcgtttgc tcacgaatcg tccgttgaaa ctgcaggagc
     3961 acgacgatga gagccacggt cagaggaccc atgattggaa tcgtcatcgg cgtttggggg
     4021 ctgttggcgg ggccggccgc ggccatcgcc gagacggcgg cgcttgcccc ggagctgacc
     4081 ggcctcggca cccttcacgt cccggtcacg accggcgtac cgcgcgcgca gcgctttttc
     4141 gatcagggca tgcggttgtt gtatgcgttc aaccacgccg aggccattcg tgcgttccgc
     4201 gaggcggcgc gtttggatcc caggctggcc atggcgcact gggggcatgc gcttgccctc
     4261 ggtccgaatc tgaatgcgcc catgactgaa gagaaccgcc gacaggcgta cgccgcgatc
     4321 ggacgtgcgc agcggctcgc gtcatccgcg actccgcgcg agcgagccct cgtggatgcg
     4381 cttgccgcgc gctacgcgcc ggacggaaag ggcaatcggg cggtgctcga ccgcgcgtat
     4441 gcggccgcga tgcggaaagc ggcggagcgt tttcccgacg atccggacgt cctgacgctc
     4501 tatgcggacg cgcagatgaa cacgatgccg tgggattact ggacgaaggg tggcctggcc
     4561 aagccggaaa cgcagtggac gctcgatgtg ctcgagcgcg tcatcacccg gcagccggac
     4621 cacgtgggcg cgctccacta tcacatccac ctgctcgagg cgtcggcgac cccgcaacgc
     4681 gccgaggcga gtgccgatcg gctcggatcg ctcatgccgt cggcggggca catggtccac
     4741 atgcccgcgc acatctacat tcgcgtaggc cgttatgcgg acgccgccga ggccaatgta
     4801 cgcgccatcg cggccgacga agactacctc gcgcagtgcc aggtccaggg gctctatccg
     4861 atcagctact accctcacaa ccttcacttc ctgtgggcgg cggcgacgct cgaggggcgg
     4921 cgggcggtcg ccctggacgc cgcgcgcaag gtggcggcga aggttccgca tcaccacgcc
     4981 ggcgcgcttt cgtggacggc cgactttccc gtgacgccca tgctggctta cgcacgattc
     5041 ggactgtggc aggacgcgct gaccgagccg aagccacccg ctaacgagcc gtacgccgtc
     5101 ggtatctggc actattcgcg agggctggcg tttgcggccc gcgctcagct cgatcgcgcc
     5161 gtcggcgagc tggcggcgct gaaagcgatc acgaagcacg aggccttcgc gacaacgctg
     5221 aaggacctgc cccttctcac caacctgcag atcgcggcgc gcatcgtcga cggcgagctg
     5281 accatgcgcc gcggagacgc cgcgtcggcc atcgcgctgc tgaaggaggc cgttgagatc
     5341 gaagacggga ttccgtacaa cgagcccccg gtctggcacc atccgtcgcg tcaggtgctt
     5401 ggcgcgctgc tgctcgaggc cgggcgcgcg gctgaggccg aggccgtgta ccgggaggat
     5461 ctggcccgct tccccgagaa cggatggtcg ttgttcgggc tgaaacagag tctcgacgca
     5521 cagggtcgcg gcgccgaggg cgccgacgtg cagcggcggt tcgagaaggc gtgggcccgc
     5581 gcggacatcc gattgacctg gtcgcgaatc gcaggcgcat ccaaggacgc gcatccgcgg
     5641 gagcggttcg ccacgctgcc ggacgggacg cggctggcgt acgtcgagca aggcgatccg
     5701 aacggcacgc ccgtgatcct gctgcacggg tacacagact cacgccgctc gttcgatcgc
     5761 attcttccgc atctctcggg gtcgcttcgc gtgtttgccg tcacgcagcg cgggcacgga
     5821 acgtccgaca agccggcagg ccgctacgag tccgacgtgt tcgcacgcga cgtcgcaggc
     5881 tttctcgacg tcttcggcat cgagcgcgcc gtgatcgtgg ggcactcgat gggatcgacc
     5941 gtggcgcagc gcttcgccgt cgagtacccg catcgcacgc aggcgctcgt cctcgaggcg
     6001 gcgttcatgc ctcgcccggc cggcgcggag ctcaccgcgt ttctggggga ggtgtcgggg
     6061 ctgaaggatc caatcgaccc ggcgttcgtc cgcggctttc agcagagcac gctgacacgg
     6121 ccggtacccc cggagttctt cgacacgatc gtgggcgagt cgatgaaggt tccggcgcgg
     6181 gtatggaagg cggcgctcga gccgtaccgc acgaccaact tcgcggcgag gctggcggag
     6241 attcgtgtcc cgacactgct cgtctggggc gagcacgacg cgtttacgct gcgcgccgag
     6301 caggaggcgt tgacgcaggc cattccaggg gcaacgctcg tcgtctactc aggcaccggt
     6361 cactgcccgc actgggagga acctgagcgc tttgccgggg aggtcgcctc gttcatcgcg
     6421 cacgtggtaa gtattgaagg ttgacgaagg agga
//
LOCUS       DBNGSH010001059         9388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_675724, whole genome shotgun sequence.
ACCESSION   DBNGSH010001059 DBNGSH010000000
VERSION     DBNGSH010001059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_675724"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="AC16CY_23950"
     CDS             <1..165
                     /locus_tag="AC16CY_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3356928.1"
                     /translation="SLGGIPLGRPAKPREVAELIAFVTSPRAAAITGTEFVIDGGTGS
                     HRVKTGRAES"
     gene            complement(213..286)
                     /locus_tag="AC16CY_23955"
     tRNA            complement(213..286)
                     /locus_tag="AC16CY_23955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(252..254),aa:Gln,seq:ctg)
     gene            complement(431..1927)
                     /gene="gltX"
                     /locus_tag="AC16CY_23960"
     CDS             complement(431..1927)
                     /gene="gltX"
                     /locus_tag="AC16CY_23960"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3356929.1"
                     /translation="MAPRVRFAPSPTGFLHVGGARTALFNWLYARRHGGAFVLRIEDT
                     DTERSSEDMVTGILDGLRWLGLTWDEGPGIGGPHAPYFQSARLDRYRAAAMRLRDEGR
                     AYFCYCTPERLRDERQRAEARGEGWQYDRACLALTSNRVRELEDAGAPRAIRFKVPAG
                     GAAFDDLVHGRIAFDGANIEDFVILRSDLHPTYHLSVVVDDVEMGITDVIRGDDHISN
                     TPKHVLLFQALGAPLPRFAHVPLILGADKKRLSKRHGATSVMEYQRQGYLPPAMVNFL
                     ALLGWSPGDDRELMTEADLVASFTLEGISGGNAVFDTDKLDWMSGQYIARMAIEDLAS
                     AVLPFMADAGLAAHPLVTDAGAFHRLLELLRPRAKRLTEFAEQARPLLTEAVAYEPEA
                     VEKHLSSPDLPGHIAALVEALHGAAPFDEAQVEAAVRGTAAARGVKAGALIHATRVAV
                     TGRTQSPGLFEVLAWLGPERTRARLARLLEFLKRDVRSVRLQPDHERT"
     gene            2019..2489
                     /locus_tag="AC16CY_23965"
     CDS             2019..2489
                     /locus_tag="AC16CY_23965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356930.1"
                     /translation="MAHEPAALTTIRTILLWALVLGATGMAAELLLIGHDESASQLVP
                     LALLGAGVLLGASLIAAPSVWSLRLLQLLMVLFVGSGMLGVALHYQGNAEFELEMHPS
                     LSGMKLITGTMTGATPVLAPGSMSLLGVVGLAFTYRHPLLRSHAGVASTEEAQS"
     gene            2486..3130
                     /locus_tag="AC16CY_23970"
     CDS             2486..3130
                     /locus_tag="AC16CY_23970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356931.1"
                     /translation="MTRVLLVALLSAVLVLHARPASAQVGKSVTIADANTIGEADLAK
                     LPGMTPATAKAVVAKRPFLSVTALDQALTGAGLTREQITTLYGRIFIHINLNSAPNEE
                     ILLIPGLGKRMLGEFLEYRPYAALAVFHREIDKYVDDAELARLEQYVFVPMDLNTASD
                     ADFLTIPGLGNRMLREFKEYRPYDGIERFRREIGKYVSKEEVARLERYVTIAKK"
     gene            complement(3214..4701)
                     /locus_tag="AC16CY_23975"
     CDS             complement(3214..4701)
                     /locus_tag="AC16CY_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3356932.1"
                     /translation="MRWPVVFLSIAAALGVTAAAQHKAEQHDMALAGYSDLQARSAYQ
                     PTIHRQGDRWIAYVGHHGGSQVNPLTGRQEDNGTSIVDVTDPRRPQYLAHIPGEPGQG
                     ESGGAQMTRVCNGSALPRADKSKVYLLRSFGNSAHEIWDVSDPARPSRVTVVLDGLVN
                     THKNFWECDTGIAYVISGPREWRTRRMTKVYDLSDPARPVFIRDFGLPGQQPGATMQP
                     VPTELHGPISLGPKGNRVYFGYGTSRAGVVQIVDREKLLNGPKEPTDANLLYPQIARI
                     DLPPDAGAHTAFPLLGMTLTEFARQKLPANAPAPGGGHDHGGAVPLAATQAHRDFIAV
                     VSESLANECEEPRQLVRFLDVTFESRPIGVSTWTVPEASGNFCDRGGRFGAHSSNESF
                     TPIYYGRVLFVTFFNAGLRALDVRDPNSPKEIGYYIPATTERTDRRCVGQPPNERCKV
                     AIQSNNVEVDDRGYVYLVDRANTGMHILELTGAARRAANFPAGSN"
     gene            complement(4723..6144)
                     /locus_tag="AC16CY_23980"
     CDS             complement(4723..6144)
                     /locus_tag="AC16CY_23980"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3356933.1"
                     /translation="MPIHPRPTRIAALILVVSVSVTGVLAQTAIKLPKNKFTPQQDVQ
                     LGREAAAEIKEQYPIIKDERITKYLARLGDQLVAVAPSELKQPVYEYSFTPVNLKEIN
                     AFALPGGPMFVHRGMFDAAAAEGEVVGVMAHELSHVLLRHGTANVTKAQSPWLQLGQI
                     GGAIGGAVVGGAAGSLITQGAQFGLGLKFLSYSREFEKQADLLGAQIMARAGYDPRQL
                     ARMFETIEKESKSSGGGGPQWMSSHPNPGNRTQYITKEAGLLTVAKPADQSEFAPIKT
                     AFAAQPAAKTMGELAKAKSSGGGEAVKSVGTPGQPVPAPSSQYRTVSASKVFQASVPS
                     NWTNIPSSTAIKVVPENGYGELKGQTAFTHGVEFGVSKADTRDLQEATKTWLNAIAQS
                     NPDLRLAGKQQAIKISDRSALATPLVNASPLGGQEQIGVYTAFLADGTLFYFITIVPE
                     KEAANFQQAFQKIGQSIRLTDAR"
     gene            complement(6309..7670)
                     /locus_tag="AC16CY_23985"
     CDS             complement(6309..7670)
                     /locus_tag="AC16CY_23985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIR3356934.1"
                     /translation="MPDPIFQPLTINGLTLKNRVVRSSLGGRVDYYDGSMSNARIAWD
                     RRFARGGISAIISSNAGIRTDGIAVPGYASIDNDRTIPSWRQLVEEVHRDDCRYVIQL
                     HFSGRQRDLPRKEFVGVLAPSATDRPDLLYGLRSRRMTIREIADLVQDYARAARRARE
                     AGADGIEIVACNGYVLHQFLSGAINDRTDEYNGDLRARARILLEVLGAVRAAVGRDYF
                     VSMKLSGRDEHNAYTAPLNRRVGNTIDDTMQVSRWLADAGLDAIHVSQGDSFPHPLVP
                     AGLLPADESRHSMAAMFYEGRRVPTRFFLMQFALFRRFTEWNWGRRMPFKRRGVILPE
                     MIEGMNQDDAAKIKKASGLPVICVGGWQTASRIRDALSANRCDVVSIARGLLANPDLV
                     RRFATGQDAADKPCTYCNKCLVNALLQPLACWDESRFATREQMFEEAYRVYQEAAVGH
                     VSE"
     gene            7806..8312
                     /locus_tag="AC16CY_23990"
     CDS             7806..8312
                     /locus_tag="AC16CY_23990"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3356935.1"
                     /translation="MAKVATTVISTIAVPRRALYAWLVPGVFLNELDTVLRDEAGLPG
                     VTRTTGTTGPWDVAGSTRIVHLSDGTFCRETVRTATAPDYFAYRLTEFSSRLISMLVR
                     EANGQWWFTDDGTGTRAKWTYTAESRSFLATPILIPVIRIMWNRYMSTAMKHIKTRAE
                     KEVVGVSR"
     gene            8309..>9388
                     /locus_tag="AC16CY_23995"
     CDS             8309..>9388
                     /locus_tag="AC16CY_23995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356936.1"
                     /translation="MTQWDFDLVALHGSQEMTAEDRGYRGSRFAEVRAAIYENPYRGG
                     TSGQQPGPLPMFKSTIRNAWRGALFGENKFRQASARAIDSRADLRWGTDRKGWRRIIA
                     PNGICVLGDWEITADNPYSGYFRKGSKGLTIGRFSSDGNETKRGERRSFSLGMKIYPT
                     MDREHPAPLMPASVIVQEDLGGMHTDYINDAELVNAPSVHAYRRGIYFLIMLRAGPIF
                     QRLDKVGDSRQLHEVAELGKPPGEVTSAPEHMLLKMTPGQRRVEGRQLDFREEIYGHI
                     FKPGDGEPTGVMEFDISVSSYGRKTGVQGLSRVTVTDWQRIGKLRFTEAVASYNADHV
                     VQFHHPGWRDDRNDPSTYIRVNEQRV"
BASE COUNT         1540 a         3102 c         3118 g         1628 t
ORIGIN      
        1 tcgcttggcg ggattccgct cggacggccc gcgaagccgc gtgaggtcgc cgagctgatc
       61 gcctttgtga cctcgcctcg agcggctgcg atcaccggca ccgagttcgt catcgatggt
      121 gggactggtt cccaccgcgt gaagacaggt cgcgcggagt cttgaggcta cgagcgagcg
      181 aaaccgtgag gagcagccag ctctgaagga gctggctggg aggcagggat tcgaaccccg
      241 ataacgtggt ccagagccac gtgtcctacc gttgaacgac ctcccagtgt caggacttcc
      301 gcgggacgcg aacttccaac tatagctggc ccgccgcgtc ggtcgcaaga cgaggggtcc
      361 ggtccggctg aagccggaca ctacgtacga caccacgtac gctcacgctc cggctaaagc
      421 cggatggaca ctacgtgcgc tcgtggtccg gctgaagccg gacactacgt acgtcgcgct
      481 tgaggaactc gagcagccgc gccaggcgcg cgcgcgtccg ctccgggcca agccaggcca
      541 ggacctcgaa cagccccggg ctctgcgtcc gtcccgtcac ggccacgcgc gtggcgtgaa
      601 tcagcgcgcc cgccttgacc ccccgcgcgg ccgccgttcc gcggacggcg gcctcgacct
      661 gcgcctcgtc gaacggcgcc gccccgtgca gcgcctcgac gagcgccgcg atgtgccccg
      721 gcaggtctgg agaggacaga tgcttctcca cggcctcggg ctcgtacgcc acggcttcgg
      781 tgagcagggg ccgcgcctgt tcggcgaact cggtcagccg cttcgcgcgc gggcgcagca
      841 gctccagcag ccggtgaaac gcgccggcat cggtgaccag cggatgcgcg gcgagcccgg
      901 cgtccgccat gaacggcagc acggctgacg ccaggtcctc gatcgccatt cgcgcgatgt
      961 actggccgct catccaatcg agcttgtcgg tgtcgaagac ggcattgcct ccgctgatgc
     1021 cctcgagcgt gaagctcgcg acgagatccg cctccgtcat cagctcgcga tcgtccccgg
     1081 gcgaccatcc gagcagcgcc agaaaattca ccatcgccgg cggcaggtag ccctgccgct
     1141 ggtactccat caccgacgtc gcgccgtgac gcttgctgag gcgcttcttg tcggcgccga
     1201 ggatcagcgg cacgtgcgcg aagcggggga gtggagcacc cagggcctgg aacagcagca
     1261 cgtgcttcgg cgtgttcgag atgtggtcgt ctcctcggat cacatccgtg atccccatct
     1321 ccacatcgtc cacgacgacg gagaggtggt aggtggggtg gaggtccgac cggaggatca
     1381 cgaagtcttc gatgttggcg ccgtcgaacg cgatgcggcc gtgcaccagg tcgtcgaacg
     1441 ccgccccgcc ggcggggacc ttgaagcgga tcgctcgcgg cgcgcccgcg tcttcgagct
     1501 cacggacgcg gttgctcgtc agcgccaggc acgcgcggtc gtactgccag ccctcgccgc
     1561 gcgcttccgc gcgctggcgc tcgtcgcgca gccgctccgg agtgcagtag cagaagtacg
     1621 cgcgcccttc gtccctcaag cgcatcgccg cggcgcgata gcggtcgagc cgcgccgact
     1681 gaaagtaggg cgcatgcggt ccgccgatcc cggggccttc gtcccacgtg aggccgagcc
     1741 agcgcaggcc gtcgaggatc ccggtgacca tgtcctcgga cgagcgttcc gtgtcggtgt
     1801 cctcgatgcg aagcacgaag gcgccgccgt gccgccgcgc gtagagccag ttgaacaggg
     1861 ccgtgcgggc gccgccgacg tgcaggaacc cggtggggga cggcgcgaag cgcacgcggg
     1921 gggccaccga gccattatgc tactgtcacg atcacggtcc ggctcccgcc ttcgcgctgc
     1981 gcgctacggc gggcaagaaa gccggacact acgtacccat ggcacacgaa ccggcggcgc
     2041 tgacgaccat tcgcaccatc ctgctctggg cgctcgtact aggcgcgacg ggcatggcag
     2101 cggagctgct gctcatcggc cacgacgagt cggcgtcgca gctcgtgccg ctcgccctgc
     2161 tcggcgcggg cgtcctgctc ggcgcctcgc tgatcgccgc tccctccgtc tggagcctgc
     2221 ggctgcttca gttgctgatg gtcctgttcg tcggcagcgg tatgcttggc gtcgcccttc
     2281 actatcaggg caacgcggaa ttcgagctgg agatgcatcc gtcgctgtcg ggcatgaagc
     2341 tcatcacggg taccatgacc ggcgcgacgc ccgtgctcgc cccgggctcg atgtcgctgc
     2401 tcggcgtcgt tggtctcgcg ttcacgtacc gccacccgct gctgcgttcg cacgccggcg
     2461 tggcatcaac agaggaggct caatcatgac tcgggttctg ctggtcgcgc tgctcagcgc
     2521 cgttctcgtg ttgcacgccc gccccgcgtc cgcacaggtg gggaagtcgg tcaccatcgc
     2581 cgatgccaac acgattggcg aagcggacct cgcgaagctt ccgggcatga cgccggccac
     2641 ggccaaggct gtcgtcgcca agcgtccgtt cctctcggtg acggcgctcg accaggcgct
     2701 caccggcgcc ggcctcacgc gggagcagat caccacgctg tacggccgca tcttcattca
     2761 catcaacctg aacagtgctc caaacgagga gatcctcctc atccccggct tgggcaagcg
     2821 gatgctcggc gagttcctcg agtaccggcc gtacgccgcg ctggcggtct tccaccgcga
     2881 gatcgacaag tacgtggacg atgcggagct ggcgaggctg gagcagtacg tcttcgtgcc
     2941 gatggatctg aacacggcga gcgacgcgga cttcctgacg attcccggtc tcggcaaccg
     3001 gatgctgcgc gagttcaagg agtaccgccc ctacgacggc atcgagcggt tccgccgcga
     3061 gatcgggaag tacgtgagca aggaagaagt ggcgcggctg gaacgctacg tcacgatcgc
     3121 gaagaagtag ggtctccggg cgatttcgtc gcggtcgcgg cccggtctgc gcgtctcctc
     3181 gcccacggtc gtttcgcggc cgcggctacg ctctcagttc gagccggccg ggaaattcgc
     3241 ggcccgccgc gcggcgccgg tgagctccag gatgtgcatg ccggtgttcg cgcgatcgac
     3301 caggtagacg tagccccggt cgtctacctc cacgttgttc gactggatcg ccaccttgca
     3361 gcgctcgttc ggcggctggc cgacgcagcg ccgatcggtc cgctcggtag tggccgggat
     3421 gtagtagccg atctccttgg ggctgttcgg atcgcggacg tcgagggcgc gaaggccggc
     3481 gttgaagaac gtcacgaaca gcacgcggcc gtagtagatc ggggtaaagc tctcgttcga
     3541 cgagtgcgcg ccgaagcgcc cgccgcggtc gcagaagttc ccgctcgcct cgggcacggt
     3601 ccacgtcgac acaccgatcg gacgcgattc aaacgtcacg tcgaggaagc gaacgagctg
     3661 gcgcggctcc tcgcattcgt tggccagtga ctcgctcacg accgcgatga aatcgcggtg
     3721 cgcctgcgtc gccgcgagcg gcaccgcgcc gccgtgatcg tgtccgcctc ccggcgccgg
     3781 tgcgttcgcc ggaagcttct gtctcgcgaa ttctgtcagc gtcatcccga gcagcggaaa
     3841 ggccgtgtgg gcgccggcat cgggtggaag atcgatccgt gcgatctggg gatagagaag
     3901 gttggcgtcg gtcggctcct tcggtccgtt gagcagcttc tctcgatcga cgatctgcac
     3961 gacgccggcg cggctcgtac cgtagccgaa gtagacgcga ttgcccttcg gccccagcga
     4021 gatcggtccg tgcagctcgg tgggcaccgg ctgcatcgtc gcgccgggct gctggccggg
     4081 caggccgaag tcgcgaatga acaccggccg cgccggatcg ctcaagtcgt agaccttcgt
     4141 catcctgcgc gtgcgccact ctctcggccc ggagatcacg taggcgatgc cggtgtcgca
     4201 ctcccagaag ttcttgtgcg tgttcacgag gccgtcgagg acgacggtga cgcgcgaggg
     4261 cctggccggg tcgctcacgt cccagatctc atgcgcggag ttaccgaagg agcgaagcag
     4321 gtacaccttg ctcttgtcag ctcgaggcag cgcgctgccg ttgcagacgc gcgtcatctg
     4381 cgcgccgccg ctttctccct gcccgggctc gccgggaatg tgggcgaggt actgagggcg
     4441 ccgcggatcg gtgacgtcca cgatcgacgt accgttgtcc tcctgccgtc ccgtcagcgg
     4501 gttgacctgg ctgccgccgt ggtgcccgac gtaggcgatc catcgatcgc cctgtcggtg
     4561 aatcgtcggc tgatacgcgg accgggcctg caggtcgctg tagcctgcga gcgccatgtc
     4621 gtgttgctcc gccttgtgct gcgcggcggc ggtgacgcca agggctgcgg caatcgacag
     4681 gaagacgaca ggccatcgca tgacggttct cctgcaggag gattagcgcg cgtccgtcag
     4741 gcgtatcgac tggccgatct tctggaacgc ctgctgaaaa ttcgccgcct ctttctcggg
     4801 cacgatcgtg atgaagtaga agagcgtccc gtccgcgaga aacgcggtat acacgccgat
     4861 ctgttcctgg ccgccaagcg gcgacgcgtt gacgagcggc gtcgccaggg ccgagcggtc
     4921 cgaaatcttg atcgcctgct gcttgcccgc aagacgaaga tcgggattgc tctgggcgat
     4981 ggcattcagc cacgtctttg tcgcttcctg caggtcgcgc gtgtccgcct tggagacgcc
     5041 gaactcgacg ccgtgggtga acgccgtttg tcccttgagc tcgccgtatc cgttttccgg
     5101 gacgaccttg atcgcggtgc tcgaaggaat gttggtccag ttgctcggaa cggacgcctg
     5161 gaagaccttg ctggcgctga cggtccgata ctgcgaagac ggtgccggca caggctgtcc
     5221 gggggttccc actgatttca cggcctcgcc gcccccgctg ctcttggctt tcgccagctc
     5281 gcccatcgtc tttgccgccg gctgtgccgc gaaggcggtc ttgatcggcg cgaactcgct
     5341 ctgatcggcc ggcttggcga cggtcagcag gccggcttcc ttggtgatgt actgcgtccg
     5401 gttgccgggg ttgggatggc tgctcatcca ctgcgggccg ccccctccgc tgctcttcga
     5461 ctccttctcg atcgtctcga acatgcgggc gagctggcgc gggtcgtagc cggcgcgcgc
     5521 catgatctgc gcgccgagca ggtcggcctg cttttcgaat tcgcggctgt aactcaggaa
     5581 tttcaggccc aggccgaact gggcgccctg cgtgatgagc gaaccggccg cccctccgac
     5641 caccgcgccc ccgatcgcgc ccccgatctg tccgagctgc agccaggggc tctgcgcctt
     5701 ggtcacgttg gccgtgccgt gccgaagcag gacgtgtgac agctcgtgcg ccatcacacc
     5761 caccacctcg ccctccgcgg cggcggcgtc gaacatgccg cgatggacga acatcggccc
     5821 gcccggcaat gcgaaggcgt tgatctcctt caggttcacc ggcgtgaacg agtactcgta
     5881 gacaggctgc ttcagctctg agggggcgac agccaccagt tggtcgccga gccgggcaag
     5941 gtacttggtg attcgttcgt ccttgatgat gggatactgc tccttgatct cagcggcggc
     6001 ctcgcggcca agctggacgt cctgctgcgg cgtgaacttg ttcttcggca gcttgatcgc
     6061 cgtctgcgcg agcacgcccg tcaccgacac ggatacaacc agtatgagag ccgcaatgcg
     6121 cgtaggccgg gggtggattg gcatgacctc tctccttgat cgacgggctt ccatcctagc
     6181 gtgaaagcaa gcatcgagcc atcggcggcg cgcggaagca gagcaggagc gtttcgagac
     6241 agcacgaacc ctcgagctgc agccctgctc gcgacgctct gcctcacatc gaaacctccg
     6301 ggcttcactc actccgacac gtgacctaca gccgcctcct gatagacgcg gtacgcctcc
     6361 tcgaacatct gctcgcgcgt ggcgaaacgg ctctcgtccc agcaggccag cggctgcagg
     6421 agggcgttga cgaggcactt gttgcagtac gtgcagggct tgtctgccgc gtcctgtccg
     6481 gtcgcgaacc gtcgtacgag gtccgggttc gccagcaggc ctcgcgcgat cgagacaacg
     6541 tcgcagcggt tggcggacag cgcatcgcgg atccggctgg ctgtctgcca gcccccgacg
     6601 cagatcaccg gcaggcccga ggccttcttg atcttcgccg catcgtcctg gttcatcccc
     6661 tcgatcatct ccggcagaat cacgccgcgg cgcttgaagg gcatccgcct gccccagttc
     6721 cattcggtga agcggcgaaa cagcgcgaat tgcatcagga agaacctggt cggaacccgc
     6781 cgcccctcgt agaacatggc cgccatgctg tgccgggact cgtcggcagg aagcaacccg
     6841 gccggaacga gcgggtgcgg gaacgaatcc ccttgagaca catggatggc gtcgagtccc
     6901 gcatccgcca accagcgtga gacctgcatg gtgtcgtcga tggtgtttcc gactcgacgg
     6961 ttgagcggag cggtgtacgc gttgtgctcg tccctgccac tcagcttcat cgacacgaaa
     7021 tagtcgcggc cgaccgcagc gcggacagcg ccgaggacct ccagcagaat ccgcgcgcgt
     7081 gccctcaggt cgccgttgta ctcatcggtc cggtcgttga tggcgccgga gaggaactgg
     7141 tgaaggacgt aaccgttgca cgcgacgatt tcgatgccgt cggcgccagc ctcacgtgcc
     7201 cggcgcgcgg cgcgtgcgta gtcctgcacc aggtcggcaa tctcgcgaat agtcatgcgg
     7261 cgagagcgca gtccgtacag gagatcgggc ctgtcggtcg cgctcggcgc cagcacgccg
     7321 acgaattcct tgcggggcag gtcgcgctgc ctgccggaaa agtgtagttg gatgacgtac
     7381 cggcaatcgt cgcggtgcac ttcttcgacg agttgccgcc acgacgggat cgtgcggtcg
     7441 ttgtcgatgc tcgcgtatcc cggcacggcg attccgtcag tccggatgcc cgcgttggac
     7501 gagatgatgg cgctgatgcc gccacgagcg aagcggcgat cccacgcgat ccgggcgttg
     7561 gacatcgagc cgtcgtagta atcgactcgt cctcccaggc tcgatcgcac gactctgttc
     7621 ttgagcgtca acccgttgat ggtcagcggc tgaaagattg gatcgggcat ggcgggccca
     7681 ttctaggagc gcgagcagaa gggcgcggca aaagaaaatg cgcaaggctg agcttgcgat
     7741 gtgtgcgcgt actcgtatga tggctccccc cgaagcaata cgaatcgaat caaaggtgga
     7801 gcctgatggc gaaggtagcc acgacagtaa tctcgacgat tgctgtgccg agacgagcgc
     7861 tctatgcctg gctcgtcccg ggcgtgttcc tcaacgagct cgacacggtc ctgcgcgatg
     7921 aggcgggact gcctggtgtc acgcgaacca cgggcacgac cggcccctgg gacgtggccg
     7981 gctcgacgcg catcgtccat ctgtcggatg ggacgttctg ccgggagacg gtgaggaccg
     8041 cgactgctcc agactatttc gcgtaccgcc tgacggagtt ctcgagccgg ctgatcagca
     8101 tgctcgtgcg cgaggccaac ggacagtggt ggtttaccga cgacggcacc ggcactcgcg
     8161 cgaagtggac ctacaccgcc gagagccggt ccttcctggc gacccctatc ctcattccgg
     8221 tgatcaggat catgtggaac cgctacatga gcacagcgat gaagcacatc aagacgcgcg
     8281 ctgagaagga agtcgtgggg gtttcgcgat gacgcagtgg gatttcgatc tcgttgccct
     8341 gcacggttcg caggagatga cggcggagga tcgcggctat cgcgggtctc gattcgccga
     8401 ggtccgcgcg gcgatttacg agaaccccta tcgcggcggc accagcggtc aacagccggg
     8461 gccgctgccg atgttcaagt cgaccattcg caacgcctgg aggggtgcgc tgttcggcga
     8521 gaacaagttc cgtcaggcgt cggcgcgagc gatcgattca cgggccgacc tccgctgggg
     8581 caccgatcga aagggatggc gccgaatcat cgcgccgaac ggtatttgcg tgctcggcga
     8641 ttgggagatc accgccgaca atccgtattc cggctacttc aggaagggct cgaagggact
     8701 cacgatcggc cggttctctt ccgacggcaa tgaaacgaag cgcggggagc gtcgctcgtt
     8761 cagcctcggc atgaagatct acccgacgat ggaccgcgag catccggcgc cgctgatgcc
     8821 ggcaagcgtg atcgtccagg aagacctcgg cggcatgcac accgactaca tcaacgacgc
     8881 cgagctggtc aacgcgccca gcgtccacgc ctaccggcgc gggatctatt tcctgatcat
     8941 gctgcgcgcg ggcccgattt ttcagaggct cgacaaagtc ggcgacagcc ggcagctcca
     9001 cgaagtcgcc gaactcggca agcctcccgg cgaggtcacg agtgcccccg agcacatgct
     9061 gctcaagatg acgccgggac agcgccgcgt cgagggacgc cagctcgatt tcagagagga
     9121 gatctacggc cacatcttca agcccggcga cggggaaccc actggcgtga tggagttcga
     9181 catctctgtg tccagctacg ggcggaagac cggcgtccag gggctgtcgc gcgtgacggt
     9241 gaccgactgg cagcggatcg gcaagttgag gttcacagag gcggtggcct cctacaacgc
     9301 cgaccacgtc gtccagttcc accatcccgg ctggcgggac gaccgcaacg atccgtccac
     9361 ctacattcgc gtgaacgagc aacgcgtg
//
LOCUS       DBNGSH010001060         1985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3731165, whole genome shotgun sequence.
ACCESSION   DBNGSH010001060 DBNGSH010000000
VERSION     DBNGSH010001060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3731165"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..>1985
                     /locus_tag="AC16CY_24000"
     CDS             49..>1985
                     /locus_tag="AC16CY_24000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3356937.1"
                     /translation="MAERPDTPNAWVTAVRRLPERDARYGMHVEGLDARLLEVLKERG
                     VERFYVHQSEAIEHALARRHVVITTPTASGKTLCYNAPVLSSILRDPSTRALYLFPTK
                     ALAQDQLAELHELSDRLSRRTDLEIGVFTYDGDTPQDARRAIRGRAHIVLSNPDMLHS
                     GILPHHPRWAKLFENLQYVVIDELHAYRGVFGSHLANVLRRLQRVCRHYGSNPAFICS
                     SATIANPRELAEGLVEQPFVLVSESGAPRGEKWFLFVNPPVVNAQLGIRRSYLAETRR
                     VAAEFLKRSLQLIVFAQSRLTTEILTTYLKDDFEGGAGTQDRIRGYRGGYLPQRRREI
                     EKGLRDGSVRAVVSTNALELGIDIGALDVCVMAGYPGTIAATWQRAGRAGRRSGRSAA
                     VLVASSAPLDQFVVRNPSYFFDAPPEHALINPDNLHILVDHVKCAAFELPFTTAERYG
                     RVDVQNVLDVLEESGFVHRTSGDGEDGVWQWTNESYPADAVSLRSISSDNFVVIDTTS
                     GADVIGETSFTSGPATLHEKAIYLVEGVLYQVEKLDFVGRKAYVRRIDCDYYTDAITY
                     SKVKILETFVPDGTAAHGEVHVSSRVVGFKKIKFYTNENVGSGELDLPEQQMHSTAYW
                     LTVPRAVVAALPFASDDRRDGV"
BASE COUNT          340 a          645 c          671 g          329 t
ORIGIN      
        1 ggcggggatc gggcgctcgt ggtggtgctc gacacgctga ccggcggcgt ggccgaacgg
       61 ccggacacgc caaacgcgtg ggtcactgcc gtgcgcaggc ttccggagcg cgacgcgcga
      121 tacggaatgc acgtcgaggg gctggacgca cgtctgctcg aggtgctgaa ggaacggggc
      181 gtcgagcggt tctacgtcca tcagtcggag gcaatcgaac acgccctggc gcggcgccac
      241 gtggtcatca ccacgcccac cgcatcgggc aagacgctgt gctacaacgc gccggtcttg
      301 agttctatcc tgcgcgaccc gtccacgcgg gcgctctacc tgttcccgac caaggcgctc
      361 gcgcaggatc aacttgccga acttcacgag ctgtccgatc gcctttcacg ccggaccgat
      421 ctggagattg gcgtgttcac ctacgacggc gacacgccgc aggacgcgag gcgcgcgatt
      481 cgcggacgcg cgcacatcgt gctcagcaac cccgacatgc tgcactcggg gatccttccg
      541 catcacccgc ggtgggccaa gctgttcgag aacctccagt acgtcgtgat cgacgagctg
      601 cacgcgtacc gcggagtctt cggcagccat ctcgcgaacg tgctgaggcg gctccagcgg
      661 gtctgccggc actacggctc gaatccggcg ttcatctgct cgtcggcgac gattgccaat
      721 cctcgcgagc ttgcggaagg cctcgtcgag cagcccttcg tgctcgtgtc cgagagcggc
      781 gcgccgcgcg gggaaaagtg gttcctgttc gtgaacccgc cggtggtcaa cgcgcagctc
      841 gggattcgcc ggtcctacct ggccgagact cgccgcgtgg cggccgagtt cctgaagcgg
      901 tcgctgcagc tcatcgtgtt cgcccagagc cggctgacga cggagatcct gacgacgtat
      961 ttgaaggacg acttcgaagg gggcgccggc acgcaggatc gcattcgcgg ctatcgaggc
     1021 ggctacctcc cgcagcgccg gcgtgaaatc gaaaagggcc tgcgcgacgg cagcgttcga
     1081 gccgtggtgt cgaccaacgc gctcgaactc ggcatcgaca tcggcgcgct cgacgtgtgc
     1141 gtcatggcgg gctatccggg aaccatcgcg gccacgtggc agcgagcggg tcgcgcgggc
     1201 cggcgcagcg gacgatcggc ggcggtgctg gtcgcatcga gcgctcccct cgatcagttc
     1261 gtcgtgcgca acccgtcgta cttcttcgac gctcctcccg agcacgcgtt gatcaacccg
     1321 gacaacctgc acatcctggt ggatcacgtg aagtgcgccg ccttcgagct gccctttacg
     1381 accgccgagc ggtacggccg ggttgacgtg cagaacgtgc tcgacgtgct cgaggaaagc
     1441 ggtttcgtgc accggacctc cggcgacggg gaagacggcg tgtggcagtg gaccaacgag
     1501 tcgtatcccg ccgacgccgt cagcctgcga tcgatctcgt cggacaactt cgtcgtgatc
     1561 gatacgacga gcggcgcgga cgtgatcgga gaaacgagtt ttacgagcgg gccggcgacg
     1621 ctgcacgaga aggccatcta cctggtcgag ggcgtcctgt accaggtgga gaagctggac
     1681 ttcgtggggc gcaaggccta cgtcaggcgc atcgactgcg attactacac cgacgccatc
     1741 acgtattcca aggtcaagat cctcgaaacg tttgtcccag acggcacggc ggcgcacggc
     1801 gaagtccacg tgtcatcgcg cgtcgtcggg ttcaagaaga tcaagttcta caccaacgag
     1861 aacgtcgggt ccggcgagct cgacctgccg gagcagcaga tgcactcgac cgcgtactgg
     1921 ctgacggtgc ccagggccgt ggtggcggcg cttccgttcg cgtccgacga ccggcgggac
     1981 ggtgt
//
LOCUS       DBNGSH010001061         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_2713039, whole genome shotgun sequence.
ACCESSION   DBNGSH010001061 DBNGSH010000000
VERSION     DBNGSH010001061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2713039"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="AC16CY_24005"
     CDS             complement(<1..469)
                     /locus_tag="AC16CY_24005"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3356938.1"
                     /translation="MFSNRREFIVRSSMAVAAGALGSSRVLAQQPAAAPAAPTVPQFA
                     SLRRNVGTFTARGGTMGWLVAPDAVVVIDSQFADTSKMFLDGFKPMIGGRKIDLLINT
                     HHHPDHTGGNKVLQPSVVKIVAQENAPNLQRKQAAAQKSEDNQAYADTTFKSDW"
     gene            complement(551..1555)
                     /locus_tag="AC16CY_24010"
     CDS             complement(551..1555)
                     /locus_tag="AC16CY_24010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3356939.1"
                     /translation="MRTWIAPAGCKSTSELRIVERPDPAPASTQVVVRVRATSLNYRD
                     QLVTSGHYFGGVLSRDTIPLSDGAGEVTAIGPGVSRFKPGDRVAATFFQPVPAGAPRA
                     ALGTPLDGMLSEYVMLPEDGLVPIPAHLSYEQAACLPCAGVTAWHALFRAGRPVTAAS
                     TVMVLGTGGVSIAALQFARSAGARVIATSSSDEKLARVKALGAADGINYTRTPEWAQE
                     VRRLTGGRGVDCVVEIGGAGTLSRSMQSLAPFGKICLIGFLAGREGDTNPMPLMLIGG
                     SLHGIFVGDREMFEEMNRAIETNRIAPVVDRVFPFDEAPAAYRHHASGNFVGKVVIAV
                     "
     gene            complement(1569..>2483)
                     /locus_tag="AC16CY_24015"
     CDS             complement(1569..>2483)
                     /locus_tag="AC16CY_24015"
                     /inference="COORDINATES: protein motif:HMM:NF015863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HIR3356940.1"
                     /translation="WRGWLRQPQRVWLRRAAFQVHLWIGLAIGLYIVMLSITGSALVY
                     RNELDRFFATPRPPYDPKGTVLTSDQLRAIAQRLYPDYTITHVGERISRRNPTLEVGF
                     ERGAEKKQRLFNPYTGEELGDSVTRGEYFVLWLARLHDELLFGHPGRFWNGVLSAVAT
                     VLFLTGLVVWWPGIGKWRRNLIVKPGVGWKRFNWDLHSAMGFWLFLFMLMWGISGFYL
                     GVPEPFSYIVDKVSDPDAFLGERPGDVVLSWLARLHFGRWRNMPWLKAFWAVVGLVPA
                     LMFVTGVVMWWNRVLRRRPIREVEESAA"
BASE COUNT          450 a          860 c          785 g          388 t
ORIGIN      
        1 tccagtcgga cttgaacgtc gtgtcggcat acgcctggtt gtcctcgctc ttctgcgcgg
       61 ccgcctgctt ccgctggagg ttcggcgcat tctcctgcgc cacgatcttg acgaccgagg
      121 gttgaaggac cttgttcccg ccggtgtgat ccggatggtg gtgcgtgttg atcagcaggt
      181 cgatcttccg gcctccgatc atcggcttga aaccgtcgag gaacatcttc gacgtatccg
      241 cgaactggct gtcgatgacg actacggcat ccggcgccac gagccagccc atcgtcccgc
      301 cgcgggccgt gaaggtcccg acgttgcggc gcaacgaggc aaactgaggc accgtcggcg
      361 ccgcgggcgc tgcagccggc tgctgcgcga gcacccggct cgagccgagc gcgccggccg
      421 ccacggccat cgacgaccgc acgatgaact cacgccgatt ggagaacata cagcacgctc
      481 ctttcccagt ccgtagtgtc cggcttcagc cgtagtacgt agtgtccggc ttcagccgga
      541 cccgttgcgg tcacaccgcg atgacgactt ttccgacgaa gttccccgac gcgtggtgcc
      601 tgtatgccgc cggcgcttcg tcgaacggaa acacgcgatc gacgaccggc gcgatgcgat
      661 tggtctcgat cgcgcggttc atctcttcga acatctcccg atcgccgacg aagatgccat
      721 gcaggctccc gccgatcagc atcagcggca tcggattggt gtcaccctcg cgacccgcca
      781 ggaagccaat caggcagatc ttgccaaagg gcgccagcga ctgcatcgac cgggacagcg
      841 tgcccgcacc cccgatctcg acgacgcaat cgaccccgcg tccgcccgtc agccgccgga
      901 cctcttgcgc ccactccggc gtgcgagtgt agttgatccc gtcagcggcg cccaaagcct
      961 tcacgcgcgc gagcttctcg tcactcgacg aggtggcgat gacgcgggca ccggccgatc
     1021 gcgcaaactg cagggcggca atcgatacgc ctcccgtgcc caggaccatc accgtcgatg
     1081 ccgccgtcac cggccgcccg gcgcggaaga gcgcatgcca cgccgtcacc ccggcgcaag
     1141 gcaggcaggc ggcctgttcg taggagaggt gggccggaat cggaaccagc ccgtcctccg
     1201 gcagcatgac gtattcggag agcatgccgt cgagcggcgt gccgagggcc gctcgaggcg
     1261 cgccggcagg aaccggctgg aagaacgtcg cggccacgcg gtcaccaggc ttgaaacgcg
     1321 acacgcctgg gccgattgcg gtcacctcgc ccgcgccatc cgacagggga atggtgtcgc
     1381 gactgaggac gccgccgaag tagtggcccg atgtgacgag ttgatcgcga tagttcagcg
     1441 aggtcgcacg cacgcgcacg acgacctgtg tcgacgccgg cgccggatcc ggacgttcga
     1501 cgatcctcag ctcgctcgtg ctcttacatc cagcgggggc gatccatgtg cgcatcttgg
     1561 tccttgggct acgccgcaga ctcctcgact tcccgaatcg gccggcgccg aaggacgcga
     1621 ttccaccaca tcaccacgcc cgtgacgaac atcagcgccg ggacgaggcc cacgacggcc
     1681 cagaaggcct tgagccacgg catgtttcgc catcgcccga agtgcaggcg cgcgagccac
     1741 gacagcacga cgtccccggg ccgctcgccg aggaaggcgt ccgggtccga gaccttgtcc
     1801 acgatgtacg agaagggctc cggaacaccg aggtagaaac cggatatgcc ccacatcagc
     1861 atgaagagaa acagccagaa gcccatggcg ctgtggagat cccagttgaa gcgcttccag
     1921 ccgacgccgg gcttcacgat gaggttgcgc cgccatttcc cgatccccgg ccaccagacg
     1981 acgaggcccg tcaggaacag gacggtggcg acggcactca acacaccgtt ccagaatcgt
     2041 cccgggtggc cgaaaagcaa ctcgtcgtgc agacgcgcga gccagagcac gaagtactca
     2101 cccctggtga ccgaatcgcc cagctcttcg cccgtgtacg gattgaacag ccgctgcttc
     2161 ttctcggcgc cgcgctcgaa ccccacctcg agcgtggggt tccggcgcga gatcctctct
     2221 ccaacgtggg tgatggtgta gtcgggatac aggcgctggg caatggcacg aagctggtcg
     2281 ctcgtcagca ccgtcccctt cgggtcgtat ggcgggcgcg gtgtcgcgaa gaaacggtcc
     2341 agctcgttcc ggtagacgag cgcgctgccc gtgatactga gcatgacgat gtagaggccg
     2401 atggcgaggc cgatccacag atgcacttga aacgccgccc gccggagcca gacccgctgc
     2461 ggttgccgca gccaccctcg cca
//
LOCUS       DBNGSH010001062         4155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_168672, whole genome shotgun sequence.
ACCESSION   DBNGSH010001062 DBNGSH010000000
VERSION     DBNGSH010001062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_168672"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="AC16CY_24020"
     CDS             <1..1061
                     /locus_tag="AC16CY_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356941.1"
                     /translation="VTGGFALTSSNNYDYAFERMVRENSSYYVLAFNSGAPRDGRYVP
                     IEVRVKRPGLQVRTMEGYVAPRGRPQEPRRPRTVLAAVWDAVASAVTTSGVSMRVSAA
                     PFRNRDGGKNATVAITLEIAPDKLNLVEQDGAYRGVLEILFATTDVKNKKWPIWRHRA
                     ALALKPETYERVSHGAIRVLSQLSLPEGRYQIRSSAGGAALAGSVVYDVVVPDFRDDF
                     SLSGIAVTSSQASETFTFTPHKKLEMSFPAPPTTAREFSRDDKLTLFAEAYENRKKPH
                     TVTLTIELHDEAGQVLDSHVMERKSADKPKQASVYTFSPYLDLEVVPPGRYAIRVVAR
                     STLDRNKTVSRDVPFSVR"
     gene            1155..3050
                     /locus_tag="AC16CY_24025"
     CDS             1155..3050
                     /locus_tag="AC16CY_24025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3356942.1"
                     /translation="MGLDTLLARTWVRSYDTITSTLSKRLCYGLYDGDRLEAHRRAKG
                     EVQVLFCIPPMSDVNGALPHPGVQILQTILREQGFSCEVLNYNLPCIHPRDPFDHLIR
                     AIKALGVRVLGVSTYSQAIRNTLEGLQRVRAACPDVHVVLGGPHPTESYLSVMGVSFV
                     DYVCRSEAEKSFPALVAALLSGRRPGACEIPGVYLRDPETNTVQGVQAGFVDLDALDQ
                     RKLLRYHFTRDELRQQRLYRGAHGTAGAEYWPVALVRGCPYDCTFCAAYQMSGKRLRY
                     RRVDQVVDDLEFYAQEYGRRHFSFIDDAFTQHYDYVIDLCNEILRRNLRVYWTTDNGI
                     RYETLGGGKLVEGVLKKRGLAGVDELISLMIRAGWRGTAIGIESGSTRVRTELVRKGG
                     VNLTNAEIIDNLTRLKQISKREGVYFYINGFLMAGFPELPLPNGKVVPVETEEEMEIT
                     RRFALELRDAGAIDMMNLSMVIPLPGTDMWECLSIRQKMQVLTSAVPPSDPSAPAIRD
                     IVARVLAAYPNPDATRYIEEPERRFWSEVYKLPDAAQILIMQSYDAFNADAAQNIELK
                     RPAPELLWRYRESVVGEFYGGLSMKLKMIGHVLRRSSGLHDAAAYMTLMGRKYDPEAK
                     MRPVATV"
     gene            3210..4097
                     /locus_tag="AC16CY_24030"
     CDS             3210..4097
                     /locus_tag="AC16CY_24030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HIR3356943.1"
                     /translation="MHTRIASRSRGRGRQARPCAILAFLIVVCSAPLAGQSSATRLTA
                     PAFEVASVKPSKDDRPPFFSTRPQGTVVITGQELRTIVALAYGIDLRVARFVLRGGPE
                     NILSARFDITGKPPDDGPPGQHLLMLRTLLAERFRLRSHTETRQMPIYALTVARKGTL
                     GPSLQLSSHDCVAFVAAGGKRTDADAPVDSSNRPLCWGTYGFAEAGPGTESMRYAGPL
                     AALVERLQPMLDRPVVDMTGLKGSYEWRLTFARDPRTDSEFPPIFTAVDEQLGLSLVA
                     RTGPVEVLVIDSVDPPVPD"
BASE COUNT          753 a         1389 c         1301 g          712 t
ORIGIN      
        1 cagtgacagg aggctttgcg ctgaccagct cgaacaacta cgactacgcg ttcgagcgga
       61 tggtccggga aaacagctcc tattacgtgc tcgccttcaa ttccggcgcc ccgcgcgacg
      121 gccgatacgt cccgatcgag gttcgcgtga agcgtcccgg cctgcaggtg cggacgatgg
      181 aaggctacgt ggccccgcgc ggcagacccc aggagccgcg acggcctcgc acggtcctcg
      241 cggcggtctg ggacgcggtc gcgagcgcgg tcacgacgag cggcgtgtcg atgcgcgtct
      301 ccgcagcacc gttcaggaac agggacggcg gcaagaatgc caccgtggcc atcacgctcg
      361 aaatcgcgcc agacaagctc aatctcgtcg agcaggacgg ggcgtaccgc ggcgtactcg
      421 agattctctt cgcgaccacc gacgtcaaga acaagaagtg gcccatctgg aggcaccgcg
      481 cggctctggc tctgaaaccc gagacctacg agcgggtcag ccacggcgcg attcgggtgc
      541 tgtcgcagtt gtcgttgccg gaggggaggt atcagatccg ttcatccgcg ggcggcgcgg
      601 ccctggccgg gagcgtcgtc tacgacgtcg tggtccccga cttccgggac gacttctcgt
      661 tgagcgggat cgcggtgaca tcatcgcagg cgagcgagac attcacgttc accccccaca
      721 agaaactcga gatgtcgttc cccgcacccc cgaccacggc gcgcgagttc tcgcgcgacg
      781 acaagctgac gctgttcgcg gaagcgtacg agaaccggaa aaagcctcac accgtgacgc
      841 tgaccatcga gcttcacgac gaagccggcc aggtgctcga cagccatgtc atggagcgga
      901 agtcggccga caagccgaag caggcgagtg tctacacgtt ctctccctat ctcgacctgg
      961 aggtcgtacc cccagggcgc tacgcgattc gtgtggtcgc gcgatcgacg ctggacagga
     1021 acaagaccgt gagccgcgac gtcccgttca gcgtccgctg acctacctga tccctgaacc
     1081 ctaactgcta acccctgccc cctgccccct gccccctgcc ccctgccccc tgccccctgt
     1141 atgattgctc caccatgggc ctcgatacgc tgctggcgcg cacctgggtc cgctcgtacg
     1201 acaccatcac gtcaacgctg agcaagcggc tctgctatgg gctgtacgac ggcgaccggc
     1261 tcgaggccca tcgccgcgcg aagggcgagg tgcaggtgct cttctgcatt ccgccgatga
     1321 gcgacgtcaa cggggcgctt ccccacccgg gcgtgcagat cctccagacg atccttcgcg
     1381 aacagggctt cagctgcgag gtgctcaact acaacctccc ctgcattcac ccgcgcgacc
     1441 cgttcgacca tctcatccgc gccatcaagg cgctgggcgt gcgcgtcctc ggcgtctcga
     1501 cctacagcca ggccatccgc aacacgctcg aagggctgca gcgggtgcgt gcggcgtgcc
     1561 cggacgtgca cgtcgtcctc ggcggcccgc atcccacgga gtcgtacctc agcgtgatgg
     1621 gcgtgagctt cgtggactac gtgtgccgga gcgaggccga aaagagcttc cccgcgctcg
     1681 tcgccgcgct cctgtccggg cgccggcccg gcgcgtgcga gatcccgggc gtctacctcc
     1741 gcgacccgga gacgaacacg gtgcagggcg tccaggcggg gttcgtcgat ctggacgcgc
     1801 tcgaccagcg caagctgctc cgctatcact tcacgcgcga cgagcttcgc cagcagcgtc
     1861 tctatcgcgg cgctcacggc acggcgggcg ccgagtactg gcccgtcgcg ctcgtgcgcg
     1921 gctgccccta cgactgcacg ttctgcgccg cgtaccagat gtcgggcaag aggctccgct
     1981 accgccgcgt cgaccaggtc gtggacgatc tcgagttcta cgcacaggag tacgggcgcc
     2041 gccatttcag cttcatcgac gatgcattca cgcagcacta cgactacgtg atcgacctct
     2101 gcaacgagat cctgcggcgc aatctgcgcg tctactggac gacggacaac ggcatccgct
     2161 acgagacgct cggcggcggc aagctcgtcg agggcgtgct gaagaagcgt ggtctcgcgg
     2221 gagttgacga gctgatctcg ctgatgatcc gcgcgggctg gcgcggcacg gccatcggga
     2281 tcgagtcggg ctcgacgcgc gtccgcaccg aactggtgcg caaggggggc gtcaatctca
     2341 ccaacgccga gatcatcgac aacctcacgc ggctgaagca gatctcgaag cgggaagggg
     2401 tgtacttcta catcaacggc ttcctcatgg ccggcttccc cgagctgccg ctgccgaacg
     2461 gcaaggtcgt tcccgtcgag accgaagagg aaatggagat cacgcggcgg ttcgctctcg
     2521 agctgcggga tgcgggcgcc atcgacatga tgaacctgag catggtgatt ccgctgccgg
     2581 gcaccgacat gtgggaatgc ctgtcgatcc gccagaagat gcaggtgctc acgagcgccg
     2641 tgccgccgtc ggacccctcg gcgccggcaa ttcgcgacat cgttgccagg gtcctcgccg
     2701 cgtacccgaa ccccgacgcc acgcgctaca tcgaggagcc cgaacgccgc ttctggtccg
     2761 aggtctacaa gctgcccgac gccgcgcaga tcctcatcat gcagtcctac gacgccttca
     2821 acgccgacgc ggcgcagaac atcgagctga agcggccggc gcccgagctg ctctggcgct
     2881 accgcgaaag cgtggtcggc gagttctacg gcggcctgtc gatgaagctg aagatgatcg
     2941 gccatgtcct gcggcgaagc agcgggttgc acgatgcggc cgcgtacatg acgctcatgg
     3001 ggcgcaagta cgatcccgag gcgaagatgc gcccggtggc gacggtgtaa ctagaccatt
     3061 gttgattctt gatttgggat tgttgattcc ttgttgattg gagattcttg attttcgatc
     3121 tatttgcgat tgcctgattg tcgattcgcc atggctgttt cgcgggaggg ccaccgggcc
     3181 ttgttgaacc tacagcaagg gacgaacgca tgcacactcg aatcgcttcg cgttcacgag
     3241 ggcgcggacg tcaagctcga ccgtgtgcga ttctggcctt cctgatcgtc gtttgctccg
     3301 cgccgctcgc agggcagtct tccgctaccc gactgaccgc cccagcgttc gaggtggcgt
     3361 cagtcaagcc ctcgaaggac gaccgtccac cgttcttcag cacccgaccg caaggaacag
     3421 ttgtgatcac cggccaggaa ctgagaacga ttgtcgcctt ggcgtacggc atcgacctca
     3481 gggtcgcgcg tttcgtgctc cgcggcggac cggagaacat cctgtccgcc cgattcgaca
     3541 tcacaggcaa gccgccggac gacggtccgc cggggcagca tctgttgatg ttgagaacgc
     3601 tcctggcaga gcggttccgc ctgcgcagcc ataccgagac gcgacagatg ccgatctacg
     3661 cgctcaccgt tgcacggaag ggaacgttgg ggccgtcgct tcagctctcc agccacgact
     3721 gcgtcgcttt cgtcgcggca ggtggcaaga gaaccgacgc cgacgcgccc gtcgattcga
     3781 gtaaccggcc actgtgttgg ggcacgtacg gtttcgcaga agcgggacca gggacagaat
     3841 caatgaggta cgcgggaccg ctcgcggccc tcgttgagcg cctccagccg atgctcgatc
     3901 gcccggtcgt cgatatgaca ggactgaagg gaagctacga gtggcgcctc actttcgctc
     3961 gcgatccgag aaccgactcc gagttccccc caatcttcac tgccgttgac gagcagctcg
     4021 ggctgagcct ggtggcccgc acgggtccgg tcgaagtact cgtcatcgat tcggtcgatc
     4081 cgccagtacc tgattgagcg ggagaatcac caatcatcac caatcgacca tcaccaacga
     4141 atcaacaatc aaaga
//
LOCUS       DBNGSH010001063         1513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_508211, whole genome shotgun sequence.
ACCESSION   DBNGSH010001063 DBNGSH010000000
VERSION     DBNGSH010001063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_508211"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1513
                     /locus_tag="AC16CY_24035"
     CDS             18..>1513
                     /locus_tag="AC16CY_24035"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR3356944.1"
                     /translation="MPELDRRRAIGSLLKFAVLAPFAEEIAAAGSAAIALPALQAQPL
                     LSQVRRLVDAMEYLGEPFSAAERERLDTAANQNDRARAIEDVQQVLDPRCLLLVRINP
                     ESRVSVERGAAPARLVEHGWRAYLIKVRNEAAVTGVLNVESPQAQPVYRPSTGSPAPT
                     ESVRQADVTDRWLALDGYTQKPMEAPLSGLELEYRIALFYSRDPGRREAQLGAVVGPV
                     TADVGFRNRTAVLFDIAPSRDVTIRVRDENGRPVMASFRITDKAGRVYPARSKRLAPD
                     FFFHEQVYRADGETIRLPAGEFTVTCGRGPEYIPETRTVRLSSSASSLDFTLRRWIDP
                     PSRGWYSGDHHIHAAGCSHYESPTEGVRPEDMMRHVLGEALSVGSVLNWGPSYYHQRQ
                     YFEARDNKVSTSSSLIRYDLEVSGFPSSHCGHIVLLRLENQDYPGATKIEEWPTWDLP
                     ILKWAKAQGAVVGFAHSGFGLQLPSPDLPNYQMPPFNSIGANEYIVDVA"
BASE COUNT          246 a          546 c          487 g          234 t
ORIGIN      
        1 agttcacgga ggtcgtcgtg cccgaactcg atcgccgccg cgctatcggc tccctgttga
       61 agttcgcggt cctggccccg ttcgccgagg agatcgccgc ggcgggctcg gccgccatcg
      121 cgctgcccgc ccttcaggcg cagccgctcc tctcccaggt cagacgcctc gtcgacgcga
      181 tggagtacct tggcgagccg ttcagcgccg cggagcgcga acggctcgac acggccgcca
      241 atcagaacga ccgcgcccgc gcgatcgagg acgtccagca ggtcctcgac ccgcgctgcc
      301 tcctgctcgt ccgcatcaat cctgaaagcc gggtctcggt cgaacgcggc gcggcgccgg
      361 cgcggctcgt cgagcacgga tggcgggcct atctgatcaa agtgcggaac gaagccgcgg
      421 tcaccggcgt gctcaacgtg gagagccccc aggcgcagcc ggtctaccgg ccgtcaaccg
      481 gatcgcccgc tccgacggag tctgtccgac aagcggatgt cactgaccga tggcttgccc
      541 tggacggcta cacgcagaaa ccgatggagg cgccgctctc cggactggag ctcgagtacc
      601 ggatcgcgct cttctacagc cgggaccccg gacgccgcga ggcgcagctc ggagcggtgg
      661 tcggcccggt caccgcggat gtcggattcc ggaaccggac ggcggtgctc ttcgacatcg
      721 cgccatcccg cgacgtcacg atccgggtcc gcgacgagaa cggacgcccc gtcatggcgt
      781 cgttccgcat cacggacaaa gccgggcggg tgtatccggc acgatccaag cgcctggcgc
      841 cagacttctt cttccacgag caggtgtatc gcgccgatgg cgagaccata cggcttcccg
      901 ccggagagtt caccgtgacc tgcgggcgcg ggcccgagta catcccggag acacgcacgg
      961 tccgcctgag ctcgtccgcc tcgtcactcg acttcacgct ccggcgctgg atcgatccgc
     1021 cctcgcgcgg ctggtattcg ggagaccatc acatccacgc cgccggctgc tcgcactacg
     1081 agagcccgac cgagggcgtg cggccggaag acatgatgcg ccacgtgctg ggcgaagcgc
     1141 tcagcgtcgg ttcggtcctg aactggggcc cgagctacta ccaccagcgg cagtacttcg
     1201 aggcccggga caacaaggtc tccacctcat cgtccttgat ccgctacgac ctcgaagtgt
     1261 cgggattccc ctccagtcac tgcggccaca tcgtcctcct gcggctcgag aaccaggact
     1321 acccgggcgc cacgaagatc gaggagtggc ccacgtggga ccttccgatc ctcaagtggg
     1381 cgaaggccca gggcgccgtg gtcggattcg cgcactcggg cttcggcctg cagcttccgt
     1441 cgccggatct gccgaactac cagatgccgc cgttcaactc gatcggcgcc aacgagtaca
     1501 tcgtggacgt ggc
//
LOCUS       DBNGSH010001064         6543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_848695, whole genome shotgun sequence.
ACCESSION   DBNGSH010001064 DBNGSH010000000
VERSION     DBNGSH010001064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_848695"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="AC16CY_24040"
     CDS             <1..242
                     /locus_tag="AC16CY_24040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356945.1"
                     /translation="PDFVFTVTRDFVRNCQTPVLILPDDIPSHPYAVAMEAAMLAPKA
                     EVSMFPWKEPKERIPLAVRQIRSFLRAHRPVDRRV"
     gene            complement(294..719)
                     /locus_tag="AC16CY_24045"
     CDS             complement(294..719)
                     /locus_tag="AC16CY_24045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356946.1"
                     /translation="MTGLFLRRFAVMLLVLLTVACSGDPENPAGGPSGTGVGSDPQIA
                     NNTDDFQWQLSGANNFSTGGGYSWRNTGTAARVNISNSISSGSGQVLVMDASGRTVWQ
                     GNLTNNGTFNSEPGPAGDWRVEVLPSGVSGSMNIRVQKN"
     gene            complement(923..1861)
                     /locus_tag="AC16CY_24050"
     CDS             complement(923..1861)
                     /locus_tag="AC16CY_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HIR3356947.1"
                     /translation="MRRISIVLLTLICFTTIASAQNPVVVTVTGAPGGSGLVVSLVSA
                     DDIVARLMSFDSDRDGKVTVSELPERMQALVARGDVSGDGTLDDAEIAKLAKSPAQDA
                     RAFFTSGGYTFGDQVGLSSRAHVEGAIEDLRLPSATKEQALDAARWFMDGLETDAGAD
                     LLSELKPLLTTEQITAFKAALAKGGTAGGLLVSVSLPQNGEPRNFVVQSGMELQVARL
                     LLPPAQRLKAMAAVERFKARLRPGNEERAALMEAMKGILSDEERDNLRAALERRPLVK
                     SRAPLAFKDVVTVGGVVDLRREMIAAPAPQAPEARR"
     gene            complement(1891..3003)
                     /locus_tag="AC16CY_24055"
     CDS             complement(1891..3003)
                     /locus_tag="AC16CY_24055"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3356948.1"
                     /translation="MNVCLPLATAGFVFLSASALALASAEGERTRTLRLPEVSYRYAE
                     LNLPAHFRQSQAQRFDNTPAENPVTDQGATLGRVLFYDTRLSANDTISCGSCHVQAHG
                     FADPNRFSRGFKGGLTDRHAMNLVNLRFHVKGRFFWDERGGNLESMVLLPVENRLEMG
                     LDLERLPGILSRDPRYAELFGRAFGDAEVTNERIARALAQFLRSMVSYQSKYDEGRAR
                     ANKAAEDFVNFTVQENRGKALFLRNCSLCHLPDQEAHFVMTEPVNTGLDDDTAFSDGG
                     VGDITLNPRDVGHFKSPSLRNVEVAGPYMHDGRFETLEAVIDHYSSGGKKHPNRDVRI
                     QPLNFTASEKAALIAFLQTLTDRKFLEDPKFSDPFE"
     gene            complement(3062..3811)
                     /locus_tag="AC16CY_24060"
     CDS             complement(3062..3811)
                     /locus_tag="AC16CY_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIR3356949.1"
                     /translation="MREMLAEFLGTFILIVFGTAVVAQVVLSGQANGSYLSINLGWGL
                     AVVMGVYVAGGVSGAHLNPAVTLTLAVRRGFPWGKVLPYCAAQLTGAFAGALVTFIVY
                     REAFAHFDGGVRQVMGPQGTAGIFATYPQAFLSTFPGGLIDQIVGTALLLILIFAIGD
                     AKNLAPDPKAAPIVVGLAVTLIGMTFGYNAGYAINPARDLGPRIFTAVAGWGGEVFRA
                     GNGWWWVPIAGPLIGGVLGGYVYDALVGKRR"
     gene            3810..5300
                     /gene="glpK"
                     /locus_tag="AC16CY_24065"
     CDS             3810..5300
                     /gene="glpK"
                     /locus_tag="AC16CY_24065"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR3356950.1"
                     /translation="MSYILALDQGTTSSRAILFDREGAIKGVAQREFRQIFPQPGWVE
                     HDAMEIWTSQAGVAVEALAGAGLRARDVSAIGITNQRETTVVWDRKTGQPITNAIVWQ
                     DRRTADDCDRLRSEGREPLFRERTGLVLDAYFSGTKLAWILDNVDGARKRAEAGELAF
                     GTIDSWLVWKLTDGRLHITDATNASRTLLFNIHTLQWDDELLRALRVPRGLLPEVRNS
                     SEVYGEVTTTLGLESVPIAGIAGDQQAALFGQMCTSPGLTKNTYGTGCFMLQNTGGKA
                     PVSQHKLVTTVAWMRGGKAEYALEGSVFIGGAVVQWLRDGLGIIRSSSDVEALALSVP
                     DNGDVYLVPAFAGLGAPHWDQYARGAIVGLTRGTTAGHIARAALESIAYQSADLLDAM
                     QADAGTKLAELRVDGGAARNDLMMQFQSDLIGAPVVRPKITETTALGAAYHAGLAVGF
                     WKSGEDIASQWRSERRFEPKMPASRVDALRGRWRQALERAKGWIER"
     gene            5297..>6543
                     /locus_tag="AC16CY_24070"
     CDS             5297..>6543
                     /locus_tag="AC16CY_24070"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR3356951.1"
                     /translation="MNRAEMLDRAMQRREPWDMVIVGGGAVGMGVAVDAASRGYEVLL
                     VEQHDFGKGTSSRSTKLVHGGVRYLEQGAVSLVMEALKERGLLRRNAPHLVTDLAFVV
                     PNYEWWEAPFYGVGLKVYDLLAGRYGFGAAEILSREETLARLPTIKTDGLRGGVVYYD
                     GQFDDTRLLINLAATAVEQGAVALNYTPATAVTRGADGFADGIVARDVEGGHEFRAPA
                     RVVVNAAGPQSDRVRRLAHPDAAPLIAPSQGIHLVFDGSLLLGGHAIMVPHTTDGRVM
                     FAIPWHGHTVVGTTDTPVDEVPLEPRPLDEEIDFILETASHYLQRPVSRADVLSVFAG
                     IRPLVRSGSGGATASTTASLSRDHTIHIDPSGLLTIAGGKWTTYRHMAEDCVDHAATL
                     AHLPEKPCVTRNLNVHGFHHQAH"
BASE COUNT         1093 a         2187 c         2221 g         1042 t
ORIGIN      
        1 accccgattt cgtcttcacg gtcacacgcg acttcgtgcg caactgccag acgccggtgc
       61 tgatcctgcc ggacgacatc ccgtcgcatc cgtacgccgt ggcgatggag gccgccatgc
      121 tggcgccaaa ggcggaagtg agcatgttcc cgtggaagga accgaaggag cggattcccc
      181 tggcggtgcg ccagatccgc tcctttctcc gggcacatcg gcctgttgat cggcgcgtct
      241 gaagacgcgc cgctaccagt agaggcgggt ctttagaccc gccttgccca tggtcagttt
      301 ttctggacgc ggatattcat gctgccggac accccggacg gaaggacttc aaccctccag
      361 tcaccggcgg gtccgggttc cgagttgaac gtgccgttgt tcgtgaggtt tccctgccag
      421 acggtgcgcc ccgaggcgtc catgacgagc acctgcccgc tgccggacga aatggagttc
      481 gagatattga ctctggccgc cgtgcctgtg ttccgccacg agtaaccgcc gccggtcgag
      541 aaattgttgg cgccactcaa ctgccattgg aagtcgtccg tgttgttggc aatttgcgga
      601 tcgctgccga caccggtgcc actcggacca cccgctggat tttcggggtc gccgctgcat
      661 gccacggtca acagcacgag cagcattacg gcgaatcgcc gaaggaacag acctgtcatg
      721 atgtgacccc cctccatatc cgcaaatagc aagaaagggg ccgcgtgaag cgcgcgcgtg
      781 ctcagccgtt tttcgcccga aagaaccgca gacgcgaagc acggggcact ccgcgagtct
      841 cggcgtcgcg tcaaacgtgc cgtggacgtc agtgcattga cagttctggg cgtcgtgaaa
      901 aacggcgcag aatcgcgtgt cttcagcgcc tggcctcggg cgcttgcggt gcgggagcgg
      961 cgatcatttc cctgcggagg tcgacgacgc ccccgaccgt cacgacgtct ttgaaagcga
     1021 ggggcgcgcg tgatttgacc aggggtctgc gctcgagcgc cgcgcggagg ttgtcgcgct
     1081 cttcgtcgct gagaatgccc ttcatcgcct ccatgagcgc ggcccgctcc tcattcccgg
     1141 ggcggagccg ggctttgaaa cgctcgacgg ccgccatcgc cttgagccgc tgcgcgggtg
     1201 gcagcaaaag ccgcgcgacc tgaagttcca taccggactg cacgacgaag ttgcggggct
     1261 cgccgttctg gggcagcgag acggaaacga gaaggccacc ggcagtgccg cctttcgcga
     1321 gcgccgcctt gaaagcggtg atctgctccg tcgtgagcaa gggcttcagc tcgctgagaa
     1381 ggtccgcccc ggcgtctgtt tcgagcccgt ccatgaacca tcgggcggcg tcgagcgcct
     1441 gctctttcgt cgcgctgggg agcctgaggt cctcgatcgc cccttcgacg tgcgcgcgcg
     1501 acgacagccc gacctgatcg ccgaaggtat agccgccgga cgtgaagaac gcgcgcgcgt
     1561 cctgggctgg cgacttggcg agcttggcta tttcggcgtc gtcgagcgtg ccgtcgccgc
     1621 tgacatcgcc ccgggccacc agggcctgca tccgctcggg gagctcgctc accgtcacct
     1681 tcccgtcgcg gtcgctgtcg aaggacatca ggcgagcgac gatgtcatcg gcgctcacga
     1741 gcgagacgac gagcccggat ccgcccggcg ctcctgtcac ggtcaccacc acggggttct
     1801 gcgccgacgc gatcgtcgtg aagcaaatca gcgtcagcag gacgatggag atgcgccgca
     1861 tacgtgtgct ccttcgagtc cttggactcg ctactcgaac ggatccgaga acttcggatc
     1921 ctcgaggaat ttccgatcgg tcaacgtctg cagaaaggcg atgagcgccg ccttctcgga
     1981 ggcggtgaag ttcagcggct ggatgcgcac gtcccggttc gggtgcttct tcccgccgct
     2041 gctgtagtgg tcgatgacgg cctcgagcgt ctcgaagcgt ccgtcgtgca tgtacggccc
     2101 cgcaacctcg acgttgcgga gtgacggcga cttgaagtgc ccgacgtcgc gcgggttgag
     2161 cgtgatgtcg ccgacgccgc cgtcgctgaa cgccgtatcg tcgtccagcc cggtgttcac
     2221 cggctcggtc atgacgaagt gggcctcctg gtccgggagg tggcacagcg aacagttccg
     2281 gagaaagagc gccttgcccc ggttttcctg caccgtgaag ttcacgaaat cctccgcggc
     2341 cttgtttgcc cgcgcccgcc cctcgtcgta cttcgactga taggacacca tcgagcgcag
     2401 gaactgcgcc agcgcacgcg cgatgcgctc gttggtgacc tcggcgtcac cgaatgcccg
     2461 accgaagagc tcggcgtacc gcgggtctcg cgagaggatc cctggcaggc gttcgaggtc
     2521 cagccccatc tcgagccggt tctcgaccgg cagcagcacc atcgattcga gattgccgcc
     2581 gcgctcgtcc cagaagaagc ggcccttgac gtggaatcgc aggttgacga ggttcatggc
     2641 gtgccggtcg gtcagccctc ccttgaaccc ccggctgaag cggttgggat cggcgaaccc
     2701 gtgtgcctgc acgtggcagg aaccgcacga gatggtgtcg ttggccgaga gcctggtgtc
     2761 gtagaacagc acgcggccaa gcgtcgcccc ctggtccgtg actggattct ccgcgggcgt
     2821 gttgtcgaag cgctgcgcct gtgactgcct gaaatgggcc ggtaggttga gctcggcgta
     2881 acgatacgaa acctctggca gccgaagcgt ccgtgtccgt tcgccctccg cgctcgcgag
     2941 tgccagcgcc gaggccgaca gaaagacgaa gcccgccgtc gccaagggga ggcagacgtt
     3001 cattcgccct actagccggt ttagacaccg caccggccga attggccttg ggggttggga
     3061 gttacctccg cttccccacc agcgcgtcgt acacgtaccc cccgagcacg ccgccgatga
     3121 gcggccccgc aatcggaacc caccaccagc cgttgcccgc gcgaaagacc tcgccgcccc
     3181 accccgccac tgccgtgaag atccgcggac cgaggtcgcg cgccgggttg atcgcatacc
     3241 cggcgttgta gccgaaggtc atgccgatga gggtcaccgc cagcccgacc acgatgggag
     3301 cggccttcgg gtcgggggca aggttcttcg cgtcgccgat cgcgaagatg aggatcagca
     3361 gcagcgcggt gccgacgatc tgatcgatca gacctccggg aaacgtgctg aggaacgcct
     3421 gcggataggt ggcgaagata ccggccgtcc cctgcggccc catgacctgt cgtacgccgc
     3481 cgtcgaagtg ggcgaacgcc tcgcgataga cgatgaacgt caccagcgct ccggcgaacg
     3541 cgccggtaag ttgcgccgcg cagtacggaa gcactttccc ccacggaaag ccgcgccgga
     3601 ccgcgagcgt cagcgtcacg gccggattga ggtgcgcgcc cgatacgccc cccgcgacgt
     3661 acacgcccat gacgacggcc aggccccagc cgagattgat cgacaggtac gacccgttgg
     3721 cctgccccga cagcacgacc tgcgccacaa cggccgtccc gaacacaatc agaatgaacg
     3781 tgccgaggaa ctcggctagc atctcacgca tgtcgtacat cctcgccctg gaccagggca
     3841 cgacgagctc gcgcgctatc ctgttcgatc gggaaggcgc aatcaaggga gtcgcgcaac
     3901 gcgagttccg gcagatcttt ccgcagccag gctgggtcga gcacgacgcg atggagatct
     3961 ggacgtcgca ggcgggcgtc gccgtggaag cgctggccgg cgccggcctc cgtgcccgcg
     4021 acgtgtccgc catcggcatc accaaccagc gcgagacgac cgtcgtctgg gatcgcaaga
     4081 ccggccaacc catcaccaac gccatcgtct ggcaggatcg gcgcacggcg gacgactgcg
     4141 atcggctgcg atcggagggc agggaaccgc tgtttcgcga gcgtacgggc ctggtgctcg
     4201 acgcgtactt ctcgggcacg aagctcgcct ggattctcga caacgtggac ggggcgcgga
     4261 agcgcgcgga ggcgggcgag ctggcgttcg gcacgatcga ctcgtggctc gtctggaagc
     4321 tcaccgacgg ccgactgcac atcaccgacg ccacgaacgc gtcgcgaacg ctgctgttca
     4381 acatccacac gcttcaatgg gacgacgagt tgctgcgcgc cctgcgggtg ccgcgtggcc
     4441 tgctgccgga ggtacgcaac tcgagcgagg tgtacggcga ggtgacgacg acactcggcc
     4501 tggagtcggt cccgatcgcg gggatcgccg gcgaccagca ggcggcgctg ttcgggcaga
     4561 tgtgcacctc gcctggcctg acgaagaaca cctacggcac cggctgcttc atgctgcaga
     4621 acaccggggg caaggcgcct gtctcccagc acaagctggt gacgacggtc gcctggatgc
     4681 gcggcggcaa ggcggagtat gcgctcgagg ggagcgtgtt catcggcggc gcggtcgtgc
     4741 agtggcttcg cgacggcctc ggcatcattc gatcgtcgag cgacgtcgag gcgctggcgc
     4801 tgtcggtgcc cgacaacggc gacgtctacc tcgtgcccgc ctttgccggg ctcggcgcgc
     4861 cccactggga tcagtacgcg cggggcgcga tcgtcgggct gacccgcggc acgaccgccg
     4921 gccacatcgc gcgcgcggcg ctcgaatcga tcgcctatca gtcggcggac ctgctggatg
     4981 ccatgcaggc cgacgcgggc acgaagctgg ccgagcttcg cgtggacggc ggcgcggcgc
     5041 ggaacgatct gatgatgcag ttccagtcgg acctgattgg ggcgccggtg gtgcgcccga
     5101 agatcaccga gacgacggcc ctgggcgcgg cgtaccacgc gggcctcgcg gtcgggttct
     5161 ggaagagcgg agaggacatc gccagccagt ggcggagcga gcgccgcttc gagccaaaga
     5221 tgccggcctc gcgcgtcgat gcgctgcgcg ggcgctggcg gcaggcgctc gagcgcgcca
     5281 aggggtggat cgagagatga accgcgccga gatgctcgac cgcgccatgc agcggcgaga
     5341 gccgtgggac atggtcatcg tcggcggagg cgccgtcggc atgggcgtcg ccgtggacgc
     5401 ggcctcgcgc ggctacgagg tgctgctcgt cgagcagcac gacttcggca aaggcacgtc
     5461 cagccgatcg acgaagctgg tgcacggcgg agtccggtac ctcgagcagg gcgccgtgtc
     5521 gctggtgatg gaggcgctga aggagcgcgg cctgctccgg cggaacgcgc cgcacctggt
     5581 caccgacctg gcgttcgtcg tcccgaacta cgagtggtgg gaggcgccct tctacggcgt
     5641 cggcctgaag gtgtacgacc tgctggcggg acgctacggc ttcggcgccg ccgagatcct
     5701 gtccagggag gaaacactcg cgcgcctgcc gacgatcaag accgacgggc tgcgcggcgg
     5761 ggtcgtctac tacgacgggc agttcgacga cacgcggctc ttgatcaacc tggcagccac
     5821 cgcggtggaa cagggggccg tcgcgctgaa ctacacgccc gccacggcgg tgacgcgcgg
     5881 agccgacggc ttcgccgacg gcatcgtcgc gcgcgacgtg gagggagggc acgagttccg
     5941 cgcgcccgcg cgtgttgtcg tcaacgccgc cgggccccag agcgatcgcg tgcggcggct
     6001 cgcccatccg gatgccgcac cgctcatcgc tcccagccag ggcatccatc tcgtgttcga
     6061 cggatcgctg cttctgggcg ggcacgcgat catggtgccg cacacgacgg acggacgcgt
     6121 gatgttcgcg atcccgtggc acggccatac cgtcgtcggc acgaccgaca cgccggttga
     6181 cgaggtcccg ctcgagccgc gtcccctgga cgaggagatc gacttcatcc tggagaccgc
     6241 gagccactac ctgcagcggc cggtctcgcg cgccgacgtg ctgagcgtgt tcgcgggcat
     6301 caggccgctg gtgcgatcgg gatcgggcgg cgcgacggct tccacgaccg cgtcgctctc
     6361 gcgcgaccac acgatccaca tcgacccgtc aggcctgctc acgatcgcgg gaggaaaatg
     6421 gacgacctac cgccacatgg cggaggactg cgtggaccat gccgcgacgc tcgcgcacct
     6481 gcccgagaag ccgtgcgtga cgcggaacct caacgtgcac ggcttccatc atcaggcgca
     6541 caa
//
LOCUS       DBNGSH010001065         3061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1527422, whole genome shotgun sequence.
ACCESSION   DBNGSH010001065 DBNGSH010000000
VERSION     DBNGSH010001065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1527422"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="AC16CY_24075"
     CDS             <1..1936
                     /locus_tag="AC16CY_24075"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3356952.1"
                     /translation="YVGSLRAMTDPLSLGLTVVDARAQGMGAARGATDFGQYFVYFSF
                     FIVVSALVLAVLFFKLGIEQRVREVGLLRAVGFGPAQVRRLFLQEGLLLAVVGSALGV
                     AGALGYASLMMFGLRTWWVDAVGTTALRLHVSSASLAAGALGGIVAAVACIWWTLRSL
                     NSVSERSLLMGEIEGDHGPAKAGHYVRRWRLPLAAVGLFVLAVVLMVASAVGAINPAG
                     GFFGAGSAILIAWLCGSLHLFRRRSRQSIAGQGWLQVSRLGLRNAGYRPGRSVLSIAV
                     VASAVFILISVDAFRRGEQLADSGPRSGVGGYSLIVETLLPIVHDPNSQAGKDAMNLV
                     GLDESVTFEPFRLLPGDDASCLNLYEPRNPRIMAPSDAFVKKGRFAFQSSLASTDAER
                     ANPWLLLDRAEPDGAVPVIADANSMTYVLHKSLGEEIELPRGDRTIRLRLVAALRDSI
                     FQGELLMAQSQFLRLFPEQDGYRVLLVSTKPDRVSEVKASLDDGLADFGPTTSGTAER
                     LAQFHRVENTYLSTFQTLGGFGLLLGTLGMAAVLLRNVLERRRELALLGAVGYRRTHV
                     LMMVVAETALLLGGGLLAGATSAALAIAPAVAERGGRFPLTRAGALLVFVVFVTGLLS
                     SVLAMRVGTRAPLLTALRSE"
     gene            1941..>3061
                     /locus_tag="AC16CY_24080"
     CDS             1941..>3061
                     /locus_tag="AC16CY_24080"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3356953.1"
                     /translation="MTSRVGSVVCIAILASSSVVFAENWPQWRGPSLNGISAEKNLPV
                     RWSATENVVWKLAVPERSGSTPIVWGDHVFLNVGEGASLALWAVNRSNGTVRWKAALG
                     DGNRRMQKQQMSSPSPVTDGRSVWVMTGTGVLKAFDFSGKQLWARNIQDDYGRFGLQW
                     GYASSPLLHEDSLFVQVLHGMRTDDPSYLLRIDKATGKTLWRVERPTNARFESPDAYT
                     TPALLRHAGSTEIVITGGDVVTGHDHATGRELWRADGLNPRNDGSYRIVASTVVHGDL
                     IFAPSRERPLLALKPGGRGDVTRSHVLWSFNNGPDVPTPVTDGTYLYSINDRGIMYCL
                     DAKTGKTVYGPQRLRNATYSGSAVLADGKIYITDEDGVTT"
BASE COUNT          467 a          951 c         1070 g          573 t
ORIGIN      
        1 gtacgtcggc agcctgcgcg ccatgaccga tccgctgtct ctggggttga ccgtcgttga
       61 cgcacgcgcg caggggatgg gcgccgcccg cggcgcgacg gacttcggcc agtatttcgt
      121 ctacttcagc ttcttcatcg tcgtctcggc gctggtgctg gcggtgttgt tcttcaagct
      181 ggggatcgag cagcgcgtgc gggaggtcgg tctcctacga gccgtcggtt tcgggcctgc
      241 gcaagtgcgg cggctgtttc tccaggaggg tctgctgctg gctgtcgtgg gcagcgccct
      301 gggcgtcgcc ggcgcgttgg gctacgcctc cctcatgatg tttggcctgc gaacgtggtg
      361 ggtcgacgca gttggcacga cggccctgag gctgcacgtc tcgagcgcat cgctggcggc
      421 cggagcgctt ggcgggatcg tcgcggcagt cgcctgcatc tggtggacgc tccgctccct
      481 caacagcgtc tccgagcgca gcctgctgat gggcgagatc gagggggatc acggtccggc
      541 taaagccgga cactacgtac ggcgatggag attgccgctc gcggctgttg gtctcttcgt
      601 gctcgccgtt gtgctgatgg tggcgtccgc agtcggcgcg atcaatcctg cgggaggatt
      661 cttcggcgca ggctcggcca tcctcatcgc gtggctgtgt ggatcgctcc acctgttcag
      721 gcggcgctcg cggcagtcga tcgccgggca gggctggctg caggtgtcgc ggctcgggct
      781 gcgcaatgcc ggctaccggc cgggccgcag cgtgctctcg attgccgtgg tcgcgtcggc
      841 cgtcttcatt ctgatctcgg tcgatgcgtt tcgacggggc gaacagctgg ccgactctgg
      901 tccccgatcc ggcgtcggcg gctacagcct gatcgtcgaa acactcctgc ccatcgtgca
      961 cgatcccaat tcgcaggccg ggaaggacgc gatgaatctc gtcgggctcg acgagtcggt
     1021 gacgttcgag cccttcaggc tgcttccggg cgacgatgcc agctgcctga acctgtacga
     1081 gccaagaaac cctcgaatca tggcgccttc cgatgcgttc gtgaagaagg ggcggttcgc
     1141 ctttcagagc tcgctcgcgt ccaccgacgc cgagagagcc aacccatggc tgctgctgga
     1201 tcgcgccgag ccggatggtg ccgtcccggt gatcgcggac gcgaactcga tgacgtacgt
     1261 gctgcacaag agtctgggcg aggagatcga gctgcctcgc ggagatcgca cgatcaggct
     1321 caggctcgtg gccgccctgc gcgacagcat cttccagggc gagctgctga tggcgcagag
     1381 ccagttcctc aggttgtttc ccgaacagga tggttaccgc gtactgctcg tcagtacgaa
     1441 gcccgatcgc gtgtccgaag tgaaggcatc gctcgatgac gggctcgccg acttcgggcc
     1501 gaccacgagc ggtacggcgg aacggctggc ccagttccac cgcgtcgaga acacgtactt
     1561 gtcaacattt cagacgctcg ggggattcgg cctcctcctt ggcacgcttg gcatggccgc
     1621 ggttctgctt cgcaacgtgc tggagcggcg gcgcgagctg gcgctgcttg gcgcggtcgg
     1681 ctatcgacgg acgcacgtgc tgatgatggt cgttgccgag acggccctgc tgcttggagg
     1741 cggcctgctc gcgggagcaa ccagcgcggc gctggcgatc gcaccagccg tggccgagcg
     1801 cggcgggcgt tttccactga cgagagctgg cgctcttctg gtgttcgttg tcttcgtaac
     1861 cggtctgtta tcctcggtcc tggcgatgcg agtgggaacc agggcgccgc ttctcacggc
     1921 gctccgatcg gagtagctca atgacgagtc gagtgggttc ggtggtgtgt atcgcgattc
     1981 tggcgtcatc atctgtcgtg ttcgcggaga actggcccca gtggaggggc ccatcgctca
     2041 acgggatcag cgcggaaaag aacctgcctg tgcgctggtc cgcgaccgag aacgtggtgt
     2101 ggaagctcgc agtgccggag cgatcgggat cgacaccgat cgtgtggggc gaccacgtgt
     2161 ttctcaacgt gggggaggga gcgagcctgg ccctgtgggc ggtcaatcgg tcgaacggaa
     2221 cagtcaggtg gaaggccgct ctgggagacg gcaaccggcg gatgcagaag cagcagatgt
     2281 cgtcgccctc gccggtcact gacggccggt ccgtgtgggt gatgacgggt acgggcgtgc
     2341 tcaaggcgtt cgacttctcc ggcaagcagc tctgggcgcg caacatccag gacgactacg
     2401 gacggttcgg cctccaatgg ggctacgcct cgtcccctct gctccacgag gattcgctgt
     2461 tcgtgcaggt actgcacggc atgaggaccg acgatccgtc gtatcttctg aggatcgaca
     2521 aggcgaccgg gaagactctg tggcgcgtgg agcggcccac gaacgcgcgg ttcgagtctc
     2581 ccgacgctta cacgacgccc gcgctgctgc gtcacgccgg ctccacggag attgtcatca
     2641 cgggcggcga cgtggtgacg ggtcacgacc acgcaacggg gcgagagctc tggcgtgcag
     2701 acggcttgaa cccacgcaac gacggcagct accgcatcgt cgcatcgacg gtcgtgcacg
     2761 gggatctgat ctttgcgcct tcgcgcgagc gcccgctgct ggcgctgaag cctggcggcc
     2821 gcggcgacgt cacgagatcg cacgtcctgt ggtcgttcaa taacgggccc gacgtgccga
     2881 ccccggtgac cgacggcacc tacctgtatt cgatcaacga ccgcggcatc atgtactgcc
     2941 tcgacgccaa gacggggaag acggtgtacg gaccgcagcg gctgcggaat gccacctaca
     3001 gcggctcggc cgtgctggcc gacgggaaga tctacatcac cgacgaggac ggcgtgacca
     3061 c
//
LOCUS       DBNGSH010001066         1514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_3734828, whole genome shotgun sequence.
ACCESSION   DBNGSH010001066 DBNGSH010000000
VERSION     DBNGSH010001066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3734828"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..>1514)
                     /gene="pruA"
                     /locus_tag="AC16CY_24085"
     CDS             complement(8..>1514)
                     /gene="pruA"
                     /locus_tag="AC16CY_24085"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - L-glutamate gamma-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - L-proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HIR3356954.1"
                     /translation="IEIPIVIDGCAIRTGRVEPAVMPHDHRHVLGDWHAADPSHVQKA
                     IDAAARARREWASWPLQDRAAVFLKAAELLTTTWRATLNAATMLGQSKTVIQAEIDSA
                     SELIDFWRFNAHFAQELYAEQPISSHTMWNSVEYRPLEGFVYAVTPFNFTAIGGNLPT
                     APALMGNTVVWKPASSAMLSAHYILKLLEASGLPPGVINMVPGNAAEITAALLDSPDL
                     AGIHFTGSTAVFNSMWKRVGENAGRYRGYPRLVGETGGKDFIIAHSSADVQELAVAIV
                     RGSFEYQGQKCSAASRIYVPRSLWGEVKDRVVAMMRDIRMGDVADFRTFMGAVIDRKA
                     FTKISGYLDDARRNATIVQGGGARDDVGYFIEPTLVEASDPGYRLLCEEIFGPVVTAY
                     AYPDAQWEQTLDVVDRTSPYALTGAVFARDRRAVLQASSALRNAAGNFYINDKPTGAV
                     VGQQPFGGARASGTNDKAGSKMNLLRWVSARAVKETFSPPREYLYPYMTEQ"
BASE COUNT          229 a          528 c          494 g          263 t
ORIGIN      
        1 gacctcttca ttgctccgtc atgtaggggt acaagtactc ccgcggcggc gagaacgtct
       61 ccttcacggc ccgcgcgctc acccagcgga gcagattcat cttcgagccc gccttgtcgt
      121 tggtgcccga cgcgcgcgcg ccgccaaacg gctgctgccc cacgacggct cccgtgggct
      181 tgtcgttgat gtagaagttg ccggccgcgt tgcgcagcgc gctcgacgcc tggaggacgg
      241 ctctgcgatc gcgcgcgaac accgcgcccg tcagggcgta gggcgaagtc cgatcgacga
      301 cgtcgagcgt ctgttcccac tgcgcgtccg ggtacgcata ggccgtgact accgggccga
      361 agatctcctc gcacagcagt cgatagcccg ggtcggacgc ctcgaccagc gtcggctcga
      421 tgaaataccc gacgtcgtcc cgcgcgcccc cgccctggac gatcgtggcg ttcctccgcg
      481 cgtcgtccag gtagccgctg atcttcgtga acgccttgcg gtcgatcacc gcgcccatga
      541 acgtgcggaa gtccgcgacg tcgcccatgc ggatgtcgcg catcatcgcg acaacgcggt
      601 ccttgacctc cccccacagc gagcggggca cgtaaatccg gctggccgcc gagcacttct
      661 gtccctggta ctcgaagctg ccgcggacga tcgccactgc cagctcctga acgtcggcgg
      721 aagagtgcgc gatgatgaag tccttgccgc ccgtctcgcc gacgagcctg ggatagccgc
      781 ggtagcgccc cgcgttctcg ccgacgcgtt tccacatcga gttgaagacg gcggtgcttc
      841 cggtgaaatg aatcccggcg agatcgggtg aatcgagcag cgccgccgtg atctcggccg
      901 cgttgcccgg caccatgttg atcacgcccg gcggcaggcc cgacgcttcg agcagcttga
      961 ggatgtaatg cgcgctcagc atcgcgctcg atgccggctt ccagacgacc gtgttgccca
     1021 tgagcgcggg cgccgtcgga aggttcccgc ctatcgcggt gaagttgaag ggcgtcaccg
     1081 cgtacacgaa tccctcgagc ggccggtact cgacgctgtt ccacatcgta tggctgctga
     1141 tcggctgctc cgcgtagagc tcctgcgcga agtgcgcgtt gaaccgccag aagtcgatga
     1201 gctccgaggc cgaatcgatc tccgcctgga tcaccgtctt cgactgcccc agcatggttg
     1261 ccgcgttcag cgtggcccgc cacgtcgtcg tcagcagctc ggcggccttg aggaagacgg
     1321 ccgcgcggtc ctgcagcggc cagctcgccc actcgcgcct cgcgcgtgct gccgcgtcga
     1381 tcgccttctg gacgtgcgac gggtccgcgg cgtgccagtc gccgaggacg tggcgatggt
     1441 cgtgcggcat gacggcaggc tcgacgcggc cggtccggat ggcgcacccg tcgatgacga
     1501 tgggaatctc gatc
//
LOCUS       DBNGSH010001067         4031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_169804, whole genome shotgun sequence.
ACCESSION   DBNGSH010001067 DBNGSH010000000
VERSION     DBNGSH010001067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_169804"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2840)
                     /locus_tag="AC16CY_24090"
     CDS             complement(<1..2840)
                     /locus_tag="AC16CY_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3356955.1"
                     /translation="MYSLPKNVYRERKDFSKIKTTVPIPNLIEIQRKSYERFLQMNRL
                     PAEREDAGLQSVFKSVFPISDFRENSSLEFIEYSIGNWECKCGKLAGLHHLRKPCGNC
                     GNTLVADPYGDHVVLCTRCGKENHARGDVCDICGQTVGMKLKYDVDECQERGMTYAVP
                     LKVTIRLVVWNKDPESGVKTIRDIKEQEVYFGDIPLMTDNGTFIINGTERVIVSQLHR
                     SPGAFFHSEDKTLFVAQIIPYRGSWVEFEYDQKNLLYVRIDRKRKFLATVFLRALGLR
                     GADEIIRTFHSVDRISLNQGSSLQWAVSDSLVGLRSAGDVKAGDFSIQSGKKITANAI
                     AALRKAGIETVPVADEALEGAFAAADVVDPETGEVILEANEEMTPRVISMALEKRVDK
                     IEVFFPERDEVGPIISQTLKKDPIRTHEEALIEIYRRLRPGDPPTLDSSRSLFENMFF
                     NPQKYDFSRVGRLKLNTKLGMNTPLDEKILHPQDFYEVIEYQLKLRKNPQNVDDIDHL
                     GNRRVRSVGELLENQFRIGLVRMERAIKEKMSVYQEMATAMPHDLINAKPVMAAIREF
                     FGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHPTHYGRICPIET
                     PEGPNIGLISSLSCYARINEFGFIESPYRKVKDGRVVDFVIIHNAGGHPKYKAGDVVE
                     ADEIVGADGRSKKKGVEFEPYSFYLSAWEEDQYIIAQANSPVDDNGKFTQDRVEARRA
                     GDFVLSPRADVQFIDVSPKQLVSVAASLVPFLENDDANRALMGSNMQRQAVPLLRARS
                     PYVGTGMEYITARDSGAVVVTRRTGSVDYVDSQRIVVRVEGQGEGDDVSKEMGADIYP
                     LTKFKRSNQNTCINQKPIVRVGQRVAKGQVLADGPCTELGELALGRNVLVAFMPWRGY
                     NFEDAILVSEKMVKEDYYTSIHIEEFEIEARDT"
     gene            complement(2917..3312)
                     /gene="rplL"
                     /locus_tag="AC16CY_24095"
     CDS             complement(2917..3312)
                     /gene="rplL"
                     /locus_tag="AC16CY_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR3356956.1"
                     /translation="MAELNQQQVVDYIKGISVLELSQLVKALEAELGVSAAAAVPMAI
                     PGMAAAGGGGAAAPAEEKTEFNVTLTEVGADKIKVIKVVREVTSLGLKEAKDLVESAP
                     KPIKEGVNKDESEAIKKKFEEVGAKVSIS"
     gene            complement(3329..3856)
                     /gene="rplJ"
                     /locus_tag="AC16CY_24100"
     CDS             complement(3329..3856)
                     /gene="rplJ"
                     /locus_tag="AC16CY_24100"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR3356957.1"
                     /translation="MAVTRAEKEQELQDLTAALKGADTAILVNYKGLDVPAATELRRQ
                     IRSAKGQYRVVKNTLAKRATRGTALASLEAHFEGTTAVAFTRADAVALAKALTTFMKN
                     APTLQIKAAVVGGQAVKPAAVSDLATLPGKPELYSKLLFLLQAPMQQLVTVLSATPRN
                     LVTVLNEAAKKQAQG"
     gene            complement(3890..>4031)
                     /locus_tag="AC16CY_24105"
     CDS             complement(3890..>4031)
                     /locus_tag="AC16CY_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR3356958.1"
                     /translation="HALVESVVKAKPPAAKGKYLKSVVVSSTMGPGITIDTAHIDERV
                     KH"
BASE COUNT          581 a         1352 c         1286 g          812 t
ORIGIN      
        1 gtgtcgcgcg cctcgatctc gaactcctcg atgtggatgg aggtgtagta gtcctccttc
       61 accatcttct cggagacgag gatcgcgtcc tcgaagttgt acccgcgcca cggcatgaag
      121 gcgacgagca cgttgcggcc gagggcgagc tcgcccagct ccgtgcaggg gccgtcggcc
      181 agcacctgcc ccttggccac gcgctgcccc acccgcacga tcggcttctg gttgatgcag
      241 gtgttctggt tcgagcgctt gaacttggtc agcgggtaga tgtcggcgcc catctccttg
      301 ctgacgtcgt cgccctcgcc ctgcccttcc acgcggacga cgatgcgctg gctgtccacg
      361 tagtccacgc tccccgtccg ccgcgtgacg acgacggcgc cggagtcgcg cgcggtgatg
      421 tactccatgc ccgtgcccac gtaggggctc cgggcgcgca gcagcggcac cgcctggcgc
      481 tgcatgttcg agcccatcag cgcgcggttg gcgtcgtcgt tctcgaggaa cggcacgagc
      541 gaggcggcca ccgacacgag ctgcttcggc gagacgtcga tgaactggac gtccgcgcgc
      601 ggcgacagga cgaagtcgcc ggcgcgccgc gcctcgacgc ggtcctgcgt gaacttgccg
      661 ttgtcgtcca ccggcgagtt ggcctgcgcg atgatgtact ggtcctcttc ccacgccgac
      721 aggtagaagg aatagggctc gaactcgacg cccttcttct tcgaccggcc gtcggccccc
      781 acgatctcgt ccgcctcgac gacgtcgccg gccttgtatt tcgggtggcc gccggcgttg
      841 tggatgatca cgaagtcgac gacgcggccg tccttgacct tgcggtacgg cgattcgatg
      901 aagccgaact cgttgatccg cgcgtagcac gagagcgacg agatcaggcc gatgttcggg
      961 ccttccggcg tctcaatcgg gcagatgcgg ccgtagtgcg tcgggtggac gtcgcggacc
     1021 tcgaaccccg cgcgctcgcg cgacaggccg cccggcccga gcgcggagag ccgccgcttg
     1081 tgcgtgatct cggagagcgg gttggtctgg tccatgaact gcgagagctg cgacgacccg
     1141 aagaactcgc gaatggcggc catgaccggc ttcgcgttga tcaggtcgtg cggcatggcg
     1201 gtcgccattt cctggtagac cgacatcttt tccttgatcg cgcgctccat gcggaccagg
     1261 ccgatgcgga actggttctc gagcagctcg cccaccgagc ggacgcgccg gttgcccagg
     1321 tgatcgatgt cgtccacgtt ctgcgggttc ttccgcagct tgagctggta ctcgatgacc
     1381 tcgtagaagt cctgcgggtg caggatcttt tcgtcgagcg gcgtgttcat cccgagcttg
     1441 gtgttcagct tcaggcggcc gacgcgcgag aagtcgtact tctgcgggtt gaagaacatg
     1501 ttctcgaaga gcgaccgcga gctgtcgagc gtcggtggat cgccggggcg caggcgccgg
     1561 tagatctcga tgagcgcctc ctcgtgcgtg cggatgggat ccttcttcag cgtctggctg
     1621 atgatcggcc ctacctcgtc gcgctcgggg aagaacacct cgatcttgtc cacgcgcttc
     1681 tcgagcgcca tcgagatcac gcgcggcgtc atctcttcgt tggcctcgag gatcacctcg
     1741 cccgtctccg gatcgacgac gtcggcggcc gcgaacgcgc cttcgagcgc ctcgtcggcc
     1801 acgggcacgg tctcgatgcc ggccttgcgg agcgccgcga tggcgttcgc cgtgatcttc
     1861 ttcccgctct gaatcgaaaa gtcgccggcc ttgacgtcgc cggccgagcg cagtccgacc
     1921 aggctgtccg acacggccca ctggagcgac gacccctggt tcagcgagat ccggtccacc
     1981 gagtggaagg tccggatgat ctcgtcggcg ccgcgcaggc cgagcgcccg caggaacacc
     2041 gtggccagga acttccgctt ccggtcgatg cggacgtaga ggaggttctt ctggtcgtac
     2101 tcgaactcca cccacgagcc gcggtacggg atgatctgcg cgacgaacag cgtcttgtct
     2161 tccgaatgga agaacgctcc cggcgaccgg tggagctgcg acacgatgac gcgctcggtc
     2221 ccgttgatga tgaacgtgcc gttgtcggtc atcagcggga tgtcgccgaa gtagacctcc
     2281 tgctccttga tgtcgcggat cgtcttgacg cccgactccg gatccttgtt ccacacgacc
     2341 aggcggatgg tgaccttgag gggcacggcg taggtcatgc cgcgctcctg gcactcgtcg
     2401 acgtcgtact tcagcttcat cccgaccgtc tggccgcaga tgtcgcagac gtccccgcgc
     2461 gcgtggttct ccttcccgca ccgggtgcac agcaccacgt ggtcgccgta cgggtcggcc
     2521 acgagcgtgt tgccgcagtt cccgcagggc ttgcgcaggt ggtgcagccc ggccagcttg
     2581 ccgcacttgc actcccagtt gccgatcgag tactcgatga actcgagcga cgagttctcc
     2641 cggaagtcgc tgatcggaaa gaccgacttg aacaccgact gcaggcccgc gtcctcgcgc
     2701 tcggccggca ggcggttcat ctgcaggaag cgttcgtacg atttccgctg aatctcgatc
     2761 aggttcggaa tcggaacggt ggtcttgatc ttggagaagt ccttgcgctc gcggtagacg
     2821 ttcttgggaa ggctgtacat cggttcctga tcctcgaagc ccggagaaat gggcacccgg
     2881 cctcgcggcc gggcacgtca cagctatcga tgtccgtcag gagatcgata ccttggcgcc
     2941 gacttcctcg aacttcttct tgatcgcctc ggactcgtcc ttgttgacgc cttccttgat
     3001 cggcttcggc gcgctctcga cgaggtcctt cgcttccttc aggccgaggc tggtcacttc
     3061 gcggacgacc ttgatgacct tgatcttgtc cgcgcccact tcggtcagcg tgacgttgaa
     3121 ctccgtcttc tcctcggccg gcgccgcggc tccgccgccg cccgccgccg ccatccccgg
     3181 gatggccatc gggacggccg cagccgccga cacgccgagc tccgcctcga gcgccttcac
     3241 gagctgcgac agctcgagga cgctgattcc cttgatgtag tcgactacct gctgctgatt
     3301 cagttcggcc atgtctgtct cctgctccct atccttgcgc ctgcttcttc gctgcttcgt
     3361 tcaacaccgt gaccaggttg cgcggtgtgg cggacaacac cgtcaccaac tgctgcatgg
     3421 gcgcctgcag caggaagagc agcttggaat acaactccgg cttgccggga agcgtggcca
     3481 ggtcgctgac cgccgccggc ttgaccgcct gccccccgac caccgccgcc ttgatctgta
     3541 gcgtcggcgc gttcttcatg aacgtcgtca gcgccttggc cagcgccacc gcgtcggccc
     3601 gcgtgaaggc gaccgccgtc gtcccctcga agtgcgcttc gagcgaggcg agggccgtgc
     3661 ccctggtggc ccgtttcgcg agcgtgttct tcacgactcg gtattgcccc ttggccgacc
     3721 ggatctgccg gcgcagctcc gtcgccgcgg gcacgtcgag tcccttgtag ttcaccagga
     3781 ttgccgtgtc ggcgcccttg agggcggccg tcaggtcctg cagctcctgc tctttctcag
     3841 ctcttgtgac tgccatggtt gtcttcggtc tttggtctgc ggtctacggt cagtgcttga
     3901 ctctctcgtc gatgtgcgcc gtgtcgatgg tgatccctgg ccccatcgtg gaagacacga
     3961 cgaccgactt gaggtacttg cccttggcgg ccggcggctt cgccttgacg acgctttcga
     4021 cgagcgcatg g
//
LOCUS       DBNGSH010001068         2020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate MAG05316
            SRR25158427_k127_1358379, whole genome shotgun sequence.
ACCESSION   DBNGSH010001068 DBNGSH010000000
VERSION     DBNGSH010001068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414724
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (soil metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Vicinamibacteria;
            Vicinamibacterales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,799
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1358379"
                     /isolate="MAG05316"
                     /isolation_source="soil"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="AC16CY_24110"
     CDS             complement(<1..409)
                     /locus_tag="AC16CY_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3356959.1"
                     /translation="MTVHLVNPSHLSFGVGVITPRWLFVLGAATPPAYGRPRIVDETL
                     EPLNLDTVRPGDILGIGIHTGNALRGYEIGIAARARGATVVFGGIHATLYPDEAYERG
                     GAHTVVRGDGDVVWPIVIDHAVHGTLQPRYDGGR"
     gene            1018..1227
                     /locus_tag="AC16CY_24115"
     CDS             1018..1227
                     /locus_tag="AC16CY_24115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356960.1"
                     /translation="MSIALPTVVSSPRAAVSTAVLEAASVDERWAAWQAKGAAHDRAF
                     RRKIAVAAPILIVVAAVVMYVLLGR"
     gene            1456..1653
                     /locus_tag="AC16CY_24120"
     CDS             1456..1653
                     /locus_tag="AC16CY_24120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356961.1"
                     /translation="MAVSARDLRRDLEELIAALDRRVPRVERAGEAVIARDAAALRAK
                     AVARLAELTGKAPIRAATDAR"
     gene            1670..1999
                     /locus_tag="AC16CY_24125"
     CDS             1670..1999
                     /locus_tag="AC16CY_24125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3356962.1"
                     /translation="MRTTPFPASPSDVAALTVPEFTRELDLNGSVSLDAVCGRFLEDA
                     NRAVVWFIRFRALTAWRERMDVAPWLRSTPSHAHRACELAASFDLNDAWEFDAERFRS
                     AVESVAR"
BASE COUNT          335 a          649 c          682 g          354 t
ORIGIN      
        1 cgcgcccacc gtcatatcgc ggctggagcg tgccatgcac ggcatgatcg atcacgatcg
       61 gccagaccac gtcgccatcg ccgcggacga ccgtgtgtgc gccgccacgc tcgtacgcct
      121 cgtcggggta cagcgtcgcg tgtatgcccc cgaagaccac cgtcgcccca cgtgctctcg
      181 ccgcgatgcc gatttcatag ccgcgcagcg catttccggt gtggatcccg atccccaaga
      241 tgtcgccggg gcggacggtg tcgagattga gcggctcgag cgtttcatcg acgatgcgtg
      301 gccgtccgta tgccggcggt gtggccgcgc ccagcacgaa cagccagcgc ggcgtgatca
      361 cgccaacgcc gaaggacaaa tggctggggt tcaccagatg gacggtcatc gcactccaca
      421 gcagacggag ggcaccgtcg ctttgaacac gaactgcttg aaagagactg cgctcggcat
      481 cgtcacgctc ctgcacggtc gctcagtggc tccccgatgc tcggaggctg aggcgacgtc
      541 acaatggcgt ggcaccagtc tggcgggcaa atgtagtcta gcgtacattt accggccggc
      601 ctgatcgccg ggcgtgaacg gggtgttaat cgcagctaca cggcggtcca cgtatcgacg
      661 tgcgccaggt tgataccaac ggcacagtcg gatcgggtgc agacgaaatt cgtcgagccg
      721 tgccggccgt cgtccagctt ccgctgaatt ttcgtctgcg ggtgctggca caccggacac
      781 tggtgtactg cgggccgcgc cagcgacaat ttcgagtcgg ccatgtcagt ccggatcagg
      841 agcaggggga ttgttggtga cggccttccg gcacatgacg ctgattctat ggagggaacg
      901 gggagggtgc tgttccagat tgcacagcat ggcggtggta tactggctgc tcctgctttt
      961 ctgagcccta ggccattcag gcccagccct caccaaagcc ttttgcgtga gggtcacatg
     1021 tccatcgccc tgcctactgt tgtctcctca ccgcgtgcgg ctgtgtccac ggcggtgctc
     1081 gaggcagcga gtgtcgatga acggtgggcc gcatggcagg cgaaaggcgc cgcgcacgac
     1141 cgtgccttcc gccggaagat cgcggtcgcg gcacccatac tgatcgtcgt cgccgctgtc
     1201 gttatgtacg tgctgctcgg ccgctgagcg tcatatgcgc tggcctgcga gttcacccga
     1261 cttcggccgc ggcgacgcgc aacggccgca ttcctctgca gcggccacga ccccgagtgc
     1321 gtgtccggca tgtcagtccc cttcgattac gacgacggcg cgaaacccga acgagaacac
     1381 ctattggcgg tgtggcggct gcggcgaaat ctggaacgcc tctcgacggg gggccgggcc
     1441 gagccgagcg tatcgatggc cgtgagtgcg cgcgacctca ggcgcgatct cgaggaactg
     1501 atcgcggcgc tcgaccggcg ggtgccgcga gtcgaacggg caggcgaggc tgtcattgca
     1561 cgggatgccg cggcgctgcg tgcgaaagcc gtcgcccgcc tggcggagct caccggcaag
     1621 gcccccatcc gggcggcgac tgacgcacgc tagccgggcg caggtcaaca tgcgaacaac
     1681 accgttcccg gcgagtccat ccgacgtggc ggcgctgacc gtgccggagt tcacacgcga
     1741 gctggacttg aacgggtcgg tgtcgctcga tgcggtctgc gggcggtttc tcgaagacgc
     1801 caaccgggcg gtggtctggt tcatccgatt ccgcgcgctg acggcctggc gcgaacgcat
     1861 ggacgtggcc ccgtggctgc gatcgacgcc gtcacacgcg caccgcgcct gcgagctggc
     1921 cgcgagtttt gacttgaatg acgcgtggga attcgacgcc gaacggttcc gttcggccgt
     1981 ggaatcagtc gctcgctaga cggacacgca aaggctcgag
//
